LOCUS       DATADN010000001         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_452302, whole genome shotgun sequence.
ACCESSION   DATADN010000001 DATADN010000000
VERSION     DATADN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452302"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VHH53_00005"
     CDS             <1..633
                     /locus_tag="VHH53_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288603.1"
                     /translation="DPAAADPGGRFAAACAAVPALIETRRRLDVGTVDPAALAWLDEQ
                     ARAKSQGYSLIQWVGGTPQRWLDDIAYLTGRMSTDAPLDDLQWDAEAYDAGRMRARDA
                     SCLACGLHMVTTAAVDSTGQLVAFTQIVGDSISHWYADQWDTIVAPEHRGHRLGTLIK
                     VANLEHARAQRPELRIIDTCNADTNPYMVAINEAMGFRTHRRTVEWQLDL"
     gene            802..>1750
                     /locus_tag="VHH53_00010"
     CDS             802..>1750
                     /locus_tag="VHH53_00010"
                     /inference="COORDINATES: protein motif:HMM:NF044385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2288604.1"
                     /translation="MRILFTSCPAYGHINPMLPLACEAQREGHDVAVATGAELAPEIE
                     RRGFDTWLVGPSRAETDASFRAAHPNLDVLSPEERLQVQLSGVFVDAGARRAVELVPR
                     AQEWKPDIVVHELTELAGAFAAAHTGARHVVHGLGLVPPTPLWEAFYGPGFARLCRIW
                     QVPELADGIRSATYLDICPPSLRTDGQPAWQQLQPLRPVAGDPAAGERLPAALAALPH
                     RQTIHLTLGTIFNEAAGVLETAIAGLRELSFNLVVTSGPEPPHVLIEPYIPHTLLLPR
                     CRIVVSHGGAGIMLGALAHGLPQLILPQGADQFMNASACQ"
BASE COUNT          268 a          604 c          588 g          290 t
ORIGIN      
        1 gaccccgccg ctgccgatcc tggcggacga ttcgcggcag cgtgcgcagc ggtgccggcc
       61 ctgatcgaga cgcggcggcg gctggatgtc ggcacggtag acccggctgc gctcgcctgg
      121 ctcgacgagc aggcgcgggc gaaatcgcag ggctactcgc tgatccagtg ggtgggtggc
      181 acgccgcagc ggtggctcga cgacatcgcc tacctcaccg ggcgcatgtc caccgatgcc
      241 ccgctggatg atctgcagtg ggatgcggag gcctacgacg cggggcggat gcgagcgcgg
      301 gatgccagct gcctggcctg cggcctgcac atggtcacca ccgcggcagt ggacagtacc
      361 gggcagctgg tcgctttcac gcagatcgtc ggcgactcca tatcgcactg gtacgccgat
      421 cagtgggaca ccatcgtcgc gccggagcat cgaggccacc ggctgggcac gctgatcaag
      481 gtggccaacc ttgagcacgc tcgcgcgcag cgccccgagc tgcggatcat cgacacgtgc
      541 aacgctgata ccaacccata catggttgcg atcaacgagg cgatgggttt tcggacgcac
      601 cgccggactg ttgagtggca gctcgacctg taacgccgat gatttcgata cccgcgcgag
      661 tcgatcggct ctgattgacg ccaggtgatt cgttgaattt cggtgtctcg ctgtatcaac
      721 gatggtgctg ggctggtctt ggttagcgtt cgaccgccgc ccgccgccgc acgttcgggc
      781 gttcgaggga tggagcacag catgcgaatc ctgttcacca gctgcccggc ctacggtcac
      841 atcaatccga tgctgccgct ggcttgcgaa gcccagcgcg aaggtcacga cgtggcggtc
      901 gcgaccggcg ccgaactggc gccggagatt gagcgccgtg gtttcgacac ctggctggtc
      961 ggccccagcc gcgccgagac cgacgcgtcg ttccgcgcgg cgcatccgaa cctggacgtt
     1021 ctgtcacccg aggagaggct gcaggtgcag ctgtcgggtg tgttcgtcga cgccggggcg
     1081 aggcgcgcgg tcgagctggt cccgcgggcc caagagtgga aacccgacat cgtcgtgcat
     1141 gaactcaccg agctggccgg cgctttcgcc gcggcgcaca ccggtgcccg ccacgtcgtg
     1201 cacggcctcg gactcgtgcc gccgactccc ctgtgggaag ccttctacgg gccgggcttt
     1261 gcccggttgt gccggatatg gcaggtgccc gagctggccg acggcatcag gtccgccacc
     1321 tatcttgaca tctgcccgcc gagcctgcgc accgacggcc agccggcctg gcaacagctc
     1381 cagccgctgc gcccggtagc tggcgatccc gcggcgggcg agcggttacc cgcggcgcta
     1441 gccgcgctcc cgcaccggca gaccattcac ctgacgctcg gcaccatctt caacgaggct
     1501 gccggcgtgc tggagacagc gattgccggc ctgcgcgaac tgtcattcaa cctggtcgtg
     1561 acgagcgggc cggagccgcc gcacgtgctg atcgaaccct acatccccca caccttgctg
     1621 ctcccgcgct gccgcattgt cgtctcccac ggcggtgccg gaattatgct cggcgcgctc
     1681 gcccatgggc tgccccagtt gatcctgccg caaggcgccg accagttcat gaacgcaagc
     1741 gcctgccagt
//
LOCUS       DATADN010000002         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_74, whole genome shotgun sequence.
ACCESSION   DATADN010000002 DATADN010000000
VERSION     DATADN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..932
                     /locus_tag="VHH53_00015"
     CDS             186..932
                     /locus_tag="VHH53_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX2288605.1"
                     /translation="MSLPSSRPFDHSSLVELQALLLDSGTLHDFLDRLAKLTAQALPE
                     GSSCGVTVRQNSRPVTLAASDERTGRIDQLQYDFGEGPCLETLATGTPHYIADTASEQ
                     RWPRFCPAAHEQGVRSCLGLPLSGPTGSMGGYNLYSIWPDAFAEDNREQLELFAANAA
                     GAIAVAVKLADQTQMSDDLHGALASRAVIDQATGIIMAQQRCSAAAGFGILRRASQNR
                     NVKLRDLAAEIVSNVGGAPPEPGPFQPRRS"
     gene            complement(972..1388)
                     /locus_tag="VHH53_00020"
     CDS             complement(972..1388)
                     /locus_tag="VHH53_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2288606.1"
                     /translation="MVARRCGGVGDTVTPISPGWAPEEFLSVTALSCSAQGVVVRVVG
                     EVDLATLGWLREHLHNYVSSDYRGVVLDCTEVSFLAACGTSLLVETAERARAEGMALR
                     LVTQTPLVLRALQTALADQLIPRAATVAEVVAQCAA"
BASE COUNT          315 a          576 c          535 g          305 t
ORIGIN      
        1 cctgtcggat ctagctgagg tgccgacggt cgtggtggcg gcaaccgccc ccgcgcgacg
       61 ctgaaatggt gccttcccgg aggggtagag tcagtgtgtt gccgaggttt ttcgcgcacg
      121 ccgtccggac gtggcatcta cggtagccgc tgggaacgaa gtacccacgg attgctgatt
      181 ttgccatgtc gttgccgtcg tcgcggcctt ttgatcattc gtcgctggtg gagttgcagg
      241 cgttgttgct ggacagcggc accctgcacg actttcttga ccggttggcc aagctcacgg
      301 cccaagcgtt acccgagggc agctcgtgtg gggtgaccgt gcgtcagaac tcccggccgg
      361 tcactctggc cgccagtgat gagcgaaccg gacggattga tcagctgcag tacgacttcg
      421 gtgaaggtcc gtgcctggag accctagcca ccggaacgcc ccattacatc gccgataccg
      481 ccagcgagca gcgctggcca aggttttgcc cggccgcaca cgagcagggt gtgcgctcgt
      541 gtctggggct accgctaagc ggtcctaccg ggtcgatggg tggctacaac ttgtactcaa
      601 tctggcctga cgcgttcgcc gaggacaatc gtgagcagtt agagctgttc gccgccaacg
      661 ccgccggggc gatcgccgtc gcggtgaagc tggccgatca gacccagatg tcagacgatc
      721 tgcacggggc attggcctcg cgcgcggtca ttgatcaagc caccgggatc atcatggccc
      781 agcagcgctg cagcgcggca gccggctttg gcatccttcg gcgggcctca caaaaccgta
      841 atgtcaagct ccgcgatctg gcggccgaga tcgtcagcaa cgtcggcggt gcgccacccg
      901 agccgggccc tttccaaccc cgtcgttcct gaccccagat cccgccttcc ggtctgtcgg
      961 cgacgtcggg gttacgctgc gcactgcgct accacctcag cgaccgtggc agcgcgaggt
     1021 atcaactggt ctgccaaggc ggtctgcagg gcacgcagca ccagaggagt ttgggtcacc
     1081 agccgcagcg ccatcccttc ggcgcgggcc cgctcggcgg tttcaaccag cagactggtc
     1141 ccacacgccg ccaaaaatga cacctcggtg caatccagaa ccacgccgcg gtagtcgctg
     1201 gatacgtagt tgtgcaggtg ctcccgcaac caccccaggg tggccaggtc cacctcaccg
     1261 accacccgca ccaccacccc ctgcgcagag cacgacagcg ccgtcaccga caagaattct
     1321 tccggggccc agcccggact gatcggcgtc accgtgtcac cgaccccacc gcagcgacgg
     1381 gcaaccaccg ccctcatagt tcgcgatcac gcgcactcct agcatgattt ctggaactag
     1441 gagctacgcg acccccgcgc cggggcggga caccgacacc agcgacagca gcgctcccca
     1501 tccagcatgt gctgtggaca gcggctaact gcataaccga gggcgtaaat ggtagcggct
     1561 taacagcgcc tgcggtagcg ccgatgattg accggacgtg tgagatgccc tctgaccggt
     1621 actccatacg tgaccacgac gagagatcta attagctgga gtgcttgtcg agcgaggtga
     1681 gtcagcacgg cgttgtagtc acgagcttga atctcacctc gctgcacagg c
//
LOCUS       DATADN010000003         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_301980, whole genome shotgun sequence.
ACCESSION   DATADN010000003 DATADN010000000
VERSION     DATADN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301980"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /gene="mptB"
                     /locus_tag="VHH53_00025"
     CDS             complement(<1..677)
                     /gene="mptB"
                     /locus_tag="VHH53_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEX2288607.1"
                     /translation="MVVGRVRGVGVHLGGTGSAPNALDRVQSGDPAGRTGLSQSESRQ
                     LVTVRRFGTAGALLMGLGSLGAGTAPVLGNPVVGRPLLGLLTRMPTASLAVAFTGIAM
                     VVLAWLWLGALVAPGRARLIPRAQLNRTMMMWAIPLLVAPPMFSRDVYSYLAQSAMAN
                     RGLDPYVLGPADALGVDHPLVRGIPTIWRNTAAPYGPLFLTLGRPIDSIAGDHVVLGT
                     LLHRSLAV"
     gene            769..1494
                     /locus_tag="VHH53_00030"
     CDS             769..1494
                     /locus_tag="VHH53_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX2288608.1"
                     /translation="MDVVKNAGTDRRTRESVARMLLEHGPVTAAQVADSLGLGPAAVR
                     RHLDALLADGQASYRSAPRRGPRGRGRPAKSFSLTDAGRARFEHTYDDLAVDALRFLA
                     ERDGEQAVRSFAERRVTNLVDQHRAKVAAAPDPVTRAKLLAIALSTEGYAASAEHVGA
                     GTQLCQHHCPVAHVAAEFPQLCEAETAAFADLLGVHVQRLATIARGDAVCTTHVPEAR
                     MPHGARTTVPNIPTTLPDGGSQA"
     gene            1491..>2690
                     /gene="sufB"
                     /locus_tag="VHH53_00035"
     CDS             1491..>2690
                     /gene="sufB"
                     /locus_tag="VHH53_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX2288609.1"
                     /translation="MTTAPEQDVRAQRIPTAPLTQEETLSSLGRYEYGWADSDAAGSS
                     AQRGLSEAVVRDISAKKSEPEWMLETRLKALRLFRRKPMPHWGADLSGIDFDNIKYFV
                     RSTEKQATSWEELPEDIKATYDRLGIPEAEKARLVAGVAAQYESEVVYHKIREDLEEL
                     GVQFLDTDTALREHPEIFREYFASVIPAGDNKFAALNTAVWSGGSFIYVPPGVHVDIP
                     LQAYFRINTENMGQFERTLIIVDEGAYVHYVEGCTAPIYKSDSLHSAVVEIIVKKGGR
                     CRYTTIQNWSNNVYNLVTKRATAAEGATMEWIDGNLGSKVTMKYPSVWLVGEHAKGEV
                     LSVACAGEGQHQDAGAKMVHAAPHTSSTIVSKSVARGGGRTSYRGLVQVNEGSHHSRS
                     TVKCDALL"
BASE COUNT          487 a          887 c          906 g          410 t
ORIGIN      
        1 accgcgaggc tgcggtgcag cagcgtgccg agtaccacgt ggtcgcctgc gatcgagtcg
       61 atcggccggc ccagggtcag aaacagcggg ccgtaggggg ccgccgtgtt gcgccagatc
      121 gtcgggatgc cgcggaccag ggggtggtcc acacccaatg cgtcggccgg gccgaggaca
      181 taagggtcca gtccgcggtt ggccatcgcg ctctgcgcca ggtagctgta gacgtcgcgg
      241 ctgaacatcg gtggggcgac cagcagcggg atcgcccaca tcatcatcgt gcggttcagc
      301 tgggcccggg ggatgagccg ggcacgtccg ggagcgacca atgcaccgag ccacaaccag
      361 gccagcacca ccatggcaat gccggtgaag gccaccgcca gcgaggccgt gggcatccgg
      421 gtgagaaggc ccagcagggg ccgcccgacc actgggttgc ccagcaccgg ggcggtgcca
      481 gcaccgagtg accccagtcc catgagcagc gcgccggcgg tgccgaaccg gcgcaccgtg
      541 acgagctggc ggctctcgct ctggctcaga ccggtgcgcc cggccgggtc gccggattgg
      601 acccggtcca gggcgttcgg tgcagaacca gtgcccccaa gatggacacc gacaccccgc
      661 accctcccga ccaccacgcg cgaagggtag cggtgcggcc acgcgcgccg gtgatgcaac
      721 cacaacggga cacactggcg cttggcgcac accgtggaaa tacgtaacat ggatgttgtg
      781 aaaaacgccg ggaccgatcg tcgcactcgc gagtcggtgg cacggatgtt gctggagcat
      841 ggcccagtga cagccgcgca ggttgccgac tcgctgggac tgggtcctgc tgcggtgcgc
      901 cggcacctgg acgcgctact cgctgacggc caggccagct accgctcggc accacgccga
      961 ggtccccgcg ggcgtggccg tccggccaag tcattctcgc tcaccgacgc cggccgggca
     1021 cggttcgagc acacctatga cgatctggcg gtggacgcac tgcgcttttt ggctgagcgc
     1081 gacggcgagc aggcagtccg ttcgttcgcc gaacgccgag tgacgaacct ggtcgatcag
     1141 caccgggcca aggtggctgc tgcgcccgac ccggtcaccc gggccaagct gctggcgatt
     1201 gccttgagta cagagggtta cgctgcgtca gctgagcacg tcggtgcagg tacgcagctg
     1261 tgccaacacc actgcccggt cgcgcacgtc gccgcggagt tcccgcaact gtgcgaagcc
     1321 gagacggcgg cctttgctga cctgctcggg gtccatgtgc agcggctggc caccatcgcg
     1381 cgcggcgacg cggtatgcac gacgcacgtt cccgaggccc ggatgccgca cggtgcccgc
     1441 acgactgttc ccaacatccc caccacgctc ccggatggag gctctcaagc atgacaactg
     1501 ctcctgagca ggatgttcga gcccagagaa tcccgaccgc accgctcacc caggaggaaa
     1561 cgctctccag tctgggccgc tacgagtacg gctgggccga ctctgatgcc gccggctcca
     1621 gcgcgcagcg tggcctgtcc gaagctgtgg tccgggacat ctcggccaag aagagtgagc
     1681 ctgagtggat gctggagacc cggctcaagg cgttgcgcct gttccggcgc aagccgatgc
     1741 cgcactgggg cgctgaccta tccgggatcg acttcgacaa catcaagtac ttcgtccggt
     1801 cgaccgagaa gcaggccacg tcctgggaag aactgcccga ggacatcaag gccacttacg
     1861 accggttggg cattcccgag gccgaaaagg cgcggttggt ggctggtgtg gccgcgcagt
     1921 acgagtccga ggtcgtctac cacaagatcc gcgaggacct tgaggagctg ggcgttcagt
     1981 tcctggacac cgacaccgca ttgcgcgagc acccagagat attccgggag tacttcgcca
     2041 gtgtgatccc ggccggtgac aacaagttcg ccgcgttgaa cactgcggtg tggtccggtg
     2101 ggtcattcat ctatgtgccg cctggcgtgc acgtcgacat cccgctgcag gcctacttcc
     2161 ggatcaacac tgagaacatg ggccagttcg agcggacact gatcatcgtc gacgagggcg
     2221 cttacgtgca ctacgtcgaa ggctgtacag cgccgatcta caagtccgac tcgttgcact
     2281 ccgcggtcgt cgagatcatc gtcaagaagg gtggccgctg ccgctacacc acgatccaga
     2341 actggtcgaa caacgtctac aacctggtca ccaagcgggc gaccgccgct gaaggcgcga
     2401 ccatggagtg gattgacggc aacctgggct ccaaggtcac catgaaatac ccgtcggtat
     2461 ggttggtcgg tgagcacgcc aagggtgagg tgctgtcagt agcctgcgct ggcgagggcc
     2521 agcaccagga cgccggggcg aagatggtgc acgccgcacc gcatacctcg agcacgatcg
     2581 tgtccaagtc ggtggcccgc ggtggtggcc gcacctccta ccgcgggctg gtgcaggtca
     2641 acgagggcag tcaccactct cgctccacgg tgaagtgcga cgccctgctg
//
LOCUS       DATADN010000004         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_603631, whole genome shotgun sequence.
ACCESSION   DATADN010000004 DATADN010000000
VERSION     DATADN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603631"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VHH53_00040"
     CDS             <1..271
                     /locus_tag="VHH53_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288610.1"
                     /translation="LPERADLHAQLSATIAAHQISDGTWSQADLFHTLEALLALGTPQ
                     ARASVRRAVPTLLARQRSDGSFGSTAQQERAWIALRALIWADQNG"
     gene            293..1366
                     /gene="selD"
                     /locus_tag="VHH53_00045"
     CDS             293..1366
                     /gene="selD"
                     /locus_tag="VHH53_00045"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:359..361,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX2288611.1"
                     /translation="MADAPIDQKPRIRLTSLSHGAGUACKLGSADLAQVLRLLPSIGD
                     PRVLVDATTKDDAAVFLLAPDRALVATVDFFTPIVDDPAAWGAIAAANALSDIYAMGA
                     SPLFGLNLVGWPRDRVPFEMLGEVLRGAAEVAGRARCLVLGGHTIDAVEPTFGMAVIG
                     EVHPDRMLTNAGACTGDALVLTKPLGTGILATALKRDALIETGMAEAVRSMTTLNDGA
                     ARAALKVGVSAATDVTGFGLIGHLTGVLDASRVAAELAFDAIPLLPHARNLAARGVIP
                     GGTQRNLEAADRVEWAADLSTAERHLCVDAQTSGGLLLAVPAEHEAALLLALREENTP
                     AAAVIGRLVSGSPGTIRVSRKLG"
BASE COUNT          269 a          511 c          529 g          265 t
ORIGIN      
        1 gctgcctgag cgcgccgacc ttcacgctca gctctccgcc acgattgccg cccatcagat
       61 cagtgacggc acctggtctc aggcggatct atttcacacc ctggaggcac tgctggcact
      121 gggaactccc caggcgcggg catcggtccg ccgtgcggtc cctactctgc tggcacgcca
      181 gcgttccgac ggcagcttcg gatccactgc tcagcaggaa cgggcgtgga ttgccctgcg
      241 cgcgttgatc tgggccgatc agaacggcta gactgcgcag ggaggaggct gtatggcaga
      301 cgcaccaatc gatcagaagc cgcgaatccg tttgacgtcg ctctcgcacg gcgccggctg
      361 agcctgcaag ctcggctcag ccgatctggc gcaggtcctg cgcctcctgc cgagtattgg
      421 tgacccccgg gtcctggttg acgcaaccac caaggatgac gccgcggtat tcctgctggc
      481 gcccgaccgt gcgctggtgg cgacggtgga cttctttacc ccgatcgttg acgaccccgc
      541 agcctggggt gccatagccg cggcaaatgc gctgagtgac atctacgcga tgggcgccag
      601 cccgcttttc ggtctgaacc tggtgggatg gccccgggac cgggtgccgt tcgaaatgct
      661 gggagaggtg ctgcgtggtg ccgcggaagt tgcgggacgg gcccgctgcc tggtcctggg
      721 aggccacacc attgacgcag tcgagcccac gttcggcatg gcggtgatag gcgaagtaca
      781 ccccgacagg atgctcacca acgcaggcgc ctgcaccggt gatgctctgg tactcaccaa
      841 gccgctgggt accggcattc tggccaccgc gctcaagcgg gatgccctga tcgagaccgg
      901 catggcggag gcggtgagat cgatgacgac gctcaatgac ggcgccgccc gtgcggctct
      961 gaaggtgggc gtgagcgcag ccacggacgt caccggcttc gggctcatcg gccatttgac
     1021 aggggtgctc gacgccagca gggtcgccgc cgagctggca ttcgatgcca tccctctgct
     1081 cccgcacgcc agaaacctgg ctgctcgcgg cgtcattccc gggggcaccc agcgcaacct
     1141 cgaggcagcc gatcgggttg agtgggccgc ggatctcagc acggcggagc gccatctctg
     1201 cgtagatgcc cagacctccg gcggactgct gctggctgtg cctgccgaac acgaagccgc
     1261 actcctgctg gccttgcgcg aggaaaatac tccggccgct gcggtgattg gccgcctcgt
     1321 aagcggatcc cccggcacga tccgggtaag tcgaaaactc ggctgaccgg ctatcgttcc
     1381 gactccaggg gccgtatggc gaaatcgagt tataagtgga agtgcggaga ggaaaatgac
     1441 gcaattgcgg tggaccagcg aacgcatgga tcaactggag cgggccgccc gggatgggct
     1501 ccgggtagcc ctcgctcgca gaggcaccga atacgtcgtc gtcgcgcagc gggtgacgtc
     1561 agtagaccag cacg
//
LOCUS       DATADN010000005         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_452874, whole genome shotgun sequence.
ACCESSION   DATADN010000005 DATADN010000000
VERSION     DATADN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452874"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..1095
                     /locus_tag="VHH53_00050"
     CDS             349..1095
                     /locus_tag="VHH53_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX2288612.1"
                     /translation="MSLPSSRPFDPSWLVELQALLLETNTLYDFLDRLAQLTAQTLPE
                     GSSCGVTVCQDSRVVTLAASDEQARRIDQLQYDLDEGPCLDTVATGKPHYIADTLSEA
                     RWPSFCPAVHEQGVRSCVGLPLSGPSGLMGGYNFYSTGPDAFAQDTRRQLDMFAANAA
                     GAIAVAVKLADQTQMSDDLHGALASRAVIDQATGIIMAQQRCSAAAAFDVLRRASQNR
                     NIKLRELAAELVRNVGGKPPEPGPFQPRRS"
     gene            1228..2181
                     /locus_tag="VHH53_00055"
     CDS             1228..2181
                     /locus_tag="VHH53_00055"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX2288613.1"
                     /translation="MLMLVDREEISRGLVEGLQYKEIACRIRRDPSVISREVARHGGR
                     SDYRAACVARERPKRLAVERSLRLRALVCRQLRGGWSPASIAGRLSTDYPDDQACRVS
                     HEAIYQWVYAQPVSTLARELISLRTGRKARSGGRRPPAAPRIREPRYIDERPAEATDR
                     ALPGHWEGDLVIGKDGRSAVATLVERTSRFLLLVPLTGRDALTVGDAVIAATGGLPAA
                     IRRSLTWDCGAEMARHATITATGLPVFFAHPHSPWERGSNENLNRIVREFFPKGVTIT
                     SDPHYLAMVASEINDRPRKIHNWKKPSEQFTELVKAHASTG"
BASE COUNT          364 a          695 c          773 g          424 t
ORIGIN      
        1 gtggcgagtc ggtctcggtg ctctgcacgc tcccggccaa ggtccaacca ggacggggac
       61 cacggcatca gccgaggaac tccttccagg gcgagatcag caaggacacc gctggcgtgg
      121 cgtgggccaa gctcggcggc cgcccgcgat ccggtacgac actgtgtcgg gtgtagctgg
      181 ggtgctgacg gtcgtggtgg cggcaacctc cagcgcgacg ctggaatggt gcgccgcgcc
      241 gggaggggat agcttggtcg agttgccggg ggttttcgcg cacgtcgttc ggacgtggca
      301 tctgcggtag ccgttgggta cgagataccc cggattgctg gttggtctat gtcgttgccg
      361 tcgtcgcggc cttttgatcc ttcgtggttg gtggagttgc aggcgttgct gctggaaacc
      421 aacacgctgt acgactttct tgatcggttg gcccagctga cggcgcaaac gttgcctgag
      481 ggcagctcgt gtggtgtcac cgtgtgtcag gactctcggg tggtcactct ggccgccagt
      541 gatgagcagg ccaggcggat tgatcagctg cagtacgacc tcgatgaagg tccatgcctg
      601 gacactgtgg ctactggaaa gccccattac atcgccgata ccctcagcga ggcgcgctgg
      661 ccgagtttct gcccggccgt acacgagcag ggtgtgcgct cgtgtgtggg gctgccgcta
      721 agcggtccta gcgggctgat gggtggctac aacttctact cgacggggcc tgacgcgttt
      781 gcccaggaca ctcgcaggca actcgacatg ttcgccgcga acgccgctgg ggcgatcgcc
      841 gtcgcggtga agctggccga tcagacgcag atgtcagacg atctgcatgg ggcgttggcc
      901 tcgcgtgcgg tcattgatca agccacgggg atcatcatgg cccagcagcg ctgcagcgcc
      961 gcggccgcct ttgacgtcct tcggcgggcc tcacaaaacc gcaatatcaa gctccgcgag
     1021 ctggcggctg agctcgtcag aaacgtcggc ggtaagccac cagagccggg ccctttccaa
     1081 ccccgccgct cctgacctca gatcagtcgg agcctcagga ggccgtgatg acagagtcga
     1141 tcaagctcgg cattcctgcc tgggctcagc cagtcgtagc ttttgatctt ggaagttcgg
     1201 tgggacagga ttcccgccgt gcgagagatg ttgatgttag tggatcggga ggagatctcc
     1261 cgtggtctgg tcgagggctt gcagtacaag gagatcgcgt gtcggatccg gcgtgatccg
     1321 tcggtgattt ctcgggaggt cgcgcgtcac ggcggccgca gcgactaccg ggcggcgtgt
     1381 gtggctcggg agcgtccgaa gcggttggcg gtcgagcgct cgctgcggct gcgggcgctg
     1441 gtgtgtcggc agctgcgtgg tggctggtcg ccagcgtcga tcgcgggtcg gttgtcaacc
     1501 gattaccccg acgaccaggc ttgccgggtg tctcacgaag cgatctacca gtgggtctac
     1561 gcccaaccgg tctccaccct ggccagggaa ctgatcagtc tgcggaccgg gcgcaaggcc
     1621 cggtcgggtg gtcgtcgccc gccggctgcc ccgcggattc gcgagccccg ctacatcgac
     1681 gaacggcccg ccgaggcgac cgaccgggca cttcctgggc actgggaggg agacctcgtc
     1741 atcggcaagg acggccgctc cgcggtggcg acgctggtgg agcggacctc acggtttctg
     1801 ctcctggtgc cgttgaccgg ccgggacgcg ttgaccgtcg gtgacgccgt catcgctgcc
     1861 accggtggac tgcccgcggc gatccgccgg tcgctgacct gggactgcgg cgctgagatg
     1921 gcacgccacg ccactatcac cgcgaccggg ctgccggtgt tcttcgccca cccgcactca
     1981 ccgtgggaac gcggcagcaa cgaaaacctc aaccggatcg tccgcgaatt cttccctaaa
     2041 ggcgtgacga tcacctctga tccccattat cttgccatgg tagcctccga aatcaacgac
     2101 cgaccccgta agatccacaa ttggaagaaa ccctccgagc aattcactga acttgtcaag
     2161 gcgcatgctt ccactggctg aatctgcccc cacgtgatgc gcgcgatgcg ggggcagctg
     2221 agtagccacg tgaccgtgaa gcgttgggtg cagcgg
//
LOCUS       DATADN010000006         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_770, whole genome shotgun sequence.
ACCESSION   DATADN010000006 DATADN010000000
VERSION     DATADN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..200
                     /locus_tag="VHH53_00060"
                     /pseudo
     CDS             1..200
                     /locus_tag="VHH53_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(189..1850)
                     /locus_tag="VHH53_00065"
                     /pseudo
     CDS             complement(189..1850)
                     /locus_tag="VHH53_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
BASE COUNT          283 a          645 c          652 g          270 t
ORIGIN      
        1 gagcggccga cgtcgtgctg ccaatcgcgc cagcggtcga gaaggccggc agctacctcg
       61 actgggaggg ccgccgccgc gagttcacca ccacgatcaa tacctcgggg attggtgcgg
      121 cgctgccgga ctgccgcgtg ctggacagcc tcgccgtcga gatggacgtc gatctgttca
      181 cccagacccc ggcggcggcc ctcccagtcg aggtagctgc cggccttctc gaccgctggc
      241 gcgattggca gcacgacgtc ggccgctcgg gtcacggccg agggccgcat ctccaggctc
      301 accacgaatg tggcccggtt cagcgcctgc tcggccagcc gggggtccgg gagatcggcc
      361 gggtctacgc cgccgacgac cagcccgtcc agctgcccgg ccgctgcggc ggcgaggatg
      421 ccggcagtgt cccggccagg ctcgctggac agcgtcacgc cccaggcgcg ctcgatctcg
      481 gcacgggcct gcgggtcggt gaccggccgg ccgccgggca gcaggtttgg cgccgcaccg
      541 gcctccaggg cacctcgctc gccggctcgt cgcgggatcc aggcgatccg ggcaccggta
      601 cccgcggcca atcgggccac tgccgagtac agcccgggta cctcagcggc gcgctcgccg
      661 accagcagca ccgcccccgg ctcgcgcaac gcgtccagtg ccggaccctc cagcgcgtcc
      721 agggcgtgcg gctcaccgcc gggtggcacc cgcagcaacg tgccgcgcgt tttacgcacc
      781 gcgggcgtgg tgaacggccc gaggtgaaac accggcatgg cacggtcccg ggcggcgcga
      841 cgcagccgca ggaagacgat tggcgactcc tcctcgggct cgaaggccac gcagagcgcc
      901 gcaggggcac cactgaggtc gtcgtaggtg accccaccgg tgtgcggggt gaccccgacc
      961 acgtgtgcgg cgaaaaagtc tagctcctcg gccgagtgtg gtcgggcgcg gaagtcgatg
     1021 ttgtgggtgc ccagcgcgac cctagcgaac ttcgcatagg cgaaggcgtc ttccatggtg
     1081 agccggccgc cggtgagcac cccggtgccg cggtcccgga ctgcaagcag tccctcggcg
     1141 gccgcctgca gcgcctctgt ccacgacgat ggctgcaggg caccgtagga gtcccgcacc
     1201 agcggtcgag tgatccgatc ctcggctctg gcgtaggtga aggcgaaccg gcccttgtcg
     1261 cagttccact cctcgttgac ctgcgggtca taaccggcga gccggcgctg cacgatgccc
     1321 cggcgccagt cggtgcgctg cgcgcagccg ctcgcgcagt gttcgcagac cccgggggtg
     1381 gagacaaggt cgaagggtcg ggagcggaac cggtaggcgg cgctggtgag tgcaccgacc
     1441 gggcaaattt ggatggtgtt gccggagaag taggactgga aaggcttctg ggctgccacc
     1501 ccgacttgct gggaggcacc gcgctcgagc atctcgataa acggatcccc ggcgatctgc
     1561 tcagagaaac gggtgcagcg cgcgcacagc acgcagcgct cccgatccag cagcacctgg
     1621 ctggagatcg caatcggttt ggcaaaggtt cgtttggtgt cgatgaaccg ggagtctgcc
     1681 cggccattgg acatcgcctg gttctgcagc gggcactcgc cgcccttgtc acagaccggg
     1741 cagtccagcg ggtgattaat cagcagcagc tccatcacac cctgctgagc cttgtccgcc
     1801 accgccgacg tgtgctgggt cttgatgacc atgccatcgg ccacggtgag
//
LOCUS       DATADN010000007         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_151553, whole genome shotgun sequence.
ACCESSION   DATADN010000007 DATADN010000000
VERSION     DATADN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151553"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VHH53_00070"
     CDS             complement(<1..773)
                     /locus_tag="VHH53_00070"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEX2288614.1"
                     /translation="MNAEKGVPQEILIQTLEQAIESAYRRNADAPENVVVHVDPESGQ
                     ISVFREYEVVAGEAEIEDPELQMVAAEAQKIDPSTGVGGKVRVPENVTQAQLGRIAAQ
                     TAGQVYRQRLREAERDVVYSQYASREGEIMTGVVHRTTDQAVVLDMGKGVEAILAVTE
                     QLPGEHYRIGQHLKVYVLEVRRTARGPVLVVSRTHRGFLRRLMEMEIPEIYNGTVVIR
                     GIAREAGSRSKVAVESRQPGVDAKGAAVGQRGARIQAIVA"
     gene            complement(1133..1513)
                     /locus_tag="VHH53_00075"
     CDS             complement(1133..1513)
                     /locus_tag="VHH53_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288615.1"
                     /translation="MLRASRLLLALATAGLILAASALPVTARDACPNGGFQSAQLLNA
                     DGSLNMAVVDDYPSILRAFADQIYSPDVFAADVAALDKNGNGVLCIKDIWEHNGGHGG
                     PPEPDQAQGLGGFFYFVHGIDDKL"
     gene            complement(1700..>1920)
                     /locus_tag="VHH53_00080"
     CDS             complement(1700..>1920)
                     /locus_tag="VHH53_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2288616.1"
                     /translation="FVGLLSVNLAVLNVLPFPPLDGGRIAVVLIEALRRRRLPAEREA
                     LIYLTGFMVLIALVILISIQDVQRLIQG"
BASE COUNT          306 a          656 c          626 g          332 t
ORIGIN      
        1 gccacgatgg cctggatccg cgcgccacgc tgccccaccg ccgcgccctt ggcgtctacc
       61 cccggctggc ggctttcgac ggccaccttg gagcggctgc ccgcctcgcg ggcgatgccg
      121 cggatgacca ccgtgccgtt gtagatctcg gggatctcca tctccatcag gcgtcgcagg
      181 aagccgcgat gcgtgcggct cacgaccagg accgggccgc gtgccgtgcg gcgcacctcg
      241 aggacgtaca ccttcaggtg ctgcccgatg cggtaatgct ccccgggcag ctgctcggtg
      301 acggccagga tggcctcaac gcccttgccc atgtcgagga caacggcctg atcggtggtt
      361 cggtgcacca cgccggtcat gatctcgccc tctcgcgagg cgtactggct gtacaccacg
      421 tcgcgctcgg cctcgcggag gcgctgccgg tagacctggc cggcggtctg cgccgcgatc
      481 cggcccagct gggcctgggt cacgttctcc ggcacccgca ccttgccgcc cacgccggtc
      541 gaggggtcga tcttctgggc ctcggcagca accatctgga gctccggatc ctcgatctcg
      601 gcctcccctg ccacgacctc gtactcgcga aagacgctga tttgcccgct ctcggggtcc
      661 acgtgcacga ccacgttctc gggcgcatcg gcgttgcgac ggtaggcgga ctcgatggcc
      721 tgttccaggg tctggatcag gatctcctgc ggcacgccct tctcggcgtt caactggagc
      781 agagctccga tgaagtctcg attcacgtcc gcacctcgcg cccatccggg cagaaacaaa
      841 aacgtgggat ggcgaaccga cccacgtttc cctcagtcag tatcgattcg gctcgctctc
      901 tttcggtcgg cgaaaccgtc ccgaagtata tcagcgacca cccctaggcg ccaaacggca
      961 cccggcgaag cactggcttc atcggtcctc cgagtgagca gcgcggggat cacctgatcg
     1021 accgttcgca ctccctgcga ccgggcgtcg tgcggtgtcc ccgccctgcg cgatccccga
     1081 gcgccatcaa cggcggttca agatcggatg ggggcggcat ctgggtgccg acctagagct
     1141 tgtcgtcaat cccgtggacg aagtagaaga acccgcccag cccctgcgct tggtcaggct
     1201 ccggcggccc cccatgtccg ccattgtgct cccagatgtc cttgatgcac agaacgccat
     1261 tgccgttctt atccagcgcc gccacatcgg ccgcgaacac atccggcgaa tagatctggt
     1321 ccgcgaatgc gcggaggatg ctgggatagt cgtccaccac cgccatgttc aggctgccgt
     1381 ccgcattcag cagctgtgcg gattgaaagc cgccgttcgg gcacgcgtcg cgggccgtga
     1441 cgggcagcgc actcgccgcg aggatgagac cagctgtggc aagagcgagc aagagacgtg
     1501 atgcgcggag catggcttgg cactccaaaa cccgtggcgg gtcggtttcg ccagtagctc
     1561 agtgcgtccc ccggcggcca tcggtatggg cccgcacgtc gtcgcttccc gcttgtgcgg
     1621 ttcgttgtac tcgagtgcgg ttcagttggc tagccccgga gcggccgggt ggtgcgggtt
     1681 ggcaacggtc gaaggtcgtt cagccctgga tgaggcgctg gacgtcctgg atggagatga
     1741 ggatgaccag ggcgatgagg accatgaagc cggtgaggta gatgagcgct tcccgctcgg
     1801 cgggtaggcg gcgccggcgc aaggcctcga tcagcaccac cgcgatgcgc ccgccgtcga
     1861 gtggcgggaa aggcagcacg ttaagcaccg ccaggttgac cgacagcagt cccacgaaga
//
LOCUS       DATADN010000008         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1056502, whole genome shotgun sequence.
ACCESSION   DATADN010000008 DATADN010000000
VERSION     DATADN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056502"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VHH53_00085"
     CDS             <1..1211
                     /locus_tag="VHH53_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HEX2288617.1"
                     /translation="GAVPGGDDGTAASEYAGFETVGIPDRPPQTEREAEMYSLEEGEM
                     LLNMGPQHPSTHGVMRLVVKIRGEVVTDLDPVLGYLHRGIEKLCENATYTTVLTYVDP
                     MDYLSAMHNEHAPAIAFEKLYGIKAPPRAEYIRALVVELNRVFSHGLMMGFLALDMGG
                     LTPILYAFINRDEVVDLLSAISGQRMLFNFFRVGGVNWDVNDEFINRLSKWRVNVGKS
                     VGDNEALITQNEIFTQRTKGMGVLTGQEAISLGVSGPNLRASGVPFDVRKAHPYSIYP
                     ELEFDVITQSSGDSYARYLQRVAEIKQSLHLIDQIVDRMPHGPVQAQVPGIIRPKPGR
                     AFAAIESPRGFYSVYAISDGGPTPYRFRMRDPSFITLQAMPKLMPGHLIADTMAIMAS
                     LDPVMGGVDK"
     gene            1224..>1508
                     /locus_tag="VHH53_00090"
     CDS             1224..>1508
                     /locus_tag="VHH53_00090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX2288618.1"
                     /translation="MDVGELLLGIGRFVLATTLLLLLTVPTAFIVIQMELKFIAQMQL
                     RLGPNRVGPWGMFQSTIHGFKVLAKEDTTPDQADRPTFTMAPWMVFMAAAM"
BASE COUNT          272 a          508 c          485 g          243 t
ORIGIN      
        1 tcggcgcggt gcccggtggc gacgacggca cagccgccag cgagtacgcc ggcttcgaaa
       61 cggtcggcat cccggaccgg ccgccgcaga ccgagcgcga ggcggagatg tactcgctcg
      121 aagagggcga gatgctcctc aacatggggc cgcagcaccc gtcaacccac ggagtgatgc
      181 gcctggtggt caagatccgc ggcgaggtgg tcaccgacct tgacccggtg ctgggctacc
      241 tgcaccgcgg catcgagaag ctgtgcgaga acgccaccta caccacggtc ctgacctacg
      301 tcgaccccat ggactaccta tcggcaatgc acaacgagca cgcgccggcg atcgccttcg
      361 agaagctgta cggcatcaag gccccgcccc gggcggagta catccgggcg ctggtggtcg
      421 agctgaaccg ggtgttcagc cacgggctga tgatgggctt cctggcgctg gacatgggcg
      481 gcctgacccc catcctgtac gccttcatca accgtgacga ggtggtcgac ctgctgtcgg
      541 ccatctccgg ccagcgcatg ctgttcaact tcttccgagt cggcggcgtg aactgggacg
      601 tgaatgacga gttcatcaac cgcctgtcca agtggcgggt caacgtgggc aagagcgtcg
      661 gcgacaacga ggcgctgatc acccagaacg agatcttcac ccagcgcacc aagggcatgg
      721 gcgtcctaac cggccaggag gccatctcgt tgggcgtatc gggcccgaac ctgcgcgcat
      781 cgggcgtgcc cttcgacgta cgcaaggcgc acccctactc tatctacccc gagctcgagt
      841 tcgacgtcat cacccaatcg agcggtgaca gctatgcgcg ctacctgcag cgagtggccg
      901 agatcaagca gagcctgcac ctgatcgacc agatcgtgga caggatgccg catgggccgg
      961 tgcaggccca ggtgcccggc atcattcggc cgaagccggg gcgtgccttc gctgccatcg
     1021 agtcgccacg cggcttctac tcggtgtacg ccatcagcga cggcggaccg acgccctacc
     1081 gattccgaat gcgcgatccg tcgttcatca ccctgcaggc gatgcccaag ctgatgccag
     1141 gccacctcat cgccgacacc atggccatca tggccagcct ggacccggtg atgggcggcg
     1201 tggacaagta gcgggccgag cagatggatg ttggcgagct gctgctgggc atcggccgct
     1261 tcgtgctggc caccacgctg ctgctcctgc tgaccgtgcc gacggccttc atcgtcatcc
     1321 agatggagct gaagttcatc gcccagatgc agctccggct cgggccgaac cgcgtgggtc
     1381 cctggggcat gttccagagc acgatccacg ggttcaaggt gctggccaag gaggacacca
     1441 cgccggacca ggcggatcgg cccaccttca ccatggcccc ctggatggtc ttcatggcgg
     1501 cggccatg
//
LOCUS       DATADN010000009         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_604421, whole genome shotgun sequence.
ACCESSION   DATADN010000009 DATADN010000000
VERSION     DATADN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604421"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..1973
                     /locus_tag="VHH53_00095"
     CDS             495..1973
                     /locus_tag="VHH53_00095"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX2288619.1"
                     /translation="MAIFEYGVASGDPLTDRVVIWTRVESDQPSAEVTWVVALDQEMI
                     NVVATGTAMAQPQHDHTVHVDVAGLEQDTYYWYKFRAMGEDSPIGRTRTLPADPSSVR
                     FAYTSCAKYNAGFFNVYARIADRDDISFLLHLGDYIYETSETPPASQTPSRNIGRPFD
                     PPHECLTLADYRVRYRQYRRDPDLQALHLRHPIIQTTDDHEVADGAWRDGAQEHKAEY
                     GPWAQRKLEAFQARYEWLPIRNPDPDDPLRVWRSIPLGSLADIILIETRLHRDEPVAG
                     DAMKDPSRSVLGPDQRAWLWETIQSSTARWRVLGNPSVMAQTWLPDLPEDIKMPLEKV
                     KLIDRGVDGPDYDQWDGYPAERKQLLDLIESRGRGTSIVLSADVHVGLAIELNQDAYE
                     RDEPVCVELVSPSITSQNLDDKMKWGYRVESLPLEARILESLPHWRWVDLDSHGYVVV
                     DLDADRVHAEFWGVPTVLKRVNGETLLASFETRHGDPHLTKL"
     gene            2273..>2554
                     /locus_tag="VHH53_00100"
     CDS             2273..>2554
                     /locus_tag="VHH53_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288620.1"
                     /translation="MSPYGRPVARGRCLATTLCYPPESLAAYKHALRLMDEHATDTPE
                     LRALALAGLAWVETASGDVDRAQRLIEQVQALPEVADDAVLNAELRIARA"
BASE COUNT          443 a          855 c          834 g          422 t
ORIGIN      
        1 ttcggatggt cgctgacctg ccctaatagc tccgcaccgc agggggcttt taattccctt
       61 gctccgtagt ggcgactgca cgggacatct tggctgctcc actgtctacc tgctggttgt
      121 ccatgtcgga ggcagcgttg ccggccgcag tgagccaacg ccgtgctgaa gtcgggctcc
      181 tttgttacgc gaaggcacga gactggcagc tgctcagcgg gggagtggcg cgtgaccaca
      241 ggatccgggc tcgttgatca aatctgagtc acgccgctca cggtgcgctg cctgccctga
      301 gccccaacca gataggccag ctgaccgccg acctcgtcta cgggaacctg accaggagcc
      361 cggtcagtgc tcacctcgac ggggtgtggg ctgcgctcga agcttcgacg tcggtgcgag
      421 cctttcggag tcgggtagct tcggtcaagt taatgtcttg ctcgtgctgc gggagcagac
      481 gaggaaaggg cgggatggct atcttcgagt atggggtcgc gtcgggcgac ccattgaccg
      541 accgcgtcgt catctggacg cgggtcgaga gcgaccagcc cagcgctgag gtgacctggg
      601 tcgtggcgct tgaccaggag atgatcaacg tcgtcgcaac cggcaccgcg atggcgcagc
      661 cgcagcacga tcacaccgtg cacgtcgacg tggcgggcct ggagcaggac acctattact
      721 ggtacaagtt ccgggccatg ggtgaggaca gccccatcgg gcgcacccgt accctgcccg
      781 ccgatccgtc gagcgtccgc ttcgcctaca catcctgcgc caagtacaac gccggcttct
      841 tcaacgtgta cgcgcgcatc gcggaccgcg acgacatctc attcctgctc cacctcggcg
      901 actacatcta tgagacgtcg gagacgccgc cagcgagcca aacaccgagc cgcaacatcg
      961 gtcgcccgtt cgaccccccg cacgagtgcc tcaccctcgc cgactaccgg gtgcgctacc
     1021 gccagtaccg cagagatcct gacctccagg cattgcacct gcgccacccg atcatccaga
     1081 ccaccgatga tcacgaggtg gccgacggcg cgtggcgcga cggcgcccag gagcacaagg
     1141 ccgagtacgg accgtgggca caacgcaagc tcgaggcatt ccaggcccgc tacgagtggc
     1201 tgcccattcg taatcccgac cccgacgacc cgttgcgggt gtggcggtcg atccccctcg
     1261 gctcgctcgc cgacatcatc ctcatcgaga cgcggttgca tcgcgatgag cccgtggcag
     1321 gcgacgcgat gaaggacccg tcgcgctcgg tcctaggccc cgaccagagg gcctggctgt
     1381 gggagacgat tcagtcgtcc acggcgcgct ggcgcgtgct cggtaaccct tcagtgatgg
     1441 cccagacgtg gttgcccgac ctgcccgagg acatcaaaat gccgctagag aaggtcaagc
     1501 tgatcgaccg cggcgtggac ggcccggact acgaccagtg ggacggttac ccagccgagc
     1561 gcaagcagct gctcgacctg atcgagtcgc gggggcgggg cacgtcgatc gtgctgtcgg
     1621 ccgacgtgca cgtcggcctg gccatcgagc tcaaccagga cgcctacgag cgcgacgagc
     1681 cggtgtgcgt cgagttggtg agcccgtcga tcacctcgca gaacctcgat gacaagatga
     1741 agtggggcta ccgcgtcgag tcgctcccgc ttgaggccag gatccttgag tcgctccccc
     1801 attggcggtg ggtcgacctc gacagccacg gctacgtggt cgtcgatctg gacgccgatc
     1861 gcgtccacgc cgaattctgg ggcgtgccaa cggtgctcaa gcgggtgaac ggtgaaactc
     1921 tcctggcttc cttcgagacc cgccatggcg acccgcacct caccaagctc tgacatggaa
     1981 cgcaggccag tggggccgag gttgtcccgg gactcgtgga agttgaggcg gcgcccccac
     2041 ttgactcctg ccgtgcggta ggtactcggg tgaaccgcca agaacaccac gaggaagggg
     2101 accgccgtgc gcacccccgc cgctggccag ccgggttctc ggcgcgaacg cgccgggtag
     2161 ccggacgaca cgaactttgt ccggcccacc agcccaaatg gtccgattgc ggcagaatgc
     2221 tgtcgctgcc gtttgttgtc agcggtcggt gcatagaggg atagggaggc gcgtgtcccc
     2281 ctacggcagg ccggtggccc gcggccgctg cctggccacc acactctgct acccaccgga
     2341 gtcgctggcc gcctacaagc acgccctgag attgatggac gagcacgcca ccgacacgcc
     2401 ggagttgcga gcactcgctc ttgcgggtct tgcctgggtg gagaccgcaa gcggcgacgt
     2461 ggaccgcgcg cagcgcctga tcgaacaggt gcaggctctg ccggaggtcg ctgatgacgc
     2521 cgtgttgaac gccgagctgc ggatcgcccg tgcc
//
LOCUS       DATADN010000010         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1207516, whole genome shotgun sequence.
ACCESSION   DATADN010000010 DATADN010000000
VERSION     DATADN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207516"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..920
                     /locus_tag="VHH53_00105"
     CDS             516..920
                     /locus_tag="VHH53_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX2288621.1"
                     /translation="MSGRIRVVVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLHQTP
                     EQIVETALQEDADAVGLSVLSGAHMTLFARVIELLQSKGAADVLVFGGGIIPDEDIPK
                     LTELGVAKVFTPGATTHEIINWVRAALATPVE"
     gene            1015..>1584
                     /locus_tag="VHH53_00110"
     CDS             1015..>1584
                     /locus_tag="VHH53_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX2288622.1"
                     /translation="MDLYEYQAKDLFQAHGVPVLPGRVATTSAQARACAEELGGTVVI
                     KAQVKTGGRGKAGGVKLARTPDEAQQHAAAILNMDIKGHTVHRVLVTVASDIVEEYYL
                     SFLLDRANRTFLAMASVEGGMDIEEVAATKPEALAKVPVDATSGVDAAKASEIVRAAR
                     FPAEVAEAVADTVVKLWKAFVAEDATLVEV"
BASE COUNT          282 a          487 c          568 g          247 t
ORIGIN      
        1 ctgcggtcgg gctgtcgatt gtctgacccg cggcaacgaa tgcaccagcg gcgacggctg
       61 ctacgaccac cctgccgcgc acggcaggcg acggtgctgt caagcggtgc gctgcccggg
      121 taaccgaggg gccaggacgt agcgccgcct cttgtaccgg gtgccgttgg ccgcctgggg
      181 agcggtgtcg agccaagacc tgccttccgt tcggaagttt gtactggcgc cggaggggga
      241 accccgagga agtagcgggt gacccgttcc gcagccagcc gtaatcggac tgtgaccgtg
      301 gagcgggggg aaggtaacga agcgcgacgc cgacgggcaa gtcgccgttc cgcaatacgc
      361 ccggctgtat cggccagcgt tggttctcgc gcgccgtgcg gtcgatccac ccgtccgctc
      421 gtggagatgt gggcggttgg gctggagcac aacgacccgg tcagcggcag cccgacgggt
      481 gctggtaggc ctaaccggaa gctagccttg ggaaaatgag cggacgtatc cgggtggtcg
      541 tcgccaaacc cggacttgac gggcacgacc ggggcgccaa ggtcgtcgca cgtgccctgc
      601 gcgatgccgg tatggaggtc atctacaccg gactgcacca gaccccggaa cagatcgtgg
      661 agaccgcgct gcaggaggac gccgacgcgg tagggctgtc ggtcctctcg ggtgcgcaca
      721 tgacgctgtt cgctcgggtc atcgagttgc tgcagagcaa gggtgcggcc gacgtactgg
      781 tcttcggtgg gggcatcatt cccgatgaag acattccgaa gctcaccgaa ctcggggtgg
      841 ccaaagtatt caccccgggc gccaccacac acgagatcat caactgggtg cgggcggcct
      901 tggccactcc ggtggagtga tctctttcac tggtgccagc gggtcgctgc cagccgcgac
      961 tggatagggt cgggcggtcc gtcggctggc cgagtcacaa tcctggagac ctgcgtggac
     1021 ctgtacgagt accaggcgaa ggatctgttc caagcccacg gggtcccggt actgcccggc
     1081 cgcgtcgcca ccaccagtgc gcaggcccga gcctgcgctg aggagctggg cggcaccgtt
     1141 gtcatcaaag ctcaggtgaa aacaggaggc cgcggcaagg caggtggtgt gaagctggcc
     1201 aggacaccgg acgaggcgca acagcatgcg gccgccatcc tgaacatgga catcaaaggt
     1261 cacaccgtgc accgcgtgct ggtcacggtc gcatcggaca tcgtcgagga gtactacctc
     1321 tctttcctgc tcgaccgcgc caaccgcacc ttcctcgcaa tggccagcgt cgagggcggg
     1381 atggacatcg aggaagtagc ggcgacgaag cccgaagcgc tagccaaggt gccggtggac
     1441 gcaacgtccg gggtggacgc agcgaaggcc tccgagatcg tccgagctgc cagattcccg
     1501 gcggaggtgg ccgaggcggt ggccgacacc gtggtgaagc tgtggaaggc cttcgttgcc
     1561 gaggacgcga ccctggtgga ggtg
//
LOCUS       DATADN010000011         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1358316, whole genome shotgun sequence.
ACCESSION   DATADN010000011 DATADN010000000
VERSION     DATADN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358316"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VHH53_00115"
     CDS             complement(<1..332)
                     /locus_tag="VHH53_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX2288623.1"
                     /translation="MTETSQRYARLADAFAAKIAAVPDDRWASPSPCEGWTARDVVRH
                     VIDSQEMFLGMVGRTLGDIPSVEDDPLGAWTAARSVVQRDLEDAERASAQFEGFAGST
                     TFETAVEH"
     gene            488..>2197
                     /locus_tag="VHH53_00120"
     CDS             488..>2197
                     /locus_tag="VHH53_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX2288624.1"
                     /translation="MDSALEELAAKHGVATWYRDAQRRRVDVDADVVRRVLALLGVGA
                     DTSAQVRDSLAASRKPELPGTVVLRQGQSREIGDSGVLSDEHGTEMPITGTLPADLAP
                     GWYSLTTGEAHSTVLVAPPTLPEPPRTWGWMLQLYALRSARSWGIGDLGDLREFIAWT
                     ATTHGGGAVLVNPLHAVTPTHPVQPSPYSPSSRRFVNPLYLRICDIDAYRRADPALRA
                     KVDALWVRSGGAEPEGERIDYDAVWRAKRAALELLWQAEGRPCPMMSDGLRDFTTFCA
                     LAERHGPRWSRWPSELRHPATANADPQRVAFHAWVQQRCTEQLAAARNAARGMAIGVV
                     HDLAVGVDPEGADAWALQDVLAIGVTVGAPPDIFNQQGQDWGLPPWRPDRLAATGYAA
                     YRDMLRAVLAHADGLRIDHVAGLWRVWWVPPGASPAQGTYVHYDATAMLAALALEAHR
                     TGAVVVGEDLGTVEPEVTEALAAQRMLGCMVLWFARDEDATGQPLLAPRQWPQRAAAS
                     ISTHDLPTAVGFLRGEHVRARAALGLLNDEAAEWAQARTERAELVGLLRGEGLLEDGE
                     AEDD"
BASE COUNT          318 a          735 c          802 g          342 t
ORIGIN      
        1 aagtgctcga cggctgtttc gaacgttgtc gacccggcga atccttcgaa ctgagccgat
       61 gcccgttccg catcctccag gtctcgctga accacggacc gggctgctgt ccacgctcca
      121 agcgggtcgt cctcgaccga cgggatgtcg cccaacgtac gtcccaccat gccgaggaac
      181 atttcctggg agtcgatcac gtggcgcacc acgtcacggg cggtccagcc ttcgcacggc
      241 gacggacttg cccaccggtc gtcgggaact gcggcgatct tcgccgcgaa ggcgtccgcc
      301 aacctcgcgt aacgctgtga tgtctcggtc atgcctgtct cccgcgggta gcgcctgcgg
      361 cggtcgggtc gagatcaggt gatcgagcgt aagggaagtc accacagcgc tcggcccagc
      421 ggggcagtag gcgacgtccg accagggcgc agagcgtcac cggccaagca aggtcagaat
      481 ggcaggcatg gactctgcac ttgaagagct tgcggctaag cacggcgtcg cgacctggta
      541 ccgggacgcc cagcgccggc gggtggacgt cgacgctgac gtggtacgcc gggtgctcgc
      601 gctgctgggc gtcggtgctg atacttcggc gcaggtccgc gactcgctgg cggcatcccg
      661 gaaacccgag ctgccgggca ccgtggtgct gcgtcagggc cagagccggg aaattggcga
      721 ttccggtgta ttaagtgatg agcacggcac cgaaatgccg atcacgggga cgctgccggc
      781 cgatcttgcg cccggttggt actcgctgac cacgggggag gcgcacagca ccgtgctggt
      841 ggccccgccg acgctgcccg agccgccgcg cacctggggc tggatgctgc agctctacgc
      901 ccttcgctcg gcacgatcgt ggggcatcgg ggatctcggt gacttgcgcg agttcatcgc
      961 ctggacggcg accacgcatg gcgggggcgc cgtgctggtc aatccgctgc acgcggtgac
     1021 tccgacgcat ccggttcagc cgtcgccgta cagcccgtcc agccgccgct tcgtcaaccc
     1081 gctgtacctg cgtatctgcg acatcgatgc ctaccgccgc gcggatccgg cgttacgagc
     1141 caaggtggac gccctgtggg tccgatctgg gggagccgag ccggaaggcg agcggatcga
     1201 ctacgacgcc gtctggcggg ccaagcgggc cgcgttggag ctgctctggc aggccgaggg
     1261 aaggccgtgc ccgatgatgt cggacgggct gcgcgacttc accaccttct gcgcactcgc
     1321 tgagcggcac ggcccacgct ggtcgcgctg gcccagcgag ctgcggcacc cggcgacggc
     1381 gaacgcggac ccgcagcgag tggcctttca cgcctgggtg cagcagcggt gcaccgagca
     1441 gctggccgcc gcccgcaacg ctgcccgcgg gatggccatc ggggtggtgc acgaccttgc
     1501 ggtcggcgtg gaccccgaag gcgccgacgc ctgggcgctg caggacgtgc tggccatcgg
     1561 cgtgaccgtc ggtgccccgc cggatatttt caatcagcag ggccaggact gggggttgcc
     1621 accgtggcgc ccggaccggc tggctgccac cgggtacgcc gcctaccgcg acatgctgcg
     1681 cgccgtgctc gcgcatgccg atggattgcg catcgatcat gtcgccggcc tgtggcgggt
     1741 gtggtgggtg cccccgggtg cgtcccccgc gcagggcacc tacgtgcatt acgacgccac
     1801 ggcgatgctt gccgcgctgg cgctggaggc gcaccgcacc ggcgcggtgg tggtgggtga
     1861 ggacctcggc acggtggagc ccgaggttac ggaggcgctg gcggcgcagc ggatgctggg
     1921 ctgcatggtg ctgtggttcg cccgtgatga ggatgccacg ggccagccgt tgctcgctcc
     1981 gcggcagtgg ccgcaacgtg cggcagccag tatctccact cacgacctgc ccactgcggt
     2041 cggctttcta cgcggggagc atgtgcgggc gcgcgcggcg ctgggactgc tcaacgacga
     2101 agccgcggag tgggcccagg cgcgaaccga acgcgcggaa ctcgtcggtc tcttgcgtgg
     2161 cgagggcctg ctcgaggacg gcgaggccga ggacgac
//
LOCUS       DATADN010000012         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1056837, whole genome shotgun sequence.
ACCESSION   DATADN010000012 DATADN010000000
VERSION     DATADN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056837"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VHH53_00125"
     CDS             <1..202
                     /locus_tag="VHH53_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288625.1"
                     /translation="ESTGRADTIRGDHAHCCTFLEITLRLGTDPAALAGACHRCDCRI
                     DDMFDCPASSLGWVPTQRAPSD"
     gene            233..835
                     /locus_tag="VHH53_00130"
     CDS             233..835
                     /locus_tag="VHH53_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2288626.1"
                     /translation="MRVNSRTVTTTARRAQIVDAAITTIAELGLNQASFARIAERAGL
                     SSTRLISYHFAGKDDLMEAVVDEVFSTAGQFIAPFVLAEPTPSAKLRGFIRGSARFYT
                     EHRQHVVAVRDVWANFRRPDGSQRFGMETHEPEFEVVSQIFREGQARGEFREFDPRVM
                     AITLRQALDGLAVLVATDPDLDVDAYTGELVALFDRATRA"
     gene            832..>1878
                     /gene="nuoH"
                     /locus_tag="VHH53_00135"
     CDS             832..>1878
                     /gene="nuoH"
                     /locus_tag="VHH53_00135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX2288627.1"
                     /translation="MTRVQQLLADDPLWLILLKVVALFVLGLLMTMFMIVLERKVVGR
                     MQQRPGPNRVGPGGWLQSLADALKLAFKEDIMPVNADKRVFFIAPVISTIPAFVAFSV
                     IPFGGEVSIFGEPTVLQLVDLPVGVLVVLACSSIGVYGIVLGGWASGSPYPLLAALRS
                     AAQVISYEIALGLSIVGVILYAGSLSTADIVASQSAGWYLLLLAPSFVVFVISMVGET
                     NRAPFDLPEAESELVGGFHTEYSSLKFALFFLAEYVNMVTVSAMATTLFLGGGMWPWP
                     LSFLNGNGWLQLVAFLIKTFVFLFVFIWLRGTLPRLRYDQFMRLGWKVLVPGSLLWIL
                     AIFAIRTWRTSGGQV"
BASE COUNT          242 a          630 c          659 g          347 t
ORIGIN      
        1 cgagtccacg ggtcgcgccg acacgatccg cggcgaccac gcccactgct gcaccttcct
       61 cgagatcacc ctccgcctgg gcacggaccc cgccgccctc gcgggcgcct gccaccgctg
      121 cgactgccgg atcgacgaca tgttcgactg ccccgcgtcc tccctcggct gggtgccgac
      181 ccagagagca ccgtccgact gaccctttac tcagccgggg taatgtttac tcgtgcgagt
      241 aaacagccga accgtcacga ccaccgcccg gcgcgcgcag atcgtcgacg ccgcgatcac
      301 cacgatcgcc gagctggggc tgaaccaggc gtcgttcgcc cggatcgccg agcgggcggg
      361 cctgtcgagc acccggctga tctcgtacca cttcgcgggc aaggacgacc tcatggaggc
      421 cgtcgtcgac gaggtgttca gcaccgccgg gcagttcatc gccccgttcg tcctcgccga
      481 gccgacgccg tcggccaagc tgcgcgggtt catccggggc agcgcgcgct tctacaccga
      541 acaccgccag cacgtcgtcg cggtgcgcga cgtctgggcc aacttccgcc gccccgacgg
      601 cagccagcgg ttcgggatgg agacccacga gcccgagttc gaggtggtct cccagatctt
      661 ccgggagggg caggcccgcg gcgagttccg ggagttcgac ccgcgggtca tggcgatcac
      721 gctccgccag gcgctggacg gcctggccgt cctcgtcgcc accgacccgg atctcgacgt
      781 cgacgcctac acgggcgagc tcgtggcgct cttcgaccgg gcgacgcggg catgacccgc
      841 gtccagcagc tgctggcgga cgacccgctg tggctgatcc tgctcaaggt cgtggccctg
      901 ttcgtgctcg gcctgctcat gacgatgttc atgatcgtgc tggagcgcaa ggtggtgggc
      961 cggatgcagc agcgtcccgg cccgaaccgc gtgggcccgg gcggctggct gcagagcctc
     1021 gccgacgccc tcaagctggc cttcaaggaa gacatcatgc cggtgaacgc cgacaagcgg
     1081 gtgttcttca tcgcgccggt catctccacg atcccggcgt tcgtggcgtt ctcggtgatc
     1141 ccgttcggcg gcgaggtgtc gatcttcggc gagccgacgg tgctgcagct ggtggacctg
     1201 ccggtcgggg tgctggtcgt gctggcgtgc tcctcgatcg gggtgtacgg gatcgtgctg
     1261 ggcggctggg cctccggttc gccgtacccg ctgctggcgg cgctgcgcag tgcggcgcag
     1321 gtgatctcct acgagatcgc gctggggctg tcgatcgtgg gggtgatcct gtacgccggg
     1381 tcgctgtcca cggcggacat cgtggcctcg cagtcggcgg gctggtacct gctgttgctg
     1441 gcgcccagtt tcgtggtgtt cgtgatctcg atggtggggg agaccaaccg ggccccgttc
     1501 gacctgccgg aggcggagtc ggagctggtg ggtgggttcc acaccgagta ctcgtcgttg
     1561 aagttcgcgc tgttcttcct ggccgagtac gtgaacatgg tgacggtctc ggcgatggcc
     1621 accacgctgt tcctgggtgg cgggatgtgg ccgtggccgc tgtcgttcct caacggcaac
     1681 ggctggttgc agttagtggc gttcctgatc aagacgttcg tgttcctgtt cgtgttcatc
     1741 tggttgcggg gcacgctgcc gcggttgcgc tacgaccagt tcatgcggct gggctggaag
     1801 gtcctggtgc cgggcagcct gctgtggatc ctggcgatct tcgcgatccg cacctggcgg
     1861 accagcggtg ggcaggtc
//
LOCUS       DATADN010000013         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_906056, whole genome shotgun sequence.
ACCESSION   DATADN010000013 DATADN010000000
VERSION     DATADN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906056"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VHH53_00140"
     CDS             <1..1381
                     /locus_tag="VHH53_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX2288628.1"
                     /translation="DSVLDVIPVDLVVNAILAAAARSPVTAPAYLHVSSGASNPLTFQ
                     NMYMLCREYFDNNPIPVADRGHVDPPSWRFPGSQQVELMLRAGERAAALAERVLLALP
                     PSKRSQDWLTNLGRHQQDLVQLREYADLYGVYARAEVIYDDRRLRELHASLPAQRGTE
                     HGFDPTAIDWRAYLQDVHCPSITATVRRAGQRRAVPSVDGRAGRQSLPLPERTDVAAV
                     FDLEGTIVASNVVESYLWARLATLPRSRWLGELADLARCAPRYLYAERRDRGELLRAF
                     LRRYAGVRADELRALATEVLGDALLHRVMPEATRQIRRHREAGHRTVLITGAVDLHVE
                     PLSVLFDEVVASRMHATDGMLTGFLETPPMVDEARAAWLQQYAQRHGLQLDRSYAYAD
                     SYSDRPLLEAVGHPHAVNPDAQLFRHARRQRWVVHRWGAHTGGLLEVMLDVTRADTTA
                     GARTRGAHR"
     gene            1378..2583
                     /locus_tag="VHH53_00145"
     CDS             1378..2583
                     /locus_tag="VHH53_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2288629.1"
                     /translation="MNLTLQYHRSAPRYFAARAVSSTRAGSRLAGAVAANMAPLRLVE
                     QADPDPPGESWSRVEPVLSGICGSDLGLLTGRSSPYLGPLTSMPFVPGHEVIGRTLDE
                     LPGLPRGSRVVIDPVLRCAVRGVPACPACITGHTSRCDHITTGRLSAGLQTGFCADTG
                     GGWSRRMLAHASQLHPVPEALPDEIAVLVEPLACAIHAVRRVPINPGASVLVVGAGTV
                     GLLTLLALRKLTAAGEVHVIAKHRHQADRALALGATHIVDSDGTARVLRRSTGALLVE
                     PEFGAGYLLGGMDVAFECTGAAAGLDTALRSLRAGGTVIASGMPSGGVDLTPLWYREL
                     HLVGAYASAVGDEPDVGDFPEAIALAGSAPLDGYVDTKYPLERWRDALDHATAAGRLG
                     TVKVVFAPS"
     gene            2623..>3019
                     /locus_tag="VHH53_00150"
     CDS             2623..>3019
                     /locus_tag="VHH53_00150"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX2288630.1"
                     /translation="MGRPGFVLDVDERTPPLLMHEGEGFRLERLPLGSRVIYPPESLP
                     GIVDLEGTIRHALAHPHGSDPLPELLRPGMRLTIVFDDLSLPLPPMRTPDVRQRVIEQ
                     VLEMAAAAGVDDVELIAANSLHRRMTPAEL"
BASE COUNT          453 a         1056 c         1032 g          478 t
ORIGIN      
        1 cgactccgtg ctcgacgtca tccccgtcga cctggtggtc aacgccatcc ttgcggcggc
       61 ggcgcggtcg cctgtcaccg cgccggcgta cctgcacgtc agctccgggg caagcaaccc
      121 gctgaccttt cagaacatgt acatgctgtg ccgggagtac ttcgacaaca acccgattcc
      181 cgttgctgac cgcggtcatg tcgatccacc gagctggcgg tttccgggca gccagcaggt
      241 ggaattgatg ctgcgcgccg gggagcgagc cgctgcgctc gcggagcggg tgctgctggc
      301 gctgccgcca tcgaagcggt cccaagactg gctgaccaac ctggggcgtc accaacagga
      361 tctggtgcaa ctgcgcgaat acgccgacct ctacggtgtc tacgcccgcg cggaggtgat
      421 ctacgacgac cgccggctgc gggagttgca cgccagcctg ccggcccagc gagggaccga
      481 acacggtttc gatcccactg ccatcgattg gcgggcctac ctgcaggacg tgcactgccc
      541 gtccatcacc gccacggtac gccgggccgg gcagcggcgg gcggtgccga gcgtcgacgg
      601 acgcgctggg cgccagtccc tgccgctacc tgagcgcacc gacgttgccg cggtgttcga
      661 cttggagggc accatcgtgg cctccaatgt cgtcgagtcc tacctgtggg cccggctcgc
      721 cacgctgccc cggtccagat ggctgggtga gctcgctgat ctggctcgtt gcgcgccgcg
      781 ctacctctat gcggagcgcc gagaccgcgg tgagctgctg cgcgcctttc tgcgccgcta
      841 cgccggtgtg cgggccgacg agctgagagc gctggcgacc gaggtgctcg gcgacgcgct
      901 gctgcaccgg gtgatgcccg aggccacccg gcagatccgc cgccaccgcg aggccgggca
      961 tcgaaccgtt ctaatcaccg gcgcagtaga cctacatgtg gaaccactgt cggtgctgtt
     1021 cgatgaggtg gtggccagcc ggatgcacgc cacagacggc atgctgaccg gattcctgga
     1081 gacgccgccc atggtcgacg aagcgcgcgc agcgtggctg cagcagtacg cccaacgcca
     1141 cgggctacag ctggaccgct cctacgccta cgccgacagc tactccgacc gtccgctgct
     1201 ggaggccgtc ggccatccgc atgcggtcaa ccccgacgcc caactgttcc ggcacgcccg
     1261 gcgccagcgt tgggtggtgc accgttgggg agcccacacc ggcgggctcc tcgaggtaat
     1321 gctcgacgtg accagggctg ataccacagc cggtgcccgc accaggggag cgcatcggtg
     1381 aacctgaccc tgcagtacca ccgctcggcg cctcgctact tcgccgcccg cgcggtcagc
     1441 agcacccgcg ccggaagccg gctggcgggt gcggtcgccg ccaacatggc gccactgcgt
     1501 ctggtcgagc aagctgaccc ggacccaccc ggcgagtcct ggagccgggt cgaaccggta
     1561 ctgtctggga tctgcggatc cgatctgggc ctgctcaccg gccggtcctc gccctacctc
     1621 gggccgttga cctcaatgcc tttcgttccc ggccacgagg tgatcgggcg caccctcgac
     1681 gagttacccg gcctgccgcg aggcagccgg gtggtgatcg acccagtgct gcggtgcgcg
     1741 gtacgcgggg taccggcctg cccggcctgc atcacagggc acaccagccg ctgtgaccac
     1801 atcaccaccg gaaggctgtc cgccgggctg cagaccggtt tctgcgccga caccggcgga
     1861 ggctggagcc gccgcatgct cgcgcacgcc agccaactgc atccggtgcc cgaagcgctg
     1921 cccgacgaga tcgcggtgct ggtggaaccc ttggcgtgtg cgatacacgc ggtgcgccgg
     1981 gtaccgatca accccggtgc atcggtgctc gtggtcggtg cgggcaccgt aggcttactc
     2041 accttgctcg cactgcgcaa actcacggca gctggtgagg tgcatgtcat cgccaagcac
     2101 cgccaccagg ccgaccgggc gctggcgctg ggggctaccc acatcgtcga ctccgacggc
     2161 accgccaggg tgctgcgccg cagcaccggg gcactgctgg tcgagcccga attcggcgcg
     2221 ggctacctgc tcggcggcat ggacgtggcc ttcgagtgca ccggggcagc ggcgggactg
     2281 gacaccgcgt tgcggtcgct gcgtgccgga ggcacggtga tcgcatcggg aatgccctca
     2341 ggtggggtgg acctcacccc gctgtggtac cgcgagctgc acctggtcgg ggcctacgcc
     2401 tcggcagtag gcgacgagcc cgacgtgggg gatttccccg aagctatcgc gctggcggga
     2461 tcggcaccgc tggacggtta cgttgatacc aagtacccgc tggaacgctg gcgtgacgcg
     2521 ctcgatcacg ccaccgcggc tggccggctg ggcacggtca aggtcgtctt tgcgcccagc
     2581 tgaggttaag cgcaatgccg cggacaggag caaggtgaga gtgtgggtag accaggtttc
     2641 gtgctcgacg tcgacgagcg gactcccccg ctgctgatgc acgagggtga gggattccgg
     2701 ctggagcgcc tgccgctggg cagccgtgtg atctacccgc cggaatcgct gcctggcatc
     2761 gtcgacctcg aaggcacgat ccgtcacgcc ttggcacacc cgcacggcag cgacccgctg
     2821 cccgagttgc tgcgccccgg gatgcgtctg accatcgtgt tcgacgacct gtcgttgccg
     2881 ctgccgccga tgcggacccc ggatgtccgc cagcgagtga tcgagcaagt tttggaaatg
     2941 gccgctgccg ccggtgtgga cgacgtcgaa ctcatcgccg ccaattcgct gcaccggcgg
     3001 atgactccgg cggagttgc
//
LOCUS       DATADN010000014         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_152226, whole genome shotgun sequence.
ACCESSION   DATADN010000014 DATADN010000000
VERSION     DATADN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152226"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VHH53_00155"
     CDS             <1..485
                     /locus_tag="VHH53_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavoprotein oxidoreductase"
                     /protein_id="HEX2288631.1"
                     /translation="RPAFVPLGPAGNKTGRVAGTVAAGGRARFRGVVGTAVAKVFDLG
                     VARTGLTLAEAQADFGPGAAAIDIVGRSRAKYYPGAEPVTIRLVHEAGGRLLGAQMVS
                     AGDVAKRIDVVAAGLQAGFDLDDLLALDLSYAPPYAPVYEPLLLAAQAAATSVAAVAV
                     "
     gene            489..1214
                     /locus_tag="VHH53_00160"
     CDS             489..1214
                     /locus_tag="VHH53_00160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2288632.1"
                     /translation="MPGQQRVAVVTGGASGIGRATAARLVDDGFRLAVVDLSPDPGSP
                     VVAMKADVSDAIDVERAIHEVLTEFGRIDVLVNNAGISGSADATRCHETPIEEWDRVF
                     AVNVRGPFLCTRTVLPVMVHQGGGHVITVASVAGMVAFPGRCAYTASKGAALQFAKSV
                     AVDYATAGIRSNAVCPGMVATPMTQWRLDIPDLRAAVESTIPIGRVAAAEEIADAISV
                     LASHRLSYLTGAAFVIDGGWTAL"
     gene            1249..2022
                     /locus_tag="VHH53_00165"
     CDS             1249..2022
                     /locus_tag="VHH53_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2288633.1"
                     /translation="MTGQLEGKVALITGAGSGIGRAAAQRFAAEGCAVAVVDMAASAA
                     EETAGKIVADGGRAIACPANVTVDAEVRDAVARTVDTFGSLDVLYNNAGVNSSGSVAD
                     ATEEDWDRCFAVNVKGTFLSSRAAVAHLAAGGSGAIVNQASVAALVGVPNFAAYCAAK
                     GAVVALTRSMAIDLAPRGIRVNAICPGTVFTPLMEPMLRARGDGDMEVGLARTVVKYP
                     IGRLGTPEEIASAALFLASAGASFLTGSILAADGGMTAQ"
BASE COUNT          292 a          655 c          756 g          321 t
ORIGIN      
        1 gcaggccggc cttcgtgccg ctggggccgg cggggaacaa gaccggccgg gtagccggca
       61 cggtggccgc cggcggacga gcccgatttc ggggtgtggt gggaaccgcg gtggccaagg
      121 tcttcgatct cggggtggcc cgcaccggcc tcacattggc ggaggcacaa gccgacttcg
      181 gccctggtgc cgccgcgatc gacatcgtcg ggcgctcccg ggccaagtac tacccgggcg
      241 cggagccggt gacgatccga ttggtgcacg aagccggcgg ccggctgctc ggcgcgcaga
      301 tggtctcggc cggcgatgtc gcaaaacgga tcgatgtcgt ggccgccggc ttacaggccg
      361 gcttcgacct cgacgacctg ctggcgttgg acctctccta cgccccgccg tacgcgccgg
      421 tgtacgagcc gctgctgctc gccgcgcagg cggcggccac cagcgttgcc gcggtggcgg
      481 tgtgagcgat gccaggccag cagcgggtgg cggtggtcac ggggggtgcc tcgggcatcg
      541 gccgggccac ggccgcccgg ctggtggatg acggtttccg gctcgcggtg gtcgacctat
      601 cgccagaccc tggctctccg gtggttgcca tgaaggccga cgtgtcagac gcgatcgacg
      661 tcgaacgcgc gatacacgag gtcctcaccg agttcggccg gatcgacgtg ctggtcaaca
      721 acgccgggat cagcgggtct gccgacgcca cgaggtgcca cgagacaccg atcgaggagt
      781 gggatcgggt attcgcagtc aacgtccgcg gtccgttcct gtgcacccgc accgtgttac
      841 cggtgatggt gcaccagggt ggcgggcacg tgatcacggt ggcatcggtc gccgggatgg
      901 tggccttccc cgggcgctgc gcctacaccg cgtccaaagg tgccgcgctg cagttcgcca
      961 agtcggtcgc ggtggactat gccactgccg gcattcgctc caacgcggtg tgccccggca
     1021 tggtggcgac cccgatgacg cagtggcggc tggacattcc cgatctgcgg gccgccgtcg
     1081 aatccacgat cccgatcggg cgggtcgctg cggccgagga gatcgcggat gcgatctcgg
     1141 tgctggcctc gcaccggctg agctacctca ccggcgccgc attcgtgatc gacggcggtt
     1201 ggactgcact gtgactaccg agctggggac gaggaaccag gagtagccat gaccggacag
     1261 ttggagggca aggtcgcgct gatcaccggg gctggctcgg ggatcggtcg agctgcggcg
     1321 caacgcttcg ccgccgaggg ttgcgcggtc gccgtggttg acatggcagc cagtgccgcc
     1381 gaggaaacag cgggcaagat cgttgctgac ggcggccggg caatcgcctg tcccgccaat
     1441 gtcacagtcg atgccgaagt gcgcgacgcg gtcgcgcgca ccgtcgacac gttcggaagc
     1501 ctcgacgtgc tctacaacaa cgctggcgtg aacagcagcg gctcggtcgc cgatgccacc
     1561 gaagaggact gggaccgctg cttcgccgtg aacgtcaagg gcacgtttct gtcctcgcgc
     1621 gcggccgttg cacacctggc tgcgggcggg tccggcgcga tcgtcaacca agcctcggtc
     1681 gctgcgctgg tgggggtgcc gaacttcgct gcctactgcg ccgccaaggg tgcggtggtg
     1741 gcgctgaccc gctcgatggc gatcgacctc gcaccgaggg gaatccgggt gaacgcaatc
     1801 tgcccgggca ccgtgttcac cccgctgatg gagccgatgc tgcgggcccg tggagacggg
     1861 gacatggagg tggggttggc caggacggtg gtcaagtacc cgatcggccg gctgggcact
     1921 ccggaggaga tcgcctcggc ggcgctgttc ctggccagtg ctggtgcctc gttcctgacc
     1981 ggatcgatcc tggccgccga cggcggcatg acggcgcagt gagg
//
LOCUS       DATADN010000015         2475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1057275, whole genome shotgun sequence.
ACCESSION   DATADN010000015 DATADN010000000
VERSION     DATADN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057275"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VHH53_00170"
     CDS             <1..253
                     /locus_tag="VHH53_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX2288634.1"
                     /translation="MAEEEREGLTDFRRHAGLQVRHDLAVAFAAFEATHFSGVTPVDK
                     DPESYVREVEEEIAASNDQGNDSATKSDGSLAIGSLKRQ"
     gene            complement(580..822)
                     /locus_tag="VHH53_00175"
     CDS             complement(580..822)
                     /locus_tag="VHH53_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288635.1"
                     /translation="MDEFLGIYLNDQLAAGMLWREMARRSQRSNSGTELGEALARVSA
                     EIAEDVETFQRIMRRLGIRLILSRSGWQSVPSAWGG"
     gene            943..1890
                     /locus_tag="VHH53_00180"
     CDS             943..1890
                     /locus_tag="VHH53_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288636.1"
                     /translation="MLEWLGHEHRRARPALLPGERQRHREDLLAADTLSPVQDEEAGS
                     SQDATGLEGALARVERETEACSKAVASLARELKRAHAAAASGQVRELRKALDTVQAMA
                     GDIVERVGAAATAYDVDEVGLLACGAYAKELLAAAEAADVAMFPDDDRLLCYPSIVRV
                     LPGDLALEIDRKRARGIRPSVVVAQLAKAQRAGPRFKPAPFLASLIGAYDLVVAGQGK
                     APGAVVRLLDVYNALTLLPGQSRDYTRAEFARDLYLLDQSGETFSTGGRQLRWAASSG
                     TRQSGVLTTVAMSGQQQRYWGIAVEPGPNKEGTSEEGTG"
     gene            1887..>2475
                     /locus_tag="VHH53_00185"
     CDS             1887..>2475
                     /locus_tag="VHH53_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288637.1"
                     /translation="MNEIRGGLPAGGIGLGPEEYAEFVSKEFLGDYVRAGGAAVRFVV
                     VGSDEVAVRWHRSLASIADADGYLCVEVDAADHRVHLIDQLFAAVARQVDWRDLARRQ
                     VRAGWESLGLPPPGPGKLAVATVAAHHDVAVPEAARSMRRHLESVLLRDPSLDREFRL
                     AVLRLCQCELGTGGVLDAERDAVLAWLRVEPVALRM"
BASE COUNT          392 a          842 c          871 g          370 t
ORIGIN      
        1 catggccgaa gaagagcgcg agggcctcac agacttccgt cgccacgccg gactccaggt
       61 gcgccacgac ctggccgtcg ctttcgccgc attcgaggcc acgcacttct ctggcgtcac
      121 accggtggac aaggaccccg aaagctacgt ccgcgaggtc gaagaggaga tcgcggcatc
      181 caacgaccag ggcaacgaca gcgccaccaa gtcggacgga tcactggcca tcggtagtct
      241 gaagagacag tgacgacgag ccgctgaact tcggtactga cccagcggag agtagtgact
      301 acgtccttcg ctcagggcgc agtttttgct gagccctcct gagctgaagc ggcaagcgct
      361 tcagttcccg cctgcacacg gaagggctca aggtcggcac gttggcgctc agcccgctcg
      421 atcaggtggt cgaagtcaac gtcaggcaag cgggaagcga gaccggcgag gtcgcgcaag
      481 gtcttccaga gcagcttctt gccctcgatc ccatcgcgag aaactccagc tccacgaatc
      541 ggctgagcgg cgagtacgtt cccagccggc cgttgagctt cagccgcccc aggcgctcgg
      601 caccgactgc caacccgatc ttgacaggat cagccggatc ccaagacgac gcatgatcct
      661 ctggaatgtc tcgacatcct ccgcgatttc cgccgagaca cgcgcgagcg cttcaccgag
      721 ctcagtgccg ctattgctgc gctgcgaccg ccgggccatc tctcgccaca gcatgcctgc
      781 tgccagctgg tcattcaggt agatgccgag aaactcgtcc acgcttagga ggtaccccac
      841 tcaccagcac gggaatcccc cgctcgcggc cagtcgggcc gcacggacta ccacccccag
      901 cgcgcccgcc gctgcccacg gtcccctgtt aagcggccgg ccgtgctcga atggctggga
      961 catgagcatc ggcgagcgag gccagcactg ctccccggcg aaaggcagcg ccaccgcgag
     1021 gacctgctcg cggccgatac cctgtcgccc gtgcaggacg aggaagcagg cagcagccaa
     1081 gacgcgaccg gcctggaggg cgcgctcgcc agggtggagc gtgagacgga ggcctgcagc
     1141 aaggccgtcg cctcgttggc tcgcgagctg aagcgcgccc acgctgcggc cgcgtcgggc
     1201 caggtgcggg agttgcgcaa ggccttggac accgtgcagg cgatggccgg tgacatcgtg
     1261 gagcgggtgg gtgcagcggc tactgcctac gacgtcgacg aagtcggcct gctcgcctgc
     1321 ggtgcctacg ccaaggagct gctcgccgcg gccgaggcgg ccgacgtcgc catgttcccc
     1381 gacgacgacc ggttgctgtg ctacccgtcg atcgtgcggg tgttgcccgg cgaccttgcg
     1441 ttggagatcg accgtaagcg agcccgcggg atccgaccgt cggtcgtggt cgcccagctc
     1501 gccaaggcac agcgggccgg gccccggttc aagccggcgc cgtttttggc aagcctgatc
     1561 ggcgcctacg atctcgtcgt cgccgggcag ggcaaggcgc cgggcgcggt ggtgcggctg
     1621 ctcgacgtct acaacgcgtt gacgctgctg cccgggcagt cccgtgacta cacccgcgcc
     1681 gagttcgccc gtgatctgta cctgctcgac cagagcggtg agaccttcag taccggcggg
     1741 cggcagctgc gctgggcggc cagctcgggc acccgccagt ccggtgtgct caccacggtg
     1801 gccatgagcg ggcagcaaca gcggtactgg ggcatcgccg tcgagccggg ccccaacaaa
     1861 gagggcacca gcgaggaggg caccgggtga acgagatccg tggcggcctg cccgcgggcg
     1921 ggatcgggtt ggggcccgag gagtacgccg agttcgtgtc caaggagttc ctgggcgact
     1981 acgtccgcgc cggcggggca gcggtgcgct tcgtcgtggt tggcagcgac gaggtcgcgg
     2041 tgcgctggca tcgcagcctg gcgtccatcg cggacgccga tggctacctg tgcgtggagg
     2101 tggacgcggc ggatcaccgg gtgcacctga tcgaccagct cttcgccgcc gtcgcccgcc
     2161 aggtcgactg gcgcgatctc gcgcgccgcc aggtgcgcgc cgggtgggaa tcgctgggcc
     2221 tgccacctcc tgggcccggc aagctggcgg tggccacggt cgccgcgcac cacgacgtcg
     2281 ccgtgccgga ggctgctcgg agcatgcgcc ggcacctgga gtcggtgttg ctgcgcgacc
     2341 cgtcgctgga ccgggagttc cggctggcgg tgttgcggct gtgccaatgc gagctgggca
     2401 ccgggggcgt cctggacgcc gaacgtgacg cggtgctggc ctggctgcgg gtcgagccgg
     2461 tcgcgctacg gatgc
//
LOCUS       DATADN010000016         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_152810, whole genome shotgun sequence.
ACCESSION   DATADN010000016 DATADN010000000
VERSION     DATADN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152810"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VHH53_00190"
     CDS             complement(<1..1514)
                     /locus_tag="VHH53_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2288638.1"
                     /translation="MSTALAEITEVGSDDARRPLMTSRNIAWMNLGFFGVLFSFGLTQ
                     SAVNPIFLFLGAEAHDLPILNIAGPITGLLIQPIIGAMSDKTWHPKYGRRKPYIIGGS
                     IVMIAILFLFPLVTALWMAVICLWLVDAGNNTAMEPYRALISDRLRKVQIPKGFLIQS
                     MFTGAGAVLANVSLFIFQQVLPGGGEGGVPTWVFVVFWFGAVCAIVTVGVAMLRTTEV
                     TPTDEELAHLQTQSKGLSATVREIAEAVKVMPIGMHKIGLAFLFQWYAMFIYWQFVSV
                     SVAESVWNAAPDTPGYEEAAGWVGLMNGSYNFVTMLSALFLLPLCHRYGGKKVHAGAL
                     TLAGLSLAWLATIETQWLTLVPMIGLGICWASMVGVPYLMVASMVPRERTGVYMGILN
                     MMIVVPMLIQTLTFGWIFENLLGSRGTNAMVLTGALLGCAALAMLWVNSPKSDEDSSV
                     MPLAGRREITVYDRVVVGSDGSASALYAVARAHEVASAAEARVVVVSAYDPDDAG"
BASE COUNT          307 a          538 c          500 g          248 t
ORIGIN      
        1 ccggcgtcgt ccgggtcgta ggcggagacc acgaccactc gagcttccgc cgcagacgcg
       61 acctcgtgcg cccgggccac ggcgtacagc gcggacgctg acccgtccga accgaccacc
      121 acccggtcgt acaccgttat ctcgcgacgt ccggcgagcg gcatcacgct ggagtcctcg
      181 tcggacttcg gcgagttcac ccacagcatc gccagggccg cgcaaccgag cagcgcgccg
      241 gtgagcacca tcgcgttcgt gcccctgctg cccagcaggt tctcgaagat ccagccgaag
      301 gtgagcgtct ggatgagcat cgggacgacg atcatcatgt tcaagatgcc catgtagacg
      361 ccggtgcgct cccggggcac catgctcgcc accatcaggt agggcacgcc gaccatgctg
      421 gcccagcaga tgccgaggcc gatcatcggg accagagtca gccattgggt ctcgatcgtc
      481 gcgagccacg cgagcgagag gccggccagc gtgagcgcgc cggcgtgcac cttcttgccg
      541 ccgtaacggt ggcacagcgg cagcaggaac aaggccgaca gcatggtgac gaagttgtac
      601 gagccgttca tcaggccgac ccagccggcc gcctcctcgt agccaggagt gtcgggggct
      661 gcgttccaca ccgactcggc gacgctgacg ctgacgaact gccagtagat gaacatcgca
      721 taccactgga aaaggaaagc caggccgatc ttgtgcatgc cgatcggcat caccttcacg
      781 gcctcggcga tctcgcggac cgtggcggag agccccttcg actgcgtctg aaggtgcgcc
      841 agctcctcgt cggtcggagt gacctccgtg gtccgcagca tggcaacgcc gaccgtgacg
      901 atggcacaca ccgcgccgaa ccagaacacc acgaagaccc aggtgggcac ccctccctca
      961 ccgccgccgg ggagcacctg ctggaagatg aacagcgaca cattcgccag taccgccccg
     1021 gcaccggtga acatgctctg gatcaggaag cccttcggaa tctgcacctt ccgcagccgg
     1081 tcggagatca acgcccggta gggctccatc gcggtgttgt tgcccgcatc caccagccac
     1141 aggcagatca cggccatcca cagcgcggtc accagcggga agaggaacaa gatggcgatc
     1201 atcacgatgc tgccgccgat gatgtacggc ttgcgccggc cgtacttcgg atgccacgtc
     1261 ttgtcgctca tcgcgccgat gatcggctgg atcagcaagc cggtgatcgg gccggcgatg
     1321 ttgaggatcg gcaggtcgtg cgcctccgcc cccaggaaca agaagatcgg gttcaccgcg
     1381 ctctgggtca gcccgaagct gaacagcacc ccgaagaacc cgagattcat ccaggcgatg
     1441 ttcctggagg tcatcaaggg tcgacgtgcg tcgtccgagc cgacttccgt gatttccgcc
     1501 agtgctgtgg acatggacag cgctcctctt caagtggtcc aagaagccgg gtgaagttgc
     1561 gttcgtgcgc aagtgcgagg ccgcgttttg acc
//
LOCUS       DATADN010000017         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_605209, whole genome shotgun sequence.
ACCESSION   DATADN010000017 DATADN010000000
VERSION     DATADN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605209"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VHH53_00195"
     CDS             complement(<1..350)
                     /locus_tag="VHH53_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase II"
                     /protein_id="HEX2288639.1"
                     /translation="MGNSGVDSVQCAAATPEHPQPYRELGLADDEYARIREILGRRPT
                     EAELAMYSVMWSEHCSYKSSKVHLRYFGETTTAAMRQRMLAGIGENAGVVEVGDGWAV
                     TFKIESHNHPSYVEP"
     gene            complement(448..1140)
                     /gene="purQ"
                     /locus_tag="VHH53_00200"
     CDS             complement(448..1140)
                     /gene="purQ"
                     /locus_tag="VHH53_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX2288640.1"
                     /translation="MSRASCRIGVITFPGTLDDVDAARAVRRAGAEAVALWHADEDLR
                     GVDAVVVPGGFSYGDYLRAGAIAAHQPVLRALINAASRGTPVLGICNGFQILCEAGLL
                     PGALVRNVGLHFVCRDQRLRVESSATAWTGRYPPGAEILVPLKSIEGRYVADAATLDE
                     LEGDGRVVFRYLGASPNGAANGIAGIASADGRVVGLMPHPEHAIDSLTGPSADGLDLF
                     RSAVDSALGCAL"
     gene            complement(1137..1370)
                     /gene="purS"
                     /locus_tag="VHH53_00205"
     CDS             complement(1137..1370)
                     /gene="purS"
                     /locus_tag="VHH53_00205"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX2288641.1"
                     /translation="MARVVVDVVLKPEILDPQGQAIARALPRLGFDGVHDVRQGKHFE
                     LDVAEAIDDADLHRMAGELLANPVIEEWTVRRL"
BASE COUNT          271 a          551 c          486 g          228 t
ORIGIN      
        1 ggctccacgt aggacgggtg attgtgcgat tcgattttga aggtcactgc ccagccgtcc
       61 ccgacctcga ccacaccagc gttctcgccg ataccggcga gcatccgttg ccgcatcgcc
      121 gcggtggtcg tctccccgaa atagcgcagg tgcaccttgg acgacttgta agagcagtgc
      181 tcgctccaca tcaccgagta catcgccagt tcggcctcgg tcggccggcg cccgaggatc
      241 tcccggatcc gcgcgtactc gtcgtcggcg agcccgagct cccggtaagg ctggggatgc
      301 tcaggagtag cagcagcgca ctgcacagag tcgacaccgg agttgcccac cctctgagag
      361 tattcgtcgc catctggagc cgaatgactg tcaccgacca catgcgaacc gctattcgga
      421 gccgaacggc aatgtgcggc gtccggatta gagggcacag ccgagcgccg agtcgacggc
      481 cgagcggaac aggtccaggc catcagccga tggcccggtg agcgagtcga tcgcatgctc
      541 aggatgcggc atgagcccga ctacccgacc atccgcggag gcgatcccgg cgatgccgtt
      601 cgccgcgcca ttcgggctgg cgccgaggta ccgaaacacc acccggccgt cgccctcaag
      661 ctcgtccaac gtcgccgcat cggccacata ccggccctcg atggacttca gcgggaccag
      721 gatctcggcg cccggcgggt accggcccgt ccaggccgta gcgctgctct ccacccgcag
      781 ccgctgatca cggcagacaa agtgcagccc gacgttgcgg accagagcgc cgggcagcaa
      841 ccccgcctcg cacaggatct ggaaaccgtt gcagatgccc aagaccggcg taccgcggct
      901 cgctgcgttg atcaacgccc gtagcaccgg ctggtgtgca gcgatcgcgc cggcgcgcag
      961 gtagtcacca taggagaacc cgcccggtac cacgacggcg tccacgccgc gcagatcttc
     1021 atcggcatgc cacagcgcta ccgcctcggc accagcgcga cgcaccgcac gggcagcgtc
     1081 gacgtcatca agcgtgccgg ggaaggtgat gacgccgatc cggcaggacg ccctgctcac
     1141 agtcgccgca ccgtccactc ttcgatcacc gggttggcga gcagttcacc cgccatccgg
     1201 tgcaggtccg catcgtcgat ggcttccgcg acgtccagct cgaagtgctt accctgccgc
     1261 acgtcgtgca ccccgtcgaa gcccagccgg ggcagcgcgc gcgcgatcgc ctgaccctgc
     1321 gggtcaagga tctccggttt caagaccacg tccaccacca ctcgtgccac gccgccgagg
     1381 ctaccgggag tccgggaatc catggcgccg cttcggccga ggaggccgag ttgcccgcgg
     1441 cgtggcaggc tgacggctga ccgttgcgag gaggtgacca cccatgcaga tcgtccacct
     1501 ggggcagtcc tgcgtgctga ccgagattgg ggcagc
//
LOCUS       DATADN010000018         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_454651, whole genome shotgun sequence.
ACCESSION   DATADN010000018 DATADN010000000
VERSION     DATADN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454651"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1132
                     /locus_tag="VHH53_00210"
     CDS             386..1132
                     /locus_tag="VHH53_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2288642.1"
                     /translation="MWSQDGSREQVGRADRILAAAGELLLRFGYKRVTIEDIANQAEV
                     GKGTVYLHWNTKQELFAELLLREVTEVTRELVASMRADPTGVLLHRVTSVSFQAIMQR
                     PLARALYTRDLDTLGKLAASETAGVAARQQVAGALFDEYVDLLRAHGLLRTDIDRQAQ
                     NYVLNAVGTGFFVTEQFLPEQLQEISLEHKAAALAEVIRLALEPPGEPDPDTLRSIAP
                     RAIELFDRMCVERDPATATSHSNYRSNDDN"
     gene            1122..2306
                     /locus_tag="VHH53_00215"
     CDS             1122..2306
                     /locus_tag="VHH53_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2288643.1"
                     /translation="MTTELHTPPTIQDGGHTLLPWLREMRDDHPVWRDAYGIWHVFRY
                     ADVHKAISEPQVFSSATARVVPAAQRFSAGNLLQTDPPRHHTLRRLIGAAFSPKVVTG
                     LAPRIADVTRELLDATGDAAEFDLVPALAYPLPVTVIAELLGLPTSDRELFGSWADRL
                     FKQDTADPNDPELARKIDEATADMLAYLGEHCADRRAHPQDDLISRLAAVEVDGERLS
                     DEEVVNFSVVLLLAGHITTTALLGNTVLSVNEHRDVWAQLHADRSLVEPAIEEVLRYR
                     SSFTQVGRATMVETELGGHVIPADVIVTPWLLSANRDEREFTDPDRFDIHRRDKHHLA
                     FGHGIHFCIGQLLARVEARVAVGVLLDRYAEIRLAREAPLEFYERGIFAARNVPVVVR
                     RA"
     gene            complement(2311..>2412)
                     /locus_tag="VHH53_00220"
     CDS             complement(2311..>2412)
                     /locus_tag="VHH53_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX2288644.1"
                     /translation="RMLSDAGGIPAHGNQRSEWDAGCRFDFENPDYR"
BASE COUNT          421 a          797 c          787 g          407 t
ORIGIN      
        1 gggtgcccgt gatgtgttgg accgcgatcg gctggtaggc gtttgatgcg agcgccgctg
       61 ccgagagcaa tccagcgcca atagcgcaga gtagcgccac tgctaccagt accagcagac
      121 gcctcatacc tatttattct cggtactaac cacgcttgga acgttgttcc cgcactttct
      181 cacaaaattt tcaggaaacc tggtttcggg atctaccagc gtcgcagagc gggaaaagcc
      241 caatactgac agtagcgggc cagcaaggct gtccacgcag cctggcccaa cggttcacgt
      301 tgactggtgt cggcggtagg gctagcttat ccgtatgact gatgaccaga tgagtaatcc
      361 agtcaccagt cgagcgaggg ggtcgatgtg gagccaggac gggagccgcg agcaggtcgg
      421 tcgagccgac cgcatcctgg cagcagccgg cgagctgctg ctgcgcttcg gatacaagcg
      481 ggtcaccatc gaggacatcg ccaaccaagc tgaggttggc aagggcaccg tctacctgca
      541 ctggaacacc aagcaggagc tcttcgccga gctgttgctg cgtgaggtca ccgaggtgac
      601 gcgggaactc gtcgccagca tgcgggccga cccaaccgga gtgctgctgc accgggtgac
      661 gagtgtgagc tttcaggcga tcatgcaacg gccgctggcc agggcgctgt acacccgcga
      721 cctcgacact ctggggaagc tggccgccag cgagacggcc ggcgtggcgg cgcggcagca
      781 agtcgccggc gcgttgttcg acgagtacgt cgatctccta cgggcgcatg gcctgctccg
      841 tacggacatc gaccgccagg ctcaaaacta cgtgctcaac gcggtcggca ccggcttctt
      901 cgtcaccgag cagttcctgc ccgagcagct gcaggagata tctctcgaac acaaggcggc
      961 ggcgcttgcc gaggttatcc ggctggccct ggaaccgccg ggcgagcccg atcccgacac
     1021 cctgcggtcg atcgccccgc gggccatcga gctgtttgat cggatgtgcg tggaacgtga
     1081 cccggccaca gccacatcgc acagcaacta ccggagcaat gatgacaact gagctgcaca
     1141 caccgccgac catccaagac ggtggtcaca ccctgctgcc ctggctgcgc gaaatgcgcg
     1201 atgaccaccc ggtatggcgc gatgcgtacg gcatctggca cgtgttccgc tacgccgacg
     1261 tgcacaaagc gatttccgaa ccgcaagtgt tttcctccgc cacggcccga gtggtcccgg
     1321 cggcgcagcg gttcagcgcc ggcaacctgc tgcagaccga cccgccgcgc caccacacgc
     1381 tgcgccggtt gatcggtgcg gccttcagcc cgaaggtcgt caccgggctg gctccccgca
     1441 tcgccgacgt cacccgcgag ctgctcgacg ccaccggtga tgcagccgag ttcgaccttg
     1501 tccccgcctt ggcctacccg ctaccggtga ccgtgatcgc cgagttgctc ggcctgccga
     1561 cctcggaccg tgagttgttc gggtcctggg cggacaggct gttcaagcag gacacggcgg
     1621 atccgaacga cccggagctg gcccgcaaga tcgacgaggc caccgccgac atgctcgcct
     1681 acctgggcga gcactgcgcc gatcggcggg cccacccgca ggatgacctg atcagccggc
     1741 tggccgccgt ggaggtcgat ggcgagcgcc tgtccgacga ggaggtggtg aacttctcgg
     1801 ttgtgttgct gctcgccggg cacatcacca ccaccgcgtt gctcggcaac accgtgctgt
     1861 ccgtcaatga gcaccgtgac gtgtgggcgc agctgcacgc agaccgttcg ctggtggaac
     1921 ccgccatcga ggaggtgctg cggtaccgct cctcgttcac ccaggttggc cgggcgacca
     1981 tggtcgagac cgagctgggc ggccacgtca tcccagcgga tgtgatcgtc accccttggc
     2041 tgctgtccgc caaccgggac gaacgcgagt tcaccgaccc ggacaggttc gacatccatc
     2101 gccgcgacaa gcaccactta gccttcggtc acggcatcca cttctgcatc ggccagttgc
     2161 tggcccgggt ggaggcccgg gtcgcggtgg gggtgctgct ggaccggtac gccgagatcc
     2221 ggctggcccg cgaggcgccg ttggagttct atgagcgggg aatcttcgct gcccgcaacg
     2281 tgccggtcgt cgtacggcgg gcttgaggcc tcagcggtaa tcggggttct cgaagtcgaa
     2341 ccggcaaccg gcgtcccatt ccgagcgctg gttgccgtgt gcggggatgc cgccggcgtc
     2401 ggagagcatt cg
//
LOCUS       DATADN010000019         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_906945, whole genome shotgun sequence.
ACCESSION   DATADN010000019 DATADN010000000
VERSION     DATADN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906945"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="VHH53_00225"
     CDS             complement(<1..1518)
                     /locus_tag="VHH53_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX2288645.1"
                     /translation="MGEPLLNAGIFLFFVVVTLVIVLRASRSTKTAADYYAAGRSFTG
                     PQNGVAISGDYLSAASFLGIAGAISIYGYDGFLYSIGFLVAWLVALLLVAELLRNTGK
                     FTMGDVLSFRMRRQPVRAAAAVSTLAVSFFYLLAQMAGAGGLVALLLNVQSRGAQALV
                     VTVVGVLMIIYVLVGGMRGTTWVQIVKAALLIIGAGMMTFWVLGAFGLNLSALLGEAA
                     ASSSQGAAVLEPGLQWGLNTTTKIDFVSLAIALVLGTAGLPHILMRFYTVPSAKEARR
                     TVVWAIWLIGVFYLFSLVIGYGAAALVDGGPQRIANMPGEENSAAPLLAYQLGGELLL
                     GFISAVAFATILAVVAGLTITASASFAHDVYANVIKHGDLEPDSEVRVARITACVIGA
                     LAILGGIFALGQNIAFLVALAFAVAASANLPTILYSLFWKRFNTSGVLWSMYGGLVSC
                     VVLIILSPAVSGSTGSMFPGLDFAIFPLSNPALVSVPLSFLLGYLGSVLSKDEADD"
     gene            complement(1587..1940)
                     /locus_tag="VHH53_00230"
     CDS             complement(1587..1940)
                     /locus_tag="VHH53_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEX2288646.1"
                     /translation="MSTTERFGGADAPSPVDPWTTAQQSAEFSTLRRKLRGFIFPMTA
                     FFLSWYFLYVLLAAFAPQFMAIRVLGNINVGLLIGLLQFVTTFAITTAYVRFATKNLD
                     PIGVRIREEIEGVAR"
     gene            complement(2049..3617)
                     /locus_tag="VHH53_00235"
     CDS             complement(2049..3617)
                     /locus_tag="VHH53_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEX2288647.1"
                     /translation="MREQPDLLFIVGAMIAVAAATMVSGLYGARTRSTSDFLVASRTV
                     GPRWNAAAISGEFLSAASFLGIAGLVLKGGVDALWFPVGFTAGYLALLLFVAAPLRRS
                     GAYTLPDFAEERLSSATLRKICTVFVIIIGWLYLVPQLQGAGLTVAVVSNHPQWMGAA
                     VVMVIVTFNIYTGWMRSITVVQAFQYWIKLTALPVPVFVLLIHYAGKNQGVRDSLSAT
                     MDEAWLRPFSSSSSSSLLATYSLVVALMLGTMGLPHVLGRYYTNPDGLAARKTTLVVL
                     ALLGLFYLFPMISGWLARLYVPQLLGTGNTDAALLLLPGAVLDGWPAQLLGALIAAGA
                     FAAFVSAASGLVVSVAGVLSTDVLPGRLRDFRVATLAAWSVPLAFALAVSSLDLAQGV
                     GLAFAVAASSFCPLLVLGIWWRGLTDAGAAAGLLVGGGSAFLAVLPAATGVFRPEWQE
                     VLAQPAVFTVPLAFLTMVVVSRRTRDRIPIDVTRILLRLHAPDRLGLAEDRDIARLGM
                     AATLLRPRSGRHRR"
     gene            complement(3626..4135)
                     /locus_tag="VHH53_00240"
     CDS             complement(3626..4135)
                     /locus_tag="VHH53_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288648.1"
                     /translation="MSPDKGTERDERLNADEPPISSDGASSREDPWAALEKAAQGSSY
                     APAGKAKLGKRERVVLAKRRNSRRVVRTLAELEDQTGVGEVLVNQLVRVQLILAIRLM
                     ALTVAVVGSIPLMLWLAPSLGKISVFGVPLPWLLLGFAVYPFFLAVAWSYNRRVDRNE
                     QDFAEMVEN"
     gene            complement(4132..4950)
                     /locus_tag="VHH53_00245"
     CDS             complement(4132..4950)
                     /locus_tag="VHH53_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX2288649.1"
                     /translation="MSGNDGNPGLVVLAVDDEKGAMALLVEVLDAEPRIGTVLQACNA
                     TEALEALRGEKRIANGTSPPRVDVVFLDIRMPGLDGLELARVLAMIPDPPVVVFVTAY
                     DDKALDAFDIGAADYLMKPVSRERLANTMRRIVQSRHNTAIPVEEQNAEDEVIPVELA
                     GTTKLVPRSSVRWVEAQGDYARLHTGEGNSHLVRIPLSQLEDRWSDAGFVRIHRSFLV
                     ALPLVTELRMSASGYVVRVGNGDDAAELPVSRRHTRELKDRLVRAAKQGWTTSR"
BASE COUNT         1017 a         1755 c         1684 g          756 t
ORIGIN      
        1 gtcgtccgcc tcgtccttgc tcagcacgct gccgaggtag ccgagcagga acgacagtgg
       61 caccgaaacc agggcggggt tgctcagcgg gaagatcgcg aagtccaggc cggggaacat
      121 cgaacccgtg ctgccggaca ccgccggcga caagatgatc agcaccacgc agctgaccag
      181 cccgccgtac atgctccaca acacgccgct ggtgttgaac cgtttccaga acagtgagta
      241 cagaatggtc ggcaggttcg ccgaggcggc caccgcaaaa gccagcgcca ccaagaaggc
      301 aatgttctgg ccgagtgcga agatgccgcc gagaatcgcc agcgcgccga tgacgcaagc
      361 ggtgatgcgg gcgacccgca cctcactatc cggctcgagg tcaccgtgct tgatcacgtt
      421 ggcgtacacg tcgtgcgcaa aggacgccga cgcggtgatc gtcagcccgg cgaccacggc
      481 gaggatggta gcgaaggcca ccgcggagat gaagccgagc agcagctccc cacccaactg
      541 gtaggccagt agcggcgctg ccgagttctc ctcaccgggc atgttggcga tccgctgcgg
      601 tccgccgtcc accagtgccg ctgcgccgta gccgatgacc aacgagaaca ggtagaagac
      661 accgatcagc cagattgccc acaccaccgt acggcgggcc tccttggccg acggcacggt
      721 gtagaagcgc atcaagatgt gcggaagacc cgcggtgccc aacaccagcg cgatcgccaa
      781 cgagacaaag tcaatcttgg ttgtggtgtt gagtccccac tgcaggccgg gttcgagcac
      841 cgcggccccc tgcgatgaac tcgccgcggc ctcgcccagc agcgcagaca ggttgagccc
      901 gaaggcgccc agcacccaga aggtcatcat gccggcaccg atgatcagca gggcggcctt
      961 gacgatctgc acccaggtgg tgccgcgcat tccaccgacc agaacgtaga tgatcatcag
     1021 gacgccgacg actgtgacca cgagcgcctg agcaccgcgg ctctggacgt tgagcagcag
     1081 cgccaccagg ccaccggcgc cggccatctg cgccagcaga tagaaaaacg acaccgcgag
     1141 cgtcgacacc gccgcggcgg cccgcaccgg ttgccgtcgc attcggaagc tcagcacgtc
     1201 gcccatggtg aacttgccgg tgttccgcag cagttcagcg accagcagca gggccactag
     1261 ccaggcgacc aggaagccga tcgaatacag gaagccgtcg tagccgtaga tcgagatggc
     1321 cccggcgatg cccaggaatg acgccgcgga caggtagtca ccggagatcg cgacaccgtt
     1381 ctgcgggccc gtgaatgacc ggcccgcagc gtagtagtcg gcggcggtct tggtgctgcg
     1441 ggaggcccgg agcacgatca ccagggtgac cacgacgaag aacaggaaga tcccggcgtt
     1501 gagtagcggt tcccccaccg tctcctgagc cagccaggcc gcgtctgacg aagctggggc
     1561 cgaggacgtc ggtgtgatca agccggtcac cgggccactc cttcgatctc ctcgcggatc
     1621 cggacgccga tcgggtccag gttcttggtc gcaaaccgaa cgtaggcggt cgtgatcgcg
     1681 aaggtggtga cgaactgcag caggccgatg agcaacccga cgttgatgtt gcccagcacc
     1741 ctgatggcca tgaactgcgg ggcgaacgcc gccagcagga catacaggaa ataccagctc
     1801 aggaagaacg ccgtcatcgg gaagatgaaa ccgcggagct tcctgcgcag ggtggagaac
     1861 tccgcacttt gttgggcggt ggtccagggg tccacaggcg acggggcgtc ggcaccgccg
     1921 aaacgctcgg tggtgctcat gcaaacacct ccaggacagt cagaatgacc attgtgcttc
     1981 gtgtgtggcc agggcaaggg tcaccctagt cgccgacgat gcgcagaacg ttgggccgat
     2041 cggagcagct accggcggtg ccgaccgcta cgcggccgca gcagggtggc tgccatccct
     2101 aagcgggcga tgtcccgatc ctcggccaac cctagccggt ccggcgcatg cagccgcagc
     2161 aggatacgtg ttacgtctat cgggattcga tctctggtcc ggcggctcac caccaccatg
     2221 gtcaggaacg ccagcggcac ggtgaacacc gcaggctggg ccagcacctc ctgccactcg
     2281 ggccggaaca cgccggttgc ggcgggcagc accgcaagga acgccgaccc accgccgacc
     2341 agcaaaccgg ccgccgcgcc ggcgtcggtc agcccgcgcc accagatgcc cagcaccagc
     2401 agcgggcaaa acgatgaggc cgccaccgcg aaagccaatc ccacgccctg ggccaggtcc
     2461 agcgacgaca cggcaagcgc gaaggccaac ggcactgacc aggccgccaa ggtggccacc
     2521 cggaaatccc ggagccggcc gggcaggacg tcggtggaca gcaccccggc gacactgacc
     2581 accaggcccg atgccgcgga cacgaaggcg gcgaaagctc cggcggcgat cagcgcgccg
     2641 agcagttgcg ccggccagcc gtccaatacg gccccgggta gcagcagcag cgcggcgtcg
     2701 gtgttcccgg tgcccagtag ctgcgggacg tagagccggg ccagccagcc ggagatcatc
     2761 gggaacaggt agaacaagcc gagcagcgcc agcaccacca gggtggtctt gcgggcggca
     2821 aggccgtccg gattggtgta gtagcgaccc agcacatgtg gcagccccat ggtgcccagc
     2881 atgagcgcga cgacgagtga gtaggtggcc agcaatgagc tgctgctcga gctgctgaac
     2941 ggccgcagcc aggcctcgtc cattgtggca ctcaatgagt cccgaacgcc ctggttcttc
     3001 ccggcgtagt gaatcagcag cacgaagact ggtacgggca gcgcggttaa tttgatccag
     3061 tactggaagg cttgcacgac ggtgatcgag cgcatccagc cggtgtagat gttgaatgtc
     3121 acgatcacca tgaccacggc cgcgcccatc cactgcggat ggttcgacac gacggcgacg
     3181 gtcagcccgg cgccctgcag ctgtggcacc aaataaagcc aaccgatgat gatcacgaat
     3241 acggtgcaga ttttgcgcaa cgttgccgaa ctcaaccgct cctcggcgaa gtcgggcagg
     3301 gtgtaggccc cggatcggcg cagcggcgcc gccacgaaca gcagcagggc aaggtatccg
     3361 gcggtgaacc caaccgggaa ccacaacgca tccactccgc ccttgagcac tagcccggcg
     3421 ataccgagga aggacgcggc ggacaggaat tccccggaga tcgctgcggc attccagcgc
     3481 ggccccaccg ttcgggaagc gaccaggaaa tctgaggtcg accgggtcct cgctccgtag
     3541 agcccgctga ccatggtcgc cgcggccact gcgatcatcg ccccgacgat gaacaacaag
     3601 tccggctgtt cgcgcacgct tgcgctcagt tctcgaccat ctcggcgaag tcctgctcgt
     3661 tgcggtccac ccggcggttg taggaccaag ccacggcgag gaagaacggg tagaccgcga
     3721 acccgagtag cagccacggc aggggtactc cgaaaacact gatcttgcct agtgacggtg
     3781 ccagccacaa catcagggga atgctgccca ccaccgctac ggtgagcgcc atcagccgga
     3841 tcgccaggat cagctggacc cgtaccagct ggttgaccag aacctcaccg acaccggtct
     3901 ggtcctccaa ctcggcgagg gtacgcacca cccggcggct gttgcgccgc ttcgcgagca
     3961 cgacccgttc ccgcttgccg agcttggcct tgccggcggg tgcgtatgac gacccttggg
     4021 cggctttctc caaggcagcc cacgggtctt cacgcgagct ggccccgtca gagcttatcg
     4081 gcggttcatc ggcgttgagg cgttcatcgc gttcagtgcc tttgtcgggg ctcatcgaga
     4141 cgttgtccag ccctgtttgg ccgcacgcac caagcggtcc ttgagttcac gggtatgccg
     4201 ccggctgact ggcagttcgg cggcatcgtc tccgttgccc acccgcacca catagcccga
     4261 cgctgacatg cgcagttcgg tgaccaacgg cagggcgacc agaaaggagc ggtggatgcg
     4321 cacgaagccg gcatcagacc accggtcctc caactgcgac agcgggatac ggaccaggtg
     4381 gctgttgccc tcgccggtgt gcagccgcgc gtagtcgcct tgcgcttcga cccaacgcac
     4441 cgatgagcgc ggcaccagtt tggtggtgcc ggcgagctca accgggatga cctcgtcctc
     4501 ggcgttctgc tcctcgaccg ggatggctgt gttgtgccgg gactgcacaa ttcgccgcat
     4561 cgtgttggcc agccgttccc ggctcaccgg cttcatcagg tagtcggccg cgccgatgtc
     4621 gaatgcgtcg agcgctttgt cgtcgtaggc ggtgacgaac acgaccaccg gcggatcggg
     4681 gatcatggcg agcacccggg ccagctccag gccgtccagc cccggcatcc ggatgtccag
     4741 gaacacgaca tcaacgcggg gcggcgacgt accgttggcg atccgcttct caccccgcaa
     4801 cgcctctaac gcctcagtcg cgttgcaggc ctgcaggacg gtgccgattc gcggctcggc
     4861 gtcgagcact tcgaccagga gagccatcgc ccccttctcg tcgtccactg cgagcacgac
     4921 aagcccgggg ttgccgtcgt tgccgctcat cgaccgccct tccgacctct ggtccccccg
     4981 gacccaacaa tcctgcacat ggccggtacc gctgtccacg gttggtggtc gctgccgtgg
     5041 tgtggcaaaa cggtgcctga cccgatcaca gaccgaagcg cgaccatgcc gcacggtgtt
     5101 cgagtgcctc cagaaccgct agcgcggagt ccgggccact gtgtccggtg agggagatcg
     5161 ccgcggggcg tagccccagg cgagtcagca gcggccggcg cgcctcgacc at
//
LOCUS       DATADN010000020         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1057824, whole genome shotgun sequence.
ACCESSION   DATADN010000020 DATADN010000000
VERSION     DATADN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057824"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VHH53_00250"
     CDS             <1..1087
                     /locus_tag="VHH53_00250"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX2288650.1"
                     /translation="TIATGSAAAALGGYTAVFAMANTDPVADTAVVVEYVHRRGREVG
                     LVDVHPVGAVTAGLRGERLAELGAMAASAAAVRMFSDDGLCVHDSLLMRRALEYTRGL
                     GGVVAQHAEDPRLTAGAQAHESQIAAQLGLAGWPAAAEESIVARDCLLARDAAAPLHV
                     CHVSTAGTVALLRWAKESGIRVSAEVAPHHLMLTDDRLTGYDPVNKVNPPLRTAADTE
                     ALRSALAEGIVDCVATDHAPHAAQDKDCEWSAARPGMLGLQTALSVVAATMVRPGLLD
                     WRGVARVMSERPARIGGVTDQGRPIAVGQPATLVLVDPDATWTVRGTQLASLAANTPY
                     EGMELPARVVATLLRGEVTTEDGKVQR"
     gene            1084..>1847
                     /locus_tag="VHH53_00255"
     CDS             1084..>1847
                     /locus_tag="VHH53_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEX2288651.1"
                     /translation="MTTAAGRPGTPAVLVLEDGTTVRGASYGAVGQALGEAVFATGMT
                     GYQETLTDPSYRRQIVVQTAPHIGNTGWNDEDDESHRIWVAGYVVRDPARITSNWRSR
                     RSLEQELSAQGVVGISGVDTRALTRRLREHGSMRAGVFSGDALAQPAQLVARVLASPS
                     MVGADLVGEVTAAQPYLVPAGPAQHRFRVVALDLGIKSNTPRMLAARGIETHVLPATA
                     TLEQVLEREPDGVFLSNGPGDPATADHAVSLTRAVLA"
BASE COUNT          254 a          633 c          696 g          264 t
ORIGIN      
        1 gacgatcgcc accggctcag cggccgcggc gctgggcggc tacaccgctg tgttcgcgat
       61 ggcgaacacc gatccggtgg ccgatacggc cgtggtcgtc gagtacgtgc accggcgcgg
      121 gcgcgaagtt gggctggtcg acgtgcatcc cgtcggcgcg gtcaccgctg gactgcgtgg
      181 cgagcggctc gccgagctgg gggcgatggc cgcgtccgct gccgcggtgc ggatgttctc
      241 cgacgacggc ttgtgcgtgc acgactcact gctgatgcgc cgagccctgg agtacacccg
      301 cgggttgggc ggggtggtcg ctcaacacgc cgaggatccc cggctcaccg caggcgccca
      361 ggcgcacgag agccagatcg ccgcccagct ggggctggcg ggctggcccg ccgccgcgga
      421 ggagtccatc gtggcgcggg actgcctgct cgctcgcgac gccgcggcgc cgctgcatgt
      481 ctgccacgtg tccaccgcgg gaacggtcgc actgctgcgc tgggccaagg agagcggaat
      541 ccgggtgtcc gccgaggtcg ccccgcatca cctgatgctc accgatgatc gtctgaccgg
      601 gtacgacccg gtcaacaagg tcaacccgcc gctgcgcacc gctgccgaca ccgaagcgct
      661 gcgcagcgca ctggccgagg ggatcgtgga ctgcgtggca accgaccatg ccccgcacgc
      721 cgcgcaggac aaggactgcg aatggtccgc agcccggccc ggaatgctcg gactgcagac
      781 cgcgctgtca gtggtggcag ccacgatggt gcggcctggc ctgctggact ggcggggagt
      841 ggcgcgggtg atgagcgagc gcccggcccg aatcggtggg gtgaccgatc agggacgtcc
      901 gatcgcggtg gggcagccgg cgacgctggt cctggtggac cccgacgcca cctggaccgt
      961 gcgcggtacg cagctggcca gcctggccgc caacacccct tacgaaggga tggagctgcc
     1021 cgctcgggtg gtggccaccc tgttgcgtgg cgaggtcacg accgaggacg ggaaggtgca
     1081 gcgatgacga cagctgcggg ccggccgggc acaccggccg tgctggtgct ggaagacggc
     1141 accacagtgc gcggcgccag ctacggcgcg gtgggccagg ccctgggtga ggcggtgttc
     1201 gccaccggga tgaccggtta ccaggagact ctcaccgacc cgtcctaccg ccgccagatc
     1261 gtggtgcaga ccgctccgca tatcggcaac accggctgga atgacgagga cgacgagtcc
     1321 caccggatct gggtggccgg gtacgtcgtg cgcgatccgg cccggatcac ctccaattgg
     1381 cggtctcgcc ggtcgctgga gcaggagctg tccgcccagg gcgtcgtggg gatatccggc
     1441 gtggacactc gggccttgac ccgccggctt cgtgagcacg gctcgatgcg ggccggggtc
     1501 ttctccggtg acgcgctggc ccagccggcg cagctggtgg cgcgggtgct ggccagcccg
     1561 tcgatggtcg gggcggatct ggtcggcgag gtcactgccg cccagcccta cctggtgccg
     1621 gccggtcctg ctcagcaccg gttccgggtg gtggcgctgg acctggggat caagtccaac
     1681 accccgcgga tgctggccgc ccggggcatc gagacgcacg tcctgccggc caccgccacc
     1741 ctcgagcagg tgctggagcg cgaacccgat ggtgtgttcc tgtccaacgg tccgggcgat
     1801 ccggccaccg cggaccacgc ggtttcgttg acccgcgcgg tgctggc
//
LOCUS       DATADN010000021         3824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_605744, whole genome shotgun sequence.
ACCESSION   DATADN010000021 DATADN010000000
VERSION     DATADN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605744"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..175
                     /locus_tag="VHH53_00260"
                     /pseudo
     CDS             35..175
                     /locus_tag="VHH53_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(172..855)
                     /locus_tag="VHH53_00265"
     CDS             complement(172..855)
                     /locus_tag="VHH53_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEX2288652.1"
                     /translation="MSRTRELAAGGRAVEVEPERLAGFLERFAAGHGGAVSTTIGPRR
                     VDLVADDGWTASIPVPFSTLEAQPGTYSGLAVDDLIGHLMVPRTMGLLLVRLGGYSVG
                     IARAGVVLTSSTGSRLVHGRSAAGGQSQQRFARRREGQARVALRAAADTAARVLLPGA
                     AQLDAVVLGGDATALRSLIADPRLTALLGQAESRVFDIPEPRRAVLDDVAQRARCVEV
                     GLHPPVRPD"
     gene            892..2109
                     /locus_tag="VHH53_00270"
     CDS             892..2109
                     /locus_tag="VHH53_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX2288653.1"
                     /translation="MIDVLVAGGGPAGWAVAAACARLGLHTELVDPAPDRPWRATYGS
                     WRAELPDDTEAAVAATGHGEAIGTTGHRLGWEYVVLDNSALRDGFATAPVKVVKGRVR
                     DVDPAPEGVTVDVDGSQARTAVLLDATGAARSILGASLRRPAAEQTAVGVVVDADAAT
                     TVVTPGSALFMDWRPHHGELGWPTFLYAIPVTPDRVLLEETSLARRPGLSLAVLRRRL
                     HARLAHHGIAVPAADEERVRIPVDRPLPTASAWRRPVVPFGAASPLVHPATGYSVAAA
                     LQLAPRLATAVRDALQRGGSAAAGAAAWQTVWSPAALAVHTLRRRSLESLLRFPPELV
                     PQFFDVFFALPQRHRWAYLTGREDVTGSAAAMGALFTASPWWLRKRLVAGALDPRTLA
                     APGDGGPVRNVGR"
     gene            complement(2090..2581)
                     /locus_tag="VHH53_00275"
     CDS             complement(2090..2581)
                     /locus_tag="VHH53_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX2288654.1"
                     /translation="MPTFDAAAVDCRVQVFREGMLTSFGHDVTLQVTRLSLEVGDDDK
                     VVADFDATSLRVTTEAVSASDRKDIEKNAQKTLETQKHPKIEFRSVSVARDGDRARIE
                     GDLTLHGVTNPISVEARDDGKQWSAKLVLDQRKFGIKPFSAMLGALKVKPDVEVHITV
                     PHS"
     gene            complement(2605..3264)
                     /locus_tag="VHH53_00280"
     CDS             complement(2605..3264)
                     /locus_tag="VHH53_00280"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX2288655.1"
                     /translation="MLATARLELGQIRRIRAHFGGTVNDVLLSVLAGALREWLIGRGD
                     LVKGSVLRAFIPVSRRARSSDRIGRNQLSGYLCELPVSEPDPRERLLKIRAAMERNKA
                     AGADRGVGAIPLLADRLPPAVHRLAAPVAGQGAALLFDLMVTSIPLPAMRFTLDGAEL
                     EEVFPLAPLAPGQALVVGLSWYRDSAYVTLLADREGFPDVQRLADAIAPAAAAQDVAS
                     G"
     gene            complement(3280..>3824)
                     /locus_tag="VHH53_00285"
     CDS             complement(3280..>3824)
                     /locus_tag="VHH53_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX2288656.1"
                     /translation="CLLARRAQRQPALRQRVQPALSALGAATWTEDAGFRAADHIHLH
                     HVDGPGRADKVVALAADLMVRPLPRTRPLWEFHVISGVGGGRFAILVKMHHALGDGLS
                     ALDIGLRVLDESRPRNGGTPALPASTPPSSTPSSMLSRLWRLRGAVEDAVEQVAEATG
                     IAASVLRRVRLPPPGSPLVI"
BASE COUNT          563 a         1352 c         1338 g          571 t
ORIGIN      
        1 gggataggcc tgggcgacgt gctcctcgcc gtgggcctat cccatgaaac ggctgggtac
       61 acccgccgac gtcgctgcgc tcgtcgcgtt cctcgtgtcc gacgccgcgt cgtggatcac
      121 cggggagacg gtgcgagtgg atggcggcct actcgccacc gggacgccgc gttaatccgg
      181 ccgcaccggg gggtgtagcc caacctcgac gcagcgggca cgctgggcga cgtcgtcgag
      241 caccgcgcgg cgcggctcgg ggatgtcaaa cacgcgggac tcggcctgcc ccagcagcgc
      301 ggtcagccga ggatcggcga tcaggctgcg cagtgccgtg gcgtccccgc cgagaacgac
      361 ggcatccagc tgcgctgcgc cgggcagcaa tacccgcgcc gcggtgtccg ccgcggcgcg
      421 cagcgccacc cgcgcctggc cctcgcggcg ccgggcgaag cgttgctggg actgcccacc
      481 cgcggcggag cgtccgtgca ccaggcggct gccggtgctg gacgtgagga cgaccccagc
      541 gcgggcgatc ccgacgctgt acccgcccag gcgcaccagc agcagcccca tcgtgcgcgg
      601 caccatgagg tggccgatca gatcgtccac ggcgagcccg gagtacgtgc cgggctgcgc
      661 ctcgagcgtg ctgaaaggca ccgggatcga cgctgtccag ccgtcatcag cgaccaggtc
      721 cacccgccgc ggcccgatcg tggtggacac tgccccgccg tgcccggcgg cgaaccgctc
      781 caggaacccg gccagccgct cgggctccac ctccaccgcg cgcccaccgg cggccagctc
      841 cctggtgcgg ctcactccgg cacgctagcg cgtggtcgta ggctgcacct cgtgatcgat
      901 gtgctggtgg ctgggggtgg cccggcggga tgggcggtgg cggcggcctg cgcgcggtta
      961 gggctgcaca ccgagctggt ggacccggcg ccggaccggc cttggcgcgc cacttacggc
     1021 tcgtggcggg cagagctgcc cgacgacact gaggcagccg tggcggcgac tggccacggt
     1081 gaggcgatcg gcactaccgg gcaccggctg ggctgggagt acgtggtgct ggacaacagc
     1141 gcgctgcggg acgggttcgc caccgcaccg gtcaaggtgg tgaagggccg ggtgcgggat
     1201 gttgacccgg ctccggaggg ggtcaccgtc gatgtggacg gtagccaagc ccggactgct
     1261 gtcctgctcg acgcgaccgg tgcggcccgc tcgatcctcg gcgcctccct ccgccggcca
     1321 gcagccgagc agaccgcagt gggggtggtg gtcgatgccg acgccgctac gactgtggtg
     1381 actccgggca gtgcactgtt catggactgg cgaccgcacc acggggaact tggatggccc
     1441 acgttcctct atgcgatacc ggtgacgccg gatcgcgtgc tcctcgagga gacgtcgctg
     1501 gcccgccgtc cggggctcag cctggcggtg ctgcgtcgcc ggttgcacgc ccggctggcc
     1561 caccacggca tcgccgtacc ggcggccgac gaggagcggg tgcgcatccc ggtggaccgt
     1621 ccactcccta cggcttcggc ctggcgccgg ccagtggtgc cgttcggggc agcaagccca
     1681 ctggtgcacc ctgcgaccgg ctacagcgtg gccgcggcgc tgcagctggc gccacggctg
     1741 gccacggctg tccgggacgc gctgcagcgc ggcggttcag cggctgccgg agccgccgca
     1801 tggcagacgg tgtggagccc ggccgcgctg gcggtgcata ccctgcgacg gcgcagcctc
     1861 gaatcgctgc tgcgcttccc gcccgagctt gtcccgcaat tcttcgatgt gttcttcgca
     1921 cttccgcagc ggcaccgctg ggcttacctc accggccgcg aggacgtcac cggcagcgcc
     1981 gctgccatgg gcgcgttgtt caccgcatcc ccgtggtggc ttcgcaagcg gctggtcgcc
     2041 ggtgcgctcg acccgcgcac gctggcagcc cccggggacg gcggtccggt caggaatgtg
     2101 ggacggtgat gtgcacctcg acgtcaggct tgaccttgag cgcgccgagc atggcagaga
     2161 acggcttgat gccgaacttg cgctgatcca ggacgagctt ggcgctccac tgcttgccgt
     2221 cgtcgcgagc ctcgacagag atcggattcg tcacgccgtg cagcgtcagg tcgccttcga
     2281 tgcgggcccg atcgccgtcg cgggccactg acactgagcg gaactcgatc ttcgggtgct
     2341 tctgggtctc gagtgtcttc tgggcgttct tctcgatgtc cttgcgatcg gatgccgaga
     2401 ccgcctccgt tgtcacgcgc agcgaggtcg cgtcgaagtc ggctacgacc ttgtcgtcgt
     2461 cccccacttc cagggacagc ctcgtgactt gcagggtgac gtcgtgtccg aaagacgtca
     2521 gcattccctc ccgaaacacc tggacacggc agtccacggc agcagcatcg aatgtcggca
     2581 cggcgcaagc ttagcttgat gcgatcaccc actggcaacg tcttgagcag ccgccgcagg
     2641 cgcgatcgcg tcggcgagcc gctgcacgtc cgggaagccc tcccgatcgg cgagcagggt
     2701 gacatatgcg ctgtcccggt accaggacag cccgacaacg agggcctggc cgggcgccag
     2761 cggcgccagc gggaacacct cttccaactc cgcgccgtcc agcgtgaacc gcatcgctgg
     2821 cagcgggatg ctcgtcacca tgaggtcgaa cagcagtgcg gcgccctgac cggctacggg
     2881 ggccgccagg cggtgcaccg caggcggcaa ccggtcagcg agcagcggta tcgcaccaac
     2941 gccgcggtcg gcgccagctg ccttgttgcg ctccatcgcc gcgcggatct tgagcagccg
     3001 ctcgcgcgga tcgggctcgc tgaccggcag ctcgcagagg tagccggaca gctgatttcg
     3061 tccgatgcgg tcgctggacc tggcccgccg gctgaccggg atgaacgctc ggagcaccga
     3121 ccctttgacc aagtcgccgc gaccgatgag ccactcccgt aatgcaccgg ccaacactga
     3181 cagcaggacg tcgttcaccg tgccgccgaa gtgtgcccgg atccggcgta tctgcccgag
     3241 ctcgagccga gccgtcgcta acacacgctg gcccgaggac tagatcacca gcggtgagcc
     3301 cggtgggggt aagcggaccc gacgaagcac cgacgcggcg atgccggtcg cctctgctac
     3361 ctgctctacc gcatcctcga cagcaccgcg cagacgccac aaccgggaca gcatgctcga
     3421 aggcgttgac gatggcggcg tcgaggctgg cagggccggt gtgccgccgt ttcgtggtct
     3481 ggattcatcc aacacccgca ggccgatatc cagtgcactc agcccgtcgc ccaacgcatg
     3541 atgcatcttg accagtatcg cgaaacggcc gccgcccaca cctgatatga cgtggaactc
     3601 ccacagcggg cgggttcgcg gcagcggtcg caccatcaga tcggcggcta atgccactac
     3661 cttgtcggcg cgtccgggac cgtcaacatg atgcaggtga atgtgatccg cggcccggaa
     3721 tccagcatct tcagtccacg tcgccgcgcc cagcgcagac agggccggtt gcaccctctg
     3781 ccgcagggct ggttgacgct gggcccggcg ggccagtagg cagg
//
LOCUS       DATADN010000022         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_756667, whole genome shotgun sequence.
ACCESSION   DATADN010000022 DATADN010000000
VERSION     DATADN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756667"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VHH53_00290"
     CDS             <1..1511
                     /locus_tag="VHH53_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2288657.1"
                     /translation="AFAQQGQVWDSPPGGSPLSRTGVDGVVTVLSPVRTGQLASSCTG
                     SAGKHHERPGATVPEPGRTRGQYHIDRSSEVAMATSHDTDIALFDILDPAFRVDSPEV
                     RAAADAHWWARTSVGIAVLRYRECLALLRDRRLRHGISDLVAAYGITSGAFADWLCMT
                     LTSAEGQAHQRLRRLVSAAFTQRSVDVLRPFMWAKAHELIDGFAGNGSCEFMTAFAEP
                     YPAWVIAELLGIPAERFDAFVGWATDIGLGISPAAATERDRIDAAVTGLYACCDELTA
                     QRRANPGEDLISALIAAEDDGQRLTTDQLRVMVSSLVFAGQDTTRNQLGLAMTTFTHH
                     PQQWRLLAKRPELASTAVEELMRVSPATPVIARVATEDFTFQDVDIPAGTHVALFIAA
                     ANTEPGTFGDAPFDITAQRASHLTFGGGIHYCLGNWLARTEMREALPILASRLPDIEL
                     NGPVASRPHIGITGPITLPLRFTARRDDHSERLDPDIQNHSTSIPTHQPLGS"
BASE COUNT          280 a          583 c          482 g          259 t
ORIGIN      
        1 ctgccttcgc gcagcagggg caggtctggg acagcccgcc cgggggctcg cctttaagtc
       61 gcactggcgt tgatggggtc gttaccgtgc tgtcgcccgt gcgcacgggc cagctggcca
      121 gttcctgcac agggtcggcc gggaagcacc acgaacgccc cggtgccacc gtcccagaac
      181 cgggccgtac ccggggtcag taccacatcg atcgctcaag cgaggtcgcg atggccactt
      241 cccatgacac tgatatcgcc ttgttcgata tcctcgatcc agccttccgg gttgattctc
      301 ccgaagtcag agcggccgcc gacgcgcatt ggtgggcgcg tacctcagtg gggatcgcgg
      361 tgttgcgcta ccgggagtgt ctggcgttgc tgcgggaccg ccgcttgcgc cacggcatca
      421 gtgacctcgt ggccgcctat gggatcacct ccggcgcatt cgccgactgg ctgtgcatga
      481 cgctgaccag cgccgagggc caggcgcacc agcggctgcg tcgcctggtc agtgcggcgt
      541 tcacccagcg cagtgtcgat gtgctgcggc ccttcatgtg ggccaaggcc cacgagctga
      601 tcgacggctt cgccggcaac ggcagctgcg agtttatgac agccttcgct gagccctacc
      661 cggcctgggt gatcgccgaa ctgctcggca tccccgccga acgcttcgac gcgtttgtcg
      721 gctgggccac cgacatcggg ctcgggatca gcccggccgc cgcaacggaa cgggaccgca
      781 tcgacgcagc cgtcacaggc ctttacgcct gctgcgacga actaaccgca caacgccgcg
      841 cgaatccagg cgaagatctg atctccgcgc tgatcgccgc agaagacgac ggccagcgcc
      901 tgaccaccga ccagctgcgc gttatggtca gcagcctggt cttcgccggc caggacacca
      961 cccgcaacca actcggcctg gcgatgacca ccttcaccca ccacccccag cagtggcgac
     1021 tgctcgccaa gcgacccgag ctggccagca ccgccgtcga ggaactgatg cgagtcagcc
     1081 ccgccacgcc agtcatcgcc cgggtggcta ccgaagactt caccttccaa gacgtagaca
     1141 taccagctgg tacccacgtc gcgctgttca ttgccgccgc caacaccgaa cccggcacct
     1201 tcggcgacgc gccattcgac atcaccgcgc agcgcgccag ccacctcacc ttcggcggcg
     1261 gcatccacta ctgcctcggc aactggctgg cccgcaccga gatgcgcgag gcactgccaa
     1321 tcctggcgtc ccgacttccg gacattgagc taaacggacc cgtggcctca cggccccaca
     1381 tcggcatcac cggcccgatc accctgcccc tgcgattcac cgcaaggcgc gatgaccaca
     1441 gcgaacgact ggaccccgac attcagaacc attccacgtc gatccccacc catcagcctc
     1501 tgggctcgta ggtagggtct gtggcattag caggtggcag acatactcga tgccgtagtc
     1561 gccctagtca gatggcgagg gtgagttcgg tgaagatcgc cggg
//
LOCUS       DATADN010000023         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_304543, whole genome shotgun sequence.
ACCESSION   DATADN010000023 DATADN010000000
VERSION     DATADN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304543"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..81)
                     /locus_tag="VHH53_00295"
     tRNA            complement(8..81)
                     /locus_tag="VHH53_00295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Arg,seq:tct)
     gene            275..2293
                     /locus_tag="VHH53_00300"
     CDS             275..2293
                     /locus_tag="VHH53_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX2288658.1"
                     /translation="MTVEASHRHVAAVRARVGVLVAVAALLATAVGTGLVVALGGDGL
                     AQLGLPDPGLLTRAGLPAVRVLAECAGVVTVGSLLLAAFLVPPQASGYLDTGGYAAVR
                     TARIAALVWALMSALMVPLLGAQALGRPVTEVLNPLLLGSLVSELSQPGAWALTAGIA
                     LVLAGFCWIALTWGAAVWLFVLSVVGLMPVALTGHSSAGGAHDVATSSLLYHLVAAAL
                     WVGGLIALLAHLARRGDHAGLATHRFSKLALVCWIVMAASGVINALIRVTPDQMLSAY
                     GLLVVGKVTALLVLGVVGWLHRCSAVAAMVNRGDRSAILRLGGVEVLIMFATIGIAVA
                     LSQTAPPGGIAAQLSRTEVLLGYDLAGPPTLERLLLEWRLDLVFGVAALVLAGAYLAG
                     VRRLQLRGDAWPVGRTVAWLTGCATVLIATSSGIGRYSMAMFSVHMGVHMLLTMVAPI
                     LLVLGGPVTLALRALPPTGKGNPPGAREWLQAFTHSWIIRMATHPLMALSMYIASFYV
                     MYFSGLYDAAAPSHWAHLVMNAHFVVMGYAYYWLIVGIDPAPRRLPYLGKLGLLVAAM
                     PFHAFFGISLMGSTTVIGGDFYRTLALPFVPDLLADQRVAGAMAWSLGEIPMVVVLVA
                     LLVQWARSDAREARRFDRRAESDGEAELVAYNALLAQLAERPPVRDNK"
BASE COUNT          315 a          819 c          893 g          495 t
ORIGIN      
        1 ggtgttgcgc ccccggcagg actcgaacct gcggcctagc gattagaagt cgcttgctct
       61 atccggctga gctacggggg ccccgagcgc gggtaagcgc ccgcgagcag agtaaccagg
      121 tgccgccacg gcggtagctg cccctgctgg accgcgttca gcaggctcag cggggtgacc
      181 aggtgcccca taaccccgtt gagcccgctg aatgggattc gggggcagtt gggtaggagg
      241 cgggcaagac gagtgagcgc ctagtctggc gcgtgtgact gtcgaggcct cccaccggca
      301 cgttgccgcg gtacgggctc gggttggcgt gctggtcgcc gttgctgcct tgctggctac
      361 ggctgtcggc acgggactgg tcgtcgctct gggcggtgac gggctcgccc agctgggatt
      421 gcccgatccg gggttgctca ctcgggccgg gctgccagcc gtacgggtgc tggccgaatg
      481 cgccggggtg gtgacggtcg ggtccttgtt gttggccgcg ttcctggtgc ccccgcaagc
      541 ctccggctac ctcgacaccg ggggttacgc cgctgtccgc acagcgcgga tcgctgccct
      601 cgtctgggca ctcatgtcgg cattgatggt tccgctgctt ggtgcgcagg cgctgggtcg
      661 cccggtgacc gaagtgctga atccgctgct gctgggatca ctggtgtctg agctgtccca
      721 gcccggggcc tgggcgctga ccgcggggat agccctggtg ctggctgggt tctgctggat
      781 tgcgctgacc tggggtgcgg cggtgtggct ctttgtgttg tccgtcgttg ggctgatgcc
      841 ggtggcgctg accgggcact cctcggccgg cggcgcccac gacgtcgcca ccagcagcct
      901 gctctatcac ctggttgccg ccgcactatg ggtcggcggc ctgatcgccc tgctggcgca
      961 cctggcccgt cggggtgacc acgccggact cgctacccac cggttctcca agcttgcgct
     1021 ggtctgctgg atcgtgatgg ccgccagcgg ggtgatcaac gcgctgatcc gggtcacccc
     1081 cgaccagatg ctgtctgcct acgggttgct ggtagtgggc aaggtcaccg cactgctggt
     1141 gctgggcgtg gtcggctggc tgcaccggtg cagcgcggtg gccgcgatgg tcaatcgcgg
     1201 cgatcgctcg gccatcctgc ggctgggtgg cgtcgaggtg ctgatcatgt tcgcgaccat
     1261 agggatcgcg gtggcgctga gccagaccgc accgcctggc ggaatcgcgg cgcagctgag
     1321 ccgcaccgag gtactgctcg gctacgacct ggctggccct cccaccctgg agcggctgct
     1381 cctggagtgg cgtctggatc tggtgttcgg ggtcgccgcg ttggttttgg ccggtgccta
     1441 tctcgccggg gtccggcggt tgcagctgcg tggcgacgct tggccggtcg gccgcaccgt
     1501 ggcctggctc accggctgcg cgaccgttct gatcgccaca tcatcgggca tcggccgata
     1561 ctcgatggcc atgttcagcg tccacatggg tgtgcacatg ctgctgacga tggtggcacc
     1621 gatcctgctc gtgctcggcg gaccggtgac cctggcgctg cgggccctgc ctcctaccgg
     1681 caagggcaac ccgcctggag cgcgggaatg gctgcaggcg ttcacccact cttggatcat
     1741 ccggatggcc acccatccgt tgatggccct gagcatgtat atcgcctcgt tctacgttat
     1801 gtacttcagc ggcctctacg atgccgccgc gccgtcacac tgggcgcatc tggtgatgaa
     1861 cgcacacttc gttgtgatgg gttacgccta ctactggctc atcgtcggca tcgaccccgc
     1921 accccgccgg ttgccatacc tgggcaagct cggcctgttg gtggccgcga tgccatttca
     1981 cgcgttcttc gggatctcac tgatgggctc gaccacggtg atcggcggtg acttctaccg
     2041 cacactagct ctgcccttcg tgccggacct gctggccgat cagcgcgtgg ccggagcgat
     2101 ggcgtggtcg ttgggcgaga tcccgatggt ggtcgtgttg gtggcgctgt tggtgcagtg
     2161 ggctcgctcc gacgcccgcg aagcccgccg attcgaccgg agggccgaat ccgacggcga
     2221 agcagagctg gttgcctaca acgctttgct tgcccagctc gctgagcggc cccctgtccg
     2281 cgacaacaag tgacattgct ggaggcgccg ttaggagccg aagtgcggct tcggtcggct
     2341 agcaccgatc accgacaatc cccagctccg gtcggtggcg gcagccaggt tatgcacatc
     2401 tgggccctcc atccccatgt cgatcaaggc tggctaccgg agccacttct ggccgcgcag
     2461 cctgggcggt accggccgag gggctcggct ccccgccgca ggcggcgtgg aaggtgcaca
     2521 gg
//
LOCUS       DATADN010000024         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1360007, whole genome shotgun sequence.
ACCESSION   DATADN010000024 DATADN010000000
VERSION     DATADN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360007"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VHH53_00305"
     CDS             <1..311
                     /locus_tag="VHH53_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX2288659.1"
                     /translation="PLRLRGDREWPLYPLQRGRNYVNVGFWSTVPIAPGGTGSDANRA
                     IERQVDAVGGHKGLYSDSFYSREDFDARYGGEHYAALKERYDPDRRLADLYAKAVRRR
                     "
     gene            308..>1516
                     /locus_tag="VHH53_00310"
     CDS             308..>1516
                     /locus_tag="VHH53_00310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2288660.1"
                     /translation="MTTFRRSGPTTASTTDRLSLAGIVDLLTDGRFPVRFTAYDGSAT
                     GPPNSEIGVHLGTPRGASYLLTAPGHLGMARAYVAGDLRASGVHPGDPYQAMQLMADE
                     VHFRRPTMAELALIVRSLGWEVLHPVPPPPQEARPRWRRVLEGLAHSRYRDSEAVQHH
                     YDVSNRFYELVLGSSMTYTCSVFDRSDADLEQAQERKHELIADKLGLQAGMRLLDVGC
                     GWGGMVRYAARRGVRALGVTLSAQQASWAQQAIVREGLTKIAEVRHLDYRDSPGRDYD
                     AVSSIGLTEHVGVHSYPSYFGLLLGKLRPGGRLLNHCITHPHNRTPGQTELFIDRYVF
                     PDGELAGAGKIITEVQDAGLEVRHVENLREHYALTLKVWCANLVAHWDECVAEVGEAT
                     ARVWGLYMAGS"
BASE COUNT          246 a          500 c          512 g          258 t
ORIGIN      
        1 gcccgttgcg gctgcgcggg gaccgcgagt ggccgctgta tccgctgcaa cgcggccgca
       61 actacgtcaa tgtgggcttc tggtcgaccg tgccgattgc acccggcggc accggcagtg
      121 acgccaatcg ggctatcgag cgccaggttg acgcggtggg cggccacaag gggctctact
      181 cagactcgtt ctactcacgg gaggacttcg acgctcgata cggcggtgag cattacgcgg
      241 cgctgaagga gcgctacgac ccggaccggc gactggcgga cctctatgcg aaggcggtgc
      301 gacgtcgatg acgaccttcc ggcgctctgg tccgacgacg gcatcgacga cggaccggct
      361 gtcactagcg ggaatcgtgg acctgctcac cgatggccgc ttcccggtcc ggttcaccgc
      421 ctacgacggt agcgccaccg gtccacccaa ctccgagatc ggcgtgcatc tgggcactcc
      481 acgcggtgcc agctacctgt taaccgcgcc gggccacctc ggcatggcgc gggcttacgt
      541 cgccggcgac ctgcgcgctt ccggggtgca cccaggcgac ccgtaccagg cgatgcagct
      601 gatggcagac gaggtgcatt tccggcgccc gaccatggca gagctggcgt tgatcgtccg
      661 ctcgctgggc tgggaggtgc tgcatccggt gccgccgcca cctcaggagg cccggccccg
      721 gtggcggcga gtccttgaag gactggccca ttcccgatac cgcgattccg aagcagtgca
      781 gcaccactac gacgtctcca accgcttcta tgagctggtg ctcggctcgt cgatgacgta
      841 cacctgctct gttttcgatc gctcagacgc ggatctggag caagcgcaag agcgtaagca
      901 cgaactgatt gccgacaaac tgggcttgca ggccgggatg cggctgctcg acgtcggttg
      961 tggctggggt ggcatggtgc gctacgccgc ccgacgcggg gtccgggcgc tgggcgtcac
     1021 gctgtctgcg cagcaggcca gttgggccca acaggcgatc gtccgggagg gcctgaccaa
     1081 gatcgccgaa gtgcgtcact tggactaccg ggactcccct ggccgcgact acgacgcggt
     1141 cagctcaatc ggcctcaccg agcacgtcgg agtgcactcc tacccgtcct atttcgggct
     1201 gctgctgggc aagctgcgcc ccgggggccg gctgctcaac cactgcatca cccatccgca
     1261 caaccggacg ccaggtcaga ccgagctgtt cattgaccgc tacgtgttcc ccgacgggga
     1321 gcttgccggt gctggcaaga tcatcaccga ggtacaggac gccggtttgg aggtacgcca
     1381 tgtggagaat ctgcgggagc actacgcgct gacgctcaaa gtgtggtgcg ccaacctcgt
     1441 cgcgcactgg gacgagtgcg tggccgaggt cggcgaagcc accgctcgag tgtggggtct
     1501 gtacatggcc ggttct
//
LOCUS       DATADN010000025         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_756992, whole genome shotgun sequence.
ACCESSION   DATADN010000025 DATADN010000000
VERSION     DATADN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756992"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..869)
                     /locus_tag="VHH53_00315"
     CDS             complement(51..869)
                     /locus_tag="VHH53_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288661.1"
                     /translation="MTYTHVTVHDMTSVMPRGGLEAVLQRAVESGILSADQAQAVLVA
                     ERIHGEQFGGGRRLPVTEALGYLAGLLALSGAVTLAIQYWREVPIAGRIGLLAAVAAT
                     TWLVGARIGDGSAPALIRLRGALWFASSAAVAALAGQIAWDVVHAGEATVWLAAGAAA
                     AVHAGLLWQLRDRPAQHLACLIGVLAAAGGAAGLTDLDSAIGLAIAAVGVAWVVAGWS
                     ATLRPPTMALVGGGIAVLTGAGVTGVGWSDAAPLLGLAAVTALVAVGVGTDRTR"
     gene            1472..>1731
                     /locus_tag="VHH53_00320"
     CDS             1472..>1731
                     /locus_tag="VHH53_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX2288662.1"
                     /translation="MFVDRVDAGRQLGRRLQYLRGEDVVVLGLPRGGVPVAAEVALAL
                     GAPLDVIMVRKLGVPFQPELAMGAIGEGGVRILVPETVRRAGV"
BASE COUNT          279 a          598 c          594 g          260 t
ORIGIN      
        1 gccgatggtc cagggcaggt agacgaacgc gccggtgagg ccgacgatgg tcagcgcgtc
       61 cggtcggtcc ctacccccac tgctacgagc gcggtcacag cggccaaccc cagcagcggg
      121 gcagcgtcgg accagccgac cccggtgaca cctgcgccgg tcagcaccgc gataccgcca
      181 ccgaccagag ccatcgtggg cggacgaagc gttgccgacc agcctgcgac cacccatgcg
      241 acgcccaccg cggcgatcgc cagtccgatc gcactgtcca ggtcggtcaa acccgccgca
      301 ccacccgctg ccgcgagcac accaatcaag caggcgagat gctgagcagg ccgatcccgc
      361 agttgccaca gcagccccgc gtgcaccgcc gccgccgcac ccgctgccag ccacacggtg
      421 gcctccccgg cgtgcacgac atcccacgct atttggccag caagtgccgc caccgccgcc
      481 gaggacgcga accagagcgc gccgcgcagc cggatcagcg cgggcgccga accgtcaccg
      541 atgcgggcac ccaccagcca ggtcgtggcg gcgacagcgg ccagcagccc gatccgaccg
      601 gctatgggca cttcccgcca gtactggatg gccaaagtga ctgctccgga cagcgccagc
      661 agcccggcga ggtaacccag cgcctccgtc accggtagcc ggcggccacc accgaactgc
      721 tcgccgtgga tccgctccgc gacgagcacg gcttgcgcct ggtcggcgct gagtatcccg
      781 gactcgacgg cgcgctgcag cacagcttcc agaccaccgc gaggcatcac cgacgtcatg
      841 tcgtgaaccg tcacatgggt gtatgtcatg gttcaaggtc ggctgcggac tgtcacctgg
      901 aggcttgtcg cggtgacacg gcatactgcg gcggtatgcg cgtccggatc ggtttcgcag
      961 cgttcagcat cgtgggcttg gggctactca gtgcggcttg ctctgggtca gctggcccgg
     1021 cttcagctgg ctctggggca acacgctcgg agtcgcctag ccagggggag gccagaccga
     1081 tggccgaacc agctgaggct gcgccgtcgg tgagctggtt gcgggcgacc cccgggcagg
     1141 gcacgccgaa ctccgcagtg tcgctcgatg tggcttgcct ggatgatctt ggcgccgtgc
     1201 agtcgccggt tctcgatatc ggtccgatga agggtaaccc cgaaggccac caaccgtggc
     1261 gcctgttggg caccgccacc gtccggccgg acgcagttgc aggtcgatac cgaattgtca
     1321 cgacatgcgg cgccagcgag ctctcaaccg acttcaccgt cgtaccaggc ccgtctcggt
     1381 agggatccgc gagtttcacc cgcccagacc gggataatcg gttcgttgga ctgatcgtcg
     1441 acggaccgag cgggcaagga ggtggcgcgg ggtgttcgtc gatcgggtcg acgctgggcg
     1501 gcagctaggg cggcggctgc agtacctgcg cggcgaggat gtggtagttc tgggacttcc
     1561 gcgcggtggg gtccccgttg cggcagaggt cgcgttggct ctcggcgctc cactggacgt
     1621 gatcatggtt cggaagctcg gcgtgccgtt tcagcccgag ctggcaatgg gcgccatcgg
     1681 tgaaggtggc gtgcggatcc tcgtgccgga gacggtacgt cgcgcgggtg t
//
LOCUS       DATADN010000026         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_154078, whole genome shotgun sequence.
ACCESSION   DATADN010000026 DATADN010000000
VERSION     DATADN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154078"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..>1593)
                     /gene="polA"
                     /locus_tag="VHH53_00325"
     CDS             complement(123..>1593)
                     /gene="polA"
                     /locus_tag="VHH53_00325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX2288663.1"
                     /translation="GLTSDTALAAYLVRPGQRSFDLADLVLRYLRRELRAESGDSVQP
                     SLFEGEDAAARVADEILRARAVAELAVALDAELDAIAGRSLLTDLELPLLAVLAELEA
                     AGIAVDGDVLAGLEAGFSAAVKQAEQDCFGVIGREINLGSPKQLQAVLFDELGMPKTK
                     RTKTGYTTDADALQTLYVATAHPLLAHLLAHRDATRLKTTVDGLLKTVADDGRIHTTY
                     NQTIAATGRLSSTEPNLQNIPIRTQSGRQIRRAFVVGPGYSELMTADYSQIEMRIMAH
                     LSEDTALIESFTSGYDFHAATAARVFAVDPAAVTGEQRAKIKAMNYGLAYGLSAFGLS
                     AQLRISTEEARELMEEYFARFGGVRDYLRTVVEKARRDGYTATILGRRRYLPDLISDN
                     RQRREMAERMALNAPIQGSAADIIKVAMLNVARALRAEALRSRMLLQVHDELVLEIAD
                     GEHAAVETLVRREMGSAYALDVPLEVSVGFGRCWDDAAH"
BASE COUNT          269 a          542 c          509 g          273 t
ORIGIN      
        1 cgcttgtcga agcccagctt gtcgacgaag aactcgacca tcgtgtcctg atccgcgacg
       61 gccagcatcg cccagtggat tcgagtgatc atggtgcacc ctctgtctcc tgcaagcagg
      121 tctcagtgcg ccgcatcgtc ccagcatcgt ccgaatccga ccgagacctc cagcgggacg
      181 tccagcgcgt acgccgagcc catttcgcga cggacgagcg tctccaccgc ggcatgctcg
      241 ccgtcggcga tctccagcac cagctcatcg tgcacctgca gcagcatccg ggaacggagt
      301 gcctcggcac gcagtgcgcg ggcgacgttg agcatcgcga ccttgatgat gtccgcggcg
      361 ctgccctgga tgggcgcgtt gagtgccatc cgctcggcca tctcgcgacg ctggcggttg
      421 tcgctgatca ggtcgggaag gtaccgccgg cgacccagaa tggtggcggt gtaaccgtcc
      481 cggcgggcct tttcgaccac cgtgcgcagg tagtcccgga cgccaccgaa tcgggcgaag
      541 tactcctcca tcaactcgcg ggcctcctcg gtggagatcc gcagctgcgc ggacaggccg
      601 aatgcggaca acccgtaggc caggccgtag ttcatcgcct tgatcttggc gcgctgctcg
      661 ccagtgaccg cggcagggtc caccgcgaac acccgcgcgg cggtggcagc gtggaagtca
      721 taccccgaag tgaacgactc gatcagcgcg gtgtcctcag acagatgcgc catgatccgc
      781 atctcgatct ggctgtagtc ggccgtcatc aactctgagt aaccgggacc gaccacgaac
      841 gctcgccgga tctggcgtcc tgactgggtg cggatcggga tgttctgcaa gttcggttcg
      901 gtggaggaca gccgcccggt cgcggcgatg gtctggttgt aggtggtgtg gatccgccca
      961 tcgtcggcca ccgtcttgag caggccatcc accgtggtct tgagccgggt cgcgtcccga
     1021 tgcgccaaga ggtgggcgag caacggatgc gcagtcgcca cataaagggt ctgcagcgca
     1081 tcggcgtcgg tggtgtaacc ggtcttggtg cgcttggtct tgggcatgcc caactcgtcg
     1141 aacagcacgg cctgcagttg cttgggcgac cccaggttga tttccctgcc gatcactccg
     1201 aaacagtcct gctcggcctg cttcactgct gccgaaaagc cagcctccag ccctgcgagc
     1261 acgtcaccgt cgacggcgat accggcggcc tccagctcgg ccagtaccgc gagcagtggc
     1321 agctccaggt cggtgagcaa ggaccgaccc gctatcgcgt ccagttcggc gtccagtgcg
     1381 acggccagct cggccacggc ccgcgcccgc aggatctcgt cggccacacg ggcggctgcg
     1441 tcctcgccct cgaacaagct cggttgaacg ctatccccgg attccgcgcg caactcccta
     1501 cgcagatagc gcagcaccag gtcggcaagg tcgaaagacc gctggccggg ccgaaccaga
     1561 taagcggcca gcgcagtgtc gctggtcaac ccc
//
LOCUS       DATADN010000027         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_304924, whole genome shotgun sequence.
ACCESSION   DATADN010000027 DATADN010000000
VERSION     DATADN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304924"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VHH53_00330"
     CDS             <1..292
                     /locus_tag="VHH53_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX2288664.1"
                     /translation="ELEEVVISSAGAAANGHPVLAVDDAGRPTAWHHLPGYAETPPLV
                     TVPLSGTVYDAVDAMLDARAPLVVVVDDDEQPRGALRWGSLVNDLRTRGRRP"
     gene            289..1065
                     /locus_tag="VHH53_00335"
     CDS             289..1065
                     /locus_tag="VHH53_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2288665.1"
                     /translation="MTAGPPATTSAADRSPIWIWRNRDLITTPVITVVGLALVLLYTA
                     NRQLDSVEQRALDPSYLWAKFLEHIWLTVASTVVVIAIALPLGIALSRPAVGRIRTGT
                     LAFGGFMQALPAYGVIILLAFILGFGTTTAIIALVLASFLPVLTNTVVGLRQVDPALI
                     EAARGMGMSARRTLLRVEIPLAVPVMVAGIRVALVLNVGTAALTTFIGAGGLGEPISA
                     MLNLGRAQAVFAASAIVAALALLIDWLAGVAERLVSGRVG"
     gene            1141..1914
                     /locus_tag="VHH53_00340"
     CDS             1141..1914
                     /locus_tag="VHH53_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2288666.1"
                     /translation="MVTVLVAGCGGLRSSGPPAQGGSLAQQVSLQGQSYTVGSKTFDE
                     ALVLCQIAIAALESVNAEVSDQCNLGGSDATRNALLGGDIDLYWEYTGTAWVSFLGQQ
                     PIQDSKVQYEAVKDLDLAQNKIVWLQPTPFNNIYSFALKRERAEQLGLRTLSDMAAYI
                     PSDQPGNMCIESVYLNRDDGFPGLQRTYGFQVPSDRLQVLQTGAIYQATTIRRSACSG
                     RCSSPTAVSTAWLTVLQDDKLYYPLYNAAPHHAQRGLRP"
     gene            complement(2329..>2736)
                     /locus_tag="VHH53_00345"
     CDS             complement(2329..>2736)
                     /locus_tag="VHH53_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288667.1"
                     /translation="FDCVEVKPVEVIRQPGRLEVVAGQLRATMTVGRRSWLGILLRAV
                     PRPVATAPAWASLVDPMVRAMLPGVRTRGSATGGRKEWYGAWDHHRLGSVVASWRGTD
                     LGAMTDVFPPVRFGFGSAPRQPCVVRLTTTVQL"
BASE COUNT          478 a          893 c          890 g          475 t
ORIGIN      
        1 ggaactggag gaggtggtga tctccagcgc cggcgctgca gccaacggcc acccggttct
       61 cgcggtggac gatgcgggcc gccccaccgc gtggcaccat ctgcccggtt acgcggaaac
      121 gccgccgctg gtgacggtgc cactgtctgg caccgtctac gacgccgtcg acgccatgct
      181 cgacgcccgc gcgccgctgg tcgtggtggt cgacgatgac gagcagccac gtggcgcgct
      241 gcgctggggc tccctagtca acgacctgcg caccaggggc aggcggccat gaccgctggc
      301 ccgccggcga ccacctcggc agccgaccgc tcccccatct ggatctggcg caaccgtgat
      361 ctgatcacca ccccggtgat cacggtcgtc gggttggcgc tggtgttgct ctacaccgcc
      421 aaccggcagc tggacagcgt cgagcagcgc gcgctggatc cgtcatacct gtgggcgaag
      481 ttcctcgagc acatctggct cacggtggcg tccaccgtgg tggtgatcgc catcgcgtta
      541 ccgctgggga tagcgctgag ccggccggcg gtgggccgga tccgtaccgg gacgcttgcg
      601 ttcggcggct tcatgcaggc cctgccggcc tacggtgtga tcatcttgct ggccttcatc
      661 ctcggcttcg gcaccaccac agcgatcatc gccttggtgc tggcgtcatt cctgccggtg
      721 ctcaccaaca ccgtggtcgg gttgcggcag gtggacccgg cgctgatcga ggccgcccgc
      781 ggcatgggga tgtcggcgcg gcggaccctg ctgcgggtgg agatcccgct cgcggtaccg
      841 gtgatggtgg cagggatccg ggtggcgctc gtgctcaacg tcggcaccgc cgcgctcacc
      901 accttcatcg gagcgggcgg cctcggtgag ccgatcagcg caatgctcaa cctcggccgg
      961 gcgcaggcgg tattcgctgc ctcggccatc gtggcggcgt tggcattgct catcgactgg
     1021 ttggccggcg tcgccgagcg gctcgtgagt ggccgggtcg ggtgacagga gggacgcgtg
     1081 gaatcggcat catcgcgtgt cggccgcggg cgcatcgctg cctggctggc taccttggcg
     1141 gtggtcaccg tgctggtagc cgggtgcggt ggcttgcgca gcagtggccc gccggcacag
     1201 ggcggcagcc tcgcacagca ggtcagcctg cagggccagt cctacaccgt gggcagtaag
     1261 accttcgacg aggcgttggt gctgtgccag atcgcgatcg ctgccctaga gtcggtcaac
     1321 gccgaggtca gcgaccaatg caatctcggt ggcagcgacg caacccgcaa tgcgcttctc
     1381 ggtggcgaca tcgacttgta ttgggaatac accggcaccg cgtgggtatc attcctagga
     1441 cagcaaccca tccaggacag caaggtgcag tacgaggcgg tcaaggattt ggacctggcg
     1501 cagaacaaga tcgtctggct ccagccgacg ccgttcaaca acatctactc cttcgctctc
     1561 aagcgggagc gcgccgagca gctcggattg cgaacgctgt cggatatggc cgcatacatc
     1621 ccctctgacc aacctggcaa catgtgcatc gaaagcgtat atctgaatcg ggatgatgga
     1681 ttcccgggtc tgcagcgaac ttacggcttt caggtcccgt cggatcggct gcaggtactt
     1741 cagaccggtg ccatctatca ggccaccacg atcagaagga gtgcctgttc ggggaggtgt
     1801 tcatcaccga cggccgtatc cacagcttgg ctgacggtgt tgcaggacga caagctgtac
     1861 tacccgcttt acaacgcggc gccccatcat gcacaaagag gcctacgacc gtaacccgga
     1921 cattgccaag gtgctcgcac cgatctccgc agtgttcatc aacgatgggg tggccgagct
     1981 gaaccggtag cgatctgcgg aaggtagatc ggcccgccac gtcgtccgcg attagctttc
     2041 tcagccgagc ttcatcgcaa ggaaccgtga cttctatcat cgaataacaa ggtttaacag
     2101 caccgtcagc atgacggagg ccaataccga gaagagaatc ataaaaaggc agccgaccgc
     2161 cccaccgagg ggacggatcc tcatgcgcgt cgcctccatc gctcagcgaa tcccggacgc
     2221 gtctgtgtgc gcgccgtcgc actgaattac ttaaaggatg taccccggtg ccgggcactc
     2281 aaacccgtgg atgagggtcg tgaatagccc tgaaccgtgc atggctgctc atagctgcac
     2341 ggtcgtggtc aggcgcacaa cgcagggctg gcgtggcgcc gagccgaacc cgaatcggac
     2401 gggtggaaag acgtcggtca tggcaccgag gtccgtcccc cgccagctgg cgactacgga
     2461 tcccagtcga tgatggtccc aagccccgta ccactccttt cgcccgccag tagcgctacc
     2521 tcgggtgcgc acaccgggga gcatggcccg caccattggg tccacgagac tcgcccaagc
     2581 gggggcggtg gccacaggac gcggtacagc tcgcagcagg atcccgagcc agctcctgcg
     2641 cccgaccgtc atggtggctc gcagctggcc ggctacgact tccagccgcc ccggttgacg
     2701 gatcacttca accggtttga cctcaacgca atcgaa
//
LOCUS       DATADN010000028         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_455758, whole genome shotgun sequence.
ACCESSION   DATADN010000028 DATADN010000000
VERSION     DATADN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455758"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VHH53_00350"
     CDS             complement(<1..301)
                     /locus_tag="VHH53_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2288668.1"
                     /translation="MRRETTGYRIFRWTTLTVLTIFVCAPLAVMASTAVKPLNQVQSD
                     FSWIPTEPTLAPFVDMWSTVPLAQYFANSILVAGAATVCSVTVAVLAAYALSRWRF"
     gene            complement(291..1265)
                     /locus_tag="VHH53_00355"
     CDS             complement(291..1265)
                     /locus_tag="VHH53_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2288669.1"
                     /translation="MSIKVDHTIGSRPAGRRAIPPQQRRRPRTWSPYLFLLPALVLEL
                     AVHFGPMVAGLAMSFLKLTQYQLARWWQAPFAGLENFRVVLDIDQPVGASLLSSFLVT
                     LAFTVIVIAVSWIMGIAAAVSLQNARTGRGVLRTLFLVPYALPAFAAVITWRFLFQRD
                     NGMLNHILVDQLGLLEENAFFLIGPNAFWAQCVVAIWRLWPFAFLMLMAGLQSIPAEL
                     YEAGTLDGAGPWRQLREITLPLLSPVNQVLVLVMFLWTFKEFETPFALFGGSAPEEAR
                     LINVEIYQNSFVTWNFGLGAAMSVLLLLFLLLVTGAYLLATRRKLHEA"
     gene            complement(1262..>1569)
                     /locus_tag="VHH53_00360"
     CDS             complement(1262..>1569)
                     /locus_tag="VHH53_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2288670.1"
                     /translation="LVQFLTSVEQQTVINTAYGTIPPIKDAPDGAFQSDEAKVARETI
                     ASRAIPLPRVPQEAQFETLVGNAVVTWLADTATGRQPTADQIRSELEALSARVASGG"
BASE COUNT          289 a          506 c          546 g          228 t
ORIGIN      
        1 ggaagcgcca gcggctgagc gcataggcgg ccagcaccgc gaccgtcaca gaacagacgg
       61 tggccgcacc ggcgacgagg atcgagttgg cgaagtactg cgcgagcggg acggtggacc
      121 acatgtcgac gaagggcgcc agcgtcggtt cggtgggaat ccagctgaag tcgctctgca
      181 cctggttcag cggtttgacg gcggtgctcg ccatgacggc cagcggggcg cagacgaaga
      241 tcgtcagcac tgtcaaggtc gtccaccgga agatccggta gcctgtggtc tcacgcctca
      301 tggagcttcc tcctcgtcgc cagcaggtag gcgccggtca ccagcagcag gaacagcagc
      361 agcaggacgc tcatggcggc gccgaggccg aagttccacg tcacgaacga gttctggtag
      421 atctcgacgt tgatcagtcg cgcttcctcg ggcgcgctgc cgccgaacag cgcgaacggc
      481 gtctcgaact ccttgaaggt ccacaggaac atcaccagga ccaggacctg gttcaccggt
      541 gacagcaacg ggagggtgat ctcgcgcagc tggcgccacg gccccgcgcc gtcgagcgtc
      601 ccggcttcgt agagctccgc cgggatggac tgcaggccgg ccatcagcat gaggaaggcg
      661 aacggccaca ggcgccagat cgccacaaca cactgggccc agaacgcatt cgggccgatg
      721 aggaagaacg cgttctcctc cagcaggccc agctggtcga ccaggatgtg gttgagcatt
      781 ccgttgtcgc gctggaacag gaaccgccag gtgatgacgg cggcgaaggc gggcaacgcg
      841 tagggcacca ggaacagggt gcgcagcact ccccggcccg tcctggcgtt ctgcagcgac
      901 accgccgcgg cgatccccat gatccaggag accgcgatga cgatcacggt gaacgccagg
      961 gtgaccagga aggagctgag cagcgacgct cccaccggct ggtcgatgtc gagcacgaca
     1021 cggaagttct ccaggcccgc gaacggcgcc tgccaccacc gcgcgagctg gtactgggtc
     1081 agcttcagga agctcattgc caggcccgca accatcggtc cgaaatggac cgccagctcc
     1141 agcaccagcg cgggcagcag gaagaggtac gggctccagg tgcgcgggcg ccgacgttgt
     1201 tgcggaggga tcgctcgtcg accggcggga cgggaaccga tggtgtgatc gaccttgatg
     1261 ctcatccgcc cgacgccacc ctcgccgaca gcgcctcgag ctcggacctg atctggtccg
     1321 cggtcggctg ccggccggtg gccgtgtcgg ctagccacgt cacgacggca ttacccacga
     1381 gggtctcgaa ctgcgcctcc tgcggtacgc ggggcagcgg gatcgcgcgg gaggcgatgg
     1441 tctcgcgtgc caccttcgcc tcgtcgctct ggaacgcgcc gtccggcgca tccttgatcg
     1501 gcgggatggt gccgtaggcg gtgttgatga cggtctgctg ctcgacgctg gtgaggaact
     1561 ggacgagct
//
LOCUS       DATADN010000029         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_305245, whole genome shotgun sequence.
ACCESSION   DATADN010000029 DATADN010000000
VERSION     DATADN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305245"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VHH53_00365"
     CDS             <1..535
                     /locus_tag="VHH53_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX2288671.1"
                     /translation="AAGVGGRLLRKPRRRPPMTPFVPRSRSWVSMGMRDVPVDALQAA
                     LAAEHAAVWVYGLAGAFLPDRLSGQLREAAGAHQARRDATERMLIDARAQPVPAEPGY
                     LTPEPVTDAASALRLVITAETDAAAAWRSVVERSPADPVLRTAALDALTQAAVRATRW
                     RAVAGITPLTVPFPGAP"
     gene            556..966
                     /locus_tag="VHH53_00370"
     CDS             556..966
                     /locus_tag="VHH53_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX2288672.1"
                     /translation="MHTEELQIQTGSREQVRDLTTECRAFLHQVGARDGLLHVWVPHA
                     TAGLAVIETGAGSDTDLLAALRELLPADGRWRHRHGSPGHGRDHLLPALLPPYASVPV
                     LGGQLALGTWQSVCLVDTNIDNPVRTIRLSFLPG"
     gene            complement(967..>2688)
                     /locus_tag="VHH53_00375"
     CDS             complement(967..>2688)
                     /locus_tag="VHH53_00375"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2288673.1"
                     /translation="RTLREDPADAEVPSHKLLVRAGYVRRVAPGGYSWLPLGLRVLRK
                     IEQVVREEMDAFGAQEIQFPALLPKEPYEATNRWTEYGPNIFRLKDRKGADYLLGPTH
                     EELFTLTVKGEFSSYKDYPVILYQVQTKYRDEERPRAGILRGREFLMKDSYSFDLSEE
                     GLALSYRLHREAYIKIFDRLGLRYVIVSAMSGAMGGSASEEFLAEADTGEDTFVRSTE
                     SRYAANVEAVTIPAPPELPIDGRPPAQILHTPNTPTIDTLVDHLNGAGLGRTFSAVDT
                     LKNVMVKTRPPGAQECWALLGIGVPGDREVDIKRLAAAVYPAEIALLDDADFAAHPFL
                     VKGYIGPGALAANGVRYLVDPRVVPGTAWATGADRLDHHVVDLLCGRDFIPDGTIEAA
                     QVRPGDPSPDGAGTLVAARGIEIGHIFQLGRKFADAFGLDALGPDGKPIPITMGSYGI
                     GLSRLVGAIAEQSHDPHGLIWPRNVAPADVHVVIAGKDGTVRAASEWLAAELDASGER
                     VILDDRPATSGVKFADAELIGVPTILVVGRGLSSGVVEVKDRATGERTEVPVDAAVAH
                     LQELCGK"
BASE COUNT          402 a          941 c          914 g          431 t
ORIGIN      
        1 cgctgctggc gtcggtggcc gcctgctgcg caagccacgc cgccgtcctc ccatgacgcc
       61 cttcgttccc cgctctcgat catgggtttc gatggggatg agggacgtgc cggtggatgc
      121 gctgcaggct gcgttggcgg ccgagcacgc ggcggtgtgg gtttacgggc tcgccggggc
      181 gttcctgccg gataggttgt ccggtcagct ccgcgaagcc gccggcgcgc accaggctcg
      241 gcgcgatgcc accgagcgga tgctgatcga cgcacgcgcg cagccggtgc ccgccgagcc
      301 gggttacctc actccagaac cggtcaccga cgccgcatca gcgctgcggc tggtgatcac
      361 cgcggagacc gacgcggccg ctgcctggcg ctcggtagtc gagcgcagcc ctgccgatcc
      421 ggtcctgcgc accgccgcgc tggacgcact gacccaggcc gccgtccggg cgacccgctg
      481 gcgcgccgtc gcgggcatca ccccgctgac ggtgccgttt ccaggcgcac cgtaaaccac
      541 cgtaccgtcg tcggcgtgca caccgaggaa ctccagatcc agaccggctc acgcgagcag
      601 gtgcgcgacc tcaccacgga atgccgagcg ttcctccacc aggtgggcgc ccgcgacggc
      661 ctgctgcatg tgtgggtgcc gcatgccacc gccggtctcg cggtgatcga gaccggggcg
      721 ggcagcgaca ccgacctgct cgccgcgctg cgtgagctgc tgcccgccga cggacggtgg
      781 cgccatcggc atggctcccc agggcatggc cgggatcact tactccccgc gttgctgccg
      841 ccctacgcca gcgtcccggt gctcggtggg cagctcgcac tgggcacctg gcagtcggtc
      901 tgcctggtgg acaccaacat cgacaacccg gtacgcacga tccggttgag cttcttgccc
      961 ggctgactat ttcccacaca gctcctgcag gtgagccacc gcggcgtcca ccggtacctc
     1021 ggtgcgttct ccggtggccc gatccttgac ctccacgaca cccgatgaca ggccgcggcc
     1081 gaccacgagg atggtcggca caccgatcaa ctcggcgtcg gcaaacttca ccccagaagt
     1141 ggccgggcga tcatcgagga tcactcgctc gccggacgca tccaactcgg cggcaagcca
     1201 ctcgctggcg gccctgacgg tgccatcctt acccgcgatc acgacgtgga cgtcggctgg
     1261 ggcgacattt cgtggccaga tcaggccgtg cgggtcgtgg ctctgctcgg cgatcgcacc
     1321 gaccagccgg gagagtccga tgccgtagga ccccatggtg atcgggatcg gcttgccgtc
     1381 gggccccagc gcgtccaagc cgaaggcatc cgcgaacttg cggccgagct ggaagatgtg
     1441 cccgatctcg atgcccctgg cggcaacgag ggtgcccgcg ccgtcgggtg acggatcgcc
     1501 ggggcgaacc tgcgccgcct caatcgtgcc gtcggggatg aagtcccggc cgcagagcag
     1561 atcgacgacg tggtggtcga gccggtccgc gccggtcgcc caggccgtgc ccggaaccac
     1621 tcgcggatcg acgaggtacc gcacgccgtt cgccgccagc gcccccggcc cgatgtaacc
     1681 cttgaccaga aacgggtgcg cggcgaagtc agcgtcgtcc aacagtgcga tttcggccgg
     1741 atacaccgcg gccgccaggc gcttgatgtc aacctcgcga tcgccgggaa ctccgatacc
     1801 gagcagagcc cagcactcct gcgcgcctgg tggccgggtc ttgaccatga cgttcttcag
     1861 ggtgtccacg gcgctgaagg tgcggcccaa gcctgccccg ttgaggtggt cgaccagcgt
     1921 gtcgatggtc ggggtgttgg gagtatgcag gatctgggcc ggcggccggc cgtcgatagg
     1981 cagctccggg ggtgccggga tggtgaccgc ttcgacattg gctgcgtacc tggattcggt
     2041 gctgcgcacg aaggtgtcct cgccggtgtc ggcttcggcg aggaactcct cgctggccga
     2101 gccgcccatc gcgccggaca tcgccgacac gatcacgtac cgcagcccga gccggtcgaa
     2161 gatcttgatg tatgcctcgc ggtgaagccg gtaagaaagc gccaatcctt cctcggagag
     2221 gtcgaaggag tacgagtcct tcatcaagaa ctcccgaccg cgcaagatcc cggcgcgggg
     2281 gcgctcctcg tcgcggtact tggtctgcac ctgatagagg atcaccggat agtccttgta
     2341 tgaactgaac tcacccttga cggtgagcgt gaacagctcc tcatgggtgg gtccgagcag
     2401 gtagtcggcg cccttgcggt ccttgagccg gaagatgttg ggtccgtact cggtccaccg
     2461 gttggtggcc tcgtagggct ccttgggcag cagggcgggg aactggatct cctgcgcccc
     2521 gaacgcgtcc atctcctcgc gcacgacctg ctcgatcttc cgcagcaccc gcagccccag
     2581 cggcagccac gagtaaccgc ccggcgcgac gcggcgcacg tagccggcgc ggaccagcag
     2641 cttgtggctg ggtacttcgg cgtcggccgg gtcctcgcgc agggtgcg
//
LOCUS       DATADN010000030         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_757486, whole genome shotgun sequence.
ACCESSION   DATADN010000030 DATADN010000000
VERSION     DATADN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757486"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..678)
                     /locus_tag="VHH53_00380"
                     /pseudo
     CDS             complement(22..678)
                     /locus_tag="VHH53_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(829..2259)
                     /gene="ltrA"
                     /locus_tag="VHH53_00385"
     CDS             complement(829..2259)
                     /gene="ltrA"
                     /locus_tag="VHH53_00385"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX2288674.1"
                     /translation="MNTSDLPLGSFRAERRVLEIQTKLHRWANDDPHCRFDDLFNLVT
                     DPSFLLMAWERVAGNKGARSAGVDGRTVTVVRERIGVEIFLAELREQLKSGSFQPLPV
                     RERMIPKPGSRKRRRLGIPTVADRVVQASLKLVLEPIFEADFVPCSYGFRPNRRAHDA
                     MAETRFLASKTYEWVLEGDITACFDEISHPALMDRVRARIGDKRVLALVKAFLKAGIL
                     TERGTAQDTITGTPQGGILSPLLANVALSVLDEHFAKAPGGPQSTTKQRWTRRQRGLP
                     NYRLIRYADDFVILVSGTRAHAEALLPEVAAVVSTVGLRLSEDKTLITHIDEGLDFLG
                     WRIQRHQKRGTNRHYVYNYPAKKALRSIKAKCKAICRLNVGLPLAVLVHKLNLVLRGW
                     TTYFRPGVSARAFQYLRMVIWRQVFGWLRRKHPNTSWKDLRRRYCDGTWWPRAGGIVL
                     FNPGSVVTTRYRPRGTTIPSPWPSVN"
BASE COUNT          572 a         1176 c          978 g          570 t
ORIGIN      
        1 cgagcgggcg cacgactatc tggagcgctc gatgagtccc ttcgcttccg ggtcagcggg
       61 ccggcagatg atcacctttg tcccgagcgt cccacggaac ccttggcatt gcgcggtgag
      121 ttcgctgcgc ccaccgcgcc accgcccgat ggcgccctca ccatcccaga ctaatacccg
      181 tggcaccgca cccaacccgg cgatgtggcc ccaccaacca gcgaacagat cctcggcggc
      241 ccgcgtcggg atcagcaccc ccgacaacca ccgcgaataa cccgtgacca tggtcaacac
      301 cggcagcgcc ttggcggtgc gaacctgccc gcagcccacc ggtagctcga tgtcaggaaa
      361 ccagaagtcg cactgcgcga tctcccccgc cgcatagcag gtccgcgacg ccggatccgg
      421 cggcaggtag accggccgca gctcccgaac ccggtcggtg aacaccgtca tcccccgcga
      481 ccaaccgacc cgctcggcga tcaccgtcgc cggcatcgtc ggacacgcca ccagcaactc
      541 acgaatccgc ggctcgacct catccacgat cgaccccacc accgcccgct cataccgcgg
      601 cggcgcgtcg gaggccaacg cactcttcac ggtgttcttc gaacacccca tcacccgcgc
      661 gatcaccttg atcggcatct tctccgcccg gtacaaccgg tggggtcggc atggagcgcg
      721 gtgtcgggat tgctcccgat ccgtttctcc atgccgccct cccgaaccgg acgtgcgagt
      781 tgtccccgca tccggctctc cacaagcctc gttggattgt ggctttcctc agttcacgct
      841 gggccacggt gaaggaattg tcgttccccg agggcggtag cgtgtggtga ctaccgatcc
      901 ggggttgaac agtacgattc ccccagcgcg cggccaccac gtgccgtcgc agtaacggcg
      961 gcgcaggtcc ttccaactcg tattggggtg tttgcgccgc agccacccga atacctgacg
     1021 ccagataacc atgcgcaggt attggaacgc tcgggcggac acccctggac ggaagtaagt
     1081 agtccagccc cggagcacca ggttgagctt gtgcaccagg accgccagcg gtaggccgac
     1141 gttgagccgg cagatcgcct tgcacttggc cttgattgac cgcagggcct tcttggccgg
     1201 gtagttatag acgtagtgcc ggttggttcc ccgcttctgg tgacgctgga tgcgccagcc
     1261 gaggaagtcg aggccctcgt cgatgtgggt gatcagcgtt ttgtcctccg acagccgcag
     1321 gccgacggtg gacaccacgg ccgctacttc gggaagcagc gcctcagcgt gcgctcgggt
     1381 cccggacacc aaaatcacga agtcgtccgc gtaccggatg agccggtagt tgggcagacc
     1441 tcgctggcgc cgtgtccacc gttgcttggt ggtggactgt ggtccgccgg gggccttggc
     1501 gaagtgttcg tcgaggaccg acagggccac attggccagc agcggtgaga gaatcccacc
     1561 ttgcggggtg ccggtaatgg tgtcttgggc ggtgcccctc tcggtgagga tgcccgcttt
     1621 caagaacgcc ttgaccagcg cgagcacgcg cttgtctccg attcttgctc gtacccggtc
     1681 catcagagcc ggatgcgaga tctcgtcgaa gcacgccgtg atgtcgccct ccagcaccca
     1741 ttcgtaagtc ttggatgcca gaaaacgggt ctcggccatg gcgtcgtgcg cccgccggtt
     1801 gggccgaaag ccatacgagc acggcacgaa gtccgcctcg aagatcggct ccagcaccag
     1861 cttcaaggac gcctgcacga cccggtcggc cacggtcggg atgcccaggc ggcgccgctt
     1921 gcgcgacccc ggcttgggaa tcatccgctc ccgcaccggc aacggctgga agctgccgga
     1981 cttcagctgc tcccgcagct cggccaggaa gatctccaca ccgatccgct cccggaccac
     2041 ggtgacggtc ctgccgtcga ccccagccga gcgtgcgccc ttgttccccg ctacccgctc
     2101 ccacgccatc aacagaaagc tgggatcggt cacgaggtta aacagatcat cgaaccgaca
     2161 atgaggatca tcattcgccc aacggtgcag cttggtctgg atctccagta cccgacgctc
     2221 agcccgaaac gagcccagcg gcagatcact cgtattcacc agcgctctcc tggtattcca
     2281 atacttccgc cactgacttg ctggctccct tcgccatgtg accggctttc ccggccacgg
     2341 actactacga agcctccgcc ccgcccggtg ccctcagcag acgacgcgcc tgcccttgcg
     2401 ccatgccggc agcacggagc gagggcgaca ccgaacggtt cccacgttca tctctaagtc
     2461 gatcgacggg tgaggcgtcc agctctaccc ctgcggcatc gccacggtta cgccgcagac
     2521 cttcaccgtg gcctcctggc cggtgatcac atcaggacca agagttcccc accaacaccg
     2581 aagcggtggg tgcgcaccgc gcccagccca tatccaccag atttgagctg gtgggccgtt
     2641 aaggggcgtc gaacactgat ttctctcgta caccttcccg tctcgctaac cgaacccgtg
     2701 tcatctggca gtaccgacac gtcccggcgt tgtcagggct gctcccaccc tccccggcgt
     2761 ctcccggatc aggctgcccc cagcttcacc cgaccgctgc gacggccaag gggcgaaggt
     2821 ctctcacctc cgctcgactt cagacgcctc gtggcgcaca tgggcctcgg cccagtcaat
     2881 gccgcaggtg atgctcatgt gcctccttga ccactgcctt cagggacaga gcccgagggt
     2941 gcgtgcggcg acctaatgga ggcgctccag gcgcggcatc tcaccagccg atcacggacc
     3001 cggcgactgg caggaccacc gtctatgttc aggcctcaac ggctcggtca agcatgggag
     3061 tggtcacctg tcagcggctc actcgacacc ctccaccaag cccgggccgg tgaaatctca
     3121 ggggcccctg ccagtcgcca ccgcgggacc ttgagggcct gcggccctcc cattaggtga
     3181 agtcgccgca ccagcgagtg ttgggtgccg cggcggtgaa gtcctgtctg atcaggtcgg
     3241 gagcatcgat aggccattga gctgcgatgg tggttttctt ccaggctcgc gggtgg
//
LOCUS       DATADN010000031         2169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1209836, whole genome shotgun sequence.
ACCESSION   DATADN010000031 DATADN010000000
VERSION     DATADN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209836"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..785
                     /locus_tag="VHH53_00390"
     CDS             252..785
                     /locus_tag="VHH53_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX2288675.1"
                     /translation="MLDRMKPAEEPRWLDPDERQTWLSVVSALVRLPATLDAQLQRDA
                     GISHFEYQVLAALSEAPDRTMRLSELAALAEGSLPRLSQVVSRLEKRGWVRRTPDPTD
                     GRYTLGILTEAGWDKVFATAPGHVEAVRRHVFDPLTKAQQRQLREITQRIMRTVDPDG
                     GCAPKLIRAPTRNRAEA"
     gene            972..1430
                     /locus_tag="VHH53_00395"
     CDS             972..1430
                     /locus_tag="VHH53_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6069 family protein"
                     /protein_id="HEX2288676.1"
                     /translation="MTSTATTVTIASTATRRAASWKSTAAAAGIGAAGGLVINSLIAW
                     AGRSLFDVPTAFQQLTPPVYGTLTVIGAIVGAVGWHLIATRSRNATRLLTWLVPTVLV
                     LSLIPDVMLLVDKSSQPGTTTAGVIALMLMHFGVAAAAVPAYRRFIPPQS"
     gene            1481..1816
                     /locus_tag="VHH53_00400"
     CDS             1481..1816
                     /locus_tag="VHH53_00400"
                     /inference="COORDINATES: protein motif:HMM:NF021496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HEX2288677.1"
                     /translation="MTTHLSRADVSTDAPARYAKQLVSHLGRKVAFATDGATSTATVG
                     NTTLAITVGETALTLTVTGPDPDGVTRAEHALGNHLERFGARRGLTVTWTRTTTTTAA
                     ATDGEGALR"
     gene            1813..>2169
                     /locus_tag="VHH53_00405"
     CDS             1813..>2169
                     /locus_tag="VHH53_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288678.1"
                     /translation="MTTTTARADALIPEAHPFRQHLHRIAALDTTSAQPHRAWTPADG
                     PLPSLYISHGAPPLFEDTAWMTQLHDWARALPKPKAILIVSAHWESAPLAISASRPDE
                     LVYDFGGFSPVYHQMRY"
BASE COUNT          375 a          844 c          634 g          316 t
ORIGIN      
        1 gccttgatgg ctgcgggcga ggactgctcg gtccagccca tgccggaggc ggccagcttc
       61 tccttgggct gcgtgagctt catcagcccg gcggcggcga acgcgacagc cagcagaccc
      121 gcgatgatcc acaatacgac gttcacaagg cctcctggcc gctcgatgac ttcatgtgtt
      181 aagtgaaacg tagcggctct tacttcaagc tgcaagtaat cgacagtgtc gttaacttca
      241 cgtgttaagt tatgcttgat cgcatgaaac ccgccgagga gccccgctgg ctcgacccgg
      301 acgaacggca gacctggctg tcggtggtca gcgcattggt ccggcttcct gccacgctag
      361 atgcccagct gcagcgcgac gccggaatca gccacttcga gtaccaggtg ctggccgcgc
      421 tgtccgaggc accggatcgc accatgcgac tgagcgagct ggccgcgctc gcggagggtt
      481 cactgccgcg gctgtcgcag gtcgtgagcc ggctggagaa acgcggctgg gtgcggcgca
      541 ccccggaccc gaccgacggc cgctacaccc tgggcatcct caccgaagcc ggctgggaca
      601 aggtgttcgc caccgccccc ggacacgtcg aagccgtccg ccgccacgtc ttcgacccgc
      661 tgaccaaggc gcagcagcga cagctccgcg agatcactca gcggatcatg cggaccgtcg
      721 accccgacgg cggctgtgca ccgaaactta tacgggcgcc caccaggaac cgggcagagg
      781 cgtagcaccc gtcctccgtc ccattccccg cccattgcca gcgatccgaa ccacattccc
      841 gccaccggga acggccaagg gcggcaacgt agtgacgcag cacacatccg aggttgttga
      901 agtttcaaca aaaggctccg atgcttgtta gtgaaagttc aacaacttgc cgagcgccac
      961 tggagaacac catgacgagc accgccacca ccgtcaccat tgcctcgacc gctacgcgcc
     1021 gtgcggcgtc gtggaagtcc accgccgccg cggccggtat cggcgctgcc ggcgggctgg
     1081 tcatcaacag cctcatcgcg tgggccggac gcagcctgtt cgacgtgccg accgcattcc
     1141 agcagttgac cccgccggtc tacggcacgc tcaccgtgat cggtgcgatc gtcggcgcgg
     1201 tcggctggca cctcatcgcc acccgcagcc gcaacgccac ccgcctgctc acctggctgg
     1261 tccccaccgt cctggtgctg tccctgattc cggacgtgat gctgctggtc gacaagtcct
     1321 cgcagccggg caccacgacc gccggcgtca tcgcgctgat gctcatgcat ttcggtgtcg
     1381 ccgccgccgc cgtgccggcc taccgccggt tcatcccgcc gcagtcctga ccgtcaccac
     1441 ggtccacaac cggccgcgac acatccgagg agcgttcgcc atgacgaccc acctgtcccg
     1501 cgcggacgtg tccaccgacg cgccggcccg ctacgccaag caactggtct cgcacctggg
     1561 ccgcaaggtt gcgttcgcca ccgacggcgc cacctcgacc gccaccgtcg gcaacaccac
     1621 cctcgcgatc accgtcggcg agaccgcgct gaccctcacc gtcaccgggc ccgaccccga
     1681 cggggtcacc cgcgccgagc acgccctcgg caaccacctc gaacgcttcg gcgcacgccg
     1741 gggcctcacc gtcacctgga cccgcaccac caccaccact gctgccgcga ccgacggcga
     1801 aggagcactg cgatgaccac caccaccgcc cgcgcggacg ctctgatccc cgaggcccac
     1861 ccgttccggc agcacctgca ccgcatcgcc gccctcgaca ccacctcggc gcagccgcac
     1921 cgggcgtgga cgccggccga cgggccactg ccgtccctgt acatcagcca cggcgcccca
     1981 cccctgttcg aggacaccgc ctggatgacc cagctacacg actgggcacg cgccctgccc
     2041 aagccgaaag cgatcctgat cgtcagcgcc cactgggagt cggcacccct ggcgatctcc
     2101 gccagccgcc ccgacgaact ggtctacgac tttggcggct tcagcccggt gtaccaccag
     2161 atgcgctac
//
LOCUS       DATADN010000032         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_908610, whole genome shotgun sequence.
ACCESSION   DATADN010000032 DATADN010000000
VERSION     DATADN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908610"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VHH53_00410"
     CDS             complement(<1..534)
                     /locus_tag="VHH53_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEX2288679.1"
                     /translation="MEVTGLLIAGLLIALLAIFTVVRAVRIVPQARARNVERLGRYQR
                     TLRPGLNFVIPYIDRVYPAIDLREQVVSFAPQPVITEDNLVVEIDTVLYFQVTDPRSA
                     AYEIASYLQAVEQLTVTTLRNVVGSMDLEGTLTSRDSINSQLRGVLDEATGRWGLRVN
                     RVEIKAIDPPKTIKDAME"
     gene            complement(537..968)
                     /locus_tag="VHH53_00415"
     CDS             complement(537..968)
                     /locus_tag="VHH53_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX2288680.1"
                     /translation="MAAWVIWLIIAVLLGVAEIFTLTAALGLLGGAAVIASVFAAIGL
                     PVPLQLLVFTLSAAAGIVILRPIATRHMLRPQLQRFGVEALVGRTAYVVREVTGRDGS
                     VRIGGEEWTARALDESLVIPAGATVDVIQIDGATAVVYPRE"
     gene            complement(1213..1374)
                     /locus_tag="VHH53_00420"
     CDS             complement(1213..1374)
                     /locus_tag="VHH53_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288681.1"
                     /translation="MTDRGRNRIGKLVTIGMRRDVRLAHRGRSGTGDCRCRAGNAHRA
                     SEQSEHEDQ"
BASE COUNT          276 a          534 c          534 g          267 t
ORIGIN      
        1 ctccatcgcg tccttgatcg tcttcggtgg gtcgatggcc ttgatctcca cccggttcac
       61 ccgcaggccc cacctgccgg tggcctcgtc aagcacgccg cggagctggc tgttgatgct
      121 gtcgcgggac gtgagcgtcc cttccaggtc catcgacccg acgacgttgc gcagcgtggt
      181 gacggtgagc tgctcgacgg cctgcaggta gctcgcgatc tcgtaggcgg ccgatcgtgg
      241 gtcggtcacc tggaagtaca gcacggtgtc gatctcgacg accaggttgt cctcggtgat
      301 gaccggctgc ggggcgaagg acaccacctg ctcgcgcagg tcgatcgccg ggtagacccg
      361 gtcgatgtag gggatcacaa agttcagccc aggccgcagc gtgcgttgat accggccgag
      421 ccgttccacg ttgcgcgctc gcgcctgtgg cacgatgcgc actgcccgca ctacggtgaa
      481 gatcgcgagc agcgcgatga gcagtcccgc gatcagcaaa cctgtgactt ccatggtcac
      541 tcccgtgggt agacgacggc ggtggcgccg tcgatctgga tgacgtcaac ggtggctccc
      601 gcgggaatca ccaggctttc gtccaaggcg cgggccgtcc actcctcacc gccgatgcgc
      661 acgctgccgt cccggcctgt cacctctcgt acgacgtacg ccgtcctacc gacgagcgcc
      721 tctaccccga agcgttggag ctgtggccgg agcatgtgcc gggtagcgat gggtcgcagg
      781 atcacgatgc cggccgcggc ggagagggtg aacaccagca gttgaagggg cacgggcagt
      841 ccgatcgccg cgaacaccga tgcgatcacg gcggcgccac cgagcaatcc caacgcggcg
      901 gtcagggtga agatctcagc gacgcccagc agcactgcga tgatcaacca gatgacccat
      961 gcagccacaa catcgaccct cctgaaccga ggtgacgcgc tcgccctcac ctatgggcga
     1021 gcgaagaacc ccagtcgatc gtaacctttc ggacagacgc aacgctgtgt tagccagggc
     1081 aggtcgcctt gttgccgtga ggtcaagaaa ctgcgatcaa cggcctctcc ggatcggccg
     1141 gtagggtcga agggccggtg agcagacagg cgggagggtc gagacgtgca ccgacacgcc
     1201 cgcccccgcg cgctactgat cctcgtgctc gctctgctcg ctggcgcggt gagcgttgcc
     1261 ggcccggcaa cggcagtcac cggtgccgct gcgcccgcgg tgagccagcc gaacgtcacg
     1321 ccgcatcccg atagtgacga gctttcctat gcgattgcgc ccgcgatccg tcacctcacc
     1381 gctccggcgc tattcggcct actaggcgaa gctgcaccgt caccaaccac ggatcagtcg
     1441 tctggtggcg cggccggtcc ctggggccgt ggatgcgcgc caccagacga ctgatccgtg
     1501 gttggtgacg gtgcagcttc gcctagtagg ccgaatagcg ccggagcggt gaggtgacgg
     1561 atcgcgggcg caatcgcata ggaaagctcg tcactatcgg gatgcggcgt g
//
LOCUS       DATADN010000033         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_154802, whole genome shotgun sequence.
ACCESSION   DATADN010000033 DATADN010000000
VERSION     DATADN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154802"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..789)
                     /locus_tag="VHH53_00425"
     CDS             complement(346..789)
                     /locus_tag="VHH53_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2288682.1"
                     /translation="MKVEEILKAKGRSVETIEPDASIAEAVGLLNGPPQIGALVIRSS
                     EGQRPIAGTLTERDIIRALGKYGSKLLTKRVTDVMSRNVPVCGPQDSIARLMQQMTAS
                     RYRHLPVVDRGKLVGLVSIGDVVKARVSDMELETGLLRDLYIARG"
     gene            complement(869..2959)
                     /gene="thrS"
                     /locus_tag="VHH53_00430"
     CDS             complement(869..2959)
                     /gene="thrS"
                     /locus_tag="VHH53_00430"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX2288683.1"
                     /translation="MSQLSPVSAEPAASVLVPGGTTAGQAVRKAGLPGKGADAVVVVR
                     DAESRLRDLSWVPETDTAVQPIAACTDAGRSVIRHSCAHVLAQAVQDQFPDAKLGIGP
                     PVKDGFYYDFDVDRAFTPEDLQALEKRMKQIIKQSQRFSRRVVESVEDAQQELAAEPY
                     KLELINLKSDVDTSEVMELGGSELTIYDNLDPRSGDRIWSDLCRGPHVPTTKHIPAFT
                     LTRVAAAYWRGNERNPQLQRIYGTAWESQEALDHHQAMLAEAERRDHRRLGTELDLFS
                     FPDEIGSGLAVFHPKGGIIRRELEDYSRRRHEESGYEFVYTPHITKSHLFEVSGHLDW
                     YAEAMFPGMHLDAELNADGTVRKPGQDYYLKPMNCPFHNLIYRSRGRSYRELPLRLFE
                     FGTVYRYEKSGVVHGLTRVRGLTMDDAHIYCTVEQVQAELQSLLSFVLGLLRDYGLED
                     FYIELSTRNDEKYIGTDEVWAEATETLRRAAETSGLDLVLDPGGAAFYGPKISVQAKD
                     ALGRNWQMSTFQVDLNLPDLFELEYTAADGSRQRPVMIHRALFGSIERFFGILTEHFA
                     GAFPAWLAPVQVVGIPIADEHAEHLHRVAKKLRAKGVRVDVDASDDRMQKKIRNHTTQ
                     KVPFMLLAGAKDVEAGAVSFRFRDGSQINGIPVDEAVAGVTEWIAQRVNSSPTADDFA
                     TETSSGGVDDRDLS"
     gene            3248..4048
                     /locus_tag="VHH53_00435"
     CDS             3248..4048
                     /locus_tag="VHH53_00435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2288684.1"
                     /translation="MLPADGEHLTTGWEPDLAVDDTLLRQAVLVHASWPVEVARGAGR
                     PWRSGPHWAGGWIGDRGALTNPVVLVQPLTRPEETVGDIGALFPPQVPYLLISAWPTP
                     NLRPYGLALLGHPPLMVRFPSPRPASPAPEVEVREVQDADELALAERVLVEGYPMPEL
                     EPLKPGDLLGPTLLQPATQVWLAWLDGAPVAVAAAHRHAGVTLVEYVATLPSVRGRGA
                     GAAVTWAATLAEPAAPAVLIASDDGRPVYERMGYVAVERWTAWLRPQR"
BASE COUNT          734 a         1392 c         1379 g          775 t
ORIGIN      
        1 atcgtcaccg acagcactgg tcgggccgga ccccagtccg ggcccccgac tgacaagctg
       61 gacaatcccg gagcgatatt ggaattttcc ttcgtagatt gataaggttg ctggacgagg
      121 ttacacgggg cagttcaggt cgccggcggg acctccatat ggcctagctg aacgaggatg
      181 gtggccaggt tatagtagat acttccgctg atgatctgcc cgctgcgttc aaccgcctaa
      241 tctcgcagaa ctgcgcgccg atcggcgtag ttggcgcggt acgctgcgct cggataggcc
      301 gggagatgtc aggacgctac tcgacgtaat gaaccgccag gcggactatc cgcgcgcgat
      361 gtaaagatcc cgcagcagcc ccgtctcgag ttccatgtcc gagacgcgcg ccttgacgac
      421 gtcaccgatg ctgaccaggc cgaccagctt gccccggtcg accacgggca ggtgacggta
      481 gcgggaggcg gtcatctgct gcatgagccg cgcgatgctg tcctgcggtc cgcagaccgg
      541 gacgttgcgc gacatgacgt cggtgacccg tttggtcaag agcttcgagc cgtacttgcc
      601 gagagcacga atgatgtcac gctcagtgag cgtgccagcg atcggccgct gcccttcgct
      661 gctgcggatg actagggccc caatctgagg aggcccattc agcaggccta ccgcctcagc
      721 gatgctcgcg tcgggctcga tggtctcgac cgatcgaccc ttcgccttca ggatctcctc
      781 gaccttcatc agtagtccct cctggtgacg ccggtcaccc catggtcctc ctgaaggcgg
      841 cggaccacca caggcatcca tgtgacgcct aactcaggtc acggtcatca actccacctg
      901 agctggtctc ggtggcgaag tcatcagcgg tgggggagga gttgacgcgc tgcgcgatcc
      961 actccgtgac gccggcgact gcctcgtcta ccggaatgcc attgatttga ctgccatcgc
     1021 ggaatcggaa ggacaccgca cctgcctcaa cgtcctttgc cccggccagc agcatgaacg
     1081 gaaccttttg ggtggtgtgg ttgcggatct tcttctgcat ccgatcgtcg gacgcgtcga
     1141 catcgacgcg gactcccttg gcgcgcagct tcttcgccac ccggtgcagg tgctcggcat
     1201 gctcgtccgc aatcggaata ccgaccacct gcaccggagc gagccacgcc gggaaagccc
     1261 cggcgaagtg ctcggtgaga atgccgaaga accgttcgat tgagccgaaa agcgcccggt
     1321 gaatcatgac cggtcgctgc cgcgagccgt cagcggcggt gtactccagt tcgaacagat
     1381 cgggcaggtt cagatccact tgaaaggtcg acatctgcca gtttcggccc agtgcgtcct
     1441 tggcctgcac cgagatcttg ggaccataga acgcggcgcc acccgggtcg agcaccaagt
     1501 ccagcccgct ggtctcagcg gcccggcgca aggtctcggt ggcctcagcc cagacctcgt
     1561 cagtgccgat gtacttctcg tcatttcggg tcgacagctc gatgtagaaa tcctccagcc
     1621 cgtagtcccg cagcaggccg agcacgaacg acagcagcga ctgcagctcc gcctgcacct
     1681 gctccacggt gcagtagatg tgcgcgtcgt ccatggtcag gccccggacc cgagtcaggc
     1741 cgtgcaccac accggatttc tcgtagcgat ataccgtgcc gaactcgaac agccgtagcg
     1801 gcagttcccg ataagatctc ccgcgcgacc tgtaaataag gttgtgaaac gggcagttca
     1861 tcggcttcag gtagtaatcc tggccgggct tacggacggt gccgtcggcg ttgagttcag
     1921 cgtccaagtg catgccggga aacattgcct cggcatacca gtccagatgg ccggatacct
     1981 cgaacagatg ggacttggtg atgtgcgggg tatagacgaa ctcataaccc gactcctcgt
     2041 gacggcgtcg tgagtagtcc tccagctcac gccggatgat gccgcccttg ggatggaaca
     2101 ccgcaagacc ggagccgatc tcgtccggaa aactgaacag gtccagctcg gtaccgaggc
     2161 ggcgatggtc acggcgctcg gcttcggcga gcatcgcctg gtggtgatcg agggcctcct
     2221 gcgactccca cgccgttccg tagatgcgtt gcagttgggg gttgcgctcg ttgccccgcc
     2281 aatacgccgc cgcgacgcgg gtcagggtga aggcggggat gtgcttggtc gtcggcacat
     2341 gcgggccgcg acacagatca ctccagatcc ggtccccgct gcgtgggtcg agattgtcgt
     2401 agatggtcaa ctcgctgccg ccgagctcca tcacctcgga ggtgtccacg tcggacttca
     2461 agttgatcag ttccaacttg tacggctcgg ccgccagctc ctgctgtgcg tcctcgaccg
     2521 attccacgac cctgcgagag aaccgctggg actgcttgat gatctgcttc atccgctttt
     2581 ccagcgcctg caggtcctcg ggggtgaacg cccggtcgac atcaaagtcg tagtagaagc
     2641 cgtctttcac cggcggcccg atgccgagtt tggcgtccgg gaactgatcc tgaacggcct
     2701 gcgccagcac gtgggcgcag gaatgccgga tgacactgcg gcccgcgtca gtgcaggccg
     2761 cgatcggctg cactgccgtg tcggtctctg gcacccagga cagatcacgc agtctgctct
     2821 cggcgtcccg cacaaccacc acggcgtcgg cgcccttgcc gggcaagccc gccttccgca
     2881 ccgcctggcc agctgtggtc ccgcccggca ccagcacgct cgcggcgggc tcggcggaca
     2941 cgggactgag ctgggacaca gtggctcctt gttgatcagg ttcggtaatc agggggtctg
     3001 gcgaccgatg ctatcctgtc ggatccggca ctctctcctc agttaccgcc cctgcgagga
     3061 cgacttcgat ggcgaccgtg tcaggcgcac ccgaagaccg gagggtgact agcacgacgg
     3121 ggagcgtgta cgtgccacag ccgtcgatct ctcataaacg cgttacctaa gaatcaaaac
     3181 tctagaccgg tattgggtat tgctgcgctg cggacctgcc gtcatcgtgg cgagcatgag
     3241 tacgtcgatg ctcccagcgg atggcgagca cctcacgacg ggttgggagc cggatctcgc
     3301 ggtcgacgac actctgctcc ggcaggccgt cctcgtgcac gcctcgtggc cggtggaagt
     3361 cgcgcgcggc gcgggacgac cgtggcgatc cgggccgcat tgggccgggg gctggatcgg
     3421 cgaccgcgga gcgctgacca atccggttgt tctcgtacag ccgttgaccc gccccgaaga
     3481 aaccgtgggt gatatcggtg ctctgttccc gccgcaggtg ccttacctgc tgatcagcgc
     3541 gtggcccacg ccgaacctac ggccctacgg actcgccctg ctcgggcatc cgccgctgat
     3601 ggttcgtttc cccagcccgc ggccggcctc gcccgcgccg gaagtcgaag tgcgcgaggt
     3661 ccaggacgcg gacgagctcg cgctcgccga gcgggtcctc gtcgagggtt acccgatgcc
     3721 ggagctggag ccgctcaagc cgggcgacct gctcggtccc acgctgctgc agccggctac
     3781 ccaggtatgg ctcgcgtggc tggatggtgc accggtagcg gtggcggcag cacaccggca
     3841 cgccggggtt acactcgtcg agtacgtcgc aacgcttccg tcggtgcgag gccgcggtgc
     3901 gggcgccgcg gtgacctggg ctgccaccct cgccgagccg gcagcgccgg cggtgctcat
     3961 cgccagcgac gacggtcggc ctgtgtacga acgaatgggt tacgtcgccg tggagcgctg
     4021 gaccgcctgg ctccgcccgc agcggtaggt ccaggcgtat ctggttgtgc tcatccgctc
     4081 tcccttaagc acgcataccg cggaacccga ccgcaggagg ctgaaaacat gactgtggaa
     4141 gctaccggcg cggcgcccac ggaggtgggc aacggtctcg ctcgggaact gatgtccagg
     4201 ctgcgtgggc agctcgtccg gcctgcagac gacggttacg acaaggcccg tctggtgtgg
     4261 aacggcatgg tggacaagcg
//
LOCUS       DATADN010000034         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1059481, whole genome shotgun sequence.
ACCESSION   DATADN010000034 DATADN010000000
VERSION     DATADN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059481"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..610)
                     /locus_tag="VHH53_00440"
     CDS             complement(239..610)
                     /locus_tag="VHH53_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288685.1"
                     /translation="MARAAVKEMARQVGPLVVDSDGLARRGLLLRHLVMPGLLVETEA
                     VLRFVAEELGRDTYLDLMAQYYPAGLVGKDGRDGYGEIDRHLYREEYLEAVDLGHHLG
                     LRLDVRSVASAARLAPADATT"
     gene            complement(617..1222)
                     /locus_tag="VHH53_00445"
     CDS             complement(617..1222)
                     /locus_tag="VHH53_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2288686.1"
                     /translation="MAMAWDGTPSYLRLSTDELAGRARQALALLGQDRCGVCPRLCKV
                     DRLADHRGLCAIGRQAVVASYFAHFGEENCLRGWNGSGTIFFSGCNLRCVFCQNHDIS
                     WEVQGERVIPARLAEMMLALQARGCHNINWVTPEHVVPQILEAVPLAVAGGLRLPIVY
                     NTSAYDSLDSLQLMDGIVDVYMPDFKVWSRPTARRYLRRPD"
     gene            complement(1261..>1643)
                     /locus_tag="VHH53_00450"
     CDS             complement(1261..>1643)
                     /locus_tag="VHH53_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HEX2288687.1"
                     /translation="APVTIERGIDEVGADDFDALVIPGGYSPDKIRTSPGMVALTRDM
                     HQALKPVAAICHAGWMLAEADIVRGKTVTSYHSIKTDLRNAGADWVDQELVEAVSDAG
                     GIIITSRHPGDLPAFCEALLRQLK"
BASE COUNT          254 a          590 c          551 g          248 t
ORIGIN      
        1 aaggcggtcc gcacccaggg cgccggccgc cgctataccg ccctcactca gcggctgcgg
       61 cccggaccgg tcggagccag cgccgcagtc aactgatcca ggggctgcca gcggcttcca
      121 gccattcctt gtgggtgggc cctccggctc cagcctcatc gggacgtcgc ggccgcaccg
      181 gccaggtagc gttgcgcagg ccggttccag acaagcatct gatacccgct cggcagcgtc
      241 aggtcgtggc gtcggcggga gccagccggg ccgcgctggc gacgctgcgg acgtccagcc
      301 gcagccctag gtggtggccg aggtcgaccg cctccaggta ctcctcccgg tacaggtgcc
      361 ggtcgatctc gccatagccg tcccggccgt ccttgccgac caggccggcc gggtagtact
      421 gggccatcag gtccaaatag gtgtcccggc ccagctcctc ggccacaaac cgcagcaccg
      481 cctcggtctc gaccagcagg ccgggcatca ccagatgccg cagcagcagc ccccggcggg
      541 ccaggccgtc agagtcgacc accagcgggc cgacctgccg agccatctcc ttgacggccg
      601 ctctggccac ctccgcttag tctgggcgcc gcagataccg gcgggccgtc ggccggctcc
      661 agaccttgaa gtcgggcatg tagacgtcga cgattccgtc catgagctgc aggctgtcca
      721 ggctgtcgta ggcgctggtg ttgtagacga tcggcaaccg cagccccccg gcgacagcga
      781 gggggacggc ctccaggatc tgggggacga catgctcggg cgtgacccag ttgatgttgt
      841 gacagccccg ggcctgcagg gccagcatca tctcggccag gcgcgctggg atcacccgct
      901 cgccctggac ctcccaggag atgtcgtggt tctggcagaa cacacagcgc aggttgcagc
      961 cggagaaaaa gatcgtcccc gagccgttcc agccccgcag gcagttctcc tccccgaagt
     1021 gcgcaaagta ggaggcgacc acggcctggc ggccgatggc gcacagtcct ctgtggtcgg
     1081 ccaggcggtc caccttgcac aggcgcgggc agaccccaca gcgatcctgc ccaagcaggg
     1141 cgagagcctg tcgggctcgg ccggccagct cgtcggtgct cagccgcaga tacgacggcg
     1201 tcccgtccca agccatcgcc atcgcaaccg tcctctcgat ccgcgctcgc gggtcagccg
     1261 ttacttgagc tggcgcaaca gcgcctcgca gaaggcgggc aggtcgccag ggtggcggga
     1321 ggtgatgatg atgccgccgg cgtccgagac cgcctccacg agctcctggt cgacccagtc
     1381 cgcgccggca ttccgcaggt cggtcttgat cgagtggtag gaggtcacgg tcttgccacg
     1441 cacgatgtcg gcctcggcca gcatccatcc ggcatggcag atggccgcca ccggcttgag
     1501 cgcctggtgc atgtcgcggg tcagggccac catccctggg ctggtccgga tcttgtccgg
     1561 cgaatagcca cccgggatca ccagcgcgtc gaagtcgtct gcgccgacct catcgatgcc
     1621 gcgttcgatg gtcactgggg cgc
//
LOCUS       DATADN010000035         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_758087, whole genome shotgun sequence.
ACCESSION   DATADN010000035 DATADN010000000
VERSION     DATADN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758087"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VHH53_00455"
     CDS             complement(<1..303)
                     /locus_tag="VHH53_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX2288688.1"
                     /translation="MFDTLSDRLTSALSDLRGKGRLSDADIDATAREIRIALLEADVA
                     LPVVRQFIAHVKERAKGVEVSAALNPAQQVIKIVNDELIGILGGETRRLELAKTPPT"
     gene            complement(617..1537)
                     /locus_tag="VHH53_00460"
     CDS             complement(617..1537)
                     /locus_tag="VHH53_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288689.1"
                     /translation="MSAQLRSRRMYRTRDEAVPVNPPFEIVLRGFDRQQVIDHVEALK
                     TRVATIGAERDAALQRAADLSEQFENLRREAVEATTQVERLRREATAAASQVDRLERS
                     PLTAATARIQRMLQMAEDEATELQNSAEQETTSLRESARAEADALLHETSQRCMRLEA
                     ESERRRQTAEAESTARCQQVELDAERRASAAEQRSEHNLARRRAETEAWISDYQTRSM
                     AALYLIVRLAVQGLTSRMAEAERQASALKNLRAEVADQVFAVHRLMVEAVGLVDQPTA
                     AEDIDSAEYVEAVEAVPAADQPTARFGLHP"
     gene            complement(1534..>2064)
                     /locus_tag="VHH53_00465"
     CDS             complement(1534..>2064)
                     /locus_tag="VHH53_00465"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104455.1"
                     /note="catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; also involved in
                     adenylylating and deadelnylyating GlnK; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX2288690.1"
                     /translation="GVLALHSLQVHSADIRVVELPAGGSAAVDIFTVSPRFGRLPDVA
                     LLRQDLLRAMEGSLQLADRLVAKERDYGEAPRCETTASETGSADVDTRVLWFDDEATA
                     AVILELRTTNHRIGLLYRVAAALESSAVTVRWARVVTLGSSVVDSFCITGSDGCSVLS
                     PKARALVERAVLNAAR"
BASE COUNT          326 a          729 c          663 g          346 t
ORIGIN      
        1 ggtcgggggc gtcttggcca gttccaggcg ccgggtctcc ccgcccagaa tgccaatgag
       61 ctcgtcgttg acgatcttga tgacctgctg ggcggggttc agcgcagcgg acacctccac
      121 acccttggcc cgctccttga cgtgcgcgat gaactggcga accaccggca gcgcgacatc
      181 ggcctcaagc agcgcgatgc ggatctcgcg ggcggtggca tcgatgtcag catcggagag
      241 cctgcctttg ccccgcagat cggacagcgc tgacgtgagc cggtcggaga gggtgtcgaa
      301 cacgccggtc gcgctccctt agagtccggc ggggtgaggt gccccggagg ctggctgtag
      361 tactggtagt actgccagcg tagcggcccg ccgccgggac aagagcctgg accgccggcg
      421 cccgagcccc accccaggct gccggcggtc cggtaatgac cgttgcgatc accagcgaca
      481 cacccgccac ccgactcgcc agtacgggcg gcagccccgc gtctgcccga ccgctgccga
      541 gccacccgac cagcctggca atcgcgagta ccgccggcta gcgtaaatct ccgcgatcgc
      601 gactgggtga aactacctac ggatgcaggc cgaaccgtgc tgtgggctga tccgctgccg
      661 gtacagcctc cactgcctcc acatactccg cagagtcgat gtcctcggct gcggtgggct
      721 gatcgaccag gccgaccgcc tccaccatca ggcggtgcac cgcgaacacc tgatcagcga
      781 cctcggctcg caggttcttg agtgcgctgg cctggcgctc ggcttcggcc atccggctgg
      841 tcaacccctg caccgccaga cgcacgatca agtacagcgc agccatgctt cgggtctgat
      901 aatcgctgat ccacgcttcc gtctcggcgc ggcgccgagc gaggttgtgc tcggatcgct
      961 gctcggcagc cgacgcacga cgttcggcgt ccagctccac ttgctggcac cgggctgtgg
     1021 attcggcctc tgcggtctga cgccggcgct ccgattccgc ctccagcctc atgcaccgct
     1081 ggctggtctc gtgcagcagc gcatcggcct cggcgcgagc gctctcgcgc agcgacgtgg
     1141 tctcctgctc ggcactgttc tgcagttcgg tggcctcgtc ctcggccatc tgcagcatgc
     1201 gctggattcg cgcggtcgct gcggtcagcg gggaccgctc cagacgatcg acctggctgg
     1261 ctgccgccgt cgcttcccgg cgtagccgct cgacctgagt ggtcgcctcg actgcttcgc
     1321 ggcgcagatt ctcgaattgc tcgctgagat cagcggcccg ctgcagcgca gcatcgcgtt
     1381 cggccccgat cgtcgcgaca cgagttttga gggcttcgac gtgatcaatg acctgctgac
     1441 ggtcgaaccc gcgcaacacg atctcgaacg gcgggttgac cgggacggcc tcatcgcgag
     1501 ttcggtacat gcggcgggat cgtagctggg cgctcaccgc gcggcattga gcactgcccg
     1561 ttccaccaac gcgcgcgcct tcggcgacaa cacactgcaa ccatcggagc cggtgatgca
     1621 gaacgagtcc actactgaac tgcccagcgt gacgacccgg gcccagcgaa cagtcaccgc
     1681 actgctctcc aacgccgcgg caacccggta cagcaacccg atgcggtggt tggtggtgcg
     1741 aagctcgagg atcaccgctg cggtggcctc gtcgtcaaac cacagcaccc tggtgtcgac
     1801 gtcggcggat cccgtttcgg acgctgtggt ttcgcacctt ggggcctcgc catagtcgcg
     1861 ctccttggcg accagccggt cggccaactg cagcgagccc tccatcgccc ttagcagatc
     1921 ttggcgcagc agcgcgacat cgggcaggcg tccgaagcgc ggtgagacag tgaaaatgtc
     1981 caccgccgca gagcctccag cgggcagttc gacaacccgg atgtccgcgg agtggacctg
     2041 cagcgagtgc agcgcgagta cccc
//
LOCUS       DATADN010000036         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_456740, whole genome shotgun sequence.
ACCESSION   DATADN010000036 DATADN010000000
VERSION     DATADN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456740"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VHH53_00470"
     CDS             complement(<1..266)
                     /locus_tag="VHH53_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2288691.1"
                     /translation="MSTNDGPGVPPERGPEQTSIFRADFLAEPESVEAPGPPIAGVDA
                     LPAGSALLVVKRGPNAGSRFLLDRSTTSAGRHPDSDIFLDDVTVS"
     gene            complement(369..953)
                     /locus_tag="VHH53_00475"
     CDS             complement(369..953)
                     /locus_tag="VHH53_00475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2288692.1"
                     /translation="MDPADRLFTVPNVLSLLRLAGVPLFLWLLLGPQYDLLAMLVLAL
                     SGITDWLDGKLARLLNQSSRLGELLDPAVDRLYTLTTLLAFGVRDILPWWVIGVLIGR
                     DVVLSLTLPILRHHGYGPLPVHYLGKAATLCLLYAFPFLLLADNVQGWLQVVAGAFAL
                     AFTGWGGALYLWSGVLYLIQVLLTVRAARRAHRS"
     gene            complement(965..>1535)
                     /locus_tag="VHH53_00480"
     CDS             complement(965..>1535)
                     /locus_tag="VHH53_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX2288693.1"
                     /translation="LDVAAAPEDLDEVGGWLRDGAGALVDAIRTAGPQTPVWTWSDDQ
                     RAGFWARRMAHETAVHRADAELALGREFELEPDLAADAISEWLGALSSPQAVEFRPEL
                     AELRGEGEILHLHSTDPGLGEAGEWIVRRTPSGPVWEHGHSKGDVAVRGAVVDLLLML
                     TRRIPAEQAPIEVLGDEALLVHWLQHTRF"
BASE COUNT          276 a          549 c          498 g          212 t
ORIGIN      
        1 gacaccgtca cgtcgtcgag gaagatgtca ctgtccgggt gccgacccgc gctggtcgtg
       61 gaccggtcga gaaggaaccg agaaccggcg ttcggaccgc gtttgaccac cagcagtgcc
      121 gagcccgcgg gtaacgcgtc gacacctgcg atgggtggcc ccggtgcctc gacactctcg
      181 ggctcggcga ggaagtcggc ccggaatatc gaggtctgct ccgggccccg ctccggcgga
      241 acgccgggcc cgtcgttcgt gctcacctga gctctccttc accaccggtg gtgcctgccg
      301 atcggatcac aacctacctg ccaccggcga ccggtgcgga ttgaggtccc gcccgccgcg
      361 gcccgacgtc agctccggtg tgcccgtctc gcggcgcgca ctgtgagtag cacctggatc
      421 aggtagagca ctcccgacca caggtacagc gcgccacccc atccggtgaa ggccagcgcg
      481 aatgcaccgg ctacgacctg taaccaaccc tgcacgttgt cggcgagtag caggaacgga
      541 aaggcataga gcaggcacag cgtggctgcc ttgcccagat agtgcaccgg cagcgggccg
      601 taaccatgat gacgcagtat cggcagggtc agcgagagca ccacatcccg gccgatcaac
      661 acgccgatca cccaccaggg caggatgtcg cgcaccccga aagcgagcag ggtggtcagg
      721 gtgtagagcc ggtcgaccgc cggatcgagc aactcgccca gcctgctgga ttggttcagc
      781 aagcgggcca gcttgccgtc caaccagtcc gtgatgcccg acagagctag taccagcatg
      841 gcgagcaggt cgtactgcgg gcccagcagc agccagagga acagcgggac accggccaac
      901 cgcagcaggc tcagcacatt gggcactgtg aacagccggt cggccggatc cataccggcc
      961 gaccctagaa ccgggtgtgc tgcagccagt gcaccaacaa tgcctcgtcg ccgagcacct
     1021 cgatcggcgc ctgctcggct ggtatccggc gggttagcat caacagcagg tctaccaccg
     1081 caccgcggac ggcgacatcg cccttgctgt ggccgtgctc ccagaccggc cccgacggcg
     1141 tcctgcgcac gatccactca cccgcctcgc cgagcccggg gtcggtggag tgcaggtgca
     1201 gaatctcgcc ctcgccgcgc agctcggcga gctcgggtcg aaactcgacc gcctgcggcg
     1261 agctgagagc gcccagccac tcggagatcg catcagcagc gagatcgggc tccagctcaa
     1321 actcgcggcc cagcgccagc tcggcgtcgg ctcggtgcac tgcagtctca tgtgccatcc
     1381 ggcgggccca gaacccggcg cgttggtcgt cagaccaagt ccacaccggg gtctgcgggc
     1441 cggcggtccg gatcgcgtcg accagtgcac cggcgccgtc gcgcaaccag ccaccgacct
     1501 cgtcgaggtc ctcgggcgct gcggccacat ccagc
//
LOCUS       DATADN010000037         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_155098, whole genome shotgun sequence.
ACCESSION   DATADN010000037 DATADN010000000
VERSION     DATADN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155098"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..951
                     /locus_tag="VHH53_00485"
     CDS             364..951
                     /locus_tag="VHH53_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX2288694.1"
                     /translation="MSAPPVSAPPVYALGAYRGRLRTALLAYKEHGRRDLAASLGGAL
                     AAALLQLAPVTGTGTGSESGGVCLVPVPSRRSAAARRGGQHVTLLAQQAAAALANAGM
                     AASVAPALRLAAGVRDSVGLHAAARQANLAGRVLLRRSGLPPIGMLVVLIDDVVTTGT
                     TVAACVAALVRAGIDTPAIVVLAAAGYHWPSYLKP"
     gene            1192..>1820
                     /gene="raiA"
                     /locus_tag="VHH53_00490"
     CDS             1192..>1820
                     /gene="raiA"
                     /locus_tag="VHH53_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX2288695.1"
                     /translation="MNIVVKGRNVEVPDHYRVHVAEKLSRVERYDHKAIRYDVELFHE
                     RNPRQSKSCQRVEITGQCRGQVVRAEASGPDFYAALDAATSKLANRLRRVADRRKVHH
                     GRRQPTSVAEATAPLAAVLAPDIEADVESGPESNGQRHRTAVLDAPDHELEYELPTQR
                     WHSPDADGLGRIVRRKKHSAEPITVDQALYEMELVGHDFYLFSDADSGLP"
BASE COUNT          281 a          615 c          614 g          310 t
ORIGIN      
        1 cgtattcgga cactcaagag gtgtgggaac cgctgctcgg cggcatcgga cccggcgcgg
       61 tacccctata ccctggctga ccggctggcg caagtccggc tgctggggct ggggacagcc
      121 gctctgctgg gtacagccgg gccactgacg gagccgtcag ccggtggcgg cgacaccagg
      181 gtccatgctg gctgggtggc tggatggacg catgcgctgc tggacctgat gttgcccgtc
      241 gaatgtgccg gctgcggggc gccgagcact ccctggtgcc ccagctgcgc ggggctgctg
      301 gggtcaccga tgcaggtaca tccgccgcac ttgtcggccg gtctcggagt ttctgcaaca
      361 ccagtgtctg ccccaccggt gtctgcccca ccggtctatg cactaggcgc gtaccgcggt
      421 cggctacgca ccgcactgct ggcctacaag gagcacggtc gccgcgatct cgcggcttcg
      481 ctgggtggcg cgctcgctgc cgcgctgtta cagctggccc ccgtaaccgg cactggaacc
      541 ggatccgaat ccggcggggt atgcctggtg ccggtgccgt cgcgccgatc ggccgcggct
      601 cgccgcggcg ggcagcacgt gacgctgctg gcgcagcaag cggctgctgc gctggccaac
      661 gcagggatgg cggcctcggt cgctccagcg ttgcggttgg cggccggagt acgcgactcc
      721 gtcggtctgc atgccgcggc tcggcaggcc aacctggccg gacgagtgct gttgcgacgg
      781 agcgggctgc cgcccatcgg tatgctcgtc gtgctcatcg acgacgtggt gaccaccggc
      841 acaaccgttg ctgcctgcgt cgcagcgttg gtccgggcgg gtatcgacac gcccgcgatc
      901 gtggtgctcg ctgccgcagg ttatcactgg ccgtcgtatc tcaagcccta gaacaccact
      961 cacagtgttg aggatccgga tcgacccgtc aggccctggc gacggcacct ccggcggact
     1021 accgttcggc catcagcact gacgctgctg atgcgaaggg aggcgagaca gccatgattt
     1081 tggcgccggc gccctagtga tcacgcggat ggcccccctt gccgttcgag catcaccgca
     1141 gtgccccggc gtcgccgaag tcctgctgga caacgcgagg aggtcgcacg cgtgaacatt
     1201 gtcgtgaagg gtcgtaacgt ggaggtgccc gatcattatc gggtgcacgt cgccgagaaa
     1261 ctctcccgcg tcgaacgtta tgaccacaag gccatccgtt atgacgtcga gctttttcac
     1321 gaacgcaatc ctcggcagtc caagagctgc cagcgagtgg agatcaccgg tcaatgtcgc
     1381 gggcaggtag tccgcgctga agcgtccggt cccgacttct acgccgcctt ggacgcggcc
     1441 accagcaagc tggcgaaccg gctgcgccgg gtagctgatc gtcgcaaggt gcaccatgga
     1501 cgtcgtcagc ccacgtccgt cgctgaggcc accgcgccgc tggcggccgt cctggccccc
     1561 gatatcgagg cagatgtcga gagcgggcct gaatccaatg gtcagcgcca ccggacggcg
     1621 gtgctcgacg cacccgacca cgaactcgag tacgagctgc ccactcagcg gtggcattcc
     1681 ccggatgcgg acggactggg tcgcatcgtg cggcgcaaga agcactccgc cgaacccatc
     1741 accgtcgatc aggcgctcta cgagatggag ctcgtgggcc acgacttcta cctgttttcc
     1801 gacgccgact ctggcctgcc
//
LOCUS       DATADN010000038         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_306136, whole genome shotgun sequence.
ACCESSION   DATADN010000038 DATADN010000000
VERSION     DATADN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306136"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1767)
                     /locus_tag="VHH53_00495"
     CDS             complement(<1..>1767)
                     /locus_tag="VHH53_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX2288696.1"
                     /translation="LLADKQEGYHRLCRTLTNGHLAGYPDSSTRPVGPAEKGRPVYDL
                     DQVVAETREHCVVLTGCRKGAVRRALVHNGPEAAFVQLGKLVGAYGRDRVFVELTDHG
                     QPADSVHNDLLYGMANDLGLPCVATNAVHYAHPERGRLAAAMAAVRAGRGLDEMDGWL
                     PPAPTAHLRSGEEMHRRFARYPGAVQRAAVLGMQLQFPLALIAPQLPPFGVPPGQTEI
                     TYLRELTYRGAARRYGSYSESPRAFAQLHHELQVIEELGYPGYFLVVWEIVDFCRRNN
                     ILCQGRGSAANSAVCYALGITNVDAVRYELLFERFLAPARDGPPDIDVDIESDRREEV
                     IQHVYDEHGRRHAAQVANVISYRARSAVRDMAKALGYSPGQQDAWSKQIDRWGPLKST
                     MDDNDHDVPLTVLELAGQLENFPRHLGIHSGGMVICDRPVSEVCPVEPARMPKRTVLQ
                     WDKDDCAAVGLVKFDLLGLGMLSALHYVIDLVAEHYGRTVDIGELDLCDEKVYAMLQR
                     ADSVGVFQVESRAQMATLPRLKPKEFYDLVVEVALIRPGPIQGGSVHPYIRRRNGVET
                     VTYLHPLLERSLAKTLGVPLFQ"
BASE COUNT          259 a          631 c          607 g          270 t
ORIGIN      
        1 cctggaacaa gggcacgccc agggtcttgg ccaggctgcg ctccaacagg gggtgcaggt
       61 aggtgacggt ttccacgccg ttgcgccggc ggatgtaggg gtgcaccgag ccgccctgga
      121 tcgggcccgg ccggatcagc gcgacctcga cgaccaggtc gtagaactcc ttgggcttca
      181 accgcggcag cgtggccatc tgcgcgcggc tctccacttg gaacacaccg accgaatcgg
      241 cccgctgcag catcgcgtag accttctcgt cgcacaggtc cagctcgccg atgtccaccg
      301 tgcggccgta gtgctcggcc acgaggtcga tcacgtagtg cagggcggag agcatgccca
      361 ggccgagcag gtcgaacttc accagcccca ccgcggcgca gtcgtccttg tcccactgca
      421 gcacggtccg tttcggcatc cgcgccggtt ccaccgggca gacctcgctc accggccggt
      481 cgcagatcac catgccgccg gagtggatcc ccagatgccg agggaagttc tccagctgcc
      541 cggccagctc gagcacggtg agcggcacgt cgtggtcgtt gtcgtccatc gtggacttca
      601 gcggacccca ccggtcgatc tgcttgctcc aggcgtcctg ctggcccggc gagtaaccga
      661 gcgccttggc catgtcccgg accgcggacc gggcccggta gctgatcacg ttggccacct
      721 gggcggcgtg ccggcgcccg tgttcgtcgt agacgtgctg gatgacctcc tcgcgccggt
      781 cggactcgat gtccacgtcg atgtccggcg ggccgtcccg ggccggcgcg aggaaccgct
      841 cgaacagcag ctcgtagcgc acggcgtcga cgttggtgat gcccagcgcg tagcagaccg
      901 ccgagttcgc cgccgaaccc cggccctggc acaggatgtt gttacgccgg cagaagtcca
      961 cgatctccca caccacgagg aagtagcccg gatagccgag ctcctcgatc acctgcagct
     1021 cgtggtggag ctgggcgaac gccctcgggc tctcgctgta cgagccgtag cgcctggccg
     1081 cgccgcggta ggtcagctcg cgcaggtatg tgatctcggt ctggccgggt ggcacgccga
     1141 acggcggcag ctgcggtgcg atgagcgcca acggaaactg cagctgcatg ccgagcaccg
     1201 cggcccgctg cacggcaccg gggtagcggg cgaaccggcg gtgcatctcc tcaccggagc
     1261 gcaggtgcgc ggtgggcgcc ggcggcagcc agccgtccat ctcgtccagc ccgcggccgg
     1321 cccgcaccgc ggccatcgcg gcggccaggc ggccccgctc cggatgcgcg tagtgcacgg
     1381 cattggttgc cacacaaggc agtcccaggt cgtttgccat gccgtagagc aggtcgttgt
     1441 gtaccgagtc cgccggctgg ccgtgatcgg tcagctcgac gaacacccgg tcccggccgt
     1501 aggcgccgac cagcttgccc agctgcacga acgccgcctc cggtccattg tggacaagcg
     1561 cgcggcgaac ggcgcccttg cggcacccgg tgagcaccac gcagtgctcc ctggtctcgg
     1621 cgaccacctg gtcgaggtcg tagaccgggc gccccttctc ggccggaccc accggccggg
     1681 tcgagctgtc cggatagccg gccaggtggc cgttggtgag cgtgcggcac agccggtggt
     1741 agccctcctg cttgtcggcc agcagca
//
LOCUS       DATADN010000039         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_4516, whole genome shotgun sequence.
ACCESSION   DATADN010000039 DATADN010000000
VERSION     DATADN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4516"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1193
                     /locus_tag="VHH53_00500"
     CDS             144..1193
                     /locus_tag="VHH53_00500"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX2288697.1"
                     /translation="MESSQVMLINLYSDTQTRPVDDMRAAMAAAEVGDEQRGEDPTVN
                     MLCERVATLLGKPAAVFLPSGTMCNAISFRLHIRPGGDAVHLHPTSHPIVAEAGGPSA
                     LSGTVLAPVPGEAGMFTAEALEAALFPAGDRYEPRSRLVSVEQTTNLAGGRVWPLEQM
                     RAVLEIAARHRLRAHLDGARLMNAVVASGVPAAEWARGFDTAWIDFTKGLGAPVGAVL
                     AGSTELIEEAWRYKQMMGGALRQAGIVAAGCLWALDHHVDRLADDHANARILAAGLAG
                     LPGITLDPDTVETNIVVFGLADAPRFCQRLEAEHGVRMGALGPDAVRAVTHHDVDTAD
                     IHSAVHAVAAVRNPA"
     gene            complement(1282..>1708)
                     /locus_tag="VHH53_00505"
     CDS             complement(1282..>1708)
                     /locus_tag="VHH53_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HEX2288698.1"
                     /translation="APDTFDASGYAALLRRLREDSDEVVYAPSFQRSLEQPIAGAIPI
                     PPCTRLIITEGNYLLLHEHGWGRVRAQLDEVWYVDLDADERVRRLIARHVRFGKQPAV
                     AAAWATGTDERNAVLISSTRSHADLIVPATLMRSLTAPP"
BASE COUNT          253 a          555 c          624 g          276 t
ORIGIN      
        1 ctcgcggctg gcctcgccgc cgacctgctc gtcctcgccg gcaacccact tcatgacatc
       61 gcagccttgg atcaactgga attggtggtc gcggccggcc ggctgcaccg cccagcacca
      121 gtgctgcctg acgtgtgacc gtcgtggaaa gctcgcaggt gatgctgatc aatctgtact
      181 ccgataccca gacccggcca gtcgacgaca tgcgcgcggc gatggcagcc gccgaagtag
      241 gcgacgagca gcgcggtgag gatcccaccg tcaacatgct gtgcgagcgg gtggccacgc
      301 tgctgggcaa gccggccgcg gtgttcctgc cctcgggcac gatgtgcaac gcgatcagtt
      361 tccggctgca catccggcct ggcggcgacg cggtacatct acatccgacg tctcacccga
      421 ttgtcgccga ggcgggcggc ccgtcggcgc tgtctgggac ggtgctcgcc ccggtccctg
      481 gtgaggccgg catgttcacc gctgaggccc tggaggcggc attgttcccc gccggggacc
      541 gctatgagcc acgctctcgg ctggtgtccg tggagcagac caccaacctg gccggcgggc
      601 gggtgtggcc gctggagcag atgcgagcag tgctggagat cgccgcgcgg caccggctgc
      661 gcgcgcacct ggatggggct cggctgatga acgccgtggt cgcctccggg gtgccggccg
      721 cggagtgggc gcgcgggttc gacaccgcct ggatcgactt caccaagggc ctgggtgcgc
      781 cggtgggtgc ggtgctggcc ggctcgactg agctgatcga ggaggcttgg cgctacaagc
      841 agatgatggg tggcgcgcta cgccaggccg gcatcgtcgc ggccggctgc ctgtgggccc
      901 tggaccacca cgtggaccgg ctcgccgacg atcacgccaa tgcccggatt ctcgccgcgg
      961 ggttggccgg gctacccggc atcacgctcg acccggacac tgtcgagacc aacatcgtgg
     1021 tgttcggtct ggcggatgct cctagattct gccagcggct ggaggccgag cacggggtgc
     1081 ggatgggcgc gctgggaccc gatgcggtcc gcgcggtgac ccaccacgac gtggacaccg
     1141 cagacatcca cagcgcagtg cacgcagtcg ccgcagtacg caaccctgca tgacgctgtc
     1201 ccctcggggc cgccattcgg aaccggattg cacttctggg cgcctcgctg cgtgccattc
     1261 ggagccgaat cacggtcaac gtcacggtgg ggcggtgagc gagcgcatca gggtggcagg
     1321 gacgatgagg tcggcgtggg atctggttga gctgatcagg acggcgttgc gttcgtcggt
     1381 gccggtcgcc caggcggccg ccacggcagg ttgcttgccg aaccggacat ggcgagcgat
     1441 gagccggcgc acacgctcgt ctgcgtcgag gtcgacgtac cagacctcgt cgagctgggc
     1501 ccggacgcga ccccagccgt gctcgtggag cagcaagtag ttgccttctg tgatgatcaa
     1561 tcgggtgcac ggcggtatcg ggatagcgcc ggcgatgggc tgttcgaggg agcgttggaa
     1621 gctcggggca tagaccacct cgtcgctgtc ttcgcgcagg cggcgcagca gcgccgcata
     1681 accggaagcg tcgaaggtgt cgggcgcg
//
LOCUS       DATADN010000040         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_758504, whole genome shotgun sequence.
ACCESSION   DATADN010000040 DATADN010000000
VERSION     DATADN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758504"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(496..759)
                     /locus_tag="VHH53_00510"
                     /pseudo
     CDS             complement(496..759)
                     /locus_tag="VHH53_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1010..2377
                     /locus_tag="VHH53_00515"
     CDS             1010..2377
                     /locus_tag="VHH53_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2288699.1"
                     /translation="MNVTGSQDHPLSPEVWEEAEMKRVLVDRDISSVYRLLRRVGISQ
                     RQIAALTGQSQSEVSEILKGRQVMAYDVLARISDGLGVPRGYMGLAYDDATATNLVAA
                     PDDALVEEDESVKRRKLLAHGAAVMFGTAVLGADSGKWLPSPSQTPAPSNIGMTDVKQ
                     VEAATRAMRALDYQFGGGACRDAVVAQLSWAQRLLSASSKEMVRTRLYRALGDLQNLA
                     GWTTFDVGLLDSSRSHFAAALEFAKQSGDSGLLSNIMYRIGRVYLHHDAPNDALKWFQ
                     LGQIAAQDSGSELAVAVLCGNEAWAYAMMGDDVQARKLLGRSRDELARADLSEAPDWA
                     RFYNETDMNAMIGTVHTVLSAIDVKHAAIAIPAINQSLTQYDESMARSRTFMLTALAT
                     NHLRQGDVDHGVRVGRDALTLATGLKSRRVADRLKPLEVEAGRRSSNSDSRQLSHLIR
                     QYRAV"
     gene            2389..3291
                     /locus_tag="VHH53_00520"
     CDS             2389..3291
                     /locus_tag="VHH53_00520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX2288700.1"
                     /translation="MTALSTLPRDQRLASGLLAICAELGLDPTGARLLRNVNNAVFRL
                     SRDPVVVRLVTLPSYIPRARLAVAAATVFAEHDVPAIRLLPGIPQPVSAGEHIATVWQ
                     ALPSVGPVPGGAQLAGLLRAVHAVPTPCRALPNWDPLTDFDNRMRHTTTIADADRDFL
                     LRRSAELATAVTELDFALPTAVLHGDAHLGNVIAGPAGPVLCDFDSCAIGPPEWDLTP
                     VAVANVRFGRPRAHQRDFIDAYGFDVRDWEGFEVLHGIRDLKLIAGVFPRAGSPPAVQ
                     AEFDRRMATLRAGRLSERWRTVRR"
     gene            3500..>4650
                     /locus_tag="VHH53_00525"
     CDS             3500..>4650
                     /locus_tag="VHH53_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tyrosine/L-tryptophan isonitrile synthase
                     family protein"
                     /protein_id="HEX2288701.1"
                     /translation="MGGGTTTVSISHARTLRALPLDLLRTSTLAVQQPVRLDDRELHT
                     LLTTLQANSEAASARLADNAVAHLATARGGWSPHHPPDVVAALHRILTRSRFLKGSRS
                     CFPLDTAMAQIAPLVEARQPVTIVTSGFPFKQHDNGLKAAGPWPDLAELGALLRLKEL
                     HRAFTALYPPGMHVLVLNDGGYSRPRESTEIRHYRRQLRRYAELIGLGHSVRFHDQSC
                     FVAGLLGERSWNKREQYRRTFRELISLLAAIPRSLDSAGQADRRLAEALPAMLLAGVP
                     SFRDILSSLVYSVPVPAPAGTDPRQWACEVLAWPDGYAEPDLSPDLASARREVVASAW
                     QNSVDYLAASLADAAAGVTGHYPPHVRLATVASRPGCSGFSYLGGSTLLP"
BASE COUNT          762 a         1531 c         1532 g          825 t
ORIGIN      
        1 ggggccgttc ctctcgaacg gccccgcccc attgcgggtg cctgcgctac acgcctccgg
       61 cgccggacgg gggaactccc gggcctgcgg tgactagtga ttcgaaccag gcgattcgag
      121 ctagcaccgg gcgatgggat ccggcgatca tcggatcaac gttcgcggtg gcggtcggtc
      181 aggcaacgac gatgccaccg gcgcggcact ccactcggtt gagagcgggg tgggtcaggc
      241 tgtggagttg tggaaaagct tgaggggtgt ggccggtgat ccttcgacat cgcggtcggg
      301 ggcacggacg ttcgccggat caccggccac ttggcggctg ctcaagccct cgtgggtggg
      361 gggaggccca caaaagcgac gaaaccgcca gctctatgaa gttgatctat taggagttga
      421 acggcattaa agccgctatc cgcctcccgc aggaagggga gaagccgttt gccaggtgtg
      481 aggcacggcc tcggccgaga cgaggtgttc gtgggcggcc cgccacacct tgcacggcga
      541 cgctcggggg cgccagtggc ttgagcactg tggacagcgg ccccgcgaat caggcgcatg
      601 ggtggcaagc agggcgcgcc agccgtaggt caaacgcggc agctcgctac gtgcaaccga
      661 cagcagtgaa ccgttatcgg cgctgcacgc cagctcgtcc agcatgtcca gccggtccca
      721 caccgcctgc cggaggatct gacccagcac ccggtccacc tcgccacctt ccctcgtcgc
      781 gcgtcgagaa tttccgccac accgggcggg ttctcgcaac gtcacaggta catagtcggg
      841 gtactaaacc cgttatgatc ctgtgttgcg ccgatcgggc atgctatgac gatgaacggc
      901 actaactgat cggtagcgcc gatcggcttt gccgaagtgc acttcaccaa cgctacgggc
      961 atgcctaggc tgcatgcgtc gcccgtcgga cggcttaata gggggcgaga tgaacgtgac
     1021 cggctcgcag gaccacccgc tgtctcccga ggtctgggag gaagcggaga tgaagcgggt
     1081 gctagttgat cgcgacatca gctccgtata ccggttgctc cgacgggtag gtatttcgca
     1141 acggcaaatc gcggccttga ccgggcagtc ccagtccgag gtctcggaga tcctcaaggg
     1201 ccgccaggtc atggcctacg acgtgcttgc gcgaatttct gacggtctag gtgtcccccg
     1261 cggatacatg ggcctcgcct acgacgacgc aactgcgacc aacctggtcg cagcgcccga
     1321 cgacgcgctg gtcgaggagg atgagtcggt gaagcgccgg aagctcctgg cgcatggagc
     1381 tgccgtgatg ttcggtaccg cggtgttggg ggccgacagt gggaagtggc tgcccagccc
     1441 ttcccagacg ccggcaccca gcaacatcgg tatgaccgat gtcaagcaag ttgaggcagc
     1501 taccagggcc atgcgcgccc tggactacca gttcggcggc ggcgcgtgcc gtgatgcggt
     1561 cgtcgcgcag ctgtcctggg cacaacgcct gctgtccgcg tcgagcaagg agatggttcg
     1621 cacccggctg taccgggcgc tgggcgatct gcagaacctg gccggatgga caaccttcga
     1681 cgtgggattg ctggactctt cgcgcagcca tttcgccgct gcgctggaat tcgccaagca
     1741 gtccggcgac tccgggctgc tgtccaacat catgtatcgg atcggccggg tgtacttgca
     1801 ccacgacgca ccgaatgacg ccctcaagtg gtttcagctg ggccagatcg ccgcgcagga
     1861 ctccggatcg gagctggctg tcgcggtgct gtgcggcaac gaagcctggg cctacgcgat
     1921 gatgggcgac gacgtgcagg ccaggaagct gctcggccga tctcgcgacg agctggccag
     1981 ggccgatctg tccgaggcgc ccgactgggc ccggttctac aacgagaccg acatgaacgc
     2041 gatgatcgga acggtccaca ccgtgctgtc ggccatcgac gtcaagcacg cggccatcgc
     2101 catccccgcg atcaaccaat ccctcaccca atacgacgag tcgatggctc gtagccggac
     2161 cttcatgctc accgcactgg ccaccaacca cctgcgccaa ggcgacgtcg accacggtgt
     2221 ccgggtcggt cgagacgccc tgaccctggc cactgggctg aagtccaggc gagtggctga
     2281 ccggttgaaa cctttggagg tcgaggcggg gcggcggtcc agtaactccg attcccgtca
     2341 actctcacat ctgatccggc agtaccgcgc tgtatgaggc aagctgtcat gacggcgctg
     2401 tcgactcttc cacgtgatca aagactcgcc agcggactgc tggcgatatg cgccgaactc
     2461 ggtctggatc caaccggggc aagattgctg cgcaacgtga acaacgcagt tttccggctg
     2521 tcccgagatc ctgtggtggt ccggttggtc accctgccct cctacatccc acgggccagg
     2581 ctggcggttg ccgccgcgac ggtattcgcc gagcatgacg tgccggcgat tcgattgctg
     2641 cctggcatcc cgcagccggt gtcggccggc gaacacatcg ccaccgtgtg gcaggcgctg
     2701 ccatcggtcg gccccgtgcc cggcggtgcg caactggccg gcctgctgcg agccgtgcat
     2761 gcggtaccga cgccgtgccg agcgttgccg aactgggatc cactcactga tttcgataat
     2821 cggatgcggc acaccaccac gatcgctgat gccgaccgcg acttcctcct gcgacgcagt
     2881 gccgaactgg ccaccgccgt cacggagctg gattttgcgc tgccaacggc cgtgctgcac
     2941 ggcgatgccc atctgggcaa cgtcatcgct gggccggctg gtccggtgct gtgtgatttc
     3001 gattcttgcg ccatagggcc gccggagtgg gatctgaccc cggtcgcagt agcgaacgtc
     3061 cggttcggca gaccccgtgc ccaccagcgt gacttcatcg acgcctacgg gttcgacgtc
     3121 cgggactggg aaggcttcga ggtgctgcac ggtatccgcg acctcaagtt gatcgccggg
     3181 gtattcccgc gggccggttc cccgccggcc gtgcaggcgg agttcgaccg ccggatggct
     3241 accctgcggg ctggacggtt gtccgaacgc tggcggaccg tccggcgctg agccggcttc
     3301 gaccactgaa ccgcttcgac cactgagccg cgtcaaatac tgagctgtcc aattactgga
     3361 ctgctcaact accggaccgt gcacgtgcaa ctgggggatg cgtcaaggca cccggacgtc
     3421 tactgtcgag ccggtgtcat tggctcccct atcgcgggcg ggagtgctgg tcccgcctcg
     3481 gactgagagc ggcgatgcag tgggcggggg caccacgaca gtcagcattt cgcatgcccg
     3541 gacactacgg gcgcttccac tggacctgct gcgaacctcg accctggccg tacagcagcc
     3601 ggttcggctg gatgacaggg agctccatac gctgctgacc actctccagg ccaactcgga
     3661 ggctgcctcg gcccgcctcg ccgacaatgc cgtggcgcat ctggccactg cgcggggagg
     3721 ctggtcacca caccacccgc cggatgtggt ggcggcgcta caccggattc tgactcgttc
     3781 gcggttcctc aagggttcac ggtcctgttt tccccttgac accgcgatgg cacagatcgc
     3841 gccgttggtc gaagcccggc agccggtcac gattgtgacc agcggttttc ctttcaagca
     3901 gcacgacaat gggctcaagg cggctggtcc ttggccggac ctggccgagc ttggtgcgct
     3961 gctgcgactc aaggagctgc accgggcctt caccgcgctg tacccccctg gcatgcacgt
     4021 gctggtgctc aatgacggtg gctactcacg accgcgcgag tcgacagaga tccggcacta
     4081 ccggcgccag ctacggcgct acgcggagct gatcgggctc ggccactcgg tgcgcttcca
     4141 cgaccagtca tgcttcgtcg ccggcctgct cggcgagcgg agttggaaca aacgtgagca
     4201 gtaccgccgc acgtttcgcg agttgatctc gctgctggcc gccattcctc gctcgctgga
     4261 cagcgccggc caggccgatc gacggctggc cgaagcgctg cctgccatgt tgctggccgg
     4321 tgtgccgtcc ttccgtgaca ttctctcctc gctggtgtac tcggtgccgg tacccgctcc
     4381 agcggggact gacccacggc aatgggcctg tgaggtgctg gcgtggccgg acgggtatgc
     4441 cgagccggat ctgtcgccgg acctggcctc agcccggcgg gaggtcgtcg cgtcggcgtg
     4501 gcagaactcc gtggattacc tcgcggcttc cctcgctgac gccgccgccg gcgtcaccgg
     4561 gcattacccg ccacacgtcc ggctggccac ggtggcctcc cgaccgggct gcagtggttt
     4621 ctcctacctc ggtggctcaa cgctgttgcc
//
LOCUS       DATADN010000041         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_758675, whole genome shotgun sequence.
ACCESSION   DATADN010000041 DATADN010000000
VERSION     DATADN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758675"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VHH53_00530"
     CDS             complement(<1..445)
                     /locus_tag="VHH53_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2288702.1"
                     /translation="MAGKSGISQARRRLGEAPLRRLYEELAQPIATRASKGAWYRDWR
                     LVSLDGSCLEVADTEANRTEFGLPGASRGESAFPQLRFVALVENGTHVLFGARLGPYA
                     EGETTLAHKVLAALRPGMLCLADRQFFGHALWLRARDTGAYLLWRV"
     gene            complement(504..1625)
                     /locus_tag="VHH53_00535"
     CDS             complement(504..1625)
                     /locus_tag="VHH53_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX2288703.1"
                     /translation="MNVRYRVDLSQAERDQLSALLSGGTHASRKLKRAQILLAADAGV
                     SDNDIAASIAVGGSTVYRTKRRFVEGDLEAALSEEQRLGAARKLTGKEEALLVATACS
                     NPPEGRARWTLELLAGAMVRLTEHEELSRETVRRRLAENHLKPWRKDMWCIPQIDGTY
                     VARMEDVLDLYAEPPDPKRPVVCFDESPTQLIGEARQPIPAAPGQLERYDYEYRRNGT
                     VNLFVFLDAHRPWRRVKVTDQRTARDFAECMRELVDLHYPQAQQVRVVLDNLSTHSAG
                     ALYEAFPAPEAHRVLRRLEFHYTPKHASWLNMVEIEIGVLRGQCLDRRIGARDCLVSE
                     IAAWEQQRNAAGSRITWMFTTERARTKMARAYPDPAKVS"
     gene            complement(1910..2197)
                     /locus_tag="VHH53_00540"
     CDS             complement(1910..2197)
                     /locus_tag="VHH53_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEX2288704.1"
                     /translation="MAGVAAGLPAGIRLSDHISLGVIARAVPPERVRQVLAETGKASE
                     RERDLPAQVMVYYAIALALYMGSSTREVLRCLLVPLHRRIDGLIVTGAEGR"
BASE COUNT          398 a          796 c          753 g          426 t
ORIGIN      
        1 tcacccgcca gagcaggtag gcgccggtgt ccctcgccct cagccagagc gcatgaccaa
       61 aaaactgccg gtcggccaga cacagcatgc cgggacgcag cgccgccagc accttatggg
      121 ccagcgtcgt ctcaccctcc gcatagggcc cgagacgggc gccgaacagc acgtgcgtgc
      181 cattctccac cagcgccacg aaacgcagtt gcgggaaggc gctctcgcca cggctggccc
      241 cgggtaggcc gaactcggtc cggttcgcct cggtgtcggc cacctccagg caggagccgt
      301 ccaggctgac cagccgccag tcgcgatacc acgccccttt gctggcacgc gtggcaatcg
      361 gctgcgccag ctcctcgtac agccgacgca gcggtgcttc cccgagccgg cggcgtgcct
      421 gcgaaatccc gctcttgccc gccaccctga ccgcctcggt gccccacagc cagcgcagac
      481 cctctagtac ctctgcacag ggattatgac actttggcag ggtcggggta agcgcgggcc
      541 attttggtgc gagcgcgctc ggtggtgaac atccaggtga tgcgggagcc ggcggcgttg
      601 cgctgctgtt cccaggcggc aatttcggag acgaggcagt cgcgcgcgcc gatgcggcgg
      661 tcgaggcact ggccgcgcag cacgccgatc tcgatctcga ccatgttgag ccagctggca
      721 tgctttgggg tgtagtggaa ctccaggcgg cgcaatacgc ggtgcgcctc gggtgccggg
      781 aaggcttcat aaagcgcgcc ggcagaatgt gtcgatagat tgtccagcac gacccggacc
      841 tgctgggctt gcggatagtg gaggtcgacc aactcgcgca tgcactcagc aaagtcgcga
      901 gctgtccgct ggtcggtaac cttcacacgg cgccagggcc ggtgggcgtc gagaaagacg
      961 aacaggttga ccgtgccgtt gcggcgatac tcataatcat agcgttccag ctggcccggc
     1021 gcggcgggga tcggctgtcg cgcctcgccg atcagctggg tcgggctctc gtcgaagcat
     1081 accaccggcc gctttggatc ggggggctcg gcgtacaggt cgagaacatc ctccatgcgg
     1141 gcgacgtagg taccgtcgat ctgcggaatg caccacatgt ccttgcgcca cggcttgagg
     1201 tggttctcgg ccaagcgccg gcgcaccgtc tcgcgtgaca gttcctcgtg ctcggtaagc
     1261 ctgaccattg cgccggccaa caactccagc gtccaacgcg cccggccctc gggcgggttc
     1321 gagcaggcgg tcgccaccag cagggcttct tctttgccgg tgagtttgcg cgccgcgccg
     1381 agccgctgct cttcgctgag cgcggcttcc agatcgcctt ccacaaagcg gcgcttggtc
     1441 cggtacaccg tcgacccgcc gaccgcgatg ctggcggcga tatcgttatc actgacgcca
     1501 gcatcggcgg ctagtaggat ctgggctcgt ttgagcttgc gcgaggcgtg cgtgccgccg
     1561 ctcagcaagg cgctcaactg gtcgcgctcg gcttggctca ggtcgacccg gtaacgtaca
     1621 ttcatcgctg tcccctcgat tgagggcaca acagatgaat cacatgcctg aaggatgtcg
     1681 ccccgcttgc gcgccgcgct ttacgcccaa gcaggggcag tatttagcct tcatccatgc
     1741 ctatacgctg gtgatcggcc gaccgccggc cgaagccgat ttgcaacgat tcttccaggt
     1801 gacgccgccg tcagtccatc aaatggtgat cacgctggaa cgcaacgggc tgatccgccg
     1861 ccgccccgga acggcccgaa gcatcgaggt gctggtcgat cccggagcgc taccggccct
     1921 ccgcgccagt cacgatcaac ccgtcaattc ttctgtgcag aggtactagc aggcagcgca
     1981 gcacctcgcg cgtgctcgag cccatgtaga gcgccagagc aatggcgtag tagaccatca
     2041 cctgcgccgg caggtcgcgt tcgcgctcgc tcgccttgcc ggtttccgcc agcacctggc
     2101 gcacccgttc gggcggtact gctcgggcga tcaccccaag gctgatgtga tcgctgaggc
     2161 ggatgccagc tggaaggccg gcagcaacac cggccatcgc taccgatccc atatcatcag
     2221 gccggcagcc tacgatccta atcagcctaa gtgaacggta ttggggcaag gccggattac
     2281 actctcggtg ctcgatctat tttattttgt cttattctat ttttccttgt cgcggcccgg
     2341 cgcgcggcga tggcgcaacg gatcgggtct ggc
//
LOCUS       DATADN010000042         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_4837, whole genome shotgun sequence.
ACCESSION   DATADN010000042 DATADN010000000
VERSION     DATADN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4837"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1193
                     /locus_tag="VHH53_00545"
     CDS             90..1193
                     /locus_tag="VHH53_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX2288705.1"
                     /translation="MQRYRGSQLFRAGFIGVALIILIIAVGLQPERIISWATSLRYQA
                     MFTEAGGLTVGNDVTVSGIKVGSVSSIALDNGDALVGFTIDGRYALGSDTTAHIRTGT
                     LLGERVLALESEGSGTLDRSQVIPVTRTSSPYSLTDAISELTSNTAGTDTQQLNQSLD
                     TLSQTLDQVAPQLGPTFDGLSRLSESLNNRNDSLAELLRTAGDVTQIFSERSNQVNTL
                     LLNANDLLAVLNERRYAITGLLQNTAAVSQELTTLVANNEQELAPALRHLNEVTAMLQ
                     KNRDNLAKMLPGAAKYYLTQGEIVSNGAYYNALVPNLQPAQLLQPFLDYAFGFRRGMD
                     AGQPPDNAGPRAELPFPVNSIPQPGDLPDDGNP"
     gene            1180..>1971
                     /locus_tag="VHH53_00550"
     CDS             1180..>1971
                     /locus_tag="VHH53_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX2288706.1"
                     /translation="MATRKPLVVGTAVLLAIALIAGAAYLVRQMFFGPNTITAYFPTA
                     TAIYPGDEVRVSGVEVGTIESITPAGTQTKMTLKVDRGVPVPADAKAIIVAQNLVAAR
                     YVQLTPAYRKGDGPTMKDGAVIPAERTAVPVEWDEVKTQLMRLATELGPETGVSDTSV
                     SRFIDSAANAMGGNGDKLRQTLAQLSGAARIFAEGSGNIVDIIKNLQIFVSALRDSKQ
                     QIVMFENRLATLSSVVNDSRSDLDAALSNLSVAMGEVQRFVAGSRD"
BASE COUNT          379 a          648 c          624 g          320 t
ORIGIN      
        1 ggaatcagct tccgcgccag cgatctacag ggccaaacgg tgatattccc gtggatcaag
       61 caggaaacgg gaaggtgcgt ggacagctga tgcagagata ccgcggttcc caactcttca
      121 gggcgggctt catcggcgtc gctttgatca ttctgatcat cgccgtcggc ctgcagccgg
      181 aacgcataat ctcgtgggcc acctccctgc gctaccaggc gatgttcacc gaggccggcg
      241 gactgacggt cggcaacgac gtgacggtgt ctggcatcaa ggtcggctcg gtgtcctcga
      301 tcgcactcga caacggcgat gcattggtcg gcttcaccat cgacggtcgg tatgccctcg
      361 gttccgacac caccgcgcac atccgcaccg ggacgctgct cggcgagcgg gtgctggcac
      421 tggaatcgga gggcagcggg acgctcgacc gcagccaggt gattccggtg acgcgcacgt
      481 cgtcgccgta ctccctgacc gacgcgatca gcgagctgac gtcgaacacc gcaggcaccg
      541 acacccaaca gctcaaccag tcgctggaca cgctgtcgca gacactcgat caggttgcgc
      601 cgcaattggg cccgaccttt gacggcctct cgcggctgtc ggagtcgctg aacaatcgca
      661 acgacagcct ggccgagctg ctgcggaccg cgggcgacgt cacgcagatt ttctccgagc
      721 gcagcaacca ggtgaacacg ctgctcctca atgccaacga ccttctcgcg gtgctgaacg
      781 agcggcgcta cgcgatcacc ggactgctgc agaacaccgc ggcggtgtcg caggagctga
      841 ccaccctggt cgccaacaac gagcaggagt tggcgccggc actcaggcac ctcaacgagg
      901 tcaccgcgat gctgcagaag aaccgcgaca acctggcgaa gatgctgccc ggagccgcga
      961 aatactatct gacccagggt gagatcgtct cgaacggcgc ctactacaac gcgctcgttc
     1021 cgaatctcca gccagcccag ctgctgcagc cgttcctcga ttacgcgttc ggattccggc
     1081 gcggtatgga cgccggacag ccgccggaca acgccggtcc gcgggccgag cttccgttcc
     1141 ccgtcaacag cattccgcag ccaggagacc ttcccgatga tggcaacccg taagccactg
     1201 gtggtcggga cagcggtcct cctggccatc gcactgatcg ccggcgcggc atacctcgtg
     1261 cggcagatgt tcttcgggcc caacaccatc acggcctact tcccgaccgc gaccgccatc
     1321 tatcccggcg atgaggtgcg ggtgtctggc gtagaggtcg gcacgatcga atcgatcacg
     1381 ccggcgggca cccagacgaa gatgaccctc aaggtcgacc gcggagtgcc ggttccggcg
     1441 gacgccaagg cgatcatcgt cgcgcagaat ctcgttgcgg cgcgttatgt tcagctcaca
     1501 ccggcctacc gcaagggtga cgggccgacg atgaaagacg gcgccgtcat tcccgccgag
     1561 cgcaccgcgg tcccggtcga gtgggatgag gtcaagaccc agttgatgcg actggcaaca
     1621 gaactgggac cggagaccgg ggtttcggac acgtcggtgt cccggttcat cgacagcgcg
     1681 gcgaacgcga tgggcggcaa cggcgacaag cttcggcaga cgctggccca attgtccggt
     1741 gctgcgcgga ttttcgccga aggcagcggc aacatcgtcg acatcatcaa gaacctgcag
     1801 atcttcgtgt cggcgctgcg cgacagcaag cagcagatcg tcatgttcga gaaccggctc
     1861 gccacgctga gcagtgtggt caacgacagc aggtccgacc tggacgctgc gctgtcgaat
     1921 ctctctgtgg cgatgggcga ggtgcagcgg ttcgtcgcgg gcagccgcga c
//
LOCUS       DATADN010000043         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_306538, whole genome shotgun sequence.
ACCESSION   DATADN010000043 DATADN010000000
VERSION     DATADN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306538"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..1084
                     /locus_tag="VHH53_00555"
     CDS             368..1084
                     /locus_tag="VHH53_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2288707.1"
                     /translation="MAAVGYGATVRSAPSGAVPASLVPHPREELFTVLVVDDHPLLRE
                     AIASRLVTMGAVTVHEAASVAEARTRALASGPCDLAILDLTLPDGSGLDLIAELRGQG
                     WPRVVVLASSDDPHAVRTAFQTGAQAYLLKSASPSVVTDGVRRVLDGGVYADPTVAPM
                     LASGTRVPGTDNTPRELSAREVEVLQLVADGQSNKEIGEALKLSALTVKSHLSRIGRK
                     LGTGDRAQMVALAMRAGVIR"
     gene            1081..>2127
                     /locus_tag="VHH53_00560"
     CDS             1081..>2127
                     /locus_tag="VHH53_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX2288708.1"
                     /translation="MTETTPVPLLAPAAGVPPVVASAAALDAAVAVLASGAGPVAVDT
                     ERASGYRYWQRAYLIQLRREGAGTTLIDPIGLDDLAGLADVLNSLEWVLHAASQDLPC
                     LAELGLRPYAIFDTELAARLAGLERVGLAALVEELLGYHLQKGHGAADWSRRPLPAEW
                     LTYAALDVELLLPLRAVIAAELDAQGKSGWAAEEFEAVRTAAPPAPRPDPWRRTSGMH
                     RVHRPRELAAVRELWLAREQLARQRDIAPGRVLPDAAIIDAALADPHDDAALLSRPVF
                     GGRSQRRQVKTWLTALQTARTLPAEQLPPVHAHNGSPPPVNRWPDRDPAAAVRLVAAR
                     AALAEIAEHHRLPLQ"
BASE COUNT          307 a          741 c          740 g          339 t
ORIGIN      
        1 tctgtgaact gatggcggca gccgccgggc tcccaccggt tggagttgtg gccctcggca
       61 agcgcctcga ctgatcaagg ctgcgacccg actcccgcaa cgacctgcga tttacgcatt
      121 aacttaaagg agtcgtccga ctcgacgctg gcgttactcg tccggcgctg tatgccctga
      181 ttgggcgacc gtcgatcgcg gctttgcgat caaatcggca agaaggttcc ctcgcagctc
      241 cccgttaggc cgctcggacg gctagtctcc cccgtacgac accatccccg gtcgagtggc
      301 ggcgcggtta ctcgacgggg tcccggtgtg cggtcggggg cacgaccgac tccagggagg
      361 tagcgacgtg gctgctgtcg gatacggcgc gaccgtcagg tcggcgccat ccggcgccgt
      421 gccggcgtca ttggtcccgc atccgcggga ggaactgttc accgtgctgg tggtcgacga
      481 ccatccgtta ctacgtgaag cgattgcgtc acgcttagta acgatgggcg cggtcactgt
      541 gcatgaggcc gcatcggtgg ctgaggcgag gacacgtgct ctcgcctcgg gtccgtgcga
      601 cctcgcgata ctcgatctga ccctgcccga cggtagcggg ctggacctga tcgccgagct
      661 gcgcggccaa ggctggccgc gcgtcgtcgt gctcgcatca tcggacgacc cgcatgcggt
      721 ccgcaccgca ttccagaccg gtgcgcaggc gtacctactc aagtcggcgt ccccttcagt
      781 ggtcaccgat ggcgtgcgcc gagtgctcga tggcggggtg tacgcagacc cgacggtggc
      841 gccgatgctg gcgtccggca cgcgggtacc gggaaccgac aacaccccgc gcgagctgtc
      901 cgcccgggag gtcgaggtgc tccaactggt cgccgacggc cagtccaaca aggagatcgg
      961 tgaggccctg aaactgtccg cgctcacggt gaaaagtcac ctgtcccgga tcgggcgcaa
     1021 gctcggcacc ggcgaccgcg cgcagatggt ggctctagcg atgcgtgcgg gggtcatccg
     1081 ctgaccgaga ccaccccggt tcccctgctg gcgccggccg ccggggttcc gccggtcgtc
     1141 gcctctgcgg cggctttgga cgctgcggtg gccgtactcg cttccggcgc cggcccggtc
     1201 gctgtggaca ccgaacgcgc atcgggttat cgctactggc agcgcgccta cctgatccag
     1261 ctccggcgag agggcgcggg caccacactc atcgacccga tcggcctcga cgacctggcc
     1321 gggctcgccg acgtgctgaa ctcactggaa tgggtgctgc acgccgcgtc ccaggacctg
     1381 ccgtgcctgg ccgagctggg gctacggccc tacgcgatct tcgataccga gctggccgcg
     1441 cggctggccg gactcgagcg ggtgggcctg gcggcgctgg tcgaagaact gctcggatat
     1501 cacctgcaga agggacacgg cgccgctgac tggtcgcgcc ggccgctgcc ggcggagtgg
     1561 ctgacatacg ctgcgctcga cgtcgaactg ctactgccgc tgcgagctgt gatcgccgcc
     1621 gaattggacg cccagggcaa gtccggttgg gctgccgagg agttcgaggc ggtccggacc
     1681 gcggctccac ccgcaccgcg tcccgacccg tggcgccgga cctcgggaat gcaccgcgtc
     1741 catcggccac gcgagctggc cgcggtgcgt gaactgtggc tggcccgcga gcagctggcc
     1801 cggcagcgtg acatcgctcc cggccgggtg ctgcccgatg ccgcgatcat cgatgcggcg
     1861 ctcgccgatc cgcacgacga tgcggcgctg ctgtcccgcc cggtgttcgg cggccgttcg
     1921 cagcggcggc aggtcaagac atggctgacc gcgttacaaa ccgccaggac gttgccggcc
     1981 gaacagctac caccagtgca tgcccacaac ggcagcccac ctccggtcaa ccgctggccg
     2041 gaccgggatc cggccgcagc ggtccggctt gtcgcggcgc gcgcggcgct ggcggagatc
     2101 gccgagcacc accggctgcc gttgcag
//
LOCUS       DATADN010000044         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1361884, whole genome shotgun sequence.
ACCESSION   DATADN010000044 DATADN010000000
VERSION     DATADN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361884"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..166
                     /locus_tag="VHH53_00565"
     tRNA            90..166
                     /locus_tag="VHH53_00565"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:124..126,aa:Val,seq:tac)
     gene            362..733
                     /locus_tag="VHH53_00570"
     CDS             362..733
                     /locus_tag="VHH53_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2288709.1"
                     /translation="MRLRLELFVDDLDRSVDFYRRVLGFHLVRQDPGYASLCNGAVVL
                     GLGPAAGLPEGRGAGVEVVLEVDDLEAAYERVRRAGVRQVEAPRERPWGLRDFRILDP
                     DGYYLRVTDGEAGCDPDKERQ"
     gene            1253..1558
                     /locus_tag="VHH53_00575"
     CDS             1253..1558
                     /locus_tag="VHH53_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288710.1"
                     /translation="MDNQASRRLPTQPNEPAGQPPEDGEPDYVLWIYLGAESVFFYPP
                     GSVQEHLVIEHGIPSVDMHWKVEGYYSHEGDHHRQQGVRSGAVGDLLTVTHGHEQAA"
     gene            complement(2112..>2732)
                     /locus_tag="VHH53_00580"
     CDS             complement(2112..>2732)
                     /locus_tag="VHH53_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2288711.1"
                     /translation="DHRDQHGGRRAGGWHASVGSGSLMIVMLLLLYPMLRSSELVGTD
                     LVQAIPLVASAAAGHLLFGDFQLDLAASLLVGSLPGVYLGARLSSRANDAVIRPALAL
                     VLVASGLKLLGVDNLQLGLLVLVLVLLAVPVWGALDATLHPEPAWAGAPVGRRMTVGV
                     QLIGAPLGLGFVAALAYFGLLRPRLIAAVQVAPALAEPQAPTRFGR"
BASE COUNT          507 a          960 c          930 g          335 t
ORIGIN      
        1 ggttccgcat gaggaggccc cagagcacct cgcttcgctc ccataggact ggacagaaac
       61 acagtcccac gagcaagaac acaccaccag ggcgtgtagc tcagctggcc agagcgcgtc
      121 gcttacaacg acgaggtcac aggttcgagc cctgtcacgc ccaccagccg aaccggcagg
      181 ccaacgcgcc ggatcaccgc cctgaccagg ccgcgcagcc actcaacccc ctccagggcc
      241 gcgctggtgg ccgaagcatg tcccaacccg gccgggtttg ccgtggcgtc ggtgacacta
      301 ggatgggccc atgtcaccca cctcgtcccg ctgctcggca gcaacgctgg acgcctgacc
      361 gatgcggctg cggctggagc tgttcgtcga cgacctggat cgcagcgtcg acttctaccg
      421 tcgcgtgctc gggtttcacc tcgtccgcca ggacccgggc tatgccagcc tgtgcaacgg
      481 cgcggtggtg ctcggccttg gcccggccgc cggcctgccc gagggccgag gtgccggcgt
      541 cgaggtcgtc ttggaggtcg acgacctgga ggcggcctac gagcgggtca ggcgggccgg
      601 cgtgcgccag gtcgaggcgc cgcgcgagcg gccgtggggg ctgcgggact tccggatcct
      661 cgaccccgac ggctactacc tgcgtgtcac cgacggtgag gccgggtgcg acccggacaa
      721 ggagcgtcaa tagggccgga gtgggcatcg gcttctgagc tcggcacgta gagccgcccg
      781 aaactgcatg gcatgcacga ggtcagagcc gcgatggtcc ggcgtgtacc gaacatcacg
      841 tgccagccgt gaccgtcggc acagcgctgg ccgagccgac ccatcatcct gaggcggttc
      901 agaaccgtgg tgtgcgcatc acccgccgag cagcacgctc ctcgaaagga ggaaccgcct
      961 gatgacgctc tgggaccacc taggcgggcg gacctggagc cagcgcgccg gcccgcgacc
     1021 agccgatgca gagggagctc gtcgccgccc gagccgacgt caccagctca cacggtcctc
     1081 tctcaaggca cgcaaacccc gccacagcca gtaagagacg caaggccgag atcgccgtag
     1141 gtcaggcgtc gcccgacaac caggatcgcg gtcgacagca gccgagaacc tggtcggggc
     1201 gttgggggcg ccggcagcga caagaccgga caccgaagca aggtttgagt ccatggacaa
     1261 ccaagcgagc cgacgactgc ccactcagcc caacgaacct gccggccagc cacccgagga
     1321 tggcgagccg gactatgtgc tgtggatcta cttgggcgcc gagtcggtct tcttctaccc
     1381 gccgggatcg gtccaagagc atctggtcat cgagcacggc atcccttcgg tcgacatgca
     1441 ctggaaggtc gagggctact acagccacga gggcgaccat cacagacaac agggcgtgcg
     1501 atcgggcgcg gtcggcgatc tgctcaccgt cacccatggc cacgagcagg ccgcatgacc
     1561 ttgggcacag ctggggcgta gcgaccaggc ccgcgcgaca accacggtca ccggcggacg
     1621 acaacacccg aggtcgacca ggctgccccc aggtagctag gcccgagcct gcgctgggca
     1681 acggcgacca tgaccagggc gacgcggagg cagagccagt agccggttcg gccacgacga
     1741 cgggacgggg cgaaggcgaa gcatgctagc gttccgttcc gtcatgtctg tgctctggag
     1801 cttcgttgtg agcagttctc tacgaactca aggccggaca gcgggcggcc gcaggccgtc
     1861 ctcccagccg gcagcgatcc gacggccacc ggggtgccgg cctcaccctc gaaccagggg
     1921 agccgaagcc aggacggggg tgcttcctgg gctgtgctgg ggccgcagcc gggctgtgaa
     1981 tgaccggagc gcctcggaca accaggggca gccgcgggca tcatctgtcc agctgagcag
     2041 ccctattcgg ccgagcgcac caggccagag ctagccgccg atcgctctcg cacggcggaa
     2101 gtccctggag gtcagcgccc gaaccgggtt ggggcctgcg gctcggccag cgcgggcgca
     2161 acctggactg ccgcgatcag ccggggtcgc agcagcccga agtaggccag cgcggccaca
     2221 aagccaagtc cgaggggcgc accgatcagc tgtacgccga ccgtcatgcg tcggcccacc
     2281 ggggcgccag cccaggccgg ctccggatgc agggtcgcat ccaatgcccc ccacaccggc
     2341 acggccagca ggaccaggac aagcaccagc aggcccagct ggaggttgtc gaccccgagc
     2401 agcttcagcc ccgaggcgac caggaccagg gccagggccg gccgaatcac cgcgtcattg
     2461 gcccgcgagg acagcctggc gcccaggtac acgcccggga ggctgccgac cagcagcgac
     2521 gccgccagat ccagctggaa gtcgccgaac agcaggtgcc cggcggcggc cgaggcgacc
     2581 agggggatcg cctggaccag gtcggtgccg accagctccg aggagcgcag catcgggtac
     2641 agcaacaaca gcatgacgat catcaacgag cccgaaccga ccgaggcatg ccaaccacca
     2701 gcccgccgac caccgtgctg atcgcggtgg tc
//
LOCUS       DATADN010000045         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1362018, whole genome shotgun sequence.
ACCESSION   DATADN010000045 DATADN010000000
VERSION     DATADN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362018"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VHH53_00585"
     CDS             complement(<1..845)
                     /locus_tag="VHH53_00585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2288712.1"
                     /translation="MRDSLRDILWAQRLRRTMRTKLQRAIQEVVGPPHAEQMRELASL
                     RQEVSELRSRLIEDMGELVRSTAERVIDQTRALEIRSRRDICYAGDVQAARESAEFAR
                     GHLAGARQFGHPHATLKYALSLAPVGGMALEFGVYTGTTLKIIATARQGGRVYGFDSF
                     EGLPEHWRAGFAAGTFAADTVPDVPGAELVVGCFDEVLPEFMRNYPGPVDFLHIDADL
                     YSSAATVLNLVGPRLREGSVVVFDEFFNYPGWQEHEYRAWTEYVERTGIAFRYEAFTF
                     DNEQVV"
     gene            complement(1209..1466)
                     /locus_tag="VHH53_00590"
     CDS             complement(1209..1466)
                     /locus_tag="VHH53_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288713.1"
                     /translation="MDADEALEALLGDQRTGDLRTDLIEGLKEAMRRRERNAEHGGVV
                     IAALVDMGLSYRDIEHVTELPRTTAQRWAIPPERKRIAEEQ"
     gene            2064..3908
                     /locus_tag="VHH53_00595"
     CDS             2064..3908
                     /locus_tag="VHH53_00595"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2288714.1"
                     /translation="MSEHEQWRELAQQFRVDSVRASGTAQSGHPTSSMSAADLMAVLV
                     TKYLHYDFGDEDNPNNDHLIFSKGHASPLLYAIHKAAGVVSDEELLTYRTLGSRLEGH
                     PTPTAPGVDVATGSLGQGLPVGVGLALSGKRLDRLPYRVWVLCGDSEMTEGSMWEALE
                     HAGHAGLDNLTAIIDVNRFGQRGTTMHQWNLDAFARPAEAFGWHVITVDGHDVGAIDE
                     AYAEAVETKGQPTVIIAKTLKGKGVSEVEDTELHGKPVKDADKAIEELGGIRNIQIAI
                     PTRTSDAKPPSFEVSSAERPRYELGEEVATRKAYGEALAALASCRGDVVALDGEISNS
                     THADIFAKAHPERFFEMYIAEQLMVAVAVGFQVRGWVPYASTFAAFLSRAYDFVRMAA
                     ISRASLNLVGSHAGVSIGEDGPSQMGLEDLAMFRAVHGSTVLHPCDANQTAQLVWQMA
                     DRPGIRYLRTLRPSTPVIYGPDEHFEIGGSQVIRHSDGDQVALIAAGITVHEALEAAE
                     ALEAEGVRARVIDCYSIKPIDAETLREAARATGGRILTVEDHWPEGGLGDAVLEVFAQ
                     AEECPRIVKLAVRKMPGSGPSEKLLKDVGIDARAIAEEARKLAKATPR"
     gene            4015..>4918
                     /locus_tag="VHH53_00600"
     CDS             4015..>4918
                     /locus_tag="VHH53_00600"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX2288715.1"
                     /translation="MTRGVVAAGHPLTAEAGASALRAGGNAVDAALAAMCASFACEPL
                     LTGLGAGGYMLVGAPGGDDVLLDFFVEAPGRGGDPPRRAELVPVSVLFGDVVQVFHVG
                     AASVGTYGCPAGICAAHARFASLPLAKLVAPAVRLAREGVALNARQTHIIELLWPIIT
                     SSPQARALFAKAPPREGEVLRLPDVADGLERLAAEGAAPFYTGELATAVCDWIGERGG
                     MLSPADLAAYKVVPREPIEVPYRGLRVRTNPPPSAGGILIAAVLAELDREPAPPSRER
                     LVAAMIDAQGARTAAFMESLHIRGF"
BASE COUNT          798 a         1665 c         1603 g          852 t
ORIGIN      
        1 acgacttgct cgttgtcgaa agtgaacgcc tcataccgga acgctattcc ggtgcgttcc
       61 acgtattcgg tccatgcccg gtattcgtgc tcctgccagc cgggatagtt gaaaaactcg
      121 tcgaacacga caacgcttcc ctcccggagg cgcgggccga ccaggttaag cacggtggcg
      181 gctgaagagt acaggtcagc atcgatgtgc aggaagtcga cggggccggg atagttcctc
      241 atgaattcag gtaaaacttc gtcgaagcac cccaccacca actcggcccc cggaacgtcg
      301 ggcaccgtgt cggcggcgaa cgtcccggcg gcgaagccgg cccgccagtg ctctgggagg
      361 ccctcgaagg agtcgaatcc gtagacccgc ccgccctgcc gtgccgtggc gatgattttg
      421 agcgtggtgc cggtgtagac gccgaattcc agtgccatac cgcctacggg agcgagagac
      481 agcgcgtatt tcaacgtggc gtggggatga ccgaattggc gcgcgccggc taggtggccc
      541 cgggcgaact cggcgctttc tcgggccgct tggacgtcac cggcgtagca gatgtctcgt
      601 cggctgcgga tttccaaggc cctcgtttgg tcgatgaccc gttcggcggt cgaccggacc
      661 aactcgccca tgtcttcgat cagtcggctc cgcagctcgc tcacttcctg ccgcaggctt
      721 gccagctcgc gcatctgctc ggcgtgaggg ggtccgacca cctcctgaat ggcccgttgg
      781 agcttggtcc gcatcgtcct gcgcaaccgt tgggcccaga ggatgtcgcg caggctatcc
      841 ctcatcccct caactcgtct ctgacgacac cgtggtctct cacgccgtgg ccctcctgca
      901 gtggtttccc tacaccgcga agtcaccatt cacacatccg acagaggcca ccggcccagc
      961 caggtgtgac cgacaccccg ctgtccccgg ctcagcacgg gtgctcagtg caggacatgc
     1021 attcgttcaa aactcgcgcc gcggtcacta cgaaactggc tgttggcctc cgcgctaacc
     1081 accgaatctc agttagtttt caccgaacgc gcactcacca tctaacaaca cccgcaccgc
     1141 aatcgacggc gcctgtcatt ccgataacgc aacagcgccc gcgtggttgc gggcactggc
     1201 gccgcttctt actgctcttc ggcgatccgc ttccgctccg gtgggatcgc ccagcgttgc
     1261 gctgtcgttc gcggtagctc agtgacgtgc tcgatgtcgc ggtaagacaa gcccatgtcg
     1321 accagagcgg cgattaccac cccaccgtgc tcggcgttgc gctcccgccg gcgcatggct
     1381 tccttgagac cctcgatcag atcggtgcgc aggtcgccgg tgcgttggtc gccgagcagc
     1441 gcttcgaggg cctcgtcggc gtccactgaa accttccttg ccgaggtgtc ccgaaactgg
     1501 gggcattgcg gaacactctt caagctacca agcagcggac ccgaggcgca ccgtacccca
     1561 aaccccaacc cagggagacc gccatggatg agcaatgacc gagtatgacg gcagcgttga
     1621 cggttgtgag caagatgtag cgggcgctgt gatcagatgg cgcgggcaag ttcggtgaag
     1681 accgccggca gccgctcccg tgcttgagct ccaaccccag ttgatggtgc ccacggcgga
     1741 gattctgctc ggcgcgatcg gtgcctcgca cgcgttcatg cagaaccgtc gcggccgtta
     1801 tcttattaca gcaccactac agctacgtct gtccacacat ggtcggtgag tacccgtgag
     1861 aacacgtgga actgtcagcc tccgggcacg gatcggggca tgcagcgggc acgagtccct
     1921 acctgtcccg cttccctgct cttggctgcc ttgctcgttg cggcgatgga gtcaacggtg
     1981 gagctagttg gtgcgtaggc tcgtgaggag atggccgatt ggctgattgt gaggctggga
     2041 gggccagaga ggggtctctt tcgatgagtg agcatgagca gtggcgagag cttgcgcagc
     2101 agttccgtgt cgactccgtc cgcgccagcg gcacggccca gtccgggcat cccacgtcgt
     2161 ccatgtcggc cgccgacctg atggcggtgc tggtgaccaa gtacctccac tacgacttcg
     2221 gcgacgagga caatcccaac aacgatcatc tgatcttctc caagggacac gcctcgcccc
     2281 tcctgtacgc catccataag gcggctggag tggtctccga tgaggagcta ctcacctacc
     2341 gcacactggg ctcccgcttg gagggccatc ccactccgac cgcgccaggg gtcgatgtgg
     2401 ccaccggatc cctgggccag ggcctgcccg tgggcgtggg gctcgccctg tcgggcaagc
     2461 ggctggaccg cctgccctac cgagtctggg tgctgtgcgg cgacagcgag atgaccgagg
     2521 ggtcgatgtg ggaggccttg gaacatgctg gccatgccgg gcttgacaac ctgaccgcca
     2581 tcatcgacgt caaccgcttc ggccagcgag gtaccacgat gcaccagtgg aatctcgacg
     2641 ccttcgcccg gccggccgaa gccttcggct ggcatgtcat taccgtcgat ggccacgacg
     2701 tcggggccat cgacgaggct tacgccgagg cggtcgagac caaggggcag cccacggtga
     2761 tcatcgccaa gaccctcaag ggcaaggggg ttagcgaggt cgaggacacc gagctgcacg
     2821 gcaagccggt caaggatgcg gacaaggcca tcgaggagct gggtggcatt cgtaacatcc
     2881 agatcgctat tcccacgcgt accagcgacg cgaagccgcc ctctttcgag gtcagctcgg
     2941 cggagcggcc tcggtacgag cttggtgagg aggtcgccac ccgcaaggct tatggcgagg
     3001 ctctcgccgc gctcgccagc tgccgaggcg acgtggtcgc actcgacggt gaaatcagca
     3061 actccaccca tgctgacatc ttcgccaagg cccatcccga acgcttcttc gagatgtaca
     3121 tcgccgagca gctcatggtg gccgttgccg tgggcttcca ggtgcggggc tgggtaccct
     3181 atgcctccac gttcgccgcc ttcttgagcc gcgcttatga cttcgtgcgc atggccgcca
     3241 tcagcagggc cagcctcaac ctggtgggtt cgcacgccgg cgtctcgatc ggcgaggacg
     3301 gcccctcgca gatgggcctg gaggatctgg ccatgttccg cgccgtgcac ggcagcaccg
     3361 tcctgcaccc ctgcgacgcc aaccagaccg ctcagctggt ctggcagatg gccgaccggc
     3421 ctggtatccg ctatctccgc accttacgtc cctcgacgcc ggtgatctac ggaccggacg
     3481 agcatttcga gatcggcggc agtcaggtga ttcggcattc cgacggggac caggtcgccc
     3541 tcatcgccgc cggcatcacc gtccacgagg ccctggaggc agccgaggcg ctggaggccg
     3601 agggtgtccg ggcacgggtg atcgactgct actcgatcaa gcccatcgat gctgagacac
     3661 tgcgggaagc ggcccgggcc accggcgggc ggatcctcac tgtcgaggac cattggcccg
     3721 aaggggggct gggcgacgca gtcctcgagg ttttcgccca ggcggaagaa tgcccacgga
     3781 tcgtgaaact ggcggtccgc aagatgcccg gctcaggccc ctccgagaag ctgctgaagg
     3841 acgtcggcat cgatgcgagg gccatcgccg aggaggctcg aaagctcgcc aaggcgacac
     3901 ctcgctagcc gacgatgtca cgcattgccc gatcccatgg tgcacccctc gcgttgacgc
     3961 gttgagcgcg gccccggcgg ttcacccacg ggcccgctcg gtaacactgc cgcgatgacg
     4021 cggggagtcg tggctgccgg gcatccgctg accgctgagg cgggcgcgag cgcgctgcgt
     4081 gcgggcggca acgcggtcga cgccgccctc gccgcgatgt gcgcgtcctt tgcctgcgag
     4141 ccgctgctca cgggcctggg cgccggtggc tacatgcttg tcggagcgcc cggtggggac
     4201 gacgtgctgc tggacttctt cgtggaggcg cccgggcgcg gtggcgaccc gccgcgccgc
     4261 gccgagcttg tgccggtctc ggtcctcttc ggtgacgtgg tccaggtctt ccacgtgggc
     4321 gccgcctccg tcggcaccta cggctgcccg gccgggatct gtgcggcgca cgcgaggttc
     4381 gcctcactgc cgctggcaaa gctggtggcg cccgcagtgc ggctggctcg tgaaggtgtg
     4441 gcgctcaacg cccgtcagac ccacatcatt gagctgctat ggccgatcat caccagctcc
     4501 ccgcaggcgc gcgccttatt cgccaaggca ccgccgcgcg agggcgaggt cctgcgcctg
     4561 ccggatgtgg ccgacggcct ggagcgcctg gcggccgagg gtgcggcgcc gttctacacc
     4621 ggcgagctcg ccacggccgt gtgcgactgg atcggtgagc gcggcggcat gctctcgccc
     4681 gccgacctcg ccgcctacaa ggtggtgcca cgtgagccga tcgaggtccc gtaccgtggc
     4741 ctgcgcgtgc ggacaaaccc cccgccctcg gccggcggca tcctgatcgc ggcggtcctg
     4801 gccgagctgg accgcgagcc ggccccgccc agccgcgagc ggctcgtcgc cgcgatgatc
     4861 gacgcccagg gcgcgcgcac tgcagcgttc atggagagcc tgcacatccg cggctttc
//
LOCUS       DATADN010000046         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_608522, whole genome shotgun sequence.
ACCESSION   DATADN010000046 DATADN010000000
VERSION     DATADN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608522"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..>1668)
                     /locus_tag="VHH53_00605"
     CDS             complement(118..>1668)
                     /locus_tag="VHH53_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2288716.1"
                     /translation="SSFFKQARRRGTKIIYIDPRADRVADHADIYCQLKPGTDVAFYN
                     GVMHEIIRLGLIDREYIASRTSNYDELARTVADYPLQRAAQITGIDAATITEVARVWG
                     NAGAGIIFWGMGISQHTTGTDNARCLIAMCAITGNVGKPGSGLHPLRGQNNVQGASDV
                     GLIPMFYPDYQKADSPQVRARFEQAWRVAGLDPKRGLTVTEIIGSVLTGGVRGMYMLG
                     ENPFLSDPNINKVRKALSALEFLVVQDIFLKETAEFADVVLPASSYLEKNGTYTNTDR
                     RVQLGRKVLDPPGQARVDWEIVQDIAQRIGLNWHYTGAREVFDEMVSLMPNYANLSYD
                     NLGGTGKLYPNAEPEHSDGTVVLFDERFNTDDGLAHLVPAQWLPAKELPDTDYPLVLN
                     TGRLLEHWHTGSMTRRSYALDAIAPQAEVYMHPKDAADRGLAHGEVVRVRSRRGEIEL
                     RLKVSHREALGNCFIPFHFREAAANVLTIDEIDPYGKIPEFKFCAVQVEPLQPAPLGA
                     ELRSEAAR"
BASE COUNT          264 a          540 c          558 g          306 t
ORIGIN      
        1 aagctcggcc cggcaaattt gccggcccga gcctccacac ctggcacctt gtcaccgccc
       61 acaacccgag gctaacgcgg gccattcccg cagccgcagt tcggctccga atggcggtca
      121 gcgggcggct tcagaccgca gttcggctcc gagtggcgca ggctgcaaag gctcgacttg
      181 cacggcgcag aacttgaact cggggatctt cccgtagggg tcgatctcgt cgatggtcag
      241 cacgttggcc gcagcttccc ggaagtggaa cgggatgaag cagttgccga gcgcctcgcg
      301 gtgggagacc ttcaaccgca gctcgatctc tccccggcgg gagcgcaccc gcaccacctc
      361 gccatgcgcg agaccgcggt cggccgcgtc tttgggatgc atgtacacct ccgcctgcgg
      421 tgcgatcgcg tccagggcgt aggaacggcg ggtcatcgac ccggtgtgcc agtgctccag
      481 caaccggccg gtgttgagca ccagcgggta gtcagtgtcg ggcagttcct tggccggcaa
      541 ccactgcgct ggcaccagat gcgccagccc gtcgtcagtg ttgaaccgct cgtcgaagag
      601 caccacggtt ccgtcggagt gttccggctc ggcgttgggg tacagcttgc cggtgccgcc
      661 gaggttgtca taggacaggt tggcgtagtt gggcatgagg ctgaccatct cgtcgaacac
      721 ctcacgggcg ccggtgtagt gccagttcaa gccgattcgc tgggcgatgt cctgcacgat
      781 ctcccagtcg acccgggcct gtcccggcgg gtcgagcacc ttgcgaccga gctgcacccg
      841 ccggtcggtg ttggtgtacg tgccgttctt ctccaggtag gacgacgcgg gcagcaccac
      901 gtcggcgaac tcggcggtct ctttgaggaa aatgtcctgc accacgagga actccagcgc
      961 ggacagcgcc ttgcgcacct tgttgatgtt cgggtcggac aggaacgggt tctcccccag
     1021 catgtacatc ccgcgcacgc cgccggtcag caccgagccg atgatctcgg tgacggtcag
     1081 gccccgcttc gggtccagcc cggccactct ccaggcctgc tcgaaacggg cccgcacctg
     1141 gggggagtcg gccttttggt agtcggggta gaacatcggg atcaggccga cgtcggaggc
     1201 gccctgcacg ttgttttggc cgcgcagtgg gtgcaggccg ctgccgggct tgccgacatt
     1261 gccggtgatc gcgcacatcg caatcaggca acgggcgttg tcggtgccgg tggtgtgctg
     1321 ggagatcccc atgccccaga agatgatccc cgcaccggcg ttgccccaga cccgcgccac
     1381 ctcggtgatg gtggcagcat cgatgccggt gatctgcgcg gcacgttgca gcgggtagtc
     1441 ggcgacggta cgcgccagct cgtcgtagtt ggacgtccgg ctcgcgatgt attcccgatc
     1501 gatcaacccc agacggatga tctcgtgcat caccccgttg tagaacgcca catcggtgcc
     1561 cggcttgagc tggcagtaga tatcggcgtg gtcggcgacc cggtcagccc tggggtctat
     1621 gtagatgatc tttgtgccgc gccggcgggc ctgcttgaaa aatgagct
//
LOCUS       DATADN010000047         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_458098, whole genome shotgun sequence.
ACCESSION   DATADN010000047 DATADN010000000
VERSION     DATADN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458098"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..904
                     /locus_tag="VHH53_00610"
     CDS             197..904
                     /locus_tag="VHH53_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2288717.1"
                     /translation="MFYWLLKHLLLGPLMRLACQPQVYGAEHLPRRGGAILASNHLAV
                     ADSFFLPLVVRRRVTFLAKREYFTTPGLRGWFKRQFFSASGQVPIDRSSGSAAQAALD
                     TGVRLLAAGNLLGIYPEGTRSPDGRLYKGKTGVARMALQAGVPVIPVAMVGTDKVSPR
                     GTKMWRPHRVEVRFGAPLDFSRYAGLAGDRFVERSMVDEIMYSLMELSGQEYVDMYAS
                     SVKEREPPPRLTLKKAS"
     gene            1120..1623
                     /locus_tag="VHH53_00615"
     CDS             1120..1623
                     /locus_tag="VHH53_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyadenylate-specific 3'-exoribonuclease AS"
                     /protein_id="HEX2288718.1"
                     /translation="MRFFYDCEFIEDGVTIELISIGVVDEQGREYYAVSTEFDPGRAG
                     EWVRLHVLPKLPAPSDKAWRSRAAIRDTLLAFLTAPGERVELWAWFAAYDHVALAQLW
                     GPMPALPRELPRFTRDLRQRWEDAGKPALPPPPSDAHDAVADARHNLVRWRVIEEHRS
                     ARSGRTR"
     gene            1707..>1891
                     /locus_tag="VHH53_00620"
     CDS             1707..>1891
                     /locus_tag="VHH53_00620"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX2288719.1"
                     /translation="MRIGVLTGGGDCPGLNAVIRAVVRKGIEVYGHEIIGFRDGWRGP
                     MEGLVQPLDLPDVEHILP"
BASE COUNT          308 a          591 c          661 g          331 t
ORIGIN      
        1 ccactctcgg ctcggctgaa accgttgcag cggcaacggt accgggcggg cggtggtggg
       61 cgagtgcttg cacgattgac cgcgccaacc gaacctgcgt accaggggtg gagcgggatc
      121 gttgtgcgcc cgataccgtg ctccgtaggc ttgccgatgc ggcggcccgc gagggcgaag
      181 gaaggatcga taggcagtgt tttactggct gctcaagcac ctgctactgg gtcccttgat
      241 gcgcctggca tgccagccgc aggtctatgg cgccgagcat ctcccacgcc gcggcggcgc
      301 cattctggcc agcaaccatc tcgcggtggc cgactcgttc ttcctacccc tggtggttcg
      361 ccgccgggtg acgtttctgg ccaagcgcga gtacttcacc acgccgggac tgcggggatg
      421 gttcaaacgg cagttcttca gtgcctccgg acaagttccg atcgaccgat ccagcggctc
      481 ggccgcccag gctgccctgg acaccggagt gcgattgctg gccgcgggca acctgctcgg
      541 catctatcca gaaggcaccc gatccccgga cggccggttg tacaagggca agaccggcgt
      601 tgcccggatg gcgctgcagg cgggcgtccc ggtgatcccg gtcgccatgg tgggcaccga
      661 caaggtcagc cccaggggca ctaaaatgtg gcggccacat cgggtagaag tgcgcttcgg
      721 cgcgccgctg gacttctcgc gctacgcggg tctggccggt gaccggtttg tcgaacgatc
      781 gatggtggac gaaatcatgt actccttgat ggagctctcc ggccaggaat acgtcgacat
      841 gtacgcatcg tcggtcaagg agcgcgagcc accgccgcgg ttgaccctca aaaaggcctc
      901 ctgaccggtc cggtgtcggg tcgtaaccgc gtcttccctg cacctgacca cctgtatgcc
      961 tgctgggggc gtcctgcccg ctgccggaag gggacccctg cccgtaacgg cttgcgggga
     1021 gagcgtcaag acggtgcgca gcggtgcctg ggacactgtg cggacgccgg agattaccgt
     1081 gacatccgcg caggtgagag agtgtgaggg tcggtccagg tgcgcttttt ctacgactgc
     1141 gagttcatcg aggacggcgt caccatcgag ttgatctcca tcggagtggt ggacgagcag
     1201 ggtcgggagt actacgcggt atcgacagag ttcgatcccg ggcgggccgg tgagtgggtg
     1261 cggttgcacg tgttgccgaa gctgccggcg ccgtctgaca aagcatggcg cagccgggcc
     1321 gccatccgcg acacgctgct ggcgtttctc accgcgcccg gtgagcgagt ggagttatgg
     1381 gcctggttcg ctgcatacga tcatgtcgcg ctggctcagt tatggggacc gatgccggcg
     1441 ctgccacgag aactaccccg gttcacccgg gacctgaggc agcgctggga ggacgccggt
     1501 aagccggcat tgcccccgcc gcccagtgac gctcacgacg cggtggccga cgcccggcac
     1561 aacctcgtca ggtggcgggt tatcgaggaa caccgctcgg cgcggtctgg ccggactcga
     1621 taacccctcc cgctttgccg caccaatccc ggtcgtgcca ctctcacgcc gtcaagcagg
     1681 ggacaagtgg aaaggcgaac gacattatgc gtatcggcgt actcacgggg gggggggact
     1741 gcccgggtct caatgcggtg atccgggcgg tggtccgcaa ggggatcgag gtgtacgggc
     1801 acgagatcat cggattccgg gacggctggc gggggccgat ggagggactt gtccagccgc
     1861 tcgatctgcc cgacgtggag cacatcctgc c
//
LOCUS       DATADN010000048         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_458218, whole genome shotgun sequence.
ACCESSION   DATADN010000048 DATADN010000000
VERSION     DATADN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458218"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1392
                     /locus_tag="VHH53_00625"
     CDS             433..1392
                     /locus_tag="VHH53_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2288720.1"
                     /translation="MRVFVAGATGAIGKQLVPRLVAAGHEVHGMTRSESKQAMLYELG
                     AVPVVADALDPDQVAEAVGMAKPDVIVHQLTALAGEGMRGLKHRVAMTNRLRSEGTDH
                     LLSAGQAVGVRRFVAQSNFASYARTGGPVKSEEDPLDPAPPGKLREGVAALRHLEQAV
                     LEAGWTEGIVLRYGGFYGPGTNMAPGGDIYEMIRKRRFPLVGDGGGIWSFIHITDAAE
                     ATMAAVEHGSRGVYNIVDDDPAPVAEWLPILTHMLSGKKPRSMPRFILRLFAGEAGVV
                     MMTEARGASNAKAKRELGWSPAHPSWREGFAAARPSWRQGSAA"
     gene            1389..>1542
                     /locus_tag="VHH53_00630"
     CDS             1389..>1542
                     /locus_tag="VHH53_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX2288721.1"
                     /translation="MTDRERLLDELRPGAFAIAYRMLGSVSEAEDVVQEALLRVDQAL
                     QAGEQIA"
BASE COUNT          263 a          469 c          545 g          265 t
ORIGIN      
        1 cttcgtcgat gaccagcgcc gcggcaggag acgactcacc accccagtct acgacccaaa
       61 cactagatta gttgcgagac acacaccact agcagctcat agcccgatgt gtaggccagc
      121 aggtcgccca agcggttctt cacgaccgcc gcccccggga cagcgtgctc tgggccgatc
      181 gagtggggtc tcgccgcccc tgggcgacga cgcgcttgcc ttcgtgccca atgccgatcg
      241 cgagcgatct tcacgatgcg ttgtgggcga cgttcgtcgt gcaagcgttg ccagccggtc
      301 cccagcattg ccacccccgt gcccgcgttc ccgcgcccgc gttcccatgc cggggtgctc
      361 ggaggtgtca cagccggggc tgccgtcttg tcttgtgggg tgcaggtaaa atcctcgatg
      421 aaggagttga tgatgagagt gtttgtcgca ggagcgacgg gagcgatcgg gaagcagctg
      481 gtgccgcgtc tggttgccgc gggtcatgag gttcatggca tgacgcgtag cgagtcgaag
      541 caggcgatgc tctacgagct gggtgccgtg ccggtggtgg cggacgcgct tgatcctgat
      601 caggtcgcgg aagcggtggg catggcgaag ccggacgtca tcgtccatca gctgactgca
      661 cttgcagggg agggcatgcg tggcctcaag caccgcgtcg caatgaccaa ccgcctgcgc
      721 agcgagggga cggatcacct gttgtcggcc ggacaggcgg tgggggtgcg gcggttcgtg
      781 gcacagagca atttcgcctc atacgcgcgc accggtggac cggtgaagag cgaggaggac
      841 ccactcgacc ccgctccccc gggcaaattg cgcgaaggcg tggccgcgct ccgacacctc
      901 gagcaggcgg tcctggaggc ggggtggacc gagggtatcg tcctgcgtta cggcggcttc
      961 tacgggccag gcacaaacat ggcgcccggc ggtgatatct atgagatgat ccgcaagcgc
     1021 aggtttccgc tggtcggtga cggtggcggc atatggtctt ttatccacat caccgacgcc
     1081 gcggaggcga cgatggcagc ggtcgagcac ggcagtcgcg gtgtctacaa catcgtcgac
     1141 gatgatcccg ccccagtggc cgagtggcta ccgatcctga cacacatgct tagcggcaag
     1201 aagccgagga gtatgccgcg tttcattctg cgactgttcg ctggagaggc cggcgtagtg
     1261 atgatgaccg aggcccgcgg tgcctccaat gccaaggcca agcgcgagct cgggtggagc
     1321 cctgcgcatc cgagctggcg tgagggattt gccgcagcgc gtccgagctg gcgtcagggg
     1381 tctgcggcat gaccgatcgc gagcgactgc tcgacgaact gcggccgggc gcgttcgcga
     1441 tcgcctaccg gatgctcggc agtgtctccg aggccgagga cgtggtgcag gaagcgctgc
     1501 tgcgggtgga tcaggcgctc caagccggcg agcagatcgc at
//
LOCUS       DATADN010000049         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_609041, whole genome shotgun sequence.
ACCESSION   DATADN010000049 DATADN010000000
VERSION     DATADN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609041"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..1052
                     /locus_tag="VHH53_00635"
     CDS             519..1052
                     /locus_tag="VHH53_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX2288722.1"
                     /translation="MTTEAEIRLRLGMIDSRIIVVLLLLIAGIAMLNLLITSGTALWA
                     LLAPVIIPATQLIGVNPATVMAVYRIADSCTNSITPMSSTFILTVGYLQTMRKSAGIG
                     TLVSFTLPAAMAMLLTWTALFGVWYALGNSTWPWITSSITDDAPRSLPAATVPIRYPP
                     RSHHAGRASSGTGAGIP"
     gene            complement(1404..>1835)
                     /locus_tag="VHH53_00640"
     CDS             complement(1404..>1835)
                     /locus_tag="VHH53_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX2288723.1"
                     /translation="DFAESPDDHWRTRRVIDEWLRVVDAPEPSERARLLNDLMAQATA
                     YPRLTDHGREGWHLHYRDDANDSLPDVLRAVISVGTALHLTTRGIHRLSRCAARQMKG
                     DPCAAVVVDVTRNGRQRYCSVRCANRAAVRRHRARRSMTAH"
BASE COUNT          315 a          597 c          604 g          319 t
ORIGIN      
        1 gactctctcg cccagccggg aatggtcacc gagtgtgcct gggttgtggc cttgcgattg
       61 cctcgcggga tcagctagtg gtcgtctgtt ccctagagag caccacgtct ggccgatcgt
      121 cctgccgggc ggtgagcagc gcagcgtaga caacggcggt cacggccgcg cccaagaaga
      181 tgctgacgtc tactccagcg aaccagacga cgaggggacc tgtgtagagg gacgtgctcg
      241 ccgcgcagag cccgacaaca gacccggaca gccatgccac cgttgccgag aggttccagc
      301 cagacgtgta ccagtaccgg ttccacgacc gatcaggcag gaggaaggcc tgcagggcct
      361 cggcgtcgta gcgaccgcgc gtgcagtaga gcccgatgat gtcgatcagg gctcacggcg
      421 ccgtgacgat gaccaagagc gtcgatgccg aaccgattga ctcagcggcg tccatcagga
      481 ttgtgccgat aacgactagc gcaaacgagg ccaccgcggt gaccactgaa gccgagatcc
      541 ggctccgcct cggaatgatc gactcgagga tcatcgtggt cttgctgcta ctgatcgcgg
      601 gcatcgcgat gctcaacctg ctcatcacca gcggcactgc cctatgggca ctcttggccc
      661 cggtgatcat cccggcaact caactcatcg gcgtcaaccc agcgacagtt atggccgtct
      721 atcgcatcgc tgactcttgt accaactcga tcacgccgat gagctcgacc ttcatcctca
      781 ccgtcggata cctgcaaaca atgcgcaaga gcgctggcat cggcacactg gtgtccttca
      841 ccctgccagc tgcaatggcc atgctgctga cctggaccgc actcttcggg gtctggtatg
      901 cactggggaa ttccacttgg ccctggatca ccagttcgat aaccgacgac gccccccgtt
      961 cgctaccggc agcaacggtc ccgatcagat acccgccccg gtctcaccac gctggcagag
     1021 cgagtagcgg caccggcgca ggcattccat gaggatcgcc gtctgcggca ccagtcggtt
     1081 cggttgccga tgccctctgg caggtgcgtc gggcgggcgg agccgggcgg cggcggtgtt
     1141 tgcggtcagg ctctcgcacg cggccgggca tcgggtggcc gtatctcagg agtgggccga
     1201 tcgagaggtc ggccggcacg aggtcgagca cgcctggcga ggatggctcg gcggctgcaa
     1261 gagtgtcggg cagaggagtc ctgaaccgcc ctcaaccgcc gctgtgtgcg cggttcggag
     1321 ggtgggtcat tccgccgcgt actggctacg cacgtgaact gacaggcgct cggccgacag
     1381 gtgcggttcg cgcggcgaat cgatcagtga gcggtcatgg agcgtctggc gcggtgccgg
     1441 cggaccgcgg cgcggttggc gcagcggacc gagcagtagc gctgtcgtcc gttgcgggtg
     1501 acgtccacaa ccactgcagc gcatgggtct cccttcatct gccgcgcagc gcaccggctc
     1561 aggcgatgta tgccccgagt ggtcaggtgc agcgctgtgc ccacgctaat caccgctcgt
     1621 agcacgtcgg gtagcgaatc gttggcgtcg tcgcggtagt ggaggtgcca tccttcccgc
     1681 ccatggtcag tcagccgcgg atacgcggtc gcttgcgcca tcaggtcgtt gagcaaccgc
     1741 gcccgttcac tcggctccgg cgcgtctacg acgcgaagcc actcgtcgat gacccggcgc
     1801 gtgcgccagt ggtcgtctgg cgactcagcg aagtc
//
LOCUS       DATADN010000050         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_759783, whole genome shotgun sequence.
ACCESSION   DATADN010000050 DATADN010000000
VERSION     DATADN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759783"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VHH53_00645"
     CDS             <1..268
                     /locus_tag="VHH53_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2288724.1"
                     /translation="HPMHLHGHHAVVVARDSKKGTGSPIWVDSIDVDDGESVDIAFKA
                     DNPGIWMDHCHNLPHAAEGLVAHLMYEGVTVPYTVGGTAANQPE"
     gene            complement(279..1274)
                     /locus_tag="VHH53_00650"
     CDS             complement(279..1274)
                     /locus_tag="VHH53_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX2288725.1"
                     /translation="MPAVTVEDVTTLARIPKPGPQAVDRPVRSVTTAPSGYEGEGFPV
                     RRAFAGVDLRDLDPFVHMDQMGEVEYAPGEPKGTAWHPHRGFETVTYIIDGIFEHADS
                     HGGGGEITNGDTQWMTAGGGVLHIERPPEHLVASGGLFHGLQLWVNLPARLKWAEPRY
                     QDLRAAESTLLTTTDGGALVRMIAGEVAGHHGPGSTYTPMAMLHATISPAATLRLPWR
                     PDFNALVYVLSGAGTVGAEGRPVRTGQLVVFWPGDVVTVAGAVHQESRHPALDVVILG
                     GAAIREPVAWGGPFVMNTRAEVLQAFEDFQAGRLATIPAERLPHAGVGGEARQDG"
     gene            complement(1353..1838)
                     /locus_tag="VHH53_00655"
     CDS             complement(1353..1838)
                     /locus_tag="VHH53_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288726.1"
                     /translation="MARADLTRVTALDWVVVGTGLLAYIASFLPWYRADVSILGIERS
                     ATANAWNAGIGAWLPVLLLAVAGVVVLASATGVRLPTSKSLITLGLSALAVIAILLRW
                     MTFPDATGQSSLDMDNVDVDGLLAVSSGAGVGLYIGLIAAIVAVVASFLTSRAAGATL
                     S"
BASE COUNT          349 a          714 c          641 g          291 t
ORIGIN      
        1 gcacccgatg cacctgcacg ggcaccacgc ggtcgtcgtg gcgcgcgact cgaagaaggg
       61 caccggcagc ccgatctggg tcgactccat cgacgtcgac gacggcgaga gcgtcgacat
      121 cgcgttcaag gccgacaacc ccggcatctg gatggaccac tgccacaacc tcccgcacgc
      181 ggccgagggc ctggtggcgc acctgatgta cgagggcgtc accgtgccct acacagtggg
      241 aggcactgca gccaaccagc cggagtgacg cagatccgtc aaccgtcttg gcgagcctca
      301 ccgcctaccc cggcgtgcgg caggcgctcg gcggggatcg tggccagccg ccccgcctgg
      361 aagtcctcga aggcctgcag cacctccgcg cgggtgttca tcacgaacgg tcccccccaa
      421 gcgaccggct cccggatcgc agcgccaccc aggatgacga catccagcgc aggatgccgc
      481 gactcctggt gaaccgcccc agcgacggtg acgacatccc ccggccagaa caccacgagt
      541 tgcccggtac gcaccggcct gccctcagca ccgaccgtcc cggcacccga gagcacgtag
      601 accagcgcgt tgaagtctgg ccgccagggc agtcgcaggg tcgctgcggg gctgatcgtg
      661 gcgtgcaaca tcgccatcgg agtgtaggtg gaaccggggc cgtggtgccc ggcgacctcg
      721 ccggcgatca tccggaccag cgcgccgccg tcggttgtgg tgagcagcgt ggactccgcg
      781 gcccgcaagt cctggtaacg gggctcggcc cacttcagtc gcgcaggcaa gttgacccac
      841 agctgcaggc catggaacag cccgccactg gcaaccaggt gctccggcgg ccgctcgatg
      901 tgcagcacgc cgccgcccgc ggtcatccac tgggtgtccc cgttggtgat ctcgccgccg
      961 ccgccgtggg aatcggcgtg ctcgaagatg ccgtcgatga tgtaggtgac ggtctcgaac
     1021 ccccggtgcg ggtgccaggc ggtgccttta ggctccccag gggcgtactc gacctcaccc
     1081 atctgatcca tgtgaacgaa cgggtccaga tcgcgcaggt ccaccccggc gaaggctcgg
     1141 cgcaccggga agccctcgcc ctcatagcca gagggtgcgg tggtcactga ccggactggg
     1201 cggtccacgg cctgtggccc gggctttggg atacgggcta gcgtggtgac gtcctcgacg
     1261 gtgacagcgg gcatggccgc ctccttgatg gttgacccct caactacagg cgaacgctcc
     1321 atcgccgcgc acattccccg cgcgctgggc gctcagctga gggtggcgcc cgcggcgcga
     1381 gacgtcagaa acgacgccac cacggctact atcgccgcga tcagccctat gtagaggccg
     1441 acgccagcac cactggacac ggcgagcaag ccgtccacgt cgacgttgtc catatccagg
     1501 ctgctctggc cagtggcgtc cggaaacgtc atccaccgca gcaggatcgc gatgaccgcc
     1561 agcgcggaca gcccaagcgt gatcagcgac ttcgaggtag gcagtcgcac tcccgtcgcg
     1621 ctcgccaaca ccaccacgcc agccactgcc agcaacagca ccggcaacca tgcgccgata
     1681 cccgcgttcc aggcgttcgc ggtggctgat cgttcgatcc ccaagatcga gacgtcggct
     1741 cggtaccagg gaaggaaact cgcgatatac gcgagcaagc ccgtgccgac gacgacccag
     1801 tccagggccg tcaccctcgt caaatcggct ctcgccatgt tcgaagcctt tcctagacct
     1861 gccgggatcg tagggcctgg ctacggcgtg tctcgcaaat cttcaagcgt gaccctaacc
     1921 ctcggggctc aagagcactc agtgacgccg gcgggtcggt tctgtgtatc aggcggctgc
     1981 gagccggcct cttgg
//
LOCUS       DATADN010000051         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_307611, whole genome shotgun sequence.
ACCESSION   DATADN010000051 DATADN010000000
VERSION     DATADN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307611"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VHH53_00660"
     CDS             complement(<1..429)
                     /locus_tag="VHH53_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2288727.1"
                     /translation="MRTTVGIIGAGPAGLLLARLLHNAGIDSVVLESRDRAYVEHRQR
                     AGILEQGTVDVLRATGAGERMDREGLRHDGIELRFAKRRHRVDFPALTGGRSVMVYAQ
                     TEVCKDLIALQLKEGGPLLFEAEALAVEGADTDSPRVRLRH"
     gene            complement(564..647)
                     /locus_tag="VHH53_00665"
     tRNA            complement(564..647)
                     /locus_tag="VHH53_00665"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(613..615),aa:Leu,seq:taa)
     gene            1109..>1587
                     /locus_tag="VHH53_00670"
     CDS             1109..>1587
                     /locus_tag="VHH53_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2288728.1"
                     /translation="MTTAPLTDRATTVAARATDLSKIYGQGETQVVALDRVSVDFRQA
                     EFTAIMGPSGSGKSTLMHCVAGLDTFSSGSVRIGDTELGSLKDKQLTKLRRDKIGFIF
                     QAFNLLPTLTALENITLPMDIAGRKPDKQWLDSVIRMVGLADRLGHRPSQLSGGQQQR
                     "
BASE COUNT          263 a          535 c          522 g          267 t
ORIGIN      
        1 gtgccggagg cggacgcgcg ggctgtcggt gtcggcgccc tcgacggcca gtgcctccgc
       61 ctcgaagagc agggggccgc cctccttgag ctggagggcg atgaggtcct tgcagacctc
      121 ggtctgggcg tagaccatca cggaccggcc gccggtgagg gcggggaagt cgacgcggtg
      181 gcgccgcttg gcgaagcgca gctctatgcc gtcgtgccgg aggccctcgc ggtccatgcg
      241 ctccccggcg ccggtcgcgc gcagcacgtc cacggtgccc tgctccagga tcccggcgcg
      301 ctggcggtgt tcgacgtagg cgcggtcgcg gctctccagg acgaccgagt cgatgccggc
      361 gttgtggagc agccgggcga ggaggaggcc ggcggggccg gctccgatga tgccgacggt
      421 ggtgcgcatc ggacgccctc ttctcatccg ttcgtgtggt gaaattttct tcaccattat
      481 tggatgcgag tctctgacgg catgcgaccg ctgtcaacgg gtacgccgcg aacttctcaa
      541 tggaccacac agggatcgga aggtgcccgg agccggactc gaaccggcac gcccgcgaag
      601 gggcagcgag gtttaagctc gccgtgtctg cattccacca tccgggcagg ccatgggctc
      661 cgcatcgagg ttccgagact atcgggacgg gtcccccgaa cagcggaagg gcggaccgat
      721 gttgtcttat tttattgacg tctgagggtg catcagcacc aggaacaggc catccgcact
      781 tgccaatagc gttgcgtgtg acacacagtc acgcatgcgg aatgacggaa tttcaccgtc
      841 tgaacaaggg cactccaccc gttcttgaca cggggtcact cggttcttga cagcaggtca
      901 ctcgttttcc ggcgcgttga gcactcgctc ctccgacacc cgttcccctc aggggcgttc
      961 gtcatcccca ggtatgacac cgctcctcgt ggtcagaccg cagtcggccc ccggaaccgg
     1021 aacaacggct gactacacgg cggcgtggga cccggacgat ggaacgagtc catcccagaa
     1081 cgccgccgtc cgacaggagt gccatcccgt gaccaccgca ccgctcaccg accgggccac
     1141 caccgtggcc gcgcgcgcca cggatctgtc gaagatctac ggacagggtg agacccaggt
     1201 ggtcgccctt gaccgggtgt ccgtcgactt ccggcaggcc gagttcaccg cgatcatggg
     1261 cccctccggc tccggcaagt cgacgctgat gcactgtgtg gccggtctcg acaccttctc
     1321 ctccggatcg gtgcgcatcg gcgacaccga gctcggctcc ctgaaggaca agcagctgac
     1381 caagctgcgc cgggacaaga tcggcttcat cttccaggcg ttcaacctgc tgccgacgct
     1441 gacggccctg gagaacatca ccctcccgat ggacatcgcg ggccgtaagc cggacaagca
     1501 gtggcttgac tccgtcatcc ggatggtcgg tctggcggac cgactcggcc accggccctc
     1561 ccagttgtcc ggcggccagc agcagcg
//
LOCUS       DATADN010000052         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_157476, whole genome shotgun sequence.
ACCESSION   DATADN010000052 DATADN010000000
VERSION     DATADN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157476"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VHH53_00675"
     CDS             complement(<1..423)
                     /locus_tag="VHH53_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288729.1"
                     /translation="MTVYASDPIPRPAGRPASATPLRDHLALATLGVNEGYVVLPRSL
                     AEEMPLPWQQQMAYLIDELHQAYGDLPWPIYRVVPSRTERLVDLDEGQLAEAGYLLEI
                     DASGELVYRDRDDTVVTDPQSREVLVSCLDPIPRRPSQA"
     gene            complement(433..1440)
                     /locus_tag="VHH53_00680"
     CDS             complement(433..1440)
                     /locus_tag="VHH53_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX2288730.1"
                     /translation="MRRWLIGLLVVGLLGALIAVSAISVLLGGVGSGDWRFGVPCGSV
                     LASTDPREAPQVAVQVSDLDGEQRAIVDLIIAIGKQRGLPPRAWQIAIQAGMTESRLH
                     NLTYGDRDSLGIFQMRPSMGWGSVTQVTDPVYQINKFYDVLLTVPNWETRRPGDIAQA
                     IERSAFPLRYHQWEPLAVNVISAVGGNVAEFVPASCVTPLPAPSEVAGRAMQFALGEV
                     GKPYVWGATGPHSYDCSGLMLRAYESTGVTLPRVSRQQYWAGAQLPVRQAQPGDLLFW
                     AYDTSNPDSIHHVAMYLGNGQMVEAANQTVPLRQRAVSFDEYELMPLAVRPGVPVAAE
                     A"
     gene            complement(1444..>1776)
                     /locus_tag="VHH53_00685"
     CDS             complement(1444..>1776)
                     /locus_tag="VHH53_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288731.1"
                     /translation="PPSFPSTPAPTEALFVAKSWVGAFLTVPDGIDSVRWAEQLKPYT
                     TDELLPELRSIDPANVPEATIAGEPRTISSGASSAEIDVPTTGVVMRLLLVSTPAGWR
                     VAGYQRAG"
BASE COUNT          315 a          607 c          587 g          267 t
ORIGIN      
        1 cgcttgggag ggacggcggg gtatcgggtc caagcatgag actaggacct cccgggactg
       61 tggatcggtg accacggtgt cgtcgcgatc ccggtacacc agctcaccgc tggcgtcgat
      121 ttctagcagg tagccggcct cggcgagctg gccctcatcg aggtcgacca accgttcggt
      181 gcggctgggc accacccggt agatgggcca cgggagatcc ccgtaggcct ggtgcagctc
      241 atcgatcagg taggccatct gctgctgcca aggcagcggc atctcctcgg ccaacgaccg
      301 tggcagcacg acatagccct cattgacccc gagcgtcgcc agcgcgagat gatcccgtag
      361 cggagtggcc gaagccggcc gaccagccgg tcgcggaatt gggtccgacg cgtacaccgt
      421 cacctccgga tatcaggcct ccgcggcgac cggcaccccg ggccggaccg cgagcggcat
      481 caattcgtac tcatcgaagc tcacggcccg ctgccgcagc ggcaccgttt ggttggccgc
      541 ctcgaccatt tgaccgttac ccaggtacat cgcaacgtgg tgaatgctgt ccgggttcga
      601 ggtgtcgtaa gcccagaaca gcaagtcccc gggctgtgcc tggcgcaccg gcaactgtgc
      661 accggcccag tattgctgtc gcgacacccg aggcagcgtg acccccgttg actcgtaggc
      721 ccgcaacatc agtccggagc agtcgtaaga atgggggccg gtggcgcccc agacgtaagg
      781 cttgccgacc tcgccaagcg cgaactgcat cgccctcccg gcgacctcgg agggcgccgg
      841 gagcggcgtc acacaggagg cagggacgaa ctccgcgacg ttgccgccca cggcgctgat
      901 cacgttgacg gccagcggtt cccactggtg gtagcgcaac gggaaggcgg atcgctcgat
      961 ggcctgcgcg atgtcccccg gccgccgggt ttcccagttg ggcaccgtca gcagcacgtc
     1021 gtagaacttg ttgatctggt agacgggatc ggtgacctgg gtgacactgc cccagcccat
     1081 cgaggggcgc atctggaaga ttcccagcga gtcccggtcg ccgtaggtga ggttgtgcag
     1141 ccgcgactcc gtcatccccg cctggatggc gatctgccac gctcgtggcg gcaacccgcg
     1201 ctgcttaccg atcgcgatga tcaggtcgac gatggcccgt tgctcgccgt cgaggtcgct
     1261 gacctgcacg gccacctgtg gcgcctcgcg gggatcggtg gacgcaagca ccgagccgca
     1321 cggcaccccg aacctccagt cgccggaacc gaccccgccg agcaggacgg agatcgcgga
     1381 caccgcgatg agagcaccga gcaaaccgac gaccaggagc ccgatcaacc atcgccgcac
     1441 ggctcaaccc gcccgctggt acccggccac ccgccacccg gccggggtgg agaccagcag
     1501 cagccgcatc accaccccgg tggtcggcac atctatttcg gctgaactcg cgcccgagct
     1561 gatcgttcga ggttcaccag cgatcgtcgc ctcgggaaca ttggccgggt cgatagaccg
     1621 cagttcgggg agcaactcat cggtggtgta gggcttcaac tgctcggccc atcggaccga
     1681 atcgatgccg tccgggacgg tcaagaacgc gcccacccag gacttcgcaa cgaacagggc
     1741 ctcggtcggg gccggagtgc tggggaaaga aggcgg
//
LOCUS       DATADN010000053         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1363366, whole genome shotgun sequence.
ACCESSION   DATADN010000053 DATADN010000000
VERSION     DATADN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363366"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..670)
                     /locus_tag="VHH53_00690"
     CDS             complement(227..670)
                     /locus_tag="VHH53_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2288732.1"
                     /translation="MSAHVLPSGGGRTFGPGINVKIEYGQSDGFAVFESELPPASDGP
                     PPHLHRVYDEAFYVIAGNVAFSVDSDTRDCPTGSFVFVPRGVAHGFANPAGEPARILV
                     ITSPGAIQLVEAIYDLLSRGGPPDFEAMAALYASHRSEILGAPSQ"
     gene            complement(845..1144)
                     /locus_tag="VHH53_00695"
     CDS             complement(845..1144)
                     /locus_tag="VHH53_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288733.1"
                     /translation="MHARVSSYQSSPEQVTDAAVDNMNANLLPRVLEMDGNRGAIFLL
                     DRETGKTMTITLWDSEEAMRASEEKGNQLRRDTAQTTSASIGNIERFEVASMELR"
     gene            complement(1338..1526)
                     /locus_tag="VHH53_00700"
     CDS             complement(1338..1526)
                     /locus_tag="VHH53_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288734.1"
                     /translation="MPALGWTATTLSGIAVDKQFTVFGAYRAVTLSARSGLLLPDRPA
                     AGSPATPAPTPAPSEAQA"
     gene            2210..>2615
                     /locus_tag="VHH53_00705"
     CDS             2210..>2615
                     /locus_tag="VHH53_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288735.1"
                     /translation="MITRRQMRAVLADGGQVVDGAGRSVGRIVHVLLDVRTVEPAHAT
                     VACVRCDGVAVVPLARAWLVDGCLHVPYTAAEVCGAPRADGSAGRLDPQLVEELDRYY
                     ARLNDGALVAPNGNGEGGRIASLTLAPAERGTP"
BASE COUNT          386 a          898 c          914 g          417 t
ORIGIN      
        1 ggacgggtgg gtccggccgt gggtcgccct ccgccggtgg gtcagaagct ggtgggtgca
       61 tgccgggtgg tcgggtggtg cgggtgaccc ccgagggtgt ggtgacgtgc agggtgccgt
      121 cggggtccat gacgaaccgc cagccgtggg cgtgggtctt catggcatga agggcctact
      181 ggtgacccga aggacagagg tctagcagag gtcccgtccg actggcctac tgcgacggtg
      241 caccgaggat ctcggatcgg tgactggcgt acagcgccgc catcgcctcg aagtcgggcg
      301 ggccgccacg actcaacagg tcgtagatcg cctcaaccag ttggatggcc ccgggagagg
      361 tgatcaccag gatccttgcc ggctcaccag ccggattggc gaatccatgt gccacgccac
      421 gtgggacaaa cacgaaggat cccgttggac agtcccgggt gtcgctgtcc accgagaacg
      481 cgacgtttcc ggcgatcaca tagaaggcct cgtcatacac ccggtgcagg tggggcggcg
      541 gaccgtccga cgcgggcggc agttcggact cgaacacagc aaagccgtca ctctggccat
      601 actcgatctt gacgttgatt cccggaccga aggtgcgacc accgccgctg ggaagaacat
      661 gagctgacat gcgggctcca accggcagct gcagcttgcg ggtatgcctt cgtacgtctg
      721 accgtctcca gggtcaaccc tgccgccaca caggacatgg gcccgtcgcc acgtcacccg
      781 gaggaccgtc ccccacggca ccaactgtgg ctgacggggc cggtcgtgac agcttgatcg
      841 gctctcagcg cagctccatc gacgccactt cgaaccgctc gatgttcccg atgctggcgc
      901 tcgtggtctg cgctgtgtct ctgcggagct ggttgccctt ctcctccgag gcccgcatgg
      961 cctcctcgct gtcccacaac gtgatggtca tcgtcttccc ggtctcacga tccagcagaa
     1021 agatcgcgcc gcggttgccg tccatctcca acacccgcgg gaggaggttg gcattcatgt
     1081 tgtccaccgc cgcgtcggtc acctgctccg gcgagctctg atagctgctg acgcgagcgt
     1141 gcacgaagca cctccgacag tgtcggcctc tggggcgtcg tacgttcctc gtcgtctggg
     1201 tcctgacaca agagccttgg cccggcagcc accaagccgc gggagtccag tcgatgcttg
     1261 acctccgcag cgaagcacta ccagcacggc caaaccgatg gcgccggtgc cgaagatgac
     1321 gtgctgcggg gcggcggtca tgcctgggcc tcgctcggtg ccggcgtcgg tgcgggtgtc
     1381 gcaggggagc ccgctgccgg gcggtcgggt aggagcagcc cggaccgggc ggacaaggtc
     1441 actgctcggt aggcgccgaa gacggtgaac tgcttgtcga cggcgatccc gctgagggtg
     1501 gtggcggtcc agcccagcgc gggcatcgcg gcagcccctg cgaggcgtgg gtgctcgacg
     1561 ccatcacctg accgcctgcg accccacccg gctccgggcg atcggcaccg cctaccgccg
     1621 ggcgctgcga cacgaccggt ggatgaccct gaccgggacc cccacccttc ctgcagcagc
     1681 aactggcccg cctacgcctc caccaccccc cagtccgcac gacgggctgc acggccatcg
     1741 aggccgtctt ccgtgtgatg acctcctcat gcgagccgcg gtggcggcgc ccgacgccca
     1801 gtgatgcgac ggcgcgaatc acgctggatg ggagttggtt gcagtgccgg cacgatcccg
     1861 gtgatgcgct gtgcccggcc aacgctgctc agtccagccg tacctaccgt gacgccggct
     1921 gccgggaggt cacccgacgc gtaggcttca tcttgggagc ggctgcagcc gctcatgggt
     1981 ggccccggac ccggcgacct caccggttac ctggggagaa ctccgtgcgc gcctctgttg
     2041 cctccaccgt cgcaggccct gcgtcaggtc catcggtccg cgccggaacc ccccgcccgt
     2101 cgtcgccacg ggatcgctgc aactggtcgc aggaaccgcc caggcctgtg cgcgctgacc
     2161 ggctcgccgc ccacggcgcc gaccggtcca gcgcggccgg gaggacacgg tgatcacccg
     2221 gcgacagatg cgcgctgtgt tggccgacgg agggcaggtg gtcgacggcg ctggtcggtc
     2281 cgtgggccgc atcgtgcacg tgctgctgga tgtgcggacg gtcgagcctg cccacgcgac
     2341 cgtcgcctgc gttcggtgtg atggggtcgc agtcgtgccg ctcgcccggg cgtggttggt
     2401 cgacgggtgc ctccacgtgc cctacaccgc ggccgaggtg tgcggcgctc cccgcgcgga
     2461 tgggtccgcc gggcgcctcg atccgcaact ggtggaggag ctcgaccggt actacgcacg
     2521 cctgaacgac ggtgccctgg tggcgccgaa cgggaacggc gaggggggca ggatcgcgag
     2581 cttgacgctt gcaccggccg agcgggggac gccgg
//
LOCUS       DATADN010000054         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_308748, whole genome shotgun sequence.
ACCESSION   DATADN010000054 DATADN010000000
VERSION     DATADN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308748"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VHH53_00710"
     CDS             complement(<1..828)
                     /locus_tag="VHH53_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2288736.1"
                     /translation="MGAGTVRLPDPGGQGDQLRDGGAALTGYLLRRTGQSLVVLVGVS
                     VIVFSLVHLVPGDPVRLALGTRFSQESYDALRERNGLDLPLVEQYFRWAGRAVTGDLG
                     VSFRSGETVTSLIAERLPATLTLAFAALGVALVIALPLGMLSALHPRSFVDRLATVVS
                     QFGISVPDFWLSIMLILVFAGTLGWLPSGGYVPLTEDPLGWLQRLVLPAVATGVVSGS
                     VITRFVRSSVLEALGQDHVRTAQAKGVPTRQVLTWHVLRNALLPLVTVTGVQLAYLLS
                     "
     gene            complement(753..>1713)
                     /locus_tag="VHH53_00715"
     CDS             complement(753..>1713)
                     /locus_tag="VHH53_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2288737.1"
                     /translation="ANGTGPFQLESSNASRTELTAYEDHWGGAPSIDGVEFRYITEPA
                     AALTALQNGEVQWTDNIPPQQIESLSGDDSVELQTTPSVDYWYLSMNYDRAPFDDRDV
                     RRAISFAVDRPAVAEAARFGAAQPNQTAIPEDNFFYTDYAPFTPDVDQARQLLEQAGV
                     TTPLTMGLMVTDEFPETVTAAQVIAGQLEQIGITVEIETLDFATWLDRQGQGDFDAFL
                     LGWLGNLDPAAYYQEQHQTDAANNYQGYSNPQVDQLLQQGATETDQDARKQVYDQAAR
                     IIVDDVSYLYLYNPDVVQGWVPGLSGYQIRADKAINFETVELP"
BASE COUNT          253 a          594 c          617 g          249 t
ORIGIN      
        1 cgacagcagg taggccagct gcacgccggt gacggtgacc agcggcagca gcgcgttgcg
       61 cagcacgtgc caggtgagca cctgccgggt ggggacgccc ttggcctgcg cggtgcgcac
      121 gtggtcctgt cccagcgcct cgagcacgct ggaccgcacg aagcgggtga tcaccgagcc
      181 ggacaccacg ccggtggcca ccgcgggcag gaccagccgc tgcagccagc ccagcgggtc
      241 ctccgtcagc gggacgtagc cgcccgaggg cagccagccc agcgtcccgg cgaagaccag
      301 gatgagcatg atcgacagcc agaagtccgg caccgagatg ccgaactggc tgacgacggt
      361 ggccagccgg tcgacgaacg accgcgggtg cagcgccgac agcatgccca ggggcagcgc
      421 gatgaccagc gccacgccca gcgcggcgaa cgccagggtc agcgtcgccg gcagccgctc
      481 ggcgatgagc gaggtgacgg tctccccgct gcggaagctc accccgaggt ccccggtgac
      541 cgcccgtccc gcccaccgga agtactgctc gaccagcggc aggtccaggc cgttgcgctc
      601 ccgcagcgcg tcgtaggact cctgcgagaa ccgggtgccc agggccagcc ggaccgggtc
      661 gccgggcacc aggtgcacca gcgagaacac gatcacgctg accccgacca gcaccaccag
      721 cgactggccg gtgcgccgga gcaggtaccc ggtcagggca gctccaccgt ctcgaagttg
      781 atcgccttgt ccgcccggat ctggtaaccg gacagtcccg gcacccagcc ctgcacgacg
      841 tcggggttgt agaggtacag gtacgagacg tcgtccacga tgatccgcgc ggcctggtcg
      901 tagacctgct tgcgggcgtc ctggtcggtc tcggtcgcgc cctgctggag cagctggtcg
      961 acctgcgggt tgctgtagcc ctggtagttg ttcgccgcgt cggtctggtg ctgctcctgg
     1021 tagtaggcgg ccgggtcgag gttgcccagc cagccgagca ggaaggcgtc gaagtcgccc
     1081 tggccctgcc ggtccagcca ggtggcgaag tccagcgtct cgatctccac ggtgatgccg
     1141 atctgctcga gctggccggc gatcacctgc gccgcggtga cggtctccgg gaactcgtcg
     1201 gtgaccatca gccccatcgt cagcggcgtc gtcaccccgg cctgctccag cagctggcgg
     1261 gcctggtcga cgtcgggggt gaacggcgcg tagtcggtgt agaagaagtt gtcctcgggg
     1321 atcgcggtct ggttcggctg ggccgccccg aacctcgccg cctcggcgac cgccgggcgg
     1381 tcgacggcga aggagatcgc gcgccggacg tcgcggtcgt cgaacggcgc ccggtcgtag
     1441 ttcatggaca ggtaccagta gtcgacgctg ggcgtggtct gcagctccac cgagtcgtca
     1501 ccggacagcg actcgatctg ctgcggcggg atgttgtcgg tccactgcac ctcgccgttc
     1561 tgcagcgcgg tcagcgcggc ggccggctcg gtgatgtagc ggaactcgac gccgtcgatg
     1621 ctcggcgcgc cgccccagtg gtcctcgtac gcggtgagct cggtcctgct ggcgttggag
     1681 ctctccagct ggaacgggcc ggtgccgttg gcc
//
LOCUS       DATADN010000055         2030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_911862, whole genome shotgun sequence.
ACCESSION   DATADN010000055 DATADN010000000
VERSION     DATADN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911862"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..805
                     /locus_tag="VHH53_00720"
     CDS             317..805
                     /locus_tag="VHH53_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX2288738.1"
                     /translation="MSVTDELLTNNARYTETFSGPLPLPPAKGVAVVACMDARLDVYR
                     ILGLNEGEAHVIRNAGGVVTDDEIRSLAISQRLLGTREIILIHHTDCGMLTFTDDQFK
                     KSIQDETGIKPEWAAEAFPDLDEDVRQSIARIKASPFVPHKDSIRGFVFDVGTGKLNE
                     VV"
     gene            complement(869..1810)
                     /locus_tag="VHH53_00725"
     CDS             complement(869..1810)
                     /locus_tag="VHH53_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX2288739.1"
                     /translation="MNAREVGLPDRHPGDGLPQRVTIWEVGARDGLQNESVVVPVEIK
                     AEFLDRLAGAGLAVLEATSFGHPRWVPQLADAATLFELLARRDGVRYPVLVPNEQGLD
                     RALAVGVTDIAVFASATDSFARRNLNRGLDEQFTMFSPVISRATAEGLQVRGYVSMCF
                     GDPWEGAVAVQQVVDVGRRLLDLGCHQLSLGDTIGVATPGHVAAVLNGFAGAGIETDA
                     LAVHFHDTYGQALANTLAALHAGVTCVDASAGGLGGCPYAESATGNLATEDLVWMLDG
                     LGIGHGVDLDALVRTSVWMAGKLGRPSPSRVVRALSR"
     gene            complement(1800..>2030)
                     /locus_tag="VHH53_00730"
     CDS             complement(1800..>2030)
                     /locus_tag="VHH53_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2288740.1"
                     /translation="LVREGILSRSGIDIPINRINSVQFRHNVIERLLGCGTLIIESAA
                     DEPLEFDDIPQVERVHSLLYYDVTTGPWTSER"
BASE COUNT          370 a          720 c          628 g          312 t
ORIGIN      
        1 aggagaagaa ctcacgacca tgctggacga cttacgagag aagctgtaaa gagccagggt
       61 cggtgaggtc agcctaggag tgctgcgagg cacctgagtc gccgaatctc ttgcccatac
      121 ggcgaacctc gggtaacgat atgcgcatgc ggcgcgctgt gctcgagcag ttctggccga
      181 gccggcgcat ggacacgttc gatcaattct gtagctaggc cgcgcgtcgg cgcatggcct
      241 ccccgccccg cgcggccgat cccggtgccc cctgctcatc cccttgcacc agtatccccg
      301 caccccagga gagttcgtgt ccgttacaga tgaactgctc accaacaacg cccgctacac
      361 cgaaaccttc tccgggccac tgccgctgcc tcctgccaag ggcgtcgccg tcgtggcctg
      421 catggacgcc cgcctggatg tctaccgtat cctgggcctc aacgagggcg aggcacacgt
      481 gatccgcaac gcgggtggcg tggtcaccga tgacgagatc cgctcgctgg cgatcagcca
      541 gcggctgctc ggcacccggg aaatcatcct catccaccac accgactgcg ggatgctcac
      601 ctttaccgac gaccagttca agaagtcgat ccaggacgag accggcatca agccggagtg
      661 ggccgctgag gccttcccgg acctcgacga ggacgtccgg cagtccattg cccggatcaa
      721 ggccagtccg ttcgtgccgc acaaggactc tatccgcggg ttcgtcttcg acgtggggac
      781 tggcaaactc aacgaagtcg tgtgagctaa ccatctctgc gagccctgcc ggtgcggttc
      841 ggcagggctc gcagccctcg gggcaatgtc acctgctcag cgcccggacc acccgagacg
      901 gtgacggtct gcccagtttc cccgccatcc acacgctggt ccgcaccagg gcgtcgaggt
      961 cgaccccgtg tccgatacca agcccgtcca gcatccaaac caggtcctcg gtggcgaggt
     1021 tgccggtggc cgactcggcg taggggcagc cgcccagccc accggccgag gcgtccacac
     1081 aggtcacccc ggcatgcagg gcggccagcg tgttggccag cgcctggccg taggtgtcgt
     1141 ggaagtgcac cgcgagcgcg tcagtctcga tgcccgcgcc ggcgaacccg ttcagcactg
     1201 cggccacatg gcccggggtg gccaccccaa tggtgtcgcc cagggagagc tggtggcagc
     1261 ccaggtccag cagccggcgc ccgacgtcga caacctgctg cacggccaca gcgccctccc
     1321 acgggtctcc gaagcacatc gagacgtagc cgcgcacctg cagaccctcg gctgtagctc
     1381 ggctgatcac cggcgagaac atcgtgaact gctcatcaag gccacggttg agattgcgcc
     1441 gggcaaagct atcggtggcc gaggcgaaga ccgcgatgtc ggtgacaccc accgccaaag
     1501 cccgatccaa cccctgctcg ttgggcacca gcaccgggta acgcaccccg tcgcggcgcg
     1561 cgagcagctc gaacaacgtc gcggcgtcgg ccagctgcgg cacccagcgt gggtgcccga
     1621 aactggtcgc ctccaagacc gccagcccgg ccccggccaa ccgatccagg aactcggcct
     1681 tgatctcgac ggggaccacc acggactcgt tctgcaagcc gtcgcgcgcg ccaacctccc
     1741 agatcgtcac ccgctgcggc aacccgtcgc cagggtgacg gtcgggaagg ccaacctccc
     1801 tagcgttcac tggtccacgg tccggtggtc acgtcgtagt agagcagcga atgcacccgc
     1861 tcgacctggg ggatgtcgtc gaattccagc ggctcgtcgg cggccgactc gatgatcagc
     1921 gtgccgcaac ccagaagccg ttcgatgacg ttatgccgga actgcacact attgatccgg
     1981 ttgatcggga tgtcgatacc gctgcgcgac aagatgccct cgcggaccag
//
LOCUS       DATADN010000056         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1213790, whole genome shotgun sequence.
ACCESSION   DATADN010000056 DATADN010000000
VERSION     DATADN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213790"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..104
                     /locus_tag="VHH53_00735"
     CDS             <1..104
                     /locus_tag="VHH53_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX2288741.1"
                     /translation="YRRLTIRYERSARLFTAFLTLAAALTCYKKLAT"
     gene            complement(122..1015)
                     /locus_tag="VHH53_00740"
     CDS             complement(122..1015)
                     /locus_tag="VHH53_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEX2288742.1"
                     /translation="MRTDLNTLLTALYVWIDDYLGPRRRAGRPPRLTDAELLTLAVAQ
                     ALLDVRSEARWLRLIPQRLPGAFPYLPDQSGYNKRLRGAVPLLRRVIRDLAAATDLWT
                     DPVWLVDSTPVECARSRPAARRSDLAGAAGYGYCANHSRYFWGLRLHLVCTPAGLPIT
                     WALAHPKLDERQVLMAALDHDAHLLTARPGLLIIADKGYASADLDDYLHARGVELLRP
                     AYRNRAARPGQALLAPVRQLIESVNDTLKGQLDLELHGGRSLTGVTARIAQRLLALTA
                     AIWHNRATGQPITRSLIAYDH"
     gene            complement(1118..>1898)
                     /locus_tag="VHH53_00745"
     CDS             complement(1118..>1898)
                     /locus_tag="VHH53_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX2288743.1"
                     /translation="VTDGAMSKSLEKLSSGLRINRAADDAAGLAISEGLRAQIGGLKV
                     AVRNSQDGISVVQTAEGALTETHSILQRMRDLAVQASNHGALTDADKAKADEEFQALA
                     KELDDIASKTTFNGTKLLNGSYSSMNFQVGSNAGETLTVSIGNMAASGASSLFGATGA
                     GNISSASSASSAISAVASAIGTVSTERAKLGAVQNRLEHKINNLNATIENLTASESRI
                     RDTDMAEEMVSFTRSQILSQAGTAMLAQANQSSQGVLQLLR"
BASE COUNT          269 a          613 c          713 g          303 t
ORIGIN      
        1 gctaccgccg attgaccatt cgctacgaac gctccgctcg gctgttcacc gcattcctta
       61 ccctcgccgc tgcgctcacc tgctacaaga agctggccac gtgagacaag ctctaagccg
      121 gtcagtggtc gtaggcgatc agggaacggg tgatcggctg gccggtggcc cggttgtgcc
      181 agatcgcggc ggtgagcgcc agcaggcgtt gcgcgatccg ggcggtcacg ccggtgaggc
      241 tgcggccgcc gtgcagttcc aggtcgagtt gccccttgag ggtgtcattg acggactcga
      301 tgagctggcg gaccggggcc agcagcgcct ggccgggacg ggccgcgcgg ttgcggtagg
      361 ccgggcgcag cagttccacg ccgcgggcgt gcagatagtc gtcgaggtcg gcggaggcat
      421 agcccttgtc ggcgatgatc agcagccccg gccgggcggt gagcagatgg gcgtcgtggt
      481 cgagcgcggc catcagcacc tggcgttcgt cgagcttggg atgcgccagc gcccaggtga
      541 tcggcagccc ggccggggtg cagaccaggt gcagccgcag cccccagaag tagcgggagt
      601 ggttggcgca gtagccgtag ccggcggcgc cggccaggtc cgagcgccgg gcggcggggc
      661 gggagcgggc gcactccacc ggggtggagt cgaccagcca gaccggatcg gtccacaggt
      721 cggtggcggc ggccaggtcc cggatcaccc ggcgcagcag cggcaccgcg ccgcgcagcc
      781 gcttgttgta gcccgattgg tcgggcagat agggaaacgc cccgggcagc cgctggggga
      841 tcagccgcag ccagcgcgcc tcggagcgga cgtcgagcag cgcctgggcc acggccaggg
      901 tgagcagctc ggcgtcggtc agccggggcg ggcgaccggc gcgacggcgc gggcccagat
      961 agtcgtcgat ccagacatag agtgcggtca ggagggtgtt taggtcggtc ctcacaaccc
     1021 gagcctgaac accctccgcc cacgtctaca gcgccctccg accctccggg tcgttaggac
     1081 ttactcgtct aggcgctcag gcgctcgggg accgaggtca ccggaggagc tgcaggacgc
     1141 cctgcgagct ctggttcgcc tgggccaaca tcgccgtacc ggcctgagac aggatctgcg
     1201 accgggtgaa ggacaccatc tcctcggcca tgtcggtgtc gcggatgcgg ctctccgacg
     1261 cggtgaggtt ctcgatggtg gcgttcaggt tgttgatctt gtgctcgagc cggttctgca
     1321 ccgcgccgag cttggcgcgc tcggtggaga cggtgccgat ggcgctggcg accgcgctga
     1381 tcgcggagct ggcggaggag gccgaggaga tgttgccggc gccggtggca ccgaacagcg
     1441 agctcgcgcc gctggcggcc atgttgccga tgctgaccgt cagcgtctcg ccggcgttcg
     1501 acccgacctg gaagttcatc gacgagtagc tgccgttgag cagcttggtg ccgttgaacg
     1561 tggtcttgct cgcgatgtcg tcgagctcct tggccagggc ctggaactcc tcgtccgcct
     1621 tggccttgtc ggcgtccgtg agggcaccgt ggttggacgc ctgcaccgcg aggtcgcgca
     1681 tgcgctgcag gatggagtgc gtctcggtga gcgcaccctc agcggtctgg acgaccgaga
     1741 tgccgtcctg cgagttgcgg acggcgacct tgaggccacc gatctgggcc cgcaggccct
     1801 cggagatcgc caggccggcc gcgtcgtcgg cggcccggtt gatgcgcaga ccgctggaca
     1861 gcttctccag cgacttgctc atcgcaccgt cggtcacg
//
LOCUS       DATADN010000057         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1062979, whole genome shotgun sequence.
ACCESSION   DATADN010000057 DATADN010000000
VERSION     DATADN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062979"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..337)
                     /locus_tag="VHH53_00750"
     CDS             complement(2..337)
                     /locus_tag="VHH53_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288744.1"
                     /translation="MTRLRAGFLLLLFLAAGTSLPSLDALLYHGAGAETQKAQNHLEP
                     TGGCLDHDGHCGLGRTAPGTGAVAPRGDAPLLKHDHGAASPLKPGQSGPSPLHDVLPP
                     PRAPPASRV"
     gene            complement(386..>1631)
                     /locus_tag="VHH53_00755"
     CDS             complement(386..>1631)
                     /locus_tag="VHH53_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2288745.1"
                     /translation="VAKAAAELIEAESGAVTLVVEEGRFLKVKAATGALEQAIGMLVP
                     MDGSLLGWAVTQDAAVVSDDMNADPRSYQPPTFTVPLKTAAIVPLRSAGVVVGTVSVY
                     NHRDGRPFGEHDLNLLQILGDQVVVGLDRASVLAESRRNELALRTKNQELQRATQLKS
                     EFLTNMSHELRTPLNAIIGFSDLLLEGGAGDISPQQREFTEAVLRNGKHLLSLINSVL
                     DLSKVEAGRMSLSLATTDLREAITGAVADTASLRTAKQQECILRLDNDPLTVVADGVR
                     IRQILFNLLSNASKFTPEGGKISLAAVRTRAPLKLPADRAGDQARLLPQEVVWISVTD
                     EGVGVKREDMPKLFQEFSQVDSSASRRAQGTGLGLALCKKFVEMHGGTIGADSIYGKG
                     AAFWFLLPVDGPVRRSTAERSA"
BASE COUNT          253 a          535 c          562 g          281 t
ORIGIN      
        1 ctcagacgcg cgatgcggga ggagctctgg gtggcgggag aacgtcgtga agtgggctcg
       61 gcccggactg gcccggcttg agtggcgaag cagccccgtg gtcgtgcttt agcagcggcg
      121 catcgccgcg cggggccacc gctcccgtgc cgggtgcggt gcggccgagg ccgcagtggc
      181 catcgtggtc gagacagccg ccggtcggct cgaggtggtt ctgcgccttc tgggtctcgg
      241 caccggctcc gtggtagagc agggcgtcga ggctgggcag gctggttcct gcagccagga
      301 agaggaggag caggaaaccg gctctcaatc gagtcacggt cgaattataa actccggagc
      361 ttccggctct gcagcccggc cttgcttagg cgctgcgctc ggcggtggag cgacgcaccg
      421 gaccatccac cgggagcaga aaccagaagg cggcgccttt cccatagatc gagtcggctc
      481 cgatggtccc gccgtgcatc tccacgaact tcttgcagag ggccaatccg agtccggtgc
      541 cctgcgcgcg gcggctggcc gagctgtcca cctggctgaa ttcctgaaac agcttgggca
      601 tgtcttcccg tttgaccccg acaccctcat cggtcaccga gatccagact acctcctggg
      661 gcagcaggcg ggcctggtct cccgcgcggt cggccggcag cttgagcggg gcacgagtgc
      721 gcaccgcggc gagggagatc ttgccgccct cgggagtaaa cttgctggcg ttgctcagca
      781 ggttgaacag gatctgcctg atcctcactc cgtccgccac cacggtgagc gggtcgttgt
      841 ccagccggag gatgcattcc tgctgcttgg cggtgcggag gctcgcggta tcggccaccg
      901 ccccggtgat cgcttcccga aggtcggtgg tggcgaggga gagcgacatc cgcccggcct
      961 cgactttgga gaggtcgagc acgctgttga tgagggagag gagatgcttg ccgttccgga
     1021 gcacggcctc agtgaactcg cgttgctgcg gactgatgtc gccggcgccg ccctccagca
     1081 ggagatcgga gaatccgatg atggcgttga gcggggtccg cagctcgtgc gacatgttgg
     1141 tgagaaactc gctcttgagc tgggtggccc gctgcagctc ctgattcttg gtgcgcagcg
     1201 cgagctcgtt gcgcctgctc tccgccagca ccgaggcccg gtccaagccc accacaacct
     1261 ggtcgcccag gatctgcagc aggttgagat cgtgctctcc gaacggccgg ccgtcgcgat
     1321 gattgtagac gctaacggtt ccgaccacga cgcccgccga gcgaagcggc acgatggcgg
     1381 cggtcttaag cggcacggtg aaagtgggcg gctgatagct gcgcgggtcg gcgttcatgt
     1441 catccgacac cacggccgca tcctgggtga cggcccagcc gagcagcgac ccgtccatcg
     1501 gcaccagcat gccgatcgcc tgctccagcg caccggtggc ggctttcacc ttgagaaagc
     1561 ggccttcctc caccaccagc gtcaccgccc cactctccgc ctcgatcagc tcggcggccg
     1621 ccttggccac c
//
LOCUS       DATADN010000058         3213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1213903, whole genome shotgun sequence.
ACCESSION   DATADN010000058 DATADN010000000
VERSION     DATADN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213903"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1150)
                     /locus_tag="VHH53_00760"
     CDS             complement(62..1150)
                     /locus_tag="VHH53_00760"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX2288746.1"
                     /translation="MTEVFRSGDHEQVVFCRDEPSGLQAIIAIHSTALGPALGGTRFH
                     PYPSEQDALADVLDLSRAMSYKNALAGLDHGGGKAVILADPSRDKTEALLRAYGRFVQ
                     SLGGRYVTACDVGTYVEDMDVVARESEFVTGRSLAHGGAGDSSILTAFGVFQGMRAAA
                     EHVWGEPTLSGRRIGIAGVGKVGRLLVDHVLADGAEVIVDDVNEQAIERVRAAHPEVA
                     VAPTTAALVAEPLDVYAPCALGWALDDPTVATLSAKIVCGGANNQLEHPGVEKLLEER
                     GILYAPDYVVNSGGVIQVADEIEGFDFERAKARVTRIFDTTREIFKLAEQDGVPPAVA
                     ADHLAERRMADVVRLRKILVDPRAALRR"
     gene            complement(1186..1308)
                     /locus_tag="VHH53_00765"
     CDS             complement(1186..1308)
                     /locus_tag="VHH53_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288747.1"
                     /translation="MLAQGDPTGLSSTTGQLVILFGLLAALVVLIRWWWQQRGR"
     gene            1351..1545
                     /locus_tag="VHH53_00770"
     CDS             1351..1545
                     /locus_tag="VHH53_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HEX2288748.1"
                     /translation="MRITHFRQRMDEEFGPVRAAALAHDHVFSELGGRTVDEALESGF
                     DTKQVWAVVCATFDVPEERR"
     gene            1804..2844
                     /gene="recA"
                     /locus_tag="VHH53_00775"
     CDS             1804..2844
                     /gene="recA"
                     /locus_tag="VHH53_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX2288749.1"
                     /translation="MAAAPDREKALEIALAQIDKQYGKGSVMRLGEEGRAPVEVIPTG
                     SIALDVALGIGGLPRGRVIEVYGPESSGKTTVALHAVANAQRAGGIAAFIDAEHALDP
                     DYAKSLGVDTDALLVSQPDTGEQALEIADMLIRSGALDVVVIDSVAALVPRAEIEGEM
                     GDAHVGLQARLMSQALRKITGALSNSGTTAIFINQLREKVGVFFGSPETTTGGKALKF
                     YASVRLDIRRIETLKDGSDAVGNRTRVKVVKNKCAPPFKQAEFDILYGHGISKEGSLI
                     DLGVEQGVVRKSGAWYTYEGDQLGQGKENARKFLGDNPDIAAEIEKRIKDKLGIGAKL
                     DADQTVPAPVDF"
     gene            2943..>3213
                     /locus_tag="VHH53_00780"
     CDS             2943..>3213
                     /locus_tag="VHH53_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX2288750.1"
                     /translation="MSPAAAAQRICLKLLTTRSRSRVELAEALCRRGIPEDVGEAVLD
                     RLGEVGLVNDAAFAESAVYSSHHHRGLGRRALSTELRRRGVPEEIV"
BASE COUNT          570 a         1010 c         1100 g          533 t
ORIGIN      
        1 ggagcgcgct cagcggagtt ggagacatcc cggttgccgc gtccagcctg ctgaatacaa
       61 gctatcgccg cagggcggcg cgggggtcga ccaggatttt gcgcagccgt accacatcag
      121 ccatccgtcg ctcggccagg tggtcggcgg ccacggcggg cggcacaccg tcctgctcag
      181 ccagcttgaa gatctcccgg gtggtgtcga agatccgggt aacccgggct ttggcccgct
      241 cgaaatcgaa gccctcgatc tcatcggcga cctgaatgac gccaccggag ttcaccacgt
      301 agtcgggcgc gtagaggatg ccccgttcct cgagcagctt ctcgacgccg ggatgttcca
      361 gctggttgtt tgcccctcca cagacgatct tggctgacag cgtggcaaca gtgggatcgt
      421 ccagcgccca accgagcgcg cacggcgcat acacgtcgag tggttcggcg accagcgccg
      481 cggtggtcgg cgcgacagcg acctccgggt gcgccgcacg gacccgttcg atcgcctgct
      541 cattgacatc atcgacgatg acttctgcac cgtcggcgag cacgtggtcg acgagcagcc
      601 tcccgacctt gccgaccccg gcgatgccta tccgacgtcc ggacaaggtc ggttcacccc
      661 atacgtgctc ggcggccgca cgcatgccct ggaagacgcc gaacgcggtg agaatcgagg
      721 aatcaccggc gccgccatgg gccaacgagc gtccggtgac gaactcggac tccctggcga
      781 cgacgtccat gtcctcgacg taggtgccga catcgcatgc ggtgacatag cgcccgccca
      841 gcgactgcac gaagcgcccg tatgcgcgca ggagcgcctc ggttttatcg cgggatgggt
      901 cggcgaggat gacggcctta ccaccgccgt ggtccagccc ggcgagtgcg ttcttgtagg
      961 acattgcgcg agacagatcc agcacgtcgg ccagcgcgtc ctgctcgctg gggtaagggt
     1021 ggaaccgagt gccgccgagc gctggcccca gcgcggtgga gtggatggcg atgatggcct
     1081 gcagccctga gggctcgtcg cggcagaaca cgacctgctc atggtcgccg gatcggaaca
     1141 cctcggtcac ggtggtgact cccttgctcg gtgcagtcgc gttgatcagc ggccgcgttg
     1201 ttgccaccac cagcggatga gcaccaccaa agcggcgagc aggccgaaaa ggatcaccag
     1261 ttgtccggtg gtggaggaca gtccggtcgg gtcgccctgg gcaagcaccc cttcacggta
     1321 aacccggagg cgggcaccta acctggcgga gtgcggataa cccatttccg gcagcggatg
     1381 gacgaggagt tcgggccggt gcgggcagcc gcgctggccc atgaccacgt gttcagcgaa
     1441 ctcggcggcc gtacggtgga cgaggcgctg gagtcggggt tcgacaccaa gcaagtgtgg
     1501 gccgtggtgt gtgccacatt cgacgtgccc gaagaacggc gctgatacac ccgctccttg
     1561 ccaccgcctg cgtgtccgtc tctcgtgctc ggtgtgtcgc ctcttggcgc gcgtccgccg
     1621 ccgttgggcg cgcggcttag catcccggcg tgtcacccac ggtgtcgaac acgtgttcgc
     1681 ctaatctgtt gtccacagcc ggtctcctgt ccacaggtcc ggcgtgtgtc gccgcgaagt
     1741 gtcagtccca tcgcttagcg tcggcgccga cccagaacaa ctgacagcga ggtggaccgt
     1801 cagatggcag cagcaccgga tcgggagaag gcgttagaga tcgccttggc ccagatcgac
     1861 aagcagtacg gcaagggctc ggtgatgcgc ctcggcgagg agggccgcgc acccgtcgag
     1921 gtgatcccca ccggttccat cgcattggac gtggcgctcg gcatcggcgg cctgcctcgc
     1981 ggcagggtga tcgaagtgta tggcccggag tcctccggca agacgacggt tgcgttgcac
     2041 gcagtggcca acgctcagcg ggccggcggg atagccgcgt tcatcgacgc tgagcatgcg
     2101 ctggatcccg actacgcgaa gtcactcgga gtggacaccg acgccctgct ggtctcccag
     2161 cccgacaccg gggaacaggc gctggagatc gcggacatgc tgatccgctc cggcgcgctg
     2221 gacgtcgtgg ttatcgactc ggtggccgcg ctggtgcccc gtgcagagat cgagggtgag
     2281 atgggtgacg cgcatgtcgg cttgcaggcc cggctgatga gccaggcgct gcgcaagatc
     2341 accggtgcgc tgagcaattc gggcacaact gcgatcttca tcaatcagct ccgggagaag
     2401 gtcggagtct ttttcggctc ccccgagacc actacaggtg gcaaggcgct gaagttctac
     2461 gcctcggtgc ggctggacat ccgccggatt gagacgctga aggacggctc agacgccgtc
     2521 ggcaaccgga cccgcgtcaa ggtggtaaaa aataaatgcg ccccgccatt caaacaagcg
     2581 gagttcgaca ttctgtatgg tcacgggatc agcaaggaag gctcactgat tgacctaggc
     2641 gtggagcagg gagtcgttcg caagtcaggt gcctggtaca cctacgaggg ggatcagctc
     2701 ggtcagggca aggagaacgc ccgcaagttc ctcggcgaca accccgacat cgctgctgag
     2761 atcgagaagc ggatcaagga caagctcggc attggggcga agctagacgc tgatcagacc
     2821 gtgccagctc cggtggactt ctgatgctgg cgacttgcac cgaggtgcca cgggctgcga
     2881 tgggtagacg tagtccagga gccaacgggt cagccaggga ggattccccg gctgccacgg
     2941 ttatgagtcc ggcggcagcg gcacagcgga tctgcctgaa gctcctgaca acgcggtcgc
     3001 gcagccgagt tgagctcgcc gaggccctct gtagacgcgg aattccggag gatgtcgggg
     3061 aagcggtcct tgaccgtctg ggcgaggttg gcctggtgaa tgacgccgcc ttcgccgagt
     3121 cggccgtgta ctcgagccac caccaccgcg gtctgggcag gcgggcattg agcactgagc
     3181 tccggcgcag gggcgtgccg gaggagatcg tca
//
LOCUS       DATADN010000059         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1214027, whole genome shotgun sequence.
ACCESSION   DATADN010000059 DATADN010000000
VERSION     DATADN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214027"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VHH53_00785"
     CDS             <1..328
                     /locus_tag="VHH53_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEX2288751.1"
                     /translation="CVFTGQGRIAITAKGAPVVLNVDQPTYADPQAAIAWSAALQTGF
                     HRADQIGLGTLLGRSTGEAFTMSFAGQGFVIVQPSEELPASMISGTGSGDQSGSGGLL
                     GGLMGR"
     gene            334..891
                     /gene="idi"
                     /locus_tag="VHH53_00790"
     CDS             334..891
                     /gene="idi"
                     /locus_tag="VHH53_00790"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX2288752.1"
                     /translation="MHPDDAPVVLLDEDRRPIGQLPKSQVHHATTPLHLAFSCYVFDG
                     SGRLLMTRRATSKRTWPGVWTNTCCGHPLPDESSAQAAARRLREELGVQMVRSEIALP
                     DFRYRAVASDGVVENEICPVLVASIHGSPQPNPAEVLEYRWAPWPDVVALATVAPWAI
                     SPWAVLQIPLQAALNFPHSAGSAGL"
     gene            complement(886..2169)
                     /locus_tag="VHH53_00795"
     CDS             complement(886..2169)
                     /locus_tag="VHH53_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2288753.1"
                     /translation="MLGTRGFGRLLSSRFAAQWGDGVFQAGLGGAVLFNPERQADPLA
                     VAAGLAVLLLPYSLVGPFAGALLDRWDRRRILVIANLLRVVPIVLVALAVGAGVAGAP
                     LYLGALLVTGVSRFVLVGLSAGLPHVVPRRHLVEANALAATVGAAIAVFGGICAVGLR
                     ALTGSGNTGSAWVTAAAVMGSLVAAATTARLRRGQLGPDSTDEPSETARAIARGLRDG
                     LTAAARVPSVAAGLVALAAHRLSFGIMTLVSLLLYRNSFTSDGLLRAGLAGVFQLLVA
                     GAVGLLLAAVLTPLLVARFGRGRTVRGALLAAGLLIVTLGLPMIMPTMLLAAFALSAA
                     GQVVKLSLDATVQSDVGDAVRGRVFALYDAVFNVGYVLAVALAATIVPADGRAPQLLL
                     LAAAAYLLAAAVYRLIDRGSTSAHSAGSAGLSDTS"
     gene            2299..>2850
                     /locus_tag="VHH53_00800"
     CDS             2299..>2850
                     /locus_tag="VHH53_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX2288754.1"
                     /translation="MSSTWQHRRNVRDDVEVDEVEPGSALVAAPALRDPNFCRTVVLV
                     IDHRVSGTLGVVLNRPSDVPVGEVLPLWGPHATLPQALYVGGPMEQRAALCVAALPAG
                     LDAAQTKGMIKVRGSLALVSLDADPDVMAPRLRGLRVFAGYAGWDQGQLAAEIERGDW
                     VVVPALPDDVLAPPGTDLWGRALR"
BASE COUNT          464 a          982 c         1002 g          402 t
ORIGIN      
        1 ctgcgtgttc accgggcagg gccggatcgc gatcaccgcc aagggtgcac cggtggtgct
       61 caatgtcgat cagcccacct acgccgaccc gcaggcggcg atcgcctggt ccgcggcgct
      121 gcagaccggg tttcaccggg ccgaccagat aggcctgggc acgctgctgg gtcgcagcac
      181 tggagaggcg ttcacgatga gcttcgccgg ccagggcttc gtcatcgtgc aaccctcgga
      241 agaactgccg gcgagcatga taagtggcac cggaagcggg gatcaatccg ggtccggcgg
      301 gctgctcggt gggctgatgg gccgctgaca gctgtgcatc ctgacgacgc tccggtcgtc
      361 ctgctcgacg aggatcgccg tcccatcggg cagctgccca agtcgcaggt ccatcacgcc
      421 acgacgccgc tgcatctggc gttctcctgc tatgtcttcg acggttccgg ccggctgctg
      481 atgacccgcc gtgccaccag caagcggacc tggccgggag tgtggaccaa cacctgctgc
      541 gggcacccgc tgcctgatga atcctccgca caggcggcag cgcggcgact gcgcgaggag
      601 ttgggcgtgc agatggtgcg ctcggagatc gcgctgcctg atttccggta ccgggcggtg
      661 gcgtccgacg gcgtcgtgga gaacgagatc tgcccggttc tggtggccag catccacgga
      721 tcgccgcagc ccaacccagc cgaggtgctc gagtaccggt gggccccctg gcccgatgtg
      781 gtggctcttg caacggtcgc cccctgggcg atcagcccgt gggccgtcct ccaaatcccg
      841 ctccaggccg ctctgaactt tccgcattca gcaggctcag ccgggctatg aggtgtccga
      901 taaccccgct gagcccgctg aatgcgcaga agtcgagccg cggtcgatga ggcggtacac
      961 cgcggcggcg aggagatagg cggcggcggc gagcagcagc agctgggggg cgcggccgtc
     1021 ggccggcacg atagtcgcgg ccagcgcgac cgccagcacg tagccgacgt tgaagaccgc
     1081 gtcatagagc gcgaagaccc ggccgcgcac cgcgtctccg acgtcgcttt gcaccgtggc
     1141 gtccaggctc agcttcacca cttgcccggc cgcggacagg gcgaacgctg ccagcagcat
     1201 cgtgggcatg atcatcggta ggcccagtgt gacgatcagt aacccggcgg ccagcagcgc
     1261 gccccggacc gtgcggcccc ggccgaacct ggccaccagc agcggggtga gcaccgcggc
     1321 gagtagcaac ccgaccgccc cggccaccag cagctggaag accccggcca gtccggcccg
     1381 aagtagcccg tcggaggtga aggagttgcg gtagagcagc agcgatacca gggtcatgat
     1441 gccgaatgag agccggtgtg cggccaatgc caccaggcca gcggcgacgc tgggtacccg
     1501 ggcggccgcg gtcaggccgt cgcgcaaccc tctggctatc gcgcgggctg tctcgctggg
     1561 ctcgtcggtg gagtccggtc cgagttggcc gcggcgcagc cgagcggtgg tcgcggcagc
     1621 gaccagcgag cccatcaccg cagccgccgt cacccaggct gatccggtgt tgcccgatcc
     1681 ggtcagggcg cgcagcccga ccgcgcagat cccgccgaag acagcgattg ccgcgccgac
     1741 ggtggcggcc agcgcattgg cctctaccag atgcctgcgt ggcaccacat ggggcaatcc
     1801 cgcggacagc cccaccagta cgaaccggct cacgccggtg accaggagcg ctccgaggta
     1861 cagcggcgcc ccggccaccc cagccccgac tgccagggcc accagcacaa tcggcacgac
     1921 gcgcagcaag ttcgcgatga ccaggatccg ccgacggtcc cagcggtcca gtagtgcccc
     1981 agcgaacggt cccaccaggg agtacggcag cagcagcacg gcgagcccgg ccgctacggc
     2041 gagcgggtcg gcttggcgct ccgggttgaa cagcacggcg ccgcccaatc cggcctggaa
     2101 gaccccgtcg ccccactgcg cggcgaaccg ggagctcagc agccggccga acccgcgggt
     2161 gcccagcaat cgccgaactc ctaatcggtc gggccggctg tcgagggcaa cggcttggga
     2221 cgacatcacg agcacgcagg ttagcggtgt catgtgggtg tggcgtcagg aaaatcataa
     2281 gtgtgcaccc aatcaagtat gtccagcact tggcagcacc ggcgcaatgt gcgagacgat
     2341 gtcgaggtgg acgaggtgga accgggctcg gcgctggtcg cggctcccgc gctgcgagat
     2401 cccaattttt gtcgcaccgt cgtgctggtc atcgaccacc gggtctcagg cacgctgggt
     2461 gtcgtgctca accggcccag tgatgtgccg gtgggcgagg tgctgccgct gtggggcccg
     2521 cacgccacgc tgccgcaggc gctgtacgtc ggcggtccca tggaacagcg cgcggcactg
     2581 tgcgtggccg cactgcccgc cggactggac gccgcgcaga ccaagggcat gatcaaagta
     2641 cggggatcgc tggccctggt cagcctcgat gccgatccgg acgtgatggc accccggctg
     2701 cgcgggctgc gagtgttcgc cggatacgcc ggatgggacc aaggccagct tgctgctgag
     2761 atcgagcgcg gtgactgggt cgtggtgccc gcactgcccg acgatgtgct cgccccgccg
     2821 ggcaccgatc tatggggccg cgcgctacgc
//
LOCUS       DATADN010000060         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_761794, whole genome shotgun sequence.
ACCESSION   DATADN010000060 DATADN010000000
VERSION     DATADN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761794"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VHH53_00805"
     CDS             <1..1321
                     /locus_tag="VHH53_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2288755.1"
                     /translation="TEMFHSRWLRLATALVSGACLALAGCSGDSDSGNGITKITFWDD
                     NGGPARTPVWEHIIAEFERANPTIDVEYVGVPIAQVQQKYDTAIAGGGLPDVGGVTTA
                     MLANLVPQKALDAVDDRLGASSINGKLNEQVLSTVRSTVPDGKLYMVPMSTNMGVFWY
                     RTDWFSQAGVQPPQTWDAFFAAVDKLTDTGQNRYGFTIRGGAGSIAQMLEVIYGQSGI
                     TEMFDSSGKSTVNDPRNVAALERLAALYKKNTPEADLTNDYAKMVAQFGGGTIGIMQH
                     NLGSYNDHVKAFGKDKVAAIAVPKSATGTRTVTSHPVSGLGVFASGQNKDAAFKFAEF
                     AAGKAMNSYWGEKTGQLPTNVEVVGESWLAGQQHIAEATKVLNDPSTKLVQLPYYLPE
                     FNAITKTDTEPLFQKVLLGEMSAKDFLDQFAGKFTEAQASYKQRNGG"
     gene            1338..>1605
                     /locus_tag="VHH53_00810"
     CDS             1338..>1605
                     /locus_tag="VHH53_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288756.1"
                     /translation="MKHFSLLVVAAAVAVAVAVPAAGATPAGHDRVPLGRQVLPANDG
                     WAAEGAGTSGGAAAPPENVHFVDSRAEFVTALAAPSPKIIYVHGV"
BASE COUNT          283 a          529 c          558 g          235 t
ORIGIN      
        1 gacggaaatg ttccactcgc gctggcttcg gctggccacc gcgctcgtct cgggcgcctg
       61 cctggcactg gccgggtgca gcggcgactc ggactccggc aacggcatca ccaagatcac
      121 cttctgggac gacaacggcg gtcccgcgcg caccccggtg tgggagcaca tcatcgccga
      181 gttcgagcgg gccaacccca cgatcgacgt ggagtacgtc ggtgtcccga ttgcccaggt
      241 gcagcagaag tacgacaccg cgatcgccgg cggtggcctg cccgacgtgg gcggcgtgac
      301 caccgcgatg ctggccaacc tggtgccgca gaaggcgttg gacgcggtgg atgaccggct
      361 cggcgccagc tcgatcaacg gcaagctcaa cgagcaggtg ctctccaccg tgcgctcgac
      421 ggtgccggac ggcaagctct acatggtgcc catgtccacc aacatgggcg tgttctggta
      481 ccgcaccgac tggttcagcc aggccggcgt gcagccgccg cagacctggg acgcgttctt
      541 cgccgcggtg gacaagctca ctgacaccgg ccagaaccgg tacggcttca ccatccgtgg
      601 tggtgccggc tcgatcgccc agatgctcga ggtgatctac ggccagtccg ggatcaccga
      661 gatgttcgac tcgtccggca agtccacggt caacgatccg cgcaacgtgg cggcgctgga
      721 gcggctcgcc gcgctgtaca agaagaacac gccggaagcc gacctcacca atgactacgc
      781 caagatggtc gcccagttcg gcggcgggac catcgggatc atgcagcaca acctgggctc
      841 ctacaacgac cacgtgaagg cgttcggcaa ggacaaggtc gccgccatcg cggtgccgaa
      901 atccgcgacc ggtacgcgga ccgtgacgtc gcacccggtg tccgggctgg gcgtcttcgc
      961 cagcgggcag aacaaggatg cggcattcaa gttcgctgag ttcgccgccg gcaaggcgat
     1021 gaacagttac tggggggaga agaccggcca gctgcccacc aacgtggagg tggtcgggga
     1081 gagctggctg gccgggcagc agcacatcgc cgaggccacc aaggtgctca acgacccgag
     1141 cacgaagctg gtgcagctgc cgtactacct gccggagttc aacgcgatca cgaagaccga
     1201 caccgagccg ctgttccaga aggtgctgct cggcgagatg tcggcgaagg acttcctgga
     1261 ccagttcgcg ggcaagttca ccgaggcgca ggcgagctac aagcagcgca acggcggctg
     1321 aggcggaggt tcctgccatg aagcactttt ccctgctggt agtcgctgcc gcggtggccg
     1381 tggccgttgc ggtacccgcg gcgggggcca cccccgccgg gcacgatcgg gtgccgctcg
     1441 ggcggcaggt gctgccggcc aacgacggtt gggcggccga gggtgccggc acctccggcg
     1501 gtgcggcggc accgccggag aacgtccatt tcgtggacag ccgcgcggag ttcgtcaccg
     1561 cgctggcggc gccgtcgccg aagatcattt acgtgcacgg ggtgg
//
LOCUS       DATADN010000061         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_309656, whole genome shotgun sequence.
ACCESSION   DATADN010000061 DATADN010000000
VERSION     DATADN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309656"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VHH53_00815"
     CDS             complement(<1..459)
                     /locus_tag="VHH53_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2288757.1"
                     /translation="MRTLVVAGHGMVGHRMVEALIGRDVARQWRIVVLAEEGRHAYDR
                     VALSSYVDSWDAQTLALPVVEDDRVELRLGDPCVAIDRRKRAVRTASGHRVAYDALVL
                     ATGSAPFVPPVPGHDVPGCYVYRTPEDLDAIRAAAQRAMQTTAAGRRSGVV"
     gene            complement(600..1985)
                     /locus_tag="VHH53_00820"
     CDS             complement(600..1985)
                     /locus_tag="VHH53_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEX2288758.1"
                     /translation="MSSTEMTAPAKPAHGGRWIDRWEPEDPVFWEQTGKKVANRNLWF
                     SILAEHIGFSMWTVWSVLVLFMGPAYGVDPAGKFFLVAFPALIGCLVRIPYTMAPAKF
                     GGRNWTIVSVLLLLIPTGLAIVVMEPGTSYTTFLLVAALGGLGGGNFASSMVNINAFF
                     PEGKKGWALGLNAGGGNIGVPAVQILGLLIIVTLGITHPRVVLAIYIPLILLAALGAV
                     KYMDNITSMRNDTGAMREAVRDSQTWVMSFLYIGTFGSFIGYSFAFGLVLQTQFGRTP
                     LQAASVVFIGPLIGSLIRPVGGWMADRFGGAKVTLVTFFGMAAATGLVLLASGQQSLP
                     VFTAGFIALFVLSGIGNGSTYKMIPSIFVGKAKMAMVVGETKEVALLNARRLAGAVIG
                     IAGAVGALGGVLINVAFRQSFLTTKSGDSAFWFFLAFYAVCVVVTYAVYLRRAPATEP
                     KHARRLAYAGV"
BASE COUNT          456 a          729 c          731 g          360 t
ORIGIN      
        1 caccaccccg gagcgccggc ccgccgctgt cgtctgcatt gcacgctgcg ctgccgctcg
       61 gatggcgtcg aggtcctccg gggtgcggta gacgtaacaa ccgggtacgt catggccggg
      121 aaccggaggc acaaaaggtg ccgatccagt ggccagcact agcgcgtcgt aggcgacccg
      181 gtgcccggac gcggtgcgga ctgcgcgctt gcgccgatcg atagccacgc acgggtcacc
      241 tagccgcagc tccacccggt cgtcctcgac cacgggcagc gccagcgtct gagcatccca
      301 cgagtccaca taggatgaca gggcgacccg gtcgtaggcg tgcctgccct cctcggcaag
      361 gaccacgatc cgccactgcc gcgcaacgtc gcggccgatc aaggcctcca ccatgcggtg
      421 cccgaccatc ccatggcctg cgacgacaag agtgcgcatc gggtggtcct tccttgctat
      481 gcagcagcgg actggtgaac tgcccggtaa cagaacagct caccagccgt ctaaatatct
      541 ttggtcatgg ttgacatcgt gagcgctctg cgtgaacgca gtgtttccgc actgtttcgt
      601 cacaccccgg cataggccag ccgccgcgca tgtttgggct ccgtggctgg tgcccggcgc
      661 aggtataccg cataggtgac aacgacacac accgcgtaga aggcgaggaa gaaccagaac
      721 gcggagtccc cggacttagt ggtgaggaat gactgccgga aggcaacatt gatgaggacc
      781 ccgcccaggg caccgaccgc accggcgatg ccgatcactg cgccggcgag tctgcgggcg
      841 ttgagcagcg cgacttcctt ggtctcacca actaccatcg ccatcttggc cttacccacg
      901 aagatcgacg ggatcatctt gtaggtggag ccgttaccaa tgcctgacag gacgaacagc
      961 gcaatgaagc cggctgtgaa caccggcaag gactgctgcc cagaagccag gagcaccagg
     1021 ccggtggcgg cggccatacc gaagaaagtg acgagggtga ccttggcgcc gccgaaccgg
     1081 tccgccatcc agcctccgac cggacgaatc agcgaaccga tcagcgggcc gatgaagacg
     1141 accgatgcgg cctgcagcgg ggtacggccg aactgggttt gtaataccag gccgaaggcg
     1201 aagctgtagc cgatgaatga accgaaggtg ccaatgtaga ggaaagacat cacccaagtc
     1261 tgcgaatcac ggaccgcttc gcgcatcgca ccggtgtcgt tacgcatcga cgtgatgttg
     1321 tccatgtatt tcacggcgcc cagtgcagcg agcagaatca gcgggatgta gatggccagc
     1381 actacgcgcg ggtgggtgat gccgagggtc acgatgatca gcaggccgag gatctgcact
     1441 gcgggcaccc cgatgttccc cccgccggcg ttgagtccga gcgcccagcc cttcttgccc
     1501 tccggaaaga aggcgttgat gttgaccatg gaggaggcga agttgccgcc gcccaggcca
     1561 ccaagtgccg cgaccagtag gaacgtggtg taggaagtgc ccggctccat caccacgatg
     1621 gccaggcccg tcgggatcag cagcaacagc acgctgacga tggtccagtt ccgcccgccg
     1681 aacttggccg gcgccatggt gtagggaatc cggaccagac agccgatcaa tgcggggaac
     1741 gccaccagga agaacttgcc ggccgggtcg acgccatacg ccgggcccat gaacagcacg
     1801 aggactgacc agaccgtcca catcgagaac ccgatgtgct cggcgaggat cgagaaccac
     1861 aggttccgat tcgccacctt cttgccggtc tgctcccaga agacggggtc ttccggctcc
     1921 cagcggtcga tccatcgacc cccgtgagcc ggcttggccg gcgccgtcat ctcggtcgag
     1981 ctcatcgcgc ctcctgcaga gctggtgggt acgaggagtg acgttaaatg ccggttatgt
     2041 tcggagtgtt gctgcttgtt gcagcgccga gaactcccgt tcacatggcg gggcggccgt
     2101 ggcgtgaggc cgccgtaggc gcggtgttcg gcgacccggt gcggaatggg gatgccgaaa
     2161 cctggatgcg ttgtggggac ctgcgcgtta ccgtggcgac atgatctgct gaggtccccc
     2221 acgcatccaa ctgaggagac ctgatggcac agcaacccga accccaggac aaaccc
//
LOCUS       DATADN010000062         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_460625, whole genome shotgun sequence.
ACCESSION   DATADN010000062 DATADN010000000
VERSION     DATADN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460625"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..926
                     /locus_tag="VHH53_00825"
     CDS             138..926
                     /locus_tag="VHH53_00825"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX2288759.1"
                     /translation="MPIQPRYTGPPSYPMPPRWGFSRLGWRWPAPEIGPAVSAVEQMR
                     GLAATAAPLLGLTALLMLATAGAEIWRYALLLDSRTDAVPAGPLHASDALVITGGVIS
                     LLAGILAGAVTVGWLIRACTVAAREAGVTPARRPWQLVAGVLVPGVNLLVPGAVLAEL
                     EHAALGRDPDHRPRPSRLVIGWWAIWVTGLVMVAITMLWNLRDGVQARADGVLLHAAT
                     DLVAAAVAIATIVVVRRLTRLLSPAKLIAARRMVVVRVSDGTRS"
     gene            981..1934
                     /locus_tag="VHH53_00830"
     CDS             981..1934
                     /locus_tag="VHH53_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5926 family protein"
                     /protein_id="HEX2288760.1"
                     /translation="MSKRSATKTRRSTNGEYNPRQSCPCGSGKRYKACHGDPGGAQDV
                     IVSRPFAGLAAECQLVALREFVPSATAPLPLAQPTGRDIAMATVLPTAAAAIVRPDGA
                     ALVGLQVLTRSGDLSRDLGRAVTWALTAEPGSVLPKVSTTAEGEQIRLQDLFVPDMPL
                     DVTMYRDFAWWIPGDEPPSGEAAKSIQRANSAIMPTEAVTGQGVDSAYWVDAGEKAHL
                     RWVRPEPEEQLLTALARLAARDELSLGDGSRYAGSFRAHGVLVPVWDLDRERHPREWV
                     APAEAFAQRLAQALDSLDGTPLTDAERRSARGLRGRQITLR"
BASE COUNT          295 a          705 c          756 g          334 t
ORIGIN      
        1 gcacgggtca ccgcttatct cgcgcaggcg gggtgggacg cggtgaacat cgacgggggg
       61 atgcgggcct gggcggctgc cgggcgcccg atggtggccg agtcgagcat ggcgccgagg
      121 gtgctgtagg accggcgatg cccatccagc cgcgctacac cgggcccccg tcctatccca
      181 tgccaccgcg ctggggtttc tcccggctgg gctggcgatg gccggcaccg gagatcggtc
      241 ccgcggtgag cgccgtggaa cagatgcgtg ggctggccgc tacggcggcc ccactgcttg
      301 ggctgaccgc cctgctgatg ctggccactg ccggtgccga gatatggcgt tacgcattgc
      361 tgctggacag ccgtaccgac gcggtacctg ccggaccgct gcacgccagc gacgcgttgg
      421 tgatcactgg tggagtgatc tcactgcttg ccgggattct ggcgggcgcg gtgacggtgg
      481 gatggctgat ccgggcctgc acggtcgcgg cccgggaagc cggggtcaca ccggcccggc
      541 gaccctggca gctagtcgcc ggcgtcctgg tgccgggggt gaacctgctg gtgccgggcg
      601 cggtgctcgc ggagctggag cacgcggcac tgggtaggga tccagaccac cgaccgcgcc
      661 cttcccggct cgtcatcgga tggtgggcga tctgggtgac cggactggtc atggtggcta
      721 tcaccatgct gtggaacctg cgcgacggag tgcaggcccg cgccgacggc gtgctgctgc
      781 acgcggcaac cgacttggta gccgcggctg tcgcgatcgc cacgatcgtt gtggttcgca
      841 ggctcacccg gttgctcagc ccggcgaaac tgatcgccgc ccgccggatg gtggtagtgc
      901 gggtgtctga tggcacccgg tcgtgagtgg cattcggtgt gggaacactg ttacgcactc
      961 acgaccgcta acctgcaccg gtgtccaaac gcagcgctac aaagacccgt cgttccacta
     1021 acggggagta caacccccgc cagtcctgcc cctgcggctc cggcaagagg tacaaggcct
     1081 gccacggtga cccgggcggc gcccaggacg tcatcgtcag ccgtcccttc gcggggctag
     1141 ccgcggagtg ccagctcgtc gcactgcgcg agttcgtgcc gtcggcaacc gcgcccttac
     1201 cgctggcgca gcccaccggg cgagacatcg cgatggctac cgtgctgccg acggccgccg
     1261 cggcgatagt gcggcccgac ggggcggcgc tggttggcct gcaagtgctg acccgctccg
     1321 gcgatctgtc ccgggacctt ggccgggcgg tgacctgggc gctgaccgcg gagcccggct
     1381 cggtgcttcc caaggtcagc accaccgccg agggcgagca gatccggctg caggatctat
     1441 tcgtccccga tatgcccctt gacgtcacca tgtaccgcga cttcgcctgg tggatccccg
     1501 gtgatgaacc cccgtccggc gaggcggcaa agtcgataca gcgggccaac tccgcgatca
     1561 tgccgactga agcggtcacc gggcagggtg tcgattccgc ctactgggtc gacgccgggg
     1621 agaaggcgca cctgcggtgg gtccgccccg aaccggagga gcagctactc accgcgctgg
     1681 cccggctggc ggcccgtgac gagctcagcc tcggcgacgg ctcgcgctat gcaggctcgt
     1741 tccgtgcgca cggtgtgttg gtgccggtct gggacctcga ccgtgagcga cacccccggg
     1801 agtgggtcgc ccctgcggag gctttcgcgc agcggttggc gcaagcgctg gactcactgg
     1861 acggcacgcc gctcaccgac gcggaacgcc gctctgcgcg tggtctccgc ggccgccaga
     1921 tcaccctgcg ctgaggcgca ccccttgctc tccctgcatt cagcgggctc agcgggattt
     1981 cacacgtcga aataatcccg ctgagcctgc tgaatgcggg ggttgcctta gggcagccag
     2041 gtgaggcggt cgcggaggag ggcgtagccg acgaaggcga caacgtcgat
//
LOCUS       DATADN010000063         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1214528, whole genome shotgun sequence.
ACCESSION   DATADN010000063 DATADN010000000
VERSION     DATADN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214528"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..591)
                     /locus_tag="VHH53_00835"
     CDS             complement(202..591)
                     /locus_tag="VHH53_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2288761.1"
                     /translation="MPPRTGRPWWLWLPTVSMAAFMTALVECAHAVGAGQGKPILLVL
                     DRAGWHVSPQVQVPAGIHLHFLPPYSPELQPAERLWPLTNEALANRHFRDADELQTVQ
                     AHRCLPLQARPEIIEAHTRFHWWPQTV"
     gene            complement(1285..1461)
                     /locus_tag="VHH53_00840"
                     /pseudo
     CDS             complement(1285..1461)
                     /locus_tag="VHH53_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          283 a          483 c          538 g          325 t
ORIGIN      
        1 gaccgtgtgc gcctgtcgag agtgcacgtg tttaacgtgt gagatgataa ggcacactgg
       61 tgacgaccac acccctcctg ttggatctgc tcaatacagt gcggcaatcg aagatccccc
      121 accgaagatc tcgcgcgcta atcgcgaaac cacagtctct cctcctttgg gagtaatacc
      181 aaatcctgtt gatttctgtg ttcatactgt ttgaggccac cagtggaaac gtgtatgggc
      241 ttcgatgatt tccggtctcg cttgcagcgg caggcagcgg tgggcctgga ccgtctggag
      301 ttcgtccgcg tcacgaaaat ggcgattggc cagtgcttcg ttggtcagcg gccacagtcg
      361 ttcggcaggc tgcagctcgg gtgagtacgg gggcaggaag tgcagatgga tgcctgcggg
      421 cacctgcacc tgcgggctca cgtgccagcc ggcgcgatcg agtacaagga ggatcggttt
      481 gccttgcccc gcgccgacgg catgggcaca ctcgacgagg gcggtcataa atgcggccat
      541 ggagacggtc gggagccata gccaccaggg ccgacccgtc cgtggcggca caacagcgta
      601 gaggtagcac cactgctagc gctgttgcac caccgccgcc gggcgctgcc cgcgcgggct
      661 ccacacgcgc cgccggatgg gtttacgccc gatgcggtgt tggtcggtcg cccaccacgc
      721 cactccctgc tgggggtgcg cttgctgtag ggcgcgcact acgatcggga ggtgtttttg
      781 aacgccatct gggccagggg gtccgccttg gcgtggcgcg ggcgcggcac tgtggaggtc
      841 cagcccaggc gccgcaacgc ctccacccgc gctggggatg cacgcgacgg cccagcgccg
      901 ccgtcatcca ggctgccgct ttgggccccg tccaggcgcc cccatcggct gggggctgct
      961 ggagggccac ggccagtgcg gcgcgctgag tggcggataa cagccctgtg gccccggggt
     1021 tgcggtggcg gcggtcctct agccccgcca gccctcgctg gttgtactgc cgtgcgctgg
     1081 tgcgcaccca gttgaccgtg tagccggtga ccgcggcgac gtgcgcggag ggcagccctc
     1141 gcgccagcag ccagacgacc tgccaatggc tgcgcgccac gggatctttc gcccggcgat
     1201 agtgggttcc cagttcgtca acaccgagat gggcttggat cgacagtcgt gttggcatgg
     1261 aatcattcta caccaggatt tggtatgatt ccattggcag cccgtgcgca gccggaaaat
     1321 gatgccattc aatacccggc gcagatccac gcggcgatgc cctttgggct tggcggggta
     1381 ccactcgttg agcatcggct cgatcaatgt ccagacgtca tcaggaactt cccagatggt
     1441 tggttgagcg gttgtgtcca tgataaccct ccctgtttgt acaacacagg ggagttgtcg
     1501 tcggaccgac tattttgatt gagatagttt gttattagtg ctgaatggcc ggccggaatt
     1561 ggggaagctg aaaattctca aagcggctga atttttgcga agaaacacaa agaactggtt
     1621 gcgagtttt
//
LOCUS       DATADN010000064         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_460905, whole genome shotgun sequence.
ACCESSION   DATADN010000064 DATADN010000000
VERSION     DATADN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460905"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1585
                     /locus_tag="VHH53_00845"
     CDS             23..1585
                     /locus_tag="VHH53_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX2288762.1"
                     /translation="MLRNTWRGLTSMRTALVLLFLLALAALPGALLPQYSLNPERVRT
                     YKAQYPTLAPILDRLGFFEVFASPWFAAIYLLLFVSLVGCVLPRSVEYARQLRRTPVA
                     TPRNLGRLPYHARGECASRPDEVLAAARRRLRGWRIAERDEPNGARTLSAERGYAREA
                     GNLVFHLALLGLLVSFAVGKLVGYEGQVIVLANGSQFCNSGPLAYDSFRPGLSVDGTA
                     LDPFCVKVDGFTPTYLNTGQPEEFRASIHYQGPDDLSTGSWHPYQLKVNDPLRMDGNR
                     VYLLGHGYAPQFTVTFPDGQQRSGVVQWRPVDIATFLSEGATKFDPPGVTDDAERRTR
                     QLAITGLFAPTAAFQGALLSSGFPDLLNPAVAIDVLKGDLGNNSGRGQSIFSIDQSMV
                     DSGRLNRVARGNLFPGEQLTLPDRTVVRFDGVTRFASLQVSYDPAQRWVLGFAIGVFG
                     GLALSLAVKRRRFWVRVSPAGPDGTVVELGGLARTEAAGYGEEFDRIAVDLLPPGSPQ
                     PTATAAVSKEGP"
     gene            1588..2550
                     /gene="ccsB"
                     /locus_tag="VHH53_00850"
     CDS             1588..2550
                     /gene="ccsB"
                     /locus_tag="VHH53_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEX2288763.1"
                     /translation="MPVNPVLASYSDLAYGTALVLYLFAMVLHAVGYAAGRVAVTGAG
                     APRVAGGGDGPVATLARPHDSPGRRPLRDTSERFGRCAVALTALAALVHGSSLVLRGA
                     AVGRAPWGNMYEFTSAFCVVAVFAWLVVLYRQRGGVALQRLGGFVLLPVVVLMFLGGT
                     VLYAQAAPLVPALNSYWIAIHVSAAAIASGILLVSGVASVLYLVREGAGSRITVRLPD
                     AATLDRLAYRTAVVALPIWTFAVITGAIWAEAAWGRYWGWDPKETCALVAWVVYAGYL
                     HARATAGWRGSPAAWINVVGFAVMLFNLFFINIVIAGFHSYAGI"
     gene            2537..>3352
                     /locus_tag="VHH53_00855"
     CDS             2537..>3352
                     /locus_tag="VHH53_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX2288764.1"
                     /translation="MPGSDTGPPAGRAPGGHRDGQAGLPAHDLSSAQLLRQTKRSPQS
                     GWRRVLFVASGRTINLGESPSELQRRELISRINQPLQGCYKIAMLSLKGGVGKTTITA
                     TLGSTFASLRGDRVIGVDANPDRGTLAQKIPLETTATVRHLLRDADRIRRYSDIRSYT
                     SQGPSRLEILASEQDPAVSQAYSEDDYRRTVNLLEHFYNIVLTDCGTGLMHSAMYGVL
                     GLADHLVIVSSGSIDGARSASATLDWLDAHGYRDLVSRSVAVINSVRRSSGNVD"
BASE COUNT          449 a         1172 c         1132 g          599 t
ORIGIN      
        1 ctcggcatac cccgtcaccg cggtgctgcg caacacctgg cgcgggctga ccagcatgcg
       61 caccgcgctg gtgctgctgt tcctgctggc gctggccgcg ctacccggcg ccctgttacc
      121 gcagtactcg ctgaaccccg aacgggtccg gacatacaag gcgcagtacc cgacgctggc
      181 cccgatactg gaccggctgg gcttcttcga ggtcttcgcc agcccctggt tcgccgcgat
      241 ctacctgctg ctgttcgtct ccctcgtggg ttgcgtgctg ccccgcagcg tcgagtacgc
      301 ccggcagctg cgccggaccc cggtggccac cccccgcaac ctgggccggc tgccatacca
      361 tgctcgcggc gaatgcgcgt cccgccccga tgaggtgctg gccgccgccc ggcgacggct
      421 acgcggctgg cggatcgccg aacgcgacga gcccaatggt gcccgcacgt tgtcggccga
      481 gcggggctat gcacgggagg ccggcaacct cgtcttccat ctcgccttgc tgggcctgct
      541 ggtgtccttc gcggtcggca agctggtcgg ttacgagggt caggtgatcg tgctggcgaa
      601 cgggtcgcag ttctgcaatt ccggcccgct ggcctatgac tcctttcgcc ccggcctgtc
      661 ggtcgacggc accgccctgg acccgttctg cgtcaaggtc gacggattca ccccgactta
      721 tctgaatacc gggcagccgg aggagttccg cgccagcatc cactatcagg gacccgacga
      781 cctgagcacc ggcagctggc atccgtatca actgaaggtc aacgacccac tgcggatgga
      841 cggcaaccgg gtctacctgc tcggtcacgg ttacgcgccg cagttcaccg tcaccttccc
      901 cgacgggcag cagcgcagcg gcgtggtgca gtggcgcccg gtggacatcg ccaccttcct
      961 ctccgagggc gccaccaagt tcgacccgcc tggggtgacc gacgacgccg agcggcgtac
     1021 ccgccagctc gcgatcaccg gactgttcgc gccgaccgcg gcgttccagg gcgcattgct
     1081 gtcctcgggg ttccccgacc tgctcaaccc ggccgtcgcg attgacgtgc tcaaggggga
     1141 tctgggcaac aactccggcc gcggccagtc catcttctcc atcgaccagt ccatggtcga
     1201 ctccgggcgg ctcaaccgag tggcccgggg caacctgttc cccggcgagc agctcaccct
     1261 gcccgatcgc acggtggtgc gcttcgacgg cgtgacccga ttcgcctcgc tgcaggtctc
     1321 ctacgaccca gcccagcgct gggtgctcgg tttcgcgatt ggagtcttcg gcggccttgc
     1381 gctgtcgctg gcggtgaagc ggcgccggtt ctgggtgcgc gtcagcccag ccgggccaga
     1441 cggtaccgtg gtcgagctgg gtgggctggc ccgcaccgag gccgccgggt acggggagga
     1501 gttcgaccgg atcgccgtcg acctgctgcc gcccgggtcc ccgcagccaa cggcgaccgc
     1561 ggcagtgtca aaggagggac cgtagcgatg ccagtcaatc cggttttggc gtcctacagt
     1621 gacctcgcgt acggcaccgc tctggtgctc tacctgttcg cgatggtgct gcacgccgtc
     1681 gggtatgcgg ccgggcgggt cgcggtgacc ggtgctggcg caccgcgggt ggccggtggg
     1741 ggagatggac cggtcgccac gttggcccgg ccgcatgatt ccccaggacg tcgcccgctg
     1801 cgggacacct ccgagcgatt cgggcgttgc gcggtggcgc tcaccgcgct cgctgcgctg
     1861 gtacacggta gctcgctggt actgcggggc gctgctgtcg ggcgggcgcc ctggggcaac
     1921 atgtacgagt tcacctcggc cttctgcgtc gtcgcagtgt tcgcttggct ggtggtgctg
     1981 taccggcagc ggggtggggt cgccctgcag cggctgggtg ggttcgtgct gctgcccgtg
     2041 gtggtgttga tgttcctggg cggcacggtg ctctacgccc aggccgcgcc gctggtgccg
     2101 gcgttgaact cgtactggat cgccattcat gtctccgcgg cggccatcgc atcgggcatc
     2161 ctgctggtct ccggcgtcgc cagcgtgctg tacctggtcc gggaaggggc tggatcccgg
     2221 atcaccgtcc ggttacccga cgccgccact ctggaccggc tggcttaccg caccgcggtg
     2281 gttgcgctgc cgatctggac cttcgccgtc atcaccgggg cgatctgggc cgaggccgct
     2341 tggggtcgct actggggttg ggatccgaag gagacgtgcg cgctggtcgc ctgggtggtc
     2401 tacgcggggt atttgcacgc cagggcgacc gctggatggc gcggttcacc ggcggcctgg
     2461 atcaatgtgg tgggctttgc ggtgatgctg ttcaacctgt tcttcatcaa catcgtcatc
     2521 gccgggttcc actcctatgc cgggatctga caccgggccg ccggcaggtc gggctcccgg
     2581 cggtcaccgc gacggccaag ctgggctccc cgcgcatgac ctgtcttccg cgcagctgct
     2641 tcggcagacc aaaagatccc cgcagtccgg ctggcggcgg gtgttgttcg tcgcctcagg
     2701 acgcaccatc aacctcgggg agagtccctc cgaactacag cgccgagaac tgatctcccg
     2761 gatcaaccag ccgctgcagg gctgctacaa gatcgcgatg ctgagcctca agggcggcgt
     2821 cggcaaaacg accatcaccg cgacgttggg ctccaccttc gcctcgttgc gcggtgatcg
     2881 ggtgatcggg gtggacgcca atcccgaccg cggcacgctg gcccagaaaa ttccgctgga
     2941 gacgaccgcc accgtgcggc atctactgcg cgacgccgac cggatccgtc gctactccga
     3001 catccgttcc tacacctcgc aaggccccag ccggttggag attctggcca gcgagcagga
     3061 tccggcggtc tcgcaggcct atagcgagga tgactaccgg cgcacggtga acctgctcga
     3121 gcacttctac aacatcgtgc tcaccgactg cggcaccggg ctgatgcact cggcgatgta
     3181 cggcgtgctc ggcctcgccg accatctcgt catcgtgtcg tccggctcga tcgacggggc
     3241 ccgcagcgcc tcggccaccc tggactggct cgacgcgcac ggctaccgcg acctggtgtc
     3301 gcgctcggtg gccgtcatca actccgtgcg ccggtcgtcg ggcaacgtcg ac
//
LOCUS       DATADN010000065         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_762262, whole genome shotgun sequence.
ACCESSION   DATADN010000065 DATADN010000000
VERSION     DATADN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762262"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VHH53_00860"
     CDS             <1..1428
                     /locus_tag="VHH53_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HEX2288765.1"
                     /translation="VWNWHNPLLITFTADGDAVFNPDYLTDVKQDAVDGNTRVTYTIN
                     PKATYNDGTPIDWRSFEAVYKTNSATNPAYIVNSTDGYDRIASVSRGVDDRQAVVTFR
                     GVNLWWQSLFNNLIHPNAVDPQVFNQGYIDNPHPEWGAGPYQIQNFDKRNGTITFVRN
                     PKWWGKTGKLDSRTFLTMESAASINAFKNGQLDATGVATKDRLAQVQGMSGIDIRKSI
                     EPALNYFTLNGESPILADPAVRKAVFEGIDRQQLQEIHFQGLNLTAQLPGSMVLLPFQ
                     DGYQDNFTKMITFNPEQAKQGLEAAGWVPGPDGVRAKDGKPLEYTYVNTGDDPTGKAI
                     ASATAAMLKNIGVRLNIRQVPASEFSTIVTGKRFDMFYSGVSQSDPYGIAYICQLYCS
                     DSTLIKSGVNSPALDAQLRAINQLPTPQEQYTKANEVETEAFKTYGVMPTINRPAIVA
                     VKQGLANYGAGRFFDATPETIGWQK"
     gene            1523..1948
                     /locus_tag="VHH53_00865"
     CDS             1523..1948
                     /locus_tag="VHH53_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288766.1"
                     /translation="MAQPGQFRGRVDEYYSSAGASVITADPAVIPVIWRGARRPMYFQ
                     NGGYMVPRRKSTGITVQGRYTNGAIAALVAPYGRGKVGLRGPHLEAPPEWYREANLSY
                     DLCRWHRVPTALQFSDVRRSQLRALVREPLTQRVAGHLW"
BASE COUNT          471 a          746 c          681 g          351 t
ORIGIN      
        1 gtgtggaact ggcacaaccc gctgctgatc accttcaccg ccgacggaga cgccgtcttc
       61 aaccccgact acctgaccga cgtcaagcag gacgcggtcg acggcaacac ccgcgtcacc
      121 tacacgatca acccgaaagc cacctacaac gacggcacgc cgatcgactg gcgctcgttc
      181 gaggccgtct acaagaccaa cagcgccacc aacccggcgt acattgtcaa ttccaccgac
      241 ggctacgacc ggatcgcctc ggtgagccgg ggtgtggacg accgccaggc agtggtcacc
      301 ttccgcggcg tgaacctgtg gtggcagagc ctgttcaaca acctgatcca ccccaacgcg
      361 gtggatccgc aggtgttcaa tcagggttac atcgacaacc cgcacccgga gtggggggcc
      421 ggcccgtacc agatccagaa cttcgacaag cgcaacggca ccatcacctt cgtccgcaac
      481 ccgaagtggt ggggcaagac gggcaaattg gactcgcgga cgttcctgac catggagtcc
      541 gccgcctcca tcaacgcgtt caagaacggc cagctcgatg cgacaggagt ggccaccaag
      601 gaccggttgg cccaggtgca gggcatgagc gggatcgaca tccgcaagag catcgagccg
      661 gcgctgaact acttcaccct caacggcgag agcccgatcc tcgccgaccc ggcggtgcgc
      721 aaggcggtgt tcgaggggat cgatcgccag cagttgcagg agattcactt ccagggcctg
      781 aacctgaccg cgcagctgcc cggttcgatg gtcttgctgc ccttccagga cggctaccag
      841 gacaatttca ccaaaatgat caccttcaac cccgagcagg ccaaacaagg ccttgaggcg
      901 gcgggctggg tgcctggccc ggacggcgtc cgggccaagg acggcaagcc gctggagtac
      961 acctacgtca acaccggcga cgatccgacc ggcaaggcca tcgccagtgc caccgccgcc
     1021 atgctcaaga acatcggggt gcggctgaac atccggcagg tcccggccag cgagttctcc
     1081 acgatcgtca ccggaaagcg gttcgacatg ttctactccg gggtctccca gtccgacccg
     1141 tacggcatcg cctacatctg ccagctctac tgctcggaca gcacgctcat caagtccggg
     1201 gtgaacagcc cggcgctgga cgcccagctg cgggcgatca accagctgcc cacacctcag
     1261 gagcagtaca cgaaggccaa cgaggtggag accgaggcgt tcaagaccta cggggtcatg
     1321 ccgacgatca accggccggc catcgtcgcc gtcaagcagg gcctggccaa ctacggcgct
     1381 gggcgcttct tcgatgccac gccggaaacc atcggctggc agaagtagcc tcagcgggta
     1441 cttgaggtga ggcgacaagc tcgcgatcaa gcatttcgtt cacgaaggcg cgcgttacct
     1501 gatgatctgc atgggtggct tcttggcgca accgggccag ttccggggca gggtcgatga
     1561 gtactactcg tcggcagggg catcggtaat caccgccgac cctgccgtca tcccggtgat
     1621 ctggcgcggc gcaaggcgcc cgatgtactt ccagaacggc ggctacatgg tgccgcgacg
     1681 caagtccacc gggatcaccg tgcaggggcg atacaccaat ggagcgatcg cggcgctggt
     1741 cgctccttac ggccgtggca aggtgggcct gcgcggtccg catctcgaag ctcctcccga
     1801 gtggtaccga gaagcgaacc tcagctatga cctgtgccga tggcaccgag ttcccacggc
     1861 gctgcagttc agcgacgtca ggcgcagcca gctgcgggct ttggtccgag aacccttgac
     1921 tcaacgagtc gctggtcacc tttggtaagg catcgccagc ggtaatgaca gcgattaccg
     1981 caaccaccag attcgtggcg cgtcgccggt ctgctgaagc agccgacgcc ctattgggcg
     2041 aacaacggcg atttcgaccg ctacaccgag tcattgacag ccaggcgctg ccgtgcaaaa
     2101 ggcgcgagat tgctagcgac tgattactcg ccgagcaacc gcatggacac tgtatgtcaa
     2161 aaagaataaa gtacgccggc acgcccagtc ggacgtacac cgtagctagg taccgggcgt
     2221 cggacaccta cgtccgtgtc accgcggac
//
LOCUS       DATADN010000066         3261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_159222, whole genome shotgun sequence.
ACCESSION   DATADN010000066 DATADN010000000
VERSION     DATADN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159222"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2589
                     /locus_tag="VHH53_00870"
     CDS             <1..2589
                     /locus_tag="VHH53_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2288767.1"
                     /translation="GPSVRRIRVRSLWARRPRVLLKSPSTVVVVVLTVIASAGLNGLA
                     SGQPGTVPLAAGLPPAAGLPPAPLQGKIGPQLAATRGPVAVFVELAQTPAVDVFHAQR
                     GAGRPATTAAQAASAAKSQVEQAADRALAMLRHHEAGVHELFRTTNAVPGLAVIVGGE
                     TIRRLAGSPDIRSVHKIVPKTAMNSSAVQLTRTLHAWQQTGWLGDGVRIGIIDDGIDY
                     THADFGGPGTPAAYTAIDRTMIKPSYFPTRKVVSGYDFAGDDYDGGVAGDPLGAVPRP
                     DPNPLSCGQHGNHVAGTAAGFGVNADGSTFRGDYQHLDPAALNALRIGPGTAPHALLY
                     ALKVFGCRGATGLTAKAMDWALDPNGDGDFSDRLDVVNLSLGSDYGAPDDPDSLFVRQ
                     LAANGVLPVFAAGNGGDLYDIGGAPGNTPEALTVASTRDSAVLRDGAEVAAVSGDQIV
                     GGQIVGGQYSQDFPSYDSLDRTAPVVRLSDPANADGCQPYSAADAAAVMGKHAWLEWD
                     DDNATRRCGSKVRADHALAAKAAGVLLSSAEEHFAASIAGNAGLPMFQLTGLDTAALR
                     PALQAGTLVVRLAGKLRTSVHTDDPRITDTVSEFSSRDVRGPAVKPDVAAPGDTIASA
                     LTGTGSDVLAISGTSMASPHVAGIAALVRQAHPDWNAAEVKAAVVNTAGADVHSRDGQ
                     QGPMAAPNRVGTGRVDAAAAVRTQVLAMVPDDPGQVSAGFGVVQATGPVTLSKKITLV
                     NKGATAARYAVAYQPITTMPGVDYHVSSRSVDIAPRGVAALTVTLRIDDPSALRRTAD
                     PTIEKTQLGAARQFVADASGRIIFSAADSGAGVPQDTVNPAGAGLCRCEARVRPVGTR
                     ESALPA"
     gene            2602..>3261
                     /locus_tag="VHH53_00875"
     CDS             2602..>3261
                     /locus_tag="VHH53_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HEX2288768.1"
                     /translation="MLPLSGRGVDQGSGDAAYRSLISVLQLQTESGRLGECPGIFVAA
                     CTPNGTARGGDIRYVGVTSTAPLAAARGRPKDALLAFGITTWEDWYNVGSNTIPFVNI
                     DTTGDGLPDFEVRGTKLTGTDVLVASTADLHAPPGSPPVQVLPVNGQFGDVDSGVFDS
                     NIIMLPVSLAALRIDPLAVTAPISYTTAVASHYASPVSVDEVIDSTVPALFDAVRPGL
                     WA"
BASE COUNT          510 a         1125 c         1135 g          491 t
ORIGIN      
        1 ggtccgtcag tgcgccgcat ccgggtacgc agcctgtggg ctcgccgccc ccgagtcctg
       61 ctgaagagtc cgtcgacggt cgtggttgtg gtgctcaccg tgatcgcctc ggcgggactc
      121 aacggcctgg ccagcggtca gcccggcacg gtgccgctcg cggccggctt gccgccggcg
      181 gcaggcctac caccggcacc gctgcaaggc aagatcggcc cacagctggc cgcgacgcgg
      241 gggccggtgg cggtctttgt cgaactcgcc cagactccgg cggtggacgt ctttcacgcc
      301 cagcgcggtg cgggacggcc ggccacgacc gcggcgcagg cggccagtgc cgccaagagc
      361 caggtggagc aggccgccga ccgggcgctc gcgatgctgc gccaccacga ggcgggcgtc
      421 cacgagctgt tccggaccac caacgctgtg cccggcttgg ccgtgatcgt tggcggcgag
      481 acgatccgcc gactggccgg ctcgcccgac atccgctcgg tgcacaagat cgtgcccaag
      541 accgcgatga actccagcgc cgtgcagctg accaggacgc tgcacgcctg gcagcagacc
      601 gggtggctgg gcgacggggt acggatcggc atcatcgacg acgggatcga ctacacgcac
      661 gcggacttcg gcggcccggg taccccggcg gcttacaccg ccatcgatcg caccatgatc
      721 aagccgagct acttcccgac ccgcaaggtg gtgagtgggt acgacttcgc cggcgacgac
      781 tacgacggcg gcgtggccgg tgacccgctg ggggccgtcc cacggccaga ccccaacccg
      841 ctgtcctgcg gtcaacacgg caaccacgtc gccgggaccg cggccggatt cggcgtcaac
      901 gctgacggca gcaccttccg cggtgattac cagcatctgg acccggcagc gctcaacgcg
      961 ttgcggatcg gccccggcac cgccccgcac gccttgctgt acgcgctgaa ggtcttcggt
     1021 tgccgcggtg caacagggtt gacggccaag gcgatggact gggcgctgga ccccaacggt
     1081 gatggcgact tctccgaccg gctcgacgtg gtgaacctat cgctgggctc cgattacggt
     1141 gctcccgatg acccggacag ccttttcgtc cgccagctgg cggccaacgg ggtgctcccg
     1201 gtcttcgccg caggcaacgg cggtgacctc tacgacatcg gtggagcacc gggcaacact
     1261 cccgaggccc tcaccgtggc cagtacccgg gactccgctg tgctgcgcga cggtgccgag
     1321 gtggccgcgg tctcggggga ccagatcgtg ggtggccaga tcgtcggcgg ccagtacagc
     1381 caggactttc ccagctacga cagcttggat cgcaccgcgc cggtggtaag gctgtccgat
     1441 cccgccaacg ccgacggctg ccagccctat tcggctgccg acgctgccgc ggtgatgggc
     1501 aagcacgcct ggctggagtg ggacgacgac aacgccaccc ggcgctgcgg ttcgaaggtg
     1561 cgggccgacc acgcgctggc tgccaaggcc gcaggagtgc tgctgtcctc ggcggaggag
     1621 catttcgccg cgagcatcgc aggcaatgca gggttgccga tgttccagtt gaccggtctg
     1681 gacaccgcgg cgctgcgccc ggcgctgcag gccgggacgc tcgtggtgcg gctcgcggga
     1741 aagctgcgga cctcggtgca caccgatgac ccacgaatca ccgataccgt cagtgagttc
     1801 agctcacgtg acgttcgagg tcccgctgtc aaacccgacg tggcggcgcc cggggacacc
     1861 attgcgtcgg cgctgaccgg caccggatcg gatgtcctgg cgatcagcgg gacgtcgatg
     1921 gcttccccgc acgttgcggg aattgcggcg ttggtccgcc aggcgcatcc ggactggaat
     1981 gcggccgagg tcaaggccgc ggtggtgaat accgcaggag cggatgtcca ttcgcgcgat
     2041 ggccagcagg gcccgatggc ggcccccaac cgggtcggca ccggtcgggt ggacgccgca
     2101 gccgcggtgc gcactcaggt gctggcgatg gtgcccgacg accccggcca ggtcagtgcc
     2161 ggcttcggcg tggtgcaggc caccggtcca gtcaccctca gcaagaagat cacactggtg
     2221 aacaagggtg cgaccgcggc gcggtacgcc gtcgcctacc aacccatcac caccatgccc
     2281 ggagtcgact accacgtatc cagccgctcg gtcgacatcg ccccgcgcgg ggtcgctgca
     2341 ctgaccgtca cgctacgcat cgacgatccg tcagcactgc gccggaccgc cgatccgacg
     2401 atcgagaaga cccaactcgg tgcggcccgc cagttcgtcg ccgacgcctc cggccggatc
     2461 atattcagcg cagccgactc gggtgcgggt gtcccgcaag atacggtaaa ccctgcgggt
     2521 gccggtctat gccgctgcga ggcccgtgtc cggcctgtcg gcactcgaga gtctgcactt
     2581 ccagcgtaac gaccggcagg cgtgctgccc ctgtccggcc gcggagtgga ccagggcagc
     2641 ggagatgccg cctaccggtc gctgatcagc gtcctgcagc tgcagaccga aagtggacgg
     2701 ttgggtgaat gcccggggat attcgtggcg gcgtgcacgc cgaacgggac cgctcggggc
     2761 ggtgacatcc gctacgtcgg tgtgacgtcc actgcgccgc tggcggcggc ccggggcagg
     2821 ccgaaagacg cgttgctcgc gttcgggatc acgacttggg aggactggta caacgtcggc
     2881 agcaacacca tcccgttcgt gaacatcgac accactgggg atgggttgcc cgacttcgag
     2941 gtccgcggga cgaagctgac cggtaccgac gtgctcgtcg cgtcgactgc cgatttgcac
     3001 gcgccgccag gatccccacc ggttcaggtg ctgccggtca atgggcagtt cggtgacgtg
     3061 gactcgggcg tcttcgacag caacatcatc atgctgccgg tgtcgctggc cgcgctgagg
     3121 atcgacccgt tggcggtaac ggcgccgatc agctacacca cggctgtcgc aagccattac
     3181 gccagtccgg tgagcgtcga cgaagtcatc gacagcacgg tcccggcgtt gttcgacgcc
     3241 gtgcggccgg ggctgtgggc c
//
LOCUS       DATADN010000067         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_611801, whole genome shotgun sequence.
ACCESSION   DATADN010000067 DATADN010000000
VERSION     DATADN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611801"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VHH53_00880"
     CDS             complement(<1..197)
                     /locus_tag="VHH53_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2288769.1"
                     /translation="MGCVFDALVRRVGRRFGRAEPRRRVGTYLQGLLAGLSRKNGWTV
                     AEHAGEVSPDGMQRLLRWADW"
     gene            complement(305..1201)
                     /locus_tag="VHH53_00885"
     CDS             complement(305..1201)
                     /locus_tag="VHH53_00885"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX2288770.1"
                     /translation="MIFPLVRELADDNIDLAVACRVLNVSRSGYYDWLGRPASPRQQE
                     DEYLVKLIEKVHRDSRGTYGSPRVHAELVLGLDLPVNLKRVARLMRQAGIQGLYRRRR
                     RGCTVRDPAAEPSADLVNRQFTVDEPNRLWITDITEHPTREGKLYCAAVMDAYSRLII
                     GWSIAEHMRTELVTDALGMAILRRQPQRRVDDDRTVVHSDHGSQYTSWAFGQRLRSAG
                     LLASMGTIGDCYDNAMMESFWGTLQLELLDTKEWQTRDELANAIFEWIECWYNPHRRH
                     SSIGMHSPVTFETLHTRSAQDH"
     gene            complement(1198..1488)
                     /locus_tag="VHH53_00890"
     CDS             complement(1198..1488)
                     /locus_tag="VHH53_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2288771.1"
                     /translation="MPAPHPPEFRRRAVELARERTKPIAELAKDLRISESCLRNWLAQ
                     AEVDDNGSDTQLTSKEKKELAELRRDKRRLELEVEILKRAAAYFARENVLPN"
BASE COUNT          274 a          552 c          532 g          276 t
ORIGIN      
        1 tcccagtccg cccagcgcag cagccgctgc attccgtccg gggacacctc accggcgtgc
       61 tcggcgacgg tccacccgtt tttccgactc aacccggcga gcagcccctg caggtacgtg
      121 ccaacccgcc gccgcggctc cgcccgccca aaccgccgac cgacccgccg caccagcgca
      181 tcaaagacgc agcccacccg ccaagatcca ccacgcggcg atcatcgacg tcgccgagcc
      241 tcagcctccc acgacacgcc caaagtgagg ttccccccgt agcacggaca cctccggtgt
      301 ggggtcagtg atcttgagct gaccgggtgt ggagggtctc gaatgtgacg gggctgtgca
      361 ttccgatgct ggaatggcgt cggtgcgggt tataccagca ctctatccac tcgaagatgg
      421 cgttggcaag ctcgtcacgg gtttgccatt ccttggtgtc cagcaactcg agttgcaggg
      481 tgccccagaa cgactccatc atggcgttgt catagcaatc gccgatggtg cccatcgagg
      541 cgagcagtcc ggcagaccga agtcgttgcc cgaacgccca ggacgtgtat tgcgagccgt
      601 ggtcagaatg cacgaccgtc cggtcgtcgt cgacgcgtcg ctgtggctgg cggcggagta
      661 tggccatgcc gagcgcatcg gtcacgagtt cggtgcgcat gtgctcggcg atcgaccaac
      721 caatgatgag ccgggagtag gcgtccatga ccgcggcgca gtacagcttg ccctcccggg
      781 tggggtgctc ggtgatgtcg gtgatccaca gccggttcgg ttcgtcgacg gtgaactggc
      841 ggttgaccag gtcggcgctg ggctcggcgg ccgggtcgcg gaccgtgcag ccccggcgac
      901 gccgccggta caggccctgg atgcccgcct ggcgcatcag ccgggcgacc cgcttgaggt
      961 tgaccggcag gtcgagtccc aggaccaact cggcgtgcac ccgcggcgag ccgtaggtgc
     1021 cgcgggagtc acggtgcact ttctcgatca gcttcaccag gtactcgtcc tcctgctggc
     1081 gcggggaggc ggggcggccg agccagtcgt aatagcccga ccgggacacg ttcaaaaccc
     1141 ggcaggccac cgcgagatcg atgttgtcgt cggcgagttc acggaccagc gggaatatca
     1201 gtttgggagg acgttctcac gggcgaagta ggccgctgcc cgcttgagaa tctccacctc
     1261 gagctcgagt cgccgcttgt cccgccgaag ctcggcgagc tccttcttct ccttgctcgt
     1321 cagctgggtg tcgctgccat tgtcatcgac ctccgcctgt gccagccagt tccgcaggca
     1381 cgactccgat atgcgcagat ccttggccaa ctcagcgatc ggtttggtcc gctcacgagc
     1441 caactcgacg gcacgacggc ggaactcggg cgggtgggga gccggcacgg catgatcctt
     1501 cctggtggcc agcagccacc tcaggtcggg tgtccgtgct caggggggaa gctcacggta
     1561 aatcaagcga gcgacgcacg ccaccgggcc acgaatgtct cgctgacccg catagacagg
     1621 gccagcccgg acca
//
LOCUS       DATADN010000068         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_8479, whole genome shotgun sequence.
ACCESSION   DATADN010000068 DATADN010000000
VERSION     DATADN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8479"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VHH53_00895"
     CDS             <1..809
                     /locus_tag="VHH53_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX2288772.1"
                     /translation="SLAELEHSIREFGLMQPIVVRPLPAEPAIGYPPADPAIGQLHTV
                     SYELVMGERRWRAAQQAGLDRIPAIVRCTADDAMLRDALLENIHRVQLNPLEEAAAYQ
                     QLLEDFGVTHEQLASRIGRSRPVITNTIRLLRLPVAVQRRVAVGVLSAGHARALLGLD
                     EPFAQENLATRIVAEGLSVRATEEAVTLSRSEPAQAAPKAPAPRPMQAPGLQDLAENL
                     SDAFDTRVKVELGRRKGRIVVEFGSVDDLERIVAIMTPASTSRHEDISGQ"
     gene            complement(851..1720)
                     /locus_tag="VHH53_00900"
     CDS             complement(851..1720)
                     /locus_tag="VHH53_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX2288773.1"
                     /translation="MSSALRDVGLYVEEWDIDATLVSRLTGSRPDAVVVALHGGAGEN
                     GAVQSVLEMLGVPYVGTGPGACRRAWDKPTAKDELARAGLTTPDWIALPHATFRDLGA
                     QAVLDALVARLGLPLILKPDEGGSALGVHVVHEVAELPGAMVSCLAYGDTVLAEKYVD
                     GIEVAVTVLDDPDGPRALPAVEITPKSGFYDYAARYTAGLATFHAPARLATAAAAAVA
                     DLAVAAHRLLGLRDVSRTDAVVTADGVAHFLEVNVAPGLTETSLLPMAIESDGAELGA
                     VFAGLIERAVARA"
BASE COUNT          276 a          580 c          590 g          275 t
ORIGIN      
        1 ggtccctggc tgaactcgag cattcgatcc gtgagttcgg gctgatgcag cccatcgtgg
       61 tccggccact gccagcggag ccggcgatcg ggtatccgcc ggcggacccg gctatcggcc
      121 agttgcacac cgtttcctat gagctggtca tgggtgagcg gcggtggcga gcagcgcaac
      181 aagccgggct ggaccggatc ccggcaatcg tgcgttgcac cgccgatgac gcgatgctcc
      241 gcgacgcgtt gctcgaaaac attcaccggg tgcagctcaa tccgctcgag gaggccgccg
      301 cctaccagca gctgctggag gacttcggtg tcacgcacga gcagttggca tcccggatcg
      361 ggcgcagtcg tccggtcatc accaacacca tcagactgct caggctgccg gtcgcagtgc
      421 agcggcgggt cgcggtcggc gtcctgtccg ctgggcacgc ccgcgcgctg ctcggtctgg
      481 acgagccgtt cgcccaggag aatctggcga cacgcatcgt cgccgagggc ttatcggttc
      541 gggccaccga ggaagccgtc acgctaagcc gcagtgagcc tgcccaggct gctccgaagg
      601 caccggcgcc tcgtccgatg caggcgcccg ggctgcagga ccttgctgag aacctgtccg
      661 acgccttcga cacccgggtc aaggtggaac tgggccgtcg caagggccgg atcgtggtgg
      721 agttcggatc cgtcgacgac ctggaacgca tcgtcgccat catgacccca gcgtccacca
      781 gccgacatga agacatatcg ggccaatgat ggcgggcgat acccgatatg tctccatgtc
      841 gggtatgcgt tcaggcgcgg gctacagctc gctcgatgag cccggcgaag accgcgccta
      901 gctcggcgcc gtcggactcg atcgccatcg gtagcagtga cgtctccgta agccccggag
      961 ccacgttgac ctcgaggaag tgcgcgactc cgtctgcggt caccacggca tcggtacggg
     1021 acacgtcgcg cagcccgagt aaccggtgcg cagccaccgc gagatcggcc actgcggcgg
     1081 cggctgcggt ggcgagccga gccggggcgt ggaaggtggc gagtccagcg gtgtagcgag
     1141 cggcgtagtc gtagaagccg gacttggggg tgatctccac tgccggcagc gcccgcggac
     1201 cgtccggatc gtcgagcact gtgaccgcga cctcgatgcc gtcgacgtac ttctcggcca
     1261 ggacggtgtc cccgtaggct aggcagctca ccatagcgcc cggtagttcg gcgacctcgt
     1321 gcactacgtg cacccccagc gcagagccac cttcatctgg tttgaggatc aggggtagcc
     1381 cgagccgggc gaccagcgcg tcgagcacgg cttgtgcccc gaggtcgcgg aaggtggcgt
     1441 gcggcagggc gatccagtcc ggcgtggtta acccggcgcg cgccagctcg tctttggcgg
     1501 tcggcttgtc ccaggcgcgc cggcaggctc ccgggccagt gcctacgtag ggcactccca
     1561 gcatttccag caccgactgc acggcaccgt tctcgccagc tccaccgtgt agcgccacca
     1621 cgacggcgtc tggccggctg ccagtgagcc ggctgacgag ggtggcgtcg atgtcccact
     1681 cctcgacgta cagcccgacg tctcgtaggg cactggacag c
//
LOCUS       DATADN010000069         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_913128, whole genome shotgun sequence.
ACCESSION   DATADN010000069 DATADN010000000
VERSION     DATADN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913128"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VHH53_00905"
     CDS             <1..739
                     /locus_tag="VHH53_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2288774.1"
                     /translation="TTAGVQTGVALAFLGSRVLGQYDPFGGPSEDPGRLLLVAPNIIT
                     IGTTLDVPADEFQMWVCLHEATHRLQFNAVPWLREYFAAQVQAFVAAAEDPETVAKMV
                     GRLPAALRGARRGEASDALGVLALLHGPEQRAVLERLLALATLLEGHADHVMDAVGPS
                     VVPSVRLIRQRFTARRQGGGLGERVLRALLGVDAKLRQYAEGAAFTGHVVNAVGMDGF
                     NAVWTSPETLPTRAEIIDPAAWLYRMD"
     gene            763..1731
                     /gene="tilS"
                     /locus_tag="VHH53_00910"
     CDS             763..1731
                     /gene="tilS"
                     /locus_tag="VHH53_00910"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX2288775.1"
                     /translation="MRAAVRRFLTVHRTAGVIAVACSGGADSLALAAATVHCAGRLGV
                     AVHGLVVDHGLQPESGSVAQTAAQALRALGCTAVRVLQTTVEGPGGPEAAARRARYAA
                     LRAAAPAGSLVLLGHTLDDQAETVLLGLGRGSGPRSVAGMREFVPPWGRPLLGVRRAV
                     TAAACSALGLVPWVDPHNAEPRFQRVRLRTEVLPLLEDVLAGGVASALARTARQLQED
                     LDLLDRQAEVLLAKALDIAPLDVAGFNATGLNATGLDVAVLAQAPAALRRRVLRRWLL
                     DAGVAELTDAHLRTADDLIGHWSGQGGVRLPGDVELFRAHGRLQLS"
     gene            1763..2317
                     /gene="hpt"
                     /locus_tag="VHH53_00915"
     CDS             1763..2317
                     /gene="hpt"
                     /locus_tag="VHH53_00915"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX2288776.1"
                     /translation="MYPGDIGSVLVTEEQLRCRIAELAEQIATDFRVEHCAGDLLLVG
                     VLKGAVMFMTDLARALPIPVQLEFMAVASYGSATSSSGVVRILKDLDRDISGRNVLIV
                     EDIIDSGLTLSWLLRNLASRRPASLEVCTLLRKPGAIAIEVPVRYIGFDIPNEFVVGY
                     GLDYGERYRDLPYIGTLNSKVYTD"
     gene            2606..>4488
                     /gene="ftsH"
                     /locus_tag="VHH53_00920"
     CDS             2606..>4488
                     /gene="ftsH"
                     /locus_tag="VHH53_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX2288777.1"
                     /translation="MDRKKLLRNQLLWVVAGLLLYYAFSFLLDDTRGYTKITTSQALE
                     QLASRNVTQATLEDKEQRLRLTLERPVQGSTQVLTQYPAQSTDEIATALRDSQIAQGW
                     DTKVTQQSLLFQVLIYLIPLGLLLLLLLWMMNNAQGGGNRVLNFGKSKAKQLTKDMPK
                     TTFSDVAGAEEAVEELEEIKDFLANPARYQALGAKIPKGVLLYGPPGTGKTLLARAVA
                     GEAGVPFYSISGSDFVEMFVGVGASRVRDLFEQAKQNAPCIIFVDEIDAVGRQRGAGL
                     GGGHDEREQTLNQLLVEMDGFDARGGIILIAATNRPDILDPALLRPGRFDRQIPVAAP
                     DIRGRRAILEVHSKGKPLSPDVDLHGLAKRTVGFSGADLANVINESALLTAREGGQVI
                     TGVELEEAVDRVIGGPRRKNRIISEREKKITAYHEGGHALAAWAMPDIDPVYKLTILP
                     RGRTGGHALVVPEDDKQMMTRSEMIARLVFALGGRSAEELVFHEPTTGASSDIEQATK
                     IARAMVTEYGMSARLGAVKYGREQGDPFLGRSAGQQPDYSLEVAHEIDEEVRALIEAA
                     HTEAWEILNTYRDILDELVVELLDRETLQRKDLERVFDRVEKRPRITAFNDFGGRTPS
                     DKPP"
BASE COUNT          692 a         1448 c         1583 g          765 t
ORIGIN      
        1 caccaccgca ggggtgcaga ccggggtcgc gctggccttc ctcggctctc gggtactggg
       61 gcagtacgac ccgttcggtg gaccgtccga agacccggga cggttgctgc tggtcgcccc
      121 caacatcatc accatcggca ccacgctgga tgtcccggcc gatgaattcc agatgtgggt
      181 gtgcctgcac gaggccactc atcggctgca gttcaacgct gtgccgtggt tgcgggagta
      241 tttcgccgcc caggtgcagg ctttcgtcgc ggccgcggag gacccggaga ccgtggccaa
      301 gatggtcggc aggttgcccg cggcgttgcg cggtgctcgg cgcggggagg catcggacgc
      361 actcggtgtg ctcgccctgc tccatggccc cgaacagcgc gcggtgctgg agcggctgct
      421 tgcgctggcc accttgctgg aggggcacgc cgaccatgtg atggacgccg tcggaccgtc
      481 ggtggtgcct tcggtgcggc tgatccgtca gcggttcacc gctcggcggc agggcggcgg
      541 tctcggcgag cgggtgctac gggcattgct gggcgtcgac gccaagctgc gccagtacgc
      601 cgaaggtgcg gcgttcaccg ggcatgtggt gaatgcggtc ggcatggacg ggttcaatgc
      661 agtgtggact tcgcccgaga cactgcccac tcgcgccgag atcatcgacc cggcggcgtg
      721 gctgtaccga atggactgag gccgcacccg gcgatcagcg aggtgcgtgc cgcggtccgc
      781 cggttcctca ccgtgcaccg gaccgcaggt gtgattgcgg tggcctgctc cggaggcgcc
      841 gactccctgg cgctggccgc cgccaccgtg cattgtgctg ggcggctcgg cgtggcggtg
      901 cacggtctgg tcgtcgacca cggcctgcag ccggaatccg ggtcggtggc gcagaccgcc
      961 gcccaggcgc tacgcgcgct cggttgcacc gctgtccggg tgctccagac cacggtggaa
     1021 ggtccgggtg gtccggaggc ggcagcccgc cgggcccggt acgcggcact gcgcgcggcc
     1081 gcgccggccg ggtcgctggt gttgctcggg cacaccctcg acgaccaggc cgaaactgtt
     1141 ctgctggggc tggggcgcgg ctctggaccc cgatcggtag ccggaatgcg ggagtttgtc
     1201 ccgccctggg gccgcccgct gctgggtgtg cgccgggccg tcaccgccgc ggcgtgcagt
     1261 gcgctggggc tggtcccgtg ggttgacccg cataacgccg aaccccggtt ccagcgagta
     1321 cggctgcgca ccgaggtgct gccgctgctg gaggatgtgc tggccggggg agtggcgtcg
     1381 gctctggctc gcacggcccg ccagttgcag gaagatctcg acctgcttga ccgccaggct
     1441 gaggtgttgt tggcgaaggc gttggacatc gccccgttgg acgtcgccgg gttcaatgcc
     1501 acggggttga atgccacggg gttggacgtg gcggtgctgg cgcaggcccc ggccgcgttg
     1561 cgccgccggg tgctgcggcg ttggttgctc gacgccgggg tcgccgagct gaccgacgcc
     1621 cacctgcgca cggcagacga cctcatcggc cactggtcgg gccagggcgg ggtgcggttg
     1681 ccgggggatg tcgagctgtt ccgagcgcat gggaggctgc agctttcctg aggtccgcac
     1741 acccccgagg gggaatcccg tggtgtatcc gggtgatatc ggctccgtgc tcgtcaccga
     1801 ggagcagctc cgatgccgca ttgcggagct cgccgagcag atcgccaccg actttcgggt
     1861 cgaacactgc gcgggcgacc tgctgctcgt cggtgtgctc aaaggcgcgg tgatgttcat
     1921 gaccgacctc gcccgggcgc tgccgatccc ggtgcagctg gaattcatgg cggtcgcctc
     1981 gtacggctcg gcgacgtcct cctcgggcgt cgtcaggatc ctcaaggacc ttgaccgcga
     2041 tatctccggt cgcaacgtgc tgatcgtgga ggacatcatc gactccggct tgacgctgtc
     2101 ctggctactg cgaaacctcg cgtcccgccg cccagcctcg ttggaggtgt gcacgctgct
     2161 gcgcaagccg ggtgcgatcg cgattgaagt gccagtgcgc tacatcggct tcgacatccc
     2221 caacgagttc gtcgtcggtt acggcttgga ctacggtgag cggtaccgcg atctacccta
     2281 catcggcacc ctcaactcca aggtctacac cgactagcgg ttcccaacat gccctgcggt
     2341 ggtgtacgcc gggtacggcc gtctgttgca cagcccgagg tgcgacccaa gtgcctaagg
     2401 gaacactgtg cgcccagggg aacactggcg ggggttccac cgttgaccgg gcatcggctc
     2461 cggttcgtcc cggaccggcg ggcggacccg cccggtaggg tggatcgcac gggagcggtc
     2521 tgctgtcctg cagtgtcctt ggacggtggg tccctaccat cgacgtcagc tcaggagggt
     2581 tgaggccgcc gagggccgta gccgaatgga tcgcaagaaa ttactccgca accagttgct
     2641 gtgggtcgtg gccgggttgc tgctctacta cgcattcagc ttcctcctcg acgacacccg
     2701 tgggtacacc aagatcacca catcgcaggc gttggagcag cttgcctcgc gcaacgtcac
     2761 gcaggcgacg cttgaggaca aggagcagcg gctgcgcctc acgctggagc gcccggttca
     2821 gggcagcact caggtgctca cccagtaccc ggcgcagagc accgacgaga tcgccacggc
     2881 gttacgcgat tcacagatcg cccaaggctg ggacaccaag gtcacccagc agtccctgct
     2941 gttccaggtg ctgatctatc tgatcccgct gggtctgctg ctcctgctgc tgctgtggat
     3001 gatgaacaac gcccagggtg gcggaaaccg ggtgctcaac ttcggcaagt caaaggccaa
     3061 gcagctgacc aaggacatgc ccaagacgac cttctcggac gtcgcgggcg cggaggaagc
     3121 cgtcgaggag cttgaggaga tcaaggactt cctcgccaac cctgcccgtt accaggcgct
     3181 cggcgccaag attcccaagg gcgtgctgct ctacggtccc cccggcaccg gtaagacatt
     3241 gctggcccgg gccgtagccg gcgaggccgg tgtgccgttc tattctattt ccggttcgga
     3301 cttcgtggag atgttcgtcg gtgtgggtgc cagccgcgtc cgggatttgt ttgagcaggc
     3361 caagcagaac gccccgtgca tcatcttcgt cgacgagatc gatgcggtgg gccgccagcg
     3421 cggtgccgga ctcggcggcg gacacgacga acgggagcag accctcaacc agctgctggt
     3481 cgagatggac ggtttcgacg cccgcggcgg catcatcctc atcgccgcga ccaaccgccc
     3541 ggacatcctg gacccggcgt tgctgagacc aggccgcttc gaccgacaga tcccggtcgc
     3601 tgcgccggac atccgcggcc gtcgcgcgat tctggaggtg cactccaagg gcaagccgct
     3661 gagccccgac gtcgacttgc atgggttggc caaacgcacc gtgggcttct ccggagcgga
     3721 tctggccaac gtgatcaacg agtccgcgct gctcaccgcc agggaaggcg gccaggtcat
     3781 caccggcgtc gagctggagg aggcggtcga ccgggtgatc ggcggtcctc gccgcaagaa
     3841 ccggatcatc tccgagcggg agaagaagat cactgcttac cacgagggtg ggcacgcgtt
     3901 ggcggcttgg gcgatgcccg acatcgatcc ggtgtacaag ttgacgattc tgcctcgtgg
     3961 acgcaccggc gggcacgcac tggtggtccc cgaggacgac aaacagatga tgacccgatc
     4021 cgaaatgatc gcccggctgg tgttcgcact gggtggacgt tcggcggagg aactggtgtt
     4081 ccatgagccg accaccgggg cgtcctcgga catcgagcag gccaccaaga tcgcccgtgc
     4141 gatggtcacc gagtacggga tgagtgcccg gctcggtgcg gtgaagtacg gccgcgagca
     4201 gggtgacccg ttcctcggcc gctccgcagg ccagcagccg gactactcac tggaggttgc
     4261 gcacgagatc gacgaggagg tgcgggcgct catcgaggca gcgcacaccg aggcgtggga
     4321 gatcctcaac acctatcggg acatcctcga tgagctggtc gtggaactgc tggacaggga
     4381 gacgctccag cgcaaggacc ttgagcgggt ctttgaccgg gtggagaagc gaccgcggat
     4441 caccgcgttt aacgacttcg gcgggcgcac cccttcggat aagccgcc
//
LOCUS       DATADN010000070         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_8606, whole genome shotgun sequence.
ACCESSION   DATADN010000070 DATADN010000000
VERSION     DATADN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8606"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..>1575)
                     /locus_tag="VHH53_00925"
     CDS             complement(27..>1575)
                     /locus_tag="VHH53_00925"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX2288778.1"
                     /translation="DRMLPGDWITVDGDTATVNTAIVLTPGVNFEIADIATVKLTGGP
                     EVPDASAIYTGSGRLTLKNTTLSGVDRNGQPLPFEAVGRPFLEAAGGGEINATDVTIT
                     DMGVPEHGQATAEPAVGFNPGSTGSLVRTTLQRNTIGVEVSKAQGVNLDGLTVTESES
                     DGIVLNGDQGTRMANLRAEKNGGNGMFVGGEQSDRPISGLTTLNNKLYGLVVTVQKGT
                     QVSGISTEADEAGGLRLNQVENVTVTDFSATDQPIGIFTHVGTTGVVLDNIRTSGGRR
                     GVVVEKSTTNLELKNSTIEGSRVAGVNIGGKEIQVNGVQVTDAKAGVRIERGAGHVKL
                     AGVTIEGGRDGIVSSAGATDVAITDLTANHVEDGAVRLATPQAVISGGTITGGTTGIQ
                     VTGPATITAVAINGASAGLRVRSPEMVRAENVAIDATDLGLDVSPGSQVVLANSSVHA
                     LEAVRGVFIPEGTNNISLPPLNLLAAMGVPLILLAIVLEQVHSFRQRKVGGNKRHVPP
                     AISLRVG"
BASE COUNT          197 a          542 c          560 g          276 t
ORIGIN      
        1 cgtccctgga ttcgagaacg attcccctaa ccgaccctca gagagatggc cgggggcacg
       61 tgccgcttgt tgccgcccac cttgcgttgg cggaaggagt gcacctgttc cagcacgatg
      121 gccagcagga tcagcggcac acccatggcg gcgagcaggt tcagcggcgg gaggctgatg
      181 ttgttcgtgc cctcggggat gaacacgccg cggaccgcct cgagggcgtg cacgctggag
      241 ttggcgagca cgacctggct gccgggcgag acgtccagcc ccaggtcggt ggcgtcgatc
      301 gcgacgttct ccgcgcgcac catctcgggc gagcggaccc gcaggcccgc gctggcgccg
      361 ttgatcgcga ccgcggtgat cgtggcgggc ccggtgacct ggatgcccgt ggtgccaccg
      421 gtgatcgtgc cgccgctgat gacggcctgc ggggtggcga ggcggaccgc gccgtcctcg
      481 acgtggttgg ccgtgaggtc ggtgatcgcg acgtcggtgg cacccgcgga ggacacgatc
      541 ccgtcgcggc cgccctcgat cgtcacgccc gcgagcttga cgtgcccggc gccgcgctcg
      601 atgcgcaccc cggccttggc gtcggtgacc tgcacgccgt tgacctggat ctccttgccg
      661 ccgatgttga ccccggcgac ccgggacccc tcgatcgtgg agttcttcag ctccaggttg
      721 gtggtgctct tctccaccac caccccgcgg cgaccgccgc tggtgcggat gttgtccagc
      781 acgacaccgg tggtgccgac gtgggtgaag atgccgatcg gctggtcggt ggcggagaag
      841 tcggtgacgg tgacgttttc cacctggttc aggcgcagcc cgcccgcctc gtcggcctcg
      901 gtgctgatgc cgctgacctg ggtgcccttc tgcacggtca cgaccaggcc gtagagcttg
      961 ttgttcaggg tggtcaggcc gctgatgggc cggtcggact gctcgccgcc gacgaacatc
     1021 ccgttgccgc cgttcttctc ggcgcgcagg ttggccatcc gggtgccctg gtcgccgttg
     1081 aggacgatgc cgtcgctctc ggactcggtg acggtcagcc cgtccaggtt cacgccctgg
     1141 gccttggaga cctccacgcc gatggtgttg cgctgcagcg tggtgcgcac cagcgagccg
     1201 gtgctgcccg ggttgaagcc caccgcgggc tccgcggtcg cctggccgtg ctcgggcacg
     1261 cccatgtcgg tgatggtgac gtcggtggcg ttgatctcac cgccgcccgc ggcctcgagg
     1321 aacggacgac cgaccgcctc gaacggcagc ggctgaccgt tgcggtccac gcccgagagc
     1381 gtggtgttct tcagcgtcag ccggccgctg ccggtgtaga tcgccgacgc atcgggcacc
     1441 tcgggcccgc cggtcagctt caccgtggcg atgtcggcga tctcgaagtt cacacccggc
     1501 gtcaggacga tcgccgtgtt caccgtggcc gtgtcaccgt cgacggtgat ccagtcaccc
     1561 ggcagcatgc ggtcc
//
LOCUS       DATADN010000071         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1214913, whole genome shotgun sequence.
ACCESSION   DATADN010000071 DATADN010000000
VERSION     DATADN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214913"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..>1602
                     /locus_tag="VHH53_00930"
     CDS             56..>1602
                     /locus_tag="VHH53_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX2288779.1"
                     /translation="MLAVGSAASALLGSPIDAILVGSVLTGNSMLAASQQVRAERLLT
                     RLLAVQDPPARRLCDGDFETIEATQLQPGDLIEVRPGEVVPADARIVEAADVEVDEST
                     LTGESLPVAKHAAATPGAALAERSCMLYATTTVVTGTAAALVTTVGAQTEARRASQTP
                     SAEKAAVGLQTQLRELTNRALPLSFAGGALVGGLGLLRMVPLRRAVASGVAVAVAAVP
                     EGMPLVATLAQQAAARRLTRAGVLVRSPRSVEALGRVDVVCFDKTGTLSENRLRVIKV
                     HNASDATRDQVLNCAARATPPKNGDRHEHATDAAVVQAAPQARRPKGKVTYLPFRSGR
                     PFSASLVGDELCVKGAPEVVLKACGNGDRRGKAAVHEMAESGLRVIAVARRSLTTQEA
                     RAAHTSDEALISLCDKELQFIGLLGMAVTPRTEAASLLASLHEQQVGVRLITGDHPVT
                     AAAIAAELGVPVTVDQVISGADWEALSRRGQEQAVRDRLVIARMTPEHKVQVVQTLER
                     TGQVCAIV"
BASE COUNT          253 a          504 c          588 g          257 t
ORIGIN      
        1 ggcgaaagat ttgacaactg ctctgcgaca agagctttcg gatccgttga cgccggtgct
       61 cgcggtggga tcagccgcca gtgcgctgct gggttcgccg atcgacgcga tactcgttgg
      121 ctcggtgctc acgggcaact ccatgctggc cgcgtcacaa caggtccggg ccgagcggct
      181 gctgacgcgc ctgctcgccg tgcaggaccc gccggcccga cgactctgcg acggcgactt
      241 cgagaccatc gaggcgacgc agctgcagcc cggcgatctg atcgaggtcc ggcccgggga
      301 ggtcgtcccc gccgatgccc gcatcgtcga ggcggccgac gtcgaggtcg atgagtcgac
      361 gctgaccggc gagtccctcc cggtggccaa gcacgcggcc gcgacgcccg gcgccgcgct
      421 cgccgaacgc agctgcatgt tgtatgcgac gactacggtc gtcacgggaa ccgcggccgc
      481 gctggtcacc accgttggtg cacagaccga ggcgcggcgg gcatcgcaga cgccgtcggc
      541 ggagaaagcg gcggtcggcc tgcagacgca gttgcgcgaa ctgaccaacc gcgcgctgcc
      601 gctgagtttc gcagggggtg ccctcgtcgg cgggctggga ttgttgcgca tggtgccgct
      661 gcgccgcgca gtggcgagcg gggtcgcggt cgccgtggcg gcagtgcccg agggcatgcc
      721 actggtggcg accctggccc agcaggcggc ggcgcggcgg ttgaccaggg cgggcgtgct
      781 ggttcgcagc ccccgatccg tcgaagccct gggtcgcgtc gatgtggtgt gcttcgacaa
      841 gaccggcacg ctgagcgaga accggctgcg agtcatcaag gtgcacaacg catctgatgc
      901 cactcgtgat caggtgctga attgcgccgc gcgggcgact ccacccaaga acggtgaccg
      961 gcacgaacat gccaccgatg ccgcggtggt gcaggccgca ccgcaagcac gacgcccgaa
     1021 ggggaaagtc acctatctgc cgttccgatc cggccgaccg ttttcggcat cgctggtcgg
     1081 tgacgagctg tgcgtcaagg gcgctccgga ggtcgtgctg aaagcctgcg gcaacggtga
     1141 ccggcgaggc aaggctgccg tgcatgagat ggccgagtct gggctgcggg tcatcgcggt
     1201 tgctcgtcgt tctctgacca cgcaagaggc gcgagccgct cacacgagtg acgaggcgtt
     1261 gatatcgttg tgcgacaagg agttacagtt tatcggtttg ttggggatgg ccgttacccc
     1321 tcgtacggag gctgcttcgc tgctggcgtc gttgcacgaa cagcaagtcg gggtcaggct
     1381 gatcaccggt gaccaccccg tcaccgctgc cgcgatcgcc gcggagttgg gcgtgccggt
     1441 gaccgtcgat caggtgatca gcggggctga ctgggaagcg ttgtcacgtc gaggccaaga
     1501 gcaggccgtg cgggaccggt tggtgatcgc ccggatgacg cccgaacaca aggtgcaagt
     1561 ggtgcagacg ctggagcgca ccgggcaggt gtgcgcgatc gt
//
LOCUS       DATADN010000072         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_762620, whole genome shotgun sequence.
ACCESSION   DATADN010000072 DATADN010000000
VERSION     DATADN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762620"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..150
                     /locus_tag="VHH53_00935"
     CDS             13..150
                     /locus_tag="VHH53_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288780.1"
                     /translation="MATYPVSGTTVEAIVQAADTAMYRAKALGRNQVVHISDLTQTGI
                     R"
     gene            503..>2042
                     /locus_tag="VHH53_00940"
     CDS             503..>2042
                     /locus_tag="VHH53_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2288781.1"
                     /translation="MKPVLTIAAAGVLALSLAACAGQPSGPATGGAQTFANGRTFTMV
                     LPADPGNLDPHRTALSVTLQVDRFLYDSLLNTNEKGESVAGLADKWEATTTSATFTLR
                     KGITCSDGTALTASTVAANINFIGDPANASTRIGLWVPPGATATADDAAGTVTVTSPA
                     PSAFLDRSLGSTQIVCDKGMKDRELLKQNAVGTGMFTVTEAVPGDHYTLERRKDYAWG
                     PGDWKTDQQGLPDRVVLRVISNESTAANLLTSGEVNVSAFIGPDQQRLQGQQLHQRDV
                     SAMLGQLWFNEKAGLPGADEAVRRALTQALDLEQLRAALTSGTGKPATGLVSPSLSPC
                     EQSDAPAKLPTHNVEQARSALDTAGWRPGGDGIRGKDGTKLSLTFYYVTELGPTMQAT
                     AELVQRFWQAVGVEVGTRGAAQAEINQVIVGGQGSWDAVVLPLNTGLPSDLVPFFSGP
                     TPPDGTNFAGIQNSEYATAVREASAIAGTAGCARWNAAEQALIERVDIVPFANSNRPV
                     FGKGA"
BASE COUNT          350 a          739 c          690 g          263 t
ORIGIN      
        1 gcgtccatcg gaatcgcgac ctacccggta tccggcacca cggtggaagc gatagtccag
       61 gccgccgaca cagcgatgta ccgggcgaag gcactgggac gcaaccaggt tgtgcacatc
      121 agcgacctca cccagaccgg aatccggtag ccggggacag ccatccaaca gcgggccggg
      181 ggcgccgacg atcacccgtc ctcggtgctg tcccccggcc acctccagcg cgacgtccgg
      241 cggcagagcg gtagcgcggt agccgaacgc atgcggtagg ccggtcagct cctgtgacga
      301 tgctgccgcc gggtcgtacg atcgcggaca gcaggcggct cagcacccag ccttattcaa
      361 aactctgcgc gccggttgag cacaaccccg gcgagtttgg gcggccggac gaactcgatg
      421 ccgatacgtg gttgagccgc acaaagacag ccgggatccg cgccggcagg cttcggcctc
      481 taggaccccc gttggaggcg tgatgaaacc ggttctgacc atcgccgcgg ctggcgtgct
      541 ggcgctgtcc ctcgccgcgt gtgccggcca accctccgga ccggccacgg gcggcgcgca
      601 gaccttcgcc aacggcagga ccttcaccat ggtgctgccg gccgacccgg gcaacctcga
      661 cccccaccgc accgcgctgt cggtcaccct gcaggtcgac cgcttcctct acgactcgct
      721 gctcaacacc aacgagaagg gcgagtcggt cgccggcctg gccgacaagt gggaggccac
      781 caccacgagt gccaccttca ccctgcgcaa gggcatcacg tgctcggacg gcaccgccct
      841 gacggccagc acggtggcgg cgaacatcaa cttcatcggc gaccccgcca atgcgtcgac
      901 gcgcatcggc ttgtgggtgc caccgggcgc gaccgccacc gccgacgacg cggcgggcac
      961 cgtcacggtc acctcccccg ctccgtcggc cttcctggac cgctcgctcg gatcgactca
     1021 gatcgtgtgt gacaagggca tgaaggaccg cgagctgctc aagcagaacg ccgtcggcac
     1081 cggcatgttc accgtgaccg aggccgtccc cggcgatcac tacacgctcg agcgccgcaa
     1141 ggactacgcc tggggacccg gcgactggaa gaccgaccag cagggactgc cggaccgggt
     1201 ggtgctccgg gtgatcagca acgagagcac cgcggcgaac ctgctcacct ccggcgaggt
     1261 gaacgtgtcc gcgttcatcg gcccggacca gcagcggctg cagggccagc aactgcacca
     1321 gcgggacgtg tcggcgatgc tcggccagct ctggttcaac gagaaggccg gtctgcccgg
     1381 agctgacgaa gcggttcggc gggccctgac ccaggcgctg gacctcgagc aactgcgcgc
     1441 cgcgctgacc agcgggaccg gcaagccggc gaccggtctg gtctccccca gcctgagccc
     1501 gtgcgagcag agcgacgccc cggccaaact gcccacgcac aacgtcgagc aggcccggtc
     1561 ggcgctggac accgcgggct ggcgcccagg tggtgacggc atccgcggca aggacggcac
     1621 gaaactgtcg ctcaccttct actacgtgac cgagctcggc ccgaccatgc aggccaccgc
     1681 cgagctggtg cagcggttct ggcaggcggt cggcgtggag gtcggcacca ggggcgcggc
     1741 acaggccgag atcaaccagg tcatcgtcgg cggccagggc agctgggacg ccgtcgtgct
     1801 gccgctgaac accggcctgc cgagcgacct ggtgccgttc ttctccgggc cgacaccgcc
     1861 ggacggcacg aacttcgccg gcatccagaa ctccgaatac gccacggcgg tgcgggaggc
     1921 gtcggcgatc gcgggcaccg ccggctgtgc caggtggaac gcggccgagc aggcgctgat
     1981 cgagcgcgtc gacatcgttc cgttcgccaa ctcgaaccgg ccggtgttcg gcaagggcgc
     2041 ga
//
LOCUS       DATADN010000073         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_461357, whole genome shotgun sequence.
ACCESSION   DATADN010000073 DATADN010000000
VERSION     DATADN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461357"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..573)
                     /locus_tag="VHH53_00945"
                     /pseudo
     CDS             complement(22..573)
                     /locus_tag="VHH53_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502009.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
     gene            complement(573..998)
                     /locus_tag="VHH53_00950"
     CDS             complement(573..998)
                     /locus_tag="VHH53_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX2288782.1"
                     /translation="MAAVVWLVAGLLLIAAEVLSSDFVLIMLGVGALVAAGSAALDAS
                     IWVEVAAFAGASLALITVARPVLRRRLHPAAVPTNTEALVGGKAVVVSTVDSSGGKVK
                     LRGELWSARAFDETEVLEPGQTVTVMNISGATAIVLGER"
     gene            complement(1051..>1637)
                     /locus_tag="VHH53_00955"
     CDS             complement(1051..>1637)
                     /locus_tag="VHH53_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HEX2288783.1"
                     /translation="VQPMAGPTRSASGSVRVAGVRARTARASRIWVEGRHDAELVERV
                     WGHDLRVDGVVVEALDGVDDLTKRVAEFCPAPHRRLGVLVDHLVAGSKETRLVQAVRS
                     EHVLVAGHPYVDIWQAVRPTALGIPGWPDVPRAVPWKQGVCQALGWVDRSGIADPAQG
                     WCRVLGAVSGFRDLEPTLLGAVERLIDFVTAGHQ"
BASE COUNT          319 a          581 c          482 g          255 t
ORIGIN      
        1 cgatgaccct cgttctcata actgcccagc tcgagcttgc ttttccatcg agtcctgaat
       61 cgagtgcggc gggtcgatcg ctttgagctc aacccggccc acccggatgc cccaccgccc
      121 ggtcgcttcg tcgagcaccc ctgatagctg accgttgatg aagtcccgag aagtcagggt
      181 gtcctccaag ctcattccac ccactacgtt gcgtagcgtg gtggtggcca actgctcgac
      241 tgcggcgatg tagttggaga tctcatacac cgcgtccttg ggattggtga cctggaaata
      301 cacgacggta tcgatggaga cagtcaggtt gtccttggtg atcacaggct gcggcgggaa
      361 ggacaccacc tgctcacgca ggtcaatccg agctcgaatc cggtcgatga agggcaccag
      421 gaagttcagc ccaggggtga acgtgctgcg gtagcggcca agccgctcga cgaccgcggc
      481 agtggcctgc gggatcacca ccacggcctt caccacagta acgacaatcg ccagcagcag
      541 gaccgcaaca gtgacgaaca gggcaacact catcaacgct ctcccaagac gatggcggtg
      601 gcaccggaga tgttcatcac cgtaacggtc tgaccgggct cgagaacctc ggtttcatcg
      661 aaagcgcggg ccgaccacag ctccccgcgc aacttgacct tgccaccgct ggagtcgacc
      721 gtgctgacca caaccgcctt accgccgacc aaagcttcgg tgttagtcgg caccgcggcc
      781 gggtgcagcc ggcgcctgag caccggcctg gccacggtga tcagggccag cgaggcaccg
      841 gcgaaggcag caacctccac ccagatcgac gcgtccaacg ccgcggaccc ggcggccacc
      901 agcgccccaa ccccaagcat gatcagtacg aagtcactcg atagcacctc tgcagcgatc
      961 agcagcagtc cggcgaccag ccacacgaca gcggccatgt gtccatagtg gcagatctgg
     1021 cagtgggatt ctgcggtgat cgtcactgat ttactggtgg ccggcggtaa cgaaatcgat
     1081 gagccgctcg acggcgccga gcagagtcgg ctcaagatcg cgaaagccac tcacggcacc
     1141 gagtactcga caccagccct gcgccggatc cgcgatcccg cttcgatcca cccagcccag
     1201 cgcctggcac accccttgct tccagggcac cgcgcgcggc acgtccggcc agccagggat
     1261 gcctagtgcg gttgggcgca cagcctgcca gatgtcgaca taggggtgcc ccgccaccaa
     1321 gacgtgctcc gaacgcaccg cctgcaccag gcgggtttcc ttgctgccgg ccaccaggtg
     1381 atcgaccagc acaccaagcc gccggtgtgg tgcaggacag aattctgcga cccgtttggt
     1441 caggtcgtcc acgccgtcga gcgcctccac gacgacgccg tccacccgca gatcgtggcc
     1501 ccagacccgt tccacgagtt cggcgtcgtg tctaccctcc acccagatcc ggctggcccg
     1561 tgccgtccgg gcccgcaccc cggccacccg aaccgagccc gacgccgagc gggtcggtcc
     1621 agccatcggc tgcaccc
//
LOCUS       DATADN010000074         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_612178, whole genome shotgun sequence.
ACCESSION   DATADN010000074 DATADN010000000
VERSION     DATADN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612178"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VHH53_00960"
     CDS             <1..196
                     /locus_tag="VHH53_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288784.1"
                     /translation="LPAHQLAEIANFFDIYRSLDQDSATKFDGYEGAVAAQRVLTRAG
                     VNCTGAGTGEQTGPADPTDS"
     gene            701..1408
                     /locus_tag="VHH53_00965"
     CDS             701..1408
                     /locus_tag="VHH53_00965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2288785.1"
                     /translation="MVYVALGDSISIDHYAGGAGRGGASLLVRNRDDDFPQWHGRDLT
                     TQDPAVAYHLLATDGGTTSTLLDSQLRQLETSAIAPTVVTLTIGGNDLLGAYGDIQRA
                     RQVVSVVRTRVGQALDRLRSIVRRPSDPVIVGTVYDPSDGTGDASRLGLPPWPLVVDA
                     LAELNTELREAASEHGARVADIHGYFLGHGMRAGNPAQSDSRPANRDLWFCNVIEPNA
                     WGASAVRAAFWDALHAG"
BASE COUNT          320 a          560 c          544 g          302 t
ORIGIN      
        1 gctgccggcg catcagctcg ctgagatcgc caactttttt gacatctacc gcagccttga
       61 ccaggacagc gcaactaaat ttgacgggta tgagggagca gtagcagcgc agcgggtcct
      121 cactcgggcg ggggtgaact gcacaggcgc tggcaccggt gagcagaccg gccccgccga
      181 cccgacggat tcctgacgtg agcaccgcac tagcatcggc accgctgtcc accgagttgt
      241 agaaaggtcg tcgtatagct caggctcgga tacaacaacg gcactcttat gatcgacaca
      301 accactaata ttccaatacg gtgccagtgg acgcgccggc cctgaaccaa aggcgcaccc
      361 atccccgtgg ccgattctga ctatgcctac aatacgaaca gaatgtggct aacgctgcaa
      421 ctcgcggtgt ccttcgaagc ctcgtccccg ggtgacgtgg gcacatgtag atacagttta
      481 atggacagcc tgatggacaa agatccgtca acgcccgaca gtgcgacgag cgctggcgga
      541 ttcatcaggg caagctgctg tcatgcagcc actacgccgg cgggccttct tggtcaccac
      601 tgggctagcg atggccgggg ggtgcggcgg gcctggtctt gatcaccgtg cagggcaacc
      661 cttgggcaca gccgcagcag gtaacaatac cgatggacct gtggtttatg tcgcccttgg
      721 tgactccatc tccatcgacc actacgccgg cggtgccggt cgcggcggtg cgagcctgct
      781 ggtgcgcaac cgcgacgacg acttccccca atggcacggt cgggacctca cgacacagga
      841 tcccgccgtg gcttaccacc tgctcgccac cgacggcggc accaccagca cgctgctgga
      901 ttcgcaactt cggcagcttg aaacttctgc catcgcgccc acggtcgtga cattgaccat
      961 cggcggcaac gacctactcg gggcatacgg cgacatccag cgcgctcggc aggttgtgtc
     1021 ggtggtgcgc actcgtgtcg gtcaggccct ggacagactg cgcagcattg tgcggcggcc
     1081 cagtgatcca gtaatcgtcg gtaccgtcta tgaccccagt gacggcaccg gcgatgcctc
     1141 gcggctggga ctgccaccgt ggccgctggt cgtcgacgct ctggccgagc tgaacacaga
     1201 gctgcgtgag gctgcttcag agcatggggc acgagtggcc gatatccacg gttacttcct
     1261 cggccacggg atgcgcgccg ggaacccggc ccaaagtgat tcccggccag ccaaccggga
     1321 cctgtggttc tgcaatgtca tcgagcccaa tgcctgggga gccagcgcgg tgcgcgcagc
     1381 attctgggac gccctgcacg ccggttaatg tcctcagacc gacgttggca ggagggccat
     1441 acccaccgca gcgcggccag cgtcgtgtcc aggaatcccg gtagctgggc gaggcggtat
     1501 tgctcggtca gaggctttgc cgagccaacc gtgagccaga ggtgtcaccc gcctgctgca
     1561 ggggaatgtg gaaacagtga ccggaatggg tccgtcggtg gcgcgctgcg cctccgcgcc
     1621 ggcgcggaca acgctgagtg ctgtggacga cttccgcagg tccagggccg ggctctgggt
     1681 cttctcggac ttggtcccca accacatccg tcgcgcgtcc tcccca
//
LOCUS       DATADN010000075         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_8885, whole genome shotgun sequence.
ACCESSION   DATADN010000075 DATADN010000000
VERSION     DATADN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8885"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1551
                     /locus_tag="VHH53_00970"
     CDS             <1..>1551
                     /locus_tag="VHH53_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2288786.1"
                     /translation="PGAARQARRIRDRLINQVEERRNPRTKATVDQLLERYLDQFDGA
                     ASTLTLYRGYVRNHISPFLGKIKVGALDADTLDAFYAELRRCRDHCSGRPFVQHRTAV
                     EHRCDQRCGQHRCQPLGATTIRHMHFILSGAYKRAVRWKWVRDSPLRQAEPPAAPVPN
                     PHPPSAAEAARIVNEAWKDPDWGTFIWLAMTTGARRGELCALRWSRIDLTPDRAVMWL
                     RRAISKGADGWAEGDLKTHQQRRVALDPETVEVLSEHQARCRARAEALGVELAADAFV
                     FSGAPDGSTYPTPGALTQRYNRLAERLGIDTNLHKLRHYSATELVAAGVDVRTVAGRL
                     GHSGGGTTTLRTYAAWVSEADQRAAGGIATRMPSRPAPLEASQRAHRDPQSPYEKIAA
                     KLRDSILSGDLVEGDFAPTEKQLAEENQVSIGTAHRAMELLKTWGFIRSSRGRRAIIV
                     CPPELLKSVIAELPGKHSAPGARALTDARPPDEPNQLPEAATAARLWAVTLRGPGGCR
                     YPARHVCE"
BASE COUNT          274 a          510 c          518 g          249 t
ORIGIN      
        1 ggcccggagc tgctaggcag gcccgtcgga tccgtgaccg gctgatcaac caggtagagg
       61 agcgacggaa ccctcgcact aaggccacgg ttgatcagct gctggagcgc tatcttgatc
      121 agttcgacgg tgccgccagc acgttgacgc tttatcgcgg ctacgtccgc aaccacatct
      181 cgccgtttct aggcaagatc aaagttggtg cgctggatgc ggacaccctg gacgcgtttt
      241 acgcggagct gcgccggtgc cgggaccact gctcggggcg cccgttcgtc cagcaccgca
      301 ccgccgtcga gcatcgttgt gaccagcgct gcggtcagca tcgctgccag ccgctgggtg
      361 cgacgaccat ccggcacatg cacttcatcc tctcgggggc ctacaagcgc gccgtgcgct
      421 ggaagtgggt gagggacagt ccgttgcgcc aggccgagcc tcctgcggca cccgtgccca
      481 acccgcaccc gccgagcgca gctgaggcgg cccggatcgt caatgaggca tggaaggacc
      541 ccgactgggg cacgttcatc tggctggcga tgaccacggg agcgcgccgc ggtgaactat
      601 gcgccctgcg ctggtcgcgg atcgacctca ccccggaccg ggcggtgatg tggctgcggc
      661 gcgcaattag taagggtgcg gatggctggg ctgagggaga cctcaagacg caccagcagc
      721 gccgcgttgc gctcgacccg gagactgtcg aggtgctcag cgagcaccag gcccgttgcc
      781 gggcacgtgc cgaggcgctc ggcgttgagc ttgccgcgga tgctttcgtg ttctcgggtg
      841 ctccggatgg cagcacctat ccgacaccgg gggcgctcac gcagcgctac aaccgcttgg
      901 ccgagcgcct cgggatcgat acaaacctgc acaaactccg gcactactca gcgacggaac
      961 tcgtcgcggc gggtgttgac gtgcggaccg tcgcgggtcg gttagggcac tccggtggtg
     1021 gaacgaccac gctcaggacc tatgccgcat gggtgtccga ggccgaccag cgggcggctg
     1081 gcggcattgc caccaggatg ccgagtagac cagcgccgct ggaagccagc cagcgggccc
     1141 accgcgaccc gcagagcccg tacgagaaaa tcgccgcaaa gctccgtgac agtattctct
     1201 ccggagacct cgttgagggc gacttcgccc cgactgagaa gcaactcgcc gaagagaacc
     1261 aagtatctat cgggaccgcg caccgagcca tggaactgct caagacgtgg ggattcatca
     1321 gatccagccg cggtcgtaga gcaatcatcg tatgtccccc cgaactgttg aagagtgtca
     1381 tcgctgagct tccgggcaaa cattctgctc cgggtgctag ggcgctaacc gatgctcgtc
     1441 ctcctgacga gcccaaccag ttacctgagg cggcgaccgc ggcgcggcta tgggcggtca
     1501 ctcttcgcgg gcccggcggg tgccgctacc cagcccggca cgtatgcgag g
//
LOCUS       DATADN010000076         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_913951, whole genome shotgun sequence.
ACCESSION   DATADN010000076 DATADN010000000
VERSION     DATADN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913951"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VHH53_00975"
     CDS             <1..252
                     /locus_tag="VHH53_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEX2288787.1"
                     /translation="GYGASKAALDQLVRVLAAEEPDVRALAVDPGDMRTAMQAAAFPG
                     EDISDRPWPEEVVPTLRRVVDERLPSGRYTVAELKRVPV"
     gene            321..1326
                     /locus_tag="VHH53_00980"
                     /pseudo
     CDS             321..1326
                     /locus_tag="VHH53_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
     gene            1379..1621
                     /locus_tag="VHH53_00985"
     CDS             1379..1621
                     /locus_tag="VHH53_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288788.1"
                     /translation="MSSPADVLTSRFGDGLAVGPDLTAAAESAVTQALAPLHGQRPGL
                     LCMFVCGDDPDAVESAGTRAMELAGAARRWVAAPAV"
BASE COUNT          223 a          572 c          619 g          251 t
ORIGIN      
        1 ggatacggtg ccagcaaggc cgcgctcgac caattggtcc gggtgctcgc cgccgaggag
       61 cccgacgtgc gggcgcttgc ggtggatccc ggtgatatgc gcaccgcgat gcaggcagcg
      121 gctttcccgg gtgaggacat cagcgatcgc ccgtggcctg aggaggtggt gcccacgctg
      181 cgtcgggtgg tcgatgagcg gctgcccagc gggcgctaca ccgtggctga gctcaagcgg
      241 gtgccagtgt gacaccggcc gtgccgtctt tcgagctggc cgagcacctg ttggctaccg
      301 gcccaccgga agcccgtggg gtgccacggg accgggtgcg gatgctggtg gcgaccgacg
      361 gcgggctcga acaccacagc gtggccgagt tgcccacggt gctgcgggcc ggcgacctgc
      421 tggtggtgaa cacctcagac acgctgcccg ccgcgctggc aggcgtcacg gttgacggcc
      481 agctcgtcga ggtgcacctg tccacgttgg atccgggggc ggcgctggac tacctggcag
      541 cgctggcaag caccacgtcg cggtggctgg tcgaggttcg cacgcctggt ccgctcggtg
      601 gccagccgtc gctgcaggac cggaccggca cgcggatccg gctgaccggt ggcgggatcc
      661 tgcaactgga tcgctccact gcggcaggat cgaccggctc ccggttgtgg tgcggcacgc
      721 tgcgcagccc catcgcgctg ggggagtggc tgcacgaata cggcgccccg atccgctacc
      781 ggtacgccag cgccccctgg ccactgtcgg cctaccgcac cgagtacgcc gaccccaggc
      841 agcgcggaga tgcccagcgc aggccggcca ctgaccaagc ggctgctgta ccggctgcgc
      901 gctcgtggag tgcaggttgc ggcattgctg ctacacaccg gggtgtcttc cgcggagccc
      961 ggtgagccgc cgtacgcgga gtggttcgcc gtgccagctt cgaccactga agcggtacac
     1021 gcggcccgcg ctgaaggccg gcgggtgatc gcggtgggca ccaccgtggt gcgcgccctg
     1081 gagtcggtcg gagcagccgg cggcaccggt tcgggctgga ccgacctggt cgtcacgcca
     1141 gagcgcggag tcaccatggt ggacgggcta ctcaccggct ggcacgagcc ggcggcgtcg
     1201 cacctgctga tgctggcggc gccggcagca cccgacctgc tggccgcctc gtacaccgag
     1261 gcgttacgcg ctgggtaccg ctggcacgag ttcggcgacg ttcacctcat cctgccaggc
     1321 gggtgactca cggagcacaa tggagctctt cacgcagtcg tcggaaggtg gctccctcgt
     1381 gtcctcccca gcagacgtgc tcacctcccg cttcggcgac ggcttggcgg tcggccccga
     1441 cctcaccgcc gcggcggagt ccgccgtcac gcaggccctg gctccgttac acgggcagcg
     1501 acccggcctg ctgtgcatgt tcgtctgcgg tgacgatccg gatgcggtcg agtcagccgg
     1561 cacccgcgcg atggagctgg ccggagccgc acgacgctgg gttgcagcgc cggcggtgtg
     1621 atcggtgccg gccggggagt ggaggctcag cccgcgatca gcgtg
//
LOCUS       DATADN010000077         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1064909, whole genome shotgun sequence.
ACCESSION   DATADN010000077 DATADN010000000
VERSION     DATADN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064909"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1379..1876)
                     /locus_tag="VHH53_00990"
     CDS             complement(1379..1876)
                     /locus_tag="VHH53_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2288789.1"
                     /translation="MKHFAIATVAEQSADFRRVLWTGRHAQLVIMTIPPGGEIGEETH
                     EDTDQILSFLSGTGEAVVADQIHRIAPGDLVAVPAGTRHNFRNTGVNPLVLYTVYGPP
                     EHADGVVHATKEEADAAEISGRDTDARDLDTSDKDPQRYVSAVQAQISPTESGSLGIG
                     SLKRR"
BASE COUNT          344 a          648 c          655 g          393 t
ORIGIN      
        1 agcgaaacat ctgtgcaata cccctggcaa aaaatgaccg cactcttacg tcctcgcaca
       61 gaccagcgcg cgcttgcaca gctcagcgag tgcatgggcc tgctgctcca ggccgtgcga
      121 gcggatcacc tgcggttagc ctggtgtgac gttgcgcagg tcactgccgt ccgatcacca
      181 aggttctcgt tggcgggctt cccagcggcg ttacgtcgcc aagcgcagtt ccacaaccag
      241 cacgatcaaa tgcagaaact cagcgaagct gccctgacac cccagcacgg ccgacacgaa
      301 ccacgacacg acacgtgacg gtcgtcaaca cgtctggtca gcggtgatcc gcgacgttcg
      361 gcaccaagag ggccgttgcg gtggctcgtt gaccgcaatg agcagaccgt gccacccatc
      421 ccggcggggg gcgtgccccg cgtgcccgta gtcagggtcg ccaggggtta acagcggtca
      481 ctcacgtccc ttactcgccc atatcccggg tccgcattcg cgcacgtcag ccccttgatc
      541 acttcccgat cttccaagct ggccatgcgg gttcgattcc cgtcgcccgc tccacacctt
      601 ccaccaggcg aaacggcggc ttctcctgat catgggaagg gccgccgtct ccgttcgtgc
      661 cattaacgtg ccaggccaca ggcaccacgg gggaaatgga tcgcgtctcg cgcggcgcaa
      721 tggtagtcat accgatccgc atccaccgcc gcgcctggct gtccacctgg ccgtaggagg
      781 ttagggtgag ggtaagaaag gtagagggcc tgtagcggcc gccgaatact cgccccactg
      841 tgcgtttgtt cagggtcgcc ggggaagatc cagcgcgtcc tgccgacggc gtgtggagcg
      901 cttcaccggc ttgggtggtg gatccactgg cgtcagccgg tcctacaccc gcagcgaccg
      961 tagctcggca tccagttcgg ccaccaactc tgtgatctgc tcatagggag ccggtgttcg
     1021 gtggtggtgt cggtgcgtcg cattgccaat ggccgtacgc atacgccgtg ctcttcggtg
     1081 atcgccaagg tcacatccgc gctcaagggg gagcacggct cgttgtgccc gggtcagcga
     1141 ggcctaatcg acactggtgc gttgggtagt gatcatcacg ccggattcgt ccgtcgtgga
     1201 catcgggacc ggcacctccc gcctgataga tatcctcggc cggtcatggc taaccgaacc
     1261 gctgctcaag ggctctatcc tccttgaggt caggcgggtc ttggtttcgt cgttgatctg
     1321 ctcccatccg gaagctggga tcggtgcatg ttggcgtaga gatgatccgt cattgccgtc
     1381 aacgtctttt cagactgcct ataccaagcg agccgctctc ggtgggggag atctgcgcct
     1441 gcaccgcgct gacgtaccgt tgtgggtcct tgtcgctggt gtcgagatcc cgggcatcgg
     1501 tgtcgcggcc cgatatctcg gcggcgtcgg cttcctcctt ggtggcgtgc acgacaccgt
     1561 cggcgtgctc gggcgggccg tacacggtgt agaggactag cgggttgacg ccggtgttgc
     1621 ggaagttgtg ccgggttccg gccggcaccg caaccaggtc gcccggggcg atccggtgaa
     1681 tctgatcagc cacgacggcc tcaccggtac cgctgaggaa actaagaatc tggtcggtgt
     1741 cctcatgggt ctcctcgccg atttccccgc ccggagggat ggtcatgatg accagctgag
     1801 cgtgcctgcc agtccacagg acccggcgga agtcagcgct ttgctcggca acggtggcga
     1861 tggcgaagtg cttcatggct ggcgtccctt tcaacgtgag attcaactct gtatgcgacc
     1921 gtggcggcga tccaacgaac gaccgcggcg gcgtccagtg cggcgacggt ggccatcgcg
     1981 aacgcggcta gaacgccgtg ctaggtgacg tcggtggcag ccgcgaaggc gatgaggagc
//
LOCUS       DATADN010000078         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1366055, whole genome shotgun sequence.
ACCESSION   DATADN010000078 DATADN010000000
VERSION     DATADN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366055"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VHH53_00995"
     CDS             <1..1041
                     /locus_tag="VHH53_00995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX2288790.1"
                     /translation="ARRLCPQAEFVAPQFARYAEISRGVMALFREISPLVEPLSLDEA
                     FLDVGGSLRRLGMSSAGIGEWIRGQVYDTYRVTCSVGVARSKFLAKLASGLAKPDGLL
                     VVPAGGVLEFLHPLPVSALWGVGRRTAEALHRLGCRTVADVAGIPLPALRRAVGVAAA
                     QHLHELANGRDERPVVPDAPEKSVGAEQTFSHDVADRAVLRRKLLRLAERTTAALRCR
                     SLCGRTIALKVRYPDFTTISRSRTLTGPTDSVQEVFGVAAELLDSHLPPGAAVRLLGV
                     RVEQLVKGAPAEQLTLDGDNANWPDAERAADAARLKFGSAAVRLATLLEAPERSNRGD
                     RRPLPETSEWIG"
     gene            1170..1562
                     /locus_tag="VHH53_01000"
     CDS             1170..1562
                     /locus_tag="VHH53_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEX2288791.1"
                     /translation="MPLSEHEQRLLDQIERALYAEDPKFASNVRGARMRNRYHRRRIQ
                     GIAIFVLGLLLLIAGVILPVRPVGIPVISVLGFLVMFAGAVVAIFSSRGAGDGRGAAS
                     PDRSAAGQGRGTLAQRMEQRFRRRFEDH"
BASE COUNT          238 a          536 c          607 g          282 t
ORIGIN      
        1 gcccgtcggc tgtgcccgca ggccgagttc gtggccccgc agttcgctcg ctatgcggag
       61 atctcacgcg gggtgatggc actgttccgc gaaatcagcc cactggtcga gccgttgagc
      121 ttggacgagg cgttcctcga tgtcggcggg tcgctgcgca ggctggggat gagctcggcc
      181 gggatcgggg agtggatccg tggtcaggtg tatgacacct accgggtgac ctgctcggtg
      241 ggggtggccc ggagcaagtt cctggccaag ctcgcctccg gattggccaa gccggatggg
      301 ctgctggtgg tgcccgccgg cggagtgctg gaattcctgc acccgttgcc agtgtcggcg
      361 ttgtgggggg tgggccggcg caccgctgag gcgttgcatc ggttggggtg ccgcaccgtg
      421 gccgacgtgg caggcatccc actgcccgcg ctgcgccgcg cggtgggtgt cgcggctgcc
      481 cagcacctgc acgagctcgc caacggccgc gacgagcgcc cggtggtacc ggatgcaccg
      541 gagaagtcag tcggcgcgga gcagaccttc tcccacgacg tggccgaccg ggcggtgctg
      601 cgccgcaaac tactccggtt ggccgagcgc accacggctg cgttgcggtg ccgcagcctg
      661 tgcgggcgga cgatcgcgct gaaggtccgt tacccggact tcaccaccat cagccgctct
      721 cgaactctga ccggcccgac cgattcggtg caggaggtgt tcggggtggc cgccgagcta
      781 ctggacagcc acctgccgcc tggggcggcg gtccggctgc tgggggtacg ggtggaacag
      841 ttggtcaaag gtgccccagc tgagcagctc accctcgacg gtgacaatgc gaactggccg
      901 gacgccgaac gtgccgcgga tgctgcacgg ttgaagttcg gcagcgcagc agtgcgtctg
      961 gccaccctgt tggaagcccc ggagcggtcc aatcggggtg ataggcggcc gttgcctgaa
     1021 acgagtgagt ggatcggcta gtccgcgccg ccgcggactc gtatcctcga ctgtaggagc
     1081 ccacggtggg cgcccggcga ccccaggtac taccgtcaga ctggctacca tcgtccgccg
     1141 tcgaaaatcg gagtgccgga ggaggagcga tgccactctc cgagcacgag cagcgtctgc
     1201 tcgaccagat cgagcgcgcg ctctatgcag aggaccccaa gttcgcgtcg aacgtccggg
     1261 gtgcccggat gcgcaaccgg taccaccgcc gccggatcca gggcatcgcg atcttcgtgc
     1321 tcggcttgct gctgctcatc gccggtgtga tccttccagt ccgtccggtg gggattccgg
     1381 tgatcagcgt ccttggcttc ctggtgatgt ttgccggtgc cgttgtcgcg atcttctcca
     1441 gccgtggtgc cggcgacggc cgaggcgcag catcacctga ccggtctgcc gccgggcagg
     1501 gccggggcac gctggcccaa cgaatggagc agcggttccg gcgccgtttc gaggaccatt
     1561 aacgtcctgc actcagcgtt tgcgaggcaa caccgagcgc ggaagtagcc gggtcatccg
     1621 ggtgggcggg gcgcaccggg ccaagctggt gcgcacggcg tcc
//
LOCUS       DATADN010000079         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1064986, whole genome shotgun sequence.
ACCESSION   DATADN010000079 DATADN010000000
VERSION     DATADN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064986"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(889..1266)
                     /locus_tag="VHH53_01005"
     CDS             complement(889..1266)
                     /locus_tag="VHH53_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEX2288792.1"
                     /translation="MAQKTTVTLVDDLTGEEAENISTVEFALDGVTYELDLTEENSTK
                     LHDALSEYAKAGRKIGGRRPSGRRPSRAIKGTGQAAGYNRETLKSIREWAKKNGYNVS
                     DRGRLPAGVLQAWQTAQAAMSRA"
BASE COUNT          312 a          542 c          559 g          326 t
ORIGIN      
        1 gcgcaacctt aacggcggcg gaaaccagca gctcaagacg ggtgcggtct catgcagatg
       61 ctgtacgaat cgccccaggg ttcgaacctg cgcagctagt actccagccg gtgcgcccag
      121 tgatcaggag gtgatcgtcc agcggtgatg acctcatcgg aagctcgtca tcgcctgccg
      181 gtgtgagtcc ggccgggtag ctgtcaggag gcccggtagc aggctggcgg cctggtctgg
      241 aaacgggccg ggttgaagcc cagcgtcgaa agcccctgtt gggggaagca actgagcggt
      301 ccgtagcgga agcgaagcct gccgcgtcgt tagacattga gggagagcct agccgggcgg
      361 ttcacggtga aggccatgga agcggtgaag accctggaag tgcagccgcg agggactccc
      421 ggcgtaagag ggcgcggaac gttcagaagg tggcggcggg aactgctctc cctcatcaca
      481 ccttctccca tgccggacac ggggcggttc cgacaatttc gccgaggacg ggcagtgccg
      541 agcgatctct aggtgggggt cgctgcggcc aagcacggac gactgaccga catgtccgac
      601 cgtcgctaca tcctcctgcg acgggtcggc gcagttgcac gacgcgcacc aggacaccgg
      661 ccagctccac acggaccggt agcgggcaga cctaaacagg cggctgccgg ccggtacggt
      721 ggcgagattg gccctcgacg ggctcaaact tctcggcgaa cgtgaaggga tagttgcacc
      781 ggctgagccg ctgggctgcc cagccggtgg tgcagcttct catttccaca aggtgttcgt
      841 ttgccagccg gtgggaacca ccgtttgcca tgacggcagg ccatgtgcct atgccctgga
      901 cattgctgcc tgggcggtct gccaggcctg caacaccccg gcgggcagtc ggccgcggtc
      961 gctgacgttg tagccgtttt tcttggccca ctcacggatc gacttcagcg tctcacggtt
     1021 gtagccggcg gcttgaccgg tgcctttgat ggctcggctt gggcggcgac cgctgggacg
     1081 ccgtccaccg atctttcgac ctgccttggc atactcagac agcgcatcgt gcagcttcgt
     1141 actgttttcc tcagtcagat caagctcgta ggtcacacca tcgagcgcga actcgactgt
     1201 gctgatgttc tcggcctctt caccggtcaa atcgtcgacc agcgtaactg tggttttctg
     1261 cgccatggtg aaaccacttc ctcgatctcg tttaggtgat gaaccttaac gcacccactt
     1321 gccacctcgc aaataggcac ggcaacgcgg cacgaacgga gcgggtaatg cgtttgccag
     1381 acaagctgtg ctgatgcgtc gtcgccctcg tcaagtacat ccgccgcact cggggtttgc
     1441 ccggagacgg aacctggcca gaagctcatg gatccggtcc cagcatgttg caccgcttgc
     1501 gtaagggcca gcggacaagg aaacctgatc cgccgaggtc cctgcgcagt ctctcactcg
     1561 ccaagcgcca gcctgggttt ccttaatttc atgcacggcc acagcgggga atgcggctac
     1621 gcaggaccgg gatcaatctc attctgtgct gcggggaacc cggtacgctc agccgccgtg
     1681 ggcccagtcg cagtgccgtc cgccgcatgg catccgcggt tctcgatgcc gtgatcgtc
//
LOCUS       DATADN010000080         3568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_914184, whole genome shotgun sequence.
ACCESSION   DATADN010000080 DATADN010000000
VERSION     DATADN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914184"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VHH53_01010"
     CDS             <1..335
                     /locus_tag="VHH53_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2288793.1"
                     /translation="RLVRETGKPIAQVARELGIHDGTLGNWVNADRRLRERGGGTLSE
                     DEPALPVRLRKHCAELQMRCDVLKRSVALWLLSRVRDKTHNAEHRIMPRGRRDRLLGK
                     EFMHLRSA"
     gene            complement(336..1325)
                     /locus_tag="VHH53_01015"
     CDS             complement(336..1325)
                     /locus_tag="VHH53_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2288794.1"
                     /translation="MTALAPTLQAFFTDRLVAQKNASGHTVASYRDTLRLLLVFTAER
                     TGTQPARMQVEDLQAPLIGAFLNHLEHERGNSVRSRNTRLAAIHSLFRYAALRLPDHA
                     DLIARVLAIPPKRCDHAVVSFLDKREIEALLDAPDTSRWIGRRDRALLMLTVQTGLRV
                     SELTGLRCRDVHLGHGAHVRCLGKGRKQRCTPLTSQTVAVLQAWLRERQGQPNDPLFP
                     TSHGRTLSCDAVAWLLSKHAATAQQRCPSIGTKTITPHVLRHSCAMTLLRAGVDTSVI
                     ALWLGHEKTDTVQIYVHADLALKERALARTTPLGAKPGRYRPPDALLTFLEGL"
     gene            complement(1322..2251)
                     /locus_tag="VHH53_01020"
     CDS             complement(1322..2251)
                     /locus_tag="VHH53_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2288795.1"
                     /translation="MSELRSAVDDYLTVRRALGFTLVATERLLTQFVGFAETVEADTI
                     TIDLALRWATLPPERSVTWYAQRLGTVRCFARWLQAIDLSTQVPPTDLLPTQRRRRVT
                     PYLYSDSDIADLITAAGQLRSPLIAATMQTFIGLVFATGLRRGEALGLDQSDLDPNTG
                     VLSVRQAKFHKPRQLPLHASAVVALGDYAIRRDRLCPLRRTPALFVSSTGSRLSASTV
                     SQTFQDLLRQTGIAQRAPGRRPRIHDARHTFAVNTLLGWYRDRGNVQARLPLLSTWLG
                     HTDPRHTYWYLQAAPELLALAAERLEQVMRTTS"
     gene            complement(2251..3456)
                     /locus_tag="VHH53_01025"
     CDS             complement(2251..3456)
                     /locus_tag="VHH53_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2288796.1"
                     /translation="MGNRATRVRVVGPLAPYADGFRTVLVERGYAPSSAAGQLQLMAH
                     LSRWLDQRGLTGRDLGPVAVEQFLQVRRTAGYRQWLSVRGMSPLLEYLHQAGSTPVPP
                     VVVAQTPAQLLLTAYRVYLVEERGLAASTVRNYLSVAQQFASHRDASGQAELGGVTAA
                     EVNEFVLASCRDRGVGSATILVVGLRALLRYLHLAGITPTRLAGAVPSAACWPATTLP
                     RPISQLDAGRLLHSCDRRTAVGRRDFAVLTLLLRLGLRVSEVAALELGDVDWHHGKIL
                     IRGKGRRTERLPLPADVGEAMAGWLRHGRPSGGCTRVFTTLLAPRGALRGKAVSEIVR
                     RAARRCGVEVSAHQLRHTAANDLLRAGASLPDVGQVLRHTSILNTALYAKIDHSKLAE
                     VARPWPVGA"
BASE COUNT          593 a         1164 c         1251 g          560 t
ORIGIN      
        1 tgcggctggt ccgggagacc ggcaagccga tcgcgcaagt cgcccgggaa ctgggaattc
       61 atgacgggac gctgggcaac tgggtcaacg ctgaccggcg gcttcgtgag cgtggtggcg
      121 gcacgctgag cgaggacgaa ccagctctgc ccgttcggct gcgcaagcac tgcgcggaac
      181 tgcagatgcg gtgtgatgtc ctcaagcgca gtgtggccct ttggctctta tcccgagttc
      241 gggataagac ccataatgcc gagcaccgga ttatgccgag ggggcgtcga gatcgcctgc
      301 tgggtaagga attcatgcac ctccgctcgg cataatcaga gcccctcaag gaaggtgagt
      361 agggcgtcgg gtggtcggta tcgtccgggc ttggcgccga gtggtgtggt gcgggcgagg
      421 gcacgttcct tgagggcgag gtcggcgtgc acgtagatct ggaccgtgtc tgttttctcg
      481 tggcccagcc agagcgcgat cacggaggtg tccacgcctg cgcgcaagag ggtcatggcg
      541 cacgagtgac ggagcacgtg aggcgtgatc gtcttggtgc cgatcgaggg gcagcgctgc
      601 tgggctgtgg cggcatgctt gctcaggagc cacgcgacgg catcgcagct gagcgtccgg
      661 ccgtggctgg tggggaacag cggatcgttg ggctggccct ggcgttcccg cagccatgct
      721 tgcagcactg cgacggtctg gctggtcagt ggcgtgcagc gctgcttacg tcccttgccc
      781 agacagcgga cgtgggcgcc gtgtccgagg tgaacgtcgc ggcagcgcaa cccggtcagc
      841 tcggagaccc gtagcccggt ctggacggtg agcatcagca atgcgcggtc acggcgaccg
      901 atccaccggc tggtgtccgg cgcgtcgagc agcgcctcga tctcgcgttt gtctaggaac
      961 gagacgacgg cgtggtcgca gcgtttgggt gggatggcca gcacccgggc tatcagatcg
     1021 gcatggtcgg gaaggcgcag cgcggcgtag cggaacagag agtggatcgc ggccagtcgg
     1081 gtgttgcggc tgcggacgct gttgccgcgc tcgtgttcga ggtggttcag gaacgcgccg
     1141 atcagcggcg cctgaaggtc ctcgacctgc attcgagccg gttgcgtgcc agtccgctcc
     1201 gcggtgaaga cgagcagcag tcgcaatgtg tcgcggtagg aggcgacggt gtggccactg
     1261 gcattcttct gcgccacgag gcggtcggtg aagaacgcct gcagggtcgg ggcgagcgcg
     1321 gtcatgacgt cgtcctcatt acctgttcga gccgttccgc cgcgagggcg agcagttccg
     1381 gtgccgcctg caggtaccag taggtgtgcc gtgggtcggt gtggccgagc caggtggata
     1441 gcagcggcaa tctggcctgc acgttgccgc ggtcgcgata ccagccgagc agcgtgttca
     1501 ccgcgaaggt gtgtcgggcg tcgtggatgc gcggccggcg tcccggcgcg cgttgcgcga
     1561 tacccgtctg gcgcagcagg tcctggaagg tctgcgacac ggtgctggcc gagagtcggg
     1621 aaccggtgct ggacacgaac aacgcgggcg tgcgccggag tggacacaac cgatcccgcc
     1681 ggatggcgta gtcgcccagc gccaccacgg ccgaggcgtg caacggcaac tggcggggct
     1741 tgtggaactt cgcctggcga acgctcagca caccggtgtt tgggtccaga tcgctctgat
     1801 ccagacccag ggcctcacct cgtcgcaggc cggtcgcgaa gaccagcccg atgaaggtct
     1861 gcatcgttgc cgcgatcagc ggtgagcgca gttggccggc agcggtgatc aggtcggcga
     1921 tgtcggaatc ggaatacaga tacggcgtga cccgccggcg gcgttgggtc ggcagcagat
     1981 cggtcggcgg cacctgcgtg ctcagatcga tggcctgcaa ccagcgggcg aaacagcgca
     2041 ccgtcccgag ccgctgcgcg taccaggtca ccgaacgttc cggcggcagc gtggcccagc
     2101 gcagcgccag gtcaatggtg atcgtgtcag cctcgaccgt ctcggcgaag ccgacgaact
     2161 gtgtcagtaa ccgctcggtg gccacaagcg tgaaccccag cgcgcggcgc accgtcaggt
     2221 agtcgtccac cgcgctacgt agctcgctca tcacgcaccc accggccacg gccgagcaac
     2281 ctcggccagc ttggaatggt cgatcttggc gtagagcgcg gtgttcagga tgctggtgtg
     2341 ccgcaagacc tgacccacgt cgggcaggct ggcgcccgcg cgcagcaggt cgttcgcagc
     2401 tgtgtgccga agctgatgcg ccgacacttc gacgccgcag cggcgggcgg ctcgccgcac
     2461 gatctccgag acggccttac cacgcagcgc tccacgcggg gcgagcagcg tggtgaacac
     2521 gcgcgtgcag ccgcccgatg gccggccgtg gcgcagccaa cccgccatcg cctcaccgac
     2581 atcggctggc agcggcagcc gttcggtgcg ccggccctta ccacggatca agatcttgcc
     2641 gtggtgccaa tccacatcac ccagctccaa cgcagccact tcgctgaccc gtaacccgag
     2701 ccgcagcagc agcgtgagga ccgcgaagtc gcggcggcct accgccgtcc gccggtcaca
     2761 cgagtgcagc agccgccccg cgtcgagctg gctgatcggt ctgggcagcg tcgtcgctgg
     2821 ccagcacgcc gccgacggta cggcgccagc caacctcgtc ggtgtgatcc cggccaaatg
     2881 caaatagcgc agtaacgccc gcaacccaac aacaaggatc gtggccgaac cgacgccgcg
     2941 gtcccgacag ctcgccagca cgaactcgtt cacctcggcc gcagtcacgc cgcccagctc
     3001 cgcctgacct gatgcgtcac ggtgcgaggc gaactgttgc gcgacagaca ggtagttgcg
     3061 caccgtggac gcggccaacc cgcgctcctc gaccaggtag acgcggtagg cggtaagcag
     3121 cagctgtgcc ggcgtctggg ccaccaccac gggtgggacc ggcgtggacc cggcttgatg
     3181 cagatactcc agcagcgggc tcatgccccg caccgacagc cactgccggt acccggcggt
     3241 gcgacggacc tgcaggaact gctccacggc gacaggcccc aagtcacggc cagtcaagcc
     3301 acgctgatcc agccatcggc tcaggtgcgc catcagctgc agctgcccgg cggccgagct
     3361 cggtgcgtac ccccgctcca ccagtaccgt ccggaaccca tccgcgtacg gcgccagcgg
     3421 cccgaccacc cgcacccgcg tcgctctgtt gcccatgttc cgctcctctc cagatgtgct
     3481 tgatggagag aagtctcgtc agcagacgag ccggattatg ccgagcggag gtgcatgaat
     3541 tccttaccca gcaggcgatc tcgacgcc
//
LOCUS       DATADN010000081         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_462451, whole genome shotgun sequence.
ACCESSION   DATADN010000081 DATADN010000000
VERSION     DATADN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462451"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VHH53_01030"
     CDS             complement(<1..815)
                     /locus_tag="VHH53_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX2288797.1"
                     /translation="MSQSPLLDVLAHRVLVADGAMGTMLQSVPLSLDDFAGLEGCNEI
                     LNVTRPDVVRAVHRSYLEAGADAVESNTFGANLANLAEYDIPDRIRELAEAGAVLARE
                     VADEFSDPDRPRFVLGSVGPGTKLPTLGHISFAELRDAYVEQVRGLLAGGADAVLVET
                     SQDLLQCKAAVIAARRAMVAEGRHVPIITQVTVETTGTMLLGTEIGAALTALEPLGID
                     LIGLNCATGPAEMSEHLRQLSKHSRIPLSVMPNAGLPELGTDGAVYPLTPDELA"
     gene            950..1822
                     /locus_tag="VHH53_01035"
     CDS             950..1822
                     /locus_tag="VHH53_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2288798.1"
                     /translation="MLPQLTSPVLVAAFQGWNDAGDAASTAIEHLQLSWDATPLAELD
                     PEDYYDFQVTRPTTKLVDGITRLVEWPTTRLSVCHPPGADYDVVLVHGIEPNMRWRSF
                     CEELLRYVQELEITTVVTLGALLADTPHTRPVPVTGTSYDAASAARFKLERSKYEGPT
                     GIVGVFQDACVQAGVPAISFWAAVPHYVSAPPSPKATLALLHRVEEVLDLEVPLGALP
                     EESEEWEATVSEMADEDDEVASYVRALEERGDTELTLHEASGDAIAADFERYLRRRGG
                     SGGAPGPRNPRDNR"
BASE COUNT          285 a          631 c          647 g          281 t
ORIGIN      
        1 gccagctcat cgggggtcag cgggtagacc gctccgtcgg tgccgagctc gggcagcccg
       61 gcgttgggca tcaccgacag cgggatccgg gagtgcttgg acagctggcg caggtgctcg
      121 ctcatctcag ccgggccggt ggcgcagttc agcccgatca ggtcgatgcc caggggttcc
      181 agcgcggtca gcgctgcgcc gatctcggtg cccagcagca tcgtcccggt ggtctccacg
      241 gtgacctggg tgatgatagg gacgtgccgg ccctcggcga ccatcgcccg ccgtgcggcg
      301 atgaccgcgg ccttgcactg cagcaggtcc tggctggtct ccaccaacac cgcgtcggcg
      361 ccgccggcga gcaggccgcg aacctgctcg acgtaggcgt cgcgcagctc ggcgaagctg
      421 atgtggccca gggtgggcag cttggtgcca gggcctaccg agccgagcac gaaccgcggc
      481 cggtccggat cggagaactc gtcggccacc tcgcgggcca gcacggcgcc cgcctcggcg
      541 agctcccgga tccgatcagg gatgtcgtac tcggcgaggt tggcgaggtt ggcgccgaag
      601 gtgttggact ccaccgcgtc ggcaccggct tccaggtagc tccggtgcac ggcacggacc
      661 acgtctggtc gcgtcacgtt gaggatctcg ttgcagccct ccagcccagc gaaatcgtcc
      721 aggctcagcg ggacggactg cagcatcgtg cccatggccc catccgcgac cagcacccga
      781 tgcgccagga cgtcgagcaa tggtgattgg gacatgagat ccagggtaga gcaggcgata
      841 tcgggatccc gggtagcgca tgagggccat tacggccccg gggccgtagg ctggcgcagt
      901 gacccatccc gacagttccg gcgacggtcc ggccgaccgc ggatcgcagc tgctgccgca
      961 gctcacctcc ccggtgttgg tggccgcgtt tcagggttgg aacgacgccg gggacgctgc
     1021 cagcaccgcg atcgagcact tgcagttgag ctgggacgcc acaccgctgg ctgagctcga
     1081 ccctgaggac tactacgact tccaggtcac ccggccgact accaaactgg tcgacgggat
     1141 cacccggctg gtcgagtggc ccaccacccg gttgtcagtg tgccatccgc ccggggcgga
     1201 ctacgacgtg gtcctggtgc acggtatcga gcccaacatg cgctggcggt cgttttgcga
     1261 agagctgctg cgctacgtcc aggagctgga gatcaccacg gtggtgacct tgggcgcact
     1321 gctcgctgat accccgcaca cccggccggt cccggtcacc ggcacctcct acgacgctgc
     1381 gtcagcggcc cgcttcaagc tcgaacggtc caagtacgag ggtccgaccg ggatcgtggg
     1441 agtcttccag gacgcctgcg tgcaggccgg ggtaccggcc atctcgttct gggccgcggt
     1501 gccgcactac gtctccgccc cgccgtcgcc caaggccacc ctggcgctgc tgcaccgggt
     1561 ggaagaggtg ctcgatctcg aggtcccgct cggcgcgctg ccggaggagt ccgaggagtg
     1621 ggaggcgacg gtcagcgaga tggctgacga ggatgacgag gtcgccagct acgtgcgggc
     1681 gctggaggag cgcggcgaca ccgagctgac cttgcacgag gccagcgggg acgccatcgc
     1741 cgcagacttc gagcgctacc tgcgccgccg tggaggttcc ggcggcgcac caggtccccg
     1801 aaatccccgc gacaaccgct gacccgttta cccgcattca gcgg
//
LOCUS       DATADN010000082         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_10078, whole genome shotgun sequence.
ACCESSION   DATADN010000082 DATADN010000000
VERSION     DATADN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10078"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1083
                     /gene="mraY"
                     /locus_tag="VHH53_01040"
     CDS             4..1083
                     /gene="mraY"
                     /locus_tag="VHH53_01040"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX2288799.1"
                     /translation="MRQILIAVGIALTVAILLTPVLIRLFTRQGFGHEIRADGPPSHH
                     KKRGTPSMGGVAILAGIWASYLGTHLVGVVMDGRGPSASGLLVLFLATALGAVGFIDD
                     LIKIRRARNLGLNKTAKTVGQITAAVVFGVLALQFRNADGLAPASPELSYVREIATVT
                     LPAALFVLFCVTIVYAWSNAVNFTDGLDGLAAGAMAMVCAAYVLITFWQFRNACATSP
                     GVGCYNVRDPLDLAIIAAATAGACIGFLWWNAAPAKIFMGDTGSLALGGVIAGLSVAS
                     RTEMLAVVLGALFVAEVTSVVVQILAFRTTGRRVFRMAPFHHHFELVGWAETTVIIRF
                     WLLTAIACGLGVALFYSEWLTTVGA"
     gene            1085..>1619
                     /locus_tag="VHH53_01045"
     CDS             1085..>1619
                     /locus_tag="VHH53_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2288800.1"
                     /translation="MGRGREEPQNPSDLALLVPGARVLVTGAGITGRSVSAVLEPTGV
                     RLTICDDDPLALQRLITPASVVTVAEAQAGIADYDLVVTSPGFPPTAPVLAAAAGAGV
                     PIWGDVELAWRLDQSAHYGPPRRWLVVTGTNGKTTTTSMLYAMLTAAGRRAVLCGNIG
                     NPVLDVIPEPSELLAVEL"
BASE COUNT          213 a          554 c          560 g          292 t
ORIGIN      
        1 tggatgaggc agatccttat cgcggtcggc attgcgctga ctgtcgccat cctgctcacc
       61 ccggtgctga tccggctctt cacccggcag ggcttcggcc acgaaatccg ggcggacggg
      121 ccgccgagcc accacaagaa gcggggcacg ccgtcgatgg gcggcgtggc gatcctcgcc
      181 gggatctggg ccagttatct gggcactcat ctcgtgggtg tggtgatgga cggccgcggc
      241 ccctccgcat cgggtctgct ggtgctgttc ttggccaccg cgctgggagc ggtcggattc
      301 atcgacgacc tcatcaagat ccgccgcgcg cgcaacctcg gcctgaacaa gacggccaag
      361 accgtcggtc agatcaccgc cgccgtcgtc ttcggcgtgc tcgccctgca gtttcgcaac
      421 gccgacggac tggcgccggc cagcccggag ttgtcctacg tgcgagagat cgccacggtc
      481 accctgcccg cggcgctctt cgtgctgttc tgcgtgacga tcgtctacgc gtggtccaac
      541 gcggtgaact tcaccgacgg cctggacggt ctggccgccg gtgcgatggc gatggtgtgc
      601 gccgcctacg tgctgatcac gttctggcag ttccgcaacg cgtgcgcgac cagtccgggc
      661 gttggctgct acaacgtccg cgatccgctg gacctggcga tcatcgcggc cgccacggcg
      721 ggggcgtgca tcgggtttct gtggtggaac gccgcgcccg ccaagatctt catgggcgat
      781 accggatcgc tggcgctggg cggcgtgatc gccgggttgt cggtcgccag tcgcaccgag
      841 atgctggcgg tggtcctcgg tgcgctgttc gtcgccgagg tgacctcggt cgtcgtgcag
      901 atcctggcat ttcgcaccac cgggcgacgg gtcttccgga tggcgccgtt ccaccaccac
      961 ttcgagctgg tgggctgggc ggagacgacg gtcatcatcc ggttctggct gctgaccgcg
     1021 atcgcttgcg ggctcggcgt cgcgctgttc tacagcgagt ggctgaccac cgtcggtgcc
     1081 tgacgtgggc cgcggacgag aggagcccca aaacccctca gacctcgcgc tgttggtgcc
     1141 gggcgcccgt gtcctggtga ccggcgccgg catcaccggt cgctcggtga gcgcggtgtt
     1201 ggagccgacc ggtgtccgcc tgacgatctg cgacgacgat ccgctcgcgc tgcagcgctt
     1261 gatcacgccg gcgtcggtgg tcaccgtcgc cgaggcgcag gcgggcattg ccgattacga
     1321 cctcgtggtc accagcccgg gattcccgcc gaccgcgccg gtgctggccg cggccgcggg
     1381 cgccggcgtg ccgatctggg gtgacgtcga attggcctgg cggctggatc aatccgccca
     1441 ctacgggcca ccgcgccgct ggctcgtggt caccggcacc aacggcaaga ccacgaccac
     1501 ctcgatgttg tatgcgatgc tcaccgccgc gggccgccgc gcggtcttgt gcggaaacat
     1561 cggcaacccg gtgctcgacg tcatcccgga accatccgag ttgctcgccg tcgaactgt
//
LOCUS       DATADN010000083         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_311749, whole genome shotgun sequence.
ACCESSION   DATADN010000083 DATADN010000000
VERSION     DATADN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311749"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VHH53_01050"
     CDS             <1..358
                     /locus_tag="VHH53_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288801.1"
                     /translation="LSAIDVKHAAIAIPAINQSLTQYDESMARSRTFMLTALATNHLR
                     QGDVDHGVRVGRDALTLATGLQSKRVTDRLRPLELAAGKSTNADSRQLARLIRQHRSP
                     SGRLADPADDGSVQAV"
     gene            complement(308..718)
                     /locus_tag="VHH53_01055"
     CDS             complement(308..718)
                     /locus_tag="VHH53_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2288802.1"
                     /translation="MRNSSRIRPIHNGRGPMFGLEIRSDSNCSHVALIGELDLASAPH
                     LQQVLDELCRDGFQEIVVDLARLDFLSATGLEVFVHADDQLRAAGGRLILNRPGPRAR
                     RVLAITGLDTVLTLRPVTSDGLDAAVIGRVSQAS"
     gene            complement(863..>1684)
                     /locus_tag="VHH53_01060"
     CDS             complement(863..>1684)
                     /locus_tag="VHH53_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX2288803.1"
                     /translation="ALAEQIGAMTGRAVGGMIELMSSDHGSRGQRGEPSTSDQGSAVG
                     EDQLARQLSELARQLQEKQSLQDTLNGITQAAVDNVPGAQFAGLTVVKGRREVHTAAW
                     IGELVRAADQVQYDTGQGPCLNSVYEEKTVRVTDMRTEQRWPEFAKRAVDLGVRSMLS
                     IQLYVTGDNLGALNLYSEATEAFDDESEHVGLLLAAHAAIAMAGAQQQEQLVQAITTR
                     DLIGQAKGILMERHKITAEQAFTLLMRVSQLANTKLRDVAEHLTATGELAARQHQ"
BASE COUNT          279 a          572 c          535 g          298 t
ORIGIN      
        1 gctgtcggcc atcgacgtca agcacgcggc catcgccatc cccgcgatca accaatccct
       61 cacccaatac gacgagtcga tggctcgtag ccggaccttc atgctcaccg cactggcgac
      121 caaccacctg cgccaaggcg acgtcgacca cggtgtccgg gtcggtcgag acgccctgac
      181 cctggccact ggactgcagt cgaaacgggt gaccgaccgg ctgagaccgc tggaactcgc
      241 agcaggaaag tcgaccaacg cggattcacg tcagctcgcc cgcctgattc gccaacaccg
      301 ctcgccgtca ggacgcctgg ctgacccggc cgatgacggc agcgtccagg ccgtctgagg
      361 tcactggccg gagcgtcagg acggtatcca gtccggtgat cgctaggacg cggcgggccc
      421 gcggcccagg ccggttgagg atgagtcgtc caccggcggc acggagctgg tcatcggcgt
      481 ggacgaacac ttccaggccg gtagcactga ggaaatccag cctggccagg tcgaccacaa
      541 tctcctgaaa accatcccgg cacagctcat cgagaacctg ctgtagatgc ggcgcgctgg
      601 ccaggtcaag ctctccgatc agagccacgt gactgcagtt gctatccgag cggatttcca
      661 gcccgaacat cggtccgcga ccgttgtgga tcggcctgat cctggagctg ttgcgcatta
      721 gcgcccccgt ctgtgtccgt gccgcggggg ctccaggtgg accaccccgc tggtgtgctg
      781 ccgcagcggc tgatggctca acctggtacc gacaataggc aaacagcgcc gagcgcagca
      841 acccgtggcg cgcaaagtgc acctattggt gctgcctggc cgccagctca ccggtggcgg
      901 tgagatgttc ggcgacgtcg cgcagcttgg tgttcgccaa ctggctcacc cgcatcagca
      961 gtgtgaacgc ctgctcagcg gtgatcttgt ggcgctccat caggatgcct ttggcctgcc
     1021 cgatgagatc gcgggtggtg atcgcctgca ccagctgctc ttgctgctgg gcaccggcca
     1081 tcgcgatggc ggcgtgcgcg gcgagcagga gcccgacgtg ctcggactca tcatcgaatg
     1141 cttcggtggc ctcgctgtac aggttgagag cgccgaggtt gtcgccagtg acgtagagct
     1201 ggatggacag catgctgcgg acgccgagat cgacggctcg cttggcgaac tccggccacc
     1261 gctgctcggt gcgcatgtca gtgacacgga cggtcttttc ctcgtagacg gagttcaggc
     1321 acggtccctg gccggtgtcg tactgcacct gatcggctgc gcgaaccaac tcgccgatcc
     1381 acgccgctgt gtgcacctcc cggcggccct tgaccacggt cagcccggcg aactgggctc
     1441 cgggcacgtt atccaccgcg gcctgggtga tgccgttgag ggtgtcttgt agcgattgct
     1501 tctcctgcag ttgacgggcc agctcgctga gctgccgggc tagctggtcc tcgcccacgg
     1561 cgcttccctg gtcgctcgtc gagggttctc cccgctggcc gcgggaacca tgatcggaac
     1621 tcatgagctc aatcatgccg cccaccgcgc gacccgtcat cgcacctatc tgctccgcca
     1681 gagc
//
LOCUS       DATADN010000084         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_613446, whole genome shotgun sequence.
ACCESSION   DATADN010000084 DATADN010000000
VERSION     DATADN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613446"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VHH53_01065"
     CDS             complement(<1..426)
                     /locus_tag="VHH53_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2288804.1"
                     /translation="MTGILAGMRVVEGSAFVAAPLGGMTLAQLGADVIRFDPIGGGLD
                     ATRWPVTADGKSLFWAGLNKGKRSIAVDLRSPLGKELLTDLITAPGDEAGLFLTNFPA
                     AGWLDFNKLRSRRSDLIMVNIVGNPDGSSAVDYTVNPATG"
     gene            complement(471..1472)
                     /locus_tag="VHH53_01070"
     CDS             complement(471..1472)
                     /locus_tag="VHH53_01070"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX2288805.1"
                     /translation="MADNRVQVGGPYFEDLARGQVFADAPGLTLTPGHAILHQALSGD
                     RLQLPLDHELSAAVTGRREPLIHPTLVCHVAIGQTTSPSQRVRGNLFYRGLRLLRPVH
                     LGDTLRTRTEVVALRQNRPKPGRLATGLVVLRVQVANQRDEPVLDFWRCPMLPLRDPE
                     ANTGHADSFDRFSSAADPVDDLPWGWRLDAFRPTVTEPGTVYDIEVRDTVTGAPELAR
                     ATLNLAATHTDPGASAYGRRLVYGGHTISIAGAHATRALPDLVTIVAWRSCDHTGPVF
                     EGDVLRTELHVEAVEQRDGYQLLDLRAVVHGCPAEDAGDQTAAEKPVLDWRFVALAR"
     gene            complement(1504..2922)
                     /locus_tag="VHH53_01075"
     CDS             complement(1504..2922)
                     /locus_tag="VHH53_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2288806.1"
                     /translation="MAGSMSLLDVSIVNVALPSIAQSLNASVAQVQWVVSGYALAFGL
                     VLVAGGRLGDALGRRRMFLVALTGFVLTSALCGAAPSAELLVAARLLQGFTAGMLTPQ
                     NSGLIQVLFHGPERGKAFGIFGATVGLSTAIGPVLGGLILTAASGQEGWRWIFYVNVP
                     IGLIAIVAAARLVPSRQPTGQRVVSQLDIVGAVLLGAGVLCLLLPLVQVESGGLGRLW
                     WLFGLAPVLATVFLRWERRVVRRGRPPLLDTRLLSATPGYPTGAALGLLYFVGFSGIW
                     LVFALYFQHGLGYTPLQSGLAVTPFALGSAVSAAVAGRLVNRYSRWLTVTGLVAVATG
                     LAITALVLHAVPVESAAWWVALPLLVAGIGGGAVISPNVTLTLECVPPVMGGAAAGAL
                     QTGQRIGSAIGTAALAAVFYSVLSHSGRFQSAIAAALLTATGFVCLALIVAVLELRAR
                     RRRAALAELDAATVAEADMHRA"
BASE COUNT          569 a         1184 c         1066 g          446 t
ORIGIN      
        1 gccggtagca gggttgaccg tgtagtcgac ggcggacgac ccatcagggt tgccgacgat
       61 gttgaccatg atcaggtcgg agcgccgcga cctgagcttg ttgaagtcca gccaacccgc
      121 ggccgggaaa ttcgtcagga acaaccccgc ctcatcgccg ggcgcagtga tcaggtcggt
      181 gagcagctcc ttgcccagcg gcgatcgcaa gtcgaccgcg atcgagcgtt tgcccttatt
      241 caggcccgcc cagaacaggc tcttgccgtc ggcggtgacc ggccagcggg tcgcgtccag
      301 gccaccaccg atcggatcga accggatcac gtccgcgccg agctgtgcca gcgtcatccc
      361 gcccagcggt gccgccacga aggccgagcc ctcgacgacc cgcattcccg ccaggatccc
      421 ggtcattcct cacctcgatt actcgcgacc ttccgatcaa cgcgccctga tcaacgcgcc
      481 agcgcgacga accgccagtc gagcaccggc ttttccgccg cggtctgatc cccagcgtcc
      541 tccgccgggc agccgtgcac caccgcgcgc aggtcgagca gttggtaacc gtcccgctgc
      601 tcgacggcct cgacatgcaa ctcggtgcgc aacacgtcgc cctcgaacac cggaccggtg
      661 tgatcgcagc tgcgccaggc cacgatcgtg accagatcgg gcaatgcccg agtggcgtgc
      721 gccccggcga tcgagatggt gtgcccgccg taaaccaggc gccgcccgta tgccgacgcg
      781 ccgggatcgg tgtgcgtggc tgcgaggttg agggtggctc tggccagctc aggggcaccg
      841 gtcacggtgt cgcgtacctc gatgtcatac accgtgccgg gttcggtcac cgtcggccgg
      901 aacgcatcga gccgccatcc ccacggcaaa tcatcgacgg ggtcggcggc ggaagaaaat
      961 cggtcgaagc tgtccgcgtg gcccgtgttg gcctcgggat cacgcagcgg gagcatcggg
     1021 cagcgccaga agtcgagcac gggctcgtcg cgctggttgg cgacctgtac ccgcagcacg
     1081 acgagcccgg tcgccaggcg tcccggtttc ggccggttct gtcgtagtgc aacgacctcg
     1141 gtgcgggtgc gaagcgtgtc acccagatgc acggggcgca gcagccgcag gccgcggtag
     1201 aagaggttgc cgcggacccg ttgcgacggt gacgtggtct gcccgatcgc gacatggcag
     1261 accagcgtgg ggtgaatcaa cggctcgcgg cggccggtga ccgccgcgga cagctcgtgg
     1321 tccagcggca gctggagccg gtcgccggac agcgcctggt gcaggatggc gtggcccggg
     1381 gtcagtgtca ggcccggggc gtcggcaaaa acctgccctc gtgccaggtc ctcgaaatat
     1441 ggcccgccga cctgcacccg gttgtccgcc atcgtggcaa atcgtacggc aacgacgcct
     1501 ctctcaagcc cgatgcatgt cggcctcggc cactgtggcc gcgtccagtt ctgccagcgc
     1561 agcccggcgg cggcgcgccc ggagctccag cacggcaacg atcagagcca agcacacgaa
     1621 tccggtcgcg gtgagaagcg ccgcagcgat cgcgctctga aagcggccgc tgtgagacag
     1681 cacgctgtag aacaccgctg ccagcgccgc cgtcccgatc gccgatccga tccgctggcc
     1741 ggtctgcagc gcaccggccg ccgccccacc catcacgggt ggcacacact ccagcgtcaa
     1801 cgtgacgttc ggggagatca ccgccccgcc gccgataccc gccaccagca gtgggagcgc
     1861 cacccaccac gctgccgatt caacgggtac cgcgtgcagg accagggcgg taatagccaa
     1921 cccggtagcg acggcgacca gcccggtcac cgtcaaccag cggctgtacc tgttcaccag
     1981 ccggccagcc accgcagcag acaccgctga cccgagcgcg aacggtgtca ccgcgagtcc
     2041 cgactgcagc ggcgtgtacc ccaagccgtg ctgaaagtac agcgcgaaca ccagccagat
     2101 cccgctgaag cccacgaagt acagcaggcc cagcgcggcg cccgtcgggt agccaggcgt
     2161 ggcggacaac aaccgggtgt ccagcaatgg cggccggccg cggcgcacca cccgccgctc
     2221 ccagcgcagg aagaccgtgg caagcaccgg cgccagaccg aacagccacc acagccggcc
     2281 caggccgcca ctttcgacct gtacgagcgg cagtagcagg cacagcacgc cggcaccgag
     2341 cagcaccgca ccgacgatgt cgagttggga aaccactcgc tgcccggtgg gctgccggga
     2401 cggcaccagg cgcgccgctg caacgatcgc aatcagcccg attgggacgt tgacataaaa
     2461 gatccaccgc cagccctcct gcccgctggc tgcggtgagg atgagaccac ccagtaccgg
     2521 tccgatcgca gtggacagcc ccaccgtggc tccgaagatg ccgaacgctt tcccccgctc
     2581 cgggccgtgg aacagcacct ggatcaagcc tgagttctga ggtgtgagca tgccggccgt
     2641 gaagccctgc agcagccggg ccgccaccag tagttccgcg ctgggggccg ccccgcacag
     2701 cgcgctggtg agcacgaatc cggtcagcgc gacgaggaac atccgccgcc ggcccagggc
     2761 gtcgcccaac cgcccccctg ccactagcac caacccgaac gccagtgcat atccggacac
     2821 cacccactgc acctgggcca ctgaggcgtt caagctctgc gcgatggagg gcagcgcgac
     2881 gttgacgatg ctgacgtcca gcaggctcat ggaacctgcc atctgggtga ccgcgagggc
     2941 tcgccaccgg tgtggataag cgggctgatc ggtcccggac cgagtgttca ccaccctcat
     3001 ccttgttcac cgacgctgct gttggctcgg gcctcaagca ggcctcgctg ccgagggctc
     3061 ccggtacgct tcgcgcgtga gagggcacgg gtgaccgaca gccgtaccac aggtgacacg
     3121 gtgtgccggg ggttcgcgac cggcgccagg ctcgacctcg ccggcgacga ggtgcctgcc
     3181 gcccctgctt gtcgggcttc tctccggtac tgcaccgcag ccgggcagcc gcacccccgc
     3241 catgcgactg gtcgggcaat ggtcc
//
LOCUS       DATADN010000085         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1065532, whole genome shotgun sequence.
ACCESSION   DATADN010000085 DATADN010000000
VERSION     DATADN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065532"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VHH53_01080"
     CDS             complement(<1..369)
                     /locus_tag="VHH53_01080"
                     /inference="COORDINATES: protein motif:HMM:NF014750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2288807.1"
                     /translation="MSRAHEPYTPGMGRGHAGGVGGYAGRALIVDVSGPDVTTLPLDE
                     RVLRAYLGGVGLGAWLLHRLAPPRVDPLSAQAPLAFVFSPLVGTPLTTSAKFAVVAKS
                     PLTGMLNDALASSQFAISGKL"
     gene            complement(375..1235)
                     /locus_tag="VHH53_01085"
     CDS             complement(375..1235)
                     /locus_tag="VHH53_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX2288808.1"
                     /translation="MAARAGNLAAGVVLAGGRSSRMGTAKAALEWHGSTLLRRTLGVL
                     ARAIDGPLVVVRARGQPLPAIPSQADVVDDPEEGLGPLQGIAAGLAALTGRAQVAFIC
                     STDLPFLHPIFVRRVLRALNDGTDVVLPVARGFPQPLAAAYRVTLAPLVAALVAAGDL
                     RPAFLFQRCATLRLNDAALLEDPALAAADPKLDSVVNVNSPDDYRSARCHPAPHVTVQ
                     RYGALARNGHRDPCTVQAATLAGAAAAVGLALDRHVVAALNGDQISRDGEVPLANGDT
                     VAFLSADAGG"
     gene            complement(1375..2067)
                     /locus_tag="VHH53_01090"
                     /pseudo
     CDS             complement(1375..2067)
                     /locus_tag="VHH53_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT          390 a          752 c          760 g          305 t
ORIGIN      
        1 gagcttgccg gagatggcga actggctgga cgccagcgcg tcgttgagca tccccgtcag
       61 cggtgacttg gcgacgacgg cgaacttggc gctggtggtc aacggtgtac ccaccagcgg
      121 cgagaacacg aacgctagcg gcgcctgcgc gctcaacggg tcgacccgcg gtggggcgag
      181 ccgatgcagc agccaagcgc ccagccccac gccgcccagg taggcgcgca gcacccgctc
      241 gtccaacggc agcgtcgtca catcggggcc gctcacatca acgatcagcg cccgacccgc
      301 atacccccca acccctccgg cgtgtccgcg ccccataccc ggtgtgtacg gctcgtgtgc
      361 ccggctcatc gggttcaccc gcccgcgtcc gcggacagga aagccacggt gtccccgttg
      421 gcgagcggta cctcgccgtc gcggctgatc tggtcgccgt tgagggcggc aacgacgtgg
      481 cggtccaggg caagacccac cgcagcggcc gcaccagcca acgtggcggc ttggacggtg
      541 cacggatcgc ggtgcccgtt gcgggccagc gcgccgtacc gctgcaccgt cacgtgtgga
      601 gcagggtggc accgagcact gcgatagtcg tccggggagt tgacgttgac caccgagtcc
      661 agctttggat cggccgcggc cagcgctggg tcctcgagca acgcggcatc attcaagcgc
      721 aacgtggcac accgctggaa caaaaaggcc gggcgtagat cgccagccgc caccaaggca
      781 gcgaccagcg gcgccagggt gacccggtag gcggcggcga gcggctgcgg gaagccacga
      841 gcgaccggga gcactacgtc ggtgccgtcg ttaagcgcac gcagcacccg gcggacgaag
      901 atcgggtgca ggaatggcag gtcagtggaa cagatgaagg caacctgtgc tcgaccggtc
      961 agcgcggcaa gtcccgcggc gatgccctgc aggggcccca gcccctcctc gggatcatcc
     1021 accacgtcag cctgtgatgg gatcgcaggt aatggctggc cccgggcgcg tacgaccacc
     1081 agcggcccgt cgatcgcgcg ggcgagcacc cctagggtgc ggcgcaagag ggtcgagccg
     1141 tgccactcca atgcggcctt cgcggtgccc atccgagacg agcggccccc cgcaagcacc
     1201 acgccagccg ccaggttccc cgcccgcgca gccatgctct cgatcgtaac gcgcacaccg
     1261 gggcggcctc agcgatccaa ccggccaacc agagcgaagc ggtgccgagc acgatcaagt
     1321 tcgtgccagc ggaggcgtcc actccccagg ctgcgatcgc ggtccgcaga gcgttcaccg
     1381 tgccgagcgt gcagccgagg cctcacggtt gcgttggctg gcgtagtcgg cgaggacgag
     1441 cgcgccggct gggcctgcca gaggaccgag tgcggaagga acgatgaagt cgtccgcatc
     1501 gtgttctggc aatctgtggt aaccggccat ggtctccgcg agcttggtgc ggaccagggt
     1561 gaacagcccg tacagggcag tcacgctgcc gccgatcacg atgcgctgcg gtgcaattgt
     1621 gtagacgatg tcacgcagcc cggcggccag gtaggcggct tgcagcgcgg cagcttctcg
     1681 aagctcgtcg ccgtcgagtt gctcggccgg gcgcccccag cgagcggcga tcccgacccc
     1741 gcctgccatt ccctccaggc agttggcatg gaatggacaa tcgcccggga agtcatcacc
     1801 gggctggcgt ggcacgctga tatggcccat ctcagggtgg cccagcccgt gcaggacttg
     1861 gccctcaatc acagctccga cgccgatgcc ggtaccgacg gtcatataga taaatgtgtc
     1921 cagaccacgg gcggcccccc agcggccttc gccaagcgca gccccgttga cgtcggtgtc
     1981 aaacccgacc ggcacgttca gtgcactatg gatggggccg accaggtcaa gatccgacca
     2041 gccaggcttc ggggtggtgg tgatgaaccc atccccggta tggagcgatg acggtcatga
     2101 cccgctgcca cgccggggaa ttggatgcgc aacgttggcc cccgggcgga tagagaatgg
     2161 ttccaagaaa tgacatcgcc acgatggggg gcgatcgagg tgggcgg
//
LOCUS       DATADN010000086         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1366736, whole genome shotgun sequence.
ACCESSION   DATADN010000086 DATADN010000000
VERSION     DATADN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366736"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VHH53_01095"
     CDS             <1..408
                     /locus_tag="VHH53_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2288809.1"
                     /translation="ESGAVVSAAVGQVPGDGTMPPYVARRVAAVGEIWAGLHRGGARI
                     VAGTDAGIAPYKPHDVLPYGIATLTELGMTNLEALRAATSITADACGLTGRKGRLIPG
                     ADADLVAVTGNPLVDINAVHDVAAVFRAGRQVR"
     gene            complement(563..838)
                     /locus_tag="VHH53_01100"
     CDS             complement(563..838)
                     /locus_tag="VHH53_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEX2288810.1"
                     /translation="MSIRWPCRPFRVVANPPYGMSTALLRVLLAPRSRLVAANLVLQR
                     ALVRRYAEYERGQTSWRWRMRAGLVLPRYAFCPAPRVDSAVLVIRRR"
     gene            complement(1193..1759)
                     /locus_tag="VHH53_01105"
     CDS             complement(1193..1759)
                     /locus_tag="VHH53_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2288811.1"
                     /translation="MSRRIPMLAGRQRPANLGVLLREAFVALNNVVIARLADRGHGAV
                     RTAHGVVFQYLDDTGTTVSLLAERAQITKQAMAELVRHLEIHGYVERVPHPTDLRAKL
                     VLRPTVAATSSLLPSSSRPNWKIGSGTSSALIGFKHCARTCKPFSTPSNTTPPTVDLR
                     SDRMSRAQRLSPVNQPLTARWGDVFDRD"
     gene            1933..2280
                     /locus_tag="VHH53_01110"
     CDS             1933..2280
                     /locus_tag="VHH53_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2288812.1"
                     /translation="MHRHDPGEIYYVRDGEFTFYVGEGSAVQRLTAAAGDVVPLAGGT
                     PHTIRNESNADAVAFVVHAPGAPMERFTRAATTLAADGPPSMDAVLAIAAKNGIELLG
                     PIPDVSDEQVDSH"
BASE COUNT          422 a          839 c          832 g          380 t
ORIGIN      
        1 gagtcggggg ccgtggtgag cgctgcggtc ggccaggtgc ccggcgacgg gacaatgccg
       61 ccctacgttg ctcgccgtgt cgcagccgta ggcgagatct gggctgggct gcaccgtggc
      121 ggcgcccgca tcgtcgcggg aaccgacgcc gggatagccc cgtacaagcc gcacgacgtg
      181 ctgccctacg ggatcgccac gctgaccgag ctggggatga ccaacctgga ggcgctacgt
      241 gcagccacct cgatcaccgc ggacgcctgc gggctgaccg gtcgcaaggg ccggctgatc
      301 ccgggggccg atgccgacct ggtcgcagtc accggcaacc cgcttgttga catcaacgct
      361 gtgcatgacg tggccgcggt cttccgggcc ggccgccagg tgcgctgacg gttcgcggcc
      421 agaagcgaga agagggcgtt tggacctaat tcacacccct gactgggacg cgccgcggga
      481 gcgccatacc ggcggtcccg gatcgcggcg gacggtgctc ctggctatgc aacgatcgag
      541 ggttggttgt gcccgaccag agtcagcgtc ggcggatgac gaggacggcc gagtccaccc
      601 gcggcgccgg gcagaacgcg taccgtggca gcactaaccc ggcgcgcata cgccatcgcc
      661 agctcgtctg gccacgctcg tactcggcgt aacggcgcac taacgcgcgt tgtagcacca
      721 gattcgccgc cacgagccga gagcgtggcg cgagaagcac ccgcagcagc gcggtcgaca
      781 tcccgtacgg gggattggcc accacccgga acgggcggca cggccaccgt atcgacagcg
      841 cgtcggcctc actaccgtca ccgacgcctc ggcgaagcgc tcacgcagat gctggacccg
      901 actcggatgc agctcgaccg cgatcacccg ggccccgacc tgcagtaggt gctgggtcag
      961 cgcaccgtgg ccggcgccga tgtccaccac gagctcaccc ggccgcacct gggcgtcgga
     1021 gacgacgcgc gcggcccact cgtcaacaag ccggtgccaa ccccacgagc gacgggaccg
     1081 cccgatggcg gacacgacgc accatcatca tgaagatcgt cacttcgcca cggtaggtcg
     1141 acccagccct gcggacaact gaattgcact acacgaaacg ctctaccgtg actcaatccc
     1201 tgtcgaacac atcaccccaa cgcgcggtga gcggctggtt gaccgggctt aaccgctgag
     1261 ccctcgacat tcggtcggac ctgagatcaa cagtcggtgg tgtggtattc gacggtgtgc
     1321 tgaatggctt gcaggtcctc gcacagtgct tgaaaccgat cagggccgag gaggttcctg
     1381 atccgatttt ccagttcggg cgcgaactgc tgggcaatag tgatgacgtc gcggccacgg
     1441 tcggtcgcag aacgagcttg gcccgcaggt ccgtggggtg cggcacccgt tccacgtagc
     1501 cgtggatttc caggtggcga acgagttcgg ccatggcctg cttggtgatc tgggcgcgct
     1561 cggcgagcaa gctgaccgtt gtcccggtgt cgtcgaggta ctgaaacacc acaccgtgag
     1621 cggtgcgcac ggcgccatgg ccccgatcag cgagccgcgc gatgaccacg ttgttgagtg
     1681 ccacgaatgc ctcccgtagc aagacgccca ggttggcggg acgctgtcga ccggccagca
     1741 tcggaatacg cctagacatc gacaggctcc ttgtctacta ttccggatat ggacatgcta
     1801 cctgtcgact tggtcgctgc ggagcggctg cctgggcacc gacgagatca ccgttcttgc
     1861 gaccagcgcg caaaccggcg gcgccttgtt cgctgtcgag gtccggatgc cgcccggtgg
     1921 ggcccgccgg tcatgcaccg acacgatccc ggcgagattt actacgtgcg cgacggcgag
     1981 ttcaccttct acgtcggcga gggctccgcc gtgcagcggc tgaccgccgc cgccggggat
     2041 gtggtcccac tcgccggcgg aacaccgcac acgatccgca acgagtccaa cgcggacgcg
     2101 gttgccttcg tggtgcacgc gccgggcgcc ccgatggaga ggttcacgcg cgccgccacc
     2161 acccttgccg ccgacggccc accaagcatg gacgccgtcc tggcgatcgc cgcgaagaac
     2221 ggcatcgagc tgctgggccc gatcccagac gtatcggacg agcaggtcga cagccattaa
     2281 ccgacgacag ctgggtcgct tgttgcggcg ggcggcggtc ttgaaggtgc gcagtcagta
     2341 ggactcggtg ccggccagcg acgtcacctt cggaacggga ccaggttgtg gcggcggaat
     2401 cggccgatct cgccctccac gccgcccttc tcgtgcgccg cgtccttgtc cggttggcaa
     2461 aggaaggagt cga
//
LOCUS       DATADN010000087         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_462864, whole genome shotgun sequence.
ACCESSION   DATADN010000087 DATADN010000000
VERSION     DATADN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462864"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..1499)
                     /locus_tag="VHH53_01115"
     CDS             complement(372..1499)
                     /locus_tag="VHH53_01115"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEX2288813.1"
                     /translation="MLTPRARAGAVLAALTVATAAVTVSAAAPGVARTAPRGQASPVA
                     VITGAGSINETEARYLIKGTDLGIMWTDERGQILAAFGDTFGAGWAGHGSGVGDPAAI
                     DWRSNTLARSSDRNPADGMSFNDFVTDRPGHAKELLPSLKQNGVEMTKIPTGGINISG
                     RDYMAYMSVRKFTTPGRWITNYSGIAYSDNGGESWKDAPGAQRPNTAALDDNFQMIAY
                     ARHDGLVYAFGTPNGRFGAAYLARVPEHRLLDTTAYEYWTGKDWQRGTSALATPIVAG
                     PVGELSVRYDQTLRSWQMMTMDEGRRAIVVRLAPHPTGPWSDPITVATHDEYPHLYGG
                     FLNPDSHGRDIYFTMTQYERYNVSMMHATLPEDVMNSAQAP"
     gene            complement(1753..>2358)
                     /locus_tag="VHH53_01120"
     CDS             complement(1753..>2358)
                     /locus_tag="VHH53_01120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2288814.1"
                     /translation="DIPAQRIVVTPLGVDRAWFGAAAPTEALRTTLGLPPRYLLFVGA
                     AQPRKGLDVLLEAHASQPGLAPLVLAGPAGWGQAPVTSSRVHSVGYLKETHLRCVVAG
                     AAALVLPSRDEGFGLPVLEAMATGVPVVCSDLPALRDITGRLATLVPPEEPAALATAL
                     AGAGDAGWDPVGAAARKAHAARYTWQACANATVRAYRQACD"
BASE COUNT          376 a          721 c          825 g          436 t
ORIGIN      
        1 accgtgcccg aaccctgagg gacggtcgcc tcgatggtcc cggttctgtt gaccttgaac
       61 tggaccggct cgtcgccaaa caggagccgc gctgcgttgt ccacaccgtc accgctgatg
      121 agcacggggg tgttcggcag cgcagagtcc ggagtaatgc tgtcgatctt catggggctc
      181 ctttcgagag cacatagtcg cgatgcccaa gaaacacccg ctgtggagct gacgcaactg
      241 cagcgtcacc cgtcgtcgag gcgtcatcgg atctgtcggc ctcggagctt tcgacgtaat
      301 ggacgcatac caactgctca gccatagttt gcaggccaac tcggactcga tcaccgcacc
      361 tttgcggggt atcagggcgc ctgggcgctg ttcatcacat cttctggcag ggtggcgtgc
      421 atcatcgaga cgttgtagcg ctcgtattgg gtcatggtga agtagatgtc gcggccgtgg
      481 gagtcggggt tgagaaagcc gccgtagagg tgggggtact cgtcgtgggt ggcgacggtg
      541 atcggatcgc tccagggccc ggtggggtgc ggggccaacc ggaccacgat cgcgcggcgg
      601 ccttcgtcca tagtcatcat ctgccagcta cgcagggtct ggtcatagcg gaccgagagt
      661 tctccgacgg gtccggcgac gatgggggtg gcgagtgcgc tggtgccgcg ttgccagtct
      721 ttgccggtcc agtattcgta ggcggtggtg tcgaggagtc ggtgttcggg gacgcgggct
      781 aggtaggcgg cgccgaagcg gccgttgggg gtgccgaagg catagacgag gccgtcgtgg
      841 cgggcgtagg cgatcatctg gaagttgtcg tcgagcgctg cggtgttggg ccgctgggcg
      901 ccgggagcgt ctttccagct ctcgccgccg ttgtcggagt aggcaattcc gctgtagttg
      961 gtgatccacc ggccaggggt ggtgaacttg cgcactgaca tgtaggccat gtagtcgcgt
     1021 ccgctgatgt tgatgccgcc ggtggggatc ttggtcattt cgacgccgtt ttgcttgagc
     1081 gagggcagta gttctttggc gtggccggga cggtcggtga cgaaatcgtt gaacgacatg
     1141 ccgtcggcag gattgcggtc tgagctgcga gccagcgtat tcgaccgcca atcgatcgcg
     1201 gccgggtcac cgacgccact gccatgaccg gcccagccgg ctccgaaggt gtcgccgaag
     1261 gcggcgagga tctggcctcg ctcatcggtc cacatgatgc caagatcggt gcccttgatc
     1321 aggtaacgag cctcagtttc gttgatcgat cctgcgccgg tgatcacggc taccgggctg
     1381 gcttgtcccc ggggcgcggt gcgcgccacc ccgggcgccg ccgcgctgac cgtgacagcc
     1441 gcggtcgcta cggtcagggc cgctagcacc gccccggctc gagctcgtgg agtgagcatg
     1501 cctgtccccc ttctgtccgg aatgcgtcgg tctgtgggca aggcagacgt cagcgttccg
     1561 tagctacaag cgacttcccc cagcactcga agggggcaag agtgcccttt ccagcgcttc
     1621 cagtagcgag tgtgcgactc aacgtataat agcgtcgatc acgctactcc tcgtgggtga
     1681 atctgaaaaa aagatcattg actgtacgtg tccggctctg atgcttggta tgtcagccag
     1741 tggtgttcac tgtcagtcgc aggcttgtcg gtacgcgcgg accgttgcgt tcgcacaggc
     1801 ctgccaggta tagcgggcgg cgtgtgcctt ccgggcggct gcgcctaccg gatcccagcc
     1861 ggcatcgcct gcgccggcca gcgcggtggc cagcgcagca ggctcctcag gcgggacaag
     1921 cgtggcgagc ctcccggtga tgtcccgcag cgccggtagg tccgagcaca ccaccggcac
     1981 cccagtcgcc atcgcctcca gtaccggaag gccgaaaccc tcgtcgcgcg acggcagcac
     2041 caacgccgcc gcgccggcga ccacgcagcg caggtgggtt tccttgaggt aacccacgga
     2101 atgcacccga gacgaggtca ccggcgcttg accccagccg gccggcccgg cgaggacgag
     2161 cggcgcaaga ccgggttgcg acgcgtgcgc ctcgagcagt acgtcgaggc cctttcgcgg
     2221 ctgggcggcg ccgacgaaca gcaagtaccg cggcggtaac cccagcgtgg tgcgcaacgc
     2281 ttcggtgggc gcggcagcgc cgaaccaggc tcggtcgacg cccagcgggg taaccactat
     2341 tcgctgcgcg gggatgtc
//
LOCUS       DATADN010000088         5084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_764185, whole genome shotgun sequence.
ACCESSION   DATADN010000088 DATADN010000000
VERSION     DATADN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764185"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VHH53_01125"
     CDS             <1..240
                     /locus_tag="VHH53_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX2288815.1"
                     /translation="VFAWAATRTGITRLPDGVRWRHLVGVAALAGIGFTVSLFITGLA
                     FERPELQDAAKVGVLAASLLAGLLGALLLFRSQRD"
     gene            complement(285..458)
                     /locus_tag="VHH53_01130"
     CDS             complement(285..458)
                     /locus_tag="VHH53_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288816.1"
                     /translation="MRGGQPPTPANQRAVEAWEALGGGVGVALGAGLGNVLFWIVIGA
                     IAGFAIGSPDDTS"
     gene            complement(1247..2221)
                     /locus_tag="VHH53_01135"
     CDS             complement(1247..2221)
                     /locus_tag="VHH53_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2288817.1"
                     /translation="MRAAVRTSYGPPDVVRIAQVAKPTPKENQVLVKVHATTVNRTDC
                     GFRAGRPFFVRALTGLPRPKVTVLGSEFAGEVEAVGHSVTSFAVGDRVFGFKGLPFGA
                     HADYMAIPQDGFLATMPAQLSFEEAAPSTEGAHYALSFIRKAKVRSGQDVLVNGATGA
                     IGSAAVQLLKHLGANVTATCGPEHLELVKGLGADRVIDYSAQDFTRDTQRYDVVLDAV
                     GKSSFLRCRRLLKPRGVYLSSEFGPLAQNLVSALVTPLLGGRKVMMNLGRDDPRMASY
                     FREVLESGAFRPLVDRRYRLDDIVEAYRYVETGRKIGNVVISIQPSAS"
     gene            complement(3094..4062)
                     /locus_tag="VHH53_01140"
     CDS             complement(3094..4062)
                     /locus_tag="VHH53_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288818.1"
                     /translation="MPEGAKRGLLAARVRRVVVPLLLVALMATLAAPAAAARPAEVIV
                     LPGATSAEGIAAGRGATFYAGDLFAGDIFRGNLRRGTAELFIDAPAGRMAVGMAADLA
                     HGLLFVAGGFTGQGYVYDLGTGATVATYQFAVPSPTAAPPTTVINDVAVTRRGAWFTD
                     SRQAQLYFVPVSRAGMPGPTFSTLALSGPAADTSGAFNLNGIQATANGKTLIVAHSAN
                     GQLYTVDPSTGGSAIIAGVSVPNVDGIVLEGRRLWAVQNTNQVTRIRLGRHLQSGVVE
                     EVITSDLFQTPATGARFGSRLAVVNAKFDTGIPPTADQYEVVLVDA"
     gene            complement(4377..4823)
                     /locus_tag="VHH53_01145"
     CDS             complement(4377..4823)
                     /locus_tag="VHH53_01145"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX2288819.1"
                     /translation="MAIRARHRRRVRALTAALLGAVVGGILLAACGDVTPPPASPPGT
                     TRPAQSLASTTTAGARGVELGVFEVSCHTGKGITASGRPSSRETVSVDPQVVPLGTRL
                     VIDEVGTRVAADTGGAIRGRRLDIWEPSVAACTRFGRRQLRVWQAP"
BASE COUNT          818 a         1786 c         1697 g          783 t
ORIGIN      
        1 gtgttcgcct gggcggcgac gagaaccggg atcacccgcc tccccgacgg agtgcgctgg
       61 cgtcacctgg tcggagtagc tgccctagct gggatcgggt tcacggtctc gctgttcatc
      121 accggcctgg ccttcgagcg cccggagctc caggatgcgg ccaaggtcgg cgtcctggcc
      181 gcgtcgctgc tggccggcct actcggggcg ctcttgctct tccgctctca gcgggactag
      241 cgaatcacgc ggacttgcag gcaatggtcc cccggcccca gggctcagga ggtgtcgtcg
      301 ggcgagccga tcgcgaagcc ggcaatggcg ccgatgacaa tccagaacag gacgttgccc
      361 agcccggccc caagggccac gccgacaccg ccgccaagcg cctcccatgc ttcgaccgcc
      421 cgctggttcg ctggtgtcgg aggttgaccg ccgcgcatgt ccgtccttcg gctcacggcg
      481 tcgtgtcgag gggggcctcg atcggatgga cgatgaggaa tcgctccaaa acgcccctcc
      541 taactcgaac cgtcacgacc gtgcccagct cgaccatggc cctcggggtc gtccaaggcg
      601 gtacgcagct gtggaaggac ctcgacacgc aggacgtggg cgcgttcgct tagcagccgt
      661 ctgccccgct ggtcagcatc acctgtgcgg ccccatccag atccagcagc gccaagcggt
      721 caccgctcct cacggccacc tcccggcctg gacgcggagg ccaagcaaat gcgaccgccc
      781 gggcgacgga actgcgctgc ccgccggccg ttcaggttta caagcgggaa cgctgcaact
      841 ccctggtaga cctccatggc cgaagcacaa ctacggccag acgagccccg gttggcgcct
      901 agagacgtcg ctgagccact ctccagctct gacacgaccg cgaagctgcg gtaccgggcg
      961 gcgatcaagc gcacacgagc gctgtgggtc cgatcgatcc agcgcaggat ccaggccgcc
     1021 gccaccccag gtatccttcg accgtgcgga gggcgtcgac cggtctccgg cggccatgct
     1081 cgagcggccg cacccgtggt cgggccggta cggcgccagg tcgagagcgc aaggggcacc
     1141 accagcgggc cgagggtggt gccgagcagc aaccaggtga agccgtcatg gccgcgacgg
     1201 cccatggccc tccccgtcac cacgacatct ccgtcaacgc ggcgcgtcac gaggccgaag
     1261 gctggatgct gatcaccacg ttgccgatct tgcggcccgt ctcgacgtac cggtaggcct
     1321 cgacgatgtc gtccagccga tatcgccggt cgaccagcgg ccggaacgcc cccgactcca
     1381 gcacctccct gaagtagctc gccatccgcg ggtcgtccct cccaaggttc atcatgacct
     1441 tcctgccgcc aagcagcggc gtgaccaacg ccgagacgag gttctgggcc agggggccga
     1501 actccgagga caggtagacc ccgcgcggct tcaacagccg ccggcaccgc aggaacgagc
     1561 tcttgcccac cgcatccagg accacgtcgt accgctgggt gtcccgggtg aagtcttggg
     1621 cgctgtagtc gatcacccgg tcggcgccca agcccttgac cagctctagg tgctcggggc
     1681 cacaggtcgc ggtcacgttg gcgccaaggt gcttcagcag ctggaccgcg gccgatccga
     1741 tggcgccggt ggccccgttg accaggacgt cctggccgct gcggaccttg gccttcctga
     1801 tgaacgagag ggcatagtgc gccccctcgg tgctgggagc ggcctcctcg aaactcagct
     1861 gcgccggcat tgtcgccagg aacccatctt gggggatggc catgtagtcg gcatgggcgc
     1921 cgaacggcag ccctttgaag ccgaacaccc ggtcaccgac cgcaaaggag gtcacgctgt
     1981 ggccgaccgc ctcgacctcc ccggcgaact cagaccccag gaccgtcacc ttcggcctcg
     2041 gtagcccggt gagggcgcgc acgaagaagg gcctgcccgc ccggaagccg cagtcggtcc
     2101 ggttcaccgt ggtcgcgtgg accttgacca agacctggtt ctccttgggc gtcggcttgg
     2161 ccacctgggc gatccggacc acgtccggtg gaccgtagct ggtgcgaacc gctgccctca
     2221 tgaagaaact gtaaccacct cacccagctc gactccaacg cccgcttccc gagacgcaca
     2281 gatccattgc gctctgcgcg ggacggccgg cgttgagtgg accgagaacc tcttggcgcc
     2341 aacatggcca agatggcccg acgccaggcc gacggcagct ggcgctatgt gatcgacaac
     2401 gcctggggcg accaggcagt cgccgactag ccactccttg tgacccgccg gcctgcccga
     2461 cgacatgctc atctgacggc gacagctacc agctcggcca caggataagg ccgccctggg
     2521 tgaccacggc atcatggtcg agatcacctg gtagtcgact tggcgacggc cgaggccgcc
     2581 ctcgaccgcc aaccctggtg acgctacggg actcgagccc tgagctcttg catgccatgc
     2641 catccgcact gcttccgccg ctcgctgcct cgggggtgga gctgagggga cttgaaccct
     2701 gacccccggc cagctcaccg gcctcctcga agtgaccgtg gttgtccgct ggataccgct
     2761 cgtgcccgct ccttatggca cgcgggtggc acggccgacg agaacgacga cgttctcacc
     2821 tggcgggacg gctccagctc gaccgaaggg tgaggcccgt cctcctcgac taaccgcttc
     2881 gttggcaaga gcccggaggg ctcgcagcag ccgggtaggg agactcgaac tccggtccac
     2941 ctccttgaag ggtgcctgct cgcggccaca tcgggcggct gacctctgaa ttcctcagaa
     3001 ccggtcgttg ccgctggtat cccggtacgc tccccgtcac cgacctagcg tgtacccagg
     3061 tgtgtacctg gctgccggtt ggactggtcc cgatcatgcg tcgacgagaa cgacctcgta
     3121 ctggtcggcg gtgggcggga tgccggtgtc gaacttggca ttcaccaccg ccaggcgact
     3181 gccaaagcgg gccccggtgg ccggggtctg gaacaggtca ctggtaatga cctcctccac
     3241 caccccggac tgcaggtgcc ggcccagccg gattctggtc acctggttgg tgttttgcac
     3301 cgcccacagc cgccgaccct ccagcacgat gccgtcgacg ttgggcacgc tgaccccggc
     3361 gatgatcgcg ctgcccccgg tcgacggatc cacggtgtag agctgcccgt tggccgagtg
     3421 ggccacgatc agggtcttgc cgttggccgt ggcctggatg ccgttgaggt tgaaggcccc
     3481 gctggtgtcg gccgccgggc cgctgagggc cagggtgctg aacgttggcc ccggcattcc
     3541 agcccggctg accggtacga agtagagctg ggcttgccgg gagtcggtga accaggcgcc
     3601 gcggcgggtg acggccacgt cgttgatgac cgtggtgggt ggagcggccg ttggggatgg
     3661 aacggcgaac tggtaggtgg ccacggtggc accggtaccg aggtcgtaca catagccctg
     3721 gccggtgaag ccaccggcca caaacagcag cccgtgggcg aggtcggcgg ccatgcccac
     3781 ggccatgcgg ccggcggggg cgtcgatgaa caactcggcg gtgccgcggc ggaggttgcc
     3841 gcggaagatg tcgccggcga acaggtcacc ggcatagaag gtggccccgc gaccggcggc
     3901 gatgccctcg gccgaggtgg cccccggcag gacgatgacc tcggccggtc gagccgcggc
     3961 cgccggcgcg gccaatgttg ccatgagggc caccagcagc agcggcacga ccacccgccg
     4021 gacccgcgct gccagcaaac cacgcttcgc accctcgggc atccaacatc ctcctgttca
     4081 ggtgctcacc gaccgaggcg tgtcccacga cgctgtagtt ccccaacccg gcggggcgac
     4141 cgaggccccg tgcctccgtt gtgcgctatg ggccgcgccc agcagcaccg gagcgaggac
     4201 acggcaagaa gccggcgaga tggttgcaac cccccttgcc gctgggaacc agaagctgcc
     4261 ccggcaccag tagtctctag tggctgagga ggatatgccc tccggagttc tgcatagctg
     4321 cagcggtgaa agtccttgcg gccacggacc agcgacggac cggcgagggg cgtcggctag
     4381 ggtgcttgcc agacgcgcag ctgcctgcgg ccgaaccgcg tgcaggcggc caccgatggc
     4441 tcccagatat ccagccgccg acccctgatg gccccgccgg tgtccgcggc cacccgagtg
     4501 ccaacctcat cgatcaccag ccgcgtcccc aacggcacca cctggggatc gaccgagacc
     4561 gtctcccggg agctcggcct gcccgaggcc gtgatgccct tgcccgtatg gcagctcacc
     4621 tcgaagacgc caagctccac cccgcgagcg ccggccgtag tcgtcgacgc caacgactgc
     4681 gcaggccggg tcgtgcccgg cggggaagca gggggtgggg tcacgtcacc gcaggccgcc
     4741 aagaggatcc cacccacgac agcaccgagc agcgccgcgg tcagtgcccg cacgcggcgt
     4801 cggtgtcgag cgcggatcgc catgtcgtcg tccggttgca gcgggtgagg gcgctgacgg
     4861 tcttgctggc ctcgaccagg tgtaaaccat gatctttgcc gtgtgaaaac aagtacggcc
     4921 gtctcgtgac caggagaaag gctccgacag tggctccgac cttgctgtct ggtgtcgttg
     4981 cgtgtcggct gctgtcgctg gttgtagggg cttcgccgcc tgttcgccgc ctgccagtct
     5041 tggcgggttg tgtggttcgt aacgctttcg ggacgatgct gagg
//
LOCUS       DATADN010000089         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_312019, whole genome shotgun sequence.
ACCESSION   DATADN010000089 DATADN010000000
VERSION     DATADN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312019"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1176
                     /locus_tag="VHH53_01150"
     CDS             256..1176
                     /locus_tag="VHH53_01150"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2288820.1"
                     /translation="MTHQALLYGSEEEFLAATVPFVRDGLDRGDPIRVVTTSRNTGWL
                     RVALGADARHVAFCDSSQWYRHPVRTLAALHHTVQTAALDGQVLRMIEDPMCTARTSQ
                     ESKEWARYESLVNAALARASAALVCTYDTRVVESDVVAEVTRTHPELVVNGGSEPSPS
                     YVDPAVFNAETDKLPLPPAPPSALRLKFDGVNQLSTLRGFVISYVTWAGAAVQAAEQL
                     VQAVDEVATNAIDHGGGSGQLRVWTDPQWISCEVSDTGAGLRDPLAGRLPTGFTARGR
                     GLWLARQLCDLVELHSDPAGTTVRLHLALP"
     gene            complement(1248..>1539)
                     /locus_tag="VHH53_01155"
     CDS             complement(1248..>1539)
                     /locus_tag="VHH53_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2288821.1"
                     /translation="TVMGEVDALTAPELATFLTAQLTVAQVVVVHLDGVRFLGSAGLS
                     VLFEANDLATQEDRELRLVCNSRTANLALDATGLREHFTFADSVPDALNSSP"
BASE COUNT          266 a          476 c          523 g          274 t
ORIGIN      
        1 cgccaaccgg aacgtcgtcg ccgatccgga cctcatccgg gtagggcaaa cgctgcagat
       61 cccgctgtag tcccgcagcg accggctaac ctcggacgag ccccgaaggc caccccggag
      121 ctcgccgagc gggccgtcct gcaccggtgt ttggggaagt cggcagccgg gcatgcctgg
      181 tagtcagctt agggagcggt ggtgatgagt ggcggcgcgc tgagcagtgt cgataccgat
      241 gagagtggag ggtcattgac gcatcaggcc ctgttgtacg ggtctgagga ggagttcctc
      301 gcggccactg tgccgttcgt ccgagacggc ttggaccgcg gtgatcccat ccgagtcgta
      361 actaccagcc gtaataccgg ctggctgcgg gtagcgctgg gtgccgatgc ccgccatgtg
      421 gcgttctgtg atagcagcca gtggtaccgg catccggtgc ggacgttggc ggcactgcac
      481 cacaccgtgc agacagctgc actggacgga caggtgctac ggatgatcga agacccgatg
      541 tgcactgccc gtaccagtca agagagcaag gagtgggctc gatacgagtc acttgtcaac
      601 gcggcgttgg cacgggccag cgccgcgttg gtgtgcacct acgatactcg cgtcgtggaa
      661 tctgatgttg tcgcggaggt cacccggacc cacccggagc tggtggtcaa cggaggctcc
      721 gaacccagcc ctagctacgt cgatccggcg gtgttcaacg ctgaaaccga caagttgccc
      781 ttgccgccgg cgcccccttc agcgttgcgg ttgaaatttg acggagtgaa ccagctgtcc
      841 acgttgcgcg ggttcgtgat ctcctatgtc acctgggcgg gagcggctgt gcaagctgcc
      901 gagcagttag tgcaggcggt cgacgaggtc gcaaccaacg cgatcgatca cggcggtgga
      961 tcaggccaac tgcgggtctg gaccgatccg cagtggatat cgtgcgaagt cagcgatacc
     1021 ggtgccggac tgcgcgaccc cttggccggg cgcttgccta ccggcttcac cgcacgcgga
     1081 cgagggctgt ggttggcgcg acagttgtgc gatcttgtcg aactgcacag cgacccggcc
     1141 ggcacgaccg tacggctgca tctggccttg ccgtgacgct gtgccttgac ggctgctccg
     1201 gcacctaccg ccaggctgtc cccggtcgct ggcggttcgc gcgcctgtca gggtgagctg
     1261 ttcagagcat ccggcacgct gtcagcgaag gtgaaatgct cccgcaggcc cgtggcgtcc
     1321 agcgccaggt ttgcggtccg ggagttgcat accagccgca gttcacggtc ttcttgggta
     1381 gcaaggtcat tggcctcgaa gagcacggaa agcccggcag aacccaggaa ccgcacccca
     1441 tcgaggtgga ccaccacgac ttgggcgaca gttagctgcg cggtcaagaa ggtggccagt
     1501 tccggtgcgg tcagcgcgtc cacctcgccc atcacagtg
//
LOCUS       DATADN010000090         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_161406, whole genome shotgun sequence.
ACCESSION   DATADN010000090 DATADN010000000
VERSION     DATADN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161406"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1502
                     /locus_tag="VHH53_01160"
     CDS             <1..>1502
                     /locus_tag="VHH53_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX2288822.1"
                     /translation="LAGYWANVLAAVDVVTEVASNRWDPAVYSEPAKWGGFLPDIPFD
                     ALAYGIPPAALASIEPVQLLALEVAAQALRDAGYARRAFDREHTSVIFGAEAGTDLAS
                     AYGFRSLYPAYHGTLPPELDEQLPKLTEDSFPGVLANVIAGRIANRLDLGGANYTVDA
                     ACASALAAIDLACKELRAGTSKMVLVGGADLHNSIHDYLMFASVQALSPTGRCRPFDA
                     SADGIALGEGVGCVVLKRLADAERDGDRVYAVIKGIGSASDGRSLGLTAPRPDGQRRA
                     LERAYRMAGISPADVGLVEAHGTGTVVGDRTELAVLTELFTAAGTETGSCTLGSVKSQ
                     IGHTKCAAGIAGLIKSACAVHTGVRPPTGQLRNPNPYWDCATSPFSFDLAPRPWTSSA
                     PRVAGVSAFGFGGTNFHAVLTSYGGAPEPRHGLDAWPAELFLFTGADRVAAIAELDRV
                     IELLAANDSAGRPWRLRDLARTVCGSRRGRIWTAVVADDLDDLATKLTRAR"
BASE COUNT          221 a          507 c          524 g          250 t
ORIGIN      
        1 ctcgccggct attgggccaa cgtgctggct gcagtggacg tggtcactga ggtggcttcc
       61 aaccggtggg atccggccgt atacagcgag cccgcgaagt ggggtggctt cctcccggac
      121 atcccgttcg acgcactggc ctacggcatc cccccggctg cgctggccag cattgaaccg
      181 gtccagctgc tggcgctgga agtggccgcc caggcgctgc gcgacgctgg gtacgcgcgg
      241 cgagccttcg atcgggagca cacgtcggtg atcttcggcg ccgaggcggg caccgacctg
      301 gccagcgcgt acggtttccg ttcgctctac ccggcctacc acgggacgct gccgccggag
      361 ctggacgagc aactaccgaa gctgactgag gactcgttcc ccggagtgct ggccaacgtg
      421 atcgccggcc ggatcgccaa ccggctggat cttggtggcg cgaactacac ggtggatgcc
      481 gcctgcgcct ccgcgctggc cgccatcgac ctggcctgca aggagctgcg cgccggtacc
      541 agcaagatgg tgctggttgg tggcgccgac ctgcacaaca gcatccacga ctacctgatg
      601 ttcgcctccg tgcaggcgtt gtcgccgacc ggccggtgcc gtccgttcga cgcctccgcc
      661 gacggcatcg cgctcggcga aggtgtggga tgcgtcgtgc tcaagcggct ggccgatgct
      721 gaacgcgacg gcgaccgggt ctatgcggtg atcaagggaa tcggttcggc cagcgacggt
      781 cggtcgctgg gcctgaccgc accgcggccc gacggccagc gtcgcgcgct ggagcgcgcc
      841 taccggatgg ccgggatctc gccggccgac gtcggactgg tcgaggcgca cggtaccggc
      901 actgtggtcg gggaccgcac ggaactcgcg gtgctgaccg agctgttcac tgcggctggc
      961 accgagaccg gcagctgcac gctgggctcg gtgaaatccc agatcgggca taccaagtgc
     1021 gctgcaggga tagccgggtt gatcaagtcc gcgtgcgcgg tgcacaccgg tgtgcggcca
     1081 ccaactggcc aactgcggaa tccaaacccg tactgggact gcgccaccag tccgttctcc
     1141 ttcgatctcg cgccgcgacc ctggacgtct tcggcgcctc gagtcgccgg cgtaagtgcg
     1201 tttggattcg gcggcactaa cttccacgcc gtactgactt cttacggcgg tgcacccgag
     1261 ccacgacacg gattggacgc gtggcccgcc gagctgttcc tgttcaccgg tgccgatcgg
     1321 gtggcggcga tcgcggagct ggaccgggtg atcgagctgc tcgcggccaa tgactccgct
     1381 ggtcgaccct ggcggctgcg ggacctcgcc cggaccgtgt gcggttcccg ccgtggtcgg
     1441 atatggaccg cagtggtggc cgacgacctt gacgacctgg caacaaagct aacccgggct
     1501 cg
//
LOCUS       DATADN010000091         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1066113, whole genome shotgun sequence.
ACCESSION   DATADN010000091 DATADN010000000
VERSION     DATADN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066113"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHH53_01165"
     CDS             <1..198
                     /locus_tag="VHH53_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEX2288823.1"
                     /translation="RPDGTTARFLVGRLERHPKTALPTSRIWTKANRPLLRLITCAGS
                     FDRTTGHYRDNLIVYASPSGS"
     gene            complement(215..487)
                     /locus_tag="VHH53_01170"
     CDS             complement(215..487)
                     /locus_tag="VHH53_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288824.1"
                     /translation="MPGWRSASGEIRPAARPRSSSSGSGGWPLSRSGDGWRGQSSIGP
                     VRGAIEIAGEDTVREAFSDAVAQFRQPDGSYRQENVFRYVIAGKPA"
     gene            complement(964..1629)
                     /locus_tag="VHH53_01175"
     CDS             complement(964..1629)
                     /locus_tag="VHH53_01175"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX2288825.1"
                     /translation="MAGQDHTAAPQASGASAATRAAAAAIAGVCAGALAALPGSWQIA
                     TLAGWDVAAVVYVAWTWATIWHRDPAATAHLALREDPGRAIADALLLVASVASILAVA
                     LAISAGSTGGPGERDLRAGLAVASVALSWAVVQTVFTSRYARLYYSHPAGGIDFNQDA
                     SPRYSDFAYLAFTVGMTFQVSDTDLQTSALRAEALRHALLSYLLGAVILATTINLVAG
                     LLR"
BASE COUNT          259 a          670 c          624 g          255 t
ORIGIN      
        1 cggcccgacg ggaccaccgc ccggttcctg gtcggccgcc tggagcggca ccccaagacc
       61 gccctgccga ccagccggat ctggaccaag gcaaaccggc ccctgctccg cctgatcacc
      121 tgcgccggca gcttcgaccg aaccaccggc cactaccgcg acaacctgat cgtctacgcc
      181 tcgcccagcg gctcctgagg cagcgtggcc acgctcaggc cggcttgccg gcgatcacgt
      241 accggaacac gttctcctgc cggtacgagc cgtccggctg ccggaactgc gccactgcgt
      301 cggagaacgc ttcccggacg gtgtcttcgc cggcgatctc gatggcgccg cggaccgggc
      361 cgatcgacga ctgtccccgc cacccgtctc cagatcggga taggggccaa cctccggacc
      421 cgctggaaga cgacctcggt ctcgcagcgg gccggatctc cccggacgcc gatcgccacc
      481 cgggcaccgg gccgggtcac cggggccagc tccgccgctg ccgccttcgg ctcggcggca
      541 tactgcacgc gttgcacgcc gtcaccacgt cgaaggtcgc gtcgccgaac ggcagctact
      601 gggatgtcac cgacccgcag gtccgcatcg ggacgtcgct cgcgggcgat ggcgagcatc
      661 ggcgcggccg cgtccactgc tcgcacacct cgggaccagg ttgctgttgc gggtggcgaa
      721 cgtcaccacc actgtggcga cccacagccc caggccgccc aggaacatgt gcagccaggg
      781 tcgcctgctc caccagctgt gggtcctggt ccgagtccga gcggtggccg gaaccacttc
      841 cggcgttgtc tgtgtcatgg ctcccctcct tgtgctggcc caagggtcgc gccacgaccg
      901 gccggtgtct tcaccgccag cggccatctg ctctggcggc tggctggaat ccggggcctc
      961 ccgctacctg agcagtcccg ccaccaggtt gatggtggtg gccaggatga ccgcgcccag
     1021 caggtatgac agcagcgcgt gacgcagcgc ctccgcccgc agggccgagg tctggaggtc
     1081 ggtgtcggac acctggaagg tcatcccgac ggtgaaggcc aggtaggcga agtcggagta
     1141 ccggggcgac gcgtcctggt tgaagtcgat cccgccggcc ggatggctgt agtacagccg
     1201 ggcgtagcgt gaggtgaaca ccgtctgcac caccgcccat gagagggcca cgctggccac
     1261 cgccagcccg gcgcgcagat cccgctcgcc ggggccaccg gtactgccgg cgctgatggc
     1321 cagggcgacg gccaggatgc tggccacgct ggcgaccagc agcagggcgt cggcgatggc
     1381 tcgtcccggg tcctcacgca gggccaggtg ggcggtcgcg gccggatccc ggtgccagat
     1441 cgtcgcccac gtccaggcca catacaccac ggcggcaacg tcccacccgg ccagggtggc
     1501 gatctgccag ctgcccggca gcgcggccag cgcgcccgcg cagaccccgg cgatggcggc
     1561 tgccgccgcg cgggtggccg cggaggcccc cgacgcctgc ggtgcggctg tgtggtcctg
     1621 gccagccaac ggatcagtcc tttcgggtcc ttcacccgtg ctggatgacc ggtgaggctc
     1681 gacggtcagg gtaagcatct cgccgggaac ggtcgcctgt cttcccggcc gcgcataccg
     1741 gctagctgga aactcatggg cctgaggggt gggtggccag gtcgcggttc gggcagtgga
     1801 ccacctcc
//
LOCUS       DATADN010000092         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_463247, whole genome shotgun sequence.
ACCESSION   DATADN010000092 DATADN010000000
VERSION     DATADN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463247"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VHH53_01180"
     CDS             complement(<1..461)
                     /locus_tag="VHH53_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288826.1"
                     /translation="MALALKRTVPKVSLGTLFVAVQLADLLWGGFLLLGWERAQVVPG
                     YTPVTPLEFLSYPISHSLVAAVLWGIVAAALYYSWPTRDTARHWQAAALVGVAVFSHY
                     PLDVLVHVPDLPLAGDDSPKLGLGLWNYPLAAFVAELLVLTAGLVVYVLFRS"
     gene            complement(490..1329)
                     /locus_tag="VHH53_01185"
     CDS             complement(490..1329)
                     /locus_tag="VHH53_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2288827.1"
                     /translation="MSAAESVFRPDLLAGKVAVITGGGTGIGLGISRSLAAAGAQVAI
                     ASRKPEHLEPAAEQLRATGARVSAVEVNVREPESVARMVQQVSREHGRLDILVNNAAG
                     NFYAPSATLSPNGWRAVVETDLYGTFYCCQSVYPIMKAQGSGRIVSISMTLHYRGWPL
                     MAHATAAKAGVDALTRTLALEWARDRITVNAVAPGPIPTEGVKKAFTPPGAEAPDLFG
                     MEQYAAATIPLGRWGTPEEIGQMVTYLASPAGDWITGAIMVVDGGAWLGGGEAGKPGR
                     GEG"
     gene            complement(1326..>1642)
                     /locus_tag="VHH53_01190"
     CDS             complement(1326..>1642)
                     /locus_tag="VHH53_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288828.1"
                     /translation="RAMRVLTTDQSIDAQTALSIGLVEEVIEDSELDAGVTRVVSALV
                     SAAGPSVRAIKRLSRAQEVGALEQALSSEGAAQLQALQGPEFRSRLEAFTARVSTPKA
                     ST"
BASE COUNT          298 a          548 c          519 g          277 t
ORIGIN      
        1 gatcggaaca acacgtagac gaccaatccc gcagtcagga cgagaagctc cgccacgaag
       61 gctgcgagtg gatagttcca gagccccaga cccagtttcg gggaatcgtc tccggcgagc
      121 ggcaggtctg gaacgtgcac cagcacatcc agcggatagt gggaaaacac ggcaacgccc
      181 accagagcgg cagcctgcca gtggcgtgcg gtgtctcgcg ttggccagga gtagtagagg
      241 gctgccgcaa cgatgcccca gagtaccgcg gcaaccagac tgtgcgaaat ggggtaggag
      301 agaaactcga gcggggtgac aggcgtgtag ccgggaacga cttgcgcccg ctcccagccc
      361 agcagcagaa acccgcccca gagcaggtcg gccagctgga ccgcaacgaa cagtgtcccg
      421 agggacacct tgggcactgt gcgcttgagc gcgagcgcca caccgtagtg acctaggaac
      481 acggccgctt cagccttccc cgcgccccgg cttccctgcc tccccgcctc ccagccacgc
      541 tccgccgtcc accaccatga tcgctccggt gatccagtct ccggcgggac tggcgagata
      601 ggtgaccatc tgcccgatct cctctggagt tccccagcgg cccagaggaa tggtggctgc
      661 ggcgtactgc tccatcccaa acaggtcggg ggcttcggca cctggaggag tgaatgcttt
      721 ctttacgcct tccgtgggaa tgggtccagg tgccacggca ttgaccgtga ttcgatcccg
      781 ggcccattcc agcgcgagtg tacgggtcag cgcatccacg ccggctttgg ccgctgtggc
      841 atgggccatg agcggccagc ctcgatagtg caatgtcatg gatatggaga cgatccggcc
      901 tgaaccctgc gccttcatga tggggtagac cgactggcag cagtaaaaag ttccgtagag
      961 gtctgtctcc accaccgcgc gccagccatt tggtgacagc gtagcgctgg gcgcgtagaa
     1021 gtttcccgcg gcattgttga cgaggatgtc cagccggccg tgttcgcggg acacctgctg
     1081 caccattcgg gcgacagatt cgggctcgcg cacgttcacc tcgacggcac tgaccctggc
     1141 acctgtggcc cgcaactgtt ctgctgccgg ctcgaggtgc tcgggcttcc ggctggcaat
     1201 ggcgacttga gcccccgcag cagccaggct ccggctgata cccagcccaa tgccggtgcc
     1261 gcctccggta atcaccgcca ccttgccggc gaggaggtcc ggccgaaaca ccgattctgc
     1321 cgcgctcacg tgcttgcttt cggggttgag actcgcgcgg tgaacgcttc cagccgggaa
     1381 cgaaactccg gaccctgcag cgcctggagc tgggccgcgc cttcgcttga aagtgcctgc
     1441 tccagggctc ccacctcctg cgctcgggac agccgcttga tggcccgtac actgggtccg
     1501 gcggcggaaa ccagggcgct gacgacgcgg gtcacgccgg cgtccagctc actgtcttcg
     1561 atgacttcct ccaccaaccc gattgaaagg gcagtctgcg catcgatcga ttggtcggtt
     1621 gtcagcaccc gcatggcacg gc
//
LOCUS       DATADN010000093         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_10795, whole genome shotgun sequence.
ACCESSION   DATADN010000093 DATADN010000000
VERSION     DATADN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10795"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VHH53_01195"
     CDS             complement(<1..376)
                     /locus_tag="VHH53_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HEX2288829.1"
                     /translation="MKAVALGFLLGAAVVYVVARLYEQPGGATWIGYVRAMAEASMVG
                     ALADWFAVTALFRHPLGLPIPHTAIIPRKKDALGGNLGDFVGVNFLAEEVVIDKLRRV
                     GISARFGRWLAQPRHAERVTAEL"
     gene            477..1664
                     /locus_tag="VHH53_01200"
     CDS             477..1664
                     /locus_tag="VHH53_01200"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2288830.1"
                     /translation="MRTHGLVPRLQPFTSTIFAEMTSLALRTSAVNLGQGFPDTDGPA
                     GMLEIARSAIASGCNQYPPGSGIPELRSAIAEHQAAHYGLMHDPDTEVLVTVGATEAI
                     SAALLALVHPGDEVLLIEPYYDSYAAAVALAGATRHSVPLVADGDRFALDPDALAEAI
                     TPATRALILNSPHNPTGTVLHRAEVEALAALATEHDLVVITDEVYEHLVFDGAQHFPI
                     STLPGMAQRTLSISSAGKTFSVTGWKIGWVCGPAHLVAAVRATKQFLTFVGGAPFQPA
                     VAHALRTEQQWVAAHRAALQAKQHRLAAGLGAAGFGVHRSEGTYFVCADVRPLGYSDG
                     LQFCRQLPDRAGVVAVPVQAFVDDPAPWRHLVRFAFCKRDEVLDEAIARLASARLSQS
                     PNR"
     gene            1823..>2566
                     /locus_tag="VHH53_01205"
     CDS             1823..>2566
                     /locus_tag="VHH53_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288831.1"
                     /translation="MAAIPDAGGARCAPRGVLRALPWAALLRVAVLGGLVIVGWLLGS
                     GIGQAQEELVPQDLGQQDNIVQLAGFPLPDNDSQGPLGAAPTVKSAVKGVVHAVPVPR
                     LPIQQVPVLAPVLAPVSTLAAQATVIRPHTQSQPTTKQVMVVPPPAAPAEPTVRAATV
                     VPPSPMHTVHSASPAAVACIVADPVADPLTDQIADPSVEQFPAPGSGPTAPAPASPFG
                     TSTAPCPSGSTGSGGVNGSAHPVAISDGVS"
BASE COUNT          407 a          898 c          848 g          413 t
ORIGIN      
        1 acagttccgc ggtgactcgc tcggcgtggc ggggctgtgc cagccaccga ccaaaccgag
       61 cgctgatccc gacccggcgt agcttgtcga tcaccacctc ttcggcgaga aagttgacac
      121 ccacgaaatc acccaggttg cctcccagcg cgtccttctt gcgggggatg atcgccgtgt
      181 gcgggatcgg caacccgagc gggtggcgga acagcgcggt gacggcaaac cagtcggcca
      241 gtgcaccgac catgctggcc tcggccatcg cccggacata cccgatccac gtcgcgccgc
      301 cgggttgctc gtagagccgc gccaccacgt acacaaccgc ggcacccagc aggaagccca
      361 gggcgaccgc cttcatccgg cggaggtccc gctgtttttc ctgctcgttt atcgtcaact
      421 ccacgctgcc gattgtgcct gcctccgcgc gctatgggcc cggcaggata agagctgtgc
      481 gaactcacgg actggtcccc cggctgcagc cgttcacatc gacgatcttc gcggagatga
      541 cctcgctggc cttgcgcacc agcgcggtga acctcggcca gggcttcccg gacaccgatg
      601 gcccggccgg catgctcgag atcgcccgct cggcgatcgc cagcgggtgc aaccagtacc
      661 cgcccggatc gggaatcccc gagctgcgca gcgcgatcgc cgagcatcaa gcagcgcatt
      721 acggcctcat gcatgatccg gacactgagg tgctggtgac cgtcggcgcc accgaagcga
      781 tcagcgccgc gctgctggcc ctggtgcatc cgggcgacga ggtgctgctc atcgagcctt
      841 actacgactc ctacgctgcc gcggtcgcgc tggctggtgc gacccgccac agcgtgccgc
      901 tggtcgccga cggtgaccgg ttcgccctag atccggatgc gctggccgag gcgatcacgc
      961 cggctacccg agcgctgatc ctcaacagcc cgcacaaccc caccggcacg gtgctgcacc
     1021 gcgccgaggt cgaggcactg gcggccctgg ccactgaaca cgacctggtg gtgatcaccg
     1081 acgaggtgta cgagcacctg gtcttcgacg gtgcacagca cttcccgatc tcgaccttgc
     1141 cggggatggc gcaacgcacg ctgtccattt ccagtgctgg caagacattc agtgtcaccg
     1201 gctggaagat cggctgggtg tgcgggccgg cacatctggt ggccgcggtg cgggcgacca
     1261 agcagttcct caccttcgtc ggcggggcgc catttcagcc cgcggtggcc cacgccctgc
     1321 gcaccgagca gcagtgggtc gcggcgcatc gcgcggcgtt acaagccaag cagcaccggc
     1381 tggccgcggg gctgggtgcg gccgggttcg gcgtgcaccg cagcgagggc acctacttcg
     1441 tctgtgcgga cgtccgaccg ctcggttact ccgacggcct ccagttctgc cgtcagctgc
     1501 ccgaccgggc cggagtggtg gcagtgccgg tgcaggcatt cgtcgatgac ccagcgccgt
     1561 ggcggcacct ggtgcgcttc gcgttctgca aacgcgacga ggtgctcgat gaggcaatcg
     1621 ctcgcctcgc ctcggcgcgc ctgtctcagt cgccaaatcg gtgacgatcg ctacagattc
     1681 ccatataccg gtcacagcgt gtagtgtgag gtagcgattg tccgagtgac ggagtctaga
     1741 cttctcgccc ggctggagat caggacgttg gcgcggcaag ccggcgcagg gccggtcccg
     1801 cggaagggtt gagggggaac cggtggccgc catccccgac gcaggtggcg cacggtgtgc
     1861 gcctcgcggg gtgctgcgtg cactgccatg ggctgcactg ctgcgggtcg ctgttctcgg
     1921 ggggctggtc atcgtggggt ggctgctggg atccggaata gggcaggcac aggaagaact
     1981 ggttccccag gacttgggac agcaggacaa catagtccag ttggccggat tcccgttacc
     2041 ggataacgac tcccaaggtc cgctcggtgc ggcacccacg gtgaagtcgg cggtcaaggg
     2101 cgtggtgcat gccgtgccgg taccgcggct tcccatccag caggtccccg tgctggctcc
     2161 cgtgctcgcc ccggtgtcga cgctggctgc ccaggccacg gtgatccggc cgcacaccca
     2221 gtcacagccg acgaccaagc aggtaatggt cgtgccaccg ccggccgcgc cagctgagcc
     2281 gaccgtccgg gcagcgaccg tcgtcccccc ttccccgatg cacacggtgc acagcgcctc
     2341 acccgcggct gtggcctgca tcgtcgctga tccggttgcc gatccgttga cggaccagat
     2401 cgccgatcca tcggtagagc aattcccagc accgggcagc ggcccaacgg ccccggctcc
     2461 cgcaagtcca ttcggaacca gcaccgcgcc atgcccatcc ggtagcaccg gaagcggggg
     2521 cgtcaatggc agtgcccacc ccgtcgccat aagtgacgga gtatcc
//
LOCUS       DATADN010000094         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_915406, whole genome shotgun sequence.
ACCESSION   DATADN010000094 DATADN010000000
VERSION     DATADN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915406"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VHH53_01210"
     CDS             complement(<1..302)
                     /locus_tag="VHH53_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2288832.1"
                     /translation="MVRRSGRRPGNQDTRQSILDAARAMFAERGFDGASIRAIATEAK
                     VDPALVHHYFGTKDKLFLESMNMPLNPAELVPKALSGPREEVGERLVRLVLSVWDSP"
     gene            complement(305..1042)
                     /locus_tag="VHH53_01215"
     CDS             complement(305..1042)
                     /locus_tag="VHH53_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2288833.1"
                     /translation="MSARITFATCARILRQLRHDHRTVALLVAVPSLLLTLLFFMYDN
                     AGPTFDRIALIMLGVFPFVIMFLVTSIAMLRERTTGTLERLLTTPLHKLDLLFGYGIA
                     FGLAAAVQGAVAVGVSFWLLGLETMGSAGLVVLIAVVNAVLGVALGLFCSAFARTEFQ
                     AVQFLPVVVVPQLLLCGLFVPRGQMSGWLQAASDVMPLSYAVEALAQVGTNADPTTTM
                     WRDLAVVAGAAVVALVLAAATLRRRTP"
     gene            complement(1042..1764)
                     /locus_tag="VHH53_01220"
     CDS             complement(1042..1764)
                     /locus_tag="VHH53_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2288834.1"
                     /translation="MDNAIEVRDLVVDRGKRRVLHGISCAVPTGSVTGLLGPSGSGKT
                     TLIRAVVGVQIVRSGTVTVLGLPAGSAALRRTVGYQTQAPSVYADLSVRENARYFATL
                     YGLDARAADEAVRDVGLADAAGQLVGNLSGGQRSRASLACAMIGRPGLLVLDEPTVGQ
                     DPVLRADLWAKFHALAAEGTTLLVSSHVMDEAGRCDRLLLIREGRLINDDTPAAIRDR
                     ADTDDLEEAFLRLIREQERVAA"
BASE COUNT          296 a          677 c          626 g          252 t
ORIGIN      
        1 ggtgagtccc acacgctcag caccagccgc accagccgct cgccgacctc ctcgcgcggc
       61 ccggacagcg ccttgggcac cagctcggcc gggttcagcg gcatgttcat gctctccagg
      121 aagagcttgt ccttggtgcc gaagtaatgg tggaccagcg ccgggtcgac cttcgcctcg
      181 gtcgcgatgg cccggatcga cgccccgtcg aagccccgct cggcgaacat cgcacgggcg
      241 gcgtccagga tggattgccg ggtgtcctgg ttgccggggc gccgcccgct gcgtcgtacc
      301 aagatcaggg tgtccttcgt ctcaacgtcg ccgcggccag gacgagcgcg accaccgccg
      361 cacccgccac cacggcgagg tcccgccaca tcgtcgtggt ggggtcggcg ttggtgccga
      421 cctgggccag cgcctccacc gcgtaggaca acggcatcac gtcgctcgcc gcctgcagcc
      481 agccggacat ctgcccgcgc ggcacgaaca gcccgcacag cagcagttgc ggcacgacca
      541 ccaccggtag gaactgtacg gcctggaact cggtgcgggc gaaggcgctg cagaacaggc
      601 cgagggccac acccaggacg gcgttcacca ccgcgatcag caccaccagt ccggcgctgc
      661 ccatggtctc cagcccgagc agccagaacg agacgcccac cgcgaccgca ccctgcacgg
      721 cggccgccag cccgaacgcg atcccgtacc cgaacagcag gtccagcttg tgcaacgggg
      781 tggtgagcag gcgttccagg gtgccggtgg tgcgctcgcg cagcatggcg atgctggtca
      841 ccaggaacat gatcacgaac gggaacacgc cgagcatgat cagcgcgatc cggtcgaagg
      901 tcggtcccgc gttgtcgtac atgaagaaca gcagggtcaa cagcagtgac ggtacggcga
      961 ccagcagcgc caccgtccgg tggtcgtgcc ggagctgccg caggatccgc gcgcacgtgg
     1021 caaaagtgat ccgggcactc atcaggcggc caccctctcc tgctcgcgga tcaggcgcag
     1081 gaacgcctcc tccaggtcgt cggtatcggc ccggtcccgg atcgcggccg gggtgtcgtc
     1141 gttgatcagc cggccctccc ggatgagcag cagccggtcg cagcgacccg cctcatccat
     1201 cacgtgactg gacaccagca gcgtggtgcc ctcggcggcc agcgcgtgga acttcgccca
     1261 caggtcggca cgcagcaccg ggtcctggcc gacggtcggc tcgtccagca ccagcaggcc
     1321 cgggcggccg atcatcgcgc aggccagcga ggcccggctg cgctggccgc cggagaggtt
     1381 cccgaccagc tggccggccg cgtccgccag cccgacgtcc cggacggctt cgtcggcggc
     1441 ccgcgcgtcc agcccgtaca gggtggcgaa gtagcgggcg ttctcccgga ccgacaggtc
     1501 ggcgtacacg ctcggcgcct gggtctggta tcccaccgtc cgccgcaggg cggcgctgcc
     1561 ggccgggagg ccgagcaccg tgacggtgcc ggaccggacg atctgcacgc cgacgacggc
     1621 ccggatgagg gtggtcttgc cgctgccgct ggggccgagc aggccggtca cgctgccggt
     1681 gggcaccgca cagctgatgc cgtgcagcac ccgtctcttc ccccggtcga ccacgaggtc
     1741 gcggacctcg atggcgttgt ccatgcttgc ctccttagta tttcaccacg cgttgagttc
     1801 aacagtaggt gaattccgcg gaccgcacaa gaacgagacg gccgtcgccc c
//
LOCUS       DATADN010000095         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_161589, whole genome shotgun sequence.
ACCESSION   DATADN010000095 DATADN010000000
VERSION     DATADN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161589"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="VHH53_01225"
     CDS             complement(<1..130)
                     /locus_tag="VHH53_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEX2288835.1"
                     /translation="MRILVLGGDGYLGWPTALHLSDRGHDVAVLDNFARRAYDDELG"
     gene            226..558
                     /locus_tag="VHH53_01230"
                     /pseudo
     CDS             226..558
                     /locus_tag="VHH53_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
     gene            complement(624..>1716)
                     /locus_tag="VHH53_01235"
     CDS             complement(624..>1716)
                     /locus_tag="VHH53_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2288836.1"
                     /translation="LALVAGLLGGLGVGNARRSGIRAASLLVSGLGITVLMTAAAFEF
                     SWRYQLPGLVLLPMAGVLGITALTGPLRRPGPRLKLRPPMQPYPDPVDTATVREFTQA
                     EGEVRFAPVVVIIAAYDEEEGIGAVLDAVPSASCGLPVDTLVIVDGCTDGTAEVARRH
                     GARVCQATINRGQGAALRLGYRLARRGGARYVVTTDADGQYDLDELPLLLQPLIDDEA
                     DFVTGSRVLGRQETSDPVRHLGVRVFAALARALTRQRLTDTSFGFRATNAEVTDAVTL
                     AQAQYQASELLLGVISHGFRVVEQPMTMRARAAGETKKGNNLVYGMRYARVMVGTWWR
                     ERSIRRSVAWSRPKTSRSSSANLSMKSGR"
BASE COUNT          271 a          615 c          563 g          267 t
ORIGIN      
        1 ggcccaactc gtcgtcgtag gcacggcggg cgaagttgtc cagcacggca acgtcgtggc
       61 cccggtccga gaggtgcagc gcagtcggcc agccgaggta accgtcaccg ccgagaacga
      121 ggatccgcac cgtgttgcct cctgccgggg taaccggggt tagtggacgc atttgcctat
      181 cagatggacg gcgcgagcgc caacagcggc tacctctgga cgccgatgcc ggtctgcgaa
      241 gatgcagagg tgctccgacg gctcgccagt tcgccactag cgcggttgtt cacccggttc
      301 gccgcgggca cggcagtgtc caccgcatgc agccagctgg cgttcgtgct gctcttcggg
      361 gttgcagcgg cctcggccat ggtctccggg atggtggcct tcgtcgccgg tgtggtgccg
      421 aacttcctta tccaccggtt ctggacgtgg cgtcgcggcg gcccagtgct gatacgccgg
      481 gaactggtgc cctacctggc ggtgatcatt gtcagcggcc tggtagcgat cggtatgacc
      541 accagcgtcg accggttgat cggccagacc atcgatgacc gcgcgttacg cactctcgtc
      601 gtcgcggtgg ccttcaacgc cagctacctg ccgctcttca tactcaagtt cgcgctgctg
      661 gaccggctgg tctttggccg tgaccaagcc acgctgcggc ggatcgagcg ctcccgccac
      721 caggtgccca ccatcacccg cgcgtagcgc atcccataca ccaagttgtt gcccttcttg
      781 gtctcacccg cagcgcgggc ccgcatcgtc atcggctgct ccacgacccg gaaaccgtgc
      841 gagatcaccc ccaacagcag ctccgaggcc tgatactgcg cctgcgccag cgtgacagca
      901 tcggtcactt cagcattcgt cgcgcgaaag ccgaacgagg tgtcagtcag ccgctggcgg
      961 gtcagcgcgc gggccaaggc ggcgaacacc cgcaccccga ggtgtcgcac cgggtcggag
     1021 gtctcctggc gccccagcac ccgcgagccg gtgacgaagt cggcctcatc atcgatcaac
     1081 ggctgcagca gcaggggcaa ctcgtccagg tcgtactgcc cgtcggcgtc ggtggtgacc
     1141 acgtagcggg cgccaccacg acgggccaac cggtaaccca gccgcagtgc cgcgccctgg
     1201 ccccggttga tcgtcgcctg gcagactcgc gcgccgtgcc ggcgggccac ctcggcggtg
     1261 ccgtcggtgc acccgtcgac gatcaccaag gtgtccaccg gcagaccgca gctcgccgac
     1321 ggcaccgcgt cgagcacggc gccgatacct tcctcctcat cgtaggccgc gatgatcacc
     1381 acaaccgggg caaaccgcac ctcgccctcg gcctgggtga actcccgcac cgtggcggtg
     1441 tccaccggat cggggtaggg ctgcatcgga ggtctcagct tcagccgggg gcctggcctg
     1501 cgcagtggtc cggtcagcgc agtgatcccc aggactccgg ccatcggcag cagtaccagc
     1561 ccgggcagct ggtagcgcca ggagaactcg aaggccgccg cggtcatcag caccgtgatc
     1621 ccgagtccgg acaccagcag gctggcagcg cggatccccg accggcgggc gttgccgacg
     1681 ccgagaccgc cgagcagtcc ggcgaccagc gccagg
//
LOCUS       DATADN010000096         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_614284, whole genome shotgun sequence.
ACCESSION   DATADN010000096 DATADN010000000
VERSION     DATADN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614284"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VHH53_01240"
     CDS             complement(<1..482)
                     /locus_tag="VHH53_01240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2288837.1"
                     /translation="MSTVIAVVVTRHRRDLLAKSLAVLSEQTRPPDHLVVVDNGPDDP
                     AEGVVEDCSIPSTYLPSLRNLGGAGGFALGMLHALALGAEWVWLADDDGRPADVRVLQ
                     TLLEEAKRRDLAVISPVVVNIDDPERLAFPLRRGLTWKRQRSELGDDFLPGIASFFNG
                     A"
     gene            complement(568..1368)
                     /locus_tag="VHH53_01245"
     CDS             complement(568..1368)
                     /locus_tag="VHH53_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2288838.1"
                     /translation="MVSIDVNGACVDFPIFDAKTRSLKKALLGTMGRAGGKIGIDSKV
                     PIIEALRNITLSLRNGDRVGLIGHNGAGKSTLLRLLSGIYEPTRGSSRIVGKVAPVFD
                     LGVGMDPEISGYENIIIRGLFLGMTRKQMEARTGEIAEFTELGDYLSMPLRTYSTGMR
                     VRLALGVVTSIDPEILLLDEGIGAVDSAFLEKARDRLTALVERSGILVFASHSDEFLI
                     ELCTSALWLDHGEIREQGDLRKVLSHYKGKDPYENLSQEALNRIGATR"
     gene            complement(1376..2248)
                     /locus_tag="VHH53_01250"
     CDS             complement(1376..2248)
                     /locus_tag="VHH53_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2288839.1"
                     /translation="MQHTATAAEISEAQAPPSRSWPRAVGDLAAGWRQRQLWGHLGWQ
                     DIKQGYRRSVIGPLWITISLAVQAIGMGILYAALFGQPLHDFLPYLAVGLIIWQFLAA
                     CINDGAEVFIRNEGLIKHLPAPLSVHIFRLVWRQLLFFGHNVLVWVALMLIFPPQAPR
                     AFGWSSLLAVPALALIVLNGVWVAILIGIIATRYRDVPPVTASIVTLMFFMTPIVWDY
                     EQLRNTPGTVGERARWVELNPFLHYVEILRRPMIGESFELRHWVVTLSITVVGWAVAL
                     LVLRNYRARVSYWV"
BASE COUNT          411 a          778 c          749 g          382 t
ORIGIN      
        1 gcgccgttga agaagctggc gataccgggc aggaagtcat ctcccagctc gctgcgctgc
       61 cgcttccagg tcaggccccg gcgcagcggg aacgccagcc gttccgggtc gtcgatgttg
      121 acgaccaccg gagagatcac cgcgaggtct cgccgctttg cctcttcgag cagggtttgc
      181 agcacccgga cgtccgcggg gcgcccgtcg tcgtccgcca gccacaccca ttccgcaccc
      241 agcgccaggg cgtgcagcat gcccagtgcg aacccgccgg cgccaccgag gttgcgcagc
      301 gagggcaggt aggtggacgg gatcgagcag tcctcgacga ccccctcggc gggatcatcc
      361 ggcccgttgt cgaccaccac cagatggtcc ggcggccgag tctgctcgct cagcaccgcc
      421 aacgacttgg ctagcaaatc ccgccgatgt cgcgtcacga cgaccgcgat gaccgtactc
      481 atcggtcacc catcgcatcc agcgggctca gcgtggtaaa aaccgggcca ataaccccgc
      541 tgagcccgct gaatgcggta tgggcggtca tcgggttgct ccgatccggt tgagggcttc
      601 ctggctgagg ttttcgtagg ggtccttgcc cttgtagtgg gaaagcacct tgcgtaggtc
      661 accctgctcc cggatctccc cgtggtccag ccacaacgcc gaggtgcaca gctcgatcag
      721 aaactcgtcg gagtgcgacg cgaacaccag gatcccggac cgctcgacca gcgcggtgag
      781 ccggtcccgg gccttctcca ggaatgccga gtccaccgcg ccgatgccct cgtcgagcag
      841 caggatctcc gggtcgatgc tggtcaccac accgagcgcg aggcggaccc gcatgccggt
      901 cgagtaggtg cgcagcggca tcgacaggta gtcgcccagt tcggtgaact cggcgatctc
      961 cccggtgcgg gcctccatct gcttgcgggt catgcccagg aacagcccgc ggatgatgat
     1021 gttctcgtag ccggagatct ccggatccat gcccacgccg aggtcgaaga ccggggcgac
     1081 ctttcccacg atccgcgagc tgcccctggt cggctcgtag atcccggaca gcagccgcag
     1141 cagggtggac ttgcccgccc cgttatgccc gatcaggccg acccggtcgc cgttgcgcaa
     1201 cgacagggtg atgttgcgga gtgcttcgat gatcggcacc ttggagtcga tcccgatctt
     1261 gccgccggct cgccccatcg tgcccagcag agctttcttc agcgacctgg tcttcgcgtc
     1321 gaagatcgga aagtcgacgc aggcgccgtt cacgtcgatg gagaccattg tgctatcaca
     1381 cccagtagga gactcgggcc cggtagttgc gcaggacgag cagcgctacc gcccacccca
     1441 ccacagtgat ggacagggtc accacccagt gccggagttc gaaggactca ccgatcatcg
     1501 ggcgtcgcag gatctcgacg tagtgcagga acgggttgag ttcgacccac cgggctcgct
     1561 ccccgaccgt gccgggggtg ttccggagct gctcgtagtc ccacacgatc ggcgtcatga
     1621 agaacatcag ggtgacgatg ctggcagtca ccggcgggac atcccggtat ctggtggcga
     1681 tgatcccgat caggatcgcc acccagaccc cgttgagcac gatcagtgcc agcgccggga
     1741 ctgccagcag gctgctccac ccgaaggctc gcggcgcctg cggggggaag atcagcatca
     1801 gggccaccca gaccagcacg ttgtgcccga agaacagcag ttgccgccac accagccgga
     1861 agatgtgcac gctcaacggc gctggtaaat gcttgatcag gccttcgtta cggatgaaga
     1921 cctcggcacc gtcattgatg caggctgcca ggaactgcca gatgatcagg ccgacagcca
     1981 ggtagggcag gaagtcgtgc aatggctggc cgaacagtgc ggcgtagagg atgcccatcc
     2041 cgatcgcctg gacggccagg ctgatggtga tccacagcgg gccgatcacc gatcgccggt
     2101 agccctgctt gatgtcttgc caacccaggt gtccccacag ctgacgttgc cgccaccctg
     2161 cggccagatc accgactgcc cgcggccagc tgcgagacgg cggcgcctgg gcctccgaaa
     2221 tctcggctgc cgtcgcagtg tgctgcacga tccgcgaggg tactcgggca gtggccgaac
     2281 tctggggttg cgggtgctgg tgtcgtcagg ttggcggaac
//
LOCUS       DATADN010000097         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_614298, whole genome shotgun sequence.
ACCESSION   DATADN010000097 DATADN010000000
VERSION     DATADN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614298"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VHH53_01255"
     CDS             <1..1402
                     /locus_tag="VHH53_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2288840.1"
                     /translation="LFLDAPPDASLAATNRSSLEVVKAIRSFPETEFMWSLTANWGGF
                     GGLVAKDWRERERSTEEMYGHVFGAVSQIPGLRVFPRLDPPLPTPGQYDVELVLQSDL
                     PPEQMLETVGQVVCAGWASGKFLYVDTDLKIDLPEARVVIDREQVADLGLDLASVGQE
                     LGTLLGGGYVNRFNYFDRSYKVIPQIGEKDRATVGPLLDLKIKTPSGELVPVSTFTRI
                     ESHTAPRTLNRFQQVNAVRVFGGVQPGVTKEEGLRVLEQAALAAGGQGVVLDYAGESR
                     QIRHEGGALIVTLGFAVILIYLVLAAQFRSFRDPLIVLLGSVPLAISGALIYTFLDLT
                     TINIYSQVGLITLVGLIAKNGILIVQFANTLQERGYEKMAALREASVTRLRPVLMTSA
                     ATVFGHFPLVLVTGPGSEARNSIGTVLVTGMLIGTLFTLFVVPVFYSLIAAQHKPVMM
                     TGEFPVQTAPLAPAAS"
     gene            1748..>2437
                     /locus_tag="VHH53_01260"
     CDS             1748..>2437
                     /locus_tag="VHH53_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX2288841.1"
                     /translation="MPPRSLATATISFGLVSVPVNLFSSSESSAAVSFNMLHAKCGSR
                     LKQQYICASEGTVVEKDEVTKGYEFSKGQYVRFTAEEIKALDEKATNAIDIAEFVPLA
                     AVDRIYLEKVYYLGAGKGGERAYRLLAAALADTGRAALGQYSARGKQYLVLLRPMGDI
                     LVMEQLHYHGELKSADEVPRPEVTIKDAELALARQLIEQASVEEFRPENYRDTVRDRV
                     LEAIQQKVDGQE"
BASE COUNT          401 a          810 c          833 g          393 t
ORIGIN      
        1 cctcttcctc gatgctcccc cggatgcctc gttggcggcg acgaaccgct cgtcgctcga
       61 ggtggtcaag gcgatccgct ccttcccaga gaccgagttc atgtggtcgc tcacggcgaa
      121 ctggggcggc ttcggcgggc tcgtggcgaa ggactggcgg gagcgcgagc gctccaccga
      181 ggagatgtac gggcacgtgt tcggcgcggt gtcccagatc cccgggctcc gcgtgttccc
      241 ccggctcgac ccgccgctcc cgacgcccgg gcagtacgac gtcgagctgg tgctccagag
      301 cgacctgccg cccgagcaga tgctggagac ggtgggccag gtggtctgcg ccggctgggc
      361 cagcggcaag ttcctgtacg tggacaccga cctgaagatc gacctgcccg aggcccgggt
      421 ggtcatcgat cgcgagcagg tggccgacct ggggctggac ctcgccagcg tgggccagga
      481 gctgggcacg ctcctcggcg gcggctacgt gaaccggttc aactacttcg accggagcta
      541 caaggtcatc ccgcagatcg gcgagaagga ccgggccacc gtcgggccgc tgctcgacct
      601 caagatcaag accccgtccg gcgagctggt gccggtgtcc acctttaccc ggatcgagtc
      661 ccacacggcg ccgcgcacgc tgaaccggtt ccagcaggtg aacgcggtgc gggtgttcgg
      721 cggggtgcag cccggggtca cgaaggagga gggcctccgg gtgctggagc aggccgcgct
      781 cgcggccggc gggcagggcg tggtgctgga ctatgccggt gagtcgcgcc agatccgcca
      841 cgagggcggc gcgctgatcg tcaccctcgg cttcgcggtc atcctgatct acctggtgct
      901 cgcggcgcag ttccggagct tccgcgaccc gctgatcgtg ctgctggggt cggtgccgct
      961 ggccatctcc ggcgcgctca tctacacgtt cctcgatctg acgacgatca acatctactc
     1021 ccaggtcggc ctgatcacgc tggtgggcct gatcgcgaag aacggcatcc tgatcgtgca
     1081 gttcgccaac acgctccagg agcgcggcta cgagaagatg gccgcgctgc gcgaggcctc
     1141 ggtcacccgg ctccggccgg tgctgatgac ctccgcggcc acggtgttcg gccacttccc
     1201 gctggtgctc gtcaccggcc cgggctccga ggcgcggaac agcatcggga cggtgctcgt
     1261 caccggaatg ctgatcggca ctctcttcac gctgttcgtg gtgccggtgt tctactcgct
     1321 catcgcggcg cagcacaagc cggtgatgat gactggagag ttcccggtgc agacggcgcc
     1381 gctcgcgccg gccgcgtcgt gagcaggccc gctctcgaca gcaggtagag ctcgagaggg
     1441 gacaccacag aggcacgaag ggcacgaagg acacggagaa aactgcaggt gtgctgttca
     1501 ccaaaacagt tctccgtgtc cttcgtgccc ttcgtgcctc cgtggtaaac ctcaccggcg
     1561 tcgcgacgcg agacgcagtt acccggaatc cgcaagccgg agtgcagaaa gcagcactgg
     1621 agggatagca gggagcactt gcagtccgcg cctcaagcgg gtaactcgct ctcccacaag
     1681 gagctatgta caggcagggc tccgccccct agcggggtcc gattcctgcc tgtatatcag
     1741 tcccccaatg cctcccagat ctctcgctac cgcgaccatt tcgttcggac tggtctcagt
     1801 cccggtcaat ctcttttcct ccagcgagtc ctccgcggcc gtgagcttca acatgctgca
     1861 cgcgaagtgc ggctcccggc tgaagcagca gtacatctgc gccagcgagg gcacggtggt
     1921 ggagaaggac gaggtgacca agggctacga gttctccaag ggacagtacg tgcgcttcac
     1981 ggccgaggag atcaaggcgc tcgacgagaa ggcgaccaac gccatcgaca tcgccgagtt
     2041 cgtgccgctg gccgcggtgg accgtattta cctggagaag gtctactacc tcggcgccgg
     2101 caagggcggc gagcgggcct accgcctgct cgcggccgcg ctcgccgaca ccggccgcgc
     2161 cgcgctgggc cagtactccg cgcggggcaa gcagtacctg gtgctgctcc gccccatggg
     2221 cgacatcctg gtgatggagc agctccacta ccacggcgag ctcaagagcg ccgacgaggt
     2281 accgcggccg gaggtcacga tcaaggacgc ggagctggcg ctggcgcggc agctgatcga
     2341 gcaggcctcg gtggaggagt tccgcccgga gaactatcgc gacacggtgc gcgatcgcgt
     2401 gctcgaggcc attcagcaga aggtggacgg ccaggag
//
LOCUS       DATADN010000098         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1066819, whole genome shotgun sequence.
ACCESSION   DATADN010000098 DATADN010000000
VERSION     DATADN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066819"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /gene="secF"
                     /locus_tag="VHH53_01265"
     CDS             <1..1165
                     /gene="secF"
                     /locus_tag="VHH53_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX2288842.1"
                     /translation="TLGHQAALPAIADTAVTTLLAGGLLFFLASGPVRGFGVTLTIGV
                     LVSLVSALLISRVLADLALCLRPVDRRPQISGISNLGRVRQWLTDRDPALMARHRRWL
                     AVSGVVVALALAGIAIRGLELGVEFTGGRLAQYTGTLPDAETARAVVSDAGFPRAVVQ
                     ESGDGDITVRASGLTDADVAAIGDALGGQMLRDELIGPSLSDELRRGALIAFGVALAA
                     QLAYLAARFRWTFGAGAVAALFANVAVVTGVFAWLGKPIDGVFLAAVLTIIGYTVNDS
                     VVVFDRVREYWSAQRKTPFRRVVSTAVLATLPRTVNTGLSTLVVLAALLLLGGDSLAD
                     FALALLLGIVVGTCSTVSVAAPLAVELESHRSAPPPAPKRKLAPSRRSDGAVV"
     gene            1540..>1834
                     /locus_tag="VHH53_01270"
     CDS             1540..>1834
                     /locus_tag="VHH53_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2288843.1"
                     /translation="MEQTDDPVELVERVAALDLGKAALVACVRVPHDDRPGRRRQEVR
                     EYVTLSRSLLELADWLRCERVELVAMEASSDYWKPVFYLLEAEGFKCWLLNAKH"
BASE COUNT          230 a          580 c          675 g          349 t
ORIGIN      
        1 gaccctcggg caccaggcgg cgctgccggc gatcgccgac accgccgtca ccaccctgct
       61 cgccggtggg ctgctgttct tcctggcgtc cggaccggtc cgtgggttcg gggtgacgct
      121 gacaatcggt gtgctggtct cgctggtctc cgcattgctc atctcccggg tgctcgccga
      181 tctcgcgctg tgcttgcgcc cggtggaccg ccgcccgcag atctccggga tctccaatct
      241 cggcagggtg cggcagtggc tcaccgaccg agaccccgca ctgatggcca ggcaccggcg
      301 ctggctcgcg gtatccgggg tagtagtggc actggcactg gccggcatcg cgatccgcgg
      361 gctcgagctc ggagtggagt tcaccggtgg ccggctggcg cagtacaccg gcacgcttcc
      421 tgatgcggag accgcgcgcg ccgtcgtcag cgatgccggg ttcccacggg cagtggtgca
      481 agagtccggc gacggtgaca tcacggtgcg cgccagtggg ctgactgacg ccgacgtggc
      541 ggcaatcggt gacgcgctcg gcgggcagat gctgcgcgat gaactgattg gtccgagcct
      601 cagcgacgag ctgcgccgcg gcgcgctgat cgcgttcggg gtggcgctgg ccgcgcagct
      661 ggcctacctc gccgcgcgct tccgctggac gttcggagcc ggcgccgtcg cggcactgtt
      721 cgccaacgtc gcggtggtga ccggcgtgtt cgcctggctc ggcaagccca tcgacggggt
      781 gttcctcgcc gcggttctca ccatcatcgg gtacacggtg aacgactcag tggtcgtctt
      841 cgaccgggtg cgggagtact ggtcggcgca gcgcaagacc ccgttccggc gggtggtgag
      901 caccgccgtg ctcgccacct tgccgcgaac ggtgaacacc ggcctgtcca cgctggtggt
      961 cctggcggcg ctactgctgc tcggcggtga ctcgctggcc gacttcgcgc tggcactgct
     1021 gctgggcatc gtggtcggta cctgctcgac ggtgagcgtc gccgccccac ttgcggtgga
     1081 gctggaatcc caccgcagcg caccaccgcc cgcgcccaag cgcaagctcg ctccgagccg
     1141 gcgcagtgac ggcgccgtgg tctgagtccg atccggcggg ggtatccacc gcagaaacca
     1201 ggaggtgtcg cttgctcgcg atcgtcgctg ctcacctggt gatggcgggc ttactgccgt
     1261 tgctgtcggc gcgcagccat cgcacagcgt tcctaatagt cgtcagtgtc ccttgtggga
     1321 cgcccgtgcc gggctgaaaa tcgggtctgt ggctgcctgt gacgtcgtgc gtgcttggtg
     1381 cttgtggtgt gaggccggct aatagtctga cgctgggggc tgttgtgagg ggcgctgcat
     1441 tgttgctttg agcacgcgag tccgcctctg gttatccccg ttggctcccc gggcggccac
     1501 gcctggttca tccgtggcgc gcgtggtggg agtaaggaca tggaacagac cgatgacccg
     1561 gtcgagctgg tcgagcgggt agctgcgctc gatttgggca aggccgcctt ggtggcctgt
     1621 gtgcgggtgc cgcatgacga ccggccgggc cggcggcgcc aagaggtccg cgagtatgtg
     1681 acgttgtcgc ggtcgttgct ggagttggcg gattggctgc ggtgtgagcg ggtggagctg
     1741 gtcgcgatgg aggccagcag cgattactgg aagccggtgt tctatctgct ggaggctgag
     1801 ggatttaaat gctggctgct caacgccaag catg
//
LOCUS       DATADN010000099         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_464156, whole genome shotgun sequence.
ACCESSION   DATADN010000099 DATADN010000000
VERSION     DATADN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464156"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VHH53_01275"
     CDS             complement(<1..482)
                     /locus_tag="VHH53_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2288844.1"
                     /translation="MFSKVLVANRGEIAIRAFRAAYELGCQTVAVFPHEDRNSEHRLK
                     ANEAYEIGEKGHPVRAYLSVEEIVRAAQRAGADAVYPGYGFLSENPKLATACQSAGIT
                     FVGPPPAVLALAGNKSRAVAAAREAGVPVLESCAPSADIDQLMAAADEIGFPMFAKAV
                     A"
     gene            659..1426
                     /locus_tag="VHH53_01280"
     CDS             659..1426
                     /locus_tag="VHH53_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288845.1"
                     /translation="MGRPSYHHYYRWTPARRPFGSLLVMLCVAALASAGMIALAVVAM
                     LLTVGPPILAAVGAVTVARHVHRRRARSRQLPEVAPPASAVAAPPVPDWRATRARFAQ
                     LRSEYGQLECDPLAVLRLPALTDVAVPSTGRFVDAFAEAQALDTDTEPPAAHRARFAT
                     AVERAWRAWHAARDAAERIRLAGIPAPERATVQRAIKLLTMAQDSDHDAERIAAYAKA
                     RAELAKLDRSGTLRLPPAAAAALDVAARGQLPPAAAG"
BASE COUNT          262 a          612 c          576 g          263 t
ORIGIN      
        1 gcgaccgcct tggcgaacat cgggaacccg atctcgtcgg ccgcggccat cagttggtcg
       61 atatcggcgg acggagcgca ggattccagc accggcactc cggcttcgcg cgccgccgcc
      121 accgcccgcg acttgttgcc agccagggcc agcaccgcag gtggcggccc gacgaaagtg
      181 atgccggccg actggcaggc cgtggccagc ttcgggttct ccgacaggaa gccgtaaccg
      241 ggataaacgg cgtcggcccc ggcgcgctgc gccgcccgga cgatctcttc cactgaaaga
      301 tacgcccgaa ccggatggcc tttctcgccg atctcgtagg cctcgttggc cttgagccgg
      361 tgttcggagt tgcgatcctc gtgcgggaac accgccaccg tctggcaacc cagttcgtag
      421 gcggcacgga acgcccggat cgcgatctcc ccgcggttgg cgaccagcac cttgctgaac
      481 acgtgccagc tcccttccac accactggtc gaccggaatc ggtaaccgga ttcatcacct
      541 gcagcatccc aggtgacgag ggtcgctggg gatgcccgaa tccgtcaggc gcggcgtccc
      601 ccccgtgcgg cggccagaag gggtatcaag gaggtggcaa atcggaaagg atccggccat
      661 gggcagaccg tcgtaccacc attactatcg ctggactccg gcacggcggc ccttcggttc
      721 gctgctggta atgctgtgcg tagcggccct ggccagcgca ggcatgatcg ccttggctgt
      781 ggtcgccatg ctgctcaccg tggggccgcc gatcctggcg gcagtgggcg cggtcaccgt
      841 cgcgcggcac gtgcaccgcc gccgggcacg ctcgcggcag ctgccggaag tggcgcctcc
      901 cgcctcagcg gtggctgccc cgccggtccc ggattggcgg gctacccggg cccggttcgc
      961 ccagttgcgc agtgagtacg gccagctcga atgcgacccg ctcgcagtgc tgcggttgcc
     1021 tgcgctgacc gacgtcgcgg tgccatccac cggccgattc gtggacgcct tcgctgaggc
     1081 tcaggcattg gacaccgaca ccgagccacc tgctgcgcat cgcgctcgct tcgccaccgc
     1141 ggtggaacga gcgtggcgcg cctggcacgc cgcacgcgac gcggcggaac gtatccggtt
     1201 ggccggcatc cctgcgccgg agcgcgccac ggtgcagcgg gcgatcaagc tgctcaccat
     1261 ggcccaggat tccgaccacg acgccgagcg catcgcggct tacgcgaaag cacgagcgga
     1321 gctggcgaaa ctggaccgca gcgggacatt gcggttgcca cccgccgcgg ccgccgcgct
     1381 ggacgtcgcc gcccgcggcc agctgcctcc cgccgcggcg ggctaggacc ggacctgcga
     1441 gcagcgcctg gctgccccca gcgcaagtgg gaaacgtgga tttgcaacgt ggtcgcggcg
     1501 gtggccggtg gttgtgccct ccagagcacc tcgtcagccg gtcggtggag accagctcgt
     1561 tggcatgcac aacgagcatg gccaactgca ggggcgctcc ttggcgctgg acacgtcgat
     1621 ccggcgatgc tgatccagtg ccctgagccg accgagaatc gcgaacccca ccaatgcctc
     1681 cagacctgtt cccgtggcac ttcagtcgct ctt
//
LOCUS       DATADN010000100         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_11892, whole genome shotgun sequence.
ACCESSION   DATADN010000100 DATADN010000000
VERSION     DATADN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11892"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VHH53_01285"
     CDS             complement(<1..832)
                     /locus_tag="VHH53_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX2288846.1"
                     /translation="MSAILVASGIALLASILFTPYLIRLFTRQGFGQEIRQEGPQTHQ
                     SKRGTPTMGGVAILLAMWTGYLVAHFTVTGHGITASGLLVLLLTTVLGVVGFLDDLIK
                     IRRQRNLGLNKTAKLVGQLVAAVLFGILAMGFRDADGVSPASTQLSFVRDIAVVSFGA
                     VGFVVFSYLLVSAWSNAVNLTDGLDGLAAGSAAMVLVTYVIVSFWQFTQDCTVQAVPG
                     CYTVRDPLDVAVVAAAAVGGCIGFLWWNAPPAKIFMGDTGSLAIGGLLAGLSVVTRTE
                     L"
     gene            complement(829..2292)
                     /gene="murF"
                     /locus_tag="VHH53_01290"
     CDS             complement(829..2292)
                     /gene="murF"
                     /locus_tag="VHH53_01290"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEX2288847.1"
                     /translation="MIPLTLAEVASAVGGVVHGCDGTEVVSGSVEFDSRRVGPGGLFV
                     ALPGEQVDGHHFAAAAVATGAAGVLAGRPVGAPAVLAPPAHDARSAPYALAADIDGSG
                     AAVLAALAHLARAVVDTLSQTGLEVVGLTGSSGKTSTKDMVAALLAPLGPTVAPPGSF
                     NNEIGHPWTALRADANTRHLVLELSARGPGQIAALCQVAPPSIGVVLNVGSAHLGEFG
                     SRDAVAAAKGELVEALPVAGIAVLNADDVAVAAMDRRTAARVVRFGQSAAAQVRATDV
                     VLNDRGCACFHLVTPAGETPVQLAVPGEHQVSNALAAVAVALELGATPASAAEVLAGY
                     RPASRWRLEVIDRPDGVTVVNDAYNANPESVAAALRTLVAMAGGQRRSWAVLGPLAEL
                     GEAETQAYREIGTLAVQLDVDRLVVVGEQARPMYQAAAGSGPVLVPDVPAALEFLRGE
                     LAAGDVVLIKASRAVGLERVAEGLLGPLTCSTGGSSR"
     gene            complement(2289..3818)
                     /locus_tag="VHH53_01295"
     CDS             complement(2289..3818)
                     /locus_tag="VHH53_01295"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX2288848.1"
                     /translation="MTSSLTETPLRPSRTAPIPVTALATLAGATVLGSPGQNLVTGIT
                     LRAQLVVPGDLFAALPSLGSGRPHGADFAGIALTAGAVAVLTDPAGAHRVALAGATVP
                     VLVHPDPRSVLGALSAHIFGNPSTQLAVLGITGTSGKTTTAFFLEAGLRAAGLATGLL
                     GTVALRIGEQHLLTRGGTTPEAPDLQALLALMVERGVTHLPMEVSSHALALGRVAGTT
                     FAVGAFTNLSQDHLDFHADLEDYFAAKSTLFDGRAAAEVVCIDDAWGQRLVTPKTATV
                     SLAGQATWHAADIVAHRSGSQSFRVLGPGGFDQRATVRLPGPFNVTNALVALAVAHAS
                     GADPQLVLRGVATAEVPGRMQRVDAGQSFLAVVDYAHKPAAVAALLDAMRAQVPGRLL
                     VVLGCGGDRDTGKRLLMGAEAARHADLLVITDDNPRSEDPAVIRAAMLAGARTGPAEV
                     VEIADRAEAIAHAVTRAGPGDAVIVAGKGHETGQQVRGVTYPFSDTEVLAAVLADRSA
                     R"
BASE COUNT          636 a         1389 c         1308 g          485 t
ORIGIN      
        1 gcagttcagt tcgggtcacc acggacaggc cggccagcag ccccccgatg gccagcgagc
       61 cggtatcacc catgaagatt ttcgccgggg gggcattcca ccacagaaac ccgatgcacc
      121 cacccaccgc ggccgcggct accaccgcca catcaagcgg gtcgcggacc gtgtagcagc
      181 cgggaacagc ctggacggtg cagtcctggg tgaactgcca gaacgagacg atgacatagg
      241 tcaccagcac catcgcagcc gaccccgccg ccaacccgtc caggccatcg gtgaggttca
      301 ccgcgttcga ccaggcggac accagcaggt agctgaacac cacgaagccg actgccccga
      361 aggagaccac ggcgatatcc cgcacgaacg acagctgtgt cgatgccggg gaaacaccat
      421 cggcgtcccg gaaacccatc gccaggatcc cgaagagcac cgcggcgacc agctgcccga
      481 ccagcttggc ggtcttgttc agccccaagt tccgctggcg gcggatcttg atcaggtcgt
      541 cgaggaagcc gaccaccccg agcaccgtgg tgagcagcag caccagcagg ccagaagcgg
      601 tgatgccgtg cccggtgacg gtgaagtgcg caaccaggta tccggtccac atcgccagca
      661 ggatggcgac tccgcccatt gtgggcgtgc ctcgcttgct ctgatgagtc tgcggtccct
      721 cctggcggat ctcctggcca aagccttgcc gggtgaacag ccggatcagg tagggcgtga
      781 acaggatgga cgccagcaga gcgatcccgg aggccaccag gatggcgctc acctgctgct
      841 ccccccggtt gagcaggtca ggggaccgag caggccttcg gcaacccgtt ccagcccgac
      901 ggcccgagaa gccttgatca gcaccacatc cccggcagca agctcgccgc gaaggaactc
      961 cagcgccgcg ggcacgtccg gcaccagcac cggacccgac ccggcagctg cctggtacat
     1021 cggtcgcgcc tgctccccca ccacgacgag ccggtccaca tcgagctgaa ccgcgagggt
     1081 gccgatctcc cggtacgcct gcgtctcggc ctcgccgagc tcggccagcg ggccgagcac
     1141 ggcccagctg cgccgttgcc cgccggccat tgcgaccagg gtccgcaacg cggcggcgac
     1201 cgactcgggg ttggcgttgt aggcgtcgtt gaccacggtc accccgtcgg ggcgatcgat
     1261 gacctccagc cgccagcgtg acgccggccg gtaaccggcg agcacctcgg ctgccgatgc
     1321 tggggtcgca ccgagttcca gggccaccgc caccgcggcc agtgcgttgg acacctggtg
     1381 ctcgccgggc accgccagct gcaccggtgt ctcaccggcc ggggtcacca ggtgaaaaca
     1441 agcgcaaccg cgatcgttga ggacgacgtc ggtagcgcgt acctgggccg ctgcagactg
     1501 cccgaagcgc accacccggg cggcggtgcg tcgatccatc gcggcaaccg cgacgtcgtc
     1561 ggcgttgagc accgcgatcc cagccactgg cagcgcctcg accagctcgc ccttggcggc
     1621 ggccaccgcg tcccgggagc cgaactcgcc gaggtgcgct gaaccgacgt tgagcaccac
     1681 cccgatcgac ggcggtgcca cctggcacag cgcagcgatc tgacccggtc cgcgcgcgga
     1741 cagctccaac accagatgcc gggtattcgc gtcagcgcgc agcgcggtcc aggggtgacc
     1801 gatctcgttg ttgaacgatc ccggtggtgc gacggtgggc cccaaggggg ccagcagcgc
     1861 ggccaccatg tccttggtgg aggtcttgcc cgacgacccg gtcaggccca caacctccag
     1921 gccggtctga ctcaacgtgt ccaccacggc gcgtgccagg tgggccagcg cagccagcac
     1981 cgccgcgccg gaaccgtcga tgtccgcggc cagcgcgtag ggcgccgacc gcgcatcgtg
     2041 ggccggcggg gccagcaccg cgggtgcacc gacgggccgc ccggcgagta ccccggcagc
     2101 gcccgtggcc accgcggcgg cggcgaagtg gtgcccgtcg acttgctcgc cgggcaacgc
     2161 gacgaacagc ccccccggac cgacccggcg ggagtcgaac tcgacgctgc ccgataccac
     2221 ctcggtaccg tcgcagccgt gtacgacgcc gccgactgcg ctggccacct cggccagagt
     2281 cagtgggatc accgcgcact ccggtctgcc aggacggcag cgagcacctc ggtatcggag
     2341 aacgggtacg taacgccccg cacttgctgg ccggtctcgt ggcccttgcc tgcgacgatc
     2401 accgcgtcgc cagggccggc ccgggtgacg gcgtgcgcga tcgcctcggc tcggtcggcg
     2461 atctccacca cctctgcggg accggtgcgg gcgccggcaa gcatcgcggc gcggatcacg
     2521 gcggggtcct cggagcgcgg gttgtcgtcg gtgatcacga gcaggtccgc gtgacgggcc
     2581 gcctcggcac ccatcagcag ccgcttgccg gtgtcccggt ccccgccaca ccccagcacc
     2641 accagcagcc gacctgggac ctgggcgcgc atcgcgtcga gcaaggccgc gacggccgcc
     2701 ggtttgtgcg cgtagtccac taccgcgagg aaactctgac cggcgtctac ccgctgcatc
     2761 cggccgggca cctcggcagt ggcgaccccc cgcagaacca gctggggatc ggcgcccgag
     2821 gcgtgcgcga cagccagcgc aaccaacgcg ttggtgacgt tgaacggacc cggcaaccgc
     2881 accgttgcac gctggtcgaa cccgccggga ccgaggacgc gaaacgactg gctgcccgac
     2941 cggtgggcca cgatgtcggc cgcgtgccag gtcgcctggc cggctaatga cactgtcgcc
     3001 gtcttaggcg tgaccagccg ctgtccccag gcatcgtcga tgcagaccac ttcggcggcg
     3061 gcccgcccgt cgaacagtgt cgacttggcg gcgaaatagt cctcgagatc ggcatggaag
     3121 tccagatggt cctgggacag gttggtgaac gcgccgaccg cgaaggtggt acccgcgacc
     3181 cggcccagcg ccagcgcgtg gctggacacc tccatgggca ggtgagtgac gcctcgctcg
     3241 accatcagag ccagtagcgc ctgcaggtcc ggtgcctccg gagtggtgcc tcccctggtg
     3301 agcaggtgct gctcaccgat ccgcagcgcc acggtgccga gcaacccggt ggccaggcca
     3361 gccgcccgca accccgcctc gaggaagaac gccgtggtgg tcttgccaga ggtaccggtg
     3421 attcccagca ctgccagctg ggtggacggg ttgccgaaga tgtgggcgga cagcgcgccc
     3481 agcaccgacc gtgggtcagg gtgcaccagc accggcacgg tcgcaccggc cagcgccacg
     3541 cggtgtgcac cggcggggtc agtgagcacg gcgacggcac cggcggtcag cgcgatgccc
     3601 gcgaagtcgg cgccgtgcgg tcgcccggaa cccaggctgg gcaacgccgc gaagaggtcg
     3661 ccgggcacga ccagctgggc ccgcagggtg atacccgtga ccaggttctg acctggggat
     3721 cccaggacgg tcgcgccggc cagggtggcg agcgcggtca ccgggatcgg ggcggtgcgc
     3781 gacggccgca gcggtgtctc ggtcaacgag gaagtcac
//
LOCUS       DATADN010000101         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1067258, whole genome shotgun sequence.
ACCESSION   DATADN010000101 DATADN010000000
VERSION     DATADN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067258"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            647..>1523
                     /locus_tag="VHH53_01300"
     CDS             647..>1523
                     /locus_tag="VHH53_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2288849.1"
                     /translation="MMLRSPRRAVPLLAAAFTFAACGADSLGGVTPPDDPSLVVIPGK
                     GSATTLDVAGWNIEWFGSTGSGPTNETLQLSNARDVISGTDFDIWGVAEITGQAAWNS
                     LESSLTGYTGFLAGESIVTSGSTYYASTEQKVGILYKSSLATLVGAKVILTAHDTDFA
                     GRPPLEVRLDVTLNGTTEEVVVIVLHMKAFDDVPSWQRRQNASNALKSYLDTTYPTQK
                     VIVVGDWNDDVDTSITSGQASPYQNFVSDAADYAFPTRALSLAGTSSTTSYPDMIDHH
                     LVTSELNATLVPGSTE"
BASE COUNT          259 a          509 c          499 g          256 t
ORIGIN      
        1 ccggttttct gccccaccgg gagccgcact ggtactcgtg acttcttaga ccgcactcac
       61 ggctgtcatt ccgagggagg cgcccgcgcg atcctggcgt gtgctggatg tgtgcgcgcc
      121 gaccgaggaa tctactctcc cgaacgggtg gccggctcac ctgctcgatc cggcccgttg
      181 gaatcccgtt gcgtgagcga cgcccacagt gcggtacagg tgtgctgggc gggttcctgg
      241 actgaggaca agccacgagc gtgggcgagt agattcctcg gtcggcgcca gagatcgcgc
      301 cccggcaaag atcccgtggg cgcctccctc ggaatgacag atcgcggcgt gtgggcgagg
      361 gtgtctcacg gtgcattgca actaaatccg tgatgagcca aaaagagagg acgcagaggg
      421 ccgcatcggt tctccgcgtc ttcgcttttc gctccgcgcc ctccgcgtga aacgttccct
      481 cgattgccat gtaactcgga acgacagcgg gactggactt tccgtctggt ggatcggcca
      541 cctgtctcgt acgttacccg ccatcgtacg aggtggtagc cgttctccat tccgcgcctc
      601 atcccgaggc gcggtgccgc gtcttcccca tccacaggga gcctggatga tgcttcgcag
      661 cccgcgccgg gccgtgccgt tgctggccgc cgccttcacc ttcgccgcct gcggggcgga
      721 ttcgctgggc ggcgtcaccc cgccggatga cccgtccctc gtggtgatcc cgggcaaggg
      781 atcggccacc acgctggacg tggccggctg gaacatcgag tggttcggca gcacggggag
      841 cggaccgaca aacgagacgc tgcagctgag caacgccagg gacgtcatca gcggcaccga
      901 cttcgacatc tggggcgtgg cggagatcac gggccaggcg gcgtggaaca gcctggaaag
      961 cagcctcacc ggctacaccg gctttttggc tggcgagtcc atcgtcacca gcggctccac
     1021 ctattacgcg tccaccgagc agaaggtggg aatcctgtac aagagctcgc tggccacgct
     1081 ggtgggggcg aaggtgatcc tcaccgccca tgacaccgac ttcgccgggc ggccgccgct
     1141 ggaggtgcgg ctcgacgtga cgctgaacgg gaccaccgag gaggtggtgg tcatcgtgct
     1201 gcacatgaag gcgttcgacg acgtgcccag ctggcagcgg cggcagaacg cctccaacgc
     1261 gctcaagagc tacctggaca ccacctatcc cacgcagaag gtgatcgtgg tgggtgactg
     1321 gaacgacgac gtggacacca gcatcacgtc cggccaggcg tcgccgtacc agaacttcgt
     1381 gagcgacgcg gcggactacg cctttcccac ccgcgccctg tcgctggccg gcacctcgtc
     1441 cacgacgagc tacccggaca tgatcgacca tcacctcgtc accagcgagc tcaacgccac
     1501 gctggtgccg gggtccacgg agg
//
LOCUS       DATADN010000102         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1368593, whole genome shotgun sequence.
ACCESSION   DATADN010000102 DATADN010000000
VERSION     DATADN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368593"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..620
                     /locus_tag="VHH53_01305"
     CDS             360..620
                     /locus_tag="VHH53_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288850.1"
                     /translation="MKNDASCSRRPDTATRNMARAIPDSVERLVAGANTRELMECMGH
                     NSPAVALRYQHVMAGRAGPSPPLWTSSSKPPPASFRSARPRA"
     gene            complement(709..781)
                     /locus_tag="VHH53_01310"
     tRNA            complement(709..781)
                     /locus_tag="VHH53_01310"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(746..748),aa:Ala,seq:tgc)
     gene            1165..1578
                     /locus_tag="VHH53_01315"
     CDS             1165..1578
                     /locus_tag="VHH53_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2288851.1"
                     /translation="MTHHEHPNVAVVREGFEAVQRGDTAWMDQHLADDVIWHVGGNSK
                     WAGAYQGKQQVLEYFARQAQATSGPPSVDIHDIVGNDEHVVVLGSAKATARDGSSAEW
                     KYTQIFHIRDGKATEVWGMAENDAAVDPFLDGLPD"
     gene            complement(1706..2644)
                     /locus_tag="VHH53_01320"
     CDS             complement(1706..2644)
                     /locus_tag="VHH53_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEX2288852.1"
                     /translation="MNEQQNHQATPDAGPGYLRIGQLAKRAGVSPELLRAWEQRYGLL
                     QPTRTPGGFRLYSAADEARVRRMQSLVSGGLAAAQAARLVLSGGEPAPRTASPSTTTL
                     DDAAGNLTASLDRLDEQAANTALDRLFAAYTVETVLQEVLIPYLHRLGERWETGEVSV
                     AQEHFASNLIRGRLLGLAQGWGQGQGPGAILACVPGEQHELGLLAFGVALRRRGWRIT
                     YLGTDSPVDAVADIARSIAPAVVVLLGMNPERFLDHAHEIKQLAKQVPVVIAGPGATP
                     EAARQTQTRVLDQDPVSAAETIDRDHSARGGEPRRS"
     gene            2757..3002
                     /locus_tag="VHH53_01325"
     CDS             2757..3002
                     /locus_tag="VHH53_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX2288853.1"
                     /translation="MKRVNALAAILTIVGGLNWGLVGLFKFDLVAAVFGGMRFGEVNV
                     GSRIVYTLVGLSALYLASQLRTLISSSEPTPASSVSS"
     gene            3027..3650
                     /locus_tag="VHH53_01330"
     CDS             3027..3650
                     /locus_tag="VHH53_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX2288854.1"
                     /translation="MLRLLRPRRAAVVGVLGMALLATACSNDTGTSASQAPAAPPTTA
                     SSMTASAPFGAACSAVPASGAGSLEALAQDPVATAASNTPDLSTLVTAVKKADLVNTL
                     NSAQGITVFAPTNDAFGALPKSTLDKALGDPKGLLTTVLTYHVVQGQLTPDQLAGTHK
                     TLQGGTLQVTGSGEDFKVNGNSSVVCGNVKTANATVYLIDQVLLPKS"
BASE COUNT          594 a         1308 c         1250 g          553 t
ORIGIN      
        1 ccgactccac cgctggcagc gcatccgcgg cccagacggc cagtggtacc gcgaatgcca
       61 agcctgcgga acacagttga tcgatcgcca cagggagggt cctccgccta ccgcgatcgg
      121 ctgatccggc gagctacagg agtcgctact ggtcctagct ccgcgtcgtc ctggtggtcg
      181 tcgccgtcgc ccaccgtagg ctcggccccg gtcttcggcc acgacctcga cagcggatcg
      241 ggcgctgccg tcctcggcgg tccagctccg ctgccggagc cgacccacga ccacgacccc
      301 ggctgccctt ggccagggac tcgaccgcat gctcggcctg gtcgcgccac acgatcacgg
      361 tgaagaacga cgcctcctgc tcccgccgcc cggacaccgc cacccggaac atggcccggg
      421 cgatcccgga ctcggtggag cggctggtgg ctggggcgaa cacccgggag ttgatggagt
      481 gcatgggcca caactcgcca gcggtggcgc tgcgctacca gcatgtgatg gctggccggg
      541 ccgggccatc gccgccgctc tggacgagct cgtccaagcc gccgccagcc tccttccgga
      601 gcgcccggcc gagggcctag tggcacgctg ctggcacgaa accggcgcgc gaagccggag
      661 ggtcccggcc gccgctgccc gtggggcgct gacctgggtt gttgtgggtg gagatgatgg
      721 gactcgaacc cacgaccccc tgcttgcaaa gcaggcgctc tgccaactga gctacatccc
      781 catgctgacc tgcggttttg cgtcgcaccg cgcctcgtgc acggtagcat agcgctccac
      841 caggaccata acagcagcga acgcctcaac gcctctatga ggtcgcgatg gtgtcggcac
      901 ggttgaccgc gagcggtgga ggcaacccga gctcccggga tcagcccggg tcgccctcag
      961 cgtacggact ggacatcatg tccagcgccc ggtgatccgg tggtcgcgtt cggctggaca
     1021 ccgaggcggc aacggcggtc tacgctgccg gccgccctgt atccggcgat gcctcgtctg
     1081 cccgccacca ggttctcagc gggcagcaga ccaaggtaga ctgccggcga ccaggccagg
     1141 gggccgcctg cggaaggagt ggacatgacc caccacgagc atcccaacgt cgccgtcgtc
     1201 cgggaaggct tcgaggcggt tcagcggggc gacactgcct ggatggacca gcatctggcc
     1261 gacgatgtca tctggcatgt cggtggcaac agcaagtggg ccggggccta ccagggcaag
     1321 cagcaggtgc tggaatactt cgcccgccaa gcccaggcca ccagcgggcc gccgtcggtg
     1381 gatatccatg acatcgtggg caacgacgag cacgtggtcg tccttgggag cgcgaaagcg
     1441 accgcgcggg acgggtccag cgccgagtgg aagtacaccc agatcttcca catccgggac
     1501 gggaaggcga ccgaggtctg ggggatggcc gagaacgacg ccgcggtcga cccgttcctg
     1561 gatggcctgc ccgactgagc gccggcgaag ggcgactcgg cgatccatcg gggcgttcgt
     1621 cggggtggtg cctggccgag tcgtgaagtg gcagtgctag cgcaccagca ggtccatgcc
     1681 ggagggtttg accatgggcc gggcctcacg accgacgcgg ttcgccaccg cgggccgaat
     1741 ggtcacggtc gatggtctcg gcggcgctca ccgggtcttg atccaggaca cgggtctgcg
     1801 tctgccgggc ggcctcgggg gtggcgcccg gacctgcgat cacgactgga acctgtttgg
     1861 cgagctgctt gatctcgtgc gcgtggtcca ggaacctctc tgggttcatg cccaggagga
     1921 ccacgaccgc gggtgcgatg gagcgggcga tgtcggcgac cgcgtcgacg gggctgtcgg
     1981 tgccgaggta ggtgatccgc cagccgcggc ggcgcaaggc gaccccgaag gccagcaggc
     2041 cgagttcgtg ctgttcgccc ggtacgcagg cgagaatggc gcctgggcct tggccctggc
     2101 cccagccttg ggcaagaccg aggaggcgtc ctcggatcag gttggaggcg aagtgctcct
     2161 gggcgaccga cacctcgccg gtctcccatc gttcccccag cctgtgcagg tacggaatga
     2221 ggacctcctg gaggaccgtc tcgacggtgt aggcggcaaa gagccggtcg agggcggtgt
     2281 tggcagcctg ctcatccagc cggtcgagtg aggcggtgag gttgccggcc gcatcgtcca
     2341 gggtggtggt cgacggcgag gccgtgcgcg gtgctggttc gccgccgctg agaaccaggc
     2401 gggccgcctg ggcggcggcg agtccaccgg agacaaggct ctgcatgcgc cgcacccggg
     2461 cttcgtcagc agcgctgtac agccggaagc cacccggtgt ccgtgtcggc tggaggagcc
     2521 cgtagcgctg ctcccaggcc cggagcagct cgggactgac cccggcccgt ttggccagct
     2581 ggccgatccg aaggtagccc ggtccggcgt cgggcgtggc ctggtggttc tgctgttcgt
     2641 tcacaccctt actgtacaag caatgaacga tctctgtaca gactcttgac aagtgaccga
     2701 agtgctggca atctctccac aggacctaga caaactctag acagaagggg aggtccatga
     2761 agagagtcaa cgccctggcc gcgatcctca cgatcgtcgg cgggctcaac tggggcctgg
     2821 tcgggctgtt caagttcgac ctggtcgccg ccgtgttcgg cggaatgcgc ttcggcgagg
     2881 tgaacgtcgg cagccgcatc gtctacacgc tcgtcgggct gtccgcgctc tacctggcca
     2941 gccagctccg gacgctcatc agcagctccg agccaacccc cgccagctcc gtcagctcct
     3001 gaccgagtcc ccgaaaggat cagacaatgc tccgtcttct ccgtccccgc cgcgccgccg
     3061 tggtcggcgt cctcggcatg gccctcctgg ccaccgcctg cagcaacgac accggcacct
     3121 cggccagcca ggcccccgcc gccccgccca cgaccgcctc gtcgatgacc gcgtcagccc
     3181 cgttcggggc cgcctgctcg gccgtgccgg ccagcggcgc gggcagcctg gaggccctgg
     3241 ctcaggaccc ggtggccacc gccgcctcca acaccccgga cctgtccact ctggtcaccg
     3301 cggtcaagaa ggccgacctg gtcaacaccc tgaacagcgc ccagggcatc accgtgttcg
     3361 cgcccaccaa cgacgccttt ggcgccctcc ccaagtccac cctcgacaag gcccttggcg
     3421 accccaaggg gctgttgacc accgtgctca cctatcacgt ggtccagggc cagctgaccc
     3481 ctgaccagct cgccggcacc cacaagactc tccagggcgg cacgctccag gtcaccggga
     3541 gcggcgagga cttcaaggtc aacggcaact ccagcgtggt ctgcggcaac gtcaagaccg
     3601 ccaacgccac cgtctacctc atcgaccagg tgctcctgcc caagagctga ccactacaca
     3661 gcaccgagct cggcgcggcc accggcacca catgcccggt ggccg
//
LOCUS       DATADN010000103         4599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_917152, whole genome shotgun sequence.
ACCESSION   DATADN010000103 DATADN010000000
VERSION     DATADN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917152"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VHH53_01335"
     CDS             <1..851
                     /locus_tag="VHH53_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX2288855.1"
                     /translation="VLAPLRRGRGDPTMRVDADATVWRTSTTPAGAATLALRRANGTV
                     HATAWGPGATWALDGLPALLGADDDDSAFISHHALVAHARRRMPGLRLGATHRVWDVL
                     VPAVLEQKVTGIEARRSWRELCWRFGQPAPGPGELRVPPSPAAVRAIPDWEWHRAGVD
                     SARRRALLAAAAVAHRLEGAVALGGKAGRDLLCKVPGIGVWTAAEVAQRAWGDADAVS
                     VGDFHIPSVVGYALLGRPLDDEGMLEVLAPYLPQRHRAVRYVEASGFRRPRFGPRFSP
                     RDYRAL"
     gene            complement(836..1486)
                     /locus_tag="VHH53_01340"
     CDS             complement(836..1486)
                     /locus_tag="VHH53_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288856.1"
                     /translation="MSGAAALAARYRPGADADATQPVHLVPLPVRTAVGAVSAFCGTL
                     LSMPTLEAVESGQGVPCPMCVLQHGATTQAPQNETDYSSLDWPVQLQGDQLVLPLGHC
                     ATAQIVPMDLAAAITPILAALDRPVPVLIHPDAPGCGVVIAGEPYGVPLPWPETAQVV
                     TGMLPLPPSRTPHGPVHWYQDAPHHPLTTCREIDVFSAMRAAGPAAQQQSGTHRAR"
     gene            complement(2058..2297)
                     /locus_tag="VHH53_01345"
     CDS             complement(2058..2297)
                     /locus_tag="VHH53_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HEX2288857.1"
                     /translation="MLNSVCNGTSATRLTGVTWRKSKHSGQIGNCVETASLDSGEVAV
                     RNSRYPSGPALIFTRDEMAAFLAGAKDGEFDHLTS"
     gene            complement(2475..3371)
                     /locus_tag="VHH53_01350"
     CDS             complement(2475..3371)
                     /locus_tag="VHH53_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2288858.1"
                     /translation="MATDDSTDASHRSGAAQSSSPTARRIILGARLRRLREAAEISRA
                     EAGYAIRGSESKISRLELGRVSFKPRDVTDLLTMYGVTDVEEREVFLGLVKASNEPGW
                     WHRYTDLVADWFQDYLGLEESASRIQTFEVQFLPGLMQTEDYAKAIATRGWSQLASQR
                     ADRQVAVRIRRQELLARPDAPQLWAIIDESVLHRPIGGRRVMLAQIEHLLELTKRPNI
                     TLQIVPYPLSGYAAEGSFTMLRFAEPELPNVVYIEYLSGALYLDRQSETELYGRAFDR
                     LTVDAKTPDHSRQLLAKVRAEI"
     gene            3558..4397
                     /locus_tag="VHH53_01355"
     CDS             3558..4397
                     /locus_tag="VHH53_01355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2288859.1"
                     /translation="MSGTGDPLSDDVTPVFIDTTKPSIARVYDAVLGGKDNYEIDRQL
                     LRDLLKITPEARLLAWDNRNFLIRVTRFLAEEAGITQFLDCGSGLPTAENVHQVAQRI
                     QPDARVVYIDNDPIVLAHGRALLEENDQTHLVDADIFEPHTIIHNETVRKYLDFSEPI
                     ALFQMGTLLHYDGDRSLQDIMEEFIDALPPGSYVASSHFLDPETPAELSELAKTIEQA
                     FLRNLGNGYFRTRAELESTYPGLELVPPGLVPCTDWWPDGPRIKPMAPAQHLLVGAVG
                     RKP"
     gene            4442..>4599
                     /locus_tag="VHH53_01360"
     CDS             4442..>4599
                     /locus_tag="VHH53_01360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2288860.1"
                     /translation="MPGTDAPLPDDVTPVYIDTTKASIARVYDAFLNGKDNYEVDREV
                     LRRVQQVAP"
BASE COUNT          771 a         1500 c         1499 g          829 t
ORIGIN      
        1 ccgtcctggc cccgctgcgc cgtggccggg gcgatccgac gatgcgggtg gacgccgacg
       61 ccaccgtgtg gcgcacaagc accacgccgg cgggtgctgc caccctggcg ctgcggcgag
      121 ccaacggcac cgtgcacgca accgcgtggg ggccgggcgc gacctgggcg ctggacgggc
      181 tgcccgcgct gctcggtgcc gacgatgacg atagtgcctt catctcgcac cacgcgctgg
      241 tcgcgcacgc tcggcgccgg atgccaggtc tgcgcttggg cgccacccac cgggtgtggg
      301 atgtgctggt gcccgcggtg ctggagcaga aggtcaccgg tattgaggct cggcgctcct
      361 ggcgcgagct gtgctggcgg ttcggacaac cggcgccggg ccctggcgag ctgcgagtgc
      421 cgcccagccc ggcggcggtg cgtgcaatcc ccgactggga gtggcaccgc gcgggggtgg
      481 attccgcgcg gcgccgggcc ctgctggcgg cggctgcggt ggcgcaccgg ctggagggcg
      541 cggtggcact gggcggtaag gccggtcgtg atctgctgtg caaggtccca ggaatcgggg
      601 tctggacggc cgccgaggtg gcccaaaggg cctgggggga cgccgatgcg gtgagtgtcg
      661 gcgacttcca cattccctct gtcgtcggtt acgccctgct cggccggcca ttggacgacg
      721 agggcatgct cgaagttctc gcgccctact tgccgcagcg gcaccgggcg gtgcgctatg
      781 tcgaggcctc cgggttccgc cgcccccgct tcggcccccg attcagtcct cgcgactacc
      841 gcgccctgtg agttccgctt tgctgctgcg cagccggccc agcggcgcgc atcgccgaga
      901 agacgtcgat ctctcggcag gtggtcagcg gatggtgtgg tgcgtcttga taccaatgca
      961 ccggcccgtg aggagtccgg ctggggggta gcggcagcat cccggtgacc acctgcgccg
     1021 tttcgggcca gggcagcggc acgccgtagg gctcacctgc gatgaccacg ccgcaccccg
     1081 gagcgtccgg gtggatcagg accggcaccg ggcgatcaag tgcggccaag atcggtgtga
     1141 ttgccgcggc cagatccatg ggcacaatct gtgcggtggc gcagtgcccc agcggtagca
     1201 ctagctgatc gccctgcagc tgcaccggcc agtccagcga gctgtagtcc gtctcgtttt
     1261 gcggggcctg cgtcgtggcg ccgtgctgta gcacacacat cgggcacggc acgccctgac
     1321 ccgattcgac ggcttccagc gtcggcatgg acagcaacgt cccacagaac gcgctgaccg
     1381 cgccgaccgc ggtccggacc ggtaatggga ccagatgtac gggctgcgtt gcgtctgcat
     1441 ccgccccggg ccggtaacgc gcggccagcg ccgcagcgcc gctcacactt gccccgccac
     1501 tgtcaagatc actgacctgg cacagcgggg tggagtctgg tcattcggtg tggttgtggg
     1561 gcacggcgta gcggggggaa ttcgttatgc tcctcggctc cgccgtggct cgagttgacc
     1621 gtggggccag tgcgagtccc cgcggcaggg gtggctggaa tcttgcccgt gccgagaacg
     1681 accctgccga ttcataggcc tgttgtgcca tcgcggtacc ggcgacgccc gagatagcgg
     1741 acgagtactc cggccttgct aggcaacggt tcacggtcaa gccgctgcac gatcgtttcg
     1801 catgaccgac aacgacgacc tggggggctc atcaaggatg agacacacac cgtagtaccg
     1861 cagagtgccg catatcgtcc tgagccgacc gaaaacccga ttgccatgtt gtcatcgctg
     1921 atcgcatggt cggtacagtc gaaggcgcag gtgaaccacg tcatgaactt tgcacgggtc
     1981 ctcggttcgt gcatcttccc tgcgtcctgg ctccacactg tcggtggact agttgcttga
     2041 cctcggcgct ggtcgactca gctggtgagg tggtcgaact cgccatcctt ggcgccggcc
     2101 aagaaggcgg ccatttcatc acgggtgaag atcagagctg ggccactcgg gtagcgcgag
     2161 ttgcgcactg cgacttctcc gctgtccagc gatgctgtct ccacacagtt gccgatttgg
     2221 ccactgtgct tgctcttccg ccatgtcacc ccggtaagtc gggtggcgga cgtgccattg
     2281 caaactgagt tcaacataga tcgcctcctc tagcggatgc atctgcacgt gcatcacgct
     2341 aaagctagca cgtgctctcg cacgcttcaa atgcacgtgc agatgaggtg gccggcgctc
     2401 ggatgcagca acggacgtgg agaactcggc gcgcgagact gggctcagga gcggctcggc
     2461 cgcagctcag ctgctcagat ctcggcgcgc accttcgcca gtagttgcct ggagtggtcg
     2521 ggagtcttgg cgtccaccgt gagccggtcg aaggccctgc cgtacagctc ggtctcggat
     2581 tgtctgtcca ggtagagcgc gccgctgagg tactcgatgt acaccacgtt cggcagctca
     2641 ggttcagcga agcgcagcat ggtgaacgag ccttcggccg cgtatccgct gagcgggtag
     2701 ggcacgatct gaagggtgat gttgggccgc ttggtgagct ctaggagatg ctctatttga
     2761 gccagcatca cccgcctgcc gccgatcggt cggtgcagta cggactcgtc gatgatcgcc
     2821 catagctggg gtgcatcagg ccgggcgagc agttcctgtc gtcgaatccg caccgcgacc
     2881 tggcggtctg ctctctggct cgccagctga gaccaaccac gcgtcgcgat tgccttcgcg
     2941 taatcctcgg tctgcatcaa gccggggagg aactgcactt cgaaagtctg gatccgggag
     3001 gctgattcct ccagaccaag gtagtcctgg aaccagtcgg ccaccagatc ggtgtagcga
     3061 tgccaccagc cgggctcgtt ggacgcctta accagcccga gaaaaacctc gcgctcctcg
     3121 acatccgtca ccccatacat ggtgagcagg tcggtcacgt cccgcggctt gaaactgacc
     3181 ctaccgagct cgaggcggct gatctttgac tcagaaccac ggatggcgta gccagcctcc
     3241 gcgcgtgaga tttcggcagc ctcgcgcagt cgacgcaggc gtgcgcccaa gatgatgcgg
     3301 cgcgccgtgg gactgctgct ttgagcagcg ccagaccggt gggatgcatc ggtggaatcg
     3361 tctgtggcca tgacgcacct ccggggtgta ttcaagtgtc ccccatcgac ttagtgctgt
     3421 cgctcgggga cactcgatca ctcaacgtac cgcgctgtgg cgcacggtca acacaccacc
     3481 catggtttac cgggcgcgtc catccccata ccctggctgc gttcaacccc gtgctgcaac
     3541 ctcctgggag ggcctcgatg tccggtaccg gcgacccact gtcggatgat gtcacgccag
     3601 ttttcatcga cacgaccaag cccagcattg cccgggtcta cgacgcagtc ctgggcggta
     3661 aggacaatta cgagatcgac cgacagcttc ttcgggacct cctgaagatt acaccggagg
     3721 ccaggttact cgcgtgggac aaccgaaatt tcctgatccg ggtgacccgg tttctcgccg
     3781 aggaggcggg catcacccag ttcctcgact gcggatcggg actgcccacg gcggagaatg
     3841 ttcaccaggt tgcccagcga atccagcctg acgcccgcgt agtctacatc gacaacgacc
     3901 cgatcgtgct cgcacacggc cgggcgctgc tcgaggagaa cgatcaaacc cacttggtcg
     3961 atgctgacat cttcgagcca cacacaatca tccacaacga gaccgtacgc aagtaccttg
     4021 acttctctga gccgatcgcg ctgttccaaa tggggacgtt gctccactac gacggcgacc
     4081 gcagtctgca ggacatcatg gaggagttca tcgacgccct gccgccgggc tcttacgtgg
     4141 cgagtagcca cttcctcgat cctgagaccc ccgccgagct gagcgaactc gctaagacga
     4201 tcgaacaggc cttcttgcgc aacctgggca acggatattt ccgcacccgc gccgagctcg
     4261 agagcacgta tccgggcttg gaactggtcc cgcccggcct ggtgccgtgc accgactggt
     4321 ggcccgatgg cccgaggatc aagccgatgg cccccgcgca gcaccttctc gtcggagcag
     4381 tcggccgtaa accctgacct cgcgttgtcc agacccgtac tgcaatgcct gtgaggactc
     4441 gatgcctggt accgacgccc cactgccgga tgatgtcaca ccagtttata ttgacacgac
     4501 caaggcgagc attgcccggg tctacgacgc gtttctgaac ggtaaggaca attacgaggt
     4561 cgaccgagag gtacttcggc gggtccagca ggtcgcccc
//
LOCUS       DATADN010000104         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_917231, whole genome shotgun sequence.
ACCESSION   DATADN010000104 DATADN010000000
VERSION     DATADN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917231"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /gene="nuoH"
                     /locus_tag="VHH53_01365"
     CDS             complement(<1..1118)
                     /gene="nuoH"
                     /locus_tag="VHH53_01365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX2288861.1"
                     /translation="MTPTQALLADDPLWLTALKVVVIFAFLMVMTLFMIWMERRVVGR
                     MQHRPGPNRVGPFGLLQSLADGLKLAFKEDITPVQADRWVYVLAPVISAVTAFAAFSV
                     IPLGGQVTIFGEETVLQITDLSVGVLVVLAASSLGVYGIVLSGWASGSPYPLLGSLRS
                     AAQVISYEIALGMSIVAVILYSGSLSTADIVEAQSQQWWFVFLLFPSFAVFCVAMVGE
                     VNRAPFDLPEAESELVGGFHTEYSSLKFALFFLAEYVNMVTVSAMATTLFLGGWHAPW
                     PLAGGPLDTGWWGPLWFIGKTLAFLFVFIWLRGTLPRFRYDQFMALGWKVLVPVSLIW
                     IVVVSGVRALRGADVTGPEILVVGGILLGALVLVAFLFV"
     gene            complement(1123..>1634)
                     /locus_tag="VHH53_01370"
     CDS             complement(1123..>1634)
                     /locus_tag="VHH53_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX2288862.1"
                     /translation="RVLDSLAIEMDVDLFTQTPAAAWAGLVALGRYTGARLDPPAVPP
                     APGPEVGEGQALLATWRLLLDNGSMQDGDPHLAGTARPAVAMLSEATAAELAVHIGGK
                     VTVSTRQGSVTLPAAAADLPAGVVWIPGNSGPATVRSSLGAGHGAVVTVRAGNDASTY
                     RHTDRGVGR"
BASE COUNT          327 a          559 c          538 g          210 t
ORIGIN      
        1 acgaacagga aagccacgag gaccagtgcg cccagcagga tgccgccgac cacgaggatt
       61 tcgggtccgg tgacgtcggc gccgcgcagc gcccggaccc cggagaccac cacgatccag
      121 atgaggctga ccggtaccag caccttccag cccagcgcca tgaactggtc ataacgaaac
      181 cggggcaggg tgccccgcag ccagatgaac acgaacagga aagccaaggt cttgccgatg
      241 aaccacagcg gaccccacca gccagtgtcc agcggcccgc cggccagtgg ccacggagcg
      301 tgccagccgc ccaggaacag cgtggttgcc atggccgaaa cggtgaccat gttgacgtac
      361 tcggcgagga agaacagcgc gaacttcagc gacgagtact cggtgtggaa accaccgacc
      421 agctccgact ccgcctcggg caggtcgaag ggggcacggt tgacctcgcc gaccatcgcc
      481 acgcagaaca ccgcgaagct ggggaacagc aggaacacga accaccactg ctgagactgc
      541 gcctccacga tgtccgccgt ggacagcgag cccgagtaca ggatcaccgc gacgatcgac
      601 atcccgaggg cgatctcata ggagatcacc tgggcggccg agcgaagcga gcccagcagc
      661 gggtagggcg agcccgacgc ccagccggac agcacgatcc cgtagacgcc cagcgaggac
      721 gcggccagca ctacgagcac tccgaccgac aggtcggtga tctgcagtac ggtctcctcg
      781 ccgaagatcg ttacttgacc gccgagggga atgacggaga aggcggcgaa tgcggtaacc
      841 gcggagatca ccggagccag aacgtacacc cagcggtcgg cctgaaccgg cgtgatgtcc
      901 tccttgaacg ccagcttgag cccgtcggcc agggattgca gcagcccgaa gggtccaacc
      961 cggttgggac cgggccggtg ctgcatgcgc ccgaccaccc gacgctccat ccagatcatg
     1021 aacagggtca tgaccatgag gaaggcgaag atcacaacca ctttcagcgc ggttagccac
     1081 agcgggtcgt cggcaagcaa cgcctgggtg ggtgtcatgg ctttacctcc ccacgcctct
     1141 gtcggtgtgt cggtaggtgc tcgcgtcgtt gccagcccgc acggtgacca cggcgccatg
     1201 gccggcaccg aggctgctgc ggacggtagc gggtccggag ttacctggaa tccacaccac
     1261 ccccgctggc aggtcagcag ccgcggccgg cagcgtcacc gacccctgtc gggtggacac
     1321 cgtgaccttt cctccgatgt gcaccgcgag ctcggcggcg gtggcctcgg acagcatcgc
     1381 caccgcgggc cgtgcggtgc cggccaggtg tggatcgccg tcctgcatcg atccgttgtc
     1441 cagcagcagg cgccaggtag ccagcagcgc ctgcccctcg ccaacctccg ggcccggcgc
     1501 cggtggcact gctggagggt ccagacgggc accggtatac ctgccgagtg cgacgaggcc
     1561 cgcccatgcc gcagctggag tctgggtgaa caggtcgacg tccatctcga tggcgaggct
     1621 gtccagcacg cggc
//
LOCUS       DATADN010000105         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_163246, whole genome shotgun sequence.
ACCESSION   DATADN010000105 DATADN010000000
VERSION     DATADN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163246"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VHH53_01375"
     CDS             <1..1255
                     /locus_tag="VHH53_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HEX2288863.1"
                     /translation="EILAALAEAQAEVGLVPADAARAIRDCADVTLLDLDQVGATTRS
                     TGHSTLGLIRCLSEVLPPQAREWIYYGATVQDLSDTWTAVVARRVGDVLERDLRRVEA
                     AALGLAREHRDVIMCGRTHGQPGLPITFGFKAAVWASELRRHRARLAEGRRRWEVVQL
                     GGALGTMEFWGTAALPLLEAFARRIGLGVPDIAWVTARDRIAEFTGLLALVSGTLGKI
                     GNEIYQLQRPEIGEVSEPFTHGQVGSITMPHKRNPELAEHLGTLARVVRSGAGVTVEG
                     LVGDHERDGRSWKAEWVVVPEACLLTGVALQLAARLLEGLVVHPARMRANLDGRGGYV
                     LSEPVMRVLADRIGKHAAQQAVYDATMAGLQRGTGLAEALAADPVVSTYLDSAEIARC
                     LDPRRALGATTAFVDRVLAAAKGPL"
     gene            1266..>2137
                     /locus_tag="VHH53_01380"
     CDS             1266..>2137
                     /locus_tag="VHH53_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2288864.1"
                     /translation="MKGLLDLPRVELCTVPTPLRPAPRLSEVLGVEVWFKRDDLTGFG
                     LGGNKVRALEYLLGDALRRGCDCLVTGAGPQSNWAMLAALAARQCGLEAHLVYYGQPV
                     PSTGNLLLAQLAGAQITFTGDPDRASVDAGSATLADTLRRAGRRPCSLPRGGATPVGA
                     LGYARASLELAEQLLAADLRPRQLWLATGSGGTQAGLLAGVRWLGLPYDVVGVTVSRT
                     VAECVHEVREVAAGVGSLLGTCAHGSGDATVLDGFLGPGYGVASSAGKEAARLVAQAE
                     GMFLDPVFGAKAMAA"
BASE COUNT          280 a          696 c          814 g          347 t
ORIGIN      
        1 ggagattctc gccgccctcg ccgaggcgca agccgaggtc gggctggtgc ccgccgacgc
       61 ggcgcgcgct atccgggact gcgcggacgt cacgctgctc gaccttgacc aggtcggcgc
      121 cacgaccagg tcgaccggcc actccaccct tggcctgatc cgttgcctga gcgaggtgct
      181 gccgccgcaa gcccgggagt ggatctatta cggggccacg gtgcaggatc tttccgacac
      241 ctggaccgcg gtggttgccc ggcgcgtcgg cgacgtgctc gaacgggacc tgcgccgcgt
      301 cgaggccgcc gccctggggc tggcccgcga gcaccgggac gtgatcatgt gcgggcgcac
      361 ccacgggcag cccgggctac cgatcacgtt cgggttcaag gcggctgtct gggcctccga
      421 gcttcgccgc caccgggccc ggctggccga ggggcggcgc cgttgggagg tagtccagct
      481 cggtggggca ctgggcacga tggagttctg gggtacagcg gcgttgccgc tgctcgaggc
      541 gttcgcccgc cgcatcggcc tgggagtccc ggacatcgct tgggtcaccg cacgggatcg
      601 gatcgctgag ttcaccgggc tactggcgct ggtgagcggg actctgggca agatcggcaa
      661 cgagatatac cagctgcagc ggcccgagat cggggaagta tccgagccat tcactcatgg
      721 gcaggtcggc agcatcacca tgccgcacaa gcgcaacccg gaactggccg agcacctggg
      781 cacgctggcc cgggtggtgc gatccggcgc aggcgtcacc gttgagggtt tggtgggcga
      841 ccacgagcgc gacggccggt catggaaggc ggagtgggtg gtggtgccgg aggcgtgcct
      901 gctcaccggg gtcgcgttgc aactggcggc ccggctgctg gagggcctcg tggtgcatcc
      961 cgcgaggatg cgggccaacc tcgacggccg tggcgggtac gtgctgtccg agcccgtgat
     1021 gcgtgtgctg gctgaccgga tcggcaagca cgcggcccag caggcggtct acgacgccac
     1081 gatggccggt ctccaacgcg gcacagggtt ggccgaggca ctcgccgccg acccggtggt
     1141 gagcacatac ctggactcgg cggagatcgc ccgatgcctg gacccgcggc gggctctcgg
     1201 ggcgaccacc gcgttcgtcg atcgggtgct ggccgcggcg aagggtccac tgtgagggcc
     1261 ccgctgtgaa gggcctgctg gacctgcccc gagtcgagct gtgcacggtg ccgacaccgc
     1321 ttcggccggc accgaggctt tcggaggtgc tcggtgtcga ggtctggttc aagcgtgacg
     1381 acctcaccgg tttcggtcta ggaggaaaca aggtccgggc gctggagtac ctgctcggcg
     1441 atgcgctgcg caggggctgc gactgcctgg tcaccggtgc cggaccgcag tcgaactggg
     1501 cgatgcttgc agcgctggcc gctcgccaat gcggcctgga agctcatctg gtctactacg
     1561 gccagccggt accgtccacc ggcaacctgc tgctggcgca gctcgccgga gcgcagatca
     1621 ccttcaccgg tgatcccgat cgagcctcgg tggatgcggg cagcgccacg ctcgccgaca
     1681 cgttgcgccg cgccggccgc aggccttgtt cgcttcctcg gggcggcgca accccggtcg
     1741 gcgcgctcgg ttacgcccga gcgagcctgg agctggccga gcagctgctc gccgccgatc
     1801 tgcggccccg gcagctgtgg cttgcgaccg ggtcgggcgg tacccaagcc gggctgcttg
     1861 ctggtgttcg ttggctgggg ctgccctatg acgtggtggg ggtgacggtc agccgcactg
     1921 tagctgagtg tgtccatgag gttcgcgagg tggcggccgg ggtggggtcg ctgttgggaa
     1981 cgtgtgcgca cggcagcggc gatgccaccg ttctcgatgg ctttctcggt ccgggctacg
     2041 gtgtggcctc ctcggcgggt aaggaggctg cccggctggt cgcccaggcg gagggcatgt
     2101 tcctcgaccc ggtgttcggt gcgaaagcga tggccgc
//
LOCUS       DATADN010000106         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_465190, whole genome shotgun sequence.
ACCESSION   DATADN010000106 DATADN010000000
VERSION     DATADN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465190"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VHH53_01385"
     CDS             complement(<1..912)
                     /locus_tag="VHH53_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX2288865.1"
                     /translation="MPDRHPDHGLLRELEPVAERLLDRHLSMAKEWLPHEYVPWRLGR
                     DFDVEPWTPDQPRLTGVAQAAFEVNLLTEDNLPSYHRALLATFGRDGAWNTWSHRWTA
                     EEGRHAIVLRDYLLVTRNIDPVALERGRMATMQQGWTPDKTALGLMAYAAFQELATRI
                     SHRNTGRYSDDPVADKIMVRVAADENLHMMFYRDIVAAALQVDPSAAVQAITEEVIGF
                     DMPGVGIQGFTGKALQMAQAGIYDLRIHHDEILWPLLRYWNLFGLEGLQPKAEQARDR
                     LAAHLEKTDRLASRFEAKRRRAAEAQER"
     gene            complement(905..1030)
                     /locus_tag="VHH53_01390"
     CDS             complement(905..1030)
                     /locus_tag="VHH53_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288866.1"
                     /translation="MQEAPLDPEDRTTDTGMLRGAAEDPGPGRSGCPVDEGGWRA"
     gene            complement(1109..>2155)
                     /locus_tag="VHH53_01395"
     CDS             complement(1109..>2155)
                     /locus_tag="VHH53_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2288867.1"
                     /translation="RVLTWIVVAALLSVILGPVVDVAERRLHLRRAVATLLVFLLFLV
                     ALAGIVTVFIRPLATEGPEFIDRLPGYVEDARAGRGPVGSLVQRYNLDQYLERNQGRL
                     QESTNRITEPALGVLRSIFSTVVALVTIFVLTFLMVLQGPKLLQSWIAALPEERQERV
                     RRVAADCSKAVTGYMTGNLLISAIAGTLTYIVLWIMGVPYKGVVALFVAFADLIPLVG
                     ATLGAIVAIAVAALHSLPAALVVLVLFVVYQQAENHLLQPVIMSRTVQLSALTVTIAI
                     LLGVELLGFLGALLAIPVAGILHVIGRDLYDGYRGHLKPDPTTGTDEIPVSQPRPPEP
                     GEPRPRDVTAATHR"
BASE COUNT          331 a          683 c          814 g          327 t
ORIGIN      
        1 acgctcttga gcttcggcgg ctcgccttcg tttggcctcg aaccggctgg ccagccggtc
       61 ggtcttctcc aggtgggccg ccagccggtc ccgggcctgc tcggccttcg gctgcaggcc
      121 ttcgaggccg aacaggttcc agtagcgcag cagcggccag aggatctcgt cgtggtggat
      181 gcgcaggtca tagatgccgg cctgggccat ctgcagcgcc ttgccggtga agccttggat
      241 gccaacgccc ggcatgtcga agccgatgac ctcctcggtg attgcctgga ccgcggccga
      301 ggggtcgacc tggagggcgg cggccacgat gtcgcggtag aacatcatgt gcaggttctc
      361 atcggcggcc acccgcacca tgatcttgtc ggccaccggg tcgtcggagt agcggccggt
      421 gttgcggtgg gagatccggg tggccagttc ctggaaggcc gcataggcca tcagccccag
      481 cgccgtcttg tcgggcgtcc agccttgctg catggtcgcc atgcggccgc gttccagggc
      541 gaccgggtcg atgttgcggg tcaccagcag atagtcgcgg agcacgatcg cgtggcggcc
      601 ctcctcggcg gtccagcggt gcgaccaggt gttccaggcc ccgtcgcggc cgaaggtggc
      661 cagcagcgcc cggtggtagc tgggcaggtt gtcctcggtg aggaggttga cctcaaaggc
      721 ggcctgggcg acaccggtca gccggggctg gtccggagtc cagggctcga catcgaagtc
      781 gcgacccagc ctccagggca cgtactcatg cggcagccac tccttggcca tggacagatg
      841 ccggtcaagc agccgctcgg cgaccggctc cagctcccga agcaggccat ggtcgggatg
      901 gcgatcaggc acgccagcct ccctcgtcaa ccggacagcc gctacggccg ggcccggggt
      961 cctcagcggc accgcggagc atgccggtgt cggtggtgcg gtcctcgggg tccagaggtg
     1021 cctcctgcat gattgcctcc tggtgggtgc ggtatgcccc agcggggagt cgggggtgtg
     1081 gggacggccg cgtggccggg ccgtcgggtt agcggtgtgt ggcggcggtg acgtcgcgag
     1141 gtcgcggctc gccgggttcg ggtggtcgag gctgcgacac cgggatctcg tcggtaccgg
     1201 tggtcgggtc gggcttgagg tggccgcggt agccgtcata gaggtcgcgg ccgatgacat
     1261 gcaggatgcc ggcgaccggg atggccagca gcgcccccag gaaccccagc agctccacgc
     1321 cgagcaggat ggcgatggtg accgtgagtg ccgacagttg gacggtgcgg gacatgatga
     1381 ccggctggag caggtggttc tcggcctgct ggtagacgac gaacaacacc agcaccacca
     1441 gggcggccgg gagcgaatgc agggcggcca cggcgatggc cacgatcgcg cccaaggtgg
     1501 ccccgaccag cggaatgaga tcggcgaagg ccacgaacag ggccaccacg cccttgtagg
     1561 gcacgcccat gatccagagc acgatgtagg tcagggtgcc ggcgatggcg ctgatgagga
     1621 ggttgccggt catgtagccg gtgacggcct tggagcagtc ggcggcgacc cggcggaccc
     1681 gctcctggcg ctcctcgggc agagccgcga tccagctctg cagcagcttg ggtccctgga
     1741 ggaccatgag gaaggtcagc acgaagatgg tgaccagcgc caccaccgtg ctgaagatcg
     1801 accgcaggac cccgagggca ggttcggtga tccggttggt gctctcctgc agccggccct
     1861 ggttgcgctc caggtactgg tcgaggttgt agcgctggac caggctgccg accggacctc
     1921 ggccggcgcg ggcgtcctcc acgtagccgg gcagccggtc gatgaactcc ggtccttcgg
     1981 tggccagggg gcggatgaag acggtcacga tgccggccag ggcgaccagg aacagcagga
     2041 agaccagcag ggtggccacc gcccggcgca ggtggaggcg tcgttcggcc acgtcgacca
     2101 ccgggcccag gatcaccgac agcagggcgg cgacgacgat ccaggtcagg acccg
//
LOCUS       DATADN010000107         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1216508, whole genome shotgun sequence.
ACCESSION   DATADN010000107 DATADN010000000
VERSION     DATADN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216508"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VHH53_01400"
     CDS             complement(<1..813)
                     /locus_tag="VHH53_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HEX2288868.1"
                     /translation="MTQDHYGKPGHGQPTETDAAHTVASGTTSETGAAHATVSDPYAT
                     SRETTEGRVYTVTGGDWDSLIDDWRHDERIVINMGPQHPSTHGVLRLVLELEGEVVTQ
                     ARSVIGYLHTGIEKNCEYRNWTQGVTFVTRADYLSPLFNEAAYCLATERLLGITAPRR
                     AQVIRVLLMELNRIGSHLVAIATGAMELGALTGMTSGFREREEVLHLLEFLTGLRMNH
                     AFIRPGGVAQDLPEGYETKIRDFIRVMDMRMPNYDKMLTGQPIWKKRLQGVGY"
     gene            complement(813..1511)
                     /locus_tag="VHH53_01405"
     CDS             complement(813..1511)
                     /locus_tag="VHH53_01405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2288869.1"
                     /translation="MTSVEKAEHEARGGVVAGRARQGMFGVRGPGDTSGYGRLLLPAY
                     VPPPAQRPYGGWFDEVTDELLAAMREGGVPAEAIQQVTVDRGEITYYLQREHLVALCR
                     TLRDDPALHFELCSSVSGVDYGPEVEQRLHVVYHLLSMTYRRRIRLEVAVDVDDAHVP
                     SVVEVYPTADWHERETWDMFGIVFDGHPALTRILMPDDWDGYPQRKDYPLGGIPVEYK
                     GAEIPPPDQRREYS"
     gene            complement(1579..>2065)
                     /gene="nuoB"
                     /locus_tag="VHH53_01410"
     CDS             complement(1579..>2065)
                     /gene="nuoB"
                     /locus_tag="VHH53_01410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX2288870.1"
                     /translation="AIEMMTTGGPRYDLGRFGMEVFRASPRQADLMIVAGRLSNKMAP
                     VLRQIYDQMPEPRWVLSMGVCASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLI
                     DAILKLHAKIMDEPLGPKRAELKAGQHTEMVASSVRYGPQKDPRPSGSGRSEVVEARK
                     Q"
BASE COUNT          330 a          691 c          652 g          392 t
ORIGIN      
        1 gtagcccaca ccctgcaacc gcttcttcca gatcggctgc cccgtgagca tcttgtcgta
       61 gttcggcatc cgcatgtcca tcacgcggat gaagtcacgg atcttggtct cgtacccctc
      121 gggcaggtcc tgcgcgacac ctccgggccg gatgaacgcg tggttcatcc gcagcccggt
      181 gaggaattcc agcaggtgca ggacctcctc gcgctcccgg aaccccgatg tcatgccggt
      241 cagcgcgccg agttccatgg caccggtagc tatcgccacc aggtgtgagc cgatccggtt
      301 gagttccatg agcagcaccc ggatcacctg cgcgcgccgc ggcgcggtga tcccgagcag
      361 cctttcggtg gccaggcaat aggctgcctc gttgaacagc ggtgacaggt agtccgcccg
      421 ggtgacaaaa gtgacaccct gggtccagtt tcggtattcg cagttcttct cgataccggt
      481 gtgcaggtag ccgatcactg agcgagcctg ggtgacgacc tcgccctcca actccaggac
      541 cagccgcagc acgccgtgcg tggagggatg ctgcgggccc atgttgatga cgatgcgttc
      601 gtcgtggcgc cagtcatcga tcagactgtc ccagtcaccg ccggtgacgg tgtacacccg
      661 accctcggtg gtctcccggg acgtggcgta ggggtctgag acggtggcgt gagcagcccc
      721 cgtctcgcta gtggttccac tggcgaccgt atgcgctgcg tccgtctcag tgggttgtcc
      781 gtggccgggt tttccgtagt ggtcttgggt catcaggagt actccctccg ctggtcgggc
      841 ggaggaatct cggctccctt gtactccacc gggatcccgc cgagcgggta gtccttgcgc
      901 tgcgggtagc cgtcccagtc gtcgggcatg agaatgcggg tcagcgctgg gtggccgtcg
      961 aagacaatgc cgaacatgtc ccacgtctcg cgctcgtgcc agtcagcggt cgggtagacc
     1021 tcgaccacgc tgggcacgtg ggcgtcatcg acgtcgacgg caacctcaag ccggatccgc
     1081 cgccggtacg tcatcgagag gaggtggtac accacatgca gccgctgctc gacctctggg
     1141 ccgtagtcca cgccggatac cgagctgcac agctcgaagt gcagcgccgg atcgtcgcgt
     1201 aaagtgcgac acagcgcgac cagatgttcc cgctgaaggt agtaggtgat ctcgccgcga
     1261 tccacggtga cctgctggat cgcctcagcg ggtactccac cctcccgcat ggcggcgagc
     1321 agctcgtcgg tgacctcgtc gaaccagccg ccgtagggtc gctgcgcagg gggcggcacg
     1381 taggccggta gtagcaaccg gccatacccg gaggtgtctc ccggaccgcg aacgccgaac
     1441 atgccctgcc gagcccggcc ggcgaccacc ccaccgcgag cctcatgctc ggccttctcg
     1501 acactcgtca tctcagcaga gctagaccgg ccgggctcga tgttcttctc ctcgctggcg
     1561 tccttgtcga tcatgcgctc actgctttcg cgcttcgacg acctcggacc gtccgctccc
     1621 cgatggtctg gggtccttct gcgggccata gcgcaccgag gaggccacca tctcggtgtg
     1681 ctggccagct ttcaactcgg cccgctttgg cccgagcggc tcgtccatga tcttggcgtg
     1741 cagtttgagg atcgcgtcga tgagcatctc cggccgtggt gggcaaccgg gcaggtatat
     1801 gtccaccggg acgacgtggt ccacgccctg cacgatcgcg tagttgttga acattccacc
     1861 gctggaggca cagactccca tcgaaagcac ccagcgcggc tcgggcatct ggtcgtagat
     1921 ctgcctcagc acgggggcca tcttgttgct cagtcgacca gccacgatca tcagatcagc
     1981 ctgtcgtggg gaggctctga acacctccat cccgaagcgg cccaggtcat atcggggccc
     2041 cccggtggtc atcatctcga tcgcg
//
LOCUS       DATADN010000108         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_163401, whole genome shotgun sequence.
ACCESSION   DATADN010000108 DATADN010000000
VERSION     DATADN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163401"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1403)
                     /locus_tag="VHH53_01415"
     CDS             complement(117..1403)
                     /locus_tag="VHH53_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2288871.1"
                     /translation="MATSTRVYAARVAGLPVFGPDGEQIGKVRDLVTALRIGPRPPRV
                     LGLVIELAIRQRIFVPMLRVTSIEPGAVALATGTVSLRRFSLRPNETLVIGEILDARV
                     HVAATGAVAVVVDAGMEQDRTRDWVITTLAVRERTGRLGRRGPVQVLGWDEVAGLTLA
                     EVSSRGQGAEQLLALYEELRAVEVANALRELPTKRRHEVVDALDDERLADVLQELSED
                     EQAEVLAHLGDERGADVLEAMNPDDAADLLGELAQTERERLLRLMAPEESAPVRRLLA
                     YSSDTAGGLMTPEPVVLTPDATVAEALARVRNPELTPALASMVFVCRPPSATPTGRYL
                     GCAHLQRLLREPPSDLVAGVLDTELARLGPQASLAEVTRYFAAYDLVCGPVVDAEGHL
                     LGAVSVDDLLDAALPDDWRQREAWPASDWTGEVTHG"
     gene            complement(1474..>2052)
                     /locus_tag="VHH53_01420"
     CDS             complement(1474..>2052)
                     /locus_tag="VHH53_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEX2288872.1"
                     /translation="PSGRSTAAAGRSDGEFAVTKVIDIPAEPAAADELPPALQPFGAV
                     PPLVTDINLSVDDQQLYVSCWGIGELKQYDVSDPAHPRETGSVQLGGIAGRAAHPAAP
                     EQRLAGGPQMVEVSRDGRRVYLTNSLYGAWDDQFYPAGVGAWLAKIDADTAGGVTLDE
                     GFFLHGDDFRGLRVHQVRLQGGDASSDSYCYR"
BASE COUNT          326 a          758 c          681 g          287 t
ORIGIN      
        1 ggaaccgggc gatccgctcc gagaagcggg cgaaggcgtc gggatccatt ctgggcgtca
       61 ggcgccgcgg cccgcggggc aggtccagcc ggcgtgccga gtactgcgaa tatgactcag
      121 ccatgcgtca cctcgccggt ccagtcggaa gccggccacg cctcccgttg ccgccagtca
      181 tcgggcagcg cggcgtccaa aagatcatcc acgctcactg cccccagcag gtggccctcg
      241 gcatccacca ctgggccgca gaccaggtcg taggcggcga agtagcgagt gacctcggcc
      301 agtgacgctt gcggtccgag cctggccagc tcagtgtcca gcacgccggc caccaggtcc
      361 gagggtggct cgcgcagcaa ccgctgcagg tgagcgcagc ccaggtagcg cccggtcggg
      421 gtcgccgaag ggggacggca gacgaacacc atgcttgcca gggcgggggt cagctccggg
      481 ttgcgcaccc gggccagcgc ctcggcaacg gtggcgtccg gggtgagcac caccggctct
      541 ggagtcatca ggccacctgc ggtgtccgag gagtaggcca gcagcctgcg cactggtgcg
      601 gactcctcag gcgccatcag ccgcagcaac cgctcccgct cggtctgggc gagctcaccg
      661 agcagatcag cggcatcgtc gggattcatc gcctccagca cgtcggcgcc ccgctcgtcg
      721 cccagatggg cgagcacttc agcctgctcg tcttcggaca actcttgcag cacgtctgcc
      781 agccgctcgt cgtccagcgc gtcgaccacc tcgtggcgac gtttggtggg tagctcgcgc
      841 aacgcgttgg ccacctccac cgcacgcagt tcctcgtaga gggcgagcaa ctgctcggca
      901 ccctgtcccc gggaggacac ctcggccagg gtcaacccgg cgacctcgtc ccatccgagc
      961 acctgaactg ggccgcgccg cccgagcctg ccagtgcgct cccgtaccgc cagcgtcgtg
     1021 atcacccagt cgcgggtgcg atcttgctcc atcccggcgt ccaccacaac cgcgacggca
     1081 ccagtggcgg ccacgtgcac ccgggcgtcc aggatttccc cgatcaccag ggtctcgttg
     1141 gggcgcagcg agaaccggcg caggctcacc gtgccggtgg ccagcgctac cgcgcccggt
     1201 tcgatcgagg tgacccgcag catcggcacg aagatccgtt gccggatcgc gagctcgatg
     1261 accagtccca gtactcgagg cggccgcggc ccgatccgca gcgcggtgac caggtcgcgg
     1321 accttgccga tctgttcgcc atcaggcccg aacaccggca ggcccgcgac ccgggctgca
     1381 taaaccctgg tcgaagtggc caccgcacga ggctagcgcc cgctcagatc ggtgagaccc
     1441 gcacctcaga ccgcattcgc gatcgcgggc ggctcagcgg tagcagtaag agtcggagga
     1501 ggcgtcgcca ccttgcagac gcacctggtg cacccgtagg ccgcggaagt catcaccgtg
     1561 caggaagaac ccttcgtcga gggtcactcc accggcggtg tcggcgtcga tcttcgccag
     1621 ccacgcgccg acgccggcgg gatagaactg atcgtcccac gcgccgtaca gcgagttggt
     1681 gagatacacc cgccgcccgt cccggctgac ctcgaccatc tgcgggccac cggccagacg
     1741 ctgctcgggt gccgccggat gcgccgcgcg cccggcgatg ccacccagct gcaccgaccc
     1801 ggtctcgcgc gggtgcgcag ggtcgctcac gtcgtactgc ttgagctcac cgatgcccca
     1861 gcacgataca tacagctgct gatcgtccac cgagaggttg atgtcggtga ccagcggcgg
     1921 caccgcgccg aacggctgca gtgcgggcgg cagctcgtcg gcggcggccg gctcggccgg
     1981 gatgtcgatc accttggtca cggcgaactc cccatccgac cgccccgccg ccgccgttga
     2041 ccggcccgaa gg
//
LOCUS       DATADN010000109         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_314426, whole genome shotgun sequence.
ACCESSION   DATADN010000109 DATADN010000000
VERSION     DATADN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314426"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..897
                     /locus_tag="VHH53_01425"
     CDS             223..897
                     /locus_tag="VHH53_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2288873.1"
                     /translation="MAGGVVHAVIFDLDGVLVDSEELWDVVRRGVAAQAGRPWPAEAT
                     RAMQGMSTVEWSTYLTDAVGVPGEPYEVAVTVIDRMAAQYHASLPLLPGAVDVVERLG
                     RRWPLGLASSSPRRLIDTVLESAGLAQRFHVSVSTEEVSAGKPSPAVYHTVVQRLGVD
                     PLQAIAIEDSSNGLRAAATAGLGVLAVPNAAFPPSEDALALADVVVDSLDEITLELVA
                     SMGRRG"
     gene            921..1655
                     /locus_tag="VHH53_01430"
     CDS             921..1655
                     /locus_tag="VHH53_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2288874.1"
                     /translation="MNEPAPWIEDRWPTDSARVRSIVLVLHGGQARSPATVRRRPQLS
                     YLRMVSFARFLHRRDGGRGMSVRILRYRYRGWDEPERHPVQDARWALAQLRDQHPAAA
                     IALLGHSMGGRVALRVADQAAVTAVCALAPWTMDDEPVWQLAGRSVLIAHGDREHMTD
                     PRLSYRFAVRAKQVTSRVCRFDVLGDGHAMLRRAHDWSELAARFVAAELGLRKPDPLI
                     EDAMRQPAPEGLRVPLPPMTRGGRWR"
BASE COUNT          327 a          648 c          703 g          338 t
ORIGIN      
        1 ccaccgcgac gaggcagtag cccctgcaga aggagtccgc aggtacatct gcacgcgagt
       61 cgtcgcttat ctcgggtgac gcggcgaccg cctcgttcgg cggcatggac gtcctcagcc
      121 aacccgagca actcgagccc gccatccgtt aggcactgac caggctggag ccgtttcaac
      181 gagaatcgaa cggaaccgtc ataggcaact cgcggaggaa tgatggccgg gggcgtggtg
      241 cacgccgtga tctttgacct tgacggcgtg ctcgtggatt cggaggagtt gtgggacgtc
      301 gtccgccgcg gtgttgccgc ccaagccggc cggccctggc ctgcggaggc gacccgagcg
      361 atgcagggca tgagcacagt ggagtggtcg acatacctta cggacgcggt gggcgtaccc
      421 ggtgaaccgt acgaggtggc ggtcaccgtg atagaccgga tggccgctca gtaccacgca
      481 tcgctacctc tgctcccggg tgcggtcgac gtggtcgagc ggctgggtcg ccgctggccg
      541 ctcgggctcg cgtcgtcctc accgcggcgg ctgatcgaca cggtgctgga gtcggctggg
      601 ttagcgcagc ggtttcacgt cagtgtgtcc acggaggagg taagcgctgg aaagccctcg
      661 cctgcggtgt atcacaccgt ggtgcagcgg ctcggggtgg atccgttgca ggcgatagcg
      721 attgaggact caagtaacgg gctgcgagca gccgctactg ccgggttggg ggtgctcgct
      781 gtgcccaatg cggcttttcc gccctccgag gatgcgcttg cgcttgccga cgtcgtcgtg
      841 gactccctcg acgagatcac cctcgagctg gtggcgtcga tgggccgccg aggctaggga
      901 caacggtgcg acttgaaccc ttgaacgaac ctgcaccctg gatcgaggac cgctggccga
      961 cggactccgc gcgggtccgt tcaatcgtgc tggtgttgca cggcggacag gcccggagcc
     1021 cggctacggt caggcggcgg ccgcagctga gctatctccg gatggtttcc ttcgctcggt
     1081 tcttgcaccg gcgtgacggc gggcgcggga tgtcggtgcg catcctgcgg tatcgctacc
     1141 ggggttggga cgagcccgag cgccaccccg tgcaggacgc gagatgggca ttggcacagc
     1201 ttcgggacca gcacccggca gcggcgatag cgctgttggg acactcgatg ggagggcggg
     1261 tggccctgcg ggtagctgac caggcggcgg tgaccgcagt atgtgcgtta gcgccatgga
     1321 ccatggacga tgagccagtg tggcagctcg ctggacggtc ggtcctcatc gcgcatggcg
     1381 accgggaaca catgaccgat ccgcggttgt cctaccggtt tgcggtgcgc gccaagcagg
     1441 tcacttcccg ggtctgccgt tttgacgtac tcggagatgg gcacgcgatg ctccgccgcg
     1501 cgcacgactg gtcggagctg gccgcccggt tcgtggcggc cgaactcgga ctgcgcaaac
     1561 ccgacccgct catcgaggac gcgatgcgcc aaccggctcc ggagggactc cgcgtgccgc
     1621 tgccgccaat gacccgaggc ggaaggtggc ggtgacgacc cgtagctccc ctcggcggca
     1681 tccccgcaga ccgacgtcgt cgcacaccgt cagcggcgac cgcggcggcg gtcccaaagc
     1741 cgcttcagta ccagaaactg caaaagcctc ttgatcacct taacctcctc agcatctcac
     1801 cgacaccccg ggcgcggccc ggcagagcct ggcaatcacc ggtatcggat acccaccaat
     1861 ccacagcttt aagcgcgcca ctccatgggc gctgcgcacc gccttgaggg atgaaaatgg
     1921 gtcggttgcc gcgctggcag ccctacgtgc gccgagttcg acagtaatga tgcacggcgg
     1981 cctttgggca gctccggtac cgaactcggc gacagc
//
LOCUS       DATADN010000110         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_314465, whole genome shotgun sequence.
ACCESSION   DATADN010000110 DATADN010000000
VERSION     DATADN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314465"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VHH53_01435"
     CDS             complement(<1..692)
                     /locus_tag="VHH53_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HEX2288875.1"
                     /translation="MSQAVPPSERPTTPTTAGPSRPPTLLEQLGGVGGLLSSVVPVVI
                     FVAVNPLIGLQPAIWASLATAVAVGVWRLVRREPLQPAVSGILGVAVCAFIAYQTGEA
                     RGFFLYGIWYSLVAGLALVVSVVVRRPLVGVLWSMLTGSGSSWRTDRRVRCGFDVATL
                     VWAVFFLARFGVQHWLYEAQETSWLGVARLAMGLPLTAVAALVTIWAIRRAVRSTRSA
                     PSRPGSPRRSRTG"
     gene            complement(689..1066)
                     /locus_tag="VHH53_01440"
     CDS             complement(689..1066)
                     /locus_tag="VHH53_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX2288876.1"
                     /translation="MSGAAPGYWRRLVHRLTSDTSELDADDLARRVEQAGARRACDCA
                     SGEAVTVLGRLRSVEHCPRAAVATLEAELFDGSEGITLVWLGRRRIPGIEPGRTVRAT
                     GRIAVRDGRKVLYNPYYELQRSS"
     gene            complement(1180..1875)
                     /locus_tag="VHH53_01445"
     CDS             complement(1180..1875)
                     /locus_tag="VHH53_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HEX2288877.1"
                     /translation="MFGRRRDQVEAQGKRRGEPAAEVPGRAPGSGSSDEQLATAGPYD
                     VAEAPDDGRIRLDLGSLRLPVPQGAELQVEVDSSGPVRAVHLMTGLGQVTLTPYAAPR
                     SGGLWREVCRELVARLRADGARVGRAAGEWGEEIEAVTAQGVMRFLAVDGPRWMLRGV
                     AVGTAEHAEARTALLREVVREAVVVRGPEPLPVRSPLPLQLPGPLAEQLKQATAGSDA
                     ATTVGDGSSARQS"
     gene            complement(1879..2328)
                     /gene="dut"
                     /locus_tag="VHH53_01450"
     CDS             complement(1879..2328)
                     /gene="dut"
                     /locus_tag="VHH53_01450"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX2288878.1"
                     /translation="MEVLLIRLDPEVPVPQYSHADDAGADLVTTQDLVLPPGERAVVG
                     TGIAIALPAGYAAFVHPRSGLAAQAGLGVVNAPGTIDAGYRGELRVCLVNHDLREPVV
                     LHRMDRIAQLVVQRVEHAAFHEVTALPGSPRGIGGYGSTGTTHQGEC"
     gene            2378..2917
                     /locus_tag="VHH53_01455"
     CDS             2378..2917
                     /locus_tag="VHH53_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HEX2288879.1"
                     /translation="MPVRRSQGRQPSLNEPTAEPVFTERLTVPWWWWPLGLGVAALLA
                     AEVHMGYPGVRAWLPYLLTVPLAGAVLIRLGQRRVRLAGGELQVGPAHVPVRYLGAVE
                     VIAPGGKQRALGPDLDPAAFVLHSAWIGPVLRIELLDPQDPTPYWIFSVRRAEELAAL
                     LRTGADQTPHESPDEHPER"
     gene            complement(2936..3238)
                     /locus_tag="VHH53_01460"
     CDS             complement(2936..3238)
                     /locus_tag="VHH53_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005513624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HEX2288880.1"
                     /translation="MATDYDAPRRSETDELTEDSLEELKARRNEAQSGAVDVDESESA
                     ENFELPGADLSGEELTVRVLPKQADEFTCSSCFLVHHRSRLAEQRSGRMVCRDCAA"
BASE COUNT          565 a         1290 c         1122 g          513 t
ORIGIN      
        1 ccggtgcggc ttcgacgtgg cgaccctggt ctggacggtg ccgagcgggt cgagcgtact
       61 gcccggcgga ttgcccagat ggtgaccaag gcggccacgg cggtcagcgg caggcccatc
      121 gccagccggg ccacgcccag ccagctggtc tcctgcgcct cgtacagcca gtgctgcacc
      181 ccgaagcggg cgaggaagaa caccgcccag accagggtcg ccacgtcgaa gccgcaccgg
      241 acccgacggt cggtgcgcca gctagacccg gagcccgtga gcatcgacca cagcacgccg
      301 accagcggac ggcgcaccac caccgacacc accaacgcca gccccgcaac caggctgtac
      361 cagatgccgt agaggaagaa tccccgcgcc tccccggttt ggtaggcgat gaaggcgcac
      421 acggctaccc cgaggatgcc ggacacggcc ggctggagag gctcccgccg gaccagccgc
      481 cacaccccca ccgccaccgc tgtagccagt gatgcccaga tcgccggttg cagcccgatc
      541 agcgggttca ccgcgacgaa gatcaccacc ggcaccacag aagacagcag accgccgacc
      601 ccgcccagct gttcgagcag ggtgggtgga cgcgagggcc ccgcggtggt cggggtggtc
      661 ggacgctcgg acggcggaac ggcttggctc atgaactccg ctgcagctcg tagtaggggt
      721 tgtaaagcac cttccggccg tcccgaacgg cgatccggcc ggtggcccga acagttcgcc
      781 cgggctcgat gccagggatt cggcgccgcc ccagccacac cagggtaata ccctcggaac
      841 cgtcgaacag ctcggcctcc agggttgcca cggccgctcg gggacagtgt tccacgctgc
      901 gcagccgacc cagaaccgtg accgcctccc ccgacgcgca gtcgcaggcg cgccgtgcac
      961 cggcctgctc gacgcgccgg gccaggtcgt cggcgtcaag ttcggaggtg tcgctggtca
     1021 gccggtggac cagtcgccgc cagtagccgg gagccgcacc gctcatgtcg atctccgacc
     1081 gcgatcgcgg gtaccccgac ggacccgctc ccaccccagg atagtccgac tccggcgcga
     1141 ttcccgctcg tcggagttga tatcgtccca ttgatgcggt cagctctgcc gggcgctgct
     1201 cccatcacca acggtggtgg cggcatcgga cccggcggtg gcctgcttga gctgctcggc
     1261 gaggggcccg ggcagctgca gcggcagcgg gctgcgcacc ggcagcggtt ccggaccacg
     1321 caccaccacg gcttcccgga cgacttcacg cagcaatgcg gtccgggcct cggcatgctc
     1381 ggcggtcccc accgcgacac cgcgcagcat ccaacgcggc ccgtccaccg ccaggaaccg
     1441 catcactccc tgtgcggtga ccgcctcgat ctcctcaccc cactcaccag ccgcccgccc
     1501 gacccgtgca ccgtcggccc gcagccgagc caccagctcc ctgcacacct cgcgccacag
     1561 cccgccgctg cgcggggcag cgtagggggt gagcgtcacc tggccaagtc cagtcatcag
     1621 atgcaccgct cgcaccgggc cggaactgtc cacttccacc tgtagttcgg cgccctgggg
     1681 taccggcagg cgcagcgagc cgagatccag cctgatccgt ccgtcgtcgg gcgcctcggc
     1741 gacgtcgtag ggccccgcag tcgccagctg ttcgtcggag gagccactgc ccggtgccct
     1801 gccgggtacc tcggcggccg gttcgccgcg acgcttgccc tgtgcttcga cttggtctct
     1861 gcgacgtcca aacactgctc agcactctcc ctgatgcgtg gtcccagtcg agccgtaacc
     1921 gccgatcccc cggggcgaac ccggcagtgc ggtcacctcg tgaaacgcgg catgctcgac
     1981 tcgctgcacg accagttgcg cgatgcggtc catccgatgc agcaccacag gctcgcggag
     2041 gtcgtggttg accaggcaaa cccgcagctc gccacgatag ccagcgtcga tcgtgccagg
     2101 tgcgttgacc actcccaacc ccgcctgcgc cgccaggccg gaccgcgggt gaacgaacgc
     2161 tgcatatccc gcgggcaagg cgatcgcgat gccggtaccc accactgcgc gctcccctgg
     2221 tggcaggacc aggtcctgag tggttaccag gtcggcgccg gcgtcgtctg catgggaata
     2281 ctggggcacc ggcacctccg gatccagccg gatcaggagt acctcgattc taaatgtctc
     2341 agccgagcac acggccggcg agactaccct cgaacccgtg cctgttcgtc gctcccaggg
     2401 tcggcagcca tccctcaatg aacccactgc cgagccagtc ttcactgaac gcctcacggt
     2461 gccgtggtgg tggtggccgc tgggattggg cgtggccgca ctgctggccg ctgaggtgca
     2521 tatggggtat ccaggagtgc gggcctggct gccgtacctg ctcaccgtgc cgctggccgg
     2581 tgcggtgctg atccggttgg ggcagcggcg ggtccggctt gccggcggcg agttgcaggt
     2641 gggcccggcg cacgtcccgg tccgctatct gggggcagtc gaagtaattg ccccgggtgg
     2701 caagcaacgc gcactgggcc ccgacctgga tccagcggcg ttcgtgctgc acagcgcgtg
     2761 gataggacca gtgctgcgga tcgaactcct cgatccgcag gaccccaccc cttactggat
     2821 cttcagcgtg cggcgcgccg aggaactcgc cgcactgctg cggaccggcg ccgaccagac
     2881 gccgcacgaa agccccgatg agcaccccga gcgctgaacg ggtgcgcccg gcccctcagg
     2941 ccgcacaatc cctgcagacc atccgcccgc tgcgttgttc ggccagcctg cttcgatgat
     3001 gcacaaggaa gcagctggag cacgtgaact cgtcggcctg cttgggcagt acccggacgg
     3061 tcaactcctc gccggacagg tcagcgccag gcaactcgaa gttctccgcg gactccgact
     3121 cgtcgacgtc gactgcaccc gactgagcct cgttgcgtct ggccttcagc tcctccaacg
     3181 agtcttcggt gagctcgtcg gtctcgctcc gccgtggagc gtcgtagtcg gtcgccatga
     3241 tctttcccac ccctgagtcg tacggatcgt gccgcagcac aacgcacagg aaccctgatt
     3301 tgtgcccgag acgttggtca cactgcgacg ctgcggatgc ggccctggca tgccgcacgt
     3361 tggctgcagt atcagccccc ggtagcgcca gagcggcgag agaatagctt accgatcaac
     3421 gcagcgcgcg gaattacccg gcgtgtcccg atacccagta ctccaagcac gtgccgtccc
     3481 cgtgggcaaa
//
LOCUS       DATADN010000111         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_465397, whole genome shotgun sequence.
ACCESSION   DATADN010000111 DATADN010000000
VERSION     DATADN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465397"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /gene="treS"
                     /locus_tag="VHH53_01465"
     CDS             <1..1277
                     /gene="treS"
                     /locus_tag="VHH53_01465"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX2288881.1"
                     /translation="TLYEEARIIFIDTEVSNWTFDPVANQHYWHRFFSHQPDLNYANP
                     AVQEAMLDVIRFWLDLGIDGFRLDAVPYLFEEEGTNCENLPRTHEFLKRCRKVVDDEY
                     PGRVLLAEANQWPADVVEYFGDAAVGGDECHMAFHFPLMRRIFMAVRRDSRFPISEIL
                     AQTPAIPSTCQWGIFLRNHDELTLEMVTDEERDYMYSEYVSDPRMRANIGIRRRLAPL
                     LDNDRNQQELFTALLLSLPGSPVLYYGDEIGMGDNIWLGDRDSVRTPMQWTPDRNAGF
                     SACDPGRIYLPVIMDPVYGYQALNVEAQSNSSQSLLNWTRRMIYVRKAHPAFGLGDFT
                     ELGSSNPSVLAYHRNHVDETGNDLVICVNNLSRFPQPVELDLSEHAGGVPVELTGGAH
                     FPPIGELPYLLTLPGHGFYWFQIILPDTERHP"
     gene            1274..1654
                     /locus_tag="VHH53_01470"
     CDS             1274..1654
                     /locus_tag="VHH53_01470"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288882.1"
                     /translation="MISPVDLLVDIADALATMLPEQRWFAGKGRPVRAVTPVRATDLG
                     GTDPRLVHAVIEVDQGEPQPDLYQLLLGVRGDLPDTLGHAWVAAIGDHAVYQAVHDPE
                     LTAVLLDLMAKGADMEGLHFAAEP"
BASE COUNT          286 a          563 c          524 g          300 t
ORIGIN      
        1 cgacgctcta cgaagaggcc cgcatcatct ttatcgacac cgaggtctcg aactggacct
       61 tcgatccggt ggccaaccag cactactggc atcggttctt ctcgcaccag ccggatctca
      121 actacgccaa cccggccgtg caggaggcga tgctcgacgt catccggttc tggctggacc
      181 tgggcatcga cgggttccgg ctggacgcgg tgccctacct gttcgaggag gagggcacca
      241 actgcgagaa cctgccgcgc acccacgagt tcctcaagcg ctgccgcaag gtcgtcgacg
      301 acgagtaccc gggccgggtg ctgctggccg aggccaacca gtggccggcc gacgtggtgg
      361 agtacttcgg cgacgcggcg gtcggcggcg acgagtgtca catggccttc cacttcccgc
      421 tgatgcggcg gatcttcatg gcggtgcgcc gcgattcccg gtttccgatc tcggagattc
      481 tggcccagac gccggccatc ccgtccacct gccaatgggg aatattcctg cgcaaccacg
      541 acgagctgac cctggagatg gtcaccgacg aggagcgcga ctacatgtac agcgagtacg
      601 tgtccgaccc ccggatgcgg gccaacattg gcatcaggcg gcgactggcg ccattgttgg
      661 acaacgaccg caatcagcaa gagctcttta cggcgttgtt gctgtcgctg cccggttccc
      721 ccgtgctcta ctacggtgac gagatcggga tgggggacaa catctggctt ggtgatcggg
      781 attcggtgcg cacgccgatg cagtggaccc cggaccggaa cgcgggtttc tccgcgtgcg
      841 acccgggtcg tatctacctg ccggtgatca tggatccggt atacgggtac caggcgctca
      901 atgtcgaggc gcagtcgaac tcgtcccagt cgctgctgaa ctggactcgg cgcatgatct
      961 acgtgcgcaa ggcacacccg gctttcgggc taggcgactt caccgagctc ggctcgtcca
     1021 accccagcgt gctggcctac caccgcaacc acgtcgatga gaccggcaac gacttggtca
     1081 tctgtgtgaa caacctgtcc cgcttcccgc agccggtgga actcgacctg tccgaacacg
     1141 ccggtggagt cccggtcgag ctgaccggcg gtgcgcattt cccgccgatc ggcgagctgc
     1201 cctacctgct cacgctgccg ggacacggct tctactggtt ccagatcatc cttcccgaca
     1261 ctgagcgcca cccatgatca gtccagtcga tctgctcgtc gatatcgccg acgcgctggc
     1321 gacgatgctt cccgaacagc gctggttcgc cggcaagggc cgcccggtgc gagctgtcac
     1381 cccggtgcgt gccaccgatc tgggcgggac cgatccgcgg ttggtgcacg cagtcatcga
     1441 ggtcgaccaa ggtgagccgc agccggatct ttaccagctg ctgctcggag tccgtggcga
     1501 cctccccgac accctgggcc acgcctgggt ggccgccatc ggagaccacg cggtgtacca
     1561 ggcggtgcac gatcccgagc tcaccgccgt gctgctagat ctgatggcca agggcgccga
     1621 catggagggt ctgcactttg ccgctgagcc atgagtcacg ctggacaccg aac
//
LOCUS       DATADN010000112         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1068405, whole genome shotgun sequence.
ACCESSION   DATADN010000112 DATADN010000000
VERSION     DATADN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068405"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..313
                     /locus_tag="VHH53_01475"
     CDS             188..313
                     /locus_tag="VHH53_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288883.1"
                     /translation="MEACGSAIEEQFVLVSPQHPGDSDDALPRQPRVRWPDGGDA"
     gene            407..781
                     /locus_tag="VHH53_01480"
     CDS             407..781
                     /locus_tag="VHH53_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2288884.1"
                     /translation="MAGIARFSLVALDCRDPRALAEFYAAITGWEPDRDDDDWVQLRG
                     DGGATLAFQRAPGHQPPVWPSDQHPQQAHLDFDVPDLDIGEEHVLALGARKADVQPGT
                     TFRVYLDPAGHPFCLVLDEQPA"
     gene            complement(1216..1374)
                     /locus_tag="VHH53_01485"
     CDS             complement(1216..1374)
                     /locus_tag="VHH53_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HEX2288885.1"
                     /translation="MIILGIVLVVLGLLFPQVNILVTIGIILIVVGAILALLGGFGRT
                     VGPRRHYW"
BASE COUNT          304 a          551 c          535 g          306 t
ORIGIN      
        1 gaacacggaa gacgccctgc ggctaccgca ggctactcga acagcgtggt cgtccggtgt
       61 ggcgatccga tcatgcccga tgggccggcg ttgacgctgg ctgatggtgt gcaaccacga
      121 gtacaccacc gagccgtaac cgctagcagc gcggcctgtc aagctcttcg ccaccgtccc
      181 ggtcggggtg gaggcctgcg gctcggcgat cgaggagcag ttcgtgctgg tgtccccgca
      241 gcaccccggc gactccgatg acgccttgcc ccgacaaccc cgcgtccgct ggcccgacgg
      301 cggcgatgcg taacccgccc atccgtcgcc gtggtctacc gcgacggtgg cgaccacatc
      361 gggttctgag ccaagactgt cggggtccgc cagtactgtt cgcagcatgg ctggtattgc
      421 acggttcagt ctggtagcgc tcgattgccg tgacccgcgt gccctggcgg agttctacgc
      481 tgcgatcacg ggttgggagc ccgaccgtga cgacgacgac tgggtgcagc tgcgcggtga
      541 tggcggcgct acgttggctt tccagcgtgc tcctggccac caacctccgg tgtggccgag
      601 tgaccagcac ccgcagcaag ctcacctgga cttcgatgtg ccggatctcg atatcggtga
      661 ggagcacgtg ctggcgttgg gtgcccgcaa ggctgatgtg cagcccggga cgaccttccg
      721 ggtttacctg gacccggcag ggcacccgtt ctgcctcgtg cttgatgagc agccggcgta
      781 gtgccgctgg caccttgtcc cttatccatc tgatgacggc gatgctgacc cgcatggaga
      841 cggagcgtca caagcggtcc gtgttatccc catgcaggcg acgcgctccc gacccgccac
      901 atgtccacta cggctttcga cgaagctatt tacgttttca gcgcgatgct cactctttat
      961 ttgaattctc ttaaccctct agcgggcagg ggaaccggcg acatctcatc tatccgccat
     1021 tgacccaccc gcccgcgcgg aagcgaccgg gtcattgccc cggcgaggcg caacagaccc
     1081 gtagttcgtc gcgcttcaag cggatggcga gggcgcggtg gtatgagcac ggcgggcgag
     1141 acagcgcatc ccgacagtta ccgctcagca tgcaccgaca cgcccgcgcg caggctcgaa
     1201 aagctgccga gccagttacc aatagtggcg tcgggggccg acagtgcggc cgaacccgcc
     1261 gagcagagcc aggatcgctc cgaccacgat caagatgatt ccgatcgtga cgagaatatt
     1321 cacctgcggg aacagaaggc caagcaccac caagacgata ccgagaatga tcatgattcc
     1381 tcctgaatcc ctggtcaacc tggcccggac ctgtaccgga cgaggcagac ctgttcactc
     1441 tcagcttaac cccgtcgaac gcgtttgcaa acgtgctgag cttgtcgccg ccgccacttg
     1501 caggtcgtgg ccgcagtatc acccagatgc cgcgctgcgc tgccgctgta cggaagctgc
     1561 atcctcacgc cgccggctgg gcccgggctg gagcttcacc atgggctgaa cgagtgtatt
     1621 gggaatggtc gcccacgctg tggggtatgt cacaaagggg gtatggaggc tggtatccag
     1681 atatcaacga cggagg
//
LOCUS       DATADN010000113         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1369587, whole genome shotgun sequence.
ACCESSION   DATADN010000113 DATADN010000000
VERSION     DATADN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369587"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VHH53_01490"
     CDS             complement(<1..1439)
                     /locus_tag="VHH53_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2288886.1"
                     /translation="MVSALVLGLTGYGWSAFQSLQKGLSTTDVIGFGASDSAIDILLV
                     GMDSRTDAQDNPLPADVLSRLRAGNNEGELTDTMILVRIPNDGMHAVAISLPRDLYVQ
                     LPEGYGKHKLNSAFTRAKSATAAQLVEQGTDPATVRNESIAAGRKLLVETVQDLTGVG
                     IDHYAEINLLGFSLLTEAVGGVEVCLREPVNDKFSGANFPAGPQVISGPDALAFVRQR
                     HGLPGGDLDRIVRQQAYLAALADKVLSAGTLASPTRIRQLIDATQQSLVLDEDLDLLD
                     FAARMQGLAAGNVMFKTVPVLGDGDSESDGAVLKVDPQAVQAFVAQSIDAHPVPDLTP
                     GSPARAVVTVDVLNASGVTGLATSVSEQLAGQGFRQGAVGNSPARATSVVRYPSGEHD
                     AGAAVADTLGGLLVEEGSNLAPGTVQVYLGRDYSGPEVPRIGGASLLRLGGTTASNAL
                     RPVAYTALPPLAAVAPRPPMVSSDVPCVS"
     gene            1814..>2403
                     /locus_tag="VHH53_01495"
     CDS             1814..>2403
                     /locus_tag="VHH53_01495"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX2288887.1"
                     /translation="MRLLVTGGAGFIGSTFVRRVLGGAEPALAGAEVVVLDKLTYAGN
                     AENLAPVATDPALHFVKGDITDALLVADLMIGVDAVVHFAAESHVDRSILGAAPFVVT
                     NVVGTHALLQAALEAGVSTFVHVSTDEVYGSIESGSWTEDHLLEPNSPYSASKAGSDL
                     LARAYAKTYGLDVSISRCSNNYGPYHYPEKVIPLFIT"
BASE COUNT          362 a          796 c          819 g          426 t
ORIGIN      
        1 caggaaacgc acggcacgtc gctgcttacc atcggcggtc tcggtgccac agctgcgagt
       61 gggggtaggg cggtatacgc cactggacgc agggcattgc tggcggtcgt cccgcccagt
      121 cgcagcaaag atgcgccgcc aatccgcggt acttccgggc cggagtagtc ccggccgagg
      181 tacacctgca cggtgccggg cgccaggttc gagccctctt caaccagcag gccgcccagc
      241 gtgtcggcta ccgctgcgcc ggcgtcgtgc tcgccggacg ggtagcgcac cacgctggta
      301 gctcgggcgg gactgttgcc gaccgcgcct tggcggaacc cttgcccggc cagttgctcg
      361 gagaccgaag tggcgagccc ggtcacgccc gaggcgttga gcacgtcgac ggtgacgacc
      421 gctctggctg gggagccggg tgtgagatcc ggcacgggat gggcgtcgat ggactgggcg
      481 acgaacgcct gcacggcctg cggatcgacc ttgagcaccg cgccgtcgga ctcgctgtcg
      541 ccatcaccca acaccggcac ggtcttgaac atcacgttgc ctgcggccag cccttgcatc
      601 cgggcggcga agtcaagcag gtcgaggtcc tcgtcaagca ccagcgattg ctgggtcgcg
      661 tcgatcagct ggcggatccg agtcgggctg gccagcgtcc cagccgagag caccttgtcg
      721 gccagcgcgg cgaggtacgc ctgctggcgc acgatgcggt ctaggtcgcc accgggcagc
      781 ccatgccgtt gccggacgaa cgccagcgcg tccggtcccg agatcacctg cgggccggcg
      841 gggaagttcg cgccggagaa tttgtcgttg accggttccc ggaggcatac ctcaaccccg
      901 ccgacggcct cggtgagcag cgagaagccg agcaggttga tctctgcgta gtggtcaatg
      961 cccactccag tgagatcctg gacggtctcc accagcagct ttcggcccgc cgcgatggac
     1021 tcgttgcgga ccgtggccgg atcggttcct tgctcgacga gctgcgctgc ggtggcgctc
     1081 ttggcgcggg tgaacgcgga attgagcttg tgcttgccgt acccctcggg tagctgcacg
     1141 tacagatccc tcggcagcga gatcgctacc gcgtgcatgc cgtcgttggg aatccgcacc
     1201 aggatcatgg tgtcggtcag ctcaccctcg ttgttgccgg cacgcagcct ggacagcacg
     1261 tcggcgggca gcgggttgtc ctgcgcgtcc gtgcggctgt ccatgcctac cagcaggatg
     1321 tcgatggctg agtccgatgc gccgaagccg atcacatccg tggtgctcaa acctttctgc
     1381 aagctctgga aggccgacca cccgtacccg gtcaggccca gcaccagtgc agaaaccatc
     1441 gcaagcacca gcttggcccc gccgaacccg tacttcgggc gtcgccggga agctggtatc
     1501 ccgccacgtg cgcgtggcga cgacatggtc gtggggttgc gggccgtccg gggctctcgt
     1561 agggattgtt tccgggattg cggatcaccg tctggtccgc gccgccgacc cggccgttcg
     1621 ggttgcctgg gggaactccc gggccggggt ggcctgtctt ccacgcatcc ccctcctgcg
     1681 ttttgcggct ggtgcggcgg caaggccagg ctatccgctt gcctagtgca gacgtgtgaa
     1741 cgcctggtcg ggttgccatg gcacactcgg atcgcctctg tctgactgca agccagattg
     1801 ggggagtgaa ccgatgcggc ttctggttac cggcggtgct ggattcatcg ggtccacgtt
     1861 cgtgcgccgg gttctgggtg gggcggaacc tgcactggcc ggcgccgaag tggtggtcct
     1921 cgacaagctg acctacgcag gcaatgcgga gaacctggcg ccggtcgcga ccgatccggc
     1981 gctgcatttc gtcaagggag acatcaccga cgcattgttg gtggccgatc tgatgatcgg
     2041 tgtcgacgcg gtggtgcact tcgccgcgga gtcgcacgtg gaccgctcga tccttggtgc
     2101 cgccccgttc gtggtgacca acgtggtagg cactcacgcc ctgctgcagg ccgcgctgga
     2161 ggctggcgtg tcgacgtttg tgcacgtctc caccgacgag gtgtatggct cgatcgagtc
     2221 gggctcctgg accgaggacc acctgctgga gcccaactcg ccgtactcgg cgtccaaggc
     2281 cggcagcgac ctgctcgccc gggcctacgc gaagacctac ggcctggacg tctccatctc
     2341 ccgctgcagc aacaactacg gcccgtacca ctaccccgag aaggtcatcc cgctgttcat
     2401 cac
//
LOCUS       DATADN010000114         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_766908, whole genome shotgun sequence.
ACCESSION   DATADN010000114 DATADN010000000
VERSION     DATADN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766908"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1340
                     /locus_tag="VHH53_01500"
     CDS             498..1340
                     /locus_tag="VHH53_01500"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2288888.1"
                     /translation="MTWALERSRPLSVAGKVVSRPMAKLAGTGPVKDLLSGTWLGHPA
                     HPLLTDIPIGAWTSAFLLDLVGIFSGTSRRGADTLVAVGVVAALPTAASGLSDLSDEV
                     DDSILSVGAAHAIGNVVALGLFTASCAARLRRKRARGIALSVLGTGVLVASGLLGGHL
                     SYRKGLGVDHTVFEEPIGDWTAVLDASELADGTPQQVTVAGNEVLLYRTNSAVHALAN
                     RCSHRGGPLHEGKIEGQEIVCPWHSSRFRIQDGSVVRGPAVAPQPCYEVRVNQGKIEI
                     RSVA"
     gene            complement(1433..>1666)
                     /locus_tag="VHH53_01505"
     CDS             complement(1433..>1666)
                     /locus_tag="VHH53_01505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX2288889.1"
                     /translation="MFLVAQRMAAALRRSGLRCEGVNLFLADGAAAGQEVFHVHLHVF
                     PRYAGDYFRLDSGQRRAPRRDLDEIAARVRSEL"
BASE COUNT          308 a          533 c          537 g          288 t
ORIGIN      
        1 cgcgacctca ttcgcctgcg ccgcaactgg tacgacacga cgcgcgggct tcgtggagtc
       61 atgtggcggt ccaccatgtc aacgatgacg acaacgtcct ggcctaccac cgctggaatc
      121 acggcggacc gcgtgatgac gtcgtggtga tactgaacct ggcgaaccgt agctacgacg
      181 cctactgggt cggcctgccg cgcaagggca ggtggcgagt aaggctcaac agcgactgga
      241 cgggatatag cgacgacttc ggtacgcagg aagctacgac acgacgtcgg aagaccggcc
      301 ggtggacggg atgccgtgca gtgccagcat cgggctcggg ccctacaccg cactggtgct
      361 ctcgcaggac gagtgacaag cgccctgatt ggcctcctgc accgtgcgga gtgctacatt
      421 ccgtggttga atgcggcatg gtgagtgact ctgaacagcg gccgccggca gctcgactca
      481 atcgtcaact cgaatcgttg acctgggcgc tcgagcgttc tcgcccgctg agcgtcgctg
      541 gcaaggtggt cagtaggccg atggccaagc tggccggaac cggtccagtg aaagatctgc
      601 tgagcggcac gtggctgggc catccggcgc atcctttgct caccgatatc cccatcggtg
      661 cgtggaccag tgcgtttctt ctagatcttg tgggaatctt cagtggcacc tctcggcgag
      721 gggccgacac gctggtggcg gtgggcgtcg tcgcggccct acccacagcg gcctccggtc
      781 tgtccgactt gtcggatgag gtcgacgact ccatcctttc ggtcggtgcc gcccatgcca
      841 tcggcaacgt cgtggcgctg ggcctgttca ccgcctcatg tgccgcgcgg ctccggcgca
      901 aacgagcccg aggcattgcg ctatccgtgc tcggtaccgg tgtgctcgtt gcctcgggat
      961 tgctcggtgg gcacctgtcc taccgcaagg gtctgggcgt cgaccacacc gttttcgaag
     1021 agcccatcgg agactggact gctgtactgg acgcctccga gctggccgat ggaactccgc
     1081 agcaggtcac cgtggcaggc aacgaggtgc tgctctatcg gacaaacagc gcagtccacg
     1141 cgctggctaa ccgctgcagt catcgtggcg gaccgctcca cgagggcaag atcgaaggtc
     1201 aggaaatcgt ctgcccgtgg cactccagcc gtttccgcat ccaagacgga tccgttgtcc
     1261 ggggaccggc agttgccccg cagccctgct acgaagtgcg ggttaaccaa ggaaagatcg
     1321 aaataaggtc ggtggcgtga atgcggcagc atgacagacg tgcctgacgg cacccataga
     1381 tcccccagcc cgtcagcgac ccgccagtgc gttcatcgct gatcgctgag cgtcacaact
     1441 cagagcggac ccgggccgcg atctcgtcaa gatctctgcg gggcgcccgc cgctgcccag
     1501 aatcgaggcg gaagtagtcc cccgcataac ggggaaacac gtgcagatgg acgtgaaaga
     1561 cttcctggcc cgcagccgcg ccatcagcga gaaacaggtt cactccctcg cagcgcagac
     1621 cgctgcgccg aagagccgcg gccatgcgct gagcgaccag gaacat
//
LOCUS       DATADN010000115         1740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_13138, whole genome shotgun sequence.
ACCESSION   DATADN010000115 DATADN010000000
VERSION     DATADN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13138"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VHH53_01510"
     CDS             complement(<1..962)
                     /locus_tag="VHH53_01510"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX2288890.1"
                     /translation="MPGQLCRAGGLQHGAGRRAVGSLAVGWPLDWLDEAAAVRRTTGL
                     RRALRPRPATESVIDLASNDYLGLARDPRVVEGAVDAARTWGAGSTGSRLVTGSTELH
                     AQLEDELAGFCGAAAALVFSSGYTANLGVVTALSGPGALVVSDAASHASLVDACRLSR
                     ARVVVTPPADVAAVDTALATRSEPRALVVTDSVCGINGDLAPLAELHRVCRDNGALLL
                     VDEAHGFGVCGPGGRGLLAECALAGESDVVATVSLSKSLGGQGGAVVGCRSVIDHLID
                     SARSFIFDTGLAPPAAGAALAALRVLRAEPELPGRVLDAAAAIAA"
     gene            complement(962..1609)
                     /locus_tag="VHH53_01515"
     CDS             complement(962..1609)
                     /locus_tag="VHH53_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX2288891.1"
                     /translation="MREYLKQLVDGQRPRALFLTCADSRVLPNVLTSSGPGDLFTVRN
                     LGNLVPSPGHKSDSSVAASLEYAVHHLQVPSILVCGHSGCAAMHGLLSNRAVTGSLGR
                     WLRWGLPSLAALRSGHPVGVAAAAEGRDEVDQLSMVNVACQVDMLSMHPAVRDAVASG
                     RVQVAGLFLDIRTARLLLLDSAGDAAARFVPVPDDQLPGGLVLPDPAGQTPMPGQ"
     gene            1598..1740
                     /locus_tag="VHH53_01520"
                     /pseudo
     CDS             1598..1740
                     /locus_tag="VHH53_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
BASE COUNT          302 a          634 c          587 g          217 t
ORIGIN      
        1 gcggcgatcg cggcggcggc atcgagcacg cggccgggca gctcaggctc ggcgcgcagg
       61 acgcgcaatg cagccagcgc cgcgccggct gctggcgggg ccaggccggt gtcgaagatg
      121 aagctgcgcg ccgaatcgat gagatggtcg atcactgagc ggcaacccac tacggcgccg
      181 ccctgcccgc cgagcgattt cgacagcgag accgtagcca ccacatcgga ctcaccggcc
      241 agcgcgcatt cagcgagcag ccctcgcccg ccgggcccgc acaccccgaa accgtgcgcc
      301 tcatcgacca gcagtaacgc cccattgtcc cggcacaccc ggtgtaattc ggccagtggc
      361 gcgaggtcac cgttgatgcc gcacaccgaa tcggtgacga ccaaggcgcg cggttcggac
      421 cgggtggcca gcgcagtgtc caccgccgcg acgtcagccg gcggggttac cacgacccgg
      481 gcccgcgaca gccggcaggc gtcgaccagt gaggcgtggc tggcggcgtc ggacaccacc
      541 agcgcgcccg ggcctgacag cgccgtcacc acgccgaggt tggcggtgta acccgaggag
      601 aacaccaggg ccgccgccgc cccacagaac ccggcgagct catcctccag ctgggcgtgc
      661 aactcagtgg atccggtgac aaggcgcgat ccagtcgacc cggcgcccca ggtgcgcgcc
      721 gcgtccaccg cgccctcgac gacccgagga tccctggcca agcccaggta atcgttggaa
      781 gcgagatcga tcaccgactc ggtcgccggc cgcggccgta gcgcgcgccg tagcccggtg
      841 gtgcgacgaa cagctgctgc ctcgtccagc caatccaggg gccaacccac ggccaacgag
      901 cctacggcgc gccgaccggc tccgtgctgc agaccgccgg cccggcacaa ctggccaggc
      961 atcactgccc tggcatcggt gtctggccgg ctgggtccgg aagcaccagg ccaccgggca
     1021 gttgatcgtc aggcaccggg acgaatcgag cagctgcgtc cccggcggag tcgaggagca
     1081 gcagccgggc ggtgcggatg tccaggaaca agcccgcgac ctgaacccgg ccgctggcga
     1141 cggcatcgcg cactgcagga tgcatgctga gcatatctac ctggcaggcc acgttgacca
     1201 tggagagctg gtccacctca tcccggccct ccgcggctgc cgcaacacca accgggtgcc
     1261 cgctgcgcag ggccgccaga cttggcaggc cccaccgcag ccaccggccc agggacccgg
     1321 tgacggccct gttactgagc aacccgtgca tcgctgcgca gccggaatgc ccacagacca
     1381 ggattgaggg cacctggagg tggtgcaccg cgtactccag cgacgcggcg accgacgagt
     1441 cggacttgtg cccgggcgag ggcaccaggt tgccgagatt gcgtaccgtg aacaggtcgc
     1501 caggcccgct gctggtgagg acgttcggta gcacccgcga gtcagcgcag gtcaggaaca
     1561 gtgcccgggg tcgttgcccg tccaccaact gtttcaggta ctcccgcacc tggaacagtt
     1621 ggtggacggg caacgtcccc gggcgctgtt cctgacctgc gctgactcgc gggtgctacc
     1681 gaacgtgctg accagcagcg ggcccggtga cctgttcacg gtacgcaatc tcggcaacct
//
LOCUS       DATADN010000116         4202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1370114, whole genome shotgun sequence.
ACCESSION   DATADN010000116 DATADN010000000
VERSION     DATADN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370114"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..461
                     /locus_tag="VHH53_01525"
     CDS             30..461
                     /locus_tag="VHH53_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2288892.1"
                     /translation="MATGIQVVFDCADPDSLARFWAEAIGYKLEDPPTGYGSWEHWAR
                     EQGIPQERWNDASAVDPDNRGPRLYFQRVPEAKTTKNRVHLDLSASGAHGTPLAERRR
                     RIDAEVERLLGAGATRLGTLEGAGGYVVNMADPEGNEFDVQ"
     gene            704..1108
                     /locus_tag="VHH53_01530"
     CDS             704..1108
                     /locus_tag="VHH53_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288893.1"
                     /translation="MDRPSSNQGPEASWPWTWPLGGDVTQRIGTWWIKSLSDQTGFIN
                     INNVRAGDPELERRIIEDVASYGRQLGRMMEALDVVIGHLRLGDSKNLTADERRALQD
                     FSDLVRQVQTLKGDTSRPRLTAAELDRMLDDL"
     gene            1565..2227
                     /locus_tag="VHH53_01535"
     CDS             1565..2227
                     /locus_tag="VHH53_01535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2288894.1"
                     /translation="MADSRAWWNSAAREDASWYIATASDPFFERGRRDADELIAFCGF
                     QPSTGKTLLEIGAGAGRMTHRFAELYGRVIALDVSDEMLTLGRSNLAGVGNVEWVLGS
                     GADLAGIPDRSIDDVFSYITLQHVPTSAAVLGYLEEAGRVLRPGGLGALQVRHPGPLP
                     WAVDLAGHLVHAAQGRRVWSAAWRGTRVPRHRLFQAASRPGIRVELRPRGHRHLWVLL
                     RR"
     gene            2973..3248
                     /locus_tag="VHH53_01540"
     CDS             2973..3248
                     /locus_tag="VHH53_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288895.1"
                     /translation="MPRSHDLLTPTEHEAAQLLVTLAWRLDGGQLVNADQLLARLCSI
                     PTNDVAMAGIVLLREIARSVAPVLQRPPIEVLDHYRAWLAGQPGRSG"
     gene            complement(3274..>4202)
                     /locus_tag="VHH53_01545"
     CDS             complement(3274..>4202)
                     /locus_tag="VHH53_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288896.1"
                     /translation="FVQTAPPTEAPSAFPFELSKSSRHTAGMVVIGACVLVVLAGLVA
                     VVRWGGLAVESPPATAPGDPPPVGLVVRRYLWAVNLAVIAGIGAGVMAAGTGGRLVMR
                     LLAVTAGHGAQGRITEADQVVGRISAGGTVGFVVFTALFFGTATGAAYLLVRRWLPAG
                     RAGGLVYGGLLLLVAGTRLEPLREGNVDFDLVGPGWVSVLAFSALVLFHGTLVAALAG
                     RVSRAVPLPALEPRAIAAHAPLLLLTPLLPVVGTLAVVGGLVVLATRARPVLAAWHAH
                     RLVPAGQVVLALVALVALPGFVSAVASILGRP"
BASE COUNT          720 a         1487 c         1427 g          568 t
ORIGIN      
        1 gtgagccctc gcgagcaagg aggtggttga tggcaaccgg tatccaggtg gtattcgact
       61 gtgccgaccc ggactcgctg gcgcggttct gggccgaggc gatcggctac aagctggagg
      121 acccacccac cggctatggg agctgggagc actgggcgcg cgaacaaggc atcccccagg
      181 agcgctggaa cgacgccagc gccgtcgatc ccgacaaccg cggcccgcgc ctgtacttcc
      241 agcgggtccc cgaggccaag accacgaaga accgcgtgca cctggacctc agcgctagcg
      301 gcgcccatgg cacgccgctg gccgagcggc gccgccgcat cgacgcggag gtggagcggc
      361 tgctgggggc gggcgcgacc aggctgggga cgctggaggg ggccgggggg tatgtggtca
      421 acatggccga ccccgagggc aacgagttcg acgttcagtg accctgaccg ggacgctggc
      481 ggggaggcgt gggtgcccgc gtgccagcca tgtgccacat caggcggaca agcccggcat
      541 cggacggaca accacggtca ctgacatgtc gccgatgacc tggccgccta gggcgctagc
      601 tgctggaaca catcccgcga acatgcctga taagcatgtg ctcctcggcg ccgtcgatat
      661 agtgaaaatc tagggccgca gaccagtaag aggagcacct gcgatggatc gaccgtcatc
      721 gaaccagggg ccggaggcct cgtggccatg gacctggccg ctgggcgggg acgtcaccca
      781 gaggatcggg acctggtgga tcaagtcgct cagtgatcag actggtttca tcaacatcaa
      841 caacgtccgc gcaggcgatc ccgagctcga gcggcgcatc atcgaagacg ttgccagcta
      901 cgggcgccag ctgggccgga tgatggaagc gctcgatgtg gtcattggcc acctgcgact
      961 tggcgacagc aagaacctca ccgccgatga gcggcgcgcg ctgcaggact tctccgacct
     1021 cgtccggcag gtgcagacgc tcaagggtga caccagtcga ccgcggctca cggcggccga
     1081 gttggaccgg atgcttgatg acctctaggc gctcaagcac caagacccgc ccgcctacca
     1141 gcgcatcgct gcccgactcc gcgatgtcat ctctgactga gctgcgagcg cctgcacagt
     1201 atgtagctga ttactcccga aatcggtcgc acggccaggc ttgcgcttct gccggtgttc
     1261 tgcccaaccg gtccggaccc gaccgaacgg agccggatca gcccgaacgc aaggtccgcc
     1321 tccaagtcct ctggccaggg tagatccggc tcaggcggac gatcccggac ggacccggat
     1381 gcgctgcacc cggctgaaac cgcaaggtca gcggttcgat cccgtccctg cccaccacat
     1441 cggcccacgt caacggggct ggctcatctg gcttccccca gcgttcgcca ggttgttccc
     1501 ttacgcggat tctcgtagag tccgcccgct aatgacgctc gtcgggaatg gggcgaggga
     1561 tccagtggcc gactccagag cctggtggaa cagcgccgcg cgtgaagacg ccagctggta
     1621 catcgccacg gcctccgacc ccttcttcga gcgcggccgc cgggacgccg acgagctcat
     1681 cgccttctgc gggttccagc cgtccacggg caagacgttg ctggagatcg gcgccggggc
     1741 cggtcggatg actcaccgct tcgccgagtt gtacggacgg gtgatcgccc tcgacgtgtc
     1801 tgacgagatg ctgacgctcg gccgcagcaa cctcgccggc gtgggcaacg tggagtgggt
     1861 gctcggttcc ggtgccgacc ttgccgggat ccccgaccgt tcaatcgacg acgtcttctc
     1921 ctacatcact ctgcagcacg tcccaacgag tgcggcggtc cttggctacc tggaggaggc
     1981 cggccgcgtg ctgagaccag gtggccttgg cgcgctgcag gtgcgccatc cgggtccgct
     2041 gccgtgggcg gtcgacctcg ccggtcacct tgtgcacgcc gcccagggtc gccgggtgtg
     2101 gtcggctgcc tggcgcggca cccgtgtccc gaggcaccgg ctcttccagg cggcgtccag
     2161 acccgggatc cgggtcgagc tacgaccgcg tgggcatcgg cacctgtggg ttttactacg
     2221 ccgctgatcc gtgcgatcaa ccaccggacg cgatggccct gactgcccct tcggagatgc
     2281 ttgaggcggt gtcgaggccg gtcgaggacg acggcggcgg ccacgaccct cgccgcttcc
     2341 tatggtggcg caacggatgg cgtagggcac tccgcgggag aaggcaacca gcacacatcg
     2401 gggcagggtc gcctggcggg ccacggcgag cacgtcgccg gcgtggtcat cgagggtgta
     2461 gccggaggcg ggagcgtcgc ttcggcctcg tccgcgcggg ctgatggcca cacagcggcg
     2521 aggcaagagc cgacccatca gctcggtgta ctccccggcc ggctccgaga gacggggcac
     2581 gatgacgatg ggcaactcat cgccaccatc ggcgtgagcc cggccgtacc ctccatcgcc
     2641 gctatggagc agcgacgagc ctgacccgac caggctccgg ccgatccgtg ccgggtccgt
     2701 atcacagcgg tcaagcagtg accgctgcgg gctgagcggc cgggaccggt acgggaagct
     2761 tcaggtcacc agacctgcac gtacatgagc tcggctgctg aaacaggcgc ggaagcggct
     2821 tcgaatcccg cctcccgctc ttgtctggct cgggttgacc ctgaggaaca gatcccggtg
     2881 aagggcgctg atgctgatga gatcagtcgc cgaccctggc atagcatgcg cagatcaggc
     2941 cggacagcgg ctccaggcca ggagaacaag gcatgcccag gtcgcatgac ctcctcaccc
     3001 cgaccgagca cgaggcagcc cagcttctcg tcacccttgc atggcggttg gacgggggac
     3061 agctcgtcaa cgccgaccag ttgctggcca gactttgcag catcccgacc aacgacgtcg
     3121 ccatggccgg catcgtgctg ctccgggaaa tcgcgcgcag cgtcgcaccc gtgctccaac
     3181 ggccacccat cgaagtactc gatcactacc gggcatggct cgccggccaa ccggggagat
     3241 ccggctaagc gcgcctccat ctcgagacac cagctacggc cggccgagga tgctggcgac
     3301 ggccgagacg aagccgggca gcgccaccag ggcgaccagg gccaggacca cctggccggc
     3361 cgggacgagc cggtgggcgt gccaggcggc cagcaccggc cgggccctgg tggccaggac
     3421 caccagcccg ccgaccacgg ccagggtgcc gaccaccggc agcagcgggg tgagcagcag
     3481 cagcggggcg tgggcggcga tggcccgcgg ctcgagagcc ggcagcggga cggcccggct
     3541 gacccggccg gccagggcgg cgaccagcgt gccgtggaac agcaccaggg ccgagaacgc
     3601 cagcaccgac acccagcccg gtcccaccag gtcgaagtcg acgttgccct cccggagcgg
     3661 ctccagccgg gtcccggcca ccaggagcag cagccccccg tagaccagcc ccccggcccg
     3721 tcccgcgggc agccagcgcc gcaccagcag ataggccgcg ccggtggccg tcccgaagaa
     3781 cagcgccgtg aagaccacga atccgaccgt ccccccggcg ctgatccgcc ccaccacttg
     3841 gtccgcctcg gtgatccgcc cctgggcccc atggccggcg gtcaccgcca gcagccgcat
     3901 caccagccgc ccgccggtcc cagcggccat gaccccggcg ccgatcccgg cgatgacggc
     3961 caggttcacc gcccacaggt accgccgcac gaccagtccc accggcgggg ggtcgcccgg
     4021 cgcggtcgcg ggcggtgact cgaccgccag gccaccccag cgaaccaccg cgaccagccc
     4081 ggccagcacc actaggacgc aggcaccgat caccaccatg cccgcagtat gacggcttga
     4141 cttgctcagc tcgaacggaa acgcagacgg cgcctcggtg ggaggcgccg tctggacgaa
     4201 cc
//
LOCUS       DATADN010000117         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_918329, whole genome shotgun sequence.
ACCESSION   DATADN010000117 DATADN010000000
VERSION     DATADN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918329"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(815..>2097)
                     /locus_tag="VHH53_01550"
     CDS             complement(815..>2097)
                     /locus_tag="VHH53_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX2288897.1"
                     /translation="GDPPSVLFGKLYARSHLRSDRWYKLGRELLYGRLEDEKAFNTVG
                     RLVQQEDYALRLLRDAGLPTPAPYGVVELTPEREYLLVTEFFAGATELGEAEVDEQVI
                     DDGVGIIRKLWDAGLAHRDIKPANLLVRDGRMLLIDVAFVQAHPSPWRQAVDLANMML
                     CLALRSDPGLVYERALRQFSVEEITEGFAATRGLTMPSQLRNLMRAQGRNLHAEFLDL
                     LPERPRPISIQRWSMRRVGLAVLTLLFAAVVLNVAGSVLISDGGVETAPIRAQALRCE
                     PHEPLLLMAQSVPTASLVPCIEILPVGWTLDGVVVGNDRSRFTMTSDRGGVLVTELAA
                     SCDLAGAVELTSERPGARRYVRIERNVTGLAMTRAYTFKGGCVTQRLVAPEASQQQLA
                     SEALFAMGFTTRDALAAALRRDSGGRLDLDMTAR"
BASE COUNT          346 a          704 c          732 g          315 t
ORIGIN      
        1 cccatcccaa gcgacggctt gtacgcgtgg cgaactccgg agaatgacaa cgggtcgccg
       61 gggtccctcg ggggcgacag tcggtggctt cctctctgcc ggtgaccgaa cccttgagct
      121 tgccgccgcc tgcgacgtac ggcccgcaac ctcgaggcaa gcgatcggtc ccctaccgcc
      181 atcgacatcc ggtcctcagg gcaggcggaa ggccaggaca ggcctaggcg aacgagcgct
      241 ggtcaccaca agcccactga cccgggcagc cgttcccggc gcacgtcgaa gagaacccga
      301 acggctatgc agtgaaggga tctcatgccg aacgatcggc gattcacacg attcggacac
      361 aagcctgatg agctcaccgc ccgcgtgaac cgaccccgaa cgcgaagatg accagtgttt
      421 gcgctggtca tcggcttggt ggagcccccc gccggaatcg aaccggcgac cccatcctta
      481 ccatcgatgc gcgggtggtt cacgacgcca tgcagcacct cacgtcccca cacaaccgcg
      541 caggtgaacg gcgctgccga gggttgggtg gtgaggctac gtgaggctgc gcgtagcacg
      601 gtttctggca aatctctggc aaggccccgt gcgtagccgt ctattggatc aatgtcgacg
      661 ctatcgcccg gcagtcctgg actacaagcg gctgcacctg cggtgcagtg ctctcctcag
      721 acgtttctag gtcgatgatc gcgacctgga cgatccggcg ggtaggggcg gcctcggcac
      781 ggttggcacg tgctcggtca tcggaacgat ctcctcatct ggcggtcatg tcgaggtcga
      841 gccgaccgcc ggagtcgcgc cgaagggcgg cggccagggc gtcgcgggtg gtgaagccca
      901 ttgcgaacag tgcctcgctg gccagctgtt gctggctggc ctcgggtgcg accaggcgct
      961 gggtgacgca gccgcccttg aaggtgtagg cgcgggtcat cgcgagcccg gtgacgttac
     1021 gctcgatccg cacgtagcgt cgtgcccccg gccgctcgga ggtcagctcg accgccccag
     1081 ccaggtcaca ggaagcggcg agctctgtga ccaggacccc gccgcggtcg ctggtcatcg
     1141 tgaaccggga ccggtcgttg ccgaccacca cgccgtccag cgtccagccg accggcagga
     1201 tctcgatgca cggcaccaat gaggcggtgg gcaccgactg ggccatgagc aggagcggct
     1261 cgtggggctc gcagcggagc gcctgcgccc ggatgggcgc ggtttccacc cccccgtcgc
     1321 tgatgagcac gctcccggcg acattgagga cgacagcggc gaagagcagg gtgagcacgg
     1381 ccagcccgac ccgccgcatg ctccagcgct ggatggagat cgggcggggc cgctcgggca
     1441 gcaggtcgag gaactccgcg tggaggttac gcccctgggc ccgcatgagg ttgcggagct
     1501 gggacggcat ggtcagtccg cgggtggcgg cgaagccctc ggtgatctcc tcgaccgaga
     1561 actggcgcag ggcccgctcg tagaccagcc cggggtcgct gcgcagggcc aggcagagca
     1621 tcatgttggc cagatcgacg gcctggcgcc agggactcgg gtgggcctgg acgaaggcca
     1681 cgtcgatcag caacatgcgc ccgtcgcgga ccagcaggtt ggccggcttg atgtcgcggt
     1741 gggccaggcc ggcgtcccac agcttgcgga tgatgccgac cccgtcgtcg atgacctgct
     1801 cgtccacctc ggcctcgccg agctcggtgg cgccggcgaa gaactcggtg accagcagat
     1861 actcccgttc cggggtgagc tctacaaccc cgtagggcgc cggcgtgggc aggccggcgt
     1921 cgcgcagcaa ccgcagggcg tagtcctcct gctggaccag ccggccgacg gtgttgaacg
     1981 ccttctcgtc ctccaggcgg ccgtacagca gctcccggcc gagcttgtac caacggtccg
     2041 agcgcaggtg gctgcgggca tacagcttgc cgaacaagac cgagggcggg tcgccct
//
LOCUS       DATADN010000118         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1069066, whole genome shotgun sequence.
ACCESSION   DATADN010000118 DATADN010000000
VERSION     DATADN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069066"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..>1758
                     /locus_tag="VHH53_01555"
     CDS             112..>1758
                     /locus_tag="VHH53_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2288898.1"
                     /translation="MRTFWRAALGAVVALVLAGCGGPSSGGGTAGGGVAGGIHNPSDA
                     KGGTLWFANSGDWDSLDPADTYYAYSFNFIHNYGRALVMFKPAPGQQGATLVPDLAES
                     LGRSSDDSKTWTYTLRQGVKFEDGTPVTSKDIKYAVERSLDKTTFPNGPTYFNDVLDL
                     QGYTSPYSDPDPDKLGLKAIETPDDRTIVFRLRKPFSGFDYLAQLPATIPVPQALDTG
                     SKYKEHVVSTGPYMFETNELGKRFTMVRNPHWDPATDPNRRPLPDRIAVDLNVNADDV
                     DNRLISGDLDVSIEGSGVGPSTQGRILADQDLRANTDSALVARLWYTALNSDVAPLDN
                     IHCRKAVLYATDRTGYQRAYGGATGGEIATNLLPPVIPGAERFNLYPSPNNEGDIAKA
                     RDELAQCGQPNGFTTAISYRAERPKEKATAEALQQSLARAGINLEIKPYPLTDYGRLY
                     AGKPDYAKANGLGLMVFGWAPDWPDGYGFLSQIVDSRVIRTTGGNTNLGIKDPAIDQL
                     LDHAIMTTDTAARERIWVDIDRKVMENAFVLPGVWAKGLLY"
BASE COUNT          334 a          613 c          542 g          269 t
ORIGIN      
        1 atcacgtgct ggcgttcaac ctgttcggcg atggcttgcg ggacgcgctg actccccggt
       61 cgtgatcaag gcccgcccga agaaggcaaa ttgacaaagg ggtgacacga gatgagaact
      121 ttttggcggg ctgcgctcgg cgcggtcgtc gcgctggtgc tggccgggtg cggcgggccg
      181 agctccggtg gcggcaccgc cggtggcggc gtggccgggg gaatccataa tccctcggac
      241 gccaagggcg gcaccctgtg gttcgcgaac tccggtgact gggactccct tgacccggcc
      301 gacacctact acgcctactc cttcaacttc atccacaact acgggcgagc tctagtgatg
      361 ttcaagccag caccgggcca gcagggcgcc acgctggtgc ccgaccttgc cgagtcgctg
      421 ggccgctcca gcgacgactc gaagacctgg acctacaccc tgcgccaggg ggtgaagttc
      481 gaggacggca cccccgtcac cagcaaggac atcaagtacg ccgtcgagag gtcactggac
      541 aagacaacct tccccaacgg cccgacgtac ttcaacgacg tcctcgatct acagggctac
      601 accagcccgt actccgatcc cgacccggac aagctcggcc tgaaggcgat cgagaccccg
      661 gatgaccgga cgatcgtctt tcgcctgcgc aagccgttca gcggtttcga ctacctcgcc
      721 cagctgccgg ccaccattcc ggttccgcag gcgttggata ccgggtcgaa gtacaaggag
      781 cacgtcgtct cgactggccc gtacatgttc gagaccaacg agctgggcaa gcgcttcacg
      841 atggtccgca acccgcactg ggacccggcc accgacccga accgccgccc gctgcccgac
      901 cgcatcgcgg tcgatttgaa cgtcaacgcc gacgatgtcg acaaccggct catctccggt
      961 gatcttgacg tctcgatcga gggctccggg gtcgggccgt cgactcaggg ccggatcctc
     1021 gccgaccagg acctgcgcgc gaacaccgac tccgcgctgg tcgcccggct gtggtacacc
     1081 gcgctcaatt ccgatgtcgc accgctggac aacatccact gccgcaaggc ggtgctgtat
     1141 gccaccgacc gcaccggtta ccagcgcgcc tacggtggag cgaccggcgg cgagatcgcc
     1201 acgaacctgc tgccgccggt gatcccgggc gcggagagat tcaacctcta cccgtcgccg
     1261 aacaacgagg gtgacatcgc caaggcccgc gacgagcttg cccagtgcgg ccagcccaac
     1321 ggcttcacca ctgccatctc ctaccgcgct gaacggccca aggagaaggc caccgccgaa
     1381 gcactgcagc agtcactggc gcgggccggg atcaacctgg aaatcaagcc gtacccgctg
     1441 accgactacg ggcgcctgta cgccggcaag ccggattacg cgaaggccaa tggtctgggc
     1501 ctgatggtct ttggctgggc cccggactgg cctgatggct acgggttcct gtcccagatc
     1561 gtcgacagcc gggtcatccg gaccaccgga ggcaacacca acctgggaat caaggacccg
     1621 gcgatcgacc agttgctcga ccacgcaata atgaccaccg acaccgcggc gcgggaacgg
     1681 atctgggtcg acatcgaccg caaggtgatg gaaaacgcgt tcgtcctacc cggcgtctgg
     1741 gccaagggcc tgctgtac
//
LOCUS       DATADN010000119         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1370291, whole genome shotgun sequence.
ACCESSION   DATADN010000119 DATADN010000000
VERSION     DATADN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370291"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /gene="leuA"
                     /locus_tag="VHH53_01560"
     CDS             complement(<1..1514)
                     /gene="leuA"
                     /locus_tag="VHH53_01560"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX2288899.1"
                     /translation="MHAPTAVAPLDQARWNLQRGSAMPAHRYRPFAQTVEAVSLPDRT
                     WPDITISRAPQWCAVDLRDGNQALIDPMSPARKRRMFELLVRMGYKEIEVGFPSASQT
                     DFDFVREIIIEGAVPEDVRIQVLSQCRPELIERTFEALEGAHKAIVHIYNSTSILQRR
                     VVFREEREGIKKIATSAAEMLVEYAGKYSDTDFRFQYSPESYTGTELHYAAEVCNAVT
                     EIWQPTADAPVIVNLPATVEMATPNVYADSIEWMHRNLERRSAVVLSLHPHNDRGTAV
                     AAAELGYQAGAQRIEGCLFGNGERTGNVCLVTLAMNLFSQGIDPQIDFSDIDEIRRTV
                     EYCNQLPVSERHPYGGDLVYTAFSGSHQDAINKGLRAMQADAARAGIRVDEHRWEVPY
                     LPIDPKDVGRTYEAVIRVNSQSGKGGVAYVMKAEHQLELPRRLQIEFSRVIQQHTDTG
                     GGEVSPQQIRDLFAEHYLDRNTPLRVLRHQLSDDGEGHDEIQANVLVEGEEHQIT"
BASE COUNT          297 a          583 c          612 g          371 t
ORIGIN      
        1 gtgatctggt gttcctcacc ttccaccagc acgttggcct ggatctcgtc gtggccctcg
       61 ccgtcgtctg aaagctggtg ccgcagaacc cgcagcgggg tgttccggtc taggtagtgc
      121 tcggcgaaga ggtctcgaat ctgctgcggc gagacctcgc cacccccagt gtcggtgtgc
      181 tgttggatga cgcgagagaa ctcgatctgc agccgtcgcg gcagctccag ctggtgctcg
      241 gccttcatca cgtaggcgac gccgcccttg ccggactgag agttgacccg gatcactgcc
      301 tcataggtgc gcccgacgtc tttggggtcg ataggcaggt acggcacctc ccagcgatgc
      361 tcatccaccc tgatgccggc tcgagcggcg tcggcctgca tcgctcgcaa tcccttgttg
      421 atcgcgtcct ggtggctgcc ggaaaacgcc gtgtaaacca agtcgccgcc gtagggatgc
      481 cgctcggaaa ccggcagctg gttgcagtac tcgaccgtgc ggcgtatctc gtcgatgtcg
      541 gagaaatcga tttgggggtc gattccctga ctgaacaggt tcattgccag cgtcaccagg
      601 cacacgttgc cggtgcgttc cccgttgccg aacaggcagc cttcgatacg ttgcgcgcct
      661 gcctgatagc ccagctcggc ggcggcgacc gcggtgccac ggtcattgtg gggatgcagc
      721 gagaggacca ccgcactgcg ccgttccagg ttgcgatgca tccactcgat ggagtcggcg
      781 tagacgttgg gcgtggccat ctcgacggtg gccggcaggt tcacgattac cggtgcgtcc
      841 gcggtgggtt gccagatttc ggtgaccgcg ttgcacactt cggcggcgta gtgcagttca
      901 gtaccggtgt aggactccgg cgagtactga aagcggaagt cggtgtcgga gtacttgccc
      961 gcgtactcca caagcatctc ggccgctgag gtggctatct tcttgatccc ctcgcgctcc
     1021 tcccggaaca ccacccggcg ctgcaggatc gacgtcgagt tgtagatgtg cacgatggcc
     1081 ttgtgcgcgc cttccagcgc ctcgaaggtg cgctcgatca gttccggccg gcactgtgac
     1141 agcacctgga tgcgcacatc ctcgggcact gcaccctcaa tgatgatctc tcggacgaag
     1201 tcgaaatcgg tttggctggc cgaagggaac ccaacttcga tctccttgta tcccatccgg
     1261 accagcagct cgaacatgcg ccgcttgcgt gccgggctca tcgggtcgat caaggcctgg
     1321 ttgccgtccc ggagatcaac cgcacaccac tgcggtgccc ggctgatcgt gatgtcaggc
     1381 caggtgcggt ccggcagcga tacggcttcc acagtctggg cgaagggtcg gtagcgatgc
     1441 gcgggcattg cgctgccgcg ctggaggttc caacgggcct ggtcgagtgg agctacggcg
     1501 gtgggagcgt ggatgcggct gctagtgcct gcggtgaacg tactcgattc ggactcgcta
     1561 gggtcgatat cagccgcttt gggagtcctc gtctgcttcg gtgccatctc tgcgctttct
     1621 gatagccgga caatccgacc ggcggatgcg cgcgggcgac tccgcgacgg ggggccggtc
     1681 gtcgtcagac cccctcgcgg cggcgaagca gcagggcgcg tgccacgcga tgacggtagc
     1741 cgggtcctgc cagcaacacg caaccaccgt cccagattgt gagatccccg gtgtgcccgt
     1801 cccctcacga acagtgcacg ccgtggcaga ccgtggcaga ctttcggtat gggtggaact
     1861 gtt
//
LOCUS       DATADN010000120         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_466332, whole genome shotgun sequence.
ACCESSION   DATADN010000120 DATADN010000000
VERSION     DATADN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466332"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="tal"
                     /locus_tag="VHH53_01565"
     CDS             complement(<1..1000)
                     /gene="tal"
                     /locus_tag="VHH53_01565"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX2288900.1"
                     /translation="MNPLVRLAELGQSVWYDYITRDLLVSGELDRLIREDGLRGMTSN
                     PTIFEKAIAGSSLYDDDIRRLREQGSGAADIFEAIAVADVQAACDAFASLYKESGKRD
                     GFVSLEVNPRLAQDAEATLQEARRLWKAVGRPNAMIKIPGTQAGLTAVARAIASGINV
                     NVTLLFSVERYSEVIEAFVQGLESRLQQRQPIDSIASVASFFVSRVDGRIDPLLDRNG
                     AGGLRGKIAIANAALAYRLFRQSLSSERWARLAAEGAQPQRPLWASTSTKDPKYPDVY
                     YVEALIAPDTVNTLPPETLTAYRDHGRPAVRIDEAMAAAAAQLSGLRDADIDLPAIT"
     gene            complement(1030..>1952)
                     /locus_tag="VHH53_01570"
     CDS             complement(1030..>1952)
                     /locus_tag="VHH53_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2288901.1"
                     /translation="AAAVPNLIGGSADLGGSTGTTLKQGGTFGPGTSGRTFHWGVREH
                     GMAACHNGIAAHGGLRAFGSTFLVFSDYMKPAIRLAAIMRLPVIYIGTHDSIGLGEDG
                     PTHQPVEHLAMLRAIPNLVTLRPADPGETVEAWKVAMERRDGPTMLVLTRQKVPLLER
                     AGVDAAEAVRRGAYVVQREGGKAPDAIIIATGSEVPVAVAAARQLESEGVPTRVVSMP
                     SWELFAAQPAGYRDSILPPSVRTRVAVEAAGPMGWSRWTTDEGAMVGMEGFGASAPGE
                     RLFQEFKLTPEHVAATVRLLRSRGGTQSGE"
BASE COUNT          307 a          683 c          641 g          321 t
ORIGIN      
        1 gggtgatggc cgggagatcg atgtcggcat cgcgcagccc gctgagctgc gccgcggcag
       61 ccgccatggc ctcgtcgatc cgaaccgccg gccggccgtg atcgcggtac gcggtgaggg
      121 tctccggcgg aagggtgttg accgtgtcag gcgcgatcag cgcctccacg tagtagacgt
      181 cgggatactt cgggtccttg gtgctggtgg acgcccagag cggacgctgg ggctgggcgc
      241 cctccgccgc gagccgggcc cagcgttccg acgacaacga ctgccggaac agccggtacg
      301 ccagcgccgc attcgcgatg gcgatcttgc cccgcagtcc gcccgctccg ttccggtcga
      361 gcagcggatc tatcctgccg tccacccggc tcacgaagaa gctcgccacc gacgcgatcg
      421 aatcgatcgg ctgccgttgc tgcaaccgcg actcgagtcc ctgaacgaag gcctcgatca
      481 cctccgagta gcgctccacc gaaaagagca gggtgacgtt gacgttgatc cccgacgcga
      541 tggcccgcgc gacggcggta aggccggcct gcgttcccgg gatcttgatc atggcattgg
      601 gccggcccac tgccttccag agccgcctcg cctcctgcag cgttgcctcg gcatcctgag
      661 ccagccgggg gttcacctcg agcgacacga atccgtcgcg cttccccgat tccttataga
      721 gcgatgcgaa ggcatcacac gctgcctgca cgtcggcgac tgcgatggcc tcgaaaatgt
      781 cggcggcgcc gctgccctgc tccctcagcc ttcggatgtc gtcatcgtat agcgagcttc
      841 ccgcgatggc cttctcgaag atggtgggat tcgaggtcat gccccggagt ccatcttccc
      901 ggatcagccg gtccagctcg cccgagacga gcaggtcgcg ggtgatgtag tcgtaccaga
      961 cgctctggcc cagctcggcg agccggacca ggggattcat cgccggtgtt gtctcccgat
     1021 tcatctagct cactctccgc tttgcgtacc accgcgcgag cggagcagcc gcacagtggc
     1081 ggccacatgc tcgggtgtca gcttgaattc ctgaaacagg cgctcgcccg gagccgaagc
     1141 cccgaatcct tccatcccga ccatggcacc ttcatcggtg gtccagcggg accagcccat
     1201 cgggccggcg gcctcgacgg ccacccgagt ccggaccgag ggcggcagga tcgagtcgcg
     1261 gtagcccgcc ggctgcgccg cgaacagctc ccacgagggc atcgaaacca cccgggtggg
     1321 gacgccttca ctctccagct ggcgggcagc ggccaccgct acggggactt ccgatccggt
     1381 cgcgatgatg atcgcgtcag gagccttccc tccctcgcgt tgcaccacat aggcgccccg
     1441 gcgcacggct tcggccgcgt ctaccccggc gcgctcgagc agcggaactt tctgccgagt
     1501 gagcaccagc atggtggggc cgtcgcggcg ctccatggcc accttccagg cctcgaccgt
     1561 ctcaccggga tcggccgggc ggagggtgac caggttggga atggcgcgaa gcatcgcaag
     1621 atgctccacc ggctggtggg tggggccgtc ctcgcccagg ccgatcgaat cgtgggtccc
     1681 gatgtagatc accggcagcc gcatgattgc ggcgagccgg atggcgggct tcatataatc
     1741 ggaaaacacc aggaacgtgc tgccgaacgc ccggagtcca ccgtgggcgg caatgccgtt
     1801 gtgacacgct gccatcccgt gctcccgcac tccccagtgg aaggtgcgcc ccgaggtgcc
     1861 cggtccgaag gttccgcctt gcttgagggt ggtgcctgtg ctccctccca gatcggcgga
     1921 gccgccgatc aagttgggca ccgccgcggc aa
//
LOCUS       DATADN010000121         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_767833, whole genome shotgun sequence.
ACCESSION   DATADN010000121 DATADN010000000
VERSION     DATADN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767833"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHH53_01575"
     CDS             <1..331
                     /locus_tag="VHH53_01575"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEX2288902.1"
                     /translation="VNWFRRGADQRFLWPGFGENSRVLKWIIERIDGRAAAADTPIGR
                     VPSPGQLDLSGLDVPMGDVTAALAVDVDEWLAELPSIEQWFGKIGDALPSSMRDELDA
                     LKWRLVS"
     gene            358..570
                     /locus_tag="VHH53_01580"
     CDS             358..570
                     /locus_tag="VHH53_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX2288903.1"
                     /translation="MSSPRPRSLRWSNWNLLLLVPLLILVTPVYNRIDPRLLGFPFFY
                     WYQFLWVPVGVICTAVVYLKTRDDRR"
     gene            567..>1895
                     /locus_tag="VHH53_01585"
     CDS             567..>1895
                     /locus_tag="VHH53_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX2288904.1"
                     /translation="MNPQWTEIIVFTLLFGFVTVLGFVAVRWRAAATLEHLDEWGLGG
                     RKFGSWITWFLIGGDLYTAYTFVAVPALIFGAGALGMFALPYTIIAYPLVFMPLLRLW
                     SVSRVHGYVTPADFVHGRYGSSGLALVIAITGMVATMPYIALQLVGLEAVVRTMGLNG
                     TGIVGHLPLIVAFVILAVYTYKSGLRAPALIAFVKDGLIYLVIAVAVVYLPAKLGGWG
                     TIFDAANTKFTNSPAPADGVLLNANNQLQFATLAFGSALALFLYPHSVTGVLASGGRN
                     VIKRNMSALPAYSLLLGLIALLGYAAIAAGTTPIVNAATGRPDSNTIIPVLFGTQFPA
                     WFAGIAFAAIGIGALVPAAIMSIAAANLFTRNIYKEYLKPDATPQQEANVSKLTSLVV
                     KGGALLVVLALSPQFSIDLQLIGGVIILQTLPAILLGLWKLPVHRFALFAG"
BASE COUNT          261 a          621 c          629 g          384 t
ORIGIN      
        1 cgtgaactgg tttcgccgtg gtgccgacca gcgcttcctg tggccaggct tcggggagaa
       61 cagccgggtg ttgaagtgga tcatcgagcg gatcgatggc cgggctgctg cagctgatac
      121 cccgatcggc cgggttccca gccctggtca gctcgacctg tccgggctgg acgtcccgat
      181 gggcgacgtg actgctgcgc tggcggtgga cgtcgatgag tggctggccg agctcccgag
      241 catcgagcag tggttcggca agatcggtga tgcgctgccc agctcgatgc gtgacgagct
      301 cgacgccctc aagtggcggc tagtctcctg aggcctcccg ccccgcataa tgtcgagatg
      361 tcgtcgcccc gaccgcggtc gttgcgctgg agcaactgga atctgttgct gctggtgccg
      421 ctgctcatcc tggtcacacc ggtgtacaac cgcatcgatc cgcggctgct gggtttcccg
      481 ttcttctact ggtaccagtt cctgtgggtg ccggtcgggg tgatctgcac cgctgtggtg
      541 tacctgaaga cgcgggatga ccggcgatga acccgcagtg gaccgagatc atcgtcttca
      601 cgctgctgtt cggttttgtc acggtgctgg gcttcgtcgc ggtgcggtgg cgggccgcgg
      661 ccacgctgga gcacctggac gaatgggggc tcggcggccg gaaattcggc tcctggatca
      721 cctggtttct catcggtggt gatctctata ccgcgtacac gttcgtcgcg gtcccggcgt
      781 tgatcttcgg agccggcgcg ctgggcatgt tcgcgctgcc ctacacgatc attgcctacc
      841 cgctggtgtt catgccgctg ctgcggttgt ggtcggtgtc ccgcgtgcac ggttacgtca
      901 cgcccgccga tttcgtgcac ggacgttacg gctcgtcagg gctcgcgctg gtgatcgcca
      961 tcaccgggat ggtggcgacc atgccctaca tcgcattgca gctggtcggg ctggaggcgg
     1021 tggtacgcac gatggggctc aacggcaccg gaatcgtcgg ccacctgccg ctgattgtgg
     1081 cgtttgtcat cctcgccgtc tacacctaca agtccgggtt gcgggccccg gctctgatcg
     1141 ccttcgtcaa ggacgggctg atctacctgg tgatcgcggt ggcggtggtg tacctgccgg
     1201 ccaagctggg cggctggggc acgatcttcg acgccgcgaa caccaagttc accaactccc
     1261 cggcgcccgc cgatggggtg ctgctcaacg ccaacaacca gttgcagttc gcgacgctgg
     1321 ctttcggctc ggcactggca ctgttcctgt acccgcactc ggtgaccggg gtgctggcct
     1381 ccggcggccg caacgtgatc aaacggaaca tgtcggcgct gccagcgtat tcgctgctgc
     1441 tcgggctgat cgcgctgctc ggatacgcgg ccatcgccgc ggggaccacg cccatcgtca
     1501 acgccgccac cggccggccg gactccaaca ccatcatccc ggtgctcttc ggtacccagt
     1561 tcccggcctg gttcgccggc atagcctttg ccgccatcgg gatcggcgcc ctagtgccgg
     1621 cggcgatcat gtcgatcgcg gccgcgaacc tcttcacgcg caacatctac aaggagtacc
     1681 tcaagcccga cgcgacgccg cagcaggagg cgaacgtctc gaagctgacg tcgctcgtcg
     1741 tcaagggcgg cgcgctgctc gtcgtgctgg cgctgagccc gcagttctcg atcgacctgc
     1801 agctcatcgg cggggtgatc atcctgcaga cgctgccggc gatcctgctc ggcctctgga
     1861 agctgccggt gcaccgcttc gcgctgttcg ccggt
//
LOCUS       DATADN010000122         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_617556, whole genome shotgun sequence.
ACCESSION   DATADN010000122 DATADN010000000
VERSION     DATADN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617556"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..686
                     /locus_tag="VHH53_01590"
     CDS             93..686
                     /locus_tag="VHH53_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX2288905.1"
                     /translation="MQTTAAPSMLRHLWIYTVVSGVLAVLLGVLIFVRPGAAILVTAI
                     FFGAYLLVTGVAQVVLAFSVRSSFGGRALLFISGAAALVLAVLCFINLSNSIDLLAIW
                     IGVGFIFRGVATAMSAFSDQSLPGRVWEIIVGVISVIAGIIMFVAPLEGLVALTQVTG
                     IILVVIGIVEVVAGIRIRRESAAITGTATSPQADNVT"
     gene            781..>1854
                     /locus_tag="VHH53_01595"
     CDS             781..>1854
                     /locus_tag="VHH53_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2288906.1"
                     /translation="MDALDVSRWQFGITTVYHFIFVPLTIGLAPLVAVMQTMWVVTDN
                     PVWYRLTRFFGKLFLINFAIGVATGIVQEFQFGMNWSEYSRFVGDVFGAPLAMEGLAA
                     FFFESTFIGLWIFGWTRLPRLVHLACIWIVAIAVNMSAFFIIAANSFMQHPVGAHFNP
                     ETGRAELTSIVALFTNNTAIAAFSHAVAGSFLTAGTFVACVCAWWMVRSHRDGQTPAE
                     KHTAAALFRPATILGCLVAVVSAAALFYTGDVQGKLMFEQQPMKMASAESLCHSATDP
                     AFSVLTVGTHNNCDSVTHLIEVPYVLPFLAEGEFSGVHLEGVKDLQEQYEQKFGPGDY
                     RPNLFVTYWSFRAMIGLLAVPVLF"
BASE COUNT          284 a          641 c          550 g          379 t
ORIGIN      
        1 tccggtaacg gaacattaac gacccggcac cgacatctca gccgtactgc caaatcggca
       61 ctacgcaccg agtgctgtgc cagactgcgc gcatgcaaac taccgctgcc ccaagcatgt
      121 tgcggcatct gtggatctac accgtggtct caggcgtgct ggcggtgctc ctcggcgtcc
      181 tgatcttcgt ccgtccgggc gcggccatcc tggtgacggc catcttcttc ggcgcctacc
      241 tactcgtgac cggtgtcgca caggtcgtgc tggccttcag cgtccggtcg tcgttcggcg
      301 gccgcgcgct gctgttcatc agcggtgccg ccgccctggt gctcgccgtg ctgtgtttca
      361 tcaacctttc caactcgatc gacctactgg cgatctggat cggtgtcggc ttcatcttcc
      421 gcggtgtcgc gaccgcgatg tcagcgttca gcgaccagtc actgccgggt cgcgtctggg
      481 agatcatcgt cggtgtcatc agcgtgatcg ccggcatcat catgttcgtg gcgccgttgg
      541 aggggctggt cgccctcacc caggtgaccg gcatcatcct cgtcgtcatc ggcatcgtcg
      601 aggtcgtcgc gggcatccgg atccgccgcg agtcggcggc catcaccggc acggccacct
      661 cgccacaagc ggacaacgtg acgtaaccca ccgaggaccg accaaagtcc ctgctccaac
      721 ccccgctttc gctactacac tctgtcgtag ataggtgtag agcccgcgag agttggtcag
      781 atggacgctt tggacgtgtc acggtggcag ttcggcatca ccaccgtcta ccacttcatc
      841 ttcgttccgc tgaccatcgg cctcgcgccg ctggtcgcgg tcatgcagac catgtgggtc
      901 gtgacggaca accccgtctg gtaccgcctc acccggttct tcgggaagct gttcctgatc
      961 aacttcgcga tcggtgtcgc caccggcatc gtccaggaat tccagttcgg gatgaactgg
     1021 agcgaatact cccgcttcgt cggcgacgtc ttcggggcgc cgctggcgat ggaggggctc
     1081 gccgccttct tcttcgagtc caccttcatc ggcctgtgga tcttcggctg gacccgcctg
     1141 ccccgcctgg tgcacctggc gtgcatctgg atcgtcgcta tcgcggtcaa catgtcggcg
     1201 ttcttcatca tcgccgcgaa ctcgttcatg cagcaccccg tgggggcgca cttcaacccg
     1261 gagaccggac gcgccgaact gaccagcatc gtcgcgctct tcaccaacaa caccgcgatc
     1321 gccgcgttca gccatgcggt ggccggatcg ttcctgacag cgggcacctt cgtcgcgtgc
     1381 gtctgcgcct ggtggatggt gcgctcccac cgcgacgggc agacgccggc cgagaagcac
     1441 acggccgccg cgctcttccg gccggccacc atcctcggct gcctggtcgc cgtcgtctcc
     1501 gccgccgcgc tgttctacac cggtgacgtc cagggcaagc tgatgttcga gcagcagccc
     1561 atgaagatgg catccgcgga gtcgttgtgc cacagcgcca ccgaccccgc attctcggtg
     1621 ctgacggtcg gcacgcacaa caactgtgac agtgtcacgc acctcatcga ggtgccctac
     1681 gtgctgccgt tcctggccga aggcgaattc agcggcgtcc acctcgaagg cgtcaaagat
     1741 ctgcaggagc agtacgagca gaagttcggg cccggcgact accggcccaa cctgttcgtc
     1801 acgtactggt ccttcagggc gatgatcggt ctgcttgctg ttccagtgct gttc
//
LOCUS       DATADN010000123         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_315871, whole genome shotgun sequence.
ACCESSION   DATADN010000123 DATADN010000000
VERSION     DATADN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315871"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VHH53_01600"
     CDS             <1..603
                     /locus_tag="VHH53_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288907.1"
                     /translation="QQLNQQHPHRAYQLAPVSGVLGGLDMVIHYPPARLRQNSDRPTA
                     ITNVLVATSGSSSRTGDIALGAPTCSARTRQGRWALYPNGDQVVVVWPGGALAGPPVE
                     VAHELNGLGGDHHDGLDHAAAQAIRQFLTDPAATGAARWSSTRARTWSSPAVGYWLSG
                     LTNQPGHHTHTPARQPGAPTPAAPIRLPGPSPEPAPARAA"
     gene            complement(858..1769)
                     /locus_tag="VHH53_01605"
     CDS             complement(858..1769)
                     /locus_tag="VHH53_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2288908.1"
                     /translation="MQRVQRLLRLVDRVQQRSRWLGFTLAVVRKFADDNGAALTTVVA
                     YNAFFALFPLLLVVVTVLGFLLGRDSGFQQRLLGSAVAEFPIIGDQVRDNIHGLRGSG
                     VGLMVGLMVFAWGARGLTQVTQQAMAEIWNIPGRQRPRFWARQVRGLLLLVVFAVGLA
                     ATSLLTWLGSYGGKAVAVALANLAAAAAVNVGLFLLVFRVLTPKQIPARQLVAGALVA
                     GVAWQVLQAVGGFLVGHYLRHTSQVYGVFAIVLGLLFWLYLGAQLTLYAAEVNVVAAR
                     RLWPRSLLQPPPANTNPPASAVDLANQ"
     gene            1895..>2107
                     /locus_tag="VHH53_01610"
     CDS             1895..>2107
                     /locus_tag="VHH53_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288909.1"
                     /translation="MSAERPAAQEPDQAPADSPRRAEPEMAAPADAEPTLSQHTIEPT
                     RTSMVWTMVGIGVILLVAILVFILQNG"
BASE COUNT          371 a          781 c          680 g          275 t
ORIGIN      
        1 cagcagctca atcagcagca ccctcaccgc gcttaccagc tagccccggt cagcggcgtc
       61 cttggaggac tcgacatggt gatccactac ccgcccgcgc gtcttcgcca gaacagcgac
      121 cggccaaccg cgatcaccaa cgtccttgtc gccacctcgg ggtcgtcctc gcggaccggt
      181 gacatcgcgc ttggcgcccc gacctgctcg gcccggaccc gccagggccg ctgggcgctg
      241 taccccaacg gcgaccaggt cgtggtcgtc tggccgggcg gcgcgttggc cggaccaccg
      301 gtcgaggtcg cccacgagct caacggtctg ggcggtgatc accacgacgg tctcgaccac
      361 gcggccgccc aggcgatccg gcagttcctg accgaccccg ccgccaccgg ggccgcccgc
      421 tggtcgtcga cgcgggcgcg tacctggtcc agcccggcgg tgggctactg gctcagcggc
      481 ctcaccaacc agcccggcca ccacacccac acaccagcca gacagcccgg cgctccaaca
      541 cctgctgcac cgatccggct gcccggcccc agcccagagc cggccccagc acgagccgcc
      601 tgacgcacaa ccatctacga tcccacgacc gagacccacc gccgcccgat tcgttccggc
      661 gctcagcaag cagctggtgc atcgtcgcca gcgacatcgc ggtgttggcc gggggttggc
      721 ggttggggcg tcgcaacgtc ttgctggtcg tcgagcaacc ggtaaggccg tcgctgccgg
      781 gtcggccgat ggctgtgccg gtcggcggtg acgtcgacct ggcggcttgc gcaggttgac
      841 gttcctagtt gacgttccta ttggttggcg aggtcaacgg ctgaggcggg tgggttggtg
      901 ttggccggcg gtggctgcag caggctacgt ggccagagcc gtcgggcggc gacgacgttg
      961 acttcggcgg cgtacagggt gagctgcgcg cccagataca accagaacag cagaccaagc
     1021 acgatcgcaa acaccccata cacctggctg gtatggcgca ggtagtggcc gaccaggaag
     1081 ccaccgacag cctgcaggac ctgccacgcc acccccgcga ccagcgcacc ggcaaccagc
     1141 tgccgggccg ggatctgttt tggggtcagc acccggaaca ccagcaggaa cagcccgacg
     1201 ttgacggctg ccgcggccgc gaggttggcg agcgccaccg cgaccgcctt cccaccgtag
     1261 ctgccaagcc aggtcagcag gctggtcgcg gccaggccaa ctgcgaagac caccagcagc
     1321 agcagcccac ggacctgccg ggcccagaag cggggccgct gcctgcccgg gatgttccag
     1381 atctcggcca tcgcctgctg ggtcacctgg gtcagcccgc gggcacccca cgcgaagacc
     1441 atcaagccga ccatcaggcc gacgccgctg ccgcgcagcc cgtggatgtt gtctcgcacc
     1501 tggtccccga tgatcgggaa ctcggccacc gccgagccca gcaggcgctg ctggaagccg
     1561 gagtcgcggc cgagcaggaa cccgagcacg gtgaccacga ccagcagcag gggaaacaac
     1621 gcgaagaacg cgttgtaggc gaccaccgtg gtcagcgcgg cgccgttgtc gtcagcgaac
     1681 ttccgcacga ccgccaacgt gaagcccagc caccgggaac gctgttgcac gcggtcaacc
     1741 aaccgcagca gccgttggac acgctgcatg ccagcacatc acccccgcac gccagggcca
     1801 gttgcctgct gtcctgaccg caagcttgtg gtctgattgg cgcgattacc agtcgagcgg
     1861 gcaaccatcc tccggaacag cagcgaggac gaccatgagc gcagaacggc cagcggccca
     1921 agagccagac caggcaccag cggacagccc gaggcgggcc gaaccggaga tggcggcgcc
     1981 tgccgacgcc gagcccaccc tttcccagca caccatcgag ccgacccgga ccagcatggt
     2041 gtggaccatg gtcggcatcg gggtgatcct cctggtcgcc atcctggtct tcatcctgca
     2101 gaacggc
//
LOCUS       DATADN010000124         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_315898, whole genome shotgun sequence.
ACCESSION   DATADN010000124 DATADN010000000
VERSION     DATADN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315898"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VHH53_01615"
     CDS             complement(<1..267)
                     /locus_tag="VHH53_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX2288910.1"
                     /translation="MHIPDGFVSAPVAVAGAAVAVAGLTICVRRAGRDGTERQLPLAG
                     LVAAFFLVMGAPIIPVAVGTSGHLLGGTLAVALLGPWLGPVVITV"
     gene            complement(254..997)
                     /gene="cobA"
                     /locus_tag="VHH53_01620"
     CDS             complement(254..997)
                     /gene="cobA"
                     /locus_tag="VHH53_01620"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX2288911.1"
                     /translation="MTVHLVGAGPGDPQLLTRRGAALLARADHVVYDRRSMADILALA
                     PSAIQHCVGKQGAIPATPQHEINKLLVQLGRTGACVVRLKAGDPFVVSRGGEEAVALA
                     CAGVDVEVVPGVSAAVAAPAAAGIPVMVRQLSRTLTVVAGNDGIYEVDWAAIARLGGT
                     VVLLTGRAALRRVIVALRAGGMPGDTPVAAISAATRPKQRTVRGTLDQPPDQRLAAPL
                     TVVIGEVARLDVARFDVTAGATGTSRAHP"
     gene            complement(994..>1500)
                     /locus_tag="VHH53_01625"
     CDS             complement(994..>1500)
                     /locus_tag="VHH53_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX2288912.1"
                     /translation="SSGDAGIYGMASPALTTAAALPAHRRPSVRVVPGITAAVAGAAQ
                     LGAPLARDFACLTLSDVLAPWDAVEARLRAVAVADLVLALYNPRSRGRSWQLQRARDV
                     LAEYRPGDTPVGLVTDAGRDGERVEVTTLARMDPATAGMHTVVIVGAAGTSRLGDWLV
                     TARSLLVT"
BASE COUNT          246 a          555 c          521 g          178 t
ORIGIN      
        1 cacagtgatc accaccggtc ccagccacgg tccgagcaat gccaccgcca aggtgccacc
       61 gagcagatgc ccgctggtac ccaccgcgac cggaatgatt ggtgctccca tgaccaggaa
      121 gaacgccgcg accagcccgg ccagcggcag ctgccgttcg gtaccgtcgc gtcctgcgcg
      181 acgcacgcag atcgtcagcc cggccacagc tactgcggca ccggcgaccg cgacgggagc
      241 ggagacgaaa ccgtcaggga tgtgcacggc tcgtcccggt ggcgccggcc gtgacatcaa
      301 accgggcgac gtccaaccgc gcgacctcgc ctatcaccac cgtcagcggg gcggcaagcc
      361 gctggtctgg cggctgatcc agggtgccac ggacggtgcg ctgcttcggc cgggtagcgg
      421 cgctgatcgc ggcgaccggg gtgtcaccgg gcattccgcc cgcgcgaagc gcgacgatga
      481 cccggcgcag tgccgcccgc ccggtgagta gcacgacggt gccgcccagg cgggcgatag
      541 cagcccagtc cacctcgtag atgccgtcgt tgccggcgac cacggtcagc gtgcgggaca
      601 gctgccggac catcaccggg atcccggccg ccgccggtgc ggctactgcc gcggacaccc
      661 cgggtaccac ctcgacgtcc accccggcgc aagccagagc caccgcctcc tcgccaccgc
      721 gggataccac gaaggggtcc ccggccttga gccgcaccac gcacgccccg gtccggccga
      781 gctggaccag cagcttgttg atctcgtgct gtggtgtggc ggggatggcg ccctgcttgc
      841 cgacgcagtg ctggatggcg gacggggcca gtgccaggat gtcggccatc gaccggcggt
      901 cgtagacgac atggtcggct cgggccagca gcgcggcacc gcgccgggtg agcagctgcg
      961 gatcaccagg tccggccccc accagatgca cggtcacgtg accagcagtg accgggccgt
     1021 caccagccaa tcaccgagcc ggctggtgcc tgcggcgccg acgatcacca cagtgtgcat
     1081 cccggctgtg gcaggatcca tcctggccaa cgtggtgacc tccacgcgct cgccgtctcg
     1141 ccccgcgtcc gtgaccagac cgaccggagt atcaccggga cggtactcgg caaggacatc
     1201 gcgagcccgc tgcagctgcc aggaccgccc tcgcgagcgc gggttgtaca gcgcgagcac
     1261 caggtcggca acggccaccg cgcgcagccg cgcctcaacc gcatcccagg gggccagcac
     1321 atcggacagg gtcaggcagg cgaagtcgcg ggccagggga gctccaagct gcgctgcgcc
     1381 agcgacggct gctgtgatgc cgggcaccac tcgcacactg ggacggcggt gcgcgggcag
     1441 cgcggcggcg gtggtcaacg ccggggacgc catgccgtaa atcccggcgt caccggagga
//
LOCUS       DATADN010000125         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1370797, whole genome shotgun sequence.
ACCESSION   DATADN010000125 DATADN010000000
VERSION     DATADN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370797"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VHH53_01630"
     CDS             <1..425
                     /locus_tag="VHH53_01630"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2288913.1"
                     /translation="DVRNTGAAFAAATAELSAQHWEATVQTRQGAVSAATIPWARARE
                     LWLHLVDLNSGATIDVLPEDVATALVRDVASWMGTRVPTRIELRILGGEAVTFGPETS
                     TPVPVCGSAQELAGWLTGRCQGDRLVAPAGLPDLPGWI"
     gene            complement(438..1181)
                     /locus_tag="VHH53_01635"
     CDS             complement(438..1181)
                     /locus_tag="VHH53_01635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX2288914.1"
                     /translation="MVTPPRWAGFSDRGRVRERNEDRWSADADLGLFIVSDGMGGRPA
                     GEVASEVVVAALPTLVAHHFGAEPDLAAPDAPQRLRTVLTELSTELREGSQDTPWLAG
                     MGATAVVALVSHTTALIAHVGDSRAYLLRGRSLHRLTQDHSLAQALIDAGAITDQQAA
                     GHPARGQLTRHMGMVGEVSPDACRVDLQSDDQLLLCSDGLTSMLDDAGIQAILNRSQD
                     PQHACRALTQAANDAGGTDNITVLVIAPA"
     gene            complement(1212..>1715)
                     /locus_tag="VHH53_01640"
     CDS             complement(1212..>1715)
                     /locus_tag="VHH53_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288915.1"
                     /translation="ATNASADPATDAKPADFTAFRNDQAGFELAYPTSWSKLDSKDPQ
                     VLLLVAKGTQDSFLIRSLELAKAVGPSELPAAKQLTDQIVTSNTSVQMITEPKQIELG
                     GLPGFFYFYSFKDAASGQEGVHSHYFLFNGNKMISLVFQVLPKDHFPASAPTFDKITA
                     TFRALKK"
BASE COUNT          282 a          554 c          590 g          289 t
ORIGIN      
        1 acgacgttcg caacactgga gctgcgttcg ccgcggccac cgcagagctc tccgcgcagc
       61 actgggaggc caccgtgcag accaggcagg gcgcagtatc cgctgccacc atcccctggg
      121 cacgggcgcg ggagctgtgg ctacatctgg tggatctcaa ctccggcgcc acgatcgatg
      181 tgctgcccga ggacgtcgcc accgcgctgg tgcgtgatgt tgccagctgg atgggaaccc
      241 gggtgccgac ccggatcgaa ctgcggatac tcggcggcga ggcggtcacc ttcggacccg
      301 agaccagcac ccccgtaccg gtctgcggat cggcccagga actggccggt tggctgaccg
      361 gccgctgcca gggcgatcgc ctcgtcgcgc cggccgggct gccagacctc cccggttgga
      421 tctgatcgca ggaccgctca cgcaggcgcg atcacgagca cggtgatgtt gtcggtgccg
      481 cctgcgtcgt tggccgcctg ggtaagggcg cggcaggcat gctgagggtc ctggctgcgg
      541 ttgaggatcg cttgtatccc agcgtcgtcg agcatgctgg tgaggccgtc gctgcagagg
      601 aggagctggt cgtcggattg caggtccacg cgacacgcgt cgggcgacac ctcgccaacc
      661 atgcccatgt ggcgggtcag ctggccccgc gcggggtgac cggctgcctg ctggtccgtg
      721 atcgcgcctg cgtcgatcag cgcttgcgcc agcgagtggt cctgggtcag ccggtgcagg
      781 ctgcgcccgc gtagcaggta agcgcgactg tcgccgacgt gtgctatcaa cgcggtggtg
      841 tggctgacca gcgcgacgac ggccgtcgcg cccatcccgg ccagccacgg cgtgtcctgg
      901 ctgccctcgc gcagttcggt actgagttcg gtgagcaccg tacgcagccg ctgtggtgca
      961 tccggggcgg cgaggtccgg ctcggcaccg aagtggtgag ccaccagcgt cggcagggcc
     1021 gcgacaacca cctcggaggc tacctcgccc gcgggccggc cacccatgcc gtcggacacg
     1081 atgaacaacc cgaggtcggc gtcggcgctc cagcggtcct cattgcgctc gcgcacccgg
     1141 ccgcggtcgc tgaaacccgc ccagcgtggc ggtgtcacca cgtcacagct cgtcgcgcaa
     1201 cgcgtgagtc gtcacttctt cagtgcacgg aacgtagcag tgatcttgtc gaaggtcgga
     1261 gcggacgcag ggaaatgatc cttgggtaag acctggaaga caagactgat catcttattg
     1321 ccgttgaaca ggaagtagtg ggagtgcacg ccctcctggc cggaggcggc atccttgaag
     1381 gagtagaagt agaagaagcc gggcaaccca ccgagctcga tctgcttcgg ttcggtgatc
     1441 atctgaaccg acgtattgga agtcacgatc tggtccgtca actgcttggc agcgggcaac
     1501 tcactcggcc cgacagcttt cgccaattcc agggagcgga tcaggaagga gtcctgcgta
     1561 cccttggcca ccaacaggag tacctgaggg tccttgctgt ccagcttgct ccacgaggtc
     1621 gggtaggcca actcgaaacc agcctggtcg ttgcggaagg cagtgaaatc agccggtttg
     1681 gcgtcagtcg ccggatccgc cgaggcattc gtcgc
//
LOCUS       DATADN010000126         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1069637, whole genome shotgun sequence.
ACCESSION   DATADN010000126 DATADN010000000
VERSION     DATADN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069637"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VHH53_01645"
     CDS             <1..912
                     /locus_tag="VHH53_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX2288916.1"
                     /translation="RESTLWVLFYVGLAALFGVGVWLASGPTYAGQFYAGWLTEYSLS
                     VDNLFVFVIIMSRFAVPRAYQQKVLLIGIVLALVMRGAFIAAGAALISQFSWVFYIFG
                     AFLVYTAITFFKQGEPDEDDFKENVLIRWSRRALPLSSTFDSGKMTVVTETGKRLFTP
                     MLIVMIAIGTTDLIFALDSIPAIFGITKEPYLVFTANVFALMGLRQLYFLLGGLLDRL
                     VYLNIGLAFVLGFIGVKLILEALHTNNLPFINGGEHVPWAPEIPIWLSLSVIIGTLGI
                     ATVASLVKSSRDAKREHLKQTVDSGIR"
     gene            complement(975..>1731)
                     /locus_tag="VHH53_01650"
     CDS             complement(975..>1731)
                     /locus_tag="VHH53_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX2288917.1"
                     /translation="EVQGRGELALAILVEQMRRENYELTVGKPQVVTRTVDGKVHEPV
                     ERLTIDAPDEYMGAITSLLASRKGRMEQLVNHGTGWIRMEWLVPARGLIGFRTEFLTE
                     TRGTGILHHLFEDYEPWFGDLRTRATGSLVADRAGVATAFAMMNLQERGTLFVEPGTE
                     VYEGMIVGENSRADDMDVNITKEKKLTNMRSSTAEETEKLIPPRRLSLEQALEFCRED
                     ECVEVTPAAVRIRKVVLDQQTRGRQAARRKHQQ"
BASE COUNT          241 a          593 c          569 g          328 t
ORIGIN      
        1 cgcgagagca ccctctgggt gctcttctat gtgggcctgg cggcgctgtt cggcgtcggc
       61 gtctggctgg cgtccgggcc cacgtacgcg gggcagttct acgccggctg gctgaccgag
      121 tacagcctgt ccgtcgacaa tctgttcgtc ttcgtgatca tcatgagccg attcgcggtg
      181 ccgcgggcgt accagcagaa ggtgctgctc atcggcatcg tgctggcgct ggtgatgcgc
      241 ggcgcgttca tcgcggccgg tgcggcgctg atctcccagt tctcctgggt cttctacatc
      301 ttcggcgcgt tcctggtcta caccgcgatc accttcttca agcagggtga gccggacgag
      361 gacgacttca aggagaacgt gctgatccgc tggtcgcggc gcgcgctgcc gctctcgtcg
      421 accttcgaca gcggcaagat gaccgtggtc accgagaccg gcaagcggct gttcaccccg
      481 atgctgatcg tgatgatcgc gatcggcacc acggacctga tcttcgcgct ggactccatc
      541 ccggcgatct tcggcatcac caaggagccg tacctggtct tcaccgcgaa cgtgttcgcg
      601 ctgatgggcc tgcgccagct gtacttcctg ctcggcggcc tgctcgaccg actggtctac
      661 ctgaacatcg ggctggcgtt cgtgctgggc ttcatcggcg tgaagctgat cctcgaggcg
      721 ctgcacacca acaacctgcc gttcatcaac ggcggcgagc acgtgccgtg ggcaccggag
      781 atcccaatct ggctgtcgct gtccgtcatc atcggcacgc tgggcatcgc gaccgtggcc
      841 agcctggtca agtcgtcgcg cgacgccaag cgggaacacc tgaagcagac cgtggactcc
      901 ggcatccgct gaccgcggcc ggcacagact tcgagggtac gggcacagcg cggtgcccgt
      961 accctcgttg cttcttactg ctggtgcttg cggcgggccg cctgccggcc ccgggtctgc
     1021 tggtcgagga ccaccttgcg gatgcggacc gcggccggcg tgacctcgac gcactcgtcc
     1081 tcgcggcaga actcgagggc ctgctccagc gacagccggc gcggcgggat cagcttctcg
     1141 gtctcctcgg cggtggacga ccgcatgttg gtgagcttct tctccttggt gatgttgacg
     1201 tccatgtcgt cggcccggga gttctcgcca acgatcatgc cctcgtacac ctcggtgccc
     1261 ggctcgacga acagtgtgcc gcgctcctgc aggttcatca tggcgaacgc ggtggccacg
     1321 ccggcccggt cggccaccag cgagccggtc gcgcgggtgc gcagatcgcc gaaccacggc
     1381 tcgtagtcct cgaacaggtg gtgcaggatg ccggtgccgc gggtctcggt gaggaactcg
     1441 gtgcggaagc cgatcagccc gcgggccggc accagccact ccatccggat ccagccggtg
     1501 ccgtggttga ccaactgctc catgcggccc ttgcggctgg ccagcaggct ggtgatggcg
     1561 cccatgtact cgtccggggc gtcgatggtc aggcgctcga ccggctcgtg caccttgccg
     1621 tcgacggtcc gggtgaccac ctgcggcttg ccgaccgtga gctcgtagtt ctcccgccgc
     1681 atctgctcca ccagaatggc cagggccagc tcaccgcggc cctgcacctc c
//
LOCUS       DATADN010000127         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_164960, whole genome shotgun sequence.
ACCESSION   DATADN010000127 DATADN010000000
VERSION     DATADN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164960"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            621..2138
                     /gene="istA"
                     /locus_tag="VHH53_01655"
     CDS             621..2138
                     /gene="istA"
                     /locus_tag="VHH53_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX2288918.1"
                     /translation="MKSARERMDILSAYREVGTYRGAAAMCGTTWKTVRRAVERHNAG
                     GAVPPRRARKRNYASVSELVAERVAATKARISAKRLLPAARRAGYEGSARNFRRLVAA
                     AKAEWRGGHHRGRRPAVWTPGEHLVIDWGVEGGLHVFCAVLAWSRVRFVRFAADERAA
                     TTFALLADCFETLGGVPKVVLADRMGCLKGGVVAGRVVPTPDYVRFATAFGFRPDFCE
                     AADPESKGIVEHLVGYAKSDLIVSQAPFEGGLVVANDAARSWCVEVNAAVHSEICAVP
                     AERLVGECELLSPLPSLRPELPGRTATTRKVDRLSCVRFGSARYSVPTRLIGSAVELR
                     SGQGRILVVEPGTGAVVADHALVAPGEASVFDEHYGSTRPAAPPRAVRPKTATEKQFC
                     ALGPVAQAFLTGAAAAGHTRLGAELGELLALEAAHGRAPLLAALTRATAFARWRASDV
                     RSILAAGAGAPNPRPAGEALVLELPVVPTRSLADYAVTPPAGDQPATAQRAGGAS"
     gene            2135..2917
                     /gene="istB"
                     /locus_tag="VHH53_01660"
     CDS             2135..2917
                     /gene="istB"
                     /locus_tag="VHH53_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX2288919.1"
                     /translation="MTATSVPELPADLAAGLRRLKMGAMRRLAPELLVTAKTQRWNPE
                     EFLRTLVEAEISARDASNARTRMRLAAFPVIKTVDQFEVCSSSVPPATFDYLASLEWV
                     RAAENTCLIGPAGTGKSHLLVALGVAAVHAGHRVRYFTAAELVETLYRGLADNSVGRV
                     IDTQLRNDLIIIDELGFAPLDDTGAQLLFRFVAAAYERRSLGIGSHWPFESWGRFLPE
                     HTTAVSLLDRLLHHCHTVITDGDSFRMKQARAGIGTTAKAAR"
     gene            complement(3063..3401)
                     /locus_tag="VHH53_01665"
     CDS             complement(3063..3401)
                     /locus_tag="VHH53_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288920.1"
                     /translation="MAVVQLRNPTEGRAFFDAKKAAGKTSMEAMRALKRRLSNVVYAR
                     MIADQRQRERQTRQGIRDDSAIQRDRPNPDVNGQVAVPAGGHEKVPTPRGFDHMCSLR
                     CSAVRQAGLQ"
BASE COUNT          575 a         1263 c         1307 g          618 t
ORIGIN      
        1 gtgtcgcgca gccacagttg ggcggcgcgt agcacccgag taagccttca gtacctcctc
       61 ggcccgacac taccggcgtg gtcgggaggg gctgaccgac caagatcggt gagtgttcag
      121 gccgtcagac gtcaagtcgc gtcgggatac aacaggacaa acaagagcaa accagcgggt
      181 cagaataggt ccgccgtcag acatcgcaag cacggcgtcc aggctaagaa aagtcgggct
      241 tctcaggggc gagcggcaga tcgtcacacg gtgatcccgt cgtccccatg aggagcctgg
      301 tcgaaaacac ttgacccaga atcagatgtt tggcagcaga cagaccgccg gtcagcaccc
      361 tccaccgaac agcctcgtgg cctacatcag tcgatacatc aacggctacg gaccaccatg
      421 gacaccctcg gacgtggacc caaggatcag gctcgttgtc cgtggccatg gaaaagtccc
      481 cgctgatggc caggtgtagg tccccgctgg tggccaggta gaagtcccca tccctcgctc
      541 gtcgtgtcga cccggagtgt gggccctggg cggtgacggt ggcggtgcca accagtcacc
      601 gcaccgccca gggggctcca ttgaagtctg cgagggaacg gatggacatc ttgtcggcct
      661 atcgggaagt gggtacctat cgtggcgccg ccgcgatgtg cggcaccacg tggaagacgg
      721 tgcgccgcgc ggtggagcgg cacaacgccg gcggggcggt tccgccgagg cgggcgcgga
      781 agcgcaacta cgcgtcggtg agtgagttgg tcgccgagcg ggtcgcggcg acgaaggccc
      841 ggatttcggc caagcggctg ctgcccgcgg ctcggagggc ggggtatgag ggctcggcac
      901 ggaacttccg ccggctggtc gccgcggcca aggccgagtg gcggggcggg catcaccggg
      961 gtcggcgtcc ggcggtgtgg acacccggtg agcacctggt gatcgactgg ggagtcgagg
     1021 gtggactgca cgtgttctgc gcggtgctgg cctggtcgcg ggtgcggttc gtgcgtttcg
     1081 ccgccgacga acgcgccgcc acgacgttcg cgctgctcgc cgactgcttt gagaccctgg
     1141 gtggggtgcc caaggtggtg ttggccgacc ggatggggtg tctgaagggt ggggtggtcg
     1201 ccgggcgggt ggtgccgacc ccggactacg tccggtttgc caccgcgttc gggttccgcc
     1261 ccgacttctg cgaggccgca gatccagagt cgaaggggat cgtggagcac ctggtcggct
     1321 acgccaagtc cgacctgatc gtctcgcagg caccgttcga ggggggtctg gtcgtggcca
     1381 acgacgccgc ccggtcctgg tgtgtggagg tcaacgccgc ggtgcactcg gagatctgcg
     1441 cggtccccgc cgaacggctg gtcggcgagt gtgagttgct ctccccgttg ccctcgctgc
     1501 gaccagaact gcctggtcgc accgcgacga cgcgcaaggt ggatcggctg tcgtgtgtgc
     1561 ggttcggctc ggcccgctac tcggtgccga cccggctgat cgggtccgca gtggagctcc
     1621 gctcgggcca gggccggatc ctggtcgtgg aacctggcac cggcgcggtc gtcgccgacc
     1681 atgcgctggt cgccccgggc gaggccagtg tgttcgatga gcactacggc agcacgcgcc
     1741 cggccgcccc accgcgcgcg gtgcgtccaa agaccgccac ggagaagcaa ttctgcgcgc
     1801 tgggcccagt cgcgcaagcg tttttgaccg gagcggccgc ggccggacac acccgcctgg
     1861 gagccgagtt gggtgagctg ctcgccctgg aggccgcgca cggccgcgcg ccgctgctgg
     1921 ccgcgctgac ccgggcgacg gcgttcgcgc gctggagggc cagcgatgtg cgctcgatcc
     1981 tggcggccgg ggcgggcgcg cccaatcccc gcccggccgg cgaggcgctg gtccttgagc
     2041 tgccggtggt cccgacccgc tcgttggccg actatgcggt gactccaccg gccggtgacc
     2101 aaccagccac cgcccagcgc gccggtggtg cgtcgtgacc gcgacctcgg tcccggagct
     2161 gcccgcggac ttggccgccg ggctgcgtcg gctcaagatg ggcgcgatgc gccgtctggc
     2221 cccggagttg ctggtcaccg cgaagaccca acgctggaac cccgaggagt ttctccgcac
     2281 gctggtcgag gccgagatca gcgcccgcga cgcctccaac gcccgcaccc ggatgcggct
     2341 ggccgcattc ccggtgatca aaaccgtgga ccagttcgag gtatgttcct cctcggttcc
     2401 acccgccacg ttcgactacc tcgcctccct ggaatgggtc cgggccgcgg agaacacctg
     2461 cctgatcggc ccggccggca ccggcaagag ccacctactc gtcgcgctgg gcgtcgccgc
     2521 cgtgcacgcc gggcaccggg tccgctactt caccgccgcc gagctggtcg aaaccctcta
     2581 ccgaggccta gccgacaact ccgtcggccg cgtcatcgac acccagctcc gcaacgacct
     2641 gatcatcatc gacgaacttg ggttcgcccc gctggatgac accggcgcgc agctgctgtt
     2701 ccgcttcgtc gctgccgcct acgaacgccg ctccctgggc atcggctcac actggccctt
     2761 cgaatcctgg ggacgattcc tccccgaaca caccaccgcg gtcagcctgc tcgaccggct
     2821 cctgcaccac tgccacaccg tgatcaccga cggcgactca ttccgaatga aacaggcccg
     2881 agccggcatc ggcaccaccg ccaaggccgc tcgatgaccg agcatcttga atatcgttac
     2941 aacccgaacc ggtcatcggg cgcctgcccg gaccaccccg gctgcctcat cctgcgctgc
     3001 cgccgctggc tctgcccgcg caacttccac accccgatca accagcgcgg acaaccccgc
     3061 cgctactgca gccccgcctg ccgcaccgcc gaacaccgga ggctgcacat gtgatcaaac
     3121 ccccgagggg tggggacttt ttcatggcca ccagcgggga ccgcaacttg gccattgaca
     3181 tcggggttag gccggtcacg ctggattgca gagtcgtccc gtatgccctg ccgggtctgc
     3241 cgctcgcgtt gtcgttggtc ggcgatcatg cgggcataga cgacattgga gagccgccgt
     3301 ttgagtgcgc gcatggcctc catcgaggtt ttcccggcgg cttttttggc gtcgaagaag
     3361 gcgcggcctt cggtgggatt gcggagttgc acgacggcca tgatttgcag gacacggttg
     3421 attctgcgat tcccggctcg ggagaggcgg tggcgctggt gttcgccgga ggaggcgtcg
     3481 aggggggcga tgccgtttgc aggaggcgaa ccggtcccgg ttgcggaatc ggtggatgtt
     3541 tccgacatcg gccagtaatc gcgccgtgct ggagggaccg atgccgtgca ggtccattag
     3601 ggtggagccg cggtggatga ccagctgctg aagttccttg tcagcatcct tgatcttgcg
     3661 gtcgatgctg gagagctcgc tgatcaactc gaccacgagc cggcggcggg tcttgccgac
     3721 cagatcgcgc ggtttgatcg tggcgatgag cgcgcgggcc tgg
//
LOCUS       DATADN010000128         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_617765, whole genome shotgun sequence.
ACCESSION   DATADN010000128 DATADN010000000
VERSION     DATADN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617765"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VHH53_01670"
     CDS             complement(<1..317)
                     /locus_tag="VHH53_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
                     /protein_id="HEX2288921.1"
                     /translation="MQVREQAVGCPIGLVGCGFSPPDALATLADHVSWPGLFLADESR
                     RLYGLLGMRRARLWQVYSLGTLARYALAAARGQQLLRPVEDIRQMGGDALLQDGVVVR
                     RWLP"
     gene            complement(339..470)
                     /locus_tag="VHH53_01675"
     CDS             complement(339..470)
                     /locus_tag="VHH53_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288922.1"
                     /translation="MPREVDQPVPLDGLALPDARTGQTVDLGALPGSYLLTAIRHRY"
     gene            complement(460..>1721)
                     /locus_tag="VHH53_01680"
     CDS             complement(460..>1721)
                     /locus_tag="VHH53_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288923.1"
                     /translation="HAVGCEGTHGVFPRCNLACTPCYHSRDANRIRVDGSHTVAEVGK
                     QMALLRRLRGPRAHAQLIGGEVTLLPADDHAETLLTMRRYGREPMSMSHGDFDYDYLE
                     RLALDGQGRRRLARLSFAGHFDMFMFGRRGIPRPPSERELNPYRQRFVEMFKNLRARH
                     GVRFFLAHNMTVTPANLGQVAELVRDCHAMGFGMFSFQPAAFVGDDRRWHEDYEQVGM
                     DQVWQEIEKGVGTTLDYTVIQHGDLRCNRAAYGFFVGRCWHPFLSGDDPRDLATREAF
                     FRYLGGTNFTGVEAPDLIGKLVRTIVRHPVLVVIALQWIARTLRRAGGVRALLRHGVR
                     PVSFVVHRFMDAADVTSAWELMQRGEKASDPRILATQERLASCHYAMAHPETGKLVPA
                     CVQHSVLDPIENIELRRLLPIVDVRAT"
BASE COUNT          294 a          593 c          574 g          260 t
ORIGIN      
        1 ggcaaccacc ggcgcacgac gacgccatcc tgcagcagcg cgtcgccgcc catctgccgg
       61 atgtcctcta cgggccgtag cagctgctgc ccgcgcgctg cggccagcgc gtagcgggcc
      121 agagttccca gcgagtacac ctgccacagc ctggcacggc gcatgccgag caacccgtag
      181 agccggcggc tctcatcggc caggaacaga cccggccagc tcacgtggtc ggcgagcgtg
      241 gcgagcgcgt caggtgggct gaatccgcac ccgaccaagc cgatcgggca accaacagcc
      301 tgctcgcgca cctgcacgag gtgctgctgg cagggcagtc aataccggtg ccggatcgcg
      361 gtgagcaggt agctgccggg cagcgcgccg aggtccacgg tctggccggt gcgcgcatca
      421 ggcagcgcca acccgtcgag agggacgggc tggtcgacct cacgtggcac ggacgtcgac
      481 gatgggcagc aaccggcgga gttcgatgtt ctcgatcgga tccagcaccg agtgctgcac
      541 gcaggcggga acgagtttac cggtctcggg atgggccatc gcgtaatggc aggacgcgag
      601 ccgttcctgg gtggcgagga tgcgcggatc ggaagctttc tcgccacgtt gcatcagctc
      661 ccacgccgac gtgacgtccg cggcgtccat gaaccggtgc accacgaacg ataccggtcg
      721 cacgccgtgg cgcagcagcg cccgcacccc accggcgcgg cgcagggtac gcgcgatcca
      781 ctggagcgcg atcaccacga ggaccggatg gcgcacgatt gtccggacca acttgccaat
      841 caggtccgga gcctccacgc cggtgaagtt ggtcccaccg aggtagcgga agaaggcctc
      901 cctggtggcg agatcacgag ggtcgtcacc ggacaggaac gggtgccaac atcggcccac
      961 gaagaatcca tatgctgccc ggttgcaccg caggtcgccg tgctggatca cggtgtagtc
     1021 cagcgtggta cccacgccct tttcgatctc ctgccagacc tgatccatcc cgacctgctc
     1081 gtagtcctcg tgccagcgcc ggtcgtctcc gacgaacgct gccggttgga aggagaacat
     1141 gccaaacccc atcgcgtggc agtcccgcac cagctccgcg acctgaccga ggttggccgg
     1201 ggtgacggtc atgttgtgcg ccagaaagaa gcgcaccccg tgcctggcgc gtaggttctt
     1261 gaacatttcc acgaaccgct gccggtacgg gttgagttcc cgttcgctgg gtggtcgcgg
     1321 gatgccgcgc cggccgaaca tgaacatgtc gaagtgcccc gcgaacgaca gccgcgccag
     1381 ccggcgccgt ccttgcccat ccaacgccag ccgctccagg tagtcgtagt cgaagtctcc
     1441 gtgggacatg ctcattggtt cgcgcccgta ccggcgcatc gtcagcagcg tctccgcgtg
     1501 gtcgtcggcc ggcagcagcg tcacctctcc gccgatcagc tgagcgtggg cgcggggacc
     1561 gcgcagccgg cgcagcaacg ccatctgctt gccgacctca gcgacggtgt gtgacccgtc
     1621 tacgcgtatc cggttggcgt cacgcgagtg gtagcagggc gtgcatgcca ggttgcagcg
     1681 cgggaacacc ccgtgggtac cttcgcagcc caccgcgtgc c
//
LOCUS       DATADN010000129         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_165004, whole genome shotgun sequence.
ACCESSION   DATADN010000129 DATADN010000000
VERSION     DATADN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165004"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..703
                     /locus_tag="VHH53_01685"
     CDS             209..703
                     /locus_tag="VHH53_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX2288924.1"
                     /translation="MGVEYGSVKWKGMDMVLADPEVRFGIRLRKPGPRTLDQMYSLII
                     GLVVIVLGIVGFLVTGFENFTEMTDQQLLGLLHMNGFHNLVYIILGVWWLMGAFALTP
                     AGNQGLNIAMGGALALVAVLGALGYMSLLSIPGGITPNTILLLAVAVATLIFGGGLLS
                     RGGR"
     gene            complement(1027..2652)
                     /locus_tag="VHH53_01690"
     CDS             complement(1027..2652)
                     /locus_tag="VHH53_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2288925.1"
                     /translation="MLVLVALMAPGHGPLASRALLDPVVWWYVARASGLLAWALLGAS
                     VVGGLLLSTRLARGHTRTWTQGLHQFVGALAVIFTVLHLVSVLAADELRIGLAELLVP
                     FVRSDNPVAQGCGVLAFYLLIAVVLTSWARALLPWRRWRRLHLLAFPLFALTCAHTVL
                     AGSDITEPIVHWASLAVGILIFFLVIFRLLTARAAGTAAAAPAGLAQAAILASLDPPR
                     PTTHTPTPALVTVGTGMRLLITQTSWEADHVLSLLLRSPDGTALPSWEPGAHIELALP
                     SGRRRQYSLCGDPNDTCCYRIAILQVAEGRGGSVEVHTRVRAGQLLTVQGPCNHFPLV
                     ASPAYLFIAGGIGITAMLAMTARVAAAGAEWKLVYTGRRRTGMAFIDEVLARDPERVD
                     VVPNDERGRPDLGKIIGAAAPGTAVYCCGPDRLLHDVRERVTARPDLSLHSQRFTGTT
                     ASGGAALRVELRRTGRIIDVPANRSVLQAVRDVLPAVPAGCEQGICGGCRTTVLAGEP
                     DHRDEMLNSAERAAGQMLICVSRARSQRLVLDL"
BASE COUNT          644 a         1010 c         1039 g          537 t
ORIGIN      
        1 ggtatcccat cgcgtcgacg ccagaggctt gcgcgtagat ctccatggtc accgaaattt
       61 gggcgtgagg cagcgctgaa gtgactctgt cttgcacaaa cccccgaccc ggccaacaat
      121 gccaatacac gcgcagctat caccggtcgg tgccccaggt ggtctgtcca gctgggtcgc
      181 ccaggttcgc tgtgtagcgc ccggcgcggt gggcgtcgag tacggcagtg tcaagtggaa
      241 ggggatggac atggtattag ctgatccaga agtcaggttc gggatcaggc tccgcaagcc
      301 ggggcccaga acgctcgacc agatgtattc cttgatcatc ggactcgtcg tgatagtcct
      361 tgggatcgtc gggttcttgg tgacagggtt cgagaacttt accgagatga ctgaccagca
      421 gcttctcggg ctcttacata tgaacggctt ccacaacctc gtgtacatca tccttggggt
      481 gtggtggctg atgggggcgt tcgcgctgac ccctgcaggc aatcaggggt tgaacatcgc
      541 catgggtggg gccttagcgc tggtcgccgt gttgggcgct ttgggctaca tgtctttgct
      601 gtccatcccc ggcggaatca ccccgaatac catcctgctc ctggcggtcg ctgtggcgac
      661 cctaatattc ggcggtggcc tgctcagtag gggcggtcgt taacccgcta ccgcgtaggc
      721 gtaagaggcg aaactcatgg gccgtgttct ctgaccggca gagaacccag ccaagccgcg
      781 tagctagcga atcagcgaca cgggaaaatg ggcatgcctg acacaggcta gacaacaggt
      841 atgggtcctg ggggcgctcc acagcagccg tgaatgggat tgctcagcgc agtcgcagca
      901 cggctgccca gggcccaggt agcccagtta tcaacagggg tggcttgacg tccaggtcgg
      961 ctagtgcatc gatcgatcgg ccacggccca gcacttgctg ccgacagttc acaccaagag
     1021 gcgtcatcac aggtcgagga cgagccgctg gctgcgggcg cgagagacgc agatcagcat
     1081 ctggccggct gcgcgctccg cgctgttgag catttcatcg cggtggtcgg gctcaccagc
     1141 gaggaccgtg gtgcgacagc cgccacagat gccctgttca catccggctg ggacggcggg
     1201 aaggacgtcg cggactgcct gcaaaacgct gcggttggcg ggcacgtcga tgatgcgtcc
     1261 ggtgcggcgc agctcaacgc gcaaggcggc gccgccactg gcggtggtgc cggtgaagcg
     1321 ctgactgtgc aggctgaggt ccggccgggc ggtgacccgt tcccgcacgt cgtgcagcag
     1381 ccggtccggg ccgcagcaat agacagcggt gccgggcgct gcagcaccga taatcttgcc
     1441 caggtcggga cgaccacgct cgtcattggg cacaacgtcg acccgctcgg ggtctcgcgc
     1501 aaggacctcg tcgatgaagg ccatgccggt gcgccggcgc ccggtgtaca ccaatttcca
     1561 ctcagcgccc gcggcggcca cccgggcggt catcgccagc atggcggtga tccctattcc
     1621 gccggcaatg aataggtagg ccgggctggc aaccagaggg aaatggttac atggcccttg
     1681 cacggtaagc agctgcccgg cccgcacgcg ggtgtggacc tccacggatc caccgcggcc
     1741 ctcagcgacc tgcagaattg caatccggta gcagcaggtg tcgttcgggt cgccgcatag
     1801 cgagtactgc cggcgccggc ccgagggcaa agccagctcg atatgggctc cgggttccca
     1861 gctggggagc gctgtcccgt ccggtgagcg caggagcaat gacagcacgt ggtcggcctc
     1921 ccagctcgtc tgggtaataa gcagccgcat cccggtcccc accgtaacga gagccggagt
     1981 aggggtatga gtggttggcc gcgggggatc gagggatgca agtattgctg cctgcgcgag
     2041 gcccgcaggc gccgctgccg cagtaccggc cgctcgcgcg gtgaggagcc ggaaaatcac
     2101 caggaaaaaa atcagtatcc ctaccgccaa gctggcccag tgcacgatcg gctcagtgat
     2161 atcgctgccg gccagcacag tgtgtgcgca ggtcaaggcg aataacggaa acgccagtag
     2221 atgcaggcgg cgccaccgcc gccagggcag caaagcccgc gcccaggaag tcagcaccac
     2281 cgcgatgagc aggtaaaagg caagcacccc acaaccctga gccactggat tatccgacct
     2341 gacaaacgga acgagcagct cagccaggcc gatccgcaac tcatcagcgg cgagcacgga
     2401 gaccaagtga agcacagtga aaatcaccgc aagcgccccg acgaactggt ggagtccctg
     2461 tgtccaagtg cgcgtgtgcc cccgggccag ccgcgtggac agcagcaggc cgcccaccac
     2521 cgatgcaccg agcaaagccc aggctaaaag acccgacgcc cgggccacat accaccacac
     2581 caccggatca agcagagctc gcgaggcgag aggaccgtgg ccgggcgcca tcagcgcgac
     2641 cagaaccaac atggcggctg cgctaagacc cctgcccaga tccgcgacgc accggtggct
     2701 cgaaccagca catcagtcac cgctgccctt ggcactaacg gcgtggtccc cttgatctcc
     2761 tttacgttgc gatctggcgg ttggtgccgt ccggatcctt tgctttgtgg aagcgacaac
     2821 caccttggtt tcgcgcgagg gcacctcacc ggtgggtatc agtatgtgca gccacaattg
     2881 cgcagcgccg taggcggcat gagcatcgtc gtcgctgtgt ccgcaatccg taccgggccc
     2941 atagcacagt gtcatccacg cccctcatgc cgcgaagacc tgtttccgct ctggcgatca
     3001 cagggtaagg ctcacgcgaa ccagcatggg cgtgaaccag aacatccaaa ggatgaatta
     3061 acgaagcctt ctgtagtaac gcggaccatc gcaaccgtga tagctgatga cacgatagtg
     3121 tgcaactgtt acgtagtcgg cgcctcgggt aggcaaacag gcaggagatc gctggccttt
     3181 cgttactgct cgcgagcagt gtgtacatca gacccgagaa ctacgtagcc
//
LOCUS       DATADN010000130         3987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1370985, whole genome shotgun sequence.
ACCESSION   DATADN010000130 DATADN010000000
VERSION     DATADN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370985"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..955)
                     /locus_tag="VHH53_01695"
     CDS             complement(8..955)
                     /locus_tag="VHH53_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2288926.1"
                     /translation="MVTNVPGMHPPLIDTPARPLPGEQRAGRRDRRLSRRRLYLRRFL
                     RNRGAVAGLVILVLLVLFGLFGGLFTPYTHTDVDFTNLTAPPFGEHWFGTNGAGNDTF
                     AQAVHGLQRSLVIAVVVSLLTTVISAFAGAAAAYFSGTAEKFILGIVHFLLVVPSFLI
                     LALVSQKAGGDWKVLILALTVFGWMLGARVVWSLAASLREREYVQAARFMGVGSVRVV
                     LRHLVPNIGSLLIVSFTLGVVATVQAETALSFIGFGVQLPDVSLGTMLSDGANTITSA
                     PWLFYFPAGLVTLLTVSMALIGDGLRDALDPTSQSGGRA"
     gene            complement(955..1941)
                     /locus_tag="VHH53_01700"
     CDS             complement(955..1941)
                     /locus_tag="VHH53_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2288927.1"
                     /translation="MLRYLVRKSLGWLLMIVVATNLTYFLANFFLDPRANYLQLRPPR
                     TPEQINRSLDQYNLSPETPLLERWWHWISGIVLRWDWGSSPIGESVNGQIGFRIGVSA
                     QLVLFALILSTTIGIALAVYTASRQYRFADRFYQGLSIITLNIPIVVAGLAVVLLGIA
                     LNQSLGTTLLFVAGAQSPGVSGFWPLLVDRLQHLVLPTVALVMTSYAGYHMLQRSLLL
                     DNINSDYVRMARAKGLTRQQAIRRHGLRTSLIPVATSIAFAIPALFTGAVITETIFAW
                     EGMGRYFVTTISKNDVHGAVAVAAFGALMTAIGAILADIAVVALDPRVRVSS"
     gene            complement(2156..2464)
                     /locus_tag="VHH53_01705"
     CDS             complement(2156..2464)
                     /locus_tag="VHH53_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288928.1"
                     /translation="MRHLMQTDYPDLVIAKRLLDHLKALGFQFQRAALGEDGPLVGYR
                     ISDDYLDLVRIEGFSRDCFAWRKRASSLIVPGNGLVERRSDGSALNVLNDVLTWQTLV
                     "
     gene            3083..3619
                     /locus_tag="VHH53_01710"
     CDS             3083..3619
                     /locus_tag="VHH53_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288929.1"
                     /translation="MAEPAEPRTLLEALVRQRQLSWDEAADLVVRTAKKHEDIGVSLS
                     GRHLGRLARGERSSNRPNPVTRRALQYTFHRPIDELLGPYTPRDLPVIDELKHGPESS
                     EVLTAATDRARRFMTSLQTLSDESLQLIHDEVRDLVRAYPARPLPEILGHLVSCQETV
                     FSLLERHSGQPTPGSSIS"
     gene            3625..>3987
                     /locus_tag="VHH53_01715"
     CDS             3625..>3987
                     /locus_tag="VHH53_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX2288930.1"
                     /translation="MLGGLLAKASHDLADPHAALAQSRTAWLCAEQANHDGLRAWISG
                     LQALICYWARRPHDSVRYAQRGTAYAQRAGNSALVWLPVSEACARAALGHGEHALDKI
                     ERAEEAWRQVRPDELDEMG"
BASE COUNT          755 a         1317 c         1312 g          603 t
ORIGIN      
        1 ggttcggtca cgctcgcccc ccggactggg acgtcgggtc gagcgcgtca cgcagtccgt
       61 caccgatcag cgccatcgag acagtcagta gcgtgaccag gccagccggg aagtaaaaca
      121 accacggggc gctcgtgatc gtgttggccc cgtcgctgag catcgtgccc agcgagacgt
      181 cgggcagctg gactccgaag ccgatgaacg acagcgcggt ctcggcctgc acggtggcga
      241 ccaccccaag ggtgaacgac acgatcaaca gcgagccgat gttggggacc aggtgacgca
      301 gcaccacgcg cactgagccg acgcccatga accgggcggc ctggacatac tcccgctcgc
      361 gcagagaggc ggccaacgac cacaccacgc gtgcgccgag catccagccg aagacggtca
      421 gcgcgaggat gagcaccttc cagtcgccgc cggccttctg cgagaccagc gccaggatca
      481 ggaacgatgg cacgaccagc agaaaatgca caatcccgag gatgaacttt tccgccgtac
      541 cgctgaagta ggccgcggcc gcacccgcga acgccgagat gaccgtggtg agcagcgaca
      601 ccaccaccgc gatcaccaac gaccgctgca ggccgtgcac ggcctgggcg aaggtgtcgt
      661 tgccggcgcc gttggtaccg aaccagtgct cgccaaatgg tggcgcggtg aggttggtga
      721 agtcgacgtc ggtgtgtgtg tagggggtga acagtccacc gaacaggccg aacagcacca
      781 gcaggaccag aatgaccagc ccggccaccg cgcctcggtt gcgcaggaaa cggcgcaggt
      841 acagccgccg ccgggacagg cgccggtcgc gacgcccggc ccgctgctcg ccgggcagtg
      901 gcctcgccgg ggtgtcgatg agcgggggat gcatcccggg cacgttggtg accatcagct
      961 gctcacccgc actctcggat cgagcgccac cacggcgatg tcggccagga tcgcgccgat
     1021 cgcggtcatc agcgcgccga aggcggcgac ggcgaccgcg ccgtggacgt cgttcttgct
     1081 gatcgtggtg acgaagtacc gtcccatgcc ctcccacgcg aagatggtct cggtgatcac
     1141 ggccccggtg aacagcgccg ggatcgcgaa cgcgatggag gtggcgaccg ggatcagcga
     1201 cgtgcgcaag ccgtgccgcc ggatggcttg ctgccgggtc agacccttgg ccctggccat
     1261 ccgcacatag tcggagttga tgttgtccag tagtagcgac cgttgcagca tgtgataacc
     1321 agcgtaactg gtcatgacca gcgccaccgt cggcagcacc aggtgctgga gccgatccac
     1381 cagcagcggc cagaacccgc tcaccccggg agactgggcg ccggcgacga acagcagggt
     1441 ggtgcccagc gactggttga gcgcgatccc cagcagcacg accgccaggc cggcgaccac
     1501 tatcgggatg ttcaacgtga tgatcgacag gccctggtag aaccggtcgg cgaagcggta
     1561 ttggcgggag gcggtgtaca ccgcgagtgc gataccgatc gtcgtggaca ggatcagcgc
     1621 gaacagcacg agctgcgcgc tgaccccgat ccggaagccg atctggccgt tgacgctctc
     1681 cccgattggt gacgagcccc agtcccagcg cagcacgatg ccgctgatcc agtgccacca
     1741 gcgttccagc agaggcgtct cggggctgag gttgtactgg tccagcgacc ggttgatctg
     1801 ctcgggcgtg cgcgggggcc gcagctgcag atagttggcc cgggggtcca ggaagaagtt
     1861 ggccaggaag taggtcaggt tggtggccac cacgatcatg agcagccagc caagagactt
     1921 cctgacgaga tacctcagca cgaccgtacc gctccggcca tgggcgcccc gatctgctcg
     1981 tctcacaccg acgtgtccgc cgcgccccct cgcagacccg cggaagtata aggcgggcat
     2041 cgaataggca gaccggctgg tggccggcgc ggaggcgacg agcccgcatc cgccggctgc
     2101 tcaccgaccg ctgccatggt gtcgccgccg tgtgccgttg acgacgctgg gctggctaca
     2161 ccagcgtctg ccatgtcagc acgtcgttga gcacattgag cgcactgcca tccgaccggc
     2221 gctcgaccag tccgtttcct ggaacgatca acgacgatgc ccgctttcgc caagcgaagc
     2281 aatcgcgact gaatccttcg atgcggacga gatccaggta gtcatcgctg attcggtacc
     2341 ccactagcgg gccatcctcg ccgagagcag cgcgctggaa ctggaatcca agcgctttca
     2401 aatgatcgag tagtcgcttg gcgatcacca gatccgggta atcagtctgc atcagatgcc
     2461 tcatcgaacc gctccgcgcg ccacttcacc ggtacggttg attgggacac ggcggaaccg
     2521 gaggctgtcg ggtgcacccc ggctccgccg tgagtgggat cggccgtcgc gccaggaatg
     2581 cccggacgtc ggacggccag ggtctgagat ggctggccct gctcggggga aggtcggtcg
     2641 ttcgcaatcc gcggacgaac gtggaacgca gaaactctgc gggctgctgc agccagaagt
     2701 ccgccttcat gtgcactcgg cagaggcgac gacgtaaccc gaacgctgcg cccgaacgcc
     2761 gacacgaccg gcaccgaccg tgccgatcca ccgagtggtc caccagcagg aagtacagcg
     2821 ccgcgctcgc ccggtacgga gccgaaccaa tccatccgcc gcaccaggag ctgactccac
     2881 cgcggcacgc agaataatgc tcagcacctc gcctgcccga gctgtcttca cgacgccaaa
     2941 ggattcgcta cctagcgtga ccacgcgatg cccgcggttg gacagcgccg gacctccacc
     3001 ggacatctcc ggaccggtgc ttgcacccgg gtatgccagg cggaattgtc gggctggtca
     3061 ccgtggaggg ggagattgac tgatggccga accggcagag ccacggactc tgctggaggc
     3121 gctggtgcgt cagcggcagc tgtcgtggga cgaagcggcc gacctcgtgg tgaggacggc
     3181 taagaaacac gaggacatcg gcgtctcgct cagtggccgt catctgggac gcctggctcg
     3241 tggcgagcga tccagcaacc ggcccaatcc ggtcacccgc cgggcgctcc agtacacctt
     3301 ccaccggccg atcgatgagc tcctgggtcc ctatacgccc cgcgacctcc ctgttatcga
     3361 cgaactaaag cacggtccag agtcgtcgga ggtgctcacc gcggcgaccg accgggcacg
     3421 acgatttatg acgagtcttc agacactgtc cgatgagtcg ctgcaactga ttcatgacga
     3481 ggtgcgcgac ttggtgcgcg cttatccggc gcggccgcta ccagaaatcc tgggtcacct
     3541 ggtctcttgt caagagacag tattcagcct gctggagcgg cacagcggcc aacccacgcc
     3601 cggcagctct atttcttgag cgcggtgctc ggcgggcttc tggccaaggc gtcacacgac
     3661 ctggccgatc cgcatgcagc gctggcacag tcgcgcactg catggttgtg tgccgagcag
     3721 gccaaccatg atggcctacg ggcttggatc tccggacttc aggcgctgat ctgctactgg
     3781 gcgcgccgac cccatgacag cgtccgctac gcccagcgcg gcactgctta cgcccagcgt
     3841 gcaggcaaca gtgcactggt gtggctgccc gtgagtgagg catgcgcgcg ggctgctctc
     3901 ggccacggtg aacacgccct agacaagatc gaacgtgccg aggaagcttg gcgccaggta
     3961 cgcccggacg aacttgatga gatgggc
//
LOCUS       DATADN010000131         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_467010, whole genome shotgun sequence.
ACCESSION   DATADN010000131 DATADN010000000
VERSION     DATADN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467010"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..920)
                     /locus_tag="VHH53_01720"
     CDS             complement(267..920)
                     /locus_tag="VHH53_01720"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2288931.1"
                     /translation="MNLWRSSSLHPGWPQPAGPLRVSAGVVRLRPVRLRDGAQWSRLR
                     LAERDHLERWEPATGLDWATRHAIMSWPSVCSGLRSEARKGRMLPYTIELDGQFCGQL
                     TIGNVTHGALRSAWIGYWVAKAVNGGGVATAALALGVDHCFGLVRLHRVEATVRPENA
                     ASRAVLAKVGFREEGLLRRYLEVDGAWRDHLLMAITIEELDGSAAAALVRQGRASWA"
     gene            complement(940..>1541)
                     /locus_tag="VHH53_01725"
     CDS             complement(940..>1541)
                     /locus_tag="VHH53_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX2288932.1"
                     /translation="RDAGAEVNRVGIVDTDPKRLREVVEGQINRAEVVVIAGAVGGAA
                     AEAVRSVLADLGEMGVTRIAMHPGSVQGFGQLGRDGVPVFLLPANPVSALVVFEVMVR
                     PLIRLSLGKRQAHRRIVQARALSPIESVAGRKGYLRGQLMRDQDTGEYLVQALGGAPG
                     SSHLLATLAEANCLVVVPTEAQQVRTGEIVDVAFLAQRG"
BASE COUNT          271 a          585 c          468 g          217 t
ORIGIN      
        1 ggattggggg atgcttggca tgtcgcctgc tcctttcccc gtaaaggcta ggtctgtgac
       61 ctgcgcatcc gtgggcggcg cgccgagaca attacacacc tgtaattcat gcatgacaag
      121 caccaccagt aacaaatgtc acattggcaa caacggatgc tgcctcgctc ccgcgagatc
      181 tacaccaggg tcgctgtttt gctcaccgaa cagccctccg gtagatctcg cgaaaagctc
      241 aacaagacaa gcggaaacaa ccgacgctat gcccagctcg cgcggccctg tcgcaccagc
      301 gcggcggccg ccgacccgtc gagttcctcg atcgtgatgg ccatcagcaa gtggtcgcgc
      361 caggcgccgt cgacctcgag ataccggcgc aacagccctt cctcccgaaa gccgaccttc
      421 gccagcaccg cgcggctcgc cgcgttctcc ggccgcaccg tggcttccac gcggtgcagt
      481 cgcaccaaac cgaagcagtg gtccacaccc agcgcgagcg cggcggtcgc gactccgccg
      541 ccattgaccg ccttggccac ccagtatccg atccaggcgg accgcagcgc gccgtgtgtg
      601 acgttgccga tcgtcagctg tccgcagaac tgtccgtcca actcgattgt gtagggcagc
      661 atccggcctt tgcgggcctc cgaacgcagc cccgaacaca ccgagggcca cgacatgatc
      721 gcgtgccgcg tcgcccagtc caacccggtg gccggttccc accgctcgag gtggtcgcgc
      781 tcggccagcc gcagccggct ccactgcgcg ccgtcacgca gccgaacggg tcgcaaccgc
      841 accacgccgg ctgacacccg cagcggaccg gcaggctgcg gccatcccgg atgcagggaa
      901 ctcgaccgcc acaggttcac gtcgacacgc cggggcggat cagccgcgtt gagccaggaa
      961 tgcgacgtcg acaatctcgc cggtgcggac ctgttgggcc tcggtgggca cgacgacaag
     1021 acagttcgcc tcggccagcg tcgccagcag gtgcgacgag cccggcgccc cgccgagcgc
     1081 ctgcacgaga tactcgccgg tgtcctgatc ccgcatcagc tgtccgcgca gatacccctt
     1141 gcggccagcc accgactcga tcggcgacag ggcgcgggcc tgcacgattc gccgatgcgc
     1201 ctggcgcttg cccaacgaca gccggatcag cggacgcacc atcacctcga acaccaccag
     1261 tgcgctgacc gggttggcgg gcagcaggaa gaccggcaca ccgtcgcggc cgagctggcc
     1321 gaacccttgc acggaaccgg ggtgcatcgc gatgcgcgtc acccccatct cgcccaagtc
     1381 ggcgagcacc gagcggaccg cctcggcggc cgccccgccg acggcaccgg cgatcaccac
     1441 cacctcggcc cggttgatct gcccctcgac gacctcacgc agccgcttgg ggtcggtgtc
     1501 gacgatgccg acccggttca cctccgcgcc ggcatcacgg c
//
LOCUS       DATADN010000132         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_617885, whole genome shotgun sequence.
ACCESSION   DATADN010000132 DATADN010000000
VERSION     DATADN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617885"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VHH53_01730"
     CDS             complement(<1..1712)
                     /locus_tag="VHH53_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX2288933.1"
                     /translation="MYRTGDRVRWTVEGQLEYLGRTNHQVKIRGYRIELGEIEAALLR
                     RPEVAEAVTVVRQEDSGHKRLVAYVVPVVGGVLDSVELRAYLATMLPDYMVPAAFVAL
                     DGLPLTPNGKLDRRALPAPDFAGVVGGGYIAPRSEAERALADIWADVLGVEQVGVEDN
                     FFELGGDSILSIQVVSRARRAGLGLMPLDVFMHPTVASLVLGVAGVAPVVAEQGPVSG
                     VVALTPIQHWLFETNPVCPQRFDQSVSVELTQGTDERALRRAFDAVLEHHDALRMRFE
                     CVDGRWRQENMPVGPVDVLQRRDLSGIDDPDAQTAAMEQVAGQVRAGFDLSDGPLVRA
                     VLFDLGAQQRPVLFVAVHHLVVDGVSWRILLEDLDTAYAQATRGQTVGLGSKTTSFQN
                     WAQRLTEHASTGGFDAELDYWTQITQGGDPVVPTDGTGANTVASMSAVTVRLDAAQTK
                     ALLHDVPGVYRTQVNDVLLAALGRVLSRWTAQRRVLIDLEGHGREEIFDGVDLSRTVG
                     WFTTMFPLALDIPTTADLGGLLKSVKEQLRAVPGRGLGYGALRYLTPTQALADQPHPQ
                     VSFN"
BASE COUNT          416 a          731 c          462 g          264 t
ORIGIN      
        1 tagttgaagc tgacctgcgg atgaggctgg tcggctagcg cttgggtggg ggtcagatac
       61 cgcagcgctc cgtatcccag gccgcgcccg ggcaccgcgc gcagctgttc tttgaccgac
      121 ttgagcaacc cacccagatc agcagtggtg gggatgtcta atgccagcgg gaacatggtg
      181 gtgaaccagc ccaccgtgcg cgacaggtcc accccgtcga agatctcttc gcggccgtgg
      241 ccttccaggt cgatcagcac ccggcgttgt gcggtccagc ggctgagtac ccgacccaac
      301 gcggccaaca acacgtcgtt gacctgggtg cggtagaccc ccggcacgtc atgcagcagc
      361 gctttggtct gggccgcatc cagccgcacc gtgaccgcgc tcatcgaggc caccgtgttg
      421 gccccggtcc catcggtggg cactaccgga tcaccaccct gggtgatctg ggtccagtag
      481 tccagctcgg cgtcgaaccc gccggtgctg gcgtgctcgg tcaaccgctg cgcccagttc
      541 tggaacgacg tggtcttaga ccccaacccg acagtctggc cgcgggtggc ttgcgcatag
      601 gcggtgtcca aatcctccag caggatccgc cacgacaccc catcaaccac caaatggtgc
      661 accgccacaa acaacaccgg ccgctgctgg gcacccaaat cgaacaacac cgcccgcacc
      721 agcgggccat cacttaaatc gaaacccgca cggacctgcc cggcgacctg ctccatcgcc
      781 gcggtctggg cgtcagggtc atcaatcccg gacaggtcac gccgctgcag cacgtccacc
      841 gggcccaccg gcatattctc ttggcgccac cggccatcca cacattcaaa ccgcatccgc
      901 aacgcgtcat gatgctccag caccgcatca aacgcccgcc gcaaagcccg ctcatcggtc
      961 ccctgggtca actccaccga caccgactgg tcaaaacgct gtgggcacac cggattcgtc
     1021 tcaaacaacc aatgctggat cggcgtcaac gccaccacac cactgaccgg gccctgctcg
     1081 gccaccaccg gcgccacccc ggccacaccc aacaccaacg acgccaccgt cggatgcata
     1141 aacacatcca aaggcatcaa acccaaccca gcccgcctag cccgcgacac cacctgaata
     1201 ctcagaatcg agtcgccacc gagctcgaag aaattatctt caaccccgac ttgttccaca
     1261 cccagcacat cagcccaaat atcggccaat gcccgctcag cctcactgcg cggagcgatg
     1321 taaccaccac ccaccacccc ggcgaagtcc ggcgccggca atgcacgccg gtccagtttc
     1381 ccgttcggcg tcaacggcaa cccatccaat gccacaaacg ccgccggcac catgtaatcc
     1441 ggcaacatgg tcgccaaata tgcccgcaac tcgaccgaat ccaacacccc accaaccacc
     1501 ggaaccacat aagccaccaa ccgcttgtga ccggaatcct cctgccggac aaccgtgacc
     1561 gcctcagcca cctcgggtcg ccgcagcagg gcagcctcga tctcacccag ctcgatccga
     1621 tacccacgaa tcttcacctg gtgattggtc cgacccagat attccaactg cccctcgacg
     1681 gtccagcgca cccggtcccc ggtgcgatac atccgcgaac ccgccgaccc aaacggacac
     1741 gccacaaacc gctgcgcagt caaccccgga cgattcaaat acccacgagc caaccccacc
     1801 ccagcgatga acaactcacc cggaacaccc accggaaccg gacgcagatc agcacccaac
     1861 acataaaccc ggg
//
LOCUS       DATADN010000133         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_316371, whole genome shotgun sequence.
ACCESSION   DATADN010000133 DATADN010000000
VERSION     DATADN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316371"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1572)
                     /locus_tag="VHH53_01735"
     CDS             complement(<1..>1572)
                     /locus_tag="VHH53_01735"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX2288934.1"
                     /translation="PDFTTPLRGAAARVVANMETSLELPTATSVRAVPAKLLVDNRIV
                     INNHLKRTRGGKISFTHLIGYAVVRALADFPQMNRHYISDVHGKPAVVAPEHVNLGLA
                     IDLHTDRGRNLVVASIKRCEAMTFAQFWQAYEDIIRKAREGKLTAEDLADTTISLTNP
                     GTIGTNHSVPRLMSGQGAIIGVGAMEYPAAFQGASEQALADMAVSKIITLTSTYDHRI
                     IQGAESGEFLRRMHGLLLGEDGFYDDIFVSLRIPYEPVRWVQDIPEGEVHKTARVLEL
                     IDAYRTRGHLMADTDPLNYRQRKHPDLDVLSHGLTLWDLDREFAVGGFAGQSNMKLRD
                     VLGVLRNAYCRNIGVEYMHILAPDERQWLQERIERVHDQPSPAEQKYILSKLNAAESF
                     ETFLQTKYVGQKRFSLEGAETVIPLLDAVLDKAAEHELDEVVLGMPHRGRLNVLANVV
                     GKPYSQIFREFEGNLDPGQAHGSGDVKYHLGAEGKYYRMFGEGETKVTLTSNPSHLEA
                     VDPVLEGITRAKQDIL"
BASE COUNT          241 a          486 c          539 g          306 t
ORIGIN      
        1 caggatgtcc tgcttggctc gcgtgatgcc ctccagcacc gggtccacgg cctccaggtg
       61 cgacgggttg gacgtcagtg tcaccttcgt ctcgccctca ccgaacatcc ggtagtactt
      121 gccctcagcg ccgaggtggt acttgacgtc accggagccg tgcgcctggc cggggtcgag
      181 gtttccctcg aactcgcgga agatctgcga gtacggctta cccacgacat tggccagcac
      241 gttgagccgg ccgcggtgcg gcatgccgag caccacctcg tccagttcgt gctcggcggc
      301 cttgtcgagc acggcgtcca gcagcgggat gacggtctcg gcaccctcaa gcgagaaccg
      361 cttctggccg acgtacttgg tctgcaagaa ggtctcgaag gactcggcgg cgttgagttt
      421 ggacaggatg tacttctgtt cggcagggct gggttggtcg tgcacccgct ctatgcgctc
      481 ctgcaaccat tgccgctcgt cgggcgccag aatgtgcatg tactccacgc cgatgttgcg
      541 gcagtaggcg tttcgcagca cgcccaggac gtcgcgcagc ttcatgttcg actgcccggc
      601 gaagccacct acggcgaact cgcggtccag gtcccacagg gtcagaccgt ggctgagcac
      661 gtcgagatcg gggtgtttgc gctgccggta gttcagcggg tcggtgtcgg ccatcagatg
      721 gccacgagtg cggtaggcgt cgatgagctc gagcacccgg gcggtcttgt gaacttcgcc
      781 ctcggggatg tcctgtaccc agcgcaccgg ctcgtagggg atccgcaacg agacgaagat
      841 gtcgtcgtag aagccgtcct cgcccagcag caggccgtgc atccggcgca ggaattctcc
      901 ggactcggcg ccctgaatga tgcggtggtc gtaggtggac gtcagcgtga tgatcttgct
      961 gacggccatg tcggcgagtg cttgctcact ggcgccctgg aaggccgccg ggtactccat
     1021 cgcgccaacg ccgatgatgg caccctgccc ggacatcagc cggggtaccg agtggttggt
     1081 gccgatggtg cccggattgg tcagcgagat cgtggtgtcc gcgaggtcct cggcggtgag
     1141 cttgccctcg cgcgccttgc ggatgatgtc ttcgtaggcc tgccagaatt gggcgaaggt
     1201 catcgcttcg cagcgcttga tcgaggcgac gacgaggttg cggccccggt cggtgtgcag
     1261 gtcgatcgcc aggcccagat tgacgtgctc cggcgccacg acagcgggtt tgccatggac
     1321 gtcactgatg tagtgccggt tcatctgcgg gaagtcggcc agcgcgcgca cgaccgcgta
     1381 cccgatcagg tgggtgaagc tgatcttccc gccgcgggtc cgcttgagat ggttgttgat
     1441 cacaatgcgg ttgtcgacga gcagcttcgc gggcactgcg cgcacgctgg tggccgtcgg
     1501 cagctccagc gaggtttcca tgttcgccac cacacgggcc gctgcacctc gaagcggggt
     1561 ggtgaagtcc gg
//
LOCUS       DATADN010000134         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_14763, whole genome shotgun sequence.
ACCESSION   DATADN010000134 DATADN010000000
VERSION     DATADN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14763"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VHH53_01740"
     CDS             <1..192
                     /locus_tag="VHH53_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2288935.1"
                     /translation="TVVITRGGRQIALIVPAPRANGSAVVEVLDRWRDQLEVDDQFAA
                     NVASAGNESAVVNGDPWRE"
     gene            180..581
                     /locus_tag="VHH53_01745"
     CDS             180..581
                     /locus_tag="VHH53_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2288936.1"
                     /translation="MARVILDTGVLVAAGRGRLNLAAFAEDDVALPAVTLAEYLTGVE
                     LDDNPARRAAHHAFLEELLAVTPVADYTPQVAVHHAALLAQVRRSGCPHGAHDLIIAA
                     TARATDRVLVSTDVQAGFVDLPEVQTRLLAQ"
     gene            594..1205
                     /locus_tag="VHH53_01750"
     CDS             594..1205
                     /locus_tag="VHH53_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX2288937.1"
                     /translation="MTGYPPEPWELRGQQYLTLWRVPSAHLPRLSAGLKPVRAGGTAL
                     VVTSWVIYEPGGMLSYRELFVAVLAHRGGWPLVTITDIWVDSIPSLHGGRELWGIPKQ
                     RASFHVHGTTCAAVNVDGELIASSAVRPVGALPGRCRGRCTVAQQRAGELMFIPVRCI
                     SNVRLIQISWEFAPGGPLGWLADARPAFSASLRDFTLCFGRRH"
     gene            1477..>2104
                     /locus_tag="VHH53_01755"
     CDS             1477..>2104
                     /locus_tag="VHH53_01755"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX2288938.1"
                     /translation="MASTATRPRGEGQWKLGHREPLNRNEQIKKDDDGLNVRARIETI
                     YAHRGFDSIDPADLRGRMRWWGLYTQRKPGIEGGRTGALEPEELEDRYFMMRVRIDGG
                     ALTTEQLRVLGEISRSYARDTADITDRQNIQYHWIQIEDVPTIWQQLEAVGLSTTEAC
                     GDCPRVVLGSPVAGICADEVTDGTPAIEEINRRYLGDPAFSNLPRKYKT"
BASE COUNT          318 a          708 c          721 g          357 t
ORIGIN      
        1 acagtggtga tcacgcgcgg tggacggcag atcgcactga tcgtgccagc accgcgggct
       61 aatggcagtg ccgtggtgga ggtgctggat cgttggcggg atcagctgga ggtcgacgac
      121 cagttcgcag cgaacgtggc ctcggctggc aacgagtcgg cggtcgtgaa cggcgatccg
      181 tggcgcgagt gatccttgac accggggtgt tggttgccgc gggtcggggt cggctcaacc
      241 tcgcggcgtt cgcggaggac gatgtggcgc ttcctgcagt gacgctggct gagtacctga
      301 ccggtgtcga gcttgatgac aatcccgctc gtcgtgccgc gcaccacgca ttcctcgagg
      361 agctgttggc tgtgactccc gtggctgact acaccccgca ggtggcagtg caccacgccg
      421 cattgttagc ccaggttcgc cgctccgggt gtccccacgg cgctcacgac ctgatcatcg
      481 cggccaccgc gcgcgcgacc gaccgggtct tggtctccac tgacgtgcag gccggattcg
      541 tcgaccttcc cgaggtgcag acccggctgc tggctcagtg acaggatggc tcagtgaccg
      601 ggtacccacc ggagccctgg gagctgcggg gtcagcagta cctcacgcta tggcgggtgc
      661 cgtcggcgca cctgccccgg ctcagcgcag ggctcaagcc ggtgcgagcc ggtggcaccg
      721 cgctggtcgt cacctcgtgg gtgatctacg aaccgggtgg gatgctgtcc taccgtgagc
      781 tgttcgtggc cgtcctggcg caccgaggcg ggtggccgct ggtgacgatc acggacatct
      841 gggtggacag cattccttcg ttgcacggtg ggcgcgagct gtggggaatc cccaagcagc
      901 gggcgtcctt ccacgtgcac ggcaccacct gcgcagcggt gaacgttgac ggcgagctga
      961 tcgccagctc cgccgtccgc ccggtcggtg cgctccctgg gcgctgccgc ggccggtgca
     1021 cggtggcgca gcagcgcgcc ggcgagctga tgttcatccc ggtccgctgc atctccaacg
     1081 tgcggctgat ccagatcagc tgggagttcg cgcccggtgg tccgctcgga tggcttgccg
     1141 acgcccgccc ggccttctcc gcctcactgc gggacttcac gctgtgcttt gggcggcgcc
     1201 actgataccg atccgtgtct caccatgcgg gcggtggtgc aatttggcat ttcgctgtgg
     1261 cacgctcgtc acgtgaccgc cgtcgggatc ccgctcgtgg cgcgtcgcca cgtcgatttc
     1321 cggcgagtct ccagcgcgat ctgtcgaccg gcggactaac cccgccgccg ctgcgctttt
     1381 ctcctcatcc ccagccggcg ccgggcgcgc tcgctggagg tcggcgacct caggtaatga
     1441 gcgcgcgcct gcgtccccag cctgaaggtc tcaatcatgg cctcgacagc gactcgtccc
     1501 cgcggtgagg gccagtggaa actgggccac cgcgaaccac tcaaccgcaa cgagcagatc
     1561 aaaaaagacg acgacggcct gaacgtccgg gcccgcatcg agacgatcta cgcacaccgg
     1621 ggcttcgact cgatcgatcc tgccgacctg cgcggcagga tgcgctggtg gggcctttac
     1681 acccagcgca agccgggcat cgagggcggc cgcaccggcg cgttggagcc agaggagctc
     1741 gaggaccgct acttcatgat gcgggtccgg atcgacggcg gcgccctgac taccgagcag
     1801 ctgcgggtgc tcggtgagat atcgcgaagt tatgcccgcg acaccgccga catcaccgac
     1861 cggcagaaca tccagtacca ctggatccag atcgaggacg tgccaacgat ctggcagcag
     1921 ctggaggccg tcgggctgtc caccaccgag gcctgtggcg actgcccccg ggtggtcctg
     1981 ggttcgccgg tcgccggcat ctgcgccgac gaggtgaccg acggcacgcc ggcgatcgag
     2041 gagatcaacc gccgctatct cggcgatccg gcgttctcca acctgccacg caagtacaag
     2101 acgg
//
LOCUS       DATADN010000135         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_919551, whole genome shotgun sequence.
ACCESSION   DATADN010000135 DATADN010000000
VERSION     DATADN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919551"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VHH53_01760"
     CDS             <1..436
                     /locus_tag="VHH53_01760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2288939.1"
                     /translation="EYALHRPDYPDQAVAWALASMLSSGPNGGPVLDGRVLDLGAGTG
                     KLTTAILRCGMATTQVLAVEPDDGMRAELRRRLPEITILNGSAEQIPAEEGAVDVVLV
                     GQAFHWFDLDRALSETARVLRPGACWRRWVIPRMTPSAGWPS"
     gene            493..738
                     /locus_tag="VHH53_01765"
     CDS             493..738
                     /locus_tag="VHH53_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288940.1"
                     /translation="MPQHPAFGEPEQREFCWRWPRTIDSFLATVSTHSWALVSTPEQR
                     AAALAAIRRFLEQHPATRDGAFDVPMRTLVQRMVRHA"
     gene            complement(839..1636)
                     /locus_tag="VHH53_01770"
     CDS             complement(839..1636)
                     /locus_tag="VHH53_01770"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX2288941.1"
                     /translation="MLLALDIGNTNIALGLFGGDQGGTRAGAMLVRDWRMHTDARMTA
                     DELALTVRGLLGEYSGKVTGIAALSTVPALLRELRVMLDRYWSPLPRVIVEPGVRTGV
                     PLHYDNPREVGADRIVNTLAAYTLYGGPAIVVDFGTSTNFDVISARGEFLGGALAPGI
                     EISVDALAARAAQLVKVQLVRPRSVIGKSTVEALQSGLLFGFAGQVDGLVGRITAELA
                     PAANSEVVVVATGGLAPLLIAESKTITRHAPDLTLQGLRMVYERNTS"
BASE COUNT          286 a          560 c          558 g          258 t
ORIGIN      
        1 tgagtatgca ctgcaccggc cggattaccc ggatcaggcg gtggcatggg cgctggcctc
       61 aatgctcagt agtggaccca acggtggccc agtactcgac ggcagagtgc tcgacttggg
      121 cgcgggcact ggcaagctca ccacggcgat actgcggtgt ggaatggcca cgactcaggt
      181 gctcgccgtt gagccggacg acgggatgcg tgcggagctg cgccgccggc tacccgagat
      241 aacgatcctc aacgggtctg ctgagcagat cccggccgaa gagggtgccg tggatgtggt
      301 gctcgtcggg caggccttcc attggttcga cctcgaccgg gcgctgtcgg agactgcccg
      361 ggtactacgg ccgggggcgt gctggcggcg atgggtaata ccgaggatga ctccgtcggc
      421 tgggtggccg agctgagcga gacggcccaa gctgcatggc cgatcggtag ccccgggcga
      481 ggattcgccg atgtgccgca acacccggcg ttcggcgagc cggagcagcg tgaattctgc
      541 tggcgctggc cgcgcaccat cgactccttc ctcgcgaccg tgtcaactca ctcatgggcg
      601 ctggtcagta cacccgagca acgagccgcc gcgctggccg caatccgccg tttcctcgaa
      661 cagcaccccg ccacccgtga tggtgccttc gacgtaccga tgcggacgct ggtgcagcgc
      721 atggtccgtc acgcctgaca caccgggcat ccgcgcctga cataccgggt atcagagcgc
      781 gacacaccgg gcagggggac ggacacgccg gggatggggg tgaggacggg gtaagggatc
      841 agctggtgtt gcgttcgtag accatccgca atccctgcag agtgagatcg ggcgcgtggc
      901 gcgtgatggt cttcgactcg gcgatcagca gcggcgccag ccccccggtg gcgaccacca
      961 ccacctcgct gttggctgcc ggtgccagct cagcggtgat ccggccgacc agcccgtcga
     1021 cctgcccggc aaacccgaat agcagcccgg actgcagggc ttccacggtg ctcttgccga
     1081 tcaccgatcg cggccggacc agctgtacct tcaccagctg agcggcccgc gcggcgagcg
     1141 catcgacgga gatctcgata ccaggagcca gcgcgccgcc gaggaactcc ccgcgtgcgc
     1201 tgatcacgtc gaagttggtg gaggtaccga agtccaccac gatcgccgga ccaccgtaga
     1261 gggtgtaggc ggccaacgtg ttgacgatcc gatcggcgcc cacctcccgc gggttgtcat
     1321 agtgcaacgg caccccggta cgcaccccgg gctccacgat cacccggggc agtggggacc
     1381 agtaccggtc cagcatcacc cgtagttcgc gtaacagagc aggcacagta gacagtgccg
     1441 cgatcccggt caccttgccg gagtactcgc cgagcagccc gcggacggtc agcgccagct
     1501 catcggcagt catcctggcg tcggtatgca ttcgccagtc gcgcaccagc atggcgcccg
     1561 ccctcgtgcc accctgatca ccgccgaaca acccgagggc gatgttggtg ttgccgatgt
     1621 ctagggcgag cagcactcgc tacccctgac gagattcgcc cc
//
LOCUS       DATADN010000136         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_316542, whole genome shotgun sequence.
ACCESSION   DATADN010000136 DATADN010000000
VERSION     DATADN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316542"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VHH53_01775"
     CDS             <1..230
                     /locus_tag="VHH53_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288942.1"
                     /translation="AAPPPGPSPCPSGGRCPQPAEAAPASFGGNVGPVGSQQERNQLS
                     VITGQPATTATQFLLGPVARGTTVSLNEEAR"
     gene            227..1282
                     /locus_tag="VHH53_01780"
     CDS             227..1282
                     /locus_tag="VHH53_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2288943.1"
                     /translation="MRYRGALIGLSLFMVVAVTLTWLVYVTLRRDVAGRTVPYAAMIT
                     DVFGLREGDDVRMAGVRVGRVEKIDLQGNLAKVSFVVQADQQVLGTTVASVTYQNIVG
                     QRYLGLSLGNLGDPGPLEPGSVIPVQRTDPSFDVGRLLNGYEPLFSVLDPKHADNLTK
                     GVIQSLQGDEGSITALVDQTSQLTESFAGQDEQLGEVITDLNVVVANLARHNDDLDHI
                     IGEARTVVSTFDARRPELVEAMSSIAKVVRQLSTISDEVYPSLNELVTREPGFAAHMV
                     GLENQLAFTGANLPLLLKGFARITSEGSFANAYACDLNIQGFFPGLNDVVPIIVDAAT
                     PGNKARYTPKCRNMANG"
     gene            1275..>1591
                     /locus_tag="VHH53_01785"
     CDS             1275..>1591
                     /locus_tag="VHH53_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2288944.1"
                     /translation="MADQATAWRRRRPLENRNKTWMGIVAVLVTALLVGTLLLVKVAN
                     FGYRQYTAHFLQAAALQVGNPVTVAGIPVGEVMSLDLAGDHVEANLKVRNDVVLGQDS
                     RAS"
BASE COUNT          276 a          534 c          512 g          269 t
ORIGIN      
        1 cggctgcgcc gccgcccggt ccgtccccct gcccttcggg cgggaggtgc ccccagcccg
       61 ccgaagcggc gccggcgtca ttcggcggca atgtgggacc cgttggcagc caacaggaac
      121 gcaaccagct ctccgtcatc acgggccagc cggccaccac ggcgacccag tttctgctcg
      181 gcccggtggc ccgcggcacg accgtctcgc taaatgagga ggcccgatga ggtaccgcgg
      241 cgcgctgatt ggcctgtcgc tgttcatggt ggttgcagtg acgctgacct ggctggtgta
      301 cgtcaccttg cgtcgagacg tggccggccg gacggtgccc tacgccgcga tgatcaccga
      361 cgtgttcggc ctgcgggagg gcgatgacgt ccggatggcc ggcgtccgcg tcggccgggt
      421 cgaaaagatt gatctgcagg gaaacctggc caaggtgtcg ttcgtggtgc aggccgacca
      481 gcaggtgctc ggcaccaccg tggcatcggt cacttaccag aacatcgtcg gccagcgata
      541 cctcggtctg tcgctgggca atctcggtga cccgggaccg ctggagcccg gcagcgtcat
      601 ccccgtccag cgcaccgatc cgtcgttcga tgtcggccgt ctgctcaacg gttacgagcc
      661 gctgttcagc gtgctcgatc ccaagcacgc cgacaacctc accaagggag tcatccaatc
      721 tctgcagggt gacgaagggt ccatcaccgc cctggtggac cagaccagcc agctcaccga
      781 gtcattcgcc gggcaggacg agcaactcgg tgaggtgatc accgacctga acgtggtggt
      841 ggccaacctc gcccggcaca acgacgacct cgaccacatc atcggggaag cccgcacggt
      901 ggtgtcgacc ttcgacgctc ggcgacccga actggtggag gcgatgagct cgatcgccaa
      961 ggtggtgcgt cagctctcga cgatctccga cgaggtgtac ccgtcactga acgaacttgt
     1021 cacccgtgag ccgggattcg cggcgcacat ggtcggtctg gagaaccagc tggcgttcac
     1081 cggcgccaac ctcccgttgc tgctgaaggg cttcgcgcgc atcaccagcg aaggctcctt
     1141 cgccaacgcc tacgcatgtg acctgaacat ccaaggattc ttccccggcc tcaacgacgt
     1201 ggtgccgatc atcgtggacg ccgccacccc gggcaacaag gcgcgctaca ccccgaagtg
     1261 caggaatatg gccaatggct gatcaggcaa cggcgtggcg ccggcgacgc ccgctggaga
     1321 accgcaacaa gacctggatg ggcatcgttg ccgttctggt gactgcgttg ctcgtcggaa
     1381 cgctgcttct ggtcaaggtg gccaacttcg gctaccggca gtacaccgca cacttcctgc
     1441 aggccgccgc gctgcaagtc ggtaaccccg tcacggttgc cggcatcccg gtgggcgagg
     1501 tgatgagctt ggacctcgcc ggtgaccatg tcgaggccaa tctcaaggtg cgcaacgacg
     1561 ttgtgttggg ccaggattcc cgggcatcga t
//
LOCUS       DATADN010000137         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_768814, whole genome shotgun sequence.
ACCESSION   DATADN010000137 DATADN010000000
VERSION     DATADN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768814"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..>1576
                     /locus_tag="VHH53_01790"
     CDS             212..>1576
                     /locus_tag="VHH53_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX2288945.1"
                     /translation="MTTNQTDVESPAVVRGDDAYHLTPEGIKEPPEGWGPSLRYFGPG
                     LILSASIVGSGELIATTTLGAQAGFAILWMVIFSTLVKVAVQVEFARWTISTGQPALT
                     GYNKVPPKIGPLGWVNALWTVMALSKILQIGGIVGGVAIAFSVLMPLGGDPLGFTSTM
                     IWTIIVVVGSIALLYSNRYGLIERGAVLLVVVFSTITVLIALGLPFTPFAYSSGDILG
                     GLTFAIPAGALGAAIAMFGITGVGADEITFNTYWCVEKGYARWVGPNDGSDEWARRAN
                     GWIRVMYKDAFISWLIYTFGTLAFFIMGAAVLQPQGLIPEGNEMITTLSRIYTDTLGS
                     WASILFLIGAIAVLGSTLWAAIPSWSRMCTNLLSTAGVLDWQDAPTRLRWIRGFTVAL
                     PILWGAAYLFIQSPVLMVQIGGVMTGIFLLAVVVAVWYLRNTEVDRRLYGSALFNTVL
                     IISSL"
BASE COUNT          233 a          533 c          508 g          302 t
ORIGIN      
        1 ccgcgccccg gtcgcacagc ctcaagaccg tagccctcga cgggtacccg gtctgccacc
       61 ggcacggcga ggccgatctg gagcccgcct gccacatatt gatacttctg tactctgttg
      121 gcgcgctgtg ctaggctggg aagcctaggg ggtgtggaag ccggctcagg ccccgtgacc
      181 ggtggtcggg cagtctcgaa ggagggcgct cgtgacgacg aaccagaccg acgtagagtc
      241 cccggcggtg gtgcgcgggg acgacgcgta tcatctcacc ccggaaggca tcaaagagcc
      301 tcccgagggt tggggcccga gcctgcggta cttcgggccg ggtttgatcc tgagcgcctc
      361 gatcgtcggt tcgggggagt tgatagccac caccaccctg ggcgcccagg ccgggttcgc
      421 catcctgtgg atggtgatct ttagcaccct cgtcaaggtg gcggtgcagg tggagttcgc
      481 gcgctggacc atctcgaccg gccagccggc gctcaccggc tacaacaagg tgccgcccaa
      541 aatcggcccg ctcgggtggg tcaacgccct gtggaccgtg atggccctct cgaagatcct
      601 tcagatcggc ggcatcgtcg gcggcgtggc gatagccttt agtgtgctga tgccgttggg
      661 cggcgacccg ctcgggttca cctccaccat gatctggacg atcatcgtcg tcgtcggaag
      721 catcgccctg ctgtactcca accgctacgg tctcatcgag cgcggggccg tgctgctggt
      781 ggtggtcttc tcgaccatca ccgttctgat cgccctcggt ctgccgttca cgccgttcgc
      841 ctacagctcg ggcgacatcc tcggcggcct caccttcgcc atcccggccg gggccctggg
      901 cgcggccata gccatgttcg ggatcaccgg cgtcggcgcc gacgagatca ccttcaacac
      961 ctactggtgc gtcgagaagg gctacgcccg gtgggttggc cccaacgacg gcagcgacga
     1021 gtgggcgcgg cgggcgaacg gttggatccg ggttatgtac aaggacgcct tcatctcctg
     1081 gcttatctac accttcggca cgctggcctt cttcatcatg ggcgcggccg tcctccagcc
     1141 gcagggcctc ataccggagg gcaacgagat gatcacgacc ctctcgcgca tctacacgga
     1201 cactttgggc tcgtgggcca gcatcctctt cctgatcggc gccatcgcgg tgctcggctc
     1261 gaccctgtgg gccgcgatac cgagctggtc gcgcatgtgc accaacctcc tgtctaccgc
     1321 gggcgtcctt gactggcaag acgcgccgac ccgcctgcgc tggatccgcg gcttcaccgt
     1381 ggcgctgccg atcctctggg gcgcagccta cctgtttatc cagtcgccgg tgctcatggt
     1441 ccagatcggc ggcgtcatga ccggtatctt cctgctggcc gtggtggtgg ctgtctggta
     1501 cctgcgcaac accgaggtag atcgccgcct ctacggcagc gccctgttca acacggtgct
     1561 gatcataagc agcctc
//
LOCUS       DATADN010000138         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_768979, whole genome shotgun sequence.
ACCESSION   DATADN010000138 DATADN010000000
VERSION     DATADN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768979"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /gene="ftsY"
                     /locus_tag="VHH53_01795"
     CDS             <1..1357
                     /gene="ftsY"
                     /locus_tag="VHH53_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX2288946.1"
                     /translation="SFSSGGGGPPAPPQRPAAPAPPRASAPAPPKPPVQAPPKPPAQA
                     PPKPPAQAPPKPPAQAPPKPPTQAPPKPPAAPVTPQPAAPARPESTAPPASVAPPAPP
                     PTKPAPAKPVPAKSAPVKSAPVKLAPVEPVPAPVEVIEPPSGRLVRLRGRLSRSRSAF
                     GQSLLGLLGAGDLDEDSWTDVEDTLLLADLGTATTTEIVDRLRAELAQRGVRTAEQAR
                     AALRDVLIEALRPELDRAVRALPHQDGSNGASVPGVVLIAGVNGTGKTTTTGKLARVL
                     VADGRRVLLGAADTFRAAAVDQLATWGQRAGAEVVRGPEGADPASVAFEAVKRGSADG
                     VDTVLIDTAGRLHTKTGLMDELGKVKRVVSKQAEVDEVLLVLDATTGQNGLIQARVFR
                     EVVDVTGIVLTKLDGTAKGGIVFHVQRELGVPVKLVGLGEGVDDLAPFEPAAFVDALL
                     D"
     gene            1525..>1693
                     /locus_tag="VHH53_01800"
     CDS             1525..>1693
                     /locus_tag="VHH53_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX2288947.1"
                     /translation="MLDTGDTAWMLISSALVLLMTPGLAFFYGGMVRSKSVLNMMMMV
                     LGALAVVTVVWV"
BASE COUNT          281 a          584 c          554 g          274 t
ORIGIN      
        1 cagcttctcg tccggcggcg gtggtccacc ggcccccccg cagcggccgg ccgccccagc
       61 tccgccccgg gcctcagcgc cggcaccacc gaaaccgccc gtgcaggcgc cacctaaacc
      121 gcctgcccag gcaccaccca aaccgcctgc ccaggcacca cccaaaccgc ctgcccaggc
      181 accccctaag ccgcccacac aggcaccacc gaaaccgcca gctgcaccgg tcacgcccca
      241 acctgcagcg ccggcacggc cagaaagcac ggctccaccg gcgagtgtcg cgccgccagc
      301 gccgccgccc acgaagccgg cgcctgccaa accggtgcct gccaaatcag cgcccgtgaa
      361 gtcagcgccg gtcaagctgg cgcccgtcga acccgtccct gcgcctgtcg aggttatcga
      421 gccaccttcc gggcggctgg tgcggttgcg cggccggttg tcccggtccc gctcggcgtt
      481 cgggcagagc ctcctgggcc tgctcggtgc aggtgatctg gatgaagatt cctggactga
      541 tgtcgaggac accttgctgc tcgccgatct cggcacggcc accaccaccg agatcgtcga
      601 ccggctgcgt gctgaactcg ctcagcgcgg tgtccgtacc gccgagcagg cccgtgccgc
      661 gctgcgagat gtgttgatcg aagcgctgcg tcctgagctg gaccgtgccg tgcgggcgtt
      721 gccacatcag gacggcagca acggcgccag cgttcccggc gtggtgctga tcgcaggagt
      781 aaacggcacc ggcaagacga ccaccaccgg caagctggcc cgcgtgctgg tggccgacgg
      841 ccgccgggtg ttgctcggcg cagccgacac tttccgggca gccgccgtgg accagttggc
      901 cacctggggg cagcgcgctg gcgcggaagt tgtgcgggga cctgagggtg ccgacccggc
      961 cagcgtggca ttcgaggcgg tcaagcgggg cagcgccgat ggggtggaca ccgttttgat
     1021 cgacactgcc ggccggctgc ataccaaaac cggtctgatg gatgagctgg gcaaggtcaa
     1081 gcgcgtggtg tccaagcagg ccgaagtgga tgaggttctg ctggtgctgg acgccaccac
     1141 gggccagaac ggtctgatcc aggcgagggt tttccgtgag gtcgtcgatg tcacgggcat
     1201 cgtgttgacc aagttggacg gcactgccaa gggcggcatc gtcttccacg tgcagcgcga
     1261 gctcggtgtg ccggtcaagc tagttggtct tggcgagggc gtcgatgatc ttgctccatt
     1321 cgagccagcg gcctttgtcg acgcgttact ggactgatcg cgccaaacgg ccgctaccac
     1381 gcgcgtgtag ccgatctgaa tctggacgta acagtacaag gccgacagtt aacgtcggcg
     1441 aaacataccg tgtccttggt cgaaacaccg actgctcaac ctgtcctgca acgccacccg
     1501 atcgtgagat gcagaggagt gaccgtgtta gacaccggtg acaccgcctg gatgctcatc
     1561 agctccgccc tggtgctgct gatgactcca ggtttggcgt tcttctacgg cggcatggtc
     1621 cgctccaaga gcgtcctcaa catgatgatg atggtgctcg gagcgctcgc cgtcgtgacc
     1681 gtcgtctggg tgc
//
LOCUS       DATADN010000139         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_316825, whole genome shotgun sequence.
ACCESSION   DATADN010000139 DATADN010000000
VERSION     DATADN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316825"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..302)
                     /locus_tag="VHH53_01805"
                     /pseudo
     CDS             complement(54..302)
                     /locus_tag="VHH53_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
     gene            302..478
                     /locus_tag="VHH53_01810"
                     /pseudo
     CDS             302..478
                     /locus_tag="VHH53_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate transport system regulatory protein
                     PhoU"
     gene            complement(485..739)
                     /locus_tag="VHH53_01815"
     CDS             complement(485..739)
                     /locus_tag="VHH53_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288948.1"
                     /translation="MGVPLTDAYPKPLTDEVVQASDVVITMGCGDACPIYPGKRYLAW
                     DLDDPAGQPVEQVRAIRDDIDRRVRELLAELAPTPAAGMG"
     gene            complement(817..960)
                     /locus_tag="VHH53_01820"
     CDS             complement(817..960)
                     /locus_tag="VHH53_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288949.1"
                     /translation="MQGGPAVDAGPVPASVDRAGDGGCLLAGRSLPGLGATLNAVQVD
                     ASG"
     gene            complement(987..1343)
                     /locus_tag="VHH53_01825"
     CDS             complement(987..1343)
                     /locus_tag="VHH53_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2288950.1"
                     /translation="MSKPVLPLLCCSPRTGEPLCAEEAAELARVFKAIADPVRLRLLS
                     LIASHAGGEACVCDLTGAFALTGPTISHHLKVLREAGLVDSERRGTWIYYRVQPDLLA
                     ELSAVLVRDVAAKASA"
     gene            1423..>1650
                     /locus_tag="VHH53_01830"
     CDS             1423..>1650
                     /locus_tag="VHH53_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2288951.1"
                     /translation="MSRMQLALRVSDLEGSVTFYSTLFGVKPAKRRPGYANFAITEPP
                     LKLVLLQGETGQPTVMDHLGVEVESTEQVTAA"
BASE COUNT          293 a          560 c          531 g          266 t
ORIGIN      
        1 agctgctcgc cgaactggct ccatgccggc acccgggcat ggactgatcc gactcagacc
       61 accagtgggc cgggcatccg gccggtcacc gtatagatca cgcggcgggc caccgccacc
      121 gcgtggtcgg cataacgttc gtagaagcgg gccagcaacg tcacgtctac cgccgcggct
      181 accccgtgcg gccagtcgcg atccatcagc acggtgaaca tgtgccggtg cagatcatcc
      241 atcgcgtcat cgtcggcctc cagctcgcta gcctgttcca ggtcccgaga gcggatcacc
      301 tcaccggcac atgttcaccg tgctgatgga tcacgactgg ccgcacgggg tagccgcggc
      361 ggtggacgtg acgttgttgg cccgcttcta tgaacgttat gccgaccacg cggtggcagt
      421 ggcacgccgc gtgatctata cggtgaccgg ccggatgccc ggcccgctgg tggtctgagt
      481 cggatcagcc catgcccgct gccggcgtgg gagccagttc ggcgagcagc tcgcggacgc
      541 ggcggtcgat gtcatcgcgg atagcgcgga cctgctcgac gggctgtccg gcgggatcgt
      601 cgaggtccca ggccagatac cgcttaccgg ggtagatcgg gcacgcgtca ccgcagccca
      661 tagtgatcac aacatcggag gcctgaacga cctcatcggt cagcggcttg gggtaagcgt
      721 cggtcagggg cacgccgatt tcccgggcag agccgtacgg cgaacttttt ggtcaggtcg
      781 tgggagatgg cgcgcaggcc gtgctcggtt tcgagttcac ccagatgcgt cgacctgcac
      841 cgcgttcagc gtcgcgccga gtccgggcag cgaccggccg gcgagcaggc aaccgccatc
      901 gccagcccga tccacactgg caggaaccgg tccagcgtcg acagccggcc caccctgcac
      961 ctcctgctcc gaagtgctga tcgcggttat gcactggcct tcgcggccac gtcgcggacc
     1021 aggaccgcgg acagctccgc cagcaggtcg ggttgcaccc gatagtagat ccacgtgccg
     1081 cgacgttccg agtccactag cccggcctcg cgaagcacct tgaggtgatg cgagatcgtc
     1141 ggtccggtca gcgcaaacgc cccggtcagg tcacagacgc aggcttcccc gccggcgtgc
     1201 gacgcgatga gcgagagcag tcgcaaccgg accgggtcgg cgatcgcctt gaacacccgc
     1261 gccagctcgg cggcctcctc agcgcatagg ggctcgccgg tccgcggcga gcagcacagc
     1321 agcggcagaa ctggcttcga catacgtcta tcttgacatg catctacttc aagcgcactc
     1381 tagaggacat cggttagaca gatatctaaa caaggagagg tcatgtcacg gatgcaactg
     1441 gcactgcggg tttccgacct cgaaggctcg gtcaccttct actcgacgct gttcggcgtc
     1501 aaaccggcca agcgccggcc gggttacgcc aacttcgcca tcaccgagcc accactgaag
     1561 ctggtgctcc tccaaggcga gacgggtcaa cctacggtga tggaccacct cggcgtcgag
     1621 gtcgagtcca ccgagcaggt caccgccgcg
//
LOCUS       DATADN010000140         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_165906, whole genome shotgun sequence.
ACCESSION   DATADN010000140 DATADN010000000
VERSION     DATADN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165906"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VHH53_01835"
     CDS             complement(<1..569)
                     /locus_tag="VHH53_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX2288952.1"
                     /translation="MTSAYPVTAVLRNTWRGLTSMRTALVLLFLLALAALPGALLPQY
                     SLNPERVRTYKAQYPTLAPILDRLGFFEVFASPWFAAIYLLLFVSLVGCVLPRSVEYA
                     RQLRRAPVATPRNLARLPHPARGESDALPPEVLAAARRRLRGWRIAEREEPGGARTLS
                     AERGYAREAGNLVFHVALLGLLVSFAVGKL"
     gene            complement(570..1325)
                     /locus_tag="VHH53_01840"
     CDS             complement(570..1325)
                     /locus_tag="VHH53_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX2288953.1"
                     /translation="MGLTELATSGPLLLAAGVAVLAGAVSFASPCVVPLVPGYLAYLT
                     GLVGAESGAQSRGRVLGAVALFVFGFSVVFTASVVTVLGIADRLVLNEITLQRVGGVI
                     TIAMGLVFVGLIPTLQRDLRVHPRPGLGPRHMLGAPLLGAVFGLGWTPCLGPTLVGVI
                     ALAVGTPGGGLARGVLLVAAYCLGLGLPFLLLALGARRAMRAVDWLRKHVRGVQLAGG
                     AMLIAVGLLLATGLWAELIAVLRGPITGFETPI"
BASE COUNT          313 a          587 c          563 g          178 t
ORIGIN      
        1 agcttgccga ccgcgaagga caccagcagc cccagcaacg cgacatgaaa gacgagattg
       61 ccggcctccc gtgcatagcc ccgctcggcc gacagcgtgc gggcaccgcc gggttcctcc
      121 cgctcggcga tccgccaacc gcgcagccgt cgccgggcgg cggccagcac ctcgggtggc
      181 agtgcgtcgg actcgccgcg agcgggatgt ggcagccggg ccaggttacg gggggtggcc
      241 accggagccc ggcgcagctg ccgcgcgtac tcgacgctgc ggggcagcac gcaacccacg
      301 agggatacga acagcagcag gtagatcgcg gcgaaccagg ggctggcgaa gacctcgaag
      361 aagccaagcc ggtccagtat cggagccagc gtcgggtact gggccttgta tgtccggacc
      421 cgttcggggt tcagcgagta ctgcggtagc agcgcgccgg gtagcgcggc cagcgccagc
      481 aggaacagca gcaccagcgc ggtgcgcatg ctggtcagcc cgcgccaggt gttgcgcagc
      541 accgcggtga cggggtaggc cgaggtcatc tagatcggcg tctcgaagcc ggtgatcggt
      601 ccgcgcagga ccgcgatgag ttccgcccac aggccggtgg ccaggagcag cccgaccgcg
      661 atcaacatgg caccgccggc cagctgtacc ccgcggacgt gcttgcgcaa ccagtccacc
      721 gcgcgcatag cgcgccgggc accgagggca agcagcagga agggcagtcc cagcccgagg
      781 cagtaggccg ccaccagcag caccccgcgg gccagaccac cgccgggggt gccgacggcc
      841 agcgcgatca ctcccaccag ggttggcccc aggcacggcg tccaacccaa accgaacacc
      901 gcgcccagca gcggggcgcc gagcatgtgc cggggcccca gtccgggtcg cgggtgcacc
      961 cggaggtcgc gctgcagggt agggatcagc ccgacgaaga ccaaccccat cgcgatggtg
     1021 atcacgccac cgacgcgctg cagagtgatc tcgttgagga caagccgatc cgcgatgccg
     1081 agcacagtga ccacactggc cgtgaacacc accgagaacc cgaacacgaa caacgcaacc
     1141 gcgccgagca cccggcctct gctctgggca ccgctctccg cgccgaccaa cccggtgagg
     1201 taggccaggt accccggcac cagcggaacc acacacggcg aggcgaagct gaccgcgccg
     1261 gcgagcaccg ccaccccggc cgctagcagc agcggcccgg aggtagcgag ttcggtgagg
     1321 cccacgccgg tcactgtagg tcgaggtagc ccttcaagta ccaccaaccc aggtgcgggc
     1381 gctctcaaca accgccaacc cggcgaggaa atgcggccgt ggagcccggt gcaccgactc
     1441 ggcggatagc ggatcgacgt ccgtcgtggt ggccgtggtg caaccgggca aggtgaagtc
     1501 agtcccgctg gatgcctcgc acggcgagga gcaacgatga cacgccgccc acaccctcca
     1561 ctggggtgac gacgaaaccc cggatcggcg gcgccgctat ggtcgactcg atgttggaca
     1621 tctcgcgcat ctccaacatc g
//
LOCUS       DATADN010000141         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_317090, whole genome shotgun sequence.
ACCESSION   DATADN010000141 DATADN010000000
VERSION     DATADN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317090"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VHH53_01845"
     CDS             complement(<1..399)
                     /locus_tag="VHH53_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005449307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX2288954.1"
                     /translation="MTLAQQAGKDKAPDAVPEGHVRLTIDGHEVDAPKGELVIRTCER
                     LGIIVPRFCDHPLLDPAGACRQCLVEAEMGGKPMPKPQTSCTLTVADGMVIKTQHTSA
                     VADKAQQGVMELLLINHPLDCPVCDKGGECP"
     gene            complement(396..1715)
                     /gene="nuoF"
                     /locus_tag="VHH53_01850"
     CDS             complement(396..1715)
                     /gene="nuoF"
                     /locus_tag="VHH53_01850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX2288955.1"
                     /translation="MLTPVLTRRWLSPRSWTLETYTQLEGYTALRTALAAHPDQLIQL
                     IKDSGLRGRGGAGFATGMKWGFIPQAKPGEQPTGPAAKPTYLVINADEGEPGTCKDVP
                     LMMADPHSLIEGCIITSYAVRAHLCFIYVRGEVVHCLRRVHNAVREAYAAGYLGKNIL
                     DSGFDLDIVVHAGAGAYEAGEETALLNSLEGRRAEPRLKPPFPAVSGLYAAPTVVNNV
                     ETICSVPYIVNGGSDWFRTMGTEKSPGPKIYSISGHVQRPGQYEAPLGITLRELLDMA
                     GGMKGGPLKFWTPGGSSTPLLTDEHMDVPLDFEGAAEAGSMLGTTAVMVFNNTVSVPW
                     AVMKWTEFYKHESCGKCTPCREGTYWLTQILQRMVRREGTPSDVETMLDVCDNILGRS
                     FCALGDGAASPITSGIKYFRQEFLDLCTKQPEFERPAQQATLAGSHR"
     gene            complement(1748..>2151)
                     /locus_tag="VHH53_01855"
     CDS             complement(1748..>2151)
                     /locus_tag="VHH53_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2288956.1"
                     /translation="IGHEETAGRPGSKGSITLEHAECLAACDLAPVLQVNYEFYDNQT
                     PESAVELVDALRRGDRPAPTRGAPLTDWRTVELQLAGFFGEPQELRDAVDGPSAAKET
                     LAGNRLAEDRGWQAPAMPDKAPPLPEVADKK"
BASE COUNT          325 a          750 c          695 g          381 t
ORIGIN      
        1 cgggcactcg ccgcccttgt cacagaccgg gcagtccagc gggtgattaa tcagcagcag
       61 ctccatcaca ccctgctgag ccttgtccgc caccgccgac gtgtgctggg tcttgatgac
      121 catgccatcg gccacggtga gagtgcagct tgtctgcggc ttgggcatcg gtttcccacc
      181 catctccgcc tcgaccaggc actgccggca ggcgccggcc gggtcgagca gcgggtggtc
      241 gcagaaccgt ggcacgatga tgcccagtcg ctcgcaggtt cggatcacca gctcgccctt
      301 gggcgcatcg acctcgtggc catcaatcgt cagccggacg tgcccctcag ggacggcgtc
      361 cggagccttg tccttgcctg cctgctgtgc cagcgtcatc ggtgcgatcc tgcgagcgtg
      421 gcctgctggg caggccgttc aaactctggt tgcttggtgc acaggtccag gaactcctgc
      481 cggaagtact tgatgccgct ggtgatcggg ctggcggcgc cgtccccgag cgcgcagaac
      541 gatcggccca ggatgttgtc acagacgtcc agcatggtct ccacatccga cggggtgccc
      601 tcgcgccgga ccatccgctg caggatttgg gtcagccagt aggtcccctc ccggcacggg
      661 gtgcacttac cgcaggattc gtgcttgtag aactcggtcc acttcatcac cgcccacggc
      721 acgctcaccg tgttgttgaa gaccatgacc gcggtggtgc ccagcatgga gccggcctcc
      781 gcagcgccct cgaagtccag cgggacgtcc atatgctcgt cggtgagcag cggggtcgac
      841 gatccgcccg gcgtccagaa cttcagcggc ccgcccttca tcccgccagc catgtccagc
      901 agctcccgca acgtgatccc caacggggcc tcgtactggc ccggccgctg cacatgcccg
      961 gagatcgagt agatcttggg gccgggcgat ttctcggtgc ccatggtgcg gaaccagtcc
     1021 gacccaccgt tgacgatgta gggcacgctg cagatggtct cgacgttgtt caccacggtg
     1081 ggtgcggcgt acagcccgga gacggccggg aacggcggct tgagccgggg ttccgcccga
     1141 cggccctcta acgagttcag cagcgcggtc tcctccccgg cctcgtaggc cccagcgccc
     1201 gcgtgcacca cgatgtccag gtcaaacccg gagtcgagga tattcttgcc gagatagccg
     1261 gcggcatagg cctccctcac cgcgttgtgc acccggcgca ggcagtgcac cacctcgccg
     1321 cggacgtaga taaagcacag gtgggcccga accgcgtaac tggtgataat gcagccttcg
     1381 atcagcgaat gcgggtcggc catcatcagg ggcacgtcct tgcaggtgcc cggttcgccc
     1441 tcgtctgcgt tgatcaccag gtaggtgggc ttggccgccg gcccggtggg ctgctcgcct
     1501 ggtttggctt gcgggatgaa accccacttc atgccggtgg cgaaaccggc gccgccacgt
     1561 ccgcgcaggc ccgaatcctt gatcaactgg atcaactgat cgggatgcgc ggccagcgcg
     1621 gtgcgcagcg cggtgtagcc ctccagctgg gtgtaggtct cgagcgtcca agacctcggc
     1681 gagagccacc gccgggtcaa caccggggtg agcatccgct cggtcaccgc tgctcctgtc
     1741 gagttcgtca cttcttgtcc gcaacctcag gtagtggcgg ggccttgtcc ggcatcgccg
     1801 gtgcctgcca gcccctgtcc tcggcgagcc ggttgccggc cagcgtctcc ttcgccgccg
     1861 acggcccgtc caccgcgtcc cgcagctcct ggggctcgcc gaagaagccg gctagctgca
     1921 gctcgacggt ccgccaatcg gtcaacggcg caccacgggt cggcgctggc cgatcccccc
     1981 ggcgcagcgc gtccaccagc tccacagccg actccggagt ctggttgtcg tagaactcgt
     2041 agttcacctg caggaccggg gcaaggtcgc aggcggccag gcattcggcg tgctcaaggg
     2101 tgatcgagcc tttcgagcct ggccggcctg cggtctcttc gtggccgata c
//
LOCUS       DATADN010000142         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1070846, whole genome shotgun sequence.
ACCESSION   DATADN010000142 DATADN010000000
VERSION     DATADN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070846"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VHH53_01860"
     CDS             complement(<1..513)
                     /locus_tag="VHH53_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX2288957.1"
                     /translation="MAILIDEHTRVVVQGITGRIGRFHAEEMIDYGTNVVAGVTPGKG
                     GETVLDRPIFNTMKEAVAETGATASIVFVPPPFAADSLMEAADAGIRFCACITDGIPT
                     QDMMKVKRYMWRYKAQQRMTLTGPNCAGTISPGKSLVGIMPGHIYLPGRVGVIGRSGT
                     LGYEAASQMKA"
     gene            complement(528..1706)
                     /locus_tag="VHH53_01865"
     CDS             complement(528..1706)
                     /locus_tag="VHH53_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HEX2288958.1"
                     /translation="MDIHEYQAKEILARFGVPVPRGGLAYSPEQASYRCTEIGGSAWV
                     VKAQVHAGGRGEAGGVKLCSSDREVQDVANSMFGKRLVTKQSGPRGKLIYRVYVEEAT
                     EIARELYFGIVLDRRIERVCIIASAEGGVEIEELLASKPETLIRTTIEPAVGFLEFQA
                     REIAFAVGLQAAQLADASRLFRRAYRAFRDLDATMLEINPLAVTSTGDLVALDAKMSF
                     DDNALFRHLDISELRDRSQEDSRESHAADRGLAYVGLEGNIGCMINGAGLAMATMDMI
                     KLAGGEPANFLDIGGGASPERVLKAFRAVLQDEKVEAMLINIFAGINRCDWVAQGIVA
                     AFKEIDVKIPVVVRLAGTHVEEGQAILRDSGLPIITADTLADAARKAVAVLATAGTSA
                     "
     gene            complement(1716..>2429)
                     /locus_tag="VHH53_01870"
     CDS             complement(1716..>2429)
                     /locus_tag="VHH53_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX2288959.1"
                     /translation="GLDTHYMYRDVVDVMEQAGDRVHTILVPKVGVPADLYCVETLVN
                     QIEQAKGYQIKVGIEALIETALGMANVEAIASSPGRLEALHFGVADYAASNRARTVSI
                     GGLNPDYPGDQWHFALSRMIVACRAYGLRPIDGPFGDYSDPDGFRAGARRAAALGCEG
                     KWAIHPNQVALANEVFSPPEAEVDRAQRIIEALREAEAQGKGAASVDGKMIDAASERM
                     AKTVIAMDEAIRAAAASRT"
BASE COUNT          389 a          858 c          753 g          429 t
ORIGIN      
        1 tgccttcatc tgggacgcgg cctcgtagcc cagggtgccg gagcgcccga tcaccccgac
       61 ccgcccaggc aggtagatgt gcccaggcat gatgcccacc agtgacttcc ccgggctgat
      121 agtgcctgcg cagttggggc cggtaagcgt catccgctgt tgcgccttgt aacgccacat
      181 gtagcgcttg accttcatca tgtcctgcgt cgggatgccg tcggtgatgc aggcgcagaa
      241 ccggatcccg gcgtcggcgg cctccatcag ggagtccgcg gcgaacggcg gcggcacgaa
      301 cacgatggag gcggtggccc cggtctcggc cacggcctcc ttcatggtgt tgaagatcgg
      361 gcggtccagc acggtctcgc cgcctttgcc cggagtgacc ccagcgacca cgttggtgcc
      421 gtagtcgatc atctcctcgg cgtggaagcg tccgatgcga ccggtgatgc cctgcaccac
      481 cacgcgggtg tgctcgtcaa tcaagatggc catgacgcct ccagcactca ggcgctcgta
      541 ccggcggtgg ctagcacagc gacggccttg cgggccgcat cggcgagggt gtccgcagtg
      601 atgatgggca ggccggagtc ccgcaggatc gcctggccct cctcgacgtg ggtgccggcc
      661 agccgcacca cgaccggaat cttgacgtcg atctctttga acgcggcgac gatgccctgg
      721 gcgacccagt cgcagcggtt gatgcccgcg aaaatgttga tcagcatcgc ctcgaccttc
      781 tcgtcctgca gtaccgcgcg gaacgctttg agcacgcgct cgggagatgc tccaccgccg
      841 atgtccagga agttcgccgg ctcgccacca gcgagcttga tcatgtccat ggtcgccatc
      901 gccagaccag cgccattgat catgcagccg atgttgccct cgaggccgac ataggccagc
      961 ccacggtcgg cggcgtgact ttccctgctg tcttcttgac tgcggtcgcg caactcggag
     1021 atgtccagat gtcggaacaa cgcgttatcg tcgaaagaca tcttggcgtc cagcgcgacc
     1081 aggtcgcccg tgctcgtcac ggccagcggg ttgatctcga gcatggtggc gtccagatcc
     1141 cggaaagcac ggtaggcgcg gcgaaacagc cggctcgcgt cggcgagctg ggctgcttgc
     1201 aggcccaccg caaaagcgat ctcccgggcc tgaaactcca ggaaacccac cgcgggttcg
     1261 atcgtcgtgc ggatcaaagt ttcgggcttg gatgcgagca gctcctcgat ctccaccccg
     1321 ccttcggccg aggcgatgat gcagacccgt tcgatgcggc gatcgagcac gatgccgaaa
     1381 tacagctcgc gggcgatctc ggtggcctcc tcgacgtata cccggtagat cagctttccg
     1441 cgcggcccgg actgcttggt gaccagccgc ttgccgaaca tgctgttcgc cacgtcctgc
     1501 acctcacggt cggagctgca gagcttcacc ccgccggcct cgccgcggcc gccagcgtgc
     1561 acctgggcct tgaccaccca ggccgacccg ccgatctcgg tgcaccggta ggacgcctgc
     1621 tcggggctgt aggccaatcc ccctcgcggt acgggtactc cgaaacgggc caggatctcc
     1681 ttggcctggt attcgtgaat gtccacaccc tccccttagg tccggctcgc cgcggcggcc
     1741 cggatcgcct cgtccatcgc gatcaccgtc ttggccatcc gctccgaggc ggcgtcgatc
     1801 attttgccgt ccaccgacgc ggcgcccttg ccctgcgcct cggcctcccg gagcgcctcg
     1861 atgatccgct gggcccgatc cacctcggcc tccggcgggc tgaacacctc attggccagc
     1921 gcgacctggt tggggtgaat cgcccacttg ccctcacacc ccagcgcggc agcccgccga
     1981 gccccggccc ggaaaccatc ggggtcggag tagtcaccaa atggaccgtc aatcggacgc
     2041 aggccataag cccggcatgc cacgatcatc cgcgacaacg cgaaatgcca ctgatcaccg
     2101 gggtagtccg ggttcagacc gccgatgctg accgtgcgag cccggttgct cgccgcgtaa
     2161 tcagccaccc cgaagtgcag cgcctccaac cggccggggc tgctcgcaat ggcctccacg
     2221 tttgccatcc ccagggcggt ctcgatcagc gcctcgatac ccaccttgat ctggtatccc
     2281 ttggcttgct cgatctggtt gaccagtgtc tccacgcagt acaggtcagc gggcaccccc
     2341 accttgggca ccaggatggt gtgtacccgg tcgccggcct gctccatcac gtcgacgacg
     2401 tcgcggtaca tgtagtgggt gtcgagccc
//
LOCUS       DATADN010000143         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_619163, whole genome shotgun sequence.
ACCESSION   DATADN010000143 DATADN010000000
VERSION     DATADN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619163"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /gene="serB"
                     /locus_tag="VHH53_01875"
     CDS             <1..809
                     /gene="serB"
                     /locus_tag="VHH53_01875"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX2288960.1"
                     /translation="VPGNSTREAADTELRATLVRIAARGEVDLAVERVGIARRAKRLV
                     VFDVDSTLVQGEVIEMLAERAGCGTEVSAITEAAMRGELDFTQSLRRRVALLAGLPAS
                     VLSEVTNAIELTAGARTTVRTLKRLGFRCGAVSGGFSQIVRPIAEDLGLDFCAANELE
                     IRDGQLTGQLLGSVLDRSGKADALCRAAEHFGVPLSQCVAVGDGANDIDMLATAGLGV
                     AFNAKPALREIADTAINQPFLDIVLFVLGVTRAEVEAADAADGMLRRVPL"
     gene            855..1625
                     /locus_tag="VHH53_01880"
     CDS             855..1625
                     /locus_tag="VHH53_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="HEX2288961.1"
                     /translation="MPGPLAPLAARYARWLGLPADATADVTDEDPAAVWAMSLILRVE
                     RDAVPLRSALLEAAASAAVALCLDERSQSGGPWNCPMQAWVAGRIRKVTRRARGAHWI
                     AVSDLPGITVKHRGAQVRALVPWPVAATPKTVTRLQVGGTDIPPDDPGPPPAGVPVLW
                     LAPQPVMSTGKAAAQVGHASMLLAALLAADGRAATLDRWAADDYRCAVRTASVQQWAD
                     LVVGKRPDRAWRERGILAVRDAGFTEVPPGTVTVLVSS"
     gene            complement(1603..>2056)
                     /locus_tag="VHH53_01885"
     CDS             complement(1603..>2056)
                     /locus_tag="VHH53_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEX2288962.1"
                     /translation="LPILCQGDDATGLLVAQFAKDEPTCLFGTLSLWQGVDVPGRSLS
                     LVVIDRIPFPRPDDPLSSARQRATAARGGNGFLAVAATHAALLLAQGAGRLLRSTEDR
                     GVVAILDPRLATARYGGFLRASLPPFWPTTDGAVARAALQRLTSSPAR"
BASE COUNT          303 a          667 c          759 g          327 t
ORIGIN      
        1 cggtcccggg aaacagcacc cgggaggctg ctgataccga gttgcgcgcc acgctggtac
       61 ggattgccgc tcgtggggag gtcgaccttg ccgtcgagcg ggttggtatc gctcgtcggg
      121 ccaaacgact ggtggtcttc gacgtcgact ccaccctggt gcagggcgag gtgatcgaga
      181 tgctcgccga acgcgccggt tgcgggactg aggtaagcgc gatcaccgag gctgcgatgc
      241 gcggggagct ggacttcacc cagtcgctga ggcggcgggt tgcgctgctg gcgggcctgc
      301 cagccagtgt gctcagcgag gtgaccaacg cgattgaact cactgccggg gcgcgcacga
      361 cggtgcggac cctcaagcgg ttgggatttc gctgcggagc cgtttcaggt ggtttcagcc
      421 agatcgtccg gccgatcgct gaagacctgg ggctggactt ctgcgctgcc aacgagctcg
      481 agattcgcga cggccagctc actggccagc tcctcgggtc ggtgttggat cggtccggta
      541 aggccgacgc gctgtgccgg gcggcggagc atttcggggt cccgctgtct cagtgtgtcg
      601 ccgtcgggga cggcgccaac gacatcgaca tgcttgccac ggccggtttg ggtgtcgcgt
      661 tcaacgccaa acccgcgttg cgagaaatcg ctgacaccgc catcaaccaa ccgtttctgg
      721 acatcgtgct gttcgtgttg ggggtgaccc gcgcggaggt cgaagcagcc gacgctgccg
      781 acgggatgct gcgaagggtg ccgctgtgac tacggtccct gggagtacgt caggatgact
      841 gtgccggggt gactgtgccg gggccgctgg caccgttggc tgcccgttat gcacgttggc
      901 tgggtctccc ggctgacgcg accgccgacg tgaccgatga ggaccctgcg gcggtgtggg
      961 cgatgtcgct gattctgcgg gtggagcggg atgcggtgcc gctgcgtagc gcgctgctcg
     1021 aggcggctgc ttcggcggcg gttgcgctgt gtctcgacga gcgttcgcaa tccggaggcc
     1081 cgtggaattg tccgatgcag gcttgggtgg caggacggat ccgcaaggtc acccgccgtg
     1141 ctcgcggagc gcactggatc gctgtatcag acctgcccgg catcactgtc aaacatcgcg
     1201 gcgcccaggt gcgggcactg gtgccctggc cagtggccgc cactccgaaa acggtgacgc
     1261 ggctgcaggt cggtggcacc gacatcccgc ccgacgatcc cggcccgccg ccagccggag
     1321 taccggtact ttggctcgcc ccgcagccgg tgatgagcac cggcaaggcc gcggcccagg
     1381 tcggccacgc cagcatgctg ctcgctgcgt tgctggccgc tgacggccgg gccgcaacgc
     1441 tggaccgctg ggcggccgat gactaccgct gtgcggtgcg caccgcctcg gtgcaacagt
     1501 gggctgacct ggtggtaggc aagcgcccgg accgggcctg gcgcgagcgc gggatactgg
     1561 ccgtccgcga tgccgggttc accgaagtcc ctcccggcac cgtcaccgtg ctggtgagct
     1621 cgtgagccgc tgtagcgcgg cccgcgctac agcgccatcc gtggtaggcc agaacggcgg
     1681 tagcgaggcg cgcaggaaac cgccgtaccg ggcggtggcc agccgggggt ccaggatcgc
     1741 gaccacgccg cggtcctcgg tcgaacgcaa cagccggcca gcgccttgcg cgagcagtag
     1801 cgcggcgtgc gtcgcggcca ccgcgaggaa cccgttacca ccgcgagcgg ccgttgcacg
     1861 ctgccgtgcc gagctcagcg ggtcatcagg ccggggaaac gggatgcgat cgatgacgac
     1921 caaagacagc gaccggcccg gcacgtcgac gccctgccac agcgacagcg tgccgaacag
     1981 gcaggtcggc tcgtccttgg cgaactgggc caccagcagg ccggtcgcgt cgtcgccctg
     2041 gcacaggatc ggcagg
//
LOCUS       DATADN010000144         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_920530, whole genome shotgun sequence.
ACCESSION   DATADN010000144 DATADN010000000
VERSION     DATADN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920530"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHH53_01890"
     CDS             <1..235
                     /locus_tag="VHH53_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288963.1"
                     /translation="SLRPSLYAEAIDPDNWPGTGLRYLFKICRNLDGMTGCTESGWTN
                     ATWTPPPGTLRWSHTYYWWALTLHRFHGQVLAC"
     gene            complement(211..1140)
                     /locus_tag="VHH53_01895"
     CDS             complement(211..1140)
                     /locus_tag="VHH53_01895"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX2288964.1"
                     /translation="MYRYIHAEKARDASANIAVMCRVLGVSRSGYYGWVGRQLSPPTG
                     RAAADQQLLEQIRHVHARFSYYGSPRIHQHLLIAAGHAYQTSHSAVAAGHRPRLGRRR
                     VARLMRDHGIRARIGKIKSRPRSAPPKRRPEIGDQVQRQFQAVDPNELWFTDITQIRT
                     GQGWLYAAVILDAFNREVISWATAAQDNLDTAVDALAEAIRIRRPDPGTIIHSDRGYQ
                     FTSAAWLDLARSKGLVVSIGERKDCFDNAVMESWLGSFKTEVLYPNGQPATRTQARTQ
                     LFQYIWDYNTHRLHSTLGYVSPKYYAQHASTCP"
     gene            complement(1143..1475)
                     /locus_tag="VHH53_01900"
     CDS             complement(1143..1475)
                     /locus_tag="VHH53_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2288965.1"
                     /translation="MAKVYNQEFKDQIVELHRGGGRTFMDLAKEFDLSATTVANWVRA
                     AKRAEQRRGHRQPSAASKAAGVQKQQPEVESAEAKIARLERELARKNKELEILGKALA
                     FFARRMDR"
BASE COUNT          311 a          579 c          539 g          337 t
ORIGIN      
        1 gtctctgcgg ccgagcttgt acgccgaggc catcgaccca gacaactggc ccggtaccgg
       61 cctgcggtac ctgttcaaaa tctgccgcaa cttggatggg atgaccgggt gtacggagtc
      121 gggctggacc aatgcaacct ggacaccacc ccctggcacg ttgagatgga gccataccta
      181 ctactggtgg gccctgacct tgcaccggtt tcacggacag gtactggcgt gttgagcgta
      241 gtacttcgga ctgacgtagc ctagtgtcga gtgtagacga tgtgtgttgt agtcccagat
      301 gtattggaac agctgggttc tggcctgcgt gcgggtggct ggttgaccgt tggggtagag
      361 gacttcggtc ttgaaggagc caagccagga ctccatcaca gcgttgtcga agcagtcttt
      421 gcgttctccg atggagacca cgagtccctt gctcctggcc aggtccagcc atgccgcgga
      481 ggtaaactgg tagccgcgat cggagtggat gatcgtgccc gggtccggcc ggcggatacg
      541 gatcgcctcg gccagcgcgt ccacagcagt gtcgagattg tcctgagctg ctgtcgccca
      601 actgatgacc tctcggttga acgcgtcgag gatcaccgcg gcgtaaagcc atccctgccc
      661 ggtacggatc tgagtgatat cggtaaacca caactcgttc ggatccaccg cctggaactg
      721 tcgttggacc tggtcgccga tctccggtcg ccgtttcggc ggagccgaac gcggccgaga
      781 cttgatcttc ccgatccggg ctcggatgcc gtggtcccgc atcaacctgg ccacccgccg
      841 ccggcccagc cgtggcctgt ggcctgcagc cacggcgctg tgactggtct gataggcgtg
      901 tccagccgct atcagcaaat gttggtgaat tcgcggtgat ccgtagtagc tgaaccgtgc
      961 atgcacgtgc cgtatctgct ccagcaactg ctggtcagcg gcggctcgac cggtcggcgg
     1021 cgacagctga cggccaaccc agccgtagta gcccgagcgt gaaacgccaa gaacccggca
     1081 catgacggcg atgttcgccg aggcgtcgcg ggccttctcc gcatgaatgt aacgatagat
     1141 gcctaccggt ccatcctgcg tgcgaagaag gccagcgctt ttcccagaat ctccaactcc
     1201 ttgttcttgc gcgccagttc ccgttccagc cgggcgatct ttgcttctgc ggactcaacc
     1261 tccggctgct gcttctggac gccagccgcc ttcgacgcag ccgagggctg ccggtggccc
     1321 cgccgctgct ctgcccgctt cgccgcgcga acccagttcg ccaccgtcgt ggcagacagg
     1381 tcaaactcct tcgccagatc catgaacgtc cgaccgcccc cacgatgcag ctcaacgatc
     1441 tgatctttga actcctggtt gtaaaccttg gccacaagga cacccctccg cggtcatcaa
     1501 gaccactctg ctggtgtccg cgggaccggt gcaaggtcac ccgggcccac gacaacgtcg
     1561 aagccggtcc gtgggtaggt ccgctggccc tgaccacgac ggtgccgcaa cctgaaatca
     1621 cctcgcacct gggcggaacc ccggacggcg ctccggcgct gggtctggac ccgcaggtgg
     1681 gcaactacgg catggcgagc accgacgcga tggtcgccac cgtcggcccc gatctgacga
     1741 taacccgcac ctacaacagc ctggac
//
LOCUS       DATADN010000145         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1217834, whole genome shotgun sequence.
ACCESSION   DATADN010000145 DATADN010000000
VERSION     DATADN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217834"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..524)
                     /locus_tag="VHH53_01905"
     CDS             complement(42..524)
                     /locus_tag="VHH53_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2288966.1"
                     /translation="MLPAEVLFETRPRKARRVAVVTAVLLVAVFTVIAVLLRQTPTGV
                     YFRLSDQVAMVLLGLLLAGAALLFARPRVRAGADGVEVRNLLGSRTLPWELVLRVSFP
                     DGAPWARLELPDDEYIAVMAIQAADGRHAVHAIRALRAVYAEHTAPPGAPSRDLQPGQ
                     "
     gene            complement(604..1095)
                     /gene="ribH"
                     /locus_tag="VHH53_01910"
     CDS             complement(604..1095)
                     /gene="ribH"
                     /locus_tag="VHH53_01910"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX2288967.1"
                     /translation="MSGQGQPALTVPGGCGELRLGIAVTRWYERVTAALLDRACAAAA
                     EAGITAPTVVRVPGAVELPVVCQELARHHDAVVALGVVIRGGTPHFEYVCDAVTAGLT
                     RVALDEGTAVGNGVLTCDTEEQALDRCGLASSSEDKGFQACVAALETALLLRSLRQSR
                     NTP"
     gene            complement(1092..2335)
                     /locus_tag="VHH53_01915"
                     /pseudo
     CDS             complement(1092..2335)
                     /locus_tag="VHH53_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005152327.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
     gene            complement(2370..>2510)
                     /locus_tag="VHH53_01920"
     CDS             complement(2370..>2510)
                     /locus_tag="VHH53_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX2288968.1"
                     /translation="PTTRELTTLGTRHPGELVNLEADVIAKYIERLAWPHLGSGSGPA
                     AT"
BASE COUNT          404 a          900 c          838 g          368 t
ORIGIN      
        1 accagttacc gccggactgc ggcaatcctg caaccgcctc ctcattggcc cggctgaaga
       61 tcccgactag gtgctccggg tggcgcggtg tgctcggcgt acaccgcgcg cagggcgcgg
      121 attgcgtgca ccgcgtgccg accgtcagca gcctggatcg ccatcaccgc gatgtactcg
      181 tcgtcgggca gctccagacg ggcccacggg gcaccatcgg gaaaagacac ccggagcacc
      241 agttcccagg gcagcgtgcg cgacccgagg aggttgcgca cctccacccc gtcggcaccg
      301 gcacgcactc gaggccgggc gaacaacagc gctgcaccgg cgagtaacag tcccagcagc
      361 accatggcca cctgatcgga gagccggaag tagacgccgg tgggggtctg ccgcagtagc
      421 accgcgatca ctgtgaacac cgcgaccagc aacacggcgg taaccaccgc gacgcggcgg
      481 gccttgcgcg ggcgggtctc gaacagcacc tcggcgggca gcatcacgcc gcctctgccg
      541 tacgccagag cacgacacac cgggcacagc aagcggacac gccgggaagg ggggtgtgcg
      601 gggtcatggt gtgttccgag attggcgcag gctgcgcagc agcagggcgg tctccagagc
      661 ggcgacgcag gcctggaagc ccttgtcctc tgacgaggaa gccagcccgc atcggtccag
      721 ggcttgttcc tcggtgtcgc aggtgagaac cccgttgcct accgcagttc cttcgtccag
      781 cgcgaccctg gtgagtccgg cagtgaccgc gtcgcagacg tactcgaaat gcggggtgcc
      841 accgcggatc accactccca gcgcgaccac ggcatcgtgg tggcgggcca gttcctggca
      901 caccactggc agctcgacgg cgcccggcac tcgcaccacg gtcggcgcgg tgatcccggc
      961 ctcggcagcg gcggcgcacg cccggtcaag cagtgcggcg gtaacgcgtt cataccagcg
     1021 agtcacggcg atacccagcc gcagctcgcc gcagccgccg ggcacggtga gcgcgggctg
     1081 gccctggcca ctcatgccag caccccgctc gacccttcca ggccggtgag gtcatggccc
     1141 atgcggtcgc gcttggtacg caggtaacgc agattctccg ggttgggccg gaccggcaac
     1201 gacacccggt caacgatctc cagaccgtag ccttccaacg cggtccgctt agtggggttg
     1261 ttgctgagca ggcgcatcga gcgcaccccg agatccacca ggatctgcgc tccggtgccg
     1321 tagtcgcggg catcggccgg catcccgagc gcgaggttgg cgtccacggt gtccgcgccg
     1381 gcctcctgca gctggtaggc ctgcagcttg tgcatcaatc cgatgccgcg gccctcatgc
     1441 ccgcggatgt agacgaccac gccgcggccc tcggcggcga ccgcggccag cgcggcatcg
     1501 agctgggtgc cgcagtcgca gcgcagggaa ccgaagatgt cgccggtgag gcactcggag
     1561 tgcatccgca ccaacacgtt ggccccgtcg ccgatctctc cgcggaccag cgcgatgtgc
     1621 tctagcccgt ccagcaggct gtcgaagccc accgcccgga agtcgccgtg gcgggtgggg
     1681 atccgcgcct cggcgacccg ctcgatctgc ttctcgaacc gccggcggta agcgatcatg
     1741 tccgcgatgg tgatcagcgc taggcgatgc tcggcggcga agacctccag ctcgtcgcga
     1801 cgcgccatgt cgccgtcgtc tttggccgag acgatctcgc acagcacgcc ggccgggggc
     1861 aggccggcca accgggccag gtccaccgcg gcctccgtgt gccccgggcg gcgcagcacc
     1921 ccaccctcct tggcccgcag gggcacgacg tgccccgggc ggacgaagtc gaccgacttg
     1981 gcgtccggat cggccagtag ccgaatggtg cgggcccggt ccgtcgctga gatcccggtc
     2041 gtcacgccct cgcgggcatc gaccgtgacg gtgtggcagt gccccgcttg tcctggttga
     2101 tgtgatacat cggcggcagt tccagccggt cacagtcgtc tccggtcagc gccacgcaga
     2161 tgtatcccga ggtgtaccgg accatgaatg ccagtagttc aggagtggcc cgggccgcgg
     2221 ccaagatcag gtcgccctcg ttctcccgct cctggtcgtc caccacgatc accgggcgcc
     2281 cagccgccac ctcggcgatc gcccgatcga tgccggcaaa ggcgttctcg gtcatcgtgt
     2341 cctcctcccc cacatcatcc ccccaaacat catgtcgcag ccggaccgct accggagccg
     2401 agatgtggcc aggccaacct ctcgatatat ttggcgatca cgtcagcctc gaggttcacc
     2461 aactcaccgg gatgccgggt gcccagggtg gtgagctcgc gggtggtagg
//
LOCUS       DATADN010000146         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_166733, whole genome shotgun sequence.
ACCESSION   DATADN010000146 DATADN010000000
VERSION     DATADN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166733"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1656)
                     /locus_tag="VHH53_01925"
     CDS             complement(193..1656)
                     /locus_tag="VHH53_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX2288969.1"
                     /translation="MQIVHSSRKGSRDIEHIGSAHDDVELELLKTVSRQRLAAGQSEL
                     DLGLDAPPETGGPLPITASRMGHLWDALGSVYDALGFADAADGDEVFRQLVLARIIEP
                     TSKLDSLRVLTEAGIAPVSYRTLKRRLPAYAADAWRERLAALCAHHVSLGPATLLLYD
                     VTTLYFETDAGDGFRESGFSKERRLEPQITVGLLTDAAGFPLMVNAFEGNKAETTTML
                     PVIKAFMAAHRLKDVVIVADAGMISENNMKAIEAAGLSFILGMKIPQVPHVVAEWRKA
                     HPDTEITDGQVFVQPWPAGPTDKRTDQVRYYQYKHDRARRTLKGIDEQVRKAEQAVAG
                     KTAVKRNRFVKLTGAAKAVNRELEAKARALAGIKGYVTNIADPTSEQVIGAYQRLFQI
                     EKSFRMAKSDLAARPIYHRTKDSIEAHLTIVFAALAVSHWIEDTTGWSVKKFVKTVRR
                     YRTIQIQAGKHVITAADPLPDDVHQTLEAIKTRAATH"
BASE COUNT          288 a          600 c          623 g          354 t
ORIGIN      
        1 ggtgtgagtg ccccgtggtc tggtgctttc cggagtacag ttcaggtgcc tcggcccaag
       61 accagcaggg acacagcgat ccgtccacca cgacgacgcg tccgttcatc tcctccgtga
      121 gatcggggac gaactcctct agcgcttctg tgatgatctc ttcgaggtcg ctgatacccg
      181 agttgggcca agttagtgcg tagcggctcg ggtcttgatc gcttcgaggg tctggtggac
      241 gtcgtcgggt agcgggtcgg ccgcggtgat cacgtgcttg ccggcctgga tctggatggt
      301 gcggtagcgg cggacggtct tgacgaactt cttgactgac cagccggtgg tgtcctcgat
      361 ccagtgggag acggccaggg cggcgaatac gatcgtcagg tgtgcctcga tcgagtcttt
      421 ggtgcggtgg taaatcgggc gggcagccag gtccgatttg gccatccgga agctcttctc
      481 gatctggaac agccgttggt aggcgccgat gacctgctcg gacgtgggat cggcgatatt
      541 cgtgacgtag cccttgatcc cggccagcgc gcgggccttg gcctccaatt cgcgattgac
      601 ggccttggcg gcgccggtga gtttgacgaa ccggttgcgc ttgaccgcgg tcttgccggc
      661 gacggcctgc tcggccttac ggacctgctc gtcaatgccc ttgagcgtgc gccgggctcg
      721 atcgtgcttg tactggtagt accgcacttg gtccgtgcgt ttgtccgtcg gcccggctgg
      781 ccacggctgg acgaacacct gcccgtcggt gatctcggtg tccgggtggg ccttgcgcca
      841 ctccgcgacg acgtggggga cctgggggat cttcatcccg aggatgaacg acaggcctgc
      901 ggcctcgatc gccttcatgt tgttctcgga gatcatcccg gcatcggcga cgatgaccac
      961 atctttcagg cggtgcgcgg ccatgaacgc cttgatcacc ggcagcatcg tggtggtctc
     1021 ggccttgttg ccctcgaacg cgttgaccat cagcggaaac ccggccgcgt cggtcaacag
     1081 gccgacggtg atctggggtt ccaggcgacg ctccttggag aacccggact cgcggaaccc
     1141 gtcaccggcg tcggtctcga agtacagggt tgtcacgtcg tagagcagca aggtcgccgg
     1201 tcccaggctg acatggtggg cgcacagcgc cgccagccgc tctcgccacg catcggcggc
     1261 gtacgcgggc aggcgccgct tgagggtccg gtaggacacc ggcgcgatgc cggcctcggt
     1321 gagcacccgc agcgagtcca gcttgctggt cggctcgatg atccgcgcca gcaccagctg
     1381 ccggaagacc tcgtctccgt cggccgcgtc ggcgaacccc agcgcgtcat agacgctgcc
     1441 cagggcgtcc cacaggtgcc ccatccgcga ggctgtgatc ggcaacggac cgcccgtctc
     1501 cggcggcgcg tccaacccga ggtcaagctc gctctgcccg gccgccagcc gctgacggga
     1561 cacggtcttg agcaactcga gttcgacatc gtcgtgggcc gacccaatgt gctcgatgtc
     1621 ccgtgagcct ttgcgtgagg agtgcacgat ctgcaccgca cgccccccga agcggtcttc
     1681 acggtccgga cgtaggccac caggccacct taagacccag gattagtgca caaattccgc
     1741 gcgccccgcg acgaacccgc aggtcagcga agcgggctgc tcctcagacc ggaatcgtgg
     1801 cccaagtcag gtttctcgaa atggtcagac gctccaatga acccggctgg gggcgtcgct
     1861 atacc
//
LOCUS       DATADN010000147         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_468736, whole genome shotgun sequence.
ACCESSION   DATADN010000147 DATADN010000000
VERSION     DATADN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468736"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1291
                     /locus_tag="VHH53_01930"
     CDS             134..1291
                     /locus_tag="VHH53_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2288970.1"
                     /translation="MLTTLAVENYRSLRRLVLPLRRLNVVIGANGSGKSSLYRALRLL
                     AESARNGAVAALAREGGLPSTLWAGPEHLGRSVRDGRYPVQGTVRTGPVSLRLGFAGD
                     EFGYAMDLGLPAPLESQFNLDPEIKCEAVWSGPVLRPATLLTERRGAAVRIRDADGSW
                     QTTPHVLQPFDSMLSELADPHRTPELLAVRERIRSWRFYDHVRTDAHAPARITQVGTR
                     TPVLSHDGADLAAALQTIREIGARSALDKAVDQAFPGSRVEIVNSDGRFDLALHQHGL
                     LRPLRSAELSDGTLRFLLWVAALLTPRPPALLVLNEPETSLHPDLLPPLADLISAGAE
                     RMQVVVVTHSRALLAALDQSVHTIELVKEFGQTRMAGQNSLDEPPWHWPKR"
     gene            complement(1415..1488)
                     /locus_tag="VHH53_01935"
     tRNA            complement(1415..1488)
                     /locus_tag="VHH53_01935"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1452..1454),aa:Pro,seq:tgg)
     gene            1564..1634
                     /locus_tag="VHH53_01940"
     tRNA            1564..1634
                     /locus_tag="VHH53_01940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1596..1598,aa:Gly,seq:tcc)
BASE COUNT          248 a          598 c          595 g          257 t
ORIGIN      
        1 attcggtcag ggagaacgct gagctgatga acaccgatct ccccgggctg cccgcgcggt
       61 gcgactcgtg agccgcgtac ggcgccgcac gccggctgac ggcggcaggc cggcgagcac
      121 ctaggctcgg tgggtgctga ccacgctggc ggtggagaac taccggtcgt tgcggcggct
      181 ggtgctcccg ttgcgccggc tcaacgtggt gatcggcgcg aacggcagcg gcaagtccag
      241 cttgtaccgg gccttgcggt tgctggccga gtcggcgcgc aacggagcgg tcgcggcgct
      301 ggcccgggag ggtggactgc cgtccacgct gtgggccggg ccggaacacc tgggccggtc
      361 cgtgcgggat ggcaggtatc cggtgcaggg caccgtgcgg accggtccgg tgagcctgcg
      421 gctgggcttc gcgggcgacg agttcggcta cgccatggat ctggggctgc cggctccgct
      481 ggagtcccag ttcaacctcg atccagagat caagtgtgag gcggtgtgga gcgggccggt
      541 gctgcggccg gccacgctgc tgaccgagcg gcgcggcgcc gccgtacgca tccgcgacgc
      601 ggatggctcc tggcagacca cgccgcacgt actgcagccg ttcgacagca tgctcagcga
      661 gctcgccgat ccgcaccgga cgccggaact gctggccgtg cgcgagcgga tccggtcgtg
      721 gcggttctac gaccacgtcc gcaccgacgc ccacgcaccg gcgcgaatca cccaggtggg
      781 cacccggacg cccgtactgt cacatgacgg ggcggacctg gccgcggccc tgcagacgat
      841 ccgggagatc ggtgcccgct ccgccctgga caaggccgtg gaccaggcgt ttcccggcag
      901 ccgggtggag atcgtcaact ccgacggccg gttcgatctc gcgctgcacc agcacggcct
      961 gctgcgcccg ctgcgcagcg ccgagctgtc cgacgggacc ctacgtttcc tgctgtgggt
     1021 ggccgcgctg ctcactccgc ggccaccggc gctgttggtg ctcaacgaac cggaaaccag
     1081 cctgcacccc gacctgctgc cgccgctggc cgatctgatc agcgccgggg ccgagcgaat
     1141 gcaggtcgtc gtggtcaccc actcccgcgc cctgctcgcc gcgctggacc aaagcgtgca
     1201 caccatcgag ttggtcaagg agttcggcca gacccggatg gccgggcaga actccctcga
     1261 cgaaccgccg tggcactggc cgaaacgctg accaccaggt cgcgtagcgg caaggcctcg
     1321 ttgggcgagg tctgcgggct ccgcaccgga gacccggaga cgatcagttc cgcgcgttcc
     1381 gaacgttccg cggacaggct tcactgaaac cgagtcgggc tgacaggatt tgaacctgcg
     1441 accctccgct cccaaagcgg atgcgctacc aagctgcgcc acagcccgcc acaccacctg
     1501 aggtgctgcg gcaacagcct agcgcgacac gctaggctgt ttgcgcgcca ctacccggcg
     1561 cgcgcgggtg tagctcaatg gcagagcccc agccttccaa gctggtcatg cgggttcgat
     1621 tcccgtcacc cgctcgttcg tgggcctccc ctgctcgcgg ggagcgctcg cctggctcac
     1681 tcgccatttc tttggggg
//
LOCUS       DATADN010000148         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1071614, whole genome shotgun sequence.
ACCESSION   DATADN010000148 DATADN010000000
VERSION     DATADN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071614"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VHH53_01945"
     CDS             complement(<1..686)
                     /locus_tag="VHH53_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEX2288971.1"
                     /translation="MSPHWHPVGNSASRCTSFPPAGACSPVRWRLRRQRSRSRTRNAG
                     VNTRYRGGVRWRFLLRPGWLALTAVVVSFSVAAFTLLAPWQFGRAEQRAERNAAIERS
                     FSIPPQPLREVLAAGAAPAQGTEWRQVVITGRYLPNAETVVRLRTVQGEPAYEVLVPL
                     ELADGSTVLVDRGYLRPVEGRVPAYPPVPGSEVTVTGRLRADEPDPQGGAVVAQDGHR
                     QVYAVNTRTV"
     gene            complement(694..831)
                     /locus_tag="VHH53_01950"
                     /pseudo
     CDS             complement(694..831)
                     /locus_tag="VHH53_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
     gene            complement(858..1394)
                     /locus_tag="VHH53_01955"
                     /pseudo
     CDS             complement(858..1394)
                     /locus_tag="VHH53_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
     gene            complement(1391..1870)
                     /locus_tag="VHH53_01960"
     CDS             complement(1391..1870)
                     /locus_tag="VHH53_01960"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX2288972.1"
                     /translation="MSTAQKRLSVCFVCTGNICRSPMAEIVFTEALRQEGLADRVRVS
                     SAGTGDWHVGGPADPRSAETLRRAGYDCKHVAAQVSAQHLSADLVVALDSGHLAALRR
                     LVAQPDRVVLLRSFDPAADGDFDVPDPYFGGKHGFDEVLGMVHAAVPGLLAWVRSRL"
BASE COUNT          321 a          774 c          671 g          272 t
ORIGIN      
        1 ctcacggtgc gggtattcac cgcgtagacc tgccggtgac cgtcctgggc cacgactgca
       61 ccaccctgcg ggtccggctc gtcggctcgc aaccggccgg tcacggtcac ctcgctaccg
      121 ggcaccgggg gataggcagg cacccgcccc tcgaccgggc gtagataacc gcgatctacg
      181 agcacggtcg atccgtccgc caactcgagc gggaccagca cctcgtacgc cggctcgccc
      241 tgcacggtgc gcagccgcac gaccgtctcg gcgttgggca ggtagcgccc ggtgatgacg
      301 acctgccgcc actcggtgcc ctgcgccggc gccgcgccgg cggcaagtac ttcgcgtagc
      361 ggctgcggcg gaatgctgaa ggagcgctcg atcgcggcgt tgcgctcggc acgctgctcc
      421 gcacgcccga actgccaggg cgcaagcagg gtgaatgcgg cgacggaaaa gctcaccacc
      481 accgccgtca gcgccaacca gccaggacgc agcagaaacc gccaccgcac cccaccacgg
      541 taacgggtgt tcaccccagc gttgcgcgtg cggctgcggc tgcgctgacg ccgtagccgc
      601 caccgaacag gactgcatgc accagcagga ggaaagctgg tgcagcgcga cgcgctgttg
      661 ccaaccggat gccagtgggg cgacatcctg gtaaccggcg agcaactgct ccaggtgcga
      721 gaggccgaac agggcgagca tcgccaggtc ggtttcccgg tgcccgccgt gcgccgcggg
      781 gtcgagcagc catacctgcc cgtcgcgccg gcgtgcacgt tgcccgccca cagatcacca
      841 tgcagccggg ccggtggctc ggccggcccg gccagctcgt cgatccgctc acagacctgg
      901 tgcaccgcac gggccccggt ggcgtccacg gcgccacgat ccacggcact gcgcaagtag
      961 cgcaacaccc gatgctcggc ataccactgc ggccattgca ccccggtgac gttggccatc
     1021 ggggcggcac cgatccacgc ttcatgcggt ccgtccggcg gtgcggcgcc gaaggcagga
     1081 gctcccgcgg tgtgcagcgt ggcgagctga cgcccaaggc gctcagccgc cttggcgtcc
     1141 agccggccgg ccggcacgtg ttcggtgacc agccactgcc cgtcgtgtcc gtgcacctgc
     1201 ggcaccggtg gaccgccggg tacctgtagc cagcgcagcc cggccgcctc ggccaggacc
     1261 tgcccgggtt caccccgctt ggccaccacg gtgcgcccgt cacccaactc gatcaaccag
     1321 atgctgccgc gctgggtcac agcgtgcact ggctgcccgg taatcgcagc gacggcctcg
     1381 ccgatcgcag tcatagccgg ctgcgtaccc acgccagcaa cccgggcacc gccgcgtgca
     1441 ccatgccgag tacctcgtcg aagccgtgct tgccaccgaa gtacgggtcg ggcacgtcga
     1501 aatccccgtc cgccgccggg tcgaatgatc gcagcagcac cacgcggtcc ggttgcgcca
     1561 ccagccggcg cagtgccgcg agatgcccgg aatccaacgc caccaccaga tcggcggaca
     1621 agtgctgcgc gctgacctgc gcggcgacgt gcttgcagtc gtagccggct cggcgcagcg
     1681 tctcggcgct gcggggatcg gccggaccgc cgacgtgcca gtcaccggtt cccgcgctgc
     1741 tcacccgcac ccggtcggcc agaccttcct ggcgcagcgc ctccgtgaag acgatctcgg
     1801 ccatcgggga gcggcagatg ttgccggtgc agacgaagca gaccgacagc ctcttttgcg
     1861 ctgtgctcac aaccccagtc tgaacccaca gcacgcaagc cctacggggg cagctgcggc
     1921 acaaccgccc cactgccgcc agtcgcgccg tccgcgtgac cgggtccttc cgcgaaacgt
     1981 cgtcgtacag cgcgtgacgt gcccgcaggc gacctggatg gctctgcatg gtcctggc
//
LOCUS       DATADN010000149         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_770150, whole genome shotgun sequence.
ACCESSION   DATADN010000149 DATADN010000000
VERSION     DATADN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770150"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VHH53_01965"
     CDS             complement(<1..812)
                     /locus_tag="VHH53_01965"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX2288973.1"
                     /translation="MSWLQALVLGLVQGLTEFLPISSSGHLRIVSEIFFGDDAGAAFT
                     AVTQLGTEAAVLIYFARDIGHLVATWFRGFVSAQVRATPDYRIAWLVIIGTIPIGVLG
                     FIFEDQIQTSGRNLWIIGSTLIVFGLVLAAAERYGQQRRRLQQLTVTDGVVMGLAQAM
                     ALVPGVSRSGGTISAGLFLGLTRPTAVRYSFLLAIPAVIAAGIFQIPDVFTVGGPGLQ
                     PTVAQMVVATLLAAAVGYAAIAWLLRYVERHSVYLFVWYRLALGTTVLALLAT"
     gene            868..1830
                     /locus_tag="VHH53_01970"
     CDS             868..1830
                     /locus_tag="VHH53_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2288974.1"
                     /translation="MQQRLLGRSGLRVSRLALGTMTWGCDTDAEEAATQLVAFIDAGG
                     TLVDTADVYNEGESERILGELLADVIPRDEVVLATKAVARRDDGPFGGGASRAALLAA
                     LEGSLRRLRTDHVDLWQLHAWDPTVPLEETLSAVTDAVRCGKVRYVGVSNYTGWQLAT
                     AKALCDVPLVSTQVEYSLLERGVERELAPAAAYHGVGLLPWAPLARGVLTGKYRNGTP
                     SDSRAASPHFARYVEHHRSDRSTRIVQAVVTAADGLGTSPLEVALAWVRDRPGVVAPV
                     VGARNTAQLAGSLAAEEVELAPAIRAALDDVSEVQLGYPERPLG"
BASE COUNT          294 a          669 c          705 g          265 t
ORIGIN      
        1 gtggccagca gcgccagcac ggtggtcccc agggccagcc ggtaccagac gaacaggtac
       61 acgctgtggc gctcgacgta gcgcagcaac cacgcgatcg cggcgtaacc taccgccgcc
      121 gcaagcaggg tggcgaccac catctgggcc accgtgggtt gcaggccggg gccgccgacg
      181 gtgaagacgt cggggatctg gaagatcccg gcggcgatga cggcggggat cgcgagcagg
      241 aatgagtagc gcaccgcggt gggccgggtc agcccgagga acaggccggc gctgatcgtt
      301 ccgccggagc gcgacacccc gggcaccagc gccatggcct gggccaagcc catcaccacc
      361 ccgtcggtga cggtcagctg ctggagtcgc cgtcgctgct ggccgtagcg ctccgcggcg
      421 gccagcacca gaccgaacac gatcagcgtg ctgccgatga tccacaggtt tcgccccgag
      481 gtctggatct ggtcttcgaa aataaagccc agcacgccga tcgggatcgt tccgatgatc
      541 accagccagg cgatccggta atccggggtg gcgcggacct gcgccgaaac aaacccgcgg
      601 aaccaggtcg ccaccaggtg gccgatgtcg cgggcgaagt agatcagcac tgccgcctcg
      661 gtacccagct gggtgaccgc ggtgaacgct gcgccggcgt cgtcgccgaa gaagatctcc
      721 gagacgatcc gcaggtgccc tgacgaggag atcgggagga actcggtgag gccctgaacg
      781 agtcccagga ccagggcttg cagccagctc acgcggctgc accctacggg tcccggcgcc
      841 ggcaccagca ggcgctagcg tgcccccgtg caacagcgtc tccttggccg cagcgggctc
      901 cgggtgtccc ggctggcgct gggcaccatg acgtggggct gtgacaccga cgccgaggag
      961 gccgccaccc agctggtcgc gttcatcgat gccggcggca cgctggtgga caccgcggac
     1021 gtctacaacg agggcgagtc tgagcggatc cttggtgagt tgctggccga tgtcatcccc
     1081 cgagatgagg tggtgctggc gaccaaggcg gtggcgcgcc gcgatgacgg gccattcggt
     1141 ggtggcgcgt cccgcgcggc gctgctcgcg gcgttggaag gttcgcttcg ccgccttcgc
     1201 accgaccacg tcgacctgtg gcagctgcac gcctgggatc ccacggtgcc gctggaggag
     1261 acgctgtccg cggtgaccga cgcggtgcgc tgcggcaagg tgcgctacgt cggggtgtcg
     1321 aactacaccg gctggcagct ggccaccgcg aaggccctct gcgacgtgcc gctggtgtcg
     1381 acccaggtgg agtactcgct gctggagcgg ggggtggaac gcgagctggc gcccgcggcg
     1441 gcctatcacg gggtgggcct gctgccctgg gcgccgctgg cccgcggggt gctcaccggc
     1501 aagtaccgca acggcacccc ctctgattcc cgggcggcct cgccacactt cgcgcgctac
     1561 gtcgagcacc accgcagcga ccggtcgacc cggatcgtgc aggccgtggt caccgcggcc
     1621 gacgggctag gcacctcccc gctggaggtg gcactcgcct gggtccggga ccggcccggg
     1681 gtggtcgcgc cggtcgtcgg tgcccgcaac accgcgcagc tggccggctc gctggcagcc
     1741 gaagaggtgg agttggcccc ggccatccga gctgcgctgg atgacgtcag tgaggtgcag
     1801 ctgggatatc cggagcgccc gctcggttga gccgccgcat cgccgcggcc atgtcacgcg
     1861 ccgctcgcac cgtgccccgg accgatccgg acaccttgga tcgcccgccg gcgcgcgggc
     1921 ggtactcgac gtc
//
LOCUS       DATADN010000150         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_468889, whole genome shotgun sequence.
ACCESSION   DATADN010000150 DATADN010000000
VERSION     DATADN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468889"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VHH53_01975"
     CDS             complement(<1..554)
                     /locus_tag="VHH53_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2288975.1"
                     /translation="MRATLIVNPHSGRGAAVRVAGTVAATLRAAVPGLTATVTADAAA
                     TSRLAEQAARRGDDVLAVLGGDGTVHLAAQAVAWTETALAVLPSGTGNDLATALGIPA
                     DPVHAARHVADALRSGRRRRIDLGRVEGGAYCATVLCTGFDAAVNARANAMRWPAGPR
                     RYDLAVLAELAALTPRPVRVHAGDR"
     gene            complement(573..746)
                     /locus_tag="VHH53_01980"
     CDS             complement(573..746)
                     /locus_tag="VHH53_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288976.1"
                     /translation="MAGNERCSRLPTINSAIRATNVQRRHHYGHGGAGINLSGDSRTE
                     ITDLLRTAPSPHP"
     gene            909..1064
                     /locus_tag="VHH53_01985"
     CDS             909..1064
                     /locus_tag="VHH53_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288977.1"
                     /translation="MAESEDKEQHGEDSQTAADAAEDQRINDPLTRTDVKARRVPFGP
                     QPSSEES"
BASE COUNT          251 a          509 c          542 g          247 t
ORIGIN      
        1 cggtccccgg cgtgcacccg gaccggccgc ggggtcagcg ccgcgagctc ggcgagcacc
       61 gccaggtcgt agcgtcgtgg tcccgccggc cagcgcatcg cattggctcg ggcgttcacc
      121 gcggcatcga agccggtgca cagcaccgtg gcgcagtaag caccgccctc cacacggccg
      181 aggtcgatgc gccggcgccg cccgctgcgc agcgcgtcgg cgacgtgccg ggcggcgtgc
      241 accgggtcgg cagggatgcc cagcgcagtg gccaggtcgt tgccggtccc gctgggtagc
      301 accgccagcg cggtctcagt ccaggcgacg gcctgagcgg ccaggtgcac ggtgccgtcc
      361 ccgcccagca ctgccagcac gtcgtcaccg cgtcgcgccg cttgctcggc cagcctgctg
      421 gtcgccgccg cgtcggcagt cacggtggcc gtcagcccgg gtaccgcggc gcgcagcgtg
      481 gccgccacgg tgccggccac gcggaccgcc gcaccacggc cggaatgggg gttgacgatc
      541 agcgtggcgc gcacggctgc ggatactacg gctcacgggt gtggggatgg tgcggtgcgg
      601 agtagatcgg tgatctcggt gcgcgaatcc ccagaaaggt tgatcccggc tccgccgtgg
      661 ccgtagtggt ggcgccgctg cacattggtg gcccggatcg ccgagttgat cgtggggagg
      721 cgcgaacacc gctcattccc cgccaccgtc gagctgacct cgcgccgtcc gcgtgagcac
      781 ggttgagcga tcggttgcga cttcgctgag tacaaggaga agttgcccga aaggctctag
      841 ccgctcgcta ggctacgacg cgcggaagaa ccactcaacg acaacaacac aacgcctagg
      901 agctgaacat ggccgaatcc gaagataagg agcagcacgg cgaagattcg cagaccgctg
      961 ccgatgctgc ggaggaccag cgcattaacg atccgcttac tcgaaccgat gtgaaggcac
     1021 gtcgtgttcc cttcggaccc cagccgtcgt cggaggagtc atagatccac tatgtgcctg
     1081 aaagagatcc gcttgcagta gaccgtcggg ccagcgatgg ccgccgcgag gccgcgccac
     1141 aacaggttcc tccggcgggg acggcggtta actggtgcgc ttgtagtagt tggaatctcg
     1201 ttctgtcagc gctgctcgtg atctagctgt gcatgcccgc cgatcagcgg gggagcacca
     1261 gcccaggtct gacgagggtg actgtgatcg agacttgacc atctcgtagg aagcacttag
     1321 gtgcgttact gccgtaccga gctgggacgt acgtgcttta tcgcccggtc acagaccaaa
     1381 tgcatggatc actctcatcg ggataactca ggtggcgtcg tcgtagccgg gcgcagcgtt
     1441 ctccttcgac accggctcga cctgatggtc cagtggggag ggttcgtcgg gatcgaggtc
     1501 gccctgctcg gcgcgccgcc gggccagggt gcgatcgtgc gcccgagcg
//
LOCUS       DATADN010000151         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_15975, whole genome shotgun sequence.
ACCESSION   DATADN010000151 DATADN010000000
VERSION     DATADN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15975"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VHH53_01990"
     CDS             <1..356
                     /locus_tag="VHH53_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288978.1"
                     /translation="PARGVQAELFQRPLDQRLAYARTPRVIDEVAVYLLPLGALTDDV
                     IAQARARHSIDGGDRIAVPATDPSGAGRRRGLEPGIASGVNGLGSRRRGPVAGCAGPG
                     DRAVPAGRSSWSSRS"
     gene            complement(373..978)
                     /locus_tag="VHH53_01995"
     CDS             complement(373..978)
                     /locus_tag="VHH53_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288979.1"
                     /translation="MEPVWIIIFGIVAIAVVGLVGMMIIWQDARGKLQQRVGPEYDRH
                     VRARGSKRHAKRRLSDTADRRDRVDIRPLRPAARERYLRRWEAVQTGFVDRPGPALDE
                     ADRLVMDVMHDRGYPVSDFAERAELAAADYPEVVKHYHAAHAARQSNHGHPSPSDTEH
                     LRQAFVHYRMLFEALVRDGKQGDRTAQQAPAQTPPPPPTRG"
     gene            1333..1488
                     /locus_tag="VHH53_02000"
     CDS             1333..1488
                     /locus_tag="VHH53_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HEX2288980.1"
                     /translation="MIILGLILTVVGVLAGIPVLYIIGIILLVIGVILALFGLTGRAI
                     GGRRYYF"
BASE COUNT          247 a          488 c          523 g          292 t
ORIGIN      
        1 tgcccgctcg aggggttcaa gcggagcttt ttcaacgtcc gctcgaccag cggctcgcct
       61 atgcgcgcac gccgcgggtc atcgacgaag tcgccgtgta cttgttgcca ctgggtgcac
      121 tgaccgacga cgtcattgcg caggcccgag cgcggcacag catcgacggg ggcgaccgga
      181 tcgccgtgcc ggccaccgac ccctcgggcg ccgggcggcg gaggggactg gagcccggga
      241 tcgcgtccgg cgtcaacggg ctcggcagtc gccggcgggg acccgtggcc gggtgcgccg
      301 ggccggggga tcgggctgtt cctgcggggc gttcatcctg gagctcccgt tcgtgatgct
      361 cgacgtgcca gcctatccgc gggtgggcgg gggcggtggg gtctgcgcgg gcgcctgctg
      421 agcagtgcgg tcaccctgct tgccgtctcg cacaagtgct tcgaacagca tgcggtagtg
      481 cacgaacgcc tgccgcaagt gctcggtatc ggacgggctt gggtgcccgt gattgctctg
      541 ccgggcggca tgggcggcgt ggtagtgctt cacgacttcg gggtagtcgg cggcggcgag
      601 ctcggcacgt tcggcgaagt cgcttaccgg gtagccacga tcgtgcatca catccatgac
      661 aagccggtct gcctcgtcaa gggcaggacc aggccggtca acgaagccgg tttgcacggc
      721 ttcccagcgt cggaggtaac gctcgcgggc cgcgggcctc agcgggcgga tatcaactcg
      781 gtcgcgccga tctgcggtgt cgcttaggcg ccgcttcgca tggcgcttgc taccgcgcgc
      841 acgcacgtgg cggtcgtact caggtccgac tcgttgttgt agcttgccac gggcatcctg
      901 ccaaatgatc atcattccga ccaatccgac gaccgcaatc gcgacgatgc cgaagataat
      961 gatccagacc ggttccataa caagcctcct cccccgttca agtgatcagc actcgcccat
     1021 tcggcctgta cccggcaacc gttcggggca cataatagct tcaccaagat gatgtcgcat
     1081 gttcaacgac ggagtcccgt agccagggtt tcaacgccgc gctgctgcta gcaccagcgg
     1141 atgagcatca cgcgggtcgc cgtgcgcgag caggtcaaca gggcctaccg gtagcgcccg
     1201 gtgggacctg ggcagcaggc gccgaccggc ccgacggctc cgggaagcta gatgcttgcg
     1261 tttcggaagg cgaaatcggc aggtgtgaca tgcactccac aaaggacgcc acacggaatc
     1321 cccggagaaa tcatgatcat ccttggtctc atcctcaccg tggttggggt tctggccggg
     1381 atcccggttc tgtacataat cggtatcatc ctgttggtga ttggggtgat cctggcgctg
     1441 tttgggctca ccggacgtgc cattggtggc cgacggtact atttctaact ctttctaccg
     1501 cttcctgttc gatccgacgg ctgcggctgg gaaaacgcca tcgtcggtgg
//
LOCUS       DATADN010000152         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1372200, whole genome shotgun sequence.
ACCESSION   DATADN010000152 DATADN010000000
VERSION     DATADN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372200"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VHH53_02005"
     CDS             complement(<1..188)
                     /locus_tag="VHH53_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288981.1"
                     /translation="MSDRPHRRRRPARVTAAVIVLPVLLALLTGARTVPAAVTWQHLD
                     PVELITTIDLPALQASNRV"
     gene            384..1367
                     /locus_tag="VHH53_02010"
     CDS             384..1367
                     /locus_tag="VHH53_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX2288982.1"
                     /translation="MSTRFSRAVVTGGAGFLGSHLCEQLLRRGTGVVCLDNFLTGAPA
                     NIAHLIGEPRFRVIKCDVTDFVHVPGEVDLVLHLASAASPADYLRLPIETLKVGSLGT
                     LHALGLAAEKGARFVLASTSEVYGDPQQHPQRESYWGNVNPVGPRSVYDEAKRYAEAL
                     TTAYRMSRGTDTGIVRIFNTYGPRMRADDGRAIPTFICQALAGEPLPVTGDGSQTRSV
                     CYVEDTIRGILALAESDAAGPVNIGAPYELPVTELAETIIALSGCPGSIRYIDSPVDD
                     PRVRRPDITLAWDLLGWRPEVTLANGLTRTIDWFRAELPGTVDPFREARSG"
     gene            1440..>1686
                     /locus_tag="VHH53_02015"
     CDS             1440..>1686
                     /locus_tag="VHH53_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2288983.1"
                     /translation="MRILGINAIFHDPAAALVIDGEIVAAAEEERFNRRKHGKRPVPF
                     SAWELPEQSARWCLDVAGIGPQDLDAVAYSYDPALVQP"
BASE COUNT          264 a          562 c          575 g          285 t
ORIGIN      
        1 acccggttcg acgcctgcag tgctggcagg tcgatggtgg tgatcagctc gactgggtcg
       61 aggtgctgcc aggtcacggc ggccgggacg gtccgcgcgc cggtcagcag agcgagtaat
      121 acgggcagga cgataactgc ggcggtgacg cgcgcagggc ggcgccgccg gtggggacga
      181 tccgacaatg cttcactcct tcaccccctt tctggtgctg gcgccttgtt gacgccgtgc
      241 tgccggcggg ccgtccccga gacggccatc cattcggcga gcaggctatg gccaccgccg
      301 ccgttggtga atagcccgtt tggtgcggtt atgttgtggg tactactcct gtggccacac
      361 agggcagcgg tcaagggggt ccggtgagca ccaggttcag cagggccgtg gtcaccggag
      421 gagccggatt cctgggctcc catctgtgcg aacagttgtt gcggcgaggc accggcgtcg
      481 tgtgcctgga caacttcctc accggcgcac cggccaatat cgcccacctg atcggcgaac
      541 cgcgattccg cgtgatcaag tgtgatgtga ccgacttcgt gcacgtgcca ggcgaggtgg
      601 acctggtgct gcacctggcc tcggctgcat cccccgccga ctatctccgg ctgcccatcg
      661 agacgctcaa ggtcggcagc ttgggcacct tgcatgcgtt gggcctggcc gcggagaagg
      721 gcgcccggtt cgtgctggcc tccaccagcg aggtctacgg cgacccgcag cagcatccgc
      781 agcgcgagag ttactgggga aacgtcaacc cggtcgggcc gcgcagcgtg tatgacgagg
      841 ccaaacgcta cgccgaggcg ctgaccaccg cctaccgcat gtcccgcggc acggacaccg
      901 gaatcgtccg gatcttcaac acctacggcc cccggatgcg cgcggacgac ggcagggcca
      961 tcccgacctt catctgccag gccctggcgg gtgagccgct gccggtcacc ggcgacggca
     1021 gccagacccg ttcggtgtgc tacgtcgagg acacgatccg cggcatcctc gccctggccg
     1081 agtccgacgc cgccggaccg gtgaacatcg gcgcaccgta cgagctgccg gtgaccgagc
     1141 tggccgagac gatcatcgcg ttgagcggct gccccgggtc gatccgctac atcgacagtc
     1201 ccgtcgacga tccgcgagtt cggcgcccgg acatcacgct ggcctgggac ctccttggct
     1261 ggcgaccgga ggtcaccctc gctaacgggc tgacccgtac catcgactgg ttccgggctg
     1321 agctgcccgg cacggttgat ccgtttcgag aggcgcgatc cggatagcaa tgcgatgctt
     1381 tccgggtagc agtgctggtg agaggcctta ttccgcgatc aaccaggaga gggaggccgg
     1441 tgcgcattct cggcatcaac gcgattttcc acgatccggc ggccgcattg gtcatcgatg
     1501 gggagatcgt ggcggctgcc gaggaggagc ggttcaaccg tcgcaagcac ggtaagcgcc
     1561 cggtgccgtt ctcggcctgg gaactgcccg agcagtccgc ccggtggtgt ctggacgttg
     1621 ccggcatcgg cccgcaggac ctcgacgccg ttgcctactc ctacgacccc gcactggttc
     1681 aaccgg
//
LOCUS       DATADN010000153         3972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_318617, whole genome shotgun sequence.
ACCESSION   DATADN010000153 DATADN010000000
VERSION     DATADN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318617"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VHH53_02020"
     CDS             <1..1375
                     /locus_tag="VHH53_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX2288984.1"
                     /translation="DAPEIQYMWERRRALGGFVPERRTKFKPLVLPGDKSYDVLRRGS
                     GKQEVATTMAFVRQLRELIKDPEIGPRFVPIIPDEARTFGMDSMFPTQKIYNPHGQLY
                     TSVDAELMLAYRESEQGQILHEGINEAGSTASFTAAGTSYATHNEPLIPVYIFYSMFG
                     FQRTGDGLWAAGDQMARGFLLGATAGRTTLVGEGLQHNDGHSLLLAASNPAVVAYDPA
                     WGFEIAHIVRDGLRRMYGDDGARDPNVCYYLTIYNEPYRQPAEPEDLDVEALLRGLYR
                     YAPAPAPGRDKPAVQLAASGVALPWALRAQAMLAQEWGVQADVWSATSWTELRREAVE
                     VDRHNLLHPEAEPRVPHVTWALQKAAGPLVAVSDWMRAVPDLIRPWVPTDLVTLGTDG
                     FGFSDTRPAARRYFLVDAESITVAALMALARRGEVEQSVVAEASQRYRIDNPQAAGPQ
                     TSDPGVA"
     gene            complement(1399..2190)
                     /locus_tag="VHH53_02025"
     CDS             complement(1399..2190)
                     /locus_tag="VHH53_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX2288985.1"
                     /translation="MVSPAPKLEPKVDVRRADQRKHTNLDWLDSWHCFSFRRHYDPAN
                     IHFGLLLVSNDDVVKPGMGFETHPHRDMEIVTWVLEGSLVHEDSKSNTGVIYPGLAQR
                     MSAGTGIQHSEKNDSWRLDDEPHDQDVHFVQMWMPPDNRSIEPSYEQLDINRELGPVD
                     AGSGDLVVVASGMDVHRDRRAIFIQQQHAALHAARLQSGASVELPAAAFVHLFVARGA
                     VDLEGTGLLDTGDAARMTAAGGQRVTAGANGAEVLVWEMAARLTG"
     gene            2273..3457
                     /locus_tag="VHH53_02030"
     CDS             2273..3457
                     /locus_tag="VHH53_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2288986.1"
                     /translation="MTVQTGIHPLPASTLRRLGRASGGLATASVAEMERRYPWFRRLP
                     ADHRAGVLLVTQASVANFVEWLKDPQHSIKLTIDAFRTAPRDLTRWVSLRQTVELVRV
                     AALVFEQRLPELAADDAERAVLTEASLRYSREVAFAAASTYADAAEARGAWDARLEAL
                     VVDGIMRGDGEEWLLSRAAALGLDPAAPATVLVGNPPPDEPPELLSDIRSLAIRTGRG
                     ILVGVQGSRLVLVVSGRPDLPEDSILMMEAFGEGPVVIGPTVPSLAEAHRSAADALSA
                     LRAVAAWPAAPRPVPSADLLPERALAGDPEAERLLVDQVMRPLVAASGSLIETLDAFF
                     DAGGVLESCARQLFVHPNTVRYRLRRITDITGRVPGNPRDALVLRVGLAVGRLARSRG
                     LW"
     gene            3633..>3972
                     /locus_tag="VHH53_02035"
     CDS             3633..>3972
                     /locus_tag="VHH53_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX2288987.1"
                     /translation="MLTPWLALEGAPARVAAWSKATGLDLARLGTTAEAEEIKDTAVT
                     QPLVVALSLLAAEELAHRIDLPANAPVAGHSVGEITAAAIAGVLTADEAVAFAAIRGR
                     EMATACALQPT"
BASE COUNT          630 a         1393 c         1309 g          640 t
ORIGIN      
        1 ggacgcaccg gagatccagt acatgtggga gcggcgccgc gcactgggcg ggttcgtgcc
       61 cgagcgacgc accaagttca agcccctcgt gctgcccggc gacaagtcct acgacgtgct
      121 gcggcgcggg tcaggtaagc aggaggtcgc caccacgatg gcgttcgtcc ggcagctgcg
      181 cgagctgatc aaggatcctg agatcgggcc ccgcttcgtg ccgatcatcc cggacgaggc
      241 acgcaccttc ggcatggatt cgatgttccc gacgcagaag atctacaacc cgcacgggca
      301 gctctacacc tcggtggacg ccgagttgat gctggcctac cgggaaagcg agcagggcca
      361 gatcctgcac gaggggatca acgaggcggg ctccaccgcg tcgttcaccg cggcgggcac
      421 ctcctacgcc acgcacaatg agccactaat cccggtctac atcttctact cgatgttcgg
      481 tttccagcgc accggcgacg gcttgtgggc agccggcgac cagatggccc gcgggttcct
      541 gcttggcgcg accgccggac gtaccacgct ggtcggcgag gggctgcagc acaacgacgg
      601 gcactccctg ctgctcgctg cgtccaatcc cgccgtggtg gcctacgacc cggcgtgggg
      661 cttcgaaatc gcccacatcg tgcgcgacgg gctgcgccgg atgtatggcg atgatggagc
      721 gcgcgatccg aacgtctgct actacctcac catctacaac gagccctacc gccagccagc
      781 agagcccgag gacctcgacg tcgaggccct gctgcgtggc ctgtaccgct acgcaccggc
      841 accagcaccg ggccgcgaca agccggccgt gcagctcgcc gcctccggag ttgcgctgcc
      901 gtgggcgctc cgggcccagg cgatgctggc ccaggagtgg ggcgtgcagg ccgacgtgtg
      961 gtcggcgaca tcctggacgg agctgcgccg ggaggctgtc gaggtcgatc ggcacaatct
     1021 gctgcatccc gaggccgagc cccgggtgcc ccacgtgacc tgggcgctgc aaaaggccgc
     1081 gggcccgctg gtcgcggtgt cagactggat gcgtgcggta cccgacctca tccgtccctg
     1141 ggtgcccacc gacctggtaa ccctcggcac ggacggtttc ggcttctccg acactcgccc
     1201 agcggcccgc cggtacttcc tcgtcgacgc cgagtcgatc actgtggccg cgctgatggc
     1261 cctggctcgc cgcggcgaag tggaacagtc cgtcgtcgcc gaggcaagcc agcgctaccg
     1321 catcgacaac ccgcaagccg cgggccccca aacctccgac cctggagtgg cctagttgcg
     1381 ggcagggcgc ggggttggct aaccggtgag gcgggcagcc atctcccaga cgagcacctc
     1441 ggcgccgttg gctccagcgg tgactcgctg gccaccggcg gcggtcatcc gggccgcatc
     1501 gccggtgtcg agcagaccgg tgccctccag atccactgcg ccgcgggcga cgaacaggtg
     1561 cacgaacgcc gcagccggca attcgacgct tgcgcccgat tggagccgtg cggcgtgcag
     1621 cgccgcatgc tgctgctgaa tgaagatggc ccgacggtcc cggtgaacgt ccatcccgga
     1681 cgccaccacc acaagatcac cggatccggc atcaactgga cccaactcgc ggttgatgtc
     1741 cagttgctca tagctgggtt cgatcgaacg gttgtccgga ggcatccaca tctgcacgaa
     1801 atgcacatcc tgatcatgcg gctcatcgtc taagcgccag gagtcattct tctcggaatg
     1861 ctggattcca gtaccggcgc tcatacgctg cgccaacccg ggatagatca cgccggtgtt
     1921 gctcttggaa tcctcgtgta ccagggaacc ctccagcacc cacgtgacaa tctccatatc
     1981 tcgatgcggg tgggtctcga atcccatccc gggcttcacg acatcatcgt tgctcaccag
     2041 tagcaggccg aagtggatat tggccggatc gtagtggcgc cggaaggaaa aacagtgcca
     2101 ggagtccagc cagtcgaggt tggtgtgctt gcgctgatcc gcccgacgca cgtcgacctt
     2161 gggttcgagc ttcggtgccg ggctgaccat cttctctcct caggcttcgg gctgttcagg
     2221 cgtttcaaac actacccagg tggccgctgc agcgccgtgc gatgctggcc ccatgaccgt
     2281 ccagaccggc atccacccgc tgccggcgag caccctgcgc cggctgggac gggcatccgg
     2341 tgggctcgcc acggccagcg tggccgagat ggagcgccgc tacccgtggt ttcgccggct
     2401 gcctgccgat caccgcgccg gcgttttgct ggtcactcag gccagcgtgg cgaatttcgt
     2461 cgagtggctc aaggatccgc agcactcgat caagctcacc atcgacgcgt tccgcacagc
     2521 cccgcgtgat ctcacccgct gggtcagcct gcggcagacc gtggagctgg tccgggtcgc
     2581 cgccctggtc ttcgagcaac gactgcctga gcttgccgcc gacgacgccg aacgcgccgt
     2641 actgaccgag gcctcgctgc gctacagccg cgaggtcgcg ttcgccgccg ccagcaccta
     2701 cgccgatgcc gccgaggccc ggggggcttg ggacgcccgg cttgaagcac tggtggtgga
     2761 cggcatcatg cgcggagacg gcgaggagtg gctactgtcc cgcgccgcgg cgctgggttt
     2821 ggacccagcg gccccggcca cggtactggt gggcaacccg cctccggacg agccgccgga
     2881 actgctgtcc gacatccgca gtctcgccat ccgcaccggc cggggcattc tcgtcggcgt
     2941 gcagggatcc cgcctggtac tggtcgtcag cggccgccct gacctacccg aggactccat
     3001 tctgatgatg gaggccttcg gcgaaggtcc agtggtgatc gggccgacgg tgccatcact
     3061 ggccgaagct catcgcagcg ccgccgacgc gttgtccgca ctgcgcgccg tagcggcctg
     3121 gcccgcggcg ccccgaccgg taccgtccgc cgacctgctc cccgagcgcg ccctggccgg
     3181 ggacccggag gccgaacggc tgctggtcga ccaggtgatg cgtccgctgg tggcggccag
     3241 cggcagcctg atcgagactc tcgacgcctt cttcgacgcc ggcggggtcc tggagagctg
     3301 cgcccggcag ctgttcgtcc accccaatac ggtccgctac cggttgcgcc ggatcaccga
     3361 catcaccggt cgggtgccgg gcaacccgcg agatgcactg gtgctacggg tcggcctcgc
     3421 ggtcggccgg ctggcccgtt cccgcggcct gtggtaacga acaaccacat accgcctaat
     3481 taggcctggt cccggcaact cgttgtagac actccacaag aacggcggcc tggacttcgt
     3541 gcccgtcagc atcacgcagt ttccgtacct ggtgttcagt agagctggtg atcgcgctgc
     3601 ttgcccccgg acaaggtgct cagtcccctg gcatgctcac accgtggctc gcgctggagg
     3661 gtgcaccggc ccgagttgcc gcgtggtcga aggcgaccgg gcttgatcta gcacggttgg
     3721 gcactacggc ggaagccgag gagatcaagg acaccgcagt gactcagccg ctggtggtgg
     3781 cgctgtcgct gctcgcggcc gaggagttgg cccaccgaat cgacctgccg gccaacgccc
     3841 cggtcgcggg ccactcggtc ggggagatca ccgcagcagc gatagccgga gtactcaccg
     3901 cggacgaggc cgtggctttc gccgccatcc gcggccgcga gatggccaca gcctgcgcgc
     3961 tacaaccaac cg
//
LOCUS       DATADN010000154         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1372392, whole genome shotgun sequence.
ACCESSION   DATADN010000154 DATADN010000000
VERSION     DATADN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372392"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="VHH53_02040"
     CDS             complement(<1..1432)
                     /locus_tag="VHH53_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288988.1"
                     /translation="MVPIPDEITITRQAREPVWDPTLGIAVQAAWQGTPQHRLVSIGD
                     SLTQGFASGAVFQTDLSYSAIIAYELGWDGLRYPRYGGPGGLPLNIELLLRELERDFG
                     AELSWWELPMALFRGRQFLDQVEDYWERGLGAVLPAPTATNHVLGVYGWDLRDVLVRT
                     ADSSAAEITQPKDDLLSQTVENNNARAALRVLPRATEADRRRTLLDAAASLGNELGDK
                     DAGRDPDHGIETLVVFVGANNALGAVGELRVVWSGPGFDDLARKDAFTVWQPSHFIVE
                     LNRVVEKVQAIRARHVIWCTIPHVTIAPIARGVAGKVRPDSRYFPYYTRPWISDRDFD
                     PGRDPAITEQQARAVDSAIDQYNDAITAAVADARRAGRDWYLLDVAGVLDRLASRRYL
                     DNVEARPPWWRPYPLPPELAALRPVPDSRFLTSDGTGRRSGGLFSLDGIHPTTVGYGI
                     IAQELIDIMVRAGVQFMAPDGRTPRIG"
     gene            complement(1482..2057)
                     /locus_tag="VHH53_02045"
     CDS             complement(1482..2057)
                     /locus_tag="VHH53_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288989.1"
                     /translation="MVICDRQGDELLLSLSPEGVRFLREQWSWLRELVRWQLGGCTDD
                     PLSQLTGLPVPTSPIDGRLAYVVDYWCGTEETGPVRQVWEGWVLHDLEHSLSRVLGTL
                     PRHGGVVQLPGQGYPAAAEWGWMLETLHVVLDVSGRVSTQGAPERMLPPPPSAQDTDS
                     YRRSLRWLEMVLDALAQAGQAGRAAPTGATD"
     gene            complement(2113..>2299)
                     /locus_tag="VHH53_02050"
     CDS             complement(2113..>2299)
                     /locus_tag="VHH53_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEX2288990.1"
                     /translation="DGAVVSAGGRVLTVVGTGIDLAAARAQAYQRVEQVQLPGGHYRS
                     DIGLHATRRESWPVAKG"
BASE COUNT          378 a          797 c          758 g          366 t
ORIGIN      
        1 gcccgatgcg cggagtgcgc ccgtcgggcg ccatgaattg caccccggcg cgaaccatga
       61 tgtcgatcag ctcctgggcg atgatcccgt agcccaccgt ggtcgggtgt atcccgtcca
      121 acgagaacaa ccccccgctg cgccgcccgg tgccgtcgct ggtgagaaac cgtgagtccg
      181 gcaccggacg cagtgccgcc agctcgggtg gtagcgggta gggccgccac cacggtggtc
      241 gggcctccac gttgtccagg taccgccggg aggcgagccg gtcgagcact cccgcgacat
      301 ccagcaagta ccagtcccgg ccagcccggc gggcatcggc gacggcagcg gtgatggcgt
      361 cgttgtactg gtctatggca ctgtccacgg cccgcgcctg ttgctcggtg atcgcgggat
      421 cgcggccggg gtcgaaatca cgatcggaga tccacgggcg ggtgtagtac gggaagtacc
      481 gtgagtccgg ccgcaccttg ccggctactc cgcgggcgat cggcgcgatt gtgacgtgcg
      541 ggatcgtgca ccagatgacg tgccgggcgc ggatcgcctg taccttctcg acgacacggt
      601 tgagctcgac gatgaagtgg ctaggttgcc ataccgtgaa agcgtccttg cgcgccaggt
      661 cgtcgaagcc tggaccgctc cacaccaccc gcagctcgcc gacggcgccc agcgcgttgt
      721 tggcaccgac gaacaccacg agcgtctcga tgccgtgatc gggatcgcgc ccggcatcct
      781 tgtcccccaa ctcattaccc aagctggcgg ccgcgtcgag cagggtgcgc cgccggtcgg
      841 cttcggtggc ccgcggcaga acccgtaacg ccgcccgggc gttattgttc tccacggtct
      901 gggacagcag gtcgtccttg ggctgcgtga tctcggccgc ggagctgtcc gcagtgcgga
      961 ccaagacgtc acgcaggtcc cagccgtaga cgccgagcac gtggttggtg gcggtcgggg
     1021 cgggcagcac agcgccgaga ccacgttccc agtagtcctc cacctggtcc aggaactggc
     1081 ggccgcgaaa cagcgccatg ggcagttccc accagctcag ctcggcaccg aagtcccgct
     1141 cgagctcgcg cagcagtagt tcgatgttca gcggaagccc accggggccg ccgtagcgcg
     1201 ggtaacgcag cccatcccaa cccagctcgt aggcgatgat cgccgagtag gacagatcgg
     1261 tctggaacac agcgccactg gcaaagcctt gcgtcaacga gtccccgata ctgaccagcc
     1321 ggtgctgcgg ggtgccctgc caggcagcct ggaccgcgat gcccaacgtc ggatcccaga
     1381 ccggctctcg agcctgcctg gtgatcgtga tctcatccgg tataggcacc atggcctgcc
     1441 cctctcgagg cgttgacagc cggtatgtct actgctggag gtcagtcggt ggcgcccgtc
     1501 ggggcggccc gcccagcctg ccctgcctgc gccagcgcgt cgagcaccat ttccagccaa
     1561 cgcaggctgc gccggtagct gtcagtgtcc tgagccgatg gcggtggtgg cagcatgcgc
     1621 tccggtgcac cttgggtact gacccggccg gagacgtcca gtacaacgtg cagcgtttcc
     1681 aacatccagc cccattcggc ggcggccgga taaccctgac cgggtagctg gaccacgccg
     1741 ccgtggcggg gcagcgtacc gagcacccgg gacaggctgt gctccaagtc gtgcagtacc
     1801 cagccctccc agacctggcg gaccgggccg gtctcctccg tgccgcacca gtaatccacg
     1861 acgtaggcca accggccgtc gataggactg gtaggtaccg gtagtccggt gagctgggac
     1921 agtgggtcat cggtgcaacc gccgagctgc cagcgcacca gctctcgcaa ccaagaccat
     1981 tgttcgcgga gaaagcggac gccttccggc gacagcgaca gcagcaattc gtcgccctgg
     2041 cggtcgcata tgaccatcgt ctcgctcggt ctccacttct tccgcagtga tcgcgtcggg
     2101 gaggttatcg cgtcaaccct tggcgacagg ccaagactcg cggcgcgtcg catgcaatcc
     2161 gatatcactg cggtaatgac cgcccgggag ctggacctgc tcgacgcgct ggtaggcctg
     2221 cgctcgcgcg gcagccagat cgatgcccgt tccgaccacc gtgagcaccc gtcccccggc
     2281 cgacacgacg gcgccgtca
//
LOCUS       DATADN010000155         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1219448, whole genome shotgun sequence.
ACCESSION   DATADN010000155 DATADN010000000
VERSION     DATADN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219448"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1979
                     /gene="dnaG"
                     /locus_tag="VHH53_02055"
     CDS             99..1979
                     /gene="dnaG"
                     /locus_tag="VHH53_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX2288991.1"
                     /translation="MAGRIRDADIAEVRERNRIDEVVGEYVALRRAGADSLKGLCPFH
                     DEKTSSFNVRPGHGTFHCFGCGEGGSVVDFMMKIEHLGFVEAVERLADRVGIQLTYTG
                     GGSSVQRDRGTRARLVEANKLAQEFYADQLSSPEARPAREFLTERGFGQEVAGTFGCG
                     FAPSGWEQLTKHLLGRGFSFEELAKAGLSREGQRGPMDRFHRRLLWPIRDLGGDVVGF
                     GARRLFNDDRNEAKYLNTAETPLYRKSQVLFGLDLAKREIGRRHQVVVVEGYTDVMAM
                     HLSGVPTAVASCGTAFGAEHISVLRRLLMDDDALSGEVIFVFDGDEAGQKAALKAFDG
                     DQRFAAQTFIAIAPDKMDPCELRQAKGDAAVADLVARRQPLFTFAVRTLLAEHDLDTA
                     EGQIAALRRAVPLVAQIKDVSLRDRYATQLSGWVGWTGDEATVVRRVREAAGDEPRQR
                     GGRKRSTSPAENGTETPPRDDVRLQAQREVLKAVLQEPAMTGPVYDTLPAEAFTHPSY
                     AQLHAAVLAAGGTAAGLSGAEWIDAVAAHYPQRSLLTELAVETLRAKGSAEDDARYIG
                     SLLARLQATVLGRQVAEIKSRLQRISPVEDADSYHQLFGDLVALEQYYRSLRDQAVGA
                     HE"
     gene            1976..2326
                     /locus_tag="VHH53_02060"
     CDS             1976..2326
                     /locus_tag="VHH53_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288992.1"
                     /translation="MSGWGRLLRRFAAVPDLPTGFTGILAPAERVVTMGQLAGGGHLV
                     LTQLGMWVPEGADARRIGWHLVSKAVWDCSELVVTEAVSTETAGEAVLVSDLPPHRFA
                     LAQPGKVPRWCATG"
BASE COUNT          368 a          795 c          905 g          390 t
ORIGIN      
        1 acccggtgag ccggtaggcg accccgagca ggtggctgcg gtgctgctgg acgacgtccg
       61 cctcggcctg cactcgactg accctagact cgctgaccgt ggctggacgg attcgggacg
      121 ccgacatcgc ggaggtccgc gaacgcaacc ggatcgacga ggttgtcggc gagtacgtcg
      181 ccctgcgccg ggctggcgcg gactcgctca aggggctgtg cccgttccac gacgagaaga
      241 cctcgtcgtt caacgtccgc ccaggccacg ggacctttca ctgcttcggc tgcggcgagg
      301 gtggctccgt tgtcgacttc atgatgaaga tcgagcacct cggtttcgtg gaggccgtgg
      361 agcggctggc ggaccgggtc ggaatccagc tgacgtacac cggcggcggc tcctcggtgc
      421 agcgcgaccg cggcacccga gcccgactcg tcgaggccaa caagctggcg caggagttct
      481 acgccgatca gctcagctcg cccgaggcgc ggccggcccg agagttcctc accgagcggg
      541 gattcggcca ggaggtcgcg ggcactttcg gctgtggctt cgccccatcg ggctgggagc
      601 agctcaccaa gcacctgctc ggccgcgggt tctccttcga ggaactggcc aaggcggggc
      661 tgtcccggga gggccagcgg gggccgatgg atcgcttcca ccgcaggctg ctgtggccga
      721 tccgggacct cggcggggac gtggtgggct tcggcgcccg gcggctgttc aacgacgacc
      781 gcaacgaggc gaagtacctc aacaccgccg agacgccgct gtaccgcaag tcgcaggtgc
      841 ttttcgggct cgatctcgcc aagcgggaga tcggccggcg ccaccaggtc gtcgtggtcg
      901 agggctacac cgacgtgatg gcgatgcacc tgtccggggt gcccaccgcg gtggcgtcct
      961 gcggcacggc gttcggcgcc gagcacatct cggtgctgcg ccggctgctc atggacgacg
     1021 acgccttgag tggcgaggtc atcttcgttt tcgacggtga tgaggccggc cagaaggcgg
     1081 cgctcaaggc gttcgacggt gaccagcgct ttgcggcgca gacctttata gcgatcgcac
     1141 cggacaagat ggacccgtgc gagctgcgcc aggccaaggg cgatgccgcg gtggccgacc
     1201 tggtggcccg ccgccagccg ctgttcacct tcgcggtgcg aaccctgctc gccgagcacg
     1261 acttggacac cgcggagggc cagatcgcag cgctacggcg ggctgtgccg ttggttgctc
     1321 agatcaagga tgtgtcactc cgggatcgat acgcgacgca gctgtcgggc tgggttggct
     1381 ggacggggga cgaagccacg gtggtccggc gggtccggga agccgccggc gacgaacctc
     1441 gacaacgcgg cggccgcaag cggagcacgt cgccggccga gaatggcacg gagacccctc
     1501 cgcgagacga tgtgcggctg caggcccagc gcgaagtgct caaggcggtg ctccaagaac
     1561 ctgcgatgac cggcccggtg tacgacacgc tgcccgccga ggcgttcacg caccccagct
     1621 acgcccagtt gcacgcagcg gtgctggccg cgggtggtac cgctgcaggg ttgtccggtg
     1681 cggagtggat cgatgccgtc gccgcgcact acccgcagcg atcactgctg accgagctcg
     1741 ccgtggagac gttgcgagcc aagggcagcg ccgaggacga cgcgcgctac atcggcagcc
     1801 tgctggcccg gctgcaggcc accgtcctcg ggcggcaggt tgcggagatc aagtcgcggc
     1861 tgcagcggat ctcgccggtg gaggacgcgg attcctacca ccagctcttc ggtgacctgg
     1921 tggctttgga gcagtactac cggtcgcttc gcgaccaagc tgtgggagca catgaatgag
     1981 tggctgggga cgactgttgc ggcgttttgc cgcggtgccg gacctgccca ccggattcac
     2041 cggcattctt gcgcccgccg agcgggtcgt caccatgggg cagctggccg gtggcggcca
     2101 cctggtgctg acccagctgg ggatgtgggt accggagggt gctgatgccc gccgtatcgg
     2161 ctggcacctg gtgagcaagg cggtgtggga ctgcagcgag ctggtggtca ctgaggcggt
     2221 cagcacggag actgcgggtg aagccgtgct ggtctctgat ttaccgccgc accgtttcgc
     2281 gctcgcgcag cccggcaagg tcccgaggtg gtgcgccacc gggtgaacag ctcgatccgg
     2341 tgcagcgagc gctgcaaact tcccggtggc gggccgtggt tcgtccagcg ccggatccct
     2401 ggacgggatg acgtcgtgct gcagatgcgc gccgatctgg gtactgcgct cggcgccg
//
LOCUS       DATADN010000156         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_470185, whole genome shotgun sequence.
ACCESSION   DATADN010000156 DATADN010000000
VERSION     DATADN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470185"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..424
                     /locus_tag="VHH53_02065"
     CDS             35..424
                     /locus_tag="VHH53_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2288993.1"
                     /translation="MVEEFTVQQAFGSVIADRPGLRVWREPGRLSFPNLTITVAEASA
                     GDWQRWFENFVLQGYNNDTREKTGTLTLLNLQEQEQARIGLFNVGIFQLIGPTVWLNR
                     SKQTGQLEHATRVVARLYSERMEFTLP"
     gene            complement(670..1482)
                     /locus_tag="VHH53_02070"
     CDS             complement(670..1482)
                     /locus_tag="VHH53_02070"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX2288994.1"
                     /translation="MRLMRRAATLVVAALSVLLVAGPAGAVVGGTTDTANRYANVGVL
                     QLNVDGEWVDFCTGTLVDADVVLTAAHCTDFLIEEGEDGFGPDDLRISFDPEGDAPYY
                     TVDHIVVHPDWLTAPPGRGNSKMLYLAPPAEDIALVFLDTEVAGVTPAPIADADYLAG
                     LDLTSETFTVVGYGTDAYITGSAMANHPVVLFDGIRSYRDVSVITEHDLFPGRFLKIT
                     AGVCFGDSGGPLFHEDTLVAINAWTFSMRCAGPNLEYRVDSTAAQTFLDTYL"
BASE COUNT          308 a          536 c          511 g          251 t
ORIGIN      
        1 cctggagctc tccgggcttg actgcaggca ggtcatggtc gaggagttca ccgtccagca
       61 ggcattcggg agcgtaatcg ccgacaggcc cggcctccgc gtgtggcgtg agccgggccg
      121 gctgtcgttc ccgaacctga ccatcactgt cgctgaagcc tcagccggag actggcagcg
      181 atggttcgag aacttcgtcc ttcagggtta caacaacgac acccgggaga agacgggcac
      241 cctcacgctg ctgaacctgc aggagcagga acaggcgcgg atcggcctgt tcaacgtcgg
      301 catctttcag ttgatcggtc cgaccgtctg gctcaaccgg tcaaagcaga caggacagct
      361 cgaacatgcg acccgggtcg ttgccaggct ctactccgag cggatggagt tcacgctccc
      421 atagctgaac aagcaaagca acctgaacct aggaccgccc cggcaggccg ccacccaagc
      481 cgccgacctt gaatggtcga gcgctcgtca taacaactgc ccatcgcctt gacacaccgt
      541 agaaccctcc aagggcatcg gatcgtcgtg cgaggttccc gatgagccag acgccaggag
      601 gtcctcgagg ttaaacgatg gtgcgccggc cggtggttcc gccgcgccgg ccggcgcccc
      661 gtcagtcagc tagaggtagg tgtcgaggaa cgtctgggcc gcggtcgagt cgacccggta
      721 ctccaggttc gggcccgcgc accgcatgct gaaggtccag gcattgatgg ccacaagagt
      781 gtcctcgtgg aacaacggcc cacccgagtc gccaaagcag acacccgcgg tgatcttcag
      841 gaaccgcccg ggaaacaagt catgctcggt gatcaccgag acgtcacggt agctgcggat
      901 cccgtcgaac agcaccaccg gatggttggc catggcgctg ccggtgatgt aggcatcggt
      961 gccatagccg acgacggtga acgtctcact cgtcaggtca aggccggcca ggtagtcggc
     1021 gtcggcgatc ggcgccgggg tcacgccggc cacctcggtg tccaggaaca ccagcgcgat
     1081 gtcctcagcc ggtggggcca ggtagagcat cttggagttg ccccgcccgg ggggcgcggt
     1141 cagccagtct gggtgcacca cgatgtgatc gaccgtgtag tagggggcgt caccctccgg
     1201 gtcaaaggag atccgcagat cgtcggggcc aaagccgtcc tcgccctcct cgatcaggaa
     1261 gtcggtgcag tgggcagcgg tcaggacgac atcggcgtcg accagcgtcc cggtgcagaa
     1321 gtcgacccat tcgccgtcga cgttcagctg cagcacgccc acgttggcgt accggttggc
     1381 agtgtcggtg gtcccgccga ccaccgcccc agctggtccg gcgaccagca agacactgag
     1441 cgccgccacc accagcgtcg cggccctgcg catcaaccgc atgttccctc cttcgtcgga
     1501 gtcccactgg agggaacgcc acccggtgac aaacgttacg accgcgcaga gcgatgcaaa
     1561 gatccacccc gcccccaatc gtcaggcaat gcaccctcgg ccgtgg
//
LOCUS       DATADN010000157         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_319592, whole genome shotgun sequence.
ACCESSION   DATADN010000157 DATADN010000000
VERSION     DATADN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319592"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VHH53_02075"
     CDS             complement(<1..606)
                     /locus_tag="VHH53_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2288995.1"
                     /translation="MRLGAIEAGGTKVLCLVGSDPDRIVAQTRIPTGRPTETLAQVLT
                     FFRQQVASGGPLAAVGVTSFGPLELRRSHPRYGSIRNSPKPGWSDVNMVEPVRNALGV
                     PVGFDTDVNGAALGEGRWGAARGLDTFVYLTVGTGIGAGAVVEDRIIHGLGHPEMGHV
                     SVPRQPGDDFAGDCPFHAGCLEGMASGTAIAARWGRPAEQLD"
     gene            720..>2050
                     /locus_tag="VHH53_02080"
     CDS             720..>2050
                     /locus_tag="VHH53_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX2288996.1"
                     /translation="MPDGPFGSGSNPLEDLVSRLVGGLEQSLGGIAKPGTSTQRLDRY
                     GRDLTADARASRLDPVIGREDEIEQVLEVLSRRTKNNPVLIGDPGVGKTAIVEGIAQR
                     IAEGAVPEPLRDRRVIALDLAGMVAGTKYRGEFEERLTAVINEVTAAARTVVLFLDEL
                     HTVIGAGAAEGGAMDAGSMLKPALARGDLQVVGATTVDEYRRHIERDPALERRFAPIL
                     VAEPSVDDTVAILRGLSKRYEVHHRVRVTDGAIDAAARLSHRYLTDRFLPDKAIDLID
                     RASARVRMRAEAPSEDTRALEQRVAQLTRAKDIAIDAEDYERAEALTRELDLAIVELD
                     TVHAGPMRPPEVSAADVADIIARSTGIPVAQLTEVERHRLLNLEEQLRQRVVGQDEAV
                     EAVADAVRASRAGLNQPDRPIGSFLFLGPTGVGKTELARALAQALFGSQEQL"
BASE COUNT          327 a          704 c          733 g          286 t
ORIGIN      
        1 gtcgagctgc tcggcggggc ggccccagcg ggcggcgata gccgtgccgc tcgccatccc
       61 ctccaggcag ccggcatgaa acgggcagtc accggcgaag tcgtcgccgg gctggcgtgg
      121 cacgctgacg tggcccatct cggggtgtcc caaaccgtgg atgatccggt cctccaccac
      181 cgctccggca ccgatgccgg tgccgacggt caggtagacg aaagtatcca gaccacgtgc
      241 agccccccag cggccctcac cgagcgcagc cccgttgacg tcggtgtcga agcccaccgg
      301 cacgcccagt gcgttgcgga ccggctccac catgttgacg tcagaccagc ccggtttcgg
      361 gctgttcctg atggatccat accggggatg ggaccggcgc agttccagcg ggccgaacga
      421 ggtgacgccg accgcggcca acggcccacc ggacgccacc tgctgccgga agaaggtcag
      481 cacctgcgcc agcgtctcgg tgggtctgcc ggtggggatg cgggtctggg ccacgatgcg
      541 gtctggatcg gaaccaacca ggcagaggac cttcgtcccg cctgcctcga tcgcccccaa
      601 tcgcaccggt ctcactttac ggcgaggcga tcggtcgggc ccccagactg agcggctgcg
      661 tgtcgaggcg aacgggcggt accgtcgaca gggaattgtg ctcgacgagg aggctcggta
      721 tgcccgatgg gcctttcggt tccgggtcca acccgctcga ggacctggtg agtcggctgg
      781 tcggcggcct tgagcagtcc cttggtggaa tagccaagcc gggcacatcg acgcagcgcc
      841 tggaccgcta cggccgcgac ctcacggccg atgcccgcgc cagccgcctc gaccccgtca
      901 tcgggcgcga ggacgagatc gaacaagtcc tcgaggtgct gtcgcggcgt accaagaaca
      961 atccggtact gatcggtgat cccggcgtcg ggaagactgc gattgtcgag ggcatcgccc
     1021 agcgaatagc cgagggcgcg gtcccggagc cactgcgcga ccggcgagtg atcgcgctgg
     1081 acttggccgg gatggtggcc ggcaccaagt accggggcga attcgaggag cggctcacag
     1141 cggtgatcaa cgaggtgacg gccgctgccc ggaccgtggt gctgtttctc gacgagctgc
     1201 acacggtgat cggtgccggc gcggccgagg gcggcgcgat ggacgccggc agcatgctca
     1261 agccggcgct ggcccgcggt gacctgcagg tcgtcggagc caccacggtt gacgagtacc
     1321 gccggcacat cgagcgggat ccggcgctgg aacggcggtt tgcccccatc ctggttgccg
     1381 agccgagcgt cgacgacacc gtcgccatcc tgcgcggcct gagcaagcgc tacgaggtcc
     1441 accaccgggt gcgcgtcacc gacggtgcca tcgacgccgc agcgcggcta tctcaccgat
     1501 acctgaccga ccggttcctc ccggacaagg cgatcgacct gatcgaccgg gccagcgccc
     1561 gggtgcggat gcgggcggag gctccgagcg aggacacccg ggcgctcgag cagcgtgtcg
     1621 cgcagctcac ccgggccaag gacatcgcga ttgacgccga ggactacgag cgggcggagg
     1681 cgctgacccg ggagctcgac ctggcgatcg tggaactgga caccgtgcac gccggtccga
     1741 tgcgcccgcc ggaggtcagt gccgccgacg tcgcggacat catcgcccgc agcaccggca
     1801 tcccggtcgc gcagctcacc gaggtggagc ggcaccggct gctcaatctc gaagagcagc
     1861 tacggcagcg ggtcgtcggg caggatgagg cggtcgaggc cgtcgccgac gcggtccgcg
     1921 ccagccgtgc cggtctgaac cagccggacc gcccgatcgg gtcgtttctg ttcctgggcc
     1981 ccaccggggt cggcaagacc gagttggctc gggcgctggc gcaggccctg ttcggatcgc
     2041 aggagcagct
//
LOCUS       DATADN010000158         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_470548, whole genome shotgun sequence.
ACCESSION   DATADN010000158 DATADN010000000
VERSION     DATADN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470548"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..789
                     /locus_tag="VHH53_02085"
                     /pseudo
     CDS             1..789
                     /locus_tag="VHH53_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005167604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(777..1049)
                     /locus_tag="VHH53_02090"
     CDS             complement(777..1049)
                     /locus_tag="VHH53_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2288997.1"
                     /translation="MRTAGIERTFAIQELTLPLALAGDDLIGQARTGTGKTLGFGVPL
                     IQRITAGDITLDGTPRALVVVPTRELCLQVTADLTETGRDLGVRCT"
     gene            1507..2151
                     /locus_tag="VHH53_02095"
     CDS             1507..2151
                     /locus_tag="VHH53_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HEX2288998.1"
                     /translation="MDVVRDGVVDLLGVLTYGKLSAFDRLAEDSRAAPTLTARIAMAT
                     MAAAEIGHFRLLERHLHGMGVSVDDAVRPFVARFDRFHALTAPRSWLESLVKVYVGDG
                     LAFDFYGEVGEWTDDTTAALVKEVLADTGHSAFAEREVRAACERNRTIHDRLTLWGRR
                     LLGEAVTNTQHVMAEREALADLIIRGSGDVSGLATLIRRVQILHCRRMAALGLG"
     gene            2211..2441
                     /locus_tag="VHH53_02100"
     CDS             2211..2441
                     /locus_tag="VHH53_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEX2288999.1"
                     /translation="MEIKIGIADSPRELTVSTADSPDQIETLVTEALRTTQGLLTLID
                     EKGRRYMVPASRVAYVEIGPPDSRRVGFSVGG"
     gene            2497..3195
                     /locus_tag="VHH53_02105"
     CDS             2497..3195
                     /locus_tag="VHH53_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2289000.1"
                     /translation="MPRLLVVEDDETIGSVLESSLRAHGHQVAWQRTGRRALRLAAEA
                     EFDLVLLDLGLPDLDGVEVCRQLRASNPTTVLVILTARTDEMDVVVGLEAGADDYLTK
                     PVRLAELLARVRAHLRRVAPATPGAPPMLTVADLVVDTGSRRVTVAGREVPLRAKEFD
                     LLACLAAAPEVALSRETLMAQVWDENWFGPTKTLDVHVAALRRKLAGMVELGSVPRIV
                     TLRGHGYRLEAPEN"
     gene            complement(3204..>3507)
                     /locus_tag="VHH53_02110"
     CDS             complement(3204..>3507)
                     /locus_tag="VHH53_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX2289001.1"
                     /translation="CLIATAKEGGARSTPEGHTAIVFERNTCSVNGSQAVLLENFSRI
                     IVRDNHFSGAGSRAVQLGMGSTDCSVIGNTMAGSMRPFEIDRPSRPGFQAADNTSR"
BASE COUNT          567 a         1111 c         1230 g          599 t
ORIGIN      
        1 ctcgacggta ccccgcgcgc cttggtggtc gtgcccaccc gtgaactgtg cctccaggtc
       61 accgctgatc tcaccgagac cggtcgtgat ttaggcgtac gggtcttgcc ggtctatggc
      121 ggccggcctt acgaaccaca gatcaaggcg ctgaccagag gggtcgacct ggtcgtcggc
      181 actccgggcc ggctgctgga cctcgccgag cagcgccatc tggtgctggg caaggtacgg
      241 gtgctcgtac tcgacgaagc cgacgagatg ctcgatctgg gcttcctgcc cgacatcgag
      301 cggatcctgc ggatggtgcc cgcgcaacgc cagaccatgc tgttctcggc caccatgccc
      361 ggcccgatca ttacgctggc ccggactttc ctcacccgtc cgacgcatat ccgcgccgag
      421 gagcccgacg ccaccgccat ccacgaacgg acccggcaat tcgtctaccg tgcgcacgcg
      481 atggacaagg tggagctgct cgctcgcacc ttgcaggccc gcgaacgtgg tctgacgatg
      541 atctttacga ggaccaagcg cactgctcaa aaggtggccg acgagctcac cgagcgcggt
      601 ttcgcggccg cggcggtgca cggtgacctc ggtcagggcg cccgcgagca ggccctacgg
      661 gcgttccgct cagcgaaagt cgacgtactc gtcgccaccg acgtggccgc ccggggcatc
      721 gacgttcccg aggtcacgca cgtgatcaat taccagtgtc cggaagatga gaagacctac
      781 gtgcaccgga cgcctaaatc acgaccggtc tcggtgagat cagcggtgac ctggaggcac
      841 agttcacggg tgggcacgac caccaaggcg cgcggggtgc cgtcgagcgt gatatcgccc
      901 gcggtgatcc gctggatcag cggaacaccg aagcccagcg tcttgccggt gccggtgcga
      961 gcctgaccga taaggtcatc gcccgccagg gccagcggca gcgtgagttc ctggatggcg
     1021 aaggtgcgct cgatacctgc ggtgcgcaat gtgcggacga tctcgtcgcg gacaccgagc
     1081 tcggcgaaag taggggagtt gtcccggacc ggagctccgg cctgcagcgg ggctggcccg
     1141 tcgaggggaa tctcagagtg gtcggggctt cgatggtggg gatgctgctg aggttggacg
     1201 tcgggtaggt aggtcggact gacggtcagt ggatcgcctc tctcgtacgg cgcgcccgcc
     1261 gctgtctcgc tcgaccagct ccgggtcgct agtaacgcag cgaagcgggg aacgaggaca
     1321 cgaggtgcgc gctcacatgc gtgcgctcgg cggcatcagc taccgagccg ggcgcgttgg
     1381 ccgccggtgg cgttacgccc tgtacacata tcatacccct cccacggcag gtgcgcgtgt
     1441 gacactgctc aacctgcctg gcacaggagt ggccgattgc tccactgcag ggcctaggct
     1501 cccgtcgtgg acgtggtacg tgacggcgtc gtggatcttt tgggagtcct cacgtacgga
     1561 aagctgtccg cgttcgaccg gcttgccgag gattcccgtg ccgctcccac gctgactgcg
     1621 cggatcgcga tggccaccat ggcagctgct gagatcgggc acttccggct gctcgagcga
     1681 catctgcacg gcatgggcgt gtcggtggac gacgcggtgc gtccgttcgt ggcgcgcttc
     1741 gaccggttcc acgccttgac agcgccccga tcttggctgg agtcgctggt caaggtctac
     1801 gtcggggacg ggctggcctt cgacttctac ggtgaagtgg gtgagtggac cgacgacacg
     1861 accgccgcgc tggtcaagga ggtgttggcc gacaccgggc attctgcatt tgccgaacga
     1921 gaggtacggg cggcctgcga acggaaccgc accatccacg accggctcac cctgtggggc
     1981 cggcggttgc tgggcgaggc ggtgaccaat actcagcacg tcatggctga gcgggaggcc
     2041 cttgctgatc tgatcatccg tggctccggt gacgtgtccg gactcgctac gctgatccgc
     2101 cgggtgcaga tcctgcactg ccgccggatg gccgctctcg gactcgggtg agcagcggcg
     2161 cggtgttcga ctagccttga ccccaacttc gatcacactg gaggttctgc gtggagatca
     2221 agatcggcat cgcggacagc cctcgcgagc tgacggtgtc cactgcggac tccccagacc
     2281 agatagagac actggtcacc gaggcgctgc ggacaaccca aggattgctg acgctgatcg
     2341 acgaaaaggg ccgccggtac atggtgcccg cttcgcgggt cgcttacgtc gagatcgggc
     2401 ctccggacag ccgccgcgtc ggcttctcgg tgggtggctg accgccggtc gtgctgcgca
     2461 cgagttgcgt gcggggtgat ccattaggct catggagtgc cccggctgct cgtagtggag
     2521 gacgacgaga ccatcggcag cgtgctggag tccagcctgc gtgcacacgg gcaccaggtg
     2581 gcctggcagc gcaccggtcg tcgcgcgctg cgtctggccg ccgaagcgga gttcgacctg
     2641 gtgctgctgg acctggggtt gcccgatctg gatggcgtcg aggtgtgccg gcagttacgg
     2701 gccagcaatc ccacaacggt gctggtgatc ctcactgccc gaacggacga gatggacgtc
     2761 gtcgtaggcc tcgaggctgg tgcggacgac tacctgacca agccggtgcg gctggccgag
     2821 ctgctggcca gggtccgggc acacctgcgc cgcgtggcgc cggccacgcc gggcgctcca
     2881 ccgatgctca cggtcgcaga cctggtggtg gacaccggca gccggcgggt aaccgtcgca
     2941 ggacgggaag tgccgctgcg agccaaggag ttcgacctgc tcgcatgcct tgcggcagcg
     3001 cccgaggtgg cgctcagccg ggagacgctg atggcgcagg tgtgggacga gaactggttc
     3061 ggcccgacca agaccctcga tgtacacgtg gctgcgctgc gtcgcaagct ggccgggatg
     3121 gtcgagctcg gttcagtgcc gcggattgtg accctgcgcg ggcatgggta tcggctggag
     3181 gcgccggaga actgaacccg tggtcagcgc gaggtgttgt cggccgcctg aaatcccggc
     3241 cgggatggtc ggtcgatctc gaacggccgc atggaacccg ccatggtatt gccgatcaca
     3301 gagcagtcgg tggagcccat accgagctgc acggcgcggg agccggcccc ggaaaagtgg
     3361 ttgtcgcgca cgatgatgcg cgaaaagttt tccagcaaca ctgcctgtga accgttgacc
     3421 gaacaggtgt tgcgctcgaa gacgatcgcg gtatgccctt ccggggtgct gcgcgcaccg
     3481 ccctctttgg cggtggcgat caggcac
//
LOCUS       DATADN010000159         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_922869, whole genome shotgun sequence.
ACCESSION   DATADN010000159 DATADN010000000
VERSION     DATADN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922869"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VHH53_02115"
     CDS             <1..934
                     /locus_tag="VHH53_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2289002.1"
                     /translation="KRRVGWVYAVCDVDLDIREGETLGLVGESGCGKTTTLIEIMNLR
                     SPEHGGIRVGGVDVESLRGRGAERELRQRLQMVFQDPMGALDPRLTVFDILAEPLRAV
                     GEKDRSVIDNRVDELMELVGLDPEHSDRFPTAFSGGQRQRIGIARALATNPSLVVLDE
                     PVSALDVSIRAGVINLLNELKAKLGLSYLFVSHDLSVVRHISDRVAVMYLGRIVEVGA
                     VDQVFDHPQHPYTEALLSAIPVPDPQIERGRKRVILRGDLPSPTDSSPGCRFVSRCPL
                     HLTLNDAQRARCTDETPRLTGGGITDQRNACHFR"
     gene            1019..>2247
                     /locus_tag="VHH53_02120"
     CDS             1019..>2247
                     /locus_tag="VHH53_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HEX2289003.1"
                     /translation="MKRSIALAATAVLSVALVGCGSSGPADGNGDDTLDSLSEQAQHN
                     PQPYENIRDGGTLTTALGEINPQFNLWQGDTTLDTRTVWNWYNPLLITFTADGDAVYN
                     PDYLTDVRQETVDGNTRITYTINSRATYNDGSPIDWRSFEVVWKANNATNPAYIVASS
                     DGYDRIASVARGVDDRQAVVTFQGVNLWWQSLFNNLLHPNTLAPEVFNQGYINTPHPE
                     WGAGPYMIENFDRQAGTITFVRNPKWWGKPGKLDSRTFLTMESQASINAFRNGQLDAT
                     GVATQDRLAQVSGMSGIEIRKSIQPTLNFFTLNGRSPILADPVVRKAVYQGIDRQQLQ
                     EIHFQGLSFTAPLPGSMVILAFQDGYQDNFTKVITFNPEQAKQGLEAAGWVPGPDGVR
                     VKDGKPLEFTYVNTGDDP"
BASE COUNT          413 a          764 c          750 g          320 t
ORIGIN      
        1 gaaacgcagg gtgggttggg tgtacgcggt gtgcgacgtc gacctggaca tccgcgaggg
       61 cgagacactc gggttggtcg gcgagtcggg gtgcggcaag acaacgaccc tgatcgagat
      121 catgaacctg cgctcgccgg agcacggcgg catccgggtg ggcggcgtcg acgtcgagag
      181 cctgcgtggc cggggggcgg aacgcgagct gcgccagcgg ctgcagatgg tgttccagga
      241 cccgatgggc gcgctggatc cgcggctgac ggtcttcgac atcctggccg agccgctgcg
      301 ggcggtcggc gagaaggacc ggtcagtgat cgacaaccgg gtcgatgagc tgatggagct
      361 ggtcgggctc gatcccgagc acagcgaccg cttcccgacc gcgttctccg gcgggcagcg
      421 tcagcgcatc gggatcgccc gcgccctcgc caccaacccg tcgctggtcg tgctggacga
      481 accggtctcc gcgctggacg tctcgatccg ggccggcgtg atcaacctgc tcaacgagct
      541 gaaggccaag ctcgggctgt cctacctgtt cgtgtcccac gatctgtcag tggtgcggca
      601 catctccgac cgggtagcgg tgatgtacct ggggcggatc gtcgaggtcg gggcggtgga
      661 ccaggtgttc gaccaccccc agcacccgta caccgaggcc ctgctctcgg cgatcccggt
      721 gcccgacccg cagatcgagc gcgggcggaa acgggtgatc ctgcgtggtg acctgcccag
      781 ccccaccgac tcctcccccg gctgccggtt cgtcagccgc tgccccctgc acctgaccct
      841 caacgacgcg cagcgcgccc gctgcacgga cgagacgccc aggctgaccg gaggggggat
      901 caccgaccag cgcaacgcct gccacttccg ctgacgcgcg gcaccggccg tgcgacggcg
      961 cgcggcagac gcatttcccg ggaaggaacg gatgattaag gtcgtgaaga ggcccttcgt
     1021 gaagagaagc atcgccctcg cggcgaccgc ggtgctgtcg gtcgcgctgg tcggctgcgg
     1081 cagctcggga ccggccgacg ggaacggtga cgacaccctc gactcgctgt ccgagcaggc
     1141 ccagcacaac ccccagccct acgagaacat ccgggacggc gggacgctca ccaccgcact
     1201 gggggagatc aacccgcagt tcaacctctg gcagggcgac acaacgctgg acacccgcac
     1261 ggtgtggaac tggtacaacc cactgctgat cactttcacc gccgacgggg acgccgtcta
     1321 caaccccgac tacctgaccg acgtcaggca ggagacggtg gatggcaaca cccggatcac
     1381 ctacacaatc aactcgcggg ccacctacaa cgacggctcg ccgatcgact ggcgctcgtt
     1441 cgaggtcgtc tggaaggcca acaacgccac gaacccggcg tacatcgtcg cctccagcga
     1501 cggctacgac cgcatcgcct cggtggcccg gggcgtggac gaccggcagg cggtggtgac
     1561 cttccagggc gtcaacctgt ggtggcagag cctgttcaac aatctgctcc acccgaacac
     1621 gctggccccg gaggtgttca accagggcta catcaacacg ccgcacccgg aatggggcgc
     1681 aggcccgtac atgatcgaaa acttcgaccg gcaggcgggc acgatcacgt tcgtccgcaa
     1741 tccgaaatgg tggggcaagc cgggcaagct cgactcgcga acgttcctga ccatggagtc
     1801 ccaggcgtcg atcaacgcgt tcaggaacgg ccagcttgac gcgactggcg tggccaccca
     1861 ggaccggctg gcgcaggtga gcgggatgag cgggatcgag atccgcaaga gcatccagcc
     1921 gacgctgaac ttcttcaccc tcaacggcag gagtccgatc ctcgccgacc cggtggtgcg
     1981 caaggcggtg taccagggga tcgaccgcca gcaactacaa gagatccact tccagggcct
     2041 gagcttcacc gcgccgttgc ccggatcgat ggtcatcctg gccttccagg acggctacca
     2101 ggacaacttc accaaggtga tcaccttcaa cccggagcag gccaagcaag gcctcgaggc
     2161 ggcgggctgg gtgcctggtc cggacggggt ccgggtcaag gacggcaagc ccctggagtt
     2221 cacctacgtc aacaccggcg acgatcc
//
LOCUS       DATADN010000160         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_922883, whole genome shotgun sequence.
ACCESSION   DATADN010000160 DATADN010000000
VERSION     DATADN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922883"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VHH53_02125"
     CDS             <1..1111
                     /locus_tag="VHH53_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX2289004.1"
                     /translation="TIKQVEIEIIDRAWDEGWVVPQPPHIRTGKQVAVVGSGPAGLAA
                     AQQLTRVGHDVVVYERADRMGGLLRYGIPEFKMAKHRLNRRLEQMSAEGTEFRAGVDV
                     GVDVTAKELRGSFDAVVLAGGPTAWRDLPVPGRELTGIYQAMEYLPPSNRVQEGDLPR
                     SPIHAEGKRVVIIGGGDTGADCLGTAHRQGAASVTQLEIMPIPPEHRPDHQPWPTYPM
                     VYRVSSAHEEGGERLFSVSTQQFLGDADGRVRGLRLAEVVSEDGKFVPVPGSEQDLPA
                     DLVLLAMGFVGPEKGKLLEELGVDYDVRGNVARDLCFMTSVPGVFVAGDMGRGQSLIV
                     WAIAEGRSAAAGVDRYLTGRDVLPAAIAPTERPLA"
     gene            complement(1161..1484)
                     /locus_tag="VHH53_02130"
     CDS             complement(1161..1484)
                     /locus_tag="VHH53_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX2289005.1"
                     /translation="MPPESQDLYAILKVAPDASQTQIGRAYRRLIRAHHPDTHPDHDS
                     AALAAAVAAYAILRDPARRAAYDQARRPASNQHPTAPPPATSALHRRRPEPDIRAGPV
                     RWYPK"
     gene            complement(1531..>1721)
                     /locus_tag="VHH53_02135"
     CDS             complement(1531..>1721)
                     /locus_tag="VHH53_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289006.1"
                     /translation="RYVQITAACHRNPVHGRVTAVLTADEHYTFWVALLPDGDLAAGD
                     ERIVALSTLAAAWSATEP"
BASE COUNT          265 a          520 c          648 g          288 t
ORIGIN      
        1 gaccatcaag caggtcgaga tcgagatcat tgaccgggct tgggatgagg gttgggttgt
       61 cccacaacct ccgcatatcc gcaccggtaa gcaggtcgcc gtagtcggat ccggcccagc
      121 tgggctggct gcggcccagc agctgacccg ggtcgggcac gacgtggtgg tctacgagcg
      181 cgcggaccgg atgggtgggc tgctgcgcta cggcatccct gaattcaaga tggccaagca
      241 ccggttgaac cggcgactgg agcagatgtc cgcggagggc accgagttcc gggccggcgt
      301 cgacgtcggc gtcgacgtca cagctaagga gctgcgcggg agcttcgatg ctgtggtgct
      361 cgcaggtggt cctaccgcgt ggcgggacct gccggttcct ggacgcgagc taaccggtat
      421 ttaccaggcg atggagtacc tgccgccgtc caaccgggta caggagggtg acctgccgcg
      481 gtcgccgatc cacgccgaag gcaaacgtgt ggtgatcatc ggtggggggg acaccggagc
      541 cgactgcctg ggcaccgcgc atcgccaggg cgctgcctcc gtgacccagc tggaaatcat
      601 gccgattccg ccggagcacc gccctgatca ccagccttgg ccgacgtacc cgatggtcta
      661 ccgggtttcc tcggcccacg aggagggtgg tgagcggctg ttctcggtga gcacccagca
      721 gttcctcggt gacgccgacg gccgggtgcg cgggctacgg ctggccgagg tggtctccga
      781 ggacggcaag ttcgtgccgg taccaggcag cgagcaggac ctgcccgccg acctggtact
      841 gctggcgatg gggttcgtcg ggcccgagaa gggcaagctg ctggaggagc tgggtgtcga
      901 ctacgacgtc cgcggcaacg tcgctcgcga cctgtgtttc atgaccagcg tgccgggcgt
      961 gttcgtcgcc ggagacatgg gtcgcggtca gtcgctgatc gtatgggcca tcgccgaggg
     1021 ccgctctgct gcggccggtg tggaccggta cctcaccggc cgcgacgtac taccggccgc
     1081 gatcgcgccc accgagcggc cgcttgcctg acggtccccg agtggctctc taggcgccgg
     1141 ccagccgcgt tccgcagcac ctacttggga taccaccgga ctggaccggc acggatatcg
     1201 ggttccgggc ggcgacggtg cagtgccgaa gttgccggcg gtggtgcggt ggggtgctgg
     1261 ttgctggcgg gacgccgggc ctggtcgtag gctgctcggc gagcaggatc acgcaggatg
     1321 gcgtaggccg ccacagcggc agctagcgca gcagagtcat gatcgggatg tgtgtcgggg
     1381 tggtgggcgc ggatcaggcg ccggtaggcg cgaccgatct gcgtctgcga ggcatccggg
     1441 gccactttca agatggcata gagatcctga gattcaggtg gcatcgcagt tgtccatccg
     1501 ggtgtccctg ggccgacggc gggcgttgag ttatggctcg gtggctgacc aggcggcggc
     1561 cagggtgctc agtgcaacga tgcgctcgtc accggcggcc aagtccccat cgggcaggag
     1621 cgcgacccag aacgtgtaat gctcgtcagc agtgagcaca gcggtgaccc ggccgtggac
     1681 ggggttgcgg tgacaagcgg cggtgatctg gacgtagcgt t
//
LOCUS       DATADN010000161         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_319780, whole genome shotgun sequence.
ACCESSION   DATADN010000161 DATADN010000000
VERSION     DATADN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319780"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VHH53_02140"
     CDS             complement(<1..1148)
                     /locus_tag="VHH53_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2289007.1"
                     /translation="MTLTSGQLLGRRYRLSRRIAIGGMGEVWEAADTRLERRVAVKVL
                     KPELSGDAEFRYRFHTEAMMTASLNHPGIAAVHDYGEAGNDPESAYPWAYLVMELIAG
                     DPLATILMGHSRLPVDRTLDVLGQAAAALQAAHERGLVHRDIKPGNILITAAGTVKLT
                     DFGIARAVDAAPVTRHGMVMGTAHYIAPEQAAGHEAGPPSDVYSLAVVGYECLAGRRP
                     FLADNAVTVAMMHIHDRPPPLPPDVPAGVRALIEAALAKDPQQRYGSGGEFAAAVAAV
                     RAGHRLPLPVGKTATSPAHTSMVDKAGTAPPPTALLPAGSPTGPYPGYQPRQRFDRRA
                     TARIAMGLLGVLALGLSGYLVNDALRSDATPPQAAGIGTAPQAPVAPNP"
     gene            complement(1174..2649)
                     /locus_tag="VHH53_02145"
     CDS             complement(1174..2649)
                     /locus_tag="VHH53_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2289008.1"
                     /translation="MNTPLRRVALAVMGMIVLLLANATYIQVVSADTYRSDPRNQRVL
                     LDEYSRQRGQIVAGGLALASSVETGRQLRFQRTYLDGPLYAPVTGYYSLRYGAGGIEN
                     AMDSVLNGSDDRLFVRRLSDLITGRTPSGGSVELTINPIVQRMAYEQLTRRGYSGAVV
                     AVKPSTGEILAMASTPSYDPNRLSAHGGAEQETAWNAFTSPERGSPLTNRAVGATYPP
                     GSTFKLVVTAAALETDRATPDTPLTATAALQLQDTSTLLRNFADNSCGRGVTATLQEA
                     LVRSCNTAFAQLVATVGENDLRKQAQLLGIGQQGLTIPILVTPSTLGDIPDVAALQQS
                     GIGQRDVALSPLQNAMIAATIANGGRRMKPQMVRNILAPDLSVLGSFEPEQVETAMSR
                     ITASQLRDMMVAAENTYSNDGKISGVGIAAKTGTAEHGVDPKNTPPHAWYVAFAPANN
                     PQVAIAVVVEDGGDRDLEATGGSRAAPIGRAVIGAALQGGR"
     gene            complement(2646..>2928)
                     /locus_tag="VHH53_02150"
     CDS             complement(2646..>2928)
                     /locus_tag="VHH53_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2289009.1"
                     /translation="VRDTFGKLLAAGLSFAVGLQVFVVVGGVTKLIPLTGLTAPFLSY
                     GGSSLLANYILVALLLRISHAARSPAAPPVPRKPVAPLAEARTEMVQRP"
BASE COUNT          468 a          957 c         1025 g          478 t
ORIGIN      
        1 gggttgggtg ctacgggcgc ttgcggggcc gtgccgatgc cggctgcttg ggggggtgtg
       61 gcgtcagaac gcagcgcatc gttgaccaga tagccactca gccccagcgc gagcactccg
      121 agtagcccca tcgcaattct cgctgtggcg cgtcgatcga agcgctgccg cggctggtac
      181 ccggggtaag ggccggtggg tgaccctgcc ggcagcagtg cagtcggtgg cggtgccgtg
      241 ccggctttgt cgaccatgct ggtgtgagca ggactcgtgg cggtctttcc caccggcagt
      301 ggcaggcggt ggccggctcg gaccgcggcg acggcagcgg cgaactcacc gccggagccg
      361 taacgctgct gcggatcctt ggcaagcgcc gcctcgatca gcgcacgaac cccagcgggc
      421 acatccggcg gcagcggggg cggccggtcg tggatatgca tcatcgcgac ggtgaccgcg
      481 ttgtcggcca ggaacgggcg ccggccggcg aggcactcgt aacccaccac ggccagcgag
      541 taaacgtcgc tgggcggccc ggcctcgtgc ccggccgcct gctccggagc gatgtagtgg
      601 gcagtgccca tcaccatccc gtgccgggtc accggagctg cgtcgacggc cctggcgatg
      661 ccgaagtcgg tgagtttcac cgttccggca gcagtgatca ggatgttgcc cggcttgatg
      721 tcgcggtgta ccagaccacg ctcgtgcgcc gcctgcagcg cggcggcggc ctgcccgaga
      781 acatccaacg tgcggtcgac gggcaaccgc gaatgcccca tgaggatcgt cgcgagaggg
      841 tctccagcga tcagctccat caccagatag gcccaaggat aggcagactc tggatcgttg
      901 cccgcttcac cgtagtcgtg caccgcagcg atgccgggat ggttcagcga cgcggtcatc
      961 atggcctcgg tgtggaagcg gtaccggaat tctgcgtcgc cggaaagctc cggtttgagt
     1021 accttgacgg cgacccgacg ctccagccgg gtatctgcag cctcccagac ctcgcccatc
     1081 ccgccgatgg cgatacgccg gctcagccgg tagcgccgcc ccagcagctg gccgctggtc
     1141 aaagtcataa gcgcaccctg ttgcgtacag tcatcagcga ccgccctgca gagcagcccc
     1201 gatgacggct cggccgatcg gcgcggcccg ggacccaccg gttgcctcaa gatcccggtc
     1261 tcccccgtct tcgaccacca cagcgatcgc tacctgcgga ttgttggcgg gcgcgaaggc
     1321 cacataccag gcgtgcggcg gggtgttctt ggggtcgaca ccgtgttcgg cggtgccggt
     1381 cttcgcagcg ataccgacgc cgctgatttt accgtcgtta ctgtaagtgt tttcggcggc
     1441 gaccatcatg tcgcgcagct gggatgcagt aatgcgagac atcgccgttt cgacttgctc
     1501 tggttcgaag ctgcccagta ctgagagatc aggggccaag atgttacgaa ccatctgggg
     1561 tttcatccgt cgccctccgt tggcaatggt cgcggcgatc atcgcattct gcaacgggct
     1621 gagcgctaca tcacgctggc cgatgccgga ctgttgcaag gcggcaacgt cgggaatgtc
     1681 acccagagtg gacggcgtta ccaggatcgg aatggtgaga ccctgctgcc cgatgccgag
     1741 cagttgagcc tgcttgcgca ggtcgttctc acccacggta gcgaccagct gggcgaaggc
     1801 ggtgttgcag gagcgcacca gcgcctcttg gagagtcgcg gtgacgccgc ggccacaaga
     1861 attgtcggcg aagttgcgca gcaacgtcga ggtgtcctgc agctgcaagg cagccgtcgc
     1921 ggtcagcggt gtgtccgggg ttgccctatc ggtctccagc gcagccgcgg tgaccacgag
     1981 cttgaaggtc gatcccggcg ggtaggtcgc cccgaccgcc cggttggtca gcggtgagcc
     2041 acgctccggc gaggtgaacg cgttccaggc agtctcctgc tccgcgccgc cgtgcgcaga
     2101 cagccggttc gggtcgtagg agggggtgga ggccatcgcc aggatctcgc cggtactggg
     2161 cttgaccgcc accaccgcgc cgctgtagcc acggcgggtc agctgctcat aggccatccg
     2221 ctggacgatc gggttgatgg tgagttcgac gctgcccccg ctgggcgtcc gcccggtgat
     2281 caggtccgaa agccgccgca cgaacagccg gtcatcggag ccgttgagca ccgagtccat
     2341 cgcgttctcg atgccaccgg cgccgtagcg cagcgagtag tacccggtga cgggggcgta
     2401 cagcgggccg tcgaggtagg tgcgctggaa gcgcagctgc ctgccggtct ccaccgagct
     2461 ggccagcgcg agcccgccgg cgacgatctg cccgcgctgc ctgctgtact catcgagcaa
     2521 cacccgctgg ttgcgtgggt cgctgcggta ggtgtcagca ctgacgacct ggatgtaggt
     2581 ggcgttggcc agcagcaaaa cgatcatgcc catgacggcc agcgcgaccc ggcgaagcgg
     2641 tgtgttcacg gccgctgcac catctcggtg cgcgcctcgg ccagcggggc taccggtttg
     2701 cgtggtaccg gcggggccgc cggcgaccgc gccgcgtggg agatccgcaa cagcagggcc
     2761 accaggatgt agttggccag cagcgacgac ccgccgtagg acaggaacgg cgcggtgagc
     2821 ccggtcagcg ggatcagctt ggtcaccccg ccgacgacga cgaacacctg caggccgacc
     2881 gcgaaagaca gcccggcggc gagcagcttg ccgaaggtgt cccggacg
//
LOCUS       DATADN010000162         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_16582, whole genome shotgun sequence.
ACCESSION   DATADN010000162 DATADN010000000
VERSION     DATADN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16582"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(486..1400)
                     /locus_tag="VHH53_02155"
     CDS             complement(486..1400)
                     /locus_tag="VHH53_02155"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEX2289010.1"
                     /translation="MLAAVSVSELLDPAAWRRVEGFNFADITYHRAVAHGTVRIAFDR
                     PEVRNAFRPSTVDELYQALDHARMTPDVGCVLLTGNGPSPRDGGWAFCSGGDQRIRGR
                     SGYQYASGETADTIDSARAGRLHILECQRLIRFMPKVVIAVVPGWAAGGGHSLHVVCD
                     LTLASAEHARFKQTDADVGSFDGGFGSAYLARQVGQKFAREIFFLGREYSAADAHRMG
                     MVNAVVPHASLEATALQWAGAVNAKSPTAQRMLKYAFNLIDDGLVGQQLFAGESTRLA
                     YMTDEAVEGRDSFLEKRPPDWSAFPWYY"
     gene            1440..>2107
                     /locus_tag="VHH53_02160"
     CDS             1440..>2107
                     /locus_tag="VHH53_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX2289011.1"
                     /translation="MNPSTALATVLVDELVRCGVRHAVLCPGSRNAPLSFALHAAEQA
                     GRLTLHVRIDERSAAFLALGMSLRSGMSVPVVCTSGTAAANLHPAMLEAHHAGVALFA
                     VTADRPAELVDTGANQTVHQTGLFGVAVRAAPSLSLGQPAQWRSAVDRAVAAACGSLG
                     GAPGPVHLNVPLREPLMPEPGSQWPVAPAGRAGDRPWTAVAAGSPACVEVELDLSRRT
                     LVIAG"
BASE COUNT          346 a          733 c          696 g          332 t
ORIGIN      
        1 acagggacat gatcaccttc tgctgtgctg ccgtggctca cggcagcacg agctgctcca
       61 cccaggccgt cccgaaatga atgcccagca gcagtgccaa cggcaggacg acggcgacca
      121 acgtgagcag aagcattgcc ctggtccaca tggctcggcc ccgccttcac ccggttaccc
      181 ctcctcggca ccatgttcat cgctgcgagc tggccggggg ttaacagcga tcgctcgagg
      241 tcatggttgc cgccgtcatc tttcgctcac caacgggcct ggcgtgggcg tcatctccac
      301 tcgtgtatag ccgctggacc gggtgccact ggcattgcag cgcggaaccc ggctggccca
      361 tcccctcgcg ccgcacaggc gccggtacga gggcgtacta tgccgcccaa cccataaccc
      421 acgcgaggcg gacgccgcac cgcgggcgcc gcaacgataa gcagggactg ggggaataac
      481 ccctactagt agtaccaggg aaatgccgac cagtccggag ggcgcttctc caagaaggaa
      541 tcgcggccct cgacggcctc gtcagtcatg tacgctagcc gggtcgattc accggcgaac
      601 agttgctggc cgaccagccc gtcatcgatc aggttgaacg cgtatttgag catccgctgc
      661 gcggtggggg acttggcgtt gaccgcgccg gcccactgca gtgccgttgc ctccagcgat
      721 gcgtgcggta ccaccgcgtt gaccataccc atccggtgcg cgtcggccgc gctgtactcc
      781 cgcccgagga aaaagatctc ccgggcgaac ttctgcccga cttgccgggc gagataggcc
      841 gagccgaatc cgccgtcgaa agatcccaca tcagcgtcgg tctgcttgaa cctagcgtgc
      901 tcggccgacg ccagggtcag gtcacaaacc acgtgcaagg aatgcccacc accggctgcc
      961 cagcccggta caaccgcgat caccaccttg ggcatgaagc ggatcaatcg ttgacactcc
     1021 aagatgtgca gccgcccagc ccgcgccgaa tcgatggtgt ccgccgtctc acctgatgcg
     1081 tactggtacc ccgaccggcc tcgaatccgc tggtcgccac ccgagcagaa ggcccagccg
     1141 ccatcgcggg gtgagggccc gttaccggtc agcagcacgc atcccacgtc cggcgtcatc
     1201 cgggcgtgat ccagcgcctg gtacagctca tccaccgtgg acggacggaa ggcattgcgg
     1261 acctcgggcc ggtcgaaggc gatccggacc gtgccgtgcg ccactgcgcg gtggtaggtg
     1321 atgtcggcga agttgaagcc ctcgacacgg cgccaggcgg caggatcaag tagctcggag
     1381 acgctcacgg cggcgagcat acgagcagcg cgcaccccgg cacggcatgc tgaccactcg
     1441 tgaacccgtc cactgcattg gccaccgtgc tggtcgacga gctggtgcgg tgcggcgtgc
     1501 ggcacgcggt gctctgcccg gggtcgcgca atgcgccact gtccttcgcc ctgcatgcgg
     1561 ccgagcaggc tggacggctg accctgcatg ttcgcatcga cgagcgctcc gcggcgttcc
     1621 tggcgctggg catgtcgctg cgctccggta tgtcggtacc ggtggtgtgc acctcgggca
     1681 ctgcggccgc gaacctgcac ccagcgatgc tggaagcaca ccacgccggg gttgcgctgt
     1741 tcgcggtgac cgccgaccgc ccggccgagc tggtcgacac cggagccaac cagacggtgc
     1801 accaaaccgg cctgttcggt gtagcggtac gtgcggcacc gtcgctgtca cttggtcagc
     1861 cggcacagtg gcgctccgca gtcgaccgtg cggtggcggc cgcgtgcggg tcgctgggtg
     1921 gcgctccggg cccggtacac ctcaacgtgc cgctgcgcga accgctgatg cccgagcctg
     1981 ggtcgcagtg gccagtggcg ccggccgggc gtgcgggtga tcgaccctgg actgcggttg
     2041 cggcgggctc accggcctgc gttgaagtgg agctggacct gagccggcgc acgctcgtga
     2101 tcgccgg
//
LOCUS       DATADN010000163         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_168808, whole genome shotgun sequence.
ACCESSION   DATADN010000163 DATADN010000000
VERSION     DATADN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168808"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VHH53_02165"
     CDS             complement(<1..1638)
                     /locus_tag="VHH53_02165"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289012.1"
                     /translation="MPFSIQRLRGRGLVAEIQATDLAFDLANVDALLRKTVGRDPPGL
                     SKRVWEQTGGWPAAVHCAVEMLRGVGPDQRLAAVRQLSLPGQRFHDYLAEEVIGTSAQ
                     GVQQLLRRLAIFGEVSSTMEIAPGHDDPAIALADLSRQGFVLRTGGGDSWSLVRPLRD
                     FFEHEPVPSASERKALHVTAAKECIERGASAEALRHLLAADDHAACASLLVDHGGTMV
                     ERGEVDNVLEAAELPDQYLGDPRIQRVLGQAQQVRGRWAQALQYFQHAGHDRDELEPA
                     LAWRVGVIALARGEFNEVHALIRRTRLGQEDTLDETRVLALAASAHRMTGDLVNLRKV
                     ALRARAAARRCGEPRAWSSVHHIFALLAAAEGDWRQADAHCTEAMHSAEVSDDLLGLM
                     WIRACRAFHQFELGAPRQALAETQIVLSLSQRCEDPFLIAYALTIRGRARGRLGMLEA
                     AAADFTTAIGLFQRLGSRFLAWPLCGLGDLHRTKGQLNRAQAAYEEALTLAEPCHDVF
                     GLSSALIGLARTTVADDLKRARERADRAVELDEGLRTV"
BASE COUNT          329 a          625 c          495 g          279 t
ORIGIN      
        1 gaccgtgcga agtccttcgt ccaactccac ggcgcggtct gcgcgctcac gagctcgctt
       61 gaggtcatca gccacagtgg tgcgtgccag accgatcagc gccgaactga gaccgaacac
      121 gtcgtggcag ggctcggcga gggttagggc ctcctcgtac gcggcctggg ctcggtttag
      181 ttggcccttg gtccgatgca gatcacccag gccacacagt ggccaggcca gaaatcgaga
      241 accgaggcgc tggaaaaggc cgatcgctgt ggtgaagtcg gctgccgcgg cttccagcat
      301 ccccaggcgc ccacgggcgc ggccgcgaat ggtcagcgcg tacgcgatga ggaacgggtc
      361 ctcgcatcgc tggctgaggc tcaacacgat ctgggtctca gccagcgcct gccgtggtgc
      421 ccccaactcg aactggtgaa atgcccggca agctcgaatc cacatcaacc caagcaggtc
      481 gtcgctcacc tcggcgctgt gcatcgcctc ggtgcagtgc gcgtcggctt gccgccagtc
      541 accctctgca gcggccagca gcgcgaagat gtgatggaca ctggaccacg cacgtggctc
      601 accgcaccgg cgagccgcag cacgtgctcg aagcgcaact ttccgtagat tcaccaggtc
      661 accggtcata cggtgggcac tggccgccag cgccagcacc cgcgtctcgt ccagcgtgtc
      721 ctcttggccc aaccgggttc gccggatcag cgcgtgcacc tcattgaact cgccccgagc
      781 caaagcgatc acaccgactc gccaggccag cgccggctct aattcgtctc gatcatgtcc
      841 agcgtgctga aaatactgca gcgcctgcgc ccatcggccc cgtacctgct gcgcctgccc
      901 caaaactcgt tggatccgcg ggtcgccgag gtactggtcc ggtaactcgg cagcctccaa
      961 aacgttgtcg acttcgccgc gctccaccat cgtcccacca tgatcaacca gcagtgaggc
     1021 gcacgccgcg tgatcatccg ctgcgaggag atgccgcagg gcctcagctg atgctccccg
     1081 ctcgatacac tctttagccg ccgtcacgtg cagcgctttg cgctcgcttg cagacggcac
     1141 cggctcatgc tcgaaaaagt cacgtaatgg acgcacaagc gaccagctgt cacccccacc
     1201 ggtgcgcagc acaaaaccct ggcgactcag atcggccagc gcgatcgccg gatcgtcgtg
     1261 cccaggcgcg atctccatcg tcgagctgac ctcgccgaag atcgccaacc tgcgcagcag
     1321 ctgctggacc ccttgagccg aggtgccgat gacctcctca gccagataat catgaaatcg
     1381 ttgtcccgga agggacaact gccgcactgc tgccaggcgc tggtccggcc cgactccccg
     1441 caacatctct accgcgcagt gcaccgccgc gggccacccg ccagtctgtt cccacacccg
     1501 cttcgacaag ccaggcggat cccttcccac ggtcttgcgc agcagcgcat cgacattggc
     1561 gaggtcgaag gcaaggtccg tcgcctgaat ctccgcgaca agaccgcgac ctcgcaaccg
     1621 ctgaatggag aacggcaact cacatcggga tatcaatacc agatgcaacc gatccgatat
     1681 gcgccggcac aaaccctcca ctacggccgc cgcgtcactc cccggctg
//
LOCUS       DATADN010000164         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1073868, whole genome shotgun sequence.
ACCESSION   DATADN010000164 DATADN010000000
VERSION     DATADN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073868"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..691
                     /gene="rplD"
                     /locus_tag="VHH53_02170"
     CDS             23..691
                     /gene="rplD"
                     /locus_tag="VHH53_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX2289013.1"
                     /translation="MHNPEGGITGTAELPDEVFDVPANIPLMHQVVVAQLAAARQGTH
                     DTKTRGEVSGGGSKPYRQKGTGRARQGSIRAPQFTGGGIVHGPTPRDYTQRTPKKMKV
                     AALRGALSDRARDGRVHVISGLVSGDVPSTKTARKVLEGLVEQGNRLLVVLSRADEVA
                     RRSVRNLPSVHVLAPDQLNTYDVLINDDVVFTADALEAFLARSVRGRSATTVGAPLME
                     EDGR"
     gene            688..987
                     /gene="rplW"
                     /locus_tag="VHH53_02175"
     CDS             688..987
                     /gene="rplW"
                     /locus_tag="VHH53_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX2289014.1"
                     /translation="MIPDPRDVILKPVISEKSYGLLEKSKYTFVVAPDANKTEIKIAV
                     QKIFGVQVVSVNTLNRSGKRKRTRFGYGKRKDTKRAIVTLSAESKPIEIFGGPAA"
     gene            1016..1795
                     /gene="rplB"
                     /locus_tag="VHH53_02180"
                     /pseudo
     CDS             1016..1795
                     /gene="rplB"
                     /locus_tag="VHH53_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            complement(1801..1933)
                     /locus_tag="VHH53_02185"
                     /pseudo
     CDS             complement(1801..1933)
                     /locus_tag="VHH53_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L2"
BASE COUNT          365 a          634 c          655 g          279 t
ORIGIN      
        1 cccagaaacg aaaacggttg acatccacaa tcccgaaggc ggcatcaccg gcaccgccga
       61 attgcccgac gaggtattcg acgtgccggc gaacatcccc ctgatgcatc aggtggtggt
      121 cgcccagctg gccgcggcac ggcaaggcac gcacgacacc aagacccggg gtgaggtcag
      181 cggcgggggc agcaagccct accggcagaa gggcaccggg cgtgcccggc agggctcgat
      241 cagggcgccg cagttcaccg gcggcggcat cgtgcacggc cccacgccac gggactacac
      301 ccagcgcacc cccaagaaga tgaaggttgc cgcgctacgt ggtgcgctgt cggatcgggc
      361 tcgcgacgga cgggtgcacg tgatctctgg attggtctcc ggtgatgtcc cgtcgaccaa
      421 gacggctcgt aaagtcctcg aagggcttgt cgagcagggt aaccggttgc tggtggtgct
      481 cagccgcgcc gacgaggtgg cacgtcgcag cgtgcgaaat ctcccttcgg tgcacgtgct
      541 ggctcccgat cagctcaaca cctacgacgt cctgatcaac gatgacgtcg tgttcaccgc
      601 cgatgcgctg gaggcattcc tggctcgctc ggtgcggggg cgttcggcca ccacggtggg
      661 ggctccgctg atggaggagg atggtcgatg atccccgacc ctcgtgacgt gatcctcaag
      721 ccggtcatct cggagaagag ctacgggctg ctggagaaga gcaagtacac cttcgtcgtc
      781 gcgccggacg ccaataagac cgagatcaag attgccgtgc agaagatctt cggcgtacag
      841 gtggtcagcg tcaacacgct caaccgttcg ggcaagcgca agcggacccg gttcgggtac
      901 ggcaagcgca aagacaccaa gcgggcgatc gtgacgctgt ccgctgagag caagccgatc
      961 gagatcttcg gcggaccggc cgcctgagcg gtcaccaaag gaactgggct accacatggg
     1021 catccgcaag tacaagccga cgacctcggg tcgccgcggc tccagtgtct ccgacttcgc
     1081 cgagatcacc cgagaccacc cggagaagtc gctggtccgt cccctgcacg gccgcggcgg
     1141 gcgcaacgtc cacggccgga tcacgacccg gcaccagggt ggcgggcaca agcgcgctta
     1201 ccgcctgatc gatttccggc gcgcggacaa ggacggcgtg ccggccaagg ttgcgcacat
     1261 cgagtacgac ccgaaccgca ctgcccggat cgcactgctg cactacgccg acggcgagaa
     1321 gcgatacatc atcgccccgg agaagctgcg ccagggtgac atgttggaga acggctcgat
     1381 ggccgacatc aagccgggca acaacctgcc gctgcgcaac atcccggtcg gcacggtggt
     1441 gcacgcgatc gagctgcgtc ccggtggcgg cgcgaagatc gcccgctcgg ctggcgcacg
     1501 ggttcagttg gtggccaagg acggtcccta cggccagctg cggatgccgt ccggcgaaat
     1561 ccgcaatgtc gatgtccggt gtcgcgccac ggtgggtgag gtcggaaact ccgaacagag
     1621 caacatcaac tggggcaagg ccggtcggat gcgctggaag ggtaagcgac ccacggttcg
     1681 cggtgtggtg atgaacccag tcgatcaccc gcacggtggc ggtgagggca agacctccgg
     1741 cggccggcac ccggtaaacc cgaagggcaa gcccgagggc cgcacccgcc gcaagggcaa
     1801 gccctcaccg ccaccgtgcg ggtggtcgac cgggttcatg gccacgccgc ggacggtcgg
     1861 gcgcttgccc ttccaccgca tccggccggc cttgccccag ttgatgttgg agtgctcggc
     1921 gttgccgacc tcg
//
LOCUS       DATADN010000165         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1220345, whole genome shotgun sequence.
ACCESSION   DATADN010000165 DATADN010000000
VERSION     DATADN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220345"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1073
                     /locus_tag="VHH53_02190"
     CDS             90..1073
                     /locus_tag="VHH53_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5914 domain-containing protein"
                     /protein_id="HEX2289015.1"
                     /translation="MFERGVPVRRLPAWTWAGQQPTWREARPGLIEAALKRALARPSG
                     NWYVLAASREVRDDRPFGRTVAGVEVVAWRTPDGALHAGPGECPHLGAPLCRGAVTGG
                     ELVCRWHGLAIGARGGPGWDPFPVYDDGVLAWVRLDAVGGEEPLDEPVLARRPDPETS
                     LDAVFTGIGRCEPEDVIANRLDPWHGSWFHPYSFVGLRVVEEPGNDVDRFVVEVAFRL
                     TRRFGVPVRAAFSCPEPRTIVMDILEGEGAGSIVETHAVPLGVDDAGHPRTAVIEATI
                     ASSPRPGFAVARRGAPVMRPAMRWAAARLWRDDLAYAQRRYDLRTTGRWPG"
     gene            complement(1169..2668)
                     /locus_tag="VHH53_02195"
     CDS             complement(1169..2668)
                     /locus_tag="VHH53_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015580020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2289016.1"
                     /translation="MLHPPRDGRLRPEGQPPKVAVVGGGIAGIAAAVALAERGVQVQL
                     IEREQQLGGRLRGWPTTLADGSSVTMSRGFHAFFRQYYNLRALLRRADPLGHALTPLS
                     DYPLIDADGHRDTFAGLPRTPPWNALAFALTSPTFRLRDLATRINPVAALPLIDVSVP
                     EVYWRLDNQDAMTFLERVRFPDAARHLAFSVFSRSFFADPRELSAAELATMFHIYFLG
                     SAEGLVFDVTAEPFPQALWEPMGDYLAELGVVVRTGVAVKEVAPGLDRRFALHAGGDS
                     DEVDAVVLAADVRGLQELITTSPELGERCWRERIGRLRTAPPFLVSRLWLDRPVDPGR
                     SGFLGTSGFGPLDNVSVLERFEGEAARWVRRTGGSVVELHAYALTGNSAQGKRQLVDE
                     LHRIYPETAAANIVDERHELQADCPLFAPGTFTDRPTVTTPDPAVVLAGDLTRVDLPV
                     ALMERAATSGLLAANALLGRWGLRGHPLWTVPVRGRLAPLRAAAARFAG"
     gene            complement(2716..3417)
                     /locus_tag="VHH53_02200"
     CDS             complement(2716..3417)
                     /locus_tag="VHH53_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX2289017.1"
                     /translation="MTIDVPSEFDAAAANYDRLVAASPGYHDNLARSAHRMQLPGQGA
                     GLHLLDLGCGTGASTAALLAAAPQATITAVDASAGMLARARQADWPQRVSFVHSRAED
                     LAAAGVHGPFDGIFAAYLVRNLPDPDPVLARLRDLLAPGAPLAIHDYALDGRVRSRAI
                     WTAVCWTVIIPAGKVVTGRASLYRYLWRSVLHFDTVDALAQRLRRHGLDEAGIATMPG
                     WERGIVHTVVARRSQ"
     gene            complement(3427..>3753)
                     /locus_tag="VHH53_02205"
     CDS             complement(3427..>3753)
                     /locus_tag="VHH53_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289018.1"
                     /translation="LRFHHTNHESLLAYSKTDPATDDVIVCVVTLEPYITVSGTVSLD
                     LPALGMRWQDRFLVHDEVSGETYDWGQTNYVKLQPWRAVAHILRLGRLRVTTPAIAGI
                     GAHGQP"
BASE COUNT          586 a         1266 c         1348 g          553 t
ORIGIN      
        1 agtggaaccg atgagcgggt tcggaagccg agccggccgc cccgtggaac cgaatggtgg
       61 ttcgggtgtg gccggaagga tgtggggcgg tgttcgaacg gggcgtgccg gtacggcggc
      121 tgccggcctg gacttgggcc gggcagcagc caacctggcg ggaggcccgg cccggtttga
      181 tcgaggcagc gctcaaacgg gcgctggccc ggccgtcggg caactggtac gtgctcgcgg
      241 caagccggga ggtccgcgac gaccggccct tcgggcgcac cgttgcgggg gtcgaagtgg
      301 tggcctggcg caccccggac ggtgcactgc acgccggtcc cggtgagtgc ccgcacctgg
      361 gggcaccact gtgccgcggg gcggtgaccg gcggcgagct ggtgtgtcgg tggcacggtc
      421 tggcaatcgg agcccggggt ggcccgggat gggatccgtt tccggtgtac gacgatggcg
      481 tgctggcttg ggtgcggctg gatgccgtag gcggggagga gccgcttgac gagccggtgc
      541 tcgcgcggcg gcctgatccg gagaccagcc tggacgcggt gttcaccggg atcggtcgct
      601 gcgagcccga ggacgtgatc gccaaccggc tcgacccgtg gcacggcagc tggtttcacc
      661 cgtactcctt cgtggggctc cgcgtggttg aggagcccgg caatgatgtg gatcggttcg
      721 tggtggaggt tgccttccgg ctcactcgac gcttcggggt tccggtgcgg gccgcgttca
      781 gttgccccga accgcggacg atcgtgatgg acatcctgga gggggaaggc gctggcagca
      841 tcgtcgagac ccacgccgtg ccgttaggtg tcgacgatgc cggtcatcca cgcaccgccg
      901 tgatcgaggc gaccattgcc agctcacctc gcccgggatt cgccgtcgcc cgccgcgggg
      961 caccggtgat gcgccctgcc atgcgctggg ccgccgcccg cctatggcgc gacgacctcg
     1021 cctacgccca acgccgctac gacctccgca ccaccggccg ctggccaggc tgaactcagg
     1081 ctgcgttctg gagacagatt gctgtcatca ggcacggaat taccgcagtc tgtccccaga
     1141 acgcgagagc tggctggttt gctgatgctc acccagcgaa gcgggccgcg gcggcgcgca
     1201 gtggggccag ccggccgcgg acagggactg tccacagtgg atgcccgcgc agtccccacc
     1261 tgcccagcag tgcgttggcg gccaacaacc cgctggtcgc ggcgcgttcc atcagcgcga
     1321 cggggagatc tacccgggta aggtcgccag cgagcaccac ggccgggtcg ggagtggtga
     1381 cggtgggccg gtcggtgaac gttccgggcg cgaacagtgg gcagtcggcc tgcagctcgt
     1441 ggcgctcgtc gacgatgttg gcggcggcgg tctccgggta gatccggtgc agctcgtcga
     1501 ccagctggcg cttgccctgc gccgaattgc cggtcagcgc gtaagcgtgc agttccacca
     1561 ctgagccccc tgtgcggcgc acccaccggg ccgcctcgcc ttcgaatcgc tccagcacgc
     1621 tgacgttgtc cagcggcccg aagccgctgg tgcccaggaa cccggatcgg ccgggatcga
     1681 ccggacggtc cagccacaac cgggagacca ggaacggcgg cgcggtgcgc agcctcccga
     1741 tgcgttcccg ccaacatcgt tcgccaagct caggagaagt ggtgatcagc tcctgcaacc
     1801 cgcgcacgtc ggcggcgagc accaccgcgt cgacctcgtc ggagtcgcca ccggcatgca
     1861 gggcgaaccg gcggtccagt ccgggtgcca cttccttgac ggccactccg gtgcgcacca
     1921 ccacgccaag ctcggccaga taatcgccca tcggctccca caacgcctgc gggaacggct
     1981 cggcggtcac gtcgaatacc aggccctcgg ccgagcccag aaaataaata tggaacatcg
     2041 tggccagctc ggctgccgac agttcccgcg gatcggcgaa gaagctgcgg gagaacaccg
     2101 aaaacgccag gtggcgggcc gcatcgggga accgcacccg ttccaggaag gtcatcgcgt
     2161 cctggttgtc caaccgccag tagacctcgg gcactgacac gtcgatcagg ggcagcgcgg
     2221 caacgggatt gatccgagtg gccaggtcac gcaaccggaa ggtcgggctg gtcagagcga
     2281 aagccagcgc attccacggt ggagtgcggg gcagaccggc gaaggtgtcg cggtgcccat
     2341 cagcgtcgat gagcgggtag tcagacagcg gcgtaagcgc atgccccagt ggatccgctc
     2401 ggcgcagcaa cgcacgcagg ttatagtact ggcggaagaa ggcatggaag ccgcggctca
     2461 tggtgaccga ggatccgtcg gccagcgtcg tcggccagcc gcgtagccgg ccgccgagct
     2521 gctgctcgcg ctcgatcagc tgcacctgta cgccgcgctc agccagcgcc acagcggccg
     2581 cgatgccggc gatcccaccg cccaccacgg ccaccttggg aggttggccc tcggggcgca
     2641 accggccatc tcgcggcggg tgtagcacgg cacggcggtc ccggcccggt cgttgcccgc
     2701 ggtcgcctcg cggcctcact gagaccgcct ggctacgacg gtgtgcacga tgccgcgttc
     2761 ccaccctggc atcgtggcga tgccggcctc gtccaggccg tgcctgcgta gccgctgcgc
     2821 cagcgcgtcg acggtgtcaa agtgcagcac gctacgccac aggtagcggt agaggctggc
     2881 ccggccggtg accaccttcc cggcagggat gatcacagtc cagcacaccg cggtccagat
     2941 cgcccggctg cgcacccgac cgtccagcgc gtagtcgtgt atcgccagcg gtgcaccggg
     3001 tgcgagcagg tcgcgcaacc tggccagcac cgggtccggg tcgggaaggt tacgcaccag
     3061 atacgcggcg aagattccgt cgaacgggcc gtgcaccccg gccgcggcga gatcctcggc
     3121 ccgggagtgg acgaaagaca cccgctgtgg ccagtccgcc tgccgtgctc gggcgagcat
     3181 gccggccgac gcatccaccg cggtgatcgt ggcctgcggt gcggcggcaa gcagcgcggc
     3241 cgttgatgcc ccggtcccgc agcctaaatc cagcaggtgc aaccccgcgc cctgtccggg
     3301 aagctgcatc cggtgggcgg agcgggccag gttgtcgtga tagccggggc tggccgcgac
     3361 cagacggtca taattcgctg cggccgcgtc aaactcgctg ggaacgtcga tcgtcaccgg
     3421 cgcagcctac ggctgcccgt gcgcgccgat accagctatg gctggtgtcg tcactcgcaa
     3481 gcggcccagc cgcagaatgt gagcgacggc ccgccacggc tgaagtttca cgtagttggt
     3541 ctggccccag tcgtaggtct cgccgctgac ctcgtcgtgc acgagaaaac ggtcctgcca
     3601 gcgcatcccg agcgccggca ggtcaagcga gaccgtcccg ctcacggtga tgtagggctc
     3661 cagcgtcacc acgcagacaa tgacgtcgtc ggtggccggg tcggtcttgg agtacgccag
     3721 cagtgactcg tggttggtgt ggtggaaccg cag
//
LOCUS       DATADN010000166         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1074479, whole genome shotgun sequence.
ACCESSION   DATADN010000166 DATADN010000000
VERSION     DATADN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074479"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1226
                     /locus_tag="VHH53_02210"
     CDS             135..1226
                     /locus_tag="VHH53_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX2289019.1"
                     /translation="MHSDRLLLGPGPSNPYPEAVEALARPVLGHLDPEFLALVADTAQ
                     RLRTVFGTRNRLTLPVSGTGSAGMEACLASLLEPGELAIIGVNGYFGDRLCEVARRVG
                     AQVQAVHAPWGRPLDPDQLLHAQRRRPQARLVAVVHAETSTGVRNQIAPLRELQDTDT
                     LLVVDTVTSLGGIPVEVDAWGIDACCSATQKCLGAPSGIAPVTLSDRAVQRIRDRERP
                     VSSFYLDLTLLDDYYQGDPPRYHHTASSSLISAVHGGLGRLLEEGLPAVWERHARVGE
                     RLQMALPEHGFELFAEHGHRLPQLTAARLPEGVVDAKARAALREDFGIEVGGGLGPLA
                     GAGWRIGLMGHNAQDRAVTTLLAALAAWS"
     gene            complement(1275..2105)
                     /locus_tag="VHH53_02215"
     CDS             complement(1275..2105)
                     /locus_tag="VHH53_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2289020.1"
                     /translation="MSAVVTLNNGVRMPQLGFGVFQVPPEQTEAVVSTALEVGYRSID
                     TAAAYHNEEGVGKAIASSGLARDEVFVTTKLWNADHGYDRALRAFDASAQRLGLETVD
                     LYLIHWPVPAMDRYVETWKALEKLAADGRARAIGVSNFGVEHLRRLVAETGTVPAVNQ
                     VELHPYFQQTDLRAEHAEHGIATEAWSPLGQGGALLSDATITWLADRHGMTPAQVVLR
                     WHLQLGNIVIPKSANPARMRQNLDVFAFELSAEDLSAIADLETGKRMGPDPATANFGT
                     "
BASE COUNT          370 a          793 c          780 g          387 t
ORIGIN      
        1 ctgccgaccg tgactgccta gcaccacacc ggaagcgtcc accaccgcaa cggccaccgt
       61 gtccaccggc cactgcgcca cagcgcacaa gctgtccatc gcggcgacag tacgacagtg
      121 gcaagatcgg ctctgtgcat tccgatcgct tactgctcgg gccaggcccg tccaacccct
      181 accccgaggc tgtcgaggcg ctggcccgtc cggtgctcgg gcatctggac ccggagttcc
      241 tcgccctggt agccgatacc gcacaacggc tgcggaccgt cttcggtacc cgcaaccggc
      301 tcaccctccc ggtcagcggt accgggtcag ccgggatgga agcctgcctg gccagcctgc
      361 tcgagcccgg ggagctggcc atcatcggag tgaacggcta cttcggcgac cggttgtgcg
      421 aggtggcgcg ccgggtcggt gcgcaggtgc aggccgtgca cgcgccatgg ggacgacccc
      481 tggaccctga ccagctgctc cacgctcaac gccgcaggcc gcaggcccgg ctcgtcgcgg
      541 tggtgcacgc cgagacctcc accggggtcc gtaaccagat cgccccacta cgcgagctac
      601 aggacaccga caccctgctg gtcgtcgaca ccgtcacttc gctgggtggg attccggtgg
      661 aggtggacgc ctggggcatc gacgcctgct gctcggcgac tcagaagtgc ctgggcgctc
      721 cgtcagggat cgcgccggtg accctgtccg atcgggcagt gcagcggatc cgggaccggg
      781 aacggccggt cagctcgttc tatctcgacc tgaccttgct ggacgactac taccagggcg
      841 acccgccccg ctatcaccac accgcgagct caagcttgat ctctgccgtg cacgggggtc
      901 tggggcggtt gctcgaggag ggcctgccgg ccgtgtggga acgtcacgcg cgagtgggcg
      961 aacggctgca gatggcactg cccgagcacg gtttcgaact gttcgccgag cacgggcacc
     1021 ggttgccgca gctcactgcc gcccggctac ccgagggggt ggtggacgcc aaggcccgtg
     1081 ccgcgttgcg ggaggacttc ggtatcgaag tcggtggcgg tctgggaccg ttggccggcg
     1141 ccggttggcg gatcggcctg atgggccaca acgctcagga ccgcgcagtc accacgctgc
     1201 tcgctgcgct agcagcgtgg tcgtgagtgg gaaacagtgt tgtaacactg tttcccactc
     1261 acgagctcat gggctcacgt gccgaagtta gcggtggcgg gatcggggcc catccgcttg
     1321 ccggtctcga ggtccgcgat cgcggagaga tcctcggccg agagctcgaa ggcgaagacg
     1381 tcgaggtttt gccgcatccg cgcgggattc gccgacttgg ggatcacgat atttcccagc
     1441 tgcaggtgcc agcgcagcac tacctgggcc ggggtcatgc cgtgccgatc ggcgagccag
     1501 gtgatggtgg cgtcggagag cagtgcgccg ccctgaccca gtgggctcca cgcttcagtg
     1561 gcgatgccgt gctcggcgtg ctcggcgcgc aggtcggtct gctggaagta cgggtgcagc
     1621 tcgacctggt tgacggcggg caccgtgccg gtctcggcaa ccaaccgccg caggtgctcg
     1681 acaccgaagt tggacacccc gatcgcccgc gcccggccat cagctgccag cttctccagc
     1741 gccttccacg tctcgacgta ccggtccatg gcgggcaccg gccagtggat caagtaaaga
     1801 tctactgtct ccaggcccag ccgctgcgcg ctggcatcga acgcgcgtag cgctcggtca
     1861 tagccgtggt cggcgttcca cagcttcgtg gtcacgaaga cctcgtcacg ggccagcccg
     1921 gaggatgcga tcgccttccc gacgccctcc tcgttgtggt aggccgccgc ggtgtcgatg
     1981 ctgcgatagc cgacctccaa tgccgtggat accactgcct cggtctgctc cggcggcacc
     2041 tgaaagaccc caaaaccgag ctggggcatc cgcacaccgt tattcaacgt aacgaccgca
     2101 ctcataccct caacgtaccc gaatcgcgtt ctggaggcag gcagctgtta tcggtgccga
     2161 ataacagcag ctgcctccag aatgcgcaaa agggcgcgga accggggcgc acccattagg
     2221 tcgaggtggt ttgttggagc actccggcta ccggcttgag gcgcggcgag cccacgtcgg
     2281 cgtggtagtc ctgcgggtcg gtcgcgtcgg tgccgttgaa cacccgcatc
//
LOCUS       DATADN010000167         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_321143, whole genome shotgun sequence.
ACCESSION   DATADN010000167 DATADN010000000
VERSION     DATADN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321143"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..>1577
                     /gene="purB"
                     /locus_tag="VHH53_02220"
     CDS             479..>1577
                     /gene="purB"
                     /locus_tag="VHH53_02220"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2289021.1"
                     /translation="MLAVLAQRYASAELVRLWSPEHKVVLERQLWLAVLRAQADLGVP
                     VEARVLDDYQRVVHVVDLESIAARERLSRHDVKARIEEFNALAGHQEIHKGLTSRDVT
                     ENVEQLQVRCSLEHVRDRCVALLARLAGRATEYAELVMVGRSHNVAAQATTLGKRFAN
                     AADELLLAFARLEELLVRYPLRGIKGPMGTAQDTLDLLGGDASALAQLEQRVAAHLGF
                     GRLLTSVGQIYPRSLDHEVASTLVQLAAAPSSLATTIRLMAGHELVTEGFQPGQVGSS
                     AMPHKMNTRSCERVNGLAVVLRGFASMLAELSGSQWNEGDVSDSVVRRVALPDAFCAF
                     DGLVETFLTVLDEFGAYPAVIDRELERFLPFL"
BASE COUNT          246 a          515 c          543 g          273 t
ORIGIN      
        1 gttgcagcgc cgacgaaacc gggcagcggc ggctcctgcg gattgcccgc catgaagtcg
       61 gcggtcatcg gttgcccgcc ctgcaccggt ggctgcctca ccgccgccca gaacggacgc
      121 agggcacgct gcgccgcttc gacatgcctg gcgacgatca tggctacctc ggttacgcgc
      181 ctgggtgcct ccctgaggac gtcctgcaag ccgcgtgatc agtcccacgc tcaccaagcc
      241 agcgcactcg ccagacctgc gcggccctcg acgttccccc gtgatcgata ggctcacctg
      301 cacagcagag ctcaggttgt gccgatgtgc actacagcgc gacacaccgg gcaggagaac
      361 cggacacgcc agtggggccg cacgcttgcg gtgatctcaa cacccggtgc gcgaggccag
      421 cacgctccgc tagccgacga gcttcccgac cactcacgag cacggccagc acgctagcgt
      481 gctggctgtg ctggcgcagc gttatgcctc cgcggagctg gtccggctgt ggtcgccaga
      541 gcacaaggta gtgctcgagc ggcagctatg gctggcggtg ctgcgcgccc aggccgatct
      601 gggcgtgccg gtcgaggctc gagtgctcga cgactaccag cgggtggtgc acgtggtgga
      661 tctcgagtcg atcgcggcgc gggaaaggct gagccggcat gacgtcaagg cacgcatcga
      721 ggagttcaac gcgctggccg gccaccagga gatccacaag gggctgacgt cgcgggacgt
      781 cacggaaaac gtcgagcagc tgcaggtgcg ctgttccctg gagcacgtac gggatcgctg
      841 tgttgcgctg ctggcacgcc tcgcgggccg ggctaccgaa tacgccgagc tggtcatggt
      901 cgggcgcagc cacaacgtcg cggcccaggc caccacgctg ggcaagcggt tcgccaatgc
      961 agctgatgag ctgctgctgg ccttcgccag gctcgaggag ttgctggttc gctacccgct
     1021 acggggcatc aaggggccaa tgggtaccgc ccaggacacg cttgatctgc tcggtggtga
     1081 cgcgtcggcc ctggcgcagc tggaacagcg ggtggccgcg cacctgggct tcggtcggtt
     1141 gctgaccagc gtcgggcaga tctatccccg gtcgctggac cacgaggtgg ccagcaccct
     1201 ggtgcagttg gccgccgcac catcatcact tgcgacgacg atccgcttga tggccggtca
     1261 cgagctggtg accgaggggt tccaacctgg tcaggtcggg tccagtgcca tgccacacaa
     1321 gatgaacacc cggtcctgcg agcgtgtcaa tggcttggcc gtggtgctgc gcgggttcgc
     1381 ctcgatgctg gccgagctgt ccggttcgca gtggaatgag ggcgatgtgt cggattccgt
     1441 ggtgcgccgg gtcgcgttgc ccgacgcatt ctgcgccttc gacggtttgg tcgagacctt
     1501 cctcaccgtg ctcgatgagt tcggtgccta tcccgcggtg atagaccggg agctggaacg
     1561 ctttctccct tttctgg
//
LOCUS       DATADN010000168         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1221317, whole genome shotgun sequence.
ACCESSION   DATADN010000168 DATADN010000000
VERSION     DATADN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221317"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHH53_02225"
     CDS             <1..489
                     /locus_tag="VHH53_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289022.1"
                     /translation="TESLFEHTKRHCDQLEAASLRRQELAEQDAAQAIAQRDSEATDR
                     IRGSEMRSIVRVCLMLRAVDEQLTTRLGAVEREETALRELRAQAAEEATALEALRTEV
                     TAALATTHQLVTEAIGQVQQTMTEGSDDAVQVPLQRSTEGGRIYLLNAANEERRSTHP
                     SS"
     gene            575..>1672
                     /gene="rocD"
                     /locus_tag="VHH53_02230"
     CDS             575..>1672
                     /gene="rocD"
                     /locus_tag="VHH53_02230"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEX2289023.1"
                     /translation="MSGFIAADEQWSTHNYHPLPVVIATGEGAWVTDVTGRRYLDFLA
                     GYSALDFGHRHPALVAAATHQLGRLTLSSRAFHHDLFGAFCRELAELTATEMVLLMNS
                     GAEAVESAIKVARLWAHRVKGVPDGTAEIVVAGGNFHGRTTTIVSFSTDEIARAGFGP
                     FTPGFVVVPYGDLDALHGAITARTAAVLVEPVQGEAGVLVPPPGYLAAVRAACDDAGV
                     LLIADEIQSGLARTGDLLALDHERVRADLYTLGKALGGGILPVSAVVGSRAVLGVLRP
                     GEHGSTFGGNPLACAVGLAVVALLATGEFQARSRELGAHLHNRLAKLIGHGVARVTGR
                     GLWAGIHIAAGEPTGRMVTEALMARGVLCKET"
BASE COUNT          281 a          557 c          570 g          264 t
ORIGIN      
        1 accgagtcgc tgttcgaaca cacgaagcgg cactgcgacc agctcgaggc agcttcactc
       61 cggcggcagg aactcgccga acaggatgcc gcgcaggcga tcgcccagcg cgattctgag
      121 gcgaccgacc gcatccgtgg cagtgagatg cgcagtatcg ttcgggtgtg cctcatgctg
      181 cgggccgtcg acgagcagct gaccacgcgg ctcggcgcgg tcgagcggga agagactgcg
      241 ctgcgcgaac tacgagccca ggcggctgag gaggcaaccg cgctcgaagc gttgcgcaca
      301 gaggtcaccg cggcactagc caccacgcac cagctcgtaa cggaggcgat cgggcaggtc
      361 cagcagacca tgaccgaagg ttccgacgat gcggtacagg tgcccctcca gcgcagtacc
      421 gaaggcggga ggatctacct gctcaacgct gcaaacgagg aacgacggtc cacgcaccca
      481 tccagttgaa ccggtgaaac cccggcacag tgatgcggtt acgcaggcta tgaccgtcct
      541 cgacacgtca gttcctggca cctcacatca cgccatgtcc gggttcatcg ccgccgacga
      601 gcagtggagc acgcacaatt accacccgct gcccgtggtg atcgccacgg gcgaaggcgc
      661 ctgggtcact gacgtgaccg gccgccgcta cctcgatttc ctcgccggct actcggcgtt
      721 ggacttcggg caccgccatc ccgccctggt ggccgccgcg acacaccagc tgggacggct
      781 gacgctatct tcgcgtgcct ttcaccacga tctgttcggc gcgttctgcc gggaactcgc
      841 cgagttgacc gcgaccgaga tggtgctcct gatgaactcc ggagctgaag ccgtcgaatc
      901 cgcgatcaag gtggcccggc tgtgggccca ccgggtcaag ggtgtcccgg acggcaccgc
      961 ggagatcgtg gtggcaggcg gtaacttcca cggccgtacc accacgatcg tctcgttctc
     1021 caccgacgag atcgcccggg ccgggttcgg cccttttacc ccgggttttg tggtggttcc
     1081 ctacggcgat ctcgacgcac tgcacggcgc gatcacagcc cggacggccg ccgtccttgt
     1141 cgaaccggtg cagggtgagg cgggagttct ggtaccgccg ccgggttatc tcgccgcagt
     1201 ccgcgcagcc tgcgacgacg cgggcgtgct gctgatcgcg gatgagatcc aatccgggct
     1261 ggcgcgcacc ggtgacttgc tggccctgga ccacgaacgg gtgcgagccg acctttatac
     1321 cctgggcaaa gcgctgggcg ggggaattct gccggtctcg gcggtcgtcg gaagccgcgc
     1381 cgtactcggt gtgcttcggc ccggagaaca cgggtcaacc ttcggaggta acccgttggc
     1441 ctgcgcagtg ggcctcgcgg tggtcgcgct gctggccacc ggcgagttcc aggcccgctc
     1501 acgagaactg ggcgcacacc tgcacaaccg gctggccaag ctgattgggc acggggtcgc
     1561 gcgggtcacc ggccgggggc tgtgggccgg gatacacatt gctgccgggg agccgacggg
     1621 acggatggtc accgaggcgt tgatggcccg cggcgtgctg tgcaaggaga cc
//
LOCUS       DATADN010000169         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_773625, whole genome shotgun sequence.
ACCESSION   DATADN010000169 DATADN010000000
VERSION     DATADN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773625"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VHH53_02235"
     CDS             complement(<1..763)
                     /locus_tag="VHH53_02235"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX2289024.1"
                     /translation="MRQVNGGPIDYLMMDYLAEVTMSIMQKQKSRDPRAGYARDFVPL
                     MERILPDVVERGIRVTSNAGGVNPRGCAEAVLEVSRRLGLRGKVRVGLVTGDDILDRL
                     DDLIARGHPLADMETGRPLAEIRGRVLSANAYLGMAPIVEALRGRANVVVTGRVTDTC
                     LTLWPIFHEFGWSPEDWDKVAAGTVAGHIIECGAQASGGNLLKGWRAVKGLANPGFPI
                     VEASPNGTFVVTKHPGTGGVVSVASVTEQLVYEMGD"
     gene            complement(826..>1709)
                     /locus_tag="VHH53_02240"
     CDS             complement(826..>1709)
                     /locus_tag="VHH53_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289025.1"
                     /translation="RPFEVRVSSGSAGQKGLAFFHEPDGMPYRDESARPIGAAEREAV
                     YEVDGRDVVSGAYEAVVVATPVQPMEATIKVTQSPLRLQLGRERDAAVARLANLTNTP
                     ARAEVGMLLGGAERVETVVARGSAVRRIPFLAPAWAQAVVIDLTMERAQWSRFTDFGV
                     TLFDSVGRQIEKQPLNYAFGRLQAKLPERQGNQPLQLALFPAFADPNSDESWTVRAII
                     RLYADSAVALEPTAAADSVITIAPGKTATAGFSLPSNPWPLGDGFFPLAVLVARVGEH
                     SWTREGGLPLPNPAMMR"
BASE COUNT          276 a          621 c          551 g          261 t
ORIGIN      
        1 ggtcgcccat ctcgtacacc agctgctccg tcacggacgc gaccgacacc acgccgccgg
       61 tgcccggatg cttggtcacc acgaaggtgc cgttcggcga ggcctccacg atggggaatc
      121 cgggattggc caggcccttc accgcgcgcc agcccttgag gaggttgccc ccggatgcct
      181 gcgcgccaca ttcgatgatg tgccccgcca cggtgccggc agccaccttg tcccagtcct
      241 ccggcgacca gccgaactcg tggaagatgg gccagagcgt gagacaggtg tcggtcacgc
      301 gcccggtcac cacgacgttc gcgcgcccgc ggagcgcttc cacgatcggc gccatgccga
      361 ggtaggcgtt ggccgacagc acgcgaccgc ggatctccgc cagcggccgt ccggtctcca
      421 tgtcggccag cgggtggccc cgggcgatca ggtcgtccag ccgatcgagg atgtcgtcgc
      481 cggtgaccag cccgacccgc accttgcccc gcagcccgag cctgcgggag acctcgagca
      541 cggcctccgc gcagccgcgc gggttcaccc cgccggcatt cgaggtcacc cggatcccgc
      601 gctccaccac gtccggcagg atccgctcca tcagggggac gaagtcccgc gcgtagccgg
      661 cgcgggggtc gcgcgacttc tgcttctgca tgatggacat ggtgacttcg gccaggtagt
      721 ccatcatcag gtagtcgatg gggcctccgt tcacctggcg cacgggcgcc tccagccagt
      781 cgccccagaa cccctgccct gccgcaactc gtacggttcg cccgttcatc gcatcatggc
      841 cggattgggg agtgggagcc cgccttcccg ggtccagctg tgctcgccca cgcgtgccac
      901 gaggaccgcc agcgggaaaa acccgtcacc aagaggccaa gggttggacg gcaagctgaa
      961 tcccgcagtg gcggtcttcc cgggagctat ggtgatgacg gaatccgccg ccgcagtcgg
     1021 ctcgagcgcg accgcggaat cggcatagag gcggatgatg gcccggaccg tccagctctc
     1081 gtcgctgttc gggtccgcaa acgcgggaaa gagggcgagc tgcagcggct ggtttccctg
     1141 gcgctcgggc agcttggcct gcagccgtcc gaaggcatag ttgagcggtt gcttttcgat
     1201 ctgcctcccg acggaatcga agagcgtgac tccgaaatcg gtaaagcggc tccactgggc
     1261 tcgctccatc gtcagatcga ttactaccgc ctgggcccac gccggagcga gaaagggaat
     1321 gcggcgcacc gcggaaccgc gcgcgacgac ggtctcgacc cgctcggccc cgccgagcag
     1381 catcccaacc tccgcgcggg caggagtgtt cgtcaggttg gcaagccgcg caaccgcggc
     1441 gtcccgctcc cgtcccaact gcagccggag cggagactgg gtcaccttga tcgtggcctc
     1501 catcggctgc accggagtcg ccacaaccac ggcctcgtag gccccggaga caacgtctcg
     1561 tccatcgacc tcatacaccg cctcgcgttc ggccgctcca atgggccgcg cactctcgtc
     1621 ccgatacggc attccgtccg gctcgtgaaa gaacgcgagt cccttctgtc ctgcgctccc
     1681 gctggaaacg cggacctcaa aaggacgcg
//
LOCUS       DATADN010000170         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1075513, whole genome shotgun sequence.
ACCESSION   DATADN010000170 DATADN010000000
VERSION     DATADN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075513"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2114
                     /gene="carB"
                     /locus_tag="VHH53_02245"
     CDS             <1..>2114
                     /gene="carB"
                     /locus_tag="VHH53_02245"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2289026.1"
                     /translation="PDPPDATLDSVLAALEVPHDGRLYTAERALRLGATVTQVAQASG
                     IDPWFVDQIAGLVELRAELVVAPVLDQELLRRAKRAGISDRQLAAVRSDLTEPEVAAL
                     RHREGVRPVYKTVDTCAAEFAARTPYHYSAYETDPDAETEVTQQRERPKVIILGSGPN
                     RIGQGIEFDYSCVHAAMALRAVGYETVMVNCNPETVSTDYDTADRLYFEPLTFEDVME
                     VIEAERTSGTVAGVIVQLGGQTPLGLAQRLADAGVPVVGTSPEAIHLAEDRGSFGAVL
                     ERAGLPAPAYGTATSFAEARKIAEEIGYPVLVRPSYVLGGRGMEIVYDEETLAGYIAR
                     ATEITPEHPVLVDRFLDDAIEIDVDALCDGNEVYLGGIMEHIEEAGVHSGDSSCALPP
                     IALGESDLVTVRSSTEAIARGIGVRGLLNVQYALKGDVLYVLEANPRASRTAPFVSKA
                     TAVPLAKAAARIMLGASIAQLRAEGMLLAHGDGGDLPADAPVAVKEAVLPFHRFRTPE
                     GQGVDSLLGPEMKSTGEVMGIDAAFGQAFAKSQAAAYGSLPTSGRVFVSVANRDKRAM
                     VFPAKRLADLGFEVLATRGTANTLRRNGIPCTVVRKHFEGPDNIVELINAGEIDMIIN
                     TPYGNPGPRVDGYEIRTAAVSRDIPCITTVQGAAAAVQGIEALIRNDITVRPLQALHT
                     ALRAAPTTPTTPHSAGSAGLSD"
BASE COUNT          336 a          750 c          698 g          330 t
ORIGIN      
        1 gcccgatccg ccggacgcca ccctggattc ggtactagca gcgctggagg tgccccacga
       61 cggccggctc tacaccgccg agcgggcgtt gcggctgggc gcgacggtca cccaggtggc
      121 gcaggcgtcg ggtatcgacc cgtggttcgt cgaccagatc gccggcctgg tcgagctgcg
      181 cgccgaactc gtcgtggcgc cggtactgga ccaggagttg ctgcgccgcg ccaagcgcgc
      241 cggtatctcc gaccggcagc ttgccgccgt gcgcagtgac ctcactgaac ccgaggtagc
      301 cgcgttgcgg caccgcgagg gtgtgcggcc ggtctacaag accgtggaca cctgcgctgc
      361 cgagttcgcc gcccgcacgc cataccacta ctccgcctac gagaccgatc ccgatgccga
      421 gaccgaggtc actcagcagc gggagcgacc caaggtgatc attctcggct ccgggccgaa
      481 ccggatcggg cagggcatcg agttcgacta ctcctgcgtg cacgctgcga tggcgctgcg
      541 cgccgtcggc tacgagaccg tgatggtcaa ctgcaacccg gagaccgtgt ccaccgacta
      601 tgacaccgcc gaccggctct acttcgagcc gctgactttt gaagatgtca tggaggtgat
      661 cgaggccgag cggacctcgg gcaccgtggc gggtgtgatc gtgcagctgg gtgggcagac
      721 cccgctgggg ttggcgcagc ggctggccga tgcgggcgtc ccggttgtcg gcacctctcc
      781 ggaagcgatt cacctcgccg aggaccgcgg ttccttcggc gcggtgctgg agcgggccgg
      841 gctacccgca ccggcgtatg gcaccgccac ctcgttcgcg gaggctcgca agatcgccga
      901 ggagatcggt tacccagtcc tggtccgtcc ctcctacgtg ctcggtgggc gcgggatgga
      961 gatcgtctac gacgaggaga ccctggccgg ttacatcgcc cgtgccaccg agatcacccc
     1021 cgagcacccg gtgctggtgg accgcttcct tgacgacgcc atcgagatcg acgtcgacgc
     1081 gctgtgtgac ggcaacgagg tgtacctcgg cgggatcatg gaacacatcg aggaggccgg
     1141 agttcactcc ggggactcct cctgcgcgtt gccgccgatc gcgctcggcg agtccgacct
     1201 ggtcacggtg cggtcctcga ctgaggcgat cgcccgcggt atcggcgtcc gcggcctgct
     1261 caacgtgcag tacgccctca agggcgacgt gctctacgtg ctggaggcca acccacgagc
     1321 cagccgcacg gcgccgttcg tctccaaggc caccgcggtg ccactggcca aagccgctgc
     1381 ccggatcatg ctcggtgcca gcatcgcgca gctgcgagct gaaggcatgc tgctcgcgca
     1441 cggggacggg ggcgacctgc cggccgacgc gccggtggcg gtcaaggagg ccgtgctgcc
     1501 cttccaccgg ttccgcaccc cggaaggtca aggggtggac tccctgctgg gaccggagat
     1561 gaagtccacc ggcgaggtga tgggcatcga cgccgcgttc ggccaagcct tcgcgaagtc
     1621 ccaagccgcg gcgtacggct cgttaccgac ctccgggcga gtgttcgtgt cggtggccaa
     1681 ccgggacaag cgggccatgg tgtttcccgc caaacggctg gccgatttgg gcttcgaggt
     1741 cctcgccacc cgcggaaccg ccaacacgct gcgccgtaac gggattccct gcaccgtcgt
     1801 gcgcaagcac ttcgaaggtc ccgacaacat cgtcgagttg atcaacgccg gtgagatcga
     1861 catgatcatc aatacgccct acggcaatcc gggtccccgg gtggacggtt acgagatccg
     1921 caccgccgcg gtatcccggg acatcccctg catcaccacc gtgcagggcg ctgctgcagc
     1981 cgtccaaggc atcgaagccc tcatccgcaa cgacatcacc gtccgtcccc tccaagccct
     2041 ccacaccgcc ctccgcgccg cccccaccac ccccaccacg ccgcattcag cgggctcagc
     2101 ggggttatcc gatg
//
LOCUS       DATADN010000171         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_321929, whole genome shotgun sequence.
ACCESSION   DATADN010000171 DATADN010000000
VERSION     DATADN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321929"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VHH53_02250"
     CDS             complement(<1..199)
                     /locus_tag="VHH53_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEX2289027.1"
                     /translation="MTAVRQMPPEVAPPRLGLLLRVLPSGLYAGRSRAVILRSSIASR
                     SVWLAIVSGFFEPLFFLVAMGQ"
     gene            complement(196..1023)
                     /locus_tag="VHH53_02255"
     CDS             complement(196..1023)
                     /locus_tag="VHH53_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289028.1"
                     /translation="MTTVEAVRCTGRVLPGWQAALLVVEARWIWYRRNWRATAISSLL
                     QPLLFLVAFGIGFGSLISASDRAVQFTGGVAYLTYLAPALLVIGAIQTAAFESTYPIL
                     SAFKWQRTYWAVTATPVTSGQLAGGQLTWIALRLTLSSAAYLLVVVLFGAMHGLGVLA
                     SLVFAVLSGMAFSAPLVAFSAAQEGEGTMFAAVFRFVVVPMTLFSGTFFPISQLPELI
                     QPLAWISPLWHGTELARGVALGTMTLWSAVGHAAYLTALLLVGVLLSRWRFRVRLTR"
     gene            complement(1023..>1856)
                     /locus_tag="VHH53_02260"
     CDS             complement(1023..>1856)
                     /locus_tag="VHH53_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2289029.1"
                     /translation="ETFGFLGPNGAGKSSAMRMIACVSPRSAGELQVLGMDPDADGPR
                     IRARLGVVPQQDNLDTELSVRENLHVYGRYFGMSRAAVRDKAAELLDFAQLTDRAGDP
                     VDLLSGGMKRRLTIARSLVNDPELMLLDEPTTGLDPQARHLLWDRLFQLKQQGVTLII
                     TTHYMDEAEQLCDRLVVMNGGLIVAEGSPGELIARYSTREVLELRFRPGDQHSWFDAV
                     DGLADRIEKLPDRLLLYVLDGEAALSAAHQRGVRPMSSLVRRSTLEDVFLRLTGRSLV
                     D"
BASE COUNT          383 a          640 c          580 g          253 t
ORIGIN      
        1 cctgtcccat cgccaccaga aagaacagcg gctcgaagaa tccggagacg attgccaacc
       61 agacactccg cgacgcaatc gaggagcgca ggatgaccgc tcgggaccgc ccggcgtaca
      121 accccgatgg cagcactcgc agcagcaatc ccaaccgcgg cggcgcaacc tccggaggca
      181 tctgtcggac cgcggtcatc gggtcaacct gacccggaac cgccaccgcg acagcaacac
      241 tcctaccagg agcaacgccg tcaaataagc ggcgtgcccc accgcagacc aaagcgtcat
      301 ggtgcccagc gcgacaccgc gggcgagttc ggtgccgtgc catagcgggc tgatccaggc
      361 cagcggctgg atcaactcgg gcagctgcga aatcgggaag aacgtgccgg agaacagcgt
      421 catcggcacc accacgaacc ggaacacggc agcgaacatg gtgccctcgc cctcctgcgc
      481 ggcggagaac gccaccagcg gcgcgctgaa cgccataccg gacagcactg cgaagaccag
      541 cgaagccagc accccgagcc cgtgcatcgc gccgaatagc acaaccacca ggaggtatgc
      601 ggcgctggat aaggtcagcc gcaaagcgat ccaggtgagc tgcccgcccg ccagctgtcc
      661 ggatgtgacc ggggtagccg tgacagccca gtaggtgcgt tgccatttga acgccgacag
      721 gatcgggtag gtcgactcga aggcggctgt ttggatcgcg ccgatcacca gcaacgcagg
      781 tgcgagatag gtgaggtagg ccaccccgcc ggtgaactgc accgcccggt cgcttgcact
      841 aatcaagctg ccgaagccga taccgaaggc gactaggaac aacagcggct gcagcaggct
      901 ggagatggct gtggcccgcc agttgcgccg gtaccaaatc caccgcgcct cgacgacgag
      961 cagcgcggcc tgccacccgg gcagcacccg accggtacaa cgaacagcct ccaccgtggt
     1021 catcagtcca ccagcgatcg cccggtgagc cggaggaaca cgtcctccag ggtggatcgt
     1081 cggaccaggc ttgacatcgg tcgcacgcca cgctggtgcg cggcggacag cgctgcctcg
     1141 ccgtcgagga cgtaaagcag caaccggtcg ggtagcttct cgattcgatc ggccaagccg
     1201 tccaccgcat cgaaccagct gtgttgatcg ccaggacgga atcgcaactc gagtacctcg
     1261 cgagtggagt accgagcgat cagctcaccc ggtgagcctt ccgcaacgat cagcccgccg
     1321 ttcatcacta cgagccggtc gcacagctgt tcggcctcgt ccatgtagtg cgtcgtgatg
     1381 atcagcgtga cgccttgctg cttgagctgg aacaaccggt cccacagcaa gtgccgggcc
     1441 tgcgggtcca atccagtggt cggctcatcg agcagcatca gctccggatc gttgaccagg
     1501 gaccgggcga tggtcagccg ccgcttcata cccccggaca gcaggtcgac ggggtcgcca
     1561 gcccgatcgg tgagttgcgc gaaatccagg agctcggcgg ccttgtcccg cactgcggcc
     1621 cgggacatgc cgaagtagcg cccatagaca tggaggttct cccgcaccga cagctcggtg
     1681 tccaagttgt cctgttgcgg caccaccccg agccgcgcgc gaatccgtgg cccgtcagcg
     1741 tccggatcca tccccagcac ctgcagctcg ccggcgctgc gtggggacac gcaagcgatc
     1801 atgcgcatgg cggaggactt gcccgcaccg ttggggccca ggaaaccgaa cgtctc
//
LOCUS       DATADN010000172         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_472865, whole genome shotgun sequence.
ACCESSION   DATADN010000172 DATADN010000000
VERSION     DATADN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472865"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VHH53_02265"
     CDS             <1..761
                     /locus_tag="VHH53_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX2289030.1"
                     /translation="VIIFTGSLSTGDIVDAQQRFWYALVLLPSFVIYLVSMVGETNRA
                     PFDLPEAESELVGGFHTEYSSMKFALFFLAEYVNMVIVSAFATTLFLGGYMAPWPISL
                     INDNYFNSGWWPLLWFFGKTLAVLFVYIWLRATLPRMRYDQFMRIGWKVLVPLNLLWI
                     VAVIAVNVIVDEGLTTSQWLLGIGIVLVIIVGLSLLLPERQVPDEDQVPVTGSGYPVP
                     PLDLEIPGKPKRKVRSPRRERARVGAGDEQKETD"
     gene            763..1311
                     /gene="nuoI"
                     /locus_tag="VHH53_02270"
     CDS             763..1311
                     /gene="nuoI"
                     /locus_tag="VHH53_02270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX2289031.1"
                     /translation="MGFFDAVKGFGVTFSTMFKKVVTEEYPEAGAPAAPRYHGRHQLN
                     RHPDGLEKCVGCELCAWACQADAIFVEGGDNTEEARYSPGERYGKDYQINYLRCIGCG
                     LCIEACPTRSLTMLNEYELADDNRQDLIWTKEQLLAPLLPGMEQPPHPMRLGESEQDY
                     YVNGKQLARKWGYPDPVGEETK"
     gene            1323..2186
                     /locus_tag="VHH53_02275"
     CDS             1323..2186
                     /locus_tag="VHH53_02275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX2289032.1"
                     /translation="MVLAQAAAEVSTGEAVAFWILGPMALAGAVGMIFARNAVHSALF
                     LVLTMFSLGLLYMVQQAPFLGFVQIIVYTGAIMMLFLFVLMLVGRDSSDSVVEVLRGQ
                     RLWAAVLGIGLGGLVIGALARAIVSVSPVGLTGPDTNAGRGNVHNIGRLLYTDYLFPF
                     ELTSALLITAAVGAMVLGYVDRHVRGRRTQKELVVARFRGEHDRPSPLPGPGVFATSN
                     SVATPALLPDGSVAPESLSGLIESTAAEQFIEERKLVAGTAPTPGARALVGGQPGEPS
                     NEPASPEEERP"
     gene            2183..>2368
                     /gene="nuoK"
                     /locus_tag="VHH53_02280"
     CDS             2183..>2368
                     /gene="nuoK"
                     /locus_tag="VHH53_02280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX2289033.1"
                     /translation="MTPTYYLLLSGLLFTIGAVGVLVRRNAIVVFMCIELMFNAVNLT
                     LVTFSRINGTLDGQVMAF"
BASE COUNT          360 a          774 c          815 g          419 t
ORIGIN      
        1 cggtgatcat cttcaccggt tcactgtcca caggggacat cgtggacgcc cagcagcgct
       61 tctggtacgc gctggtgctg ctccccagct tcgtgatcta cctggtgtcc atggtgggtg
      121 agaccaaccg ggccccgttc gacctgccgg aggcggagtc cgagctggtc ggtggcttcc
      181 acaccgagta cagctcgatg aagttcgcgc tgttcttcct tgccgagtac gtgaacatgg
      241 tgatcgtctc ggcgttcgcc accacgctgt tcctcggcgg gtacatggcc ccgtggccga
      301 tatcgctgat caacgacaac tacttcaaca gcggttggtg gccgctgctg tggttcttcg
      361 gcaagacgct ggcggtgctg ttcgtctaca tctggctgcg ggccacgctg ccccggatgc
      421 gctacgacca gttcatgcgg atcggctgga aggtgctggt tccgctcaac ctcttgtgga
      481 tcgtcgcggt gatcgcggtc aacgtcatcg tcgacgaggg cctgaccacc agccagtggc
      541 tgctcggcat cggcatcgtc ctggtcatca tcgtgggact gtcgctgctg ctgcccgagc
      601 ggcaggtgcc cgacgaggac caggttccgg tcaccggcag cggctacccg gtgccgccac
      661 tggatctgga gatacccggc aagcccaagc gcaaggtgcg gtcaccgagg cgggaacggg
      721 ctcgagtcgg agccggcgac gagcagaagg agaccgactg atatgggatt cttcgatgcg
      781 gtcaagggtt tcggtgtcac cttctcgacg atgttcaaga aggtcgtcac cgaggagtac
      841 ccggaggccg gcgcaccggc cgcaccgcgt taccacggtc gccatcagct caaccggcac
      901 ccggacgggc tggagaagtg tgtcggctgc gagctgtgcg cctgggcctg ccaggccgac
      961 gcgatcttcg tcgagggcgg ggacaacacc gaggaagcgc ggtactcgcc cggtgagcgc
     1021 tacggcaagg actaccagat caactacctg cggtgcatcg ggtgtggcct gtgcatcgag
     1081 gcctgcccga cccggtcgct gacgatgctc aacgagtacg agctcgccga cgacaaccgg
     1141 caggacctga tctggaccaa ggagcagctg ctcgccccgc tgctgcccgg catggaacag
     1201 ccgccacacc cgatgcgcct cggcgagtcc gaacaggact actacgtcaa cggcaagcaa
     1261 ctcgcccgca agtggggcta ccccgacccg gttggggagg agaccaagtg accacacctg
     1321 ggctggtact ggcccaggcc gcggcggaag tgtccaccgg ggaggccgtc gcgttctgga
     1381 tcctcgggcc gatggcgctg gccggagcgg tcggcatgat cttcgcccgc aacgcggtgc
     1441 actccgcgct gttcctggta ctgaccatgt tcagcctggg cctgctgtac atggtgcagc
     1501 aggccccgtt cctcggcttc gtgcagatca tcgtctacac cggcgcgatc atgatgctgt
     1561 tcctgttcgt gctgatgctg gtgggccgtg actcgtccga ctcggtcgtc gaagtgctgc
     1621 gcgggcagcg gttgtgggcg gcggtgctgg gtatcgggct gggcggcctg gtgatcggcg
     1681 cgctggcccg ggccatcgtg tcggtgtcgc cggtgggcct caccgggccg gacaccaacg
     1741 ccggcagggg caacgtccac aacatcggcc ggctgctgta caccgactac ctgttcccgt
     1801 tcgagctcac ctcggcgctg ctgatcaccg cggcggtggg ggcgatggtc ctcggttacg
     1861 tcgaccggca cgtgcgcggc cggcgaacgc agaaggagct ggtggtggcc cggttccgcg
     1921 gcgagcacga ccggccgtcg ccgctgcccg ggcccggggt gttcgcgacg tccaactcgg
     1981 tggccacgcc ggcgctgctg ccggacggct cggtggcccc ggagtcgctg tccgggctca
     2041 tcgagtcgac cgcggccgag cagttcatcg aggagcgcaa gctggtcgcc ggcaccgccc
     2101 cgacacccgg cgcgcgcgcc ctggtcggcg gtcaacccgg cgagccgtcg aacgagccgg
     2161 catcaccgga ggaggagcgg ccgtgacgcc gacgtactac ctgctgctgt ccggactgct
     2221 gttcaccatc ggcgccgtcg gagttctggt gcggcgcaac gcgatcgtgg tgttcatgtg
     2281 catcgagctg atgttcaacg cggtcaacct gacgctggtc accttctccc ggatcaacgg
     2341 cacgctcgac ggccaggtga tggccttc
//
LOCUS       DATADN010000173         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_774396, whole genome shotgun sequence.
ACCESSION   DATADN010000173 DATADN010000000
VERSION     DATADN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774396"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VHH53_02285"
     CDS             <1..289
                     /locus_tag="VHH53_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289034.1"
                     /translation="AGHEDDKQLPVARSTDVMWVDRKNAEESEVIKALTEAFVISLGP
                     DAPELVAHRARWLVRVMTSLMLFPGHNEDDERSMLEEFVVPIVLPSRQATQ"
     gene            complement(291..968)
                     /locus_tag="VHH53_02290"
     CDS             complement(291..968)
                     /locus_tag="VHH53_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEX2289035.1"
                     /translation="MLKLLRAGDYRLTVTGKREISPHYLRLSFDAGGMLADRPLHPTM
                     WIRMWFADGDKLHQRGYTLVDPNPAADTVDIEFALHDGTASNWAQNAQPGDTIEVTVL
                     GSNFTLPEPSPAGYVIVGDTASLPAINSLLTAIGDAPARVFLEAGHDEDKHLPVARST
                     DIVWVDRKNAGEALVEAVRSSAFDASEHFGWVASDNRTTRAVAKVFREEYKIPKKSIK
                     AQAYWVA"
     gene            complement(1069..>1787)
                     /locus_tag="VHH53_02295"
     CDS             complement(1069..>1787)
                     /locus_tag="VHH53_02295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2289036.1"
                     /translation="IYTDHYLYSFLHQGLTQMWSLAVEVAYYVVLPLLAWLLLVALCR
                     RRWRPGPLMGGLVALALITPAWLILVHTTDFLPDGARLWLPAYLAWFVGGMALAVLQP
                     LGVRAYALACIPLAVACYFIVSTPIGGEPTTSPNELRAALVKALFYTVIATLAVAPLA
                     LGDRGLYARLLASRPMVFLGEISYEIFLIHLITMELAMVEILHYPIYTGSIVMLFLVT
                     FVITVPLAWLLHRFTRVRSA"
BASE COUNT          319 a          585 c          605 g          278 t
ORIGIN      
        1 ggccggccac gaggacgaca aacagctacc ggtggcccgc agcaccgatg tgatgtgggt
       61 cgaccgcaag aacgccgagg agtccgaggt gatcaaggcg ttgaccgagg cgttcgtgat
      121 ctcgctgggt cccgacgccc ctgaactggt cgcgcaccgg gcccggtggc tggtgcgggt
      181 gatgacgtcg ctgatgctgt tccccggtca caacgaggac gacgagcggt cgatgctcga
      241 ggagttcgtc gtgccgatcg tgctgccgag ccgccaagcc acgcagtagc tcaggcgacc
      301 cagtacgcct gggccttgat cgacttcttc gggatcttgt actcctcgcg gaacaccttg
      361 gccaccgcgc gggtggtgcg gttgtcggag gcgacccagc cgaagtgctc ggaggcgtcg
      421 aacgccgacg accgcacggc ctcgaccaga gcctcgccgg cgttcttgcg gtccacccag
      481 acgatgtcgg tgctgcgcgc caccggcagg tgcttgtcct cgtcgtggcc ggcttccagg
      541 aagacccgcg ccggcgcgtc gccgatggcg gtcagcagcg agttgatcgc gggcagcgac
      601 gcggtgtcgc cgacgatcac gtagccggcg ggcgagggct cgggcagcgt gaagttgctg
      661 ccgagcacgg tcacctcgat ggtgtcgccg ggttgggcgt tctgcgccca gttcgacgcg
      721 gtgccgtcgt gcaacgcgaa ctcgatgtcg acggtgtcgg cggccgggtt cgggtccacc
      781 agcgtgtaac cgcgttgatg cagtttgtcg ccgtcggcga accacatgcg tatccacatc
      841 gtcgggtgaa gcggccggtc cgcgagcatc ccgcccgcat cgaaactcag ccgcaggtaa
      901 tgcgggctga tctcgcgctt gccggtcacg gtcagccggt agtcgcccgc gcgcagcagc
      961 ttgagcaccg caccctggaa accgcgcgtt ggtttcttgt ccgacataag tagccctccg
     1021 tgtcgctgtc ccggttaggc taccctaaga ttccgttggt gcgaggtgtc atgccgatcg
     1081 cacccgggtg aaccggtgca gcagccacgc cagcggcacg gttatcacga acgtgacgag
     1141 gaacagcatg acgatcgaac cggtgtagat cgggtagtgc aggatctcca ccatcgccaa
     1201 ctccatcgtg atcaggtgga tcaggaagat ctcgtaggag atctcgccga gaaagaccat
     1261 gggcctgctg gccagcagcc tggcgtagag acctcgatcg cccagcgcca gcggcgccac
     1321 cgccagcgtg gcgatcaccg tgtagaacaa cgccttcacc aacgccgcgc gcaactcgtt
     1381 gggcgaggtg gtgggttccc ccccgatggg ggtggacacg atgaaatagc acgcgaccgc
     1441 caacgggatg cacgccagcg cgtaggcccg cacccccagc ggctgcaaga cggccagcgc
     1501 catcccgccg acgaaccagg cgagatacgc cggcagccac agccgcgcac cgtcgggcag
     1561 aaagtcggtg gtgtgcacca ggatcagcca cgccggggtg atcaacgcga gtgcgaccag
     1621 tccgcccatc agcgggcccg gccgccaccg tcgtcggcac agcgccacca gcagcagcca
     1681 ggccagcagc ggcagcacga cgtagtatgc gacctcgacg gccaggctcc acatctgcgt
     1741 caggccctgg tgcaggaacg aatagaggta gtgatcggtg taaatct
//
LOCUS       DATADN010000174         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_622337, whole genome shotgun sequence.
ACCESSION   DATADN010000174 DATADN010000000
VERSION     DATADN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622337"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1187
                     /locus_tag="VHH53_02300"
     CDS             252..1187
                     /locus_tag="VHH53_02300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2289037.1"
                     /translation="MLIRPLRAADLPAADRVRRLAFGTFLGLADPLSFRGDASPVRTR
                     YLADPSASFAAEVDGELVGTNFAVNWGSVGFFGPLSVHPNFWGRGIAQRLVETALEAF
                     ARSETRHIGLFTFSHSSQHILLYQKFGFWPRFLTPIMAKPVGRVSVDVSWSVYSALAE
                     AGQKDCLADCRQLTDAWYGDLDLSLEINATARHHFGETVLLRDGKGLIGFAVCHCGAE
                     TEAGSGQCYVKFGAVRSGPDAEPSFTRLLAACEAFALERGMARLVAGVNTARREAYRA
                     MLACGFRTDVQGLAMDRPDEAGYNRPGVYIIDDWR"
     gene            1213..>1608
                     /locus_tag="VHH53_02305"
     CDS             1213..>1608
                     /locus_tag="VHH53_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2289038.1"
                     /translation="MPAAEDVAVVRRLYAALAAGDLAAVGECFDEDAVWHLPGANALS
                     GTHRGWSAIQDNLLARQRPLSGGTFRAQLLDLAVGDRYIVAIVHATAERPGHRLDQTV
                     CQLMRVRKGKIVEVRGHYADKAALNAFWGP"
BASE COUNT          264 a          497 c          547 g          300 t
ORIGIN      
        1 agtgtctcag ctgattgtac ggcaatttgc cgtacaagtc cactactcgt cagcacttca
       61 cgatcgtccg cgacatcgac cattcagcaa ctgacaatcg tccaagatga gatcgagacc
      121 acacgattcc gcttgacggc gtaggatcct gcagccataa agagggaccc ggaccttggt
      181 gtcagtccta gttgttgccg ggtgggcgtc ctatggacac ggaagcagac agaccaaggg
      241 ccggaactga cgtgttgatc cgcccgttgc gagcggcgga tctgccggct gccgataggg
      301 ttcgtcgcct cgccttcggt accttcctcg ggctggcgga tccactgagc tttcgcggcg
      361 acgccagtcc ggtgcgaacc cgctacctgg ccgatccgtc cgcgagcttt gctgccgagg
      421 tggacggcga actggtcggg accaacttcg cggtcaactg gggcagcgtc gggttcttcg
      481 ggccgctctc ggtccacccg aacttctggg gacggggcat tgcccagcgc ctggtcgaga
      541 ccgccctcga ggcattcgcg cgctcggaga cccggcacat tggcctgttc accttctccc
      601 atagttcaca gcacatcctg ctctatcaga agttcggctt ttggccccga ttcctcaccc
      661 cgatcatggc caaacccgtc ggtcgggtca gcgtcgacgt ctcgtggtcc gtgtattcgg
      721 cgttggcaga ggccgggcag aaagactgtc ttgccgactg ccgccagttg accgatgcct
      781 ggtatggcga cctggacctc agcctggaga tcaacgcaac ggcgcggcac cacttcgggg
      841 agacggtgct gctccgggac ggcaagggac tgattggctt cgccgtctgc cactgcggcg
      901 cggagactga ggcgggcagc ggtcagtgct acgtcaaatt tggggcggtg cgctccgggc
      961 ccgatgcgga gccctcattc acccggttgc tcgcggcctg cgaagcgttc gcactggaac
     1021 gagggatggc acgattggtg gccggggtca acacggcgcg ccgtgaggcg taccgcgcga
     1081 tgctggcgtg tgggtttcgc actgacgtgc agggactagc gatggaccgg ccggacgagg
     1141 cgggttacaa ccggcccggc gtctacatca tcgacgactg gcgatagcca tggcgtgtgg
     1201 ccggggggcg tgatgccggc ggcggaagat gtggccgtgg tgcgccgcct gtatgcggcg
     1261 ctggcggctg gggacctggc cgcggtcggg gagtgcttcg acgaggacgc cgtctggcac
     1321 ctgcctgggg ctaatgccct ttccggcacg catcgcggct ggtctgccat tcaggacaac
     1381 ctcctggcca ggcagaggcc gctcagcggc ggcacattcc gcgcgcaact gctcgatctg
     1441 gccgtgggag ataggtatat cgtcgccatc gtgcacgcta ccgctgagcg tcctggtcac
     1501 cggctggacc agacggtctg ccagctcatg cgagtccgga agggaaagat cgtcgaggtg
     1561 cgcggtcact atgcggacaa agcggcgctg aacgcattct ggggaccg
//
LOCUS       DATADN010000175         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1375785, whole genome shotgun sequence.
ACCESSION   DATADN010000175 DATADN010000000
VERSION     DATADN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375785"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VHH53_02310"
     CDS             complement(<1..835)
                     /locus_tag="VHH53_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2289039.1"
                     /translation="MTDTNIQRVSDTQGRPAWLVSGHERVRDLLADPRLSRSHPNPGH
                     PVRLTQPGLFGGAIPDPEGELADLHRMRKLLTPSLSARRMEGLRPRVEAIVDELLDEL
                     ARSGPPADFHAIVAVPLPALVVCELLGVPTEDREDFRRWQSEGKQVSKPAVARGGLQS
                     LWRYLGTLIERKRWVPGDDVISDLLHAAELDPELTDDGIAHLAAGLLFAGHAPVVAII
                     DRGMLLLLTHPDQRETLQANPALAPRAVEEIFRFSSPIERMRTARRGGLTRYASVDVD
                     VD"
     gene            complement(967..>1647)
                     /locus_tag="VHH53_02315"
     CDS             complement(967..>1647)
                     /locus_tag="VHH53_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289040.1"
                     /translation="LIAAAGTALLTLWLHQLVLGALSTGTRRIVLRMLMPVAAVAGLV
                     LLILAIPTFPSFPEARGLSLSIGVLLLFYAARLLRSLSAEQRPKQVSRRAPTTAIVAE
                     WGAVFILVSVGLFWAVGSYAIGAGIQRAYQLETSLLPSSSDVVVYSEKRLNLQAAGVR
                     EMTCQYPDAAYGFRYDGLKLVLQSGNQYLFLPAGWTRADGVAMLIPRSDALRLEFRAP
                     GQPGLAGC"
BASE COUNT          285 a          531 c          569 g          262 t
ORIGIN      
        1 cgtctacgtc gacgtcgacg gacgcgtagc gggtcagccc cccccggcgg gcggtgcgca
       61 tccgttcgat cgggctggag aagcggaaga tctcctcgac ggcccgggga gcgagagccg
      121 ggttggcctg cagcgtctcc cgctggtccg ggtgggtcag cagcagcagc atgccccggt
      181 cgatgatggc gacgacgggt gcgtggccgg cgaagaggag tccggcggcg agatgggcga
      241 tcccgtcgtc ggtcagctcc gggtccagct cggcagcgtg cagcaggtcc gagatgacgt
      301 cgtcgcctgg cacccagcgc ttgcgctcga tcagggtgcc caggtaccgc cacagtgact
      361 gcaggccacc tcgggcgacg gcaggcttgc tgacctgttt gccctcactc tgccaccggc
      421 ggaagtcctc gcggtcttcg gtcggcaccc cgagcagctc gcagaccacc agcgccggta
      481 gcgggacggc gacgatagcg tggaagtcag ccggcggccc gctgcgggcg agctcgtcga
      541 gcaactcgtc gacgatcgcc tcgacgcggg gacgcagacc ttccatgcga cgggccgaca
      601 acgagggtgt cagtagcttg cgcatccggt gcaggtcagc gagctcgcct tccggatcgg
      661 ggatggcccc gccgaacagg ccgggctggg tgaggcgcac cgggtggccc gggttgggat
      721 gcgaacggga cagccgtggg tcggccagca gatcgcgcac ccgctcgtgg cctgacacga
      781 gccaggcagg acggccctgg gtgtcactta cccgttggat gttggtgtct gtcattcgcc
      841 gcccctcggt gccgcgctcg gccggatgcg acggagcgca cgattcgatc gcagaataac
      901 tggatcttcg cagcgaagcg gggagtggcc tgcgtccgaa cgggcgtcag agccggtggt
      961 tcggcgttag caaccagcca gtcctggttg gcccggtgcc ctgaactcca gtcgcagcgc
     1021 atcgctgcga ggaatcaaca tggctacgcc gtcggcgcgt gtccacccgg cgggaaggaa
     1081 gagatactgg ttgccagact gcagcacgag cttgagcccg tcgtagcgga atccgtatgc
     1141 cgcgtcgggg tattggcatg tcatttctcg gacgcccgct gcttgcaggt tcagtctctt
     1201 ctcgctgtac accacgacat ctgaactgct aggtagtagc gaggtctcaa gttgataagc
     1261 acgctggatc ccggcgccga tcgcgtagct cccgactgcc cagaacaatc cgacgctcac
     1321 cagtatgaac acagcgcccc actcggctac gatcgccgtt gtcggcgcac gccttgacac
     1381 ctgctttggc cgttgctccg cgctgagcga ccgcaacagc cgggcggcgt agaacagcag
     1441 caggacgcca atcgacaggc tgagcccacg ggcttccgga aaactcggga aagtgggtat
     1501 agccagaatc agcaggacca gcccggcaac ggccgcgact ggcatcagca tgcgcagcac
     1561 tatccgcctg gttccggtgg atagtgcacc gagcaccagc tggtggagcc atagcgttaa
     1621 caaagcagtc cccgcggcgg cgatcag
//
LOCUS       DATADN010000176         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_171484, whole genome shotgun sequence.
ACCESSION   DATADN010000176 DATADN010000000
VERSION     DATADN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171484"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1015
                     /locus_tag="VHH53_02320"
     CDS             119..1015
                     /locus_tag="VHH53_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2289041.1"
                     /translation="MLGSFFEAEDAVQETMVRAWRGIDGFEGRSALRSWLYRIATNVC
                     LDMLRGRERRARPMDLGPSSTAEGSLGPVLPEHAWVQPVPDARVLPGDSDPAELAVSK
                     ESVRLAFVTALQHLPAKQRAVLILREVLRWHATEVAELLETSVASVNSALQRARSTLA
                     ARDASEVSAFSPAVNADQRALLARYVDAFERYDITSLVSLLHEDAVMSMPPYDFWLRG
                     PVEMGRWFLGQGCGCRGSRLVPTAANGCAAFGSYRPDPDGGHRPFALQVIEISGDRIV
                     GHHNFLDTALFAAFGLPDRLES"
     gene            complement(1010..1387)
                     /locus_tag="VHH53_02325"
     CDS             complement(1010..1387)
                     /locus_tag="VHH53_02325"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX2289042.1"
                     /translation="MLELLDLLGLGEVVSDIAGLTERTRNMGITFPGESGEYRAARDR
                     LLAHEIKMRRAMETVAAARRELPPGGVVPEDYVFQDASRDCSELLYAPPEPGQDPRHV
                     GTIEPLWNLFDLTPEQLRYEGNL"
     gene            complement(1401..1541)
                     /locus_tag="VHH53_02330"
     CDS             complement(1401..1541)
                     /locus_tag="VHH53_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289043.1"
                     /translation="MVVLVRGDIELASWPLTNRDRPGLAVVDELARLQLAARSRLLDP
                     AA"
BASE COUNT          237 a          577 c          551 g          281 t
ORIGIN      
        1 gcctctcggc gtagcatgga cgctcgtgcc catccgttca gatactgtcg gcagcagcga
       61 ggtccggtcg gaggacctgg agcagcaccg ccgggagctt actggttact gctaccggat
      121 gctcggctcg ttctttgagg ctgaggacgc cgtgcaggag acgatggtgc gggcctggcg
      181 gggtatcgac ggctttgaag gccggtcggc cctgcggtcg tggctttacc gcatcgccac
      241 caatgtgtgt ctcgacatgc tgcggggccg cgagcgtcga gctcggccga tggacctggg
      301 cccgtcctca acggcagagg gatctctggg gccggtactt cccgagcacg cctgggtgca
      361 acctgtcccg gacgctcgcg ttctgcccgg cgacagtgac cccgccgagc tggcggtgtc
      421 gaaagagagc gtccgcctgg cattcgtcac cgccctgcag cacctccctg ccaagcagcg
      481 cgccgtgttg atcctgcgcg aagtgctgcg ctggcacgcg accgaggtgg ccgagctgct
      541 cgagaccagc gtggcgtcgg tcaacagtgc actgcagcga gcccggtcca ccctggcggc
      601 ccgcgacgcc agtgaggtca gcgctttctc gcccgcggtg aacgccgacc agcgagcgct
      661 gcttgcccgc tacgtggacg ccttcgagcg ctacgacatc acctccctgg tgtcgctgct
      721 gcacgaggac gcggtcatgt cgatgccgcc ctacgacttc tggctgcgcg ggccagtgga
      781 gatgggccgg tggttcctgg gccagggctg cgggtgccgt ggctcacgcc tggtaccgac
      841 ggcggctaac ggctgcgccg cgttcgggtc ctaccgcccg gaccccgacg gtggccaccg
      901 gccgttcgcc ctgcaggtca tcgagatctc aggcgaccgg atcgtcgggc accacaactt
      961 cctcgacacc gccctgttcg ccgccttcgg cctcccagac cggcttgagt cataggttgc
     1021 cctcgtagcg cagctgctcg ggtgtgaggt cgaacaggtt ccacaggggc tcgatggtgc
     1081 cgacgtggcg gggatcctgc ccgggctctg gtggcgcgta gagcagttcc gagcagtccc
     1141 gactggcatc ctggaagacg taatcctccg ggacgacgcc gccgggtggc agctcccttc
     1201 tcgcggcagc caccgtctcc atcgcgcgac gcatcttgat ctcgtgggcg agcagccggt
     1261 cgcgtgccgc ccggtactcg cctgattcgc cgggaaacgt gatgcccatg ttcctggtcc
     1321 tctcggtcag gccggcgata tcgctgacca cctcgcccag gccaagcagg tcgagcagct
     1381 ctagcaactc gacgcaggca tcacgcagcc ggatcgagca accgcgaccg ggcggcgagt
     1441 tgcaaccgcg ccagctcgtc caccacggcg aggccgggac ggtcccggtt tgtcagcggc
     1501 cagctcgcca gctcgatgtc cccgcggacg agcaccacca tggcgccggt gtcttctcgc
     1561 cgcacactcc tacgacgacg gcaggctggc gaactcatcg gtgcgcgagc caatgagttc
     1621 cgcctcgcct cgccgtctca cctagc
//
LOCUS       DATADN010000177         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1222851, whole genome shotgun sequence.
ACCESSION   DATADN010000177 DATADN010000000
VERSION     DATADN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222851"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /gene="hypD"
                     /locus_tag="VHH53_02335"
     CDS             <1..1082
                     /gene="hypD"
                     /locus_tag="VHH53_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEX2289044.1"
                     /translation="YKVMEVCGGHTHAIYKYGLDDLLPANVELVHGPGCPVCVIPMGR
                     VDDAIHIASQPGVIMTSFGDMMRVPGGRGSFFDSSAAGTDIRMVYSPLDALKIARQNP
                     DKHVIFMAIGMETTAPSTAMTVLRAASEGIENFSIFCNHVMIVPAIKAILDSPDLRLD
                     GFIGPGHVSTVIGCRPYEFIASEYGKPLVTAGFEPLDILQSVYMLLQQLAEGRCEVEN
                     QYSRVVPWDGNTVALRAVNEVMELRPFFEWRGLGFISHSALRVRERYAAFDAERHFEL
                     PGVRVADPKACQCGEVLKGVLKPWECKVFGTACTPETPIGTCMVSSEGACAAYYNFGR
                     FTRERVREATEVHQIEMHKVKASRA"
     gene            1079..2263
                     /gene="hypE"
                     /locus_tag="VHH53_02340"
     CDS             1079..2263
                     /gene="hypE"
                     /locus_tag="VHH53_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEX2289045.1"
                     /translation="MTQYQDTTQQHRADRAQAAVAEHAAGQPGHAPAGTEHTGREQLV
                     LERIERARRARPRVREKLVTLAHGAGGKATQSLIEAVFLDAFRNPQLEPLEDAATVVA
                     GDARLAFTTDSYVVSPLFFPGGDIGDLAVNGTVNDLAVSGARPAHLSAGFILEEGFPV
                     EDLRRIVASMAAAAAAAGVSIVTGDTKVVQRGKGDGCYINTAGVGLLTDNLTLGVATA
                     RPGDAVIVSGPVGDHGMTIMLARGELDLEADLVSDTAPVHGLVRGLLDAVGPDVRAMR
                     DATRGGVATILNEVATAAGVAVVVEEEAVPVRAEVRGACELLGIDPLYVACEGRIVVI
                     VDPGAADAAVAALQAHPLGAGATVVGRVADDPPGLVLLKTGFGGTRIIDLLVGDPLPR
                     IC"
     gene            2300..2590
                     /locus_tag="VHH53_02345"
     CDS             2300..2590
                     /locus_tag="VHH53_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289046.1"
                     /translation="MIAEIVADWPESGELVYIERRGDSYTLRRIGADGAVPVPTPGES
                     LPDAWIYYGGWQAQHRDDVSAFFEDLFAEMEAAAGGHDRCRWSADDPWPHAH"
     gene            2610..>3564
                     /locus_tag="VHH53_02350"
     CDS             2610..>3564
                     /locus_tag="VHH53_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289047.1"
                     /translation="MAYRIHASDRTAFKCCRRQWDLSSPNRQNLEPISRSDRIDLNQA
                     LRDALAVYYFPGMWEWDRAIVQPLVHQALERSVRGQSESAADSGADEQAAVLARGHAL
                     LDAYVTWAPTVDHFTPIRVETDFEVNIPDPAAPDTTLLTPDGEEVRYCDRVDLLAIDS
                     DDAYWVIQHRLVADGWTNHDDLQLDERTIAWCWAWPLFYLGMKITGTIYNEIRADARE
                     PKTGEPGAVVPQQVRYQQVGHRRMYARSSTVTGERLHAEGTDMFRRTRIRRDAAELAV
                     AGAGLGAEARTATSAGLDLYPNPEPGICAHCSYRSPCLALNH"
BASE COUNT          580 a         1122 c         1237 g          625 t
ORIGIN      
        1 actacaaggt catggaggtc tgcggcggcc acacccacgc gatctacaag tacgggctcg
       61 acgacctgct cccggccaac gtcgagctcg tgcacgggcc cgggtgtccc gtctgcgtga
      121 tcccgatggg ccgcgtggac gacgccatcc acatcgcctc acagcccggt gtgatcatga
      181 cgtcgttcgg cgacatgatg cgggtgcccg gcgggcgcgg gtcattcttc gactcctcgg
      241 ctgccggcac cgacatccgg atggtctatt cgccgctcga cgcgctgaag attgcccggc
      301 agaacccgga caagcatgtg atcttcatgg cgatcgggat ggagaccacc gcgccgtcta
      361 ccgcgatgac cgtgctgcgg gcggcgtctg agggcatcga gaacttctcg attttctgta
      421 accatgtcat gatcgtcccg gcaatcaagg ccatcctgga ctctcccgac ctgcggctgg
      481 atggattcat cggtccgggc cacgtctcga ccgtcatcgg ctgccggccc tatgagttca
      541 tcgcctccga gtacggcaag ccgctggtca cggcggggtt cgagccactg gacatcctgc
      601 agtcggtgta catgctgctg cagcagctgg ccgagggccg ctgcgaggtg gagaaccagt
      661 actcccgggt ggtgccgtgg gatggcaaca cggtggccct gcgcgcggtc aacgaagtga
      721 tggagctgcg gcccttcttc gaatggcgtg ggcttggttt catctcgcac tcggctctgc
      781 gggtgcggga gcgttacgcg gctttcgacg cggagcgaca cttcgagcta cctggcgttc
      841 gagtagctga cccgaaggcg tgtcaatgcg gtgaggtgct caagggcgtg ctcaagccct
      901 gggagtgcaa ggtgttcggc acggcatgta cgccggaaac accgatcggg acttgcatgg
      961 tctcctccga gggagcctgt gcggcgtact acaacttcgg ccggttcacc cgagaacggg
     1021 tccgtgaggc tactgaggta catcagatag agatgcataa ggtcaaggcg agccgggcat
     1081 gacccagtac caggacacca cccagcagca ccgagcggac agggcgcagg cagccgttgc
     1141 cgagcacgcc gccgggcagc ctgggcatgc cccggccggg acagagcaca ccggccggga
     1201 gcagttggtg ttggaacgca tcgagcgcgc ccggcgggcc cggccccggg tgcgtgagaa
     1261 gctcgtcacg ctcgcgcacg gcgccggcgg caaggccacc cagtcgctga tcgaggcggt
     1321 cttcctggat gctttccgca atccccagct ggagccgctt gaagacgccg cgacggtggt
     1381 cgccggtgat gcgcggctgg ctttcaccac ggattcctat gtggtgtcgc cgctgttctt
     1441 cccgggcggc gacatcggtg accttgcggt caacggcaca gtcaacgatc tggccgtctc
     1501 cggggcacgg ccagctcatc tgtccgcggg tttcattctg gaagagggct ttccggtcga
     1561 ggacctgcgg cggatcgtgg cgtcgatggc cgcggccgct gcggcggctg gggtctcgat
     1621 cgtcaccggc gacaccaagg tcgtgcagcg gggcaagggc gatggctgct acatcaacac
     1681 cgccggggtg gggttgctca ccgataacct gaccctcggg gtggccactg cccgtcccgg
     1741 tgacgcagtg atcgtctcag ggccggtggg cgaccacggg atgaccatca tgctggcgcg
     1801 cggtgagctg gacctcgagg ccgacctggt ctctgacacc gcgccggtgc atggcctggt
     1861 gcgggggctg ctcgacgcgg tgggtccaga cgttcgggcg atgcgcgacg ccacgcgtgg
     1921 tggcgttgcg accatcctca acgaagtggc cacggctgcg ggcgttgccg tggtggtcga
     1981 ggaggaagca gtcccggtac gcgcagaggt ccgcggggcc tgcgagctgt tgggcatcga
     2041 cccgctctac gtcgcctgcg agggacggat cgtggtgatc gtcgatcccg gcgcggccga
     2101 cgccgcagtg gcagcactgc aggcccatcc gctcggcgcc ggggccaccg tggtcggccg
     2161 ggtagccgac gacccgccgg ggctggtgct gctcaagacg ggattcgggg gcacccggat
     2221 catcgacctg ctggtcggcg acccgctgcc gcggatctgc tgagcgcgtt gtcagggagg
     2281 ttcgctgaac cggaatgagt tgatcgctga gatcgtggct gattggcccg aatccggtga
     2341 gcttgtgtac atcgaacgtc ggggcgacag ttacacgttg cgacgcatcg gcgccgatgg
     2401 agccgtcccc gtgccgacgc ccggtgagtc gctgcccgat gcctggatct actacggtgg
     2461 gtggcaggca cagcatcgag acgacgtgtc ggcgttcttc gaggatctgt tcgccgagat
     2521 ggaagcagcg gctgggggtc atgatcgttg ccgctggtca gcggatgacc cctggccgca
     2581 cgcgcattaa gtcactgagt tgaggtgcga tggcctaccg catccacgcg tctgaccgga
     2641 cggcgttcaa gtgctgccgc cggcaatggg atctgagctc gcccaaccgg cagaacctcg
     2701 aaccgatctc ccggtcggat cgtatcgacc tgaaccaggc actgcgtgat gcgcttgcgg
     2761 tgtactactt ccccgggatg tgggagtggg acagggccat tgtgcaaccg ctggtgcatc
     2821 aggctctgga acggtcggtg cgcggccaat ccgaatctgc ggccgatagc ggggccgacg
     2881 aacaggcggc ggtgctggcc aggggacatg cgttgctcga tgcttacgtg acctgggcgc
     2941 cgacggtaga tcacttcacc ccgattcgcg tcgagaccga ctttgaggtg aacattcccg
     3001 atccggcagc gcccgacacc actctgctga ccccggacgg ggaggaggtt cgctattgcg
     3061 accgggtcga tctgctcgcc atcgattccg atgacgccta ctgggttatc cagcaccggc
     3121 tggtagctga cggctggacc aatcacgatg acctgcagct cgacgagcgg acaatcgcgt
     3181 ggtgttgggc gtggccgctg ttctacctcg ggatgaagat cacaggcacg atctacaacg
     3241 agatccgcgc tgacgcgcgc gagccaaaaa cgggcgagcc gggcgctgta gtcccgcaac
     3301 aagtgcggta tcagcaggtg ggtcaccggc ggatgtatgc ccgcagcagc actgtcactg
     3361 gtgaacgcct gcatgccgag ggcaccgaca tgttccgccg tacccgtatc cggcgcgacg
     3421 cggccgagct ggcggtcgcc ggcgcaggcc tgggcgctga ggcacgcacc gccacgagcg
     3481 caggtctcga tctctatccc aacccagagc ctgggatttg cgcacactgc tcctatcgct
     3541 ccccctgctt ggcgctcaac cacg
//
LOCUS       DATADN010000178         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_17752, whole genome shotgun sequence.
ACCESSION   DATADN010000178 DATADN010000000
VERSION     DATADN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17752"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHH53_02355"
     CDS             <1..198
                     /locus_tag="VHH53_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289048.1"
                     /translation="GMTWHQAAAVGAMLSCGGLITLAMALVAQQAGTITQPMHIAFVI
                     AAIVTTLLTGPLLDMFTRRDA"
     gene            209..877
                     /locus_tag="VHH53_02360"
     CDS             209..877
                     /locus_tag="VHH53_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2289049.1"
                     /translation="MSRRTEGPLRVVVVDDHQMVLNGLRAMLAAYSGQVEIVGEATEP
                     ELAMKLVIEHEPDITLLDVRLRGSSGMDLCAEIRQRQPGCKVVFLTVYDDEQYLYQAL
                     RLGAAGFLLKRVRGAELVDHLQRIHQGEILIDPSLAAKVAMSVAHLRGGEFWPGAHLG
                     LTQRESEVLALMVAGLSNRAIAGRLTVSEETVKTHARGIYRKLDVPDRSGAVAAALRE
                     GVFQ"
     gene            874..>1616
                     /locus_tag="VHH53_02365"
     CDS             874..>1616
                     /locus_tag="VHH53_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2289050.1"
                     /translation="MIHSRLGLADAASDAQLLHRVIGILSAGLDLDVVVQRVADLITA
                     TTSTDVCFVHLLDKPRGRLQLRGATPPFDQFAGRIELPVGDGVTGWVAAHCAPAVITD
                     NKRADPRYLYIPELRGEDFTSMASVPMITRPGDLVGVLNVHTHDRREFTEADVELLGT
                     VAGLMAGAIENASLHRKLAEREEAMERFAEQIVLLQETERRRLAGEIHDGISQRIVSL
                     SFHLSAAADAVASDPAGAADQIARAQELAA"
BASE COUNT          262 a          537 c          541 g          276 t
ORIGIN      
        1 ggcatgacat ggcatcaagc ggccgccgtg ggggcgatgc tcagctgcgg cggcctgatc
       61 acgcttgcga tggcgctggt cgcccagcaa gcgggcacga tcacccagcc gatgcacatc
      121 gcgttcgtga tcgccgcgat cgtcacgacc ctgctcaccg gtccgctgct ggacatgttc
      181 acccgccgcg atgcgtaggc tgcttttcat gagcaggcgg actgaggggc cgctacgcgt
      241 cgtcgtcgtg gatgaccacc agatggtgct caatggactg cgcgcgatgc tggctgccta
      301 ctccggccag gttgagatcg tcggtgaggc caccgagccg gaactcgcga tgaaactggt
      361 catcgagcac gagcctgaca tcaccttgct cgatgtccgg ctacgcggat ccagcggcat
      421 ggatctgtgc gcggagatcc gtcaacgtca gcctggctgc aaggtcgtgt tcttgaccgt
      481 atacgacgac gagcagtatc tctatcaggc gctacggctc ggcgccgccg gcttcctgct
      541 caagcgggtg cggggcgccg agctggtgga tcacctgcag cgaatccacc agggcgagat
      601 cctgatcgat cccagcctgg cagcaaaggt tgcgatgtcg gtagcgcatc tgcgcggtgg
      661 ggagttttgg ccgggagccc atctcgggct gacccagcgc gagagcgagg tgctcgcgct
      721 catggtggcc gggttgtcca accgtgccat cgccggaagg ctcaccgtca gcgaggagac
      781 cgtcaaaacc cacgcccgcg ggatttaccg caagctcgat gtcccggacc gctccggtgc
      841 cgtcgccgcg gcgctgcggg agggggtatt ccagtgatcc actcgcggtt gggtctggcg
      901 gatgccgctt ctgatgccca gctgctgcac cgggtgatcg ggatcctgtc ggcaggtctg
      961 gatctcgacg tggtcgtcca gcgagtggcc gacctgatca ccgcgaccac ctccaccgac
     1021 gtctgcttcg tccatctgct ggacaaaccg cgcgggcggc tgcagctacg cggcgccaca
     1081 cctcccttcg accagttcgc cgggcggatc gagctgccgg tcggtgacgg ggtgaccggc
     1141 tgggtcgccg cccactgcgc gccggcggtg atcaccgaca acaagcgcgc cgatccgcgc
     1201 tacctctaca ttcccgagct gcgcggggaa gacttcacgt cgatggcctc ggtgccgatg
     1261 atcacccgac ctggcgatct ggtgggtgtg ctcaacgtgc atacccacga ccgccgcgag
     1321 ttcaccgagg ccgacgtcga gctgctgggc accgtggcgg gtctgatggc cggtgccatc
     1381 gagaacgcca gcctgcaccg caagctcgcc gaacgagagg aggcgatgga gcggttcgct
     1441 gagcagatcg tgctgctcca ggagaccgag cggcgacgcc ttgccggcga gatccacgat
     1501 ggaatcagcc agcgcatcgt cagcttgtcg tttcacctct cagcagcggc agacgcggtt
     1561 gcctccgacc cagccggcgc cgccgaccag atcgctcgcg cccaggaact cgccgc
//
LOCUS       DATADN010000179         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_322868, whole genome shotgun sequence.
ACCESSION   DATADN010000179 DATADN010000000
VERSION     DATADN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322868"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VHH53_02370"
     CDS             complement(<1..1276)
                     /locus_tag="VHH53_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2289051.1"
                     /translation="MSTSAEQEAGSPRALQPVHGQPPAPGRAPDGTGSPLRRHRSSRV
                     IAGVSSGIAEHLGVPVLWVRLVFAVLAGLGGAGLVGYALLWAFVPAIETGAGRPGTAR
                     ERQQAVGLVVLGAGLALAGAALDTGWGGWVTGPLGIALVGAGVVWREADAGQRTRWRS
                     GAGVLFGGGRMALLRVLAGIALVTSGIAVFLLNQDNLGQLRFALLAAAATLVGVVVLT
                     VPWWLRLMRALGEERRARIRTEERAEIAAHLHDSVLQTLALIQQQSDSAREVRRLARG
                     QERELRSWLYGPRGYGRPVSNGAGGAAVIAFAATLNAAAGEVEDTYAVGVQVVVVGDC
                     VLEEPLTALVQASREAMVNAAKHAGVAEVSVYAEVEPGHVHVFIRDRGIGFDPAAVAD
                     DRHGLADSIRGRMERHGGTVTLRTAPGEGTEVQ"
     gene            1403..>1756
                     /locus_tag="VHH53_02375"
     CDS             1403..>1756
                     /locus_tag="VHH53_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2289052.1"
                     /translation="MSEINVPRTASDFWATRPHRRSDDRKIGGVAAAIGRRYAIDPVL
                     VRVAFIVATMFSGMGLLMYLLGWLLLPAEGDRVSAAESLLGRGRSSMSPVLTVVLMLL
                     LFVVTAGVFDGPPSGV"
BASE COUNT          282 a          653 c          576 g          245 t
ORIGIN      
        1 attgcacttc ggtgccctcc ccgggtgcgg tgcgcagggt caccgtgcca ccgtggcgtt
       61 ccatcctgcc gcggatcgag tccgccaggc cgtgccggtc gtcggcgaca gcggcagggt
      121 cgaatccgat gccccggtcc cggatgaaga catgcacgtg cccgggttcg acttcggcat
      181 acacactgac ctcggcgacc ccagcgtgct tggctgcgtt gaccatcgct tcccggctgg
      241 cttgcaccag cgctgtcagc ggctcctcga ggacacaatc gcccacgacg acgacctgga
      301 cacccaccgc gtacgtgtcc tccacctcgc cggcagcggc gttgagagtc gccgcgaacg
      361 cgatcaccgc agcgccaccg gcaccgttgg acaccgggcg tccgtagccg cggggaccgt
      421 agagccagga acgaagctcc cgctcctgac cccgggccag ccggcgaacc tcccgggctg
      481 agtcggactg ctgctggatc agggccaggg tctgcagcac cgagtcgtgc aggtgcgcgg
      541 cgatctcggc ccgttcctcg gtgcggatcc gggcccggcg ttcctcgcct aacgcgcgca
      601 tgagccgtaa ccaccacggc acggtgagca caaccacacc gaccagggtc gcggccgcgg
      661 cgaggagcgc gaaccggagc tgacccaggt tgtcctgatt gagcaggaac accgcgatcc
      721 cgctggtgac cagcgcgatc ccggccaaca cccggagcag ggccattcgc ccgccgccga
      781 acaacacccc cgcgccactg cgccagcggg tccgctggcc ggcgtcggct tcccgccaca
      841 ccacacccgc accgaccagg gcgatcccca gcggaccggt cacccagcca ccccacccag
      901 tgtccagggc ggcaccggcc agtgccagac ccgcgccgag cacgaccagg ccgactgcct
      961 gctgccgctc ccgcgcagtg cccggccggc cggcaccagt ttcgatcgcc ggtacgaaag
     1021 cccacagcag cgcatagccc accaacccgg ccccaccgag gccggcgagc accgcgaaga
     1081 ccagccgcac ccacagcacc ggcacaccaa gatgctcggc gatcccgctg gacaccccgg
     1141 cgatgacccg gctggaccga tgccgacgca gtggtgaccc ggtaccgtcc ggggctcggc
     1201 ctggcgccgg aggttgccca tgcaccggct ggagggcccg gggactgccg gcctcctgct
     1261 ccgccgatgt gctcatgcgc atcatcgtca cacgtccggc agatcggggg tatcagggtc
     1321 gaccccggta tccctcaacc cggggacgga tcagggtgac cccggatggt gcttgacgtc
     1381 ctcgcaggcc aagctcaagg gcgtgagcga gatcaacgtg ccgcgcactg ccagcgactt
     1441 ctgggccacc aggccgcatc gccgaagcga cgaccgcaag atcggcggag tcgcagcggc
     1501 gatcgggcgc cggtatgcca tcgacccggt attggttcga gtggcgttca ttgtggccac
     1561 catgttcagc gggatggggt tgctgatgta cctgctcggc tggttactgc tgccagccga
     1621 gggagacagg gtctccgccg cggagagtct gctcggacgg ggccgcagct ccatgtcgcc
     1681 agtgctcaca gtggtgctca tgctgctgtt atttgtggta accgcgggcg tgttcgacgg
     1741 gccgccctcc ggcgtg
//
LOCUS       DATADN010000180         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_775022, whole genome shotgun sequence.
ACCESSION   DATADN010000180 DATADN010000000
VERSION     DATADN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775022"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..739
                     /locus_tag="VHH53_02380"
     CDS             317..739
                     /locus_tag="VHH53_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289053.1"
                     /translation="MAQNLMVWLIERRVVLDERAAQLRKQLADTEAEVARLGAAEVVY
                     QQYQEDAEAGHPDAAAWQQALEEATGGTPVAPPRPVGTPGAAGQLLIPHHGPGTGTDD
                     LPADYQKIMEIVGSLRPTCHNGRFGPQRKRPRRELVHA"
     gene            823..1572
                     /locus_tag="VHH53_02385"
     CDS             823..1572
                     /locus_tag="VHH53_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2289054.1"
                     /translation="MADLIRAHRKQIRSRWRKLDAGQIALIVLAHLRNDERLADLAGG
                     NAVSASTVRRWVLEVLGLLAARSPRLDRALAKVAKDSGCVVLLDGTLIRTRRRTGRAN
                     RKNYSGKHKAHGLLFLALTDAKGRLLWISSARRGACSEITAARYEKLCARLRELELAA
                     VADLGFVGLDEGAEEQPAVITGFKASGGKRLSSPKKVVNRLISSLRAPVEHGFASLKT
                     FRILTKVRMHPRHATALVRALLVLTHHQVAR"
     gene            complement(1637..>1938)
                     /locus_tag="VHH53_02390"
     CDS             complement(1637..>1938)
                     /locus_tag="VHH53_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2289055.1"
                     /translation="TIKTIYAVTSLTPEQATPAQLAELIRGHWQVEALHHVRDVTFAE
                     DASRIRTGTAPRAMATLRNLAIGLIRQAGWTNIAAATDHYRSRTDHALQLLDLET"
BASE COUNT          302 a          639 c          692 g          305 t
ORIGIN      
        1 acaaaccaga tcatcgcagg tcgaggcact ctccttcgcc tacctcacac tccgtcacca
       61 gtaaccgccc gcatggtgaa ttcatgaggc cagtgagcct ttcccagtga ggaagtttca
      121 tcgtgattcg tgcaggtcaa caaccctggt gtgacgggcg gttgggctgc tcgcgcatgt
      181 cacgagcgtg atcgcgtcgg ggagatcatc tgtcggcccg cttcgggtcg tcttcctcgg
      241 atctcctgca cgctgcgcac tggtacccct accgtgcgtg cagggtcgcg acggcgggcc
      301 cgcgaaggga ggggacatgg cccagaatct gatggtgtgg ctgatagagc gccgggtggt
      361 gctggatgaa cgcgccgcgc agctgcgcaa gcagttggcc gacaccgagg ccgaagtcgc
      421 ccggctcggg gccgccgagg tcgtctacca gcagtaccag gaagatgccg aggccgggca
      481 ccctgacgcc gcggcctggc agcaggccct ggaagaggcc accggtggca cgcccgtggc
      541 gcccccgcgg cccgtcggca cgcccggagc cgctggccag ctgctgattc cccaccatgg
      601 gcccggcacc ggcacggacg acctgcccgc cgactaccag aagatcatgg aaatcgtcgg
      661 tagcctgcgg ccgacctgcc acaacggccg attcgggccg caacgcaagc gcccccggcg
      721 cgagttggtc catgcgtgaa caagaagacc agccccgccg agggcgacac cggccttgtc
      781 tactccgtcc gcctgccgct gtccagcgcg accgtcaacc gcgtggccga cctgatccgc
      841 gcccaccgca aacagatccg ctcccggtgg cgcaagctcg acgccgggca gatcgcgctg
      901 atcgtgctgg cccacctgcg caacgacgag cgcctggccg atctggccgg cgggaacgcg
      961 gtctcggcct ccacggtgcg gcgctgggtg ctggaggtgc tcggcctgct ggccgcacgt
     1021 tccccccgcc tggaccgggc gctggccaag gtggccaagg acagcggctg cgtggtcctg
     1081 ctggacggga cgctgatccg cacccggcgg cgcaccgggc gggcgaaccg gaagaactac
     1141 tcggggaagc ataaggcgca tggcctgctg ttcctcgccc tgaccgacgc gaaggggcgg
     1201 cttttgtgga tctcctcggc ccgccgcggc gcctgcagtg agatcacggc ggcccgctac
     1261 gagaagctgt gcgcgcgact gcgcgagctg gagctcgcag cggtggccga cctggggttc
     1321 gtcggtctgg acgagggcgc cgaggagcag ccggcggtca tcacggggtt caaggcgagc
     1381 ggcggcaagc gcctgtcgtc accgaagaag gtcgtgaacc ggctgatcag cagcctgcgg
     1441 gcgccagtcg agcacggctt cgcgagcctg aagaccttcc ggatcctgac caaggtgcgc
     1501 atgcatccgc ggcacgcgac cgcgctggtg cgggccctgc tggtgctgac gcaccaccag
     1561 gtggctcgat gaccgatgat ctccccacaa tgatcaccgt gatgagctgc gcgagcaccc
     1621 cagggctgat gcattctcag gtttcaagat cgagtagttg gagtgcgtgg tcggttcgtg
     1681 accggtagtg gtcggtggcg gcggcgatgt tcgtccagcc ggcttggcgg atgaggccga
     1741 tggcgagatt gcggagggtc gccatcgccc ggggtgcggt gccggtgcgg atgcgagagg
     1801 cgtcctcggc gaaggtcacg tctctcacgt ggtgcagggc ttccacctgc cagtgcccgc
     1861 ggatcagttc cgcgagctgg gccggggtgg cctgttccgg ggtgaggctg gtgaccgcgt
     1921 agatcgtctt gatggtgg
//
LOCUS       DATADN010000181         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_622634, whole genome shotgun sequence.
ACCESSION   DATADN010000181 DATADN010000000
VERSION     DATADN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622634"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..932)
                     /locus_tag="VHH53_02395"
     CDS             complement(138..932)
                     /locus_tag="VHH53_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX2289056.1"
                     /translation="MLPTDPAALPLLHDGRIDITGRIVDASNATLFCTVHCGGVSAEC
                     VYKPIRGERPLWDFPDGTLAGREVASWLLSEATGWCLVPPTVLRNGPLGPGMVQLWVD
                     TEDERDLVDVVAIDAVPAGWRPVLRVQGRYGNPALLVHADTEGVRRLAVLDVLLNNAD
                     RKGGHVLAGLDGAVYGVDHGICLHHEDKLRTVLWGFLGEPLSADMLAVLRRVRSELDG
                     GLGAALAEHLTRTELKALQKRIDRLLITETFPAPNGSWPAIPWPAF"
     gene            complement(944..1513)
                     /locus_tag="VHH53_02400"
     CDS             complement(944..1513)
                     /locus_tag="VHH53_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEX2289057.1"
                     /translation="MSRVIHIFRTPERFIAGTVGEPGDRTFYLQAIDDAKVISVQLEK
                     EQVAVLAERLSALLDEVARRLGIDTDTTESGEHAVDAEPLRTPVEQEFRVGTMGLGWD
                     VESHAIVVELLAVTEHELDESVVLDDTEEGPDAVRVFLSLPQARAFATRAERVITAGR
                     KPCPLCTEPLDPKGHICPRQNGYRRQVEA"
BASE COUNT          268 a          606 c          539 g          243 t
ORIGIN      
        1 ggtggcgccc gtgaaaccgc cttctcgcca caaccgcaga aactgcggcg gaccgggcaa
       61 cggtgcgctg acccgatcgg cgaacgagcg cggccaccgc ctgcgcggtg ccgacgtttc
      121 gacccagcga gcaggtctca aaacgccggc cacgggatcg ctggccagga cccattgggc
      181 gccggaaacg tctcggtgat cagcagccgg tcgatccgct tctgcagcgc cttcagctcc
      241 gtacgggtca ggtgctcagc cagcgcggcg cccagcccgc cgtccagctc ggatcgcacc
      301 cggcgcagca ccgccagcat gtcggccgac aacggctcac ctaggaagcc ccacagcacg
      361 gtacgcagct tgtcctcgtg gtgcaggcag atcccgtggt ccaccccgta caccgcgccg
      421 tcgagcccgg cgagcacgtg cccacccttg cggtcggcgt tgttgagcag cacgtcgagc
      481 accgccagcc ggcgcacccc ttcggtgtcg gcatgcacca gcagcgccgg attgccgtac
      541 cgaccctgta cccgcaacac cgggcgccag cctgccggca ccgcgtcgat cgcgaccacg
      601 tccaccaggt cgcgttcatc ctcggtgtcc acccagagct gcaccatgcc gggtcccaac
      661 gggccgtttc gaagcaccgt gggcggtacc aagcaccagc cggtggcctc ggacagcaac
      721 cagctggcca cctcacgacc agccagggtg ccgtccggga agtcccagag cggacgctcg
      781 ccgcggatcg gcttgtacac gcactcagcg ctgaccccgc cgcagtgcac cgtgcagaac
      841 aaggtggcgt tcgatgcgtc cacgatccgg ccggtgatgt cgatgcggcc gtcgtgcagt
      901 aacggcagcg ccgccggatc ggtgggcagc acggccgacc cgttcaggcc tcgacctggc
      961 gacggtagcc attctgccgt gggcagatgt gccctttggg gtccaacggc tccgtgcaca
     1021 gcgggcacgg tttgcgcccc gcagtgatca cccgctcagc acgggtggcg aatgcccggg
     1081 cctgcgggag cgacaggaac acccgcaccg cgtcgggacc ctcttcggtg tcgtcgagca
     1141 ccaccgactc atccagctcg tgctcggtca cggcgagcag ttcgacgacg atggcgtgcg
     1201 attccacgtc ccagccaagt cccatggtgc cgacccggaa ctcctgctcg acgggcgtgc
     1261 gcagcggctc ggcatccact gcgtgctccc cggactctgt ggtgtcggtg tcgataccca
     1321 aacgccgagc cacctcgtcg agcagcgcgg acagccgttc ggcgaggacc gcgacctgct
     1381 ccttctccag ctgcacgctg atgaccttgg cgtcgtcaat ggcctgcagg tagaaggtcc
     1441 ggtcccctgg ctcgccgacc gtgcccgcga tgaaccgctc gggggtgcgg aagatgtgga
     1501 tgacacgaga catgacagtc tcgacactag agcacgctga gctgtccggc agcagccctc
     1561 ccctgctcag ccggtgccgg tgggcccgcc gggggtagcg tccgacgagg gtcgccgcct
     1621 acgccggcgt ttgggcggga ccagtgactg taagtc
//
LOCUS       DATADN010000182         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_775199, whole genome shotgun sequence.
ACCESSION   DATADN010000182 DATADN010000000
VERSION     DATADN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775199"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VHH53_02405"
     CDS             complement(<1..533)
                     /locus_tag="VHH53_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289058.1"
                     /translation="MLALGVTGCHSGSGTAGASGASPAPKATPAQPAPKDVLVAATAK
                     LAKTRYKFTFDPGCSTTVSGVVDPQRKSSQVEERSPGRDTTMDMSLLSVDSQAWLKVN
                     DEWMNVRGQTINSPLTGKWLHLDPAKIHGAEIPVMRDFFGGPAGPAGEAFQNVRDVQK
                     AGDDTYTGRFDMSKTDNL"
     gene            complement(1157..1608)
                     /locus_tag="VHH53_02410"
                     /pseudo
     CDS             complement(1157..1608)
                     /locus_tag="VHH53_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          237 a          518 c          543 g          310 t
ORIGIN      
        1 agattgtcgg tcttcgacat gtcgaacctg ccggtgtagg tgtcgtcgcc ggccttctgc
       61 acgtcgcgga cgttctggaa ggcctcgccc gcggggccgg caggaccacc gaagaaatcg
      121 cgcatcacgg ggatctcggc gccatggatc ttggcagggt ccaggtgcaa ccacttgccc
      181 gtcagtgggc tgttgatggt ctgcccccgg acgttcatcc attcgtcgtt caccttgagc
      241 caggcctgcg agtccacgga cagcaggctc atgtccatgg ttgtgtcccg cccgggggat
      301 ctctcctcga cctgggagct cttgcgctgc gggtcgacca caccgctgac ggtggtggag
      361 cagccgggat cgaaggtgaa cttgtagcgc gtcttcgcca gctttgccgt ggccgcgacg
      421 aggacgtcct tcggtgccgg ctgagcgggg gtcgccttcg gtgcaggcga ggcgcctgac
      481 gccccggccg taccgctacc gctgtggcat cccgtcacgc cgagcgcgag caccgtgacg
      541 gcggcgatac cggcaaccgt tcgcagttcc ctgcgcgcac cgcggaaggc gaccttgtga
      601 tggatgccaa acactacgct ccctctgttc gggtgacggc atgaagccgc tccttaaggc
      661 acaggctgaa tacgcgcgcc ccttctcgtg caccttgtgg ctcttgcagg ttgtgatagt
      721 gcccggctgc gatcccgcct tcgtggcggt gaggcctcgg gggatgtacc gcctcatgac
      781 agccgccgga tcgcgaggta ccgccccgcg tcagtggcaa gccacaacca cagaccgacc
      841 acagcagaag cactcctgct cgccagccgt gcgcccacag cttctcggga tgtaccttgc
      901 acgcgggagg gcgttcgctg ccctttgccc cagccgccag gaaaccttgc ccccgcgacc
      961 cgatccggct ctggtgtttc gtgatccgaa acggctgcct gagggactcg gcagggggtc
     1021 tgtgtccagc caacggatct ggctcacttt cggtggtgac acggcgtggc gctgtcacgt
     1081 tgttgggtgg ataggtcatc tcggcgcggt agtggtgtgc gggcatcaga tggcggcggg
     1141 gtcggaagtg gggtgagatg ccgctgaacg cggacagaaa ccgctgtgct ccgcccacgg
     1201 agcggaagtt tttcatcgac cgttcgcgtt gcctggttgg ctggtggctg ttctccgccc
     1261 ggttgttcag gtacttcgac tgccggtgct cgacgcaagg catgacctcg cggagggccg
     1321 cgccgtagga gcggagcttg tcagtgacga ccacccgcgg cggcgtgcgg gtctgcttga
     1381 ggagcctgcg gaagaagcat cgggctgcgg ccttgtcccg ccggttctgc acgaggatgt
     1441 cgaggacgtt gccgtcctgg tcgacggccc gccacaagta cttcagctca ccgttgatct
     1501 tggtgaagac ctcgtccagg tgccacttgt ccccaagctg gggctgccgg cggcgcagcg
     1561 cgttggcgta aatctgcccg aacttgccgc accaccgccg gaccgtcc
//
LOCUS       DATADN010000183         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1077022, whole genome shotgun sequence.
ACCESSION   DATADN010000183 DATADN010000000
VERSION     DATADN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077022"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1111
                     /locus_tag="VHH53_02415"
     CDS             50..1111
                     /locus_tag="VHH53_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289059.1"
                     /translation="MSSSVATQPSAPRAATPPQRTQRSLVSRVLARPEIGALAAAIAI
                     FAFFYAVAEPFRTLPAFATVLYASSTIGIVAVGVALLMIGGEFDLSSGVAVTTSALAA
                     AMLTYQFSLNMWVGVVVSLLIALAIGFVNGWLVMRTGIPSFLITLSTFFILQGINLGV
                     TKLVTGAVATPNASSLDGFASARAVFAGDVNLGFITLKITVLWWFLFVAVATWLLLRT
                     RVGNWIFASGGNQASARAVGVPVPRVKIGLFMGVAFLAWFLGMHLLFNFSSVQSGLGV
                     GNEFIYIIAAVVGGALLTGGYGSAVGSALGAFIFGMTTQGIVFAGWDPNWFRTFLGVM
                     LLLAVLVNLYVKNYAARRK"
     gene            1114..1644
                     /locus_tag="VHH53_02420"
                     /pseudo
     CDS             1114..1644
                     /locus_tag="VHH53_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1626..1780)
                     /locus_tag="VHH53_02425"
                     /pseudo
     CDS             complement(1626..1780)
                     /locus_tag="VHH53_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          245 a          627 c          601 g          307 t
ORIGIN      
        1 gccgggtcgg cccggctccc cccggggcac ccgcctccct ggagcagtca tgagctcatc
       61 cgtggcgaca cagccgtccg ccccgcgggc cgccaccccg ccgcagcgca cgcagcgatc
      121 actcgtgtcc cgtgtgctgg cccgacccga gatcggcgcg ctggccgcgg cgatcgcgat
      181 cttcgccttc ttctacgcgg tcgccgaacc cttccggacg ctgccggcct tcgccaccgt
      241 gctctacgcc agctccacca tcggcatcgt ggcggtgggc gtcgcgctgc tgatgatcgg
      301 cggcgagttc gacctgtcct ccggcgtcgc ggtcaccacc tcggcgctgg cggcggcgat
      361 gctgacctac cagttcagcc tgaacatgtg ggtcggcgtc gtggtcagcc tgctcatcgc
      421 gctggcgatc ggcttcgtca acggctggct ggtgatgcgc accgggatcc cgagcttcct
      481 gatcacgctg tcgacgttct tcatcctgca gggcatcaac ctcggcgtca ccaagctggt
      541 caccggcgcg gtcgccacgc cgaacgccag ctcgctggac gggttcgcgt ccgctcgggc
      601 ggtcttcgcc ggggacgtga acctggggtt catcacgctg aagatcaccg tgctgtggtg
      661 gttcctgttc gtcgcggtcg ccacctggct gctgctgcgc accagggtcg gcaactggat
      721 cttcgcctcg ggcggcaacc aggccagcgc ccgggccgtc ggcgtcccgg tgccccgggt
      781 caagatcggc ctgttcatgg gcgtggcctt cctggcctgg ttcctcggca tgcacctgtt
      841 gttcaacttc agctcggtgc agtcgggcct gggggtcggc aacgagttca tctacatcat
      901 cgccgcggtc gtcggcggtg cgttgctcac cggtgggtac ggctcggcgg tggggtcggc
      961 cctcggtgcc ttcatcttcg ggatgaccac ccaggggatc gtcttcgccg gctgggaccc
     1021 gaactggttc cgcaccttcc tcggcgtgat gctgctgctc gccgtcctcg tcaacctgta
     1081 cgtcaagaac tacgccgccc ggaggaagtg accatgaccg acaccatcgc cgagcacgca
     1141 gcggcagacc tgcgggccgg cacgccactc gtggagatgg agggcgtcgg caaggcctac
     1201 ggcgccatcc gcgcgctcga gggcatcaac ctgacggtga acgccggtga ggtggcctgc
     1261 gtcctcgggg acaacggggc cggcaagtcc accctcatca agatcatctc cgggttgcac
     1321 gggcacaccg agggcacgct gcgggtcgac ggccaggagc ggcacttcag ttcgccgcgg
     1381 gagtccctgg acgccgggat cgccaccgtc taccaggacc tcgcggtcgt gggcctcatg
     1441 gaggtctggc ggaacttctt cctcggctcg gagctccgca aggggaagta cccgctggcg
     1501 ccgctggaca tcaaggccat gcgcgagatc gccgacaccg agctgcagaa gatgggcatc
     1561 gtcgtcaagg acatcaacca gccgatcggg acgctgtccg gtggtcagcg gcagtgcgtc
     1621 gcgatcgccc gggcggtgta cttcagcacc acgccggact gcttgacgcc cagcgctgcc
     1681 gtcggctcgt cgaggatgag cacgcgcgcg ccgaagtaga ccgctcgcgc gatcgccacg
     1741 cactgccgct ggccaccgga gagggtgccg atcggctggt
//
LOCUS       DATADN010000184         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_172271, whole genome shotgun sequence.
ACCESSION   DATADN010000184 DATADN010000000
VERSION     DATADN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172271"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VHH53_02430"
     CDS             <1..929
                     /locus_tag="VHH53_02430"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HEX2289060.1"
                     /translation="NELKARARALWSADRRSRCRMNSSAALRVTVRVPQRGDKRALHE
                     TVTRNAAEEFIRHRLRRSADHNARARALSSLQDALGLPEAPLRIECFDMSHIQGTDYV
                     GSMVVMEDGLPKKSEYRRFKVKAVAGNDDYAAMEEVLTRRLTALLAERDQPLTSERPR
                     KFSYPPQLLLVDGGKGQLSVAVRVVEELGLDDEIPVAALAKQFEEVYLPGRADPVRIP
                     RQSEALYLLQRVRDEAHRFAVAYHRQLRGKRMTKSVLDDIPGLGPARRKRLLKELGGV
                     GAVKAASLETLQALPWLPDPVARALHSKLHSA"
     gene            1130..>1526
                     /locus_tag="VHH53_02435"
     CDS             1130..>1526
                     /locus_tag="VHH53_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289061.1"
                     /translation="MIDYSVPPVIDVAYVERVMEALDRVYGDAIRTLARERKITQEFL
                     ERLAAIYGEGQFDRTQAIWVRDVASGLSGLAPTPGDPRTVIQEVMRGEPSCVVLRVDR
                     DFSATHAGPDAASPQEFVGLIPKPLARDPK"
BASE COUNT          237 a          493 c          547 g          249 t
ORIGIN      
        1 gcaatgagct caaagcccgc gcccgcgcat tgtggtcggc ggaccgccgc agccggtgcc
       61 gtatgaactc ctcggccgca ttgcgggtga cggtccgggt gccccagcgg ggcgacaagc
      121 gggcgctgca cgagacggtc acccgcaatg cggccgagga gttcatacgg caccggctgc
      181 ggcggtccgc cgaccacaat gcgcgggcgc gggctttgag ctcattgcag gacgcattgg
      241 ggctgcctga agcaccgcta cgaatcgagt gcttcgacat gtcgcacatc cagggaaccg
      301 attacgtcgg ctccatggtg gtgatggaag acgggctgcc gaagaagtcg gagtaccggc
      361 ggttcaaggt gaaagcggtt gccggcaacg acgactacgc cgctatggaa gaggtgctga
      421 cccggcggtt gaccgccctg ttggccgagc gcgaccagcc gttgacctcg gagcggccgc
      481 gaaagttctc gtacccgccg cagttgctgc tggtcgacgg gggcaagggg cagctgtcgg
      541 tggccgtgcg ggtggtggag gagctggggc tcgacgacga gatccctgtg gctgcgctgg
      601 ccaagcagtt cgaggaggtc tacctgcccg ggcgggccga cccggtgcgc atcccccggc
      661 agtcggaggc gctgtacctg ttgcagcggg tgcgcgacga ggcccaccgg ttcgccgtgg
      721 cgtaccaccg gcagctccgg gggaagcgga tgaccaagtc ggtgctcgac gacatccccg
      781 ggctcgggcc tgcccgccgg aagcggctgc tgaaggagct cggcggggtg ggggcggtca
      841 aagcggcgtc gctggagacg ttgcaggcgc tgccctggct gcccgacccg gtggcccggg
      901 cgctccactc caagctccac tccgcctgac ccttcctcgt gcaaaaacag tcgcctcacg
      961 accctttttg cacacggaac cgcttgacat gagatagcac gggcgggttg tatgcacacg
     1021 tatgcggggt gcgagggtct tcctcgtcgc cgctctgctg ctgggggcgt gcaacggggg
     1081 gagtggaagc ggttccgaca cgacgctgcc gccgcgcgcc tccacgaccg tgatcgacta
     1141 ctcggtgcct ccggtgatcg atgtcgccta tgtggagcgg gtgatggagg cgctcgaccg
     1201 ggtctacggc gacgccatcc gcacactggc ccgcgagcgc aagatcaccc aagagttcct
     1261 cgaacgcctc gccgcgatct acggcgaagg ccagttcgat cgcactcaag cgatctgggt
     1321 gcgtgatgtc gcttcggggc tgtcaggcct tgccccgacg cccggcgacc cccgcaccgt
     1381 gattcaggag gtgatgcgcg gcgagccctc gtgcgtggtg ttgcgtgtcg accgcgactt
     1441 ctcggcgacg cacgctggcc ctgacgcggc gtcgcctcag gagttcgtcg gcctcattcc
     1501 aaagccgctt gcccgtgatc cgaagt
//
LOCUS       DATADN010000185         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_172373, whole genome shotgun sequence.
ACCESSION   DATADN010000185 DATADN010000000
VERSION     DATADN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172373"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1265
                     /locus_tag="VHH53_02440"
     CDS             108..1265
                     /locus_tag="VHH53_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2289062.1"
                     /translation="MVAEVADWAASLEEVHPRIAGAFARSEPRARVLAYLRGLLGQSE
                     RKNGWTLAEAAGEVSPDGMQRLLRTADWNADAVRDELRGYVVERLGPGGVLIVDETGF
                     IKKGSRSAGVARQYTGTTGKIDNCQIGVFLGYATPAGRALIDRELYLPKAWTDDRERA
                     RAAGIGDDVGFATKPELARRMLTRALDAGVPAGWLTADEIYGQDKRLRVWCEQHGLPY
                     VLATRSNDTVATVDWRQRRIRALIAELPESAWQRCSAGAGAHGLRLYDWARIELLPGF
                     DPGWARWVLARRSIPQIPGENPELAFYVCAGPAGTTLEQLIAVAGGRWRIEECFQAAK
                     NEAGLASYQVRDYTAWYRHITLAMLAHAYLAATRATAEKGAPQPEPSSSSR"
     gene            1268..1417
                     /locus_tag="VHH53_02445"
     CDS             1268..1417
                     /locus_tag="VHH53_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289063.1"
                     /translation="MPELRRLLNTLIRTPRPDLDHTADWSWWRRRRQAQARAAHYRSR
                     GQAPP"
BASE COUNT          226 a          536 c          550 g          217 t
ORIGIN      
        1 cctgctttga ggaatgcagc attagtactg caacggcact tgagggtgtg tcgctgcggg
       61 gatcggctga ggactggtag cagcgtgggc agatgcgctg cgatggcctg gtggccgagg
      121 tggctgactg ggcggcgagc ctggaggagg tgcacccgcg gatcgccggt gcgttcgccc
      181 ggtccgagcc gcgggcgcgg gtactggcct atctgcgtgg gctgctgggc cagtccgagc
      241 gcaagaacgg gtggacgctg gccgaggccg ccggcgaggt gtccccggac gggatgcagc
      301 ggctgctgcg gaccgcggac tggaacgccg acgcggttcg cgacgagctt cgtggctacg
      361 tggtggagcg gcttggcccg ggcggggtgc tcatcgtcga tgagaccggg ttcatcaaga
      421 agggcagccg gtcggccggg gtggcccggc agtacaccgg caccaccggc aagatcgaca
      481 actgccagat cggggtcttc ctgggctacg ccactcccgc cggacgggcg ctgatcgacc
      541 gggagctcta cctgccgaag gcctggaccg acgaccgcga gcgggcccgc gccgccggta
      601 tcggcgacga cgtcggcttc gctaccaagc ccgagctggc ccgccgcatg ctcacccgcg
      661 ccctggacgc cggggtgccg gccggctggc tgaccgccga tgagatctat gggcaggaca
      721 agcggctgcg ggtgtggtgt gagcagcacg gattgcccta tgtgctggcc acccgcagca
      781 acgacacggt ggccaccgtg gactggcggc agcgtcggat ccgtgcgctg atcgctgagc
      841 tgcccgagtc ggcctggcag cggtgctcgg ccggcgcggg cgcgcacggc ctgcgcctct
      901 acgactgggc ccggatcgag ctgctgcccg ggttcgatcc cggctgggcg cgctgggtgc
      961 tggcccgccg cagcatcccg cagatccccg gtgagaatcc cgagctggcc ttctacgtct
     1021 gcgccggacc ggccgggacc accctggagc agctcatcgc ggtggccggc ggtcgctggc
     1081 gcatcgagga gtgcttccag gcggccaaga acgaggccgg actggcctcc tatcaggtgc
     1141 gcgactacac cgcctggtac cgacacatca ccctggcgat gctcgcccac gcctatctgg
     1201 ccgctacccg cgccaccgcg gaaaaggggg ctccgcagcc ggaaccgagc agctcatcgc
     1261 gctgaccgtg cccgaactgc ggcgcctgct caacaccctc atccgcactc cgcggcccga
     1321 cctcgaccac accgccgact ggtcgtggtg gcgccgacga cgccaggccc aagcccgcgc
     1381 cgcccactac cgcagccgag gacaagcccc gccataactg ccgttgcagt actgagtgcc
     1441 gccataggaa cgaacgccac cgcctcgtcc tcagcaggct tgccgccgcc tcgtgggttg
     1501 acgtcggctg cctcccccag agggacaac
//
LOCUS       DATADN010000186         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1223559, whole genome shotgun sequence.
ACCESSION   DATADN010000186 DATADN010000000
VERSION     DATADN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223559"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VHH53_02450"
     CDS             <1..277
                     /locus_tag="VHH53_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289064.1"
                     /translation="ENPSQPGAPANPSQPGAPANPEESGPATLGDLAKKLQSLNPNLV
                     PKADDSDALLEIIVSSASCREDRKKDEKPQDEAPKPKPVEADLPVTH"
     gene            420..908
                     /gene="moaC"
                     /locus_tag="VHH53_02455"
     CDS             420..908
                     /gene="moaC"
                     /locus_tag="VHH53_02455"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX2289065.1"
                     /translation="MSSQQRLTHVDDRGAARMVDISNKQSSTREAVASGILRTTEEVV
                     RLLRAEQLPKGDALATARIAGIMGAKRTPDLIPLCHPIALSGVTVDLHTSGSEVRITA
                     TVRTTDRTGVEMEALTAVAVAGLALHDMVKAVDPAAVLDAVQVERKEGGKTGLWTRQE
                     NR"
     gene            905..>1732
                     /locus_tag="VHH53_02460"
     CDS             905..>1732
                     /locus_tag="VHH53_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HEX2289066.1"
                     /translation="MTTTTRRAVVVVASNRAAAGVYPDRTGPVIVDWLSERGYQTPDP
                     VVVPDGSPVRDAVAVAVADAVDVVLTTGGTGISPTDRTPEATAPLLDRSLPGLADAIR
                     SAGLRQVPTAVLSRGLAGVAGRTLVVNLPGSTGGVRDGLDVLDEVLDHAVDQLHGADH
                     VGSGTGEPAGAHHGHAPSHEESVPAASGAVLRAVVTEEPLDVEEHARLVARPDAGAVV
                     SFSGVVRDHDDSRKVRALEYSSHPGAPDVITRVAVQIAATHPQVQALAVSHRIGPLAI
                     "
BASE COUNT          280 a          582 c          595 g          275 t
ORIGIN      
        1 ggagaacccg tcgcaacctg gtgctccggc caacccgtcg caacctggtg ctccggccaa
       61 cccggaagag tcgggccctg cgaccctcgg ggatctagcc aagaagctcc agtcgctcaa
      121 cccaaacctg gtgcccaagg ccgatgacag tgatgcgctg ctagagatca tcgtgtcgtc
      181 tgccagctgc cgggaagacc gcaagaagga cgagaagcct caggacgagg cgccgaagcc
      241 gaagccggtg gaggcagacc tgccggtcac gcactgacct ggtcaacgca ttcccgacag
      301 ggggctggca cgcatcgcgt gctggccccc ttcgtcgttg ctgccgcctt catcggactg
      361 cccagccacc gaagccgcct gcgcctgatg atcgatggat cccgccgtag gatcgactcg
      421 tgagctccca gcaacgcctg acccatgtcg atgatcgtgg tgcggcccgg atggtcgaca
      481 tctcgaacaa gcagtcctcc acacgggagg cggtagcctc ggggatcttg cgcaccaccg
      541 aggaggtggt gcggttgctg cgcgctgagc agctgcccaa gggcgatgcg ctggccaccg
      601 cacgcattgc gggaattatg ggcgccaaac ggacgcctga cctcatcccg ctgtgccatc
      661 ccatcgcgct gtccggggtc accgtggatc tgcatacctc aggctccgag gttcggatca
      721 ctgccacggt ccggaccact gaccgcacgg gtgtggagat ggaggcgctc accgcggtcg
      781 ctgtggccgg gttggcgttg cacgacatgg tcaaggccgt cgatccggcc gcggtgctcg
      841 acgcggtgca agtggagcgc aaggaaggtg gcaagaccgg gctgtggacg cgtcaggaga
      901 accgatgaca acaacgaccc gccgtgccgt ggtcgtggtg gcttccaacc gtgcggctgc
      961 tggcgtgtat cccgaccgca ccggtccggt catcgttgac tggctgtccg agcgtggcta
     1021 ccagaccccg gatcccgtgg tggtgccaga cggatcgccg gtacgtgatg cggtggcggt
     1081 ggcggtggcg gacgcagtcg acgttgtgct gaccaccggc ggtaccggaa tcagtcccac
     1141 tgatcgcacc ccggaggcca cagcgccgct gctggaccgg agcctgcctg gattggccga
     1201 cgcgatccgt tccgctggat tgcgccaggt gcccaccgcg gtgctgtccc gcgggctggc
     1261 cggggtggcc gggcgcaccc tggtggtcaa tctgcccggc tccaccggcg gagtacgcga
     1321 cggcctggat gtcctcgacg aggtgcttga tcacgccgtt gaccaattgc acggcgccga
     1381 ccacgtcggg agtggaaccg gtgaacctgc tggcgcgcat catggccatg ctcccagcca
     1441 tgaggagtcg gtgcccgcag cctcgggcgc ggtcctgcgt gcggtggtca ccgaggagcc
     1501 gctcgacgtt gaggagcacg cccgcttggt cgcccgcccg gacgccggtg cagtggtgag
     1561 cttctccggt gtcgtccgcg accatgacga cagccggaag gtccgcgcgt tggagtactc
     1621 cagccacccc ggcgctccag acgtgatcac ccgggtagcg gtccagatcg cggccaccca
     1681 tccgcaggta caggcgctgg ccgtcagcca ccggatcggc ccgctggcga tc
//
LOCUS       DATADN010000187         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_323700, whole genome shotgun sequence.
ACCESSION   DATADN010000187 DATADN010000000
VERSION     DATADN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323700"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..723)
                     /gene="meaB"
                     /locus_tag="VHH53_02465"
                     /pseudo
     CDS             complement(1..723)
                     /gene="meaB"
                     /locus_tag="VHH53_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166690.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
     gene            complement(720..1919)
                     /locus_tag="VHH53_02470"
     CDS             complement(720..1919)
                     /locus_tag="VHH53_02470"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX2289067.1"
                     /translation="MTVSVIVAGARTPMGRLLGQLKDFSGAQLGGYAIKSALERAGVA
                     PEQVQYVIMGQVLTAGAGQIPARQAAVNAGIGMDVPALTINKVCLSGLNAIAMADQLI
                     RAGEFDIIVAGGQESMTQAPHLLPKSRAGFKYGDVTMIDHMAYDGLHDSFDQVSMGAS
                     TEKHSARYELTRAEQDEFAAQSHQRAGRAVDSGLFAEEITPVTVPQRKGDPVVVSTDE
                     GIRPDTTAEGLSKLRPAFSADGTITAGSSSPITDGAAAVVVMSKTKAAELGLTWLAEI
                     GAHGMVAGPDDSSLHEQPANAVLAACRKEGIEVSDLDIVEINEAFAAVGVVSTRKLGI
                     DPARVNPNGGAIALGHPIGASGARLALHLAMELKRRGGGVGAASLCGGGGQGDALILR
                     VPGNEAA"
     gene            2050..2496
                     /gene="mce"
                     /locus_tag="VHH53_02475"
     CDS             2050..2496
                     /gene="mce"
                     /locus_tag="VHH53_02475"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX2289068.1"
                     /translation="MKQLQPFVTAIDHVGIAVPDFAAAVEFHKVTFGLELVHEEVNEE
                     QGVHEGMLRVPGDPGTGAAVQLLAPLGPTSTIAKFLDRSGPGLQQLAYRVTDIEAATQ
                     ALRANGIRLLYETARRGTADSKINFVHPKDAGGVLVELVEPAAAER"
     gene            2801..>3309
                     /locus_tag="VHH53_02480"
     CDS             2801..>3309
                     /locus_tag="VHH53_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2289069.1"
                     /translation="MGALPVPESYRGITVHADEADMFAGMPSRDKDPRRSLHLDEVPV
                     PELGPGEALVAVMASAINYNTVWTSIFEPLPTFGFLKRYGRTSPLASRHDLPYHVVGS
                     DLAGVVLRTGAGVNTWRPGDEVVAHCLSVELEHPDGHNDTMLDPEQRIWGFETNFGGL
                     AELALVKSN"
BASE COUNT          551 a         1155 c         1070 g          533 t
ORIGIN      
        1 cccggccttg gttgccaccg tggtgacgat cggtggccgc cagctcgggc cttcgcgttc
       61 ccggcgaccc agcgagatca tgtgtttgag gtcccggacg gtctggtcgg cgccgtcccg
      121 gtcagccttg ttcaccacga agacatcggc gatctcaagg attcaggcct tggccgcctg
      181 gattccgtca cccatgccag gcgccagcag taccacggtg gtgtcggcca acgagacgac
      241 ctcgatctcg gactggccga ctccaaccgt ctccaccagc accacgtcac agcccgcggc
      301 gtcgagcacc cgcagcgcct gcggggtggc ccaggcggtg ccccccaggt gtccacgggt
      361 ggccatcgag cggatgaata caccgtcgtc gagcgcgtga tcctgcatcc gcacccggtc
      421 acctagcaag gccccgccgg agaacggtga ggacgggtcg acggcgagca cgccgacctt
      481 cttgccggcc actcgcagcg cagtgacaag caggttgacc gacgtcgact tgcccactcc
      541 gggcgcgccg gtgataccga caatctgggc acggccggtg tagggggcca gcgcagctgc
      601 cgcgtcgcgt agctccggag cgccgtcttc gatcagcgag atcaaccggg cgaccgcccg
      661 ggcctggcct tgccgtgcgt ggaggaccag gtcctcgata tcgaaccggc gacgagaact
      721 cacgctgctt cgttgccggg gacccgcagg atcagcgcgt caccctggcc accaccgccg
      781 cacagtgagg ccgcgcccac gccgccaccg cggcgcttga gctccatcgc cagatgcagg
      841 gccagccgcg ccccggacgc accgatcggg tgacccagcg cgatcgcgcc gccgttggga
      901 ttgacccgcg ccgggtcgat gcccagcttg cgggtggaca ccacacccac ggcggcgaac
      961 gcctcgttga tctcaacgat gtcgaggtcg ctcacctcga tgccctcctt gcggcaggcc
     1021 gccaataccg cattggcggg ctgttcatgc aggctggagt cgtcaggacc ggccaccatg
     1081 ccgtgtgcgc caatctcggc cagccaggtc aggcccagct cagccgcctt ggttttgctc
     1141 atcaccacga cggcagcggc cccatcggtg atcggagacg acgatccagc ggtaatggtg
     1201 ccgtcagcgg agaacgcggg ccgcagcttg ctcaacccct cagcagtggt gtccggccgg
     1261 atgccctcat cagtcgacac cacgacggga tcacccttgc gctgcggcac ggtgaccgga
     1321 gtgatctcct cggcaaacag cccactgtcc actgcacgcc cggcccgctg atgtgactgc
     1381 gcggcgaact cgtcctgctc ggcccgggtc agctcgtagc gcgcactgtg cttctcggtg
     1441 gaggcaccca tcgagacctg gtcgaacgag tcgtgcaggc cgtcataggc catgtggtcg
     1501 atcatggtga cgtcgccgta cttgaacccg gcgcgtgact tgggcagcag atgcggcgct
     1561 tgggtcatcg actcctgccc accggccacg atgatgtcga actcgccggc ccgaatcagc
     1621 tgatcggcca tcgcgatcgc attcagcccg gacaggcaca ccttgttgat ggtcagggcc
     1681 ggcacgtcca tcccgatgcc ggcgttgacc gccgcctggc gcgccgggat ctgtcccgcc
     1741 cccgccgtca atacctgtcc catgatcacg tactgcacct gctcaggcgc cacaccggcg
     1801 cgttcgagcg cgctcttgat cgcgtaccca ccgagctggg cacccgagaa gtccttgagt
     1861 tgaccgagca gccgacccat cggggtgcgg gctccggcca cgatgacgga aacggtcatg
     1921 gtgctacctc cggcgggacg acgcgggcga ccagggcgat acgggggttg tgttcgacct
     1981 taccgggcgc gatgaccacc gcacgttggc gacgacgagc acaccccaac tggccctacg
     2041 ctgcgaacca tgaaacaact ccagcccttc gtcacggcga tcgaccacgt gggcatcgcg
     2101 gtgcccgatt tcgctgcggc cgtcgagttc cacaaggtca cgttcggatt ggagctggtt
     2161 cacgaggagg tcaacgagga gcagggggtg cacgaaggca tgctgcgcgt tcccggcgac
     2221 ccgggcaccg gtgccgcggt gcagctgctg gcccccctgg gcccgacgtc caccatcgcc
     2281 aagttcctgg accgcagcgg tcctgggctg cagcagctgg cctaccgggt caccgacatc
     2341 gaagcggcca cgcaggcgct gcgcgccaat ggcatacgac tgctgtacga gacggctcgt
     2401 cgcggcaccg ccgattcaaa gatcaacttt gttcatccca aggacgcggg tggcgtgctg
     2461 gtcgagctgg tggaaccggc agcagccgag cgttaacccc ccgtgcttta gtgatcccgt
     2521 gttcctacct aggcgcgccg accggcagca ccgtcgtcga acctggtgat gtcctcgggg
     2581 tctacggcac ggcgatctgc tccgggagct cggcggcagg acttccggcc cggccggtga
     2641 cctggcccat gatgctgcgg tcgcccgcca gtgcggcatc gcttgatata gtgatcgcta
     2701 gcctcagcct agctagctat cccgagcgga aagcggccag taggaaggga cgaccgtgca
     2761 aaagattctc gatgccattc tcaacggcga catcgacacc atgggcgcgc tgccggtgcc
     2821 ggaaagctac cggggaatca cggtccacgc tgacgaggcc gacatgttcg ccgggatgcc
     2881 cagccgggac aaggacccac gccggtccct gcacctggac gaggtaccgg tgcccgaact
     2941 tggtccgggc gaggccctgg tcgcggtgat ggccagcgcc atcaactaca acaccgtgtg
     3001 gacgtcgatc ttcgagccgc tgcccacgtt cgggtttctc aagcgttacg gccgcacctc
     3061 cccgttggct tcccggcacg acctgcctta ccacgtggtc gggtcggacc tggccggcgt
     3121 ggtgctgcgt accggagccg gggtgaacac ctggagaccc ggcgacgagg tggtcgccca
     3181 ctgcctgtct gtcgagctgg agcatcccga cgggcacaat gacacgatgc tcgaccccga
     3241 acagcgcatc tgggggttcg agacgaactt cggcggtctc gccgagctgg cgttggtcaa
     3301 gtccaacca
//
LOCUS       DATADN010000188         4080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1223613, whole genome shotgun sequence.
ACCESSION   DATADN010000188 DATADN010000000
VERSION     DATADN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223613"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VHH53_02485"
     CDS             complement(<1..948)
                     /locus_tag="VHH53_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX2289070.1"
                     /translation="MRRRQHLAHLDANLCEFEKEQAAPTFKHTFGYHPMLAFLDNTGE
                     FLAAVLRGGNAGSNTAADHITVLDAALAQIPDAHRHGTPIFIRADTAGATKEFLAHFR
                     GLREKDMSCSFSVGWAITERERAAISTLPNTVWAEAIDTHGRHRDGAGLAELTCVLPA
                     RALAGYPAGTRVIVRRERPHPGAQLDAFEERDGWRYTAFVTDTPFGQLAALDARHRAH
                     ARVEDRIRTAKDTGLDHFPSRAFAINAAWLTIVMIAVDLIDWAQHLLLHGDLAKAEPK
                     TLRYRLLHVAACLVRGQRRTRIRIDHRWPWARQLADAFNR"
     gene            complement(957..1799)
                     /locus_tag="VHH53_02490"
     CDS             complement(957..1799)
                     /locus_tag="VHH53_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289071.1"
                     /translation="MEFNQLQLRDRERLRRIGARPISDITIETKLRILRDLARHLTGS
                     RRLTSWTEVTTADLESFLAQTPQARHQQTYLLRAFFGWAKHRRLLLVDPARVLRLGAQ
                     PGFTGTLLEPSAQRTLLQRWTDGQTHPHERLGGLLALLHAASNLETRTLTLDDIDHQR
                     HTVALGKRPFPTPLDPTTWTALQACLTHRLTLSTLNPHLIVTAVTRTRTTPADSTYLT
                     RQLARAATTPSTCRQTRIAQLVTDLDPKLAATALGMQDSGLVRYLADNVDHDRLQRSV
                     PGHS"
     gene            complement(2424..2729)
                     /locus_tag="VHH53_02495"
     CDS             complement(2424..2729)
                     /locus_tag="VHH53_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2289072.1"
                     /translation="MQIRWRLRMTAAQREVWTGAQLRRLLAERAGLELSSASVSALFT
                     RQPSQVKLDTLAALCTALQCTPNDLFEVDTTPVAVTKPARKTEPEPKAARGRSMPPL"
     gene            complement(2719..3567)
                     /locus_tag="VHH53_02500"
     CDS             complement(2719..3567)
                     /locus_tag="VHH53_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2289073.1"
                     /translation="MIAGLVAQGLAASTRRSYVQSFKGFHAFLVARKAAEIEAAFGVR
                     LIDPVDEFNAARHVAVDSPAVKAPPDPERMEQFFDFLKTRIASARKYVAAGRDYALFR
                     TLYLAGLRAEEASALDRSDVHFGRGPFGKLHVRFGKGAKTSGPRPRWVPLLDGLDLIL
                     RWYLQDIHPRLGDGSALFCDEGGGRIHRGTIRNRLAHLLDCEAAAAGEAALARFSPHG
                     LRHACATRNYERGVDLVAIQQMLGHWHVGTTMRYVSPSATFIEDAYRRAVSGALASLE
                     GQADAD"
     gene            complement(3564..3800)
                     /locus_tag="VHH53_02505"
     CDS             complement(3564..3800)
                     /locus_tag="VHH53_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289074.1"
                     /translation="MARDQFRVLQGGSSAPSAVAALVDPVAFQRMCLEAYEASQVARG
                     FSPVTMDNWNATLERFLAACAGRRGRSLVRMWIG"
     gene            complement(3900..>4080)
                     /locus_tag="VHH53_02510"
     CDS             complement(3900..>4080)
                     /locus_tag="VHH53_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289075.1"
                     /translation="PRLDTRSSCSAGWIGVVETGMGLGGGAVQDGKVVIDLDATWSPR
                     IHLGLAQVREGQDRQ"
BASE COUNT          660 a         1337 c         1450 g          633 t
ORIGIN      
        1 gcggttgaat gcatcggcga gttggcgcgc ccatggccag cggtggtcga ttctgatccg
       61 ggttcggcgt tgcccgcgga cgagacaggc ggcgacgtgc agcagccggt agcgcagcgt
      121 cttgggttcg gccttggcca gatcgccgtg caggaggagg tgctgggccc agtcgatcag
      181 gtcgacagcg atcatgacga tggtgagcca ggcggcgttg atcgcgaatg ctcgactggg
      241 gaagtggtcc agtccggtgt ccttggcggt gcggatgcgg tcttcgacac gggcgtgggc
      301 gcggtggcgg gcgtcaagcg cggcgagttg cccgaacggg gtgtcggtga caaaggcggt
      361 gtagcgccaa ccatcgcgct cttcgaacgc gtcgagctgg gcaccggggt gcgggcgttc
      421 gcggcgcacg atgactcggg tcccggcggg gtagccggcc aacgcacggg cgggtaggac
      481 gcaagtgagc tcggccaacc cggcaccgtc gcggtgtcgg ccgtgggtat cgatcgcctc
      541 ggcccacacc gtgttcggca gggtgctgat cgcggcgcgt tcccgctcgg tgatggccca
      601 gccgacggag aaactgcagc tcatatcctt ctcgcgcagc ccacggaaat gggcgaggaa
      661 ctccttggtg gccccggcgg tgtcggcccg gatgaagatg ggggtgccgt ggcggtgggc
      721 gtcggggatc tgcgcgagcg ctgcgtcgag cacggtgatg tggtcagcgg cggtgttgct
      781 gccggcgttg ccgccgcgca gcactgcggc cagaaactct ccggtgttgt ccaaaaaggc
      841 cagcatgggg tggtagccga aggtgtgttt gaacgtcggc gccgcttgct ccttctcgaa
      901 ctcgcacagg ttcgcgtcaa ggtgtgcaag gtgctgcctg cggcgcacgg gttggctcag
      961 gagtggccgg gaacgctgcg ttggagccgg tcgtgatcga cgttgtcggc gaggtagcgc
     1021 accagtccgg agtcctgcat gcccaacgcc gttgccgcca gcttggggtc aaggtcggtg
     1081 acgagttgcg cgatccgggt ctgtcggcag gtcgacggcg tggtcgcggc tcgggcgagc
     1141 tggcgggtga ggtaggtgct gtcggccggg gtggtccggg ttcgggtgac ggcggtgacg
     1201 atcagatgtg ggttgagggt gctcagcgtc agccggtgcg tgaggcaggc ttgcagcgcc
     1261 gtccatgtcg tcgggtcaag tggagtgggg aacggtcgct tcccgagcgc gacggtatgc
     1321 cgctggtggt cgatgtcgtc cagtgtgagg gtgcgggtct ccaggtttga tgcggcgtgc
     1381 agcagggcga gcagtccgcc gagccgttca tgtgggtggg tttggccgtc ggtccagcgc
     1441 tgcaggaggg tgcgttgcgc agagggctcg aggagtgtcc cggtgaaccc gggttgtgca
     1501 cccagccgca gcacacgagc cgggtcgaca agcagcagcc gacggtgttt ggcccagccg
     1561 aagaaggctc gcagtagata ggtctgctgg tggcgggcct gcggggtctg ggcgaggaag
     1621 gactccaggt cggcggtggt gacttcagtc cagctggtca gtcggcggct gccggtgagg
     1681 tggcgggcga gatcgcgcag aattcgcagc ttcgtttcga tagtgatgtc gctgatcggg
     1741 cgtgcgccga tccggcggag gcgttctcgg tcgcgcagct gcagctggtt gaactccacg
     1801 gtcgccggcc gcagcgctgc cgggacagtt ccagcaagtg ctggcgacgg gtggcggcgc
     1861 ggcgttgggc gtcgtcggcg ggaaacgcca agccgtggct ggtgaagaag gcggtcaatg
     1921 cccgggacag cggccgatcg ggaccatcgg gttcgcgact gtgggacagc agctgctgcg
     1981 gcgcgaatcc cggattggcg tcgaggagcc ggccgagctg gcgcagcaga tccaccgcac
     2041 caccgggata gttggaggcc gcgacgaacg cggtgaaccg ttcccaccag tccggcctcg
     2101 ccttcagccg ctcgccgagt ttggcgccgt agcggaaagg ccgatccggg tcggccagct
     2161 gacagcggtt gcacaacccg tgcccggtgt tgcggcggcg ctgtccgcag cgcacgcagg
     2221 tggtgagctt gggtaaccgg cgcggcgcgt cggcccgagc gcagccgcgc agatccccga
     2281 cggacgcaga tgccccagca ggccgcaacg caggcacggc cgcttgaggg cctcgcgagc
     2341 ggcgcggcgg tgacagacgt ggcagaggtc ccgatgagcc ggtctgaccg gcgccgcgca
     2401 gtcccgacaa gcccgtgtcg gcgtcacaac ggcggcatcg atcgcccgcg ggccgctttc
     2461 ggctcgggtt cggtctttcg tgccggcttg gtcaccgcga ccggtgtggt gtccacttcg
     2521 aacagatcgt tgggggtgca ctgcagcgcg gtgcacaacg cagccagggt gtccagcttg
     2581 acctgagagg gctgcctggt gaacagcgcc gacaccgacg ccgatgacaa ctccaaccca
     2641 gcccgctcgg cgagcagccg gcgcagttgg gcaccggtcc agacctcgcg ctgcgcggcg
     2701 gtcatccgca gccgccacct aatctgcatc ggcctgtccc tccaagctgg ccagcgcgcc
     2761 ggagacggca cgccggtagg catcctcgat gaacgtcgcc gacggcgaca cgtaccgcat
     2821 cgtggttccg acatgccagt gcccgagcat ctgctggatg gcgaccaggt ccacgccacg
     2881 ctcgtagtta cgggtcgcgc aggcatgccg caggccgtgc gggctgaacc gggccaaggc
     2941 cgcctcgccg gccgcggcgg cttcgcagtc cagcagatgg gccagccggt tgcggatggt
     3001 gccgcgatgg atccgaccgc cgccttcgtc gcagaacagc gccgacccgt cacccaaccg
     3061 cgggtggatg tcctgcagat accagcgcag gatcaaatcc aggccatcca gcaacggcac
     3121 ccaccgcggc cgcggacccg acgtcttcgc gcccttgccg aaccgcacgt gcagcttgcc
     3181 gaacgggccc cgaccgaaat ggacatccga acggtccaac gcggaggcct cctcggcgcg
     3241 caacccagcc agatacaagg tgcggaacaa ggcatagtcc cgcccggccg ccacgtactt
     3301 acgggccgac gcgatccggg tcttcaggaa gtcgaagaac tgctccatac gctccggatc
     3361 cggcggcgcc ttgaccgccg gggaatcaac ggccacgtgc cgggcggcgt tgaactcatc
     3421 caccgggtcg atcagccgca ccccgaacgc ggcctcgatc tcggctgcct tgcgggccac
     3481 cagaaaggcg tgaaacccct tgaacgactg cacataagac cgccgagtcg aagccgccaa
     3541 cccctgcgcc accaaccccg cgatcacccg atccacatcc tcacgagtga tctcccacgc
     3601 cggccggcgc aggcggcgag aaaccgctcc agcgtggcat tccagttgtc catcgtcacc
     3661 gggctgaacc cgcgggccac ctgagacgcc tcgtaagcct ccaaacacat cctctggaac
     3721 gccaccggat caaccagcgc cgccaccgcg gacggagcgc tcgacccgcc ctgcagcacc
     3781 cgaaactgat ccctggccac cgcccacctt ccccacacca tgcaagtgaa ggcggcacct
     3841 tacagaccag gcctcaacct cacgatcatc gaccctcccc acgagccgat cagcccagct
     3901 cactgccgat cttgaccctc acgaacctgt gccaacccca ggtgaatgcg cggtgaccag
     3961 gtggcgtcca gatcgatcac cactttccca tcctggaccg cgcccccgcc cagccccata
     4021 cccgtctcga caactccgat ccaaccggct gagcaggaac ttcgcgtatc taacctcggc
//
LOCUS       DATADN010000189         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_623023, whole genome shotgun sequence.
ACCESSION   DATADN010000189 DATADN010000000
VERSION     DATADN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623023"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /gene="arfB"
                     /locus_tag="VHH53_02515"
     CDS             <1..336
                     /gene="arfB"
                     /locus_tag="VHH53_02515"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX2289076.1"
                     /translation="QGVNTTDSRVELIFDVAASTALTPAQRDRIRQRLSGRLVNGVLT
                     VVAAEHRSQLRNREAARSRLAAILRQAVVPDPPTRRPTRPSRAARERRLADKRRRTLT
                     KRLRRPAEE"
     gene            422..943
                     /locus_tag="VHH53_02520"
     CDS             422..943
                     /locus_tag="VHH53_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2289077.1"
                     /translation="MTSRQPPNNGRHAKPDAPSGVRSLPLSPDPPRHHADASLGELVR
                     EAATHFSVLFRSEVELAKAEVTAEVRKGVKGSIFFVIALTILIFSLFFLFIALAQVLA
                     IWLPQWAAFSVIFGLMVVAAAGFALVGWRRVRSVRKPERTLSTVRDTAAVLAHPRSRQ
                     HAIDQHSADQPRE"
     gene            940..1863
                     /locus_tag="VHH53_02525"
     CDS             940..1863
                     /locus_tag="VHH53_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2289078.1"
                     /translation="MSRPPDSSSVRIPGHWTHREISANGIRLHVAEVGTGPLVLLLHG
                     FPEFWWSWRHQLPALAAAGRRAVAVDLRGYGDSDKPPRGYDLWTLSGDVAGLVRALGE
                     RQADLVGHDWGGILGWCTAALHPRLVRSVTVLSAPHPLAMREALLRDPAQRCASRYLA
                     GFQVPWLPERNLIRDRAALVERILRRGASSQWRASSEFTIAARRYRDAMLIQGTAHCA
                     LEYYRWVGRSQFRFDGHRLSRAVNHPVRVPVLHLHGADDPYVLEPTARRSGQWAGSNY
                     RYEVLTGVGHFVQQEAPEHTATLIAEFLRDR"
BASE COUNT          273 a          657 c          640 g          322 t
ORIGIN      
        1 cagggtgtca acacgaccga ctcgcgggtc gaattgatct tcgatgtggc tgccagcacc
       61 gcgttgactc cagcgcagcg ggatcggata cgccagcggc tgtccgggcg gctggtcaat
      121 ggggtgctca ccgtcgtggc cgccgagcac cgctctcagc tacgcaaccg ggaggctgcg
      181 cgaagccggc tcgccgcgat actgcgccag gcggtcgttc cggatccgcc gacgcgccgc
      241 ccaacccgcc cttcccgggc cgccagggag cgccgcctgg ccgacaagcg tcgtcggacg
      301 ctcaccaaac ggctgcgccg accagctgag gagtgagtgg gcacccgcct caggggcggc
      361 cacgctcagt tcggtacggc cacagtcagt ggtcaagacg ggtcgtggca ccatgtcgcc
      421 cgtgactagc aggcagcctc ccaacaacgg ccggcacgcc aaaccggatg caccgtccgg
      481 agtgcggtcc ctgccgctat cgccggaccc tccccgccat catgccgacg catccctggg
      541 cgagctggtg cgcgaggccg cgacgcactt ctccgtcttg ttccgctcgg aagtggaact
      601 ggccaaggcg gaggtgacag ctgaggtccg caagggtgtc aagggcagca tcttcttcgt
      661 catcgccctg acgatcctga ttttcagcct gttcttcctg ttcatcgcgc tggcccaagt
      721 gctggccatc tggctgccgc agtgggccgc gttcagcgtc atcttcggcc tgatggtggt
      781 cgccgccgcg ggcttcgcgc tggtgggatg gcgccgggta cgcagcgtcc ggaagccgga
      841 acgcacgctc agcaccgtcc gcgataccgc ggcggtgctc gctcacccac gttcccgcca
      901 gcatgccatc gaccagcaca gcgccgatca accccgggag tgagccgtcc accggattcc
      961 agctcggtcc ggattcctgg gcactggacg caccgggaga tatccgccaa cgggatccgg
     1021 ttgcatgtgg ccgaggtcgg gaccggcccg ctggtgctgt tgctgcacgg ctttccggag
     1081 ttctggtggt cctggcgtca ccaactcccg gcgctggccg cagcgggtcg ccgtgccgtt
     1141 gccgtggatc ttcgcggtta cggtgactcc gacaaaccac cccgcggcta tgacctgtgg
     1201 acgctgtccg gtgacgtggc ggggctggtc cgggcgctcg gtgaacgcca agccgatctg
     1261 gtagggcatg actggggcgg gatcctcggt tggtgcacgg ccgcgttgca ccccaggttg
     1321 gtgcgttcgg tcacggtgct gtcggccccg catccactcg cgatgcgaga ggcactgctg
     1381 cgcgatccgg cacaacgctg cgcttcgcgc tacctggccg gcttccaagt cccgtggctg
     1441 cccgagcgca atctcattcg cgaccgtgcg gcgctggtgg agcggatcct gcgccgcgga
     1501 gcgagctcgc agtggcgcgc gtcgagcgag ttcaccatcg cggcccggcg ctatcgcgat
     1561 gccatgctga tccagggcac tgcgcactgc gcgctggagt actaccggtg ggtcggccgc
     1621 tcccagttcc ggttcgacgg ccaccggctg tcccgtgcgg tgaaccaccc ggtccgggtt
     1681 ccggtgctgc atctgcacgg cgctgacgac ccgtacgtgc tggagccaac ggcgcggcgc
     1741 tcaggccagt gggcaggcag caactatcgc tatgaggtac tcaccggcgt cggtcatttc
     1801 gtgcagcagg aagcacccga acacaccgcc acgctcattg ccgaattcct gcgggatcgg
     1861 tgagctcacg cggcgtctgc gccgcgtgag ct
//
LOCUS       DATADN010000190         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_323981, whole genome shotgun sequence.
ACCESSION   DATADN010000190 DATADN010000000
VERSION     DATADN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323981"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VHH53_02530"
     CDS             complement(<1..1493)
                     /locus_tag="VHH53_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2289079.1"
                     /translation="MKSALLHDAHGDRLSDPGAVERFVAEARVWVDLEPHPNICACHY
                     VQVLRGAPRIFMEYVEGGSVADELRAGRMNTLAEMLGVGIQMAWGLAAIHDHNVVHQD
                     VKPGNVLLAADGRVKITDLGLARARAATTSAFGVADAELVTRQGMTPPYASPEQWTGR
                     RVGSATDVWSWAVSILELFTGQVTWSFGPAARDVLAHYLDQQPSVGRAGMPADVAALL
                     GECLADDPGDRPTMDTVADRLVALYETHIGVYPHQAGGAAQRLAGEWNNKALSLLDLG
                     DPVAAQECWKQALRVDPHHPGAMFNHGLTRWRSARITDQRLITDLENLLADLDERRLI
                     RRPADFGETGGNVLHLLALVHLERGDAHSAVELLQEAVEFAADDPVVLAALTTATASA
                     HRLREPRTLTGHTDWLNSVAVTPDGRRAVTGSSDHTARWWDLTTGRALHTLAGHTATV
                     TSVAVTPDGGRVLTGSRDHDARWWDVATGQVLRTLAGDGPISAVAVTP"
BASE COUNT          244 a          537 c          516 g          238 t
ORIGIN      
        1 ggcgtcaccg ccaccgccga gatcgggcca tcgccggcga gggtgcgcag gacttggccg
       61 gtggcgacgt cccaccatcg cgcgtcgtga tcgcgactgc cggtgagaac gcgcccgccg
      121 tcaggggtca ccgccaccga ggtgaccgtg gcggtgtggc cggcgagggt gtggagggca
      181 cggccggtgg tgaggtccca ccatcgcgcc gtgtgatcac tgctgccggt gacagcgcgc
      241 cgaccgtcag gcgtcaccgc caccgagttg agccagtcgg tgtggcctgt gagggtgcgg
      301 ggttcgcgaa ggcggtgcgc gctcgcggtg gcggtggtca gggcggccag gacgacgggg
      361 tcgtctgcgg cgaactcgac ggcctcctgc agcagttcga ccgcgctgtg ggcgtcgccg
      421 cgttcgaggt ggaccagcgc gagaagatgg agcacgttcc cgcccgtctc accgaagtca
      481 gcgggacgtc gaatcaagcg acgctcgtcc agatcggcga gcaaattctc cagatcggtg
      541 atgaggcgct ggtcggtgat ccgtgcgctc cgccaccgtg tcaggccatg gttgaacatc
      601 gccccggggt ggtgcgggtc gacgcgcagc gcctgcttcc agcattcctg tgccgcgacg
      661 ggatcgccga ggtcgagcag tgagagcgct ttgttgttcc actcaccggc gagccgctga
      721 gccgcgccgc cggcctggtg cggatacacc ccgatgtgcg tctcgtagag cgcgaccagc
      781 cgatcagcga ccgtgtccat cgtgggacga tcacccgggt cgtcggccag gcattccccc
      841 agtagcgcgg ccacgtccgc gggcatgccc gcccgaccca ccgacggctg ctgatcgagg
      901 taatgggcga gtacgtccct ggcggccggg ccgaacgacc aggtcacctg cccggtgaac
      961 aactccagaa tcgacaccgc ccacgaccac acatcggtgg ccgagccgac ccgccgcccg
     1021 gtccactgct ccggcgaagc gtacggcggg gtcatcccct gccgggtgac cagctccgca
     1081 tcggccactc cgaaagctga ggtcgtggcg gcccgcgctc tcgccagccc caggtccgtg
     1141 atcttgactc tgccgtcggc ggccagcagc acgttgcccg gtttcacgtc ctggtgcacg
     1201 acgttgtggt catggatggc cgccaggccc cacgccatct gaatcccgac cccgagcatc
     1261 tcggcaaggg tgttcatccg gccggcgcgc aattcatccg cgacgctgcc gccctcgaca
     1321 tactccatga agatccgcgg tgcgccgcgc aatacctgca cgtaatgaca tgcacatatg
     1381 ttcggatgag gttcaagatc tacccacacc cgcgcctcgg caacgaaccg ctccactgct
     1441 cccgggtcag acagccgatc accatgcgcg tcgtgcagaa gcgcgctctt caccgccagg
     1501 tcgactcccc acccgaggtg atgaacgaga tcgac
//
LOCUS       DATADN010000191         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1077831, whole genome shotgun sequence.
ACCESSION   DATADN010000191 DATADN010000000
VERSION     DATADN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077831"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VHH53_02535"
     CDS             <1..1102
                     /locus_tag="VHH53_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX2289080.1"
                     /translation="DLPALIEQLHGADLAVLGYLSPFLVPGRPGFDEARERGYLVPTP
                     AAPVFTEPWAEDDHRAYLDFTRPDAVAWWQDRVRHAMGTVGFDGAMQDYGEGAPVDGI
                     YASGQPGTMVHNAYPVRYAQAARQAAQAVKPGQTVLFARAGYSGSQASVTGRFTGDQH
                     RSWDRHTGIGSVLPAMLSGSISGWPYWGPDIAGFFDGDGSRDVELWTRWTQLGALSPV
                     MRDMLGAQADPIGVFSNDGTLATFAGYARLHQALMPYLHELAEQASRTGLPLIRPLWL
                     AEPADPVAWTVEDAYLLGEDVLVAPVVTEGARSRPVYLPAGQWHDYWTGEVITGARWI
                     SARAPVQQIPLYVRTGANLALPLPATLGLPAG"
     gene            1208..2077
                     /locus_tag="VHH53_02540"
     CDS             1208..2077
                     /locus_tag="VHH53_02540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289081.1"
                     /translation="MTGKVTVIVATRDRRDELTHTLCRLSELTPPPPIVVVDNGSTDG
                     TAHAVRTQFPEVTLLALRHNHGAPGRNLGVQASRTPYIAFSDDDSWWAHGALQRAAQI
                     LDAHPRLGLLAAHTLVGPQQRPDPIAQMMAESPLPRPPDAAGPAVLGFLACSAVLRRE
                     AFLSVGGFSKVIFFPGEEQLLCYDLAAAGWDRAYVADVVAHHHPSTHRGNPVRRRALE
                     RRNALLTSVMRRPPRVAAASGAALARAAVRDASARRALRGALVRLPAALRRRAPLPAA
                     VEEQIRMLEEGPQ"
     gene            2074..>2241
                     /locus_tag="VHH53_02545"
     CDS             2074..>2241
                     /locus_tag="VHH53_02545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289082.1"
                     /translation="MTGGRVTVVIITHNRRAELLRTLARMTALPDQAPVVVVDNASTD
                     GTCDAVAELFGQ"
BASE COUNT          308 a          799 c          798 g          336 t
ORIGIN      
        1 cgacctgcca gcgctgatcg agcagctgca cggtgcagat ctggcggtac tcggctacct
       61 cagccccttc ctggtccctg gccggccggg gttcgacgag gcccgcgagc ggggctacct
      121 ggtgcctacc ccggcagcgc cggtgttcac cgaaccctgg gccgaagacg accaccgggc
      181 ctacctggac ttcacccgcc ccgatgccgt cgcgtggtgg caggatcggg tgcgccacgc
      241 catgggcacg gtgggtttcg acggcgccat gcaggactac ggggagggcg ctccggtcga
      301 cgggatctac gccagcgggc agccaggaac gatggtgcac aacgcctacc cggtgcgcta
      361 cgcccaggcg gccaggcagg cggcgcaggc ggtcaagcca gggcagacag tgctgttcgc
      421 ccgcgccggc tacagcggct cccaggccag cgtcaccggc cggttcaccg gtgaccagca
      481 ccgctcctgg gaccggcaca ccggtatcgg cagcgtgctg ccggcgatgc tgtccggctc
      541 gatctccggc tggccgtact gggggcccga catcgccggg ttcttcgacg gcgacggttc
      601 acgcgatgtc gagctgtgga cccgctggac ccagctgggt gcgctgtctc cggtgatgcg
      661 cgacatgctc ggcgcccagg ccgacccgat cggggtgttc agtaacgacg gcaccctcgc
      721 cacgttcgcc gggtacgcgc ggctgcacca ggcgctgatg ccgtacctgc atgagctcgc
      781 cgagcaggcc agccggaccg gtctgccgct gatccggccg ctgtggctgg ccgagccggc
      841 cgacccggtg gcctggacag tggaggatgc ctacttgctc ggggaggacg tgctggtggc
      901 cccggtagtc accgaaggtg cgcggagccg tccggtgtat ctgccggcgg ggcagtggca
      961 tgactactgg accggtgagg tgatcaccgg tgcccgctgg atcagcgccc gcgcgcccgt
     1021 gcagcagatc ccgctgtatg tccgcaccgg cgcgaatctc gcgctgccgc taccggcgac
     1081 gttgggcctg cccgccggat gacgcggcct ccgggaactc gacgtaggcg acgtgtgtcg
     1141 gtgttggtga cgtttcgcgg tgtgaccggc gggtagtcaa cggcgtgcga gtccttgggg
     1201 ggcacgggtg accggcaagg tcacggtgat cgtcgcgacc cgggaccggc gagacgagct
     1261 cacccacacg ctgtgccggc ttagcgagct gaccccgccg ccgcccatcg tcgtcgtgga
     1321 caacggctcg accgatggca ctgcgcatgc ggtgcgcacc cagttccctg aggtgacgct
     1381 gctggcgcta cgccacaacc acggcgcgcc aggccgcaac ttgggcgtac aagccagccg
     1441 cacgccctac atcgccttca gtgacgacga ctcgtggtgg gcgcacggcg ccctgcaacg
     1501 cgccgcacag attcttgatg ctcaccctcg gctgggcctg ctggccgccc acaccctcgt
     1561 cgggcctcag cagcgccccg acccgatagc gcagatgatg gcggaaagcc cgctgccccg
     1621 gccgccagac gctgccggac cggccgtact ggggttcctg gcgtgctcgg cagtgctgcg
     1681 ccgggaggcg tttctgagtg tcggcgggtt cagcaaggtg atctttttcc ctggcgagga
     1741 acaactgctc tgctacgacc tggccgcggc cggctgggac cgcgcctacg tcgcggacgt
     1801 ggttgcccat caccacccct cgacgcatcg gggcaaccca gttcgccgcc gggcactgga
     1861 gcgccgcaac gccttgctca cctccgtgat gcgccgcccg ccgcgggtgg cagccgcctc
     1921 cggcgctgcc cttgcacgcg cggcagtgcg cgacgccagc gcccgccgcg cactgcgtgg
     1981 ggcgctggtc cggctcccgg cggcgctgcg gcggcgggcg ccactaccgg ccgcggtgga
     2041 ggagcagatc cgcatgctcg aggaaggtcc gcagtgaccg gtggccgggt caccgttgtg
     2101 atcattaccc ataaccgtcg ggccgagctg ctgcgcacgc tggcccgcat gaccgcgctg
     2161 ccagaccagg cgcccgtggt ggtggtcgac aacgcgtcga ccgacggcac ctgcgatgcg
     2221 gtagccgagc tcttcggcca g
//
LOCUS       DATADN010000192         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_927537, whole genome shotgun sequence.
ACCESSION   DATADN010000192 DATADN010000000
VERSION     DATADN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927537"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VHH53_02550"
     CDS             complement(<1..467)
                     /locus_tag="VHH53_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX2289083.1"
                     /translation="MTQTAVLTIPAQLKPGDGRFGCGPSKVRPEQLAALATAGASIMG
                     TSHRRNPVKSLVARVRSGLRELFSLPEDYQVVLGNGGTTAFWDAAALGLVRERSLHLS
                     YGEFSAKFVGVTKGAPFLADPVVVTAAPGDAPQPGADPSCDLIGWAHNETSTGV"
     gene            complement(521..1357)
                     /locus_tag="VHH53_02555"
     CDS             complement(521..1357)
                     /locus_tag="VHH53_02555"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2289084.1"
                     /translation="MEHISAGKVRDLYSIGDDILLVASDRISLYDVALPTPIPDKGAL
                     LTQLSVWWFRQLGDVVPNHLISATDVPPEFMRRAIRAKPLQMIPVECIARGYLAGLGL
                     REYDKFGTVSGVALPPGLVSGSRLPEPIFTPTTKYSDTGHDEFITFDDVVRGTGRDTA
                     QRLRDLTLELYTAAAEHAAGNGIIVADTKFEFGWDTDGRLTLGDEALTSDSSRFWPAD
                     TWRPGQQQHSLDKQFVRDWSISTGWNTEPPGPVIPPAIVAETRRRYIEVYERITGASW
                     PA"
     gene            1429..>1810
                     /locus_tag="VHH53_02560"
     CDS             1429..>1810
                     /locus_tag="VHH53_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX2289085.1"
                     /translation="MDAHSTFHDLVGQLDYSMFIITVRAGDEQAGCLVGFCTQSSIDP
                     PRFLVCLSRKNRTYRVAGRTDLVAVHFVPRRAGALAELFGGHSGDDEDKFARCDWQPG
                     PGGHSHRQCRRTQRLRRAGQLPAAG"
BASE COUNT          309 a          588 c          595 g          318 t
ORIGIN      
        1 acgccggttg aggtttcgtt gtgcgcccag ccgatcaggt cgcaagacgg gtcggccccg
       61 ggctgcgggg catccccggg cgcagcggtg accacaaccg ggtcggccag gaagggggcg
      121 cccttggtga ccccgacgaa cttcgctgag aactcgccgt agctcaggtg cagcgagcgc
      181 tcccggacca gacccagggc tgcggcatcc cagaacgccg tggtgccgcc gttgccgagc
      241 acgacttggt aatcctcggg cagcgagaac agctcgcgta accccgagcg aacccgtgcg
      301 accagtgact tcaccgggtt gcgccggtgc gaggtgccca tgatcgaggc acccgcggtg
      361 gccagcgcag ccaactgctc gggtcggact ttcgacggcc cacatccgaa gcggccgtct
      421 ccgggcttga gctgggccgg gatggtcagc acggcggtct gcgtcatgtc gcgggtctcc
      481 atggttgctg gccgggagtc cagtttcgca ggctcgcagg ctacgccggc cacgaggccc
      541 cggtgatgcg ctcgtatacc tcgatgtacc gccgccgtgt ttccgccacg atcgcagggg
      601 gaatgaccgg cccggggggc tcggtgttcc atccggtgct gatcgaccag tctcggacga
      661 actgcttgtc cagcgaatgc tgctgctgcc cgggccgcca ggtgtcagca ggccagaagc
      721 gcgatgaatc cgacgtgagc gcttcatctc cgagcgtcaa tcgcccgtcg gtgtcccaac
      781 cgaactcgaa tttcgtgtcc gcgacgatga tgccgtttcc tgcagcgtgc tctgcggctg
      841 cggtgtaaag ctcaagggtc agatcacgga ggcgttgcgc agtatcgcgt ccggttccgc
      901 gcacgacgtc gtcaaaggtg atgaactcgt catgcccggt gtccgagtat ttggtggtcg
      961 gcgtgaagat cggctcgggt aaccggctcc ccgagaccag cccaggaggc aatgcaacac
     1021 ccgatacagt gccgaacttg tcgtactcac gcagacccag gccagcgagg tatccgcgag
     1081 caatgcactc cactgggatc atctgcagtg gcttggcacg gatggccctt ctcatgaatt
     1141 ccggcggcac gtcagtagcg gagatcagat ggttcggcac cacatcaccg agctggcgaa
     1201 accaccacac cgacagttgc gtcagcagcg cacccttatc gggaataggc gtcgggaggg
     1261 ccacgtcgta cagggagatc cggtccgagg cgaccagtag gatgtcgtcg ccgatcgaat
     1321 acaggtcccg caccttgccg gcggatatgt gctccacgtc gatcaccgta cccatcagct
     1381 ccgtcgggac ggtcacctgc ggagcagcat gtgggtaggt tggccgccat ggacgcccac
     1441 agcactttcc acgaccttgt tgggcagctc gattactcca tgttcatcat caccgtccgc
     1501 gccggcgacg agcaggctgg ttgcctggtc ggattctgca cccagagcag catcgatccg
     1561 ccgcgtttcc tggtctgcct gtcgcgcaag aaccgcactt accgggtggc gggcaggacc
     1621 gacctggtgg ccgtgcattt tgtgccacgg cgggccgggg cgctcgccga attgttcggc
     1681 ggccacagcg gtgatgacga ggacaagttc gcccgctgtg actggcagcc cggcccgggt
     1741 ggtcattctc accgccagtg ccgccgaact caacgactgc gccgcgcagg tcagttacct
     1801 gcggccggca
//
LOCUS       DATADN010000193         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1377734, whole genome shotgun sequence.
ACCESSION   DATADN010000193 DATADN010000000
VERSION     DATADN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377734"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            568..>1796
                     /locus_tag="VHH53_02565"
     CDS             568..>1796
                     /locus_tag="VHH53_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX2289086.1"
                     /translation="MGIQPKQLVWPLAAIVLASAFAVGLPAKPAPPSPLARAALGQGP
                     CTPEPATPKRQLRAMWIASVANIDWPSRPGLSAETVQAEYRAWLDLVQRHRHNAVVVQ
                     VRPTADALWPSPYEPWSEWLTGHRDGASPGWDPLAFLVAEAHQRNLEFHAWFNPYRVS
                     MPGGAGPDIGKLAPNHPARQRPEWAVAYPVNAAGSRLYYDPGNPEVRRFVEDAILDAV
                     ARYDIDGVHFDDYFYPYRAAGQDFADDATYVRYGAEFANKADWRRNNVNLLIQELGQR
                     IKGLKPWVKFGVSPFAIWRNRATDPIGSPTNGLQSSYDALYADTRLWVKQQWIDYIVP
                     QVYWNIGSGPADYSVVVPWWSDVVSGTRVQLYIGQADYKIGTTGAWLDPAEMSRHLAF
                     NRDHPEVLGDMHFSAKDV"
BASE COUNT          309 a          626 c          542 g          319 t
ORIGIN      
        1 aggcatgcgc gagggcgctg gcgccgacac cccagcattg catcccggag acggggtagc
       61 cggtcggctg tccgagttgg gcagcaccgg agtcggcgcc aaggacaggc cctgagcgaa
      121 tcactgttcg ggcaggcttc gcggcgtctg cgtgatcaac tctcacctca agaatgccac
      181 tgaccgcctc agccaagctg aaggcgactt ccgtgtcagc cctctctatt agctccgccg
      241 gccaccgggc cttcgtcggc cgacttcacc accggcttat cgccacatga gggcaccact
      301 ggcttgtcgt tcgctgacgg aaccggtggc tggtctgccg gcttcaccac tttggtcacc
      361 accatccgac ttcctccgtt ctccaccgtg aaggcagcac ttaagccctg tcctcaggtt
      421 agcgctgaag cggcggggtg gcagagttgc tgagagatgt cgccaatccc gtccgatttc
      481 cggcgagcat cagtgtggaa taaggtctcc gcggcccgac gcacccgagc ggcgtgacac
      541 gcccgttcca gtgaagggcg gcctgagatg ggtattcagc ctaagcagct cgtatggccg
      601 cttgctgcga ttgtgctggc cagcgcgttc gcggtcggcc tgccagccaa acccgcgccg
      661 ccctcaccgc tggcccgtgc cgcacttggt caaggcccgt gcacgccaga gcccgccacc
      721 cccaagcggc aactgcgtgc catgtggatc gccagcgtcg ccaacatcga ctggcccagc
      781 cggccaggcc tttccgctga gaccgttcag gccgagtacc gtgcctggct ggatcttgtc
      841 cagcgccacc gccataacgc ggtggtcgtg caggtacggc ccaccgcaga cgcattgtgg
      901 ccgtcaccct atgagccgtg gtccgagtgg ctgaccggcc accgtgatgg tgcgagcccg
      961 ggctgggacc ctcttgcgtt tctcgtcgcc gaggcccatc agcgcaatct ggagttccac
     1021 gcctggttca atccctatcg ggtgagcatg cccggcggcg ctgggccgga catcggcaag
     1081 cttgcgccca accatccggc tcgccagcgt cccgagtggg ccgtggccta cccggtcaat
     1141 gccgccggta gccgcctcta ctacgacccc ggtaacccgg aagtacgccg gttcgtcgaa
     1201 gatgccatcc tcgacgcggt cgcccgctac gacatcgacg gtgtgcactt cgacgactac
     1261 ttctaccctt accgtgccgc cgggcaggac tttgccgacg acgccactta cgtccgctac
     1321 ggcgctgagt tcgccaacaa agccgattgg cgccgcaaca acgtcaatct cctcattcag
     1381 gaactcggcc agcgcatcaa gggtctcaag ccgtgggtga aattcggcgt cagcccgttc
     1441 gcgatctggc gcaacagggc gaccgacccg atcggctcgc caaccaacgg gttgcaaagt
     1501 agctacgacg cgctctatgc cgacacacgg ctgtgggtca agcagcagtg gatcgactac
     1561 atcgtgcccc aggtgtactg gaacatcggt tccggcccgg cggactactc cgtcgtcgtc
     1621 ccgtggtggt ccgacgtcgt gtccggcacc cgggtacagc tctacatcgg ccaagccgac
     1681 tacaagatcg gcaccaccgg ggcgtggttg gaccccgccg agatgagtcg tcacctggcc
     1741 ttcaatcgcg accatccaga agtgctcggt gacatgcact tcagcgccaa ggacgt
//
LOCUS       DATADN010000194         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_18562, whole genome shotgun sequence.
ACCESSION   DATADN010000194 DATADN010000000
VERSION     DATADN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18562"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..692)
                     /locus_tag="VHH53_02570"
     CDS             complement(204..692)
                     /locus_tag="VHH53_02570"
                     /inference="COORDINATES: protein motif:HMM:NF042842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HEX2289087.1"
                     /translation="MIGRDVVALFPPALFIIACVLGLVGADPPGDSTGSGLAESLLLW
                     TLYLSIGWAGVGAGLSHTVFAKSTAKSIGWETNNFQYEIGFADFSTGVAAIYAVHVGS
                     PTAWAAVAIAGGLFRVLAGINHIRGIIRDKNYAPGNSLILIANFGPPIVMLITLLSTN
                     IL"
     gene            complement(859..1386)
                     /locus_tag="VHH53_02575"
     CDS             complement(859..1386)
                     /locus_tag="VHH53_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX2289088.1"
                     /translation="MATLSIWKFPSAEGAEDALRTLAQLQRDRLITVDDASVVSWQRD
                     RRKPRTWQARDLAGPGALSGAFWGLLFGLLFMLPLVGMAIGAASGAAAGSLSRIGLSD
                     EFLQRVRDQITPGTSALFVLTSDEVMDRVEEAFAGTSAELLVTNLSREQEERLRHAFD
                     AAGEHADGPGLSTSS"
     gene            1613..2227
                     /locus_tag="VHH53_02580"
     CDS             1613..2227
                     /locus_tag="VHH53_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2289089.1"
                     /translation="MSNVEAARLRNPRGEGSRLRAEVVRAARRVLEVSGVEDAVTLRA
                     TAREARVTAPALYGHFANRGEIIEAVISEAFAEFAAAVTDAMRGIADPVERLRAGCGA
                     YVRYAHEQPATYRILFTRHQPSAVPRAAAQAAEVFQLLVDTIEECVAAGRSASRDPAA
                     DAVVLWMALHGLAALPPSHPRFPWPEDHVLLDQVVNRSALLNGG"
BASE COUNT          378 a          756 c          797 g          343 t
ORIGIN      
        1 ggtggctcgc tggtggatac gtcggatccg tacacggatt ctgtgccgat gcctgccggg
       61 cccgccattg tcgcggtcac ggctgacggc gactgactcg atggacgtcg actttttgat
      121 gctgactaca gcgccggtgt caggacgcac atggaccgcc ggacctctgg tcggcgcggg
      181 tgtcgacgtg gaggtcccct cgttcagagg atgttggtcg agagcagggt gatcagcatg
      241 acgatggggg gcccgaagtt ggcgatcagg atcaacgagt tgccgggtgc gtagttcttg
      301 tcccggatga tgccgcgaat gtggttgatc cccgccagca ccctgaacaa cccaccggcg
      361 atcgccacgg ctgcccaggc agtgggcgac ccgacgtgca ccgcgtatat cgcggcgaca
      421 ccggtggaga agtcggcgaa gccgatctcg tactggaagt tgttcgtctc ccagccgatc
      481 gacttggcgg tgctcttcgc gaagacggtg tgtgacagtc ccgcgccgac cccggcccag
      541 ccgatgctca ggtacagcgt ccagagcagc aggctctcag cgagccccga acccgtgctg
      601 tcaccaggag gatctgctcc gacgaggccg aggacgcagg cgatgatgaa caaggcaggc
      661 gggaacaggg cgaccacgtc acgtccaatc acggggatcg tggggaaccc attgctcggc
      721 aggcgctccg cgttgcgcct ccgatcgagc ggagccacgt gcaccggcgt ccacgcgcac
      781 cccaagcggg tggcaccgac cacaccggag tggtcggatc tgtggcagga acaaccgctc
      841 ctgagccgag ctgacgcgtc aggacgacgt cgacaggccg ggaccatctg cgtgctcgcc
      901 tgcggcgtcg aacgcgtgcc ggaggcgctc ctcctgctca cggctgaggt tggtcacgag
      961 gagttcggcg gacgtgccgg cgaacgcctc ctccacccgg tccatcacct cgtcagaggt
     1021 gaggacgaac aacgctgagg tcccgggagt gatctggtcg cgcacccgct gcaggaactc
     1081 gtcgctgagg ccgatccgcg acagcgagcc ggcggcggca ccactggccg ctccgatcgc
     1141 catgccgacc aggggcagca tgaacagcag cccgaacagc aggccccaga acgctccgga
     1201 cagggcgccc ggtccggcca ggtcacgcgc ctgccaggtc ctgggcttgc ggcgatcgcg
     1261 ctgccaggac acgacggagg catcgtcgac cgtgattaga cggtcgcgct gcaactgcgc
     1321 caaggtgcgc aacgcgtctt cggcaccttc ggccgaaggg aacttccaga tgctgagggt
     1381 ggccactgga ggacctccag gtctgcggac tgtcggtggc ggggttgttg ctgggcacga
     1441 gaccggtgca gtgagatacg ggatctccgc acgcgccggt cgctcaagcg gaacaaccct
     1501 cgcgtgcgcc cgttcctacg gtcggcgtgc gctgccgacc gggcggctgt gtcgtaacgc
     1561 cgggagagaa caccactaaa caggtgtaaa gtcaaggggc gggaggatgt aggtgagcaa
     1621 cgtcgaggcg gcccgcctcc gcaatccccg aggagagggc tcgaggctgc gtgctgaggt
     1681 cgtccgggcg gcacgccggg tcctggaggt cagcggtgtg gaggacgcgg tcaccctgcg
     1741 ggccaccgcg cgggaggcgc gagtcacggc accggcgctg tacggccact tcgccaaccg
     1801 cggcgaaatc atcgaggcgg tgatcagtga ggccttcgct gagttcgccg ccgccgtcac
     1861 cgacgcgatg cgcggcatcg ccgatcccgt cgagcgcctc cgcgccgggt gcggggccta
     1921 tgtccgctat gcgcacgagc agccagcgac ctaccgcatc ctgttcactc ggcaccagcc
     1981 gagcgccgtg cccagggcgg ccgcgcaggc agccgaggtg ttccagctgc tcgtcgatac
     2041 catcgaggag tgcgtggcgg cggggcgctc cgccagccgt gacccggcag ccgacgcggt
     2101 cgtcctctgg atggccctgc acggcctggc tgcactgcct ccctcgcatc cccgcttccc
     2161 ctggcccgag gaccacgtgt tgctcgacca ggtggtcaac cggtcggcac tgctcaacgg
     2221 aggttgaccc accatcgggc ggactcgagc cagcgcccgg cgtacacagc gctg
//
LOCUS       DATADN010000195         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_928028, whole genome shotgun sequence.
ACCESSION   DATADN010000195 DATADN010000000
VERSION     DATADN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928028"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..416)
                     /gene="rplS"
                     /locus_tag="VHH53_02585"
     CDS             complement(60..416)
                     /gene="rplS"
                     /locus_tag="VHH53_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX2289090.1"
                     /translation="MNTLDALDAMSLRSDIPDFRPGDTLKVHVRVIEGSRQRVQVFQG
                     AVIRRQGGGIRETFTVRKVSFGVGVERTFPLHTPNIAKIEVVTRGDVRRAKLYYLRDL
                     RGKAAKIKEKRDAVAR"
     gene            complement(598..1371)
                     /gene="trmD"
                     /locus_tag="VHH53_02590"
     CDS             complement(598..1371)
                     /gene="trmD"
                     /locus_tag="VHH53_02590"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX2289091.1"
                     /translation="MLIDVVTIFPEYLTPAREALLGRAIERNLITFTVHDLRDWTRDV
                     HRAVDDTPYGGGPGMVMRPDVWGEALDEVLDGEPVPRLVVPSPAGRRFTQQVATELAE
                     QPRLVFACGRYEGIDQRVIDDAAERVVVDEISIGDYVLVGGEAAVLVMVEAIARLLPG
                     VLGNPASATQDSLSDGLLEGPCYTRPEVWRNREVPAVLRSGNHAAIARWRRDRAVERT
                     AQRRPDLLAALPPGALDSVDRALLEGLVSPSGYPSDGTG"
     gene            complement(1386..1874)
                     /gene="rimM"
                     /locus_tag="VHH53_02595"
     CDS             complement(1386..1874)
                     /gene="rimM"
                     /locus_tag="VHH53_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943618.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX2289092.1"
                     /translation="MVGRIGRPHGVRGEVTVQVRTDSPEQRFAVGAQLGAGAGHTLTV
                     DTVRPHAGNLLVRFAGVTDRAAAAELRGVLLTVDTVELPALDDPDEFYDHQLEGLAAV
                     GPDGAELGTVREVMHAPAGDLLVLETDRGEALVPFVRAIVPEVDLPGGRVVLDPPAGL
                     LD"
     gene            complement(1964..2203)
                     /locus_tag="VHH53_02600"
     CDS             complement(1964..2203)
                     /locus_tag="VHH53_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX2289093.1"
                     /translation="MSMLADALEHLVRGIVDNPDDVRVQLVTTRRGRTLEVHVHPDDL
                     GKVIGRGGRTATALRTVMSGIGGRGVRVDVVDTDR"
     gene            complement(2200..2709)
                     /gene="rpsP"
                     /locus_tag="VHH53_02605"
     CDS             complement(2200..2709)
                     /gene="rpsP"
                     /locus_tag="VHH53_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX2289094.1"
                     /translation="MAVKIKLMRLGKIRQPYYRIVVADARTRRNGKAIETIGKYHPKE
                     EPSLIEVDSARVQYWLGVGAQPTEPVQHILQVTGDWQRFTGQPGEEGTLRRAEPKPDK
                     AELFNRALAEAGEVPTTEATTPRRKSPRKADTAAATSPEPAPVTASEPVPAGADDQEG
                     TAKAADGQV"
BASE COUNT          462 a          982 c          912 g          455 t
ORIGIN      
        1 ggaacccgcc gggagcgcag ccggccggac cggctgtcag cgtgtcagcg cacggtcact
       61 cagcgagcga cggcgtcacg cttctccttg atcttggcgg ccttgccccg gaggtcgcga
      121 aggtagtaca gcttggcgcg ccggacatca ccgcgggtga ccacctcgat cttggcgatg
      181 ttcggggtgt gcagcggaaa ggtgcgctcc acgcccacac cgaaagacac cttacgaacg
      241 gtgaacgtct cgcggatccc tccgccctga cggcgaatga ccgcgccctg aaaaacctgc
      301 acccgctgcc gagaaccctc gatgacgcgg acgtgcacct tcagcgtgtc accgggccgg
      361 aagtcgggga tgtcggaacg cagcgacatc gcatccagcg cgtccagggt attcatcggg
      421 gatccgtcct cgtcatcgtg tgctctgcac ggtctcaggc gcccggagcg gacgcgaatc
      481 agtgacgtcg gtggcagcgg agtctcacca ccgtgggggc agtccctgcc ctgcttccgc
      541 tgcgggaacc ggtccagtgt gccagacgct gccgcccacc acgaaaccgg gcaattgcta
      601 accggtgccg tctgaggggt atccggatgg gctgaccagc ccctcgagca gtgcccggtc
      661 cacactgtcc agcgcaccgg gtggcagtgc agccaacaga tcgggacgcc gctgcgcggt
      721 gcgctccact gcgcggtccc ggcgccagcg ggcaatcgcg gcgtggttgc ccgaacggag
      781 cacggccggg acctcgcggt tgcgccagac ctcaggtcgg gtatagcaag gcccctccaa
      841 caaaccatcg gataatgagt cctgcgttgc cgaggcgggg ttgcccagca ctccgggcag
      901 caaccgggca atcgcctcga ccatgaccag cacggccgcc tcgcccccga cgagcacgta
      961 gtcgccgatc gagatctcat cgacgaccac ccgctcggcc gcatcgtcga tcacccgctg
     1021 gtcgatgccc tcgtagcgac cgcaggcaaa aacgagccgt ggttgctcgg ccagctcggt
     1081 ggcaacctgc tgggtgaacc gccgcccggc ggggctgggc accaccaacc gtggcactgg
     1141 ctcgccgtcg agtacctcgt ccaacgcctc accccagaca tcggggcgca tcaccatgcc
     1201 aggcccgccg ccatagggcg tgtcgtccac cgcacggtgc acatcgcgag tccagtcccg
     1261 caagtcgtgc accgtgaagg tgatcaaatt tcgctcgatg gctcgcccga gcagtgcctc
     1321 cctggccggg gtcaggtact cggggaagat ggtcacgaca tcaatgagca cagctgcgac
     1381 ctcgatcagt ccagcaaccc tgcaggtggg tcgagcacca ctcggccacc aggcaggtcc
     1441 acctcaggga cgatggccct gacgaaaggc actaacgcct cgccgcggtc tgtctccagc
     1501 accagcaagt cgccggctgg agcgtgcatg acctcgcgca ccgtacccag ctccgcgccg
     1561 tcgggcccga ctgcagccaa tccctctagc tggtggtcgt agaactcgtc agggtcatcc
     1621 agggcgggca actcgacggt gtcgacggtc aacagcacgc cgcgcagttc cgcggccgca
     1681 gcacgatcag tgaccccggc gaatcgcacg agcaggtttc ccgcatgcgg acggacagta
     1741 tccacggtca gtgtgtggcc agcgccagcg ccgagctgcg cgccgacagc gaatcgctgc
     1801 tccggcgagt cggtgcgcac ctgcaccgtc acctccccgc ggacaccgtg tggcctgccg
     1861 atccggccga ccacccgctg tgggtcagcg tccccagcgt gagagttgtc cccagcgtga
     1921 gagttactgc ctggctgcac ggtggagttt cctaccagca aggtcagcgg tcggtgtcga
     1981 cgacgtcgac ccgcaccccg cgcccgccga taccgctcat cacggtgcgc agcgccgtag
     2041 cggtgcgccc gccccggccg atcaccttgc cgagatcatc ggggtggacg tgcacctcga
     2101 gggtgcgacc tcgccgcgtg gttaccagct ggacgcgcac atcgtcaggg ttgtcgacga
     2161 ttcccctcac gagatgctcg agcgcatccg ccagcatgct cacacctgcc cgtccgctgc
     2221 cttcgcagtg ccctcctggt cgtcagcgcc ggctgggaca ggctcagacg ccgtgaccgg
     2281 ggccggctcc ggggacgtgg cagcagcggt atcagccttg cgggggctct ttctcctcgg
     2341 cgtggtggcc tcggtggtcg gcacctcgcc cgcctcggcc agcgcccggt tgaacagctc
     2401 agccttatcg ggcttgggct cggcacggcg cagagttccc tcctcgccgg gctggccggt
     2461 gaaccgctgc cagtcgccgg tgacctggag gatgtgctgt accggttcgg tgggttgggc
     2521 accaactccg agccagtact gcacccgtgc ggagtcgacc tcgatgaggc tgggttcctc
     2581 cttggggtgg tacttgccga tcgtctcgat cgccttaccg ttgcgacggg tgcgggcgtc
     2641 ggcgacaact atccgatagt agggctggcg aatcttgccg agtcgcatga gcttgatctt
     2701 tacagccacg ggtcgaacta ctcctgtcaa acgcacgagt ggtcacggtg agcgtgcacg
     2761 gcccatggtg gggacacgga ctgacgctgc ccataaggtc aggttcgttg c
//
LOCUS       DATADN010000196         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1224644, whole genome shotgun sequence.
ACCESSION   DATADN010000196 DATADN010000000
VERSION     DATADN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224644"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..728
                     /locus_tag="VHH53_02610"
     CDS             369..728
                     /locus_tag="VHH53_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2289095.1"
                     /translation="MATIELTKENFTDAVGEAELALIDFWASWCGPCRAFGPVFEEVS
                     DNNPDAVFGTVDTEAQRELAAAFRVMSIPTLVVMRQGLVLYAKSGALPQQALEELVGR
                     ARALDMDDVRRKRAAGR"
     gene            864..>1677
                     /locus_tag="VHH53_02615"
     CDS             864..>1677
                     /locus_tag="VHH53_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2289096.1"
                     /translation="MTAVAPQPIVTRPRPTRPTVKGSTLLGLFSTTDHKVIGMLYLVT
                     SMAFFFIGGSMAMLMRGELAQPGLQFLSNEQYNQLFTMHGTIMLLLYATPILFAFANL
                     VLPLQIGSPDVAFPRLNAFSYWAFLFGGLIVISGFLTPGGAADFGWFAYTPLSDAIHS
                     PGAGADLWIMGLVLAGLGTILGAVNMVTTVVCLRAPGMTMFRMPIFTWNILVTSLLVL
                     LAFPILTAALLGLEADRRLGAHVFDPDNGGVILWQHLFWFFGHPEVYIVALPF"
BASE COUNT          268 a          524 c          539 g          346 t
ORIGIN      
        1 cgagcgcagc gcctgacgcc gctaccgcgc gtgagttgcc ggcggtagga ccgttcgcgg
       61 tccccgcacc actgcagctc cttgttcgcc gcccaccggg tacgtacaga ccggaaggtg
      121 cacttcggca gggcggacgc cacggctggt tcgctggctg cggagctggt catagatcgg
      181 cgtctgactc acccgcaaca cgatagaggt gggccggtga cggtcggcat ccacatttgt
      241 gtacagctgg tgatgcctgt ggatgggcgg gatctcccgc cacggtgggt ggtcggaata
      301 ccaccggtgc gctcagcggt tcgcatggcc agaacttcag tatcttcagt accttcttcg
      361 gaggtgacat ggcgacaatt gaattgacaa aggagaactt caccgacgcc gtgggtgaag
      421 cagaactggc gctcattgat ttttgggcgt catggtgtgg gccgtgccgt gccttcgggc
      481 ccgtcttcga ggaggtatcg gacaataatc ctgacgccgt gttcggcaca gtcgacacgg
      541 aggcgcagcg tgaacttgcc gccgcgttcc gggtcatgtc gattcccaca ctcgtcgtca
      601 tgagacaagg tctggtgctc tacgccaaat caggcgcgct gccgcaacag gctctggagg
      661 agctggtggg tcgcgctcgg gcactggaca tggacgacgt gcgccggaag cgggccgcag
      721 gacggtagac gggctgaggg cagcggtgag cagggcagtg ctgcggctac tatctgtggg
      781 agcccgcgat gacgccgtca gcgcgggccg gtagcgtgca cgacgagtgc ccgatgcacc
      841 gcgaggcagc caggaggcaa cttgtgacgg ccgttgcgcc ccagccgatc gtgacacgtc
      901 cgcgcccaac gcgtcccacg gtcaagggtt cgaccctgct gggactcttc agcacgaccg
      961 atcacaaagt gatcggcatg ctctacttgg tcacctcgat ggctttcttc ttcatcgggg
     1021 gcagcatggc catgctgatg cgtggcgagc tggcccagcc gggcctgcag ttcctgtcca
     1081 acgagcagta caaccagctg ttcaccatgc acggcaccat catgctgctg ctttatgcca
     1141 ctccgatcct gttcgcgttc gcgaacctgg tcctgccgtt gcagatcgga tcgccagacg
     1201 tcgcgtttcc ccggctcaat gccttctcct actgggcatt cctcttcggt ggcctgatcg
     1261 tgatcagcgg cttcctcacg ccaggcggcg ctgctgactt cggctggttc gcttacaccc
     1321 cgttgtccga tgccattcac tccccgggcg caggtgcaga cctgtggatc atgggattgg
     1381 tcctggccgg gctgggcacc atcctgggtg cggtgaacat ggtcaccacg gtcgtctgcc
     1441 tgcgggcacc cgggatgacc atgttccgga tgccgatctt cacctggaat atcctggtca
     1501 cgtcactgct ggtgctgctg gccttcccga tcctgaccgc tgctttgttg ggcttggaag
     1561 ccgatagacg ccttggggcc cacgtattcg acccggacaa cggcggggtc atcctctggc
     1621 agcacctgtt ctggttcttc ggccaccccg aggtctacat cgtggcgctg ccgttct
//
LOCUS       DATADN010000197         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1224656, whole genome shotgun sequence.
ACCESSION   DATADN010000197 DATADN010000000
VERSION     DATADN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224656"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VHH53_02620"
     CDS             complement(<1..326)
                     /locus_tag="VHH53_02620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2289097.1"
                     /translation="MSTTAHLITRTSSVGLAFRPDIAGLRTVAVGVVVLFHAGVPWLP
                     GGYVGVDVFFVISGYLMTALLMKEIDQTGRVRLLAFYARRIRRLLPAAAVVLVATIVA
                     AKVLLTP"
     gene            complement(557..1891)
                     /locus_tag="VHH53_02625"
     CDS             complement(557..1891)
                     /locus_tag="VHH53_02625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2289098.1"
                     /translation="MAVSLGLHKWWGQSLGYDLRVALGLLLLALTAAALGLTRAWDPP
                     ILGQGSEEFSRLLRALATVAVLIGLVGLALKLPAVRPYVFGVIPIAFILAAGGRLALR
                     KVLHWRRRNGACMHQVLAVGTEQAVAELIVRTRRAAHNGWEVTAACTPFGAGANGDRS
                     ILEVPVVGDLDAVGKVAWNGRHRVVSVAQTPGWSSQRLHHLAWDLEGTGVELVVDPGL
                     MEIAGPRLHVAAVDGLPLLRLTEPKFTGLPRVIKAVGDRVTAGLLLLLLAPVLVAVAI
                     AVRSDGGPAFYRQLRVGQHGKHFAMIKFRSMVVNADQLRSDFADGNQGCGPLFKLRQD
                     PRVTRVGAFLRRHSLDELPQLINVLFGSMSIVGPRPPLPEEVACYSRDAQRKLLVKPG
                     MTGLWQISGRSDLSWEESVRLDLRYVENWSLALDALILWKTAGAVVRGTGAY"
BASE COUNT          434 a          720 c          695 g          377 t
ORIGIN      
        1 ggtgtcagca gaaccttggc ggcgacaatg gtcgctacga gcacgacagc agcggccggg
       61 agcaaccggc gaatccgccg cgcgtagaag gccagaaggc gaacccgccc ggtctgatcg
      121 atttctttca tgagcaaggc ggtcatcaga tacccggaga tgacgaagaa gacgtccacg
      181 ccgacgtatc cgccgggcag ccaaggcaca ccggcgtgga atagcacaac aacaccgacg
      241 gcgacggtcc gcagcccggc gatgtccggt cgaaacgcca ggcccaccga ggacgttctc
      301 gtgatcagat gtgcggtggt gctcatgccg actgactcgt ggtgtgccgc gacctgttac
      361 agcgccatga tcgcgagtgt ggcaggcaca ccgggaagtg ctccatgata ttggtgatgg
      421 tgagcgcacc catcgcctgt ttgggtgcga gcctaggaca cgcatgggga ctcgtcgtca
      481 cggcgttgcc tgccttttcc cgcagtcggc gcacggagac agggtattta cgcccgcggg
      541 tggcacgtaa accgtttcag taggcaccgg tgccccgtac gactgcacct gcggtcttcc
      601 agaggatcaa ggcatccagg gcgagagacc agttctccac atagcgtagg tccagccgca
      661 ccgattcctc ccacgacagg tcgctgcggc cgctgatctg ccacagccct gtcatccccg
      721 gtttgacgag caattttcgt tgcgcgtccc gtgagtagca ggcgacctct tcaggcagcg
      781 gcgggcgggg cccaactatg gacatcgacc cgaagagcac attgatgagc tggggcagct
      841 cgtcgagcga gtgcctccgg agaaacgcgc ccactcgggt gacgcgtggg tcttgacgca
      901 gcttgaacag tggaccacag ccctgattgc cgtcagcgaa gtcagacctg agctgatcgg
      961 cgtttaccac catggaccga aatttgatca ttgcgaagtg cttgccatgt tgacctactc
     1021 gtaactggcg gtagaaggct ggcccgccgt cactgcgcac cgcgatcgcc acagcgacta
     1081 ggaccggtgc gagcagcagt agcagcagac ccgccgtcac ccgatcaccc accgccttga
     1141 ttacgcgggg caatccagtg aacttaggct cggtgagccg cagcagcggt aggccgtcca
     1201 cggcggcgac gtgcagcctg ggaccggcaa tctccatcag cccgggatcc acgaccagct
     1261 cgacgcccgt gccctcgagg tcccaggcca ggtggtgcag ccgctgtgag ctccaacccg
     1321 gtgtctgggc gaccgagacc acgcggtgcc ggccgttcca cgcgaccttc cctaccgcgt
     1381 ccaggtcacc aacaacaggc acctcgagga tgctgcggtc gccgttggcg ccggcaccga
     1441 agggtgtgca ggcggctgtc acctcccacc cgttgtgggc ggcccgtcga gtgcgcacga
     1501 tgagctcggc caccgcttgc tcggtgccaa ccgcaagaac ctgatgcatg caggcaccat
     1561 tgcggcggcg ccagtgcaac accttgcgca gagctaggcg accgcccgcg gccagaatga
     1621 acgcaatcgg gatgacaccg aatacgtacg gtcgcacagc gggcagcttc agcgcgaggc
     1681 ccaccagtcc gatcagcact gcgactgtgg cgagcgcgcg cagcagccgg ctgaattcct
     1741 cggagccttg ccccaggatt ggcgggtccc aggctcgggt tagaccgagg gccgcagccg
     1801 tgagcgccaa caggagcagg ccaagtgcga cccgtaggtc atatccgagc gactggcccc
     1861 accacttatg cagcccgagc gatacagcca tcgccgccaa gtccgcgatg gccaccgatc
     1921 gggcgtatcg gatctcccac agtggtcggg tgatcgcctt acgcaatggg cgggtagctg
     1981 caccattgac atcatgagcc ggtgatagag cgctcggtat tgagttcgcc actatgggat
     2041 tcgttacttt tccagccaac tcgccgacgt tcgtgttcct gccaccacgc ccgaagccca
     2101 tgaatcatcg cctacattcg ctccgccgca aggattggat actgtcagtg acttcctgtc
     2161 tcggtggacc actcacctga gtggctttgt cggagagtcg aagtcgacat tggtaacgtt
     2221 acggtc
//
LOCUS       DATADN010000198         2331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_173236, whole genome shotgun sequence.
ACCESSION   DATADN010000198 DATADN010000000
VERSION     DATADN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173236"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VHH53_02630"
     CDS             complement(<1..538)
                     /locus_tag="VHH53_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2289099.1"
                     /translation="MFKLAKSSAVKARTQTINQLKAVLVAADPMLRETLSGLSNLLLI
                     RRCTQLDLDTPCDTTSAAAYTLRLLARRIQALTSEIRELEQQITAAVTRHTPQLLTRC
                     GIGPDNAAALLIAAGDNPDRLRSEASFAALCGVSPLEASSGRTSRRQLNRSGDRQANS
                     ALYRIALSRLRWDARTRDY"
     gene            531..722
                     /locus_tag="VHH53_02635"
     CDS             531..722
                     /locus_tag="VHH53_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289100.1"
                     /translation="MNTPQHLHRPVTGFGAAAGPAAQHCAGGSFSVEGVGLAPSTTGG
                     LVRLVDLNHLDTGCLQVPG"
     gene            complement(1568..>2331)
                     /locus_tag="VHH53_02640"
     CDS             complement(1568..>2331)
                     /locus_tag="VHH53_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HEX2289101.1"
                     /translation="AAGPGDYAHQVLAYEHGSVIDGRTVTTVLPTDIITVGDEMYLHV
                     MVCEGLGNVHWTELHCSSDNGETWRHTGVTWPGGHHGGLFQMLTCEHGDDGYVYVFST
                     GFQRDKGLVLQRVPADRFTEPAAWQSWGYRDGQWAWGNPPTVALSGKFGELCLRRVEG
                     RWLLALFDAGSYRIDVLSLESPTANLYDAQRQTLVHGTSWDTEDHAKGQVAQLYGGYL
                     VPGSTLDDLHLVVSQWNTTINWPYRAMQFHANVAD"
BASE COUNT          400 a          729 c          787 g          415 t
ORIGIN      
        1 ggtagtcgcg ggtgcgagcg tcccagcgca gtcgtgacag ggcgatacgg tagagcgcgg
       61 aatttgcttg tcggtcgccg ctacggttaa gctggcgacg gctggtcctg cctgaggacg
      121 cctccagggg gctgacaccg cacagggccg cgaaggaggc ctcgctgcgc agccggtcgg
      181 ggttgtcacc ggcggcgatg agcagggcgg cggcgttgtc ggggccgatc ccgcagcggg
      241 tcagcaactg gggagtgtgg cgggtgacgg ctgcggtgat ctgttgttcc aattcgcgaa
      301 tctcactggt gagcgcctgg atgcgccggg ccagcagacg cagggtgtag gcagcggcgc
      361 tggtggtgtc gcagggtgtg tctaggtcta gttgcgtgca gcgacggatg agcagcaggt
      421 tgcttagccc ggagagtgtt tcgcgcagca tggggtcggc cgcgacgagc acggctttga
      481 gttggttgat cgtctgggta cgggccttga cggccgagga tttggccagc ttgaacaccc
      541 cgcagcatct ccaccggccc gtcaccggct ttggcgctgc cgctggcccg gccgctcagc
      601 actgcgcggg cggcagcttc agcgtcgagg gtgtcggtct tgccccgtcg acgacgggtg
      661 gccttgtccg gctggttgac ctcaatcacc tcgacaccgg ctgcctgcag gtgccgggct
      721 agtgccccac cgtaggagcc ggtgcactcc actccggctc gccgcaccgt gccgaagcga
      781 cccgcccagg ccagcaatgc ctggtagccg gccgcggtgg tcgggaacat ctcgctgctc
      841 agcagcacac ccaatgggtt gatcaccgct gcagcgtgca gatccttatg ggtatccaca
      901 ccgagaatca cctcgctagc agtgttgtca tcctggtcgt gatgaatcgg ctggggcatg
      961 ctggacacgg gtcgtctgtc tcctgatcgc tacgggaatg gatggccgtc gccgggccgg
     1021 tgtgggcggt cagaactgtg acggtgccct gttgacggca aggcccctat cgggacacgc
     1081 ccacccggtc cggcgacagc aagcaccacc ccaagccatg gtcgacagat cagagccaag
     1141 gcattcagcc agttgcatta cgggtcagac catggcctgg gacggcacgt cggcgatctt
     1201 cacagtcaga ccaagctccg aggcggcaca cgccgatcct cacagatgca ttaacgcgaa
     1261 ggacgtcctc gccaggcccc aaaaggggag gcgcgatccc tcacgtgcct tgagaaccgt
     1321 aacaaaccgg gcattcgaag gcctgctacg tggcttctgg gcaggcaggt ggaccagctc
     1381 aggtgggcgg acgctcatgc aacagtgcct ccccaggtat cgccgcacgc ctccacccgg
     1441 cccggtgagc tcatcgacaa tccgcgcgac cgttcgtcgc atgatcggtc gccgacgagc
     1501 accacggcga acggacagac agcgtcggct ggacagacat ggaaggcatc tttgtgtgtc
     1561 tcgtggttca gtcggcgaca ttcgcgtgga attgcatggc gcggtatggc cagttgattg
     1621 tggtgttcca ctggctgacg accaggtgca gatcgtcgag cgtcgagccg ggcacgaggt
     1681 agccgccgta gagctgagct acctgccctt tggcgtggtc ttcggtatcc catgaggtgc
     1741 catgcaccag cgtctgccgc tgtgcgtcgt acaagttcgc cgttggtgac tccagactca
     1801 aaacatcgat gcgataactg cctgcgtcga acaacgccag cagccagcgg ccttcgacgc
     1861 gacgcagaca cagttcgccg aacttgccgg acagggcaac ggtcggggga ttgccccatg
     1921 cccactggcc gtcacgatat ccccagctct gccaggcagc cggctcggtg aaccgatcgg
     1981 ccgggacccg ttgcagcacc aggcccttgt cgcgctggaa tccggtggag aacacataca
     2041 catagccatc gtcaccgtgt tcgcaggtca gcatctggaa caggccaccg tggtggccgc
     2101 ctggccaggt gacgccagta tgcctccagg tctcgccgtt gtcgctcgag cagtgcagct
     2161 cggtccagtg gacgttgcca aggccctcgc acaccatcac gtgcaggtac atctcgtcac
     2221 cgaccgtgat gatgtcggta ggcaggaccg tcgtgaccgt gcggccgtcg atgactgagc
     2281 cgtgctcata ggccagcacc tggtgggcgt agtcgccggg gccggcagct g
//
LOCUS       DATADN010000199         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1078896, whole genome shotgun sequence.
ACCESSION   DATADN010000199 DATADN010000000
VERSION     DATADN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078896"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="VHH53_02645"
     CDS             <1..1853
                     /locus_tag="VHH53_02645"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2289102.1"
                     /translation="EAAATLARLCARLPLALRVAAELAAARPTISLAQLVDELADEQR
                     RLELLDAGGDARTAVRGVFSWSYQHLPAPAAHAFRLIGLHPGPYFDSYAAAALANTSL
                     DQAQQMLDLLSRAHLIQAPHPGHYGIHDLLRAYATWLADVEDSKAEQRAGLTRLFDHY
                     LATAAAAMDTLIPAEQHRRPRIPLPATPIPPMPDRATARTWLDSERVTLAAVSAYTAA
                     RGWLDHTTQLSTTLYRYLDASGHFPDGLTIHTHALHAARHIDKPAAEADALLNLGHIY
                     WQQGHYGKATHHLQQALTLASEIGDRVGESYALLNLGIVYQRQGHYRQATHHLQQALT
                     LASEIGDRVGETNALLNLGIVDRRQGHYQQATDHYQQALTVASRVGDRHGEANALDNL
                     GTIYQRQGHHELAADHHQQALALFHEIGDRHGEANTLLDLGTVYRRQGHYQQAADHHQ
                     QALALFREMGDQHGEACALNRVGETLVAIRQADQALTQHTAALALATQIGDRYEQARA
                     HNGLAHTHQLTGDLDQALAHQTAALALASQIGEPYEHARAHNGLAHTHQVTGDIDQAR
                     HHWHHALALYTDLGVPEADDVHAHLTALDQAAGDDNLDQGKHLSGNPLKP"
     gene            complement(2099..>2620)
                     /locus_tag="VHH53_02650"
     CDS             complement(2099..>2620)
                     /locus_tag="VHH53_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2289103.1"
                     /translation="LALLVAIAVVYMVMVVTFRSLLQPLVLLVAIPFAATGALGLLLL
                     TDTALDVAALIGMLMLVGIVVTNAIVLIDRVNQYRRDGRELFDALVEGAVQRLRPILM
                     TALATVFALLPMALGLTDGGVFISQPLAIVVIGGLISSTVLTLVLVPVLYLLAERKRG
                     QHRQRTADELVTD"
BASE COUNT          509 a         1010 c          730 g          371 t
ORIGIN      
        1 ccgaagcggc cgccacgctg gccaggctgt gcgcccgact gccattggcg ttgcgggtgg
       61 ccgccgaact cgccgctgcc cgtccgacca tcagcctggc gcagctggtc gacgagttgg
      121 ctgatgaaca gcggcggttg gaactgctgg atgccggtgg cgatgcccgc accgcggtgc
      181 gcggcgtgtt ctcctggtct taccaacacc tccccgctcc cgcggcgcac gcgtttcgac
      241 tgatcggcct gcaccccggc ccgtacttcg attcctacgc cgctgccgca ctggccaaca
      301 ccagcctcga tcaagcccag cagatgctgg acctgctgag ccgggcacac ctgatccagg
      361 ctccccatcc aggtcactac ggcattcatg acctgctgcg ggcttacgcc acgtggctcg
      421 ccgacgtcga ggactccaag gcggagcagc gggcggggtt gacccgcctg ttcgatcact
      481 acctcgccac cgctgcggcc gcgatggaca cccttattcc tgccgagcag caccgccgac
      541 cacgcattcc cctacctgca acgcccattc cgccgatgcc cgaccgcgct accgcacgga
      601 cctggctgga ttccgaacgg gttaccctcg ccgctgtcag cgcatacact gctgctcggg
      661 gctggctcga tcacaccact cagctgtcca ccaccctgta ccgctacctc gacgccagcg
      721 gccactttcc cgacggcctg accatccaca cccacgcgct gcacgccgcc cgccacatcg
      781 acaagcccgc tgccgaagcc gatgcgctgc tcaacctcgg ccacatctat tggcagcagg
      841 gccactacgg gaaggccacc caccaccttc agcaggccct caccctcgcc agcgagatcg
      901 gcgaccgagt cggcgaatcc tacgcgctgc tcaacctcgg catcgtctat cagcggcaag
      961 gccactacag gcaggccacc caccaccttc agcaggccct caccctcgcc agcgaaatcg
     1021 gcgaccgagt cggcgaaacc aacgcgctgc tcaacctcgg catcgtcgac cgccgacaag
     1081 gccactacca gcaagccacc gaccactacc agcaggccct taccgtcgcc agccgggtcg
     1141 gtgaccggca cggtgaagcc aatgcactcg acaacctcgg caccatctac cagcggcagg
     1201 gccaccacga gctggccgcc gaccaccacc agcaagccct ggcccttttc cacgagatcg
     1261 gcgaccggca cggtgaagcc aacacgctgc tcgatctcgg taccgtctat cgccggcaag
     1321 gccattacca gcaagccgcc gaccatcacc agcaggccct ggccctcttc cgcgagatgg
     1381 gtgaccaaca cggcgaagcc tgcgccctca accgcgttgg tgaaaccttg gttgccattc
     1441 gccaagccga ccaggccctc acccagcaca ccgccgccct agccctggcc acccagatcg
     1501 gcgaccggta cgagcaagcc cgcgcacaca acggcctcgc ccacacccac cagctcaccg
     1561 gcgatctcga ccaggccctc gcccaccaaa ctgcggccct ggccctggca agccaaatcg
     1621 gtgagccata cgagcatgcc cgcgcacaca acggcctcgc ccacacccac caggtcaccg
     1681 gcgatatcga ccaggctcgc catcactggc atcacgccct ggccctctac accgacctcg
     1741 gcgtccccga agccgacgac gtccacgctc acctcaccgc cctcgatcaa gcagcggggg
     1801 atgacaacct agaccaagga aagcacctca gcggcaatcc cctgaagccg tagagactga
     1861 acttggtgga tcttgtcatt cgtgcgggtg ccggctggca ctgaaccaac gctggtccac
     1921 tcgggtagca cgaaagtccc ggctcaccag atccggtgct ttccaccgcc cgcgagccga
     1981 tcgggtgctc cttcgccgcc atcggccggc tgttggccaa gctcgacacc aagatcgggt
     2041 gcaactcgtg atcgtgggat acgagaccgg cttggtcggg gccatcccta cgtagccgct
     2101 aatccgtgac gagttcatcg gctgtgcgct ggcggtgctg gccgcgcttg cgctcagcga
     2161 gcaggtagag caccggtacc aaaaccaggg tcagcaccgt cgagctgatc agcccgccga
     2221 tcacgacgat ggccagcggc tgcgagatga acaccccacc gtcggtgagc ccgagcgcca
     2281 tcggaagcaa ggcgaacacg gtcgccagcg cggtcatcaa gataggccgt agccgctgta
     2341 cggccccttc cacgagagcg tcgaacagct cgcgcccgtc acggcggtac tgattgaccc
     2401 ggtcgatgag cacgatagcg ttggttacca cgatcccgac gagcatcagc atcccgatca
     2461 gcgcggcgac atcaagcgca gtatcggtga gcagcaacag gcccagcgca ccggtggccg
     2521 cgaacgggat cgcgacgagc agcaccagcg gctgcagcag gctgcggaat gtcaccacca
     2581 tgaccatgta gaccacggcg atcgccacca gcagggccag
//
LOCUS       DATADN010000200         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_777270, whole genome shotgun sequence.
ACCESSION   DATADN010000200 DATADN010000000
VERSION     DATADN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777270"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VHH53_02655"
     CDS             <1..903
                     /locus_tag="VHH53_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX2289104.1"
                     /translation="LHVGSDIDDAPRRKVVEQDHGLDKALDNTLIALAEGALAGGEPV
                     RLDLPVRNVNRTVGTMLGSELTRRWGGDGLPDNTVDITLTGSAGQSFGAFLPRGITLR
                     LIGDANDYVGKGLSGGRITVRPHPDAQFTAEQHIIAGNVILYGATSGEMFLRGMVGER
                     FCVRNSGALAVVEGVGDHGCEYMTGGRVVVLGPTGRNFAAGMSGGTAYVLDLAVHRVN
                     QEMVDLDPLDDEDRAFLADAVALHHAETGSAVAHALLADWDTAVDRFGKVMPKDFKRV
                     LAAQAAAQRDGRDINEAIMEAAHG"
     gene            896..>1692
                     /locus_tag="VHH53_02660"
     CDS             896..>1692
                     /locus_tag="VHH53_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX2289105.1"
                     /translation="MADPKGFLTTGRQNPPRRPVDLRLRDWREVYEDFSREALEAQAG
                     RCMNCGIPFCHLGCPLGNLIPEWNDLVWRQDWADAIERLHATNNFPEFTGTLCPAPCE
                     AACVLAINDDAVTIKQVEIEIIDRAWDEGWVVPQPPAVRTGKKVAVVGSGPAGLAAAQ
                     QLTRVGHDVVVYERADRIGGLLRYGIPEFKMAKHRLDRRLEQMRAEGTEFRAGVDVGV
                     DVTAKELRASFDFVVLAGGATAWRDLPVPGRELTGVYQAMEYLPPSNR"
BASE COUNT          264 a          530 c          630 g          268 t
ORIGIN      
        1 ctgcacgttg gctccgacat cgatgacgct ccgcggcgca aggtggtgga gcaggaccat
       61 gggttggaca aggcgctgga caacaccctg atcgcgctgg ccgagggggc gctggctggc
      121 ggtgaaccgg tgcggctgga cctgccggta cgcaacgtca accgcacggt gggcaccatg
      181 ctcggctccg agctcacccg tcgctggggt ggcgacgggt tgcccgacaa caccgtcgac
      241 atcacgctga caggatcggc cggccagtcc ttcggggcgt tcctgccgcg cgggatcacc
      301 ctgcggctaa tcggcgacgc caacgactac gtcggcaagg gcctgtccgg cgggcggatc
      361 acggtccgtc cacaccctga cgcgcagttc accgcggagc agcacatcat cgcgggcaac
      421 gtgatcctct acggggcgac gtccggggag atgttcctgc gtgggatggt cggggagcgc
      481 ttctgcgtgc gcaactccgg tgcgctggcc gtcgtcgagg gcgtcggtga tcacggctgc
      541 gaatacatga ccggtggccg ggtggtggtg ctcgggccga ctgggcgcaa cttcgcggcg
      601 gggatgtccg gcgggaccgc ctatgtgctc gacctggcgg tccatcgagt caatcaggag
      661 atggtcgacc tggatccgct ggatgacgag gaccgcgcgt tcctggccga cgcggtggcg
      721 ttgcatcacg ccgagaccgg gtcggcggtg gcgcatgcgc tgctggccga ctgggacacc
      781 gcggtggatc ggttcggcaa ggtcatgccc aaggacttca agcgggtgct ggccgctcag
      841 gcagcggcgc agcgggacgg ccgcgacatc aacgaggcca tcatggaggc cgcccatggc
      901 tgacccgaaa ggtttcctca ccacggggcg ccagaacccc ccgcgtcgcc cagtcgacct
      961 acggttacgg gactggcgag aggtctatga ggacttcagc cgtgaggcgc tggaggcgca
     1021 ggccggccgg tgcatgaact gcgggatccc gttctgccac ctcggttgcc cgctgggcaa
     1081 cctcatcccg gagtggaacg acttggtctg gcgccaggac tgggctgatg ccatcgagcg
     1141 gttgcacgcg accaacaatt tccccgagtt caccgggacg ctgtgtccgg caccgtgcga
     1201 ggcggcctgc gtgctggcca tcaacgacga cgcggtgacc atcaagcagg tcgagatcga
     1261 gatcatcgac cgggcttggg acgagggttg ggtggtcccg caaccacctg ccgtgcgcac
     1321 cggtaagaag gtcgccgtgg tcgggtccgg tccggcgggg ctggccgcgg cccagcagct
     1381 gacccgggtc gggcatgacg tggtggtcta cgagcgcgcg gaccggatcg gtgggctgct
     1441 gcgctacggc atccccgagt tcaagatggc caagcaccgg ttggaccggc ggctggagca
     1501 gatgcgcgcg gagggcaccg agttccgggc cggcgtcgac gtgggagtgg acgtcacggc
     1561 caaggagcta cgcgcgagct tcgattttgt cgtgctcgcg ggtggtgcta ccgcgtggcg
     1621 ggacctgccg gttccgggac gcgaattgac cggcgtgtac caggcgatgg aatacctgcc
     1681 gccgtccaat cg
//
LOCUS       DATADN010000201         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1378549, whole genome shotgun sequence.
ACCESSION   DATADN010000201 DATADN010000000
VERSION     DATADN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378549"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..828
                     /locus_tag="VHH53_02665"
     CDS             76..828
                     /locus_tag="VHH53_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2289106.1"
                     /translation="MPAPLLLETHYQGHTLVIRPVGDLTPESHARLRDGLLKYAADEP
                     PEIVVDLANMRVAIASLLTVFPTVRNRINDWPGVLMVLAGARQPLRTLLDTSAVPRLV
                     PTYPSVGEALQALEAAPRRRRCQVKLTCDPASAQQVRRLVKQTSHEWRVPGTVIDAAV
                     VVASELTDNMVYHARSDGWLRLELRGNMFTVAVADADSRPPKLRVPGQRGAGGRGLVL
                     VDKLSRTWGTAPQSSGGKVVWALLTVPPGKRR"
     gene            1117..2301
                     /gene="dnaN"
                     /locus_tag="VHH53_02670"
     CDS             1117..2301
                     /gene="dnaN"
                     /locus_tag="VHH53_02670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX2289107.1"
                     /translation="MEFELERDAFAEAVAWTARGLPARPVVPVTAGVVLEVTDGAMIV
                     SGFDFEVLNRIEVPVTDKAAGRVLVSGRLLAEIARALPPKDVRVAVEGNRLAVRCGSA
                     RFNLPLLHVEDYPAVPELPATSGRVRGDVFATAVSQVTAAASRDETLPMLTGVRMEIG
                     PDALTFAATDRYRLAERRIPWTPSPSGPPLDSASAESMLDPASRPSVIVPAKTLADAA
                     KSLGSASEVLLSMGNATDAGFLGFSAGGRQTLTRLLDGEFVKFASLWPTEVTTTVEVE
                     VSPFVAALKRVSLVAQRFAPVRLAFAEGGVQLSAEADDESSAEEEVEVGITGEPLTIG
                     FNAGYLLDGLSGVTTARATLSFTAPSRPTVISSLTGLDTDEPDNGEGSYRYLVMSMRL
                     RG"
     gene            complement(2276..>2688)
                     /locus_tag="VHH53_02675"
     CDS             complement(2276..>2688)
                     /locus_tag="VHH53_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289108.1"
                     /translation="DVLADRLGVTLVVVSYVVAHPAAVAPAAVFLIQFVVLDAYLTLT
                     FTRWSLLSPNYFNLVDATVYRLNWSMPAKATNTGAVVLLWLITESPMAATVLAAAVLG
                     VKLFSTARVARLPVPRTLSGCAVLDAASRAAAST"
BASE COUNT          429 a          883 c          925 g          451 t
ORIGIN      
        1 acgaacgata gtgctgagct gctggaaccg gcttctaccg ctcgacgtag atatcgtggg
       61 gatggagagt ataacatgcc cgccccgcta ttactagaaa ctcattatca gggccatacc
      121 ctggtgatcc gaccggtggg agatctgact cctgagtctc acgcgcggct gcgggacggc
      181 ctgctcaagt acgcggccga cgaaccgccg gagattgtgg tcgacctggc taatatgcga
      241 gtggcgatcg cgtcgttgct gactgttttc cccaccgtgc gcaaccgcat caacgactgg
      301 cccggagtgc tgatggtgct ggccggggcg cggcaaccgc tacgcactct gctcgacacc
      361 agcgccgtac cgcggctggt cccaacctat cccagcgtcg gcgaagcgct ccaggccctc
      421 gaagcggcac cacgccgccg ccggtgtcag gtgaagctga cctgcgatcc ggccagtgcg
      481 cagcaggtca ggcgactggt caagcagacc tctcatgagt ggagggtgcc gggaacggtc
      541 atcgatgccg cggtggtggt ggcctcggag ctgactgaca acatggtcta tcacgcgcgt
      601 tcggacggct ggttgcgtct ggagctgcgc ggcaacatgt tcaccgtcgc ggtggccgac
      661 gcagactcgc ggccaccgaa gttgcgagta cctggtcaac gcggcgccgg tggccgcggc
      721 ctcgtactcg tcgacaagtt gagccggaca tggggtaccg cgccccagtc ttccggcggc
      781 aaggtggtgt gggcgctgtt gaccgtcccg cccggtaagc gccggtagcc gccaggcagg
      841 tgtcaggatt gcctgacaag gggttctccc ttggtgtcag gggtcgtatc gtccgtggtg
      901 gagcccgtct ccggcccgga tgggccgaat atagggaccg ccgaccgggt atcagggggg
      961 ctgggtggtg cccggtcggc ggccagtacg ccctgatggc ttcgtgctgg cacccaccga
     1021 tcgcacgctg accaccaccg ccgcgccgcg acaacgtccc ggtacgctgg cgcacccggt
     1081 gcaaccggga gtactcgcag ggacggtggt ttcaacgtgg agttcgagct tgagcgggat
     1141 gcgttcgccg aggcggtcgc gtggacggcg cggggcttgc cggcccggcc cgtcgtcccg
     1201 gtcaccgcgg gcgtggtgct cgaggtaacc gatggtgcga tgatcgtctc cggattcgac
     1261 ttcgaggtgc tcaaccgcat cgaggtgccc gtcacggaca aggccgctgg acgcgtgctg
     1321 gtgtccggtc ggctgctggc cgagatcgcc cgcgcgttgc cgcccaagga cgtgcgggtg
     1381 gccgtcgagg gaaaccggct ggcggtgcgg tgcggctcag cccgattcaa cctgccgctg
     1441 ttgcacgtcg aggactaccc cgcggtgccg gagctgcccg ccacctcggg gagggtgcgc
     1501 ggtgacgtct ttgccaccgc ggtctcgcaa gtcaccgcgg cggccagccg ggacgagacg
     1561 ttgcccatgc tcactggagt gcgcatggag atcggtcccg acgcgctgac cttcgccgcg
     1621 accgaccggt accgcctcgc cgaacggcgc atcccatgga caccgtcgcc gagcggcccg
     1681 cccctcgact ctgcaagcgc ggagtcaatg ctcgaccccg caagcaggcc ttcggtcatc
     1741 gttccagcta agacgctcgc tgatgccgcc aagtccctcg gctcggccag cgaggtgctg
     1801 ctgtcgatgg gcaacgccac cgacgcgggc ttcctcggtt tctccgcggg cggacgccag
     1861 acgctgaccc ggctgctgga tggggaattc gtcaagttcg cttcgctgtg gcccactgag
     1921 gtcaccacta ccgtggaggt ggaggtcagc ccgttcgtgg cggcgctcaa gcgggtgtcg
     1981 ctggtcgcgc agcgcttcgc accggtccgc ctggccttcg ccgagggcgg cgtccagttg
     2041 tccgcggagg ccgacgacga atccagtgcc gaagaggaag tcgaagtcgg tatcaccggt
     2101 gaaccactga ccatcggctt caacgctggg tacctgcttg acgggctcag cggggttacc
     2161 actgcgcgag cgacgctgtc gttcaccgcg ccgtcgcgcc ccaccgtcat ctcctcgttg
     2221 accggactcg acaccgatga accggataac ggggagggct cctaccgcta tctcgtcatg
     2281 tcgatgcggc tgcgcggcta gccgcgtcga gcaccgcgca gccggacagc gtccgtggca
     2341 cgggtaaccg tgctacccgt gcggtactga acagcttcac cccgaggacc gctgcggcca
     2401 gcaccgtcgc tgccatcggc gattcggtga tcagccacag gagcaccacc gcgccggtgt
     2461 tggtggcctt cgcgggcatt gaccagttca gccggtacac cgtggcgtcg accagattga
     2521 agtagttcgg ggacagcagc gaccagcggg tgaaggtcag cgtgaggtac gcgtcgagta
     2581 ccacgaactg gatcaagaac accgcggcag gtgctaccgc agccggatgg gcgaccacgt
     2641 agctgaccac caccagggtc acgcccaacc ggtcggcgag tacgtcga
//
LOCUS       DATADN010000202         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_475275, whole genome shotgun sequence.
ACCESSION   DATADN010000202 DATADN010000000
VERSION     DATADN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475275"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..980)
                     /locus_tag="VHH53_02680"
                     /pseudo
     CDS             complement(109..980)
                     /locus_tag="VHH53_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1082..1393
                     /locus_tag="VHH53_02685"
                     /pseudo
     CDS             1082..1393
                     /locus_tag="VHH53_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873747.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1388..1518
                     /locus_tag="VHH53_02690"
                     /pseudo
     CDS             1388..1518
                     /locus_tag="VHH53_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          217 a          514 c          556 g          231 t
ORIGIN      
        1 ctgagcagct cggtgagcag gtggcgccgt agtcgaacgc gccggtcccc gcacgcccag
       61 caagaccgcc acctaagcaa caccgccacc tagggcctgt cccgcggatc atgtgctgag
      121 ccagagcact gcgctgacca gcagcagcgt ggcgcgatag taggcggcgc gcttggcgta
      181 gcgggtggcc agggcgcgga actgcttgag ccggttgaag cagcgttcca cgacgttgcg
      241 ctgcttgtag cggtcacggt cgaaggtcgg tggtcgtccg ccggccgagc ccaggcctcg
      301 ccggcgcgcc ttctggtcgc gcttttccgg aatcgtgtgc ccgatgccgc ggcggcgcag
      361 cgcccgacgg gtcgagggat gggagtaggc cttgtcggcg atcaggtgag cggggcgctt
      421 gcgcggtcgg cctggtccga tccgaggcac gcggatgtcg tccagcaggg gcaggagctg
      481 gggattgtct ccggcctgcc cgggagtcaa ccgcaccgac agcggccgac cgcggccgtc
      541 gacggccagg tgcaccttgc aggtcagccc gccgcgggag cgccccaacg cctccccgtc
      601 gacggcgagg gcctcgaccg gagcgctgca gccccctttt tccgggcacc agcggcgtgc
      661 tggtgggccc ggacgctgga ggaatccacg ctgatcgtcc actcgaccgc gccgacggcg
      721 tcgtccttga cgaccacgtg ctccaggatg cgttcccagg tgccatcggc ggtccacttc
      781 cgcagccgct catgcacggt cttccacggc ccgtagcgct cgggcaggtc acgccagggc
      841 acaccggtgt gggcctgcca caggatgccg ttgatcacct gccggtgatc cctccaccgc
      901 ccgccccgac caccggtcgc cggcagcaac ggctggatgc gggcccagga cgcgtcggtg
      961 agctcgtgtc ggccaaccat cactcgagcc tgaaccacac cgatgaaaga tcgccaggac
     1021 aggccctagt agtacttcgt taggtcttgt ctgacctgcg tccgcgcgct gccatggggg
     1081 catgactccg ggggagctgg ccgccgtccg agggcggttg gaggagttcg cggcggaggt
     1141 gttcgcgccg ctggcccgcc gtgatcagcg ggacaagggc gtgacctacg tgcgcgggct
     1201 gctgctggac gggcggcgca agtcgatgct gccgatggcc gaacggctgg gcgtggatca
     1261 tcagggcctg cagcagttcg tctcctcctc gacctgggcg gtcgagccgg tgcgtgagcg
     1321 gctggcccgc agggcggtga cggtgatcga cccggatgcg tgggtcatcg acgacaccgg
     1381 gttcgtcaag gacgacgcgg tcggcgcggt cgagtggacg atcagcgtgg attcctccag
     1441 cgtccgggcc caccagcacg ccgctggtgc ccggaaaaag ggggctgcag cgctgcggtc
     1501 gaggccctcg ccgtcgac
//
LOCUS       DATADN010000203         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_18954, whole genome shotgun sequence.
ACCESSION   DATADN010000203 DATADN010000000
VERSION     DATADN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18954"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VHH53_02695"
     CDS             complement(<1..454)
                     /locus_tag="VHH53_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2289109.1"
                     /translation="MITIGIDPHKSSLTAVVVESTREPVATIRVAVTATTVRQLRQWA
                     ARWPQRRWAIEGAEGLGRGVAQGLAGAGEQVVDVPAQLAARARLLNSGHARKTDALDA
                     ASVAAVAVHHRRLRLVRAEGHSVGLRLLSDRRDDLAEERTHTVNRLHAL"
     gene            917..2389
                     /locus_tag="VHH53_02700"
     CDS             917..2389
                     /locus_tag="VHH53_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX2289110.1"
                     /translation="MTTGIKTAKATNQMIYDVGTRPVAAHAPTSTAATVPDLGDVWAA
                     GRRDGESSLPLTMLDESLLLLQETRATWNVQFELGADHYLDEARLRQAVLSCCQRHPL
                     VRARLTRSPQGETSYRWDFADEVDLDPLRVVECPDSVALDRLRTELYTPAIALDTSPG
                     FRVVLAQRPSGDLVLLAASHILADGVGALRLLQTIARAYQGEPDPPDPVPLAQARDLN
                     SFQPPTTRSEKWARYLEGLRRIREALDPPSRIAVVGGTDRDDFGFVFRTLDLGETTTP
                     GLMQRPAGTTVNDVLLAALHVTVGVWNFKHGAPTGRIGVQMPVNVRPPDRSWDVVSNL
                     TSMVSVSTVPDDRVDLTTAIAAVAEQTCEIRRNNRAFGLHDVLDVTKKAPLVIKRAVP
                     KLIQLTGDRFVDTAMLSNLGRIPEPPTLNKRDDSAPPELWFSPPCDPACSVAIGVVTV
                     EQRLTLVTRYRYDQFDADAAEVFTDLLIDQVTVPGPKRQS"
BASE COUNT          441 a          843 c          771 g          445 t
ORIGIN      
        1 acagtgcgtg caaccggtta acggtgtggg tccgctcctc ggcgagatca tctcgacggt
       61 ccgacagcaa ccgcagaccc acactgtgac cctcggcgcg caccaggcga agccgccggt
      121 ggtgcaccgc gactgcggcc accgaggccg cgtccagcgc gtcggtcttg cgagcatgac
      181 cggagttgag cagccgcgcc cgcgcagcca actgagccgg cacatccacc acctgctcac
      241 ccgcgccggc caacccctga gccacacccc gacccaagcc ctccgcgccc tctatcgccc
      301 agcgacgctg cggccacctg gccgcccact gccgtaactg gcgcaccgtg gtcgcggtga
      361 ccgcgacccg tatcgtggcc actggttccc gggtggactc gaccaccacc gcggtcagcg
      421 agctcttgtg cgggtcaatc ccgattgtga tcatgcacag ccttccgtcg acagcgtggt
      481 gatcccgcga cgggcaccct gacttcaggt cagtccatgc ctctgtcgag ccacgtcacg
      541 ggcggacgac ggccagcagc acgctatggg aagagccagc ccaaaaaggc ggcaaggagc
      601 atccgagcca accgaccatc gccctcggca cgctatgggc tgcagaccca gcggcctgat
      661 ccacatccaa caagtcaggg gcatcagtgc cggccagcct gccatcagcg ggtcaggcat
      721 ccattagcgg actggttcag cctgttcccc gagccttgaa catcattcgc agcttgcggt
      781 gcccgacagg cttaggactc ctgcccgcca gtgtgatgcg tgacacgcct cactcttccg
      841 gtgatgactc gatccgcgac accaccggct gcgaacttct ccacgccaga cgcgtggtca
      901 tggccaagga gcgtcattga ccaccggaat taagaccgca aaggccacca atcaaatgat
      961 ctatgatgtg gggacgaggc ccgttgccgc gcacgcacct accagcaccg cggctaccgt
     1021 gccggacctg ggggacgtct gggccgctgg gcgtcgcgat ggcgagtcgt cgctgccgtt
     1081 gaccatgctg gatgaatcgc tgttgctgct gcaggagacc cgcgcaacgt ggaacgttca
     1141 attcgagctg ggcgctgacc attacctgga cgaggcaagg ctgcggcagg cggtgctgtc
     1201 gtgctgccaa cgtcacccgc tggttcgcgc tcgactcaca cggtcaccgc agggtgagac
     1261 ctcatatcgg tgggactttg ccgatgaggt ggatctggat cccctgcggg tggtggagtg
     1321 ccctgacagt gtcgcgctgg atcggctgcg gaccgagctg tacacccccg ctatcgcgct
     1381 ggacacttcc ccgggctttc gcgttgtgct ggcccagcgg cctagcgggg atctcgtgct
     1441 tctggcggcg agtcacatcc tcgccgatgg ggtcggtgcc ctgcgcctgc tgcagacgat
     1501 tgcccgggcg tatcagggtg agccagatcc gccggatccg gtaccgcttg cccaggctcg
     1561 cgacctcaac tcgttccagc cgcccacgac ccgaagcgag aagtgggcgc ggtacttgga
     1621 agggctgcgg cgaattcggg aggcgcttga tccgccaagc cggatcgcgg tggtcggcgg
     1681 tactgaccgc gacgacttcg gattcgtctt tcgtaccctt gacctcggcg agaccactac
     1741 gccggggctc atgcagcggc ctgctggcac gacggtcaac gacgtcttgc tcgccgcgct
     1801 gcacgtcacc gtgggggtat ggaacttcaa gcatggcgct ccaactggtc ggattggggt
     1861 ccagatgccg gtcaatgttc gcccccctga ccgctcgtgg gacgtcgtca gcaacctcac
     1921 gtcgatggtc agcgtttcca ccgtgccaga tgatcgagta gacctgacaa ccgcgattgc
     1981 tgccgtggcc gagcagacct gtgagatccg acggaacaac cgtgcgtttg gcttgcatga
     2041 cgttctcgac gtcaccaaga aagcgccgtt ggttatcaag cgcgcggtgc caaagctgat
     2101 acagctgacc ggtgatcggt tcgtcgacac cgcgatgctg tcgaatctgg gccgcatccc
     2161 tgagccaccc acgctgaaca agagggatga ctctgcgcca cccgagctat ggttttcccc
     2221 gccttgcgac cccgcctgca gcgtcgccat cggagtcgtc accgtcgaac agcgactcac
     2281 actggtcacc cggtaccgct acgaccagtt cgatgccgat gccgcagaag tattcaccga
     2341 cctcctgatt gaccaggtca cggtgccagg tccgaagcgt caatcttaac ccggtcaagc
     2401 agacactgtc ggcatctttg ttgacagagt tcgatcggag ttcgtggtgg acccttcccg
     2461 tcgtggtcca agggcggtga gaggtggcca ggcttttgcc
//
LOCUS       DATADN010000204         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_475358, whole genome shotgun sequence.
ACCESSION   DATADN010000204 DATADN010000000
VERSION     DATADN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475358"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VHH53_02705"
     CDS             complement(<1..706)
                     /locus_tag="VHH53_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX2289111.1"
                     /translation="MTEGRVERHAGRSIRVAIVGVGNCAASLIQGVHYYRDADPTTRV
                     PGLMHVQFGEYHVGDLQFVAAFDVDAKKVGRDLSEAIVASENNTISICDVPPLGVTVA
                     RGHTMDGLGEHYQDIITESDETPVDVVDVLRESGADVLVSYLPVGSEDADRFYARCAL
                     DAGVAFVNALPVFIASDPEWAAKFASAGVPIIGDDIKSQVGATITHRVLAKLFEDRGV
                     ELVRTYQLNFGGNMDFL"
     gene            complement(699..1295)
                     /locus_tag="VHH53_02710"
     CDS             complement(699..1295)
                     /locus_tag="VHH53_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2289112.1"
                     /translation="MLELAVLGVLHDDPMHGYELRKRLTGLLGAFRACSFGSLYPTLR
                     RLQRRGFIVEDDERPEDSRDLRAGRGKTWSRRARRVYKLTADGKEYFATLLADAGPQT
                     WEDGCFGVHMAFFSRTPAEVRMRILEGRRRRVEERREGLRAALARAGDQIDRYTLELH
                     RMGLDTSEREVRWLNELIAHEQANPEHYTMKPERSDHD"
     gene            1427..>1625
                     /locus_tag="VHH53_02715"
     CDS             1427..>1625
                     /locus_tag="VHH53_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005442439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEX2289113.1"
                     /translation="MRTQRQVVDYALQRRALLAEVYAGRVGVMDVCDATPYLLRAAKF
                     HGEPSSVTCPVCRKETLTLVSW"
BASE COUNT          280 a          535 c          522 g          288 t
ORIGIN      
        1 tcagaaagtc catgttgccg ccgaagttga gctggtaggt gcgcaccaac tccacgccac
       61 ggtcttcgaa cagcttggcc agcacccggt gcgtgatggt ggccccgacc tgagatttga
      121 tgtcgtcgcc gatgatcggc acccccgctg aggcgaactt ggccgcccac tccggatcgg
      181 aggcgatgaa caccggaagc gcattgacga aggcaacccc cgcgtccagc gcgcaccggg
      241 cgtagaaccg gtcggcgtcc tccgagccca ccggcaggta cgacaccagc acgtcggcac
      301 cggactcgcg cagcacgtcg accacgtcga ccggtgtctc gtcggactcg gtgatgatgt
      361 cctggtaatg ctcgcccagg ccgtccatcg tgtgcccccg cgccacggtg acgcctaacg
      421 gaggcacgtc gcagatcgag atggtgttgt tctcgctggc cacgatggct tcggacaggt
      481 cgcggccgac cttcttggca tcgacgtcaa acgcggcgac gaactgcagg tcaccgacgt
      541 ggtactcgcc gaactggacg tgcatcaggc caggtacccg ggtagtggga tcggcgtcgc
      601 ggtagtagtg cacaccctgt atcagggaag cagcgcagtt gcccacgccc acaatggcta
      661 cccggatact gcgaccggcg tgacgctcga cgcgcccttc agtcatggtc cgacctttct
      721 ggtttcatcg tgtagtgctc cgggttcgcc tgctcatggg cgatcagttc gttgagccag
      781 cgcacctcgc gctcactggt gtccaggccc atgcgatgca attcgagggt gtagcggtcg
      841 atctggtcgc cagcgcgagc cagggcagcc cgcagcccct ctcgacgttc ctcgacccgg
      901 cgacgcctgc cctccaggat ccgcatcctg acttcggccg gtgtccgcga gaagaacgcc
      961 atatgcacgc cgaagcagcc gtcttcccag gtctgcgggc cggcatcggc cagcaaggtg
     1021 gcgaagtact ccttgccgtc cgcggtgagc ttgtacaccc gccgggcgcg ccgtgaccag
     1081 gtcttgcccc ggccggcgcg taggtcccgc gagtcctctg ggcgctcgtc gtcttcgacg
     1141 atgaagccgc gacgttgcaa ccggcgcaat gtggggtaca gggaaccgaa cgagcaggcc
     1201 cgaaacgccc cgagcaggcc ggtgagccgt ttgcgcaatt catagccgtg catcggatcg
     1261 tcgtggagca ccccgagcac cgctagctcc agcatacggc acctcctgac agggcactcc
     1321 gatgtatgta gctattatat cgagccgata ctttatcggc aggtgcggac tgcgttggtg
     1381 tctccaccgg aacgatgatc acgaaacggt tgcgtaggct gttggcgtgc gaacccaacg
     1441 gcaggtggtg gattacgcgt tgcagcggcg tgcgctgctc gcggaggtct acgccggccg
     1501 ggtaggggtc atggacgtct gtgacgcgac cccgtacctg ctacgcgcgg ccaagttcca
     1561 cggcgagccc agctcggtca cctgcccggt atgccgtaag gaaaccctga cgctggtctc
     1621 atggg
//
LOCUS       DATADN010000205         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1225184, whole genome shotgun sequence.
ACCESSION   DATADN010000205 DATADN010000000
VERSION     DATADN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225184"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..906
                     /locus_tag="VHH53_02720"
     CDS             76..906
                     /locus_tag="VHH53_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX2289114.1"
                     /translation="MVQDAVLPVGDGTPGRFTVRLDNFEGPFDLLLQLISQQQMDVTE
                     VALHRVTDEFIAHIRLLGDSFDLDETTEFLVVAATLLDLKAARLLPSAQVEDEEDLAL
                     LEARDLLFARLLQYQAYKQVAALFAELESAALRRYPRSVALDERFADLLPEVLLGVDA
                     AHFAETAAAVFRSKPPPPTVSLEHLHAPRISVHEQATLLRSRLARDGQASFRSLVADC
                     VHTAEVVARFLALLDLYRAAVVVFDQIDPLGDLHIRWTGGSEQPAEAALDSDEDEDYR
                     "
     gene            903..1520
                     /gene="scpB"
                     /locus_tag="VHH53_02725"
     CDS             903..1520
                     /gene="scpB"
                     /locus_tag="VHH53_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX2289115.1"
                     /translation="MSESAPIVIRDGADVSGSGDLPRLSSDGELDAALEALLLVVDSP
                     VGEDVLASAVDQPVQRVVEALQRLHDGYAAAGRGIDLRHVAQGWRLYTRDVYAGFVER
                     LLLDGQRAKLTRAALETLAVVAYRQPITRSRIAAVRGVNVDGVMRTLLARGLVSEIGA
                     DPETHGTLYCTTELFLERLGLSSLDDLPPIAPLLPDIDSIDDVPS"
     gene            1507..2256
                     /locus_tag="VHH53_02730"
     CDS             1507..2256
                     /locus_tag="VHH53_02730"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX2289116.1"
                     /translation="MSRPEVESQSLPRLHKVLAQAGVASRRAAEEMIARGRVQVDGAV
                     VREMGVRVDPEQAIIHVDGMRLELRSDVEHLALNKPRGVHSTMSDDRGRPCVGDYVAD
                     RAQRLFHVGRLDADTEGLLLLTNDGELAHRLMHPSYRVLKTYLAEVPGPVSRAVGRTL
                     RAGVDFDDGVVAVDGFRVVQSLPGKALVEMVLHEGRKHVVRRLLAEVGHPVQRLVRTA
                     IADVRLGEQRPGTVRVLSRPEVGALNRAVGR"
BASE COUNT          330 a          696 c          821 g          420 t
ORIGIN      
        1 gccatgagac ctgcttgcag ggtcgaatca tcggccatga gacctgcttg cagggtcgaa
       61 tcatcaactc cgacggtggt acaggatgcg gtgttgccgg tgggtgacgg cacgcccggt
      121 cgcttcacgg tgcggctgga caacttcgag gggccgttcg acctgctgct gcagctcatc
      181 tcgcagcagc agatggacgt caccgaggtc gctctgcacc gggtgaccga cgaattcatc
      241 gcgcacatcc gcttgcttgg tgactctttc gatctggatg agaccaccga gttcctggtc
      301 gtcgcggcga cgttgcttga tctcaaggcg gcgaggttgc tgccgtcggc acaagtcgag
      361 gacgaggagg acctcgcgct gctggaggcg cgcgacctgc tgttcgcgcg gttgctgcag
      421 taccaggcgt acaagcaggt ggcggcgctg ttcgccgagt tggagtccgc agcgctgcgc
      481 cgttacccgc gatcggtggc gctcgacgaa cgattcgccg atctgctgcc cgaggtactg
      541 ctcggcgtcg atgcggcgca tttcgccgag accgcggccg cggtgtttcg gtcaaagccg
      601 ccaccgccca cggtgtcgct ggagcatctg catgcccccc ggatatcggt gcatgagcag
      661 gcgaccctac tgcgtagccg gctggcccgc gacggccagg cgagtttccg cagcctggtc
      721 gcggactgcg tgcacactgc agaggtggtc gcccgcttcc tggcgttgct ggatctgtac
      781 cgtgccgcgg tggtggtgtt cgaccagatc gacccactgg gtgacttgca catccggtgg
      841 acgggtggct ccgagcagcc cgccgaggca gcgttagatt cagacgagga tgaggattac
      901 cggtgagcga atccgcaccc atcgtgatcc gcgacggcgc tgatgtatcg gggtccgggg
      961 acctgccccg gctttccagc gacggcgagc tcgacgccgc cctggaagca ttgctgctgg
     1021 tggtcgactc cccggtgggt gaagacgtgt tggccagcgc agtggaccag ccggtacagc
     1081 gggtggtcga agcgttgcaa cggttgcacg acggctatgc tgccgccggc cgcggtatcg
     1141 atttgcgcca cgtggcgcag ggatggcggt tgtacacccg cgatgtgtat gcaggtttcg
     1201 tggaacggct gctgctggat ggccagcgtg ccaagctcac ccgggccgct ctggagaccc
     1261 tcgccgtggt ggcctaccgc cagccgatca ctcgttcgag gattgccgcg gtgcgcgggg
     1321 tgaatgtcga cggggtgatg cgcaccttgc tggcccgcgg tctggtatcg gagatcggag
     1381 ctgatccgga gacccacggc acgctgtact gcacgacgga actcttcctt gagcggctcg
     1441 ggttgtcctc gttggacgac cttcccccga tcgcgccgct gctgcccgac atcgacagta
     1501 tcgacgatgt cccgtcctga ggtggagtcg cagtcgctgc caaggctgca caaggtgctc
     1561 gcccaggcgg gggtggcctc ccggcgtgct gccgaggaga tgatcgcccg gggccgggtg
     1621 caggtggatg gggccgtggt gcgcgagatg ggggtgcgag tcgaccccga gcaggcgatc
     1681 atccatgtcg atggcatgcg cctggagcta cgctccgacg tggaacacct ggcgttgaac
     1741 aagccccgtg gcgtgcactc gacgatgtcc gatgaccgcg gtcggccgtg tgtgggcgat
     1801 tacgtggccg accgggcaca gcgcctgttc catgtcggac gtctcgacgc cgacactgag
     1861 ggtctgctgc tgctcactaa tgacggagag cttgcccacc ggctgatgca cccctcctac
     1921 cgggtactca agacgtatct ggcggaggtg cccggtccgg tgtctcgagc agtgggacgg
     1981 accttgcgcg ccggagtgga tttcgacgac ggcgtggtgg cggtggacgg attccgggtc
     2041 gtgcagtcgc tgccgggtaa ggcactggtc gagatggtgc tgcacgaggg gcgcaagcat
     2101 gtcgtgcgcc ggttgctggc tgaggtcggc catccggtgc agcgcctggt gcgcaccgcg
     2161 atcgctgacg tgcgcctcgg cgagcagcgg cctgggacgg ttcgggtgct gtctcgcccc
     2221 gaggtcgggg ccctcaaccg cgcggtcggc cggtagcgct ggcgccg
//
LOCUS       DATADN010000206         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_928891, whole genome shotgun sequence.
ACCESSION   DATADN010000206 DATADN010000000
VERSION     DATADN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928891"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VHH53_02735"
     CDS             <1..192
                     /locus_tag="VHH53_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289117.1"
                     /translation="IQRDGEEGTSAAVAYDSRRLAGTDVVRDGVDPCSPSDHNGSLWR
                     RRYGDVMTAVGKLLRALLH"
     gene            complement(652..1194)
                     /locus_tag="VHH53_02740"
     CDS             complement(652..1194)
                     /locus_tag="VHH53_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX2289118.1"
                     /translation="MQVLTFGHSTAAADKIARLLRGAEVRRVVDVRTAPTSRHNPDAT
                     RDAMSRWLPELGISYQWDKRLGGWRKAPADTHDTALRNQSFCGYAGHMRSPEFLAAID
                     DLLASARGERTTVMCAESVWWRCHRKLIADFLMLACGVDVRHLMHDGTLRPHPLSPEA
                     RLLPERRVLVYDAGQPPLVP"
     gene            complement(1735..>2403)
                     /locus_tag="VHH53_02745"
     CDS             complement(1735..>2403)
                     /locus_tag="VHH53_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX2289119.1"
                     /translation="TELLVHTCHRRGAHAIGGMAAFIPSKDPEVNEKTLANVREDKQR
                     EAVDGFDGSWVAHPATVGLCREEFDAVLGDRPNQLDRLREDVSVGATELLDVASTPGE
                     CTEAGLRNDVSVGLHYLASWLGGQGAVAVFDLMEDAATAEIARSQVWQWLHNDVTLAD
                     GMVVTRELVDRIVDEELAKIREEAGEAFDAERYSQARSLFTEVALADELVEFLTLSAY
                     ERMP"
BASE COUNT          416 a          801 c          755 g          431 t
ORIGIN      
        1 atccagcgtg acggggagga ggggaccagc gccgctgtgg cctacgatag ccgccgcctt
       61 gcgggtaccg atgtcgtccg cgacggcgtc gatccatgct cgccatccga ccacaacggc
      121 agtctctggc ggcgccgata cggcgacgtg atgaccgcgg tggggaagct cttgcgggca
      181 ctgttgcatt aagcgatcgg cccaccgaac tggtccagct gctggcgtag atgcgggcgt
      241 cagcagcctt gaatgtctgg ttgttacggt tatccaagtt ccttgaagag ccttcgttca
      301 cgctgcgggg catggaaagg acgcccttcg ccttaatgca acagtgcccg cccgggtcac
      361 cgcccaacct tcgtctccga cgtcgcgatc aggtcggtgg actgatccac tgcccaggtc
      421 gcgttgacgt ggctgacccg ttcggcaccc acaatctcct acaggattcg ggagttgtgc
      481 tcgtcgaagc gagtacgaat atgttctttg gtatagatgt tctcttctcc gaaggagcct
      541 cgaagcgggt cgcgaatcga cgacacggtg acggaggacc gcagcatgca cgtaggccgg
      601 gaggcttccc catcatgtcg tgtatttcga tctcgaccgt gcgtgtgaag ctcagggcac
      661 caggggtggc tggccggcgt cgtagacgag gacgcgacgc tcaggaagta gacgggcctc
      721 agggctgagc gggtggggtc gcagcgtgcc atcgtgcatg aggtgtcgca catcgacacc
      781 gcaggccaac atgaggaagt cggcgatcaa tttgcgatgg catcgccacc ataccgactc
      841 ggcgcacatc accgtggtcc gttcccccct tgcgctggct agcagatcgt cgatcgcggc
      901 aaggaattct ggtgagcgca tgtgtcccgc gtagccgcaa aaggactgat tgcgcagtgc
      961 agtgtcatgg gtgtctgccg gggccttacg ccagccgccg agccgtttgt cccactgata
     1021 gctgataccg agctccggta gccatcgcga catcgcgtcg cgggtggcgt cgggattgtg
     1081 tctgctcgtc ggtgcggttc gcacatccac cacccgtcgc acctctgccc cccgcagcag
     1141 ccgagcaatc ttgtcggctg ctgcggtgct atggccgaac gtcagcacct gcatatcaac
     1201 accatgccga gggctgcggg tggcatccta gcgcctgtcc gggcgacctc cgcacaagga
     1261 acacacgacc gacctgccac ggcaatgcga gacgtcagga tcgccaagac gggcaggaca
     1321 gtcgaacgaa ccgcgccatc gctcgtcacg tcactccgtc agcacgtcgc gcagtccgag
     1381 gcgtgtggtt agtgtgcaag tccctcctcg gacacatttt cgtggcacgc ctgccacatc
     1441 tgacgtacca gtgctcaagg gcactccaac acgctgaatc agcgtcgtca tcgcaccccg
     1501 attgcttctg gtgggggcgt tcgtcatgac cggcaccgtg atcacaccga tggtcatccc
     1561 ggccacaccc ccgcggcacg gcggacaatc cgccaaatca cggtccgggg cgtcggcggc
     1621 ccgtcgcagc ggtgaactca gcgaccagcg gggccgatcg aatcgagcgg cgcgtggtcg
     1681 gtgattctgt cggtcgaact aggcacgctg tccttcgtcg gcgctgggga gccgttacgg
     1741 catccgttcg taagcggaca gggtgaggaa ctcgaccagc tcatcggcca atgccacctc
     1801 ggtgaacagc gaacgggcct gggagtaccg ctcggcgtcg aaggcctccc ccgcctcctc
     1861 ccggatcttg gcaagctcct cgtcgacgat cctgtccacc agctcgcggg tcacgaccat
     1921 cccgtcggcg agtgtcacgt cgttgtgcaa ccactgccac acctgcgagc gggcgatctc
     1981 cgcggtggcc gcatcctcca tcaggtcgaa cacggcgacc gcaccttgcc cgccaagcca
     2041 ggacgccagg taatgcaagc cgacgctgac gtcgttgcgc agccccgcct cggtgcactc
     2101 gccgggggtg gaagccacat cgagcagctc agtggcaccc accgacacgt cctcccgcag
     2161 ccggtcgagc tggttcggcc ggtcccccag caccgcgtcg aactcctccc ggcacaggcc
     2221 gacggtggcc ggatgcgcca cccaggaccc gtcgaacccg tcaacggcct cccgttgctt
     2281 gtcctcccga acgttggcca gcgtcttttc gttcacctcc gggtccttgc tggggatgaa
     2341 cgcagccatg ccgccaatgg cgtgtgcgcc gcgccggtgg caggtgtgga ccaacagctc
     2401 ggt
//
LOCUS       DATADN010000207         3735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_173636, whole genome shotgun sequence.
ACCESSION   DATADN010000207 DATADN010000000
VERSION     DATADN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173636"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VHH53_02750"
     CDS             <1..673
                     /locus_tag="VHH53_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2289120.1"
                     /translation="VVDDEPGVRRALERGLRAEGMQVITATDGPSGLRAALTGSFDAV
                     LLDIMLPGLSGYRVLERLRAAGVATPVLLVSAKDGEIDQADGLDLGADGYLVKPFSFV
                     VLVAQLRAVLRRHADQRRETVVTLGDLVIDPARRRVTWAGQPVALSPREFAVLAALAR
                     RAGSVMSKDELLHAVWGDEHAASRNAVEVYVGYLRRKLDGVGAGAVLQTVRGHGYAVG
                     AQDRP"
     gene            670..2043
                     /locus_tag="VHH53_02755"
     CDS             670..2043
                     /locus_tag="VHH53_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2289121.1"
                     /translation="MNRIHRIWAARPLPLRVAAVTTVAGLVVFLVLARASTAVVARAL
                     THTLDGKLVAAVSTAAPAVAAGRLVAQPLDAAVRIRVLDRAGTPVDGAEPTPLSAPDI
                     RRLLAGQTAWTFTSSRPGDPTRWSGRVVSTPDGTPRLVVAGAESVAHATLIGRTAGVL
                     SVAAVLAALAMGAAGWVATRFALRPVARMRVAAQELPAGERLPLPVARDELRTLAEAL
                     NNLLDRRDAATERLRRFTGDAAHELRSPVAAIRAQAEVAVTHPDPELAGATLEEVAAE
                     AQRLSVLLDDLLALAKADAGQRLPAEPVDLAEMVRDAARRAGSGKALRVTAPAPAMLH
                     AAPSDVARVLDNLLANAVRHAEHTVRVAVLPGIDEIRVLVDDDGTGVPVEHRERVFDR
                     FHRVDDGRDRGTGGTGLGLALVAETVRHYGGTARVGQAPDGGARFELNWPVGRPSSAA
                     NAAQDSH"
     gene            2313..2576
                     /locus_tag="VHH53_02760"
     CDS             2313..2576
                     /locus_tag="VHH53_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2289122.1"
                     /translation="MVRECREELAVEVVPGHQLGPDVPLPGGYLLRVYAARLADPTAQ
                     PALMEHAALRWVDVTELAELDWLDADRTVLPYLEPLLPGASPR"
     gene            2737..>3735
                     /locus_tag="VHH53_02765"
     CDS             2737..>3735
                     /locus_tag="VHH53_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2289123.1"
                     /translation="MPIAVPTRVRTDLRNVAIIAHVDHGKTTLVDAMLRQSGAVTGRS
                     AVDRVLDSGDLEREKGITILAKNTAVTRSTPDGPITINVVDTPGHADFGGEVERGLSM
                     VDGVLLLVDASEGPLPQTRFVLRKALAAHLPVVLVVNKVDRPDARIAEVVDETHDLLL
                     DLAADLEGLEAAAAESVLDMPVLYASARAGRASLNPPRDGELPDAQDLTPLFDTVLAH
                     VPPPAGDADAPLQAHVTNLDASTFLGRIALCRVRAGRLHKGQTVAWCREDGSIRPVRI
                     TELLRTEGLNRVPAASAKAGDLIAIAGIAEITIGDTLADVDDPRPLPRITVDEPVIA"
BASE COUNT          540 a         1245 c         1397 g          553 t
ORIGIN      
        1 ggtagtcgat gacgagcctg gagttcggcg tgcgctggag cgggggctgc gcgctgaagg
       61 gatgcaggtc atcaccgcaa ccgacgggcc cagcggcttg cgagcggcgt tgaccggcag
      121 cttcgacgcg gtcctgctgg acatcatgtt gccgggcctg tccgggtacc gggtgctcga
      181 gcggctgcgg gcagccgggg tggccacgcc cgtcctgctc gtctcggcca aggacggcga
      241 gatcgaccag gccgacggcc tggacctcgg tgccgatggc tacctggtca aaccgttctc
      301 gttcgtcgtg ctggtcgcgc agctgcgcgc ggtgttgcgc cggcacgccg atcagcgccg
      361 ggagacggtg gtcacgctgg gtgatctggt gatcgatccg gcgcggcggc gagtgacctg
      421 ggccgggcag ccggtggcgc tgtcgccgag agagttcgcg gtactcgcgg cgctggctcg
      481 ccgggccggc agtgtgatga gcaaggacga gctacttcac gcggtatggg gtgatgagca
      541 cgcggccagc cgcaacgccg tggaggtcta cgtcggttac ctgcgtcgca agctggatgg
      601 ggtcggagcc ggcgctgtcc tgcagacggt gcgagggcac ggttacgcgg tgggtgcaca
      661 ggaccgaccg tgaaccggat acaccggata tgggcggctc gcccgttacc gctgcgcgtc
      721 gccgcagtca ccacggtggc gggcctggtg gtgtttctgg tgctggccag ggcatccact
      781 gcggtggtgg cgcgggcgtt gacccacacc ctggacggca agctggtggc tgcggtaagc
      841 accgccgcgc ccgctgtggc tgccgggcgc ctggtggcgc agccgctgga cgcggcggtc
      901 cggatccggg tgttagaccg ggccggaaca cccgtcgacg gcgcagagcc gacaccgctg
      961 tccgcaccgg acattcgccg gctgctggcc gggcaaaccg catggacctt cacgtctagc
     1021 cggcctggtg acccgacccg ctggtccggg cgggtagtca gcacgccaga cggcactccc
     1081 cggctggtcg tggccggcgc tgagtcggtg gcgcacgcga ctctgatcgg gcgaaccgcc
     1141 ggggtcctgt cggtggccgc ggtgctggct gcgctggcga tgggggcggc tggctgggtg
     1201 gccacccggt tcgcgttgcg cccggtagcc cggatgcggg tggcagcgca ggaactgccg
     1261 gcaggggaaa ggttgccact gccggtggcg cgcgatgagc tacggacgct ggccgaagcg
     1321 ctcaacaacc tgttggaccg ccgggacgca gcgaccgagc ggctgcgacg gttcaccggg
     1381 gacgccgcgc acgaactccg ctcgccggtt gcggcgatcc gcgcgcaggc cgaggtggcg
     1441 gtgacccacc cggacccaga gctcgctgga gcaacgctgg aggaggtcgc ggccgaggcg
     1501 cagcggctgt ctgtgctgct ggacgacctg ctcgcgctgg cgaaggccga cgcggggcag
     1561 cggttgcccg ctgagccggt tgacctcgcc gagatggtcc gcgacgcggc ccgtcgcgcc
     1621 gggtcgggca aggcgctgcg ggtgaccgcg cccgcgccgg ccatgttgca cgccgcgccc
     1681 agcgacgttg cacgcgtgct cgacaatctg ctggccaacg cggtgcgcca cgctgagcac
     1741 accgtgcgag tagcggtact gccgggcatc gacgagatac gcgtgctggt cgacgacgat
     1801 ggcacagggg tgcccgtcga gcaccgggag cgggtgttcg accgtttcca ccgggtcgac
     1861 gatggccggg accgtggcac cggcgggact ggactcgggc tggcactggt cgccgagacg
     1921 gtgcgccact acggcggcac ggcccgggtc gggcaagcac cggacggagg cgcccgcttc
     1981 gagctgaact ggccagtcgg ccgcccttca agtgctgcca acgcggctca ggacagccac
     2041 taaccgctac caacccggac aagcggtggg cgtgagaagc tgtggcgcgg tgcgaggatg
     2101 tcgggcgtgc ctgagccacg tagcactgcc ctggccaaag gttcgcaacc tgagctagcc
     2161 gaccacgccc cggccgagcg gctggtggtg gccgccgccg tcctgcaccg ggatcgggtg
     2221 ctcgcccagc agcgggcata cccgcccgcg accgcgggtc gctgggagct gccgagtggg
     2281 cgcgtcgagc gtgccgaagc gccagggccg cgttggtccg ggaatgccgg gaggagctcg
     2341 ccgtcgaggt cgtgccgggt caccagctcg gcccggacgt gccgctaccc ggcggttacc
     2401 tgctgcgggt gtacgccgcg cggctggccg acccgaccgc gcagcctgcc ctcatggagc
     2461 acgccgcgct gagatgggtg gatgtcaccg agctggccga gctggattgg ctggacgccg
     2521 accgcaccgt cctgccctat ctcgaaccac tcctgccggg agcctcgccg cgataagtgt
     2581 tcatttgaca gtcggtaccg gcaccacagc tacgacaagc atccacgtgg cccttgggtc
     2641 aggtgaagca ggacgctgct gggtcaggtg aagcaggacg ctgcggcgcg gcccggtaat
     2701 gttgagggtt cccggcatcc atcgaggagt tcccgcgtgc ccatcgctgt ccccacccgc
     2761 gtgcgtaccg acctgcgcaa cgtcgccatt attgcgcatg tcgatcatgg taagacgacg
     2821 ctcgtcgacg ccatgctgcg ccaatccggc gcggtcactg ggcgcagcgc ggtggaccgg
     2881 gtgctggatt cgggcgacct ggagcgggaa aagggcatca cgatcctggc caagaatacg
     2941 gcggtcaccc gatccacacc ggacggtccg atcaccatca acgtggttga cacccctggg
     3001 catgccgatt tcggcggtga agtcgagcgc gggctgtcga tggtggacgg tgtactgctg
     3061 ctcgtcgacg ccagcgaggg cccgttgccg cagacccgct tcgtgctccg taaggcgctg
     3121 gcggcgcacc tgccggtggt gctggtggtg aacaaggtgg accgcccgga tgcccggatc
     3181 gcggaggtgg tcgacgagac ccatgacctg ctgctggatc tcgccgccga cctggaaggc
     3241 ctcgaagccg ccgctgccga atcggtgctg gacatgccgg tgctctacgc cagtgcccgg
     3301 gccgggcggg caagcctgaa ccccccacgc gatggcgaac tgcccgatgc acaagacctg
     3361 accccactgt tcgacaccgt gctggcgcac gtgccgccgc ccgccggtga cgccgacgcg
     3421 ccgctgcagg cgcacgtcac caacctcgat gcctccactt tcctgggacg gatcgcgctg
     3481 tgcagggtcc gtgctggacg cctgcacaag ggccagaccg tggcgtggtg ccgcgaagac
     3541 gggtcgatcc ggccggtgcg gatcaccgaa ctgctgcgca ccgaagggct taaccgggtt
     3601 ccggcagcca gcgcgaaggc cggtgacctg atcgccatcg ccggcatcgc ggagatcacc
     3661 atcggcgata cccttgccga cgtcgacgat ccgcgcccgc tgccgcggat caccgtggac
     3721 gagccggtga tcgcg
//
LOCUS       DATADN010000208         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_623811, whole genome shotgun sequence.
ACCESSION   DATADN010000208 DATADN010000000
VERSION     DATADN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623811"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VHH53_02770"
     CDS             complement(<1..670)
                     /locus_tag="VHH53_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289124.1"
                     /translation="MFDTSSVQPAVLEDAPVEQILDSYQRLAAAVDAGAPVEMPPSPD
                     QLPVDPAPWSTWTPSGWLGVELGQAGAVPAALSDIDLIEGVIAFDRMASWAGARQAAL
                     LAEFARRRPDDHPLAANSDIPSRASEFAADEVALALRLSRTTAANRLVMAETLAAELS
                     GTLAAWEAGVIDVLKARGPGKPLVSVIVPITMLLGLDDQPAELVGYGPIPADLAREIA
                     AEGTW"
     gene            complement(710..1402)
                     /locus_tag="VHH53_02775"
     CDS             complement(710..1402)
                     /locus_tag="VHH53_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX2289125.1"
                     /translation="MTQTGQVSNAHGVIAGVDGAGERWLVALVRNGIVSWKIAPNAAA
                     VLDYTRDCAAVGIDVPIGLPELGPRACDLEARRWLGRAASSVFLTPVRAVLHSATHGE
                     AGEALRARGEAGMTIFTWRILPKIRQWEEVPLPPWVVEVHPEVSFRAMAPEVDFARKK
                     SAPGAWQRIDALSSTMDVPAALAAAPTGVGLDDALDALAAAWSAQRFAAGTAQILGGE
                     LDRRGRPMRIIV"
BASE COUNT          283 a          594 c          496 g          220 t
ORIGIN      
        1 gccaggttcc ctcggcagcg atctcgcgag ccagatccgc agggatgggt ccgtagccga
       61 ccagctctgc gggctggtcg tccaagccga gcagcatggt gatcggcacg atcaccgaca
      121 ccaacggctt gcccggcccc cgcgccttga gcacgtcgat cactccggcc tcccaggcag
      181 ccagggtgcc ggacagttcc gcagccagag tctcagccat caccaaccgg ttagccgcag
      241 tagtccgcga caaccgcaac gctagggcga cctcatccgc agcgaactca ctggcccggg
      301 acgggatatc cgaattggcg gctaacggat gatcatctgg tcggcggcgg gcgaactccg
      361 caagcaacgc ggcctggcgc gccccggccc acgacgccat ccgatcgaac gcgatgacac
      421 cctcaataag atcgatatcc gacaacgccg caggcacggc gccggcttga cccaactcga
      481 cacccagcca tcccgacggc gtccacgtcg accacggtgc cggatccacc ggcagctgat
      541 ctggtgatgg cggcatctcg accggggcgc ccgcgtccac agctgcggcg agccgctggt
      601 aactgtcgag gatttgttcg acaggagcat cctccagcac agcgggctga acactcgatg
      661 tatcgaacat gcgttcgata gtagcacgcc ccccggaaca cagagcgggt cacacgatga
      721 tccgcatcgg acgcccgcgt ctgtccagtt cgccgcccaa tatctgagcg gtgccggcgg
      781 cgaaccgctg cgccgaccac gcagcggcca gcgcgtcgag cgcgtcgtcc agaccgacac
      841 cggtcggtgc cgcggccagt gcggcgggaa catccatggt ggacgacaat gcgtcgatgc
      901 gctgccaggc tcccggcgcg ctcttcttcc gggcgaagtc gacctcggga gccattgccc
      961 ggaaggacac ctcggggtgc acctccacca cccagggcgg cagcggtacc tcttcccact
     1021 gccggatctt cggcaggatc cgccaggtga agatcgtcat gccagcttcc ccccgcgccc
     1081 gcagcgcctc gccggcctcc ccgtgagtgg ccgagtggag caccgcccgg accggagtga
     1141 ggaacactga ggaggccgcc cggcccagcc accggcgggc ctccaggtca caagcgcgcg
     1201 gaccgagttc cggcaggccg atggggacgt cgatgccaac ggccgcgcag tcgcgggtgt
     1261 aatccagcac tgcggccgcg ttgggagcga tcttccagga cacgattccg ttgcgcacga
     1321 gcgcaaccag ccaacgttcg cccgcgccgt caaccccggc gatcacgccg tgcgcgttgc
     1381 tcacctgacc agtctgggtc acggcgttcg gtgcctactc gtagattccc tcgacccgcc
     1441 agcggcccat cccacaggcc agcagtaccg cgaggggctc cccagcggat tgggcatcga
     1501 gcaccgcctg cagtcgcgcg cagcgtcgac ctctaccggc ttcccaccag cgttcctcga
     1561 ccagccaggg cccagcccag gtcagcaccc ggc
//
LOCUS       DATADN010000209         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1379237, whole genome shotgun sequence.
ACCESSION   DATADN010000209 DATADN010000000
VERSION     DATADN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379237"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VHH53_02780"
     CDS             complement(<1..182)
                     /locus_tag="VHH53_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289126.1"
                     /translation="MVDGPHGRAEHPADDRTPSGTDEPAEQGTLFPDSVLADPHGVTD
                     QLLGYRGSAACQIAGIT"
     gene            complement(404..877)
                     /locus_tag="VHH53_02785"
     CDS             complement(404..877)
                     /locus_tag="VHH53_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX2289127.1"
                     /translation="MSEMRVVGVRVELPANQPILLLREAKGERYLPIWIGSGEATAIA
                     LEQQGVQPARPMTHDLLRDVITALGRELKQVRITELQEGTFFAELVFDGNVRVSARPS
                     DSVALALRAGVPIHAEESVLSEAGLIIPDEQENEVEKFREFLDSVSPEDFRGADT"
     gene            complement(1084..1833)
                     /locus_tag="VHH53_02790"
     CDS             complement(1084..1833)
                     /locus_tag="VHH53_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2289128.1"
                     /translation="MGSVLGQLRADFPDVTISKIRFLESEGLVQPERTAAGYRQFSAA
                     DVQRLRYVLTAQRDHYLPLRVIKEHLDAMDRGEHESPPDSRPERLTERLTDGHRSRSP
                     RSLVGVPTQAPSDLAGPADLDAADGVRMTQEELLAAAGISRKELVELEQFGLVRPGPA
                     GFYDSDAVLVARTARAMTDFGLEPRHLRAFRASADREVGLLAQIVSPMIRQRDPDARA
                     RADEVVRELAMLSVRLHALLVKAGLPGAFGS"
BASE COUNT          295 a          654 c          585 g          312 t
ORIGIN      
        1 gtgatcccgg cgatctggca ggcagccgaa ccgcgatacc ccagcagctg atcggtcacg
       61 ccgtgcgggt cggccaggac ggagtccggg aacagggtgc cttgttcggc cggctcgtcg
      121 gttccggacg gtgtgcggtc atccgccggg tgttccgctc ggccgtgcgg tccgtcgacc
      181 acccattcct cccatggctc aagccggtgg tccgtacggg ccacttcctg tgcaacccga
      241 cggtaggctg ccgcagcgta gccggtcaac gcggcacgcg ggagagcctc aacctctact
      301 tcagctcaag gggcgggatt agcgtcgtca ggtgaccgct gctcgccagt tgcgcaccaa
      361 ctgcgcgaca gcgtcgatta ggtatccgct ccgcgactgg tgactacgtg tccgctccgc
      421 ggaagtcctc cggcgagacc gagtcgagaa actcacgaaa cttctcgacc tcgttctcct
      481 gctcatccgg gatgatcagg ccggcctccg agagtaccga ctcctcggcg tggatgggca
      541 cccctgcccg cagtgccagg gcgaccgaat cactcggccg ggcagagacc cgcacgttgc
      601 cgtcgaagac aagctcggcg aagaacgtcc cttcctgcaa ctcggtgatc cggacctgct
      661 tgagctcacg tcccagcgcg gtgatcacat ctcgaagcaa gtcgtgggtc atcggccgag
      721 ctggttgcac gccctgctgt tccagggcga tagcggtcgc ctccccagaa ccgatccaga
      781 tcggcaggta gcgctcgcct ttggcctccc gcagcagcag gatcggctga ttcgccggca
      841 attccacccg gaccccgacg acacgcatct cgctcatcgc gttgcgcctc cctcccgccg
      901 caccctgtgg gccaggtgcc ttgaggatcg tcgtgctcgg cggcggattg ggcaccggcc
      961 gcgagtagat gccagcccag tcgcgtgtcc accatcgcta cgcaagacgc tacacgcggc
     1021 acccgcccgg tgccgagtcc agcgggtgac caattcgtgc gctgtgctca ttcccgctct
     1081 tgttcagctc ccgaacgcgc caggcagccc cgccttgacg agcagggcgt gcagccgtac
     1141 cgacagcatt gccagctccc gtaccacctc gtcggcccgg gctcgggcgt ccgggtcccg
     1201 ctgccggatc atcgggctga cgatctgcgc cagcagaccc acctcccggt ccgccgacgc
     1261 ccggaaagcc cgcagatgcc gaggctccag cccgaagtca gtcattgccc gggcggtccg
     1321 ggccacgagc accgcatcgg agtcgtaaaa gccggcgggg ccgggccgga ccaggccgaa
     1381 ctgctcgagc tcaaccagct ccttcctgct gatgccagcg gccgcgagca attcctcctg
     1441 ggtcattcgc acgccgtcag cagcgtcgag gtcagctggc cccgccaggt cggacggcgc
     1501 ttgggtgggc acaccgacca gtgagcgcgg tgaccgtgac cggtgcccgt cagtgagtct
     1561 ttcggtgagt ctttctgggc ggctgtccgg gggactttcg tgctcacccc ggtccatcgc
     1621 gtccaggtgc tccttgatca ccctgagcgg cagatagtga tcgcgctgag cggtgagcac
     1681 gtaacgcagc cgttgcacgt ccgcagcaga gaactgccgg tatccggcag cagtgcgctc
     1741 cggctggacc agtccttccg actccaagaa cctgatcttg gagatggtga catccggaaa
     1801 gtcagctcgc agctggccga gcaccgagcc gatgctcgct ccgggc
//
LOCUS       DATADN010000210         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1225646, whole genome shotgun sequence.
ACCESSION   DATADN010000210 DATADN010000000
VERSION     DATADN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225646"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1515
                     /locus_tag="VHH53_02795"
     CDS             223..1515
                     /locus_tag="VHH53_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012751064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2289129.1"
                     /translation="MLDAGLLDGVMDRVDRDGLALTGVGGFLPELVKAVLERGLQAEL
                     TEHLGYERGDPAGRGSPNSRNGSTPKTLATEVGEIPLAVPRDRVGSFEPRLVPKGCRR
                     AGGLDEMIVSLYAGGMTVRDIQHHLARTLGTELSHEAISKITDSVLEEVKAWQARPLE
                     ELYPIIYLDALVVKVRDGHQVRNKAAHIAVGVDLDGIRHVLGIWVQASEGAKFWAGVC
                     AELRNRGIRDVLIVCCDGLTGFPEAIEATWPQATVQTCTVHLIRAAMRFVSYQDRKKV
                     AAALRPIYTAPTVEAAESELLAFAESDLGRRYPAAVATWENAWERFIPFLAFPPELRK
                     IIYTTNSIESLNYQLRKIIKNRGHFPNDDAVVKLLWLAIRDIEDKRARHRAAERGVAR
                     ADRRAPGRLVEGAVVQGWKQALGALALAYPDRLAGYLA"
     gene            complement(1763..1998)
                     /locus_tag="VHH53_02800"
                     /pseudo
     CDS             complement(1763..1998)
                     /locus_tag="VHH53_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895475.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          337 a          693 c          702 g          266 t
ORIGIN      
        1 ggagcgcacc cgcatggccg gtgtttgcag cagcacgcgt gagggcgatg gctgcgggcc
       61 gaacccgaga cggtggaggt gtcagatcgt ctgtgtacaa gatcgacgat cagttgatag
      121 gagtacgcag tgaccgtgac cgaagatcag cagtcagcgc acccgttggg ggtgccggcc
      181 cagccgatat cgcctgcgac ggcccgtgag gcaatcaacg agatgctcga cgccgggctt
      241 ctcgacggcg tgatggatcg agtcgaccgc gacgggctgg ccctgaccgg ggtcggcggg
      301 ttcctgccgg agctggtcaa ggcggtgctc gagcgcggcc tgcaggccga gttgaccgag
      361 cacctgggct atgagagggg cgatccggcc gggcgcggca gcccgaactc gcgcaacggc
      421 agcacgccca agacgctggc caccgaggtc ggcgagattc cgctggcggt gccgcgggac
      481 cgggtcggca gcttcgagcc gcggctggtg cccaagggct gccggcgcgc cggcggcctg
      541 gacgagatga tcgtgtcgct gtacgcgggc ggaatgacgg tgcgcgatat ccagcaccac
      601 ctggcccgca cgctgggcac cgagctgtcc cacgaggcca tctcgaagat caccgactcg
      661 gtgctcgagg aggtcaaggc ctggcaggcc cggccgttgg aggagctcta cccgatcatc
      721 tacctggacg cgctggtggt gaaggtgcgc gatggccacc aggtccgcaa caaggccgcg
      781 cacatcgccg tcggggtcga cctggacggg atccgccacg tgctggggat ctgggtgcag
      841 gcatcggagg gggcgaagtt ctgggccggg gtgtgcgccg agctgcgcaa ccgcggtatc
      901 cgcgacgtgc tgatcgtctg ctgcgacggg ctgaccggct tcccggaggc gatcgaggcc
      961 acctggccgc aggccaccgt gcagacctgc acggtgcacc tgatccgcgc ggcgatgcgg
     1021 tttgtctcct atcaggatcg caagaaggtg gccgcggcgc tgcgcccgat ctacaccgcg
     1081 cccaccgtgg aggccgccga gagcgagctg ttggccttcg ccgagtccga cctcggccgc
     1141 cgctacccgg cggccgtggc gacctgggag aacgcctggg agcggttcat cccgttcctg
     1201 gccttccccc cggaactgcg caagatcatc tacacgacca acagcatcga gtcgctgaac
     1261 taccaactcc ggaagatcat caagaaccgg gggcacttcc ccaacgacga cgccgtggtc
     1321 aagctgctgt ggctggccat ccgcgacatc gaagacaagc gcgcccggca ccgcgccgcc
     1381 gaacgcggcg tggcccgcgc cgaccgccgg gccccgggcc ggctggtcga aggcgcggtg
     1441 gtgcagggct ggaaacaggc cctcggcgcg ctcgccctgg cctaccccga ccgcctggcc
     1501 ggctacctgg cctgagccca ggaccacacg tggcccttcc gccgccccag ccgatcactt
     1561 ccaccgcgcc ggaggtgtcc acgtggagcg cccgcagcgc agcgaggacg aacgacgtgg
     1621 acaccgacaa gcggtggaag accatgaccg gcggcggaac cgggcgaccg aaacaacgcc
     1681 atcgaccacc agcgggtccg gcacagcaac cgggccctaa tccaacaagg tgatctctta
     1741 cacagcgatc ttgacaggct ctcgagccct cgaccagcgc caacggatgc ttggtgccgt
     1801 cgaggccgat cccgagggcg accacgcagc agtgctcgcc gaagtgcacc ccgtccacca
     1861 tcagcgcccc caagtccagg ccggacaggt ccgcggcgag cgagctcggc cagcgcggtc
     1921 tcggccgccg cgacgaaccg gcgcgacacc gccaacttgc tggtgccggt cgccgcctgc
     1981 tcggtgcgtg tgccgacc
//
LOCUS       DATADN010000211         4903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1079898, whole genome shotgun sequence.
ACCESSION   DATADN010000211 DATADN010000000
VERSION     DATADN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079898"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VHH53_02805"
     CDS             <1..757
                     /locus_tag="VHH53_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289130.1"
                     /translation="PYDRARFPRVTVHDNVSVQHRLVTERFGIQRLQLVTGWSMGAGQ
                     TYQWAVSYPEMVPRILPFCGSAKTSEHNIVFLEGVKAALTADGAFQEGWYEKPPTRGL
                     RAMARVYAGWGFSQAFYWQRLYHTEMGYSSLEDFLISFWEGFFLPKDANNLLAMLWTW
                     QNGDIGKTPGFDGDFERALGAITARAIVMPAEKDLYFPPEDNAYEVRHMSNAELRVIP
                     GVWGHFAGGGLDADRTDTKFIDDALKELLTQEV"
     gene            964..2421
                     /locus_tag="VHH53_02810"
     CDS             964..2421
                     /locus_tag="VHH53_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX2289131.1"
                     /translation="MTTTELAARIRRRELSPVEVVDAFIERIEARNQSLNALVYLGFD
                     DARERAREAEQALVSGAELGVLHGVPTAIKDLFDFNPGWPATFGGVRALQEYTLDISC
                     AFAERIQRAGAIVLGKTNSPVMGFRGTCDNYLFGPSRNPFAPAKNTGGSSGGSAAAVA
                     DGLLPLAEGTDGGGSIRIPAAWCGVYGYKASFGRIPYVNRPNAFSGINPFLFEGPITR
                     TVEDAALALDALAGYDSREPFSIDTGERFMPAVRRSISGWRIAYSPDFDVFPVDKRVT
                     EVVSTAVQAFEQAGAQVEEVRLGINRDQRELSDLWCRLILPINLETFENFKRNGIDLL
                     ADHRDDFPPEYLRWIDEGYRMSALDLSRDQQTRSEVYDAIQRVLDDYHLLVTPTLACL
                     PVDNADDGNTLGPSRINGVEVDPLIGWCLTYFVNFSGHPAASIPAGLAEGQLPVGMQL
                     IGRRYGDADVLAASATFEQIRPWQDTYQLCADRTL"
     gene            3118..3399
                     /locus_tag="VHH53_02815"
     CDS             3118..3399
                     /locus_tag="VHH53_02815"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX2289132.1"
                     /translation="MPQDAHVPGGVVRVIELVGSSPNSFSDAVRNAVRLASQSLPNIR
                     GVEVLSSNADVDDAGNLTLYKVQCKIAFLVEPAQVGQTPGWDIATNRPT"
     gene            complement(3685..4680)
                     /locus_tag="VHH53_02820"
     CDS             complement(3685..4680)
                     /locus_tag="VHH53_02820"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX2289133.1"
                     /translation="MASSDEVLATIPVDMEPRWVAITPDGHRAYVTLSKDQPGDLPTR
                     SAVAVIDTQTNTVAATVTIDHRSNLDQPSGVVITPDGRRAYVSNFDGRGVVDVIDTAT
                     NTVVETLTVSGRGGSSTGIAITPNGRHIYVATGNELDLPNEGKVSVIDTATNTIITTI
                     RGNPRPSTVTVTPNGDFVYVLDDDGSPLVIDTATHKARLAEELEGVVTNGRIAFTPDG
                     LHLYAVSEGSDLVQVLEVATGTIVAVVDVFGGRSTDVALSRDGRHVCVTQRPGNALKR
                     PLWVIETATHKVIGSPAEWSGTANGLAITPDGSAVYVADRNSRAVRVIPVRPQIG"
BASE COUNT          816 a         1627 c         1674 g          786 t
ORIGIN      
        1 accatatgac cgcgctcgct tcccccgggt gaccgtccac gacaacgtct cggtgcagca
       61 ccggctggtg accgagcggt tcggcatcca gcggcttcag ctggtcactg ggtggtcgat
      121 gggcgccggc cagacctacc aatgggcggt cagctacccc gagatggtcc cgcggatcct
      181 gccgttctgc ggctcggcca agaccagcga gcacaacatc gtcttcctgg agggggtcaa
      241 ggcggcactc acggcggacg gggcgttcca ggaaggctgg tatgagaagc caccgaccag
      301 gggcttgcgc gctatggcac gggtgtacgc cggctggggg ttctcgcaag ccttctactg
      361 gcagcggctg taccacaccg agatgggcta ctcctcgctt gaggacttcc tcatctcctt
      421 ctgggaaggc ttcttcctgc ccaaggacgc caacaacctg ctggccatgc tgtggacctg
      481 gcagaacggg gacatcggca agacgccagg cttcgacggc gacttcgagc gagcgctcgg
      541 cgcgatcacg gccagggcga tcgtcatgcc ggcggagaag gacctctact tcccgcccga
      601 ggacaacgcc tacgaggtca ggcacatgtc aaatgcggag ctgcgggtga tccccggcgt
      661 ctgggggcac ttcgccggcg gcgggctcga cgccgaccgg accgacacca agttcatcga
      721 cgacgcgttg aaggagctcc tcacccagga ggtatgacgt gccggtgctg tgaggaaccg
      781 gaggcaaaca gcggccagat gggccacccg acgcgcggtc tggtccttgg cttgaagggg
      841 caggccgtgt ggtcgggacg gaaccttcgc cgccaccagg cgattgaccg tcgccgccgc
      901 acaaaccggc ggtgcttctg gaggaggtgg gccagaatgg cgttggcaga tgagcttgcc
      961 tacatgacga cgacggaact ggcggcacgg atccggcggc gcgaactgtc gccggtcgag
     1021 gtcgtggatg ccttcatcga gcggatcgag gctaggaacc agagcctgaa cgccctggtc
     1081 tatctcggct ttgatgacgc ccgcgagcgg gcccgggagg ccgagcaggc attggtgtcc
     1141 ggagccgagc tcggcgtact gcatggggtg ccgaccgcga tcaaggacct gttcgacttc
     1201 aaccccggct ggccggcgac cttcggcggc gtcagggccc tccaggagta caccctggac
     1261 atctcctgcg ccttcgccga acggatccaa cgcgctggcg cgatcgtgct cggcaagacc
     1321 aacagcccgg tgatgggatt ccggggcacc tgcgacaact acctgttcgg gcccagccgc
     1381 aaccccttcg cgcccgccaa gaacaccggg ggctcgtcag gaggcagcgc cgccgcggtc
     1441 gccgacggac tgctgcccct ggcagagggt accgacggcg gtgggtccat ccgcataccg
     1501 gccgcctggt gcggggtgta cggctacaag gcttcctttg gtcgcatccc ctacgtcaac
     1561 cggcccaacg ccttctctgg catcaacccg ttcctgttcg agggaccgat caccaggacg
     1621 gtcgaggacg ccgcgctcgc cttggacgcc ctggccggct acgactccag ggaaccgttc
     1681 agcatcgaca ctggcgagcg cttcatgccg gcggtgcggc ggtccatcag cggctggcgg
     1741 atcgcctaca gccctgactt cgacgtcttc ccggtcgaca aacgcgtcac tgaggtcgtg
     1801 agcaccgctg tgcaggcctt cgagcaggct ggtgcgcagg tcgaggaggt ccggctgggc
     1861 atcaaccggg accagcgcga gctgagcgac ctgtggtgcc ggctcatcct gccgatcaac
     1921 ctggagacgt ttgagaactt caagcgcaat gggatcgacc tgttggccga tcatcgggac
     1981 gacttcccgc ccgagtacct gcggtggatc gacgagggct accgcatgag cgccttggac
     2041 ctctcccgcg accagcagac ccgctcggag gtctacgacg cgatccagcg ggtcctggac
     2101 gactaccacc tgttggtcac cccgacgctg gcctgcctgc cggtggacaa cgccgacgac
     2161 ggcaacaccc tcggcccgag ccgcatcaac ggcgtggagg tcgacccgct gatcggctgg
     2221 tgcctcacct acttcgtgaa cttctccggc cacccggcgg cgtcgattcc cgccggcctg
     2281 gccgaggggc agctgcccgt tgggatgcag ctgatcggcc gccgctacgg cgatgcggac
     2341 gtgctggcgg caagcgccac ctttgagcag atcaggccct ggcaggatac ctaccagctg
     2401 tgtgccgaca ggacgctgtg atggcgacct acccagcctc aagagcccgc tcaaagtctg
     2461 atccccgatc agctgctagg cgagcgcata acttcaccgg aggagaaccg atgacttcgg
     2521 cgccgacggt cgagcgtgac ctgggataac gacgttccgc acagcggttc ctggtggttc
     2581 cagggtaagg atgggaacac ctagcgcgct ccaacctgac aggacactag cggtagcatc
     2641 ccacttggcc tagcaggacc cgtcgcggct tcctccagcg tgccggcgag cggccctcgt
     2701 ggcgggagcc ttgttctccg accacgccca gggggaggga tctggccgga cggtgctggc
     2761 atgcccggcc ctcagccgca agcgccccac caaccaagct caggctcccg agccggccga
     2821 tggccggcaa ccttgatgca tggccgacgt cgttggctcc accgcttgcc cctcggggtc
     2881 tctcggcatg ccacggtgtc caccgtcccc ggttccaagg gcagggcaga ccgcccggcc
     2941 aggcaacaga aggggacaag accatgaccg agacaagcgt cagcttcgcc ggccacctca
     3001 ccgacgaccc cgaactgcgc gagcagcacg gttgaggcga ttgccggttg gctcccctgg
     3061 gtaccagccc cgcgcctgat ctccggggcc gcacgccccc agagcaagga ggtacccatg
     3121 ccgcaggacg cccacgtgcc cggcggagtc gtcagggtca tcgagctggt ggggagctcc
     3181 cccaactcct tcagcgacgc ggtgcgcaac gccgtgcggc tggccagtca gagcctgccc
     3241 aacatccgcg gggtcgaggt gctctccagc aacgccgacg tggacgacgc cgggaacctg
     3301 accctctaca aggtccagtg caagatcgcc ttcctggtgg agcccgctca ggtgggccag
     3361 acccccggtt gggacatcgc caccaaccgg ccgacgtagg ccatgcgtgc cagcgcccgc
     3421 agggggggac agggagcggc cgcaggctta gccgcgacca tgcaagcgtg cgcccaatcg
     3481 ctgtggaagg gcgatcccgt ggtggccctg ggccggctcc agtcgcggag ctggaccgcg
     3541 gaggatggca gcgcccggtc ggtggtggag gtcgtggctg aggagctggg accaagcctg
     3601 cgctgggcga cggccccgac gaccacggct gcgaggagtc aggacgagca cccggtgggt
     3661 cggcccgctg ggatgtggag cgggttaccc gatctggggt cggaccggga tgacccgtac
     3721 cgcgcgcgag ttccgatcgg ccacgtagac ggccgacccg tccggcgtga tcgcgagccc
     3781 attggccgtg ccggaccatt cggccgggga gccgatgacc ttgtgggtcg cggtctcaat
     3841 cacccacagg gggcgcttta aggcgttgcc tggccgctgg gtcacgcaga cgtgacggcc
     3901 atcacgcgag agcgccacgt cggtcgagcg gccaccgaat acgtcgacga cggcgacgat
     3961 tgtgccggtg gcgacctcca acacctggac caagtcgctg ccctcgctga cggcgtagag
     4021 atggagccca tccggggtga acgctattcg cccattggtg accactccct ccagctcctc
     4081 ggcaagcctg gccttgtggg tcgcggtgtc gatcactagg gggctcccgt cgtcgtcaag
     4141 cacgtagacg aagtccccgt tcggcgtaac ggtcactgtg gagggacgcg gattcccgcg
     4201 gatggtggtg atgatggtgt tggtggcggt gtcgatcacg ctgaccttgc cctcgttggg
     4261 caggtcgagc tcgttcccgg tggccacata gatgtggcgg ccgttcggcg tgatcgcgat
     4321 gccggtcgag ctaccgccgc ggccgctgac ggtgagggtc tcgacgacgg tgttggtggc
     4381 ggtgtcgatc acgtccacca cacctcggcc gtcgaagttg gacacatagg cccggcgacc
     4441 atctggtgta atcaccacgc cgctgggttg gtccaggttg ctgcgatggt cgatggtgac
     4501 ggtggcggcg accgtgttgg tctgagtgtc gatcacagcg accgcgcttc gcgtcggaag
     4561 gtctccgggc tggtccttcg acaaggtcac ataggcgcgg tgcccgtccg gcgtgatcgc
     4621 gacccacctg ggttccatgt cgaccggaat cgtggccagg acctcatctg aggacgccat
     4681 cgcggccccc cttcctcgtc tggcaatgga tcgtgtcaca gcttgacgcc agcgtgttgc
     4741 cgatcttgtc cgcaacgaag cggctcatga ccacgccaga ctcgtgatcg cggtcatgga
     4801 cggccgagga tggcagcgtc cgcaggtcgt caaggtcctg gccgagcagg tcgggtcagc
     4861 cctgtggcgg gcaacggcga cgacgaccgg gatgacgcgg agc
//
LOCUS       DATADN010000212         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_929463, whole genome shotgun sequence.
ACCESSION   DATADN010000212 DATADN010000000
VERSION     DATADN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929463"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VHH53_02825"
     CDS             complement(<1..707)
                     /locus_tag="VHH53_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289134.1"
                     /translation="MSGQLAAIVLVVLVGSALALTGLTARWVVGHPERIRAGLAWVAE
                     RPAVVWVRSRYPRQWRFLDRRLAPGEATGLALTLGVGMVLALGLSFRQLLDNVLESDG
                     IAVADRPVLSFLAAHREPWLVTAMQVISDVGSPVGVGILATVVGVTVAWVRRSWLPLL
                     VAGFGTAGIGIINLTVKGLVSRGRPPAAIAVLGEDGYSFPSGHTIGTTVVWLLSAWMV
                     NRWVIRGQISRDLLWIGA"
     gene            810..1079
                     /locus_tag="VHH53_02830"
     CDS             810..1079
                     /locus_tag="VHH53_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX2289135.1"
                     /translation="MTVTLARIYDLPHALRDGQQFARHLDGRQPAVFLDYDGTLTPIV
                     DRPEDAVISDRMRDAVCGLTARATVCVVSGRDRPLVTRWMGIDGR"
     gene            1122..1726
                     /locus_tag="VHH53_02835"
                     /pseudo
     CDS             1122..1726
                     /locus_tag="VHH53_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT          286 a          609 c          575 g          256 t
ORIGIN      
        1 gccccgatcc atagcaggtc ccggctgatt tgaccgcgga tcacccaccg gttgaccatc
       61 cacgccgaca acaaccagac caccgtggtg ccgatggtgt gcccggaggg aaacgagtag
      121 ccgtcctcgc ccagcaccgc gatggccgcc ggtgggcggc ctcgactcac cagccccttc
      181 accgtcaaat tgataatacc gatgcccgcc gtcccgaacc cggcgaccag caatggcagc
      241 cacgacctgc ggacccatgc caccgtcaca cccaccacgg tggccaggat gcccactccc
      301 accgggctgc ccacatcgct gatcacctgc atcgcggtga ccaaccaggg ctcgcggtgc
      361 gcggcgagga agctcagcac cgggcggtct gccaccgcga tgccgtcgct ttccagcacg
      421 ttgtccagca actgcctgaa actcagcccc aacgccagca ccatgccgac gccgagcgtc
      481 aacgccagcc cggtggcctc tcccggcgcc aatcggcgat ccaggaaccg ccactgcctc
      541 gggtagcgcg accgcaccca gaccaccgct ggccgttcgg ccacccatgc gagcccggcc
      601 cggatccgct ccggatgccc cacaacccac cgcgcggtca ggccggtcag ggcgagcgcg
      661 ctccccacga ggaccaccaa gacgatcgcc gccagctgcc cactcaagcg ttgcccctcc
      721 tccttgctcg tgagtggcat tcagtgctgg gacactgtta cccactcacg accttgacgt
      781 acaccggcga ggccgataga cgatgactcg tgacggtgac cttggcgcgc atttacgacc
      841 tgccacacgc gctgcgcgat ggccagcagt tcgcgcggca tctggatggt cgtcagccgg
      901 cggtgttcct agactacgac ggaacgttga cgcccattgt ggaccggccc gaggatgcgg
      961 tcatctcgga caggatgcga gacgcggtgt gcgggctcac cgcgcgcgcc acggtctgcg
     1021 tggtcagcgg tcgggatcgg cccctggtca cccggtggat ggggatcgac ggccggtagc
     1081 catggcttcg acacgccttc agggctctca aaggagatcg gatgggcatc ggtgtcgccg
     1141 tcgcagatcc cagcgcgctg accacaagcc tgaccatcgg tgtggacatc ggtggtacga
     1201 aggtcgcggc tggtgtcgtc gacgagcgcg gggcgatcct cgccaccacg catctggaca
     1261 cccccgccga cgacgtgtcg cggatcgagg acgcgattgc cgccgcggtg ggtgagctcg
     1321 ccagcaagta cgacgtcgat gctgtcggca tcggtgcggc cggtttcgtc gacgcgaagc
     1381 gggggactgt gatgttcgcc ccccatctcg cctggcggga cgagccgctg caggcgtcac
     1441 tggagcaccg gctgcggctg cccgtggtgg tcgagaacga cgccaacgcc gccgcctggg
     1501 cggaggtccg cttcggtgcc ggccgcgatg cagaccatgt ggtgacaatc accgtgggga
     1561 ccgggatcgg gcggcatcgt ggtgaacggc cagctgatgc ggggccaatt cggtgcggcc
     1621 gcggagttcg gtcacctcac cgtcgtgccg ggcgggcggc gctgcgaatg cggcttgcgg
     1681 ggttgctggg aggagtacgc aagcggccgg gggctcgagc gcgagg
//
LOCUS       DATADN010000213         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_624188, whole genome shotgun sequence.
ACCESSION   DATADN010000213 DATADN010000000
VERSION     DATADN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624188"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VHH53_02840"
     CDS             complement(<1..1153)
                     /locus_tag="VHH53_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX2289136.1"
                     /translation="MPAVLVLAVFGVALMGQFTLGRLGIEQFGELDLRLVAFPALVAA
                     VALWRFQPGHRDYRHAWPPTSLWAIAMVAYLALTAFWAPYEARISENLTDLVFVGLLI
                     VVATVVSAPDPARARRVTLAAMYVAAVVYALAGVLIGETDAQGRPTAFAGGPNVYTRV
                     ILLGVIAAIALTALYGRRLFLVAIPLLASTALLPASRGAIVAALATVIVFGALTWGRW
                     SLRGIGLAVLVTAGTLIVTWNLLPSAAVDVVRYRFVDQVFERNELSGRPELISQALTI
                     FVGDPLIGAGLDSYFVRFGLGIGLEYPHNLVLDLAATGGVAALAIFAMFVVALIRDSR
                     PLRGMTADQLAMLLSACYLGAASMFSGEFYDSRFFWIFAVLAVNYSAVRC"
     gene            complement(1241..>2024)
                     /locus_tag="VHH53_02845"
     CDS             complement(1241..>2024)
                     /locus_tag="VHH53_02845"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX2289137.1"
                     /translation="TVAVVSPHDADGKTATACNLAVALAQTGRKVALVDGNIRGPGVM
                     DYLGEHGRKAESAAGESGNRQSGGLGGVLTGALPLTAAMRSVGPKGFDVLQAGRWFVG
                     LSELLSSPSMELLLDELADSYDTVIVDTAALLHGSDGATLAALCDTTIMVARHGQTKR
                     KDLQAATDLLRSMSTAMIGGVLSRSPRSGARSGVRPGRAAERVQRATVPPGAARIPPR
                     HAAADEPAGHDVWVPPGVAHGATPAPDEQTPPTLGGAGTSRP"
BASE COUNT          381 a          683 c          690 g          270 t
ORIGIN      
        1 cgcaccggac tgccgaatag ttgaccgcca gcactgcgaa gatccagaaa aaccggctgt
       61 cgtagaactc gccggagaac atgctggcgg cgccgagata gcaggcggac agcaacatcg
      121 cgagctggtc ggcggtcatc ccccgcaacg gccgggagtc gcggatcagt gccacgacga
      181 acattgcgaa gatcgcgagg gctgctactc caccagttgc cgccaggtcg agaacgaggt
      241 tgtgcgggta ctccagaccg atcccgagcc cgaaccgcac gaagtagctg tccagacccg
      301 ccccgatgag tggatcaccg acgaagatcg tcagtgcttg ggagatcagc tcgggtcggc
      361 cggacagctc gttgcgctcg aagacctgat cgacgaagcg gtaccggacg acgtcgacgg
      421 ccgccgacgg cagcagattc caggtcacga tcaacgtgcc ggcggtgacg agcaccgcga
      481 ggccgatccc gcgaagtgac cagcggcccc aggtcaacgc accgaatacg atcaccgtgg
      541 ccaacgcggc gacgatcgcc ccacgcgagg ccggaaggag cgccgtcgac gccagcagcg
      601 ggatggcgac caggaacagc cgccgaccgt acagtgccgt caaggcgatc gcagcgatca
      661 ccccgagcag gatgacacgg gtgtagacgt tgggcccgcc agcgaaggcg gtgggccgcc
      721 cctgcgcatc cgtctcgccg atgagcacgc cggccagcgc gtagacgacc gctgcgacgt
      781 acatcgcggc gagtgtcacg cggcgggcgc gcgcggggtc cggcgccgac acgacggtgg
      841 ccacgacgat cagcagcccc acaaagacga gatcggtcag gttctcgctg atccgggcct
      901 cgtaaggtgc ccagaatgcc gtcagagcaa ggtaggcgac catcgcgatg gcccacagcg
      961 atgtcggggg ccaggcgtgc cggtagtcgc ggtgcccggg ttggaaacgc cacagcgcaa
     1021 ccgcggctac gagcgctggg aacgccacga gtcgcagatc gagctcgccg aactgctcga
     1081 tgcccagacg tcccagggtg aactgaccca tgagcgcgac gccgaacacg gccagcacaa
     1141 gtacggccgg catccggaac ccagccgcca cctgtgtcga actggtcgcg atggaatgct
     1201 ccttccgagt atcggtcccg ggagggagcc ggtccgaacc tcacgggcgg gaggtgccag
     1261 ccccgccaag ggtggggggt gtttgctcgt cgggtgcagg cgtcgcccca tgcgccactc
     1321 ccgggggaac ccagacatcg tggcccgcgg gctcgtcagc ggccgcgtgc cggggtggta
     1381 tgcgggcggc tccgggcggc accgtagccc gctgaacccg ctcggccgca cgaccgggcc
     1441 gtacgccgga acgtgccccg gaacgcggtg acctgctgag gactccaccg atcatcgcgg
     1501 tggacatgga gcgcagcaga tccgtggcgg cctgcaggtc cttgcgcttc gtctggccgt
     1561 gccgggcgac catgatcgtc gtgtcgcaca atgccgccag ggtggcccca tcgctgccat
     1621 gcaggagagc ggctgtatcg acgataaccg tgtcgtagga atcagccagt tcgtcgagca
     1681 gcaactccat gctgggcgag gagagcagct cactgaggcc gacgaaccac cgcccggcct
     1741 ggagaacgtc gaaccccttg gggccgaccg accgcatcgc ggccgtcaat ggcaatgccc
     1801 cggtgaggac gccgccaaga cccccagact gacgattccc cgactcacca gcggccgatt
     1861 ctgccttgcg gccatgttcc ccgaggtagt ccatgactcc ggggccacgg atgttgccgt
     1921 ccaccagcgc gaccttccgg ccggtttggg cgagtgcgac agcgagattg cacgctgtcg
     1981 ctgttttgcc gtcagcgtcg tgcgggctca ccacggccac agtc
//
LOCUS       DATADN010000214         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1080076, whole genome shotgun sequence.
ACCESSION   DATADN010000214 DATADN010000000
VERSION     DATADN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080076"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VHH53_02850"
     CDS             <1..290
                     /locus_tag="VHH53_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289138.1"
                     /translation="RTSRLPTPPAGAGSPFAWGREEVVRELLSDAFELDIEERITTSN
                     VATGEEYWLKHLTKIGASYSIRRGLTSSTAATGRAAESHTREYLLVVGTRR"
     gene            complement(316..942)
                     /locus_tag="VHH53_02855"
     CDS             complement(316..942)
                     /locus_tag="VHH53_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2289139.1"
                     /translation="MADLIRLLLVDDHEMVRTGLRTFLELQQDMEVVGEASSGEQALA
                     LVAAMRPDIVLLDLVLPGLSGLETARRLRDTHPDVKVVVLTSFSGQDSVLPAVRAGVA
                     GYLLKDVGPAELAGALRSVHHGGASLHPRVAAAVMHSVTDRDPLTTREREVLRLIGRG
                     LSNRLIARELVLSEKTVKAHVSAILSKLAVADRTQAALLAVRDGLVDP"
     gene            complement(935..>1586)
                     /locus_tag="VHH53_02860"
     CDS             complement(935..>1586)
                     /locus_tag="VHH53_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2289140.1"
                     /translation="AVVNAQRQERLRELSIVDERTRIARDLHDSVTQTLFSLTLAAES
                     AATVAGAGSPAYAHLDRVRDLSRTALDELRALVDTLRPPDVDREGLGVALRKRVDLLR
                     AVHDVPILLEVHGREGRHTGEVGCEVLKVANEAIGNALQHASPHRIRVRLDVSVAVRL
                     TVSDDGSGFDLAVTARTSRRLGLASMRERAETLGGTLQVDTAPGAGTTVTLEVPTG"
BASE COUNT          255 a          552 c          532 g          247 t
ORIGIN      
        1 tgcggacgtc gcgcctgccg acgccgccgg caggcgccgg gagtccgttc gcctgggggc
       61 gcgaggaggt agtgcgcgaa cttctcagcg acgcgtttga actcgatatc gaagaacgca
      121 tcaccaccag caacgttgcg accggagagg aatactggct gaagcacttg acgaagatcg
      181 gcgcgagcta ttccatcaga cgtgggttga cttcttcgac agccgctacc ggtcgggcgg
      241 cggagtcgca cacccgtgag tatctcctcg tcgtaggcac gcgacgctga acggcccggt
      301 ggcacggctg ccgggtcagg gatcgaccag cccgtcgcgc accgccagca gcgctgcttg
      361 ggtgcggtcg gccactgcga gcttgctcaa gatggcgctg acgtgtgcct tcactgtttt
      421 ctctgagagc accagctcgc gggcgatgag ccggttggac agtcctcggc cgatcagccg
      481 caacacctca cgctctcggg tagtgagtgg gtcacggtcg gtgaccgaat gcatcaccgc
      541 cgccgcgacc cgcgggtgca gtgacgcgcc tccgtggtgc accgaacgca gcgcgcccgc
      601 aagctcggcc ggtccgacgt ccttgagcaa gtagccagcc accccagccc gcacggcggg
      661 tagcaccgag tcctgcccgg agaacgaggt gagcacgacg accttgacgt cgggatgggt
      721 gtcgcgcagc cgccgcgcgg tttccagccc gctcagtccg ggcagcacga gatccagcag
      781 cacgatgtcc gggcgcatgg cggccactaa tgccagcgcc tgttccccac tgctcgcctc
      841 gccgaccacc tccatgtcct gctgcagttc gaggaaggtg cgcagtccgg tgcgcaccat
      901 ctcgtggtca tcgacgagca gcagccggat cagatcagcc agtgggcacc tccagcgtca
      961 cggtggtgcc cgccccaggt gcggtgtcca cctgcagggt gccgccgagc gtttcggcac
     1021 gttcccgcat gctggccagt ccaaggcgcc gggaggtgcg ggctgtgacc gcgaggtcga
     1081 acccactccc gtcgtcggac accgtcagcc ggaccgccac ggacacgtcc agccgcaccc
     1141 ggatccggtg cggcgaagcg tgctgcaagg cgttgccgat cgcttcgttg gccaccttga
     1201 gcacctcgca cccgacctcg cccgtatggc gaccttcccg accgtgcact tccaggagga
     1261 tcggcacgtc atgcacagcg cggagcagat ccacccgctt gcgtagcgcc acgccgagcc
     1321 cctcgcggtc cacgtccggc ggacgcagcg tgtctaccag ggctctcagc tcatccagcg
     1381 cggtccgcga caggtcacgc acccggtcca gatgggcgta cgccggggat ccggcacctg
     1441 ccacggtagc cgcggactcc gcggcgaggg tgaggctgaa cagggtctgg gtcaccgagt
     1501 cgtgcaggtc ccgggcaatg cgggtgcgct cgtccacgat ggagagttcg cggagccttt
     1561 cctggcgttg ggcgttgacc acggcc
//
LOCUS       DATADN010000215         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1379567, whole genome shotgun sequence.
ACCESSION   DATADN010000215 DATADN010000000
VERSION     DATADN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379567"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VHH53_02865"
     CDS             complement(<1..926)
                     /locus_tag="VHH53_02865"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226813.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEX2289141.1"
                     /translation="MNPADRHPLSELEHGTPFADRHIGPRPGELARMLATIGVDSLEE
                     LAQLALPPTVIDPYAASNLPAPATEAQALTELRERAARCHPRIPMIGMGYHGTVTPPV
                     IRRSVLENPAWYTAYTPYQPEISQGRLEALLNFQTMVADLTGLPVANASLLDEATAAA
                     EAMTLLRRAGRASSSRFVVDADTLPQTLAVLATRAEPLGIELVVTDVAAALPDGEFFG
                     VLLSHPGASGAVRDHAMLIEQAHQRGAAVAVATDPLALTLLRPPGEIGADVAVGSTQR
                     FGVPIGFGGPHAAYLDVRHGLERQMPGRLVGMS"
     gene            complement(1168..1701)
                     /locus_tag="VHH53_02870"
     CDS             complement(1168..1701)
                     /locus_tag="VHH53_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2289142.1"
                     /translation="MFPDSVLADPHGVTDQLLGYRGSAACQIAGITYRQLDYWARTGL
                     VVPSIRGASGSGSQRLYSFKDVLVLKVVKRLLDTGVSLQNIRLAVEHLRRHGVHDLAR
                     ITLFSDGTTVYECTSPEEVVDLLQGGQGVFGIAVSGAMREIAGSISQFPPERADGGHV
                     ATGEDELTKRRRTRRIS"
     gene            complement(1988..2461)
                     /locus_tag="VHH53_02875"
     CDS             complement(1988..2461)
                     /locus_tag="VHH53_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX2289143.1"
                     /translation="MSEMRVVGVRVELPANQPILLLREAEGERYLPIWIGSGEATAIA
                     LEQHGVQPARPMTHDLLRDVITALVRELKQVRITELQEGTFFAELVFDGNVRVSARPS
                     DSVALALRAGVPIHAEESVLSEAGLIIPDEQENEVEKFREFLDSVSPEDFRGADT"
BASE COUNT          419 a          872 c          818 g          408 t
ORIGIN      
        1 gacatcccca ccagccgacc gggcatctga cgctccaggc catgacggac gtcaaggtag
       61 gcggcatgcg gtccgccaaa gccgatcggt accccaaaac gctgggtgga gccgaccgcg
      121 acgtcggcgc cgatctcgcc cggtggacgg agcagggtca gcgccagcgg gtccgtcgcg
      181 acggctaccg cggcgccgcg ttggtgcgcc tgctcgatga gcatcgcgtg gtcgcgcacc
      241 gcaccggagg cacccggatg ggacagcaac acgccgaaga actccccgtc cggcagcgct
      301 gccgccacat cggtgacaac cagttcgata cccagcggct cggcacgggt ggccagcacg
      361 gccaaagtct gtggcagcgt gtcagcatcg acgacgaatc gggacgagct ggcccggccg
      421 gcccggcgca gcagggtcat cgcctcggcc gccgcggtgg cctcatcgag caacgaagca
      481 ttggccaccg gcaagccggt gaggtcggcc accatcgtct ggaagttgag cagcgcttcg
      541 agtcgaccct gggagatctc cggctggtaa ggcgtgtagg cggtgtacca ggccgggttc
      601 tccagcaccg aacggcggat caccggtggg gtgaccgtgc cgtggtaccc catcccgatc
      661 atcggtatcc gtgggtgaca gcgggcagct cgctcacgta actcggtcag cgcctgtgct
      721 tcggtggccg gagccggcag gttcgaggcg gcgtacgggt cgatcacggt cggtggcagg
      781 gccagctgcg ccagttcctc caacgagtcc acgccgatcg tggccagcat ccgagccagc
      841 tcgcccggcc gcggcccgat gtgccggtca gcgaacggtg tgccgtgctc cagctcagac
      901 agtgggtgcc gatcagcagg attcatcgcg gtggcctccg ggcctcagct cgaaccgtcg
      961 ggcgcagtgt ccgatcggtc ctccccctct gtcactaccc agaccccgtc ctgccgagaa
     1021 ctgggcgcct gagagattca ccggcttgcg ccggctttcc ccgtcggcgg ccgcacgggg
     1081 tcggcgactt cccaaccagc ctacttgaag gaccgggacg tctcgttctg tcgacgctaa
     1141 acagtgaggc gctacacagt tgatgcgtca gctgattcga cgggtccgcc ggcgcttggt
     1201 cagctcgtct tcgccagtcg ccacatggcc gccatcggca cgctccggcg gaaactggct
     1261 gatcgacccg gcgatctccc gcatcgcgcc gctcacggcg atgccgaaca ctccctgccc
     1321 tccctgcagc agatccacca cctcctcggg agaggtgcac tcgtagacgg tggtgccgtc
     1381 gctgaacaag gtgatacgag ccagatcgtg gaccccatgg cggcgcaggt gctctaccgc
     1441 caagcggatg ttctgcagcg agacacccgt gtccagcagc ctcttgacca ctttgaggac
     1501 cagcacgtcc ttgaacgagt acagccgctg gctacccgat ccggacgcac cgcggatcga
     1561 tgggacgact aggccggtgc gagcccagta gtccagctgg cggtaggtga ttccggcgat
     1621 ctggcaggcg gccgaaccgc gataccccag cagctgatcg gtcacgccgt gcgggtcggc
     1681 caggacggag tccgggaaca gggtgccttg ctcggccggc tcgtcggtcc cggacggtgt
     1741 gacgccatat gccgggcttt ccgctcggcc gtgcggtccg tcgaccaccc attcctccca
     1801 tggctcaagc cggtggtccg tacgggccac tcgctgtgca gcccgacggt aggctgccgc
     1861 agcgtagccg gtcaacgcgg cacgcgggag agcctcaacc tctacttcag cttgaggggg
     1921 tgaccagcat cgatcaggtg accgctgcgc gacagcgtcg actaggtatc caccccgcga
     1981 ctgttgacta cgtgtccgct ccgcggaaat cctccggcga aaccgagtcg agaaactcgc
     2041 ggaacttctc gacctcgttc tcctgctcat ccggaatgat caggccagcc tccgagagta
     2101 ccgactcttc ggcgtggatg ggcacccctg cccgcagtgc cagggcgacg gaatcactcg
     2161 gccgggcaga gacccgcacg ttgccgtcga agacgagctc ggcgaagaaa gtcccttctt
     2221 gcaactcggt gatccggacc tgcttgagct cacgtaccag cgcggtgatc acgtctcgaa
     2281 gcaagtcgtg ggtcatcggc cgagccggtt gcacgccatg ctgttccagg gcgatggcgg
     2341 tcgcctcccc agaaccgatc cagatcggca ggtagcgctc gccttcggcc tcccgcagca
     2401 gcaggatcgg ctggttcgcc ggcaattcca cccggactcc gacgacacgc atctcgctca
     2461 tcgcgttgcg cctccctccc gccgcacctt gtgggccagg tgccttgagg atcgtcg
//
LOCUS       DATADN010000216         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1080202, whole genome shotgun sequence.
ACCESSION   DATADN010000216 DATADN010000000
VERSION     DATADN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080202"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VHH53_02880"
     CDS             <1..751
                     /locus_tag="VHH53_02880"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX2289144.1"
                     /translation="MRDGSVPAKVVLAHDLLAVADEPLDPRFLLHGLAARYPSCWTFA
                     VVQMVGATPELLLRRRGTEVLSRVLAGTTWPHDSTDPEALAAELLGSAKDRGEHCYAV
                     DSLAQELRPFCTELLVPTAPSVLQLRNVLHLATEVRGVLHPAGPPLLTLADALHPTAA
                     VGGSPTPAAVSAIGELEAMDRGRYAGPVGWVDAAGDGELGIALRCAQVAGRFARLFAG
                     CGIVAGSDPDSEVAEAAAKLVPIRDALEGVG"
     gene            complement(760..2238)
                     /locus_tag="VHH53_02885"
     CDS             complement(760..2238)
                     /locus_tag="VHH53_02885"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX2289145.1"
                     /translation="MRNRALLIGPGLAVLLVLIAIVVFPWRSSDSRPDSEAPPPPAVA
                     FQPVPGADGIGDPYFPQAGNGGYDVTGYDIQIRYDPPSDRLEGHTTVTARATQDLSQF
                     NLDLRLPATAVAVDGQPASFRQDGGELQVTPAAAVRAGQPMSVLVDYVGVPSSIPGGG
                     RFAEPWVRTADGAVAVGEPDSAAWWYPSNDHPSDKATVAVTVTVPAGVEAISNGALLG
                     GPEPAGPGWERWRWQAGAPMATYLAFVAIGQYDLVRRDTEFGPYLAAYAQGLDPQVVD
                     AARASVERTPEITRFLAGIFGPYPFSNLGGVVPNAPGLGFALETQTRPVYSPVFWGGG
                     EPDVRVVVHELAHQWFGDSVSVSRWSDIWLNEGFATYAEWLYTERTGGRSAQQMAELT
                     YQRRPAGTEYWRTTPGDPGAADVFSGPVYTRGAMALQALRVEVGDEDFFAALRTWTTE
                     RRGGNGTVADFLAVIERVSNEQVDDVAQTWLFTPSRPPSPPT"
     gene            complement(2294..3754)
                     /locus_tag="VHH53_02890"
     CDS             complement(2294..3754)
                     /locus_tag="VHH53_02890"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX2289146.1"
                     /translation="MRIRVLIGLVLALLVTVAVIVYPWRSSESPSGPTAPSADDERPV
                     AGADGIGDPYFPLAGNGGYDVTGYDIQIRYDPATGRLEGNTTITARATESLSRFNLDL
                     RLAASAVTVDDRPAAIRQDSGELQVTPAAPVRAGQPMVVQVDYAGVPSEIPYGADEPW
                     MRTADGAIALGEPDSAAWWYPSNDHPSDKATVAVTVTVPAGVEAISNGALLGGPEPAG
                     PGWEKWRWKAGTPMTTYLAFVAIGQYDLVRRDTEFGPYLAAYAVGLDPQVADAARASV
                     ERTPEITRFLAGIFGPYPFSNLGGVVPNAPTMGFALENQTRPVYSPVFWGDSEPDVRV
                     VVHELAHQWFGDSVSVSRWSDIWLNEGFATYAEWLYTERTGGRSAQQTAAQTYALFPA
                     DSEYWLTPPGNPGAANVLTGAVYSRGAMALQALRGTVGDEDFFTALRTWTTKRRGGNG
                     TVADFLAVVERVSGEDVDDVAEAWLFSPTRPPAPPV"
BASE COUNT          605 a         1386 c         1339 g          593 t
ORIGIN      
        1 gatgcgcgac gggtcggtac ccgcgaaggt ggtgctggcg cacgacctgc tcgcagtcgc
       61 cgatgagccg ctggatccac gattcctgct gcacgggttg gccgcgagat acccgtcctg
      121 ctggacgttc gcggttgtcc agatggtggg cgccaccccg gagttgctgc tgcgccggcg
      181 cggcaccgag gtgctctcgc gggtgctggc cgggacgacc tggccgcatg acagcaccga
      241 ccccgaggcg ctggccgctg agctactcgg ctcggcgaag gaccgcggcg agcactgcta
      301 tgcggtggac tcgctggccc aggaactgcg gccgttctgc acggaactgt tagtccccac
      361 tgcgcccagt gtgctgcagc tgcgcaacgt gctgcacctg gccactgagg tgcgcggggt
      421 gctgcatccc gcaggtccgc cgttgctcac cttggccgac gcgctgcacc ccaccgccgc
      481 cgtcggcggc agccctaccc ccgctgcggt cagcgcgatc ggcgagctgg aggcgatgga
      541 ccgcggacgc tacgccggcc cggtgggctg ggtcgacgcc gccggggacg gcgaactggg
      601 tatcgcactg cgttgcgctc aggtcgcagg ccggttcgcc cggttgttcg ccggctgcgg
      661 gatcgtggcc ggctcggacc ccgactccga ggttgccgag gccgccgcca agttggttcc
      721 catccgcgac gcccttgaag gagtgggctg acgccagcgt caggtgggtg gtgacggtgg
      781 acggctgggg gtgaacaacc aggtttgagc gacgtcgtcc acctgctcgt tggagactcg
      841 ctcgatgacg gccaggaagt cggccaccgt tccgttgcca ccacgacgct cggtagtcca
      901 ggtacgcagc gcagcgaaga agtcctcgtc cccgacctcc actcgcaacg cctgcagtgc
      961 catcgcgccg cgcgtgtaga caggtccgct gaacacgtca gccgcgccgg gatcccccgg
     1021 tgtggtccgc cagtactcgg taccagcagg cctgcgttgg taggtcagtt cggccatctg
     1081 ctgcgcgctg cggccgccgg tgcgttcggt gtagagccac tccgcgtagg tggcgaagcc
     1141 ctcgttgagc cagatgtcgc tccagcggct caccgacacg ctgtcgccga accactggtg
     1201 ggccagctcg tgcaccacca cccggacgtc cggttcaccg ccgccccaga acaccggtga
     1261 gtagaccgga cgggtctggg tctccaaagc gaagcccaag ccaggggcgt tgggcaccac
     1321 gccgccgagg ttgctgaacg ggtagggacc gaagatcccg gcgaggaatc tggtgatctc
     1381 gggcgtccgc tccaccgacg cccgcgcggc gtcgacgacc tgcggatcga ggccttgagc
     1441 gtaggccgcc aggtacggac caaactcggt gtcccgccgg acgaggtcgt actgcccgat
     1501 cgccacgaac gccaggtagg tggccatcgg cgcgcccgcc tgccaacgcc acctctccca
     1561 acccgggccg gccggttccg gaccaccgag cagggcaccg ttggagatcg cctccacccc
     1621 agcgggcacc gtcacggtca ccgcgacagt cgccttgtcc gacgggtgat cattggacgg
     1681 ataccaccac gcggcgctat cgggctctcc cactgccacc gcaccgtcgg cggtgcgcac
     1741 ccacggctcg gcaaaacggc caccaccggg gatgctcgag ggcactccga cgtagtccac
     1801 cagcaccgac atcggctggc cggcgcgtac cgccgccgcc ggggtcacct gcagctcgcc
     1861 accgtcctga cggaaactcg ccggctggcc gtccaccgcg accgcggttg ccggcagccg
     1921 caggtccaag ttgaactggg aaagatcctg ggtggcgcgg gcggtgaccg tggtgtgccc
     1981 ttcgagccgg tcgctgggag ggtcgtaccg gatctggatg tcgtagccgg tgacgtcgta
     2041 gccgccgttt cccgcctgtg ggaaataggg gtcgccgatg ccgtcggcgc cgggaaccgg
     2101 ctggaaagcg accgcaggag gcggtggcgc ctcggagtcg ggacgggaat cggagctacg
     2161 ccacggaaag accacgatcg cgatcagtac cagcagcacc gccaggcccg gcccgatcag
     2221 caatgcccga tttcgcatag tcacctccca gaactggatc ttagaccgtg cgggccgcct
     2281 gatcggatcg cgctcacaca ggtggtgcag gcgggcgggt cgggctgaac agccaggcct
     2341 cggcgacgtc atcgacgtcc tcgccggaga ctctctccac gacagccagg aagtcggcca
     2401 ccgtcccgtt gccccctcgc cgcttggtgg tccaggtgcg cagcgcggtg aagaagtcct
     2461 cgtcccccac tgtcccccgt agcgcctgca gcgccatcgc gccgcggctg tacaccgctc
     2521 cggtgagcac gttggcagcg ccgggattcc ccggaggggt cagccagtac tcggaatcgg
     2581 cgggaaacag cgcgtaggtc tgcgccgcgg tctgctgagc gctgcggccg ccggtgcgtt
     2641 cggtgtagag ccactcggcg taggtggcga agccctcgtt gagccagatg tcgctccagc
     2701 ggcttaccga gacgctgtcg ccgaaccatt ggtgggccag ctcgtgcacc accactcgca
     2761 cgtccggttc actgtcgccc cagaacaccg gtgagtagac cgggcgggtc tggttctcca
     2821 gcgcgaaacc catggtggga gcgttgggta ccacgccgcc gaggttgctg aacgggtaag
     2881 gaccgaagat cccggcgaga aacctggtga tctcaggcgt ccgctccacc gacgcccgcg
     2941 cagcgtcagc gacctgcgga tcgagcccca cagcgtaggc ggccaggtac ggaccgaact
     3001 cggtgtcccg gcggacgagg tcgtactgcc cgatcgccac gaacgccagg tacgtggtca
     3061 tcggcgtgcc cgccttccag cgccacttct cccaacccgg cccggccggt tccggaccac
     3121 ccagcagggc cccattggag atcgcctcca caccagcggg caccgtcacg gtcaccgcga
     3181 cagtcgcctt gtccgacggg tgatcgttgg acgggtacca ccacgcggcg ctatcgggct
     3241 ctcccagcgc gatagcgccg tcggcggtgc gcatccacgg ctcgtcggca ccgtacggga
     3301 tctccgaagg caccccggcg tagtccactt gcacgaccat cggctgacca gcgcgcaccg
     3361 gcgccgccgg agttacctgc agctcgccac tgtcctgacg gatcgcggcg ggccggtcgt
     3421 cgaccgtgac ggccgaggcc gccagtctca aatcgaggtt gaaccgggag aggctttcag
     3481 tggcccgggc agtgatcgta gtgttgccct ccagccggcc ggtggcaggg tcgtaacgga
     3541 tctggatgtc gtagccggtg acgtcgtagc cgccgtttcc cgccagcggg aaatagggat
     3601 ccccgatgcc gtcggcgccg gcgaccggcc gctcatcgtc cgcggatggc gcggtcggcc
     3661 cggaagggga ctccgagctg cgccacgggt acacgatgac cgcgaccgtg acgagtagcg
     3721 ccagcaccag tccgatcagc acgcggattc gcatgaacga cctcccggac tgcgacctta
     3781 ggccctgttt gcggacgtcc gaccgacggc catcgccacc gcggcccgga ttcgtccatg
     3841 taacgcccgc agcccggccc gctcagtgcg cacctccagc actcgcgtcc tcggggctgg
     3901 tgacagcgcc gcgggcagtt cgt
//
LOCUS       DATADN010000217         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_778562, whole genome shotgun sequence.
ACCESSION   DATADN010000217 DATADN010000000
VERSION     DATADN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778562"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VHH53_02895"
     CDS             <1..735
                     /locus_tag="VHH53_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289147.1"
                     /translation="HLLLAGLPLLAISAQVFGLVSMNTTAALVIIPLAATLAVLAVFA
                     PHPTDRIVGRGLVSGMVACVVYDIFRLDTVYLLGWWDDFIPTMGTWITDGAETRSGAV
                     VGYLWRYVGDGGGIGIAFFIVALAVGLHQRCRRDVVLAAVAFAVFPVWTGLIATVALA
                     PRGEQLMFPLTATTVSLSLIGHLIFGLVLGLGFWHSRSVARLWPWPPLLPTARGPAYH
                     GVSPATYEAWQRRLDSRPARRVRTPR"
     gene            complement(719..1507)
                     /locus_tag="VHH53_02900"
     CDS             complement(719..1507)
                     /locus_tag="VHH53_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2289148.1"
                     /translation="MLILAAVVLAVLALAWIFQRRLIYLPDSAAVPPAAEVVPGARDV
                     TVRTEDGLALGAYLVPPSGADRGMTVLVAPGNAGSRVLRVPLAQRLAARGFGVLLIDY
                     RGYAGNPGSPSEQGLVADGHAAYQYLVRQAGVPPQRLVYFGESLGAAVVTRLAVEHPP
                     AALVLRSPFTDLASVGAVHYPLLPVRQLLRDRFPVTEPIARVRVPTVVIYGAQDTIVP
                     PEQSRAVAAAAGGPIRTVEVAGADHNDPALLVGDELIDAITAVS"
     gene            complement(1534..2475)
                     /locus_tag="VHH53_02905"
     CDS             complement(1534..2475)
                     /locus_tag="VHH53_02905"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX2289149.1"
                     /translation="MSCTVQAPATPRTRHRGGVLRGYLALTKPRVIELLLVTTIPAML
                     LAARGVPSLWLIAATLAGGIMAAGSANALNCVADADIDAQMKRTRARPLVTHEMPIRH
                     ALIFGIALGVASFGWLWATTNLLSALLAVATILFYVFVYTLLLKRRTAQNIIWGGAAG
                     CMPVVIGWSAVTGTVGWPALVMFGVIYFWTPPHTWALAMKFREDYQRVGVPMLPAVAS
                     PVSVSRRIVVFSWLMVGWSLLLVPVTSWVYAVVAVLAGGWFVAMAHRLHAAVRRGAAV
                     NPMRLFHLSNTYLALVFVAIAVDSVLGWAQLTSAGLI"
     gene            2700..>3282
                     /locus_tag="VHH53_02910"
     CDS             2700..>3282
                     /locus_tag="VHH53_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2289150.1"
                     /translation="MSVIDDLTQLTTPRVPDDWTEVDTRAVDTIRVLAADAVQRVGSG
                     HPGTAMSLAPLAYTLFQRILRHDPTDPAWTGRDRFVLSAGHSSLTLYLQLFLCGYGLE
                     LEDIEQLRTWDSKTPGHPEFGHTVGVEITTGPLGQGLASAVGMAMANRRERGLFDPDA
                     APGESVFDHHIYVVASDGDIEEGVTSEASSLAGR"
BASE COUNT          543 a         1192 c         1080 g          467 t
ORIGIN      
        1 cacctcctgc tggcggggct accgttgctc gcgatctccg cgcaggtctt cggtctcgtc
       61 tcgatgaaca ccaccgcagc gctggtgata atcccgctgg cggccacgct cgcggtactg
      121 gcggtgttcg cgccgcaccc gaccgaccgg atcgtcgggc gcgggctcgt ctcgggaatg
      181 gtcgcctgcg tggtttacga catcttccga ctcgacacgg tctacctgct cggatggtgg
      241 gacgacttca tcccgacgat gggcacgtgg atcaccgacg gtgccgagac ccgaagcggc
      301 gccgtggtcg gctacctgtg gcgctacgtc ggcgacggcg gcgggatcgg catcgcgttc
      361 ttcatcgtcg cgctggctgt gggcctgcac caacggtgcc ggcgcgacgt cgtgctggcg
      421 gcggtggcct tcgcggtctt cccggtctgg accggactga tcgcgaccgt cgcgctggcc
      481 ccacgcggcg agcaattgat gttccccctt accgccacca cggtctcgct ctccctgatc
      541 gggcacctga tcttcggcct cgtcctcggc ctcggcttct ggcactcgcg cagcgtggca
      601 cggttgtggc cctggccgcc cctgctaccg accgctcgag ggcctgctta tcacggggtc
      661 agccccgcca cctacgaggc ctggcagcga cggctcgaca gtcggcccgc tcggcgcgtc
      721 aggacaccgc ggtgatcgcg tcgatgagct catcgccgac caacagcgcg gggtcattgt
      781 gatcggcacc ggcaacctcg accgtccgga tcggaccgcc cgccgccgcg gccacggctc
      841 ggctttgctc cggcggcacg atggtgtcct gtgcgccgta gatgacgacg gtgggcaccc
      901 gcacccgcgc gatcggctcc gtgaccggga accggtcccg cagcagctgc cggaccggca
      961 gcagcgggta gtgcaccgct cccacggagg cgagatcggt gaatggggaa cgcagcacca
     1021 gcgcagcggg cgggtgctcg acggccagcc gggtcaccac cgccgccccc aggctctccc
     1081 cgaagtacac cagccgctgc gggggcaccc cggcctgccg caccaggtac tggtacgccg
     1141 cgtgcccgtc agcgaccagc ccctgctccg acgggctacc cggattgccg gcgtacccgc
     1201 ggtagtcgat cagcagcacc ccgaaccccc gcgccgccaa ccgctgcgcg agtgggacgc
     1261 ggagcacccg agatccggcg ttgcccggcg ccacgagcac ggtcatgccc cgatcagcgc
     1321 cgctcggcgg cacgaggtag gcgccgagcg ccagcccgtc ctcggttcgc acggtgacgt
     1381 cgcgggcacc gggcactacc tcagcagccg gcggcacagc ggcactatcg ggcaggtaga
     1441 tcaggcgccg ctggaagatc cacgccaggg ccagtaccgc aagcaccacg gccgccagta
     1501 tcagcacgcc ccgcagcaca ggaccaatag gtatcagatc agcccagcag aagtcagctg
     1561 agcccaaccc agcaccgagt ccaccgcgat cgccacgaac accagcgcca ggtaggtatt
     1621 ggacaggtgg aacagccgca tggggttgac ggccgcacca cgacgcaccg cggcatgtaa
     1681 ccggtgtgcc atcgccacga accaaccacc ggccagcacc gccaccactg cgtagaccca
     1741 actcgtgacc ggcaccagca gcaggctcca gcccaccatc aaccagctga acacgacgat
     1801 gcgccgggat acggacactg gggaggccac cgcgggcaac atcggcacgc ccacccgctg
     1861 gtagtcctcg cggaacttca tcgccagcgc ccaggtgtgc ggcggggtcc agaagtagat
     1921 caccccgaac atcaccagcg ctggccaacc cacggtgccg gtgaccgccg accagccgat
     1981 caccaccggc atgcacccag cagcgccacc ccagatgatg ttctgtgcgg tgcggcgttt
     2041 gagcagcagc gtgtagacga agacgtagaa caggatcgtc gcgactgcga gtagcgccga
     2101 gagcaggttg gtagtggccc acaaccagcc gaaggaggcc acccccagag ctatcccgaa
     2161 gatcaacgca tgccggatcg gcatctcgtg ggtgaccagc ggccgggccc gggtccgttt
     2221 catctgggcg tcgatgtcgg catcggcgac acagttcagc gcgttcgcac tgcctgcggc
     2281 catgatgccg cccgccaggg tggccgcgat gagccacagg gaaggcaccc cacgagcagc
     2341 gagcaacatc gccgggatgg tggtgaccag gagcagctcg atcactcgcg gcttggtcag
     2401 cgcaaggtag ccgcgtagca cgccgccgcg gtgccgggtg cgcggtgtcg caggagcctg
     2461 gacggtacag ctcaccgcgg ctcctcgtct gcgttgatcg ggactgattg aggctggtgg
     2521 gggcgggtac tcgaatgccg atggtaatcg cgtggcgcgc gtcacctcct gacgggggcg
     2581 acgagagaag ttcagatcgc ggtctggctc cttctcgcta gggtgggaag tcacgggccg
     2641 gcctgcacag gccacccctc accacgaccg ccgcgccgag cacagtcagg agcacgagtg
     2701 tgtccgtcat cgacgatctg acccagctca ccaccccccg ggtgcccgac gactggaccg
     2761 aagtggacac ccgcgcggtg gacaccatcc gggttctcgc cgctgacgct gtgcaacgcg
     2821 tcggcagcgg tcacccgggc accgccatga gcctcgcccc attggcctac acgctgttcc
     2881 agcggatatt gcggcacgac ccgaccgacc ctgcctggac cggtcgggac cggttcgtgc
     2941 tgtccgccgg acactccagc ctcaccctgt acctgcagct gttcctgtgc ggctacgggc
     3001 tggagctgga ggacatcgag cagctacgga cctgggactc caagaccccg ggccaccccg
     3061 agttcgggca caccgtcggc gtggagatca ccaccgggcc gctcggccag ggcctggcct
     3121 ccgcggtcgg catggcgatg gccaaccgcc gcgagcgcgg tctgttcgac ccggacgccg
     3181 cacctggcga gagcgtgttc gaccaccaca tctacgtggt cgcctccgac ggtgacatcg
     3241 aggagggcgt cacctccgag gcctcgtcgc tcgccggacg cc
//
LOCUS       DATADN010000218         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_929776, whole genome shotgun sequence.
ACCESSION   DATADN010000218 DATADN010000000
VERSION     DATADN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929776"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VHH53_02915"
     CDS             complement(<1..1086)
                     /locus_tag="VHH53_02915"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2289151.1"
                     /translation="MAKHARPTRYLFVTGGVASSLGKGLTASSLGQLLTARGLRVTMQ
                     KLDPYLNVDPGTMNPFQHGEVFVTEDGAETDLDIGHYERFLDRDLGCNANVTTGQVYS
                     AVIAKERRGEYLGDTVQVIPHITDEIKSRIMAMSEPGDGGRPPDVVITEIGGTVGDIE
                     SLPFLEAARQVRHDVGRDNCFFLHVSLVPYLAPSGELKTKPTQHSVATLRSIGIQPDA
                     LVCRADREIPEGLKTKIAAMCDVDIDGVVAAPDAPSIYDIPKVLHSEGLDAYVVRRLG
                     LPFRDVDWTEWGDLLDRGHAPTELVLIALVGKYVDLPDAYLSVIEALRAGGFAHRAKV
                     EIHWVPSDTCETPAGAQAALHGMDGVLV"
     gene            complement(1251..>1867)
                     /locus_tag="VHH53_02920"
     CDS             complement(1251..>1867)
                     /locus_tag="VHH53_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HEX2289152.1"
                     /translation="AGATVTGELQLTEAFTDPSRSNQLSELTTRLLPAGLRLPTAPDA
                     GTLSGGLLGSLLLTNPQNGKAQATPAEATAVIAGLGDGGFVRVGPQPQPAQLAVVLTG
                     GASRGDGSGDRAGILARFATQLDRSGAGAVLAGRPGSGEGNGPIGVVRADTAATSVLS
                     TVDDVSTSAGRVVTVLALAEQAEGRAGRYGTGGNAQAVVPAIQG"
BASE COUNT          317 a          656 c          601 g          293 t
ORIGIN      
        1 caccaacacc ccgtccatac cgtgcagggc ggcctgcgcg ccggccggag tctcgcaggt
       61 gtccgaaggc acccagtgga tctcgacctt agcccggtgc gcaaagcccc cggcccgcag
      121 cgcctcgatc accgacaggt aggcgtccgg caggtcgacg tacttgccta ccagggcgat
      181 cagcaccagc tccgtcgggg catgcccccg gtccagcagg tcaccccatt ccgtccagtc
      241 cacgtcccgg aaaggcaacc cgagccggcg caccacatag gcatccaggc cctcggaatg
      301 cagcaccttg gggatgtcgt agatcgacgg tgcgtcgggg gctgcgacca ccccgtcgat
      361 gtcaacatca cacatcgctg cgatcttagt cttgagccct tccgggattt cccgatcggc
      421 acggcagacc agcgcatcag gctggatacc gatgcttcgc aaggtcgcca cggagtgctg
      481 ggtgggcttg gtcttcagct caccggaagg ggcaaggtag ggcaccagcg agacgtggag
      541 gaaaaaacag ttgtcccggc cgacgtcgtg acgcacctgg cgagcggctt ccaggaacgg
      601 tagcgactcg atgtcaccca ccgtcccacc gatctcggtg atcaccacat ccggtggccg
      661 gccaccgtct ccgggctcgg acatggccat gatccgggac ttgatctcgt cagtgatgtg
      721 cgggatcacc tgcacggtgt cgccgaggta ttccccccgg cgctccttgg cgatcaccgc
      781 cgagtacacc tgacccgtgg tgacgttggc attgcagccg aggtcgcggt ccaggaaccg
      841 ttcgtagtgc ccgatgtcca ggtcggtctc ggcaccgtcc tcggtgacga acacctcacc
      901 gtgctggaac gggttcatcg tcccgggatc gacgttgaga tagggatcga gtttctgcat
      961 cgtcacccgc aggccccgag cggtcagcag ctggccaagg ctggatgcgg tgagcccctt
     1021 gcccagcgag gaggcgacgc ctccggtgac gaaaaggtac ctggtggggc gagcgtgctt
     1081 cgccaagacc gtctcccgtg gtccggaccg gcccttccat cgatgaccgg ttatgcggta
     1141 gctggcggca cgccaccgac cacctggcgg cacgccaccg accacggagt ctcacggtaa
     1201 cactgtcact caaggtagtc gctgggccct catggtaacc ctcgcgccga tcaaccctgg
     1261 atggcgggaa ccacagcctg cgcgttgccg ccggtaccgt accgtccggc ccgaccctcc
     1321 gcctgctcgg ccagcgcgag aaccgtcact actcgcccag ccgacgtgct gacgtcgtcg
     1381 acggtggaca gcaccgaggt cgccgcggtg tcggctcgca ccacgccgat cggaccgttg
     1441 ccctcacctg acccaggcct accggcgagc accgcacccg cgccggagcg gtccagctgg
     1501 gtcgcgaagc gggccaggat gccggcccgg tcaccgctgc cgtcgccacg gctggcgcca
     1561 ccggtgagca ccaccgcaag ctgagctggc tgaggttgcg ggccgacccg gacgaaacct
     1621 ccgtcaccga gccctgcgat gactgcagtg gcctcagccg gcgtagcctg cgctttcccg
     1681 ttctgcgggt tggtcagcaa cagcgagccg agcagcccgc cggacagtgt cccggcgtcc
     1741 ggcgcggtgg gcagtcgcaa cccagccggc agcagccggg tggtgagctc gctgagttga
     1801 ttcgagcgag acgggtcggt gaacgcctca gtcaactgca gctcaccagt gacagtggca
     1861 ccggcgt
//
LOCUS       DATADN010000219         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1226507, whole genome shotgun sequence.
ACCESSION   DATADN010000219 DATADN010000000
VERSION     DATADN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226507"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VHH53_02925"
     CDS             complement(<1..1065)
                     /locus_tag="VHH53_02925"
                     /inference="COORDINATES: protein motif:HMM:NF036298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5336 domain-containing protein"
                     /protein_id="HEX2289153.1"
                     /translation="MTYSPGSPGYPPANQPTTQFSAPTQHFGKLPEPPAAGEGPNKLP
                     AYLLMVVAALGLLVYLCNFGPIFEVNASDFLGQGGTVSGSTLGIGLAVISALAAGLLA
                     GVTLLSKGRTYVAIAAVLSVLALLLVIAELINKPSEASIGWALYALIVLSLLQAGSAV
                     AALLFDTGVLTPPTPRPKYDQQQQYGQQQYGQQQYGQQPYGQPRQYGQQAPPGYGQGP
                     QYNPYAQQGQPQYGQPGQYGGQPGQYGQPQQYNPYGQPGQYAGTPFSGEAAGLGPDDG
                     GRVSRPGLMVLSLVLLILSALPFLAAGVLLLAAPLSADTIPPGYEQQLAQAGLTPEMA
                     VSALRGVAAVILVVALLYVLF"
     gene            complement(1209..2339)
                     /gene="sfnG"
                     /locus_tag="VHH53_02930"
     CDS             complement(1209..2339)
                     /gene="sfnG"
                     /locus_tag="VHH53_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfone monooxygenase SfnG"
                     /protein_id="HEX2289154.1"
                     /translation="MTTERIADHVKFAYWVPNVSGGLVTSDIEQRTDWNYAYNAKLAK
                     TAENNGFEYALSQVRYEASYGAEYQHESTSFSLALLLATERLKVIAAVHPGLWQPGVL
                     AKLGATADHLSNGRFAVNVVSGWFKDEFTHLGEPWLEHDERYRRSAEFLQVLRKIWTE
                     DDVDFRGDFYRIHDFTLKPKPANTPQRPNPELFQGGNSTAARRNGGRHADWYFSNGKD
                     FDGITERVAEVRDHARGAGREVKVGLNGFIIARDTEKEARDTLREIIEKANKPAVDGF
                     RNAVQQAGNSTADKKGMWADSGFEDLVQYNDGFRTQLIGTPEQIAERIAAYRKRGVDL
                     ILGGFLHFQEEIEYFGARVLPLVREIEAAEASSADSPVLATV"
BASE COUNT          347 a          777 c          851 g          404 t
ORIGIN      
        1 gaacaggacg tagagcaggg ccaccacgag gatgaccgcg gcgacccccc gcagggccga
       61 gacggccatc tccggggtga ggcccgcctg cgcgagctgc tgctcgtacc ccggcgggat
      121 cgtgtcggcg ctcagcggcg cggcgagcag cagcacgccc gccgcgagga acgggagtgc
      181 gctgaggatc agcaggacga gtgacaggac catgagcccg ggccgcgaga cccggccgcc
      241 gtcgtcgggt ccgaggccgg cggcctcacc gctgaacggg gtgcccgcgt actgaccggg
      301 ctggccgtac gggttgtact gctgcggctg gccgtactgg ccgggctgcc cgccgtactg
      361 gccgggctgc ccgtactgtg gctgcccctg ctgcgcgtac gggttgtact ggggaccctg
      421 gccgtacccg ggcggcgcct gctgcccgta ctggcggggc tgtccgtacg gttgctggcc
      481 gtactgctgc tgaccgtact gctgttggcc gtactgctgc tgctggtcgt acttgggccg
      541 cggcgtgggc ggggtgagga caccggtgtc gaagagcagc gcggccacgg ccgatccggc
      601 ctgcagcagc gacagcacga tcagggcgta gagcgcccag ccgatcgagg cctcggacgg
      661 cttgttgatc agttctgcga tcaccagcag caacgccagc accgacagca cggccgcgat
      721 ggcgacgtag gtgcggccct tggacagcag cgtcaccccg gccagcagac cggcggccag
      781 cgccgagatg accgcgagcc cgatgccgag cgtcgaaccg ctgacggtgc cgccctggcc
      841 gaggaagtcc gaggcgttga cctcgaagat cggaccgaag ttgcagaggt agacgagcag
      901 gccgagggct gcgaccacca tgagcaggta ggcgggcagc ttgttcggac cttcgccggc
      961 ggccggcggc tcgggcagct tgccgaagtg ctgcgtcggc gccgagaact gggtggtggg
     1021 ctgattggcc ggtggatatc cggggctacc gggtgagtag gtcatgacct ctcctgtgct
     1081 gatcgggcgt ctgacgtggg caacacgtgc gcatgaacac ggaccacctg agcaattcgc
     1141 tctcccacgc tagcgcaccc acgctcgtac ccggcgcacg acggtgtcta atccgccccg
     1201 ttgccgcctc acaccgtggc gagcaccggg ctgtccgcgg aggacgcttc ggccgcctcg
     1261 atctcgcgca ccagcgggag cacccgggca ccgaagtact cgatctcctc ctggaagtgc
     1321 aggaagccgc cgaggatcag gtcgacgccg cgtttgcggt atgccgcaat gcgttccgcg
     1381 atctgctcgg gggtgccgat gagctgtgtg cggaagccgt cgttgtactg gaccaggtcc
     1441 tcgaagcccg aatccgccca catccccttc ttgtcggccg tcgagttccc ggcctgctgg
     1501 accgcattgc ggaacccatc gaccgcaggc ttgttcgcct tctcaatgat ttcgcggagc
     1561 gtgtctctcg cctccttctc ggtgtcgcgg gcgatgatga aaccgttcag cccgaccttg
     1621 acctcccggc cggccccacg ggcgtggtcg cgcacctcgg cgacccgttc ggtgatgccg
     1681 tcgaagtcct ttccgttgga gaagtaccag tcggcgtggc ggccgccgtt gcgccgggcc
     1741 gccgtggagt tgccgccctg aaagagctcc gggttgggcc gctgcggcgt gttggccggt
     1801 ttcggtttga gcgtgaagtc gtggatgcgg tagaagtcgc cgcggaagtc cacgtcgtcc
     1861 tcggtccaga tcttgcgtag cacctgcagg aactcggcgc tgcggcggta gcgctcgtcg
     1921 tgttcgagcc acggctcgcc gaggtgggtg aactcgtcct tgaaccagcc cgacacgacg
     1981 ttgaccgcga agcggccgtt ggacaggtga tcggcggtgg cgcccagttt ggcgagcacg
     2041 ccgggctgcc acaggccggg atggacggcg gcgatgacct tgagtcgctc ggtggccagc
     2101 agcagcgcca acgagaagct cgtcgactcg tgctgatatt cggcgccgta gctggcctcg
     2161 tagcggacct ggctcagcgc gtattcgaag ccgttgttct ccgcggtctt ggccagcttg
     2221 gcgttgtacg cgtagttcca gtccgtgcgc tgctcgatgt cgctggtgac caggccgccg
     2281 ctgacattgg gcacccagta ggcgaacttg acgtggtcgg cgatgcgttc ggtcgtcatg
     2341 ccgacatggc atcgtgccgg ccggcgaccg tcaccggta
//
LOCUS       DATADN010000220         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1380264, whole genome shotgun sequence.
ACCESSION   DATADN010000220 DATADN010000000
VERSION     DATADN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380264"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VHH53_02935"
     CDS             complement(<1..751)
                     /locus_tag="VHH53_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289155.1"
                     /translation="MASKSLSEVSSRERALLDALVSGCVIECSELSVEQLAASENPRY
                     AIRGELIRELVLGRGGVRFDPLGVRIYGARITGTLDLTHVAATVGIELRGCYFDHAVF
                     LTGARLPWLTLSASHLPALDGDGLRVQGDVLLGEGFTATGHGEEGAVRLLGAHISGQL
                     NLNGARLANDAGPALIADGLHVYGDVFGGGFVATGHGELGAVRLPGAHVSGQVNLNGA
                     DLANQAGPALIGDGLRVDGGLVLAEGFTATGH"
     gene            842..1447
                     /gene="nadD"
                     /locus_tag="VHH53_02940"
     CDS             842..1447
                     /gene="nadD"
                     /locus_tag="VHH53_02940"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX2289156.1"
                     /translation="MFGRRLGVMGGTFDPIHHGHLVAASEVQARFGLDEVVFVPTGIP
                     WQKDERDVSPAEDRYLMTVIATASNPRFSTSRVDVDRRAPTVTVDTLTDLRTLFPDHE
                     MFFITGADALAQILSWKRVDELFELAHLVGVTRPGFQLDGKHLPDHAVSLVEVPAMAI
                     SSTDCRQRIACDQPIWYLVPDGVVQYISKRKLYRGAAIDQL"
     gene            complement(1849..2652)
                     /locus_tag="VHH53_02945"
     CDS             complement(1849..2652)
                     /locus_tag="VHH53_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX2289157.1"
                     /translation="MFLHNPKMMYTVRVDQPNPAFAKMLLEQFGGPNGELAAAMRYFT
                     QGWNEPNGPRRSMLLDIATEELSHLEMVAQTLSMLLKGSPSELVDQVEGNYLGELLDG
                     KMPDYVNLSLNSGPNVLGGGGPRLTDSMGAPWTAAYIDTIGQPVADLHSDIAAEARAK
                     IVYERLIKQCDDPGCKDTLTFLMTREVAHQKMFQAALDAIMDNFPPGNLPGDQALGHA
                     YVADSGDFSGDGKPDGFELSQANSDWGFELDTNPVGHAAQQEVGSTPAS"
BASE COUNT          492 a          900 c          874 g          500 t
ORIGIN      
        1 agtgtccggt tgcggtgaat ccctctgcga gaaccagccc accatcgact cgaaggccgt
       61 cgccgatgag cgccggaccg gcttggttgg ccaggtcggc gccgttgagg ttgacttggc
      121 cgctgacgtg cgcgccgggc agccgcaccg cgccgagttc gccgtggccg gtggcgacga
      181 aaccgccgcc gaacacgtcg ccatagacgt ggaggccgtc ggcgatgagg gcggggccgg
      241 cgtcgttggc cagccgcgcg ccgttgaggt tgagttggcc gctgatgtgg gcgcctagca
      301 accgtactgc gccctcctca ccgtgcccgg ttgcggtgaa accctcgccg agcagtacat
      361 cgccctggac ccggagtccg tcgccgtcca gagcggggag atgcgaagcg ctcaaggtca
      421 accacggtaa acgggcgccg gtgaggaaca ccgcgtgatc gaagtagcaa cctcgcagct
      481 caattcccac tgtggccgca acgtgggtga gatcgagtgt tccagtgatc cgcgcgccat
      541 agatgcgcac acctagcgga tcgaaacgga caccaccgcg ccccagcacc agttcccgga
      601 tgagttcgcc acggatggcg tatctggggt tctcactcgc agctagctgc tcaacgctga
      661 gttcgctgca ctcgatgacg cagccagaaa ccaaggcgtc gagtagcgcc cgttctctcg
      721 atgagacctc gctgaggctc ttgcttgcca ccactgccca acggtcggcc gcctagtttt
      781 cagctcattg ctgctagagc tggcccgaaa gagcctaccg gacgtcgcct aagctgcgcc
      841 catgtttggg cggcgactcg gcgtcatggg cggcaccttc gatcccatcc accatggcca
      901 cctcgtcgct gccagcgagg tgcaggcccg gttcggattg gacgaggtcg tcttcgtgcc
      961 caccgggatc ccgtggcaga aagacgagcg tgacgtcagt ccagccgaag accgctacct
     1021 catgacggtc atcgccaccg cgtccaaccc tcggttctcc accagccggg tggacgtcga
     1081 ccgccgggct cccaccgtca ccgtggacac tctcaccgac ttgcgcacac tgttccccga
     1141 ccatgagatg ttcttcatca ccggggctga cgctctggca caaatcttgt cttggaaacg
     1201 agtcgacgag ctgttcgagc tcgcccacct ggttggggtg acccgcccag ggttccagct
     1261 cgacggcaag cacttgccag accatgcggt cagcctggtc gaggtgccgg ccatggcgat
     1321 ctcgtcaacg gactgccggc agcgcatcgc ctgcgaccag ccgatctggt acctggtccc
     1381 cgacggggtc gtgcagtaca tcagcaagcg caagctgtac cgaggagcag ctattgacca
     1441 gttataacag cctcgttggt acccatgtac aacatcggta caactcgcca tcagccgctg
     1501 ccgaccggtc agcagcggct gatgggcgcg atccgcgcgc ggcgtcgatg agcgttcgcg
     1561 tccgatcgag agcatcctgc tgcgaaataa tggcgttgca aggacccaga aggcgaacta
     1621 cgccagccgg gcacgtccag ccgagacagc cccgcagatg ctcgccgcgc cgcaccccgg
     1681 caggatgcgg tcgtgggcag cggatggccg gccggcaacc acccgcccgg cgtgatagct
     1741 cactcgccaa gaacggcgca acagccaggc caatgcccct tcactgccgg agctgattcc
     1801 ctttcaattg ctccgccacg ggtattctca acttgttgcg ggacttggtc aggatgctgg
     1861 tgtggatccg acctcttgct gggcggcgtg gcccacgggg ttggtgtcga gttcgaaacc
     1921 ccagtcgctg ttggcttggg agagctcgaa gccatcgggc ttaccgtcgc cgctgaagtc
     1981 gccggagtct gcgacgtagg cgtgacctag cgcctggtcg cccggcaggt taccgggtgg
     2041 gaaattatcc atgatcgcgt cgagcgcggc ctggaacatc ttctggtggg caacctcgcg
     2101 ggtcatgagg aaggtcaatg tgtccttgca gccgggatcg tcgcactgct tgatcagccg
     2161 ttcgtaaacg atcttcgcgc gggcttctgc ggcgatgtcg gagtgcaggt cagccaccgg
     2221 ctggccgatc gtgtcgatgt aggcggcggt ccagggagca cccatcgagt cggtgagtcg
     2281 cggacccccg ccacctaaga cgttcggtcc gctgttgagt gacaggttga cgtaatcggg
     2341 catctttcca tccagcagct caccgaggta gttgccttct acttgatcga cgagctcgga
     2401 gggggatcct ttgagcagca tggaaagggt ctgggcgacc atttccaggt gtgagagctc
     2461 ctcggtcgcg atgtcgagca gcatgctccg gcgcggaccg ttgggctcgt tccagccctg
     2521 ggtgaagtag cgcatcgcgg cggcgagctc cccgttgggg ccgccgaact gctccagcag
     2581 catcttggcg aacgcgggat tcggctggtc aacccgcacc gtgtacatca ttttcgggtt
     2641 gtgcaaaaac atcagacagg tttccttccg gtcgtcgcac actgggacag ccacggcact
     2701 accgaaaggg ccctggcaca aactccggag tacccgccct gaccgccccc acaccacaat
     2761 gatcac
//
LOCUS       DATADN010000221         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_174871, whole genome shotgun sequence.
ACCESSION   DATADN010000221 DATADN010000000
VERSION     DATADN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174871"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VHH53_02950"
     CDS             complement(<1..484)
                     /locus_tag="VHH53_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289158.1"
                     /translation="MLGITGTPDTWWVDRELGREPGCGAQRAATRRLLLRQGLALQMV
                     CAYEPERFLQDGLDYLRRYYRQEWDSSWDEYWTRHRLKQHHRECLAEQELSTFGARMR
                     TEHRAPTLADLRGALDRGRLAWISVDNDWGQVDCHAVLVYGQRGDVFDVYSPELSRNC
                     L"
     gene            complement(612..695)
                     /locus_tag="VHH53_02955"
     tRNA            complement(612..695)
                     /locus_tag="VHH53_02955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Leu,seq:caa)
     gene            880..1503
                     /locus_tag="VHH53_02960"
     CDS             880..1503
                     /locus_tag="VHH53_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2289159.1"
                     /translation="MTQHADVGAVPTEPDRTATTRRVLVAEDEALIRLDLVEILSEEG
                     YAVVGEAADGLQAVEQAERLRPDLVIMDVKMPKMDGIDAASIIAERRIAPVVILTAFS
                     QRELVERARDAGAMAYLVKPFGRKDLVPAVELAVSRFRELIALESEVAGLTDRLETRK
                     VVERAKGLLMARHGLTEPEAFRWIQRNAMDRRTPMKAVAQAVIESLS"
     gene            1674..>2819
                     /locus_tag="VHH53_02965"
     CDS             1674..>2819
                     /locus_tag="VHH53_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2289160.1"
                     /translation="MAQRTALRVAALLGTLSVVMAGCGGGNTAAPGGQGSAAGGDPSA
                     TTCVNPPQAKPSGQPSITPLKIGTLLPETGSLAILGPAEFAAVDVAINEVNAAGGVLG
                     NQVTHIRGDSGDDRTDTANQTVDRQLGQGAQAIIGAAASGVTKLVIDKITGAGVVQFS
                     PANTSDEFTCWPDKGMYFRTAPPDSLQAQALAALMAADGAQRISILARNDPYGSGLAD
                     KTAQNLRDAGIPQNQLQKIIYDPNATSYNTEIDDVRQFNPDAIAVIGFDESKKIITRM
                     SEVQIGPGQKRVYGTDGNMDSALGNGLPLGLLKGMKGTSPRTRLSGYFQQRLEAEDPT
                     LENYPYAGESYDAVMLIALAAEQAKSTRGVDVASEINGITKDGEKCTT"
BASE COUNT          520 a          911 c          924 g          464 t
ORIGIN      
        1 gcaggcagtt ccgtgagagc tccggcgagt agacgtcgaa cacgtcgccc cgctggccgt
       61 agaccaggac cgcgtggcag tcgacctggc cccagtcgtt gtcgacagag atccacgcca
      121 gacgaccgcg gtccagagcg ccacggaggt cggcgagcgt gggcgcccgg tgctcggtcc
      181 gcatcctggc gccgaaggtg ctcaactctt gctccgccag gcactcgcgg tggtgctgct
      241 tcagccgatg ccgtgtccaa tactcgtccc acgatgagtc ccactcctgg cggtagtagc
      301 gcctcaggta atcgaggccg tcctggagga accgctcagg ctcgtaggcg cacaccatct
      361 gcagggccag gccttgccgg agcagcaagc gcctggtggc ggcccgctgc gctccgcagc
      421 cgggctcccg accgagctcg cgatcaaccc accaggtgtc gggtgtgccg gtgatcccta
      481 acacgtatag aacgtttgca gcgacacagg tcaaacacga attctcatcc acctgacgaa
      541 ggtccagcgg ttctcgctgg cgcacgatcc ccgcctgctc ggtcatgttc cccgctcggt
      601 catgctggtt gtgccgggag cgggactcga acccgcatgt ccttacggac agacgctttt
      661 gaggcgtccg cgtatgccaa ttccgccatc ccggccagca catcccccaa cgtcagacca
      721 cccggatgct agcagcgcgc gggtgcccga gtgttgcagt gtcacggccg gggtgttggc
      781 gaaatcactg tacagaagat tcgtttagcc ccgactccga tcctggcacc gctatcgtgg
      841 caggtcacgg tgttggccga acggacggag gtctctccgg tgactcagca cgctgatgta
      901 ggtgcagtac ccaccgagcc ggaccgcact gccaccacac gtcgggtgct cgtcgccgag
      961 gacgaggcac tgatccggct ggacctcgtc gagatcctct ccgaggaagg ctacgccgtg
     1021 gtcggcgagg cagctgacgg cctgcaggcc gtcgagcagg ccgagcggct gcgccccgat
     1081 ctggtgatca tggacgtgaa aatgccgaag atggacggta tcgacgccgc gtcgatcatc
     1141 gccgagcggc gtatcgcgcc ggtggtgatc ctcaccgcat tcagccaacg tgagttggtg
     1201 gaacgggccc gtgatgccgg tgcgatggcc tacctggtca agccgttcgg ccgcaaggac
     1261 ctggtgcctg cagtggagct ggcggtgtcg cgcttccgcg aattgatcgc cctggagtct
     1321 gaagtggcgg gcctgaccga ccggctggag acccgcaagg tggtggagcg tgccaaaggg
     1381 ctgttgatgg cccggcacgg tctcaccgaa cccgaggcgt tccgctggat ccagcgcaat
     1441 gcgatggacc gccgcacccc catgaaagcg gtggctcagg cagtcatcga gagtctgtcc
     1501 tgatccggcg cgggacagcc cgctagggca gcagcaagtc tggccggatt acatcttcgt
     1561 cacgacaggg cggccgatcg tgccgtcgcg ccactggcag gattacggtc tgacgatcgc
     1621 atggccggac ggcgatgccg gcagcccagc cgttttgttc tgaggagaga tgcatggcgc
     1681 agcgcaccgc gctccgcgtg gcagccctgc taggcacctt gtcggtcgtg atggccggct
     1741 gtggtggtgg caacaccgct gcacctggtg gccagggatc ggccgcgggt ggcgacccat
     1801 cggcgactac gtgtgtgaac cccccgcagg ccaagccgag cgggcagccg agcatcacac
     1861 cgctgaaaat cggcacgttg ctgccggaga ccggcagcct ggccatcctg gggccggctg
     1921 agttcgccgc ggttgacgtc gcgatcaatg aggtcaacgc ggctggaggg gttctgggca
     1981 accaggtcac gcacatccgg ggcgactcgg gcgacgatag gaccgacacg gcgaatcaga
     2041 ctgtggaccg ccagctcggc cagggagccc aagcgatcat cggtgccgcc gcctctggag
     2101 tcacgaagct ggtcatcgac aagatcaccg gtgcgggcgt ggtccaattc tcacccgcca
     2161 acacctctga cgagttcact tgctggcccg acaagggcat gtacttccgg accgctccac
     2221 cggattcgct gcaggctcag gccctggcgg cgttgatggc cgccgacggt gcgcagcgga
     2281 tctccatcct ggcccgcaac gatccctatg gaagcggcct ggccgacaag accgcgcaaa
     2341 acctgcgtga cgccggtatc ccgcagaacc agcttcagaa gatcatttat gatccgaacg
     2401 cgacgtccta caacaccgag atcgacgacg tccggcagtt caacccggac gcgatcgcgg
     2461 tgatcggctt cgacgagtcc aagaagatca tcacgcggat gagcgaggta cagataggac
     2521 ccggtcagaa gcgggtctac ggcaccgatg gcaacatgga tagcgctctg ggcaatggcc
     2581 tgccgctggg cctgctcaag ggcatgaagg gtacgtcccc gcggaccaga ctgtcgggtt
     2641 atttccagca gcgcctggag gctgaagatc ccacgctgga aaattacccc tacgccggtg
     2701 aatcttacga tgctgtcatg ctcatcgcac tggccgccga gcaggcgaag tcgaccaggg
     2761 gagtcgacgt cgcctcagag atcaacggca tcaccaagga cggtgagaag tgcaccacc
//
LOCUS       DATADN010000222         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_779255, whole genome shotgun sequence.
ACCESSION   DATADN010000222 DATADN010000000
VERSION     DATADN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779255"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..700
                     /locus_tag="VHH53_02970"
     CDS             167..700
                     /locus_tag="VHH53_02970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2289161.1"
                     /translation="MADVQIAHTAWLIRGELQAIRILLDEAFNGDVTDDDYEHALGGM
                     HALVWEGSELIGHGSVIMRRLLHGGRALRTGYVEGVAVRADRRRRGHGAALMTALERV
                     IRGGYEIGALGSSDEAADFYAAREWQQWMGTASVIAPGGIERTAQEEGCIYVLPVSAE
                     LTPDGDLACDWRGGDVW"
     gene            856..1305
                     /locus_tag="VHH53_02975"
     CDS             856..1305
                     /locus_tag="VHH53_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2289162.1"
                     /translation="MPTSAVNHPVFAQIYVWLSHRMEPELAAHRRNLLAGLSGQVLEL
                     GAGNGLNFAHYPVGVTRVLAVEPEPHLRALAIDSAAKAAVPVEVVDGVADRLPAGESS
                     VDAAVASLVLCSVPDQASALHEIRRVLRPGGQLRFLEHVRAPSAAWG"
     gene            complement(1516..2406)
                     /locus_tag="VHH53_02980"
                     /pseudo
     CDS             complement(1516..2406)
                     /locus_tag="VHH53_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(2437..>2870)
                     /locus_tag="VHH53_02985"
     CDS             complement(2437..>2870)
                     /locus_tag="VHH53_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2289163.1"
                     /translation="RARVDAALAAVGGTAYADAPVGRLSGGEQQRLRVAQALVGDPAV
                     MLCDEPLLSLDLAHQQSVTRLIDRRRRKAATAVVFVTHEINPVLPLVDRVLYLVDGRF
                     RIGTPDEVMNSATLSQLYGADVEVIKVRGRLLVIGTHEVHP"
BASE COUNT          465 a          996 c          994 g          415 t
ORIGIN      
        1 cctctacttc agcgctgcct tccgccaggg tgcgctccac gcccgtctcg atgcgcgaac
       61 accccgggct cacccgtttc ccgtctgatt ccatatgtcg agcgtaggcc gctcaggtgg
      121 gagcgccctc gagaggtggg gccgggtcaa gcgccaggat ggcgatatgg ctgacgtaca
      181 gatcgcccat accgcctggt tgatccgggg ggaactgcag gctatccgga tactgctcga
      241 cgaggccttc aacggcgatg tcaccgacga cgactacgaa catgctcttg gcggcatgca
      301 tgcccttgtt tgggaaggca gcgagctgat cgggcacggc tcggtgatca tgcggcggct
      361 gttgcacggc gggcgcgcgc tgcgcaccgg gtatgtcgag ggcgttgcgg tccgtgcgga
      421 tcgccgccga cgtggccacg gcgcagccct gatgacagcg ctggagcggg tgatccgcgg
      481 gggatacgag atcggtgcgc tgggctcctc cgacgaggcc gcggactttt atgccgcccg
      541 cgaatggcag cagtggatgg gcacggcctc ggtgatcgca cccggtggga tcgagcgcac
      601 ggcccaggaa gaggggtgca tttacgtgtt gcccgtcagc gcggagttga cgccggatgg
      661 ggacctggca tgtgactggc ggggtggcga cgtgtggtag tggcacaccg cagttactca
      721 cgctggaggc tgatcagcca ggctgggacc tcgccggtgg tgaccgccta ggtggcgtag
      781 ggctcgtcac tccagcctgc cggcgcgggt gtgatagcgg tcgcggtcgc ggtcgcggtc
      841 gcgagagaat tcgatgtgcc gacctcagca gtcaaccatc cggtcttcgc ccagatctat
      901 gtctggttga gccaccggat ggagccagaa ctggctgcgc accggcgcaa cctgctggcc
      961 gggctcagcg ggcaggtgct cgaactcggt gcgggcaacg ggttgaactt cgcgcactac
     1021 ccggtggggg tcactcgcgt cctcgccgtc gaaccagagc cgcatctgcg cgcgctcgcc
     1081 atcgactcgg cagcgaaggc ggcggtgccg gttgaggtcg tggacggggt cgctgaccgg
     1141 ctgcccgccg gcgaatccag cgtcgacgct gccgtggcgt cactggtgtt gtgctcggtg
     1201 cccgaccagg catcggcgct gcacgaaatc cgccgggtgc tgcggccggg tggtcagctg
     1261 cggttcctcg aacacgtgcg ggccccctcg gcagcgtggg gctgagccca gcggctgctg
     1321 gacgcgacga tctggccggc gatcgctggt ggctgccaca cctatcgcga taccgcggcc
     1381 gcgatccgcc aggccagctt cacgatcgag caactgcagc cgctacgctt ccccaacctc
     1441 cgtctctcca caccaacctc cccccacatc ctcggtatcg cgtccctccc ggcgcagtcc
     1501 ggatgcggaa cgcggttatt gagtgtgagc gggtacgggg tgagtacggc cgatccggtg
     1561 cagacgcatg gcccctatag cccggcaggt taggtagatt acgaaggcga tcgcggtgac
     1621 gtagccgctg atcggggcgc cgggcgccag ctatgccacg atcccgccga gcacggcgac
     1681 ctccgcgaac agcgtggcca gcagtagtgc ggtaacgggg ttggcggtca cccgggcggc
     1741 tgcggcggct ggggtgatga gcagcgagag caccagcagg gcgccaaccg tgggcaccgc
     1801 gagcccggtc gccaccccga ccaacacggc gaacagcacc gacagcccgc gcaccgggac
     1861 gccgcgggcg cgagccacat cggcgtccac gctggtgaac agcagcggac gccacaccaa
     1921 cgccaggacc agcagcaccc ccgccgtgca gccggccagc agccatgcgt cgtttcggga
     1981 aactcccacg atctggccga acagcagcga cagctgattc gacgcccgcc cggggtacag
     2041 ccacaggaac agcccgccca ggcccagccc gaacgcgagc accgcgccga tcaccgagtc
     2101 gcgctcgcgt tcacgcaacc cgagcaggcc gaagaccagc gcagccacca ccgcgcccac
     2161 cagcgcacct acgctgacca gtgagctgct caccagcagg gccgctgcag cgccggtgaa
     2221 ggtcaactcg ctggtgccgt gcaccgcgaa ggacacctgc cgggccacca ccagcggtcc
     2281 cagcgcgccg gcgatcagtg ccagcagcgc gccagcgatc agggactgct ggacgaagtc
     2341 cagtctcaac agctccccgg tgccctgaaa ggtgaacagc cggcgcagga tctcgtcaat
     2401 gctcatggct cgaccctgac gagcaggtct tgcggctcac gggtgcacct cgtgtgtgcc
     2461 aatcaccagc agccgtcccc gcaccttgat cacctctacg tcggcgccgt agagctgcga
     2521 caatgtcgcg gagttcatca cctcgtccgg ggtgccgatc cggaaccggc cgtccaccag
     2581 gtacagcacc cggtccacca gcggcagcac cgggttgatc tcgtgcgtga cgaacacgac
     2641 cgccgtcgcg gccttccgcc gccgccggtc gatcagccgg gtgacggact gctggtgcgc
     2701 caggtccagc gacagcaggg gctcgtcgca cagcatcacc gcggggtcgc cgacgagggc
     2761 ctgcgccacc cgcagccgct gctgctcgcc gccggacagg cgcccgacgg gggcgtccgc
     2821 gtaggcggtg ccgccgacgg cggccagcgc ggcgtcgacc cgggcgcggc
//
LOCUS       DATADN010000223         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_327295, whole genome shotgun sequence.
ACCESSION   DATADN010000223 DATADN010000000
VERSION     DATADN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327295"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2758)
                     /locus_tag="VHH53_02990"
     CDS             complement(<1..>2758)
                     /locus_tag="VHH53_02990"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEX2289164.1"
                     /translation="IDELMAAADGIEFPIFVKAIAGGGGRGMRRVTSLRGLRDALEAA
                     SREAESTFGDPTVFLERAVIEPRHIEVQVLADSTGEVIHLYERDCSVQRRHQKVVEIA
                     PAPNLDPDLRARICADAVAFARQIDYVNAGTVEFLVDRAGNHVFIEMNPRIQVEHTVT
                     EEITDIDLVASQLRIAAGETLADLGLSQDAIVPHGAALQCRITTEDPANDFRPDIGTV
                     TAYRSASGAGVRLDGGTVYPGAQIGPHFDSLLVKVTCRGRDLGSAVLRARRAIAEFRI
                     RGVHTNIPFLLALLDEPDLQAGKVTTSFIEQRPYLLTTRQSADRGTRLLTYLGHMTVN
                     RPHGEPPELVDPMLKLPPIDLDAPPPTGSRHQLLALGPEGFARWLRTRDSVGVTDTTF
                     RDAHQSLLATRVRSKDLVAAAPYVARVTSQLLSLECWGGATYDVALRFLAEDPWERLA
                     ALREAVPNLCLQMLLRGRNTVGYTPYPTEVTAAFVEQAVETGLDIFRIFDALNDVSQM
                     RPAIDTVRETGRAVAEVALCYTADLSDPAEKLYTLDYYLRLAEEIVAAGAHVLAIKDM
                     AGLLRPPAARTLVTALRSRFDLPVHLHTHDTPGGQLATLLAAIDAGVDAVDAATASMA
                     GTTSQPSLSALVAATDHTERSTGLDLQAVCDLEPYWELVRKVYAPFESGLASPTGRVY
                     HHEIPGGQLSNLRQQAVALGLADKFEQIEEAYAAADRMLGRLIKVTPSSKVVGDLALH
                     LVGAGVEPKDFEADPARFDIPDSVIGFLRGELGDPPGGWPEPLRSKALKGRKDPKGIA
                     ELSAEDRKGLREDRARTLNRLLFAGPAADFEEHRESYGDTSVLPSKEFFYGLRPGQEH
                     AVDLEPGVRLLIQLHAIGDADERGMRTVMCTLNGQLRPLQTRDRSIDSEIPVAERANK
                     SDGN"
BASE COUNT          436 a          894 c          973 g          455 t
ORIGIN      
        1 tggttgccgt cggatttgtt ggctcgctcg gcaaccggaa tttccgagtc gatggagcga
       61 tcccgggtct gcaggggccg cagctgcccg ttgagcgtgc acatcacggt gcgcatgccg
      121 cgttcgtcgg catcgccgat cgcgtgaagc tggatcagca gtcgcactcc cggttcgaga
      181 tctactgcgt gctcctgacc gggacgcagg ccgtagaaga attccttgct gggcagtacc
      241 gaggtatcgc cgtaggactc gcgatgctcc tcgaaatctg cggcgggacc agcgaacagc
      301 agccggttga gggtgcgcgc ccggtcctca cgaaggccct tgcggtcctc agcggacagc
      361 tccgcaatcc ccttgggatc tttacgaccc ttgagcgcct tgctgcgcag gggttcgggc
      421 cagccaccgg ggggatcacc gagttcgccg cgcaggaatc cgatcaccga gtcggggatg
      481 tcgaaacgag cggggtcggc ctcgaagtct ttcggctcga caccggcacc gaccaggtgc
      541 agcgctaggt ccccgacgac cttggaggat ggagtcacct tgatcaacct gccgagcatg
      601 cggtccgccg ctgcgtaggc ttcttcgatc tgctcgaact tgtccgccag gcccaatgcc
      661 acggcttgct ggcgcaggtt ggacaactgg ccaccgggga tctcgtggtg atagacccgc
      721 ccggtgggcg aggccagacc ggactcgaag ggcgcgtaga ccttgcgcac cagttcccag
      781 tagggctcca ggtcgcagac cgcctgcagg tccagcccgg tgctgcgctc ggtgtggtcg
      841 gtggccgcga ccaacgcgga cagcgatggc tgcgacgtgg taccggccat cgaggcggtg
      901 gcggcatcga cggcgtccac cccggcgtcg atcgcggcga gcagggtggc cagctgccca
      961 ccgggtgtgt catgggtatg caggtgcacg gggagatcga accggctgcg cagcgctgtc
     1021 accagcgtgc gggccgcggg gggtcgtagc aggcccgcca tgtccttgat ggccagcacg
     1081 tgcgcgccgg ctgccacgat ctcctcggcc agccgcaggt aatagtccag cgtgtacagc
     1141 ttctccgccg gatcggacag atcggcggtg tagcacagcg ccacctcggc gacggcccgg
     1201 ccggtctcgc gcaccgtgtc gatggccggg cgcatctggg agacgtcgtt gagcgcgtcg
     1261 aagatccgga aaatgtccag gccggtctcg acggcctgct ccacgaaggc cgcagtcacc
     1321 tcggtcgggt acggcgtgta gccgacggtg ttgcgcccac gcagcagcat ctgcaggcac
     1381 aggttgggca ccgcctcgcg caatgcggcc agtcgctccc aggggtcctc ggcgaggaat
     1441 cgcagcgcca cgtcgtaggt cgcgccaccc cagcactcca acgacagcag ttgcgaggtc
     1501 acccgggcca cgtagggcgc cgctgcaacg aggtccttgc tgcgcactcg ggtagcgagc
     1561 agtgactggt gggcgtcgcg gaaggtggtg tcggtgacgc cgacactgtc gcgggtccgc
     1621 aaccagcgcg cgaagccttc cggtcccagc gcgaggagct ggtgccggga tccggtaggc
     1681 ggtggcgcgt ccaggtcgat cggcgggagc ttgagcatcg ggtcgaccag ctcggggggc
     1741 tcaccgtgcg gccgattcac cgtcatgtgg ccgaggtagg tcagcaaccg ggtgccgcgg
     1801 tcggcggact ggcgcgtggt gagcaagtac ggccgttgct cgatgaacga ggtggtgacc
     1861 ttgcccgcct ggagatccgg ttcgtcgagc agggcgagca ggaacgggat gttcgtgtgt
     1921 accccgcgga tccggaactc ggcgatcgcg cgccgcgccc gcagcacggc agaccccagg
     1981 tcccgtcccc ggcacgtcac cttgaccagc aaggagtcga aatgcggacc tatctgcgca
     2041 cccgggtata cggtgccgcc gtccagccga actccggcgc cgctggcgga gcggtacgcg
     2101 gtgaccgttc cgatgtccgg acggaagtcg ttggccgggt cctcggtggt gatccggcat
     2161 tgcagcgcgg cgccatgcgg cacgatcgcg tcttgggaca acccgaggtc ggccagcgtc
     2221 tcccccgccg cgatgcgcag ttgggaggcg accaggtcga tatcggtgat ttcctcggtg
     2281 acggtgtgct cgacttggat gcgcgggttc atctcgatga acacgtggtt gcccgcgcgg
     2341 tcgacaagga actccacggt gcccgcgttg acgtagtcga tctgccgggc gaaggccacc
     2401 gcatcggcac agattcgcgc ccgcagatca gggtccaggt tgggcgcggg ggcgatctct
     2461 acgactttct ggtggcgccg ctgtaccgag cagtctcgct catagaggtg gatcacctcg
     2521 ccggtcgagt cggcaaggac ctgcacctcg atatgccgag gttcgatgac ggcccgctcc
     2581 aggaacacgg tcgggtcacc gaacgtcgat tcagcctcgc gggaggccgc ctccagtgca
     2641 tcgcgcaggc cgcgcagcga ggtgaccctg cgcatgccac gcccgccgcc gccggcaata
     2701 gccttgacga agatcggaaa ctcgatcccg tccgcagcgg ccatcagttc gtcgatgt
//
LOCUS       DATADN010000224         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_20753, whole genome shotgun sequence.
ACCESSION   DATADN010000224 DATADN010000000
VERSION     DATADN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20753"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VHH53_02995"
     CDS             <1..308
                     /locus_tag="VHH53_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2289165.1"
                     /translation="GEVHLDPVVAQQLTRSLRTPRPQGLTNLTEREREVLGLLAQGLS
                     NKAIAKQLFISERTARTHVSSILTKLELASRTQAALWAIREGLSPTPSRFAGPEHPR"
     gene            405..824
                     /locus_tag="VHH53_03000"
     CDS             405..824
                     /locus_tag="VHH53_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2289166.1"
                     /translation="MSASEPGRAATGKLTVLVVDDDARVRRALRTLIESSPDLTVAAQ
                     AATETQTLQLDLQLEPDIVVLDLLLPAASNGLNALRELIRRGRPVVAISISGGLRAPA
                     LGAGADAFLEKGGREMDRLLDTIRAASPRPPPSSARA"
     gene            1360..>1921
                     /locus_tag="VHH53_03005"
     CDS             1360..>1921
                     /locus_tag="VHH53_03005"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX2289167.1"
                     /translation="MRTEVPTAVVAREQLLDHDAVRLFVRHADAGSKQEWIGPDADRP
                     LSAVGWRQANGLVGLLGTFPAARLLTSPALRCRQTVAPLVAAYGLDAEGVVELGTDSN
                     IDRLLGLLEAPETREAVLCTHGEVFRSLMDRLGGRGTVDPLDHRQWDKGATCILRGDP
                     RSRLSVSFLPPAVPLADTRQLAAQVGQ"
BASE COUNT          319 a          672 c          620 g          310 t
ORIGIN      
        1 gcggcgaggt ccatctggat ccggtcgtcg cccagcagct cacccgctcg ctgcggaccc
       61 ccaggccgca ggggctgacg aacctgaccg agcgcgagcg tgaggtgctg ggcctgctgg
      121 cccagggcct gtcgaacaag gccatcgcca agcaactgtt catcagcgaa cgtaccgccc
      181 ggacccacgt cagcagcatc ctcaccaagc tcgagctggc ctccaggacg caggctgccc
      241 tgtgggccat cagagagggg ctcagcccga cgccctcgcg gttcgctggg ccagagcacc
      301 ctcggtaggt cattcgtccc ctcacgggtc ggtccattga ccaatgccac ctcacgccgc
      361 ggcctggttg aatcgaccca aacccagccc cagaagggag cgcggtgtcg gccagcgaac
      421 ctgggagggc agcgaccggg aaactgacgg tcctcgtcgt tgacgacgac gctcgcgttc
      481 gccgggcgct gcggacgctg atcgaatcct ccccggatct caccgtggca gctcaggccg
      541 ccaccgaaac ccagaccctt cagctcgacc tgcagctgga gcccgacatc gtagtcctgg
      601 acctgttgct tcccgccgcc tccaacggcc tgaacgcact acgggaactg atccgtcgag
      661 gccgcccagt ggtcgccatc agcatctcgg gggggttgcg cgcccccgcc ctcggcgcag
      721 gcgccgacgc cttcctcgag aagggcggac gggagatgga ccggctcctt gacaccatcc
      781 gtgccgcatc accccgccca ccgcccagct cagcccgcgc ctgaacggtc gatgcatcgc
      841 accgatcgcc gatgcccgga aggggcattg ggctggttga gggaaccaga cgaagcctcc
      901 tataggcact agaccggtcg cgaggacgtg gccgaggtgg cggtcgaccg gcgagccgaa
      961 ccagccctgg agtccttgct cctactggac cctccaagaa ccatagccgg cgaggctgtg
     1021 gtgctctcgg agccgaccgg catgcctgga tggaacagtc ttccgataag ggcaggcggc
     1081 tacacggtca gccccttcgc tcggcagctg cttccacggt cgtcagcccg ctgcgtcggg
     1141 cctgaacttc ttcttggccc accaggccgg gcacgacgcc aagagcttgg cggctacgtc
     1201 aattgtcgcc ctggatcctg gtcagttcgc cgatgaccac tcgagcgacc atcgggagac
     1261 ttcactagcc cggctgctgc aataggagga ccagccgttc ccaagggttg acaggatcgt
     1321 acccgtctct ggacggcaga atccgcagag gcggccacga tgagaactga ggtgccgacg
     1381 gcagtggtcg ctcgtgagca gctgctggat cacgacgccg tgcgcctgtt cgtccgtcac
     1441 gccgacgccg gcagcaagca ggaatggatc gggcccgacg cggatcggcc gctgagtgct
     1501 gttggctggc ggcaggccaa cgggctcgtc ggtctcctgg gcacctttcc tgccgctcgt
     1561 ctcttgacga gccccgcact tcgctgtcgt cagacggtcg ccccactggt cgccgcgtac
     1621 gggcttgacg ctgagggcgt cgtggaactc gggaccgaca gcaatattga tcggctcctc
     1681 gggctgctgg aagcacccga gaccagagag gcggtgctct gcacccacgg tgaggtgttt
     1741 cggtctctca tggatcggct tggcggcagg ggtacggtcg accctctcga ccaccggcag
     1801 tgggacaagg gcgcgacctg catcctcaga ggcgacccac gttcgcggct gtcggtctcg
     1861 ttcctgccgc ccgcagttcc cctggccgat actcgacagc tggcggctca ggttggccag
     1921 c
//
LOCUS       DATADN010000225         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_930829, whole genome shotgun sequence.
ACCESSION   DATADN010000225 DATADN010000000
VERSION     DATADN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930829"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..591
                     /gene="rplJ"
                     /locus_tag="VHH53_03010"
     CDS             46..591
                     /gene="rplJ"
                     /locus_tag="VHH53_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX2289168.1"
                     /translation="MARPDKVDAVAEIADRFRTANAAVVTEYRGLSMTQLTSLRRSLG
                     ETTTYRVAKNSLVSRAAADAGVEGLDALFVGPTAIAFVQGEPVHAAKALRDFARENNA
                     LVIKGGYLDGRALTVAEVAQIADLESREVLLAKLAGAMQANLSKAARLFAEPASQVAR
                     LAAALRDKQQEQQPAGDSAED"
     gene            645..1034
                     /gene="rplL"
                     /locus_tag="VHH53_03015"
     CDS             645..1034
                     /gene="rplL"
                     /locus_tag="VHH53_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX2289169.1"
                     /translation="MAKLSTDELLDVFKDMTLLELSGFVKKFEETFDVTAAAPVAVAA
                     GPVGAAAEAPAAEEKDEFNVVLEAIGEKKIQVIKVVREVVSGLGLKEAKDLVESAPKP
                     LLEGVPKDQADAAKAKLEEAGAKVSLT"
     gene            1331..1918
                     /locus_tag="VHH53_03020"
     CDS             1331..1918
                     /locus_tag="VHH53_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289170.1"
                     /translation="MTSTAGIRHFLGGPLRIATLAAAVLAVIAFVVAGWFGVAWYRAA
                     HDDSLARGVARDAVLQDAQQTVINLNTVDHRQVQEGLTLWEQSAAGSLLGEVQANRDN
                     YVRAITDSKTTTTARVIDGAVAALNEGSGTARVLVGVDVTSQFDGGRASCVRRRIQLD
                     MHRDGDAWKIDKLVPVGDAIPLTGACPAPTPVPAK"
     gene            1940..2503
                     /locus_tag="VHH53_03025"
     CDS             1940..2503
                     /locus_tag="VHH53_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289171.1"
                     /translation="MTIPTRPQSDAPAEQVTKPPRRLTGVAALVGLVAALVIALGLAA
                     WFRGEANQLLGSAAAGNDALVDTEATAQVSEQVRDAVERIFSYDFAQLDANERAAAGV
                     ITGPLVQEYRQQFAQVRELAPPQQAVVATTVPALAVKMLDGDRAVVVVFIERQARQGP
                     QPPPMLGLTVTAQRVDGSWKIADVAPF"
BASE COUNT          480 a          892 c          910 g          431 t
ORIGIN      
        1 ggggccggtg caccacttcc cattgcccac gacaggaggc gatccatggc caggccggac
       61 aaggtcgacg ccgtcgccga gatcgcggac cggttccgca ctgccaacgc tgcagtcgtg
      121 accgagtacc gcggcctctc catgacgcaa ctgaccagcc tgcgccgttc cctcggcgag
      181 acaaccacct atcgggtcgc caagaacagt ctggtttcgc gcgcggcggc cgacgctggc
      241 gtggagggcc tggacgcgct gttcgtcggc ccgaccgcaa tcgcgttcgt ccaaggcgag
      301 ccggtgcacg cggccaaggc actgcgtgac ttcgccaggg aaaacaacgc actggtcatc
      361 aagggcggct acctcgacgg ccgggcgctg accgtcgcgg aggtcgccca gattgcggac
      421 ctggagtctc gtgaggtgct gctggccaag ctggccggcg cgatgcaggc caacctctcc
      481 aaggccgcca gactgttcgc cgagccggcc tcccaggtcg cccggttggc cgcggcgctg
      541 cgggacaagc agcaggagca gcaaccggcc ggtgacagcg ccgaagactg atcgagccta
      601 atgactatca agacagtgat tgtcaagaca aggagaatgc catcatggcg aagctgagca
      661 ccgacgagct gctcgatgtg ttcaaggaca tgacgctgct cgagctgagc ggtttcgtga
      721 agaagttcga ggaaaccttt gatgtcaccg ccgcggcgcc ggtcgcggtg gcggcgggtc
      781 cggtcggtgc agccgccgag gctccggccg ctgaggagaa ggacgagttc aacgtcgtcc
      841 tcgaggccat cggcgagaag aagatccagg tcatcaaggt ggtccgggag gtcgtctccg
      901 ggttgggtct caaggaggcc aaggacctcg tcgagagcgc acccaagccg ctgctggagg
      961 gcgtgccgaa ggaccaggcc gacgccgcca aggccaagct ggaagaggcc ggcgccaagg
     1021 tcagcctcac ctgagaacac cacacatcga cggccattcg cattcagcgg gctgcgcggg
     1081 ttatccggcc ttgccatgac cccgctcagc ccgctgaaac gcgttcgagc ccagcacacc
     1141 aggtcggtga gcacctcgtc gcgctcgagt tcgttgaaca cctcgtggta gaggtccggg
     1201 catggacgca cagtgtcgtg cttggtggcg taccgcaaca cgatcagccc gccgagccag
     1261 tggtcgacca tgaaccgggc cacgtggacc ctcacgccta cccaacgtca tctctctacc
     1321 ctggacggcc atgaccagca ccgcaggcat ccggcacttc ctgggcggac cgcttcgcat
     1381 cgccaccctt gcggctgcgg tgctcgccgt gatcgccttc gttgtcgccg gctggttcgg
     1441 cgtggcctgg taccgcgccg cgcatgatga ctcgctcgct cgaggtgtag cccgagatgc
     1501 tgtgcttcaa gatgcccagc agaccgtgat caacctcaac acggtcgatc atcggcaggt
     1561 gcaggagggg ctgacactgt gggagcagtc ggcggccgga tcgctgctgg gtgaggtgca
     1621 agccaatcga gacaactatg tgcgcgccat caccgactcg aaaacgacca ccaccgcaag
     1681 ggttatcgac ggagcggtcg ccgctctcaa tgagggttcc ggcactgcgc gggtgctcgt
     1741 cggggtcgac gtcacgtccc agttcgacgg tggcagagca agctgcgtgc gcaggcgcat
     1801 acaactcgac atgcaccgcg acggcgatgc ctggaagatc gacaagctgg taccggtcgg
     1861 tgacgccatc ccgttgaccg gagcgtgccc tgcgcccacg cccgtgcccg cgaaatagcg
     1921 aaggggactc ggagatcgca tgaccatccc gacccgccca caatccgatg cccctgccga
     1981 gcaggtgacc aagccgcccc ggcgtctgac cggggtggcg gcactggtgg ggctggtggc
     2041 tgcgttggtg atagcgctgg gtctggcggc atggttccgc ggcgaggcca accagctgct
     2101 cgggtctgcg gctgcgggca atgatgcatt ggtcgacacc gaagccaccg cccaggtctc
     2161 cgagcaggtg cgcgatgcgg tggaacgcat cttctcctac gacttcgccc agttggatgc
     2221 caacgagcgg gctgctgccg gcgtcataac cggtccgttg gtccaggagt accgccagca
     2281 attcgctcaa gtgcgggagt tggcgccgcc gcagcaggcc gtggtggcga cgaccgtgcc
     2341 cgcgctcgca gtcaagatgc tcgacggtga ccgcgcggtc gtagtggtct tcatcgagcg
     2401 gcaagccagg cagggccccc agccaccgcc catgctcggg cttactgtca ctgcgcagcg
     2461 agtcgacggc agctggaaga tcgccgacgt ggcgccgttc tgaccgcggc gaccttctgc
     2521 tggccattta cggtccaccg ggctcggtcc gaatcccgcc gataggcgga gccgatcgga
     2581 cttccagccg ctgatcgtgc gtatccgacc actgatcgga cgatgcactc ttgacgggcg
     2641 gcgtgccgca ggtcactcta ggagcacgct tgcaccgggt tgcgcccggt gctggacagt
     2701 cgccgtccta gcg
//
LOCUS       DATADN010000226         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_327942, whole genome shotgun sequence.
ACCESSION   DATADN010000226 DATADN010000000
VERSION     DATADN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327942"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VHH53_03030"
     CDS             complement(<1..362)
                     /locus_tag="VHH53_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2289172.1"
                     /translation="MNDPLIRAGIFQGVDPSAVQALSATLEPVKFPRAHVIFAEGEPG
                     DRLYIVLSGKVKIGRKSADGRENLLAVFGPSDMFGELSIFDPGPRTSTATTVTEVSAV
                     SMDRAALRDWINKRPEIA"
     gene            complement(444..716)
                     /locus_tag="VHH53_03035"
     CDS             complement(444..716)
                     /locus_tag="VHH53_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019855884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289173.1"
                     /translation="MTVWFVVAIPPLIMFFALAMERLETRLRHVAMHDNAAVQEKEVE
                     EFLQTARADEVRALFRSGIGRALELFQRRRLRRVALIRSRRPRQRS"
     gene            922..1674
                     /gene="nth"
                     /locus_tag="VHH53_03040"
     CDS             922..1674
                     /gene="nth"
                     /locus_tag="VHH53_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX2289174.1"
                     /translation="MNRVLAIAYPDAHCELDFSTPLQLAVATILSAQTTDKRVNETTP
                     ELFRRYHSAADYAAADRDELEELIRPTGFYRNKTTSLIGLGTALVERFDGELPSRLED
                     LITLPGIGRKTANVILGNAFGVPGIAVDTHVGRLVRRWGWTQHEDPVKVEQAIADLIP
                     QRDWTTLSHRVIFHGRRVCHSRRPACGVCPLAGDCPSYGIGPAEPAIAAVLLRGPEAP
                     HLLAMAGLPTDLAGSSAAVGEVATVAASEAVR"
     gene            1671..2348
                     /locus_tag="VHH53_03045"
     CDS             1671..2348
                     /locus_tag="VHH53_03045"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2289175.1"
                     /translation="MRPAVRWVMVTALLVFALAVALWPRADTPTATSAGAAQRAGAPA
                     NLDELRSRAALQPCPDATSSAIPAGGVLSGVELPCLGQPVMVDLGAALAGRPALLNLW
                     GPLCQPCAQELPALAAYAAGTDAVPVLGVEVQRLPEGALEMLTALNVHYPSVSDPDGR
                     LRAALGAPPVLPLSFVVSADGRVSQVNPPEVLRSPEQVRAVVQRYLDPGYREPGSQGP
                     GSHGTVR"
     gene            2345..>2884
                     /locus_tag="VHH53_03050"
     CDS             2345..>2884
                     /locus_tag="VHH53_03050"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX2289176.1"
                     /translation="MSTQPPMVTAGPLVDPASVPGWLGLLVRRTAELGPADLGYIPAP
                     RQATLTARRSAAVLVLFAEDSCTGEPDVLLELRSDQLSSHAGQVCFPGGAVDDADDGP
                     VATALREASEEVGVRAEDVRPVAVMPPLYLPPSDFVVTPVLGHWQRPGPVAPVDHRET
                     AAVARVLLRVLADPVNRLTV"
BASE COUNT          410 a          968 c         1015 g          491 t
ORIGIN      
        1 tcagcgatct ctggtcgctt attgatccag tcccgcaatg cagcacggtc catgctgact
       61 gcgctgacct cggtcaccgt ggtcgccgtc gaggtccgtg gtcccgggtc gaagatcgac
      121 agctccccga acatgtccga aggcccgaaa accgcaagca ggttctcccg gccatcggcc
      181 gacttccgtc cgatcttgac tttgcccgag agcacgatgt acaggcggtc ccccggctcg
      241 ccttcggcaa agatgacgtg ggcccgaggg aacttcaccg gctccagcgt ggcgctgaga
      301 gcctgaaccg ccgagggatc gacaccctgg aaaatgccgg ccctgatcag agggtcgttc
      361 accgcggtcc tccagttgcc cccggcggaa ccggcccctc cgtcagggga cctaccaggc
      421 gccgccgatc actgggatgg attctaggac cgttgccggg gacggcgtga cctgatcagc
      481 gcgacgcggc gtagccggcg ccgctggaac agttccaggg cgcggccgat cccgctacgg
      541 aacaacgctc gcacctcgtc agctctggcc gtctgcagga actcctcgac ctctttttcc
      601 tgcaccgcgg cgttgtcgtg catggcaaca tggcgcaggc gagtctccag gcgctccatt
      661 gcgagcgcga agaacatgat cagtggcggg atggcgacga cgaaccagac ggtcatggtg
      721 cgggtgatcc tgccgcatca cctgaagggg ttgggcgtgg ggtggcctgc ccggcgtgtc
      781 ggccccttca ccgggcatca tgctgcccgg cccgtgcgac cgggtccgcg cccacctccg
      841 taggctgtgg gggtgccagc ccgatcccgt cggtccatcg atggagagag ccgtctcgca
      901 ctggttcggc gagcccgccg gatgaaccgg gtactggcca tcgcctaccc ggatgcacac
      961 tgcgaactgg acttctccac gccgcttcag ctggcggtcg ccaccatcct gtctgcgcaa
     1021 accactgaca agcgggtcaa cgagaccacg ccagagttgt tccgccggta tcacagcgcc
     1081 gccgactacg cggctgccga tcgggacgag ctggaggagc tgatccggcc caccgggttc
     1141 taccggaaca agacgacatc cctgatcggg ctcggcactg cgctggtgga gcgcttcgac
     1201 ggcgagctgc ccagccgcct ggaagacctg atcacgctcc cgggaatcgg gcgcaagacc
     1261 gccaacgtga ttctgggtaa cgctttcgga gtgcccggta tcgcggtgga cacccacgtc
     1321 ggacggctgg tgcgccgatg gggatggacc cagcacgagg atccggtgaa ggtcgaacag
     1381 gcgatcgccg acctgattcc ccagcgggac tggacgacgc tgtcgcaccg cgtgatcttt
     1441 cacgggcgcc gggtttgcca ttcccgccgc ccggcatgcg gggtgtgccc gctggccggc
     1501 gactgcccgt cctacggcat cggacccgcc gaacccgcga tcgccgcggt gctgctgcgc
     1561 gggcctgagg ctccccacct gctggcgatg gccgggctgc ccaccgatct agcgggttcg
     1621 agcgctgcag tgggcgaggt agccacggtc gcggcttccg aggcggtgcg gtgaggcctg
     1681 cggtgcgctg ggtgatggtg acggcattgc tggtgtttgc actcgccgtg gcgttgtggc
     1741 cgcgcgcgga cactccgacc gctacttcgg ccggtgctgc gcaacgtgcc ggcgcgccgg
     1801 ccaacctcga cgagcttcgg tcccgggcgg ctctgcagcc ctgcccggac gcgacgtcaa
     1861 gtgccatccc ggctggcggg gtgctgtccg gagtcgagtt gccttgcctg gggcagccgg
     1921 taatggtcga cctcggggcg gcgctggctg ggcggcccgc gttgctcaac ctgtggggac
     1981 cgctgtgtca gccatgcgcc caggagctgc ccgcactggc cgcttacgcc gccgggacgg
     2041 acgcggtgcc ggtgctcggt gtggaggtgc agcggctgcc tgagggggcg ctggagatgc
     2101 tcaccgcctt gaacgtgcat tacccctcgg tcagcgatcc cgatggcagg ctgcgcgccg
     2161 cgctgggtgc ccctccggtg ttgccgctga gcttcgtggt gtccgccgac gggcgggtca
     2221 gccaggttaa cccgcccgag gtgctgcgca gcccggagca ggtgcgcgcg gtggtacagc
     2281 gttaccttga cccgggttac cgcgaacctg gcagccaagg ccctggtagc catggaacag
     2341 tgagatgagc acgcagccgc cgatggtgac ggctgggccg ctcgtcgacc ccgcctcggt
     2401 accggggtgg ctgggcctgt tggtacgtcg caccgcagag ctcgggcctg ccgatctcgg
     2461 gtacattccc gccccacggc aggccacgct gacagcccgc cggtcggcgg cggtgctggt
     2521 gctttttgcc gaagactcgt gcaccggtga gccggatgtg ctgcttgagt tacgttccga
     2581 ccagttgtcc tcgcatgccg gccaggtctg cttccccggt ggagcggtgg acgacgccga
     2641 tgacggaccg gttgcgacgg cgctgcgtga ggcctccgaa gaggtcggcg ttcgcgccga
     2701 agacgtgcgg ccggttgcgg tgatgccacc gctgtacctg ccgccgtcgg atttcgttgt
     2761 tacgccggtg ctaggtcatt ggcaacggcc cggtccggtg gcgccggtgg atcacaggga
     2821 gaccgctgcg gtcgcccggg tgctgctgcg agtgctcgcg gacccggtga accggctgac
     2881 tgtg
//
LOCUS       DATADN010000227         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_931255, whole genome shotgun sequence.
ACCESSION   DATADN010000227 DATADN010000000
VERSION     DATADN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931255"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2614)
                     /locus_tag="VHH53_03055"
     CDS             complement(<1..2614)
                     /locus_tag="VHH53_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HEX2289177.1"
                     /translation="MTWQAGALAGLALLALVSAIGVLLAPVIVDDPVVSWPRAGEPPR
                     STVLPLVPYRPLSLDATVPCAALSALAQRAGGGDALRTQPPSTITPDDLDIPVVLSKR
                     AKKDPSDEISAGLVVAAHRGMVQITASGTTVLRETLVSGRCTYRIMADAGGVRVLRDG
                     EQRAATAVPVPEVGELATDLEGQPAAQGLAVSVHTDARYESRPTTLKIVLLVLHGVAL
                     LVLLGLAWRWGGGTAGRTQAPAVRRRRIRAADAVLVAVSALWVLLAPTNFDDPWYLLM
                     ARGGDISGSIGNAIYMFNVTENPFVASQYVLQAWASLGQSFGAAWGLAWMRLVPLIYG
                     LLTWVLLRTLLVKGFGSELGTGKAVWALLVAYLLWWLPYGMTLRPEPLIVLLTAATLL
                     LAELARQRRSVGALAAATAIAALAVSVSPSGLVAMAPLVLALPWLWAWLREQRVVARV
                     AAVALLAAAGTSLVPVGFADATLGDFLESAAVHQWYYDTFAWFEELAHYRTILSLQDS
                     SQWARRAPVVLTVAVLLVVALSSVVRRKLSGPHLRTEVDPVRRLLLSSAACSAVALAL
                     LAPTPTKWVNHFGAAAAAPIVLLAAALLCRPLGPPLGFPLHRDLRTGAAIAAAAVLVG
                     AVSLSFAGPNLWRPYSDRGQPFGDHIAAVATASPDLGSMRLRLGPVQLANPVLWIAVA
                     LAAGCWVWWRYRRGHVAGLTPDRAVLLTGSTTLVVMTIVVFVWAPLRQYPGWSVAMSA
                     ARSMSGEPCVLARHAQVLVDTAVQPVPLGPAIVEGSFAAAAEWPLPVPAPASGTLVWH
                     NAVPAGPQTGLLVTPWFALPAHRSTDDGTHLLVPLLGARAGQMLILEYATAAGPNPAM
                     ARSGLPVARRCRPPR"
BASE COUNT          466 a          961 c          923 g          325 t
ORIGIN      
        1 cccgcggtgg ccggcagcgt cgcgctaccg gtagacctga ccgtgccatc gcggggttcg
       61 gcccggccgc ggtggcgtac tccaagatca gcatctggcc ggcccgggcg ccgagcaatg
      121 ggaccagcag gtgggtgccg tcatcggttg atctgtgcgc gggcagcgcg aaccacggtg
      181 tcaccagcag ccccgtctgg gggccggccg ggacggcgtt gtgccagacc agtgtgcccg
      241 aagccggggc aggcaccggt aatggccact ccgctgctgc ggcgaacgac ccctcaacga
      301 tcgccggacc cagcggtacc ggctgcaccg cggtatcgac gaggacctga gcgtgcctgg
      361 ccagcacgca tggctcgccg gacatgctgc gagcagccga catcgcgacc gaccagccgg
      421 ggtactgccg taacggagcc cacacgaaga cgacgatggt catcaccacg agggtggtcg
      481 agccggtcag caatacagcg cggtcgggtg tgagcccggc gacgtgacct cgccggtacc
      541 gccaccacac ccagcagccc gcggcgagcg cgaccgcgat ccacagcacc gggttggcca
      601 gctgcaccgg tcccagccgc agtcgcatgc tcccgaggtc gggcgaggct gtcgccaccg
      661 cggcgatgtg gtcgccgaag ggttggcccc ggtcggagta gggacgccac aggttcggtc
      721 cggcaaagct cagcgacacc gcacccacca gtaccgccgc tgccgcgatg gccgcgccgg
      781 tgcgaagatc gcggtgcagc gggaagccca gtggcggtcc cagcggccgg cacagcaaag
      841 ctgcggcgag cagcactatt ggcgcagcag cggcggcccc aaaatggttg acccacttgg
      901 tgggtgtcgg tgcgagcagc gccagggcca ccgccgaaca ggccgcactg ctcaacagca
      961 gccggcgcac tgggtcgacc tcggtacgca ggtgtggacc gctcagcttc ctgcgcacca
     1021 cgctgctcag cgcgaccacc aggagcaccg cgacggtcag cacgacagga gcccgccggg
     1081 cccactggct ggagtcctgc aggctgagga tcgtccggta atgggcgagt tcctcaaacc
     1141 aggcaaaggt gtcgtagtac cactggtgca ccgcggcgga ctccaggaag tcgcccaggg
     1201 tggcgtcggc gaatcccacc ggcaccaggc tggtacccgc ggctgccagc aacgccaccg
     1261 cggccacccg cgccacgacc cgctgctcgc gcaaccaggc ccatagccag ggcagtgcca
     1321 gcaccagcgg cgccatcgcc accagcccgg aaggggagac cgacaccgcc agcgcggcga
     1381 tcgcggtggc tgcggccagc gcgccgaccg agcggcgctg ccgggccagc tcggccagca
     1441 acaatgtcgc ggcggtgagc agcacgatca gcggttccgg tcgcagtgtc atcccgtagg
     1501 gcagccacca cagcaggtag gccaccagca gcgcccagac agccttgccg gtacccagct
     1561 cggaaccgaa tcccttgacc agaagagtgc gcagcagcac ccaggtgagt agcccgtaga
     1621 tcagcggcac caaccgcatc cacgccagcc cccaggccgc cccaaatgat tggcccaagc
     1681 tggcccatgc ctgcagcacg tactggctgg ccacgaaggg gttctcggtg acgttgaaca
     1741 tgtagatcgc gttgccgatc gagccggaga tgtcgccacc gcgggccatc agcaggtacc
     1801 acgggtcatc gaaattcgtc ggggccagca gtacccacag cgccgagacc gccaccagca
     1861 ccgcgtcggc ggcccggatc cggcggcgcc gcaccgcagg cgcctgggtc cggccggccg
     1921 taccgccccc ccaccgccaa gccaacccga gcaggaccag cagggcgacg ccgtgcagca
     1981 ccagtagaac gatcttcagt gtggtcggcc tggactcgta acgggcatcg gtgtgcaccg
     2041 acactgccag gccttgggcg gccggttggc cctcgaggtc agtggccagc tcgccgacct
     2101 cgggcactgg cacggcagta gcggcgcgtt gctcaccgtc gcgcagcacc cgtaccccgc
     2161 cggcgtcggc catgatgcga taggtgcacc tgccagagac cagtgtctcc cgaagcaccg
     2221 tggtgcccga cgccgtgatc tgcaccatgc cgcggtgtgc cgcgacgacc aggcccgcgc
     2281 tgatctcgtc tgaagggtct ttcttcgcgc gtttgctcag tacgaccggg atatccaggt
     2341 cgtccggtgt gatggtgctg gggggctggg tgcgcagcgc gtcgccgcca ccagcccgct
     2401 gggccagggc ggacaacgca gcgcatggca ccgtggcgtc caagctcaac gggcggtaag
     2461 gcaccagcgg tagcacggtg ctgcggggcg gttccccggc gcggggccag ctgaccaccg
     2521 ggtcgtcgac gatcaccgga gcgagcagca cgccgatcgc cgagacgagg gcgagcaaag
     2581 ccagcccggc cagtgccccc gcctgccagg tcaccgcctg ccgcgccgtg gtccgttccg
     2641 aacgggacgc agcggccacg gcggggagaa taacg
//
LOCUS       DATADN010000228         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1381326, whole genome shotgun sequence.
ACCESSION   DATADN010000228 DATADN010000000
VERSION     DATADN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381326"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VHH53_03060"
     CDS             complement(<1..473)
                     /locus_tag="VHH53_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2289178.1"
                     /translation="MTVNTEFVRLEVDGGIGMIRLDRPPMNALSRRCADEIRSAAREA
                     ADRQDVRAVIVYGGEKVFAAGADIKEMAAMPASEMAAQAQELSSSFAAVAAIPKPTVA
                     AITGFALGGGYELALCCDRRIAADNAKVGQPEILLGIIPGAGGTQRLPRLIGPARA"
     gene            complement(485..1249)
                     /locus_tag="VHH53_03065"
     CDS             complement(485..1249)
                     /locus_tag="VHH53_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2289179.1"
                     /translation="MDGVGVRRGATALLDRVDWRVELDEKWVILGPNGAGKTTLLRLA
                     ATELHPTCGTVHVLGELLGRTDVFELRPRIGFSSASLASRVPGEEVVSDLVVSAGYAV
                     LGRWRESYADLDNARATGLLAALGVVELAGRRFGTLSEGERKRVLIARALMTDPELLL
                     LDEPAAGLDLGGREDLLARLATLAADPDAPATVLVTHHVEEIPPGFTHAMLMRDGAVV
                     AQGLLDSVLTEENLVKTFDVDLVLRRSGDRFFAYRR"
     gene            1361..>1764
                     /locus_tag="VHH53_03070"
     CDS             1361..>1764
                     /locus_tag="VHH53_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289180.1"
                     /translation="MTRLPPPQVGRPFPLGAHAEGGGVRFAVASAVAESLEVCLLSPG
                     GAELRVPLAERTFGVWHGLLPGVGPGQRYGYRVHGPWDPARGLRCNPTKLLVDPPRVR
                     VDQQLGRVAPQPACGVPGPMYPIAVALPGSYAG"
BASE COUNT          274 a          641 c          579 g          270 t
ORIGIN      
        1 gcccgggccg gaccgatcag ccgcggcaac cgctgggtgc cgccggcgcc aggaataatg
       61 cccagcagga tctcgggctg gccaaccttg gcgttgtccg cggcgattcg ccggtcacag
      121 cacagcgcga gctcgtagcc accgcccaac gcgaagcccg tgatcgcagc cacggtcggt
      181 ttcgggatgg ccgccaccgc cgcgaacgac gaggacagtt cttgggcctg cgccgccatc
      241 tccgaggctg gcatcgcggc catctccttg atgtcggcac cggcagcgaa cactttctcc
      301 ccgccgtaaa cgatcaccgc gcggacgtct tgtcggtctg cggcctcccg cgcagcggac
      361 cggatttcgt cggcgcacct gcggctcaag gcgttcatcg ggggccggtc gagccggatc
      421 atgccgatgc cgccatccac ctcaagcctg acgaactcgg tgtttaccgt catgccttcg
      481 cacgctaccg gcggtaggcg aaaaaccgat ccccggaacg tcgcagcacg aggtcgacgt
      541 cgaatgtctt caccaggttc tcctcggtaa gcaccgaatc gagcaggccc tgggcgacca
      601 ctgccccgtc gcgcatcagc atggcgtgcg tgaagccagg cgggatctct tcgacatggt
      661 gagtcaccag cacggtcgcg ggcgcatccg gatcggcggc cagcgtggcc agccgagcca
      721 gcagatcctc ccggccaccg aggtctagcc cggcggcggg ctcgtccagc agcagcagct
      781 caggatcggt catcagcgcc cgggcgatca ggacccgctt gcgttcccct tccgacaggg
      841 ttccgaagcg ccgtccggcc agctcgacga cgccgagcgc tgccagcagt ccggtcgccc
      901 gggcgttgtc cagatcggcg taactttccc gccaccggcc gagcaccgcg taaccggcgc
      961 tgaccaccag gtcagacacc acctcctcac cgggcactcg gctagccagc gaggccgagg
     1021 agaaaccgat ccgcggacgg agctcgaaca cgtcggtgcg gcccaggagt tcccccagta
     1081 cgtggaccgt cccacaggtc ggatgcagtt cagttgcggc cagccgcagc aaggtggtct
     1141 tgccggcgcc attgggtccc agaatcaccc acttctcgtc cagctccacc cgccagtcca
     1201 cccgatcgag cagagccgtg gcgccgcgcc gcacgcctac tccgtccatc cacaccacga
     1261 gatccgtgag atcggccgag aagtgcccgt tctgcgcgtc caccccacac attgtgctgg
     1321 ggccgggtgc atgccgtacc gggcaggcag gattggtgtt gtgacccgac tgccacctcc
     1381 tcaggtcggc cggccgttcc cgcttggtgc gcatgccgag ggtggggggg tgcgcttcgc
     1441 ggtggcctcc gcggtcgccg agtccctcga ggtgtgcctg ctgtcgccag gcggcgcgga
     1501 gctacgggtg ccgctggccg aacgaacctt cggggtatgg cacgggctgc tgcccggcgt
     1561 aggacccggg cagcgctacg gctatcgggt acatgggccc tgggaccccg cacgcggcct
     1621 gcggtgcaac ccgaccaagc tgctggtcga tccgcctcgc gtacgggtcg accagcagct
     1681 tggtcgggtt gcaccgcagc ccgcgtgcgg ggtcccaggg cccatgtacc cgatagccgt
     1741 agcgctgccc gggtcctacg ccgg
//
LOCUS       DATADN010000229         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_780211, whole genome shotgun sequence.
ACCESSION   DATADN010000229 DATADN010000000
VERSION     DATADN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780211"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VHH53_03075"
     CDS             complement(<1..310)
                     /locus_tag="VHH53_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2289181.1"
                     /translation="MRIAFVGKGGSGKTTLSALFTRHLAELGRPVLAVDADINQHLGE
                     ALGLEDEQVPPLGGQLPWIKEHLRGSNPRITSADSMIKTTPPGRGSRLLRLDPDEELH
                     R"
     gene            517..1425
                     /locus_tag="VHH53_03080"
     CDS             517..1425
                     /locus_tag="VHH53_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2289182.1"
                     /translation="MTADLYRIDAEPSAPAGTVLVHALTGFVDAGQAGQVAVAHLLAN
                     LEHRVLATFDVDQLLDYRSRRPTMVFDGDRWESYERPELVLHEVRDSEGTTFLLLAGP
                     EPDLQWERFTDAVRELVERFGVGLTVGLGAVPMAVPHTRPVTVTAHATRRELISDYEP
                     WFGAMEIPGHAGALMELKLGQAGHDAMGFVVHVPHYLARIEYPESARGLLEHLARSSG
                     LSLPVEALRPAALRVLAEVDKQVAGSQESMSVVAGLEARFDAIVDGSDQRPLLSSDAG
                     PLPTGDEIAAELEQFLAEQDGGSAES"
     gene            complement(1422..>1714)
                     /locus_tag="VHH53_03085"
     CDS             complement(1422..>1714)
                     /locus_tag="VHH53_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2289183.1"
                     /translation="IDGGVRSATNADLAAGYERVVVLAPITRAFGPAMGAAKHVAALR
                     KQARATLVIPDADARRAIGRNLLDLARRAPAAQAGRAQATSAIVDVARVWSG"
BASE COUNT          290 a          548 c          581 g          295 t
ORIGIN      
        1 ggcgatgcag ctcctcgtcc gggtctaaac ggagcagccg ggaacctcgg cctggcggtg
       61 ttgtcttgat catcgaatcg gccgacgtga tccgaggatt gctgccgcgc aagtgttcct
      121 tgatccacgg cagctgccca cccaacggtg ggacctgctc atcctccaaa cccagcgctt
      181 cacccaggtg ctggttgatg tcggcgtcaa cggccagcac gggccgaccg agctcggcaa
      241 ggtgacgggt gaacagggcc gacaacgtcg tcttaccgct gccacccttg ccgacgaagg
      301 caatgcgcac tgtgctccga tctccttcca acgtggttga ccagatcgta aacgatagtc
      361 gttaccacta aagggtggcg caccgtctcc cggtcgtggc gcggagtgca cagcggtgtg
      421 ccgctgcagg cggatggatt cgacgctgtt cggtgttgta ccggttgcac ggcctgcatg
      481 atgagggccg accgagccga agcgaggtga cagcaagtga ccgccgacct gtaccgaatc
      541 gacgcggagc catcggctcc ggcggggacg gtactggtgc atgccctcac cggattcgtc
      601 gacgccggtc aagccgggca ggtggcagtg gcacatctgc tggcgaatct ggagcaccgt
      661 gtcctcgcca catttgacgt cgaccaactg ctggactacc gcagtcgtcg accgacgatg
      721 gtcttcgacg gggaccgttg ggaaagctat gagcggccgg agttggtcct gcatgaggtg
      781 cgtgacagcg aaggcacgac gttcttacta cttgctggcc cagagccgga tctgcagtgg
      841 gagcggttca ccgacgcggt ccgggaactg gtcgagcgct tcggcgtagg cctgactgtt
      901 ggcctgggtg cagtgccgat ggccgtccca cacacccggc cagtgacggt caccgcgcac
      961 gccacccgac gggagttgat ctctgattac gaaccgtggt tcggtgccat ggagatcccc
     1021 ggccacgccg gtgcgctgat ggagctgaag ctgggccaag ccgggcacga cgcgatgggc
     1081 ttcgtcgtgc acgtgccgca ctacctggcc cgcatcgagt acccagagtc ggcccgcggg
     1141 ctgctggagc atctggcccg cagcagcggg ctgagcctgc cggtggaggc gctacgccca
     1201 gcggcactgc gagttctagc cgaggtggac aagcaggtcg ccggttccca ggaaagcatg
     1261 tcggtggtag ccggcctgga ggcgcggttc gacgctatcg tcgatggatc tgatcaacgt
     1321 ccgctgctgt ccagcgatgc cgggcccttg cccactggcg acgagatcgc ggcggaactc
     1381 gagcagttcc tcgccgaaca agatggtggc agcgccgaga gctaacccga ccaaactctg
     1441 gccacgtcca cgatggccga cgtggcctgg gcgcgtccag cctgcgcggc gggcgctcgt
     1501 cgggccaggt ccagcaggtt gcggccgata gcgcgtcgtg cgtcggcgtc cggtatgacg
     1561 agcgtggccc gtgcctgttt gcgcaacgcg gcgacgtgct ttgccgcacc catcgccggc
     1621 ccgaaagcac gcgtgatcgg ggctagcacc acgacgcgct cgtaccctgc tgccagatcg
     1681 gcattggttg ccgagcgcac accgccatca atcc
//
LOCUS       DATADN010000230         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_477486, whole genome shotgun sequence.
ACCESSION   DATADN010000230 DATADN010000000
VERSION     DATADN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477486"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VHH53_03090"
     CDS             complement(<1..762)
                     /locus_tag="VHH53_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEX2289184.1"
                     /translation="MASKPRAGRLDSAPVREYPVPEDPAPEYRLYFVEVARSERLGPS
                     FVRVTFTGECLRAFGAAGVDQRIKLLLPQPGRTVAGIPSGPGWYPAWQAMPQEIRPTM
                     RTYTVRTHRPEVAELDIDFVLHGTAGGHGGPASAWAAKVRPGDTAALVGPDRPGSGRM
                     WGCAWSPPDTARHLIIAGDETAVPAVASIIESLPPQARGVVCVEVPTAGDRQDWDVPD
                     RVEVRWLVRRDSTGAAPHGSLLEAAVTEVLTELCGP"
     gene            853..1179
                     /locus_tag="VHH53_03095"
     CDS             853..1179
                     /locus_tag="VHH53_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX2289185.1"
                     /translation="MTCPKCHGSLRTVDKQGVRLEQCENCRGIFLDHGELEQLLRAEQ
                     HYYGGGVPPYAGHSGGHRGHSDSPRGYRDSPRPYRGDHHGRYADSPRPYGHQRSHGSF
                     LGGLFG"
     gene            1278..>1545
                     /locus_tag="VHH53_03100"
     CDS             1278..>1545
                     /locus_tag="VHH53_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HEX2289186.1"
                     /translation="MARQPLFCLAGPSGTGKSTVARALLPRLSDRFVVLEQDVLWLPE
                     LSDPTDEHRLFRSTWLRLAAMIGCSGRPVLLCGTVVPPELEPLPE"
BASE COUNT          232 a          533 c          515 g          265 t
ORIGIN      
        1 cggcccgcac agttcggtca ggacctccgt cacggctgcc tcgagcaacg acccgtgcgg
       61 ggccgctccg gtgctatctc ggcgtaccag ccaccgcacc tcgacccggt cgggaacgtc
      121 ccagtcctgc cggtcgcctg ccgtcggcac ttcgacgcag acgacgccac gggcctgcgg
      181 cgggagcgac tcgatgatcg acgccaccgc aggaacagcc gtctcgtcac cggcgatgat
      241 caggtgtctg gccgtgtctg gcggggacca cgcgcacccc cacatccgac cgctacccgg
      301 ccgatccgga ccgaccagcg cggcggtgtc accgggacgg accttcgcgg cccaggccga
      361 ggcagggcct ccgtggccgc cggctgtgcc gtgcagcacg aagtcgatgt ccagctcggc
      421 cacctcgggc cggtgtgtcc gcacggtgta ggtgcgcatg gtcggacgta tctcttgcgg
      481 catcgcttgc catgccgggt accagccggg tccgctggga atgccagcca ccgtccggcc
      541 aggctgcggc agcaagagct tgatccgctg atcgacgcca gcagcgccga aagctcgcag
      601 gcactctccg gtgaacgtga ctcgtacgaa actgggtccg agccgctcgc tgcgagccac
      661 ctcgacgaaa tacaggcggt attccggcgc tgggtcttcc ggaactgggt attcgcgcac
      721 tggcgcgctg tcgagtcgcc cagccctggg tttcgatgcc atcccgcctc cccttcttag
      781 gtgaggctac ctatattcag gcggcgctga cgcgctgtgc gggccgaaaa cgacggatcg
      841 tatgctgatt gagtgacatg tccgaaatgc catgggtccc tgcgaaccgt cgacaagcaa
      901 ggggtgcgcc tggagcagtg cgagaactgc cggggcatct tcctcgatca cggcgagctg
      961 gaacagctcc tgcgggccga gcagcactac tacggcggtg gggtgccgcc gtatgcgggg
     1021 cattcgggtg ggcatcgcgg ccactcggat tcgcccaggg gttaccggga ctctccccgg
     1081 ccctaccggg gagatcacca cgggcgctac gctgactcgc cgcgtcccta cggacatcag
     1141 cgcagccacg gaagcttcct cggcgggctg ttcggctaga ccgcgcgcgg taccggatga
     1201 acccacggat ctgcgaccgc tgtggatgct tgctagcacg cccgctgatc tgcggcgagt
     1261 gcggccacca gcagtccttg gcccgccaac ccctgttctg cctggcaggc cccagcggta
     1321 ccggtaagtc cactgtggcc cgtgctctgc tgccgcggct gtctgatcgt ttcgtggttc
     1381 tcgaacagga tgtgttgtgg cttcctgagc tcagcgatcc caccgatgag catcggctgt
     1441 tccgttcgac gtggctgcgg ctggccgcga tgatcggatg cagcgggcgg cccgtgctgc
     1501 tgtgcggcac cgtggtgcct cccgagctgg aaccgttgcc cgaac
//
LOCUS       DATADN010000231         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_931946, whole genome shotgun sequence.
ACCESSION   DATADN010000231 DATADN010000000
VERSION     DATADN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931946"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VHH53_03105"
     CDS             complement(<1..438)
                     /locus_tag="VHH53_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEX2289187.1"
                     /translation="MDVGTIAALVAGAGLVGIVVWALAKIGHALIKVAEALAAVAVVV
                     FAVWLMIKLGMWAIRQAFIRWRTSLTVLGVAAWWHWWGWPSLALIAGAIAAVLIGWRV
                     INVVSFDAWAGRHLRAWWLRWTVYAPKLPAWLNACGLGIKHDAA"
     gene            complement(632..826)
                     /locus_tag="VHH53_03110"
     CDS             complement(632..826)
                     /locus_tag="VHH53_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289188.1"
                     /translation="MSALAISDITEREATYRRGEQLVRHAAEAERVAFVHWQEAYGAY
                     EVACDEASVAWTAWIEAVTP"
     gene            complement(823..1221)
                     /locus_tag="VHH53_03115"
     CDS             complement(823..1221)
                     /locus_tag="VHH53_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289189.1"
                     /translation="MRVKRPAITDLEEQQTMQLEIKTEGVEFVVSRAPQPKNDNDGRQ
                     KADRDTGAPLHVTELVAMDETGAEVIKVTTAGEPRVTKRQLVTVVKLVATPWNIDGRG
                     GVAFRADGIVPVPTTPAAHRASAAAAAGGS"
     gene            complement(1546..>3292)
                     /locus_tag="VHH53_03120"
     CDS             complement(1546..>3292)
                     /locus_tag="VHH53_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX2289190.1"
                     /translation="GQYVYWIEGRPSGDVLVRACEPGADDVLPPGVAVASYVHEYGGG
                     AYLATDDAVWFVRADDQQIWRITRTGLRAVTAAPQHGEDRYADLRLCAPGLLVAVRER
                     HHNGAVTNELVMVPADGSAAPWPVAQGWDFYSFPRPSPDGRALAWTTWRHPLMPWDGT
                     WLWVAEIRKDGTLGAARHIAGGPEESVFQPEWSSGGVLHFVSDRTGWWDLYACTPRGD
                     IVSLIHLNSTELATAQWEFGYSTYAFLDNDRIAVLAHQGGVTQLNIWDRRRSEVHAVE
                     LPYTSIKPYLAAHATQVALIGGSPDRMPAVCVIDVDSGQSRELTATAGVAPGWQAPNP
                     EVVEILARDGGRVYATLHRLSPDKGSAPAPVVVRAHPGPTANAPLRLDPWVQFFVDHG
                     FAVLDVDYRGSTGYGRAYRNALRGHWGVLDVANCVDAVQALGQGGILDLTRVVITGAS
                     AGGYTALRAATTTKNFRAATVRSAIVDPAAWRAAAPKFQSHHADLLLGPPATWRDRSL
                     LRPGTTTHCPILAFHGHQDGITPLDQVQQFAAMAGEQASLVVYPNEGHGLSRPENIEH
                     AIYAELAHYRAALDS"
BASE COUNT          590 a         1108 c         1026 g          568 t
ORIGIN      
        1 cgcggcgtcg tgcttgatgc ctaggccaca ggcgttcagc cacgcgggca gtttgggggc
       61 gtacaccgtc cagcgcagcc accaggcccg cagatgccga cccgcccagg catcgaacga
      121 caccacattg atcacgcgcc agccaatcag taccgccgcg atggcccctg cgatcagggc
      181 cagcgacggc cagccccacc agtgccacca ggccgccacc cctagcacgg tcaggctggt
      241 ccgccagcgt ataaatgcct gccggatggc ccacatccca agcttgatca tcagccacac
      301 ggcgaagacc actaccgcta ccgcggccag cgcttcggcg accttgatca gtgcgtggcc
      361 gattttggcc agggcccaca ccacgatgcc caccagcccg gcaccggcga ccagcgccgc
      421 aatcgtgcca acgtccatca gcgaccaccg ccgcagcact gttgaccact cgcgccggca
      481 gtgcgggcgc tgatcagtcg ctggtgcacg catttttggt cgtgctcgtc tcgccgtagg
      541 ctcatgacag ctcctcttgt cctgaagtcg atgggtggag cggggcgccc ggccggcctt
      601 ggtttccagg cccgaccggc cgggcgcgca cctaaggggt gacggcctct atccaggccg
      661 tccaggcgac ggacgcctcg tcacaggcga cttcgtaggc gccgtaggcc tcctgccagt
      721 gcacgaacgc gacacgctct gcctcagccg cgtgtcggac gagctgttca cctcgccggt
      781 aggtggcttc gcgctcggtt atgtcgctga tcgctaaggc gctcatgagc cacctgccgc
      841 tgcagcagcg ctggccctgt gggcagccgg tgtggtcggt accgggacga tcccgtccgc
      901 ccggaatgcc accccgcctc ggccgtcgat gttccacggc gtcgcgacta gcttcaccac
      961 cgtgaccaac tgccgcttag taacccgtgg ctccccggcg gtggtgacct tgatgacctc
     1021 cgcgcctgtc tcgtccatcg ccaccagctc ggtcacatgc agcggtgcac cggtgtcccg
     1081 gtctgccttc tggcggccgt cgttgtcgtt tttgggctgc ggtgcccgcg agaccacaaa
     1141 ctccacaccc tcggtcttga tttccaactg cattgtttgc tgttcctcca gatcggtgat
     1201 ggcgggccgt ttgacccgca caccaatcaa acgacgaaac tcatgcgccg aggtagtccg
     1261 cgaactagtc ccgaactagt ctgcttctag tcccgaccgc ccaactccgt gagccaccac
     1321 ttaacgcgga ggatcgtgac gccggcagca atcagagggg tgttcttgaa atgcttcctg
     1381 acgtggcaag gccacgcaat atcatcatca ggtcatagct cggtgttgag cagagggtcg
     1441 ttggcagcca cgatccgcac tgtcgtgagt gtgcgcgggg ccagcgggtc cctaaatgtg
     1501 ttaagcctca tcagcgccga gcaaagaagg gcagtgcgcc agcactcagc tgtccagcgc
     1561 tgcgcgatag tgcgctagct cggcatagat cgcgtgttca atgttctcgg gtcggctcag
     1621 tccgtgaccc tcgtttggat agaccacgag cgatgcctgc tcaccggcca tggcggcaaa
     1681 ctgctggact tggtcaagtg gcgtgatccc gtcctggtga ccgtggaagg cgaggatggg
     1741 gcagtgggta gttgtaccgg ggcgcagcag ggaccggtcg cgccaagtag cgggcgggcc
     1801 caggagaagg tcggcatgat gcgattggaa cttcggggcg gcagcgcgcc aagcagccgg
     1861 gtccacaatg gcggaacgga cggtcgctgc ccggaaattc ttcgtggtcg tcgcggcacg
     1921 caatgcggtg taaccacctg cgcttgcccc ggtaatgacg acgcgagtca ggtcaagaat
     1981 cccgccttgt ccgagcgctt ggacggcatc gacgcagttg gccacgtcca atacacccca
     2041 gtgaccgcga agcgcgttgc ggtaagcgcg gccatagccg gtgcttcccc ggtagtccac
     2101 gtcgagtacc gcgaacccgt ggtcgacgaa gaactggacc caggggtcga gtcgtaacgg
     2161 tgcgttggcg gtgggccctg ggtgtgcccg gaccaccacg ggcgctggcg cggagccttt
     2221 gtccggtgag agacggtgca acgtggcata cacccggccg ccgtcccgag cgaggatctc
     2281 gacaacctcc gggttcggcg cttgccatcc cggcgcgact cccgcagtcg ctgtcaactc
     2341 gcgggactgg ccgctgtcga cgtcgatcac gcacacggcg ggcattcgat ccggggaccc
     2401 cccgattagc gccacctgtg ttgcgtgtgc tgccaaatac ggctttattg acgtataggg
     2461 caactcgacg gcgtgcacct cggaccggcg acgatcccaa atattgagct gggtgactcc
     2521 tccctggtgt gcgagcacag cgatccggtc gttgtccaga aaggcgtagg tggagtaccc
     2581 aaattcccat tgcgctgttg ccagctctgt tgaattaagg tggatcagcg agacgatgtc
     2641 accacggggt gtgcaggcgt acagatccca ccaaccggtg cggtcagaca cgaagtgcaa
     2701 gacaccaccg gaactccatt ccggctggaa cactgattcc tccggaccgc cggcaatatg
     2761 ccgagcagcg ccgagcgtgc catccttgcg aatctcggcc acccacagcc aggtgccgtc
     2821 ccagggcatc agcgggtgcc gccaagtcgt ccaggccagc gcacgtccgt cggggcttgg
     2881 ccggggaaac gagtagaagt cccagccctg tgccaccggc cagggggcgg ccgacccgtc
     2941 ggcgggaacc atcaccagct cgttggtcac cgcgccgttg tgatgacgct cccgtacggc
     3001 gacaagcagc cccggtgcac acaggcgaag gtctgcataa cggtcttcac cgtgctgggg
     3061 tgccgcagtc accgcgcgca acccagtccg agtgatcctc caaatttgct ggtcgtcagc
     3121 acgaacaaac cacacggcat catcggtggc caggtaggcg ccaccaccgt actcatggac
     3181 atacgacgcg accgccacac caggcggcag gacatcgtca gcgcccggtt cgcaggctcg
     3241 gaccagcacg tcaccactgg gtcgtccctc gatccaatac acgtactgcc cg
//
LOCUS       DATADN010000232         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1082630, whole genome shotgun sequence.
ACCESSION   DATADN010000232 DATADN010000000
VERSION     DATADN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082630"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VHH53_03125"
     CDS             complement(<1..706)
                     /locus_tag="VHH53_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX2289191.1"
                     /translation="MPVATALPTTSAHLSGLLEAVLAEPSLRALVEGARSPEMQLLGP
                     PATRPLAVAALARAGHPVLAVTATDREAGELTAEVADVLGAGVVAELPSWETLPHERL
                     SPRADTVGRRLAVLRRLAHPEEGITGSTGPLQVVVATVRSLIQPMLGGLGEIAPVRLR
                     VGQVHDFNDVLARLADLAYTRVDMVDRRGEFAVRGGIVDVFPPTADHPLRVEFWGDEV
                     CEIRSFAVADQRTLAPA"
     gene            1050..1745
                     /locus_tag="VHH53_03130"
     CDS             1050..1745
                     /locus_tag="VHH53_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2289192.1"
                     /translation="MDAFGPSGGRDMTSIVLGDDHVVFVESLVSVLVGAGFRVPAVGR
                     SLAGTIEAVRHHRPDVCLLSRHFSDGDGVTAIGPILAVSPATRILVLTAEGDTDAMRR
                     AVRLGAAGYVDKTWGVRKLVQALQRVVNGAVVLDAPRATTARSDISEAQRLAGHLTAR
                     ERECLALLVEGLDTRAMTLRLGVSITTVRSHVQALLTKLGVHSRLEAATFAVRYDLVD
                     HNQHGPDGRIKTG"
BASE COUNT          299 a          584 c          602 g          263 t
ORIGIN      
        1 cggcgggcgc cagggtgcgc tggtccgcga cagcgaacga gcggatctcg cagacctcgt
       61 caccccagaa ctccacccgc agcggatggt cggcagtggg cgggaagacg tcgacgatgc
      121 cgccccgcac cgcaaactca ccgcgccggt ccaccatgtc gacccgggtg taagcgaggt
      181 cggccagccg ggccagcacg tcgttgaagt cgtgcacctg cccgacccgc aaccgcaccg
      241 gcgcgatctc gccgagccca ccgagcatcg gctggatcag cgaccgcacg gtggccacca
      301 cgacctgcag cggcccggtg gagccggtaa tgccttcttc cgggtgggcc agccggcgca
      361 gcacagccaa ccggcgacct acggtgtcgg ctcggggcga caaccgctcg tgtggcagcg
      421 tttcccagga cggcagctcg gcgaccacac cggcgcccag cacgtcggcc acctcggcgg
      481 tcagctcgcc ggcctcccgg tcagtggcgg tgacggcgag caccggatgg cctgcgcggg
      541 ccagcgcggc gacagccaac ggacgggtcg ccggcggccc cagcagctgc atctcggggg
      601 acctcgcccc ctcgaccaac gcgcgcaggg agggctccgc cagcacggcc tccagcaggc
      661 cggacaggtg agcggatgtg gtcggtagcg cagtcgctac gggcaccgtg gtgagccttt
      721 cgggcagacg tagtcaactc gaagtcgacg cagacagacc cctgcccggg acgcaaccgg
      781 cgcaccgggg tcgcaacacc cacgataccg gccggcccgc ccccggaccg ccgagggcac
      841 gttctacgtg ctcggacgcc accgcattag gtgccgagtt cggcgatctc ggtaatcaga
      901 tcgcaacgtc gcgttgtatg ctgaaggcga tcgatcgctt atccaactgc attatggcgt
      961 gtcttctggt actgcgcccg ggccagaatg agcgatttcc tagcaagtaa atgcaatggt
     1021 gaggtgggaa tagtggcgat cgatggaggg tggacgcgtt cggcccatcc ggtgggcgcg
     1081 acatgaccag catcgtgctc ggtgacgacc acgtggtgtt cgtggaatcg ctggtctccg
     1141 tgctggtcgg cgcgggattc cgggtgccgg cagtgggccg gagtctggcg gggaccatcg
     1201 aagcggtccg tcaccatcgg ccggacgtct gcctgttgag ccgtcatttc tccgatggcg
     1261 acggagtaac cgcgatcggc cctattctgg cggtaagccc ggcaactcgg atactcgtgc
     1321 tcaccgcaga gggtgatacc gatgcgatgc gccgcgccgt gcggttggga gccgccggct
     1381 atgtggacaa aacatggggc gtccgcaagc tggtccaggc gctgcagcgg gtcgtcaacg
     1441 gcgcggtggt gctggacgca ccccgggcga ccactgcgcg atccgacatc tccgaagcgc
     1501 aacggctcgc tgggcacctg acggctcgtg agagggaatg cctggcactg ctcgtcgagg
     1561 gactcgatac ccgtgcgatg accctacggc taggcgtctc tatcaccacg gtacgaagtc
     1621 acgtgcaggc actgctgacc aaactcggag tgcactcgcg gctggaggct gccaccttcg
     1681 ccgtgcgcta tgaccttgtc gatcataatc agcacggccc ggatggacgc atcaagacgg
     1741 ggtagccg
//
LOCUS       DATADN010000233         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1228419, whole genome shotgun sequence.
ACCESSION   DATADN010000233 DATADN010000000
VERSION     DATADN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228419"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VHH53_03135"
     CDS             <1..346
                     /locus_tag="VHH53_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HEX2289193.1"
                     /translation="TGFNPAVSKNALIAIGARVRSWRLHKCTQFSEVDLARWINPIVR
                     GWMSYYGAFYRSALYPLLARINAYLMRWLRKKHKRLRGRKKAHDAWGRAVAQRPGFFA
                     QWRWVTWVPPVW"
     gene            complement(441..1271)
                     /locus_tag="VHH53_03140"
     CDS             complement(441..1271)
                     /locus_tag="VHH53_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041241516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2289194.1"
                     /translation="MIAHRAMLDVPRALAQYVGRLLRAERRARRTRRDSRALTCFHQA
                     VFALRWFRDSRDITALARDHAISRATGYRYLDEVIGVLAAQAPDLHEALQQAKDNGAN
                     HVIMDGKLFASDRLGEQTTSVKGQQIDAWYSGKAHAHGGNIQALSAPDGFPLWVSEVE
                     PGSVHDMRAARTHVLAALYWAASHLELPTLADGGYDGAGIGVFTPVKQPTGGRELDVD
                     TRTYNALLRGLRCLGERGFALLTGRWRALRHITTSPRKIGKIVKAALVLTHFEHGRLT
                     "
BASE COUNT          255 a          467 c          525 g          265 t
ORIGIN      
        1 caccgggttc aatccggcgg tcagcaagaa cgccttgatt gccatcggtg cacgggtgcg
       61 gtcgtggcgg ctgcacaagt gcacacagtt ctccgaagtg gacctcgcgc gatggatcaa
      121 tccaatcgtg cggggttgga tgagctacta cggggcgttc tatcgctccg cgctctatcc
      181 cctgctggcg cgcatcaacg cctacctgat gcggtggctg cggaagaaac acaaacggtt
      241 acggggccgg aagaaggccc acgatgcatg ggggagggcc gtggcgcaac gccccgggtt
      301 cttcgcccag tggcgatggg tcacgtgggt cccaccggtc tggtgatcag aacgacaaga
      361 gccgtgtaac gggagactgt tacgcacggt tctgtgagag ccggagggtg aaactccctc
      421 cggctactcg acagcgacaa tcaagtgagc cggccatgtt caaagtgggt gagcacgagc
      481 gcggctttga cgattttgcc gatcttgcgt gggctggtgg tgatgtggcg aagtgcgcgc
      541 cagcggccgg tgagtagcgc gaagcctcgt tcgcccaggc agcgcagccc gcgcagcagg
      601 gcgttgtaag tgcgcgtgtc cacgtcgagc tcgcggccgc cggtgggctg tttgaccggc
      661 gtgaacacgc cgatcccggc accgtcgtag ccgccatcgg caagggtggg tagctccagg
      721 tgggaggcgg cccagtacag ggcggcaagc acatgggtgc gggctgcgcg catgtcgtgg
      781 accgagcccg gttcgacctc ggatacccac agcgggaagc cgtcgggcgc cgagagcgcc
      841 tggatgttgc cgccgtgggc gtgggcctta ccggagtacc acgcgtcgat ctgctgtccc
      901 ttgacgctgg tggtctgctc gcccaggcgg tcgctggcga acagcttgcc gtccatgatg
      961 acgtggttcg cgccgttgtc cttggcctgc tgcagggcct cgtgcaggtc tggcgcctgt
     1021 gcggcgagca cgccgatgac ctcgtcgaga tagcggtacc cggtggcccg ggagatggcg
     1081 tggtcacggg ccaaggccgt gatatcgcgg ctgtcgcgga accaccgcag cgcgaacact
     1141 gcctggtgaa agcaggtcaa cgccctgctg tcgcggcgag tgcgccgggc gcggcgttcg
     1201 gcgcggagca ggcgcccgac gtactgggca agtgctcgag gcacgtcgag catggcacga
     1261 tgagcaatca aggggagcct ctgcgttcgg acttgatctt cgcaaatcca gtcctaccag
     1321 gagctccccg cccacgttca ccggcccggg cgacagtcca cgcccccgat caggccgact
     1381 ggccgatgtc ctcacacgcc gaccagtcag cacgatcttg atgtcgagta gccggaggga
     1441 atttcaccct ccggctctca cagaaccgtg cgtaacagtc tcccgttaca cggctcttgt
     1501 cgttctgatc ac
//
LOCUS       DATADN010000234         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1382277, whole genome shotgun sequence.
ACCESSION   DATADN010000234 DATADN010000000
VERSION     DATADN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382277"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1070)
                     /locus_tag="VHH53_03145"
     CDS             complement(87..1070)
                     /locus_tag="VHH53_03145"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX2289195.1"
                     /translation="MRHLLTATDLQRETATALLDTAEALKQALLGREVRKLPTLRGRT
                     VVTLFYESSTRTRASFEIAGKWMSADVVNVSAAASSVAKGESLRDTALTLAAMGADCV
                     VIRHPASGAAHRLAGWVPAHVINAGDGTHQHPTQALLDAATLRERLGTLNGRTVTIVG
                     DILHSRVARSNIELLITLGARIHLVAPPTLLPCGVENWEFANLDVKKGSGQGQEPRIR
                     PVTVGHDLDAALPGTDALMLLRVQAERMHGGFFPSAREYSIRYGLSEARLGLLPEHAV
                     VLHPGPMLRGMEIAHSVADSPRAAITDQVRNGVHVRMAVLYHLLAGQEEPV"
     gene            complement(1067..>1611)
                     /gene="pyrR"
                     /locus_tag="VHH53_03150"
     CDS             complement(1067..>1611)
                     /gene="pyrR"
                     /locus_tag="VHH53_03150"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX2289196.1"
                     /translation="VLGVVQPMMSGLGGDTFLLYHDRAARQTWAINGSGPAPRALTLD
                     HLDAYHGGRLPVRGMISVGVGILDITLYRDDLRRRPTRPLQPTELPAGGLDDALVVLV
                     DDVLCSGRTVGAALDAVRDYGRPRAIQLAVLVDRGHRELPIRADYVGKNLPTSHDEEV
                     GVLLVETDGADAVVIRRGPR"
BASE COUNT          279 a          553 c          532 g          247 t
ORIGIN      
        1 ctcggcgatc atcccgtccc ggatcagcag atccaccggc gctgctgcgc cgtagggccg
       61 gatcccgcga agaaccagcg gtcgcgtcat actggttcct cttggcccgc gagcaggtga
      121 tacagcaccg ccatccgcac gtgcaccccg ttgcgaacct ggtcggtgat cgccgcgcgc
      181 ggcgagtccg cgaccgaatg cgcgatctcc atgccgcgca acatgggccc cggatgcagc
      241 accacagcgt gctcgggtaa cagccccagc cgggcctcgg aaagcccgta gcggatcgag
      301 tactcccggg cggacgggaa gaaaccgcca tgcatccgtt cggcctgcac gcgtagcagc
      361 atcagcgcgt cggtacctgg cagcgcagcg tcgagatcat gcccaaccgt taccggccgt
      421 atccgcggct cctgaccctg gccactcccc ttcttcacgt caaggttcgc gaattcccag
      481 ttctcgaccc cacaaggcag cagggtgggc ggcgcgacga gatggatccg agcacccagc
      541 gtaatcaaga gctcgatatt cgaacgagcg acccggctat gcagtatgtc gcctacgatg
      601 gtgacggtgc gcccattcag ggtgcccaaa cgctcacgca gcgtagccgc gtccagcagt
      661 gcctgcgtcg ggtgctggtg ggtaccgtca ccggcgttga tcacgtgagc gggcacccag
      721 ccggccagcc gatgcgccgc gcccgacgct ggatggcgaa tcaccacgca gtccgcgccc
      781 atggcagcga gggtgagcgc ggtgtcccgc agtgactctc ccttggccac tgaagacgca
      841 gcggccgaga cattgaccac gtcggcgctc atccacttgc ccgcgatctc gaaggaggcg
      901 cgggtgcgag tggagctttc gtagaacaag gtgaccacgg tgcgcccgcg caacgtcggc
      961 agcttgcgca cttcgcgacc cagcaatgcc tgcttgagcg cctcggcagt atccagcaat
     1021 gcggtggcgg tctcgcgctg caggtcagtg gccgtcagta ggtgcctcac cgcggccccc
     1081 ggcggatcac aacggcatca gcgccatcgg tctcgacaag cagcacaccg acctcctcgt
     1141 cgtgggaggt cggcaggttc ttgcccacgt aatcggcccg gattggaagt tctcggtggc
     1201 cccgatcgac cagcacggcg agttgtatcg cccgggggcg gccgtagtcg cgtaccgcgt
     1261 ccagtgccgc cccgacggtg cggccggagc acagcacgtc atcgaccagc acaaccagag
     1321 catcgtctag cccgcccgca ggcaactcgg tgggctgtag cggtctggta ggcctgcgcc
     1381 gcaagtcatc ccggtagagc gtgatatcca ggatgcccac gccgacggag atcatgccac
     1441 ggaccggcag gcgcccgccg tgataggcgt cgaggtggtc gagggtcagg gcgcggggtg
     1501 ccgggccgct gccgttgatc gcccaggtct ggcgggcggc gcggtcgtgg tagagcagga
     1561 aggtatcgcc gccgagaccg ctcatcatcg gctgcacgac gccgagtact c
//
LOCUS       DATADN010000235         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_780576, whole genome shotgun sequence.
ACCESSION   DATADN010000235 DATADN010000000
VERSION     DATADN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780576"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VHH53_03155"
     CDS             complement(<1..857)
                     /locus_tag="VHH53_03155"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2289197.1"
                     /translation="MSTVPTAMIFDDHDVRDDWNTSSAWCAWVTAKPWWPQRIRGALA
                     AYWIYQYIGNLPPKERHSDPTWRAVQEAGGDAWPVLQAMADAADADPSAIQWSFRWDV
                     DGVRLVMVDTRCGRILTEGRRTMLGDAEFSWVEDAVSADVHQAQHVLVGSSLPWLLGP
                     AIHDVESVNEMACARGSALGERIRQALDLEHWAAFRNSFDRLSALLQRVSAAPHAPKT
                     ISVLSGDVHHSYVAQADFSGSVGGSPVYQLVCSPVHHSIPWYAAALMRAAWFGPLVAV
                     VRSMVRRYG"
     gene            complement(871..1464)
                     /locus_tag="VHH53_03160"
     CDS             complement(871..1464)
                     /locus_tag="VHH53_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289198.1"
                     /translation="MPPSSPCARLLLGPALRHVGRTTAAVWVQTDRPAEVEVLGCRAS
                     TFTVREQHYALVEVNGLEPGSVTPYQVRLNGRLAWPAPGSPWPDSRIRTRGGSAPVRV
                     VFGSCRHAKSEDPRSAAAEGVDALDVQASRMADMAPEDWPDALLLLGDQIYADDPTPQ
                     TRRWLYSRRRDIPASPDYEVGDFTEYASAVPRQLGKR"
BASE COUNT          279 a          546 c          517 g          244 t
ORIGIN      
        1 atcccgtagc ggcgcaccat gctgcgaacc actgctacca aggggccgaa ccaggcggcc
       61 cgcatcaacg ctgcggcgta ccacgggatg ctgtgatgca ccggcgaaca caccagttgg
      121 tagacgggtg atccgcctac tgacccgctg aagtcggctt gtgccacata actgtggtgc
      181 acatcgccgg acagcacact gatcgtcttg ggtgcatggg gcgcggctga cactcgctgc
      241 agcaacgcgc tcagccggtc gaaggaattc cggaacgccg cccagtgctc gaggtcgagg
      301 gcctgacgga tgcgctcacc cagcgcgctg ccgcgagcgc aggccatctc gttgaccgac
      361 tccacgtcgt ggatcgccgg acccagcagc catggcagcg agctgccgac cagcacatgc
      421 tgggcttggt gaacgtccgc actgactgcg tcctccaccc aggagaactc ggcgtcgccg
      481 agcatcgtgc gccggccctc ggtcagaatc cgtccacacc gggtgtccac catcaccagc
      541 cgcaccccgt cgacgtccca gcgaaagctc cactggatcg ccgagggatc ggcgtcggca
      601 gcgtcggcca tcgcctgcag tactggccag gcgtccccac cggcctcctg cacagcgcgc
      661 caggtcgggt cggaatggcg ctccttggga ggcaggttgc cgatgtattg atagatccag
      721 taggcggcga gcgctccccg gatacgctgg ggccaccaag gcttggcagt gacccatgcg
      781 caccatgccg atgacgtgtt ccagtcatcc cgaacatcat ggtcgtcaaa aatcatcgcg
      841 gtgggcacgg tggacatcag ccaccggatt tcagcgcttg cccagctgtc gcggtacagc
      901 gctggcatac tcggtgaaat ctccgacctc gtaatcgggt gaggcaggga tgtcccggcg
      961 acgcgagtac aaccagcgcc gggtctgcgg agtcgggtcg tcggcgtaga tctggtcgcc
     1021 gagcagcagt agcgcatcag gccagtcctc gggagccatg tcggccatcc ggctggcctg
     1081 gacgtccaac gcgtcaacac cttcggcggc ggccgatctg gggtcctcgc tcttggcgtg
     1141 gcggcacgag ccaaaaacca ctcgcaccgg ggcactgccg ccccgggtgc ggatccggct
     1201 gtccggccac ggtgagccgg gcgcaggcca ggcaagcctc ccgttcaggc gcacctggta
     1261 gggagtgacc gaacccggtt ccaacccgtt gacttcgacc agcgcgtagt gctgctcgcg
     1321 gacggtgaac gtcgatgccc ggcagcccag tacctccacc tcggccgggc gatcagtctg
     1381 cacccacacc gctgcggtcg tcctgcccac atgccgcagc gccggcccga gcagcaaccg
     1441 ggcgcagggg gacgacggcg gcatggtcac cgttcctgcc taccgcagtc ggcgcggttg
     1501 cgcgagaata agtgcaatag tgacaaatcc ttccccggcg cgggtgagga tgggtacacc
     1561 cgccgggcgg tgggcgctgc ccacaa
//
LOCUS       DATADN010000236         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_329373, whole genome shotgun sequence.
ACCESSION   DATADN010000236 DATADN010000000
VERSION     DATADN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329373"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VHH53_03165"
     CDS             complement(<1..185)
                     /locus_tag="VHH53_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289199.1"
                     /translation="MMGKSRWIPALISFLCLALVAVACVATSGNDRRDGSGGGPGETG
                     ARGDIGDGSYRAPQIPSG"
     gene            complement(200..1906)
                     /locus_tag="VHH53_03170"
     CDS             complement(200..1906)
                     /locus_tag="VHH53_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HEX2289200.1"
                     /translation="MTALILGAALAACTGQPLPLVQPGNGPVSTPVAVPAEQEIVLGV
                     DRLAGGFNPHNLADLTPLSQAVAGLLLPSAFHQTPAGQWVLDDTLLDSAEVTDTAPFT
                     VTYRIRRNAQWSDITPIAAEDFGYLAEQMRSQPGVLDSAGYQLIDQVVSRDGGKTADV
                     VFHRPYAGWRTLFRHLLPAHLLKDAPGGWSKGFDAGLPVSGGPFAMVSVDPSRGEMVL
                     VRNDRYWDTPAQVDRILLRQGSDAALVDALRTGGAQAAFFSRPNAITESLLRAANLGV
                     TPAIVPQPVLVSVLLRPASQVLDDQRMRTAVAAALDRPSLIATGVASGPSAALRADST
                     VLAPSAPGYQATAPAAGVPMAPVPDTVRQALTAAGYNRGPVHWERAGQPLRLVVAAPA
                     DREPYGTLAARVVDQLRTAGIDAELREVDPDELFGTMLGPTPATGGESFRPASVTVDD
                     GVDIAVLPQPAAGHPATQLASWYGCPLVVPARLAPAPPNPAGLCDLTLQPFIERALVQ
                     DDPVGAVEPTVEAALWDRVVTIPLYQQVSLLVSTSQLSDPAPGTALEGPLDGAARWQL
                     HR"
BASE COUNT          342 a          747 c          735 g          305 t
ORIGIN      
        1 ccggacggaa tctgaggggc ccggtatgat ccgtctccga tgtcgccgcg cgcgcccgtc
       61 tcccctggcc cgccgccgct gccgtcgcga cgatcattcc cgctggtggc cacgcaagcg
      121 acggcgacca acgcaaggca cagaaagctg atcagggctg ggatccacct cgacttcccc
      181 atcacagaga tctcctcccc taccgatgaa gctgccagcg cgcggcaccg tcgaggggac
      241 cttccagcgc cgtccccggc gccgggtcgc tcaactggct tgtgctgacc agtagcgaaa
      301 cctgctggta aagcgggatc gtcacgaccc gatcccacag cgccgcctcg acggtcggct
      361 ctaccgcgcc caccgggtcg tcctggacga gcgcgcgctc gatgaacggc tgcaaggtca
      421 ggtcgcacaa tcctgccggg ttaggcggcg cgggggccag cctggccggt acgaccaatg
      481 ggcagccgta ccaggatgcc aactgggtgg cggggtggcc ggccgcaggc tgcggcagca
      541 cagcgatgtc gacaccgtcg tcgaccgtca ccgacgctgg gcgaaacgac tccccgcccg
      601 ttgccggcgt gggaccgagc atcgtgccga acagctcatc ggggtcgacc tcacgcagct
      661 cggcgtcgat cccggcggtc cgaagctgat cgaccacccg cgcggcgagt gtcccgtagg
      721 gttcgcgatc agccggcgcc gcgaccacca accgcaacgg ctgccctgcc cgctcccagt
      781 gcaccgggcc gcggttatac ccggcggcgg taagcgcctg gcgcacggtg tccggaaccg
      841 gcgccatagg cacaccggcg gccggcgcgg ttgcctgata gcccggcgct gatggcgcga
      901 gcaccgtgga atcagcccgc agtgccgcgg agggcccact ggcgactccg gtggcgatta
      961 gcgaggggcg gtccagtgcc gcggccaccg cggtgcgcat ccgctggtca tccagcacct
     1021 ggctggccgg gcgcagcagc accgagacga gcaccggctg tggcacgatc gccggggtca
     1081 cccccagatt tgcggcgcgc agcaatgact cggtgatggc gttggggcgg ctgaagaaag
     1141 ctgcctgcgc accaccggtg cgcagcgcat ccaccagggc cgcgtcgctg ccttggcgga
     1201 gcaggatccg atctacctgc gccggggtgt cccagtaccg atcgttgcgt accagcacca
     1261 tctcaccgcg cgacgggtct accgagacca tcgcgaacgg gccaccggag accggaagac
     1321 cggcgtcgaa gcccttgctc caaccaccgg gcgcatcctt gagcaggtgc gcaggcagca
     1381 ggtgccggaa tagagtgcgc cagccggcgt agggacggtg aaagaccacg tcggcggtct
     1441 tgccgccgtc tcgcgacacc acctggtcga tcagctggta gccggccgag tccagcacac
     1501 cgggctggct gcgcatctgc tcagcgaggt agccgaagtc ctcagcggcg atgggggtga
     1561 tgtccgacca ttgcgcgttg cgccggatcc ggtaggtgac cgtgaatggt gcggtatcgg
     1621 tgacctcggc cgaatccagc agcgtgtcgt cgagcaccca ctgccccgct ggcgtttggt
     1681 gaaacgctga gggcagcagc aggccggcga ccgcctgtga cagcggtgtc agatccgcta
     1741 ggttgtgcgg gttgaagccg ccggccagcc gatctacccc gagcactatc tcctgctcgg
     1801 caggcacggc caccggagtg ctcactggac cgttgcccgg ttggaccaac ggcagcggtt
     1861 gtccggtaca tgcggcgagc gcagcaccga ggatcagcgc cgtcaccgcc ctgcgcgcgc
     1921 caccgggcct tctcacgagg cgatatggtg ccgtagggcg atcgccgcgg ggacagggca
     1981 tcccggggct tgcgctcagg actatgtccg aggctatgac tgggactatg tccggggcta
     2041 tgtccgagcc acccgctccc gggaacgctg ccgggcgcgc tgggtggcgt ccagcaccgc
     2101 cttacggacc cgcactacgt cgggcgcca
//
LOCUS       DATADN010000237         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_478670, whole genome shotgun sequence.
ACCESSION   DATADN010000237 DATADN010000000
VERSION     DATADN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478670"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VHH53_03175"
     CDS             complement(<1..1030)
                     /locus_tag="VHH53_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2289201.1"
                     /translation="MDFEMSAKAQDYHKRLTAFMTEFIFPAEAEYHRYREEAGPKDHT
                     VPPIVEELKALARERGLWNLFLPSISGLTNLEYAPLAELSGWSLEIAPEVLNCAAPDT
                     GNMETLHLFATEEQRRQWLDPLLNGEIRSAFAMTEPAVASSDARNIETTMLRDGNDYV
                     INGRKWWITGAADPRCKILIVMGRTNQDAASHQQQSMILVPVDTPGVDIQRSLPVFGW
                     QDQHGHCEIVFDNVRVPVENLLHEEGSGFAIAQARLGPGRIHHCMRALGAAERALALM
                     VDRVQKRVAFGKPLSEQGVVREAIAKSRNEIDQARLLCEKAAWTIDQRGNKEAHVLVS
                     QIKSVAPQV"
     gene            complement(1053..1307)
                     /locus_tag="VHH53_03180"
     CDS             complement(1053..1307)
                     /locus_tag="VHH53_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289202.1"
                     /translation="MSNISENDRIEDVAPGDLVLVDRGAGPVPCKVVHKDDTDGTCVV
                     TYEVDDGTTFQVEYAAGSRVTRSLEAKWESGQSPTQHKPL"
     gene            complement(1342..1581)
                     /locus_tag="VHH53_03185"
     CDS             complement(1342..1581)
                     /locus_tag="VHH53_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289203.1"
                     /translation="MTTTTHRFARFFALPVVCAGIVAGALGFAGAASAGTYSHDMTPQ
                     PTTTAPAPVTAHPAPGAHPHHGIPHLTYVQPDYHR"
BASE COUNT          240 a          522 c          555 g          315 t
ORIGIN      
        1 ccacttgggg cgctacggat ttgatctggc tcaccagcac gtgcgcttcc ttgttgccac
       61 gctggtcgat ggtccacgcg gccttctcgc acagcagccg cgcctggtcg atttcgttgc
      121 gcgacttggc gatcgcttca cgcacaacac cctgctcgga gagcggcttg ccgaaggcga
      181 cgcgcttctg tacccggtcg accatcaacg ccagcgcccg ctcggccgcg ccaagcgccc
      241 gcatgcagtg gtggatacgg ccgggaccca gccgcgcctg ggcgatcgcg aacccgctgc
      301 cctcctcgtg gagcaggttc tcgaccggga cccgcacatt gtcgaaaacg atctcgcagt
      361 gtccatgttg gtcctgccag ccgaacaccg gaagtgagcg ctggatgtcg acaccggggg
      421 tgtctaccgg caccaggatc atcgactgct gctgatggct ggccgcgtcc tgattggtgc
      481 ggcccatcac gatcagaatc ttgcaacgcg ggtcggcggc tccggtgatc caccatttac
      541 ggccgttgat cacgtagtcg ttcccgtcac gcagcatcgt cgtctcgatg ttgcgggcgt
      601 ccgacgacgc caccgccggt tcggtcatcg cgaaggcact gcgaatctcg ccgttgagca
      661 acggatcaag ccattgcctg cgctgctcct cagtggcgaa caggtgcagc gtctccatgt
      721 tgccggtgtc cggcgcggcg cagttgagca cctcgggcgc gatctccagg ctccagcccg
      781 aaagctcggc gagcggcgcg tactccagat tggtcaggcc cgagatcgac ggcaggaaca
      841 ggttccagag cccgcgctcc ctggcgaggg ccttcaactc ctcgacgatc ggcggaacgg
      901 tgtggtcctt gggtcccgcc tcctcgcggt agcggtggta ttcggcttcg gcggggaaga
      961 tgaattccgt catgaacgcg gtcaggcgct tgtggtagtc ctgtgccttg gccgacatct
     1021 cgaagtccat gcggccacga tatgacaccc ggtcagagcg gcttgtgctg cgtcgggctt
     1081 tgcccggact cccatttcgc ctcgagcgag cgggttacgc ggcttccggc ggcgtactcc
     1141 acctgaaagg tcgtaccgtc gtcgacctcg taggtgacca cgcacgttcc gtcggtgtcg
     1201 tccttgtgga ccactttgca cgggacgggg ccggcgccgc ggtcgacgag cacgaggtcg
     1261 ccgggcgcca cgtcctcgat gcggtcgttc tcagagatgt tcgacatccc cccgacggta
     1321 gcaacgccct ccgtgtgcgt ctcagcggtg gtagtcgggc tgcacgtacg tcagatgcgg
     1381 gatgccgtgg tgcggatggg cgcccggcgc gggatgtgcg gtgacgggtg caggggcggt
     1441 cgtggtgggt tgcggcgtca tgtcgtgcga gtaggtgccc gcgctggcgg cgccggcgaa
     1501 gcccaaggcg cctgcgacga ttccagcgca gacaactggc agcgcgaaga agcgggcgaa
     1561 gcggtgagtg gtggtggtca ttgtgatcct ctgtgtttgt tgggtttcgg tgctggctcc
     1621 agcctgcccc gg
//
LOCUS       DATADN010000238         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_21847, whole genome shotgun sequence.
ACCESSION   DATADN010000238 DATADN010000000
VERSION     DATADN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21847"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VHH53_03190"
     CDS             <1..252
                     /locus_tag="VHH53_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289204.1"
                     /translation="SFAPFGRSGTEPPSNGVRTSDKAVPGRVGLRHDTGNDLKAADAS
                     QDSVAGSAGEVAQTSSSAADSEPSSTSEDSPNDASGSES"
     gene            267..647
                     /locus_tag="VHH53_03195"
     CDS             267..647
                     /locus_tag="VHH53_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289205.1"
                     /translation="MNSGRLGLVGLSAASTLLAASLAAPAHASPPSYGSNGVFGVTTN
                     PRDGWVAAFIPPGHYRVDQAPSMAPYQSAQGYWFRCHNFPCGPSFPGNVIASAPAVRG
                     VPTFMDILPTDVGVSLNNVTLTAA"
     gene            complement(644..2110)
                     /locus_tag="VHH53_03200"
     CDS             complement(644..2110)
                     /locus_tag="VHH53_03200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX2289206.1"
                     /translation="MIEHVFESVPLELDVPEMEVSDYTLLQVMGEFTRDESTAIARRL
                     MAVAELYTRRVAERADTVLAALDPIDAVAAEVSAVQNISHARAVAQVNLARALRERLP
                     EVLRLFLRGWIDFRMVSMIDRRTDNIVDEKVMARVDAALAARCEKWMKLSKTKLRDRI
                     DMVIAAHDPAGVRPPNLDEASRYVDVDAHPAAMGLAIVSGNLNAADAAALTQRLDALA
                     ATVCADDPRTQAQRRADACGPLARLEATLACQCGREDCPAATQHAAANAAVIHLLAEQ
                     ATLDGAGDTPGYLPGFGVLPAAQVRALAATAELKPVTLPAQADPGYRPSAAARDFLRW
                     RDLMCCWPGCDQPVERADVDHTVPWPYGPTHPSNNKHYCRTHHLLKTFCGWVDRQLPD
                     GTIVLTAPSGHTFTTTAHGAALFPALGQPTGWLDIGSPPPDNGADRLALMPRRTQTRD
                     QNRRDRITAERRQQSQLIAQRQRQQQAWQAKTYQPPPF"
BASE COUNT          353 a          731 c          721 g          356 t
ORIGIN      
        1 tctttcgcgc cgttcggtcg ctcgggtacg gagccaccgt caaatggcgt gcgcacaagc
       61 gataaagccg tgcccggcag ggtcgggctc cgtcacgaca cgggcaacga cctgaaagcg
      121 gctgacgcat cgcaggactc ggtcgccggg agtgccggcg aggtggcgca gacatcttcg
      181 agcgcggcgg acagcgagcc ttcctccacg agcgaggatt cgccgaacga cgcaagcggc
      241 tccgagtcct agatacactc caacctgtga acagcggacg cctcggcctc gtcgggctca
      301 gtgcggcgag cacgcttctc gcggcgtccc tggccgcgcc ggcccacgcg agcccaccta
      361 gttatggcag caacggcgtc ttcggggtca ccaccaaccc gcgcgacggt tgggtcgccg
      421 cgttcattcc acccggccat taccgagtgg accaggcgcc gagtatggcg ccttaccaga
      481 gcgcgcaggg gtactggttc cgctgccaca acttcccttg tggcccaagc tttccgggca
      541 atgtcatcgc atcggcaccc gccgtccgtg gcgtgcccac cttcatggac atcttgccca
      601 ccgacgtcgg cgtctctctg aacaacgtca cgctgacagc cgcctaaaag ggtggtggtt
      661 ggtaggtttt ggcttgccag gcttgttgtt ggcgttgtcg ttgggcgatg agttggcttt
      721 gttgtcggcg ttcggcggtg atgcggtcgc ggcggttttg gtcgcgggtt tgtgtgcgcc
      781 gcggcatcag cgctaatcga tcagcgccgt tgtcgggcgg cggtgatccg atatcgagcc
      841 agccggtggg ctggcctagc gcggggaaca gcgccgcgcc gtgggcggtg gtggtgaagg
      901 tgtggccgga gggggcggtc aacacgatgg tgccgtcggg caactgtcga tcgacccagc
      961 cgcaaaacgt tttgagcaga tggtgggtgc ggcagtagtg cttgttgttc gacggatggg
     1021 tcggcccgta gggccagggc acggtgtggt cgacatcggc gcgctcgacc ggctggtcac
     1081 agccgggcca gcagcacatc aaatcgcgcc agcgcagaaa atcgcgcgcc gccgcactgg
     1141 gccgataccc cggatcggcc tgagcgggaa gggtgactgg tttcagctcg gcggtggcgg
     1201 ccagcgcgcg cacctgcgcg gcgggcaaca caccgaatcc gggcagatat cccggcgtgt
     1261 caccggcgcc gtcgagggtg gcttgttcgg ccagcagatg gatcaccgcg gcgttggccg
     1321 ccgcgtgctg ggtcgcagca gggcagtcct cgcgcccgca ctggcaagcc agcgtggcct
     1381 ccagccgcgc cagcggcccg caggcatcgg cgcggcgctg cgcctgggta cgcggatcat
     1441 cggcgcacac cgtggcggcc aacgcatcca ggcgttgggt caacgccgcg gcgtcggcgg
     1501 cgttgaggtt gcccgacacg atcgccaaac ccatcgccgc cgggtgggca tcgacatcga
     1561 catagcggct ggcctcatcc agattcggtg ggcgcacccc ggccgggtcg tgggcggcga
     1621 tcaccatgtc gatgcgatcg cgcagcttgg tcttcgacaa cttcatccac ttctcacacc
     1681 gcgcggcgag cgcggcgtcc acgcgggcca tcaccttctc gtcgacgatg ttgtcggtgc
     1741 gccggtcgat catcgacacc atccgaaaat cgatccagcc ccgcaaaaac aaccgcagca
     1801 cctcgggcag ccgctcacgc agcgcgcgcg cgaggttgac ctgggccacc gcccgggcat
     1861 ggctgatgtt ctgcaccgcc gacacctcgg cggccaccgc atcgatcgga tcgagggcgg
     1921 ccaacaccgt atcggcgcgc tcggccaccc gacgggtata caactccgcg accgccatca
     1981 accgccgcgc gatcgccgtg gattcatcac gggtgaactc acccatcacc tgcaacaacg
     2041 tgtaatcgga cacctccatc tccgggacat ccaactccaa cggcaccgac tcgaacacat
     2101 gttcgatcat gccaccgccc accgacacga gccgtggccg ctagccgatc ttctccgcga
     2161 t
//
LOCUS       DATADN010000239         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_933554, whole genome shotgun sequence.
ACCESSION   DATADN010000239 DATADN010000000
VERSION     DATADN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933554"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VHH53_03205"
     CDS             <1..326
                     /locus_tag="VHH53_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX2289207.1"
                     /translation="IGVVDNEWIEAINRNGVVVARVIVSHRMPEGTVFMYHAQERTVG
                     VPRIEKTGKRGGIHNALTRLMIKPTHLIGGYAQQSFALNYHGPTGNQRDEVTTIRRRS
                     QEVTY"
     gene            358..>1757
                     /gene="narH"
                     /locus_tag="VHH53_03210"
     CDS             358..>1757
                     /gene="narH"
                     /locus_tag="VHH53_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX2289208.1"
                     /translation="MRLMAQLAMVMNLDKCIGCHTCSVTCKQAWTNRGGVEYAWFNNV
                     ETRPGQGYPRQYQDQQRWKGGWTLTKRGRLTLRSGSRFKRLLNIFANPDLPTVADYYE
                     PWTYDYDNLLSAPLSDTTPTARPTSLITGEDTAVTWGANWDDDLGGGPEQIGRDPLLA
                     KVSDKVTLEFQQTFMFYLPRICEHCLNPSCAASCPSGAIYKRSEDGIVLVDQDRCRGW
                     RQCVTGCPYKKIYFNHKTGKAEKCTFCYPRVEVGIPTVCAETCVGRLRYIGVVFYDAD
                     AVLAAASVRDDKDLYPAQLGVLLNPHDRRVVAEAEGAGISQEWIEAAQASPIYKLIVD
                     YQVALPLHPEYRTMPMVWYIPPLSPVVDVLSSTGHDGEDAGNLFGAIDALRIPVEYLA
                     GLFTAGDVGPVRAALQRLAAMRAHMRQINMGEPVRPEIAASVRLTRAEIEAMYRLLAI
                     AKYSDRYVIPSAAGSDA"
BASE COUNT          324 a          570 c          589 g          274 t
ORIGIN      
        1 agatcggcgt ggtggacaac gagtggatcg aggcgatcaa tcgcaatggc gtggtggtcg
       61 cccgggtgat cgtcagccac cggatgcccg agggcaccgt gttcatgtac cacgcccagg
      121 agcgcaccgt gggcgtgccc cgtatcgaga agaccggcaa gcgcggtggc atccacaacg
      181 cgctgacccg gctcatgatc aagccgacgc acctgatcgg cggctacgcc cagcagtcgt
      241 tcgcgctgaa ctaccacggc cccaccggta accagcgtga tgaggtcacc accatccgcc
      301 ggcgcagcca ggaagtgacc tactgatgac gctgggcgac gcttgcgggg cgaaccgatg
      361 agactcatgg cacagctggc catggtgatg aacctggaca agtgcatcgg ctgccacacc
      421 tgcagcgtca cctgcaagca ggcgtggacc aaccggggcg gtgtcgagta cgcgtggttc
      481 aacaacgtgg agaccagacc gggacaggga tacccacggc aataccagga ccagcagcga
      541 tggaagggcg gctggacgct gaccaagcgc ggccggctca cgttgcgctc cggctcgcgg
      601 ttcaagcggc tgctgaacat tttcgccaac ccagacctgc cgacggtggc tgactactac
      661 gagccgtgga cctacgacta cgacaacttg ctgtccgcac cgctgtcgga caccaccccg
      721 accgcccggc ccacatcgct gatcaccggt gaggacaccg cggtcacctg gggcgccaac
      781 tgggatgacg atctgggcgg cggacccgag cagatcggcc gcgatccgct gctggccaag
      841 gtgtccgaca aggtcacgtt ggagttccag cagacgttca tgttctacct gccgcggatc
      901 tgtgagcact gcctcaaccc gtcgtgcgcg gcatcctgcc cgtccggtgc gatctacaag
      961 cgcagtgagg acggcatcgt gctggtcgac caggatcgct gccgcgggtg gcggcagtgt
     1021 gtcaccggat gcccgtacaa gaagatctac ttcaaccaca agaccggcaa ggcagagaag
     1081 tgcacgttct gctacccaag ggtggaagtg ggcatcccca cggtctgcgc ggagacctgt
     1141 gtcggacggt tgcgctacat cggggtggtg ttctacgacg cggacgcggt gctggccgca
     1201 gcgtccgtgc gggacgacaa ggacctctat cctgcgcagc tgggcgtgtt gctcaacccg
     1261 cacgaccggc gggtggtggc cgaggccgag ggagccggca tctcccagga gtggatcgag
     1321 gccgcgcaag cgtccccgat ctacaagttg atcgtggact accaggtggc gctgccgctg
     1381 catccggagt accgcaccat gccgatggtc tggtacatac cgccgttgtc tccggtggtc
     1441 gatgtgctgt cgagcaccgg gcatgacggc gaggacgccg gcaacctgtt cggcgcgatc
     1501 gacgcgctgc gcatccccgt ggaatacctc gccggactgt tcaccgcggg cgacgtcgga
     1561 ccggtgcgtg cggccctgca gcggctggcc gccatgcggg cgcatatgcg tcagatcaac
     1621 atgggcgagc cggtccggcc ggaaatcgcc gcgtcggtac gcctcacccg tgccgagatc
     1681 gaggcgatgt accgcctgct ggcgatcgcc aagtacagcg atcggtacgt catccccagc
     1741 gcggccggct cggacgc
//
LOCUS       DATADN010000240         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_479180, whole genome shotgun sequence.
ACCESSION   DATADN010000240 DATADN010000000
VERSION     DATADN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479180"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..530
                     /locus_tag="VHH53_03215"
                     /pseudo
     CDS             167..530
                     /locus_tag="VHH53_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031169.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
     gene            527..892
                     /gene="crcB"
                     /locus_tag="VHH53_03220"
     CDS             527..892
                     /gene="crcB"
                     /locus_tag="VHH53_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX2289209.1"
                     /translation="MTAVVLVALGAALGAPARYLTDRLIQARHDSLFPWGTLAVNVAG
                     SFLLGLLAGLPVSEPVMAAIGIGFCGALTTYSTYGYETVRLAQNRARFYAVVNATASV
                     LAGLGAAYCGLAVAQALTT"
     gene            984..1496
                     /locus_tag="VHH53_03225"
     CDS             984..1496
                     /locus_tag="VHH53_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX2289210.1"
                     /translation="MWWEVLGGVVAGLVLLWLVLLVVLWRTKPEQTGLRDALRLLPDL
                     IRLLRRLAADPALPRGVRIRLALLLGYLALPIDLIPNFIPVLGYADDAIVVALALRSV
                     VRRAGPDALAQHCPAPPRTCASCTDSRSSRPHPDQRTRGDDPGAVSPLSGLPCSRSVV
                     RCCGPTSSGR"
BASE COUNT          215 a          547 c          534 g          277 t
ORIGIN      
        1 ttgaagtcca gcaggcggtg gcggccggtg ccgctcgcct ggcccttgcc tacctgggcg
       61 gaaccctggc cgcagcggtg gtcgcggtgg tggtgggctc ggccctgacg tccaccgtga
      121 tccgggtgct cacccgcgcc ggccggcacg aaccgaagga gcggtgatga aactgcatgg
      181 ttccacgctg cgcctgacga tcttcatcgg cgacaccgat ctatggcacc accgcccgct
      241 gtaccacgag atcgtgcacc gggcccacga agcgggcttg gccggcgcga gcgtgatccg
      301 cggaatggag ggctacggcg cgtcctcgcg catccacaca acgcggatct tgtcgctgtc
      361 cgaggatctg ccgctcatgg tgatcattgt ggacgccgag gaaaatccgc gcgttcttgc
      421 cgcagctcga cgagctgatc ggcgagggtc tagtgatcct tgatgaggtc gaggtgattc
      481 gctacatcgg gcgccccagc gaccggcccc gcaaggaacg ccgccggtga ccgccgtcgt
      541 gctagtggcg ctcggcgccg ccctcggtgc accagcccgc tatctgaccg accggcttat
      601 ccaggcccgc catgactcac tgttcccgtg gggcaccctc gcggtcaacg tcgccggatc
      661 gttcctgctc ggcctgctcg ccggcctgcc ggtcagcgaa ccggtgatgg ccgcgatcgg
      721 gatcggattc tgcggtgcgc tgaccaccta ctccacctac ggctacgaaa ccgtgcgcct
      781 ggcccagaat cgcgcccgct tctatgccgt cgtcaacgcc actgccagcg tcctggccgg
      841 cctgggggct gcctactgcg gactggccgt ggcccaggca ctcaccacat gagcgccccg
      901 gcaagcctgg ctagcttgct ggggcccgtt acccccggtg gcccgacacc cccgcccagt
      961 gaagtgatgg gagcgaacta aacgtgtggt gggaggtctt gggcggggtt gtggccgggc
     1021 tcgtgctgct ctggctggtg ctgctggtgg tgctgtggcg aaccaagcca gaacagactg
     1081 ggctgcgcga tgccttacgc ctactaccgg acctgatccg gctgctgcgt cgcctcgccg
     1141 cagacccggc gctgccgcgc ggagtccgga tccggctggc gctgttgctg ggctatctgg
     1201 cgctgccaat cgacctcatc ccgaacttca tcccggtcct gggctatgcc gacgacgcga
     1261 tcgtcgtcgc gctcgcgctg cgctccgtgg tccgccgcgc cgggcccgac gcgctggccc
     1321 agcattgccc ggcacccccg agaacttgcg catcctgcac cgactcgcgc agctcccgcc
     1381 cccatccgga ccagcggact cgcggtgacg atcctggagc ggtgtcgccg ctcagtggcc
     1441 tgccctgctc gcggtcggta gtccggtgtt gcggcccgac gagtagcggt cgctagtgaa
     1501 ctgtgcggat taattgaccg tggccggtcg gtagtagggg tgacggtggt ggtcaacggg
     1561 tgggcgccga gct
//
LOCUS       DATADN010000241         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_479278, whole genome shotgun sequence.
ACCESSION   DATADN010000241 DATADN010000000
VERSION     DATADN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479278"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..>1539
                     /locus_tag="VHH53_03230"
     CDS             481..>1539
                     /locus_tag="VHH53_03230"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX2289211.1"
                     /translation="MAWVEDPRARRGVRHGFVAVVGVGVCAVLAGARTFTAIAEWAHD
                     LTPAVRVRLGLGRKPPSESTIRRVLQAVDAEALDQAVSAWLVTRSAPSATALRMIAID
                     GKSAHGTRGADGRAVHLLAAFDQASGIVLGQTVVDGKTNEINAFVPLLDRIDITGAII
                     TADALHTQHRHADYLIGRGAHYVLTVKRNQPSLHRQLATLPWAQVPAVDLTHDKGHGR
                     IESRSLKLAAVPAGIAFPHAYLAIQLTRRRRPLSSGKWHTETVYAITDMTWAQIRADH
                     LAEAIRGHWGIENRLHWIRDVVFAEDHSQIRTGTGPAVMATLRNLAVSLHRLTGATNI
                     AAACRHVSRHPNPRPATTD"
BASE COUNT          254 a          556 c          472 g          257 t
ORIGIN      
        1 gttcgggaag cctctcgtgt tggacgccac tggatcatca ctacacccaa ccgctggttc
       61 cccgtggagt ctcacacgaa tgccgttttc aggcactgga ggccaagttg gcgggccagt
      121 aagccctggg cgttcacgcg cctgctgtcc aggagcgagt tcgccgacct actgccacaa
      181 ggcagccgaa tcatcggacg accgtggagc gccacattca tagcaatggg gctgggggcg
      241 cacaccaggg ccccggcaaa gtcgtctact gcggtgtgtc tgcggcttga cacgccagtg
      301 atgaggtaag agcggtgcga acacggcacc gctagtgatc atgatggttc ctacgccagc
      361 acgatcaccg gagcaactgc cgtgtccgcc tgtccatctt tgccgatctc cattggtacg
      421 acttgccccg ggcccgcgtt ggccggtggt gattctgcgc cagttgatct gcttgccgcg
      481 ttggcctggg tcgaggatcc ccgggcgcgc cgcggggtgc ggcacggatt tgtcgcggtt
      541 gtgggggtcg gggtctgcgc tgtgctcgcc ggggcccgta cgttcaccgc gatcgcggaa
      601 tgggcgcacg atctgacccc tgcggtgcgc gtccggcttg gccttggccg caagccaccg
      661 agcgagtcga cgatccgccg cgttctgcag gcggtcgacg cggaagctct cgatcaggcc
      721 gtctcggcct ggctggtgac ccgatccgcc ccctcggcca cggcgttgag gatgatcgct
      781 atcgacggca agagcgctca cgggacccgg ggagccgacg gtcgcgcggt ccatctgctc
      841 gcggcgttcg accaggccag cggcattgtc ttggggcaga ccgtggtgga cggcaagact
      901 aatgagatca acgccttcgt gccgttgctg gatcgcatcg acatcaccgg tgcgatcatc
      961 accgccgatg cgctgcacac ccagcaccgc cacgccgact acctcattgg ccggggcgcg
     1021 cactacgtgc tcaccgtcaa gcgcaaccaa cccagcctgc accgccagct cgcgaccctg
     1081 ccctgggccc aagtccctgc cgtggacctc acccacgaca aaggccacgg ccggatcgaa
     1141 tcccgcagcc tgaaactcgc cgccgtgccc gctggcatcg cctttccgca cgcctacctc
     1201 gctattcagc tcacccgccg acgccgaccg ctgagcagcg gcaaatggca caccgagacc
     1261 gtctacgcga tcaccgacat gacctgggcc cagatccgcg ccgatcacct cgccgaggcg
     1321 atccgtgggc attggggcat cgagaaccgg ctgcactgga ttcgcgacgt cgtcttcgcc
     1381 gaggatcact cccagatccg caccgggacc ggtcccgccg tgatggccac tctgcgcaac
     1441 ctcgccgtca gcctgcaccg acttaccggc gccaccaaca tcgccgccgc ctgccgccac
     1501 gtcagccgcc accccaaccc gcgtcctgcc acaactgac
//
LOCUS       DATADN010000242         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_330503, whole genome shotgun sequence.
ACCESSION   DATADN010000242 DATADN010000000
VERSION     DATADN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330503"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VHH53_03235"
     CDS             complement(<1..229)
                     /locus_tag="VHH53_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289212.1"
                     /translation="MLVYLGVGSVAAATLLYEVALTRIFAIAYGYHFAFLAMSLGLLG
                     FGASGTLLGLRPPSRQPLHSGLLARLSAAASL"
     gene            340..813
                     /locus_tag="VHH53_03240"
     CDS             340..813
                     /locus_tag="VHH53_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289213.1"
                     /translation="MLRTAALRGVAAGLAGVAVMTAAEKAEQRLTGRPSSYVPGRTLL
                     ALVAGRPPESAQPTVWNHSMHWGTGALLGGLRGVWAANGLRGPRANLVHTVVRLATDQ
                     TFENAVGVGAPPWTWPRQEQTIDVAHKAIYSFVTGWIADRVITSTPVFKAGTVSH"
     gene            943..1461
                     /locus_tag="VHH53_03245"
     CDS             943..1461
                     /locus_tag="VHH53_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX2289214.1"
                     /translation="MVTKLEKTTQADADEYRNGEERPLGGYVAAMMVFALLVATAASV
                     AALAGRSLPRRIGAYDLLLITAGTHKLSRTLSKTAVTSPLRAPFTRYQDTAGPGELTE
                     EVRQSSGLRHAIGELLTCPFCLDMWVVTAHMIGLIFVPRFTRMVAATFSALAGADFLH
                     LAYSRAMQATEN"
     gene            1509..2111
                     /locus_tag="VHH53_03250"
     CDS             1509..2111
                     /locus_tag="VHH53_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289215.1"
                     /translation="MPVRWRFDGEIAAFGTASGHRIVVGRWPVSPFGPVSDVMVEAPH
                     GTRLLIAPSDEVAGFVASTYRFDCVEVKPVEVIRQPGRLEVVAGQLRATMTVGRRSWL
                     GMLLRAVPRPVATAPAWANLVDPLVRAMLPGVRTRGSAGAGRKEWYGAWDHHRLGSVA
                     ASWSGADLGAMTDVFPPVRFGFGSAPRQPCVVRLTTTVQL"
     gene            complement(2121..>2536)
                     /locus_tag="VHH53_03255"
     CDS             complement(2121..>2536)
                     /locus_tag="VHH53_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2289216.1"
                     /translation="YQNRDDGFPGLQRTYGFQVPSDRLQVLQTGAIYQATADQQECMF
                     GEVFTTDGRIPQLGLTVLQDDKLYHPLYNAAPIMRKQAYDRNPAIAKVLAPISAALVN
                     DVVAELNRQRSAEGRSARRVARDWLAQQGFIARKS"
BASE COUNT          428 a          797 c          854 g          457 t
ORIGIN      
        1 ccagcgaggc agctgccgac aggcgagcaa gcagcccgga atgcaacggc tggcgcgacg
       61 gtggacgaag cccgagtagg gtaccgctcg ctccgaaccc cagtagcccg aggctcattg
      121 ccaggaaagc gaaatggtag ccgtaggcga tggcgaagat ccgggtgaga gcgacttcgt
      181 acaggagggt cgcggccgct accgatccca ctcccagata gacgagcacg cgttatctct
      241 cccgaaagca ccgctgccgt ctggttcgag cttcccgtgc tgtgccaccg tacctggctc
      301 gtgggtgtgt aatcggtaga tcccgggtac tcagcggtca tgttgcgaac tgctgccctg
      361 cggggcgtgg ccgctggact ggctggtgtg gcggtgatga ccgcggccga gaaggccgag
      421 cagcggctga ccgggcgacc cagctcttac gtgccgggtc ggacgttgct agccctcgtc
      481 gccgggcgcc cgcccgagag tgcccagccg acggtgtgga accattccat gcactggggc
      541 actggtgcgc tgcttggagg gcttcgggga gtctgggcgg ccaatggcct gcgcgggccg
      601 cgcgcaaacc tcgtccacac cgtggttcgg ctcgccactg accagacatt cgagaacgcg
      661 gtgggcgtgg gggcgccgcc ctggacatgg ccccggcaag agcagacgat cgacgttgcg
      721 cacaaggcca tctactcgtt cgtcaccggt tggatcgctg atcgggtcat cacctccact
      781 ccggtcttta aagcagggac ggtcagtcac tgatctggac ccggcccggt tgacgcattt
      841 tgagctttat gtgaatcaga tggctatacc cccgacgcgt aaaggatgtc tggcgcggca
      901 accgcgcgct cgactgcaac gaaataaagg agactgcaga gcatggtaac aaagctggag
      961 aagactaccc aggcagatgc cgacgagtac cgcaacggtg aggagcgtcc cctgggcggc
     1021 tacgtagcgg cgatgatggt gttcgccctg ctggtggcaa ccgcggcatc ggtggccgcg
     1081 ctggccggac ggagcctgcc ccgccggatc ggcgcctacg acctactgct catcaccgcg
     1141 ggtacccaca aactctcgcg cacgctgagc aaaactgcgg tcaccagtcc gttgcgcgcc
     1201 cccttcactc gttaccagga cacggcgggg cccggtgagc taacggagga ggtgcggcaa
     1261 tccagtggct tacggcatgc gatcggtgag ctactcacct gcccgttctg cttggacatg
     1321 tgggtggtaa ccgcccacat gatcggattg atattcgtgc cccgattcac ccggatggtg
     1381 gcagccacat tcagcgcact tgccggtgct gactttttgc acctcgccta ctctcgcgcg
     1441 atgcaagcta ctgagaactg acatcaggaa cggcgccttg atcagtgaaa tgcgagtgac
     1501 agtcggccat gcccgtacgt tggcgctttg acggtgagat cgccgccttc ggcacggcat
     1561 cgggccaccg catcgtggta ggacgatggc cggtgtcgcc cttcgggccg gtcagcgacg
     1621 tcatggtcga ggcgccccac ggcaccaggc tgctgattgc cccgagtgac gaggtcgccg
     1681 gcttcgtcgc cagcacttat cgcttcgatt gcgtcgaggt caaaccggtt gaggtgatcc
     1741 gtcaaccggg gcggctggaa gtcgtagccg gccagctacg agccaccatg acggtcgggc
     1801 gcaggagctg gctcgggatg ctgctgcgag ctgtgccgcg tcccgtggcc accgcccccg
     1861 cttgggcgaa tctcgtggac ccgctggtgc gggccatgct ccccggtgtg cgcacccgag
     1921 gtagcgccgg tgccgggcga aaggagtggt acggggcctg ggaccaccat cgactgggtt
     1981 ctgtggccgc cagctggtcg ggggcagatc tcggtgccat gaccgatgtc tttccgcccg
     2041 tccgattcgg gttcggctcg gcgccacgcc agccctgcgt tgttcgcctg accacgaccg
     2101 tgcagctatg acagcagtgg tcacgatttc ctggcgatga agccttgctg agcaagccag
     2161 tcgcgggcga cccggcgggc tgacctgcct tccgcggatc gctgccggtt cagctcggcc
     2221 accacatcgt tgaccaacgc tgcggagatc ggtgcgagca ccttggcgat ggccgggttg
     2281 cggtcgtagg cctgtttgcg catgatgggc gccgcgttgt agagcgggtg gtacagcttg
     2341 tcgtcctgca acaccgtcag cccgagctgt gggatgcggc cgtcggtggt gaacacctca
     2401 ccgaacatgc actcctgctg atccgcggtg gcctggtaga tggcaccggt ctggagcacc
     2461 tgcaaccgat ccgacgggac ctgaaaaccg taggttcgct gcagacccgg gaagccgtcg
     2521 tcccggttct ggtatt
//
LOCUS       DATADN010000243         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1084222, whole genome shotgun sequence.
ACCESSION   DATADN010000243 DATADN010000000
VERSION     DATADN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084222"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1154
                     /locus_tag="VHH53_03260"
     CDS             201..1154
                     /locus_tag="VHH53_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289217.1"
                     /translation="MTATLARSDPGGPIPAPAPGMPLPRSVPSLDEKAFDDAVKSGDW
                     ATATTSLAKLAMPAAKLAPLTIVQLRLLQDAVTRARCVLGGVGIVLQLAIAVELQGKG
                     VPAAKVTAGTAFGKLETTVGERIDGDQAAGTWYSYKIDITFTPDLTVVDAAEIAFIQT
                     VRLVETVSGSNKDPEQTSKKRQTPLATSVDRRSGKKQGWYGLKDDETGSSMLTVWKKS
                     APATPATMQDRASWNQPNTTWQFETMVACRSGTDIGKVYVAVTWGFSVDADLKLTEQA
                     PMVTNKQSAEATTAVSKWNDQAAGSASDRNTPGQVPLPTLT"
BASE COUNT          285 a          517 c          529 g          235 t
ORIGIN      
        1 ctctttcgtg tcgagcacct cgccaggagc gagcctgccc ggtcccggcg tacccgtctg
       61 cgcttcttgc tcgcccgaaa ggctgctgca cagaagatcg agaagtttgg catgatcagg
      121 acgtgcacgc gcagcctcgg atctccaacg ccagcgtgtg caagcacagg ctggcacgac
      181 cgcccgttgg cagcggcgct gtgacggcga cgctggcccg cagcgacccc ggcggcccga
      241 tacctgcgcc agcgcccggg atgccattac cgcggtctgt acccagcctc gacgagaagg
      301 cattcgacga cgccgtcaag tcgggtgact gggccacggc gacgacgtcg ctggcgaagc
      361 tggccatgcc ggcggcgaag ctggcgccgc tcacgatcgt gcagctgcgc ctgctgcagg
      421 atgccgtgac ccgcgctcgc tgcgtgctcg gcggcgtggg aatcgtgctg cagctcgcga
      481 tcgccgttga gctgcagggc aagggcgtcc cggcggccaa ggtcacggct ggcaccgcat
      541 tcggcaaact cgagaccacg gtcggcgagc ggatcgacgg tgaccaggct gcgggcacct
      601 ggtactccta caagatcgac attacgttca ctcccgacct caccgtcgtc gacgccgccg
      661 agatcgcatt catccagacc gtccgcctcg tcgagaccgt gagtggctcc aacaaggatc
      721 ccgagcagac gagcaagaag cgccagacac cgttggccac gagcgtggac cggcgcagtg
      781 gcaagaagca aggctggtac gggctcaaag acgatgagac gggcagcagc atgctgaccg
      841 tctggaagaa gtccgccccg gccacgccgg ccacgatgca ggatcgcgcg agttggaatc
      901 agcccaacac cacgtggcag ttcgagacca tggtggcctg ccgctccggg acggacatcg
      961 gcaaggtgta cgtggccgtc acgtggggct tctccgtcga cgctgacctc aagctcaccg
     1021 aacaggcacc catggtgacg aacaagcaga gcgcggaggc caccacagcg gtgagcaagt
     1081 ggaacgacca ggctgccggc tctgcctctg accgcaacac tccgggccag gtaccactgc
     1141 cgacactgac atgagcgccg gcgtccacgc agatcgacgc gcgcttaagg ccgtggattg
     1201 gtgggtatcc gataatgtga tcgccaggct ctgccgggct gcgcccggcg tgccggtgag
     1261 atgctagagg aggttgaaat gatcaaaaag attttgcagt ttctggtact gaagcggctt
     1321 tgggaccgcc gccgcggtcg ccgctgatga tgtgcgacga cgccggtctg cggggatgcc
     1381 gctgagggaa ctacgagtcg tcaccgccac cttccgcctc ggaccatcgg cggcagcggc
     1441 acgcggagtc cctccggagc cggttggcgc atcgcgtcct cgatgagcgg gtcgggttcg
     1501 cgcagtccga gttcggccgc cacgaaccgg gcggccagct ccgaccagtc gtgcgcgcgg
     1561 cggagc
//
LOCUS       DATADN010000244         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1230078, whole genome shotgun sequence.
ACCESSION   DATADN010000244 DATADN010000000
VERSION     DATADN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230078"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VHH53_03265"
     CDS             <1..876
                     /locus_tag="VHH53_03265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289218.1"
                     /translation="SADVVHLHSAKAGLAGRLALQGRRPTLFQPHGWSWLAVRGAAAR
                     AALAWERFASRWTDVVVCVGDGEAAHARHRQMLGRHVVVRNGVDLARFTPTDERARAA
                     ARDRLGVSRAAPLAVCIGRVTRQKGQDMLLRAWPQVQQHCPQAQLSIVGDGSLLRTLQ
                     GWASPSVRFVAPVTDPRPWYAAADVVILPSRWEGLSLTLLEALASGCPVVVSDIPGLA
                     DAMTASVGARVGVGNAEGLANAVARRLSDRDLRAHEATAARLAATVFDVQRTYEQLAA
                     VTIQIARPAVQYGSE"
     gene            993..2417
                     /locus_tag="VHH53_03270"
     CDS             993..2417
                     /locus_tag="VHH53_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX2289219.1"
                     /translation="MRLSAQGRLRGVDVAEHRLALAINGRERLLETRLVPFSLADGPP
                     SAGSGRARLGSVAIVGLGYVGLPTALALLGRASRITGFDVSDDRLRAIESGDVDLSDL
                     DRVRLAAAHSEDSFLLTSDSAALAESEAVIVCVPTPVDAHRIPDLTALQRACQTVVEH
                     AHSGQTIILTSTSYAGTTRQLLIEPLQQRGMTVGKDVYVAFSPERIDPGNPDHVQRET
                     PRVVGGATTECSGRAARVIGQLTDAVYLVSSPEAAELTKLYENIFRAVTLALANEFAD
                     VCGTLGLDPIEVTLAAATKPYGFLGTFPGPGVGGHCIPCDPHYLLWQLRGRDRPTPLI
                     EQAMQSIHARPARVVERAGELLTAEGRSLAGVKVIVVGASYKAGVSDVRESPALPIID
                     GLAERGADVHYHDSLVPVLRTPHGRLMRSVTAPRGADWDLAVIHVVHPRTDDNCAGND
                     WIASCPRVLDATYQFNGAPHGSVV"
BASE COUNT          382 a          882 c          908 g          399 t
ORIGIN      
        1 agcgccgacg tggtgcacct gcactcggcg aaggcaggct tggctggacg gcttgccctg
       61 cagggccggc gtcccacgct gttccagccg cacggctggt catggctggc ggttcgtggc
      121 gcggcagcgc gcgcggcgct ggcctgggaa cgcttcgcgt cacgctggac cgacgtggtc
      181 gtgtgcgtcg gcgacggcga ggccgcccac gcgcggcacc ggcagatgct gggccgccac
      241 gtcgtggtcc gcaacggcgt cgacctcgcc aggttcacac cgaccgatga gcgggctcgc
      301 gccgccgcac gggatcggct gggggtgagc cgcgcggcgc cgctggcggt gtgcatcggc
      361 cgggtcaccc ggcagaaggg tcaggacatg ctgctgcggg cgtggccgca ggtgcagcag
      421 cattgcccgc aagcgcagct gagcatcgtg ggtgacggca gcctgctgcg cacattgcag
      481 ggttgggctt cgccgagcgt gcggttcgtc gcaccggtca ccgatcctcg gccttggtat
      541 gcagcggccg acgtggtgat cttgccatcg cgctgggagg gcttgtcgct gaccttgctc
      601 gaggccctgg ccagcggttg ccccgtggtg gtatccgaca tccccggcct cgcggatgcc
      661 atgaccgcca gcgtgggcgc ccgggtggga gtgggcaacg ctgaagggct ggccaacgcc
      721 gtcgcgcggc ggctcagtga tcgtgacctg cgcgcccatg aggcgaccgc cgcccggctg
      781 gccgcgacgg tctttgacgt acagcgcact tacgaacaac tcgccgccgt cacaatacaa
      841 attgcccggc ccgcagtgca atacggctcc gaatagcggt ctccttaccg gcccacagcg
      901 caattcggct ccgaatagcg gtctgactgg gccggtcagc cgcatggcgt aggcggacta
      961 ctgctgatgg cggaggactc accgtccagc tgatgcgact gtctgcacag ggtcgactgc
     1021 ggggggtgga cgtggctgag caccggcttg cactcgcgat caacggacga gagcgattgc
     1081 tggagacacg gctggtgccg ttctcgctgg cggatggacc gccgagtgca gggagcgggc
     1141 gggcccgatt gggatcggtc gcgatcgtgg gcctgggcta tgtcgggctg ccgacggcgt
     1201 tggctctact gggaagggcg tcgcggatca ccggtttcga cgtgagcgac gaccggctgc
     1261 gcgccatcga atccggcgac gtcgacctgt ccgatctcga ccgggtacgg ctggcagcag
     1321 cgcacagcga ggacagcttc ctactcacca gcgacagcgc cgcactggcc gaatccgaag
     1381 cggtgatcgt ctgcgtgccc actccggtgg acgcccaccg catccccgat ctcaccgccc
     1441 tgcagcgcgc ctgccagacc gtcgtcgagc acgcccactc cggacagacg atcatcttga
     1501 cgtcgaccag ctacgccggc acgacgcggc agctgctcat cgagcccctc cagcagcgtg
     1561 gcatgaccgt gggcaaggac gtctacgtcg ccttcagccc ggagcgcatc gaccccggca
     1621 accccgacca cgtgcaacgc gagacgcccc gagttgtcgg cggggctacg accgagtgca
     1681 gcggccgggc cgcgcgggtg atcggccagc tgaccgacgc ggtgtatctg gtctcctccc
     1741 cggaggccgc cgagctgacc aagctttacg agaacatctt ccgcgcggtc accctggccc
     1801 tggccaacga gttcgccgac gtgtgcggca cgctcgggct ggatccgatc gaggtcaccc
     1861 tggcggccgc gaccaagccc tacggcttcc tgggcacatt ccccggcccg ggggtgggcg
     1921 ggcactgcat cccgtgcgat ccgcactacc tgctgtggca gctgcgtggt cgcgaccggc
     1981 cgacgccgct gatcgagcag gccatgcagt cgatccacgc gcgtcccgcc cgagtcgtcg
     2041 aacgcgccgg ggagctgctc accgccgagg gacgctcgct ggccggcgtc aaggtgatcg
     2101 tggtcggcgc cagctacaag gccggggtat ccgacgtccg ggaatcaccg gcgctgccga
     2161 tcatcgacgg gctcgccgag cgcggcgccg acgtgcacta tcacgactcg ctggtgccgg
     2221 tgctccggac accgcacggg cggctgatgc gcagtgtcac agctccccgc ggcgccgact
     2281 gggacctggc agtcatccat gtggtgcatc ccaggaccga cgacaactgc gccggcaacg
     2341 actggatcgc cagctgcccg cgggtcctcg acgcgacgta ccagttcaac ggtgctccgc
     2401 acggcagcgt ggtgtgaggg gtttgggatg aggcctcgcc catgccccga gctggatacc
     2461 ttgcttgata cggggacacc ggcagtgata ttcaagctcg accgcaacgt catgcaccac
     2521 ggcggcctgg gcgccatccg tagtttgggt cggctgggcg tgccggtcta c
//
LOCUS       DATADN010000245         4317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_934053, whole genome shotgun sequence.
ACCESSION   DATADN010000245 DATADN010000000
VERSION     DATADN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934053"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..867)
                     /gene="map"
                     /locus_tag="VHH53_03275"
     CDS             complement(10..867)
                     /gene="map"
                     /locus_tag="VHH53_03275"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2289220.1"
                     /translation="MPVRTPLCPGRQTPMRSVPAQIVRPEYVGKAAPTPSTDPWTQPT
                     EVIEAMRVAGKVAAQALAEGGRAVAPGFTTDDVDRVVHEFLLDHGAYPSTLGYKGFPK
                     SCCTSLNEVICHGIPDSTVIEDGDIVNIDVTAFIGGVHGDTNATFLAGDVSEEDRLLV
                     ERTHEAMMRGIRAVRPGRQLNVVGRVIEAYANRFGYGVVRDFTGHGIGRSFHGGLVVL
                     HYDEPAVDTVLEPGMTFTIQPMITLGVIDYDLWPDGWTVTTKDKQRTAQFEHTLVVTA
                     DGAEILTLP"
     gene            complement(896..2671)
                     /locus_tag="VHH53_03280"
     CDS             complement(896..2671)
                     /locus_tag="VHH53_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2289221.1"
                     /translation="MALTAVLTAGCGVFGSDPDDAAAAFLAAVSRGDDAAAGQLTDDP
                     VAATALIGQVREELKPQHVQLTLGQTRSGDNTASASFSAVWDLGQNRKWSYAGSFDLI
                     HADTAEGWLVRWSPAALHPRLAAQQQVLLREVPAEPVSVVDRSGTPLLTTQTVVTVAL
                     DRSKTSDLPGVATRLAAALARIDPEITEQSIIAAVNAAPPGQRGAVVVLREQDYESVR
                     DQIYELPGVTFPTQQRLLGPDQDFASQLVPGIRTAVEEQLAAAAGWRIVTVTTLGQEV
                     ETLAAQQPRPVPTVTTTVDRGVQGAGEGAVDAESNPAMIVAIQPSTGEILAVAQNDAA
                     DAQGALALTGRYPPGSTFKIVTASAAMQSQAVTPESPVACPAATTIGDRRVPNDQLFD
                     LGTVPLRTAFARSCNTTFAQLGARMGAGALTDAAHQMGIGIDYDVPGITTVTGQAPPS
                     ESTVRRAENGFGQGRVLASPFGMALVASTVAASGQLPTPVLIRGMPARTVIEPAGGVP
                     REVADALRAMMREAVTRGTANALAGQGQLYGKTGTAEYSLTGAHGWFVGFRGDLAFAT
                     LVVDAGSSGPALDVSERFLSTLGAA"
     gene            complement(2739..3584)
                     /locus_tag="VHH53_03285"
     CDS             complement(2739..3584)
                     /locus_tag="VHH53_03285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2289222.1"
                     /translation="MLKLASARLLDNHDARGVRAVLAINPVMSCMVAARIESGGLDPW
                     RLGGELWGFSQGPDGPLDGLCFSGPNLVPLRGGSSAIRAFADRAAAQPRSCSSMVGPA
                     DQVLPLWSRLRETWGAAREERPDQPLMVLDKQPLVPGDPLVRLVRTDEIDRYLPAAVA
                     MFTEEVGVDPRLPDGGAGYRARVAELIAAGRAFARFEGDEVVFKAEIGVMSSTVGQLQ
                     GVWVHPSRRGQNLAAGATAAVVTRLQRMGRIASLYVNAYNQPARATYRKIGFTQVGCF
                     ATVLF"
     gene            complement(3635..>4317)
                     /locus_tag="VHH53_03290"
     CDS             complement(3635..>4317)
                     /locus_tag="VHH53_03290"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2289223.1"
                     /translation="IGVNAGSLDPKILAKHGKATPEALVESALWEASLFAEHDFHDIK
                     ISVKHHDPVVMVRAYEQLAAQCDYPLHLGVTEAGPAFQGTIKSATAFGALLSQGIGDT
                     IRVSLSAPPVEEVKVGLQILESLNLRTRRLEIVSCPSCGRAQVDVYKLADEVTAGLEG
                     MEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGEVIKTVPESQIVETLIEE
                     AQRLAEEP"
BASE COUNT          727 a         1520 c         1386 g          684 t
ORIGIN      
        1 ggcggaagat cacggcagag tcaggatctc cgcgccgtcc gcggtgacca ccagggtgtg
       61 ctcgaactgt gcggtgcgct gcttgtcctt ggtggtcacc gtccacccgt cgggccacag
      121 gtcgtagtcg atcacgccca gagtgatcat cggttggatg gtgaaggtca ttcccggctc
      181 caagacggtg tccacggcag gctcgtcgta atgcagcacg accagacccc cgtggaatga
      241 gcgaccgatg ccgtgaccgg tgaagtcgcg gaccactcca tacccgaagc ggttggcgta
      301 agcctcgatc accctgccga ccacgttgag ctgccgaccc ggccgcaccg cacggattcc
      361 gcgcatcatc gcctcgtggg tgcgctcaac gagcaaccgg tcctcctcgg aaacatcacc
      421 ggcgaggaac gtcgcgttgg tatccccgtg cacgccgccg atgaaggcgg tgacgtcgat
      481 gttgacgatg tcaccgtcct cgatcacggt cgagtccgga atgccgtggc agatcacctc
      541 attcagcgag gtgcagcacg acttggggaa gcccttgtac cccagtgtcg agggataagc
      601 gccgtggtcg agcaggaatt cgtgcaccac ccgatcgacg tcgtcggtgg taaagccggg
      661 cgcgacggcc cggccgccct ccgcgagcgc ctgcgcggca acctttcccg ccacccgcat
      721 cgcctcgatc acctcggtcg gctgcgtcca cgggtcggtg ctgggggtgg gtgcggcctt
      781 gccgacgtat tccggacgca cgatctgcgc gggaacggaa cgcatcggcg tctgccggcc
      841 ggggcacagc ggggttctca cgggcatacc cacactctag gcaaccccga ccgcgttacg
      901 cggcacccag ggttgacagg aaccgctcgg agacgtccag ggccgggcct gaggagccag
      961 catcgacgac cagcgtcgcg aatgcgaggt ctccgcggaa gcccacgaac cagccgtgcg
     1021 cgccggtgag gctgtactcc gcggtcccgg tcttgccgta cagctgcccc tggcccgcca
     1081 aggcatttgc ggtgcctcga gtgaccgcct cgcgcatcat tgcccgcagc gcgtcggcga
     1141 cctcgcgcgg caccccgccc gcgggttcga tcaccgtccg agccggcatt cccctgatca
     1201 gcaccggtgt aggcagctgc ccgctggccg ctacggtcga ggccaccaac gccatgccga
     1261 acgggctggc aagcaccctg ccctgaccga acccgttctc cgcgcgccgc accgtcgact
     1321 ccgagggggg agcctgtccg gtgacggtcg tgatgccggg aacgtcatag tcgatcccga
     1381 tgcccatctg gtgcgcggcg tcggtgagtg cgccggctcc catgcgggca cccagctgcg
     1441 cgaacgtggt gttgcaggag cgggcgaacg ccgtgcgtag cggtaccgta cccaggtcga
     1501 agagctgatc gttcggcact ctgcggtccc cgatggtggt ggccgccggg caagccaccg
     1561 gtgactccgg cgtgacggcc tgggactgca tcgccgccga cgccgtgacg atcttgaatg
     1621 tggagcctgg cggataacgc ccggtcagcg cgagcgcgcc ttgcgcgtcg gccgcgtcgt
     1681 tctgggcgac tgcgagaatc tccccggtgg agggctggat cgccacgatc atcgccgggt
     1741 ttgactctgc gtcgacagct ccttctccgg cgccttgtac gccgcgatca acggtggtgg
     1801 tcacagtcgg caccggacgt ggctgctgcg cggccagtgt ttcgacttcc tggcccagcg
     1861 tcgtcaccgt gacgatgcgc cacccggcgg ctgccgcgag ctgttcttcg accgccgtgc
     1921 ggatacccgg caccagctgg gaggcgaagt cctggtccgg tccgagcagc cgctgctggg
     1981 taggaaaggt cacccccggt agctcgtaaa tctgatcccg cactgattcg tagtcctgct
     2041 cgcgaagcac caccacggcg ccccgttgcc ctggcggagc cgcattgact gccgcaatga
     2101 tcgactgttc ggtgatctca ggatcgatcc gcgccagggc agccgccagg cgggtagcca
     2161 ccccaggcag atctgaggtt ttgctgcgat ccagcgcgac cgtgaccacg gtctgcgtgg
     2221 tgagcagcgg tgtcccgctc cgatcgacca ctgagaccgg ctcagccggc acctcgcgca
     2281 gcagcacctg ttgctgcgcg gcgagccgcg gatgcaacgc agcaggactc caccggacca
     2341 accaaccctc agcggtgtcc gcgtggatca gatcgaagct gccagcgtag ctccacttcc
     2401 ggttctgacc caggtcccag acggccgaga acgacgccga agcggtgttg tcgccagaac
     2461 gggtctggcc cagggtgagc tgcacatgct ggggtttcag ttcttcgcga acctgcccga
     2521 tcaacgcggt ggcggcgacc gggtcgtcgg tgagctgccc ggccgcagca tcgtccccgc
     2581 ggctcacggc ggcgagaaac gcagccgctg catcatccgg atcggacccg aagacaccgc
     2641 agcccgccgt caggacggcc gtcagagcca ctggcaggca cgctgccagg agctgggaaa
     2701 gtcgtcggcg cacgggggcg agtatccccc gtgaccgttc agaacagcac ggtcgcaaag
     2761 cagcccacct gggtgaaacc gatcttccgg taggtggccc tggccggctg gttgtaggca
     2821 ttgacgtaaa ggctggcgat ccggcccatc cgctgcaatc gcgtcaccac agccgcggtc
     2881 gcgccagccg cgaggttctg cccacgcctg ctcgggtgca cccatactcc ctgcaactga
     2941 cccacggtgg acgacatcac cccgatctcg gccttgaaca ccacctcatc cccctcaaac
     3001 cgggcgaagg cccggcctgc tgcgatcagc tcggcgactc gggcccggta accggcacca
     3061 ccgtcaggca gccgaggatc gacacccacc tcctcggtga acatcgccac cgcggccggc
     3121 aggtagcggt caatctcgtc ggttcgtacc agccgtacca gggggtctcc aggcaccagc
     3181 ggttgcttgt cgagcaccat cagtggctga tctggacgct cctcgcgggc cgccccccag
     3241 gtctctcgca gcctcgacca caacgggagc acctggtcgg ctggtccgac catcgatgag
     3301 caggaccggg gctgcgcagc cgcgcggtcg gcgaaggccc ggatcgccga agaccccccg
     3361 cgaagcggga ccagattggg cccggagaag cacagcccgt ccaacggacc atcgggccct
     3421 tggctgaagc cccacaattc cccgccaagc cgccatggat ccagcccacc gctctcgatc
     3481 cgagcggcca ccatgcacga cataaccgga ttgatggcca gcactgcgcg gacgccgcgc
     3541 gcatcgtggt tgtccaacag gcgcgcgctc gcgagcttca gcacgccacc tagggtgcca
     3601 gaaatagcag ccccgaacag cgacacagcg ccactcacgg ctcttcggcg aggcgctgcg
     3661 cctcctcgat gagcgtctcg acgatctgcg actcgggcac ggtcttgatc acctcgccgc
     3721 gaacgaagat ctggcccttg ccgttgcccg aggcaacacc gaggtcagcc tcgcgcgctt
     3781 caccaggtcc gttgaccacg cagcccatca cagccacccg cagcggaact tccatcccct
     3841 ccaagcccgc ggtgacctca tcggcgagct tgtagacatc gacctgggct cgcccgcatg
     3901 aggggcagga cacgatctcc agccggcggg tccgcaggtt cagggactcc aggatctgca
     3961 atccgacctt gacctcctcg accggaggtg cggacagcga gacccggatg gtgtctccga
     4021 tgccctggct cagcagtgcc ccgaatgcgg tggccgactt gatggtgccc tgaaatgccg
     4081 ggccggcctc ggtgacccca aggtgcaacg ggtagtcgca ctgcgctgcg agctgctcgt
     4141 aggcccgcac catgaccacc ggatcgtgat gcttgaccga gatcttgatg tcgtggaagt
     4201 cgtgttcggc gaacaggctc gcttcccaca acgcggattc gaccaacgct tccggagttg
     4261 ccttaccatg cttggccagg atcttgggat ccagcgaccc ggcgttgacg ccgatcc
//
LOCUS       DATADN010000246         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1084430, whole genome shotgun sequence.
ACCESSION   DATADN010000246 DATADN010000000
VERSION     DATADN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084430"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VHH53_03295"
     CDS             complement(<1..552)
                     /locus_tag="VHH53_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2289224.1"
                     /translation="MTRPLVLIAEKLAPSVLEIFDDECDVRHVDGTNRAALFEALAGA
                     EALLVRSATRVDAEALSHAHRLKVVARAGVGLDNVDVPAATERGVMVVNAPTSNIVSA
                     AEHAVALLLAVARHIPAADASLRNGQWKRSSFGGIELSGKTVGVVGLGKIGQLVAQRL
                     AAFGTELIAYDPYVSPARAAQLGI"
     gene            complement(564..1577)
                     /gene="ilvC"
                     /locus_tag="VHH53_03300"
     CDS             complement(564..1577)
                     /gene="ilvC"
                     /locus_tag="VHH53_03300"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX2289225.1"
                     /translation="MSAEIFYDSDADLSVIGTRKVAVIGYGSQGHAHALSLRDSGVDV
                     RVGLPEASKSRPKAADEGLWVVTPAQASAEADVIMILAPDTKQRTIYANDIAPNLSSG
                     DALLFGHGFNIRYDLITPPSDVDVAMVAPKGPGHLVRRQFVDGKGVPCLIAVEQDPTG
                     GAQALALSYAKAIGGTRAGVIKTTFTEETETDLFGEQTVLCGGITSLVQAGFEVLTEA
                     GYAPEVAYFECLHEVKLIVDLMYEGGIARMRYSISDTAEYGDLTRGPRVITPQVKDEM
                     RRILTEIQDGTFAQEWVAEDDNGRPNFTKLRAEGERHSIEQVGARLRGLMSWVDRPLT
                     DTA"
     gene            complement(1675..2181)
                     /gene="ilvN"
                     /locus_tag="VHH53_03305"
     CDS             complement(1675..2181)
                     /gene="ilvN"
                     /locus_tag="VHH53_03305"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX2289226.1"
                     /translation="MTTHTMSVLVENKPGVLARVAGLFSRRGFNIHSLAVGPTENPDI
                     SRMTIVVAVDELPLEQVTKQLNKLINVIKIVELDPVAAVQRELLLVKIRADTTVRSQV
                     LETVTLFRAKVVDVSPEALTIEATGTADKLDALLRMLEPFGIREMVQSGMVAMGRGPR
                     SITAGPPR"
     gene            complement(2178..>3075)
                     /locus_tag="VHH53_03310"
     CDS             complement(2178..>3075)
                     /locus_tag="VHH53_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2289227.1"
                     /translation="GPHRRDQLADHLGTVTDDRHIGPAVLGDLRRIDVPIVGDCAEVI
                     SELIAAVRAEFTSLRPDLTAWWGQLESWRTTFPLGYDPPTDGMLSPQFAIKRIGELVG
                     PDAIYTAGVGQHQMWAAQFIRYEKPGTWINSGGLGTMGFAVPAAMGAKLGRPDVEVWA
                     IDGDGCFQMTSQELATCAIEKIPIKVAVINNGSLGMVRQWQNLFYSERYSQTDLGTHS
                     RIPDFVKLAEALGCVGLRCEAEPDVDKVIEQAMEIDDHPVVIDFVVGKDAQVWPMVAA
                     GHSNDQIMAAHNIRPLFDEDGS"
BASE COUNT          507 a         1061 c          967 g          540 t
ORIGIN      
        1 gatgcctagc tgagcggccc gtgccgggga cacatagggg tcataggcga tcagctccgt
       61 tccgaaggca gccagccgct gcgccaccag ctggccgatc ttgcccaggc ccaccacgcc
      121 aacggtcttg ccggacagct cgatgccacc gaacgagctg cgcttccact gcccattccg
      181 caggctggcg tcggcagccg ggatgtgccg ggcgaccgcc agcagcagcg cgacggcgtg
      241 ctcggcagcc gagacgatgt tggaggtcgg cgcgttgacc accatcacgc cgcgctcggt
      301 ggccgccggc acgtcgacgt tgtccagccc cacaccggcc ctggcgacga ccttgagccg
      361 gtgggcgtgg ctcagcgcct cggcatccac ccgggtggcc gagcgcacca gcagcgcctc
      421 agcaccggct agggcctcga acagggccgc acgattggtg ccatcgacgt gccgaacgtc
      481 gcactcgtca tcgaagattt ccagcaccga tggtgcgagc ttctccgcga tcaggactag
      541 tggtcgggtc acgctgaccg agtctaggcg gtgtcagtga gcggccgatc aacccatgac
      601 atcagcccgc gcaaccgcgc gccgacctgc tcgatcgagt gccgctcccc ctcggcgcgc
      661 agcttggtga agttcgggcg gccgttgtcg tcctctgcga cccactcctg ggcgaaggtg
      721 ccgtcctgga tctcggtgag gatccggcgc atctcgtcct tgacctgcgg cgtgatcacc
      781 cgtgggccgc gggtgaggtc cccgtactca gcggtgtcgg agatcgagta gcgcatccgg
      841 gcgatcccgc cctcatacat caggtcgacg atgagcttga cctcgtgcag gcattcgaag
      901 tacgccacct cgggcgcgta gcccgcctcg gtgagcacct cgaacccggc ctgcaccagc
      961 gaagtgatcc cgccacagag caccgtctgt tctccgaaca gatcggtctc ggtctcctcg
     1021 gtgaaggtag tcttgatcac cccggcgcgg gtaccgccga tcgccttggc gtaggacagc
     1081 gccagcgcct gtgcaccacc ggtggggtcc tgctcgaccg cgatcaggca cggcacgccc
     1141 ttgccgtcga cgaactgcct gcgcaccagg tgccccggtc ccttaggtgc caccatcgcc
     1201 acgtccacat cagacggcgg tgtgatcagg tcgtaccgga tgttgaagcc atgcccgaac
     1261 agcaacgcgt cacccgagct caggttcggc gcgatgtcgt tggcgtagat ggtccgttgc
     1321 ttggtgtccg gcgccaggat catgatgacg tctgcctcgg ccgaagcctg cgccggcgtc
     1381 accacccaca gcccctcgtc ggccgccttc gggcgtgact tggatgcctc gggcagtccg
     1441 acgcgcacgt cgaccccgga atcgcgcagg ctcagcgcgt gcgcatgtcc ctggctgcca
     1501 tacccgatca cagccacttt ccgtgtgccg ataaccgaca gatcagcgtc actgtcgtaa
     1561 aaaatctctg cactcatcgg taactccctg ctctagctct gccgcgttct ggatgcagaa
     1621 cgcgctcaac tggggtcaaa tctgcatcca gatcgcgcgg tgttgcgtga aggtctagcg
     1681 cggcgggcca gcggtgatgg agcggggacc gcggcccatg gcgaccatgc cggactgaac
     1741 catctcgcga atgccgaagg gttcgagcat ccgaagcagg gcgtccagtt tgtctgcggt
     1801 cccggtcgcc tcgatggtca gcgcctccgg ggagacgtcg accaccttgg cccggaacag
     1861 cgtgaccgtc tcgagcacct gggagcgcac cgtggtgtcg gctcggattt tcaccagtag
     1921 cagttcccgc tgcaccgccg ccaccgggtc gagctcaacg attttgatca cattgatcag
     1981 cttgttgagc tgcttggtga cctgctccaa gggcagctcg tcgaccgcca ccacgatggt
     2041 catccgggag atgtcgggat tctcggtagg ccccaccgcc aaggagtgga tgttgaaccc
     2101 gcggcggctg aacagcccgg cgacccgggc caggacgccg ggcttgttct ccaccagcac
     2161 cgacatggtg tgcgtggtca tgatccgtcc tcatcgaaca gcgggcggat gttgtgcgca
     2221 gccatgatct ggtcattgct gtggcccgcc gcgaccatcg gccagacctg ggcgtccttg
     2281 cccaccacga agtcgatcac gaccgggtgg tcgtcgatct ccatcgcctg ctcgatgacc
     2341 ttgtcgacgt ccggctcggc ctcgcagcgc aggcccacgc aacccaatgc ctcggcgagt
     2401 ttcacgaagt ccgggattcg ggagtgggtc ccgaggtcgg tctgcgaata gcgttcggag
     2461 tagaacaggt tctgccactg ccgcaccatt cccaggctgc cgttgttgat caccgcgacc
     2521 ttgatcggaa tcttctcgat ggcgcacgtc gccagctcct gactggtcat ctggaagcag
     2581 ccgtcgccgt caatggccca gacctcgacg tcgggcctgc cgagcttggc ccccatcgcc
     2641 gccggcaccg cgaaacccat cgtgccaagg cccccggagt tgatccaggt acccggcttc
     2701 tcgtatctga tgaactgcgc cgcccacatc tggtgctgac ccaccccagc ggtgtagatc
     2761 gcgtccgggc cgaccagttc accgatccgc ttgatcgcga actgtggaga cagcatgccg
     2821 tccgtcggcg ggtcataacc cagcgggaac gtcgtgcgcc agctttccaa ctggccccac
     2881 cacgcggtga gatccggccg tagggaggtg aactcggccc gcaccgccgc gatcagctcg
     2941 ctgatcacct cggcacagtc accgacgatc ggcacatcga tcctgcggag atctccaaga
     3001 accgccgggc cgatgtgccg atcgtcggtg actgtgccga ggtgatcagc gagctgatcg
     3061 cggcggtgcg ggccg
//
LOCUS       DATADN010000247         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1084467, whole genome shotgun sequence.
ACCESSION   DATADN010000247 DATADN010000000
VERSION     DATADN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084467"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VHH53_03315"
     CDS             complement(<1..233)
                     /locus_tag="VHH53_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289228.1"
                     /translation="MTHPVRIQPAEQAYRALNNPVQPAACGPDVLATLATVNVDGDVG
                     KELLMPPRGLFVGVTTLDVVQRVARRPGVDEKVV"
     gene            232..1695
                     /locus_tag="VHH53_03320"
     CDS             232..1695
                     /locus_tag="VHH53_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB1 family IMP dehydrogenase-related protein"
                     /protein_id="HEX2289229.1"
                     /translation="MEPGNVARVHFLGDRRPVHDLTYDDVFLMPSHSEVVSRFDADLS
                     TVDGSGTTIPLVVANMTAVAGRRMAETVARRGGLVVLPQDVDPEVVADTVAWVKSRHL
                     VLDTALTLGPQDAVADAANLLPKRAHRAVVVLDGASHPVGLVTEAELTGVDRFARLTD
                     VAQTDLVVVSADTEPRKVFEQLHHRRQELALAVDAGGRLVGVLTPTGALRAELYSPAL
                     DAGGRLRVAAALGVTGDVAAKTEALIAAGVDVLVVDTAHGHQAKMLDALCTVRSRQPD
                     VPVVAGNVVSAQGTCDLLKAGADIVKVGVGPGAMCTTRMLTGVGRPQFSAVAECAAAA
                     RAAGGRVWADGGIRHPRDVALALAAGAANVMIGSWLAGTYESPGDLKYDENGRPYKES
                     YGMASKRAVAARTTHDDTFDRARKALFEEGISTARMYLDPSRPGVEDLIDHICAGVRS
                     ACTYAGARTLPELSERAMIGVQSAAGFAEGRPLPAGW"
     gene            complement(1697..>2485)
                     /locus_tag="VHH53_03325"
     CDS             complement(1697..>2485)
                     /locus_tag="VHH53_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX2289230.1"
                     /translation="RRCWGMVLGVPGSVTFDAVESGVDVVEVLARGAEPGTKSTDLRA
                     VEGPAARLEVVRLLSDAIVVLPGGIDVLADLLALLSEQALGLSDKPCGVLDPTGLLDP
                     LAEQLDALDRAGLLAAPLLRADDPATLLDRLAAWRPNGGGELRDEVAWLRVSDSSLTL
                     LPTPSGLSLPGGPREPGERGAVALCRLLEQRWSVRLSPERLHPAAALVVPEPDGGWRR
                     VSCYRADGPQPVVPGAVSHPLGETTACDPTAATLQELLRQGRVL"
BASE COUNT          390 a          886 c          847 g          362 t
ORIGIN      
        1 acgaccttct cgtcgacacc gggccgccga gctacccgct gcaccacgtc cagggtggtc
       61 actccaacaa acagcccgcg aggtggcatc agcaactcct tgcccacgtc cccatcaacg
      121 ttcacggtgg ccagtgtggc gagcacgtct ggaccgcaag cagcgggctg gacggggtta
      181 ttgagtgccc ggtaagcctg ctcagccggc tgaatgcgga ccggatgggt catggagccg
      241 ggtaacgtcg ctagggtgca tttccttggt gatcgccgac ccgttcacga cctcacgtat
      301 gacgatgtct tcctgatgcc gtcgcactca gaggtggtgt cccgtttcga tgcggatcta
      361 tccaccgtgg acggatcggg gaccaccatc ccgcttgtgg tggccaacat gacagcggta
      421 gcgggacgcc ggatggccga gacggtggcc cgtcgtggcg ggctggtggt actgccccag
      481 gacgtggatc cggaggtggt cgccgacacg gtggcgtggg tgaaatcgcg gcacctggtc
      541 ctggataccg ccctcacgct gggaccgcag gacgccgtcg ccgatgcggc gaacctgctg
      601 ccaaagcggg cccaccgcgc agtggtggtg ctcgacggcg caagccaccc ggtaggcctg
      661 gtcaccgagg ccgaactcac cggagtggac cgcttcgccc ggctgaccga cgtggcacag
      721 accgacctgg tagtggtatc cgcagacacc gaaccacgca aggtcttcga gcagctgcat
      781 caccgccgcc aggaactggc gctcgcggtg gacgccggcg gccggctggt gggggtgctg
      841 actcccaccg gagcgttgcg ggccgagctg tattcccccg ccctggacgc cgggggccgg
      901 ctgcgggtcg ctgcggcact cggggtgacc ggcgacgtcg cggccaagac cgaggcactg
      961 atcgccgcgg gcgtcgacgt gctggtcgtc gacaccgcac acggccacca ggccaagatg
     1021 ctcgacgcgc tgtgcacggt ccgctcacgc cagccggatg tgccggtggt ggccgggaat
     1081 gtggtctccg cccagggcac ttgtgacctg ctgaaggccg gtgcggacat cgtcaaggtg
     1141 ggggttgggc cgggcgccat gtgcaccacc cggatgctca ccggagtcgg ccggccgcag
     1201 ttctccgcgg tagctgaatg tgccgcggcg gctcgcgcgg ctggtggccg tgtctgggcc
     1261 gacggcggta tccggcaccc acgagacgtc gcgctggcac tggccgccgg ggcagcgaat
     1321 gtgatgatcg gctcctggtt ggcgggcacc tacgagtcgc cgggtgacct caagtacgac
     1381 gagaacggcc gtccctacaa ggagtcttac gggatggcct ccaagcgtgc cgttgcggcc
     1441 cgcaccactc acgacgacac cttcgaccgg gctcgcaagg cgctgttcga ggaaggcatc
     1501 tccactgccc ggatgtacct cgatccgagc cggccggggg tggaggacct catcgatcac
     1561 atttgcgccg gggtgcgcag cgcctgcacc tacgccggtg cccggacgct gccggagctg
     1621 tccgagcgcg cgatgatcgg agtgcagtcc gccgccgggt tcgccgaggg ccgcccgcta
     1681 ccggccggct ggtgacctag agcacccggc cctggcggag tagttcctgc aacgtcgcag
     1741 ccgtcggatc gcaagcggtg gtctccccca gcggatgcga caccgcaccc ggcaccaccg
     1801 gttgtgggcc gtcggcccgg taacaactca ctcgccgcca cccgccgtcc ggttccggca
     1861 cgaccagcgc cgccgccgga tgcagccgtt ctgggctcaa ccgcaccgac cagcgctgct
     1921 ccagcaaccg gcacagcgcc accgctcccc gctcccccgg ctcacgcggt ccaccgggca
     1981 gggacagccc cgacggggtg ggcagcaggg tcaggctgga atcgctgacc cgtaaccagg
     2041 cgacctcgtc gcgcagctcg ccgccaccgt tgggccgcca cgccgcgagc cggtccaaca
     2101 gcgtggctgg gtcatctgcc cgcagcagcg gtgcggctag caggccggcg cggtccagcg
     2161 cgtcgagctg ctcggccagc ggatcgagca acccggtcgg gtccagcacg ccacacggtt
     2221 tatcactgag tcccagtgcc tgctcactga gcagagcaag caggtcggcc agcacgtcga
     2281 tcccacccgg cagcaccacg atggcgtcgg acagcagccg caccacctcc agccgggcag
     2341 ccggcccttc cactgctcgt agatcagtgg acttggtacc cggctccgcg ccgcgcgcca
     2401 gcacctccac gacgtctact cccgactcga cagcgtcgaa ggtgaccgac cccggcactc
     2461 cgagcaccat gccccagcaa cgccg
//
LOCUS       DATADN010000248         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_627250, whole genome shotgun sequence.
ACCESSION   DATADN010000248 DATADN010000000
VERSION     DATADN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627250"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="rpoA"
                     /locus_tag="VHH53_03330"
     CDS             complement(<1..582)
                     /gene="rpoA"
                     /locus_tag="VHH53_03330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009978953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX2289231.1"
                     /translation="MLITQRPNLSEEPVVDTRSRFVIEPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSIRIDGVLHEFTTVPGVKEDVTDIILNLKELVVSSEEDEPVTMYLRKQGP
                     GDVTAGDIVPPAGVTVYNPDLHIATLNGKGKLEIELVVERGRGYVPAVQNKASGAEIG
                     RIPVDSIYSPVMRVTYKVEATRVEQRTDFDKLIL"
     gene            complement(723..1328)
                     /gene="rpsD"
                     /locus_tag="VHH53_03335"
     CDS             complement(723..1328)
                     /gene="rpsD"
                     /locus_tag="VHH53_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX2289232.1"
                     /translation="MARYTGPATRKSRRLKTDLVGGDQAFERRPYPPGQHGRARIKES
                     EYLLQMQEKQKARFTYGVMEKQFHRYYEEANRRPGKTGDTLLQILESRLDNVVYRAGL
                     ARTRRQARQLVSHGHFLVNGKKVDVPSFRVSRFDIIDVKPKSIPTLPFVVAKETLGDR
                     PVPAWLQVVPSSLRVLVHRLPERAQIDVPLQEQLIVELYSK"
     gene            complement(1350..>1629)
                     /gene="rpsK"
                     /locus_tag="VHH53_03340"
     CDS             complement(1350..>1629)
                     /gene="rpsK"
                     /locus_tag="VHH53_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX2289233.1"
                     /translation="TGAVIAWASAGHVGFKGSRKSTPFAAQMAAENAARKAAEHGMKK
                     VDVFVKGPGSGRETAIRSLQAAGLEVGTIQDVTPQPHNGCRPPKRRRV"
BASE COUNT          266 a          522 c          529 g          312 t
ORIGIN      
        1 caagatcagc ttgtcgaagt cggtgcgctg ctcgacccgg gtagcctcga ccttgtaggt
       61 caccctcatc accggcgagt agatggagtc caccggaatc cggccgatct ccgcacccga
      121 ggccttgttc tgcacggcag gcacgtagcc gcggccccgc tcgacgacca gctcaatctc
      181 cagcttgccc ttcccgttca gggtggcgat gtggagatca gggttgtaca ccgtgacgcc
      241 ggcaggcggc acgatgtcgc ccgcggtgac gtcacccggg ccctgcttgc gcaggtacat
      301 cgtcaccggc tcgtcctctt cggaactgac cacgagttcc ttgaggttga ggatgatgtc
      361 ggtgacgtcc tccttgacac ccggaacggt ggtgaactca tgcagcacac catcgatgcg
      421 gatgctggtg accgccgctc ccgggatgga cgagagcaag gtccggcgca gtgagttgcc
      481 cagggtgtaa ccgaagcccg gttccagcgg ctcgatgacg aaccgggacc gggtgtcgac
      541 gaccggctcc tcgctgaggt tgggtcgctg agtgatcagc atagtgatgc tcctccttgg
      601 cgcccgctat ttgacgccat catggatctt gaattctcat agccttcccc gtcatggagc
      661 catgtcgggg tcgtagaggg gtgacgcacc ccgatatgtc tccatatcgg ggtgcagtca
      721 ggttacttgg agtagagctc gacgatcagc tgctcctgca gtggcacgtc gatctgcgcc
      781 cgctccggca gccggtgcac cagcactcgc agcgaagacg ggacgacctg cagccacgcc
      841 ggcacggggc ggtctccgag ggtctccttg gccaccacga aaggtagcgt cgggatcgac
      901 ttcggtttga catcgatgat gtcgaacctg gacacccgga aactgggaac atcgaccttc
      961 ttgccattga ccaggaaatg cccgtgggac accagctggc gggcctggcg acgagtacgg
     1021 gccaggcccg ctcgataaac gacgttgtcc agccgggact ccaggatctg cagcaaggtg
     1081 tcaccggtct tgccggggcg gcggttggcc tcctcgtagt accggtggaa ctgcttttcc
     1141 atgacgccgt aggtgaagcg ggctttctgc ttttcctgca tctgcaggag gtactcgctc
     1201 tccttgattc gcgcacggcc gtgctggccg ggcggatacg gacggcgctc gaacgcctga
     1261 tcgccaccga ccagatcggt cttgagccgg cgggacttgc gggttgcggg tccggtgtac
     1321 cgagccatct gtgctatccc tcccgcgcct tagacccgac gccgtttcgg cggccggcag
     1381 ccgttgtgcg gctgcggggt cacgtcctgg atggtgccaa cctcaaggcc ggcggcctgc
     1441 agcgaccgga tcgctgtctc ccggcccgaa cccgggccct tcacgaagac gtcgaccttt
     1501 ttcataccgt gctcagcggc cttgcgggcc gcgttctcgg cggccatctg ggcggcgaac
     1561 ggagtggact tgcgcgagcc tttgaagccg acgtggcccg cggaggccca ggcgatcacc
     1621 gcaccagtg
//
LOCUS       DATADN010000249         3079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_22651, whole genome shotgun sequence.
ACCESSION   DATADN010000249 DATADN010000000
VERSION     DATADN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22651"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VHH53_03345"
     CDS             <1..418
                     /locus_tag="VHH53_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="HEX2289234.1"
                     /translation="GPVRVCVAAARDHGEKVLDPLYTAMGRRIHNEGNKDVDEVIAAA
                     LAEVGLPAELAAAARSTEYDEELRASHEAGMGPVGLNVGTPTIHVDGVAFFGPVLSRI
                     PRGEDAGKIFDGALLLASYPHFFELKRTRHEKPDFS"
     gene            complement(479..1270)
                     /locus_tag="VHH53_03350"
     CDS             complement(479..1270)
                     /locus_tag="VHH53_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2289235.1"
                     /translation="MTARWVELADGVLVRRYAELDLSVGLVIGDQGCLVVDTRSDPTQ
                     GAELQDAIRQVTRLPCSVVITHAHFDHCLGTAAFLPATVWAHPRCRDDLARGGAAQHA
                     EALAWGRTHRALVPPVVPNHLVAQQAEVELGRRQVLLTYAGRGHTDHDIAAWSPDTGV
                     LFAGDLIEQGADPDFTDAYPLDWPATVTGLLQLGPHTVVPGHGDPVDAQFVAAQRDDL
                     ATLAAICRAVQTGELDTTSALEASPFGRTATLAALTRLDPPDMET"
     gene            complement(1332..2066)
                     /locus_tag="VHH53_03355"
     CDS             complement(1332..2066)
                     /locus_tag="VHH53_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX2289236.1"
                     /translation="MRREHIQDGYDVRLEWGREGAALLGAHCAVVVIIDVLSFCTAVD
                     VAVERGAAVLPLPWGDERALDAAQRAGALLAGPRGSQGFTLSPTSLQTVPAGVGLALP
                     SPNGATLCAIAQRTGAHVLTGCLRNAAAIARTAHQLARGGPIGLVPAGERWELPGEPM
                     RPALEDTVGAGAIANVLSGAFSPEAELAAAGFGAARSRGLHGVLAASASGRELVADGF
                     AADVTLAAAYNTSEIAPQLQGGVLTA"
     gene            2116..2589
                     /locus_tag="VHH53_03360"
     CDS             2116..2589
                     /locus_tag="VHH53_03360"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEX2289237.1"
                     /translation="MRVYLASDHAGFELKNFLLQHLKSLGHDVVDVGAEAFDADDDYP
                     PFCIEAARRVVANAGSLGVVIGGSGNGEQISANKVPGCRAALAWSTDTAQLAREHNDA
                     QVVGIGARMHSPAEAAEIVETFLNTPFSGGERHSRRIRQISDFESTGEPPPLSRH"
     gene            2792..2923
                     /locus_tag="VHH53_03365"
     CDS             2792..2923
                     /locus_tag="VHH53_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289238.1"
                     /translation="MDVRPDIFDVGDAYPPSYIETAHRIRLIADFEAAGHAPSISAG"
     gene            2931..>3079
                     /locus_tag="VHH53_03370"
     CDS             2931..>3079
                     /locus_tag="VHH53_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HEX2289239.1"
                     /translation="MPEGHTTHRLAGAHQRCYGGSPVAVSSPQGRFAAAAAQLDGRVL
                     WRAEA"
BASE COUNT          508 a         1018 c         1041 g          512 t
ORIGIN      
        1 gggaccggtt cgggtgtgcg tcgccgcagc ccgagatcat ggcgagaagg tgctcgatcc
       61 gctctacacg gcgatgggta ggcggatcca caatgagggc aacaaggacg tcgatgaggt
      121 gatcgccgcc gcactagctg aggtggggtt gcctgcggag ctggccgcag ctgcgcgcag
      181 taccgagtac gacgaggagc tgcgggccag tcacgaggcc ggcatgggtc cagtcgggct
      241 gaatgtcggg acccccacca ttcacgtaga cggggtggcc tttttcggcc cggtgctgtc
      301 ccggataccg cgtggcgagg atgccggcaa gatcttcgac ggcgctctgc tgctggccag
      361 ttacccgcac ttcttcgagc tcaagcgcac ccggcacgag aagcccgact tctcctgacc
      421 cactgccgcg taccccgaca tggagacata ttgggctatt ttgaccgtca atagcccgtt
      481 atgtctccat gtcgggtgga tcgagcctgg tgagtgcggc aagggtggca gtgcggccaa
      541 atggcgatgc ctcgagcgcg cttgttgtgt cgagctcgcc ggtctgcacc gcacggcata
      601 ttgcggccag cgtcgccagg tcgtcgcgtt gtgcggccac gaactgagcg tcgaccgggt
      661 cgccgtgccc agggaccacg gtgtgcgggc ccagttgtag cagtccggtc accgtggcgg
      721 gccagtccag ggggtaggca tcggtgaagt ccggatcggc gccctgctcg atgagatccc
      781 cggcgaagag cacacccgtg tcgggcgacc atgccgcgat gtcatggtcg gtgtgtccac
      841 gtccggcata ggtcaacagc acctgtcgac gaccgagctc gacttcggct tgctgggcga
      901 ccaggtgatt gggcaccaca ggaggcacca gcgcgcggtg tgtgcgaccc caggccaatg
      961 cctcggcgtg ctgcgccgcg ccgcccctgg cgaggtcgtc ccggcaccga gggtgtgccc
     1021 ataccgtggc cggcaggaag gccgcggtgc ccaggcagtg atcgaaatgc gcgtgggtaa
     1081 tcaccacgct gcaaggcaag cgggtgactt ggcggatcgc atcctgcagc tcagcgccct
     1141 gagttggatc gctgcgggtg tccacgacca ggcagccctg gtccccgatc accagcccca
     1201 ctgacagatc cagttcggcg taccgccgca cgagcacccc gtcggccaac tcaacccacc
     1261 gcgccgtcac acatgctcct cacacgctgc cacgccccct tcccgacatg gagacatatc
     1321 ggggaagagt gtcatgcggt cagcactcca ccttgcagtt gtggtgcgat ttcgctggtg
     1381 ttgtacgccg cggccagcgt gacatcggcg gcgaatccgt cggcgaccag ttcccgcccg
     1441 gaggcgctgg ccgcgaggac gccgtgcaga ccgcgcgagc gtgccgcgcc gaaccccgcc
     1501 gcggccagct ccgcctccgg tgaaaacgcc ccggatagca cgttagcgat cgcgcccgcc
     1561 ccgacagtgt cctcgagcgc gggccgcatc ggttccccag gcagttccca ccgctcgccg
     1621 gccggtacca acccgatcgg accgccgcga gccagctgat gcgcggtgcg ggcaatcgcg
     1681 gccgcgttac gaaggcaccc ggtgagcacg tgggcgccgg tacgctgcgc gatcgcgcac
     1741 agcgtcgcac cattgggcga gggcagtgcc agccccaccc cggcgggcac ggtttgcaac
     1801 gaggtcggcg acagcgtaaa accctgcgaa ccgcggggac cggccagtaa cgctccggca
     1861 cgctgggcgg cgtcaagggc gcgctcgtcg ccccagggca gcggtagcac ggcggcccca
     1921 cgttcgacgg ctacgtccac ggccgtgcag aatgagagta cgtctatgat caccaccacc
     1981 gcgcagtgtg ctccgagcag agctgctcct tcccgacccc attccagccg gacgtcgtag
     2041 ccgtcctgga tgtgctcccg gcgcaaactg tgccctcctc acccggcgat ccgcgatatg
     2101 gcaatgttgc acaccgtgcg cgtttacctc gcttccgacc acgctggctt cgagctcaag
     2161 aatttcctgc tgcagcacct gaaaagtctc gggcacgacg tggtggacgt cggtgctgag
     2221 gccttcgacg cagacgacga ctatccgccg ttctgtatcg aggcggcccg ccgggtggtg
     2281 gccaacgcag gcagcctcgg tgtggtgatc ggcgggtcgg gcaacggcga gcagatctcc
     2341 gccaacaagg ttccgggatg ccgtgccgcg ctggcctgga gcaccgacac ggctcagctg
     2401 gcccgcgaac acaacgacgc tcaggtggtg gggatcggcg cccggatgca cagtcccgct
     2461 gaagcagccg agatcgtcga gacttttctc aacacaccgt tctcaggagg cgagcggcac
     2521 tcccgccgga tcaggcagat ctcggacttc gagtccaccg gggagccacc cccgctgtcc
     2581 aggcactgag ctggcgatgc cggagggtca tacgacgcgc cggttggctc ggcggcatga
     2641 accggagaca tggagacata tcgggatcgg gacggtgcgg atgcgcggtg tggcactgtt
     2701 gcacactgtg cgcgtctatt tcggttcgga tcatgctggg tttgagctta gggcttttgg
     2761 tgaggcactt ggtagcctct ggcacgatgt ggtggacgtc aggcctgaca tcttcgatgt
     2821 tggcgatgcc tacccaccgt cctacatcga gaccgcccac cggatccggc tgatcgcgga
     2881 cttcgaggcc gccgggcacg caccgtcgat ctctgcgggc tgactctccg atgccggagg
     2941 gtcacacgac acaccgactg gcaggtgcgc accagcgttg ttatggaggg tcgccggttg
     3001 cggtgtccag tccgcagggt cgtttcgcgg cggccgctgc acaactcgat ggtcgggtat
     3061 tgtggcgtgc cgaagcgca
//
LOCUS       DATADN010000250         4336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_480863, whole genome shotgun sequence.
ACCESSION   DATADN010000250 DATADN010000000
VERSION     DATADN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480863"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1856
                     /locus_tag="VHH53_03375"
     CDS             324..1856
                     /locus_tag="VHH53_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289240.1"
                     /translation="MVRPHSPPDLAVSTPSPAALWIFRQVAGAPSAPLTAAIVGPGGT
                     GKSVLLDALAREYQKAGALVMRGNRAGLRLDTVDPDEPVLVDDAHQLDQATLEGLRTF
                     AEGETVRLVVAYRPWPRSRELSALGACLARRRSPVVVGHLDRSTVGARIAARVGCTPP
                     DSLIDLVHEQSGGLPSLVNLVTQALQDTGRFDPRRPDKFRCPERVNVSAGLAERLRYQ
                     LEALDPAIYGLLQAMAVGAALDADVLSPLLDADPASLTDTVEAAMATGLLTNGGELIP
                     FIRNIVLRLMPMMRSRAMQRQLATIQLDRGGSVLAAGRQLLGTGASGNRVAAVLERAG
                     DEALRQSPALAAELYAGTIDAGASPSALAARHAQAVALAGDLDQALRLADQVVSDQSA
                     ADRKRGISVVAAILAHRGLPAHSAQLYCSLPADATGGAALAVPALIGIGELAQARAVL
                     DAADAGRGATLMAGAEILMARGMLATVTGASSEALSQLARATVLLERRVKPCCCRIRR
                     QR"
     gene            1826..2965
                     /locus_tag="VHH53_03380"
     CDS             1826..2965
                     /locus_tag="VHH53_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2289241.1"
                     /translation="MLLPDTPAALTAVVALQCGELTVADSTLRRAVAAKLGGRPAHPR
                     HLLLHGWMAMVRGKLEVAHRTLERVTRQFTALEPRDEVVAAALAVGLARRKGDAAALR
                     SAWRRARCALVRHPIDLYMLQPLGELAIAAARSNELESVAPHLKEAENLLDKLGNPVL
                     WAVPLHWYQLHAAIVAEQLPEAQRHAAALTDAASSSPYGAVLAAAAHSWLQVLSGDID
                     PEIVQIAARRLHGVGLAWDAAQLPAQAATRTIDRRAMSALLTCARALNGNLPSGPDSV
                     PGSGDELGPETCSQGRSMNPEPGEATVTMAAGSGPVEGQSVLSERELEVGQLILAGLT
                     HKQVGERLFISAKTVEHHMARIRQRLGVGTRNELFDQLRAIIEGS"
     gene            complement(2998..>4336)
                     /locus_tag="VHH53_03385"
     CDS             complement(2998..>4336)
                     /locus_tag="VHH53_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX2289242.1"
                     /translation="STLLNALVGEQVAPTDAGECTRVVTWYCDGHSPKIVMHPKEGPP
                     TPLPVNRQDGALAIDLQGTSAEALNRLVVHWPSQSLRTATLIDTPGIASVSADNSQRT
                     LTFLTPEDDSPAEADAVIYLMRHLHATDAKFLESFRDQGVGRATSVNTVAVLSRADEI
                     GGGRVDAMFSAKTIAQRYRKDPTLRALCQGVVPVAGLLAQTGRTMRQVEFEALAALSR
                     APREEVDGLLLSSDRFLRADSAAVTTIQPVESPEIRHGLLHRFGLFGLRLSVMLIRQG
                     TDTPGALAEELVRRSGLDELRELLNTQFTERRDLLKARSALLALDSVLHGDTRADTGP
                     LLGDVERILAGAHEFAELRLLSALRSGAVNLPKAELDEAERLLGGSGAAPEVRLGQPP
                     GSKADELRQAALEALTRWHRRAENPLSGRATADACRVVVRSCEGMMARLATGS"
BASE COUNT          689 a         1488 c         1459 g          700 t
ORIGIN      
        1 accgtctgca cccacaaaga gaaccgaggg caccgatggc atccggctac ccagagctac
       61 cgcttcggtc ttgtcctcat cccgccgcca gatggctgcc gccgtgaagc tcgtcccgac
      121 gtcgaccccc agtgcgtaac tcaacgatcc acgacctctc gaccacggtt aagcccaccg
      181 aatggcggtg cgttggttcc atcggtccac aacagtgagt tgttaaccgg ttacgccgtg
      241 atccggggag cgtaacgtta cgcaacctga tagcgatcga ggcgttagtg attcgtacga
      301 gggacagtct cgagagaggc agcatggtcc gaccgcactc cccgcctgat ctcgcggtct
      361 ccaccccgtc gccagcagcg ctctggatct ttcgccaggt cgccggcgct ccatcggctc
      421 cgctcactgc ggcgatagtc ggcccgggcg gtaccgggaa gtcagtgcta ctcgacgcgc
      481 tggcgcgcga gtatcagaag gctggcgccc tggtgatgcg cggcaaccgt gctggcctgc
      541 gactggatac tgtcgatccg gatgaacccg ttctggtgga cgatgcgcac cagctggacc
      601 aggcgacgct cgaagggctg cgcacgttcg ccgaagggga aaccgtccgg ctcgtcgtcg
      661 cctaccgacc gtggccgcga tcccgcgagc tctcggcgct cggcgcgtgc ctcgctcgcc
      721 gtcgatcacc agtggtggtg gggcacctcg atcgcagtac cgtcggggcc cgcatcgcag
      781 cacgagtcgg gtgcactccc cctgactcgc tgatcgacct cgttcacgaa cagagtggcg
      841 ggctgccgtc gctggtcaac cttgtcacgc aggcactcca agacaccggc agattcgatc
      901 ctcggcgccc ggacaagttc cggtgccccg agcgagtcaa cgtctccgcg ggcttggccg
      961 aacggctgcg ctaccagttg gaggcactcg atcccgcgat ctacgggctg ttgcaggcga
     1021 tggccgtcgg cgcagccctg gatgccgacg tactgagccc gttgctcgac gccgatccgg
     1081 cttccttgac cgacacggtg gaagccgcga tggcaacggg cctgctcacc aacggcggtg
     1141 agttgatccc gttcatccgc aacatcgtgc tgcggttgat gccgatgatg cgaagccggg
     1201 cgatgcagcg gcagttagcc accattcagc tcgaccgggg tgggtccgtg ctcgcggctg
     1261 gacgccagct cctgggcact ggcgcaagcg gtaatcgagt ggctgcggtc ctggaacggg
     1321 ccggcgacga ggcgctgcgg cagtccccgg cgctggccgc cgaactgtac gcgggaacga
     1381 tcgacgctgg agcctcgcca agtgcactgg cggcgcgcca cgcccaggcg gtggcgctcg
     1441 ccggtgacct cgatcaggcg cttcgactgg ctgatcaggt tgtttctgac cagtccgctg
     1501 ccgatcggaa gcgcggcatc tcggtggtgg ccgcgatcct cgcgcatcgg ggcctaccgg
     1561 ctcacagtgc ccagctgtac tgcagcctgc ctgccgatgc gaccggtggt gccgcgcttg
     1621 ccgtgcccgc attgatcggt atcggagagc tcgcccaagc tcgtgcggtg ctcgacgcgg
     1681 cggacgccgg ccggggcgcc accctgatgg ccggcgcaga gatcctgatg gcacgcggca
     1741 tgctcgccac cgtcactggt gcatccagtg aggcactgtc acagcttgcc agggccacgg
     1801 tgctgctcga gcggcgggtg aaaccgtgct gctgccggat acgccggcag cgctgaccgc
     1861 agtggtcgcg ctgcagtgcg gtgagctgac cgtcgcggac tccacgctgc ggcgcgccgt
     1921 cgccgccaag ctcggtggcc gtccagcgca tccccgacac ctgctgctgc acggctggat
     1981 ggcgatggtc cgcggcaagc tggaggtggc gcaccgcacc ctggagcggg tgacccgcca
     2041 gttcactgcg ctggaaccgc gtgacgaggt ggttgctgct gcgctggcag tgggcttggc
     2101 tcgccgaaaa ggtgacgctg ccgcgctgcg ttcggcgtgg cgacgggcac gctgcgccct
     2161 cgtccggcac ccgatcgatc tctacatgct gcagccactg ggtgagctcg ccatcgccgc
     2221 cgcccggagc aacgagctgg agtcagtcgc gccacacctc aaagaggccg agaacctgct
     2281 cgacaagctc ggcaacccgg tgctgtgggc agtgccgctg cactggtacc agctgcacgc
     2341 tgccatcgtc gccgagcaac tcccggaagc acagcggcac gccgccgcgc tgaccgacgc
     2401 tgcatcaagc agcccctacg gagctgtgct ggccgctgcg gcgcacagct ggctgcaggt
     2461 gctgtccggc gacatcgatc cggagatagt gcagatcgcg gcacggcgcc tgcacggtgt
     2521 cggcctggcg tgggacgccg ctcagctgcc ggcgcaggcc gcgacgcgga ccattgatcg
     2581 ccgagcgatg tcggccttgc tcacctgcgc ccgtgccctg aatggcaacc tgcccagtgg
     2641 cccggacagc gtcccggggt cgggtgacga gttgggccct gagacctgct cgcagggtcg
     2701 aagcatgaac cctgagcctg gcgaggccac agtgaccatg gctgcgggca gtgggccggt
     2761 ggagggccaa tcggtcctca gcgagcggga actggaggtg ggccagctca tcctcgctgg
     2821 cctgacccac aagcaggtcg gggagcggct gttcatctcg gcgaaaaccg tcgagcatca
     2881 catggccagg atccgtcagc ggctcggcgt cggtacccgc aatgagctat tcgaccagct
     2941 gcgggcaatc atcgaggggt cgtgactggg taacagtgtt atagcactca atgccactca
     3001 cgacccagtg gcgagccggg ccatcatgcc ctcgcaactg cgcaccacca cccggcacgc
     3061 atcggccgtc gcccgaccgg acagcgggtt ctccgcccgc cggtgccatc gggtgagggc
     3121 ttcgagggca gcctgtcgaa gctcgtcggc cttggagccg gggggctgac ccagccgcac
     3181 ctctggtgct gcacctgatc caccgagcag ccgttccgct tcgtccaact ccgccttcgg
     3241 caggttgacc gccccagacc gcagcgccga cagcaggcgc agctcggcga actcgtgcgc
     3301 cccggcgagg atgcgctcca catccccgag taacggtccg gtgtctgcac gcgtgtcgcc
     3361 atgcaacacg ctgtccagcg ccagcagtgc cgaacgggcc ttgagcaggt cacgacgctc
     3421 ggtgaattgc gtgttgagga gttcccgcaa ctcgtccagc ccacttcggc ggaccagctc
     3481 ctcggcgagg gcgcccggcg tgtcggtgcc ttgccggatg agcatcaccg acagccggag
     3541 tccgaacaga ccgaaacggt gcaacaaccc gtgcctgatc tcgggactct ccaccggctg
     3601 gatggtggta accgcggcgg aatcggcgcg caagaagcgg tcgctggata gcagcaaccc
     3661 gtcgacctcc tcccgcggtg cccgggacag tgcagccagc gcctcgaatt cgacctgccg
     3721 catcgtgcgt ccggtttggg ccagcaagcc cgccaccggc acgacgccct gacacagcgc
     3781 ccgcagcgtg gggtctttgc gataacgctg cgcgatggtc ttcgccgaga acatcgcatc
     3841 gacccggccg ccgccgatct cgtcggcccg cgagagcacc gcgacggtgt tcaccgatgt
     3901 cgcgcgacct actccctggt cacggaacga ctccaggaac ttggcgtccg tggcgtgcag
     3961 gtgacgcatc aggtagatca ccgcgtcggc ctcagccgga gagtcgtcct cgggggtgag
     4021 gaaggtaagc gtgcgttgcg agttgtcggc cgacaccgac gcgatacccg gcgtgtcgat
     4081 caaggtggcg gtgcgcaggc tctgcgaggg ccaatgcacc accagccggt tcagcgcctc
     4141 ggcggacgtg ccctgcagat cgatggccag tgccccgtcc tgccggttca ccggtagggg
     4201 ggtgggcgga ccctccttgg gatgcatcac gatcttggga ctgtgcccgt cgcagtacca
     4261 ggtgacgacc cgggtgcatt ccccggcgtc ggtcggagcg acctgctccc ctactagcgc
     4321 gttgagcagg gtcgac
//
LOCUS       DATADN010000251         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_332302, whole genome shotgun sequence.
ACCESSION   DATADN010000251 DATADN010000000
VERSION     DATADN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332302"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..1369
                     /locus_tag="VHH53_03390"
     CDS             383..1369
                     /locus_tag="VHH53_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289243.1"
                     /translation="MWRSASCLQLGLDPQRAMVLDGLPEPLATLLKRMDGVRSTAELI
                     ATAETAGSPAGDALAMLTDLHHSGLVQDASAADDGPPGWHRVALATEATSWSVHTTCA
                     TRRVLRQRREAAVRVVGSGRVAVALATLLAAAGVGQVAVEATGTVAAADIGTGYLPDD
                     VGRTRAAAATDAVRRCAPEATGTLRHQPDLIVLCDVPVPEPVMVTELLAAGISHLIGY
                     AHEGTAVVGPLVCPGRSSCLHCAELHRADSDLAWPKLAAQLAGRVQSAGLACTQLAAA
                     LTAEQVLAALAGSRAGLPVPPTWGGTLEVDPVRGTLHRHPRPVHPRCGCGAR"
     gene            1437..>1705
                     /locus_tag="VHH53_03395"
     CDS             1437..>1705
                     /locus_tag="VHH53_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX2289244.1"
                     /translation="MTDIPRKAAARTARLASLPLGVAGRVAAGWGKRLAGQDARDVNA
                     ELSARTAEQLFAVLGELKGGAMKFGQALSVFEAAIPDALAEPYREA"
BASE COUNT          248 a          609 c          597 g          251 t
ORIGIN      
        1 aagtcaggcc gccgacctgg acgatccgat cgcggcgttg gagcgcaccg ccgccgctga
       61 cgcccctcgc gaggattctc ccgagtctcc cgagtctccc gggtctcccg agggccccgg
      121 tgcctccggc cccgagcctc ccgacgacca acctcagcgc cctccggagt gatcgtcgtc
      181 gtctcattgt ttccagggct ggtgcaggcc gccgcctgtg cacagctgtg gataaaccgt
      241 ggataaccgg tcggggctgt ggacagccgt ttgagctacc gcgtcgggcg caccaagatg
      301 ccgggacatg agcctgccta agacccccgt catgccgcct tctctgcctg agcggctacg
      361 actcgcgccg ggccgagcgg ccgtgtggcg gtccgccagc tgcctgcagc tcgggttgga
      421 cccgcaacgc gccatggttc tcgacggcct gccggaaccg ctcgcgacgc tgctcaagcg
      481 catggacggg gtccgaagca ctgcggagct gatcgcaacg gccgagacgg ccggatcacc
      541 agcaggtgat gccctggcaa tgctcaccga tctgcatcac agcggcctgg tgcaggacgc
      601 ctcggctgcc gacgacggtc caccgggctg gcaccgcgtg gcactggcca ccgaggcgac
      661 cagctggtcg gtgcacacca cctgcgcaac acggcgcgtg ctgcgccagc ggcgcgaggc
      721 tgcggtacgg gtggtcggat ccggccgggt ggcagtggcg ctggcaactt tgctggcagc
      781 ggccggcgta ggccaggtcg cggtggaagc caccggaacg gtcgcggccg cagacatcgg
      841 caccggctac cttccggacg atgtcggccg gacccgcgcg gccgcggcga cggatgccgt
      901 gcgcaggtgc gcgcccgagg cgaccggcac ccttcgccac cagccggacc tgattgtgtt
      961 gtgtgatgtg ccagtccccg agccggtcat ggtgaccgag ctgctcgcag ccgggatttc
     1021 gcacctgatc ggctacgccc acgaaggaac ggcagtggtc ggcccgctgg tgtgtccggg
     1081 tcgcagcagc tgcctgcatt gcgccgagct acaccgcgcg gactccgacc tggcctggcc
     1141 gaaactggcg gcccagctag ctgggcgggt ccagtccgcc ggattggcct gcacccagct
     1201 cgctgccgcg ttgaccgctg aacaggtact cgctgcgctg gccggatcac gagccggtct
     1261 gccggtcccg ccgacgtggg gtggcacgct cgaagtggac ccggtgcgcg gcacgttgca
     1321 tcgacatcct cggccagtcc acccacgctg tggctgcggt gcccgatgac gtctcaacac
     1381 cactacgccc ctcacaccac tactaagggg ggccgccccg ggagacaatc acccccgtga
     1441 ccgacatccc gcgcaaggcc gctgcgcgaa ctgcccggct ggcgagtctc ccgctcgggg
     1501 tagcgggccg ggtcgccgcc gggtggggca agcggttggc cgggcaggac gcgcgggacg
     1561 tcaacgccga gctcagtgct cgtaccgccg agcaactctt cgcggtgctc ggcgagctca
     1621 agggtggcgc gatgaagttc ggccaggcgc tgagtgtgtt cgaggcggcg atccccgacg
     1681 cgctcgccga accgtaccgg gaggc
//
LOCUS       DATADN010000252         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_22856, whole genome shotgun sequence.
ACCESSION   DATADN010000252 DATADN010000000
VERSION     DATADN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22856"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..873
                     /locus_tag="VHH53_03400"
                     /pseudo
     CDS             94..873
                     /locus_tag="VHH53_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            977..1447
                     /locus_tag="VHH53_03405"
     CDS             977..1447
                     /locus_tag="VHH53_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289245.1"
                     /translation="MTDDPDGIAELRAEIDAVERSALRHVDPGARATVLAGAIMVLLL
                     AITLPWVGDASGWDVLRGTANPRDQIGLLPRLFGAVALGFGVCLSVLALVTRRWGLVW
                     ATALGCTYGALDGIWAIWSRQTVDGPGPGIGMVLAVVAVLVLAVQWLRLALSRR"
     gene            complement(1462..>2136)
                     /locus_tag="VHH53_03410"
     CDS             complement(1462..>2136)
                     /locus_tag="VHH53_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEX2289246.1"
                     /translation="AFARRRGAHFAELDRQRVGARNRKEELVAQAEELAESTDWGTTA
                     ARFRNLMAEWKAAGRAPKEADDALWHRFRAAQERFFSRRSATFSARGSEFEHNLQRKE
                     ELIAAAERIDLTDIEAARAQLRGIQERWETVGKVPRERIKELEARLRAVEERIRSATN
                     AHWRHTAPEAQARVAQFRERLEQFEAQAAKARAAGDDRRATAAQAQADQWREWLAAAE
                     QAVTSR"
BASE COUNT          284 a          705 c          766 g          381 t
ORIGIN      
        1 gcccgcagca ccgcgtccga acccgactgt agcggcatgt gcagctggtg acagacattc
       61 ggggtctcgg ccatcgcctc gatgacatcg gaggtgaagt cctgcgggcg catcgacggc
      121 ctggaacggg tgcgcttcac ctcgccgcac ccgcgggact ttacctccga tgtcatccag
      181 gcgatggccg agaccccgaa tgtctgtcac cagctgcaca tgccgctaca gtcgggttcg
      241 gacgcggtgc tgcgggtgat gcgccgctcc taccgggcgc tgcgttacct tgcgatcgtc
      301 gatgaggtgc gggcggcgat gcccgatgca gcactgtcga ccgacatcat cgttggtttt
      361 cccggcgaaa ccgaagctga tttccaggcc accctggacg tggtgcgagc ggcgcgcttc
      421 agccaggcct tcacgttcca gtactcaccg cggccgggca cgccagctgc ggacatggct
      481 ggccaagtgc ccaaggcggt cgtgcaggac cgctacgagc ggctcgtcgc gttgcaggcg
      541 gagatctcct gggagctcaa ccgcggcctg gtcggtagcc agatggaggt gctggtctcc
      601 gagggcgagg gccgcaagga cgcggtgacc cggaggatga gcgggcgggc cagagacggg
      661 cggctggtgc acttcgatcc aggcgatgcc aaggtgcggc cgggggacgt ggtgctcacg
      721 gaaatcacct atggggcgcc gcaccacctg gtgtccgacc taccgttgcg agggcaccgg
      781 cagaccactg ccggcgaggc ttgggcagcc ggtcgccgcc ccacgacacc aggcgtggca
      841 ctcggcctac cctcgtttgg ccgaccaacg tgaccctctc cccggtatgg agacttatcg
      901 gggtcaccag gggttggatg ggccccgata aatctccatg tggggagttg gtcgggggct
      961 gggtggaggc ggtgatatga ccgacgatcc ggatggcatc gccgagctgc gggccgagat
     1021 cgacgccgtg gaacgctcgg cgctgcgcca tgtggaccct ggtgcccgcg cgaccgtgct
     1081 cgcaggtgcg atcatggtgc tgctgcttgc catcacgctg ccgtgggtcg gtgacgccag
     1141 cggctgggac gtgttgcgcg gtactgcgaa cccgcgtgac cagatcgggc tgctgccgcg
     1201 gctgttcggt gcagtagcgc tcgggttcgg ggtatgcctg tcagtgctgg cgctcgtcac
     1261 ccgtcgctgg ggcctggtct gggccactgc gctgggctgc acctacggtg cgctcgacgg
     1321 gatatgggcg atctggtccc ggcagaccgt ggacggcccg ggtccaggca tcgggatggt
     1381 gttggccgtc gtcgctgtgc tggtgctcgc cgtgcagtgg ttgcggctcg cgttgtcccg
     1441 ccggtaaccc ggccggccgg ttcaccggct ggtgactgcc tgctcggcgg cggccagcca
     1501 ttcccgccac tggtcagcct gggcctgcgc cgcggtggcg cggcggtcat ccccggcggc
     1561 acgggccttg gccgcttgcg cctcgaactg ctccagccgc tcccggaact gagccaccct
     1621 ggcctgagcc tccggggcgg tgtgtcgcca gtgtgcgttg gtggccgagc ggatccgctc
     1681 ctcgacggct cgcagccggg cttcgagttc cttgatccgc tcgcgcggga ccttgcccac
     1741 ggtctcccag cgctcctgga tgcctcgcag ctgcgctcgg gctgcctcga tatccgtgag
     1801 gtcgatccgc tctgcggcag cgatcagctc ctccttgcgc tgtaagttgt gctcgaattc
     1861 cgagccgcgc gcggagaagg tggcggagcg gcggctgaag aatcgctcct gggcagcgcg
     1921 aaaccggtgc caaagggcgt cgtctgcttc tttcggggcg cgcccagctg ctttccactc
     1981 cgccatcagg tttcggaagc gagccgcagt ggtgccccag tcggtggact ccgccagctc
     2041 ttcggcctgg gcgaccagtt cctccttacg attccgggcg ccgacacgtt gccggtcgag
     2101 ctcggcgaaa tgcgccccgc ggcgccgtgc gaaagc
//
LOCUS       DATADN010000253         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1385551, whole genome shotgun sequence.
ACCESSION   DATADN010000253 DATADN010000000
VERSION     DATADN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385551"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VHH53_03415"
     CDS             complement(<1..1527)
                     /locus_tag="VHH53_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX2289247.1"
                     /translation="MLYHHLDERRRYIVQRLAEVLRGATTGTPQALSERDAAAAMYSG
                     QLATLDGVEQGLCFGRLDLDGCDSDGEGSDDAGEERRYIGRLGLLDEERDYEPLLIDW
                     RAPAARPFYTATAACPEGVRRRRHIRTRSRTVLAVDDEVLDLDSAQQDPTPRTSLTSE
                     AALMAALSASRTGQMSDIVATIQVEQDRIIRAKPQGVLVVQGGPGTGKTAVALHRAAY
                     LLYTHRQQLAKRGVLVVGPNSTFLSYIGQVLPSLGETSVLLSTVAQLHPGLHACGIEP
                     AETAAVKGRAEMAKVVAAAVRDRQQVPAAPIELEVEAQPVRLDQQVCLPARTRARRSR
                     RPHNEAKRIFHREVVTALADQVASALGGGLLDRSDLADIRAELRASRELTAELDRLWP
                     TLSPEQLLTDLYADPQRLASATQRVLRPEQRDLLARRAPDPDTEPGQIWTPADVPLLD
                     EAAELLGSDGSAEAAREAAALREELQYAQGVLDILDLDEDLDPELLRATDLIDADRLA
                     D"
BASE COUNT          252 a          614 c          622 g          278 t
ORIGIN      
        1 gtcggccagc cggtcggcgt cgatcaggtc ggtggcgcgc aacagctcgg gatccaggtc
       61 ctcgtccagg tccaagatgt cgaggacgcc ctgcgcgtac tgcagctcct cgcgcagagc
      121 agctgcctcg cgggcggcct cggcactgcc gtcggagccg agcagctccg cggcttcgtc
      181 cagcagcggc acatcagcgg gtgtccaaat ctggccgggt tcggtgtccg ggtcaggcgc
      241 gcggcgggcc agcaggtcgc gctgctcggg acgcagcacc cgctgggtgg cgctggccag
      301 ccgttgcggg tcggcgtaga ggtcggtgag cagttgctcc ggcgacagcg tcggccatag
      361 ccgatccagc tcggcggtca gctcccggct ggctcgcagc tcggcgcgga tgtctgccag
      421 gtcactccgg tccagcagcc ccccacccag cgcgctggcg acctgatcag ccagtgcggt
      481 caccacctca cggtggaaga tccgcttggc ctcgttgtgc gggcgccggg agcgccgtgc
      541 ccgggtacga gccggcaggc acacctgctg gtccaaccgg acgggctggg cctcgacttc
      601 gagctcgatg ggcgccgcag gcacctgctg gcggtcccgt accgctgcgg ccaccacctt
      661 cgccatctcc gcgcgaccct tgaccgcggc cgtctcggcg ggctcaatgc cgcaggcgtg
      721 cagccctgga tgcaactggg ccacagtgga cagtagcacg ctggtttcgc ccagcgaggg
      781 cagtacctgc ccgatgtagc tcaggaaggt cgagttcggc ccgaccacca gcaccccccg
      841 cttggccagc tgctggcggt gcgtgtagag caggtaggcc gcccgatgca gcgcgaccgc
      901 ggtcttgccg gtgcccggcc cgccctgcac gaccagcact ccctgtggct tggcgcgaat
      961 gatccggtcc tgctcgacct ggatggtcgc cacgatgtcg gacatctggc cggtccggct
     1021 cgccgacagc gccgccatca gcgccgcctc actggtcagg ctggtacggg gcgtcgggtc
     1081 ctgctgggca ctgtcaagat cgagtacctc gtcgtcgacg gcgagcacgg tccggctgcg
     1141 ggtccggatg tgcctgcggc ggcgaacacc ctccgggcag gcggcggtcg cggtgtagaa
     1201 gggacgcgcc gcgggcgccc gccaatcgat gagcagcggc tcgtagtcac gctcctcgtc
     1261 gagcagtccg agccgcccga tatagcgccg ctcctcgccg gcatcgtcac tgccttcccc
     1321 gtcactgtcg cagccgtcga gatcgaggcg tccgaagcac aagccctgct ccaccccatc
     1381 gagcgtggcg agctggccgg agtacatcgc ggcggccgca tcgcgctcgc tgagcgcttg
     1441 gggcgttccg gtggtggcac ctcggaggac ctcggcgagg cgctgcacga tgtagcgtcg
     1501 gcgctcgtcg agatggtggt agagcatggt gaggtaggcc tgctcagcag ccagcgcttc
     1561 agagcttgtg gagggaaccg acacgcgatg catccacctt ctgcgatggg acggcgaacg
     1621 tggcactgaa accggtgggc cgctgccaac tttcaaggta gcaagacacg cactctcagg
     1681 tgtcctcgcc gtcccagcat ggtgatcgcg aacgagcggc ccgacgccgt cctcaatgga
     1741 gttcggcgtc gagccgggta gcgtcg
//
LOCUS       DATADN010000254         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1086565, whole genome shotgun sequence.
ACCESSION   DATADN010000254 DATADN010000000
VERSION     DATADN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086565"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..940)
                     /locus_tag="VHH53_03420"
     CDS             complement(371..940)
                     /locus_tag="VHH53_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEX2289248.1"
                     /translation="MTIMTLSKFACAMNEFDRRVRAIRPDQWRNPTPCTEWDVHALVD
                     HLVTEQLWVPLLLDGATVEDVGDRFDGDQLGDDPVAAWETAAAAAREAFAAPGALRRP
                     VELSYGRRPADGYCREMTMDLTVHAWDLARGIGADERLDEELVRDVLNFIEPQVEQLI
                     GTGLFAAPVAVGEDVDAQTRLLALLGRRA"
     gene            complement(995..1351)
                     /locus_tag="VHH53_03425"
                     /pseudo
     CDS             complement(995..1351)
                     /locus_tag="VHH53_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
     gene            complement(1376..>2127)
                     /locus_tag="VHH53_03430"
     CDS             complement(1376..>2127)
                     /locus_tag="VHH53_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX2289249.1"
                     /translation="ATPVAVTNCLNFGAPTDPGVMWQFQQAARGLADGCAALGIPVTG
                     GNVSFYNQTGDIGILPTPVVGVLGVIDDVRRRIRSTLGDPGDEVWLLGDSRDEFGGSE
                     WAHEVHGHLGGRPPAVDLAREQAIGEVLAAASRDGLISGAHDLSDGGLAQALVEAVLL
                     SGHGARIELPAQLDPFVTLFTESAGRVLLGVSGSHARRVAQLCTAYNVPVTPLGEVHA
                     EPMLDIAGVATFSLDELRAAWEATMPALFAP"
BASE COUNT          339 a          784 c          706 g          298 t
ORIGIN      
        1 agccgccgca cagcccgcag cggtccgcca ggcaccacag tccattccgg tgcctccggc
       61 cacggaggac ctcgctgaga acgtcccgac cgccgagcgg cccgccccgc aggcaccagc
      121 cgccgcgcca cccccggtca ccaagcgcga agccgccgtt ccccgggcca gaaagcagcc
      181 gggtgcgcca gcccagcgac gtaacgccga cgttccccgg acgtcaccgt caactcggga
      241 ctcacgctcc agccagcggc cacgctctga tcgggactct gctcggggag agcggtccgg
      301 gctgggttcg acgttgaacc ggacgaggga gtccgttgaa tcggggttgg gctcgcgttg
      361 agcccagcgc tcatgcccgc cggccgagca gggcaagcag tcgggtctgg gcgtcgacgt
      421 cttcgcccac tgcgacgggc gcggcgaaca atcccgtgcc gatcagctgc tcgacctgcg
      481 gctcgatgaa attcagtacg tcacgcacca gctcctcgtc gagacgctcg tcagctccga
      541 tcccacgggc caaatcccac gcatgcacgg tcaggtccat ggtcatctct cggcaatatc
      601 catcagcggg tcggcgcccg taggacagct caaccggtcg ccgcagagcg ccaggggctg
      661 cgaacgcctc ccgcgccgcg gccgcggcag tctcccaggc cgccaccggg tcgtccccga
      721 gctgatcgcc atcgaagcga tcgccgacgt cctcgacagt ggcaccgtcg agcagcagcg
      781 gcacccacag ctgctcggtg accaggtgat cgaccagagc gtgcacgtcc cactccgtgc
      841 acggggtcgg gttgcgccac tggtctggcc ggatggcccg cacccggcgg tcgaattcgt
      901 tcattgcgca cgcgaacttc gacagcgtca tgatcgtcat ggtgcgcgac gtgctggccc
      961 cgtgcgcgtc cagccccgcc ccgcctgccg cattcagcgg taaccatgcg gagagattcg
     1021 ctcgggcgcc ggatgcagcg acccgcgcgt cggcgatcgc cgcggtccag accgctcggc
     1081 cggtcgcgag ctcaagccaa gtcctcgggt cggtctccac cacgttgggc ggggtacccc
     1141 gggtgtgtct cggtccgggg acgcactgca ccgcggcgaa cggcggcacc cgcacttcca
     1201 ccgtgcgccc cggagcgatc tgcgccaggg tgcgcaggct gagccggaca gcctgcgcca
     1261 gctcggcgcg cccaggctgc ggctcatttc cttccagcca agcctcgacc gctgcgagcg
     1321 ccccccgcaa ctcatccgga tcgactctca tcactctccc cgcgtaacgc ggcgctcagg
     1381 gggcgaacag tgcgggcatg gtggcctccc aggcggctcg tagctcgtcc aggctgaatg
     1441 tggcgacacc agcaatgtcc agcatgggct cggcgtggac ctcgccgagt ggggtcaccg
     1501 gcacgttgta ggccgtgcac agctgcgcga cacggcgagc atgtgagccg gacacgccga
     1561 gcaggacgcg gccagcggac tcggtgaata gggtgacgaa cggatcgagc tgggcaggca
     1621 gctcaatccg cgcaccgtgg ccgctgagca aaaccgcttc gaccagcgcc tgcgcgagac
     1681 caccgtcgga caggtcgtgc gccccgctga tgaggccgtc gcgggaagct gcggcgagca
     1741 cctcgccgat ggcctgctcg cgggccaggt ccacggcggg tgggcgaccg ccgagatgcc
     1801 cgtgcacttc gtgtgcccat tcgctaccgc cgaactcgtc gcggctatcg ccgagcagcc
     1861 agacctcgtc accgggatcg cccaaagtgc tgcggatccg gcggcgcaca tcgtcgatca
     1921 cgccgagtac acccaccacc ggggtgggca ggatcccgat gtccccggtc tggttgtaga
     1981 aactcacgtt cccgccggtg accgggatac cgagtgctgc gcagccgtcg gccagaccgc
     2041 gggctgcctg ctggaactgc cacatcacgc ccggatcggt gggcgcaccg aaattgaggc
     2101 agttcgtcac cgccaccggg gtggcgc
//
LOCUS       DATADN010000255         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1087114, whole genome shotgun sequence.
ACCESSION   DATADN010000255 DATADN010000000
VERSION     DATADN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087114"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VHH53_03435"
     CDS             complement(<1..837)
                     /locus_tag="VHH53_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX2289250.1"
                     /translation="MSASAANSSGPATGAAAPRDLLAGLNPQQRAAVQHAGSPLLIVA
                     GAGSGKTRVLAHRIAYLLDQRGVAPGQVLAITFTNKAAAEMRERVTALVGKRAAVMWV
                     STFHTMCVRILRREARLLSGNLSGLTSNFSIYDADDTRRLITMVSRDLDLDPKRYPAR
                     TLAAKISNLKNELANPDSAAATASDDLDRRVAEVFAAYQRRLASSNALDFDDLIMRTV
                     ELLQTFPDVAEHYRRRFHHVLVDEYQDTNHAQYVLVRELVAPPVEGERPAELCVVGDA
                     DQS"
     gene            1006..>1543
                     /locus_tag="VHH53_03440"
     CDS             1006..>1543
                     /locus_tag="VHH53_03440"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2289251.1"
                     /translation="MDPVSWLEHVSKASPALLLVVVVLVMFVDGLALIGAMLPGDLLL
                     LVPAAAVGLDGAPIVVAGAVIGTVLGYSVSYAVGCRNGAAIRHSWLGQRVRPERWKQA
                     EQLLRGPAGRTLALVQFMPLLNYLIPLLAGTLRVPPKRFIRLTATGALMYSTFYVVLG
                     VLAGGTGQAIYDSTGRLVA"
BASE COUNT          243 a          487 c          544 g          269 t
ORIGIN      
        1 ggactggtcg gcgtcaccca ccacgcacag ctccgcgggc ctttctccct cgaccggcgg
       61 tgcgaccagt tcgcggacca gcacgtattg cgcatggttg gtgtcctgat actcgtcgac
      121 caggacatgg tgaaaccggc gccggtagtg ctcggcaaca tcagggaagg tctgcagcaa
      181 ctcgacggtg cgcatgatca gatcgtcgaa gtccagcgcg ttcgacgaag ccaaccgccg
      241 ctggtaggca gcgaacacct ccgccacccg gcgatcgagg tcatcggaag cggtggcggc
      301 ggcactatcg gggttagcca gttcgttctt caggttcgag atcttcgctg ccagggtccg
      361 cgccgggtag cgcttggggt ccaggtccag atcacgggag accatcgtga tcaggcgacg
      421 ggtgtcgtcg gcgtcgtaga tggagaagtt cgacgtcagc ccggaaaggt ttcccgagag
      481 gagccgagct tcccggcgca ggatccgcac gcacatcgtg tggaacgtcg acacccacat
      541 caccgcggca cgcttgccga ccagcgcggt gacccgctcc ctcatctcgg cagcggcctt
      601 gttcgtgaag gtgatcgcca gcacctgtcc gggcgccaca ccgcgctggt cgagcaggta
      661 ggcgatgcgg tgggcgagca ctcgggtctt gccggacccg gctcccgcga cgatcagcag
      721 cggggaaccg gcatgctgca ccgccgcgcg ctgctgcggg ttgagtccgg cgagcagatc
      781 ccgtggagcg gctgcgccgg tagcggggcc agaagagttg gcagcggagg cactcatcgt
      841 ggcgaccacg ttaccggcaa gcaccgaccg tccgggtatt gcttggtatc gctgagagtc
      901 cgctgaggcg gagtccaccg atacgaccgg tgtggcggat aatcctctca gaaggcagag
      961 tttcaccgag gccaccccgg cagagccaga ccggcaggag gtgccgtgga tcccgtcagc
     1021 tggcttgagc acgtgtcgaa ggcttctcct gccctgctgc tggtcgtggt ggtgctggtg
     1081 atgttcgtgg acggcctggc cctgatcggt gcgatgcttc cgggtgacct gctgctgctg
     1141 gtcccggcgg ccgcggtggg cttggacggc gcgccgatcg tggtggcggg tgccgtgatc
     1201 ggaaccgtgc tgggctacag cgtcagctac gcggtgggtt gccgcaacgg tgccgccatc
     1261 cggcacagtt ggctgggcca gcgggtccgg cccgaacggt ggaaacaggc agagcagctg
     1321 ctgcgtggcc cggcaggtcg caccctggcg ctggttcagt tcatgccact gctcaactac
     1381 ctgatcccgt tgctggcggg caccttgaga gtgccgccga agcgcttcat ccggctcacc
     1441 gcaaccggcg cactgatgta ctcgaccttc tacgttgtcc tgggggtgct ggccggcggg
     1501 accggtcagg cgatctacga ctccacaggc cggctggtgg cgc
//
LOCUS       DATADN010000256         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1087331, whole genome shotgun sequence.
ACCESSION   DATADN010000256 DATADN010000000
VERSION     DATADN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087331"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VHH53_03445"
     CDS             <1..1506
                     /locus_tag="VHH53_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX2289252.1"
                     /translation="PPPPDQLPVVAAPWSTWTPSGWLGVELGQAGAVPTALSDTDLIE
                     GVIAFDRMASWAGARQTALLAEFAHRRPDDHPLAVNSDIPSRASEFAADEIALALRLS
                     RTTAANRLVMAETLAAELPCTLAAWEAGVIDALKARAITETSYLLPPEKRGTLEARVL
                     PRAGTQTIAQLRAALARAVLALDPQGAAERHQQRHQHRRVVVCPDEEGMASLWALLSA
                     PDATAAYQRLCDLARGLGGEDPRGMDARRADLLVELLTGRRCAATGFCVDPDCGCAGD
                     PTADLASADPAPVDTDPSSTECVAAESGLQVTSAAGPDRGGHRCAASQGQGAGPGKPL
                     VSVIVPITMLLGLDDQPAELVGYGPIPADLAREIAAEGTWRRLLTDPVSGALLDYGRT
                     TYTPPAGLAGFVRARDVYCRNPICRQRAATADLDHTIAWDDGGTTCQHNLWAGCRHDH
                     RLKTFAPGWTVDQYPDDRITFTTPTGHIYTSHPHDYRPDFHPPPGDDPPPF"
BASE COUNT          241 a          567 c          549 g          268 t
ORIGIN      
        1 ccgccaccac cggatcagtt gccggtggtg gcggcaccgt ggtcgacgtg gacgccgtcg
       61 ggatggctgg gtgtcgaatt gggccaagcc ggcgccgtgc ctacggcgct gtcggatacg
      121 gatcttattg agggtgtcat cgcgttcgac cggatggcgt cgtgggccgg ggcgcgccag
      181 accgcgttgc ttgcggagtt cgcccatcgc cgcccagatg atcacccgtt agcggtcaat
      241 tcggatatcc cgtcgcgggc cagtgagttc gctgcggatg agatcgccct agcgttgcgg
      301 ttgtcgcgga ctactgcggc taaccggttg gtgatggctg agactttggc cgcggaactg
      361 ccctgcaccc tggctgcctg ggaggccgga gtgatcgacg cgctcaaggc gcgggcgatc
      421 accgagacca gttacctgct cccaccggag aagcggggca cgctggaggc gcgggtgttg
      481 ccccgcgcgg ggacgcagac gatcgcccag ttacgggccg cgcttgctcg ggcggtgctg
      541 gcgctggacc ctcagggggc agccgagcgt caccagcaac gtcaccagca ccgacgggta
      601 gtggtctgtc ccgatgagga gggcatggcg tcgctgtggg cattgctttc cgccccggat
      661 gccaccgccg cctaccagcg gctgtgtgac ctggcccgtg ggctaggtgg tgaggatccg
      721 cgagggatgg acgcccggcg tgctgacctg cttgtggagt tgcttaccgg gcggcgctgt
      781 gcggccaccg gcttctgcgt cgaccctgac tgcggctgtg ccggcgatcc caccgccgac
      841 ctcgcgtctg ccgaccccgc gccagttgac accgatccct ccagcaccga gtgcgtagcc
      901 gctgagagtg gcctgcaggt caccagtgcc gctgggccgg accggggtgg ccaccgctgc
      961 gccgcatctc agggtcaggg cgctgggccg ggcaagccgc tggtgtcggt gatcgtgccg
     1021 atcaccatgc tgctcggcct ggatgatcag cccgcagagc tggtcggcta cggacccatc
     1081 cctgcggatc tggctcgcga gatcgctgcc gagggaacct ggcgacggtt gttgaccgac
     1141 ccggtctcag gcgccctgct cgactacggc cgtaccacct acaccccgcc ggctgggctg
     1201 gccggcttcg tccgcgcccg cgatgtgtac tgccgtaacc cgatctgtcg gcaacgtgca
     1261 gccaccgccg atctggatca caccatcgcc tgggacgacg gtggcactac ctgccagcac
     1321 aacctgtggg ccggctgccg ccacgatcac cgactcaaga ccttcgctcc cggctggacc
     1381 gtagaccaat accccgacga ccggatcacc ttcaccaccc ccaccggaca catctatacc
     1441 agccaccccc acgactaccg acccgacttc caccccccgc ccggcgacga tccgccgccc
     1501 ttctagatcc acatcatggg ctggaacaac ccgccggtgc ggtgggcgga cctggagcgg
     1561 gcactgtccg gacgtgccgc cgattccaga tcagggcact gcgacgagga gcatcccggt
     1621 gacgg
//
LOCUS       DATADN010000257         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_333995, whole genome shotgun sequence.
ACCESSION   DATADN010000257 DATADN010000000
VERSION     DATADN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333995"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VHH53_03450"
     CDS             <1..1545
                     /locus_tag="VHH53_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005445323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX2289253.1"
                     /translation="LRTVIDEVSGQLTEARVKAEVLGRPKHYYSIYQKMIVRGRDFDD
                     IHDLVGVRIMVEDVRDCYAAVGVVHSVWQPMPGRFKDYIAQPRFGVYQSLHTTVIGPD
                     GQPLEVQIRTRDMHRTAEYGIAAHWRYKETRGTHNGAVDVDEMAWMRQLLDWQREAAD
                     PGEFLESLRYDLAAREIFVFTPKGDVITLPTGSTPVDFAYAVHTEVGNRCIGARVNGR
                     LVALERQLENGEVVEIFTSKAEGAGPSWDWLSFVASPRARAKIRQWFAKERREEAIDT
                     GKDAIAKELRRVGLPVQRLVSAESMVAVSRELHYPDVSALYAAVGEHHASSRQVVQRL
                     VALLGGEEGAEEVLADRATPSTVQRRRETGDSGVRVRGAGDVYAKLARCCTPVPGDEI
                     LGFVTRGGGVSVHRTDCTNADSLREQPERLVDVEWAPSSSSVFLVGIQVEALDRHRLL
                     SDVTKVLADGRVNILSASVTTTRDRVAVSRFSFEMGDPKHLGHVLRAVRNIEGVYDVY
                     RVTSSN"
     gene            complement(1571..>1766)
                     /locus_tag="VHH53_03455"
     CDS             complement(1571..>1766)
                     /locus_tag="VHH53_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2289254.1"
                     /translation="LIYGQTQLPPDYTVFATISPEGLKVIDEIARAGDDGSLEPSPGG
                     GKPNAPVTITSVDVAPDIAP"
BASE COUNT          281 a          559 c          623 g          303 t
ORIGIN      
        1 ctgcgcaccg tgatcgacga ggtgtccggc cagctcaccg aggcgcgggt gaaggccgag
       61 gtgctcgggc ggcccaagca ctactactcg atctaccaga agatgatcgt gcgcggtcgc
      121 gatttcgacg atatccacga cctggtcggg gtacggatca tggtcgaaga cgtgcgcgac
      181 tgctatgccg cggtcggcgt ggtgcactct gtgtggcagc cgatgcccgg gcggttcaag
      241 gactacatcg cccagccccg cttcggggtc taccagtcgc tgcacaccac ggtgatcggg
      301 ccggacgggc agccgctgga ggtgcagatc cgcacccgcg atatgcaccg aaccgcagag
      361 tacggcatcg ccgcgcactg gcggtacaag gagacccgcg gcacccacaa cggagctgtg
      421 gacgtcgatg aaatggcctg gatgcgccag ttgctggact ggcagcgtga ggccgccgac
      481 cccggtgagt tccttgagtc gctgcgttat gacctggccg cccgtgagat cttcgtcttc
      541 acacccaagg gcgacgtcat cacgctgccc acaggttcca cgccggtcga cttcgcctac
      601 gcggtgcaca ccgaggtcgg taatcgctgc atcggtgcgc gggtcaacgg ccggctggtc
      661 gcgttggagc ggcagttgga gaacggcgag gtcgtcgaaa tcttcacctc gaaggccgag
      721 ggtgccgggc ccagctggga ctggttgtcg ttcgtcgcct caccacgggc ccgggccaag
      781 atcaggcagt ggttcgccaa ggagcgccgc gaggaggcga tcgacaccgg caaggacgcc
      841 atcgccaagg agctgcgccg ggttggtcta ccggtgcagc ggttggtgtc tgccgagtcg
      901 atggtcgcgg tgtctcgaga gctgcactat cccgacgtca gcgccctgta tgccgcggtc
      961 ggggagcatc acgcctcgtc tcgtcaagtg gtgcagcgcc tggttgcgct gctggggggt
     1021 gaggagggcg ccgaggaggt gttggccgac cgggccaccc cctccacggt ccagcgccgc
     1081 cgcgagaccg gcgactccgg cgtgcgagtg cggggtgccg gggacgtcta cgccaagctc
     1141 gcccgctgct gcacaccggt ccccggtgac gagatcctgg ggtttgtcac caggggtggc
     1201 ggggtcagtg tgcaccgcac cgactgcacc aatgccgact cgctgcggga gcagcccgag
     1261 cggctggttg atgtggagtg ggcgccgtct tcctcgtcgg ttttcctggt cggtatccag
     1321 gtcgaggcgc tggaccggca ccggctgcta tctgacgtca ccaaggtgct cgctgacggg
     1381 cgggtgaaca tcctgtcggc ctctgtcacc accacccggg accgggtggc ggtgagccgg
     1441 ttctccttcg agatgggtga ccccaaacac ctcgggcacg tgctccgggc agttcgcaat
     1501 atcgagggcg tctacgacgt ctaccgagtc acgtcatcga attgagcgcc ggcccagcga
     1561 cccgaccctg ttacggcgcg atgtccggcg cgacgtcgac ggacgtgatc gtgaccggcg
     1621 cattgggttt accgccacca ggggatggtt ccagcgaccc gtcgtcacca gcccgcgcta
     1681 tctcatcgat caccttgagg ccctcggggg agatcgtggc gaagacggtg tagtcgggcg
     1741 ggagctgggt ctgaccgtag atcaag
//
LOCUS       DATADN010000258         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_937226, whole genome shotgun sequence.
ACCESSION   DATADN010000258 DATADN010000000
VERSION     DATADN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937226"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VHH53_03460"
     CDS             complement(<1..790)
                     /locus_tag="VHH53_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2289255.1"
                     /translation="MSSAISGAVDLSALKARADTAGRPQPPGADPSHGGTARVVDVTE
                     QSFQTDVVDRSMQVPVVVDLWATWCGPCKQLSPVLERLAEAAGGAWVLAKVDVDANPR
                     IAQLFGVQSIPTVIAIAAGQPIDAFAGVQPEPQVRQWITSILDALRDQLPGIRAAEHG
                     APGEGTAMPVADEPRFTAAEAAVAAGDYAAAEAAYQQILDAEPANVEARAALVQVRFL
                     ARAESTDPAAVARADAAPQDVDAQLAAADVEVAGQQPERAFERLV"
     gene            complement(880..>1614)
                     /locus_tag="VHH53_03465"
     CDS             complement(880..>1614)
                     /locus_tag="VHH53_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2289256.1"
                     /translation="TAGVSLTAQQPYNNVVRTAIEALAGVLGGTNSMHTNALDETLAL
                     PSERAAEIALRTQQVNMEESGVTNVADPLGGSWYVEALTDRIEAEAERIFGRIKDMAA
                     AAEPSGRHPIGEMTSGILRGIEDGWFTDEIADAAFAYQQALEKGDKKIVGVNTLTESV
                     TDELEILRVSHEVECGQNRVLQQRSSQRDGAAVDRALAAMVAVANGEENLIEPMLEAA
                     RAEATLGEICGALKAQWGEYREPARF"
BASE COUNT          227 a          577 c          537 g          273 t
ORIGIN      
        1 cgaccaaccg ctcgaacgcc cgctccggct gctggccggc cacctcgaca tcggccgcgg
       61 cgagctgcgc gtccacgtcc tgtggcgcgg cgtcggcccg ggcgaccgca gcggggtcgg
      121 ttgattcggc gcgggccaga aagcgcacct gaaccagcgc ggccctggcc tccacgttgg
      181 ccggctcggc atccaggatc tgctggtagg ccgcctcggc agcggcgtag tcaccagccg
      241 ccacggcggc ttcggcggcg gtgaagcggg gctcgtcggc taccggcatt gcggtgccct
      301 cacctggcgc accgtgctcg gcggcccgaa tcccgggcag ctggtcgcgc agcgcgtcga
      361 ggatcgaggt gatccactgc cgcacctgcg gctcgggctg gacaccggcg aaggcgtcga
      421 tcggctgacc ggcggcgatg gcgatgacgg tagggatcga ctggacgccg aagagctggg
      481 caatccgcgg attggcatcg acgtcgacct tggccagcac ccaggctcca ccggctgcct
      541 cagcgagccg ttcaagcacc ggggacagct gcttgcacgg gccacaccac gtcgcccata
      601 gatcaaccac cacaggcacc tgcatggagc ggtcgacgac atcggtctgg aaactctgct
      661 cagtgacgtc gacgacccgg gcagtgcctc cgtggctcgg gtcggcaccc ggaggctgtg
      721 gccggcccgc cgtatcggcc cgcgccttga gcgcggacag gtcgacggca ccggagatgg
      781 cgctggacat cgctgcggtc tgttgcgggt taggcctgct cacaccgtct atcctggcat
      841 ggtggccacg cgagctttgg ctccgggcac atcattgggt cagaatctgg ccggctcgcg
      901 gtactcaccc cactgcgcct tgagcgcccc gcagatctcg ccgagggttg cttcagccct
      961 cgccgcctcg agcatcggct cgatgaggtt ttcttcgccg ttagctaccg cgaccatcgc
     1021 cgcaagagcg cggtcgacgg cggccccgtc gcgctgcgag ctgcgctgtt gcagtacccg
     1081 gttctgcccg cactccacct cgtggctgac ccggaggatc tccagctcgt cggtgaccga
     1141 ttcggtgagc gtgttcaccc cgacgatctt cttatcgccc ttctccaatg cctgctggta
     1201 agcaaatgcc gcgtcggcga tctcgtcggt gaaccagccg tcctcgatgc cccgcaggat
     1261 gcctgacgtc atctcaccga tcgggtggcg gcctgatggc tccgcggccg ccgccatgtc
     1321 cttgatccgg ccgaagatcc gctcagcctc ggcctcgatc cggtcggtga gcgcctcgac
     1381 gtaccaggag ccgcccaacg ggtcggccac gttggtcacc ccggactcct ccatgttcac
     1441 ctgttgagtg cgcagcgcaa tctcggcggc ccgctcgctg ggcagcgcca gcgtctcgtc
     1501 cagagcattg gtgtgcatcg agttcgtgcc gcccagcact cccgcgagcg cctcgatcgc
     1561 ggtgcgcaca acgttgttgt agggctgctg cgcggtgagc gagacccccg cggt
//
LOCUS       DATADN010000259         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_937393, whole genome shotgun sequence.
ACCESSION   DATADN010000259 DATADN010000000
VERSION     DATADN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937393"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1572)
                     /locus_tag="VHH53_03470"
     CDS             complement(<1..>1572)
                     /locus_tag="VHH53_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2289257.1"
                     /translation="IGRVVDDSIVVIENIKRHISYGGPRGAAIVTAVGEVAGAITAST
                     VTTVAVFAPIGLVGAQVGELFRPFAVTVTAALLASLLVSLTVGPVLASAVLRSSTHPQ
                     PRPADTNGDDADGSPATRTVLQRGYLPVLQVALHRPVLSLVVAAAILLGTMTLSSQLR
                     TNFLGDAGGDTLSVSQELAPGTGQPQADAAAKRVEGVLAETRGITSYQVTVGSPADAS
                     GFGPASSATQFSATLAAGANASAVSDDLRARLLALGEPEEVGTLTVQTGQGGNDQLLV
                     VVRAEDREVLAQAADQVQKAVASIPGAADVRNNLAAAQPIVQVVVDRWKAARDGLTEA
                     QIGQEVSTALSGTTVGTLTIDGVEQSVRVRTGTAPGDLADLRDLPLPTANGTVELSDV
                     ATVKQSTTPPSISHTDGSRSAQITARPAADDLGAVTAALQAKLDELDLPAGATAEIGG
                     VSADQSDAFAELTLALLVAIAVVYMVMVVTFRSLLQPMVLLVAIPFAATGALGLLLLT
                     NTPLDVPALIGMLML"
BASE COUNT          253 a          548 c          543 g          228 t
ORIGIN      
        1 cgagcatcag catcccgatc agtgcgggga catcaagcgg cgtattggtg agcagcaaca
       61 gaccgagcgc accggtggcc gcgaacggga tcgcgacaag cagcaccatg ggctgcagca
      121 ggctgcggaa cgtcaccacc atgaccatgt agaccacggc gatcgcgacc agcagggcca
      181 gggtgagttc cgcgaacgcg tcactctggt cggcgctgac accaccgatc tcggcagtgg
      241 cgccggccgg gagatccagc tcgtcgagct tggcctggag cgcggcggtg accgcaccaa
      301 ggtcatcggc ggccggccgc gcggtgatct gggcgctgcg ggaaccgtcg gtgtggctga
      361 tgctcggtgg ggtggtcgac tgcttcaccg tcgcgacgtc ggacagctcc acggtgccat
      421 tggcggtcgg cagcggcagg tctcgcaggt ccgcgagatc cccgggagcc gtcccggtgc
      481 gtacccgcac gctttgttcc accccgtcaa tggtcagggt gccgacagtg gtgccgctca
      541 gcgccgtcga cacctcctgc ccgatctgcg cctcggtgag cccgtcccgt gcggccttcc
      601 accggtcaac cacgacctgg acgatgggct gtgcagcagc caggttgttg cgcacatcgg
      661 cggcacccgg gatggacgcg acggcctttt gcacctgatc ggcggcctgc gcgaggacct
      721 cacgatcctc ggcccgcacc acgacaagca actgatcgtt gccgccctgg ccggtctgca
      781 cggtcagcgt gccgacctcc tcgggctcac ctaacgcgag gaggcgggcg cgcaggtcat
      841 cggatactgc ggacgcgttc gcaccggcgg cgagcgtggc cgagaactgg gtggcgctgc
      901 tggctggacc gaagccagat gcgtcggccg gcgacccgac ggtcacctgg taactcgtta
      961 tcccgcgggt ctcagccagc acaccctcga cccgctttgc cgcggcgtcg gcctgcggtt
     1021 gcccggtgcc gggggccagc tcctggctca ccgacaatgt gtcgccgccc gcgtcgccga
     1081 gaaagttcgt gcgcagctgg gaggacagcg tcatggtgcc cagcagaatc gccgccgcga
     1141 ccactagcga cagcaccggt cggtgcagcg ccacctgaag caccggcagg tagccccgct
     1201 gcagcacggt gcgcgtagct ggtgacccgt cggcatcgtc accgttggtg tcagccgggc
     1261 gcggctgggg atgcgttgaa ctccgcagca ctgccgaggc cagcaccggc cccacggtca
     1321 gcgacaccag cagcgacgcg agcagtgccg cggtcacggt gaccgcgaaa ggccggaaca
     1381 gctcgccaac ctgcgcaccg accaggccga ttggtgcgaa cacggccacc gtcgtcaccg
     1441 tcgaggcggt gatggcgccg gccacctcgc cgacagccgt gacgatcgcg gcaccccgcg
     1501 ggccgccgta gctgatatgg cgcttgatgt tctcgatgac aacgatcgag tcgtcgacga
     1561 cccggccgat cg
//
LOCUS       DATADN010000260         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_23901, whole genome shotgun sequence.
ACCESSION   DATADN010000260 DATADN010000000
VERSION     DATADN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23901"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VHH53_03475"
     CDS             complement(<1..1147)
                     /locus_tag="VHH53_03475"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX2289258.1"
                     /translation="MTQTLMLRDVTEVRRDGDDVVLSVRALRAMELPGTPGYLTGEAP
                     AEQGIETGLPNLPDLKLPPLEAEPYVLRIGFAAPDTVRLTVAPEGARVLSDDGTWLAI
                     VTEPRPRPVVAQVEENADTVVVASPRMRVQVWRHPFALLVEDAESGTVLLRTAERLRQ
                     VGGFPMSPTVLVDGRGTTLHLELGVDEDVLGFGEQFGRLVKNGQRLVLRVEDALGTGT
                     GLAYKPVPVWHSSAGYTGLLNTGAIVTADVGHSRPSVLGLTVADEAVDLYVVAGRSAK
                     ERLGSYTALTGRGPVPPLWAFGYWMGRCRYHSRTEMEEVGRRMRQHQVPCDVLHLDPD
                     WLVVDRLNCDFIWNTDRFGDRREFVAALEKLGCRISVWELPYLDPASP"
     gene            complement(1156..1971)
                     /locus_tag="VHH53_03480"
     CDS             complement(1156..1971)
                     /locus_tag="VHH53_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2289259.1"
                     /translation="MGYQLGRRTLLIVYAVLVIVPLFVVFSGSLKTTQELFDSPFGLP
                     TSPETGNYGRVLGEQNLGRAFLNSTIVTLISVTLTLFIASLAAYGLARIPGWKGLAVY
                     GFIVLGMAVPAQANMIPQYVLFDVLGLLNSLFGLALINIVITLPIAVFILTGFMKTLP
                     REIYEAAALDGSGPWRTYRKIAMPLSVPSLAATAIFLFVIHWNDLLYPLLFIQEPDKK
                     TLPVALLDFTGEFLTDYPLLFTGVVIASTPIVIAYVLLQRYFIAGMTAGSVKG"
BASE COUNT          370 a          706 c          737 g          294 t
ORIGIN      
        1 gcggcgaggc cggatcgagg tagggcagct cccacaccga gatccggcag ccgagcttct
       61 ccagcgcggc gacgaactcg cgccggtcgc cgaaccggtc ggtgttccag atgaagtcgc
      121 agttgagccg atcaacgacg agccagtccg ggtccaggtg gagcacgtcg cagggcacct
      181 ggtgctggcg catccggcgt ccgacctcct ccatctcggt gcgtgagtgg tagcggcagc
      241 ggcccatcca gtagccgaac gcccacagcg gcggcaccgg gccgcgcccg gtgagcgcgg
      301 tgtacgaacc cagccgctcc tttgccgacc ggccggcgac gacatacagg tccaccgcct
      361 cgtcggccac ggtgagcccc agtacgctgg gccggctgtg accgacgtcg gcggtgacga
      421 tggcgccggt gttcagcaag ccggtgtagc cggcgctgga gtgccacacc gggaccggct
      481 tgtaggccag cccggtgccg gtgccgagcg catcctcgac gcggagcacc agccgctggc
      541 cgttcttgac caagcggccg aactgctccc cgaagccgag gacatcctcg tcaaccccga
      601 gctcgaggtg cagcgtggtc ccccgcccgt cgaccagcac tgtgggcgac atcgggaacc
      661 cgccgacctg ccgcaaccgc tcggcggttc gcagcagcac cgtgccgctc tcggcgtcct
      721 ccacgagcag cgcaaagggg tgccgccaga cctggacccg catccggggg ctggcgacca
      781 ccaccgtgtc ggcattctcc tcgacctggg ccacaacagg cctgggccgg ggctcggtga
      841 cgatggcgag ccaggtgcca tcgtcgctga gcaccctcgc gccctccggg gcgacggtga
      901 gccgcaccgt gtcgggcgcg gcgaagccga tccgcagcac gtacggctcg gcctccagcg
      961 gcggcagctt caggtccggc aggttcggca ggccggtctc gatgccctgc tcggcgggcg
     1021 cttccccggt gagataaccg ggcgtgccgg gcagctccat tgcgcgcagc gcgcgaaccg
     1081 acaggaccac atcgtcaccg tcgcggcgca cctcggtgac atcgcggagc atcagcgtct
     1141 gggtcacgac cccggtcatc ccttgacgga tccggcggtc atgccggcga tgaagtaccg
     1201 ctgcagaagc acgtaggcga tgacgatcgg ggtggaggcg atcacgacgc cagtgaacag
     1261 cagggggtag tcggtgagga actcaccggt gaagtccagc agcgcgaccg gcagcgtctt
     1321 cttgtccggc tcctggatga acagcagcgg gtagagcagg tcgttccagt ggatgacgaa
     1381 caggaagatc gcagtcgccg ccaacgacgg caccgacaac ggcatggcga tcttgcggta
     1441 ggtgcgccag gggcccgagc cgtccagggc cgcggcctcg tagatctccc gtggcagcgt
     1501 cttcatgaac ccggtgagga tgaagaccgc gatcggcagg gtgatgacga tgttgatgag
     1561 ggccaaaccg aacaggctgt tcagcaggcc aagcacgtcg aacagcacgt actgcgggat
     1621 catgttggcc tgggccggga cggccatccc gagcacgatg aacccgtaca cggccagccc
     1681 cttccagccg gggatccggg ccaggccgta ggccgcgagg ctggcgatga acagggtcag
     1741 cgtcaccgag atgagggtga cgatcgtgct gttgaggaac gcgcggccca ggttctgttc
     1801 cccaagcacc cgtccgtagt tgcccgtctc gggtgaggtc ggcaggccga acggggagtc
     1861 gaacagctcc tgggtggtct tcaggctgcc ggagaagacc acgaacagcg gcacgatgac
     1921 cagcaccgcg tacacgatca gcagggtccg gcggccgagc tggtatccca tctgggtcct
     1981 cccctcaggc cacgccctgg gtgagccgca ccgcacggcg ctgcagccag gtgacggcaa
     2041 ggatcacaat catgaacagg atggactcgg cggcggcgta gccgaactga gagttcgcga
     2101 aggtcgt
//
LOCUS       DATADN010000261         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_335078, whole genome shotgun sequence.
ACCESSION   DATADN010000261 DATADN010000000
VERSION     DATADN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335078"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /gene="glgX"
                     /locus_tag="VHH53_03485"
     CDS             <1..1745
                     /gene="glgX"
                     /locus_tag="VHH53_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX2289260.1"
                     /translation="ALGYRDDPAGPRSEVDSLGSVPVSVVTAPGGPDTGTRLDVPWEQ
                     TVLYELHVRGFTRTHPQVPPEHRGTYLGLAHPTVISYLIGLGITAVELLPVSASCAEP
                     ALLRAGSVNYWGYSPLALLAVHPGYAAVPGAEVEEFRTMVAALHAAGIEVIVDVVANH
                     TCEGGVDGPTLAYRGLDARAYYLERDLTGCGNTLDAGSPTVVRLVSDALRYWAGELGV
                     DGFRLDLASVLGRPGGGRFRPDAPLLTAIASDPLLCTCKLIAEPWDATADGYRIGGFP
                     ADFSEWNGRYRDVVRDFWRGVPGVSELASRLAGSSDLYAASGRRPWASVNFVTAHDGF
                     TLRDLVSYNRKHNEANGEGGADGTDDNRSWNCGVEGDTDDPVVLSLRSRQARNLLATL
                     ALSTGTPMLLAGDERWRTQGGNNNPYRLDDETSWVDWSADPETRTLIAFTRRLLALRA
                     MTPALRQPEFFEGRTTPSGTPDLVWFRPDGQPMELDDWRDSHRQTLGMWINGDDVRSH
                     PPEPATDSWLLVLHAGAHPIMVTLPGPECGENYAPVLDTGTPDGVPTRRRSRRAGTRM
                     TVPARTLLLFRAVR"
BASE COUNT          280 a          681 c          619 g          282 t
ORIGIN      
        1 cagcactggg ttaccgggac gatcccgctg gcccccgctc cgaggtcgac tcgctgggca
       61 gcgtcccggt gtcggtggtc accgcgcccg gtggcccgga caccggcacc cggctcgatg
      121 tgccgtggga gcagaccgtg ctctacgagc tgcacgtccg gggcttcact cgcacccatc
      181 cgcaggtgcc gcctgagcac cgcggcacct atctggggct ggcgcacccc accgtgatct
      241 cctacctcat cggtctaggg atcaccgctg tggaactgct gccggtgtcc gcgtcctgcg
      301 ccgaaccagc cctgctgcgc gccggatcgg tcaactactg gggttactcc ccgctggccc
      361 tgctcgcggt gcatcccggg tacgccgcgg tgcccggcgc tgaagtggaa gagttccgca
      421 ccatggtggc cgcgctgcac gccgccggca tcgaggtcat cgtcgacgtg gtggccaacc
      481 acacctgcga gggcggcgtg gacggaccca ccctggccta tcgggggctg gacgcgcgcg
      541 cctactacct ggagcgcgac ctcaccggct gcggcaacac cctggatgcc ggctccccca
      601 cggtggtccg gctggtcagc gacgcgctgc ggtactgggc gggcgagctg ggagttgacg
      661 ggtttcggct ggatctggcc agtgtgctgg gccggcctgg gggtggacga tttcgcccgg
      721 acgcgccgct cctcacggcg atcgcctccg atcccctgct gtgcacctgc aagctcatcg
      781 ccgagccatg ggacgccacc gccgacgggt accggatcgg tgggttcccc gccgacttca
      841 gcgagtggaa cgggcgctac cgcgacgtcg tgcgcgactt ctggcgcggt gtccccggcg
      901 tcagcgagct cgcctcgcgg ttggcaggca gctcggacct ctacgcggcg agcgggcgcc
      961 ggccgtgggc gtcggtcaac ttcgtcaccg cccacgacgg gttcacgctg cgcgacctgg
     1021 tctcctacaa ccgcaagcac aacgaggcca acggtgaagg cggagccgac ggcaccgacg
     1081 acaaccgttc ctggaattgc ggcgtcgaag gcgacacgga cgatccggtg gtgctcagcc
     1141 tgcggagtcg ccaggcccgc aacctgctgg cgaccctggc actatccacc ggcactccca
     1201 tgctgctggc tggtgacgag cgctggcgca cccaaggcgg caacaacaat ccctatcggc
     1261 tcgatgacga gacgtcctgg gtggactggt cagcagatcc cgagacccgc acgctgatcg
     1321 ctttcacccg ccgcctgctc gccctgcgcg caatgacgcc cgcgctgcgc caacccgagt
     1381 tcttcgaagg ccgcacgacc cccagcggca cccccgacct cgtctggttt cgccccgacg
     1441 gccaacctat ggagctcgac gactggcgag acagccaccg gcagaccctt ggcatgtgga
     1501 tcaacggcga cgacgtgcga tcgcacccgc cggaaccggc gaccgactcg tggctgctcg
     1561 tgctgcacgc cggtgcccac ccgatcatgg tcaccctgcc aggaccggaa tgcggcgaga
     1621 actacgcgcc ggtgctggac accgggacgc cggacggagt gcccacccgg cggcgaagca
     1681 ggcgggcagg cacccggatg actgtcccgg cccggacgct cctgctcttc cgagcggtgc
     1741 gctagcccgg cccgatccat gatcactgaa cggccgcctt aatttgcgcg atcaccgtct
     1801 gcggtctgga tgtcaggtca tgccaggtga agcgcagcac cgtccatccg gcaaggatca
     1861 gc
//
LOCUS       DATADN010000262         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1233059, whole genome shotgun sequence.
ACCESSION   DATADN010000262 DATADN010000000
VERSION     DATADN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233059"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VHH53_03490"
     CDS             complement(<1..556)
                     /locus_tag="VHH53_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048040.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX2289261.1"
                     /translation="MTASIEQRPRSYRVRTYGCQMNVHDSERLAGLLESAGYVPAGPD
                     DTPDVVVLNTCAVRENADNRLYGNLGQLRPVKNEHPGMQIAVGGCLAQKDRDEITRRA
                     PWVDVVFGTHNIGALPVLLDRARHNGIAQVEIAESLEVFPSALPAKRDSAHSAWVSLS
                     VGCNNTCTFCIVPALRGKEVDRRPG"
     gene            825..1553
                     /locus_tag="VHH53_03495"
     CDS             825..1553
                     /locus_tag="VHH53_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2289262.1"
                     /translation="MIRMSGVDKYFGSLHVLRDINLDVARGEVVVVLGPSGSGKSTLC
                     RTINRLEPIDGGRIEVDGAPLPAEGRALARLRADVGMVFQQFNLFAHKTIAENVQLAP
                     VRVRRISKRQAHEDAMRLLERVGITDQADKYPAQLSGGQQQRAAIARALAMRPKVMLF
                     DEPTSALDPEMVQEVLDVMTSLAREGMTMLVITHEMGFARRAAHRVAFMSGGEIVEDA
                     VPEQFFTTPRSVRAQNFLGKILTH"
     gene            1610..>2198
                     /locus_tag="VHH53_03500"
     CDS             1610..>2198
                     /locus_tag="VHH53_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX2289263.1"
                     /translation="MKLRDMAVGLLVSSLVLISCGRETSPGASGTEVPAPSVAHDVTV
                     TGSPTFDRMTQRGTVIIGVKNDQPGLGYLNPTTNRYEGFDIEISRLIAAKLGFDPETK
                     IEYKAIPSAAREQAIITGQIDYYVGTYTINDARKEQISFAGPYFVAGQDLLVRSDDNS
                     ITGPDTLAGKRVCSATGSTPIRRIREQGLTDQVIEF"
BASE COUNT          396 a          702 c          708 g          392 t
ORIGIN      
        1 caccgggccg gcggtcgacc tctttgccgc gcagcgcggg cacgatgcaa aaagtgcagg
       61 tgttgttgca gcccacggag agcgacaccc acgccgagtg cgccgagtcc cgcttcgcgg
      121 gtagcgcgga agggaaaacc tccagcgact ctgcgatctc gacttgagca atcccgttgt
      181 gccgggctcg atccaacaac accggtagcg caccgatgtt gtgggtaccg aacaccacgt
      241 cgacccaggg cgcccggcgg gtgatctcgt cgcggtcctt ctgcgccagg cagccaccga
      301 ccgcgatctg catcccaggg tgctcattct tgaccgggcg tagctggccg aggttgccgt
      361 agagccggtt atcggcgttt tcgcgcacgg cgcaggtgtt cagcacgacc acgtcggggg
      421 tgtcatccgg cccagcgggg acgtatccgg cgctctcgag gagcccggcc aaccgttcgg
      481 agtcgtgcac attcatctgg cagccgtagg tgcgcacgcg gtagctgcgc gggcgctgct
      541 cgatgctggc cgtcatcgat cattcctctg tgcaggttgc tgtatgggcc tgcgttggtg
      601 tgctgcggtg tggcccgtcg aaaggttcgt gccccctcca gagtagagcc gggcccaaac
      661 ctgtcctccg gctgcccggg tcatcaacgg tggccgggat gtaccgccat gggcgattcg
      721 tgacaaagtt gcactctgcg tcccgcaccc tgtcgggtag ggatagtgca gtgctcccgc
      781 acccggagga ccggatgagt tcacccgcga cagccgaccc gccgatgatc aggatgtcgg
      841 gagtggacaa atatttcgga tctctgcacg tgctgcgcga catcaatctg gacgttgccc
      901 ggggcgaggt ggtcgtggtg ctcggaccat caggatccgg caagtcgacg ctatgccgca
      961 cgatcaaccg actggagccg atcgacgggg gccgtatcga ggttgacggg gcgccgctgc
     1021 ctgcggaggg tcgggcgctg gcccggctgc gagccgatgt cgggatggtc tttcagcagt
     1081 tcaacctttt tgctcacaag accatcgcgg agaacgtgca gctcgctccg gtgcgcgtac
     1141 gccggatcag taagcggcag gcccacgaag atgcgatgcg gttgctggag agagtcggaa
     1201 tcaccgacca ggctgacaaa tacccggcgc agctgtcggg cggccagcag cagcgggctg
     1261 cgatcgctcg cgcgctggcc atgcggccca aggtaatgct tttcgacgag ccgacctcag
     1321 cacttgaccc agagatggtc caggaggtgc tcgacgtcat gacctcactg gcccgcgaag
     1381 gcatgaccat gcttgtgatc actcacgaaa tgggtttcgc ccggcgcgcc gcgcatcggg
     1441 tggcgttcat gtccggcggc gagatcgtcg aggacgcggt gcctgagcag ttcttcacca
     1501 caccgcgctc ggttcgcgcc cagaactttc tcggcaagat tctgacgcac tgatgcccga
     1561 ccatcggcgt caaccgatgc ctgcgtcgaa ctaccggatg gagtagcaca tgaagctgcg
     1621 cgacatggcg gtcggcctgc tggtcagtag cctcgtattg atcagctgcg gccgggagac
     1681 gtctccggga gccagcggca ccgaggttcc ggcgcccagc gtggcacacg atgtcaccgt
     1741 caccggttcg cccacctttg accggatgac ccagcgcggc accgtgatca tcggcgtcaa
     1801 gaatgaccag cccgggctgg gctacctcaa cccgacgacc aacaggtacg agggcttcga
     1861 catcgagatt tcccggctca tcgcggccaa gctcgggttc gatccagaaa ccaagatcga
     1921 atataaggcg atcccgtccg cggcacgcga gcaggcgatc atcactgggc agatcgacta
     1981 ttacgtcggc acctatacga tcaatgacgc ccgtaaggag cagatttcct tcgccggccc
     2041 ctatttcgtc gccggtcagg acctgctggt cagatctgac gacaattcta ttaccggacc
     2101 cgatacgctc gcgggcaagc gggtgtgctc ggccaccgga tcaaccccca tccgccggat
     2161 tcgcgagcaa ggcctcacag accaggtcat cgagttcc
//
LOCUS       DATADN010000263         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_484403, whole genome shotgun sequence.
ACCESSION   DATADN010000263 DATADN010000000
VERSION     DATADN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484403"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1605
                     /gene="acnA"
                     /locus_tag="VHH53_03505"
     CDS             <1..>1605
                     /gene="acnA"
                     /locus_tag="VHH53_03505"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX2289264.1"
                     /translation="PQDRIALSDAKTAFRRALADYASVAQSAEAGSSEVDHAGEESFP
                     ASDSPAVSRGDGDGVPRTTISHAAGAPDRLSCPTPVHTGEDAGYEIDHGAVVIASITS
                     CTNTSNPSVMIGAALLARNAVEAGLTVKPWVKTSLAPGSQVVTDYYEKAGLWPYLEKL
                     GYHLVGYGCTTCIGNSGPLPEEVAHAVRDADLAVVSVLSGNRNFEGRINPDVSMNYLA
                     SPPLVLAYALAGTMDVDFEHDPLGFDRDGNKVYLTDIWPSPEEIQKVIESAISQEMFV
                     KDYADVFAGDSRWRSLPTPQGEIFSWDPESTYVRKPPYFDDMAAQPPSVADIGGARVL
                     ALLGDSVTTDHISPAGAIKEDSPAGRYLSEHGVARKDFNSYGSRRGNHEVMIRGTFAN
                     IRLRNLLLDAISDSPVSGGYTRDFTVVSGESGESDGGQPVSIYDAAQRYAERGIPLLI
                     LGGKEYGSGSSRDWAAKGTRLLGVRAVIVESFERIHRSNLIGMGVLPLQFPAGESASS
                     LGLDGTETFDISGVTALNNGSIPPTV"
BASE COUNT          278 a          535 c          507 g          285 t
ORIGIN      
        1 gcccgcagga tcgcatcgca ttgtcggacg ccaagaccgc atttcgccgc gcgctggctg
       61 actatgcttc cgtagcgcag tctgcggagg caggaagctc cgaggtggac cacgccggcg
      121 aggaatcctt cccggccagc gattcacccg cggtgagtcg cggcgacggc gacggggtgc
      181 cccgcacgac gatctctcac gcggccggtg cgccggaccg gctcagctgt cccacaccag
      241 tgcacaccgg cgaggacgcc gggtacgaga tcgaccacgg tgcggtggtg atcgcctcga
      301 tcacctcgtg caccaacacc tccaacccgt cggtgatgat cggcgcagcg ctgctggccc
      361 gcaatgccgt cgaggcgggg ctgaccgtca agccatgggt gaagacgtcg ttggctcctg
      421 ggtcccaagt tgtcaccgat tactacgaga aggccggcct gtggccctac ctggagaagc
      481 tggggtacca cctggtcggc tacggctgca ccacctgcat cggcaactcg ggcccgctgc
      541 ccgaagaggt cgcacatgcc gtccgcgacg ccgaccttgc agtggtcagc gtgctgtcgg
      601 gcaaccgaaa cttcgagggt cggatcaacc cggacgtctc gatgaactac ctcgcctccc
      661 cgccgttggt gctcgcctac gcactggccg gaacgatgga cgtcgacttc gagcatgatc
      721 cgctagggtt cgatcgcgac ggcaacaagg tctacctgac tgacatctgg ccctccccgg
      781 aggaaatcca gaaggtgatc gagtccgcga tcagccagga gatgttcgtt aaggactacg
      841 ccgacgtctt cgccggtgat tcccgctggc ggtccctacc gaccccgcaa ggcgagattt
      901 tcagctggga tccggagtcc acctacgtcc gcaagccccc gtacttcgat gacatggccg
      961 cgcaaccgcc gtcagtggcc gacatcggtg gggcccgggt gctggcactg cttggtgact
     1021 cggtgacgac cgaccacatc tctcccgcag gggcgatcaa ggaagactct ccggccggcc
     1081 ggtacctgtc cgagcatggt gtggcgcgca aggatttcaa ttcctatgga tcccgccggg
     1141 gcaatcacga ggtgatgatc cgcggcacgt tcgccaatat ccggttgcgc aacctgctgc
     1201 tcgacgccat ctccgacagc ccggtcagcg gcggttacac ccgcgacttc actgttgtat
     1261 ccggagagtc aggggaatcc gatggtggcc aaccggtgtc aatctatgac gccgcgcagc
     1321 gttacgccga gcgcggcatt ccgctgctga tcttaggtgg caaggaatac ggttcgggct
     1381 cgtcacggga ctgggctgcc aagggcaccc ggctgctcgg ggttcgcgcg gtgatcgtcg
     1441 agtcattcga acgcattcac cgttccaacc tcatcggaat gggcgtgctt cccttgcagt
     1501 tcccggctgg tgagtcggcc tcgtcgctgg gtctggacgg caccgagacc ttcgacatct
     1561 ccggcgtcac ggcgctcaac aacggctcca tcccgccgac agtgc
//
LOCUS       DATADN010000264         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1388717, whole genome shotgun sequence.
ACCESSION   DATADN010000264 DATADN010000000
VERSION     DATADN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388717"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VHH53_03510"
     CDS             <1..140
                     /locus_tag="VHH53_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX2289265.1"
                     /translation="QCGPHRVVSLMSSEAVRELGLAPGVLAVAVVKSTEVMVETPGGR
                     A"
     gene            137..910
                     /gene="modA"
                     /locus_tag="VHH53_03515"
     CDS             137..910
                     /gene="modA"
                     /locus_tag="VHH53_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2289266.1"
                     /translation="MKRWVMVGLAVGLALAGCGTGDTAQTEPDGQDRVPVTVLAAASL
                     TDVFSKLEPAFEAANPGADLRFSFGASSDLASQIVNGAPADVFASANEKQMKVVADAG
                     KVAGESKPFTTNVLTIVVPPGNPKGIQSFADLARPDVTELVCAPQVPCGAATEEIEQA
                     TNIELSPASEEPDVRSVLAKVQAKEADAGLVYITDARNAAGTVEQINFPEAKDAVNVY
                     PIAAVEGAPHPQLAEAFVAFVLGPEGQRELTAAGFSPAS"
     gene            907..1719
                     /locus_tag="VHH53_03520"
     CDS             907..1719
                     /locus_tag="VHH53_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289267.1"
                     /translation="MTQTRDPHAVRTGLPGGLWVPAVAALGLIVLPVIGLVVRAPWPR
                     FVELITSDSALAALELSLRTAVVSTLLCVVLGGPLAVVLARGSVPGLRLLRSVVLLPL
                     VLPPVVGGLALLYLLGRSGFAGYALEVGFGLRLPFTTAAVILAQTFVAMPFLVVSLEG
                     ALRTGGERYEVIAASLGAPPWTVFRRVTVPLVLPGLGSGMVLSFARCLGEFGATIAFA
                     GSLQGVTRTLPLEVYLQREIDADAAIALSLLLVVVAIAVIAVARPRSAEGLG"
BASE COUNT          256 a          583 c          686 g          336 t
ORIGIN      
        1 tccagtgcgg accacatcgg gtggtctcac tgatgagcag cgaagcagta cgcgaactgg
       61 ggctcgcgcc gggggtgctg gcagtggctg tcgtgaagtc gaccgaggtc atggtcgaga
      121 ccccaggagg acgagcatga agcgatgggt catggtgggg ctggcggttg ggctggcgct
      181 ggccggctgt ggaacaggtg atacagccca gaccgagccg gatggccagg accgggtacc
      241 ggtcacggta ctggcagcag cctctctcac cgacgtgttc tccaagctgg agccagcgtt
      301 cgaggccgct aatcccggtg ctgacctgcg attcagcttc ggcgcctcgt cagatctggc
      361 gagccagatc gtcaacggcg cccctgccga tgtattcgct tcggccaatg aaaagcagat
      421 gaaggtagtc gccgacgcgg ggaaggtggc cggcgaatcg aagccgttca ccaccaacgt
      481 gctgaccatc gtggtgcccc cgggaaatcc caagggcatt cagtcgttcg ccgatctagc
      541 caggccggat gtgaccgagt tggtgtgtgc tccgcaggtg ccctgtggcg cggccacgga
      601 ggagatcgag caggccacca acatcgagct gagcccggcc agtgaggagc ctgacgtcag
      661 gtcggtactg gccaaggtcc aggccaagga agccgacgcc gggctggtct acatcaccga
      721 tgcccgcaac gccgccggga cggtcgagca gatcaacttc ccggaggcca aggacgcggt
      781 caacgtctac ccgatcgccg cggttgaggg ggccccgcat ccgcagctcg cggaggcctt
      841 cgtggcgttc gtcttgggtc ccgaaggtca gcgagagttg accgccgccg gcttcagccc
      901 tgcttcttga cccagacaag ggacccgcac gccgtccgta ccgggctacc cggtgggctg
      961 tgggtgcccg cggtagcggc gctcgggttg atcgtgctgc cggtgatcgg gctggtggtg
     1021 cgggcgccgt ggccccggtt tgtcgagctg atcaccagcg actccgcgct ggccgcgttg
     1081 gagctgtcgc tgcgtaccgc agtggtgtcc acgttgctgt gtgtcgtgct cggtgggccg
     1141 ctggcggtgg tgctggcccg cggatcggtg cccggtctgc ggttgctgcg ctcggtcgtg
     1201 ctgctcccgc tggtcttgcc gccggtggtc ggcgggctgg cgctgctgta cctgttgggc
     1261 cgtagcggat tcgccggcta tgccctcgag gtgggtttcg gcctgcgcct gccattcacc
     1321 accgcggcgg tgatcctggc gcagacgttc gtggcgatgc cgtttctcgt ggtgagtctg
     1381 gagggtgcgc tgcgcaccgg tggggagcgt tacgaagtga tcgcggcgag cctcggtgcg
     1441 ccgccgtgga cggtgttccg gcgggtcacc gtcccgctgg tgttgcctgg actgggctct
     1501 ggcatggtgc tcagcttcgc gcgctgcctg ggcgagttcg gtgccaccat cgccttcgcg
     1561 ggcagcctgc agggtgtcac tcgcacgctt ccgctggagg tctacctgca gcgcgagatc
     1621 gacgccgacg cggcaatcgc gctgtcgctg ctgctggtag tggtggcgat cgcggtgatc
     1681 gcagtagccc ggccccgctc ggcggagggc ttgggatgag cgcggggttg caggtcgagg
     1741 tcgcggtacg ccgcggggac ttcgcgctca tccccaaccc tccgccgagc ggggccgggc
     1801 tactgcgatc accgcgatcg ccaccgccac cagcagcagt gacagcgcga ttgccgcgtc
     1861 g
//
LOCUS       DATADN010000265         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_335766, whole genome shotgun sequence.
ACCESSION   DATADN010000265 DATADN010000000
VERSION     DATADN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335766"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VHH53_03525"
     CDS             <1..683
                     /locus_tag="VHH53_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX2289268.1"
                     /translation="LPVGSEDAARHYAAAAIEAGAAFVNCMPAFIASDLGWARRFAEA
                     GVPIVGDDIKSQVGATITHRVLAKLFEDRGVELVRTYQLNFGGNMDFLNMLERKRLLS
                     KKISKTQAVTSQIPHEMGKADVHIGPSDHVPWLDDRKWAYVRLEGRTFGDVPLNLEYK
                     LEVWDSPNSAGVIIDAVRAAKIAKDRGFGGPILSASSYFMKSPPEQYSDSEAYQAVEA
                     FIRGEVDQ"
     gene            749..1510
                     /locus_tag="VHH53_03530"
     CDS             749..1510
                     /locus_tag="VHH53_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX2289269.1"
                     /translation="MSSNRGSRGQRGEPSTSDQGTAAGEDQLAQQLSMLARQLQEKQS
                     LQETLSGIVHATVDNVPGAQFAGLTVVKGRREVHTAAWTDELVRTADQVQYDTGQGPC
                     LNSVYEEATVRVTDMRSEQRWPEFAKRAVDLGIKSMLSIQLYVTGDNLGALNLYSQAI
                     EAFNDESEHVGLLLAAHAAIAMAGAQQHEQLVQAITTRDLIGQAKGILMERHKITAEQ
                     AFTLLIRASQLGNAKLRDVAEHLTTTGELAARQRQ"
     gene            1745..2065
                     /locus_tag="VHH53_03535"
     CDS             1745..2065
                     /locus_tag="VHH53_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2289270.1"
                     /translation="MALIGELDLASAPHLQRVLDELRRDGSQEIVLDLARLDFLSATG
                     LEVFVRADEHLRAAGGRLVLNRPGRRARRILAITGLDAVLTIRPGTSGGLDATVTSGV
                     SQAS"
     gene            complement(1967..3124)
                     /locus_tag="VHH53_03540"
     CDS             complement(1967..3124)
                     /locus_tag="VHH53_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX2289271.1"
                     /translation="MKRRKILAHGAAVTLGTTLAFGTTVMSGTAGLAADSAQWLARPT
                     QKPAPSNIGMTDVKQVEAVTSAMRTLDYQFGGGACRDAVVAQLSWAQRLLSASSTDGV
                     RARLFRALADMQNLAGWTAFDVGLLDPSRQHFTIALQYARQCADPDLLSNIMYRIGRV
                     FLHHGAPHEALSWFQLGQRPAQDSGSALAVAVLCSNEAWAYAVMGDDVQARKLLGRSQ
                     DELARADLSEAPDWARFYNETDMNAMIGTVHTVLSAIDLKHAAIAIPAINQSLAHYDE
                     SMSRSRAFMLTALATNHLRQGDVDHGVRVGRDALTLATGLQSKRVTDRLRPLELAAGK
                     SNDSDSRQLAHLIRLHSSPSGRLANPAGDGSVQAARGPWPDRQDSVQSSDR"
     gene            complement(3135..3506)
                     /locus_tag="VHH53_03545"
     CDS             complement(3135..3506)
                     /locus_tag="VHH53_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289272.1"
                     /translation="MTPVLAAVWCRSVGSTWTIELHKLDRGTALGTTVDWISSGVPTS
                     QRQPHEDMARELLASRGLHLYPDPTAGPCTHSRYSIGYVSAQVQVIVLADRLREVAAT
                     AGVHPVTLAARRIAAEFSPMG"
BASE COUNT          647 a         1243 c         1262 g          597 t
ORIGIN      
        1 acctccccgt cgggagcgag gacgctgcga ggcactacgc cgcggcggcg atcgaggcag
       61 gcgccgcgtt cgtcaactgc atgccggcct tcatcgcctc ggacctcggc tgggcccggc
      121 ggttcgccga ggccggcgtc cccatcgtgg gcgacgacat caagtcgcag gtcggggcga
      181 ccatcacgca ccgggtgctg gccaagctgt tcgaggaccg tggggtcgag ctggtgcgca
      241 cctaccagct caacttcggc ggcaacatgg acttcctgaa catgctggag cgcaagcggc
      301 tcctgtcgaa gaagatctcc aagacgcagg cggtcacctc gcagatcccg cacgagatgg
      361 gcaaggccga cgtgcacatc ggcccgtccg accacgtgcc gtggctcgac gaccgcaagt
      421 gggcctacgt ccggctggag ggtcggacgt tcggcgacgt gccgctgaac ctggagtaca
      481 agctcgaggt gtgggactcc cccaactccg ccggcgtgat catcgacgcg gtgcgcgccg
      541 cgaagatcgc caaggaccgc ggcttcggcg gcccgatcct gtcggccagc tcctacttca
      601 tgaagtcgcc gccggagcag tactccgact ccgaggccta ccaggccgtc gaggcgttca
      661 tccgcggcga ggtagaccag tagggtcgaa cgactgcgac tgggaatacc ggtactgact
      721 cgacgcagct tgacggcatg attgactcat gagttctaat cgcggttccc gcggccagcg
      781 gggggagccc tcgacgagcg accagggaac cgccgcgggt gaggaccagc tagcccaaca
      841 gctcagcatg ctggcccgtc aactgcagga gaagcaatcg ctacaagaaa ccctcagcgg
      901 catcgtgcac gccacggtgg acaacgtgcc cggagcccag ttcgccgggc tgaccgtggt
      961 caagggccgc cgggaggtgc acacagcggc gtggaccgac gaattggttc gcacagccga
     1021 ccaggtgcag tacgacaccg gccagggacc gtgcctgaac tccgtctacg aggaggcaac
     1081 cgtccgggtg actgacatgc gctccgaaca gcggtggcca gagttcgcca agcgagccgt
     1141 cgatctcggc atcaagagca tgctgtccat ccagctctac gtcacgggcg acaacctcgg
     1201 cgctctcaac ctctacagcc aggctatcga ggcattcaac gacgaatccg agcacgtcgg
     1261 cctcctgctc gccgcgcacg ccgccatcgc gatggctggt gcccagcagc acgagcagtt
     1321 ggtgcaggcg atcaccaccc gcgatctcat cgggcaggcc aaaggcatcc tgatggagcg
     1381 ccacaagatc accgccgagc aggcgttcac actcctgatc cgggcaagcc agctggggaa
     1441 cgccaagttg cgcgacgtcg ccgaacatct caccactacc ggtgagctgg cggccaggca
     1501 gcgccaatag gtgcagttgc gcgccgtggg ttgctgcgcg tggcgctgtt tgcctattgt
     1561 cggtacaagg ttgagccatc agccgctgcg gcagcacacc agcgggtggc cacctgagtc
     1621 ccccgcggca ctgacacaga cgggggcgcc gataatgcgc aacagctcga ggatgaggcc
     1681 ggtccacaac ggtcgcggac cgatgttcgc gctggaaatc cgcccggatt gccactgcag
     1741 tcaggtggct ttgatcggag agctcgacct ggccagcgcg ccgcatctgc agcgggttct
     1801 cgatgaactg cgccgggatg gctctcagga gatcgtgctc gacctggcca ggctggactt
     1861 cctcagcgcc accggcctgg aagtgttcgt ccgcgccgat gagcacctac gcgccgccgg
     1921 cgggcgactc gtcctcaacc ggcctgggcg gcgggcccgt cgcatcctag cgatcactgg
     1981 actggacgct gtcttgacga tccggccagg gacctcgggc ggcctggacg ctaccgtcac
     2041 cagcggggtt agccaggcgt cctgacggcg agctgtgcag gcggatcaga tgggcgagct
     2101 ggcgggaatc cgagtcattc gacttccctg ccgcgagttc caacggtctc agccggtcgg
     2161 tcacccgctt ggactgcagt ccggtggcca gggtcagggc gtcccgaccg acccggacac
     2221 cgtggtcgac gtcgccttgg cgcagatggt tggtggccag cgcggtgagc atgaaggccc
     2281 ggctacggct catcgactcg tcgtagtgag ccaaggattg gttgatcgcg gggatggcga
     2341 tggccgcgtg cttgaggtcg atcgccgaca gcacggtgtg cactgttccg atcatcgcgt
     2401 tcatgtcggt ctcgttgtag aaccgggccc agtcgggcgc ctcggacaga tcggccctgg
     2461 ccaactcgtc ctgtgatcgg ccgagcagct tcctggcctg cacatcgtcg cccatcaccg
     2521 cgtaggccca ggcttcgttg ctgcacagca ccgcgacagc cagggccgat ccggagtcct
     2581 gcgctgggcg ctggcccagc tgaaaccagc tgagtgcctc gtgcggggct ccgtggtgca
     2641 ggaagacccg gccgatccgg tacatgatgt tggacagcag gtccgggtct gcgcattgcc
     2701 tggcgtactg cagtgcgatg gtgaagtgct ggcgcgaagg gtccagcaac cccacgtcga
     2761 aggcggtcca tccggccagg ttctgcatgt cggccagcgc ccggaaaagc cgcgcccgta
     2821 ccccgtcagt gctcgacgcg gacagcaggc gttgtgccca ggacagttgg gccaccaccg
     2881 cgtcacggca cgcgccgccg ccgaactggt agtccagggt gcgcatggcg ctggtgacgg
     2941 cctcaacttg cttgacatcg gtcataccga tgttgctggg cgccggcttc tgggtagggc
     3001 gggccagcca ttgagcgctg tccgcggcca gccccgcagt gccggacatc actgtggtgc
     3061 cgaaggccaa cgtggtgccg agcgtcaccg cggctccgtg tgccaggatc tttcggcgct
     3121 tcaccgactc ctcctcagcc cataggggag aactcggccg cgatgcgtcg cgcggccagc
     3181 gttacggggt ggactccagc cgtggcagcg acctcgcgta ggcggtccgc caagacgatc
     3241 acctggactt gggcggatac gtagccgatg ctgtaccggc tgtgcgtaca ggggcccgcg
     3301 gtcggatccg ggtagagatg cagcccccgg ctagcgagca actcgcgggc catgtcctcg
     3361 tggggttggc gctgggatgt cggcacgccc gagctgatcc aatcgaccgt cgtccccagc
     3421 gccgtgccgc ggtccagctt gtggagctcg atcgtccagg tgcttcccac cgaacgacac
     3481 cacaccgccg cgagcaccgg cgtcaccgca gttggcgtgc tgggcgagcg accgcgtaac
     3541 ccggaccggc acggatctcg aacaagatcc gaaagcgcgc tgacagagtg gtctggcggc
     3601 atgccgctgc acccccacga gtggctgtgg tggccgcgct actggaccgc aaccccaacg
     3661 gtaacaagaa aatcctgcgc aggcttgtcg gcgtttcagt gagtccgcag cggggtaggc
     3721 ggtggtatcc caccccacca cagtttgga
//
LOCUS       DATADN010000266         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_938836, whole genome shotgun sequence.
ACCESSION   DATADN010000266 DATADN010000000
VERSION     DATADN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938836"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VHH53_03550"
     CDS             <1..985
                     /locus_tag="VHH53_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2289273.1"
                     /translation="LLPEYRNPGSRRVDPGSVVLSLAAILPVVYGLKESATHGLGWLP
                     ALSIVAGLTTGALFIRRQRTLADPMLDLQLFGDRAFSAAVTTHLLSLFLVAGIQYLIA
                     QHLQLVLGLPPLQAGLWTLPAAVGGLVGAMLAPVLIRAASPSGVITAGLVTGAVGLGI
                     LTQIDAHAGLPVLVAGFIITSFAVGLVTALTTDLIIGSAPPERAGAASGISETSAELG
                     IALGIAVLGSIGTAVYRNQVAGAVLAGVPPDAAQATRETLGGAVAAANQLPGERGVEL
                     LNTAREAFTQGLQVNAGLSALVAIGLAIMSATLLRHTSTRWSPRIMQSSGV"
     gene            1043..1429
                     /locus_tag="VHH53_03555"
     CDS             1043..1429
                     /locus_tag="VHH53_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2289274.1"
                     /translation="MSVELNHTIVAARDKKAAADFLASILGLEVGAPFGPFLPVETSN
                     SVTLDFMDSDADQIVPQHYAFLVSEEDFDAIFARIQQAGIAFYADPGRHEAGQINHND
                     GGRGAYFTDPNGHILEIITRPYGSGE"
     gene            1476..>1934
                     /locus_tag="VHH53_03560"
     CDS             1476..>1934
                     /locus_tag="VHH53_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2289275.1"
                     /translation="MLAEAAYRARRYVDSVDHRPVAPAPAALEGLDRFDEPLPENSSD
                     PLETLSLLDEAGSDATVATTGPRYFGFVNGGTLPIALACGWLGMAWDQTAALPVMSPV
                     AARLHEVTRNWLVQLFGLPYTSEAAFVTGATMANVTALAAARDHQLARLGW"
BASE COUNT          309 a          656 c          624 g          345 t
ORIGIN      
        1 actactgccc gagtaccgaa accccggatc gcgtcgggtg gacccgggca gcgtcgtcct
       61 gtccctggct gccatcctgc cggtggtcta cggcctcaag gaatccgcca cacacggcct
      121 ggggtggcta cccgcgctgt ccatcgtggc cggcctcacc accggcgcgc tgttcatccg
      181 ccggcagcgc acgcttgccg atccgatgct cgacctgcag ctgttcggcg accgcgcctt
      241 cagtgcggca gtgaccacgc acctgttgtc cctgttcctc gtggccggga tccaatacct
      301 catcgcgcag catctgcagc tggtgctcgg actgccgccc ctgcaggcag ggttgtggac
      361 cttgcccgca gccgtcggag ggctcgtggg tgcgatgctg gccccggtcc tgatccgagc
      421 cgcctccccg tcgggggtga tcaccgccgg tctggtgacc ggagccgtcg gcttggggat
      481 cctcacccag atcgacgcgc acgccggcct tccggttctg gtcgccgggt tcatcatcac
      541 gtcgttcgcc gtcgggttgg taacggccct gaccaccgac ctgatcatcg ggtcggcgcc
      601 tccggaacgg gccggggccg cgtcggggat ctcagagacc agcgccgagc tcggtatcgc
      661 gctgggcatc gcggtgctgg gcagtatcgg caccgccgtc taccgcaacc aggttgccgg
      721 cgctgtcctg gccggcgtcc cacccgacgc ggcgcaagcc acccgggaaa ccctgggagg
      781 cgcagtggcc gcggccaacc agctacccgg cgaacgtggc gtcgagctgc tcaacaccgc
      841 ccgcgaggcg ttcacccaag gactgcaagt caatgccggg ctcagcgccc tggtcgcgat
      901 cggccttgcc atcatgtcag cgaccctgct gcgccacacg tccacgcgct ggagtccccg
      961 cataatgcag tcgagcggcg tctagcgaac aatgagtact gaactaactg aacgatgaca
     1021 ccctggagac cctggagaat ccatgtccgt cgagctgaat cacacgatcg tcgcagcccg
     1081 cgacaaaaaa gcagccgctg atttcctggc cagcatcctg gggctggagg tcggcgctcc
     1141 tttcggcccg ttccttcccg tcgaaaccag caacagcgtt actttggact tcatggacag
     1201 cgacgcggac cagatcgtcc cgcagcacta cgcgttcctg gtgtccgaag aggatttcga
     1261 cgccatcttc gcccgtatcc agcaggccgg tattgccttc tacgccgatc ccggccgcca
     1321 tgaggccggg cagataaacc acaacgatgg tggccgcggc gcatatttca cggacccgaa
     1381 cgggcacatt ctggagatca tcacccgccc gtacggaagc ggcgagtgag cttgacgtgg
     1441 tgaacggttc ctcggcgtgg gctggacgtg acgagttgct cgcggaggcg gcataccgtg
     1501 cgcgtcgata cgtcgacagc gtggaccacc gtccggtggc gccggcaccc gcggcgctgg
     1561 agggtctgga tcgcttcgat gaaccgctgc cggaaaactc gtcggatccg cttgaaacgc
     1621 tgtcgttact ggatgaggcc ggctcggacg cgacggtggc cacgaccggt ccgcggtatt
     1681 tcgggttcgt caacggcggt acgttaccga tcgcattggc ctgcggatgg cttggtatgg
     1741 cgtgggatca aaccgccgct ttgccggtca tgagtccggt ggcggcccgc ctgcatgaag
     1801 tcactaggaa ttggctggtt cagttgttcg ggctgccata caccagtgag gccgcgttcg
     1861 ttaccggggc gaccatggcc aacgtcacag cactggccgc cgcgcgggat catcagctcg
     1921 cccgcctggg ttgg
//
LOCUS       DATADN010000267         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_630583, whole genome shotgun sequence.
ACCESSION   DATADN010000267 DATADN010000000
VERSION     DATADN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630583"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VHH53_03565"
     CDS             <1..265
                     /locus_tag="VHH53_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289276.1"
                     /translation="QLAPTQAETQPPPVINLADYQVRRRPILGGLTRALSSLKGLDLS
                     SCAAPRLVDLFSFVLRRFWAAPVAASGSRIGPVGPTASAAAGP"
     gene            complement(554..1594)
                     /locus_tag="VHH53_03570"
     CDS             complement(554..1594)
                     /locus_tag="VHH53_03570"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2289277.1"
                     /translation="MDESYAGQQVVGLDLHRRRTVMVRMTPAGERLEAVRFASDPAVL
                     AEQIAKAGPSPEVVVEATYNWYWVADVLDEAGARLHLAHPLGVKGFAYRRVKNDFRDA
                     ADLADLLRMGRLPEAWIAPKHVRCQRELVRHRHKLRQLCSGLKAGAHAVLAKNALLIP
                     RSDMFGVDGRHRLRRLALPEAYRVRLDSQLHLIDAFEAEIEDLDGRIRGLFDADAGYR
                     ALLRVPGIGPVFAAILRAEIGEIGRFPSPRQLNSWAGLTPRHRESDTTVHRGAITKQG
                     SRLVRWAAVEAVQRTRAGTPMRARYEQVVTRRGATAKHVAKVAAARELLDCVFYALRD
                     GRVRRLGHTGAA"
     gene            1754..>1934
                     /locus_tag="VHH53_03575"
     CDS             1754..>1934
                     /locus_tag="VHH53_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_052642687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX2289278.1"
                     /translation="MDATLSSYKGHRFPVEIISHCVWLYHRFPLSLRDVQEMMAERGV
                     IVSYESVHQWCRKFGQ"
BASE COUNT          311 a          662 c          644 g          317 t
ORIGIN      
        1 gcaactcgcg ccaactcagg ccgagaccca acccccacca gtgatcaacc tcgccgacta
       61 tcaggtgcgc cgcagaccga tcctcggcgg gctcaccagg gcattgtcat ccctgaaagg
      121 actggacctg tcaagctgtg ccgccccacg gctggttgat cttttttctt tcgtccttcg
      181 ccgcttttgg gcagcgccgg ttgcggcgtc ggggtccagg atcggcccgg tagggccgac
      241 cgcgtcagcg gccgcaggtc cttgacgtcg acgccgtcgt tcggcgcagg cttgtgatgg
      301 cggagaagaa agctggtgag tctccctgga ctgtgggtgg ggttagatga gcagacgcct
      361 gtcgggtctc cagcgcgtgc gcgcatgggt agggcaggcc ctgaaggtgg gctgccggtc
      421 atcccttcgc cgcgtggtgg cggcatggag tgtttcggcg tccccggtgg gtggggccaa
      481 tcaagttaac gggcgcggcg aggcgcccag acaaagcagg gccgcgcacc caccggggtg
      541 gtccgcacgt gtgtcatgcc gcgccggtgt gtcccaggcg gcgtacgcgt ccatcgcgta
      601 gggcgtagaa cacgcagtcg agcagctcgc gggcggcggc gaccttggcc acgtgcttgg
      661 cggtagcccc gcgccgggtg accacctgct cgtagcgggc acgcatcggg gttcccgcac
      721 gggtgcgctg cacggcctcc accgcggccc aacgcaccag ccgtgaacct tgcttggtga
      781 tcgcgccacg gtgcacggtg gtgtcggact cgcggtgacg cggggtcaac ccggcccacg
      841 agttgagctg gcgtggcgag gggaaccggc cgatctcacc gatctcggcc cgcaggatcg
      901 cggcgaacac cgggccgata ccaggaaccc gcagcagcgc ccggtaaccc gcatcggcgt
      961 cgaacagtcc acggatgcgc ccatcgaggt cctcgatctc ggcctcgaag gcgtcgatca
     1021 ggtgtagctg ggagtccaac cgcacccgat atgcctccgg cagcgccaat ctccgcagtc
     1081 ggtgccgccc gtcgacaccg aacatgtccg agcgagggat cagcagcgcg tttttggcca
     1141 gcaccgcgtg cgcgccggcc ttgagccccg aacacagctg gcgcagcttg tgccggtggc
     1201 gcaccagctc gcgctggcac cgcacgtgtt tgggcgcgat ccacgcctcg ggcagtcgac
     1261 ccatccgcag cagatccgcc aggtccgcgg cgtcgcgaaa gtcatttttc acccgccggt
     1321 aggcaaagcc cttcaccccc aacggatgcg ccagatgcag ccgcgccccg gcttcatcga
     1381 gcacatcggc cacccaatac cagttgtagg tggcctcgac gactacctcc ggcgacgggc
     1441 cggccttggc gatctgctcg gcaagcaccg ccggatccga ggcgaaccgc accgcctcca
     1501 accgctcacc cgccggggtc atccgcacca tcaccgtgcg ccgccgatga aggtccaaac
     1561 ccactacctg ttgccctgcg tacgattcgt ccacaagggg cctcctcgag ccgttctggt
     1621 tggcatgagc acccccgagc ctgcgctcgg gaagcaaccc acgaggagcg gaggccccgc
     1681 ccctttcatc gcatcaggtt gacggggctg gggtgttggg gcgtcacgag acggtgcgga
     1741 ccagcatgcc ttcgtggatg ccacgctgtc gtcgtacaaa ggtcaccggt tcccagtgga
     1801 gatcatcagc cattgtgtgt ggctgtacca ccggttcccg ctgagcctgc gcgacgtgca
     1861 ggagatgatg gccgaacgcg gtgtgatcgt ctcctacgag agcgtccatc agtggtgccg
     1921 caagttcggg caga
//
LOCUS       DATADN010000268         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_783518, whole genome shotgun sequence.
ACCESSION   DATADN010000268 DATADN010000000
VERSION     DATADN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783518"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1509
                     /locus_tag="VHH53_03580"
     CDS             247..1509
                     /locus_tag="VHH53_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289279.1"
                     /translation="MTAVTDSTVEGLDPASGPGHVAILGAGPIGLDAALTCLDKGWPC
                     TVYESAASVAANVTAWSHIRLFTPWSMNVSARMLAHLQAAGVQPPGPAEHCPTGAEFA
                     EQLLRPLAELPELAAAIRSHTRVLAVARQGLLKHEEIGSTQRGSQPFRLLLAGPRDGA
                     DDVEYVERADLVLDCTGSYGHANCLGDGGIPAPGEQQVADRIIRRIPDLDSEAEEWAG
                     RRVLLVGAGKSAQTAARDLAELVVTRPDTQVLWAVRAADPDWGEVSNDPLPQRQELVE
                     ISQRLRSGAVSGVELCTGVVVEALRAADDDVAVTLRSNNGDVEQIVVDRIIGLTGFVG
                     DTSLYRQLQVHECYATAAPMNLSAALLSAAGYGPADCLAQASYGVDTLRVPEPNFFIL
                     GMKSYGRNSTFLLRVGYEQVDEVANAYS"
     gene            complement(1532..>1954)
                     /locus_tag="VHH53_03585"
     CDS             complement(1532..>1954)
                     /locus_tag="VHH53_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289280.1"
                     /translation="RGRGSWAKAMAGLDLALSLGLPVRVGMTETPQNRDQVEPLRTLL
                     AAKGIRGRDFAVRPLVKRGYSDEGVTISEDNTVPELTVTADGWHWHPAGADLETSPDM
                     LLAGPDVSMAEAKRRTVELFLRLRQRDGSLPLVYNCAV"
BASE COUNT          315 a          661 c          647 g          331 t
ORIGIN      
        1 gtcagggcat gctgagcagc gaggtcgaag gtgccatcgg tgctgccgcc gtccaccacc
       61 accagctcgc agtcggggaa gtcgcggcgc aaccgtcgca agcagtcggc aatgcatgct
      121 gcctcattga gtgcaggcac cacgatcgac accgtcaccg gcttgctgat catgatcctg
      181 atcgatccga tcttccatga gcgtcgaata ggttgctgga aacagcccac accttacgga
      241 gtgcacgtga ccgcagtaac cgattccacc gtcgaaggcc tggaccccgc gtccggacct
      301 ggccacgttg cgatcctcgg cgccggcccg atcggtctgg atgccgcatt gacctgcctg
      361 gataagggtt ggccctgcac cgtctacgaa tcggctgctt cggtggcagc aaacgtcacc
      421 gcgtggagtc atatccggtt gttcacgccc tggtcgatga acgtgtcggc gcggatgctc
      481 gcgcatctcc aggctgctgg ggtgcagcca ccagggcccg ccgagcactg cccgaccggc
      541 gctgagttcg ccgagcagct ccttcgccca ctggccgagc tacccgagct ggccgctgcc
      601 attcgtagcc acaccagggt tctggctgtc gcccggcagg gcctgctcaa gcacgaggag
      661 atcggtagta cgcagcgcgg ctcgcagccc ttccggctgc tgctcgccgg ccccagagac
      721 ggcgccgacg acgtcgagta cgtggagcgc gccgatctgg tgctggactg caccggcagc
      781 tacggccatg ccaactgcct cggtgacggc ggcatcccgg ctccgggcga acaacaggta
      841 gcggatcgaa tcatccgccg gattcccgat ctggacagcg aagctgagga gtgggccggg
      901 cgccgggtgc tgctggtcgg cgccggcaaa tcagcccaga ccgcagcgcg ggaccttgct
      961 gagctggtcg tcacgcgacc tgacacccag gtgctctggg cggtgcgggc ggcggacccg
     1021 gactgggggg aggtctccaa cgatcccctt ccccaacgcc aggagctggt cgagatatcc
     1081 cagcgactgc ggtcgggagc ggtatccggc gtggaactat gcaccggcgt ggtggtcgaa
     1141 gccctacgtg ccgcagacga tgacgtggcg gtgacgcttc gctcgaacaa cggcgatgtc
     1201 gagcagatcg tcgtcgaccg gatcattggg ttgaccggct tcgtgggtga tacgtcgctg
     1261 taccggcagc tgcaggtgca cgagtgttac gccaccgccg caccgatgaa cctgtcggcg
     1321 gcgttgctga gcgcggccgg ttacggcccg gcggactgct tggctcaggc gtcgtacggg
     1381 gtggacactc tgcgggtacc ggagcccaac ttcttcatcc tcggtatgaa gtcctacgga
     1441 cgcaacagca ccttcctgct gcgcgtcggg tatgagcagg tcgacgaggt ggccaacgcc
     1501 tactcctagc ccggccctca tccggctccg ctcagacagc gcagttgtag accagcggta
     1561 gtgagccgtc gcgctggcga agccgtagga acagttccac ggtccgccgt ttggcttcgg
     1621 ccatgctgac gtcaggtccg gcgagcagca tgtccgggga ggtctcgagg tcagcgccgg
     1681 ccgggtgcca gtgccaaccg tcggcggtga cggtcagctc cggcacggtg ttgtcctcgc
     1741 taattgtgac cccctcatcg gagtatcccc gcttgaccag cggccgcacc gcgaaatccc
     1801 ggccccgtat tcccttggcc gccagtagcg tccgcagcgg ctcgacctgg tcgcggttct
     1861 gcggcgtctc ggtcatgccg acccgtaccg gcaggcccag cgacagcgcc aggtccagac
     1921 cggccatcgc cttggcccag gacccccgcc cccg
//
LOCUS       DATADN010000269         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1090150, whole genome shotgun sequence.
ACCESSION   DATADN010000269 DATADN010000000
VERSION     DATADN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090150"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VHH53_03590"
     CDS             <1..333
                     /locus_tag="VHH53_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2289281.1"
                     /translation="LAIGAPADGPYVLDVVRRTSAAIEDVTDAEVVEGIRLLARTEGI
                     FAETAGGVTVACAKKMVENGSLDPDAETVLLITGDGLKTLEAVQDHVGPRATVPPNTK
                     AVRAALQG"
     gene            complement(603..>2652)
                     /locus_tag="VHH53_03595"
     CDS             complement(603..>2652)
                     /locus_tag="VHH53_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEX2289282.1"
                     /translation="AVDSSGYGGRRPQPQRVVIRAELEAAELAGRQWRTGGRVVLLAP
                     AQGWTGLLPGQRVRATGLLAVPNRSDLTVAVLRVRGQPEVLAAPTAVQRVAERLRSGL
                     RVAAGVLDPKPAGLLPALVVGDTSTMVSTVEDEFRAAGLAHLTAVSGANVAILCGAVV
                     GLARLARTGPRTAAILAGLVLVGFVVLCRPSPSVLRAAAMGAVTLMALMLGRRRSAVP
                     ALCAAVLVLLLVDPALGGDPGFALSVLATGALVLLAPGWAAGLRRHGVPPGIAEALAV
                     PAAAHVVTAPVVAGLSGQVSLVAVLANLLAAPAVAPATVLGVLAAVLAPLHPATAAVV
                     VQVAGPVVGWLVGVGHHGAAVPDGVMQWPGGTSGALLLALLVSAALFAMRAHRLRVLV
                     AAGLVGALLVLVPTRYVLPGWPVTGWVVAACDVGQGDAVVLATAEPDRAVLVDTGSNP
                     GPVSACLDRLGIQRLALVVISHLHADHVGGLVGALRGRTVSAVALGPGRSPPWAFAEV
                     LRAAAVAHVPVVAVAAGQRLSWPGLVLDVLAPLGASPVVGDHDAEVDGTVVNNASVVL
                     RATTPAGRVLLTGDVELDAQADLLAARIDLRADVLKVPHHGSRYAAKEFFAAVEPRVA
                     IVSVGAHNRYGHPSQHVLDALARTGSRVARTDLNGDVLVAAGKTGLQVVMRGAVQRPP
                     "
BASE COUNT          485 a          998 c          844 g          325 t
ORIGIN      
        1 ttggccatcg gcgcgcccgc cgacggtccg tacgtattgg atgttgtccg ccgcaccagt
       61 gccgcgatcg aggatgtcac cgacgcagag gtggtcgaag gaatccgatt gctggcccgt
      121 accgaaggta ttttcgctga gaccgccggg ggggtgacgg ttgcctgcgc gaagaagatg
      181 gtcgaaaacg ggtctctgga ccccgacgcc gaaaccgtcc tgctcattac cggcgatggg
      241 ctcaagacct tggaagcagt gcaggatcac gttgggccgc gcgccaccgt gccacccaac
      301 accaaggccg tacgcgcggc acttcaaggt tgatggcgcg gtatttccgg agaataccca
      361 atagacggta acgcacggcc gcgcacacag ccagatccgg aagttctgcg gccgccatta
      421 tgcagtccgg cgacgaccca cacacccgca gcgtcggatc agcaggtcgt ctcagaccgc
      481 cgagtcacgt atggcggccc gttccacggc ctatcgagcg gcgtactcca cgtaacgaag
      541 gcgctttcgc gctgactcgg caactagcct agtcaggcac gtccgcgcga gccaccttcg
      601 ccttatggag gccgttgcac cgctccgcgc attaccacct gaagaccggt ctttcccgct
      661 gccaccaaga cgtcgccgtt gagatcggtt cgcgccactc gagatcccgt gcgtgctagc
      721 gcatcgagga cgtgctggct cggatgaccg taacggttat gtgctcccac actcacgatc
      781 gctactcgcg gctctactgc tgcgaagaac tccttggcgg cgtagcgcga gccgtggtgc
      841 gggactttga gcacgtcagc ccgcagatca atcctggcgg ccagcaagtc cgcttgagca
      901 tcgagctcca cgtctccggt cagcagcacc cgcccagcgg gggtggtcgc cctcagcacg
      961 accgaggcgt tgttcactac ggtgccatcc acctcggcgt cgtggtcgcc caccaccggc
     1021 gaggcaccca gcggagccag cacgtcgagc accaggccgg gccaggacag ccgctgtccg
     1081 gcagccaccg cgaccaccgg cacgtgagcc accgcagcgg cccgcagcac ctcggcgaac
     1141 gcccacggcg ggctacgtcc agggccgagc gcgaccgcgc tgacggtccg tccccgcagc
     1201 gcgccgacca gcccgccgac gtgatcagcg tgcaggtggc tgatcaccac cagggcgagt
     1261 cgctggatcc ccaggcggtc caggcaagcc gacaccggac ccgggttgga tccggtgtcc
     1321 accagcaccg cccgatccgg ctccgcggtg gccaagacta ccgcgtcgcc ctggccgacg
     1381 tcgcatgccg ctactaccca accggttacc ggccaacccg gcaacacata ccgcgtcggc
     1441 accagtacaa gcagggcacc gaccaatccg gccgccacga gtacccgcag ccgatgtgcc
     1501 cgcatcgcga acagcgccgc gctaacgagc aacgccaaca gcagtgcacc ggaggtccct
     1561 cctggccact gcatcacccc gtcgggcaca gcggccccat ggtgacccac gcccaccagc
     1621 caacccacca caggtccagc cacctgcacg acgacggcag cggtggcggg gtgcaacggt
     1681 gcaagcaccg cggcgagcac gccgagcacg gtcgccggtg cgaccgccgg ggcagcgagc
     1741 aggttggcca gcactgccac cagactcacc tgcccggaca ggcctgccac caccggcgcg
     1801 gtgaccacgt gggcggccgc gggcaccgcg agtgcctcgg caatccccgg cggcaccccg
     1861 tgacggcgca gcccggccgc ccagccaggc gcaagcagca ccagcgcgcc ggtggccaga
     1921 acggatagcg cgaaccccgg atcaccccct aacgccggat cgaccaggag caagaccagc
     1981 accgcggcgc acaacgcggg aaccgccgag cgacggcggc ctagcatcag tgccatcagg
     2041 gtgacggcgc ccatcgccgc ggcacgcagc acactcggag aggggcggca cagcaccacg
     2101 aagccgacca ggaccaggcc ggcgaggatg gctgcagtcc gcggtccggt gcgagccagc
     2161 cgggccagcc ccacaacggc cccacacaag atcgccacgt tggcaccgga cacggccgtg
     2221 agatgcgcta ggccagcggc gcggaattcg tcttcgactg tggacaccat tgttgaggtg
     2281 tcccccacca ccagcgcggg cagcaacccg gccggtttcg gatcgaggac tccggccgcc
     2341 acccgcagcc cggaccgcag ccgctcggca acgcgttgga ctgcggttgg cgccgccagc
     2401 acctccggct gacctcgcac ccgcagcacg gcaaccgtca ggtcagaccg gttgggtacg
     2461 gcaagcagcc cggtggcccg gacccgctgg ccgggcagca ggccagtcca gccctgcgcc
     2521 ggagccagca ggaccacccg accgccagtc cgccattgcc gcccggcgag ctccgcggcc
     2581 tcaagctcag cgcgtatgac cacccgctgg ggctgcggtc gccgaccgcc gtaacccgat
     2641 gagtcgacgg cg
//
LOCUS       DATADN010000270         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_336642, whole genome shotgun sequence.
ACCESSION   DATADN010000270 DATADN010000000
VERSION     DATADN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336642"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1206
                     /locus_tag="VHH53_03600"
     CDS             301..1206
                     /locus_tag="VHH53_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX2289283.1"
                     /translation="MPQHDRLPVRRSRFYEVGTRRHRRHFDDDEPPARQRTPAAPPPD
                     DDPIDGDRWSTWDMGQHGPAPHPDWLVTELAAVDTDLGVLKTGKEADVHLVERAVPGT
                     SRSCLLAAKRYRTTEHRMFHRDAAYIEGRRVRCSRETRAMARRTAFGRDLLAGQWAAA
                     EFSALCRLWRLGAPVPYPAQLHGTELLLEYLAEQDGQAAPRLAQLRPDPAALRTLWDQ
                     LIDALVLLTDEGLAHGDLSPFNLLVHRGRLVLIDLPQVVDIVANPQGPAFLARDIRIV
                     TDWFTAHGLPPQATRALARVAREWH"
     gene            complement(1221..>1502)
                     /locus_tag="VHH53_03605"
     CDS             complement(1221..>1502)
                     /locus_tag="VHH53_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018681978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2289284.1"
                     /translation="PDDPASLVDWAALGRLRGTLILLMAVERIGAFADVLIAHGRAPD
                     SPVAVIADGTLRSQQTVCSTLADVADVARGGIRPPAIVVIGPVVGLVSE"
BASE COUNT          243 a          535 c          494 g          230 t
ORIGIN      
        1 cggtcccgcc caccagccct cgagccatgg cgctcagaca gcgacgcagt tgtcgtagcg
       61 cggacctacg agttctgaac cctgaacgcc agtaaatcgg ttgcccacca tcggcgtgtc
      121 gggccacgct gtgcgccacg atgacggcag gtgcccgtca cgcgggccca acggtacgag
      181 aacagcggag gaggtgagag gccatggctg ccagtggagt tcagctcaac gcgacctcac
      241 actcccgcca tccagccggc tgatctgctc ctgggtggcg tcacccgagg agtccttcca
      301 atgccacagc acgaccggct gcccgtccgg cgcagccgct tttatgaggt cggcacccgc
      361 cggcatcgac gccacttcga cgatgacgaa ccaccagccc gccagcgcac tcctgctgcc
      421 ccgccacctg atgacgatcc gatcgacggg gatcgatggt cgacctggga tatgggacag
      481 cacggcccgg caccccaccc ggattggttg gtcaccgagc tcgccgcagt cgataccgac
      541 ctcggtgtgc tgaagaccgg caaagaagcc gacgtgcatc tggtggagcg cgcagtaccc
      601 ggcacgtccc gatcttgcct gctcgccgcc aagcgttacc gcaccaccga gcaccggatg
      661 tttcaccgcg acgccgccta catcgagggc cggcgggtgc gttgctcccg ggagacccgg
      721 gcgatggcgc ggcgcactgc ttttggccgc gatttgctgg ccgggcagtg ggcggctgcc
      781 gagttcagtg ccctgtgcag gctgtggcgc ctcggtgcgc cggtgcccta cccggcgcag
      841 ctgcacggga ctgagctact gctggagtac ctcgccgagc aggatggcca agcagctccc
      901 cggctggcgc agctgcggcc cgatcccgca gcgcttcgca cgttgtggga ccagctcatc
      961 gacgccctgg tgctgctcac cgacgagggt ttggcgcacg gcgacctgtc cccattcaac
     1021 ctgctggtgc accgtgggcg gctggtgctc atcgacctgc cacaggtggt ggacatcgtc
     1081 gccaacccgc agggaccggc attcctcgcg cgggacattc ggatcgtgac cgactggttc
     1141 accgcccacg ggctgccgcc gcaggcaacg cgcgccctgg cgcgggttgc tcgtgagtgg
     1201 cattgagtgc tggaacactg ttactcactc acgaggccca ccactggccc gatcacgacg
     1261 atcgcgggtg ggcggatccc gccacgagcg acatctgcga cgtcggccag cgtggaacac
     1321 accgtctgct ggctgcgcag ggtgccgtca gcgatcacgg caaccgggct gtccggtgcc
     1381 cggccatggg cgatcagtac gtcggcgaac gcaccgatcc gctcgaccgc catcagcagg
     1441 atcagggtgc cgcgcagccg cccgagcgcc gcccagtcga ccagcgaggc gggatcgtcc
     1501 gg
//
LOCUS       DATADN010000271         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1090295, whole genome shotgun sequence.
ACCESSION   DATADN010000271 DATADN010000000
VERSION     DATADN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090295"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VHH53_03610"
     CDS             <1..1512
                     /locus_tag="VHH53_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX2289285.1"
                     /translation="VQEPRAYYGELIDTYSIVGAPEGAAPVEYDTDTERYTYNGRGGV
                     PLDNIVNRFVFALFYGERNILFNSSINENSKIMYVRDPADRVQAVAPWLTMDGDPYPA
                     VVDGRITWIVDGYTTLENYPYAERTPLGESTTDALQPGQGGVRPELDRDVSYLRNSVK
                     ATVDAYDGTVTLYAFDERDPVLQTWMKTFPGTVRPAAEISPNLRSHFRYPEDQFKVQR
                     ELLTRYHVDTPGDFFNNVSFWNVPSDPSPQGTGGSSALPQPPYYILAGTPGQPGSPAS
                     FQLTSALVFQEREFLSAYLSVSSDPDTYGQMTLLRLPDNTTTQGPQLVQSALVSSPEV
                     SEQIGLLSRNGQSTVDYGNLLTLPVAGGLLFVEPVYIERANQEVSYPQLARVLVSYQG
                     RVGFDATLAGALEEVLPGSAAVVPSVPGQAQTPAPAGTPAAPTGPPGSVNPQVATAAT
                     AIQQAIADLRAANQSGDFAAQGRALAALDAAVQQFQQANGQAPAPAPAPAPGG"
     gene            1590..1666
                     /locus_tag="VHH53_03615"
     tRNA            1590..1666
                     /locus_tag="VHH53_03615"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Met,seq:cat)
     gene            1783..>1969
                     /locus_tag="VHH53_03620"
     CDS             1783..>1969
                     /locus_tag="VHH53_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289286.1"
                     /translation="MSSPAAAGSDPVAAASRFEKIQRENTELRRARRELAEHLELAIA
                     NIQRLTLDNHHLRQQLHA"
BASE COUNT          331 a          728 c          636 g          274 t
ORIGIN      
        1 gtgcaggagc cgcgcgcgta ctacggggag ctcatcgaca cgtactcgat cgtcggtgct
       61 ccggagggcg cagcgccggt ggagtacgac accgacaccg agcgctacac ctacaacggg
      121 cgcggtggcg tcccgctgga caacatcgtc aaccgcttcg tcttcgcgct cttctacggc
      181 gagcggaaca tcctgttcaa cagctcgatc aacgagaact cgaagatcat gtacgtccgt
      241 gaccccgcgg accgggtcca ggcggtcgca ccgtggctca cgatggacgg cgacccctac
      301 cccgcggtgg tggacggccg gatcacctgg atcgtcgacg gctacacgac gctcgagaac
      361 tacccgtacg ccgagcgcac cccgctcggt gagtccacca ccgacgcgct gcagcccggc
      421 cagggcgggg ttcgccccga gctcgaccgg gacgtcagct acctgcgcaa ctcggtgaag
      481 gccaccgtcg acgcctacga cggcacggtc acgctgtacg cgttcgacga gcgtgacccg
      541 gtcctccaga cgtggatgaa gaccttcccc ggcacggtgc gcccggccgc ggagatcagc
      601 ccgaacctgc gctcgcactt ccggtacccg gaggaccagt tcaaggtgca gcgcgagctg
      661 ctcacccgct accacgtcga cacgccgggc gacttcttca acaacgtctc gttctggaac
      721 gtgccgtccg acccgtcgcc ccagggcacc ggcggcagca gcgcgctgcc ccagccgccg
      781 tactacatcc tggcggggac gccgggtcag ccggggtcgc cggcgtcgtt ccagctcacc
      841 agcgcgttgg tgttccagga acgtgagttc ctgtccgcgt acctgtcggt cagctccgac
      901 cccgacacct acgggcagat gaccctgctc cgactaccgg acaacaccac gacccagggc
      961 ccgcagctgg tgcagagcgc cctggtcagc tcgcccgagg tcagcgagca gatcggtctg
     1021 ctgtcccgga acgggcagag caccgttgac tacggcaacc tgctgacact ccccgttgcg
     1081 ggcggcctgc tgttcgtcga gccggtctac atcgagaggg cgaaccagga ggtcagctat
     1141 ccgcagctgg cccgcgtgct cgtctcctac cagggcaggg tcggtttcga tgcgacgctg
     1201 gccggcgccc tcgaggaggt cctgcccggc tccgccgcgg tggtgccgag cgttcccggg
     1261 caggcgcaga cgccggcgcc tgcgggcaca cccgccgcgc ccaccggccc gccgggatcg
     1321 gtcaacccac aggtcgccac ggcagcgacc gcgatacagc aggccatcgc cgacctgcgt
     1381 gcggccaacc agagcgggga cttcgccgct cagggccggg ccctcgccgc gctggacgcg
     1441 gctgtgcagc agttccagca ggcgaacggg caggcacccg ccccggcgcc ggccccggct
     1501 cccggcggct gacgaccggc ccctgcagcg gggtaccccc cgctgcaggg gcgtttcggg
     1561 caggtgtaca gtaggagcca caacagcaac gcggggtgga gcagctcggt agctcgctgg
     1621 gctcataacc cagaggtcgt aggttcgaat cctacccccg ctaccagcac gaacggccct
     1681 cgggagatcc tcccgggggc cgttcgcatg tggtcggtag taagcattgt agtacgggag
     1741 ctggctcagc acgcctcgtc agcaggagct ggtgcctctt cggtgtcatc accagcggca
     1801 gccggctccg atcccgtcgc cgccgccagc cggttcgaga agatccaacg cgagaacacc
     1861 gaactgcgcc gcgccaggcg cgaactcgcc gagcacctcg aactggccat cgcgaacatc
     1921 cagcgactca cgctggacaa ccaccaccta cgccagcagc tccacgccg
//
LOCUS       DATADN010000272         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1090383, whole genome shotgun sequence.
ACCESSION   DATADN010000272 DATADN010000000
VERSION     DATADN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090383"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VHH53_03625"
     CDS             complement(<1..194)
                     /locus_tag="VHH53_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX2289287.1"
                     /translation="MPRKGPAPRRPLIADPVYQSPLVTQLINKVLVDGKRSVAESIVY
                     GALEGCRSKTDTDPVVTLKRA"
     gene            complement(196..384)
                     /gene="rpsL"
                     /locus_tag="VHH53_03630"
                     /pseudo
     CDS             complement(196..384)
                     /gene="rpsL"
                     /locus_tag="VHH53_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
     gene            389..529
                     /locus_tag="VHH53_03635"
                     /pseudo
     CDS             389..529
                     /locus_tag="VHH53_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078642.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
     gene            531..1001
                     /gene="rpsG"
                     /locus_tag="VHH53_03640"
     CDS             531..1001
                     /gene="rpsG"
                     /locus_tag="VHH53_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX2289288.1"
                     /translation="MPRKGPAPRRPLVADPVFNSPLVTQLINKILIGGKRQLAERIVY
                     GALEGCREKSGTDPVVTLKRAMDNVKPTLEVRSRRVGGATYQVPVEVRTPRQTTLGLR
                     WIVQYAKARREKTMIERLQNELLDASNGLGAAVKRREDTHKMAESNKAFAHYRW"
     gene            1041..>1583
                     /locus_tag="VHH53_03645"
     CDS             1041..>1583
                     /locus_tag="VHH53_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2289289.1"
                     /translation="MAAADALAKVRNIGIMAHIDAGKTTTTERILFYTGITYKIGEVH
                     EGAAVMDWMEQEQERGITITSAATKCEWKGHTIQIIDTPGHVDFTVEVERSLRVLDGA
                     VAVYDGVAGVEPQTENVWRQANKYNVPRMCFVNKLDRTGADFFRCVQMMIDRLNATPL
                     VLQIPIGLEGNHIGVVDLLGM"
BASE COUNT          293 a          518 c          533 g          239 t
ORIGIN      
        1 gcccgcttga gcgtgaccac cggatccgtg tcggtcttgg accggcaacc ctcgagggcg
       61 ccgtagacga tcgactcggc gaccgagcgc ttgccgtcga cgagcacctt gttgatgagc
      121 tgggtcacca gcggcgactg gtacaccggg tcggcgatca gcggacggcg cggtgcgggg
      181 cccttgcgcg gcatgtcagc tcttctcctt cttcgcgccg taccggctgc gagcctgctt
      241 gcggttgcgg acgccctggg tgtccagcga gccgcggatg atcttgtagc gcacgccggg
      301 gaggtccttc acgcggccgc cgcgcacgag caccatcgag tgctcctgca ggttgtggcc
      361 gacgcccggg atgtacgcgg tcacctagat ggtgctcgtg cgcggcggcc gcgtgaagga
      421 cctgcccggc gtgcgctaca agatcatccg cggctcgctg gacacccagg gcgtccgcaa
      481 ccgcaagcag gcccgcagcc gttacggtgc gaagaaggag aagagctgac atgccgcgta
      541 aaggccccgc tccacgccgg ccgctggttg cggacccggt gttcaactcc ccactggtca
      601 cgcagctgat caacaagatc ctgatcggtg gcaagcgtca gctcgccgag cgcatcgtgt
      661 acggcgccct ggagggctgc cgggagaaga gcggcaccga cccagtggtg acgctcaagc
      721 gtgcgatgga caacgtgaag ccgaccctcg aggtccgcag ccgccgtgtc ggtggcgcga
      781 cctaccaggt gccggtcgag gtccgtacgc cgcgccagac caccctcggg ctgcgctgga
      841 tcgtccagta cgccaaggcg cgccgcgaga agaccatgat cgagcgtctg cagaacgaac
      901 tgctggacgc gagcaacggg ctcggtgccg ccgtcaagcg gcgcgaggac acgcacaaga
      961 tggcggagtc caacaaggcc ttcgcccact accggtggta accgaccgcc gaacgacgac
     1021 gaagataagt agggattgca gtggctgccg cagacgcgct cgccaaggta cgcaacatcg
     1081 gcatcatggc gcacatcgac gctggtaaga ccacgacgac cgagcgcatc ctgttctaca
     1141 ccggcatcac gtacaagatc ggtgaagtcc acgagggcgc ggccgtcatg gactggatgg
     1201 agcaggaaca ggaacgcggt atcaccatca cttccgccgc caccaagtgc gagtggaagg
     1261 gtcacaccat ccagatcatc gacacgcccg gccacgtcga cttcaccgtc gaggtggagc
     1321 ggtcgctgcg cgtgctggac ggtgcggtgg cggtctacga cggtgtggcc ggcgtggagc
     1381 cgcagaccga gaacgtctgg cgtcaggcga acaagtacaa cgtcccgcgt atgtgcttcg
     1441 tcaacaagct ggaccgcacc ggtgccgact tcttccggtg cgtccagatg atgatcgacc
     1501 ggctgaacgc caccccgctg gtgctgcaga tcccgatcgg cctcgaggga aaccacatcg
     1561 gcgtcgtgga cctgctcggc atg
//
LOCUS       DATADN010000273         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_485673, whole genome shotgun sequence.
ACCESSION   DATADN010000273 DATADN010000000
VERSION     DATADN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485673"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1431)
                     /locus_tag="VHH53_03650"
     CDS             complement(10..1431)
                     /locus_tag="VHH53_03650"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX2289290.1"
                     /translation="MSIIEQLEAAGEVLSPVVRAAIQGLEALAVMVPVLQERVRELET
                     RLGLNSTNSSKPPSSDPPSVVRPAKKPTGKKRGGQKGHRGHHRKQMAADRVDVIVEHR
                     PEACGHCGHGLAGAEEAKPAWVHQVTELPPIRAQVTEHRMHSVRCPNCRKVTRAPLPA
                     EVGGKHFGPRLVGLGSLLNGRYRMSRRETVDLLGRLLDVPAPSLGSTEAFAQEASTAL
                     KPAYEQARAAVLQSDAAGVDETPWKLGGKKQWLWVATAASATVFHLGPGRGAEELKRF
                     LGEKFRGVVGSDRWCAYQIYERRQLCWAHLLRNFKKLALRGGKAVEFAEEGVKECARV
                     FALWRQLQEGQINREQLRRGIQRIRNRFRRLVRRGVESSDKRVAGFCRNLLRLQRWLW
                     TFVEEPVEPTNNAAERALRPAVLWRKGCFGNQSEAGMRYVERMLTVAATCQQQQIHPV
                     DFLTTAILALRSGTSGPTLVAVQ"
     gene            complement(1594..>1992)
                     /locus_tag="VHH53_03655"
     CDS             complement(1594..>1992)
                     /locus_tag="VHH53_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2289291.1"
                     /translation="GEADALREIDPSEFARIAYHEADADLPWMIAPETLVAATAPARA
                     YTLQDRVFALIANPDAETLTLTAIVVPAAHRGQGWGKRMLRALEAAFPDKPWQAVALI
                     PENMAPGFFEHAGWERQGISQLEMRLDLGV"
BASE COUNT          310 a          803 c          544 g          335 t
ORIGIN      
        1 cgtaaccgtt cactggaccg cgacgagcgt tgggcccgag gtgccggagc ggagcgcgag
       61 gatggccgtc gtgaggaagt cgaccgggtg gatctgctgc tgttggcacg tcgcagctac
      121 ggtgagcatg cgttcaacgt aacgcatccc tgcctcgctc tggttgccga agcagccctt
      181 tcgccacagc actgccgggc gcagcgcacg ctcggccgcg ttgttcgtcg gctccaccgg
      241 ctcctcgacg aacgtccaca gccaccgctg cagcctcagc aggttgcggc agaagcccgc
      301 cacccgcttg tcggaggact cgacaccacg ccgcaccaga cgccggaacc ggttccggat
      361 ccgctggatg ccgcgccgca actgctcccg gttgatctgc ccttcctgca actgccgcca
      421 gagcgcgaac acccgggcgc attccttcac gccctcctcc gcgaactcga ccgcctttcc
      481 accgcgcaac gccagcttct tgaagttccg cagcagatgc gcccagcaca gctgccgccg
      541 ctcgtagatc tggtacgcac accagcggtc actgccgacc acaccgcgga acttctctcc
      601 caggaaccgc ttcagttctt ccgccccgcg gcctggtccc aggtgaaaca ccgtcgcgct
      661 cgccgccgtc gcaacccaca accactgctt ctttccacct agcttccacg gcgtctcgtc
      721 cacgcccgcc gcatcgctct ggagcaccgc cgctcgcgcc tgctcgtacg ccggcttcag
      781 cgcggtgctc gcctcctgcg caaacgcctc cgtgctcccc agcgacggcg ctggcacgtc
      841 cagcaacctc cccagcaagt ccaccgtctc tcgccggctc atccgatacc ggccattcag
      901 aagactcccc agccccacca gccgcggacc gaagtgcttg ccccccacct ccgccggcag
      961 aggggcccgc gtcaccttcc tgcagttcgg gcaccgcacg ctgtgcatcc ggtgctccgt
     1021 cacctgcgcc cggattggcg gcagctcagt cacctggtgt acccaggctg gcttcgcctc
     1081 ttccgctccc gccaacccat gcccgcagtg cccacacgcc tccggccggt gctctacgat
     1141 cacatcgacc cggtccgcgg ccatctgctt gcgatgatgt ccccggtgcc ccttctgccc
     1201 acctcgcttc ttccctgtcg gcttcttcgc ggggcgcacc acgcttggcg ggtccgacga
     1261 cggcggcttc gatgagttcg tcgaattcaa cccgagccga gtttccaact cccgcacgcg
     1321 ctcctgcagc accggcacca tcactgcaag cgcttcaagg ccctggatcg ccgcacggac
     1381 cacaggcgac agaacctcac cagccgcttc caactgctct atgatgctca tgggccacaa
     1441 tatgtagtgg cccaggtaaa caaagtctac actcttatac aacccactgg acggttacgc
     1501 ggcgggttca cccgctccgt cgcgggctcc gccacaccac tccaatcttt acgctcgcga
     1561 ctccccggtt attacactaa cgcactcacg cactcacaca cccagatcca gccgcatctc
     1621 cagctggctg atgccctgcc gctcccagcc ggcgtgctcg aagaagcccg gcgccatgtt
     1681 ctccgggatc agcgcgaccg cctgccaggg cttgtccgga aacgccgcct ccagcgcgcg
     1741 cagcatccgc tttccccacc cctgcccccg gtgcgcggcc ggcaccacga tcgccgtcag
     1801 cgtcagcgtc tccgcatccg ggttggcgat cagggcgaag acgcggtcct gcagcgtgta
     1861 cgcgcgggcc ggggcggtgg cggccaccag cgtctcgggc gcgatcatcc acggcaggtc
     1921 cgcgtccgcc tcgtggtagg cgatgcgcgc gaactccgac gggtcgatct cccgcagcgc
     1981 atccgcctcc cc
//
LOCUS       DATADN010000274         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_631639, whole genome shotgun sequence.
ACCESSION   DATADN010000274 DATADN010000000
VERSION     DATADN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631639"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VHH53_03660"
     CDS             <1..503
                     /locus_tag="VHH53_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX2289292.1"
                     /translation="AAAAAAASAAAERNEAASGGATRVASRDVQTVINRAMTQLGVRY
                     SWGGGNASGPTIGIRDGGVADTFGDYRKVGFDCSGLMIYAFSRALGYSLPHYSGFQYN
                     SGRKVPLAQKRPGDMLFWATNGNIHHVALYIGGGQMIEAPYSGGAVRVTSVRYSGIMP
                     NAVRVL"
     gene            633..>1686
                     /locus_tag="VHH53_03665"
     CDS             633..>1686
                     /locus_tag="VHH53_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2289293.1"
                     /translation="MREADSGPPPPTSGPSGLLATPARDAQLIERTVFEIQRVIVGQN
                     RLVERMLVGLLARGHLLLEGVPGVAKTLAVETVARVVGGSFSRIQFTPDLVPADILGT
                     RIYRQGREQFDVELGPVVANFVLADEINRAPAKVQSALLEVMAERHVSIGGTTHPMPE
                     PFLVLATQNPIENEGVYPLPEAQRDRFLFKIQVEYPTVEEEREIIYRMGVHPPQPQRV
                     LDTGELTRLQEVAARVFVHHALVDYVVRLVLATRIPAEHGLSDVAGWVAYGASPRASL
                     GIVAAARALALIRGRDYVLPQDVVDVSADVLRHRLVLSYDALADGVPVEHIVIRVLQT
                     VPLPQVSSRPELDPAGE"
BASE COUNT          266 a          560 c          565 g          295 t
ORIGIN      
        1 ccgcggcggc ggccgcagcg gcttctgctg ccgccgagag gaatgaggct gcctctggcg
       61 gtgctacccg ggtcgcctca cgtgacgtgc agaccgtgat caaccgggcg atgacacaac
      121 tcggcgtccg ctactcatgg ggtggtggaa atgccagcgg acccacaatc ggcattcggg
      181 acggtggggt tgccgacacc ttcggcgact accgcaaggt tggcttcgac tgctcaggct
      241 tgatgatcta cgccttctcg cgggcgcttg gttactcgtt gccacactac agtggttttc
      301 agtacaactc cggccgcaag gtgccgcttg cccagaagcg tcccggcgac atgctgttct
      361 gggccaccaa tggaaacatc catcacgtgg cgctgtacat cggcggtggg cagatgatcg
      421 aggcaccgta ttccggaggt gcggtccgcg tcacctcggt gcgctacagc gggatcatgc
      481 ccaacgcagt ccgcgtgctc tgagctgatc cggccgcacg ccaccttcac agccgtcaca
      541 ctgctgcagc ggttgggttt gccctgcgtg gtgcccgaac agtgccccca aatccctggt
      601 caaaccagcc ggaccggatg cgaggaggac ccgtgaggga agctgacagc gggccgcccc
      661 cgccgacgtc cggcccatct ggcctgctgg cgaccccagc cagggacgcg cagctgatcg
      721 agcggactgt cttcgagatc caacgggtga tcgtggggca gaaccggctc gttgagcgga
      781 tgctggtggg gctgctggcc cgcgggcacc tgctgctcga gggagtgccg ggggtggcca
      841 agaccctcgc ggtggaaacc gtcgcccgag tcgtgggcgg ctcgttctcg cgcatccagt
      901 tcactcctga cctggtaccc gccgacatcc tcggtacccg catctaccgg cagggacgcg
      961 agcagttcga cgtcgagctg gggccggtgg tggccaactt cgtgctcgcc gacgagatca
     1021 accgcgcccc ggccaaggtg cagtcggcgc tgctggaggt gatggctgag cggcacgtgt
     1081 cgatcggcgg taccacccac ccgatgccgg agccgttcct cgtgctggcc acccagaacc
     1141 cgatcgagaa cgagggtgtc tacccgcttc ccgaggcgca acgtgaccgg ttcctgttca
     1201 agatccaggt ggaatacccc accgtcgagg aggagcggga gatcatctac cggatgggcg
     1261 tgcaccctcc ccagccgcaa cgggtactcg ataccggcga gctgacccgg ctgcaggagg
     1321 tcgccgcacg ggttttcgtg catcatgctc tggtcgacta cgtggtgcgg ctggtgctgg
     1381 ctacccgtat ccccgctgag cacggtctgt ccgacgtcgc cggatgggtg gcctacggcg
     1441 cgtcaccgcg agccagcctc ggcatcgtcg cagcggcgcg cgcattggcg ttgatccgag
     1501 ggcgtgacta cgtcctaccg caggatgtcg tggacgtctc ggccgacgtg ctgcgacacc
     1561 gcctggtgct gtcctacgac gcactggccg acggtgttcc ggtggagcac atcgtcatcc
     1621 gggtactcca gaccgtgccg cttccccagg tctcatctcg cccggagctg gaccctgcgg
     1681 gcgaga
//
LOCUS       DATADN010000275         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_784001, whole genome shotgun sequence.
ACCESSION   DATADN010000275 DATADN010000000
VERSION     DATADN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784001"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VHH53_03670"
     CDS             complement(<1..465)
                     /locus_tag="VHH53_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2289294.1"
                     /translation="MDYGLVLGGLAVGVIVGLTGMGGGALMTPMLVFFFGVNPLAAVS
                     SDLVTSAVMKPFGGFVHAVRRTVNWHLVGWLVLGSVPSGFAGVFLLRALGGEGGVDVV
                     VTTALGVALLVAAAALIVKAYITTTRRQWPTAGKVARVTVRPLPTIAIGVIGG"
     gene            complement(675..>1651)
                     /locus_tag="VHH53_03675"
     CDS             complement(675..>1651)
                     /locus_tag="VHH53_03675"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2289295.1"
                     /translation="VAAMTSPQQQQQMWCLILDAACARIVTHLRGRGHRPIMLKGATI
                     ATWLYPDPAQRPYRDLDVLVAPHTRRDVVAALEQIGYRSLIDELMLATSSPEEQPLVG
                     PHGVGIDLHVALKGVRLNPQRAWEILDRNSEPCDWFGFEVRALAPPARAMHLALHVAQ
                     RGLVDVKAARDLELGLTVLERPLWHAAARLAQDLQAVEAFVAGLELLPAGRALLNELV
                     LHAPISLETQLRAHSASTSAVFLERVFAAESWRQRLNIMRGRLFPSAAWLRLHRPGST
                     RTPTGMLFIRIWRPVEVLVQLPLAAWERHSHRRRAHRGLNRRHTRKRG"
BASE COUNT          290 a          580 c          550 g          231 t
ORIGIN      
        1 gccgccgatc acgccgatgg cgatcgtcgg gagcggacgg acggtgacgc gcgcaacctt
       61 gcccgcagta ggccactgcc gtcgagttgt cgtgatgtag gccttgacga tcagcgcggc
      121 ggcggcgacg agcagcgcta cacccagcgc ggtcgtgacg acgacgtcga cgccgccctc
      181 gccacccagg gcgcgaagca ggaacacccc ggcgaacccg ctcgggaccg aaccgagcac
      241 aagccagccg accagatgcc agttcaccgt acgtcgcacc gcgtggacga accccccgaa
      301 gggcttcatc accgcggagg tgaccagatc gctggacacc gcggcgaggg ggttcacccc
      361 gaagaagaac acgagcatcg gggtcatcag tgccccgccg cccattccgg tcagcccgac
      421 gatcactccg accgcgaggc cccccagtac caggccgtag tccatgcgcc cacccgtatc
      481 gtcacctccg cgtgcctggc ctgctgccag gtacggcctt gccagcaaca gactcggagt
      541 actcggcgct tccgttaccg atgcctcggc cgctcttcca cgtcagcgca cagcagggac
      601 gggtcgacga gatcggcgcc gccgcgatgc caaccaggac gcctacccgt ggatcgtgaa
      661 gaccacaggt gtggtcaacc acgcttacgt gtatgccgtc gattcaggcc gcggtgggcg
      721 cggcggcgat gactgtggcg ttcccatgcg gcgaggggta gctgcaccag tacctcgacg
      781 ggccgccaga ttcggatgaa cagcattccg gtcggcgtgc gggtgctccc cggtcggtgg
      841 agccgcagcc acgctgcgga aggaaacagc cggccgcgca tgatgttcag ccgctgccgc
      901 cagctctccg cggcgaacac ccgttccagg aacaccgcac tcgtcgaggc ggagtgagcc
      961 cgtagctgtg tctccagcga tatgggcgca tgcagcacca gctcgttcag cagggcccgc
     1021 ccggccggca gcagctccag cccggcgacg aatgcctcaa cggcctgaag atcctgcgcg
     1081 agacgggcgg cggcatgcca gagcggacgc tccagcacgg tgagacccag ctccaggtca
     1141 cgagcggcct tgacgtcgac caggccccgt tgcgcgacgt gtagcgctag gtgcatcgcc
     1201 cgagcgggcg gcgccagggc ccgcacctcg aagccgaacc agtcgcacgg ctcggagttg
     1261 cggtcgagga tctcccacgc ccgctgcggg ttcaggcgca cgcccttcaa ggccacatga
     1321 agatcgatgc ccacgccgtg cggaccgacc aggggctgct cctccggtga ggaggtcgcc
     1381 agcatcaact cgtcgatcag gctccggtac ccgatctgct ccagcgctgc cacgacatcg
     1441 cggcgcgtgt gaggtgcgac gagcacgtcg aggtccctgt aaggccgctg cgccggatcg
     1501 ggatagagcc aggtggcaat ggtggctccc ttgagcatga tcggtcgatg cccgcggccg
     1561 cgcaggtggg tgacgatcct ggcgcacgcg gcatcgagga tcaggcacca catctgttgc
     1621 tgctgctgag ggctcgtcat cgctgccaca g
//
LOCUS       DATADN010000276         3743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_486264, whole genome shotgun sequence.
ACCESSION   DATADN010000276 DATADN010000000
VERSION     DATADN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486264"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..1438)
                     /locus_tag="VHH53_03680"
     CDS             complement(338..1438)
                     /locus_tag="VHH53_03680"
                     /inference="COORDINATES: protein motif:HMM:NF010041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX2289296.1"
                     /translation="MGPRSVGVEEELLLVDPDDGRALAVASGVLHEATVHGDERAGDV
                     LTSELASQQIETNIRPCTSLDELGSELRRWRRAAAEAAESRGARVVALATSPLPVVPS
                     ITPKPRYREMIEKFGVTAEQQLTCACHIHVEVSSDEEAVAVLDRIRGWLPVLLALSGN
                     SPFWQGEDTGYASFRSQVWNRWPSAGPTELFGSAEHYHATVRAMIESDTVLDEGMIYF
                     DARLAQNFPTVEVRVADVCLHPDDAVLLAGLTRALVETAARAWNEGQPATPQRVELLR
                     LAGWRAARSGLDGSLIDAAGRPAPAQRVVRALLEHVTPALQDSGEYDVVHHLTDAVLG
                     RGNGSMLQRDVYRRGGSLADVVACAVTRSTGQ"
     gene            complement(1510..1845)
                     /locus_tag="VHH53_03685"
     CDS             complement(1510..1845)
                     /locus_tag="VHH53_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289297.1"
                     /translation="MSQEQDKAMRSDITPDQGKPALAAMRGGFGGWSPWNEEEHTKTD
                     IRECTDQHCMNVEVNCGFQGLDPDSPLSQSAGVSDDLRAAWRSKLAGEGKATTGSKSL
                     RDIVFGPTE"
     gene            2021..2410
                     /locus_tag="VHH53_03690"
     CDS             2021..2410
                     /locus_tag="VHH53_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2289298.1"
                     /translation="MMRQHTDATAGADARVDQLITLGAREVGDSCLVTVAGEIDMVTT
                     PNLRAFLQQQLEQVGSLLVIDLREVTFLGSSGLAVLVEIRETARERQLGLRLVCNSRE
                     VTRPLEATGLTELFDIHSDPDTALTTG"
     gene            2464..3024
                     /locus_tag="VHH53_03695"
     CDS             2464..3024
                     /locus_tag="VHH53_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2289299.1"
                     /translation="MAELLRTDRLLVRDWSVADAEAALKIYGHDDVSRWMYELERIPD
                     TATMRMALQSWTAEQSTMAPGTGRWAVVLLDGGALVGGITLLPMPVPEADVENGYQLA
                     PEYWGRSYITEAARALARWAFQQHSLVELFALVAPNNVRAAATARRIGMEWVGESDKY
                     HRRHLQVFRLRPDDLISAGNPRRLAP"
     gene            complement(3034..3486)
                     /locus_tag="VHH53_03700"
     CDS             complement(3034..3486)
                     /locus_tag="VHH53_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX2289300.1"
                     /translation="MKVFTGADELRAAAGEQLGTSDWMTIDQQRVNAFADATEDHQWI
                     HTDPQRAAAGPFGTTIAHGFLTLSLLPHLINQTYRVEGATMVINYGLNKVRFPAPVPV
                     GSKVQADVVLAEVTEATGGLQLVFRATLQIESGTKPGCVADWVTRVYF"
     gene            complement(3501..>3743)
                     /locus_tag="VHH53_03705"
     CDS             complement(3501..>3743)
                     /locus_tag="VHH53_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2289301.1"
                     /translation="LMRAAWFGPLVVVVRSMVRRYGVPGPALSWRLLSGPIFGNAVAT
                     LVFSDRNAELVIEKSGTGGQLEPVSQITLTDPNGQR"
BASE COUNT          683 a         1224 c         1174 g          662 t
ORIGIN      
        1 gggtgacgcc cattaccgcg aggcgggcgc cccgccgcat ggtcttcaca aaatcctccg
       61 tgtgacggtc tctggagacc gggtcggctg tgcggctcgg ggtggccgct cgccaggacc
      121 ctaagcaagc ctggtagaca tttttcctcg atagagtgaa cagaaagtga actaaccacg
      181 tttcgtaatc agaaatggat cattgctgct cgcgtaggcg gcaatattgg caaaccgcac
      241 acttcgaatc gctccctgta accgaatctc gatcgaactc ctctggcgat gcgttgcacg
      301 gccgagacca accactgtga gagcatcggg cggtcgacta ctgacccgta ctgcgagtca
      361 cggcgcaggc aacgacatcg gcaaggcttc ccccgcgccg gtacacatcg cgctgcagca
      421 tcgagccgtt gccccgcccg agcacggcgt cggtgagatg gtggacaacg tcatactcgc
      481 cggagtcctg cagcgccggt gtgacgtgct cgagcaatgc ccgaaccacg cgctgcgctg
      541 gcgccgggcg gccagctgca tcgatgagac tgccgtcgag gcccgatcgc gccgcccgcc
      601 accccgccag gcgcagcagc tcaacccgct gcggagtcgc cggctgaccc tcgttccacg
      661 cccgggctgc cgtctctacc agcgccctgg tcaagccggc cagcaggact gcgtcgtcag
      721 ggtgcaggca gacgtcggct acccgcacct ccacggtggg gaaattttgg gccagacgag
      781 catcgaagta gatcattcct tcatcgagga cggtgtcaga ctcgatcatg gcccgcaccg
      841 tggcgtggta gtgctcagcg gagccgaaca gctcggtggg cccggcagaa ggccatcggt
      901 tccatacctg cgaccggaaa cttgcgtagc cagtgtcctc accttgccag aacggtgagt
      961 tgccgctaag cgcgagcagc accggcaacc accctcggat gcggtccagc accgccacgg
     1021 cctcctcgtc ggagctcact tccacatgaa tatggcaagc gcacgtcagc tgttgctccg
     1081 cggtaacgcc gaacttctcg atcatctccc ggtatcgggg tttgggcgtg atactgggta
     1141 ccaccggcaa tggtgaagtc gctagggcga ctacccgggc cccgcgtgac tcggcggctt
     1201 cggcagcggc ccgccgccag cgccgtagct cggaccccag ctcgtcgagc gacgtgcacg
     1261 gccggatatt tgtctcgatc tgctggctag ccagctctga cgtcaacacg tcaccggccc
     1321 gctcatcacc gtggaccgtg gcctcatgca gaaccccact ggcgaccgcc agcgcccgac
     1381 catcatccgg atcaacgagc agcagctctt cttctactcc gacgcttcgt ggccccatac
     1441 cgccggatta tccaagctct tggcgatcgt gcttgtcggg gccgcgaagc agcgtagctg
     1501 cctatcctct cactctgtag gaccgaagac gatgtctctg aggctcttag atcctgtggt
     1561 cgccttaccc tcacccgcaa gcttcgacct ccaggcagcg cgcagatcgt cgctcacacc
     1621 agcggactgc gacagcggcg agtccgggtc caacccctgg aaaccgcaat ttacctcaac
     1681 gttcatgcag tgctggtcgg tgcactccct gatgtccgtt ttggtgtgct cttcttcgtt
     1741 ccacgggctc cagccaccga aaccgcctcg catcgccgcg agcgccggct tgccctggtc
     1801 cggggtgatg tcagaccgca tcgccttgtc ctgctcttgg ctcatagtgc acagtctatc
     1861 ttctcagcac cgaggtcgca tggcacagca gtgccgtacg ggcatcggca ggctcacacc
     1921 gtccttgtga ccttccgggc aagatgctta cgtggagccg ggaagcatcc gccagcccag
     1981 cgtggcgcgc cccggtccca tcacgtcaga ggaggaaata atgatgcgtc agcacaccga
     2041 tgcgacagcc ggtgccgatg cccgcgtcga ccagctgatc acactcggtg cgcgggaggt
     2101 gggggacagc tgcctggtga cggtcgcggg tgagatcgac atggtgacaa ccccgaacct
     2161 gcgtgccttc ctgcagcagc aactcgagca ggtgggttcg ctgctggtga tcgacctgag
     2221 ggaagtcacg tttctcgggt ccagtggact ggctgtgctg gttgagatcc gggaaacggc
     2281 ccgggagcgc cagctcgggc tgcggctggt gtgcaattcc cgcgaggtga ccaggccgct
     2341 agaggcaacc gggctgaccg aactgttcga catccactca gatcctgata ctgcgctcac
     2401 gaccggctga ggagcccgaa ctgaggagtt agccggcgcg gcgatccgga gtaagttcac
     2461 tccatggcgg aactgctgcg gaccgaccgg cttctggtgc gggactggtc ggtggccgac
     2521 gccgaggcag cactgaagat ctatggccac gacgatgtca gccggtggat gtatgagctg
     2581 gaacgcatcc ccgacacggc cacgatgcga atggcgctcc agagctggac cgccgaacaa
     2641 agcaccatgg caccgggaac agggcggtgg gctgtagtcc tgctcgatgg aggcgcgctc
     2701 gtcggtggga tcactctgct gccgatgcca gtacccgagg cggatgtgga gaacggttac
     2761 cagcttgcgc cggagtactg gggtcggagt tacatcaccg aggccgcccg ggcgctggcc
     2821 cgctgggcgt tccaacagca ttcgctggtg gagttgttcg cactggtcgc cccgaacaac
     2881 gtgcgggccg cggctaccgc gcggcggatc gggatggagt gggtcggcga gagcgacaag
     2941 tatcaccgca ggcacctgca ggtgttccgg ctacggcccg acgacctcat ctctgcagga
     3001 aatccacggc ggctggcgcc ctgaccgggt gcttcagaag tagacccggg tgacccagtc
     3061 cgccacgcaa cccggtttgg taccgctctc aatctgcagc gtggcgcgga acacgagttg
     3121 caggccaccg gtcgcctcgg tgacctcggc gagcacgacg tcggcctgaa ccttcgaacc
     3181 gaccggcacc ggggcgggga aacgcacctt gttcagcccg tagttgatga ccatcgtggc
     3241 gccctcgacc cggtaggtct gattgatcaa atgcggtaac agcgacaggg tgaggaagcc
     3301 atgcgcgatg gtggtgccaa acgggccggc tgcggcgcgt tgcgggtcag tatggatcca
     3361 ctgatggtcc tcggttgcat ccgcaaaggc attcacgcgc tgctggtcaa tggtcatcca
     3421 atcacttgtg cccaactgct caccggccgc ggcacgcaac tcgtcagctc cggtgaatac
     3481 cttcatcgcc gatcactctc ctaccgttgg ccgttcgggt cggtcagtgt gatctgggac
     3541 accggttcca gctggccgcc agtcccgctc ttttcgatga cgagctcggc gtttcgatcc
     3601 gaaaacacca gggtggccac cgcgttgccg aagatggggc cactgagcaa acgccaggat
     3661 agtgccggac cgggcacccc gtagcggcga accatgctgc gaaccactac aaccaatgga
     3721 ccgaaccagg cggctcgcat cag
//
LOCUS       DATADN010000277         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_179754, whole genome shotgun sequence.
ACCESSION   DATADN010000277 DATADN010000000
VERSION     DATADN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179754"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VHH53_03710"
     CDS             <1..1072
                     /locus_tag="VHH53_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX2289302.1"
                     /translation="DPEAAVTAARALRRHELFRIACADLLGLLHVDAVCAALSSVWTA
                     TLAAALDAALRAETQRRQAQPARIAVIGMGRLGGAELGYSSDADVLFVCEPSPDGVSG
                     QDAETDAVRFASSVAESVRKLLGSPSQDPPLVVDSGLRPEGRSGPLVRTLASYRTYYA
                     QWAQTWEAQALLRATPVAGDVELGERFIAEIDPVRYPAGGLTAQQATEIRRMKARVDS
                     ERLPRGADPATHTKLGRGGLADVEWTIQLLQLQHAHEVAALRTQSTSAALRAAADAGL
                     LTTSDADALLAAWQLATRTRNALTLVRGKQTDQLPSSGRELLAVARVLARSCRADPGE
                     VVDEYRRTTRRARAVVERSFGT"
     gene            complement(1084..1584)
                     /locus_tag="VHH53_03715"
     CDS             complement(1084..1584)
                     /locus_tag="VHH53_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289303.1"
                     /translation="MPSELAAQPRPRLIDLSYWLWLAACLVGVITAAATLRYFGDLQD
                     AMFSIVERQFPDEARATNAEVATATVAILIGAGVLVILVQMAMAMAMHSGRGWARFAL
                     IPAAVLGVLYSAAVFGTAPAMTRAGLLATTVLMIIAVIPMFLPGARAWFAQRRLTRSG
                     SYDFSE"
BASE COUNT          312 a          652 c          673 g          271 t
ORIGIN      
        1 ggacccggag gcggcggtga cggccgcccg cgcgctgcgc cgccatgagt tgttccggat
       61 cgcctgcgct gacctgctcg gactgcttca cgtcgacgcg gtgtgcgcgg cgctgtcgtc
      121 ggtgtggaca gctacgttgg ccgcggcgct ggacgcggca ctgcgcgccg agacgcagcg
      181 caggcaggct cagccggccc ggatcgcggt gatcgggatg ggtcggctcg gtggtgccga
      241 gctgggttac agttctgatg ccgacgtgct gttcgtctgc gagccgtcgc cggatggggt
      301 gtccggccaa gacgccgaga ccgacgcggt gcggttcgcc tcctcggtcg ccgagtcggt
      361 gcgcaagctg ctcggctcac ccagccagga cccgcccctg gtagtcgaca gcggcctgcg
      421 tccggaggga cgctcgggcc cgttggtgcg aacgctggcc tcctaccgca cgtactacgc
      481 gcagtgggcg cagacctggg aggcgcaggc gctgctgcgg gccacaccgg tggccggcga
      541 cgtagagctg ggggagcggt tcatcgcgga gatcgacccg gtgcgctacc ccgccggtgg
      601 gctgaccgct cagcaggcca ccgagatccg ccggatgaag gcccgggttg actcggagcg
      661 gctgccccgc ggcgccgacc cggccaccca caccaagctc ggccgcggag gattggctga
      721 cgtggaatgg accatccagc tgctgcagtt gcagcacgcg cacgaggtcg cggcgctgcg
      781 cacccagtcg accagtgcgg cactgcgggc cgcggccgat gcaggcctgc tcacgacgtc
      841 cgatgctgac gcgctgctgg ctgcctggca gctcgccacc cgaacccgca acgcgctgac
      901 cctggtcaga ggcaagcaaa ccgaccagct acccagctcc ggaagggagt tgctggcggt
      961 ggcgcgagtg cttgcgcgct cctgcagagc cgaccccggc gaggtcgtcg acgagtaccg
     1021 ccgcaccacc cggcgagcaa gggccgttgt ggagcgctcc ttcgggacct gaacccgcgc
     1081 ggttcattcg ctgaagtcgt agctgcctga ccgggtaagg cgtcgctggg cgaaccatgc
     1141 tcgcgcgcca ggaaggaaca tcggtatcac cgcaatgatc atcagtacgg tggtagccag
     1201 cagaccggcc ctggtcatcg ccggcgcagt gccgaacacg gcagcgctat agagcacacc
     1261 gagcaccgca gccgggatga gcgcgaagcg ggcccagccg cggcccgagt gcatcgccat
     1321 cgccatcgcc atctggacga ggatgaccag gacgccggca ccgatcaaga tcgctactgt
     1381 ggcggtggcc acctccgcgt tggtcgcgcg tgcctcgtcg gggaactgtc gctcgacgat
     1441 cgagaacatg gcgtcctgta ggtcgccgaa gtagcgcagg gtcgcagctg cggtgatcac
     1501 tcctaccaag caggccgcca gccacagcca gtaggagagg tcgatgagcc gcggtcgagg
     1561 ttgggcggca agctccgagg gcatagcccg gggtgcacgg ctcggacgtg ccggcggtgc
     1621 cgacgggggg gaatcgccgc gttctgcctg catcaggcag aaacctaccc ctggcccctg
     1681 gtgcctcacc gccgggggga gatcccctaa ccggccgtgg gggacacccc ctcagccccg
     1741 gtatgcccgt cgcagacaac ccggctgcaa ccccgatggc ggggggccgt cgcggctcta
     1801 actttgtcgt cagccggaca gaacatccgg tgagccgcac cggacgaaga ttccactcac
     1861 gatcaaggag aaacatcatg tctgacttca acaacaccac gacctacc
//
LOCUS       DATADN010000278         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_337959, whole genome shotgun sequence.
ACCESSION   DATADN010000278 DATADN010000000
VERSION     DATADN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337959"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VHH53_03720"
     CDS             complement(<1..834)
                     /locus_tag="VHH53_03720"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HEX2289304.1"
                     /translation="MATRLNAILLIPVLVGLVMGGFQVKSSIDTWQEAEDAENTARLV
                     RAALTYGNELLVERDVTAAPLLQGKGAKDPTVVEARANTDKAAEAFDKAAQNMPDRPN
                     LVRRLERFREVEPGLARLRAAAYTNQLTGVQTEEGYVETEHRLLEFANELGLGTGNIT
                     SYGRTVYAISLTKGALSLQRSIGMHMLIKPGPDGGNLASQRIALSSYAYLERIAIQEY
                     QSGGTEADIAKLTAAEKQVKAEGAKMAADAKAKDPEYVAPPSNPTTMVARLAALPSTD
                     TS"
     gene            complement(1362..>1766)
                     /locus_tag="VHH53_03725"
     CDS             complement(1362..>1766)
                     /locus_tag="VHH53_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX2289305.1"
                     /translation="HTGDKTTTTVAMDFGRITLDQDLILYLFGTPGQDRFWFMWDDLV
                     RGAIGAVVLVDTRRLADCFPAVDYFENSGLPFVIALNGFDGQQPYTPDEVREALQIGP
                     DTPIITTDARHRADAKSALITLVEHALMARLR"
BASE COUNT          259 a          566 c          624 g          317 t
ORIGIN      
        1 gctggtgtcc gtggacggca gcgcggcgag cctcgcgacc atcgtggtgg ggttggaggg
       61 cggcgccacg tactccgggt ccttggcctt ggcgtccgcg gccatcttcg cgccctcggc
      121 cttgacctgc ttctctgccg ccgtcagctt ggcgatgtcc gcttcggtgc cgccgctctg
      181 gtactcctgg atggcgatgc gctccaggta ggcgtacgag gacagggcga tgcgctggct
      241 ggccaggttg ccgccgtccg gaccaggctt gatcagcatg tgcatgccga tggagcgctg
      301 gagggagagc gcgcccttgg tcagcgagat ggcgtagacg gtacggccgt agctggtgat
      361 gttgccggtg cccaggccga gctcgttggc gaactccagc agcctgtgct cggtctcgac
      421 gtagccctct tctgtctgca cgcccgtgag ctggttggtg taggcggccg cgcgcagccg
      481 cgccagcccc ggctccacct cacggaagcg ctccaggcgc cgcaccaggt tgggccggtc
      541 cggcatgttc tgcgcggcct tgtcgaaggc ctcggccgcc ttgtcggtgt tcgcgcgggc
      601 ctctacgacc gtcgggtcct tcgcgccctt gccctgcagc aggggcgcgg cggttacgtc
      661 gcgctcgacg agcagttcgt tgccgtaggt cagcgcggcc cgcaccagac gcgcggtgtt
      721 ctccgcgtcc tcggcctcct gccaggtgtc gatcgagctc ttcacctgga agccgcccat
      781 gacgaggccg accagcacgg gtatgagcag gatcgcgttg agcctggtcg ccactcgcca
      841 gttgcggggg gagagacggc cgccgctctg ggcgggcgcg gccgatggct ccgaaccggg
      901 cacgggggcg ggcgccgctg tgcgcggcgg cggggtgaag ttgccccggg ccgacggctc
      961 gggactgttc ttgcttcgcc tcactcgacc aacaacctct cggcggtcgg caccaacgtt
     1021 gtgccgcagt ttctcagagc ccggtacgcc atcgactgac gtgtccgtca ttgactattg
     1081 ggcagttcag gcattccagc acgacgacct gcgctcttcc aaacagtccg aggtggggat
     1141 tccgagggat gtaagcccca gataaaacgg tcataaagaa cgagccccgc caaaaggcgg
     1201 ggcgattgtg cgcgcagcga gaccggatgt gcgcgacgcg tggccgtacc accagattcc
     1261 tctgccgaaa cgttatgaac accttggccg accgtgtcaa aggacacagt cggctccggt
     1321 gcatctacga caactgccgt atggcgcttc tgaattgggt gctacctcag ccgtgccatc
     1381 agagcgtgct cgaccagcgt gatcagggca ctcttcgcgt cggcccggtg ccgggcgtcc
     1441 gtcgtgatga tgggggtgtc cggaccgatc tggagagctt cccgcacctc atcgggggtg
     1501 tagggctgct ggccgtcgaa gccgttgagg gcgatgacga agggcaggcc ggagttctcg
     1561 aagtagtcga ccgcggggaa gcagtcggcg agacggcggg tgtccaccag gacgacggcg
     1621 ccgatcgcgc cgcgcaccag gtcgtcccac atgaaccaga accggtcctg gcccggcgta
     1681 ccgaagaggt acaggatcag gtcctggtcc agggtgatac ggccgaagtc catggccacc
     1741 gtggtggtgg tcttgtcccc ggtgtg
//
LOCUS       DATADN010000279         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_632270, whole genome shotgun sequence.
ACCESSION   DATADN010000279 DATADN010000000
VERSION     DATADN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632270"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..629
                     /locus_tag="VHH53_03730"
     CDS             144..629
                     /locus_tag="VHH53_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289306.1"
                     /translation="MGSSGNPYRARRHQREQQRRQHAQRTSRSEARSPKAGSSVEGRD
                     GRTAATACGWCGGPITPGRRGPIPKWCSATCRHRAWEQARAAASGLSAVELVERRIEV
                     QVPLVPTRRDWPGLLSQLASQLNDGRIYDRDLPALARALEPLLQAYRRRAHMTGAPDV
                     R"
     gene            828..1064
                     /locus_tag="VHH53_03735"
     CDS             828..1064
                     /locus_tag="VHH53_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEX2289307.1"
                     /translation="MDLEELRISRAAVVTVAQAAAVFGVDVRTVARAIENGELPALRL
                     GRRVLIPRLPLLAALGVVVDDGLTGVHNADSGDR"
BASE COUNT          244 a          509 c          547 g          251 t
ORIGIN      
        1 gcacgatcag ctgcaaacgc gcccggtggg caccccgcgc tctactggag ataccaagat
       61 cacgaggcga cacacctctt gcacccaggg tctcgggact cgttgtggcg ggttactgca
      121 ggatcgtgtg gacggtcgtc gtcatgggaa gcagcggaaa cccttaccgg gctcgacgcc
      181 atcagcggga gcagcaacgg cgccagcatg ctcagcggac gtccaggagc gaagcccgct
      241 cgccgaaggc cggctccagc gttgaggggc gagatggccg caccgcggcg acggcctgcg
      301 ggtggtgcgg cgggccgatc actccgggaa ggcggggccc gataccgaag tggtgctcgg
      361 cgacctgccg gcaccgcgcc tgggagcagg ccagggccgc tgcctccggc ctgtccgcgg
      421 tggagctcgt cgagcggcgc atcgaggtgc aggtgcctct ggtgcccacc cgccgtgact
      481 ggcccgggct gctcagccag ctcgccagtc agctcaatga tgggcggatc tacgaccgcg
      541 acctgcccgc attagctcgg gctcttgaac ctctactgca ggcgtaccgg cggcgcgcgc
      601 acatgaccgg agccccggac gtgcggtaag ccgcgttctc cggtcaggct ccagtgttcc
      661 tggagaagca ctggagaatt tctgagggcc gatttcccgc ctcgggcgca gagacgctgc
      721 cgatcgcagc cggactggag gaggccagga gacctgtcgc ccaccctcat cagtgacggc
      781 gcgttgccgc atcacggcga cctcgtcgca gaagcgtagg gagtgacgtg gacctcgagg
      841 agttgcggat aagccgagcg gccgtcgtga ctgtcgccca ggctgctgcc gtcttcggcg
      901 tagacgtgcg cacagtggcc cgcgccatcg agaacggcga gttgccggct ctccgtctcg
      961 gtcgacgggt gctgatcccc cggctgcccc tgctcgctgc cttgggcgtc gtggtcgacg
     1021 acggccttac tggcgttcac aacgccgact ctggcgaccg gtaggaacgc gaccgtcacg
     1081 actgatcggc cgcttccccg gtcgtctgca gcagcaccga gtactggggt gcttgtcgtg
     1141 gtcacatggc cctgaggcga gctgctgacg accgctaacg aggtggtccc cgacgaagtc
     1201 cccctcaggc ccggaccccg ctcgtgatac ccgcgtgata ctgccggtgg cgcattcggg
     1261 ggtagctgga tgcaaattcg gggccgttcg cattctggag ggaacacgaa ggcgcaggtc
     1321 agggcacccg taagcatcta gatgcttagc cgaggttagg agttcaagtc ccccctcgga
     1381 caccaccccg ccccggtgtt cctgtaggcc agggacctgg ggcgttgcgg cgcccacggc
     1441 cgagcctgtc gtgggtgagc cgacgtcgga cgagcggctt gaggggcagc gcctgtgtcc
     1501 tggcgccagc tgctgggtcg ccggtggcgt gcgcgttcaa gaaatcagta g
//
LOCUS       DATADN010000280         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_179962, whole genome shotgun sequence.
ACCESSION   DATADN010000280 DATADN010000000
VERSION     DATADN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179962"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VHH53_03740"
     CDS             complement(<1..430)
                     /locus_tag="VHH53_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289308.1"
                     /translation="MGRAFGITSSFDPAGPRSRLRGDQLRWNAGSAVSRYLRLTPWQS
                     TLPFKSVGVAEEDAVDRAEVVDGAITGCLIDQPLPVQVNRQSLSAPHLPIAPLGDRPA
                     DVHTLLPAADRRDRQVRGAQGLTVDLNRQRLIDKAASDGAI"
     gene            complement(456..1373)
                     /locus_tag="VHH53_03745"
     CDS             complement(456..1373)
                     /locus_tag="VHH53_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2289309.1"
                     /translation="MGGALNGKVALVAGATRGAGRGIAVQLGAAGATVYATGRTTRAQ
                     RSEMDRPETIEETAELVTQAGGEGVAVQVDHLEPAQVRALVERIDREQGRLDVLVNDV
                     WGGDPLAEWNKPVWEHSLDKGLRLLRLAVDTHIITSHCALPLLIRQQGGLVVEITDGT
                     AEYNTNYRGTVGLYYHLAKISAIHLALVQADELGPHGATALSLTPGWLRSEMMLEGFG
                     VTEDNWRDATAKEPHFGISESPHYVGRAVAALAADPELARWNGKSLSSGYLAQVYGFT
                     DLDGSQPNAWRYIVEVERAGKPADTTGYR"
BASE COUNT          335 a          624 c          576 g          352 t
ORIGIN      
        1 tgatggcgcc atcactggct gccttatcga tcagccgttg cctgttcagg tcaaccgtca
       61 atccctgagc gcccctcacc tgccgatcgc gccggtctgc ggccggaagt agcgtgtgga
      121 cgtccgcggg ccgatcccct aacggcgcga tcggcaggtg cggggcgctc agggattgac
      181 ggttgacctg aacaggcaac ggctgatcga taaggcagcc agtgatggcg ccatcaacga
      241 cttcggcgcg gtccacagca tcttcttccg cgaccccgac ggacttgaag ggaagtgttg
      301 attgccaagg cgtgagcctg aggtaccgac tcactgcaga gccggcattc cagcgcagct
      361 gatcaccacg caatcggctg cgcggaccgg cggggtcgaa tgagctggtg ataccgaatg
      421 ccctgcccaa gcgggatccc tgaccgagat tccgatcatc ggtacccggt ggtgtcggcg
      481 ggcttgccag cgcgttctac ttcgacgatg tagcgccagg cgttgggctg actgccgtca
      541 agatcggtga agccgtacac ctgggcaagg tagccgctgg acagcgactt cccattccag
      601 cgggctagct cgggatctgc ggcgagggcg gcgacggcgc ggcctacgta gtgcggcgat
      661 tcggagatgc caaagtgtgg ttccttcgcg gtggcgtcgc gccagttgtc ctcggtaacc
      721 ccgaaaccct ctagcatcat ctccgatcgc agccaacccg gtgttagaga tagtgcagtg
      781 gcgccatgcg ggccgagctc atcggcctgg accagggcta ggtgaatggc ggagattttg
      841 gcgaggtggt agtacaggcc gaccgtgccc cgatagttgg tgttgtactc ggctgtgccg
      901 tcggtgatct ccacgaccag cccgccctgc tgcctaatca gtagcggcag cgcgcagtgg
      961 ctcgtgatga tgtgcgtatc gaccgctagc cgcagcagcc gcaatccctt gtccagggag
     1021 tgttcccaca cgggcttgtt ccattcggcg agcggatcgc cgccccacac gtcgttgacc
     1081 agcacgtcga gccgaccctg ttcgcggtcg atccgctcca ccagcgcccg cacctgcgca
     1141 ggttccaggt gatcgacttg gactgcgaca ccctcgcctc cagcctgagt gacaagctcg
     1201 gcggtctcct cgatcgtctc cggacggtcc atttccgaac gctgcgcccg cgtggtgcgt
     1261 cctgtcgcgt agaccgttgc ccccgctgcg ccgagctgta ccgcgatccc tcgccccgct
     1321 ccgcgagtgg cgcccgcgac cagtgcaacc ttgccattca gcgctccacc catctctgct
     1381 gtgcctcccg tccaagtaac tgccgccgag tcctgccatc gggttggact gccatcgagc
     1441 tttgcgcaga gcaccgacag ttcgtccgct cgtcgagaac catgaccgca tcatcggtcg
     1501 gttctgacgc gaagtggcgt ttgatagcaa catcaggcca ccttgtgcag tgtcggtagc
     1561 ggaaccacta ccggtaacgc cgcaggtcag tcccggtgcc gctcaagggg tcccgtaagc
     1621 cccgccgatg aagattccca gaagagggca ttcctcccgc gcaacccacc aggaacgatc
     1681 ttgttatgcc acaatccgta cagcagcttt gactctcatg acccacagtt cgagtttcgc
     1741 accaccgggc gcaccgcaaa cgggcagctc agggcagact tatggctccg gcggggcccc
     1801 agcaggattc gaacctgcga cacacggttt acgaaaagct gcgacccggc ccataggctg
     1861 attaccagcg actacagttc gcgaagc
//
LOCUS       DATADN010000281         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_941215, whole genome shotgun sequence.
ACCESSION   DATADN010000281 DATADN010000000
VERSION     DATADN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941215"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VHH53_03750"
     CDS             <1..338
                     /locus_tag="VHH53_03750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289310.1"
                     /translation="PGPQLWQLVARRRVFLHTARWTSLGLSLLEAMFLGMPVVAVAST
                     MAPLVVPAEAGVVSADVETLASALERFVSDPPAAGAAGKAARDFAMAHFSLDRFLGDW
                     DRLIDDCCK"
     gene            359..1594
                     /locus_tag="VHH53_03755"
     CDS             359..1594
                     /locus_tag="VHH53_03755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289311.1"
                     /translation="MKIAMVSEHASPLAALGGVDAGGQNVHVAELSAAMARRGHDVTV
                     YTRRDDPDLPERVTTPQGYTVVHVPAGPARQLPKDELLVHMGPFAQYLDQQWSADRPN
                     VAHAHFWMSGIATQLAARHLDLPAVQTFHALGVVKRRHQGAQDTSPEDRLKLEAMVAR
                     TATWVAATCTDEVFELMRLGRSRNRISVVPCGVDLDLFTPDGPRAGRSARHRIVSVGR
                     FVPRKGFDVVVRALPAIPDAELVLVGGPDRSEVEADPEARRLRELAERLNVSDRLIFQ
                     GAVARADMPALLRSADVVACTPWYEPFGIVPLEAMACGVPVVAAAVGGMLDTVVHDVT
                     GRLVTPKRPGEVADAINTLLHDEFLRQSLGAAGRDRARARYSWDRVAADTLRIYDRLV
                     PAAYQPHESTTSATTASSV"
BASE COUNT          247 a          604 c          640 g          281 t
ORIGIN      
        1 tcccgggacc gcaactgtgg cagctggtgg cgcgacggcg ggtgttcctg cacaccgcgc
       61 gctggacctc gctggggctg tccctgctgg aggcgatgtt cctggggatg ccggtggtgg
      121 ctgtcgcctc cacgatggcc cccttggtgg tgcccgccga ggcgggtgtg gtgagtgccg
      181 atgtcgagac gctggcatcg gccctggaga gattcgtgtc cgatccgccc gcggccggcg
      241 ccgcgggtaa ggcggcgcgc gatttcgcga tggcgcattt ctcgctcgac cggttcctcg
      301 gtgattggga ccggttgatc gacgactgct gcaaatgacg tcgcgggaaa ggaagccgat
      361 gaagatcgcg atggtttctg agcatgccag cccgttggcg gcactcggtg gggtggatgc
      421 cggcggtcag aacgtccatg tggccgaatt gtcggccgcg atggcgcggc gcggccacga
      481 cgtcaccgtc tacacccgcc gcgatgatcc ggatctgccc gaacgggtca ccacgccaca
      541 gggttacacc gtggtccacg tgcccgccgg tccggcgcgt caactgccca aagacgaact
      601 cctggtccac atgggcccgt tcgcgcagta cctcgaccag cagtggagtg ccgaccggcc
      661 gaacgtggcc cacgcgcact tctggatgtc gggtatcgcc acccagttgg ccgcccggca
      721 tctcgatctg cctgcggtac agactttcca cgccctcggg gtggtcaaac gccgccacca
      781 gggcgcccag gacaccagcc ccgaggaccg gctcaaactg gaggcgatgg tggcccgcac
      841 cgccacctgg gtggcggcca cgtgcaccga cgaggtgttc gagctgatgc ggctcggccg
      901 gtcgcgcaac cggatttcgg tggtgccgtg cggggtcgac ctcgacctgt tcacaccgga
      961 cggcccgcgt gccggacgca gcgcgcggca ccgcatcgtc agcgtcggaa ggttcgtgcc
     1021 gcgtaagggt ttcgacgtgg tggtccgtgc gctgcctgcg atcccggacg ccgaactcgt
     1081 cctggtcggc ggacccgacc gcagcgaggt ggaggcggat cctgaggccc gccggctgcg
     1141 tgaactcgcc gagcggctca acgtcagtga ccggttgatc ttccagggtg cggtggcgcg
     1201 ggcggacatg cccgccctgc tgcggtcggc cgatgtggtc gcctgcaccc cgtggtacga
     1261 accgttcggc atcgttccgc tggaggcgat ggcgtgcggc gtgcccgtgg tggccgccgc
     1321 ggtgggcggc atgctcgaca ccgtcgtcca cgacgtcacc gggcggctgg tcaccccgaa
     1381 gcgcccgggg gaggtcgccg acgccatcaa caccctgctg cacgacgaat tcctcaggca
     1441 gagcctgggt gccgcgggac gggaccgtgc cagggcgcgg tattcctggg accgcgtcgc
     1501 cgccgacacc cttcgcatct acgaccggct ggtgcccgcg gcctatcaac cgcacgaatc
     1561 cacgacgtcc gcgacgaccg cgtcatcggt atgagggcgt catcgggact ccagcagctc
     1621 gagcgcccgc tcgatccggt cgggcagacg acggcgtccg cgcaccacgg cgggcaggcg
     1681 caccacggct tcggttgcgg ccctggcatg ttcgacatcg cgcagcgtcg cggtgagcag
     1741 cgtgccggtg gcccggaggc agtgcccggc gg
//
LOCUS       DATADN010000282         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1391569, whole genome shotgun sequence.
ACCESSION   DATADN010000282 DATADN010000000
VERSION     DATADN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391569"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /gene="rho"
                     /locus_tag="VHH53_03760"
     CDS             <1..878
                     /gene="rho"
                     /locus_tag="VHH53_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX2289312.1"
                     /translation="FMKLTPLYPQDRLRLETEPHLLTSRVIDLVMPIGKGQRALIVSP
                     PKAGKTMILQALANAITRNNPECHLMVVLVDERPEEVTDMQRSVKGEVIASTFDRPPS
                     DHTSLSELAIERAKRLVEMGHDVVVLLDSITRLGRAYNLAAPASGRILSGGVDSTALY
                     PPKRFLGAARNIEDGGSLTIFETALVETGSTMDTVIFEEFKGTGNAELKLDRKIADKR
                     IFPAIDVNPSGTRKEELLLSPDELAIHHKLRRVLSGLETQQAIDVLLDRLRKTRTNIE
                     FLMQVSKSAAGNGDD"
     gene            1117..1329
                     /gene="rpmE"
                     /locus_tag="VHH53_03765"
     CDS             1117..1329
                     /gene="rpmE"
                     /locus_tag="VHH53_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX2289313.1"
                     /translation="MKQGIHPQYVDTQVTCSCGKAFGTRSTKRDGQIHVEVCSNCHPF
                     YTGKQKILDTGGRVARFEKRYGKRQK"
     gene            1384..>1881
                     /locus_tag="VHH53_03770"
     CDS             1384..>1881
                     /locus_tag="VHH53_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEX2289314.1"
                     /translation="MLDQHADLERRLADPALHADQDAARKAGRRYGELSRIVRVIREL
                     EQTRSDVAAGRELAAEDPAFAAEADTLAERVPQLEAELAELLLPRDPHDSSDVVMEIK
                     SGEGGAESALFAGDLLRMYLRYAERHGWQAEVLDATEAELGGYKDVTVAVKSATSAQS
                     GVWERL"
BASE COUNT          348 a          604 c          623 g          306 t
ORIGIN      
        1 agttcatgaa gctgaccccg ctctacccgc aggaccggct ccgcctggag accgagccgc
       61 acctgctcac ctcgcgggtg atcgacctgg tcatgccgat cggcaagggc cagcgggcgc
      121 tcatcgtgtc gccgcccaag gcaggcaaga cgatgatcct gcaggcactc gccaacgcca
      181 tcacccgcaa caatccggaa tgccacctca tggttgtgct cgtcgacgag cggcccgagg
      241 aggtcaccga catgcaacgc tcggtgaagg gcgaggtcat cgcctccacc ttcgatcggc
      301 cgccgtcgga ccacacctcc ctctcggagc tggccatcga gcgagccaag cggctggtgg
      361 agatgggcca tgacgtggtt gtgctgctgg actcgatcac gcggctgggc cgggcataca
      421 acttggcagc accggcatcc ggccggatcc tgtccggtgg tgtggactcc accgcgctgt
      481 acccgccgaa gcggttcctc ggggctgccc gcaacatcga ggatggtgga tcgctgacca
      541 tcttcgagac cgcgctggtg gagaccggtt cgacgatgga cacggtgatc ttcgaggagt
      601 tcaagggaac cgggaacgcc gagctcaagc tcgaccgcaa gatcgctgac aagcggatct
      661 tccctgccat cgacgtcaac ccgtccggta cccgtaaaga ggagctgctg ctgtccccgg
      721 acgaactggc catccaccac aagctgcggc gggtgctgtc cgggttggag acccagcagg
      781 ccatcgatgt gctgctggac cggctgcgca agacccgaac caacatcgag ttcctgatgc
      841 aggtatccaa gtccgcagct ggcaacggcg acgactgacc agttctcacc gcacgcatac
      901 cgcacgcatg tcgcgctgtc atgtccggtg tgttgtgctg gggtgacctg tgctggtcgc
      961 ccctgtaagc gggaacactg cagtgcagct gacggtttca tctggggctg ggccgtctgg
     1021 cacactgtca gcgctcgctg cggcccatgc agttcggttc cggttcactc cgtccatgcg
     1081 cggcggggac ccggcggcca tgaggagagg acatccatga agcagggcat ccacccgcag
     1141 tacgtcgaca cgcaggtcac ctgcagctgt ggcaaggcct tcggcacccg cagcaccaag
     1201 cgtgacggtc aaatccatgt cgaggtgtgc tccaactgcc accccttcta caccggcaag
     1261 cagaagatcc tggacaccgg tggccgggtg gcccgcttcg agaagcgcta cggcaaacga
     1321 cagaagtaag gcccagatca caggaaatcg gtgagggcgg tggataccgc ggcagtttag
     1381 gcgctgctgg accagcacgc cgacctggag cgccggcttg ctgatcccgc gctgcatgcc
     1441 gaccaggacg cggcgcgcaa ggcgggacgg cgctatggag agctgtcccg catcgtgcgg
     1501 gtgattcgtg agctggagca gacccgcagt gacgtcgcgg cgggccggga actagctgct
     1561 gaggaccccg cctttgccgc cgaggctgac accctcgcgg agcgggtccc gcagctggag
     1621 gcagagctgg ctgagttgtt gctgccgcgc gatccccacg acagctccga cgtggtcatg
     1681 gagatcaagt caggagaagg cggcgcggag tccgcgctgt tcgccggaga cctgttgcgg
     1741 atgtatctgc gctacgccga acgccacggc tggcaggccg aagtgctcga cgctaccgag
     1801 gccgagctcg gtggttacaa ggacgtcacg gtcgcggtga aatcagcaac atcggcacag
     1861 tccggtgtct gggaacggtt g
//
LOCUS       DATADN010000283         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1391679, whole genome shotgun sequence.
ACCESSION   DATADN010000283 DATADN010000000
VERSION     DATADN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391679"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VHH53_03775"
     CDS             complement(<1..694)
                     /locus_tag="VHH53_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289315.1"
                     /translation="MAVVEPAPSFTLQPEIDEAESKAGTRSRRWRSQLGTWIPLGIII
                     FVVVACVLGPWLLGLPRPTGGSVLESNLPAGSPGHILGTDPNGNDILSRLLHGGRSSL
                     WIATAVNIIGLVLGGTLGALSGYIGGVLDTIIMRVLDVLIAFPSLVLTLAVAQSLGPS
                     RINTILALAFFSVPAFARVSRAATLRLRELPFMAAAQLCGTPGWRVLLRHIAPNITPQ
                     LIMFGMLGMGIVM"
     gene            complement(681..1721)
                     /locus_tag="VHH53_03780"
     CDS             complement(681..1721)
                     /locus_tag="VHH53_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289316.1"
                     /translation="MTATATTPRVPANSRGPRASRVSRLTSSPVLRLVVKRLLMAIPI
                     MLGVTILTFWVLSLIPGNAAQQLLGPEATPEQVAQLEEQLGLNRPGYVRYWEWLQGAV
                     TGDLGNSLVSRQPVDALIADRLAVTGELVFLAFVVSLVLAIPVALLAAHKPDGVMDRI
                     SMVISTLGLSVANYVLALILVLVFAVNLGWLPAIGYVPLSEGLWANLEGMILPSAAIG
                     LPLFCFYTRFLRGDLVDQMQQEEYVTTARAKGVGSWAVLIKHAFRNSSFGLITVVGLN
                     LGALIGGTVIVEQIFALPGLGQTLLQAINTRDFIVVQAAVVIFALVAVIANLVVDLLY
                     AVLDPRIRYGSR"
     gene            complement(1718..>1935)
                     /locus_tag="VHH53_03785"
     CDS             complement(1718..>1935)
                     /locus_tag="VHH53_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2289317.1"
                     /translation="DAAATLDEDERADLYQQAAELIAENFYAPFGLAFAPANIAVQGV
                     HGPGLTTKIPPILVNPGVIWDEVWQEQ"
BASE COUNT          327 a          667 c          694 g          247 t
ORIGIN      
        1 ccatgacgat gcccatgccc agcatcccga acatgatcag ctgcggggtg atgttcgggg
       61 cgatgtggcg cagcagcacc cgccaccccg gcgtgccgca cagctgcgcc gcggccatga
      121 acggcagttc ccgcaaccgc agcgtcgccg cccgcgacac ccgggcgaac gccgggacgc
      181 tgaagaacgc cagcgccagg atcgtgttga tccggctcgg tccgaggctc tgcgcgacgg
      241 cgagggtgag caccagcgac gggaacgcga tcagcacgtc cagcacccgc atgatgatcg
      301 tgtcgaggac cccgccgatg tagcccgaca gcgcgcccag ggtcccgccg aggaccaggc
      361 cgatgatgtt gaccgccgtg gcgatccaca gcgacgagcg gccgccgtgc agcagccggg
      421 acaggatgtc gttgccgttc gggtcggtgc ccaggatgtg cccgggggac ccggccggca
      481 ggttgctctc caggacgctg ccgccggtgg gccggggcag gccgagcagc cacggcccca
      541 gcacgcaggc gaccacgacg aagatgatga tcccgagcgg gatccaggtg cccagctgcg
      601 agcgccaccg gcggctgcgc gtgccggcct tcgactcggc ctcgtcgatc tccggctgga
      661 gggtgaagga cggggccggc tcaacgactg ccatagcgga tcctcgggtc aaggacggcg
      721 tagagcaggt cgaccaccag gttcgcgatc accgcgacga gcgcgaagat cacgacggcg
      781 gcctgcacca cgatgaagtc acgggtgttg atggcctgca gaagggtctg ccccaggccg
      841 ggcagggcga agatctgctc gacgatgacc gtgccgccga tcagcgcgcc caggttgagg
      901 ccgaccacgg tgatcaggcc gaaggacgag ttccggaacg cgtgcttgat gagcaccgcc
      961 caggacccga cgcccttggc gcgcgccgtg gtgacgtact cctcctgctg catctggtcg
     1021 accaggtcgc cgcgcaggaa gcgggtgtag aagcagaaca gcggcaggcc gatcgccgcc
     1081 gagggcagga tcatcccctc gaggttggcc cacaggccct ccgacagggg gacgtacccg
     1141 atggccggca accagccgag gttcaccgcg aagacgagca ccaggatcag cgccagcacg
     1201 tagttggcca ccgacagacc cagcgtgctg atgaccatgc tgatccggtc catgacccca
     1261 tcgggcttgt gcgccgccag cagcgcgacc gggatggcca gcaccagcga gacgacgaag
     1321 gccaggaaga ccagctcccc ggtcaccgcc agccggtccg cgatgagggc gtccacgggc
     1381 tgccggctga ccagcgagtt gcccaggtcg ccggtgacgg cgccctgcag ccactcccag
     1441 taccggacgt agccgggccg gttgaggccg agctgctcct cgagctgggc gacctgctcg
     1501 ggcgtggcct ccgggccgag cagctgctgc gccgcgttgc ccgggatcag gctgagcacc
     1561 cagaacgtca ggatggtgac cccgagcatg atcgggatcg ccatcagcag ccgcttgacg
     1621 accagccgga ggaccggcga gctcgtcagg cgggatacgc gggaggcccg cgggccccgg
     1681 ctgttcgccg ggacccgcgg ggtggtcgcc gtggcggtca ctgctcctgc cagacctcgt
     1741 cccagatgac cccggggttg accaggatcg gcgggatctt ggtggtcagc cccggaccgt
     1801 gcacgccctg gacggcgatg ttggccggtg cgaacgccag accgaacggc gcgtagaagt
     1861 tctccgcgat gagctcggcg gcctgctggt acaggtccgc gcgctcgtcc tcgtcgaggg
     1921 tcgccgccgc gtcgg
//
LOCUS       DATADN010000284         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_941566, whole genome shotgun sequence.
ACCESSION   DATADN010000284 DATADN010000000
VERSION     DATADN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941566"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..670)
                     /locus_tag="VHH53_03790"
     CDS             complement(185..670)
                     /locus_tag="VHH53_03790"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2289318.1"
                     /translation="MSDLPCVEVSLTVAAPPERVWSLVSDVTRVGEWGGECVAAHWEE
                     QGGPVVGARFRGKQVRGDRTWETSSVVTEAQPGVSFGWAVEDPANAAATWRYELAPDG
                     SGRTVVRYRAVMGPGPSGLTAVVARRPELEERFIAERLKEHERNMMATLEAIKRVAEQ
                     G"
     gene            742..>2228
                     /locus_tag="VHH53_03795"
     CDS             742..>2228
                     /locus_tag="VHH53_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289319.1"
                     /translation="MLVDQERRSSDGLARRCRRARACLAVTVALHVSTLALTVALLSL
                     GHTGQADSLVIILALAAGPPAGMLWLQRSRPSAHKPHQWPWRALAVAVPQAMALGFAL
                     NALLDEDALTPPQRILLVLVVALVPGVTAELARRSLLRPPVAELGADDFELLVEPRTG
                     QRSWFYSDSVALTDREIVITVHSGSGRATRSPKIEQIALVDVTAVGARPALPSDSPWI
                     TLPDGRGLPVAPGDVVVIQCRDTVSLLPVDDAAVFAEVVRARATRVREAPVGTLPVEV
                     DVPPAEPGHVPEVLPAPEPSGPVAHPVTGPAAPPRTGPGMALRWGLGFPLALLGIVGA
                     PVGLLLSGGLLPPETDLERYRLVFCGLWTALAAGIWLRSWQQPRYWPVWALTSGLATA
                     IIVAVQGLGLAALLGPIAGVLLTWSGRWMLIRPVGTDLARSAVEIPLRLRGGETLLVQ
                     RDRVIVKVRGGAGGAVPQALALGELAFAQLGQFACGEARFWPLPGA"
BASE COUNT          278 a          769 c          816 g          365 t
ORIGIN      
        1 caagaccctc ggccgctggt tcctgcgaag gtacgggccc aagctgcgag cgcttgcgat
       61 cggcagagca gcacctttcg gtatcggagc ggctatcggg gctgccggca acgcgcgctc
      121 gggcaacagg tggtggacac cagccagcgc gtgttcggcc cggcaccgtc gagttggccg
      181 cgggtcagcc ctgctccgcc acccgcttga tcgcctcgag ggtcgccatc atgttccgtt
      241 catgctcctt caggcgctcg gcgatgaagc gttcctcgag ctcaggcctc ctggcaacca
      301 ccgcggtcag gccggagggg ccgggaccca tcacggcccg gtagcgcacg actgtgcggc
      361 ccgacccgtc cggagccagc tcgtagcgcc aggtggctgc ggcgttcgcc ggatcctcca
      421 ccgcccaccc gaagctgacg ccgggttgcg cttcggtcac caccgaggag gtctcccagg
      481 tgcggtcgcc gcgcacctgc ttgccgcgga agcgggcgcc gactaccggt ccgccttgtt
      541 cctcccagtg agcagccacg cattcgccac cccactcacc gacccgggtg acgtcactga
      601 cgagtgacca gacccgttcc ggcggggcag ccaccgtgag ggagacctcg acacaaggga
      661 ggtcagacat acgcactcgc gcatgatatc cgtccggtgt cactgtgaga ggctccgcgc
      721 agccaacggt aggggttcgc ggtgctggta gaccaggaac ggaggagctc ggacgggctg
      781 gcgcgccggt gccggcgggc tcgggcatgt ttagcggtca ccgtcgcgct gcacgtatcg
      841 acactcgcgc tcacggtcgc gctgctctcg ctggggcata ccgggcaagc cgattccctg
      901 gtgatcatcc ttgcgctcgc ggcgggaccg ccagccggaa tgctttggct gcagcgcagc
      961 cgcccgagcg cgcacaagcc gcaccagtgg ccgtggcggg cgctggcggt ggcggttccg
     1021 caggccatgg cgctggggtt cgcgctgaat gcactgctgg atgaggatgc gctgacgccg
     1081 ccgcaaagga tcctgctcgt cctcgtcgtc gcgttggtcc caggcgtgac cgcggaactg
     1141 gcccgccggt cgctgctgcg gccgccggtg gcggagctgg gcgcggatga cttcgagctg
     1201 ctcgtcgagc cgcgcaccgg tcagcgatcc tggttttaca gtgacagcgt cgcgctgacc
     1261 gaccgggaga ttgtcatcac ggtccactcc ggctccggcc gggcgacccg gagtccgaag
     1321 atcgagcaga tcgcacttgt ggacgtcacc gctgtcgggg cccgcccggc gttgccgtcg
     1381 gatagcccgt ggatcacgct gcccgacggc cgtggactgc ccgtggcgcc cggtgacgtc
     1441 gtcgtaatcc agtgccgcga caccgtttcg ctgctaccgg tcgacgacgc agcggtgttc
     1501 gccgaggtgg tccgcgcccg ggcaaccagg gtgcgggagg ctccggttgg caccttgccc
     1561 gtcgaggtcg atgtgccacc cgccgagccg gggcacgttc cggaggtgct gccggcaccg
     1621 gaaccatccg gtcccgtcgc acacccggtc accggtcccg ctgcgccgcc gcgcaccggt
     1681 ccggggatgg cgctgcggtg ggggctgggg ttcccgctcg cgctgctggg gatcgtcggc
     1741 gcgccggtgg ggctgctgct gtccggtggc ctgctgccgc cggaaactga cctggagcgc
     1801 taccgcctgg tgttctgcgg attgtggacc gcgctggccg ccgggatctg gctgcggtcg
     1861 tggcagcagc cgcgctactg gccggtctgg gcgctgacgt ccggactcgc caccgcgatc
     1921 atcgtcgcgg tgcagggcct cgggttggcg gccctgctcg ggccgatcgc cggcgtgctg
     1981 ctcacctggt ccgggcgttg gatgctgatc aggccggtcg gcaccgatct cgccaggtcg
     2041 gcggtggaga tcccgctgcg gctgcgcggt ggggaaacgc tgctcgtgca gcgcgaccgg
     2101 gtgatcgtca aggtgcgcgg cggcgcgggt ggggcggtgc cccaagccct cgcgttggga
     2161 gagttggcgt ttgctcagct cggccagttc gcctgcggcg aggcgcggtt ctggccattg
     2221 cccggcgc
//
LOCUS       DATADN010000285         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_941698, whole genome shotgun sequence.
ACCESSION   DATADN010000285 DATADN010000000
VERSION     DATADN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941698"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VHH53_03800"
     CDS             <1..1629
                     /locus_tag="VHH53_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX2289320.1"
                     /translation="TPGWAVLGVLVALAVTGPLLALPASFLFGGEGFDQIALALLPPA
                     LTKSVVLGLGVAVGTLVIGGGLAVLISFYDFPGRRWLDWALMLPLAMPGYVLVFVLVG
                     QFGYANPLQSSLFGSGLQLPGLRGPAGAIGVLTAVLYPYVYALGRSAFLGQSRQSMEV
                     ARNLGRSYGQAVRDVALPLARPALAAGAALAVMEALADFGVVNLLGYQALTDAIYRVW
                     YGAFDQAAALQLATVLVALALIMVALERLLRGRARYHQTLARGDAVTPRRLRGWRAGL
                     ATALPCLLLAVVFAVPVLQLLAWSIETVLADRVSAGLGQAAFNTLLLAMAAAVITVIT
                     STVVVYGQRVAPSRIGAVAARLAAVGYAVPGTVIAVAIYVPLVWIDRRLIRGVEGLFG
                     TSIGLLFTGTIIGLVCAYVVRFHAQAFFALESRMGRISPNLDDAARSLGAGRARVLTK
                     VHLPLLWPGVLTAALLVMVEVMKELPATALLRPLGGDTLAIMAWEATKDARFDTAALP
                     ALLIVAVGLLPVVVLIRLWSQSGPSSATDPVTPG"
     gene            complement(1568..>2064)
                     /locus_tag="VHH53_03805"
     CDS             complement(1568..>2064)
                     /locus_tag="VHH53_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289321.1"
                     /translation="RTCLGLEYFVFSGDELWELPDADLVELGTRELAALGLVDAEQVE
                     AGYVVRMPKAYPVYDHDFQTNVAVLRNWLAIHARNVHPVGRNGMHRYNNQDHSMLTAM
                     LTVENILGGSDHDIWSVNVEGDYHEENTVAGTGRDAPILPRRAIPVSRDQSPRTDQIG
                     STGG"
BASE COUNT          253 a          697 c          725 g          389 t
ORIGIN      
        1 acgccgggat gggcggtcct gggcgtgctg gtcgcgcttg ccgtgaccgg accgctcctc
       61 gcgctacccg ccagcttcct gtttggcggc gagggcttcg atcagatcgc cctggcgctg
      121 ctgccgccgg cgctgaccaa aagcgtggtg ctcggcctcg gtgtcgccgt cgggacgctc
      181 gtgatcggtg gtgggctggc cgtactcatc tccttctacg acttcccggg tcggcgctgg
      241 ctggattggg ccctgatgtt gccgctggcg atgcccggct acgttctggt gttcgtgctt
      301 gtcggccagt tcggctacgc gaacccgttg cagagcagcc tgttcggcag tggcctgcag
      361 ctgcctggcc tgcgcgggcc cgccggggcg atcggcgtcc tcaccgccgt gctctatccc
      421 tacgtctatg cgctgggacg cagtgcgttc ctgggccagt cgcggcaatc tatggaggtg
      481 gcgcgcaacc tcgggcgcag ctacgggcag gcggtgcgcg acgtcgcgct gccgctggcc
      541 cggccggcgc tggccgccgg cgccgcactg gcggtcatgg aggcactcgc cgacttcggc
      601 gttgtgaacc tgctcggcta ccaggcgctg accgatgcga tttatcgcgt ctggtatggc
      661 gccttcgacc aggcggccgc gctgcagctg gccacggtgc tcgtcgcgct ggcgctgatc
      721 atggtcgcgc tggagcggct gctgcggggc cgggcgcgtt accaccagac gctggcccgc
      781 ggcgacgccg tcaccccacg tcggctgcgg ggatggcgcg ccgggctggc gacggcacta
      841 ccgtgcctgc tgctggcggt ggtattcgcc gttccggtcc tgcagctgct ggcctggtcg
      901 atcgaaacag tcttggctga ccgggtgagc gcggggttgg gccaggccgc gttcaacacc
      961 ctcctgctcg caatggctgc cgctgtcatc acggtgatca cctcgacagt cgtcgtctac
     1021 ggccagcggg tcgcaccgtc gcggatcggt gcggttgcgg cgcggctggc cgcggtcggc
     1081 tacgccgtgc cgggcacggt catcgcggta gcaatctacg tgccgctggt gtggatcgat
     1141 cgccggctga tccgcggtgt cgagggcctg ttcggcacca gcatcgggct actgttcacc
     1201 ggcaccatca tcgggctggt ctgcgcctac gtcgtgcggt tccatgccca ggcgttcttc
     1261 gccctggagt cccggatggg acggatcagc ccgaacctcg acgatgccgc acggtccctg
     1321 ggtgccggcc gggcccgggt gctcaccaag gtgcaccttc cactgctgtg gcctggagtg
     1381 ctcaccgcgg ccctgctggt gatggtggag gtcatgaagg agctcccggc caccgcgctg
     1441 ctgcgcccgc tgggtggcga cacgctggcg atcatggcct gggaagcgac caaggacgcc
     1501 cgcttcgaca ccgccgcgct gccggcgctg ctcatcgtcg cggtcggcct gctccctgtg
     1561 gtggtcctca tccgcctgtg gagccaatct ggtccgtcct cggcgactga tcccgtgaca
     1621 ccgggatagc ccggcggggc aggatcgggg cgtcccgacc tgtgcctgca accgtgttct
     1681 cctcgtggta gtcgccctca acgttgaccg accagatgtc gtggtcactg ccgcccagga
     1741 tgttttccac ggtgagcatg gcggtgagca tcgagtgatc ctggttgttg tacctgtgca
     1801 tgccgttgcg cccgacgggg tggacgttgc gggcgtggat tgccagccag ttgcgcaaca
     1861 ccgccacgtt ggtctgaaag tcatggtcat aaacgggata ggccttgggc atccgaacca
     1921 cgtagccagc ctccacctgt tcggcatcca ccagcccgag ggcggccagc tcccgggtac
     1981 ccagctcgac gagatcggcg tcgggcagct cccacagctc gtcaccggag aacacgaagt
     2041 actccaggcc aaggcaggtg cgcc
//
LOCUS       DATADN010000286         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_487694, whole genome shotgun sequence.
ACCESSION   DATADN010000286 DATADN010000000
VERSION     DATADN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487694"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VHH53_03810"
     CDS             <1..215
                     /locus_tag="VHH53_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HEX2289322.1"
                     /translation="HAELTRVFFLQLRVVGSTMGTRDELRDLLSFLDISGVRPHIGAV
                     LPISDAKQGFRTMLNGNTAGKIVFTH"
     gene            complement(242..>2050)
                     /gene="lepA"
                     /locus_tag="VHH53_03815"
     CDS             complement(242..>2050)
                     /gene="lepA"
                     /locus_tag="VHH53_03815"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX2289323.1"
                     /translation="KTFTPPELIRNFCVIAHIDHGKSTLADRMLQLTGVLDARSYRAQ
                     YLDRMDIERERGITIKAQNVRLPWRVGDTEHVLHLIDTPGHVDFTYEVSRALEACEGA
                     VLLVDAAQGIEAQTLANLYLALEKDLRIIPVLNKIDLPAAEPDRYAAELAHIIGCQPD
                     DVLRVSAKTGQGVRELLDVVVEQVPAPVGDSAAPVRAMIFDSVYDSYRGVVTYIRVMD
                     GRITPRDRIRMMSTNATHELLEVGIISPDQKPCDGLGVGEVGYLMTGVKDVRQSRVGD
                     TVTSERHGATVALPGYRTPKPMVFAGLYPMDGSDYPELRDALDKLRLNDAALSYQPET
                     SAALGFGFRCGFLGLLHLEITRDRLEREFGLDLISTAPNVVYRVVRDDGSEQTVTNPA
                     DWPDGKAAEVYEPVVSCTLIAPSEFVGTIMELCQGRRGTLLGMDYLSESRVELRYTLP
                     MAEIVFDFFDALKSRTRGYASMDYEEAGEQAADLVKVDILLQGEPVDAFSAIVHREAA
                     YSYGTSMATRLRELIPRQQFEVPIQAAIGSRIIARETIRAIRKDVLAKCYGGDITRKR
                     KLLERQKEGKKRMKTIGRVEVPQEAFVAALSTDGRK"
BASE COUNT          335 a          688 c          673 g          354 t
ORIGIN      
        1 accacgccga gttgacccgg gtgttcttcc tgcagctgcg ggtggtgggc tcgaccatgg
       61 gcacccgcga cgaactgcgc gacctactgt ccttcctgga catctccggg gtacgcccgc
      121 acatcggcgc agtactaccg atatccgacg ccaagcaggg attcaggacg atgctcaacg
      181 gcaacaccgc cggcaagatc gtcttcaccc actgaccctt ccgtcacgcg gtcgggttgg
      241 gttacttgcg gccgtcggtg gagagggcgg ctacgaaggc ttcttggggg acttcgaccc
      301 ggccgatggt cttcatgcgc ttcttgccct ccttttgccg ctccaggagt ttgcgcttgc
      361 gggtgatgtc cccgccgtag cacttggcca gcacgtcctt gcgaatagcg cggatggtct
      421 cccgggcgat gatccgggag ccgatcgcgg cctggatcgg cacctcgaac tgctggcgcg
      481 ggatgagctc acgcagcctg gtcgccatgg aggtgccgta gctgtaggcg gcctcacggt
      541 gcacgatcgc ggaaaacgcg tccaccggct caccctgtag caggatgtcc accttcacca
      601 gatcggcggc ctgctcgccg gcctcctcgt aatccatcga ggcataccca cgggtgcggg
      661 acttcagcgc gtcgaagaag tcgaacacga tctcggccat cggcagggtg tagcgcagct
      721 ccacccgcga ctccgagaga tagtccattc ccagcagggt gccgcggcgg ccctggcaga
      781 gctccatgat cgtgccgacg aactccgagg gcgcgataag ggtgcagctg accaccggct
      841 cgtacacctc ggcggccttg ccgtcgggcc agtccgcagg attggtgacg gtctgctcgc
      901 tgccatcatc acggacgacc cggtagacca cgttgggcgc ggtggagatg aggtcgagcc
      961 cgaactcgcg ctccaaccgg tcgcgggtga tctccaggtg cagcaggccg agaaacccgc
     1021 agcggaaacc gaacccgagc gcagccgacg tctccggctg gtagctcaga gcggcatcgt
     1081 tgagccgcag cttgtccagg gcatcacgca gctccgggta gtccgagccg tccatcgggt
     1141 acagcccggc gaacaccatc ggcttgggag tgcggtagcc gggcagcgcc accgtggcac
     1201 cgtggcgctc gctggtcacc gtgtccccga cccgggactg ccggacgtcc ttgaccccgg
     1261 tcatgaggta gccgacctcg ccaacgccca acccatcaca cggcttctga tcaggcgaga
     1321 tgatgccgac ctcgagcagc tcgtgggtgg cgttggtgga catcatccgg atccggtccc
     1381 gcggggtgat ccgaccgtcc atcactcgga tgtaggtgac cacgccccgg taggagtcat
     1441 acaccgagtc gaaaatcatc gccctgaccg gcgcggcgct gtcgcccacc ggagccggga
     1501 cctgctcaac caccacgtcg agcaactcac gcacaccctg cccagtcttc gcactgaccc
     1561 gcagcacgtc atcgggctgg cagccgatga tgtgcgccag ctccgcggcg tagcggtccg
     1621 gctcggctgc cgggaggtcg atcttgttga gcaccgggat gatccgcaga tccttctcca
     1681 gcgccaggta caggttcgcg agcgtctgcg cctcgatccc ctgcgcggcg tcgacgagca
     1741 ggaccgcgcc ctcgcacgcc tcgagcgcgc gggagacctc gtaggtgaag tcgacgtggc
     1801 cgggcgtgtc gatcaggtgc agcacgtgct cggtgtcccc gacccgccag ggcagccgga
     1861 cgttctgtgc cttgatggtg attccgcgtt cccgttcgat gtccatccgg tccaggtact
     1921 gcgcccggta actgcgtgcg tcgagcacgc cggtgagctg cagcatgcgg tcggccaggg
     1981 tggacttgcc gtggtcgatg tgcgcgatga cgcagaagtt gcggatcagc tcgggagggg
     2041 tgaacgtctt
//
LOCUS       DATADN010000287         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_487982, whole genome shotgun sequence.
ACCESSION   DATADN010000287 DATADN010000000
VERSION     DATADN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487982"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..728
                     /locus_tag="VHH53_03820"
     CDS             159..728
                     /locus_tag="VHH53_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX2289324.1"
                     /translation="MVPDTRPDQHVPEPRAAPDASDVAKDASGQAGLTNEQRLEAERR
                     KLGEREQQLIASFGDRDITSDRADQAQLLEGVHELQWVRDRIAEISQLLADLRQPRGQ
                     VRSGRIEVGTTVDLEYHDGSREAVWLGSPGLAHTDDAVVTPSSPLGQALLGHGVGDSV
                     DYTTPAGPQRVRVLAVRPAQLGSEAGTSR"
     gene            1132..>1781
                     /locus_tag="VHH53_03825"
     CDS             1132..>1781
                     /locus_tag="VHH53_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2289325.1"
                     /translation="MLIGDIDIHPVADGTFRASPAYFGAHARPDGHEDLFARHGMAWL
                     PIGCFLVRTGDRTILVDAGMGPEMRDDGPRRLLVGGQLLVGLRAVGVTPPDITDVVCT
                     HLHADHCGWLFDRDAAPVFTHATIWFGAHDWDHFVADPDVWMFDHIRHGFRAASQRGD
                     RLRPVNRDTTIAPGVTLTMTPGHTPGHLSVVVSSAGRRALLLGDAIVCPVQLDEPT"
BASE COUNT          302 a          635 c          561 g          283 t
ORIGIN      
        1 gctggacaga ccacctgcag cactgaccgg tgatagccgg ggctgatgct cgtgtggtga
       61 ttatttccgg cggtgatgcg gtcgttacgc ccagtgcgtg caggccaccc tatgttatgg
      121 cggttgaaac ccagtcgtta cctgttcatc attgtggcgt ggtgcccgat acccgaccgg
      181 accagcatgt acccgagccg agggcagcac ctgacgcgag cgacgtcgcc aaggatgcaa
      241 gcgggcaggc cgggctgacc aacgaacagc ggctggaggc cgagcggcgc aagctaggtg
      301 agcgagaaca gcagctcatc gccagcttcg gtgataggga cataaccagt gaccgcgcag
      361 accaagctca actgctcgaa ggcgtccatg agctgcaatg ggtccgcgac cgtatagctg
      421 agatcagcca gctgttggcc gatctccgac agccgcgggg acaggtgcgg tctggtcgga
      481 tcgaggtcgg caccacggta gacctggaat accacgacgg gagccgagag gcggtctggt
      541 tgggatcccc ggggttggcc cacaccgatg acgcggtggt gacgccaagc agcccgctgg
      601 gacaagcgct gctcgggcat ggggtgggcg atagcgtcga ctacaccacg ccggcgggtc
      661 cacagcgggt ccgtgtactg gcagtgagac ccgcgcagtt gggtagtgag gctggcactt
      721 cgcgctgagc cagggctgtt cttgagtggc catctactgc ctcaggccct tgaccgcctc
      781 cgcaatgatg atcggcgcgg ttacctactc gccaccgtta cgctgctcgc cggagccgct
      841 cggagcgagg cttgccagag tcgcgttggc cgctcgcacc gccccccgtc agcgagaagc
      901 tcgccccgac aacaaggtct actcatctcc ggcggtgcgc ccgttgtcga ggtcgccgaa
      961 agctatgggt atcgcgcaag acggtgcacg cctggctgcg ccgttaccgc tagaggcctg
     1021 tcagggttag ctgacggtcg caccgcctcc atcaccaccc gggacagctg gccgccaaga
     1081 tcgaagcacg agtgcgaccg tcgaccgacg atcgcacccc cgaggagacc aatgctgatc
     1141 ggtgacatcg acatccaccc cgtcgccgac gggaccttcc gggcgtcgcc cgcttacttc
     1201 ggcgcccacg cccgccccga cggccacgag gacctcttcg cccgccacgg aatggcctgg
     1261 ctgcccatcg gctgtttcct cgtgcgaacc ggtgaccgga cgatcctcgt cgacgccggc
     1321 atgggacccg agatgcgcga cgatggcccc cgtcggctcc tcgtcggcgg ccagctcctg
     1381 gtcgggctcc gcgccgtcgg cgtcacccca cccgacatca ccgacgtcgt ctgcacccac
     1441 ctgcacgccg accactgcgg ctggcttttc gacagggacg ccgccccggt cttcacccac
     1501 gccaccattt ggttcggcgc gcacgactgg gaccacttcg tcgcagaccc cgacgtgtgg
     1561 atgttcgacc acatccgaca cggcttccgc gccgcctccc agcgcggcga ccgcctccgg
     1621 cccgtcaacc gcgacaccac catcgcgccc ggcgtcaccc tgaccatgac acccggacac
     1681 acccccggac atctcagcgt cgtcgtctcc tccgcaggcc gccgcgcact gctgctcggt
     1741 gacgcgatcg tctgcccggt acagctcgac gaacccacct g
//
LOCUS       DATADN010000288         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1093178, whole genome shotgun sequence.
ACCESSION   DATADN010000288 DATADN010000000
VERSION     DATADN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093178"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VHH53_03830"
     CDS             complement(<1..546)
                     /locus_tag="VHH53_03830"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2289326.1"
                     /translation="MTAGRGEVDPARRGELSAAVARLRERIAAACAAASRDPAGVRLL
                     AVTKTYPAADVALLADLGVHDIAEARDQEAVPKVAELTALRSEAAVRWHMVGRLQRNK
                     ARSVARWARQVQSVDSARLVDALDRASRAAQDVGERDGALGVLVQASVDGNPTRGGCP
                     LPELEALADRVAAASGLGLEGV"
     gene            complement(554..1291)
                     /gene="pgeF"
                     /locus_tag="VHH53_03835"
     CDS             complement(554..1291)
                     /gene="pgeF"
                     /locus_tag="VHH53_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX2289327.1"
                     /translation="MNAVRIRRVVTTRHGGVSAAPYASFNLGAHVGDRSEAVAANRER
                     LAAGVGLPARRLIWMEQVHGRTVTAVDGPRVDPVPATDALVTAEPELAVAVLVADCVP
                     VLLADPVEQVVAAVHAGRVGARTGVLPATLEAMRKLGARTEQVEVLLGPSACGQCYEV
                     PAAMQADVEARLPGSACRTRRGTAGLDLRAGLWRQLTAAGVARIGIDPRCTVEDPALY
                     SYRREGVTGRLAALTWFEGCGSAQDSG"
     gene            complement(1288..>2220)
                     /locus_tag="VHH53_03840"
     CDS             complement(1288..>2220)
                     /locus_tag="VHH53_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2289328.1"
                     /translation="VASIARKLGALTTGVVTRPFTFEGKRRAGQAEDGITELRNECDT
                     LIVIPNDRLLQLGDANVSLMDAFRSADEVLLSGVQGITDLITTPGLINLDFADVKSVM
                     SGAGSALMGIGSSRGEGRSVQAAEKAINSPLLEASMEGAHGVLLSIAGGSDLGLFEIH
                     EAASLVQESAHPDANIIFGTVIDDSLGDEVRVTVIAAGFDSGGPTRKKLDPSPVTART
                     VASAQSGKVGQASRSSQPQPSMPAPAAGSAGFPPAATSQRQTTPNPTAWQPAAGPRRP
                     GGAVPSRSVPGRGVPVAGDFDDDDDVDVPPFMRS"
BASE COUNT          355 a          824 c          729 g          312 t
ORIGIN      
        1 cacaccctcc aaccccagtc cagaggcggc tgccacccgg tctgccagtg cctcgagctc
       61 gggcagcggg cagccgccac gcgtgggatt gccgtccacg ctggcctgca cgagcacgcc
      121 gagagcaccg tcgcgttccc cgacgtcctg tgcggcgcgg gaagcccggt ccagcgcatc
      181 gaccagtcgg gccgaatcca ccgactgcac ctgcctcgcc caccgggcca ccgaacgggc
      241 tttgttgcgc tgcaaccggc cgaccatgtg ccagcgcacc gcagcttccg agcgaagcgc
      301 ggtgagctcg gccaccttgg ggacagcctc ctggtcgcga gcctcggcga tgtcgtgcac
      361 tccgaggtcg gcgagtagcg ccacgtcggc ggcggggtag gtcttggtga cggcgagcag
      421 ccgcacgcca gcgggatctc ggctcgccgc ggcacacgcg gcggcgatgc gctcccgaag
      481 ccgggccacc gccgctgaca gctcaccgcg gcgggccggg tcaacctcac cacgaccggc
      541 tgtcacgacc cgatcatccc gagtcctgcg cagacccgca cccctcgaac caggtcagcg
      601 cagcaagtcg gccggtgacg ccctcgcggc gatagctgta caacgccggg tcctcgactg
      661 tgcatcgagg gtcgatgccg attcgggcca cgccggcggc ggtcagctgg cgccataacc
      721 cggcccgtag gtccaacccg gcggtccccc gccgggtccg gcaggcgctg cccggcaagc
      781 gagcctccac gtcggcctgc atcgctgccg gtacctcata gcactgcccg cacgccgacg
      841 gaccgagcaa cacttccact tgctcggtcc gcgcgccaag cttgcgcatg gcctccagcg
      901 tcgctggcag caccccggtc cgggcgccca cccgccccgc gtgcacagcg gcgacgacct
      961 gttcgaccgg gtccgcaagc agcaccggca cacagtcggc gaccagcacg gccaccgcta
     1021 gctcgggttc ggcagtgacc agcgcgtcgg tagccggtac cggatccacc cgcgggccgt
     1081 cgaccgcggt gacggttcga ccgtgcacct gctccatcca gatcaaccgg cgcgcgggca
     1141 gtcccacacc ggcggccagc cgctcccgat tcgccgctac cgcctcggac cggtcaccga
     1201 cgtgcgcacc aaggttgaag gaggcgtatg gcgcagcgga gaccccgccg tgccgggtgg
     1261 tcaccacccg gcggatgcgg acagcgttca cgacctcatg aacggcggaa cgtccacgtc
     1321 atcatcatcg tcgaagtccc cggccaccgg tacccctcgg cccggtacag aacggctcgg
     1381 caccgcgcca ccaggtctgc gcgggcctgc cgcaggctgc catgcggtgg ggttgggcgt
     1441 ggtctgacgt tgtgaagttg cggccggtgg gaacccagcg gatccagccg caggcgcggg
     1501 catcgacggc tgcggctgtg acgacctgct ggcttggccg acctttcccg attgcgcgct
     1561 ggccaccgtc cgcgcggtga ccggcgaggg gtccagcttc ttgcgcgtgg gcccaccaga
     1621 gtcgaaaccg gcggctatca ccgtgacccg cacttcatca cccaacgagt cgtcgatcac
     1681 ggtgccaaag atgatgttgg catcgggatg cgcggactcc tgcaccagcg aagcggcctc
     1741 gtggatctcg aacaggccca gatccgagcc gccggcgatg gacagcagga caccatgggc
     1801 gccctccatc gaggcttcca gcaacgggga gttgatggcc ttctcggcgg cctgcaccga
     1861 tcgcccctct cctcgggagg aaccgatgcc catcagcgcg ctacccgcac cggacatgac
     1921 acttttcacg tcggcgaagt ccaggttgat cagaccaggg gtggtgatga gatcggtgat
     1981 gccctggaca ccggacagca gcacctcgtc agccgagcgg aacgcatcca tcaggctcac
     2041 gttggcgtca ccgagctgca gcagccggtc attagggatc acgatcaggg tgtcgcactc
     2101 gttacgcagc tcggtgatgc cgtcctcggc ctgcccggcg cgccgcttgc cctcgaaagt
     2161 gaacggtcgg gtgacgacac cggtggtcag cgcacccagc ttgcgggcga tgctggccac
//
LOCUS       DATADN010000289         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1392776, whole genome shotgun sequence.
ACCESSION   DATADN010000289 DATADN010000000
VERSION     DATADN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392776"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..672
                     /locus_tag="VHH53_03845"
     CDS             331..672
                     /locus_tag="VHH53_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2289329.1"
                     /translation="MARAATTSDAFNAIAEPQRRDILALLRAGERPVTDMAQDLGMSQ
                     SRASKHLRVLREVGLVRVRVAGKQRLYGLDARGLRPVHEWVGGFERFWNESFDRLDTY
                     VQDLKQTRQEE"
     gene            676..1182
                     /locus_tag="VHH53_03850"
     CDS             676..1182
                     /locus_tag="VHH53_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2289330.1"
                     /translation="MAEARQETQAEPATADREIVISRVIDGPRELVFEAFTEVRHLSR
                     WWGPEGFTTTTRSFEFRVGGEWDFVMHGPDGTDYQEWITWTEIAPPERIALLHGESRD
                     HPNAFESVLTFAPDGAATRIVMRTVFPTKELRDQTVEKYHAIEGGEQTLSNLAAYVAE
                     IAQKKEGR"
     gene            1182..1364
                     /locus_tag="VHH53_03855"
     CDS             1182..1364
                     /locus_tag="VHH53_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289331.1"
                     /translation="MAGKVFFSVSMSLDGFSAPESMEGILATPEQREQDPRLERWMAQ
                     WMELQQWIFPLRFFRG"
     gene            1427..>1818
                     /locus_tag="VHH53_03860"
     CDS             1427..>1818
                     /locus_tag="VHH53_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX2289332.1"
                     /translation="MTRTRIHNLSISLDGFATGEGQSADAPMGHAGRRLAEWLFATRF
                     GAPILGRKGGTAGVDDAFAQQHEPGIGAEIMGAGKFGPPDWQDDADWRGWWGPNPPFH
                     TPTFVLTHRPGPPIEMEGGTTFHFLDASP"
BASE COUNT          320 a          587 c          638 g          273 t
ORIGIN      
        1 cccacggtcc atacttgcag aagcctccga gcgcccttat tcacccgagt gcgtggaagg
       61 cttgttctcc gaacttcggg tgtaggaggt tctgagaagt tcgccggcgc gtccccgtcc
      121 gtactacgat ccctgcgttg cgaaaaacct tgcgggcccg ccctgccgcg ggccagcttt
      181 gcccgagggg agcatcaggc atgaggcgca cacgcttgaa tcagcaggcc accgcggcca
      241 actgcgaata gcctgcaatc gtcatccgac aagactcgcc cgattcccaa ccggcttgac
      301 attttcccat ataggaatat gatggatgcc atggcacgag cagcgacgac gtcggacgcg
      361 ttcaacgcga tcgccgagcc gcagcgccgg gacatcctgg cgctgctgcg ggcgggtgag
      421 cggccggtga ccgacatggc ccaggacctg gggatgagcc agtcgcgggc gtccaagcac
      481 ctgcgggtgc tccgggaggt ggggctggtg cgggtccgcg tggcgggcaa gcagcgcctc
      541 tacggcctgg acgcccgcgg gctgcggccg gtccacgagt gggtcggcgg gttcgagcgg
      601 ttctggaacg agagtttcga ccggctggac acctacgtgc aggacctcaa gcagacacgg
      661 caggaggaat gaccgatggc agaggcaagg caagaaacgc aggcggagcc ggcgacggcc
      721 gaccgggaga tcgtgatctc ccgggtcatc gacggcccgc gggagctggt gttcgaggcg
      781 ttcaccgagg tccggcacct gtcgcggtgg tggggtccgg aggggttcac caccaccacg
      841 cggtccttcg agttccgcgt cggcggggag tgggacttcg tgatgcacgg accggacggg
      901 acggactacc aggagtggat cacctggacc gagatcgccc cgccggagcg gatcgcgctg
      961 ctgcacggtg agtcccgcga ccacccgaac gccttcgagt cggtcctgac cttcgcgccg
     1021 gacggtgcgg cgacccggat cgtgatgcgc acggtgttcc ccaccaagga actgcgcgac
     1081 cagacggtcg agaagtacca cgcgatcgag ggcggcgagc agacgctgag caacctggcg
     1141 gcctacgtcg ccgagatcgc ccagaagaag gagggccgct gatggccggg aaggtgttct
     1201 tcagcgtgtc gatgtcgctg gacggcttca gcgcgcccga gtcaatggaa ggcattctcg
     1261 ccacgcccga gcagcgggag caggacccga ggctggagcg ctggatggcg cagtggatgg
     1321 aactgcagca gtggatcttc ccgctgcggt tcttccgggg ttaggcgatg aatccctgct
     1381 gtagccgcag tcaacacgat cagacatccg acccggaggc aggcatatga cacgcacacg
     1441 gatccacaac ctctccatct cgctggacgg cttcgccacc ggtgagggcc agagcgcgga
     1501 cgcgcccatg ggtcacgccg gcagacggct ggccgagtgg ctgttcgcca cccgcttcgg
     1561 tgccccgatc ctgggacgca agggcggcac cgccggcgtc gatgacgcgt tcgcccagca
     1621 gcacgagccg ggtatcggcg ccgagatcat gggtgcgggg aagttcggtc cgccggactg
     1681 gcaggacgac gccgactgga gagggtggtg gggccccaac ccgcccttcc acacgccgac
     1741 gttcgtgctc acccatcggc cgggtccccc gatcgagatg gagggcggca ccaccttcca
     1801 cttcctcgac gcctcacc
//
LOCUS       DATADN010000290         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_488474, whole genome shotgun sequence.
ACCESSION   DATADN010000290 DATADN010000000
VERSION     DATADN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488474"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1679
                     /gene="guaA"
                     /locus_tag="VHH53_03865"
     CDS             3..1679
                     /gene="guaA"
                     /locus_tag="VHH53_03865"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX2289333.1"
                     /translation="MGGTAGGTRGELFGVPMTAHPPRSGGLVNGIECYNTVSHSRVVL
                     VVDFGAQYAQLIARRVREAQVYSEVVPHTTPVAEILGRKPAAIVLSGGPASVYAEGAP
                     RVDPALFEARVPVLGICYGFQAMADALGGVVAQTGTREYGRTDLQVLDGGGLLHRELP
                     ARGPVWMSHGDAVTAAPDGFAVTARSAGAPVAAFEDTGRRLGGVQYHPEVVHSPHGQE
                     VLRRFLHELAGIRPNWTTANVIDETVAAVRAQTQGAKLICGLSGGVDSAVAAALVHKA
                     VGDDLTCVFVDHGLLRAGERAQVERDYVAATGVRLVTVDARDRFLRTLARVIDPEQKR
                     KIIGREFIRVFEGAARDLKAEAGRHGEEIRFLVQGTLYPDVVESGGGSGTANIKSHHN
                     VGGLPEDLKFELIEPLRALFKDEVRRVGLELGLPETIVHRQPFPGPGLAIRIIGEVTA
                     AHLETLRAADAIAREELTAAGMDRDIWQCPVVLLAQVRSVGVQGDGRTYGHPVVLRPV
                     SSEDAMTADWSRVPYDVLARISGRITNEVPEINRVVLDVTSKPPGTIEWE"
     gene            complement(1663..1992)
                     /locus_tag="VHH53_03870"
     CDS             complement(1663..1992)
                     /locus_tag="VHH53_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289334.1"
                     /translation="MLVIPGALAVIYGDDVSRALATIDTVSRAMGVALLSGGITTITG
                     IARGRSLVEVIGLSLLAVGCGIYGLGVFLGLGSLERWRVAGFLQALATVRRVVTLAAV
                     APIIPIR"
     gene            2439..3332
                     /locus_tag="VHH53_03875"
     CDS             2439..3332
                     /locus_tag="VHH53_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycobacterial-type methylenetetrahydrofolate
                     reductase"
                     /protein_id="HEX2289335.1"
                     /translation="MTLNTIALELVPPNVEHGTQRAVDEAHKVLEHSKTFGIQGRIKH
                     VMIPGLIEEEDDRPVEMKPKLDVLDFWSAIQPELPGVSGLCTQVTAFMDEPALGERLA
                     TLRGAGIDGVIFVGVPRTMKDGEGSGVAPTDALSLYDQLVRNRGVILIPTRDSEVGRF
                     NFKCNQGATFAMTQLLYSDAIVSFLTDFARQTEHRPEILLSFGFVPKFENRIRLINWL
                     IQDPGNDAVAQEQALVAKLADSEPDAKRKIMLDLYKRVIDGVAELGYPLSIHLEAPYG
                     VSAPAFETFAEMLAYWAPDKN"
BASE COUNT          610 a         1064 c         1159 g          550 t
ORIGIN      
        1 acatcggggg tactgcgggc ggcacccgcg gggagctgtt tggggtgccg atgaccgcgc
       61 atcccccgcg cagtgggggc ctcgtgaatg gcattgagtg ctataacacc gtttcccact
      121 cacgagtagt tctggtggtg gacttcgggg cgcagtatgc gcagctgatc gctcgtcggg
      181 tccgcgaggc tcaggtgtat tccgaggtgg tgccgcacac cacaccggtc gcggagatcc
      241 tcggacgcaa gccggcggcg atcgtgctct cgggtggtcc ggccagcgtg tacgccgagg
      301 gtgccccccg cgtcgacccg gcgctgttcg aggcccgtgt accggtgctg ggtatctgct
      361 acggcttcca ggcaatggcc gacgcgctgg gcggcgtggt ggcgcagacc ggcacccggg
      421 agtacggacg caccgatctg caggtgctcg acggaggcgg cctgctgcac cgcgagctgc
      481 ctgcgcgtgg tccggtgtgg atgagccacg gtgacgctgt caccgccgcg ccagacgggt
      541 tcgccgtgac cgcacgatcg gcgggtgcac cggtagcggc gttcgaggac actggtcggc
      601 ggctgggcgg ggtgcaatat cacccggagg tggtgcactc cccgcatggc caggaggtgc
      661 tgcgccggtt cctgcacgaa ctcgccggta tccggccgaa ctggaccacg gccaatgtca
      721 tcgacgagac cgtggctgcg gtccgggccc agacccaggg cgccaagctg atctgcggcc
      781 tttccggtgg ggtggattcc gcggtcgccg ctgcgctggt gcacaaagca gtcggggatg
      841 atctgacttg cgtgttcgtc gaccacggct tactgcgggc cggcgagcgc gctcaggtgg
      901 agcgggatta cgtggccgcg acgggagtcc ggttggtcac cgtcgacgcc cgggaccggt
      961 tcctgcggac gctggccagg gtgatcgatc cggagcagaa acgcaagatc atcgggcggg
     1021 agttcatccg ggtcttcgaa ggtgcggctc gggatctgaa ggccgaagcg ggccggcacg
     1081 gcgaggagat ccgcttcctc gtgcagggca ccctgtatcc ggacgtggtg gagtccggcg
     1141 gtggatcggg caccgccaac atcaagagcc atcataacgt cggcggcctg cccgaggacc
     1201 tgaagttcga gctgattgag ccgctgcgag cgctgttcaa ggacgaagtg cgtcgggtag
     1261 ggctggaact ggggctcccg gaaactatcg tgcaccgcca gccgttcccg ggaccgggcc
     1321 tggcgatccg gatcatcggc gaggtcactg ccgcgcacct ggagacgctg cgtgccgccg
     1381 atgcgatcgc ccgggaggag ctcaccgcgg ccgggatgga ccgcgacatc tggcagtgcc
     1441 cggtggtgct gctggcgcag gtgcgcagcg ttggcgtaca gggcgacggg cggacctacg
     1501 ggcatccggt ggtgctgcgc ccggtgtcca gcgaggatgc gatgaccgca gactggtccc
     1561 gggtgcccta tgacgttctg gcccgcattt ccggccggat caccaacgag gtacccgaga
     1621 tcaaccgggt ggtgctggac gtgacgagca aaccgcccgg cactatcgaa tgggaataat
     1681 cggagcgaca gcggccaggg tcaccacacg ccgaacggtg gccagggctt gcaagaaacc
     1741 cgctacccgc caccgctcca gcgaaccgag gccgaggaat actcccaggc cgtagatgcc
     1801 gcaaccgacg gcgagcaacg agagaccaat gacctccacc agggagcgac cgcgggcaat
     1861 acccgtgatg gtggtgatgc caccggagag caacgcgacg cccatggcac gactgacggt
     1921 gtcaatggtc gccagcgccc gggatacatc atcgccgtag ataacagcga gcgcgccagg
     1981 gatcaccagc acggccgcga gtacaacggt gaacggacga tctacccacc aatcgaagcc
     2041 gctggactac tccaatcctc tgagtgctgc tccacgagga ggggcctcgc ggcttgcggt
     2101 ggccagcgcc tgtggcgtgt gctcccactc gctacgggta gacggcttgc cgtcgggggt
     2161 aggcgggtcg accggatcgg aagccgatca tcggcggcca tgacgctcca ttcgtcgaga
     2221 gttgtagtca taactctatg acacggtccg tgacagcaag tcaccgatac gaactgccca
     2281 gtatgtcccg cgcttcacgt tggacgaccg cggactggtc ctggattccc tatgacatgc
     2341 tgccccgcct ctccgaccat cgaatgggag tagtcaacta gggacacggg gaagaccgat
     2401 ccgtatcatc aagatccgga gcgggaggag ccaacgtcgt gaccctcaat accatcgccc
     2461 ttgagctcgt tccgcctaac gtcgagcacg gaacgcagcg ggccgtcgac gaggcgcaca
     2521 aggtactgga gcactccaag accttcggca tccaaggccg gatcaaacac gtgatgatcc
     2581 cgggactgat cgaggaggag gacgaccggc cggtcgagat gaagccgaag ctcgatgtgc
     2641 tcgacttctg gtcggccatc cagccggagc tgcccggggt aagcggacta tgcacgcagg
     2701 tcaccgcatt catggatgag ccggcgctgg gtgagcggct ggcaaccctg cgaggtgccg
     2761 gaattgacgg tgtcattttc gtcggcgtgc cccgcacgat gaaagacggt gagggatcgg
     2821 gtgtcgcacc gactgacgcg ttgtcgctct acgaccagct ggtacgcaac cgcggtgtga
     2881 tcctgatccc gacccgggac agcgaggtgg gccggttcaa cttcaagtgc aatcagggcg
     2941 ccaccttcgc tatgacgcag ctgctgtatt ccgacgcgat cgtaagcttc ctgaccgact
     3001 tcgcccgaca gaccgagcac cggccggaga tcctgctctc attcgggttc gtcccgaaat
     3061 tcgagaatcg gattcgcttg ataaactggc tgatccagga cccgggcaac gacgccgtcg
     3121 cccaggagca ggccctggtc gcaaagctgg ctgacagtga gcccgacgcg aagcgcaaga
     3181 tcatgctcga cctgtacaag cgcgtgatcg acggtgtcgc agaactcgga tacccgctga
     3241 gcatccacct cgaggccccc tacggcgtct cggcgcctgc gttcgagacc ttcgccgaga
     3301 tgctcgccta ctgggccccc gacaagaact gaacccggtg ttgccccacc tggactggtc
     3361 cgcgctgctt cacgaggtca ccg
//
LOCUS       DATADN010000291         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_634250, whole genome shotgun sequence.
ACCESSION   DATADN010000291 DATADN010000000
VERSION     DATADN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634250"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VHH53_03880"
     CDS             <1..570
                     /locus_tag="VHH53_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289336.1"
                     /translation="LLIGGPLVDRVDRLTNSATRTMDAATAAADAAADALDGFDASLQ
                     EARDSATEAAVLSRETAGTVDALAAAMSLSVLGSQPLLPLADEFNATASRMRELGDNL
                     EEVGSALATNRDDLAAVSGEMRELAGSMEELGGRIGEERTSGDIPLTWLYYGFLVWQV
                     LPIMAAAIGSAWLFRHMGVTSPSAGSGAA"
     gene            complement(536..>1670)
                     /locus_tag="VHH53_03885"
     CDS             complement(536..>1670)
                     /locus_tag="VHH53_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289337.1"
                     /translation="VVRRPGVLASLVLGPFLIMLLFGIGYTGSRSPFRTEIVVPPDSP
                     LPRDPEFYAELAQGRIEVVGVNEEQESSERRLRNRQIDLMVVAPADAVGQLRSGNQTE
                     ILVAWNQVDPVYDGLAQLAVSTMVSELNAEIIRQAAAEGIELTEGELGPRVTDITPEV
                     IAKPTTAVTENLAPTDPTVLNFFAPAVLALILQHLAVTLTALSMVRERLSGQMDLFRV
                     APVNSMEVLVGKYIAYAVLTLLVGGVVGALMVFVLGVPVLAGYLVAIGIVLLLAFASL
                     GVGLLISLVADSERQAVQLSMLVLLASVFFSGFVLPVQDFIGAVQYFSYALPVTHGIA
                     TLQEAMLRGEVTTMWMIAALAGIGVALYLLSLFRLRQILRSAR"
BASE COUNT          294 a          572 c          576 g          228 t
ORIGIN      
        1 ctcctgatcg gcggtccgct cgtcgatcgg gtcgaccggc tcacgaactc ggccacgcga
       61 accatggacg cggccacggc ggccgccgat gctgcggccg acgccctcga cggattcgat
      121 gcgagcctgc aggaggcccg cgactcggcg accgaggcag ccgttctgag ccgtgagacg
      181 gcgggcacgg ttgacgccct tgcggccgcg atgtcgctct ccgtgctcgg ctcgcagccg
      241 ctgctgccgc tggcagacga gttcaacgcc acggccagcc ggatgcgtga actgggcgac
      301 aacctggagg aggtgggttc ggcgctggcc acgaaccgcg acgatctcgc cgccgtcagc
      361 ggggagatgc gcgagctggc cggcagcatg gaggagctgg gcggacgcat cggcgaggag
      421 cggacgtcag gtgacatccc gctcacctgg ctgtactacg gcttcctggt ctggcaggtg
      481 cttccgatca tggccgcggc catcggttcg gcctggctct tccgccacat gggggttacc
      541 tcgccgagcg caggatctgg cgcagcctga acagggacag caggtagagg gcaacgccaa
      601 tcccagccag ggcggcgatc atccacatcg tcgtgacctc gccgcgcagc atggcctcct
      661 gcagcgtcgc gatgccatgc gtcacgggca gcgcgtacga gaagtactgc accgccccga
      721 tgaagtcctg gacaggcagc acgaacccgc tgaagaagac cgatgcgagc agcaccagca
      781 tggacagctg cactgcctgg cgctccgagt cggccaccag cgagatcagc aggcccacgc
      841 ccagcgaagc aaacgccagc aggagcacga tgccgatcgc aaccaggtag cccgccagca
      901 ccggcacgcc gagcacgaag accatcagtg cgccgaccac gccaccgacc aggagcgtca
      961 gcaccgcgta ggcaatgtac ttgccgacca gcacctccat ggaattcacc ggcgccacgc
     1021 ggaacaggtc catctggccg ctgaggcgct cacggaccat cgacagcgcg gtcagcgtca
     1081 ccgccaggtg ctggaggatg agcgccagca ccgccggggc gaagaagttg aggacggtcg
     1141 gatcggtggg cgccaggttc tcggtgaccg ccgtggtggg cttggcgatg acctcgggcg
     1201 tgatgtcggt gacccgcggt cccagctcgc cctcggtcag ctcgatgccc tcggccgcgg
     1261 cctgccggat gatctcggcg ttcaactcgc tcaccatggt cgaaaccgcc agctgggcca
     1321 gcccgtcgta gaccgggtcg acctggttcc aggccaccag gatctcggtc tggttgccgg
     1381 accggagctg gcccacggca tcggccgggg caacgaccat gaggtcgatc tggcggttgc
     1441 gcagccggcg ctcgctggac tcctgctcct cgttgacgcc caccacctcg atgcgtccct
     1501 gggccagctc ggcgtagaac tcagggtcgc gggggagcgg cgagtcgggc gggacgacga
     1561 tctcggtacg gaatggcgaa cgcgatcccg tgtagccgat gccgaacagg agcatgatca
     1621 ggaacggccc caagaccagg ctggccagca cgccgggccg gcgaacgacc
//
LOCUS       DATADN010000292         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_634311, whole genome shotgun sequence.
ACCESSION   DATADN010000292 DATADN010000000
VERSION     DATADN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634311"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1056)
                     /locus_tag="VHH53_03890"
     CDS             complement(163..1056)
                     /locus_tag="VHH53_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2289338.1"
                     /translation="MHDSAPHAPIVVGVDGSEVSLEAVEWAAAEAAARHRRLRVVHAF
                     IWPLLRVPMGPSTYGTPASGLRADAQRILDQAVQRARQAAPGTDVTGVMPVGAPVAVL
                     LSEIEHAPLVVLGSRGLGGLSGVLVGSTGVQVVAHAPCPVIVVRPVSVATPNSLTANQ
                     LVVGVDGSSLSVAALEFGFREAVRLGIGVVAVHVTAPHVPGDGSVELLTEKNAPKQAR
                     LLAESLAGWQEKYPDVPIRRVLLRGHAGRALTKISKGVRLLVVGSRGLGGFRALLLGS
                     VSHAVLHHAHCPVAVVRTKDR"
     gene            complement(1162..1422)
                     /locus_tag="VHH53_03895"
     CDS             complement(1162..1422)
                     /locus_tag="VHH53_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEX2289339.1"
                     /translation="MKMLYRPLGLLVSVLGGLLAGAVFKTVWATASGEKEAPQATSPE
                     YSTKEVLVAAVVQGAIFAGVKAAVDRAGAKGFKKITGVELGT"
     gene            complement(1419..>1759)
                     /locus_tag="VHH53_03900"
     CDS             complement(1419..>1759)
                     /locus_tag="VHH53_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289340.1"
                     /translation="RVQELKAQAAHKAQELCAQALAKAPQLTETAQQKAQLAQRVVQD
                     KSGAAAGAVLAALVLLTLRRRSRRRANDHKAQQARGGSRTTRSRRGMRVLAVLGSLAR
                     RHRRVREENS"
BASE COUNT          313 a          642 c          530 g          274 t
ORIGIN      
        1 gtcgtagagc accggcccca atagatcgcc tagggcatgc acgagcgcat cgtgctgact
       61 ggaatgactg gacggcgggc cagccgagca gccgacgagc acggcgttgg cgtggtcaaa
      121 ccagctcttg ccagcacgtg cgctcggcaa ctgtgtcgat cgtcagcggt ccttcgtacg
      181 cacgaccgcg accgggcaat gcgcgtggtg gagcacggcg tggcttaccg aacccagcag
      241 tagcgcacga aaaccaccca gaccgcggga acccacgacg agcagacgaa cccccttgga
      301 gattttggtc aatgctcgac cggcatgccc gcgcagcagg acccgccgga tgggcacgtc
      361 cgggtacttc tcctgccaac cggcaaggga ctccgctagc aatcgggcct gcttgggcgc
      421 gtttttctcg gtgagcaact caacgctgcc atcaccgggt acgtgggggg cggtcacgtg
      481 cacagccacc acgccgatgc ccagccggac cgcttccctg aagccgaact caagcgccgc
      541 aacggagagc gaagagccat ccacccccac aaccaactga ttagcggtca ggctgttcgg
      601 agtcgcgacc gaaaccgggc gcacgacgat caccggacac ggcgcatggg ccaccacttg
      661 cactccggtc gacccgacaa gaactccgga taaaccgccc agcccacgtg agccgagaac
      721 caccagcggc gcatgctcga tctcagacaa cagaaccgcc accggcgcac caaccggcat
      781 caccccagtg acgtcggtcc ctggggctgc ttgcctggcc cgttgtacag cttggtccag
      841 aatgcgctgt gcatcggccc gcagcccact agccggcgtg ccgtaggtgg acgggcccat
      901 tggcacgcgc agcagcggcc agatgaacgc gtgcaccacg cgcagtcggc ggtgtcgtgc
      961 agctgcttcg gctgcggccc actcgaccgc ttccaggctc acttcagatc cgtcaacgcc
     1021 caccacgatc ggtgcgtgcg gtgcgctgtc gtgcatcgct tctcctcaca gctgaccgta
     1081 cggcatgaga cgagtcacat cgagccgcca cgcgcatgtt gggagcgccc tggcagtaat
     1141 actttcggcc ccagatggcc cctacgtccc aagctcgaca ccagtgattt tcttgaaacc
     1201 cttggcgcca gcccggtcca ccgcggcctt taccccggca aaaatcgcgc cctgaaccac
     1261 ggcggcgacc agcacctcct tggtgctgta ctccggcgaa gtggcctgcg gggcctcctt
     1321 ctccccggac gccgtcgccc acaccgtctt gaacaccgcg ccggcgagga gcccgccgag
     1381 cacgctgaca agcaggccca gcggcctgta gagcatcttc acgagttctc ctcccgaact
     1441 cgacggtggc ggcgcgccag cgaccccagt acggccagca cccgcatccc ccgtcgggac
     1501 cgggttgttc tggacccccc gcgggcttgc tgggctttgt gatcattcgc ccgtcggcgg
     1561 ctgcgtcggc gcagggtcag caacaccagc gcggccagca ccgcacccgc tgccgcgcct
     1621 gacttgtcct gaaccacccg ctgggccagc tgagccttct gttgcgcggt ttcggtcagc
     1681 tgcggcgcct ttgccagcgc ctgggcacac aattcctggg ccttatgggc ggcctgcgcc
     1741 ttgagctcct gtacccggc
//
LOCUS       DATADN010000293         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1393090, whole genome shotgun sequence.
ACCESSION   DATADN010000293 DATADN010000000
VERSION     DATADN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393090"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1005
                     /locus_tag="VHH53_03905"
     CDS             136..1005
                     /locus_tag="VHH53_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2289341.1"
                     /translation="MPTTEVVGVSDPVSYTAVLPVAEETVQLVARLAAKRRRCTRSRR
                     RALSCYRQAVLLLRWFLDGTRLAQLAADNAIGRSTVYRYLHEGIDALAAAAPGLRGAL
                     LAARAAGHSHVTVDGTLIRTDRCSVAGPTARADRPDRRVDLWWSGKHAAHGGNVQIIA
                     APDGWPLWTSDVRPGREHDTTALRTHAEALPLLAEWTDPAHAVLADLGYEGERAALTT
                     PIKTPAGRRLTGDERCVNLLHAATRAPAERGNSLLKTTFKALRRVSLCPWRIGAITAA
                     ALVLLHHMHGRTT"
     gene            complement(1181..1732)
                     /locus_tag="VHH53_03910"
     CDS             complement(1181..1732)
                     /locus_tag="VHH53_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289342.1"
                     /translation="MAASDENLDVDEFGLECYESSALAGLSDVEIVISGFAELMALSD
                     ADLIAFAQDDGVGVLSLESFLAPGGGWGGSEVPARRAIPIATSNGLTVTSLKRSSGSS
                     GSDHHVSQQSAFAVDVSNGTSPTPGMLRTARQIAAAMGYSWPPNGYIQTPRTGLGYRA
                     QLIYNSSVVPNHHNHVHFGVKKI"
     gene            complement(1929..2180)
                     /locus_tag="VHH53_03915"
     CDS             complement(1929..2180)
                     /locus_tag="VHH53_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289343.1"
                     /translation="MSHNRSGWTVGADRIAPRDWSGEVVQIPSGRLHAVSGPGLIAGR
                     ALCLAPVTLLDPRDWRWPDDGDEEWPLCWTCLTLTHVQS"
     gene            complement(2191..2514)
                     /locus_tag="VHH53_03920"
     CDS             complement(2191..2514)
                     /locus_tag="VHH53_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289344.1"
                     /translation="MRDPAAPLVVEAELSGAPPGRTVRLILDLGSATAEAAAVTRDLA
                     QGVLRHIKAMLTDPATALPAEARAALTALADALAEQISGGATVTELDTPLARAWRAHP
                     AGRGR"
BASE COUNT          418 a          893 c          850 g          372 t
ORIGIN      
        1 cgccgtagca gacacctaaa gatcattgag ccattcccag tagcggacgg ttggctggtc
       61 agaggggaca cgccgacggt aaggagatga ccgggacaag cgcaggacct cggtaggagg
      121 gacgtcttct cacggatgcc caccaccgag gtcgtgggcg tgtccgatcc tgtcagctac
      181 accgccgtgc tccccgtcgc cgaggagacc gtgcagttgg tggcgcggct ggccgccaaa
      241 cgccgccgct gcacccgatc ccgtcgacgg gcgctgagct gctaccggca ggcggtgctg
      301 ctgctgcgct ggttcctcga cgggacccgg ctggcccagt tggccgccga caacgccatc
      361 ggccgctcga ccgtctaccg gtatctgcac gagggcatcg acgccctcgc cgcggccgcg
      421 ccggggctgc ggggtgcgct gctggccgcc cgcgccgccg ggcattcgca tgtgacggtg
      481 gacggcacgc tgatccgcac cgaccgctgc tcggtcgccg ggccgaccgc ccgagccgac
      541 cgccccgacc ggcgggtgga cctgtggtgg tcgggcaagc acgccgccca cggcggcaac
      601 gtgcagatca tcgccgcccc ggacggctgg ccgctgtgga cctcggacgt gcgcccgggc
      661 cgcgagcacg acaccaccgc actgcgcacc cacgccgagg cgctgccgct gctggccgag
      721 tggaccgatc cggcccacgc tgtgctggcc gacctgggct acgagggtga acgggcggcg
      781 ctgaccacac cgatcaagac tcccgccggt cgccggctga ccggcgacga gcgctgcgtc
      841 aacctgctgc acgcggccac ccgggctccg gccgaacgcg gcaactccct gctcaagacc
      901 accttcaagg cgctgcgccg ggtcagcctc tgcccttggc gcatcggcgc gatcaccgcc
      961 gccgccctcg tgctgctcca ccacatgcac ggccgcacga catgatcaac tagcgagcgg
     1021 agccactact gggaatggct cagtgccgcg tccggcaacg ttcgccctta ggacgcctga
     1081 aggacagagg gcgtcccgga cgttcgatca acggtgattg gggacgaagc ctgttggccc
     1141 aaggaccgac cgcctcgtcc ggtgaccctg cacgaggtcg tcaaattttt ttgactccga
     1201 aatgtacatg gttgtgatga ttcggcacca cacttgagtt gtagatgagt tgcgcgcggt
     1261 agccgaggcc ggttcgaggc gtctggatat acccgttagg tggccagctg tagcccatgg
     1321 ctgcagcgat ctgcctcgca gtcctgagca tgccaggagt aggggatgtt ccgtttgaga
     1381 catccactgc gaatgcagac tgctgcgaga cgtgatgatc cgatccggaa ctgccactgg
     1441 accgcttgag cgacgtgacc gtcaatccgt tactcgtcgc gatgggaatc gcgcgccgag
     1501 caggcacttc agacccgccc cagcctccac ctggcgcgag gaacgactcg agactgagca
     1561 cacccacccc atcatcctgg gcgaaagcga tgaggtcggc atcggaaaga gccatgagct
     1621 ccgcgaagcc gctgatcacg atctcgacat ccgacagccc tgccaaagcc gaggactcat
     1681 aacactccag tccgaactca tcaacatcga ggttttcgtc cgaggcggcc acctggatct
     1741 cccgtctctc actattggtg cagactaggg ccgagatggg gtcccggaga acgatgcgcg
     1801 tcccgagctc caacgctccg gagtcgcctg ggcctccctg tcgccggtcc tagagcacgc
     1861 cacacggcgg taacccacag tgggccgcta agggtgaacg cgccggccgc ggtactatcc
     1921 gactgctgtc aggactggac gtgggtcagc gtgaggcagg tccagcacag cggccattcc
     1981 tcatccccgt catcgggcca gcgccagtcg cgcgggtcga gcagggtcac cggggccagg
     2041 cacagggccc ggccagcgat caggccgggg ccgctgaccg catgcagccg accggacggg
     2101 atctggacga cctcgccgga ccagtcccgc ggcgcgatgc ggtcggcgcc gacggtccat
     2161 ccactccggt tgtgactcat gcgcacttcc tcaccggccc cgcccggccg gatgggctcg
     2221 ccacgcccgc gccagcggcg tgtccaactc ggtgacggtg gccccaccgc tgatctgctc
     2281 ggccagcgcg tcggcgagtg cggtcagcgc agcgcgcgcc tcggcgggca gcgcggtggc
     2341 cggatcggtc agcatcgcct tgatgtggcg gagcacgcct tgggccaggt cgcgggtgac
     2401 cgcagcggcc tcggcggtgg cggagcccag gtcgaggatg aggcgaaccg tgcgccccgg
     2461 cggcgcgccg gacagctccg cctcgacgac gaggggggcg gccggatcac gcacgacaag
     2521 ctcctcagct cac
//
LOCUS       DATADN010000294         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_180739, whole genome shotgun sequence.
ACCESSION   DATADN010000294 DATADN010000000
VERSION     DATADN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180739"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1175
                     /locus_tag="VHH53_03925"
     CDS             279..1175
                     /locus_tag="VHH53_03925"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEX2289345.1"
                     /translation="MTGEDGLVEEVWRQQSIWSQTANRLKASIGRARSAALALTVGGA
                     VLATLATRLSAAQVTASSVLAITAAVAVGLVPVLRPWCTGRVLRDWTRARSVSEALKS
                     EIYLYLARAGQYRGEDRDRQLREATNQAQEQVTDLLRYSIDLQPVQRALPEVDDYASY
                     LRVRVDGQIDGYYRSRAQHLQKRLRQFRRVELALAVAGVVLGALAASLTQWGLSAWIA
                     VVTTISAAVAAHVGATRYEYQLIEYLRTADELSRLRREAAATTSRADLDQLVVRCERI
                     ISIQNEGWMAKLSSPSEQEPQT"
     gene            complement(1488..>1674)
                     /locus_tag="VHH53_03930"
     CDS             complement(1488..>1674)
                     /locus_tag="VHH53_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289346.1"
                     /translation="LRLSTTVAWRGLALQHRQPALQNRGIRRSGEIPADLACRADRST
                     FVVLLRGRAGRAGVCTR"
BASE COUNT          290 a          498 c          580 g          306 t
ORIGIN      
        1 catggccttg ccggcataac cccgtggcct gacgacctga tcagcggatt ttgttaccac
       61 acggcgtccc accggatgaa ttcagcatta ccaaagaagt cgtccatttg aatgaactgg
      121 aatgatgtat gacgacttct agcttctgcg gctatggacg tcaagcgccg gtgtgcgtcc
      181 gtccttgcgt ccgtgtagac gctttcggcc gagggctact cggtgatggg ttgtctgcgc
      241 ccgcgtaacc cgtgtggcgg ttgtggcagg ctgatcacgt gacaggtgag gacgggctgg
      301 tcgaggaggt gtggaggcag cagtcgatct ggtctcagac cgccaaccgg ttgaaggcct
      361 ccatcggtcg cgcccggtct gccgcgctgg cgctgaccgt cggaggcgcg gtgttggcca
      421 cactggcgac acggctgtcg gccgcgcagg tgacggcgag ttcggtgttg gccataacgg
      481 ctgcggtggc ggtggggctg gtgccggtgc tgcggccatg gtgcaccggt cgggtgcttc
      541 gggattggac tcgggcgcgg tcagtgtccg aagccctcaa aagcgagatc tacctgtatc
      601 tggcgcgcgc tggacaatac cgaggggaag accgcgaccg ccagctgcgg gaggccacca
      661 atcaggccca ggaacaggtt acggatctgc tgcggtacag cattgacctc caacccgtcc
      721 agcgagcgct gcccgaggtc gacgactatg cgtcgtatct gcgggtgcga gtcgatggcc
      781 aaatcgacgg ttattaccgg tccagggccc aacacctgca aaagcgccta cgtcagtttc
      841 gtcgcgtgga gctggcattg gccgtggccg gggtagtgct cggtgcgctt gctgccagcc
      901 tgacccagtg gggcctgagc gcctggatcg cggtggtgac cacgatcagt gccgcagtcg
      961 cagcacacgt cggggcgacc cgctatgagt atcaactcat cgagtacctg cgcaccgccg
     1021 atgagctcag tcgactgcgg cgtgaggccg cagcgaccac atcgcgagct gacctggacc
     1081 aactggtggt gcgctgcgag cgaatcatct ccatccaaaa cgagggctgg atggccaagc
     1141 tgtcgtcccc atcggagcag gagccacaaa cctagaaccc tgctaggcga gcctcacttg
     1201 gaagatcggg cagcgatcaa gatgctgacc tgcgctcatg caacctgcgg atactcggcg
     1261 gcaggtgaac tgagtgaccg ctgttgaccc cttgcgactt tgagaacggg cacgtggggg
     1321 gcacgcgctc ggtgagcacg caggtctctg cgtcctgcgt ggaagggcgt ggcggagatc
     1381 ctgcgattag aagtggaccg cgcgggattc cgggcagccc tcggcggaat cggcatatgc
     1441 gtcatctggc tctgctgctg ggatgcccgt ggtaggcctc ggggaggtca gcgggtgcac
     1501 acccctgctc gcccggccct gccgcgcagc agcaccacga aggttgagcg gtcggcgcgg
     1561 caggccagat ccgccggtat ttcaccgctt cggcgaatgc ctctgttttg tagcgccggc
     1621 tgacggtgct gaagcgccaa cccgcgccag gcgacggtcg tcgagaggcg aagg
//
LOCUS       DATADN010000295         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1393630, whole genome shotgun sequence.
ACCESSION   DATADN010000295 DATADN010000000
VERSION     DATADN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393630"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /gene="mraY"
                     /locus_tag="VHH53_03935"
     CDS             <1..578
                     /gene="mraY"
                     /locus_tag="VHH53_03935"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX2289347.1"
                     /translation="FSYLLVSAWSNAVNLTDGLDGLAAGSAAMVLVTYVIVSFWQFTQ
                     DCTVQAVPGCYTVRDPLDVAVVAAAAVGGCIGFLWWNAPPAKIFMGDTGSLAIGGLLA
                     GLSVVTRTELLLVVIGGLFVVEAMSVVIQVVVFRTTHRRPFRMAPFHHHFELAGWAET
                     TVIIRFWLLAAICCALGAGVFYSEWLNAAGG"
     gene            582..1988
                     /gene="murD"
                     /locus_tag="VHH53_03940"
     CDS             582..1988
                     /gene="murD"
                     /locus_tag="VHH53_03940"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX2289348.1"
                     /translation="MPTPIGGVAALAGARVLVAGARVTGVSAARALAELGAIVTITDS
                     SPAQLEALASSPAKSPGQLITGLTAVPRGTDLVVTSPGFRPDAPLMVAAHQAGVPVIG
                     DVTLARWIDRAHGDPADWLVVTGTNGKTTTVGMLESILHAAGIDAVACGNVGFPVLDA
                     VRAGHRVLAVELSSFQLHWSRELNPRAAAVLNVSADHLDWHGCMADYAADKGSVYGQD
                     TTAVFNVDDEWSTRLATGRGGVGFTTGEPRAGQLGIVGQQLVDRVFAQDTALAELADV
                     RPSGPHNLANALAAAALARSVGVPAQAVARGLRAFVPGSHRGAPVGAYAGVCYVDDSK
                     ATNPHAARAAVLSHQRVVWVAGGLLKGVDVDSLVIDTRDRLVGAVLFGADRAVIAAAL
                     ARHAPNLPVRMVDSGDDSAMIEVVAAAAELARPGDVVLLAPAAASMDMFTDYAQRGQA
                     FAAAVAALSEAPLHPGAR"
     gene            1985..>2228
                     /locus_tag="VHH53_03945"
     CDS             1985..>2228
                     /locus_tag="VHH53_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEX2289349.1"
                     /translation="MRKSPSRQETDTAAQWAAARAQLTAWLARPLTSLHLVLAVFGLL
                     TLFGLVMVLSASNVQSYTRVGSSYAVFVQQLAYCGVG"
BASE COUNT          285 a          728 c          803 g          412 t
ORIGIN      
        1 tgttcagcta cctgctggtc tcggcttggt cgaacgcggt gaacctcacc gatggcctgg
       61 atgggctggc agccgggtca gctgcgatgg tgctggtgac ctacgtcatc gtctcgttct
      121 ggcagttcac ccaggactgc accgttcagg ctgttcccgg ctgttacacg gtgcgtgacc
      181 cgctggatgt ggcagtggtg gctgccgccg cggtgggtgg gtgcatcggg tttctgtggt
      241 ggaatgcccc cccggcgaag atcttcatgg gtgataccgg ctcgctggcc atcgggggat
      301 tgctggccgg cctgtccgtg gtgacccgaa ccgaactgct tctcgtcgtg atcggtggac
      361 ttttcgtggt ggaggcgatg tcggtggtga tccaggtggt ggtgtttcgc accacccatc
      421 gccggccgtt tcggatggcg ccgtttcacc accacttcga gctggcgggg tgggccgaga
      481 ccactgtcat catccggttc tggctgctcg cggccatctg ctgtgccttg ggcgcaggag
      541 tgttctacag cgagtggctc aacgctgccg gcgggtgatc gatgcccaca ccgatcggcg
      601 gcgtcgccgc tctcgccggg gcccgggtgc tggtcgccgg ggctcgggtc actggagtct
      661 cggccgcgcg ggcgctggcc gagctaggtg ccatcgtcac catcaccgac tcgtcgccgg
      721 cccagctgga ggcgctggcc agctccccgg cgaaaagtcc aggtcagttg atcaccggcc
      781 tgaccgctgt gccccgtggc actgacctcg tggtgaccag tcctggtttc cggccggacg
      841 cgccgttgat ggtggccgcg caccaggccg gagtgccggt gatcggcgat gtgacgctgg
      901 ctcgctggat cgaccgtgcg cacggtgacc cagctgactg gctggtggtc accggcacca
      961 acggcaagac caccacggtg gggatgctcg agtcgatcct gcacgccgcc gggatcgacg
     1021 cggtggcgtg cggcaacgtc gggttcccgg tgctggacgc ggtgcgggcc ggccaccggg
     1081 tgctcgccgt ggagctgtcg tcgttccagc tgcactggtc gcgcgagctg aacccgcgag
     1141 ccgccgcggt gctcaatgtc tccgccgatc acctggactg gcatggctgc atggcagatt
     1201 acgcagcgga taagggcagc gtgtacggcc aggacaccac ggccgtgttc aacgtcgacg
     1261 acgagtggtc gacgcggctg gccaccgggc gtggcggggt cggcttcacc accggcgagc
     1321 cgcgggcagg tcagctgggg attgtgggcc aacagctggt cgaccgcgtg tttgcgcagg
     1381 acactgcgct ggccgaactc gccgacgttc gcccgagcgg tccacataac ctcgccaatg
     1441 cgctggccgc agcggcgttg gcccgttcgg tcggggtccc agcgcaggct gtcgcccggg
     1501 ggctgcgtgc ctttgttccg ggcagccacc gtggagcacc cgttggcgca tacgccggag
     1561 tttgctatgt cgatgattcg aaggccacca acccgcacgc ggcgcgggcc gccgtactgt
     1621 cccatcagcg ggtggtatgg gtggccgggg gcctgctcaa aggcgtcgac gtggactcac
     1681 tcgtcatcga cacccgggac cggctggtgg gcgcggtgct gttcggcgcg gatcgcgcgg
     1741 tgattgccgc cgcgctggcg cgacacgcac ccaacctccc agtgcggatg gtggattcgg
     1801 gagacgatag cgccatgatc gaggtggtcg cagccgccgc cgagctggcc cgcccgggtg
     1861 atgtcgtgct gcttgcaccc gcggcggcgt cgatggacat gttcaccgac tacgcccagc
     1921 gtggtcaggc attcgccgcc gcagtcgccg ccctcagcga ggcgccgttg catccggggg
     1981 cgcggtgaga aagagccctt cccgccagga gaccgacacc gcggctcagt gggcagcggc
     2041 ccgcgcccag ctcaccgctt ggctggcccg gccgctcacc tcactgcacc tggtgctcgc
     2101 cgttttcggc ctgctgaccc tcttcggctt ggtgatggtt ctctcggcgt ccaacgtgca
     2161 gtcctacacc cgggtggggt cgtcctacgc ggtgttcgtc cagcaactgg cgtactgcgg
     2221 cgtggggc
//
LOCUS       DATADN010000296         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1393702, whole genome shotgun sequence.
ACCESSION   DATADN010000296 DATADN010000000
VERSION     DATADN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393702"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VHH53_03950"
     CDS             complement(<1..564)
                     /locus_tag="VHH53_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2289350.1"
                     /translation="MTATGAPQTITLAKGLNQGLRVAMEADPKVIVMGEDVGKLGGVF
                     RITDGLQKDFGERRVLDTPLGESGIIGTAVGLALRGYRPVCEIQFDGFVFPAFDQLVS
                     QLARLHYRSQGKLRMPVVVRIPHGGGIGAVEHHSESPESYFAHTPGLKVVACANPVDA
                     YWMIQQAIDCDDPVIFFEPKRRYWDKAS"
     gene            complement(561..1718)
                     /gene="pdhA"
                     /locus_tag="VHH53_03955"
     CDS             complement(561..1718)
                     /gene="pdhA"
                     /locus_tag="VHH53_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX2289351.1"
                     /translation="MDVDGPQTAQQVIAGLRATSAGADLVQLLTPEGERVSHPQFDLQ
                     ISAAEIQSLYRDMVLVRRADREANALQRQGQLGLWAPLLGQEAAQIGAGRAMAPQDMG
                     FPSYREHGIAWCRGVDPTEVFGIFRGNDQGSWDPNSTGFQLYTIVIANQCLHATGYAM
                     GQRLDGKIGDAEHGEATICFFGDGATSQGDVHESFVWAAVYDAPVVFFCQNNQWAISA
                     GLDRQTRVPLYRRSAGYGFPGIRVDGNDVLATLAVTRWALRECRTGNGPVLIEACTYR
                     MGAHTTSDDPSRYRLATELEEWKLKDPIERVRVHLVREGLAEPEFFDGVAAESDELAA
                     RLRDYCTSMPVPDPERIFSKVYVEPPPYLEAQREEFLSYQASFVEIGDSDR"
     gene            complement(1875..>2686)
                     /locus_tag="VHH53_03960"
     CDS             complement(1875..>2686)
                     /locus_tag="VHH53_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289352.1"
                     /translation="CNALRLAHERAAMLEEVNRSRSGSDAQLDSNFGARVEKILMLAQ
                     DEAREVRRQADEAAAAVVEQARAEAAAQRQQAEQEAAAWRADMNRGAAEQDSALQRRS
                     AALDRARQELDRAREELERESERVRVQAQAEVEQMRKVVAQEADELFKAATQEADQVC
                     KAARVEAAELMAQARAEANRLVTTATEQAQQRERASAHELHQLSRLHEEINADLYRAK
                     EVLDSLFGGTRPALRATTGTGTGPSPIATSPPGTKRPRGDTQQDRRQARTV"
BASE COUNT          410 a          964 c          855 g          457 t
ORIGIN      
        1 tgacgccttg tcccagtacc gccgcttggg ctcgaaaaag atcaccggat cgtcgcagtc
       61 gatcgcctgc tggatcatcc agtaggcgtc gaccgggtta gcgcaggcga ccaccttgag
      121 tcccggagta tgagcgaagt aggactccgg ggactccgaa tgatgttcga cggcgccgat
      181 accgccgccg tgcgggatgc gcaccaccac cggcatccgc agcttgccct gcgagcggta
      241 gtgcagcctg gcgagctggg agacgagctg gtcgaaggcg gggaagacga acccgtcgaa
      301 ctggatctcg cagaccgggc ggtagccccg cagcgcgagc ccgacggcgg tgccgatgat
      361 ccctgactcg cccagtggag tgtccagaac ccgccgctca ccgaagtcct tctgcaggcc
      421 gtcggtgatc cggaacaccc cgcccagctt gccgacgtcc tcgcccatca cgatgacctt
      481 ggggtcggcc tccatcgcga ctcgcaggcc ctggttgagc cccttggcca gcgtgatcgt
      541 ctgcggtgcg ccggtagccg tcatcggtcc gaatccccga tctcgacgaa cgacgcctgg
      601 taggacaaga actcctcgcg ctgggcctca agatacggcg gcggttcgac gtagaccttc
      661 gagaagatcc gctccgggtc ggggaccggc attgacgtgc agtagtcacg cagccgtgcc
      721 gccagttcgt cggactcagc ggccacgccg tcgaagaact ccggctccgc cagcccttcc
      781 cgcaccaggt gcacccgcac ccgttcgatg gggtccttga gcttccattc ctccagctca
      841 gtggcgagcc ggtaacggct cgggtcgtcg gacgtggtgt gtgcgcccat ccggtacgtg
      901 catgcctcga tcagcaccgg accgttgccg gtgcggcatt cccgcagcgc ccagcgggtg
      961 acggccagcg tggcgagcac gtcgttaccg tccacccgga tcccgggaaa gccgtagccc
     1021 gccgaacgcc gatacagcgg cactcgggtc tgacgatcca gccctgccga gatcgcccac
     1081 tggttgttct ggcagaagaa caccaccggt gcgtcgtaga ccgcggccca gacaaagctt
     1141 tcatgcacgt caccctggga ggtggcgccg tcgccgaaga agcaaatcgt ggcctcaccg
     1201 tgctcggcgt caccgatctt gccgtccagc cgctggccca tcgcgtaacc ggtggcgtgc
     1261 aggcattgat tggcgatcac gatcgtgtac agctggaacc cggtgctgtt tggatcccag
     1321 gagccctggt cgttgccgcg gaagatgccg aacacctcgg tggggtccac tccgcgacac
     1381 caggcgatac cgtgctcgcg gtaggaggga aagcccatgt cctgcggggc catcgcgcgc
     1441 ccggcgccga tctgcgcggc ctcctgaccc agcagcggag cccacagacc gagctgaccc
     1501 tgccgctgca gggcgttggc ttcccgatcc gcccggcgca cgagcaccat gtcgcgatac
     1561 agcgactgga tctccgccgc ggagatctgg aggtcgaact gcgggtgcga gacccgctcg
     1621 ccctcagggg tgagcagctg caccaggtcc gcgcccgcgc tggtggcccg tagtccggcg
     1681 atcacctgct gcgcggtctg tggaccatcg acgtccatcg tggcatctcc ttgttgttgc
     1741 gctcccctcc tgtgagggac acgcggcgcc gccgccacag cgcggctccg ggacgtggat
     1801 cactgcctga agtcgacgct aacaaatcac tccatgcgca attctcgcac ggtaacccgg
     1861 acagagccgc tgcgtcacac cgtacgagcc tgacgacggt cctgctgcgt atctcccctg
     1921 ggtcgcttag tcccaggagg agacgtcgca atgggagagg gtccggtgcc cgtccccgtg
     1981 gtggcccgaa gagcgggcct ggtgccaccg aacaggctgt ccagcacctc cttcgcccgg
     2041 tagaggtcag cattgatctc ctcgtgcaac cgggacagtt gatgcagctc gtgggcactt
     2101 gctcgctccc gttgctgcgc ctgctcggtc gcggtggtga ccagccggtt cgcttcggcg
     2161 cgggcttgcg ccatgagctc cgcggcctcg acccgggccg ccttgcatac ctggtccgcc
     2221 tcctgcgtgg ctgctttgaa cagctcgtcg gcctcctggg caaccacctt gcgcatctgc
     2281 tcgacctcgg cctgcgcctg cacccggacc cgctcggatt cccgttccaa ctcctcacgc
     2341 gcccggtcca gctcttggcg agcccgatcc agcgccgcgg aacgccgctg caacgcgctg
     2401 tcctgctcgg cggccccccg gttcatgtca gctcgccagg cggcggcctc ctgctcggcc
     2461 tgctgccgct gcgcggcagc ctcggcacgg gcttgctcga ccaccgccgc agccgcttcg
     2521 tcggcctggc ggcgaacttc ccgtgcctcg tcctgggcca gcatgaggat cttctcgacc
     2581 cgcgcgccga aattcgagtc cagttgcgcg tcgctgccgg accgcgaacg attgacctct
     2641 tccagcatcg cggcccgctc gtgagccagc cgcagcgcgt tgcacg
//
LOCUS       DATADN010000297         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_943556, whole genome shotgun sequence.
ACCESSION   DATADN010000297 DATADN010000000
VERSION     DATADN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943556"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1064
                     /locus_tag="VHH53_03965"
     CDS             165..1064
                     /locus_tag="VHH53_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289353.1"
                     /translation="MSTVLNLHASGPRGVPPLLARLVDDAALFPPSATSLVDVVATHL
                     AARREPYAGVIGFLLCPISRLSALVTALRKAPPAQPVALSLVANTGLGGLPKAISTAL
                     DNGNLLDLRMIEVPAPSDVDSNWLVQLTEFVPDDVVRVVEPRRGRPEWLDGVRRVAEA
                     GCWPKLRCGGKSAESFPSIDEVADFLAIATGTGQPFKATAGLDHAVRHYDDCTGLTHH
                     GLLNMLVGTARTVSGGSVHEALRCTDATALAAEAGALSEECSRIVRNVFACYGSMSLT
                     DPVSDLIRLGLLPNPTSRQASQH"
     gene            1135..>1538
                     /locus_tag="VHH53_03970"
     CDS             1135..>1538
                     /locus_tag="VHH53_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEX2289354.1"
                     /translation="MAVTSYLGPEGTFAEQAAHVLAPGDELRSAVSVHAAIAALRAGE
                     VDGACVPVENSVEGSVPATLDAMAEGSPVLAVAETVLPIRFSVLVRPGTTAAGVRTVA
                     THPHAVAQVRDWIGAHLPEAATVLTPSTSAAAV"
BASE COUNT          203 a          529 c          531 g          275 t
ORIGIN      
        1 ggcctgggcg gatggtgctg gtggcgctgg caggcacgat cccgctgatg tcgttcgtgg
       61 ccgagcgcaa tgtgacccgt gtggtgcggg caagcctggc caactcgacg tcgccacggt
      121 gagccctggc gggcgccaac cagggcgcta ctaaggtgcg tcgagtgtca acggtgctga
      181 atctgcatgc ttccgggccg cgaggcgtcc caccacttct tgccaggctc gtcgatgacg
      241 cggcgctgtt cccgccctcg gcgacgagct tggtcgatgt cgtggcgacg caccttgctg
      301 ctcgccgcga gccgtacgcg ggggtgatcg gtttcctgct ctgcccgatc tcccggctca
      361 gcgctctggt cactgcgctg cgcaaggcac ctcccgccca accggtggcc ctgtcgctgg
      421 tggccaacac cgggctgggt ggcctgccca aggctatttc caccgcgctg gacaacggga
      481 acctgctgga cctgcggatg atcgaggtgc cggcaccatc ggacgtggac tccaattggc
      541 tggtgcagct caccgagttc gtgcccgatg acgtggttcg cgtggtggaa ccacgccgtg
      601 gccggccaga gtggctcgat ggggtacgcc gggtcgccga ggcgggctgc tggcccaagc
      661 tgcgctgtgg cggcaagtcc gcggagtcgt ttcccagcat cgatgaagtc gctgatttcc
      721 tggccatcgc caccggcacc ggccagccgt tcaaggccac cgccgggctg gaccatgcgg
      781 tccgccacta cgacgactgc accggcttga cccaccatgg gttgctcaac atgctcgtcg
      841 gcactgcccg cacggtatcc ggcggcagcg tgcacgaggc gctgcgatgt accgacgcta
      901 ccgcgctggc cgccgaggcc ggtgccctgt ccgaagagtg ctcgcggatc gtgcgcaacg
      961 tcttcgcctg ttacgggtcg atgtcgctga ccgatccggt gtccgatctg atccgcttgg
     1021 gactgctccc caaccccacg tcccggcaag cgtcccagca ctaacctcac ctcccgcgtg
     1081 tccgcacccg gtgcacggtg tgtccgactt tcaggtacct cgtaggctcg cgccatggcc
     1141 gtcacctcct acctcggacc cgaaggcacc ttcgccgagc aggccgctca cgtcctggcc
     1201 ccaggcgacg agttgcggtc cgcggtgtcg gtgcacgcgg cgattgccgc gttgcgggcc
     1261 ggagaggtgg acggtgcttg cgtgccggtg gagaactcgg tagagggttc ggtgccggcc
     1321 actctcgatg cgatggccga gggttcgccg gtgctggcgg tggctgaaac cgtgttgccg
     1381 atccggttca gcgtgctggt gcgtcccggt accaccgcgg cgggcgtgcg taccgtggct
     1441 acccacccgc acgcggtggc gcaggtccgg gattggatcg gcgcgcacct gcccgaggcg
     1501 gcgacggttc tgaccccctc cacctcggca gccgcggt
//
LOCUS       DATADN010000298         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_180957, whole genome shotgun sequence.
ACCESSION   DATADN010000298 DATADN010000000
VERSION     DATADN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180957"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..1721)
                     /locus_tag="VHH53_03975"
     CDS             complement(414..1721)
                     /locus_tag="VHH53_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2289355.1"
                     /translation="MTKKYQNDKIDTPAGDGFVVPERVSVAMAEIAGSMREGLLALAV
                     GTGLQVLQVLMEADVTALAGPKGRHDPARTAVRHGSERGSVTLGGRRVRVSRPRVRAA
                     DGSGELPVPTYELFGSTEILGSMAMERMLAGLSTRRYPVGLEPVGEQARAAASATSKS
                     AVSRKFVAMTETALAELLAADLSGLDLVALMVDGVHFGESVCIVALGVDEQGTKHPLA
                     LVEGSTENATVVTDLLTGLRARGLNVTRPILVGIDGAKALRKAVVEVFDHPVIQRCQL
                     HKIRNVQDRLPQRLRGPVGTKMRAAYHADSALDAEAQLGALAHELDKTHPGAAASLRE
                     GLAETLTVLRLGVPPTLARTLRSTNAIESMISICRAHSANVKRWRDGQMALRWCAAGM
                     VEAGKQFRRVNGHLHLAKLRAALDAEIAGNVAPAVQDEEVVAA"
BASE COUNT          301 a          747 c          697 g          332 t
ORIGIN      
        1 ggccatccgc ggtcgaccgc atcagccgtc tgacctggct gtttgtacga cggcgcacgt
       61 ccacgaaccc cgcacaaggg aactgtcagt tgcatggttt gagtcggctc cccagttggc
      121 caaccgatca tgaacggccc cccgacatgg cgatggtcga tggcgatcag gccgcaaacg
      181 cgtggtgcca cgatggtggc ctaaggtcgg cgttcagggt cattcgtgac ggttcgctga
      241 gaaccgcaca tgccgctgag ctgcgagaac ggactctgac gaacctgcgc caacccctat
      301 cagccgcatg gcagtgtggg ggtcaggggt tcagtttcct cagctccacc tcctgactca
      361 ggccgtgagg atgtcccgag ttccgtagaa cttcggcggc ggcccgttgt gcgtcaggct
      421 gcgacgacct cctcatcctg cacggcgggt gcgacgtttc cggcgatctc ggcgtcgagc
      481 gcggcgcgca gcttggccag gtgcaggtgg ccgttgacgc ggcggaactg cttgccggcc
      541 tcgaccatgc cggcggcgca ccagcgcagc gccatctgcc cgtcacgcca gcgcttgacg
      601 ttggccgagt gggcccggca gatggagatc atgctctcga tggcgttggt cgagcgcagc
      661 gtgcgggcca gcgtcggcgg cacgcccagc cgcagcacgg tgagggtctc ggccaggccc
      721 tcgcgcaggc tcgcggccgc gccggggtgg gtcttgtcca gctcgtgggc cagcgcgccc
      781 agctgggcct ccgcgtcgag agcggagtcg gcgtggtagg ccgcacgcat cttcgtgccg
      841 accgggccgc gcagccgctg cggtaagcgg tcctgaacgt tgcggatctt gtgcagctgg
      901 cagcgctgga tcaccggatg gtcgaacacc tcgacgacgg ccttgcgcag cgccttggcc
      961 ccgtcgatgc cgaccaggat cgggcgggtc acgttcagcc cccgcgcgcg caggccggtg
     1021 agcaggtcgg tgacgacggt ggcgttctcc gtcgagcctt cgaccagcgc cagcgggtgc
     1081 ttggtgccct gctcgtcgac gcccagcgcg acgatgcaca ccgactcgcc gaagtgcacc
     1141 ccgtcgacca tcagcgcgac cagatccaat ccggacaggt ccgcggccag cagctcggcc
     1201 agcgcggtct cggtcatcgc cacgaacttg cgcgagaccg cggacttgct cgtcgccgac
     1261 gctgcggccc tggcctgctc gccgaccggc tccaggccga ccggatagcg gcgggtggac
     1321 agcccggcca gcatccgctc catcgccatc gacccgagga tctcggtgct gccgaacagc
     1381 tcgtaggtgg gcacgggcag ctcgccggag ccgtcagcgg cgcgcacccg cggacggctc
     1441 acccgcaccc ggcgaccgcc gagcgtcacc gacccgcgct cggagccgtg ccggaccgcg
     1501 gtccgcgccg ggtcgtggcg gcccttcggc ccggccagcg cggtcacgtc ggcctccatc
     1561 agcacctgca gcacctgcag accggtgccg acggccagcg cgagcaggcc ctcgcgcatc
     1621 gagccggcga tctcggccat cgccacactc acccgctccg gcacgacgaa cccgtcgccg
     1681 gcaggcgtgt cgatcttgtc gttctggtac ttcttggtca cggcggtccc ctgttcgaag
     1741 cggtgtcttg gcggacgccc cacacctacc tcacggccgg tgcggacggg ggaccgccac
     1801 ctcaacttct acgagacccg ggacaacctc gccagtcgtt ggcgactttg attacggcta
     1861 ctcggtgggg gtcccgccac gtagcacttg gcacctccgc tggcacatgc gcctgaccta
     1921 cagcgatgcc ttatctggcc gtccgcgtac ggctgatgct ggtgagaaaa cctcagtttg
     1981 tggtccctgc cccggaaagg tgaaccgtga cctccttgga tgcctccgcg gccatctcgg
     2041 acgccgagcc gccacggttc cacccggacc tgccggt
//
LOCUS       DATADN010000299         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_341105, whole genome shotgun sequence.
ACCESSION   DATADN010000299 DATADN010000000
VERSION     DATADN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341105"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..505)
                     /locus_tag="VHH53_03980"
     CDS             complement(14..505)
                     /locus_tag="VHH53_03980"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX2289356.1"
                     /translation="MASRLQLRHRYPEPPERIHAVLTDPGYLRDKLRAVGGSGAELVS
                     REQDGRGITVVLRQAVPDDALPSFLRSAFSGGLTIRRTETWTNSGGSVHAMVDGAPGT
                     ITGTMHLEPDPAGCVLGAELTAEVPLPLIGSKVERIITDNIGKLMDAEYQFTLEWLRD
                     TGA"
     gene            complement(516..1292)
                     /locus_tag="VHH53_03985"
     CDS             complement(516..1292)
                     /locus_tag="VHH53_03985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2289357.1"
                     /translation="MGSERATAVVTGASSGIGAATARALAADGFHVVLGARRVQQCQQ
                     IADEIGGTALPLDVLDPDSVAAFAAAVPMARVLVNNAGGAKGLDPVLEANEDQWRWMW
                     EVNVLGTLRMTKALLPKLIDSGDGVLVTVTSIAALAAYDNGSGYTSAKHAQAAVHRTL
                     RGEHLGQPVRFIEIAPGMVETEFSVVRFGGDKQRADDVYAGLTPLSAYDVAEVIAFAV
                     SRPSHVNLDLIVIKPRAQHSAMRYARSLDADCDSVDARPR"
     gene            1421..>2435
                     /gene="mshA"
                     /locus_tag="VHH53_03990"
     CDS             1421..>2435
                     /gene="mshA"
                     /locus_tag="VHH53_03990"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEX2289358.1"
                     /translation="MICRIGTALRRVAVLSVHTSPLDQPGTGDAGGMNVYIVQTATRM
                     ASRGVEVEVFTRATSSGLPPVVELAPGVTVRHITAGPYEGLAKNDLPAQLCAFTAGVL
                     RAEARREPGYYDVVHSHYWLSGQVGWLARDRWGVPLVHTAHTLAKVKNASLAHGDTPE
                     PYTRVIGEQQVVAEADRLVANTPAETAQLIDLYGADPARTVTIPPGVDLERFTPGSRN
                     AARAELNLPLDAVVLAFIGRIQPLKAPDVLLRAAAEMLHRDPALRDRLRVLVVGGPSG
                     SGLAEPTVLHQLAAATGITEQVVFVPPQSGAELVSVYRAADVVAVPSYNESFGLVALE
                     AQAC"
BASE COUNT          380 a          834 c          828 g          393 t
ORIGIN      
        1 acggttggcc accctaggcc ccggtgtccc tcagccactc cagagtgaat tgatactcgg
       61 catccatcag cttgccgatg ttgtcggtga tgatccgctc gaccttgctg ccgatcaatg
      121 gcagcgggac ctccgcagtg agttcggcac cgagcacgca tccggcgggg tccggctcca
      181 ggtgcatcgt cccggtgatg gtccccggcg ccccgtcgac catcgcgtgc acgctgccgc
      241 cggagttggt ccaggtctcg gtgcggcgga tcgtcaaacc cccggaaaac gccgaacgca
      301 ggaaggacgg tagcgcgtcg tcgggcacag cttggcgcag cacaacggtg atgccgcgtc
      361 cgtcctgctc ccgtgacacc agctcggccc cggagccgcc taccgcacgc agcttgtcac
      421 gcagataccc cgggtcggtc agcaccgcgt ggatgcgctc aggcggctcg gggtagcggt
      481 gccgcagttg caatcggctg gccatggcac ggaggctacc gtgggcgcgc gtcgaccgag
      541 tcgcagtctg catccagact gcgggcgtaa cgcatagcgg agtgctgggc acgcggcttg
      601 atcacgatga gatcgaggtt gacgtgtgag gggcggctca cggcgaaggc gatgacctcg
      661 gcgacgtcgt acgcgctcag cggggtcagc ccggcataga cgtcatcggc ccgctgcttg
      721 tcaccgccga aacgcaccac ggagaactcg gtctccacca tgccgggagc gatttcgatg
      781 aaccgcactg gctgcccgag gtgctcaccg cgcaatgtgc ggtgcaccgc ggcttgcgcg
      841 tgtttggccg aggtgtagcc cgagccgttg tcgtaggccg ctaaagcggc gatcgaggtg
      901 accgtgacca gcacgccatc gccggagtcg atcaacttcg gcagcaaagc cttggtcatc
      961 cgtagggtgc cgagcacgtt gacctcccac atccagcgcc actggtcctc attggcctcc
     1021 aggaccggat cgagcccctt ggcgcccccg gcgttgttca ccagcacgcg tgccatgggt
     1081 accgccgcgg cgaaggcagc caccgagtcg ggatcgagga catccagcgg caatgcggtg
     1141 ccgccgatct cgtcagcgat ctgctggcac tgttgcaccc ggcgcgcgcc gagtaccacg
     1201 tgaaagccgt ccgcggccag tgctcgggcg gtcgccgccc cgataccgct gctggcaccg
     1261 gtcaccacgg ccgtcgcccg ctccgaaccc accccctcac cctaagcctc accctcgcat
     1321 gcagcgcatt cagcgggctc agcgtggcaa acaggatgct gataaccccg ctgagcccgc
     1381 tgaatgcggg taaggcaggg atgacgggca agatgggcga gtgatctgcc ggatcgggac
     1441 ggcgctgcgg cgtgttgcgg tgctctctgt gcacacctct ccgctggacc agccgggcac
     1501 cggcgacgcc ggtgggatga acgtctacat cgtgcagact gcgactcgga tggccagccg
     1561 tggtgttgag gtcgaggtct tcacccgggc cacctcgtcc gggctgcctc cggtggtgga
     1621 gctggcgccg ggggtcactg tgcggcacat caccgccggg ccctacgagg gtctggccaa
     1681 aaacgacctg cccgcccagc tgtgcgcctt caccgccggg gtgcttcgcg ccgaggcgcg
     1741 ccgcgagccg ggttactacg atgtggtgca ctcgcactat tggctatcgg gtcaggtcgg
     1801 ctggctggcc cgggaccgct ggggagtgcc gctggtgcat accgcgcaca ccctggccaa
     1861 ggtgaagaac gcctcgctgg cgcacggcga caccccggag ccgtacaccc gggtgatcgg
     1921 cgaacagcag gtggtggccg aggccgaccg gctggtggcg aacacccccg ccgaaaccgc
     1981 tcagctcatc gacctctacg gcgccgaccc ggctcgcacc gtcaccatcc cgcccggagt
     2041 cgatttggag cgcttcacgc cgggatctcg caatgccgct cgggcggaac tcaacctgcc
     2101 gctcgacgcg gtggtgctcg ccttcatcgg tcgtatccag ccgctcaagg caccggatgt
     2161 gctgttgcgg gccgcggccg agatgctgca ccgcgacccc gcgctgcggg accggctgcg
     2221 ggtgctggtc gtgggcggac cctcaggtag cgggctggcc gagcccaccg tgctgcacca
     2281 gctcgccgcc gcaacgggta tcactgagca ggtggtcttc gtcccaccgc aaagtggtgc
     2341 tgagctggtc agcgtgtacc gcgccgcgga tgtggtggcg gtgcccagct acaacgagtc
     2401 tttcggtctg gtggctctgg aagcgcaggc atgcg
//
LOCUS       DATADN010000300         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_635448, whole genome shotgun sequence.
ACCESSION   DATADN010000300 DATADN010000000
VERSION     DATADN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635448"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VHH53_03995"
     CDS             <1..641
                     /locus_tag="VHH53_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289359.1"
                     /translation="ATGVDERDGQSGQGGDGSARSRSLSWSPLSQSGAAGDEPGGGVI
                     IVRAYALLSDRNLHSWWQHSIGSVERVSTSCGVPAVEGALGTAPRGTRGALWGGALVA
                     VAADRVGADRLQPAGTAAPVPARTPRRRTPPGSRGPTGSDKGSRSSRFRNLRGTRRAP
                     WWPRLLQRAPALEDLDLAGCGGGGSRGVGDRFAAAVGGRRRPAGAELGTATR"
     gene            526..2628
                     /locus_tag="VHH53_04000"
     CDS             526..2628
                     /locus_tag="VHH53_04000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2289360.1"
                     /translation="MILLAVAVVVAGALVTGLLLPWVGGAGLLARNSVPPPGELPAEL
                     TGGVWGNSRVLAADGSVITTFYANDRVPVTTEQIASVMKRAMVAIEDTRFYEHSGLDV
                     RGTARALVTNVAAGSVQQGGSTLTQQLVKQTLAQTADTAEQRQAATEGSLGRKLREAR
                     LALALEEAYSKDEILTRYLNTVYFGNGAYGVQAAAQRYFSVDATDLTLPQAAMLAGLV
                     QSPANDNPLTDPAAARTRRNQVLQRMYELGYIDARRLAAASAQPVAVDPGPGSPNGCI
                     DATVGGFFCAYLEQYLQQQLGISRDILDNGGLTIQTTLRPDVQVAGDRAVLNQLPMGD
                     PLAGMYTAVEPGTGHVLAMSVNRRYGCDQPACESVVLNTAYSQGAGSTFKVFTAAAAL
                     ERGLPADYTLTPSNPYVSRVYTNNGRPYEVRAFANADVRTMTMEQALYLSSNTYFMAL
                     QDALGSVEGPVRVAERMGMHFTASSADQIIGGNRGSFTLGAEATSPLDLASAYATLAA
                     GGTRCDPTPVTAVLDRNGEPLTGPDGKPVGSGDNCTPAAVAPAVANTLAQMMRKDVEP
                     GYPGQTAAAAYVPGHQIAGKTGTTQNNFSIAFAGYTPQYSASVMVLNPKGNQDVGGQG
                     GGMGAGIWHDAMAPILTTAPMVPFLPADPVLAGSTVPANPPPAATTATQDTDGNTPTD
                     ENGNTPGTGTTRDNDIED"
BASE COUNT          433 a          966 c          928 g          354 t
ORIGIN      
        1 aggcgacggg cgtagatgag cgcgacggcc agtcgggcca gggcggtgat ggttcggctc
       61 ggtcgaggtc gctgagctgg tcgccgctga gccagtccgg tgcagctggc gatgaacccg
      121 gtggcggcgt gatcatcgtc cgggcgtacg cgctgttgtc ggatcggaac ctacacagct
      181 ggtggcagca cagcatcggc agcgtcgagc gggtcagcac ctcctgcggc gtcccggcgg
      241 tggagggtgc gctggggacg gcgccgcgcg ggacgcgggg agcgctgtgg gggggcgcac
      301 tggtggccgt ggcggccgac cgggtgggtg ctgaccggtt gcagccggcc ggcaccgcgg
      361 cacccgttcc agcaaggacg ccgcgtcggc gcacgccgcc aggttcgcgg gggccgaccg
      421 ggtcggacaa gggatcccgg tcctcccggt tccgaaacct ccggggaacc cgtcgggcac
      481 cgtggtggcc taggttgctc caacgtgcgc ccgcgctgga agaccttgat cttgctggct
      541 gtggcggtgg tggtagccgg ggcgttggtg accggtttgc tgctgccgtg ggtgggcggc
      601 gccggcctgc tggcgcggaa ctcggtaccg ccacccggtg agttgccggc cgagctgacc
      661 ggcggcgtgt ggggcaacag ccgggtgctg gcggccgacg ggtcggtgat caccaccttc
      721 tacgccaacg accgcgtgcc ggtgaccacc gagcagatcg cttcggtgat gaagcgggcc
      781 atggtcgcga tcgaggacac gcggttctac gagcacagcg gcctggacgt gcgggggacg
      841 gcacgcgcgc tggtcaccaa cgtggcggcg ggctcggtgc agcagggcgg ctcgacgctc
      901 acccaacagc tggtcaagca gaccctcgcg cagaccgccg acaccgccga gcagcggcag
      961 gcggccaccg aggggagcct cgggcgcaag ctgcgagagg cccggctggc gcttgccctg
     1021 gaggaggcct actccaagga cgagatcctc acccgctacc tgaacacggt gtacttcggc
     1081 aacggcgcct acggcgtcca ggccgcggcg cagcgctact tcagcgtgga tgccaccgac
     1141 ctcaccctgc cgcaggcagc gatgctggcc ggcctggtgc agagcccggc caacgacaac
     1201 ccgctgaccg accccgcggc cgcccgcacc cgccgcaacc aggtgctgca gcgaatgtac
     1261 gagctggggt acatcgacgc ccggcggctg gccgctgcct ccgcccagcc ggtggcggtc
     1321 gacccgggcc cgggatcccc caacggctgc atcgacgcca ccgtgggcgg cttcttctgc
     1381 gcctacctgg agcagtacct gcagcagcag ctcggaatca gccgggacat cctggacaac
     1441 ggcgggctga ccatccagac cacgctgcgc ccggatgtgc aggtcgccgg tgaccgggcg
     1501 gtgctcaacc agctccccat gggggacccg ctggccggca tgtacaccgc ggtggaaccc
     1561 ggcaccgggc acgtgctggc gatgagcgtc aaccgccgct acggctgcga ccagcccgcc
     1621 tgcgagtccg tggtgctcaa caccgcctac agccaaggtg ccgggtcgac cttcaaggtg
     1681 ttcaccgccg ccgccgccct ggaacggggc ctgcccgccg actacaccct gactccgagc
     1741 aacccctacg tctcccgggt ctacacgaac aacggccggc cctacgaggt gcgcgccttc
     1801 gccaatgccg acgtgcggac gatgaccatg gagcaggcgc tgtacctgtc ctccaatacc
     1861 tacttcatgg cgctgcagga cgcactcggc agcgtcgagg gcccggtgcg cgtcgccgag
     1921 cggatgggca tgcatttcac cgcgtcctcg gcggaccaga tcatcggcgg aaaccgcggc
     1981 tcgttcaccc tcggtgccga ggccaccagc ccattggatc tggccagtgc ctacgccacg
     2041 ctggccgccg gcggcacccg ctgcgacccg acgccggtca ccgcggtgct cgaccgcaac
     2101 ggcgaaccgc tgaccgggcc cgatgggaaa ccggtgggca gtggagacaa ctgcaccccc
     2161 gcagctgtcg cgccggcggt ggccaacacc ctcgcccaga tgatgcgcaa ggacgtcgag
     2221 ccgggctatc ccgggcagac cgccgcagcg gcctacgtcc ccggccacca gatcgcgggc
     2281 aagaccggaa cgacgcagaa caacttctcg atcgccttcg ccggctacac gccgcagtac
     2341 tccgccagcg tgatggtgct caaccccaag ggaaaccagg acgtcggcgg ccagggcggc
     2401 ggcatgggcg cgggcatctg gcacgacgcc atggcaccca tcctcaccac cgcaccaatg
     2461 gtccccttcc tccctgccga cccggtcctc gccggcagca ccgtcccggc caacccaccg
     2521 cccgcagcaa caaccgccac ccaagacacc gacggcaaca cacctaccga tgaaaatggc
     2581 aatacgcctg gcactggcac cacgcgcgac aacgacattg aggactaacg acctcgtgca
     2641 tggtcagcgg cgggccgtcc tcgacctgtt tagggccagt t
//
LOCUS       DATADN010000301         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_341656, whole genome shotgun sequence.
ACCESSION   DATADN010000301 DATADN010000000
VERSION     DATADN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341656"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1094
                     /locus_tag="VHH53_04005"
     CDS             96..1094
                     /locus_tag="VHH53_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEX2289361.1"
                     /translation="MEFRHLGRSGLLVSEIAYGNWITHGSQVEEEAATACVKAALDVG
                     ITTFDTADVYAGTRAEEVLGRALKGQRREGLEILTKVFWPTGPGPNDRSLSRKHIMES
                     ITGSLRRLQTDYVDVYQAHRYDYSTPLEETMSAFADIVHSGKAHYIGVSEWTASQIRA
                     GWELAQDLKIPLVSNQPQYSMLWRVIEAEVVPTSIELGVGQVVISPIAQGVLTGKYEP
                     GKQPPAGSRATDEKSGANFISRWMSDEVLGTVQRLKPLADQAGLSMAQLAVAWVLQNP
                     NVSAAIVGASRPEQVHDNAKASGVKLDADLMKAIDEIVEPVTERDAAKTVSPAQRP"
     gene            complement(1155..>1511)
                     /locus_tag="VHH53_04010"
     CDS             complement(1155..>1511)
                     /locus_tag="VHH53_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289362.1"
                     /translation="PDLVVQDPTGQWFLAGDPHTEFWAALGALAFLQVTASVLNFLPV
                     PGLDGGNMLHPWLSPPYQRAFNLFAPYGFILLFVLLWQSEINRWFFAAVYWLAELIGI
                     PEETAQLGMALMRFWS"
BASE COUNT          266 a          515 c          536 g          194 t
ORIGIN      
        1 tccggcggct gcggatgccc gcgccgcggg tgaagttcgg cgagcggccg gagggcgcga
       61 aaggctgacc cgcgccggtc attagggtcg gactcatgga attccgacac cttggccgca
      121 gcggcctgct ggtcagcgag atcgcctacg gcaactggat cacccacggc tcccaggtcg
      181 aggaggaggc cgccaccgcg tgcgtcaagg ccgctctcga cgtgggcatc accacgttcg
      241 acaccgcgga cgtgtacgcc ggcacccggg ccgaggaggt gctcggccgc gcgctcaagg
      301 gccagcgccg cgaagggctg gagatcctca ccaaggtgtt ctggccgacc ggaccgggcc
      361 cgaacgaccg cagcctgtcc cgcaagcaca tcatggagtc gatcaccggc tcgctgcgcc
      421 ggctgcagac cgactacgtg gacgtctacc aggcccaccg gtacgactac tcgacgccgc
      481 tcgaggagac gatgtccgcg ttcgccgaca tcgtgcactc cggaaaggcg cactacatcg
      541 gggtgtccga atggaccgcg tcgcagatcc gggcgggctg ggagctggca caggacctga
      601 agatcccgct ggtgtccaac cagccgcagt actcgatgct ctggcgggtc atcgaggccg
      661 aggtggtgcc cacctcgatc gagctgggcg tcggccaggt ggtcatctcc cccatcgcgc
      721 agggcgtgct gaccggcaag tacgagccgg gcaagcagcc gccggccggc agccgggcca
      781 ccgacgagaa gtccggtgcg aacttcatct cccgctggat gagcgacgag gtgctcggta
      841 cggtgcagcg cctcaagccg ctggccgacc aggccggcct gtccatggcg cagctcgcgg
      901 tcgcgtgggt gctgcagaac ccgaacgtct ccgcggccat cgtcggcgcg tcgcggccgg
      961 agcaggtgca cgacaacgcc aaggcttccg gcgtgaagct ggacgccgac ctgatgaagg
     1021 cgatcgacga gatcgtcgag ccggtcaccg agcgggacgc cgccaagacg gtcagcccgg
     1081 cgcagcgccc gtaacccggc tcccaccggc tatcacggga cgacatcccg cggagcgagg
     1141 cggagccggc aagctcagct ccagaaacgc atgagggcca tgccgagctg ggcggtttcc
     1201 tccgggatgc cgatcagctc ggccagccag tagaccgcgg cgaagaacca gcggttgatc
     1261 tcggactgcc agagcagcac gaacagcagg atgaagccgt acggcgcgaa caggttgaag
     1321 gcccgctggt acggcgggct cagccacggg tgcagcatgt tgccgccgtc caggccgggc
     1381 accggcagga agttcagcac gctcgcggtg acctgcagga acgccagtgc gcccagggcg
     1441 gcccagaact ccgtgtgcgg gtcgccggcc aggaaccact ggccggtcgg gtcctggacg
     1501 accaggtccg g
//
LOCUS       DATADN010000302         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_944402, whole genome shotgun sequence.
ACCESSION   DATADN010000302 DATADN010000000
VERSION     DATADN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944402"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VHH53_04015"
     CDS             complement(<1..1430)
                     /locus_tag="VHH53_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator"
                     /protein_id="HEX2289363.1"
                     /translation="MSTPTKTATEGHTRAERMRQPLTADVVTALADQHGVCVRPFTME
                     VGDPDTGELRYVPIPCGSTVESVCGPCARKARALRMAQCRQGWHLTEEPTFQADKPTQ
                     EQQAQLTERVDLVAQYCDAVHNGRVDDADDLREAIRDADDKLRQLGVRGHLPSPDLPA
                     KRPPKRSTSRRHDAPNLPTRKISKTTLGREFAGRYRPSMFVTLTCDSYGRVHQDGTPV
                     DPQRYDYQRAARDAVHFASLVDRWWQNLRRVVGWDVQYFATVEPQRRAAPHLHTAIRG
                     SIPHEVIRQVTAATYHQVWWPHHDQLIYSTDHLPVWDANRKTFVDPDTRQPLTPWDQA
                     LGQVQQPAHVVTFGHQVHSKGILGDTDDVGRHIGYLTKYLVKSVAEVVEPTTDAQHHH
                     ADRLHAELSVTPCSPRCAVWLLYGVQPQAVSSKTVPGHCKGRAHRRSTLGLPGRRVLV
                     SRKWSGKTLADHRADRKAFVAQALAAV"
     gene            complement(1364..>2024)
                     /locus_tag="VHH53_04020"
     CDS             complement(1364..>2024)
                     /locus_tag="VHH53_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289364.1"
                     /translation="AVTGTDALAVLDELVEAMHARKAAFAGHVRTVPITREHPLELLE
                     FDELGALTRYTDRKTRDAITEKVALLTTQGRALGFTVRGYVQEPTKDTVPVRELFPRR
                     ICLRVSAKSHVGMVLGDQAYERGAWANRITESEAGVGYVFGEGIREPLRVRAGWVPDE
                     AIKQLETFVTGPISHDTTVLPFPPKPTGPTGSTRGGAACPPPPRPRQKGTPGPSGCAN
                     H"
BASE COUNT          280 a          602 c          746 g          396 t
ORIGIN      
        1 accgcggcta gggcttgcgc gacgaatgct ttgcggtccg cgcggtggtc ggccagggtc
       61 ttgccggacc acttacgcga gaccagtacc cggcgccccg gcaggcccag ggtgctgcgg
      121 cggtgtgcgc ggcccttgca atgtccgggc accgtcttgg agctgacggc ctgaggttgg
      181 acgccataga gaagccacac cgcgcatcgc ggcgaacacg gggtcactga tagctcggcg
      241 tgtagacggt cggcgtggtg gtgttgcgcg tcggtggtgg gttcgaccac ttcggccacg
      301 cttttgacca aatatttggt caggtacccg atatgtcggc cgacgtcgtc ggtgtcgccg
      361 aggatgccct tggagtgaac ctggtggccg aaggtgacca cgtgcgctgg ctgctgtacc
      421 tggcccagtg cctggtccca tggggtgagg ggttggcggg tgtcggggtc gacaaaggtc
      481 ttgcggttgg catcccagac cggcaggtgg tctgtgctgt agatcagctg gtcatggtgg
      541 ggccaccaga cctggtggta ggtggccgcg gtgacctgcc ggatcacttc gtgcgggatg
      601 ctgccccgaa tcgccgtgtg cagatgcggc gccgcccggc gctggggttc gacggtagcg
      661 aagtactgaa cgtcccagcc gacgacgcgt cgtaagttct gccaccaccg atccaccagc
      721 gaagcgaagt gcaccgcatc ccgggcagcc cgctgatagt cgtagcgctg cgggtcgacg
      781 ggggttccgt cctggtgcac ccgcccgtag ctgtcgcagg tgagggtgac aaacatggat
      841 ggtcggtatc ggccggcgaa ctcccggccc agggtggtct tgctgatttt gcgggtgggc
      901 aagttcggcg cgtcgtgcct acggctagtg gagcgcttcg gtggacgctt ggccggtagg
      961 tccggtgagg gtaaatggcc ccggaccccg agttgccgaa gtttgtcgtc cgcgtcccgg
     1021 atggcctccc gtaggtcatc ggcgtcatcg acccgcccgt tgtgcaccgc gtcgcagtat
     1081 tgggcaacaa ggtctacccg ctcggtcagt tgtgcctgct gttcctgggt cggcttgtca
     1141 gcttgaaagg tgggttcttc ggtgaggtgc cagccttggc ggcattgcgc catccgcagt
     1201 gcccgggcct tgcgggcgca cggcccgcag accgactcga cggttgatcc acaaggaatg
     1261 ggcacatacc gcagttcgcc ggtgtccggg tcgccgactt ccatggtgaa gggccgcacg
     1321 cagaccccat gttgatcggc tagcgccgtc acgacatcgg cggtcagtgg ttggcgcatc
     1381 cgctcggccc gggtgtgccc ttctgtcgcg gtcttggtgg gggtggacat gccgcacctc
     1441 cccgagtcga tccggtcggg ccggtgggtt tgggggggaa gggcagcacc gtggtgtcgt
     1501 ggctgattgg gccggtgacg aacgtttcga gttgcttgat cgcctcgtcg ggcacccagc
     1561 cggcccggac gcgtaggggt tcgcggatgc cttcaccgaa gacgtagccg acaccggctt
     1621 cggattcggt gatccggttg gcccatgcac cgcgctcgta ggcctggtca ccgagcacca
     1681 tccccacgtg ggacttggcc gagacgcgca ggcagatccg ccgcgggaat agttcgcgga
     1741 ccgggacggt gtccttggtg ggttcttgga cgtaaccgcg caccgtgaac cccaacgccc
     1801 gtccctgcgt cgtgagtagg gcgacttttt cggtgatggc gtcgcgggtt ttgcggtcgg
     1861 tgtaccgggt gagtgcgccg agctcgtcga attccagcag ctccagtggg tgttcgcggg
     1921 tgatgggcac cgtccggacg tggccggcga aggcggcttt gcgggcgtgc attgcctcga
     1981 ccagctcgtc gagcacagcg agggcgtcgg tgccggtgac ggcg
//
LOCUS       DATADN010000303         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_785673, whole genome shotgun sequence.
ACCESSION   DATADN010000303 DATADN010000000
VERSION     DATADN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785673"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VHH53_04025"
     CDS             <1..731
                     /locus_tag="VHH53_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2289365.1"
                     /translation="TTLFAPTGGSAAVAGFNVVRDAAEVRRRIGVTGQFASVDEDLTG
                     TQNLVLLGRLLGAGRREASQRAAELLELFGLAEAANRPAQGYSGGMRRKLDLASSLVG
                     RPDVVFLDEPTTGLDPESRLGMWDVVRSLVAGGTTVLLTTQYLDEADRLADTITVLAQ
                     GKVVAAGTAAQLKDRVGRRTVSATLSAAADIGPALGTLRRAGLEPLQSGRTLTAAVGS
                     SRALAVVVRALDDAGIEADELGLH"
     gene            776..1591
                     /locus_tag="VHH53_04030"
     CDS             776..1591
                     /locus_tag="VHH53_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289366.1"
                     /translation="MTALATKPSTAQVRPAGSGVFTQVQVLTGRALRRLRDPRMIVLS
                     LLQPLIMLTLFSQVFQSIANSPGFPSGVSYIDYVLPAILVTTASQAAMWSGAGLATDL
                     RNGALARFRTLPISMTSVLVGRSVFDLVRSAGQLIVLLVAGAMLFGYAPAGGIPGTTL
                     ALLIGLAVGMGLSWVFIALASWMRNVELMQMVGFVVIFPLMFASSAFVPIAELPPWMR
                     MIAVINPLSYAIDAARGWALAQPALGPTIGALLTAAALTVAAAAIASRGVRRP"
BASE COUNT          229 a          586 c          574 g          290 t
ORIGIN      
        1 tcaccacgct gttcgccccg actggtggta gcgcggcggt ggccggtttc aacgtggtgc
       61 gcgacgctgc cgaggtgcgc cgccggatcg gtgtcaccgg ccagttcgcc tcggtggacg
      121 aggacctcac cggcacccag aacctggtgc tactgggccg gctgctgggt gctggccggc
      181 gcgaggcgag tcagcgggcc gccgagctcc ttgaactgtt cggactcgcc gaggccgcca
      241 atcggccggc ccagggttac tccggcggca tgcgacgcaa actcgacctg gccagcagct
      301 tggtcgggcg acccgatgtg gtgttcctcg acgagcccac caccggtttg gacccggaga
      361 gccggctggg catgtgggac gtcgtgcgta gcttggtggc cggcggcacc acggtgctgc
      421 tcaccaccca gtacctcgac gaggccgacc ggttggccga caccatcacc gtgctggccc
      481 aggggaaggt ggttgcggcg ggcaccgcgg cgcagctcaa agaccgcgtc ggccggcgca
      541 ccgtcagcgc gacgctttct gctgcagctg acatcggccc cgcgctaggt accctgcgga
      601 gagcagggtt ggagccgttg cagtccggcc ggacactgac cgctgcggtc gggtcctccc
      661 gcgcgctggc agtcgtggtg cgcgcgttgg atgacgcagg catcgaggcc gacgaactcg
      721 gcctgcacta accgacgctc gacgatgtct atctcacgct caccggagcc cggccatgac
      781 cgcgctcgcc acgaaaccca gcaccgcgca ggtccgcccg gccggtagcg gggtgttcac
      841 ccaggtgcag gtactcaccg gccgggcgct gcgccggctg cgggacccgc ggatgatcgt
      901 gctaagcctg ctccaaccgc tgatcatgct gaccctgttc agccaggtgt tccaaagcat
      961 cgcgaactca cccggctttc cgtccggggt gagctacatc gactacgtgc tgcccgcgat
     1021 cctggtgacc accgcaagcc aggcggcgat gtggtccggt gccgggctgg ccaccgacct
     1081 gcgcaacggt gcactggccc ggtttcgcac cttgcccatc tcgatgacct cggtgctggt
     1141 cggacgcagc gtattcgatt tggtgcgcag cgccggccag ctcatcgtgc tcctcgtcgc
     1201 cggcgccatg ctgtttggtt acgccccagc cggcgggatc cccggcacca cgctggcgtt
     1261 actgatcggg ctggccgtcg gcatggggtt gtcctgggtg ttcatcgcgc tggccagctg
     1321 gatgcgcaac gtcgagctga tgcagatggt cgggttcgtg gtgatctttc cgctgatgtt
     1381 cgcgtccagc gcgttcgtgc ccattgccga gctgccgccg tggatgcgga tgatcgcggt
     1441 catcaacccg ctcagctacg cgatcgacgc cgcccgggga tgggcgctgg cccagccggc
     1501 cttggggccg accatcggtg cgctgctcac cgctgccgcc ctcactgtcg cagccgccgc
     1561 catcgcctcc cgaggcgtgc gccgtccctg accccggtta ccgcagtctg tctccagacg
     1621 caccttccgg gaagccggaa accgcagtct ggctccagaa tgcggctagg gtcgttggg
//
LOCUS       DATADN010000304         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1394743, whole genome shotgun sequence.
ACCESSION   DATADN010000304 DATADN010000000
VERSION     DATADN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394743"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..>2461)
                     /locus_tag="VHH53_04035"
     CDS             complement(253..>2461)
                     /locus_tag="VHH53_04035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX2289367.1"
                     /translation="KSISPLGPAMTPSMVEAMKYVNLRMNSPHPAISAATPVLRYVMW
                     FSPVSFYGRSSLLGGAVNRSRLLVGVLVALVWVVAACASTPPPPGSGLLLPGYDRQAR
                     AQDDLFQFANGEWLRTTDIPGDRSQYSAFVALADQSETNLRAIVEDSAARRGAAGSPD
                     QQIGDFYASFMDTARLEALGAAPLKESLDRIDALASPTDLVRYFADSTAKAVPSPIGL
                     YVSQDAKNATAYIPGVSQDGLTLPDRDYYLKSDPQFVTIREQLRVYAARVLGLAGVAD
                     PEGAASRVLAMENRLAEVQWSAAQERDATATYNKFTVADATAQTPGLDWKIYFDAAGV
                     NTPDFIIAEPSYFTALGGALTTVALEEWKPYLKFKLIDSLAAYLSDAFVTARFDFRGR
                     DLAGQQENRPRWERGVATVDGALGDLLGQRYVQRHFPPDAKQQIDALVSKLIDAFRSS
                     IDGLDWMGDATKAQARDKLSKLAVNIGYPEKWKDYSKLRVARDDLVGNMVRSAQLEHQ
                     REIDRLGTPVDREEWFTTPQTVNAFYSAPRNEIVFPAAILQPPFFDATADDAVNYGAI
                     GGVIGHEISHAFDDQGRKFDGTGNLRDWWTPQDVQRFTERTTALVAQYAAYEPLPGEH
                     INGELTLGENIADLSGLTVAMRAYRLSLGDRPAAVMDGFTGEQRFFLGWAQVWRGKSR
                     DKALRVQLLSDPHSPHEFRTNGVVSNLPEYYGAFGVKDGDRLFRPPDQRVKIW"
BASE COUNT          411 a          767 c          821 g          462 t
ORIGIN      
        1 atgagcggtt gctttacgga cgtcaagccc ggctgcgctg ttggtaatgg agcggtaccg
       61 cgccccgtcc ccgggaagaa ctcgccgagg cagtgtggcc gtacggacgg ccgattcggt
      121 acctaccagc tccatctgcc agccgacgtg gtggtcgatg tcgagtgtgc cgtcagactc
      181 atggtgttgg gcagtcagcc gggcccagct gttcagctag ctgcgctgct gtgggacacg
      241 gggcgctgag catcaccaga tcttgacgcg ctggtctggt gggcggaaca gtctgtcgcc
      301 gtccttcacg ccgaaggcgc cgtagtattc cggcaggttg ctgacgacgc cgttggtgcg
      361 gaactcatgt gggctgtggg gatcgctcaa cagttgcacg cgcagcgcct tgtctcggga
      421 cttgccgcgc cacacctgtg cccatcccag gaaaaaccgt tgctcgccgg tgaaaccgtc
      481 catgacggcg gccggccgat cgccgagaga cagccggtac gcgcgcatcg ccacggtgag
      541 gccggacagg tcggcgatgt tctcgcctag cgtcaactcg ccgttgatgt gctccccggg
      601 caacggctcg taggccgcat actgtgcgac cagagcagtg gtccgttcgg tgaaccgttg
      661 cacgtcctgc ggcgtccacc agtcgcggag gttgccggtt ccatcgaact tgcggccctg
      721 gtcgtcgaac gcgtggctga tctcgtggcc gatcaccccg ccgattgcgc cgtagttcac
      781 ggcatcgtca gcggtcgcgt cgaagaacgg cggctgcagg atcgcggctg gaaacacgat
      841 ctcgttgcgc ggtgcgctgt agaaggcgtt gaccgtctgc ggcgtggtga accattcctc
      901 acggtccacc ggtgtgccga gccggtcgat ctcccgctgg tgttcgagct gggctgagcg
      961 caccatgttg cccaccagat cgtcgcgagc aaccctcagc ttcgagtagt ccttccactt
     1021 ttccggatag ccgatgttga cggccagctt gctgagttta tcccgagcct gggctttggt
     1081 tgcgtcgccc atccagtcca agccgtcgat ggacgagcgg aaggcgtcga tcagcttgct
     1141 gaccagagcg tcgatttgct gtttggcatc cggcgggaaa tgccgctgca cgtatcgctg
     1201 gccgagtaga tcacccagcg cgccgtcgac cgtggcgaca cctcgctccc agcgcggccg
     1261 attctcctgc tgcccagcca gatcccggcc gcggaagtcg aagcgtgccg tgacgaaggc
     1321 atcgctgagg taggcagcga ggctgtcgat gagcttgaac ttcaggtacg gcttccactc
     1381 ctccagcgcc acggtggtca gcgcgccgcc cagcgccgtg aagtagctgg gctcggcgat
     1441 gatgaaatca ggggtattca cccccgccgc gtcgaaatag attttccagt ccaatcctgg
     1501 agtctgcgca gtggcatcgg cgacggtgaa cttgttgtag gtcgccgtcg cgtcgcgttc
     1561 ctgcgcagcg gaccactgga cctcggcgag ccggttctcc atggcaagca cccggctggc
     1621 cgcgccttcc ggatcggcga caccggccag acccagtacc cgggcagcgt agacccgcaa
     1681 ctgctcgcga atcgtcacga actgtggatc ggacttcagg tagtagtcgc ggtcgggcaa
     1741 ggtgagcccg tcctgcgaga cgcctggtat ataggccgtc gcatttttgg cgtcctggct
     1801 gacatacagc ccgatgggac tgggaactgc cttggctgtg ctgtcggcga agtaccggac
     1861 gaggtcggtc ggggaggcga gcgcgtcgat gcggtccagt gactccttga gcggggcggc
     1921 tccgagcgcc tcgaggcgtg cggtgtccat gaagctcgcg tagaagtcac cgatctgctg
     1981 gtctggcgag ccggccgcgc cgcggcgggc cgcgctgtcc tcgacgatcg ctcgcaagtt
     2041 cgtctcggac tggtcggcaa gcgcgacgaa tgcgctgtac tgggatcggt cgcccggtat
     2101 gtcggtagtg cgaagccact cgccgttcgc gaactggaag aggtcgtcct gcgctcgcgc
     2161 ctggcggtcg tatccgggta gcagcagtcc tgaccccggc ggcggtggtg tgcttgcaca
     2221 ggcggctacc acccacacca atgccacgag cacgccaacc agcagccgag atcggttcac
     2281 agcaccccca aggagagaag atcgaccata gaacgagacg ggtgagaacc acatgacgta
     2341 acggagcacc ggcgtggcgg cactgatcgc tgggtgagga gaattcatgc gaaggttgac
     2401 atacttcatc gcctcgacca tcgacggtgt catcgctggg cccagcggtg agatcgattt
     2461 c
//
LOCUS       DATADN010000305         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_490405, whole genome shotgun sequence.
ACCESSION   DATADN010000305 DATADN010000000
VERSION     DATADN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490405"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VHH53_04040"
     CDS             <1..1073
                     /locus_tag="VHH53_04040"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX2289368.1"
                     /translation="PDLLCVFVGGNDPNAIELAGTHAMEVAGARTTLGCSAAGVIGAG
                     RGVEIEPAISVFAACFPGVRCTPLHLELVRTPDGAAITGMPDRREDDAVAVLLADPYT
                     FPIAAFVQRSNDDLAGLPLIGGVASGPRGGQSARLFLDGHSVPTGAVGVVLGGPVAAV
                     PVVSQGCRPFGPPMIVTKAEGNVLLELAGTPALTKLEQIVAELPPEERQAVARGLHIG
                     VAMDEYVEEHERGDFLIRGVMGADSERGAIAIAEVVEVGRTVRFQVRDAAAADDDLNE
                     SFSRFRAENGPVGGALLFSCNGRGKTMFPSADHDVLAVRAGLDTTGVAGFFAAGEIGP
                     VAGRNHLHGFTASILVFGAGAS"
     gene            complement(1090..>1988)
                     /locus_tag="VHH53_04045"
     CDS             complement(1090..>1988)
                     /locus_tag="VHH53_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005442207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX2289369.1"
                     /translation="VTERVDGLQDLGQDRHHHRTTDRPEFAFRKATKMVDGVAVTGWF
                     AEDFTLTELKTLRAVERLPQNRQRNTLFSGRYEIPTFQEVLDLRAQLSDELRRTLGVY
                     PETKHPTYFQQAVLPLEPRLLEILRLNRLNRPDAPVFVQSFEVTNLKQLREAGLRTNA
                     VQLLSASGAPFDTTAVGSGPTYADLSTQDGLRGIARYAQGIGPEKLQVIPRKVDGTLS
                     SPTALVTDAHQAALVVHPYTFRAENVFLPVGYQTSTVPTDYGRAVDEQTTYLQAGIDG
                     LFTDHPDIGVVARAEFRATAPR"
BASE COUNT          299 a          637 c          707 g          345 t
ORIGIN      
        1 gccctgacct gctgtgcgtg ttcgtcggcg gcaatgaccc gaacgccatc gagttggcgg
       61 gcacccacgc gatggaggtg gccggggcac ggaccacgct gggctgcagc gccgctggtg
      121 tgatcggggc cggccgggga gtggagatcg agcccgcgat cagcgtgttc gccgcatgct
      181 ttcccggggt caggtgcacc ccgctgcacc tggaactggt gcgcaccccc gacggtgctg
      241 ccatcaccgg aatgccggac cggcgcgaag acgacgccgt cgcggtgctc ctcgccgacc
      301 cctatacctt tcccatcgcg gccttcgtgc agcgctccaa cgacgacctc gccggtctgc
      361 cactgatcgg cggcgtggcc agcgggcctc gcggcgggca gtccgcgcgg ctgtttctgg
      421 acgggcacag cgtgccgacc ggcgcggtgg gagtagtgct cggtggtccc gttgccgccg
      481 tcccggtggt cagccagggt tgccgcccct tcggcccccc gatgatcgtc accaaggccg
      541 agggcaacgt gctattggag ctggccggga cgccagctct tacgaaactc gagcagatcg
      601 ttgccgagct ccctcccgaa gagcggcaag ccgtcgcgcg cggattgcac atcggcgtcg
      661 cgatggacga gtacgtcgag gagcacgagc gcggtgactt cctcatccgc ggcgtcatgg
      721 gcgccgacag tgagcgcggc gcgatcgcca tcgcagaggt cgtggaggtg ggtcgcactg
      781 ttcgcttcca ggtgcgggac gccgccgccg ccgacgatga tctcaacgag tcgttctccc
      841 ggtttcgggc ggagaacggt ccggtgggcg gcgcgctgct gttttcctgc aacgggcggg
      901 gcaagaccat gttcccgagt gccgatcacg atgtgctggc ggtgcgggcc gggctcgaca
      961 ccaccggcgt ggctggcttc ttcgcagctg gcgagatcgg cccggtcgcc ggccggaacc
     1021 atctccacgg tttcaccgcg tcgatcctgg tattcggcgc aggcgcttca taactggcga
     1081 cgtgagcaat cagcgtggcg ccgtggcacg gaactcggcg cgggcaacga cgccgatgtc
     1141 gggatggtcg gtgaacagac cgtcaatccc ggcctgaagg taggtggtct gctcgtcgac
     1201 cgcgcgcccg taatcggtcg gcacggtaga cgtctggtag ccaaccggca ggaagacgtt
     1261 ctcggcacgg aaggtgtagg gatgcacgac cagagcggcc tgatgtgcgt cggtcaccag
     1321 cgcggtgggt gagctcagcg tgccgtcgac tttgcgcggg atgacctgta gcttttccgg
     1381 tccgattccc tgggcgtagc gcgcaatgcc gcgcaggccg tcctgggtgg acaggtcggc
     1441 ataggtcggg ccggagccga cagctgtggt gtcgaaaggc gcgccggacg ccgacagcag
     1501 ctgcaccgcg ttggtccgca agccggcctc gcgcagctgt ttgagattgg tcacctcgaa
     1561 ggactgtacg aacaccggtg catccggccg gttgagtcga ttgagtcgga ggatctcgag
     1621 cagccgaggc tccagtggca gaacggcctg ctggaagtac gtggggtgct tggtctccgg
     1681 gtaaacgccc aacgtccgcc gcaactcgtc ggacagctgg gcccgaagat ccagcacctc
     1741 ctggaatgtc gggatctcat accgcccgct gaacagcgtg ttgcgttgcc ggttctgcgg
     1801 cagccgctct accgcccgta gcgtcttgag ctccgtgagg gtgaagtcct cggcgaacca
     1861 cccggtgacg gccacgccgt ccaccatttt ggtggccttt cggaaagcga actcggggcg
     1921 gtcggtggtg cggtgatggt gtcgatcctg tcccagatcc tggagaccat cgacgcgctc
     1981 ggtcacct
//
LOCUS       DATADN010000306         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1395302, whole genome shotgun sequence.
ACCESSION   DATADN010000306 DATADN010000000
VERSION     DATADN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395302"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VHH53_04050"
     CDS             complement(<1..1638)
                     /locus_tag="VHH53_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2289370.1"
                     /translation="MGLYACGGGDDLCTGPFCFSPPTQPEPSSIEAGENPQEGLAGRD
                     LPQPINVLVKDAEGKPVPGVTVTFAVSSGGGSLYSETAKSGVNGLAQVSWRLGTELGP
                     QSLQARATNKDGGPLAGSPLELSVVAVAPQPARLVLRTTLPETAQNGVPLDPQPVIEV
                     YDADDQPLSGVEVTASVTGGATASGGTTATSNAEGLATFEGLALVGPQGPQTLVFTVA
                     TPGVEAATGGPIQLLPGNPASMTAQGPTSFEGTVNSPVSPGPSVLVRDQAGNPAPGVT
                     VSFAANRNASVSPEEATTNEQGIAQVSWTLGSTANTSYTLTARIESSPIEPVRFTAMA
                     RPGSAGRLRLVTQPSSPTASGAAFATQPVIQLEDQNGNPTPQAGVTVTAAISSGPGGS
                     LSNATATTDGSGRAAFSGLALTGQAGSYTLSFSAAGLTGVNSSPFTITAGAAGKLALV
                     TQPSTLALSRAQLVIQPVVQVQDAAGNPIRQSGIVVTASVTSPATTVTGEAATTDENG
                     RAQFSALTLSGIPGPRDVTFSAAGLQSVTARVTLLSVA"
BASE COUNT          258 a          540 c          581 g          298 t
ORIGIN      
        1 tgcgacgctc agcagcgtga ctcgcgcggt tacactctgc agcccggccg ccgagaaggt
       61 cacatccctt ggccccggaa tgccgctcag cgttaatgca ctgaattggg ctcggccatt
      121 ctcatcggtt gttgccgcct cgccggtgac tgtggtcgct ggagacgtca cggaggcggt
      181 tactactatg cccgattgcc ggatgggatt gccggccgca tcctgaacct gaaccaccgg
      241 ctggatgacc agctgagccc ggctgagcgc cagggtagat ggctgggtga ccagggcgag
      301 cttgccggca gctcctgccg tgatcgtgaa tgggctcgag ttgacaccgg tgagaccggc
      361 cgcgctgaac gacagagtgt agctgccggc ctggccggtt aatgccaggc cactgaacgc
      421 cgcccggccc gagccgtcag tcgtggcagt tgcattgctc agcgagccgc cgggaccgga
      481 ggagattgcg gcagtcaccg tcaccccggc ttgaggcgtg gggttgccgt tctggtcttc
      541 cagctggatc accggttggg tggcgaacgc ggcgccgctg gccgttggag acgacggctg
      601 ggtcacgagc cggagccgtc ccgcactccc gggtcgagcc atggcggtga accggaccgg
      661 ctcgatcggc gaagattcga tgcgcgccgt cagggtatag gaggtgttcg ccgtactgcc
      721 cagcgtccag ctcacctggg cgatgccctg ctcgttcgtg gtcgcctcct cgggagagac
      781 cgaggcgttg cgatttgcgg cgaagctgac ggttaccccg ggtgccggat ttccagcctg
      841 gtctcgcact agcacggagg gccccggtga gaccggagag ttgaccgttc cctcgaagct
      901 cgtcggtccc tgggcggtca tggatgcggg gttgccgggg agcagctgga tcggcccgcc
      961 ggtggcggcc tctaccccag gcgtggcgac agtgaagacc agagtctgag gcccctgggg
     1021 accaaccagc gcgagaccct caaaggtggc gagtccctct gcgttggagg tggcggtggt
     1081 cccgccgctc gctgtcgcac cgccggtaac cgaggcggtt acctcgacgc cggaaagagg
     1141 ctggtcgtcg gcgtcgtaca cctctatcac cggctgcgga tcgagaggaa ctccattctg
     1201 cgcggtctcg ggcagggtgg tccggagcac cagccgggcc ggctgcggcg cgaccgctac
     1261 taccgacagc tcgagagggg aaccggccag cgggcccccg tccttgttcg tggctctggc
     1321 ctggagactc tggggaccaa gctctgttcc cagcctccaa ctcacctgcg ccaggccgtt
     1381 tacgccggat ttcgcggtct cgctgtaaag gcttccgcca ccgctgctga cggcgaaagt
     1441 gacggtgact ccgggaaccg gtttaccctc cgcgtctttc accagaacat tgattggctg
     1501 gggcaggtcc cgaccggcta acccctcctg cgggttctcg cctgcttcga tgctgctggg
     1561 ttcgggctgg gtcggaggac tgaaacagaa cggcccggtg cacagatcgt ctccgccgcc
     1621 gcaggcatag agccccatag cggcaaatac tgccgtgagc gagccgagcc ttcggcc
//
LOCUS       DATADN010000307         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_342417, whole genome shotgun sequence.
ACCESSION   DATADN010000307 DATADN010000000
VERSION     DATADN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342417"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VHH53_04055"
     CDS             <1..268
                     /locus_tag="VHH53_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HEX2289371.1"
                     /translation="LALLVVDRFGGGIGGPAGWYTTGLALAMAIAAGVQRRRWGLGLA
                     LTLQVAMVAGWFAHPALGGLGIVFVLVWSYLLYVRWAVARRLKA"
     gene            349..759
                     /gene="ndk"
                     /locus_tag="VHH53_04060"
     CDS             349..759
                     /gene="ndk"
                     /locus_tag="VHH53_04060"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX2289372.1"
                     /translation="MSERTLVLVKPDGVSRGLVGEVIARIERKGLTLAALELRTVPLD
                     LAEQHYAEHAEKPFFGSLLEFITSGAVVAAVVEGERAVAAFRQLAGGTDPVDKAAPGS
                     IRGDFALETQYNLVHGSDSVDSAKREIALWFPEL"
     gene            859..>1754
                     /locus_tag="VHH53_04065"
     CDS             859..>1754
                     /locus_tag="VHH53_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2289373.1"
                     /translation="MSDNGAALVHARGLRKRFGTVEAVRGIDVDVRPAETFGFLGPNG
                     AGKSSAMRMIACVSPRSAGELTVLGMDPDTDGPRIRARLGIVPQQDNLDTELSVRENL
                     HIYGRYFGMSRVAVRAKAAELLDFAQLTDRAADPVDLLSGGMKRRLTIARSLVNDPEL
                     MLLDEPTTGLDPQARHLLWERLFQLKQQGVTLIITTHYMDEAEQLCDRLVVMNGGLIA
                     AEGSPAELIARYSTREVLELRFRPGDQHTWFDAVEGLADRIEKLPDRLLLYVADGEAA
                     LSAAHQRGVRPMSSLVRRSTLE"
BASE COUNT          268 a          530 c          633 g          323 t
ORIGIN      
        1 gctggccctg ctggtcgtcg accggttcgg cggcgggatc ggaggcccgg cgggctggta
       61 caccaccggc ttggcgctgg cgatggcgat cgcggctggt gtgcagcgtc ggcggtgggg
      121 cttgggactg gctttgacac tgcaggtggc gatggtcgcc ggctggttcg cgcaccccgc
      181 actgggtggg cttggcatcg tgttcgttct ggtgtggagc tacctgctgt acgtgcgttg
      241 ggcagtcgcg cggcggttga aagcctgacc gcgctgggtc gaggctgcgt cggcgtcgtt
      301 aggctgccgc cggtacccgg ttccccagca agaggagaat gagcagcagt gagcgagcgc
      361 accctggtcc tggtcaagcc cgacggtgtc agtcgcggcc tggtcggtga ggtgatcgcc
      421 aggatcgagc gcaaggggct gacgctggcc gcgttggagt tgcgcacagt tccgcttgac
      481 ctggccgagc agcactacgc cgagcacgcg gagaagccgt tcttcggctc gctgctggag
      541 ttcatcacct ctggtgccgt ggtggccgcc gtggtcgagg gcgagcgggc agtcgcggcc
      601 ttccggcagc tggccggtgg aacggacccg gtggacaagg ccgctcccgg ctcgatccgc
      661 ggtgacttcg cgttggagac tcagtacaac ctggtgcacg gctcggactc ggtggattcc
      721 gccaagcgcg agatcgcatt gtggttcccg gagctgtaga ggatatccac agctctacgg
      781 tgcatggagc cgtaggtatg ccggtcgcgg ccgaaaacct gccgagatct gtcgggggcg
      841 ccgggtagcg tcgatcgcgt gtccgataac ggtgccgctc tcgtgcacgc ccgagggttg
      901 cgcaagcgat tcggcacggt cgaagctgtg cgcggcatcg acgtcgatgt ccgacccgct
      961 gagacgttcg gttttctggg tcccaacggt gcgggcaagt cctctgccat gcgcatgatc
     1021 gcttgcgtgt ctccgcgcag tgccggtgag ctcacagtgc tggggatgga cccggacact
     1081 gacggaccgc ggattcgcgc ccggctcggg atagtgccgc aacaggacaa cttggatacc
     1141 gagctgtcgg tgcgggagaa cctccatatc tatgggcgct atttcggcat gtcccgggtc
     1201 gcggtgcggg ccaaagccgc cgagttgctg gacttcgcgc agctcacaga ccgagcggcc
     1261 gatccggtcg atctgctgtc cgggggcatg aagcgccggc tgaccatcgc gcgctccctg
     1321 gtcaacgacc cggagctgat gctgctcgac gagccgacca ccggattgga cccgcaagcc
     1381 cggcacctgc tgtgggagcg gttgttccag ctcaagcaac aaggcgtcac gctgatcatc
     1441 acgacgcact acatggacga ggccgagcag ctgtgcgacc ggctcgtggt gatgaacggc
     1501 gggctgatcg ctgcggaagg ctcaccggct gagctgatcg ctcggtactc cactcgggag
     1561 gtacttgagc tgcgattccg tcctggtgat caacacacct ggttcgatgc ggtagaaggc
     1621 ctagccgacc ggatcgagaa gctacctgac cggttgctgc tttacgtcgc cgatggtgag
     1681 gcagcgctgt ccgccgcgca ccagcgtggc gtgcgaccga tgtcaagcct ggtccgacga
     1741 tccaccctgg agga
//
LOCUS       DATADN010000308         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1395419, whole genome shotgun sequence.
ACCESSION   DATADN010000308 DATADN010000000
VERSION     DATADN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395419"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            758..1891
                     /gene="rho"
                     /locus_tag="VHH53_04070"
                     /pseudo
     CDS             758..1891
                     /gene="rho"
                     /locus_tag="VHH53_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
BASE COUNT          406 a          734 c          608 g          302 t
ORIGIN      
        1 cgccgttcag ctcaccggcg tgttggtccg gccccggtca ggtgcgacaa cgtgccaatc
       61 agtgatccgc aagcgtagct gtggggcgac taggcgctgg ccgccgccag ggcgatagtc
      121 gggaggtcat tgagcctccc cagcgctgct tgagcgcgat cagcggcatg tgcggacatt
      181 gcatgaaaac ggcccacccg taagtgatct acgggcgggc cgtgtcagcc agctcacgcg
      241 ctacgcggca tgagcggaca attgcgccgg acgatgcggg ctatgggact agccagcgtc
      301 gtaccggtct agccgcagga ccgcttcatc aggaagcgga tgtgttcagc cgattcgcgg
      361 tcacaccgca gggtagccac cctgtgcgtg cccgttgccc ggtcataaga cgcacctagg
      421 cacgtcaacg aggccgacgg ggaacgtggt tgcgcgccga tcatcgggtg gggtagcttt
      481 cggtggcgtt tccctcttgt ggatgcaggc agatgacgtg cccccacaag atctctcaag
      541 attcccttcc tcccgctgga cgtgcttcgt ggtgaagccg ttcctagcgc gaacgggatc
      601 ccctctgcgg cgtgcaccgg cgtgtcccgg cccagtcact gcacacccca ctgatcaatg
      661 agtccaggaa gaaaccccta tgtatgacaa cgatttcccc agccagcctg ccgacaacgg
      721 caggacccat aagtcgcatg aggacggttc cccacggccc gtcaccggga tcctcgacat
      781 cgtggacaac cgcgccttcg ttcgcaccga cagctacctg cccggacccc gcgacgcccc
      841 agtcccgctg ggactggtcc gcgaacacgg gctgcgccgc ggtgacacgg tcaccggcac
      901 agcccgcccg gccggcaggg acgaaagacc cacccccctg ctccaggtcg acaccatcaa
      961 cggacaacac ccgcgtcacg ccctgtcccg ggctgtgttc tccgagctgg tcccgctcta
     1021 cccgaacgag cggatgcgac tggagaccga accccacgcc ctgaccacgc gggtgaccga
     1081 tctgctcatg ccagtcggca agggccagcg ggccttgatc gtcgcaccac cgaaggccgg
     1141 caagacctcc gtgctgcagg ccatcgcgca cggtatagcc aaaaaccacc cggaatgcca
     1201 cctcatgctc gtccttgtcg gcgaacgccc ggaagaagtc accgacctgg cacgcgccgt
     1261 gcccggcgaa gtcatcgctg ccacattcga ccacccgccc cacgagcaca ccgcactcgc
     1321 cgagctggcc atcgaacgcg ccaaacgcct ggtcgaggtg gggcgagacg tggtggtgat
     1381 gctggactcg ctgacccggc taggccgcgc ctacaacctg gccgcgccag cgtccggacg
     1441 gatcctgtcc ggcggcgtgg actccaccac ccttgtgccg ccgaaacggt tcctcggcgc
     1501 ggcccgcaat atcgagaatg gcggctcgct gaccatattc gccacagcgc tagtggacac
     1561 cggctcggtc ggcgacacac tgatcttcga ggaatacaaa ggaaccagca acgccgaact
     1621 aagactggac cgcaagatcg caaataaacg cgtctaccct gccgtcgaca tagcccagtc
     1681 cagcacacgc aaggaagagc tactgctgac caccaaggaa atggtagcca cccgcgccct
     1741 gcgccgggcc ctcgacggcc gggacagcca acaggccatc gacatgctcc tcgacggcct
     1801 ccgcaagacc cccaccaaca ccgagttcct cacccagctc accaggacta ccccgcataa
     1861 cggcagcggt tcggggactg ccagccggtg accgcgccgc aaacccagtc caacccagcg
     1921 cccctcgacg agccttgcaa ccgacactcg gacgtttccg acagcaacac ccgctattcg
     1981 gcaatacagg gcggtcggcg tcaggtgcca gagacgacat agggggcgag gacatagtcg
     2041 gggtgctgtc
//
LOCUS       DATADN010000309         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_637069, whole genome shotgun sequence.
ACCESSION   DATADN010000309 DATADN010000000
VERSION     DATADN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637069"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..1834
                     /gene="purF"
                     /locus_tag="VHH53_04075"
     CDS             296..1834
                     /gene="purF"
                     /locus_tag="VHH53_04075"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX2289374.1"
                     /translation="MGNEAPRYDPDDPSDLDDPTDLHDTSAHDDQPREECGVFGVWAP
                     GEEVAKLTYFGLFALQHRGQEAAGMAVSDGSQVLVFKDLGLVSQVFDEQVLSSLSGHI
                     AVGHCRYSTTGATTWENAQPTFRTTVTGSGLALGHNGNLVNTAELLQRAGKVGAPTRN
                     GATTDSDLITGLLAAATVDGGIEEAAQRLLPQLRGAFSLVFCNEDALYAARDPHGVRP
                     LVLGRLDHGWVVASETAALDIVGANFVREVEPGELLAIDAAGLRSSRFANPEPKGCVF
                     EYVYLARPDTTIAGRGVHATRVEIGRRLAQEHPADADLVIPVPESGTPAAIGYAQGSG
                     IPYGSGLVKNSYVGRTFIQPSQTIRQLGIRLKLNPLRDVIRGKRLVVVDDSIVRGNTQ
                     RALVRMLREAGAIEVHVRIASPPVRWPCVYGIDFASRAELIANGADGGDVDAIRRGIG
                     ADSLGYVSLENLIAASEQPASRLCTACFDGRYPIPLPPNESIGKHLLEDVGQESHRPA
                     LRIG"
     gene            1936..>2780
                     /gene="purM"
                     /locus_tag="VHH53_04080"
     CDS             1936..>2780
                     /gene="purM"
                     /locus_tag="VHH53_04080"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX2289375.1"
                     /translation="MTETDSPQSTYAAAGVDITAGERAVAQLRPHAERASRPEVLGGI
                     GGFAGLFKPKLDRWREPVLASSTDGVGTKILVAQAMECHHTIGLDLVAMVVDDLVVCG
                     AEPLFLQDYIAVGKVVPAQIATIVKGIADGCQQAGCALLGGETAEHPGLMEAGAYDIS
                     GTGVGIVEAAKMLRPDRIRPGDVLVALGSSGLHSNGYSLARHVLLDIARMPLEGHVEE
                     FGHTLGEELLAPTRIYARDCLALTAETGVRMFAHITGGGLARNLERVLPDGVEAVVER
                     GSWTPA"
BASE COUNT          432 a          978 c          944 g          426 t
ORIGIN      
        1 ggccgcaagt cgggctcgcg gcgggcggcg ccgatccatg cgatcaaggc agcggctgga
       61 ggtccggagc ccgccttgcg acggtggcgg ggtgttggtc tgggcacaaa ttctccgcgc
      121 gtgatggact ggattacccc cggggtaact ggatactccg ctttggcacc gtccgaccac
      181 ccagagcacc cagctctacc ccatcggcct agcatgcgcg atcgagccgg gcgcgaacgc
      241 accggcagcc actagactcc gagtggtgcc ccgtcccgtc gacagggagt cgctggtggg
      301 caacgaagcg ccccgctatg acccggacga ccccagcgac ctcgacgacc ccaccgacct
      361 ccacgacacc agcgcacatg acgaccaacc acgcgaagag tgcggtgtct tcggtgtctg
      421 ggcccccggt gaggaggtcg ccaagctcac ctacttcggc ctgttcgccc tgcagcatcg
      481 tggccaggag gccgccggca tggcggtgtc cgacggctca caggttttgg tgttcaagga
      541 cctcggactg gtcagccagg tgttcgacga gcaggtgctg tcaagtctgt ccgggcatat
      601 cgccgtcgga cactgccggt actccaccac cggcgccact acctgggaga acgcccagcc
      661 cactttccgg accaccgtca ccggctcggg gttggcgcta gggcacaacg gcaacctggt
      721 gaacaccgcc gaattgctgc agcgagccgg taaggtcggc gcgcccaccc gcaacggcgc
      781 gaccacggat tcagatctga tcaccggcct gctcgctgct gccacggtgg acggcggcat
      841 cgaggaggct gcccagcgcc tgctgcccca actgcgggga gcgttttcgc tggtgttctg
      901 caacgaggac gcgctgtatg ccgcgcggga cccgcacggt gtccgcccgc tggtgctggg
      961 aaggctcgac catggctggg tggtcgccag cgagaccgcg gcgctggaca tcgtcggtgc
     1021 caacttcgtc cgtgaagtcg agccgggtga gctgctggct atcgatgcgg cagggttacg
     1081 cagctcgcgc ttcgccaacc ccgagcccaa gggctgcgtg ttcgagtacg tctacctggc
     1141 ccgcccggac accaccatcg ccgggcgcgg ggtgcacgcc acccgggtgg agatcggccg
     1201 gcggctggca caagagcatc cggccgatgc cgatctggtc atcccggtgc ccgagtcggg
     1261 taccccggcg gccatcggtt acgcccaagg ctcgggaatc ccctacggct cggggctggt
     1321 gaagaactcc tacgtcgggc gcaccttcat ccagccgtcg cagacgatcc gccagctcgg
     1381 catccggctc aagctcaacc cgctgcgcga cgtcatccgc ggcaagcgcc tcgtggtggt
     1441 cgacgactcc atcgtgcgag gtaacaccca gcgcgctctg gtccggatgt tgcgcgaggc
     1501 cggggcgatc gaagtccacg ttcggattgc cagcccaccg gtgcgctggc cgtgcgtcta
     1561 cggcatcgac ttcgcctccc gtgccgaact catcgccaac ggcgccgacg gcggcgacgt
     1621 cgatgcgata cggcgcggga tcggagctga cagtctgggc tatgtcagcc tcgagaacct
     1681 catcgccgct tccgagcagc cggcgtcgcg gctgtgcacc gcgtgcttcg acggtcgcta
     1741 cccgatcccg ctgccgccca acgagtccat cggtaagcac ctgctcgagg acgtcgggca
     1801 ggaatcacac cggccggcgt tgcggatcgg gtgagcagcg ccggggcatc gcgcccatac
     1861 ctgagcgagt agcgtgacct actgctcgta aggcatcttc gcgccaccag acccgtgtcc
     1921 tggagtccgc atgccatgac cgagaccgac agcccgcagt ccacctacgc cgcagccggg
     1981 gtggacatca ccgccggcga acgggccgtc gcccagctgc gcccgcacgc cgagcgggcc
     2041 agccgccccg aggtactggg cggcattggc ggcttcgctg gcctgttcaa gcccaagctc
     2101 gaccgctggc gtgaacccgt cctggcgagt tccactgacg gtgtagggac caagatcttg
     2161 gtggcgcagg cgatggaatg ccaccacacc atcgggctcg atctggtggc gatggtcgtt
     2221 gacgacctgg tggtctgcgg tgccgagccg ctgttcctgc aggactacat cgccgtcggc
     2281 aaggtcgtgc cggcgcagat cgccacgatc gtcaagggca tcgccgacgg ctgccagcag
     2341 gcgggctgcg cgctgctcgg cggcgagacc gccgagcacc ccggtctgat ggaggcgggg
     2401 gcctacgaca tctccggcac cggcgtcggg atcgtggagg ccgcgaagat gttgcggccc
     2461 gaccgcatcc ggccgggcga cgtgctcgtg gcgctcggct cgtccgggct gcactccaac
     2521 ggctactccc tcgcacggca cgtcctcctg gacatcgcgc ggatgccgct cgaaggtcat
     2581 gtcgaggagt tcgggcacac cctcggggag gagctgctcg cccccacccg gatctacgcg
     2641 cgcgactgcc tcgcgctcac cgcggagacc ggcgtgcgga tgttcgcgca catcaccggt
     2701 ggcgggctcg cccgcaacct ggagcgcgtg ctccccgacg gggtcgaggc cgtggtggag
     2761 cgcggcagct ggaccccggc
//
LOCUS       DATADN010000310         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_342934, whole genome shotgun sequence.
ACCESSION   DATADN010000310 DATADN010000000
VERSION     DATADN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342934"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..949
                     /locus_tag="VHH53_04085"
     CDS             2..949
                     /locus_tag="VHH53_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2289376.1"
                     /translation="MVLVEHAAGDIKKVTGELLTAAAKLGEPSAVVVGTPGTASKLKD
                     RLAAYGAVKIYVAESDEVDDYLTAPAVGVLAALVESARPIAVLIATSPDGKEIAGKLA
                     VRTGSGLLSDAVDLADDGTATHAVFGGSYLAKSKATTPVAVVTIRPGSIDAIEAQGAA
                     VEQAVEVPPVSPGKAAKIIERRPIGGGDRPELAEASVVVSGGRGVGSADNFQVVEKLA
                     DSLGAAVGASRAAVDSGYYPHQFQVGQAGKTVSPQLYVALGISGAIQHRAGMQTSKTI
                     VAVNKDPEAPIFEIADFGVVGDLFTIAPQLTEAVTKRKA"
     gene            1200..2402
                     /locus_tag="VHH53_04090"
     CDS             1200..2402
                     /locus_tag="VHH53_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX2289377.1"
                     /translation="MTYLDHAATTPMLPEAVAAMSQALSTLGNASSLHGSGRRARRTV
                     EESRESIADALGARPSEVVFTGGGTEGDNLAVKGMFWQRRAADPRRNRVLASAVENHA
                     VLDAVSWLAEHEGAEVVWLPVDADGRIHPETLRAALAGDPGSVALVSVMWANNEVGTA
                     APVQELAEVAAEYGVPLHTDAVQAVGQLPVHFGRSTTAAMTLTGHKLGGPYGVGVLLL
                     GRHVAATPLLHGGGQERDVRSGTLDVPAIVGLATAIRHAVAAQPRSAIRLGTLRDELV
                     DGIVAKVPDAVLNGDSGDGVIDGSPSRLPGNAHLTFPGCEGDSLLMLLDARGIECSTG
                     AACTAGVAQPSHVLLAMGASPAAARGSLRFSLGHTSTVQDVADVLDAIEPVVTRARGA
                     GIRRARAS"
     gene            2399..>2737
                     /locus_tag="VHH53_04095"
     CDS             2399..>2737
                     /locus_tag="VHH53_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX2289378.1"
                     /translation="MKVLAAMSGGVDSSVAAARAVDAGHEVVGVHLALSAQPGTLRTG
                     ARGCCTKEDAADARRVADNIDIPFYVWDFAQRFTADVVDDFVAEYAAGRTPNPCLRCN
                     ERIKFSAVLDR"
BASE COUNT          389 a          923 c          991 g          434 t
ORIGIN      
        1 cctggtgctc gtcgagcacg cagcgggcga catcaagaag gtcaccggtg agctgctcac
       61 tgccgcggcg aagctgggtg agccgtcagc ggtcgtggtc ggcacgcctg gcaccgcgag
      121 caagctcaag gacaggttag cggcatacgg tgcggtcaag atctacgttg cggagtccga
      181 cgaggtggac gactacctca ccgctcctgc tgtcggtgtg ctggcggcgc tcgtggagtc
      241 ggcccggccg atcgccgtgc tgatcgccac ctcgccggac ggcaaggaga tcgcaggcaa
      301 actcgccgta cgcaccgggt cgggcctgct ctccgatgcc gtcgacctgg ccgacgacgg
      361 caccgccacg catgcggtgt tcgggggctc ctacctggca aagtccaaag ccactacgcc
      421 ggttgcagtc gtcacaatcc ggcccggttc gatcgacgcg atcgaggccc agggcgcggc
      481 tgtcgaacag gctgtcgagg taccaccggt gagtccgggg aaagccgcga agatcattga
      541 gcgtaggcct atcgggggcg gcgaccgccc ggaactcgcc gaggccagcg tggtggtctc
      601 cggcggccgg ggtgtcggct cggcggacaa cttccaggta gtcgagaagc tcgccgattc
      661 gctcggtgcc gcggttggag cgtcccgggc ggcggtcgac tccggctact acccgcatca
      721 gttccaggtc gggcaggccg gcaagacggt ctctccgcag ctgtacgtcg cgcttggcat
      781 ttccggcgcc atccagcacc gcgcggggat gcagacatcc aagacgatcg tcgcggtcaa
      841 caaggacccg gaggcgccga tcttcgagat cgccgacttc ggtgtggtgg gcgacctgtt
      901 caccatcgcc ccgcagctga ccgaggcggt caccaagcgc aaagcctgac tccccgcgtg
      961 tccggccctc ctgcccggta tgttgcgctc tcttgctcgg tgtgtcagcc tctcctgcac
     1021 ggctggcggg tcttcgtgga ccgggatcgg tcggcgcctc gcacgccgcc atcactacgg
     1081 cgaccctgcc attggttgcg ctggcacctc cggtggtgcg ggaccggcgc acctgcgcga
     1141 cgtgcggcgt tgcggcccgc gccttcacca ccgcgggcgc cgtttacgct ggtgtctcga
     1201 tgacctacct cgaccacgcc gccacgacgc cgatgctgcc ggaggcggta gcagcgatga
     1261 gccaggcgtt gtccaccctg ggcaatgcct cgtcgttgca tggctccggc cgccgagctc
     1321 gtcgcaccgt cgaggaaagc cgggagagca tcgctgatgc gctgggtgcc cgcccctcgg
     1381 aagtcgtctt caccggaggc ggcacggagg gtgacaacct cgccgtcaag gggatgttct
     1441 ggcagcggcg tgcggccgac ccgcgccgca accgggtgct cgcttccgcc gtcgagaatc
     1501 acgctgtgct cgatgcggtg tcctggctgg ccgagcatga gggcgccgag gtggtgtggc
     1561 taccggtaga tgccgacggc cggatacacc ctgagacgct gcgggctgcg ctggccggcg
     1621 acccaggctc cgtcgcgctg gtgagcgtga tgtgggcgaa caacgaggtg ggcactgcgg
     1681 caccggtgca ggagttggcc gaggtcgccg ccgagtacgg ggtgccgctg cacaccgacg
     1741 cggtccaggc ggtgggccag ctgccggtgc atttcggcag gtccacaacc gcggcgatga
     1801 ccctgaccgg gcacaagctc ggcggaccct acggcgtcgg cgtgctgcta ctgggccggc
     1861 acgtcgcagc cacgccacta ctgcacggcg gcggccagga gcgtgatgtg cgttcgggca
     1921 cgttggacgt cccggcgatc gtcggcctgg ccaccgcgat ccgccacgcg gtagcggcgc
     1981 agccgcgcag cgcgatccgg ctcggtacgc tgcgtgacga actcgtggac ggcatcgtcg
     2041 ccaaggtgcc cgatgcggtg ctcaacgggg actcgggtga cggtgtgatc gacggcagcc
     2101 cctcccggtt gccgggcaat gcccatctga ccttccctgg ttgcgagggt gactcgctgc
     2161 tgatgctgct ggacgctcgg ggcatcgaat gctcgacggg agccgcctgc accgccggtg
     2221 tggcgcagcc ctcgcacgtg ctgctggcga tgggagcctc gcccgccgcc gcgcggggct
     2281 cgctgcgctt ctccctggga cacacctcga cggtgcagga cgtggctgat gtgctcgacg
     2341 cgatcgagcc ggtggtcacc cgggcccgtg gagcggggat ccggcgggcc agggcatcgt
     2401 gaaggttctg gctgcgatga gtggcggggt ggactcctcg gttgcggccg cccgcgcggt
     2461 ggacgccggg cacgaggtgg tcggcgtgca cttggcactg tcggcacagc ccggcacgct
     2521 gcgcaccggc gcccgcgggt gctgcactaa agaggacgcg gcggacgcca ggcgggtcgc
     2581 cgacaacatc gacatcccgt tctacgtctg ggacttcgcg cagcggttca ccgccgacgt
     2641 ggtggacgac ttcgtcgccg agtacgccgc aggccggacc cccaacccgt gcctgcgctg
     2701 caatgagcgg atcaagttct ccgcggtgct ggaccgg
//
LOCUS       DATADN010000311         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_343147, whole genome shotgun sequence.
ACCESSION   DATADN010000311 DATADN010000000
VERSION     DATADN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343147"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..91)
                     /locus_tag="VHH53_04100"
     tRNA            complement(16..91)
                     /locus_tag="VHH53_04100"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:His,seq:gtg)
     gene            complement(148..759)
                     /gene="orn"
                     /locus_tag="VHH53_04105"
     CDS             complement(148..759)
                     /gene="orn"
                     /locus_tag="VHH53_04105"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX2289379.1"
                     /translation="MNDRLVWIDCEMTGLDVVKDALIEIAALVTDGELRVLGEGIDVV
                     IHADDAALDSMPEVVREMHAKSGLTEAVRRSTVSLAEAEQQVLAYVRKWVPEARSAPL
                     AGNSVATDRAFLSRDMVELDEHLHYRMVDVSSIKELCRRWYPRVYYAQPAKGLANRAL
                     ADITESIRELAYYRRTVFVPEPGPNSEEAQAAAQAVLDETPGR"
     gene            complement(770..>1516)
                     /locus_tag="VHH53_04110"
     CDS             complement(770..>1516)
                     /locus_tag="VHH53_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2289380.1"
                     /translation="VEAWWGFRLVREPGGTLRIRRGLLTVRSVSLEERRLRGVEVVEP
                     LLLRAGRGARTGAVATGLGSGRGTLLPPAPRDEAHRVSGVVLTEDPSPTRTALRRHPR
                     VALRRRLARAVLPVVAASAVLRLAAVPTWVAWATLGLLPVTVLLAVDAYRNLGHALTS
                     RYLVTRHGAGMRGTVALQRAGVIGWTVSQSFFQRRAGLVTVTATTAAGSGAYRVIDVR
                     TADGLAVAERAVPGLLTPFLLQNVATPPAH"
BASE COUNT          249 a          576 c          486 g          205 t
ORIGIN      
        1 acagacgccc acagttgggg tgagtgacgg gattcgaacc cgcgacatcc tggaccacaa
       61 ccaggtgctc taccagctga gctacaccca ccatgcgctg acacctcgcc agctccgatc
      121 accatgatat ccggcgctgc ggcgcggtta ccgcccgggc gtctcatcga ggacggcctg
      181 agccgccgcc tgcgcctcct cggagttcgg gcccggttcg ggcacgaaca cggtgcgccg
      241 gtagtacgcc agctcgcgga tggactcggt gatgtcggcc agcgcccggt ttgccaggcc
      301 cttggccggc tgtgcgtagt agacccgcgg gtaccagcga cggcacagct ccttgatcga
      361 tgagacgtcg accatccggt agtgcaggtg ctcatcgagc tccaccatgt cgcggctcaa
      421 gaatgcccgg tcggtggcca ccgagttgcc ggccagcggc gccgagcgcg cctcgggcac
      481 ccactttcgc acataggcca gtacctgctg ctcggcctcg gcgaggctca ccgtggaccg
      541 ccgcaccgcc tcggtcaggc cggacttcgc gtgcatctcg cgcaccacct cgggcatcga
      601 gtccagcgcg gcatcatcgg catggatcac cacgtcgatg ccctcgccga gtacccgcaa
      661 ctctccgtcg gtgaccaacg cggcgatctc gatcagcgcg tccttaacca cgtccaagcc
      721 agtcatctcg cagtcgatcc acaccagacg gtcgttcaca cccgtgagcc tagtgcgccg
      781 gcggcgtcgc cacgttctga agcaggaaag gcgtgagcaa tccgggcacg gcgcgctcgg
      841 caacggccag cccgtcggcg gtccgaacat cgatcacgcg gtaggcaccg ctgcccgctg
      901 cggtcgtcgc ggtcacggtg accaacccgg ctcggcgttg aaaaaacgac tgcgacaccg
      961 tccagccgat cacccccgcg cgctgcagcg ccaccgtgcc gcgcatgccg gcgccgtgcc
     1021 gtgtgacgag atagcggctg gtcagcgcat gtccgagatt gcgataggca tctaccgcga
     1081 gcagtaccgt caccggcagc aggcccaggg ttgcccacgc cacccaggtg ggcaccgcag
     1141 ctagccgcag cactgcggat gccgctacca ccggcagcac cgcgcgggcc agccgccgtc
     1201 gcagcgcgac gcgcgggtgc ctgcgcaacg ccgtgcgggt aggggaggga tcctcggtga
     1261 gcacgacccc cgacacccgg tgcgcctcgt cccggggggc gggcggcagc agggtgcctc
     1321 gaccgctgcc tagcccggtg gccaccgcac cggtacgggc cccgcggccg gcccgcagca
     1381 gcagtggctc gaccacctcg acgccgcgca gccggcgctc ctccagcgac accgaccgca
     1441 cggtcaacag tccccggcgg atccgcagag tgccgcccgg ctcgcgcacc agccggaatc
     1501 cccaccaggc ctcaac
//
LOCUS       DATADN010000312         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1096343, whole genome shotgun sequence.
ACCESSION   DATADN010000312 DATADN010000000
VERSION     DATADN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096343"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VHH53_04115"
     CDS             complement(<1..507)
                     /locus_tag="VHH53_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2289381.1"
                     /translation="MASRSRTEASRLKEFASGLSFQLDPFQREACEALEDGHGVLVCA
                     PTGAGKTVIGEFAVHLALSQRRKCFYTTPIKALSNQKYADLVTRHGPHAVGLLTGDTS
                     INGNAPVVVMTTEVLRNMLYAGSTTLGGLAYVVMDEVHYLADRFRGAVWEEVILNLPG
                     SVRVVGLSA"
     gene            complement(517..1398)
                     /locus_tag="VHH53_04120"
     CDS             complement(517..1398)
                     /locus_tag="VHH53_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2289382.1"
                     /translation="MPVTLIVNPHSGRGATVRVAGTVAATLRAAVPELTATVTADAAA
                     TSRLAAQAAQRGDDVLAVLGGDGTVHLAAQAVAGTQTALAVLPSGTGNDLATALGIPT
                     GPVHAARHVADALRSGQRRRIDLGRVEGGACYTTVLCTGFDAAVNARANAMRWPAGPR
                     RYDLAVLAELAALTPRPVQVHAGDSKMDLEAILVAVGNGPSYGGGLRICPDATLDDGL
                     LDVIAITAVNRRRLLRVFPSLRTGAHVDEPEVTVLRAATVRIDGDPRWPVYADGEPQG
                     TLPVTMHCQPAALTVVA"
     gene            complement(1408..>1622)
                     /locus_tag="VHH53_04125"
     CDS             complement(1408..>1622)
                     /locus_tag="VHH53_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX2289383.1"
                     /translation="PISMLVLAGALVVLFEASVLIARAHDRTLARRRAEQGWDDLDPD
                     EPSPLDHRVEPVASEGTTSPRYDDAT"
BASE COUNT          232 a          583 c          574 g          233 t
ORIGIN      
        1 ggcggacagg cccaccaccc gcacgctgcc cggcaggttg aggatcacct cctcccacac
       61 tgccccgcgg aatcgatcgg cgaggtagtg cacctcgtcc atcaccacgt aggccagccc
      121 accgagcgtg gtcgacccgg cgtagagcat gtttcgcagc acctcggtcg tcatcacgac
      181 caccggtgcg ttcccgttga tcgaggtgtc cccggtgagc aggccgacgg cgtgcgggcc
      241 gtgccgggtt accaggtcgg catacttctg gttggacagt gccttgatcg gtgtcgtgta
      301 gaagcacttg cggcgctgcg acagcgccag gtgtacggcg aactctccga tcacggtctt
      361 gcccgcaccg gtgggcgcgc acaccagcac cccatgcccg tcctcgaggg cctcgcacgc
      421 ctcccgctgg aaggggtcca gctgaaagct caagccgctg gcgaactcct tgaggcgcga
      481 cgcttcggtg cgggagcggc tggccactgc cccaggctag gccacgacgg tgagcgcagc
      541 gggttggcag tgcatggtca ctggcagggt gccctgtggc tcgccgtcgg cgtagaccgg
      601 ccacctcggg tcgccgtcga tgcgcaccgt cgcggcccgc agcacggtca cctcgggctc
      661 gtcaacatgg gcgccggtgc gcagcgacgg gaaaacccgc agcagccggc gcctgttcac
      721 cgcggtgatg gcgatgacgt cgagcaggcc gtcgtccagg gtggcatccg ggcagatccg
      781 cagccccccg ccgtagctgg gtccgttgcc gaccgcgacg agaatcgcct ccaggtccat
      841 cttgctgtcc ccggcgtgca cctggaccgg ccgcggagtc agtgccgcga gctcggcgag
      901 caccgccagg tcgtagcggc gtggcccggc cggccagcgc atcgcgttgg cccgggcgtt
      961 caccgcggca tcgaagccgg tgcacagcac cgtggtgtag caggctccgc cctccacacg
     1021 accgaggtca atgcgccgcc gctgcccact gcgcagcgca tcggcgacat gccgggcggc
     1081 gtgcaccgga ccggtaggga tgcccagcgc ggtggccaga tcgttgccgg tcccgctggg
     1141 tagcaccgcc agcgcggtct gtgtgccggc gacggcctga gcggccaggt gcacggtgcc
     1201 gtccccgccc agcacggcca gcacgtcgtc accgcgctgt gcggcctgcg cggccagcct
     1261 gctggtcgct gctgcgtcgg cggtcacggt ggcggtcagc tcgggcaccg cggcgcgcaa
     1321 ggtggccgcc acggtgccgg ccacacggac cgtcgcaccg cgaccggaat gggggttgac
     1381 gatcagcgtg acgggcacgg cgacgggcta tgtcgcgtcg tcgtagcgcg gggacgttgt
     1441 gccttccgat gccaccggct ccacccgatg gtccagcggg gaaggctcgt cgggatcgag
     1501 gtcgtcccag ccctgttcgg cgcgccgccg cgccagggtg cggtcgtgcg cccgagcaat
     1561 cagcaccgat gcctcgaaca gcaccaccag cgcaccggcc agcaccagca tggaaatcgg
     1621 gt
//
LOCUS       DATADN010000313         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_637977, whole genome shotgun sequence.
ACCESSION   DATADN010000313 DATADN010000000
VERSION     DATADN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637977"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VHH53_04130"
     CDS             <1..252
                     /locus_tag="VHH53_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEX2289384.1"
                     /translation="AGGTPVDMAYFTADRRPPADVCREAVCSADVFVGIVGFQYGSPV
                     RDLPELSYTQLEFQEASTAGMPTRHAQQAEPDGDVLPSL"
     gene            433..1604
                     /locus_tag="VHH53_04135"
                     /pseudo
     CDS             433..1604
                     /locus_tag="VHH53_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          335 a          618 c          589 g          309 t
ORIGIN      
        1 gccgggggta cgccggttga catggcgtac ttcaccgccg accggcggcc gccagcggac
       61 gtctgtcggg aggcggtgtg cagcgctgat gtgttcgtgg ggattgtggg tttccagtac
      121 ggctcgccgg tgcgcgatct cccggagctg tcctacacgc agttggaatt ccaggaggcc
      181 agtacggcgg gcatgccgac acgccacgcg cagcaagcgg agccggacgg tgacgtccta
      241 ccgtccctgt aaaacgtagg tggcctgctc acagatcatg tcgggtgtct ttggaccgac
      301 gagaggagca ggccgatggg aagggtagcg ccgtcagtga tcgctcggga gcagctgcat
      361 gggttgctgg ccggtggcgt cgagcgtgag tcgaacatca tctcggcact ggttgaggcg
      421 atggtccggc tcatggtgca ggaggtcctt gaaggcgagc agggcgattt ccttggcggc
      481 cggggccgtt atgagcgtcg cgcggagggc caagtcggct cccgcaatgg ctatgaaccg
      541 ggccgggtgc gcaccgcgga gggcgcggtc ggtgtcgcgg tcccgcaggt ccgcggtgct
      601 ggtgagccgt tccgatcaac cctgatgggc tttctcgatg gcaactctga tgtgctggag
      661 cggctggtgt gcgagatgta tgcgcgaggg ctgtccaccc gggatgtgga agacgccttt
      721 cgcgatgcca ccggtgaatt gctcatttcc aaaaccgccg tcagcgggat caccgaccga
      781 ctctgggagg actaccaggc cttcatcacc cgcgacttgt ccgacatcag cgtggagtat
      841 ctgttcgtcg acgcgatctt tgaatcgctg cgccgccacg gcgccaagga ggccctgttg
      901 gtcgcctggt gtattgcctc cgacgggcgc aaacacctgc tgcacctggc cgtgggcaac
      961 aaggaaagcc aaccgtgctg gactgccttc tttcgaacat gctcaaccgg ggcatgcgac
     1021 tgccgaccac catcacctcc gacggagcac cagggctgat caaggccatc acggtgtgct
     1081 tcccggccag catccggatc cggtgctggt ttcaccggct gggcaacctc cgcgccaaac
     1141 tgcccgacga aaccgccggc gaagtcctgg cccacgtcta cgccgtccgc gacgcaccca
     1201 ccctagacgc cgcccgcgtc gccgcggacc ggtttgccaa cacctacagc cgggagtacc
     1261 cagcggcagt ggcctgcttc accgacgacc tagaggcgct gctggccatc caccgggtgc
     1321 cggtgcgcca ccgcatccgc gtgcgcacca ccaacctggc cgaacgcagc ttcgccgaag
     1381 aacgccgacg caccaaggtc atcccccgac tcatggacga gcaggccgcg atgaaactgg
     1441 tgtacgcgac gatgatccgt gccgccgacc gctggtgccg cgtgtcaatc accgacctgg
     1501 aacgccacca gctcaccctg ctgcgaaccg aactcgggct cgacccaccc ccaagcaaca
     1561 ccgacgatcg caagaccaac cgacaaaaga acctagccgc atgatcatca ggccaccgat
     1621 ttacagggcg ctcaggactt gaccgagccg gaccgagctg gcagaagaag tcaagcagcg
     1681 gttcgccggt gaattcccca ccgagtaact cggccaccac cgtcacttca gccaccgcat
     1741 ctcaagcggt cgcgctgcat tctcttgctt agggatctcg tcatggccga gctgcgtgcg
     1801 gggcaccccc cgacgcctgc cgccacgcca tcgccgctga cttcgaactc a
//
LOCUS       DATADN010000314         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1396848, whole genome shotgun sequence.
ACCESSION   DATADN010000314 DATADN010000000
VERSION     DATADN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396848"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VHH53_04140"
     CDS             <1..346
                     /locus_tag="VHH53_04140"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289385.1"
                     /translation="TDAQVRRPAIADLLAKTQLHMDPTIPANFDEMRTTVSVRLQDGK
                     EYSVRCDRPRGIWGNPLTREERLTKVRQCAARVLPEADIERLIEVVEDLEHASSQDVS
                     VLMGLLARAPAS"
     gene            544..1512
                     /locus_tag="VHH53_04145"
     CDS             544..1512
                     /locus_tag="VHH53_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX2289386.1"
                     /translation="MKAVRVHKPGGPEGMVYEDVPDPKAGAGQAVVKVEAIGLNFAEV
                     NQRRNANPASLPMPIGGEAAGTVVEVGAGVSQFKVGDRVAFNGVPGAYAEMVAAPAAR
                     LVRIPDNVTTKQAAAALLQGMTAHYLACSTYPLGANDTCLVHAAAGGVGLLLCQIASQ
                     RGARVIGTVSTEEKAKAAREAGASEAILYTQLDFAEEVKRLTGDRGVDVVYDSVGQTT
                     FAKGLDCLRPRGMMVVYGQSSGPVAPVDTGLLSTKGCLYLTRVNLGTYTATRQELEER
                     SGDVFHWVGSGQLKVHIFGEYPLAEAAKAQAALDHRETIGKVLLVP"
BASE COUNT          271 a          481 c          538 g          227 t
ORIGIN      
        1 tacagacgca caggtccggc gccccgcgat cgcggaccta ctggcgaaga cccagctgca
       61 catggatccg acgatccccg cgaacttcga tgagatgcgc acgaccgtct cggtccggct
      121 gcaggatggc aaggaatact cggtccgctg tgaccggccg cgtgggatct gggggaaccc
      181 cctgacgcgt gaagagcgac tgaccaaggt gcgccaatgc gccgcccggg tgctgcccga
      241 ggcggacatc gagcggctga ttgaggtggt ggaggacctg gagcatgcct ccagccagga
      301 cgtgtcggta ctgatggggc tgctggcccg ggcaccggca tcttagggcc tgcagccgtc
      361 ggggtctgaa cgcgtgtagg gggtagggcc gcagcgcgtg gcagataacg tcggttccag
      421 gtgggtgccg cctcctgggg atggcggcaa caacccgtag cacgtcacgt cggcagcagg
      481 aacctggccg atgccaaacg cgttacgggc atttcgcacc actgtatggg aaggagggtt
      541 gccatgaaag ccgtacgagt ccacaaaccg ggcgggccgg aaggtatggt ctacgaggac
      601 gtccctgatc cgaaagcggg ggcaggtcag gcggtcgtca aggtcgaggc catcggcctg
      661 aacttcgccg aagtgaacca gcggcggaac gccaaccccg cctcactgcc gatgcccatc
      721 ggcggtgagg ccgccggcac cgtcgtggag gttggggcag gcgtctcgca attcaaggtc
      781 ggggaccgcg tcgcctttaa cggcgtcccc ggcgcctacg ccgagatggt cgcggctccg
      841 gcggcgcgac tcgtccgcat ccccgataac gtgaccacga agcaagcggc ggccgccctg
      901 ctgcagggga tgacggcaca ctacctggcc tgtagcacgt accctctcgg ggcgaacgat
      961 acctgcttgg tgcacgcggc cgcgggcggc gtcggcctgt tgctgtgcca aatcgccagt
     1021 cagcgcggcg cccgggtcat tggcacggtg tcaacggagg agaaggctaa ggcagcgcgg
     1081 gaggcaggcg ccagcgaggc gatcctgtac acgcagctcg acttcgccga ggaggtgaag
     1141 cgcctcacgg gagaccgcgg cgtcgacgtg gtctacgaca gcgtcggcca gacgaccttc
     1201 gctaaaggcc tggactgcct ccgcccgcgc ggcatgatgg tggtctacgg gcaatccagc
     1261 ggccccgttg cgcctgtcga tacgggccta ctcagcacca agggctgcct ctacctcacc
     1321 agggtgaatc tcggcaccta cacggcgacg cgccaggaac tcgaggagcg ctcgggcgac
     1381 gtctttcact gggtcgggag cggtcagctg aaggtccata tcttcggcga gtacccgctg
     1441 gcggaggcgg ccaaagcgca ggccgcgctc gatcaccgtg agacgatcgg caaggtcctc
     1501 ttggtgccat gagccgc
//
LOCUS       DATADN010000315         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_947289, whole genome shotgun sequence.
ACCESSION   DATADN010000315 DATADN010000000
VERSION     DATADN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947289"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(604..1206)
                     /locus_tag="VHH53_04150"
     CDS             complement(604..1206)
                     /locus_tag="VHH53_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289387.1"
                     /translation="MTADTSANDLDCQITALTEIVGALAATVSQLNARVDRLTSANRP
                     GDRGDRGDGHDEEPENHDPAAWVWFTPPAAAEEDPDIDGDPRITVDNFVTWYNVTYVG
                     VEGSRAKPIPDCWRQHPGLAMEVANLAYSWRAANMGQPANVREAQYWHHQTRPGFTDR
                     LAREWAHTECLDGEHRAAGAHERPDQFTLAEQLHPVGDGV"
     gene            complement(1203..>2249)
                     /locus_tag="VHH53_04155"
     CDS             complement(1203..>2249)
                     /locus_tag="VHH53_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HEX2289388.1"
                     /translation="VGVGGNDKVFRDRAKTVLQAYLMASALHRYDVGALVRWAVSKPP
                     DQEPIKLLHDGGYPELARNLRAEIGMVAETSDAVWMSVRRVIEPLMDPRLRQLCTPHP
                     GEDFDARSFITNQGSLYLVAGQHQAAQAAPLLTALVEYWLTTAQEMALDYPHRRVDPP
                     VTTVLDELTNATPVPQLPDIVSDSAGRGVLIHWAAQSLAKLEDVYTPARARQLLDNTT
                     TLSVWGGLKDQRTLEWISLLAGHHERLRYQQHSNGMLTPGRTAIGTETVPTYRPGAIR
                     TVPRGRVLVIHRTLAPILARTVDVAKRPDWSSICADIQTVRAGAAPVSPTGYALAPSE
                     RTLAAATPEAVPRW"
BASE COUNT          303 a          715 c          824 g          407 t
ORIGIN      
        1 cggcgggccg gccaacgtcc gctcttcggc agaggaggac gcggacgcgg gctacccttc
       61 cacgactact ccagcacgcg cgtccaccga gcctggccga aaggtggagc gcggtcatct
      121 tcgcagcttt acgactcgca gtgatttggt cgccacatcc ggatttcgtc gtcgaagtcg
      181 ccatcgcgga cgtctctgcg gcgcggtcac gtagccgatc tcgatcgcct ggacgaacat
      241 ctgcagcaca tgcatcggcg agtcctatag ccgaaccccg accataggtt ggccacccgc
      301 ctcagttgcc gccccacgtc gccaggtcgg tggcgatgcc ctcgtgcagc tgcgcgatgg
      361 cctaaaccca gtcgttgatc ttcgtccgga atgggcacaa ggcccagcat ccgccgagct
      421 gacccgctca gacgggggct gccgtgactg cggcagcccg tagtgtccag ctactacctg
      481 gccgggcacg gtgccgccga tatggatgtt gacccagcga gcgtgagcag gtcctcgccc
      541 atgtccttac cgatctgacc atcgccgcac tccacagtct ccggcagtgc gagcacagtc
      601 gtgtcagact ccgtctccca cggggtgaag ttgttcagca agagtgaatt ggtcggggcg
      661 ctcgtgcgcg ccggcggcgc ggtgttcgcc gtcgaggcat tcggtgtgtg cccactcgcg
      721 tgccaggcgg tcggtgaagc cgggtcgcgt ctggtggtgc cagtactgcg cctcacgcac
      781 gttggctggc tgccccatgt tggccgcccg ccagctgtag gcgaggttgg cgacctccat
      841 cgctaggccg gggtgctggc gccagcagtc cgggatgggc ttcgcgcggc tgccttcaac
      901 tccgacgtag gtcacgttgt accaggtcac gaagttgtcc acggtgatcc gcgggtcgcc
      961 atcgatgtcc gggtcctcct cggccgcggc tggcggggta aaccacaccc aggcggccgg
     1021 atcgtggttt tccggctcct catcgtggcc gtcgcccctg tcgccccggt cacccggtcg
     1081 gttggcgctg gtaagcctgt cgacgcgggc gttgagctgg gagaccgtgg cggccagcgc
     1141 cccgacgatc tcggtcagcg cggtgatctg gcagtccagg tcgttggcgc tggtgtcggc
     1201 ggtcaccagc ggggcaccgc ctcgggcgta gccgcggcga gggtccgctc ggagggtgcg
     1261 agcgcatagc cggtggggct gaccggtgcg gctccggctc ggacggtctg gatgtcggca
     1321 cagatgctgc tccagtcggg ccgtttggcg acgtcgacgg tgcgggcgag gatgggcgcc
     1381 agggtgcggt ggatgaccaa cacccgcccg cgtggcacgg tgcggatggc gccgggtcgg
     1441 taggtcggca cggtttcggt gccgatcgcg gtgcggccgg gcgtgagcat cccgttggag
     1501 tgttgctggt agcgcagccg ttcgtggtga ccggcgagta gggagatcca ttccagggtg
     1561 cgctggtcct tcaggccgcc ccacacgctg agcgtggtgg tgttgtccag cagttggcgg
     1621 gcgcgcgctg gggtgtagac gtcttcgagt ttggccagtg attgtgcggc ccagtggatg
     1681 agcacgccgc ggccggcgga gtcggacacg atgtcgggca gctgcggcac cggggtggcg
     1741 ttggtcagtt cgtcgagcac ggtggtgacc ggcgggtcga cgcggcggtg cgggtagtcc
     1801 agtgccatct cctgggcggt ggtcagccag tactcgacca gtgcggtgag tagtggggcg
     1861 gcttgcgcgg cctggtgctg gccggccacc agatacagcg agccctggtt ggtgatgaat
     1921 gagcgggcgt cgaagtcctc gcccggatgc ggtgtgcaca gctgtcgtag ccgcgggtcc
     1981 atcaagggct cgatgacccg tcgcacgctc atccacactg cgtcggaggt ctcggcgacc
     2041 atcccgatct cggcgcgcag gttgcgggcc agctccgggt agccgccgtc gtggagcagc
     2101 ttgatcggct cttggtcggg tggtttggac accgcccaac gcaccagggc gcccacgtcg
     2161 tagcggtgca gcgcggaggc catcaggtag gcctgcagga cggtcttggc gcggtcgcgg
     2221 aacaccttgt cgttgccgcc cacgcccac
//
LOCUS       DATADN010000316         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_638653, whole genome shotgun sequence.
ACCESSION   DATADN010000316 DATADN010000000
VERSION     DATADN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638653"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VHH53_04160"
     CDS             <1..149
                     /locus_tag="VHH53_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2289389.1"
                     /translation="RGLTPLTPEDVADVIAFAATRPSNVHLDQIVLKPRAQASATRAH
                     RDIR"
     gene            182..1546
                     /locus_tag="VHH53_04165"
     CDS             182..1546
                     /locus_tag="VHH53_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2289390.1"
                     /translation="MAALITALPAPRCATEVAMSDTPSTTPARSWVPRAHPLSWATLA
                     VTTGAILMTAVDGGILPAVLPAIQEDFELSNEQAGLVNSVFFAGLIVGALLFGWLSDR
                     IGTGYRRSWTWNVAMLIGILGGALTFGLAGSFAAFLLLRIPMGVSRGGSEPVNVALVS
                     EWWPKEHRGFAVGVHHTGFPLGQFLTGALIAAVLGVAGWQEAFLLIPLLGLPIIALQA
                     FIGTRRNQQKVYDWIDAHGYTRPLPELSTRPKGLGLAPVKEALANANVRWSVVLIFLF
                     LWAEAGAVTFLTVQLVNEGMDLGLAALLSGASGLTGWIGQVVWGAWSDHAGRKFAIRF
                     LVLGWALSLLLMIFITSPASAWIVLLVWGLFRNAPFPVVYALLIDSVPRAAGTAMGIM
                     IGVALGVSGVLAAWVSGVIIDAAGFTVHYVVLAAICLLGLLPLSRMTETVGTTTTETS
                     EVQK"
BASE COUNT          225 a          606 c          588 g          276 t
ORIGIN      
        1 accgggggct gaccccgctc acccccgagg acgtcgccga cgtgatcgcg ttcgcggcca
       61 cccggccgtc gaacgtgcac ctcgaccaga tcgtgctgaa gccgcgggca caggcgagcg
      121 ccacccgcgc ccaccgagac atcagatgag tggttgacgg cgggacgatc accggcggag
      181 gatggccgcg ctgatcacag cgctccccgc tccccgatgc gccaccgagg tcgccatgtc
      241 cgacacgccg tcgaccacgc ccgcccggtc gtgggtgccc cgcgcacacc ccctgtcctg
      301 ggcaaccctc gccgtcacca ccggcgccat cctgatgacg gcggtggacg gcggcatcct
      361 gcccgccgtc ctgccggcga tccaggagga cttcgagctc agcaacgagc aggccgggct
      421 cgtcaactcg gtcttcttcg ccggcctgat cgtcggagct ctgctcttcg gctggctgtc
      481 cgaccgcatc ggcaccgggt accggcgcag ctggacctgg aacgtcgcga tgctgatcgg
      541 gatcctcggc ggcgcgctga cgttcggcct cgccggcagc ttcgcggcgt tcctgctgct
      601 gcgcatcccg atgggggtca gccgcggcgg ctccgagccg gtgaacgtcg cgctggtgag
      661 cgagtggtgg ccgaaggagc accgcggctt cgccgtgggc gtgcaccaca ccggcttccc
      721 gctcggccag ttcctcaccg gcgcgctgat cgcggccgtg ctcggggtcg cgggctggca
      781 ggaggcgttc ctgctgatcc cgctgctcgg gctcccgatc atcgcgctgc aggcgttcat
      841 cggcacccgg cgcaaccagc agaaggtgta cgactggatc gacgcccacg ggtacacgcg
      901 gccactgccc gagctctcca cccgcccgaa gggtctcggc ctagcccccg tgaaggaggc
      961 actggccaac gcgaacgtcc gctggtcggt ggtgctgatc ttcctgttcc tctgggcgga
     1021 ggcaggcgcg gtcacgttcc tgacggtgca actcgtgaac gagggcatgg atctcggcct
     1081 ggccgccctg ctgtccggcg cgtccgggct caccggctgg atcgggcagg tcgtgtgggg
     1141 ggcgtggtcc gaccacgcgg gccggaagtt cgcgatccgg ttcctggtgc tcggctgggc
     1201 gctcagcctg ctgttgatga tcttcatcac cagcccggcg agcgcgtgga tcgtgcttct
     1261 cgtgtggggc ctgttccgca acgccccgtt ccccgtggtg tacgccctgc tcatcgactc
     1321 ggtgccacgc gcggccggca ccgcgatggg gatcatgatc ggagtggcac tcggcgtgtc
     1381 cggcgtgctc gccgcctggg tgtccggcgt gatcatcgac gcggccggct tcaccgtgca
     1441 ctacgtcgtg ctcgccgcga tctgcctgct cgggctgttg ccgctgtcgc ggatgaccga
     1501 gaccgtggga accacgacga ccgagacctc ggaggtgcag aagtgaccga cctgctcgaa
     1561 tccggtgccg agctggagaa ggtggccacc ggctcgatct gggcggaagg cccggtctgg
     1621 gtcgcggccg accgcacggt gcgctacagc gacatcccca acaaccgcat cctgcagttc
     1681 tccgaagcga gcggg
//
LOCUS       DATADN010000317         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1097805, whole genome shotgun sequence.
ACCESSION   DATADN010000317 DATADN010000000
VERSION     DATADN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097805"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1711)
                     /locus_tag="VHH53_04170"
     CDS             complement(119..1711)
                     /locus_tag="VHH53_04170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX2289391.1"
                     /translation="MNGGSLLLALLLLPLVGSVVVALMRSAPVAAKATAMAFALAELV
                     LAALAWSAYQPGGQRLQLLLSTEWIPAFGARFALGVDGIALVMIALLAVLVPVVMGSS
                     WAEKLPEGRTAAGFYALLLASQSMMIGVFAATDVFLFYLFFEVMLVPMYFLIGRFGGP
                     RRQYAAVKFFLYSLLGGLVMLASVIGIFVVSSQRLGQGTFDWASLVGIAGTIPQSTQI
                     WLFLGFFVAFAIKAPLVPLHTWLPDAGAEAPVGAGVLLVGVMDKVAIYGFLRYCLPLF
                     PDASRTLAPLVLVLAVAGVVYAALLAVGQSDLKRFVAYTSVAHFGFIALGVFAFSTQG
                     LAGAVLYMVNHGIATGMLFIVIGMLIARGGSRLIADYGGVAALAPVLAGMMLIAGLAS
                     LALPGTNSFVSEFLVLIGSFPRQPVYTIIATAGIILAALYVLWVYQRVMQGPVRGAAV
                     LGALGGGPGTAMAPEVAAQRQRERFPDLSLREVAVLAPLVALILLLGFYPQPVLDVIN
                     PSAGATLTEVGLTDPVVEQGGN"
     gene            complement(1708..>2026)
                     /locus_tag="VHH53_04175"
     CDS             complement(1708..>2026)
                     /locus_tag="VHH53_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX2289392.1"
                     /translation="VVGRQPVPVQRPQRVSLPVAAARADLYANAINETLIARPGTWLT
                     RALVFVDNKGVDGVVNGTAALLGGTSGRLRRLQTGFVRSYALGMLGGSVVVIALLLAV
                     SFS"
BASE COUNT          418 a          736 c          652 g          220 t
ORIGIN      
        1 gatgcacgcc atcccgagca ccaccagcag cggggcgatc gccccgtaat ccacggcagg
       61 catcggcacc cgcgggacct gactcagaag tccccccagg aactcaccgt cgagcacctc
      121 agttgcctcc ctgctcgacc accgggtcgg tcagcccaac ctcggtgagc gtggccccgg
      181 ctgaagggtt gatcacatca agtaccggct gcgggtagaa tccgagcagc aggatgagcg
      241 ccaccagcgg cgccagcaca gcgacctcgc ggagggacag atcagggaac cgctcgcgct
      301 ggcgctgcgc cgcgacttcc ggtgccatcg cggtcccagg gccaccgcca agcgccccca
      361 gcacagccgc gccccgcacc gggccctgca tgacccgctg gtagacccac agcacataca
      421 aggcggccag gatgatcccg gcggtggcga tgatcgtgta caccggctgg cgggggaacg
      481 acccgatgag caccaggaac tccgacacga acgagttggt gccgggcaac gccagcgatg
      541 ccagcccggc gatcaacatc atcccggcga gcaccggcgc caacgccgcc acacctccgt
      601 agtcggcgat cagccgggag ccgcctcgag cgatcagcat gccgatcacg atgaacagca
      661 tcccggtggc gatgccgtgg ttgaccatgt acagcaccgc ccccgccaac ccctgagtgg
      721 agaaggcgaa gacacccagc gcgatgaagc cgaagtgcgc caccgacgtg taggccacga
      781 accgcttcag atcggactgg ccgacggcca gcagcgcggc gtagacgacc ccggcgacgg
      841 cgagcaccag caccagcgga gccagtgtgc gggacgcatc gggaaacagc ggcaggcagt
      901 agcgcaggaa accgtaaatg gcgaccttgt ccatcacacc gaccagaagc acaccggcgc
      961 ccaccggggc ctcggcaccg gcgtcgggta gccaggtgtg cagcggcacc agcggcgcct
     1021 tgatcgcgaa agcgacgaag aacccgagga acagccagat ctgggtgctc tgcgggatgg
     1081 tcccggcgat gccgaccagg gatgcccagt cgaaagtgcc ctggccaagc cgctgcgagc
     1141 tgaccacgaa gatgccgatc acgctggcca gcatgaccag cccgccgagc agcgagtaca
     1201 ggaagaactt caccgccgcg tactgccgtc gcggcccacc gaagcggccg atgaggaagt
     1261 acatcggcac cagcatgacc tcgaagaaca ggtagaacag gaacacgtcg gtggcagcga
     1321 agaccccgat catcatcgac tggctggcca gcagcagagc gtagaaaccc gccgcggtgc
     1381 ggccctcggg cagcttctcc gcccaggagg accccatcac gaccggcacc agcacagcga
     1441 gcagcgcgat catcaccagc gcgatgccgt cgacgccgag cgcgaagcgg gccccgaagg
     1501 ccggaatcca ctcggtggac agcagcagct gcagccgctg tccccccggt tggtaggccg
     1561 accaggccag cgcggccagc accaactccg ccagcgcgaa cgccatcgcg gtggccttgg
     1621 ccgcgaccgg cgcggagcgc atcagggcga ccaccacgct gcccaccagg ggcagcaaca
     1681 gtaatgccag taacaagctg ccgccgttca cgagaagctc accgccagca gcaacgcgat
     1741 caccacgacc gagcctccca gcatgcccag cgcgtagctg cgcacgaagc cggtctgcag
     1801 cctgcgcagc cggcctgacg tgccgcccag cagcgccgcg gtgccgttga ccaccccgtc
     1861 gacgcctttg ttgtcgacga ataccagcgc ccgggtaagc cacgtaccgg gccgtgcgat
     1921 gagtgtctcg ttgatcgcgt tggcgtagag gtcggcccgg gcggcggcca ccggcagcga
     1981 gacccgctgc ggccgttgca ccggcaccgg ctgccggccg acgacc
//
LOCUS       DATADN010000318         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_638830, whole genome shotgun sequence.
ACCESSION   DATADN010000318 DATADN010000000
VERSION     DATADN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638830"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..1398
                     /gene="amrS"
                     /locus_tag="VHH53_04180"
     CDS             334..1398
                     /gene="amrS"
                     /locus_tag="VHH53_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX2289393.1"
                     /translation="MPTRYWHRLDDGRLQCDVCPRACRLHEGQRGLCFVRGRIDEQVV
                     MTSYGRSSGFCVDPIEKKPLNHFLPGSAVLSFGTAGCNLACRFCQNWDISKSREIDRL
                     TAAATPDVIAETAQRLGCRSVAMTYNDPVIFLEYAIDVADACRARGIKAIAVTAGYLT
                     DKPRRELFAHIDAANVDLKAFTESFYHRISAGHLQPVLDTLVYLRHTTDVWVEITTLL
                     IPGHNDSDTEIDAECAWIARHLGTEVPLHFTAFHPDFKMRGVPPTPPTTLTRARRIAQ
                     EHGLRFVYTGNVHDPDGGRTTCPSCGAAVIDRDWYAIHRYELTDDGRCTRCRSTIPGR
                     FDGPAGRWGTRRLPVSLAAR"
BASE COUNT          321 a          585 c          503 g          303 t
ORIGIN      
        1 aaccataagt ccgccgctgg ctggcctggc atcgctggac cctgcttgcc atgctcgctc
       61 acgccttcct cgccgtcatc gaacgcgcca aaaccagccg ctaccggcgc caagaagtga
      121 tcctcacatg atcacgattt acggctggag tatccagggt ggcagttggt ggcccgacgt
      181 gtgaggctgg atctggtggg cgtggtggac ttgcaggctc gcaagtcaac aagccaacaa
      241 acagcctgca acagccgggc cgtggcggcc agcctcgcct agagtcagga gaacgcagtg
      301 accgtcgaga tcagtttaag tgacccgtgc acggtgccaa ccaggtactg gcatcggctg
      361 gatgatggtc ggctccaatg tgatgtgtgt ccgcgggcat gtcggttaca tgagggccag
      421 cgggggctgt gttttgtgcg tggccgcatc gacgagcagg tggtgatgac ctcctacgga
      481 cgatccagcg ggttttgcgt cgaccccatc gaaaaaaagc cgctcaacca ttttttgccc
      541 ggcagcgccg tgctctcctt tggtactgcg ggctgcaatc tggcttgtcg gttctgccaa
      601 aactgggaca tttccaagtc ccgggagatc gatcgactga ccgcagcagc gacgcccgat
      661 gttatcgccg agactgcgca acggctcggc tgccgcagcg tcgcgatgac gtacaacgat
      721 ccggtgattt ttctcgagta cgccatcgac gtcgccgacg cctgccgtgc ccgcggaatc
      781 aaagccatcg cggtcaccgc cggctacctc accgacaaac cccgtcggga gctgttcgcc
      841 cacatcgacg ccgccaacgt ggatctcaag gcgtttaccg agtcttttta ccaccgcatt
      901 tccgccggtc acctccagcc ggtcctcgac accctggtgt acctgcggca caccaccgac
      961 gtctgggtgg aaatcaccac gctgctaatc ccgggacaca atgactcaga caccgagatc
     1021 gacgcggagt gtgcctggat cgcccgccac ctaggtactg aggtgccgtt gcacttcacc
     1081 gccttccacc ccgacttcaa gatgcgtggc gtgccgccga ctccaccaac cacactcacc
     1141 cgggcccgca gaatcgcgca ggagcatggg ctgcgctttg tctacaccgg caacgtccac
     1201 gacccagacg gcgggcgcac tacctgcccc agctgcggcg cagctgtcat cgaccgggac
     1261 tggtacgcca tccaccgcta cgagctcacc gacgacggac ggtgcacacg ctgccgcagc
     1321 accattcccg gccggttcga cgggcccgcc ggtcgctggg gcactcgccg gctgccggtg
     1381 tcactcgctg cacgctgagg cgaactgaat ctctgcccgg ctgccgtcaa caaccatcca
     1441 cacagcacat ccggtggcac gcagggccgc cgtacgccgg gcatcatagg cgccaagcag
     1501 acgatcaact acgtgcggcg tgctattggc gtgctattaa ccccggtgag cacggtcaat
     1561 agcggggccc tgtgacaccg cccttccgca gaccgaggcc cccaacccac gcgtggagcg
     1621 cagccttcca aactggggct cacaggtgat catcgccacc cgtcgttgga gtcctcgggg
     1681 acggccccta actgggggac cgtattccag tg
//
LOCUS       DATADN010000319         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_947527, whole genome shotgun sequence.
ACCESSION   DATADN010000319 DATADN010000000
VERSION     DATADN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947527"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VHH53_04185"
     CDS             complement(<1..346)
                     /locus_tag="VHH53_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX2289394.1"
                     /translation="MSTTYGGAGLGMLAAELTTVLGPAAVITDRQRLRTYECDGLAQY
                     RVVPGIVVLPESAEQIAAVVKACAAAGVPFVARGSGTGLSGGALPHAEGVLIVTSRMR
                     RILEVDPANERAI"
     gene            complement(495..2126)
                     /locus_tag="VHH53_04190"
     CDS             complement(495..2126)
                     /locus_tag="VHH53_04190"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX2289395.1"
                     /translation="MAQLVLGPMLRYVDSTSAVVWVETDTPCRVEVLGVSCPTFALHG
                     HHYGLIELSDLAPGSSLPYTVSLDGEQAWPEPGSELPPSRIRTIDPDAPVRLVFGSCR
                     TSVPHDMKHNLTHGFDMLRCFAYELRNTDEPAWPTALIILGDQVYADEPPETMLEFIR
                     QRRGTDDPPHDELRDFDEFAELYRIAWTDPDVRWLLSTVPTSMIMDDHDLRDDWNTSR
                     AWREQMAQVPWWRQRVIGALGSYWIYQHVGNLSASERAGDTLLSALRNTDGDGGVLLD
                     DFAERSYDQPCGAAFSYVRDFGQTRFVMLDSRCGRLLTPGKRAIMDDDGWKWFAEQVT
                     GQYEHLVIGTSLPYLLPAGLHYLEAWNEAVCDGAWGDRAAWVAEKIRQGIDLEHWAAF
                     RRSFEAVAHLLIDLVAGRHRPPPSSVGFLSGDVHYAYLARALLPEAANGSATTVYQVV
                     CSPIRNPLSGPVRLLNALASFAVAGLVGRGLAKAARVPPSPFDWTVTSGPYFRNVLST
                     LEIDGERVAVRWIAPCASTDDPPPLQEIGTQRIHR"
     gene            2214..>2718
                     /locus_tag="VHH53_04195"
     CDS             2214..>2718
                     /locus_tag="VHH53_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2289396.1"
                     /translation="MEHDLLIISNAKAGSADQPVLQEVLNVLRRGGTDVHSETLGSPD
                     ELDALLGAHPHRIPVAAGGDGTLHLLVAALHARGELASRTIGLVPLGTGNDFARTVGI
                     PLTAATAARVVLQGSPVEMDLLTDDAGGVVINAVHLGIGAQGAREGEPLKPVLGMLAY
                     RIGALQAG"
BASE COUNT          454 a          920 c          913 g          431 t
ORIGIN      
        1 cgatcgcgcg ctcgttggcc gggtcaacct ccaggatgcg acgcatccgc gaggtgacga
       61 tcagcacacc ctcggcgtgc ggcagcgccc cgccggatag cccagtaccc gagccgcgag
      121 ctacaaacgg tactcccgcg gcggcgcacg ccttcaccac cgcggcgatc tgctctgcgg
      181 actccggcag caccacgatg cccggcacca cgcggtactg ggccagcccg tcgcactcgt
      241 acgtgcgcag ccgttgccgg tcggtgatca ctgccgcagg cccgagcaca gtggtgagct
      301 ctgcggccaa catccccaac ccagcgccgc cgtatgtcgt actcatcgcc cgctcccctg
      361 gtccggcatc gccttcgccg gcccttcgcc cagtgtcgca gcttgccaag gatgcggttg
      421 gcccgaaacc gccaaagcca ccccaaagta caaggtagtc gtcagatcgg tcgcacagct
      481 cggcgaccac caggtcaccg atggatgcgc tgggtaccga tctcctgcag cggaggggga
      541 tcgtcagtgc tggcacatgg cgcgatccag cgcacagcta cccgttcgcc gtcgatctcc
      601 agcgtgctga gcacgttgcg gaagtagggg ccgctggtca ccgtccagtc gaacggtgac
      661 gggggcacgc gggcggcctt ggcgagacca cggcccacga gcccggcgac cgcgaagctc
      721 gccaacgcgt tgagcaggcg gactggcccg gacagcgggt tgcggatcgg tgagcagacg
      781 acctggtaga cggtcgtcgc tgagccgttg gcagcctcag ggagcaaggc gcgggctaga
      841 taggcgtaat gcacgtcacc ggataggaag ccgacgctgc ttggtggcgg ccggtggcgc
      901 cccgccacca gatcgatcag caggtgtgcg accgcctcga acgagcggcg aaacgccgcc
      961 cagtgttcga ggtcgatgcc ctgtcgaatc ttctctgcga cccaggccgc ccggtcgccc
     1021 cacgcgccat cgcagacagc ctcgttccag gcttccagat aatgcaggcc agccggcagg
     1081 aggtaaggca acgacgtgcc aatcaccagg tgctcgtact gaccggtgac ctgctcggcg
     1141 aaccacttcc acccatcgtc gtccatgatc gcccgcttac ccggggtcag cagccgcccg
     1201 caccggctgt cgagcatgac gaaccgggtc tggccgaagt cgcggacgta gctgaacgcc
     1261 gcgccgcacg gctggtcgta ggaccgctcg gcgaagtcgt cgagcagcac gccgccatcc
     1321 ccgtcggtgt tgcgtagcgc gctcaggagc gtgtcgccgg ctcgctcgct cgcggacaga
     1381 ttcccgacat gctggtagat ccagtacgag cccagtgcac cgatcacacg ctgtcgccac
     1441 cacggcacct gggccatctg ctctcgccac gcccgcgagg tattccagtc gtcccgcagg
     1501 tcatgatcgt ccatgatcat ggaggtcggc acggtggaca gcagccagcg gacgtccgga
     1561 tcggtccagg cgatccggta gagctcggcg aactcgtcga agtcccgcag ttcgtcgtgg
     1621 ggcgggtcgt cggtgccgcg ccgctgccgg atgaactcca gcatggtctc gggtggctcg
     1681 tcggcataga cctgatcacc gagaatgatc agcgcggtag gccacgccgg ctcgtcggtg
     1741 ttccgtagct cataggcgaa gcaccgcagc atgtcgaacc cgtgtgtgag gttgtgcttc
     1801 atgtcgtgcg gaacgctggt tcggcaggag ccgaacacca gccggaccgg cgcgtccgga
     1861 tcgatcgtgc ggatgcggct gggcggcagc tcgctgccgg gctctggcca cgcctgctcg
     1921 ccgtccaggc tgaccgtgta gggcagcgaa ctgcccggtg cgaggtcgct gagctcgatg
     1981 agcccgtagt ggtgcccgtg cagcgcgaac gtcgggcagg agacgccaag aacctcaacc
     2041 cggcacggcg tgtcagtctc gacccatacc acggcgctgg tcgagtcgac gtagcgcagc
     2101 atcggaccaa gtacgagctg cgccattgtt acctccaccg ttctacctgg gaattgtacc
     2161 ctaataagcg gtatgtgtca gcggtatcgg caatcgctgg ttgtctgagc ggtatggagc
     2221 atgacctgct gatcatctcg aacgccaagg ccggcagcgc ggaccagccg gtactgcagg
     2281 aggtgctcaa cgtgctacgc cgtggcggca ccgacgtgca cagcgaaacg ctcggcagtc
     2341 cggacgagct ggacgctctg ctcggtgcgc atccgcaccg gatcccggtt gcagccggcg
     2401 gcgacggcac gctgcacctg ctcgttgccg cgctgcatgc tcgtggtgag ctggcgagcc
     2461 ggacgatcgg tctggtgccg ctgggtaccg ggaatgactt cgcgcgcacg gtgggcatcc
     2521 cgctgaccgc tgccacagcc gcccgcgtcg tgctgcaggg ctcgcccgtc gaaatggacc
     2581 tgctgaccga cgacgcgggt ggggtggtca tcaatgcggt gcacttgggc atcggcgctc
     2641 aaggtgcccg cgagggggag ccactgaagc cagtgctcgg gatgctcgcc taccggatcg
     2701 gtgcgctgca ggccgggc
//
LOCUS       DATADN010000320         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_947744, whole genome shotgun sequence.
ACCESSION   DATADN010000320 DATADN010000000
VERSION     DATADN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947744"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="dnaJ"
                     /locus_tag="VHH53_04200"
     CDS             complement(<1..1011)
                     /gene="dnaJ"
                     /locus_tag="VHH53_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX2289397.1"
                     /translation="MAKDYYAILGVPRNANVEQIKRAYRKLARELHPDVNPDAQAQQR
                     FQDVTAAYEVLTDPQKRQIVDLGGDPLEPGRGGGDPFGGFGFGDIMDAFFGAAGGPGR
                     RGPRSRVQSGSDALIRLQLTLEECASGASRELTVDTAVLCGECSGSGCAPGTGPQTCD
                     TCSGSGEVQNVQRSFLGQVVTSRPCPVCRGIVEVIPDPCRQCGGDGRIRARRNVTAKV
                     PAGVADGMRVRLAGQGEIGPGGGAAGDLYVEVQEVPHETFTRDGADLHCTVRVPMTSA
                     ALGTTVALDTLDGTEEVRVEAGTQCATVLTLRGRGMPRLRSTGRAEGRGDLFVHLDVV
                     TPT"
     gene            complement(1077..>1707)
                     /locus_tag="VHH53_04205"
     CDS             complement(1077..>1707)
                     /locus_tag="VHH53_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEX2289398.1"
                     /translation="STVRHLEVLAVTPARLMLVLITDAGRVDQRLLDLGDVISEDQVA
                     QLRTLLNQALSGRRLADASVAVADLPEQTPVELRDAMTRASTVLIETLVEHPEERLLL
                     GGTANLTRNATDFPGSLRQVLEALEEQVVVLKLLAAACDPGTITVRIGEENEAEEMRS
                     TSLISIGYASHGTVLGGMGVVGPTRMDYPGTMAAVRAVARYVGEILTTR"
BASE COUNT          270 a          630 c          538 g          269 t
ORIGIN      
        1 cgtcggggtc acgacgtcga ggtggacgaa tagatcaccg cgtccctcgg cacggcccgt
       61 actgcgcagc cggggcatcc ctcgtccccg cagcgtgagc accgtcgcgc actgggtgcc
      121 cgcttcgacc cggacctcct cagtgccgtc gagggtgtcc aacgcgacgg tggtgcccag
      181 cgcggcggag gtcatcggca cccgcaccgt gcagtgcagg tcagcgccat cccgggtgaa
      241 cgtctcgtgc ggtacctcct gcacttcgac gtagagatcg ccggccgcac caccaccggg
      301 gccgatctcg ccctgcccgg ccagccgcac ccgcatcccg tcggccactc cggccggcac
      361 cttggcggtg acgttgcggc gggcgcggat ccgtccgtcc ccgccacact ggcggcaggg
      421 atccgggatg acctcgacga tgccgcggca caccgggcac ggccgcgagg tgaccacctg
      481 accgaggaag gagcgctgca cgttctgcac ctcgcccgaa ccgctacagg tgtcacaggt
      541 ctgcggcccg gtacctggcg cgcacccgga accgctgcat tccccgcaca gcactgcggt
      601 atccacggtg agttcgcgcg acgcaccgct ggcgcactct tcaagggtga gctgcagccg
      661 gatcaacgcg tcagaaccgc tctgtacccg gctacgcggc cctcgccgcc cgggtccgcc
      721 ggctgcaccg aagaaggcgt ccatgatgtc gccgaagcca aacccgccga aggggtcacc
      781 gccaccgcgc ccgggctcca gcgggtcccc gccgaggtcg acgatctggc gcttctgcgg
      841 gtcggtcagc acctcgtagg cggcggtgac gtcctggaaa cgctgctgcg cttgcgcatc
      901 gggattgaca tcgggatgca actcacgagc cagcttgcgg tacgcgcgtt tgatctgctc
      961 cacgttggca ttcctcggca cgcccaggat tgcgtagtag tccttggcca cgctggtgtc
     1021 ctcctgttgc agccctcgcc gcggtgctgt cgtgcgatat ccgtgagcgc cggtgctcaa
     1081 cgggtggtca ggatctcccc cacgtatcgg gccaccgcac gcaccgcagc catggtgccc
     1141 gggtagtcca ttctggtcgg tccgaccacg cccatacctc ccagtaccgt gccgtgggag
     1201 gcgtagccga tcgagatcaa cgacgtactg cgcatctcct cggcctcgtt ctcctcaccg
     1261 atccgcaccg tgatggtgcc cggatcgcag gctgcggcca gcagcttgag caccaccacc
     1321 tgctcctcca gcgcctcgag cacctgccgc aacgaacccg ggaagtcagt ggcgtttcga
     1381 gttagattag cggtaccgcc cagcagcagc cgctcctccg gatgttccac caaggtctca
     1441 atcagaaccg tcgaggcccg cgtcatcgcg tcccggagct cgaccggagt ctgctcgggt
     1501 aagtcagcca ccgccaccga ggcgtctgcc aaccgacggc ccgacagcgc ctggttgagc
     1561 aaggtccgta actgcgccac ctgatcctcg gagatgacgt cacccagatc gaggaggcgc
     1621 tggtccaccc gccccgcgtc ggtgatgagt acgagcatca accgggccgg cgttaccgca
     1681 agcacctcca ggtggcgcac cgtggag
//
LOCUS       DATADN010000321         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_344972, whole genome shotgun sequence.
ACCESSION   DATADN010000321 DATADN010000000
VERSION     DATADN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344972"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..340
                     /locus_tag="VHH53_04210"
     CDS             158..340
                     /locus_tag="VHH53_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HEX2289399.1"
                     /translation="MDLPAVTWRKSSRSESQSACVEVALTGTGAVRDSKNPNGPTLAV
                     DLVPMLAAIKSGRLDH"
     gene            complement(636..1763)
                     /locus_tag="VHH53_04215"
     CDS             complement(636..1763)
                     /locus_tag="VHH53_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2289400.1"
                     /translation="MRKPSTVIAAAMTALCVLPFSVPAATAATTPPRPMVGLSLPAPI
                     GPAAVGRTTLALTDRDRVDPWAPETGPRRLMVTLYYPALRRTGSPAPYMDIAEATALL
                     DFGQLGPELSPELLANTRTWSHAHARPLPGRYPLVLVSPGFTVARHSLTALAEDLASR
                     GYVVAAVDHAFESVAAKFPGGVLPCLACDLPQQIGIAPIALNRALDLSFVLSELRHHP
                     LVDSRRVAVVGHSMGGDAAATTMRADPRVDASVNLDGPLLPPDPLDRPFLLLGGLDEH
                     RPGGSFDPTWDVAWPHMTGWKRWLTVTDADHYSFTDLNLLVQQAGVNLPIPLDPARGI
                     HLTRTYVAAFADRHLRGITQPLLAGPTTDFPEITFHHQGRP"
BASE COUNT          329 a          615 c          769 g          301 t
ORIGIN      
        1 gcacgtcgag ctggcccggt ctggcgcgtt cctggacgag gaaccgttca cggctccgta
       61 cctgcgccgg atgtcgcggc tcaccgaggt cgcgctgagt gcgacagaat cgaaccgacg
      121 tatggtcgac gttcgagacc ggatggagcc ctgatctatg gatcttccag cagtaacgtg
      181 gcgcaagtcg tcgcggtcgg agtcgcagtc cgcctgtgtt gaggtcgcgc tcaccggcac
      241 cggcgccgta cgcgacagca agaaccccaa cgggccgacg ctcgccgtcg acctggtccc
      301 catgctggcg gcgatcaagt ccggacgcct ggaccactga ccgcggagac caccgcaaga
      361 gccccgggca gactctggcc tggggctcgt tgccatcctg gcaaggacaa acgcgccact
      421 cgcctaccgg cacggatggg tgcctacgca tccatgacca gaccgcggcc ggacttatca
      481 gttgggagcc ccgatcgggt ctcggcggtg ttctcggtgg cgcggtgctc gaccgaggcg
      541 gccggctcgg tcacggatgc ggccggttcg gtctcgccgc gtcgccgaga gcgggagcca
      601 ggcaatgtac acacagctgt gcgtgtgttc gtcgcctacg gccggccttg gtggtggaag
      661 gtgatctcgg ggaaatcggt tgtggggccg gcaagcagtg gttgggtgat gccgcgtagg
      721 tgtcggtcgg cgaacgcggc tacataggtg cgggtcaggt ggatgccgcg ggcgggatcg
      781 aggggtatcg gcaggttgac gccggcctgc tggacgagca ggttcaggtc ggtgaaggag
      841 tagtggtcgg cgtcggtgac ggtcagccag cgcttccagc cggtcatgtg tggccacgcg
      901 acgtcccagg tggggtcgaa gctgccgccg ggacggtgct cgtcgaggcc gccgagcagg
      961 aggaagggcc ggtccagcgg gtcgggcggc agcagcggtc cgtcgaggtt gacgctggcg
     1021 tcgacgcggg ggtcggcgcg catcgtggtg gccgcggcgt cgccgcccat ggagtgcccg
     1081 acgacggcga cccgccgtga gtcgaccagg gggtggtggc gcagctcgct gagcacgaag
     1141 gacaggtcca aggcccggtt gagcgcgatg ggggcgatgc cgatctgctg gggtaggtcg
     1201 caggccagac agggcagcac accgccggga aacttcgcgg cgaccgactc gaaggcatgg
     1261 tcgacggcgg cgacgacgta gccgcgcgaa gcgaggtcct cggcgagggc ggtgaggcta
     1321 tggcgggcaa cagtgaaacc gggtgagacc agcacgagcg ggtaccggcc gggcagcggc
     1381 cgggcgtgcg cgtgcgacca ggtgcgggtg tttgccagca gttcggggct gagctccgga
     1441 ccgagctggc cgaagtcgag cagcgcggtg gcctcggcaa tgtccatgta gggtgccggg
     1501 ctgccggtgc ggcgcagggc cgggtagtac agggtgacca tgagccgtcg cggcccggtt
     1561 tcgggcgccc agggatcgac acggtcgcgg tcggtcagcg ccagcgtggt acgtccgaca
     1621 gcggcaggcc caatcggcgc cggcagcgac agacccacca tgggccgagg cggtgtcgtg
     1681 gcagccgtgg cggcggggac agagaaggga agcacgcaca gcgcggtcat ggcggcggca
     1741 atcaccgtgg agggctttcg catgcctcga ctgtgccgaa gacccacgac cgaaacatca
     1801 ggagccaccc ggaccaccac ccggagacaa gcccttaaga tcagcgcacg gcaccatcgg
     1861 cagcggtgcg tgacgccggg cattcacccc ccgacgccgt cccctctgtg tcaagatcaa
     1921 gtgaggcaag gtcgagtttg agccggcgcc tatcttgagg agttttacac cagtggtttc
     1981 tcgcgtatac ctttccgatt cgcttgccga accc
//
LOCUS       DATADN010000322         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_493551, whole genome shotgun sequence.
ACCESSION   DATADN010000322 DATADN010000000
VERSION     DATADN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493551"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VHH53_04220"
     CDS             <1..747
                     /locus_tag="VHH53_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005445932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2289401.1"
                     /translation="SSEGAVLRSVLRGDCHTHSDWSDGGSPIEEMAETARALGHEYFV
                     LTDHSPRLTVANGLSAERLRHQLDVVAALNEELAPFEILTGIEVDILSDGALDQSEEL
                     LASLDLVVASVHSKLRMPAAEMTERMLTAVANPHVDVLGHCTGRMVTGSRKRPESQFD
                     ADAVFAACAEHGVAVEINCRPERLDPPKRLLRLAVEAGCLVAIDSDAHAPGQLDWLAY
                     GCERAVACGVPADRVVNTWPRGKLRNWTAR"
     gene            complement(832..2184)
                     /locus_tag="VHH53_04225"
     CDS             complement(832..2184)
                     /locus_tag="VHH53_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2289402.1"
                     /translation="MTGPVRVAVIGSGPSGLYAADELSKHGGVSVDVVDRLPCPYGLL
                     RYGVAPDHLKMKSLEITLRKILERPGVRFLGGIELGAGISVEELREYYDAVIYATGSS
                     VDRRLSIPGEDLPGSFSATQFVAWYCGHPDAEVDAFTLDARSAVVVGVGNVAVDVARI
                     LAKTTEELQSTDMPDHVLKVLDSSAISDIYIVGRRGPAQAKFTTKELRELGELANADV
                     VVDRAELVLDDAGEKILETNKTVRRNLETLQEWSTRSQQGRPRRIHLRFLLSPVAVHG
                     AGRVEAVECERNALDGCGGATGTGAKVTLEAQLVLRSVGYRGLPLPGVPFNERTGVIP
                     HVAGLVQRDGCAATGEYVVGWIKRGPTGVIGTNKGDAKETVEQLLADVEALPRARHRE
                     PDAIIAKLAQRGVGVVTWAGWEAIDAAEQALGVAAGRTRIKIADREALLIAAAKALKG
                     "
     gene            complement(2220..>2677)
                     /locus_tag="VHH53_04230"
     CDS             complement(2220..>2677)
                     /locus_tag="VHH53_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2289403.1"
                     /translation="ELRAEGVTTAVATTGTSDWVLPLLDILFGLDAFDVVVTGSDITD
                     LKPDPAVYLEALARLRLPAANAVAVEDSANGVHAAVAAGLTCVAVVNGYTRDQDLSGA
                     VAVLDDVARPDGVSVLHGDPHLRDGLSVAALRRLVTAPPRHGGGVTSGP"
BASE COUNT          452 a          946 c          840 g          439 t
ORIGIN      
        1 agctcggaag gagcggtcct gcgctcggtg ctacgcgggg actgccatac ccattccgac
       61 tggtccgacg gtggctcacc catcgaggaa atggcggaga cggcacgcgc gctcggccat
      121 gaatacttcg tgctgactga ccactcaccg cggctgaccg ttgccaacgg cctgtccgct
      181 gagcggctgc gccaccagct cgacgtggtg gccgctctca acgaggagct cgccccattc
      241 gagatcctca ctgggatcga ggtggacatc ctctccgacg gtgcacttga tcaatccgaa
      301 gagttactag catcgctaga ccttgtcgtc gcgtcggtgc attcgaaact gcggatgccg
      361 gccgcggaga tgaccgagcg gatgctcacc gcggtggcca acccgcatgt cgacgtgctt
      421 ggacactgca ctggccggat ggtcaccggc tcacgtaagc gccccgaatc gcagttcgac
      481 gcggatgcgg tgttcgccgc ctgcgccgag cacggtgttg cggtagagat caactgccgc
      541 cctgagcggc tggacccgcc caaacggctg ttacggctgg ccgtcgaggc gggctgcctg
      601 gtggccatcg actccgacgc gcacgccccc gggcagctcg actggctggc ctacggttgt
      661 gaacgcgccg tcgcctgcgg ggtgcccgct gaccgagtgg tgaacacctg gcctcgcggc
      721 aagctaagga actggactgc ccgttaagcc cgcgtgtccg cacaccatgc acggtgtgtc
      781 cgcatgccat gcacggtggg tccggatact gtgcgcgccg atgtgcgagt gtcagccttt
      841 cagggccttc gcggcggcaa tgagcagcgc ttcccgatca gcgatcttga tgcgagtgcg
      901 tccagccgca accccgagcg cttgctcggc agcgtcgatg gcctcccagc cggcccaggt
      961 gaccaccccg acaccccgct gggcgagttt ggcgatgatc gcgtccggct cacggtggcg
     1021 cgcgcggggc agggcctcaa cgtcggcgag gagctgctca accgtctcct tggcgtcacc
     1081 cttgttggtc ccgattactc cggtggggcc gcgtttgatc caacccacca catattcgcc
     1141 cgtcgcggcg caaccgtcgc gctgcaccag accagcaaca tgcggaatca ccccggtccg
     1201 ttcgttgaaa gggacgcccg gtagcggcag cccgcggtac cccactgagc gcagcaccag
     1261 ctgagcctcc aacgttacct tcgcaccggt gccggtggca ccaccgcagc catccaatgc
     1321 gttgcgctcg cactccaccg cctccacccg cccggcgccg tgcacggcca cgggactgag
     1381 caggaagcgc aggtggattc ggcgtggccg accctgctgc gaccgggtgg accactcttg
     1441 cagcgtctcg aggttgcgcc gcaccgtctt gttggtctcc aggattttct cgccggcgtc
     1501 gtcgagtacc agctcggcgc ggtcgaccac cacgtcggca ttggccagct cgcccagctc
     1561 ccgcagctcc ttcgtggtga acttggcctg cgccggtccg cgcctgccca cgatatagat
     1621 gtcactgatg gcgctggagt cgagcacctt cagcacgtga tcgggcatgt cggtggactg
     1681 cagctcctcg gtggtcttgg ccaggatccg ggccacgtcc acagcgacgt tgccgactcc
     1741 gaccaccacc gcgctgcgcg catccagggt gaaagcgtct acctcggcat cagggtgacc
     1801 gcagtaccag gccacgaact gggttgccga gaagctgccg ggcagatctt cgccggggat
     1861 ggacagccgg cgatccaccg acgaccccgt cgcatagatc accgcgtcgt aatactcgcg
     1921 cagctcctcc acgctgatcc cggcacccag ctcgatcccg ccgaggaacc ggaccccggg
     1981 ccgctccaga attttgcgta gggtgatctc cagcgacttc atcttcaggt ggtcgggcgc
     2041 cacgccgtag cgcaacagtc catatgggca gggcagcctg tccacgacat ccacagatac
     2101 tccgccgtgc ttgctcagct catcggcggc gtatagcccc gaggggccgg acccgatcac
     2161 cgccacccga accggtccgg tcactactca cgcacctcct gggagagcac actggcagat
     2221 cacggacccg acgttacgcc accaccgtga cgaggtggcg cagtgacgag ccggcgcagc
     2281 gcagcgacgc tcagcccatc ccgcagatgc gggtccccat gcaacaccga cactccatcc
     2341 ggacgggcca cgtcgtcgag taccgccacc gcaccggaca ggtcctggtc acgggtgtac
     2401 ccgttgacca ccgccacgca ggtcagtccc gcggcaacag cggcgtgcac accgtttgcc
     2461 gagtcctcca ctgctactgc gttggcggca ggcaggcgca gccgagccag cgcctcgagg
     2521 tagacggccg gatcgggctt gagatcagtg atatcggagc cggtgaccac gacgtcaaac
     2581 gcatcgagtc cgaaaagtat gtcgagcaat ggcagcaccc aatcgctggt gcccgtggtg
     2641 gcgaccgcag tggtgacgcc ctcggcccgc agctcac
//
LOCUS       DATADN010000323         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_639522, whole genome shotgun sequence.
ACCESSION   DATADN010000323 DATADN010000000
VERSION     DATADN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639522"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            644..1111
                     /locus_tag="VHH53_04235"
     CDS             644..1111
                     /locus_tag="VHH53_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289404.1"
                     /translation="MSRRVLRALVPLIAVLLSVSLAGPAVAATVVDVVDADRMQGGGA
                     VQVTVAVTCDPLGPDSQAFVSVNLYQGRYPRPNYIEGFGSFGEIGGISLVCDGTAHTY
                     SFTVLPSVFYADRKFRVGPARAEALVTVCTLVAPETYHCQGAGEAVEQIRIRP"
BASE COUNT          240 a          545 c          530 g          309 t
ORIGIN      
        1 cccgggggcc gcttgccgcc gtgaccacct attgtggttc agtcggcacg ctgcggggta
       61 ttggcctggc ggcggccttg ggcggtggcc gttgtgacat ggtgccgggc ccggaacagc
      121 ggtaagggct ggcccagacc ctgttcgtcc gtgcgccgcc caaggctgct gtctcgcgca
      181 atagattcag ctcgacggag ggtgaggccc gtcccgagga ccactgcctc ggtccatgag
      241 cccgtagggc tcccggcagc cgggtgggag actcgaactc cactgcgcct gtctccgcca
      301 ccagtggtca cggggctgta ggtccggtga cctgtggttc cgacgaacgt tcagtgacca
      361 ctcttgccca ccgttgtccg tcgttgccct tcggctgcgg accgagcacg gaccaacggg
      421 gctccagtcc cgtctggtcg cggacgccgt tcggtgctcc ggtcctcggc aacttggggc
      481 cgaatcggcc ggccgggccc tgaaaccggt tcggaggcgg agttccctgg tcggagttca
      541 ctagtacaac tgcagctatc gaccacccag gcgtgtctgt agcgttgcgc gcggcaccca
      601 ggtcggatgc tgctccaagc catgtggttt gaggaggtcg gtcatgtccc gccgggtcct
      661 acgtgcgctt gtcccgctta tcgccgtctt gctcagcgtg agcctcgctg gtccagcggt
      721 tgcggcgacc gtggtcgacg tcgtcgacgc agaccgcatg cagggcggcg gcgccgttca
      781 ggtcacggtc gccgtgacct gcgacccgct cgggcccgac agccaggcgt tcgtgagcgt
      841 caacctttac caaggaaggt atccccggcc taactacatc gaggggttcg ggtccttcgg
      901 tgaaatcggc gggatcagtc tcgtgtgcga cgggactgct cacacctaca gcttcacggt
      961 cctgccctcc gtgttctacg ccgacaggaa gttcagggtg ggaccggcgc gggccgaagc
     1021 actggtgacc gtctgcacct tggtcgcacc agagacgtac cactgccaag gcgctggtga
     1081 ggccgtcgag cagatccgga tccgtcccta gccgtcagcg gagtcagacc ccatccccgc
     1141 tgaccccttg catgccatag acgggatgcc ctcgcgaacg ccgcgagcac aactgtccgc
     1201 tgggcaccgc tgttgtccct tgctgtccct gccttcatgc cccagacatg ccccagggca
     1261 cctctgggtt atgagtcatt tgagcaccat tccgctgggt gctcacccgt ccgggaattt
     1321 gctatctacc ggggccgtgg ctgcttaccc acagtcagcc gtcccgccct gttcctagtg
     1381 gtgtcggcta gtctcgtgag caaaccgtga gcatccaggg tcccggcttg gtgagatggg
     1441 aggcagcggc gtgcggctag cggcatccca ggtgatcgag cgccccccgg ccgaggtgtt
     1501 ccggttcgtg gccaccgacc actttcagaa tcatcccaag tgggatccga cggtcacctc
     1561 catcaccaag acctacccgg ggccgatggg gtcggaacga ccgcgcggct ggttcggacc
     1621 gacc
//
LOCUS       DATADN010000324         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_639870, whole genome shotgun sequence.
ACCESSION   DATADN010000324 DATADN010000000
VERSION     DATADN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639870"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VHH53_04240"
     CDS             <1..795
                     /locus_tag="VHH53_04240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289405.1"
                     /translation="VVVVDNASTDGTCDAVAELFGQVTLLRSTGNLGSLGRNLAVRDI
                     TTPYVAFCDDDTWWEPGALRRAADLLDTYPGLASVTARILVAPDLVEDPITPELRDSP
                     VPGPAWLPGPALLGVLAGASMFRVDAFREVGGFSPRLWLGGEEELLAIDLAARGWWMC
                     WAEDVVIQHAPSTARDPRRRRQLGIRNTLWTAWLRRPLGSALRRTGIMLRSIPRDWAS
                     VAAVAEALRGLPWVLQERRVIPPEVERGLKLLEEPQRQSKARRYVG"
     gene            833..1126
                     /locus_tag="VHH53_04245"
     CDS             833..1126
                     /locus_tag="VHH53_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6343 family protein"
                     /protein_id="HEX2289406.1"
                     /translation="MPPQDRSRQRRTREDYERGLPGYHDPTAGFGGSTPARSALTLRA
                     VLAGFGLLFCTVAAVLMITSDVVAFGVVFAVLAVVALVDLGWVIHRKRRGEPG"
     gene            complement(1155..1838)
                     /locus_tag="VHH53_04250"
                     /pseudo
     CDS             complement(1155..1838)
                     /locus_tag="VHH53_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
     gene            1989..>2401
                     /locus_tag="VHH53_04255"
     CDS             1989..>2401
                     /locus_tag="VHH53_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289407.1"
                     /translation="MSEPLALQPKNVILCTKREREGGSRIAGAPTHLLRRDSAPVTQT
                     DNKLTNIVCKPGRSDVELHRSSGCNRTASVEQTGEQSPPLRKRGSQIDRRNVLISQQI
                     ERTQPRLDGSCIQSLSLDVKCNLGIGAGEVCGVAGT"
BASE COUNT          407 a          755 c          800 g          439 t
ORIGIN      
        1 gtggtggtgg tcgacaacgc gtcgaccgac ggcacctgcg atgcggtggc cgagctcttc
       61 ggccaggtga cgctgctgcg cagcacgggc aacctgggtt cgctgggacg caacctggct
      121 gtccgggata tcacgacgcc atacgtggcg ttctgcgacg acgacacctg gtgggaaccc
      181 ggggcgttgc gccgcgctgc cgatttgctg gacacctatc cagggctggc gtcggtgacg
      241 gcccggatcc tggtcgctcc ggacctggtc gaggatccga tcacccccga gctgcgcgac
      301 tccccggtgc ccggcccggc gtggttgccc ggtccggcgc tgctgggcgt gctggccggc
      361 gcgagtatgt tccgcgtcga tgcattccgc gaggtcggtg ggttctcacc gcggttgtgg
      421 ctcggcgggg aggaggaact gctggcgatc gatctcgccg cacgcggctg gtggatgtgc
      481 tgggccgaag atgtggtcat ccagcacgcg ccgtccacgg cgcgggatcc tcggcgccgc
      541 cggcagctcg gcatccgcaa caccctgtgg actgcgtggt tgcgccggcc gctgggcagc
      601 gcgctgcggc gcaccgggat catgctgcgc tcgatccccc gggactgggc cagcgtggca
      661 gccgtcgccg aggcgctgcg cgggttgccc tgggtactac aggaacgccg ggtgatacca
      721 cctgaggttg agcgtggcct gaagctgctc gaagagccgc aacggcaatc gaaggctcgc
      781 cggtacgtgg gctgagaccg gcgatgagcg tcgatgtgtg aaaggaagcg tcatgccacc
      841 acaagacagg tcacgccagc ggcgaacgcg ggaagactac gagcgcgggc tacccggata
      901 ccacgacccg accgccggat tcggcggttc cacgccggcc cgcagcgcgc tgaccctacg
      961 cgcggtgctg gccggtttcg ggttgttgtt ctgcaccgtc gccgcagtat taatgatcac
     1021 ttctgatgtg gtcgcgttcg gcgtggtgtt cgcggtgctc gctgtggtgg cgctggttga
     1081 tctgggttgg gtgatccacc gcaagcgccg tggtgaacca ggttgaataa cgtctgggtt
     1141 cagcttctcc agtatcaatc ctgggccatg tcgaagaagc ggctgtagtg cagctggtgg
     1201 gcaacggtca cggtggtggt cggcccgttg cggtgcttgg cgaggatcaa atccgcctca
     1261 ccggcgcgag ggtcgtcacg ctcccacgca tcgggccggt ggatcagcat caccatatcc
     1321 gcatcctgct ccaaagatcc actttcacgc aggtcggcca gcatcggcct cttgtcggtg
     1381 cgctgctctg gcccgcggtt gagctgcgaa attgtgacca ccgggacttc caactccttg
     1441 gccagcagct tgagatgccg ggagaattcg ctgacctcct gctggcggga ctcgaccttc
     1501 ttgcccgagg tcatcagctg catgtagtcc acgacgatca ggcgcaggtc atgacgctgc
     1561 ttgagccgcc gggccttggc ccggatttcc atcatcgtca tgttcggtga gtcatcgatg
     1621 aatagcgggg cctcgctgac ctcgcccatc cgtcgggcca accgggtcca gtcatcgtcg
     1681 ctcatccggc cggagcgcat gtccgccaac cggatcctgg cctcagcgga cagcagccgc
     1741 atcacgatct ctgtgcggct catctccagc gaaaagatca cgctggtcat gccatgggca
     1801 accgagcagg accgggcgaa atccagtccc agggtcgagt tgtgggtggg aatcatcgat
     1861 tccccggcca gataaaggtg gtcgctgttg tccacctgca cacagcggac gggaacggac
     1921 tgaaccggcc gcacgtcaac gatatagcgc gcgcctgatc tagcccgtga ggagtgccga
     1981 cgttctttgt gagcgagccg cttgcgctcc aaccgaaaaa cgtcatcctc tgtactaaac
     2041 gtgagcgtga aggcggtagc cgaattgctg gtgcgcccac ccacctgctt cgtcgagatt
     2101 ccgcaccggt aacccagact gacaacaagc tcacgaacat cgtgtgcaag ccgggcagaa
     2161 gtgacgtaga actgcaccgc tcctccgggt gtaaccgtac cgccagtgtc gagcagaccg
     2221 gcgagcagag cccgccgctg cgcaagcgag gctcgcagat agatcgacgg aacgtgctta
     2281 tttcccaaca aatcgagcgt acgcagccgc gcctggacgg cagttgcatc caatccctca
     2341 gcctcgacgt gaaatgcaat ctcgggatcg gcgctggcga agtttgcggc gtagctggaa
     2401 c
//
LOCUS       DATADN010000325         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_345782, whole genome shotgun sequence.
ACCESSION   DATADN010000325 DATADN010000000
VERSION     DATADN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345782"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..>1615
                     /locus_tag="VHH53_04260"
     CDS             151..>1615
                     /locus_tag="VHH53_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX2289408.1"
                     /translation="MNGLDWAVLIGYFGVMVAIGVWSHKRVDNVSDFFTAGGKMPWWL
                     SGISHHMSGYSAVMFTGYAGIAYTYGVTSFVTWSFPIALGIAIGSKLFAPRINRLRSR
                     LHVASPLEYLKNRYDLKTQQALAWSGMLLKIVDVGAKWAAIATLLSVFTGISLNQGIL
                     ITGSITAIYCTIGGLWADALTELGQFIIQLLAGIAMFVAVFVRLDDHGGFFGVWDSPE
                     LQGHEKPLVGPYGTVFLLAFLFIKLFEYNGGMLNQAQRYMATPNAKEAERSARLWAIL
                     WLVWPLVLFFPMWMSPLLVESQKADGSDSYALMTEQLLPHGLLGLVIVGFFSHTMAMC
                     SSDANAIAAVFTRDCAPVIWARARTWNQSQGLRVARIATVVFLGLSMAAATQVNSPAF
                     KDIITVVIKWVAGLMGPMAIPMMLGLLRAFRRPGPAAALVSWSTSLLAFWLVNYPIHW
                     NVDGGVPLQYQVSVPLAVSLILYILIGFVKPEDTPERL"
BASE COUNT          241 a          556 c          505 g          313 t
ORIGIN      
        1 cggttcgcgg gcggttcacg tacataaaca caagtcatct caccgaactt ttttacggtc
       61 tcttgacctt cctcttcctt gccgtgcgca cgtcaccgga accagagttc acatgcctga
      121 ccctggcggc gcacccgtgg aaggggactc atgaacggtc tcgactgggc cgtgctcatc
      181 ggctacttcg gcgtgatggt cgcgatcggc gtctggtcac acaaacgcgt ggacaacgtc
      241 agcgacttct tcacggccgg cggcaagatg ccgtggtggc tctccggcat ctcccaccac
      301 atgtcgggct acagcgcggt gatgttcacg ggctacgcgg gcatcgccta cacctacggc
      361 gtcacctctt tcgtcacctg gtccttcccc atcgccctcg gcatcgccat cgggtcgaag
      421 ctgttcgcgc cgcgcatcaa ccggctgcgc tcccggctcc atgtggcctc cccgctggag
      481 tacctgaaga accgctacga cctcaagacc cagcaggcgc tggcctggtc cggcatgctg
      541 ctgaagatcg tggacgtggg cgcgaagtgg gcggcgatcg cgacgctgct gtccgtcttc
      601 acggggatct ccctcaacca gggcatcctc atcaccggca gcatcacggc gatctactgc
      661 acgatcggcg gcctgtgggc ggacgcgctg accgaactcg gccagttcat catccagttg
      721 ctggccggca tcgcgatgtt cgtggcggtg ttcgtacgcc tcgacgacca cggcggcttc
      781 ttcggcgtct gggactcccc cgagctccag ggccacgaga aaccgctggt cggcccgtac
      841 ggcacggtct tcctgctggc gttcctcttc atcaagctct tcgagtacaa cggcggcatg
      901 ctcaaccagg cgcagcgcta catggccacg ccgaacgcca aggaggccga gcggtcggcc
      961 cggctgtggg ccatcctctg gctggtctgg ccgctggtgc tgttcttccc catgtggatg
     1021 tccccgctgc tggtcgagtc gcagaaggcc gacggctccg actcctacgc cctgatgacc
     1081 gaacagctcc tcccccacgg cctgctgggt ctggtcatcg tcggcttctt ctcgcacacc
     1141 atggccatgt gctcctcgga cgccaacgcc atcgcggccg tgttcacccg ggactgcgcg
     1201 ccggtgatct gggcgcgggc acggacctgg aaccagagcc agggcctgcg agtcgcccgg
     1261 atcgcgacgg tggtcttcct cggtctgtcg atggcggcgg cgacgcaggt caactccccc
     1321 gcgttcaagg acatcatcac ggtcgtcatc aagtgggtgg ccggtctgat gggcccgatg
     1381 gccatcccga tgatgctggg cctgctgcgg gccttcagga ggccgggccc cgcggcggca
     1441 ctcgtcagct ggtcgacgag cctgctcgcc ttctggctgg tgaactaccc gatccactgg
     1501 aacgtcgacg gcggtgtgcc actgcagtac caggtgtcgg tgccgctggc ggtctcgctg
     1561 atcctgtaca tcctgatcgg gttcgtgaag ccggaggaca ccccggagcg gctgg
//
LOCUS       DATADN010000326         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_948606, whole genome shotgun sequence.
ACCESSION   DATADN010000326 DATADN010000000
VERSION     DATADN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948606"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VHH53_04265"
     CDS             complement(<1..211)
                     /locus_tag="VHH53_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX secretion-associated protein EspG"
                     /protein_id="HEX2289409.1"
                     /translation="MRGITVELTHEEYRTIWQALGLGTRHWNMELPGLPELTQHERRA
                     QVGRTLDGLRARGLADHRSLAPMLED"
     gene            complement(225..1472)
                     /locus_tag="VHH53_04270"
     CDS             complement(225..1472)
                     /locus_tag="VHH53_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPE domain-containing protein"
                     /protein_id="HEX2289410.1"
                     /translation="MGTHFNYDDTGHQVIFDRINGGVGSGSLQEASQAWRRLGDDVGA
                     IGKSYVHSGLQGILASRQGAAADAAAAVTSAMLPWMDDVAQIATTAAQRAQGQADYWV
                     TAKHNVPPVPPAPQSASFFGDPTEWMAQKMDWIPGLTTEEEKAQQRQQDAAEQARQAM
                     RVYQSSSNGNVDLTPAFTAPHALNASIGAPPLTDARVDGAGSAAPASTGGSVGHHAAA
                     HQPAAHPMAAYQPVGTTAQLAEGAPAAPGNVIPGSSQWAGSPTPSQGMAHGAVPVGTG
                     LVASTAGQGAARSGQVSGVPGALFGRGASGVSGASGVGGASRSGAGFGPKPSVGAAQF
                     GPRPDISPHTAPEGVSGARGAPTPTGAMRGAGYGEPFVGAAGQRGGEDREHRSKYLVH
                     DDSNAIVGDLPPTAPPVIGADPG"
     gene            complement(1485..>1750)
                     /locus_tag="VHH53_04275"
     CDS             complement(1485..>1750)
                     /locus_tag="VHH53_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289411.1"
                     /translation="NNPTLEVAYGKREGLPFFELPEIAGNPAIVTRTNADIPSCDIDV
                     KTAERQSVTVIYDSKEFDSNPQQSCEVGKQVAAAVLMNLPLKG"
BASE COUNT          241 a          615 c          563 g          331 t
ORIGIN      
        1 ggtcctccag catcggcgcc aggcttcggt gatcggccaa cccacgggcc cgcaggccgt
       61 cgagcgtgcg tccgacctga gcccgccgct cgtgttgggt cagctctgga agtcccggca
      121 gctccatgtt ccagtgccgg gtgcccaggc ccagggcctg ccagatcgtg cggtactcct
      181 cgtgggtcag ctccaccgta atgccacgca aggcgataac tctcctagcc tggatccgca
      241 ccgatcaccg gcggcgcggt gggcggcagg tcaccgacga tcgcattcga gtcgtcgtga
      301 acgaggtact tgctgcggtg ctcacgatcc tctccaccgc gttggccggc ggccccgacg
      361 aaaggctccc cgtacccagc accgcgcatc gcaccggtgg gggttggtgc gcctcgcgca
      421 ccgctcactc cctcgggcgc ggtatgagga ctgatgtcgg gccgggggcc gaactgagcg
      481 gcaccgacgc tgggctttgg tccgaatccg gctccactcc tgctggcccc gccgactcca
      541 ctcgccccgc tgaccccgct ggccccgcgt ccgaacagtg ctcccggcac cccacttacc
      601 tggcctgatc ttgccgcacc ttgtccggca gtgctggcga ccaagccggt gccaacgggc
      661 actgcaccgt gtgccatgcc ctggctcggt gtcgggctgc cggcccattg gctcgatccc
      721 ggtatgacgt tgcccggtgc ggcgggcgca ccctcggcga gctgtgccgt tgtgcccacc
      781 ggctggtagg cggccatcgg atgggctgct ggttggtgag ctgccgcgtg gtgaccgacg
      841 ctgccacccg tcgacgcggg tgcagcgcta ccggcgccat ccaccctggc gtcggtcagc
      901 ggtggggccc cgatgctggc gttcaatgcg tgcggcgcgg tgaaggccgg tgtgagatcg
      961 acattgccgt tggagctgga ctggtatacc cgcatcgcct gacgggcctg ctccgcagcg
     1021 tcctgctggc gctgctgggc cttctcctcc tcggtggtca gccccggaat ccagtccatc
     1081 ttctgggcca tccactcagt gggatcaccg aagaagctgg ctgattgtgg cgctggtggg
     1141 accggtggga cgttgtgttt ggcggtgacc cagtagtcgg cctggccttg cgcccgctgc
     1201 gctgccgtgg tcgcgatctg cgcgacgtca tccatccacg gcaacatcgc gctggtgacc
     1261 gcggccgctg cgtccgccgc tgcgccctgc cgggaggcga gaatgccctg gagcccgctg
     1321 tgcacgtacg acttcccgat cgccccgaca tcgtcaccca gccgccgcca cgcctggctg
     1381 gcctcctgca gcgaacccga ccccaccccg ccgttgatgc ggtcgaaaat gacctgatgg
     1441 ccggtatcgt cgtagttgaa gtgcgtcccc atcgctgcgt cccctcaacc cttcagcggc
     1501 agattcatca gcaccgccgc ggcaacctgc ttgcccacct cacacgactg ctgcggattg
     1561 ctgtcgaact ccttcgagtc atagatcacc gtcacgcttt ggcgctccgc agttttgacg
     1621 tcaatgtcgc agctcgggat gtcggcgttt gttctcgtca cgatggcggg attaccagca
     1681 atttcaggga gttcgaaaaa tggaaggccc tcacgcttgc cgtaggcaac ttctaaggtg
     1741 ggattgttgg
//
LOCUS       DATADN010000327         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_948651, whole genome shotgun sequence.
ACCESSION   DATADN010000327 DATADN010000000
VERSION     DATADN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948651"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VHH53_04280"
     CDS             complement(<1..602)
                     /locus_tag="VHH53_04280"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX2289412.1"
                     /translation="MGPYEWAWSLHPSVLIGTGLLGALYFWGIGPLRRRRALGPPAAP
                     WQVASFCAGLLVLLLSLNGPMHDLSDYYLFSAHMLQHLLLTLVLPPLLIAGTPGWLLR
                     PLLRYPAVRTVGRVLTRPVVAATLYSVTIAVWHLAPHYDLMMRSHDVHVVTHLMFMVT
                     ATLMWWPVMSQVPELPALPPGLGMLYLFLVGIPMQLVGAL"
     gene            complement(647..997)
                     /locus_tag="VHH53_04285"
     CDS             complement(647..997)
                     /locus_tag="VHH53_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX2289413.1"
                     /translation="MSAHVPAEEHAHPTPGTYAKIGLVLFVLTALEVGLYEVTFGSSA
                     GPFGRSLEPFFIPLLLLLSAVKFALVAMYYMHLKNDSKLFSGVFVFPLIIAAIVIVAL
                     IVLQAYHWAFARSG"
     gene            complement(1008..1631)
                     /locus_tag="VHH53_04290"
     CDS             complement(1008..1631)
                     /locus_tag="VHH53_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX2289414.1"
                     /translation="MTDHAHALETTTGLDSRKLAIWTFIGSECMFFATLISNFLVNKG
                     KNLVGPFPHEPWTDPATNQVMEPIIEIPLVTFGTALLLFSSLFIVLALYGAQRGNRKM
                     LVGWLAATLVCGIFFVGMQVYEFTHFVHKGLTLSRNMFGASFYVLTGFHGTHVTIGVT
                     WLLTMFILAVRGKLTPDKAVNLEIAALYWHFVDVVWIVIFPVVYLIR"
     gene            complement(1633..>2004)
                     /locus_tag="VHH53_04295"
     CDS             complement(1633..>2004)
                     /locus_tag="VHH53_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289415.1"
                     /translation="APADPWNGATLEWAIPSPPPEWNFERVPTVDGRDPLWEEKRKRG
                     GRLAEPVASNGAGIHLPNPSYWPLVTAVGVTAVFVAIMMSPKWGPWGIVAAVALLFFG
                     LYSWLFEKGYSEFRAPSHGGH"
BASE COUNT          379 a          653 c          698 g          274 t
ORIGIN      
        1 atcagcgcgc ccaccagctg catcggaatg cctaccagga acaggtacag catgccgagc
       61 cccggaggca gagctggaag ctcgggtacc tggctcatca ccggccacca catcagggtc
      121 gcagtcacca tgaacatgag atgggtgacg acgtgcacgt cgtggctccg catcatgagg
      181 tcgtagtgcg gcgcgagatg ccacaccgcg atcgtcacgc tgtacagcgt cgccgcgacg
      241 accggacggg tcaggacccg cccgacggtc cgcacggccg gataccggag gagcggccgc
      301 agcagccatc ccggcgtgcc cgcgatgagc agcgggggta gcaccagcgt gagcagcagg
      361 tgctgcagca tgtgggcgga gaagagatag tagtcgctca ggtcgtgcat cgggccgttg
      421 agcgacagca gcagcaccag gagcccggcg cagaaggagg cgacctgcca gggcgcggcc
      481 gggggtccga gcgcccggcg ccggcggagc ggcccgatgc cccaaaagta caaggcaccg
      541 aggagcccgg tgcctatcag gacgctgggg tgcagcgacc aggcccactc gtacggcccc
      601 atcggccgcg ctcgctcgtc cgggtggacg aggagcagcg gcagtatcag cccgagcgcg
      661 cgaatgccca gtggtacgcc tggaggacga tgagggccac gatcacgatg gccgcgatga
      721 tgagcgggaa gacgaacacg ccgctgaaga gcttgctgtc gttcttcagg tgcatgtagt
      781 acatcgccac cagggcgaac ttcaccgcgg agagcagcag caggagcggg atgaagaagg
      841 gttcgaggga ccggccgaac ggccccgcgc tcgagccgaa ggtcacctcg tacagcccga
      901 cctcgagggc cgtcagcacg aacaggacca gcccgatctt ggcgtaggtg ccgggggtcg
      961 ggtgggcgtg ctcctcggcc ggtacgtgcg cgctcatcgc tcggtcctca cctgatcagg
     1021 tagacgacgg ggaagatcac gatccacacc acgtccacga agtgccagta cagcgccgcg
     1081 atctcgagat tgacggcctt gtccggcgtc agcttcccgc ggacggcgag gatgaacatc
     1141 gtgagcagcc aggtcacgcc gatggtgacg tgggtgccat ggaagcccgt caggacgtag
     1201 aagctcgccc cgaacatgtt gcgcgagagc gtgagcccct tgtgcacgaa gtgggtgaac
     1261 tcgtacacct gcatgcccac gaagaagatc ccgcagacca gggtggccgc gagccacccc
     1321 accagcatct tgcggttgcc ccgctgggcg ccgtacagcg ccagcacgat gaacagggag
     1381 ctgaacagga gcagggcggt gccgaacgtc accagcggga tctcgatgat cggctccatc
     1441 acctggttcg tcgccggatc ggtccacggc tcgtggggga acgggcccac caggttcttg
     1501 cccttgttca ccaggaagtt ggagatcagg gtcgcgaaga acatgcattc ggacccgatg
     1561 aaggtccaaa tggcgagctt gcggctgtcg agcccggtgg tggtctcgag ggcgtgcgcg
     1621 tggtccgtca ccttagtgcc ctccgtggga cggggcccgg aactccgagt agcccttctc
     1681 gaacagccag ctgtagagcc cgaagaagag cagcgccacc gcggcgacga tgccccacgg
     1741 tccccacttg ggcgacatca tgatggcgac gaacacggcg gtcacgccga ccgccgtcac
     1801 cagcggccag taggacgggt tgggcaggtg gatgcccgcg ccgttgctcg ccaccggctc
     1861 ggcgaggcgg ccgccgcgct tccgcttctc ctcccacagg gggtcgcgcc catccaccgt
     1921 cggcacgcgc tcgaagttcc actccggcgg cggcgaggga atggcccact ccagcgtggc
     1981 cccgttccac gggtccgccg gcgc
//
LOCUS       DATADN010000328         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1399097, whole genome shotgun sequence.
ACCESSION   DATADN010000328 DATADN010000000
VERSION     DATADN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399097"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VHH53_04300"
     CDS             complement(<1..397)
                     /locus_tag="VHH53_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX2289416.1"
                     /translation="MVQVCLNGPRTAADGTAVPLTPESMADSAADAVAAGATDIHVHP
                     KTPCGRDTLSPRVLAQTLSAIRARVSVPVGVTTGAWAEPDTAARLERVRSWTVLPDHA
                     SVNWHEPGAEDVAALLLDLGVGVEAGIWSG"
     gene            complement(491..1093)
                     /locus_tag="VHH53_04305"
     CDS             complement(491..1093)
                     /locus_tag="VHH53_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289417.1"
                     /translation="MAGFRSLARQVRDPRCDLALRRYSLRKCLERFAPYGHRATWDHL
                     CSRAGFGPEDRSPDPARLVAALDELEEARAVWLAYEVEFAERRKKEKHDGLRRPGSVD
                     DWHRLTWGGFGVAWCDDPAVHPREPLAEVLRRLIAALEREPGSTCPVCGGEQLAWKYD
                     LDHEPSAGPVCTDCGILVPRPVLTPEALADARRARLLVSA"
     gene            1357..>1711
                     /locus_tag="VHH53_04310"
     CDS             1357..>1711
                     /locus_tag="VHH53_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289418.1"
                     /translation="MPRRNPRTVLDRMRTPRGIPGFLKTASVCALIAGLLSPLSQAAA
                     SETETAQTTAVANDYCGNQCSDILPPGQNGNATLAQILLNQAFGTQPEHAEDQLGPYA
                     DLAKGYTGLNNDKIND"
BASE COUNT          299 a          659 c          511 g          242 t
ORIGIN      
        1 tcccggacca gatgccggcc tccacaccca ccccgagatc caggagcagg gcggcgacgt
       61 cctccgcacc gggctcatgc cagttgaccg aggcgtgatc gggcaggacg gtccagctcc
      121 gtacccgctc cagcctggcg gcggtgtccg gttcggccca cgctcccgtg gtcacaccca
      181 ccggcaccga cacccgcgcc cggatcgccg acaacgtctg agcgagcaca cgcggcgaca
      241 acgtgtccct cccgcacggt gtcttcgggt ggacatggat gtccgtggcc ccggccgcga
      301 cggcgtcggc ggcggagtcg gccatggact cgggcgtcag cggtaccgcc gtaccgtcgg
      361 cggcggtccg cggaccgttc agacaaacct gcaccatgtc ccgatggtgc cagtcaccac
      421 tgacagccgg ccccagtgac gacagtgggg gtgcggcatc cccggcgcac cccccgcgca
      481 cccgcatccg tcaagccgac accaacagcc tcgcccgcct ggcgtccgcc agggcctcgg
      541 gcgtgagcac cggacgcggc accagaatcc cgcagtccgt gcagaccgga cccgccgaag
      601 gctcgtggtc caggtcgtac ttccaggcga gttgctcccc gccacacacc gggcacgtcg
      661 agcccggttc gcgctccagc gcggcgatca gccggcgcag cacctcggcc agcggttcac
      721 ggggatggac cgccggatcg tcgcaccacg ccacgccgaa accgccccag gtcagccggt
      781 gccagtcgtc cacactgccc ggtcggcgca gtccgtcgtg cttctccttc ttgcggcgct
      841 cggcgaactc cacctcgtag gccagccaga ccgcccgcgc ctcctccagt tcgtccagtg
      901 cggccacgag ccgcgctgga tcgggggacc ggtcctcggg accgaaccct gcccgggaac
      961 acagatggtc ccaggtcgcc ctgtgcccgt aaggggcgaa cctctcaagg cacttgcgca
     1021 gcgaatagcg ccgcagtgcc agatcgcacc gcggatcgcg gacctgtctc gccagactcc
     1081 ggaaaccggc catcgtcctg cacctccgtc acacctgcac ctaattcggt cacttcggcg
     1141 tcgtcgtaag gacgtcgccg aatagacgta tcgacaagcg aatcggctcc atccgatatc
     1201 aaatggcctc cataacccgt ccgacgaggc cctgagtcga ctgccgtggc ccgtgtgcct
     1261 gttgtgctga ctccaaaaag tgacgcttgc tcatcttcaa tcccggggat accggcggta
     1321 acatcccgcc cacccccgtc gggaggagcc ccccacatgc cacggcgtaa cccacgcacc
     1381 gttctcgaca gaatgagaac tccccgcgga atccccgggt tcctgaagac cgcatcggta
     1441 tgcgcgctga tcgcaggtct tttgtcgccg ttgtcacagg cggccgcatc ggagaccgag
     1501 accgcgcaga ccacggcggt ggcgaacgac tactgcggca accagtgttc ggacatcctc
     1561 ccgcccggcc agaacggcaa cgccaccctc gcgcagatcc tcctgaacca ggctttcggc
     1621 acccagcccg agcacgccga ggaccagctc gggccgtatg ccgacctcgc caaggggtac
     1681 accggcctca acaacgacaa gatcaacgac t
//
LOCUS       DATADN010000329         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_640229, whole genome shotgun sequence.
ACCESSION   DATADN010000329 DATADN010000000
VERSION     DATADN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640229"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1745)
                     /locus_tag="VHH53_04315"
     CDS             complement(<1..>1745)
                     /locus_tag="VHH53_04315"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX2289419.1"
                     /translation="LLSWLAFRWRDHIGEGKDDNLLVLMVFVLALSVGNHLMAFLAAP
                     ALFIFLVLVKWQSLANWKLYPLMAGVAVAGLCVQLYLPIRANQDPVINEAAPKCADAV
                     SAFTNVVTMGKVAGECPELGASLRREQYQKPPLGERQAPFGAQMANFFQYFDWQWSRS
                     LQGRQGYFAGLRMPFTLLFLALGLFGAFEHYARDRKSFWYVGVLFFTLSVGLVYYMNF
                     KYGYVQQQVNGYPDSEVRERDYFYVITFSVWGLWVGVGLTSLWLRLVQGLGNRPRMLA
                     MASPVMALALIPLFTNWKYATRAGDYAARDFAYNVLQSVEPYGVLITNGDNDTFPLWY
                     LQEVEGIRRDVTVIVMSYFNTPWYVKQLRDITRPCPTPDAALRDPTRIICQRPFEPGK
                     APRFYGTPRAPTKPILTLSDVQIEQITNAAGMLSEEGMGFTARGMDFVLPPNQAIYPS
                     HQFVTRMIIDIWGDRPIYFAATTNTHLELGLVQQTSRQGLAYKLVGPDETAGLLPMPA
                     DPTILQFTGGYFDVRRNQELLDRTFMFRNMPDRAVWSDDATRNIPMQYYYAYAAMATV
                     ADMQQDRAAVERYTR"
BASE COUNT          291 a          593 c          592 g          269 t
ORIGIN      
        1 gcgggtgtag cgctccaccg cggcgcggtc ctgctgcatg tcggccaccg tggccatggc
       61 ggcgtaggcg tagtagtact gcatggggat gttgcgcgtg gcgtcgtcgg accacacggc
      121 gcggtccggc atgttgcgga acatgaaggt gcggtccagc agctcctggt tgcgccgcac
      181 gtcgaagtag ccgccggtga actgcaggat ggtggggtcc gccggcatcg gcagcagccc
      241 cgccgtctcg tcgggcccca ccagcttgta ggcgagcccc tgccgcgacg tctgctgcac
      301 cagccccagc tccaggtgcg tgttggtggt cgcggcgaag tagatgggcc ggtcgcccca
      361 gatgtcgatg atcatccgcg tcacgaactg gtgcgacggg tagatcgcct ggttgggcgg
      421 cagcacgaag tccatccccc gcgccgtaaa gcccatcccc tcctccgaca gcatccccgc
      481 cgcgttggtg atctgctcga tctgcacgtc gctcagcgtc aggatcggct tcgtcggcgc
      541 gcgcggggtg ccgtagaagc gcggcgcctt gccgggctcg aaggggcgct ggcagatgat
      601 gcgcgtgggg tcccgcagcg ccgcgtcggg cgtggggcag gggcgggtga tgtcgcgcag
      661 ctgctttacg taccaggggg tgttgaagta cgacatcacg atcaccgtga cgtcgcggcg
      721 aatgccctcc acctcctgca ggtaccagag cgggaaggtg tcgttgtcgc cgttggtgat
      781 cagcaccccg tacggctcca ccgactgcag cacgttgtag gcgaagtcgc gcgccgcgta
      841 gtcgcccgcg cgggtggcgt acttccagtt ggtgaacagg gggatgagcg ccagcgccat
      901 caccgggctg gccatcgcca gcatccgcgg ccggttgccc agcccctgca ccagccgcag
      961 ccagagcgac gtaaggccca cgcccaccca caggccccat acggagaagg tgatgacgta
     1021 gaagtagtcg cgctcgcgca cctcgctgtc cgggtagccg ttcacctgct gctgaacgta
     1081 cccgtacttg aagttcatgt agtagacgag gccgaccgag agcgtgaaga acaggacgcc
     1141 cacgtaccag aagctcttcc ggtcccgcgc gtagtgctcg aacgccccga acagccccag
     1201 cgccaggaag agcagggtga agggcatccg cagccccgcg aagtagccct ggcgcccctg
     1261 cagcgagcgg gaccactgcc agtcgaagta ctggaaaaag ttggccatct gcgcgccgaa
     1321 cggcgcctgc cgctcgccca gcggcggctt ctggtactgc tcgcggcgca gcgaagcgcc
     1381 cagctccggg cactcgcccg ccaccttgcc catcgtcacc acgttggtga acgcgctcac
     1441 cgcgtccgcg cacttgggcg cggcctcgtt gatgaccggg tcctggttgg cgcggatggg
     1501 caggtacagc tgcacgcaca gccccgccac ggccacgccg gccatcagcg ggtacagctt
     1561 ccagttggcc agcgactgcc acttcaccag caccaggaag atgaagagcg ccggggcggc
     1621 caggaacgcc atcaggtggt tgcccaccga gagcgccagc acgaacacca ttagcaccag
     1681 caggttgtcg tccttcccct cgccgatgtg gtcgcgccag cggaacgcca gccaggacag
     1741 cagtg
//
LOCUS       DATADN010000330         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_346129, whole genome shotgun sequence.
ACCESSION   DATADN010000330 DATADN010000000
VERSION     DATADN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346129"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..2719
                     /locus_tag="VHH53_04320"
     CDS             206..2719
                     /locus_tag="VHH53_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEX2289420.1"
                     /translation="MTLTEALPGDSGPDELLDTFVNWVSGQGLTLYPAQEDALIEIVS
                     GSNVILATPTGSGKSLVATGAHAVALAQGRRSVYTAPIKALVSEKFFALCNVFGAEQV
                     GMLTGDSSVNAGAPIICCTAEVLANMALRTGADTDAGLVVMDELQLYADPDRGWAWQV
                     PLLELPRAQFLLMSATLGDVTHFEQDLTRRTGRPTAVVRSAQRPVPLHFRYVTTPLHE
                     TLEELLATKAAPVYVVHFTQASAAEQAQALTSINVCSRAEKDAIAELIGGFRFATGFG
                     RTLSRLVRHGIGVHHAGMLPRYRRLVERLAQAGLLKIICGTDTLGVGINVPIRTVLLT
                     ALGKYDGTRTRHLTAREFHQIAGRAGRAGYDTAGDVVVQAPDHVVENTKALAKAGDDP
                     RKLRKVVRKKPPEGSVSWSDRTFDRLVAAEPEPLTSSFAVSHAMLLNVANRPGDAFAA
                     MRHLLTDNHEDRPAQRRHIRQAIAMYRALLAAGVVERLEVPDAQDRTVRLTGDLQLDF
                     ALNQALSPFALAVIELLDPDSVDYPLDVLSVLEATLDDPRQVLGAQLSKAKGQAVAEM
                     KAEGIEYEERMRLLEDVTYPMPLAELLHAALDTYQRGHPWVADHRLSPKSVARDLAER
                     AMTFTEYVGFYGLARSEGLVLRYLADAYQALRRTVPEEAKTDGLRDLQEWLGELVRQV
                     DSSLLDEWERLRNPETPTSAAPIAASVDHRPPPVTANVRAFRVLVRNALFRRVELAAH
                     RRWEELGELDSDAGWDAQSWRQAVEPYFDDHDEIGTGADARGPHLLIVDHQPGKWVVR
                     QILDDPAGHHDWGISAEVDLAASDGSGTAVLRVTAVGRL"
     gene            complement(2730..>3024)
                     /locus_tag="VHH53_04325"
     CDS             complement(2730..>3024)
                     /locus_tag="VHH53_04325"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEX2289421.1"
                     /translation="RTAAQVDLAIEALVSATGRPLNPPARPEHCTILGLCSTPRSVAE
                     LAALLKMPLGVARVMLGDLAAAGSVSVHRTASSGQATPDVVLMQRVLTGLRRL"
BASE COUNT          502 a         1024 c         1013 g          485 t
ORIGIN      
        1 atgcccggca atcagttgac ccgtgtcgat gagtgatgct ccccgtctgt attggcccgt
       61 ctgcactggc acgtgtgtac tggcacatct acctgcactg gccccgaccg ggggccaagg
      121 ttaccgcgcc ggctacgggg atccccgccc accggcggcc ccgccgcgcc gctcgcggac
      181 agccgcaggc acgatcgtgg ctatcatgac gctcaccgaa gcgctgcccg gcgattccgg
      241 gcccgacgag ctgctcgaca cctttgtgaa ctgggtttcg ggccagggct tgacgctgta
      301 cccggcccaa gaggacgcgc tgatcgagat tgtctcaggc tcgaacgtga tcctggccac
      361 tcctaccggc tccggtaaga gcctggttgc gactggcgcg cacgcagtcg cgctggcgca
      421 aggcaggcga agcgtctaca ccgcaccgat caaggcactg gtttcggaga agttcttcgc
      481 gttgtgcaac gttttcggcg ccgagcaggt cgggatgctg accggtgact ccagtgtcaa
      541 cgccggggcg ccgatcatct gctgcaccgc tgaggtgctg gccaacatgg ccctgcgcac
      601 cggggcggat accgacgccg gcctggtcgt gatggacgag ttacaattat acgccgatcc
      661 tgatcgcggc tgggcttggc aggtgccgct gctggagctt ccacgggccc agttcctgct
      721 catgtctgcg acgttgggtg atgtcaccca cttcgagcag gacttgaccc ggcgcaccgg
      781 ccgtcctacc gcggtggtgc gctccgcgca gcgcccggtc ccgctgcatt tccggtatgt
      841 caccacccca ctgcacgaga cactggaaga gctgctggcc accaaggcgg caccggtgta
      901 cgtggtgcac ttcactcaag cctccgctgc cgaacaggca caagcgctga ccagcatcaa
      961 tgtctgcagt agagccgaga aggacgcgat cgcggagctg atcggcggct tccggttcgc
     1021 caccggtttc gggcggacgc tgtcccggct ggtccgccac gggatcggcg tgcaccacgc
     1081 cggaatgctg ccgcggtacc gccggctcgt cgagcgactt gcccaggccg gcctgctgaa
     1141 gatcatctgc ggcacagaca ccctcggcgt agggatcaac gtgccaatcc gcaccgtgct
     1201 gctcaccgcg ctgggcaagt acgacgggac ccgcacccgt cacctcaccg ctcgggagtt
     1261 ccaccagatc gccggtcgcg ccggacgggc cggctacgac accgccggtg atgtcgtcgt
     1321 gcaggctcca gaccacgtgg tggagaacac caaggcgttg gccaaggccg gggacgaccc
     1381 gcgcaagttg cgcaaggtgg tgcgcaagaa accccctgag ggatcggtgt cctggagtga
     1441 ccgcaccttc gaccggctgg ttgcggccga gccggaaccg ctgacatcca gctttgcggt
     1501 gagccacgcg atgctgctca acgtggccaa ccggcccggg gacgcattcg cggccatgcg
     1561 ccatctgctc accgacaacc acgaggaccg gcccgctcag cgacggcata tccgccaagc
     1621 catcgcgatg taccgcgcgt tgctggccgc cggcgtcgtc gaacggctgg aagtcccaga
     1681 cgcccaggac cgtaccgtgc ggctcaccgg cgacctgcag ctcgatttcg ccctcaacca
     1741 agcgctgtcc ccgttcgcgc tggctgtgat cgagctgctg gacccggact cggttgatta
     1801 cccgctggac gtgctgtccg tgctggaggc cacgttggac gatccgcggc aggtgctggg
     1861 cgcacaactg tcgaaggcca agggacaagc cgtggcggag atgaaagccg agggtatcga
     1921 atacgaggag cggatgcggt tgctggagga cgtcacctat ccgatgccgc tggccgagct
     1981 gttgcatgcc gcgctggaca cctaccagcg gggtcatcca tgggtggctg accaccggct
     2041 gtcccccaag tcggtggctc gcgatctggc cgaacgggcg atgaccttca ccgagtacgt
     2101 cgggttctac ggcctggcgc gctcagaggg cttggtactg cgctacctgg ccgacgccta
     2161 ccaggcgctg cgccgcacag tgcccgagga ggccaagacc gatgggctgc gggacctgca
     2221 ggagtggctc ggggaactgg ttcgccaggt ggactccagc ctgctggacg agtgggagcg
     2281 gctgcgcaac cccgagacgc ccacctccgc cgcacccatt gccgcatcgg tggaccaccg
     2341 tcccccaccg gtgacagcca acgtgcgggc cttccgggta ctggttcgca acgcgctgtt
     2401 ccgccgcgtg gaactcgccg cccaccggcg ctgggaggaa ctcggcgaac tggactcaga
     2461 cgccggatgg gacgcgcaat cctggcgaca agccgtggaa ccgtacttcg atgaccatga
     2521 cgagatcggc actggcgctg acgcccgcgg cccgcacctg ctgatcgttg atcatcagcc
     2581 cggcaagtgg gttgtgcggc agatcctgga cgacccggct gggcatcatg actggggcat
     2641 tagcgccgaa gtcgacctcg ccgcctccga cggatccggg acagcggtgc tgcgggtgac
     2701 cgcggtcggc cggctctgaa accgatctat cacagtcgcc gcaggccggt gagcacccgc
     2761 tgcatcaaga cgacgtccgg tgtggcctgc ccggagctgg cggtgcggtg caccgagacg
     2821 ctacccgcgg cggcgaggtc tcccagcatc acacgggcca ctcccagagg catcttcagc
     2881 aaggcggcaa gttctgccac agagcgcggc gtggagcaca gccccagaat cgtgcagtgt
     2941 tccggacggg ccggggggtt gagtgggcgg ccggttgccg agaccagggc ctcgatcgca
     3001 aggtcgacct gcgccgcggt ccgt
//
LOCUS       DATADN010000331         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1099411, whole genome shotgun sequence.
ACCESSION   DATADN010000331 DATADN010000000
VERSION     DATADN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099411"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..499)
                     /locus_tag="VHH53_04330"
     CDS             complement(116..499)
                     /locus_tag="VHH53_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2289422.1"
                     /translation="MTEDWVAVANAIKERVNELGWRQRELAQRSHVSQAIVRELQHHT
                     VERRRSARTLEALSTALGWHPQHLTAVLRGRTPPELGDPVSDNDRTVSSRLDAIDERL
                     SEITDLLHEMNTNLAAVVSHGHTGR"
     gene            665..943
                     /locus_tag="VHH53_04335"
     CDS             665..943
                     /locus_tag="VHH53_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2289423.1"
                     /translation="MSTDMPTAGPPAFYTVPEAARILRVDPATLYRAIREDAFPAVRV
                     RTRYVVPAAALHQLVTEAAGSGGCVDVASLAAERRTAREIERLSGGAR"
     gene            943..1311
                     /locus_tag="VHH53_04340"
     CDS             943..1311
                     /locus_tag="VHH53_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2289424.1"
                     /translation="MTYDDNPQYAAMAARLDRLQQVPTEVLNTVVVRRGLCLWSLWPE
                     QEPDWEDCAPSDRALAERLCERCPVIDQCLELELRTAGASTTGVWGALPEDDRRALHR
                     VWHQRRQHQGQSDQDGGARP"
     gene            1308..1637
                     /locus_tag="VHH53_04345"
     CDS             1308..1637
                     /locus_tag="VHH53_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289425.1"
                     /translation="MTVDLSPTQVIAVLGVLLALVMMWRSGTRRGRRAADAAHMASRV
                     ASLLGYVLGLAAVIVGVQWLAITYSLGSTLFWVVLAVPALITAYVTTRALAVTSLDVT
                     YRRGDRR"
BASE COUNT          275 a          538 c          545 g          301 t
ORIGIN      
        1 gtcgctgtcc ttgtctgtcg tccgctgtcg tctatcgaaa aagtgcgata acgacgacct
       61 tgtagcaatg cacttgaacg tcactcacga actgggctcc ctcccgagca cctcctcacc
      121 gaccggtgtg tccatgactg accactgcgg ccaggttggt attcatttcg tggagaagat
      181 cggtgatctc gctcagccgc tcgtcgattg cgtccagccg ggaggacaca gttctgtcgt
      241 tatcgctgac cgggtcaccc agctcgggcg gtgtgcggcc acgcagcacg gccgtgaggt
      301 gctgggggtg ccaacccagc gcagtcgaga gcgcttcgag ggttcgtgcg ctacggcgcc
      361 gttcgacggt gtggtgttgg agttcgcgga cgatcgcttg ggagacgtgc gagcgttgcg
      421 cgagctcgcg ttgacgccat ccgagttcgt tcacccgctc tttgatggcg ttggcgaccg
      481 ccacccaatc ctccgtcacg tattcctccg cgctccaccg tcgcggcgag gctagccggt
      541 ctttgcgatc tcagggcgtg acaccccgtt ttccgccgcg cccttaccta gtcgcgcgcc
      601 catatgagct gaaatcatca caatagtgat gaaatcctaa aattgagcag gagaaaagac
      661 gtcaatgagt accgatatgc ccactgccgg cccgccggcc ttctacaccg tcccggaagc
      721 cgcacggatc ttgcgcgtgg accccgccac cctttaccgg gcaatccggg aggacgcctt
      781 ccccgccgtc cgagtccgca cccgctacgt ggtgccagca gccgcactgc accaactggt
      841 gaccgaggcc gccggctcgg gcggctgcgt cgacgtcgca agcctcgccg ccgaacggcg
      901 cacagcacgc gagatcgagc ggttgagcgg gggtgcgcgg tgatgaccta cgacgacaac
      961 ccgcagtacg cggcgatggc cgcacggctc gaccgactcc agcaggttcc gaccgaggtg
     1021 ctcaacaccg ttgtcgtgcg ccgtgggctg tgcctgtgga gtctgtggcc ggagcaggaa
     1081 ccggattggg aggactgcgc ccccagtgac cgagcactcg cagaacggct ctgtgagagg
     1141 tgcccggtca tcgatcagtg cctcgagctg gagctgcgca cggccggtgc gagcaccacc
     1201 ggtgtgtggg gcgcgctgcc cgaagacgac cggcgcgcgc tgcaccgagt gtggcaccag
     1261 cgccgtcaac accagggcca gagcgatcag gacggagggg ccaggccatg acggtcgacc
     1321 tgagtccgac gcaggtgatc gccgttctag gggtgctgct ggcgctggtc atgatgtggc
     1381 gttcgggcac ccggcgcgga cgccgagcag ccgacgccgc gcacatggcc tcccgggtgg
     1441 cctcactact tgggtacgtg ctgggtctgg cagcagtgat cgtcggcgtg cagtggctcg
     1501 cgatcaccta ttcgctgggc agcaccctgt tttgggtggt gctcgccgta ccagcactga
     1561 tcaccgctta cgtgacaact cgggctctgg cggtgacctc gctggatgtc acttaccgcc
     1621 gcggggaccg cagatgaaca cacatgacgg tgcggtgat
//
LOCUS       DATADN010000332         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_640726, whole genome shotgun sequence.
ACCESSION   DATADN010000332 DATADN010000000
VERSION     DATADN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640726"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VHH53_04350"
     CDS             <1..396
                     /locus_tag="VHH53_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX2289426.1"
                     /translation="VQSTINWHQPATAEQFEHDMAAGYVSGIAMFGVVVALSLFVLFW
                     TPTGVVVPSWLVLLFLLVLMVLPVQWALRRPWTIVARTPEPADTAGEHWVGTVHGLTA
                     ARQDTRGVVHSLKERAIPDDGTGMLRQVT"
     gene            435..1289
                     /locus_tag="VHH53_04355"
     CDS             435..1289
                     /locus_tag="VHH53_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2289427.1"
                     /translation="MPELPEVEALAHHLREHAVEATVTRVDVASLTVLKTVQPPTSAL
                     HGRCVTTASRHGKFLALHTDGPVLVTHLARAGWLRWSDSLATTAPRPGKGPIALRVHL
                     HPGSGFDLTEAGTQKRLAVYVVDAIADVPGIARLGPDALALSRDELAEALRGRTERLK
                     TALTDQTLIAGVGNAYSDEVLHAARLSPYATAGRSSDDALDRLHEALQRVLHDAVQRS
                     VGQDAARLKGEKRAGLAVHARAGLPCPVCGDTVREVSFADRAFQYCPTCQTGGKPLAD
                     RRLSRLLK"
     gene            1325..>1528
                     /locus_tag="VHH53_04360"
     CDS             1325..>1528
                     /locus_tag="VHH53_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2289428.1"
                     /translation="MTELPISAARDHLGDVVAKAEHAHERTILTRHGRAVAAVIPIDD
                     LRALEAAEDEADLLAAREALAAEE"
BASE COUNT          235 a          511 c          534 g          248 t
ORIGIN      
        1 gtgcagagca cgatcaactg gcaccaaccg gcgacggcgg agcagttcga gcatgacatg
       61 gccgctggtt acgtgtccgg tatcgcgatg ttcggggtgg tcgtcgcttt aagtctcttc
      121 gtactgttct ggacgcccac cggagtcgtt gtgccgtcgt ggttggtgct gttgttcctg
      181 ctggtcctga tggtgctgcc ggtgcagtgg gcgctgcggc ggccgtggac catcgtggcg
      241 cgtacgcctg agccggccga caccgccggt gagcactggg ttggcaccgt gcacggcctg
      301 acggcggccc ggcaggacac tcgtggagtg gtccattctc tcaaggagcg ggccatcccc
      361 gacgacggta cggggatgct ccggcaagtc acgtgaccgt gcggtggagt tgcgagtgag
      421 ggcagactgg acccatgccg gagctgccgg aggtcgaggc actggcgcac cacctgcgcg
      481 agcacgccgt cgaggcgacc gtgactcgtg ttgatgtcgc ttcgctcacg gtgctcaaga
      541 ctgtgcagcc gcccacgagc gcgctgcacg gacgctgcgt caccaccgcc agcaggcacg
      601 gcaagttcct cgcgctgcac accgacgggc cggtgctggt gacccacctg gcgcgagcag
      661 gctggttgcg ctggtccgat tccctggcca cgaccgcgcc ccggccaggc aagggaccga
      721 tcgcgctgcg agtgcacctg cacccaggct ccggcttcga cctcaccgag gccgggaccc
      781 aaaaacggct ggcggtgtat gtggtcgatg cgatagccga cgtccctggc attgcccggc
      841 tcggcccgga cgccctggcg ctgtcccgcg acgagctcgc cgaggcgcta cgcggacgta
      901 ccgagcggct caagaccgcc ctgaccgacc agacattgat cgccggggtc ggcaatgcct
      961 actccgacga ggtgctgcac gccgcgaggc tgtcccccta cgccaccgcg gggcggtcgt
     1021 ccgacgacgc actggaccgg ctgcacgagg cgctgcagcg agtgttgcac gacgcggtgc
     1081 aacgctccgt tggccaggac gccgcacggc tcaaggggga gaagcgggcc ggtctggcgg
     1141 tgcacgctcg cgcgggtctg ccctgcccgg tatgcggtga caccgtacgt gaagtgtcct
     1201 tcgccgaccg cgcgttccag tactgcccga cctgccagac cgggggcaag cccctcgccg
     1261 accgccgcct gtcccggctg ctgaagtaag gctcagtcct ctacccgtat tgtacaattt
     1321 gggtatgacg gagttgccca tcagcgccgc ccgtgatcat ctgggtgatg tggtcgccaa
     1381 ggcggaacac gctcatgagc ggaccatcct gactcggcac ggccgagccg tcgccgccgt
     1441 gattccgatc gacgacctgc gagcgttgga agccgccgaa gacgaggcgg acctgctagc
     1501 ggcacgagag gcgcttgccg cagaggag
//
LOCUS       DATADN010000333         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_949442, whole genome shotgun sequence.
ACCESSION   DATADN010000333 DATADN010000000
VERSION     DATADN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949442"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..750
                     /locus_tag="VHH53_04365"
     CDS             145..750
                     /locus_tag="VHH53_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2289429.1"
                     /translation="MPTVPGEQVRPRGRRRAGEDTRAALLDAARVEFTERGFDGATVR
                     TIALRAGVDAAMVNHWFGGKDGLFVAAMEIPVNPAEIIPRILDGAPEQLAERILRTFL
                     SVWDTNGGGALAALMRSVASHEEAARMMREFVSRVVIGRIVSAVAPDRPELRASLCGT
                     QIAGLAMIRYVIRLEPLASAQHDTVVAVIAPNLQRYLTGVL"
     gene            complement(766..1116)
                     /gene="hisI"
                     /locus_tag="VHH53_04370"
     CDS             complement(766..1116)
                     /gene="hisI"
                     /locus_tag="VHH53_04370"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX2289430.1"
                     /translation="MSALDPAIAGKLKRNVDGLVCAVVQQRGTGEVLMVGWMDDEALH
                     RTLTTGRATYWSRSRQAYWIKGETSGHTQHVREVRLDCDGDALLVVVDQIGPACHTGS
                     GTCFQQVLVSGIQC"
     gene            complement(1113..1886)
                     /gene="hisF"
                     /locus_tag="VHH53_04375"
     CDS             complement(1113..1886)
                     /gene="hisF"
                     /locus_tag="VHH53_04375"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX2289431.1"
                     /translation="MAVAVRVIPCLDVDAGRVVKGVNFTALRDAGDPVELATAYDAQG
                     ADELTFLDVTASSADRETTYDVVRRTAECVFIPLTVGGGIREVSDVDRLLRAGADKVS
                     INTAAVTRPELLREASHRFGAQCIVLSVDARRVPPGEPATPSGFEVTTHGGRRGTGID
                     AVAWAARGQELGVGEVLLNSMDADGTKAGFDLEMISAVRAVVTVPVIASGGAGEVGHF
                     PPAVAAGADAVLAASVFHFGQLRIGDVKDSLRVAGVTVR"
     gene            complement(1962..>2111)
                     /locus_tag="VHH53_04380"
     CDS             complement(1962..>2111)
                     /locus_tag="VHH53_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX2289432.1"
                     /translation="PVIASGGISGAADVAALAALASDGVEGAIIGKALYTGALTLPDA
                     LAAVL"
BASE COUNT          348 a          740 c          696 g          327 t
ORIGIN      
        1 agggcccgaa actgctcccg gtccaggtgc aggtgcccga cgccgttgtt gccgatgctg
       61 accatgcggt cattgtgccc accgtttgac gccgggtgat gaccgggcat accttttctt
      121 cttccgttga aaagaggaac cggagtgccg actgtgcccg gtgagcaagt ccgtccccgt
      181 gggcgccgtc gcgccggcga ggacacccgc gcggcgttgc tcgacgctgc cagggtggag
      241 ttcacggagc gcggcttcga cggggccacc gtgcgcacga tcgccctccg cgccggggtg
      301 gacgccgcga tggtcaacca ctggttcgga ggcaaggacg ggctgttcgt cgccgccatg
      361 gagatcccgg tcaaccccgc ggagatcatc ccgcggatcc tcgacggggc acctgagcag
      421 ctcgccgagc gaattttgcg caccttcctg tccgtctggg acaccaacgg tggtggagcg
      481 ctggccgcgc tgatgcgcag cgtggccagt catgaggaag ccgcccggat gatgcgcgag
      541 ttcgtcagcc gggtggtcat cggtcggatc gtttccgcgg tcgcgccgga ccgtcccgaa
      601 ctgcgtgcct cgctgtgcgg cacccagatc gccggactgg ccatgatccg gtacgtgatt
      661 cgcctcgaac cgctggcttc agcccagcac gacacagtgg tcgccgtaat cgcccccaac
      721 ctgcagcgct atctgactgg cgtcttgtga gtgcgtaaca gtgttctagc actgaatgcc
      781 actcacgagg acctgctgaa agcaggtccc ggacccggtg tggcaggccg ggccgatctg
      841 gtcgacgacg accaacaggg cgtcaccgtc gcagtccagc cgcacctcgc gcacgtgctg
      901 ggtgtgtccg gaggtctcgc ccttgatcca ataggcctgc ctgctgcgcg accagtaggt
      961 ggcccggccg gtggtcagcg tgcggtgcag cgcctcgtcg tccatccagc cgaccatcag
     1021 cacctcgccg gtgccacgtt gctgcaccac agcgcagacc agcccgtcga cgttgcgttt
     1081 gagcttcccg gcgatggccg gatccagggc actcaccgca ctgtcacccc ggccacgcgc
     1141 aggctgtcct tgacatcacc gatccgcaac tgtccgaaat ggaacacgct ggcggcgagc
     1201 accgcgtcgg cgccggccgc caccgcgggc gggaagtggc cgacctcgcc agcgccgcca
     1261 ctggcgatca ccgggaccgt gacgacggct cgcaccgcgg agatcatctc aagatcgaag
     1321 cccgccttgg tgccgtcggc gtccatcgaa ttgagcagga cctcgcccac gcccagctcc
     1381 tggccacggg cagcccaggc cacggcatcg atcccggtgc cacgccgccc gccgtgggtg
     1441 gttacctcga agccggatgg cgttgctggt tcgccgggtg gcacccggcg agcatccacc
     1501 gacagcacga tgcattgcgc accgaagcga tggctggcct cacgcaacag ctcgggacgg
     1561 gtcaccgcgg cggtattgat gctcaccttg tctgcgcccg cccgcagcaa gcggtccaca
     1621 tcggacactt cccggattcc cccgccgacg gtgagcggaa tgaagacgca ttccgcggtg
     1681 cggcggacca cgtcgtaggt cgtctcccga tccgccgagg acgcggtgac gtcgaggaag
     1741 gtcagctcgt cggcgccctg cgcgtcataa gcggttgcca actcgaccgg atcaccggcg
     1801 tcgcgcaatg cggtgaagtt cacccctttg accacccggc cggcatcgac atctaggcac
     1861 gggatcacac gcacggcaac agccatccgc tcagcctaag cgggcgagca cagggtgcgg
     1921 acacaccggg caacagagca cgacacaccc gaggccgggg tttagagaac agcggccagc
     1981 gcgtcgggca gggtgagcgc tccggtgtag agcgccttgc cgatgatcgc gccttccacg
     2041 ccatccgagg ccagcgcggc cagggcggcc acgtcggcag ctccggagat cccaccggag
     2101 gcgatcaccg g
//
LOCUS       DATADN010000334         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_183250, whole genome shotgun sequence.
ACCESSION   DATADN010000334 DATADN010000000
VERSION     DATADN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183250"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VHH53_04385"
     CDS             <1..720
                     /locus_tag="VHH53_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX2289433.1"
                     /translation="LVYAWPWKRVMLFSELTKRTGTGLHRVHYPTIRRVRADGWRDRI
                     SVTLLHGQCAATYAAQAEELANSFAAHTCRVRVDRPRRIWLDFIHADPLAQSLIAPTL
                     ADPTSVPDLTRVPIGRTETGRLWTLRLLDRHILVAGVSDAGKSSVIWAVLRALAPWIR
                     SGLVQVFGIDPKGGMELGRVPGLFHKLVCSNGAQAVELLEQVATLTRQRAETLRTQGT
                     RKWSPGSGNRLCCSLSTSWPT"
     gene            708..1133
                     /locus_tag="VHH53_04390"
     CDS             708..1133
                     /locus_tag="VHH53_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289434.1"
                     /translation="MADVIAYQPDNTLRKRANAALQSILSQGRAPGVCVIGQIQDPRK
                     QIIDFRHLFPTKIAMRLDEPEQVDMVLGDGVRARGAAAHEISEDTPGVAWIKHDGRRD
                     PDRARAFHTTDADLDELSAYILTGWLDAPHPMTGKQALA"
     gene            1244..1750
                     /locus_tag="VHH53_04395"
                     /pseudo
     CDS             1244..1750
                     /locus_tag="VHH53_04395"
                     /inference="COORDINATES: protein motif:HMM:NF042633.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator"
     gene            complement(1790..1919)
                     /locus_tag="VHH53_04400"
                     /pseudo
     CDS             complement(1790..1919)
                     /locus_tag="VHH53_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator"
BASE COUNT          342 a          711 c          570 g          296 t
ORIGIN      
        1 ctggtctacg cctggccgtg gaagcgggtc atgctcttct ccgagctgac caagcgcacc
       61 ggcacgggcc tgcaccgggt gcactacccc accattcgac gggtgcgcgc cgatggctgg
      121 cgtgaccgaa tctcggtcac gctgctacac ggccagtgcg ccgcaaccta cgccgcccag
      181 gctgaggaac tggccaactc cttcgccgcc cacacctgcc gcgtgcgggt ggatcgtccg
      241 cggcggatct ggctggattt catccacgcc gatcccctcg cccagtccct catcgcaccc
      301 acgctggccg atcccaccag cgtgccggac ttgacccggg tgcccatcgg gcgcacggaa
      361 accggccgac tctggacact gcgtctactc gaccggcaca tcctggtcgc gggggtcagc
      421 gatgccggca agtcctcggt gatatgggcg gtgctgcgcg ccttagcccc ctggatcaga
      481 tccgggctcg tgcaggtctt tggcatcgat cccaagggcg gtatggaact gggtcgcgta
      541 cccggactgt ttcacaagct ggtgtgcagc aacggcgcgc aggccgtgga actgctggag
      601 caggttgcca ccctgacccg ccaacgcgcc gagacccttc gcacacaggg cacccggaaa
      661 tggtcgccag gctcgggcaa ccgtttgtgt tgctcattgt cgacgagttg gccgacgtga
      721 tcgcctacca acccgacaac accctgcgca agcgcgccaa tgccgcgttg caatccatcc
      781 tgtcccaagg ccgcgcaccc ggtgtgtgcg tgatcgggca aatccaagac ccccgcaaac
      841 agattattga tttccggcac ctcttcccca ccaagatcgc catgcggctc gacgaacccg
      901 aacaagtcga catggtcctc ggcgacggtg tgcgggctcg tggcgcagca gcgcacgaaa
      961 tcagcgaaga cactcccggt gtcgcctgga tcaaacacga cggccgccgc gaccccgacc
     1021 gcgctcgcgc cttccacacc accgatgccg acctcgacga actgtccgcc tacatcctca
     1081 ccggctggct cgacgccccc caccccatga ccggaaagca ggccctggca tgaccgtgga
     1141 tcagacgtca ctgacccggg cccagcgggc cgcgctgccg ctgagtaccg acgtcgccca
     1201 cgccatcgcc gaacaacacg gcgtctgtgt ccggccgata gccatgcggc gaatcgacac
     1261 caccaccggc cgcatcgaca tcgttcccgt cccctgcgga tccacccgag aagaccgctg
     1321 cggaccatgt gcggacaagg cacgacggtt acggatggcc caatgccgcg agggctggca
     1381 cctcgaagct gagcccgtca tcgaacgggc caagcccact cgcgatcaac aggagctgat
     1441 ggctgcccgg gccgatctct tcaccgtcca cgccgaatgc cgagctgttg gtgatgacgc
     1501 ctcctgtgaa cagatcgccg acgcagtcgc cgaactcgac accgaactcc gcgccgctgg
     1561 agtacgcggc cggctgaccc cactcgatcc cccatccaag ccggtcaagc gatcgactcg
     1621 gcgccgccaa gacacccctg acttgcctcg ccgacccatc gagcggcgca ccctagggcg
     1681 ggtctacgcc gggcgctacc ggccctccac gttcctgacc ctcaccctcg attcctacgg
     1741 ccaggtggac aaccacgggg cggcggtcga cccggatcac tatgactacc ggtgcgccgc
     1801 gtttctgcgg ttctacggtg ccgaagtact gcacgtccca tccgacaaag cgccgggtgt
     1861 tttgccagaa ccggtccagc agggccggga agtggatggc gtcgcgggcg gcatgccgg
//
LOCUS       DATADN010000335         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1399812, whole genome shotgun sequence.
ACCESSION   DATADN010000335 DATADN010000000
VERSION     DATADN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399812"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="paaA"
                     /locus_tag="VHH53_04405"
     CDS             complement(<1..574)
                     /gene="paaA"
                     /locus_tag="VHH53_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX2289435.1"
                     /translation="MEQDLERDFERVVAADQRIEPRDWMPDAYRKTLIRQIAQHAHSE
                     IIGMQPEGNWISRAPSLRRKAILLAKVQDEAGHGLYLYAAAETLGISREEMYEQLLSG
                     KAKYSSIFNYPTLSWADIGAIGWLVDGAAIMNQVPLQRTSYGPYARAMVRVCKEESFH
                     QRQGFEILYTLSSGTPAQHDMAQDAVNRWWW"
     gene            complement(584..1039)
                     /locus_tag="VHH53_04410"
     CDS             complement(584..1039)
                     /locus_tag="VHH53_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX2289436.1"
                     /translation="MAVTQMRVRWIAVRAVLAVGALVTGLGLLLVIAAWTTDARIDGQ
                     TGYANAEVVSVSFQRTLVRFQTPDGAEHIPSVGVLYPEALEQGQVVQVEYDQRNPDLV
                     RVAGRGAWLTVLPVATITLATWVVLLPVAWWLRRPLARVTLDRNSSAQV"
     gene            complement(1133..>1999)
                     /locus_tag="VHH53_04415"
     CDS             complement(1133..>1999)
                     /locus_tag="VHH53_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX2289437.1"
                     /translation="NTYNTMLVVGQYDDLRFQTAPNGQPFISAYANDLGANGDAARAS
                     VERTPEVVELQETIFGPYPVEAQGGVVTNNGELGFALETQTRPVYDGLFFRRGSNTYV
                     VAHEMAHQWFGDSVAVQGWTDIWLNEGFATYAEYLWSEYLGEGTPAEIAQFTYDSIPA
                     DDEFWQVVPADPGPENQFHDSVYDRGGMTLQALRTAVGDDAFFEIVRSWADEHRFGNV
                     ATADFIAFAERISGQQLDELFEVWVYTAGKPATGPNDVVGAARAASAVPAAEPASYRK
                     IMDTHALLATQR"
BASE COUNT          295 a          713 c          675 g          316 t
ORIGIN      
        1 gccaccacca ccggttgacg gcatcctgcg ccatgtcgtg ctgagccggt gtgccggagg
       61 acagcgtgta gaggatctcg aagccctggc gctggtggaa gctctcctcc ttgcagaccc
      121 tgaccatcgc ccgcgcataa gggccgtagg aagtgcgctg aagcggcacc tggttcatga
      181 tcgccgcgcc atcgaccagc cagccgatgg cgccaatatc cgcccacgac agggtgggat
      241 agttgaagat cgacgagtac ttggcctttc cggacaagag ctgctcgtac atctcctcgc
      301 gcgagatgcc gagcgtctcg gcggcggcgt agaggtagag cccgtggccg gcctcgtcct
      361 gcaccttggc cagcaggatg gccttgcgcc gcagggaggg tgcccggctg atccagttgc
      421 cctccggctg catgccgatg atctcggagt gggcgtgctg ggcgatctgc cggatcagcg
      481 tttttcggta tgcgtccggc atccagtccc gcggttcgat gcgctgatcg gccgcgacca
      541 cgcgctcgaa gtctcgctcc aggtcctgtt ccacaccccg atgttacacc tgagcgctgg
      601 aattgcggtc gagtgttaca cgcgcgagcg ggcggcgcag ccaccaggcc acgggcagca
      661 gcaccaccca ggtggccagc gtgatggtgg cgaccgggag cacggtcagc cacgcgccgc
      721 ggccggccac ccgcaccagg tcggggttgc gctggtcgta ctccacctgc acgacctggc
      781 cctgctccag cgcctccggg tagagcacgc cgaccgacgg gatgtgctcg gcgccgtccg
      841 gtgtctggaa ccgcaccagc gtgcgctgga acgacaccga gacgacctcg gcgttggcat
      901 agccggtctg cccgtcgatc cgggcgtccg tggtccacgc cgcgatgacc agcagcagcc
      961 cgagcccggt gaccagggca ccgaccgcga gcaccgcccg caccgcgatc caccgcaccc
     1021 gcatctgcgt caccgccacc cggctgagcc tatccgctcc ggcgcgtggc acgtgccggg
     1081 ctggaccccg gcgagacgtt gagtgtcccg acgggatcca gccgggcctg ggtcaccgct
     1141 gggtggccag cagcgcgtgc gtgtccatga tcttgcggta cgacgccggc tcggccgccg
     1201 gaaccgccga cgccgcccgc gccgcgccga ccacgtcgtt cgggccggtc gccggcttgc
     1261 ccgcggtgta cacccacacc tcgaacagct cgtcgagctg ctgcccggag atccgctcgg
     1321 cgaacgcgat gaagtcggcc gtcgccacgt tgccgaaccg gtgctcgtcc gcccaggacc
     1381 gcacgatctc gaagaacgcg tcgtcgccca cggcggtgcg cagcgcctgg agggtcatgc
     1441 cgccgcggtc gtacaccgag tcgtggaact ggttctccgg cccggggtcg gccggcacga
     1501 cctgccagaa ctcgtcgtcg gccgggatcg agtcgtaggt gaactgggcg atctccgccg
     1561 gcgtgccctc gcccaggtac tccgaccaca ggtactccgc ataggtggcg aagccctcgt
     1621 tcagccagat gtcggtccag ccctgcaccg cgacggagtc gccgaaccac tggtgcgcca
     1681 tctcgtgcgc gaccacgtac gtgttggagc cgcggcggaa gaacaggccg tcgtacaccg
     1741 gcctggtctg ggtctccagt gcgaagccca gctcgccgtt gttggtgacg acgccgccct
     1801 gcgcctccac cgggtacggc ccgaagatcg tctcctggag ctcgaccacc tccggggtcc
     1861 gctccacgct ggcccgggcc gcgtcgccgt tggccccgag gtcgttggcg tacgcggaga
     1921 tgaacggctg gccgttcggc gcggtctgga acctcaggtc gtcgtactgg ccgacgacca
     1981 gcatggtgtt gtacgtgtt
//
LOCUS       DATADN010000336         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1237646, whole genome shotgun sequence.
ACCESSION   DATADN010000336 DATADN010000000
VERSION     DATADN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237646"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..864
                     /locus_tag="VHH53_04420"
     CDS             214..864
                     /locus_tag="VHH53_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX2289438.1"
                     /translation="MVPVPAGSVVLTNRRSQRSWAVDVAAHQMSAFPVTQAQYAQVTG
                     EWPSTARGDRLPVEGVSWWDAVRFCHALSQQEQLVPAYRLDPDGEGIAWDASADGYRL
                     PTEAEWEHACRAGTGAARYGELDDVAWYRGNSEGRLHSVGGKQPNAWGLFDMLGNVWE
                     WCWDVYDAEVYGTYRVLRGGGWFDEHWSCRASVRRRSHPTFRIDDVGFRVARSVSH"
     gene            1024..1176
                     /locus_tag="VHH53_04425"
     CDS             1024..1176
                     /locus_tag="VHH53_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289439.1"
                     /translation="MHGGPKGEVFGADGRTVVATVAGSDVYEELGPPIVHHVDVEIGG
                     SRPSLP"
     gene            complement(1302..2153)
                     /locus_tag="VHH53_04430"
     CDS             complement(1302..2153)
                     /locus_tag="VHH53_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2289440.1"
                     /translation="MEALHGARMLTGLVLALSASVLWGTADFAGGRLSRHLPLLIVVT
                     CSQGAGLVALLIVLLLRGGVQPAAAGWGTVAGVLSVGSVACLYRALAIGTMSVIAPIV
                     ATSAVVPVLVGLATGERPGPAAGAGIVLTLAGVVLASRQQAYHPPADHRLSVLLAVAA
                     AVCAGGQLVALQRAGSIDALSGVGASRAVSVSIFIVVLLFARTRAPVRALPAAAVVGV
                     LDTAANLAYTLATTRALLSLSAVLASLYPVVTIALARALLHERLRPLQVTGAVLVMAG
                     VLLIASR"
BASE COUNT          365 a          794 c          837 g          291 t
ORIGIN      
        1 cagccgctcg gccgggggag accagccgag ccggtgtcat ctcagggggc ggtagcgtcc
       61 cgacctcatg cggacgggtg cggagaccgt cccggagagc cccagccgcc ggcccggccc
      121 gacagccgct cggccggggg agaccagccg agccggtgtc atctcagggg gcggtagcgt
      181 ccccacctca tgaggacggg tgcggagagc gcgatggtcc ccgtcccggc cggcagcgtg
      241 gtgctgacca accgacggtc gcagcgcagc tgggcggtcg acgtcgcggc gcaccagatg
      301 tccgcctttc cggtgaccca ggcgcagtac gcgcaggtca ccggggaatg gccgagcacc
      361 gcgcgtggcg accggctgcc ggtggaggga gtttcgtggt gggacgcggt gcggttctgc
      421 catgcgctgt cccaacagga gcagctggtc cccgcctacc ggctcgatcc cgacggcgag
      481 ggcattgcat gggacgcgtc cgccgatggc tatcggcttc ctactgaggc cgagtgggag
      541 cacgcctgcc gcgccgggac cggcgccgcg cggtatgggg agctcgacga tgtcgcgtgg
      601 taccgcggca actcggaggg acgactgcac tcggtgggcg gcaagcagcc caacgcctgg
      661 gggttgttcg acatgctggg caacgtctgg gagtggtgct gggacgtcta cgacgccgag
      721 gtatacggca cctaccgagt gctccgcggc ggtggctggt tcgacgagca ctggagctgt
      781 cgggcctcgg tacggcgcag gagtcatccc accttccgga tcgacgacgt cggcttccgc
      841 gtcgcccgtt cggtttccca ttgacccgca gcggacctcc gccccctctg ggcggccgcg
      901 tccgcgtcgg tcgggtcttc gccagagggt cagcccgcac cgcactcact gaagctttga
      961 cgacgcagga ccaccaagcc gccggacagg ccgaccaggt accgacgatg gccccactga
     1021 tcggtgcatg gcggaccgaa gggcgaggtc ttcggcgcgg acggccgcac cgtggtggcg
     1081 accgtcgcgg ggagcgacgt ctacgaggag ctcgggccgc ctatcgtcca ccacgttgat
     1141 gtcgagattg gcggctcacg accttcgctg ccatgaccca ggctcttgac gacgcccgca
     1201 agaggttcct tgatcggcgc accacgattc cgcttccggg atgcgaccac cgccggctca
     1261 gcgggaaccg ggtcccggcg gccgcggccg gaatggtgcg actaccggct ggcgatgagg
     1321 agcacgcctg ccatgaccag gacggcgccg gtgacctgta gcggacgcag ccgttcatgc
     1381 agcagcgcgc gggcgagggc gatggtgacg acggggtaga gactcgccag caccgcggac
     1441 agggacagca gcgctcgggt ggtggcgagg gtgtaggcga gatttgcggc ggtgtcgagc
     1501 actccgacca ccgcggcagc aggcagcgcc cgcaccggcg cgcgggtgcg ggcgaacagc
     1561 agcacgacaa tgaagatgct tacgctgaca gcccggctcg cgccaacccc gctgagtgca
     1621 tcgatgctgc cggcgcgctg cagggcgacc agctggcccc cggcacacac cgctgcggcc
     1681 accgccagaa gcacgctgag ccggtgatcg gctggcgggt ggtaggcctg ctgcctgctg
     1741 gcgagaacca ccccggccaa cgtgagcacg atgcctgcac ccgcggctgg acccggccgc
     1801 tcacccgtgg cgaggccgac cagcacgggc accaccgcgg aggtggccac gatcggagcg
     1861 atcacgctca tcgtcccgat ggccaacgcc cggtagaggc aggcgacgct gcccacggag
     1921 agcacaccgg cgaccgtccc ccacccggcc gccgcgggct gcacgccgcc acgcaggagc
     1981 aggacgatca gcagggccac gagcccggcg ccttggctgc aggtgaccac gatcaacagc
     2041 ggcagatgcc ggctgagccg gccgccggcg aagtccgcgg tgccccacag caccgaggcg
     2101 gacagcgcca gcaccaggcc ggtgagcatc cgggcaccat gcagggcctc cacccggtga
     2161 cgccagtaca ccgggtggta ccagtactgc cagcactggt ccgtcggcgt ggacgaagct
     2221 cagcgagccg agcccggcgg gtcctggagg acatcaccga cgccaccttc ggtgtccggt
     2281 gagcgat
//
LOCUS       DATADN010000337         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1100124, whole genome shotgun sequence.
ACCESSION   DATADN010000337 DATADN010000000
VERSION     DATADN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100124"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VHH53_04435"
     CDS             complement(<1..375)
                     /locus_tag="VHH53_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2289441.1"
                     /translation="MIPTPPGADQDTPIFVISVAAELAGMHAQTLRGYDRLGLVSPGR
                     SPGGGRRYSARDIALLQEVQRLSQDHGVNLAGIKRIIDLENQVEALRSRMAELAAELD
                     EARTELARAQVVAEQAAAAVHAS"
     gene            complement(372..1553)
                     /gene="dnaJ"
                     /locus_tag="VHH53_04440"
     CDS             complement(372..1553)
                     /gene="dnaJ"
                     /locus_tag="VHH53_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX2289442.1"
                     /translation="MSAREWIEKDFYAELGVSSTASADDIKRAYRKLARKLHPDANPG
                     DSRAEARFKAVSEAYGVLSDPEKRKQYDEARSLFNGGFRPGGGFGGPAGAGGFDLGDL
                     FGSTTGRGGDAGGLGDLLGGLFANRGAGNAGASRPRRGTDVETEVRIDFTEAVHGATV
                     PLRLSSPAACGSCGGSGARAGTVPRICPVCSGSGLVTRSQGAFAFSEPCRDCRGTGRI
                     IDDPCAECGGRGVSNRHRTLTVRIPAGVADGQRIRLAGQGEPGMHGAPAGDLYVLVHV
                     TPHPVFGRSGDDLTLEVPVSFPELVRGTTLTVPTLDGTVSLKVPAGTANGRTLRVRGK
                     GITRRQGRSGDLLVTLQVEVPTKLDRAAKEALEAYAQATAGHDPRANLFARAHSQGAA
                     R"
     gene            complement(1573..>2013)
                     /locus_tag="VHH53_04445"
     CDS             complement(1573..>2013)
                     /locus_tag="VHH53_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX2289443.1"
                     /translation="DRQLTIALAKAQVASDLLTVLDDIERAQEHGDLSGAFKAVADKL
                     VAGLQAHGLEAFGAIGDGFDPAVHEAVHHDTSPDVHEPTVTAVLRRGYRLGERVLRPA
                     MVAVTDAEPVGDTSETTEPAEMSSDAAMSRDAAWSGGAEPLQEN"
BASE COUNT          316 a          751 c          652 g          294 t
ORIGIN      
        1 ggaggcgtgc acggccgcgg cggcctgctc ggcgacgacc tgcgcgcggg ccagttcggt
       61 acgcgcctca tccagttcgg cagcgagctc agccatcctg gagcgcagcg cctccacctg
      121 gttttccagg tcgataatcc gcttgatccc ggccaggttg accccatggt cctgggacag
      181 ccgctgcacc tcctgcagca gcgcgatgtc gcgcgccgaa taccggcggc caccgccagg
      241 gctacggccg ggcgacacca gacccagccg gtcatagcca cgcaaggtct gcgcgtgcat
      301 ccctgccagc tcagccgcga cggagatcac aaagatcggg gtgtcctggt ccgcgcccgg
      361 cggagtcggg atcatcgcgc cgctccctgg ctgtgtgccc gggcgaacag gttggcccgc
      421 gggtcatgcc cagcagtggc ctgcgcgtag gcctcaaggg cctctttcgc cgcccggtcc
      481 agtttggtcg gtacctcaac ctgcagagtt accagcagat cgccggaccg accctgccgc
      541 ctggtgatgc ccttaccgcg cacccgcagc gtgcgaccgt tggcggtgcc ggccggaacc
      601 ttgagcgaca cggttccgtc cagggtaggc acggtcagcg tcgtgcccct gacgagctcg
      661 ggaaacgaca ccggcacctc cagcgtgagg tcgtcaccgg aacgcccgaa caccggatgt
      721 ggagtgacgt gcaccagtac atagaggtca ccggcaggcg ctccatgcat gcccggctcg
      781 ccctgcccgg ccagccggat ccgctgacca tcggcgaccc cggcggggat ccgcacggtc
      841 agcgtgcggt gccggtttga caccccccgc ccaccacact cggcacacgg gtcatcgatg
      901 atccgccccg taccacggca gtcgcggcac ggctccgaaa aggcgaacgc tccctggctg
      961 cgggtcacca acccgctgcc gctgcagacc gggcagatcc ggggaacggt gccagcacgg
     1021 gccccggaac cgccacagct gccgcaggcg gctgggctgg acaaccgcag cggcacggtg
     1081 gcgccatgaa ccgcctcggt gaagtcgatc cgaacctcgg tctcgacgtc ggtaccgcgc
     1141 cgcgggcggc tggccccggc gttgccggca ccgcggttgg cgaacagccc gccgagcagg
     1201 tcgccgagac cgcccgcatc accaccacga ccggtggtgc tcccgaacag gtcaccgagg
     1261 tcgaacccac ccgcgccagc cgggccaccg aagccaccgc cgggccggaa acccccgttg
     1321 aacaagctgc gcgcctcgtc gtactgcttg cgcttctcgg ggtccgacaa cacaccgtag
     1381 gcctcggaca ccgccttgaa gcgcgcctca gcgcgactgt ccccgggatt ggcgtccgga
     1441 tgcagcttgc gggccagctt gcggtacgcc cgtttgatgt cgtcggcaga cgcggtggag
     1501 gagacaccca gctccgcgta gaaatccttt tcgatccact cccgtgcgct caccggacac
     1561 ctcctccctg agtcagttct cctgcaacgg ttcagcgccg ccggaccatg cggcgtcacg
     1621 cgacatagcc gcgtctgaag acatctccgc cggctcggta gtttccgacg tgtccccaac
     1681 cggctcggcg tcggttaccg ccaccatcgc cgggcgtagc acccgctcgc cgaggcggta
     1741 gccgcgacgc agcacggcgg tgacggtcgg ctcgtggacg tccggggagg tgtcgtgatg
     1801 caccgcctcg tgcacagctg gatcgaagcc gtccccgatg gccccgaaag cttccaggcc
     1861 gtgcgcctgc agcccggcca ccagcttgtc cgcgacggcc ttgaacgctc cggacaggtc
     1921 gccgtgctcc tgcgcccgct cgatgtcatc gagcacggtg agcaggtcac tggcaacctg
     1981 cgccttggcc aaggcaatcg tcagctggcg gtc
//
LOCUS       DATADN010000338         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_641363, whole genome shotgun sequence.
ACCESSION   DATADN010000338 DATADN010000000
VERSION     DATADN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641363"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /gene="efp"
                     /locus_tag="VHH53_04450"
     CDS             <1..493
                     /gene="efp"
                     /locus_tag="VHH53_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX2289444.1"
                     /translation="FQHVKPGKGGAFVRTTLKHVLSGKVVDKTFNAGTSVDTATVDRR
                     EMTYLYPDGADFVFMDTDTFEQITVPGETVGDAARYLLDNQTALVAVHDGTPLYVELP
                     TSVELVISHTDPGLQGDRSTGGTKPATLETGAEIQVPLFVTTGEKVKVDTRDGRYLGR
                     VSS"
     gene            495..899
                     /gene="nusB"
                     /locus_tag="VHH53_04455"
     CDS             495..899
                     /gene="nusB"
                     /locus_tag="VHH53_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX2289445.1"
                     /translation="MGARSKARKRAVDILFESDLRGLDPLALLAERVGAPDLPPVSDY
                     AVALVNGVVAHQQDIDRMLAEQARGWTVERMPAVDRAVLRLGVFELLFSDEVDDAVAI
                     DEAIELAKSLSTDDSPRFVNGVLGTLARQARS"
     gene            complement(933..1424)
                     /locus_tag="VHH53_04460"
     CDS             complement(933..1424)
                     /locus_tag="VHH53_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX2289446.1"
                     /translation="MGDYAKALGSKLRGIRQQQGLSLHGVEQKSAGRWKAVVVGSYER
                     GDRAVTVQKLAELADFYGVPVPELLPEGRIPSGGEPATKIVINLERLQQLPVDKVGPL
                     ARYAATIQSQRGDYNGKVLSIRTEDLRSLAIIYDMSPGDLTEQLIGWGVLPPEARPAK
                     EES"
     gene            1698..>2147
                     /gene="pyrR"
                     /locus_tag="VHH53_04465"
     CDS             1698..>2147
                     /gene="pyrR"
                     /locus_tag="VHH53_04465"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX2289447.1"
                     /translation="MSSPFGTGAAGPPRQRELLSAADVTRIVARMAHQIIERTAAQPV
                     ALLGIPTRGVPLAHRLADRIHEFGGSLAGVGILDITLYRDDLRRRPTRPLQPTELPAG
                     GLDDALVVLVDDVLCSGRTVAAALDAVRDYGRPRVIQLAVLVDRGHRE"
BASE COUNT          357 a          708 c          694 g          388 t
ORIGIN      
        1 tttccagcac gtgaagccgg gcaagggtgg cgccttcgtg cgcaccacgt tgaagcacgt
       61 cctgtccggc aaggtcgtgg acaagacctt caacgcgggc acctcggttg acaccgccac
      121 cgttgaccgg cgcgagatga cctacctgta ccccgacggt gccgacttcg tcttcatgga
      181 caccgacacc ttcgagcaga tcaccgttcc cggtgagacc gtcggagatg cggcgaggta
      241 cctgctggac aaccagacgg cgctggttgc cgtgcatgac ggcactccgc tgtatgtcga
      301 gctacccacc tcggtggagt tggtgatctc acataccgat ccgggcctgc agggagaccg
      361 gtcgactggc ggcaccaagc ccgccacgtt ggagaccggc gcagagattc aggtgccgct
      421 gttcgtcacc accggcgaga aagtcaaagt cgatacccgg gacggtcgct acctcggccg
      481 cgtcagctcc tgacgtggga gcccgcagca aggctcgtaa acgcgccgtc gacatcctct
      541 tcgaatccga cctgcgcggg ctggatccgt tggccttact cgctgagcgg gtcggggcac
      601 cagatctgcc tcccgtcagc gactacgccg tcgcattggt gaacggcgta gtagcccacc
      661 agcaagacat cgaccggatg ctcgccgagc aggcccgagg ttggaccgtt gagcggatgc
      721 ccgcggtgga ccgcgcggtg ctgcggctgg gcgtgttcga actgctgttc tccgacgagg
      781 tggacgatgc ggtggccatc gacgaggcga tcgagctggc caagtcgctg tccactgacg
      841 actcgccgcg attcgtcaac ggggtgcttg gcacgcttgc gcgccaagca cgctcatgag
      901 ctgggtttaa gtgctctggc acttaacgcc actcacgact cctccttggc ggggcgagct
      961 tccgggggta gcacccccca gccgatcagc tgctcggtga gatcacccgg cgacatgtcg
     1021 tagatgatcg ccagtgagcg cagatcctcg gtgcggatgg acagcacctt gccgttgtag
     1081 tcgccgcgct ggctctggat cgttgcggcg taccgagcca gtgggcctac cttgtccacc
     1141 ggcaactgtt gcagtcgctc caagttaatg acgatcttgg ttgctggttc tcctccggag
     1201 ggtatccggc cttctggtaa cagttccggc accggaaccc cgtagaagtc ggcaagctca
     1261 gcaagcttct gcacggtgac cgcgcggtct ccgcgctcat aggagccgac caccacggcc
     1321 ttccagcggc ccgccgactt ctgctcgaca ccgtgcagcg acagcccctg ctgctgacgg
     1381 atgccgcgca atttggaccc cagggccttg gcgtagtcgc ccatctggcg cttctccttc
     1441 ttcttcaacc ccgaccaggg cgccgcgcgc gcatcgggat gatacggaga gtaatgctgc
     1501 gctgagtaac ctcccaggtc aagccattgg gggcccaaca ctgaggagag tcacacacac
     1561 cacccggacg gggcagcgct tgagcgccga taatccggga tcaggcgcct gataccgtca
     1621 gcgttcgggt ccgggcgcct gcccggcacc cgatgtcctt taagacccgt ccggtgaggc
     1681 ggggaaggag ctcgaccgtg tcgtccccct tcgggacggg tgcggcagga ccaccgcggc
     1741 agcgtgagct gctctcggcg gccgatgtca cgcgcatcgt cgctcgaatg gcgcaccaga
     1801 tcattgagcg caccgccgcc cagcccgtgg cgttacttgg cattcctacc cgcggcgtcc
     1861 cgctggcgca ccggctggcc gaccggatcc atgagttcgg gggaagtctg gcaggcgtgg
     1921 gcatcctgga tatcacgctt tacagggatg acctgcggcg caggcctacc agaccgctac
     1981 agcccaccga gttgcctgct ggcgggctag acgatgccct ggttgtgttg gttgatgatg
     2041 tgctgtgctc cggccgcact gtcgcggcgg cgctggacgc ggtgcgcgat tacggccgcc
     2101 ctcgggtgat acaactcgcc gtgctggtcg accggggcca ccgtgaa
//
LOCUS       DATADN010000339         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_787966, whole genome shotgun sequence.
ACCESSION   DATADN010000339 DATADN010000000
VERSION     DATADN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787966"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1479
                     /locus_tag="VHH53_04470"
     CDS             202..1479
                     /locus_tag="VHH53_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX2289448.1"
                     /translation="MSATDRSSGRHHYRRATGPARPPPGHGHGHGGHGPVPPASRRVK
                     MLLAAALVPVALATLVGLWLTWPSDTPRSGVDIGFGQRPVHGEILAARSAPCSSASAG
                     IGEAEEAKKCISLDVQLTDGDSPGATIQQVLPDEPSTPRFTVGDQIVLAYTGGNPTDP
                     AAYQIQDFQRGVSMLWLVALFAAAVLLLGRWKGLASLAALGFSFLVLAVYLFPAILNC
                     ADPVIVGVLAAGVIMFGVLYLTHGVCAQTSSAVLGTVLSLGLIGVLGTAFSAATQLTG
                     LDDDTANLISVLGQGIDARGLLLAGMIIGALGVLNDVTVTQTSAVWELRRANPALSAR
                     ALYTAALRIGRDHISSAVNTLALAYAGAALPVLLAFSLSGQSFGTLITAQNVAQEVVR
                     TLVGSIGLVAAVPITTAIAAVVVTRDHIASTQH"
     gene            complement(1547..>1955)
                     /locus_tag="VHH53_04475"
     CDS             complement(1547..>1955)
                     /locus_tag="VHH53_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2289449.1"
                     /translation="SADARQFFGGRVQLREPREVRALEDGTSLELAGLSFTVDHTPGH
                     TRGSVTFRSVSGDRQRLLVSGDTLFAGSIGRTDLPGGDHEQMLTSLQNKILVLDDETV
                     VLPGHGPQTTIGRERATNPFLHGLAAPGAARGL"
BASE COUNT          308 a          657 c          645 g          345 t
ORIGIN      
        1 cgcgtatctc atagaaggtc atcccgcgga actccggggt gtcgaaggtg cgcgtcatga
       61 cgtcgcccac tccgaacagc gcctcctggg cggactcatc ggacagcgtc aagttgtccc
      121 atcgcatcgg cccaccttat cgaactgatg ttcgatccgc taggaactgg ggtgcaccga
      181 tcagagaggg atacccagtc gatgagtgct actgaccgca gcagcggcag gcaccactac
      241 cgccgggcta ctggaccggc acgcccgcca ccaggccatg gccacggcca tgggggacac
      301 ggaccggtgc cgccggcctc tcgccgggta aagatgctgc tggccgcggc gttggtgccg
      361 gtcgcgctgg ccaccctggt tggcctgtgg ctgacctggc ccagcgacac cccacgcagc
      421 ggtgtggaca tcgggttcgg gcagcggccg gtgcacgggg agatcctggc ggcgcgctcc
      481 gccccatgca gcagcgccag cgcgggcatc ggcgaggccg aagaagccaa gaagtgcatc
      541 tcgctggatg tccagctcac cgacggcgac tcgccgggtg ccacgatcca gcaggtgtta
      601 cccgacgaac ccagcacgcc gcggttcacc gtcggtgacc agatcgtgct cgcctacacc
      661 ggcggaaatc ccaccgatcc tgccgcttat cagatccagg atttccagcg tggtgtgtcg
      721 atgctgtggc ttgttgcgct gttcgccgcg gcggtactgc tgcttggcag gtggaaaggc
      781 cttgcgtcgc tcgccgcgct cggcttcagc tttctggtac tggcggttta cctgttcccc
      841 gcgatcctca actgtgcgga ccccgtgatt gtcggcgtgc tggccgccgg tgtgatcatg
      901 tttggggtgc tgtatctcac ccacggtgtc tgcgcccaga catccagcgc agtgctgggc
      961 acggtattga gcctcggcct aatcggcgtg ctgggtaccg ctttctccgc agccacccag
     1021 ctgaccggat tggacgacga caccgccaac ctcatcagcg tgctcgggca gggaatcgac
     1081 gcccgcgggc tgctgctcgc cggaatgatc atcggtgcac tgggcgtgct caacgatgtc
     1141 acggtgaccc agaccagcgc ggtgtgggaa ctacgtcgag ccaatcccgc cctgagcgcc
     1201 cgcgccctct acaccgctgc attgcggatc ggacgtgacc acatttcctc ggcggtgaac
     1261 accctcgccc tggcctacgc cggtgccgcg ttgcccgtgc tgctggcttt ctcgctgtcc
     1321 gggcagagct tcggcacgct gatcaccgca cagaacgtcg cccaggaggt ggtgcgcacc
     1381 ctggtgggca gcatcgggct cgtcgctgcc gtcccgatca ccacagcaat cgccgcggtg
     1441 gtcgtcaccc gagaccacat cgcatcaacg cagcactaga ccgtgagttg gtagtcgttt
     1501 gtgacgccgt gagggcgggt tggtagccct tgaagggcac ccgagctcac aggccccgcg
     1561 ctgcgccggg ggcggcgagg ccgtgcaaga acgggttggt ggcacgttct ctgccgatgg
     1621 tggtctgcgg gccgtggccg ggcagcacga cggtctcgtc gtcgagcacg aggatcttgt
     1681 tttgaagcga cgtgagcatc tgctcgtggt cgccaccggg taggtcggtt cgtccgattg
     1741 agccggcgaa cagggtgtct ccggagacca gcagacgctg cctatcgcca ctgaccgatc
     1801 ggaacgtcac cgatcctcgg gtgtgccccg gggtgtggtc gacggtgaag ctcagcccgg
     1861 ccagctccaa ggacgtaccg tcctccagcg cgcgcacctc gcgcggctcg cgcagctgga
     1921 cccgaccacc gaagaactgc cgcgcatcgg cggac
//
LOCUS       DATADN010000340         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_183595, whole genome shotgun sequence.
ACCESSION   DATADN010000340 DATADN010000000
VERSION     DATADN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183595"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VHH53_04480"
     CDS             complement(<1..357)
                     /locus_tag="VHH53_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX2289450.1"
                     /translation="MGCDSEVGRLRAVILHRPGAELQRLTPRNNDALLFDGLPWVARA
                     QQEHDAFAVLLADRGVEVLLLADLLTEALSHSGAARMHGIAAAVDPRRLGLPLAQELS
                     AYLRSLEPAPLARVLMA"
     gene            451..852
                     /gene="soxR"
                     /locus_tag="VHH53_04485"
     CDS             451..852
                     /gene="soxR"
                     /locus_tag="VHH53_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEX2289451.1"
                     /translation="MSARSGVAVSALHFYEREGLITSRRTGGNQRRYARETLRRVAFI
                     RMSQRLGIPLARIREALATLPTDRVPTSKDWARLSAGWRQDLDDRILHLQRLRDNLAG
                     CIGCGCLSLKTCSLANPGDVLAGSGPGPVRL"
     gene            886..1482
                     /locus_tag="VHH53_04490"
     CDS             886..1482
                     /locus_tag="VHH53_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX2289452.1"
                     /translation="MEQALQGSLFEHAERRILGNGAWLDVRSGWLTDADTLFDELRDG
                     IPWRAERRQMYDRVLDVPRLLSFHNLVEDEAPHPRLKQMRRRLNDTYGGELGEPFTTA
                     GLCLYRDGNDSVAWHGDNIGRSSSEDTMVAIVGLGATRVFALRPRGGGPSLRIQHHHG
                     DLLVMGGSCQRTWEHAIPKTTRPTGPRISIQFRPHDVR"
BASE COUNT          265 a          562 c          479 g          208 t
ORIGIN      
        1 tgccatcagc acccgggcta gcggtgccgg ctccagcgac cgcaggtacg ccgaaagttc
       61 ttgcgccagc ggcaaaccca accggcgcgg atcgaccgcc gctgcgatac cgtgcattct
      121 ggccgcgccg ctatgcgaca gcgcctcggt cagcaggtcc gccagcagca gcacctcgac
      181 cccgcggtcg gccaacagca cggcgaacgc gtcgtgctcc tgctgcgccc gggccaccca
      241 aggcagcccg tcgaacaaca gggcatcgtt gttgcggggc gtcaaccgct gcagttcggc
      301 gccgggtcgg tgcaggatga cggcacgcag cctgccgacc tcggagtcgc aacccagcgt
      361 cacacctagc accgtaacgc cacgggccct cacgaccacc tcaactatgg ttaaggtcat
      421 ggaactcgtc cacgaactca ccccggcgaa atgtcggccc gcagtggtgt ggcggtttcg
      481 gcgctgcact tctacgagcg cgaaggtctg atcaccagca ggcgcaccgg cggcaaccaa
      541 cgccgctacg cgcgcgagac gctgcggcgg gtcgcgttca tccggatgtc gcagcggctg
      601 ggcataccgc tcgcacggat ccgcgaagcg ctggccaccc tgcccaccga ccgcgttccg
      661 accagcaagg actgggccag gctctccgcg ggctggcgtc aggacctcga cgaccgcatc
      721 ctgcacctgc agcggctgcg cgacaatctc gccggctgca tcggctgcgg ctgcctgagc
      781 ctcaagacct gcagccttgc caaccccggc gacgttctcg ccggaagcgg accgggaccc
      841 gttcgcctct gagaagtcga acgcctgtgc gatacactcg gatccatgga gcaggctcta
      901 cagggctcgc ttttcgagca cgcagagcgc cgcatactcg gcaacggcgc atggctcgac
      961 gtccggtcgg gctggctgac cgacgccgac accctgttcg acgaactgcg tgacggcatc
     1021 ccctggcggg ccgagcgccg acagatgtac gaccgggtgc tcgacgtgcc acggctgctg
     1081 agcttccaca acctcgtcga agacgaggcg ccccatccgc gcctcaaaca gatgcgccgg
     1141 cggctcaacg acacctacgg cggcgaactc ggtgagccct tcacgaccgc cgggctctgc
     1201 ctctaccgcg acggtaacga cagcgtcgcg tggcatggcg acaacatcgg tcgcagcagc
     1261 agcgaggaca ccatggtcgc gatcgtcggc ctcggcgcca cccgggtctt cgcactgagg
     1321 ccgcgcggcg gcggcccgtc gctgcgcatc cagcatcacc acggcgatct gctcgtgatg
     1381 ggcggatcct gccagcgcac atgggaacac gcgatcccga agaccacccg accgaccggc
     1441 ccgcggatca gcatccagtt ccgaccgcac gacgtgcggt gaccggtcag ccctgaacgg
     1501 cggcgaccgc gtcg
//
LOCUS       DATADN010000341         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_495968, whole genome shotgun sequence.
ACCESSION   DATADN010000341 DATADN010000000
VERSION     DATADN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495968"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VHH53_04495"
     CDS             complement(<1..1444)
                     /locus_tag="VHH53_04495"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HEX2289453.1"
                     /translation="MSVTPNRTPDSNGWYNRAVGFSASGSNYGPSGAGSCDADTSYDG
                     PDSASASVLMSCTDGAGNSGSGSARFMYDDTAPSVTVSLARGADHNGWHNHAVGYGVS
                     ARSDATSGIASCDASDTYSGPDDATASVSRTCTDVAGNVGSDTALFKYDATAPADVAT
                     TLNRVADHNGWYNAPVGWTTNGDDLTSGIDTCDSDTYNGPDGTGLTVSGRCVDKAGNP
                     SASVSAAFKYDNTNPNVSGTLARAADHNGWYNTSVSYSFASSSTDDTSGIDSASCTSG
                     TYNGPDGSSVTVTGTCSDNAGNSANGSTPPFKYDNTDPTLAPTISPNPVLLNGSATAS
                     ANANDATSGVDSSSCDPVNTGTIGANQSVSCSATDKAGNSNTANATYSVKADFNGFLQ
                     PIDGHSVNTGKYGRTYPIKWQLRDSSGALISDSAAQLLVGTMTGGQKAVSCTSFDLTD
                     TDALEASTTGNTALRYDATSDQFIYNYKAPS"
BASE COUNT          241 a          446 c          535 g          321 t
ORIGIN      
        1 aagacggcgc cttgtagttg tagatgaact ggtcgctggt cgcgtcgtag cgcagagccg
       61 tattaccggt ggtggacgct tcgagcgcat cggtgtcggt aaggtcgaag ctcgtgcagg
      121 aaaccgcctt ctggccaccc gtcatcgtgc caaccaggag ttgggccgca ctgtcgctga
      181 tcagggcgcc ggagctatcc ctgagctgcc actttatggg gtaggtcctg ccgtacttac
      241 cagtgttgac ggagtgcccg tcgatcggct gcaggaaccc gttgaagtct gccttgacgc
      301 tgtacgtggc gttggcggtg ttggagttac cagccttgtc cgtcgcggag cagctgacgc
      361 tctggttggc gccgatggtg cccgtgttga cggggtcaca gctggaggag tcgacgccgc
      421 tcgtggcgtc attagcgttc gcgctggccg tcgcgctccc gttgagcagg acagggttag
      481 gcgagatggt cggggccagt gtggggtcgg tgttgtcgta cttgaagggg ggggtagagc
      541 cattggcgct gttacccgcg ttgtctgagc acgtaccggt gaccgtcaca ctgctgccgt
      601 cggggccgtt gtaggtgccg ctggtgcagc tagccgaatc gatgccgctg gtgtcgtcag
      661 tgctgctgct ggcgaacgag tagctaacag aagtgttgta ccagccattg tggtcagccg
      721 cgcgagccag ggtgcccgag acgttcgggt tggtgttgtc gtacttgaac gcggccgata
      781 cggaggccga tgggtttcct gccttgtcga cgcagcgtcc gctcacagtc aggccagtgc
      841 catcgggccc gttataggtg tcactgtcac acgtgtcgat gccgctggtc aaatcatcac
      901 cattggtcgt ccaaccgacc ggcgcgttgt accagccgtt gtgatcagcg acacggttga
      961 gcgttgttgc aacgtcggcc ggcgcggttg cgtcgtactt gaacagcgcg gtgtcggagc
     1021 caacgttgcc agccacgtcc gtgcacgtcc ggctcacgga cgcggtcgca tcatccgggc
     1081 cgctataggt gtcgctggcg tcgcagcttg cgatcccgga ggtcgcatcg ctgcgggcag
     1141 agacaccgta gccgaccgcg tggttatgcc agccattgtg atcggcaccc cgcgcgagcg
     1201 agacggtcac ggacggtgcc gtatcgtcgt acatgaagcg ggccgaaccc gagccggagt
     1261 ttccggcacc gtcggtgcag ctcatcaaga cggacgccga tgcgctgtcc ggaccgtcgt
     1321 agctcgtgtc cgcatcgcag ctgccagcac cgctcggtcc gtagttcgag ccagaggccg
     1381 agaagccgac ggcgcggttg taccagccat tgctgtcagg agtacgatta ggtgtgacgc
     1441 tcacgtcggg ggcttccttg tcgatcttca gcgtgactga gtcgctcgcc gatccgcctg
     1501 cgctatgcgc cgaacaggaa gacgttgcgg acgcggtgct cga
//
LOCUS       DATADN010000342         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_950768, whole genome shotgun sequence.
ACCESSION   DATADN010000342 DATADN010000000
VERSION     DATADN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950768"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VHH53_04500"
     CDS             <1..386
                     /locus_tag="VHH53_04500"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX2289454.1"
                     /translation="PVALLRTAGGADIAAMAAFLAQAAVRRTPTILDGVAVGAAAMLA
                     EELAPGARQWWVAGHRSVEPAHTLVLEHLELDPILDLGMRLGEGSGAVAALPLLQLAV
                     QILAEMATFDVAGVSGPTGDPVPTR"
     gene            383..1132
                     /gene="cobS"
                     /locus_tag="VHH53_04505"
     CDS             383..1132
                     /gene="cobS"
                     /locus_tag="VHH53_04505"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX2289455.1"
                     /translation="MSRGLRLAVSWLTVLPVGAPVEVDRAVARRALYWAPVVGAGLGA
                     TAVGVLAALSALDAPPLLTGLAVVAALACLTRGMHLDGLADTADGLGCYGGPQRALAV
                     MRDGSTGPFGVVALVLVLITQAAALGALTETGRLAAVLLAVAAGRVAFIWCARTGLPP
                     ARREGLGALVAGSQPPAVAVAWSATLLAGGLMVVPGRAWQGALAVALAALAILAFSAH
                     TRRRFGGVTGDVLGAACELTTTVVLAVCALG"
     gene            complement(1212..2315)
                     /locus_tag="VHH53_04510"
     CDS             complement(1212..2315)
                     /locus_tag="VHH53_04510"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX2289456.1"
                     /translation="MSAAPPFTRLPHPSPTPPQRRAQILATPGFGRYFTDHMVDISWT
                     DGVGWHDPQIGPYRQLVMDPAAMVLHYGQAIFEGLKAYRQPDGTVSSFRPLANAARLR
                     SSARRMAMPELPDELFVGSLRELVRTDIDWVPAAGGEECLYLRPFLFATEAGLGVRPA
                     ATYRYLLIASPAGPYFRGGLEPVSVWLSTEYVRAAPGGTGAAKSAGNYAASLVAQREA
                     ADKNCDQVVWLDAVERRWVEEMGTNNLYFVFGRGQQARLVTPELTGTLLPGITRDSLL
                     TLARDHGMGVEERRISTEEWEKGVETGDISEVFACGTAAVITPVGRVAYPGGEFTIGS
                     GEPGPVTARLRAALTAIQRGAAPDTHNWMTPLT"
     gene            2374..>2734
                     /locus_tag="VHH53_04515"
     CDS             2374..>2734
                     /locus_tag="VHH53_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289457.1"
                     /translation="MRHADQVPEQRSRVRITRIRLADGRALTYYDDREPYLSGTATRA
                     VADLRDLSPYQAQPMMRLDVLTGEWVIIAPHRMDRTHLPPAGECPLCPSRDGKMTEIP
                     GEDYDVVVFDNRFPCLGP"
BASE COUNT          366 a          936 c          999 g          433 t
ORIGIN      
        1 accctgtcgc gctcctgcgt accgcaggcg gcgccgacat cgcagcgatg gctgccttcc
       61 tggcccaggc cgcggtccgg cgcaccccga ctatcctcga cggcgtggcc gtcggggccg
      121 cggcgatgct cgccgaggaa cttgcccccg gagcacggca gtggtgggtg gctgggcacc
      181 gctccgtgga gcccgcgcat accctggtgc tggagcacct ggaactggac cccatccttg
      241 atctgggaat gcggctgggt gagggctccg gggcggtggc cgcgctgccc ctgcttcagc
      301 tggccgtgca gatactcgcc gagatggcca cctttgacgt cgccggcgtc tctgggccca
      361 caggtgatcc cgtgccgacg aggtgagccg cgggttgcgg ctggcggtgt cctggctgac
      421 cgtgctaccg gtcggcgcgc ccgtcgaggt ggaccgggca gtcgctcgcc gggcgctgta
      481 ctgggcgccc gtggtcggcg ccgggctggg cgcaactgca gtcggggtac ttgccgcgct
      541 gtccgcgctg gacgctccgc cgctgctgac cgggctcgcc gtggtggcgg cgctggcttg
      601 cctgacccgc gggatgcacc tggacgggct cgccgacact gccgacgggc tgggctgtta
      661 cggggggccg cagcgggccc tggcggtgat gcgcgacggg tcgaccgggc cgttcggagt
      721 ggtcgcgctg gtgctggtgt tgattacgca ggcggccgcg ctgggtgcgc tgaccgagac
      781 gggccggctg gccgcggtgc tgcttgcggt ggccgctggg cgggtcgcct tcatctggtg
      841 cgctcgcacg gggttaccgc cggcacggcg agaggggttg ggcgcgctgg ttgccggtag
      901 ccaaccgccc gctgttgcgg tggcctggtc agcaacgttg ctggccggtg gactgatggt
      961 ggtgcccgga cgggcttggc agggcgcgct ggcggtggcc ctggcggccc tggcgatcct
     1021 ggcgttctcg gcgcacaccc ggcgccgctt cggcggtgtc acaggtgacg tactaggtgc
     1081 cgcctgcgag ctgaccacca ccgtagtact cgccgtatgc gcgctcggct gaccaggctt
     1141 agcgcattca gcgggctcaa cgggcttatc gggcaccgat ttaccccgct gagcccgctg
     1201 aatgcggggg gtcaggtgag gggcgtcatc cagttgtggg tgtcgggagc ggcgccgcgt
     1261 tggatcgcgg tgagggcggc gcgcaagcgg gcggtgacgg ggccgggctc gccgctgccg
     1321 atggtgaact caccgccggg gtatgcgacc cgccccacag gagtgatcac cgcggcggtg
     1381 ccgcaggcga agacctccga gatgtccccg gtctccacgc ccttctccca ctcctcggtg
     1441 gagatccgcc gctcctcaac tcccatcccg tggtcgcggg ccagcgtgag caaggagtcg
     1501 cgggtgatgc cgggcagcag tgtgccggtg agttccggag tcaccagtcg agcctgctgt
     1561 ccccttccga agacgaagta gaggttgttt gttcccatct cctccaccca gcgccgctcg
     1621 accgcgtcca gccacacaac ctggtcgcag ttcttgtccg cggcctcccg ctgggccacc
     1681 agcgacgcgg catagttccc ggcggacttg gcagctccgg taccgccggg cgcggcccgg
     1741 acgtattcgg tggacagcca cacgctgacc ggctcgagcc cgccgcggaa gtacgggcct
     1801 gccggggacg cgataagtag gtaccggtaa gtcgccgcgg ggcgtacccc gagccccgcc
     1861 tcggtggcga acaggaacgg tcgcaggtac aagcactcct ccccgccggc ggcgggcacc
     1921 cagtcgatat ccgtgcgcac cagttcgcgc agcgagccga cgaatagctc gtcgggcagc
     1981 tccggcatcg ccatccggcg ggccgaggag cgaagccggg cggcgttggc cagtggccgg
     2041 aaggaggaca ccgtgccgtc aggttgccgg taggccttga gaccttcgaa gatcgcctgc
     2101 ccgtagtgca gcaccatcgc ggccggatcc atgacgagct gccggtacgg accgatctgc
     2161 ggatcatgcc agccgacacc gtcggtccag ctgatgtcca ccatgtggtc ggtgaaataa
     2221 cgaccgaagc ccggcgtggc caggatctgc gccctccgct gcggcggcgt cggagacggg
     2281 tgcggcaggc gggtgaacgg gggcgcggca ctcatgtcca gcacggtatc ccgtcgcgcg
     2341 ccaaaatggt aggacgtccc actgtctgac tccatgcgcc atgctgacca ggtgcccgag
     2401 cagcgaagcc gcgtgcggat cacccgtatc cggctggccg acggtcgcgc gctgacctac
     2461 tacgacgacc gcgagcccta cctctcaggc acggcaaccc gcgccgtggc cgacctccgc
     2521 gacctgtccc cgtaccaggc tcagcccatg atgcgcctgg acgtgctcac cggagagtgg
     2581 gtcatcatcg ccccgcaccg catggaccgg actcacctgc ctccggcggg agaatgcccg
     2641 ctgtgcccca gccgggacgg caagatgacc gagatcccgg gtgaggacta cgacgtggtg
     2701 gtgttcgaca accgatttcc ctgcctgggg cccc
//
LOCUS       DATADN010000343         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_348109, whole genome shotgun sequence.
ACCESSION   DATADN010000343 DATADN010000000
VERSION     DATADN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348109"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VHH53_04520"
     CDS             complement(<1..334)
                     /locus_tag="VHH53_04520"
                     /inference="COORDINATES: protein motif:HMM:NF028506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5134 domain-containing protein"
                     /protein_id="HEX2289458.1"
                     /translation="MEAAAGQLWVSGVCAALFAGLTLFFAVRLLAFHPDTSGAGCSTD
                     RGADASHAVMSLGMAAMFWPWGHPVPALYWQVLFSVIAAWFAVQLLRRAAQPADLHHV
                     LGSLAMVYM"
     gene            complement(498..>1980)
                     /locus_tag="VHH53_04525"
     CDS             complement(498..>1980)
                     /locus_tag="VHH53_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEX2289459.1"
                     /translation="LVNNLGWLFVLAATGFVVFALWAALSRYGRIPLGRDGEQPEFRT
                     VSWVAMMFSAGMGIGLMFYGVSEPLTHFVEPPPGAEPGIGTAMATTLFHWTLHPWAMY
                     AVMGLALAYGTYRRGRPQLISAAFWPLLGKATAGPAGRAIDIMAIFATLFGSAASLGL
                     GALQIGAGFEANGWLYSPGNALLVTAIVVLTVAFIVSAVSGVAKGIQWLSNTNMVLAV
                     VLAVFVFVVGPTVLILNVVPTAVGDYFRDLASMSARTAAAGGDATNEWLSSWTIFYWA
                     WWISWTPFVGMFIARISRGRTIRQFVAGVIAVPSVVSLVWFAIFGGAAISAQRGGTDI
                     AGQGGVEAQLFGLLNTFPLAGVASVLVMVLVAIFFVSGADAASMVLGTLSQRGNQDPQ
                     RWNVIFWGAATGAVAIVMLLVGGATALSGLQTLTIIAAAPFALLMIALCVSLARDLRT
                     DPLIVADHAIERHLREQHHNRRNGSAETRKAVPSAVDRPTSGE"
BASE COUNT          395 a          711 c          613 g          261 t
ORIGIN      
        1 gcatgtacac catcgccagg ctgcccaaca cgtgatgcag atcagcgggc tgcgctgctc
       61 gccggagcag ctgaaccgcg aaccacgcag cgatgacgct gaacagcacc tgccagtaca
      121 gcgcaggtac cggatgaccc cagggccaga acatcgcggc catgccgagg ctcatcactg
      181 cgtgcgacgc atccgccccg cggtcggtgc tacagccagc tcccgaggtg tccgggtgaa
      241 aggcgagtag ccgcaccgcg aagaagaggg tcaacccagc gaacagcgcg gcgcacaccc
      301 cactcaccca cagctgtcct gccgccgcct ccacatccgc ctctttcccg catcacgccg
      361 gaccgcaccg ccttccacag ggatagtcgg ttgcacttcg gcgttggttc ccggtgttag
      421 acaccgctga ccgagatgcc gggactgcgt gcaggcgctg cacgacctgg cggacgccta
      481 tgacgagcgg ctgctggtca ctcgccacta gtgggccggt ccaccgcgga gggcaccgcc
      541 ttgcgtgtct ccgcggagcc atttctgcgg ttgtggtgct gctcgcgcaa gtgccgctcg
      601 atcgcgtgat ccgcgacgat caaggggtcg gtccgcagat cgcgggccaa cgatacacac
      661 agggcgatca tgagaagggc gaacggagcc gccgcgatga tcgtgagcgt ctgcaggcca
      721 gacaacgcgg ttgccccgcc gaccagcagc atgactatcg ccaccgcgcc ggtcgcggcg
      781 ccccagaaga tcacattcca gcgctgggga tcctggttgc cgcgctgcga caacgtgccc
      841 agcaccatcg acgcagcatc cgcaccggat acgaagaaga tcgcgaccag caccatcacc
      901 agcacgctgg ccaccccggc aagcgggaag gtgttcagca gcccgaacag ctgcgcctca
      961 acaccaccct ggccggcgat atcggtgcca ccgcgctgcg cgctgatcgc agcccccccg
     1021 aagatggcga accacaccag gctcaccacg ctgggcaccg cgatcacccc ggcgacgaac
     1081 tggcggatcg tccgtccccg gctgatccgg gcgatgaaca tgcccacaaa cggggtccag
     1141 gaaatccacc acgcccagta gaagatcgtc caactggaca gccactcgtt ggtcgcgtct
     1201 ccacccgcag cggccgtccg ggctgacatg ctggccaaat cgcggaagta gtcgccgacc
     1261 gcggtcggga ccacgttgag gatcagcacc gtcgggccga ccacgaagac gaacaccgca
     1321 agcaccactg cgagcaccat gttggtgttg gacagccact ggatgccctt ggccaccccg
     1381 gacaccgcgg agacgatgaa cgccaccgtg agcacaacga tggccgtcac cagcagtgca
     1441 ttacccgggc tgtacagcca gccgttggcc tcgaacccag cgccgatctg cagagcgccg
     1501 agtcccagcg aggccgccga gccgaacagc gtggcgaaga tcgccatgat gtcgatggcc
     1561 ctgcccgcag ggcctgctgt ggccttcccg agcagtggcc agaacgcggc gctgatcagc
     1621 tgcggccggc cgcgccggta agtcccgtaa gccagggcga gccccatcac ggcatacatc
     1681 gcccaaggat gaagggtcca gtggaacagc gtggtggcca tcgccgtgcc gatgcccggc
     1741 tcggctcctg gcggtggctc gacgaagtgc gtcaggggct cgctgacccc gtagaacatc
     1801 agaccgatgc ccatgcccgc actgaacatc atcgcgaccc aggacaccgt gcggaactcg
     1861 ggctgctcgc cgtcgcggcc cagcgggatc ctgccgtagc gagacaacgc cgcccacagc
     1921 gcgaacacca cgaagccggt ggcagcaagc acgaacagcc agccgaggtt attcaccagc
//
LOCUS       DATADN010000344         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_496813, whole genome shotgun sequence.
ACCESSION   DATADN010000344 DATADN010000000
VERSION     DATADN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496813"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..139)
                     /locus_tag="VHH53_04530"
     tRNA            complement(68..139)
                     /locus_tag="VHH53_04530"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Val,seq:tac)
     gene            complement(162..620)
                     /locus_tag="VHH53_04535"
     CDS             complement(162..620)
                     /locus_tag="VHH53_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX2289460.1"
                     /translation="MSIEVGDRAPDFTLKDQNNQEWTLSGFHSARNVLVVFYPFAFSR
                     ICTGELDQLRDDLAEFGDVQVLAVSVDSVFTLKAWSDQQGYDFPLLSDFWPHGKVAQD
                     YGVFNDKAGMANRGTFLVDTDGVVRFAELNQPGEARDLTTWKHALTALPA"
     gene            complement(701..1138)
                     /locus_tag="VHH53_04540"
     CDS             complement(701..1138)
                     /locus_tag="VHH53_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEX2289461.1"
                     /translation="MVAAAAGDAGKLGVAERLGIQSDMVVQELGWDIDVDDDVRAAIE
                     EVLGAELLDEDSDEIVDVVLLWWREEDGDLVDTLVDARSPLADNGVIWVFTPKTGRDG
                     HVEPSDINEAANTVGLAQTSNISVSDDWAATRLATAKAAKSKR"
     gene            1414..>1603
                     /locus_tag="VHH53_04545"
     CDS             1414..>1603
                     /locus_tag="VHH53_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289462.1"
                     /translation="MTTQQGRLLARVRVIRDGVAAHLPDIDPEETAEWLEAFDSAVLS
                     GGKQRARSSSRSIRYRARC"
BASE COUNT          271 a          540 c          514 g          278 t
ORIGIN      
        1 cgacctggcg tcggcctgct gccgtcgatc atgacaggag tcgggtgtcg gtgctggtgg
       61 tgccgggtgg gcgcggcagg tttcgaacct gcgacccctc gcttgtaagg cgagtgctct
      121 cccgctgagc tatgcgcccg atgccccgtg gagcacggtc ttcacgctgg tagtgcagtc
      181 agggcgtgct tccaggtggt caggtcccgg gcctcgccgg gctggttgag ctcggcgaag
      241 cggaccacac cgtcagtgtc caccaggaag gtaccgcggt tggccatccc agccttgtcg
      301 ttgaataccc cgtagtcctg cgcgaccttg ccgtgcggcc agaagtcgga cagtagtggg
      361 aaatcatagc cctgctggtc agaccatgcc ttgagggtga acaccgagtc cactgacacc
      421 gccagcacct gcacgtcgcc gaactccgcc aggtcgtcgc ggagctggtc gagctcgccg
      481 gtgcagatcc ggctgaaggc gaacgggtag aacaccacca gcacgttgcg ggcactgtgg
      541 aaccccgaca gcgtccactc ctggttgttc tgatccttca gggtgaagtc cggggcccgg
      601 tccccgacct cgatcgacat gtgcgcagct cccctcgacc cgttcgcagc ccgcgctgga
      661 tggccgcgcg ctgacgccga gagtatccac aaccgagaag tcaccgtttg gacttggccg
      721 ccttcgccgt ggctagcctg gtggctgccc agtcgtcgct cacgctgatg ttggacgtct
      781 gcgccagccc gactgtgttg gcggcctcgt tgatgtccga gggctcgaca tgaccgtcgc
      841 ggccagtctt aggcgtgaac acccagatca caccgttatc ggccaacggc gaccgcgcgt
      901 cgaccaaggt gtccaccaga tcaccatctt cctcgcgcca ccacagcagc accacatcaa
      961 cgatttcgtc ggagtcttcg tcgagcagct cggcaccaag cacctcctcg atggccgcgc
     1021 gcacgtcatc atcgacgtcg atatcccagc cgagctcctg tacaaccatg tccgattgga
     1081 tcccgagcct ctcggcaacg ccgagcttgc cggcgtctcc cgccgcggcg gcgaccacgg
     1141 tttcacccct ccacagcatc aggccggcgt cgaccgccgg acgtgttact actcgatgac
     1201 gtaccagcga acactgcggt cccgccagag cgcaacagcc agtaccagga cacgccggtg
     1261 ctggcgtagg tcgcacgaat taccagcggg taagagtgac accgcgggcg aggcggacga
     1321 gcatggagca cgatggaggc aggtggccgg tcccgcgcga ccggcaggct ggtcacttgc
     1381 ggttttccag acacctcgag gcgaggagat cccttgacca cgcagcaagg ccgcctgctc
     1441 gctcgtgtgc gggtaatccg tgacggggtg gcggcgcacc ttccagatat cgacccggag
     1501 gagacggcgg agtggctgga ggccttcgat tccgcggtgc tgtccggcgg caagcagcgg
     1561 gcccgttcga gcagccgcag catcaggtat cgtgcccgct gct
//
LOCUS       DATADN010000345         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_951682, whole genome shotgun sequence.
ACCESSION   DATADN010000345 DATADN010000000
VERSION     DATADN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951682"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1560
                     /locus_tag="VHH53_04550"
     CDS             82..1560
                     /locus_tag="VHH53_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX2289463.1"
                     /translation="MRNGYLDGNYAPVQQEHTITELAVTGPIPEHLDGRYLRNGPNPV
                     AEIDPQTYHWFMGDGMVHGVRLRDGKAEWYRNRWIRSPAVAKALGEPARHRRHRAGLE
                     FLAANTNVLSHAGRTLALVEGGVASYELTDELNTVGPCDFDGSLRGGYTAHPKRDPDT
                     GELHAISYFFGRGNTVQYSIIGVDGRARHTVDIPVTGSPMMHDFSLTQNHVVIYDLPV
                     TFDPVQGAEIIFPRALRTPVRLVLSALIGRVRVPDPITAMFGKCARGNANVPYSWDPT
                     YPAQIGVLPRHGGAHDIRWFDVAPCYVFHPLNAYEEAGTIVLDVVRHPKIFDSVDGPG
                     EGRPTLDRWTVDLAAGNVCETRLDDRPQEFPRIDERLLGRPHRYGYAMQAASGAAAIA
                     VDAVLKHDLHRGRTTVRRLGRHKQPGEFVFIPNTPDAAQDDGVLMSFVNDASTNRSDL
                     MLLDAATLETIAAIHLPERVPHGFHGNWSPSCLSEANQEAHQ"
     gene            complement(1554..>2106)
                     /locus_tag="VHH53_04555"
     CDS             complement(1554..>2106)
                     /locus_tag="VHH53_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2289464.1"
                     /translation="KVTKGALYFHFASKQALADAVVAEMVARWRVMVAEVNARGLDPL
                     WSLLAQTDEVVVRKIEDPIARGGIQLLTDPGVVCADAAAHYVYGEAAAKEQLTAAAAG
                     GLLRPGVDPPQIARVVVSMITGANLLCDLFRGHNALWEAVTELWQGLLPAIATDPWLE
                     SWRAADWPRRPMPVISGLPGLPH"
BASE COUNT          395 a          744 c          648 g          319 t
ORIGIN      
        1 accgcggcga ctcgaaaacc ctacaatccg gacagtagaa cggggtgatc gggcggtctt
       61 gtgagggagc agactgcggc gatgcgtaac ggctacctgg atgggaacta cgctccggta
      121 cagcaggaac acaccatcac cgagcttgcg gtgacgggcc ccatcccgga gcatctcgac
      181 gggcggtacc tgcgcaacgg gcccaacccg gtcgccgaga tcgacccgca gacctaccac
      241 tggttcatgg gcgacgggat ggtgcacggg gtgcggctgc gagacggaaa ggccgagtgg
      301 taccggaacc gctggattcg aagccccgcc gtcgccaagg cgctcggcga accggcgcgc
      361 caccgccgcc accgtgccgg gctggagttc ctggcggcga acaccaacgt gctcagccac
      421 gccggccgca ccctggcact ggtcgaaggc ggggtcgcca gctacgagct caccgacgaa
      481 ctgaacaccg tcggaccctg tgacttcgat ggctcgctgc gcggcggcta caccgctcac
      541 cccaaacgcg accccgacac cggagagctg cacgcgatct cctatttctt cggccgcggc
      601 aacaccgtgc agtactcgat catcggagtc gacgggcgcg ctcggcatac cgtcgatatc
      661 ccggtcacgg gcagcccgat gatgcacgac ttctcgctca cccaaaacca cgtagtgatc
      721 tatgacttac cagtcacttt tgatccggtt caaggcgccg aaatcatatt cccacgcgca
      781 ttgcgaacac cggtacggct ggtgctctcc gcgctcatcg gtcgcgtccg ggttcctgat
      841 ccgatcacgg ccatgttcgg gaaatgcgcc cgcgggaacg cgaacgtgcc ctactcgtgg
      901 gatcccacct acccggctca gatcggagtt ctgccgcggc acggtggtgc tcacgacatc
      961 cggtggttcg acgtagcgcc ctgctatgtc tttcacccgc tcaacgcata cgaggaggcc
     1021 ggcacgatcg tcttggacgt ggtgcggcat cccaagatct tcgacagcgt cgacggaccc
     1081 ggcgagggcc ggcccacgct ggaccgctgg accgtcgatc tcgcagccgg caacgtgtgc
     1141 gagacccggc tcgatgaccg ccctcaggaa ttcccgcgta tcgacgagcg actcctgggc
     1201 cggccacacc gttacggcta cgccatgcag gcagcgtcgg gcgccgcagc catcgccgtc
     1261 gacgccgttc tcaaacatga tctacatcgg ggtcgcacca ccgtacggcg gctgggccgg
     1321 cacaaacaac ccggcgagtt cgtgttcatc ccgaacaccc ccgatgcagc ccaagacgac
     1381 ggcgtgctca tgagcttcgt gaacgacgcc agcaccaacc gcagtgacct catgctgctc
     1441 gacgccgcca cgctcgagac catcgccgcg atccacctgc ccgagcgcgt cccgcacggc
     1501 ttccacggca actggtcacc cagctgcctc agtgaggcga accaggaggc gcatcagtga
     1561 ggcaaaccag ggagcccact gatcaccggc atcggccgcc gcggccagtc cgcagctcgc
     1621 cagctctcga gccatggatc ggtggcaatg gcaggcaaca gtccctgcca cagttctgtg
     1681 acggcctccc acaacgcgtt gtggccccgg aacaggtcac agagcaggtt agcgccggtg
     1741 atcatggaca cgacgacgcg ggcgatctgg ggcggatcga cgccgggccg cagcagccca
     1801 cctgccgcag cggcggtgag ttgctccttg gccgccgcct ctccgtagac gtagtgcgcg
     1861 gcagcgtctg cgcacaccac ccccggatca gtcagcaact ggatgccacc ccgggcgatc
     1921 ggatcttcga tcttgcggac cacgacttcg tcggtctgcg cgagcagcga ccacagtggg
     1981 tccaagccgc gggcgttgac ctcggcaacc atcacccgcc agcgcgcgac catctcggcc
     2041 accacggcat cagcgagagc ctgcttggaa gcgaagtgga agtacagcgc gcccttggtg
     2101 accttg
//
LOCUS       DATADN010000346         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_497242, whole genome shotgun sequence.
ACCESSION   DATADN010000346 DATADN010000000
VERSION     DATADN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497242"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..1425
                     /locus_tag="VHH53_04560"
     CDS             472..1425
                     /locus_tag="VHH53_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289465.1"
                     /translation="MLDIPQVQAEELVDTLVDMQLLDVAGRDATGALRYRFHDLLRAY
                     AREVVAAEETETDRLAALDRAFGGWLALAEEAGRCLASSAFAVTFEPGACWRPDESVV
                     SSVVTDPSGWFEAEWAALVGTVDQAYSVGSDQLGWALGVRLARFFLVREYYDDWRHVC
                     ERMLASAQRAASGGRERMALDGLGELSRLQHRLDEALEGFQQTLTACGATGNRRGHTV
                     ATWRKGAGPQPGHFDEALPRLEHALTYLRDLADRHSEVSTLRKLGTVHRLHRCYDKAA
                     RCFQQVLDALDQFGAPLRSGVRVSPGGLRLAALQGTPHAGW"
     gene            complement(1459..>2265)
                     /locus_tag="VHH53_04565"
     CDS             complement(1459..>2265)
                     /locus_tag="VHH53_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX2289466.1"
                     /translation="LDELYKAGAADLTEHPEVADLPCNTGLRLLAGTATALGRITADK
                     RVRLVRGDREVFGLRGRSAEQRVALDLLLDPAVGIVSLGGRAGTGKSALALCAGLDAV
                     MERGAHRRVVVFRPLYAVGGQELGYLPGNENEKMQPWAQAVFDTLGAVASQEVIDEVI
                     DRGMLEVLPLTHIRGRSLHDSFVIVDEAQSLERNVLLTVLSRLGTGSRVVLTHDVAQR
                     DNLRVGRHDGVAAVIEKLKGHPLFAHVTLTRSERSPIAALVTEMLEDYAH"
BASE COUNT          347 a          786 c          767 g          365 t
ORIGIN      
        1 caacgcggcg tgtgaggctc agctgcgacc gctgctgcca ggcaccgccg gctgcgcggt
       61 gctgatcacc agccggacgc gcctggccgg cctgaacggt gcccggatgg tcgacctgga
      121 catcctcgaa cccgaccagg ccgtcgagct gctggcccgg gttgctgggc cgctgcgggt
      181 ggcggccgaa cctggggcag ccagagagat cgtgcggctg tgcgggttcc tgccgttagc
      241 cgtccggatc gccggagccc ggctgggcgc ccgaccgcac tggcggttgg accgcctcga
      301 ggctgatctg gccgatgaac cccaccggct tgaccagctg cggctcggag atctggatgt
      361 gcgggccagc ctggcgcgga gtacgaaagc cttgatgcga cggcccggtg tgcgttccgc
      421 aggctggggc tgctggacgt ccgcgacttc acgccctgga tcaccgcagc gttgctggac
      481 atcccgcagg tccaggccga ggaactcgtc gacaccctgg tcgacatgca gctgctcgat
      541 gtggccgggc gtgatgccac cggggcgctg cgttaccgct tccatgatct gcttcgtgcc
      601 tacgcccggg aagtggtcgc cgcggaggaa accgagacgg accgcctcgc cgcgttggat
      661 cgggcgttcg ggggttggct ggccctggcc gaggaagccg gacgatgcct ggccagttcc
      721 gccttcgccg tgaccttcga acccggggcc tgctggcgac cagacgagtc agtggtcagc
      781 tctgtggtca ccgatccttc cggctggttc gaagccgaat gggcggcgct ggtgggcacc
      841 gtcgatcagg cttactcggt tggctcggat cagctcggct gggcgttggg cgtccgtctc
      901 gccagatttt ttctcgttcg cgagtactac gacgactggc ggcacgtctg tgagcgcatg
      961 ctcgccagcg cccagcgagc ggccagtggc ggccgggaga ggatggcgct ggacggtctg
     1021 ggtgagctgt cccggctcca gcatcgcctc gatgaggcgc tggaaggctt ccagcagact
     1081 ctcaccgcgt gcggcgccac cggtaaccgc cgcggccaca ccgtcgccac ctggcgcaag
     1141 ggtgccggtc cacaacccgg ccacttcgac gaagcattac cccgcctgga gcacgccctg
     1201 acctacctgc gcgacttggc cgaccgccac agcgaggtct cgacgctgcg caaactcggc
     1261 accgtgcacc ggctgcaccg atgctacgac aaagcggcgc gctgctttca gcaggttctg
     1321 gacgccctcg accagttcgg ggcaccccta cgcagcgggg tccgggtgtc gcccgggggc
     1381 ctgaggttgg cggctctgca aggcaccccg cacgcgggtt ggtagctctt gaaaagtcgc
     1441 cagtgtcctt ggcgccagtc agtgcgcgta gtcctctagc atctcggtaa ccaaggccgc
     1501 gatcggtgag cgctccgatc gggtgagggt gacatgggcg aacagcgggt gccccttcaa
     1561 cttctcgatc accgcggcga ctccatcgtg gcggcccacc cgcaggttgt ctcgctgcgc
     1621 gacgtcgtgg gtaagcacca cccgcgaccc agtgcccagc cgggacagca ccgtgagcaa
     1681 aacgttgcgc tccagggatt gggcctcgtc gacgatcaca aagctgtcgt gcagggaccg
     1741 gccccggatg tgagtcaacg gcagcacctc cagcatgccc cggtcgatca cttcgtcgat
     1801 cacctcttgc gaggcgaccg caccgagggt gtcgaacacc gcctgcgccc agggctgcat
     1861 cttctcgttc tcattgcccg gaagatagcc cagctcctga ccgccgacag cgtagagcgg
     1921 acggaagacc acgacccggc ggtgcgcgcc gcgctccatc acagcatcca gaccggcgca
     1981 cagcgcaagc gccgacttgc cggtccctgc cctgccgccc agtgacacga tgcccaccgc
     2041 gggatcgagc agcagatcca gcgcaacgcg ctgctcagct gagcggccgc gtaacccgaa
     2101 cacctcacga tccccgcgca ccagccggac ccgtttgtcc gcggtgatcc ggcccagcgc
     2161 cgtcgcggtt ccggcaagta gccgcaagcc ggtgttgcac ggcaggtcag caacctcggg
     2221 gtgctcggtg agatcggcgg ctcccgcctt gtagagctcg tcgag
//
LOCUS       DATADN010000347         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_643175, whole genome shotgun sequence.
ACCESSION   DATADN010000347 DATADN010000000
VERSION     DATADN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643175"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="VHH53_04570"
     CDS             <1..1613
                     /locus_tag="VHH53_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2289467.1"
                     /translation="HDERQVKTPAAVARPVIALAVASPAASLLDWPIERWVEFFDANH
                     SAKHGQNGQVAFLRYAYRCLEDLLCGTGWEAEFPRDVWELRRLGIEGRKRLRFDGIPQ
                     PWLRDLAKRFARWRLSIGRSPIQTYIDVQAVTRLAGFLASPPVNVTSLAGLDRAILER
                     YLADLAIDPRAIRTRSRDISSLSAFLEAIRRHEWDPDLPASAAFYPDDFPKPEKRLPR
                     GLAEHIMAQVEQPGNLDRWHSPGTRLLTVILMRCGLRIGDASRIAFDCMVRDGDGAPY
                     LRYVNRKMKREALVPIDEEIEQAILEQQQRVLRRWPSGGPWLFPAPKMNPDGRKPLST
                     HSYRGQLRDWLGRCDVRDEHGRPVHLTPHQWRHTFGTRLINRDVPQEVVRVLLDHSSG
                     EMTAHYARLHDTTVRRHWENARKVNAMGETVTIDPAGPLAEAAWAKQRLGRATQALPN
                     GFCGLPVQKTCPHANACLTCPMFVTTPEFLPQHREHRQQVLQIITAAEARGQQRLAEM
                     NQQVLGNLDNIITALEADTDGNEAADAS"
BASE COUNT          301 a          629 c          513 g          224 t
ORIGIN      
        1 ggcacgacga gcggcaggtc aagaccccgg ccgccgtcgc ccgcccagtc atcgcgctgg
       61 ctgtagccag cccggcggcc tcgctgctgg actggcctat tgagcggtgg gtcgagttct
      121 tcgacgccaa ccactccgcc aaacacggcc agaacgggca ggtcgcgttc ctgcgctacg
      181 cctaccgctg cctggaggac ctgctctgcg gcacagggtg ggaggccgag ttccccaggg
      241 acgtctggga actgcgccgg ctggggatcg agggccgcaa gcggctgagg ttcgacggca
      301 tcccacagcc gtggctgagg gatctggcga agcggttcgc tcgctggcgg ctgagcatcg
      361 gacgcagccc catccagacc tacatcgacg tccaagcggt gacccgactc gccggcttcc
      421 tggcgtcgcc cccagtgaac gtcaccagcc tggccggcct cgaccgcgcg atattggagc
      481 gctacctggc cgacctggcc atcgaccccc gagcgatccg cacccgcagc cgggatatca
      541 gctcactgag cgcgttcctc gaggcgatcc gccggcatga gtgggaccct gacctgcccg
      601 ccagcgccgc gttctacccc gacgacttcc ccaaaccgga gaagcgcctg ccgcgcggcc
      661 tggccgagca catcatggcc caggtcgagc agccgggaaa cctcgaccgc tggcacagcc
      721 ccggcacccg gctgctcacc gtcatcctga tgcgctgcgg actccggatc ggtgacgcca
      781 gcaggatcgc gttcgactgc atggtgcgcg acggcgacgg cgctccctac ctgcgctatg
      841 tcaaccggaa gatgaagcgg gaggcgctcg tcccgatcga cgaggaaatc gaacaggcca
      901 tccttgagca acagcaacgg gtcttgcggc gctggccgag tggcggccca tggctgttcc
      961 ccgccccgaa gatgaacccc gacggccgca aacccctgtc cacgcactcc taccgcggcc
     1021 agctgcgtga ctggctcggc cgctgcgacg tccgcgacga gcacggcagg cccgtgcatc
     1081 tcacacccca ccaatggcgc catacatttg gcactcggct gatcaaccgc gatgtccccc
     1141 aagaggtcgt gcgagtccta cttgatcatt ccagcggtga gatgacggcc cactatgccc
     1201 ggttgcacga caccaccgtc cgccggcact gggagaacgc ccgcaaagtc aacgccatgg
     1261 gcgagaccgt caccatcgac cccgccgggc cgctggccga ggccgcctgg gccaagcagc
     1321 gcctcggccg agccactcag gcactgccca acgggttctg cggattgccg gtccagaaga
     1381 cctgcccgca cgccaacgcc tgcctgacct gcccgatgtt cgtcaccacg ccggaattcc
     1441 tgccccagca ccgcgagcac cgccagcaag tcctgcagat catcaccgcc gccgaggccc
     1501 gaggccagca acggttggcc gagatgaacc aacaggtcct gggcaacctc gacaacatca
     1561 tcaccgcgct ggaggccgac accgacggga acgaggcggc cgatgcgagc tgacaacacc
     1621 cgccacatca tcgccgccgc ccaacgacgc cacgagctca cccgggc
//
LOCUS       DATADN010000348         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_497646, whole genome shotgun sequence.
ACCESSION   DATADN010000348 DATADN010000000
VERSION     DATADN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497646"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..>1556)
                     /locus_tag="VHH53_04575"
     CDS             complement(244..>1556)
                     /locus_tag="VHH53_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX2289468.1"
                     /translation="SERIWRAAFDQAGVLAARSGLEMLDAARALDSQPPPAGNRIAII
                     TNSGGTGVELADLLADEGLTVPELSTALQEELRALLPPLASPANPVDITPVWRRFAEL
                     YPCLVDRLARSGEVDVVIPVLLQRAATDQAVATGVRDVVDRLRDDGVTTPVYVCWVAP
                     RSARANAEPLQEAGVPCFEWPERTARAVGHAVSYLNPRAPRVRHSEPFGGAQGVLGRH
                     SEPFGGAQILAPDLGARIVSEAGIGVVESITCRDVTEAVTAAQRIGYPVVCKVVHPDL
                     VHRSDVGGVRLNLNGPDAIQTAALALLALADGAQVQVQPQLGGVEVAVGGGRDPQFGP
                     FVMIGLGGILVEVMDDVAFGLAPLSSGEAHRLIESLRGYTVLAGARGREPVDLDALAG
                     AVSAVGDLLATRPEIIDLDLNPLLATPGGAVTVDWHITVEDIKR"
BASE COUNT          262 a          534 c          528 g          232 t
ORIGIN      
        1 tagacggcat aggcgtggcc gtcgtaccag cgcgggttgg gatagttgcc caccatggtg
       61 gtgggtagga gcacgtcctg tgatccgatg cgcacgcctg tctcctcctc tcgaggggcg
      121 gcgtcgcggc gcgaaccgcc cgtcccatga tgtgacggcg accacagtag ggatcaccgc
      181 cgggcaatgc tccgtgatcc cgggccaaac ctcgaggcgt gggctcgtcc tggcaggacc
      241 gattcagcgt tttatgtcct cgaccgtgat gtgccagtcc accgtcacgg cgccgcctgg
      301 agtagccagc agcgggttca ggtcaaggtc aatgatctcg ggccgggtgg cgaggaggtc
      361 gccgaccgcg gagactgcgc cggcgagcgc gtcgaggtct accggttcgc ggccgcgggc
      421 tccggcgagg acggtgtagc ctcgcaggct ctcgatcaat cggtgtgctt cgccactgga
      481 cagcggggca agaccgaatg ccacatcgtc catcacctcg accaggatcc cgccgaggcc
      541 gatcatgacg aacgggccga actggggatc acgcccaccg ccgacggcga cctcaacccc
      601 gccgagctgc ggttggacct ggacctgggc gccgtcggcg agcgcgagta gcgcaagcgc
      661 cgccgtctgg atcgcatcag gaccgttgag attcagccgg actccgccga cgtcactgcg
      721 gtgaaccagg tctgggtgca ccactttgca caccaccgga tagccgatgc gctgcgccgc
      781 ggtgaccgcc tcggtcacat ccctacaagt gatcgactca accaccccga tccctgcctc
      841 actcactatc cgggcaccta gatcaggtgc gagtatctgg gcacccccaa aaggttccga
      901 atggcgaccg agaacaccct gcgcacctcc aaaaggttcc gaatggcgga cgcggggggc
      961 gcgggggttg aggtaggaga ctgcatgacc cacggcgcgg gcggtgcgct ctggccactc
     1021 gaagcagggc accccggcct cttgcagcgg ctcggcattg gctcgcgcgc tgcgtggcgc
     1081 cacccagcac acgtagaccg gcgtggtcac cccatcgtca cgcagccggt ccaccacgtc
     1141 gcgcacgccc gtagcgacgg cctgatcggt ggctgcgcgc tgcagcagga cggggatgac
     1201 cacgtccacc tcaccggagc gcgccagccg gtccaccagg cacgggtaca gctcggcgaa
     1261 gcgccgccac accggggtga tgtcgaccgg gttggccggg ctggccagcg gcggtaaaag
     1321 ggccctcagc tcttcctgca gtgcggtgga cagctcgggc acggtcaggc cttcgtcggc
     1381 gagcaggtca gcgagctcaa ccccggtacc accggagttc gtaatgatcg cgatccggtt
     1441 accggcaggc ggcggctggg aatccagcgc gcgggcggcg tcgagcatct ccaaccctga
     1501 gcgggccgcc agcacgccgg cctggtcgaa tgcggctcgc cagatccgct cgctac
//
LOCUS       DATADN010000349         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_643438, whole genome shotgun sequence.
ACCESSION   DATADN010000349 DATADN010000000
VERSION     DATADN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643438"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VHH53_04580"
     CDS             <1..820
                     /locus_tag="VHH53_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2289469.1"
                     /translation="AADRMLGRLIKVTPSSKVVGDLALHLVGAGVEPTDFEADPARFD
                     IPDSVIGFLHGELGDPPGGWPEPLRSKALKGRSDPKGIAELSAEDRKGLREDRARTLN
                     RLLFAGPTADFEEHRESYGDTSVLPSKEFFYGLRSGQEHAVDLEPGVRLLIQLQAIGD
                     ADERGLRTVMCTLNGQLRPLQIRDHSIDSEIPVAERANKSDSNHVAAPFAGVVTLQVS
                     EGDTVSAGQLVATIEAMKMEAGITAPKDATVARLAIKTVQQVEGGDLILELRSS"
     gene            859..1413
                     /gene="rsmD"
                     /locus_tag="VHH53_04585"
     CDS             859..1413
                     /gene="rsmD"
                     /locus_tag="VHH53_04585"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX2289470.1"
                     /translation="MVRIVAGVAGGRRLVVPPHGTRPTSDRVREAMFSALQARHDLNG
                     SRVIDLYAGSGALGLEALSRGAVHVRFVESDRRAAAVLRRNVEALGLPGAQVTTGPVQ
                     AVLSGDPGDPYDLVLADPPYTLDEAALAGVLSALANGGWLARSALLVVERPARALEPT
                     WPGDVVALTHRRYGDTTVYYGCAP"
     gene            1410..>1632
                     /locus_tag="VHH53_04590"
     CDS             1410..>1632
                     /locus_tag="VHH53_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX2289471.1"
                     /translation="MRRAVCPGSYDPATNGHLDIIERAAAQFDEVVVAVLINKTKSCL
                     FEVSDRMAMLKEITEHLAGVRVDSWHGLLV"
BASE COUNT          260 a          517 c          573 g          282 t
ORIGIN      
        1 agcggctgac cggatgctcg gcaggttgat caaggtgacc ccttcctcca aggtcgtggg
       61 agatctggcg ctgcacctgg tcggtgccgg tgtcgagccg acagacttcg aggccgatcc
      121 ggctcggttc gacatccccg actcggtgat cggattcctg cacggcgaac tcggtgatcc
      181 tcccggcggc tggcccgaac ccctgcgcag caaggcgctc aagggtcgca gcgaccccaa
      241 ggggatcgcg gagctgtccg ccgaggaccg caagggcctt cgcgaggacc gggcgcgcac
      301 cctcaaccgg ctgctgttcg ctggtcccac cgcagacttc gaagagcatc gcgagtccta
      361 cggggatacc tcggtactgc ccagcaagga gttcttctac gggctgcgtt ccggtcagga
      421 gcatgcggta gatctcgaac cgggggtgcg gctgctgatc cagcttcagg cgatcggcga
      481 tgctgacgaa cgcggcctgc gcaccgtgat gtgcaccctc aacgggcagt tgcggccact
      541 gcagatccgg gatcactcca tcgactcgga gatcccggtt gcagagcgag ccaacaaatc
      601 cgacagcaac catgttgcgg cgccgttcgc gggggtggtc accctgcagg tgtccgaagg
      661 ggacaccgtg tctgccgggc agctggtggc caccatcgag gcgatgaaga tggaagccgg
      721 catcaccgcg cccaaggacg ccacggtggc ccgcctggcc atcaagactg tgcagcaggt
      781 ggaaggtggc gacctgatct tggagcttcg ctcctcgtga gtggcgttga gtgctatagc
      841 cctgctattc actcacgagt ggtccggatc gtggcggggg tagcgggtgg gcggcggctg
      901 gttgtgccgc cgcacggcac ccggccgaca tcggatcggg tccgggaggc gatgttcagt
      961 gcgctgcaag cccgccacga cctcaacggt tcccgggtaa tcgatctcta cgccggatcg
     1021 ggggcattgg ggttggaggc gctgtcccgc ggagcggtgc acgtccggtt cgtagagtcc
     1081 gatcggcgcg cggcggccgt gctgcgccgc aatgtcgagg cgctcgggtt gccaggggcg
     1141 caggtcacca ccggcccagt gcaggcggtg ctgtccggtg atcccggcga cccctatgac
     1201 ctggtgctcg ccgacccgcc ctacacactg gacgaggccg ctctggccgg tgtgttgagc
     1261 gccttggcca acggcggttg gctggcccgc tcggcgctgc tggtggtaga gcgcccggcc
     1321 agggcacttg agccgacctg gccaggcgat gtggtggcgc tcacgcatcg aaggtatggc
     1381 gacaccacgg tgtactacgg ttgcgcgcca tgaggcgcgc ggtgtgtccc ggctcgtatg
     1441 accccgcaac caacggtcac ctcgacatca tcgaacgggc cgctgcccag ttcgacgagg
     1501 tcgtggtcgc tgtcctgatc aacaagacca agagctgcct gtttgaagtc tccgaccgga
     1561 tggcgatgtt gaaggagatc accgagcacc tcgcaggcgt tcgggtcgat tcctggcacg
     1621 ggctgctcgt gg
//
LOCUS       DATADN010000350         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1402416, whole genome shotgun sequence.
ACCESSION   DATADN010000350 DATADN010000000
VERSION     DATADN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402416"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VHH53_04595"
     CDS             <1..658
                     /locus_tag="VHH53_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HEX2289472.1"
                     /translation="DAIIDVAQNAHGLRVQRLYGRSIVNAFHGVWSVGGVTGAVLGSV
                     AVPVTGGWDTFVDVTAALSNVPAATTTLYLVFTGSGTGALFDVDAFTFAGGTGPITGI
                     GGKCVDVSGANPADGTNIQLWTCNGTAAQTWTRSGSTLRALGKCMDVSGGATADNTRV
                     QLWTCNSTAAQNWTPQTNGTLVNPQSGKCLTPSGGGTADGTQLVILTCTGASNQRWTM
                     "
     gene            726..>1774
                     /locus_tag="VHH53_04600"
     CDS             726..>1774
                     /locus_tag="VHH53_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX2289473.1"
                     /translation="MKRSLGVATTALLILACSSAPPANAADTPYDVLVFSRTVGFRHD
                     SIPIGTQTIRDLGAANTFSVTATEDPNAFNSGNLAQYEAVVFLNTTGDVLNASQQTAF
                     ESYIRGGGGYVGVHSAADTEYDWPFYGNLVGAYFASHPAIQQATVRVENRAHQATQHL
                     GSSWVRTDEWYNYRTNARSTARVLATLDESTYSGGSMGADHPHTWCKTIQSGRSFYTG
                     NGHTQASYSDSSFRSLLLGGIRYAANKTKADCRPETGYTTLYNGSTTGWSQAGPGSFT
                     NSNGTLTSVGGLGLLWYSAREFRSYSLKLDWMMPGDDNSGVIVGFPATSDPNQALSQG
                     YEVQIDATDTPDRTTG"
BASE COUNT          300 a          660 c          565 g          249 t
ORIGIN      
        1 cgacgccatc atcgacgtcg cccagaacgc ccatggcctc agggtgcagc ggctctatgg
       61 gcgctcgatc gtcaacgcct tccacggcgt ctggagcgtc ggcggcgtca ccggtgccgt
      121 gctcggctcg gtggccgtgc cggtgaccgg cgggtgggac accttcgtcg acgtgacggc
      181 ggccctgtcc aacgtcccgg ccgcgaccac cacgctctac ctggtgttca ccgggagcgg
      241 cacgggcgcg ctgttcgacg tggacgcgtt cacgttcgcc ggcggcaccg ggccgatcac
      301 cggcatcggc ggcaagtgcg tggacgtgtc cggcgccaac cccgccgacg gcacgaacat
      361 ccagctgtgg acctgcaacg gcaccgcagc ccagacctgg acccggtccg gctcgaccct
      421 gcgcgcgctg ggcaagtgca tggacgtctc cggcggcgcc accgccgaca acacccgcgt
      481 ccaactctgg acctgcaaca gcaccgcggc ccagaactgg accccgcaga ccaacggcac
      541 gctggtcaac ccgcagtcgg gcaagtgcct gacgccgtcg ggcggcggca ccgccgacgg
      601 cacccaactg gtgatcctga cctgtaccgg cgccagcaac cagcgctgga cgatgtaaga
      661 gcctgttggc atcccttccc gctcgggaaa gggaggccac atcgcttccc tccaggaggc
      721 ttgtgatgaa gcgatccctc ggcgtggcca ccactgcgct gctcatcctg gcgtgcagct
      781 ccgcgccacc ggccaatgcc gctgacaccc cgtacgacgt cctggtgttc tcccggacgg
      841 tggggttccg gcacgattcc atcccgatcg gcacccagac catccgggat ctgggcgccg
      901 ccaacacgtt ctcggtgacc gccaccgagg acccgaacgc gttcaactcg ggcaacctgg
      961 cccagtacga ggcggtggtg ttcctcaaca ccaccggcga cgtgctcaac gccagccagc
     1021 agaccgcctt cgagtcctac atccggggcg gtggcggcta cgtcggggtg cactccgccg
     1081 ccgacaccga gtacgactgg ccgttctacg gcaacctggt cggggcgtac ttcgcctcgc
     1141 acccggcgat ccagcaggcg accgtgcggg tggagaaccg ggcgcaccag gccacccagc
     1201 acctcgggtc gtcctgggtg cgcaccgacg agtggtacaa ctaccgcacc aacgcccggt
     1261 ccaccgcacg cgtactggcc actttggacg agtcgacgta ctcgggtggc tcgatgggcg
     1321 ccgaccaccc gcacacctgg tgcaagacga tccaaagtgg acggtcgttc tacacgggca
     1381 acgggcacac ccaggcgtcg tactccgact cgtcgttccg cagcctgctg ctcggcggca
     1441 tccggtacgc ggccaacaag accaaggccg actgccgccc ggagaccggg tacaccacgc
     1501 tgtacaacgg ttccacgacc ggctggtcgc aggccgggcc gggcagcttc accaacagca
     1561 acggcacgct gacctcggtc ggcggactcg gcctgctgtg gtacagcgcc cgggagttcc
     1621 ggtcgtactc gctcaagctg gactggatga tgcccggtga cgacaactcc ggcgtgatcg
     1681 tgggcttccc ggccaccagc gacccgaacc aggccctcag ccaagggtac gaggtgcaga
     1741 tcgacgccac cgacacgccg gaccgcacca ccgg
//
LOCUS       DATADN010000351         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1102247, whole genome shotgun sequence.
ACCESSION   DATADN010000351 DATADN010000000
VERSION     DATADN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102247"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1281
                     /locus_tag="VHH53_04605"
     CDS             319..1281
                     /locus_tag="VHH53_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2289474.1"
                     /translation="MPSTLDVEPRTRPEDAAAAGGRPRAARKKRSWGGVTPYLFLAPW
                     FAGLLFITAGPIVASAYLSFTDYSLIGAPEWTGLENYQRMLDDPRFYQSLKVTFVYVL
                     VSVPLQLAFALFLAMVLDRGLRGLALYRSIFYLPSLLGGSVAIAILWRQIFGSEGLLN
                     GVLQFLGVENPPGWVSDPDYALGTLIILNVWTFGAPMVIFLAGLRQIPTMYYEAASLD
                     GAGKVRQFFAVTLPLLSPIIFFNLVLQIINAFQTFTQAFIVSGGTGGPADSTLFYTLY
                     LYQRGFGNFDMGYASALAWVLLLIVGGLTAVNFIASKYWVFYGD"
     gene            1271..2161
                     /locus_tag="VHH53_04610"
     CDS             1271..2161
                     /locus_tag="VHH53_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2289475.1"
                     /translation="MATDLSVAEPVAGTAALPPRREKRRPRYLAHLGLIAFAALMLYP
                     LLWMVSSSFKFTEDIFSQTGLIPERVTGQNYPEGWTALPRDFGHYILNSTVIVAGSII
                     GNLLACSLAAYAFARLEFRFKKFWFAVMLGTMMLPIHVVIVPQYILFSQLDWINTYWP
                     LIVPKLLATDAFFVFLMVQFIRGLPRELDEAARIDGCGHGRIFWRIILPLMKPALATT
                     VIFTFIWTWNDFFSQLIFLTDPDMYTVPVALRTFVDATSQTPWGPVFAMSIVALGPIF
                     GFFLAGQRYLVRGIATTGLK"
BASE COUNT          320 a          796 c          744 g          431 t
ORIGIN      
        1 ctgcgacgcg gccgcggacg cggtgaccga acgatagacc cgaacgccca gctcatcgcg
       61 gggttgtgcc gctccggacg agacgtcggc gggcgcaccg gtcgtccatc gggaggtcgc
      121 cgctgaccgg gatatgagaa agcgcttacc ctactgtgac cagggacacg cgccacgccg
      181 tcccgcgaga gggcgttgac actgctccgg acacccgcta gaaagcgctt gccgcgttgt
      241 gaggcagcgc acagagcccc atcccgagct ccatcccgga ccgatcccac gcgagccccc
      301 cgagagaggt gagagcccgt gccgtccacg ctcgacgtcg agccacgcac gcgtcccgag
      361 gacgcggccg cggccggcgg gcgaccccgc gccgcaagga agaagcgctc ctggggcgga
      421 gtgacgccct acctcttcct ggccccgtgg ttcgccggcc tgctgttcat cacggcgggg
      481 ccgatcgtcg cctccgccta cctgtccttc accgactaca gcctcatcgg cgcgccggag
      541 tggacggggc tggagaacta ccagcggatg ctggacgacc cgcgcttcta ccagtccctg
      601 aaggtcacct tcgtctacgt gctggtgtcg gtcccgttgc agctggcctt cgccctgttc
      661 ctggcgatgg tgctcgaccg cgggctgcgc ggcctggccc tctaccgctc gatcttctac
      721 ctgccgtccc tgctgggtgg cagcgtcgcc atcgcgatcc tctggcggca gatcttcggc
      781 tccgagggcc tgctgaacgg ggtgctgcag ttcctcggtg tggagaaccc gcccgggtgg
      841 gtctcggacc cggactacgc gctgggcacc ctgatcatcc tgaacgtctg gacgttcggt
      901 gcgccgatgg tcatcttcct ggccggtctc cggcagatcc cgaccatgta ctacgaggcg
      961 gcgtccctgg acggggccgg caaggtgcgc cagttcttcg cggtcaccct gccgctgctc
     1021 tcgccgatca tcttcttcaa cctggtcctg cagatcatca atgccttcca gaccttcacc
     1081 caggcgttca tcgtcagcgg gggcacgggt ggtccggccg attccacgct gttctacacg
     1141 ctgtacctgt accagcgcgg gttcggcaac ttcgacatgg gctacgcgtc agctctcgcg
     1201 tgggtcctcc tcctcatcgt cggcggcctg acggccgtca acttcatcgc ctcgaagtat
     1261 tgggtgttct atggcgactg acctgtccgt ggccgaaccc gtcgccggta cggccgccct
     1321 gccaccacgc agggagaaga ggcggccccg ttatctcgcg cacctggggc tcatcgcatt
     1381 cgcagcgctc atgctgtacc cgctgctgtg gatggtctcg agctcgttca agttcaccga
     1441 ggacatcttc agccagaccg ggctgatccc ggagcgcgtc accgggcaga actaccccga
     1501 gggctggacg gcgctgcccc gggacttcgg ccactacatc ctgaactcga cggtgatcgt
     1561 cgccggctcc atcatcggca acctgctggc ctgctcgctg gccgcgtacg cgttcgcccg
     1621 gctggagttc aggttcaaga agttctggtt cgcggtgatg ctcggcacga tgatgctgcc
     1681 gatccacgtg gtgatcgtgc cgcagtacat cctgttctcg cagctggact ggatcaacac
     1741 ctactggccg ctgatcgtgc cgaagctgct cgccaccgac gcgttcttcg tgttcctgat
     1801 ggtgcagttc atccgcggcc tgccgcggga gctggacgag gcggcgcgga tcgacggctg
     1861 cggccacggg cggatcttct ggcggatcat cctgccgctg atgaagccgg ccctggccac
     1921 cacggtgatc ttcacgttca tctggacctg gaacgacttc ttcagccagc tgatcttcct
     1981 caccgacccg gacatgtaca cggtgccggt ggcgctgcgg acgttcgttg acgccaccag
     2041 ccagaccccg tggggtccgg tcttcgcgat gtccatcgtg gccctcgggc cgatcttcgg
     2101 gttcttcctc gccgggcagc gctacctcgt ccgaggcatc gcgaccaccg gcctgaagtg
     2161 aagggagctg cgtcatggtc cggcgggcgc tcgtggccgc gggggcggcc gtcgcactgc
     2221 tgttgagcgg ctgcggcggc gggtccgacg agggtggtcc ggtcacgctg cggttctcgt
     2281 ggtgggggag c
//
LOCUS       DATADN010000352         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_184513, whole genome shotgun sequence.
ACCESSION   DATADN010000352 DATADN010000000
VERSION     DATADN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184513"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VHH53_04615"
     CDS             complement(<1..642)
                     /locus_tag="VHH53_04615"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEX2289476.1"
                     /translation="MPEFVIGERDFLLDGRPIQLVSGALHYFRVHPDQWADRIDKARR
                     LGLNTIETYVAWNAHAPKPGMFDLTGGLDLDRFLRLVAEAGMHAIVRPGPYICAEWDN
                     GGLPAWLFRDPAVGVRRYESRYLAAVREYLAEALEVVAPHQIDRGGPVVLVQIENEYG
                     AFGADKRYLAALVEYTRAAGITVPLTTVDQADDEMLAAGGLDGVHRTVSFGSHA"
     gene            complement(650..>1894)
                     /locus_tag="VHH53_04620"
     CDS             complement(650..>1894)
                     /locus_tag="VHH53_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX2289477.1"
                     /translation="QPAGGSPDAVDAALKAGGEITYWTWTPQAEEQVAAFQQEHPNVK
                     VNLVNAGTGNDHYTKLQNAIMAGSGAPDVAQIEYQALPQFALTDALVDLNQYGFGAFE
                     PDYTPSTWASSKVGNGLYGLPQDSGPMALFYNKQVFDQYGIAVPKTWDEYLAAARTLH
                     AADPTKYIANDNGDAGFATSMIWQAGGRPFATDGRSVTINLQDDGSKKWTAVWNQLLR
                     EKLLLQADAWTDEWYRALGNGTVATVVTGAWMPGVLESSVADGKGKWAVAPVPSYDGQ
                     PVTGENGGSAQSVLKQSKNPALAAGFVRWLNHDEDSIKVFLDAGGFPATTADLRSATF
                     RDKPSDYFGGQQINQVLTAAADNVVEGWSYLPYQLYANSIFPDTVGKAYLNGSDINAG
                     LLDWQQALVDYGNQQGFTVQAG"
BASE COUNT          266 a          661 c          647 g          320 t
ORIGIN      
        1 ggcgtgggat ccgaacgaca cggttcggtg cacgccgtcc aggccgcccg cggcgagcat
       61 ctcgtcgtcg gcctggtcca ctgtggtcag tgggacggtg atcccggcgg cgcgggtgta
      121 ctccaccagg gccgccaggt accgcttgtc ggcaccgaag gcgccgtact cgttctcgat
      181 ctggaccagc acgaccgggc cgccgcggtc gatctggtgc ggcgcgacca cctcgagcgc
      241 ctccgcgagg tactcgcgga cggccgccag gtaccgggat tcgtagcggc ggacgccgac
      301 cgcgggatcg cggaacagcc aggcgggcag gccgccgttg tcccactcgg cgcagatgta
      361 cgggcccggc cgcacgatcg cgtgcatgcc ggcctcggcg accaggcgca ggaaccggtc
      421 cagatccaac ccgccggtca ggtcgaacat gcccggtttg ggcgcgtgcg cgttccacgc
      481 gacgtaggtc tcgatcgtgt tcagccccaa ccgccgcgcc ttgtcgatgc ggtcggccca
      541 ctgatcggga tgcacgcgga agtagtgcag cgcaccggaa acaagctgga tcggacggcc
      601 gtcgagcagg aagtcccgct cgccgatgac gaactcgggc atcgccgcct cagccggcct
      661 ggaccgtgaa gccctgctgg ttgccgtagt ccaccagcgc ctgctgccag tccaggagcc
      721 cggcgttgat gtccgagccg ttcaggtacg ccttgcccac cgtgtccggg aagatgctgt
      781 tggcgtagag ctggtacggc aggtagctcc agccctccac cacgttgtcc gcggccgcgg
      841 tgagcacctg gttgatctgc tgcccgccga agtagtccga cggcttgtcc cggaacgtgg
      901 ccgaccgcag gtctgccgtg gtggccggga aaccgccggc gtccaggaag accttgatgc
      961 tgtcctcgtc gtggttgagc cagcgcacga acccggcggc cagggcggga ttcttgctct
     1021 gcttgagcac cgactgcgcg gatccgccgt tctcaccggt gaccggctgc ccgtcgtagc
     1081 tcggcaccgg cgccacggcc cacttgccct tcccgtcggc caccgaggat tccagcacgc
     1141 cgggcatcca tgccccggtg accacggtgg caacggttcc gttgcccagc gcccggtacc
     1201 actcatcggt ccaggcgtcg gcctgcagca gcagcttctc ccgcagcagc tggttccaca
     1261 ccgccgtcca cttcttggac ccgtcgtcct gcaggttgat cgtgaccgac cggccgtcgg
     1321 tggcaaacgg ccggccaccg gcctgccaga tcatgctcgt ggcgaacccg gcgtccccgt
     1381 tgtcgttggc gatgtacttg gtcgggtcgg ccgcgtgcag cgtgcgggcc gcggcgaggt
     1441 actcgtccca ggttttgggg accgcgatcc cgtactggtc gaacacctgc ttgttgtaga
     1501 acagggccat cggcccggag tcctgcggca ggccgtacag gccgttgccc accttcgacg
     1561 aggcccacgt ggacggtgtg tagtccggct cgaacgcgcc gaacccgtac tggttgagat
     1621 cgaccagcgc atcggtcagg gcgaactggg ggagggcctg atactcgatc tgcgcgacat
     1681 ccggcgcgcc ggacccggcc atgatcgcgt tctgcagctt ggtgtagtgg tcgttgccgg
     1741 tgccggcgtt gaccaggttc accttgacgt tcggatgctc ctgctggaac gcggcgacct
     1801 gctcctcggc ctgcggggtc caggtccagt aggtgatctc accgcccgcc ttcagcgccg
     1861 cgtcgaccgc gtccggtgag ccgccggccg gctg
//
LOCUS       DATADN010000353         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1402636, whole genome shotgun sequence.
ACCESSION   DATADN010000353 DATADN010000000
VERSION     DATADN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402636"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="VHH53_04625"
     CDS             complement(<1..1669)
                     /locus_tag="VHH53_04625"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX2289478.1"
                     /translation="MRATARGAVLMVLCIMAHLAAPASPVVAHAFLESSDPIANAVLP
                     TAPQSITMTFTEPLETSYSRAELFDETGAQVPGAISTIGPQPRTMTVEIPPGLGNGTY
                     SLLWRTLSTVDGHTAQGYLPFTVGTEADVRVVAPPAVATTSGALPEWTLAAARWLALL
                     GMAMVVAVWPVWLLVVRPAISPAWQLGPKLTRRVRRYATAAFAFSLLANLVALAVQAA
                     AISGPADLLTGLTTTLGDTRYGTWWLVRVGVLLVFAAVLLGTAWWWPWRRRPATLLAL
                     VASAALPLPFSMISHAGAEPEGQATAVAFNYVHLLGASLWAGGLLFLVVALAPTVGDL
                     TAAGRRVVLGRALPRFSVLALIAWSVMGFTGLYSAWLQVGNIPALTGTPYGQTLILKL
                     ILIVPLLALGAFNLAVVTRKLRSAETEERVDGWSNHLVSALIAEAVIVTLLLGVVGML
                     IGTPPARQVMEQQAGSLRIALDAAGQTGTLIITPGTVGPNHYRLELGSGHEAHLRNPA
                     VTDATLRLSLPERQTGQIDVPLITAPSGGYEAHGSGLAFPGDWQMQLT"
BASE COUNT          306 a          567 c          571 g          240 t
ORIGIN      
        1 ccgtcaattg catctgccag tcaccgggga acgccagccc cgacccgtgc gcttcgtatc
       61 ctccagatgg tgcggtgatc agcggcacgt cgatctgccc cgtctggcgc tctggcaggc
      121 taaggcggag cgtggcgtcg gtgacagccg ggttgcgcag gtgcgcctcg tgtccactgc
      181 cgagctccag ccggtagtga ttcggaccta ccgttcccgg cgtgatgatc aacgtgccgg
      241 tctggccagc ggcatcgaga gcgatccgca ggctgccggc ttgctgctcc atcacctggc
      301 gtgccggtgg cgttccaatc agcattccga cgacaccgag gaggagcgtg acgatgaccg
      361 cttccgcgat caaggcactg acgaggtgat tgctccaccc atccacccgc tcctcggttt
      421 cggccgagcg cagcttgcgc gtgacgactg ccaggttgaa cgcaccgagc gcgagcaggg
      481 gcacgatcag gatcagcttc aggatgagcg tctgcccgta cggggtcccg gtgagggccg
      541 ggatgttgcc gacctggagc cacgccgagt agagtccggt gaaccccatg acgctccagg
      601 cgatcaatgc cagaactgag aagcgcggca gggcgcgacc cagaaccaca cgccgcccgg
      661 ccgcggtgag atcgcccacg gtcggcgcca aagcgacgac gaggaagaga agtcctcccg
      721 cccagagcga cgcgccgagg aggtgcacgt agttgaaggc aaccgccgtc gcctgcccct
      781 cgggctcggc cccggcgtgc gagatcatcg agaagggcag tggcagcgcc gcgctggcga
      841 caagggctaa cagggtcgcc ggccgcctcc gccacggcca ccaccaggca gttcccaaga
      901 ggaccgcggc gaagacaagg aggacaccaa cccgcaccag ccaccaggtg ccatagcggg
      961 tatcaccgag tgtcgtggtc agaccggtga ggagatccgc cggcccggag atagccgccg
     1021 cctgcaccgc cagcgccacg aggttggcca gcagggagaa ggcgaatgca gctgtcgcat
     1081 atctccgcac ccgccgcgtc agttttggac ctagttgcca ggccggggag atcgccggcc
     1141 gcacgacgag gagccagacc ggccacacgg cgaccaccat cgccatgccg agtaacgcca
     1201 gccagcgggc ggccgccaac gtccattccg gcagcgcgcc tgatgtggtg gcgaccgccg
     1261 gcggcgctac aacgcggaca tcggcttcgg taccgacagt aaagggcaaa tacccctgcg
     1321 cggtgtgccc gtcgacggtc gagagggtgc gccagagaag cgaataggtg ccattaccga
     1381 gccccggcgg aatctcgacc gtcatcgtcc gtggctgcgg tccgatagtg ctgatcgcgc
     1441 ctggaacctg cgctcccgtc tcgtcgaaca actcggctcg cgaatacgag gtctccagcg
     1501 gctcggtgaa ggtcatggtg atcgactggg gagcggtcgg cagcacggcg ttggcgatcg
     1561 gatcgctgct ttcgagaaac gcgtgcgcta ccacggggct cgccggcgcc gcgaggtgcg
     1621 ccatgatgca gagcaccatc aggacggccc cgcgcgccgt cgcgcgcacc ttggctcggt
     1681 tcat
//
LOCUS       DATADN010000354         2616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1102850, whole genome shotgun sequence.
ACCESSION   DATADN010000354 DATADN010000000
VERSION     DATADN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102850"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VHH53_04630"
     CDS             complement(<1..1088)
                     /locus_tag="VHH53_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEX2289479.1"
                     /translation="MSGDSTGVGTGRPGSPWPTPGALVGKGRYRLLSEVGRDDRGGVR
                     LWRGRDLVLDRDVALTLFIAPPQDSGAVMVIRSAVARALRSARLEAAGAARVLDVLEP
                     DARFGPTVAVVVAEWTRGRGLVELLDDGLPPPAMAAGVLAPLAGAVDAAHSAGLILGC
                     DHPDRIRVTHDRQARLAFPGPLPMTGSQDDIRGLGAMLYLLLTGYWPLPGGPSTLPPA
                     PRQPDGSLVSPTTLRHGVPVELSTLALRSLADPGTGGGVHTGAAVREVLELNASSADD
                     RNGRHSRTPPTSPEQARRQRRVKLGVSMAVLATITLMILGYGGMQVLSVFVNAGGTPL
                     VVASPSPGEQSEPPPVVARSSPVQVKNLA"
     gene            complement(1225..>2616)
                     /locus_tag="VHH53_04635"
     CDS             complement(1225..>2616)
                     /locus_tag="VHH53_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX2289480.1"
                     /translation="VTLACATLGVATVLAVAAAPFLAALLLGDGLRADPQLATIFAYL
                     LLPQILFYGLSGLFGAILQARSVFGPPAWAPVLNNLIVLVTLGIYVLVPGEITINPAR
                     ISDPKLLVLGLGTTAGIIAQALVQLPGLRRAGFQFRWRWGWDPRLTQAGGMAAWFMLY
                     VLVGQLGFVVTTRAAAQGEPGGVVIYSVVWLLLQLPYGVLGYSLLTAIMPRMSRAAAD
                     ADWRGVVKDLSLGSRYLTVLLIPITVIFTIAGQQIGIALFSLGKAGSDAERLGAALAV
                     SAFGLLPFAVTMLQLRVFYSMADARTPTMINALMVVVKLPLLLACPVLLDDADVVLGL
                     TAVNSLSCVLGAMVGQAWLRRRLGRVETKRTLITLRNCLLAAVPAVGAVLGLRALLDL
                     DGSSGAVWGAWLDLVLTTVVVGVVMGGVLIALRTPELTEAIRASIGARRAPVLAQGTR
                     RPPEQPPDPPKEP"
BASE COUNT          476 a          951 c          864 g          325 t
ORIGIN      
        1 gccaaattct tgacctggac cggcgaggac cgcgccacca ccggcggcgg ctcggactgc
       61 tcgcccggtg acgggctggc gacgaccagc ggggtacccc cagcgttgac gaacaccgac
      121 agcacctgca tgccgccgta accgaggatc atcagcgtga tggtggccag caccgccatc
      181 gacacgccca gcttcacccg gcgctggcgg cgggcctgct cggggctggt cggcggggtg
      241 cgactgtgcc ggccattgcg gtcgtcggcg ctggacgcgt tgagctcgag cacttcccgc
      301 acggcggcac cggtgtgcac gccgccgccg gtgcctggat cagcgagact gcgcagcgcc
      361 agggtggaca gctccaccgg cacaccgtgg cgcagcgttg tggggctgac cagggagccg
      421 tcgggttgcc gcggtgcggg gggcagggtg ctcggtccac caggcagcgg ccagtatcca
      481 gtgagcaaca ggtacagcat cgcgcccaga ccacggatgt cgtcttggga cccggtcatg
      541 ggaagcgggc cgggaaatgc gagacgcgcc tgcctgtcgt gagtcaccct gatccgatcc
      601 ggatggtcgc agcccaggat cagaccggcg ctgtgtgcgg cgtccaccgc accggccagc
      661 ggggccaaga caccggctgc catcgccggc ggcggcagcc cgtcatccag gagctcgacc
      721 aggccacgac cccgggtcca ctccgcgacc acgaccgcga cggtcgggcc gaacctcgcg
      781 tcgggctcca gcacatcgag cacccgcgcc gcaccagccg cctccaaacg agccgaacgc
      841 aacgcgcgcg ccacggcgga gcggatcacc atgaccgcac cggagtcctg cggcggcgcg
      901 atgaacaagg tcagcgcgac gtcacgatcc agtaccaggt cgcggccccg ccacagccgc
      961 acgccgccac ggtcgtcgcg gcccacctcg gacagcaagc ggtaacgacc cttgccgaca
     1021 agcgcgccgg gagtcggcca cggggagccg ggccggccgg tccccacccc cgtcgagtca
     1081 ccgctcaccg gaccgatcaa cctccccgtg ttcttccgct gctccgggtt cagcagcaca
     1141 gctttgcccc gacgcaacac cccgacacaa caccgcacca cgcgagacgc aacgcgcgag
     1201 gtatgggtga ggtaattgtc aagtctacgg ctccttgggg ggatctgggg gttgttcggg
     1261 cggccgccgg gtcccctgcg ccagtacggg tgcccggcgc gccccgatcg aagcccggat
     1321 cgcctcggtg agctccgggg tgcgcagtgc tatcaggaca ccacccatca ccacgccgac
     1381 gacgaccgtg gtgagcacca ggtccagcca tgctccccag acagcgccac tgctgccatc
     1441 gaggtcgagc agggcacgca acccgagcac cgcacccact gccggcaccg ccgccagcag
     1501 gcaattgcga agggtgatca gtgtgcgctt ggtttccacc cggcccagcc gccggcgcag
     1561 ccacgcctgc ccgaccatcg cgcccagcac gcacgacaga gagttgaccg cggtgagtcc
     1621 gagcacgaca tcggcgtcgt cgagcagcac cgggcaggcc agcaacagcg gcagtttcac
     1681 caccaccatc aatgcgttga tcatggttgg tgtgcgggca tcggccatcg agtagaaaac
     1741 ccgcaattgc agcatcgtca ccgcaaacgg cagcaggccg aaggccgaca ccgccagcgc
     1801 agcgccgagg cgctcggcat cgctgcccgc cttgcccagt gagaacagcg cgatcccgat
     1861 ctgctgcccg gcgatggtga agatcactgt gatcgggatc agcagcaccg tcaggtagcg
     1921 gctacccagc gagaggtcct tgaccactcc ccgccagtcg gcgtccgccg cggcccggct
     1981 catccgcggc atgatcgccg tgagcagcga gtaaccgagc acgccgtagg gcagttgcag
     2041 cagcagccac accacgctgt aaatcaccac tccacccggt tcgccctgcg ccgccgcccg
     2101 cgtggtcacc acgaagccga gctggccgac cagcacgtag agcatgaacc aggccgccat
     2161 cccgccggct tgggtcagcc tgggatccca gccccatcgc cagcggaact gaaagcccgc
     2221 tcggcgcagg ccgggcagct gcaccagcgc ctgcgcgatg atgcccgcgg tcgtccccag
     2281 gcctaggacc agcagcttcg ggtcgctgat ccgcgctggg ttaatggtga tctcgcctgg
     2341 taccaggaca tagataccga gagtcaccag cacgatgagg ttgttgagca cgggcgccca
     2401 ggccggtgga ccgaagacgc tacgtgcctg aagaatcgcc ccgaacagcc cggacaagcc
     2461 atagaacagg atctgcggca gcagcaggta ggcgaatatg gttgccagct gcgggtcggc
     2521 gcgcagcccg tcgccgagca acagcgcggc cagaaacggc gccgcggcga cagcgagcac
     2581 cgtggccaca ccgagcgttg cgcaggcgag ggtgac
//
LOCUS       DATADN010000355         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_350416, whole genome shotgun sequence.
ACCESSION   DATADN010000355 DATADN010000000
VERSION     DATADN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350416"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            875..2392
                     /locus_tag="VHH53_04640"
     CDS             875..2392
                     /locus_tag="VHH53_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX2289481.1"
                     /translation="MSTGLIDPDFLDLPLRSAADAALGKAHDLGAEHADIRIERTTRQ
                     LLRLRDGAVATAQDSSTVGLAVRVIVEGTWGFASHLELSPQSAVATAQRAVEVARTLR
                     RLNAERVELADEPVHANAFWVGDHEIDPFTVPLAEKVALLSEWSGRLLATDGVEHTDV
                     LLWAEKETKFYADSAGTSTTQQRVRMHPAVTAIAVDRAAGSFESMRTCAPPTARGWEY
                     LAGNTIWNWDDELAQIPEWLTEKVKAPSVPPGCYDLVIDPTNLWLTIHESVGHATEYD
                     RAIGYEAAYAGSSFATPDKLGTLRYGSSAMHVTGDRTQPHGLATIGYDDDGVATSQWD
                     LVAGGTLVGYQTDRVFARRLGLDRSAGCAYADSPHHVPIQRMANVSLQPDPRADTTTS
                     DLISAVDRGIYIVGNRSWSIDMQRYNFQFTGQRFYRIEGGRLAGQLRDVAYQATTTDF
                     WGAMEAVGGPSTWLLAGAMNCGKAQPGQVAAVSHGCPSALFRGINVLNAREEAGQ"
     gene            2392..>2635
                     /locus_tag="VHH53_04645"
     CDS             2392..>2635
                     /locus_tag="VHH53_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX2289482.1"
                     /translation="MIGAVELTEAALAASKADGCIVLLTDTSEATLRWANNTMTTNGH
                     STTRSLSVISVVEAAAGSAVGPAMGVVSGNGADLDEV"
BASE COUNT          418 a          869 c          900 g          448 t
ORIGIN      
        1 ccctcatgtg cttggcggcg cggtacggca ccgtagtgat tgtcggtcga ggcagccaga
       61 cggtgagatc gtgtcccccc gatgctcacc gtctggctgc ctcgaccgac aatcactaca
      121 gtgccctacc gcgccgccag gcacatgagg gttcccccct accgggaccc tggttcaaca
      181 ccatgactgc ccgaagagga catgaaccgt ccatggtgag cggcgatgca cgagcgccgt
      241 atgaaccgcg cccttgaggc agcgtggccc gcatcatccg ggctgctgag ctgccccagc
      301 cacctgcgaa ttgcctcgtc gacgaatggc gtgctgacag ccccacggtg ctgcgggatt
      361 gctgggcagc gcggtagtga tcgcctctgt tgtgctcctc acggcgcccg cggcagtcac
      421 gatggatacg caggcgatga gggcggcatg ggatctttcg ggtctccttg agtgagctta
      481 ctcatcgcag tggtggcggt tggcacaagc agcgtggcca tattgcgtgg cggtgccctg
      541 ccgaggtggc tcgggtactg cggattggcc gtcacattcg cccggttggt actggaaatc
      601 acacagattg tcagcggagc ctatgttgag ggccctcgca atattgtgtt ctttgcgttc
      661 ctgatttgga gatcggtgcg acgagcatcg tgctggtgtc gacgattcgc cgatcgccaa
      721 tcggctggcc gtcgctacga cgatccgggt cctggccccg tcgcggcagt gaagagcggc
      781 cagcagcgcc gtgcggtggt cacagcagcg agcccgctgc tgcggcgctg ggccagcggc
      841 tggtgcagcg aacgcccggc tagggtcggt gggtgtgagc accggcctga tcgatcctga
      901 tttccttgac ctgccgttgc ggtcagccgc cgacgccgcc ctgggcaaag cccacgatct
      961 gggtgccgag cacgccgaca ttcggatcga gcggaccacc cggcagctgc tgcggctgcg
     1021 tgacggggcg gtggcgaccg cgcaggactc gtccaccgtc gggctggccg tgcgggtgat
     1081 cgtcgagggc acctgggggt tcgcctccca tctcgagctg tccccgcagt cggccgtggc
     1141 caccgcgcag cgggccgtcg aggtggcccg cacgctgcgc cggctcaacg ccgagcgggt
     1201 ggagctggct gatgagccag tgcacgccaa cgccttctgg gtcggggacc acgagatcga
     1261 cccgttcacg gtgccgctgg ccgagaaggt ggcactgctg tcggagtggt ctggccggtt
     1321 gctcgccacc gacggcgtcg agcacaccga tgtgctgctc tgggcggaga aggagaccaa
     1381 gttctacgcc gactccgccg gcaccagcac cactcagcag cgagtacgga tgcatccggc
     1441 ggtcaccgcg atcgcggtgg accgggccgc cggatcgttc gagagcatgc ggacctgcgc
     1501 gccgccgact gcccgagggt gggagtacct cgccggcaac accatctgga actgggacga
     1561 cgagctcgcg cagattccgg aatggttgac tgagaaggtc aaggcaccca gcgtgccgcc
     1621 cggctgctac gacctggtga tcgatcccac caacctgtgg ttgacgattc acgagtcggt
     1681 gggccacgcc accgaatacg accgggcgat cggctacgag gccgcctatg ccgggtcctc
     1741 cttcgccact ccggacaagc tcggcacctt gcgttacggc tcgtcggcga tgcatgtcac
     1801 cggtgaccgc actcagccgc acgggctggc caccatcggc tacgacgacg acggggtcgc
     1861 cacctcgcag tgggacctgg tggccggcgg gacgctggtc ggctaccaga ccgatcgggt
     1921 gttcgcgcgc cggctggggt tggaccgctc ggcgggctgc gcctacgccg actcaccgca
     1981 ccacgtgccc attcagcgga tggccaatgt gtccctgcag cctgatcccc gcgccgacac
     2041 cacgacgtct gacctgatct cggcggttga ccgtggtatc tacatcgtcg gcaaccgctc
     2101 ctggtcgatc gatatgcagc gatacaactt ccagttcacc gggcagcgtt tctaccggat
     2161 tgagggcggc aggctcgccg ggcagctccg agacgtcgcc taccaggcca ccaccacgga
     2221 cttctggggc gcgatggagg ctgtgggtgg cccctcgacg tggttactgg ccggcgcgat
     2281 gaactgcggc aaggctcaac ctggccaggt cgcggcagtc agtcacgggt gcccctccgc
     2341 attgttccgc ggtatcaacg tgctcaacgc ccgcgaggag gccggccagt gatgatcggg
     2401 gctgtcgagc tgaccgaggc cgcgctggcc gcgtcgaaag ccgacggatg catcgtgctg
     2461 ctcaccgaca ccagtgaggc caccctgcgt tgggccaaca acacgatgac caccaacggg
     2521 cacagcacga cccgctcctt gtcggtgatc tcggtggtgg aagcggccgc aggatccgcc
     2581 gtgggccccg caatgggcgt tgtcagcggc aacggtgctg acctcgacga ggtgc
//
LOCUS       DATADN010000356         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_498710, whole genome shotgun sequence.
ACCESSION   DATADN010000356 DATADN010000000
VERSION     DATADN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498710"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VHH53_04650"
     CDS             complement(<1..987)
                     /locus_tag="VHH53_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2289483.1"
                     /translation="MVQTDTDALAALRAVETELDRRWPESRIEPSLARIAALVDVLGM
                     PQRCYPALHVAGTNGKTSVARMLDALLDRIGLRVGRYTSPHLQLVTERISLHGEPISP
                     ERYVEAYRDIERYVAMVDTGSQVPMSKFEVLTGMAFATFADAPVDAAVVEVGLGGSWD
                     ATNVADATVATITPVAVDHVDYLGSDIAGIAAEKAGIIKPGSVAVLAEQPPEVAKVIA
                     ARSIEVDATVAREGMEFGVLHRDIAVGGQRLRLQGLGGVYDEIFLPLHGEHQGHNAVL
                     ALAAAEAFFGAGASRSLDIDAVRDAFATVTAPGRLERVRTAPSVLLDGAHNPH"
     gene            1196..>1833
                     /locus_tag="VHH53_04655"
     CDS             1196..>1833
                     /locus_tag="VHH53_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2289484.1"
                     /translation="MTSEPHFVRVPDRVSVDGLEAKWVPVWENEHVYRFDRTKTREQI
                     YSIDTPPPTVSGSLHVGHVFSYTHTDTMARYQRMRGREVFYPMGWDDNGLPTERRVQN
                     YFGVRCDPSLPYDPSFTPPDKPGKNVVACSRQNFIELCEQLTAIDEQAFEEVWRRLGL
                     SIDWSTTYQTIDGIARAISQRAFLRNLARGEAYQSEAPTLWDVTFRTAVAQA"
BASE COUNT          329 a          640 c          560 g          304 t
ORIGIN      
        1 atgcggattg tgtgcgccat cgagcaacac cgagggtgcc gtgcggaccc gttccagccg
       61 tcccggcgcg gtcaccgtcg cgaaggcgtc gcgcaccgcg tcgatgtcca gcgaccggct
      121 ggcgcctgca ccgaagaacg cctcggcagc ggccagagcc agcacggcgt tgtgcccctg
      181 gtgctcgccg tgcagcggca ggaagatctc gtcgtacacc cctccgagac cctgcagccg
      241 caaccgctgc ccgccgacgg cgatgtcgcg gtgcagcaca ccgaattcca tgccttctct
      301 ggccaccgtc gcgtccacct cgatactgcg tgccgcgatg accttggcca cctcgggtgg
      361 ttgctcagcg agtaccgcca ccgaacccgg cttgatgatc ccggccttct ccgcggcgat
      421 gcccgcgatg tcgctgccca ggtaatcgac gtgatccacc gcgaccggag tgatcgtcgc
      481 caccgtggcg tcggcgacgt tggtcgcgtc ccagctgcca ccgaggccga cttcgacgac
      541 agcggcgtcc accggcgcat cggcgaaggt cgcgaaggcc atcccggtca gcacctcgaa
      601 cttactcatc gggacctgtg atccggtatc gaccatggcc acataccgct cgatgtcccg
      661 gtaggcctcg acgtaacgct ccggactgat cggctcgccg tgcaggctga ttcgctcggt
      721 gacaagctgc aggtgaggac tggtgtagcg acctacccga agcccgatcc tgtccagcag
      781 cgcatccagc atgcgagcca ctgaggtctt gccattggtg ccggcgacat gcagcgccgg
      841 gtaacaccgc tgcggcatac cgagcacgtc gaccagcgcc gctatccggg ccaacgaggg
      901 ctcgatccgg ctctccggcc agcgacggtc gagctcggtc tcgactgccc gcaacgcagc
      961 gagcgcatcg gtatcggtct ggaccacgcc gctcaggccc ccgatgcagc gacatcagcc
     1021 cgtgcgcggt cacacggggc accgatgtcg taaatcgagt ggtgaacgga aggctgagtc
     1081 atcgccacat cacgagtcta cggagcagcc atctcctccg acggtgacac cggcggcccc
     1141 gagatcggtg ggtcacccag cgttcggctt tcctgccccg aatacgatga acgctatgac
     1201 gtccgaaccc cacttcgtcc gcgtgccaga ccgcgtctcc gtcgacgggc tggaggccaa
     1261 gtgggttccg gtgtgggaga acgagcacgt ctaccgcttc gaccggacga agacacggga
     1321 gcagatctac tcgatcgaca ccccaccgcc gacggtgagc ggctccctgc acgtgggcca
     1381 cgtgttttcc tacactcaca ccgacaccat ggcgcgctac caacggatgc ggggtcgcga
     1441 ggtcttctac cccatgggct gggacgacaa tggcctaccc acggaacggc gagtgcagaa
     1501 ttatttcggg gtgcggtgcg acccatcgtt gccctatgac ccgagcttta ccccgcccga
     1561 caaacctggc aagaatgtcg tggcctgctc acgtcaaaac ttcatcgaac tgtgcgaaca
     1621 gcttactgca atcgatgagc aggcgttcga ggaggtgtgg cgtcgcctcg ggctctcaat
     1681 cgactggtcc acgacgtacc agaccattga cgggatcgcg cgggctatct cgcaacgtgc
     1741 ctttctgcgc aaccttgccc gcggagaggc ctaccaatcc gaagcgccga cgctgtggga
     1801 cgtcaccttc cggaccgccg tcgctcaagc aga
//
LOCUS       DATADN010000357         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1404024, whole genome shotgun sequence.
ACCESSION   DATADN010000357 DATADN010000000
VERSION     DATADN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404024"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="VHH53_04660"
     CDS             complement(<1..105)
                     /locus_tag="VHH53_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2289485.1"
                     /translation="MSTALITGPTAGLGAAFARRLAAEGYDLVLVARDL"
     gene            complement(115..1302)
                     /locus_tag="VHH53_04665"
     CDS             complement(115..1302)
                     /locus_tag="VHH53_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX2289486.1"
                     /translation="MAIAANAVSARSLAPDLARGAMLLLIALANAHLYLYGRPVGVRG
                     YPIPESTADRLVVLVQMTFVDGRAYPMFAFLFGYGIVQLWRRHTRKGVNEVAVGRLVR
                     RRGCLMILFGFLHALLLFSGDIIGAYGLLAVVLAGLLIRAGDEKLLILAGLWTVPIMA
                     FGVLQALPAPEGSESLLPSMSITDPLAAAGVRAIEWIQLTVVLSFLGLVPAVALGVWA
                     GRRRLLDEPERHRPMLVGVAVLGVGLAAAGGLPMALLAASWWPDPSVMTTALAGVAHA
                     LTGYAGGLGYAAIAGLIAIRTRNRYRPGPVSTALAACGQRSMTCYLLQSVVFVAVLAA
                     YGGGLGNRLGLATTAALATVTWALTVLLADAMARLGYRGPAELLLRRLSYRRSPQPRS
                     GES"
     gene            complement(1396..>1681)
                     /locus_tag="VHH53_04670"
     CDS             complement(1396..>1681)
                     /locus_tag="VHH53_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289487.1"
                     /translation="ESLGLVGERAAFVTDPARMRSMITPELVFACDAVGGDVPVIWME
                     QSWVLAAVPAATTPTRLERLLRALDEIADLLDGDDTAATSQRARVTDFAD"
BASE COUNT          319 a          583 c          577 g          202 t
ORIGIN      
        1 aaggtcgcga gcgacgagca cgaggtcgta accctcagcg gccagcctgc gcgcgaacgc
       61 ggcccccagc ccagcggtgg gcccagtaat cagcgcagtg ctcatggttc gaggctagct
      121 ctctcccgaa cgcggttggg gggaccggcg gtaggagagg cggcgaagga gtagctcggc
      181 tgggccccgg taaccgagac gggccattgc gtcggccagc agcacggtca gcgcccaggt
      241 cactgtggcc agggcggcgg tggtcgcgag ccccaatcgg ttaccgaggc cgccgccgta
      301 ggcagccagc accgccacga acaccacgga ctgcagcagg tagcaggtca tcgatcgctg
      361 cccgcaggct gccagcgcgg tgctcaccgg accgggacgg tagcggttgc gggtccggat
      421 ggcgatgagc ccggcgatcg cggcgtaacc cagcccaccg gcgtagccgg tcagggcatg
      481 cgccacgccc gcgagcgcgg tcgtcatcac cgacggatcg ggccaccatg aggccgccag
      541 cagcgccatc ggcagaccac cggcggcggc cagcccgacg ccgagcaccg cgacaccgac
      601 cagcattggc cggtgccgct cgggctcgtc gagcagccgc cgccgccccg cccagactcc
      661 cagcgccacg gcgggtacca ggccgaggaa cgacagcacg acagtgagct ggatccattc
      721 gatggcccgg actccggctg cggccagcgg atcggtgatg gacatcgacg gcagcaatga
      781 ctccgaacct tcgggcgccg gcagggcctg cagcacaccg aaagccatga tcggcactgt
      841 ccacaagccc gccaggatca gtagcttctc gtcaccggcc cggatcagca gcccggccag
      901 cacgacggcg agcaagccgt aggcaccgat gatgtcgccg gagaacagca gtagcgcgtg
      961 caggaacccg aagaggatca tcaaacagcc gcgcctgcga acgagcctgc cgacggccac
     1021 ctcgttgaca cccttgcggg tgtgccgacg ccacagctgc acgatgccgt agccgaacaa
     1081 gaaggcgaac atcgggtacg cgcggccgtc cacaaaggtc atctggacca gcacgaccag
     1141 ccgatccgcg gtcgactcgg ggatggggta accgcgcacg ccgacagggc gcccgtacag
     1201 gtagaggtga gcgttggcca gcgcgatcag cagcagcatt gcgccccgcg ccaggtccgg
     1261 cgcgagcgag cgcgccgata ccgcgtttgc ggcgatcgcc actagcccga cccgctggtg
     1321 gatgcaatga tcagcgcagc gggcatggtc cgacgctagt accccggtag gtgagtcggc
     1381 gaagcaagac ctcagctagt ccgcgaagtc ggtgactctt gcacgttggc tggtcgctgc
     1441 ggtgtcgtca ccgtcgagca gatcggcgat ttcgtcgaga gcgcgtagca ggcgctccag
     1501 ccgggtgggc gtggtggcgg ccggcaccgc ggcaagcacc caactctgct ccatccagat
     1561 gaccgggaca tccccgccca ccgcatcaca ggcgaagacc aattcgggag tgatcatcga
     1621 gcgcatccga gccgggtcgg tgacgaaggc ggcccgctca cccaccagac cgagcgattc
     1681 c
//
LOCUS       DATADN010000358         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_498872, whole genome shotgun sequence.
ACCESSION   DATADN010000358 DATADN010000000
VERSION     DATADN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498872"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="eno"
                     /locus_tag="VHH53_04675"
     CDS             <1..1047
                     /gene="eno"
                     /locus_tag="VHH53_04675"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX2289488.1"
                     /translation="EAIEQRVVDQKLVDLDGTPHKSRLGANAILGASLAVAKAAAESS
                     GLELFRYIGGANAHVLPVPMLNILNGGAHADTGVDVQEFMIAPIGADSFREALRWGTE
                     CYHALKSVLKSNGLATGLGDEGGFAPDLPTTTDALDLIASAVNKAGYTLGHDIVLALD
                     VAATEFFADGSYTFDKRTRSAEQMAIYYAELLDAYPLASIEDPLAEDDWDGWARLNAE
                     IGDRVQLVGDDLFVTNPERLEEGISRRVANALLVKVNQIGTLSETLDAVTLAQSSGYR
                     CMMSHRSGETEDTTIADLAVAIGCGQIKTGAPARSERVAKYNQLLRIEEALGDAARYA
                     GDLAFARFTPEGPA"
     gene            1064..1513
                     /locus_tag="VHH53_04680"
     CDS             1064..1513
                     /locus_tag="VHH53_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX2289489.1"
                     /translation="MGDPGPSRSAALGGSVTHPLGVRICRFLGITSPRRAAMLAAVVC
                     VLALSVAVPLRNYVSQRSELAAVQEQQLIMADKVAELERRRALLSDPQHVQAQARERL
                     RYVRPGEAPYVVQLPPGQGAATVTGTGNAVAPRELWYGRLWRSINSG"
     gene            1527..1997
                     /locus_tag="VHH53_04685"
     CDS             1527..1997
                     /locus_tag="VHH53_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEX2289490.1"
                     /translation="MSATDQEAVAAQLGRPPRALRAVAHRCACGLPSVVQTHPRLEDG
                     TPFPTLYYLTCRRLAALVSRREASGAMREMTDRLAADPELAAAYRVAHESYLAERDAV
                     EPLGTDVSAGGMPERVKCLHVHVAHALASGPGVNPFGDEVLDQIGPWWTPGPCT"
     gene            2110..>2335
                     /locus_tag="VHH53_04690"
     CDS             2110..>2335
                     /locus_tag="VHH53_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289491.1"
                     /translation="MGQRMGADPRGQHAHDLVGGAPAKPRAEERLGHHRHGALGRGSA
                     RPRRQPQQCGPDALHGGGHADYQRGRGSLFT"
BASE COUNT          369 a          791 c          800 g          375 t
ORIGIN      
        1 gaggcgatcg agcagcgcgt ggtggatcag aagttggtcg atctggacgg cacgccgcac
       61 aagtcccggc tgggtgccaa tgcgattctc ggggcctcgc tcgcagtcgc caaggcggcc
      121 gccgaatcca gcggtctgga gttgttccgc tacatcggtg gcgccaacgc gcatgtgttg
      181 ccggtaccga tgctgaacat tctcaacggg ggcgcgcatg ccgacaccgg cgtcgacgtg
      241 caggagttca tgatcgcccc gatcggtgca gactcgttcc gggaggcgct tcgctggggc
      301 accgagtgct accacgcgct gaagtcggtg ctcaagtcca acgggttggc aaccggactc
      361 ggtgacgagg gcggtttcgc ccccgacctc ccgaccacta ccgacgcgct ggacctcatc
      421 gcctccgctg tgaacaaagc cggttacacg ctcggtcacg acatcgtgct tgcgctagac
      481 gttgctgcca ccgagttctt cgccgacggc tcctacacct tcgacaagag gactcgcagt
      541 gccgagcaga tggccatcta ctacgccgaa ctgctcgacg cctacccgct ggcgtccatt
      601 gaggacccgc tcgcggagga cgattgggat ggctgggcgc ggctcaacgc ggagatcggc
      661 gatcgggtac agctcgttgg tgatgatctg ttcgtgacca atccggagcg gctggaggag
      721 ggcatcagcc gccgggtagc caacgcgcta ctggtgaagg tcaaccagat cggcacgctg
      781 tcggagacct tggacgcggt aacacttgcc cagtccagcg ggtaccgctg catgatgagt
      841 caccgctccg gggagaccga ggacaccacg attgcagatc tcgcagtggc gatcggctgc
      901 ggtcagatca agactggtgc acccgcccgg tcggaacggg tagcgaagta caaccagttg
      961 ctgcgcatcg aggaggctct cggagacgcg gcccgctatg ccggggacct ggcgttcgcc
     1021 cggttcactc cggagggccc agcttgatat gaggcccagc tcgatgggcg atcccgggcc
     1081 aagccgcagc gctgcgctcg gtggctcggt gacccatccg ctgggcgtac ggatctgtcg
     1141 cttcctgggc atcacctctc cacgccgagc cgcgatgctc gccgcggtgg tctgtgtgct
     1201 ggcgttgagc gttgccgtac cgctgcgcaa ctacgtcagc cagcgctccg agctcgccgc
     1261 ggtgcaagaa cagcaactga tcatggcgga caaggttgcg gagctggaac ggcgccgcgc
     1321 gctgctctcg gacccgcagc acgtccaggc gcaggctcgc gaacggttgc gctacgtgcg
     1381 gcccggggag gcgccgtacg tggttcagct gccccccggc cagggcgctg ccactgtcac
     1441 cggtaccggc aatgcggtgg cgcccaggga gctgtggtac ggccggctct ggaggtcaat
     1501 caacagtgga tgatctccgt ccggagatgt cggccaccga ccaggaggcc gtcgccgcac
     1561 agctgggccg cccaccgcgt gcgctgcgag ctgtcgcgca ccgctgcgcc tgcgggttgc
     1621 ccagcgtggt tcagacccat ccccggctgg aggacggcac cccgttcccc accctctact
     1681 acttgacctg tcgccggttg gccgcactgg tgagccgcag ggaggctagc ggggcgatgc
     1741 gggagatgac tgatcggttg gctgcggatc ccgagctggc tgcggcgtac cgggtcgcgc
     1801 acgagtcgta cctggccgaa cgagatgcgg tggagccgct cgggaccgac gtcagcgccg
     1861 ggggaatgcc ggagcgggtc aagtgcctgc acgtccacgt cgcgcatgca ctggccagcg
     1921 ggcctggcgt gaaccctttc ggcgacgagg tgctcgacca gatcggaccc tggtggaccc
     1981 ccggcccctg cacctaacgc ccaacccacc cccctccggc gtgtcgcgct cagctgcccc
     2041 gcgtgtcagg ctcaactgcc cggtgtgccc gcgtctggtg ctcacgcccc gggggcccgg
     2101 agtaatccga tgggccaacg catgggtgcc gatccccgcg ggcagcacgc ccacgatctg
     2161 gtcggcggtg cccccgccaa gccgcgagct gaggaacgcc ttggccacca ccgccacggc
     2221 gcgcttggca gaggtagtgc tcgcccgcga cgccagccgc agcaatgtgg ccccgatgcg
     2281 ctccacggag gcggccatgc cgattaccag cgcggtcggg gctcgctgtt caccg
//
LOCUS       DATADN010000359         3944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1104162, whole genome shotgun sequence.
ACCESSION   DATADN010000359 DATADN010000000
VERSION     DATADN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104162"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2440
                     /locus_tag="VHH53_04695"
     CDS             <1..2440
                     /locus_tag="VHH53_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX2289492.1"
                     /translation="GTLLGGLALATAFEVARRLAIEVDVRRDTARIAPTEIPLVIGLL
                     FLPGPVVLAAYVGVVVIARLIRRDTWPKLLFNISYATLTVALTDLSARLVFQSVWPGM
                     PQWSAVLAGFVFAHAVTACSAWAFFVLLDRRRPGESARPVVHIYLTGLLNAALGLAAA
                     QIVLTVPWGALLVGLLVVIVVAVYSAYYGLLRDQRDLGVLNDLSLRVAAAGKSEDGGH
                     SKPDGQPKPDEDGWSAAMELAREQLNATRVVLHWTPDEGQPHTVVAGRPLPRPLSQSD
                     VAVLTPADRQAGTGQVLHLLERGCDPAICQALTARSAAEVLVVPLRGANRLLGIIEVH
                     DRQSRLRGFGAADVRLVETLASHLTTALDNRRLLARLRHDAYHDSLTNLRSRLGFREA
                     ATEVLRRDSRSCAVIVIDLQLVSSVNDALGHVWGDRVIIGAGERLRRTLPASALTARL
                     EGDTFAALLVDVDSQRAVQVAEQVRAVLSQPYPVDKLAVECSAVAGVALTEIETTRDG
                     DTLLQRADMALRAARSADETVRCYVPSMGQLLLRRFQLVTQFRHALDAGQFDVHFQPQ
                     LALASRQVTGVEALVRWHHPELGMLDPEEFVAIVEATGLIDPLTDFVLDRSLQQCRSW
                     LDRGLSLSVAVNLSVRNLADTAFPSRVADALARHQVSAALLCLELTESAVMSDPERAM
                     PVLRGLHGLGVRIAVDDFGTGYSSLAYLRRLPVDEVKIDKSFVLGLASDLGDLAVVRA
                     IVELGHSLGLIVIAEGVEQDVTRDQLAEMGCDLAQGYLISRPLQVDRFDSWLAARTVC
                     VPGAHDETVLTLVR"
     gene            2541..2613
                     /locus_tag="VHH53_04700"
     tRNA            2541..2613
                     /locus_tag="VHH53_04700"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2574..2576,aa:Thr,seq:ggt)
     gene            2678..2753
                     /locus_tag="VHH53_04705"
     tRNA            2678..2753
                     /locus_tag="VHH53_04705"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2711..2713,aa:Met,seq:cat)
     gene            2846..2995
                     /gene="rpmG"
                     /locus_tag="VHH53_04710"
     CDS             2846..2995
                     /gene="rpmG"
                     /locus_tag="VHH53_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX2289493.1"
                     /translation="MRPKITLACEECKHRNYITNKNRRNDPDRLTIRKYCPNCNKHRA
                     HRETR"
     gene            3027..3500
                     /locus_tag="VHH53_04715"
     CDS             3027..3500
                     /locus_tag="VHH53_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2289494.1"
                     /translation="MALDPSVVGRSYPAADTYQVGREKIREFAEAIGDDNTVYRDPDA
                     ARSFGYPDVIAPPTFAIILALRAQQALINDPDLGLDYSRMVHGDQAFIHHRPILAGDE
                     LATTLHVDGVRTMGGNDLLTVRCEITDRAGDPVTTARSLLVVRGPDKEPDGEQDR"
     gene            3497..3922
                     /locus_tag="VHH53_04720"
     CDS             3497..3922
                     /locus_tag="VHH53_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX2289495.1"
                     /translation="MTRRRRTEVAEGEELPVLQVSVTRAELIRYAGASGDFNPIHWNE
                     RVAREVGLPDVIAHGMLTMALANRLVTGWAGDPGAIVECGVRFTRPVVVPDDGAGALV
                     ELSGTVSQRRDDGTVVVAITAWAGGQTVLGKATATVALA"
BASE COUNT          602 a         1323 c         1330 g          689 t
ORIGIN      
        1 tggcacgcta ctcgggggtc tcgcgctggc gacggcattc gaggtcgcac ggcgcctggc
       61 gatcgaggtc gacgttcgtc gcgataccgc acggatcgcg cccacggaaa ttcccctggt
      121 catcggtctg ctcttcctac cgggcccggt tgtgctggcg gcctatgtcg gtgttgtcgt
      181 catcgcccgg ctcatccgac gtgacacctg gcccaaactg ctgttcaaca tctcctatgc
      241 gaccctcacc gtcgcgttga ccgacctgtc ggcgcgcctg gtcttccagt cggtgtggcc
      301 cggaatgccg cagtggagcg cggtactcgc cggattcgtc ttcgcccacg ccgtcaccgc
      361 gtgctcggcc tgggcattct tcgtgttgct cgaccgcagg cgtcccggtg aaagtgcgcg
      421 gccggtggtg cacatctacc tcaccggtct actcaacgcc gcgctcgggc tcgccgcggc
      481 gcagatagtg ctgacggtgc cctggggcgc cctgctggtc ggcctgctgg tggtgatcgt
      541 ggtcgcggtg tacagcgcct attacgggct gctgcgtgac cagcgtgatc tcggtgtgct
      601 caacgacctc agcctgcggg tggcggcggc gggcaagagt gaggacggcg gccactctaa
      661 acctgacggc cagcccaaac ctgatgagga cggctggagc gctgccatgg agctggctcg
      721 tgagcagctc aacgccaccc gggtggtgtt gcactggaca ccggacgagg gccagccaca
      781 caccgtggtt gccggtcggc cgctgccccg tccactgtcg caatccgacg tcgccgtcct
      841 gacgcccgcc gaccggcagg ctggcacggg gcaggtgctg cacctactgg aacgtggttg
      901 tgacccggcc atctgccaag cgctgaccgc caggtcagcg gctgaggtgc tggtggtccc
      961 gctgcggggc gccaaccggc tgctcggcat catcgaggta catgatcggc agagtcgcct
     1021 gcgcggcttc ggggcggcgg atgtgcggtt ggtcgagacg ctggccagcc acctcaccac
     1081 cgcgctggac aatcgccggt tgctcgccag gctccggcac gacgcctacc acgacagcct
     1141 caccaacctc cgtagccgcc tcggcttccg ggaggctgcg accgaggtgc tgcgccggga
     1201 ctcgagatcg tgcgcagtga tcgtcatcga tctccaactg gtgtcgtcgg tgaacgatgc
     1261 gctgggccac gtctggggcg accgcgtcat catcggtgcg ggcgagcggc tccgccgcac
     1321 cctgccggcc tcagcgctga ccgcccgcct ggagggggac acgttcgccg ccctgctggt
     1381 ggacgtcgac tcgcagcggg cggtgcaggt cgccgagcag gtgcgggcgg tgctgagcca
     1441 gccgtatccg gtcgacaagc tcgccgtgga gtgcagcgcg gttgccggcg tggcactcac
     1501 cgagatcgaa accacccgcg acggggacac cttgctgcag cgggctgaca tggcgctgcg
     1561 tgcagcgcgc agtgccgatg agaccgtccg ctgctacgta cccagcatgg gccagcttct
     1621 gctgcgccgt ttccagctcg tcacgcagtt ccggcacgcg ctggacgctg ggcagttcga
     1681 cgtgcacttc cagccccagc tggcgttggc cagccgtcag gtgactgggg tggaggcgct
     1741 ggtgcgctgg caccatccgg agctagggat gctcgaccct gaagagttcg tcgccatcgt
     1801 cgaggccacc ggcctgatcg acccgctcac cgacttcgtg ctcgaccgtt cgctgcagca
     1861 gtgccggagc tggctggacc gtgggctgtc actgtcagtg gcggtcaacc tttcggtgcg
     1921 caacctggcc gacaccgcgt ttcccagccg ggtggccgac gctctggcgc gccatcaggt
     1981 gtcggcggcc ttgctgtgtt tggagctcac cgagtcggcg gtgatgagtg atcccgagcg
     2041 ggcgatgccg gtgctgcgcg ggctgcacgg tcttggcgtc cgtatcgcgg tggacgactt
     2101 cggtactggg tactcctccc tggcctacct gcggcgcctt ccggtggatg aagtcaagat
     2161 cgacaagagt ttcgtgctcg gactggccag tgacctcggt gacctcgcgg tggtccgcgc
     2221 catcgtcgag ctcgggcact cgcttggatt gatcgtcatc gccgagggtg tcgagcaaga
     2281 tgtcacccgg gaccaactgg cggagatggg ctgcgacctc gcccagggtt acctgatctc
     2341 ccgcccgctt caggtggacc ggttcgacag ctggttggca gcccgcaccg tctgcgttcc
     2401 tggcgcacac gacgagaccg tgctcacgct ggtgcggtga tcttggttcg gtggtcacgg
     2461 cgctgccccg tcaccgacca cgctgtcacg gcccgcgccg gtgttgtgta gagtcgtcgt
     2521 cgctcgctca ctcgagcgct gccccaatag ctcagacggc agagcgtctc catggtaagg
     2581 agaaggtcta cggttcgatt ccgtattggg gctcggtcaa ggttctgggc gaaggggctc
     2641 tggccatcgt gtcggagttc tcgaagttct gcccggaggc ggtgtagctc agtcggaaga
     2701 gcaagcggct cataatcgct gtgtcgccgg ttcgagtccg gccaccgcta ccaccggccc
     2761 ggcgcaccaa gcgctggtcg ccgcaggccc caaccgcagt ggatgacagt ttcgtgagag
     2821 agaggcaccc gtggccgcca ccgacattcg gcccaagatc acgttggcgt gcgaggagtg
     2881 caagcaccgc aactacatca ccaacaagaa caggcgcaac gatcccgacc ggctcaccat
     2941 ccgcaagtac tgcccgaact gcaacaagca ccgggcgcac cgcgagaccc gctgagccac
     3001 gtcgcctgcc cgggtagcct gcgccggtgg cacttgatcc ctccgtcgtg ggaagatctt
     3061 atcccgcagc ggatacctac caggtgggac gggagaagat ccgggagttc gccgaagcca
     3121 tcggggacga caacacggtc taccgggatc ctgacgccgc ccgatccttc ggctaccccg
     3181 atgtgatcgc cccgcccacg ttcgcgatca tcctggccct gcgagcgcag caggcgctga
     3241 tcaatgatcc cgatctcggg ctggactaca gccggatggt gcacggtgat caggcgttca
     3301 tccaccaccg gccaatcctg gccggcgacg agctggccac cacattgcat gtcgacggcg
     3361 tccggaccat gggtggcaac gatctgctca cagtgcgttg cgagatcacc gaccgcgcgg
     3421 gcgacccggt gaccaccgcc cgatcgctgc ttgtcgtccg cggcccagac aaggaaccgg
     3481 acggggagca agaccggtga cccgacgccg ccgcaccgag gtcgccgaag gggaagagct
     3541 gcccgtcctg caggtcagcg tcacccgagc cgagttgatc cgctacgccg gagcctccgg
     3601 cgatttcaac ccgatccact ggaatgaacg ggtcgcgcgt gaggtcggcc tacccgacgt
     3661 gatcgcccac ggcatgctca ccatggcgct ggccaaccgc ctggtgacgg gatgggctgg
     3721 agatcccggc gccatcgtgg agtgcggcgt acgtttcacc cgtccggtcg tggtgcccga
     3781 cgacggtgca ggagcactgg tggaactgtc cggcacggtg tcgcaacggc gtgacgacgg
     3841 caccgtcgtg gtcgccatca ctgcctgggc tggcggtcaa accgtgctcg gcaaggccac
     3901 cgccaccgtc gcactggcat aagtgtccct gttttgctgg gagg
//
LOCUS       DATADN010000360         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1239587, whole genome shotgun sequence.
ACCESSION   DATADN010000360 DATADN010000000
VERSION     DATADN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239587"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1421)
                     /locus_tag="VHH53_04725"
     CDS             complement(255..1421)
                     /locus_tag="VHH53_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX2289496.1"
                     /translation="MLLIVAVLGGCGGRVFPPDSPSPMHPVSTPDHPGTAPGVSAVSG
                     LPGMPALVDPDNVYAAAGAEMLSPAVRADRPLVYVPHTNSGDVWVIDPATFTVIARYP
                     TGRQPQHVVPSYDMRTLYVTDSRDNHLLAFDPTTGLPGQRIPVIDPYNMYFTPDGKYA
                     ISVAEARRQLVWYDPHSWIQRDVTPAPACAGINHAEFSADGHTVVFSCEFAGAVAVVD
                     VASHQLLRMIPMPTRHTHMGPQDVKLAPDGSAYYVADCDAGGLWILDRAATTVQRFIP
                     TGRCAHALYLSRDAKRLFVTNREEGSVSVLTANSATPITKWQIPGGGSPDMGNLTADG
                     TQLWLSGRYDNAVYVLSTSNGALIKKIPVGNGPHGLCVWPQPGRYSIGHTGITR"
BASE COUNT          270 a          455 c          509 g          283 t
ORIGIN      
        1 gcatagcagg aggtcaaacc tcgcgttggc atttgatcca atcttccagt tggagggccc
       61 gctcgtgctc acccagctcg cgaaaatcga cggcgaggtt actggccgac agcagagtgt
      121 gggggtggtc ctcacccacc atgcggaatt actcaccccg gctgtatact cgaccacccg
      181 catactcccc gcaactcggg atcatcggga tcgccacgtg ttcgaacggg gatcgtcgga
      241 gtcgtgtggg tggcttagcg ggtgataccg gtgtgtccta ttgagtagcg tcctggttgg
      301 ggccacacgc acagtccgtg cgggccgttt ccgaccggga tctttttgat gagggcgccg
      361 ttgctggtgg atagcacgta gacagcgttg tcatagcggc ccgagagcca taattgggtg
      421 ccgtcggcgg tgaggttgcc catgtcgggg ctgcccccgc cggggatctg ccacttggtg
      481 atcggcgtcg cgctgttggc ggtgagcacg ctgacgctgc cttcttcgcg gttggtgacg
      541 aacaatcgct tcgcgtcgcg ggataggtaa agcgcgtgcg cgcagcgacc ggtggggatg
      601 aaccgctgca ccgtggtggc cgcacggtcg agtatccata acccgccagc gtcacagtcg
      661 gccacgtagt aggccgagcc gtccggggcg agcttgacgt cttggggtcc catgtgggtg
      721 tgccgcgtcg gcatggggat catcctcaac aactggtgcg aggccacgtc gaccacggcg
      781 actgcgccgg cgaattcgca gctgaacacg acggtatgac cgtcggcaga gaattcggca
      841 tggttgatgc cggcgcatgc tggggctggc gtgacatcgc gttggatcca gctgtgcggg
      901 tcgtaccaga ccagctgccg acgagcttcg gccaccgata tcgcgtactt cccgtccggg
      961 gtgaagtaca tgttatacgg gtcgattacg gggatccgct gaccgggcag gcccgttgtc
     1021 gggtcgaaag ccagtaggtg gttgtcccgc gaatcggtga catagagggt ccgcatgtca
     1081 taggaaggca cgacgtgctg gggctggcgc cctgtcgggt agcgggcgat caccgtgaag
     1141 gtcgctggat cgattaccca cacatcacca gagttggtat gaggtacata gaccagcggc
     1201 cggtccgctc gcaccgcagg tgacagcatc tccgcaccgg cggcggcata gacattgtct
     1261 ggatcaacca atgctggcat ccccggcagt ccgctcactg cggacacccc tggtgcggtg
     1321 ccgggatgat ctggcgtgct cacgggatgc atcgggctgg gggagtccgg cgggaaaacc
     1381 cgcccaccac agccgcccag caccgcaacg atgagtagca caacgaactg cagtggctga
     1441 cgactccgca tggcttagcc cagcagctcc ccgctcgcca cctccgacaa tgaccgtccg
     1501 gccacatcga gcagtac
//
LOCUS       DATADN010000361         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_645548, whole genome shotgun sequence.
ACCESSION   DATADN010000361 DATADN010000000
VERSION     DATADN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645548"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VHH53_04730"
     CDS             <1..584
                     /locus_tag="VHH53_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2289497.1"
                     /translation="VTMPTGLALIAVDTAGETTIVVIPGANAELHVEEEDVIGADAVL
                     TVLEVPEHAVTAAARHATGLFVLNAAPARPVPPEVLQRADLVVVNSAEHAALPGLDAA
                     RAVAVTYGARGAVLFRDGIEVATAAPPPVRVVDGTAAGDAFVGAAVAGLLSGLRDADM
                     LVRACAAGALAATRSGAQPSLPTAAEIDEVLNR"
     gene            581..1507
                     /locus_tag="VHH53_04735"
     CDS             581..1507
                     /locus_tag="VHH53_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX2289498.1"
                     /translation="MSVPTIIDTDPGVDDAVALMLAAASPELQLLAVTTVFGNAGVEV
                     TTANALRLRALAGLGDLPVAAGAARPLVYPRTQRTGSWHGSDGLVGRANLLPEPLGPI
                     HQRGAVALIADLLHVAAAPVTLVAIGPLTNIALLLAAHPRVKPRIDRIVIMGGHLSGL
                     PAESNIHSDPEAARRVLVEEDVPTTLVPLDLTRRATISRAWLAELAAAGPRCATLASI
                     LEHGREHSRRATGRDVVALHDPLAVLEAAVAGTLQTTSMPLEVVCDHGPTRGAVVAAN
                     GHSVDGHAVRVAVDANLPSINAIILERLGGLG"
     gene            complement(1916..>2523)
                     /locus_tag="VHH53_04740"
     CDS             complement(1916..>2523)
                     /locus_tag="VHH53_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX2289499.1"
                     /translation="VECPLPGGSATVRNVFDLDLHSILYLTAQHPEIPAAFAAASLLL
                     SGAAWWLAPRWGWAQVPAALAGCGLALALAVTLVRPVGPFSPSGLNPLIILHECGIGS
                     LSLARTYEKLNVVMLLPFAFFATLATRRPAIIAAACLLISGLVEFVQGATGGGTCQAR
                     DLMHNTVGSVLGVGLAAVVLRLSVRSREGTAGVESPLKTLR"
BASE COUNT          408 a          879 c          858 g          378 t
ORIGIN      
        1 gggtcacgat gccgaccgga ctggcgctga tcgccgtcga caccgcgggt gagaccacca
       61 tcgtcgtgat accgggcgcg aacgcggaac tgcatgtgga agaggaggac gtgatcggcg
      121 ccgacgcggt gctcaccgtg ctggaggtcc ccgagcacgc cgtcactgcc gccgcccgac
      181 acgccaccgg acttttcgtg ctcaacgcgg ccccagcccg gccggtgcca cctgaggtgc
      241 tccagcgcgc cgatctggtc gtggtcaaca gtgctgagca cgccgcgctg ccgggtctgg
      301 acgctgcccg agccgttgcc gtgacctacg gagctcgtgg cgcagtgctg ttccgcgacg
      361 gcatcgaggt cgcaacggcg gctccgccac cggtccgcgt ggtcgacggc acggccgccg
      421 gtgatgcgtt cgtcggtgcc gccgtggcgg gcctgctcag tggactccgg gacgccgaca
      481 tgctcgtccg ggcctgcgcc gcgggagcgc tggcggccac ccgatccggc gctcaaccat
      541 ccctgcccac cgccgctgag atcgatgagg tactcaaccg ttgagtgtac cgaccatcat
      601 cgacactgac ccgggggtgg acgacgcggt ggcgctgatg ctggccgctg ccagtccaga
      661 gttgcagctc ctcgccgtga ccacggtgtt cggcaatgcc ggtgtggagg tcaccaccgc
      721 caacgcactg cggctgcggg cgctcgcggg gctcggtgac ctgccggtgg cggccggagc
      781 cgctcggcca ctggtgtatc cgcggacgca gcgaaccggt tcatggcatg gctccgacgg
      841 cctcgtgggg cgtgccaacc tgctgcccga acccctcgga ccgatccatc agcgcggcgc
      901 ggtggctctg atcgccgatc tgctgcacgt cgcagctgcc cccgtcacgc tggttgccat
      961 cggaccattg acaaatatcg cgctgttgct cgccgctcat ccccgggtga agcctcggat
     1021 cgaccgcatc gtgatcatgg gtggtcacct cagcggcctg ccagccgagt ccaatatcca
     1081 ctccgatccc gaggcagccc gccgggtgct cgtcgaggag gacgtgccaa ccacgctggt
     1141 gcccctggac ctgacccggc gcgccacgat cagccgtgcc tggctggccg agctcgctgc
     1201 cgcgggaccg cgctgcgcca cgctggcgag catcctcgag cacggtcggg agcacagccg
     1261 cagggccacc ggacgggacg tggtagcgct gcacgacccg ttggccgtgt tggaggcagc
     1321 tgttgcgggc acgttgcaaa cgacgtccat gccgctggag gtggtctgtg accacggacc
     1381 cactcgcggt gccgtggtgg ccgcaaacgg ccacagcgta gacgggcacg ccgtgcgggt
     1441 agcggtggat gcgaatctgc cctcaatcaa cgcgatcatt ctggagcgac tcggaggctt
     1501 ggggtgatgc cgctggcgct acggcgagtc cgctgtcgcg agagaccgta cgcgaccctc
     1561 accgggtcga attgatcatc tcgaccgcgt cgggacaccg gtatgcgcta tctccgaacg
     1621 ctgcgtggtc tgatcggagc tcggccagct gacgctgtgg tgacgatcgc cggccagcag
     1681 ggcacgcaac gcctcttcgt gcagccctcc aaggtcgtac caccggtagc cgcgcccctt
     1741 cgcccaccgg atgatctccc acctgatggc ggctggaacg ctgagccgac gggtacagcg
     1801 gcgcgtagcc accccgaccg cgcaggcgtg tgcacgcggt gagctgggtg acatcgctgc
     1861 ctcggtagcg tccacgaggg tctcagtgac agagggcctc gcaagtgcga ctgagctacc
     1921 tcaacgtctt gagcgggctc tcgacgccag cggtgccctc acgggatcgg accgacagcc
     1981 gtagtaccac cgcggccagc cccaccccga gcacgctgcc aacagtgttg tgcatcaagt
     2041 cacgggcctg gcaggtgccg cctccagtgg cgccttgcac gaactccacc aacccgctga
     2101 tcagcaggca ggctgcggcg atgatggccg gacggcgcgt cgccagtgtc gcgaaaaacg
     2161 caaacggcaa cagcattacg acattgagct tctcgtaggt gcgggccaac gacaggctgc
     2221 cgatcccgca ctcgtgcagg ataatcagcg ggttgagacc gctcggtgag aacggcccga
     2281 cggggcgcac caaggtgacg gcgagcgcca acgccagccc gcacccggcc agcgccgccg
     2341 gtacctgcgc ccatccccag cgcggtgcga gccaccaggc tgcaccgctg agcaacagcg
     2401 acgccgcggc gaaggcagcg ggtatctcgg ggtgctgcgc cgtcaagtaa aggatggagt
     2461 gcagatcgag gtcgaacaca ttgcgcacgg tagccgaccc gccgggaagc ggacactcga
     2521 ctc
//
LOCUS       DATADN010000362         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1104416, whole genome shotgun sequence.
ACCESSION   DATADN010000362 DATADN010000000
VERSION     DATADN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104416"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VHH53_04745"
     CDS             <1..580
                     /locus_tag="VHH53_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2289500.1"
                     /translation="WQQITAGTAYYAVLHTVGDPRRWEQLSSALAAARDGNGVQLAAL
                     VEPMLTKTQGLPARFDPALATHCNDTSTRVPPERAQQLVAQWRQRFALFGPLFAQRLL
                     LCSAWPVPSPPPIGPGDRSVPPVLVVATAGDPVTPAEGARRTAQLLPSATLVIWQGQA
                     HGALPHSSCMVDLVTRFLMDASIPREGTLCPP"
     gene            complement(725..1771)
                     /gene="ligD"
                     /locus_tag="VHH53_04750"
     CDS             complement(725..1771)
                     /gene="ligD"
                     /locus_tag="VHH53_04750"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2289501.1"
                     /translation="MGRGDALELTVGGPGGERTVRVSNPDKVYFPERAITKRQVVEYY
                     LAVSDPLLRVLAQRPVTLKRFVDGVNGQPFYAKRVPRGAPDWVDSVEITFPSGRTARE
                     VCPTEPAVLAWAANLGTFDFHPWPVRRPLVDHPDELRVDLDPQPGTGFTDAVVVAGVL
                     REVLSEAGLVGYPKTSGGRGIHVAVRIRPQWSFVHVRRAVIALAREVARRTPQRATVS
                     WWKEQRGRRVFLDFNQAARDRTIASAWSVRGTPRATVSMPMVWDDLPQVHPDDFDVLT
                     VPALLAERGDPHAGLDGAAFGLETLLEWAVRDERDHGLGDLPYPPEYPKMPGEPLRVQ
                     PSRAKRQIREDGPD"
     gene            1826..2152
                     /locus_tag="VHH53_04755"
     CDS             1826..2152
                     /locus_tag="VHH53_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289502.1"
                     /translation="MHSEPQPDRSGHQPNTQERRRLDDELIGLDRDDPEVQAFFEHLD
                     RPHRLRPGYTVEGYLAGVSDFAESANRTQGSRRAAAVLVVGLILLGVAFTLWEAATFI
                     VTVLFG"
     gene            2173..2610
                     /gene="msrB"
                     /locus_tag="VHH53_04760"
     CDS             2173..2610
                     /gene="msrB"
                     /locus_tag="VHH53_04760"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX2289503.1"
                     /translation="MKPVKGVTPKVVKSDAEWREQLSPQEYTVLRQAGTEPPGTGEYE
                     HTKTRGVYECRACGAELFRSDHKFESHCGWPSFFTPLAGDAVLLREDGSMFMKRVEVL
                     CASCHSHLGHVFDGEGYDTPTDQRYCINSISLRLVPDDGQPGS"
     gene            complement(2607..>3116)
                     /gene="hemQ"
                     /locus_tag="VHH53_04765"
     CDS             complement(2607..>3116)
                     /gene="hemQ"
                     /locus_tag="VHH53_04765"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX2289504.1"
                     /translation="RADADWMIWWHAEEIESLQAGYADLRRATALGRASVPVWSTVAL
                     HRPAEFNKSHVPAFLAGEEPRRYVCVYPFVRSYEWYLLPDTERRELLAEHGMQARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRALRASRARRHVREEVPFFTGPRVP
                     VAELVAALP"
BASE COUNT          558 a         1064 c         1023 g          471 t
ORIGIN      
        1 ctggcagcag atcaccgcgg gcacggctta ctatgcggtg ctccacacgg tcggcgaccc
       61 gcgacgctgg gagcaactgt ccagcgcact ggcggcggcc cgtgacggca acggggtaca
      121 gctcgctgcg ctcgtcgagc cgatgttgac gaagacccaa gggctaccgg cgcgctttga
      181 cccagccctg gcaacccact gcaacgacac ctcgacccgg gtaccgcccg agcgggccca
      241 gcagctggta gcgcagtggc ggcaacgttt cgcgctgttc ggtccgctgt tcgcgcaacg
      301 tctcctgctg tgcagcgcgt ggccggtgcc ctcgccgcca ccgatagggc cgggtgaccg
      361 gtcagtaccg cccgtgctcg tcgtggccac tgccggggac ccggtgaccc cagccgaagg
      421 ggccaggcgc accgcgcagt tactgccgtc ggccacgttg gtgatctggc aaggacaggc
      481 gcatggcgca ctgccgcaca gttcctgcat ggtcgatctg gtcacccgct tcctgatgga
      541 tgcctcaata cccagagaag gcacgctatg cccgccatag cgtgcacatc tgagccgagg
      601 aatgagaaac gggcccccgc cgggggaggc tcagcggggg cccgatgagg tgggagtgag
      661 gaggaatcac cacctgccca ggagatcgcc cgagaccccg agtccgttac gaactcatac
      721 tgagctaatc gggcccgtct tcacgtatct gacgtttagc tcgagatggt tgtacccgta
      781 gcggttcgcc gggcattttc ggatattccg gcgggtaggg cagatcaccg aggccgtggt
      841 cacgctcatc gcgtaccgcc cactccagca gtgtctccaa gccgaaggct gcaccgtcca
      901 gcccggcgtg cgggtccccc cgctcggcga gcaacgcggg caccgtcaac acatcgaaat
      961 cgtcgggatg cacctgtggc aaatcgtccc acaccattgg catcgacacc gtggcgcgcg
     1021 gagttccgcg taccgaccac gccgaggcga tcgtgcggtc ccgggcagcc tgattgaagt
     1081 cgaggaatac tcgccggccg cgctgctcct tccaccacga cacggtggcc cgttgcggag
     1141 tccgccgcgc cacttctcga gccagcgcga tgaccgcccg gcggacgtgc acaaaggacc
     1201 actgcgggcg gattcgaacc gcgacgtgaa tcccgcgtcc acccgaggtc ttcgggtaac
     1261 cgaccagacc tgcctctgac agcacctcgc gcaggacacc ggccaccaca accgcgtcgg
     1321 tgaagccggt gcccggctgc gggtcgagat ccacccgcag ctcatctgga tgatccacga
     1381 gtgggcgccg taccggccat ggatggaagt cgaaggtgcc caggttcgcc gcccaagcca
     1441 gcaccgccgg ctcggtggga catacctcgc gagcagtgcg gccggagggg aaggtgatct
     1501 ccaccgaatc cacccagtcc ggggcacccc ggggtacccg cttggcgtag aagggctgcc
     1561 cgttcacgcc gtccacgaag cgtttcagcg tgaccgggcg ctgcgcgagc acccgcagca
     1621 gcggatcgga gactgcgagg tagtactcca cgacctgccg cttagtgatg gcacgctcgg
     1681 ggaaatagac cttgtccggg ttggatactc gcacggtgcg ctcgccgccc gggccaccca
     1741 ccgtgagctc cagcgcgtcg ccacgaccca cgaacggcaa gctaccgcac agctatccga
     1801 caaatcggga taccgtgatc cggtggtgca cagcgaaccg cagcccgacc ggtcgggtca
     1861 ccagccgaac acccaggaac ggcgtcgact ggacgacgaa ctgatcggct tggaccgaga
     1921 cgatcccgag gtgcaggcct tcttcgagca cctcgaccgc ccccaccggt tgcgccctgg
     1981 gtacacggtc gagggttacc tggccggtgt cagcgacttc gccgagtccg ccaaccgcac
     2041 gcagggctca cggcgggcag cagccgtact ggtggtcggg ctgatcctgc tcggggtggc
     2101 cttcacactg tgggaagcgg cgacgttcat cgtcacggtc ctgttcggct gaggccgcgt
     2161 agcgtggcag ctatgaaacc ggtcaaaggc gtgacgccca aggtcgtcaa gtccgacgct
     2221 gagtggcgcg aacaactcag cccacaggaa tacaccgtac tgcgccaggc cgggaccgaa
     2281 ccgccgggaa ccggcgagta cgagcacacc aagacccgag gcgtctatga gtgccgggcc
     2341 tgcggggcgg agctgttccg cagcgaccac aagttcgagt cgcactgcgg atggccgtcg
     2401 tttttcaccc cgctggctgg tgacgcggtg ctgctgcgcg aggacggcag catgttcatg
     2461 aagcgtgttg aggtgctctg cgctagctgc cacagccatt tgggtcacgt cttcgacggc
     2521 gagggttacg acactcccac cgatcagcgg tactgcatca actcgatctc gcttcggttg
     2581 gtgcccgacg acggtcagcc cggcagttaa ggcagggcag ctaccaactc ggcgacgggc
     2641 acccgggggc cggtgaaaaa cggcacctct tcgcggacgt gccgccgggc ccggctggcc
     2701 cgcagcgcgc gcatcagatc aacgatgcgg tgcagctcgt cggcctcgaa cgcgaggatc
     2761 cactcgtagt cgcccaacgc gaacgaggcg accgtattgg ctcgcacatc gggatagtcg
     2821 cgggcctgca tgccgtgctc ggcgagcaac tcacggcgct cggtgtcagg cagcaggtac
     2881 cactcgtagg agcgcacgaa cgggtagaca cagacgtagc ggcggggctc ctcacccgcc
     2941 agaaacgccg gcacgtggct cttgttgaac tccgccgggc gatgcaatgc caccgtggac
     3001 cacaccggca cgctggcccg acccagggcg gtggcacggc gcaggtcggc gtaaccagcc
     3061 tgcagtgact cgatctcctc ggcgtgccac cagatcatcc agtcggcgtc ggcgcg
//
LOCUS       DATADN010000363         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1239736, whole genome shotgun sequence.
ACCESSION   DATADN010000363 DATADN010000000
VERSION     DATADN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239736"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /gene="cofE"
                     /locus_tag="VHH53_04770"
     CDS             complement(<1..584)
                     /gene="cofE"
                     /locus_tag="VHH53_04770"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX2289505.1"
                     /translation="MSVSDAGRMTPIRDHAAPGEVRILPVRGLPEFRPGDDLAAAVAS
                     AAAWLIDGDVVVITSKAVSKVEGRLCAVPADPLARDAARRELVTAEARQVLARRGRTL
                     ITMNALGVVQAASGVDASNVERDEVALLPVDPDASATRLRAGLAAALGVDVAVVITDT
                     MGRAWRVGQIDAAIGAAGLSVLHRYAGAVDRHGN"
     gene            complement(604..>1529)
                     /gene="cofD"
                     /locus_tag="VHH53_04775"
     CDS             complement(604..>1529)
                     /gene="cofD"
                     /locus_tag="VHH53_04775"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEX2289506.1"
                     /translation="VDAGVGTVAAEHEITAVVNTGDDIWLHGLRICPDLDTCMYTLGG
                     GIDAERGWGRAGETWAVKAELERYSADPDWFALGDRDLATHLVRTRMLRAGYPLSAVT
                     AALCHRWRPGVRLLPATDDRCETHVVVADSQDDDTQGRRALHFQEWWIRHRAALPASA
                     FVPVGADDAEPALGVCDAIAEADAVLLAPSNPVVSIGSVLAIAGIRQAVRNTPCRVIG
                     LSPIVGGRAVRGMAQACLAAIGVACDAGAVGRHYGARSEGGILDGWLVHTGDAATVPG
                     VQVREVPLLMSDVAATAVMARAALELAGLAT"
BASE COUNT          232 a          593 c          496 g          208 t
ORIGIN      
        1 tcgttgccgt gccggtcgac ggcgcctgcg tagcggtgca gcacgctgag cccggcggcc
       61 ccgatcgccg cgtcgatctg cccgactcgc cacgctcgac ccatagtgtc ggtgatcacc
      121 accgcgacgt cgacgcccag cgccgcggcc agtccggccc gcagccgcgt ggcgctggca
      181 tccggatcga cgggtagcag cgcgacctca tcgcgctcca cgttggacgc gtccactccc
      241 gatgcggcct gaaccacgcc cagcgcgttc atcgtgatca gcgtacggcc gcggcgcgcg
      301 agcacctgcc tggcctcagc agtgaccagc tcgcggcggg cggcgtctct ggccagcggg
      361 tcagccggta ccgcgcacag ccgtccctcc accttggaca ccgctttgct ggtgatgacg
      421 acgacgtcgc cgtcgatcag ccacgctgct gcgctggcca ccgcggcggc caggtcgtcg
      481 cccggccgga actcgggcag cccgcggacc ggcagaatcc gcacttcgcc cggagcggcg
      541 tggtcccgga tcggtgtcat gcggcccgcg tcggagacgc tcatgttgcc agcccggaga
      601 cgctcatgtt gccagcccgg ccaactcaag agccgccctg gccatcaccg cggttgcggc
      661 cacatccgac atcagcagcg gcacctcccg gacctgcacc ccaggcaccg ttgcggcatc
      721 cccggtgtgc accaaccagc catccaggat cccgccctcg ctgcgggccc cgtagtgcct
      781 gccgaccgca ccggcgtcgc acgccacgcc gatggcggcc aggcaggctt gcgccatccc
      841 gcgcactgcg cggcctccga cgatcgggga aagcccgatc acccggcagg gcgtgttgcg
      901 cacagcctgc cggatgcccg caatggccag cacgctgccg atcgacacga ccggattgga
      961 cggcgcgagc agcaccgcat cagcttcggc gatcgcgtcg cacaccccaa gggccggttc
     1021 cgcgtcgtcg gcacccaccg gaacgaacgc gctggccggc agtgccgcac ggtgccggat
     1081 ccaccattcc tggaagtgca atgcgcgacg gccctgggtg tcatcgtcct gggagtcggc
     1141 caccaccacg tgagtctcac aacggtcatc ggtggcaggc agcagccgca ccccaggccg
     1201 ccatcgatgg cacagcgccg cggtgaccgc agacagcgga tagccggccc gcaacatccg
     1261 ggtgcggacc aggtgagtgg ccaggtcgcg gtcgcccagc gcgaaccagt ccggatcggc
     1321 gctgtagcgc tccagttccg ctttgacggc ccaggtctcc ccggcccggc cccagccccg
     1381 ctcggcatcg atccccccgc cgagggtgta catgcaggta tccagatccg ggcagatccg
     1441 cagaccgtgt aaccagatgt cgtcgccggt gttcaccacc gcggtgatct cgtgctcggc
     1501 ggcaacggta ccgacgccgg catcgactg
//
LOCUS       DATADN010000364         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_954763, whole genome shotgun sequence.
ACCESSION   DATADN010000364 DATADN010000000
VERSION     DATADN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954763"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..264
                     /locus_tag="VHH53_04780"
     CDS             79..264
                     /locus_tag="VHH53_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289507.1"
                     /translation="MITDVRDRAALADEVLADVRDPDEVREPDADARARAAKLSMPDL
                     PASGTSIMTRHHPWRLA"
     gene            261..896
                     /locus_tag="VHH53_04785"
     CDS             261..896
                     /locus_tag="VHH53_04785"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2289508.1"
                     /translation="MIPTDNYSSRLGERVRLVVLHTTEGARDVDSLGAYFQTGTDQAS
                     YHAAFNDHRAETYVPYSAAAWSIRNGNQESDNAAFCGFSAWDRVEWLRHPRMLELGAA
                     WVADRCMARGLPIHRLSLAGTAAALRNPSHPGGVIMHRDYTYATGDGTHTDAGDGLPW
                     DVILASAHQIAAGMVAASNTSGKCSTHSAKSPARHPWAVQGSCQARRGLSA"
     gene            complement(822..1394)
                     /locus_tag="VHH53_04790"
     CDS             complement(822..1394)
                     /locus_tag="VHH53_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289509.1"
                     /translation="MDRWSSTTLLAYVFWHWPPASADASGYEADLVAFHASLACAPPA
                     GFLRSATFQVAGAPWLPQQRDCYEDWYLIRDWAAVGTLNTNAVAPAHTKAHDRVARQA
                     ECGTGGLYALRAGDGMPGGTAATWFAKPAGWSYADLESALQSCLDRGLTLWQRQMVLS
                     PAPEFCLRGEAAPGLAAPLHGPRVPCRALR"
     gene            complement(1416..2000)
                     /locus_tag="VHH53_04795"
     CDS             complement(1416..2000)
                     /locus_tag="VHH53_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEX2289510.1"
                     /translation="MRRALLIVDVQNDFCEGGSLAVPGGAEVTRAINNHIAVGPRYAH
                     VVATRDYHVDPGTHFSETPDYVDSWPPHCVAGTQGADFQPCLDTSRIEAVFSKGRHEA
                     AYSGFEGTTDDGTSLAVWLRDRNITALDIVGIATDHCVRATALDAVREGFDTRVLLNL
                     TPGVAPESTETALEQMRHAGVVLAGKPIVRSSQR"
BASE COUNT          347 a          710 c          744 g          372 t
ORIGIN      
        1 ggaccgcaga gcgcaatgac accatccagc tagtacgagc ccacccgggt tgctgcacca
       61 ccgccggaag gaagcacagt gatcacggac gtgcgcgacc gggctgcatt ggccgacgag
      121 gtgctcgctg atgtccgtga ccccgatgag gtccgtgaac ccgacgcgga cgcccgggcg
      181 cgggctgcca aactcagcat gcctgacctc ccggcatccg ggacctcgat catgacgcgg
      241 catcatcctt ggcggttggc gtgatcccca ccgataacta ctcgagccgt ctcggggaac
      301 gtgttcgcct ggtggtgctg cacaccaccg agggcgccag ggacgtcgac agcctcggcg
      361 catattttca gactggaacc gatcaggcct cgtaccatgc agcgttcaac gaccaccgcg
      421 ccgagacata cgttccctac agcgcggccg cctggtccat ccgaaatggc aaccaggagt
      481 ccgataacgc cgcgttctgc ggattctcgg cgtgggatcg cgtcgagtgg ctgcgccacc
      541 ctcggatgct ggagctcggt gccgcgtggg tcgctgatcg ctgcatggcg cgcggtctgc
      601 ccatccatcg actatctctg gcgggcaccg ccgctgcgct gcgcaatccc agccatcccg
      661 gtggtgtgat catgcaccgc gattacacct atgccaccgg cgacggtact cacaccgatg
      721 ccggagacgg tctgccgtgg gacgtcatcc tcgctagtgc ccaccagatc gccgccggga
      781 tggtcgccgc gtcgaatacg agcggtaagt gctccacgca ctcagcgaag agcccggcac
      841 ggcacccgtg ggccgtgcag gggagctgcc aggcccggcg cggcctctcc gcgtaggcag
      901 aactccggag cgggactgag taccatctgg cgttgccaca gcgtcaagcc tctatccagg
      961 caagattgga gtgcactctc gaggtcggcg tagctccacc cggcgggctt ggcgaaccag
     1021 gtcgccgcag tgccacccgg catcccgtct ccggcgcgca gggcataaag cccaccggtg
     1081 ccgcattccg cttgccgggc cacccggtcg tgagctttcg tgtgggcggg tgccactgcg
     1141 ttggtgttga gtgtgcccac cgccgcccag tctcgtatca ggtaccagtc ctcgtagcag
     1201 tctcgctgct gcggcagcca cggtgcgccc gccacctgga aggtcgcgga gcgcaggaag
     1261 ccggccggtg gggcgcaagc cagcgaggcg tgaaaggcca ccagatcggc ctcgtagcct
     1321 gacgcgtcag cggacgctgg cggccagtgc cagaacacgt acgccagcag cgtcgtcgag
     1381 gaccatcggt ccattggctc accgacgatc acaagttacc gctggctgga gcggactatc
     1441 ggtttgccgg ccagcacgac gccggcgtgg cgcatctgct cgagcgcggt ctcggtggat
     1501 tccggtgcca ccccgggggt gaggttgagc agcactcggg tgtcgaaccc ttcgcgcacg
     1561 gcgtcgagcg cggtggcccg cacgcagtgg tcggtggcga tgccgacgat gtcgagggca
     1621 gtgatgttgc ggtcgcgcag ccacacggcg agtgacgtgc catcgtcggt agtgccttcg
     1681 aaaccgctgt aggcggcctc gtgcctgccc ttgctgaaca cggcctcgat acggctggtg
     1741 tccaggcatg gctggaagtc ggcgccttgg gtgccggcca cgcagtgtgg tggccacgag
     1801 tcgacgtagt ccggggtttc ggagaagtgg gtgcctggat cgacgtggta gtcgcgagtg
     1861 gctaccacgt gggcgtaccg cggaccgacg gcgatgtggt tgttgatggc cctggttacc
     1921 tcggcgccgc cggggacggc gaggctgccg ccctcgcaga agtcattctg tacgtcgacg
     1981 atgagaagtg ccctgcgcag cggctgttgc gttgtccgcg tcgcctcagg cgccgcagag
     2041 agcgaaatcc ccttgctcat gttgggtcct cctagctgcc gccggtgaac agccgtgttg
     2101 cggtagacaa ggccgcttcg gcgtcggtga ccatttcggc gatgatgtcc gcggcgggac
     2161 ggatgtcgtg gat
//
LOCUS       DATADN010000365         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1104882, whole genome shotgun sequence.
ACCESSION   DATADN010000365 DATADN010000000
VERSION     DATADN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104882"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VHH53_04800"
     CDS             complement(<1..584)
                     /locus_tag="VHH53_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX2289511.1"
                     /translation="MAPRPTLARERAVWAEEHLLVGVDEAGRGPLAGPVVAAAVVFPI
                     GCAAVRGLRDSKILPAAVRARLAERVRRRVLAFGVGAASAHEIDRYNIRVASAIAMRR
                     AITCLIRRSSLARFPSGQGSRTAIDPGRPYRIIIDGLPLPEIGYAHDALVDGDARCQS
                     ISAAAILAKTIRDRLMHRLAQRHPGYGWDTNVGYG"
     gene            complement(589..936)
                     /gene="rplS"
                     /locus_tag="VHH53_04805"
                     /pseudo
     CDS             complement(589..936)
                     /gene="rplS"
                     /locus_tag="VHH53_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676798.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(1107..1790)
                     /gene="trmD"
                     /locus_tag="VHH53_04810"
     CDS             complement(1107..1790)
                     /gene="trmD"
                     /locus_tag="VHH53_04810"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX2289512.1"
                     /translation="MLTIDVVTLFPEVIAPYLGASIPGRAAAAGLVQYRLVQLRDFTH
                     DRHATVDDYAYGGGAGMVLKPEPFFEAVESLRPTGPIVLLSARGRRFTHDDAVRFSLG
                     EQLTLLCGHYKDVDQRVADGLATEELSIGDFVLSGGEPAALCVLDAVVRLLPGALSDH
                     ESASSDSHYDGLLSPPSYTRPPEYRGHSVPEVLRSGNHAEIAAWRRQEAERLTRERRP
                     DLWDRYEKA"
     gene            complement(1863..>2048)
                     /locus_tag="VHH53_04815"
     CDS             complement(1863..>2048)
                     /locus_tag="VHH53_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX2289513.1"
                     /translation="LADETPLVLVSAVYELPAGLMIEVQGPKREFLLPYKKEFVQQVD
                     RAGRRLVVSPPEGLLDE"
BASE COUNT          342 a          713 c          649 g          344 t
ORIGIN      
        1 ccgtagccga cgttggtgtc ccagccgtac ccgggatgcc gctgcgccag gcggtgcatc
       61 aggcggtcgc gaatggtctt ggccaggatg gcggcggcgg agatggactg gcagcgggca
      121 tcgccgtcca ccagggcgtc gtgagcgtat ccgatctccg gcaggggcag accatcgatg
      181 atgatgcggt agggccggcc gggatcgatc gcggtgcgcg acccctggcc cgacgggaat
      241 cgcgccagcg aagaccgtcg aatgagacag gtgatggccc ggcgcatcgc tatagcgctg
      301 gccactctga tgttgtagcg gtcgatttcg tgggccgagg cggccccgac gccgaaggcg
      361 agcacccgcc gccgaacccg ctccgccagc ctggcgcgca ccgcggcggg caggatcttg
      421 ctatcgcgga ggccgcgcac cgcggcgcaa ccgatcggaa acacaacggc cgcggccacc
      481 acaggtcctg caagggggcc acggccggcc tcgtctacgc cgacgaggag gtgctcctcc
      541 gcccagaccg cccgctcccg ggccagtgtg ggtcgtggag ccatcgggtc agctctcgcg
      601 cttctcccgg attcgggcgg ccttgccgct caacgcgcgg agatagtaca gcttcgcgcg
      661 gcgcacccgt caccggcgaa ccagctcgat cgaggcaatc gttgggctgt gcaccggaaa
      721 gaggcgctct acgccgacgc ctgcggagac cttccggacg gtgaaggtct cactgatgcc
      781 ggcgcctcgc cgggcgatga ccaacccttc gaatgcctga agacgttcct tgtcgccctc
      841 gcggacgcga accatgaccc ggacagtgtc tcccggatcg aatgcaggaa tgtccgtgcg
      901 caagccctca cgggccactt ctgccaatcg ttccatggtc ggatctcctt cggtccgttt
      961 gaggcaggaa aaactgcaac gattttaaat cagcggggcc gttttgtcat cccgtgttgt
     1021 catcctgagc acggcgaagg agctgaaact cgaaaccgga gcaatgccgg ccgtctggcc
     1081 ccttcgcttc gctcagggtg acagacttag gccttctcgt atcgatccca gaggtccggc
     1141 cgccgctccc gggtgagccg ctcggcctcc tgccggcgcc acgccgcgat ttcagcgtgg
     1201 ttgccggagc gcagcacctc cgggacactg tgcccgcggt actccggcgg cctggtatag
     1261 ctcggcgggc tgagcaatcc atcatagtgc gagtcgctgc tggcggactc gtggtcgctc
     1321 aacgcaccgg gcagcagccg caccaccgca tccagcacac agagcgccgc cggctcccca
     1381 cccgagagca cgaagtcgcc gatcgacagc tcctccgtag ccagaccgtc ggctaccctc
     1441 tggtccacgt ccttgtagtg tccgcagagc agggtcagtt gctcgccgag cgagaacctc
     1501 accgcatcgt catgagtgaa gcgccggcct ctcgccgaaa gcagcactat gggcccggtc
     1561 ggccgaaggc tttccaccgc ctcgaagaac ggctccggct tgagcaccat cccggcgccc
     1621 ccgccgtagg cgtagtcatc gaccgtagcg tgccgatcgt gggtgaagtc gcgcagctgc
     1681 accagccggt actgcaccag gcctgccgcc gcggctcgtc ctggtatgct cgccccgagg
     1741 tatggtgcga tcacttccgg gaagagggtg acgacatcaa tggtcagcat agctgaacgt
     1801 tggaacgggg gaacgtcgga agctcggttc tccttcgttc cctcgttccc tccttccgtc
     1861 gttcactcgt ccagcagtcc ttccggcgga ctcaccacca gccgccgccc cgcccggtcc
     1921 acctgctgca cgaattcttt cttgtacggc agcaggaact cgcgcttggg cccctgtacc
     1981 tcgatcatca atccggcagg cagctcatag accgcggata ccagcaccag cggcgtttcg
     2041 tctgccaa
//
LOCUS       DATADN010000366         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_30963, whole genome shotgun sequence.
ACCESSION   DATADN010000366 DATADN010000000
VERSION     DATADN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30963"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..727)
                     /locus_tag="VHH53_04820"
     CDS             complement(113..727)
                     /locus_tag="VHH53_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX2289514.1"
                     /translation="MRDVRICVLGDSYVVGVGDLTGQGWVGRVALAARVRGLDLTVYN
                     LGVRRDTSLDVSRRWFDEVRLRLKDGDVFGVVFAFGLNDVDVRSGRRRVPRSRTLAVL
                     GTMLDDAHGARWATFVVGPPPVADVETTRRIGDLATGMAEVCAAREVPFVDVAAALSH
                     DGLWSQEVAAADAYHPLARGYERLAALIKDPLLLWINARTELRS"
     gene            864..1163
                     /locus_tag="VHH53_04825"
     CDS             864..1163
                     /locus_tag="VHH53_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289515.1"
                     /translation="MDEGLRRVLRQRVETYLRDVERLLRGAEPGAAWPAVRARLLPLA
                     TAWRTLLAEHAPGRRGRCRHCDRTFRPLRSRRPLCSVWRTAHAHLGGGWPSDAGQ"
     gene            complement(1181..>1600)
                     /locus_tag="VHH53_04830"
     CDS             complement(1181..>1600)
                     /locus_tag="VHH53_04830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2289516.1"
                     /translation="QRVAREAEANRIQFSAPTKGTLTSGYGARWGVFHWGIDVANSIG
                     TPILAAADGVVAESGPASGFGLWVRLQHTDGTTTVYGHINRSLVKEGQKVRAGQKIAE
                     VGNRGNSTGPHLHFEVWNAAGRKINPIVWLRNNGVDF"
BASE COUNT          263 a          551 c          522 g          264 t
ORIGIN      
        1 ccactcggtc cgctgtgctg atgtgccgca cgcccgaccc ttcgagtatc gaaagctctg
       61 ctgtcccttc aagaagagat tccggatggg tcactgatac ggcgggtcgc gttcacgatc
      121 gaagttcggt tcgggcgttg atccacaaca gcaggggatc tttgatcaat gcggcgaggc
      181 gctcgtatcc gcgagccaac gggtggtagg catctgcggc cgccacctct tggctccaga
      241 gcccgtcgtg ggacagcgca gccgcgacgt cgacgaaagg gacttctcgt gccgcgcata
      301 cctcggccat gccagtcgcg aggtcaccga tgcgccgcgt cgtctcgacg tccgcgactg
      361 gcggtgggcc gaccacgaag gtcgcccatc gggcaccgtg cgcgtcgtcg agcatcgtgc
      421 ccagcacagc cagcgtccgg gaacggggaa cacgacgccg accggaccgt acatcgacgt
      481 cgttgagacc gaaggcgaac accaccccga acacgtcacc gtccttgagc cgcaggcgta
      541 cctcgtcaaa ccaccggcgt gacacgtcca gcgaggtatc gcgccgaacg cccaagttgt
      601 agacggtgag atccagcccg cggacccggg cagccaaggc aacgcggcct acccatcctt
      661 gaccagtcag atcacccacg cccacgacgt aggagtctcc caacacgcag atgcgtacat
      721 ccctcacgcc catcagcccc ggcccgctcg ctacaccaga cgaccgtcac ggccgccgct
      781 gaacagaccc tagcgcgcag tgactgtcgg agtcgccggc tagacttcga acatacgttc
      841 gattattgtg ctgggggtgc ggaatggacg agggtttgcg cagagtgctg cggcaacgtg
      901 tggagacgta tctgcgcgac gtcgagcggc tgctccgtgg ggccgagcca ggagcggcat
      961 ggccagcggt gcgggctcgc ctgctgcccc ttgccacggc ctggcggacc ctgctggcag
     1021 agcacgctcc cggtcgccgc gggcgctgcc ggcactgcga ccgaacattt cgccctttgc
     1081 gcagccggcg cccgctgtgc tcggtgtggc gcaccgccca cgcccacctg ggtggtgggt
     1141 ggccctcgga cgcgggccag tgatcacgcg agcaccgccg tcagaagtcg acgccgttgt
     1201 tgcgcagcca cacgattgga ttgatcttcc ggccagccgc gttccacacc tcgaagtgca
     1261 ggtgcggtcc ggtcgagttg cctcggtttc ccacctcggc gatcttctgg cccgctcgaa
     1321 ccttctggcc ctccttgacc agcgagcggt tgatgtgccc gtagacggtg gtcgtgccgt
     1381 cggtgtgctg caggcgcacc caaaggccga aaccgctggc gggaccggac tctgcgacca
     1441 cgccgtcggc ggcggcgagg atcggtgtcc cgatggagtt ggcgacgtcg atgccccagt
     1501 ggaaaacgcc ccacctggcg ccgtatcccg aggtcagcgt gccctttgtt ggtgcgctga
     1561 actggatccg gttggcctcg gcctcgcggg cgacgcgctg
//
LOCUS       DATADN010000367         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1405265, whole genome shotgun sequence.
ACCESSION   DATADN010000367 DATADN010000000
VERSION     DATADN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405265"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..592
                     /locus_tag="VHH53_04835"
                     /pseudo
     CDS             1..592
                     /locus_tag="VHH53_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676986.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            994..1473
                     /locus_tag="VHH53_04840"
     CDS             994..1473
                     /locus_tag="VHH53_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2289517.1"
                     /translation="MTGNAGSPHEQLGELMAIDHHSHHIEAPNATTPPPQIELVGSVP
                     TPPVPDPSEAMTIHVTLPPGSAGSPPHRHTGPAFGYVIEGEMIFELEGEPERVVRAGD
                     TFREPGGDVIHYQDGNNLADAQTRFVVTMFGVPGQPMLIPVSGEELEERRSRRAPRP"
BASE COUNT          286 a          599 c          551 g          272 t
ORIGIN      
        1 gcccgctacc cgaccgcgat cgagcagagc tacgccaccg ctcagtggat cacccgcaac
       61 ggggcatcca agggcctgga cgccagccgc atggcggtgg ccggcgaatc ggtgggcggc
      121 aacatgacgg ccgcgctggc gctgatggcc aaggaacggg gcgacgtctc cttcgtgcag
      181 caatcgatgt actacccggt caccgacgcg gcgatgaaca ccgcctcata tgagcagttc
      241 gccaccggct actacctcac ccgcaaggca atagagtggt tctgggacgc ctataccacc
      301 gatccggcac agcgcgccga gatcacggca tcgccgaatc aggccagcac cgagcaggtc
      361 actgggctgc cgcccaccta tttgtgcgtt gacgaggccg atgtgctacg cgacgaggga
      421 gaagcctacg ccgccaagct ccgctccgcg ggcgtccccg tgacaaccgt gcgctacaac
      481 ggcaccatcc acgactttca tgctgctcaa tgcattcagc gagacccgcg ccacgcgagc
      541 cgcgatcagt caggcgaccg ctttcctgcg cgccgggctc ggaactagct gagccacccg
      601 cagctcaggg caggttactt aacgtgggct gtaggcggcc cagcacgggt cacgaccgac
      661 ggcgggcgct cgccgccgcc gtggcgggtc agctgttgac gaaccgccgg acgaacgcga
      721 gcgccttgtc agcgacctca cgccagccgc tgtcgatgat cagtgcgtgc ccccggccgg
      781 ggatcttctc gatctcggtc acgccctggt tgtcctgctg cttgtaggag gcgttgacga
      841 tcgcccacgg gacggtgtgg tcgccctcgc cagagatgag cagcagcggc cccggtcggg
      901 attcttggtg tcgaccttgg cctcggtcca gggattcagg ttggccggtg cgggccgact
      961 gtcacagaag cgggagctgt ccggtcctag ctggtgaccg gtaacgccgg ctcaccacac
     1021 gagcagctgg gagagctcat ggctatcgac caccactcgc atcacatcga ggccccgaac
     1081 gcgacgacac ccccgcccca gatcgagctc gtcggctcgg tgccgacccc gccggtgccc
     1141 gatccctccg aggccatgac catccacgtc accctgcccc cgggcagcgc cggcagcccg
     1201 cctcaccggc acaccggccc ggccttcggt tacgtgatcg aaggcgagat gatcttcgag
     1261 ttggagggtg agcccgagcg tgtcgtgcgc gccggggaca ccttccggga accgggcggc
     1321 gacgtgatcc actaccagga cggcaacaac ctcgccgacg cccagacccg attcgtcgtc
     1381 accatgttcg gcgtccccgg ccagccgatg ctcatcccgg tgagtggcga ggagctcgag
     1441 gaacggcggt cccgccgcgc gccccgtccg tgaccccggg gtgagctagg cgtcgagggc
     1501 cccttgaaga ggcactcctg cgctccgctt tcggaaccgc cggcccggcg acgtcgtttc
     1561 cgtgtgtcat cttcgcgagg tgctggtgtg tcgagagggg aacaagagtg aaccactgat
     1621 ccgtgacacg accgtcatca cggcgctgtt cgcggatctg cgtcacagtt cctgggggcc
     1681 gtgttgtcct cgcttttgac ctgccgat
//
LOCUS       DATADN010000368         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_352288, whole genome shotgun sequence.
ACCESSION   DATADN010000368 DATADN010000000
VERSION     DATADN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352288"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..914)
                     /locus_tag="VHH53_04845"
     CDS             complement(144..914)
                     /locus_tag="VHH53_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2289518.1"
                     /translation="MTGLSDEQLTDLVVRVHAVCAQHFVSRGRPHALGLFRSVAMVVC
                     LLRKNGTQDFIGAVFGVSQPTVGRRWDLLRPVIGDVLAEVIPTPREVVGRGTVLVDGT
                     VCPTWDWRDVPELYSAKVGFAGMNLQVAATLDGDLVAVGPVAVHGARHDAHAFAVSGL
                     ADLLADCPTVADLGYVGVEGIDTVPFKRLPGSDLYPSQVEFNTALSKMRAAVEHAIAH
                     LKTWRMLSEEGGRYRAPIGKYNSVVKAVVGLFFFTAYE"
     gene            997..2253
                     /locus_tag="VHH53_04850"
     CDS             997..2253
                     /locus_tag="VHH53_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX2289519.1"
                     /translation="MVWDVEVAAGSRLTDLVSLGVLASSVSRDAVDEAIRVTGRGARR
                     SDGKLPPHVMVYFTMALALFAEDDYEEVATRLTETLTAWGCWDDAWSVPTSGGITQAR
                     QRLGDEPLRQLFDRVAQPVAGSLTRGAFLGNWRLMALDGFEWDVPDTKANAAEFGYAG
                     SGEGRSAFPKVRVVSVSECGSHAMVDAEIGPVSGEGCGEQSLAARLYGRLSPEWLLIA
                     DRNFYSWSGRQAAAASGAQLLWRVKAGLTLPLLRWLPDGSYLSVLVNPRIRGKARARL
                     LDAARAGCELGADEAVMVRVVEYDVPDRVGDGTGELIALITTIIDPTTAPAAELAQAY
                     HQRWEHETGNDQIKTHLRGPGKVLRSKSPDMVRAEIYGYLLTHHAISALICQAATEAD
                     IDPDRVKFLRTVRIVRRRVADPAFSP"
     gene            2273..2467
                     /locus_tag="VHH53_04855"
     CDS             2273..2467
                     /locus_tag="VHH53_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289520.1"
                     /translation="MTVLADITRPGNLNPSRRHRTYPRVVKRGRHNSYRVKKPGDTGT
                     RHDGPATIKLANPGTHRPAA"
     gene            2561..2749
                     /locus_tag="VHH53_04860"
     CDS             2561..2749
                     /locus_tag="VHH53_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289521.1"
                     /translation="MRELLIVHPESRTVELLRVVGDRLLPVSADAEGMLRSDVLGGQF
                     ATVDGRLRVIWPDGVSDV"
BASE COUNT          487 a          877 c          927 g          471 t
ORIGIN      
        1 ctggatgacc accgcgccga acgggccgcc tttgtccggc agctcctcga agacgtcggc
       61 atccaacctg cccacggccg ccaagacgga ccctaccaat gggaacgccc cgcacccacc
      121 gcgccccgag cagacccgga agcttattca taagctgtga agaagaacag cccgacaact
      181 gctttgacaa cgctgttgta cttgccgatc ggcgcgcggt agcgaccgcc ttcttcgctg
      241 agcatccgcc aagtcttgag atgagcgata gcgtgctcca cggcggcccg cattttactc
      301 agagcggtat tgaactcgac ctggctggga taaagatcgc taccggggag tcttttgaac
      361 ggcacggtgt cgatgccctc gacaccgacg taacccagat ccgcgacggt tggacaatcg
      421 gcaagaaggt cggccaggcc ggacacggcg aaggcatggg cgtcgtggcg ggcgccgtgg
      481 actgcaaccg gtccgaccgc cacgaggtcg ccgtcgaggg tcgcggcaac ctgaaggttc
      541 ataccagcaa acccgacctt ggccgagtac aactctggga cgtcacgcca gtcccacgtc
      601 ggacacacgg tcccgtcgac cagtacggtt ccccggccga cgacctcgcg tggggtcggg
      661 atgacctcgg ccaggacatc accgatcacc ggccgcagca ggtcccagcg gcgaccgacc
      721 gtgggttggc tgactccgaa caccgcgccg atgaagtcct gcgtgccgtt cttacgcaac
      781 agacacacca ccattgccac cgagcggaac aaccccagcg catggggtcg gccacgggag
      841 acgaaatgct gcgcacacac cgcatgcacc cgcaccacca gatcggtgag ttgctcatcg
      901 cttagccctg tcatagccca atgccactta gcttggcgtg atcttgttcg gcgtgtcgtc
      961 cacggtggag tgctgtcgaa gggatgatct tgtggtgtgg tgtgggatgt tgaggtggcg
     1021 gccggtagcc ggttgacgga tctggtctcg ctgggggtgt tggcctcgtc ggtttcgcgg
     1081 gatgcggtgg atgaggcgat cagggtgacg ggccgtggtg cgcggcgttc ggatgggaag
     1141 ctgccgccgc atgtgatggt gtatttcacg atggcgttgg cgctgttcgc cgaggatgac
     1201 tatgaagagg tggcaacccg gctaaccgag acgctgacag cgtggggctg ctgggacgac
     1261 gcctggagcg tgccgacctc gggtgggatc acccaggccc gccagcggtt gggtgatgag
     1321 ccgctgcggc agctgttcga ccgggtcgcc cagccggtgg ccgggtcgct gacgcggggg
     1381 gctttcctgg gaaattggcg gttgatggct ctcgacgggt tcgagtggga cgtgccggat
     1441 acgaaggcca acgccgctga gttcggctac gccggatcgg gtgagggccg ctcggcgttt
     1501 ccgaaggtgc gggtggtgtc ggtcagcgag tgcggctcgc atgcgatggt ggacgccgag
     1561 atcggcccgg tatcgggtga gggctgtggg gagcagtcct tagccgcgcg actgtatggg
     1621 cgactgtccc cggagtggct gctgatcgct gaccgtaatt tctacagctg gtcgggcagg
     1681 caggccgcgg cggcgtcggg cgcgcagctg ctgtggcggg tcaaggccgg cctgacgctg
     1741 ccgctgctgc ggtggctacc cgacggctcc tatttgtccg tgctggtcaa tccgcggatt
     1801 cgaggtaaag cccgagcccg gctgctggat gccgcccgcg ccgggtgcga gcttggcgcc
     1861 gatgaggctg tgatggtgcg ggttgtcgag tacgacgtgc ctgaccgtgt cggcgacggc
     1921 acgggtgagc tgatcgcctt gatcaccacc attatcgacc ccaccaccgc ccctgcggcc
     1981 gagctggccc aggcctacca ccaacgctgg gaacacgaga ccggcaacga ccagatcaag
     2041 acccacctac gcgggccggg caaggtgctg cggtccaaga gcccggacat ggtgcgcgcc
     2101 gagatctacg gatacctgct gacccaccac gcgatcagcg ccctgatctg ccaggcggcc
     2161 accgaggccg acatcgaccc cgaccgggtc aagttcctgc gcacggtgcg tattgtccgc
     2221 cgccgggtcg ccgacccggc cttttccccc tgaccagcat aaacgatctt tggtgacggt
     2281 actcgccgac atcacccgac ctggcaacct caaccccagc cgacgccacc gcacctatcc
     2341 gcgagtggtc aaacgcggcc gccacaactc ataccgagtc aagaaacccg gcgacaccgg
     2401 cacccgccac gatggtccgg ccacgatcaa actcgccaac cccggaacac acagacccgc
     2461 agcatgatca aattatgcta agtggcattg ggtcaggggg cggcaacgga cgtagtgtga
     2521 tgagacgtat aagaagatcg gcttttacgc cgcgctcggg gtgcgcgagc tgctgatcgt
     2581 gcacccggag agtcgcacgg tcgaattgct gcgcgtcgtc ggcgaccggc tgcttccggt
     2641 cagcgccgac gccgagggca tgctgcgttc cgacgtcctc ggcgggcagt tcgccaccgt
     2701 agacggcaga cttcgggtca tctggcctga cggcgtctcc gacgtttagt gagctactcg
     2761 at
//
LOCUS       DATADN010000369         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_352315, whole genome shotgun sequence.
ACCESSION   DATADN010000369 DATADN010000000
VERSION     DATADN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352315"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VHH53_04865"
     CDS             complement(<1..344)
                     /locus_tag="VHH53_04865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289522.1"
                     /translation="MSAGVTKPVSVVVAGGGTAGHVEPALALADAVRRAVPQARITAL
                     GTQRGLETTLVPERGYPLELIPPVPLPRKPSADLLRLPLRVRAAVRRVREVLDAVAAD
                     VVVGFGGYVALPA"
     gene            complement(341..>1709)
                     /gene="ftsW"
                     /locus_tag="VHH53_04870"
     CDS             complement(341..>1709)
                     /gene="ftsW"
                     /locus_tag="VHH53_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX2289523.1"
                     /translation="ASSRQQTYPATQWAAVRTQLTAWLARPLTSLHLVLAVFGLLTLF
                     GLVMVLSASNVQSYTQAGSSYAVFIQQLAYCGVGLVLFWIGLRMPVRLLRGTSGLFLV
                     VSVLLLLLVLSPAGARINGGQQWLRLGPLSVQPSEIAKLALTLWGAHVLITKRTMLDQ
                     WRHLLVPVVPVAIGIFGLVMLQPDLGTTITLAIVLLALLWFAGAPAELFGAIAIAAVA
                     GALLLASVASYRSARIASFLNPGADAQGAAYQARQAMFSLADGGWWGVGLGQGSAKWD
                     YLPNAHNDFIFAIIGEELGFLGCLVVLLLFGTLAYVGIRIAARNTDPWIKLVSATVTT
                     WLVGQAAINIGYVVGLLPVTGIPLPLISSGGTSVSLTMLSFGLLANFARHEPQAVCAL
                     RSQTSRSGSQSAISRLLRLRLPVPYISAPYKSAPYKSAPYTPAPPRPRVPHPARSGGR
                     PGSRR"
BASE COUNT          319 a          577 c          594 g          219 t
ORIGIN      
        1 gcgggcaacg cgacgtagcc accgaaacca accaccacgt ccgcggcgac cgcgtcgagc
       61 acctcgcgga cccggcgcac cgcggcacga acccgcaacg gcagccgcag caggtccgcg
      121 ctgggtttac gcggcagtgg caccgggggg atgagctcca acgggtagcc ccgctcgggc
      181 accaaggtgg tctccaggcc gcgctgggtg cccagcgcag tgatccgggc ctgcggtacc
      241 gcgcggcgta ccgcgtcggc caacgccagc gctggttcga cgtgccctgc ggtgccgccc
      301 ccggcgacca cgacggatac cggcttggtc acgcccgcgc tcatcgtctg ctcccgggtc
      361 tgccgccgct tcgagctgga tgcggcacgc gaggtcgcgg cggtgccggg gtgtaaggcg
      421 ctgacttgta gggtgctgac ttgtagggcg ctgagatgta ggggacgggc aggcgcagcc
      481 gcaacagccg cgagatggca ctctgactcc cggaacgaga ggtctgcgag cgcagtgcgc
      541 agaccgcctg cggttcatgc cgggcgaagt tcgccagcag gccgaagctc agcatcgtga
      601 gtgagacgga ggtccccccc gaggagatca acggcaacgg gatcccggtg accggcagca
      661 ggcccaccac gtacccgatg ttgatcgcag cctggccgac cagccaggtg gtcacggtgg
      721 ccgacaccag cttgatccac ggatcagtgt tgcgcgccgc gatccggatg cccacgtaag
      781 ccaaggtgcc gaacagcagc agcaccacca ggcacccgag gaagcccagt tcctcgccga
      841 tgatcgcgaa gatgaagtcg ttgtgcgcgt tgggcaggta atcccatttg gcgctgccct
      901 gcccgagccc cactccccac cagccgccgt cggccaggga gaacattgcc tggcgagcct
      961 ggtacgccgc tccctgagcg tcagcgcccg ggttcaggaa ggacgcgatc ctggccgatc
     1021 ggtagctggc gacgctggcc aggaggagcg caccggcgac cgcggcgatc gcgatcgcgc
     1081 cgaacagctc agccggcgca ccggcgaacc acagcagggc gagcagcacg atggccagcg
     1141 tgatggtggt gcccagatcg ggttgcagca tcaccagtcc gaagatccca atggccactg
     1201 gaaccaccgg caccagcagg tggcgccact ggtcgagcat cgttcgcttg gtgatcagca
     1261 cgtgcgcgcc ccagagcgtc aaggcgagct tggcgatttc agagggttgc acggatagtg
     1321 ggcccagccg cagccattgc tgcccaccgt tgatcctggc gccggccggg ctgagcacca
     1381 gcagcagcag caggaccgac accaccagga acagcccact ggtgccccgc agcagccgca
     1441 ccggcatccg cagcccgatc cagaacaaca caagcccgac gccgcagtac gccagttgct
     1501 ggatgaatac cgcataggag gacccggctt gggtgtagga ctgcacgttg gacgccgaca
     1561 ggaccatcac caagccgaaa agggtcagca ggccgaaaac ggcgagcacc aggtgcagtg
     1621 aggtgagcgg gcgggccagc caggcggtga gctgggtgcg gaccgctgcc cactgagtgg
     1681 cggggtaggt ctgctggcgc gaagatgcc
//
LOCUS       DATADN010000370         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_186564, whole genome shotgun sequence.
ACCESSION   DATADN010000370 DATADN010000000
VERSION     DATADN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186564"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1081
                     /locus_tag="VHH53_04875"
     CDS             188..1081
                     /locus_tag="VHH53_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEX2289524.1"
                     /translation="MSTDLNTLLTALYVWIDDHLTGHVRAGRRPRLTDSELLTLAVAQ
                     VLLGVRSEARWLRLVPVALPGAFPYLPGQSGYNKRLRAALPLLKRVIRVLAADTDLWA
                     DPVWLVDSTPVECARSRPTVARSDLAGIAGYSYCPSHSRWFWGLRLHLVCTPAGLPIT
                     WALADPKLDERQVLMCLLDVDADLLTDRPGLLLLADKGYVSAELDDYLRARGAELLRP
                     SYRNRPPRPGQQLLAPIRQLIESVYDTLKGQLDLELHGGRSLTGVTTRVAQRLLALTA
                     AIWHNRATGQPVTRSLIAYDH"
     gene            1139..>1502
                     /locus_tag="VHH53_04880"
     CDS             1139..>1502
                     /locus_tag="VHH53_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2289525.1"
                     /translation="MCVLDADADLLAERPWLLLIADKGYVSAELDGYLRDRGAQLLRP
                     SYRNRQPRPGQRLLAPIRQLIESVHDTLKGQLDLELHSARSLAGVTARVAQRLLALTA
                     AIWHNRTNGQPVTRSLIAY"
BASE COUNT          216 a          590 c          463 g          233 t
ORIGIN      
        1 agggaccaaa ggtcgccgtg ctgctcggcg gcggcgtgtc gagaatcgcc ccgtcctacg
       61 tgcgagcagc cgacggctcc caagtcagcg tccggcgaca actcacccca gctagacgag
      121 taagtcctat cggcccgcgg ggtcgaaggc cgctgtagag gcggcggagg atgttcaggc
      181 tcgggttgtg agcaccgacc taaacaccct cctgaccgca ctgtatgtct ggatcgacga
      241 ccatctgacc ggtcacgtgc gcgccggacg ccgcccgcgg ttgaccgact ccgagctgct
      301 caccctggcc gtggcgcagg tgctgctcgg cgtgcgctcg gaggcccgct ggctgcggct
      361 ggtgccggtc gcgctgcccg gcgccttccc gtacctgccc ggtcagtccg gctacaacaa
      421 gcggctgcgc gcagccctgc cgctgctcaa gcgggtcatc cgggtgctgg ccgccgatac
      481 cgacctgtgg gccgatccgg tctggctggt cgactccacc ccggtcgagt gcgcccgctc
      541 ccggcccacc gtggcccgct cggacctggc cggcatcgcc ggctacagct actgcccgtc
      601 acactcgcgc tggttctggg ggctgcggct gcacctggtc tgcaccccgg ccggactgcc
      661 gatcacctgg gcgctggccg atcccaagct cgacgaacgc caggtgctca tgtgcctgct
      721 cgacgtcgac gccgacctgc tcaccgaccg gccgggcctg ctgctgctcg cggacaaggg
      781 ctatgtctcc gccgagctcg atgactacct gcgcgctcgc ggcgccgagc tgctgcgccc
      841 ctcctaccgc aaccgcccgc cccgccccgg ccagcagctg ctcgccccga tccggcagtt
      901 gatcgagtcg gtctacgaca ccctcaaagg ccaactcgac ctggaactgc acggcggacg
      961 cagcctcacc ggcgtgacca cccgggtcgc ccaacgactc ctggccctca ccgccgccat
     1021 ctggcacaac cgcgccaccg gccagccggt cacccggtca ttgatcgcct acgaccactg
     1081 accgggttcg gacttactcg tctaggccct gttcgataag tcgcggagat agatgttgat
     1141 gtgcgtgctc gacgccgatg ccgacctgct cgccgagcgc ccctggctgc tgctcatcgc
     1201 ggacaagggc tacgtctccg ccgaactgga cggctacctg cgcgaccgcg gcgcccagct
     1261 gctgcgcccg tcctaccgca accgccagcc ccgcccgggt cagcggctgc tcgcgccgat
     1321 ccgtcagctc atcgagtcgg tccacgacac cctcaagggc cagctcgacc tggaactcca
     1381 cagcgcacgc agcctggccg gagtgaccgc tcgcgttgcc cagcggctgc tcgccctcac
     1441 ggccgccatc tggcacaacc ggactaacgg ccagcccgtc accaggtcct tgatcgccta
     1501 cg
//
LOCUS       DATADN010000371         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_352355, whole genome shotgun sequence.
ACCESSION   DATADN010000371 DATADN010000000
VERSION     DATADN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352355"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..369)
                     /locus_tag="VHH53_04885"
     CDS             complement(148..369)
                     /locus_tag="VHH53_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289526.1"
                     /translation="MPVLNELSAIQADAIRATAAAHDRDPDELALELMHLLAADTTPE
                     ATDATPAVPALTTDHDDTVLILLPLKKLQ"
     gene            complement(498..680)
                     /locus_tag="VHH53_04890"
     CDS             complement(498..680)
                     /locus_tag="VHH53_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289527.1"
                     /translation="MSTKPGARDGAVPPEAAADSAGNADASPPALPEQIYEQATARLA
                     GIIDEWHRARDALLRG"
BASE COUNT          226 a          540 c          553 g          276 t
ORIGIN      
        1 ccggaccaca tgatcatcga ccgggccgag ccgttactgg gaatgcctca gtgacacacc
       61 tcgcgcgccg gcgacctgcc gcttcagcgc cgcagaacgg cccccaccgc caatccattg
      121 agaaaacctc agtgttgcga gcgttggtca ctgaagtttc ttcaagggca ggagaatcag
      181 cacggtgtcg tcgtggtcgg tcgtgagcgc ggggactgcg ggagtagcgt cggtggcctc
      241 aggtgttgtg tccgcggcca acaggtgcat cagctcaagc gccagctcgt cagggtcacg
      301 gtcatgtgct gcggccgtgg cacggatagc gtcggcttga atcgccgaca gctcgttcag
      361 gacgggcaaa ggcttcttcc atttgtgagc atgtgccagc actgcccaag cgctcaatgg
      421 tcgcgctcag cggtcactcg gggaccgacg ccagtgtcgg tgagtgggaa aaaacgcggt
      481 cgagtcgtcc tcgccggcta tccgcggagg agggcatcgc gcgccctgtg ccactcgtca
      541 atgatgcccg cgaggcgggc cgtggcttgc tcgtagatct gctcgggtaa cgccggcggg
      601 gaagcatcag cgttgccggc ggagtcggca gccgcctcgg gcggcaccgc cccgtcccgg
      661 gcgcccggct tggtgctcat gctccgcatg ttggctgtcg ccgcagccct gcgatagcag
      721 tgcagtcgcc gagccgctcg accgaggtgc tccccggccg ggcttcacgc gccgcccatc
      781 ctcccgtgcc cacggtcacc aacaccggcc cggcagcgcc cggtgtaggg ggatgctggt
      841 gccctcgatg ggcggtgcgg attccaacgc gacgcgggtg agtccatcga gaccgaccgg
      901 caccagccat acctcaacgg cgtcaccgcg cccatcgacc ccggggttaa ccgggcgggc
      961 gctgtcccac tccagccagg tcggctgctt ggaccgtcgt tcacccccgg cggttgcccg
     1021 gtcagcgcca gcaggcgacc gcagggggga gccggcggag acccccggag accggctgct
     1081 gagacggtga tggcagactg gtcgccaatg ttccacgctg cgtggtcgag gaccgcttcc
     1141 gccttggcgt tcccgccgga gggctgcgcg atggtgggaa cccagcggcg cccgcctgcg
     1201 gccgtggcgg tcttccccgt cggtccgttc cgaccggccg gtactcggtc ggcgtcgatg
     1261 cccgggtcga cgccgcggtc ctcgacgtct gccggtgcgc tggctgcggc cggtgctcca
     1321 tccatcctgg cgtgagctga ggagcttgtc gtgggcgatc cggcctcttc cgtcgtcgcc
     1381 ggagcggagc tgtccgccgt gtccgcgtac gacctcatgt gtaggtccgc gtacgacctg
     1441 acgtgtcgtt cctccggcgg gttgttgtcc tcgccatctg ggtggtgatc acccttcggc
     1501 tgtcagccgc caagcagaca gaccgaaggg tgaccgccca tgatcctgcc gccggaggca
     1561 tggatggatc tacgacggtt caagccgttg cgtga
//
LOCUS       DATADN010000372         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_790028, whole genome shotgun sequence.
ACCESSION   DATADN010000372 DATADN010000000
VERSION     DATADN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790028"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..634)
                     /gene="rdgB"
                     /locus_tag="VHH53_04895"
     CDS             complement(5..634)
                     /gene="rdgB"
                     /locus_tag="VHH53_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX2289528.1"
                     /translation="MSPVRLLLATRNAKKLVELRRILVSSAITGVQVIGLDDVPPFPE
                     APETAATFEENALAKARDAAAATGLPAVADDSGLEVDALNGMPGVLSARWAGRHGDDA
                     ANLQLVLDQLADIPEGRRGAAFVCAAALVVPSGTQIFQTVVRGQWRGTLSCAARGSHG
                     FGYDPIFIPEGGQRTSAELDPAEKDAASHRGQALRALLPAVRQLLDAQR"
     gene            complement(631..1383)
                     /gene="rph"
                     /locus_tag="VHH53_04900"
     CDS             complement(631..1383)
                     /gene="rph"
                     /locus_tag="VHH53_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX2289529.1"
                     /translation="MARADGRKDEALREVRITRGYQQWPAGSVLIEFGRTRVLCAASV
                     VEGVPRWRKGSGQGWLTAEYAMLPSSTHTRSDRESVRGRLGGRTHEISRLIGRALRSC
                     VDLTALGENTISLDCDVIQADGGTRTAAITGSYVALADAVTWLRAAGRLRNPRPLTCS
                     VAAVSVGVVDGRVLLDLPYEEDSRAEVDMNVVATDTGTLVELQGTGEGGATFARSTLD
                     QMLDLALKGCHELTGCQTTALAQPYPGELPRS"
     gene            complement(1435..>1784)
                     /locus_tag="VHH53_04905"
     CDS             complement(1435..>1784)
                     /locus_tag="VHH53_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2289530.1"
                     /translation="ARTAHPVECHAVRLTAAGRSLVYTGDTGPSDAVEELARGADVLL
                     AEASWPDRPDNPRGIHLSGRDAGEAATAAGVHRLLITHVPPWTEAEQVLSEAKAAFDG
                     PTELVHAGAHYEI"
BASE COUNT          270 a          647 c          601 g          266 t
ORIGIN      
        1 agcgtcagcg ctgcgcgtca agaagttgac gcaccgccgg cagcagcgcg cgcagcgcct
       61 ggccccggtg cgaggcggca tccttctctg ctgggtccag ctcggcggag gttcgctgac
      121 caccctccgg aatgaagatc gggtcgtagc cgaagccatg actaccccgc gctgcacagc
      181 tcagcgtgcc gcgccactgt cctcggacca cggtctgaaa aatctgggta cctgacggca
      241 caacaagcgc cgcggcacag acaaacgccg caccacgacg accttccggg atgtcggcca
      301 gctgatcgag caccagctgc aggttcgcgg catcatctcc gtgtcggccg gcccacctgg
      361 ccgatagcac ccccggcatg ccgttgagcg cgtccacttc caggccggag tcgtcggcga
      421 cagcgggcag gccggtggcc gccgccgcgt cccgggcctt ggccagagca ttttcctcga
      481 aggtggctgc ggtctcgggg gcttcgggaa acggcggcac gtcgtcgagc ccgattacct
      541 gcactccggt gatggccgac gagaccagga tccgccgcag ctcgacgagc ttcttggcgt
      601 ttcgcgtagc cagcagcagc cgaactgggc tcacgaccgg ggcagttcgc cggggtaggg
      661 ctgggccagc gcagtggtct ggcacccggt cagctcgtgg cagcccttca acgccaggtc
      721 cagcatctgg tccagggtgg accgcgcgaa ggtggcgccg ccctccccgg tgccttgcag
      781 ctccaccagc gtgccggtat cggtggccac cacgttcatg tcgacctcgg cgcgggaatc
      841 ctcctcgtaa ggcaagtcca gcaggacccg accatccacc accccgacgc ttaccgcggc
      901 gaccgaacag gtcagcggtc gcgggttgcg cagccgacct gccgcgcgca gccaggtcac
      961 cgcgtcggct agcgccacat agctaccggt gatcgccgcg gtgcgagttc caccgtcggc
     1021 ctggatgaca tcgcagtcca gcgagatggt gttctccccc agggcggtga ggtcgacgca
     1081 ggaccgcaga gcacgtccaa tcagcctgct gatctcgtgg gtacgcccgc cgaggcggcc
     1141 acggactgac tcgcggtcgc tgcgggtgtg cgttgaggat ggcagcatcg cgtactccgc
     1201 ggtgagccag ccctgcccgg agcccttgcg ccagcgcggt acgccttcca ccacgctggc
     1261 cgcacagagc acccgggtgc gaccgaactc gatcaaaacc gatcccgcgg gccactgctg
     1321 gtagccgcga gtgatgcgca cctcgcgcag cgcctcgtcc ttcctgccgt ccgctcgtgc
     1381 caccgggcca gcctacccag ggctggtccg ccagattccc ccgtggcagc ttggtcagat
     1441 ctcgtagtgg gcgccagcat ggacgagctc ggtgggcccg tcgaaggcag ctttggcctc
     1501 gctgagcacc tgctcggctt cggtccacgg gggcacatga gtgatcaaca gccggtggac
     1561 cccagccgcc gtcgcggcct ccccggcgtc ccggccagac aggtgtatcc cgcgcgggtt
     1621 gtccggccta tccggccagg acgcctcggc gagcagcacg tccgcgccgc gggccagctc
     1681 ctcgacggcg tccgagggac cggtgtcgcc ggtgtagacc agggacctgc cggccgcggt
     1741 cagccgcacc gcgtggcact cgacggggtg cgcggtgcgc gccg
//
LOCUS       DATADN010000373         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_352821, whole genome shotgun sequence.
ACCESSION   DATADN010000373 DATADN010000000
VERSION     DATADN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352821"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..475)
                     /locus_tag="VHH53_04910"
                     /pseudo
     CDS             complement(1..475)
                     /locus_tag="VHH53_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(532..960)
                     /locus_tag="VHH53_04915"
     CDS             complement(532..960)
                     /locus_tag="VHH53_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2289531.1"
                     /translation="MACRAGGVAPAGVTEVHTAASATAIPTIRMVAADLAARADFDLD
                     SVDELRMAVDEACTLLVRIAARNARLSSTFTVRPDRVEVAVEVDVDDVGDPLTPGSIG
                     WRVLNCLADEVNALVLPAKPGQHGLVCILLAKDAATTQRP"
     gene            complement(1108..1290)
                     /locus_tag="VHH53_04920"
     CDS             complement(1108..1290)
                     /locus_tag="VHH53_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289532.1"
                     /translation="MTLLVRMLPGQLVDDFHAQAHRIAAGMNVPMVRVTPCGHGLIKV
                     ALLDHEPLSAVLALSA"
     gene            complement(1287..>1602)
                     /locus_tag="VHH53_04925"
     CDS             complement(1287..>1602)
                     /locus_tag="VHH53_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289533.1"
                     /translation="LSTTSAPICIETEEEITMTLISSGVSTRPVDQHTELWGRRRSWW
                     TPRWLQRKREVDELARWASEVTLLWNDTMDGTDLAHHTVTAARLPFLLAPQVQSVDPG
                     HR"
BASE COUNT          301 a          481 c          518 g          302 t
ORIGIN      
        1 gaaccgtcga ggatctcatc caaagatgag ctttggtatg cctcgccggc ttgtaatcct
       61 tcgatcacct ccgaggccgg tactcccaac tgagcagcga tctcgctggg tcgaggagcg
      121 cggccgagct gctgggacag ctccgtctga gtgctcctga tggcaacatg caggtctttc
      181 agtcggcgcg gaacccgcgt tgaccagctg tagtcgcgga agtaccggcg tagttcgccg
      241 gtgatagtcg gtatcgcgca ggaaaagaaa tcgagccgcg ggtgggttcg aagcggtcca
      301 cggcattgat cagtcctacc gtggcgacct ggaccaggtc ctctagcggc tcacggcggc
      361 gggcgaagcg gcgggcgatg tgtttggcca ctggcagata gccgctgatc agttgttcgc
      421 gcagctgctg ccgctcgggg tccttcacag caaaatcggc atagcggtgg tgcagaccaa
      481 ctaagtgcgc gtactgaccg tgttcggtgt cgaccgacga ctcgcagggt gtcacggccg
      541 ttgcgtggtc gcggcgtctt tggccaagag gatgcatacc aggccatgct ggccgggttt
      601 agcaggcagg acaagggcgt taacctcgtc ggcgaggcag ttcagaaccc gccagccgat
      661 tgacccaggg gttaatggat cgcctacgtc gtcgacatcg acttcgacgg ctacctcaac
      721 ccgatcgggt cggacggtga acgtggagct tagcctcgcg tttcgggcgg cgatgcggac
      781 aagcaaggtg caggcctcat cgacggccat ccgcagttcg tcgaccgagt caaggtcgaa
      841 gtcggcgcga gcggccagat cggccgcgac catgcgaatc gtggggatcg cggtggcact
      901 ggctgcggta tgaacttcgg tgacgccggc tggtgcaaca cccccggctc tgcatgccat
      961 cgtcatctcc ttccggacat ctccttccgg atatggcaat tagcctcggt gcacccaaag
     1021 tcagttcccg atgttggtgt cagtcaaacc ccgtcaccag cagatgcggc aagcagtaag
     1081 gcaaaaaccg tcgtcaggtc acgatggtta tgccgacaag gccaggactg cgctcagtgg
     1141 ctcatgatcc agcaaagcca ccttgatcag gccgtgaccg cagggcgtga cgcggaccat
     1201 gggtacgttc atacccgcgg cgatgcggtg ggcttgggcg tggaagtcgt ccacgagctg
     1261 gccgggcagc atgcgcacca gtagcgtcac cggtggcccg gatccaccga ctgcacctgc
     1321 ggcgccaaca ggaagggtaa tcgggcagcg gtcacggtgt gatgggcaag gtcggtaccg
     1381 tccatcgtgt cgttccacag caaggtcacc tcgctggccc accgcgccag ttcgtcaacc
     1441 tctcgcttgc gctgcagcca gcgcggtgtc caccaagaac gacgacgacc ccacagttcg
     1501 gtgtgctgat cgacgggcct ggtactcaca ccgctcgaaa tgagcgtcat ggtgatttcc
     1561 tcctctgtct cgatgcagat gggtgcgcta gtcgtgctca ac
//
LOCUS       DATADN010000374         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_790140, whole genome shotgun sequence.
ACCESSION   DATADN010000374 DATADN010000000
VERSION     DATADN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790140"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VHH53_04930"
     CDS             complement(<1..1131)
                     /locus_tag="VHH53_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2289534.1"
                     /translation="MLVVGGGLAGLSAAVFLAHHGVPALLVEQHRTTSIHPKARGQSP
                     QTMEALRIAGVADQARAAGPADGARLGITIAESLVGSTLHTIVEDIPDLAHLSPAGFA
                     MASQERMEPILAERATELGARLRFSTRLQSITQDNHGVTATLRDAITGQVATARADYL
                     IAADGHRSPIRSALGIGTHGRGSLNQYMMWVFEADLAHLIDERTVTVYYLQNPALHGG
                     NGALVTTDHSGRYAIAVEYHPEHGDSAADFTAERAVEQIRAGVGIPDLPVRILHSDET
                     EFAALVADRFSLGRVHLIGDAAHVMPPTGGQGGNAAIMDGFYLAWKLAMVVTGTAGPG
                     LLDSHDAERRPVTELLVEQQYANLVQRMAPHLANGSEAAPIDS"
     gene            1247..1951
                     /locus_tag="VHH53_04935"
     CDS             1247..1951
                     /locus_tag="VHH53_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2289535.1"
                     /translation="MAVPTPPWQRPARPVQDRPPLSQRRIVDQALVLLAEEGLDGVSM
                     RRVAQALGTGPASLYAHLHNKEELCQLMLDQLIGEVAVPEPDPQHWREQLKELMRSMV
                     RAMTDHPGSAHLAIATRVPTGPNALRIMEGMLSILRSGGLSDRVTAFAGDLIGTYVSA
                     LALEAGTWAGNAPEVAAIAERTQQIRDYFAALPTDSFPHLVALAHPLTEGSVEERFEF
                     GLEVLVAGLERLSYPD"
     gene            complement(1976..>2494)
                     /locus_tag="VHH53_04940"
     CDS             complement(1976..>2494)
                     /locus_tag="VHH53_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289536.1"
                     /translation="RGTALKPHLGSGGPVLIYPEGRISADPGLWPDRGQTGLARLALA
                     TELPVLPLSQWGAHEVLPAATPPVRSLSALIGLTRIWLGTVRLRHRLHFRVHIGPPVD
                     LTGLHPGRPGDAMRAHARIMLAITAGLAPLRADQPSTPHHGDPTRPVGRPSPWRPPDE
                     TPASPGHARHTE"
BASE COUNT          390 a          797 c          903 g          404 t
ORIGIN      
        1 agagtcgata ggtgctgcct cgctgccgtt ggcgaggtgg ggcgccatcc gctggacgag
       61 attggcgtat tgctgttcta cgagtagctc agtgacgggg cggcgctcgg cgtcatggct
      121 atccagtagc ccgggaccgg ccgtacccgt gaccaccatg gcgagtttcc aggcgaggta
      181 gaagccgtcc atgatggctg cgttgccgcc ctgcccaccg gtgggaggca tgacgtgggc
      241 ggcgtcaccg atgaggtgga cgcggccgag gctgaaccgg tcggcgacga gggcggcgaa
      301 ctcggtctcg tccgagtgca ggatccgcac cggcagatct gggatgccga ctccggcgcg
      361 gatctgttcg acggcgcgct cggcggtgaa gtcggcggca ctgtcgccgt gttctgggtg
      421 gtactcgacg gcgatggcgt agcgtcccga atggtccgtg gtgacgagtg caccgttacc
      481 accatgcagg gccggattct gtaggtagta cacggtgacg gtgcgctcgt cgatgaggtg
      541 tgccaagtcg gcctcaaaga cccacatcat gtactggttg agggatcccc gcccgtgcgt
      601 gccgatgccc agggccgacc gaatggggct gcggtggcca tccgccgcga tcaggtagtc
      661 ggcgcgggca gtcgcgacct gtccggtgat ggcgtcgcgc agggtagcgg tgacgccatg
      721 gttgtcttgg gtgatcgact gcagccgggt ggagaaccgg agccgggcgc caagctcggt
      781 tgccctctcg gccaaaattg gttccatccg ctcctggctg gccatggcga agccggcggg
      841 tgagagatgg gcgaggtccg ggatgtcctc gacgatcgtg tgcagcgtcg acccgacgag
      901 gctctcggcg atggtgatgc ctagccgcgc gccgtcggct ggcccggcgg ctctggcctg
      961 gtcggcgaca ccggcgatcc gcagcgcctc catggtttgg ggtgactggc cccgcgcctt
     1021 cgggtgaatc gatgtggtgc gatgttgctc cacgagcagg gcgggcacgc cgtggtgggc
     1081 gaggaaaacg gccgccgaca agcccgccag gccgccaccg acgactagca cggggacatg
     1141 ctgcacagat acgaccactc ctggacgaac agcgttcgtt gagacgaaca acattcggct
     1201 gaagagcgac gttcgtgtca agtgcagtcc ccgacggagg taactgatgg ccgtcccgac
     1261 gccgccctgg cagcgcccgg cccgaccggt ccaggaccgc ccgccgctga gccagcggcg
     1321 catcgtcgac caggcgctcg tcctgcttgc cgaggaaggg ctcgacggcg tgagcatgcg
     1381 gagggtcgcg caagccctcg gtaccgggcc ggcttcgttg tatgcacacc tgcacaacaa
     1441 ggaagagctg tgtcaactca tgctggacca gctcatcggc gaagtcgccg ttcccgagcc
     1501 ggatccacag cactggaggg agcagctcaa ggagctgatg cgctcgatgg tccgggcgat
     1561 gaccgatcat cccggcagcg cccatctggc catcgcgacc agggtcccga ccgggcccaa
     1621 cgcgctccgc atcatggagg ggatgttgtc catcctgcgt tcgggtgggc tgtccgatcg
     1681 ggtcacggca ttcgcgggcg acctcatcgg gacctacgtc tcggcattgg cgctggaggc
     1741 gggcacctgg gcgggcaatg ctcctgaagt cgccgcgata gccgagcgga cgcaacagat
     1801 ccgcgactac ttcgcggcac tgcccaccga cagcttcccc cacctcgtcg cgctcgccca
     1861 cccgctcact gagggcagcg tcgaggaacg ctttgagttc gggctcgagg tactcgtggc
     1921 tgggttggaa cggctctcgt acccggactg aacccgtcca acgcaggcga catgatcatt
     1981 cagtgtgccg ggcgtgaccg ggcgacgctg gggtctcatc cggtggacgc cacgggctgg
     2041 gtcggcccac gggacgagtc gggtcaccgt ggtgcggcgt gctgggttgg tcggcccgca
     2101 gcggcgccag ccctgcggtg atcgccagca tgatcctggc gtgcgcccgc atcgcatcgc
     2161 cgggtcgccc agggtgcagc ccggtcaagt cgaccggagg gccgatgtgc acccggaagt
     2221 gaagccggtg ccggaggcgc acggtaccca gccagatcct ggtcaacccg atcagagccg
     2281 agagactgcg caccggtggg gtggctgccg gcagcacctc gtgcgctccc cattgggaca
     2341 gcggcagcac cggcagttcg gtcgccaggg cgagccgggc aagcccggtc tggccgcgat
     2401 ctggccacaa gccgggatcg gcgctgatcc ggccttcggg gtagatcagt accggtccgc
     2461 cggagccgag gtgcggcttc agtgccgtcc cccg
//
LOCUS       DATADN010000375         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_353111, whole genome shotgun sequence.
ACCESSION   DATADN010000375 DATADN010000000
VERSION     DATADN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353111"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..821)
                     /locus_tag="VHH53_04945"
     CDS             complement(57..821)
                     /locus_tag="VHH53_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX2289537.1"
                     /translation="MQDLVYAVYERRLRRQLGTANRPRHVAVIVDGNRRWAREVGFAD
                     VSDGHRVGAAKLVELLGWCREAGVEIATFWLLSPDNLRNRPPDQVEPLLKIIVDVVDE
                     LCAPDNPWRVRVVGALDLLPPEMAQRLTTAAAQTRERCGLAVNVAVGYGGRQEIADAL
                     RKLLLKHAEAGTTMEELAEVLDVEQIAEHLYTSGQPDPDLVIRTSGEQRLSGFLLWQS
                     AHSEFWFCDAYWPAFRKVDFLRALRDYAARHRRFGS"
     gene            complement(840..1367)
                     /locus_tag="VHH53_04950"
     CDS             complement(840..1367)
                     /locus_tag="VHH53_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX2289538.1"
                     /translation="MSDHSVDHDAHQHTTHGTAPLTAQGRRELNRLAVVATAHCLTGC
                     AIGEVLGLVIGTALGWGNGATIMLAIALAFLFGYSFTIVPVLRSGLALSAAIGVALVA
                     DTVSITVMEIVDNAIILIIPGAMEAGLDSWLFWGALAVALGVAFVVTVPVNRALIRRG
                     KGHAVVHQYHQNGRA"
BASE COUNT          302 a          528 c          503 g          248 t
ORIGIN      
        1 gtgctgacga taggcgcctc ggaagtggca ttaggtgcca taacactgtt tcccactcac
       61 gagccgaaac ggcgatgccg ggcggcgtag tcgcgcagcg cacgcaggaa gtccaccttt
      121 cgaaaggctg gccagtaggc gtcgcagaac cagaactccg aatgcgccga ctgccacagc
      181 aggaaacccg acagccgctg ctccccggag gtacggatga caagatcagg gtcgggctgg
      241 ccagaggtgt agagatgctc ggcgatttgc tcgacgtcga gcacctcggc gagttcctcc
      301 atggtggttc cggcctcggc atgcttgagc agcagcttgc gcaacgcatc ggcgatctcc
      361 tgacggccgc cgtagcccac tgccacgttg accgccaacc cgcaccgctc ccgggtctga
      421 gctgctgcgg tggtcagccg ctgcgccatc tccggcggta acaggtccag cgccccgacg
      481 actcggaccc gccacgggtt gtccggcgca cacagctcgt cgacgacatc gacgatgatc
      541 ttcagtaacg gctccacctg gtcgggcggc cggttgcgca ggttgtccgg cgacagcagc
      601 cagaacgtgg cgatctccac accggcctcg cggcaccagc cgagcagttc gaccagcttg
      661 gcagcaccaa cccggtggcc gtcgctgacg tcggcgaacc cgacctccct ggcccagcga
      721 cgattcccgt ccacgatcac cgcaacgtgg cgggggcggt tggcggtgcc gagctggcgt
      781 cgcaacctgc gctcgtagac ggcatagacg aggtcctgca gcctcacaag tcgggaaggc
      841 tacgcccggc cgttctggtg gtactggtgc accacagcgt ggcccttgcc gcgacggatc
      901 aaagcccggt tgaccggaac ggtcaccacg aatgcgacgc caagggcaac agcgagtgcg
      961 ccccagaaca gccacgagtc cagaccggct tccatcgccc ccgggatgat caatatgatc
     1021 gcgttgtcga caatctccat gacggtgatg gagacggtgt ctgcgacaag agccacgccg
     1081 atcgccgcgc tgagcgcgag gccggagcgc aacaccggaa caatcgtgaa ggaatagccg
     1141 aacaggaacg ccagcgcgat cgcaagcatg atggtcgcgc cattgcccca ccctagggcg
     1201 gtgccgatga ccaggccgag aacctcaccg atggcacagc cggtgaggca gtgcgccgtc
     1261 gcgaccaccg ccaaccggtt cagctcgcgc cgtccctgag cagtaagcgg tgcggtgccg
     1321 tgcgtggtgt gctggtgtgc atcatgatca acagagtgat cggacatgag cagatctccg
     1381 ttggccgtcg ttggcttctg cagccagtcc ccaccggcca acagtatacg gggtatgggt
     1441 atttcccacc gtcaactgtt ttcgcaaacg gccgtcagga ccggaaacta cagttgcggc
     1501 ccccgggcgc gtagatcgtc gacggtggcc atcgcctgct tgagctcacc gagccactcc
     1561 tgggcgtgct cgccgaccag g
//
LOCUS       DATADN010000376         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1406481, whole genome shotgun sequence.
ACCESSION   DATADN010000376 DATADN010000000
VERSION     DATADN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406481"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1659
                     /locus_tag="VHH53_04955"
     CDS             <1..>1659
                     /locus_tag="VHH53_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX2289539.1"
                     /translation="NNYLTVGQIYLMDNPLLREPLAAEHIKPRLLGHWGTSPGLALVY
                     AHASRLIQHTGQQMIYLTGPGHGGPTLVGASYLEGTYTEIYPHVTVDEEGLRRLFRQF
                     STPGGIPSHVSVPIPGSIHEGGELGYVLVHAFGAVIDNPDLIALAVVGDGEAETGPLE
                     GSWKQISFLNPARDGAVLPVVHLNGAKIAGPTVLARKEPDEVRSLLTGHGYAVIEVEG
                     DDLPGMHHRFAEALTRAWASIKAIQAAARGGDWDGTRPRWPMIVLRTPKGWTGPDMVD
                     GVQVSGTWRSHQVPVSGVKDNKEHLRILEDWMRSYRPEELFDEHGAPQALVLSINPNG
                     NLRMSGSPHANGGLLAVDLDLPDFRDYAVPVTAPARLRLESTRKLGELMRDTYIRNPD
                     RFRLFSPDETNSNRLGAVFEASDRAFAERVTEEDVAISRDGRVMEVLSEHACHGWLEG
                     YTLTGRHGWFATYEAFAMVSASQTVQHAKWLQEAHHLQWRAPVPSLNILLTSTAWRQD
                     HNGFSHQGPGLIQNVITHRGTISRVYLPPDANTLLSVADTCMRSR"
BASE COUNT          261 a          561 c          604 g          233 t
ORIGIN      
        1 caacaattac ctgaccgtcg gccagatcta cctgatggac aacccactgc tgcgcgagcc
       61 gttggccgcc gagcacatca agccgcggct actggggcac tggggcacca gcccgggcct
      121 ggcgctggtg tacgcgcacg cgtcgaggct gatccagcac accgggcagc agatgatcta
      181 cctgaccggt cctgggcacg gcggcccgac cctggtcggg gccagctacc tggagggcac
      241 ctacaccgag atttatccgc acgtcactgt ggacgaggag gggctgcggc ggctgttccg
      301 gcagttctcc acgccgggcg ggatccccag ccacgtgtcg gtgccgatcc cgggctctat
      361 ccacgagggc ggcgagctgg gttacgtgct ggtgcacgcg ttcggcgcgg tgatcgacaa
      421 cccggacctg atcgcgctgg cggtggtcgg ggacggggag gcggagaccg gtccgctgga
      481 ggggtcgtgg aagcagatct cgttcctgaa cccggcccgc gacggcgcgg tgctgccggt
      541 ggtgcacctg aacggcgcga agatcgccgg gccgacagtg ctggcccgca aggagccgga
      601 cgaggtcagg tccctgctga ccgggcacgg gtatgcggtg atcgaggtcg agggtgacga
      661 cctgccgggc atgcaccacc ggttcgccga ggcgctgacg cgggcgtggg cgtcgatcaa
      721 ggccatccag gcggcggccc gcggcggcga ctgggacggc acccggcccc ggtggccgat
      781 gatcgtgctg cgcaccccga agggctggac cggcccggac atggtggacg gggtgcaggt
      841 gtcgggcacg tggcgctcgc accaggtgcc ggtgtccggc gtcaaggaca acaaggagca
      901 cctgcggatc ctcgaggact ggatgcgctc ctaccggccc gaggagctct tcgacgagca
      961 cggcgccccc caggcgctgg tgctgtcgat caaccccaac gggaacctgc ggatgagcgg
     1021 cagcccgcac gccaacgggg gcctgctcgc cgtcgacctg gacctgccgg acttccgcga
     1081 ctacgcggtg ccggtgacgg cgccggcccg gctccggctg gagtcgaccc gcaagctcgg
     1141 tgagctcatg cgcgacacat acatccgcaa cccagaccgg ttccggctgt tctcccctga
     1201 cgagaccaac agcaaccggc tgggtgcggt gttcgaggcg tccgaccggg cgttcgcgga
     1261 gcgcgtgacc gaggaggacg tcgcgatatc gcgggacggc cgggtgatgg aggtgctctc
     1321 ggagcacgcc tgccatggct ggctggaggg ttacacgctg accggccggc acggctggtt
     1381 cgccacctac gaggcgttcg ccatggtgtc ggcctcgcag accgtgcagc atgcgaagtg
     1441 gctgcaggag gcgcaccacc tccagtggcg cgcaccggtg ccgagcctga acatcctgct
     1501 cacctcgacc gcgtggcgcc aggaccacaa cgggttcagc caccagggcc cgggcctgat
     1561 ccagaacgtg atcacccacc gcggcaccat cagccgggtg tacctgccgc cggacgcgaa
     1621 caccttgctg tcggtggccg acacctgcat gcggtcgcg
//
LOCUS       DATADN010000377         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1106376, whole genome shotgun sequence.
ACCESSION   DATADN010000377 DATADN010000000
VERSION     DATADN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106376"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="groEL"
                     /locus_tag="VHH53_04960"
     CDS             <1..868
                     /gene="groEL"
                     /locus_tag="VHH53_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX2289540.1"
                     /translation="EALATLVVNKIRGIFKSVAVKAPGFGDRRKAMLADMAILTGGEV
                     ISEEVGLKLENADLSLLGRARKVVVTKDETTTVEGSGDPEQIQGRVNQIRNEIDNSDS
                     DYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVRNAKAAVEEGIVPGGG
                     VSLMQASALAFEKLDVTGDEATGANIVKVALEAPLKQIAINAGLEGGVVAEKVRGLPA
                     GHGLNAATGEYEDLIKAGIIDPAKVTRSALQNAASIAALFLTTEAVIVDKPEKNPAPA
                     GGGMPDGGGMDF"
     gene            complement(1023..>1601)
                     /locus_tag="VHH53_04965"
     CDS             complement(1023..>1601)
                     /locus_tag="VHH53_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2289541.1"
                     /translation="TVGRPLPQVEVRVADPASGQTQALGAVGEVLIRGYQVMAGYHGL
                     PEATAAAIDAEGWLHTGDLGLMDACGNLRIEGRLKDMIIRGGENIYPREIEEFLYTHP
                     DILDAQVIGVPDARHGEELCAWVRLRPGAPELTADALRDYATGKLAHYKFPRYVHVVR
                     EFPMTVTGKVRKVEMRERSVQLLGLRPTTKHA"
BASE COUNT          270 a          521 c          552 g          258 t
ORIGIN      
        1 tgaggcgctg gccaccctgg tcgtcaacaa aatccgcggc atcttcaagt cggtcgcggt
       61 caaggcgccc ggcttcggtg atcgccgcaa ggcgatgctg gccgacatgg ccatcctcac
      121 cggtggtgag gtgatcagtg aggaggtcgg gctcaagctg gagaatgccg acctgtcgct
      181 gctgggccgg gcccgcaagg tcgtcgtcac caaggacgag accaccaccg tggagggttc
      241 cggcgacccc gagcagatcc agggccgggt caaccagatc cgcaacgaga tcgacaactc
      301 ggactccgac tacgaccgcg agaagctgca ggagcggctg gccaagctgg ccggcggcgt
      361 tgcggtgatc aaggccggag cggccaccga ggtggagctc aaggagcgca agcaccgcat
      421 cgaggacgcg gtgcgcaacg ccaaggctgc cgtcgaggag ggcattgtcc ccggcggtgg
      481 tgtctcgttg atgcaggcca gtgcgctggc cttcgagaag ctcgacgtga ccggtgacga
      541 ggcgaccggc gccaacatcg tcaaggtggc gttggaggct ccgttgaagc agattgctat
      601 caacgccggt ctcgagggtg gcgtggtggc cgagaaggtg cgcggcctgc cggccgggca
      661 cggcctcaac gcggccaccg gtgagtacga ggacctgatc aaggccggca tcatcgaccc
      721 ggccaaggtc acccgctcgg cactgcagaa cgcggcgtcg atcgcggccc tgttcctcac
      781 cacggaagcc gtgatcgttg acaagccgga gaagaacccg gcaccggcgg gcggcggcat
      841 gcccgacggc ggcggcatgg acttctgatc cgctagctgc acaccacagg ggtcgctcct
      901 ttctctagga gcggcccctg tgctttgtgc tgccgcctgt ggagtgcgat aagcatccat
      961 gtcgccgtcc agatggcctt gcggacagcg acaagcatcc atatcgcgtc gcgagcgcgg
     1021 ggttaggcgt gcttggtggt ggggcgcagg ccgagtaact gcaccgagcg ttcgcgcatc
     1081 tccaccttgc ggactttgcc ggtgacggtc atcgggaact cgcgcaccac gtgcacgtac
     1141 cgcgggaact tgtagtgcgc gagcttgccg gtggcgtaat cccgcagcgc gtcagcggtc
     1201 agttcgggtg cgcccggtcg caaccgcacc caggcgcaca gctcctcgcc gtggcgcgcg
     1261 tccggtacgc cgatcacctg cgcgtccagg atgtccgggt gggtgtagag gaactcctcg
     1321 atctcccgtg ggtagatgtt ctcaccgcca cggatgatca tatccttgag tcggccttcg
     1381 attcgcaggt tgccgcacgc gtccatcaag cccaggtcac cggtgtgcag ccagccttca
     1441 gcatcgattg cggccgcagt cgcttccggt agcccgtggt agccggccat cacctggtag
     1501 ccgcggatca ggacctcgcc gaccgccccc agagcctggg tctggccgct tgccgggtcg
     1561 gccacccgga cctcgacctg cggcaacgga cggccgaccg t
//
LOCUS       DATADN010000378         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1406690, whole genome shotgun sequence.
ACCESSION   DATADN010000378 DATADN010000000
VERSION     DATADN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406690"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VHH53_04970"
     CDS             complement(<1..1559)
                     /locus_tag="VHH53_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2289542.1"
                     /translation="MPKLATAAKRLLVGRPFRSDRLAHTLLPKRIALPVFASDPMSSV
                     AYAPEEILLVLSVAGASAYVFSPWIALFIALVMIAVVASYRQNVRAYQSGGGDYEVAT
                     VNHGPRWGLMVASALLVDYVLTVTVSIASAAANIGSAIPYVGTHKVEFAVAAILVLTA
                     MNLRGVRESGAAFAIPTYAFVAGILVMILVAFYRAFLLGHEVRAESADLQLVEESGHL
                     AGLGFAFLILRAFSSGSAALTGVEAIANGVPAFRKPKGRNAATTLLMMGVLAITMFLG
                     LIMLARLTGAQIAEDPARQLINAPAGYEQKTLVAQLANAVFGGFAPATWYVIFFTGLI
                     LLLAANTAFNGFPVLGSILAQERYLPRQLHTRGDRLAFSNGIVFLAGFAIVLMVAFDA
                     EVTRLIQLYIVGVFMSFVISQSGMIRHWNRLLRTETDPTARRRMRRSQAINAFGFSMT
                     ALVLVVILITKFTEGAWISLAAMAAIYAVMTAIRRHYDRVAAELVAGEGDGVLPSRNH
                     AIVLVSTLHKPT"
BASE COUNT          301 a          588 c          590 g          227 t
ORIGIN      
        1 gtcggcttgt gcagcgtgga gaccagcacg atcgcgtggt tgcgcgacgg cagcacgccg
       61 tcgccctccc ccgccaccag ctccgcggcg acccggtcgt aatgccgccg gatggcggtc
      121 atgaccgcgt agatcgccgc catggcggcc agcgagatcc acgcgccctc ggtgaacttc
      181 gtgatcagga tcaccacgag gaccagcgcg gtcatcgaga agccgaacgc gttgatcgcc
      241 tgcgaacggc gcatgcgccg ccgggcggtc gggtcggtct cggtgcgcag caaccggttc
      301 cagtgccgga tcatgccgct ctgactgatc acgaaggaca tgaacacgcc gacgatgtac
      361 agctggatca gccgggtgac ctccgcgtcg aaggccacca tgagcacgat ggcgaatccg
      421 gcgaggaaca cgattccgtt ggagaacgcc agccggtccc cccgggtgtg cagctgccgc
      481 ggcaggtagc gctcctgggc caggatcgag ccgagcaccg ggaacccgtt gaacgcggtg
      541 ttggccgcca gcagcaggat cagcccggtg aagaagatca cataccaggt cgccggtgcg
      601 aacccgccga acacggcgtt ggccagctgg gcgaccaggg tcttctgctc gtagcccgcc
      661 ggcgcgttga tcagctggcg ggccgggtcc tcggcgatct gggcaccggt gagccgggcc
      721 agcatgatca gcccgaggaa catcgtgatc gccagcacac ccatcatcag cagcgtggtc
      781 gccgcgttgc ggcccttggg cttgcggaac gccggcacgc cgttggcgat cgcctcgacc
      841 ccggtgagcg cggcgctgcc ggacgagaac gcccgcagga tcaggaaggc gaagcccagg
      901 ccggccaggt gcccggactc ctcgacgagc tgcaggtcgg cgctctccgc gcgcacctcg
      961 tggccgagca ggaaagcccg gtaaaaggcg accaggatca tcaccagaat cccggcgacg
     1021 aacgcgtagg tcggaatcgc gaaggcggcg cccgactcgc gcaccccgcg caggttcatg
     1081 gcggtcagga ccaggatcgc ggcgacggcg aactcgacct tgtgcgtgcc cacgtacggg
     1141 atggctgaac cgatgttggc cgccgccgag gcgatcgaca ccgtcaccgt gagcacgtag
     1201 tcgaccagca gcgcgctggc caccatcagg ccccaccggg ggccgtggtt gacggtggcg
     1261 acctcgtagt caccgccgcc ggactggtag gcccgcacgt tctgccggta cgacgccacc
     1321 accgcgatca tgaccagcgc gatgaacagc gcgatccacg ggctgaagac gtaggcggat
     1381 gcccccgcga ccgacagcac gagcaggatc tcctccggcg cgtacgccac gctcgacatc
     1441 gggtcggagg cgaacaccgg gagcgcgatg cgcttgggca gcagcgtgtg ggccagccgg
     1501 tcgctgcgga acggccgacc aaccaggagc cgcttggccg cggttgcgag cttgggcacg
     1561 actgcacagc gtagggctat cggtcaccgc aacggagtag tgcccaagct cgcaaccgcg
     1621 gccaagcggc tcctggttgg tcggccgttc cgcagcgacc ggctggccca cacgctgctg
     1681 cccaagcgca tcgcgctccc ggtgtt
//
LOCUS       DATADN010000379         4585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1240571, whole genome shotgun sequence.
ACCESSION   DATADN010000379 DATADN010000000
VERSION     DATADN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240571"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..336
                     /locus_tag="VHH53_04975"
     CDS             151..336
                     /locus_tag="VHH53_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HEX2289543.1"
                     /translation="MYFTDRGIEELEDRRGNEQLNVEWLAARMRAFVDEHPEFEQAVE
                     RLATFLARDDADEPDTL"
     gene            complement(468..1460)
                     /locus_tag="VHH53_04980"
     CDS             complement(468..1460)
                     /locus_tag="VHH53_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2289544.1"
                     /translation="MEYTQLGSSGLSVSRLCLGTMNFGPETSETDSHAIMDRAHEHGI
                     NFFDTANVYGWQKGEGVTEKIIGRWFAAGGGRRERTVLATKLYGSMGNWPNETKLSAL
                     NIRRACDASLRRLQTDYIDLYQMHHVDRATPWEEIWEAMSVLRQQGKIIYVGSSNFAG
                     WHLAAAQEAARRQRVTGLVSEQSIYNLLNRWVELEVLPAARHYGLGLIPWSPLNRGLL
                     GGVLRKQREGGGSRSTSGSSADALAANRERIEAYEKLCADLGEDPANVGLAWLRHQRG
                     VTAPIVGPRTVTQLDSSLRAVTITLDAEVLDRLDDQFPPPGPNSSKPAPEAYAW"
     gene            complement(1555..2430)
                     /locus_tag="VHH53_04985"
     CDS             complement(1555..2430)
                     /locus_tag="VHH53_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2289545.1"
                     /translation="MTRIMTLVMAVVLAAAVTACGSGNPLAGGGNGSAIVVGSADFAE
                     SELLMEIYAAALRKAGIQVQTRPRLGSREITNVAVRDGSITVMPEYSGNLLQSVDPNA
                     TATAADEVYAALQRSLPQGLEVLEQSAAEDSDVLVVTQQTAATLGLTSMDQLGPHCGQ
                     LTLGAAGEWPGRWKDKIAQIYGCTFREILSTDVAGPVTLEALRSGDAEIVNLNTTSPD
                     IAAHGWVELADPKNMYPAQRILPLVRAGALNQAGVDALNEVSAALTTENLTELNQRML
                     QERAVPADLAKDFVS"
     gene            complement(2470..3159)
                     /locus_tag="VHH53_04990"
     CDS             complement(2470..3159)
                     /locus_tag="VHH53_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2289546.1"
                     /translation="MFSQVLAWFADAAHWRGAGGVGVRIAEHLYYTAVTVLIASVVAV
                     PLGLLVGHTARGGVLLVGLANAMRALPTLGLLTFLFLLFRSQGVSTVVALVVLAVPPV
                     LAGAYAGVQSVEAEVVDAARGMGMTSAQRLFQVEVPGALPLLLGGLRSAVLQVVATTT
                     VAAYLGLGGLGRLLLDGLRSGDYPQMVAGALLTAGLAVVLDLLLAGVQQLAVPTGVRL
                     SSARATSRGTG"
     gene            complement(3159..3836)
                     /locus_tag="VHH53_04995"
     CDS             complement(3159..3836)
                     /locus_tag="VHH53_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289547.1"
                     /translation="MFSDISGYLSSPSNRAKLLELVGEHVVLAVLPLLIGLSVAVPLG
                     WAAQRMRWLRAVVLGSASVLYTVPSLALFVIMPSILGTRILDGTNVVVALALYTSALL
                     VRPVVDALDAVPGHVVAAATAMGYQPVRRFVAVELPMAVPVLTAGVRVASVSNISLVS
                     VGALIGVGGLGQLFTEGFQAQYLAPIMIGIVLTVVLAVAIDLLLVGLRRVSTPWTRAQ
                     LGAGAGT"
     gene            complement(3826..>4585)
                     /locus_tag="VHH53_05000"
     CDS             complement(3826..>4585)
                     /locus_tag="VHH53_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2289548.1"
                     /translation="TGATRRAARARAAELIELVGLPAELGRRYPAQLSGGQQQRVGVA
                     RALAADPPVLLMDEPFSAVDPVVRADLQDQLQRLQGELGKTILFVTHDIDEAVKLGDA
                     VAVLRAGGVLAQFGAPQQVLAEPADDFVAGFVGRDRGYRAMSFVCSGVLPVHELDTVR
                     VGEAGQGHGWRLAVDAHGRPRGWLAPVGADGTLRAGGSLHRLGGPMRTALDAALSSPS
                     GFGVVVDGNDAVAGGVRAEEVLALWRRQRGVDVQ"
BASE COUNT          844 a         1627 c         1419 g          695 t
ORIGIN      
        1 ccccacctca cccccggcgt gtccggccct agtgtccgcg tgtccggccc tagtgtccgg
       61 cgtatccggc cctagtgtcc gtgtatccag cgttggtgca cgctgcgccg ccgttctgcg
      121 cgcaggcgcc tcggaagaag aggagtcgag gtgtatttca ccgaccgcgg tattgaggag
      181 ctcgaagatc gacgcggcaa cgaacagctc aacgtcgagt ggctggccgc gcgaatgcgc
      241 gcattcgttg atgagcaccc ggagttcgag caggccgtcg agcggttggc cacgttcctg
      301 gctcgcgacg atgccgatga gccggacacc ctttgagggc gaagggcgct agtgatcgtc
      361 agccatccgc cactccttac gacgacggtg cacgccagga acagataatg cgctcaaacg
      421 ccggacacac cgtgcaggag aggacgacac gccagggggc acgggtgtta ccaggcgtag
      481 gcttccgggg caggtttgga actgttgggg cctggcggag ggaactgatc gtcaagccgg
      541 tccaggacct cagcatccag tgtgatggtg acggcccgca gcgagctgtc cagttgcgtg
      601 accgttcgcg ggccgacaat cggcgcagtc accccgcgct ggtgccgcaa ccaggccaaa
      661 ccaacgttgg cggggtcctc gccgaggtcg gcgcacagct tttcgtaggc ctcgatgcgc
      721 tctcggttcg ccgcgagcgc atcggccgag gagcccgagg tactccgcga accgccgccc
      781 tcacgttgct tgcgcagcac accgccgagc agaccacgat tcagtggtga ccacgggatc
      841 agcccaaggc cgtagtggcg cgcggcgggc agcacctcca gctccaccca gcgattgagc
      901 aggttgtaga tcgactgctc gctgaccagc cccgtgacgc gctggcggcg agccgcctct
      961 tgggcggccg cgaggtgcca gccggcgaaa tttgacgagc cgacataaat gatcttgccc
     1021 tgttgccgga gcaccgacat ggcttcccag atctcctccc acggggtggc ccgatccacg
     1081 tggtgcatct ggtagaggtc gatgtagtcg gtctgcagcc ggcgcagcga cgcgtcacag
     1141 gcccgccgga tgttcagcgc ggaaagcttg gtttcgttgg gccaattccc catcgagcca
     1201 tagagcttgg tcgccaacac ggtgcgctcg cggcgaccgc cgccagcggc gaaccagcgg
     1261 ccgatgatct tctcggtgac accttcgcct ttctgccagc cgtagacgtt cgcggtgtcg
     1321 aagaagttga tgccgtgctc gtgggcccga tccatgatcg cgtgactgtc ggtctcggag
     1381 gtctccgggc cgaaattcat cgtgcccagg cacagtcggg agaccgaaag tccactgctg
     1441 cccagctgcg tgtattccat gccgccagcc tcgcactgcg accgtcatcg gaccacccga
     1501 ccgtggtcgt gagtggtcgt gagtcgcatt gagtgctgta acaccgttac gcactcacga
     1561 gacgaagtcc ttggccaggt cggcgggtac cgcccgttcc tgtagcatcc gctggttgag
     1621 ttcagtgaga ttttcggtgg tcagtgcagc tgagacctcg ttgagcgcgt caacacccgc
     1681 ctggttcagt gccccggcgc ggaccagcgg aaggatgcgc tgagccgggt acatgttctt
     1741 cgggtccgcc aactcgaccc agccgtgagc ggcgatgtct ggcgaagtgg tgttcaggtt
     1801 cacgatctca gcatccccac tgcgtagtgc ctccagcgtc accggaccgg ccacatcggt
     1861 gctgagaatc tcgcggaaag tgcatccata gatttgagcg atcttgtctt tccagcggcc
     1921 cggccactcg cccgcggcgc ccagggtaag ttgcccgcag tgcggtccga gctggtccat
     1981 gctggtaagc cctagcgtcg cagcggtctg ctgggtgact acgaggacgt cggaatcctc
     2041 ggccgcagac tgctcgagca cttctaaacc ctgcggtagg gaccgctgca gcgcggcgta
     2101 cacctcatct gccgccgtcg cggtcgcgtt cgggtcgacc gactgcagca ggttgccgga
     2161 gtactcgggc atcacggtga tcgacccgtc ccgcacagcg acgttagtga tctcgcggct
     2221 acccagccga ggcctggttt gcacctggat acccgccttg cgcagcgccg cggcgtaaat
     2281 ttccatcagc aactcgctct cggcgaaatc ggccgagccg accacgatgg cgctgccgtt
     2341 gccgccccca gcaagcgggt taccggatcc gcaagcagtg acagcggccg ccagcacaac
     2401 ggccatgacc aacgtcatga tacgtgtcat cgatcctcat cctacgaatc ccggaatccc
     2461 cgcgaatcct cagcccgtac cccgcgaggt cgccctcgcc gaagacagtc gcactccggt
     2521 gggcactgcg agctgctgca ccccggccag cagcaggtca agcactaccg caagcccggc
     2581 ggtgagcaga gccccggcca ccatctgcgg ataatccccc gaacgcagcc cgtcaagcag
     2641 cagccggccc aggccaccta gccccaggta ggccgccacc gtggtggtcg caaccacctg
     2701 cagcaccgcg ctgcgcagtc caccaagcag caacggaagc gcccctggca cttcgacctg
     2761 aaacaggcgt tgcgccgatg tcatacccat cccccgcgca gcatcgacca cctcggcctc
     2821 gacgctttgc accccggcgt aggccccggc aagtaccggt ggcacggcca gcacgaccag
     2881 tgcgaccact gtggagactc cctgcgagcg aaacagcaaa aagagaaacg tcagcaagcc
     2941 caacgtcggc agtgcccgca tcgcgttggc cagtcccacc agcagcaccc cgccacgagc
     3001 ggtgtgcccc accaacaacc cgagcggaac ggcgaccacc gacgcgatga gcaccgtgac
     3061 ggcggtgtag tacaggtgct cggcgatccg cactccgacg ccgccagcac cgcgccagtg
     3121 cgccgcgtcg gcgaaccagg ccagaacctg ggaaaagatc acgtgccggc tcctgcccct
     3181 agctgcgccc gcgtccaggg agtactcacc cgacgcaaac cgaccagcag cagatctatc
     3241 gcgaccgcca gcacgacagt cagcacgatg ccgatcatga tcggtgccag gtactgggcc
     3301 tggaagccct cggtgaacaa ctggcccagc cccccgacac cgatcaatgc ccccacactg
     3361 accaagctga tgttgctcac cgacgccacc cgcacaccag cggtcagtac cggaacagcc
     3421 atgggcagct cgacggcgac gaatcgtcgc acgggttgat agcccatcgc ggtggctgcg
     3481 gccaccacgt gtccgggcac tgcatccagc gcgtcgacca ctggacgcac cagcagcgca
     3541 gatgtgtaca gggcaagcgc gaccactacg ttcgtaccgt ccaggatccg ggtgcccagg
     3601 atgctgggca tgatcacgaa cagggccagc gacggcactg tgtacagcac gctggccgac
     3661 ccgagcacca cggcccgcag ccagcgcatc cgctgcgctg cccatcccag cggcaccgcc
     3721 accgacagtc cgatgagcag cggcaggacc gcgagaacga catgctcgcc gaccaattcg
     3781 agtaatttgg cgcgattgct tggactgctc agatagccgc tgatgtcact gaacatcgac
     3841 gccccgctgc cggcgccaca gcgcgagcac ctcctcggcg cggacaccgc ccgccacggc
     3901 atcgttgcca tcaaccacca cgccgaaccc cgaaggtgag gacagcgccg cgtccagcgc
     3961 agttcgcatc ggaccaccca ggcggtgcag cgaaccgcca gcccgcagcg tgccgtcggc
     4021 acccaccgga gccaaccagc cacgcggcct gccatgggcg tccacggcta gccgccaccc
     4081 gtgcccttgc cctgcctcgc ccacccgtac cgtgtccagc tcgtggaccg gaagaacacc
     4141 tgagcagacg aacgacatcg cgcggtagcc acggtcgcgt ccaacgaatc cagcgacgaa
     4201 gtcgtcggcg ggctcagcga gcacctgctg cggcgcgccg aactgggcca gcactccccc
     4261 agcccgcaac actgcgacgg catcgccgag cttgaccgcc tcgtcgatgt cgtgggtgac
     4321 gaacaggatg gtcttgccca gctcgccttg cagtcgctgc agctgatctt gcaggtcagc
     4381 ccggaccacc ggatcgacag cggagaacgg ctcatccatc agcagtaccg gtggatcagc
     4441 ggccagcgcg cgggccaccc ccacccgctg ctgctggccg ccggacaact gtgccgggta
     4501 ccgccggccc agctcggcgg gcaacccgac gagctcgatc agttcggcgg cccgggctcg
     4561 ggccgcccgc cgggtagcgc cggtc
//
LOCUS       DATADN010000380         3356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_648018, whole genome shotgun sequence.
ACCESSION   DATADN010000380 DATADN010000000
VERSION     DATADN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648018"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VHH53_05005"
     CDS             <1..570
                     /locus_tag="VHH53_05005"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX2289549.1"
                     /translation="DTDAKLFFALGNALLDASIAAWAAKYRWDFVRPITAIRSRYKGK
                     PVTSWLGPYQGYGQVPGEQWIPYQSPNVVSPPFPEYVSGHSTFSSAAAAILAKFTGSD
                     SFGAGVTIEAGTSLFEPRTETHAGTPATNILLSWPTLTAAAKDAGLSRRLGGIHFQDG
                     DEHGYNLGQRVGGNVLARTMGYVNGTVAG"
     gene            680..1219
                     /locus_tag="VHH53_05010"
     CDS             680..1219
                     /locus_tag="VHH53_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289550.1"
                     /translation="MMRGATSVLRRLAVLVTLGLAVAVPAAAQTDPARLAVTATSNRC
                     AGGVPVVDYTVTNQWSTPVSVQTWWVADASVSGIAAVHDLGPAPDGAAGAFSLPFAFY
                     STVTVYATASWPDGKVTTNASWAIPVASCDAPTSPPTTVVEPPTSTTVDDGGGGNGNG
                     NGNGNGHGNRDTTTTIELG"
     gene            complement(1251..1766)
                     /locus_tag="VHH53_05015"
     CDS             complement(1251..1766)
                     /locus_tag="VHH53_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX2289551.1"
                     /translation="METWDAIRSRRNVRSYQERAIAPEDLDRILEAGRRSPSAGNQQA
                     WDFVVCTDREQLTQLARVWRGAGHVARSAATIALVAPNPDDARSRDLIQYDLGQATIS
                     VMLAAADLGIGSAHAAVSDQELARRLLGVPEDRFCAWLISLGVPADRPLTPIEQPNRR
                     PFDDVVHRGHW"
     gene            2053..2364
                     /locus_tag="VHH53_05020"
     CDS             2053..2364
                     /locus_tag="VHH53_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX2289552.1"
                     /translation="MTNNGTADVTTTSSSTPTAGPGRFIAEVRVMSFGTRDLAAAADA
                     DLLRLGMVVCDGLGIESLGFRRVVQRLVQSEAHPTTTEATALVSSAMRNLCPQHATTT
                     T"
     gene            2685..>3356
                     /locus_tag="VHH53_05025"
     CDS             2685..>3356
                     /locus_tag="VHH53_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2289553.1"
                     /translation="MNSEHSAQRVALVAGATRGAGRAIAVELARTGLYVYATGRSSRA
                     AGRSEIGRPETIEETGDLLAAAGGHGTALVVDHEDPNAVAGLAARIEAERGRLDVLVN
                     DIFGGDRYAQWHRPMWEHDLAGGLRMLRMGVDTHIITAHAALPLLLRGDRGLLVEMTD
                     GTTEVNAHYRERVGFFYDLVKANVSRIVLGLAHELRGRSVTALGVTPGWLRSENMLDH
                     YGVTEE"
BASE COUNT          510 a         1200 c         1138 g          508 t
ORIGIN      
        1 gacaccgacg ccaagttgtt cttcgccctc ggcaatgcgt tgctggacgc gagcatcgcg
       61 gcctgggcgg ccaagtaccg ctgggacttc gtccggccga tcaccgccat ccggtcccgc
      121 tacaagggca agccggtcac ctcctggctc gggccgtacc agggctacgg gcaggtcccc
      181 ggggagcagt ggatcccgta ccagtcaccc aatgtggtca gcccgccgtt ccccgagtac
      241 gtctccggcc acagcacctt cagctcggcc gccgcggcca tcctggccaa gttcaccggc
      301 agcgacagct tcggggccgg tgtgaccatc gaggctggca cctcgctgtt cgagccgcgg
      361 accgagaccc acgccggcac cccggccacc aacatcctgc tgagctggcc gaccctgacc
      421 gccgcggcca aggacgccgg actgtcacgc cgccttggtg gcatccactt ccaggacggc
      481 gacgagcacg gctacaacct cggccagcgg gtcggcggca acgtgctcgc caggaccatg
      541 ggctacgtca acgggaccgt cgccggatga cccggaggta ccggtgccac ctcgagctgc
      601 ctgggtgtaa cgccggcggg ctgctcgccg ctcttctccg aatggaggga tgtcctcgtt
      661 ccaccggaac aagaccacga tgatgagggg ggccacctcc gtgctacgca gactcgccgt
      721 gctcgtcacc ttgggattgg ccgttgcggt cccggccgcc gcgcagaccg atccagcccg
      781 gttggcggtg accgcgacct ccaaccgctg cgccgggggg gtgccggtgg tcgactacac
      841 cgtgaccaac cagtggtcca cgccggtgtc tgtccagacc tggtgggtgg ctgacgcatc
      901 cgtctccggc atcgcggccg tccacgacct cgggcccgcc cccgacggcg ccgccggggc
      961 cttcagcctg ccgttcgcgt tctattccac cgtgaccgtg tacgccaccg ccagctggcc
     1021 ggacggcaag gtcacgacca acgcctcctg ggcgatcccc gtggccagct gtgacgcccc
     1081 aacctctccg cctaccacgg tcgtcgagcc gcccacatcc accacggttg acgacggtgg
     1141 cggcggcaac ggcaacggca acggcaacgg caacgggcac ggcaaccgcg atacgacgac
     1201 gaccatcgag ctcggctaga acaccggcac cgactccgac tctgcggtga ctaccagtgg
     1261 ccgcggtgca cgacgtcgtc gaacgggcgc cggttgggct gctcgatcgg ggtgagcgga
     1321 cgatcggcgg ggacgccgag gctgatcagc caggcgcaga accggtcctc gggaacgccc
     1381 agcagccgcc gggccagctc ctggtcgctc accgccgcat gcgcgctgcc gatgcccagg
     1441 tcggccgcgg ccagcatgac gctgatcgtc gcctggccca ggtcgtactg gatcaggtcg
     1501 cgtgagcggg catcatcggg gttcggggcg acaagggcga tcgtcgccgc cgagcgggcg
     1561 acgtggccgg cgccgcgcca caccctggcc agctgggtga gctgctcgcg gtcggtgcag
     1621 accacgaagt cccacgcctg ctggttgccg gccgaggggc tccgccgccc ggcctccagg
     1681 atccggtcga ggtcctcggg ggcgatcgcc cgctcctggt aggaccgcac gttgcgccgg
     1741 gacctgatcg catcccatgt ctccacccct cgacctccca cagagtggtg acgattctgc
     1801 tcccaccaac ccgggagcag ggccgctcgg ctcgagcctt ctgatcgtac tgctcaccgc
     1861 tcgcgccccg gcggttgctt gcagcctgtg cgatgtggcg gcgcctcact gtaacgctgc
     1921 ttggtgggct ggcgctctca gaaccgaagc gtttcagggg gggcgaagca attgtgcgtg
     1981 gcaggccgct ggctgatgag gcatgtcgtc ctacttggcc tcctgagtct cgtcagcaca
     2041 ggatgcggcc aggtgacgaa caacggcacg gccgacgtga ccaccacctc ctcgtccacc
     2101 ccaacggcag gaccgggacg cttcattgcc gaggtcaggg tgatgagctt cggcaccaga
     2161 gacctcgctg ccgccgccga tgcggacctg ttgcgacttg ggatggtcgt ctgcgacggc
     2221 ttgggcatcg agagcctggg ctttcgtcgg gtcgtgcagc gactggtgca gtcggaggca
     2281 cacccgacga ccaccgaagc cacggcgctt gtcagcagtg cgatgcgcaa cctctgtcca
     2341 cagcacgcca ccaccaccac ctgaccaggc gggacacacg cctggtacac gcggcgccgg
     2401 caggtagacc gtggagcggg cacgactgac attcggccgt cggttggaag accaagcgtc
     2461 agtggcccag ctgctctcgg gcctcgcggc tcgtcatcac cccgaccatc agggtgtcgg
     2521 tgaacgggtc cacgctgtcg ccctccccgt ttgggccagg caccgacagc acgaccatcc
     2581 ccgggctcgt tcgacgattc tggtctgcct tgtcggcgct ttgcggttcc cggagcagat
     2641 tcggcgggac gtcgtggtgg cgccgtcatg ctttcatggt cgccatgaat tccgagcaca
     2701 gcgcccagcg ggtggcgctg gtggcggggg caacgcgggg ggcgggacgt gccatcgcgg
     2761 tcgagctggc ccggaccggg ctgtacgtct acgccaccgg gcgcagcagc cgcgcggccg
     2821 ggcggtcgga gatcgggcgg ccggagacga tcgaggagac cggcgacctc ttggccgcag
     2881 ccggtggcca tggcacggcc ctggtcgtcg accacgagga ccccaacgcc gtcgccggcc
     2941 tggccgcacg gatcgaggcc gagcgcggcc gcctggacgt cctggtcaac gacatcttcg
     3001 gcggcgaccg ctacgcccag tggcaccggc caatgtggga gcacgacctc gccggtggcc
     3061 tgcgaatgct gcgcatgggt gtggacaccc acatcatcac cgcgcatgcc gccctgccgc
     3121 tgctgctgcg cggcgaccgc gggctgctgg tcgagatgac cgacggcacg acggaggtca
     3181 atgctcacta ccgcgagcgg gtcgggttct tctacgacct ggtcaaggcg aacgtgagcc
     3241 ggattgtcct cgggctcgcc catgagctgc gcggccggtc cgtcaccgcg ctcggagtca
     3301 ctcccgggtg gctgcgctca gagaacatgc tcgaccacta cggcgtcacc gaggag
//
LOCUS       DATADN010000381         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1407538, whole genome shotgun sequence.
ACCESSION   DATADN010000381 DATADN010000000
VERSION     DATADN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407538"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VHH53_05030"
     CDS             <1..753
                     /locus_tag="VHH53_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2289554.1"
                     /translation="IVCFPATRDHDLVPLLRAARPLPADVCVVPRLYELGAALPRGYL
                     DELHDIPLVPLRRLGHGRAGRAAKAGFDFAVAAALLVAVAPLLLVLAVAIRLNGRPAL
                     FRQTRLTRAGKTMELVKLRSMIDHADSDTRWAVLPEHTTSLGGWLRATHLDELPQLVN
                     VLRGEMSLVGPRPERPYFAAQFAAQIPRYADRHRMRTGLTGWAQVHGLHGDTSITDRI
                     RLDNHYIEYWSPWMDMTILARTLRPVSSGGSK"
     gene            750..1928
                     /locus_tag="VHH53_05035"
     CDS             750..1928
                     /locus_tag="VHH53_05035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289555.1"
                     /translation="MKVLHVITGLGVGGAELQLRSLLQQTRNQSDVVTLYNPGVVAAM
                     ISGDGVRVHDLGMTRNTELPALLRLARLIRAGGYDVVHAHLYRACIYGRLAARLASTP
                     VVVTTEHSIGETHLERRRMTLGVRALYLGTDLCSDATIAVSPAVAQRLTKWGIAARKI
                     EIIPNGLDFGRVAFDAGSRDRIRSEFDLPLDAYLIGVLGRLDPIKRFDLVIRAAAPLL
                     DEQRRLLIVGDGQIRPELEAEAQRQGVADFVTFAGERHDVAAMLSSLDLFIASSEQET
                     FGLSVLEALANGIPALYTTCPALDGIVTERARQVPGEVEGLRQEIEAELVHGPRPREE
                     VPAIREHYGIEAVTRRIDDLYDRLMAGKQHRHRPDDSRALADSIGPDSIGPNSTGGNR
                     "
     gene            1925..>2034
                     /locus_tag="VHH53_05040"
     CDS             1925..>2034
                     /locus_tag="VHH53_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2289556.1"
                     /translation="MKALITGGAGFIGSAVVRHLISNTEASVVNVDKLTYA"
BASE COUNT          319 a          723 c          671 g          321 t
ORIGIN      
        1 atcgtgtgct tccccgctac ccgggaccac gacctggtac cgctgctgcg agcggcccgc
       61 ccgttgccgg cagacgtctg cgtggtgcca cggctttacg agctgggcgc agcgctgccc
      121 cgtggttacc tcgacgagtt acacgacatc ccgctggtgc cgcttcgccg cctcgggcac
      181 ggtcgggccg ggcgcgccgc caaggccggc ttcgacttcg ccgtcgctgc ggcgctactg
      241 gtcgccgtcg ctccgctgct gctggtcctc gcggtcgcga tccggctcaa tggtcgcccc
      301 gcgctgttcc ggcagactcg cctcaccagg gccgggaaga ccatggagct ggtcaagctg
      361 cgttccatga tcgaccacgc ggactccgac acccgctggg cagtcctgcc cgagcacacc
      421 acctcgcttg gtgggtggct gcgtgccacc cacctcgacg agctcccgca actcgtcaac
      481 gtgttgcgcg gagagatgtc cctggtgggg ccccgaccgg aacggccgta cttcgcagcg
      541 cagttcgcgg cgcagatacc gcggtacgcc gaccggcacc ggatgcggac cggcctgacc
      601 ggatgggccc aggtgcacgg gctgcacgga gacacctcca tcactgaccg gatccgcttg
      661 gacaaccact acatcgagta ctggtcgccc tggatggaca tgacgatctt ggcccgcacg
      721 ctgcgccccg tgtcgtcagg aggaagcaag tgaaagttct gcacgtgatc accgggttgg
      781 gtgtgggagg cgcggagctg cagctgcggt cactgctaca gcagacccgc aaccagtccg
      841 acgtcgtgac gctctacaac cccggtgtag tcgccgcgat gatctccggg gacggcgtgc
      901 gggtgcacga cctggggatg actcgcaaca ccgagctgcc cgcgctgctg cggctggccc
      961 ggctgattcg cgccggtggc tacgacgtgg tgcacgccca cctgtaccgg gcctgcatct
     1021 acgggcggct cgcggcccgg ctggccagca cgccggtggt ggtgaccact gagcattcca
     1081 tcggcgagac ccacctcgag cgcaggcgga tgacgctggg ggtacgtgcc ctgtacctgg
     1141 gcaccgacct gtgctccgac gccacgatcg cagtgtcccc cgcggtggca cagcgcctga
     1201 cgaagtgggg gatagcggca cgcaagatcg aaatcattcc caacggcctc gacttcgggc
     1261 gggtcgcctt tgacgccggc tcccgggacc ggatccgcag cgaattcgac ctcccactcg
     1321 acgcttacct catcggtgtg ctgggccggc tcgacccgat caagcggttc gacctcgtga
     1381 tccgggctgc ggcgccgctg ctcgacgagc aacgccggct gctgatcgtc ggcgacggcc
     1441 agatccggcc cgagctcgag gccgaggcgc aacgccaggg cgtcgcggat ttcgtgacct
     1501 tcgccgggga acgccacgat gtggccgcga tgctgtccag cctggacctg ttcatcgcgt
     1561 cctcggaaca ggaaacgttc gggctgtcgg tcctcgaggc cctggccaac gggatacccg
     1621 cgctctacac gacctgccct gcactggacg gcatcgtcac cgagcgcgcc cgccaggtac
     1681 ccggcgaggt cgaggggctg cgccaggaga tcgaagccga actggtccac gggccccgcc
     1741 ctcgcgagga ggtgccggcc atccgcgagc actacggcat cgaagcggtg acccgccgga
     1801 tcgacgacct ctacgaccgg ctgatggcgg gcaaacagca ccgtcaccga cccgacgact
     1861 cccgagcgct ggccgattcc attgggccgg attccatcgg gcccaactcc accggaggca
     1921 accgatgaaa gcgctgatca ccggtggggc cggtttcatc ggctccgccg tcgtccgtca
     1981 cttgatctcg aatacggagg ccagcgtcgt caacgtcgac aagctgacct acgc
//
LOCUS       DATADN010000382         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1407723, whole genome shotgun sequence.
ACCESSION   DATADN010000382 DATADN010000000
VERSION     DATADN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407723"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VHH53_05045"
     CDS             <1..209
                     /locus_tag="VHH53_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289557.1"
                     /translation="TKAATEATKAPKATKTPKATKAATKAAKATKKAKKEAKIDKKVT
                     TTASAKVPTKVTAKKAVKSSATKN"
     gene            complement(241..1140)
                     /locus_tag="VHH53_05050"
     CDS             complement(241..1140)
                     /locus_tag="VHH53_05050"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2289558.1"
                     /translation="MPGLIAAGAVVWQLSPSGDVEVALVHRPRYDDWSLPKGKPHSGE
                     ALPLTAVREVGEETGHTVALGARLGRTRYLVRWGQKVAHYWAARPTGGSFRPSEEVDE
                     LRWLPVAKAADLLTHRHDRTLLAGLDGATAITNTVLLVRHGDAGEREEWQGDDDLRPL
                     TMAGRRQAEGLRTLLPLFGATRVYSAPLVRCRQSVEGLAADLGVPIIEEPQLSEEGYL
                     ANPTRGLGRLIEIADEAEGPAVVCSQGGVIPDVVSKLAKKAGLDLADVASRKGSFWGL
                     FFRSEPPAPPVLLAADYYNDAVG"
     gene            complement(1144..>1763)
                     /locus_tag="VHH53_05055"
     CDS             complement(1144..>1763)
                     /locus_tag="VHH53_05055"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334885.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX2289559.1"
                     /translation="TIGADLTDLFNSLTGYARQTRYRSLLVAPYGIRRGIVERIEGEI
                     ARARTGRPSGIRIKVNSLVDEQVIDALYRASLVGVPVDLVVRSICALIPGRQRLSENI
                     RVRSILGRFLEHSRIFHFVGAGEHWIGSADIMHRNLDRRVEVLVRVADQDVTDELDAI
                     LASALDPATRCWILEPDGSWSASPKDGSTVCDHQVQEIRRHRRGE"
BASE COUNT          314 a          619 c          574 g          256 t
ORIGIN      
        1 agacgaaggc ggcgaccgag gcaactaagg cccccaaggc aaccaagacc ccgaaggcca
       61 ctaaggcggc gaccaaggcg gcgaaggcca cgaagaaggc gaaaaaggag gctaagatcg
      121 ataaaaaggt caccacgaca gcttccgcga aggtccctac caaggtcact gcaaagaagg
      181 ctgtcaagtc gtccgccacc aagaactgag ccgatcacag gagcgaccgg gcggagcgtc
      241 tcagcccacc gcgtcgttgt agtagtcggc ggcgagcagg acgggtggtg cggggggctc
      301 gctgcggaag aacagccccc agaagctccc tttgcggctg gccacatcgg ccaggtccag
      361 cccagccttc tttgcgagct tgctgaccac gtcgggaatc actccgccct ggctgcacac
      421 gacggccgga ccctcagctt cgtccgcgat ctcgatcaac cggccgaggc cacgtgtcgg
      481 attggccagg tagccctctt cggacaactg cggttcctcg atgatgggca cccccaggtc
      541 ggcggccagc ccttcaacgc tctggcggca ccgcaccagc ggtgcggaat agacccgcgt
      601 cgctccgaac aggggcaaca gcgtgcgcag gccttcggcc tgccgccggc ccgccatggt
      661 cagcgggcgc aggtcgtcgt cgccctgcca ttcctcgcgc tcacccgcat cgccgtggcg
      721 gaccagcagc acggtgttgg tgatggctgt cgcgccgtcg aggccggcta gcagcgtccg
      781 gtcgtgccgg tgggtaagta ggtcggcggc cttggccacg ggcagccagc gcagctcgtc
      841 gacctcctcg ctgggtcgaa agctgccgcc ggtaggacgc gccgcccagt agtgcgccac
      901 cttctggccc cagcgcacca gatagcgtgt gcggcccagc ctggcaccga gggcaacggt
      961 gtgcccggtc tcctcaccta cttcgcggac ggcggtgagc ggcagcgcct cccctgagtg
     1021 cggcttgcct ttaggaagcg accagtcgtc gtagcggggc cggtgcacca gcgccacctc
     1081 gacgtcgccg gagggcgaca gctgccacac cacggcgccc gcggcgatca gtcccggcac
     1141 cgctcattca cccctgcggt gccggcggat ctcctgcacc tgatggtcgc agaccgtgga
     1201 tccgtccttg ggggaggcac tccaggaccc atccggttcc aatatccagc atcgcgtcgc
     1261 cgggtccaga gcggaggcga gtatcgcatc cagttcgtcg gtcacgtcct gatcggccac
     1321 ccgtaccaac acctccaccc ggcggtccag gttgcggtgc atgatgtccg cgctgccgat
     1381 ccagtgctcg ccggcgccca caaagtgaaa gatccgcgag tgctcgagga accggcccag
     1441 gatcgagcgg acccggatat tctccgagag cctctgacgc ccggggatca gcgcgcagat
     1501 gctgcggacc accaggtcga ccggaacacc caccagcgac gcccggtaca gcgcatcgat
     1561 cacctgctcg tccaccagag agttcacctt gatccggatc ccggacgggc ggcccgttcg
     1621 agcgcgggcg atctcaccct cgatccgctc cacgatgccg cgccggatgc cgtagggcgc
     1681 caccagcaga ctgcggtagc gagtctgccg cgcgtagccg gtcagtgagt tgaacaggtc
     1741 ggtgaggtcg gcaccgatcg tcg
//
LOCUS       DATADN010000383         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_648784, whole genome shotgun sequence.
ACCESSION   DATADN010000383 DATADN010000000
VERSION     DATADN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648784"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VHH53_05060"
     CDS             complement(<1..434)
                     /locus_tag="VHH53_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2289560.1"
                     /translation="MQRLRASYRAIPAGSPVRLAKRTSNLFRSRAATGTPGLDVSGLT
                     GVLQVDPATRTADVAGMCTYENLVAATLPHGLTPLVVPQLKTITLGGAASGLGIEAAS
                     FRNGLPHESVLELDVLTGAGEIVTATQGDDLFAGFPNSYGTLG"
     gene            652..972
                     /locus_tag="VHH53_05065"
     CDS             652..972
                     /locus_tag="VHH53_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX2289561.1"
                     /translation="MNMIMQQAQKMQQQLMSAQAELAEAEVTGQAGGDLVLVTLAGSG
                     EVLSVTIDPKVVDPDDVETLQDLVVGAMADAHQALQDLTQQKMGPLASALGGPGGGLG
                     LPGV"
     gene            1054..1650
                     /gene="recR"
                     /locus_tag="VHH53_05070"
     CDS             1054..1650
                     /gene="recR"
                     /locus_tag="VHH53_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX2289562.1"
                     /translation="MYEGVVQDLIDELGRLPGVGPKSAQRMAFYLLAADPADVTRLQD
                     ALQRVKDGVVFCAVCGTVSEKETCRICADPRRDPTVVCVVEEPKDVLAVERTREFTGR
                     YHVLGGALDPLSGVGPDQLRIRQLLTRIAADDVTEVIIATDPNTEGEATATYLARMLR
                     DFPGLTVTRLASGLPMGGDLEFADELTLGRAMSGRRAV"
BASE COUNT          318 a          597 c          629 g          315 t
ORIGIN      
        1 cccagcgtgc cgtaggagtt gggaaagccg gcgaacagat catcaccttg agttgcggtg
       61 acgatctcac cagcgcccgt gagcacgtcc agctccagga cggactcgtg cggcaagccg
      121 ttgcgaaacg atgccgcctc gatgcccaac ccactcgccg caccgcccag ggtgatcgtc
      181 ttgagctgcg gcacgaccag cggggtgagc ccgtgcggca gggtggcggc gacgaggttt
      241 tcgtaggtgc acatcccggc gacatcagcg gtgcgggtgg ccgggtcgac ctgcaggacc
      301 ccggtaaggc cggagacgtc caggcccgga gtgcctgtcg cagcacgcga ccggaacagg
      361 ttcgacgttc gtttggccag ccgtaccggc gaccccgctg ggatcgcccg gtaactggct
      421 cgcagccgct gcacagcatc gtgatggctg cgctgcgccc cggtgagctg cacgcccttt
      481 acgctacggc caaacagggc tcgaccgcac gatttctctc gcgccgacgt gactaccctg
      541 tgacatcact actctgtgac atagccactt gggccacacg ggtcgtgcgc tgtgccccgc
      601 aaggcccgat cgacgagagg agccgcagtg caacccggtg gagcgcccga tatgaatatg
      661 atcatgcagc aggcgcagaa aatgcagcag cagctgatga gcgcgcaggc agaactcgct
      721 gaggcggagg tcaccggaca ggccggaggt gacctggtct tggtcacact ggccggctcc
      781 ggcgaggttc tcagcgtgac tatcgacccc aaggtcgttg accctgacga cgtggaaacc
      841 ttgcaggacc tcgtggtggg cgcaatggct gacgcccacc aggccttgca ggacctcacc
      901 cagcagaaga tgggtccgct ggccagcgcc ctcggtgggc ctggcggggg tctgggcctg
      961 cctggggtgt gatggcctcc ggcgcgctac gtcccggcaa ggcggaatca tgcccaccga
     1021 cgtactgcgt cccgcggagg cggaatcatg actgtgtacg agggcgtcgt ccaggacctc
     1081 atcgacgagc tggggcggtt acccggtgtc gggccgaaga gcgcgcaacg gatggccttc
     1141 tacctgctgg ctgccgaccc ggcggacgta acgcggctgc aggatgcgct gcagcgggtc
     1201 aaggatgggg tggtcttctg cgctgtctgc ggcacggtct cggagaagga gacgtgccgg
     1261 atctgtgctg atccccgccg cgatcccacg gtggtctgtg tggtcgagga accgaaggat
     1321 gtgctcgcgg tggagcgcac ccgtgagttc accggccgct atcacgtgtt gggtggcgcg
     1381 cttgatcccc tgtccggcgt cggccccgat caactgcgga tccgacagtt gctgacccgg
     1441 atagctgccg acgacgtgac cgaagtgatc atcgcgacgg atccgaacac cgagggcgag
     1501 gccactgcca cctacctggc gcggatgctg cgcgacttcc ccgggctgac ggtaacccga
     1561 ctcgcctccg gcctgccgat gggtggtgac ctggagttcg ccgatgagct caccctcggc
     1621 cgggccatgt ccggacgccg cgccgtctga acgtggtcaa cgctgtctac tgcctgcacg
     1681 tgcatgcgtt agtcgccgtc cggctcgctc tccccgaaat attagaatcc cttggacaag
     1741 tgacacccgc agcgtgggca acgaacaaac tggaccgact gctgcgtgtt tcaaagcgcc
     1801 tgccactctg cattgatcgt gcgacgcgcg tctgcatgcg gcgaagtttg ggttggaac
//
LOCUS       DATADN010000384         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_501791, whole genome shotgun sequence.
ACCESSION   DATADN010000384 DATADN010000000
VERSION     DATADN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501791"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1252)
                     /locus_tag="VHH53_05075"
     CDS             complement(278..1252)
                     /locus_tag="VHH53_05075"
                     /inference="COORDINATES: protein motif:HMM:NF005920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2289563.1"
                     /translation="MTAAQITMERDAGTEADLDAQSPAADLVRVYLNGIGRTALLSAQ
                     QEVELAKRIEAGVFARHVLDTAPRLSPTRRADLTRLVHDGQKAKDHLLEANLRLVVSL
                     AKRYTGRGMPLLDLIQEGNLGLIRAVEKFDYTKGFKFSTYATWWIRQAITRGMADQGR
                     TIRLPVHLVEQVNKLARIKRDLHQRLGREATHQELADESGIAVEKVADLLEHSRDPVS
                     LDMPVGAEEEAPLGDFIEDAEAADAESAVISGLLQDDLRRVLATLEDREQKVIRLRYG
                     LDDGQPHTLDQIGKRFGLSRERVRQIEREVMAKLRHGDRAERLRAYAS"
     gene            complement(1517..>1929)
                     /gene="dtd"
                     /locus_tag="VHH53_05080"
     CDS             complement(1517..>1929)
                     /gene="dtd"
                     /locus_tag="VHH53_05080"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX2289564.1"
                     /translation="SRVTEAAVLVDDEVVGELNRPGLLVLLGVTHSDGSAQAQTMARK
                     LYELRILRDDRSVAATDAGVLVVSQFTLYGTARKGRRPSFTAAARPDQAQLLIEEVIT
                     RLRAHGATVATGRFGAMMLVRSVADGPFTVLLDV"
BASE COUNT          298 a          659 c          623 g          349 t
ORIGIN      
        1 cccgtcgcta gctgtacagc tcgacgcccc gctaggggac cgcaccgtcg tcttggagcg
       61 tcacaccatc ggcccgccac ccggacgcta acgttttgga cggaaggccg ggcccccgac
      121 gagcccgacc ttccggggcg cgcgacagcc tgacgggttg accgccaagt cgcgtggtct
      181 acgtcacatt ctagtcacgt ttccgctgac agtgcctgtc gcagcctgcg ctgtcaatgc
      241 cgccattcga gcggttatgc gctgaagctg aagaagatca gctggcgtag gcccgcagcc
      301 gctccgcacg gtccccgtgg cgcaacttgg ccataacctc gcgctcgatc tgacgaaccc
      361 gctcgcggga cagcccaaag cgcttcccga tctggtccag cgtgtgcggc tgaccgtcgt
      421 caaggccgta gcgcagccgg atgaccttct gctcccggtc ctcgagagtg gccagtaccc
      481 ggcgcaggtc atcctgcagc aacccggaga tcaccgcgga ctcggcgtca gctgcctcgg
      541 catcctcgat gaagtcgccc agcggggcct cctcctcggc accgactggc atgtccaggc
      601 ttaccgggtc tcgcgagtgt tcaagcaggt cggcgacttt ctccaccgcg ataccggatt
      661 cgtcggccag ctcctggtga gtggcctccc gtcccagccg ctggtgcagg tcccgcttga
      721 tgcgggccag cttgttcacc tgctccacaa ggtgcaccgg aagccggata gtgcgtccct
      781 ggtcggccat gccacgggtg atggcctgcc ggatccacca ggtggcgtag gtcgagaact
      841 tgaagccctt ggtgtaatcg aacttctcca cggcacggat cagaccgaga ttcccctcct
      901 gaatcaggtc cagcaacggc attccgcggc cggtgtagcg cttggccagc gacaccacca
      961 gccgtaggtt cgcctccagc aggtggtctt tggccttttg gccgtcatgc accagtcgcg
     1021 tcaggtcggc ccgccgggta ggtgacagcc gcggggcggt gtcaagcacg tgacgggcga
     1081 atacgcctgc ttcgatgcgc ttggcgagct caacctcctg ctgggcgctg agcagcgcgg
     1141 tgcgtccgat accgttgagg tacacccgaa ccagatcggc tgccgggctc tgcgcatcca
     1201 ggtctgcctc ggtaccggcg tcccgttcca tggtgatctg cgcggcggtc atcaggttcc
     1261 ttcctgcgac ggatcctcgt gccggcccgg tgccggtgcg ctcccgagtc ggcgtgcggc
     1321 gctgtacgag ctgccagcga gtacccgcag cgatgactgg tacaacgtcg ggagcccgga
     1381 ttccgttccc gagccctcac tgccacgacg aactagtgct cggtgaagtg ccggattttt
     1441 tcgcaccctc agatacttct caggttggca gccgtttgag accgttgccg cccgggttgg
     1501 tagctcttgg agggcgtcac acgtcgagga ggacggtgaa cgggccgtcg gccacgctgc
     1561 ggacgagcat catcgccccg aaccggccgg tggccacagt ggcgccgtgg gcccgcagcc
     1621 gggtgatgac ctcctcgatc aacaattggg cctgatccgg ccgggcggcc gcggtgaagg
     1681 agggtcgccg gcccttgcgc gccgtcccgt aaagggtgaa ctggctgacc accagcacgc
     1741 cggcgtcggt ggcggcgacc gagcggtcgt cgcgcaggat ccgcagttca tacagcttgc
     1801 gggccatcgt ctgggcttgt gccgacccgt cgctgtgggt gacgcccagc agtaccagca
     1861 ggccaggccg gttcagctca ccgaccactt cgtcatcgac gagcactgct gcctcagtga
     1921 cgcggctta
//
LOCUS       DATADN010000385         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_649053, whole genome shotgun sequence.
ACCESSION   DATADN010000385 DATADN010000000
VERSION     DATADN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649053"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VHH53_05085"
     CDS             <1..908
                     /locus_tag="VHH53_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2289565.1"
                     /translation="VTLLAEGHLLVEDVPGVGKTSLAKALARSIDCTVSRIQFTPDLL
                     PSDVTGVSIFNRQTNDFEFRPGPVFANIVVGDEINRASPKTQSALLECMEEQQVTVDA
                     NTYRLQAPFMVIATQNPIEMEGTYALPEAQRDRFTTRVSIGYPDPNAELAMVDEHAGA
                     DPMSTLQPVSDAAQISSLIETVRRVELSPDIRRYAVELVSATRRLPEIRIGASPRSTL
                     HLVRAARAQAALAGRDFVVPDDLHAVAIPVLAHRLVLTAEAQAGRRSSGDLIRNLIQR
                     VPVPQGALDPTAQWVGGISPARRTR"
     gene            915..>1869
                     /locus_tag="VHH53_05090"
     CDS             915..>1869
                     /locus_tag="VHH53_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2289566.1"
                     /translation="MRGVLSGLTTRGRCLLAAGLAAALCSIVLNERDLLRVSVFVVAL
                     PVLVCVLTAVAQVGLHAERLIMPSRVPVGGRVEVAVHVRSTGRLPTGGLLLQDGVPYA
                     LGGKPRFVIEHLPRHTGTQLRYAVHPTLRGVQQLGPLKATITDPFGLAEFDRELAQTS
                     RLIVVPRVVRLTGLPSGSGLGSGDDGSVRLNAGQGEDDAIVRPYRQGDDLRKVHWRST
                     AKRDEMMVRVEERPWRGGTTILLDHRAVAHRGAGPTASIEWAISFAASVCLHLHRYGQ
                     RIRLVTESGRLLVGDSGDGSHHDNLVLDALAVLQPNPQQEID"
BASE COUNT          254 a          699 c          655 g          261 t
ORIGIN      
        1 tggtcaccct gctggccgag ggccacctgc tggtcgagga cgtacccgga gtgggcaaga
       61 cctcgctggc caaggcgctg gcccggtcga tcgactgcac cgtcagccgg atccagttca
      121 cccccgacct gctgcccagc gacgtcaccg gggtgtcgat cttcaaccgg cagaccaacg
      181 acttcgagtt ccgtcccggc ccggtgttcg ccaacatcgt ggtcggtgac gagatcaacc
      241 gggcctcccc gaagacccag tccgcgctgc tggaatgcat ggaggagcag caggtcaccg
      301 tcgacgccaa cacctaccgg ttgcaggcgc cgttcatggt gatcgccacg cagaacccga
      361 tcgagatgga gggcacctac gccctgcccg aggcgcagcg ggaccggttc accacccggg
      421 tgtcgatcgg ctacccagac ccgaacgccg aactggccat ggtcgacgag catgccggcg
      481 cggacccgat gagcaccctc cagccggtgt ccgacgccgc ccagatcagc agtctgatcg
      541 agaccgtgcg ccgggtggag ctgtcccccg acatccggcg ctacgccgtc gagctggtgt
      601 cagccacccg ccggctgccg gagatccgca tcggcgcctc cccgcgctcc accctgcacc
      661 tggtgcgcgc cgcgcgggcc caggcggcgc tggccggtcg cgacttcgtg gtgcccgacg
      721 acctgcacgc ggtggccatc ccggtgctcg cccaccggct ggtgctcacc gccgaggcgc
      781 aggccggccg ccgctcctcg ggcgacctga tccgcaacct gatccagcgg gtgccggtgc
      841 cccagggcgc gctcgatccg accgcccagt gggtgggcgg gatctcgccg gcccggcgta
      901 cccggtagtc cgggatgcgc ggcgtgctgt ccggtctgac cacccgcggc cggtgcctgc
      961 tggccgccgg gctggccgcc gcgctgtgct cgatcgtgct caacgagcgg gacctgctgc
     1021 gggtgtcggt gttcgtggtg gcgctgcccg tcctggtgtg cgtgctcacc gcggtggccc
     1081 aggtcggact gcacgccgaa cggctgatca tgccgtcgcg ggtgccggtc ggtggccggg
     1141 tggaggtcgc cgtgcacgtg cgcagcaccg gccggctgcc caccggcggt ctgctcctgc
     1201 aggacggcgt gccgtacgca ctgggtggca agccacgctt cgtgatcgag cacctgcccc
     1261 ggcacaccgg cacccagctg cgctacgccg tgcaccccac gctgcgcggc gtgcagcagc
     1321 tcggcccgct caaggcgacg atcaccgacc cgttcgggct ggccgagttc gaccgcgagc
     1381 tggcccagac gtcgcggctg atcgtggtgc ccagggtggt ccggctgacc ggcctgccca
     1441 gcggctccgg cctgggctcc ggcgacgacg ggtcggtacg gctcaacgcc gggcagggcg
     1501 aggacgacgc catcgtgcgg ccgtaccggc agggcgacga cctgcgcaag gtgcactggc
     1561 gctcgaccgc caagcgcgac gagatgatgg ttcgggtcga ggagcggccg tggcgcggtg
     1621 gcaccacgat cctgctcgac caccgcgccg tcgcccaccg cggcgccggc ccgaccgcca
     1681 gcatcgagtg ggcgatctcg ttcgccgcca gcgtgtgcct gcacctgcac cggtacggcc
     1741 agcggatccg gctggtgacc gaaagcggcc ggctgctggt cggcgactcc ggcgacggct
     1801 cgcaccacga caacctcgtg ctcgacgcgc tcgccgtgct gcagcccaac ccgcagcaag
     1861 agatcgact
//
LOCUS       DATADN010000386         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_649150, whole genome shotgun sequence.
ACCESSION   DATADN010000386 DATADN010000000
VERSION     DATADN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649150"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..>1507)
                     /locus_tag="VHH53_05095"
     CDS             complement(416..>1507)
                     /locus_tag="VHH53_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2289567.1"
                     /translation="AKDTGSKYKEHVVSTGPYMFGTNELGKRFTMIRNPHWDPATDPN
                     RQALPDRIRVDLNVNADDVDNRLISGDLDVSIEGSGVGPSAQGRILADQGLRASTDSA
                     LFARLWFTALNSDVAPLDNIHCRKAVLYAADRTGYQRAYGGATGGEIATNLLPPVIPG
                     AERFDLYPSPNNEGDIAKARDELAQCGQPNGFTTAISYRAERPKEKATAEALQQSLAR
                     AGINLEIKPYPLTDYLRLYAGKPDFAKSNSLGLIVYGWGADWPDGYGFLSQLVDSRVI
                     RATGGNSNLGIKDPAIDQLLDQALVTTDTAARERIWVDIDRKVMENAFVLPGVWAKGL
                     LYRPPNLTNVFITDGFQMYDYLALGTTRK"
BASE COUNT          243 a          456 c          522 g          286 t
ORIGIN      
        1 cgcacctttg caaggcggta ctcgactctc cacaggagcc tgcacccaac caagaaatgc
       61 ccttgccggg cgggcaggcc tccagtatca ccagccacaa gaactgctgc ggctgggtca
      121 ggtgggcagg ggtgtggcat caagccccag cgtgcaagat cgactcctcg ggccgtcgcc
      181 ggccgtcgaa cgtcgaaaag cgttgagaaa cctggagaag tagcgagagc gttcctcctg
      241 gttaactccg ctgcgtcctc ccattggcgc agatggcgaa gtggggggcg gacgagctgg
      301 cctgtaagtc ggctcctggt tccggaggag gccgcggtcc aactggtcgt cggtgcagcg
      361 aactgccgac gagtttggga catacgcaaa tgatgtagat gcccgctgtg ctgcgttatt
      421 tccgcgtggt gcccagtgcc aggtagtcat acatctggaa gccgtcggtg atgaacacgt
      481 tggtcaggtt cggcggccgg tacagcaggc ccttggccca gacgccgggt aggacgaaag
      541 cgttttccat caccttgcgg tcgatgtcga cccagatccg ttcccgcgcc gcagtgtcgg
      601 tagtcaccag cgcctggtcg agcaactggt cgatcgccgg gtccttgatt cccaggttcg
      661 agttgcctcc ggtggcccgg atgacccggc tgtcgacgag ctgggacagg aacccgtagc
      721 catcaggcca gtccgcgccc cagccgtaga cgatcaggcc cagactgttg gacttggcga
      781 agtccggctt gccggcgtac aggcgcaggt agtcggtcag cgggtacggc ttaatttcca
      841 ggttgatccc ggcccgcgcc agtgactgct gcagtgcttc ggcggtggcc ttctccttgg
      901 gccgctcggc gcggtaggag atggcagtgg tgaagccgtt gggctggccg cactgggcga
      961 gctcgtcgcg ggccttggcg atgtcgccct cgttgttcgg cgacgggtag agatcgaatc
     1021 tttccgcccc ggggatcacc ggcggcagca ggttcgtggc gatctcgccc cctgtcgctc
     1081 cgccgtaggc gcgctggtaa ccggtgcggt cggcggcata cagcaccgcc ttgcggcagt
     1141 ggatgttgtc cagcggcgcg acatcggaat tgagcgcggt gaaccacagc cgggcgaaca
     1201 gcgcggagtc ggtgctcgcg cgcaggccct ggtcggcgag gattcgacct tgagccgacg
     1261 gcccaacccc ggagccctcg atcgagacgt caagatcacc ggagatgagc cggttgtcga
     1321 catcgtcggc gttgacgttc aggtcgactc tgatccggtc gggcagcgcc tggcggttcg
     1381 ggtcggtggc cgggtcccaa tgggggttgc ggatcatcgt gaaacgcttg cccagctcgt
     1441 tggttccgaa catgtagggg ccggtcgaga cgacgtgctc cttgtacttc gatccggtgt
     1501 ccttggc
//
LOCUS       DATADN010000387         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_649226, whole genome shotgun sequence.
ACCESSION   DATADN010000387 DATADN010000000
VERSION     DATADN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649226"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..589
                     /locus_tag="VHH53_05100"
     CDS             128..589
                     /locus_tag="VHH53_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2289568.1"
                     /translation="MSVSHAALITGCSSGIARATALRLHAAGLPVYATARHVDDLSDL
                     VAKDVTTLALDLTDAESMDVAVKRISAEHGAVGILVNNAGYAELGTIEEIALDDARVQ
                     LEIKRSQTLTPPRCGQPSRLCWRCMAALVTSWRRNSTRSGLLRSATGSARA"
     gene            526..1416
                     /locus_tag="VHH53_05105"
     CDS             526..1416
                     /locus_tag="VHH53_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX2289569.1"
                     /translation="MAQELDAKRAAAVSHGLSQSLMAGITVLRNGGSALDAVVAAVLV
                     LEDDEQFNAGRGAVLTAGGGVEHDAAVADGAMRRAGAVTCVSGIRNPVRAARAVMERT
                     PHVVLSGAGALELARREGLALVPAGWFVTARRQEQLSKVLRGDRAESEVTGETVGAVA
                     LDVEGHLAAATSTGGSAGQLVGRIGDAPLIGAGTWADDRSCAVSATGDGEAFILSVFA
                     HEVDALVRLAGLDLGAACQRALHEVGARAGTGGCIAVGPDATVAMPFTSAGMFRGWID
                     ATGNRRIGMFTEDLTELRGR"
BASE COUNT          254 a          491 c          572 g          270 t
ORIGIN      
        1 acgatgctgc ggatcctcgc tcaccaacca tcgctggcgg ccatccggcc gggcatctca
       61 tcgagccacc agggcgcacc acaggccgct tgatatggcc acgaccagat cgaagggaga
      121 caccgacatg tcggtttccc acgcagccct aatcaccggt tgctcctccg gtatcgcccg
      181 tgccaccgcg ctacggctcc acgcagccgg cctcccggta tacgccacag cccgacacgt
      241 tgacgatttg tctgatctgg tagccaagga cgtcacgacg ttggcgctcg acctcaccga
      301 cgcggagtcg atggacgtcg cggtcaagcg catcagcgcc gagcatggcg cggtcggcat
      361 cctggttaac aacgccggct acgccgagtt gggcaccatc gaagagatcg cgctcgatga
      421 cgctcgggtg cagctggaaa tcaaacgatc tcaaaccctg acacctccgc ggtgtggcca
      481 gccatcgcgc ctgtgttggc ggtgcatggc ggcgctggtc acatcgtggc gcaggaactc
      541 gacgcgaagc gggctgctgc ggtcagccac gggctcagcc agagcttgat ggcaggcata
      601 actgtgttgc gcaacggtgg gtcagcgctc gacgcggtgg ttgctgcggt actagttctc
      661 gaggacgacg agcagttcaa tgccggcagg ggtgctgtcc tcacggctgg cggcggcgtg
      721 gaacatgatg cagcggtggc tgacggggcg atgcgtcgcg ctggagccgt gacctgtgtg
      781 agtggtattc gcaatccggt gcgtgcggcg cgggcggtga tggaacggac gccacatgta
      841 gtgctcagcg gagccggagc gctggaattg gcccgtcggg aaggcctggc gttggtgccg
      901 gccggttggt tcgtgaccgc tcgccgccag gagcagctga gcaaggtgct gcgcggcgac
      961 cgggcggaat ccgaggtgac gggcgagacg gtaggtgccg tcgccctgga cgtcgaggga
     1021 cacctggcgg cggcgacctc gactggaggc agcgctggcc agctcgttgg ccggatcggg
     1081 gacgccccac tcatcggcgc tggcacctgg gccgatgacc gatcctgcgc ggtctcggcg
     1141 actggcgacg gtgaggcgtt catcctcagc gtcttcgctc atgaggtgga cgcgctcgtg
     1201 cggctggccg gtctggactt gggcgcggcc tgccaacggg cgttacatga ggttggggct
     1261 cgggccggaa ccgggggatg catcgccgtg ggaccggacg ccacggtggc tatgccattc
     1321 accagcgcgg gcatgttccg cggctggatc gacgcgaccg gcaatcgccg gatcgggatg
     1381 ttcaccgagg acctcacaga acttcgtggt cgctgaagag cttgagtggg gctctggcga
     1441 gcagagtgga ctccgttacg gcccccacac cgtgacccgt tgggtgaacg gccggcccgg
     1501 acggtgtctc gtgtcctggc gcgccacaag gtgccgcggt tgtgcgtgct ggatcccctc
     1561 actggcgagg tgatcaaagc gtcgtgc
//
LOCUS       DATADN010000388         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1241162, whole genome shotgun sequence.
ACCESSION   DATADN010000388 DATADN010000000
VERSION     DATADN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241162"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1645)
                     /locus_tag="VHH53_05110"
     CDS             complement(<1..1645)
                     /locus_tag="VHH53_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2289570.1"
                     /translation="MANLINFEAVSKSFGIRPLLSGISLGVAEGDRIGVVGLNGGGKT
                     TLLEVLAGIEAPDAGRVSRTRDLRTAVVTQRTELPEQVTVRQAVVDPLGVTAEHEWAG
                     DARMRGMLEGLGIVSLGLDRPVAELSGGERRRVALAAALVGELDLLILDEPTNHLDVE
                     GVRWLAEHLLARRCAAVVVTHDRWFLDTVCTRTWEVVDGRVDGYDGGYADWTYARAQR
                     TRLAAAAEDRRRNLARKELAWLRRGPPARSSKPRYRIEAAEALIADVPAPRDNVELLT
                     FARRRLGRTVLELEDVTVAVGSRKLLDAQKWRIGPGERIGLVGVNGSGKTTLLRLLAG
                     QREPDTGRRVQGSTVRLAHLTQELENLPESLRVLEAVQEVANRVALGRQDLSASQLAE
                     RLGFPASRQWTPVGDLSGGERRRLQLTRLLMAEPNVLLLDEPTNDLDIDTLQQLEDLL
                     DGWPGSLVVVSHDRYLVERVCDTVVALFGDGRITQLPGGIEEYLQRWDAAAGIEAAVA
                     TGTAQTYPAPPEAFTGAAQQRMARKELARPANRSSCRSSR"
     gene            complement(1645..>1767)
                     /locus_tag="VHH53_05115"
     CDS             complement(1645..>1767)
                     /locus_tag="VHH53_05115"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156090.1"
                     /note="catalyzes the phosphorylation of
                     4-diphosphocytidyl-2-C-methyl-D-erythritol in the
                     nonmevalonate pathway of isoprenoid biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX2289571.1"
                     /translation="AELAGAGVCRTVRVASGPVPGARVVEDPRPVPPPEPLAHA"
BASE COUNT          279 a          666 c          563 g          259 t
ORIGIN      
        1 ctcggctgga acggcagctg gagcggttgg ccggccgagc caactccttg cgcgccatcc
       61 gctgttgcgc agcgccggta aatgcctccg gtggtgccgg atacgtctgc gcggtgccgg
      121 tggcaacagc agcctcgatt cccgctgctg catcccaacg ttgcaggtac tcctcgatgc
      181 cgccgggtag ctgggtgatc cgtccgtcac cgaacagcgc caccacggtg tcacacaccc
      241 gttcgacaag gtagcggtcg tgcgagacca ccaccagcga gccgggccag ccgtccagca
      301 gatcttccag ttgctgcagg gtgtcgatgt ccagatcgtt ggtgggctca tcgagcagca
      361 acacgttcgg ctccgccatc aacagccggg tcagctgcag cctgcgccgt tccccgccgg
      421 acagatcccc caccggagtc cactgccgcg acgccgggaa gccgagccgc tcggccagct
      481 gcgacgccga caggtcttga cggcccaacg caacccggtt agcgacctcc tgcaccgctt
      541 ctaagactcg tagcgactcg ggcaagttct ctaattcctg tgtcaggtgc gctagccgga
      601 cagtcgagcc ctgcacccgg cggccggtgt ctggctcacg ctgcccggcg agcagccgca
      661 acaaggtggt cttgcccgac ccattgaccc cgaccaaccc gatgcgctcc ccagggccga
      721 tccgccactt ctgagcgtcc agcagcttgc ggctgcccac ggccaccgtg acgtcctcga
      781 gttccagcac ggtgcggccc agtcggcgcc gggcgaaggt cagcaactcg acgttgtccc
      841 gcggcgcggg aacgtcagcg atcagagcct cggcggcctc gatccggtaa cgcggcttgg
      901 agctgcgcgc aggcggtccg cggcgtagcc aggcgagttc cttgcgagcc aggttccgtc
      961 gccggtcctc agctgccgcg gccaaccggg ttcgctgcgc ccgtgcgtag gtccagtccg
     1021 cgtaaccccc gtcgtagccg tccacccggc cgtcaacgac ctcccaggtg cgggtgcaga
     1081 cggtgtccag gaaccaccgg tcgtgggtga ccaccaccgc cgcacagcgc cgcgccagca
     1141 ggtgctcggc aagccaccgc acaccctcga cgtcgaggtg gttggttggc tcatcaagaa
     1201 tcagcagatc aagctcacct accagtgcgg cggccagcgc gacccggcgc cgctccccac
     1261 cggagagctc ggccaccggc cggtccagcc cgagcgacac gatgcccagc ccctcgagca
     1321 tcccgcgcat ccgggcgtcg ccggcccact cgtgctcagc ggtgaccccc aacggatcca
     1381 ccaccgcctg gcgcacggtg acctgctcgg gcagctctgt gcgctgggtc acgaccgcgg
     1441 tacgcaagtc cctggtccgg cttacccgcc cggcgtccgg tgcttcgatt ccggcgagca
     1501 cctcgagcag ggtggtcttg ccaccaccgt tgagcccgac gaccccgatc cggtcgccct
     1561 cggccacacc cagcgagata cccgacagca gcgggcgaat cccgaaggac ttcgacacgg
     1621 cctcgaaatt gatgaggttc gccatcatgc gtgcgcgaga ggctcggggg gcggcactgg
     1681 gcgcgggtcc tccaccacac gtgcgccggg caccgggccg ctggcgaccc gaacggtgcg
     1741 gcaaaccccg gcgccggcca gctcggc
//
LOCUS       DATADN010000389         3134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_958287, whole genome shotgun sequence.
ACCESSION   DATADN010000389 DATADN010000000
VERSION     DATADN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958287"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="VHH53_05120"
     CDS             complement(<1..148)
                     /locus_tag="VHH53_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX2289572.1"
                     /translation="MTVVQDSVLPSRGGTPDRFTVRLDNFEGPFDLLLQLISQQQMDV
                     TEVAL"
     gene            complement(145..1053)
                     /locus_tag="VHH53_05125"
     CDS             complement(145..1053)
                     /locus_tag="VHH53_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2289573.1"
                     /translation="MSTWASAQQPLAVPTPPISYDDGVVDDGIDPAGRPRRTVPQPPP
                     LDRHGPARVVAICNQKGGVGKTTSTINLGAALAEYGRRVLLVDFDPQGALSVGLGVAS
                     HQLDRTIYNVIMEQGVPVQDVVQTTSVPGMDLLPSNIDLSAAEVQLVTEVGREQTLGR
                     TLRPLLPHYDFILVDCQPSLGLLTINALACADGVVIPLECEFFSLRGVALLIDTIDKV
                     RERLNPKLAITGILATMYDPRTLHAREVMARVVEAFGDTVFDAVINRTVRFPETTVAG
                     EPITRWAPSSAGANAYRTLAREVIAR"
     gene            complement(1123..2025)
                     /gene="xerD"
                     /locus_tag="VHH53_05130"
     CDS             complement(1123..2025)
                     /gene="xerD"
                     /locus_tag="VHH53_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX2289574.1"
                     /translation="MGAYLDHLAVERGTSPNTLDSYARDLRRYGAYLDSLGVAGLAEV
                     REEQVNGFLAALREGAGGHRPLAPSSAARALVAARGLHRFAMWEGLVPTDVAADVHPP
                     SPPKRLPKALGVHEVLRLLEVFPAEGGPRALRDRALLEMLYSTGARISEAVGLDRDDI
                     DAPARTVLLRGKGGKQRLVPVGRPALAALDAYLVRGRPALAARGRGVSAVFLNARGAR
                     LSRQSAWAVLREAAAQTGITAAVSPHTLRHSFATHLLEGGADVRVVQELLGHASVTTT
                     QVYTLVTVDTLREVYATAHPRARG"
     gene            complement(2132..>3134)
                     /gene="ald"
                     /locus_tag="VHH53_05135"
     CDS             complement(2132..>3134)
                     /gene="ald"
                     /locus_tag="VHH53_05135"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX2289575.1"
                     /translation="AGAGCAFPDETYLAAGARIVPLADDVWAQAQLVLKVKEPVPEEY
                     HRMRKGQILFTYLHLAASAECTAALIDAGTDAIAYETVQLPDSSLPLLAPMSEVAGRM
                     APQVGAHCLERMQGGRGVLLGGVSGVPAGKVAVIGAGVVGMNAATIALGMQAEVVVLD
                     ANINRLREIDFVYRGHLQTIASNAYEVEKACLWADLVIGAVLVPGAKAPTLVSHDLVG
                     RMRPGSVLVDIAIDQGGCFADSRPTTHADPTFTVQESVFYCVANMPGAVPHTSTYALT
                     NVTLPYVCALADKGLRRAVDDDPALARGVNVLAGSVVLAEVASAHDLDYVPLAEALG"
BASE COUNT          518 a         1113 c         1039 g          464 t
ORIGIN      
        1 gcaaagcgac ctcggtcaca tccatctgct gctgggagat cagctgcaac agcaggtcga
       61 acggcccctc gaagttgtcc aaccgcacgg tgaagcggtc gggcgtgccc ccacgcgatg
      121 gcaacaccga atcctgtacc accgtcatcg ggcgatcacc tcacgggcca gggtgcgata
      181 ggcatttgcc ccggcggaac tcggtgccca ccgagtgatc ggctcgccgg cgactgtggt
      241 ctccgggaac ctgaccgttc ggttgatcac ggcgtcgaac acggtgtcgc cgaaagcttc
      301 caccacacgg gccatcacct cacgggcgtg cagcgtacgc gggtcgtaca tggtggcgag
      361 gatcccggtt atcgcgagct tggggttgag ccgctcgcgc accttgtcga tggtgtcgat
      421 gagcagcgcc accccacgca gactgaagaa ctcgcactcc agcgggatca ccacgccatc
      481 ggcgcacgcc agcgcattga tggtgagcag gcccagcgac ggctggcagt cgaccaggat
      541 gaagtcatag tggggtaaca gcggccgcag ggttcggccc agcgtctgtt cccggcccac
      601 ctcggtcacc agctgcactt ccgcggcgga cagatcgatg ttgctcggca gcaggtccat
      661 acccggcacc gaggtggtct gcaccacgtc ctgtaccggg acaccctgtt ccatgatcac
      721 gttgtagatg gtgcggtcca gttggtggga ggccactccg agcccgaccg acagcgcccc
      781 ctgagggtcg aagtcgacca gcagcacccg gcggccgtat tcggccagcg cagcccccag
      841 gttgatcgtc gaggtggtct tacccacgcc gcccttctgg ttgcagatcg ccaccacccg
      901 ggccggtccg tgccggtcca gcgggggtgg ctgcggcacc gtacggcgcg gacgaccggc
      961 aggatcgatc ccgtcgtcga cgaccccgtc gtcatacgag atcggcgggg tcggcacagc
     1021 cagcggctgc tgcgcggacg cccaggtcga catggaaccc cttccagcgc ggtgcaatcc
     1081 tcggggagac tattccgctg ccgaaagcgc cttcaacgcg cctcagccac gagcccgcgg
     1141 atgtgcggtt gcatagacct cacgcagggt gtccaccgtc accagcgtgt aaacctgtgt
     1201 ggtggtcacc gaggcgtgcc cgagcagttc ctgcaccact cgcacgtccg caccgccctc
     1261 cagcaggtgg gtggcgaagg aatgccgcag ggtgtgcggt gacaccgcag cggtgatccc
     1321 ggtctgggcg gcagcctccc gcagcaccgc ccaggcactc tgccgggaca gccgggcacc
     1381 gcgggcgttg aggaacaccg cggaaacgcc ccggccgcgg gcggccaggg ccgggcggcc
     1441 gcgaaccagg taggcatcca gcgcggcgag cgccgggcga ccgaccggca ccagccgctg
     1501 cttgccaccc ttgccgcgca gcaacacggt ccgagccggg gcgtcaatgt cgtcgcgatc
     1561 cagcccgact gcctcggaga tccgcgcgcc ggtggaatac agcatttcca gcagcgcccg
     1621 gtcgcgcagc gcacggggtc ccccctcggc ggggaacacc tccagcagcc gcagtacctc
     1681 gtgcaccccc aacgctttgg gcagccgctt cggcgggctc ggggggtgca cgtcggccgc
     1741 gacgtcggtt ggcaccaggc cctcccacat ggcgaaccgg tgcaacccgc gagccgcgac
     1801 cagggcacgc gccgccgagg aaggggccag cggccggtgc ccaccggcgc cttctcgcag
     1861 cgccgcaagg aacccgttca cctgttcctc acgcacctcg gcgagcccgg cgacaccgag
     1921 tgaatccagg taggcgccgt agcggcgcag gtcgcgagcg taggaatcca gcgtgttggg
     1981 cgacgtgccg cgttccaccg cgaggtggtc caggtaagca cccatcaccg ccgccaagtc
     2041 cttcacccgc caagtaaaac cttttggcgc ttcccaacgg gtgagacctg ccgaaggatt
     2101 ccatttggcg ggggctgcgg caggccagcg ctcacccgag tgcctcagct agcggcacgt
     2161 aatcgaggtc gtgtgcgctg gcgacctcgg cgagtaccac ggagcccgcc agcacattga
     2221 ctccgcgggc cagtgccggg tcgtcgtcca ccgcccgccg cagtcccttg tcggccagcg
     2281 cgcacacgta gggcagcgtg acgttggtca acgcatatgt ggaggtgtgc ggtaccgcgc
     2341 cgggcatgtt ggccacgcag tagaacaccg actcctgcac ggtgaaggtg ggatcggcgt
     2401 gcgtggtggg ccgggaatcg gcgaagcagc caccctggtc gatggcgatg tccaccagca
     2461 ccgatcccgg gcgcatccga ccgaccaggt catgactgac cagggtgggc gccttggcgc
     2521 cgggcaccag taccgccccg atcaccaggt ccgcccagag gcaggccttc tccacctcgt
     2581 aagcattcga cgcgatagtc tgcaggtggc cgcggtagac gaagtcgatc tcccgtagcc
     2641 ggttgatgtt ggcgtcgagc accaccactt cggcctgcat ccccagcgcg atggtcgcgg
     2701 cgttcatccc cacgaccccg gcgccgatca ccgccacctt gccggccggc acgccggaga
     2761 caccgccgag caacaccccg cgcccgccct gcatgcgctc caggcagtgc gcccccacct
     2821 ggggggccat ccgtccggcg acctcgctca tcggggccag cagcggcagt gagctgtcag
     2881 gcagttgcac cgtctcatag gcgatggcgt cggttccggc gtcgatcagt gctgccgtgc
     2941 actccgccga cgcggccagg tgcaggtagg tgaacagaat ttggcccttg cgcatccggt
     3001 ggtattcctc gggcaccggt tccttcacct tgagcaccag ttgcgcctgc gcccacacgt
     3061 cgtcggccaa cgggacgatt cgggcgcccg ctgccagata ggtctcgtcg gggaatgcgc
     3121 agccggcacc ggcg
//
LOCUS       DATADN010000390         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_32344, whole genome shotgun sequence.
ACCESSION   DATADN010000390 DATADN010000000
VERSION     DATADN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32344"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VHH53_05140"
     CDS             complement(<1..432)
                     /locus_tag="VHH53_05140"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2289576.1"
                     /translation="MVDIDSVRSWQGRTLVDRDGDRVGTIEAIYVDDQSGQPEWALVN
                     TGLFGTKSTFVPLTQAKPAGDQVQVPYQKQLVKDAPTMDPDGHLSEAEEQQLWRHYGL
                     EYDSGYAADRDGDGVYDRVEDTAVGRDTSGPTTDDAMTRSEE"
     gene            complement(769..1746)
                     /locus_tag="VHH53_05145"
     CDS             complement(769..1746)
                     /locus_tag="VHH53_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2289577.1"
                     /translation="MAEGGTSPEDRSRAGTAERPTQLPGGSWWAAVKRTVREFQVDNL
                     KDWAAALTYYSVLSIFPALVVLISLIDLAGRSTIQTLLDNLGQVAPGSVNQILEGAIR
                     SLQQTRGSAGVLALAGLAAALWSASNYIAAFMRASNAIYDVPEGRPVWKTLPIRIAVT
                     VLVMVLLAASAVAVVATGGLADRLGRLLGLGSAVVTIWDLVKWPVLLLVVSFMFALLY
                     WASPNAKQGFRWVTPGGILAVVVWVAASVAFAIYVANFNSYNKTYGSLASVIIFLVWL
                     WLSNTAILLGAELNAELERSRAIAAGHPPDEEPYMELRDTRKLRERDNL"
BASE COUNT          308 a          670 c          656 g          311 t
ORIGIN      
        1 ctcctcggag cgggtcatgg cgtcgtcggt ggtcggcccg gaggtgtccc gacccacggc
       61 ggtgtcctcg acgcggtcgt agacgccgtc gccgtcccgg tcagcggcgt acccgctgtc
      121 gtactccagg ccgtagtggc gccacagctg ctgctcctcg gcctcggaca gatgcccgtc
      181 ggggtccatg gtgggggcgt ccttgaccag ctgcttctgg tagggaacct ggacctggtc
      241 gccagccggc ttggcctggg tcagcggcac aaaggttgac ttggtgccga acaggccggt
      301 gttgaccagg gcccactccg gctggccgga ctggtcatcg acgtagatgg cctcgatggt
      361 gccgacccgg tcgccgtccc ggtcgaccag ggtgcggccc tgccagctgc ggacgctgtc
      421 gatatccacc atcgctgcct ccttgcttgt tccggtggga tgagctcagg cgaggtgggg
      481 gcgggcacct ccgcggactg accgccgggc acccacccca aacgagaatc accctctcgg
      541 cgtaaccggc gtctgatctg agcgcctctg cggttgtaga tgggtacgca gcccattcct
      601 tgcctggcct acccccgcgg ccaacatgac gcggtcaccg gatgtaggtc cagcaaacca
      661 gccgtatcgc gtctcaaccc gatgctcttc atcgggtcat tcatccacct ggcgacctgg
      721 ccgcctggct tgccgctgtc gtgaggtcag tggacgacgg cctgcgggct acaggttgtc
      781 ccgctcccgg agctttctgg tgtcacgcag ctccatgtag ggctcctcgt cgggcgggtg
      841 gccggcggcg atggcccggc tgcgttccag ctcggcgttc agttcggccc cgagcaggat
      901 ggcggtgttg gacagccaca gccacaccag gaagatgatc acactggcca ggctgccgta
      961 ggtcttgttg taggaattga agttggccac atagatggcg aaggccaccg aggcggccac
     1021 ccagaccacc acggccagga tcccgccggg ggtgacccag cggaacccct gtttggcgtt
     1081 gggggacgcc cagtacagca gcgcgaacat gaagctgacc accagcaaca gcaccggcca
     1141 cttgaccagg tcccagatgg tgaccaccgc gctgcccagg cccagcagcc ggccaaggcg
     1201 gtcggccagg ccaccggtgg ccaccaccgc caccgcggag gccgccagca gcaccatgac
     1261 cagcaccgtg accgcgatcc ggatgggcag ggtcttccag accggccggc cctcgggcac
     1321 gtcatagatg gcgttggagg cccgcatgaa cgcggcgatg tagttggacg ctgaccacag
     1381 tgctgccgcc aggccggcca gggccagcac cccggccgac ccgcgggtct gctggaggct
     1441 gcggatggcg ccttccagga tctggttgac cgagccgggg gcgacctggc ccaggttgtc
     1501 cagcagggtc tggatggtgg agcgtccggc caggtcgatc agggagatca ggaccaccag
     1561 ggccgggaag atggacagca cgctgtagta ggtgagcgcc gccgcccagt ccttgaggtt
     1621 gtcgacctgg aactcccgca cggtccgttt gaccgccgcc caccacgagc cgccaggcag
     1681 ctgcgtcggc cgctccgccg taccagcgcg cgaccggtcc tcggggctgg tcccgccctc
     1741 agccatcgga tttccacctc cctaccgaac gtgcgcggcg cagccccgac gcagctccgt
     1801 tgccaggtcg tcaacatcag gaactgcggg ggcagttcat ccgacccgga tacctcacta
     1861 caacctgccg aaccgcggag cagggccttc aggcccaacg tcgctgtctg ccgagtggcg
     1921 agaacggtag ggaggcatcg gtccg
//
LOCUS       DATADN010000391         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_958375, whole genome shotgun sequence.
ACCESSION   DATADN010000391 DATADN010000000
VERSION     DATADN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958375"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2211)
                     /locus_tag="VHH53_05150"
     CDS             complement(<1..>2211)
                     /locus_tag="VHH53_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HEX2289578.1"
                     /translation="TPASAVVLLRALAEAGYDLDDLDVAELDGDTLIHQLIAAGGHDV
                     EWLTEEQMASSAIRVPVTQYAQWFAQLPAALRESVVAAWGEPPGELYVSEGTLYVGSL
                     QFGNVVLFIQPPRGFGENPVAIYHDPELAPSHQYLAAYRWLEQDFGAHAVVHLGKHGN
                     LEWLPGKGLGLAADDAPDAVLGNLPLVYPFIVNDPGEGTQAKRRAHATIVDHLIPPMA
                     RADSYGDLARLEQLLDEYATVAALDPDKLPTLRGQIWTLIQSAQLHRDLGAEAAPGEE
                     DFDDFVLHLDGYLCEIKDVQIRDGLHVLGRAPEGDELVNDVLAVLRARQVFGSGAALP
                     GLRAVIAECIGLDEQGSLADVDRLEALARTLVHGVHAAGWDVSKVPAVVAELLGKPDA
                     GVISVLRFGCTELVPRLAGTPAEITNVLRALDGRFVEPGPSGSPTRGLISVLPTGRNF
                     YSVDPKAIPSRNAFEVGAALADSLLARHRADTGVWPASVGLTVWGTSAMRTQGDDIAE
                     VLALLGVRPSWDDASRRVTGLEVVGLAELGRPRIDVVLRISGFFRDAFPHVVALIDDA
                     IHTVAQLEELDQDNYVAAHYRADLATHGDDRRARTRIFGSKPGAYGAGLLPLIDSRQW
                     RTDADLAEVYATWGGYAYGRGLDGAAARGDMEHAFRRIAVAAKNVDTREHDIADSDDY
                     FQYHGGMVAMVRSLTGSDPAAYVGDSAVPHAVKTRSLAEETRRVFRSRVVNPRW"
BASE COUNT          371 a          772 c          755 g          313 t
ORIGIN      
        1 atccaccgcg ggttgaccac ccgggaacga aagacccggc gggtctcctc ggccagcgac
       61 cgggtcttca cggcatgggg caccgcggag tccccgacgt aggctgccgg gtcgctgcca
      121 gtcaaggagc gcaccatggc gaccatcccg ccgtggtact ggaagtagtc gtcggaatcg
      181 gcgatgtcat gctcacgggt gtcgacgttc ttggcagcca ccgcgatacg gcgaaaagcg
      241 tgctccatgt cgccacgcgc cgcggcaccg tccaggcccc gcccataggc gtaaccaccc
      301 caggtggcgt agacctcggc gaggtcggcg tcggtgcgcc actgccggct gtcgatcagt
      361 ggcagcagtc cggcgccgta agcgcccggt ttggaaccga agatccgggt acgcgctcgc
      421 cggtcgtcac cgtgcgtggc gagatcggcg cggtagtgcg cggcgacgta attgtcctgg
      481 tcaagctcct ccagctgcgc aacggtgtga atcgcatcat cgatcagagc gaccacgtgg
      541 gggaaagcgt cccggaagaa accggagatg cgcagcacga cgtcgatgcg gggcctgccg
      601 agctcggcca gaccgaccac ctccaggccg gtgacccggc gcgaggcgtc gtcccagctc
      661 gggcggacac cgagcagcgc cagtacctcc gcgatatcgt caccctgcgt gcgcatcgca
      721 gacgttcccc agacggtaag gcccaccgag gcgggccaga caccggtgtc agcgcggtgc
      781 cgggcgagca gcgaatcggc cagcgcggcg cccacctcga aggcgttgcg cgacgggatg
      841 gccttggggt ccaccgagta gaagttgcgc ccggtgggca gcacggagat caacccccgg
      901 gtgggtgacc cggacggtcc gggctcgacg aaccggccgt ccagggcgcg cagcacgttg
      961 gtgatctcag cgggagtacc ggcgagccgg ggcaccagct cggtgcagcc gaatcgcagt
     1021 accgagatga ccccggcgtc cggctttccg agcagctcag cgaccacggc cggcaccttc
     1081 gacacatccc agccagccgc gtggacaccg tgcaccagcg tgcgagccag cgcctccagg
     1141 cggtcgacat cggccagcga cccctgctcg tccagaccga tgcactcagc gatgaccgcg
     1201 cgcaatccgg gcagtgccgc accggagccg aacacttggc gggcgcgcag cactgcgagc
     1261 acgtcgttga ccagctcatc gccctcaggc gcgcggccca gcacgtgcag accgtcgcgg
     1321 atctgcacgt ccttgatctc gcagaggtac ccgtcgaggt gcagcacgaa gtcgtcgaag
     1381 tcctcctccc cgggcgcggc ctcggcaccc agatcgcggt gcagctgggc gctctggatc
     1441 agcgtccaga tctgcccgcg cagcgtgggg agcttgtccg ggtccagcgc cgccaccgtg
     1501 gcgtactcgt cgagcagctg ctcaaggcgc gcgaggtcac cgtagctgtc ggcccgggcc
     1561 atcggcggga tcaagtgatc gacgatggtg gcgtgcgccc ggcgcttggc ctgggtgccc
     1621 tcaccggggt cgttgacgat gaacgggtac accagcggca ggttccccag cacggcgtcc
     1681 ggggcatcat cagcggccag tcccagcccc ttgccgggca gccattccaa gttgccgtgc
     1741 ttgcccaggt gcaccacggc gtgcgccccg aaatcctgtt ccagccagcg gtaggcggcc
     1801 aggtactgat gcgacggcgc aagctcgggg tcgtgataga tcgccaccgg gttctcgccg
     1861 aatccgcgcg gcggctggat gaacaacacc acgttgccga actgcaggga gccgacgtag
     1921 agggtcccct cagacacata cagctcgccc ggaggctcac cccaggccgc tacgacggac
     1981 tcgcgtagtg ccgcgggcag ctgcgcgaac cactgggcgt actgcgtgac tggcacccgg
     2041 attgctgagc tcgccatctg ttcctcggtg agccactcga catcgtgccc acccgcggcg
     2101 atcagctggt ggatcagggt gtcaccgtcc agctcggcaa catccaggtc gtcgaggtca
     2161 tagcccgcct cggcgagcgc gcgcagtagc accacggccg acgccggggt g
//
LOCUS       DATADN010000392         2476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_649534, whole genome shotgun sequence.
ACCESSION   DATADN010000392 DATADN010000000
VERSION     DATADN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649534"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VHH53_05155"
     CDS             complement(<1..487)
                     /locus_tag="VHH53_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2289579.1"
                     /translation="MDVVVSGSSGLIGTALKAALETAGHRVIPLSRSKGNAGALHWDP
                     EGGDIDAGGLEGVHAVVHLAGEGIGNKRWNEEQKAKIRNSRVRGTALLAETLAKLARP
                     PRVLLSGSGVGFYGNRGDEVLTETSRPGGGFLAELCTAWEAATAPATEAGIRVCHLRT
                     GI"
     gene            678..750
                     /locus_tag="VHH53_05160"
     tRNA            678..750
                     /locus_tag="VHH53_05160"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:711..713,aa:Lys,seq:ctt)
     gene            928..2091
                     /locus_tag="VHH53_05165"
     CDS             928..2091
                     /locus_tag="VHH53_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX2289580.1"
                     /translation="MEQRYQAVMCVLADGLLVTEVAEKFGVSRQTVHSWLRRYEAGGL
                     DGLADRSHRPVSCPHRMPAEVEARVCELRRERPYWGPVRIGHQLGREGVDPVPSHMGI
                     WRALVRHGLIEPGARRKRLRTFKRWERARPMELWQLDVVGGVLLADGTECKVLTGIDD
                     HSRFCVAAGIMTRAVARAVCGIFAQSLRTHGVPDEVLTDNGKVFTGRFAARPVEVLFD
                     RICRENSITHRLTAPRSPTTTGKIERFHRSLRVEFLTGRVFDDLSSAQAELDSWVTDY
                     NTNRPHRGIGMATPAERFLVARTRPAPELDPHLIALHDARDPDEWITRKVSVNGVITV
                     AWQQISVGRHRERRRVDIHVLAEILEVWDGNELIKTVKRTSNGVVRKKKAQSD"
BASE COUNT          381 a          870 c          809 g          416 t
ORIGIN      
        1 cgatgcccgt ccgcagatgg cacacccgga tgcccgcctc cgtggccggt gcggtggcag
       61 cctcccaggc ggtgcagagt tcggccagga agccgccgcc cggccggctg gtctcggtga
      121 gcacctcgtc gccccggttc ccgtagaacc cgacccccga gccggagagc aacaccctgg
      181 gcggccgggc cagcttggcc agcgtctcgg ccagcagcgc ggtgccgcgc acccggctgt
      241 tccggatctt cgccttctgc tcctcgttcc accgcttgtt gccgattccc tcccccgcca
      301 ggtgtacgac cgcgtggact ccttcgagcc cgccggcgtc gatgtcgccg ccctcgggat
      361 cccagtggag ggcgccggcg ttgcccttcg accggctcag ggggatgacc cggtgcccgg
      421 cggtctccag cgccgctttc agcgcggtcc cgatcagtcc actcgacccg ctgacgacga
      481 cgtccatgac gattcccctg atgcggaggc ggcggcggcc gcgaatccat tggctcgcgc
      541 acccgacctt cgaccactgc cgccaaaaga gccatacgga ccgatctccc ccggatgctg
      601 tcacacccgg tgggctggtc gggacccggg gtccgcaacc tcccgggcgg accctgtact
      661 ctggcgtggc cgtaccagcg cccctagctc aactggcaga gcagcggact cttaatccgc
      721 gggttcaggg ttcgagtccc tgggggcgta cttccccaaa tgtttgagca cgcctgatgc
      781 ttgtcggatg ggtaacggct gacgttttcc gcggtatcgg ctgatgcttg acgcggtaac
      841 ggctgatgct tgacacctcg gaccccttgc tcctcggctc ttggccgggt cgaggaggtg
      901 ggccgggtgc tcaaggagct cagcaagatg gagcagcgtt atcaggccgt gatgtgtgtg
      961 ctggccgatg gtctgctggt gacggaggtg gccgagaagt tcggggtgtc tcgtcagacg
     1021 gtgcactcct ggttgcggcg ctatgaggcc ggtggtctcg acggcctggc tgatcggtcg
     1081 catcggccgg tgtcgtgtcc ccatcggatg ccggccgagg tggaggcccg ggtctgtgaa
     1141 ctgcggcggg agcggcccta ttggggtccg gtccggatcg gccatcagct tggtcgggag
     1201 ggtgtggatc cggtgccgtc acacatgggg atctggcggg ccctggtccg ccatggcctg
     1261 atcgagcctg gcgcccggcg caagcggctc cggaccttca agcggtggga acgagcccgg
     1321 cccatggaac tgtggcagct cgacgtcgtg ggcggggtgt tgttggccga cggaaccgag
     1381 tgcaaggttc tcaccggcat tgatgaccac tcacggttct gtgtcgctgc cgggatcatg
     1441 acccgagccg tggcccgggc ggtgtgcgga atcttcgccc agtcgctgcg gacccacggc
     1501 gtccccgacg aggtcctgac cgataacggc aaggtgttca ccggccgctt cgccgcccgg
     1561 ccggtcgagg tgctcttcga ccggatctgc cgggaaaaca gcatcaccca ccggctcacc
     1621 gccccccgct cacccaccac caccggcaag atcgagcgct tccaccgcag tctgcgggtc
     1681 gagtttctga ccggccgggt cttcgacgac ctgagctccg cccaggccga actcgacagc
     1741 tgggtcaccg actacaacac caaccgaccc caccgcggca tcggcatggc cacgccggcc
     1801 gaacggttcc tcgtcgcccg gacccggccg gcgcccgaac tcgaccccca cctcatcgcc
     1861 ctccacgacg cccgcgaccc cgacgagtgg atcacgcgta aggtctccgt caacggcgtc
     1921 atcaccgtgg cctggcagca gatctccgtc ggccgccacc gcgaacgacg ccgggtcgac
     1981 atccacgtcc tggccgagat cctcgaggtc tgggacggca acgaactgat caagaccgtc
     2041 aaacgaacat ccaacggcgt agtccgcaaa aagaaagccc agtccgactg accaaccaac
     2101 cccgaggtgt caaccatcac ccgttactga tctgtccatc atcaggcgtg atccctcagg
     2161 cgtaccaccc aaagcgcagg tcacgctgag tacctgttct ttgatgaccc tgcgccgcag
     2221 gtttaccgac gccccgcatc cccgtggtcg ccaccacagg cgctgttccg agagcagcgc
     2281 gggcgaggcc ggtcatcgac tcgtacaact tctcatgacc gcctggccgg gggatcggcg
     2341 cgcagctcca aaggcgacgg tgggagcatc ctccggttga ccagcgatct tggcgacgcg
     2401 gatgagtgcc gcgctggtgc cggaaggccg ggcgccgcct cggggtgacg gttcggaccg
     2461 gtatctcggg tgaccg
//
LOCUS       DATADN010000393         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1108557, whole genome shotgun sequence.
ACCESSION   DATADN010000393 DATADN010000000
VERSION     DATADN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108557"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..276
                     /locus_tag="VHH53_05170"
     tRNA            192..276
                     /locus_tag="VHH53_05170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Leu,seq:tag)
     gene            529..2034
                     /locus_tag="VHH53_05175"
     CDS             529..2034
                     /locus_tag="VHH53_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289581.1"
                     /translation="MSTSARAQQVAPLVQPGDRFPDGETPETFQGPVGEAKCGPGSNP
                     ETSDIQGSVPVPDRESGRSSQGYWCNLELVGRYGPDDPQPFEGAGWQLGRYGDCAYYS
                     QRMVNPMRVDARQRPGTVVVDVSDPTDPKFSDNIATLGMLDPWENLKVSRQRGLLIGA
                     SVIDGLGVGYMGIYDVTGDCAHPKKLFDGRAAWVTHEGNLASDGMTYYAGGMQPGIVS
                     AVDISDPTNPRLLTTFFAKHSIHGYSTSPDGKRLFISHVNDDWQYTLNVGGSGWRAGE
                     PGEESLLRGNGIGIYDVSEIQERKPDPKVRLLAALEYQDGQMGQHSLNFVKDGVPYVV
                     HVSEAGHGGARIIDISDERNPKVISKLKTEIMMPDHHDRAVAETKRLGKESGGDLPFG
                     YNIHYCNLDRGIEPTMLACSAFESGLRLFDIRDLRAPKELGYYNPGGDGTRMPGGWCG
                     VYSGYAPAMPQFEPTLKHIWFTDQCRGFYVVKPTNGTWISDVTEASLSHGN"
     gene            2036..>2260
                     /locus_tag="VHH53_05180"
     CDS             2036..>2260
                     /locus_tag="VHH53_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289582.1"
                     /translation="MVPCPIPAALDGFARAVGRRHVVTGRQSLSAAGRTTYATHQRLA
                     AIVRPGTVAQVQAVMRVAHEHGVAVHPVSGG"
BASE COUNT          405 a          770 c          753 g          332 t
ORIGIN      
        1 gccagcgccg ggttggcagc ctgcgccacg agtcttctcc ccctccttcg cccggcggat
       61 ttccgaccag tggccggtga cgctaccgtg cccgctctcc cggcaggtgt ggccgcctga
      121 atggccgccc caccgtccgg aaaaaaatca ctcatccacc ggccaacctg cgccgtagta
      181 acgatcagca cgcgagcgtg gcgaaatcag gcagacgcgc cgggtttagg tcccggtccc
      241 cgcaaggggg tgagggttca aatccctccg ctcgcacagg gaatggccga gcgacacgac
      301 gccggacgga atccgcccgc agtgcaggcg gttgcggttc agtaattgaa ctagtcccgg
      361 tcggctgcca tatggcaggt tgccgccgtg gggagttacg gccattcctg ccgccgtcga
      421 ggcgcagcgg tgacaagggc cgcgaggttg cgaccgacga tcgtcgtcgc catggccttg
      481 attggaattg catggccgct ggcgcccggg gcagccaacc tgccctcgat gagcaccagc
      541 gcccgcgccc agcaagtggc gccgctggtc cagccgggtg accgcttccc ggacggggag
      601 acaccagaga cgttccaggg tcccgtcggg gaggcgaagt gcggcccggg ttcgaacccc
      661 gagaccagcg acatccaggg gtcggtgccg gtccccgacc gggagagcgg ccggtcatcg
      721 cagggttatt ggtgcaatct cgagctggtt ggtcgctacg gtcccgacga cccccaaccc
      781 ttcgaagggg cagggtggca actgggccgc tacggcgact gcgcctatta cagccagcgg
      841 atggtgaatc ccatgagggt ggacgcccgc cagcggccgg gcacggtggt ggtcgatgtg
      901 tccgacccga ccgatccgaa gttctccgac aacatcgcca ccctggggat gctcgacccc
      961 tgggagaacc tcaaggtgtc ccggcagcgc ggcctcctga tcggggccag cgtgatcgac
     1021 ggcctcggcg tcggttacat ggggatctac gacgtgacgg gcgactgcgc ccaccccaag
     1081 aagctcttcg acggccgggc ggcgtgggtc acccacgaag gcaacctagc cagcgacggg
     1141 atgacctact acgccggagg gatgcagccg ggaatcgtct ccgccgtgga catctccgat
     1201 ccgaccaacc ccaggctgct gaccacgttc ttcgccaagc acagcatcca cggctacagc
     1261 acgagccccg acgggaagcg cctgttcatc tcccacgtca atgacgattg gcagtacacg
     1321 ctgaacgtcg ggggcagcgg ctggcgggcc ggggagccag gcgaggagag cctgctccgc
     1381 ggcaacggca tcgggatcta cgacgtgagc gagatccagg agcgcaagcc cgatcccaag
     1441 gtgcgcctcc tcgccgccct ggagtaccag gacggtcaga tgggccagca cagcctgaat
     1501 ttcgtcaagg acggcgttcc ctacgtcgtc cacgtgagcg aggccggcca cggaggggcg
     1561 cgcatcatcg acatcagcga cgagcgcaac ccgaaggtga tcagcaagct gaagaccgag
     1621 atcatgatgc ccgaccacca cgatcgggcc gtggccgaga ccaaacggtt gggaaaggag
     1681 tctggcggcg acctcccctt cggatacaac atccactact gcaatctcga ccggggaatc
     1741 gagcccacga tgctggcctg cagcgccttc gagtccggcc tgcgactgtt cgatatccgg
     1801 gacctcaggg ccccgaagga actcggctac tacaaccccg gcggggatgg cacccgcatg
     1861 cccggaggct ggtgcggggt ctactccggc tacgcgccgg cgatgcccca gttcgagccg
     1921 accctcaagc acatctggtt caccgaccag tgccggggct tctacgtggt caagccgacc
     1981 aacggcacct ggatcagcga cgtgaccgag gcatctctca gtcacggcaa ctgaattggt
     2041 gccctgtccc atacccgcag cactggacgg gttcgcgcgg gccgtcggcc ggcgccacgt
     2101 ggtcacgggc cgtcagtctc tgagtgccgc cggtcgcacc acctacgcca cgcaccaacg
     2161 gctggcggcg atcgtccggc cggggaccgt ggcgcaggtt caggcggtga tgcgggtcgc
     2221 ccacgagcac ggtgtcgccg tgcacccggt cagcggcggc
//
LOCUS       DATADN010000394         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1409010, whole genome shotgun sequence.
ACCESSION   DATADN010000394 DATADN010000000
VERSION     DATADN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409010"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..2069)
                     /locus_tag="VHH53_05185"
     CDS             complement(327..2069)
                     /locus_tag="VHH53_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX2289583.1"
                     /translation="MFLRETRRTNRDGSVVSYLQLAHNQRHPVSGNPVAKVIHNFGRA
                     DRVDRAALARLVSSISRFLSPEQAVTAAGAAEFAGEVEVLDSRRLGAAWTLDRIWERL
                     GIGAAIRRVATGRRLAGEAVERVVFALVAQRACEPGSKLAATGWVAQRVAIADCAGFT
                     DDAAYAAMDFLLDALGEIAAEVFGSVAHLLNLDLDIVFVDTTSTYWETETTDELAELA
                     DEPGDDETSNPVEAGTRAFGHSKDHRGDLPQVVIAMAVTRDGVPVRCWTFPGDTADTA
                     IIRTVKDDLDGWGLRRLVWVADRGFACAANRAYLTRGGGHYIHAEKLRQANTEAAAAL
                     ARAGRYRTVADNLRVKEIHVAPGGDGDGDGGARTQRFVICHNPEQAERDQRVRANLVA
                     HLQQLIDGSDSWTPRRRDELVGSLKTKPGLRRYLRRTKTGLLRVDHGAIRREAHLDGK
                     WLLRTSDLTLTPDDLAAAYKQLLAVERGWRDMKGALGLRPIFHYREDRIRAHIQLCWL
                     ALLLLRVIENATGDTWRNIRHELDRMHLVTLATNAGQIAQRTTSTAGQQTLLRALDLP
                     EPPRIFDYTLPSST"
BASE COUNT          333 a          846 c          844 g          363 t
ORIGIN      
        1 ccggtgtgcc ctcgggcaac cgccatccgc cggcgtccgg accgagccgg atgaggacct
       61 cggtggtgga acacagcccc gccagctggt cgcgccgtac cccggtcagc acgagtgcct
      121 cctcgtccca gtcccgcgcc gccagcccgt acggccccgt cgccgacgcg accgactcgt
      181 cgaccgccag cagccgggtc agccggtgac cggacttccg aagatcacga gcacacgagg
      241 gcctgaatcc gtggttgacc tgcgggaatg ccgaagaggc cggccggggt cgtgttacta
      301 cacgggttgg ccggtggggt cggggttcag gtgctgctgg ggagggtgta gtcgaagatc
      361 cttgggggtt cgggcaggtc gagcgcgcgg agcagggtct gttggccggc ggtgctcgtg
      421 gtgcgttggg cgatctgccc ggcgttggtg gccagggtga ccaggtgcat ccggtcgagt
      481 tcgtggcgga tgttgcgcca ggtgtcgccg gtggcgttct cgatcacccg caggagcagc
      541 agcgccagcc agcacagctg gatgtgggcg cggatgcggt cctcgcggta gtggaagatc
      601 gggcgcagcc ccagcgcgcc cttcatgtcg cgccacccgc gttccaccgc gagcagttgc
      661 ttgtaggccg cggccaggtc gtcgggggtg agggtgaggt cggaggtgcg cagcagccac
      721 ttgccgtcca ggtgcgcctc gcggcggatc gcgccgtggt ccacgcgcag cagcccggtc
      781 ttggtgcggc gcaggtagcg gcgcagcccc ggcttggtct tgagcgagcc gacgagttcg
      841 tcacggcggc gcggggtcca gctgtccgag ccgtcgatca gctgctgcag gtgcgcgacc
      901 aggttggccc ggacccgctg gtcgcgctcg gcctgctcgg ggttgtggca gatcacgaac
      961 cgctgggtgc gcgccccgcc atcgccgtcg ccgtccccgc ccggggccac gtggatctcc
     1021 ttgacccgca ggttgtcggc caccgtgcgg tagcgaccgg ccctcgcgag cgcggcggcg
     1081 gcctcggtgt tggcctggcg cagcttctcg gcgtggatgt agtgcccgcc accgcgggtc
     1141 aggtaggccc ggttggccgc gcaggcgaac ccgcggtcgg ccacccagac cagccggcgc
     1201 agcccccacc cgtccaggtc gtccttgacg gtgcggatga tcgcggtgtc ggcggtgtcc
     1261 ccggggaagg tccagcaccg caccggcacc ccgtcccggg tgaccgccat cgcgatcacc
     1321 acctgcggca gatcgccgcg atggtccttg gagtgcccaa acgcgcgggt gccggcctcg
     1381 accgggttgc tggtctcgtc gtcgccgggc tcgtcggcca gctcggccag ttcatcggtg
     1441 gtctcggtct cccagtacgt gctcgtggtg tccacgaaga cgatgtccag atcaagattg
     1501 agcaggtggg cgaccgagcc gaacacctcc gcggcgatct cgcccagcgc gtcgagcagg
     1561 aagtccatcg ccgcgtacgc ggcgtcgtcg gtgaaccccg cacagtcggc gatcgccacc
     1621 cgctgcgcca cccagccggt cgcggccagc ttcgacccgg gctcgcacgc ccgctgcgcg
     1681 accagcgcga acaccacccg ctcgaccgcc tcgccggcca ggcgtcgccc ggtggcgacc
     1741 cggcggatcg cggcaccgat cccgaggcgc tcccagatcc gatccagggt ccacgccgcg
     1801 cccagccggc gcgagtccag cacctccacc tcgccggcga actcggcggc accggccgcg
     1861 gtgaccgcct gctccgggga caggaaccgc gagatcgacg acaccagccg ggccagcgcc
     1921 gcccggtcca cccgatcggc ccgcccgaag ttgtggatca ccttggcgac cgggttcccg
     1981 ctgaccgggt gccgctggtt gtgcgccaac tgcagatacg acaccaccga gccgtcccgg
     2041 ttggtccgcc gcgtctcccg caagaacacg cctacaagat agcggcatca ctacagctca
     2101 agccgcgcga cacgcaccga gtcgtgtgcc tacagcgttt ccggctctgg aacgtccggc
     2161 agcatacctg atcaggccag acgtgatcga tcacgcccac atgtgcctat gatcttcgga
     2221 agtccggtct gaccgcgcga ccgggcagcc gttcacgccg ccgcgcgggt tgtgagctac
     2281 gcccacgcct tgtccgcgcg gtaaaccctg acaattcatg gcgatcttgc tggggtgagt
     2341 gtcggaacgg agaaaggtgc tcctgacctg ggagaatgag ggtgtc
//
LOCUS       DATADN010000395         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_356315, whole genome shotgun sequence.
ACCESSION   DATADN010000395 DATADN010000000
VERSION     DATADN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356315"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1304)
                     /locus_tag="VHH53_05190"
     CDS             complement(90..1304)
                     /locus_tag="VHH53_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2289584.1"
                     /translation="MSDGPEGASSCGHYRMRRIGKALVTAVACLVFTVTAAGWTTTQW
                     LDGQLRNVLALDPDSAAITDADAQRGDENVLLVGSDSRMGARQSDGVGSTDMVGGARS
                     DTVMIAHLPADRSRAVVVSFPRDLQIQRPPCEMWDAASGSYTGRIDAGASVAKLNTAY
                     EIGGPLCVTKVVQDLSGLAVTRFVGVDFHGFKHMVDAVGGVRICVERPLKDSQLGTII
                     PQPGYSTISGSTALNYVRARHVTGDPTSDYGRITRQQHFLSALLGQVLSPDVLLNPAK
                     LRALITAVAANTVGQNIDSSALLTVAQSVQGLDPANVAFVTVPTTGLANEHGNEELRS
                     EDNAALFRTIIDDTALPGPPPPEAPAEARFTAPAAPQPPTPPGAPQPPAAGQSPAAAP
                     LATVHSADSVCS"
     gene            complement(1373..2512)
                     /gene="dusB"
                     /locus_tag="VHH53_05195"
     CDS             complement(1373..2512)
                     /gene="dusB"
                     /locus_tag="VHH53_05195"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX2289585.1"
                     /translation="MAAVRPVLRIGPYRVDPPVVLAPMAGITNVAFRRLCREHGAGLY
                     VCEMITCRALVERHPSTLRMVSFDEAEQPRSMQLYGVDPVTVRKAVEMVRDEDLADHV
                     DLNFGCPVPKVTRRGGGAALPYKRALFGQIVRSAVEAAGHLPVTVKFRIGIDDEHRTY
                     LDAGRIAQDAGVAAVALHGRTAAQRYSGSADWDAIGALKQAVTSIPVLGNGDIFAAAD
                     AIRMVEHTGCDGVVVGRGCLGRPWLFAELEAAFREQHRPRSIPAPPNLGQVAAILRRH
                     AGLLAEHLGADKGLRDLRKHISWYLHGFPVGGDVRRALGMVSSLAELDGLLAQLDPDA
                     AFPPGADGPRGRQGSPGRVVLPEGWLDDPADLTVPADAELAHSGG"
BASE COUNT          387 a          882 c          882 g          400 t
ORIGIN      
        1 gtccagctcg gcgtggaaca tctctcgcat tggcccgagg gtacggccga cgatcgccgc
       61 cgtctcgatg accacggcgc tcgcgcgcct cagctgcaca cggaatcggc actgtggacg
      121 gtggccagtg gggcggcggc cggggactgg ccagcggccg ggggctgcgg tgcgcccgga
      181 ggtgttggtg gttgcggcgc tgccggtgcg gtgaaacggg cctcggcagg ggcttcaggt
      241 ggtggcgggc ctggcagcgc ggtgtcgtcg atgatcgtcc ggaacagcgc cgcgttatcc
      301 tccgaccgga gttcctcgtt gccgtgctcg ttggccaatc cagtggtggg caccgtgacg
      361 aaggcgacgt tggcggggtc cagcccctgc accgactggg ccaccgtcag cagcgccgac
      421 gagtcgatgt tctggccgac ggtattcgcg gcgaccgcgg tgatcagcgc gcgtaacttt
      481 gccgggttga gcagtacgtc cggagacagc acctggccca gcagcgcgga caggaagtgc
      541 tgttgacggg tgatccggcc gtagtccgat gtcgggtcac cggtgacatg ccgggcccgg
      601 acatagttca gggcggtgct gcccgagatc gtgctatagc ccggctgcgg gatgatcgta
      661 ccgagctggc tgtccttgag cgggcgctcc acgcagatcc gtaccccacc cacagcgtcg
      721 accatgtgtt tgaatccgtg gaagtcgact ccgacgaacc gggtcacggc cagcccggac
      781 aggtcttgga cgactttggt gacgcacaat ggcccaccga tctcgtaggc ggtgttcaac
      841 ttggcgacgc tggcgccggc gtcgatccgg ccggtgtagg aacccgaagc cgcgtcccac
      901 atttcacacg gcggtcgctg gatctgcagg tctcgcggga aggacaccac caccgcgcgg
      961 gaacggtcgg caggcagatg agcgatcatc acggtgtccg agcgagcgcc tccgaccatg
     1021 tcggtgctgc cgacgccgtc gctctggcgg gcacccatcc ggctgtccga gccgaccagc
     1081 aggacgttct cgtcgcctcg ttgggcgtct gcgtccgtga tcgccgcgga gtccgggtcg
     1141 agcgcgagca cattgcgcag ctgtccgtcc agccactgcg tggtggtcca gcctgcggca
     1201 gtgaccgtga agaccaggca ggccaccgca gtcaccaacg ccttaccgat ccgccgcatg
     1261 cggtagtgcc cgcaggacga ggcaccctcc ggcccgtcgg acaccccgac ctcctcccct
     1321 ggaccaggcg cgttaggccg tccgggggat gcatcgtact acgtgctcta cctcagcccc
     1381 cggaatgggc taattcggcg tccgcgggaa cggtgaggtc cgccggatcg tccagccagc
     1441 cctcgggaag cacgacccgt ccgggcgacc cctggcggcc tcggggaccg tcggcgccgg
     1501 gcgggaatgc ggcatcggga tccagctgcg cgagtagacc atccagctcg gcgaggctgg
     1561 acaccattcc cagcgcgcgc cgtacgtcgc ccccgaccgg aaaaccgtgc agataccagg
     1621 agatgtgctt gcgcaggtca cgcaggccct tgtcggcacc gaggtgctcg gcgagtaacc
     1681 cggcgtggcg acgcaggatt gccgcgacct gcccgaggtt cgggggagcc gggatgctgc
     1741 gtggtctgtg ttgttctcgg aaggcggcct ccagctcggc gaacagccac ggtcgcccca
     1801 ggcagccgcg gcctaccacg accccgtcgc agccggtgtg ctcgaccatc cggatggcgt
     1861 ccgcggcggc gaagatgtcc ccgttgccca gcaccggaat cgacgtgacg gcctgcttga
     1921 gcgcgccgat ggcgtcccag tcggcactgc cggagtagcg ctgcgcggcg gtgcgcccgt
     1981 gcagggctac cgcggctact cccgcgtcct gcgcgatgcg accggcgtcg aggtacgtgc
     2041 ggtgttcgtc gtcgatgccg atccggaact tcaccgtgac tgggaggtgc ccggccgctt
     2101 cgaccgccga ccggacgatc tggccgaaca gggcccgctt gtagggcagc gcggcaccgc
     2161 cccctcggcg ggtgaccttc ggcaccgggc aaccgaagtt gaggtcgaca tgatcagcaa
     2221 gatcctcgtc ccgcaccatc tctacggcct tgcgcacggt caccggatcg accccgtaca
     2281 gctgcatcga gcggggttgc tcggcttcat cgaagctgac catccgcagg gtgctggggt
     2341 gccgttccac cagtgcgcga caggtgatca tctcgcagac gtacagcccc gctccgtgct
     2401 cccggcacag ccgacggaag gccacgttcg tgatcccggc catcggagcg agcacgaccg
     2461 gcgggtcgac ccggtaagga ccgatccgca gcactggtcg caccgcagcc acccgcccat
     2521 catccctccc cccacccccc cgcgtgtccg c
//
LOCUS       DATADN010000396         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_32640, whole genome shotgun sequence.
ACCESSION   DATADN010000396 DATADN010000000
VERSION     DATADN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32640"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="VHH53_05200"
     CDS             <1..1346
                     /locus_tag="VHH53_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2289586.1"
                     /translation="LEHQAYHDPLTKLANRAMVTDRLGRIFAEPAGRRRVGLCFLDLD
                     GFKVVNDSLGHEIGDQLLIAVASRLIRCCDSEQLVARMGGDEFVIIVEDTAGEHDVAA
                     LADEVLAVMAAPIRVGDHELTVTTSIGVVERPVADTNPADLMQAADITLYRAKAEGKA
                     RYAMFDQHRNDSEVARFTLSATLLAAIDRGEFFIDYQPLIALKDGTLRGAEALVRWRH
                     PTLGVLGPERFISLAEETGSIVQLGHWVLTQACQQARAWHDAFGETAPLVSVNLAPRQ
                     LNEPALVAEVATILTDNDLDPSCLQLELTEQSLMGDEAAPLKALTKLYHMGVRIAIDD
                     FGTGYSNFSYLRRLPMHELKLASSFAVSLRSPDADLVDEQIVATLVSLAHALDLTVTA
                     EGVEANVQLERFRALGCDAGQGSLFGLPGTAEQIDTRLRAAMSPCDARKKRRSPS"
     gene            1404..1589
                     /locus_tag="VHH53_05205"
     CDS             1404..1589
                     /locus_tag="VHH53_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289587.1"
                     /translation="MLSGVRELAVGTALAIGLLIPLTAAFVVGTMVAAGVTAHRRNGF
                     DSRKAMSTSSWSALSAQ"
BASE COUNT          269 a          504 c          534 g          299 t
ORIGIN      
        1 ggttggaaca ccaggcttac cacgatccac tgaccaagct ggctaatcgg gcgatggtca
       61 ccgaccggct gggacggatc ttcgccgaac cggcgggccg gcgccgggtc gggttgtgct
      121 ttttggacct ggacgggttc aaagtggtca acgacagcct gggccacgag atcggcgacc
      181 agttgctgat tgccgtcgcg tcgcggttga tccgctgctg cgattcggag cagctggtgg
      241 ctcggatggg tggcgacgag ttcgtgatca tcgtcgaaga caccgccgga gagcacgacg
      301 tggccgcact ggccgatgag gtgctggctg tcatggccgc tccgatccga gtcggcgatc
      361 acgagctgac cgtcacgacg agcattggtg tcgtcgagcg gccggtggct gacaccaatc
      421 ccgcggacct catgcaggcc gccgacatca cgctgtaccg ggcgaaagcc gagggtaagg
      481 cgcgctatgc gatgttcgat cagcaccgca acgacagtga ggtggcccgt ttcacactgt
      541 ccgccacgct gcttgccgcg atcgaccgcg gtgagttctt catcgactac cagccgttga
      601 tcgcgctgaa ggacggcacc ctgcgcgggg ccgaggctct ggtgcgctgg cgccatccga
      661 cactaggtgt gctcggaccc gaacggttca tcagcctggc cgaggagacc ggttcgatcg
      721 tgcaactcgg gcactgggtg ctcacccaag cctgccagca ggcccgtgcc tggcacgacg
      781 cgttcggtga gaccgcgccg ctggtcagcg ttaacctcgc accccggcag ttgaacgagc
      841 ctgccctggt ggctgaggtt gcgacgatcc tgactgacaa cgatctcgac ccgtcctgcc
      901 tgcaactcga actcaccgag cagtctttga tgggcgacga ggcagctccg ctcaaagcgc
      961 tgacaaagct ctaccacatg ggcgtgcgaa tcgccatcga cgacttcggc accggctact
     1021 ccaacttctc ctacctgcgt cggctgccga tgcatgagct caagctggcc agttcgttcg
     1081 ccgtgagcct gcgatctccc gacgccgacc tcgtggacga gcagatcgtg gccaccttgg
     1141 tgagcctggc acatgccttg gacctgaccg tgaccgccga gggtgtggag gccaatgtgc
     1201 agctcgagcg attccgggcg ttggggtgtg acgcaggtca ggggtcactg ttcggcttac
     1261 ctggcactgc tgagcaaatc gatacccggc tgcgggcggc catgtcaccg tgcgatgccc
     1321 gcaagaagag gcgatcaccg agctagacac cgtcgacatc gcggcagcgt tgctgcgctt
     1381 tgcggccgcc gaagctgcac gcggtgctgt ccggtgtcag agagctcgcc gttgggaccg
     1441 ctctggcgat cgggctgctc atcccgctga cggcggcctt cgttgtcggc accatggtgg
     1501 ctgccggggt tacagcgcac cgccgcaacg gatttgattc gaggaaggct atgagtacgt
     1561 cctcatggtc ggcgttgtct gcgcagtgat cgggttactc ggtccg
//
LOCUS       DATADN010000397         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_959294, whole genome shotgun sequence.
ACCESSION   DATADN010000397 DATADN010000000
VERSION     DATADN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959294"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /gene="mycP"
                     /locus_tag="VHH53_05210"
     CDS             complement(<1..1171)
                     /gene="mycP"
                     /locus_tag="VHH53_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HEX2289588.1"
                     /translation="MRAVAGRGRSLLVTSLAALLCMTGPGLSVAAEPEIRPPGTPPAV
                     NPAALPTPASVSALPYIVNNRATRQTGPVQQQADCQRQATANVEAEPAGQRRLRIRQA
                     QQFATGQGQRVAVIDSGVQPHPRLAGRLIDGGDYIENRSGLFDCDGHGTAVAGLVAAA
                     PDPASRFVGVAPDAEILSIRQASTSYSVPLRQLDTGQEIRSRVTGDTVSMAQAVMRAV
                     QLGATVINISEAACFPAERDQVNAPDLQAAVHHAVERNVVVVVAAANASDTCKQNSPG
                     VVTTISSPGWFDDDVLTVAATDDNGDAADFSIRGPWVDVAAPGTGAVSLSSAGPDLIT
                     ELIDADGQARPLDGTSFATPYVAGLVALVRDRFSHLSARQVMRRIEQTAQHTAGPG"
     gene            complement(1168..2499)
                     /gene="eccD"
                     /locus_tag="VHH53_05215"
     CDS             complement(1168..2499)
                     /gene="eccD"
                     /locus_tag="VHH53_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HEX2289589.1"
                     /translation="MTVVAPRTRIDLALPSDVAVANLLPMVLEMAGEAASDGGSRHGG
                     WCLAKLSGDAIDPDRSLSSLGVVDGDLLQLRHRCDSPPRPLFDDVVEAIAVSAPDSYR
                     PWTQHSARTLGLVAAGLALLAGAVALFRAGPGLGVAAIAGAGAVVVLVAGAVVARVYG
                     ETATGVVVAAGSVPLAFVSGLYVVPGSVQAENLLLACTLALVLAIASIMLLGDGITAF
                     VASATATALGALAFLIASLVDRPDAGIAAAAAAVALGGISAVPRLTIQLSRLPIPQIP
                     GGAEDLQRDSGFPDYVAIERRAGLAHEYMTGMIIGSGAVAAAGAVVASTGGALGIVLA
                     VVVAAVLLLRARSYANGSQATALLASGMVAVAGLLAGLLITASQALLLALFACLLMLA
                     ALALALGVVLPSRRFSPVLRRSVDVIEGMLIAAVLPLALGVLDLYNTFRAL"
BASE COUNT          451 a          927 c          902 g          386 t
ORIGIN      
        1 caccggggcc cgcggtgtgc tgtgcggtct gctcaatgcg acgcatcacc tgacgcgcgg
       61 acagatggct gaaccgatcg cggaccagcg caacgagtcc ggcgacatac ggcgtggcga
      121 agctggttcc gtcgagcggg cgcgcttggc cgtcggcgtc aatgagctcg gtgatcagat
      181 ccggtccggc cgaagacagg gacaccgcgc cggtgcctgg tgcggcgaca tcaacccacg
      241 gtccgcggat cgagaaatcc gctgcatcgc cattgtcgtc agtcgcggcg acggtgagca
      301 cgtcgtcgtc gaaccagcct ggtgacgaga tcgtggtgac cacaccaggg ctgttctgct
      361 tgcaggtatc actggcattg gcggcggcca ccaccacgac gacattgcgt tccaccgcgt
      421 ggtgcacggc ggcctgcagg tccggggcgt tgacctgatc gcgctcggcc gggaaacacg
      481 cagcttccga gatgttgatc accgttgcgc caagctgcac cgcacgcatc acggcctggg
      541 ccatgctcac ggtatccccg gtgacccggc ttcgaatctc ctggccggtg tcgagctggc
      601 gtagcggtac cgaataagag gtgctggcct ggcggatgga caggatctcc gcatccgggg
      661 ccaccccgac gaaccgacta gccggatcag gcgccgcggc aacgaggccg gccaccgcgg
      721 tgccgtggcc gtcgcagtcg aacaggccac tgcggttctc gatgtagtcc ccgccgtcga
      781 tcaacctgcc ggccagcctt ggatgtggct gtacgccgct gtcgatgacg gccacccgct
      841 ggccttgtcc ggtcgcgaac tgctgtgcct gccggattcg tagccgccgt tggccggccg
      901 gctcggcctc gacgttggcg gttgcctgcc gctggcagtc agcttgctgc tgcaccgggc
      961 cggtctgccg ggtagcccgg ttgttcacga tgtagggcag cgcgctgacg ctggccgggg
     1021 tcggcagggc cgccggattc accgcaggtg gcgtgcctgg cggccggatc tccggttcgg
     1081 cggcaaccga caaccccgga cccgtcatgc acagcaacgc ggccaggctg gtgacgagca
     1141 gtgacctacc acgaccggcg actgctctca cagcgccctg aaggtgttgt agaggtccag
     1201 cacacccagt gccagcggca gcaccgcggc aatgagcatc ccttcgatca cgtccactga
     1261 gcgccgtagc accggggaga accggcggct cggcagcacc actcccagtg ccagagcgag
     1321 cgctgcgagc atcaggaggc aggcgaagag cgccagcaac agggcttggc tggccgtgat
     1381 gagcaacccg gccagcagcc cggctactgc taccatccca ctggccagca gcgcggttgc
     1441 ctggctaccg ttggcgtagg agcgcgcccg caagagcagg acggcggcga caaccacggc
     1501 aagcacgatc cccaacgcac ctccggtcga ggcgacgacc gcacccgctg ccgccaccgc
     1561 accggatcca atgatcatgc cggtcatgta ctcgtgtgcc aggcccgccc ggcgctcgat
     1621 cgcgacgtag tccggaaacc cggagtcacg ctgcaggtcc tcagcgccac ccggaatctg
     1681 gggtatcggg agccgggaca gttggatggt cagccgcggc actgccgaga taccgcccag
     1741 cgcgaccgct gcggccgccg ccgcgatgcc cgcgtcagga cgatcgacca ggctggcgat
     1801 caggaaggcc agcgcgccga gcgcagtggc ggtggcactg gccacgaacg ccgtgatgcc
     1861 gtcgccgagc agcatgatcg aggcgatggc gagcaccagg gccagcgtgc aggccagcaa
     1921 gagattctcc gcttgcacgc tgccgggcac gacatagagc ccgctgacga aagccagcgg
     1981 cacgctgcct gccgccacca ctacgccagt agcggtttcg ccgtacaccc gggcgactac
     2041 tgcacccgcg accagcacga ctaccgcgcc tgcgccagcg atggcggcga cgccgaggcc
     2101 cggtccggcg cgaaacagcg caacagcacc ggcgagcaac gccaaccccg ccgcgaccag
     2161 cccgagggtg cgggcgctgt gctgggtcca tggacggtaa ctgtccggtg cggataccgc
     2221 gatggcttcc acgacgtcgt cgaatagtgg gcgcggaggg ctgtcgcagc gatgccggag
     2281 ttgcagtaga tcgccgtcga ctactcccaa cgagctcagc gaacggtccg gatcgatcgc
     2341 gtcaccgctg agtttggcca ggcaccagcc gccgtgcctg ctgcccccgt cggaagcggc
     2401 ttcgccggcc atctcaagca ccatgggaag caggttcgcc accgcgacgt ccgacggcag
     2461 cgccaggtcg atgcgtgtcc gcggtgccac cactgtcacc cggctgaaca cggtgggcgg
     2521 cgcaaccgtc agctcagcaa ccgacggccg caccgcggat actccattgg ttctggttgg
     2581 caaccgctgc accctctcgc tcggcctgcc gaccgttggt atgcacatcg gtgcgaggtc
     2641 acggcggcct tctcggtgcg gtggag
//
LOCUS       DATADN010000398         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1109541, whole genome shotgun sequence.
ACCESSION   DATADN010000398 DATADN010000000
VERSION     DATADN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109541"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHH53_05220"
     CDS             complement(<1..224)
                     /locus_tag="VHH53_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289590.1"
                     /translation="MKSADDIINLTSITQNPLRPTNLRQDLEGTAGSIALLQIYALQA
                     LQQSVLNPALFDAGLRGQLAGIAQHQAVVRR"
     gene            complement(882..>1715)
                     /locus_tag="VHH53_05225"
     CDS             complement(882..>1715)
                     /locus_tag="VHH53_05225"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2289591.1"
                     /translation="LDSAAAYLVREFQRMGLQGGAGNGEFIQRFAPRGRGPAVAPNVI
                     AIFPGADPALRNEYVVLSAHIDHVGVGRPVRGDSIYNGADDDASGTSALLEVAEAFAS
                     MERRPARSILFLGVSGEEQGLLGSHHFSENPTVPLQSIVANLNIDMIGRNEPGDIVVI
                     GKTYSSLGPLVERMAAEHRGMLGLTAADDRWPGERFFFRSDHFNFARKEIPAVFFFAG
                     PHEDYHEPSDEVERIDADKAARVTRLVFLTAHVLATDPQRPQWTQQGLAEVRRMTGGQ
                     R"
BASE COUNT          297 a          548 c          580 g          290 t
ORIGIN      
        1 cgacggacca cggcctggtg ctgcgcgatg ccggccagct ggccgcgcag gccggcgtcg
       61 aacagcgcgg ggttcagcac cgactgctgc agcgcctgga gcgcatagat ctggagcagc
      121 gcgatgcttc cggcggtgcc ctccagatcc tgccgcaggt tcgtgggccg cagggggttc
      181 tgggtgatgc tcgtgagatt gatgatgtcg tcggctgact tcatggtgac cttccgggaa
      241 agaggacaat gcgcatggat ggacactgcc ggggccgggg aacggctcct cctgccagcg
      301 ggccaggcga acgaatggcc agttacctcc tgatggggtg agcgggcacc gcccgcgggt
      361 ttcggaagac gaatatcttt ttcagtacaa aaatcacaca agtgtacgcg gcggtgcccc
      421 actctcatgc cacgtgtcca tcgttggccg acaccagttc ttttctgtga aaaatttgaa
      481 aaaatccatt ttgtccatcc tactctcaca gcgtggacgt agcaggaggt atcaggtagt
      541 aacgcatcaa atggaccagt aattacgctc tctgtgcacc ttctgtcgat gcgctggagt
      601 cgcagtcggt tactggtgac gcgcccgcga attcggcgaa aattcatcca cggagtatac
      661 gccacagacg tggcccaatg ggacgtgctg gtgcggcggg aaagggacag cagcggcaac
      721 taaagatctt tacacgttat tatttgtggg atcgaaaggt atgccgggtg ggcgtaaaaa
      781 ggacatcgcc ctctcccggg gatcccggga gagggcgatc agcgccgcgg cgtggccaga
      841 gagggaaagg gttccgcgac cctttccaac gggagcggaa cctaccgctg cccgccggtc
      901 atcctgcgca cctcggccag gccctgctgc gtccattgcg ggcgctgcgg gtcggtggcg
      961 aggacgtggg cggtgaggaa gaccagccgg gtcacgcggg ccgccttgtc ggcgtcgatc
     1021 cgctccacct cgtccgaggg ctcgtggtag tcctcgtgcg ggccggcgaa gaagaagacg
     1081 gccgggatct ccttgcgcgc gaagttgaag tggtcgctgc ggaagaagaa gcgctcgccc
     1141 ggccagcggt cgtcggccgc cgtaagcccc agcatccccc ggtgctccgc cgccatccgc
     1201 tccaccagcg ggcccagcga cgagtaggtc ttgccgatca ccacgatgtc gccgggctcg
     1261 ttgcggccga tcatgtcgat gttgaggttg gccacgatgg actgcagcgg cacggtgggg
     1321 ttctcgctga agtggtgcga gcccagcagc ccctgctctt cgccgctcac gcccaggaac
     1381 aggatggagc gggccgggcg ccgctccatc gacgcgaacg cctccgccac ctccagcagc
     1441 gccgaggtgc ccgaggcgtc gtcgtcggcg ccgttgtaga tgctgtcgcc ccgcaccggc
     1501 cggcccacgc ccacgtggtc gatgtgcgcg gagagcacga cgtactcgtt gcgcagcgcc
     1561 gggtcggcgc cggggaagat ggcgatcacg ttgggcgcca ccgccggccc gcgcccccgc
     1621 ggcgcgaagc gctggatgaa ctcgccgttc cccgccccgc cctgcagccc catccgctgg
     1681 aactcgcgca ccaggtacgc cgccgcggaa tccag
//
LOCUS       DATADN010000399         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_791901, whole genome shotgun sequence.
ACCESSION   DATADN010000399 DATADN010000000
VERSION     DATADN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791901"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VHH53_05230"
     CDS             <1..510
                     /locus_tag="VHH53_05230"
                     /inference="COORDINATES: protein motif:HMM:NF021000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2020 domain-containing protein"
                     /protein_id="HEX2289592.1"
                     /translation="GGLLAVATGSCGGFSTAPTLVAPVPADPSVAAPAAPPPPRPVAE
                     GQCDYLPAEAVEAVNGERVGAVQISADRPYPACFFLRSDGAVQLRSWIVVDTPEVARA
                     TVDAVAPVATSDLAELPGGWSGGSQPTADGAVFAVARHGTAVVVTTNQRQTISARRIA
                     GQVIATLGL"
     gene            complement(524..1006)
                     /locus_tag="VHH53_05235"
     CDS             complement(524..1006)
                     /locus_tag="VHH53_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289593.1"
                     /translation="MKTYARDGQQADAAVNSSVRAVRKRAARAGKQAKRIRKQLAEQL
                     PDRTAEISRGARSARRSAQEMLTERLLDARHELAARIEPTSGRRRRWPWMVLLLFGIA
                     GAAGAAVLTRRSQQTEPEGLPVHPARAANPPYNDEDVMGSSPTGNGVVSAGRPSSSAD
                     "
     gene            1128..1667
                     /locus_tag="VHH53_05240"
     CDS             1128..1667
                     /locus_tag="VHH53_05240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2289594.1"
                     /translation="MTEVKTNDTQVILHTSEGDIRVTLFPQHAPKTVSNFVGLAEGSK
                     EYSQRNAKGELSGPFYDGSLFHRVIDGFMIQGGDPTGTGRGGPGYRFADETHPELQFN
                     RPYLLAMANAGPNTNGSQFFITVGTAAHLNRKHTIFGEVADQDSRVVVDAIASTPTNG
                     DRPVNNIVVERVTVEPAGV"
     gene            1710..>1944
                     /locus_tag="VHH53_05245"
     CDS             1710..>1944
                     /locus_tag="VHH53_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2289595.1"
                     /translation="MTAPVCVRHPGRETSVRCTRCDRPACPTCLREASVGYQCVDCVA
                     EGARTQPRAATVAGAEPPGKSVVVPALIAINVVI"
BASE COUNT          297 a          664 c          656 g          327 t
ORIGIN      
        1 ggcgggctgc tggccgttgc aaccgggtcc tgtggtggct tcagcacagc tcccacgctg
       61 gtggcaccgg tgcccgcaga cccgtccgta gcggcaccag cggcaccacc cccaccgcgg
      121 cccgtcgcgg aagggcaatg cgactaccta ccggcagagg cggtggaagc ggtcaatggg
      181 gaacgagtcg gcgccgtgca gatctctgcg gaccggccgt acccggcgtg cttcttcctt
      241 cgcagcgacg gggcagtcca gctccgcagc tggatcgtgg tggacacccc ggaagtggcg
      301 cgggcaacgg ttgacgccgt cgctccggtg gccacctccg accttgccga gcttcccggt
      361 ggatggtcgg gagggtcgca gccgaccgca gacggagcgg tgttcgcggt agcccggcac
      421 ggcaccgcag tcgtcgtcac gactaaccaa cgtcagacga tcagcgcccg ccgaatcgcc
      481 gggcaggtga tcgctactct gggcctctga ctgacgagct cggtcagtcg gcgctggacg
      541 atgggcgacc ggcgctgacg acgccgttcc cagtgggaga actgcccatc acatcctcgt
      601 cgttgtaggg cgggttcgcc gctcgggccg ggtgaacggg gagcccctcg ggttcggtct
      661 gttgcgacct gcgggtcagc acggccgccc ctgccgcccc ggcgatcccg aacaacagca
      721 gcaccatcca cggccagcgc cgacgacgcc cagacgtggg ctcgatccgg gcagcgagtt
      781 cgtggcgggc atcgagcagc cgctcggtaa gcatttcctg agcgctgcgc cgggcgctgc
      841 gggcgcctct ggagatctcg gcggtgcggt caggtagttg ctcggcaagc tgtttccgga
      901 tccgcttagc ctgcttgccg gccctggcgg cacgcttgcg gacggcccgc acgctgctgt
      961 tcacagcggc gtcggcttgc tgcccatccc gtgcgtaggt cttcacggcc tttagctggt
     1021 tcacatcttc gccttcctgg tcgcgcctgc gtgcatgccc tcggaggcgg gcgccgccgt
     1081 ctgggccctc cccgcagtcg aacgtgggtg atggcaggat aggtccggtg actgaagtca
     1141 agaccaacga cacgcaggtc atcctgcaca ccagtgaggg tgacatccgc gtcaccctct
     1201 tcccgcagca cgccccgaag accgtgtcga acttcgtggg cctcgccgag ggcagcaagg
     1261 agtactccca gcgcaacgcc aagggagagc tgtccgggcc gttctatgac ggctcccttt
     1321 tccatcgagt gatcgatggc ttcatgatcc aagggggcga ccctactggt accggtaggg
     1381 gtgggcccgg ttaccgcttc gccgacgaga cccaccccga gttgcagttc aaccggccct
     1441 acctgctggc gatggccaat gcgggtccga ataccaatgg atcccagttc ttcatcacgg
     1501 tgggcaccgc ggcgcacctg aaccgcaagc acaccatttt cggcgaggtg gccgatcaag
     1561 actcccgtgt cgtggtcgac gcgatcgcca gcaccccgac caatggcgac cgtccggtga
     1621 acaacatcgt cgtcgaacgg gtgacagtcg agccggccgg ggtgtgacct ggtgaccgcc
     1681 gacgtgcttg gtcccgcgga ggcggcatca tgaccgctcc ggtgtgcgta cggcacccgg
     1741 gccgcgagac ctcggtgcgc tgcacgcgct gtgaccgtcc cgcctgccca acctgcctgc
     1801 gcgaggcgtc ggttggctac cagtgcgtgg attgtgttgc tgagggtgcc cgcacgcaac
     1861 cgcgcgccgc cactgttgcg ggagccgagc cacccggcaa gtcggtagtg gtcccagcgt
     1921 tgatcgcgat caacgtcgta atct
//
LOCUS       DATADN010000400         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_357294, whole genome shotgun sequence.
ACCESSION   DATADN010000400 DATADN010000000
VERSION     DATADN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357294"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VHH53_05250"
     CDS             <1..679
                     /locus_tag="VHH53_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2289596.1"
                     /translation="IGLLGDVQPRADRPYLVVKYAQSLDGRIATITGDSKWISGEEQR
                     SVSHALRAACDSVMVGVGTVLRDDPRLTVRLVPGASPLRVVLDSTLRIPSTALVLDGD
                     PVTVVFTTDRAAHSDRERLRALGVSVRTVPGSSAGVDLPATLRVLRELGVRTLLVEGG
                     ATMITSLLGDRLVDRLIVGTAPTILGAGTEAVGDLGIVRVTQGISLRNRCMHVTDYDV
                     ITAWDVA"
     gene            complement(676..1677)
                     /locus_tag="VHH53_05255"
     CDS             complement(676..1677)
                     /locus_tag="VHH53_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX2289597.1"
                     /translation="MDVDAMSWRLRTSEQRAMRAVVRRLPGSVADRLRLPPPDSTTVP
                     GRLRRAVLTVLRHGGIPASVQTFPLLDNPARWFVSAESLVLAQLYWFGEQGWEPELLP
                     WWRYLCRNSSAVLELGANVGYYVVQGAQAAPGVCYTAVEPHPDSLQLCRANLGLNGIM
                     SVTLVAAAAVADPAVDFVRLLVPRDQLPAPTVAFVDGDVELPPRMAGPVGAVIEVPAV
                     NVRTLLAGVDLLKVDVEGQEHALLAAASEHLREHRPTVVIEVLPGTTRLRRLLARLCT
                     ELGYRCYVPQPGRLKRLEPHRLEEVTLLHEYGVQDIVLSADPDLPLTVAELGPAGRC"
BASE COUNT          545 a          887 c          865 g          484 t
ORIGIN      
        1 tatcggcctg ctcggtgatg tacaaccgcg ggcggaccgc ccttacctgg tggtgaagta
       61 cgcccagtcc ctggacggac ggatcgcgac tatcaccggt gactcgaaat ggatcagcgg
      121 cgaggaacaa cgcagcgtct ctcacgccct gagggcggcc tgtgattcgg tcatggtggg
      181 ggtggggacg gtgctgcgcg acgacccgcg gctgaccgtg cgcctggtgc ctggtgcgtc
      241 gccgctgcgg gtggtcctcg actccacgct gcgaatcccc tcgacggcgc tggtgctcga
      301 cggagacccg gtcaccgtcg tgttcacgac cgaccgagct gcacacagcg atcgggagcg
      361 gctgcgggcg ctcggggtga gcgtgcggac ggtgccgggc agttcagccg gggtggacct
      421 gcccgcgacg ctgcgggtgc tgcgagagct cggtgtacgg acgttgctcg tcgagggtgg
      481 cgccacaatg atcacctcgc tgctcggtga tcgactggtc gaccgcctca tcgtcggcac
      541 cgccccgacg attctgggcg ctgggactga ggcggtgggc gacctcggaa tcgtccgggt
      601 gacccaaggc atttcgctgc gcaacagatg catgcacgtc accgactatg acgtcatcac
      661 cgcatgggac gttgcctaac acctgccggc cgggccgagt tcggcgaccg tcagcggcag
      721 gtcaggatcg gcagacaaga cgatgtcttg gacgccgtac tcgtggagca gcgtcacctc
      781 ttccaaccgg tggggctcca gccgcttcag ccgacccggc tgggggacat agcagcggta
      841 gcccagctcg gtgcacagcc gcgcgagcag tcggcgcaac cgcgttgtac ccggtaagac
      901 ctcgatgacc acagttggtc ggtgttcgcg caggtgctca ctggccgcag ccagtagcgc
      961 gtgttcctgg ccttcaacgt caaccttgag caggtctaca ccggcgagca acgtccgcac
     1021 gttgactgcc ggcacctcga tcaccgcccc gacgggaccg gccatccgcg gcggcagctc
     1081 tacgtcaccg tcgacgaacg cgacagtcgg tgcgggcagc tggtcgcgcg gaacaaggag
     1141 ccggacaaag tccaccgccg gatcggcgac tgcggcagct gcgaccagcg tgaccgacat
     1201 gatcccgttg agtccaagat tggcacggca cagctgcagg gagtccggat gtggctcgac
     1261 tgccgtgtaa cagacacccg gcgccgcctg cgccccctgc acgacgtagt acccgacgtt
     1321 agcccccaac tccaagaccg ccgacgagtt gcggcacaga taccgccacc aaggaagcag
     1381 ctcgggctcc cagccctgct caccaaacca gtacaactgg gcaagaacca acgactccgc
     1441 gctgacaaac cagcgtgccg ggttatccaa caatgggaat gtctggaccg acgcggggat
     1501 gccgccgtgc cgcagcacgg tcaacacggc acggcgcaga cggcccggca cagtggtgga
     1561 gtccggcggc ggaaggcgca gccggtcggc tacggagcca ggtagccggc gcaccacagc
     1621 gcgcattgcc cgctgctccg acgtccgcag ccgccacgac atcgcatcga cgtccacccg
     1681 cgcatgcctc ccctcattcc caccaatcac acacggcgac gcgtccgcct cccacctctc
     1741 agttgaggag atcttggcta taacgcgcca ggccaaagtg accccacgag gacaaccgcc
     1801 actaaacatc aacggagatc gagatactgt tggtggctac gccacggtag aagcgtgaca
     1861 ccaggcaacc cgtggacgca tgtcccctgg tgcgactgaa acactccccc gatcgggtat
     1921 ccggtgctgt gtcgattcgc tggcgtgatc aggtctgtgt agatcatttg ctggagtggg
     1981 cgctggcggc gctagcgaag ctaatcagtg tctggcccag cggccagggc ctcacgaggt
     2041 tcacactgtt acaccaaggg agacgtgagg gcaggacatg gaacaggaac atgtcgcaaa
     2101 agatgcgcct atgagcgagc gcccatgggg actgccacgt gctcttgcga gcactgtgca
     2161 acgttacatc cgatacgagg tcgcagtaga aacagggaca tatgaaggtg caagtactcg
     2221 aatattacga gaattggtcg gcccagtata caccatagag gcaaactttg gcctctatga
     2281 aaagtctcgt catgccctgg cgaacgatcc tgacattcat gtagttttcg gatcgtcacc
     2341 cgtcgtgctc ccatctatcc tcgactcctt ggactcgtgt gcgctgtttt ggctcgacgc
     2401 ccactggttc cccgaggtcc ctgaagttac cgcgtctcaa tgccccttgc tggaagagct
     2461 ttctatccta gcgaagtggc ccctcatcgg tggctcctgc gtgcttatcg atgatgcccg
     2521 tatgtttagt aaaccgcttg ataaatgtta tcggccgagc gactggccaa cgttccctga
     2581 gattgctaag actgcgcgat tcacaccgtc gaccgtgatg acagttattg aggatgtaat
     2641 agtcattggc cccagtacgc tcacggaggc gtttggcacc tacatggcga ggcatggaac
     2701 gcaaagcccc gtatggaaat gaaccatgag gggactcacc aggcccccgc caccgtgaag
     2761 atcaacggcg ccatggtgct t
//
LOCUS       DATADN010000401         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1110128, whole genome shotgun sequence.
ACCESSION   DATADN010000401 DATADN010000000
VERSION     DATADN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110128"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(635..850)
                     /locus_tag="VHH53_05260"
     CDS             complement(635..850)
                     /locus_tag="VHH53_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289598.1"
                     /translation="MDESIMRCDNERARVVVEGRLTDGGGLCTLVAVRYRSPRCWILY
                     PHGASKLGVVLGEQDAAILAQGIAESG"
     gene            1030..>1523
                     /locus_tag="VHH53_05265"
     CDS             1030..>1523
                     /locus_tag="VHH53_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2289599.1"
                     /translation="MSKQPVGVGQRVAERRKLNGLTQAQLAHRAHVSLSLVRKVEQGS
                     APASPAFTAAVATALRTTVAELYDQPSLRYGAERDHIAEVETAVMEGPASVTAYRPPD
                     LDVLAARVDEIAKLQRRSRYHESSALMPSLLAELHTTAALAPAGAHSERAHHLLATLY
                     GCVV"
BASE COUNT          302 a          509 c          497 g          215 t
ORIGIN      
        1 gaactcggcc aggtagcagg ccagcgcgag gacaacctga ccggcggcgc gccgccgatc
       61 ctccaggcgc aagaacgccg ccgcaccggc agcactacca acagcagcag tgccgatgac
      121 gcaggggtac agcgagatgg cggtcatggt ggttctccct tgcttaagcg gggtgggaca
      181 tagcggcgcc tccacccgcg tgcgaacgtg acggtgtggg gctgtgacaa cggaccgacc
      241 acggggcggc cgggaagagc cgcgcggtgc ggcgacaaga agaaggcccg gagcggtctg
      301 ccgcaaagcc gggtacgtca tgcagcggcg gatgttcgct gcgcggctac tgcgcgccga
      361 tcacgccacg acgtctcaac agccccgctc aacggggtaa ataccgtgcc gatacggcag
      421 gcatgacaca gagtacgacg gttcggtgac ctgaagtgac aataaagtgc caatacgtca
      481 ctgcccgcgt acaacccggt tggggcgtcc acgaagtacc cgagtgctat gagggggctg
      541 ggcgacggtg acgggtcagc tcagtgccac cacagtcgac cgcatggggt cgccgtcgag
      601 tgggtacacg cccaaggcac ggtagggcag ttcatcagcc gctctcggcg atgccctgag
      661 cgaggatggc cgcgtcctgc tcgccgagca cgactccgag tttgcttgca ccgtgcggat
      721 acagaatcca gcaccgcggg gagcggtaac gcactgccac cagcgtgcac aggccgcccc
      781 catcggtgag ccgaccctcc accaccaccc gagcacgctc gttgtcgcac cgcatgatcg
      841 attcgtccat cgtcccctgc gctcaacctg taggcactgc ccggaccagc cggacatggc
      901 cgcggaccag cgaagatcac taaaccgacg gtagggtgat ggcagtggcc gaactgacca
      961 agcagtgaca agcagtaacc ctcgacagcc gccgatagca ttcgaggcta accccagcgg
     1021 gaggtggact tgtcgaaaca gccggtcggc gttggtcaac gtgtcgccga gcggcgcaag
     1081 ctcaacggcc tcacccaggc ccagcttgcc caccgggcac atgtgtcgct gtccctggta
     1141 cgcaaagtgg aacagggcag tgcaccggca tccccggcgt tcacggcagc ggtggccacg
     1201 gcgctacgca cgacagtcgc cgagctgtat gaccaaccca gcctgcgtta tggcgccgaa
     1261 cgtgaccaca tagctgaagt cgaaaccgcg gtcatggagg gtccggcgag cgtgacggcg
     1321 taccgcccgc ccgatctgga cgtgctcgcg gcccgggtgg acgagatcgc taagttgcag
     1381 cgacgcagcc gctaccacga atcctcggca ctgatgccgt cactgttggc cgagctgcac
     1441 accaccgccg ccctggcacc cgcgggcgca cacagcgagc gcgctcatca cctgctggcc
     1501 accctttatg gctgcgtggt gat
//
LOCUS       DATADN010000402         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1410438, whole genome shotgun sequence.
ACCESSION   DATADN010000402 DATADN010000000
VERSION     DATADN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410438"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..1242
                     /locus_tag="VHH53_05270"
     CDS             592..1242
                     /locus_tag="VHH53_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289600.1"
                     /translation="MSGTAALAARYRPGADADATQPVHLVPLPVRTAVGAVSAFCGTL
                     LSMPKLEAVESGQGVPCPMCVLQHGATTQAPQNETDYSSLDWPVQLQGDQLVLPLGHC
                     ATAHIVPMDLVAAITLILAALDRPVPVLIHPDAPGCGVVIAGEPYGVPLPWPATVQVV
                     TGMLPLPPSQTPHGPVHWYQHQPHHPLATCREIDVFSAMRTAGLAAHEQSGTHRAR"
     gene            complement(1227..>1571)
                     /locus_tag="VHH53_05275"
     CDS             complement(1227..>1571)
                     /locus_tag="VHH53_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX2289601.1"
                     /translation="AAAAVAHRLEGAVALGGKAGRDLLCKVPGIGVWTAAEVAQRAWG
                     DADAVSVGDFHIPSVVGYALLGRPLDDEGMLDVLAPYLPQRHRAVRYVEASGFRRPRF
                     GPRFSPRDYRAL"
BASE COUNT          287 a          549 c          504 g          231 t
ORIGIN      
        1 tcgacgacct caccagctga gtcgaccagc gccgaggtca agcaactagt ccaccgacag
       61 ttcgaagcca ggacgcatgg aagatgcacg aaccgacgac ccgagcgaag ttcatgacgt
      121 ggttcacctg cgcattcgac cgtaccgacc atgcgatcag tgatgacaac atggcaatcg
      181 ggttttcggt gggttcagga cgatatgccg cgctctgcgg tactacggtg tgtgtcgcat
      241 ccatgatgag cccaccaggt cgtcgttgtc cgtcatgcga aacgatcgtg cagcggcttg
      301 accgtgaacc gttgcctagc aaggccggag tactcgcccg ctatttcggg cgtcgacggc
      361 accgcgacgg cacaacaggc ctatgaacca gcagggttat tctcggcacg ggcaacgttc
      421 cagcaacccc cgccccgggg gctcgcactg gcccggcggc caactcaagc cacggcggag
      481 ccggggagca tcacgaatgc ccccggctcc gccgtgcccc acaaccacac cgaatgacca
      541 gactccaccc cgctgtgcca ggtcagtgat attcacagcg gcggggcaag cgtgagcggc
      601 actgcggcgc tggccgcgcg ttaccggccc ggggcggatg cagacgcaac gcagcccgta
      661 catctggtcc cattaccggt ccggaccgcg gtcggcgcgg tcagcgcgtt ctgtgggacg
      721 ttgctgtcca tgccgaagct ggaagccgtc gaatcgggtc agggcgtgcc gtgcccgatg
      781 tgtgtgctgc agcacggcgc cacgacgcag gccccgcaaa acgagacgga ctacagctcg
      841 ctggactggc cggtgcagct gcagggcgat cagctagtgc taccgttggg gcactgcgcc
      901 acggcacaca tcgtgccaat ggatctggtc gcggcaatca cactgatcct ggccgcactt
      961 gatcggccgg tgccggtcct gatccacccg gacgctccgg ggtgcggcgt ggtcatcgca
     1021 ggtgagccct acggcgtgcc gctgccctgg cccgcaacgg tgcaggtggt caccgggatg
     1081 ctgccgctac cccccagcca gaccccacac gggccggtgc actggtatca acaccaacca
     1141 caccatccgc tggccacctg ccgcgaaatc gacgtcttct cagcgatgcg caccgctggg
     1201 ctggccgcgc acgagcagag cggaactcac agggcgcggt agtcgcgagg actgaaccgg
     1261 gggccgaagc gggggcggcg gaacccggag gcctcgacat agcgcaccgc tcggtgccgc
     1321 tgcggcaggt agggcgccag aacatcgagc attccctcgt cgtccaatgg ccggcccagc
     1381 agggcgtaac cgacgacgga gggaatgtgg aagtcgccga cgctcaccgc atcggcgtct
     1441 ccccaggccc tttgggccac ctcggcggcc gtccagaccc cgattcctgg gaccttgcac
     1501 agcagatcac gaccggcctt accgcccagt gccaccgcgc cctccagccg gtgcgccacc
     1561 gcagccgccg c
//
LOCUS       DATADN010000403         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_504320, whole genome shotgun sequence.
ACCESSION   DATADN010000403 DATADN010000000
VERSION     DATADN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504320"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VHH53_05280"
     CDS             <1..664
                     /locus_tag="VHH53_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2289602.1"
                     /translation="MVHEDNAVTALCEAVARLGKHRFPLVMTDTVQEFLAAIGEETGL
                     DFSGDDLEGAVAKLGGLARIIGATLRDTANPTMLDAGYKANVIPSTAEAVVDCRVLPG
                     RQVAFEREVDKLLGPDVTREWVMNAPAVETTFDGDLVAAMDAAILQEDPGARTIPYML
                     FGGTDAKAFSRLGMRCFGFAPLRLPPDLDFAAMFHGVDERVPVDGLSFGARVLDRFLR
                     SC"
     gene            894..1097
                     /locus_tag="VHH53_05285"
     CDS             894..1097
                     /locus_tag="VHH53_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2289603.1"
                     /translation="MATGTVKWFNSEKGYGFITQDGGGPDVFAHFSAIQATGYRTLEE
                     NQRVEFEITQGPKGPQADQIRTI"
     gene            complement(1156..1374)
                     /locus_tag="VHH53_05290"
     CDS             complement(1156..1374)
                     /locus_tag="VHH53_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEX2289604.1"
                     /translation="MIETVDGDGLDWEYQPLRLPPGVSQLTAATQLAVQAEFAGWELS
                     RTRMYSDGTRTVWLRRRRTAAAQPKLSW"
     gene            complement(1374..>2043)
                     /locus_tag="VHH53_05295"
     CDS             complement(1374..>2043)
                     /locus_tag="VHH53_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289605.1"
                     /translation="LLVPVTTPGSLVRVPLEEGPPSTTNLDGPAYGALAMGQTTVVAM
                     GDRLTVLDGSSSRTIPGFVDATRLVPLGAKVGVVDRGRSAVSLVDISADDAGLALRAG
                     NGAAAAVSDRFGRMLVTDVRDGELLVFAGDPLLLRQRFPLPGAPYGVAYDPRRDLAWV
                     TLTARNEVVGLDVAGGEPVVVHRFPTVHQPDAVAVDPASGRVVVVSASGAGLQVIDPE
                     RVVR"
BASE COUNT          340 a          701 c          685 g          317 t
ORIGIN      
        1 gatggtgcat gaggataatg cggtgaccgc attgtgcgag gcggtggccc ggctggggaa
       61 gcaccgtttc cccctggtga tgactgacac ggtgcaggag ttcctggcgg ccatcggcga
      121 ggagaccggg ctggacttct ccggcgacga tctggagggt gccgtcgcca agctcggcgg
      181 gctggcccgg atcatcggcg cgacgcttcg cgacaccgcc aacccgacaa tgctcgacgc
      241 cgggtacaag gccaacgtca tcccctcgac ggcggaggcg gtcgtggact gccgggtgct
      301 gcccgggcgc caggtggcgt tcgagcgtga ggtcgacaag ctgctcggcc ccgacgtcac
      361 ccgcgagtgg gtgatgaacg ccccagcggt ggagacgacc ttcgacggcg atctggtcgc
      421 cgcgatggac gcggcgatcc tgcaggagga tcccggtgcg cgcacgatcc cctacatgct
      481 cttcggtggt accgatgcga aggcgttcag ccggctggga atgcgctgct tcggcttcgc
      541 gccgttgcgg ctgccgcctg atcttgattt cgccgcaatg ttccacgggg tcgacgagcg
      601 ggtgccggtc gatgggctct ccttcggtgc ccgagtcctt gaccgattcc tccgtagctg
      661 ctgagggggc cagcccgggg ggtcgttaag ttacccctac cgcggcagac acattctgct
      721 aacgtctgca cccgtagtag tcttggttcc taggacgcct gtggagcaag cgccacaggc
      781 gttcgttgtg tccggccagc gcagtgcgtg cacagcgccc cgggtgatca cgacagcgaa
      841 ttcgggacac ccgcattacg ggccgcacag gcggcccgag aaggagatga aagatggcga
      901 ccggcacggt caagtggttc aactcggaga aggggtacgg cttcatcacc caagatggtg
      961 gcggccccga tgttttcgcc cacttctcgg cgatccaggc gaccggctac cgcacgctgg
     1021 aggagaacca gcgggtggag ttcgagatca cccagggacc gaagggtccg caggccgacc
     1081 agatccgcac gatctaacga cctgctcact cgtagggtcc gcaccgcacg gtgcgggccc
     1141 tacgtcattg cggacctacc acgacagctt gggctgcgcg gccgcggttc gtcgcctgcg
     1201 cagccacacg gtgcgcgtgc cgtccgagta catccgcgtc cgggacagct cccagcctgc
     1261 gaactcggcc tgcactgcga gctgggtagc cgcggtcagc tgggataccc ccggcggtaa
     1321 ccgcagcggc tggtactccc agtcgaggcc atcaccgtca accgtctcga tcatcatcgc
     1381 accacccgtt ccgggtcgat cacctgcaat cctgcgcccg atgcggagac gacgaccaca
     1441 cggccgctgg ccgggtcgac tgctaccgca tcgggttggt gcacagtagg gaaccggtgg
     1501 acgaccaccg gctcgccccc tgccacatcc agccccacca cctcattgcg ggcggtcagg
     1561 gtcacccacg ccaggtcccg ccgggggtcg taggccaccc cgtacggcgc gcccggcagg
     1621 ggaaaacgtt gccgcaacag cagcgggtcg ccggcgaaaa ccagcaactc accatcgcgg
     1681 acgtcagtca ccagcatccg gccgaaccgg tcggacaccg cagcagctgc tccgttgccc
     1741 gcccgcagcg ctaggccggc gtcgtcggcg gagatgtcca ccaggctcac cgcactgcga
     1801 cctcgatcca caacaccgac tttggcgcca aggggcacca gacgggtggc gtcgacgaac
     1861 cccgggatgg tgcggctcga gctgccgtcg agcacggtca gccggtctcc catcgcgacc
     1921 acggtggtct gacccatcgc gagcgccccg taagccgggc cgtcgagatt cgtcgtgctc
     1981 ggtggcccct cctcgagcgg gacccgcacc agtgatccgg gcgtggtgac cggcaccagc
     2041 agc
//
LOCUS       DATADN010000404         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_960784, whole genome shotgun sequence.
ACCESSION   DATADN010000404 DATADN010000000
VERSION     DATADN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960784"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..548)
                     /locus_tag="VHH53_05300"
     CDS             complement(186..548)
                     /locus_tag="VHH53_05300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2289606.1"
                     /translation="MAALPLPADAAAAPLVRFLGLRTASVLGALDRMEERHPRDPSHW
                     YLFILGTEQAAQGQGLGSALLAQVLARVDADGMPAYLESSSERNLALYGRHGFEITGE
                     VAIPGGPRIWPMWREPRT"
     gene            complement(994..1869)
                     /locus_tag="VHH53_05305"
     CDS             complement(994..1869)
                     /locus_tag="VHH53_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2289607.1"
                     /translation="MNDRASPAVVDADLLLADGRRLGYAVWGAPEGQAALLFHGSPGS
                     RLFCPDPAATAAAGVRLVTVDRPGYGRSAAAPGRRIMDWPTDIEQLVTALGIDRFALI
                     GHSSGGPYALACALAMPQRITRMALVSCVVPLDEVPAAWAALDDDERRLVELARHHPD
                     QAAATIADAAGWLADQPDRFLTLPRPEPDALLLQDPSVRSRYLDMVREAVRQGLAGYL
                     SDEILERRPWGFQLGDVNSPVAVWHGGKDGYIPLAHVEAMAALLPRSRTRFHADQAHG
                     LIIASWDAILAELTS"
     gene            complement(2238..2474)
                     /locus_tag="VHH53_05310"
     CDS             complement(2238..2474)
                     /locus_tag="VHH53_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289608.1"
                     /translation="MEPSTATAQLPGQVWRRIVIVAALVSALVGGVVAVAERTMASEP
                     GAGDVRVQAPWADPSCRELIAVPKRGWVCVDRRP"
     gene            complement(2524..>3302)
                     /locus_tag="VHH53_05315"
     CDS             complement(2524..>3302)
                     /locus_tag="VHH53_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2289609.1"
                     /translation="QCLRNEDILDASCHRAVLGFLELSLPNPSAAIERLRPVLAFLDA
                     LGSPEPGIIPCLPDAVEALVSLGRLDEAEALLNRLERQGRALDRPWAVATAGRGRGLL
                     TAARGDPATARSALEQALVDHGRVPQPFELARTLLVKGEVERRAKQKRAARATLEQAL
                     GLFQALGAPLWARRARDDLARVGGAMPPSSELTPTEQRIAQLVGEGKKNREIAEALFI
                     SVKTVEANLSRIFHKLGVRSRTELAALQRVSPDSSPVHRT"
BASE COUNT          534 a         1154 c         1135 g          479 t
ORIGIN      
        1 ccaaagaatg ggatagcaac cgggccgaac cagagcgaag ggcgcattcc tggttgctca
       61 cgtcgcttcg gcgacgatga aacattgccg cccggcgagc gggtgatgac cggtaaagcg
      121 ccatccagtg tggtccagcc agccgcggac ctcgtccacg ctgtagacat cgccggtgca
      181 cccgctcaag ttcggggctc gcgccacatg ggccagatcc ttgggccgcc cgggatcgcc
      241 acctcgccgg tgatctcgaa gccgtggcgg ccatacaagg cgaggttccg ctcgctcgag
      301 gactccaggt aggcaggcat cccgtcggcg tcgacgcggg ccagcacctg ggccagcagg
      361 gccgagccta acccttgtcc ctgggccgcc tgctcggtgc ccaggatgaa caggtaccag
      421 tgggacggat cccgcgggtg ccgctcctcc atgcggtcga gtgcacccaa caccgaggcg
      481 gtccgcagac caaggaagcg gaccaggggg gcagcagccg catccgctgg gagcgggaga
      541 gctgccatct cccgggcggc gggagccaga cagcggccgc ggccccgccg gcggtcatcc
      601 acacttcgtc gtgggggaag gccagcttgc gtaggcgtaa ctccatgaag gctggcagga
      661 cacgaccgcg gcgttgctcg tccgggaaga tccaggcgat gaccgggtcg tcctggaagg
      721 ctgacgccag cgcctgggcc agccgagggg catcggccag ggttgctttg cgaaccgacg
      781 acccagtcgg catggccggc ttccctcgcc tgagaccaac aggacctctg tgagctatcg
      841 tagccgccac ctggtggttc aggtccaggt ccaagcgttt ggccccggcc accatgatgg
      901 actgcacagc agggtcgcgg ttcaccccat ccttgtagga cttacggagt cgcccgacca
      961 ccctcagccc cctgcccctg ctgagttcgc cggctagctg gtcagctcgg ccaggatggc
     1021 atcccaactt gcgatgatca gtccgtgtgc ctggtcggcg tggaacctgg tgcgcgatcg
     1081 gggtagcaac gcggccatgg cctcgacgtg ggcgagtgga atgtagccgt ctttgccacc
     1141 gtgccagacc gccaccgggc tgttgacatc gccgagttgg aatccccagg gtcgacgctc
     1201 caggatctcg tcggagagat atcccgccag cccctggcgt accgcctccc ggaccatgtc
     1261 gagatacctc gaccggaccg aggggtcctg cagcaacagc gcgtcaggct ccgggcgggg
     1321 cagggtcagg aatcggtcgg gttggtcggc aagccacccg gccgcgtcag cgatggttgc
     1381 cgcagcctgg tcggggtggt gccgggccag ctcgaccagc cgccgctcgt cgtcatcaag
     1441 ggcggcccac gccgcgggta cctcgtcgag tggcacgacg cagctgacca gggccatgcg
     1501 ggtgatccgc tggggcatcg ccagggcgca ggccagcgcg tagggcccac cggaggaatg
     1561 cccgatcagc gcaaagcggt cgatacccag cgcagtgacc agctgctcga tgtctgtggg
     1621 ccagtccatg atgcggcgtc ccggcgcggc agccgagcgt ccgtagccgg gccggtccac
     1681 ggtgaccagc cgaacgccgg ctgcggcggt ggccgccgga tcggggcaga acagccgcga
     1741 cccgggagat ccgtgaaaca gcagcgccgc ctgtccttcc ggcgctcccc acaccgcata
     1801 gccgagtcga cggccatcgg ccagcagcag atcggcatcg acaacagcag gcgaggcccg
     1861 gtcattcatg acaaccacta tcctccgcga cggacttgcc accgtcgagc tcgtgagctt
     1921 gtggacggcg aagaacgcgc tcgttggacg cgatccgcca ccagctgcgc gacagaccca
     1981 gccgcagaag cgccccgacg gctggcaact ggttccctcg agtcaccgac ggcaccatcg
     2041 taggcagggc cgtgcggccg ccagcagccg ccagtcgccg tcgatccctt cagcaggtac
     2101 atgccggggt cctcgagggt gacgtcggat ccggcggcca ggtcccgcac ggtcctggac
     2161 cgctacgagg gccagggtcg ggtcctcgcg gtcatcatta gcacttcctc cggacccatc
     2221 catcgcagcg atgtgcctca gggtcggcga tccacgcaga cccagccccg cttcgggacg
     2281 gcgatgagct cccggcacga tggatctgcc caaggtgcct gaacccgcac gtcaccggcc
     2341 cccggctcgg acgccatcgt gcgctcggcc acggcgacca cgccgcccac cagggcagaa
     2401 accaaggccg cgacaatcac gatgcgccgc cagacctgcc cggggagctg tgcggttgct
     2461 gtgctcggct ccatgtcgac cctcctcctt gctgcgggcg ttgggacagc cctcggcagc
     2521 aggctaggtc ctgtggaccg gtgaggaatc gggggaaacc ctctgaagcg cagcgagctc
     2581 tgtgcgcgaa cgtaccccga gcttgtggaa gatccgggac aggttcgcct cgaccgtctt
     2641 cacggagatg aacagggcct cggcgatctc acggttcttc ttcccttccc cgacgagctg
     2701 ggcgatccgt tgctcggtgg gggtgagctc actggacggc ggcatcgcac cgccgacacg
     2761 ggcgaggtcg tctctggccc tccgcgccca caacggcgca cccagggcct ggaagaggcc
     2821 aagcgcctgc tccagggtgg cccgggcggc tcgcttctgc ttggctctgc gctccacctc
     2881 gcctttcacc agcagggtcc gggccagctc gaagggctgc ggcacacgtc cgtggtcgac
     2941 cagggcctgc tcgagcgccg agcgtgccgt ggccggatcg ccccgggccg cggtgagcag
     3001 cccacggccc cgcccggcgg tggcgacggc ccacggccga tcgagggctc gtccctggcg
     3061 ctccagacgg ttgaggagcg cttcggcctc atccagccta cccagggaga ccagggcctc
     3121 gacggcgtct ggcaggcagg ggatgatgcc gggctccggg gaccccagcg cgtccaggaa
     3181 cgccagcacc ggccggagtc gttcgatcgc cgcgctgggg ttgggcaggg agagctcgag
     3241 gaacccgagc accgcccggt ggcagctcgc gtcgaggatg tcctcgttcc gcaggcattg
     3301 gc
//
LOCUS       DATADN010000405         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_358140, whole genome shotgun sequence.
ACCESSION   DATADN010000405 DATADN010000000
VERSION     DATADN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358140"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VHH53_05320"
     CDS             complement(<1..839)
                     /locus_tag="VHH53_05320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2289610.1"
                     /translation="MNVSQDAASFADLALRPELLCVLSGLGYEEPTPIQREAIPPLVQ
                     GRDLLGQAATGTGKTAAFALPVLQRMPGDGRGAGPVALVLVPTRELAMQVSQAFHRYG
                     RDLGVRVLPIYGGQPIGRQLQVLERGVDVVVATPGRAIDHIGRGTLRLRGVETVVLDE
                     ADEMLDMGFAEDIEAILDGTPEDRQTVLFSATMPSRVDGIARRHLRDPVRIEMGRAAP
                     AAGTGPLVRQSAYVVARAQKPAALGRVLDVETPSAAIGFCRTREEVDQLTEILNGRGY
                     RAEA"
     gene            complement(917..>1839)
                     /locus_tag="VHH53_05325"
     CDS             complement(917..>1839)
                     /locus_tag="VHH53_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2289611.1"
                     /translation="RVGEYWLRKGIDGWRLDVPSEIKSDGFWQEFRQRIKAINPEAYI
                     VGEVWGDSRQWLRGDQFDGVMNYLFAEAAIAFSAGHRVRPETVEGRSYAPWPGIDGRA
                     YAAKIDHLLAMYPWPVQLTQLNLLDSHDTSRFVTIAGDDRASVRLGTLLLFTFPGAPS
                     IYYGDEIGLGGALPDHWARKSFPWNHPQRWDLDALAFHKAVIALRQSHPAFQTGDYIP
                     LYADPDVYAFARTLQGSIMITAVNVAGASRRFTIPLISALPAHPATVTILLTTDGSPI
                     AELAPGTLTLALPARSGAVFDASPDSRNRA"
BASE COUNT          325 a          625 c          610 g          279 t
ORIGIN      
        1 gcctcagcgc ggtagccgcg accgttcaag atctcagtga gctgatcgac ctcttcccgg
       61 gtccggcaga aaccgatcgc agccgagggc gtctccacat ccagcacccg tccaagggca
      121 gcgggctttt gcgcacgagc gaccacgtac gcgctctgcc gcaccagcgg gcccgtaccc
      181 gcggccggcg ccgcgcggcc catctcgatc cgcaccggat cacgcaggtg gcggcgggcg
      241 atcccgtcga cacgagaggg catggtcgcg gagaacagca cggtctgccg atcctcagga
      301 gtgccgtcga ggatcgcctc gatgtcctca gcgaacccca tatcgagcat ctcgtccgcc
      361 tcatccagca cgaccgtctc cacaccgcgc aggcgaagcg tcccacgacc gatatggtcg
      421 atcgcgcggc caggcgtggc gaccacgaca tcgaccccgc gttccagcac ctgcaattgc
      481 cggccgatcg gctgcccccc atagatcgga agaacgcgga caccaagatc gcggccgtag
      541 cggtggaacg cctgggagac ctgcatggcc agctcacgcg tgggtaccag caccagagcc
      601 accggccccg cacctcgccc gtcaccgggc atccgctgca acaccggcaa cgcgaacgct
      661 gccgtctttc ccgttcccgt cgcggcctga ccgagcaggt cacgcccctg caccaacggt
      721 gggatcgcct cgcgctggat cggcgtgggc tcctcatacc caagccccga cagcacacag
      781 agcaactcag gccgcagcgc cagatcagcg aagctcgccg cgtcctggga cacgttcaca
      841 cccatcctcg ccctcctggt cctactgccg tccgtccatg ctccacctac ccgccggacc
      901 agggcgcgac tgggtctcag gcgcgattgc gggagtctgg cgaggcgtcg aagaccgcgc
      961 cgctgcgtgc tgggagagcc agcgtcagcg tgccgggtgc tagctcggcg atcggtgagc
     1021 cgtcggtcgt caagagaatc gtgaccgtgg ctgggtgcgc cggtagcgcg ctgatcagcg
     1081 gaatcgtgaa ccgacgcgac gccccagcga cattgacggc agtgatcatt atgctgccct
     1141 gcagggtacg ggcgaaggca tagacgtccg gatcggcgta gagcgggatg tagtcgccgg
     1201 tctgaaaagc ggggtgggac tggcgcagcg caatgaccgc cttgtgaaag gcgagcgcgt
     1261 cgagatccca ccgctgcggg tggttccatg gaaaggactt gcgcgcccag tggtctggca
     1321 gtgccccgcc cagaccgatc tcgtcgccgt agtagatcga gggggcgccg gggaaggtga
     1381 acagaagcag cgtaccgagc cggacgctgg cccggtcgtc gccggcgatg gtgacgaagc
     1441 gggaggtgtc gtgactgtcg agcaggttga gctgggtgag ctggacgggc cagggataca
     1501 tggccaggag gtgatcgatc ttggccgcgt aggcgcggcc atcgatgccg ggccagggag
     1561 cgtatgagcg accttcgacg gtctcgggtc gcacccggtg gccggcgctg aaggcgatcg
     1621 ccgcttcggc gaagagatag ttcatgacgc cgtcgaattg gtcgcctcga agccattgcc
     1681 ggctgtctcc ccagacctca ccgacgatgt acgcctcggg gttgatcgcc tttattcgct
     1741 ggcggaactc ctgccagaag ccgtccgact tgatttcgga cgggacatcg agccgccagc
     1801 catcgatccc cttgcgaagc cagtactcgc cgacgcgca
//
LOCUS       DATADN010000406         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_504726, whole genome shotgun sequence.
ACCESSION   DATADN010000406 DATADN010000000
VERSION     DATADN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504726"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VHH53_05330"
     CDS             complement(<1..1101)
                     /locus_tag="VHH53_05330"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX2289612.1"
                     /translation="MGRPGFVLDVDERTPPLLMHEGEGFRLERLPLGSRVIYPPESLP
                     GVVDLEGTIRHALAHPHGSDPLPELLRPGMRLTIVFDDLSLPLPPMRTPDVRQRVIEQ
                     VLEMAAAAGVDDVELIAANSLHRRMTPAELQRTVGERVFRAFHPDHLRNHDAEDPDDL
                     AHLGTTPHGEDVEINRRAAESDLIVYVNITLTAMSGGTKSVAVGLASYASLRHHHNVH
                     TLRHSRSFNDPPNSAMHHSYDRMADVIGGHVPVFTIETTLNNEPFPSSLRFLTRREWE
                     WNLTDQASYLAVKRSNELAPTKLRRRIWQATEAPYGVTGVHAGTPSQVHPRTLAAVHR
                     QQLTEVQGQADVAIFGLPYICPYNVNSIMNPIL"
     gene            complement(1140..>1530)
                     /locus_tag="VHH53_05335"
     CDS             complement(1140..>1530)
                     /locus_tag="VHH53_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2289613.1"
                     /translation="LLVEPEFGAGYLLGGMDVAFECTGAAAGLDTALRSLRAGGTVIA
                     SGMPSGGVDLTPLWYRELHLVGAYASAVGDEPDGGDFPEAIALAGSAPLDGYVDTKYP
                     LERWRDALDHAAAAGRLGTVKVVFAPS"
BASE COUNT          240 a          504 c          521 g          265 t
ORIGIN      
        1 caggatcggg ttcatgatcg agttgacgtt gtagggacag atgtagggca agccgaagat
       61 cgccacgtca gcctgccctt gcacctcggt gagctgctgg cggtgcaccg ctgccaacgt
      121 gcgcgggtgc acctggctcg gtgtgccggc gtgcaccccg gtgacgccat acggcgcttc
      181 ggttgcctgc cagatccgtc gccgcagctt ggtgggggcc agctcattgc tccgcttcac
      241 cgcgaggtag ctggcttgat cggtgaggtt ccactcccac tcgcgacggg tgaggaagcg
      301 cagcgagctg ggaaacggtt cgttgttcag ggtcgtctcg atggtgaaca ccggcacgtg
      361 cccgccgatg acatcggcca tccggtcata ggagtggtgc atcgccgagt tgggcggatc
      421 gttgaacgag cgcgagtgcc gcagcgtgtg cacattgtgg tggtggcgca ggctggcgta
      481 actggccagc ccgaccgcca ccgacttcgt gccgccgctc attgcggtga gcgtgatgtt
      541 gacgtagacg atcaggtcac tctccgcggc ccggcggttg atctccacgt cctcgccgtg
      601 cggtgtcgtg cccaggtgcg ccaggtcatc gggatcctcg gcgtcgtggt tgcgtaggtg
      661 atcggggtga aaggcacgga agacccgctc gccgacggtg cgctgcaact ccgccggagt
      721 catccgccgg tgcagcgaat tggcggcgat gagttcgacg tcgtccacac cggcggcagc
      781 ggccatttcc aggacttgct cgatcactcg ctggcggaca tccggtgtcc gcatcggcgg
      841 cagcggtagc gacaggtcgt cgaacacgat ggtcagacgc atccctgggc gcagcaactc
      901 gggcagcggg tcgctgccgt gcgggtgtgc caaggcgtga cggatcgtgc cttcgaggtc
      961 gacgacgcca ggcagcgatt ccggcgggta gatcacgcgg ctgcccagcg gcaggcgctc
     1021 cagccggaat ccctcaccct cgtgcatcag caggggggga gtccgctcat cgacgtcgag
     1081 cacgaaacct ggtctgccca cattctcacc tgctcctgtc cgcggcgttg cgcttaacct
     1141 cagctgggcg caaagacgac cttgaccgtg cccagccggc cggccgcggc ggcgtgatcg
     1201 agcgcgtcac gccaacgttc cagcgggtac ttggtatcaa cgtaaccgtc cagtggtgcc
     1261 gatcccgcca gcgcaatagc ttcggggaaa tcccccccgt cgggctcgtc gcctactgcc
     1321 gaggcgtagg ccccgaccag gtgcagctcg cggtaccaca gtggggtgag gtccacccca
     1381 cctgagggca ttcccgatgc gatcaccgtg cccccggcac gcagcgaccg caacgcggtg
     1441 tccagtcccg ccgctgcccc ggtgcactcg aaggccacgt ccatgccgcc gagcaggtag
     1501 cccgcgccga attcgggctc gaccagcagt
//
LOCUS       DATADN010000407         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_504768, whole genome shotgun sequence.
ACCESSION   DATADN010000407 DATADN010000000
VERSION     DATADN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504768"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VHH53_05340"
     CDS             <1..541
                     /locus_tag="VHH53_05340"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX2289614.1"
                     /translation="SGECDLNVIARRADAEIVAHGPNGRFVTAVLARLDTSTGKLSYL
                     VAGHPPPLLLRASKTVKALQYGRRTPLGVYGRDVEVVDEHLEPGDRLLLYTDGITEAR
                     DARGRPFGTGRLIDFAERSGAGGLPAPETLRRLAHDVLKYQGGLLQDDATLLMLDWRP
                     VNPDVAPLPVDVAPLPVTE"
     gene            614..925
                     /locus_tag="VHH53_05345"
     CDS             614..925
                     /locus_tag="VHH53_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289615.1"
                     /translation="MLADDAREIAAAIEERLNASACQGSKATVKTDQMSPKAVPAGAG
                     RPTFVRYFIQVDDGTRMATLSLGQAAALHDDINPDWDPDRLFEAIRTLDAPVETRAED
                     D"
     gene            985..>1535
                     /locus_tag="VHH53_05350"
     CDS             985..>1535
                     /locus_tag="VHH53_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2289616.1"
                     /translation="MRRATRDELRRVADETFGWSQLHDEQLQAMEQTMAGHDVLAVLP
                     TGAGKSAIYQVPALLLEGPTLVVSPLIALQQDQLEGIADTRAPQAVAVNSTQSGGEHQ
                     QAWDAIRRGEAEYAFLGPEQLAKDEVVDALADLRVSLFVVDEAHCVSSWGHDFRPDYL
                     RLGHVIQRLGHPRVVALTATAALP"
BASE COUNT          277 a          492 c          497 g          269 t
ORIGIN      
        1 cagcggcgag tgcgacctca atgtgattgc gcgccgcgcc gacgccgaga tcgtcgccca
       61 cggtccgaac ggccgtttcg tcactgcggt cctggcacgc ctggacacta gtaccggtaa
      121 gttgagctac ctcgtcgccg gacatccccc gcccctgctg ctgcgggcga gcaagactgt
      181 gaaagcactt cagtatggcc gtcggacccc cctgggcgtg tacggccgcg acgtcgaagt
      241 cgtcgacgaa catctggagc cgggtgaccg gttgctgctc tacaccgacg gcatcaccga
      301 agcccgggat gctcgtggcc gacctttcgg aaccggccgg ttgatcgact tcgccgaacg
      361 cagcggtgca ggtggtctac cggcaccgga gaccctgcgg cggctggccc acgacgttct
      421 caagtaccag ggaggcctgc tccaagatga cgccactttg ctcatgctcg actggcgacc
      481 agttaacccc gacgtcgcgc cgcttcctgt agacgtcgcg ccgcttcctg taactgaatg
      541 accctgtact tgtagaacaa acaccgggac tggtgtgccg cggtggagca actgttgcga
      601 aggagatagt ccaatgcttg cagacgatgc ccgggaaatc gccgccgcga tcgaggaacg
      661 tctcaacgcc agcgcctgcc aagggagcaa ggccacggtc aagaccgacc agatgagccc
      721 caaggcggtc cctgccggcg caggacgtcc gacctttgtc cgatacttca tacaggttga
      781 tgacggcact cggatggcta ccctcagcct tggccaagca gccgcgttgc acgacgatat
      841 caacccggat tgggatccgg atcggctctt cgaggcgatc cgcaccctgg atgcacccgt
      901 tgagacacga gctgaggatg attgacagcc ctgatcacgt agcggcgtgt ttccctgctg
      961 ccgctgtggg tatcgcccac aaccatgcga cgcgcgacgc gggatgagct acggcgggtg
     1021 gccgatgaga cctttggttg gtcgcagctg catgacgagc agctccaggc gatggagcaa
     1081 accatggccg gacacgatgt gctggccgtg ctgcctaccg gtgcgggtaa gtctgcgatc
     1141 taccaggtgc ccgcgttgct gctcgagggc ccgacgctgg tggtgtcccc gctgattgcg
     1201 ctgcagcaag accagctgga gggcatcgcg gacacccggg cgccacaagc ggttgcggtg
     1261 aactccacgc aaagcggtgg cgagcaccaa caggcgtggg acgcaattcg ccgaggcgag
     1321 gcggagtatg ccttcctcgg gcccgagcaa ttggccaagg atgaagtcgt cgacgcgttg
     1381 gcggatctga gagtatcgct gttcgtggtc gatgaggcgc attgcgtctc atcctggggg
     1441 cacgacttcc ggcccgatta ccttcgtctg ggccatgtga tccagcggct cggccacccc
     1501 agggtggtcg cgctgaccgc caccgccgcg ctgcc
//
LOCUS       DATADN010000408         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_652303, whole genome shotgun sequence.
ACCESSION   DATADN010000408 DATADN010000000
VERSION     DATADN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652303"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..98)
                     /locus_tag="VHH53_05355"
                     /pseudo
     CDS             complement(1..98)
                     /locus_tag="VHH53_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
     gene            68..454
                     /locus_tag="VHH53_05360"
                     /pseudo
     CDS             68..454
                     /locus_tag="VHH53_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            451..891
                     /locus_tag="VHH53_05365"
     CDS             451..891
                     /locus_tag="VHH53_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289617.1"
                     /translation="MTDLRGRSFSGLVAGLNAGQHLTVDAVVAFVDGELGAAARGRAA
                     QHLSACQSCTAEVGVQRLARSMMRSAQCPQVPAELLAALRDIPHTAELSGPPDGLAVA
                     ADGTVVEIADPSRVPTAPLGSGVPLGASQRWGTGPSVLGRWMGR"
     gene            1067..>1528
                     /locus_tag="VHH53_05370"
     CDS             1067..>1528
                     /locus_tag="VHH53_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX2289618.1"
                     /translation="MDPAAARVFGRPAGTLGAFAVQTGDTPATRISEDLSTAAAPSPD
                     ALAAAFSRPSGSVERLQRPPGRNGVHPEYGAAGEDHFWPQGAGRSPWRDPWAAAVLGP
                     PAVAEHHRDPGDPPQRAARLSLQEALFGRQLSHRAIAVLGAMVLLIGGLGGF"
BASE COUNT          221 a          509 c          543 g          255 t
ORIGIN      
        1 tccgggctga ggccgtcacc gaccaaccgg tcggtgtcct cgggcaggcc ttccatccgc
       61 agccgggaac gtcgccgcac catgtccagg aacaggttgg ctgcaccgga tcacgaccaa
      121 cctgttcctg gacatggtgc ggcggcgttc ccggctacgg atggaaggct tgcccgagga
      181 caccgaccgg ttggtcggtg acggcctcag cccggagcag gtctacgccg agacccacct
      241 cgatccggat ttgcagcagg ccctagacga tctccctccg gagttccggg cagccgtcgt
      301 actctgtgac gtcgagggcc tgtcatacga ggagatcggc gctacgctcg gggtgaagct
      361 cgggaccgtt cgcagccgga ttcaccgcgg tagactggca ctgcggacgg cgctcgaacg
      421 gcgtcgtgca ctcgctgacg aggaggctgg atgaccgacc tgcgcggccg atctttctct
      481 ggtctggtag ccggcctcaa cgcaggacaa cacctcaccg tggatgcggt ggtggccttt
      541 gtcgatggag agctcggtgc ggctgcccgt ggtcgggctg ctcaacatct cagcgcttgc
      601 cagtcctgca ccgcggaggt gggcgtgcag cgcctggccc gcagcatgat gcgttcggcg
      661 cagtgcccgc aggtgcccgc cgagttgctc gccgcgttgc gagacatccc gcacaccgct
      721 gaactgtccg gtcctccgga tgggctggcg gtggctgccg atggcaccgt cgtcgagatt
      781 gccgacccca gccgggtccc gaccgcaccg ctgggtagcg gcgtcccgct gggcgccagc
      841 cagcgttggg gtaccggacc gtcggtactg ggacgttgga tgggtcggta acagcgctac
      901 gcatacttca ccgggttgca ggcaggctcg gtggtcttga gcgtgccgag ttgaagcccg
      961 ggagaccatg agcccgccgc aggatcacca cccagacacg ccttttgcca tgcaagggcg
     1021 ttcggggccg ccgcggttcg tgccaaggcc gttggagcat cccgaggtgg atccagcggc
     1081 agcacgggta ttcggtcggc ccgccgggac tctcggagcc ttcgccgttc agaccggtga
     1141 cacgcccgct acccgcatat cggaagactt atcgaccgcc gctgcgccgt cccccgatgc
     1201 gctggcggca gcgttcagca ggccgtcggg atccgtcgaa cggttgcagc gcccgcccgg
     1261 ccgcaacggc gtgcaccctg agtatggggc cgcaggcgag gatcacttct ggccgcaggg
     1321 cgctggccgc agcccgtggc gtgacccgtg ggcagcggcg gtgctggggc cccccgcggt
     1381 tgccgaacac caccgggatc ctggcgaccc gccgcagcgg gcagcccggt tgagcctgca
     1441 ggaagcgctg ttcggccgtc aattgagtca ccgtgccatc gcggtgttgg gcgcgatggt
     1501 gctgttgatc gggggactgg gcgggttc
//
LOCUS       DATADN010000409         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1411642, whole genome shotgun sequence.
ACCESSION   DATADN010000409 DATADN010000000
VERSION     DATADN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411642"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..647)
                     /locus_tag="VHH53_05375"
     CDS             complement(90..647)
                     /locus_tag="VHH53_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2289619.1"
                     /translation="MRAALAGMDDAEGAQIDFWGRHLAGVLTTGGRLLVCGNGGSAAE
                     AQHLTGELVGRYLHDRRPLSAIALHADTSALTAIGNDYGGHETFARQVFAHGRPGDVL
                     IALSTSGISRNVVSAAKAAHEIGMTAWALTGPAPNTLAAVCDDAIAVEAPSMATVQEV
                     HLAVIHALCAVVDDALGVVPPTAPL"
     gene            complement(668..1918)
                     /locus_tag="VHH53_05380"
     CDS             complement(668..1918)
                     /locus_tag="VHH53_05380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289620.1"
                     /translation="MRIGLISEHASPLAAIGGVDSGGQNVHVAQLAQALGRRGHHVTV
                     YTRRDSSELPERVRMAPGVTVEHVRAGPAVPLPKDELLAHMAEFGEHLARRWVQQPPD
                     VVHAHFWMSGLAAQRGVRSIGSTGIPVVQTFHALGVVKRRHQGAADPSPPSRLSLEHD
                     LARDAALVIATCSDEVTELAALDVPAQRVAVVPCGVDLDRFCPHGPRASRDGRYRILS
                     LGRLVPRKGVETTIRALTGVPSAELIIAGGGDATTEAERARLAAIAQDCEVSDRVRLI
                     GHVPRKDVPALLRSADVVVSVPWYEPFGMVPLEAMACGVPVVASAVGGHLDTVVEGRT
                     GALVPPRDAGALAQRLRELLADPGRLAAIGSAAAARARSRYGWDRIAGDTEQLYRRVL
                     ADVPNFAANQTEEDPTATPAGGRS"
     gene            complement(1915..2865)
                     /locus_tag="VHH53_05385"
     CDS             complement(1915..2865)
                     /locus_tag="VHH53_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289621.1"
                     /translation="MRVLHWHAHGSWSTAFVQGAQTYLVPVTEDRGADGRGRARTYQW
                     PDNAIEVTPAALATEHVDVVVLQRPHELDLVEQWLGRRPGRDVPAVYVEHNAPRGDVP
                     NTRHPMADRSDIPVVHVTPFNELFWDCGRAPTVVIEHGIVDPGYRYTGDIPHAGVVIN
                     EPVRRGRVTGTDLLPRFAARVPLDVFGMVVSSLPPHDRIATFDDLPQARMHAELARRR
                     VYLHPIRWTSLGLSLLEAMHLGMPVVALATTEASQAVPPHAGVVSTRVDLLTDALSTF
                     IADPDLARRTGLAARAAARQRYSLDRFRTDWDRLLKEVTR"
     gene            complement(2862..>3155)
                     /locus_tag="VHH53_05390"
     CDS             complement(2862..>3155)
                     /locus_tag="VHH53_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX2289622.1"
                     /translation="TTTRAPSSTAASVVVVGNTGPAHLAAAVGTPVVSLFAPVVPAAR
                     WAPYGVPTVLLGDQDAPCRDTRARHCPVPGHPCLAGVTPLDVVEAVDALLVAA"
BASE COUNT          465 a         1156 c         1111 g          423 t
ORIGIN      
        1 gacacagccg gttcacggtg ccggtgatgt cactgtccag cagggtgtcg ccgagcacca
       61 ccagcctgga gccgctcact gatgcgccct catagcggcg ccgtgggcgg gacaacaccc
      121 agcgcatcgt ccaccacggc gcacaacgcg tggatcaccg cgagatggac ctcctgcacg
      181 gtggccatgc tgggcgcttc caccgcgatc gcgtcgtcgc agaccgccgc cagcgtgttg
      241 ggcgccggac cggtcagcgc ccacgcggtc atcccgatct cgtgcgccgc cttggccgcg
      301 gagaccacat tgcggctgat gccactggtg gacagggcga tcagcacgtc acccgggcgc
      361 ccgtgggcga agacctgccg ggcgaacgtc tcgtgcccgc cgtagtcgtt gccgatcgcg
      421 gtgagcgcgg aggtgtcggc gtgcagggcg atcgccgaca gcgggcgccg gtcgtgcagg
      481 taccggccga ccagctcgcc ggtgagatgc tgggcttctg cggcgctgcc gccattgccg
      541 cacaccagca gccgcccccc ggtagtcagc acacccgcta ggtgccgtcc ccagaagtcg
      601 atctgcgcgc cttcggcgtc gtccatcccg gccagggcgg cgcgcagcgc ggcgaagtgt
      661 ccccggctca cgaccggcct cccgccggcg tggcggtggg atcttcctcg gtttgattcg
      721 ctgcgaaatt tggcacgtcc gccagcactc ggcggtacag ctgctcggtg tcacccgcaa
      781 tccgatccca gccgtaacgg gaacgcgccc gggccgcggc ggccgagccg atcgcagcca
      841 gccgtcccgg atcggccagc agttcccgca accgttgcgc cagtgcgccc gcatcccgcg
      901 ggggcaccag cgcaccggtg cggccctcca ccacggtgtc caggtgcccg cccaccgccg
      961 aggcgaccac cggcacaccg caggccatcg cctccagcgg caccatgccg aacggttcgt
     1021 accacggcac gctgaccacc acgtcggctg agcgcagcaa cgcaggaaca tccttgcgcg
     1081 ggacatggcc gatcagccgg acccggtcgg atacctcaca gtcctgcgcg atcgcggcca
     1141 gccgggcgcg ttcggcttcg gtggtcgcgt cccccccgcc agcgatgatc agttccgcgc
     1201 tgggcacgcc ggtcagcgcg cggatcgtgg tctcgactcc cttgcgcggc accaaccgcc
     1261 cgagcgacag gatgcggtag cgaccgtcgc gcgacgcccg aggcccgtgc gggcagaacc
     1321 ggtcgaggtc gacaccgcag ggcaccacgg cgacccgctg cgccggcacg tccagggcgg
     1381 caagttcggt gacctcgtcc gagcaggtcg cgatcacgag tgcggcgtcg cgggccaggt
     1441 cgtgttccag tgacaaccgc gacggcgggc tggggtccgc cgcgccctgg tggcgccgct
     1501 tcaccacgcc caacgcgtgg aaggtctgca ccaccgggat cccggtgctc ccgatacttc
     1561 ggacgccgcg ctgcgcggcc agcccgctca tccagaagtg ggcgtgcacg acatccggtg
     1621 gctgctggac ccaccggcgc gccaggtgct cgccgaactc ggccatgtgg gcgagcagct
     1681 cgtccttggg caggggaacg gcgggcccag cgcggacgtg ctcgaccgtc acccccggtg
     1741 ccatccggac ccgctcgggc agctcagagc tgtcgcggcg ggtgtacacc gtcacgtgat
     1801 gaccgcgccg gcccagcgcc tgggccagct gcgcaacgtg cacgttctgc ccgcccgagt
     1861 ccaccccacc gatcgccgcg agcgggctgg catgctcgga gatcaagccg atcctcaccg
     1921 cgtcacctcc ttcagcaacc ggtcccagtc ggtgcggaac cggtccagcg aatacctctg
     1981 gcgggcggcg gctctggccg ccaggccggt gcggcgggcg agatcaggat cggcgatgaa
     2041 ggtgctcagc gcgtcggtga gcaggtcgac tcgggtggac accaccccgg cgtgcggtgg
     2101 caccgcctgc gatgcctcgg tggtagcgag cgcgaccacc ggcatgccca ggtgcatggc
     2161 ttcgagcagg gacaaaccca gcgatgtcca gcggatcggg tgcaggtaca cccggcggcg
     2221 ggcaagttcg gcgtgcatcc gcgcctgcgg cagatcgtcg aacgtcgcga tccggtcgtg
     2281 cggtggcaac gaggagacca ccatgccgaa gacgtccagc ggtacccggg cagcgaagcg
     2341 tggcagcagg tcggtgccgg tgacccgccc gcggcggacc ggctcgttga tcaccacccc
     2401 ggcgtgcggg atgtccccgg tgtagcggta gccggggtcc acgatgccgt gttcaatcac
     2461 cacggtcggg gcccggccgc agtcccagaa cagctcgttg aacggcgtga cgtgcaccac
     2521 gggaatgtcg gaccggtcgg ccatcgggtg ccgggtgttg ggcacgtcac cccggggggc
     2581 gttgtgctcg acgtacaccg cgggcacgtc gcgtcccggg cgccgaccca gccactgctc
     2641 gaccaggtcc agctcgtgcg ggcgttgcag caccaccacg tcgacgtgct cggtggccag
     2701 cgccgccggg gtcacttcga tcgcgttgtc cggccactgg taggtccgcg cgcgaccgcg
     2761 accgtcggca ccacggtctt cggtgaccgg caccaggtag gtctgggcgc cctgcacgaa
     2821 ggcggtgctc caggatccgt gggcatgcca gtgcagcacc ctcatgccgc caccagcagc
     2881 gcatcgaccg cctcgacgac gtcgagcggt gtcaccccgg ccaggcaggg atggccgggc
     2941 acggggcagt gccgggcccg ggtgtcgcgg cagggcgcat cctggtcacc gagcagcacg
     3001 gtaggcaccc cgtaaggggc ccagcgcgcc gcgggcacca cgggggcaaa cagcgacacc
     3061 accggtgtgc cgaccgcggc agccagatga gccggaccgg tgttgccgac aacgaccacg
     3121 ctggccgccg tgctcgatgg tgcccgcgtg gtcgt
//
LOCUS       DATADN010000410         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_358875, whole genome shotgun sequence.
ACCESSION   DATADN010000410 DATADN010000000
VERSION     DATADN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358875"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..639
                     /locus_tag="VHH53_05395"
     CDS             496..639
                     /locus_tag="VHH53_05395"
                     /inference="COORDINATES: protein motif:HMM:NF044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289623.1"
                     /translation="MARWTTHYRATDSDAVLVHPDRFIAWRSAEHSNPQELEAALRHV
                     LDR"
     gene            complement(720..>2185)
                     /locus_tag="VHH53_05400"
     CDS             complement(720..>2185)
                     /locus_tag="VHH53_05400"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX2289624.1"
                     /translation="IDALVLTHGHEDHIGAVPFLLRLRPDLPVVGSEFTLALVAEKCR
                     EHHQKPKLIQVTEGVRHGCGPFDCEFFAVNHSIPDALAVAIRTPAGLVLHTGDVKLDQ
                     LPLDGRLTDLAGFSRLGDEGVDLLLIDSTNAEVPGFVTPERDIGPVLDSVIGKATQRV
                     IVACFASHVHRVQQVLDVAAGHGRKVALVGRSMVRNMGVAVDLGLLQVPPGLLVDLDE
                     ALAMPEHRVLFVSTGSQGEPLSALSRMARGEHRQIRIRSGDTVVLASSLIPGNETAVF
                     RVVNGLTRLGAAVVHQGTAKVHVSGHAPAGELLFLYNAIRPSNVMPVHGEWRHLRANA
                     ALAVRTGVSPERVVIAEDGVVVDLVDGRAEITGRVEVGQVYVDGLSVGDVGESTLSDR
                     LVLGEGGFIAITVVVASATGRAVAPPTISGRGFSDDPKALDDVVSLVEMELARTEAEG
                     ITDTHRIAQAVRRVVGRWVADTYRRRPMIVPTVLPVG"
BASE COUNT          396 a          800 c          666 g          323 t
ORIGIN      
        1 acggacaaca tcccctccat gatgcgtacc gcgttgggcc cggtcggaac cctggtcgcg
       61 atggccagat gggcgctgcc ggggtgatcg gtcatcaccc ggaccgtcga gtcgtagagc
      121 agcaatacgc caatctcgcc agcggatggc gccccacctc gccgacggca gcgaggcagc
      181 acaaatcgac tctgccaccc tgcgcctcgg ctaccgctac cccgaccggc gccatcgtcg
      241 gcgagcctgg cgatgacgga tctctgatcg aggacccaag cgcgccccca ccggccgccc
      301 aggttcccgc gcaccgcacg ctcccctgct tcgtgacaat gtgccgatct ccacgattga
      361 tctctttgga gattcctttg tgctcctcac aggcagccgt ggccagcact ggcgtcaggc
      421 cggtggcgag gtttcgcacc ggctcgacat ccggctggac acctaccaga tcggcaccga
      481 ggacgtggtg gaccgatggc acgctggacc acccactacc gcgccaccga ctccgacgcg
      541 gtcctcgtcc accctgacag gttcatcgcc tggcggtcag ccgagcacag caatccacaa
      601 gagttggagg ccgcgctgcg gcacgtcctg gaccggtaag ccgtctactc gcgacgggtt
      661 ggtagtcgaa atatcagctg cgaagggggt tggcggtact tgaagggcag ccgaattagt
      721 cagccgacgg gcagcacggt aggcacgatc atcgggcggc gccggtaggt gtcggcaacc
      781 caccgaccca ccacccgccg cactgcctgg gcgatccgat gggtgtcagt gattccctcc
      841 gcttcggtgc gggccagctc catttccacc agcgacacga cgtcgtcgag ggccttggga
      901 tcatcggaga atccgcggcc cgagatcgtg ggaggagcca ccgcccggcc ggtggctgac
      961 gccaccacca cagtgatcgc gatgaaccca ccctcgccga ggaccagccg atcggacagc
     1021 gtcgactccc ccacgtcgcc gaccgagagc ccgtccacat aaacctgccc cacctccacc
     1081 cgtccggtga tctccgcgcg gccgtccacc agatccacga ccacgccgtc ctcggcgatc
     1141 accacccgtt ccggcgacac cccggtacgg accgcgagtg cggcgttggc ccgcagatgc
     1201 cgccactcgc cgtgcactgg catgacgttg gaaggccgga tcgcgttgta gaggaacagc
     1261 aattccccag ccggtgcgtg cccggaaacg tgcacttttg ccgtgccctg gtgcacgacc
     1321 gcggcgccca accgggtcag cccgttgact acccggaaca ccgccgtctc gttgcccggg
     1381 atcagcgaac tggccagcac aacggtgtcc ccggaacgga tccggatctg ccggtgctcc
     1441 ccgcgagcca tccgggacag cgccgacaac ggctcaccct gggagccggt ggagacgaac
     1501 agcacccgat gctcgggcat cgccagcgcc tcgtcgagat ccaccagcag acccggcggg
     1561 acctgcagca ggcccagatc aaccgcgacg cccatgttgc gcaccatcga tctgcccacc
     1621 agcgccacct tgcgtccatg cccggcggcg acgtccagca cctgctgcac tcggtgcaca
     1681 tggctggcga agcaggccac gatgacgcgc tgagtggcct tcccgatcac cgagtccagc
     1741 acaggaccga tatcacgctc cggcgtgacg aagcccggca cctcggcgtt ggtggagtcg
     1801 atcagtagca gatccacccc ctcgtcgccg agccgggaaa accccgcaag atcggtcagc
     1861 cgcccatcca gcggtagctg gtcgagcttg acgtcaccgg tgtgcagtac cagacccgcc
     1921 ggcgtgcgga ttgcgaccgc caacgcgtcc gggatggaat ggttcaccgc gaagaactcg
     1981 cagtcgaacg gcccgcagcc gtgccgtaca ccctcggtga cctgaatcag cttcggcttc
     2041 tggtgatgct cccggcactt ctcggcgacc agcgcaaggg tgaactccga tcccaccacc
     2101 ggcagatcgg gacgtaaccg cagcaggaac ggcacggccc cgatgtggtc ctcgtgccca
     2161 tgggtgagca ccagcgcatc gatgt
//
LOCUS       DATADN010000411         2724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_359124, whole genome shotgun sequence.
ACCESSION   DATADN010000411 DATADN010000000
VERSION     DATADN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359124"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1470
                     /locus_tag="VHH53_05405"
     CDS             25..1470
                     /locus_tag="VHH53_05405"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2289625.1"
                     /translation="MADRGNTMPSFDYDLAIIGAGSGGLTAARLGRFFAKRVALIDKD
                     RLGGDCLHYGCVPSKALIKASRVTHEAATAQRWGLSAQPVNNGLAAVNARVQRAIADI
                     GKLDDTVALSELGVDVLLGGATFRNGHTLRVGGGDIEIRDITAKFILIASGSRPAVPA
                     IEGLTEVGYLTNEDVFDLSDLPARLAVIGGGPIGVELAQAFHRLGSTVTVLQRGLHLI
                     PRDDAEMIDVLEHQFAEEGIDVRLDTRLHAVRRDGGDTVLTHDGGELRVDEILCAVGR
                     TPNVESLGLDVAGVVTNRRGIAVNELLQTNQPHIYAVGDVTGGPAFTHYAGHQAAHAV
                     RNIFVPIKAKFAPGHLPWVTFTDPEIAHVGRTEEQARAAGDTFQVVRFPYSHNERAVT
                     DAEPVGLMKFLIDGKRRLIGAHIAGQCAGEMINELTLAMNNDLTVDAIINSIHAYPTY
                     SFAIPIALYDYALTENPSTAARVGRFLSRLT"
     gene            1616..2599
                     /locus_tag="VHH53_05410"
     CDS             1616..2599
                     /locus_tag="VHH53_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289626.1"
                     /translation="MPQATRALRPDRRSRWTHSTSSNGVALSRADEAVGLAAADLELL
                     AMAAFLVGRDDEGARVMERAHHLYLEASDPVGAARCAIWTGFGRFDMGELAQAIGWYS
                     RAGKLLNRADRDCAERGYLLVPDVLQSLDSDTEAAYRTAATIAEIGERCTDADLVTFA
                     LHAQGRVRIRQGRVTEGMALLDEAMVAVVVGELASPLFTGLIYCSVIDACREVYDLGR
                     AREWTAALTRWCDAQPGLVNFTGQCLVHRAEIMQLRGEWRDSLEEARRAGERFSRGQR
                     GGRRGLPLPAGRGAPAAGGVCRRRGGIPRRQRLGVAATARSVPVAAGPGPY"
BASE COUNT          446 a          919 c          928 g          431 t
ORIGIN      
        1 cgctgcccgt aacgacgacc aaccgtggct gatcggggaa acacgatgcc gtccttcgac
       61 tacgacctgg cgattatcgg cgccggatcg ggtgggctga ccgctgcccg gctcggccgg
      121 ttcttcgcca agcgggtcgc gttgatcgac aaagatcggc tgggtgggga ctgcctgcac
      181 tacggctgcg tgccgagcaa ggcgttgatc aaggcgtccc gggtgaccca cgaagccgcc
      241 actgcgcagc ggtggggact gtccgcgcag ccggtgaaca acggcctcgc cgcagtcaac
      301 gcccgggtgc agcgagccat cgccgacatc ggcaagctgg acgacaccgt ggcgttgtcc
      361 gagctcggag tggatgtcct gctcggcggc gccaccttcc gcaacggaca cacgttgcgc
      421 gtgggtggcg gagacatcga aatccgggat atcaccgcca agttcatcct gatcgcctcg
      481 ggcagccgac cggcggtacc tgccatcgag gggctgaccg aggtcggtta cctcaccaat
      541 gaagacgtct tcgacctcag cgacctcccg gctcggctcg cggtgatcgg tggcggtccg
      601 atcggcgtgg agctcgcgca ggcatttcac cggctcggct ccacggtcac ggtgctgcag
      661 cgcgggctcc atctgatccc ccgcgacgac gccgagatga tcgacgtgct ggagcaccag
      721 ttcgccgagg agggcatcga cgtacggctg gatacccggc tgcacgcagt gcgacgcgac
      781 ggcggggaca cggtgctcac tcacgacggc ggcgagctgc gggtggacga gatcctgtgc
      841 gcagtcgggc ggacgcccaa tgtggaatcg ctcggattgg acgtcgccgg agtcgtcacc
      901 aaccggcgag ggatcgccgt caacgagctg ctccagacca accagccgca tatctacgct
      961 gtcggcgacg tgaccggcgg gccggcgttc acccactacg ccggtcacca ggcagcgcat
     1021 gcggtacgca acatcttcgt cccgatcaag gcgaagtttg cccctgggca cctaccgtgg
     1081 gtgaccttca ccgacccgga gatcgcgcac gtcggtcgca ctgaagagca ggcgcgtgcg
     1141 gcaggtgaca ccttccaggt cgtccgcttc ccctacagcc acaacgagcg cgccgtgacc
     1201 gatgccgaac ccgttggtct gatgaagttc ctcatcgacg gcaaacggcg gctcatcgga
     1261 gcccacatcg ccgggcagtg cgccggcgag atgatcaatg agctgaccct ggcgatgaac
     1321 aacgacctca ccgtcgacgc gatcatcaac tcgatacacg cttacccgac ctactcgttc
     1381 gccatcccga tcgcgctcta cgactacgca ctgaccgaga atcccagcac cgcggccagg
     1441 gtcggccggt tcctcagccg cctcacctag tgtcagcgac gacctccacg ctgcccactg
     1501 ggtgcgccct cgccgacgac gatgaggtga tcgcgaagtt gccgaacacg gcgtcgacgt
     1561 tgaagcctga ccgtgcccca ggtacacctc gtggatctgc gaccgcacgc tgtcggtgcc
     1621 gcaagcaacg agagctctcc ggccggatcg gagatctagg tggactcact cgaccagctc
     1681 gaacggggtc gcgctgtcgc gggccgacga ggcagtcggt ctggcggccg ccgatctcga
     1741 gttgctggcg atggcggcgt ttctcgtcgg ccgggacgac gaaggcgccc gcgtgatgga
     1801 gcgggctcat cacctttatt tggaggcctc cgacccggtc ggtgctgcgc ggtgtgccat
     1861 ttggaccggt ttcggtcgct tcgacatggg cgagctcgcc caggcgatcg ggtggtacag
     1921 ccgggcaggc aagctcctca accgcgccga tcgggactgc gccgagcgcg gctaccttct
     1981 cgtgcctgat gttctccagt ccctcgacag tgataccgag gcggcgtatc gcaccgcggc
     2041 caccatcgcg gagatcggtg agcggtgcac cgacgccgac ttggttacct tcgcgctgca
     2101 cgcgcagggc cgggtccgga tcaggcaggg acgcgtcacg gagggcatgg cgctgctgga
     2161 tgaggccatg gtcgcggtgg tcgtgggaga gctggcttcg ccgctgttca ccggactgat
     2221 ctactgcagc gtgatcgacg cctgccggga ggtctacgac ctcggccgcg cccgcgaatg
     2281 gacggccgcg ctgacccggt ggtgtgacgc ccaacccggc ctggtcaact tcaccggcca
     2341 gtgcctggtg caccgcgctg agatcatgca gctgcgcggc gaatggcggg actccctgga
     2401 ggaggcgcgc cgcgcaggtg aacgtttctc ccgaggccag cgaggcggcc gccgcggcct
     2461 gccactacca gcaggcagag gtgctccggc tgctggggga gtttgccggc gccgaggagg
     2521 cataccgcgc cgccaacgcc tgggggtggc agccacagcc cggtctgtcc ctgttgcggc
     2581 tggcccaggg ccgtactgat gccgcagccg ccgcgatctg ccgggtgctg ggcgggacca
     2641 cgcaacgcct ggaacgtgcc cggttgctgc cggcgtacgt ggaatcatgc tggccaccgg
     2701 cgacaacgcc agcgcgcgca gggc
//
LOCUS       DATADN010000412         1897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_652867, whole genome shotgun sequence.
ACCESSION   DATADN010000412 DATADN010000000
VERSION     DATADN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652867"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VHH53_05415"
     CDS             complement(<1..142)
                     /locus_tag="VHH53_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX2289627.1"
                     /translation="MHQLRLPIPTRVVVLVSGTGTLLQALLDAAGPDYPALVVAVGAD
                     RAG"
     gene            complement(189..899)
                     /locus_tag="VHH53_05420"
     CDS             complement(189..899)
                     /locus_tag="VHH53_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2289628.1"
                     /translation="MTLFRYTAVLTARAGRPRNTSIDVAVLDATDAVLGDSGYSRFTL
                     EEVARRAGTTKPAIRRRWPKRQHLVLAVLARRLGAAQVPDTGCTLCDLTDGINVFVAA
                     FQRMPPDVLGPLLAECAGESELRADFMATLFDPPRAAVAQTLDRALARGDLRADLDRE
                     LTLDLLGSLIHYRTLFGHAPTSDAAIERAVETILGGIAADYPALLEHSRRRAGDPQTH
                     HLHDGSLNQADPSSMGAR"
     gene            988..>1897
                     /locus_tag="VHH53_05425"
     CDS             988..>1897
                     /locus_tag="VHH53_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2289629.1"
                     /translation="METQMSSNIAPDTGPKAGRREWIGLAVLALPTMLIGLDITVLHL
                     AVPQLSVDLQPSSSQLLWIVDIYGFLIAGFLLTMGTLGDRIGRRRLLLVGATAFALAS
                     GLAAFSSTPEMLIATRALLGIAGATLMPSTLSLIRNMFLDAQQRTVAISAWMTSLMAG
                     TAIGPLVGGVLLAHFWWGSVFLLSIPVMVLLLAAAPVLLPEYRNPASGRVDPSSVVLS
                     LAAILPVVYGLKESATHGLGWLPALSIVVGLTTGALFISRQRTLADPMLDLQLFGDRA
                     FSASVTTHLLSLFLVAGIQYLIAQHLQ"
BASE COUNT          290 a          622 c          664 g          321 t
ORIGIN      
        1 ccccggctcg gtccgcgccg acggcgacaa ctaaagcggg gtagtccggc ccggcggcgt
       61 ccagcaacgc ctgcagcagc gtcccggtgc cggataccag gacaacgacc cgggtcggaa
      121 tcggtaggcg gagctgatgc acgagcagcg caggctcctg gcggcagggc ggatggcgag
      181 cactcagact agcgtgcccc catggagctc ggatcggcct ggttgaggga tccgtcgtgg
      241 aggtggtggg tctgcgggtc accggcccgg cggcggctgt gttcgagcag cgcgggatag
      301 tcggcagcga tgcctcccag gatggtttcg acggctcgct cgatcgctgc gtcgctagtg
      361 ggtgcgtggc cgaacaacgt gcggtagtgg ataagtgatc cgagcaggtc gagcgtgagc
      421 tcacggtcga ggtccgcacg caagtcccct cgggcgaggg cgcgatcgag tgtttgcgcg
      481 acggcggcgc gcggtgggtc gaacagcgtg gccatgaagt ccgcgcggag ctcggactcg
      541 cccgcgcact cagcaaggag ggggccgagc acgtccgggg gcatgcgttg gaaagctgcc
      601 acgaagacat tgatgccgtc ggtgaggtcg cacaatgtgc agccggtgtc cggcacttgc
      661 gccgcaccca ggcgccgggc gagcaccgcg aggacgaggt gctggcgctt gggccagcgg
      721 cggcggattg caggcttggt ggtgccggcg cggcgggcga cctcttcgag ggtgaaccgg
      781 ctgtagcccg agtcgccgag tacagcatcc gtggcgtcga gcacggcgac gtcgatggac
      841 gtgttccgcg gccgaccagc gcgcgccgtc agcacggcgg tgtagcgaaa gagtgtcaca
      901 gcactatgct acattccgat tcggaacgaa acgaaatagg gtgcgacttc ggcgcgctca
      961 gcgcagcgac cgcccgacca ggcaccgatg gagacacaga tgtcgtcgaa catcgcgccc
     1021 gacaccggcc cgaaggctgg ccgccgggaa tggatcggcc tcgccgtgct ggccctaccc
     1081 acgatgctca tcggtcttga catcaccgtg ctgcacctgg cggtgccgca gctgagcgtc
     1141 gatctccaac ccagcagctc ccagctgctg tggatcgtgg acatctacgg gttcctgatc
     1201 gccgggttcc tactgacgat gggaacgctg ggcgaccgaa tcggccgccg acggctgctg
     1261 cttgtcggcg ccaccgcctt cgccctcgct tcggggctcg ccgcgttttc ctcgacccct
     1321 gagatgctga tcgcgacccg cgcgctgctg ggcatcgcgg gggcgacgct gatgccatcc
     1381 accctgtcgt tgatccgcaa tatgttcctc gacgcgcagc agcgcacggt cgcgatctcg
     1441 gcgtggatga caagcctgat ggcaggcacc gcgatcggcc cgctggtcgg gggggtgctt
     1501 ttggcgcact tctggtgggg ctcggtgttc ctgctgagca tcccggtcat ggtgctgttg
     1561 ctcgcggcgg caccggtact gctgcccgag taccgaaacc ccgcatcggg tcgggtggac
     1621 ccgagcagcg tcgtcttgtc cctcgctgcc atcctgccgg tggtctacgg cctcaaggaa
     1681 tccgccacac acggcctcgg gtggctaccc gcgctgtcca tcgtggtcgg cctcaccacc
     1741 ggcgcgctct tcatcagccg gcaacgcacg cttgccgatc cgatgctcga cctgcaactg
     1801 ttcggcgacc gcgctttcag cgcgtcagtg accacgcact tgttgtccct gttcctcgtg
     1861 gccgggatcc aatacctcat cgcgcagcat ctgcagc
//
LOCUS       DATADN010000413         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1412058, whole genome shotgun sequence.
ACCESSION   DATADN010000413 DATADN010000000
VERSION     DATADN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412058"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..>1721)
                     /locus_tag="VHH53_05430"
     CDS             complement(108..>1721)
                     /locus_tag="VHH53_05430"
                     /inference="COORDINATES: protein motif:HMM:NF026246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2289630.1"
                     /translation="PTKWAVQFGAFAGLAGALGAVTTFAVARVGLHSRRNLALYVTAL
                     LFVLAWATSGINGWFYVGNYGVPWFDKQPVIAGIPVLGIFLVLAILTGLLAGWLHFRM
                     DYTGHTEVADTRRNRVLASTPLLVVAVIMVVLEVGSMAKGAVQRYPVYTTAKANASAL
                     VSGLSATSCAMADDVLAEPDPNAGLLQPVPGQRFGEYGPLGGEDPVGFTPNGVSDTLE
                     PPEPVTANPGTVNSDGSPNEPNIGIGFAAGTGGGYGPVGVNGSNVFLPFGLDPKRTPV
                     MGSYDENTVAAKVTSAWYQLPFNAGERTPERPLVTIAAAGAIWFFEEDGSFNYGQSLK
                     LQWGVHRPDGSYAPLAEVQPIDVGPGPQKAWRNLRFPLGWAPPEANVARIVADDPNLS
                     NDQWFAFTPPRVPVLETAQELLGSQTPVLMDIATGANFPCQRPFSEHLGVAELPEYRI
                     LPNFKQVVVSSNQWQSAQDGGPFLFIQALLRTSTVPTYLRDDWYRDWGSIERYDRVVP
                     ADDAPNAVIEQGSVRVFGWTRNGPIRALP"
BASE COUNT          294 a          570 c          586 g          271 t
ORIGIN      
        1 cgtggcggtg gtctgcacga ccggcagcag cggcgtcgcg accgacagca agaagccgac
       61 caggccggcg atcgccgcca cccaacgggc gaccttcacg tcgtctctca tggcagcgcc
      121 ctgatcggtc cgttgcgggt ccagccgaac acccgcaccg agccctgctc gatgacggcg
      181 ttcggtgcgt catcggcggg caccacgcgg tcatagcgct cgatcgatcc ccagtcgcga
      241 taccagtcgt cgcgcagata ggtcggcacg gtcgaggtgc gcagcagtgc ctgaatgaac
      301 aggaacgggc cgccgtcttg cgccgactgc cactgattcg acgacaccac aacctgtttg
      361 aaattcggga ggatgcggta ttcgggcagt tccgccacgc cgaggtgttc ggagaacggc
      421 cgctggcacg ggaaattggc tccggtggcg atgtccatca gcaccggcgt ctgcgaaccg
      481 agcagctcct gggcggtctc gagcaccggc acccgcggcg gggtgaacgc gaaccactgg
      541 tcgttgctca ggttcgggtc gtcggcgacg atgcgcgcca cattggcctc cggcggcgcc
      601 caccccaacg ggaatcgcaa gttgcgccaa gctttttgcg gtcccgggcc gacatcgatc
      661 ggctgcactt cggccagcgg tgcgtagctg ccgtcgggac ggtgcacacc ccactgcagt
      721 ttgagcgact gcccgtagtt gaacgagccg tcctcctcga agaaccagat cgcaccggcg
      781 gcagcaatgg tcaccagcgg ccgctcgggg gtgcgctctc ctgcattgaa aggcagttgg
      841 taccacgccg aggtgacctt ggcggcgacg gtgttctcgt cgtagctgcc catcaccggg
      901 gtgcgtttgg ggtcaagacc gaacggcagg aacacatttg atccgttgac gccgacgggg
      961 ccgtacccgc cgccggtgcc tgcggcgaac ccgatgccga tgttcggttc attcggggag
     1021 ccgtcggagt tcacggtccc ggggttggcg gtgacgggct cgggcggctc gagggtgtca
     1081 ctgacgccgt tgggtgtgaa gccgaccggg tcctcgccgc cgagcggacc gtactccccg
     1141 aagcgctgac ctggaaccgg ttgcaacaga ccggcattgg ggtcgggttc ggcgagcaca
     1201 tcgtcggcca tcgcgcagct cgttgcggac aggccggaca ccagcgccga ggcgttggct
     1261 ttcgcggtcg tgtagacggg gtatcgctgc acggcgccct tggccatcga cccgacctcg
     1321 aggacgacca tgatgaccgc caccaccaac agcggggtgg aggcgagcac tcggttgcgg
     1381 cgggtgtcgg cgacctcggt gtggccggtg tagtccatcc ggaagtgcag ccagccggcc
     1441 agcaatccgg tcaggatcgc cagcaccagg aagatgccga gtaccgggat accggcgatc
     1501 accggctgct tgtcgaacca cggcacgccg tagttgccga cgtagaacca gccgttgata
     1561 cctgatgtcg cccaagccaa cacgaacaac agcgcggtca catacagcgc gaggttgcgt
     1621 cggctgtgca gcccaaccct ggcgacagcg aaggtcgtca ccgcgcccag tgcgcccgcc
     1681 aggcccgcga acgcgccgaa ctgcacggcc cacttcgtcg g
//
LOCUS       DATADN010000414         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_961839, whole genome shotgun sequence.
ACCESSION   DATADN010000414 DATADN010000000
VERSION     DATADN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961839"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VHH53_05435"
     CDS             <1..972
                     /locus_tag="VHH53_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEX2289631.1"
                     /translation="GDVYFAVASFDDYGALSGRRLDEVCQGESAASGKRDPRDFALWK
                     AAKPGEPCWPTPWGPGRPGWHLECSAMATTYLGAEFDIHGGGLDLVFPHHENERAQSR
                     AAGDGFARYWLHIGWVTLSGEKMSKSLGNTLSVPATLRRVRAVELRYYLIGAHYRSAI
                     EYSDGALDEAVAAYRRIESFVHRIRERCGVPEPGTWCADFAVAMDDDLSTPAALAAIH
                     TTVHEGNAALDAGNHTAAMAATGSVRAMAGVLGLDPLDDQWRSTSEVDATSGALAVLV
                     DRLLAERAEARARRDYTTADAVRDQLLAAGITVEDTPDGPVWTVKES"
     gene            976..>1695
                     /locus_tag="VHH53_05440"
     CDS             976..>1695
                     /locus_tag="VHH53_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2289632.1"
                     /translation="MAGNSQRRGAMRKPGTKKGAVAGSGGQKSKGLRGRGATPPAELR
                     TGHPAQRPRRPAQGQSAQGQSAQRQPAQRGAGAGPANQAARVRRRTGTEPGETVLGRN
                     PVLECLRAGVPATALHVAIGVDTDDRISESVRLAADAGLSILEVPRADLDRLAGGALH
                     QGVALQVQPYEYARPEDVLAAALDSGTPPLLVALDGVTDPRNLGAVVRSVAAFGGHGV
                     LVPERRAAGMTASAWKTSAGAA"
BASE COUNT          250 a          573 c          624 g          248 t
ORIGIN      
        1 ggtgatgtct acttcgcggt agccagcttc gacgactacg gggcgctgtc cggacgacgt
       61 ctcgatgagg tttgccaggg tgaatccgcg gcatcgggca agcgagaccc ccgggacttc
      121 gcactgtgga aggcagccaa gcctggagaa ccgtgctggc cgacaccgtg ggggccgggc
      181 cgtcctgggt ggcacctgga gtgctccgcc atggccacca cgtacctcgg cgcggagttc
      241 gatatccacg gtggtggcct ggacctggtc tttccgcatc atgagaacga gcgggcgcag
      301 tctcgggccg ctggggacgg gttcgcccgg tactggctgc acatcggctg ggtgacgctg
      361 tccggtgaga agatgtccaa atcgctgggc aacacgctgt ccgtcccggc caccctgcgc
      421 cgggtccgtg ccgtggagtt gcggtattac ctgatcggcg cacattaccg gtctgccatc
      481 gagtactccg acggcgcgct ggatgaagcg gtggccgcct accggcggat cgagtcgttc
      541 gtgcaccgga tccgggagcg gtgcggggta cccgagcctg gcacctggtg tgcggacttc
      601 gccgtggcga tggacgacga cctcagcacc ccggccgcgc tggccgcgat ccataccacc
      661 gtgcacgagg gcaacgccgc gctggacgcg gggaaccaca ccgcggcgat ggcggccacc
      721 ggttcggtac gggcgatggc cggagtgctc ggcctggacc cgctggacga tcagtggcgc
      781 tcgacgtcgg aagtggatgc cacgtcgggg gccctggcgg tcctggtgga ccggttgctc
      841 gccgagcgcg ccgaggctcg ggcccggcgt gactacacta cggccgacgc ggtgcgcgat
      901 cagctgctcg ctgccgggat caccgtcgag gacactcccg acggcccggt gtggaccgtc
      961 aaggagagct gagtcgtggc cggtaattcc cagcgcaggg gagcgatgcg caagccgggc
     1021 accaagaagg gcgccgtcgc gggctccggt gggcagaagt ccaaggggtt gcgtggccgc
     1081 ggcgcgacgc cacccgccga actgcgaact ggccatccgg cccaacgtcc acgccgccct
     1141 gctcaaggcc aatcagccca aggccaatca gcccagcgtc aacccgctca gcggggggct
     1201 ggggctggtc ctgccaacca ggccgcccga gtccgccgtc gaaccggcac cgagccgggc
     1261 gagaccgtgc tcggtcgcaa cccggtattg gaatgcctgc gcgccggcgt gcccgccacc
     1321 gcactgcacg tcgcgatcgg agtggacacc gacgaccgga tcagcgagtc cgtacggttg
     1381 gccgccgacg ccggtctgtc cattctcgag gtacccaggg ccgatctcga ccggctcgcc
     1441 gggggtgcgc tgcaccaagg agtggcgcta caggtccagc cgtacgagta cgcccgtcct
     1501 gaggatgtgc tggccgcagc gttggactca gggacgccgc cgctgctcgt cgcgctggac
     1561 ggcgtgacgg acccgcgtaa cctcggggcc gtcgtccgct ccgtcgccgc gttcggcggg
     1621 cacggcgtcc tcgtgccgga gcggcgggcc gcggggatga cggcgtcggc gtggaagacg
     1681 tcagccggcg ccgcg
//
LOCUS       DATADN010000415         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1412086, whole genome shotgun sequence.
ACCESSION   DATADN010000415 DATADN010000000
VERSION     DATADN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412086"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /gene="trpS"
                     /locus_tag="VHH53_05445"
     CDS             <1..1247
                     /gene="trpS"
                     /locus_tag="VHH53_05445"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2289633.1"
                     /translation="HALDTVTGQELDRPIVRGADAGVMLDGMTDPARGSGRAAPIVGY
                     GHRAIMGRAVHFGPSIGPESVRYTRRPMSQLAVDPATDPEATRERVFSGIQPSGASHL
                     GNYLGAQVNYVAMQDQYDAIYGIVDLHAMTTVHDGTQLRELTRNMVLDLLAVGLDPDR
                     CAIIRQSDFPEHSELMWIFDTVVPVSWLERNPTYKEARAEGKENSNGLLTYPVLQAAD
                     IVIYKASRVPVGKDQAAHLELSREIVRAFNRRYGEIFPEPQAVFTDAPVVLGIDGQRK
                     MSKSAGNTIPIFAEPDEIHRLVMSMVTDPQRIKRSDPGRPEVCNVCGNLHRFFSPDYL
                     QIQEGERTARTGCVETKQLLAERIIEFFRPMREKRRQLAAEPAYVEEVLAAGAAKVRP
                     IIDATMAEVRAVVGIGRPSGGA"
     gene            1251..>1900
                     /locus_tag="VHH53_05450"
     CDS             1251..>1900
                     /locus_tag="VHH53_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2289634.1"
                     /translation="MRRAPVGALSTRERRWLLAFLVLGSAYFGLLLAQMAVSVMGGFS
                     QIILILFLAWLLAFVISPVVRFLDERLPLPRALAVVIAYLAALLLLGFVLFSVGASIT
                     REVTQVTTEFPQTAVQIEDRLAGYQAALGLERINIDLVELFQSAQRQVGVIAGDIFDQ
                     AEVVAGATLATLGSLVLILILSLYMLLDSERIWGKINRAVPRRYSDEVGLFERSVSR"
BASE COUNT          295 a          660 c          631 g          314 t
ORIGIN      
        1 gccacgccct cgacaccgtc accggccagg agctcgatcg cccgatcgtt cgtggagccg
       61 atgctggcgt gatgctcgat ggcatgaccg atccggcgcg tgggagcggc cgcgcggctc
      121 caatcgtggg ctacggtcat cgggccatta tgggcagggc ggtccacttc ggcccctcca
      181 tcgggcccga gagcgtgcgt tacactcgcc gtccgatgag ccagctggcc gtggaccccg
      241 cgacggatcc ggaggcaacc cgggaacgcg tcttctccgg catccagccg tccggcgcgt
      301 cgcacctggg caactacctc ggcgcgcagg tgaactacgt ggcgatgcag gaccagtacg
      361 acgccatcta cggcatcgtg gacctgcacg ccatgaccac cgtgcacgat ggaacccagc
      421 tgcgcgagct gactcgcaac atggtgctcg acctgctggc cgtgggcctc gacccggatc
      481 gctgcgcgat cattcgccag tcggacttcc cggagcactc cgagctgatg tggatcttcg
      541 acacggtggt cccggtcagc tggctggagc gcaaccccac ctacaaggag gcgcgcgccg
      601 agggaaagga gaacagcaac ggcctgctca cttacccggt gctgcaagcc gccgacatcg
      661 tcatctacaa ggcctcgcgc gtgccggtcg gcaaggacca ggcggcgcac ctggagctct
      721 cgcgcgagat cgtgcgcgcc ttcaaccgcc gctacggcga gatcttcccc gagccgcagg
      781 cagtcttcac cgatgccccg gttgtgctcg gcatcgacgg ccagcgcaag atgagcaaga
      841 gcgctggcaa caccattccg atcttcgccg agcccgatga gatccatcgg ctggttatga
      901 gcatggtcac cgacccgcag cgcatcaagc gcagcgaccc gggacgaccc gaggtctgca
      961 acgtgtgcgg caacctgcac cgcttcttca gcccggacta cctgcagatc caggagggcg
     1021 agcgcaccgc gcgcaccggc tgcgtcgaga ccaagcagct gctggccgag cgcatcatcg
     1081 agttcttccg cccgatgcgc gagaaacgtc gccagctggc tgccgagccg gcgtacgtcg
     1141 aggaggtcct cgccgcgggt gccgccaagg tgcgccccat aatcgacgcc accatggccg
     1201 aggtccgcgc agtggtcggc atcggtcgtc cgtccggggg agcctgaccg gtgcgccgcg
     1261 cgccggtggg agccctgtcc accagggagc gccggtggct gctcgccttt ctcgtgctcg
     1321 gctccgccta cttcgggctg ctgctggcgc agatggccgt ctccgtcatg ggcggcttca
     1381 gccagatcat cctgatcctg ttcctggcct ggctgctggc cttcgtgatc tcgccggtag
     1441 tgcgcttcct ggacgagcgg ctgccgctgc cacgggcgct ggcggtggtg atcgcctacc
     1501 tggccgcgct tctgctgctc ggcttcgtgc tcttctcggt cggagcctcg atcacgcgcg
     1561 aggtgacgca ggtcacgacg gagttcccgc agaccgcggt gcagatcgag gaccgcctgg
     1621 ccggatacca ggccgcgttg ggcctggagc ggatcaacat cgacctggtg gagctgttcc
     1681 agtcggcaca gcgccaggtg ggcgtgatcg ccggcgacat cttcgaccag gccgaggtgg
     1741 tggcgggggc cacgctagcc accctcgggt cgctggtgct gatcctgatc ctttcgctgt
     1801 acatgctgct cgactccgag cgcatctggg gcaagatcaa ccgagccgtc ccacgccgct
     1861 acagcgacga ggtcggcctc ttcgaacgga gcgtgtcgcg
//
LOCUS       DATADN010000416         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_359432, whole genome shotgun sequence.
ACCESSION   DATADN010000416 DATADN010000000
VERSION     DATADN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359432"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VHH53_05455"
     CDS             complement(<1..740)
                     /locus_tag="VHH53_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX2289635.1"
                     /translation="MTGARSVKALVGWKLGLSDATAKTVAAIADRFDELPRCTAEMRQ
                     GRLSLDQVGVIAQHAGSGSDEHYAELASVATVNQLRTAIRLEPRPEPGPKPEPQRSIT
                     KITGAEFTTYRITVPNIEAAQVDAALQSHREALISENTRDHGPDTPGDDHQRPSQGGS
                     KCRPPFPTLTDAFLRMVESAWDSEVARRPHGQHATVIVHLDLDKRAAWPHLGPLLSDA
                     DRQYLLCDASCEVWLQRRGQLIGSGRTTR"
     gene            complement(745..903)
                     /locus_tag="VHH53_05460"
     CDS             complement(745..903)
                     /locus_tag="VHH53_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289636.1"
                     /translation="MVSIAPSGVEVDLSRLERLEVLFGQLEELSGQRNAIDGRIVQIV
                     AELDHDNL"
     gene            complement(1005..>1504)
                     /locus_tag="VHH53_05465"
     CDS             complement(1005..>1504)
                     /locus_tag="VHH53_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX2289637.1"
                     /translation="LSRLRTERTLFDECDFRGVDFTESEHVGSAFRNCRFERATLHHS
                     MFRQCSMLGSVFTGSRLRPLALVDVDLTLAVLGGCDLRGVDLAGCRLREAGLVDTDLR
                     KSVLRGADLSGARLQNVRLDEADLRGARTDATLWTTASLRGVKIDVDQALAYAAAHGL
                     NIHGE"
BASE COUNT          271 a          521 c          466 g          246 t
ORIGIN      
        1 cgcgtcgtgc ggcccgaccc gatgagctgc ccgcggcgct gcagccacac ctcacaactc
       61 gcgtcacaca acaaatattg ccgatcggcg tcggagagca gcggacccag gtgcggccat
      121 gcggcgcgct tgtcgaggtc cagatgcacg attacggtcg cgtgctgccc gtgcgggcgc
      181 cgcgccacct cactgtccca ggcggattcc accatgcgca gaaacgcatc ggtcagtgtc
      241 gggaacggcg gccggcactt cgagccgccc tgcgaagggc gttggtggtc atcgccgggc
      301 gtgtcggggc cgtggtcgcg ggtgttttcg ctgatcagcg cctcacgatg ggactgcagc
      361 gcggcgtcga cctgggccgc ctcgatattg ggcacggtga tccggtaggt ggtgaactcc
      421 gcaccggtga tcttggtgat cgaacgctgc ggctcgggct tggggccggg ctcagggcgc
      481 ggttcgaggc ggatcgcggt gcgcagctga ttgaccgtgg ccaccgacgc cagctccgca
      541 taatgctcat cagagccgga accggcgtgc tgggcgatca ctccgacctg atctaacgac
      601 aaccgcccct gcctcatctc ggcggtgcac cggggcaact catcgaaccg atccgcaatc
      661 gcggccaccg tcttggccgt cgcatccgac agcccgagtt tccaacccac caacgccttg
      721 accgagcgcg ccccggtcat cccctcacag gttgtcgtgg tcgagctcgg cgacgatctg
      781 cacgatgcgc ccatcaatgg cattgcgctg acccgaaagc tcctccaatt gcccaaacaa
      841 cacctccaga cgctcgaggc ggctcaaatc cacctcaacg ccggacggag cgatcgaaac
      901 cataaccgca tcatgacagc acccaccgac aaatcacggc ccatccacaa actgcctgtg
      961 aaaacggtga atattgctgc gccgcagcgg tttcgatcag cgattcactc gccgtggatg
     1021 ttcaggccat gcgccgcggc gtaggccaag gcctggtcca cgtcgatctt cacgccgcgc
     1081 aggctcgcgg tggtccacaa cgtggcatcc gttcgggcgc cgcgcagatc ggcctcgtcc
     1141 aatcgaacgt tctgcagccg tgcgccgctc agatccgcgc cgcgcagtac cgacttgcgt
     1201 aggtcggtgt cgacgagtcc ggcctcgcgc aaccggcacc cggccagatc cactccgcgt
     1261 aggtcacatc cgccgaggac cgccaacgtc agatcgacgt cgaccagggc cagcggccgc
     1321 agccggctgc cggtgaatac cgacccgagc atgctgcact gccgaaacat gctgtggtgc
     1381 aatgttgccc gctcgaagcg acagttacgg aatgccgagc cgacgtgctc tgattcggtg
     1441 aaatcgacac cgcggaagtc gcactcgtcg aacagggtcc gctcggtgcg cagcctgctg
     1501 aggt
//
LOCUS       DATADN010000417         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1112432, whole genome shotgun sequence.
ACCESSION   DATADN010000417 DATADN010000000
VERSION     DATADN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112432"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /gene="sigM"
                     /locus_tag="VHH53_05470"
     CDS             <1..564
                     /gene="sigM"
                     /locus_tag="VHH53_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HEX2289638.1"
                     /translation="GDPLAFSELVRRHRDRMWAIALRTLGDPDEAADAVQDALISAYR
                     CAGGFRGQAQVTTWLHRIVVNACLDRIRRNQARPTAPLPDWGLEEPALTLDAMAARES
                     RLAVDDALRSLPAEQRAAIVLVDVEGYSVAETAQLLGIAEGTVKSRCARGRARLAVLL
                     GHLRNPDANANVSCAAVAGRQHRGKGR"
     gene            561..1388
                     /locus_tag="VHH53_05475"
     CDS             561..1388
                     /locus_tag="VHH53_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289639.1"
                     /translation="MTGTEGPIRPGDPPQRSAAQSPEWSLDLLADLHAGALTEQAATS
                     LRARAIADPQARTVLAALDATVADLAALPTQRSAEIPDAVAQRLDAALAAEVQRADRP
                     PAHLPDRARTGRYRAARQLVTRPAGRRRARARWVAAGVLAAAVIGVTVAGAMIGVMSG
                     GTPRAGDALGAAAVSPPTPLVLSSGDLSGALSPAWGARDYGALSRPEALQGCLDANGV
                     PARDKPLGAREVTLDGRRGVILVLPSTQSTQFRLLVVGLGCGPGNPARMADAVARHR"
BASE COUNT          238 a          551 c          565 g          230 t
ORIGIN      
        1 ggagatccac tggcgttcag cgagctcgtg cgccggcacc gagaccggat gtgggccatc
       61 gcgctgcgga ccctgggcga tcccgacgag gccgccgacg cagtgcagga cgcgttgatc
      121 tcggcgtatc gctgcgccgg cgggttccgt ggccaagccc aggtcaccac ctggctgcat
      181 cggatcgtgg tcaacgcctg cctggaccgg atacgccgca accaggcccg ccctaccgcg
      241 ccactgcccg actggggtct ggaggagccg gcattgacac tggacgcgat ggcagcccgg
      301 gaatcgcggc tggcggtcga cgatgcgctg aggtcactgc ccgccgagca acgtgccgcg
      361 atcgtcctgg tcgacgtcga gggatactcg gtggctgaga ccgcccagct gctgggcatc
      421 gccgagggaa ccgtcaagag ccgctgcgcc cgcggacgcg cccggctggc ggtactcctt
      481 ggacatctac ggaaccctga tgcgaatgca aacgtctcat gcgctgccgt ggcggggcgc
      541 cagcatcgag ggaagggacg atgaccggca ccgaaggccc gatacgcccg ggcgatccgc
      601 cgcaacgatc ggcagcacag tctccggagt ggtcgctgga cctgctcgcc gacctgcacg
      661 cgggtgcgct caccgagcaa gctgccacgt cgttgcgggc tcgagcaatc gcggatccgc
      721 aggcccgtac cgtgctcgcg gccctggatg ccacggtggc cgatctggcg gcactgccaa
      781 cccagcgctc agccgaaata cccgacgccg tggcgcaacg actcgacgcc gcgctggccg
      841 ccgaggtcca gcgagctgac cgccctcccg ctcacctgcc ggaccgggcg cggaccgggc
      901 gttaccgcgc cgcccggcag ctggtgacca ggccggctgg ccggcgccgc gcccgcgccc
      961 gttgggtcgc cgctggtgtg ctggctgcgg ccgtgatcgg ggtgacggtg gccggtgcga
     1021 tgatcggtgt gatgagcggg ggcacgccgc gggccggtga cgcattgggc gccgcagcgg
     1081 tctctccgcc cacgccgctg gtgctgtcca gcggtgacct cagcggtgcg ctgagtcccg
     1141 cgtggggcgc acgcgactac ggtgcgctgt cgcggcccga agcgctgcag ggctgcctgg
     1201 acgccaacgg tgtaccggcg cgcgacaagc cactgggagc ccgggaggtc accctcgacg
     1261 ggcggcgcgg tgtgatcctg gtgctgccca gcacgcaatc cacccagttc cggctgctcg
     1321 tggtgggctt gggctgcggc cccggcaacc ccgctcggat ggccgacgcc gtagcgcgcc
     1381 accgctgacg ttgggtgccc ttcaaggact accaacccac ccggcaccgt ctctaaacga
     1441 ctaccaaccc gtcccgaaaa ccagcgagaa cactcgacgt tggtggctgt tgagagcacc
     1501 gattgcctag gttggacgta cttggagagc gggcaactgg ctcgggaaca tcggtccgta
     1561 ggatcgtgtt gtgtccaatg tgag
//
LOCUS       DATADN010000418         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_792898, whole genome shotgun sequence.
ACCESSION   DATADN010000418 DATADN010000000
VERSION     DATADN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792898"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VHH53_05480"
     CDS             <1..114
                     /locus_tag="VHH53_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX2289640.1"
                     /translation="TGPDIALDLVESRSTPKGVTIQIYRPTGPPQYATATT"
     gene            complement(152..291)
                     /locus_tag="VHH53_05485"
                     /pseudo
     CDS             complement(152..291)
                     /locus_tag="VHH53_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611217.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            469..1635
                     /locus_tag="VHH53_05490"
     CDS             469..1635
                     /locus_tag="VHH53_05490"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2289641.1"
                     /translation="MTSALQDLLERHVASGTVPGVVASLGTAELKIVAAGAMSVGGAP
                     MPADAIMRIQSMTKAVTTVAALRLVEAGRLRLDEPVERWLPELADRQVLKSPTAELAD
                     TSPAVRAITLRHLLTNTSGYGMVLTECPLQRAMADSETEAGVGPVTLGAEEWLARLAA
                     LPLAFQPGEGWRYHHSFGILGILIARVAGRPLQDQLADDLLGPLGLRDTGLWVSAGSV
                     ARLPASYRHTDSGLVELEPAAGGFYAGHPPFDVSHAELVSTVRDYTRFARMLADGGRF
                     EGRQIVSEEHLRLMTSDQVPAIAKTPDSFFPGFWDGTGWGFGVGVDTEGGRAGRYGWS
                     GGLGTNFFVDPDGTVGVLLTQVEMGERMQLLLDEFQVLPEAARRSKTSTRQLSP"
     gene            complement(2251..2814)
                     /locus_tag="VHH53_05495"
     CDS             complement(2251..2814)
                     /locus_tag="VHH53_05495"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2289642.1"
                     /translation="MAPGVRGHGGRPGVPPPLGRTGRRPHRHRHLSDVETAMDIHFNH
                     TEPVAAPAEALFEVITDYAGYSRFNPAVVKMTVVARHENGAEFVAKCKTRIAREVRAF
                     DRYQRHGDLVIKRTYGPESAAQSTWTIRPVDGGPLHPHHRRVEHDAIRARSRDEAPPA
                     AALLRHQLQSFIQEAQRRANATRTQTD"
     gene            complement(2721..3317)
                     /locus_tag="VHH53_05500"
     CDS             complement(2721..3317)
                     /locus_tag="VHH53_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX2289643.1"
                     /translation="MERTPRADAADVLAGVGRHWGWVLAFGIVTLLAGLLALAWPGRT
                     VVVLALLFGVQLVVAGIFRFVAALATDDETGATRVLLALLGVLSFIVGLYALRHILVT
                     IATLALLLGIFWIVNGAIEVFTALSHRRMPGRGWTILMGLLSAVAGVVILVYPGISLV
                     TLAVVLGVWLLVFGAMEVGLAFRLRSVGQGAARIATAT"
BASE COUNT          590 a         1100 c         1153 g          539 t
ORIGIN      
        1 accggcccgg acatagcgct cgacctggtg gaatcgcggt ccaccccgaa gggggtaacg
       61 atccagatct accggcccac cggccccccg cagtacgcaa cggccacgac gtaaagcagg
      121 tgacctaggt gccgcagtca gtgagcgtcg ctcattggtg ggtgcgggct gcagcgtcga
      181 ccgctgggag ctggtcgccg agcgcggagt gtcgccgctg gtggttgtag tctgccttcc
      241 agtcgctgct caccaccggc gcctggccag tgaccagaac atgttgatgt tgcgagcagc
      301 tcgccgcatg gcgacgccag gtgccgcggt ccgacctgcc cgagggcgtc gagatgctcg
      361 acgtggcgat ggctgcatat gtcgtcgcgg tccggagaga gccgttctcg ggtcgctacg
      421 accaggcgag ctgagacgac cgaatgagtg gctgggagat gtcgaggaat gaccagtgcg
      481 ttgcaggacc tgctcgagcg gcacgtcgcg agtggcaccg tcccgggtgt cgtcgcgagc
      541 ctgggcacag cggaactcaa gatcgtggcc gccggcgcga tgtccgtcgg cggtgcgccc
      601 atgcccgccg acgcaatcat gcgcatccag tcgatgacca aggccgtcac tacggtcgcg
      661 gccctgcgcc tggtcgaggc cggacgcctg agactcgacg agccggtgga acggtggctg
      721 cccgaactgg cggatcgaca ggtgctgaag agtcccaccg ccgagctggc cgacacatca
      781 ccggccgttc gggcgatcac gctgcggcat ttgctgacca acacctccgg ctacggcatg
      841 gtgctaacag agtgtccgct gcagcgggcg atggctgaca gcgaaaccga agctggcgtc
      901 ggccccgtga cgttgggggc cgaggagtgg ctggccaggc tggcggcact gcccctggcc
      961 ttccagccgg gcgagggctg gcgctaccac cactccttcg gcatcctcgg aatcctgatc
     1021 gctcgagtgg ccggccggcc gttgcaggat cagctggccg acgacctgct cggacccctc
     1081 ggcctccgcg acaccggact gtgggtgtcc gcaggcagcg tcgcccggct gccggccagc
     1141 taccggcaca ccgactccgg tctggtggaa ctcgaaccgg ccgccggtgg cttctacgcc
     1201 gggcacccgc cgttcgacgt gagtcacgcc gagctcgtct cgacagtccg cgactacacc
     1261 cgcttcgccc ggatgttggc cgacggcggc cggttcgagg ggcggcagat cgtgtcggag
     1321 gagcatctcc ggctgatgac cagcgaccag gtgccggcta tcgccaagac gccggacagc
     1381 ttcttcccgg gcttttggga cggtaccggc tggggcttcg gcgtcggtgt cgacaccgaa
     1441 ggcgggcggg ccggccgcta cgggtggtcc ggcggactgg gcaccaactt cttcgtcgac
     1501 cctgacggca ccgtaggtgt cctgctgacc caggtagaga tgggcgaacg gatgcagctc
     1561 ctgcttgacg agttccaagt cctgcctgaa gcagcacgac gctcgaagac gtcgaccaga
     1621 caactttcgc catagcgcgg cgagtcgtca ggatcggttg tcgcagaagg ttcgtctcta
     1681 cgacagtgac cctcccccgc cgccgaagcc gcagtcggtg tccagaaccc gttccgaaat
     1741 caaggtcgca caatcagcca cgtcacaggg ttccgcgacg ctagcggcgc agaatcgctg
     1801 ctcgggagcc cgtgtcacca cctgtcgcta cataggcgca gctagacgat gcgatgagat
     1861 gctcagtgag cgccagggag agggcatgaa ggagagcgtt tagctgaacg ccaactgagc
     1921 gtttccctgc cgggtctacg gtcaggaacg acctgggaga acggcgtgta gcacagcggt
     1981 tcccggggtt ttggctgaca cggaagaggt cacaggttca aaccctgtcg cgcccaccaa
     2041 caacacgctg accagccgaa acgctggtca gttcgctgcc tgggccggtt cggcggcccg
     2101 tgcaccacaa tcagggcaca ctcgatggat ggtgcagcgg atgacaccac ttaacctgtt
     2161 aaccgtttga ggtcgcttca tcctggggcg acacggccgg agccgcggca ggtctagatg
     2221 ctcaagcgtc caggatcagt tgaccggcgg tcaatctgtc tgagttcttg tcgcgtttgc
     2281 gcgtcgctgc gcctcttgga tgaatgactg aagttgatgc cgtagaagag ccgccgcagg
     2341 aggggcttca tcacgagacc tggcacgaat ggcatcgtgc tcgacgcgtc gatggtgagg
     2401 gtggagtggg ccgccatcga caggacggat tgtccacgtc gactgagccg ctgattcggg
     2461 accgtaggtc cgtttgatga cgaggtctcc gtggcgttgg tatcggtcga acgcacggac
     2521 ctccctggcg atcctggtct tgcacttggc gacgaactcg gcgccgttct cgtgcctggc
     2581 gaccaccgtc atcttgacca ccgctgggtt gaagcgtgaa tagccggcgt agtcggtgat
     2641 cacctcgaac agggcctcgg ccggcgcggc caccggttcg gtgtggttga agtggatatc
     2701 catcgctgtc tcgacgtcac tcaggtggcg gtggcgatgc gggcggcgcc ctgtccgacc
     2761 gagcggaggc ggaacgccag gccgacctcc atggccccga acaccaggag ccataccccg
     2821 agaacgaccg ccagagtcac gagcgagatc cccgggtaga ccagtatcac cacgccggcg
     2881 accgcgctga gaagccccat gaggatcgtc cagccgcggc ctggcatccg acggtgcgac
     2941 agcgcggtga agacctcgat ggcgccgttg acgatccaga agatgcccag cagcagcgcc
     3001 agcgtggcga tggtgaccag aatgtgccgc agggcgtaca gcccgacgat gaacgacagc
     3061 acccccagca gggccagcag cacccgggtc gcgccggtct cgtcgtcggt cgccagcgcg
     3121 gccacgaacc ggaagatccc ggccaccacc agctggacgc cgaacagcag ggctaggacc
     3181 acaacggttc gacccggcca ggcgagtgcg agcaggccgg cgagtagcgt aacgatgccg
     3241 aaggcgagca cccagcccca gtggcggccg acgccggcga gcacgtcggc ggcgtcggcc
     3301 cgtggcgtcc gctccatcga agcctccctt ccacgagccg tcggccgctg gcgccgtgtc
     3361 gcggccatgc tcgcgatgct cg
//
LOCUS       DATADN010000419         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1112557, whole genome shotgun sequence.
ACCESSION   DATADN010000419 DATADN010000000
VERSION     DATADN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112557"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..>1761)
                     /locus_tag="VHH53_05505"
     CDS             complement(73..>1761)
                     /locus_tag="VHH53_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HEX2289644.1"
                     /translation="AQIGHASSLHAGQAVPDGQSWHGSPARRTDVDYRMIPATRCGPL
                     RRVSYSSGQALTGLLLAAPLAFAIPATLLTKVPTLAGLLGPGQSLASWIFYRNALVIS
                     LVLFFGIMLLGLAAVATIPRLLNLAITPDTVYPLYGVRYWVQGTIGRLTNSKFYLRLF
                     GDSSYVVGYLRYLGYDLSRVEQTGSNVGNELKHDSPHLSFVGTGTMLSDGVSINNADY
                     SSTSFRVSPVSLGSRSFFGNNIAYPSQGRTGDNCLLATKAMVPIDGKIREGVGLLGSP
                     CFEIPRSVHGGSTVHSGAELYRGLAAKNRYNLATIGLYLVVHWIRFFVAVLFALTALD
                     LYDQFSFAVVPVTVIVVLLFNVSYSVLVERAIGRFRPLSPRSCSIYNPYYWWHERLWK
                     LEAVVPWPGTPFRNLIWRLLGVRIGRGIFDDGCYIPEKTLVAIGDRCTLNASSVIQCH
                     SQEEGIFTSDRTTIGAGCTVGINAFIHYGVTLGDGAVLDADSFLMKGEQIASHTRWHG
                     NPARQMRNVDPVPRTAVTPAVTAAPVIAALKALKRRHPVPHCRCSRWRGTCGAA"
BASE COUNT          348 a          548 c          586 g          279 t
ORIGIN      
        1 gtcacatgga tgcttgtagc aattgtggga ctcggcaaac tgtcatatgg atgcttgtag
       61 cggttgcgcg ggtcatgctg cgccgcaggt gccgcgccag cgagaacagc ggcagtgggg
      121 aactgggtga cgccgcttca atgccttcaa tgccgctatc accggtgctg cggtgacggc
      181 gggggtgacc gcggtgcgag gcaccggatc cacgttccgc atctggcggg ccggattgcc
      241 gtgccaccgg gtgtgcgacg cgatctgctc gcccttcatc aggaaggagt cggcatcaag
      301 caccgcgccg tcgcccaacg tcacgccgta atggatgaag gcgttgatcc ccacagtgca
      361 gccggcaccg atcgtggtcc ggtcagaggt gaagatgcct tcctcctgcg agtggcactg
      421 gatcacgcta ctggcattga gcgtgcaacg gtcgccgatg gcgaccagcg tcttctccgg
      481 gatgtagcag ccgtcgtcga aaatccctcg cccgatgcga acgcccagca gccgccagat
      541 caggttcctg aacggggtgc ccggccacgg caccaccgct tccagcttcc acagccgttc
      601 gtgccaccag taataagggt tgtagatcga gcaggaccgc gggctcaagg gacgaaacct
      661 gccaatagca cgttcgacga gcacggagta gctgacgttg aacagcagga cgacgatcac
      721 ggtcaccggg accaccgcga aactgaactg gtcataaaga tccaaggcgg tcagcgcgaa
      781 caacacggca acgaagaaac ggatccagtg cacgaccagg tacaacccga tcgtggccag
      841 gttgtacctg ttcttggcgg cgaggccgcg gtacagctca gcaccgctgt gcactgtgga
      901 accgccgtgc accgatcgtg gaatctcaaa gcacggtgag ccaagcagtc cgacgccctc
      961 ccggattttc ccgtcgatgg gcaccatggc cttggtggcg agcaggcagt tgtcgcccgt
     1021 gcggccctgc gacgggtagg cgatgttgtt cccgaagaaa ctgcgggacc cgagcgacac
     1081 cggcgacacc cggaacgacg tgctggagta gtcggcattg ttgatcgaca cgccatcgga
     1141 gagcatcgtg cccgtaccca caaagctcag atgcgggctg tcgtgcttga gctcgttgcc
     1201 gacattcgac ccggtctgct cgacccgcga caggtcatac ccgaggtagc gcaggtagcc
     1261 gacgacgtag gagctgtcgc cgaagagccg caggtagaac ttcgagttgg tcaggcgccc
     1321 gatcgtcccc tggacccagt agcgcacgcc atacagcggg tataccgtgt ccggcgtgat
     1381 ggccaggttc agcaggcgcg gaatggtggc cacggcggcg aggccgagta gcatgatccc
     1441 gaagaacaac accagcgaga tcaccagcgc gttgcggtag aatatccagc tcgccaggga
     1501 ctgacccggc cccagcaagc cggcgagcgt tggcaccttg gtgagcagcg tggccgggat
     1561 ggcgaaggcg agcggggccg ccagcaacag cccggtcagc gcctgaccgc tgctgtacga
     1621 aaccctccgc agggggccgc agcgggtcgc agggatcatc cggtagtcga catcggtccg
     1681 ccgcgccggc gacccgtgcc agctctggcc atcgggaacg gcttgacccg cgtgcagcga
     1741 ggaggcgtgg ccgatctggg c
//
LOCUS       DATADN010000420         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_190725, whole genome shotgun sequence.
ACCESSION   DATADN010000420 DATADN010000000
VERSION     DATADN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190725"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1851
                     /locus_tag="VHH53_05510"
     CDS             <1..1851
                     /locus_tag="VHH53_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX2289645.1"
                     /translation="AGRWRSRWVTTPITGGDSAADEEVDVVVIGSGAGGAPLTSVLIS
                     AGRQVLILEKGPLLRTQYQAPDGVSDFKRDETFATGSEKRITIPGMANLGDSFYSSHV
                     EPDLNDEPHVYEGEDGLDRATIEGYTAQVVGGGTQLYGGVSLRFTPDDFRLASFNEGR
                     TDLRDDPAGDVRREARDWPISYDELEPYYCKAENLVGINGTRDGQLKPASEEHYQTPL
                     EPNPISAYAAAGMDALGMRRYRTPLAVITADHAPSGRTVPNDRDTIKTAFVNRYGDPL
                     GLKSNTWVSLLSPLVGKPGFELRANCVVTHLEADGTKVTRVHYRDPTGAARSVKAKTV
                     VVACSAIESARLLLLSGQQDPAFAQRANPNGLVGAYFLTHCFGGGQCVVPLAGRADKS
                     KTLDSDWATDHCATPQFLQANGLWAGGAIYNNTSDGALPISLARTWAAADMDSMWKGY
                     LSDTKLVGKGFENYLELNFGRRLSVAFMANQVPQRGNRIELHPRVQDKWGRPVAYIRK
                     TWHSHDRALMRTFATQCHQILERGGGINETISSGSVEGSVVRIANHVLGGARFGTDPQ
                     DSVLDSDCRVWGLDNLYVTDGAFMPTSGGANPTLTIQANSFRVGDILAGR"
     gene            1856..2995
                     /locus_tag="VHH53_05515"
     CDS             1856..2995
                     /locus_tag="VHH53_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289646.1"
                     /translation="MDTRFEQIFTQPENEIFRVVFFPDRIYHARYLNATRSSRYRYNV
                     TEVRGGPGITVMKGEVYLGGSRLCGMLRVEYAASRLVEQAREFDRRLGPTIKAWVRVI
                     PADSAKSGETTITLHWDSLIGAYATEIWETLEPPAGSSHDYRVLALMGRDAPITRASE
                     LSPALADLPSLRQIAVAFREDNRLFPTGAPIANVQWDNAYLRSHQEPRTPQPSSPENT
                     ILDQNYLLNFQRGFFIQDAAQVTPVNYRNAMMNGNDPERRDDNIVQMRWLLQRELGGD
                     LIFFHEVTVPPGAVEGTHRHIGSEELYYIVSGSGIAYMADRDDPTTSDRPLVQRPVYD
                     LDPVQCRELQVQAGSMIYTKSGGVHGIRNPGTEPLKFVAFLYQTT"
     gene            3006..>3360
                     /locus_tag="VHH53_05520"
     CDS             3006..>3360
                     /locus_tag="VHH53_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289647.1"
                     /translation="MAQLVHTDSIFRVDPGVTPPYDTDNPLLRLLGLVETTRLVGARE
                     VYEHNSLQYLFPLTWLQLWGPSAPETDNLDPAQQDTKTDFQKQNVRDFRLGDTRAVRG
                     WAVVGGQRVPMLQLSY"
BASE COUNT          604 a         1092 c         1133 g          531 t
ORIGIN      
        1 gccgggcgtt ggaggtcccg ctgggtcaca acaccgatta ccgggggtga cagcgcggct
       61 gacgaggaag tcgacgtagt ggtgatcggc agcggcgccg gtggcgcgcc gctgacgtcg
      121 gtgttgatca gcgccggtcg gcaggtgctc atcttggaga aaggaccgct gctgcgcacc
      181 cagtaccagg cgccggatgg ggtgagcgac ttcaaacgtg acgagacgtt cgccaccggt
      241 tcggaaaagc gcatcacgat ccccgggatg gccaacctcg gcgacagttt ctactccagc
      301 cacgtcgagc cggacctcaa tgacgaaccg cacgtctacg agggcgagga tggcctggat
      361 cgggcgacga tcgagggcta cacggcccag gtggtcggtg ggggcactca gctctacggt
      421 ggtgtctcgc tgcggttcac acctgacgac ttccggctcg cctcgttcaa cgaaggccgg
      481 acggacctgc gcgacgaccc ggcaggcgat gtgcggcggg aggcccgcga ctggccgatc
      541 agctacgacg agctcgaacc gtattactgc aaggccgaga acctcgtcgg catcaacggc
      601 actcgcgacg gccagctcaa accggcgtcg gaggagcact accagactcc gctggagccc
      661 aatccgatca gcgcctacgc cgccgcgggc atggacgcgt tgggaatgcg gcgctaccgc
      721 acaccgctcg cggtcatcac cgccgaccac gcaccgagtg gacggacggt ccccaacgac
      781 cgggacacca tcaagaccgc cttcgtgaac cggtacggcg acccgctcgg gctgaagtcc
      841 aacacgtggg tatcgctgct gtccccgctg gttggcaaac caggcttcga gttgcgggcc
      901 aactgcgtcg tgacccacct ggaggccgac ggcacgaagg tgacccgcgt gcattaccgg
      961 gacccgaccg gcgcggcccg ttcggtgaag gccaagactg tggtggtggc ctgctcggcc
     1021 atcgagtcag cgcgactgtt gctgttgtcg gggcagcagg acccggcgtt cgcccagcgt
     1081 gccaacccga acgggctggt cggcgcgtac ttcctgaccc actgtttcgg cggcgggcag
     1141 tgcgtcgtac cgctggccgg ccgggcggac aagtccaaga cgctcgacag cgactgggcg
     1201 accgatcatt gcgccacccc gcagttcctg caggcaaatg ggttgtgggc cggcggcgcg
     1261 atttacaaca acacctcgga tggggcgctg ccgatctcgc tggcccgaac gtgggcggcg
     1321 gcggacatgg acagcatgtg gaagggctac ctgtccgaca caaagttggt gggtaagggt
     1381 ttcgagaact acctcgagct caacttcggc cgaaggctgt cggtggcctt catggccaac
     1441 caggtgccgc agcgcggcaa ccgcatcgag ctgcatccca gggtccagga caagtggggc
     1501 cgaccggtgg cctacatccg caagacctgg cacagccacg accgggcgct gatgcggacg
     1561 ttcgctaccc agtgccatca gattctcgag cgtggcggcg ggataaacga gaccatcagc
     1621 tcgggttctg tcgagggttc agtggtccgc atcgccaacc acgtgctcgg cggcgcccgg
     1681 ttcgggaccg atccgcagga ctcggtactg gactccgact gccgggtctg gggcctggac
     1741 aacctctacg tcacggacgg ggcgttcatg ccgacctccg gcggtgccaa ccctacgttg
     1801 accatccagg cgaactcatt tcgcgtcggc gacatcctgg cagggaggtg accgggtgga
     1861 cacccggttc gagcagatct tcacccagcc cgagaacgag atcttccggg tggtgttctt
     1921 cccggaccgg atctaccacg cgcgttacct caacgcgacg cgctcgtcgc gctaccggta
     1981 caacgtcacg gaggtccgcg gcggtccggg gatcaccgtg atgaagggcg aggtatacct
     2041 gggtgggtcg cggctgtgcg ggatgttacg cgtggagtac gcggccagcc ggctggtcga
     2101 gcaggcacgc gagttcgacc gccggctggg gccgacgatc aaggcctggg tgcgggtgat
     2161 accggctgac tcggcaaaga gcggcgagac gaccatcact ctgcattggg attcgctgat
     2221 cggcgcttat gccactgaga tctgggagac gctcgagccg ccggccggtt ccagccacga
     2281 ttaccgggtg ctggcgctga tgggccggga cgctccgatc acccgcgcct ccgagctgag
     2341 cccggcgctg gctgacctgc cctcgctgcg ccagatcgcg gtggcttttc gggaggacaa
     2401 ccggttgttc cccaccggag caccgattgc caatgtgcag tgggacaacg cctatcttcg
     2461 ttcgcaccag gagccgcgga cgccgcaacc cagctcgccg gagaacacca tcctcgacca
     2521 gaactacctg ctgaacttcc agcgcggctt cttcatccag gacgcggcac aggttacgcc
     2581 ggtgaactac cgcaacgcca tgatgaatgg gaacgacccc gagcggcgcg acgacaacat
     2641 cgtccagatg cgctggctgc tgcaacggga actgggtggc gacctcatct tctttcacga
     2701 ggtcacggtc ccgcccggtg cggtggaggg aacgcaccgg cacatcggct cggaggagct
     2761 gtactacatc gtgtccgggt cgggaatcgc ctatatggcc gaccgcgacg acccgacaac
     2821 cagcgaccgg ccgttggtgc agcgaccggt gtacgacctc gatccagtcc aatgccggga
     2881 actgcaggtc caagccggca gcatgatcta caccaagagc ggcggggtgc acgggatccg
     2941 caatcccggc acggagccgc tgaagttcgt cgccttcctg taccagacca cctgaagggg
     3001 gaaccgtggc ccagctggtg cacacggaca gcatcttccg ggtcgatccg ggggtgacac
     3061 cgccgtatga caccgacaac ccgctgctgc gcctcctcgg cctggtggag acgacccggc
     3121 tggtgggagc tcgggaggtg tacgagcaca actcattgca gtacctgttc ccgctgacct
     3181 ggctgcagct gtgggggccc agcgccccgg agaccgacaa cctcgatccg gcgcagcagg
     3241 acaccaagac tgacttccag aagcagaacg tccgcgactt ccggctcggt gacacccgcg
     3301 cggtgcgggg ctgggctgtg gtgggtggtc agcgggtgcc gatgctgcag ctgtcctacc
//
LOCUS       DATADN010000421         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_793092, whole genome shotgun sequence.
ACCESSION   DATADN010000421 DATADN010000000
VERSION     DATADN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793092"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VHH53_05525"
     CDS             complement(<1..936)
                     /locus_tag="VHH53_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289648.1"
                     /translation="MLLTSAHRLAEPRNQVVAELVADQPAGSTVAVDVRGTRDNGSWT
                     EWVPASTEEPAMFDAAVTSVQTRVLLRGGDGGASPRVRSVRLSAHTIAQTLAAVPLSA
                     RSYRVFATREGLVGGTTANGHVIRPRDHFVALPSRQALAPRGSGDYTVKVCSDRGRCE
                     WAPVWDVGPWNTTDDYWNSPQTRQSWRDLPQGRPQAQAAYSDGHNGGRDQFDRQVRNP
                     AGIDLADGTFWDGLGLRNNSWVTVTYLWADGAPLGIVRMPLLHVRSGPGTDHPPVGLA
                     AQRATLRVECTVIGQAVTGTHGVTDRWLRIGTQQYV"
     gene            complement(993..1211)
                     /locus_tag="VHH53_05530"
     CDS             complement(993..1211)
                     /locus_tag="VHH53_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289649.1"
                     /translation="MRLPARRCRRVHIAVVAIAVLAAIAVVTFPAIARAGAQGAENGA
                     AAGAGDTTWFAGLSPDWDDTNVRSEAGA"
     gene            complement(1368..>1587)
                     /locus_tag="VHH53_05535"
     CDS             complement(1368..>1587)
                     /locus_tag="VHH53_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEX2289650.1"
                     /translation="PDPKWGERVTAVVVPVDPGRPPDPGALRAAARRLLTGAQTPKEI
                     VLFDALPLRGVGKPDRSAVRAMLAARDR"
BASE COUNT          225 a          548 c          569 g          245 t
ORIGIN      
        1 cacgtactgc tgcgtgccga tccgcagcca ccggtcggtc acgccgtggg tgccggtgac
       61 ggcctgcccg atgacggtgc actcgacacg cagggtggcg cgctgcgctg ccaggcctac
      121 cggcgggtga tcggtgccag ggccgctgcg gacgtgcagc aacggcattc gcacgatacc
      181 cagcggcgcg ccatcagccc acaaataagt gacggtgacc cagctgttgt tgcgcagccc
      241 cagaccgtcc cagaatgtgc cgtcggccag gtcgatcccg gctgggttgc gcacctgccg
      301 gtcgaattgg tcccggcccc cgttgtggcc gtcgctgtat gcggcctgcg cctggggtcg
      361 gccctgcggc aggtcgcgcc aggattggcg ggtttgcggg gagttccagt aatcgtcggt
      421 ggtgttccac gggccgacgt cccacaccgg cgcccattcg catcggccgc ggtctgagca
      481 gaccttcacc gtgtagtcgc ccgaaccacg gggggccagt gcctggcgcg agggtagcgc
      541 gacgaagtgg tcgcgcgggc ggatcacgtg accgttggcg gtggtgcccc ctaccagacc
      601 ctcgcgggtc gcgaacaccc gatagctgcg ggccgagagc gggacagcgg ccagggtctg
      661 ggcgatggtg tgcgcgctga gccgcaccga ccgcacccgc gggctcgcgc caccgtcacc
      721 accgcgcagc agaactcggg tctgtaccga cgtcaccgca gcgtcgaaca tcgcgggttc
      781 ctcggtgctg gcgggcaccc actcggtcca cgagccgttg tcccgggtgc cgcggacgtc
      841 gacggccacg gtcgatccgg ccggctgatc agcgacgagt tccgccacca cttggttgcg
      901 gggctcggcg agccggtgcg ccgaggtgag cagcatgcct tgtggcgggc cggtccgggt
      961 gctcgccggg cccggggcat cgccgttcag gctcaggcac ctgcctcgga acgcacgttc
     1021 gtgtcgtccc agtcgggact caacccggca aaccaggtgg tgtccccggc cccagccgcg
     1081 gccccgttct cagcgccctg cgctccagct cgtgcaatgg ccgggaaggt caccacagca
     1141 atcgccgcga gcactgcgat ggcgacaacg gcgatgtgga cgcggcggca gcgacgagcc
     1201 ggcagcctca cgggacactc ctgagcgtag gaggcaggtg tttccgctgt gactgatgtg
     1261 gttggtgggg atgttggtac ccaccgaacc cgcatctatg cgtcgccgtc catacctaga
     1321 gtgggtgaac ctgtactgcc tgtgttcagt gaggtccgcg tggccggtca gcgatcccgt
     1381 gcggcgagca tggctcggac cgcgctgcgg tcgggcttgc ccacgccgcg cagcggaaga
     1441 gcgtcgaaca agacgatctc cttgggggtc tgcgcgccgg tcaacaggcg gcgggcggca
     1501 gcgcgcaacg cgccgggatc cggcggccgg cccgggtcta ccggtaccac cactgcggtg
     1561 acccgctcac cccacttcgg atccggc
//
LOCUS       DATADN010000422         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1244077, whole genome shotgun sequence.
ACCESSION   DATADN010000422 DATADN010000000
VERSION     DATADN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244077"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            665..1795
                     /locus_tag="VHH53_05540"
     CDS             665..1795
                     /locus_tag="VHH53_05540"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX2289651.1"
                     /translation="MGPVPVASGLPLREYVLVGLTAAVVTFLLTGLVRQLALRTGAVA
                     MPRERDVHRAPIPRLGGVAMYGGILGGVLLSHHLPVLRRSFEFSGDVVGVLVAGGIIM
                     LIGALDDRFELDSLTKFAGQVTAAGVLVLFGVQWFIFPVPWNGSALVLGQDQAVLVTV
                     ALAVVLMNAMNFVDGLDGLAAGIGLIAAVAIGAFALQLLDRSGGSVSAYPPTLIAALI
                     AGACLGFLPHNFQPARIFMGDSGAMLIGLMLAAASTSASGRVDLTSYGATDVLALFSP
                     LLVVAAVLFVPLLDLLLAVVRRTRAGRSPFAADKLHLHHRLLEIGHSHRRAVLLIYLW
                     AALLAFGAVALTVFDVYVVVWAIMLGLLIAVLGSMVPHLPAR"
     gene            2101..2310
                     /locus_tag="VHH53_05545"
     CDS             2101..2310
                     /locus_tag="VHH53_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289652.1"
                     /translation="MERRNGPELATLLGLGAATAAILLAGLGVGWLVDEIMDTFPACI
                     LAGLVLGIGGASVFVYTKFTKFLKE"
BASE COUNT          323 a          698 c          793 g          496 t
ORIGIN      
        1 cgtcgccgag ctcaacgagg tgctcggcct caccattcag gtgacccgct gaccgttctg
       61 atcaccagat gggcaggctg ccgaaacggt gatcatagga accggtggag gtgtgggtcc
      121 atctcaacac ggtggaactg aactgatacc tgcgccggca gtccggggtg atcagctacg
      181 tgcaagcgcg ggcggtgggg ctgatgccca aggttctgaa ctgctcgacc gggcgctaca
      241 gcgcgggacc accttccccg cggtataccg tgcgcactgc cgcaaccagg gacggcgggg
      301 atccgcggcg gcctccgact gcttcatgct gcgcagatca ccggttggaa gctgggctat
      361 ctggtacatg gctacctgat cgacgtggcg ttcccgctca gcgggtcgct attgaggtgg
      421 acgggtgagc ctggcacatg acacctgatc ggttcgtcga cgaccggcga cggcagaacg
      481 ccgtggtgat ctgcattgga cagtgctgcg ttcacctggc acgacctggt ggaccgcccc
      541 gacggtgtgg tcaacgagat ccggactgcc ctctcggctc accggtcgcc acgaccaccc
      601 ggcggcaagg gcaggatctg gcctaccggt gggactgaat cacagcgggg ctagcctgcc
      661 tgtcgtggga cccgtgcccg ttgcatccgg actgccattg cgggagtacg tgctggtcgg
      721 tctcaccgct gcggtggtga cctttctgct cactgggctg gtccggcagc ttgcattgcg
      781 cactggtgcg gtggcgatgc cccgcgagcg cgacgtgcac cgtgccccaa tcccgcggct
      841 gggtggggtg gcgatgtacg gcggaatcct cggtggggtc ctgctgtctc accacctacc
      901 ggtgctgcgc aggtctttcg agttctccgg ggacgtcgtg ggagtgctcg ttgccggcgg
      961 catcatcatg ttgatcggcg ccctggacga ccggttcgag ttggactcgc tcaccaagtt
     1021 cgccggccag gtgacggctg ccggggtgct ggtgctcttc ggcgtgcagt ggttcatctt
     1081 cccggtgccc tggaacggtt cggcgcttgt cctgggacag gaccaggcgg tgctggtcac
     1141 tgtcgcgctg gcggtagtgc tgatgaacgc gatgaacttc gtcgatggac tggacgggct
     1201 ggctgccgga atcgggttga tcgccgcggt ggccatcggg gcgttcgccc tgcagctgct
     1261 cgacaggtct ggcggcagtg tgtcggcata cccgccgacg ctgatcgcag ccctgatcgc
     1321 tggggcgtgc ctgggcttcc tgccacacaa cttccagccg gcgcggatct tcatgggtga
     1381 ctccggggcg atgctcatcg gtctgatgct ggccgccgcg tccaccagtg cgtcgggacg
     1441 ggtcgatctc accagctacg gagccaccga cgtgctggcg ctgttctctc cgctactcgt
     1501 ggtggccgca gtgctgttcg tgccactgtt ggacctgctg ctcgcggtgg tgcgccgcac
     1561 ccgcgccggc cgcagcccgt ttgccgctga caagctgcac ttgcaccatc ggctgctcga
     1621 gatcgggcat tcacatcgcc gagcagtgct attgatctac ctttgggcgg ccctgctcgc
     1681 ttttggtgcc gttgcgctga ctgtgttcga tgtctatgtc gtggtttggg cgatcatgct
     1741 cgggctgctt atcgccgtgc ttggttcgat ggttcctcat ttacccgcac gttgactttc
     1801 cctttgccct tgctctgtcc acccacggtc gtgccggcag aagcgcagct aagcgctgag
     1861 ggtgagcaca gggtgtaacg tgctctgtga taacgtctgg ttcgtcccac ctgggcgtgg
     1921 gattgaggtc aggctggtcc catcgacagc gttcatgatc atgacttttc gccatgcgcg
     1981 ctggccctgg actgcgagga gtgctttgag cgatggcggc gggtgacgcc atgcgcccgc
     2041 tgaggtcatg tgcactgagg tcgtgtgtac tgaggttatg tgtggctggg gtaggtggtc
     2101 gtggaacgac gcaacggccc ggagctcgca actttactcg gcctcggggc cgcgaccgcg
     2161 gcgatcctcc tagccgggct gggcgtcggc tggctggtcg atgagataat ggacaccttt
     2221 ccagcgtgca tcctggctgg tctggtgctt ggcatcggcg gtgccagcgt gttcgtctac
     2281 accaagttca cgaagttcct gaaggagtaa
//
LOCUS       DATADN010000423         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_360401, whole genome shotgun sequence.
ACCESSION   DATADN010000423 DATADN010000000
VERSION     DATADN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360401"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VHH53_05550"
     CDS             <1..449
                     /locus_tag="VHH53_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX2289653.1"
                     /translation="GMPDARLVELLLGSQLTLAEGRVHLGEPATGLPGAVRTAVAEVR
                     AELARQPFAAPEARRLAELGLGAKQLAAAVRAGELLKIVDGIYLAPGADDDAIERLRG
                     LPSPFTLSQARQALGTTRRVAVPLMEHLARRGITRQLPDGTHELCS"
     gene            complement(453..>1542)
                     /locus_tag="VHH53_05555"
     CDS             complement(453..>1542)
                     /locus_tag="VHH53_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289654.1"
                     /translation="LLGLAATVYVVTTALLVPDDGTNPLPYAVYVLFWVGLVPLSLLF
                     GPVWRLVNPLRTIHLLLAAITRIPAERGLLPLPQWLGYWPAALSLLSFVWLELAAPNG
                     AAVNVLLLYFTVYAAVHLSAALLFGQRWFATGDGFEAYSTLVGTMAPFGRRDDGRLVL
                     RNPLDGVATFGPAPGIVALIAVLLGSTFFDSVSGSPDWTRAIQAAPVPAVLISTAGLL
                     LMIATVALTFVGATVLAGRAGHRGRKPVPGLLAHTVIPIVVGYVIAHYFSLLLFQGQQ
                     AIVMLSDPLGTDAGISGAVNYGLVTATGIAFIQVIAVVIGHVLAVISAHDRSVALFPQ
                     SEAVVGQLPLMVLMVGYTVGGLTLLFAA"
BASE COUNT          300 a          532 c          519 g          191 t
ORIGIN      
        1 ccggaatgcc cgacgcgcgg ctggtcgaac tgctgctcgg ctcccagctg actctggccg
       61 aaggacgagt gcacctcggc gaaccggcca ccggtctgcc cggcgcggtt cgcaccgccg
      121 tcgcggaggt tcgcgccgag cttgcgcggc agccattcgc cgcgcccgaa gcgcgccggc
      181 tcgccgagct cgggctgggc gccaagcagc tcgccgccgc ggtacgcgcc ggcgaactgc
      241 tcaagatcgt cgacggaatc tacctcgccc ccggcgccga cgatgacgcc attgaacggc
      301 tgcgcggcct gcccagcccg ttcaccctca gccaggcccg ccaagccctc ggcaccaccc
      361 gccgcgtcgc cgtccccctg atggagcacc tagcccgtcg cggcatcacc cgccagctcc
      421 cggacggcac tcacgagctg tgctcgtgag tcttacgctg cgaaaagcag cgtaagcccg
      481 cccacggtgt acccgaccat caggaccatc agcgggagct gcccgacgac ggcttccgac
      541 tgggggaaca gtgcaaccga acggtcatgg gcgctgatga ccgcgaggac gtgtccgatg
      601 acgactgcga tcacctggat aaaagcgata ccggtcgccg tgacgagccc gtagttgacg
      661 gcaccggaga tgcccgcatc ggtcccgagt ggatcggaga gcatgacgat ggcttgctgt
      721 ccctgaaaga gcagcaaaga gaagtagtga gcgatgacat aacccacgac gatggggatg
      781 acggtgtgcg ccagcagccc cggcaccggc tttcggccgc ggtgaccggc acgaccggcc
      841 agcacggtcg ccccgacgaa cgtcaaagcc acagtggcga tcatcaggag cagtccggcc
      901 gtggagatca gcaccgccgg aaccggcgct gcttggatgg cgcgggtcca gtccggcgaa
      961 ccggagacgc tgtcgaagaa ggtagagccc agcagcaccg cgatcaaagc gacgatgccc
     1021 ggtgcaggtc caaacgttgc caccccgtcc agcggattgc gaagcacgag acgaccgtca
     1081 tcacgccggc cgaaaggcgc catcgtgccg accaacgtgg agtaggcctc gaacccgtcg
     1141 ccggtggcga accagcgctg gccgaacagc agcgccgcgc tgagatggac ggcagcgtag
     1201 acggtgaaat acagcagcag cacgttgact gcggcgccgt tcggcgcggc cagctccagc
     1261 cacacgaagc tgagcagcga cagtgccgcc ggccagtagc ccagccattg cggtagcggc
     1321 agcagtcccc gttcggcggg aatccgggtg atggcggcga gcagcaggtg aatcgttcgc
     1381 agcgggttga ccagccgcca caccgggccg aacagcagcg acagcggaac gagccctacc
     1441 cagaacagca cgtacaccgc gtagggcagc gggttggtgc cgtcgtcggg taccagcagc
     1501 gccgtggtga cgacgtagac cgttgcggcg agaccgagca gg
//
LOCUS       DATADN010000424         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_35023, whole genome shotgun sequence.
ACCESSION   DATADN010000424 DATADN010000000
VERSION     DATADN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35023"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VHH53_05560"
     CDS             complement(<1..250)
                     /locus_tag="VHH53_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2289655.1"
                     /translation="MPTGRVKWYDAEKGFGFLSQEDGEDVYVRSSALPSGVETLKAGQ
                     RVEFGIAAGRRGPQALTLKVIDPPPSLSRTRREAAAAER"
     gene            418..834
                     /locus_tag="VHH53_05565"
     CDS             418..834
                     /locus_tag="VHH53_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX2289656.1"
                     /translation="MRLILNVIWLVFGGLWLALGYLLAALICFVLIITIPFGFAALRI
                     AVFALWPFGNTVVDKPGPRPGALIGNVIWIILFGIWLAIGHVITAAAMAITIIGIPLA
                     LANLKLIPVSLMPLGKDIVPVDSWQGPSYNPQRVAA"
     gene            840..1880
                     /gene="moaA"
                     /locus_tag="VHH53_05570"
     CDS             840..1880
                     /gene="moaA"
                     /locus_tag="VHH53_05570"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX2289657.1"
                     /translation="MDLGVPSAAPSSPAPSDGPLVDTFGRVATDLRVSLTDRCNLRCT
                     YCMPADGLDWLPSDRLLRPEELTRLLRIAVTRLGITSVRFTGGEPLVAKHLEDVIAAT
                     AALRPRPEITLTTNGIGLAKRADGLKRAGLDRINVSLDTVDSARFAAITRRDRLSDVL
                     AGLRAAKAAGLDPVKVNAVLDPVTGLDDAVALLRFCLEHDYQLRIIEQMPLDAGHEWQ
                     RGKAIGADQVLAALQQHFHLTPDTAPRGSAPAELWRVDGGAGVVGIIASVSHAFCAAC
                     DRTRLTADGQVRNCLFATEESDLRTALRDGADDGELARRWRLAMWGKRAGHGIDDPSF
                     LQPARPMSAIGG"
BASE COUNT          295 a          720 c          661 g          321 t
ORIGIN      
        1 tgcgttcggc ggccgccgcc tcccgacgcg tacgcgacag gctgggcggc ggatcgatga
       61 ctttcagcgt caacgcctgc ggcccgcgac gccccgcagc gatgccgaac tcgacgcgct
      121 gcccggcctt gagcgtctcg acaccagagg gcagcgcaga ggaccggacg tagacgtcct
      181 caccgtcctc ctgcgacaga aagccgaagc ctttctcggc gtcgtaccac ttaaccctgc
      241 cggtcggcac tggtctcacc cgcttgtctt gacgtcaaca taggaaacat aggaaacaca
      301 cgaaagcgcc ccgaatgcgc cgggacgcgt gttgcgatcc tactctgacc ccaacctcgc
      361 cagcaccccc ttatcggtag gcttggagcc accgctgaag gagaagaagc gcccgctatg
      421 cgattgatcc tgaacgtcat ctggttggtc ttcggcggcc tgtggctggc gttgggctac
      481 ctgctggccg cgctgatctg cttcgtgctg atcatcacga ttccgttcgg gttcgcagcc
      541 ctgcgcatcg cggttttcgc gctgtggccg ttcggcaaca ccgtcgtcga caaacccggg
      601 ccgcggcccg gcgcgctcat cggcaacgtc atctggatca tcctgttcgg catctggctg
      661 gcgatcggcc atgtcatcac cgccgcggcg atggcgatca cgatcatcgg catcccgctg
      721 gcgctggcca acctgaagct gattccggtg tcgctgatgc cgttgggcaa ggacatcgtg
      781 ccggtcgaca gctggcaagg ccccagctac aacccgcagc gagttgccgc gtgacagtca
      841 tcgacctcgg tgtgccgtcg gcggcaccat cctcaccggc ccccagcgac gggccgctgg
      901 tcgacacgtt cggccgggtg gccacggatc tgcgggtgtc gctgaccgac cgctgcaacc
      961 tgcgctgcac ctactgcatg cccgccgacg gcctggattg gctgccctcc gaccgcctgc
     1021 tgcggcccga ggaattgacc cggctgctgc gcatcgccgt gacgaggctc ggcatcacca
     1081 gcgtgcgctt caccgggggg gagccgctgg tcgccaagca cctcgaagat gtcattgccg
     1141 cgacggcggc gctgcggcca cggccggaaa tcacgttgac caccaacggt atcggcctcg
     1201 ccaagcgggc cgacgggctc aagcgggcag ggctggaccg catcaacgtg tcgctcgaca
     1261 ccgtcgactc cgcgcggttc gccgccatca cccgtcgtga ccggctctcc gatgtgctgg
     1321 cgggcctgcg cgcggccaag gccgcgggtc tcgacccggt gaaggtcaat gcggttcttg
     1381 accctgtgac cggtctcgac gacgcggtcg cgctgctgcg gttctgcctc gagcacgact
     1441 accagctgcg catcatcgag cagatgccgc tggacgcggg tcacgagtgg cagcgcggca
     1501 aagcgatcgg cgccgaccag gtgcttgccg cgctgcagca gcatttccac ctcaccccgg
     1561 acaccgcccc gcgcggttcg gcaccggccg agttgtggcg cgtcgacggc ggggcaggcg
     1621 tggtcggcat catcgcctcg gtttcccacg cgttctgcgc cgcctgcgac cgcacccggc
     1681 tgaccgccga cggccaggtg cgcaactgcc tgttcgccac cgaggagtcc gacctgcgca
     1741 cggccctgcg cgacggcgcg gacgacggcg agctggcccg gcgctggcgg ctggcgatgt
     1801 ggggcaagcg ggccggccac ggcatcgatg acccgtcgtt cctgcagccc gcccggccga
     1861 tgtccgcgat cgggggttga ggtggccggc gagctgaccg tccgctactt cgccggcgcc
     1921 cgcgccgccg ccggaacgac cgaggagtcg gcaccggccg acctggacct ggacgcgctg
     1981 acggacctgc tcgccgg
//
LOCUS       DATADN010000425         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_35454, whole genome shotgun sequence.
ACCESSION   DATADN010000425 DATADN010000000
VERSION     DATADN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35454"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VHH53_05575"
     CDS             complement(<1..1163)
                     /locus_tag="VHH53_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX2289658.1"
                     /translation="MDSPPLLPALHQPTDAEALRFGQHSVTHRELSVVAAALQAPLAG
                     ARRVAVWAAPQLQTCVAIVAALAAGVPAVPINPKLGTRELQHVVSDARPDMVLTAPGA
                     TLPAALRALPRLDVDLRSRGAPMPPEPDPESPALIIYTSGTTGPPKGVVLPRRALAAD
                     VDALASAWRWTAQDVLVHGLPLFHVHGLVLGVIGPLRLGGAVHHLGSFSVQGTARELA
                     GCALDDRATMLFGVPTMYHRLAAAAEQESQVAAALGRARLLVSGSAALPVSDHQRIAA
                     VTGQHVVQRYGMTESLITCAVRADGQRRPGTVGPPLDAVGLRLVDERGSVLQVTDDET
                     VGEIEVAGPTLFLGYLNRPDATAEAMHDGWFRTGDLATWDRDGYLRLVGRRVT"
     gene            1207..2364
                     /locus_tag="VHH53_05580"
     CDS             1207..2364
                     /locus_tag="VHH53_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289659.1"
                     /translation="MAQRAGIVDDQPRPFEWWMTSHLALALAFNCFLPVLLPAYVLSV
                     GGSAIDIALAMSMAGLFALIGPSIGGLAVRHRAHRLVQALGVLGMAIGLGALAVSGGD
                     SLSIVLAIAVMGVGAAAVAVAAPTLILGTDLPPHIQERQLTWLQLNLDLGKIFGGLVL
                     GAMAAAKLPFHTQFRVGGLAIGLLSVLVWTSSRGAATRICQPQAHPTEADEPSRTVVP
                     LKTLLLLSLFGVPVVIHLVGSMTMMEAQISTMLTVSGLVGLGLYFLTGRWMVRSSPGM
                     IWAVGHTLRGVRGIALGVFGAVRGMPYLVVVATFLVMESIPAVVWIGQARTAAQFTPK
                     VTAMVTEAAKASRRSVPAGRLDQQRGRHAESLGVFHDLASPSVVRSQPAVQ"
BASE COUNT          381 a          815 c          802 g          415 t
ORIGIN      
        1 tccgtgaccc ggcgtccgac caaccgcagg tagccgtccc gatcccaggt ggctaggtcg
       61 ccggtacgaa accagccatc gtgcatcgcc tcggcagtag cgtcgggtcg gttgagataa
      121 cccaggaaca acgtcggccc ggcaacctcg atctctccga cggtctcgtc gtcggtcacc
      181 tgcaggaccg aaccccgctc atccaccagc cgcagcccca cggcatccag cggcggcccg
      241 acggtgcccg gccggcgttg cccgtcagcc cgcaccgcgc aggtgatcag cgactcggtc
      301 atcccgtacc gctgcaccac gtgctgaccg gtcaccgcgg cgatgcgctg gtgatcggac
      361 acgggcagtg cagcagaacc cgagacgagc agccgggccc gcccaagagc tgcggcgacc
      421 tgggattcct gctcagctgc ggcggccaac cggtggtaca tcgtcggcac gccgaacaac
      481 atcgttgccc gatcatccaa tgcacagccg gccaattccc gggccgttcc ctgcaccgag
      541 aatgagccca ggtggtgcac ggcgcccccc agtcgcagcg gcccgatgac gcccagtacc
      601 aggccgtgca cgtgaaacag cggcagaccg tgcaccagga cgtcctgggc agtccaccgc
      661 caggctgacg cgagcgcgtc gacatcagcg gcaagcgcgc gccgcggcag caccacgccc
      721 ttgggcggac cggtggtgcc cgaggtatag atgatcagtg ccggtgactc gggatcgggc
      781 tcgggcggca tcggagcacc ccgcgaccgc agatcgacgt cgagccgcgg cagggcacgc
      841 agggccgcgg gcaaggtggc tcccggggcg gtgagcacca tgtcgggtct cgcatcggag
      901 acgacatgct gcagctcccg ggtgcccagc ttgggattga tcggcaccgc aggcaccccc
      961 gcggccagcg ccgccacaat cgccacgcag gtctgcagct gcggcgcggc ccagaccgcc
     1021 acgcgccggg caccggcgag cggggcttgc aaagccgcgg ccaccacact cagttcccga
     1081 tgtgtcaccg aatgctgacc gaatcgcaac gcctcagcgt cagtgggttg gtgcaatgcc
     1141 ggcaacaatg gcggactatc cacttgcaat cctttcaagg tacttgttga ggtaatgtct
     1201 gccaccatgg ctcagcgtgc tggcatcgtg gatgatcagc cccggccttt cgaatggtgg
     1261 atgacctcgc accttgccct ggcattggcc ttcaactgct tcctacccgt tctgctgccg
     1321 gcgtacgtgc tctcggtcgg cgggtccgcc atcgatattg cgctcgcgat gtcgatggcc
     1381 ggcctcttcg ccttgatcgg gccctcgatc ggggggctcg cggtgcgcca ccgggcccac
     1441 cggctggtgc aggcgctggg tgtgctgggg atggccatcg gtctcggggc attggccgtg
     1501 tccggcggtg attcgttatc gatcgtgctc gccatagcgg tgatgggtgt cggtgcggcg
     1561 gcggtggcag tcgcggctcc aaccctcatc ctgggcaccg atctgccccc gcacatccag
     1621 gaacggcagt tgacctggct gcagctcaac ctggatctgg gcaagatctt cggggggctg
     1681 gtactcggtg cgatggcagc tgcgaaactg ccgttccaca cccagttccg ggttggcggg
     1741 ctggcgatcg gcctgctcag cgtgctcgtg tggaccagca gtcgcggcgc ggccacccgg
     1801 atctgccagc cccaggctca tcccaccgaa gcggacgaac cctcgcgcac cgtggtaccc
     1861 ctgaagacac tcctgctgct gtcgctgttc ggcgtcccgg tggtgatcca cctagtgggc
     1921 agcatgacca tgatggaggc gcagatctcc acgatgctga ccgtgtccgg tctggtaggc
     1981 ctcgggctgt actttctgac cggtcgctgg atggtccggt ccagccctgg gatgatctgg
     2041 gcggtcggcc acacgctgcg cggagtgcga ggcatcgcgc tgggcgtatt cggtgctgtg
     2101 cgtgggatgc cctacctcgt cgtggtagcc acattcctgg tgatggaatc gattcccgcg
     2161 gtggtgtgga ttggccaggc acgaacggca gcacagttca ctcccaaggt gaccgcgatg
     2221 gttactgagg cggcgaaggc ctctcgacgc agtgtgcccg ccggacggct ggaccagcag
     2281 cgaggccgcc acgccgagtc cctcggagtg tttcatgatt tggcctcgcc aagcgttgtc
     2341 cggtcacaac ctgctgtgca gtgaaacacg gcgcgtgatt cttcagcttt accggtgagt
     2401 cgtttcggac tgg
//
LOCUS       DATADN010000426         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_654517, whole genome shotgun sequence.
ACCESSION   DATADN010000426 DATADN010000000
VERSION     DATADN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654517"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1967
                     /locus_tag="VHH53_05585"
     CDS             <1..>1967
                     /locus_tag="VHH53_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289660.1"
                     /translation="PRPARAPRRDGSRAGRAHRLYLRRTIDLYAADLAGWRLAHCTLP
                     GLRAANLRVRSELALEDCAVTGPIVLSDARIEGPLRLIGTRLDIAGDHALAGVRLIIR
                     GVLDARELQADGEVRLSGARIDGNIDLRGARLSHPSGDALEAAGVQVGGNLRCDRAFS
                     AQGRVVLAGASVAGDAVFSGATLRGTTDPAERAVLVLPRGSADSTAALVADRIAVHGN
                     LVLDAGFTAVGSIRVTNARIGGYLQLSGATLGQPVEPDTANGDHSQPVPVALAADGIE
                     VHGDLEARQAPTGAGDFGALQAYGQVRLVGAQVNGSASLSGVRLHGPGLDVLFADRLR
                     IGGSLFMRRLVAAGSIRLHHAHIGSTLNCTAARLDAPRRRTDGSIKPSLDARAATIGK
                     DIYASRGFTATGGVRLSLAEVSKSVNFTNARLGGAGEGAAALHARGMTCQELRLGFIE
                     PPRGDVSLAGVVAGSVVDSAQLWATDGKVDVEDFRYQSLTASPDVDVATRLRWLRAVL
                     LEYDPDPYDSLAASYRDCGHDDRADAVLLAKQQHRHTVHGPAGRFWGWLQEWTVGYGY
                     RPWRAALWLAVCWLLGSLWFAYHQLPRLDSGQDPSWQPVIYAADLLIPVVNLGQDGLW
                     RTSGGSAWVAGVLTAVGWLLLSTGAAGVTRIL"
BASE COUNT          271 a          643 c          724 g          329 t
ORIGIN      
        1 ccgcggcccg ctcgagctcc ccggcgcgac ggttcccgcg ctggtcgagc tcaccggctg
       61 taccttcgac gaaccatcga cctgtacgcc gcggatctcg ccggttggcg tcttgcgcac
      121 tgcacactgc ccggcctacg ggcggccaac ctgcgggtac gcagcgagct ggcgctggag
      181 gactgcgcag taaccgggcc gattgtgctg tccgacgccc ggatcgaggg accgttgcgg
      241 ctgatcggca cgcgcctgga catcgcgggt gatcacgctc tggccggagt aaggctgatc
      301 atccgcggcg tgctcgacgc ccgggagctg caggctgacg gtgaggtgcg cctgtccggt
      361 gcgcggatag acggcaacat cgatcttcgc ggcgcgcggc tgtcccatcc cagcggtgac
      421 gcgctggaag ccgcgggggt gcaggtcggc ggcaatctgc gctgtgatcg ggcgttcagc
      481 gcgcagggtc gggtggtgct ggccggtgcc tcggtcgcag gggacgcggt cttctccggt
      541 gcaacgctgc gaggaaccac ggacccagcc gagcgtgctg tgctcgtgct gccgcgcggt
      601 agtgccgatt ccaccgcagc gctggtggcc gatcgcatcg cggtgcacgg caacctggtg
      661 ctcgacgcgg ggttcaccgc cgtcggcagc atccgggtga cgaacgctcg aatcgggggt
      721 tacctgcaac tttccggtgc gaccttgggc caaccggtcg agcctgacac cgccaacggt
      781 gatcactcgc agcccgtgcc agtggcactg gcagccgacg gtatcgaagt gcacggggac
      841 ctggaagcgc gccaagcacc caccggagca ggtgacttcg gtgccctaca ggcgtatggg
      901 caggtgcggt tggtcggtgc gcaggtgaac ggcagcgcga gtctgtccgg tgtgcggttg
      961 cacggtccgg gcctggacgt gctgttcgca gaccggctgc ggatcggcgg atcgctgttc
     1021 atgcgaaggc tggtggccgc agggtccatc cggctgcatc atgcccacat cggctcgacg
     1081 ttgaactgca ccgctgcccg gctcgacgcg ccacggcgcc gtacggacgg ctccatcaag
     1141 ccgtcgctgg atgcccgggc cgccaccatc ggtaaggaca tctacgccag ccgcgggttc
     1201 accgccaccg ggggtgtgcg gctcagcctt gctgaggtgt cgaagtcggt gaacttcacc
     1261 aacgcgcggc tcggcggggc cggcgagggg gcggcggcgc tgcacgcccg cgggatgacc
     1321 tgccaggaac tgaggctggg attcatcgag ccaccgcgcg gtgacgtcag cctcgccggt
     1381 gtggtggccg gctccgtcgt cgacagtgcg cagctgtggg ccaccgacgg caaggtggac
     1441 gtggaggact tccggtacca gtcgctcacc gcctcgcccg atgtcgacgt ggccacccgg
     1501 ctgcgctggt tgcgggcggt gcttctcgaa tacgaccctg atccttacga cagcctggcg
     1561 gcaagctacc gcgactgcgg ccatgatgat cgcgcagacg cggtgctgct ggccaagcag
     1621 caacaccggc acactgtgca cggaccggcc gggcggttct ggggatggct tcaggagtgg
     1681 accgtcggct acggctaccg gccctggcgg gcggcgctgt ggcttgccgt gtgctggctg
     1741 ctcgggtcgc tgtggtttgc ctaccaccag ctaccccggc tcgacagcgg tcaggatccg
     1801 tcgtggcaac cggtgatcta tgccgcagac ctgctgatcc cggtggtgaa cctgggacag
     1861 gacgggctat ggcggacctc tggcggctcg gcctgggttg ccggcgtgct gaccgcggtt
     1921 ggttggttgc tgctgtccac cggagcggcc ggggtgaccc ggatcct
//
LOCUS       DATADN010000427         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_191694, whole genome shotgun sequence.
ACCESSION   DATADN010000427 DATADN010000000
VERSION     DATADN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191694"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1416
                     /locus_tag="VHH53_05590"
     CDS             91..1416
                     /locus_tag="VHH53_05590"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2289661.1"
                     /translation="MTDLDRLLPASEAARLDAYLDRLVLVLRQPVRGRMARAYCLGLL
                     AAPGRKSIEPLAGLHGARHGSGLYQSLHHFVADARWDDAALLAAVAREVLPAIERHGP
                     IRAWIVDDTALAKKGRHSVGVAPQYSGRLGTTANCQVAVSLSLANAAASLPVAWQLYL
                     PEAWAADHERCRAAGVPLAVRFRTKLEIALEQLGAAVVLGLPKGVVLADEAYGGAVQF
                     RQGTRALGLDYAVALDRAQGLLLPDGKGPLAARAAAHLLPEAAWQAMPGRPAGLRYAA
                     LRVRAAPYGRPGPEEWLLCERGQGKAAEERFWLTSLPAETGLEELVTIATLRWRVERD
                     YEELKQEVGLGHFQGRGWRGFHHHASLCIAAYGFLVQERCLFPPESQGWPDRAGDPGT
                     AAAAQPLLHSLAQAGAPAGPDPTPALLPLVRHDPNLIPQRARQPASVIQ"
     gene            1479..>1676
                     /locus_tag="VHH53_05595"
     CDS             1479..>1676
                     /locus_tag="VHH53_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289662.1"
                     /translation="MPALATASYATRRGLTRPFARQTAQHRRLGQKLSMGLSAAQAAR
                     LDGLGCQALEAGGLGWGEADRE"
BASE COUNT          233 a          586 c          584 g          273 t
ORIGIN      
        1 gcttcgaggc agacatgact tcctcccaac gcccgagagg atagcatgcc tgttgggtac
       61 gtcaacgtca caatcgccta tatagtgggg atgactgact tggatcgcct gctgccggcg
      121 tctgaggctg cgcgcttgga cgcctatctg gaccggctcg tgctggtctt gcgccagccg
      181 gtgcgggggc ggatggcgcg ggcctactgc ctgggcctgc tggcagcacc tgggcgcaag
      241 agcattgagc ctctggccgg gctgcatggc gcgcggcacg gatcggggct ctaccagtcc
      301 ctgcaccact ttgtcgccga tgctcgctgg gacgacgcgg cgctgctggc cgcggtcgcg
      361 cgcgaggtgc tgccggcgat cgagcggcac gggccgatcc gggcctggat cgtcgatgac
      421 accgcgcttg ccaagaaggg gcggcactcg gtgggcgtag caccccaata ttcgggccgg
      481 cttggcacga ccgccaactg ccaggtggcg gtcagcctct ccttggccaa tgccgccgcc
      541 agcctgccgg tggcctggca gctctacctg cccgaggcct gggcggccga ccacgagcga
      601 tgccgtgccg ctggggtacc gttggcggtg cggttccgga ccaagctcga gatcgccctg
      661 gagcaactgg gcgctgcggt cgtgctgggc ctgcccaagg gcgtggtact ggcggacgag
      721 gcttatggcg gtgccgttca gttccgccag ggcacacggg cactcggtct cgactatgcg
      781 gtcgcgctcg accgcgcgca ggggctgctc ctccctgacg gcaagggccc gctcgcagcc
      841 agggcggcgg cacaccttct gcccgaggcg gcatggcagg ccatgccggg ccgcccggcc
      901 ggcctgcgct acgcggccct gcgggtgcgg gcagcccctt atggccggcc cgggcccgag
      961 gagtggctgc tgtgcgagcg tggccagggc aaggctgccg aggagcgctt ctggctcacg
     1021 agcctgccgg ccgagactgg ccttgaggag cttgtcacca tcgccaccct gcgctggcgg
     1081 gtcgagcgcg actatgaaga gctcaagcag gaggtgggct tgggtcattt ccagggccgc
     1141 ggctggcgcg gcttccacca ccacgcctcc ttgtgcatcg cggcttacgg cttcctggtc
     1201 caggaacgct gcctttttcc ccctgaatca caaggctggc ccgatcgtgc cggcgacccc
     1261 ggcacggccg cagcggcaca acccctactc catagcctcg ctcaagctgg cgctcctgca
     1321 gggcctgatc cgacgcctgc cctgctgccc ctggtgcggc acgatcccaa cctcatcccc
     1381 caacgcgcgc gacagcccgc cagcgtgata cagtagggcc agtgatctag aggataggat
     1441 ccttgtaagc aggtataaat tcctgtaatg ttcgctctat gcctgcgctc gccaccgcgt
     1501 cctacgccac ccgccgcggc ctcacgcgcc cgttcgcaag gcagacggcc cagcaccgcc
     1561 gcctgggcca gaagctctcg atgggcttga gcgccgccca ggccgcccgc ctcgacggcc
     1621 tggggtgcca ggccctcgag gcgggcggcc tgggctgggg tgaggccgat cgagag
//
LOCUS       DATADN010000428         2285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_35656, whole genome shotgun sequence.
ACCESSION   DATADN010000428 DATADN010000000
VERSION     DATADN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35656"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>2285
                     /locus_tag="VHH53_05600"
     CDS             18..>2285
                     /locus_tag="VHH53_05600"
                     /inference="COORDINATES: protein motif:HMM:NF037374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HEX2289663.1"
                     /translation="MISIAALAAGLAVKADNAEAQTAPTIQSNLDDYPPGGLVTLTGS
                     NWQPGETVNIYVNDDVGKTWERNVNVTADPSGNITDQFNLPDWFVAVYQVRATGAQSG
                     VATTSFTDGDVRFAGSANAPATWKQNYFTYSSRDCTGTGTAQQLTMSGAAEQRLTLTG
                     TQSLKLNGVDESSTQVTFDRWTRDDKDVTSPVTTADCLSGQGAEHRITTHFKAVQNQT
                     ISFVKPPDKTYGDAPFQVSATASSGLPVSMVAEGQCTLAAGSTSGTWTVNITGAGNCT
                     LTATQAGNDAYNAALPVAHTFAIAKAGQTIAFNAGTPTTKTYGDAPFQVGATASSGLP
                     VSYAAKAGSACTVSTSGLVSITGAGSCTIVASQQTGDANYFPAPNKEHTITVNPKVVT
                     GSFTANDKVYDGNNSATVKSTALPGVLAGDVVNLVVTNAQFADKNVSTTAKTVRADLA
                     LSGADAGKYTLSSTVGTTTANITPKSLTGSFTANNKVYDGNTDATIATSNLDGRITGD
                     VVTLSGDTATFDNRNVGTGKTVTVNRAALSGADAGNYMLAQGPWTTTADITAKELTGS
                     FTAANKSYDGNTSATIATRSVGGEVSGDDVSLVGGTATFDNANVGTAKTVTATGFSLS
                     GAAEGNYILRAGPWTTTADINRKELTGSFTAANKTYDGNTSATIATRSLQDVVGDEAV
                     SLTGGTATFADKNVGQGKTVTATGFTLTGTDANNYTLKAGPWTATANITPKSLTGSFT
                     ANNKVYDGNTDATIAT"
BASE COUNT          500 a          777 c          669 g          339 t
ORIGIN      
        1 ttttggcgtt tgctgtaatg atctcgatcg cggcgctcgc ggcggggttg gccgttaagg
       61 cggacaacgc ggaggcccaa actgccccta ccatccagag caacctggac gactacccgc
      121 cgggtgggct cgtgaccctc accgggtcca actggcagcc cggcgagacc gtcaacatct
      181 acgtcaatga cgatgtaggc aagacttggg agagaaacgt caacgtaacc gccgacccaa
      241 gcggcaacat cacggatcag ttcaacctac cggactggtt cgtggccgtt taccaggtga
      301 gggccacggg cgctcagtcc ggcgtggcga cgacctcctt taccgacggg gacgttaggt
      361 ttgctggcag cgcgaacgcg cctgcgacct ggaagcagaa ctattttacg tatagctcca
      421 gagactgcac cggaacgggc actgcccaac agttgacgat gtccggcgct gcggagcagc
      481 gactaaccct cactgggacc cagagcttga aacttaacgg tgtggacgaa agctccactc
      541 aagtcacttt cgacaggtgg accagagacg acaaggatgt cacctccccc gtgactactg
      601 cagactgttt gtcgggtcag ggagcggagc accgtatcac cacgcacttc aaggctgttc
      661 agaaccagac gattagcttc gtcaagccgc ccgataagac ctacggcgat gctccgttcc
      721 aggtgagcgc cacggccagc tcggggctgc ctgtcagcat ggtcgccgaa ggccagtgta
      781 cgctcgctgc gggctctact agtggcacct ggactgtgaa catcaccggg gcgggtaact
      841 gcaccctgac cgccacgcag gccggcaacg atgcctacaa cgccgcgctc cctgtggcgc
      901 atactttcgc gatcgccaag gcgggccaga cgattgcctt caatgcgggt acgccgacga
      961 cgaagaccta cggcgatgct cccttccagg tgggcgccac ggccagctcg gggctcccgg
     1021 tcagctacgc ggccaaggca ggcagcgcgt gtacggtgag caccagcggc ctcgtgagca
     1081 tcactggagc gggtagctgc accatcgtcg cctcgcagca gaccggcgac gccaactact
     1141 tcccagcgcc taacaaagag cacaccatca ccgtcaaccc caaggtggta acgggcagct
     1201 tcacggccaa cgataaggta tacgacggca acaactccgc aaccgtgaag agcaccgcgc
     1261 tgccgggcgt gctcgctggt gatgtggtca accttgtcgt caccaacgct cagttcgccg
     1321 acaagaacgt gagcaccacc gccaagacgg tgagggcgga cctggctctc tcgggtgccg
     1381 acgccggcaa gtacaccctg agctcgacgg tgggcactac cactgccaac atcacgccga
     1441 agtccctcac cggtagcttc actgccaaca acaaggtcta cgacggaaac accgacgcca
     1501 cgatcgccac cagtaacctc gacggtagga tcaccggcga cgtcgtcacc ctgtccggcg
     1561 acacggccac gttcgacaac agaaacgtcg gcaccggcaa gaccgttacc gtcaacaggg
     1621 ccgccctctc cggcgccgat gccggcaact acatgctggc gcagggtccg tggaccacca
     1681 cggccgacat cacggccaag gaactgaccg gcagcttcac ggcggccaac aagagctacg
     1741 acggcaacac ctccgccacc atcgccaccc gcagtgttgg tggtgaggtg tccggtgatg
     1801 acgtcagcct cgtcggcggg acggccacgt tcgacaacgc aaacgtgggc accgccaaga
     1861 cggtgaccgc cacgggcttt agcctctccg gggccgccga gggcaactac atcctgagag
     1921 ccggtccgtg gacgaccacg gctgacataa accgcaagga actcaccggc agcttcacgg
     1981 cggccaacaa gacctacgac ggcaacacct ccgctaccat tgccacccgc tcgctgcagg
     2041 acgtggtcgg cgacgaagcc gtcagcctca cgggcggcac ggcgaccttc gccgacaaga
     2101 acgtcggcca gggcaagacg gtgaccgcga ccggcttcac gctcaccggc acggatgcta
     2161 acaattacac cttgaaggct ggtccgtgga cggccactgc caacatcacg ccgaagtccc
     2221 tcacgggcag cttcaccgcc aacaacaagg tctacgatgg caacaccgac gccacgatcg
     2281 ccacc
//
LOCUS       DATADN010000429         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_507651, whole genome shotgun sequence.
ACCESSION   DATADN010000429 DATADN010000000
VERSION     DATADN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507651"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..>1501
                     /locus_tag="VHH53_05605"
     CDS             210..>1501
                     /locus_tag="VHH53_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2289664.1"
                     /translation="MATPTPLPDTDPSAAPSTKRGLELIMLAFASVVVTTALVLVELN
                     QQQEVTRSLLYYGAAYLALFGAAHIAVRLLAPYADPLILPCVALLNGLGLVLIHRLDL
                     AEATAAVSVGDPIPDGNVPHQIAWTALALGLFVAVLVLIHDHRMLARYGYTCGLVGLV
                     LLALPGLLPSAISEVNGAKIWLRFGGLGIQPGEFAKILLMIFVAAFLVAKRDLFTTAG
                     RSVLGMEFPRARDLAPLLMAWGLSLGVLVLEKDLGTSLLYFGIVLVLIYIATERAAWM
                     VIGLIFFVVGAMVSGMLFTHVQRRVTAWLDPFSDYDGAGYQMAQSLFGLGTGGVFGTG
                     LGAGRPDLVPAAHTDFITAAIGEELGFVGLAAVLFTYLLLVMRGMRTALTVRDTFGKL
                     LAAGLSFAVGLQVFVVVGGVTKLIPLTGLTAPFLSYGGS"
BASE COUNT          199 a          515 c          485 g          302 t
ORIGIN      
        1 gcactaatgg cctgctgcca ccatgccccc ggaccggcat tggggcaacc accgttgagc
       61 aaccgtcccc gcccaccccc tcgagttcgc cgagcccatc tcttggcggg cagctgtccg
      121 gccagcaccc cgaacccgcg atcaccggca cgccgattcc gcagacaccc tcggaacccg
      181 gagtggactg ccggacggtg agctagctga tggccacccc aacacctctc ccggacacgg
      241 acccatcggc cgcgccgtcc accaagcgcg gcctcgagct gatcatgttg gccttcgcct
      301 cggtcgtggt gaccaccgcg ctggtgttgg tggagctcaa tcaacaacag gaggtaaccc
      361 gttcactgct ctactacggc gccgcctacc tagctctgtt cggcgccgcc catatcgcgg
      421 tgcggttgct ggctccgtat gccgacccgc tgatcctgcc ctgcgtcgcg ttgctcaacg
      481 gtctgggcct ggtgctcatc caccggctcg atctggcgga ggcgactgcg gccgtatcgg
      541 tcggcgaccc gatcccggat ggcaacgttc ctcatcagat cgcctggacc gcgctcgcct
      601 tggggctgtt cgtggcggtt ctggtactga tccacgacca tcgcatgctg gctcgctacg
      661 gctacacctg cgggctggtc gggctggtgc tgctggccct gcccggcttg ctacccagcg
      721 ccatctccga agtcaacggc gcgaagatct ggctacggtt cggcgggttg ggtattcaac
      781 cgggcgagtt cgccaagatc ctgctgatga tcttcgttgc cgctttcctg gtcgccaaga
      841 gggacctttt caccactgcg ggccgcagcg tgctcggtat ggagttcccg agggcccgag
      901 acctcgcccc gctgctgatg gcgtggggac tctcgttggg tgtcctggtg ctcgagaagg
      961 acctgggaac ctcgctgctg tacttcggca tcgtgctggt actgatctac atcgccaccg
     1021 aacgggccgc ctggatggtg atcgggctga tcttcttcgt cgttggcgcg atggtttcgg
     1081 ggatgctctt cacgcacgtg cagcgccggg tgaccgcgtg gctcgatccg ttctcggact
     1141 acgacggcgc cggctatcag atggcacagt cactgttcgg gctgggtacc ggcggggtgt
     1201 tcggtaccgg cctgggtgcg ggccgtcctg acctggtgcc cgcggcgcac actgacttca
     1261 tcaccgcagc gatcggcgag gagctgggct tcgtcgggct ggccgccgtg ctgttcacct
     1321 acctgctgct ggtgatgcgg ggaatgcgca ccgcgctgac cgtccgggac accttcggca
     1381 agctgctcgc cgccgggctg tctttcgcgg tcggcctgca ggtgttcgtc gtcgtcggcg
     1441 gggtgaccaa gctgatcccg ctgaccgggc tcaccgcgcc gttcctgtcc tacggcgggt
     1501 c
//
LOCUS       DATADN010000430         4067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1114195, whole genome shotgun sequence.
ACCESSION   DATADN010000430 DATADN010000000
VERSION     DATADN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114195"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VHH53_05610"
     CDS             complement(<1..422)
                     /locus_tag="VHH53_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX2289665.1"
                     /translation="MHPAESLRRPPAAATALLDRLGQLDRLVVAFSGGVDSAVVLAAA
                     VRALGPDTVLAAIADSPSLARAEMATARRVAGEIGAELVALRTDEVADPGYRANAGDR
                     CYFCKHTVLAAVRDLAQRRGFAHVATGTHLDDRRAAHRP"
     gene            480..1217
                     /gene="larB"
                     /locus_tag="VHH53_05615"
     CDS             480..1217
                     /gene="larB"
                     /locus_tag="VHH53_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX2289666.1"
                     /translation="MVTPVPDAAQSQPSRPVTGDRIGEVDGTARLDLDRRRRTAVPEV
                     VFAQGKTEEQTLQLLAAQRRGDPGFPALATRCPAGVLQRVPARFPGETVVVDPVGRTV
                     IVGALPPARGLVAVLTGGTSDLPVAREAVSTLQALGVRHRLISDVGVAGLHRLLAQLH
                     ELSAADCVIAIAGMEGTLPGVVAGLIRAPVVAVPTSVGYGVSAGGHVAAAAMLASCAP
                     GLSVVNIDNGFGAAAHAVKIVARVHGG"
     gene            1210..2466
                     /gene="larC"
                     /locus_tag="VHH53_05620"
     CDS             1210..2466
                     /gene="larC"
                     /locus_tag="VHH53_05620"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX2289667.1"
                     /translation="MAELRAAYLDCTAGVAGDMLLGALLDAGADLAAVRAAVLSLGVS
                     DVDVHVERTRRCGLRCSLAVVTTPPGPAPIRSLPDVLAIIDAAHLDPAAARVATSAFR
                     LLADAEVEVHGIPPEQVHFHEVGAADALADVVGTAVAAEHLGLLADDAVVICSPLAAG
                     SGTAHTAHGTIPVPAPAVLQIVADRGMCLTGGDLAGERTTPTGAALTATLATPGLLPP
                     MTVDVVGCGAGHRDTAERPNITRLVIGSPVGQLTGPGPAEAVIVVETTVDDLNPELWP
                     GVLTAVRAAGAWDCWTTATVGRHGRPGQVLTALCTEAARPAVADALFRHSTTLGVRWA
                     SWQRSTLPRITVEVPVGPPGAEREIIIKVAAGPGGQRTAKPELADAERIAQELGWPVR
                     RVYEAALSAYHRAHPADAVAPRDNQS"
     gene            2564..>4067
                     /locus_tag="VHH53_05625"
     CDS             2564..>4067
                     /locus_tag="VHH53_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2289668.1"
                     /translation="MSADDELAGSHVLLTGATGFLGQATLERLLSSYPDTRVSVLIRP
                     RAGRCAADRLTALLRKNVFRSLGERLGEDGLAGVVRDRVTVLEGDLDSITLPTDLDVV
                     IHGASAVSFDPPVDEAFRTNVAGVAALYEELAAVGSSPHVVHVSTAYVAGTRKGLVVE
                     AALDHDVDWRLELEMALAARAEAERDSRRPEVLQRALAQAAEQHAKAGPQSTATDAER
                     LRTEWVTRRLVDYGRTRARSLGWPDVYTLTKALGERVAEQLWAQTGRPLSVVRPAIVE
                     SALRHPYPGWIDGFKMADPLIIAFGRGVLREFPGLPDSVLDVIPVDLVVNAILAAAAR
                     TPVTAPAYLHVSSGASNPLTFQNMYALCREYFDNNPMPVADRGHVDPPSWRFPGSQQV
                     ELMLRAGEQAAALAERVLLALPPSKRSQDWLTSLGRHQQDLVQLREYADLYGVYARAE
                     VIYDDRRLRELHASLPTQRAAEHGFDPTTIDWRAYLQDVHCPAITATVRRA"
BASE COUNT          607 a         1408 c         1438 g          614 t
ORIGIN      
        1 gggcggtgcg ccgcgcgccg gtcatcaaga tgggtgccgg tagcgacgtg ggcaaacccg
       61 cgccgctgcg ccagatcgcg cactgcagcg agcaccgtgt gcttgcagaa gtagcagcga
      121 tcaccggcat tggcccgata gccagggtcg gcgacctcat cggtgcgcag cgccaccagt
      181 tccgcaccga tctcgccggc gacgcgccgg gcggtcgcca tctcggcgcg cgccaggctg
      241 ggcgagtcag cgatggcggc cagcactgtg tcggggccca gcgcccggac ggccgcagcc
      301 agcaccacgg cggaatcgac gccaccggag aacgcaacca ccagccggtc cagctgcccc
      361 agccgatcga gcaatgccgt cgcggcggcc ggtggccgac gtagggattc ggcagggtgc
      421 atggtcgacg atcctcgtat ggtcgggcgg tcgggcaact acatgagata gtcggcttca
      481 tggtcacccc agtgcctgac gcagcgcaaa gccagccaag ccggccggtg accggtgacc
      541 ggatcggcga ggtcgacggt actgcgcgcc tggacctcga ccgcaggcgt cgcaccgccg
      601 tgccggaagt tgtcttcgcg caaggtaaga ccgaggagca gacgttgcag ctgctcgccg
      661 cgcagcgacg aggcgacccc gggttccccg ccctggccac ccgatgcccc gccggggtgc
      721 tgcagcgggt gcccgctcga tttccgggcg agaccgtggt ggtcgatccg gtcggacgca
      781 ccgtcattgt cggtgcgctg ccaccagccc gtgggcttgt cgcagtgctc accggcggca
      841 cctcggacct gccggtcgct cgggaggcag tcagcaccct ccaggcgctc ggcgtcaggc
      901 acagattgat cagcgacgtt ggggtagccg ggctgcaccg attgctcgcc cagctccacg
      961 agctgtccgc agccgactgc gtgatcgcca tcgccggcat ggagggtacg ctgcccgggg
     1021 tggtcgccgg gttgatccgg gcgccggtag tcgccgtacc caccagcgtc ggctacgggg
     1081 tgagcgcagg tgggcacgtc gccgcggcgg cgatgttggc ctcctgcgcc cctgggctgt
     1141 cggtcgtcaa catcgacaac gggttcggcg cggccgcaca cgccgtcaag atcgtcgcgc
     1201 gagtgcacgg tggctgaact gcgagccgcg tacctggact gcaccgcggg cgtggcaggc
     1261 gacatgctgc tcggcgcgct gctcgacgcc ggtgccgacc ttgctgcggt gcgcgctgcg
     1321 gtgctatccc tgggtgtgtc ggatgtggac gttcacgttg agcggacccg gcgctgcggg
     1381 ctgcgatgtt ccctcgcggt agtcacgaca ccacccggtc ctgcgcccat ccgaagtctg
     1441 cccgatgtac tggccatcat cgatgcggcg cacttggacc ccgctgctgc ccgggtggcc
     1501 accagcgcct tccggctgct tgccgatgcc gaagtcgaag tgcacggcat cccgcccgaa
     1561 caggtgcact tccatgaggt aggcgcggcc gacgcgctgg ccgacgtggt cggtactgcg
     1621 gtcgcggccg agcatctggg cttgctcgcc gacgacgcgg tggtgatctg ctcaccactg
     1681 gccgccggca gcggtacggc gcacaccgcg cacgggacaa tcccggtccc ggcacccgcc
     1741 gtgctgcaga tcgtcgccga ccgcggcatg tgcctgactg gcggagacct ggccggggaa
     1801 cgcaccacgc ccaccggggc ggccctgacc gcgacgctgg ccacgcctgg cctgctaccg
     1861 ccgatgacgg tcgacgtggt cggctgcggc gcggggcacc gggacaccgc ggaacgaccc
     1921 aacatcaccc ggttggtgat cggcagcccg gtcgggcagc tcaccggccc gggccccgcc
     1981 gaggccgtca tcgtggtgga gaccaccgtt gatgatctca acccggaatt gtggccaggg
     2041 gtgttgaccg ctgtccgggc ggccggtgcg tgggactgct ggaccacggc aacagtcggt
     2101 cggcacggca ggcctgggca ggtgctcacc gcgctgtgca ccgaagctgc gcgtcccgca
     2161 gtggcggacg cgctgttccg gcacagcacc acgctgggcg tacggtgggc gtcctggcag
     2221 cgcagcacgc ttccccggat caccgtcgaa gtaccggtgg gtccacccgg cgcggagcga
     2281 gagatcatca tcaaggtcgc cgccgggcca ggagggcagc ggaccgccaa acctgagctc
     2341 gccgacgccg agcggatcgc gcaagaactg ggctggccgg tgcgtcgggt atatgaagcc
     2401 gctctgtccg cctaccaccg cgcccatccg gcggatgccg tggcgccccg agataatcag
     2461 tcctgacgct gctcggggca gccgggaagg atcacatgat cacgcgggat ctgcgcgaga
     2521 cctgcttgca gggtggagcc atcgcgaccg tcagggtcga accatgagcg ccgacgacga
     2581 gctcgccggc tcacacgtgc tgctcaccgg agccaccggg tttctggggc aggcgacgtt
     2641 ggagcggctg ctgtccagtt acccggatac tcgagtctcg gtgctgatcc ggccgcgtgc
     2701 cgggcgctgc gcggctgacc ggctcaccgc gctgctgcgc aagaacgtgt tccgctcgtt
     2761 gggcgagcgc ctcggcgagg acgggctggc cggtgtggtg cgcgaccggg tcaccgtcct
     2821 cgagggcgac ctggactcga tcaccctgcc cactgatctc gatgtcgtga tccacggtgc
     2881 gtccgcggtg tccttcgatc caccggtcga tgaggcgttc cgcaccaacg tcgccggtgt
     2941 ggcggccctc tatgaggaac tggctgcggt aggtagttcc ccgcacgtgg tgcacgtgtc
     3001 caccgcctac gtcgcaggta cccgtaaggg gctggtcgtc gaggcggcgc tggaccatga
     3061 cgtggactgg cgccttgagc tggagatggc gttggcggcc cgggctgagg cagagcggga
     3121 ctcccggcgt ccggaggtgc tccagcgggc gctggcacag gcggccgaac agcacgccaa
     3181 ggccggtccg cagtccaccg ctaccgacgc cgaacggctt cgcactgaat gggtcacccg
     3241 gcgtctggtg gactacggcc gtacccgggc gcggagtctg ggttggccgg acgtgtacac
     3301 cctgaccaag gcgttgggcg agcgagttgc cgagcagctg tgggcgcaga caggtaggcc
     3361 gctgtcagtg gtgcgtcccg ccatcgtgga gagtgcgctg cgccacccgt acccggggtg
     3421 gatcgacggc ttcaagatgg ccgacccgct gatcatcgcc ttcggacgcg gagtgctccg
     3481 agagtttcct gggttacccg actccgtgct cgacgtcatc ccagtcgacc tggtggtcaa
     3541 cgccatcctt gcggcagccg cgcggacacc cgtcaccgcg ccagcgtacc tgcacgtcag
     3601 ctcgggggcg agcaacccgc tgacctttca gaacatgtac gcgctgtgcc gggagtactt
     3661 cgacaacaac ccgatgcccg ttgctgaccg cggccacgtc gatccaccga gctggcggtt
     3721 tccgggcagc cagcaggtgg aattgatgct gcgcgccggg gagcaagccg ccgcgctcgc
     3781 ggaacgggtg ctgctggctc tgcccccgtc gaagcgatcc caagactggc tgaccagcct
     3841 gggacgccac cagcaggacc tcgtgcaact gcgcgaatac gccgaccttt acggcgtcta
     3901 cgcccgcgcg gaggtgatct acgacgaccg ccggctacgg gagttacacg ccagcctgcc
     3961 gacccagcga gcggccgaac acggtttcga ccccaccacc atcgactggc gggcctacct
     4021 gcaagacgtg cactgtcccg ccatcactgc cacggtccgc cgggccg
//
LOCUS       DATADN010000431         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_508076, whole genome shotgun sequence.
ACCESSION   DATADN010000431 DATADN010000000
VERSION     DATADN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508076"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1517)
                     /gene="ltrA"
                     /locus_tag="VHH53_05630"
     CDS             complement(177..1517)
                     /gene="ltrA"
                     /locus_tag="VHH53_05630"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX2289669.1"
                     /translation="MSTAAERSGRPARPEDAVIPGLEVSLWERMLSPENLGRALRRVR
                     ANGGAPGVDGMTTDELVPWLREHWVQVRQALDAGTYRPSPVRRVVIPKPGGGERLLGV
                     PTCLDRLIQQAIAQVLTPIFDPDFSGSSFGFRPGRSAHQAVRAARQSIQDGLGWVVDI
                     DLDRFFDRVQFDVLMARVARKVDDRKILKLIRSYLEAGVMVDGIKQATVEGTPQGSPL
                     SPILSNIMLDDLDRELGERGHRFVRYADDLRVFVRSERAAQRVFESVCGVIEQRLRLK
                     VNRDKSSIRHAAQATLLGFGFCLTGSKVGIRVAPKAITRLKDRLRILTGRSWRVSMDY
                     RIGRLNRFITGWMAYFRLADAQQRTFRDLDGWLRRRLRQVRWKEWKTTAAKRHNLRIR
                     GISESNARKWAGSSKGYWRVAGSKVLQVSLPNSYWNRLGLKTMSQTWQRLKPTA"
BASE COUNT          253 a          586 c          484 g          256 t
ORIGIN      
        1 cggtctgctc aggatcagcc gagcgctttc caggtagctg ccgcggtgaa ttttctatct
       61 cccatgccgt caccctaggc ttgccgagga cgcctctttc agtaatcggg tagggggcgg
      121 ggtcgccccg ccccctccca caccaccgga catgcgggcc cgcatccggc ggttcgtcaa
      181 gccgtcggtt tgagacgctg ccaggtctgg ctcatggttt tcaggccgag gcggttccag
      241 taggagttgg gcagggacac ctggaggacc ttggagcccg caacccgcca gtaccccttg
      301 ctgctacccg cccatttccg ggcgttgctc tcggagatac cgcgtatccg caggttgtgc
      361 cgcttggcgg cggtggtttt ccattccttc cagcggacct gccgcaagcg gcggcgcagc
      421 catccgtcca ggtcgcggaa cgtccgttgt tgggcgtccg cgagccggaa gtaggccatc
      481 cagccggtga tgaaccggtt gagcctgccg atgcgatagt ccatcgacac ccgccagctt
      541 cgcccggtca agatccgcag ccgatccttc agccgggtga tcgccttggg ggcgacccgg
      601 atcccaacct tcgagccggt gagacagaac ccgaacccca gcagcgtcgc ctgcgcggcg
      661 tgccggatcg aggacttgtc ccggttcacc ttcaacctca gccgctgttc gatcacgccg
      721 cagacgctct cgaacaccct ctgggcggct cgttcgctgc gcacgaagac ccgcaggtca
      781 tcggcgtacc tcacgaacct gtgaccgcgt tcccccagct cccggtccag atcatccagc
      841 atgatgttgg acaagatcgg cgacagcggg gagccctgcg gggtcccctc caccgtcgcc
      901 tgcttgatcc catccaccat caccccggcc tccagatagc tccggatcag cttgaggatc
      961 ttgcggtcgt ccaccttgcg cgccactcgc gccatcagca catcgaactg aacccggtcg
     1021 aaaaaccggt ccaggtcgat gtccacgacc caccccaggc cgtcctggat gctctgccgc
     1081 gcggcccgca ccgcctggtg ggcagaccgg ccgggacgga acccgaacga cgacccggag
     1141 aaatccgggt cgaagatggg cgtgagcacc tgcgcgatgg cctgctggat cagcctgtcc
     1201 aggcaggtgg gcacccccaa caaacgctcg ccgcccccgg gcttggggat caccacccgg
     1261 cggaccggcg agggccggta tgtgcccgcg tccagggcct ggcggacctg cacccagtgc
     1321 tcgcgcagcc acgggaccag ctcgtccgtg gtcatgccgt ccacaccggg cgcgccaccg
     1381 ttggccctga cacgcctcaa cgcccggccc agattctccg gcgagagcat ccgctcccac
     1441 aacgagacct ccaggccggg gatgacagca tcctcggggc gcgccggccg gccgctacgc
     1501 tccgccgcgg tgctcaacgg acctcaccgt ctgcacccca acgggcccgc gtccgcggga
     1561 tggctgcgat caccgcgac
//
LOCUS       DATADN010000432         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_36387, whole genome shotgun sequence.
ACCESSION   DATADN010000432 DATADN010000000
VERSION     DATADN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36387"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VHH53_05635"
     CDS             complement(<1..1202)
                     /locus_tag="VHH53_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2289670.1"
                     /translation="MDVLAGHVDSADPAIQAHAAMVADLRQRLATARLGGPPKARERH
                     VERGKLLPRDRVDTLLDPSSPFLELSPLAATGMYGDEAPGAGIITGVGRVAGRECVVV
                     ANDATVKGGAYYPMTVKKHLRAQEVALHNHLPCLYLVDSGGAYLPEQDQVFPDREHFG
                     RIFYNQATMSARGIPQIAAVLGSCTAGGAYVPAMSDQAVIVRNQGTIFLGGPPLVRAA
                     TGEVVTAEELGGADLHSRISGVTDHLADDDAHALRIVRSIVETFGPRPPRPWEMIATE
                     EPSMDGIHGVVPVDIRTAYDVREVIARIVDGSRLSEFKAEYGATLVTGFARLHGHPVG
                     IVANNGILFSESALKGAHFIELCDQRGIPLVFLQNISGFMVGREYEAGGIAKHGAKMV
                     TAVATTRVP"
     gene            1284..>1729
                     /locus_tag="VHH53_05640"
     CDS             1284..>1729
                     /locus_tag="VHH53_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2289671.1"
                     /translation="MALPSAASGTPTRRAQILHVAATLFARHGFHGVSIAELGAAVGV
                     SGPALYRHFPGKEALLAEMLVGISEYLLDGGRARARTDDPSQVLTDLVEFHVDFALQH
                     PELITVQDRDLANLPVQVRHQVRALQRAYLQIWVDTLRELIPELSA"
BASE COUNT          274 a          589 c          571 g          295 t
ORIGIN      
        1 gggacccggg tggtcgccac cgcggtgacc atcttagcgc cgtgcttggc gatcccgccg
       61 gcctcgtatt cccggccgac catgaatccg gagatgttct gcaagaacac cagcggaatg
      121 ccgcgctggt cacacaattc aataaaatgc gcgcccttga gcgccgactc gctgaacaga
      181 atcccgttgt tggccacgat gcccaccggg tggccatgca gccgggcgaa gccggtgacc
      241 agggtcgcac cgtactcggc cttgaactcc gacaagcggc tgccgtcgac gatccgagcg
      301 atcacctccc ggacgtcata ggcagtgcgg atatccaccg gcaccacgcc gtgaatccca
      361 tccatcgaag gttcctcggt ggcgatcatc tcccacggcc ggggtggacg gggtccgaaa
      421 gtctccacaa tggaacggac gatccgcagc gcgtgcgcgt cgtcgtcggc gaggtggtcg
      481 gtgaccccgg agatgcggga gtgcaggtct gcgccaccga gttcctcggc ggtgaccacc
      541 tcaccggtgg ctgccctcac cagcggcggg ccgcccagaa agatcgtgcc ctggttgcgg
      601 acgatcaccg cctgatcgct catcgccggc acgtaggcgc cccccgcggt gcaggaaccg
      661 agcaccgccg cgatctgcgg gatgccgcgc gcggacatcg tcgcctggtt gtagaaaatc
      721 cggccgaagt gttcccggtc agggaacacc tggtcctgct cgggcaagta ggccccaccg
      781 gagtcgacga ggtagaggca gggcaggtgg ttgtgcagcg ccacttcctg ggcacgcaga
      841 tgcttcttga cggtcatcgg gtagtaggcg ccgcccttga cggtggcgtc attggcgacg
      901 accacacatt cccgaccggc cacccgcccg accccggtga tgatccccgc gcccggggcc
      961 tcgtcaccgt acatgccggt agctgccagc ggggacagtt ccaagaacgg cgaggacggg
     1021 tccagcagcg tgtcgacgcg gtcgcgcggc aacagcttgc cgcgctcgac atggcgctct
     1081 cgagctttcg gcggccctcc cagccgggct gtcgcgagcc gctggcgcag gtcggcgacc
     1141 attgcggcgt gcgcctgaat ggctgggtct gcggagtcga cgtggccggc cagtacgtcc
     1201 atcgtcgctc ccttctcccc gcgtcagtta gcgcttatta actacgttag cgctcattaa
     1261 catcggccgc tacggtcttg cgcatggcac tcccttcggc agcgagcggc acgccgaccc
     1321 ggcgggcgca gatcctgcat gtcgccgcca cgctgttcgc tcggcacggg ttccacgggg
     1381 tgagcatcgc cgagctcggt gctgcggtgg gcgtcagcgg gcctgcgctg taccggcact
     1441 ttccggggaa ggaggcgctg ctggccgaga tgctcgtcgg catcagcgag tacctgctcg
     1501 atggtgggcg cgctcgcgct cgcactgacg accctagtca ggtcctgacc gacttggtcg
     1561 agttccacgt ggacttcgcc ttgcagcatc ccgagctgat caccgtgcag gatcgtgacc
     1621 tggcgaacct tcccgtgcag gtgcggcatc aggtccgcgc cctgcagcgg gcttacttgc
     1681 agatctgggt ggacacgctg cgcgaactca tccccgagct gtccgccga
//
LOCUS       DATADN010000433         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_655481, whole genome shotgun sequence.
ACCESSION   DATADN010000433 DATADN010000000
VERSION     DATADN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655481"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VHH53_05645"
     CDS             <1..361
                     /locus_tag="VHH53_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX2289672.1"
                     /translation="TEPRYLHQVRELRPDGVVLAADDLPARELPGQGPWRVHYHVPLH
                     WKPGPAEPVQPTSDVLRAVLGAADCEHIEVETYTWDVLPPAQRPRSDADLVAGIAAEL
                     DTARQLLAPVRTGERRE"
     gene            397..1775
                     /locus_tag="VHH53_05650"
                     /pseudo
     CDS             397..1775
                     /locus_tag="VHH53_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982097.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
BASE COUNT          312 a          715 c          652 g          308 t
ORIGIN      
        1 cactgagccc cgctacctgc accaggtccg ggagctgcgg cctgatggcg tggtgctggc
       61 agccgacgac ctgccggccc gtgagctacc cgggcagggc ccgtggcggg tgcactacca
      121 cgtgccgctg cactggaagc ccgggccagc cgaaccggtg cagccgacca gcgacgtgct
      181 gcgggcggtg ctcggtgctg ccgactgcga gcacatcgag gtggagacct acacctggga
      241 cgtactgccg ccggcccagc gtcctcgcag cgacgctgat ctcgtcgctg gcatcgcggc
      301 agagctggac acagcacggc agctgctggc cccggtgcga accggggagc ggcgcgaatg
      361 atccctaccg gcggtcgcga gagcgcggcg atctcgatcc gtccggtgct gctcatcaac
      421 gtcgtcgggc tcactccacc gctgcttgag cacatgccgc ggctgcgcgg gctagcgggc
      481 acgcgggccg ggctgggcac cgtgctgcct gccgtcacct gcagtatgca gtccacattc
      541 cttaccgggc tgctgccctg tgagcacggc atcgtcggca acggctggta cttccgcgaa
      601 ctcggcgagg tgctcctctg gcgccagcac aaccgcctgg tggccggtga gaagctgtgg
      661 gagacggcgc gcgcggcgcg tccgggttac cgtgcggcaa acctgtgctg gtggtacgcc
      721 atgggcgcct ccaccgacat caccgtcact ccccgtccga tctaccattc cgacgggcgc
      781 aagtccccgg actgctatgt ccgcccacca gcgctgcatg acgagctcac cgcggagctg
      841 ggtgccttcc cgctgttcca ctactgggga ccgaccgcgt cgatcgcatc cagccgctgg
      901 atcgtcgccg ccgctcgccg ggtactgcgc accaaccgac ctgagctgac catggtttac
      961 gtcccgcatc tggactacga cctgcagcgt ttcgggccgt ccgggccgca ggctgcggcg
     1021 gcggccgccg aactggacgc ctgcttggcc ccgctgctgg acgacgcact caactccggc
     1081 gtcgccatcg tcgtggtctc ggaatatggc atcaccgacg ctcggcgcgc gatcgacgtc
     1141 aaccgggcgc tgcgagccga ccggctgctg gaggtctaca cccagaaggg catggagtac
     1201 ctggacccgg gacatcccgg ccttcgccgt cgccgaccac cagatcaccc acatctacat
     1261 ccgcgacagt tccgatgtgc cccgggtccg cagcctggtg gccggcttac ccggggttga
     1321 cgaggtgctc gaccgcgaag cgcaggcctg tcacggcctg gaccacccgc gagccggcga
     1381 actcgtcgcg gtcgccgagc cagatgcgtg gttcacctac tactactggc tcaacgacgc
     1441 ccgggcaccg gacttcgctc acgggggtgg aaattcattg caagccgggt tacgaccccg
     1501 ccgagctgtt cttcgaccca cgcgatccgc tggtcaaggc acgtgccgga ctcaacctcg
     1561 tacgcaagaa ggtcggactg cgctacgcca tgcgaaccgt gccactggac ccggcctgcg
     1621 tacgaggcac ccacggcagg cgcccagaca ccccggctgc cggacccatg ctgctgtgta
     1681 gcgacgacct gccaggagtc gagcagcgga tcgcagcgac tgacgtccgc gctctcgtac
     1741 tcgccctcca gggcctccca gcgccgaccc actgaaccaa aaccggccag cagaatgaca
     1801 agccgaaccc tgagattgcg gtggacgggc cccgtgtcgc cgtcatcagc tcgcggcacg
     1861 cgacattagc gaggttgcgg ccgctgtgct gcgggagcgg gatttcgacg gcattcgcgt
     1921 ccgggagctg ttgggggagc gcgatctcac cttcgctgag gcaacccaga tcgtcggcga
     1981 ccgcgtt
//
LOCUS       DATADN010000434         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_655541, whole genome shotgun sequence.
ACCESSION   DATADN010000434 DATADN010000000
VERSION     DATADN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655541"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VHH53_05655"
     CDS             complement(<1..328)
                     /locus_tag="VHH53_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289673.1"
                     /translation="MTPHRREAYLPPTGDAVAQPMNRGRTMMISQPAMLCLTATWQRL
                     TGRATSRAVPATEQPRPESGPAEVIQREVDVASESLLVAIAAGEGENLAAEGHDQTPA
                     PPTRVDV"
     gene            463..1254
                     /locus_tag="VHH53_05660"
     CDS             463..1254
                     /locus_tag="VHH53_05660"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX2289674.1"
                     /translation="MTTSPAGIPTGDRNPAVAALIEIARPARVITAFTGAGISTESGI
                     PDYRGPGGVWERNAPPTLSDFRENLETRRQYWEERRERYPALRDTEPNAGHLALARLH
                     SAGRLSHVITQNIDGLHQKAGSDPDRTIELHGTAHRVRCLDCGASWPAAEIQTRLELE
                     LLPACEICGGMLRAATILFGQQMPEDALRNAFSVARTTDLMLVVGSSLVVQPAARLPQ
                     IAVASGARLAIVNNEPTPLDSLADVVVRSSAGAVLRTLADALLGG"
     gene            complement(1261..>1931)
                     /locus_tag="VHH53_05665"
     CDS             complement(1261..>1931)
                     /locus_tag="VHH53_05665"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX2289675.1"
                     /translation="LSMLTHFKLAGQLAVLRPDSTRVVAGHPVPDPEGPFPHKATHLT
                     LRFGETLAYYSDIRQFGWWRLMPTDAVQAALGTFRFGPEGTGTDEFGRDDLAARLQRR
                     RIAVKQALLDQRVVAGLGNIYVDEALHRARIHPARPANSLDPEEVTRLHEAIGWALDS
                     GIAQGGAAIRRGRAFPRDGFPTVHARRDEPCTICGTAIVKFTLGGRGTYFCPVCQPEV
                     ASAP"
BASE COUNT          329 a          647 c          634 g          321 t
ORIGIN      
        1 agacatcgac gcgcgtgggc ggagcaggcg tctggtcgtg cccctcggca gcgaggtttt
       61 caccctcgcc cgcagcgata gccaccagaa gcgactccga cgctacatcg acttcgcgct
      121 ggatcacttc cgccggcccc gactcgggtc gcggctgctc agtggctggc accgcgcggg
      181 aagtcgcccg gccagtcagc cgctgccacg tcgcggtgag gcacaacatt gctggttggg
      241 agatcatcat ggtgcgacct cgattcatcg gctgtgccac cgcgtcgccg gtcggcggca
      301 ggtaggcttc gcgccggtgc ggggtcaccg ccacgttctg tcgattgagc ggggattctg
      361 gctcccacca gagatggcag cgtaccatcg cgaccgtact gacacaagac cagctgtgac
      421 ctagaggcat ggaccacccg cgctggagaa gggatgcatt cgttgacgac aagccccgcc
      481 gggattccaa ccggggatcg taatccagcg gtcgcggcgc tcatcgagat tgcgcgacca
      541 gcccgcgtca tcaccgcttt caccggagcg ggaatcagca ccgaatccgg cattcccgat
      601 taccgaggac ccggtggcgt ctgggagcgc aacgcgccgc cgaccctcag cgactttcgc
      661 gagaacttgg aaactcgtcg tcagtactgg gaggaacggc gtgagcgcta cccggcgctg
      721 cgcgatacgg aaccgaacgc cggccatctg gcactggcgc ggttgcattc cgcgggccgc
      781 ctctcgcacg tcatcaccca gaacatcgat gggttgcatc agaaggccgg gagtgatccc
      841 gatcgcacca tcgagctgca cggcacggcg caccgggtcc gctgtctcga ctgtggcgca
      901 tcatggccgg cggccgagat tcagaccagg ctcgagctgg agctgctgcc agcttgtgaa
      961 atctgcggcg ggatgctccg cgccgccacg attctgttcg gtcaacagat gcccgaggac
     1021 gcgctgcgaa acgcattctc cgtcgctcgc accactgacc tgatgctggt cgttggcagc
     1081 tcgctggtcg tgcaaccggc agcgcgtctg ccgcagatcg cggtcgccag cggcgcccgg
     1141 ctggccatcg tcaacaacga gccaactccc ctcgacagtc tggcggatgt cgttgtgcgc
     1201 tcgagcgcgg gcgccgtcct ccgtacgctc gcagacgccc tgcttggtgg gtgaccggag
     1261 ttacggcgcg gatgcgacct ccggttggca gaccggacag aagtacgtgc cccggccgcc
     1321 aagcgtgaac ttgacgatgg ccgtgccgca gatcgtacat ggctcatcgc gccgggcatg
     1381 aaccgtcggg aacccatcac gcgggaaggc cctgcctcgc cggatggctg ccccgccctg
     1441 ggcgataccg ctgtccagcg cccagccgat cgcctcgtgg aggcgggtga cctcctccgg
     1501 gtcgagactg ttggccggtc gggccggatg gatccgggcg cggtgcaacg cctcatcgac
     1561 atagatgttg cccagacccg ctaccactcg ctgatcaagc aacgcttgct tgaccgcaat
     1621 ccggcggcgc tgcaagcgag cggcgaggtc atcgcgaccg aactcgtcgg tgccggttcc
     1681 ctcgggtcca aagcggaaag tccctagcgc ggcttggacg gcatccgtcg gcatcagccg
     1741 ccaccagccg aactgccgga tgtcgctgta ataagcgagg gtctcgccga agcggagcgt
     1801 gagatgtgtt gccttgtgcg gaaacgggcc ttcaggatcg ggcacgggat gacccgcgac
     1861 gactctcgtg ctgtctggcc gcaacaccgc taactggcca gccagcttga agtgggtcag
     1921 catgctcagt c
//
LOCUS       DATADN010000435         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1245672, whole genome shotgun sequence.
ACCESSION   DATADN010000435 DATADN010000000
VERSION     DATADN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245672"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VHH53_05670"
     CDS             complement(<1..996)
                     /locus_tag="VHH53_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX2289676.1"
                     /translation="MSPVGHYGSEHGFYTEHDWARLVHEPAKRAALPGSEPERRSPFA
                     RDRARVLHSAALRRLAGKTQVVGPGEGDVPRTRLTHSLEVAQIGRGIAVELGCDPDLV
                     ETAGLAHDIGHPPFGHNGEHALNEVATGCGGFEGNAQTLRILTRLEPKLLDPGTGRGH
                     GLNLTRASLDATIKYPWPRRDGTIKFGFYADDAAVFDWLREGAPEGVQCLETQVMDWA
                     DDVAYSVHDVEDGILAGRISLATLADPVERALLAELAAKHFSVLPSAQLEAAAEGLLT
                     LPVVDALRHYDGSLAAQVALKELTSELVGRFASAVVSATRTRYGNGPLGRYRAHLG"
     gene            complement(993..1616)
                     /locus_tag="VHH53_05675"
     CDS             complement(993..1616)
                     /locus_tag="VHH53_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX2289677.1"
                     /translation="MLPVALRRLILGTVLVGALIVGGTLFRVWQVARVDDRHSADVVV
                     VLGAAQYDGTPSAVLGSRLDHAARLWRDGVAPVIVTVGGRRDGDTYTEAVAGRNYLTS
                     YGIPPDAVVPVGEGSDTLESLRAVARRADTEGWRTAVIVSDPWHSLRARTMADDLGLT
                     AWTSPTRSGPIVQTRNIQAQYIVRESAALLHYRFTHSPAESLSIDLG"
     gene            complement(1633..2676)
                     /locus_tag="VHH53_05680"
     CDS             complement(1633..2676)
                     /locus_tag="VHH53_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX2289678.1"
                     /translation="METVSPRDSTEETRQRRQVGEALRRWAQQGRPSGVVRVLQRHGF
                     GTVATGQLLAGTADGDRVGAVFEGTMDTVALPLLTSAVATPGTTKGHVTESDAVDAGL
                     ACSGGAQLLGHPLPAKAAAALGAALVDGVPSALASTVDGSAVLVAVGPGLTEVIGSLG
                     SADADEIVLTRLRELARIGATATEQGDAAGIPVLIDVWVPVTTMLIVGRGVIGTALAA
                     QCELLDWAVRTETELAPAVAAAKDFTAADVLVLLDHGPAFDAVLMELMRSGRGFCGAL
                     GSRHTQTARRNRLQAAGVTEEELARLHGPVGLDLGAATPAETAVSVVGEVIAVRRGRR
                     GGPLSTATGRIGG"
     gene            complement(2707..>4036)
                     /locus_tag="VHH53_05685"
     CDS             complement(2707..>4036)
                     /locus_tag="VHH53_05685"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX2289679.1"
                     /translation="PSGEIYGGTRSAWDYGPLGVELKENVKRQWWRAMVTGREDVVGL
                     DSSVILPRQVWVASGHVQAFHDPLVECQSCHKRFRADQLAEDYAERTGKDVLEGDLSD
                     VPCPNCGTRGEYTEPREFNMMLKTHLGPVETEEGLHYLRPETAQGIFVNFQNVMMSAR
                     KKPPFGIGQIGKSFRNEITPGNFIFRTREFEQMEMEFFVEPGTDEQWHQYWIDTRTDW
                     YVDLGINRDNLRHYEHPKEKLSHYSKRTVDVEYRFGFSSGQEWGELEGIANRTDFDLT
                     THSNHSGVDLSYYDQATDSRYRPYVIEPAAGVGRPMMAFLLEAYAEDEAPNAKGGVDR
                     RVLLRLDRRLAPVKAAVLPLSRNADLTPKARDLATALRKHWNVDFDDAGAIGRRYRRQ
                     DEIGTPFCVTVDFDSLTDHAVTVRERDTMTQNRVAMDQVEAYLAARLGGC"
BASE COUNT          671 a         1426 c         1301 g          638 t
ORIGIN      
        1 ccccaaatgg gcgcggtagc gccccaacgg gccgttcccg taccgcgtcc gggtcgccga
       61 cacgacggcc gaggcgaagc gacccaccag ctcgctggtg agttccttca acgccacctg
      121 agcggccagc gaaccgtcgt agtgccgcag cgcgtcgacc accgggagcg tgagcaaccc
      181 ctccgcggct gcctccaact gcgcgctggg cagcacgctg aagtgcttgg cggctagctc
      241 ggcgagcaat gcccgttcca cggggtcggc caaggtggcc agcgatatgc ggccggccag
      301 gataccgtct tcgacgtcgt gcaccgagta tgcgacgtcg tcggcccagt ccatcacctg
      361 agtctcgagg cactgcacac cttcaggtgc accctcccgc agccagtcga acaccgccgc
      421 atcgtcggcg tagaagccga acttgatagt cccgtcccgg cgcggccacg gatatttgat
      481 agtggcgtcc agcgatgccc gagtcaggtt gagcccgtgt ccccggccgg ttcccgggtc
      541 gagcagcttg ggttccagcc gggtgaggat ccgcaacgtc tgggcgttgc cctcaaaccc
      601 accacagcct gtggcgactt cgttcagagc gtgctcaccg ttgtggccga acggggggtg
      661 gccgatgtcg tgggccagcc ccgcggtctc caccaggtcc ggatcgcagc ccagctcgac
      721 ggcgatgccg cgcccgatct gggcgacctc cagcgagtgg gtcagccggg tcctgggcac
      781 gtccccctct cccgggccga ccacctgggt cttgcccgcg agcctgcgca gtgccgcgga
      841 atgcagcacc cgggcacgat ccctggcaaa gggactgcgt cgctccggtt ccgatccggg
      901 taacgcggct cgcttggccg gctcatgcac cagccgggcc cagtcatgtt cggtgtaaaa
      961 gccgtgctca ctgccatagt ggcccaccgg actcacccca gatcgatgct cagcgactcg
     1021 gccggggagt gcgtgaaccg gtaatgcagc agggcggcgc tctcccggac gatgtactgg
     1081 gcctggatat tgcgggtctg cacgatcggg ccgctgcggg tgggcgaggt ccacgcggtg
     1141 agcccgaggt cgtcggccat ggttcgggcc cgcaacgaat gccagggatc gctgacgatc
     1201 accgcggtgc gccaaccctc ggtgtccgcc cgacgggcaa ctgcacgcag gctttcgagg
     1261 gtgtcgctgc cttccccgac cggcaccact gcatcggggg gaatgccata agacgtcaga
     1321 taattgcggc cggctacggc ctcggtgtag gtgtcaccgt cccgccggcc gccgaccgtg
     1381 acgatcaccg gcgcgacccc gtcgcgccac agccgcgcgg catgatccag ccgtgagccc
     1441 agcacggccg atggtgtccc gtcgtactgg gccgcgccca acaccacgac gacgtcagca
     1501 gaatgccggt catcgacccg ggcaacctgc cacacccgga acagggtgcc acccacgatc
     1561 agggcaccca ccagcaccgt gccgaggatt agccgcctca gcgcgacggg cagcatggac
     1621 cggaaagacc ggtcatccac cgatgcgacc tgtcgcggtg gacagcgggc caccgcgacg
     1681 ccctcgtcga acagcgatga cctccccgac caccgagacc gcagtctctg ccggcgtggc
     1741 cgcgcccagg tccagaccga ccggcccgtg cagccgggcc aattcctcct cggtgacacc
     1801 cgcggcctgc agccggttac gccgcgcagt ctgggtgtgg cgcgatccca gcgcgccgca
     1861 gaacccccgt ccggaccgca tcagctccat cagcacagcg tcgaaggccg gtccgtggtc
     1921 gagcaggacc agcacgtcgg cggcggtgaa gtccttggcg gcggccactg cgggcgccag
     1981 ctcggtctcg gtacgcaccg cccagtccaa cagctcacac tgcgccgcca aggccgtccc
     2041 gatcactccg cggcccacga tcagcatggt ggtcaccggc acccacacgt cgatcagtac
     2101 ggggatgcca gccgcgtcgc cttgctcagt ggccgtcgcg ccaatccggg ccagctcacg
     2161 cagcctggtc agcacaatct cgtccgcgtc ggcactgccc agcgagccga tcacctcggt
     2221 cagcccgggc ccgaccgcca ccagcacggc ggacccgtcg accgtggagg ccagcgcaga
     2281 tggaacccca tcaacgagcg cggcccccag cgcggcagca gccttcgccg gtagcgggtg
     2341 acccagcaac tgcgcgccac ctgagcaggc gagaccggcg tccaccgcgt cggactcggt
     2401 gacgtgccct ttggtggtgc cgggcgtggc cactgcggag gtcagcaatg gcaacgccac
     2461 cgtgtccatg gtgccctcga acaccgcacc aacccggtcg ccatcggcgg tgccggccag
     2521 cagttgaccg gtggcgacgg tgccgaaacc atgccgctgc aacacccgca ccactccgga
     2581 agggcgtccc tgctgggccc accggcgcag ggcctcacct acttgccggc gttgacgcgt
     2641 ttcctcggtg ctgtccctgg ggctcaccgt ttccatgccg accatgcttc cagaccggac
     2701 ggcggttcag cagccgccca gtcgagccgc caagtaggcc tcgacctggt ccatcgcgac
     2761 ccgattctgg gtcatggtgt cccgctcgcg caccgtcacc gcgtggtcgg tgagcgagtc
     2821 gaaatccacc gtcacgcaga acggggtgcc gatctcatct tgcctgcggt accggcggcc
     2881 gatggcgcct gcgtcgtcga agtcgacgtt ccaatgcttc cgcaacgcgg tggcaagatc
     2941 acgagccttg ggtgtgaggt cagcgttgcg cgacagcggc aacaccgcgg ctttcaccgg
     3001 cgcgagccgg cgatccagcc gcagcaggac gcggcgatcc accccgccct tggcgttggg
     3061 tgcctcgtcc tcggcgtagg cctccagcag gaaggccatc atcggacggc cgacgccggc
     3121 ggcgggctcg atcacatagg gccggtagcg ggagtcggtg gcctggtcgt agtaggacag
     3181 gtccaccccg gagtgattgg aatgggtggt gagatcgaaa tcggtgcggt tggcgatgcc
     3241 ctccagctct ccccattcct gcccggagga gaagccgaac cggtactcca catccacggt
     3301 gcgcttggag tagtgcgaga gtttttcctt ggggtgctcg tagtgccgaa ggttgtcccg
     3361 attgatacca agatccacat accagtcagt gcgagtatcg atccagtact gatgccactg
     3421 ctcgtcagta cccggctcga cgaagaactc catctccatc tgctcgaact cgcgggtgcg
     3481 gaagatgaag ttgcccgggg tgatctcgtt tcggaagctc ttgccgatct ggccaatacc
     3541 gaacggcggc ttcttgcgag ccgacatcat cacattctgg aaattgacaa aaataccttg
     3601 tgcggtctcc gggcgcaggt agtgcaggcc ctcctcggtt tccaccggac caaggtgagt
     3661 cttgagcatc atgttgaact cgcgcggttc ggtgtactca ccgcgggtgc cgcagttagg
     3721 gcatgggacg tcggacaggt cgccctccag cacatccttg ccggtgcgtt cggcgtagtc
     3781 ctcggcgagc tggtcggctc ggaaccgctt atgacagctc tggcactcga ccagtgggtc
     3841 gtgaaaagcc tgaacgtgcc cggaggcgac ccacacctgg cggggcagga tcacggacga
     3901 gtcgagaccg acgacgtctt cgcgaccggt gaccatcgca cgccaccact ggcgcttgac
     3961 gttctccttg agttccacac ccagcgggcc gtaatcccac gccgagcggg taccgccgta
     4021 gatctccccg ctcggg
//
LOCUS       DATADN010000436         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_362131, whole genome shotgun sequence.
ACCESSION   DATADN010000436 DATADN010000000
VERSION     DATADN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362131"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VHH53_05690"
     CDS             complement(<1..531)
                     /locus_tag="VHH53_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289680.1"
                     /translation="MAALVALLAGLSLGTGVLIFRHFQADATATSRLYSGVFGGLNDP
                     RPGAEAEALLRLGEQVRALGLPLVVTDRTGRVTAAANLPFEAALDDPRVKLYAERLDR
                     QNPPISDAAIGTVHYGPLPAQRNLIGLAVLQGLTIAVMVAVAVFAYRSAMSAQRDRLW
                     VAMAREAAHQMGTPLTS"
     gene            complement(563..>1517)
                     /gene="serS"
                     /locus_tag="VHH53_05695"
     CDS             complement(563..>1517)
                     /gene="serS"
                     /locus_tag="VHH53_05695"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX2289681.1"
                     /translation="IDQVPDGDASANRILRSWGKPPELGFQPRAHWDLGISLGVLDLP
                     AGVKITGSGFPLFRGWGAKLVRALANFMLDLHTREHGYLEVAPPYLVNRATLTGTGQL
                     PKFAEDVYASPADDLFLIPTAEVPVTNIHRDEILESKDLPAAYVAYSPCFRREAFSGG
                     KDTRGLIRVHQFDKVELVRIVRPEESAAEHERITSHAEAVLQRLELPYRVVELAAGDT
                     GFASARTYDIEVWAAGVGAWLEVSSSSTFTDFQARRANIRYRPAPKAKPEFVHTLNAS
                     GVAFPRTIIALLENNQAADGSVRVPAALAPYLGVDRLTPRA"
BASE COUNT          265 a          489 c          514 g          249 t
ORIGIN      
        1 gctggtcagc ggagtaccca tctgatgagc ggcctcgcgt gccatggcca cccacagacg
       61 gtcgcgctgt gccgacatcg cgctccggta ggcaaacacc gccaccgcca ccatgaccgc
      121 gatggtcaat ccctggagta ccgcgagccc gatcaggttc cgctgtgccg gcagcggtcc
      181 gtagtgcacg gttccgatgg ccgcgtcact gataggagga ttctgtcggt ccaggcgctc
      241 ggcgtacaac ttgaccctgg gatcatcgag cgccgcctcg aaaggcagat tggcggcggc
      301 agtgacccgg ccggtgcggt cggtgacgac cagaggcagc cccagcgctc gcacctgttc
      361 tcccagccgc aggagcgctt ccgcttcggc ccctgggcgg gggtcgttca atccgccaaa
      421 cactccggaa tacaaccggc tggtggcggt cgcatcggcc tgaaagtggc ggaaaatcag
      481 aacgccggtg cccaggctca gcccagccag cagggccacg agcgccgcca ccatcgtggg
      541 cgccagacgc tggagccgct cgctacgcac gcggggtcag gcggtcgacg ccgagatacg
      601 gcgccagtgc cgcaggcact cgcacggacc cgtcggccgc ttggttgttc tccagaagcg
      661 cgatgatggt gcgcgggaag gcgacgcccg aggcgttgag ggtgtgaacg aattccggct
      721 tggccttggg agcgggccgg taccgaatgt tggcgcggcg agcctggaag tcggtgaacg
      781 tgctcgagct ggacacctcc agccaggcgc caacgccggc cgcccacacc tcgatgtcat
      841 aggtgcgtgc gctagcgaag ccggtgtcgc ccgcagccag ctccactacg cggtagggca
      901 gttccagacg ctggagcacc gcctcggcat ggctagtgat tcgctcgtgt tcggcggcgg
      961 actcctccgg ccgcacgatg cgcaccagct caaccttgtc gaactggtgc accctgatca
     1021 ggcccctggt atccttgccg ccggagaagg cttcccgccg gaaacacggc gagtaggcga
     1081 cataggcggc cggcaggtct ttggattcca ggatttcgtc ccggtggatg ttggttaccg
     1141 ggacttcggc ggtgggaatg aggaagaggt cgtctgccgg ggaggcgtac acgtcttccg
     1201 cgaatttggg aagttggccc gtgccggtga gcgttgctcg gttgaccaga tacggaggcg
     1261 ccacctcgag atagccgtgc tctcgcgtat gcagatccag catgaagttg gccagcgcgc
     1321 gaacgagttt tgccccccag ccgcggaaga gtggaaaacc cgagcccgtg atcttgaccc
     1381 ctgccggtaa atccaggaca ccgagcgaga tccccagatc ccagtgggct cggggttgaa
     1441 agccgagctc cggcggcttt ccccagctgc gcagaatccg gttggcggag gcatcaccat
     1501 ccgggacctg atcgatt
//
LOCUS       DATADN010000437         1654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1245785, whole genome shotgun sequence.
ACCESSION   DATADN010000437 DATADN010000000
VERSION     DATADN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245785"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VHH53_05700"
     CDS             complement(<1..303)
                     /locus_tag="VHH53_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HEX2289682.1"
                     /translation="MTTGLAHRAESAAADIVADITELVGCETHSYDLTALAKGADLLA
                     DLLTSRLGPPASLHRHPGGECGDVLVATYPGQGAGHVAIVGHYDTVWPTGTLFGWPV"
     gene            complement(336..>1654)
                     /locus_tag="VHH53_05705"
     CDS             complement(336..>1654)
                     /locus_tag="VHH53_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HEX2289683.1"
                     /translation="DGLALMVEDAVENFANFPPLATILTVMLGIAVAERSELISTLLR
                     RMVTRVPGKYLTFVLSMTAMVGHVAGDAAYVTLIPLGALVFRAAGRSPVLGCIVAYVS
                     ISAGYDASPSLTATDVLLSSISTAAARTIDPDYVVTPVANYYFGLASSVLIALVITVV
                     TEKVLARRADLEADEPDTDLDAEELGQIEVTAAQRRALRVTGLVALTFVAVLVLAVLP
                     AGSPLRGDGGSVVDSPLFSGMAFILGVFFALLGTVYGRLTGTLPGARDIIAAMAEGIR
                     AMAPILVLFFAISQFLAYFKWTHLGEVLAITGAQVLRSIDVQGWMVLVGIAVLVSFMN
                     LVITSGSALWSLAAPVFIPMLMLIGLSPETTQAVYRVADSVTNCITPMSPYFVMALGF
                     IQRYRKSAGIGTLASYTIPLAAVIWVVWVAFFVAWYLLGIPLGPGA"
BASE COUNT          286 a          542 c          604 g          222 t
ORIGIN      
        1 caccggccag ccaaacagcg tgccggtcgg ccacaccgtg tcgtagtgcc cgacgatcgc
       61 gacgtggcct gccccttgcc ccgggtaggt ggcaaccagc acatcaccgc actcgccacc
      121 gggatggcgg tgcaggctgg ccggcggccc gagcctgctg gtgagcaggt cagcgagcag
      181 gtcggcaccc ttggcgaggg cggtcaggtc gtagctgtgg gtctcgcagc cgacgagctc
      241 ggtgatgtcg gcgacgatgt ccgcggcggc gctttccgcg cggtgggcga gtccggttgt
      301 catggcctgc tttctgtgcg agcggtcagg actgctcagg ccccggggcc gagcgggatg
      361 cccagcaggt accaggcgac gaagaacgcg acccagacga cccagatgac ggccgcgagc
      421 gggatcgtgt acgaggccag cgtgccgatc ccggcggact tgcggtagcg ctgtatgaag
      481 ccgagcgcca tcacgaagta cgggctcatc ggggtgatgc agttggtgac cgagtcggcg
      541 acccggtaca cggcctgggt ggtctccggg ctgagcccga tgagcatcag catcgggatg
      601 aacaccggtg cggccagcga ccacagcgcg gatccgctgg tgatgaccag gttcatgaag
      661 ctgaccagca cggcgatgcc gaccagcacc atccagccct gcacgtcgat gctgcgcagc
      721 acctgcgccc cggtgatcgc gaggacctca ccgaggtggg tccacttgaa ataggcgagg
      781 aactgggaga tcgcgaagaa cagcaccagg atcggagcca tcgccctgat gccttcggcc
      841 atcgcggcga tgatgtcgcg ggcgccgggc agcgtgccgg tcagccgccc gtagacggtg
      901 ccgagcagcg cgaagaacac gccgaggatg aacgccattc cgctgaacag cggcgagtcg
      961 accacgctgc cgccgtcacc gcgcagcggc gaccccgcgg gcagcacggc cagcaccagc
     1021 accgcgacga acgtcagcgc gacgagcccg gtcacccgca acgcgcgccg ctgcgcggcg
     1081 gtgacctcga tctggccgag ttcctccgcg tcgaggtcgg tgtccggctc gtcggcctcc
     1141 aggtcggcgc ggcgcgcgag gaccttctcg gtgacaacgg tgatgaccag cgcgatgagc
     1201 accgaggacg cgagcccgaa gtagtagttg gccaccggtg tcacgacgta gtcggggtcg
     1261 atcgtgcggg ccgcggcggt ggagatcgac gagagcagga cgtcggtcgc ggtcagtgag
     1321 ggcgaggcgt cgtagccggc ggagatcgag acgtacgcga cgatgcagcc gaggacgggg
     1381 ctgcgtcccg cggcgcggaa gaccagcgcg cccagcggga tcagggtcac gtaggcggcg
     1441 tcgcccgcga cgtgaccgac catcgccgtc atcgacagca cgaaggtgag gtacttgccc
     1501 ggtacgcggg tgaccatccg ccgcagcagg gtgctgatca gctcgctgcg ttcggccacc
     1561 gcgataccca gcatcacggt gaggatcgtg gccagcggcg ggaagttcgc gaagttctcc
     1621 accgcgtcct cgaccatgag cgcgaggccg tccc
//
LOCUS       DATADN010000438         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_508455, whole genome shotgun sequence.
ACCESSION   DATADN010000438 DATADN010000000
VERSION     DATADN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508455"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1134)
                     /locus_tag="VHH53_05710"
     CDS             complement(202..1134)
                     /locus_tag="VHH53_05710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX2289684.1"
                     /translation="MGTVLVLRALGVGDLLTAVPAVRGLRAAYPDHRLVLAAPAALAE
                     LVALIGGVDELLPTEGLHELAMPSPDVAVNLHGRGPDSIRILQALHPGVSLTHAHLKH
                     PGVHGPEWETDIHEVHRWCRLLAHFGIPADPADLGLARPAVAAAVHGAVVVHPGCAFA
                     ARHWPSQRYAVVARELAASGLPVVVTGNTDQRPLARQVAAAAGLPQRSVLAGQTTLAQ
                     LAALVAGASLVVCGDTGIAHLATAYGTPSVVLFGPVSPQHWGPPPQRPQHVALWAGST
                     GDTFADRPDPGLLRITTDQVLAAARSVARVTATP"
     gene            complement(1140..1829)
                     /locus_tag="VHH53_05715"
     CDS             complement(1140..1829)
                     /locus_tag="VHH53_05715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2289685.1"
                     /translation="MRPLGNVLITGGASGLGAATVEAVIDAGGRPLVLDRALPAGTVD
                     YMQADLADRVATEQAVADLVATAGGRLDAVFTAAGIDSCGSLGSVPADEWERVVQVNL
                     LGTARVVRAALPYLEQSGGTVVTCSSTLGLKALPDATAYCAAKFGVIGFTRALAAETR
                     GRIGVTLLIPGGMHTPFFDGRDAQYKPPPDAKLNRPEDVAATVVYALRQPPGCQLREL
                     VVCSSEESSWP"
     gene            complement(1844..>2383)
                     /gene="rfaE2"
                     /locus_tag="VHH53_05720"
     CDS             complement(1844..>2383)
                     /gene="rfaE2"
                     /locus_tag="VHH53_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX2289686.1"
                     /translation="SRFVADGGVSSIAAGSEVARPAVTYPQVARSVPDVAARVRHRGG
                     RLVATGGCFDLLHPGHLSLLRQARALGDGLVVCLNSDESVRRLKGPSRPIVPAAGRVA
                     LLEALEPVDAVTVFDEDTPAELLDRLRPDIWVKGGDYTVADLPEAPLVSRYGGQTVII
                     PLVEGYSTSRLVAAAQYTP"
BASE COUNT          381 a          883 c          831 g          288 t
ORIGIN      
        1 gcccgcaagc ccgggtcagc actcgagccc cgaccgaggc ggcatggtcg tcggaaaggt
       61 gttcgcgatc tgctcaggga gatccgcgcc ttgcacagcg tgatccgtcc cgcggccagc
      121 gcggtcatcg tggccgggta ccgcgcagtg aggccctgcg cggggccagc atctcgacag
      181 tatcgaacaa tgagttcggt actacggcgt cgctgtgacg cgagcaacgg accgcgcggc
      241 ggccaggacc tgatcagtcg tgatgcgcag caggccgggg tcggggcggt cggcgaacgt
      301 gtctccggtg ctgcctgccc acagcgccac gtgctggggg cgctgcggcg gcgggcccca
      361 gtgctgcggt gacaccgggc cgaagagaac caccgacggg gtgccgtagg cggtcgcgag
      421 gtgagcgatg ccagtgtcac cgcacaccac cagcgacgcg ccggcgacca gggcggcaag
      481 ctgcgccaag gtcgtctggc cggcgagcac cgagcgctgc ggcagcccgg ccgcggccgc
      541 tacctgccgg gcgagcgggc gctggtcggt gttgccggtc accaccactg gtaacccgga
      601 cgcggccagt tcgcgggcca ccacggcata gcgctgcgag ggccagtgcc gggcggcgaa
      661 ggcgcagccg gggtgcacga ccaccgcacc atgcacggca gcggcaacgg ccgggcgggc
      721 caaccccaga tccgccgggt cagcgggaat gccgaaatgg gcgagcagcc ggcaccaccg
      781 gtgcacctcg tgaatgtcag tttcccactc cgggccatgt acaccagggt gcttcaggtg
      841 ggcatgggtg agcgacacgc cggggtgcag tgcctgcaag atgcggatgc tgtccggccc
      901 gcgaccgtgc aggttcactg ccacgtccgg ggacggcatg gccagctcgt gcagcccctc
      961 ggtagggagc agctcgtcga cccccccgat cagcgctacc agctcagcca acgcggcggg
     1021 tgcggccagt accaggcgat gatccggata ggccgcccgc agtccgcgca ccgccggcac
     1081 cgccgtgagc aggtcgccca cacccagcgc ccgcagcacc aggaccgtac ccacccggct
     1141 cacggccagg atgattcctc cgacgagcag accaccagtt cccgcagctg gcagccgggc
     1201 ggctggcgca acgcatacac cacggtggca gccacgtcct cgggccggtt gagcttggcg
     1261 tccggcggcg gcttgtactg ggcgtcgcgg ccgtcaaaga agggggtgtg catgccaccc
     1321 gggatcagca gcgtcacccc gatccggccg cgggtctccg cggccagcgc ccgggtgaag
     1381 ccgatcacgc cgaacttggc cgcgcagtag gccgtcgcat cgggcaacgc cttgagcccg
     1441 agggtggacg agcaggtcac cacagttccc ccggactgct ccaggtaagg cagcgcggca
     1501 cgcaccaccc gagccgtgcc cagcaggttg acctgcacca cccgctccca ctcgtcggcc
     1561 ggcacgctgc ccagcgagcc gcaggaatcg atcccggcgg cggtgaacac ggcatccagc
     1621 cggccgccgg cggtcgcgac caggtccgca acggcctgct cggtggctac ccgatcagcc
     1681 agatccgcct gcatgtagtc cacggttccg gccggtaagg cgcggtcgag aaccagcggg
     1741 cgcccacccg cgtcgatcac cgcctcgacc gtggccgcgc cgagacccga cgcgcccccg
     1801 gtgatcagca cgttgcccag cggtctcatc cgctctcctt ctgtcatggt gtgtactggg
     1861 cggccgcgac cagccggctc gtggaatacc cttcgaccag agggatgatc acggtctgcc
     1921 cgccgtaccg gctgaccagc ggcgcctcag gcaggtcagc cacggtgtag tcgccgccct
     1981 tgacccagat gtcaggccgc agccggtcga gcagctcagc cggagtgtcc tcgtcgaaca
     2041 ccgtcaccgc gtcgaccggc tccagcgcct cgagcagcgc gacccggccc gccgcgggca
     2101 cgatcggccg gctcggcccc ttgagccggc gcaccgactc atcggagttg aggcacacca
     2161 ccaggccgtc acccagcgcg cgggcctggc gcagcaggct caggtgcccg gggtgcagca
     2221 ggtcgaagca ccccccggtg gccaccagcc gcccaccccg gtggcgcacc cgagccgcga
     2281 cgtccggcac cgaccgggcc acctgagggt aagtcacagc cgggcgggcc acctcgctgc
     2341 cggcagcgat cgaggacacc ccaccgtcgg cgacgaaacg tga
//
LOCUS       DATADN010000439         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_964754, whole genome shotgun sequence.
ACCESSION   DATADN010000439 DATADN010000000
VERSION     DATADN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964754"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VHH53_05725"
     CDS             complement(<1..418)
                     /locus_tag="VHH53_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2289687.1"
                     /translation="MASIEPKAEQYDTGRAVLLAAVAALGGFLFGFDTAVINGAVLAI
                     RETFRMGAGLTGFVVASALLGCAVGAWLAGKLADRIGRIRVMVLAAVLFFLSAIGSGL
                     AYSPYDLTFWRIIGGLGVGAASVIAPAYIAEIAPASI"
     gene            704..1387
                     /locus_tag="VHH53_05730"
     CDS             704..1387
                     /locus_tag="VHH53_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2289688.1"
                     /translation="MVLTRSTAWGAAVVVTMVLFAAGVAGCAGVQSQGDEQREAVTGP
                     FTPSTANPDPSRQIPGVVIESYQDPQHVMPTQRVAYDHSPPFGGSHDGFWAACNGVVY
                     PRPVRNENMVHSLEHGAVWIAYNPDRVAGDAVEALASHVRGQPYTMLSPYPGLATAVS
                     LQSWGHQLAVDSVEDPRIDQFLRALRQNPNTHPELGASCDELGSGRFDQDNPPAFDPS
                     PPGPDAVPQ"
     gene            1495..>1968
                     /locus_tag="VHH53_05735"
     CDS             1495..>1968
                     /locus_tag="VHH53_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX2289689.1"
                     /translation="MLASADSQGFVSLRQPATPRGERYAIGRSLRQRVPRSALGNWSV
                     PGNRADPVQQIIATHEGRLDRLIPVRIGRMITSPYAFLRGTAAIMAEDFAHLPSTGIT
                     PVICGDAHLGNFGFYASPERNLVFDLNDFDEAHPGAWEWDLRRLVTSVWVAGRHNG"
BASE COUNT          352 a          665 c          642 g          309 t
ORIGIN      
        1 ggatcgaggc aggggcgatc tccgcgatgt aggccggggc gatgaccgag gcagcaccca
       61 cacccaggcc cccgatgatt cgccagaagg tgaggtcgta gggggagtag gccagtccgg
      121 agccgatcgc gctgaggaag aacagcaccg cggcgagcac catcaccctg atccgaccga
      181 tccgatcggc cagcttgcca gcaagccagg caccgaccgc gcaacccaac agcgcagaag
      241 ccaccacgaa accggttagt ccggcgccca ttctgaaggt ctcgcggatg gccaataccg
      301 caccgttgat tacagcggtg tcgaagccga acaggaaccc cccgagggcg gccaccgcgg
      361 ccagcaggac cgcccgcccg gtgtcgtact gctcggcctt cggctcgatc gacgccatga
      421 ttctcccggg gcatcagttc gggattcacg ctgctgtgat acctaccatg tgccgggtcc
      481 ggccgccgga tgccaggacg ctcgccacgc cacaccggac ccatcagatc ctcgacgagc
      541 cgtagtggca cacttgcgac ctcttgggcg agggtgggca tcggggccga ttggacggga
      601 atatgacacc aacgcagcaa cgacaaggca ccggcttcgg tccgcttcga gttattccat
      661 cgccatgact aggcgcacat tggggcacgg ccggcctggc gcgatggtgc tgacgagatc
      721 gacggcctgg ggtgcggccg tggtcgtgac tatggtgctg ttcgccgctg gcgtcgcggg
      781 atgtgcgggt gtgcagagtc aaggcgacga gcagcgggag gccgtcaccg ggccgttcac
      841 accatcgacg gccaatccgg atccgtcccg gcagatcccc ggtgtcgtga tcgagtccta
      901 tcaggacccg cagcatgtga tgccaactca gcgggtggcc tatgaccaca gcccaccctt
      961 cggcggttcg cacgacggat tttgggccgc atgcaacggc gtggtgtacc cgcgcccggt
     1021 gcgcaacgag aacatggtcc attccctcga gcacggggcg gtgtggatcg cgtacaaccc
     1081 tgaccgggtc gcgggggacg cggtggaggc gttggccagc cacgtacgag gccagcccta
     1141 cacgatgctc tcgccctacc ccgggctggc gaccgcggta tcgctgcagt catggggaca
     1201 ccagctcgca gtggacagcg tcgaggaccc acggatcgac cagttcctcc gtgctctgag
     1261 acaaaacccc aacacccatc ccgagttggg ggccagctgt gatgagttgg gctccggccg
     1321 gttcgaccag gacaacccgc cagcgtttga ccccagcccg ccggggccgg acgcggttcc
     1381 gcaataaacc gggccgcaca tgggcgccgt catgactcag ctcatgcacc ggctcgcgtg
     1441 atgacgtccg ggcagggcca ccacgacggt ggacgagttc gggaccagca tgtcgtgctc
     1501 gcctctgcgg acagccaggg cttcgtttcg ctgcgccaac cggcgactcc ccgaggggag
     1561 cggtacgcca ttggccggtc gctgcgccag cgggtgcccc gatcagcgct tggcaactgg
     1621 agcgtacccg ggaatcgtgc cgacccggta caacagatca tcgcgaccca tgaaggccgg
     1681 ctggaccggc tgatcccggt ccggattggt cggatgatca cttctcccta cgccttcctc
     1741 cgtggtactg ccgccatcat ggccgaggac ttcgcgcacc taccctccac cgggatcacc
     1801 ccggtgatct gcggcgacgc gcatctgggc aacttcggct tctacgcctc gcccgagcgc
     1861 aatctcgtct ttgacctcaa cgacttcgac gaggcgcacc caggtgcctg ggaatgggac
     1921 ctgcgcagac tggtaaccag tgtgtgggtc gccggccggc acaatggt
//
LOCUS       DATADN010000440         4573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1415402, whole genome shotgun sequence.
ACCESSION   DATADN010000440 DATADN010000000
VERSION     DATADN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415402"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3489)
                     /locus_tag="VHH53_05740"
     CDS             complement(<1..3489)
                     /locus_tag="VHH53_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HEX2289690.1"
                     /translation="MGVGNFVDLTFGRVVPADRVLVCEQSEHTIRWRPGERLHHLFEE
                     LCDRTRHSGRLAVDADDSALTHDQLDARANQLARHLLAQGARSGDRIGLLFDHAVHSY
                     IGMLAVLKINAAYVPLDVGFPADRVSFIVGDADVGMVLSLSHLRDRVGHLPATLLCVD
                     EAAAQISARGDHRLTAAEQGPAVDELAYVIYTSGSTGRPKGVAVEHASICNFVRVAVE
                     VYGIQPRDRMYQGMTIAFDFSVEEIWVPLIAGATLVPKPSGSSLLGDELHAFIQAKQI
                     TALCCVPTLLATLDGDLPSLRFLLVSGEACPQDLITRWHRPDRRFLNVYGPTEATVTA
                     TWTPVHPDRPVTIGVPLPTYSAVILDPAGEQRVLQPGEVGEIGIAGIGLARGYLHRDD
                     LTNRAFIHDFIGIPNNPSGRIYRTGDLGRINNKGQIEYHGRIDTQVKIRGYRIELTEI
                     ESVLLALPGIAQAVVDTYEPEPGTVELVAYYSRRADTTEITAHQIYEHLRQRLPRYMV
                     PAYLQELAVIPMLPSDKADRKNLPPPRGRRGLATQHAYLSPTTDTKKHLADALAQSLR
                     LEHLSVDNHFFDDLGADSLLMAQFCARVRKHTDLPPISMKDVYLHPTIRNLAAFLGDA
                     LPAGTGPQVEAVPAVEPASTARYVLCGALQFLLFLGSVYLGALVLDAGFRWIVSGAGW
                     VDTWQRSLVFSAVTFPVLCALPILAKWVLVGRWKPQKIPVWSLAYVRFWLVKSLIRAN
                     PMVLFVGSPLYVVYLRALGAKIGRGVAIFSGTVPVCTDLLTIGDGTVIRKDSSFTCYR
                     ADAGRIETGPVTLGNGVFVGEETVVDIWTSMGDQAQLGHASALNAGQAVPDGQRWHGT
                     PAQRSDVDYRAVDPTRCGTFRRVFYSLAQLITAVGVTGPLMMALIVTLLEELPWLAAL
                     IGPGQDALTTGALYRDALVVSLVLFFGGVLAGLGYVVTIPRLLNLALKPDTVYPLYGL
                     HFWIQRTIARTTNSRFFTFLFGDSSAIVYYLRALGYDLSRVEQTGSNFGMAVKHETPY
                     LSSVGTGTMVSDGLSFINAHFSSTSFRVSRASIGPRTFLGNNIVYPSQGRTGDNCLLA
                     TKVMVPIDGPLRQDVGLLGSPPFEIPRSVQRDGTFDALKSGDELRHRLGAKNRHNAVT
                     IGLFLAVRWIHFFVGTL"
     gene            complement(3594..>4573)
                     /locus_tag="VHH53_05745"
     CDS             complement(3594..>4573)
                     /locus_tag="VHH53_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2289691.1"
                     /translation="KLCKTATGRGDVISFQGGFHGTTHAAMALTGLVANKAPVPNGVP
                     GIHFFPYSSCSSCPVGLTPQTCTTNCVHYLERALKDPNGGIPLPAAVVMELVQGEGGV
                     VPAQPDFVRRVRELTRELDIPLVVDEVQTGCGRTGTWFTFEQYGIEPDVIIASKALSG
                     IGTPVALILYDQRLDVWAPGAHTGTFRGNQLAFAAGVETIRLFRRDDVLGNVRRRGQQ
                     IARTLGELQNNPWVREVRGRGLMWGIELADPLGSRPAGELAEQVQAAALRRGLILELG
                     GRNDCVVRMLPPLNVTAEVVDTACSIFIEALQECCPSTLWSPGPLMSIN"
BASE COUNT          827 a         1435 c         1531 g          780 t
ORIGIN      
        1 cagggtgccc acaaagaagt ggatccaccg cacggccagg aacagcccga tggtgaccgc
       61 gttgtgccga ttcttggcgc ccagccggtg acgcagctcg tccccgctct ttagggcgtc
      121 gaaggtgccg tcgcgctgca ccgaccgcgg gatttcgaag ggcggggatc ctaagagtcc
      181 gacatcctgt cgcagcggcc cgtcgatcgg gaccatgacc ttcgtggcga gcaggcagtt
      241 gtcgcctgtc ctgccctggg agggatagac aatgttgttc ccgagaaaag tgcgcggtcc
      301 aatcgatgct cgtgacacgc ggaaggaagt gctggagaag tgggcattga tgaacgagag
      361 cccgtcggaa accatcgtgc cggtgccgac ggagctcagg tacggcgtct cgtgcttgac
      421 tgccataccg aagttcgacc cggtctgctc cacgcgggac aggtcgtatc ccaatgcacg
      481 caagtagtac acgatggcgg aactgtcacc gaagaggaac gtgaagaacc tgctgttggt
      541 cgtgcgcgcg atcgtgcgct ggatccagaa gtgcaacccg tacagcgggt acaccgtgtc
      601 tggtttgagg gcaaggttta gtaagcgcgg gatagtgacc acatacccga gtccggcgag
      661 cacaccaccg aagaacagca cgagagagac cacgagcgca tcgcggtaca acgccccggt
      721 cgtcaaggcg tcttgacccg ggccgatcag tgccgcgagc caggggagtt cttcgagcag
      781 cgtgacgatg agcgccatca tcagcggccc tgtcacgccc accgcggtga tcagctgcgc
      841 caggctgtaa aagaccctcc tgaaggtgcc gcagcgggtc ggatcgaccg cccggtaatc
      901 cacgtcggac cgctgcgccg gggtaccgtg ccagcgctgg ccgtcgggaa cggcttggcc
      961 ggcgttgaga gccgacgcgt ggccgagctg ggcttggtca cccattgagg tccagatgtc
     1021 gacgacggtc tcttcgccga cgaatacgcc gttgccaagc gtgaccgggc ccgtctcaat
     1081 ccggccagcg tcggctcggt agcaggtaaa tgacgagtcc ttgcggatga ccgtgccgtc
     1141 gccgatggtc agtaagtcgg tgcagacggg gacggttccg gagaagatcg cgacgccgcg
     1201 gccgatcttc gctcccagcg ctctgaggta gaccacatac agcggcgacc caacgaacag
     1261 caccatgggg ttggcccgga tcagggattt gaccagccag aagcggacgt aggccaggct
     1321 ccagacgggg atcttctggg gtttccagcg accgacaaga acccacttcg ccagtatcgg
     1381 tagcgcgcac agcaccggga aggtcacggc gctgaacacc agcgaacgct gccaggtatc
     1441 aacccaacca gcgccactga cgatccacct gaagcctgcg tccagcacca gcgcgccgag
     1501 atacaccgat cccaggaaca gcaggaactg tagtgccccg cacaggacat acctcgcagt
     1561 gctggcgggt tccaccgctg gcaccgcctc cacctgtggc ccggttcccg cgggaagtgc
     1621 gtcccccagg aacgcggcta ggttgcggat cgttgggtgc aggtagacgt ctttcatgga
     1681 tatcggcggg aggtctgtgt gcttgcggac ccgggcgcag aactgtgcca tgagcaggga
     1741 gtcagcgcct aggtcgtcga agaagtgatt gtcgaccgac aggtgttcta ggcgcagaga
     1801 ttgggctagc gcgtcggcca gatgtttctt ggtgtcggta gtcgggctga ggtaggcgtg
     1861 ttgtgtggcc aggccgcgac ggccacgggg tggtgggagg tttttgcggt cggctttgtc
     1921 gctggggagc atcgggatga ccgcgagttc ttggaggtag gccgggacca tgtatcgcgg
     1981 aagccgttgg cgtaggtgtt catagatctg gtgggctgtg atctcggtgg tgtcggcgcg
     2041 gcggctgtag taggcgacca gctctaccgt gccgggttcg ggttcatagg tgtcgactac
     2101 tgcctgggcg atacctggca gtgcaagcag cacggactcg atctcggtca gctcgatccg
     2161 gtagccgcgg atcttgacct gggtgtcgat ccggccgtgg tattcgatct ggcccttgtt
     2221 gttgatccgg cccaggtccc cggtgcggta gatccggccg gagggattgt tggggatgcc
     2281 gatgaagtcg tggatgaacg cccggttggt gaggtcgtca cggtgtaggt agccccgcgc
     2341 gagcccgatc ccggcgatgc cgatctctcc tacctcgccg ggttgcagca ctcgttgctc
     2401 cccggctgga tcgaggatca ccgcggaata ggtgggtagc ggaaccccga tcgtcaccgg
     2461 ccggtctggg tgcaccggtg tccaggtcgc ggtgaccgtg gcctcggtcg ggccgtagac
     2521 gttgagaaac ctgcggtcgg gccggtgcca ccgggtgatc aggtcctgcg ggcacgcctc
     2581 gccggagacc agcaggaagc gcaggctggg aagatccccg tccagtgttg ccagcagggt
     2641 cgggacgcag cacagcgccg tgatctgttt cgcctggatg aatgcgtgca gctcgtcacc
     2701 cagcaggctg gagccgcttg gtttagggac cagggtggcc ccggcgatca gcggcaccca
     2761 gatctcctcg accgagaagt cgaaggcgat cgtcatcccc tgatacatcc ggtcccgcgg
     2821 ttggatcccg tatacctcga ctgctacccg gacgaagttg cagatgctgg cgtgctcgac
     2881 cgccaccccc ttgggccggc ccgtcgagcc cgaggtgtag atgacatagg ccagctcatc
     2941 gaccgcggga ccctgttctg cggcggtgag ccgatggtca ccgcgcgccg aaatctgcgc
     3001 cgcggcctca tccacacaca gcagcgtggc gggaaggtgc ccgacgcggt ctcgtaggtg
     3061 cgacagcgac aacaccatgc cgacgtcggc gtcgcccacg atgaaagaca ctcgatcggc
     3121 cgggaaaccg acgtccagcg gcacgtatgc ggcgttgatc ttaagcacgg ccagcatgcc
     3181 gatgtaggag tgcacggcgt ggtcgaacag cagcccgatc cgatcacccg accgggctcc
     3241 ttgagccagc agatggcgcg cgagctggtt ggcccgtgcg tccaactggt cgtgcgtcag
     3301 cgcgctgtca tcggcgtcta cggcgagtcg gccgctgtgt cgtgtcctgt cgcacagctc
     3361 ctcgaacagg tgatgaagac gttcaccagg tcgccaccgg atcgtgtgct cagactgctc
     3421 gcagaccaag acgcgatcag ccggcacaac cctgccgaac gtgagatcaa caaagttgcc
     3481 tacccccata ttgacgtgcc cctcgcatca gccgtggccc tacgccgtag tcacaatgtg
     3541 atccgccggc agtggggtta ccgacggcgc ccgcgcgata tacacgcggt cgattagttg
     3601 atcgacatca gcggccccgg tgaccacagg gttgacgggc agcactcctg caacgcctcg
     3661 atgaagatcg aacacgcggt gtcgacgacc tcggcggtca cattgagagg tggcagcatc
     3721 cgcacaacgc agtcgttgcg gccgccgagt tccagaatca acccgcgacg caacgccgcc
     3781 gcctgaacct gctcagccaa ctccccagcc ggccgactgc ccagtggatc agcgagctcg
     3841 atgccccaca tcaggccccg accacgcact tcgcgcaccc acgggttgtt ctggagctcc
     3901 cccagcgtcc gagcgatctg ctggccgcgc cgccgcacgt tgcccagcac atcgtcccgg
     3961 cggaaaagcc ggatcgtttc aacccctgcc gcaaaggcaa gctggttgcc tcggaaggtc
     4021 ccggtgtgcg cgcccggcgc ccacacgtcc aggcgctggt cgtacaggat gagcgcgacc
     4081 ggggttccga tcccgctcag cgccttcgac gcgataatga cgtccggctc gatgccgtac
     4141 tgctcgaagg tgaaccaggt gccggtgcgg ccgcagccgg tctgcacctc gtccaccacc
     4201 agcgggatgt cgagttctcg ggtcagctcg cgcactcgcc ggacgaagtc gggctgggcg
     4261 ggcacgacac caccttcacc ttgcaccaac tccatcacca ccgcggccgg caacggtatc
     4321 cccccgttgg gatccttcag cgcccgctcc aggtagtgaa cgcagttggt ggtgcaggtc
     4381 tggggggtga ggcccaccgg gcagctgctg cacgaggagt acgggaagaa gtggatgccg
     4441 ggcacgccgt tgggaacggg tgccttgttg gcgaccagcc cggtcagcgc catcgcggca
     4501 tgggtggtgc cgtggaagcc accctggaag gagatgacgt caccgcggcc ggtcgcggtc
     4561 ttacacagct tca
//
LOCUS       DATADN010000441         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_508885, whole genome shotgun sequence.
ACCESSION   DATADN010000441 DATADN010000000
VERSION     DATADN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508885"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..877)
                     /locus_tag="VHH53_05750"
     CDS             complement(257..877)
                     /locus_tag="VHH53_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289692.1"
                     /translation="MSAATVSTARHAVIIGSLRLIGASTVLIISRRHAGLMQAVAGTV
                     LWVEIEILVEGGRMDANGYVTFLLVGVALIVIDGQLIARSGRGYLQRPQEGGPGRSMV
                     RLVSALFYLVMLGLLALISLINVDTGLPVRDLVVKLGVLLLVLAAVHGLTIAILLRIK
                     DRRLQEQMTEGIAESRYSQGVQGPTVHPVNDGDNGGNRSSSTESRW"
     gene            complement(1011..1616)
                     /locus_tag="VHH53_05755"
     CDS             complement(1011..1616)
                     /locus_tag="VHH53_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237039.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX2289693.1"
                     /translation="MPELSELRKQATSCTACELYAGATQTVFGAGREGAPIMLAGEQP
                     GDQEDRQGQPFVGPAGKLLDQALQRAGISREDAYVTNVVKHFRFTQRGKRRIHQTPGI
                     EHVRACLPWLRAELQAVDPKMLVTLGATAAKSLLGSSFRLTHHRGELLEFDGLPLVAT
                     VHPSAVLRAPDERRDEMFDGLVADLELAGRTAASFQAADPR"
     gene            complement(1721..2293)
                     /locus_tag="VHH53_05760"
     CDS             complement(1721..2293)
                     /locus_tag="VHH53_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2289694.1"
                     /translation="MSLPIQRRRYPARRWDPFASLPGFDDLFDQMNRMLTNAFPDVAR
                     ISVNSWSPPVDIQETDDSFVIEADMPGVRPEDVTVDLQGKELRIGGEYGTAQQQGEGE
                     ASEQQRTNRTGRFDYRITLPGEVTSESCTADLENGVLKLRLPKVSQGTRQRIPVQAGR
                     ARQLGSGQQAETGNTTEATSDASASAGEKQ"
BASE COUNT          425 a          856 c          769 g          440 t
ORIGIN      
        1 gtgactgtct tgcttgtcca cctcggtcgg accgcagtat ggggcagcac acccacaatt
       61 tcggcctagg ctggccgtag ggagggtccc ccagaaccga atccatgacc ggcgctgtga
      121 cggtcagact ccgtgaccgt aagcctccgt gacggtcaca ccgtgacgaa ccggtgtccg
      181 agccgacgcc gcgggtgagc tccaggacac gacacacggt gcacaagagc ccgacacgcg
      241 cgggaatgct acgtagtcac caacgcgact cggtggaact cgagcgattt cccccattgt
      301 cgccgtcatt taccgggtgg acggtggggc cctgaacacc ctgactgtag cggctttccg
      361 cgataccctc cgtcatctgt tcctgcaagc ggcggtcctt gattctcagc aggatcgcta
      421 tcgtcaaacc gtgcacggcg gcgagcacca gcaagagcac tccgagcttg accacaagat
      481 cgcgaacagg aaggccggtg tccacattga tcaacgagat cagcgccagt aaacccagca
      541 tcaccagata aaagagcgcc gagaccagtc ggaccatgga gcgacccggt ccaccctcct
      601 gaggcctctg cagatatccg cgcccactcc gggcgatgag ttgcccgtcg atcacgatga
      661 gcgccacgcc aacgaggaga aacgtcacgt agccgttggc gtccatgcgg cccccttcga
      721 cgagaatctc aatctctacc caaaggacag tacccgctac ggcctgcatc aaaccagcgt
      781 gacggcggga tatgatcaag acggtggacg caccgatcaa ccgtagagag ccgatgatga
      841 ccgcgtgacg cgccgtgctg accgttgccg cgctcactgt cccccggtgc accggcgctc
      901 tacttccgcc agcactccag ccagtgtgac tccgctgcgc gcggccgtcc ccgggttaca
      961 gcagggccta catcaccgtg gctaccccgc agggtgccgc accgttgacg tcaccttggg
     1021 tcagctgcct ggaagctggc cgcggtacgc ccggccaatt ccaggtcagc gactagcccg
     1081 tcgaacatct cgtcgcggcg ctcgtcgggg gcccgcagta ccgctgacgg gtgcaccgtc
     1141 gccaccagcg gcagcccgtc gaactccagc agctcaccgc gatggtgagt gagccggaac
     1201 gatgagccga gcagggactt ggcggccgtc gccccaaggg tcaccagcat tttgggatcg
     1261 accgcctgca gctcggcacg cagccacggc aggcaggccc gcacgtgctc gatgccgggt
     1321 gtctggtgga tccgtcgctt gccccgttga gtgaagcgga aatgcttgac cacattggtg
     1381 acgtaggcgt cttcccggga gatgccggcc cgttgcagcg cctgatccag cagcttgccg
     1441 gctggtccta cgaacggctg cccctggcga tcctcctgat ccccgggctg ctcaccggcc
     1501 agcatgatcg gtgcgccctc gcgcccggcg ccgaaaaccg tttgggtcgc gccggcgtac
     1561 agctcgcacg ctgtgcaact ggtggcctgc ttgcgcagct cgctcagctc cggcatgccg
     1621 acggtgtacc ccgcagcccg gaatgtctaa ccctccgccg acacgccgcc agaacgcagc
     1681 ctgagcggct agccgctcag gctgcacatc gcctactgct ttactgcttt tcgccggcac
     1741 tcgcgctggc gtcgctggtc gcctcggtgg tattgccggt ctcagcttgc tgtccggagc
     1801 cgagctggcg tgctcgaccg gcttgcacgg ggatgcgctg gcgggtgccc tgggaaacct
     1861 tgggcagccg cagcttcagg acgccattct cgaggtcagc ggtgcacgac tcgctggtca
     1921 cctcgcctgg cagcgtgatc cggtagtcaa accggcccgt ccggttggtt cgttgctgct
     1981 cgctggcctc accctcgccc tgctgctgcg cagtgccgta ctccccgccg attcgcagct
     2041 ccttgccctg cagatcgacc gtcacgtcct ccggccgcac tccaggcatg tcggcctcga
     2101 tcacgaacga gtcgtcggtc tcctggatgt ccaccggtgg cgaccaggag ttgacgctga
     2161 tcctggcgac gtccgggaac gcattggtca gcatccggtt catctggtcg aacaagtcgt
     2221 cgaacccggg caagctggca aacggatccc accggcgagc cgggtagcgc cgtcgttgga
     2281 ttggcagtga catcgcggtt ctcctctcgt gagcctccct gtggagggtt cccggacgag
     2341 gtcggcctag tcgctccgag catcaggttc aggaagaact ttgcctggtc agcgcacgcg
     2401 gtctgctgcc ctggagctct cggcacagga caccggccgt atttccttcc ccggtgaacg
     2461 ggtagacgcg gggtgtggcc tacaacgtgg
//
LOCUS       DATADN010000442         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1115322, whole genome shotgun sequence.
ACCESSION   DATADN010000442 DATADN010000000
VERSION     DATADN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115322"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..>1833
                     /locus_tag="VHH53_05765"
     CDS             222..>1833
                     /locus_tag="VHH53_05765"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HEX2289695.1"
                     /translation="MPRSPFVRDVPAAQALDAWRDAWAAAGCPERVSTERLSLGEAIG
                     RVTAEPVWARRSSPAFDSAGMDGIAVRAVDTVGASETAPVTLTDFDVIDTGDPLPEGR
                     DAVVMREHVHLSGEGAELIAATPPYQHVRSIGEDVAAGELLLPQGHRLRAVDIAAAAA
                     AGVTEILVRRRPVVAVLPTGDEIRPLGTEPGIGEIPDTNSLMLAGQAGEHGCDVLSLP
                     IEPDDPDRIAAAVLTAAERADLVLIIAGSSAGRGDHTASVVARVGTLAVHGVAVRPGH
                     PVVLGTAGTTPVLGAPGYPVSAALTFEIFAAPLLAALQGTTTPARSTARARLARKLAS
                     PLGKDDWVRVRLGRVGGQVVATPLPRGAGVLTSLVRADGLLVVPAGLEGYHPGADVEV
                     QLLRGLAEIDRTIVAIGSHDLVLDLAASALRGADPGVTLASSNVGSLGGLVALRDGLC
                     HLAGSHLLDPETGEYTLPYLPRVFGDALAVAEVAVVRLVHRDQGLIVAPGNPLGITGL
                     ADLPEHSYVNRQRGAGTRMLLDQELRRLE"
BASE COUNT          236 a          637 c          678 g          282 t
ORIGIN      
        1 gtacgacgac gagaccgacc tcctcaacct ctttggctga agtagctacc agctccgccg
       61 gtcgtggggc ggtggcctgt accccatcgg tgagtgcagg ccaccgtcgg cgtcagttct
      121 cgccggcgac ggtgggcccc agacttctcc agtagctaac tggatggatg caggcggccg
      181 gtcgaaggtc ctctggctat cccccgggta gcgtcccagc tgtgccgcgc agcccgttcg
      241 tccgtgacgt cccggcagcc caggcgttgg acgcgtggcg cgacgcctgg gcagccgcag
      301 gctgcccaga gcgggtgagc accgagcgat tgagcctcgg cgaggcgatt ggccgggtca
      361 cggccgagcc ggtgtgggcc cggcggtcct ccccggcatt cgactccgcg gggatggatg
      421 gcatcgcagt gcgcgcggtg gacaccgtgg gcgccagcga gacggcaccg gtcacgctga
      481 ccgacttcga cgtcatcgac accggtgacc cgctgcccga gggccgggat gcggtggtga
      541 tgcgcgagca tgttcatctg agtggcgagg gtgctgaact gatcgcggca acgccgccct
      601 accagcacgt ccgctctatc ggggaggacg tcgcggccgg ggagctgttg ctgccgcagg
      661 gccaccggtt acgggcggtg gatatcgcag cggcagccgc agcgggcgtc accgaaatcc
      721 tggtgcgccg ccgcccggtg gtcgcggtgc tgcccaccgg cgacgagatc cggccgttgg
      781 gcaccgagcc gggtatcggc gagattcccg ataccaactc actgatgctc gccggccagg
      841 ccggcgaaca cggttgcgat gtgttgtcgc tacccatcga gccggacgac ccggaccgca
      901 tcgcagccgc ggtgctgacc gctgccgagc gcgccgatct ggtgttgatc atcgccggat
      961 caagcgccgg ccggggggac cacaccgcgt cggtggtcgc ccgggtgggc acgctggcag
     1021 tacacggtgt ggcggtgcgc ccggggcatc ccgtggtgct cgggaccgcg ggcaccacac
     1081 cggtgctcgg tgcgcccgga tacccggtct cggcggcgct gaccttcgag atttttgccg
     1141 cgccgctgtt ggcggccctc caaggcacga ccacaccggc ccgctccacc gcccgagccc
     1201 ggctggcccg caagctggca tccccgctcg ggaaagacga ctgggtgcgg gtacggctgg
     1261 ggcgggtcgg cgggcaggtc gtggccaccc cgctgccgcg cggggccggc gtgctgacct
     1321 cactggtgcg ggccgacggg ttgctggtcg tgccggccgg gctggagggt taccaccctg
     1381 gcgcggatgt cgaggtgcag ttgctgcgcg ggctggctga gatcgaccgc accatcgtgg
     1441 cgatcggttc gcatgatctg gtgctggacc tggccgcctc ggccctacgc ggcgccgacc
     1501 cgggcgtgac gctcgcgtcg tccaacgtcg gctcgctggg cgggctggtg gcgctgcgcg
     1561 acgggctgtg ccacctcgcc ggctcacacc tgctcgatcc cgagaccggc gagtacaccc
     1621 tgccctactt gccgcgggtc ttcggcgatg ccctcgcggt cgccgaggtg gccgtggtcc
     1681 gcctggtgca ccgcgaccag ggcctgatcg ttgcgcctgg caacccgctc ggcatcaccg
     1741 gtctggcgga cctgcccgag cactcgtacg tcaaccgcca gcgcggcgcg gggacccgga
     1801 tgctgctcga ccaggaactg cgccggctgg aga
//
LOCUS       DATADN010000443         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_37154, whole genome shotgun sequence.
ACCESSION   DATADN010000443 DATADN010000000
VERSION     DATADN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37154"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1826
                     /gene="glmU"
                     /locus_tag="VHH53_05770"
     CDS             351..1826
                     /gene="glmU"
                     /locus_tag="VHH53_05770"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX2289696.1"
                     /translation="MLPDGSLTTAIVLAAGEGTRMHSAIPKVLHPMAGRPLVEHAVRA
                     AAGLAPEHLVVVIGHGRDAVHPHLRTLATTLGRDLRAAVQAQQHGTGHAVCCALDVLP
                     AGLSGTILVSYGDVPLLDTGTLYGLVAQHHHTGHAMTVLSAVVTDPTGYGRVLRDHSG
                     AVAGIVEHRDATPAQRAITEINSGVYAFDAVALRDALSQLSSANSQGELYLTDVLALV
                     RAGGLPVGVHTCPDPWLVAGVNDRVQLAALGAEYNRRLLEHWMRAGVTVVDPASVWLD
                     ADVQLAPDVVLHPGVQLHAGTAVGPGAAIGPDSTLSDCTIGAGATVVRTHAVGAVVAD
                     AAQIGPFAYLRPGSQIGTGAKVGTFVETKNAQLGPGAKVPHLSYVGDATIGEGSNVGA
                     ATVFVNYDGVAKHRTTVGAHARTGADNMFVAPVAIGDGAYTAAGSVITQDVPAGALAV
                     ARGRQRNVEGWVSRRRPGTPAAQAAQRAQQADGDPARQERT"
     gene            1838..>2389
                     /gene="prs"
                     /locus_tag="VHH53_05775"
     CDS             1838..>2389
                     /gene="prs"
                     /locus_tag="VHH53_05775"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX2289697.1"
                     /translation="MSAISKKNLMLFSGRCHPELAEEVAKHLDVAVTPQSAYSFANGE
                     IFVRFEESVRGCDAFVVQAHAAPINDAIMEQLIMVDALKRASAKRITAVMPFWGYSRQ
                     DKKHRGREPISARLIADMFKVAGADRIMTVDLHTAQIQGFFDGPVDHLFALPVLADHI
                     RRTYADHELAVVSPDSGRVRLAER"
BASE COUNT          389 a          827 c          814 g          359 t
ORIGIN      
        1 tggacccggc cacgtctgcg gacctgcaaa gttcgtagtc aacgacacgg agcgtaggct
       61 tttggttccg atggtccagg ttcgaatcct ggcggcggag catcgtacac attgtcttga
      121 ccaggcggtg tgccgcgatc acccggacgc tccggccgaa cggctcggct gtcgatttcg
      181 ggaagctgag tgcactccag ggtgatggag acagtgaaac cccagcgtgg cgaccgggtg
      241 aacaacgaat gccacggcgt cggttcgccg aaccagctcc ccccgtagga tccgatctgt
      301 catcagccga atcgagatcg actgcaccaa gatcggaaag gggcaggccg atgctcccag
      361 acgggagcct cacgacggcg atcgtgctgg ctgccggcga gggcaccagg atgcattcgg
      421 cgatccccaa ggtgctgcac ccgatggccg gccgccccct ggtcgaacat gcagtgcggg
      481 ccgccgccgg gctggcgccc gagcacctgg tcgtggtgat cggccacggc cgcgacgccg
      541 tgcaccccca cctccgcacg cttgccacca cgctgggccg cgatctgcgc gctgcggtac
      601 aggcgcagca gcacggcacc ggccacgccg tctgctgtgc gttggacgtc ctgccggccg
      661 gattgtccgg gaccatcctg gtcagctacg gcgacgtgcc gctactggac accggcacgc
      721 tgtacgggct ggttgcccag caccatcaca ccgggcacgc aatgaccgtg ctgtccgcgg
      781 tcgtgaccga ccccaccggc tacgggcggg tgctgcgtga tcactcaggt gcggtcgccg
      841 gcatcgtcga gcaccgcgat gccacccccg cgcagcgggc catcaccgag atcaactccg
      901 gggtgtacgc cttcgatgcc gtcgcactgc gcgatgcgct gagccagctg agttccgcca
      961 acagccaggg cgagctgtac ctgaccgatg tgctggctct ggtccgggct ggcgggttac
     1021 cggtcggcgt ccacacctgc ccagacccgt ggctggtggc cggggtgaac gaccgggtgc
     1081 aactggccgc cctgggtgcg gagtacaacc ggcggctgct ggagcactgg atgcgcgccg
     1141 gggtcaccgt cgtcgacccg gcctcggtat ggctggacgc cgacgtgcaa ctcgcccccg
     1201 acgtcgtgct gcatccgggg gtacagctgc acgctggcac agcggtggga cccggtgccg
     1261 cgatcgggcc ggactcgacc ctgtccgact gcaccatcgg cgcgggcgcc accgtggtgc
     1321 gcacccacgc ggtcggcgct gtcgtagccg acgcagcgca gatcggcccg ttcgcctacc
     1381 tgcggccggg cagccagatc ggcaccggag ccaaggtcgg caccttcgtg gagaccaaga
     1441 atgcgcagct aggaccaggg gcgaaagtgc cgcacctgag ttacgtcggg gatgcgacga
     1501 ttggcgaggg cagcaacgtc ggcgccgcga ccgtgttcgt caactacgac ggggtggcca
     1561 agcatcgcac gaccgtaggt gcgcacgccc ggaccggtgc ggacaacatg ttcgtggccc
     1621 cggtggccat tggcgacggc gcctacaccg cggccggctc ggtgatcacc caagacgtgc
     1681 cggcgggcgc gctggcagtg gcccgcggcc gccagcgcaa tgtcgagggg tgggtgagcc
     1741 ggcgccgccc gggaactccc gcggcgcaag cagcgcagcg cgcacaacaa gccgacggtg
     1801 acccggcacg gcaggagcga acatgagttt ttccccgatg tcggcgatct cgaagaagaa
     1861 cctgatgctc ttctccgggc gttgccaccc tgaactggcc gaggaagtgg ccaaacacct
     1921 cgatgttgcg gtcaccccgc agtcggccta ctccttcgcc aacggtgaga tcttcgtccg
     1981 gttcgaggag tccgtccgcg gctgcgacgc ctttgtcgtg caggcccacg ccgccccgat
     2041 caatgacgcc atcatggaac agttgatcat ggtggatgcg ctcaagcgtg cgtcggccaa
     2101 gcggatcacc gccgtgatgc cgttttgggg ctacagccgc caggacaaga agcaccgggg
     2161 acgcgagccc atctccgccc ggctcatcgc ggacatgttc aaggtcgccg gcgcggaccg
     2221 gatcatgacg gtggacctgc acaccgcgca gatccaggga ttcttcgacg gccccgtcga
     2281 ccacctgttc gcgctgccgg tgctcgccga ccacatcagg cggacgtacg ccgaccacga
     2341 gctggccgtc gtctcgcccg actccgggcg cgtccggctc gccgagcgc
//
LOCUS       DATADN010000444         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_193531, whole genome shotgun sequence.
ACCESSION   DATADN010000444 DATADN010000000
VERSION     DATADN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193531"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VHH53_05780"
     CDS             <1..1576
                     /locus_tag="VHH53_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289698.1"
                     /translation="RQVAADFRIGDDNASAVAEICRRLDGIPLAIELAAARVRVLAPG
                     QIAAGLSNRFGLLTGGVRGAPARQRTLEASLDWSYDLLDDVQRLALARLSVFAGSFEL
                     DAAGAVIAGEGIDRGDALDLVAGLVEQSMVQVTQRHGNARYRLLETIRVYARERLSEM
                     DDPDRVRGRHLEFYAALAGRAHAGLTSGQPEPWVARLTADLDDLRAAMDWAVAAKNLR
                     ALVDLTEPILRFWFERGLSREVHQQLHDAAVAPGVPDDERVRGLQAAAALAFASCEPA
                     SCHRAASKAVAAARAADLGGALAVGLAHRALSGVLAGLSTGEQVDTDVEQALDLAKRC
                     GDASTHAHVLQFAGAALLHSRSIDAGYRLFQQGVQVCEANDLVFHTPAAHAGHVVWSV
                     WSGRLDRSHRHARHAVELSRQVGRPGWEAVGLTGLAAVAVLQGDHVRAQEWLSEAQAV
                     LGPRALEGTYYGMWLRHWLALSAYASGDLDPARAAAEEIVRIGRGGGSRLDEAIGEWL
                     LGMVAQSQQRHDDARG"
BASE COUNT          225 a          518 c          650 g          289 t
ORIGIN      
        1 ccggcaggtg gctgcggact ttcggatcgg tgacgacaac gcttcggcgg tggccgagat
       61 ctgccgccgc ctggatggga tcccgttggc gatcgagctg gcggcggcgc gtgtgcgcgt
      121 gttggcaccg ggccagatcg cggccgggtt gtcgaaccgg ttcgggttgc tgaccggcgg
      181 ggtgcggggc gcgccggcac gtcagcgaac gctcgaggcc tctctggact ggagctatga
      241 cctgctcgac gacgtgcagc ggttggcgtt ggcgaggctg tcggtctttg ccgggtcgtt
      301 cgagctggat gctgccgggg cggtgatagc cggggaggga atcgaccgag gcgacgccct
      361 tgacctggtc gctgggctgg tcgagcaatc gatggtgcag gtcacccagc gccacggcaa
      421 tgcgcgctac cgactcttgg agacgatccg ggtgtatgcc cgggagcggc tgtctgagat
      481 ggacgatccg gatcgggtgc gtggccgcca tctggagttc tatgccgcgt tggcgggacg
      541 ggcgcacgcg ggtctcacga gtgggcagcc tgagccctgg gtggcgcggc ttacggccga
      601 tctcgatgac ctgcgcgccg cgatggattg ggctgtggcg gcgaaaaatc ttcgggcgct
      661 tgttgacctg accgagccga tcctccgctt ctggttcgaa cgcggcctgt cccgggaggt
      721 gcatcagcag ctgcacgacg cggccgtagc accgggcgtc cccgacgacg agcgcgtgcg
      781 ggggttacag gctgcggcag ccctcgcctt tgccagttgt gagcctgcca gctgccatcg
      841 cgcagcgagt aaggcggtcg cagcggcacg cgccgctgat ctgggcggcg cgcttgcagt
      901 cgggttggct caccgcgccc tgtccggggt gctggcgggg ttgtcgaccg gtgagcaggt
      961 ggacactgac gtcgagcagg cgctcgacct cgcgaagcgg tgcggggatg cgtcgaccca
     1021 cgcccacgtc ctgcagttcg cgggggcagc gctgctgcac agccgctcga tcgacgccgg
     1081 ctaccggctg ttccagcagg gcgtgcaggt gtgtgaggcc aacgacctcg tgttccatac
     1141 gccggccgcc cacgcgggcc atgtggtgtg gtcggtgtgg tcagggcggc tcgatcggtc
     1201 acaccgacac gctcgacatg ccgtcgaact cagccggcag gtcgggcgtc ccgggtggga
     1261 ggcggtcggg ctcaccgggc ttgcagcagt tgcggtcctg caaggcgatc acgtccgggc
     1321 gcaggagtgg ctgtccgagg cacaggcggt actgggacca cgagccctcg agggaaccta
     1381 ttacgggatg tggttgcgtc actggctggc cctctcggcc tacgcgtccg gcgatctgga
     1441 ccctgctcgg gccgcggccg aggagatcgt gcggatcggc cgtggtggcg gcagccgttt
     1501 ggacgaggca atcggcgagt ggctgctagg catggttgcg caaagccagc agcgccacga
     1561 cgatgcgcga ggctgagcaa cgaccgaccc agcgtatggg ggagccttgg gtcggtcgac
     1621 actgcgcggc tggtccccag gtgccctcgc gcctcctcgt ggcgcagctc tctttgcgca
     1681 ac
//
LOCUS       DATADN010000445         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_509146, whole genome shotgun sequence.
ACCESSION   DATADN010000445 DATADN010000000
VERSION     DATADN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509146"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VHH53_05785"
     CDS             complement(<1..808)
                     /locus_tag="VHH53_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2289699.1"
                     /translation="MLLYITRRLLWIIPVLFVASIVTFWLMHAAPGSPWNREGRQLDP
                     ELVASLNERFALDQPLPVQYVKWLTGVLQGDFGTSYATGVLGGQQSPFRIETADVAEL
                     IGPAFLPSLTLCGLAFLLATLLGVGLGVLAAWNHNRLVDHLATGVMLLGMIGPPFLIA
                     VLLQILFDPHTVRDTGLLPITGWETPRHWVLPTLALAGLPMAMIGRFTRASMLEVLTE
                     PYVRTAHSKGLPARRVAVIHVLRNALIPVVAVFGPVLAVLITSSIVIERVF"
     gene            complement(861..>1655)
                     /gene="pgl"
                     /locus_tag="VHH53_05790"
     CDS             complement(861..>1655)
                     /gene="pgl"
                     /locus_tag="VHH53_05790"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX2289700.1"
                     /translation="GSRAGGRRVTHRFEVLPDVQSVAAATADRLIGAGRNAIRRRGRF
                     ILALTGGTTPLAVCPLLVVPPRVRMLDWSRVEFFFGDERAVPPNDPESNYNTARLALL
                     DYLPGLRMDQVHRMPCELQDLDAGAWQYERELREVIGGEGIPRFDLIWLGMGADGHTA
                     SLFPGTAALGERERLVVANWVPSLDAWRMTLTYPVLNAARDAMFVVTGEDKAPAVAAV
                     RSGSPDPPAARVRARRTVWLVDEAAAGSPASPGADERQAATAGSIR"
BASE COUNT          282 a          572 c          592 g          209 t
ORIGIN      
        1 cgaagacgcg ctcgatgacg atcgagctgg tgatcaggac ggccagcacc gggccgaaca
       61 ccgccaccac gggaatgagc gcgttgcgca ggacgtggat caccgcaacg cgccgggcag
      121 gcaacccctt gctgtgggcg gtgcgcacgt atggctcggt gagcacctcg agcatcgagg
      181 ctcgcgtgaa gcggccgatc atggccatcg gcagtccggc cagggccagc gtgggcagca
      241 cccagtgccg cggcgtctcc cagccggtga tgggaaggag gccggtgtcc cggaccgtgt
      301 gcgggtcgaa caggatctgc agaaggacgg cgatcaggaa cggtggaccg atcatcccca
      361 gcagcatcac cccggtcgcc aggtggtcca ccaggcgatt gtggttccag gcggcgagaa
      421 cgcccagccc cacgccaagc agggtcgcga gcaggaacgc cagcccgcac agcgtcaggc
      481 tgggcaggaa ggctggcccg atgagctcgg cgacgtcggc cgtctcgatg cggaacgggc
      541 tctgctgacc gcccaggacg ccggtcgcgt acgaagtgcc gaaatcgccc tgcagcacgc
      601 ccgtgagcca cttgacgtac tgcaccggca gcggctggtc caaggcgaag cgctcgttga
      661 gcgaggcgac cagctccgga tccagctgcc ggccctcccg gttccacggg ctgccgggag
      721 cggcatgcat gagccagaac gtgacgatcg aggccacgaa caggacaggg atgatccaga
      781 gcaggcggcg agtgatatag aggagcatgc cttccttggt tcgagctgcg ggggacggcc
      841 cccacagcct tgccccggac ttacctgatg ctacccgccg tggcggcctg gcgttcatcg
      901 gcgcccgggc tggcgggact gccggcagcg gcttcgtcca ccagccacac ggtgcgccgc
      961 gcgcgcacgc gtgccgcggg cgggtcaggc gagcccgagc gcacggcggc cacggccggg
     1021 gctttgtcct cgccggtgac cacgaacatc gcgtcccggg cagcgttcag aaccgggtag
     1081 gtgagcgtca tgcgccaggc atcgagtgat ggcacccagt tggcgaccac caggcgctcc
     1141 cgctcgccca gcgccgccgt gccgggaaac agggacgcgg tatgaccatc ggctcccatg
     1201 ccgagccaga tgaggtcgaa gcgcgggatg ccctcaccac cgatcacctc gcgaagctcg
     1261 cgctcgtact gccaggcgcc cgcatccagg tcctggagct cgcacggcat gcgatgcacc
     1321 tggtccatgc gcagtcctgg caggtagtcg agcagcgcca ggcgggcggt gttgtagttc
     1381 gactcggggt cattgggcgg cacggcgcgc tcgtcgccga agaagaactc cacccgcgac
     1441 caatccagca tccggacgcg cggcggcacc accagcagcg ggcacacggc gagcggcgtg
     1501 gttccgccgg tcagagccag gatgaagcgc cctcggcgcc ggatggcgtt gcggccggcc
     1561 ccgatcagcc ggtcggcggt ggccgccgcc accgactgca cgtcgggcag cacctcgaag
     1621 cggtgcgtca cgcggcgacc cccggctcgc gagcc
//
LOCUS       DATADN010000446         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1246547, whole genome shotgun sequence.
ACCESSION   DATADN010000446 DATADN010000000
VERSION     DATADN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246547"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..861)
                     /locus_tag="VHH53_05795"
     CDS             complement(85..861)
                     /locus_tag="VHH53_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2289701.1"
                     /translation="MIERAYRLGEGLGLPVWCQDEAGPYQAIPRLGASWQPTEQPGCL
                     PHEYIRGGTAKLLTLFRPATGEVRAEPVTAATNAVLHPWLMRELEAILTTCPPSPDPT
                     APGRHWSDWSYRTDPFGFNQKLPPIRMLLIWDNLAGHHTSSLVDWCHARGIWLLYTPV
                     AGSWLNMAESVQRILVRRSLDGQHPHDATQVMTWLAEAVVGWNAHPTPFVWGGKRAAR
                     RARARERRHALGGSGAYSRRPIGRHPRATTNTCKGYTHAN"
     gene            complement(858..1418)
                     /locus_tag="VHH53_05800"
     CDS             complement(858..1418)
                     /locus_tag="VHH53_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289702.1"
                     /translation="MTRRQKDSLRPLTDDERQTLDRVSRAGSERADRVARAKALLAVA
                     DGASFTAAARRAGRRSGDGVAQLVARFNRSGLSALDAQHGGGATTQYGVTERDRILRE
                     LQRPPDRDQDGTATWSLTTLQRALRRAPDGLPSVSTWTILTTLHEAGYTFQRDRTWCH
                     TGTATRPRKDGTRVAVIDPETTPKKS"
BASE COUNT          268 a          569 c          604 g          277 t
ORIGIN      
        1 ctactacggc tgccgcaacc ggaaccgtac cgagcgtgcg gcgcgctgcc cctcgcgcta
       61 tgtgcgggcc gcagaactag tgggtcagtt ggcatgcgta tagcctttgc aggtgttggt
      121 ggtggcgcgt ggatggcgtc cgatcgggcg gcggctgtag gcgccggagc cgccaagggc
      181 gtggcggcgc tcccgagcac gagcccggcg ggctgctcgt ttgccgcccc acacgaacgg
      241 ggtcgggtgg gcattccagc cgacgacggc ctccgccagc caggtcatca cctgtgtggc
      301 atcgtgcgga tgctgcccgt ccagcgaccg ccggacgagg atgcgctgca ccgactcggc
      361 catgttcagc cacgaccccg caacgggcgt atacagcagc caaatgccac gggcgtgaca
      421 ccagtccacc aacgacgagg tgtgatggcc ggcgaggttg tcccagatca acagcatccg
      481 gatcgggggc agcttctggt tgaagccgaa cggatctgtc cgatagctcc aatcagacca
      541 gtgccggccg ggtgccgtcg gatcagggct gggtgggcag gtagtgagga tcgcttccag
      601 ttcccgcatc agccagggat ggagcacggc gttggtcgcc gcggtgaccg gctcggcccg
      661 gacctccccg gtcgccggcc gaaacagggt cagcagctta gcggtgccgc cgcggatgta
      721 ttcgtgcggc aggcagccgg gctgttcagt gggctgccag gacgccccga ggcggggaat
      781 cgcctgatac ggcccggctt catcctgaca ccacaccggc aggcccagcc cctcgcccag
      841 ccggtaggcg cgttcgatca actctttttg ggggtggttt ccgggtcaat aacggccacg
      901 cgcgtgccgt ccttgcgggg gcgggtggcc gtaccggtgt ggcaccaggt gcgatcccgc
      961 tggaaagtgt atcccgcctc gtgcagggtc gtgaggatgg tccaggtgct cacgcttggc
     1021 aggccgtccg gtgcccggcg caacgcgcgt tggagggttg tcaaggacca cgtggccgtg
     1081 ccgtcctggt cccggtcggg tgggcgctgc aattcgcgca ggatccgatc gcgctccgtc
     1141 acgccatact gggtggtggc tccgccgccg tgctgggcat ccaacgccga caggccgctg
     1201 cgattgaagc gggcaacgag ctgggccacc ccgtcaccgg agcggcggcc ggcgcggcgg
     1261 gcggccgccg tgaaggaggc gccgtccgca accgccagca gcgccttggc acgagcgacg
     1321 cgatcggcac gctcactgcc tgcgcggctc acgcgatcca gcgtctgccg ctcgtcgtcg
     1381 gtgagagggc gcaacgaatc cttctgtcgt cgggtcatca tactgctccg aacatgcacc
     1441 tgggagggac acgagcagca gaacgcatcc ccgccataat ggcaaggggc acgtcaagtg
     1501 acccactagt tagattcttc cgagaccaca tgatcatcgc tgcggttgac gatgcccgag
     1561 atcacgccgg tccggtgacc gccggtcttc acgcacatgc ctacgccttc gcgccgagcg
     1621 agctagtccg gcggaggggt tcggcgcacg gtccggccgc ctatcctcaa cgggagcgat
     1681 ggccctgacg accggcagct cggtctcact ggccattg
//
LOCUS       DATADN010000447         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_657023, whole genome shotgun sequence.
ACCESSION   DATADN010000447 DATADN010000000
VERSION     DATADN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657023"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VHH53_05805"
     CDS             complement(<1..881)
                     /locus_tag="VHH53_05805"
                     /inference="COORDINATES: protein motif:HMM:NF016099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HEX2289703.1"
                     /translation="MALTGALPGAAPPYGAALVENAVRAPQYAQVRRRILRQLLESLI
                     YEGVLTAASHGDRHGVGGTDESGKPVVYRFTMGLRYGFDRVRLGAEPVTRLVSGRERE
                     AESLTRFLIETREQLGAEPERVVGFARELEETLLKDAVAQYVRVLRADVLAGAEYNAL
                     ESMVMDGHLYHPAYKSRLGFDLEDHLAWGPEFTPLIHPLWLAARRSVATVTVSDRHTE
                     GGLLRGQLGQSYDEFHDRIRRAGGDPAKYTLLPVHPWQWREVIAHSHADALRDGDLIV
                     LGEDPDAHWPQQSIRTLA"
     gene            complement(881..2626)
                     /locus_tag="VHH53_05810"
     CDS             complement(881..2626)
                     /locus_tag="VHH53_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HEX2289704.1"
                     /translation="MHSPPHLAISPARARTIAQRAAQQRLLNCFLRETGVHVAAANAD
                     APLRVPLLQSGAVLLGSLRYWSVLGHHAYGDEFWVQPDGSDTATPVGQRELVALLLTE
                     LDSVAARAADGPSDGRRRQRRQAELAAQIDNSVAHTAHYLQRERPPQLDLPDPWALTR
                     RAEQSLLFGHPFHPTPKSAEGFNAQDLADYAPELGACFTLHYFAVAPQLLREDRVSSA
                     PWVPAEVQEPAQQLLGMDRAGYALLPVHPWQAGYLVQRDAVRALVADGGLVPLGPLGN
                     EVYPTSSVRTVCDSGFATAWKLPLHVRITNFVRNNPAEHARRAADAGRLLEALRKQWS
                     YDGFEVLLETGYRTIDDTALAADFTVLYRENPFATREQAPQVVAGLLEDNADGTEPEL
                     LRYVRQATARPEGQLPADHVAVWLRRYLEISLLPLLAVFSAHGVSLEAHVQNSLLHLE
                     GGWPARFFLRDMEGTSVSRQRCDAGSRDSLPADSPVLYDDAETWLRLKYYVVTNHLGH
                     LVHVLARHAEVDEWRLWQVVRQLLSDRCGADRYAADLLCSPVLPAKANLIGRFAGRGE
                     RPLYVDIPNPMCEVT"
     gene            complement(2683..4512)
                     /locus_tag="VHH53_05815"
     CDS             complement(2683..4512)
                     /locus_tag="VHH53_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
                     /protein_id="HEX2289705.1"
                     /translation="MIRASRNEQPSELVMRDLVDTLIQENLWGFADAAQQCRPAALCT
                     ETVDADEQWCRIDVAGGWVCFRARVAGALQPRRFSRPPVWHGGAPGAQPRQLRPDELL
                     ALLVGDCSDLTHAVAGSADLRTAVQHAAVVLSRRVRLVDAAPRPGELLTAERLAATRN
                     RPFHPTARAAIGWTAGELARYGPLRQQPLGLSWVAVRRDRLRGGTGSGSHRLHELLLD
                     ETDRQRLAAAVDRADLRIEDVQPLPVHPWQLDHVLPIAYAAELAAGDVVPLARGLGRF
                     HPTASLRTLTTTRQARLHVKLALGVSTLGATRLLPPRYLDNGERAQRTMQHLIERDPA
                     LRGLVQVCDERSWCGWAHPSGADEFDDRPGQLAAQLRTYPAGVLDDPTRLVLPMAALA
                     AHEWHVLSPVLGPAAGVVEFFRGLAAAFCELGLGFLRYGVLPELHGQNVVVVLRDGAV
                     EGFVLRDHDTLRLYPEWMAAEGVADPGYRIAPGAAQSLCLESAEALLGYLQTLGFQVN
                     LYGIADALRRHYGISEQVLWAQLRGAVVDTLARLALPGRVADVVERALLHAQTWPSRL
                     VLGPLLHRGRSGGVSMPAGTGRVPNPLAPPSQLATSPAALMAT"
     gene            complement(4534..4686)
                     /locus_tag="VHH53_05820"
     CDS             complement(4534..4686)
                     /locus_tag="VHH53_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289706.1"
                     /translation="MAAISKTLPSLPRPCRIVTIFPDRGDRYLDLVYDDAWLAKLQHR
                     DLGTAE"
BASE COUNT          758 a         1695 c         1674 g          676 t
ORIGIN      
        1 gccagggtac gaatcgactg ctgcggccag tgcgcgtctg ggtcctcgcc gaggacaata
       61 agatcgccgt cgcgcagcgc gtcggcgtgg ctgtgggcga tgacctctcg ccattgccac
      121 gggtgcacgg gcaaaagcgt gtacttggcc gggtcaccgc cggcacggcg gatccggtcg
      181 tggaactcgt cgtaggactg gccgagctgc ccacgcagca acccgccctc ggtgtgccgg
      241 tcagagaccg tcaccgtggc gacgctgcgg cgggcggcca gccaaagcgg gtggatgagg
      301 ggcgtgaact ccggccccca ggccaggtga tcctccaggt cgaacccgag ccgagacttg
      361 taagccgggt ggtaaagatg tccgtccatc accatcgact cgagtgcgtt gtactcggcg
      421 ccggccagca cgtcggcccg cagcacccgc acgtactggg ccaccgcgtc cttcagcagc
      481 gtctcctcca gctcgcgggc gaacccgacc acgcgttcgg gctcggcgcc cagctgctcg
      541 cgggtctcga tgagaaaccg ggtcagcgac tcagcctccc gctctcgccc gctgaccagc
      601 cgcgtcaccg gttcggcgcc cagccggacc cggtcgaagc cgtagcgcag acccatcgtg
      661 aaccggtaca cgaccggttt tcccgactcg tcggtcccgc caaccccgtg gcggtcgccg
      721 tgggaggctg cggtgagcac gccctcgtaa atcaacgatt cgaggagctg acgcaggatc
      781 cgccgccgga cctgggcgta ctggggcgcc cggaccgcgt tctcaaccag ggccgcccca
      841 tacgggggcg cggccccggg cagcgcgccg gttagggcca tcacgtcacc tcacacatcg
      901 ggttggggat gtccacataa agtggccgct cgccgcgtcc ggcgaagcga ccgatcaggt
      961 tggccttggc cggcagcacc ggcgagcaca gcaagtcggc ggcataccgg tcggctccac
     1021 accggtcgga cagcagctgc cgcaccacct gccacaaccg ccactcgtcg acctcggcgt
     1081 gccgggccag gacatgcacg agatgaccga ggtgattggt gacgacgtaa tacttcaggc
     1141 gcagccacgt ctcggcgtcg tcgtagagca ctgggctgtc ggcaggcaac gagtcgcggc
     1201 tacccgcgtc gcagcgctgg cggctcacgc tggtgccctc catgtcgcgc aagaagaacc
     1261 gggccggcca gccgccttcg aggtgcagca acgagttctg cacgtgagcc tccaggctca
     1321 cgccgtgtgc ggagaacacc gccagcagcg gcagcaggga gatttccaga tagcgacgca
     1381 accacaccgc gacgtgatcg gccggcagct gtccctccgg gcgcgcggtg gcttgcctga
     1441 cgtaccggag cagctctggt tccgtcccgt ccgcgttgtc ctccagcagg cccgcgacga
     1501 cctgaggtgc ctgctcgcga gtggcgaagg gattctcccg gtacagcacg gtgaagtcgg
     1561 cggccagcgc ggtgtcgtcg atcgtgcgat acccggtctc cagcaacact tcgaagccgt
     1621 cgtaggacca ctgcttgcgc agggcctcga gcagccggcc ggcgtcggcg gcccgccgtg
     1681 cgtgttcagc tgggttgttt cggacgaagt tggtgatccg cacgtgcagc ggcagcttcc
     1741 acgcagtggc aaaaccggag tcgcacaccg tacgcaccga cgaggtcggg tacacctcgt
     1801 tgccgagcgg acccaggggc accaggccac cgtcggccac cagggcccga acagcgtcgc
     1861 gctgcaccag gtaaccagcc tgccacgggt gcaccggcag cagcgcgtaa ccggctcgat
     1921 ccatgcccag cagttgctgc gccggctcct ggacctcagc tggcacccag ggggcggacg
     1981 acacccggtc ctcgcggagg agttggggag cgactgcgaa atagtgcagg gtgaagcagg
     2041 cccctagctc gggcgcgtag tccgcgaggt cctgtgcgtt gaatccctca gcgcttttgg
     2101 gcgtggggtg gaagggatga ccgaacagca acgactgctc ggcgcgccgg gtcagcgccc
     2161 agggatccgg gaggtccagt tgcggcggac gttcccgttg caggtagtgg gccgtgtggg
     2221 ccacgctgtt gtcgatctgg gcggccaatt cggcctgccg tctctgtctg cggcgtccat
     2281 cggatgggcc gtcggctgct cgcgcggcga cgctgtccag ctcggtgagc agcagcgcca
     2341 ccagctcccg ctgacccacg ggcgtggcgg tgtcactgcc atcgggttgc acccagaact
     2401 catcaccgta ggcgtggtga ccaagaaccg accagtaccg caggctgccc agcaagacag
     2461 cgccggactg gagcagtggc acccgcagcg gcgcgtcggc gttggctgcg gcgacgtgta
     2521 cgccggtctc ccgcaagaaa cagttgagca gccgctgctg agcagcccgc tgcgcgatcg
     2581 tgcgcgcccg cgccggactg atcgccaggt gcggcgggga atgcatgcta ggtcacctac
     2641 ttctgggcca ggagcgatgc gggccgtgct ctccggggcg catcaggtcg ccatcagcgc
     2701 cgcggggctg gtcgccaact gggacggggg cgccagcggg ttgggtaccc ggccggtacc
     2761 ggccggcatg ctcacaccgc cggaccgtcc gcgatgcagc agcggcccca gcaccaggcg
     2821 gctcggccac gtctgcgcgt gcaacagtgc acgttcgacc acgtcagcga cccgcccggg
     2881 cagcgcgaga cgggccagcg tgtcgacgac cgcaccccgg agctgcgccc acagcacctg
     2941 ctcgctgatg ccgtagtgcc ggcgcagcgc gtcagcgatg ccgtacaggt tgacctgaaa
     3001 ccctagcgtc tgcaggtatc ccagcagcgc ttcggcggac tccaggcaca gcgactgcgc
     3061 ggcacccggc gcgatccggt agccgggatc ggccacgccc tcggcggcca tccactccgg
     3121 gtagagccgc aaggtgtcgt ggtcgcgcag cacgaacccc tccaccgcgc cgtcgcgcag
     3181 caccaccacc acgttctggc cgtgcagctc gggcagcacc ccgtagcgca gaaagcccag
     3241 tccaagctcg cagaacgccg cggccaaccc gcggaagaac tccaccacgc cggcagctgg
     3301 accaagtacc gggctcagca cgtgccactc gtgcgctgcc agcgccgcca tcggcagcac
     3361 caacctggtc gggtcgtcga gcaccccggc cgggtaggtg cgcagctgcg ctgcgagctg
     3421 cccggggcgg tcgtcgaact catcggcgcc tgacgggtgc gcccagccgc accagctgcg
     3481 ctcgtcacag acctgcacga gcccgcgcag ggccgggtcc cgctcgatca ggtgttgcat
     3541 ggtgcgctgc gcgcgctctc cgttgtccag ataccggggt ggcagcagcc gggtggcacc
     3601 gagggtggac accccgagtg cgagcttgac gtgcagccgc gcctgccgtg tggtggtgag
     3661 ggtgcgcagc gaggcggtgg ggtggaaccg ccccagcccg cgggcgagtg gcaccacgtc
     3721 gccggccgcc agctcggccg cgtacgcgat gggcagcacg tggtcgagct gccacgggtg
     3781 taccggcagc ggctggacgt cctcgatacg caggtcggcg cgatcaacgg cggcggccag
     3841 gcgttggcgg tcggtctcgt cgagcaacag ctcgtgcagc cggtgcgacc cgctgccggt
     3901 accgccacgc agccggtccc ggcgtaccgc cacccagctc aagcccagtg gttgctgccg
     3961 caacggcccg taccgagcca gctcacccgc cgtccagcca atggcggcgc gggcggtcgg
     4021 gtgaaacggc cggttgcggg tggcggcgag ccgttccgcg gtgagcaact cgccggggcg
     4081 tggcgcagcg tcgaccaagc ggacccgcct ggagagcacc acggccgcgt gctgcaccgc
     4141 cgtgcgcagg tccgcgctgc ccgcaacagc atgggtcaaa tccgagcagt cgcccaccag
     4201 cagcgccaac aactcgtcgg gccgtagctg ccggggctgc gctcccggcg cgccaccgtg
     4261 ccagacgggc gggcgggaga accggcgcgg ctgcaaggct cccgccacgc gggcccggaa
     4321 acacacccat ccaccggcca cgtcgatgcg gcaccactgc tcgtcagcgt ccacggtctc
     4381 ggtgcacagc gccgccggcc gacattgctg tgcggcgtct gcgaaccccc acaggttctc
     4441 ctggatcagt gtgtccacca gatcccgcat gacgagttcg gatggttgtt cgtttcggga
     4501 ggcacggatc acggctgctc cgtcagtgcg acgtcactcg gccgtgccga ggtcgcggtg
     4561 ctgcagcttg gccaaccatg cgtcgtcata gacgagatcg aggtagcgat ccccgcggtc
     4621 cgggaagatc gtgacgatgc ggcagggcct gggcaggctg ggcaacgtct tggagatcgc
     4681 ggccacgact gacccgctgg agccacccgc gaagatgccc tcggtgtcca ggacagcgcg
     4741 caggcctgcc gcgctgtcct ggagaccgag ggcatcttcg cgggtggctc caccgggtca
     4801 gtc
//
LOCUS       DATADN010000448         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_657065, whole genome shotgun sequence.
ACCESSION   DATADN010000448 DATADN010000000
VERSION     DATADN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657065"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VHH53_05825"
     CDS             <1..1149
                     /locus_tag="VHH53_05825"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2289707.1"
                     /translation="YVDDGRLPGWLVLASRRGRIAYLSTYGHRDIEAGLPVQPDTLFR
                     IYSMTKPITSVAAMMLYEQGAFQLTDPISWYLPEFADMRVFVTGTAAKPVTVPAKEPI
                     RVWHLLTQTAGLTYGFHQANPVDAMYRAAGFEFVASPGKDLAASCAVWATLPLLFEPG
                     TEWNYSISTDVLGRLVEVVGGKPLDDFFAERIFQPLAMTDTGFWVDRPHAGRLAALYT
                     PHPATGRAIRLDTYGNIALRRPETLSGGDGLVSSAGDYHRFARMLACGGELDGVRLLG
                     PRTVRYMIRNQLPGGADLETFGRSVSAEVRYEGVGFGLGFAVVQDPVAYRTLTSVGEF
                     SWGGLASTAFWVDPVEDLIVLFLTQLAPSSTYPIRTQLRQLVNQALVD"
     gene            1359..1889
                     /locus_tag="VHH53_05830"
     CDS             1359..1889
                     /locus_tag="VHH53_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289708.1"
                     /translation="MSGPGVARAVLSLLTTIGFMMFAAWMIFAGVAIGGGPVQQIAAT
                     PPVRALPAEPTAPGSTLAFDEVWTTSDGNTIIAGAPKMGTSDSSLHPGMSVIRVAVTL
                     TNNGDTEWSPVFTTFNGTLNRAPVQESNEGDWRYSMPIAPNTSVTLSKVFLGEGGQFS
                     LAVSTSDGVALFTGRV"
     gene            complement(1873..>2368)
                     /locus_tag="VHH53_05835"
     CDS             complement(1873..>2368)
                     /locus_tag="VHH53_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX2289709.1"
                     /translation="TDPARARDLLIVFAEFARYSVRSVAETTLAEELENIELYLTLQQ
                     ARFGERLQVQLHVAPDALPVVLPFLAVQQLVDNAVRNGIERKQLGGTVTVTAGMDGDD
                     CVITVEDDGPGTEDAALLANLRTIDDQLHASFGDDYDLVADTVPGSGTKISIRMPGRV
                     RPDR"
BASE COUNT          373 a          774 c          806 g          415 t
ORIGIN      
        1 tacgtcgacg acgggcggct gccgggctgg ctggtgctgg ccagccggcg cgggcggatc
       61 gcgtacctgt cgacctacgg gcatcgcgac atcgaagccg ggctgccggt gcagccggac
      121 accctgttcc gcatttactc gatgaccaag ccgatcacgt cggtggccgc gatgatgctc
      181 tacgagcagg gcgcgtttca gttgaccgac ccgatcagct ggtacctccc cgagttcgcc
      241 gacatgcgcg tcttcgtcac gggcaccgct gccaagccgg tcacggtgcc cgcgaaggag
      301 ccgatccggg tctggcacct gctcacccag accgcgggct tgacctacgg gttccaccaa
      361 gccaacccgg tggatgcgat gtaccgggcg gccgggttcg agttcgtcgc ctcacccgga
      421 aaggatctcg cggcgtcgtg tgcggtgtgg gccacgctgc cactgctgtt cgagccgggc
      481 accgaatgga attactcgat ctccaccgat gtgctgggcc ggctggtgga ggtggtaggc
      541 ggcaagccgc tggacgactt cttcgcggag cggatcttcc agccgctggc gatgaccgac
      601 accggcttct gggtggacag accccacgcc gggcggctcg ccgcgctcta caccccacac
      661 cccgctaccg gccgggccat ccggttggac acctacggca acatcgccct gcgcaggccg
      721 gagacgctgt ccggcggcga tggcctggtg tcctctgccg gcgactatca ccgcttcgcg
      781 cgaatgctgg cgtgcggcgg ggagctggac ggggtgcgac tgctcgggcc tcgcacggtc
      841 cgctacatga tccgaaacca acttcccggc ggcgccgacc tcgagacatt cgggcgctcg
      901 gtgtccgccg aggtccggta cgagggcgtg ggattcgggc tcggtttcgc ggtggtgcag
      961 gacccggtcg cctaccgcac gctcaccagc gtcggggagt tctcctgggg cgggttggcc
     1021 agcaccgcat tctgggtgga ccccgtcgag gacctgatcg tgcttttcct tactcagctg
     1081 gcgccgtcgt cgacctaccc gatccgaacc cagctgcgcc agctcgtcaa tcaggcgctg
     1141 gtggactgat ccacgctgta gattacaaat agtgacagat tcacccctta gagtcggtaa
     1201 cgagtcggtt ctgcgcagcg aaaactacag tgatgggtac gtgcggtaac cggcacgggg
     1261 ttgtagcggc agctgttcga ctcgctccag gggaggcctc tgtgttcgtg cctgcgccgc
     1321 cgtacggttc cttcgcgccg ccgagaccgt cgcatccgat gagtggccca ggtgtcgcgc
     1381 gcgccgtgct cagcctgctg accacgatcg gattcatgat gttcgccgcc tggatgatct
     1441 ttgccggggt ggccatcggt ggtggtccgg tgcagcagat cgccgctaca cctccggtgc
     1501 gtgcgctgcc cgccgaaccc accgcgccgg gctccacgtt ggccttcgac gaggtctgga
     1561 ccaccagcga tggcaacacg atcatcgcgg gggcaccgaa gatgggcacg tccgactcgt
     1621 cgctgcatcc gggcatgtcg gtgatccggg tcgcggtgac actgaccaac aacggcgaca
     1681 cggagtggag cccggtcttc accacgttca acggaacgct caaccgcgcg ccggtgcagg
     1741 aatcgaacga aggcgactgg agatactcga tgccgatcgc cccgaacacg tcggtcaccc
     1801 tcagcaaggt atttctcggt gagggcggtc agttcagcct ggccgtgagc acctcggacg
     1861 gcgtggcgtt gttcaccggt cgggtctgac ccggcccggc atccggatgg agatcttggt
     1921 accgctgccg gggacggtat cggcgaccag gtcgtagtcg tcgccgaagc tggcatgcag
     1981 ctggtcatcg atggtgcgca ggttggccaa cagggcagcg tcctcggtgc cgggaccgtc
     2041 gtcctcgacg gtgatcacac agtcgtcacc gtccatgccg gcggtgacgg tgacggtgcc
     2101 gccgagttgc ttgcgctcga tgccgttgcg gaccgcgttg tccaccagct gctggacggc
     2161 cagaaacggc agcacgaccg gcagcgcgtc cggggcgaca tgcagctgaa cctgaaggcg
     2221 ttcgccgaag cgagcctgct gcagggtcag atacagctcg atgttctcca gttcctcagc
     2281 cagggtggtc tcggcgaccg accggacgga gtaccgggcg aactcggcga aaacgattag
     2341 cagatcgcgc gcgcgggccg ggtcagtg
//
LOCUS       DATADN010000449         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_363537, whole genome shotgun sequence.
ACCESSION   DATADN010000449 DATADN010000000
VERSION     DATADN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363537"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VHH53_05840"
     CDS             complement(<1..214)
                     /locus_tag="VHH53_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289710.1"
                     /translation="MAEVPRAQLIHIDVDRRLGHEWDEWDGKPLPNQGNYDSPPALFF
                     AWSALTLVALLTVAAAGLYLLAPRIEA"
     gene            242..>1520
                     /locus_tag="VHH53_05845"
     CDS             242..>1520
                     /locus_tag="VHH53_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2289711.1"
                     /translation="MLPQPRIRGAYRTESRARAAYAEGAGIYRIIPEAVCLPADVADL
                     RTVVCWAADQRIHLIPRGAGSAMGGGNVGSGVVLDLTGMTDRRLEVDPGTRRAVTTAP
                     ITLTALNQAASQYGLRLPPDPSSGRWATLGGMVATNAAGARSVRFGSVRGWVEGLTLL
                     TADGELLRLRRGEPPNTEIQAARRFMQTAAPELRAARAEIAARFPRTRKNSSGYALDA
                     YQESGDLLDLVIGSEGTLGVVTEIEWRLDSIPLHRAAVRVALRSLDSLAGAVEAMLPC
                     MPSAVELLDRSFLDLVATDTPAGPGLRFPSVEAVLLVELERDDPDSLRDALETITARL
                     RPIAYSVDTAFSEKAAERLWALRHAASPMLASLPPSRRSLQVIEDACVPVTRMGEYVR
                     LVRQSAAARSIPVVMFGHAGDGHLHVNLLPDVDQ"
BASE COUNT          251 a          498 c          506 g          265 t
ORIGIN      
        1 ccgcctcgat ccggggtgcg agcaggtaca gtcccgctgc tgcgactgtg agaagcgcga
       61 ccagggtcag cgcggaccag gcgaagaaga gtgccggagg cgagtcgtag tttccctgat
      121 tggggagcgg cttgccgtcc cactcatccc actcgtgtcc cagccggcgg tctacatcga
      181 tgtggatcag ttgagcgcgg ggaacctcag ccatagatca caagatagtt agcttaacgc
      241 catgctgcct caaccccgga ttcgtggtgc gtatcgcacc gaatccagag cccgcgcggc
      301 ctacgcagag ggtgcgggga tctatcggat tattccggag gccgtgtgcc tgcccgccga
      361 cgttgcggac ctcaggacgg tagtctgctg ggcagccgat cagcggatcc acctgatccc
      421 tcgaggagcg ggcagtgcca tgggcggagg caatgtgggc agcggcgtcg tcctcgacct
      481 gaccggaatg accgatcgtc ggctcgaggt cgacccgggc acaagacggg cggtgaccac
      541 cgcgccgatc actctgactg cactgaacca ggccgccagc caatacggac tgcgactgcc
      601 gccagaccca tcgagcggcc gctgggccac actggggggc atggtggcga ccaatgccgc
      661 aggagccagg tccgtgcgat tcggcagcgt ccgcggttgg gtcgaagggc tcacgctgct
      721 taccgcagat ggcgagctgc ttcggctcag gcggggtgag ccaccgaaca ctgagatcca
      781 ggcggcgcgg cgtttcatgc agacggctgc cccggagctt cgcgccgcgc gggcggagat
      841 cgccgctcgc ttcccccgga cccgaaaaaa ctcctccggc tatgcgctcg atgcctacca
      901 ggagtccggc gatctgctcg atctggtcat cggctccgaa ggaaccctcg gcgtggtcac
      961 agagatcgaa tggaggctcg actccattcc cttacatcgt gcggcggtga gggtggctct
     1021 tcgttcgctg gattccctgg ccggcgcggt cgaggcaatg ctcccgtgta tgccctcagc
     1081 agtcgagctg ctggatcgca gcttccttga tctggtggcc accgacacgc cagccgggcc
     1141 cgggctgcgc tttccttcgg tggaggcggt gttgctggtt gagctggagc gcgacgaccc
     1201 cgactcgctg cgagacgcac tggagaccat taccgcacgc ttgcgcccga tcgcgtactc
     1261 agtggatacg gcgttctccg aaaaggcggc cgaacggctg tgggcgcttc gccacgcggc
     1321 aagcccgatg ctcgcgtcgc ttcctcccag ccgccgctcg ctgcaggtca tcgaggacgc
     1381 ctgtgtgccg gtgaccagaa tgggcgaata cgtccgcctg gtccggcagt cggctgctgc
     1441 tcgaagcatt ccggtcgtca tgttcggcca cgccggtgac ggacacctcc acgtcaatct
     1501 gctccccgac gttgaccagc
//
LOCUS       DATADN010000450         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_363829, whole genome shotgun sequence.
ACCESSION   DATADN010000450 DATADN010000000
VERSION     DATADN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363829"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VHH53_05850"
     CDS             <1..458
                     /locus_tag="VHH53_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX2289712.1"
                     /translation="LTVDNGASGASDGHVDTVSVIDMEASPPRVIDKVVVGDAPEGLA
                     ISPDGKTAIAVMLKGSAAIPKNAWFRHDRGSVVALRIDGKKVTRGEEVKVGRLPEGAV
                     FSADGNRLYVGNYVDSTVSILKLEGGRISDTGRVLALPGRPAAMRASRP"
     gene            complement(466..1047)
                     /locus_tag="VHH53_05855"
     CDS             complement(466..1047)
                     /locus_tag="VHH53_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2289713.1"
                     /translation="MARGRAGWALGFADEVWFSRLAQPALHAWMPQGERLRLIMHAAD
                     RDDPEAKALACYGLWLPERERMLLRFVAGRPVSAMTCTFLAWAAECLAAEGVRVLALI
                     WDNAPWHVSREVRTWIRNHNRQVKAAGVGCRLLVCRLPSRSPWLNPIEPRWAHGKRAV
                     VEPERKLTSEELQQRLCHLYRCQLLPPLAQKVD"
     gene            complement(1053..1571)
                     /locus_tag="VHH53_05860"
     CDS             complement(1053..1571)
                     /locus_tag="VHH53_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2289714.1"
                     /translation="MHAPIFVREPSAEERARLEAGLRAADAFTVRRAQIVLLSAEGRR
                     PREIARGLRCAVQTVRNGIRAFNAQGVASLVAASSRPRSAAPVLGAAELEGLRAILHR
                     SPRTFGHARSSWTLALLAEVAQAEGLSPSVLSIETIRQALLRLEVGWKRAKRWLTSPD
                     PAYAQKNAAATG"
BASE COUNT          286 a          569 c          598 g          235 t
ORIGIN      
        1 ccctcaccgt cgataacgga gccagtggtg cctcggacgg ccatgtcgac accgtcagcg
       61 tcatcgacat ggaggcgagc ccgccgcgcg tgatcgacaa ggtggtggtt ggcgacgctc
      121 cggagggctt ggccatcagc ccggatggga agactgccat cgccgtgatg ctcaagggca
      181 gtgccgcaat ccccaagaat gcctggttcc ggcacgaccg aggcagcgtg gtggcgctca
      241 ggatcgacgg caaaaaggtg acgcggggcg aggaggtgaa ggtgggtcgc ctgcccgagg
      301 gtgcggtgtt cagcgcggac gggaaccggc tgtacgtcgg caactacgtc gacagcaccg
      361 tctcgatcct caagctcgag ggcggcagga tcagcgacac cggccgtgtc ctggcgctac
      421 ccggccgccc ggccgcgatg cgcgccagcc gcccctagtg cagcctcaat ccaccttctg
      481 tgcgagcggc ggcaggagtt ggcagcggta aaggtgacag agccgctgct ggagttcctc
      541 gctggtgagc ttgcgctcgg gctcgaccac ggcccgcttg ccgtgcgccc atcggggctc
      601 gatcgggttc agccacgggc ttctgctcgg caggcggcag accaggagac ggcagccgac
      661 gccagccgcc ttgacctgcc gattgtggtt cctgatccag gtgcggacct cgcggctgac
      721 atgccaaggg gcattgtccc agatcagcgc cagaacccgg acgccttcag ccgcgaggca
      781 ttcggccgcc caggcgagga aggtgcaggt catggcgctg accggccggc cggcgacgaa
      841 ccgcaacagc atccgctcgc gctcgggcag ccacagccca tagcaggcga gcgccttggc
      901 ctcggggtca tcgcggtcgg ccgcgtgcat gatcaggcgc agccgttcgc cttgcggcat
      961 ccaggcgtgc agggccggtt gtgccaagcg ggagaaccag acctcgtccg caaagcccag
     1021 agcccagccc gcccggcctc gtgccaagcc gatcagccgg tcgcggcggc gtttttttgc
     1081 gcgtaggcgg gatcggggct ggtgagccag cgcttggccc gcttccagcc gacctccagg
     1141 cggagcagcg cctggcggat ggtctcgatc gacagcacgg aggggctgag cccttccgcc
     1201 tgcgccacct ccgccaggag agccagcgtc cagctgctcc tggcatggcc aaaggtgcgg
     1261 ggtgaccggt gcaggatcgc gcgcagccct tccagctcgg ccgcaccaag gacgggtgcc
     1321 gcactcctcg gccgcgacga cgcggctacg aggctggcca cgccttgggc gttgaaggcg
     1381 cggatgccgt tccgcaccgt ctggacagcg caacgcaaac cccgggcaat ctcccgcggc
     1441 cggcgcccct cagcgctcag gagcacgatc tgcgcgcgcc gcacggtgaa ggcatcggcg
     1501 gcacgcaagc ccgcttcgag ccgggcacgc tcctcagcgc tcggctcgcg gacgaagatg
     1561 ggtgcatgca tgcccggcag atgcgaagcc ccacccgctc gcacaagggg ttggatgaag
     1621 atgcactagt gacgagcagg gcaagaggaa acagggtcag gccgagtgca cgcatgatgg
     1681 acatggaa
//
LOCUS       DATADN010000451         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_796817, whole genome shotgun sequence.
ACCESSION   DATADN010000451 DATADN010000000
VERSION     DATADN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796817"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VHH53_05865"
     CDS             <1..400
                     /locus_tag="VHH53_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289715.1"
                     /translation="PAPTPRAMEWEPGSAGEAGEGDSRQMGLGDLLAEALAAYRESRD
                     VRADAAALAGPHGEDGSAAGDIASGRDSPAYRGPGTRWASGLQLAPPIDRREDDPSEA
                     LTNPLLRLPDLTAEPLWVPPETGRRSAAGD"
     gene            496..921
                     /locus_tag="VHH53_05870"
     CDS             496..921
                     /locus_tag="VHH53_05870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX2289716.1"
                     /translation="MTVPAFSTDVAVRWSDMDFYGHVNNARVVTLLEEARTELLFGEG
                     ARRGAEELTHGVVVVKLEVRYRQPLVYSATPVRVRLWISELRAASFAVDYVVSSADGD
                     AVTARTWLAPYDTKAKRPRRLMPVEREFLAAFREGGHDG"
     gene            914..1546
                     /locus_tag="VHH53_05875"
     CDS             914..1546
                     /locus_tag="VHH53_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289717.1"
                     /translation="MAELRIPDPVERDDLGAFVARAVRLESDAIVRLRNGDGRVHAWV
                     VTPFDTLVTRSVRGAMLPGDLTVRGSNLLAAVTVVDAAVVDPGKPQDELWRSALPPEQ
                     GWHEVDQVPAEVLADLADQGAAVARDNPGPRGAPPAALLDQKVLTVSGAGFDVKVPLR
                     CLFALSGMGFVGGHAAAETVRVSATDSWLRLDARFGAVLRRRHALLPLLV"
     gene            complement(1550..>2042)
                     /locus_tag="VHH53_05880"
     CDS             complement(1550..>2042)
                     /locus_tag="VHH53_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX2289718.1"
                     /translation="ELGLPNVDLPDWALQDPVWQRSGRTERGRDGCRVPLPWDGDAPP
                     YGFTSTPGAWLPMPSGWADRTVAAQLEDPASTLSLYRQALQLRREHQTFAGTELLWYG
                     APPGCFAFLRKEGGLVCALNASEVYVPLPNGQLLLASGPLSPGGDALPPDTAVWFVEP
                     SPS"
BASE COUNT          310 a          673 c          732 g          327 t
ORIGIN      
        1 gccggcccca acaccccggg cgatggaatg ggagcctggg agtgcaggcg aagccggcga
       61 aggagactcc cggcagatgg gtctcggcga cttgctggcc gaggcgctag ccgcatatcg
      121 ggaaagccgt gatgtccggg ctgacgcagc cgccctggcc ggaccgcacg gcgaggacgg
      181 ctccgccgcc ggcgatatcg cgagtggtcg cgactcacct gcataccgcg gtccaggcac
      241 gcgctgggca tccggtcttc agctcgctcc accgatcgac cggcgcgaag acgatccctc
      301 cgaggcactt accaaccccc tgctgcggct gccggatctc accgctgaac cattgtgggt
      361 ccctcccgag acgggtcgcc ggtctgctgc cggtgactag cgtcctaccg cagatgtgat
      421 gtcgggcttg aacctggacg tcgcgaccgt gtcacgcgct gacccggctg ggagcaggtt
      481 gaccgaaggg tggatatgac agtgccggcg ttcagcacgg acgtggcggt gcgctggtct
      541 gatatggact tctacggaca tgtcaacaat gcgcgggtgg tgacgctgct ggaggaggca
      601 cgcaccgagc tgctgttcgg tgaaggcgcc cgacgtggtg ccgaggagct cacccacggt
      661 gtggtcgtcg tcaagctcga agtgcgctac cggcagccgc tggtgtactc ggctacgccg
      721 gtgcgggtgc gactgtggat cagcgagctg cgtgccgcct cgttcgcggt ggactacgtg
      781 gtgtcaagtg cggacggtga cgccgtcacc gcgcgaacct ggctggcacc ctacgacacc
      841 aaagcgaaac ggccacgccg gcttatgccc gtggagcgag agttcctggc tgcgtttcga
      901 gagggcggcc acgatggctg agttgcggat tccggaccct gtcgagcggg acgatctcgg
      961 cgccttcgtc gcccgcgcgg tccgcctcga gagcgacgcg atcgttcggc tgcgcaacgg
     1021 ggacggcagg gtgcatgctt gggtggtcac cccgttcgac acgctggtga ctcgttcggt
     1081 gcgaggggcc atgcttcccg gtgatctgac cgtgcggggt tcgaacctgc tggccgccgt
     1141 gacggtggtc gacgctgcgg tggtcgatcc cggtaagcct caggacgagc tgtggcgttc
     1201 ggcgctgcct ccggaacagg gctggcatga agtcgaccag gtgcccgccg aggtgctcgc
     1261 cgatctcgcc gaccagggcg ccgctgttgc cagggacaat cccggcccgc gcggcgcccc
     1321 gcctgctgcg ctgctcgacc agaaggtgct caccgtcagc ggagccggat tcgacgtcaa
     1381 ggtaccgctg cgctgcctgt tcgccctgtc cgggatggga ttcgtaggcg gccatgccgc
     1441 cgccgagact gtccgggtgt cagccaccga ttcgtggctg cggctggatg ctcgcttcgg
     1501 tgcggttctg cgccgccggc acgccctgct tccactgctt gtgtgaccgt taggacggcg
     1561 aaggctcgac gaaccagacg gcggtatcgg gcgggagcgc atcgccgccc ggtgaaagcg
     1621 ggccgctggc cagcaacagc tgcccattcg gtagcggaac atatacctcg gaggcgttca
     1681 gagcacagac cagcccgccc tccttgcgca ggaaggcgaa acatccaggc ggtgcgccgt
     1741 accacagcag ctcggtgccg gcgaacgtct gatgctctcg gcgcagttgc agagcctgcc
     1801 ggtacagcga cagcgtcgag gcggggtcct ccaactgggc cgccacggta cggtcggccc
     1861 agccgctggg catcggcaac caggcgccgg gggtggaggt gaacccgtaa ggcggagcat
     1921 cgccgtccca cggcagcggc acccggcagc cgtcgcgccc gcgctcggtg cgcccagagc
     1981 gctgccacac cgggtcctgt agcgcccagt caggcaggtc gacgttcggc aggcccagct
     2041 cg
//
LOCUS       DATADN010000452         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_510189, whole genome shotgun sequence.
ACCESSION   DATADN010000452 DATADN010000000
VERSION     DATADN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510189"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VHH53_05885"
     CDS             <1..252
                     /locus_tag="VHH53_05885"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984034.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEX2289719.1"
                     /translation="EVGTLDELDEALAAEVSLVLLDNMTVAQCAEAVRRAEGSGTELE
                     ASGRLTLDVASAYAATGVRYLAVGALTHSAPALDLGLDL"
     gene            complement(337..1341)
                     /locus_tag="VHH53_05890"
     CDS             complement(337..1341)
                     /locus_tag="VHH53_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2289720.1"
                     /translation="MSLPSRWVRRVVLAPAMVLLTVLVLTTLPVWMLLAAALSPFLPG
                     RLRALRVLWVAVVALVLESVALVALLGLWLAAGFGLAIRTPPFQYLHYQLVGWYLRVL
                     YREAARVLCLRVDIEGPGPDDYPDRPLLVFCRHAGPGDSFLLVHALVNWYAREPRIVL
                     SEALQWDPGLDVVLNRLPNRFLPDGGGEQLQQRIGELASSLDHNDAFVIFPEGGNFTE
                     RRRLAGIARLRARGLEDMARRSEAMRHVLPPRPGGVAAALAAAADADVVWVAHAGLDH
                     LYTMVDIWRELPLDGTVRMRWWRVPAAEVPRGEDAQVEWLYHWWERIDDWVDDTRRSP
                     "
     gene            complement(1338..>1971)
                     /locus_tag="VHH53_05895"
     CDS             complement(1338..>1971)
                     /locus_tag="VHH53_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2289721.1"
                     /translation="FAAGTVQRLHELARSGVAAHSSAPLHRAVTTVLGDRRIEDLPVR
                     FACCAARIEDAAEHWFDDGPIVDAVMASAAVPGLLPPAVVDGQHYLDGGLVNSIPLGR
                     AVELGADRIFVLHVGRVDQPLRPPRRPWEVALVAFEIARRHRYARDLASVGDDVEVHV
                     LPAGEGGAPRWDSRAALRYRDAAAASNRITAAHRASVKYLRERLGTGGRR"
BASE COUNT          336 a          719 c          658 g          258 t
ORIGIN      
        1 gaggttggca ccctcgacga gctggacgag gcactggcgg ctgaggtatc gctggtgctg
       61 ctggacaaca tgacggtcgc gcagtgcgcc gaggcggttc gccgcgccga aggctcgggc
      121 acggagctgg aggcttcggg ccgccttacc ttggacgttg cctcagccta tgccgcaacc
      181 ggggtccgct acctcgctgt gggtgccctc acccactccg ccccagccct agacctcggt
      241 ctggacctgt aggcgtccca tatagccgcc attcggagcc gaatcgcggt cccgggcccc
      301 tcaccattgc ttcgaaaggc gcctggcgag cagggttcag ggtgagcgcc gcgtgtcgtc
      361 cacccagtcg tcgatgcgtt cccaccagtg gtagagccat tccacttgag cgtcttcgcc
      421 acgcggtacc tcggcggccg gcacccgcca ccagcgcatc cgtacggtgc cgtccagcgg
      481 cagctcccgc cagatgtcga ccatggtgta caggtggtcc agccccgcgt gggccaccca
      541 caccacgtcg gcatcggcgg cggcggccag tgcggcagcg actccgcccg ggcgcggtgg
      601 cagcacgtgg cgcatcgcct cggaccggcg ggccatgtcc tcgagtcccc gtgcacgcaa
      661 ccgggcgatt ccggcaagcc ggcgacgttc ggtgaagttg ccgccctcgg gaaagatgac
      721 gaatgcgtcg ttgtggtcca gggagctcgc gagctcgccg atccgttgtt gcagttgctc
      781 accacccccg tcgggcagga atcgattggg cagccggttg agcaccacgt ccagccccgg
      841 gtcccactgc aacgcctcgg acagcacgat ccgcggttcc cgtgcgtacc agttcaccag
      901 cgcgtggacg agcaggaatg agtcgccggg accagcgtgg cggcagaaca cgagcaacgg
      961 ccggtcgggg tagtcatcag ggccgggacc ctcgatgtcc acccgcagac acagcacccg
     1021 ggccgcctcc cggtagagca cccgcaggta ccagcccaca agctggtagt gcaggtactg
     1081 gaacgggggc gtgcgaattg ccagcccgaa cccggcggcc agccagagtc cgagcaatgc
     1141 caccagcgcc acgctttcca gcaccagagc gacgacggcc acccacagga cgcgcaacgc
     1201 gcgcaggcga cccggtagga acggcgacag cgcggcggcc agcagcatcc ataccggcag
     1261 cgtggtgagc accaagacgg tgagcaacac catggcaggc gccagcacca cccggcggac
     1321 ccaccgggac gggagactca tcgccgaccc ccggtgccca gacgttcccg taggtacttg
     1381 accgacgcac ggtgcgcggc ggtgatccgg ttgctggccg cggcggcgtc gcggtagcgc
     1441 agcgccgccc ggctgtccca gcgcggcgcg ccgccctcgc cggccggcag cacgtgcacc
     1501 tcaacatcat cgccgaccga agccagatcg cgggcatagc ggtgccgccg ggcgatctcg
     1561 aaggccacca gagctacctc ccaaggccgc cgcggcggtc gtaacggctg gtcgacgcgc
     1621 ccgacgtgca gcacaaagat ccgatcggca cccagctcga cggcgcggcc cagcgggata
     1681 ctgttgacca ggccaccgtc gaggtaatgc tggccgtcga caaccgcggg tggcaacagc
     1741 ccgggcaccg ctgccgaagc catgacggca tcgacgatgg ggccatcatc gaaccagtgc
     1801 tccgcggcat cctcaattct tgcggcgcag cacgcaaagc gcaccggcag gtcctcgatg
     1861 cgccggtcgc ccagcacggt ggtcaccgcc ctgtgcagcg gagccgacga gtgcgcggcc
     1921 accccgctgc gagcgagctc atgcagccgc tggaccgtcc ccgccgcgaa c
//
LOCUS       DATADN010000453         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_657546, whole genome shotgun sequence.
ACCESSION   DATADN010000453 DATADN010000000
VERSION     DATADN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657546"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1085
                     /locus_tag="VHH53_05900"
     CDS             15..1085
                     /locus_tag="VHH53_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX2289722.1"
                     /translation="MRRIAGGTFERLTDHPAHALVGVLRIPESRQGPGASIGRRVVGA
                     ALALTVTVLLVYLGRDGYRDVNEDGLSLLDCIYYATVSLSTTGYGDITPVSESARLAN
                     VLVITPLRVLFLIVLVGTTLEVLTERSRQALKIQRWRRKVRDHVVVVGYGTKGRSAVS
                     SMLGDEAEPTAVVVVDTNPSALEFANARGLVTVNGNGTRSDVLRLAGVTRARAVVVAT
                     SRDDAAVLVTLTARELAPKAHIVAAVRERENVHLLRQSGANSVVVSSETAGRLLGMAT
                     KTPAVVDLVEDLLTPEAGLAIAQRTVEPDEVGGSPRHLPDIVLGVVRTGRLYRVDAPE
                     IDTVERDDILLYVRHVTQAESG"
     gene            complement(1305..1541)
                     /locus_tag="VHH53_05905"
     CDS             complement(1305..1541)
                     /locus_tag="VHH53_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289723.1"
                     /translation="MTADPRLQELAQRTPNFGFLLEHEAVLVGYGAAAESMVFTDANT
                     SMIKARQFDEQLVTVLIATFGIQGPQGSGHSTGV"
BASE COUNT          273 a          534 c          550 g          294 t
ORIGIN      
        1 ggcgcgctgc gttcatgagg cgaatagccg ggggcacctt cgagcgcctc accgatcatc
       61 cggctcacgc gctggtgggc gtcctccgga tcccggagag ccggcaaggc ccgggagcgt
      121 cgatcgggcg ccgggtcgtg ggcgccgcct tggcgctcac cgtcacggtg ctgctcgtct
      181 acctcggccg ggacggctac cgcgatgtca acgaagacgg cctttccttg ctggactgca
      241 tctactacgc cacagtgtcg ctttccacga ccggatacgg cgacattacc ccggtcagcg
      301 agtccgctcg gttggcgaac gttttggtga ttactccgct gcgggtgctg ttccttatcg
      361 tgctggtcgg gaccaccctg gaggtcctca ctgagcggtc ccggcaggcg ctgaaaatcc
      421 aacgctggag gcgcaaggtg cgcgatcacg tcgtcgtcgt tggttacggc acaaagggcc
      481 gttccgcggt gagctcgatg cttggtgacg aggccgagcc caccgctgtg gtcgtcgtcg
      541 acaccaaccc cagcgcgctg gagttcgcca acgcgcgtgg cctagttacg gtgaatggca
      601 acggcacccg ctccgacgtg ctgcgtttgg cgggagtgac tcgggcccgt gccgtggtgg
      661 ttgccaccag tcgagacgac gccgcggtgc tggtcacgct caccgcccga gaactggcgc
      721 cgaaagcgca tatcgtggca gcggtgcgcg agcgcgagaa cgtgcatctg ctgcgacagt
      781 ccggcgccaa ctccgttgtg gtgtcctccg agaccgcggg ccgcctgctc ggcatggcaa
      841 ccaagacacc tgcagtagta gacctggtcg aggacctgct caccccggag gccgggctgg
      901 ccatcgccca acgaacagtg gagcccgacg aggtcggtgg ttcgcccagg catttgcccg
      961 acattgtgct cggggtggtc cggaccggtc gactgtaccg ggtagacgct cccgagatcg
     1021 acacggtgga gagggacgac atcctgctct atgtccgcca tgtcacccaa gctgagtccg
     1081 gctgaaggcc aacggctcgt ccactcagga ccagccacgg cctgactgtc ctgcctgatg
     1141 gacgcgcgca atgctgctcg tcgggccgga gaacactgtg gcctagcggc gtacacatga
     1201 tgggcctgct gacgaccgtc gaggtacccg tgacgttgtc gagcgggccc gtacgcttgg
     1261 tttaccggtg acagtggtgt ggcccaccga gcagagcagc actttcagac gcctgtgctg
     1321 tgtccgctgc cctgcgggcc ctgaatgccg aaagtggcga tcagcacggt gaccagctgt
     1381 tcatcgaatt gacgggcctt gatcatcgac gtgttggcgt cggtgaagac catcgattcg
     1441 gccgcagcgc cgtatccgac cagcacggcc tcgtgctcga gcaggaaacc gaagttgggc
     1501 gtgcgctgcg ccaactcctg cagacgcggg tcggccgtca ccgctcaccg tccgtacccg
     1561 gcaagaaccg tgacgttaac agggggagcg gtggcagaac gggctcacgt ccagcccgac
     1621 agtcgatcat gcagcttccg cctattcagt c
//
LOCUS       DATADN010000454         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_38538, whole genome shotgun sequence.
ACCESSION   DATADN010000454 DATADN010000000
VERSION     DATADN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38538"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2952)
                     /gene="leuS"
                     /locus_tag="VHH53_05910"
     CDS             complement(64..2952)
                     /gene="leuS"
                     /locus_tag="VHH53_05910"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX2289724.1"
                     /translation="MNSTDAGDVPPYRYTAALAGEIECRWQRRWAQRGTFHAPNPVGA
                     LSSAEGIPVPDDKLFVQDFFPYPSGAGLHVGHPLGFIGTDVFARYHRMTGRNVLHTMG
                     FDAFGLPAEQYAAETGTHPRTTTEANIKRYLAQIRRLGLGHDERRRVATTDVEYYRWT
                     QWIFLQIYHAWYDTEAHKARPIAELQAEYAHGTRSTPDGRNWSELAPTEQREIIDSHR
                     LVYLSEAPVNWCPGLGTVLANEEITADGRSDRGNFPVFRRNLRQWMMRITAYADRLLD
                     DLDRLDWPDKIKTMQRNWIGRSHGARVRFPVQTAIGGQAGIEVFTTRPDTLFGATYMV
                     LAPEHPLVSAITAKTWESGTDPRWTAGAATPVEAVAAYRHSVSRRSDLERQESREKTG
                     VFTGAYATNPVNGQQIPVFVADYVLMGYGTGAIMAVPGHDQRDWEFAREFGLPIVEVI
                     AGGDIEQAAYVGTGAAVNSANDDVSLDGLAVEEASKAIIEWLNERGYGEGTVHYKLRD
                     WLFARQRYWGEPFPIVYAVGEDGEPDLGHPLPLPERFLPVELPDVVDYAPTTFDPDDP
                     HTEPAPPLAKARDWVEVELDLGDGPRRYVRDTNVMPQWAGSCWYQLRYIDPYNDDAMC
                     DPLNERYWMGPRPALHGGDDPGGLDLYVGGVEHAVLHLLYARFWHKVLHDLGHVSSEE
                     PYRRLFNQGYIQAYAYTDARGRYVPAEEVVESDGRYLYNSEPVTREYGKMGKSLRNVV
                     TPDEICEAYGADTFRAYEMSMGPLDASRPWATKDVVGLHRFLQRVWRNLVDENTGELH
                     VSCETPDELTLKAVHKAIAGVCEDYTTLRLNTAVAKLIELNNHVTKVYGGHAATPRQV
                     AEPLVLLLAPVCPHIAEELWERLGHEDSLAHGPFPEVDERYLVADTVEYPIQVNGKVR
                     ARVTVPASATLDDVRHAALTEPKVVELLEGAQPRKVIVVPGRLINLVI"
BASE COUNT          455 a         1018 c         1045 g          527 t
ORIGIN      
        1 agtactgcct actggagact tacccgatca gcccgcaact acaccggtgg cagccatctt
       61 ctcctagatg acgagattga tcaggcgacc ggggaccacg atgaccttgc gcggctgcgc
      121 cccctcgagc agttcgacga ccttgggttc ggtcagtgcg gcgtgacgca cgtcgtccag
      181 cgttgccgag gccggcacgg tgacccgggc ccgaaccttg ccgttgacct ggatcggata
      241 ctcgacggtg tccgcaacca ggtagcgctc gtcgacctcg gggaaaggcc cgtgagccag
      301 cgagtcctcg tgaccgagcc gttcccacag ctcctcggcg atgtgtgggc acaccggcgc
      361 gagcagcaac acgagcggct cggcgacctg gcgtggcgtg gcggcatgcc cgccgtaaac
      421 cttggtgacg tggttgttca gctcgatgag cttggcgacc gccgtattga ggcgcagcgt
      481 cgtgtagtcc tcgcagaccc cggcgatcgc cttgtgcacc gccttgagcg tgagttcgtc
      541 cggggtttcg cagctcacat gtagctcgcc ggtgttctcg tcgaccaggt tgcgccagac
      601 ccgctgcagg aaccggtgca gcccgacgac gtccttggtc gcccacggcc gggacgcatc
      661 cagcggtccc atggacatct cataggcgcg gaaggtgtcg gcgccgtagg cctcgcatat
      721 ctcgtccggg gtcaccacgt tgcgcaggct cttgcccatc ttcccgtact cgcgggtgac
      781 cggttcgctg ttgtagagat agcgcccgtc cgactcgacg acctcctcag cggggacgta
      841 gcgtccgcgc gcgtcggtgt aggcgtaggc ctggatgtaa ccctggttga acagccggcg
      901 gtagggctcc tcggagctga cgtgtcccag gtcgtgcagc accttgtgcc agaaccgggc
      961 gtagagcagg tgcaggaccg cgtgctcgac gccgccgacg tacaggtcca ggccgccggg
     1021 gtcgtcgccg ccgtgcagcg ccgggcgcgg gcccatccag tagcgctcgt tgagcggatc
     1081 acacatcgcg tcgtcgttgt acgggtcgat gtagcgcagc tggtaccagc aggatccggc
     1141 ccactgcggc atcacgttgg tgtcccggac gtagcgcctc gggccgtcac ccaagtcgag
     1201 ctcgacctcg acccagtcgc gggccttggc cagcggcggc gccggctcgg tgtgtgggtc
     1261 gtcggggtcg aaggtggtcg gcgcgtagtc gaccacgtcc ggcagctcga ccggcaggaa
     1321 ccgctccggc aacgggagcg ggtggcccaa gtcgggttcg ccgtcctcgc cgacggcgta
     1381 gacgatcggg aacggctcgc cccagtaccg ttgccgggcg aacagccagt cgcgcagctt
     1441 gtaatgcacg gtgccctcgc cgtatccgcg ctcgttgagc cattcgatga tcgccttgct
     1501 ggcctcttcg acggccaggc cgtccaggct cacgtcgtcg ttagcggagt tcaccgcggc
     1561 gccggtaccg acgtaggcgg cttgctcgat gtcaccaccc gcgatcacct cgacgatcgg
     1621 caggccaaac tcgcgcgcga actcccagtc acgctgatcg tgcccgggaa ccgccatgat
     1681 cgcgccagtg ccgtagccca tcaatacgta gtcggcgacg aagacaggaa tctgctgacc
     1741 attgacggga ttcgtggcat aggccccggt gaacacaccg gtcttctcgc ggctctcctg
     1801 gcgctccaga tcgctgcgcc gcgacacgct gtgccggtag gccgcgaccg cctccacggg
     1861 ggtggccgcg ccggccgtcc accgtggatc tgtgccggac tcccaggtct tggcggtgat
     1921 cgccgagacc agcgggtgtt cgggagccag caccatgtag gtcgctccga acagcgtgtc
     1981 ggggcgggtg gtgaacacct cgatgccggc ctgcccgccg atcgcggtct gcaccgggaa
     2041 ccggacccgg gcaccgtgcg agcgcccgat ccagttgcgc tgcatggtct tgatcttgtc
     2101 cggccagtcc agccggtcca ggtcgtcgag cagccgatcg gcgtaggcgg tgatccgcat
     2161 catccattgc cgcaggttgc gccggaacac cgggaagttg ccgcggtcgc tgcgaccgtc
     2221 ggcggtgatc tcctcgttgg ccagcacggt gcccaacccc gggcaccagt tcaccggcgc
     2281 ctcggacagg tagaccagcc ggtgcgagtc gatgatctcc cgctgctcgg tgggtgccag
     2341 ctccgaccaa ttgcgaccgt ccggggtgga ccgggtgccg tgggcgtact cggcttgcaa
     2401 ctcggcgatc gggcgggctt tatgcgcctc ggtgtcgtac cacgcgtggt aaatctgcag
     2461 gaatatccac tgggtccagc ggtagtactc gacgtcggtg gtcgcgaccc ggcggcgctc
     2521 gtcatggccc agccccagcc ggcggatctg ggccaggtag cgtttgatgt tggcctcggt
     2581 ggtggttcgc gggtgggtgc cggtctcggc cgcgtactgc tcagcgggca acccgaacgc
     2641 gtcgaagccc atggtgtgca gcacgttgcg cccggtcatc cggtggtagc gggcgaagac
     2701 gtcggtgccg atgaaaccca gtgggtgccc gacgtgcaac cctgccccgg acggatacgg
     2761 gaaaaagtcc tgcacgaaca gcttgtcgtc gggaactggg atgccctcag ccgagctcag
     2821 cgccccgact gggttgggcg catggaaggt tccccgctgc gcccagcgac gctgccagcg
     2881 acattcgatc tcgccggcca gcgccgcggt gtagcggtac ggcgggacgt ccccggcgtc
     2941 ggtgctgttc atgatcgtcc ttccgggccc ggccgtggtc atgtgatacc cccaggaaac
     3001 agcgaagccc cccaggcgct gaggccgtga ggggtcgccg cgctg
//
LOCUS       DATADN010000455         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_510303, whole genome shotgun sequence.
ACCESSION   DATADN010000455 DATADN010000000
VERSION     DATADN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510303"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VHH53_05915"
     CDS             complement(<1..228)
                     /locus_tag="VHH53_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289725.1"
                     /translation="MPETPAKSATWSWFGGSTEGGDTAEIPQTAETVDFETVDSGSRN
                     ADLGNVEAGNFAAYPDLAAQTEGTPRLPPWDD"
     gene            complement(308..628)
                     /locus_tag="VHH53_05920"
     CDS             complement(308..628)
                     /locus_tag="VHH53_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX2289726.1"
                     /translation="MSDSKLEIQMLHDRVMVRLVGEEGERRSSGGIVIPATAQVAKRL
                     SWGEVYGIGNHVRTVQVGDRVLFNPEDQFEFEVRGRAYLVVRERDLQAVASERTEHGT
                     GLYL"
     gene            complement(836..2032)
                     /locus_tag="VHH53_05925"
     CDS             complement(836..2032)
                     /locus_tag="VHH53_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2289727.1"
                     /translation="MDPVRNPFAPGAGQRPPELTGRDRELGAFEVVLERVARGRPERS
                     LVLTGLRGVGKTVLLGELRSMALRRGWGAGKVEARPDGGLRRPLSAALHRAVRDLALR
                     HRAPDRVEEVLGVLKAFALRAAPEGAKLRDRWQPGIDAPVIAGRADSGDIEIDLVELF
                     TEVAELAADVGTGVALLIDEMQDVAPDDVSALCAACHELSQSGAPLVVVGAGLPHLPA
                     VLSASKSYSERLFRYLRIDRLDRSDADRAVVAPIERESAAITPDALDKLFETSGGYPY
                     FVQAYGKAAWDAAPASPIGILDVQVAAPEAEGELAVGFFGSRFERATPAEREYLQSMA
                     ELTGGRDEPVGTTQVAGQLGRRASSLSPARDSLLKKGLVYSGERGQIAFTVPHFGRFL
                     LGRPAM"
     gene            2703..>3021
                     /locus_tag="VHH53_05930"
     CDS             2703..>3021
                     /locus_tag="VHH53_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289728.1"
                     /translation="MNVRWLSSNRALAASFIAVLLSVALLTVLVIRQHTESANGQVYL
                     ETAEGVGANPFVPLSPDTPEAVGGEVTPGGVTTPMSPGTDNVGTCDPGKLAAYLEANP
                     KKAS"
BASE COUNT          537 a         1026 c          957 g          501 t
ORIGIN      
        1 atcatcccac ggtgggagcc gtggtgtccc ttctgtttgt gctgcgaggt ccggataggc
       61 agcgaagttt cctgcctcaa cgttgcccaa gtcagcgttc cttgagccag aatctactgt
      121 ctcgaagtcc actgtctcag cggtctgcgg gatctcggcg gtgtcgccgc cttcggtcga
      181 cccgccaaac cacgaccatg tcgcggactt tgctggcgtc tcaggcaccg cacctccttc
      241 ccgtagacga gccggtcccg tagacgagcc ggcactccgg ctgccaccgg cctccgtcat
      301 gttgacgtca caagtagagg ccggttccat gttcggtgcg ctcgctggca acggcttgca
      361 gatcacgttc ccgcaccacc aggtaagcgc gcccgcgcac ctcgaactcg aactgatctt
      421 cggggttgaa cagcacccgg tcgcccacct ggacggtacg gacgtggtta ccgatcccgt
      481 acacctcacc ccaggacaac cgcttggcca cctgcgccgt cgccgggatg acgatgccgc
      541 cgctgctccg gcgctcgccc tcctcgccca cgaggcgcac catcacgcgg tcgtggagca
      601 tctggatttc gagcttgctg tcggacacgg cgacgagggt tgtgccccct tcggtgctgg
      661 ccggtgcggc ccgtggtgcc gccggcgtcg tgacatgggc atgacatagg aatgccccgt
      721 gaggctgccg ggtcgggggt ccaacagcat cacggggcta actgtgtcat cgctgtatgc
      781 ggtggcgccg ttacaagccc ttgccgacgg gtggttgttt cacccatctg ggggttcaca
      841 tggctggcct gccgagtagg aagcgcccga agtgcgggac ggtgaacgca atctgcccgc
      901 gttccccgga gtagaccagc cctttcttga gcaggctgtc ccgcgccggg gacagcgacg
      961 aggcccggcg cccgagctgg ccggccacct gggtggtgcc aaccggctcg tcgcggccac
     1021 cggtgagttc agccatcgac tgcaggtact cccgctcagc cggggtggcc cgttcgaacc
     1081 gggatccgaa gaatcccacg gcgagctcgc cttcggcctc gggagcggcc acctgcacgt
     1141 cgagaatccc gatcggcgac gccggggccg cgtcccacgc agccttgccg tatgcctgca
     1201 cgaagtaggg ataaccccct gaggtctcga acaacttgtc cagcgcgtcg ggagtgatcg
     1261 cggcactctc ccgctcgatc ggcgcgacga cagcgcggtc ggcgtcggag cggtccagcc
     1321 gatcgatccg caggtaacgg aacagccgct cggaatagga cttcgacgca ctgagcaccg
     1381 caggcaggtg cggcaacccc gctccgacca ccaccagcgg cgccccggac tgggacagct
     1441 cgtgacacgc agcgcacagt gcggagacgt cgtcgggcgc cacatcctgc atctcatcga
     1501 tcagcagcgc caccccggtg ccgacgtcgg cagccagctc ggcgacctcg gtgaaaagct
     1561 ccaccaggtc gatctcgata tccccggagt cggcgcggcc ggctatgacc ggtgcatcga
     1621 tgcccggctg ccagcggtcc cgcagcttgg cgccttccgg cgcggcgcgc agggcgaaag
     1681 ccttgagcac cccgagtact tcctcgaccc ggtccggggc ccgatggcgc agcgccagat
     1741 cacgcaccgc ccggtgcagt gccgccgaca acgggcggcg caacccaccg tcggggcgag
     1801 cctcgacctt gccggcgccc catccccggc gcagtgccat cgaccggagc tcaccgagta
     1861 gcaccgtctt gcccaccccc cgcaagccgg tcagcaccaa ggaacgttcc gggcggccgc
     1921 gggccactcg ctccagcacc acctcgaagg cgcccagctc ccggtcccgc ccggtcagct
     1981 cgggtggtcg ctggccggcg ccgggggcga acgggttacg caccggatcc atcctcggac
     2041 agtatccggc tgtctagcgt tggccgcaga ttccgcaata gatccctatt ggcgtgtcgc
     2101 gcccgggtgc gcggtgagtc ggcctgtggt ggcggcacgt gccattcaag accgaacgtg
     2161 cctggccatc gtctcgtctc acaacggcag gacgatcagc aagcagccaa cagccgcaat
     2221 gcgctgctcc gttcgggcta attttcaact ctgcgtagcg gcaccctaac gcagacgagc
     2281 ggttggacga gagaggaagc gacagccgac acagctgatc gcatcgacgg tgacgtggtg
     2341 agggcgtcac acaatgcacc cagccggagg tgtaagtcac agcttgttcg acatggggga
     2401 ccgttcatgc gccgcacaac ggcagtactc acacttcaga gagggcctga atgcctgagg
     2461 tgggagccga aacctggcga gacggaagtg tcgccgccgg aatcaactcc gttaacccac
     2521 gatacaggcc acgccggtgc tccttccggt aaatcatcgg gggcagacct cacgccgcca
     2581 ggtaagaccg ccatggacgc cttgagtgag gaggagacct ctgtgaaaag agcactccag
     2641 cgcgacacgg ctcggtggcg tcgtatcacc atggtcacgt cgcgcgaagg aaagattctt
     2701 cgatgaacgt tcgctggtta agttcgaaca gggccttggc ggcgtcgttt atcgccgttc
     2761 tgttgagcgt cgcattgttg actgtccttg tgatacgcca gcacaccgaa tcagccaatg
     2821 gccaggtcta tcttgagacg gccgagggcg tcggggcaaa cccgttcgtg ccactgtcgc
     2881 cggacactcc cgaagcagta ggcggagaag taaccccagg aggtgtgact actccgatgt
     2941 ctccaggaac tgacaacgtt ggaacctgcg atcccgggaa acttgccgcc taccttgagg
     3001 ctaatccaaa aaaggcgagc g
//
LOCUS       DATADN010000456         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_797075, whole genome shotgun sequence.
ACCESSION   DATADN010000456 DATADN010000000
VERSION     DATADN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797075"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1524
                     /locus_tag="VHH53_05935"
     CDS             244..1524
                     /locus_tag="VHH53_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2289729.1"
                     /translation="MSGVENAEDSKPATGLDGLDEQLVEQLVSRAKAGGLKLTGEGGV
                     LQQLTKRLLESALEGELADHLGYDKHDVAGRDGGNSRNGRRAKTVLTDVGPVEIEVPR
                     DRDGSFQLKIVAKRQKRLSGVDELVISLSAKGLTTGEVQAHLAEVYGAQVSRRTISTI
                     TDKVLEGMAEWQNRPLDPVYPVLFIDAINVKIRDGKVANRPIYVALAVTCDGRRDILG
                     LWAGDGGEGAKYWLHVLTEIKNRGVGDVCMVVCDGLPGLPDAVTATWPPAITQTCVVH
                     LLRNSFRYAGRQHFDAIAKALRPVYTAPTEAAAMERFLEFCETWGSRYPAIVRLWENA
                     WAEFVPFLAFDVEIRKVVCTTNAIESVNARIRRAVKARGHFPNEHAALKCVYLAIMSL
                     DPTGTGQRRWSMRWKPALNAFDIAFDGRLAAGRK"
BASE COUNT          268 a          530 c          545 g          260 t
ORIGIN      
        1 gtaatcgccg tccacgacca cgaacggcag gtcggatgtc gcgttcttcc accagaccag
       61 gcatccagcc cggtcattga tcttgacgtg gcgcagtcaa cagggtgctc cccgaactcc
      121 tgccattcgt cgtcggtggc cgggcggtgc tcctcctccg gccgcacggc gcggcgacgg
      181 gcggggactg taaaacgagc ggtgtaactc ccgatcatgg aagtgcacct gatgaccgac
      241 atgatgtccg gcgtggagaa cgctgaggac tcgaagcccg cgaccggtct ggacggcctg
      301 gacgagcagc tcgttgagca gttggtgagc cgcgccaagg ctggtgggct gaagctgact
      361 ggtgagggcg gggtgctcca gcagttgacc aagcggctgt tggagtccgc gctggagggc
      421 gagcttgccg accacctcgg ctacgacaag catgacgtgg ctggccgtga tggcggcaat
      481 tcccgcaacg gtcgccgggc caagacggtg ctgaccgatg tcggcccggt ggagatcgag
      541 gtgccgcgcg accgcgacgg cagtttccag ctcaagatcg tggccaaacg gcagaaacgg
      601 ctgtccgggg tggacgagct ggtcatctcg ctgtcggcga aggggctgac caccggcgag
      661 gtgcaggcgc atctggcgga ggtgtatggc gcccaggtgt cccgccggac catctccacg
      721 atcaccgaca aggtcctcga ggggatggcc gagtggcaaa accggccgct cgacccggtc
      781 tatccggtgc tgttcatcga cgcgatcaac gtgaagatcc gggacgggaa ggtggccaac
      841 cggccgatct acgttgcgct cgcggtcacg tgcgacgggc gccgcgacat tctcgggctg
      901 tgggccggcg acggcggtga gggcgccaag tactggctgc acgtgctcac cgagatcaag
      961 aaccgcggcg tgggcgacgt gtgcatggtc gtctgcgacg ggttgcccgg gctgcccgat
     1021 gcggtcaccg ccacctggcc cccggcgatc acccagacct gtgtggtgca cctgctgcgt
     1081 aacagcttcc gctacgccgg ccgccagcac ttcgacgcca tcgccaaggc gctgcgcccg
     1141 gtctacaccg cgccgaccga ggccgccgcg atggagcggt tcctcgagtt ctgcgagacc
     1201 tggggcagcc gctacccggc gatcgtgcgg ctgtgggaga acgcctgggc cgagttcgtc
     1261 cccttcctgg ccttcgacgt cgagatccgc aaggtggtct gtacgaccaa cgcgatcgag
     1321 tcggtcaacg cccggatccg ccgcgcggtc aaggcccgcg ggcacttccc caacgagcac
     1381 gccgccctca agtgcgtgta cctggcgatc atgagcctcg accccaccgg caccgggcaa
     1441 cgacgctggt cgatgcgctg gaagccggcc ctcaacgcct tcgacatcgc cttcgacggc
     1501 cgcctggccg ccggacgcaa gtaagtagtt ccccttcaac ccgagttaca ccgttcattt
     1561 gacagacccc cgctggctcc tgccgacctg gtatgtcatg tga
//
LOCUS       DATADN010000457         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1248063, whole genome shotgun sequence.
ACCESSION   DATADN010000457 DATADN010000000
VERSION     DATADN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248063"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VHH53_05940"
     CDS             <1..1237
                     /locus_tag="VHH53_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX2289730.1"
                     /translation="GEGQVVAQTAPQCFDISLWQLVSALLVGGTTLLVEQEVILDAAR
                     FVDKIVDGRVAVLQVVPSYLEVVLSYLEQHPHELPDLRCVSVTGETLKKELTQRWFAA
                     EPGIKLINAYGLTETSDDTNHEVMARVPDRERVPLGPPVNNVHVYIVDEHLSPVPLGA
                     PGVIVFSGVCVGRGYVNDPERTRSAYLTDPHRNGQRLYRGGDYGRWQPGGKLEFLGRR
                     DTQVKIRGFRIEIGEIENTLVRVPGVRDGAVVVAEKADQSKHLLAFYSGQQPLDVHLL
                     RDTLGESLPEYMIPSTFHWRETLPLTANGKIDRKSLTALARELDGSDLTGLDYHGPST
                     PTEQRLAAAWAKVLGIPADQISRRDHFFDRGGTSLSAVNLVITLHRAVSLKDVNRHPI
                     LADLAALVEGRSQRRLAAS"
     gene            1276..>1624
                     /locus_tag="VHH53_05945"
     CDS             1276..>1624
                     /locus_tag="VHH53_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX2289731.1"
                     /translation="MSSSSLASLLDVELQPGKPPILRAEATSDAPGWAAEHRDALRTV
                     IAEHGSVLIRGIGLRDAAEVGAVFARLASGLMAEKEAFASRRTYSDGVYSSSTWPANQ
                     PMCMHHELSYTLEF"
BASE COUNT          271 a          536 c          541 g          276 t
ORIGIN      
        1 cggcgagggg caggtggtcg cccagaccgc gcctcagtgc tttgacatct cgctgtggca
       61 actggtgtcc gcgctgctgg tcggtgggac cactctgctg gtcgagcagg aggtgatcct
      121 ggacgccgcg cggttcgtcg acaagatcgt tgacggccgg gtcgccgtgc tccaggttgt
      181 gccgtcctac cttgaagtcg tgctgtccta tctggagcag cacccccacg agttgccgga
      241 cctgcggtgt gtgtcggtca ccggcgagac gctgaaaaag gagctcacgc agcgctggtt
      301 cgcggccgag cccgggatca agctcatcaa cgcgtacggg ctgaccgaga cctcggacga
      361 cacgaaccac gaggtcatgg ctcgggtgcc cgaccgggag cgagtcccgc tcggtccccc
      421 cgtcaacaac gtgcacgttt acatcgtcga cgagcacctg tcacctgtgc cgctgggtgc
      481 ccccggtgtg atcgtcttct ccggggtctg cgtgggccgc gggtatgtca acgaccccga
      541 gcgcacccgg tcggcctacc tgactgatcc gcaccgcaac ggccagcggc tctaccgggg
      601 cggcgactac ggccgctggc agcccggcgg caagctggag ttcctcggcc gccgggatac
      661 ccaggtcaag attcgtggct tccggatcga gatcggcgag atcgaaaaca ccctggtgcg
      721 ggtgcccggt gtccgcgacg gcgcggtggt ggtcgccgag aaggcggatc agagcaagca
      781 cctactggcc ttctactccg gccagcagcc actggacgtc cacctcctgc gggacaccct
      841 gggtgaatcg ctgcccgagt acatgatccc gtcgaccttc cactggcggg aaactctgcc
      901 gctgaccgcc aacggcaaga tcgatagaaa gtcactgacg gcgctggcca gagaactcga
      961 tggcagtgac ctcactgggc tggattacca cgggccgagt acgccgaccg agcagcggct
     1021 ggcggccgca tgggcgaagg tgctcggcat cccggccgac cagatcagcc ggcgggacca
     1081 cttcttcgac cggggcggca cgtcgctgtc ggcggtgaac ctggtaatca ccctgcaccg
     1141 tgcggtgtcc ctcaaagacg tcaaccggca cccaatcctc gccgatctgg ccgcgctggt
     1201 cgagggcagg tcccagcggc gcttggccgc ttcttgatgg gcttctttgt gactaaacac
     1261 tgaaaggaaa acgagatgtc atcctcatct ctggcgtcat tgctcgatgt ggagctgcaa
     1321 cccggcaaac ccccgatcct gcgggcggag gccaccagcg acgcgccggg ctgggcggcc
     1381 gagcaccggg atgcactgcg caccgtcatc gccgagcacg gttcggtcct gatccgcggc
     1441 atcgggctgc gcgacgcggc ggaggtcggt gcggtttttg cgcggctggc cagcggtctg
     1501 atggccgaga aggaggcctt cgcatcccgg cggacctact ccgacggcgt gtactcctca
     1561 tcgacgtggc cggctaatca gccgatgtgc atgcaccacg aactgagcta cacgctggag
     1621 tttc
//
LOCUS       DATADN010000458         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_797360, whole genome shotgun sequence.
ACCESSION   DATADN010000458 DATADN010000000
VERSION     DATADN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797360"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VHH53_05950"
     CDS             complement(<1..190)
                     /locus_tag="VHH53_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2289732.1"
                     /translation="MLITGFPAGMLACNCYVLAPRTGADAIVVDPGQRAMGPLGRILD
                     EHHLTPSAVLLTHGHIDHI"
     gene            308..1183
                     /locus_tag="VHH53_05955"
     CDS             308..1183
                     /locus_tag="VHH53_05955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2289733.1"
                     /translation="MPTNEQRRATAKRKLERQLERRAEKARKRRIYTIVGSAAAAIVV
                     IAAVVATVVVTNRDSGSNTASAATTTSTTAADQPAAPGQLPPFVAPAGLGEDCQYPPA
                     AEASKKVNPPRTGKVPTDPAEVSASMTTNQGNLGLILDNAKAPCTVNSFASLAQQGFF
                     DNTPCHRLTTSPTLSVLQCGDPTGAGTGGPGYQFDNEYPTNQYQPDDTKLLEPVLYPR
                     GTLAMANGGPNTNGSQFFLVYKDSQLPPGYTVFGKIDETGLATLDKIAAAGVAGGGED
                     GKPAQDVTIKSILLD"
     gene            1329..>2047
                     /locus_tag="VHH53_05960"
     CDS             1329..>2047
                     /locus_tag="VHH53_05960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2289734.1"
                     /translation="MFAPLGILFGHMSLSQIKKTGEEGRGLAIAGLVIGYLVTVLTVL
                     VIVLSVLFFAYVARNLEDMDLYPDYPPYTAEPVPFPESAELPPFDPPATLGSNCQYPA
                     TTEPASKPVKPPRVGRVPTVPEYVDAGIATNVGSIGLELDNAKAPCTVNNFASLAAQG
                     FFNKTTCHRLTTGPQLKVLQCGDPSGTGMGGPGYRFPNEYPTNQFRLTDPALKTPVRY
                     PRGTLAMANSGPGTNGSQFFLV"
BASE COUNT          381 a          718 c          650 g          298 t
ORIGIN      
        1 agatgtggtc gatgtggccg tgggtcaaca gcaccgccga cggtgtcagg tgatgttcgt
       61 ccaggatccg gcccaatggg cccatcgccc gctgaccggg gtcgacgacg atggcgtccg
      121 cgccggtccg cggcgccagc acgtagcaat tgcacgccaa catgcctgcc gggaacccag
      181 tgatcaacac ggcgtccagt ttcccacctc atcttgagac gggtggcggc gccactctca
      241 gcaacggctg gcacactcgt tgccgaccga cacagcactc gacacagcac taaggaggaa
      301 cgcggcggtg ccgaccaatg aacaacgacg tgcgacagcc aaacgcaaac tcgaacggca
      361 gctcgaacgt cgggcggaga aggcgcgcaa gcgccggatc tacacgatcg tgggctccgc
      421 cgcggccgcc atcgtcgtga tcgccgccgt ggtagcgacc gtggtcgtca ccaaccggga
      481 ctcgggctcc aacaccgcct cggcggccac caccacgtcg accacggccg cggatcaacc
      541 cgcagcgccc ggccagttgc ccccgttcgt cgcacccgcc gggctcggtg aggactgcca
      601 gtatccgccg gcggcggagg cgagcaagaa ggtgaacccg ccgcgcaccg gcaaggtgcc
      661 gaccgatcca gccgaggtca gcgccagcat gacgaccaac cagggcaacc tcggcctgat
      721 actcgacaac gccaaggccc cgtgcaccgt caacagcttc gccagcctgg ctcagcaggg
      781 cttcttcgac aacaccccct gccaccggct gaccacgagc ccgacgctgt cggtgctgca
      841 gtgcggtgac ccgaccggtg cgggcaccgg cgggccgggc taccagttcg acaacgagta
      901 tccgaccaat cagtaccagc ccgacgacac caagctgttg gagccggtgc tgtatccgcg
      961 cggcaccctg gcgatggcca acggcgggcc gaacaccaac ggcagccagt tcttcctcgt
     1021 gtacaaggac tcgcagttgc ctccgggcta caccgtgttc ggcaagattg acgagaccgg
     1081 cctggccacg ctcgacaaga tcgccgcggc gggcgtggcc ggcggcggcg aggacggtaa
     1141 gccggcgcag gacgtcacga tcaagtcgat cctgctggac tgatgacgtg accacaccac
     1201 cgccgtacgg cgcatatcca ccgccgccgt acggcgggta tccggcggac cacttcggat
     1261 acccggccgg atatccaccg ccgaagtcga ccaactcgct ggcgatcgtc tcgctggtgt
     1321 gtgccttcgt gttcgcgccg ctgggcatcc tgttcggcca catgtcgctg tcgcagatca
     1381 agaagacggg cgaagagggc cgcgggctgg cgatcgccgg gttggtcatc ggttacctgg
     1441 tcaccgtgct gacggtgctg gtcatcgtgt tgagcgtgct gttcttcgcg tacgtggcgc
     1501 gcaacctcga ggacatggat ctgtacccgg actatccccc gtacacggcc gagccggtgc
     1561 ccttcccgga gtcggcagaa cttccgccgt tcgacccgcc cgccacgctg gggtcgaact
     1621 gccaatatcc ggcgacgacg gaaccggcga gcaagcccgt caaacccccg cgggtagggc
     1681 gggtgccgac ggtgcccgag tacgtcgacg ccggcatcgc cacaaatgtc ggcagcatcg
     1741 gtctagagct ggacaacgcc aaggcgccct gcaccgtcaa caacttcgcc agcctggctg
     1801 cacagggctt cttcaacaag acgacatgtc accgcctcac gaccggcccc cagctgaagg
     1861 tgttgcagtg cggcgatccg agcggcaccg ggatgggcgg accgggctat cggttcccga
     1921 acgaatatcc gaccaaccag ttccgcctca ccgatcccgc gttgaagacg ccggtgcggt
     1981 atccgcgcgg aacgctggcg atggcgaact caggcccggg gaccaacggc agccagttct
     2041 ttctggt
//
LOCUS       DATADN010000459         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_510823, whole genome shotgun sequence.
ACCESSION   DATADN010000459 DATADN010000000
VERSION     DATADN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510823"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VHH53_05965"
     CDS             <1..956
                     /locus_tag="VHH53_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX2289735.1"
                     /translation="PPGSQVVVEMVVDFLDHAAYEHLASWRRQHEATGGTVVVDEVGP
                     ARLVDNGGHGPARRVWVPALPRAFSPWWVWQAQAQHADHGHGGLQPLLAGVREYHRRL
                     APELLPHLERLVDGQRPRALFLTCADSQVVPNIITSSGPGDLFTIRNLGNLVPPPGHG
                     SESSVAAALRYAVKHLQVPTILVCGHSGCGAMQALLDGGNIDGPLDQWLRWGLPSLTA
                     LRDGHPVGAAAAAEGRHEADQLSMVNVAHQIELLRTHPVVQDAVAAGRIQIAGLFLDI
                     PTARLLLLDPTTGRFLPVPDEPPEGLVRLRQDDGIAQKPPA"
     gene            complement(1076..1279)
                     /locus_tag="VHH53_05970"
                     /pseudo
     CDS             complement(1076..1279)
                     /locus_tag="VHH53_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          296 a          520 c          510 g          264 t
ORIGIN      
        1 tgccgcccgg cagccaggtg gtggtggaga tggtggtcga cttcctagac cacgccgctt
       61 acgaacacct cgccagctgg cgacgtcaac acgaggcgac cggcggcacc gtcgtcgtgg
      121 atgaggtggg gccggcgcgg ctggtcgaca acggcggcca cggacccgcc cgtcgagtat
      181 gggtgcccgc cttaccacgt gccttctcac cctggtgggt gtggcaggcc caggctcagc
      241 acgcagatca cggtcacggc gggttacagc cgctgctggc cggcgtgcgg gagtaccacc
      301 gccgcctggc gcccgagttg ctgccgcact tggagcggct ggtggatggg cagcgtcccc
      361 gagcgctgtt tttgacctgc gccgactccc aggtagtgcc caacatcatc accagcagcg
      421 gaccaggcga cctgttcacc atccgcaacc ttggcaatct agtgccgcca cctggccacg
      481 gatccgaatc ctcagtggcc gcggcgctgc ggtacgcggt gaaacacctg caggtgccca
      541 caatcctggt gtgcggacat tccggctgtg gggccatgca ggccctgctc gacggcggca
      601 acatcgacgg accgctggac cagtggctgc gctggggcct gccgagcctg accgccctgc
      661 gcgacgggca cccagtcgga gcagccgcgg cagccgaggg ccgacacgag gccgaccagc
      721 tatccatggt caacgtggcc caccaaatcg agctgttgcg cacccacccc gtcgtgcagg
      781 atgcggttgc ggccggtcga atccaaatcg ccggactatt cctagacatc cccacggcac
      841 ggctgcttct tctcgatccg accacggggc gcttcctgcc ggtgcccgac gagccacccg
      901 agggcctcgt cagattacgc caggacgacg ggatagcaca aaagcccccc gcttgaacaa
      961 gcacacccta tgatcacttg gatacttctc gaccacttaa tgacgcccga acaacaacac
     1021 caaacgaccg caaacatcga gcgtgcgtga tcacgaacaa cagaggtcat cacgtctaaa
     1081 ggcaacaacc gaaacagcag tccaagaatc cttggttcgg ggttgccgtt ttcctcgacc
     1141 gaagatcgtc gagggcgcgg atgaagaacc ggcgggtggc cgcgaggtcc cgcttctgag
     1201 acaccaggac gtcaatgacc tggtcgaact ggtcgattgc ctgatacaga tatattcatc
     1261 gtgcggcggc cttcacatag acacccccat gaccagcgat ccctggagca tgccggcaat
     1321 gcgggtgagt tgcggtggtt gtgtctttca gatctcgtgt gtgcttgctg gtcaatttgg
     1381 ggcttgtggt cgcggcctgg gtgcgtgcgg tagtcatccg catcggcatc aggacgggcc
     1441 ccgtcctaca ccggcggcag ccaggctgcc gggtaagtag tcgtctggga ccggtacgaa
     1501 gtgggcggct gcggggtcaa gtagcagcag ccgggcagtg cggatgtcca ggaacagacc
     1561 agccagctgc acccggccgg ctgcgacggc
//
LOCUS       DATADN010000460         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1117533, whole genome shotgun sequence.
ACCESSION   DATADN010000460 DATADN010000000
VERSION     DATADN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117533"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VHH53_05975"
     CDS             complement(<1..831)
                     /locus_tag="VHH53_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2289736.1"
                     /translation="MSSERLIEWTGERCVPWADDLQVIYEHYHRYALASRFTTGARVL
                     DLGCGEGFGPALLAAEARKVVGIDIDPQTVEHAATHHSRGNLEFSLGSMIDPELLAGA
                     PPFDVITCFEALEHVEEQDTLVAVVRRLLAPGGLFLTSTPDVAVYSHQHGNDNPFHVR
                     ELTDAQFRTLLGDSFNHVAVLRQNVAVGSLITGQAQQGPAVAHSLRQAPDGGWRVTAD
                     LPHTYLLGVASDAALPQLPQLSALVDADLTLVRNAQRALAQEQGRTFELRTELAEEQQ
                     R"
     gene            902..2170
                     /locus_tag="VHH53_05980"
     CDS             902..2170
                     /locus_tag="VHH53_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289737.1"
                     /translation="MSVTGRRHIDVDQAPRQHPERRPCRGGRRFTALLCAVMFFLSPT
                     IALSLGAQAARFENRPLAAFGDPRDGWPWLAGLPAWAADNLPFRDGAVRMAEAVSRNL
                     FSEPARLPVPAVQSPVGPRAMAPIMPAAHAFPSVIEGRDGWLYLGADVSGACLPSRSL
                     DDTLDSLRRIRGVVESSGRQFVLVVAPDKTTMVPEYLPAEYVGRDCSAQARGEFWRRV
                     VAEAGAIDLRPALAEIARRKASPVYYPADTHWSQEGGLAMTYAVADRIASGITQSWQI
                     APAGSIPWPDDISPLIGRSGERAIPTYSLAPAGLPDRTRPVISDFRVPLHLSSAPAAG
                     TVDRPVRMIADSFTQFASRYLAATFTNITVVHPDTAAAAPWSAGELLAGGDVVVLEVA
                     ERNLLSGKSPILLHPVITEIGSQLASRPVR"
BASE COUNT          384 a          863 c          840 g          439 t
ORIGIN      
        1 tcgttgctgt tcctcggcga gctcagtgcg cagttcgaag gtccgaccct gttcttgggc
       61 cagcgcgcgc tgtgcgttgc gcaccagggt cagatcggca tcgaccagcg cgctgagctg
      121 gggcagctgc ggcagcgccg catccgaggc gacgccgagc aggtaggtgt gcggcaagtc
      181 cgctgtcacc cgccagccgc cgtccggtgc ctgccgcagc gagtgcgcca ctgccggccc
      241 ttgctgtgcc tgcccggtga tcagcgatcc gactgcgacg ttctggcgca ggacggcgac
      301 gtggttaaac gagtcaccca gcagggtccg gaactgcgca tccgtgagct cccggacatg
      361 gaaagggttg tcgttgccgt gctggtggct gtacacggcc acgtcaggcg tactcgtgag
      421 gaacagccca cccggtgcca gcagccggcg caccactgct accagagtgt cctgctcctc
      481 tacgtgttcg agggcctcga agcaggtgat gacgtcaaac ggtggtgccc cggcgagcag
      541 ctcgggatcg atcatcgacc cgagcgagaa ctccaggttg cccctcgagt ggtgagtagc
      601 ggcgtgctcc acggtctgcg gatcgatgtc gatgcccacc accttgcggg cctcggcggc
      661 gagcagcgcg ggaccgaagc cctccccgca acccaggtcg agcacccgtg ctccggtggt
      721 gaagcgggaa gccaacgcgt accggtgata gtgctcgtag atgacttgca ggtcgtctgc
      781 ccacggcacg caacgctcgc cggtccactc gatcagacgt tccgaagaca ttcgcgttct
      841 cccacgttcg gcgggtcggc cggtgcaggc taccgatcgg gtcgcggtta cggtgcacgc
      901 catgtcggtg accggccgca ggcacatcga cgtcgaccaa gctccccgac agcatccgga
      961 gcgccgcccg tgtcgtggcg gtcgtcggtt caccgcgttg ttgtgcgcgg tcatgttctt
     1021 cctcagtccg actatagcgt taagcctcgg cgcccaggcg gcccgattcg aaaaccggcc
     1081 gctggccgcg ttcggtgacc cgcgagacgg ctggccgtgg ctggccgggt tgcctgcttg
     1141 ggccgccgac aacctgccgt tccgagacgg cgcggtccgg atggcagagg cagtgagcag
     1201 gaacctcttc agcgagccgg cacgccttcc ggtgccggcg gtgcagtcac ctgtgggacc
     1261 ccgggcgatg gccccgatca tgcctgccgc ccacgcgttt ccttcggtga tcgagggccg
     1321 cgacggctgg ctttacctcg gtgcggatgt cagcggtgcg tgcctgccgt ctcgctcgct
     1381 ggatgacacc ctggattcct tgcgccggat ccgtggtgtg gtggagtcct ctgggcggca
     1441 gttcgtcctg gtggtggctc cggacaagac gaccatggtg cccgagtacc tgcccgcgga
     1501 atacgtggga cgggactgct cggcacaggc caggggtgag ttctggcgtc gggtggtcgc
     1561 cgaagccggc gcgatcgacc tgcggccggc gctggccgag atagcccggc gcaaggcttc
     1621 tccggtctac tacccggccg acacccactg gagccaggaa gggggcctgg cgatgaccta
     1681 cgcggtggct gaccgcatcg cctccggtat cacccaaagc tggcagatcg ccccggccgg
     1741 gtcgatcccg tggcccgatg acatcagccc gctgatcgga cgtagcggtg agcgagcgat
     1801 cccgacctac tcgttggcgc cggccgggtt gccggaccgc actcgcccgg tcatcagtga
     1861 cttccgggta ccgctgcatc tgtcctcggc gcccgctgcg ggcaccgtcg accggcccgt
     1921 tcggatgatt gccgactcgt tcacccagtt cgccagccgc tatctggcgg cgaccttcac
     1981 caacatcacc gtcgtgcacc ccgataccgc cgcagctgct ccatggtcgg ccggggaact
     2041 gctcgccggc ggcgacgtcg tcgtcctgga ggtcgccgag cgcaaccttc tcagcgggaa
     2101 atcaccgatc ctgctgcacc cagtgatcac cgagatcggc tcccagctag ccagtcgccc
     2161 ggtgcgctga catccgagag tcagcccagc gccttgacac agccgttgag ccacgccgag
     2221 gcgctgactt gtgggccagt tcgtcgcatg gcttcatccg cgcatcttgt ggcacccgga
     2281 tggtgctgct gcgaccactg gaatccatcc cggtaagacc tgtctcgcaa cgcgtcagcg
     2341 tcgtacccga cagaggccag ccagcacggc accgttgtgg ctatcacgac cgcgacaatg
     2401 ccgacgtcaa cccgattcat ggccttgcaa tgctcctagg gcatccggta gctgctgtgg
     2461 ggcagcttag ccgggtagcc ggcgacggcc agcgatgccc aggtcagacc tgtggtacca
     2521 ctccaa
//
LOCUS       DATADN010000461         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_364858, whole genome shotgun sequence.
ACCESSION   DATADN010000461 DATADN010000000
VERSION     DATADN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364858"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /gene="cydD"
                     /locus_tag="VHH53_05985"
     CDS             complement(<1..1056)
                     /gene="cydD"
                     /locus_tag="VHH53_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEX2289738.1"
                     /translation="MKPIDPRLLRYARATRVFLVAVIALGAIGAGLVIAQAMLIAEVV
                     VGAFQHRMAVSELGTPLLLLVAAAVGRALVGWFTELAAHRASAAVKSELRGRLLERAT
                     ALGPGWLSGQRTGSLVALATRGVDALDDYFSRYLPQLGLAVVVPVAVLARIVTEDWVS
                     AAIIVGTLPLIPVFMVLIGWATQSRMDRQWRLLSRLSGHFLDVVAGLPTLKVFGRAKA
                     QAESIRRITGEYRRATMRTLRIAFISSFALELLATISVALVAVTIGMRLVHGEMDLYI
                     GLVILILAPEAYLPLRQVGAQYHAAAEGLAAAEEIFSVLETPVPDSVTAAVPAGGLSF
                     EGVTVRYPGRSGDAVADV"
     gene            complement(1069..>1544)
                     /locus_tag="VHH53_05990"
     CDS             complement(1069..>1544)
                     /locus_tag="VHH53_05990"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX2289739.1"
                     /translation="VFVGLKTVGEIRERARKLALRVGLVTALLALAFLLWTQSDRGDG
                     ASLVALVAAVASLVLALVAARAKREGWAFALSGLTIVATVAMLFLTLFPNVMPSTLNP
                     DWSLTVTNASSSPYTLKIMTWCAAIATPVVMVYQGWTYWVFRKRIGTQHIAETVH"
BASE COUNT          276 a          579 c          510 g          179 t
ORIGIN      
        1 cacgtctgcc acggcgtccc cggacctccc gggatagcgg actgtgactc cctcgaagga
       61 caggccccct gccggcacgg cagcggtgac cgagtccggc actggtgtct ccagcaccga
      121 gaagatctcc tcggcggcag cgagcccctc cgccgccgcg tggtactgcg ccccgacctg
      181 ccgcaacggc agatacgcct ccggcgccag gatcaggatc acgaggccga tgtacaggtc
      241 catctcgccg tgcaccagcc gcatgccgat cgtcacggcg accagcgcca ccgagatcgt
      301 cgcgagcagc tccagcgcga aggaggagat gaaggcgatc cgcagagtcc gcatggtcgc
      361 ccgccggtac tcgccggtga tccgccggat cgactcggcc tgagccttgg cccgcccgaa
      421 gaccttcagt gtcggcagcc ccgcgaccac gtccaggaag tgaccggaca gccgggacag
      481 cagccgccac tggcggtcca tccgggactg ggtggcccag ccgatcagca ccatgaagac
      541 cgggatgagc ggcagggtgc ccacgatgat cgccgcggag acccagtcct cggtgacgat
      601 ccgcgccagc accgccaccg gcacgaccac cgcgagaccc aactgcggca gataccgcga
      661 gaagtagtcg tcgagggcat cgacaccccg ggtggccaga gcgaccagcg aaccggtccg
      721 ctgcccgctc agccatcccg ggccgagggc cgtcgcccgc tccagcagcc gcccccgcag
      781 ctcggacttc accgccgcgc tcgcccggtg cgcggcgagc tccgtgaacc agccgaccag
      841 cgcccggccg accgcggcgg cgaccaggag cagcagggga gtgccgagtt cggacacggc
      901 catgcggtgc tggaacgcgc cgaccaccac ctcggcgatg agcatcgcct gggcgatgac
      961 cagtcccgcc ccgatcgccc ccagggcgat gaccgccacc aggaagaccc gggtggcacg
     1021 ggcgtaccgg agcagacgcg gatcgattgg tttcacgtga aacacacctc agtgcacggt
     1081 ctcggcgatg tgctgcgtac cgatgcgctt gcggaacacc cagtacgtcc agccctggta
     1141 gaccatcacg accggagtgg cgatcgccgc gcaccaggtc atgatcttca gcgtgtacgg
     1201 gctggacgag gcgttggtga ccgtcaggct ccagtccggg ttcagcgtcg acggcatgac
     1261 gttcgggaac agcgtcagga agagcatggc caccgtggcc acgatggtga gccccgacag
     1321 ggcgaacgcc cagccctcgc gcttcgcccg cgccgctacc agcgccagga cgagcgacgc
     1381 cacggccgcg accagcgcga cgagcgacgc cccgtcgcct cggtcggact gcgtccacag
     1441 caggaaggcg agggccagca gggcggtcac cagcccgacg cgcagcgcca gcttccgcgc
     1501 ccgctcccgg atctccccga cggtcttgag cccgacgaac accg
//
LOCUS       DATADN010000462         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_967436, whole genome shotgun sequence.
ACCESSION   DATADN010000462 DATADN010000000
VERSION     DATADN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967436"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..441)
                     /locus_tag="VHH53_05995"
     CDS             complement(103..441)
                     /locus_tag="VHH53_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289740.1"
                     /translation="MIELRINAEDPQRFLPGPGAVTEWVEPAGDGVRVDAGYAKGITV
                     TPLYDSLLAKLVVHGRDRDQALQRARAAVAGFTVAGPKCNLPFHAELLEHPEFVSGRY
                     DTGLVARMRS"
     gene            699..>1611
                     /locus_tag="VHH53_06000"
     CDS             699..>1611
                     /locus_tag="VHH53_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289741.1"
                     /translation="MGPSAEAISLMGDKVTARDTVAVAGVPVVPGSHGAVTQPDAALA
                     QAQRVGFPVMLKAAAGGGGMGMAVAADADTLCAEHARVSAFAQRLFGDPRLFVERYFP
                     RVRHVEVQVLGLADGRVLALGERDCSVQRRHQKVAEESPSPAVEPDLRRRMEHAAVLA
                     ASSVDYRNAGTVEFLLDVETGEFFFLEMNTRLQVEHPVTELRYGVDLVAAQLCIAEGA
                     EPDFDVDTPVPNGHVIELRINAEDPQRFLPGPGAVTEWVEPTGEGVRVDAGYAKGTTV
                     TPLYDSLLAKLVVHGRDRDQALQRARAA"
BASE COUNT          250 a          518 c          585 g          258 t
ORIGIN      
        1 tcaaaagcga ggtgcgagcc tggaccggtg gcgctgttca tcacctggcg tctcgacatg
       61 ccagcgcgac acaccgtgca ccagcgctgg acacgcgggg ggttaagagc gcatccgggc
      121 aacgagcccg gtgtcgtacc gcccagagac aaactcgggg tgctccagca gctcggcgtg
      181 gaagggcaag ttgcacttcg gcccggccac ggtgaacccg gccaccgccg cccgcgcccg
      241 ctgcagggcc tggtcgcggt cgcgaccgtg taccaccagc ttggccagca aggagtcata
      301 cagcggcgtc accgtgatgc ccttcgcata gccggcgtcc actcgcaccc catccccggc
      361 gggctccacc cactcggtga ccgccccggg acccggcaga aaccgctgag gatcctccgc
      421 gttgatccgc agttcgatca catgtccatc gggaacagca gtatcgaggt caaaaccggg
      481 ttcggcgccc tcggcgatgc gcagctgagc tgcccttcgt taccgaggcc gacgaggcgg
      541 tcgagctcaa cgggccaccc gcgcaggctt accgggactc ctgccagcta ctcgacgccg
      601 cacgccgcac cggcgcggtt gcagtacatc ctggctacgg gttcctgtcc gaggacgcgg
      661 gatttgctcg ggcggtccgc gatgccggcc tggcgtgggt gggtcccagc gctgaggcga
      721 tctccttgat gggtgacaag gtgaccgcac gcgacaccgt ggcggtcgcc ggagtgccgg
      781 tggtgcccgg ttcacatggc gcagtgacgc agccggacgc cgcgctggcg caggcgcagc
      841 gggtgggttt cccggtgatg ctcaaggccg ccgccggggg aggcgggatg ggcatggcgg
      901 tggcggctga cgccgacacg ctgtgcgccg agcacgccag ggtgagcgct tttgcgcaac
      961 ggctcttcgg tgacccgcgg ctgttcgtcg agcggtactt cccgcgggtg cgccacgtcg
     1021 aggtgcaggt tctcgggttg gccgacggca gggtgctggc gctgggagag cgcgactgct
     1081 cggtgcagcg gcgacaccag aaggtggccg aagagagccc gtcacctgct gtcgagccgg
     1141 acttgcgccg ccggatggaa catgccgcgg tgctggctgc gtccagtgtg gattaccgga
     1201 atgcgggaac ggtggaattc ctgctcgatg tggaaaccgg cgagttcttc ttcctggaga
     1261 tgaacacccg gctgcaagtg gagcatcccg tcaccgagtt acgttatggc gtggatctgg
     1321 tggcagctca gctgtgcatc gccgagggcg ccgaacccga ttttgatgtc gatactcctg
     1381 ttcccaatgg acatgtgatc gagctgcgga tcaacgccga ggatcctcag cggttcctgc
     1441 cggggcccgg ggcggtcacc gagtgggtgg agcccaccgg ggagggggtg cgggtggacg
     1501 ccggctatgc gaagggcacc acggtgacgc cgctgtatga ctccttgctg gccaagctgg
     1561 tggtgcacgg tcgcgaccgc gaccaggccc tgcagcgggc ccgggcggcg g
//
LOCUS       DATADN010000463         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1117656, whole genome shotgun sequence.
ACCESSION   DATADN010000463 DATADN010000000
VERSION     DATADN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117656"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VHH53_06005"
     CDS             complement(<1..276)
                     /locus_tag="VHH53_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX2289742.1"
                     /translation="MGRTLAAKVWDTHVVRRGEGNGDSAAPDLLYIDLHLVHEVTSPQ
                     AFDGLRLAGRPVRRPDLTIATEDHNVPTMDIELPIADEVSRTQVDTLR"
     gene            344..1045
                     /locus_tag="VHH53_06010"
     CDS             344..1045
                     /locus_tag="VHH53_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2289743.1"
                     /translation="MGQHSGIGVLDKAMTVLRAIAANPCGLAELCSRTGLPRATAHRL
                     AVGLEVHRLLRRGSDGRWHPGPALHELANGALDPLLDAAAMVLPRLRDLTGESVQLYR
                     RDGMVRVCVAAAEPHSGLRDTVPVGARLPMTAGSGAKVLAAWADPATQRAVLGEAVFG
                     ERILMEVRRRGWAQSVAERESGVASVSAPVRDAAGSVVAAVSVSGPVERIGRRPGVRW
                     AADLLAAAEALHNRL"
     gene            1340..1555
                     /locus_tag="VHH53_06015"
     CDS             1340..1555
                     /locus_tag="VHH53_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2289744.1"
                     /translation="MDAKPKRSTSLRRLANIAANPRVAVVVDHYEDDWHALRWVRSDG
                     TGRLLAAEEPECRYAIMRLVARYPAVP"
BASE COUNT          273 a          584 c          610 g          290 t
ORIGIN      
        1 gcgcagcgtg tccacctggg tgcgggacac ctcgtcggcg atcggcaact cgatgtccat
       61 ggtgggcacg ttgtgatcct cggttgcgat cgttagatct ggccggcgga ccggccgacc
      121 cgccagccgc agcccatcga atgcctgcgg gctggtgact tcgtgcacca ggtgcaggtc
      181 gatgtagagc aggtcgggag ccgcgctgtc gccgttgccc tcaccgcggc gtaccacgtg
      241 ggtgtcccag actttcgctg cgagcgtgcg tcccatcgct ggttcctccg ctggacttct
      301 cacatgctgg acttcccata tcgcgggaag ttagtatcga tgtgtgggac agcatagcgg
      361 catcggggtg ctggacaagg cgatgacggt actgcgcgcg atagccgcga acccgtgcgg
      421 cctcgccgag ctatgttccc gcacgggttt accccgggcc accgcgcacc ggctggcggt
      481 aggactggaa gtgcaccggc tgttgcgtcg cggcagcgac ggccggtggc atcccggacc
      541 cgccctgcac gagctggcca acggtgctct cgacccattg ctcgacgccg cggcgatggt
      601 gctgccccgg ctgcgtgacc tcaccggaga aagcgtccag ctctaccggc gggacgggat
      661 ggtccgggta tgcgtggcag ctgccgaacc gcacagcggc ctgcgtgaca ccgtcccggt
      721 gggggcccgg ctgccgatga ccgccggatc gggagccaaa gtcttggccg cgtgggccga
      781 tccggccacc caacgtgccg tgctcggcga agccgtgttc ggcgagcgaa tcctgatgga
      841 ggtgcgccgg cggggctggg cgcagagcgt ggccgagcgc gagtccggag tggccagcgt
      901 gtccgccccg gtgcgcgatg cggccggcag cgtggtggcc gcggtgtcgg tgtccggccc
      961 cgtggagcgg atcggccggc ggccgggcgt ccgctgggcc gccgatctgc tggccgccgc
     1021 cgaggccctg cacaaccggc tgtgaataca cctatgcagt aggcaatcac ttactcggtg
     1081 gacagcaggc ttaacagtcc agctggcaca ttgctgcgcg cccctggatc agtgggagaa
     1141 cgcgaccagc cggtcgtaga cggatgactg acgccgacgc acgccgacgc ttcgcagagg
     1201 cccgagtcac ccggctggca actgcggtcg tcaacggcca cccacacgtc gttccgatga
     1261 cctttgcgct ggcgggcgga acggataccc gtgtggcagg tgagggcaaa gctatcccgg
     1321 tgacacggtt tacagcgccg tggacgcgaa gcccaagcgt tctacgtcgc tgcgcaggct
     1381 cgccaacatc gcggccaatc cgcgcgtcgc cgtcgtcgtg gaccactacg aggatgactg
     1441 gcacgcgctg aggtgggtgc gatccgacgg tacgggccgg ctcctcgccg ccgaggagcc
     1501 cgagtgccgc tacgccatca tgcggctggt cgctcgctac cccgcagtac cgtgagcagc
     1561 caccacgtgg tccgatggta gctatcgacg tacacactgg tcgagctggt ttggccagcg
     1621 gcgactgacc tctcaggctt ctccttagct ctctacaagc caaaatatgt agccccgacg
     1681 ggatttgaac ccgcgctacc gccttgagag ggcggcgcga tcggcacctc ctcggtgtca
     1741 ggacctgcac aaacgac
//
LOCUS       DATADN010000464         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1117690, whole genome shotgun sequence.
ACCESSION   DATADN010000464 DATADN010000000
VERSION     DATADN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117690"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1469
                     /locus_tag="VHH53_06020"
     CDS             51..1469
                     /locus_tag="VHH53_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HEX2289745.1"
                     /translation="MARVGLLTFSDGRDFVHADIKSFGQDVEGRIAQALEQAGHEVIR
                     ATEPIWTNTLATAEARRVADARPDLTIFNYPVWAFPHFSMLAATASPGPLLLFSNIDP
                     QFPGMVGMLAAGGGLDQIGRQHTRVWGDMTDPAVRDRVLETVRAAAAVSTLSGSTFGR
                     IGGRPMGMYTAVADTDQWMSTFGVDVEEIDQWEIVRRSDSVEQSRVTAGREWLERHAA
                     GVHYDGKQLTQELLERQIRSYHAVRELIDEWNLDFCGIKGQPELTTHFTTMDVTEAFL
                     NDPYDWEGSKQPHVCATESDMDGALTMQLLKGLTGTPVLFADVRHYHADRDIWDLCNS
                     GQHATWYAARSDDPAENLRAVHFYPEIFYFPAGGASVSFFAAPGQATFARLTRIAGRY
                     RMHVARGAFESYDDATNEALARQSTFEWPHAFARLEVEAGEFLSSFGANHIHAVPGEH
                     VAELRAVCRMLDIEWAGLGTAQ"
     gene            1477..>2142
                     /locus_tag="VHH53_06025"
     CDS             1477..>2142
                     /locus_tag="VHH53_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX2289746.1"
                     /translation="MSSLLLGVDIGTASSKGVLVHADGTVVATATRPHEVSMPHPGWV
                     EHDPQAVWWADFVGISKELAAVAPEPVAAVGISGIGPCLLPVDSEDRPLRPAILYGVD
                     TRATVEIDELTQTLGKQTILDRCGSVLTSQAVGPKLAWLRANEPETWAATRRFFMAST
                     FIVRKLTGSYILDHHSASQCTPMYDPVEHCWIDEWTELIAPGLELPQLRCPAPRRPLR
                     RASA"
BASE COUNT          358 a          747 c          705 g          332 t
ORIGIN      
        1 tcgtcactca gcgaaacggg caccggtcgt cctagcaggc aggagcgggt atggcacgcg
       61 tcggactgtt gaccttctcc gacggacgcg acttcgtcca cgccgacatc aagtcgttcg
      121 ggcaagacgt cgaaggccgc atcgctcagg cgctcgaaca agccgggcac gaggtcatcc
      181 gcgcgaccga gccgatctgg accaacacgc tggccaccgc cgaggcccgc cgggtcgctg
      241 acgcccgacc agacctcacc atcttcaact atccggtgtg ggccttccca cacttctcga
      301 tgctcgccgc gacagcttcc ccgggtccgc tgctgttgtt ctccaacatc gacccgcagt
      361 tccccgggat ggtcggcatg ctggcggccg gcggagggct ggaccagatc ggccggcaac
      421 acacccgggt gtggggtgac atgaccgacc cggccgtgcg ggaccgcgtc ctggaaaccg
      481 ttcgcgccgc ggcggcggtg tccacgctgt ctggcagcac cttcggccgc atcggtggcc
      541 gtccgatggg catgtacacc gcagtggccg ataccgatca gtggatgagc acgttcgggg
      601 tggacgtcga ggagatcgac cagtgggaga tcgtgcggcg ctcggacagc gtcgagcagt
      661 cgcgggtgac tgccggccgg gaatggctgg agcgccacgc cgccggggtg cactacgacg
      721 gcaagcagct gacgcaggaa ctgctcgaac gccagatccg ctcctaccac gccgtgcgtg
      781 aactgatcga cgaatggaac ctcgacttct gcggcatcaa gggccagccc gagctaacca
      841 cgcacttcac cacgatggac gtcaccgagg cattcctcaa cgatccctac gactgggagg
      901 gctccaagca gccacatgtc tgtgccaccg agtccgacat ggacggtgcg ctgaccatgc
      961 agctgctcaa gggcctgacc ggcacgccgg tgctgttcgc cgacgtgcgg cactaccacg
     1021 ccgaccgcga catctgggac ctgtgcaatt ccgggcagca cgccacctgg tacgccgcgc
     1081 gcagtgacga cccggcggag aacctgcgcg cagtgcactt ctaccccgag atcttctact
     1141 tccccgccgg tggtgcctcg gtgtcgttct tcgccgcgcc tgggcaggcc accttcgccc
     1201 ggctgacccg gatcgcgggc cggtaccgga tgcacgtcgc ccgtggcgcg ttcgagtcct
     1261 acgacgacgc aaccaacgag gcgctggctc ggcagtcgac gttcgagtgg ccacacgcgt
     1321 tcgctcggct ggaagtcgag gccggcgagt tcctgtccag cttcggagcg aaccatatcc
     1381 atgcggtgcc cggtgagcac gtggccgagc tgcgggcggt gtgccgcatg ctcgacatcg
     1441 agtgggccgg gctgggtacg gcccagtgag gacaccgtga gctcgctgct gctcggtgtc
     1501 gatatcggca ccgccagctc caaaggcgtg ctggtccacg ccgacggtac cgttgtcgcg
     1561 acggcgaccc ggcctcacga ggtgtcgatg ccacaccccg gctgggtgga gcacgacccg
     1621 caggcggtgt ggtgggccga tttcgtaggg atcagcaagg agctggcggc ggtggcgccc
     1681 gaaccggtcg ccgcggtcgg catcagtggc atcggtccgt gcctgctccc ggtggacagc
     1741 gaagaccggc ccctgcggcc ggcgatcctc tacggcgtgg acacccgcgc caccgtcgag
     1801 atcgatgagc tgacccagac gctgggcaag caaaccatcc tggaccgctg cgggtcggtg
     1861 ctcacctcgc aagcagtcgg acccaagctg gcgtggctgc gcgcgaacga acccgagact
     1921 tgggccgcaa cccgccgctt cttcatggcc agcaccttca tcgtgcgcaa gctcaccggc
     1981 agctacatcc tcgatcacca ctcggccagc cagtgcaccc cgatgtacga cccggtcgag
     2041 cactgctgga tcgacgaatg gaccgagctg attgcccccg gcctggagct gccgcagctg
     2101 cgctgccctg cacctcgccg accacttcgg agggccagcg ca
//
LOCUS       DATADN010000465         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_797932, whole genome shotgun sequence.
ACCESSION   DATADN010000465 DATADN010000000
VERSION     DATADN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797932"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VHH53_06030"
     CDS             <1..419
                     /locus_tag="VHH53_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEX2289747.1"
                     /translation="PQEPVSVPRPVPKRPRPFDEWGKAEASASAVRPYTWTGGRTSPV
                     FDLEVETLVSTSEHGRDVAALTSVEHRAVAELCRDPRSVAEVAALLSLPLGVARVLLG
                     DMADIGLIVVHGKANTSGDTPEIALMERVLSGLRRL"
     gene            532..1080
                     /locus_tag="VHH53_06035"
     CDS             532..1080
                     /locus_tag="VHH53_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX2289748.1"
                     /translation="MSAKIVVAGGFGVGKTTLVGSVSEIAPLTTEAVLTDASIAVDDL
                     AATPDKSTTTVAMDFGRVSLDAELILYLFGTPGQHRFWFMWDDLVRGAIGAVVLVDTR
                     RLADCFAAVDFFEDRGLPYLVGVNCFDGVLSHSVEDVREALAIDSAIPIIPCDARERE
                     STKKTLIALVEHAMRRSVGARN"
     gene            1323..>1735
                     /locus_tag="VHH53_06040"
     CDS             1323..>1735
                     /locus_tag="VHH53_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HEX2289749.1"
                     /translation="MTATLAGDSAAPPHRRSGGWRLQDWRLRTKLTAVLLVPLVLAGV
                     LGGLRVSDLMRQANDLAVVVRQVGFAQQVGLAVHDLQGERHLAAVALVTDRPADRVAL
                     QAQYQRVDAAVTILRTGDFGSNALAPTAAYQAALGR"
BASE COUNT          260 a          552 c          609 g          314 t
ORIGIN      
        1 ctccgcagga accggtttcg gtgccgcggc cggtcccaaa aagacctagg cctttcgacg
       61 agtggggcaa ggcggaggcc tcagcttccg cggtacggcc ttacacctgg actggtgggc
      121 gtacctcgcc ggtgttcgac ctcgaggttg agacgctggt atccaccagc gagcacggcc
      181 gcgatgtagc ggcgctgacc agtgtggaac atcgggccgt ggccgagctt tgccgtgacc
      241 cccgatcggt ggccgaggtc gccgctctac tatccctgcc gctgggcgtg gcccgggtcc
      301 tgctgggtga catggcagac atcggcttga tcgtggtgca cggcaaagcg aacacctctg
      361 gtgacacgcc ggagattgcc ttgatggaaa gggtgttgag tggacttcgt cgcctctgat
      421 cggaccagct ccccatcggt cccatcggcg cagtgggcac catcagtcca acccggttta
      481 ggtgcgcccg gtcagccccc cggggggcag cccacgggtg gggccggcgc gatctccgcg
      541 aaaatcgtcg tggccggggg cttcggcgtg ggcaagacca ctctggtcgg ctccgtttcc
      601 gagatcgcgc cgctgactac cgaagcagtg ctcactgatg cgagcatcgc ggtggatgac
      661 ctcgcggcca caccggacaa gtcgaccacc acagtggcaa tggacttcgg tcgcgtctca
      721 ctggatgcag agctgatcct gtacctgttc ggcactccag ggcagcaccg cttctggttc
      781 atgtgggatg acctcgtgcg tggcgcgatc ggtgcggttg tcctggtgga cacccgccgg
      841 ctggctgatt gctttgcggc ggtggacttc ttcgaggatc gcggcttgcc ttacctggtc
      901 ggggtgaatt gtttcgatgg cgtgctcagc cacagcgtcg aggacgtccg ggaagcgctg
      961 gccatcgatt ccgcgattcc catcatccct tgcgatgcgc gcgagcggga gtcgaccaag
     1021 aagacgctca tcgcgctggt cgagcacgcc atgcgtcgat cggtgggcgc tcgcaactga
     1081 cctgggattg ttcggagcga atccagcgca ggctgcgctg gttgacagac acgttaagtg
     1141 atgtacgggt cgcgagctgg gggcgacgtc gagttgggag agtgcgcagc gtggccgcca
     1201 cgaaggaagg gcaagacccc ctccgaccgc tccggcggtg tcatttgctg tgacgagatg
     1261 actgcgaacc tcggctgcgc ccctcccggt gccggcgtgc atgataccgc ggagccgcca
     1321 tcatgaccgc cacgctcgcg ggtgatagcg ccgcaccgcc gcaccgccgc agcggcgggt
     1381 ggcggctgca ggattggcgg ctgcgcacca aactcactgc ggtgctgctg gtaccgctgg
     1441 tgcttgccgg ggtgctcggc gggctgcggg tctccgacct gatgcgccag gccaacgacc
     1501 ttgcggtggt tgtgcggcag gtcgggttcg ctcagcaggt tggcctagcc gtgcacgatc
     1561 tgcaagggga acggcacctg gcggcggtgg cgctcgtcac cgaccgccct gcggaccggg
     1621 tcgcgctgca ggcgcaatac cagcgggtgg acgccgccgt caccatcttg cgtactgggg
     1681 acttcggttc caatgcgctc gcccccaccg cggcgtacca ggccgcgctc ggccg
//
LOCUS       DATADN010000466         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1117745, whole genome shotgun sequence.
ACCESSION   DATADN010000466 DATADN010000000
VERSION     DATADN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117745"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1148
                     /locus_tag="VHH53_06045"
     CDS             435..1148
                     /locus_tag="VHH53_06045"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX2289750.1"
                     /translation="MACPRLPATLRHLVDLSRETLPPMHPAGRPFVAGGAVATLLLRR
                     LSPTAATLGAVATACCAAFFREPHRVPPQDPILAVAPADGTVSSVALAVAPPELGFDD
                     QVRPRVSVFLSVFDVHVQRIPVDGEVRQVAYRPGMFLSADLDKASEANERASVLLRSA
                     TGHDLAVVQIAGLVARRIVCSVRPGDVVAAGSTYGLIRFGSRVDLYLPPGSAVLVRPG
                     QRTVGGETPLARLPVAGEF"
     gene            1152..2090
                     /gene="pssA"
                     /locus_tag="VHH53_06050"
     CDS             1152..2090
                     /gene="pssA"
                     /locus_tag="VHH53_06050"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX2289751.1"
                     /translation="MPGAGVRLLPNAITVLALCSGLSAVKFALDGNYAGALAAISLAA
                     LLDALDGRLARLLDATSRIGAELDSLADGISFGVAPAVVLFIWALDDTRAGWSVALVF
                     TVCVILRLARFNTLLDDTEALPFSKEFFVGVPSPSGALLALLPLTVFLQFGVGWWSSP
                     VTVAAWTVVVAVAMVSRLPTLSAKTVRVPAQAVAPLLVLIAGLAAAVITFPFASLGVA
                     TLLYVAHIPYASYRYRWLARHPSAWEVPPRQRRAIRRTARSMRRLGLRPPLRRRVAGA
                     AARAGAAVRRRRNGDRNGEVPRDGRRTRRIGLRRRP"
BASE COUNT          346 a          869 c          889 g          454 t
ORIGIN      
        1 cgcgctggcc cggtctgact gcctgatcga catcccggag tccacgacgc atctcgcggc
       61 aggagatgag gtagtcgtat tgcgcaccgg cggcacgtga gcagtcacat cttgcaggtg
      121 tgagaaaacc acaaccgtcg agcatggcct tgtgatcgag ggtgagccga agcacactaa
      181 gggtaagcaa ttgctcggcg agctgttgca tcccagaggc gcagtgttcc tgcgccgtcg
      241 gggggcgcag gggcgctggg aagaaaccgt tcgggaagct ggggagcggt cgaacggttt
      301 atgggcgccg gggatctgcc gtcgggggac ggtggatccc cggcgtctct cactacccgc
      361 cacccgcgta cggtgagccg caacctggcg tgcattgacc cgcccgtcat acgacccgag
      421 gacctcacaa acgcatggcc tgccctcgct tgcccgcgac attgcggcac ctggtggacc
      481 tatctcgcga gacgctccca ccgatgcacc ccgcaggtag accattcgtg gcaggaggtg
      541 ccgtcgccac gctgctgctg cgccggctgt ccccgaccgc cgcgaccctc ggcgccgtcg
      601 cgaccgcgtg ctgcgcagcg ttcttccgcg aaccccatcg ggtcccgccg caggatccga
      661 tactcgcagt ggcccctgcc gacggcacgg tgagcagcgt ggcactcgcc gtggcgcccc
      721 ctgagttggg gttcgacgat caggtacggc cacgggtcag cgtgtttctc tccgtgttcg
      781 acgtgcacgt gcaacgcatt ccggtggacg gtgaagtgcg ccaggtcgct tatcgtcccg
      841 ggatgttcct gtctgccgat cttgacaagg ccagcgaggc caatgagcgc gccagcgtgc
      901 tgctgcgctc ggccaccggg cacgaccttg ctgtggtgca gatcgccggg ctggtggcgc
      961 gccggatcgt gtgctcggtt cggcccggcg acgtggtggc ggccggatcc acctatgggc
     1021 tgatccgctt cggatcccgg gtggacctct acctgccacc gggcagtgcg gtgctggttc
     1081 gccccgggca gcgcaccgtg ggtggagaga ccccgttggc tcggcttccc gtggccgggg
     1141 agttctgaac cgtgcccggt gcaggggttc gcctgctacc caatgcgatc accgtgctgg
     1201 cgttgtgctc gggtctgtcg gcggtgaagt tcgccttgga cggcaactac gccggtgcac
     1261 tcgccgcgat ctcgttggct gcgctgctgg acgcactcga tggccggctg gcccggctac
     1321 tcgatgccac ctcccggatc ggcgccgaac tggactcact cgccgacggc atttctttcg
     1381 gcgtggcacc ggcggtggtg ctgttcatct gggcgctgga cgacacccgt gccgggtggt
     1441 cggtggctct ggtgttcacg gtctgcgtga tattgcggct ggcccggttc aacaccctgc
     1501 tcgacgacac tgaggcgctg ccgttctcca aggagttctt cgtcggtgtc ccgtctccat
     1561 ccggcgcgct gctggcgcta cttccactca ccgtcttcct ccagttcggc gtgggctggt
     1621 ggtcctcgcc ggtgaccgtg gcggcatgga cggtcgtggt ggccgtggcg atggtgagcc
     1681 ggttacccac cctgtctgcg aagacagtcc gggtaccggc gcaggcagtg gcgccgctgc
     1741 tggtactgat cgccggtctg gctgccgcgg tgatcacctt cccattcgcg tcgctgggcg
     1801 tggcgacgct gctgtacgtg gcccacatcc cctatgcgag ctaccgctac cgctggttgg
     1861 cgcggcatcc gtcggcgtgg gaggttccgc cccgtcagcg gcgggcgatc cgccgcaccg
     1921 cacgcagcat gcgccgcctc gggctacgcc cgccgctgcg acggcgggtc gccggtgccg
     1981 ccgcccgtgc tggtgccgcg gtgcgccgca gacgcaatgg agacagaaac ggcgaggtgc
     2041 cccgcgacgg ccgccgcacc cgccggatcg gcctgcgccg acgcccctag ccgcgcgtgt
     2101 ccggctctct tgcccggtgt gtcgcgctca ggtgcacgat gtgtgctcat cccaggtttt
     2161 cgacgggtag gcgcgcctca aggtgccgcg cgagggcctg acgaaggacg gcccggggtc
     2221 cgcgcgtcac ctcgtgacca accgagtgcg gggagcaagg ggaatcgggg cacgcgcgta
     2281 acgatgctcg tcagggctgc ttgacgccgg gcctccacca ttaccggtgc acgctcctac
     2341 cgtcgccgta ccaggagggc gatagccgaa cacgtacttg tcgtgcacca caggcgtaca
     2401 caccgggcac aagacgcgga cacgccggcg atggggaagc ggtactccca gtgcggtatc
     2461 ggctggtacg cccgctgatc gatgcccccg cacgggctgc tgactaccgt tgttcccgtg
     2521 gcacaagcag ccctgggcaa gccggccggt gccggcct
//
LOCUS       DATADN010000467         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_39823, whole genome shotgun sequence.
ACCESSION   DATADN010000467 DATADN010000000
VERSION     DATADN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39823"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..313)
                     /locus_tag="VHH53_06055"
     CDS             complement(59..313)
                     /locus_tag="VHH53_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289752.1"
                     /translation="MSPTVPAEVCVSRRDAVDRQVSIEMAWCPACRAECTVEIIALAG
                     DPAPVAICVDCGLGVETWWLPGLLTTTYDVEGAGHGVRAS"
     gene            422..1492
                     /gene="hemE"
                     /locus_tag="VHH53_06060"
     CDS             422..1492
                     /gene="hemE"
                     /locus_tag="VHH53_06060"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX2289753.1"
                     /translation="MTTTTGPATARRVLPDVPLLAAARGERPDHPPVWFMRQAGRSLP
                     EYRKVRAGLPMLQACMTPELACEITLQPVRRHGVDAAILFSDIVVPLHAAGVRLDIVP
                     GTGPVVDSPVRTASDVAALPALDPESVEPVAAAVRLLVNELGQIPLIGFAGAPFTLAS
                     YLVEGGPSRHHERTKALMHSEPEVWHALLDRLAELTLTFLRVQAAAGVDAVQLFDSWA
                     GALSQRDYRQFVLPHSARVLAGLAGTGLPRIHFGVGTAELLGAMAQAGADVVGVDWRI
                     PLDVAAQRVRASDAHGPVVLQGNLDPAVLLAGWPVVEREVHRVIAEGRAADAHVLNLG
                     HGVLPETDPDMLTRIVELAHSL"
     gene            1489..2976
                     /gene="hemG"
                     /locus_tag="VHH53_06065"
     CDS             1489..2976
                     /gene="hemG"
                     /locus_tag="VHH53_06065"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX2289754.1"
                     /translation="MNGAGPPRIAVVGAGISGLTAAYRLRTLLGPAAQIMLVEGSRRL
                     GGALRTVELAGLPLDVGAEAFLMRRPEAVRLIEELGLDEQLVHPTAVVPTVRAGGRTV
                     ALPTRTLLGLPGTAADVAGLLSAKGTQRVAAEPTVPLCWEPGQDAAVGMLLRERAGDE
                     LVDRLVDPLLGGVYAGRADALGLRATLPAVATALDRGAGSLLAAAAGTLPPLHGVSGS
                     HARRVGFSCTDPVFGTLLGGLGGLVDALAAAAAVQPRLGLLARGLARTSVGWRLELGG
                     RGLEAGRPERLDVDAVILAVPAPALRRLLADPLPLASAAAGRVEVASSAVVGLALPLA
                     AAQALPEASGVLVAASERLSVKAFTFSGRKWAHASSTEALVIRASLGRHGEARTLQCD
                     DAQLVDAVRADLAALTGITAPPVATVVARWGGGLPQYAPGHLDAVAALEREVAASAGL
                     AVAGATLHGVGVPACIATADTAARRIADYLASRQATAASGGTMGS"
     gene            2973..3221
                     /locus_tag="VHH53_06070"
                     /pseudo
     CDS             2973..3221
                     /locus_tag="VHH53_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
     gene            complement(3220..3345)
                     /locus_tag="VHH53_06075"
                     /pseudo
     CDS             complement(3220..3345)
                     /locus_tag="VHH53_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
BASE COUNT          428 a         1147 c         1234 g          536 t
ORIGIN      
        1 cgaaagcacc gccctgcgga agatctccgg ggtctccgcc agatcaggca caccgcggtc
       61 acgatgcgcg gacaccgtgg ccggccccct ctacgtcgta ggtcgtcgtg agcaacccgg
      121 gcaaccacca ggtctccact ccgaggccgc agtccacaca gatggcgacc ggcgcgggat
      181 cgccagccag tgcaatgatc tccacggtgc actcggcccg gcatgctgga caccaggcca
      241 tctcgatact gacttgccga tctacggcat cgcgccggga cacgcagacc tccgccggga
      301 cggtggggct cattccccga gcacacggtc gcacgagtgg gctgggcgat cagcgcgcca
      361 cgccggggcg gtccaccccg ggtctgcatc atgcccgatc cgtgcgacga tggtcgctga
      421 gatgaccacg accaccggac cggccaccgc ccgccgggtg cttcccgatg tgccattgct
      481 ggccgcagct cggggcgagc gcccggacca cccgccggtg tggttcatga ggcaggctgg
      541 gcggtcacta cccgagtacc gcaaggtgcg cgccggtctg ccgatgctgc aggcctgcat
      601 gactccagag ctggcgtgcg agatcacgtt gcagccggta cggcgccacg gggtggacgc
      661 cgccatcctg ttcagcgaca tcgtggtgcc gctgcacgca gctggcgtca ggctggacat
      721 cgtgcctggc accggtccgg tagtggacag tccggtgcgc actgcctcag atgtcgcggc
      781 gctgcccgcc ctggatcctg agtcagtcga gccggttgcc gccgcggtgc ggttgctggt
      841 caacgagctg gggcagatcc cgctgatcgg gtttgccggg gcaccgttca cgctggcctc
      901 ctacctcgtc gagggcggac cgagccgcca ccacgagcgc accaaggccc tcatgcattc
      961 cgagcctgaa gtctggcacg cgttgctgga tcggctcgcc gagctgaccc tcaccttcct
     1021 tcgggtgcag gcagccgctg gggtggacgc ggtgcagctg ttcgactcct gggcgggtgc
     1081 gctgtcgcag cgggactacc ggcagttcgt gctcccgcac tcggcgcggg tgctggcggg
     1141 cctggccggt accggcttgc cgcggattca ttttggggta ggtaccgcag agctgctcgg
     1201 cgcgatggct caagccggcg cagatgtggt cggagtggat tggcggattc ccctcgacgt
     1261 tgccgcgcaa cgagtgcggg cgtccgacgc gcacggcccg gtcgtgcttc agggcaatct
     1321 cgacccggca gtgctgctgg ccgggtggcc ggttgtcgag cgcgaggtgc atcgggtgat
     1381 cgcagagggg cgtgcggcgg acgcacacgt gctgaacctc ggtcacggtg tcctgccgga
     1441 aaccgatccg gacatgctga cccgcatcgt ggagctggcg cactcgctgt gaacggcgcg
     1501 gggcctccgc ggatcgccgt ggtcggtgcg ggaatctctg ggctgaccgc ggcgtaccgg
     1561 ttgcgcacgc tgctcggtcc ggccgcccag atcatgcttg tcgagggctc gcggcggctc
     1621 gggggagcgc tgcggaccgt ggagctcgcc ggcctgccac tggacgtggg cgcggaggcg
     1681 ttcctgatgc gccggccgga ggccgtcagg ctgatcgagg agctgggact cgacgagcaa
     1741 ctggttcatc ccaccgcggt ggtccccacg gtgcgcgctg gggggcgcac cgtggccctg
     1801 cccacccgca ccctgctggg actgccaggc accgctgctg acgtcgccgg cctgctgtct
     1861 gcaaaaggga cgcagcgggt ggccgcggaa cccacagtgc cgctgtgctg ggaacccggg
     1921 caggacgccg cagtggggat gctgctgcgg gagcgagccg gtgacgagct ggtcgaccgg
     1981 ctggtcgacc cgttgctggg cggagtgtat gcggggcgcg ccgacgcgtt ggggttacgt
     2041 gccacgttgc ccgcggtggc cactgcgctg gaccgcggcg cgggatcact gctagctgcg
     2101 gccgctggta cgttgccgcc cctgcacggc gtgtcgggct ctcatgcacg acgtgttggg
     2161 ttctcatgca cggatccggt cttcggcacc ctgctgggcg ggctcggtgg cctggtggac
     2221 gcgctggcgg ccgctgcggc ggtgcagccg cggctcgggc tgctggcgcg cggactggcc
     2281 cgtacctccg tcggctggcg gctggagctg ggcggtcggg gccttgaggc cggtcggccc
     2341 gagcgcctcg atgttgacgc tgtgatcctc gccgtgccag cccccgcctt acggcggctg
     2401 ctcgctgacc cactgccgct ggcctcggct gctgcaggcc gggtcgaggt cgcctcttct
     2461 gcggtggtcg ggctggcgct gccacttgcc gccgcgcagg ccctcccaga ggcgtccggc
     2521 gtgctggtgg ccgccagcga gcggctgtca gtcaaggcgt tcaccttttc cggccgcaag
     2581 tgggcgcacg cttcgtcgac cgaggcgctg gtgatccgag cgtcgctggg gcggcacggg
     2641 gaggcccgga cgctgcaatg tgacgacgca cagctggtcg atgcggttcg tgccgatctt
     2701 gcggcgctca ccgggatcac tgcccccccg gtcgccaccg tggtggcgcg ctggggcggg
     2761 gggctgccgc agtacgcgcc cggccacctc gacgccgtcg ccgcactcga acgggaagtc
     2821 gccgcgtctg ccggtttggc ggtggccggc gccaccctgc atggggttgg cgtgccggcg
     2881 tgcatcgcta ccgctgacac cgctgcccgt cgcatcgccg attacctggc cagccgccaa
     2941 gcaaccgcag cgagcggtgg gacgatgggg tcatgacccg ccttgactac gcaacgctga
     3001 actccaccat tcgctacacc atgtggtcgg tcttccgggt cgagcccggt cggttgcccg
     3061 ccgaccgcgc ggaatccgcg cgtgaggcgc agcactacct ggaggcgctg gagggcaagg
     3121 gagtggcggt gcggggagtc tatgacctgg ctggtctgcg cgccgacgcc gactggatga
     3181 tctggtggca cgccgaggag atcgaggcgc tccaggcggc caccagacca tatagtcggc
     3241 gtcggcccgc agcccggcga ggtcgtacac cccccgcacg gtgactccct tgccggccaa
     3301 ggcatccagg aactcctgag cctcgcgcgc gagcgggccg cgctc
//
LOCUS       DATADN010000468         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1249348, whole genome shotgun sequence.
ACCESSION   DATADN010000468 DATADN010000000
VERSION     DATADN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249348"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VHH53_06080"
     CDS             complement(<1..281)
                     /locus_tag="VHH53_06080"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX2289755.1"
                     /translation="MSRPDVVVHASAEVLAAAAAARLITRIVDIQSGSGSASLVLTGG
                     GIVIAALRQLASTPSAAAVDWRRLDIYWGDERFLPHGHPDRNETQAHEAL"
     gene            complement(278..1279)
                     /gene="opcA"
                     /locus_tag="VHH53_06085"
     CDS             complement(278..1279)
                     /gene="opcA"
                     /locus_tag="VHH53_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX2289756.1"
                     /translation="MIVDLPSTSTSAVNHALIDIRERGGAVALGRVLTLVIVTDDSAQ
                     VEESIQAANEASREHPCRILVVARGLKRAATRLDAQIRVGGDAGASEVLVLRLYGPLA
                     DHGDSVVIPLLLPDCPVVAWWPGVAPAVPSQDPIGKLAQRRITDAAAERHPIKALETR
                     RTSYTDGDTDLAWTRLTPWRALLAAALDLPPFEDIEAVTVTGGADNPSTDLLAGWLRS
                     RLQVPVRRRVSGPGGGISSAVLQRRGGPVELNRPDGKVGTLCQPGQPDRRVPLTRRPV
                     RDCLAEELRRLDPDEIYQAALAALTEVDGRAGTTHRRTAPAKKSRSNDILAESRSDQ"
     gene            complement(1276..>1727)
                     /locus_tag="VHH53_06090"
     CDS             complement(1276..>1727)
                     /locus_tag="VHH53_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX2289757.1"
                     /translation="GGEHGWYSFPLAWSVRGCIDQLVGGVGLRRGRRDPRRLHVGEAL
                     DFWRVEYLDRPRLLRLRAEMRLPGRAWLELEVIPDGDDGCRYRQRALFEPHGLAGHLY
                     WRTIAPFHGMIFGGMVRNITSAAEQHAGRASAPAVRGVSEGLAEGPQ"
BASE COUNT          270 a          615 c          587 g          255 t
ORIGIN      
        1 agcgcctcgt gagcctgagt ctcgttgcgg tccggatgac catggggcag gaaccgctcg
       61 tcaccccagt agatgtccag tcgccgccag tccactgcgg cagctgacgg ggtgctcgcc
      121 agttggcgca gcgccgcaat cacgatgcct ccgccggtca gcaccaggga cgcggaccct
      181 gagccggact gaatgtccac gatccgagtg atcagccggg cggccgccgc cgcggcgagc
      241 acctccgcac tggcatgcac caccacgtcg gggcggctca ttggtcgctc cggctttcgg
      301 ccaggatgtc gtttgatcgg ctcttcttcg ccggcgcagt gcgacggtgg gtggtgccgg
      361 ctcgaccgtc cacctccgtc aatgcagcca gcgcagcctg atagatctcg tccggatcca
      421 atctgcgcag ctcctcggcg aggcagtcac gcaccgggcg gcgcgtcaac ggcacgcggc
      481 gatccggctg acccggttgg cataaggtgc ccaccttccc gtcggggcgg ttcaactcca
      541 ccgggccgcc acggcgctgc agcacggccg aactaatccc tccgccggga ccgctgacgc
      601 ggcgccgaac cgggacctgc aggcgcgacc gcagccagcc ggcgagcaga tcggtggacg
      661 ggttgtctgc accgccggtg acggtgaccg cctcgatgtc ctcgaagggt ggcaggtcaa
      721 gtgctgcggc gagcagtgcg cgccacggcg tgagccgggt ccacgccagg tcggtgtccc
      781 cgtcggtgta ggaggtgcgg cgggtctcca atgccttgat cgggtggcgc tctgcggcgg
      841 cgtcggtgat ccggcgctgc gccagctttc cgatggggtc ctgcgaggga accgccggag
      901 ccacccccgg ccaccacgcg accaccgggc agtccggcag cagaagcggt atcaccacgc
      961 tgtctccatg atcggccagc ggaccgtaga ggcgcagcac cagaacctcg gaggcaccgg
     1021 cgtcgccccc gacgcggatc tgcgcatcca gccgcgttgc ggcccgtttg aggcccctgg
     1081 ccaccacgag tatccggcag ggatgctccc tgctggcttc gttggcagcc tgaatcgact
     1141 cctccacctg cgccgaatcg tcggtcacga tcaccagggt gagcacccgg cccagcgcta
     1201 ccgcgccgcc gcgctcccgg atgtcgatca acgcgtggtt cactgcggat gtgctcgtcg
     1261 agggcaggtc gacgatcact gtggaccctc agcaagtcct tcagacacgc cgcgcaccgc
     1321 cggagccgat gcgcggccgg cgtgctgctc ggcggcactg gtgatgttgc gaaccattcc
     1381 gccgaaaatc atgccgtgga agggggcgat agttctccag tacagatgtc cggccaggcc
     1441 gtgtggttcg aacagtgccc gctgccggta gcggcagccg tcgtcaccgt ccggaatgac
     1501 ctcgagttcc agccaggcgc ggccaggcag gcgcatctcg gcgcgcagcc gaagcagccg
     1561 aggccggtca aggtattcca cgcgccagaa gtccagagct tcgccgacgt gcaggcggcg
     1621 cgggtcgcgg cggccccggc gcaacccaac ccccccgacg agctggtcga tacagccgcg
     1681 cactgaccac gccagcggga aggagtacca gccgtgctcg ccgccga
//
LOCUS       DATADN010000469         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_659323, whole genome shotgun sequence.
ACCESSION   DATADN010000469 DATADN010000000
VERSION     DATADN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659323"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1479)
                     /locus_tag="VHH53_06095"
     CDS             complement(142..1479)
                     /locus_tag="VHH53_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2289758.1"
                     /translation="MSSTENRALDPDAASAAFKRRFMIRLVAVLLGGMFLDGYILGII
                     GPVSDTLSEELGISSLWEGLMAAAALLGILIGAPLGGWAADKFGRKPLFLADIMLFLV
                     ASAAQFFVDGPAQLFWVRLAMGIAIGAEYSVGWPLMSEFAPAHLRGRLMGLTLIAWYT
                     GFMVAYFIGFQLNAAGVSWRLILGSSTIIAVVLIFARLGLPESPRWLWSVNRHDEARA
                     VAHRYMANAADMVDVEHEDTQKGTFGMLFSRDYWRATLFMSVFWFCAVAPYFAIATFA
                     DSVLQQYGLAGGLAGGVGLSAVALAGVVLTVALIDKIGRRALTVPPQWLCTVVLAIIG
                     VWVGAPALVVLVLFLVFSFFNAMYNTLTGIYPGEVFPTEIRGIGTGFAAAVSRLGAAA
                     GTFLLPWSIDNLGIGPSMLIAAVVAAIGAGVSQWLAPETKGKSLTEAAHTFAH"
     gene            complement(1631..>1816)
                     /locus_tag="VHH53_06100"
     CDS             complement(1631..>1816)
                     /locus_tag="VHH53_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-pyrroline-5-carboxylate dehydrogenase"
                     /protein_id="HEX2289759.1"
                     /translation="VNDKPTGALMGQVSFGGGRASGTNDKTGSRLSLQRWISGRFVKE
                     TLAPATDWTYPYLDRSF"
BASE COUNT          335 a          644 c          592 g          245 t
ORIGIN      
        1 ccacgacagg ccaggcggtg cgtgccccga tgggcgccac ctgaggtggc cctcccggcg
       61 caggtctgcc cgattcgctc gaccacctcg tcgtcgcacg gcccatggca cgcctccagt
      121 gacttctgcc gtcgacgatc cctagtgcgc gaaggtgtgg gctgcctcgg tgaggctctt
      181 gcccttggtc tcgggggcca gccactgcga caccccggcg ccgatggcgg cgacgaccgc
      241 ggcgatcagc atggacggtc cgatcccgag gttgtcgatc gaccacggca gcaggaacgt
      301 gcccgcggcg gcgcccagcc ggctcaccgc ggcggcgaac ccggtgccga tcccgcggat
      361 ctcggtcggg aagacctcac cggggtagat gccggtcaac gtgttgtaca tcgcgttgaa
      421 gaacgagaag accaggaaca ggaccagcac gacgagcgcc ggggcgccca cccacacgcc
      481 gatgatcgcc aggaccacgg tgcacaacca ctgcggcggc accgtcagcg cgcggcgtcc
      541 gatcttgtcg atcaacgcga cggtcagcac gaccccggcc agcgcgaccg cggacagccc
      601 gaccccaccg gcgagcccac cggccaggcc gtactgctgc aacacgctgt cggcgaacgt
      661 ggcgatcgcg aagtacggcg ccaccgcgca gaaccagaac accgacatga acagcgtcgc
      721 ccgccagtaa tcccgcgaga acagcatccc gaaggtgccc ttctgggtgt cctcgtgctc
      781 gacgtcgacc atgtcggcgg cgttcgccat gtaccggtgg gccaccgcgc gggcttcgtc
      841 gtgccggttc acgctccaca gccagcgcgg cgactccggc aggcccaacc gagcgaagat
      901 caggaccact gcgatgatcg tgctcgagcc caggatcagg cgccagctca ccccggcggc
      961 gttgagctgg aagccgatga agtaggccac catgaagccg gtgtaccaag cgatcagggt
     1021 cagccccatc agccggccac gcaggtgggc aggagcgaac tccgacatca gcggccagcc
     1081 caccgagtac tcggcgccga tcgcgatacc catcgccagc cgcacccaga acaactgcgc
     1141 cggcccgtcg acgaagaact gcgcagctga cgcgaccagg aacagcatga tgtcggcgag
     1201 gaacagcggc ttacggccga acttgtccgc cgcccagcct cccagcggcg cgccgatgag
     1261 gatgccgagc agcgcggccg ccgccatcag gccctcccac agcgacgaga tacccagctc
     1321 ctcgctcagc gtgtccgaca ccggaccgat gatcccgagg atgtagccat ccaggaacat
     1381 cccgcccagc agcacggcga ccagccggat catgaaacgg cgcttgaacg ccgccgaggc
     1441 cgcatccgga tcgagggctc ggttctccgt cgatgacatc agtcactcct cacggtcaga
     1501 ttcgctcatt tgcccgaacg ggcggcgctg cgtcggtgtg catgggatgt ccctggggtg
     1561 tggagtccgg tgtgatctgg cctctggatg agcgggggcc aggcaacgac ccgaatcgtc
     1621 aggaccaccg tcagaaggag cggtccaggt acgggtaggt ccagtcggtg gccggagcca
     1681 atgtctcctt cacgaatcga ccgctgatcc accgctgcag ggagaggcgc gagccggtct
     1741 tgtcgttggt gccggaagcc cggccgcccc cgaaggagac ctggcccatg agagctccgg
     1801 tcggcttgtc gttgac
//
LOCUS       DATADN010000470         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1118549, whole genome shotgun sequence.
ACCESSION   DATADN010000470 DATADN010000000
VERSION     DATADN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118549"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1552
                     /locus_tag="VHH53_06105"
     CDS             260..1552
                     /locus_tag="VHH53_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012751064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2289760.1"
                     /translation="MLDAGLLDDVMDRVDAGGLRLTGEGGFLPEMIKAVLERGLAVEQ
                     TEHLGYEKGDVAGRGSPNSRNGTTPKTLATEVGDVRLDVPRDRAGTFEPRLVPKGSRR
                     AGGLDDMIVSLYAGGMTVRDISHHLARTLGTELSHDTISKVTDSVLEEVKAWQSRPLE
                     EIYPIIYLDALVVKVRDGHQVRNRSAHIAVGMDLDGVKHVLGIWVQASEGAKFWAGVC
                     AELRNRGVRDVLIVCCDGLTGFPEAIEATWPAATVQTCTVHLIRAAMRFVSYADRKRV
                     AAALRPIYTAPTVEAAETELLAFAESELGRRYPAAVATWDNAWERFIPFLAFAPELRK
                     IIYTTNAIESLNYQLRKIIKNRGHFPTDDAVIKLLWLAIRDIEDKRARQRTKEKGLPA
                     AQRKAPARLVEGAVVQGWKQALGALALAYPDRLAPYLT"
     gene            1605..>2779
                     /locus_tag="VHH53_06110"
     CDS             1605..>2779
                     /locus_tag="VHH53_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2289761.1"
                     /translation="MAQTLIPTIGHALGDGLNRTDLAVLERYADPRALLGAGRARLLR
                     LVRDESRGKLGDDKVQALRTAAEQALQLWGEDPAIALSDLAEEITTEIRLLRLAETER
                     ARHEAARDAALAHVDPDGLATSLPGLGPVEATQLLAAMERPGRFRHPATFKSFTGLTP
                     KASETGNTDRKSQPMSKAGNSALRTQLIQSANTARQLDPQLAAIYHAQMTQRGAPHLK
                     ALCVVAARLAERAWLTLSKAQPYVIRDLQGRPISPSQARELIAAQFTVPANVRRRRRS
                     NKLAGRGTLRPLRLETGGRSPSHPERSLHPSRTYPPVLTHRLPAMMQTCGSASSPTGS
                     YRFDRTTATTLWPIRLSRRPDVLRTPTFATRAAALKLSINAAEWRLTRGYASAKIRGG
                     "
BASE COUNT          535 a          965 c          840 g          439 t
ORIGIN      
        1 caccctggtc gaggaacgca cccgggtggt gcagcggttg gagaagatcc tgcaggacgc
       61 cgggatcaag ttgaccagcg tggcctcggc gctgctgacc aagtccgggc gggagcgtgt
      121 ctgatggtct gtgtaacaag atcctcttga gttgaggagt acacagatga ccgtgactga
      181 tgatcagcag tgggagcacc cgttgggcct ggtgccgtgc gcgccgtccc cgacctcggc
      241 gcgtgaggca atcaacgaga tgctcgacgc cgggttgctg gacgatgtga tggaccgggt
      301 cgatgccggc ggtttgcggt tgaccggcga gggtggcttc ctcccggaga tgatcaaagc
      361 cgtgctcgag cggggcctgg cggtggagca gaccgaacac ctgggttatg agaagggtga
      421 cgtcgccggg cgtggcagcc cgaactcacg caatggcacc accccgaaga ctttggcgac
      481 cgaggtcggt gatgtgcgcc tggacgtgcc gcgcgaccgt gccgggacct ttgagccccg
      541 gttggtcccg aaaggctctc ggcgcgccgg cggcctcgac gacatgatcg tctcgctgta
      601 tgcgggtggg atgacggtac gcgatatctc tcatcacctg gcccgcacgc tgggcaccga
      661 gctgtcccac gacaccattt ccaaggtcac cgactcggtg ctcgaggagg tcaaggcctg
      721 gcagtctcgg ccgttggagg agatctaccc gatcatctac ctggatgcgc tggtggtcaa
      781 agtccgcgac ggccaccagg tccgcaaccg ctccgcgcac atcgccgtcg ggatggatct
      841 cgacggggtc aaacacgtac tcgggatctg ggtgcaagca tccgaaggcg ccaagttctg
      901 ggccggggtg tgcgccgagc tgcgcaaccg cggggtgcgc gacgtgctca tcgtgtgctg
      961 cgatggcctg accgggtttc ccgaagccat cgaggccacc tggccggccg ccaccgtaca
     1021 aacctgcacg gtgcacctga tccgggcggc gatgcggttt gtgtcctatg ccgaccgcaa
     1081 gcgtgtcgcc gccgcgctgc gtcccatcta caccgccccg actgtcgaag ccgccgagac
     1141 cgagctgctg gccttcgccg agtccgagct gggccgccgc tacccggccg cggtcgcgac
     1201 ctgggacaac gcctgggagc ggttcatccc cttcctggcg ttcgcacccg agctccgcaa
     1261 aatcatctac accaccaacg cgatcgagtc gctcaactac caactccgga agatcatcaa
     1321 aaaccgcggt cacttcccga ccgatgacgc ggtcatcaag ctgctgtggc tggcaatccg
     1381 tgacatcgag gacaaacgcg cccgacaacg aaccaaggaa aagggcctac ccgcagccca
     1441 gcgcaaagcc cccgcgcgcc tcgtcgaagg cgccgttgtc caagggtgga aacaggccct
     1501 gggagccctc gcactggcct atcccgaccg ccttgccccc tacctcacct gaccacccat
     1561 agatcaactt acacagacaa cttgacaggc tccgcatccg cgcattggct cagacgctga
     1621 tccccaccat tggacacgca ctcggtgatg ggctcaaccg cacggacctg gccgtgctgg
     1681 aacgctatgc cgatcctcga gctttgctag gcgccgggcg agcacgattg ctacgactag
     1741 tccgtgacga aagccgcggc aaactcggtg acgacaaagt acaagccctg cgcactgccg
     1801 ctgagcaggc actgcagctg tggggtgagg accccgccat cgcattgtct gacctcgccg
     1861 aggagatcac caccgagatc cggttgctgc ggctggccga gaccgaacgc gcccgtcacg
     1921 aagccgcccg cgacgccgcg ctggcccacg tcgatccgga cggattggcc accagtctgc
     1981 ccggcctggg cccggtggaa gccacccaac tgctggccgc gatggaacgg ccaggtcgct
     2041 tccgccaccc cgcgacgttt aagtccttca ccggattgac gcccaaggcc agcgaaaccg
     2101 gcaacaccga tcgcaaaagc caaccgatga gcaaggccgg caacagcgct ctacgcaccc
     2161 agctaatcca gtccgccaac accgcccgcc aactcgatcc acaactggcc gcgatctacc
     2221 acgcacaaat gacccaacga ggcgcaccgc acctcaaagc cctgtgcgta gtcgctgcac
     2281 gtctcgccga acgcgcctgg ctcacactgt ccaaagcaca gccctacgtc atccgtgatc
     2341 tccaaggccg acccatcagc ccttcgcagg cccgcgagct catcgccgca cagttcaccg
     2401 tcccagccaa cgttcgccgt cgacgacggt caaacaagct agcgggaagg ggcaccctca
     2461 ggccgctgcg gctggagact ggcggtcggt caccgtcgca tccggagcgt tccctgcatc
     2521 catctcgaac ttacccaccg gtactcacac accgattgcc cgccatgatg cagacctgtg
     2581 gcagcgcctc gtcaccaacg ggaagctacc gattcgaccg gactacggcg actacgctgt
     2641 ggcccatccg gctatcccga cgcccggacg tgctccgaac cccaaccttc gctacacgcg
     2701 cagcagcgct caagctgtcg atcaatgctg ctgaatggcg tttgaccagg ggctatgcgt
     2761 ccgcgaagat acgtggagg
//
LOCUS       DATADN010000471         2622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1249598, whole genome shotgun sequence.
ACCESSION   DATADN010000471 DATADN010000000
VERSION     DATADN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249598"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VHH53_06115"
     CDS             complement(<1..1818)
                     /locus_tag="VHH53_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2289762.1"
                     /translation="MAAITGSRAAAAVGALFVVVVAVLTLLSERPPSPVPASAPAQQF
                     SAQRALVALQEFATEPRPLGSVASDRARDYLVDALRSAGFSVQTQRAVGSVSAEGVAA
                     FGRVDNIVATLPGRNPTGSVVLAAHYDSVAAGPGASDDGAAIAAMLETVRALRGGDQL
                     RNDLVLLITDGEEDGLLGAAAFADQHPLIRQRAVVLNWEARGVSGPSLMFETSRNNAA
                     LVGLFAEAAPHPHGDSSSIEFYRLLPNNTDFTEFAGAGLSGMNFAYIEGAARYHTPGD
                     SIANLDPASLQHHGANMLGLTRALGNSDLTTLTADHDVIFFRFLGPLIIYPLALVWPL
                     ALLVLIVLAGVVVLARHRRLVSIPRLLLSAISVVLPLATAAVAVQGLWRVLVALRPDY
                     ADNPFLYRPQLYQLAAAALAALAVLTWYLLLRHRLGSAALACGSLVWLALLSVLSAWV
                     APATSHLVTLPALFLAGGGLVALLVSRRAVWPVVAITVGVVPSVVLLMPMGVATFDAA
                     GLPGGGAAAVLLALFGLTLLPLVELLLPPVEQPLGTRRAALVAIAGTGLVLAVTGAGL
                     AVDPVDVAHPRRAHLAYVLDTDTGLARWVSRDTEPAEWTR"
     gene            1940..2305
                     /locus_tag="VHH53_06120"
                     /pseudo
     CDS             1940..2305
                     /locus_tag="VHH53_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019811201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
BASE COUNT          481 a          896 c          905 g          340 t
ORIGIN      
        1 gcgagtccat tcggctggtt cggtatcccg gctcacccag cgggccagac cggtgtcggt
       61 atcgagcaca taggccaggt gcgcgcggcg tggatgggcc acatcaaccg ggtccacggc
      121 caggccggcg ccggtcacgg cgagcaccag gcccgttccg gcaatcgcaa ccagcgcggc
      181 gcggcgcgtg ccgaggggct gctctaccgg tggaaggagc aactcgacca gcggtagcag
      241 ggtgagcccg aacagggcca gcagcaccgc ggccgccccg ccccctggca gtcctgcggc
      301 gtcgaaggtg gccacaccca tcggcatcag cagcactacg gacgggacga caccgacggt
      361 tattgctacc accggccaca ccgcacgtcg tgataccagc agcgcgacca gcccgccacc
      421 ggccaggaac aacgcgggca acgtgactag gtgcgatgtt gcgggtgcga cccaggcact
      481 gagtacgctg agcagcgcta gccagaccag cgagccgcag gccagcgcgg ccgagccgag
      541 ccggtgccgc agcagcaggt accaggtcag caccgcgagt gcggccagcg cagcggcagc
      601 caactggtag agctgcgggc gataaaggaa cgggttatcc gcgtagtcgg gtcgcagcgc
      661 caccaagacc cgccacaggc cctggaccgc cacggcggcg gtggccagcg gcaacaccac
      721 cgagatggcg ctgagcagca gtcggggaat gctcaccagc cgccgatgcc gggccagcac
      781 gaccacaccg gcgagcacga tcagcacgag cagcgccagc ggccacacta gagcgagtgg
      841 atagatgatc aagggtccta ggaagcggaa aaagatcaca tcgtggtcgg cggtcagggt
      901 ggtcagatcg ctgtttccca gcgcgcgggt gagcccgagc atgttcgcgc cgtggtgctg
      961 caggctggcc gggtcgagat tggcgatgct gtcacctggg gtgtggtagc gcgccgcgcc
     1021 ctcgatgtag gcgaagttca tgccggacag gccagccccg gcgaactcgg tgaagtcagt
     1081 gttgttgggc agcagccggt aaaactcgat cgacgaggaa tccccgtgtg ggtgcggtgc
     1141 ggcctcggcg aacaaaccga ccagcgctgc gttgttccgc gacgtctcga acatcaacga
     1201 agggccacta accccgcgag cctcccagtt gagcacgacc gcccgctgcc ggatcagtgg
     1261 atgctggtcg gcaaaggcgg cagcgccgag caggccgtcc tcctcgccat cggtgatcag
     1321 gagtaccagg tcgttgcgca gctgatcacc accgcgcagc gcgcgcaccg tctccagcat
     1381 cgcggcgatc gcggccccgt cgtcggaggc acccggaccg gccgcgaccg agtcatagtg
     1441 cgcggcgagc acaacgctgc cggtcgggtt gcgcccgggc agggtggcga cgatgttgtc
     1501 cacccggccg aaggccgcga cgccctcggc gctgactgac ccgaccgcgc gctgggtctg
     1561 caccgagaaa cctgccgagc gcagtgcatc gaccaggtag tcccgggcgc gatcgctggc
     1621 cacgctgccc agcgggcgag gctcggtggc gaactcctgc aaggccacca gcgcccgctg
     1681 cgcactgaac tgctgggccg gcgccgatgc gggtaccggg ctcggcggcc gttcggacag
     1741 cagcgtgagg accgcgacca cgacgacgaa cagcgcgccc accgcggccg ccgcgcggct
     1801 acccgtgatg gctgccaacg caggccgcca tgccgatcga gagattcatg ttcgcgacgg
     1861 taaccgcaag ccccgacatc tccaggcgac ggatacgctt cgactcttgc gagtgcggcg
     1921 tccgaggagc acgacaagca tgatcaccag gctgtcgacg ttgttcctgc gcaccttgcg
     1981 cgaggatccg gccgacgccg aggtgcccag tcacaagctg ctggtgcgtg ccggatacgt
     2041 ccgccgggtc gctccggggg tctactcgtg gctgccgctc ggactacggg tactgcgcaa
     2101 tatcgaggca gtggtgcgtc aggagatgga cgcgatcggc gcgcaggaga tccagttccc
     2161 tgcgctgctg ccccgggagc cctaccaggc caccggccgc tggaccgagt acggcgccaa
     2221 cgtgtttcgg ctgcacgacc gcaagggtgc cgactacctg ctcggcccca cccacgagga
     2281 gctgttcacg ctcaccgtca aggggccgcg agcagcgccg agaaaagcgc caccacgagc
     2341 gcccagctca acaacgagac gccccgcgca atgctagagg cggcagcaaa gccaccgccg
     2401 agagcagcac ccggtgctcg gcaagagtac gatagctgaa atgcgacggc tggtgcctga
     2461 tcgctaaatc agcggagggc accggcgtag agccgctccc aggcctccat ctccggggtg
     2521 aagtagcgca cccgcgtctt cttgtactcg cgggtggagt agagcgagag ccgctcggtg
     2581 atgccgctct cggcggccat cgcgtccagg acgctctcgc ac
//
LOCUS       DATADN010000472         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1118699, whole genome shotgun sequence.
ACCESSION   DATADN010000472 DATADN010000000
VERSION     DATADN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118699"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /gene="rpsI"
                     /locus_tag="VHH53_06125"
     CDS             <1..117
                     /gene="rpsI"
                     /locus_tag="VHH53_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX2289763.1"
                     /translation="DDRPALKKAGFLTRDARVKERKKYGLKKARKAPQYSKR"
     gene            144..1499
                     /gene="glmM"
                     /locus_tag="VHH53_06130"
     CDS             144..1499
                     /gene="glmM"
                     /locus_tag="VHH53_06130"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX2289764.1"
                     /translation="MSAPPALKPRLFGTDGVRGLANFDLTPELALAVSGAAARVLGTQ
                     GLGHRPVAVVGRDPRASGEMLEAAVTAGLTSAGAEVLRVGVLPTPAVAYLVAELSADL
                     GVMISASHNPMPDNGIKLFAAGGHKLPDAAEDEIIAAMDSCDGQRPTGARIGRVHEVP
                     EATGRYVDHLLLATPHPLEGVRVVVDCAHGAASVIAPEAYRRAGADVVALHAEPNGLN
                     INDGCGCTHLDPLRKAVLAHGAAIGIAHDGDADRCLAVDASGDVVDGDQIMAILALAM
                     RDNGELAQDTLITTVMSNLGLHLAMRQHGVVVQTTAVGDRYVLEALWAGGFSLGGEQS
                     GHVVLPEYATTGDGLLTALRLMAQMIASGQSLADLAGVVTRLPQVLVNVAVGDKAAVA
                     AAPSVVDAIAAAEVELGETGRVLLRPSGTEQLVRVMVEAPTQLAAETIAHRIAAVVAG
                     I"
     gene            complement(1500..>1980)
                     /locus_tag="VHH53_06135"
     CDS             complement(1500..>1980)
                     /locus_tag="VHH53_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2289765.1"
                     /translation="ALDLCVGVRLGNGEISVDPGKLALAGHGMGGGCAVLAAATDDRC
                     QAVVTLALAETMPSAVAAAPECTMPGMHLAAGKDRVAPPIGHAQPVATAWGGPVLLRT
                     LPNASHLGFLHDQHWTDWLVDGSPEYSTRRATQPLVTAFLLRHLTGAARYDTLLEEP"
BASE COUNT          301 a          630 c          728 g          321 t
ORIGIN      
        1 gatgaccgtc cggcgctgaa gaaggcaggc ttcctcaccc gtgacgcgcg ggtcaaggag
       61 cgcaagaagt acgggttgaa gaaggcgcgc aaggcgcccc agtactcgaa gcgctagtac
      121 tcgaagcgtt tgtgcgtgac gcgttgagcg ccccgcctgc gttgaaaccc cgcctgttcg
      181 gcaccgacgg ggtgcgcggg cttgccaact tcgacctcac gcccgagctg gcgctggccg
      241 tatccggtgc agcggcgagg gtgctcggga cgcagggact cgggcatcgg ccggtcgccg
      301 tggtggggcg ggacccacgg gccagcggtg aaatgttgga ggcggccgtc accgcgggtc
      361 tgacctcggc gggtgccgag gtactccggg tcggggtgct gcccacccca gcggtcgctt
      421 acctggtggc cgagctctca gcagaccttg gtgtgatgat ctcggcctcg cacaacccga
      481 tgccggataa cggcatcaag ctgtttgccg ctggcgggca caaactgcct gatgcggcgg
      541 aggacgagat tatcgccgcg atggacagct gcgatgggca gcgcccgacc ggcgcccgga
      601 tcggtcgggt gcatgaggtg ccggaggcca ctgggcgcta tgtggatcac cttcttctgg
      661 ccaccccgca tccgctcgaa ggggtccgcg tggtggtgga ctgcgcgcac ggtgcagcct
      721 ccgtaatcgc acccgaggcc taccgaaggg ccggtgccga cgtggtggcg ctgcacgccg
      781 agcccaatgg tctcaatatc aacgacggct gcggatgtac tcacctggat ccgttgcgca
      841 aggcggtgct ggcccacggc gctgcgatcg gtatcgcgca tgacggcgac gcggaccgtt
      901 gcctggccgt ggacgcctcc ggcgacgtgg tggatggcga ccagatcatg gcgatcctcg
      961 cgctggccat gagggacaac ggtgagctgg cgcaggacac gctgatcacc acggtgatga
     1021 gcaatcttgg gttgcacctg gcgatgcgcc agcacggggt ggtggtgcag accacggcgg
     1081 taggtgaccg gtacgtgctc gaggccctgt gggcgggcgg gttctcattg ggaggtgaac
     1141 agtccgggca tgtggtgctg ccggagtatg ccaccaccgg cgacgggctg ctcaccgcgt
     1201 tgcgcttgat ggctcagatg atcgcctcgg ggcagagcct ggcggatctg gccggcgtgg
     1261 tcacccggtt gccgcaggtg ttggtcaacg ttgcggtggg cgacaaggca gcggtcgcgg
     1321 cggcaccgtc ggtggtcgac gccatcgccg cggcggaggt tgaactgggt gagaccggcc
     1381 gggtgttgtt gcgcccttca ggcaccgagc agctggtccg ggtgatggtc gaggccccca
     1441 cccagctcgc cgccgaaacc atcgcccacc gcatcgctgc agtcgtcgct ggaatctgat
     1501 cagggttctt ccagcagggt gtcgtagcgg gcggcgccgg tgaggtggcg cagcaggaat
     1561 gcggtgacca ggggttgggt agctcgccgg gtgctgtact ccgggctgcc gtcaacgagc
     1621 caatccgtcc agtgctgatc gtgcaagaag ccgaggtggc tggcgttggg cagcgttcgc
     1681 agcagtaccg gaccccccca cgctgtggcc accggctgcg catgcccgat cggcggggcc
     1741 acccgatcct tgccagcggc caggtgcatg ccgggcatgg tgcactcggg tgcggcggcc
     1801 actgctgacg gcatggtttc ggccagcgcc agggtcacca cggcctggca acggtcatcg
     1861 gtcgccgcag cgagcactgc acagccgccc cccatgccgt gcccggccag ggcgagcttg
     1921 cccggatcca cactgatctc gccgttgccc agccgcaccc cgacgcacaa gtccagcgca
//
LOCUS       DATADN010000473         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_798934, whole genome shotgun sequence.
ACCESSION   DATADN010000473 DATADN010000000
VERSION     DATADN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798934"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VHH53_06140"
     CDS             <1..715
                     /locus_tag="VHH53_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2289766.1"
                     /translation="SGSGLAVVALHGGPIARYGPGLVPEFQLFARLGLPVVALNYPGS
                     TGSGHEYTRSLFGQAGAIDVEAVTSVIDGLRAQGREVILYGESYGAFLALSAAAVRPC
                     AGVIAFAPFASFESLSASGSPEVRDLLDLVGGGNQEAYGRNLLTACRTIRGKVLISHG
                     TADIRIPVAESWTLVQALRGRDGAGEHDTQFVAMDGQGHDLSGRPVLQRWYREIATFV
                     VDLPCSRTPAQREHQQQGR"
     gene            759..935
                     /locus_tag="VHH53_06145"
     CDS             759..935
                     /locus_tag="VHH53_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289767.1"
                     /translation="MFELTTGADALGLQSVAETAQLGRRHVRKYRKNVCLITLNGSLN
                     GLGILNILNGFGGF"
     gene            1066..1257
                     /locus_tag="VHH53_06150"
     CDS             1066..1257
                     /locus_tag="VHH53_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289768.1"
                     /translation="MFELIGADVLGLQSVAETAQLRRRGGGHGGSKSSYRKNVCLITL
                     NGSANGLSILNILNGFGGF"
     gene            1446..>2001
                     /locus_tag="VHH53_06155"
     CDS             1446..>2001
                     /locus_tag="VHH53_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289769.1"
                     /translation="MPTLVRMGWRADRGGTAALFLAGIFKAAATIGATVTIGLLVDAI
                     VKNGGTSGSAPWIALGTVAAIFLIQRVAFPFLGPAIESLEHRLTILVQQRVMAPLLRP
                     VTIGHLEDPDLADELRLAQQVGSENFSAQQALGALNDLVSTRLAATAAGVILFTWRWW
                     APVALLGAWLVSRLWYRIQMATLVT"
BASE COUNT          302 a          698 c          637 g          364 t
ORIGIN      
        1 ctcggggtca ggcctggcgg tggtggctct ccacggaggg cccatcgccc gctacggccc
       61 cggtctggtg ccggagttcc aactcttcgc ccggctgggg ctgcccgtcg tcgcactcaa
      121 ctatcccggc agcaccggat cggggcacga gtacacgcgc tccctgtttg gccaggccgg
      181 tgccatcgac gtggaggccg tcacgtcggt catcgacggg ctccgcgccc agggccgcga
      241 ggtcattctc tacggcgaga gctacggggc cttcctggcc ctctcagcgg ccgctgtgcg
      301 gccgtgtgcc ggtgtgatcg ccttcgcgcc gttcgcttcc ttcgagagcc tcagcgcctc
      361 cggctcgccg gaggtgcgtg accttctcga cttggtcggg gggggaaatc aggaggcata
      421 cgggaggaat ctgctcacgg cctgccgaac catacggggc aaggtgctca tctctcatgg
      481 caccgcggat attcggattc ccgtagcaga gtcctggacc ctggtccagg cgctgcgggg
      541 cagagatgga gctggggaac acgacacgca gttcgtcgca atggacggcc agggtcatga
      601 cctgtccggt cgtccggtcc tccagcgctg gtatcgggag atcgccacgt tcgtggtgga
      661 ccttccgtgt tcgagaacgc ctgcccaacg agaacaccag cagcaggggc gttgagcagg
      721 cggcctcggg aagaccaacc cgaaaggagg tgaacaatat gttcgagctc accaccggtg
      781 cggacgctct tggtctgcag tcggttgccg agacggctca gctgggccgg cgtcacgtgc
      841 gcaagtaccg caagaacgtg tgcctcatca cgctcaacgg ttcgctgaac ggcctcggca
      901 tcctgaacat cctgaacggc ttcggcggtt tctaagtcgc caaccacgtt tccaattttc
      961 gacgggcggc acagggtccc cataggaggg acctgtgcct cccgatcgat acctcgctcg
     1021 ggacgctgag cgactactcg cattgtgcga aaggggatcc accaaatgtt cgagctcatc
     1081 ggtgccgacg tcctcggcct gcagtcggtt gctgagaccg cccagcttcg tcggcgtggc
     1141 ggcggccacg gtggtagcaa gtccagctac cgcaagaacg tctgcctcat cacgctgaac
     1201 ggctccgcca acggcctgtc gatcctgaac atcctcaacg ggttcggcgg cttctaagca
     1261 gcctcactgc cggcctggcg ccgggcctac gggtccgtgc gccagccggc ttttttttcg
     1321 cgtttgggga cattcgctgc tccacctgcg gaccggcgca ggactgaagg gccacctcct
     1381 tgaaagaacc ccccatggac gcacctcgcc cccgaggcgt gcgcgccgct ctcgcgggcc
     1441 gcccagtgcc gacgctcgtc cgcatgggct ggcgtgctga ccgtggcggc accgctgctc
     1501 tctttctggc cggaatcttc aaggccgccg ccaccatcgg cgccaccgtc accatcgggc
     1561 ttctggtcga cgccatcgtc aagaacggcg gcaccagtgg ttccgcaccc tggatcgccc
     1621 tcggcaccgt ggctgccatc ttcctcatcc agcgagtcgc cttcccgttc cttggcccgg
     1681 cgatcgagag cctcgaacac cggctcacga tcctcgtcca gcagcgggtc atggcgccgc
     1741 tgctccgccc ggtgaccatc ggccaccttg aagacccgga cctggccgac gaactgcggc
     1801 tggcccagca ggtcggctcg gagaacttct ccgctcagca ggccctgggc gcgctcaacg
     1861 atctggtgag cacgcggctg gccgccacgg ccgccggcgt cattctcttc acctggcgct
     1921 ggtgggctcc ggtcgccctc ctcggcgcct ggctcgtctc gcggctctgg taccgcatcc
     1981 agatggcgac gctggtcaca t
//
LOCUS       DATADN010000474         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_196790, whole genome shotgun sequence.
ACCESSION   DATADN010000474 DATADN010000000
VERSION     DATADN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196790"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VHH53_06160"
     CDS             <1..468
                     /locus_tag="VHH53_06160"
                     /inference="COORDINATES: protein motif:HMM:NF039955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HEX2289770.1"
                     /translation="VTRVAYNPDLRGPAPQRLDADGRTIRLGWFRNLDRQLLNMTGDS
                     SCGRVDFLVVPADTTAAAAEHAFSAATGRANRQAPTELLDTLSAAGAPVPLPRSADQP
                     DTEETAAWAPKGPPVPLTPGPTTPLPARGLVHGHNERPSPIEHLPSTVGAVLG"
     gene            complement(767..1156)
                     /locus_tag="VHH53_06165"
     CDS             complement(767..1156)
                     /locus_tag="VHH53_06165"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2289771.1"
                     /translation="METITDTGTLPDPATLAARRRLIVSAALVGASCDASPPGAVGRR
                     HRALARRLDDYLRFAGDLRVPFDNNAAERDIRMAKIKQEVSGCMRTLAGALDFVALRS
                     CRATAAKHGRRPFDVLTELVSVNAGSQ"
     gene            1205..1521
                     /locus_tag="VHH53_06170"
                     /pseudo
     CDS             1205..1521
                     /locus_tag="VHH53_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1865..2338
                     /locus_tag="VHH53_06175"
     CDS             1865..2338
                     /locus_tag="VHH53_06175"
                     /inference="COORDINATES: protein motif:HMM:NF020332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HEX2289772.1"
                     /translation="MEAGRGRADDYYLAEGTGIARRYAAAGGGRVQELAALAGDDYEA
                     WVAGLDPDTGTARGRLRTDERAVRFVEVVVNGPKSWSLAAALHPTRRGGGTTRADSAR
                     SPSARRATLIVTRPRPRRTRHAARTSNPRRPPSPSTPGGGRRPAHTDRPRRGGPR"
     gene            2392..>2629
                     /locus_tag="VHH53_06180"
     CDS             2392..>2629
                     /locus_tag="VHH53_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX2289773.1"
                     /translation="MRPSEVQAWATDRSRVLAPTTLRQAVGLLRSVYGAAVLDRLVAS
                     SPVVRIQLPRHERERIVPLSVEQVAALARAMPERY"
BASE COUNT          442 a          915 c          883 g          389 t
ORIGIN      
        1 gtcacgcggg tggcttacaa ccccgacctc cgggggcccg cgccgcagcg gctggacgcg
       61 gacggccgga ccatccgcct cggctggttc cgaaacctcg accggcagct gctgaacatg
      121 accggtgact cgagctgcgg ccgggtggac ttcctcgtgg ttcccgcgga cacgacggcg
      181 gccgctgcgg agcatgcctt ctcggcggcc accggccggg ccaaccggca ggcacccacc
      241 gagctgctcg acacgctcag cgccgccggc gcaccggtac cactcccacg ctccgccgac
      301 cagcccgaca ccgaggagac cgcggcctgg gctccgaagg ggccacctgt accgctgacg
      361 ccgggcccaa ctacgccact cccggccagg ggtctagttc acggacacaa tgagagacca
      421 tcaccgatag agcaccttcc ctccacagtg ggagcagtac tcggatgagg cagttgctat
      481 ctgccttccg caactgcagc agtaaggttc cgcgttgtcc ggtgtattcc ctgagcgcgg
      541 cacgccacac accgagcagt acttagacgt tacagtcgaa tattcttcac cgcagccggg
      601 gcagtaaccg ggtcgtagcg cactcggggt atatggacga tccgttcccg gcgtgccaca
      661 ctggggacag aatcttgtga agcctgactt gtgtccacac tggtcgcagt atgtatgtcg
      721 tacctctgcc atggtgcact gtagcctgac gtaactgttc agctcgtcac tgggatcctg
      781 cgtttacgct aaccagttcg gttaggacgt cgaagggtcg gcggccgtgc ttggcggcgg
      841 tggctcggca ggagcgcaag gcgacgaagt ccagggcgcc ggcgagggtc cgcatgcatc
      901 cggacacctc ctgcttgatc ttcgccatgc ggatgtccct ttccgcggcg ttgttgtcaa
      961 aaggaactcg caggtcgccg gcgaagcgca ggtagtcatc gaggcggcgg gccagggcgc
     1021 gatggcggcg gccgaccgcc ccgggcgggc tggcgtcgca cgaggcgccg accagggctg
     1081 cggagacgat cagccgtcgg cgcgcagcca gcgtggcagg gtccggcagc gttccggtgt
     1141 cggtgatcgt ctccaggcca acagcgcagg tggtgtcctg gcggacgccc gacccctacc
     1201 gcacgtcacc gacctgctca ccggcctgcg cgaccgcggc ctggacatca cccgcccatc
     1261 ctggtggtca tcgacgaggc aaaggcgcta cgccgggcgg tgctcgacgt ctttgaccac
     1321 ccggtgctcg gtcgctgcca gctgcacaag atccgcaacg tgcgcgaccg gttgcccgag
     1381 cggctgcgct cgaccgtggc caagcggatg cgcgccacct accacgccga ctcggtcctc
     1441 gacgccgaag cacagctcac cgcgctggcc cgcgagctgg acaagaccca cccgggcgcg
     1501 gccgccagcc tgcccgaggg agtcgggacc gcctcggacc tcgtctacgc ccttccggtg
     1561 tcgcccaaga tcaacatgga cacacctcct gcacctgcga gcccacaatc cagcgatcac
     1621 caacaccatt tggaagcgcg acccgcgcgt tatgagaaat ccactacagc cagtcctggt
     1681 cataatcacc caagccttca ccagaagacg gcacatatgc taccaatatt ggcagtccgg
     1741 gctccgcttc gcggggacca gtgccgtttc tgggggtggc gtgttggtca ttgtttgatg
     1801 gcgttgataa atgcacggcg gaatgaagat ctacgctggc gctccttcgc cggcccggca
     1861 ctacgtggag gccggccggg gccgggccga tgactactac ctcgccgagg ggaccgggat
     1921 cgcgcgccgc tatgcggccg ccggcggcgg cagagtgcag gaactcgccg cgctggccgg
     1981 ggacgactac gaggcgtggg tggccggcct ggaccccgac accggaacgg cccgggggcg
     2041 gctgcggacc gacgagcggg cggtgcggtt cgtcgaggtg gtggtcaacg gcccgaagtc
     2101 ctggtcgctg gccgccgccc tgcaccctac gaggcgcggt ggcgggacga ccagggcaga
     2161 cagcgcaaga agtccttcag caagaagggc gacgctgatc gtcacgcggc cacggccgag
     2221 gcggacaagg cacgcggcac gtacgtcgaa ccctcgaaga ccaccgtcgc cgagtacgcc
     2281 cggaggtggg cggagacccg cccacaccga ccgaccacgg cgcggcggac cgagatgatg
     2341 atccgactcc acatcgacgg aacgcgtctc ggtggccgga ggctcgcgga cgtgcgcccg
     2401 tccgaggtcc aggcgtgggc caccgaccgg tcgagggtgc ttgccccaac caccctgcga
     2461 caagccgtcg ggctgctccg gagcgtgtac ggggccgcgg tcctcgaccg actggtggcc
     2521 tcctcgcccg tcgtccgcat ccagcttccc cgacacgaga gggagcgaat cgtgccgttg
     2581 tcggtcgagc aggtagcggc gctcgcccgg gccatgccgg agcgctacc
//
LOCUS       DATADN010000475         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1418939, whole genome shotgun sequence.
ACCESSION   DATADN010000475 DATADN010000000
VERSION     DATADN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418939"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VHH53_06185"
     CDS             <1..517
                     /locus_tag="VHH53_06185"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX2289774.1"
                     /translation="PAAQEMVRQAAAQADLVLVTIHAGAEGADRGQVRPGTEIFLGED
                     RGDAVAFSHAVIDAGADAVLGAGPHVLRGMEWYRGRLIAYSMGNFLGYQTLSHTGPQG
                     VGGIVTLQLMPDGSWHGGQLQGTVMVDPGVPQLDPDQRARARVQELSRTDFGACGVQV
                     SAAGELNTPTC"
     gene            994..1416
                     /locus_tag="VHH53_06190"
     CDS             994..1416
                     /locus_tag="VHH53_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2289775.1"
                     /translation="MGEARARLEQNIQHWNAHDSAAWVGDFSPTAQIVGPGASGSGIE
                     MARTFYSIWQDAFPDNEVRVTDIFEDGATAILQAEFRGTQTETMNAPEQSIPATGRRV
                     NIPFVLINRFDGGKTTAFTLYFDRAELLGQLGLAPAPS"
     gene            complement(1580..2128)
                     /locus_tag="VHH53_06195"
     CDS             complement(1580..2128)
                     /locus_tag="VHH53_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2289776.1"
                     /translation="MRRPAAHKAGRQRFVSGKARANTVKALVITDLAGRLLFCGQTLP
                     GAVHDLTQVRQAGLVELLALTPGVTLLADPGYQGLSTQTAGAVITPRPARRKNQLPVP
                     PALAAAHEAECRAHASKRIRVEHGISHLKNWRALSRHLGRREHLETMLPAVAGLVSSQ
                     ERAPRPEPLHRSPKASPAGTAA"
     gene            complement(2044..2553)
                     /locus_tag="VHH53_06200"
     CDS             complement(2044..2553)
                     /locus_tag="VHH53_06200"
                     /inference="COORDINATES: protein motif:HMM:NF025000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2289777.1"
                     /translation="MSRTLARAAAVADRLVTGLSREVLAELVAELGPRWQARQDARLA
                     DRPRRRAVGAGARYRLVFLDRLLATLVHLRHGVTHDVLACWCGVSRSTITRAIGELRP
                     LLAERGCTVEGGLRLRTLADVVAHLGASRQLGLLNATEVRCAARQPTKPVASGSSRAR
                     PGPTRSRRW"
BASE COUNT          421 a          960 c          919 g          401 t
ORIGIN      
        1 tcccgccgcc caagaaatgg tgcgacaggc tgccgcccag gccgatctgg tcctggtcac
       61 catccacgcc ggcgccgagg gcgccgaccg cgggcaggtg cggccgggca ccgagatctt
      121 cctcggcgag gaccgcggtg acgcggtcgc gttctcccac gcggtgatcg atgccggcgc
      181 cgatgcggtg ctcggtgccg gtccgcatgt gctgcggggc atggagtggt accgaggtcg
      241 cctgatcgcc tacagcatgg gcaacttcct gggctaccag acgctgtctc acaccgggcc
      301 acagggggtg ggcggcatcg tgacgctgca gctgatgccc gatggcagct ggcacggcgg
      361 gcagctgcag ggcaccgtca tggtcgaccc gggagtgccg cagctcgatc ccgaccagcg
      421 cgcccgcgca cgcgtgcagg agctgtcccg caccgacttc ggcgcctgcg gcgtgcaggt
      481 ctccgccgcc ggtgaactga acacccccac ctgctgacac cccgcgcccg gcccggcgga
      541 acaatcagcc gccggtcccg tccgccctcg ccgccgtcca cgaggccgaa cgccaggccc
      601 attcggcgca gcggatccgc gtagagcatg gcaccggcca cctggccaac tggcgggccc
      661 cgtcccgcca cctcggccga cgcgaccacc tcgataccat cctgcgcgcc gtcgccggac
      721 tggtctccag ccaggaacaa gccctccgac ccgaccagca ccgccgccca ccgagagctc
      781 tcccggccgg cacagctggg tgaccgaaac ctgccctgaa ccgagcaacg accgccgctg
      841 accgtgcacg aggccgttca agatcgtttt attatgactc cggcgcgcct tcgcgcgctg
      901 ttcggaggtc accggtcgac gccttccggg accatgatca tcggcgtagc gtcgcggacc
      961 cgcacgacct cgttcgatca ggaaggacca ccgttgggtg aggcgcgagc tcgtctggag
     1021 caaaacatcc agcattggaa cgctcacgac tcggcggcct gggtgggcga cttcagcccc
     1081 actgcgcaga tcgtcggccc tggggccagc ggctcaggta tcgagatggc caggacgttc
     1141 tactccatct ggcaggacgc gttcccggac aacgaggttc gcgtgaccga catctttgag
     1201 gacggtgcca cggccattct gcaagccgag tttcggggca cccagacgga gacgatgaac
     1261 gcgcctgagc agtcgatccc ggcgactgga aggcgggtca acatcccctt cgtcctgatc
     1321 aacaggttcg acggtggaaa gaccacagcg tttacgctct atttcgaccg tgccgagttg
     1381 ctggggcagc tcggccttgc gccagctccc agctgagcag gctacgggca ccgcaccaac
     1441 ctccacctcg gcctgtaagc ttcggtcgtt ctggaatgag ttcatacgac ctcgtgcatg
     1501 gttagcggac cgcttgctcc taacaacctc gtgcatggtt agcggcgggc ggtcgttgac
     1561 ctgttcagtg ccagtgcgat cacgcggcgg tgccggccgg tgaagccttc ggtgaacggt
     1621 ggagcggttc gggtcgtggg gctcgttcct ggctggagac cagtccggcg accgcgggga
     1681 gcatcgtctc caggtgctcg cgtcggccga ggtgccggga cagggcgcgc cagttcttca
     1741 ggtggctgat gccgtgctcg acgcggatcc gcttggacgc gtgagccctg cattcggcct
     1801 cgtgtgctgc ggcgagggcg ggtggaaccg gcagctggtt ctttcgccgg gccggccgcg
     1861 gagtgatcac cgcgccggcg gtctgggtgc tcaggccctg atagccgggg tcggcgagca
     1921 gggtgacgcc gggggtgagg gccagcagct cgaccaggcc ggcctggcgg acctgggtca
     1981 ggtcatggac ggcgcccggg agggtctgtc cgcagaacag cagccgccca gcaaggtcgg
     2041 tgatcaccag cgccttgacc gtgttggccc gggccttgcc cgagacgaac cgctggcgac
     2101 cggctttgtg ggctgccggg cggcgcaccg cacctccgtg gcattcagca ggcccagctg
     2161 cctgctcgcc cccaggtggg cgaccacatc ggccagggtc cgcaaccgga gcccgccctc
     2221 cacggtgcac ccgcgttcgg ccagcagcgg gcgcagctct ccgatcgccc gggtgatcgt
     2281 ggagcgggag accccgcacc agcaggccag cacgtcatgg gtgaccccgt gacgtagatg
     2341 caccagggtg gccagcagcc ggtcgaggaa caccagccgg taccgggcac cggctccgac
     2401 cgcccgccgg cgcggccgat cggccagccg cgcgtcctgg cgcgcctgcc agcgcggccc
     2461 cagctcggcc acgagctcag cgagcacttc ccgggacagc ccggtcacca ggcgatcagc
     2521 gaccgcggcg gcacgagcca gcgtcctcga cacaacacga ccttgctccg tccgtcacga
     2581 gcaagcggtc ccgctaacca tgcacgaggt ggtacgagcg ctgcaccgtc ctccagtacg
     2641 agcggctgcg gttcaagtac acgcctgcgc ccaagccgtc gctctggaat gagcggccga
     2701 t
//
LOCUS       DATADN010000476         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1419151, whole genome shotgun sequence.
ACCESSION   DATADN010000476 DATADN010000000
VERSION     DATADN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419151"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1714
                     /locus_tag="VHH53_06205"
     CDS             167..1714
                     /locus_tag="VHH53_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2289778.1"
                     /translation="MRSDPTTCDVLVVGAGPAGLTVATMLARHGVDVLVVERHNGTSP
                     FPKATGVSTRTMELLRSWGLEQRVCAGAMRLRPGLVVSDTLADPTQKTELPFGYPTDE
                     AALAVSPTAPCYCPQDHLEPVLLEYLRDQGGRVRFSTELINFTAGDADVVAELRDRAS
                     GRTERIQARYLIGADGPRSTVRGRLGINVDDLGTIGEFIAVTFRADLTRRLPRPPSVL
                     NAVEITGAAGLFVPTSTDDRWIYGREWHPQRRESIADWTPQRCTELLRAGTGLPDLQP
                     QILTVIPFVMGGHVATAFGAGRTFLVGDAAHRTTPVGGIGMNTAIHGAHNLGWKLAWV
                     LRGWAGQALLDSYEAERRPIGTENVLRSLHPGSQRAGEGLAWDVNVRYTSAVIRAGTG
                     ERAPHVWLRHSGSRVSTLDLFDGRLTVLTSPRGGPWHRAATELAADGLPIVALSADRD
                     LHDEDGAFARHYRLGDAGAALIRPDGYLAWRQLGRGGDARTSLRSAVGFALGRAAGTP
                     ATLRRAG"
BASE COUNT          269 a          657 c          630 g          288 t
ORIGIN      
        1 gccaagaccg gggtgaagga ccgcgccgga ttgcgcgccc tcaggcggcc ggacccgtag
       61 cgccctgcgt aaccgggcca ggcgcaaact ccgtagcgcc gacggatccg cgaccggccc
      121 cgtcgggccg acgctggtgc caagcccacc agcgaaggag cgttccatgc gatccgaccc
      181 caccacctgc gatgtcttgg ttgtcggtgc cgggccggcc ggtctgaccg tggcaaccat
      241 gctcgcccgg cacggcgtgg acgtgctggt cgtcgagcgc cacaacggca cgtcgccgtt
      301 cccgaaggcc accggggtga gcacccgcac gatggagctg ttgcgcagtt ggggcctcga
      361 gcagcgggtg tgcgcgggtg cgatgcgcct gcggccgggc ctggtggtgt ccgacacctt
      421 ggccgatccg acacagaaaa ccgagctgcc gtttggctac ccgaccgacg aggccgctct
      481 ggccgtcagc ccgaccgctc cctgctattg cccacaagac cacctcgagc ctgtgctcct
      541 tgagtacctg cgcgaccagg gcggccgcgt ccgattcagt accgagctga tcaatttcac
      601 ggccggcgac gcggatgtgg tcgctgagct tcgcgaccgc gcctcagggc gcaccgaacg
      661 gatccaggcc cggtatctca tcggcgcgga cggcccgcgc agcacggtgc gcgggcgtct
      721 aggcatcaat gtcgatgacc tcggcacaat cggtgagttc atcgcggtga cttttcgcgc
      781 cgaccttacg cgtcggctgc cgcgcccccc gtcggtactc aacgcggtcg agatcacggg
      841 cgccgcgggg ctgttcgtgc ccaccagcac cgatgaccgg tggatttatg gccgcgagtg
      901 gcaccctcag cgtagggagt cgattgcgga ctggacacct cagaggtgca ccgagctgct
      961 tcgggccggt actggcctgc ccgacctcca accccagatc ctcaccgtga taccgttcgt
     1021 tatgggcggc cacgtcgcca ctgcgtttgg agccgggcgg acatttctcg tcggcgacgc
     1081 cgcgcaccgc accacaccag tcggtggcat cggcatgaac accgcgatcc acggcgcgca
     1141 caacctgggt tggaagcttg cctgggtcct tcgcggttgg gccggacagg cactgctcga
     1201 cagctacgag gccgagcgcc gaccgatcgg aaccgaaaac gtgctgcgct cgctgcaccc
     1261 gggctcccag cgtgcaggcg agggcctggc ctgggacgtc aacgtgcgct acacctcggc
     1321 ggtgatccgg gccgggaccg gcgaacgggc tccgcacgtg tggctgcgac atagcggatc
     1381 ccgggtctcg accctcgact tgttcgacgg ccgtctcacg gtgctcacca gcccgcgggg
     1441 tgggccgtgg caccgggcgg ccactgagct cgccgccgac ggcctgccga tcgtggcact
     1501 gtccgcggac cgcgacctgc atgacgagga cggggcgttc gcccgtcact accgcctagg
     1561 tgacgcgggt gcggccctca tccgccccga cgggtatctc gcatggcgcc agctcgggcg
     1621 gggcggtgac gcgcggacgt cgctgcgctc agctgtcggg ttcgccctgg gccgggccgc
     1681 cgggacgcca gccaccctgc ggcgagccgg atgaactcag ccgacccgct cgtccatgcc
     1741 atccacctgg agcaggagtt tcgctcgccc gtggctgacc gcttccggtg acgtgtcctg
     1801 gtgatcggag ccttcgactg cgcatggtca agcacgtgtt gccc
//
LOCUS       DATADN010000477         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_197135, whole genome shotgun sequence.
ACCESSION   DATADN010000477 DATADN010000000
VERSION     DATADN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197135"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..828
                     /locus_tag="VHH53_06210"
     CDS             223..828
                     /locus_tag="VHH53_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659599.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX2289779.1"
                     /translation="MSELSELRKQATSCTACELYAGATQTVFGAGREGAPIMLAGEQP
                     GDQEDRQGQPFVGPAGKLLDQALQRAGISREDAYVTNVVKHFRFTQRGKRRIHQTPGI
                     EHVRACLPWMRAELQAVDPKILVTLGATAAKSLLGSSFRLTHHRGELLEFDGLPLVAT
                     VHPSAVLRAPDERRDEMLDGLVADLELAGRTAVSFQAADPR"
     gene            complement(800..>1924)
                     /locus_tag="VHH53_06215"
     CDS             complement(800..>1924)
                     /locus_tag="VHH53_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2289780.1"
                     /translation="TAGYDQGGGRTDQAGEPMMTALRTVLTAALTVAAFTLTGCTGNL
                     TSASTPASLIPLPAAVPDLQQAYITVVARVLPSVVQITADGSLGSGIVFDTGGNIVTN
                     AHVVGQATKFDVRLADNPKPLPATLVGSYPPGDLAVIKLDQPPSSLRPAQFGDSAVLQ
                     VGQIVLAMGNPLGLDGSVTNGIISATGRTVTEPPQGAIPGATLADVIQTSAAVNPGNS
                     GGALVDLDGKVVGIPTLGATQPQVDGNGSSPASGIGFAIPSNVVTDIAGQLIANDGRV
                     VNSRRAALGVQIVTVVNSTGQPTGMGIVSVTPGGPAATAGLQADEVIASVNDTPVRRA
                     AELAQVLAELDPGQTVPVTVTTPQGVSRTVDVTLGQLPGS"
BASE COUNT          311 a          612 c          691 g          310 t
ORIGIN      
        1 gccagcgcat ccccgtgcaa gccggccggg cacgccagct cgactccggc cagcaagctg
       61 agaccggcaa taccaccgag gggaccagcg acgccagcgc aagtgccggc gaaaagtagt
      121 aggtgacacg ccgcagcctg agcggccaac ccgctcaggc tgcatgtcgc cggcgtgtcg
      181 gcggagggtt agacattccg gcctgcgggg tacgccggtg gcatgtccga gctgagtgaa
      241 ctgcgcaagc aggccacgag ttgcacagcg tgcgagttgt acgccggcgc gactcaaacg
      301 gttttcggcg ccgggcgcga gggtgcaccg atcatgctgg ccggtgagca gcccggggat
      361 caggaggatc gccaggggca gccgttcgtg gggcccgccg ggaagctgct ggatcaggcg
      421 ctgcagcggg ccggcatctc acgcgaagat gcctacgtca ccaatgtcgt taagcatttc
      481 cgcttcacgc aacggggcaa gcgacggatc caccagacac ccggcatcga gcatgttcgg
      541 gcctgcctgc cctggatgcg tgccgagctg caggcggtcg acccgaaaat actggtgacc
      601 ctcggggcga cggccgccaa gtcgctgctc ggctcgtcgt ttcggctcac ccaccatcgc
      661 ggtgaactgc tggagttcga cgggctgccg ctggtggcga cggtccaccc gtcagcggta
      721 ctgcgggctc ccgacgagcg ccgtgacgaa atgctcgacg ggctggtcgc tgacctggag
      781 ttggccgggc gtaccgcggt cagcttccag gcagctgacc caaggtgacg tcaacggtgc
      841 ggctgacacc ctgcggggtg gtcacggtga ccgggacagt ctgtcctggg tctagctcgg
      901 ccagtacctg ggcgagttct gcggcacggc ggactggggt gtcgttcacc gaggcgatca
      961 cctcgtcggc ttgcagcccg gccgttgcgg ccggtccgcc gggagtcacg ctgacaatcc
     1021 ccatgccggt cggctggccg gtcgagttca ccacggtgac tatttgaacg ccgagcgcgg
     1081 cgcggcgaga gttgaccacg cgaccgtcat tggcgatgag ctgcccagcg atgtcagtga
     1141 cgacgttgct ggggatcgcg aaaccgatgc ccgatgcggg tgagctgcca ttgccatcaa
     1201 cctgcggttg ggtcgcaccc agggtgggta tgccgacgac cttgccgtcc aggtcgacca
     1261 gcgcgccgcc gctgttgccc gggttgaccg cggcactggt ctggatgacg tcggccagcg
     1321 tcgcgcccgg tatggcgccc tggggaggtt cggtgacggt gcggccggtg gccgagataa
     1381 tgccgttggt gacgctgccg tccagcccga gcgggttgcc catggccagc acgatctggc
     1441 cgacctgcag gacggcggag tcgccgaatt gcgccgggcg cagcgatgac ggtggctggt
     1501 caagcttgat gacagcgagg tcgccgggcg ggtaggaacc caccagtgtg gccgggagcg
     1561 gtttcggatt atcggccagg cgcacgtcga acttcgtcgc ctgcccgacc acgtgggcgt
     1621 tggtgacgat gttgccgccg gtgtcgaaga ctattccgga gcccagcgaa ccgtcggcgg
     1681 taatctgcac gaccgagggc agcaccctgg ccaccacggt gatgtaggcc tgctgcaggt
     1741 cgggcacagc cgccggcagc ggaatcaggc tggccggggt gctggccgac gtgagattgc
     1801 cggtgcaccc agtgagagtg aacgcggcaa cggtcaacgc ggccgtcagc acggtgcgta
     1861 acgctgtcat catcggctcc ccagcttgat cggtgcgccc accaccttga tcatatcctg
     1921 ccgt
//
LOCUS       DATADN010000478         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_41136, whole genome shotgun sequence.
ACCESSION   DATADN010000478 DATADN010000000
VERSION     DATADN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41136"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1315)
                     /locus_tag="VHH53_06220"
     CDS             complement(92..1315)
                     /locus_tag="VHH53_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289781.1"
                     /translation="MLLSVNTIVHLELKVELAPVTINGLAEWVFGKREELTQMLLAPL
                     VRAAQEQWLSEVERSAAELICTGCGVVHQGAGGWVRRGSRVRSVKTSSGEVDLALLQV
                     TCRECGKTRAPCVEVLGLEPRRQATPEFTRKLVERVYETSYHRSVRTARECMGTSVSA
                     STLHRFVQERAARVHLTPSPQSETILADGTPVRAGERQRDGKRVEHEELRLACQLLGR
                     TEEGGRPKAHLRLIGIGVGTKTWPAVLPGDKRTKLVVTDGEPALLSHVRTRYPQARHQ
                     LCEWHLPHTLDWPLRKDGVGVKERRRLQDELKSILWGNRLPSKSRQLYSAFAERLSFS
                     PSAQYQLRQAAPYVLYDEPSAERTTSLMERQMREVDRRVQIGVRWSIRGVRNLMLLSL
                     ARRHNADDYDRAWSN"
BASE COUNT          259 a          629 c          506 g          312 t
ORIGIN      
        1 cggaccccca cccccggccc ctctcccgca actgcggtag tgttcgatcg cgttgacatc
       61 ggcgttgagg caccgatgcg cgcgcgatcg atcagttgct ccacgcgcgg tcgtagtcat
      121 ccgcgttgtg cctacgggca aggctcagga gcatcaggtt gcgcacgccg cggatcgacc
      181 atcgaactcc gatctgcacc cgccgatcca cctcccgcat ctgccgctcc atcaagctcg
      241 ttgtccgctc cgccgaaggt tcgtcgtaca ggacgtatgg cgcagcctgc cgcaactggt
      301 actgcgcgct cgggctgaac gacagccgct cggcgaacgc gctgtacaac tgccggctct
      361 tgctcgggag ccgattcccc cacaggatcg acttcagctc gtcctgcagc cgcctgcgct
      421 ccttcacgcc caccccgtcc ttgcgcagcg gccagtcgag tgtgtgaggc aggtgccact
      481 cacatagctg gtgccgcgct tgcgggtacc gcgtgcgcac gtgggacagg agcgcgggct
      541 cgccatcggt gaccaccagc ttggtgcgct tgtcgcctgg cagcacggcc ggccacgtct
      601 ttgtccccac accgatcccg atcaaccgca gatgcgcctt cggccggcct ccctcctcgg
      661 tccggccgag aagctggcag gcaagacgca gctcctcgtg ctccacccgc ttcccatccc
      721 gctgccgctc tcccgctcgc accggggtgc cgtccgcaag gatcgtctcg ctctgcgggc
      781 tgggcgtcag gtgcacccgc gccgcgcgct cctgcacgaa gcggtgcagc gttgaggcgg
      841 agacgcttgt gcccatgcac tcgcgcgccg tccgcacgct cctgtggtag ctcgtctcat
      901 agactcgctc cacaagcttg cgcgtgaact ccggcgtggc ctgccgccgc ggctccagtc
      961 ccagcacctc cacgcacggc gcgcgtgtct tgccgcactc gcggcacgtc acctggagca
     1021 atgccaggtc aacctctccg ctgctcgtct tcacgctccg cacccggctg ccgcggcgca
     1081 cccacccgcc agcgccctgg tgtaccaccc cgcagccggt gcagatcaac tctgcggcac
     1141 tcctctccac ctcactcagc cactgctcct gtgccgcccg gaccagcggc gcgagcagca
     1201 tctgcgtgag ctcttcgcgt ttgccgaaca cccactccgc aagaccgttg atcgtaacgg
     1261 gcgcgagctc taccttcagt tccaggtgga caatggtgtt caccgacagg agcatggggc
     1321 acctcccttt cttgcgttgg ggtgtggtgc ctcatgcttc tgcttttcca cctgatgtca
     1381 acgatatcga acactaccca aactgcgtgg gggaagggag aactgctcgt cactcggcgg
     1441 ccgcggtggt gtccgcgaag gcggacagcg cgtagtcgtt gcggcgatgc ggcgatttca
     1501 atcgcttgtc gcggggccgg ccttcaccct ccggggtccg ggcgcccctg cgggctgaca
     1561 gccgtgactc tcttgcatta tcatgagagc gcagcgaaac aggcagcgaa ccagttccgg
     1621 tgacgcatga caaacgcatt cctggaagat cgcatcaccg tgtctcaggt ggagatcgac
     1681 gagttctgcc ggcggaacca catccg
//
LOCUS       DATADN010000479         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_512937, whole genome shotgun sequence.
ACCESSION   DATADN010000479 DATADN010000000
VERSION     DATADN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512937"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..904
                     /locus_tag="VHH53_06225"
                     /pseudo
     CDS             68..904
                     /locus_tag="VHH53_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
     gene            1079..1852
                     /locus_tag="VHH53_06230"
     CDS             1079..1852
                     /locus_tag="VHH53_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2289782.1"
                     /translation="MSTTRPLPRFVRYRPPQPRSGPAPRGAVLLWPDTFTRSFDPQVA
                     YAAARVLTAAGFAVRLPRGTLCCGLTWLVTGQFSTARVVLRRTLRLLRSEIRAGVPVV
                     GLEPSCVATLRCDLTELLPDDPAAAVLARQARTLAEVLHERAPDWRPPERDRDAVQQV
                     HCHQHAALGHDADDALLRAAGVRVQRVSGCCGLAGSFGYQRGHEEMSSALAERSLAPA
                     VRARPDALVLADGFSCRLQTSQTTGRRALHLAELLDGSC"
BASE COUNT          243 a          648 c          730 g          310 t
ORIGIN      
        1 agcgcagctt cctgagggcg cggcctggct tctcgtcgag ttcggcgcgg acgactcaag
       61 ggcagcggtg gcggcggcgc aggccgcggc ggccgacgtc acccggccgc atctggtggt
      121 ggccgatccg gcgcaccggg cggccctgtg gcgaatccgc gaggcgggcg cgggtctggt
      181 gacccggttg gccgacggtt cggaggcatg gccaggctgg gaggacgcca cggtgccccc
      241 cgaccggctc ggcgactacc tacgtggctt cgctgacctg ctcaccgagc atcgccgtgc
      301 ggggtcgatc tacgggcatt tcggcgaggg ctgcgtacac gtgcggaccg atgccgacct
      361 gctcacctag gccggccgtc gggacttccg gcaattcctg gagcaagccg cagatctggt
      421 ggtgacccac cgcggttcgc tgtccgggga gcatggggac gggcgggccc gcagcgagct
      481 gctggaccgg atgtacccgc gtgagatgct cgacctgttc gcgcggttca aggagatctt
      541 cgatccggac cggctgctca accctggagt gctggtagat ccccagccgc tggacgatga
      601 cctgcgacct ggccgggagc gcgacctgcc ggtgactctg gcgttgacgc ccgacggggg
      661 cagcctggcc gcggccaccc ggcgctgcgt cggcgtcggt gcctgccggc agatggaagg
      721 tcccggcgtg atgtgtccca gcttccaggt cacccgggac gagaagcact ccacccgcgg
      781 ccgggcgcac ctgctggccg agatgctggc cggtgacctg gtgaccggcg gctggcagtc
      841 ctcggagctg cacgaggcgt tggacctgtg cctggcctgc aaggcgtgcg cctcggactg
      901 cccggtcaac gtggacatgg ccagctacaa ggccgagttt ctccaccagc gttaccttca
      961 ccggttgcgg ccgcggtggc actacgcgtt gggcgggttg ccgctggcgg cctgggcggc
     1021 cgggttcgcg ccggtggtgg ccaaccgggc tgcgccgtgg ctggccaagc tgggtgggat
     1081 gtccacgacc cgaccgctgc cccgattcgt ccgttaccgg ccgccgcaac cgcggtccgg
     1141 gcccgcgcca cgcggtgcgg tgttgctgtg gccggacacc ttcaccagat cgtttgaccc
     1201 ccaggtggcg tacgccgcgg cgcgagtgct caccgcggca ggcttcgccg tccggctgcc
     1261 ccggggcacc ctgtgttgcg ggctgacgtg gctggtcacc gggcagttca gtaccgcccg
     1321 ggtggtgctg cggcggacac tgcgactgct gcggtcggag atccgtgccg gggtgccggt
     1381 ggtcggtctt gagcccagct gcgttgccac gctgcgctgt gacctgaccg agctgttgcc
     1441 ggacgacccg gccgcggccg tgctcgccag gcaggcgcgc acgctcgccg aggtgctgca
     1501 cgagcgtgcg ccggactggc ggcctcctga acgtgaccgg gatgcggtgc aacaagtgca
     1561 ctgccaccag catgcagcgc tgggtcacga cgctgacgat gcgctgctgc gcgcggccgg
     1621 ggtccgtgtc cagcgggtat ccgggtgctg cggattggcc ggcagtttcg gttaccagcg
     1681 cggtcacgag gagatgtcca gcgcgctggc cgaacgttcg ctggctccgg cggtccgagc
     1741 gcgccccgac gcgctggtgc tggccgacgg gttcagctgc cgattgcaga caagccagac
     1801 gacaggccgg cgtgcgctgc accttgccga gttgctcgac ggaagttgct gaccgagcgc
     1861 tgagcggtga gattcggatc ggctgatgac ggttcacccg cggctatcac ccagagggag
     1921 gctgcgctgc c
//
LOCUS       DATADN010000480         1880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1419576, whole genome shotgun sequence.
ACCESSION   DATADN010000480 DATADN010000000
VERSION     DATADN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419576"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VHH53_06235"
     CDS             complement(<1..849)
                     /locus_tag="VHH53_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX2289783.1"
                     /translation="MDGTIVYVLVIVLIALTFDFINGFHDSANSIATVVGTRVLKPLP
                     AVIWAAFFNFVALFTVGTAVAKAIGKGMIDLDVVTPTVILGGLSGAIIWNLITWFYGL
                     PSSSSHALIGGYAGSAVAAAGMGAIEWGTKWMQTLAFIVLSPLIGMFLGFTLMVLVSW
                     MFHRVPRGPADRFFRVAQLGSSALFSLSHGANDAQKTMGIIVSLLVSVNHVFATQTGW
                     LHNLYVPNADHIPFWVELCAYSALSLGTLFGGWRIVHTMGTRITRLRPVGGFCAETGG
                     AAAIFFA"
     gene            complement(852..1472)
                     /locus_tag="VHH53_06240"
     CDS             complement(852..1472)
                     /locus_tag="VHH53_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX2289784.1"
                     /translation="MRLLPRDEKFFDLFSAVATFTVEAAKLLQELLRSDAQRRTAIVD
                     QIKRLEHQADQVTHEVVTRLDRVFITPLDREDIHLLASRLDDVIDLIDGTARRLLMFR
                     AGRSPEGAVLIADVIAKAVTELQVAVRALEKNKGGTVLAACVQVKRMEEEGDSLYHEW
                     LGRLFEGEPAPLEVIKWKEIYDNLEKTLDFIEDAANVLESISIKHA"
     gene            1573..>1880
                     /locus_tag="VHH53_06245"
     CDS             1573..>1880
                     /locus_tag="VHH53_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2289785.1"
                     /translation="MAPPTDARARLAAVARTALLDTPPEEAFDRLTRMAARLLGAPIS
                     LITLVTDDRQFFKSAMGLPEPWASRRTAPLSQSFCRQVAVSGEPLVVEDTRRHPLLRT
                     "
BASE COUNT          312 a          674 c          620 g          274 t
ORIGIN      
        1 ggcgaagaag atcgccgccg cgcccccggt ctccgcgcag aacccgccca ccggccgcag
       61 ccgcgtgatg cgcgtgccca tggtgtgcac gatgcgccac cccccgaaca gcgtccccag
      121 cgacagcgcc gagtaggcgc acagctccac ccagaagggg atgtggtccg cgttggggac
      181 gtacaggttg tgcagccacc ccgtctgggt cgcgaagacg tggttcaccg agaccagcag
      241 gctcacgatg atccccatcg tcttctgcgc gtcgttggcc ccgtgcgaca gcgagaagag
      301 cgcggagctc cccagctgcg ccacccggaa gaagcggtcc gccgggccgc gggggacgcg
      361 gtggaacatc cacgacacca gcaccatcag cgtgaacccc aggaacatgc cgatcagcgg
      421 cgacaggacg atgaacgcca gcgtctgcat ccacttggtg ccccactcga tggcgcccat
      481 tcccgccgcg gccaccgcgg agcccgcgta cccgccgatc agcgcgtgcg acgagctgct
      541 gggcaggccg tagaaccagg tgatcaggtt ccagatgatg gcgccggaaa gcccgcccag
      601 gatcaccgtg ggggtgacga cgtccaggtc gatcatcccc ttgccgatgg ccttggccac
      661 cgccgtgccc acggtgaaca gcgccacgaa gttgaagaac gccgcccaga tgaccgccgg
      721 cagcggcttg agcacgcggg tgcccaccac cgtggcgatg gagttggcgc tgtcgtggaa
      781 gccgttgatg aagtcgaagg tgagcgcgat gaggacgatc accaggacgt agacgatggt
      841 gccgtccatg ctcaggcgtg cttgatggag atggactcca ggacgttggc cgcgtcctcg
      901 atgaagtcga gcgtcttctc caggttgtcg tagatctcct tccacttgat cacctcgagc
      961 ggcgcgggct caccctcgaa gagccggccc agccactcgt ggtagaggga gtcgccctcc
     1021 tcctccatcc gcttcacctg cacgcacgcg gccagcacgg tgccgccctt gttcttctcc
     1081 agcgcccgca cggcgacctg cagctccgtc acggccttcg cgatcacgtc ggcgatgagc
     1141 accgccccct ccggcgaccg gccggcgcgg aacatgagca gccggcgggc cgtgccgtcg
     1201 atgaggtcga tcacgtcgtc gagccgggag gccaggaggt ggatgtcctc ccggtcgagc
     1261 ggggtgatga acacccggtc gagccgggtc accacctcgt gcgtcacctg atccgcctga
     1321 tgctccagcc gcttgatctg gtccacgatg gcggtgcgcc gctgggcgtc ggaccggagc
     1381 agctcctgca gcagcttcgc cgcctccacg gtgaaggtgg cgacggcgct gaacagatcg
     1441 aagaacttct cgtcgcgagg gaggagccgc atgtgtcaca tccaggtcac ggaagcgagc
     1501 ggtcgggaag atagcaccgg cccagccggt gagctatcag ccccgcggct ccggaggtaa
     1561 cttcgtggcg gtatggcccc ccctaccgac gcccgcgcgc ggctcgccgc cgtggccagg
     1621 accgcgctcc tggacacccc gcccgaggag gccttcgacc ggctcacccg gatggcggcg
     1681 cggctcctcg gcgccccgat ctcgctcatc accctggtca ccgacgaccg ccaattcttc
     1741 aagagcgcca tgggcttacc cgagccctgg gccagccgcc ggaccgcccc gctgagccag
     1801 tcgttctgcc gccaggtggc ggtctcgggc gagccgctgg tcgtggagga tacccggcgc
     1861 catccgctgc tccgcaccga
//
LOCUS       DATADN010000481         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_660540, whole genome shotgun sequence.
ACCESSION   DATADN010000481 DATADN010000000
VERSION     DATADN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660540"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VHH53_06250"
     CDS             complement(<1..1014)
                     /locus_tag="VHH53_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator"
                     /protein_id="HEX2289786.1"
                     /translation="MATADPTAMDAGRMTRAQRAALPLSADVAQALAEQHGVCIRPLA
                     MRRIDTATGRVDIVPVACGSTRDDQCRPCADKARRLRMAQCRQGWHLEDEPVTSRAAP
                     TEDHTALMATRADLLAVYAECQAIGDEITGEQIADSIAEVDTELRATGVRGRLAPLDP
                     VPKPVKRSTRRRQDAPNLPRRPVQRRTVGRVFAGRYRPSTFLTLTLDSYGRVDDSGAA
                     VDPDRYDYRRAARDAIHFPALMDRFWQNTRRCVGWDVQYFGTVEPQKRGAPHFHAAIR
                     GAIPRAELRAITAATYHQVWWPAHDQLVYTDQRMPVWDIRAKAFTDPDTGTPLPTWDQ
                     ACEE"
BASE COUNT          252 a          528 c          577 g          311 t
ORIGIN      
        1 ctcctcgcac gcctgatccc aggtgggcag cggtgtgccg gtgtcggggt cggtgaaggc
       61 ctttgctctg atgtcccaga cgggcatccg ttggtcggtg tagacgagtt ggtcatgagc
      121 aggccaccac acttggtgat acgtcgctgc cgtgatcgcc cgtaactcgg cgcgagggat
      181 ggctcctcgg atcgcggcgt ggaagtgtgg ggcacctcgc ttttggggtt cgacggtgcc
      241 gaagtactgc acgtcccacc cgacgcagcg gcgggtgttt tgccagaacc ggtccatcaa
      301 cgccgggaaa tgaatcgcgt cccgggcggc ccgccggtag tcataccggt cggggtccac
      361 cgccgcgccg ctgtcatcga cccggccgta ggagtccagg gtgagggtca gaaatgtcga
      421 gggccggtag cgcccggcga agacccggcc gaccgtgcgc cgctgtaccg gccgacgggg
      481 cagatttggt gcgtcttggc ggcgtcgggt ggagcgcttg actggcttgg gtacggggtc
      541 aagcggggcg agccggccac ggactccggt ggcgcgcagt tcagtgtcga cttcggcgat
      601 cgagtcggcg atttgctcac cggtgatctc atcgccgatg gcctggcatt cggcgtagac
      661 cgccaggaga tcggcccggg tggccatcag cgccgtgtgg tcttcagtcg gcgcagcccg
      721 gctggtgacc ggttcgtcct ccagatgcca accctggcgg cactgggcca tccgcaaccg
      781 acgggccttg tccgcacacg gccgacactg gtcgtcccgg gtggacccgc acgcaacggg
      841 cacaatgtcg acacggccgg tggcggtgtc gatccggcgc atcgccagcg gccggatgca
      901 cacgccgtgc tgctcggcca aggcctgggc cacatcagcg ctgagcggta gcgcggcccg
      961 ctgtgcccgc gtcatccgcc cggcatccat agccgtgggg tcggcggtgg ccatcaggcg
     1021 cccacctccc cctcggtgcc ggcacgccgc agcgcggcga gtgggatagt ggtgacatca
     1081 ggtgccagat cagcgctcag accggaagag atggcctgcg ggccgccgta ggctaggact
     1141 gcgggcgagg gtgcccgtcc gtggccagat ccgacgctgt tgtctgttgc ggtggtcgtg
     1201 gagatcgggt gggggcttgt gcctgccctg gtcgttgtgc gtatcctcac tggtattcac
     1261 tcctgactgg gtggatggcg cagaagcggg acgggttggt agccaggaat ccgcttctgc
     1321 gccggggtct cgttctcaga aaatgggcat aggtgtgctc gccacgtgac ctagtgcccg
     1381 tgatctttcg aattacgtca gtggtctagg gttgttcgcc tgttgtttct gtagcatcac
     1441 ctcccccggt ttggtcttgt cggacagcga cacactctca cccagtgcgc caatcgccaa
     1501 caaagcaagc gcatatcccc accggcgctg accacgcata tgcgtcccac gcataaggac
     1561 agctcaccgg cacgcactgc gatgccctga catatgccgc gcgcatatgc cgttcgtttg
     1621 gagagctgcg atgaccgctt agcacccatc tggtgacccg cataccca
//
LOCUS       DATADN010000482         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_513262, whole genome shotgun sequence.
ACCESSION   DATADN010000482 DATADN010000000
VERSION     DATADN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513262"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VHH53_06255"
     CDS             <1..324
                     /locus_tag="VHH53_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289787.1"
                     /translation="RDFDDAVTARLHGFRDADDYYATSSCARFLHGIRVPTLLVHALD
                     DPFVDERAIPHDVIAANPALHTAFTPHGGHVGFIAGPPWKPRFWAEREAARFLAAHAE
                     SNLRK"
     gene            416..1219
                     /locus_tag="VHH53_06260"
     CDS             416..1219
                     /locus_tag="VHH53_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2289788.1"
                     /translation="MAERFANRLVLNVPTRGNPKVERLIERINADDELYALWTAANVN
                     AVERLGMTDHGPVHVKIVMNIAIKILRVLTEHGVQPGVAANYRMGVDDAEVVVVLASL
                     LHDVGMSIHRKDHEEFSLFVAQPKVRELLAGIYDPGQATILRSEVLHAIIGHRAGGKP
                     MTIVGGVVRVADALDMAKGRSRISMGIEGTMSIHSISAAAIEAVHIETGETKPVRIRV
                     EMSNSAGIFQLDQLFREKLLGSGLEPYVEVEALIEGEAEKRLVQTAYRL"
BASE COUNT          286 a          557 c          600 g          235 t
ORIGIN      
        1 cgcgacttcg acgacgcggt gacggcgcgg ctgcacggct ttcgcgacgc ggacgactac
       61 tacgccacca gcagctgcgc gcgcttcctg cacggcatcc gcgtccccac gctgctggtg
      121 cacgcgctgg acgatccgtt cgtggacgag cgcgccatcc cgcacgacgt gatcgccgcc
      181 aaccccgcgc tgcacacggc cttcaccccg cacggcgggc acgtggggtt cattgccggg
      241 ccgccgtgga agccgcgctt ctgggcggag cgcgaagcgg cgcgattcct ggccgcgcac
      301 gcggaatcaa acctgcgtaa gtagcaggag cgaagcagat ttacacgaac ctggcagata
      361 gaagatgacg gacgtactgg aagcggcgga cgtacggtcc acgctggggt gctcggtggc
      421 ggagcggttc gccaaccggc tggtgctcaa cgtgcccacc cgcggcaacc ccaaggtgga
      481 gcggctcatc gagcgcatca acgccgacga cgagctgtac gcgctctgga cggcggccaa
      541 cgtgaacgcc gtggagcggc tggggatgac cgaccacggg cccgtgcacg tgaagatcgt
      601 gatgaacatc gccatcaaga tcctgcgcgt gctcacggag cacggcgtgc agccgggggt
      661 ggccgccaac taccggatgg gggtggacga cgctgaggtg gtggtggtgc tggcttcgct
      721 gctgcacgac gtgggaatgt ccatccaccg caaggaccac gaggagttct cgctcttcgt
      781 ggcgcagccc aaggtgcgcg agctgctggc cggcatctac gaccccggcc aggccaccat
      841 cctgcgctcc gaggtgctgc acgccatcat cggccaccgc gcgggcggca agccgatgac
      901 catcgtgggg ggggtggtgc gggtggcgga cgcgctggac atggccaagg gccgctcgcg
      961 catcagcatg gggatcgagg ggaccatgag catccactcc atctccgcgg cggccatcga
     1021 ggccgtgcac atcgagaccg gcgagaccaa gcccgtgcgc atccgcgtgg agatgagcaa
     1081 ctccgccggc atcttccagc tggaccagct cttccgcgag aagctgctcg gcagcggcct
     1141 ggagccgtac gtggaggtgg aggcgctcat cgagggcgag gccgagaagc gcctggtgca
     1201 gacggcgtac aggctgtaac gcggcggccg caggaaggcc gggttcactg cgtcggcgtg
     1261 cagcaccgac atcgcgcctg agcggctgaa gccgccgcgc cgcatccagc gtgcggtcgg
     1321 tgccggggct ggacgcggaa gtggcgagga caagcgtggc gtcggaatcg cgcacgttcc
     1381 actccgtgcg ctcggcggga tcggcggagg gcgtttcgcg cagcgggtaa cgtggatcga
     1441 ctgggccatc ctccgcccag cgccccgccg ggcaccagcc gccgcacggc atcccccgct
     1501 ccagcgccac gtccagcgcc gcccggtcca cccccgtctg cccgccggaa acgagggtga
     1561 gagcgaggat gaatcagagt gcattcagct gcgaatcggg aagttgtaac gcaggctttc
     1621 gcgtggcccg cctccagagg cgaataatgc cgattgcgat cctgattcgg taacgtgc
//
LOCUS       DATADN010000483         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_367145, whole genome shotgun sequence.
ACCESSION   DATADN010000483 DATADN010000000
VERSION     DATADN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367145"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..>1589
                     /locus_tag="VHH53_06265"
     CDS             117..>1589
                     /locus_tag="VHH53_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HEX2289789.1"
                     /translation="MPNRITTDTGCRRMGWTIAALALLTGVLGIFAVLAPVIADDPVV
                     SWPRAGQQPTSTVLPLAPYRPLQLTATVPCAALQTLNARPGGGEALRTLPADVGTAPG
                     EGLVVAASQGIVTVTASGAELTREVLPAGNCSYRVLADAGGIRVARDGAGISTRADLL
                     PPQVAQLQTDAVAHTSGLSVTLHTDARYQSQPTPLKTVLLVAHGLALAVLLVLAWRWW
                     RGEGPGLVHPRPSWADAVVVGISLIWVLLAPVNIDDSWYLLMARNAMESGYIGNVIYQ
                     FNVSENPFSASQYALQAWGALGGNWSLAWMRLLPLGYGLVSYMLLRVLVATVLDRAAR
                     RPLVPWAVAAAHLLWWLPYGITLRPEPLIVLGASLVLVLAELARRCRSVGVLAVATAV
                     AAVTVTASPTGLVAVAPLVLCLPWLWQWWRASALAGRVATVLLAGAAASIVVPIGFAD
                     ASLGDVLESIAVHRWYYRQNAWYDEYLHYANLLAPRSSGTS"
BASE COUNT          199 a          572 c          556 g          262 t
ORIGIN      
        1 tgcgatcatc aacccggacc aacgacccct gcccatcccc tgcgccgctg actcgcctcg
       61 ggttaacccc accacccagc gccaaccacg cgcgcccggc cccatagcct gccgcggtgc
      121 cgaaccgcat taccaccgac accggctgtc gccggatggg gtggacgatc gcggcgttgg
      181 cgctgctcac cggagtgctc ggaattttcg cggtactcgc tccggtcatc gccgacgatc
      241 cggtggtgag ctggccccgc gccgggcagc agcccacctc cacggtgctc ccgctggcgc
      301 cctaccgccc gctgcagctc accgccaccg tcccgtgcgc ggcgctgcag acgttgaacg
      361 ctcgcccagg tggcggcgag gcactgcgca cgctgcccgc cgacgtgggc accgcgccgg
      421 gtgaaggcct ggtggtagcc gcatcccagg gcatcgtgac agtgaccgcg tccggtgcgg
      481 agctcacgcg cgaggtactg cctgccggga actgctccta ccgggtcctc gcggatgccg
      541 gcgggatacg ggtggcccgc gacggcgccg gcatcagcac tcgggcagac ctgctgcctc
      601 cacaggtcgc gcagctgcag accgacgcgg tagcgcacac gagtgggctg tcggtgacgc
      661 tgcacaccga cgcgcggtac cagtcgcagc ccacaccgct caagacggtg ttgctggttg
      721 cgcatggtct cgcactcgcg gtgctgctcg tgctggcctg gcggtggtgg cggggcgagg
      781 ggcccggcct ggtccacccg cgcccttcgt gggccgatgc ggtcgtggtc gggatctcgc
      841 tgatctgggt gctgctggcc ccggtcaaca tcgacgactc gtggtacctg ctgatggccc
      901 gcaacgcgat ggaatcgggt tacatcggca acgtcatcta ccagttcaac gtctccgaga
      961 acccgttctc cgccagccag tacgcgctgc aagcctgggg agccctcgga ggcaactgga
     1021 gcctggcctg gatgcggctg ctgccgctgg gctatggcct ggtcagctac atgctgctgc
     1081 gggtcctggt cgcgacggtg ctggaccggg cggcgcggcg accgctggtg ccgtgggcgg
     1141 tggcagccgc gcacctgctg tggtggttgc cctacgggat cacgctgcgg cccgaacctc
     1201 tgatcgtgct cggcgcgtca ctggtgctgg tactggcgga gttggcccgc cggtgccgct
     1261 cggtcggggt gctcgcggta gccaccgccg tcgccgcggt caccgtcacg gcctctccca
     1321 ccggcctggt cgcggtcgct ccgctggtgt tgtgcctgcc gtggttgtgg cagtggtggc
     1381 gagcctcggc cctggccggg cgggtagcga cggtgctgct ggccggggcg gccgcctcga
     1441 tcgtggtccc gatcggattc gccgacgcca gcctcggcga cgtgctggaa tccatcgcgg
     1501 tgcaccgctg gtactaccgc cagaacgcct ggtacgacga gtacctgcac tacgcgaacc
     1561 tgctggcacc ccgctcgtcg ggtaccagc
//
LOCUS       DATADN010000484         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_42233, whole genome shotgun sequence.
ACCESSION   DATADN010000484 DATADN010000000
VERSION     DATADN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42233"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VHH53_06270"
     CDS             <1..366
                     /locus_tag="VHH53_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX2289790.1"
                     /translation="QRALVRMLREAGAIEVHVRIASPPVRWPCFYGIDFASHAELIAD
                     CADGGDVDAIRRGIGADSLGYVSLENLIAASEQPASRLCTACFDGRYPIPLPPSESIG
                     KHLLEDVGQESHQPALRIG"
     gene            468..1565
                     /gene="purM"
                     /locus_tag="VHH53_06275"
     CDS             468..1565
                     /gene="purM"
                     /locus_tag="VHH53_06275"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX2289791.1"
                     /translation="MTETDSPHTTYTAAGVDITAGERAVAQLRPHAERASRPEVLGGI
                     GGFAGLFKPKLDRWREPVLASSTDGVGTKIVVAQAMDCHHTIGLDLVAMVVDDLVVCG
                     AEPLFLQDYIAVGKVVPERISDLVSGIAEGCIQAGCALLGGETAEHPGMMADGDYDLS
                     ATGVGVVEADAILGPDRVRPGDVVVAMGSSGLHSNGYSLARHVLLDIARMPLDGHVEE
                     FGRTLGEQLLEPTRIYAKDCLALVAETEVRTFAHITGGGLAANLTRVMPRGLVAVLDR
                     GSWTPAAEFALIARLGRVQRAEMEHTFNMGVGMVAVVDAAEVDRALAVLTARHVPAWV
                     LGEVRRSRDVGVDATAKDAVRAVLHGDHPRF"
     gene            complement(1600..1797)
                     /locus_tag="VHH53_06280"
     CDS             complement(1600..1797)
                     /locus_tag="VHH53_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HEX2289792.1"
                     /translation="MGRGRAKAKQTKVARELKYSSPTTDLEALQRELSGGNGGRHASE
                     DRGSDDRTEDLYEDGYDQHRR"
BASE COUNT          334 a          735 c          792 g          391 t
ORIGIN      
        1 cagcgcgctc tggtgcggat gttgcgggag gccggcgcga tcgaggtaca cgttcgaatt
       61 gccagcccac cggtgcgctg gccgtgcttc tacggcatcg acttcgcctc ccatgccgaa
      121 ctcatcgccg actgcgccga cggcggcgat gtcgacgcga tacggcgcgg gatcggagct
      181 gacagtctcg gctatgtcag cctcgagaac ctcattgccg catccgagca gccggcgtca
      241 cggctgtgca cagcgtgttt cgacggtcgc tacccgatcc cgctgccgcc cagcgagtcc
      301 atcggtaagc acctgctcga ggacgtcggg caggaatcac accagccggc gttgcggatc
      361 gggtgagcag cgccgggacg ccgcgcccat acctgagcga gtagcgtgac ctactgctcg
      421 taaggcatct tcgcgccacc agacccgtgt cctggagttc gcatgccatg accgagaccg
      481 atagcccgca caccacctac accgcagccg gggtcgacat caccgccggg gaacgggcgg
      541 tcgcccagct gcgcccgcac gccgagcggg ccagccgccc ggaggtactg ggcggcatcg
      601 gcggcttcgc tggcctgttc aagcccaagc tcgaccgctg gcgtgaaccc gtactggcga
      661 gctccactga cggtgtaggc accaagatcg tggtggccca ggcgatggac tgccaccaca
      721 ccattgggct cgacctggtg gcgatggtcg tcgacgactt ggtggtctgc ggtgccgagc
      781 cgttgttcct gcaggactat atcgccgtcg gcaaggtcgt gccggagcgg atctcagacc
      841 tggtcagcgg cattgccgaa ggctgtattc aggctggctg tgctctcctg ggcggggaga
      901 ccgccgagca cccagggatg atggccgacg gcgactacga cctgtccgcg accggggtcg
      961 gtgtggtaga ggccgacgcc atcctggggc ccgatcgcgt ccggcccggt gacgttgtgg
     1021 tggcgatggg ctcgtcgggg ctgcactcca atggttactc gctggcccgg catgtgctcc
     1081 tggacatcgc ccggatgccc ctggacgggc atgtggagga gttcggccgc accctgggag
     1141 agcaactgct tgaacccacc cgcatctacg ccaaggactg cctggcgctg gtcgcggaga
     1201 ccgaggtgcg gaccttcgcc cacatcaccg gcggtggcct ggcggcgaac ctgactcggg
     1261 taatgccccg ggggctggtc gctgtgctgg accgcggcag ctggacgccg gccgccgagt
     1321 tcgcgctgat cgcccggctg ggtcgggtac agcgcgcaga gatggagcac accttcaaca
     1381 tgggcgtcgg gatggtcgct gttgtcgacg cggctgaagt ggaccgcgcg ctggccgtgc
     1441 tgaccgcacg gcacgtgccg gcatgggtgc tcggtgaggt gcgcaggtcc cgcgatgtcg
     1501 gggtcgacgc taccgcgaaa gacgccgtcc gggccgtgct ccatggggat cacccacggt
     1561 tttgacccta atcaggcgtc tgcgccagca gcgtcgcggt caccggcggt gctggtcgta
     1621 cccgtcctcg tagagatcct cggtacggtc gtcactgcca cgatcctcgc tggcgtggcg
     1681 gccgccgttg ccgcccgaca gctctcgttg cagggcctcg agatcggtgg tgggggagct
     1741 gtacttgagc tcccgcgcta cctttgtctg cttggccttg gctcggccgc gccccatggc
     1801 tcgaccccct tgcacgcagg gacggggcgg ccgggggaat cggcggcctc gttcatctcg
     1861 acaacatcct ctaggtcaca ccgtacaggg ttgggcgggc tagatgcgac gcggccgcag
     1921 ccccccacag agtgtcccga aaatacggtc aggagtgtcg cgggcacccg cctgggcgct
     1981 cgtggcgacg tatgcaagac ttccgcagac cagaccgggg gcaggttcca cccgagagta
     2041 ggagttggca tgaccgtcct ggcgctcgtg accgccagcg gtgagccgct gtcttggctg
     2101 gccgtggctg tcgtgctact tggcacgacc ggactgatga tcggtactgc tcggcgggtc
     2161 aggaaccgag cttgagctgt gctcgtcggt gacccggcgc ttgttgaagg ccccgtgctg
     2221 gttggagacg tcgcgctgtt cggcgagtaa gt
//
LOCUS       DATADN010000485         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_368116, whole genome shotgun sequence.
ACCESSION   DATADN010000485 DATADN010000000
VERSION     DATADN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368116"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VHH53_06285"
     CDS             <1..365
                     /locus_tag="VHH53_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2289793.1"
                     /translation="VTLDGVTRHYTCARDGAVVWLGRDGHSWVLRELEGARRVARDVG
                     GAGPVLAPMPGTVTAVEVDEGQQVTAGARLLVIEAMKMEHVLTAPIDGVVRKLHARPG
                     GTVERDAVLAVVEPACTR"
     gene            459..1601
                     /locus_tag="VHH53_06290"
     CDS             459..1601
                     /locus_tag="VHH53_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2289794.1"
                     /translation="MELSEEHAALRATVEEFAHKEVAPVIGELYQRGEFPYELVAKMG
                     AMGLFGLPFPEEYGGMGGDYFALCLTLEELARVDSSVAITLEDSVSLGAMPIFRFGTE
                     EQKQRWLPELCAGHKLGAFGLTEPGGGSDARATRTTARCDEDEWVINGSKAFITNSGT
                     DITSVVTVTAITGVSNGRKEISTIVVPARHPGFTVSESYSKVGWNCSDTRELFFDDCR
                     VPQDHLLGERGRGYAQFLSILDEGRIAIAALGVGLAQGCIDECVSYARQRRAFGHAIG
                     SYQAIQFKIADMEVRAYTARLAYYEAAARMLRGQPFKKEAAIAKLVSSNAAMDNARDA
                     TQVFGGYGFMNEYPVARFYRDAKVLEIGEGTSEVQRMLIARHLGVG"
     gene            1666..2739
                     /locus_tag="VHH53_06295"
     CDS             1666..2739
                     /locus_tag="VHH53_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2289795.1"
                     /translation="MRVNYLTRPRENRLFRQCRRAWDLAAGERQHYEPVEPAQVFDFG
                     EAMHDALDVYYFPGMWEWNRAIVRPLAVAGFEKSMRRQRAAYAQTRDLSVDQVHDFDQ
                     HLELGARVLERYFEWALEVDRFTPIQVAAQFDINVPDPANPDSGLLTPDRRPIQFRVK
                     IDLAVIDDHELYWLVEHRIFTDPTWPDLDQLRLDEQALTRSWAWQLHFLAKLEGTIHN
                     ELRAASPDPAQTSEVAIGAVPGPGGIITQRSTESFRRIHLPRTELEIERRGVAVARET
                     QEMTDPSLRIYPTPSWEHCSACAYRSPCVVINQGLDEQPILQASYRQRVGEDFEPGRL
                     GSVWGFVPEVHRVAEHQPPGPRH"
     gene            2753..3484
                     /locus_tag="VHH53_06300"
     CDS             2753..3484
                     /locus_tag="VHH53_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289796.1"
                     /translation="MRLRVVSWNVDSRPTGLLDAKVELLRQLRPDLALLQEIGRPVYR
                     ALLPHPSAHQRMHLKSRLFSWGALSTDLYDRRASEYRLGCAVLGVPTTAVLDSSVLDR
                     APFDVRDPVRLGFLWRTVAARVALSTGALITVCSVHGRPGASPPPPELQRAFHAGIAE
                     WLATVPGPIIFGMDAGPSVLNGADPLLGSDPVHGLREVVRVSHDRLWATRDLEVLDIQ
                     CLADEATAAGSDHAVLLADLALRSS"
     gene            complement(3512..>3707)
                     /locus_tag="VHH53_06305"
     CDS             complement(3512..>3707)
                     /locus_tag="VHH53_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2289797.1"
                     /translation="LTPDRSAGGQRRYSRAELTLATRVRELLDQNMPLAAAIRIVSLE
                     HQLAAAHRRIGQLEQSVNER"
BASE COUNT          600 a         1198 c         1267 g          642 t
ORIGIN      
        1 tggtcacctt ggacggggtc acccgccact acacctgcgc gcgggacggc gccgtggtgt
       61 ggttgggccg cgacgggcac agctgggtgc tgcgcgaact cgaaggcgcg cggcgcgttg
      121 ccagggatgt tggtggtgcc ggccccgtgc tcgctccgat gcccggcacc gtcaccgccg
      181 tcgaggtcga cgagggccag caggtcactg cgggcgcccg gttgctcgtc atcgaggcga
      241 tgaagatgga gcacgtgctc accgccccga ttgacggggt cgtccgcaag ctgcatgccc
      301 gtcccggcgg gaccgtcgag cgcgacgcgg tgctcgccgt tgtcgaacca gcgtgcacga
      361 gatgacgaca gacggcgcaa gagagtgcga cacgcggtgc gtggggcggt gacatgccgg
      421 gacagaccgg gacaccgggc gggagcggag gacacaccgt ggaattgagt gaggagcatg
      481 cggcgctgcg tgccactgtg gaggagttcg cgcacaagga ggtcgctccg gtcatcgggg
      541 agctgtacca gcgtggtgag ttcccctacg agctggtcgc gaagatgggt gcgatggggc
      601 tgttcgggct gccctttccc gaagagtacg gcgggatggg cggcgactac ttcgcgctct
      661 gcctgacgtt ggaggaactt gcccgggtgg actcgtcggt ggccatcaca ttggaagaca
      721 gcgtgtcgtt gggcgcgatg ccgatcttcc ggtttggcac tgaggagcag aagcagcggt
      781 ggctgccgga gctgtgcgcc ggccacaagc tgggcgcctt cgggctcacc gagcccggtg
      841 gcggttccga cgcgcgcgct acccgtacca ctgcccggtg tgatgaggac gaatgggtga
      901 tcaacggctc caaggcattc atcaccaact ccggcaccga tatcacatca gtggtcacgg
      961 tcaccgcgat caccggcgtc agcaacggtc gcaaggagat ctccactatc gtcgttccgg
     1021 ctcggcatcc gggcttcacg gtttctgaga gttactccaa ggtcggctgg aactgttcgg
     1081 acacccgcga actgttcttc gacgactgcc gggtgccgca agaccacctg ctcggtgagc
     1141 gcggccgggg ctacgcccaa tttctgtcca tcctggatga gggacgtatc gcaatcgctg
     1201 cgctgggtgt cggtctggcc caagggtgca tcgacgagtg cgtgtcctac gcccggcaac
     1261 ggcgcgcttt cggccacgcc atcggcagct accaggcgat ccagttcaaa atcgccgaca
     1321 tggaggtgcg tgcgtacacc gcccggctgg cctactacga ggccgcggcg cggatgctgc
     1381 gtggccaacc gttcaagaag gaggccgcga tcgccaagct ggtctcctcg aatgcagcga
     1441 tggacaacgc tcgggacgcc actcaggtct tcggcgggta cgggttcatg aacgagtatc
     1501 cggtggcccg cttctaccgc gacgcgaagg tactggaaat cggcgagggc accagcgagg
     1561 tacagcgcat gctcatcgcc cgtcacctcg gcgtcggctg accctcctcc agcgaatgag
     1621 cccagggtag gctcgaattc ctggtgcggg cggcaggagg tagccgtgag ggtgaattat
     1681 ctgaccaggc cgcgggaaaa caggctgttc cgacagtgcc gcagagcctg ggatctcgcc
     1741 gccggggaac ggcagcacta cgagccggtc gagccggcgc aagtattcga cttcggcgag
     1801 gccatgcacg atgcgctgga cgtgtactac ttccccggca tgtgggagtg gaaccgggcg
     1861 atcgtgcggc cgttggccgt ggccggcttc gagaagtcga tgcgcaggca gcgggctgcc
     1921 tacgcgcaga cccgtgacct gtccgtggac caggtccacg acttcgatca gcacctggaa
     1981 ctgggtgctc gcgtcctgga gcgctacttc gaatgggcgc tggaggtgga ccgcttcacc
     2041 ccgatccagg tcgcagcgca gttcgacatc aacgttccgg acccggccaa tcccgacagc
     2101 ggtctgctca caccggaccg gcgccctatc cagttccgcg tcaagatcga cctggcggtg
     2161 atcgacgacc atgagctgta ttggctcgtc gagcaccgga tcttcaccga tccaacctgg
     2221 ccagacctcg accagctccg cctcgatgaa caggctctga ctcgcagctg ggcctggcag
     2281 ctgcacttcc tggccaaact cgaaggcacg atccacaacg agctgcgcgc agccagtccc
     2341 gacccggcgc agacctccga ggtcgcgatc ggagcggtac cggggcccgg tggcatcatc
     2401 acgcagcgca gcaccgaatc cttccgccgc atacaccttc ccaggactga acttgaaatc
     2461 gagcggcggg gtgtcgccgt tgcgcgagag acgcaggaga tgaccgatcc gtcgctgcgg
     2521 atctacccga ccccttcctg ggagcactgt tcggcctgtg cctatcggtc accttgcgtg
     2581 gtgatcaatc aggggctcga cgagcagccg atcctgcagg cgtcctaccg gcagcgcgtc
     2641 ggcgaggatt tcgagcccgg gcgcctgggc tcggtatggg gtttcgtccc cgaagtccat
     2701 cgggtcgcag agcaccagcc gcccggtcca cggcactgac cggctggctg cagtgcgctt
     2761 gcgagtggtg agctggaacg tcgactcccg accgaccggg ctgctggacg ccaaggtgga
     2821 actacttcgc cagctacggc ctgacctcgc gctgctccaa gagatcggcc ggccggtcta
     2881 ccgcgcgttg ctcccgcacc cgtcggcaca tcaacggatg cacctcaagt cgaggctgtt
     2941 ctcctggggc gccctgtcca ctgacctgta cgaccggcgg gctagcgagt accgcctcgg
     3001 atgcgctgtg cttggagttc ctaccacggc cgtgctggac tcctccgtgc tcgaccgtgc
     3061 cccattcgat gtccgcgatc ccgtgcggct cggattcctg tggcgaaccg tggcagcgcg
     3121 ggtcgccctg tccactggcg cgctgatcac tgtgtgcagc gtccacggtc gtccaggagc
     3181 cagccctccc ccgcccgagt tgcagcgtgc cttccacgcc gggatcgcgg aatggctcgc
     3241 caccgtgccc ggtccgatca tcttcggcat ggacgccggt ccatcggtac tcaacggcgc
     3301 ggacccgctg ctcggctcgg atccggtgca cggcctgcgt gaggtcgtgc gcgtaagtca
     3361 tgaccgcttg tgggccacca gggacctgga ggtactcgac atccagtgcc tagcggatga
     3421 ggccacagcg gcaggcagcg accacgccgt gttgcttgct gatctggcgc tgcgttcttc
     3481 ctgacgtggc cgcgcgctgg tgtggccagc cctagcgctc gttgacgctt tgttcgagtt
     3541 ggccgatccg ccggtgtgcg gcggccagct ggtgttcgag gctgacaatg cgaatggcgg
     3601 cagccagggg catattctga tcgagcagtt cgcgcacccg cgtggcgagg gtgagttcgg
     3661 cgcggctgta gcggcgttgg cccccagcgg agcgatccgg ggtcaag
//
LOCUS       DATADN010000486         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_42710, whole genome shotgun sequence.
ACCESSION   DATADN010000486 DATADN010000000
VERSION     DATADN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42710"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VHH53_06310"
     CDS             complement(<1..1088)
                     /locus_tag="VHH53_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX2289798.1"
                     /translation="MMAGDATRVLVDGTPLLGQRSGIGRYTAALLRELAIRTDVDVIV
                     TAFTARGQAALRGAVPPGVVVRGGPVPARALRALWQRFSWPPTELLAASANVLHATNF
                     VLPPSRRAHGVVTVHDLAFLDRPENLDPQQRGLPDLARNSVARAAVVCTPSNAVAQQV
                     TSRLDIPAQRVVVTPLGVERAWFSAAAPTEALRTMLGLPPRYLLFVGTAQPRKGLDVL
                     LEAHASQPGLAPLVLAGPAGWGQALVTSSRVHSVGYLKETDLRCVVAGAAALVLPSRD
                     EGFGLPILEAMATGIPVVCSDLPALREITGGLATLVPPEDPAALATTLAGAGDAGWDP
                     VGAAARQAHAARYTWQACANATVRAYRQA"
     gene            complement(1085..>2271)
                     /locus_tag="VHH53_06315"
     CDS             complement(1085..>2271)
                     /locus_tag="VHH53_06315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2289799.1"
                     /translation="KSIGPELDWAQLVVLQGHVLEQIPSLKTSNHIVIVDIYDPMHLE
                     QLEQGRDLRDGQRALVVDAVTSVLSAQLRRGDFFLCASERQRHFWLGHLAAIGRLSPT
                     VYDADPTTRSLLAVAPFGLSGKPPQRTGPGLRDALGFGSREKVVLWAGGVYSWFDPLT
                     LLHAVHALRAEHDDLRLVFLGMRHPNPDVPEMGMAGQTRRLAQILGLTGEHVFFNETW
                     VPYADRQNWLLDADAGVTTHFEHVETTFAFRTRVLDYLWAGLPIVTTDGDAFAELVAA
                     EELGIVVPAEDPAALAAALQRVLYDDAFAAGCRDRIAAVAQRFTWEAVLAPLTGFCRH
                     PRPAADRLRGATPVAVGRRHGMAGTLRRDADLIRSYLAAGGPSEVVRRASGRLRRLTG
                     WG"
BASE COUNT          356 a          795 c          816 g          304 t
ORIGIN      
        1 gcttgtcggt acgcgcggac cgttgcgttc gcacaggcct gccaggtata gcgggcggcg
       61 tgtgcctgcc gggcggctgc gcctaccgga tcccagccgg catcgcctgc gccggccaac
      121 gtggtggcca gcgcagcagg atcctcgggc gggacgagcg tggcgagccc cccggtgatc
      181 tcccgcagcg ccggtaggtc cgagcacacc accggtatcc cggtggccat cgcctccagt
      241 atcggaaggc cgaaaccctc gtcgcgcgac ggcagcacca gcgccgctgc gccggcgacc
      301 acgcagcgca ggtcggtttc cttgaggtaa cccaccgaat gcacccgaga agatgtcacc
      361 agcgcttgac cccagccggc cggtccggcg aggacgagcg gcgcgagccc gggttgcgac
      421 gcgtgcgcct cgagtagtac gtcgaggccc ttccgcggct gggcggtgcc gacgaacaac
      481 aagtaccgcg gcggcaaccc cagcatggtg cgcaacgcct cggtgggcgc ggcagcgctg
      541 aaccaggctc gctcgacgcc cagcggggtg accacgactc tctgcgcggg gatgtcgagc
      601 cggctggtca cctgctgtgc caccgcgttg ctgggggtgc acaccaccgc ggcccgggcc
      661 accgagttcc tggccaggtc aggcaggccg cgctgctgcg gatccaggtt ctccgggcgg
      721 tccaggaagg ccagatcgtg cacggtgacg acgccgtggg cgcgtctgga cggcggcagc
      781 acgaagttgg tggcgtgcag cacgttggcg ctggcggcga gcagttccgt cggtggccag
      841 ctgaagcgct gccacaacgc gcgcagcgcc cgggccggta ccgggccacc gcgcaccacc
      901 acgccggggg ggaccgcgcc gcgtagagcg gcctgaccgc gagccgtgaa cgcggtgacg
      961 atgacgtcca catcggtgcg gatcgccagc tcgcgcagca gcgcggcggt gtagcgaccg
     1021 atcccgctgc gctgcccgag cagcggcgtg ccgtcgacca gcacgcgggt ggcgtcgccg
     1081 gccatcatcc ccagccggtg agcctgcgca accgccccga cgctcgccgg actacctcgc
     1141 tgggcccgcc agcggcgagg tacgacctga tcaggtcggc gtcacggcgc agcgtgccgg
     1201 ccatcccgtg acggcgtcca accgcgaccg gcgtggcgcc gcgtagccga tcagcagccg
     1261 gtcgcgggtg ccggcagaag ccggtcagcg gcgccagcac cgcctcccag gtgaagcgct
     1321 gggccaccgc cgcgatccgg tcccggcagc cggcagcgaa ggcgtcgtcg tagagcaccc
     1381 tctgcagtgc cgcggccagc gcagccggat cctcagcggg taccactatg ccgagctcct
     1441 cggcagcgac cagttcggcg aaggcgtcac cgtcggtggt gacgatcggc agtcctgccc
     1501 acaggtagtc cagcactcgg gtgcggaagg cgaaggtggt ctcgacgtgc tcgaagtgcg
     1561 tggtgacgcc ggcgtcggcg tcgagcagcc agttctgccg gtcggcgtag ggcacccagg
     1621 tctcgttgaa gaacacgtgt tcgccggtga gcccgaggat ctgggcgagc cggcgggtct
     1681 gcccggccat gcccatctcg ggcacgtcag ggttcgggtg gcgcatcccg aggaagacca
     1741 ggcgaaggtc gtcatgctca gcacgcagcg cgtgcacggc atgcagcaag gtcaacgggt
     1801 cgaaccagga atagacccct ccggcccaca gcacgacctt ctcccgcgac ccgaatccca
     1861 gcgcgtcccg cagacctggc ccggtgcgct gcggtggttt gccggacagc ccgaacggcg
     1921 ccactgcgag cagcgagcgg gtcgtcgggt cggcgtcgta gacggtgggg gacagccgcc
     1981 cgatcgccgc caggtgcccg agccagaaat gccgctgccg ttccgacgcg cacaggaaga
     2041 agtcgccgcg ccttagctga gccgacagca cggaggtgac cgcgtcgacg accagcgcgc
     2101 gctgaccgtc tctcagatcg cgaccctgct cgagctgctc gaggtgcatc gggtcgtaga
     2161 tgtcgacgat gacgatgtgg ttggacgtct ttaaagacgg tatctgctcg agcacgtggc
     2221 cttgaaggac caccaactgc gcccagtcca gctcgggacc gatcgatttg g
//
LOCUS       DATADN010000487         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_801278, whole genome shotgun sequence.
ACCESSION   DATADN010000487 DATADN010000000
VERSION     DATADN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801278"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VHH53_06320"
     CDS             complement(<1..257)
                     /locus_tag="VHH53_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289800.1"
                     /translation="MGVEHAGGVVDVVVLDYGSGNLRSAERALRRAGAQVEVTADVDA
                     ALSADGLVVPGVGAFAACMAGLLAVHGDRLVPAQLQPRPSTR"
     gene            335..862
                     /locus_tag="VHH53_06325"
     CDS             335..862
                     /locus_tag="VHH53_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289801.1"
                     /translation="MDSGFGVRTEDLMRHLADLVARRYEGAESWPDRLAVFHRAVELL
                     DPVVRGVLAEADTTFLENTGEITRHSVEQNDGSVGEHWKLSWPRQREAVSRDGGPVAP
                     VEVIAWFRRSFNHAHLRGSTAGDWPLQVTSAGDAERQIPIVQAIVEAELHQRIYEGRY
                     PVMPVAVTKYGLPPS"
     gene            complement(903..2000)
                     /locus_tag="VHH53_06330"
     CDS             complement(903..2000)
                     /locus_tag="VHH53_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2289802.1"
                     /translation="MTRRVVVVGADATGMSAASQALRRAGRDALEVVAFERGHHVSYS
                     ACGIPYWVAGDVAGADALIARNPDEHRANGIDLRVRTEVTEIGLDTRTVTVDDLDSDK
                     SYQVDFDDLVLATGAVPVRPNLPGVGAPGVYGLQTLDDGAALLAGLTGQPRGAVVVGG
                     GYIGIEMAEAMVRRGLAVTLVDQAAEPMTTLDPDMGALVREAMQGMGITVLTDTAVTG
                     FETGPDGRVIGANLGGGYATFPGIVGTAVSKVCDLEVSRTGLLEAEARAAGFLPVSVT
                     VQSTTRAGYYPGTTPITVKMIAERGTGRLLGAQLVGREGSGKRVDVCAVALWNEMTVE
                     EMISLDLGYAPPFSPVWDPVLVAARKAAAAV"
     gene            complement(2028..2171)
                     /locus_tag="VHH53_06335"
     CDS             complement(2028..2171)
                     /locus_tag="VHH53_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289803.1"
                     /translation="MIGVGLLALAGFLLGGVVSTWRTSRPLAVALGFCAVLAGVGAVA
                     WLL"
     gene            complement(2168..>2465)
                     /locus_tag="VHH53_06340"
     CDS             complement(2168..>2465)
                     /locus_tag="VHH53_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX2289804.1"
                     /translation="TLVHVAVDVSGRPYCVHTGEPDAMAGFVVGGNYPTVLNRHVFET
                     LAFHAGMAVHIRVLHGRDPHHVTEAQYKALARALRAAVEFDSRTCGVPSTKGVL"
BASE COUNT          430 a          900 c          796 g          339 t
ORIGIN      
        1 cgcgtagatg ggcgtggttg aagctgcgcc ggaaccaggc gatcaccatg caccgcgagg
       61 agcccggcca tgcaggcagc gaaggcaccg actccgggca ccaccagccc gtcagcggac
      121 agcgcggcgt cgacatcagc ggtcacctcc acctgcgccc cagcgcggcg caacgcgcgc
      181 tccgctgacc gcagattccc cgacccataa tccagcacaa ccacgtcgac caccccacca
      241 gcgtgctcta cccccattca gcgggctcaa cggggtaaac agggcgccca taccccgctg
      301 agcccgctga atgcgtcatc ctgtcgatgt ggagatggac agcggatttg gagtgcgcac
      361 cgaggatttg atgcggcact tggcggatct cgtcgcgcgt cgttacgagg gcgccgagtc
      421 gtggccggac cggttggccg tcttccaccg ggcggtcgag ttgctcgatc cggtggtccg
      481 gggtgtcctc gccgaggccg acaccacatt cctggagaac accggcgaga tcacccgcca
      541 cagcgtcgag cagaacgacg gcagtgtggg cgagcactgg aagctgtcct ggccccggca
      601 acgggaagcc gtgtcccgag acggcggccc ggtggcgccg gtcgaggtga ttgcctggtt
      661 ccggcgcagc ttcaaccacg cccatctgcg cggctcgacc gcaggggact ggccgctgca
      721 ggtgacgagc gctggcgacg ccgagcgaca gataccgatc gtgcaggcca tcgtcgaggc
      781 tgagctacac cagcggatct acgaagggcg gtacccagtg atgcccgtcg cggtgaccaa
      841 gtacggcctt ccacccagct gaaccgcagt cagggccatc gcttcgaccc tgcaagcagg
      901 tctcagaccg cggctgcggc cttgcgggct gctaccagca ccgggtccca aaccggagag
      961 aacggtggcg cgtaaccgag gtccaggctg atcatctcct cgactgtcat ctcgttccag
     1021 agcgcgacgg cgcacacgtc cacccgctta cccgagcctt cccggccgac cagctgcgca
     1081 ccgagcagcc gcccggtgcc gcgctcggcg atcatcttga cggtgatcgg ggtggtgccc
     1141 gggtagtagc cggcgcgggt agtcgactgc accgtcacgc tcaccggtag gaaccccgct
     1201 gcgcgtgctt cagcctccag cagcccggtc cggctgacct caaggtcaca gaccttgctg
     1261 accgccgtgc cgacgatgcc gggaaaggtg gcgtagccac cgcccaggtt cgcgccgatc
     1321 acccggccgt caggaccagt ctcgaagccg gtgaccgcgg tgtcggtgag cacggtgatc
     1381 cccataccct gcatcgcctc gcgaaccaag gcacccatgt ccggatcgag ggtcgtcatc
     1441 ggctccgcgg cttggtccac cagcgtgacc gctaagccgc gccgcaccat cgcctcggcc
     1501 atctcgatac cgatgtagcc cccaccgacc acgaccgccc cacgcggctg tccggtcagc
     1561 ccggccagca gggcggcacc atcgtcaagg gtctgcaggc catagacacc cggtgcgccc
     1621 acgccgggca ggttagggcg caccggcacc gcgccggtgg ccagcaccag atcgtcgaaa
     1681 tcgacctgat agctcttatc actgtcaagg tcgtcaacgg tcaccgtgcg ggtgtccagg
     1741 ccgatctcgg tgacttcggt acggacccgc aggtcgatcc cgttggcccg atgctcgtcg
     1801 gggttgcggg cgatcagcgc gtcggcaccg gcgacgtctc ccgccaccca gtacgggatg
     1861 ccgcaggccg agtaggacac gtggtggccg cgctcgaagg ccaccacctc cagcgcgtcc
     1921 cgacccgcac gccgcaacgc ctgcgaggcc gcactcatcc cggtcgcatc agcgcctaca
     1981 accaccactc gtctcgtcat gccggcatgc tcccgcgccg gatccgatca caacagccag
     2041 gcgacggcgc cgacaccggc cagtacggcg cagaaaccga gcgccacggc gagcggccgc
     2101 gaggtgcgcc aggttgagac cacaccgccg agcaggaaac cagccagcgc cagcaggccg
     2161 acaccgatca cagcacgccc ttggtggacg gcaccccgca agtgcgcgag tcgaactcga
     2221 ccgcggcgcg caacgcccgg gccagcgcct tgtactgcgc ctcggtgacg tggtgcgggt
     2281 cgcggccgtg caggacacgg atgtgcaccg ccatccccgc gtggaaggcc agcgtctcga
     2341 agacgtgccg gttgagcacc gtggggtagt tgcctcccac cacgaaaccg gccatcgcgt
     2401 cgggctcgcc ggtgtgcacg cagtacgggc gcccggagac atccaccgcc acatgcacga
     2461 gggtc
//
LOCUS       DATADN010000488         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1121115, whole genome shotgun sequence.
ACCESSION   DATADN010000488 DATADN010000000
VERSION     DATADN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121115"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..318)
                     /locus_tag="VHH53_06345"
     CDS             complement(28..318)
                     /locus_tag="VHH53_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289805.1"
                     /translation="MTATSLVAAFLVLLIASWNLWNGFEGVSRPSLSRQRWYSLGILA
                     AATFLLIVGPVAALTFHPLQGADLTESMALGAILVGLVAVCLATLHHVNRRL"
     gene            complement(339..1200)
                     /locus_tag="VHH53_06350"
                     /pseudo
     CDS             complement(339..1200)
                     /locus_tag="VHH53_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1356..>1628
                     /locus_tag="VHH53_06355"
     CDS             1356..>1628
                     /locus_tag="VHH53_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2289806.1"
                     /translation="MHAEEARTIGQRLREIRHWRGKSLRVVAELAGISESYLSLIERG
                     QRPLDRRSKLEALAAALEVAPGELTGAHELTSAQGMGEAHAAVVALR"
BASE COUNT          270 a          511 c          577 g          270 t
ORIGIN      
        1 ggtcgtcccg agcgcttgtt catccggtca cagcctgcgg ttgacgtgat gcagggtggc
       61 gagacacacg gccacgaggc cgaccaggat tgcacccagt gccatcgact ctgttagatc
      121 agcgccctgg agcgggtgga acgtcaaggc ggcgacggga ccgacgatga gtaggaacgt
      181 cgcggcggcc aggattccca gcgagtacca ccgctgcctg gacagggagg ggcggctgac
      241 gccctcgaag ccattccaca ggttccacga cgcgatcagc aacacgagga aggctgcgac
      301 cagcgaggtt gctgtcatgg ctagagcgtg tctcccaatc atggtcgggc gaggatgacg
      361 gtcgccgcga gtaggactcc gccgagatag gtgcgggcgt acttgtcgta acgggtagcg
      421 atgccacgcc actgcttgaa ccggccgaac cctcgctcga cggtgttgcg ctgcttgtag
      481 aggtcggggt cgaacttggg gggtcggcca ccggctgagc ccttggattt gcggtgttgg
      541 acctggtcgg tgcgttctgg gatggtgtgg cggattcggc ggcgacgtag ttcgcgccga
      601 gtggaggggt ggctgtaggc cttgtcggcc agcaggtgct cggcgcgatg actggtctgg
      661 gcgagggtgt cgagcagcgg cacgaggcgg gggttgtctc ctgcttgacc ggccgagagc
      721 agcacggtga ccgggcgaac cccgccatcg accagcgcat ggattttggt ggtcagtccg
      781 ccccgggagc gacctatccc gtgatcatca ggttcgggaa tcttgtcatt cggctgagcc
      841 ccctgtgagg ctcgcgcggg gtgcgccagc ggcgtgctga tgggcgcgca cgatggtgga
      901 atcgaccgac agcagccagt ccagcttgcc cgcgttgtct tgcagcgcca gcacctgctc
      961 gaagagccgc tgatacgttc catccgttga ccagcggtga tgccgcttcc atagcgtctg
     1021 ccacggcccg aattcggccg gcacatcccg ccacgggcag ccggtccgaa accgccaggc
     1081 gatcgcttcg agcacctgac ggtgatcacg ccacggacga ccctgacgac cgacgcccga
     1141 aggcaacgcc ggctctacca gctcccacaa ctcatcggta atcacaccag tccggatcac
     1201 ccagcgatga tcgacctacc caccatcatt ctttgggaga cacgctctag ccgtcgggag
     1261 ggtggcccgt agcctggagt cacctcctcg ccgaggttcc gccccggggt cgctccccgg
     1321 ggcatcggcg gggagccggc gaggaaggtg ccgcgatgca cgctgaggaa gcacgcacaa
     1381 tcggccagcg gctgcgcgag atccggcact ggcgcggcaa gtcgttgcgg gtggtcgccg
     1441 agctcgcggg catctccgag tcttacctgt cgctgatcga gcgcgggcaa cgccccttgg
     1501 accgccgctc gaagcttgag gcgctcgccg cagcgctgga ggtggcaccc ggggagctga
     1561 ccggagccca tgagttaacg tccgcccagg gcatgggcga ggcgcacgct gcggtagtgg
     1621 cgctgcgg
//
LOCUS       DATADN010000489         2305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_662622, whole genome shotgun sequence.
ACCESSION   DATADN010000489 DATADN010000000
VERSION     DATADN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662622"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="tig"
                     /locus_tag="VHH53_06360"
     CDS             <1..896
                     /gene="tig"
                     /locus_tag="VHH53_06360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX2289807.1"
                     /translation="VSLDLTATIDGQEVDELATTGLSYQIGSGQLIEGIDDALIGASL
                     GEQRVFTTTLVAGPHVGQQAEVTAKVQAVKERELPAADDEFAQLASEFDTIDEVRADL
                     RERLTRVKAMQQGAQARDKVLEALLDSTEVPLPDSIVEAELQSRQHDALHAFDHDEQR
                     LSQWLEREGHTAEEFETDLRNAAQQAVKARLLLDAIADAEELSVTDDELSERIIYQAQ
                     RHGVSPNDYIKQVQEAGELGGMYADIRRGKALATVVRQATVTDAAGNSVDIEALVSSV
                     TPQRAESAGSPSLDDAGGNPA"
     gene            1089..1676
                     /locus_tag="VHH53_06365"
     CDS             1089..1676
                     /locus_tag="VHH53_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX2289808.1"
                     /translation="MRTASAGLNLTDSVYERLLRERIIVLGSQVEENIANQICAQLLL
                     LSAEDADQDIRLYINSPGGSVTAGMAIYDTMQLIEPDVATYAMGLAASMGQFLLSAGT
                     RGKRYALPHARILMHQPSAGVAGTAADIAIQADMLIKHKREMAELIAEQTGQPVERII
                     ADSDRDRWFTAEEAREYGFVDQVLTRANSLPDADR"
     gene            1776..>2305
                     /locus_tag="VHH53_06370"
     CDS             1776..>2305
                     /locus_tag="VHH53_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX2289809.1"
                     /translation="MPQERMPQSRYVLPSFIERTSYGVKESNPYNKLFEERIIFLGEQ
                     INNTSANDTMAQLLVLESTDPDRDITMYINSPGGSFTAMTAIYDTMQFVRPEIQTVCL
                     GQAASAAAVLLAAGTPGKRMAVPNAQILIHQPATEGVYGQVSDLEIQAAEIQRMRRLL
                     ESTLAHHTGQTEERIRA"
BASE COUNT          435 a          783 c          731 g          356 t
ORIGIN      
        1 tcgtgtcgct cgatctgacg gccaccatcg atgggcagga ggttgatgag ttggccacca
       61 ccggcctgtc gtaccaaatc ggctccggtc agctcatcga aggcatcgac gatgcgctga
      121 tcggggcctc gttgggtgag cagcgtgtgt tcaccaccac cctcgtcgcc ggaccccacg
      181 tcgggcagca ggccgaagtg actgcgaagg tccaggcggt gaaagagcgc gagctgcctg
      241 cggccgacga cgagttcgcc caactcgcca gcgagttcga caccatcgac gaggtccgcg
      301 cggacctgcg cgaacggctc acccgcgtca aggcgatgca gcagggtgcg caagcccggg
      361 acaaggtgct cgaggccctg ctggacagca ccgaggtgcc gctgccggac tcgatcgtcg
      421 aggccgagtt gcagtcgcgc cagcacgacg cattgcacgc cttcgatcac gacgagcagc
      481 gtctgagcca gtggctggag cgggagggcc acaccgccga agaattcgag accgacctgc
      541 gcaacgccgc gcagcaggcg gtgaaggctc gcttgttgct cgacgccatc gccgacgcag
      601 aggagctctc agtcaccgac gacgagctca gcgagcgcat catttatcag gcgcagcgcc
      661 acggcgtcag cccgaacgac tacatcaagc aagtccagga agcgggcgag ttgggcggca
      721 tgtacgccga catacgccgc ggcaaggcgc tggccacggt agttcgtcag gccaccgtca
      781 ccgacgctgc cggcaactcc gtggacatcg aggcgctggt cagttcggtg acgccgcagc
      841 gagcggagtc ggctggatca cccagtctgg acgatgcagg cggcaacccg gcgtgacgct
      901 ctgagcgaac ggagggcgcg tcaggactta gccaccgccc gccttcgtta gggtcggcag
      961 tgtccacagc tcgtgttcgg cgactctgag ccggaccctc aacccttacc ggacctcgtg
     1021 acagaaggca ggcatacgtg acctcggacc acatcgcctc ggccgtgccg ctgcgggacg
     1081 cccccgcgat gcggaccgcc tcggctggcc tcaacctcac cgactcggtg tatgagcggc
     1141 tgcttcgcga gcgcatcatc gtgctgggct cccaggtcga ggagaacatc gccaaccaga
     1201 tctgcgctca gctgctgctg ctgtccgcgg aggatgccga ccaggacatc cggctctaca
     1261 tcaactcacc cggtggctcg gtcaccgcgg gcatggccat ttacgacacc atgcagctga
     1321 tcgaacctga cgtggcgacc tacgcaatgg gtctggcggc ctcgatgggg cagtttttgc
     1381 tgtctgcggg cacccgtggc aagcgctacg ccctgccgca cgcccggatc ctcatgcacc
     1441 agccgtcagc gggtgtcgcc ggcactgcgg cggacatcgc gatccaggcg gacatgctga
     1501 tcaagcacaa gcgcgagatg gcagagctca tcgccgagca gaccggccag ccggtcgagc
     1561 ggatcatcgc cgactccgac cgcgaccgct ggttcaccgc ggaagaggcc cgtgagtacg
     1621 gcttcgtcga ccaagtcctg acccgcgcca attcgctgcc cgacgcggac cgctgagttc
     1681 gaggagccct catgaaccac ctgcccggag gcgctgtgcc ctacgatcgc atgcctcacg
     1741 accagatttc ccacggtcat ctgtccccgg aacgtatgcc ccaagaacgt atgccacagt
     1801 cgcgatacgt cctgccctcg ttcatcgagc gcaccagcta cggggtcaag gagtccaatc
     1861 cctataacaa gctgttcgag gagcggatca tcttcctcgg ggagcagatc aacaacactt
     1921 cggccaacga caccatggcg cagttgctgg tgctggagtc gaccgacccg gaccgcgaca
     1981 tcaccatgta catcaactca ccgggcgggt cgttcacggc gatgacggcg atctatgaca
     2041 cgatgcagtt tgtccgcccc gagatccaga cggtctgcct cggtcaggcc gcttcggccg
     2101 ctgcagtgct gctcgccgct ggcacccctg gcaagcggat ggcggttccc aacgcgcaga
     2161 tactcatcca ccagccggcc actgagggcg tgtacgggca agtatcggac ctggagatcc
     2221 aagctgccga gattcagcgg atgcggcggc tgctggagag cacgctggcc caccacaccg
     2281 gccagaccga ggagcgcatc cgcgc
//
LOCUS       DATADN010000490         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_802002, whole genome shotgun sequence.
ACCESSION   DATADN010000490 DATADN010000000
VERSION     DATADN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802002"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1595)
                     /gene="gltX"
                     /locus_tag="VHH53_06375"
                     /pseudo
     CDS             complement(103..1595)
                     /gene="gltX"
                     /locus_tag="VHH53_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671126.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
     gene            1651..>2131
                     /locus_tag="VHH53_06380"
     CDS             1651..>2131
                     /locus_tag="VHH53_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2289810.1"
                     /translation="MARCCVVGGGPAGMVLGLVLVRCGVEVVVLEKHADFLRDFRGDT
                     VHASTLALLDELGLGERFAAIPHRLIEQARVQLDSGTYTVGELSRLPGRHKHIALVPQ
                     WDLLDLLADAARDEPGFELRMSTEVTGLLRDGRGRVSGVRYRDRGDGGDGELAADLVV
                     "
BASE COUNT          340 a          706 c          762 g          323 t
ORIGIN      
        1 cggggcggaa ggctgaggag ggcttagctc gggcgcgaga gtcactcggt gcacgttaga
       61 tcgttagcag gccgcgccga ttctgctgac ccggtgaact tctcagccca gggactggtc
      121 actgtgcgct accagtcgcg cagcgctatg taaccggcgc agcgagcgat cccggcccag
      181 cacctccatc gactcataca gcggcgggga gacagtgtgc ccggtcaccg caacgcggac
      241 cggggcgaac gccttgcgcg gtggcagcgc caggccgtcg accaacgccg acttcagcac
      301 ctgctcgatg gccggcgcgc tccactctgg tgcctgctcc agtgcattaa tcgctgctcg
      361 caacaccggt acggcggccg gccccagcgc cttggccgct gcatcggggt caacagcgaa
      421 ctcgtcctcc gggatgagca ggaaacgcag catgcccacc gcatcggaca gcaccacgca
      481 ccgttcctgt accagcgggg caatcttgcg cagggtggcg ccgctggttc gattgatcac
      541 cagcccagca tctgctaggt aaggcaacac ccgggtgacg aactcctccg caggtaagga
      601 ccgcaagtgc acggagttga tcgcctcggc cttgcgcagg tcgaaccgcg ccgggttggc
      661 actgactcgg gacaggtcga acgccgccac cagttccgcc gtggtgaaca cgtcgcgatc
      721 ttcggcgatc gcccagccca gcagcgcgag gtagttcagc aggccttcag gtaggaaacc
      781 gcggtcgcgg tacacaaaca ggttggactg cgggtcacgc ttggacaact tgcgattgcc
      841 ctcccccagc acgtacggga ggtgaccgaa agccggggtg accgttgcca ccccgatccg
      901 ctcgagggcg gcgtagagcc caagctggcg tggcgtggaa ggcagcaggt cctcaccgcg
      961 cagcacgtgg gtgatccgca tcagcgcgtc gtccaccggg ttggtcaggg catacagcgg
     1021 ggagccgttg ccccggacca gtaccggatc aggcacggtg cccgatctga aggtgatctc
     1081 gccacggacc aggtcgatcc agctgagatc ctcatccggc atccgcaacc gaagcacggt
     1141 tagccgacct tcggagcgcc actgcgccgc ttgctgcgcg gtggtgtccc ggtcggcgtt
     1201 gtcgtagccc agcttggggt cgcggcgggc ggcgcggtgc cgcgcctcga tctcggccgg
     1261 cgtggcgtac gactcgtaca cctcgccggc ggcgaccagc cggtcgatca cgtcccggta
     1321 gatctcgccg cgctcgctct gccggtacgg gccgtaggcg ccgccgacct ggggccctcg
     1381 tcccagtcga ggccgagcca gcgcagcgcg tcgacgatct gctcgtagga ctgctcggag
     1441 tcgcggttgg cgtcggtgtc ctcgatgcgg aacacgaagg agccaccgtg atggcgggcg
     1501 tgtgcccagg agaacagcgc ggttcggatc agcccgacgt gcgggacgcc ggttggtgag
     1561 gggcagaaac gggtgcgaac ggacacggcg gtcagcgtat cggtgcggta gcctgaacca
     1621 aattcaacac ccgttgagtc ggaggccggg atggctcggt gttgtgtcgt cggcggcgga
     1681 ccggcgggca tggtgctcgg cctggtcctc gtgcgctgcg gcgtcgaggt ggtcgtgctg
     1741 gagaagcacg ccgacttcct gcgggacttc cgcggcgaca cggtgcacgc ctcgacgctg
     1801 gcgctgctcg acgagctcgg cctcggtgag cggttcgcgg ccatcccaca ccggctgatc
     1861 gagcaggcca gggtgcagct cgactccggc acgtacaccg tcggcgagct gagcaggctg
     1921 cccggcaggc acaagcacat cgcgctggtg ccgcagtggg acctgctcga cctgctggcc
     1981 gacgcggcac gcgacgagcc cggcttcgag ctgcggatga gcaccgaggt gaccgggctg
     2041 ctgcgcgacg ggcgcggccg ggtgagcggg gtgcgctacc gcgaccgtgg cgacggcggc
     2101 gacggcgagc tggccgcgga cctcgtggtc g
//
LOCUS       DATADN010000491         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1422119, whole genome shotgun sequence.
ACCESSION   DATADN010000491 DATADN010000000
VERSION     DATADN010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422119"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VHH53_06385"
     CDS             complement(<1..248)
                     /locus_tag="VHH53_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX2289811.1"
                     /translation="MGRVISTGGTELCCPREKVTARTVRPTVQAMLEQFEPAPAFVIN
                     HLADLLAWNDGYDRLVRPLGILVSERAPQEVGLVAVEDS"
     gene            502..1424
                     /locus_tag="VHH53_06390"
                     /pseudo
     CDS             502..1424
                     /locus_tag="VHH53_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
     gene            1476..1748
                     /locus_tag="VHH53_06395"
     CDS             1476..1748
                     /locus_tag="VHH53_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX2289812.1"
                     /translation="MRDRGCAFPGCSIPARWCEIHHVIHWADGGPTSVGNCVALCGRH
                     HHLIHHSDWRIQMTGGIPEFHPPPWLGGPPRRNPLHTSPELIRIRQ"
     gene            1799..>2198
                     /locus_tag="VHH53_06400"
     CDS             1799..>2198
                     /locus_tag="VHH53_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289813.1"
                     /translation="MSSNDQWSGAASDTGNILAGTPLGALVSGNSGFSIDYERVPQAI
                     ADLEHAAVFFTKQADVARELARIPAPGIDGVSLNAVAQIGRWASDVGTNNLEATLRAG
                     ATQLEDLARKLREDLMTYLEVEELNLPKPSP"
BASE COUNT          377 a          738 c          721 g          362 t
ORIGIN      
        1 gaatcctcga ccgcgacgag cccaacctcc tgtggtgcac gttcgctgac gaggattccg
       61 agggggcgca ccaaccggtc gtagccgtcg ttccatgcca ggagatcggc caggtgattg
      121 atgacgaagg ccggtgctgg ctcgaactgc tcgagcatgg cctgcacggt gggacgcacg
      181 gttcgtgcgg tcaccttctc ccggggacag cacagctcgg tgccaccggt gctgataacc
      241 cggcccaccc cgacaaacgc cgctgactct gctcactcag agcactgccg gcaccctgca
      301 aggtttttac gacgacgtag accgtgcctc gaaatatagc ttctagggtt ccagagcgtg
      361 gcgatccctg ttccgctaca gcgttcactg acatctccgg tgtcgaagta cacttcgcta
      421 gcgcggaatc tctctaaact ttgacatctg ggccccgaac gtaacgcggt ttttgtcggt
      481 gccccctcct agaattgggc catgtcgacg gcgccggtgt gcccagcagt gtggcagggc
      541 agtgataccg aattgctcgc cgagctgcgc gcgttggaaa tccggttgca ttccacctgg
      601 gcgcagatac tgtcagtcgt ggccgaaata gattcccggg gtatggccgc tgcagtggga
      661 tacggcacga ccgtggaact ggtgcgggcg gtcgcgcggg tgtcgcgggg tgaggcgcgg
      721 gcccgggtcg ccgccgccgc cgacgtgctc cccgggcagg gattgggcgg ggcgccggtg
      781 gcgccgcggc ttgcccagac cgcggcggcg gtggcacaac atgcgatcgg tgccgctgac
      841 gtcgcggtga tccgctcggt gctggcccac atcccgccgc atctcggcga ggacaagcgg
      901 gctgaggtag aggccgagtt agcccgccac gcccggacgt tggatgccgg gcagttggcg
      961 gtgttgggta agcggatcct ggcctacctc gatcaggacg gcccacgccc caaagacact
     1021 cccgagaccg ccggcgcttg agcttccatg accgcgacgg cggctatgag ctgaccggat
     1081 ggctggatcg ggaggccgct gagatcgtcc gctcagcgct gagcccattg gccgcacccc
     1141 gtccgaccac cgatatcgag gtcgacctgc gagataccgc gcagcgcgac gccgatgccc
     1201 tggtcgaact cgcccagcgc gcgctggaca gcggtgagct acccaccgag ggcgggcaac
     1261 gcccccaggt cgtggtgacc gtgagcctgc ccgtgctgca gggccggatc ggctcggcgt
     1321 cgctggccct gggcggccgg atcaacgccg acatcgctcg gcgcatcgcc tgcgacgccc
     1381 acatcatccc ggtagtactg ggtagccgag gtgagccgct agacgtcgga cgcgccagcc
     1441 acaccgtacc caccgcgatc cgccgcgcgg tgattgtgcg ggatcgcgga tgtgcctttc
     1501 cagggtgttc cataccagcc cgctggtgcg agattcacca tgtcatccat tgggccgacg
     1561 gcggaccgac cagcgtcgga aactgtgttg cgctctgcgg ccgtcaccat cacctcatcc
     1621 accattccga ctggcgaatc caaatgaccg gcggtatccc ggaatttcac ccgccaccat
     1681 ggctgggcgg gccaccacgc cgcaaccccc tccacacgag ccccgagctg atccgaatcc
     1741 gccaatagtg gcagactgct gcactcacca gcccaccaca cgtcaaggcg aacacgccgt
     1801 gagtagcaac gatcagtggt ccggcgccgc gagtgacacc ggcaacatcc tggccggcac
     1861 accgctgggc gccctcgtca gcgggaattc gggtttctcg atcgactacg agcgcgtccc
     1921 gcaagccatc gccgacctcg aacacgccgc ggtgttcttc acgaagcagg cggatgtggc
     1981 gcgtgagctg gcgaggattc ctgctccggg catcgatggg gtcagcctga acgcggtcgc
     2041 ccagattggc agatgggcgt cggacgtcgg cacgaacaac ttggaggcga ccctccgggc
     2101 tggcgccacg cagcttgagg atctggcgcg gaagctacgc gaggacctga tgacttacct
     2161 cgaggttgag gagctgaacc tcccgaagcc cagccctg
//
LOCUS       DATADN010000492         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_802291, whole genome shotgun sequence.
ACCESSION   DATADN010000492 DATADN010000000
VERSION     DATADN010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802291"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..918)
                     /locus_tag="VHH53_06405"
     CDS             complement(346..918)
                     /locus_tag="VHH53_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289814.1"
                     /translation="MQQIKGAILKSRLAFVEQHWGREGLDRVIASLPDEDQRVLRALL
                     TVKWYPFPLGERLDAAIVNVLGEGHTEVFERLGAASADVNLSTVHKQFLTPGRPHVFL
                     GKAPLIYGFYYETGRRTYQQTGERSGVMTTYEAETFSVPDCLTVIGWYRRALELCGVR
                     DVRIDEEECRAKGGAVCRYNIRWEDAPAQA"
     gene            complement(1036..1479)
                     /locus_tag="VHH53_06410"
     CDS             complement(1036..1479)
                     /locus_tag="VHH53_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289815.1"
                     /translation="MWIAALLLLGCGVGLVAVGWYSLTSYGLDPFEGGVLAPLSQRLL
                     MGAMFILPGLGVCALVIGYPLCYVTRIESNQARDEFRVRLLTGQELAVRLEDVVTAEF
                     KHGRFQNVNAPWYSVRLRGRRLPLMVDIQGELLDQEAADRLLADA"
     gene            complement(1612..2280)
                     /locus_tag="VHH53_06415"
     CDS             complement(1612..2280)
                     /locus_tag="VHH53_06415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2289816.1"
                     /translation="MRDAGRRLLGRGTDHRSIFTRIYRHNTWGSAESVSGPGSTRERG
                     ADFRDDLIALLRQLDARVLLDAPCGDFNWMEEVADSVTQYVGVDVVEEQLANNARRYS
                     APGRRFACADVTADPLPRADVILCRDCLVHFSFADAWAALENFRRSGSRWLLTTTFVD
                     RESNQDIRTGGWRTLNLQAAPFHFPPPVSVIDERCLHTGGIYRDKRLALWALDSLPAA
                     PASR"
BASE COUNT          461 a          974 c          948 g          429 t
ORIGIN      
        1 cgatgtcgga ccaggcgttc tgcgcgcgca ggcgcagccc caccagccgg ttgtacagcc
       61 cgatcacgag gatggcgagg agggcgagga cggccaggat gatccatgtc gtcatgcgag
      121 cgctccggcg aggggtgtgg cgcggaattc gtgattccta aggtaggccc acgcgtcccg
      181 agtgtaaaca cgcccgggat ttacctggtt cgcagaggaa agctgtttca cgcagaggtc
      241 gcagagctga aaagagagga cgcagagaac ttcggaagtt tctctgcgtc ctctcccgtt
      301 gtctccgcgc cctctgcgtg aaacgcgttt cgaaggcgca ttcgttcacg cctgagccgg
      361 tgcgtcctcc cagcggatgt tgtagcggca gacggcgccg cccttcgcgc ggcactcctc
      421 ttcgtcgatg cgcacgtccc gcacgccgca cagctccagc gcgcgccggt accagccgat
      481 cacggtcagg cagtcgggga cgctgaaggt ctccgcctcg tacgtggtca tcacccccga
      541 ccgctcgccc gtctgctggt aggtgcgccg ccccgtctcg tagtagaacc cgtagatcag
      601 cggcgccttg cccaggaaga cgtgcggccg cccgggggtc aggaactgct tgtgcaccgt
      661 ggacaggttc acgtccgccg aggcggcgcc cagtcgctcg aaaacctccg tgtgcccctc
      721 gcccagcacg ttcacgatgg cggcgtccag ccgctcgccc agcggaaacg ggtaccactt
      781 caccgtgagc agcgcccgta gcacgcgctg gtcctcgtcc ggcagcgacg cgatcaccct
      841 gtccagccct tcacggcccc agtgctgctc cacgaaagcc aggcggctct tcaggatggc
      901 gcccttgatc tgttgcatgg cggaagattc gggtggggtt ggatgcaccg ccgcgggcgg
      961 agtccagcgc caaagtggac ggaccgcaac gtatgtggtg cgccggatgg cgtcaagcgt
     1021 gttgtcggga cgcggtcagg cgtcggccag cagccggtcg gcggcttcct ggtccagcag
     1081 ctcgccctgg atgtccacca tcagcggcag ccggcggccg cgaagccgca cggagtacca
     1141 gggcgcgttc acgttctgga agcggccgtg cttgaactcc gcggtgacca cgtcctccag
     1201 ccgcaccgcc agctcctgcc ccgtcaacag ccgcacgcgg aactcgtcgc gcgcctggtt
     1261 cgactcgatc cgcgtgacgt agcagagcgg gtacccgatc accagcgcgc agacgccgag
     1321 tccgggcagg atgaacatcg cgcccatcag caggcgctgg gagagcgggg ccagcacgcc
     1381 cccctcgaac ggatccagcc cgtagctggt gagggagtac caccccacgg ccaccagccc
     1441 cacgccgcag ccgagcagga gcagggcggc gatccacacc aggatgagcc tgcgtgccga
     1501 gtagaccggg tgcatggcag gagaggttgc aggggaaccg ccacaggggg aaggaacgat
     1561 ttctttcgcc cgcccctgcc ggacgatgac gatggattcc cggcgccggt ttcagcgcga
     1621 ggccggcgcc gccgggagcg aatccagcgc ccacagcgcc agccgcttgt cgcggtagat
     1681 gccgccggtg tgcaggcacc gctcgtcgat cacgctcacc ggcggcggaa agtggaacgg
     1741 cgccgcctgc aggttcagcg tgcgccatcc gcccgtccgg atgtcctggt tggattcgcg
     1801 gtccacgaac gtggtggtca gcagccagcg cgatccgctg cgccgaaagt tctccagcgc
     1861 cgcccacgcg tccgcgaacg agaagtgcac caggcagtcg cggcacagga tcacgtccgc
     1921 gcggggcagc ggatcggcgg tgacgtccgc gcacgcgaag cgccgtcccg gggcgctgta
     1981 ccgccgcgcg ttgttcgcga gctgttcctc caccacgtcc acgcccacgt actgcgtgac
     2041 gctgtcggcc acctcctcca tccagttgaa gtcgccgcag ggcgcatcca gcagcacgcg
     2101 cgcatcgagc tggcggagca gggcgatcag gtcgtcgcgg aagtccgcgc cgcgctcgcg
     2161 cgtggagccg ggcccggaca ccgactccgc gcttccccag gtgttgtggc ggtagatgcg
     2221 cgtgaagatc gagcggtggt ccgtcccccg ccccagcagc cgcctgccgg cgtcgcgcac
     2281 caggtctgga agtgatttca cgggtgcatg agcgtaggct tcatcttttc cccgcccctg
     2341 tctgactgcg ggttcccgca tgggtctgga gtgctttcac gcctgccccc gctgtcatcc
     2401 tgagggagcg ccgctccaga ccattgtcac gcagggatgc ctgtgcgcga ccgaaggctc
     2461 tggcggcgta gcgcagggag cctgcccgtg cacggtcgta aaacccagcg cacgggcaag
     2521 atcctgcgct actccgacag atccttcggt cggcgccatg gattcagccc ggggcaggct
     2581 ctccgcggcg cctcccctca ggatgacagc acgggcaggc aggaaaacaa ctccagaccc
     2641 gatcgggaac agcgggtgag ggagcgccgc tccagaccat cgtcacgcag ggatgcctgc
     2701 gcgcgaccga aggatctggc ggcgtagcgc aggatcccgg gccgaaaaga aaagggctcc
     2761 agggcctcgc tgcgtcccct ggtcttcttc ccggccgttc aggagagtgg gc
//
LOCUS       DATADN010000493         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_43938, whole genome shotgun sequence.
ACCESSION   DATADN010000493 DATADN010000000
VERSION     DATADN010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43938"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..>4073
                     /gene="gltB"
                     /locus_tag="VHH53_06420"
     CDS             425..>4073
                     /gene="gltB"
                     /locus_tag="VHH53_06420"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX2289817.1"
                     /translation="MTANNLREGSSLRYSRRQAAGLYDAAYEHDACGVAFVADLAGRR
                     DHGIVSKALTALRNLEHRGARGGEPDTGDGAGILFQVPDRFFREVVDFELPEPGGYAV
                     GTAFLPADSTEMIETIERIAAEEGMGLLGWRELPVNPEGVGPTARSAMPAFRQLFLAA
                     AQDGGKPLTGLELERRAYCVRKRAERDADVYFASLSGRTIVYKGMLTEEQLVSFYPDL
                     SDERVTSSLALVHSRFSTNTFPSWPLAHPYRYVAHNGEINTLRGNRNWMHARESKLAT
                     DLIPGDLDRIYPIVTPEASDSASFDEVLELLHLGGHSMPHAVLMMIPEAWENHEEMDP
                     ARRAFYEFHSTLMEPWDGPALVAFTDGTVIGAVLDRNGLRPARYWVTEDGLVVLASEV
                     GVLDIDPATVVRKGRLEPGRMFLADTAAGRIVSDDEIKGQLAAEHPYAQWVAQGLTRL
                     QDLPDREREVHTHASLVRRQQTMGYTQEELEMLLTPMAVTGAEPIGSMGNDAPLAPFS
                     RLSRPLFDYFTQQFAQVTNPPLDAIREELVTSLHAQLGAQPNLLAADHDSCRRIVLPF
                     PVLDNDELAKIVHVNDDGDQPEYQTYTVQGLYDVHGGGAALVRRLDEIRAEVSAAVAG
                     GARLIVLSGRGVDAGHASIPSLLLTGAVHHHLVREKTRTQIGLIVETADAREVHHIAL
                     LIGYGAAAVNPYLAMATVEDLAERGLLNGVDGKTATRNLIKALGKGVRKTMSKMGVST
                     VASYTGAQIFEAIGLGAEVIDSCFTGTTSRLGGVGFDTLAEEVARWHGRAFPSDGVHA
                     PHRELAVGGDYQWRREGEPHVFNPQTVFKLQHSTRAGRYEIFKEYTRLVDDQSQRLMT
                     LRGLFAFREGVRPPVPIEEVEPVSDIVKRFATGAISYGSISKEMHEVLAIAMNRLGGK
                     SNTGEGGEDPDRYTVDPNGDWRRSAIKQVASGRFGVTSEYLVNADDLQIKISQGAKPG
                     EGGQLPGTKVYPWIAKTRYSTPGVGLISPPPHHDIYSIEDIKQLIHDLKNANPRARVH
                     VKLVSEVGVGTVAAGVAKAYSDVVLISGHDGGTGASPLSSIKHAGTPWVLGLAETQQT
                     LIANKLRDRIVVQTDGQLKTGRDVMIAALVGAEEYGFATATLVVSGCIMMRVCHLDTC
                     PVGVATQNPVLRAKFTGKAEYVVNFFEFIAQEVREYLAMLGFRTLADAIGHTEVLDTR
                     PAVEHWKAAGLD"
BASE COUNT          702 a         1375 c         1350 g          646 t
ORIGIN      
        1 atcacttcgc tcggcgacga tggtgatcgc taccgcgact cgcctggttc cccagcgcct
       61 gatcccgcgc cgcgggaagc gtcgcagcac atcccggatc tcctcaccca ggctcacgct
      121 gtgaggtcca aaccgggcac gtccaaaccg ggcacgtcca aaccgggaac cgccaaaccg
      181 ggggccttcc aatccgaacc acgcatcctt tcctggcagc cgccgttgcg gcgaccccga
      241 gcagggtgat tgtgcaccgc cgatggcgat ggccatcaca cgtccgggcg gctggctagg
      301 gatcgctggt caagaagtga taacggctcc ataccccgct gaggtaacct gcgggtagcc
      361 gcagatgggt ccatgtcgtc ccgatgcgtg tagcagctga aatcgagacg acggggaggt
      421 cctcgtgacc gctaacaacc tgcgtgaggg atcgagcctc cgatacagcc gacgtcaggc
      481 agcgggtctg tatgacgccg cttacgagca cgacgcctgc ggggtggcgt ttgtggccga
      541 cctcgccggg cgccgcgatc atggcatcgt cagcaaggct ctcactgcgc tgcgcaacct
      601 cgaacatcgg ggtgcccgag gcggggagcc cgacaccggt gacggtgccg gcatcctgtt
      661 ccaggttccg gatcgcttct tccgcgaggt tgtcgatttc gagctgcccg agccgggcgg
      721 gtatgcggtg ggtaccgcct tcctgccggc agacagcacc gaaatgatcg agaccatcga
      781 gcgcatcgcc gctgaggagg gcatggggtt gctgggctgg cgggagctgc cggtcaaccc
      841 cgagggagtc ggccccaccg cccgctcggc catgcctgcc ttccggcaac tgttcttggc
      901 ggccgcccag gacggcggca agcctctgac cgggttggag ctggagcgcc gcgcgtactg
      961 cgtgcgcaag cgtgctgagc gcgacgccga cgtctacttc gcaagcctgt ccgggcgcac
     1021 gatcgtctac aagggaatgc tcaccgagga acagctggtg agcttctacc cggacctgtc
     1081 cgacgagcgg gtcaccagct cgctggcgct ggtgcactcg cggttttcca ccaacacctt
     1141 tccgtcctgg ccgctggccc acccgtaccg ctacgtggca cacaacggcg agatcaacac
     1201 gctgcgcggc aaccggaact ggatgcacgc gcgggaaagc aagctcgcca ccgacctcat
     1261 ccccggggac ctggaccgga tctatccgat cgtcaccccg gaagccagcg actcggcatc
     1321 cttcgacgag gtcctcgaac tgctgcacct gggcggtcac tcgatgccgc acgcggtgct
     1381 gatgatgatc ccggaggcgt gggagaacca cgaggagatg gatcccgctc gccgagcgtt
     1441 ctacgagttc cattccacgt tgatggagcc ctgggacggc cccgcgctgg tcgcgttcac
     1501 cgacggaacc gtgatcggtg ccgtgctgga tcgcaacggc ctgcgcccag cccggtactg
     1561 ggtcaccgag gacggcctgg tggtgctggc cagcgaggtc ggggtgctgg acatcgaccc
     1621 cgccacggtg gtgcgcaagg gacggctgga gccgggccgg atgttcctcg ctgataccgc
     1681 agccggccgg atcgtcagcg acgacgagat caaaggtcag ctcgccgccg agcaccctta
     1741 cgcccagtgg gttgcccagg gtctgacccg gctgcaggac ctgcccgacc gcgagcgcga
     1801 ggtgcacacc cacgcgtcgc tggtgcgccg ccagcagacg atgggataca cccaagaaga
     1861 gctcgagatg ctgctgacgc cgatggctgt caccggcgcc gagccgatcg ggtcgatggg
     1921 caatgatgct ccgttggcac cgttctctcg gctatccagg ccgctgttcg actacttcac
     1981 ccagcagttc gcgcaggtca ccaacccgcc gctggacgcg atccgggagg agctggtgac
     2041 ctcgctgcac gcgcagctcg gtgcccagcc caacctgctg gcggccgacc atgactcctg
     2101 ccggcggata gtgctgccgt tcccggtact ggacaacgac gagctcgcca agatcgtcca
     2161 cgtcaacgac gacggtgacc agccggaata ccagacctat acggtgcagg gcctttacga
     2221 cgtacacggt ggcggcgcag cgctggtccg ccggctcgac gagatccgcg ccgaggtctc
     2281 cgccgcggta gccggcggtg ctcggctgat cgtgctctcc ggccggggcg ttgatgccgg
     2341 gcacgcatcg atcccgtcgc tgctgctcac cggtgcggtg caccaccacc tggtgcggga
     2401 aaagacccgc acccaaatcg gtcttatcgt ggaaaccgcg gacgcccgcg aggtgcatca
     2461 catcgcgctg ctgatcgggt acggcgcggc ggcggtcaat ccctatctgg cgatggccac
     2521 cgtggaggac ctcgctgagc ggggcctgct caacggcgtc gacggcaaga ccgctacccg
     2581 caacctgatc aaggcgttgg gcaaaggcgt ccgcaagacg atgtccaaga tgggtgtgtc
     2641 cactgtcgcc tcctacaccg gcgcgcagat cttcgaggcg atcgggctgg gagcggaggt
     2701 catcgactcc tgcttcaccg gtaccacctc ccggctcggc ggggtcgggt tcgacacgct
     2761 ggccgaagag gtcgcgaggt ggcacggccg cgctttccct tccgacgggg tgcacgctcc
     2821 gcaccgggag ctcgcggtcg gtggcgacta ccagtggcgc cgcgagggtg agccgcacgt
     2881 gttcaacccg cagacggtgt tcaagctgca gcactccacc agagccggtc gctacgaaat
     2941 cttcaaggag tacacccggc tcgtcgacga ccagtcgcag cggctgatga ccctgcgcgg
     3001 gctgttcgcc ttccgcgaag gtgtgcgccc gccggtgccg atcgaagagg ttgagccggt
     3061 cagtgacata gtcaagcggt tcgcgaccgg agccatctcc tatggatcca tttcaaagga
     3121 gatgcacgag gtgctggcca tcgcgatgaa tcgcctgggc ggcaagtcca acaccgggga
     3181 aggcggcgag gaccccgacc gctacaccgt cgatcccaac ggcgactggc gccgctcggc
     3241 gatcaagcag gtggcctccg gccggttcgg cgtcaccagc gagtacctcg tcaatgccga
     3301 cgacctgcag atcaagatct cgcaaggtgc caagcccggc gaaggtgggc agctgcccgg
     3361 taccaaggtg tacccgtgga tcgccaagac ccggtactcc acgcctggcg ttgggctgat
     3421 ctcgccaccg ccacaccacg acatctactc catcgaggac atcaagcagc tgatccacga
     3481 cctgaagaac gccaacccgc gggcccgcgt gcacgtcaag ctggtgtccg aggtcggggt
     3541 tggcacggtg gccgccggag tcgccaaggc gtactccgac gtggtgctca tctccggtca
     3601 tgacggtggc accggggcct cgccgctgtc gtcgatcaag catgccggca cgccctgggt
     3661 gctgggcctg gccgagaccc agcagacgct gatcgccaac aagctgcggg accggatcgt
     3721 cgtgcagacc gacggccagc tcaagaccgg ccgcgacgtg atgatcgccg cgctggtggg
     3781 tgccgaggag tacggcttcg ccaccgctac cctggtggtc tcgggctgca tcatgatgcg
     3841 ggtctgccat ctggacacct gcccggtggg ggtggccacc cagaacccgg tgttgcgagc
     3901 aaagttcacc ggcaaggccg agtacgtggt caacttcttc gagttcatcg cccaggaggt
     3961 ccgcgagtac ctggcaatgc tggggttccg cactctcgcc gacgcgatcg ggcacaccga
     4021 ggtgctggac acacgcccgg ccgtcgagca ctggaaggcc gccggactgg acc
//
LOCUS       DATADN010000494         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1422386, whole genome shotgun sequence.
ACCESSION   DATADN010000494 DATADN010000000
VERSION     DATADN010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422386"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VHH53_06425"
     CDS             <1..465
                     /locus_tag="VHH53_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HEX2289818.1"
                     /translation="VAVVREDGRWQCTPLDTAALRDLDAAITELRGQRSTGAVFGLLD
                     VDDEFFVVVRPVPGGVSLLLSDAVAALDYDVAADVLDLLRVEVPEEDTVDSDDVWPEG
                     DLGLLADLGLPAAEMQVIIDEVDLYPDEQLDMIAQRCGFGSQLSALLEKQPG"
     gene            462..974
                     /locus_tag="VHH53_06430"
     CDS             462..974
                     /locus_tag="VHH53_06430"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX2289819.1"
                     /translation="MTAVAGADAALVRRALKVASGAVATGDVPIGAVLVDADGVELAA
                     ACNAREATGDPTAHAELLVLRAGAARLGRDGRPDGWWLSGCTLVVTVEPCTMCAGALV
                     AARVRRLVFGAWEPKTGAVGSLWDVVRDRRSPHRPEVIGGVLADECAAMLTEFFAHHR
                     AAVAPIRPVW"
     gene            980..1069
                     /locus_tag="VHH53_06435"
     tRNA            980..1069
                     /locus_tag="VHH53_06435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Ser,seq:cga)
     gene            complement(1165..1743)
                     /locus_tag="VHH53_06440"
     CDS             complement(1165..1743)
                     /locus_tag="VHH53_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2289820.1"
                     /translation="MEAKELLDAASEVRNGAPFVIALTLGLRKGEALGLKWHDINFEQ
                     RTLTIRRTVQRLNWQHGCPPDKPCDRKYAGHCPQRHGGGASGRGGQIRSEQADDWVAA
                     AAYQSPDAASDSPATRTRKGARPLARGGLGIRQPARWPRASPSRPRRMEELAQDSRRA
                     RCLPLLPLLPMARLGAIDAGCSPSIATDETLL"
BASE COUNT          256 a          568 c          621 g          345 t
ORIGIN      
        1 gtcgctgtgg ttcgcgagga cgggcgctgg cagtgcacac cactggacac tgcggccctg
       61 cgagatctcg acgcggccat cactgaactg cgcgggcagc gctcaacggg agccgtcttc
      121 ggcctcctcg acgtcgatga cgagttcttc gtcgtcgtgc gacccgtgcc tggcggtgtg
      181 tcgttgctac tgtccgacgc cgtcgcagcg ctggactacg acgtcgccgc cgacgtgctg
      241 gatttgctgc gtgtcgaggt ccccgaggaa gatactgtgg attcagatga cgtctggccg
      301 gagggcgacc tcgggctgct cgccgacttg gggctgccgg cagcggagat gcaggtgatc
      361 atcgatgagg tggacctgta cccggacgag cagctcgaca tgatcgccca gcgatgtggg
      421 ttcgggtctc agctgtcggc gctgctggag aagcagccgg gatgacggcg gtggccggtg
      481 ccgatgcggc gctggttcga cgcgctctca aggtggccag cggcgcagtc gccaccggcg
      541 acgtgccgat cggggcggtg ctggtggacg ccgatggcgt cgagttggcc gcggcctgca
      601 acgcccggga ggcaaccggg gaccccaccg cgcacgccga gttgctggtg ctgcgggccg
      661 gtgccgcccg gctggggcgt gacgggcggc ccgacggttg gtggctgtca ggttgcacat
      721 tggtcgtcac cgtggagccg tgcaccatgt gtgcgggtgc cctggtggct gcccgggtcc
      781 ggcgcctggt gttcggcgcg tgggaaccca agaccggtgc cgtcgggtcg ctgtgggacg
      841 tcgtccggga ccgccggtcg ccgcaccgtc ccgaggtcat cggcggtgtt ctagcagacg
      901 agtgcgccgc gatgctcacc gagttcttcg ctcaccaccg cgcggcggtc gcgccaatac
      961 gtccggtatg gtgaccggcg gtagcgtgtc cgagcggccg aaggagcgcg cctcgaaagc
     1021 gcgtgagggt tcacgccctc cgtgggttca aatcccaccg ctaccgccat ctagcagcag
     1081 aaaagccggg caccctgatg ggccccccgg cggtccccgt ctcccgtctc agttttcgtc
     1141 tcgattgacc ctccgaccga gtgttcatag cagcgtctcg tccgttgcga tggagggaga
     1201 gcaacccgcg tcgatcgccc cgagacgggc catgggcaga agcggaagca gtggcaggca
     1261 tctcgcacgc ctgctgtctt gagcaagctc ctccattcgt cgtggtcgac tcggggatgc
     1321 acggggccac cgagccggtt ggcgaatacc caaccctcct cgtgccagag gtcgcgcgcc
     1381 ttttcttgtt cttgtagctg gcgaatccga tgctgcgtca ggactttgat aagcggcagc
     1441 ggcaacccaa tcgtccgctt gctcgctcct gatttgacct ccgcggccac ttgccccgcc
     1501 cccatgtcgt tgtgggcagt gaccggcgta cttacggtca cagggcttgt cgggcgggca
     1561 tccgtgctgc caattcagtc gttgcacggt ccggcgaatg gtcaatgtgc gctgctcgaa
     1621 attgatatcg tgccacttga gaccgagcgc ttcgccttta cgtaggccga gcgtgagagc
     1681 gatgacgaac ggagccccgt ttcgtacctc gctcgctgca tccaacagct cttttgcctc
     1741 catttgtgtg aacggtgtga tctcctcctc gggtatgcat ggcgggtcag
//
LOCUS       DATADN010000495         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_515956, whole genome shotgun sequence.
ACCESSION   DATADN010000495 DATADN010000000
VERSION     DATADN010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515956"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VHH53_06445"
     CDS             complement(<1..948)
                     /locus_tag="VHH53_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289821.1"
                     /translation="MPEAAVVRAASVVLAHTPGLARHGSKPMRTLAGDADLTASFLAN
                     LRSFDDAVGYPPHQAFLGACHPRELAPRPWVGTALAGAERFAPFGELMPEEEFLGLMA
                     AVDEFDLLVLAPEVAERAAKRLAEHPLAAQLELDRISAATGDVAAAVASGALPVHLAP
                     DCLAGALRGAHAEDESLAAHVLLENLACKATGTLALLHLLHDNSLDPAAVNYTISCSE
                     EAVGDRYQRGGGNMGKTIASVAGLTESSGADVKNFCAAPIPALVIAASLVVAGVFEQV
                     AVVAGGSLPKLGMKFEGHLHHGLPVLEDVIGGTAMLVTVD"
     gene            complement(941..2263)
                     /locus_tag="VHH53_06450"
     CDS             complement(941..2263)
                     /locus_tag="VHH53_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1226..1228),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEX2289822.1"
                     /translation="MRIVHYVNQFYAGLGGEEAAGTGPQVLDGPVGPGRLLGQLLNAE
                     YQDQYQIVATILCGDDYAASNAAATQELLEMARNAHAELLVAGPAFGSGRYGLACARL
                     VAAADAGGLPALAAMHPDNPGISDAGAAPVVAAGATSRQMRPTLQTVAAAIAKLATGA
                     ALTTADGRVARAARIGRLVEQRAAARAVELVLRRLGGDLESTEIPVGGFDVVDPAAPV
                     DKAASTTVALVTEGAVVPAGNPDRLESARATRWLRYQIDGLDTLVSGEWESVDGGFAT
                     TAANADPNRLLPLDTARALEREGAIGRLHNEFMGTVGNGTPVATARRFGVEWAAELRK
                     AGVQAAILTATUGTGTRCGSTLAKELERAGIPTALITNLVTIAEQVGAPRIVPGRGIP
                     YPSGDPNVPPPAEQAWRRQLVQRALDAVATAVERPTTFEVDVEERVDA"
     gene            complement(2276..>2748)
                     /locus_tag="VHH53_06455"
     CDS             complement(2276..>2748)
                     /locus_tag="VHH53_06455"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX2289823.1"
                     /translation="CFAGVVLCPEPVEQKDKELVSTHAAWLCRAAGFDAVIVTKEGAG
                     NADNDLMLKVDALEADGLTAVALYAELSGADGTGPPLVAGPRQGAVVSTGNYDQRVQL
                     PAVERALGSARLGIADAEATAAVEVPTAAILSALSPLGAGRLTCRAEQQSVGQQ"
BASE COUNT          409 a         1014 c          912 g          413 t
ORIGIN      
        1 gtcgaccgtc accagcatcg cggtgccccc gatcacgtct tccagcacgg gcagaccgtg
       61 gtgcagatgt ccctcgaact tcattcccag cttgggcagc gagcctccgg cgacgaccgc
      121 gacctgctcg aacactcccg ccaccacgag actggccgcg atcaccagtg cggggatcgg
      181 tgccgcgcag aaattcttga cgtccgcgcc cgatgactcg gtcagccccg cgaccgaggc
      241 gatcgtctta cccatgttgc caccgccacg ctggtagcgg tcgccgacgg cttcctcgga
      301 gcaggagatc gtgtagttca ccgccgctgg gtcgagactg ttgtcgtgca gcaggtgcag
      361 caacgccaga gtcccggtgg ccttgcaggc caggttctcc agcagcacgt gtgcggccag
      421 tgactcgtcc tcggcgtgcg cccctcgcag cgcaccggct aggcagtccg gcgccaggtg
      481 cacgggcaac gcgccgctgg cgacagcagc ggcgacatcc ccggtcgccg cggagatccg
      541 gtccagctcc agctgcgcgg ccagcgggtg ctcggccagc cgcttggcgg cccgctcggc
      601 gacctccggc gctaagacca gcaggtcgaa ctcgtccacc gcggccatca acccgaggaa
      661 ctcctcctcc ggcatcagct ccccgaacgg cgcgaaacgt tccgcgccgg ccaacgcggt
      721 cccgacccat gggcggggcg ccagctcgcg gggatggcaa gcgcccaaga acgcctggtg
      781 cggcgggtag ccgacggcat cgtcgaagct gcgcaaattg gccagaaagc tggccgtcag
      841 gtcggcgtca ccagccagcg tccgcatcgg cttggagccg tgccgggcca ggcctggagt
      901 gtgcgccagc acgacgctgg ccgcccgtac cactgctgcc tcaggcatcg acccgctcct
      961 caacgtcgac ctcaaaggtt gtcggacgct cgaccgcggt ggcgactgcg tccagcgcgc
     1021 gctgcaccaa ctgccgccgc catgcttgct cggctggcgg tggcacattg gggtcgcccg
     1081 acgggtaggg gataccccga ccaggtacga tgcgcggtgc gccgacctgc tcagcgattg
     1141 tcaccaggtt ggtgatcagg gctgtgggga taccggcccg ctccagctcc ttggcaagcg
     1201 ttgacccgca acgcgttccg gttcctcaag tggcggtcag gatcgccgcc tgcacgcccg
     1261 ccttgcgcaa ctccgcagcc cactcgacgc cgaatcgccg cgcggtggcc accggcgtgc
     1321 cgttgcccac cgtgcccatg aactcgttgt gcaaccggcc gatcgcgccc tcgcgctcca
     1381 gtgcgcgggc cgtgtccagc ggcagtagcc ggttcgggtc ggcattggcg gcggtggtcg
     1441 cgaagccgcc atccaccgat tcccactcgc cggagacgag cgtgtccagg ccgtcgatct
     1501 ggtagcgcag ccagcgggtg gcgcgtgccg actcaagccg gtccgggttc ccggctggca
     1561 ccacggcacc ctcggtgacc agcgcgacgg ttgtgctggc cgctttgtcg accggtgcgg
     1621 ccgggtctac cacgtcgaac cctccgacgg ggatctcggt cgactccagg tcgccgccga
     1681 gccgccgcag cacgagctcg actgcccgcg ctgcggctcg ttgctcgacc agccggccga
     1741 tccgggcggc ccgggccacc cggccgtccg ccgtagtcag cgccgcgccg gtggccagct
     1801 tcgcgattgc ggcggcaacc gtctgcaatg tcgggcgcat ctgtcgactg gtggcaccag
     1861 cggccacgac cggtgcggcg cccgcgtcag agatgccggg gttgtccggg tgcatcgcgg
     1921 ccagcgccgg caggcccccg gcgtcggcgg cggcgaccag ccgggcgcac gccaacccgt
     1981 agcgcccgct gccgaaggca ggcccggcaa ccaacagctc agcatgcgcg ttgcgagcca
     2041 tctccagcag ctcctgcgtg gccgctgcgt tggaggcggc gtagtcgtca ccgcacaaga
     2101 tcgtggccac gatctggtac tggtcctgat attcggcatt gagtagctgg cccagcagcc
     2161 gcccaggacc gaccggccca tcgagcacct ggggaccggt gccggcggcc tcttcgccgc
     2221 cgagcccggc atagaactgg ttgacgtagt gcacgatgcg catcaggctg tctccctact
     2281 gttggccgac cgactgctgt tcggcacggc aggtcagccg tccggcgccc agcggactca
     2341 aggcgctcaa gatcgcggcg gttgggacct cgaccgccgc ggtcgcctcc gcgtcggcga
     2401 tgccgagacg agcgctcccg agcgcccgct cgaccgccgg caactgcacc cgctgatcgt
     2461 agttaccggt actcaccacc gcaccttgtc gcgggccagc taccagaggt ggaccggtgc
     2521 cgtcagcgcc ggacaactcg gcgtacagcg ccaccgcggt cagaccatcg gcctccaggg
     2581 catcaacttt caacatcagg tcgttgtcgg cgttgcccgc accctccttg gtcacgatga
     2641 cagcgtcgaa gccggcggcg cggcacagcc aggcagcgtg cgtggacacg agttccttgt
     2701 ctttttgctc cactggctct gggcacagca ccaccccggc gaagcaca
//
LOCUS       DATADN010000496         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_44585, whole genome shotgun sequence.
ACCESSION   DATADN010000496 DATADN010000000
VERSION     DATADN010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44585"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VHH53_06460"
     CDS             <1..215
                     /locus_tag="VHH53_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289824.1"
                     /translation="DVTVEAWRVACGDCVHLSVTGELPGGTAVCVFGGVPFTGRGLGG
                     ELEPGRATVVSLATVRALATVGQVTA"
     gene            215..>1770
                     /locus_tag="VHH53_06465"
     CDS             215..>1770
                     /locus_tag="VHH53_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator"
                     /protein_id="HEX2289825.1"
                     /translation="MSDAQPNPLDLTQMTRAQRAALPLSTDVARAIAEQHGVCVRPLA
                     MRRIDTTTGRVDIVPVPCGSTRDDQCRPCADKARKLRMAQCRQGWHLDQEPATEQAAP
                     SDHHKNLIATRADLLAIYAECRAVGDEATCEQIADSVAELDTEIRASGIRGRLTPLDT
                     PAKPVRRSTQRRQDAPDLPRRRVERRTVGRVFAGRYRPSTFLTLTLDTYGRVDSHGAA
                     INPQAYDYRRAARDAIHFPALVDRFWQNTRRCVGWDVQYFGTVEPQKRGAPHFHAAIR
                     GAIPRAELRAITQATYHQVWWPAHDQLRYTDNRLPVWDSRARAFTDPATGIPLPSWEQ
                     ACQGLTGPAHVVRFGEQVHVKGILGGTEEAGRHIGYLTKYLTKSIGQTIGLNEHATDA
                     QRDHSHRLQAELRITPCSPRCAIWLLYGIQPKGARHSLTPGRCKGKAHQPEHLGIAGR
                     RVLVSRKWSNKTLHDHRTERGQFVRQLLEQAGIQPAHSPEDGPYLWERTAPADPDVPP
                     RPVLLLHAVA"
BASE COUNT          338 a          664 c          513 g          255 t
ORIGIN      
        1 ccgatgtcac cgtcgaagcg tggcgagttg cctgcggtga ttgcgtgcac ctgtcggtga
       61 ccggcgagct gcccggcggc accgcggtgt gcgtattcgg tggtgtgcca ttcaccgggc
      121 gtggtcttgg cggcgagctt gaacctggcc gcgccactgt cgtatcgctg gccactgtgc
      181 gtgccctggc caccgtagga caggtgacgg cctgatgagt gacgcccagc ccaatccgct
      241 tgacctcacg cagatgaccc gggcgcagcg agccgcgctg ccactgagca ccgacgtcgc
      301 ccgggcgatc gctgaacagc acggagtgtg cgtgcgaccg ctagcaatgc gccgtatcga
      361 caccactacc ggccgcgtgg atatcgtccc cgtcccctgc gggtccacac gcgacgatca
      421 atgccgaccc tgtgcggaca aggcccgcaa gctccgaatg gcccagtgcc gtcagggttg
      481 gcatctcgac caagagccag ccaccgagca ggctgcgccc agtgatcacc acaagaactt
      541 gatagccact cgggccgacc tactggccat ttacgccgaa tgccgcgctg tcggcgacga
      601 ggccacctgt gaacagatcg ctgattcagt cgccgaactc gacaccgaaa tccgagcatc
      661 aggcatacgc ggccggctca ccccgctgga cacaccggct aaaccggtgc gccgttccac
      721 ccaacgtcgc caagacgccc cggacctgcc ccggcgacga gtcgaacggc gcaccgtagg
      781 aagagtgttc gccggtcgct accggccctc cacattcctc accctcaccc tcgacaccta
      841 cggccgcgtc gactcccacg gcgcggcgat caacccccag gcctacgact accggcgggc
      901 agcgcgggat gcaatccact tcccggccct ggtggaccgg ttctggcaaa acacccgacg
      961 ctgtgtcggc tgggatgtgc agtacttcgg caccgtcgaa ccgcaaaaac gcggtgcccc
     1021 gcacttccac gccgccatcc ggggcgccat tccccgggcg gagttgcggg cgatcaccca
     1081 agcgacctat caccaggtgt ggtggcccgc ccatgaccaa ctccgctaca ccgacaaccg
     1141 gctccccgtg tgggactccc gggcgcgggc tttcaccgac ccggccaccg ggataccgct
     1201 gccaagctgg gagcaagcct gccaggggct gaccggaccc gcgcacgtgg tgcgcttcgg
     1261 ggaacaggtg cacgtcaaag gcatcctcgg cgggactgag gaagccggcc gccacatcgg
     1321 ctacctcacc aaatatctga ccaaatccat cgggcaaacc atcgggctca acgagcacgc
     1381 caccgatgct caacgtgatc actctcaccg gttgcaagcc gagctccgga tcacaccctg
     1441 ctccccgcgc tgcgcgatct ggctgctcta tggtatccaa cccaaaggag cccggcactc
     1501 cctgaccccc ggacgctgca aaggcaaagc ccaccagccc gaacatctcg gcatcgccgg
     1561 acgccgcgtc ctggtctccc gaaaatggtc gaacaaaacc ctgcatgacc accgcaccga
     1621 acgcggccaa ttcgttcggc aactcctcga acaagccggg atccaacccg cccacagccc
     1681 agaagacggg ccgtacctgt gggaacgcac cgcacccgca gatccagacg tgccaccacg
     1741 gcccgtcctg ctcctgcacg ccgtcgccga
//
LOCUS       DATADN010000497         2419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_663873, whole genome shotgun sequence.
ACCESSION   DATADN010000497 DATADN010000000
VERSION     DATADN010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663873"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..743)
                     /locus_tag="VHH53_06470"
     CDS             complement(3..743)
                     /locus_tag="VHH53_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEX2289826.1"
                     /translation="MTDTTEACSDLIEAVELARRAAQEEAGDSPVGPPVGPQTEDGGA
                     VTHLFEAGLPGYRGWRWAVTVASAGPATPVTVSEVVLLPGPDALVAPTWVPWTQRVRA
                     GDLGVGDLLPTDPDDPRLVPGYLIDDDPPAEQLSPELGLGRSRVLSRQARLALLQRWR
                     DSDRGPDSEMARSAPDRCVTCGFYLPLGGVLAASLGVCGNELAPADGQVVHAEFGCGA
                     HSEVQLDTSPSVPVAELVYDDAELEIVT"
     gene            813..1580
                     /locus_tag="VHH53_06475"
     CDS             813..1580
                     /locus_tag="VHH53_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX2289827.1"
                     /translation="MRAVATAAGLAAAVLVAAGCTTHSAADQLRVQVLATLPHDTTMY
                     TQGLEIHNGVLYEGSGLVGRSRVRATALSSAAVLREATLPAPLFGEGLTVAEDRLWQL
                     TWTSGTAIERDPATLAERRRVSYRGQGWGLCHDGQRLVMSDGSDRLTFRDPATFAPTG
                     HVLVRLDGEPARQLNELECAGGMVWANVYQDDRILRIDPGSGAVTGVVDASELLSASP
                     QPAAGVLNGIAAIPGTDEFLLTGKNWPSLFRVRFVPS"
     gene            1632..>2419
                     /locus_tag="VHH53_06480"
     CDS             1632..>2419
                     /locus_tag="VHH53_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2289828.1"
                     /translation="MTPPRSPFGGEHPGYRGARRVGRGRRWVPDDPGTSPTGTPPQSP
                     AWVPTAAPAPWGDRGASRWLPSDQYVSPPGDGSATAPVPESSGPPRPRKITVTRVVAW
                     RSRQLVGGGVRRFHQATTADGAGRSGLSSLTYATMADYAASAAIAMALANTLFFAAAT
                     AQSRANVALYLLITVAPFAVVAPVIGPLLDRLQHGRRVAMSLSFAGRALLLIVMATHV
                     NDWLLYPAALGFMVLTRSFGVLKAAVTPRLLPPQITLVKVNSRLT"
BASE COUNT          352 a          860 c          846 g          361 t
ORIGIN      
        1 gttcaggtca ctatctccag ctcggcgtcg tcgtagacca gctcggccac cggaacgctg
       61 ggactggtgt ccagctgaac ctcggaatgc gctccgcagc cgaactcggc gtgcaccacc
      121 tgcccgtcgg cgggagccag ctcgttgcca cagacaccga gggacgcggc gagcaccccg
      181 ccgagcggaa ggtaaaagcc gcatgtcacg catcgatcgg gggcgctgcg ggccatttcc
      241 gagtcggggc cgcgatcgct gtcgcgccat cgttgtagca gcgccaggcg agcctgccgg
      301 ctcagcaccc gggatcgtcc cagaccgagc tccgggctga gctgctcggc cggcgggtca
      361 tcgtcgatga gatagccggg aaccagccgt gggtcatccg ggtcggtggg cagcaggtca
      421 cctaccccga gatcgcccgc ccggacccgc tgggtccagg gcacccaggt cggcgcgacc
      481 aatgcgtccg gaccgggcag cagcaccacc tcgctgaccg tgaccggagt cgcgggacct
      541 gctgaggcca ccgtgaccgc ccagcgccag ccgcggtagc cgggcagccc agcctcgaac
      601 aggtgggtga cggcgccgcc gtcctcggtc tgcggtccga ccggcgggcc caccgggctg
      661 tcgcccgcct cctcctgcgc cgcgcgccga gccagctcga cggcctcgat gaggtcggag
      721 catgcctcgg tggtgtcggt caccccccga ttgtgccgca cccgcagtcc gcgccgacga
      781 cccggccagc gggtctgtgc gacgctgcat ccatgcgggc cgttgcgacc gccgccgggt
      841 tggcggctgc agtgttggta gctgccggct gcaccacgca ttcggccgcg gaccagctgc
      901 gcgtccaggt cttggccacg ctcccgcacg acacgacgat gtacacccag ggcttggaga
      961 tccacaacgg cgtgctctac gagggcagcg ggctggtggg gcggtcccgg gtacgggcca
     1021 ccgcgctgtc cagcgctgcc gtgctgcgag aggcgacgct gccggcacct ctgttcgggg
     1081 agggactcac cgtggcagag gatcgattgt ggcagctgac ctggaccagc ggaacggcga
     1141 tcgagcgtga tccggcaact ctggccgagc gccggcgggt gagctaccgc ggccagggat
     1201 ggggcctgtg ccacgacgga caacggctgg tgatgagcga cggctcggac cggctcacct
     1261 tccgggaccc ggcaaccttt gcccccaccg gccacgtgct ggtgcgcctc gacggcgaac
     1321 cggcgcgtca gctcaacgaa ctggaatgcg cgggcggaat ggtctgggcc aacgtctatc
     1381 aggacgaccg catcctgcgc atcgaccccg gctccggcgc ggtgaccggc gtggtcgacg
     1441 catccgaact gctcagcgcg tcaccacagc ccgcagcggg cgtgctgaac ggcatcgcag
     1501 cgatcccggg caccgacgaa tttctcctca ccggcaagaa ctggccgtcg ctgttccggg
     1561 ttcgcttcgt cccgtcttga cggcacccag ggcatggcgt tcaggcgcta ccggtggtgc
     1621 aggatgagca cgtgacaccg ccccgatccc cattcggtgg cgagcatccc ggctaccggg
     1681 gggcccgccg ggtgggccgc gggcggcgct gggtacctga tgaccccggt accagcccaa
     1741 ccggtacccc accgcagtcc ccggcctggg taccgacggc cgcgcccgca ccgtggggcg
     1801 accggggcgc cagccgctgg ctacccagcg atcagtatgt ctcgccccca ggagacggct
     1861 cggcgacggc cccggtgccc gaatcgagcg ggccgccgcg cccgcgcaag atcaccgtta
     1921 cccgggtggt tgcctggcgg agcaggcagc tggtcggagg tggcgtgcgg cgcttccacc
     1981 aggccaccac ggccgatgga gcgggacggt ccgggctgtc ctcgttgacc tacgcgacga
     2041 tggcggacta cgcggcgagc gcggccatcg ccatggccct ggccaacacg ctgttcttcg
     2101 cggcagccac cgcgcagagc cgggccaatg tggcgctgta tctactgatc acggtggcac
     2161 cgttcgcggt agtcgcgccg gtgatcggcc cgttgctgga ccggctgcag cacggcaggc
     2221 gggtagcgat gtcgctgtca ttcgccggtc gcgctttgtt gttgatcgtg atggccacgc
     2281 acgtcaacga ttggttgctc tacccggcag cgttgggttt catggtgctc accaggtcct
     2341 tcggtgtgct caaggcggca gtgacgccgc gcttgctccc gccccagatt accctcgtga
     2401 aggtcaactc gcgactcac
//
LOCUS       DATADN010000498         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1423188, whole genome shotgun sequence.
ACCESSION   DATADN010000498 DATADN010000000
VERSION     DATADN010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423188"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VHH53_06485"
     CDS             complement(<1..611)
                     /locus_tag="VHH53_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2289829.1"
                     /translation="MTSSLAGTSLALGPSWLDPDQLLRMLGPYVLVGLCLIIFAECGL
                     LIGFFLPGDSLLFTAGLFVANGIIGAPIWLVCLLLTVCAVVGNIVGYFIGFKVGPALF
                     NRPDSKLFKREHVDKTHAFFEKYGPRAIVLGRFVPIVRTFITAIAGVGRMEPRCYFTY
                     SVIGGVAWATGVTLLGFWLGRITFVKDNVEVVLIGIVMLSVLP"
     gene            complement(661..885)
                     /locus_tag="VHH53_06490"
     CDS             complement(661..885)
                     /locus_tag="VHH53_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289830.1"
                     /translation="MNGDQVPRRLAAALRAQASGLGTGPSTGSVPDARQVPGHPPARG
                     WRGLPVWGVLALVVLLGALAGGLAGVISVW"
     gene            complement(938..1510)
                     /locus_tag="VHH53_06495"
     CDS             complement(938..1510)
                     /locus_tag="VHH53_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX2289831.1"
                     /translation="MTLGHLRVLDALPGVPTGAAADAPADGTVEAPLTLEDLYRQHRM
                     RLVRLAILLVDEPATAEDVVQEAFTGLHRHWAGLRDEAAALGYLRTAVVNGSRSVLRR
                     RKTARGYVPPHQANARSAESLAMLTTEHQSVVAALGQLPPRQREVLVLRYYGGMSEAE
                     IATATGVSRGTVKSSASRALDVLQRILEQR"
     gene            1697..2731
                     /gene="fbaA"
                     /locus_tag="VHH53_06500"
     CDS             1697..2731
                     /gene="fbaA"
                     /locus_tag="VHH53_06500"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX2289832.1"
                     /translation="MPIATPEVYSEMLDRAKADQFAYPAINVTSSEKLNAALRGLAEA
                     GSDGIVQLSTGGSEFASGSKVKDMVVGSVAMAEFASVVAPRYPINIVLHTDHCPENKL
                     DTFVRPLIAISQERVARGGDPLFQSHMWDGSATELHRNLAIAAELLESCAKAKIILEV
                     EIGVVGGEEDGVANDINEKLYTTAEDFEHTVDALRGGEHGRYLLAATFGNVHGVYKPG
                     NVKLRPEILQMGQEVAARKLGLPAGSKPLDLVFHGGSGSQLAEIHQALSYGVIKMNVD
                     TDAQYAFTRPIAAHMFTNYDGVLKVDGEVGNKKVYDPRSYLKVAEQSMAARVVQACED
                     LRSAGRTLAG"
     gene            2843..3259
                     /locus_tag="VHH53_06505"
     CDS             2843..3259
                     /locus_tag="VHH53_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HEX2289833.1"
                     /translation="MSVHGNLLGPNPTLLPEQPDAEAALAAANDPATAAAAHPEFSAA
                     WAALAERALDEGDAVAGYAFARTGYHRGLDHLRRAGWKGHGPVPWSHGPNRGFLRALA
                     ALARAAAEIGEDGEARRCRDFLDECDPVAADALGLG"
     gene            complement(3383..>3569)
                     /locus_tag="VHH53_06510"
     CDS             complement(3383..>3569)
                     /locus_tag="VHH53_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289834.1"
                     /translation="VVRVVGRGLLGRRAVRQAAGRAVQLGCVPTDVWRDGVADRTTER
                     WTWYRHTEDWQLIRIPP"
BASE COUNT          639 a         1229 c         1186 g          515 t
ORIGIN      
        1 atcggtagga ccgacagcat cacgatgccg atcaggacca cctccacgtt gtccttgacg
       61 aacgtgatcc ggcccagcca gaagccgagc agggtgactc cggtggccca ggcgacgcca
      121 ccgatcaccg agtaggtgaa gtaacagcgg ggctccatcc ggcccacccc ggcaatcgcg
      181 gtgatgaagg tccgcacgat aggcacgaaa cgccccagca cgatggcccg cggcccgtat
      241 ttctcgaaga aggcatgtgt cttgtccacg tgttcacgct tgaacagctt cgaatccggc
      301 cggttgaaca gcgctgggcc caccttgaag ccgatgaagt agccgacgat gttgcccacc
      361 acggcgcaca cggtgagcag caggcagacc agccagatag gggcgccgat gatgccgttg
      421 gcgacgaaca gcccggcggt gaacagcagt gagtcaccag ggaggaagaa gccgatgagc
      481 aggccgcatt cagcgaaaat gatgaggcac agaccgacca gcacgtacgg gcccagcatc
      541 cgcagcaact gatccgggtc cagccaggag gggccaaggg ccagcgacgt gccggcaagg
      601 gaggacgtca ccgcgtcacg gtaccgccca cctcagacat ggccggcgcg gcgcccgccg
      661 tcaccagacg ctgatcaccc cggccagccc acctgccagc gcgcccagca gcaccaccag
      721 cgcgagcacc ccccaaaccg gtaacccgcg ccagccccgc gccggaggat gacccggtac
      781 ttgccgggca tccggtacag accccgtcga tggcccggtg cccaggccgg aggcttgggc
      841 ccgcaacgcc gcggccagcc gccgcggcac ctgatcgccg ttcacggcga ccccatgagt
      901 ggcattgagt gtgataacac tgttacgcac tcacgagtca ccgttgctcc aggatccgtt
      961 gtaagacgtc tagggcgcgg ctggcgctgg acttcacagt gccgcggctg actccggtgg
     1021 ccgtcgcgat ctccgcttcg ctcatccccc cgtagtagcg cagtaccagc acctcacgtt
     1081 gtcgcggtgg cagctgaccc agcgccgcca ccaccgactg gtgctccgtg gtgagcattg
     1141 ccaacgactc cgcggagcgt gcgttggcct ggtgtggcgg gacgtaaccg cgggcggtct
     1201 tgcgacggcg cagcaccgaa cgggatccgt tgaccaccgc agtgcgcaga tagcccagcg
     1261 cggcggcctc gtcgcgcagt cccgcccagt gccggtgtag cccggtgaac gcctcctgca
     1321 cgacgtcctc tgcggtggcc ggttcgtcca ccagcaggat cgccagccgc accagccgca
     1381 tccggtgttg ccggtagaga tcctccaggg tcagcggtgc ctcgacggtg ccgtcggctg
     1441 gggcgtcagc tgcggcaccg gttgggacac cgggcagggc gtccagcact cgcaagtgac
     1501 cgagggtcat ctcgaccgag tgctgcacgg ttgcgcggtc cagcggttcg tcgggggcca
     1561 caccggaaac gtactgtggg cacgtcgggc aggcaccgcg cccacccggt cgctacttgg
     1621 gtgcgccata ctgccgctca actcttgccg gggcacagcc ccagccgaca gtagcgagta
     1681 ccggaggcca gtcaccatgc ccatcgcgac acccgaggtc tacagcgaga tgctggaccg
     1741 cgcgaaggcg gaccagttcg cctatccggc catcaacgtg acctcgtcgg agaagctgaa
     1801 cgcggccctg cgcgggttgg ccgaggccgg cagtgatggc atcgtgcagc tgtccacggg
     1861 cggatcggag ttcgcctccg gcagcaaggt caaggacatg gtcgtcggct cggtggcgat
     1921 ggccgagttc gctagcgtgg tggccccgag atacccgatc aacattgtgc tgcacaccga
     1981 ccactgcccg gagaacaagc tcgatacctt cgtccggccg ctgatcgcga tttcccagga
     2041 gcgggttgcc cgtggcggtg acccgctgtt ccagtcacat atgtgggacg gctcggccac
     2101 cgagttgcac cgcaatctcg ccatcgctgc agagttgctc gaaagctgcg ccaaagccaa
     2161 gatcatcttg gaggtggaga tcggtgtcgt gggtggcgag gaggacggtg tcgccaacga
     2221 catcaacgag aagctctaca ccaccgccga ggacttcgag cacaccgtcg acgcgctgcg
     2281 cggcggcgag catggccgct acctgctggc cgccaccttc ggcaatgtcc atggcgtgta
     2341 caagccgggc aacgtgaaac tgcgcccgga gatcctgcag atggggcagg aggtcgccgc
     2401 tcgtaagctc ggtctgcccg ctggctccaa gccgctggac ctggtatttc atggcggatc
     2461 cggctcgcag ctggcggaga ttcatcaggc gctctcgtac ggggtgatca agatgaacgt
     2521 cgataccgac gcccagtacg cgttcacccg tccgatcgcc gcccacatgt tcaccaacta
     2581 cgacggagtg ctcaaggtcg acggcgaggt cggcaacaag aaggtctacg acccacggtc
     2641 ctacctcaag gtggccgagc aatcgatggc cgcccgcgtt gtgcaagcct gcgaggacct
     2701 gcgctcagcg gggcgcaccc tggccggtta ggggcccttc agaaccacca accccctcgt
     2761 cgctgccctc aacggctacc aaccttgacg gcggtgtgcg gcgctgtcgg cggtccacgc
     2821 ggcagcacgg caggatggag tgatgagcgt gcacggcaac ctgctgggac ccaatccgac
     2881 gttgctgccc gagcagcccg acgccgaggc ggctctcgcg gcggccaatg acccggcgac
     2941 ggcggccgca gcgcaccctg agttctcggc cgcctgggcg gcgctggccg aacgggcgct
     3001 cgacgagggc gacgccgtcg ccggctacgc cttcgcccgc accggttacc accgcggcct
     3061 ggaccatctc cgccgcgcgg gctggaaagg tcacggacca gtgccgtggt cacatggccc
     3121 taaccggggt ttcttgcgtg cacttgccgc gctggcccgg gcggccgccg aaatcggcga
     3181 agacggcgag gcccggcggt gccgggactt cctcgacgag tgtgatccgg tcgcggccga
     3241 cgctctggga ttgggctgat gacccagcga ccgcgcagaa ggtcgcgaca caccgggcaa
     3301 gagcgccgga cacaccgggc atgagagctc gacacaccgg gcatgagagc cggacacgcc
     3361 ggggttgggt tggggttgag cgttagggcg ggatccggat cagctgccag tcctcggtgt
     3421 gccggtacca ggtccagcgc tcggtagtcc ggtccgccac gccgtcacgc catacgtcgg
     3481 taggcacaca gcccagctgc acggcacgcc cggcggcctg gcgaaccgca cggcgcccga
     3541 gcagtccacg ccccaccacg cggaccacc
//
LOCUS       DATADN010000499         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_972945, whole genome shotgun sequence.
ACCESSION   DATADN010000499 DATADN010000000
VERSION     DATADN010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972945"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VHH53_06515"
     CDS             <1..856
                     /locus_tag="VHH53_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX2289835.1"
                     /translation="SFKSTTRVNVPSRLKNQAYPFLGNVELGAITPTMVRNWIRWMKD
                     RGTAQSYRHVCFVHVSAILNAAVDDKKIAVNPCQARSITKPTPGLHKIVPWTDARLKA
                     VWLALPPIVQVTMPLGAGAGLRQGEMFGLSPLDIDREGKVIHVVRQVQQVNNALMFCL
                     PKREKMRDVPLSDSTLAQLDHYMAMFPPVPITLPWEEADGTPTTVDLIMTDESGRAWW
                     RQTFNQKWWKPALERAGVESATRTDGTHALRHYYASALLEGGVSIKAVSEYLATPTRA
                     SLYGHTRT"
     gene            complement(977..2410)
                     /locus_tag="VHH53_06520"
     CDS             complement(977..2410)
                     /locus_tag="VHH53_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEX2289836.1"
                     /translation="MSFASPLFLWYFMPAVLAAVLIAPRGWRNGVVATASLAFYAVAA
                     GPTTLLLLGCMVVNYLMGPALEPDDWDLEPQRRRRWLCGVVAFNVAVLVVWKYAGFAS
                     AQAAGLASFTGIDLPVVELVLPIGISFYTFHHISYMVDVYRGERRALRDPLSFAAYIT
                     MFPQLVAGPIVRYREIADQLGRSRSHRLDDIAAGFPRFALGLTKKVVIADSLAPVVDA
                     CFNTPADQMTSSIAWLGAIGYTLQLYFDFSGYSDMAVGLARMLGFTLPENFARPYSAA
                     SITEFWRRWHMSLSRWLRDYVYIPLGGNRHGGATTYRNLWIVFALAGLWHGANWTFLI
                     WGLFHGTVLVIERRHGWDVAAAEPGQWLARRVLTLLIVVFGWVLFRSPDLGTALVFVQ
                     HMVIFDPGGLTDVVDASLTNQRLAILLVAALVFLLPARPVTGPYLESARSRPATVLRV
                     GVMTVGLFYAALLLATGTFSPFLYYRF"
     gene            complement(2486..>2705)
                     /locus_tag="VHH53_06525"
     CDS             complement(2486..>2705)
                     /locus_tag="VHH53_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289837.1"
                     /translation="VDRLQARGLVARVQDNADRRYTRISVTEKVDRYVAEMEAGAFGR
                     LATALGNATSEQRRTISDGLALLRQLLS"
BASE COUNT          583 a          869 c          858 g          395 t
ORIGIN      
        1 gtcatttaag tcgacaaccc gagttaatgt cccgtcgcgg ctgaagaacc aggcgtaccc
       61 gttccttgga aacgtcgaac tgggtgcgat cacgccgacc atggtccgca actggatccg
      121 gtggatgaag gaccgaggca cggcacaaag ctaccgacac gtgtgctttg tccatgtatc
      181 agcgatcctc aatgcggcgg tcgatgacaa gaagattgcc gtgaacccat gccaggctcg
      241 gagtatcacc aagcccactc caggactgca caagatcgtg ccgtggacag acgcccgact
      301 caaggctgta tggctggcgc taccgccgat cgtccaggtc acgatgcccc tcggcgccgg
      361 tgccggcctg cgccaaggcg agatgttcgg gctctcaccg ctcgatatcg atcgggaagg
      421 aaaggtcatc cacgtagttc ggcaggtaca gcaggtcaac aacgcactga tgttctgcct
      481 gcccaagcgg gagaagatgc gagatgtgcc gttgtcggac agcacgttag cccagctgga
      541 ccactacatg gccatgttcc caccagtccc gatcaccctg ccctgggaag aagccgacgg
      601 aacacccacc acagtggacc tcatcatgac cgacgagagc ggccgggcct ggtggcggca
      661 gaccttcaac caaaaatggt ggaagccagc gctcgaacgg gccggagtcg agagcgccac
      721 gcgtacagat ggcacacacg cactgcgcca ctactacgcc tcagcgctac tggaaggcgg
      781 cgtgagcatc aaggccgtgt ctgagtacct ggccacgccg acgcgggctt cactctacgg
      841 acatacacgc acctgatgcc ggccagccac gatcggagcc ggcgggtgat cgattcgcgc
      901 ctcgggcggt ctgacggcct ggagacggcc tgcgagcctg ttgatcagaa aaatcccagt
      961 tcagaggcgg tgtggattaa aaccggtagt agaggaaggg gctgaaggtt ccagtagcga
     1021 ggagcagcgc ggcgtagaac agaccgacgg tcatcactcc cacccgcagc actgtggcgg
     1081 gcctggatcg tgccgactcc agatacggac cggtgaccgg ccgggcgggc agcaggaaca
     1141 ccagggcagc cacaagcagg atcgccagcc gctggttggt caaggaggcg tccacgacgt
     1201 cggtcagccc gccgggatcg aaaatcacca tatgctgcac gaataccagt gcggtgccga
     1261 ggtccggtga tcggaacagt acccagccga acaccacgat gagcagggtc agcacccggc
     1321 gcgctagcca ctggccaggc tcggccgccg cgacgtccca cccgtggcgg cgttcgatga
     1381 ccagcaccgt gccgtggaac agaccccaga tcaggaacgt ccagttcgcg ccgtgccaga
     1441 gcccggccag cgcgaacacg atccacaggt tcctataggt ggtcgcacct ccgtgccgat
     1501 tgcccccgag cgggatatac acgtagtcgc gcaaccatcg ggacagcgac atgtgccagc
     1561 gccgccagaa ctcagtgata ctcgccgccg aatacgggcg cgcgaaattc tccggcaggg
     1621 tgaacccgag cattctcgcc agtccgacgg ccatgtctga atatccggaa aagtcgaagt
     1681 acagctgcaa ggtgtagccg atcgctccca accaggcgat cgagctggtc atctggtcgg
     1741 ccggagtgtt gaagcaggcg tccacgaccg gcgccagcga gtccgcaatc acgaccttct
     1801 tggtcagacc gagagcgaaa cgcggaaacc ccgcggcgat gtcatccagc cggtggctgc
     1861 gcgaccgccc gagctggtcg gcgatttcgc ggtagcgcac gatcggccct gcgacaagct
     1921 gagggaacat cgtgatatag gcggcgaacg agagcggatc gcgcaacgct cggcgttcac
     1981 cgcggtagac gtccaccatg taggagatgt ggtggaaagt gtagaaggag atcccgatcg
     2041 gcagcaccag ctcgacgacc ggcagatcga ttccggtgaa cgatgccagc ccggcagcct
     2101 gcgcgctggc gaagccggcg tacttccaca ccaccagcac cgcgacattg aaggcgacga
     2161 cgccacacag ccaccggcgg cggcgttgtg gctcgagatc ccagtcatca ggttccagcg
     2221 ccgggcccat caggtagttg acgaccatgc accccagcag caacagggtc gtcgggccag
     2281 ccgcgaccgc gtaaaaggcc aggctggcag ttgccacgac gccattgcgc cagcccctgg
     2341 gcgcgatcag cacggcggca agcacggccg gcatgaagta ccacaggaac agtgggctgg
     2401 cgaacgacat ggcggcggcg cagcctagcc gagcacaacc gcgccaacgc tggcccctcg
     2461 cgggcaagag cgtcgagtaa aaaattcagc tcagcagttg ccgcagcagg gcaaggccgt
     2521 cagaaatcgt gcgacgttgc tcgctggtgg cgttgcccaa cgcggtagcc agccggccga
     2581 acgctccagc ttccatctcc gcgacgtacc ggtccacttt ctcggtgacc gagatgcgcg
     2641 tgtagcgacg gtcggcattg tcttggaccc gcgcgacgag gccgcgcgcc tgcagccggt
     2701 caacg
//
LOCUS       DATADN010000500         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_803253, whole genome shotgun sequence.
ACCESSION   DATADN010000500 DATADN010000000
VERSION     DATADN010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803253"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..447
                     /locus_tag="VHH53_06530"
     CDS             64..447
                     /locus_tag="VHH53_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX2289838.1"
                     /translation="MRMTPQRQLVLDAVRDMEHATPERICEHVQHRAPAVNITTIYRT
                     LDLLEGLNLVRHTHLGHGAPSYSTQPHEHVHLVCHRCGEITEIPTEELTELAERLRQR
                     FSFVLDPSHLALSGRCANCIGQDGS"
     gene            444..1478
                     /locus_tag="VHH53_06535"
     CDS             444..1478
                     /locus_tag="VHH53_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEX2289839.1"
                     /translation="MTSPLLDLPGAVPPPAGSPDAGVAWHHGDPLGEQRAAERAAVVV
                     DQSHREVLAVPGEDRLSWLHTITSQHLLALGEGEHTEALVLDLQGRVEHHAVTSHVGE
                     TVWLDTEPGKAEALLAYLTSMVFWSKVAPRDATSELAVLSLLGPATDKVIAQVGQLGG
                     FVRVRPEQTDLLVPRGDVTDIWQRLVEAGATPAGSWAYEAFRVAALRPRLGLDTDERT
                     IPHEVGWIGSAVHLDKGCYRGQETVARVANLGRPPRRMVLLHLESPETLPSTGDPVRA
                     DQRVVGRVGTVATHHELGPIALALLKRSVDPSTPLLAGESAASVDPDSIPPDTGEPPG
                     RAALRRLKSR"
     gene            1504..2127
                     /locus_tag="VHH53_06540"
     CDS             1504..2127
                     /locus_tag="VHH53_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEX2289840.1"
                     /translation="MIEVRPGGHRRIDRVLDPGYVADLSELALAEVRARRDDAAQEET
                     DLSYLRRLLHARIDIVRAEQRRRHEGGNAPVVADLARILSAHALGPAIGSGRFQPLEP
                     SHAHRRTIDALVSDVDLSDVGSLSVEQLDTALQAYSTQEKSVSQRRREVQHVMDTFNA
                     EIGGRYASGAASVDDLLAEQRRVTDQHSSSPVPDPTSSTDHQSGRAE"
BASE COUNT          413 a          862 c          814 g          391 t
ORIGIN      
        1 ctgtctaggc ataccatcgt gctcgtggat acctccctgc gccgcacgct gcacgagcgg
       61 ggaatgcgga tgactcccca gcggcaactg gtgctcgacg cagtgcgaga catggaacac
      121 gccaccccgg agcggatctg cgaacacgtg caacaccgcg cacccgcagt caacatcacc
      181 acgatctacc gcaccctcga cctactcgaa ggcctcaacc tggtccggca tacccacctt
      241 ggacacggcg caccgagcta ctccacccag ccacatgaac acgtacacct ggtctgccac
      301 cgttgtgggg agattaccga gatacccacc gaggagctca ccgaactcgc cgagcggttg
      361 cggcagcggt tcagtttcgt tctggatccc agccatctgg cgctgtccgg acggtgcgcg
      421 aactgcatcg gccaagacgg ctcgtgactt cacccctgct cgacctgccc ggcgcggtcc
      481 ccccgcccgc cggctccccc gacgccgggg tggcctggca tcacggcgac ccgttgggtg
      541 agcagcgcgc cgccgagcgc gccgccgtag tcgtggacca gtcccaccgc gaggtgctcg
      601 ccgtgccggg tgaagaccgg ttgagctggc tccacaccat cacctcccag cacctgctcg
      661 cactcggcga gggcgagcac accgaggcgc tggtactgga tctccagggt cgcgtcgaac
      721 accacgctgt gacctcccac gtgggcgaga cggtgtggtt ggataccgaa cccggcaagg
      781 ccgaggctct gctggcctac ctgacctcga tggtgttctg gtcgaaggtg gcgccgcggg
      841 atgcgactag cgagctggcc gtgctctcac tgctcggtcc ggccaccgac aaggtgatcg
      901 cgcaggtcgg acagctgggt ggctttgtgc gcgtccggcc agagcagaca gacctgttgg
      961 tgccgcgcgg tgatgtcacc gacatctggc agcggctggt cgaagccggt gccactcctg
     1021 cgggtagctg ggcctatgag gccttcaggg tggcggcact gcgcccgcga ctgggcctcg
     1081 acaccgacga gcgcaccatc ccgcacgagg tcggctggat cggctcggcg gtgcacctgg
     1141 acaagggttg ctaccggggt caggagacgg tggctcgggt ggcgaacctg ggccgcccgc
     1201 cgcggcggat ggtcctactg cacctggaat cacctgagac actgcccagc accggggatc
     1261 cggtgcgcgc ggatcagcgg gtggtcggcc gggtcggcac cgtagccacc caccacgaac
     1321 tcggcccgat cgcgctggca ttgctcaagc gctcggtgga tccgagcacg ccgctgctgg
     1381 caggggaatc cgctgcgtcg gtggatccgg attcgattcc tccggacacc ggagaaccac
     1441 caggccgggc tgcactacgc cggctgaaat cccggtaggc cggtcggaag gatggtaggt
     1501 cccgtgatcg aggttcgtcc cggtgggcac cgtcgcatcg atcgggtgct ggatcctggc
     1561 tatgtcgccg acctgtccga gttggcgctg gccgaggtcc gcgcgcgccg agacgacgcg
     1621 gcgcaggagg agaccgacct gtcatacctg cgccggctac tgcatgcccg gatcgacatc
     1681 gtgcgcgccg agcaacgacg ccggcacgag ggcggcaacg ctcctgtcgt ggcggatcta
     1741 gcccggatac tctcggccca cgccctgggc ccggcgatcg gctcgggccg cttccagccc
     1801 ctggagccct cgcacgcgca ccgccgaacc atcgatgcac tggtttccga tgtcgacctc
     1861 tctgatgtcg ggtcgctttc ggtcgagcag cttgacaccg ctttgcaggc ctacagcact
     1921 caggagaagt cggtatccca gcgtcgccgt gaggtgcagc acgtaatgga taccttcaat
     1981 gctgaaatcg gcggccggta cgccagcggt gctgcctcag tggacgactt gctcgccgaa
     2041 cagcgccggg tcaccgacca gcacagctca agcccggttc ctgacccgac gagcagtacc
     2101 gatcatcagt ccggccgtgc cgagtagcac cacaaccacc gctagccaag acagcggctc
     2161 accgctggcg gtcacgagcg ccaggacagt catgccaact cctcgctgtc gagtggaacc
     2221 tgcacccgat ctggtcggcg gaagtcttgc acacgtcgcc acgagcgcct gggcgggtgc
     2281 ccgcgacact ctcaggtcct atttttcggg acactctgtg gggggctgcg gccacgtcgc
     2341 atccggcccg cggaaccctg tacggtgtga cccagaggat gttgtcgaga tgaacgaggc
     2401 cgccgattcc cccggccgcc ccgtccctgc gtgcaagggg gtcgagccat ggggcgcggc
     2461 cgagccaagg ccaagcagac
//
LOCUS       DATADN010000501         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_44889, whole genome shotgun sequence.
ACCESSION   DATADN010000501 DATADN010000000
VERSION     DATADN010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44889"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..424)
                     /locus_tag="VHH53_06545"
     CDS             complement(41..424)
                     /locus_tag="VHH53_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289841.1"
                     /translation="MGRESLLACRSVLHRILQFAEDEGAIASNPVRKVPPPKRRAFTP
                     EEAGRLLGRVPLFWWDHVLTFLGTGVRFGELAGLRCRRVHLDRPVPVLQVVEVRYQAG
                     GEFGSGFKSRPKSDAGIRELPLAHW"
BASE COUNT          317 a          569 c          587 g          271 t
ORIGIN      
        1 gggttggtgt ggggtggcag ctgtcgccgg atggactcga tcaccagtgg gcaaggggga
       61 gttcccggat gccggcgtcg ctcttgggac gtgacttgaa gccgctgcca aactcgccgc
      121 cggcttggta gcgcacctcg acgacctgga ggacgggcac gggccggtcg agatggaccc
      181 ggcggcagcg caggccggcc agctcaccga agcgcacgcc agtgccgaga aaggtcagga
      241 cgtgatccca ccagaacagt ggaacgcggc cgagcagtcg gccagcctct tcgggggtga
      301 aggcgcggcg cttcggcggt ggcaccttgc gcaccgggtt gctggcgatg gcgccctcgt
      361 cctcggcgaa ctgcaggatg cgatggagga cggaccgaca ggcgagcagg gattcgcggc
      421 ccaccttggc tgtgagttgg gcctgccagc gccgaacctc ggccgggctg acactgctcg
      481 atcgggcggt cgccaaacca tggccggata tggcgtcgca ggcggtcctc ggttgccgcc
      541 aacgtggtgg gactgcggtc cgggtcagcg gcccacacct cccaccagtc gtctgcccat
      601 tgatcgaacg agagtcggcc gctacgtcgt cctcgccagg tgccccggcg catttccaag
      661 cgtttctcgg cggcgaggtc acgggcctcg tcaggggtgt cgaccgtctt gccgccttgg
      721 gagccgtcat caagtcgcca ggagaccaga taccggttcc cgcgcttgcg ccaactcacc
      781 gaccccatca gggtctctcc gcatggaacg gaggaataac gttctggaga ccgggctgtc
      841 gaggcgcgca ggcggggcgc tggcggctgt cggcggctag gcgcgactcc tgacatggcc
      901 gggaacggct ggggctgttc tcgttggata acgtgacagc cgcccggact cgacatcgtc
      961 acggacgacc tcattgccca attgaagcgg gataggctga tgcccacccg ccacgaacag
     1021 cgatagatcg cagccgctgc tcaatagtgc gaccttcttc agcccggtcg ggcggctcgc
     1081 tgtatgggga cacgaaggaa cattcacgct tggcggtgat ggcgcacacc cacacatcct
     1141 cacgaaaccc ccaagcaata cccagggacg agccgggaca actaccggtg gggaacaggc
     1201 aaacgtccat ctcagtcagc gatcctggca ccggagaaca ccgccaggaa cagtgctcga
     1261 atgactcata gcccagcggg aagctggctg ccgcgccgcc ttcgcggctt gcccacgagg
     1321 cagtggtcac cgcgggcggg cctcacctag cagtcgagcc ggagccgtcg tcgccaggtg
     1381 cggtgtagtc ctttcgccgg ccgtgccacc cgcgtgccat aagcagcggt caccagcgga
     1441 atctcacgga cagccacggt cactccgaga aggccgttgc gctcgacgcc gaagccttac
     1501 tcggagtcgg gagcaaaccg aaactgcata gcatgcaagg ggtcaatgct ctggagcatt
     1561 gtgttagata tcactcgccg gctgtgtcaa gcagcacagc actgatcggg ccaacccatc
     1621 accctgaaga ggttcgacat ccaggagatg cgggagcgta ccgatggccc ccggtggcga
     1681 gcggctcgat gaccgctcag cggtcctgcc cgccgagcag cccgactctc gctcgacctg
     1741 gacc
//
LOCUS       DATADN010000502         4519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1254502, whole genome shotgun sequence.
ACCESSION   DATADN010000502 DATADN010000000
VERSION     DATADN010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254502"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VHH53_06550"
     CDS             complement(<1..186)
                     /locus_tag="VHH53_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289842.1"
                     /translation="MTTQASTVRELPDALRDGEEFGARLAGRRPAMFLDYDGVLTPIV
                     DRPEDAVMSDDMREAVQA"
     gene            395..1723
                     /locus_tag="VHH53_06555"
     CDS             395..1723
                     /locus_tag="VHH53_06555"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX2289843.1"
                     /translation="MGPTLINGAALAICETLGIGCWGCAVGGWFAGKLADRLFAAVLS
                     CSAAPRPRDPGHDWWCQVPGLAGMYWAWATRIRAGVIASVSMRHAVSYAILSASSPSA
                     SCACAAALGPGVMCMFIVVGEALVDLVGQRGGRTFVAHPGGSPANVALGLARLGVPVT
                     LMTRLGHDGLGKMIFEHLRASGVRVDGGPAQGTSTSLAVATLAAGVASYDFRIEWDMP
                     ALAPLPIETRCLHTGSLATALAPGNANVIELVERERTRGRVTISYDPNVRPALLSDTV
                     RARTDIERLVALSDVVKVSDEDLRWLYPDQPDELVAQDWLGLGPALVVVTRGGVGVYA
                     VSAGVELHRPVVPIDLVDTVGAGDSFTSGLLEALHRTDLIGGSRREAVAAIDESTLAS
                     VVEAAALTAAITCSRPGANPPTRAEIDALLAARDCSSDATSAGRGHLPTV"
     gene            1789..3150
                     /gene="fucP"
                     /locus_tag="VHH53_06560"
     CDS             1789..3150
                     /gene="fucP"
                     /locus_tag="VHH53_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HEX2289844.1"
                     /translation="MSAIEQPAAASPATAQPDRTTHPLVDKNMIVLFAVVICCFTAWG
                     IAADLTAPMVAAFKRIFTMSTFQASLVQFAYYGAYFALALPAAFINQRFGYKIGLLTG
                     LGLAALGALLFYPASEIMTYSMFLAALFTLAAGLSILETSANPYVMSMGPEANSTRRL
                     NLAQAFNPVGTNIGVLLATVLVLPKLNSPIDRASVTPEQLRAIQAEELKAVMVPYIGL
                     AILLLVIWVAIAVQKAPTIREEFPPASPSVGSRGEIIGQLWGNRRYRFGVIAQFFNIG
                     AQVCTWTFTIQYVEQALNGSLELGGFVLQISLLVFLGSRFLMTWLMGYFRATALMAIL
                     GGLAVVLCIYAMFSPNLSGVIAVAALSFCLSLMFPTIYGVALRGLGPATKFGAAGLVM
                     AIVGGALMPLVQGKVIDATSPAFSFIVPAVCYAVVMAYALYDLKATRPFEHDAPEPVP
                     AHT"
     gene            3253..>4519
                     /locus_tag="VHH53_06565"
     CDS             3253..>4519
                     /locus_tag="VHH53_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2289845.1"
                     /translation="MTRNRPSGLAALAAGLALLLAGCGGPPSTATSGPTRNLEVLSWW
                     TSGSERAALNVLFDDFRTAQPGVDVRNAAVTGGGGGNAHVVLASRLLGGDPPDVWQTH
                     PGASIKQYIDAGQIADVSSIYQRDNLSSAIRQDLRQAVSKDGKEYGVSTGAHRINMLW
                     FNKRLLDKAAVTVPAGGYTADAFLTDLAKLKAAGVVPLCLGAKDPFARAELFENTLLG
                     VIGADGWASLTSDRLNWNGPQVRQALSRFGTMLDYADPEAGALTWDQATRKLAAGECA
                     FPSMNDSAYGELVADGAKEGADFDYVPYPGTDNLFLTVVDTFVMAKNAKNPQNAQEFL
                     ATIGTAPTQLAFNKQKGSTPVRTDVDLTSLSPYQQSAAKAFAQHPVLLSITHGEAKSP
                     QFQQGFYDAIAAFAQSKDINVFNQALRNAV"
BASE COUNT          747 a         1461 c         1481 g          830 t
ORIGIN      
        1 ggcctggacc gcttcgcgca tgtcgtcgga catgactgcg tcctctggcc gatcgacgat
       61 cggggtcaac acgccgtcgt agtcgaggaa catggccggt cggcgaccgg ccagcctcgc
      121 gccgaactcc tcaccgtcgc gcagcgcgtc gggcagctcg cgaaccgtcg acgcctgcgt
      181 cgtcatggtc gtcggcgcgg cggcgtatgt gcaatcccgg ccggcatacc gaacctactt
      241 tgcgtggaga gttacctacc tctgtaagcg ggaatccgct gtggggcagg tcacgacgct
      301 aacacgtgct tgggtttagg tcttgccaag ttctgtacga caccggacgg tcggtcctgc
      361 tagcagcggt agccgccttc gggggctttc tgttttgggt ccgacgctga tcaacggggc
      421 agcgttggcc atttgtgaga cattggggat cggctgctgg ggttgcgcag tgggcggctg
      481 gttcgctggc aagctggccg accggttgtt cgccgcggtg ctgtcctgct cagcggcacc
      541 gcgcccgcgg gatccgggac acgactggtg gtgtcaagtg cccggtttag cgggtatgta
      601 ctgggcatgg gcgactcgga tacgcgcggg cgtgatagcc agcgtgagta tgagacacgc
      661 cgtcagctat gcgattctct ccgccagctc gccgagcgcg agttgtgcct gcgccgccgc
      721 gctcggtcca ggggtgatgt gcatgtttat tgtcgtcggg gaggcgcttg tcgacctggt
      781 cggccagcgc ggaggtcgga cgttcgtcgc ccatcctggc ggtagcccgg ctaacgtcgc
      841 gctcggtctg gcgcggctgg gtgttccggt tacgctcatg acgcgcctcg ggcacgacgg
      901 gctcggtaag atgatcttcg agcatctacg ggccagcggc gtccgtgttg acggaggtcc
      961 tgcacagggc acatcgacca gtctcgccgt tgcgacactg gccgcgggcg tcgcgtcgta
     1021 cgacttccgg atcgaatggg acatgccggc cctcgcgccc ctgccgatcg agacccgctg
     1081 cctgcacacc ggttcgctcg cgacggcgct ggcgcccggc aacgcgaacg tcatcgagct
     1141 ggtcgagcgg gagcgtacga ggggtcgggt gacgatctcc tatgacccga atgtacggcc
     1201 ggcactgctg agtgacacgg tgcgggcgcg gacggatatc gagcgtctgg ttgccctttc
     1261 cgacgttgtc aaggtcagcg acgaagacct tcggtggctc tatcccgacc agcccgatga
     1321 gctcgtggcc caggactggc tggggttagg accggcgctt gtggtggtga cccggggtgg
     1381 ggtgggggtt tacgccgtct ccgccggcgt tgagttgcac cgaccggtcg tgcctattga
     1441 tctggtggat accgtcggcg cgggtgattc cttcacctcc gggcttctcg aggccctgca
     1501 ccggaccgac ctgatcggcg ggtcgcgccg cgaagcagta gcggcgatcg acgagtcgac
     1561 cttggccagc gtcgtggagg cggcggccct gaccgcggcg atcacatgtt cccggccggg
     1621 tgccaacccg ccgacacgag ccgagatcga cgcgctgctc gcggcccggg attgctccag
     1681 cgacgcgacg agcgctggac gtggccactt gccgaccgta tgaggtcacc actgacgtcc
     1741 ggccacggga tgtgacgctt gcacgtcggg cggcgaaggg agaccgcaat gagcgcaatc
     1801 gagcagccgg cggccgcgag cccggcgaca gctcagccgg accgaaccac ccacccgttg
     1861 gtcgacaaga acatgatcgt gctgttcgcc gtcgtgattt gctgcttcac cgcatgggga
     1921 atcgccgcgg acctgaccgc tcccatggtg gcggctttca agcggatctt cacgatgagc
     1981 acgttccagg cttcgctggt gcagtttgcc tactacgggg cctacttcgc gctcgccctt
     2041 ccggcggcgt tcatcaacca gcggttcggc tacaaaatcg ggctgttgac cggactcggc
     2101 ctcgcggcgc tgggagccct cttgttttat ccggccagcg agatcatgac ctactccatg
     2161 ttcctggcgg cgctgtttac gctcgccgcc gggttgtcga tactggagac gtcggcaaat
     2221 ccttatgtca tgtcgatggg tccggaagcc aacagcacgc gacggctgaa cctggcgcag
     2281 gcgttcaacc cggtcggcac caatatcggg gtcttactcg ccaccgtact cgtcctaccc
     2341 aagctgaatt cgccaatcga ccgtgcctcg gtgacgcccg agcagttgcg cgctatccag
     2401 gccgaggagc tgaaggcggt catggtgccc tacatcgggc tggccatcct cctgttggtg
     2461 atttgggtcg ccatcgcggt ccaaaaggct ccgacgatcc gggaagaatt tccgccggca
     2521 agccccagcg tagggtcgcg cggcgagatc attgggcaac tctggggaaa ccgacgttat
     2581 cggttcggcg tcatcgcgca gttcttcaac atcggcgccc aggtgtgcac ctggacgttc
     2641 acgatccagt atgtcgagca ggccctcaac gggagcctgg agctgggcgg tttcgtactg
     2701 cagatcagcc tgcttgtttt tctcggctcc cggtttctga tgacctggtt gatggggtat
     2761 ttccgggcca ccgcgctcat ggcaatactg ggcggcctgg cagtggtgct gtgcatctat
     2821 gccatgttca gtccaaacct cagtggcgtc atcgcggtgg ccgcgctgtc gttttgcctg
     2881 tcgctgatgt tccccaccat ctacggcgtc gcgttgcggg ggctggggcc agcaacaaag
     2941 ttcggcgcgg ccggtctggt aatggcaatc gtcggcggcg cactgatgcc actggtccaa
     3001 gggaaggtca tcgacgcgac gagtccggca ttttccttca tcgtgccggc cgtctgctat
     3061 gcggtggtga tggcctacgc gctttacgac ctcaaggcga cacgaccgtt cgagcacgac
     3121 gcaccggagc ccgtgcctgc tcatacctga ccagccgtca ggcccggacg ccagcgcggc
     3181 cgccaaggcg gtaaggcgac ccgggtgaag gagcccgatc atcgctatcc cgccgtccta
     3241 ggagaatcga ccgtgactcg caatcgcccc tccggcctgg cggcgctggc tgccggcctc
     3301 gcgctcctac tcgccggctg cggcgggcca ccctcaacgg ctaccagcgg ccccacccgg
     3361 aacctcgagg ttctgtcctg gtggacgtca gggtcggagc gggccgcgct caacgtactg
     3421 ttcgacgact tccgaaccgc ccagcccggc gtagatgtcc gcaacgcggc ggttaccggc
     3481 ggcggaggcg gcaatgcaca tgtcgtcctg gccagccgcc tgctcggtgg tgatccaccg
     3541 gatgtgtggc agacccaccc cggcgcctcc atcaagcagt acatcgacgc ggggcagatc
     3601 gcggacgtga gctcgatcta ccaacgcgac aacctgtcat cggccattcg ccaagacttg
     3661 cgtcaagccg tctccaagga cgggaaggag tacggcgtct ccaccggagc gcaccggatc
     3721 aacatgctct ggttcaacaa gaggctgctg gacaaggcag cggtcacggt gccggccggc
     3781 ggctacacag ccgatgcgtt tctcaccgac ctggccaagc tgaaggcggc aggggtggtc
     3841 ccgctgtgcc tgggcgcgaa ggaccccttt gcccgcgccg aactgttcga gaacacgctg
     3901 ctgggtgtca tcggcgccga cggatgggca agcctgacat ccgaccggct gaactggaac
     3961 ggcccgcagg tccgccaggc cttgagtcgg ttcggcacga tgctggacta tgccgatccg
     4021 gaagccggcg cgttgacctg ggaccaggcg accagaaaac tcgccgccgg cgaatgtgcc
     4081 tttccgagca tgaacgactc ggcgtatggc gagctcgtcg cggacggcgc caaggaaggg
     4141 gccgacttcg attacgtgcc ctatcccggc accgacaacc tattcctgac cgtcgtcgac
     4201 accttcgtca tggcgaagaa cgccaagaac ccgcagaacg cccaggaatt cctggcaacg
     4261 atcggaaccg cccccaccca gctggccttc aacaaacaga agggttccac gccagtacga
     4321 accgacgtgg acctgacatc gttgtcgccc taccagcagt ccgcggcgaa agccttcgcg
     4381 caacaccctg tcctgctgtc gatcacgcac ggtgaagcaa aaagcccaca gttccagcag
     4441 ggtttctacg acgcaatcgc ggcgttcgcc cagagcaagg acatcaacgt cttcaaccag
     4501 gccctacgca acgccgtaa
//
LOCUS       DATADN010000503         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_45510, whole genome shotgun sequence.
ACCESSION   DATADN010000503 DATADN010000000
VERSION     DATADN010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45510"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VHH53_06570"
     CDS             <1..506
                     /locus_tag="VHH53_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2289846.1"
                     /translation="LPGLIAAGIDCIEHGTGLSADTITEMAARGTALVPTLINIETFP
                     AIADGAGKYPRYAAHMRALHAGLRDAVGSAVEAGIPVYAGSDAGGGIQHGRLIDEIAA
                     LRTVGLDGTRALAAASWAARDWLDAPGLDAGAPADLLVTRTDPRLDPNTLRDPALLLL
                     RGRVWGR"
     gene            582..1391
                     /locus_tag="VHH53_06575"
     CDS             582..1391
                     /locus_tag="VHH53_06575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2289847.1"
                     /translation="MSAPDPAQHPAVADTITPPEAVPGMRDRRRGWLLLGCFVIGEVT
                     FLTASLLVLVPYALASPRQSADGSLPPAALVVALVVPTVLAAMVATIGAALLGTGNLA
                     QRLRSQLSMRWRLSDVGVGLLLGVVGLIITVPAAGVWARWVGTNQANSAVGEAFEGLR
                     LPLGMALTLFFAVWLIAPLCEELLYRGVLWRAMEYWKWNRWVIFVVTTALFSVAHLEL
                     LRTPLLVVISLPIALARMLTGNLLASVVAHQANNFLPAVGLLLITQGLVPG"
BASE COUNT          189 a          504 c          539 g          284 t
ORIGIN      
        1 ctttgccggg gctgatcgcc gccgggatcg actgcatcga gcacggcacc ggcctgtctg
       61 ccgacaccat taccgagatg gccgcgcgcg gtaccgcgct ggttcccaca ctgatcaaca
      121 tcgagacgtt cccggcgatc gccgacggcg ccgggaagta tccgcgctac gccgcccaca
      181 tgcgcgcgct gcacgccggg ctccgcgacg ccgtcggttc ggccgtcgag gcggggatcc
      241 cggtgtacgc cggcagcgac gccggtggtg gcatccagca cggccgtctg atcgatgaga
      301 tcgccgcatt gcgcacggtg gggttggacg gcacgcgggc cctcgcggca gcgagctggg
      361 cggcccgcga ctggctggac gcgcctggcc tggatgccgg tgcgcccgcc gacctgctgg
      421 tcactcgcac cgatccgcgc ctggacccga acaccctgcg ggacccggcg ttgctgctgt
      481 tgcgtggccg ggtctgggga cgatgacaat gggcggacat caagcggggc ttgagcggac
      541 agtgggcggc agtggtccat ctccgatgac gaggagaaac cgtgagcgcg cctgatcctg
      601 cgcagcatcc tgcagtggcc gacacgatca cgccgccgga ggcggtgccg ggcatgcggg
      661 accgccgccg cggctggctg ctgctcggtt gcttcgtcat cggcgaggtg acgttcttga
      721 ccgcgtcgtt gctcgtgctg gtgccttatg cgctcgcttc cccgcgccaa tctgcggacg
      781 gttcgttgcc gcccgcggcg ctggtcgtcg cactggtagt gcccaccgtg ctggctgcca
      841 tggtcgcgac gatcggcgcc gcgttgctcg gaacgggcaa cctggcgcaa cggctgcgta
      901 gccagctgtc gatgcggtgg cgactgtccg atgtcggcgt cgggctgctg ctaggtgtcg
      961 tcggcttgat catcaccgtt ccggccgctg gtgtctgggc gcgctgggtc ggcaccaacc
     1021 aggcgaactc agcggtgggg gaggcgttcg agggcctgcg gctgcccctg gggatggcgc
     1081 tcaccctgtt cttcgccgtc tggctgatcg cgccgttgtg cgaggagttg ctctaccgcg
     1141 gcgtgctgtg gcgcgccatg gagtactgga agtggaatcg ctgggtgatc ttcgtggtga
     1201 ccacggcgct gttctccgtt gctcacctgg aactgctgcg caccccgctg ctggtggtga
     1261 tctcgctgcc gatagcgctg gcccgcatgc tcaccggcaa tctgttggcc agcgttgtcg
     1321 cccaccaggc gaataacttc ctgcccgcgg tcgggctgct gctgatcact cagggactag
     1381 tgcccggctg accccgtccc ccttaccggc gtgtcgtgct ctcatgcccc ctatgtcgtg
     1441 ttcccgtgca caacgtgtgg gcaggtgcca cggatgccgg atggtctggc acaatgagat
     1501 gctgttgtca cggcgc
//
LOCUS       DATADN010000504         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_371279, whole genome shotgun sequence.
ACCESSION   DATADN010000504 DATADN010000000
VERSION     DATADN010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371279"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VHH53_06580"
     CDS             <1..1308
                     /locus_tag="VHH53_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX2289848.1"
                     /translation="TAALGIAGLVLILAQGGLTTRLSMIRPALRSGIALATVAVAVSI
                     VVTGTALHLLLGLDWRTALLWGAVLSSTDAAAVFSVLRTLKVMPRLAGALELESGIND
                     APVYIVVVLLAAGDPLRWWTPLLVGYELLAGAVVGAGLGIAGAWVLRRAALPATGLYP
                     LAAIAVCVLAFAAGQGLHASGFLATYVAGLVLGNARLPHRGDTLSFAEGLGWLGEISL
                     FVLLGLYVSPSRLPDAVIPALVVGAVLIVLARPLSVVLALAPLRVPWREQMFLSWAGL
                     RGVVPIVLALVALTMGFPGAQLLADVVFVLVVLLTVVQGVTLPVVVRWLRLSQPEQGS
                     EIQVDFAPLDQMDAELLQIRVPRPSRLHGVYLSELRLPPGTMVSLVVRDGVVFTPEPT
                     TRLEEGDQLLVVTTAGNRDVAERRIRAVHRAGRLAAWRGETGA"
     gene            1342..>1690
                     /locus_tag="VHH53_06585"
     CDS             1342..>1690
                     /locus_tag="VHH53_06585"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX2289849.1"
                     /translation="MNPSQHGEAAAAGDQHIPDVPPGRRRTGLLAATAAGALLLDVLS
                     KIAVVAVLEGRPPVELLGGGLYLIVYRNPGAAFSMATGFTWLLSLIALGVVVAIVRLA
                     PRLRSTGWALGLGL"
BASE COUNT          181 a          532 c          647 g          330 t
ORIGIN      
        1 accgccgcgc tgggcatcgc gggcttggta ttgatcctgg cccagggtgg cctcaccacc
       61 cgattgtcga tgatccgtcc ggcgttacgt tcggggattg cgctggccac tgtggctgtc
      121 gcggtgagca tcgtcgtcac tggtacggca ctgcacttgc tgctgggcct ggactggcgc
      181 acggcgctgc tgtggggcgc ggtgctgtcc tcgaccgatg cggcagcggt cttcagcgtg
      241 ctgcggaccc tgaaggtgat gccccggtta gcgggtgcgc tcgagttgga gtcgggcatc
      301 aacgacgcgc cggtctacat cgtggtggtg ctactcgcgg cgggggatcc gctgcgttgg
      361 tggacgcctc tgctcgttgg ttacgagttg ctcgcaggcg cggtggtggg ggctgggctc
      421 ggtattgcgg gagcgtgggt gctgcgccgc gcggcgttac ctgcgaccgg tctgtacccg
      481 ctggcggcca tcgcggtctg cgtgctggcc ttcgccgccg ggcagggtct gcacgcctcg
      541 ggcttcctgg cgacctacgt ggccgggctg gtactgggca acgctcgcct accgcaccgc
      601 ggcgacacct tgtcgttcgc tgaagggctc ggctggctcg gcgagatcag cctgttcgtg
      661 ctgctgggcc tctacgtctc gccgtctcgg ttgcctgatg cggtgattcc cgcgctggtc
      721 gtcggcgcgg tgctgatcgt gctggcgcgc ccgttgtcag tggtgctcgc cttagcaccg
      781 ttgcgggtgc cctggcggga gcagatgttt ctgtcatggg cggggctgcg aggcgtggta
      841 ccgatcgtgc tggcgctggt tgcgctcacc atgggcttcc cgggcgcgca gctgctggcg
      901 gacgtggtct tcgttctggt ggtgctgctg acggtggtgc agggtgtcac actgcccgtg
      961 gtcgtccggt ggctgcggct gtcccagccg gagcagggca gcgaaatcca ggtcgacttc
     1021 gctccgctgg accagatgga cgccgaatta ctgcagatcc gagtcccccg cccctcgcgg
     1081 ctgcatggcg tgtacctctc ggagctgcgg ctgccccccg ggacgatggt cagcctggtg
     1141 gtgcgcgacg gcgtggtgtt caccccggag ccgaccaccc ggctggagga gggagatcag
     1201 ctgctggtcg tcaccacggc cggaaaccgg gatgtggccg agcgacggat ccgggcagtg
     1261 caccgggctg gccggctggc tgcgtggcgg ggggagaccg gcgcctgatc ggccgccgac
     1321 ggacgggtca caatgggcca tgtgaatcca tctcagcacg gcgaggcagc ggccgccggt
     1381 gaccagcaca tccccgacgt gccgccagga cgccgccgga ccggtctgct ggccgccacc
     1441 gcggccggcg cgctgttact ggacgtgctg agcaagatcg ccgtggtggc cgtcctggaa
     1501 ggtcgtccac cggtggagct gttgggcggc ggcctgtacc tcatcgtcta ccgcaacccg
     1561 ggcgcggcgt tctcgatggc gaccgggttc acctggctgc tgtcgctgat cgcgctgggg
     1621 gttgtggtgg cgatcgtccg cttggcaccc cggctgcgtt cgaccggctg ggcgttgggg
     1681 ctcgggctgg
//
LOCUS       DATADN010000505         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_517621, whole genome shotgun sequence.
ACCESSION   DATADN010000505 DATADN010000000
VERSION     DATADN010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517621"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1794)
                     /locus_tag="VHH53_06590"
     CDS             complement(64..1794)
                     /locus_tag="VHH53_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289850.1"
                     /translation="MKSRVAGAVPKASADSSLPAQPSAQLHPTEAGNTLARLLVKHIN
                     GSTEQYRRSQNAAQQRLNAFGSPHSSTTLHVEGALHLARPAFLPPDEEGYARLRHLDT
                     SLRMIEAAFPQVSTDIEVCRLTCTMLEGAWRSRALTPPREEEQTSVCACPLCRRLPTP
                     PARTLDDYRWRQSAGRPLTVKTWPHRRELDSVLTGGWSWTRQMNDLERDLGHRHRFED
                     DILVFHEYKRVIDTIDTTRLTQHVEGRLAENFSTNSARALVSGLFAAHKRSVDEYWLQ
                     QHDAGSVMLPQTNPLNQSTYSALQMLDCLFWHVAPDTELWQEENLASLLLCRVIDDMA
                     DVRADALTGEISNFWLADMPVHTKALYAACAIALVKYGCMPEAHSPMWNTWLMTTTIV
                     WMGLTGRHALWFDGITQGLPPAEECPLCDLQPNPCTGILTHGTTLTIAPRPTAPALST
                     RTAELSARCRAQYPRTWPLFDAELHAFEALHGPWYGNTDSTWEILRRTYIAAVEASSA
                     APSPDRARQVQQDAGVVGSERFHTLHDPQTGREDTALLAYMFGCAHPHFLWNATGYRP
                     TGVAGDWLDG"
     gene            2461..>2737
                     /locus_tag="VHH53_06595"
     CDS             2461..>2737
                     /locus_tag="VHH53_06595"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2289851.1"
                     /translation="MTTSAPARSAEQRKKDTLHRLEHDVDAWVATADGTSGTPYLVPL
                     SFLWNGSYLLFATPASSPTGRNLAATGRARVGIGPTRDVVLVEGTVET"
BASE COUNT          404 a          841 c         1003 g          489 t
ORIGIN      
        1 agccgacggc caccttcggg aggggccctt cggggcgcgg cccgagcctt cagggtcgat
       61 gcgtcagccg tcgagccagt cgcctgcgac gcccgtggga cggtatcccg tggcgttcca
      121 gaggaaatgc gggtgggcac agccgaacat gtaggcgagc agtgcggtgt cttccctccc
      181 ggtctgaggg tcgtgcaggg tgtggaacct ctcggatccg actacgccgg cgtcttgttg
      241 gacctgacgg gcgcggtccg gcgacggtgc ggcgctggat gcctccacgg cggcgatgta
      301 ggtgcggcgc aagatctccc atgtgctgtc cgtgttgccg taccacgggc cgtgcagtgc
      361 ttcgaaggca tggagctcgg cgtcgaagag gggccatgtc cgggggtatt gggctcgaca
      421 gcgtgcggac agctcggcgg tccgggtgct cagtgccggt gcggtgggcc gcggggcgat
      481 ggtgagggtg gtgccgtggg tgaggatgcc ggtgcacggg ttgggctgca ggtcgcacag
      541 cgggcactcc tcggccgggg ggaggccctg ggtgatgccg tcgaaccaca gggcgtggcg
      601 gcccgtgagc cccatccaga cgatggtggt ggtcatcagc caggtgttcc acatcgggct
      661 gtgggcctcg ggcatgcagc cgtacttgac gagggctatc gcgcaggcgg cgtagagcgc
      721 cttggtgtgg acgggcatgt cggcgagcca gaagttgctg atctcgccgg tgagggcgtc
      781 ggctcgtaca tcggccatgt cgtcgatgac gcggcacagc agcagggagg cgaggttttc
      841 ttcctgccag agttcggtgt cgggggcgac gtgccagaac aggcagtcca gcatctgcag
      901 ggctgagtag gtgctctggt tgagcgggtt ggtctggggg agcatgacgc tgcccgcgtc
      961 gtgctgttgg agccagtact cgtcgacgga tcgcttgtgg gctgcgaaga gtccggagac
     1021 cagggcccgt gcgctgttgg tgctgaaatt ctcggccaga cggccttcga cgtgttgggt
     1081 gaggcgcgtt gtgtcgatgg tgtcgatcac gcgtttgtat tcgtggaaga cgaggatgtc
     1141 gtcctcgaag cggtggcggt ggccgaggtc tcgttcgagg tcgttcatct gccgggtcca
     1201 ggaccatcca ccggtcagga cgctgtcgag ttcccggcgg tgtggccagg tcttgacggt
     1261 gaggggacga ccggcgctct gccgccagcg gtagtcgtcc agcgtgcgtg cggggggcgt
     1321 gggcagccga cggcacagag ggcaggcgca gacgcttgtc tgctcctcct cgcgcggcgg
     1381 ggtgagtgcc cggctcctcc aggccccttc cagcatcgtg caggtcagtc tgcagacctc
     1441 gatgtcggtg ctgacttggg ggaaggctgc ttcgatcatg cgcaggctgg tgtcgaggtg
     1501 cctcaacctg gcgtagccct cctcgtcggg cgggaggaac gcgggccgcg ccagatggag
     1561 ggctccctcc acatgcagcg tggtgctgga gtggggggag ccgaatgcgt tcagccgctg
     1621 ctgagcggcg ttctgggacc ttcggtactg ctccgtgctc ccattgatgt gtttcaccaa
     1681 gaggcgggcc agggtgttcc ccgcctccgt ggggtggagc tgggcgcttg gctgcgccgg
     1741 gagcgagctg tcggcggacg ccttcggaac agcacctgcg actcgagatt tcacaccggt
     1801 ctcccaatga gctgtggtcc tggcaacgga atcgatcgac cgtggtcgtt gaccggccgt
     1861 gtccgggaat cttctgtact ttccgcgacg gcctcgccgg acagccggac tcacgcgacc
     1921 ggctttggct tctgctacga acgaccgtga ggggcacgac tgctgcgatg acggcactcc
     1981 tgctgcaagg cgagcacctg catcgccgcc tcagctgtgt tgatcaggaa gcactcgcgg
     2041 cccgtcagac gtagcgcgac ccctggatcc gagatgagct gcttcgcccg aggggtgtgg
     2101 gtagctgacc ggggtgtcga agggcaggcc gaaccggacg tcctgaggca tgcgtttcct
     2161 ttcgggagac ggaacgcaga gtccccccac ccctgccggc ggacggtgcc gtgaccgccc
     2221 gcacactcga actgggtttc ccggtccgaa agttgaaccg agaagcggta cgagactgcc
     2281 caaggcgtga atcagccatt ccgtgaccct gccgtgatct ccgttactgc ccgaaccgca
     2341 tgagctgcgt atactccctc gtccctcacc gggtaccggc tcgtccaccg agcctccgga
     2401 gccgcggaac gccacactgg tcaaagtccg ttcgacgacc gaccaggagg cgcaccgacg
     2461 atgacgacat ccgcacccgc acgcagcgct gagcagcgca agaaggacac cctgcaccgc
     2521 ctggagcacg acgtggacgc ctgggtcgcc acggccgacg gcacgtccgg gacgccgtac
     2581 ctcgtcccac tgtccttcct ctggaacggc tcgtacctgc tgttcgccac ccccgcgtcg
     2641 agccccaccg gacggaatct ggccgcgacc ggccgggccc gcgtcggaat cgggccgacc
     2701 cgtgacgtag tgctcgtcga gggcaccgtg gagaccg
//
LOCUS       DATADN010000506         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1255205, whole genome shotgun sequence.
ACCESSION   DATADN010000506 DATADN010000000
VERSION     DATADN010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255205"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VHH53_06600"
     CDS             <1..558
                     /locus_tag="VHH53_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX2289852.1"
                     /translation="AEQVDPLSLARGYYLEPEAAGRKPYELLRAALQRTGRVGLAKIA
                     VRTKESLAVLRPRDDVLTLQTMMWPDEVREAQFDVLDRAVTVSDAEQAMADTLIEAMS
                     TDFEPGAYHDDYREALLAIVEAKSNGHDVAAPSTPAEPVPSVDLITALQASVEAAKAA
                     RGQPVDVPKQRKGDNGRRASKKSRS"
     gene            571..>1663
                     /gene="ligD"
                     /locus_tag="VHH53_06605"
     CDS             571..>1663
                     /gene="ligD"
                     /locus_tag="VHH53_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2289853.1"
                     /translation="MSDLVRPMLPTAGPLPAGPQWAFEFDWDGVRSLACSQPGRMRLF
                     SSTHRSISAAYPELEAFAGRRRMLLDGKIVALDSCGRPSFGQLQLRMNVQRPTSAMLR
                     RAPVAYYVFDVLRIDDRSTVGLPYQRRRELLEELDFAGGSVVLPPYFPDTDGQVVLDT
                     AAQYGLHGVIAKRADSSYQPGRSRSWVQTTLRQTQEVIIGGWVPERRRATEIGALLVG
                     VPTERGLRYAGQVGTGFTAARRRDLRELLPELEQQGSPFSDEVPQVAARAVHWVAPRL
                     LGEVSYRQWTPHGRLGHPTWRGLRPGKHVAAIRVPVVLSGREGGGEVTDQQMLADLER
                     AVQRLRAELRTLRDQISPHFLNNTLSTIAA"
BASE COUNT          263 a          566 c          576 g          258 t
ORIGIN      
        1 gccgagcagg tcgacccgct gtccctggcc cgcggctact acctggaacc cgaggcggcg
       61 ggccgcaagc cttacgagtt gctgcgcgct gcgctgcagc gcaccgggcg ggtgggactg
      121 gccaagatcg cggtgcggac caaggaatcg ctggccgtgc tgcggccccg cgacgacgtg
      181 ctcacgctac agaccatgat gtggccggat gaggtacggg aagcccagtt cgacgtgctc
      241 gaccgcgcag tgacggtcag cgacgccgag caggccatgg ccgacaccct catcgaggcg
      301 atgtccaccg acttcgaacc cggcgcctac cacgatgatt accgcgaggc cctgctcgct
      361 atcgtcgaag ccaagagcaa cgggcacgac gtcgccgccc cctcgacgcc ggccgagccg
      421 gtgccctcag tggacctgat caccgcgttg caggcctcgg tcgaggcggc caaggccgct
      481 cggggacagc cggtggatgt gccgaaacag cgcaagggcg ataacgggcg gcgggcgtcg
      541 aagaagtccc gctcctgatc gggaggagag atgtccgacc tggtccgccc catgctgccg
      601 accgcggggc cgctgcctgc cgggccgcaa tgggcatttg agttcgactg ggacggggtg
      661 cgctcgctgg cctgctccca gcccggccgg atgcgcctgt tcagcagcac ccatcgcagc
      721 atctccgccg cctacccgga actcgaagcg ttcgccggac ggcgccgcat gctgctggac
      781 ggcaagatcg ttgccctgga ttcctgcggc cggcccagct tcgggcagct ccagttgcgg
      841 atgaacgtgc aacgtccgac gtcggcgatg ctgcgccggg cgccggttgc ctactacgtg
      901 ttcgacgtgc tgcgcatcga tgatcgatcc accgtcgggc tgccctacca gcgccggcgt
      961 gagctgctgg aagagctgga tttcgccggg ggctcggtcg tgctgccgcc gtactttccc
     1021 gataccgacg gccaggtcgt gctggacacc gctgcgcagt acggcctgca cggtgtgatc
     1081 gccaagcgcg ctgactccag ttaccaaccc ggccggtccc gtagctgggt gcaaaccacg
     1141 ctgcggcaga cccaggaagt gatcatcggt ggctgggttc ccgagcgacg ccgcgctacc
     1201 gagatcggtg ccctgctcgt gggggtgccc accgagcgcg gcctgcggta cgccggccaa
     1261 gtggggacgg gtttcaccgc cgctagacgg cgggacttgc gcgagttgtt acccgagctg
     1321 gaacagcagg gcagtccatt cagcgatgag gtgccgcaag tcgccgctcg ggccgtgcac
     1381 tgggtggcgc cgcgactgct cggcgaggtc tcctaccggc aatggacacc gcatggccgc
     1441 ctcgggcatc ccacctggcg cggtctgcga cccggcaagc atgtggcggc tatccgggtg
     1501 ccggtcgtgc tcagtgggcg agagggcgga ggcgaggtga ccgaccagca aatgctcgcc
     1561 gacctagaac gcgccgtgca gcgacttcgt gccgagttgc ggacgttgcg cgaccagatc
     1621 tccccgcact ttctcaacaa cacattgagc acgattgctg cct
//
LOCUS       DATADN010000507         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_371750, whole genome shotgun sequence.
ACCESSION   DATADN010000507 DATADN010000000
VERSION     DATADN010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371750"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VHH53_06610"
     CDS             <1..384
                     /locus_tag="VHH53_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2289854.1"
                     /translation="HLRKMVSSRPAAWASTEMTLAQLLALHFISAKAPITLLSLSEAL
                     GTRPSATSTMVDRLARAGLVSRTPDPDDRRRILLAVSGRAVAMIGKVDLQTARRLQVV
                     VNGMGTAAQRCLTEVLKDTARRLAG"
     gene            543..>1622
                     /locus_tag="VHH53_06615"
     CDS             543..>1622
                     /locus_tag="VHH53_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289855.1"
                     /translation="MTDSNGRNPADRQRLPVVIAGAGTPTGLAIARGLRDTGAPIVGA
                     SLTPTAPTCRSKLWHRVLPVSAPTTEAWLTTLRAAHARYGPMVLLPADDAVVRVVAEH
                     AGELATQFHFVLPDLPIVDRLLDKSVFHEWALANDFPVPHTAIVTNPDQLRAALREMT
                     FPVVLKPVEHTPQWAAVSRHYKVHRFHSITDLERLPFAPFDAADRFVVQEWIPGRDSD
                     VYFCLTYRNRNGEELAAQGGRKITQWRVDTGSTALAVTHHDAELHKLTRRLLDTAGHV
                     GFGSLEIRRSTRDGRMIITEPTVGRPDLQTALAAAAGVNLVDMAYRDALRLPALPSRP
                     RREAIWIHETAFPRSVLVAARRRRLD"
BASE COUNT          272 a          579 c          508 g          263 t
ORIGIN      
        1 cacctacgga agatggtgtc gtcccgcccc gccgcctggg cgagcaccga gatgaccctg
       61 gcccagttgc tcgcgctgca cttcatcagc gcaaaggctc cgatcacact gctctcgctc
      121 tctgaggcct tgggcacccg accgtccgcc acctccacaa tggttgatcg gctcgcccgg
      181 gctgggctgg tctctcgcac gccagacccc gacgaccgcc ggcgcatcct gctggccgtg
      241 agcggtaggg cggtagcgat gatcggtaag gtcgacctgc aaaccgctcg acggctgcag
      301 gtggtggtca acggcatggg cacggcagcc cagcgctgcc tcactgaggt cctcaaggac
      361 actgcacgcc gcctcgccgg ctaaccagac gatgccaagc ccgtcacgga gcctcaccga
      421 acgtcggttg gtcccgctaa ggtgccgtcc gcgcgagccg atcaagctca tgggttgaca
      481 aggtgcggcg gcgaagctgg ccgtcgggct gcctcgttgc gccataagct agcgtcacac
      541 agatgactga cagtaacggt cgtaatcccg ccgaccggca gaggttgccg gtggtcatcg
      601 caggtgccgg aactcccact gggctggcca tcgcccgggg cctgcgcgat acgggagccc
      661 ccatcgttgg cgcctctctc accccgacgg cgccaacctg ccgctccaag ctatggcacc
      721 gcgtcttgcc agtcagcgca cccaccacgg aggcatggct gacaacgctg cgggcggctc
      781 acgcgcggta cggcccgatg gtgctgcttc cggccgacga cgcggtggtg cgcgtcgtcg
      841 ctgagcacgc cggcgaactg gccacgcagt tccacttcgt gctgcctgac ctgccgatcg
      901 tggatcgatt gctggacaag tcagttttcc acgagtgggc cctggccaac gacttcccgg
      961 tgccccacac agcgatcgtg accaaccctg accagttgcg cgcagccctg cgcgagatga
     1021 cctttcctgt ggtgctcaag ccggtcgagc acacgccaca atgggcggcc gtcagtaggc
     1081 actacaaggt ccaccgattc cactccatca ccgatcttga acggcttccg ttcgctccgt
     1141 tcgatgccgc ggaccgtttc gtcgtccagg agtggattcc gggccgagac agcgacgtct
     1201 acttctgcct gacctaccgc aaccgcaacg gcgaagagct agcagcccaa ggcgggcgaa
     1261 aaatcaccca gtggcgtgtc gacacgggca gcactgccct tgccgtgacc caccacgacg
     1321 cggagttgca caagctgacc cggcggctgc tcgacaccgc cggccacgtc ggcttcggct
     1381 cgttggagat tcggcgcagc acccgagatg gccggatgat catcaccgag ccgaccgtgg
     1441 gccgaccgga cttgcagacc gcactggccg ctgccgccgg ggtgaacctg gtggatatgg
     1501 cgtatcgcga cgcactgagg ctgcccgcgc taccgagtcg accccgccgg gaggcgatct
     1561 ggattcacga gaccgccttt ccgcggtcgg tgctcgtcgc ggcacgccgc cgccgcctcg
     1621 ac
//
LOCUS       DATADN010000508         4065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_202655, whole genome shotgun sequence.
ACCESSION   DATADN010000508 DATADN010000000
VERSION     DATADN010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202655"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VHH53_06620"
     CDS             complement(<1..311)
                     /locus_tag="VHH53_06620"
                     /inference="COORDINATES: protein motif:HMM:NF020858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEX2289856.1"
                     /translation="MTSWTWVAADHPLGLHTLPYGVFTTAVQSQPRIGVAVGDVILDL
                     TTATTAALPECAALFRSGSLDALLAAGRQTWTTVRDTLTRWLTDDRHAEALAPLLLPA
                     A"
     gene            complement(308..1507)
                     /locus_tag="VHH53_06625"
     CDS             complement(308..1507)
                     /locus_tag="VHH53_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX2289857.1"
                     /translation="MAYYRQAGTIPPKRHTQHRRPDGSLYYEELMGTDGFSSDSSLLY
                     HEEIPSAIVDARPWSLPDHSTTPNEPLLPRHLRLHDLFDSQEWKCHDVVTARRLVLGN
                     ADVRIYYVVAGKVSPLYRNAVGDECIYVESGSATVQTVFGPLEVGTGDYVVLPRSTTH
                     RWVPNPDGPLRAYCIEAGSHLAPPQRYLSRFGQFLEHAPYCERDLRGPGAPEVVESTD
                     VDVLVKHRGARGVTGTIYTYPRHPFDVVGWDGCLYPYAFNIADFEPITGRIHQPPPAH
                     QVFAGTGFVICNFVPRKVDYHPLAVPVPYYHSNVDSDEVMFYCGGDYEARKGSGIGPG
                     SVSLHPGGHSHGPQPGAIERSLGAESFDELAVMVDTFRPLERGEGGLACEDPAYPWSW
                     SGRGPAR"
     gene            complement(1512..2684)
                     /gene="hppD"
                     /locus_tag="VHH53_06630"
     CDS             complement(1512..2684)
                     /gene="hppD"
                     /locus_tag="VHH53_06630"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX2289858.1"
                     /translation="MSHTLSIAGASVDTDADALIGSVAHDVDPDPFPVIGMDHIRFAV
                     GNARQATHFYSTAFGMTCVAYRGPEQGYRDAAEYVLVSGRVRFVLTGAVRAGTPLAQW
                     VAAHGDGVSDLAIEVPDVDGAVAFARQQGATVLEEPADATDEHGTVRLAALATYGDTR
                     HTLVDRSGYTGQFLPGFVGQPPLVTPVRRLFQAVDHCVGNVELGRMDFWVSFYNRVMG
                     FENMKEFVGDDIATQYSALMSKVVASGNHRVKFPLNEPAAGRKRSQIDEYLQFYGGPG
                     VQHIALATGDIVSAVTAMRERGVEFLDTPDSYYDTLGEWVGDTRVPVQTLRELRILAD
                     RDEDGYLLQVFTKPVQDRPTVFFELIERHGSLGFGKGNFKALFEAIEREQARRGNL"
     gene            2877..3899
                     /locus_tag="VHH53_06635"
     CDS             2877..3899
                     /locus_tag="VHH53_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX2289859.1"
                     /translation="MRIGVLTGGGDCPGLNAVIRAVVRKGIEVYGHEIIGFRDGWRGP
                     MQGLTQPLGLADVEHILPLGGTILRTSRTNPYRNGGAEQVKQTLAEHRVDALIAIGGE
                     DTLGVARRLGEDGIFVVGVPKTIDNDLSATDYTFGFDTAVHIATEAIDRLRTTAESHH
                     RAAVVEVMGRNAGWIALHSGMAGGANAILMPEQPFSVDRVCQWVIRCLERDDAAIVVV
                     AEGAVPEEGTEMLTTAEVDAFGHKRLGGVGTWLADQIARCTGAESRAVVLGHIQRGGT
                     PTAYDRVLATRFGVHAIDAVHEDHGGVMVALRGTNIIRVKLAEATAAVKTVPQDRHAE
                     AEVFFG"
BASE COUNT          712 a         1318 c         1368 g          667 t
ORIGIN      
        1 tcggcagccg gtaggagcag cggcgccagc gcttcggcgt gccgatcgtc ggtcagccag
       61 cgggttaggg tgtcccgaac ggtggtccag gtctggcgtc ccgcggccag caacgcatcg
      121 agactgccgc tgcggaacag cgcagcgcac tcaggcaggg cagccgtggt agctgtagtg
      181 aggtcgagga tgacgtcgcc taccgctact ccgatccgcg gctgcgactg gaccgcggtg
      241 gtgaagacgc cgtacggcag cgtgtgcagg cccaacggat gatcggccgc cacccaggtc
      301 caactggtca cctggctgga ccccgtcccg accagctcca cgggtacgcc gggtcctcgc
      361 acgcgaggcc gccctcgccc cgctccagcg ggcggaaagt gtcgaccatg accgccagct
      421 cgtcgaagga ctcggcccca aggctgcgct cgatcgctcc cggctgcggg ccgtgcgagt
      481 ggccaccggg atgcagcgag accgagcccg ggccgattcc gctgcccttg cgcgcctcgt
      541 agtcgccacc gcagtagaac atgacctcgt cggagtcaac gttggagtgg tagtagggca
      601 ccggtaccgc tagcgggtgg tagtcgacct tgcgcggcac gaaattgcag atcacgaaac
      661 cggtgccggc aaagacctgg tgtgccggcg gtggctgatg gatgcgaccg gtgatcggct
      721 cgaagtcggc gatgttgaag gcgtagggat acaggcagcc gtcccagccc accacgtcga
      781 aggggtgtcg cgggtaggtg tagatggtgc cggtgacacc tcgagccccg cggtgcttga
      841 ccagaacatc cacgtcggtg ctctcaacca cctccggcgc accgggcccc cgcagatccc
      901 gctcgcagta cggcgcgtgc tccaggaact gcccgaaccg ggacaggtag cgctgcggcg
      961 gggcgaggtg gctgccggcc tcgatgcagt acgcacgcaa cggtccgtcc gggttcggca
     1021 cccaccggtg ggtggtgctg cgcggcagca cgacgtaatc gcccgtcccg acttcaagtg
     1081 gaccgaagac cgtctgcacc gttgcagacc cggactcgac atagatgcac tcgtcgccca
     1141 cagcgttgcg gtacagcggc gaaactttac ccgcgacgac gtagtagatc cgcacgtcgg
     1201 cattgccgag caccagccgc cgcgcagtga ccacgtcgtg gcatttccac tcctggctgt
     1261 cgaacaggtc gtgtaatcgc aagtgccgcg gtagcagtgg ctcgttgggt gtcgtgctgt
     1321 ggtcgggcag gctccacggc cgggcgtcga cgatcgccga ggggatctcc tcgtggtaaa
     1381 gcagcgagga gtccgaggag aaaccatcgg tgcccatcag ctcctcgtag tacagggagc
     1441 cgtcggggcg gcggtgctgg gtgtgccgct tgggtgggat cgtgcccgcc tgccggtagt
     1501 aagccatggc gctacaggtt gccgcggcgg gcctgctcgc gttcgatggc ttcgaacaag
     1561 gccttgaagt tgcccttgcc gaagcccagc gagccgtgcc gctcgatgag ttcgaagaac
     1621 accgtcggcc gatcctgcac cggtttggtg aacacctgca gcaggtagcc gtcttcatcc
     1681 cggtcggcaa ggatgcgtag ctcacgcaag gtctggacgg ggacgcgggt atcgccgacc
     1741 cactcgccga gggtgtcgta gtaggagtcc ggtgtgtcca ggaactccac gcccctttcc
     1801 cgcatcgccg tcacggcgct gacgatgtca ccggtcgcca gcgcaatgtg ctgcacgccg
     1861 gggccgccgt agaactgcag gtactcgtca atctgcgagc gcttgcggcc ggccgcgggt
     1921 tcgttgagcg ggaacttgac ccggtggtta ccactggcca ccaccttcga catcagcgcg
     1981 gaatactgag tggcgatgtc gtcaccgacg aactctttca tgttctcgaa gcccatgacc
     2041 cggttgtaga acgacaccca gaagtccatg cggccgagct cgacgttgcc cacgcagtga
     2101 tccacagcct ggaacagccg gcgtacgggg gtcaccagcg gtggctgtcc gacgaaaccc
     2161 ggcaggaact ggccggtgta gccggaccga tcgacgaggg tatgccgggt atcgccatag
     2221 gtagccagtg cggccaggcg tacggtgccg tgctcgtcgg tggcgtcggc cggttcctcg
     2281 aggacggtcg cgccctgctg gcgggcgaac gcgacggccc cgtccacgtc tggcacctcg
     2341 atggcgaggt cgctcacccc gtcaccgtgt gccgccaccc actgggccag tggcgtaccg
     2401 gcccgcacag caccggtcag cacgaatcgg acccgaccgg agaccagcac atactccgct
     2461 gcgtcccggt aaccctgctc tggaccccgg taggccacgc aagtcatgcc gaacgccgtc
     2521 gaatagaagt gtgtggcctg ccgtgcgtta ccgacggcaa accgtatgtg atccatcccg
     2581 atcactggga acgggtcagg atcgacatcg tgagcgacgc tacctatcaa ggcgtcggca
     2641 tcggtgtcca ccgatgctcc ggcgatgctc aatgtgtggc tcatagcgcg gacccctcgt
     2701 ctcagggtac gcgtcaatga ctccgcagcg ctgggccgcg tgaaccgagt gcggcccgat
     2761 cacgacgccg tcgcgggcga tgcgcgccca ggggggccca cttgcccttc tctcgagcac
     2821 cgtggagcct ggctgtgaac cctcaatccg ggtactaagc gacaaggcga atcgacatgc
     2881 gtatcggcgt gctcacgggg ggtggtgact gccccggact caatgcggtg atccgggcgg
     2941 tggtccgcaa gggaatcgaa gtgtacgggc acgagatcat cggattccgg gatggctggc
     3001 ggggtccgat gcaggggttg acgcagccac tcggtctggc tgacgtggag cacatcctcc
     3061 cgctcggcgg caccatactg cgcacatcac gcacgaaccc gtaccgcaac ggcggcgcgg
     3121 agcaagtcaa gcagaccctc gctgaacacc gggtggatgc gctgatcgcg atcggcggtg
     3181 aggacaccct cggggtggcc cgccggctgg gcgaggacgg gatcttcgtg gtgggtgtac
     3241 ccaagaccat cgacaacgac ctaagcgcca ccgactacac cttcgggttc gacaccgcgg
     3301 tgcacatcgc gaccgaggcg atcgaccggc tgcgtaccac cgcggagtcg caccatcggg
     3361 cggcggtcgt ggaggtgatg ggccgtaacg cgggctggat tgcgctgcac tccggaatgg
     3421 ctggcggggc gaacgccatt ctgatgccgg agcagccgtt ctccgtggat cgggtgtgcc
     3481 agtgggtcat ccggtgtctc gagcgcgacg acgccgcgat cgtcgtggtc gccgagggcg
     3541 cagtaccgga agaaggcacg gagatgctca ccaccgcaga agtcgacgcc ttcggccaca
     3601 agcgcctcgg gggtgtcggc acctggttgg ccgaccagat cgcccgttgc accggtgctg
     3661 agtcccgcgc tgtggtgctg gggcacatcc agcgcggtgg cacccccact gcctacgacc
     3721 gggtactggc cacccgattc ggcgtgcatg cgatcgatgc ggtgcatgaa gaccacggcg
     3781 gtgtgatggt cgcgctgcgc ggcacgaaca tcatccgagt gaagctggct gaggccaccg
     3841 cagcagtcaa aacggtgcca caggacagac acgcggaggc cgaagttttc ttcggttgac
     3901 catcagctca agacaatgcg caatcacctt ccgaattgaa gccctctcac catcaaattc
     3961 acatcccgcg ctaagaagtc ttcgccggat tctgcccttt cgttcgagat gctatccgta
     4021 cctgcgtcga atcgcgaacg ccccgaccag tgatcttcac aatgc
//
LOCUS       DATADN010000509         2244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_974515, whole genome shotgun sequence.
ACCESSION   DATADN010000509 DATADN010000000
VERSION     DATADN010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974515"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..479
                     /locus_tag="VHH53_06640"
     CDS             114..479
                     /locus_tag="VHH53_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HEX2289860.1"
                     /translation="MRSVRRCSKSGCTYPAVATLTFVYSDSTAVVGPLATCNEPHSYD
                     LCERHALRLTAPRGWDVVRHDGQFAELEPSVDDLTALAEAVREAGRADRPMDPVLAGP
                     ASGSGRRGHLWVLPDPVDS"
     gene            596..1987
                     /locus_tag="VHH53_06645"
     CDS             596..1987
                     /locus_tag="VHH53_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX2289861.1"
                     /translation="MPDLSTIVKAYDIRGVVGEQLDAAVVRDIGAAFAELVRDIEGSH
                     AVVVGYDMRESSPVLAGAFAEGVTGRGLDVVSIGLASTDMLYFASGRLGLPAAMFTAS
                     HNPARYNGIKLCRAGAAPVGQDSGLEEIRVAVERGIEPGPGGGRITEREMLSDYAEHL
                     RSLVDLSGIRPLRVVVDAGNGMGGHTVPAVVQGLPLHIIPLYFELDGNFPNHEANPLD
                     PANLVDLQTAVREHGADLGLAFDGDADRCFVIDELGEPVSPSAITALVATRQLAIEPG
                     ATVIYNLITSQAVPEIVAEHGGRPVRTRVGHSFIKQKMAETGAIFGGEHSAHYYFRGF
                     WRADSGMLAALHVLAALGHQPGPLSALMAEFSRYAASGEINSTVDDQQGRMAAVREWF
                     GRRDGVELDEVDGLTVRLPGGAWFNLRPSNTEPLLRLNVEARDTAAVEALRDEVLALV
                     RQVGSSSRITTGQ"
     gene            2036..>2244
                     /locus_tag="VHH53_06650"
     CDS             2036..>2244
                     /locus_tag="VHH53_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX2289862.1"
                     /translation="MAVDVDPQLMEILACPCPEHAALRSGTMADPEADALTCTACGRS
                     FPVIDGIPVLLLDEALPGTGPPAGAR"
BASE COUNT          349 a          712 c          797 g          386 t
ORIGIN      
        1 ccgggtccca gtcatccgtt gagcctacgc cgcagcgcac cggccgggat ggtcccgaga
       61 catcacagcg cgcctagcac aacgagctgg acctgcgcgc tatggtcggc gccgtgcgca
      121 gcgtgcggcg gtgctcgaag agcgggtgca catacccggc ggtcgccacg ctgacctttg
      181 tgtattcaga ttccactgct gtcgtgggcc cgctggccac ctgcaacgag ccgcattcct
      241 acgatctgtg tgagcggcat gcgctgcgcc tgactgctcc acgaggctgg gacgtcgtgc
      301 ggcatgacgg tcagttcgcc gagttggagc cgtcggtgga cgacctgacc gcgctggccg
      361 aggcggtacg ggaggccggc cgggcagacc gtccaatgga tcccgttctc gccggtccag
      421 cgagcggaag tggtcgccgg gggcacctgt gggtcctgcc ggacccagtc gacagctgag
      481 cccgtccccg tcgcctctag cgtcccggtg ggagggatcg ccgcgcagct aggctcggtg
      541 gcgccacctg gactcgacgc agggtccacg aagccggacc tgatgaggga gcgcagtgcc
      601 tgacctgtca acgatagtga aggcgtacga catccggggt gtcgtcggtg agcagttgga
      661 cgctgcggtg gtacgcgaca tcggtgccgc gttcgccgag ttggtgcgtg acatagaggg
      721 ctcgcatgca gtggtcgtcg ggtatgacat gcgggagtcc tcgcccgtac tggcgggcgc
      781 gtttgccgag ggagtgaccg gccgtggtct cgacgtcgtc tcaatcggac tagccagcac
      841 cgacatgctg tacttcgctt ccggccggct tggcttgccg gccgcgatgt tcaccgcaag
      901 ccacaacccg gcccgctaca acggcatcaa actctgccgg gccggcgctg cgccggttgg
      961 ccaggacagc gggctggagg agatccgggt cgcggttgag cgggggatcg aaccagggcc
     1021 cggaggcggt cggatcaccg agcgggagat gctcagtgac tacgctgagc acctgcgctc
     1081 gctggtggac ttgtccggaa tccggccgct ccgagtcgtc gtggacgccg gtaatggaat
     1141 gggggggcat accgtgcccg cggtggtgca gggactgccg ctgcacatca tcccgttgta
     1201 cttcgaactg gacggcaact tccccaacca cgaggccaac ccgctggacc cggcgaatct
     1261 ggtggaccta cagaccgcgg tccgcgagca cggtgccgat ctgggcctgg ccttcgatgg
     1321 cgatgccgac cgttgtttcg tgatcgacga gctcggtgag ccggtgagcc ccagcgccat
     1381 cactgccttg gttgcgacac ggcagctggc catcgagccg ggcgctacgg tgatctacaa
     1441 tttgatcact tcacaggccg tgccggagat cgtcgccgag cacggcgggc gcccggtacg
     1501 cacccgggtc ggccactcgt tcatcaagca gaagatggcc gagacggggg ccattttcgg
     1561 cggcgagcat tccgcgcact actacttccg gggcttctgg cgggccgact cggggatgct
     1621 ggcggcgctg cacgtgctgg cggcactggg ccatcaaccg ggtccgctct cggcgctgat
     1681 ggccgagttc agccgttacg ccgcgtccgg tgagatcaac tccacggtcg atgatcagca
     1741 gggcaggatg gcggcggtgc gggagtggtt cggtcggcgc gacggcgtcg agctcgatga
     1801 ggtcgacggc ctcaccgtgc gattgcctgg cggtgcatgg ttcaacctgc gcccgtcgaa
     1861 caccgagccg ctgctgcgcc tcaacgtcga ggcccgcgac acggccgcgg tagaagcgct
     1921 acgtgacgag gtgctcgcac tggtacgcca agttggtagc tcttcaagga ttaccaccgg
     1981 gcagtgaggt ggccttcaag agctaccaac ccgcccggta aggaggagga cgacgatggc
     2041 tgtggatgtg gatcctcagc tgatggagat tctggcctgc ccgtgtcccg aacatgccgc
     2101 gctgcgcagc ggcaccatgg ctgaccccga ggcggatgcg ttgacctgta ccgcgtgcgg
     2161 gcgcagcttt ccggtgatcg atggcatccc ggtgctgctg cttgatgagg cactgcccgg
     2221 cactggaccg cctgccggtg cccg
//
LOCUS       DATADN010000510         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1124601, whole genome shotgun sequence.
ACCESSION   DATADN010000510 DATADN010000000
VERSION     DATADN010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124601"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VHH53_06655"
     CDS             complement(<1..329)
                     /locus_tag="VHH53_06655"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2289863.1"
                     /translation="MAAPYELRAHRALETPVLLLATDSWLDAGFGAQGAIAAILETMS
                     TEVLATFTADEFIDFRARRPTAQIADGVLAGLNWPEIQLRSGQDGAGRSVLVLVGPEP
                     DMAWHAFV"
     gene            667..>1596
                     /locus_tag="VHH53_06660"
     CDS             667..>1596
                     /locus_tag="VHH53_06660"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX2289864.1"
                     /translation="MSALGMSAMRRIVHEVRSVEIGASTRYERYQLVVDRDQAEALFA
                     DPALASVRAHSAAPGEQVRIIAPLDVVEPRTKGPGGGVFPGWMAPVNRKRDGDTHVLR
                     GAAVLAAGYLPRNQEGLIDMSGPAAELSPFGRTNNVVIQFDRADGAPWQEVEVAVRRG
                     LLRLAVHLADAAVDAAPDAIEELAPPHQPGTPTGGLPRVGVITNLQTQGAFKDVFVYG
                     RSMAGSLPTLFDPAEIEDGAVVSGQYGHPALRNPTYLHQNHPVIAELRARDGQDLCFA
                     GVVLCPEPVEQKDKELVSTHAAWLCRATGFDAVI"
BASE COUNT          284 a          513 c          543 g          256 t
ORIGIN      
        1 acgaaagcgt gccacgccat gtccggctcg ggcccgacta agaccaagac actcctaccc
       61 gcaccgtcct gacctgaccg cagctggatc tcgggccagt tcaagccggc aaggacgccg
      121 tcagcaatct gggcggtggg acggcgagcc cggaaatcga tgaactcgtc ggcggtgaag
      181 gtggccagca cctcggtcga cattgtctcg agaatcgcag ctatggcacc ttgggccccg
      241 aacccggcat caagccagct gtcggtggcc agcagcagca ccggtgtctc cagtgcgcgg
      301 tgagcgcgta actcgtacgg agcggccatc atcggacggt ggggatcggt accgggatgc
      361 tcatgcgaca agtctcccgc tcaggcgagc tcagtggagt aagcaacaga gattccgacg
      421 ggcacgacca tcaccgctgg tctcggcggc gagtgcctga cgctcgtttt cgccgaaagc
      481 acggtgtttg cctgatggtc ggcgagccag gcgtcgacct ggcgagcgcg aagttacctg
      541 ataagcgttt cagtcaccac cctgcccgcg catgtccacg acgaaggtgt ggttgtccag
      601 cacatcgctg cgatgatcgg tgaatccgtg gttacgatgc cctcaagcca ccgaggggag
      661 accgtcgtgt cggcactggg catgtccgca atgcgacgaa tcgtgcatga ggtccgctcc
      721 gtcgagatcg gcgccagcac tcgatacgag cgatatcagc ttgtggtcga ccgggaccag
      781 gctgaggcgc tgttcgccga tcctgccctg gcgtcggtac gcgcacacag cgcggcgccg
      841 ggagaacagg ttcggatcat cgcgcccctg gacgtggtgg agccgcgtac gaaggggccg
      901 ggcggtggag tttttcccgg ctggatggct ccggtcaacc gcaagcggga cggcgacacg
      961 cacgtgctgc gtggagcggc ggtgctggcc gctggatacc tgccacgaaa ccaggaaggt
     1021 ctgatcgaca tgtccggtcc ggccgctgag ctgtcaccgt tcggtcggac gaacaacgtc
     1081 gtgatccaat tcgaccgcgc ggacggtgcc ccctggcagg aggtcgaggt cgcggtgcgc
     1141 cgcggcttgt tgcggttggc ggtgcacctg gcggatgccg cggtcgacgc cgcgcctgac
     1201 gcgatcgagg agttggcgcc accgcaccag cccggcacac cgacgggcgg gctgccacgc
     1261 gttggggtga tcaccaactt acagacgcag ggagcgttca aggacgtctt cgtctacggg
     1321 cgcagcatgg cgggcagcct gccgaccttg ttcgaccccg ccgagatcga ggacggcgca
     1381 gtggtcagcg gccagtacgg gcacccggcc ctgcgcaacc cgacttacct gcaccagaac
     1441 catccggtga tcgccgagct gcgcgcccga gacggccagg acctgtgctt cgccggcgtg
     1501 gtgctgtgcc cggagccagt ggagcaaaaa gacaaagaac tcgtgtccac acacgccgcc
     1561 tggctgtgcc gcgccaccgg cttcgacgct gtcatc
//
LOCUS       DATADN010000511         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_46495, whole genome shotgun sequence.
ACCESSION   DATADN010000511 DATADN010000000
VERSION     DATADN010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46495"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VHH53_06665"
     CDS             <1..1151
                     /locus_tag="VHH53_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289865.1"
                     /translation="RALAEAAWPTERKVCAIITRETDEGGIENVRVLREEYGDAFAEF
                     IHILDPLEPGIVVGKSSAMAWGGRYLYRLLVGERGMDARTILVTDLDADYRVHPQYFT
                     YLTWMHVTDPNRETQLYQPIPYLHNNIWEAPAMQRLFAAVLTQLQMWRSVLPEKLQSF
                     GSYSTTLHVVHDVGYWATDAIPEDSRFYWKCYFRYGDRFRAVPLFIPIYGDAVRARTY
                     WRSMATQYLQVRRWAWGVTDIPYVIDNAVRHTEIPLWSRVWRLLNLFGEHINWAISPF
                     VLMFGATLPILINPAFAQTTLGQNLPVYASAMLTLGLGVLLVLVFVENQIVPPKPRSW
                     SPLRRLLTWLQWFALPIVGVIFSCLPALDAQTRLLTGRYLEYKVTEKA"
     gene            1155..>1704
                     /locus_tag="VHH53_06670"
     CDS             1155..>1704
                     /locus_tag="VHH53_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289866.1"
                     /translation="MTDETVVAIANIPLARSLRRVLAVPAIALLAGGLAAVVGWLVGG
                     LPGLALAVAGGVLIVVASYLALLVLSVRLDLEVSTLRLRRLGSEQRFSLVRGPVTRVP
                     LRGVGAARLRPRFGALGWGLGPARLRDEEEIQLVRLAPTTTAILVPTDSGRVAIAPAS
                     EQQLIAALGAAARVQQRLDQVAA"
BASE COUNT          235 a          603 c          573 g          293 t
ORIGIN      
        1 ttcgcgcgct ggccgaggcg gcgtggccga cggagcgcaa ggtctgcgcg atcatcaccc
       61 gcgagaccga cgagggcggg atcgagaacg tccgcgtcct tcgcgaggag tacggcgacg
      121 ccttcgccga gttcatccac atcctcgacc cgctcgagcc gggcatcgtc gttggcaaga
      181 gcagcgcgat ggcgtggggc ggccgctacc tctaccggct gctggtcggg gagcggggga
      241 tggacgcgcg gacgatcctg gtcaccgatc tcgacgccga ctaccgcgtc cacccgcagt
      301 acttcacgta cctgacctgg atgcacgtca ctgatccgaa ccgcgagacc cagctctacc
      361 agccgatccc gtacctccat aacaacatct gggaggcacc ggccatgcag cgcctgttcg
      421 ccgcggtgct gacccagctg cagatgtggc gcagcgtcct gcccgagaag ctgcagagct
      481 tcggcagcta ctcgaccacc ctgcacgtgg ttcacgacgt aggctactgg gccacggatg
      541 cgatcccgga ggacagccgc ttctactgga agtgctattt ccgctacggg gaccgcttcc
      601 gggccgtgcc gctcttcatc ccgatctacg gcgatgccgt ccgagccagg acctactggc
      661 gctcgatggc gacccagtac ctgcaggtgc gccgctgggc atggggcgtg accgacatcc
      721 cgtatgtgat cgacaacgcg gtgcgccaca ccgagatccc gctctggtcg agggtgtggc
      781 gcctgctcaa cctgttcggc gagcacatca actgggccat ctcgcccttc gtcctgatgt
      841 tcggggccac gctgccgatc ctgatcaacc cggccttcgc ccagacgacc ctgggccaga
      901 acctgccggt gtacgcgtcg gccatgctga ccctcggcct gggcgtcctg ctggtgctcg
      961 tcttcgtcga gaaccagatc gtccccccca agccgcggag ttggagcccg ctgcggcggc
     1021 tgctgacctg gctgcagtgg ttcgcactgc cgatcgtggg cgtcattttc agctgcctgc
     1081 cagcgctcga cgcgcagacg cggctgctga ccgggcgcta cctcgagtac aaggtcaccg
     1141 agaaggcctg accaatgacc gacgagacgg tcgttgccat cgccaacatc ccgctggcgc
     1201 gatccctgcg ccgggtgctg gcggtgcccg ccatcgctct gctggccggt gggctggctg
     1261 ccgtcgtggg atggctggtg ggcgggctgc ccggcctcgc cctggccgtc gcgggcggcg
     1321 tgctgatcgt ggtggcgtcc tatctcgccc tgctcgtcct gagcgtaagg ctggacctgg
     1381 aagtctccac ccttcgcctc cggcggctgg gctccgagca gcgcttctcc ctagtgcgag
     1441 ggccggtgac gcgcgttcct ctgcggggag tgggtgcggc gcgcctccgc ccgcgcttcg
     1501 gcgcgctggg ctggggcctg ggtcccgccc gcctgcgcga cgaagaggag atccagctgg
     1561 ttcggctggc cccgaccacc acggccatcc tggtgccgac tgattcgggc cgcgtcgcca
     1621 tcgctccggc gtccgagcag cagctcatcg ccgcgctggg tgccgcggct cgcgtgcagc
     1681 agcgactgga tcaggtcgcg gcgc
//
LOCUS       DATADN010000512         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_203051, whole genome shotgun sequence.
ACCESSION   DATADN010000512 DATADN010000000
VERSION     DATADN010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203051"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2648)
                     /locus_tag="VHH53_06675"
     CDS             complement(<1..>2648)
                     /locus_tag="VHH53_06675"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase"
                     /protein_id="HEX2289867.1"
                     /translation="GQAQNQAYGYLFPHGTFFLLGDMLNVPGWVTQRLWWALLLVVGF
                     WGLLRVAEALGIGSPTSRVIAAVAFALSPRVLTTLGAISSETLPMMLAPWVLLPVILA
                     LRGHGEVRVMAARSAVAIALMGAVNAVATLTGCLAAVIWLACHRPNKLWWRFTGWWLL
                     CAVLAVLWWVVALVMLGRVSPPFLDFIESSGVTTQWMSLTEMLRGTDAWTPFVAPNAT
                     AGASLVTGSVAVLATTLVAAAGLAGLAMRTMPARGRLITILLSGVVLLAVGYSGGLGS
                     PVALEVQAFLDAAGTPLRNLHKLEPLLRLPLALGLAHLLGRIPLPGSAPRPVWTNALA
                     HPEQDKRVAVGVVVLVALTAATSLAWTGRLTPPGAFDAIPPYWQATADWLDEHNDGGR
                     VLVAPGAPFATQVWGTSHDEPLQVLGDNAWGVRDSIPLTPPQTIRALDSVQRLFAAGR
                     PSAGLADTLARQGISYVVVRNDLDPETSRSARPVLVHRAIDGSPGLDKVAEFGEPVGP
                     GTLKGFVTDSGLRPKYPAVEIYRVRPATPVAPYSVDADAMARVDGAPEALLRLDERRR
                     LLGQPSLGPMMLTADAQRAGLPVPVVTVTDTPLARETDYGRVDHHSSAIRAHGDARHT
                     FNRVIDYPAPGADTVHGQWNGRRVSVSSSAADSTALPNVAPATGPAAAIDADSSTSWV
                     SNALQSAIGQWLQVDFDRPVTNATVTLTPSATAVGAQIRRIEVSTANGTSTLRFDEAG
                     KPVTAALPYGETPWVRFTAVATEDGSPGVQFGITDFSVTQYDANGFAHPVNLRHTVSV
                     PGPPPGSAVAQWDLGSELLGRPGCAAGPTGTRCAALMALAPEEPVNLSRTLSVPESVA
                     VTPTVWVRPRQGPKLADLISEPGTARA"
BASE COUNT          433 a          954 c          917 g          344 t
ORIGIN      
        1 gcgcgtgcgg tgcccggttc gctgatcagg tcggccagct tggggccctg ccgggggcgc
       61 acccacaccg tcggcgtcac cgccaccgat tcgggcaccg acaacgtccg gctgaggttg
      121 accggttcct ccggcgccaa cgccatcagc gccgcgcacc gcgttcccgt cggtcctgcc
      181 gcgcagcccg gccggcccag cagttcggag cccagatccc attgcgccac agcagaaccc
      241 ggcggtggcc ctggcaccga aacggtgtgg cggagattca cgggatgcgc gaagccattc
      301 gcgtcgtatt gggtgaccga gaaatcggtg atgccgaact ggacgccggg ggatccgtct
      361 tcggtggcta ccgcggtgaa ccgcacccac ggcgtctccc cgtacggcag ggccgcggtg
      421 acgggcttgc ccgcctcgtc gaaccgaagc gtgctggtgc cgttggcggt cgacacctcg
      481 atgcgccgga tctgcgcgcc gacggcggtg gcgctcggcg taagtgtgac cgtcgcgttg
      541 gtgaccgggc ggtcgaaatc gacctgcaac cattggccga tcgcggactg caacgcgttg
      601 gacacccagc tggtggacga gtcggcatcg atcgccgcgg ccggaccggt cgcgggggcg
      661 acgttgggca gcgcggtgga gtcggccgcc gaactcgaca ccgagacgcg cctaccgttc
      721 cactggccgt gcaccgtgtc ggcgcccggc gccggatagt cgatcacgcg gttgaacgtg
      781 tgccgcgcat cgccgtgcgc gcggatcgcc gaagagtggt ggtcgacgcg gccgtagtcg
      841 gtctctcgcg ccaacggggt gtcggtcacg gtgacaaccg gcaccggcag gcccgcgcgt
      901 tgcgcgtcgg cggtcaacat catcgggccg agcgaaggtt gaccgagcaa cctgcgtcgt
      961 tcgtcgagcc gcagcagcgc ctcgggcgca ccgtccactc gtgccatcgc gtcggcatcg
     1021 accgagtacg gcgccaccgg tgtcgcaggc cgcacccggt agatctcgac agccggatac
     1081 ttgggccgca gtccactgtc ggtgacgaac cccttcaggg tgcccggccc gacgggttca
     1141 ccgaactccg cgaccttgtc cagcccgggt gaaccgtcga tcgcgcggtg caccagcacc
     1201 gggcgcgccg accgcgacgt ctccggatcc agatcgttgc gcaccacgac gtacgaaatg
     1261 ccctgtcggg caagcgtatc cgccagtccg gcggacggcc gccccgcggc gaacaggcgc
     1321 tgaaccgagt cgagcgcgcg gatggtctgc ggcggagtca acggaatcga atcccgcacc
     1381 ccccaggcgt tgtcgccgag cacctgcagt ggctcgtcgt ggctggtgcc ccacacctgg
     1441 gtggcgaacg gagcaccggg ggccaccagt acccgaccgc cgtcgttgtg ctcgtcgagc
     1501 cagtcggcgg tcgcctgcca gtacggcggt atcgcgtcga acgcgccggg tggggtgagc
     1561 ctgcccgtcc aggccagcga ggtggccgcg gtcagcgcga cgagcaccac caccccgacg
     1621 gcgacccgct tgtcctgttc ggggtgcgcc aatgcgttgg tccacaccgg ccgaggagcg
     1681 ctgcccggca acggaatacg gcccagcagg tgcgccaggc cgagcgcgag tggcagccgc
     1741 agcagcggct cgagcttgtg caggttgcgc aggggtgtgc ccgccgcgtc gaggaacgcc
     1801 tgcacctcga gagcgaccgg tgaccccaga ccgccggagt agccgacggc gagcaggacg
     1861 acgccgctca acaggatggt gatgagccgg ccgcgcgccg gcattgtccg catcgccagc
     1921 ccggcgagac ccgcggcggc caccagcgtc gtcgccagca ccgcaacgga tcccgtcacc
     1981 aacgatgcgc ccgcggtcgc gttgggcgcg acgaacggtg tccacgcgtc tgtgccccgc
     2041 agcatctcgg tcaacgacat ccactgcgtg gtgaccccgg aggactcgat gaagtcgagg
     2101 aacggtgggc tgacgcggcc cagcatcacg agcgcgacga cccaccacag cacggcgagc
     2161 accgcacaca acagccacca accggtgaac cgccaccaca acttgttggg ccggtgacac
     2221 gccagccaga tcaccgcggc cagacagccg gtcaacgtcg ccacggcgtt caccgcgccc
     2281 atcaacgcga tcgccacggc ggaccgggcc gccatcaccc gcacctcccc gtgtccgcgc
     2341 agcgcgagga tgaccggcag caacacccac ggcgccagca tcatcggcag cgtctccgac
     2401 gagatggcgc ccagcgtggt gagcacgcga ggggacagcg cgaatgccac cgcggcgatc
     2461 acgcgtgacg tggggctgcc gatgcccaac gcctcggcga cccgcagcag cccccagaac
     2521 ccgacgacca gcagcaacgc ccaccacagc cgttgggtga cccagccggg gacgttcagc
     2581 atgtcgccga gcaggaagaa ggtgccgtgc gggaagaggt atccgtacgc ctggttctgt
     2641 gcctggcc
//
LOCUS       DATADN010000513         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_372332, whole genome shotgun sequence.
ACCESSION   DATADN010000513 DATADN010000000
VERSION     DATADN010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372332"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1880
                     /locus_tag="VHH53_06680"
     CDS             261..1880
                     /locus_tag="VHH53_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2289868.1"
                     /translation="MTLRTSRRRVAQTALATAAVATLPRSRAAAAQATPVPGEAAITF
                     AQVQSAVDRLDGLIEDGMAQTGLPGAAVAVVYNDEVVYERGFGVREVGKPEPITPETV
                     FQIASLSKSISSTLVAAVVGDDTTTWDATIGSLEPGFALADPWVSDQVTIRDMLCHRS
                     GLPAFGGDPLVFNFGYDRDECLRRLRYYSLATPFRTTWAYSNLGFSAGAYAAAQAAGE
                     SWEDLAETRLFAPLGMTSSSYRFADFGRHENRAAPHYRTRDGVWELGDLTDDDAATPA
                     GGVSSNLRDLTHWLRLHLAGGTFDGQEVVANDPLRETYRPQIVAFSPPDPTAGPTAFY
                     GLGWFVSYDDRGRLEVTHGGDFNSYSTQATLLPGSGLGILVLCNGGASAVRGAIPKAF
                     LEMVTKGEPSQDWVSAIETSFAAGLASILATSTQFPQGEPPADAAPPLPLDAYTGTFT
                     NTTYGEVTVRDEAGGLVLKFGPNGVQHQLNAWDRDAFSYLLTGSEGAQLAQYGVLFTV
                     CPEGQADSALVSLAGVGPDAAATFTRVTANA"
BASE COUNT          336 a          684 c          680 g          352 t
ORIGIN      
        1 aagatttcgc cgcaagatcg atcacctccg tgccgcggta ggtccagaaa aggcaacagg
       61 ggcaacccct cctggtgagg ctggctcaga gcggtctctt tggtgcgacg atcgcttcgg
      121 cgcattgacc gtcagaccca catacgcgtt cctgtggacg ggaacggcat gcgtcgtgtc
      181 ctcgcactcc agtcgccttc aaacctatgg ggtgagcaat ggctcgtgaa cgtcctggaa
      241 acacatggaa ggatgcaccg atgacgctcc gcacctcacg ccgtcgcgtc gcgcagacgg
      301 cactcgccac cgccgccgtg gccacgttgc cccgttctcg ggcggccgct gcacaggcaa
      361 cgccggttcc cggcgaggcg gcaatcacct tcgcccaggt ccagtctgcc gtggatcgcc
      421 tcgatggcct gatcgaggat ggcatggccc agaccggtct ccctggggca gccgtggcag
      481 tggtctacaa cgacgaggtg gtctacgaac ggggcttcgg ggtgcgcgag gtcggcaagc
      541 ccgagcccat cacgccggag accgtcttcc agattgcctc gctttcgaag tccatctcct
      601 ccacgctggt cgccgctgtc gtcggcgacg acacgaccac ctgggacgcc accattggca
      661 gcctcgagcc cggcttcgcg cttgccgacc cgtgggtgag cgatcaggtc accatccgcg
      721 acatgctctg ccatcgctcc ggcttgccag ccttcggcgg agacccgctg gtcttcaact
      781 ttggctacga tcgcgacgag tgcctgcgac gactgcggta ctactccctg gccactccct
      841 tccgcaccac ctgggcctac tccaatctgg ggttcagcgc cggtgcctat gcagcggctc
      901 aagcggcagg ggagtcctgg gaggatctgg ccgagacgcg gctgttcgcg ccactgggta
      961 tgaccagctc cagttaccgc tttgcggatt tcggccgtca cgagaaccgg gccgcgccgc
     1021 actaccgcac gcgggatggc gtctgggaac ttggcgacct gaccgatgac gatgcggcga
     1081 cgcccgcggg aggcgtgagc tccaacctgc gggatctcac ccactggctg cggctgcacc
     1141 tggccggggg aacgttcgac gggcaggagg tggtggcgaa cgacccgttg cgcgagacct
     1201 accgaccgca gatcgtggcg ttctcacccc cggatccgac ggccggtccc accgcgttct
     1261 acggactggg atggtttgtc tcctacgacg accggggccg gctggaagtc acgcacggcg
     1321 gagatttcaa cagctacagc acgcaggcta cgctgctgcc gggatcgggc ctgggcatcc
     1381 tggtgctgtg caacggtggg gccagcgcag tgcgcggagc catcccgaag gcgtttctgg
     1441 agatggtcac gaagggcgaa ccgagtcagg actgggtgag cgcgatcgag acctcatttg
     1501 ccgcggggct ggccagcatc ctggcgacct cgacccaatt cccccagggc gagccacccg
     1561 ccgatgcggc tccgcccttg ccactcgatg cctacaccgg cacctttacc aacacaacgt
     1621 acggtgaggt gaccgtgcgg gatgaggccg gcggtctggt gctgaagttc gggccgaatg
     1681 gggttcagca ccagctgaac gcctgggacc gcgacgcgtt cagctatctg ctcaccggga
     1741 gcgaaggggc ccagctcgcc cagtacggcg tgctcttcac cgtctgtccg gaaggtcagg
     1801 cggactcggc gctggtgagc ctggcgggag ttggtcccga cgcggcggcg accttcacac
     1861 gggtaacagc caacgcgtga ggccggccgt ctccgggagt tgctcctgat gagggtcgac
     1921 aaacggaggc ttagccggca tgcaacagtc actgactggt cggttctcgt agtggcgggg
     1981 cgctgcggtg cctcccatgc tagagtgcgg gccgttccta acccaaccgc gtccgggggt
     2041 ccgccatgct cg
//
LOCUS       DATADN010000514         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_372607, whole genome shotgun sequence.
ACCESSION   DATADN010000514 DATADN010000000
VERSION     DATADN010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372607"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..383)
                     /locus_tag="VHH53_06685"
     CDS             complement(6..383)
                     /locus_tag="VHH53_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2289869.1"
                     /translation="MMNFLVKTIANAGALAVAVWLLDKITLTGDSTGKKTGTLILVAL
                     IFGLVNFLVKPVVKLLSLPLLILTLGLFTLVVNALMLLLTSWLADQFDLSFHVEGFWT
                     AVLGGLIISVVSWALNVVLPDGD"
     gene            444..761
                     /locus_tag="VHH53_06690"
     CDS             444..761
                     /locus_tag="VHH53_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2289870.1"
                     /translation="MKAFRLDELEAERAANEGAYLQFLREQNMSVGLYALDAGDQDPQ
                     KPHNQDEVYFVVSGRAAITVGLETTQVARGSVIYVPAGVAHKFHHITEDLRVLVVFSP
                     PES"
     gene            926..1138
                     /locus_tag="VHH53_06695"
     CDS             926..1138
                     /locus_tag="VHH53_06695"
                     /inference="COORDINATES: protein motif:HMM:NF036459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5326 family protein"
                     /protein_id="HEX2289871.1"
                     /translation="MREIFTGLPWWVKWIAVPVIALVVFGGLIASVVGFVIGLLFKVL
                     VFVALVGGLIYVVRKFTSSSSSRSDW"
     gene            1346..>2097
                     /locus_tag="VHH53_06700"
     CDS             1346..>2097
                     /locus_tag="VHH53_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX2289872.1"
                     /translation="MAAADTARVHLHTVPAQPRSAPPSEQSPPATPPPPATLIGSVQR
                     AMRLLETVAAHEYGAPAKQLARETGLALPTTYHLLRTLVHEGYLCRDKGLFFLGEAAE
                     RLSSSGARQKRRSTVVDALAHWRDTLGVPVYYAVYREGEIELMCVSDTPANPAVEEWA
                     DFRETGHAHAIGQCLLSQLDEEARRDHLDRYPVQPLTPYTVRDNHSLLRRLERVRRME
                     PVTERQEYALGTVCAAIPITLGTTAATMAISMP"
BASE COUNT          364 a          690 c          724 g          319 t
ORIGIN      
        1 ggcgttcagt ccccgtcggg caggacgacg ttcagcgccc aggacacgac cgagatgatc
       61 aggccaccca ggacggccgt ccagaagccc tccacgtgga agctcaggtc gaactggtcg
      121 gccagccacg aggtgagcag cagcatcagc gcgttgacca ccagggtgaa caagccgagc
      181 gtcaggatga gcagcggcag gctgaggagc ttgacgaccg gcttgaccag gaagttcacc
      241 agaccgaaga tcagggcgac gagaatcagg gtgccggtct tcttgcccgt gctgtcaccg
      301 gtgagggtga tcttgtcgag cagccatacg gcgaccgcca gggcacccgc gttggcgatc
      361 gtcttgacta ggaaattcat catgtgtctg atcgtggcag acctgatcgg acatgggcac
      421 aagaggcagg ggcggacaag gcgatgaagg cattccggct ggacgaactg gaggcggagc
      481 gcgccgccaa cgagggcgcc tacctgcagt ttctgcggga gcagaacatg tcggtcggcc
      541 tgtacgccct cgacgcgggc gaccaggatc cacagaagcc gcacaatcag gacgaggtct
      601 acttcgttgt gagcggccgt gccgcgatca ccgtcggcct ggagacgacg caggtggcac
      661 gcggcagcgt gatttacgtg ccggcgggcg tcgcccacaa gttccaccac atcaccgagg
      721 acctgagggt cctggtcgtg ttctctccgc ctgagagctg aggcgccgcc tcggggttcc
      781 ctaggggttc ggtgagggga ggacaagggg tgcgaggccc ccgcgggatc ccttcccgac
      841 ctagcatcga gtgcaggaca tcagagatcc agaagtaccg acaggacccg gcactcgaac
      901 gggtggagag gtaaggacga gggcgatgcg agagatcttc acgggactgc cgtggtgggt
      961 gaagtggatc gcggtgccgg tcatcgccct ggtcgtgttc ggcgggctga tcgccagcgt
     1021 ggtcgggttc gtgatcggac tgctcttcaa ggtgctggtc ttcgtggcgc tggtcggtgg
     1081 actgatctac gtcgtacgga agttcacgtc gagctcctcg tcgcgcagcg actggtgaga
     1141 ccggtgggaa accggtgggg gtagcgggaa acacccggcg tgaccggttc cctcggccgg
     1201 gggaagtttc cgttccgggc cgtctgcgtg cggtggcaga cgaatagagt ccggaactcg
     1261 acgcggggac atcccccgcg agcggcgtac atccccaccc gtgtccatcg gcacggtcta
     1321 ccccctcgcg cttcgggtgt gacccttggc cgcggctgac accgcacgtg tccacctgca
     1381 caccgtcccc gcgcaacccc gctcggcgcc cccctccgag caatcgcctc ccgccacgcc
     1441 gccccctccg gcgaccctca tcggctccgt gcagcgcgcg atgcgcctgc tggagaccgt
     1501 cgcggcgcac gaatacggcg cccccgccaa gcaactggcc cgggagaccg ggctggccct
     1561 gcccaccacc taccacctgc tgcgcaccct cgtgcacgag ggctatctgt gccgggacaa
     1621 gggcctgttc ttcctgggcg aggccgccga gcggctgagc agcagcgggg cacggcagaa
     1681 acgtcgcagc acggtcgtcg acgccctcgc gcactggagg gacacgctcg gtgtgcccgt
     1741 gtactacgcg gtgtaccgcg agggcgagat cgagctcatg tgcgtctccg acaccccggc
     1801 caatcccgcg gtcgaggagt gggccgactt ccgggagacc gggcacgcgc acgccatcgg
     1861 gcagtgcctg ctgtcccagc tggacgagga ggcccgccgc gaccacctcg accgctaccc
     1921 ggtgcagccg ctcacgccgt acacggtgcg ggacaaccac agcctgctcc ggcgtctgga
     1981 acgggtgcgg cggatggaac cggtgaccga acgtcaggag tatgccctgg gcacggtctg
     2041 tgcggcgatt cccatcaccc tcggcaccac ggccgcgacg atggccatct cgatgcc
//
LOCUS       DATADN010000515         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_805628, whole genome shotgun sequence.
ACCESSION   DATADN010000515 DATADN010000000
VERSION     DATADN010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805628"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VHH53_06705"
     CDS             complement(<1..935)
                     /locus_tag="VHH53_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX2289873.1"
                     /translation="MTEVLPAGDVAQYAWLLVALPAFGALVLLVGGRRTNAWGHLLGC
                     ATVLASFVYGVVLFFEWLSLGAEERTRELGVGTWFGVNSLQVDFGLRLDPLSLTFMLL
                     ITGVGGLIHIYSIGYMSHDPGRRRFFGYFNLFIVAMLILVLADNFVTLYLGWEGVGLA
                     SYLLIGWYQNRPSAATAAKKAFLMNRVGDVGLALAVFLIWTSLGTVQFSEVFARVGEI
                     SGPTLLAITLLLLLGACGKSGQFPLQAWLPDAMEGPTPVSALIHAATMVTAGVYLIAR
                     SHEIYNASPDGRLVVMLVGAVTLLIGAIIGCAYDD"
     gene            complement(1024..1323)
                     /gene="nuoK"
                     /locus_tag="VHH53_06710"
     CDS             complement(1024..1323)
                     /gene="nuoK"
                     /locus_tag="VHH53_06710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX2289874.1"
                     /translation="MTPTYYLLLSALLFTIGAAGVLVRRNAVVVFMCIELMLNAVNLS
                     LITFSRINGTLDGQIMAFFVMVVAAAEVVVGLAIIMAIFKTRRSASVDDANLLKY"
     gene            complement(1320..2150)
                     /locus_tag="VHH53_06715"
     CDS             complement(1320..2150)
                     /locus_tag="VHH53_06715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX2289875.1"
                     /translation="MIGLGETVAFWILGPLALAGGLAMVFARNAVHSALWLAQTMLCL
                     GALYMVQQAPFLGFVQIIVYTGAIMMLFLFVLMLVGKESVDSVVEVLRGQRVAAALAG
                     IGLAALLVAMLGRALTGVTAAGLIGVNTTSGGNVANLGRILFTDYLFPFELTSALLIT
                     AAVGAMVLTFTERTRESSPTQRERVEARIRGTRPSPLPGPGVFATANSVATPALLPDG
                     SVAPESMSELLESTSRERLDDDLRSVTDAGTQPDSRALRGPVEHIEPTGPEEPQEGSR
                     "
     gene            complement(2210..2749)
                     /gene="nuoI"
                     /locus_tag="VHH53_06720"
     CDS             complement(2210..2749)
                     /gene="nuoI"
                     /locus_tag="VHH53_06720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX2289876.1"
                     /translation="MGLLDPVKGFGVTFSTMFKKVVTEEYPEVKKITAPRFHGKHQLN
                     RHPDGLEKCVGCELCAWACPADAIYVEGADNTDKARYSPGERYGEIYQINYLRCIGCG
                     LCIEACPTRALTMTNEYELADDNRRDLIYTKDDLLAPLLAGMEQPPHPMRLGDSEEDY
                     YVQGRTLERRSGSVGEEER"
BASE COUNT          540 a          932 c          904 g          428 t
ORIGIN      
        1 atgtcgtcgt aggcgcagcc gatgatcgcc ccgatcagca gggtgacggc gccgaccagc
       61 atcacgacca gccggccgtc cgggctggcg ttgtagatct catgggagcg ggcgatcagg
      121 tacaccccgg cggtcaccat cgtcgcggcg tggatcagcg ccgagaccgg ggtcgggccc
      181 tccatcgcgt cgggcaacca ggcctgcagc gggaactgac cggacttgcc gcaggccccg
      241 agcagcagca gcagggtgat cgccagcagg gtggggccgc tgatctcccc aacccgagcg
      301 aacacctcgg agaactgcac ggtgcccagc gacgtccaga tcaggaagac cgccagcgcg
      361 aggccgacgt caccgacccg gttcatcagg aaggccttct tcgccgccgt cgcagcgctc
      421 gggcggttct gataccagcc gatgagcagg taggaagcca gacccacgcc ttcccagccg
      481 aggtacagcg tgacgaagtt gtccgcaagc accaggatga gcatcgccac gatgaacaag
      541 ttgaagtacc cgaagaaccg gcgccgacca ggatcgtggc tcatgtagcc gatcgagtag
      601 atgtggatca atccgccgac gccggtgatg agcagcatga aggtcagtga cagcgggtcc
      661 agccgcaggc cgaagtcgac ctgtagggaa ttgaccccaa accaggtgcc cacccccaat
      721 tcccgggtcc gttcctcggc gcccaacgag agccactcga agaacagcac gacgccgtaa
      781 acgaaagagg cgagcaccgt cgcgcagccc agcaggtgcc cccaggcatt ggtgcggcgg
      841 ccaccaacca gcagcaccag cgcaccgaag gccggcagcg cgacgagcag ccacgcgtac
      901 tgcgccacgt cgcccgcggg caacacctcg gtcaccgcgc ccccttcagc aagtctttcg
      961 tgtcaacgat tgggccgcac gcaggtctct cgtcacaatg cttcggcccc ggtcaagcag
     1021 gcctcagtac ttgagcaggt tcgcgtcgtc gactgaggcc gatcgacggg tcttgaagat
     1081 cgccatgatg atggccagac cgacaaccac ctcggcggca gccacgacca tcacgaagaa
     1141 cgccatgatc tgcccatcca gggtgccatt gatccgggag aaagtgatca agctcaggtt
     1201 caccgcattg agcatcagct cgatgcacat gaacaccacg acggcgttgc ggcgcaccag
     1261 cacgcctgct gcgccaatgg tgaacagcag cgccgacagc agcaggtaat acgtcggcgt
     1321 caccggctcc cctcctgggg ctcctcgggt ccggtgggtt cgatgtgttc gaccggaccg
     1381 cgtaaggcgc gagaatcagg ctgcgttcca gcatcggtaa cgctgcgcag gtcgtcgtcc
     1441 agacgctcgc gggaggtgga ctcgaggagt tcggacatcg actcgggggc caccgaaccg
     1501 tccggcagca aggcgggggt agccaccgag ttggcggtgg cgaagacgcc gggaccaggc
     1561 agcggggagg gacgggtacc gcggatccgg gcctccaccc gctcccgttg ggtcggactg
     1621 ctctctctgg tgcgctcggt gaacgtcaac accatcgcgc cgaccgcggc ggtgatcagc
     1681 aacgccgagg tgagctcgaa ggggaaaagg tagtcggtga acaggatgcg accgaggttg
     1741 gcgacgttgc cgccgctggt ggtgttgacc ccgatcaacc ccgctgcggt gaccccggtc
     1801 aaggcccggc ccagcatcgc caccaggagc gcagccagcc cgatcccggc cagcgccgcg
     1861 gcgacccgct gaccgcgcag cacctcgacc accgagtcga cgctttcctt gcccaccagc
     1921 atcagcacga acaggaacag catcatgatc gcgccggtgt agacgatgat ctgcacaaag
     1981 cccaggaacg gcgcttgttg gaccatgtac agcgcaccaa ggcacagcat ggtctgtgcc
     2041 agccacagcg ccgaatgcac cgcgttgcgg gcgaatacca tggccagccc gccggccagc
     2101 gcgagcggac ccaggatcca gaacgcgact gtctcgccaa ggccgatcat gtcggtcgac
     2161 accgggttag gtagcggtac ggggggcgtg agcaggacag cgagcgtggt caccgctcct
     2221 cctcgcctac ggaacccgaa cgccgctcca aggtccgacc ttgcacgtag tagtcctctt
     2281 cactgtcgcc cagccgcatc gggtgggggg gctgctccat tccggccagc agcggtgcga
     2341 gcaggtcgtc cttggtgtag atgaggtctc gacggttatc gtctgccaac tcgtactcgt
     2401 tggtcatggt cagtgcccgg gtcgggcagg cttcgatgca caagccgcag ccgatgcagc
     2461 gcaggtagtt gatctgatat atctcgccgt agcgctcccc gggtgagtag cgcgccttgt
     2521 cggtgttgtc cgcgccttcg acgtagatcg cgtccgccgg gcaggcccag gcacacagct
     2581 cgcaccccac gcacttctcc agcccatccg ggtgtcgatt gagctggtgc ttaccgtgga
     2641 agcgcggtgc ggtgatcttc ttgacctcgg gatattcctc ggtcacgacc ttcttgaaca
     2701 tcgtcgagaa ggtgaccccg aagcccttga ccggatcaag tagtcccatg gccgtcctcc
     2761 tgcgtcatcg gtggcccgct ggtggtgacg gcagcgtgcc ggcg
//
LOCUS       DATADN010000516         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_805740, whole genome shotgun sequence.
ACCESSION   DATADN010000516 DATADN010000000
VERSION     DATADN010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805740"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..526
                     /locus_tag="VHH53_06725"
     CDS             191..526
                     /locus_tag="VHH53_06725"
                     /inference="COORDINATES: protein motif:HMM:NF021496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2218 domain-containing protein"
                     /protein_id="HEX2289877.1"
                     /translation="MTTLSSRADVRTDAPARYAKQLVSHLGRKVEFVTDGPTWTATVN
                     DMTLAITVADAVLTLMVTGTDSTAVARGEHALGSHLERFGARRELSVTWTRTTTPAAA
                     ATDGEGALR"
     gene            523..1413
                     /locus_tag="VHH53_06730"
     CDS             523..1413
                     /locus_tag="VHH53_06730"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX2289878.1"
                     /translation="MTSTTARADALIPEAHPFKQHLHRVTAVDAAQPPRAWTPANGPM
                     PSIYLSHGAPPLFEDTPWMTQLKDWARALPRPKAILIVSAHWESAPLALSASRPADLV
                     YDFGGFSQIYFRMRYDTPDGSDLARQVAALLPQKVHQHTSRGLDHGAWVPLKVMYPAA
                     DIPVLQLSIPTHDAGALLGIGARLKALREQGVLVIGSGYMTHGLPFITPDMWQHNTVP
                     GWSSDFDAWAADALARGDVDELAAFRDHAPGMPYAHPTVEHFTPLFITLGAAADATAP
                     VTTTIDGFAIGLAKRSFQTA"
BASE COUNT          245 a          608 c          443 g          215 t
ORIGIN      
        1 cctcgtcgtc ctgcactaca acctcaagtt cttccccgat gacctcgggc aggccgtcgt
       61 cgacatcttc cgcgtccagg acgggcggat cgtcgagcac tgggacgtcc tgcagccagt
      121 tccggccgag tcggccaaca ccaacgggat gttctgaccc acggccgcac gaccacgacc
      181 aggagacacc atgaccaccc tgtcctctcg cgccgatgtg cgcaccgacg ccccggcccg
      241 ctacgccaag cagctggtct cccacctggg ccgcaaggtc gagttcgtca ccgacgggcc
      301 gacctggacc gccaccgtca atgacatgac cctggccatt accgttgccg atgcggtcct
      361 gaccttgatg gtcaccggga ccgactcgac ggccgtggcc cgcggcgaac acgccctggg
      421 cagccacctc gaacgcttcg gtgcccgccg cgagctgagc gtcacctgga cccgcaccac
      481 cacccctgcc gctgccgcga ccgacggcga aggagcactg cgatgaccag caccaccgcc
      541 cgcgcggacg ccctgattcc tgaggctcat ccgttcaagc agcacctgca ccgcgtcacc
      601 gccgtcgacg ccgcccagcc gccccgggcg tggacgccgg cgaacgggcc gatgccgtcg
      661 atctacctga gccacggcgc cccgccgctg ttcgaggaca ccccctggat gacccagctg
      721 aaagactggg cacgcgccct gcccagaccg aaggcgatcc tgatcgtcag cgcccactgg
      781 gagtccgcgc ctctcgcgtt gtccgccagc cggcccgccg acctggttta cgacttcggc
      841 ggcttcagcc agatctactt ccggatgcgc tacgacaccc ccgacggcag tgatctggcc
      901 cgtcaggtcg ccgcgctgct gccgcagaaa gtgcaccagc acaccagccg cggtctggac
      961 cacggtgcct gggtgccgct gaaggtgatg tacccggccg cggacatccc ggtgctgcag
     1021 ctgagcattc ctacccacga cgccggcgcg ctcctgggca tcggggcgcg gctcaaggct
     1081 ctgcgggagc agggtgtgct ggtcatcggc tccggctaca tgacccacgg cctgccgttc
     1141 atcacccccg acatgtggca gcacaacacc gttcccggct ggtcgtcgga cttcgacgcc
     1201 tgggccgcgg acgccctcgc ccgcggcgat gtcgacgagc tcgcagcctt ccgcgaccac
     1261 gccccgggca tgccgtacgc ccacccgacc gtggagcatt tcacgccgct gttcatcacc
     1321 ctcggcgctg cggcggacgc caccgcgccg gtcaccacca ccatcgacgg cttcgccatc
     1381 ggcctcgcca agcgctcctt ccagacggca tgaccatcga caccgccgct gtcaccacag
     1441 ccgtgttgcc gaggaaagga tgaaccggtg accgacgccc gacccgccca cgccaccgac
     1501 acgcccacct c
//
LOCUS       DATADN010000517         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1256554, whole genome shotgun sequence.
ACCESSION   DATADN010000517 DATADN010000000
VERSION     DATADN010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256554"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..>1858)
                     /locus_tag="VHH53_06735"
     CDS             complement(174..>1858)
                     /locus_tag="VHH53_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2289879.1"
                     /translation="RLLEGLPIAIEFAADRLDVLSIEQLAERLTEGIYPLLHTSRRAT
                     REAQRTLRALADSSYRLCTDDERALLARLAVFSGRFQLEAVAPVCAGGPVSEDSVLTL
                     ITGLVDKSILVRDEHPGDSWYRLPALLRDYAHQYLMHSGEWASLQRRHAQWCVSLAEQ
                     AGVGLVSSRTDRWVGQVRGNHDNIRSAFEFCLRNGLPGEAVRLAVGLADYWMIESRTA
                     EGRRWLAQALAEPSLSPGDRSAALTIAAYLAIIDDDDAADGLISAARETGCHRAPSGP
                     GNLLFVTALRASQRGELDDIHGDLTEALTQFRAKGDIMGISRTLWLLGAVSIWNGDPA
                     ASLRYTDEFIHTPGVAGDNWGAGFIHFTMALAKVQLGDVLGGLADQRRCAEAVRRMDD
                     RLGMLWCVRSSPIVLLANDRPAEAAMMMGAGDQHHVFTLRAMDQLRDRCARSLRDKLG
                     QSQFDDLAAQGARLSLREAIDIIAERHSDQVSEDLQDETTLSSREWEVASLIAEGRSN
                     KEIAAALVISTRTAEGHVRRILNKLDLLSRSQVAAWMAEHRALHGRPRATTY"
BASE COUNT          343 a          597 c          606 g          312 t
ORIGIN      
        1 ccgaggtgac cctacggctt catgccgcga cggagcacgg cgttgacgac gccgagatcg
       61 gcgcggtggc tgagcttctg cgtgcagccg ttgccggcgc tggatgagag accacagtcc
      121 gacacaactg gggtgacctc ggccggtccc ccacgcaacg cgaaatccgg gtgtcaatag
      181 gtggtggcgc gaggacggcc gtgcagcgcg cgatgctcgg ccatccacgc cgcaacttgc
      241 gaccgtgaca gtaggtcgag cttgttgagg attcgccgga cgtggccttc ggcggtgcga
      301 gtggagatca ccagtgcggc agcgatctcc ttgttactgc ggccctcggc gatcagggag
      361 gcaacctccc actcacggga cgatagcgtt gtttcatcct gaagatcttc gctgacctgg
      421 tcgctgtgac gctcggcaat gatgtcgatg gcctcacgca acgacagacg tgcaccctgc
      481 gcggccaagt catcgaactg ggactgcccc agtttgtccc ggaggcttct tgcacatcga
      541 tcgcgtagtt ggtccatcgc acgaagtgtg aacacgtggt gctggtcgcc cgctcccatc
      601 atcatcgccg cttccgcagg ccggtcattg gccagcaaca cgatgggact gctgcggaca
      661 caccacagca tccccagtcg atcgtccatc cggcgcaccg cctcggcgca gcgacgctgg
      721 tcggcgagtc caccgagcac gtcgccgagc tgaaccttgg ccagcgccat cgtgaaatgg
      781 atgaaaccag caccccagtt gtcgccggcg accccggggg tgtggatgaa ctcatcggtg
      841 taccgcaggc tggcagcggg atcgccgttc catatggaca cggcgcccag caaccacagg
      901 gttcgcgaga tgcccatgat gtcgcccttg gcacggaact gagtgagggc ctcggtgaga
      961 tcgccgtgga tgtcgtccaa ctccccacgt tggctcgcgc gcagagcggt gacgaaaaga
     1021 aggttgccgg ggccggaagg agcgcggtga caaccggttt cgcgcgccgc ggagatcaac
     1081 ccatcggcgg cgtcgtcatc gtcgatgatc gcgaggtagg ccgcgatggt cagcgccgcc
     1141 gagcgatcac ccggcgacag gctgggctct gccagcgctt gagccagcca gcggcgtcct
     1201 tcggctgtgc ggctttcgat catccagtag tcggccagcc cgaccgccag gcgtacagcc
     1261 tcaccaggaa ggccgtttct cagacagaac tcgaacgccg acctgatgtt gtcgtggttg
     1321 ccccgaacct gaccaaccca acggtcggtg cgtgaggaca ccaagccgac accggcctgt
     1381 tcggctaacg agacacacca ctgtgcatgg cgacgctgga ggctcgccca ttccccggaa
     1441 tgcatcagat actgatgcgc gtagtcccgt agcagggcgg gcagtcgata ccaggaatca
     1501 ccggggtgct catctcggac caggatggat ttgtcgacca ggccggtgat cagggtcagc
     1561 accgagtcct cactgaccgg cccgcccgca cataccggcg caaccgcttc gagttgaaac
     1621 ctgccgctga acaccgcgag tcgggccaac aatgctcgct cgtcgtcagt gcaaagtcgg
     1681 tagctggagt ccgccagcgc ccgcaaggtg cgctgtgcct cgcgggtggc gcgccggctg
     1741 gtgtgcagca gcgggtagat gccctcggtg aggcgttcgg ccaactgctc gatggacaat
     1801 acgtcgagac ggtcggcggc gaattcgatc gcaatgggga gaccctcgag caagcgac
//
LOCUS       DATADN010000518         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_975723, whole genome shotgun sequence.
ACCESSION   DATADN010000518 DATADN010000000
VERSION     DATADN010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975723"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..879
                     /locus_tag="VHH53_06740"
     CDS             34..879
                     /locus_tag="VHH53_06740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX2289880.1"
                     /translation="MPETPRVSVIMIFLNAEPFLADAMGSVLAQTYDSWELLLVDDGS
                     SDASTARAQWAAAQAPGRVRYLAHAHHRNHGMSASRNLGLRHAQGRYIACLDADDVWV
                     PHTLAEQVARLEASPEAAMVYGPARWWYSWTGRPADGARDGLQPLAFPPDRVIEPPQL
                     LIRLLQQHQLYTPTGMLTWRTVLDRVGGFEPAFRGLYEDQVLLAKICLEAPVLQAQAC
                     WYHYRQHPASCCQVARSTGQNAAARLTFLHWLTTYVAARTGPQGDLWATLQQELWRAR
                     GATAM"
     gene            complement(1168..>1599)
                     /locus_tag="VHH53_06745"
     CDS             complement(1168..>1599)
                     /locus_tag="VHH53_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX2289881.1"
                     /translation="ARTDTTPVTSFATAGIRAFYGTIHIARMDQERSAMATPKQGSPR
                     KPAAAKQAAGRRAPSGAGQARGLRQPVRPSAELAAIVGQAPLPRTEITKKLWAYIKAH
                     GLQDAQNKRQINADPTLEPIFGGKPSISMFELAKLVSQHVQ"
BASE COUNT          234 a          515 c          551 g          299 t
ORIGIN      
        1 ccgtgatggc gcggacgatg gaggcggcag gagatgcctg agacgccccg ggtctcggtg
       61 attatgattt tcctgaacgc cgaaccgttt ctggcggacg cgatggggag tgtgttggcg
      121 caaacctatg actcctggga actgctgctg gtggatgacg gctcctccga tgcgagcacg
      181 gcccgtgccc agtgggcggc ggcccaggcc cctgggcggg tgcgctacct ggcgcacgcg
      241 caccaccgga atcacggcat gagcgcgtcg cggaacctcg gcctccgcca tgcgcagggc
      301 cgctatatcg cctgtttgga tgcggatgat gtctgggtcc cgcacaccct cgccgagcaa
      361 gtcgcacggt tggaggcctc ccccgaggcc gcgatggtct acggaccggc gcgctggtgg
      421 tacagctgga ccggccggcc ggccgatggg gcgcgggatg gtctgcaacc gctggccttc
      481 ccgccggacc gggtgatcga gccaccccag ctgctcatcc gcctgttgca gcagcaccaa
      541 ctatataccc cgacgggcat gctcacctgg cggacggtgc tcgaccgggt gggcggcttc
      601 gaacccgcct tccggggctt gtacgaggac caagtgttgc tcgccaagat ctgcctggag
      661 gcccccgtcc tgcaggccca ggcctgttgg tatcactacc gccagcaccc ggcgtcctgc
      721 tgccaagtgg cccgcagcac cgggcaaaat gcggcggccc gcctgacatt cctccactgg
      781 ctcacgacct atgtcgcagc ccggacgggt ccgcaggggg acctgtgggc gacgctgcag
      841 caggagctct ggcgcgcgcg gggtgcgacg gccatgtaac ggagcatggc atgagacttt
      901 tgagctgttc ggtctgattc tgtccatccc cagcgacgga agtatgcgac aaaccagccc
      961 gtgcgggctg gttattttgt atcgtactgt cgaattgtcg gtgaacgggt ctctagaacc
     1021 ttcaggcgcg tagcgatggg acgacccgag aggcccgacc gctctcctct ggggtcaggt
     1081 ctttggggag gcgatcccag agattcgtgc gaggcggagc ggcggcgtcg tgatccgagg
     1141 caccgtcccc cacccctgcg ggcggactta ctgcacgtgt tggctcacca gcttggccag
     1201 ctcgaacatc gagatggacg gtttcccgcc aaagatgggc tccagcgtgg gatccgcgtt
     1261 gatctggcgt ttgttctggg cgtcctgcag gccgtgggct ttgatgtacg cccagagttt
     1321 cttggtgatc tccgtgcgcg gcagcggcgc ctggcccaca atggctgcca gctcggcgct
     1381 cggccgtacg ggctgccgca gcccccgcgc ctgtccggca cctgacggcg ctctccggcc
     1441 cgcagcctgc tttgcggcgg ccggtttcct gggagagccc tgtttcgggg ttgccatagc
     1501 gctcctttcc tggtccatgc gggcgatgtg aattgtaccg tagaacgctc ggatgccagc
     1561 cgtggcgaat gacgtgaccg gggtcgtgtc tgtgcgagc
//
LOCUS       DATADN010000519         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1125976, whole genome shotgun sequence.
ACCESSION   DATADN010000519 DATADN010000000
VERSION     DATADN010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125976"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VHH53_06750"
     CDS             complement(<1..1443)
                     /locus_tag="VHH53_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2289882.1"
                     /translation="MPLAIRLIAGRLRHHRDELLTDVAADLADHTAALDALVAEHLSV
                     RTAFAWSYQLLTDEQRRAFRLLGWHPGPAITPVVITAVADVPPAQGRRLLRELMDHNL
                     LEQLSLAGVPGGPRYRMHDLVRLYARERANAEEPPTQHAAAVGRLADSYLAITRQADR
                     LLRPHVTDGSAQGTTSRAVLTFTDASQARSWLTIERLNLLGCIRVMNPTSEAAGLATI
                     TAAHFCDFGFWSDVRYLYGQALTAYHHLGDQHGEVHALWGLGEVERCVGEYGQARTHY
                     TQALVLARQLGYRRGEVDALWGLGQTERLVGEYGQVREYHTQALALARQLGYRRGEAD
                     ALWALGQVERLVGEYGQAREYDSQALILARHLGYRRAETEALRGLGQVERLVGEYGEA
                     QESYSQALALARRLSYRRAEADALRGLGEVERLVGEYGPAREYHTQALTLAQQLGDRR
                     GEADALCGLGYIAADTVKRDQAYEAWWKALE"
BASE COUNT          257 a          464 c          512 g          282 t
ORIGIN      
        1 ctcaagcgct ttccaccagg cctcataggc ctgatcacgt tttacagtat cagcagcgat
       61 gtagcccaat ccgcacaatg cgtcagcttc gccgcgccgg tcaccgagtt gctgcgctag
      121 ggtcaaggcc tgggtgtggt attcgcgggc cgggccgtat tcgccgacga ggcgttcaac
      181 ctcgcccagc ccgcgcagcg cgtcggcttc agcacgtcgg taactgagtc gtcgggccag
      241 ggctaatgcc tgggagtagg actcctgggc ctcgccgtat tcgccgacga ggcgttcaac
      301 ttgacccagt ccccgcagcg cctcggtttc agcacgtcgg taaccgaggt gccgggcaag
      361 gattaaggct tggctgtcgt attcgcgggc ctggccgtat tcgccgacaa ggcgctcaac
      421 ttgacccagc gcccacagcg cgtcggcttc gccgcgccgg taaccgagtt ggcgagcgag
      481 ggccaaggcc tgggtgtggt attcgcggac ttggccgtat tcgccaacga ggcgctccgt
      541 ctggcccagt ccccacagtg cgtcgacttc accgcgtcgg taaccgagtt ggcgagcgag
      601 gactagggcc tgggtgtagt gcgtgcgggc ctggccgtac tcgccgacgc accgctcaac
      661 ttcacctagt ccccacagtg cgtggacttc accgtgttgg tcaccaaggt ggtggtaggc
      721 ggtcagggct tggccgtaga ggtatcggac gtcggaccag aaaccgaagt cgcagaagtg
      781 cgctgccgtg atggtcgcta agcctgcggc ttcgctggtg ggattcatga ctcggatgca
      841 cccgagcagg ttgagccgct caatcgttag ccagctccgc gcttgcgacg catcagtaaa
      901 cgtcagcaca gccctggacg ttgtgccctg agcagagccg tcggtgacat ggggacgcag
      961 caaccggtcg gcctgacggg tgatcgccag gtagctatcg gctaggcgac ccactgccgc
     1021 cgcgtgctgg gttggtggtt cctcagcgtt ggcgcgttct cgggcgtaga ggcgtacaag
     1081 gtcatgcatt cggtatcgcg gaccgcccgg aacgcccgcg agtgagagct gctcgagcag
     1141 gttatggtcc attagttcac gtagcagccg cctgccttgg gcgggtggca cgtcggctac
     1201 tgcagtgatc accactgggg tgatcgcagg cccaggatgc caacccagca gacggaaggc
     1261 ccgacgttgt tcatcggtta agagctggta ggaccacgca aacgccgtgc gtactgaaag
     1321 gtgctcggct accagcgcat ccagcgctgc ggtgtgatcg gccaggtccg cggcgacgtc
     1381 ggtgaggagt tcgtctcggt ggtgacgtag gcgcccggcg atgagccgaa tcgccaaggg
     1441 caaccgagca cacgcggtca ggatctgccg aagcgcgtcg cggtcctcgc agcggtgttg
     1501 gccgaccagc ttgat
//
LOCUS       DATADN010000520         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_373273, whole genome shotgun sequence.
ACCESSION   DATADN010000520 DATADN010000000
VERSION     DATADN010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373273"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VHH53_06755"
     CDS             complement(<1..1140)
                     /locus_tag="VHH53_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX2289883.1"
                     /translation="MTARLVASGFMLLVLAGCGWSRGQDSTPGPPALQAASQLPPAKE
                     AAKSVSRSLWGVVPAAPKRKQDLRPELIKGSAVAVADDALLTSCSVLGNRKQVGLIRH
                     NKYRMAKLVSADPRGEVCVLRVPDGPLNVAAGYRDPADLRLGEPVYALMNQTSSDVAL
                     VQGELTGLAAQEGGPRLETSLVLPADVQSGVLIDGYGNLVGVAAPRPGTAGGVTMTAV
                     PGRLAPQLASLDAPSSTVPLPVAAPRAVPVAATSPPPVLVLQLDDDRDTDRQSIGVAR
                     AAPAAPATEMAGTSTTVDQEDTTSTVSRSEGRTSASRSDNGGRSGRGAGGSTASGATG
                     DTSTDTSDTVTDGSAARSSGTGNAGANTGSIGSSGTGSGTAGAGVD"
     gene            complement(1276..2118)
                     /locus_tag="VHH53_06760"
     CDS             complement(1276..2118)
                     /locus_tag="VHH53_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289884.1"
                     /translation="MRPERSDRLQRLADALEPRFRRARFRRAVHYVCWICEDPRTLGL
                     QRLNLILWYADRNLYLRRRQPLGGATYLRHHPGPRALPLEAQLRELERDGLIARRPRG
                     SGTEADLLFALAPPDLGALDAEEVGELEAAMRGVCLDARATIPHRTAHDTIWRAALIG
                     ETLPYFTVYAGRSGDLYPADLQWAAREVNAGASTGTFRISGAEHPRGGEVIAALLWHL
                     RREPAIGTSLPAVEGSWFVHKQAGIPEASIPEVTVVYRFELDELLPAAIRLGPVPDEL
                     DDET"
BASE COUNT          316 a          805 c          783 g          376 t
ORIGIN      
        1 gtccacgcca gcgcccgcag tgccgctccc ggtgccgctg cttcctatgc tgcccgtatt
       61 cgctccagcg ttcccggtcc cgctgctcct ggcggcgctt ccgtccgtga cggtgtcgct
      121 agtatccgtg ctggtgtctc cagtggctcc actcgccgtg cttccgccag caccgcggcc
      181 gctccggccg ccattgtcgc tgcggctggc gctggtacgg ccctcgctcc ggctcacggt
      241 gctggtggtg tcctcctggt ccacggtcgt gctggtgcca gccatctcgg tcgccggcgc
      301 tgctggcgcc gcccgtgcca ccccaatact ctgccggtcc gtgtcccggt catcgtccag
      361 ctgcagaacc agcaccggcg gtggtgacgt ggctgccacc ggcacggcgc gtggtgctgc
      421 caccggcagc ggcaccgtgg acgaaggcgc atcgaggctc gccagctgcg gtgccagccg
      481 cccgggcacg gccgtcatcg tcaccccgcc ggccgtgccc ggccgtggtg ctgccacacc
      541 cacgagattg ccgtacccgt cgatcagcac cccgctctgc acatccgccg gcaggacgag
      601 gctcgtctcc agccgcggcc caccctcctg cgccgcgagc ccggtgagct cgccctgcac
      661 cagcgccacg tcggagctgg tctggttcat gagcgcatag accggctcgc ccagcctcag
      721 atcggccgga tcgcgatagc ccgccgctac attcagcggc ccgtccggca cccgcagcac
      781 gcagacctca cctcgcgggt cggcggagac gagcttggcc atccggtact tgttgtgccg
      841 gatcagaccc acctgcttcc tgtttcccag caccgagcag ctggtgagaa gcgcatcgtc
      901 cgccaccgca accgcggagc ccttgatcag ctccggccgc aggtcctgct tgcgcttcgg
      961 tgctgccggt accacgcccc acagggaacg cgacacggac ttggccgcct ccttagccgg
     1021 cggcagctgg cttgcagcct gcagggccgg gggaccaggc gtgctgtcct gtcctcggct
     1081 ccagccgcag cccgcaagca ccagcagcat gaaaccgctc gccaccagcc gtgccgtcat
     1141 ggccctcctt cccgaattgt tgagcgcaaa gaagcgcttt ccgctaaacc catttgctcc
     1201 agatcgcccg aagcggcggt gtagtgcagg ttgtggtgac ggtaccgtgt gctccggacc
     1261 gtacttggct tcggctcagg tttcgtcatc gagctcgtct gggacaggtc cgaggcggat
     1321 ggcggcggga aggagttcgt cgagctcgaa gcggtaaacg acggtgacct cggggatgct
     1381 tgcctcgggg atgccggctt gcttgtggac gaaccaggag ccttcgacgg ctggaagcga
     1441 ggtgccgatg gcaggctcgc ggcggagatg ccagaggagc gctgcgatga cttcgccgcc
     1501 gcgcgggtgc tcggccccgc tgatacggaa cgtgccggtg ctggcgccgg cgttgacctc
     1561 gcgggcggcc cattgcaggt ctgctggata gaggtcaccg gatcgtccgg cgtagacagt
     1621 gaagtagggc agggtttcgc cgatcagggc cgcccgccag atcgtatcat gcgcggtgcg
     1681 atgagggatg gttgcgcgtg catcgaggca cacgccgcgc atcgccgcct cgagctcgcc
     1741 tacctcctcg gcatcgagag cgccgaggtc ggggggagct agggcgaaaa gcagatcggc
     1801 ctcggtaccg gagccgcgtg ggcggcgtgc gatcaggccg tctcgctcga gctcgcgcag
     1861 ctgtgcctcg agcggcagcg cccgcgggcc gggatggtgg cgcaggtagg tggcgccgcc
     1921 gagcggctgc ctgcgtcgca ggtacagatt gcggtcggca taccagagga tgaggttcaa
     1981 ccgctgcagg ccgagggttc tcgggtcctc gcagatccag cacacgtaat ggaccgcgcg
     2041 ccggaaccgg gcacggcgga agcgcggctc gagcgcatcc gccaggcgct ggagacgatc
     2101 tgacctctca ggccgcatgg tggccgacct tccctcagtc ccatccgcct tgatcgaacg
     2161 cgcctggggc gagcgccgcc ggcatggcga gcttgaacct tcagccgatc ggggggctcc
     2221 cgtggctgcg gctggaggag cccccgaccg gccctgggcg tctgctaggg agtacccagg
//
LOCUS       DATADN010000521         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1426476, whole genome shotgun sequence.
ACCESSION   DATADN010000521 DATADN010000000
VERSION     DATADN010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426476"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1942)
                     /locus_tag="VHH53_06765"
                     /pseudo
     CDS             complement(350..1942)
                     /locus_tag="VHH53_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
BASE COUNT          465 a          755 c          757 g          432 t
ORIGIN      
        1 ccgaccgccc gacaatgatc accatcctgc gatggcttga cccgcaccag cacccgatga
       61 tctcactggg cgtgggctag cgcggacaac accacaaccg tgccaacaac cgcccaggca
      121 ccgcggtcat cgcgatgacc ctctacggca tccgacggcg cctgtgccgc ccgctagtaa
      181 cgcgaggcgc gccgtcattc tgctgaccgt gtgatgctcg cgcaggctgc atcgtgggcg
      241 ggtggggagc cgcaaccgtg acgggtgatt ttctggggcc taccgtcagt tccaggcact
      301 ggttgagtcc gaatcccacg agagtaagcg atgcggacat ggcacaagat tagccatggc
      361 cgattagcgc tttgccgacg gccgtgagca cggcgttcca caccggtgtc acgttgtcgt
      421 agggcaggtt ggggtgattg cgattggtga gcatcacgat caccaagcct cgctgggggt
      481 ccacggcgaa ctgagtgccg gtgaacccgc tgtggccgat gcctccatcg tcaagaccag
      541 gaatgtcctg gaaccgatgt gtccagaaac cgaggccctg gccccgatgg aattgatcac
      601 gagtgaactc agtaacagta gcttcggcga acaggcgccg agtgccgtag ccaccaccgt
      661 tgatcaaggc ctgaccgtag accgcgacat cgcgggcggt agcgaacacg ccagcatgac
      721 cagcgacacc accaaaggca tacgcactgt tgccgtcgtt gacctcgccg cgcagcgtat
      781 gccgacgcca acccgtgaaa tcgtctacgc ctcggttacc gaggatcgga tacggaacac
      841 cggttgagat catgtcgcgt tcgtaagggt ttccggtcga cgtcgccgcg aagcgctcgg
      901 ctgggtagcg ctcggccggg cgaaagccgg tgtcactcat gcccagcggg ccgtggatct
      961 cgtcccggac gtagtcattg agctgacggc cggtttcgcg ctgaactatc tggccgagca
     1021 gcatgaaccc tagatcggag tagtagcgcg cgtcgcagac cgggtagcgc aatggcaggc
     1081 cggtgacgta ccggatcgcc tcgcggccat tggttgcccc cagataggtt ggctgccact
     1141 gccacattcc ggagcagtgg gtgagtaggt ggcgaacggt gatttcgctc ttgcctccgc
     1201 cggtgaagtc cgaacccagc catcggctga cctagtcatc gaggttgacc cgaccttggt
     1261 cgacgagttt catgagcgcg gccgtggtgg caacgacctt gcttatcgag gccaagtcaa
     1321 agatagtgtc tggccgcatc ggtcgcggcg tcggcagctg ctgggcacca gcgtaggtct
     1381 gagcgttgcc ataggcggtg tgtttgacaa cgacaccatc cttggccacc agaacgaccg
     1441 cgccggggaa aacactgtcc ggatttggtc cgatgtgcgc gttgatcgct tcatcgatgc
     1501 cggccaacgc ctgggcatcc agtccaacgt cttgtgctcg acccgagctc agcgtggcaa
     1561 acggcaccac cgcacgacca ctgccgtgca tggtagtgac atcgatcgtg ttggcaccgc
     1621 gccacaggta aggatccaac gaaatttcct ggcggctggc cagtgctgcg tgtgcagcgg
     1681 ccatgggcag cggtatgccg ttgaccgtca ccacaaccac cgtgcccagc gtggtatcca
     1741 gcaccaaccc agcgcggccc gccggcgcca ggaaggttcc cttggcctga cctccgggca
     1801 ccagatcgac gaccagccgg ccgtcgaggg agtactgggc gtcgttcgct gtggggctcg
     1861 cagacggtgt cccagcggca gtggtgacca tggcgaccgc aataccaatg atcgtccagc
     1921 gggtaccaag tcgagtacgc atcgcggtac cccttcatgc cagcggaagc cgacagccgc
     1981 cgtctacgag actaagaagt cagttcagaa gggttgatgc ccagatggat gtcaagccgt
     2041 cggtgatggt gctcgcgcat gtcaacgaga tagccataga cgatctcggc ggaggcatcc
     2101 accctccgct cggcacagac ataattgtcg ccatacgaca ttgcctatca cggtgagcga
     2161 ccggctccat cgcgcggtca ggcggtcgct tgaaagcgca cgaggcccac tgtgcggcca
     2221 gggtcgcgcg gatgaaggtc aagtcctggt gggcgggctg gccgtcatcc tgatcttgat
     2281 gttgtcgggt ccgggtggca tatctgcacg aagctaagtc gcaccgccgc atcctgagtt
     2341 gactccctgg cgcgctgcca cacccgctga cggcactggc tccacaagac acaccgcaat
     2401 gcactcttt
//
LOCUS       DATADN010000522         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_519869, whole genome shotgun sequence.
ACCESSION   DATADN010000522 DATADN010000000
VERSION     DATADN010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519869"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            572..799
                     /locus_tag="VHH53_06770"
     CDS             572..799
                     /locus_tag="VHH53_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289885.1"
                     /translation="MATRKVTLSIDHTAWALAEAAAARAGVSPSAWLSAAARREAVRL
                     GAGTDWGDAEAEALAYDGDLAAAEADIRAAG"
     gene            855..1085
                     /locus_tag="VHH53_06775"
     CDS             855..1085
                     /locus_tag="VHH53_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289886.1"
                     /translation="MSSDGINESPRPWLLGIELRDRDPGDILAVPVADHGWAYAGDLS
                     KLYRAWTGDQLGVLDSATMTRLDGALRAALDL"
     gene            complement(1106..1927)
                     /locus_tag="VHH53_06780"
     CDS             complement(1106..1927)
                     /locus_tag="VHH53_06780"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2289887.1"
                     /translation="MADERLSDPRSALAAKIRFDVPHAARIWNYWMGGKDNYDADRAA
                     GDAVAEVYPDIVTMAVQSRQFLIRAVRYLAGEAGIHQFLDIGTGLPTMENTHEVAQKV
                     APESRIVYVDNDPLVLVHARALLVNTTPEGVTTYVDADVHDPELIISDARNVLNFSQP
                     IAVMLMGVLGYVADFDEARSIVARVMAAVPSGSYLVLWDGTKTGDAIVEGGAKLAESG
                     AIPYNLRSLEQISQCFEGLQLVDPGLVPITQWRPDTAEVGKAEPIDAYGAVARKP"
BASE COUNT          370 a          675 c          693 g          373 t
ORIGIN      
        1 cgcgtagact ccgccataaa gatcggcacc agccgaaatg gttgagcgat ctttctcgtc
       61 tggtacgcac tcacatgccg ctgatcgtgc atggctacca gcgctaacca tgtctagcgg
      121 ttcgtaagcg gaaccccgca gcgacgtcag taatccagag gtcaaagggt ggaaccggat
      181 cgataaaacc acgttgttgc gcacgatcat cggtcagctt gccgccggtc ggggaagggt
      241 gttcacgtgg gcagcaggtg cgagcctaga gccgtcggcg tcgggaacgc ccgaatagct
      301 gtcccaggac cctgaacggc agcgttacga cgctgatcaa ggtgctgatg atggcaccga
      361 tggcgccctt gcctcggcgg actggctggg tcatggcgta cctcctacga gcctgcctgc
      421 atcgggacta cccgtttggg ttgaagtcca cacagatgcc gtgagccgac ggccgcgcca
      481 gatgttcggc ggcaccggcg aggccatgta ggtcagatgt caccgggccg tatccacgtg
      541 caccggtgcg ccgattggta cgataaaggg tatggccacg cgtaaggtca ctttgtcgat
      601 cgaccacact gcatgggcgc ttgccgaggc tgcggccgcc cgggcgggcg tctcgccctc
      661 ggcctggctg tcggctgccg ctcggcgcga ggccgtccgc cttggcgccg gcaccgactg
      721 gggcgacgcc gaagccgagg ccttggctta tgacggcgac ctggctgcag ccgaggcgga
      781 catccgtgcg gcggggtgaa ctacgtcgtt acgtccgacc aagcacccct gaacgtctgc
      841 ggacggtcgc gatcatgtcc agcgacggca tcaacgaatc gccgcgcccc tggctgctgg
      901 gcatagaact ccgtgaccgc gaccccggcg acatcctcgc cgtcccggtg gcagatcacg
      961 gatgggccta cgcaggcgac ctctccaagc tttaccgggc ttggacgggt gatcaattgg
     1021 gcgtgctgga ctcggcaacg atgacgcgcc tcgacggtgc gctacgcgcc gctctggacc
     1081 tgtaggtgcg cgcgatgctc gctcctcacg gcttgcgcgc cacggcgccg taggcatcaa
     1141 tcggttcagc cttgccgacc tcggcggtgt ccggccgcca ctgcgtgatc ggcaccagac
     1201 cggggtccac cagctgcaag ccctcgaagc actggctgat ctgctcgagg ctacgcaggt
     1261 tgtaagggat ggctccgctt tccgctaact tcgcgccgcc ctcgacgatg gcgtccccgg
     1321 ttttggtgcc gtcccacagc accaggtagc tgccggacgg gacggcggcc atcacccgcg
     1381 ccacgatgga ccgtgcctcg tcgaagtcgg cgacgtagcc cagcacgccc attaacatga
     1441 ccgcaatcgg ctggctgaag ttcagcacgt tgcgggcatc ggagatgatc aactctgggt
     1501 cgtgaacgtc tgcgtccacg taggtggtca ccccctcagg ggtggtgttg accagcaggg
     1561 cgcgggcatg caccagcacc agcgggtcgt tatcgacgta gacgatccgg gactcgggcg
     1621 caaccttctg ggcgacctcg tgggtgttct ccatcgtggg caggcctgtg ccgatgtcga
     1681 gaaactgatg tatcccggcc tcaccggcca ggtaacgcac agcgcgaatg aggaactgcc
     1741 gggactgcac ggccatagtg acgatgtccg ggtacacctc ggcgacggca tcaccagcgg
     1801 cacggtccgc atcgtaattg tccttgccgc ccatccagta attccagatc cgtgccgcat
     1861 gcggcacatc aaatcggatc ttggcggcga gcgcggagcg cgggtcggac aaccgctcat
     1921 ctgccacgtt gagctcctat gtacggggtt gcaggcaaga tggtggcgag atcgtaggct
     1981 ggacccgggc caccaggaac ctgccaacga ttccgctcga tatgatggca cgagcctgct
     2041 tacccgctgg cgaggtgcga gtgaaatggc gcgtggcgcc actgtcacag ggacttgctg
     2101 gtcggcgagc c
//
LOCUS       DATADN010000523         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1426484, whole genome shotgun sequence.
ACCESSION   DATADN010000523 DATADN010000000
VERSION     DATADN010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426484"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VHH53_06785"
     CDS             <1..995
                     /locus_tag="VHH53_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2289888.1"
                     /translation="DAVPAVLAASVPDNCARFAVSRLPGSGTVRLSYSLHLVERGIHT
                     LGPLVARIADPLGLAEYRCTLAGSDRLVVTPVVTALTGMPAGGELGTGAVGAGRVGAG
                     TGQDAVVVRSYRQGDDLRQVHWRTTARRDELMVRVQEWPWRGGVTVLLDHRAAAHRGN
                     GPTSSVEYAVSLAASVYVHLVRHGQRVRLITEEGVVHAGGTDVTAQTIDTALEALAAL
                     RTRYQPDFTSGPALAAGQEVIAVLGAVGPAVVEQLLAHRPRGMRSHAVLLDVAAWRVA
                     GDVSTAAVGADPTQAARLLVAAGWSVTVARPEQPPSVVWNHLCISSRSKWEALR"
     gene            992..>2096
                     /locus_tag="VHH53_06790"
     CDS             992..>2096
                     /locus_tag="VHH53_06790"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HEX2289889.1"
                     /translation="MNVLSWFAARRLTGPATGAVGGLAVALAATSLTGLLTGARWWGL
                     VVVTTAAIVAAGVLLRLLRTPPIVVAAGQLAVLAGLVTALFTGSGRLLVLPGPDAVVE
                     LASLLGRAAQQVRDGIPPVPESTELICLLVVAFGLVAIFVDTLAVSAAAPATTGLVLV
                     GVIIVPAALSDRLLPWWSFALAALAFALLLAINNAARPTTRHHLVWTDSASPGWHVGA
                     APAAVTVSVGAMAVALLVGTSVSAVGTGQPGTGNGGQISGIGLNPFTSLRGQLNAGEV
                     VDLFRVRGLNQRAYLRALTLSRFDTQAGWLQGPLDGAVPAGGGQQPAGRLPLPTGVSQ
                     PVPGSLVQVQIEPINYVDNWLPSYGYPLALEGIG"
BASE COUNT          273 a          698 c          771 g          354 t
ORIGIN      
        1 aggatgcggt gcccgcggtg ttggccgcct cggtaccgga caactgtgct cggtttgcgg
       61 tgtcgcgcct gccggggtcc ggcacggtac ggctgagcta ctcactacac ctggtggaac
      121 gcgggataca caccctcggc ccactggtgg cacggatcgc cgacccgttg gggttggccg
      181 agtaccgctg cacgctcgct ggaagcgacc ggctggtcgt gacgcccgtc gtgacggcgc
      241 tgaccgggat gcccgctggg ggtgagctgg gcaccggcgc tgtaggcgcc gggcgggtgg
      301 gcgccgggac tggccaggac gccgtggtgg tgcgcagcta ccgtcaaggc gatgaccttc
      361 gccaggtgca ctggcgcacg acggcccggc gagacgaatt gatggtgcgc gtgcaggagt
      421 ggccctggcg cggcggtgtc accgtgctgc tggaccatcg cgccgcggca caccgcggca
      481 acgggcccac ctccagtgtg gagtacgcgg tctccctagc cgccagtgtg tatgtgcacc
      541 tggtgcggca cgggcagcga gtacggctga tcactgagga gggcgtggtg cacgccggtg
      601 gtaccgacgt gaccgctcag accattgaca ccgcgctcga agccctcgcc gcgctgcgca
      661 cgaggtacca gcctgacttc accagcggtc cggcgctggc cgcgggtcag gaggtgatcg
      721 cggtgctcgg ggcggtcggg ccggcagtcg tcgagcagct gctggcgcac cgcccacgcg
      781 ggatgcgcag ccacgccgtg ctgctcgacg tcgcagcgtg gagggttgcg ggggacgtct
      841 ccactgctgc tgtcggggct gatccgacgc aagccgcccg cctgctcgtc gcagcgggct
      901 ggtcggtcac cgtggcccgt ccagaacaac caccgtcggt ggtgtggaat cacctgtgca
      961 tcagttctcg cagtaagtgg gaggcgctgc ggtgaacgtc ctcagctggt tcgctgcccg
     1021 gcgcctgacc ggcccagcaa ccggcgcagt gggtgggctg gccgttgcgc tcgccgcgac
     1081 gtcattgacc ggtctgctca ccggggcgcg ctggtggggc ttggtggtgg tgaccaccgc
     1141 agcgatagtc gccgccggag tactgctgcg gttgctgcgg acgccgccaa tcgtggtcgc
     1201 ggccggtcaa ctcgccgtgt tggccgggtt ggtcaccgcg ctgttcacgg gcagcggacg
     1261 gctcctcgtg cttcccggcc cagacgccgt cgtcgagctg gcatcgttac tcgggcgcgc
     1321 cgcccagcag gttcgggacg gcatacctcc ggtgccggaa agcaccgaac tgatctgcct
     1381 gctggttgtc gccttcgggc tggtcgccat tttcgtggac accctggccg tctcggccgc
     1441 cgcgcccgcc actacggggt tggtgctggt gggcgtcatc atcgtacctg ccgcgctgtc
     1501 agaccggctg ctgccctggt ggagcttcgc actcgccgca ctggctttcg cgctgctgct
     1561 agccatcaat aacgcggcac ggcccactac gcgccaccat ctggtgtgga ccgactcagc
     1621 cagtccaggg tggcatgtcg gtgcggcgcc cgctgcggtc accgtgagtg tcggtgccat
     1681 ggcggtcgca ctgctcgtcg gaacgtcggt cagcgccgtg ggcaccggtc agccaggcac
     1741 tggcaacggc gggcagatca gcgggatcgg gttgaacccc ttcacctcac tgcgcggcca
     1801 gctcaacgct ggagaagtcg tggacctgtt ccgggtccga ggcctgaacc agcgcgccta
     1861 cctgcgggcg ttgaccctga gccggttcga cacgcaggcg ggctggctgc agggtccgct
     1921 ggatggcgcg gtaccggcgg gtgggggcca gcagccggcc gggcgcttgc cgctgcccac
     1981 cggggtgagc cagccggtcc ccggatccct ggtgcaggtg cagatcgaac cgatcaacta
     2041 cgtggacaat tggctgccct catacggtta cccgctggcc ttggagggca tcggcc
//
LOCUS       DATADN010000524         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1126358, whole genome shotgun sequence.
ACCESSION   DATADN010000524 DATADN010000000
VERSION     DATADN010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126358"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VHH53_06795"
     CDS             complement(<1..191)
                     /locus_tag="VHH53_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX2289890.1"
                     /translation="MVTPVTPTGPRTWPVLLTQLITGTDLDADDTAWAMDQVMTGAAT
                     PAQVAAFVVALRGKGETSA"
     gene            complement(202..618)
                     /locus_tag="VHH53_06800"
     CDS             complement(202..618)
                     /locus_tag="VHH53_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289891.1"
                     /translation="MTAPSGATTVLVFSHRPEVREAIINAVGRRPAPDVGRLNYVEAG
                     TIAEVLAQVDGGAVNLAILDGEAQPTGGMGLSRQLKNEIAQCPPVIVSVRRRDDRWLA
                     VWSQADAVIVYPLDPLVAAETVAEVLRSHRLPAANG"
     gene            823..1461
                     /locus_tag="VHH53_06805"
     CDS             823..1461
                     /locus_tag="VHH53_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX2289892.1"
                     /translation="MLGVTSSSSLAAPSISQRVHSLNRPNMVSVGTIVWLSSELMFFA
                     GLFAMFFTVKANNDGPWPPILESTGHPIHLDVPYALVVTIILVASSFTCQWGVFKAEQ
                     GDVFGLRLWYTITLIMGTLFVLGQANEYRNLIHEGVTIPSGAYGTVFYLTTGFHGLHV
                     TGGLIAFIFLIVRTKLSKFTPAQATAAIVVSYYWHFVDVVWIGLFATIYFLP"
     gene            1642..2382
                     /locus_tag="VHH53_06810"
     CDS             1642..2382
                     /locus_tag="VHH53_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX2289893.1"
                     /translation="MLALAVALLSAGGLYTVLAPQPQIAHAQEDPALVRQGEQLYNNA
                     CISCHGANLQGVTDRGPSLICTGEAAVYFQVSTGRMPAVRQEAQILEKPVKYTAEEID
                     ALGAFVQANGGGPTVPPAGSLAGGDPAAGGEMFRLNCASCHQFTGRGIALSSGKFAPA
                     IEGVNPQQIYTAITTGPQSMPKFSDRQLTPEDKRDIIAYLESVTENRNNPGGYALGGY
                     GPFSEGLVAWIVGMSALVAVTLWIGAKA"
     gene            2379..3500
                     /locus_tag="VHH53_06815"
     CDS             2379..3500
                     /locus_tag="VHH53_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX2289894.1"
                     /translation="MSGPGEQGQRPGDPDLSTSSRAELVRQGLAADGVTLAHQEDPFP
                     VQGTRAEKRTERKVAWWFIIAALAGLGFLAAYLFWPWEYVAPGSPNSQHLIYQLYTPI
                     IGLLLGMSVFAIGAGTIAYAKSLLPHETAVQQRHMGASAEVDRVTTTAILTDTGARSH
                     IGRRSLIKRAAGLGAGVMGVGIGVLALGGLVRNPWQGGDEAALWVTPWRSDDGEPVFL
                     RYEDHEITLARPEDLAAGSIATVFPYKRSWSEEEAHEALRASDSPVMLIRLRPEQASQ
                     VVQRQGQVDFHYGDFYAYSKICTHLGCPASLYEAQTARLLCPCHQSQFDMLAYAKPIF
                     GPATRALPQLPIDVNDEGYFYATGAFIEPVGPAFWERRS"
     gene            3506..>4758
                     /locus_tag="VHH53_06820"
     CDS             3506..>4758
                     /locus_tag="VHH53_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEX2289895.1"
                     /translation="MTTPSKSSGLAAKAGKTADELDQRFHLAAGMRRQINKVFPTHWS
                     FMLGEIALNTFIVLLLSGTYLALFFDPSMAEVEYQGPFENLRGIEMSRAYASTLELSF
                     EVRGGLFIRQVHHWAALLFVAAIVAHMFRTFFTGAFRKPRETNWMIGVVIALLAGLEG
                     LTGYSLPDDLLSGTGLRIISAITMSPPVIGTWLHWAIFGGEFPGTVIIPRFYIIHVLI
                     LPGILFALIAVHLALVWYQKHTQFPGPGRTERNVVGVRILPIFAVKAGGFAMVNIGVI
                     CIMAGLFQINPVWNFGPYNPAQVSAASQPDWYVGWLDGIARVWPPWEIYLGDYMIPQN
                     FWPTMVLPAIMFTLAATYPAIERKLTNDNASHNLLQRPRDVPVRTALGMMAISFMFVL
                     LLSGGNDVIAYVLNNSLNATTWAGR"
BASE COUNT          792 a         1617 c         1491 g          858 t
ORIGIN      
        1 tccgccgagg tctcgccctt gcctcgtaac gccaccacga acgccgccac ctgcgcgggc
       61 gtcgccgcac cggtcatcac ctggtccatc gcccatgcgg tgtcatcggc atccagatcg
      121 gtcccggtga tcaactgagt aaggagcaca ggccaagtac ggggaccggt cggcgtcact
      181 ggcgttacca tggggggcgc gtcaaccgtt cgctgccggc agccggtgcg agcgaagcac
      241 ctcggcaacc gtctccgcgg cgaccagcgg atccagcggg tacacgatca ccgcgtcggc
      301 ttgtgaccag acagccaacc agcggtcatc cctacgccgc accgaaacga tcactggcgg
      361 gcactgcgca atctcattct tgagctggcg ggacagcccc atccctcccg tgggctgcgc
      421 ctcaccgtca aggattgcca ggtttaccgc acccccgtcg acctgtgcta acacctcggc
      481 aatcgtcccc gcctcgacgt aattcagccg gccgacatcc ggcgcgggac gccgcccgac
      541 cgcgttgatg atggcctcgc gcacctccgg tcggtggctg aagaccagaa cagtggtggc
      601 gccgctcgga gcggtcatct ggtaatcatc tgatggtcaa ggtcctcatc tcagtgcgcc
      661 cgcggccggg cagggccccg tggccggctc gggttcatgg cccggctcat gggcagggtc
      721 accgcgcggg cggtggcgtt gatgatatcc agccaaggtt cgtcgctccg ggcaggcgcc
      781 cggctggccc cggccccgcc gaggtaggcg ccgttggcca taatgcttgg tgtgacctcc
      841 tcgtccagcc tggcagcgcc ctctatcagt caacgggtgc actcgctgaa ccggccgaac
      901 atggtcagcg tcggcaccat cgtatggctg tccagcgagc tgatgttctt cgccggcctg
      961 ttcgcgatgt tcttcaccgt caaggcgaat aacgatggtc cgtggcctcc gatcctcgag
     1021 tcgaccggac acccgatcca tctcgacgtg ccctatgcgc tggtggtcac gatcattctg
     1081 gtggcctcgt cgttcacctg ccagtggggc gtcttcaagg ccgagcaggg cgacgtgttc
     1141 gggttacggc tctggtacac gatcacgcta atcatgggca cgctgtttgt gcttggccag
     1201 gccaacgaat accgcaacct cattcacgag ggcgtcacga tcccctccgg tgcctacggc
     1261 acggtgttct acctcactac cggcttccac ggcttgcacg tgaccggcgg tttgatcgca
     1321 ttcatcttcc tcatcgtccg gaccaagctg agcaagttca ctccggcaca ggccaccgcc
     1381 gccatcgtcg tgtcgtatta ctggcatttc gtcgacgtgg tgtggatcgg cctgttcgcc
     1441 accatctact tcttgcctta gcaccagcac ccagccgaga agacagcaga gccgaaccag
     1501 acagcagggg tagccgagac gatgaccacc gcaaactccg acgcacgatc gagccctgcc
     1561 gacggagcga accaccagga atcggactca gcccgatcgc ggcggggagg ccgcggccgg
     1621 ctgcgccgtc gggtgtccgg gatgctggca ctcgccgtgg cactgctgtc tgccggcggg
     1681 ctgtacacgg tgctggcacc gcagccgcaa atcgcgcacg cccaagaaga tcctgcgctg
     1741 gtgcgccagg gcgagcagct ctacaacaac gcctgcatca gctgccacgg tgccaacctg
     1801 caaggagtga ccgaccgagg acccagcctg atctgcaccg gagaggctgc ggtgtacttc
     1861 caggtctcca ccggacggat gcccgcggtg cgccaggagg cgcagatcct tgagaagccg
     1921 gtcaagtaca ccgcggagga gatcgacgcg ttgggcgcct tcgtgcaggc caacggcggc
     1981 ggtccgacag tgccgccggc aggaagcctt gccggcggtg acccggctgc gggtggcgag
     2041 atgttccggc tcaactgcgc gtcctgccac cagttcaccg gacgcggcat cgcgctgtcc
     2101 tccggcaagt tcgctccagc cattgagggc gtgaatcccc agcagattta caccgcgata
     2161 accaccgggc cgcagagcat gcccaagttc tccgaccggc agctcacccc agaggacaag
     2221 cgcgacatca ttgcctacct cgaatcggtc accgagaacc gcaacaaccc gggtggttac
     2281 gccctcggtg gctacgggcc gttctcggag ggcttggtcg cctggatcgt cgggatgtcc
     2341 gcgctggtcg ccgtcacgct gtggatcgga gccaaggcat gagcggcccg ggcgagcagg
     2401 gacagcgccc gggtgacccc gacctgtcca cgtcgagccg cgcagaactg gtccggcagg
     2461 gcttggccgc cgacggggtg accctcgctc accaggagga cccgttcccg gtgcagggca
     2521 cccgtgccga gaagcgcacc gaacgcaagg tcgcgtggtg gttcatcatc gccgcgctgg
     2581 ccgggctggg gttcctcgcg gcctacctgt tctggccgtg ggaatacgtt gcacccggca
     2641 gcccgaacag ccagcacctg atctaccagc tctacacgcc gatcatcggg ctgttgctcg
     2701 gtatgtcggt gttcgccatc ggcgccggga ccatcgccta cgccaagagc ctgctgccgc
     2761 acgagaccgc ggtccagcaa cgtcacatgg gcgcttcggc agaggtggac cgcgtgacca
     2821 cgaccgcgat cctgactgac accggcgccc gcagccacat cgggcgacga tccctgatca
     2881 agcgagcggc tggtctaggc gccggtgtca tgggtgtcgg gataggagtt ctcgccttgg
     2941 gtgggctggt gcgcaacccg tggcagggcg gtgacgaagc cgcactgtgg gtcacgccct
     3001 ggcgctcgga cgacggtgag ccggtgttcc tgcgctacga ggatcatgag atcaccctgg
     3061 cacgccccga ggacctcgcc gctgggagca tcgcgacggt cttcccgtac aagcgctcct
     3121 ggtcagagga ggaagcgcac gaggcgctgc gcgcttccga cagccctgtg atgctcatcc
     3181 ggttgcggcc cgagcaggct tcccaggtgg tccaacgcca gggacaggtc gactttcact
     3241 acggcgactt ctacgcctac tccaagatct gcacccatct cggctgcccc gcctcgctgt
     3301 acgaagcgca gaccgcacgg ctgttgtgtc catgccacca atcgcagttc gacatgctcg
     3361 cctatgccaa gccgatcttc ggcccggcca ccagggcctt gccccagctg cccatcgacg
     3421 tcaacgatga gggatacttc tatgccacgg gtgccttcat cgagcccgta ggaccggcgt
     3481 tctgggagcg gaggtcgtaa gtgccatgac gacgccgagc aagtcgtcgg gcctggccgc
     3541 caaagccggc aagacagccg acgagctgga ccagcggttt catctcgctg ccggcatgcg
     3601 ccggcagatc aacaaggtct tcccgacgca ctggtcgttc atgctcggtg aaatcgcgct
     3661 gaacaccttc atcgtgctgt tgctgtccgg gacgtacctg gcgctgttct tcgacccgtc
     3721 gatggcggag gtggagtacc agggtccctt cgagaacctt cgtgggatcg agatgtcccg
     3781 ggcgtacgcg tcgactctgg agctctcttt cgaggtccgc ggcgggttgt tcatccggca
     3841 ggtgcaccac tgggcagcgc tgctgttcgt cgcggccatc gtcgcgcaca tgttccgaac
     3901 gtttttcacc ggcgccttcc gcaagccgcg ggagacgaac tggatgatcg gcgtggtcat
     3961 cgcgctcctg gctgggctcg agggactgac cggctactcg ttgcccgacg acctgctttc
     4021 gggaaccggg ctccggatca tctcggcgat caccatgtct ccaccggtga tcggtacctg
     4081 gctgcactgg gcgatcttcg gtggggagtt ccccgggacg gtgatcatac cccggttcta
     4141 catcatccac gtgctgatcc tgcccggcat cctgttcgcg ctcatcgcgg tgcaccttgc
     4201 gctggtctgg taccagaaac acacccagtt ccccggcccc ggtcgcaccg agcgaaacgt
     4261 ggtcggggta cggatcctgc cgatcttcgc ggtgaaggcc ggcggcttcg ccatggtgaa
     4321 catcggtgtg atctgcatca tggcggggct gttccagatc aacccggtgt ggaatttcgg
     4381 cccttacaac cccgcccaag tctcggcggc ctcgcagccc gactggtacg tcggctggct
     4441 ggacggcatt gctcgggtgt ggcctccctg ggagatctac ctgggcgact acatgattcc
     4501 ccagaatttc tggccgacca tggtgcttcc cgccatcatg ttcaccctgg cggcgacgta
     4561 ccctgccatc gagcgcaagc tgaccaacga caacgccagc cataacctcc tgcagcgccc
     4621 acgcgacgtc ccggtgcgca ccgcgctggg catgatggcg atctcattca tgttcgtgct
     4681 gctgctttcc ggcggcaatg acgtcatcgc ctacgtgctc aataattcat tgaacgcgac
     4741 tacctgggct ggccgcat
//
LOCUS       DATADN010000525         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_667320, whole genome shotgun sequence.
ACCESSION   DATADN010000525 DATADN010000000
VERSION     DATADN010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667320"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..>1711)
                     /locus_tag="VHH53_06825"
     CDS             complement(51..>1711)
                     /locus_tag="VHH53_06825"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEX2289896.1"
                     /translation="YGWGANDIGGSQDYVNRGLYNYRPIYTTATTAANNHVTDNYIHD
                     LMQQMTDGGCLYTLSASPGSTFERNYCHSNNGWFGFYHDEGSRDFTDTNNVFRNTGEW
                     GHEISNATNNTGGLTLTDNWTNNSSANITNTNGRGDVVSGTVVVSDGNWPSAAKTVMN
                     NAGIQPLYRPLTTDPVSSPYSAYSSIPANTGQHGGHFTITDAGKDIWGAGGQHEDEYG
                     TVFRNGAAVDGTSVTARVDNLDKTSGWAKAGVVLRNDLTDDGSSAGYAAVVVTPNNGV
                     SFQRDSDADGYLDKLTSTAATVKAPVWLRLTRTATQVSAYYSTDGSTFTQVGSTVTLP
                     SMATTQDAGVIHTAHSTTAGSATFSNLRIVTSPYKAHSSIPAAVSQSGGVTSLTSAGI
                     DVWRSGTAYDDEYSAAYRTGAAGTSSTVTVRVASQDKTNSWAKAGLMLRNNVASAGSS
                     TGYLVLATTPGNGIALSSDSNGDGYLDTNTTKTAGATVAPVWLRLVRSGTSVTGSYSA
                     DGTAWTTVGTATLTGANSTLDAGMFSTAHAGSIGTATFSRFSVS"
BASE COUNT          230 a          477 c          661 g          343 t
ORIGIN      
        1 cgcaccggca cgcggagctc atctcggaga tggtgacccg aaccgcttcg tcagctgacg
       61 gagaaccggc tgaatgtcgc ggtgccgata cttccggcgt gggctgtgga gaacatgccc
      121 gcgtccaggg tgctgttcgc cccggtcagc gtcgcagtgc cgacagtggt ccaggccgtg
      181 ccgtcggcgg agtaggagcc ggtgaccgac gtgccgctcc tgaccaggcg gagccagaca
      241 ggggcgacgg tggcgcctgc ggtcttggtg gtgttggtgt cgaggtagcc gtcaccgttg
      301 gagtccgacg acagggcgat tccgttgccg ggggtggtgg cgaggacgag gtatccggtg
      361 gacgagccgg cggaggcgac gttgttccgc agcatcaggc cggctttggc ccagctgttg
      421 gtcttgtcct gactggcgac gcggacggtg acggtcgagg acgtgccggc ggcccccgtc
      481 cggtaggcgg cggagtactc gtcgtcgtat gccgtgccgg agcgccatac gtcgatgccc
      541 gcgctggtca gggaggtcac tccgccgctc tggctgacgg cggccggaat cgaactgtgt
      601 gccttgtagg gggaggtgac gatgcgcagg ttgctgaagg tggcgctgcc ggcggtggtg
      661 ctgtgggctg tgtggatgac accggcatcc tgggtggtcg ccatggacgg cagggtcacc
      721 gtcgagccga cctgagtgaa ggtggatccg tcggtggagt agtaggcgga cacctgggtg
      781 gcagtgcggg tgagccgcaa ccagaccggt gccttgacgg tggcagcggt ggacgtgagc
      841 ttgtcgaggt agccgtcggc gtcggagtcc cgctggaagc tgacgccgtt gttcggggtc
      901 accaccacag ccgcgtatcc ggcggaggag ccgtcgtccg tgaggtcgtt gcgcaggacg
      961 acacccgcct tggcccagcc gctggtcttg tccagattgt cgacccgggc ggtcaccgac
     1021 gtgccgtcga cggctgcccc gtttcggaag acggtgccgt actcgtcttc gtgctgtccg
     1081 ccggcacccc agatgtcctt gcccgcgtca gtgatcgtga agtggccgcc gtgctggccg
     1141 gtgttggcgg ggatggatga gtaggcgctg tagggggagc tgacagggtc ggtggtcagc
     1201 ggacggtaca ggggctggat accggcgttg ttcatgaccg tcttggccgc tgacggccag
     1261 ttgccgtcgc tgacgacgac ggtgccgctg accacgtcac cgcggccgtt ggtgttggtg
     1321 atgttcgcgg aactgttgtt cgtccagttg tcggtcaggg tcaggccgcc ggtgttgttg
     1381 gtcgcgttgc tgatctcatg gccccattcg ccggtgttcc ggaagacgtt gttggtgtcc
     1441 gtgaagtccc gggagccctc gtcgtggtag aagccgaacc aaccattgtt gctgtggcag
     1501 tagttgcgtt cgaaggtgct gcccggtgac gcagacaggg tgtacaggca gccgccgtcg
     1561 gtcatctgct gcatgaggtc gtggatgtag ttgtcggtga cgtggttgtt cgcagcggtg
     1621 gtcgcggtcg tgtagatggg ccggtagttg tacaggcccc ggttgacgta gtcctggctg
     1681 ccgccgatgt cgttggcacc ccagccgtag c
//
LOCUS       DATADN010000526         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_48315, whole genome shotgun sequence.
ACCESSION   DATADN010000526 DATADN010000000
VERSION     DATADN010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48315"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2052)
                     /locus_tag="VHH53_06830"
     CDS             complement(<1..>2052)
                     /locus_tag="VHH53_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2289897.1"
                     /translation="WATDNLLSYLREQREAAGHVPDDRTVLVERFRDELGDWRMVVHS
                     PFGAQVNGPWALAIGARLRERRGVEAQVASADDGIVLRLPDALDAEGAEVLPTAEDVL
                     LDPEEVERLVIAEVGGSALFASRFRECAARSLLLPRRDPKRRTPLWQQRQRSAQLLTV
                     ASKYEQFPVVLEAMRECLQDVYDLAGLRELMSEVRARRVRVVEVATPSPSPFARSLLF
                     GYVGMFLYELDAPLAERRAAALSLDSTLLTELLGSEAIRELLDPEVLIEVEAQLQRVD
                     ADRQVRDVEGAADLLRVLGDLTEVEAAQRGVAPEWLAELGAARRAIAVRIAGEQRWIA
                     IEDAGRVRDALGAALPVGVPEAFTEPVADPLGDLMLRYARTHGPFTAAAIGKRFGLGI
                     AVAGTVLDRMTGAGILVRGELRPVQVGGGGTEYCHADVLRRLRRASLARLRAEVEPVE
                     QAALGRFLPAWHGIGPCDTVRKGAGPARLLSSAPTPDDVLGAVEQLAGAPVPASALES
                     LLLPARLPGYSPALLDELTTSGEVTWTGCGSLAGGDGWIALAPTDVADLVLPDVEAQL
                     ELTPTHRGVLGALGWDPAGGPAVGGALFFRQLADRAGQYLRADDEPVPSDADLVMALW
                     DLVWAGLLTNDSLAPLRAVVSGRSLAHRPRPSRPLRRYARMRVRRPPMPSRAGPPTAA
                     G"
BASE COUNT          350 a          758 c          665 g          279 t
ORIGIN      
        1 ggcccgcagc tgttggcggg cccgcccggg agggcatcgg tggccgcctc acccgcatcc
       61 gagcgtaccg gcggagtggt ctggatggac gcggtcgatg cgcgagcgag cgcccagaca
      121 ccacggctcg aagcggagcc agcgaatcat tggtcagcaa tccggcccat accagatccc
      181 acagcgccat gacgagatcg gcgtcactgg gcaccggttc gtcgtcggcg cgcaggtact
      241 ggccagcccg gtcggcgagc tgcctgaaga acagggcacc gcccacggca ggaccaccag
      301 ccggatccca acctagtgcg cccagaacgc cacggtgtgt cggtgtgagc tcgagctggg
      361 cctcgacgtc cggcagcacg agatccgcta cgtcggtggg agccagcgcg atccacccgt
      421 cgccgcctgc caacgatccg cagccggtcc acgtcacctc accggatgtg gtgagttcgt
      481 caagcagggc aggagagtag cccggcagcc gggccgggag cagcagagat tccagtgcgc
      541 tagccggcac gggtgcgccg gctagctgct cgactgcgcc gagcacatcg tcgggagtgg
      601 gcgcgctaga cagcagccgc gccggtcccg cgccttttcg cacggtgtcg cagggtccga
      661 tgccgtgcca agccggtagg aaccgcccca gcgccgcctg ctcaactggc tccacctcgg
      721 cccgtaaccg ggccagcgat gcccgtcgca gtcgccgtaa cacgtcggcg tggcagtatt
      781 cggtgcctcc accaccaacc tgaaccggcc gtagttcgcc ccggacgagg atgccggccc
      841 cggtcatccg atccagcacc gttcccgcca ccgcgatgcc aagcccgaac cgtttcccaa
      901 tcgccgcggc ggtgaacggt ccatgagtgc gggcgtagcg cagcatgaga tccccaagtg
      961 gatcggcaac cggctctgtg aaagcttcag gcacccccac cggcagggcc gcacccagcg
     1021 cgtcacgtac ccggccggcg tcctcgatag cgatccagcg ttgctcaccg gcaatccgca
     1081 ccgcgatcgc tcgacgggcc gcaccgagct cggcgagcca ttccggcgcg accccgcgct
     1141 gcgctgcctc gacctcggta aggtcaccca gcacccgcag caggtctgca gcgccctcga
     1201 cgtctcggac ttgccggtcg gcatcgacgc gctgcagctg tgcctcgact tcgatgagca
     1261 cttcggggtc gagcagctcg cggatcgcct ctgagccgag caattcagtc agcagcgtgg
     1321 agtcgagcga cagcgccgcg gcccggcgct cggcgagcgg ggcatcgagc tcatagagga
     1381 acatcccgac gtaaccgaac agcaggctgc gcgcaaacgg tgacggcgac ggcgtggcca
     1441 cctcaacaac ccgcacgcgg cgcgcgcgta cctccgacat cagctcgcgc agtccggcca
     1501 ggtcatagac gtcttgcagg cactcccgca tggcctccag cacgaccggg aattgctcgt
     1561 acttcgacgc gacggttagc agctgggccg agcgctgccg ctgctgccac agcggcgtac
     1621 ggcgcttggg gtcgcgccgg ggcagcaaca gggagcgtgc ggcacactcc cggaaccggg
     1681 aggcgaacag cgccgaaccg cccacctcgg cgatcaccaa ccgctccacc tcttctgggt
     1741 cgagcagcac gtcttccgcg gtgggtaaca cctcggcacc ctcagcgtcc agcgcgtccg
     1801 gcaaccgcag cacgataccg tcatcggcgc tggccacctg cgcttcgacg ccgcgccgtt
     1861 cccgtagccg ggctccgatc gccaacgccc acggcccatt gacctgcgcg ccgaatggtg
     1921 aatgcaccac catccgccaa tcgcccagct cgtcgcggaa gcgttccacc aagacggtgc
     1981 ggtcgtcggg cacgtgccca gcggcctcgc gctgctcgcg taggtaggac aacaggttgt
     2041 cagtcgccca gg
//
LOCUS       DATADN010000527         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1257620, whole genome shotgun sequence.
ACCESSION   DATADN010000527 DATADN010000000
VERSION     DATADN010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257620"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1766)
                     /locus_tag="VHH53_06835"
     CDS             complement(<1..1766)
                     /locus_tag="VHH53_06835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX2289898.1"
                     /translation="MPATATTVPDVGALLATAVQALGGSERPGQVLMADAVDRAIRTG
                     EHLAVQAGTGTGKSLAYLIPAIRHAVTTDSTVIVSTATIALQRQLVDRDLPRLAGALE
                     PLLGSVPTFAILKGRRNYLCLHRLHTGPADEGEDLFEAASAASPTSMLGRQIARLHEW
                     SSQTDTGDRDELVPGVTDRAWRQVSVSARECLGMCRCPVGMDCFAEQARAQAGRAEVV
                     VTNHALLAIDAMGEHQVLPEHAVVVVDEAHDLVDRVTSAATEELSAAMVEAASRRCGR
                     LIDQQIADELAATGDGLALLLGDAPVQRWDTLAEMAVGALTAVRDAAHRCITALGSSE
                     RREEPNAATQRRLAQAALEQVHSAAARVLGAFPDAASANPEANRRDVVWLAEETVRGR
                     VLRVAPLSVAGLLRQQLFAERTVILTSATLTLGGTFDALAAQWGLPPQRFSAALAPAT
                     VTATATERPLPADGDEGVAWTGLDAGSPFVHHRAGILYTAARLPAPGRDGLAPAYLDE
                     LHALVSAAGGRTLGLFSSMRAAKQAAEVLRSRLDLPILCQGDDATGLLVAQFAKDEPT
                     CLFGTLSLWQGVDVPGRSLSLVV"
     gene            complement(1874..>2272)
                     /locus_tag="VHH53_06840"
     CDS             complement(1874..>2272)
                     /locus_tag="VHH53_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX2289899.1"
                     /translation="DPVDSYGVGTSVVTGSGVPTAGMIYKLVEVDGRPVAKRSASKTS
                     RGGAKSALRRYKPTGTAVEELVFPRGRQPEREKHDRVLPVPLLREGQPVDPLPTLQQA
                     RDRVHIALASVPWEGLRLSPGDPAIPTVFC"
BASE COUNT          342 a          797 c          806 g          327 t
ORIGIN      
        1 acgaccaaag acagcgaccg gcccggcacg tcgacgccct gccacagcga cagcgtgccg
       61 aacaggcagg tcggctcgtc cttggcgaac tgggccacca gcaggccggt cgcgtcgtcg
      121 ccctggcaca ggatcggcag gtccagccgg gatcgcagca cctcagcggc ctgcttggcg
      181 gcccgcatcg aggagaacaa gcccagcgtg cgcccacccg ctgcgctaac aagtgcgtga
      241 agttcgtcga ggtaggccgg agctagcccg tcgcgtccag gcgccggcag acgcgcggcg
      301 gtgtagagga tcccggcgcg gtggtgcacg aacggggagc ccgcgtccag gccggtccag
      361 gccacgccct cgtcgccgtc agcgggtagc ggccgctcgg tagccgttgc ggtgaccgtt
      421 gcgggggcga gtgctgcaga aaaccgctgc ggaggcaggc cccattgcgc ggcgagcgcg
      481 tcgaaagtcc cacccagggt gagggtggcc gaggtaagga tcaccgtgcg ctcggcgaac
      541 agctgctgcc gcagcagccc ggcaaccgac agcggggcaa ccctgagcac ccgcccgcgc
      601 acggtttcct cggccagcca gaccacgtcg cgccggtttg cttccgggtt agcagacgcg
      661 gcgtccggga aggcgccgag cacccgggcc gccgccgagt gcacctgctc cagcgcggcc
      721 tgcgccagcc ggcgctgggt ggcggcgtta ggctcctcgc ggcgttcgct gctgcccagc
      781 gcggtgatgc accggtgagc ggcgtcgcgc accgcggtca gcgccccgac cgccatctca
      841 gcgagggtgt cccagcgctg caccggcgcg tcgccgagca gcagcgcgag cccatcaccg
      901 gtcgcggcca gctcgtcggc aatctgctgg tcgatcagcc gcccgcagcg gcgcgacgcg
      961 gcctccacca tggcggcact gagctcctcg gtggccgcgc tggtgacccg gtcgaccagg
     1021 tcgtgtgcct cgtcgaccac caccacagcg tgctcgggga gcacctgatg ttcgcccatc
     1081 gcgtcgatcg cgagcagcgc atggttggtg accaccacct cggcacgccc ggcctgagcc
     1141 ctggcctgtt cagcgaagca gtccattccg actgggcagc ggcacatgcc caggcattcc
     1201 cgggcgctca ccgacacctg acgccaggcg cggtcggtca ctccgggcac caactcgtcg
     1261 cggtcaccgg tatccgtctg cgaggaccat tcgtgcagcc gggcgatctg gcggcccagc
     1321 atcgaggtgg gggacgctgc tgaggcggct tcgaacaggt cctcgccctc gtcggcggga
     1381 ccggtatgca gccggtgcag gcacaggtaa ttgcgccgtc ccttgaggat tgcgaaggtc
     1441 gggacgctgc ccagcagcgg ctccagcgcg ccggccagcc gaggcaggtc tcggtccacg
     1501 agttggcgct gcagcgcgat ggtcgcggtg gacacgatca cggtgctatc tgtggtgacg
     1561 gcgtgccgga tcgccggaat caggtaggcc agcgacttgc cggtaccggt gccggcctgc
     1621 acagcgaggt gctctccggt gcggatcgcc cggtcgaccg cgtcagccat caacacctgg
     1681 ccaggccgct cacttcctcc cagggcctgc actgcggtgg cgagcaatgc ccccacgtcc
     1741 ggcacggtgg tggcagtggc gggcacggcg ggccacgcta cagcttctag ctcaccgata
     1801 tggaaccatg tcggtgttgt agcgggctcg gttaccccaa catggcacca tatcggcgat
     1861 aagggggtaa gggtcagcag aagaccgtgg ggatggctgg gtcgccgggg gacaggcgca
     1921 gcccctccca ggggacgctg gctaaagcta tgtgaactcg gtcacgagcc tgctgcaggg
     1981 tgggcagtgg gtcgaccggc tggccttcgc gcagcaacgg caccggaagc acccggtcgt
     2041 gcttctctcg ctcaggctgc cggccccgcg ggaacaccag ctcctcgacc gcggtgccgg
     2101 tgggcttgta ccggcgcagg gcgctcttgg ccccgccccg ggaggttttg gaggcactgc
     2161 gtttggctac tggccggccg tcgacctcga ccagcttgta gatcattccg gcggtcggca
     2221 cccccgagcc ggtcaccacc gaggttccta cgccgtagga gtccaccgga tc
//
LOCUS       DATADN010000528         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_374222, whole genome shotgun sequence.
ACCESSION   DATADN010000528 DATADN010000000
VERSION     DATADN010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374222"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VHH53_06845"
     CDS             <1..823
                     /locus_tag="VHH53_06845"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2289900.1"
                     /translation="AAHHPVGDQQRLRALEMATGRILLVRTKPVQVTESGQAVLRLAR
                     QVELLTADVARELGGDPSPGRVPTLPVAVNADSMATWVLPALTPLAGDVRFDLYREDQ
                     AHTSALLRAGTVMAAVTADAEPVPGCTSTRLGGMRYRPMATPGFARRWFPAGPTAEAL
                     TRAPIVVFDRKDDLQHRYLHAQAGPDVAPPVHYVPAAADYVAAITLGLGWGMVPDLQA
                     RGVTAELVVLEPAGAMDVVLYWQQWRLCSPTLDRVREAVLAAAGRELDQPGGSRR"
     gene            1206..1892
                     /locus_tag="VHH53_06850"
     CDS             1206..1892
                     /locus_tag="VHH53_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX2289901.1"
                     /translation="MSGNQQAKLVALLGVATTAVGIGGTLVPDASAAATSSSSPSFQY
                     EIREVTAADLPYTYRSGCPVPPEDLRLLIMSHRTFDGQIAQGEMVVHEDWASPVVSVF
                     HELYDQDFPIARMQLVDDYQGSDDLSMEANNTSAFNCREVTGRPGVWSQHSYGGAIDI
                     NPVQNPYISSGGTVLPPAGADYVDRSKTAPGMIHAGDCTVQAFTDIGWYWGGAWTNPK
                     DYQHFSHNNR"
     gene            2146..2676
                     /locus_tag="VHH53_06855"
     CDS             2146..2676
                     /locus_tag="VHH53_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289902.1"
                     /translation="MARSDLAGTAGYSYCPSHSRWHRGLRLHLICTPADLPITWALAY
                     AEIDERQMLMCLLDHDADLLADRPGLLLLADKGYVPAELDDYLHARGADLLRPSYRNR
                     TPRPGQALLAPIRQLIESVHEALRGQLALELHGGRSLTGVTARIAQRLLALTPAIWRN
                     RATGQPITRSLIAYDH"
BASE COUNT          420 a         1064 c          902 g          387 t
ORIGIN      
        1 cgctgcacat caccccgtcg gcgatcagca gcggctgcgt gcgctggaga tggcgaccgg
       61 ccggatcctg ctcgtgcgga ccaagccggt gcaggtcacc gagtccgggc aggcggtgct
      121 gcgcctggcc cggcaggtcg aactcctcac cgccgacgtc gcccgcgagc tcggtgggga
      181 cccctcgccc ggtcgggtgc ccacactgcc ggtcgcggtc aacgccgact cgatggccac
      241 ctgggtgctg cccgcgctga cgccgctggc cggcgacgtc cgcttcgacc tgtaccgcga
      301 ggaccaggcg cacaccagcg ccctgctgcg cgccggcacg gtcatggcgg cggtgaccgc
      361 ggacgccgag ccggtgcccg gctgcacctc cacccggctg ggtggcatgc gctaccggcc
      421 gatggccacc cccggtttcg cgcggcggtg gttccccgcc ggccccaccg ccgaggcgct
      481 cacccgggcc ccgatcgtcg tcttcgaccg caaggacgac ctgcagcacc gctacctgca
      541 cgcgcaggcc ggtcccgacg tcgccccacc ggtgcactac gtgcccgccg cggcggacta
      601 cgtcgccgcc atcaccctgg gcctgggctg gggcatggtg cccgacctcc aggcgcgcgg
      661 agtcacggcg gagctcgtcg tcctcgaacc agccggcgcg atggacgtcg tcctgtactg
      721 gcagcagtgg cggctgtgct cccccacact cgaccgcgtc cgcgaggccg tgctcgcggc
      781 cgccggccgc gaactggacc agcccggcgg gtccaggcgc tgatcgaccc agcagcacgc
      841 tgccgccaac ccctcatgcg caggttgcga gcgcaccggg atcgtggccg tcgagcgcgc
      901 cgacaccctg gccgacgggt ggcggctccg gctcgactgg cagcgggccg tcgcccccga
      961 caaccagacc gagagcaaga cgctcgaggc cgaccgcggc tacatccgtg tgacagcccg
     1021 tcgccgaggc gaggccgagc tgggagacta ctgccggccc gacaccctgt gcttgtcaca
     1081 cgacgagtgc acgcgcacgc cgctgctccg tggggagagc ccgtcccccg atgtatcggg
     1141 agttccagag cgagcgtctg acgacgcacg gtgcatgacg gcctgttcgc aaggaagggg
     1201 ttcccgtgtc cggcaatcag caggccaagc tcgtggccct tctcggtgtg gctaccacgg
     1261 ccgtcggcat cggcgggaca ctggtgcctg acgcgtccgc agccgccacg agctcgtcct
     1321 cgccgtcctt ccagtacgag atcagggagg tcaccgccgc agatctgccc tacacctacc
     1381 gttccggctg tccggtgccg ccggaagacc tgcggttgct gatcatgagc catcgcacgt
     1441 tcgacgggca gatcgcgcag ggcgagatgg tcgtacacga ggactgggcc agtccggtgg
     1501 tctcggtgtt ccacgagctg tacgaccagg acttcccgat cgcacgcatg cagttggtcg
     1561 acgactacca gggcagtgac gatctgtcga tggaggcgaa caacacctct gccttcaact
     1621 gccgggaggt gaccgggcgc ccaggggtgt ggtcgcaaca ctcctatggt ggggccatcg
     1681 acatcaaccc cgtgcagaac ccctacatct cctccggtgg cacggtcctc ccccctgccg
     1741 gcgccgacta cgtcgaccgg tcgaagacag cacccgggat gatccacgcc ggcgactgca
     1801 cggtgcaggc cttcaccgac atcggctggt actggggcgg cgcctggacg aaccccaagg
     1861 actaccagca cttctcccac aacaacaggt gagacgcctc ccgtcggaac cggacatcgt
     1921 gcctgacccc gcctgagcgc tcgcacacca tcccgcatcg aggtcgcagg tggacgcggg
     1981 cagacctggc agtggcagct gaccagccag catcgacggc gtcggttgtg gcgttgcgat
     2041 ggtcagcgag cagcgtcggc ctgtacgccg ggaactgtcg atgggtgatt ggccaacgtc
     2101 ggcagttgcg gatcacgcgt ctggcgcgcc cgctcccgcc cgaccgtggc ccggtcggac
     2161 ctggccggca ccgccggcta cagctactgc cccagccact cccgctggca tcggggcctg
     2221 cggctgcacc tgatctgcac cccggccgac ctgccgatca cctgggcgct ggcctacgcc
     2281 gagatcgatg aacgccagat gctcatgtgc ctgctcgacc acgacgccga cctgctcgcc
     2341 gaccggccgg gcctgctgct gctcgccgac aagggctacg tccccgccga gctcgacgac
     2401 tacctgcacg cccggggcgc ggacctgctg cgcccctcct accgcaaccg cacgccccgc
     2461 cccggccagg cgctgctggc gccgatccgc cagctgatcg agtccgtcca cgaggccctc
     2521 agaggccagc tcgccctgga actccacggc ggacgcagcc tcaccggtgt gaccgcccgc
     2581 atcgcccaac ggctcctggc gctcaccccc gccatctggc gcaaccgcgc caccggccag
     2641 ccgatcaccc gatccctgat cgcctacgac cactgaccgg agatcggact taactcatct
     2701 agccgcggcg acccctctgg cctacgccgg acagctgccg catccagagg acgcggtgca
     2761 ggcgaagacc acg
//
LOCUS       DATADN010000529         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1427380, whole genome shotgun sequence.
ACCESSION   DATADN010000529 DATADN010000000
VERSION     DATADN010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427380"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VHH53_06860"
     CDS             <1..884
                     /locus_tag="VHH53_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX2289903.1"
                     /translation="PMWIKPVKSASFELAFGVSNQQEFHDALKQIRQRISRMGAPFEA
                     VLDYLELPPEIANVGGQVCLAEEEVSGRQLTFEGYRHRGETHTLGVVDSIRYEHSPSF
                     LRYQYPASIPDRVAERMADISDRVVRQVELEHATFNIEFFWDDNTDALYVLEVNPRHS
                     QSHAELFAQVDGAPNHLAMLRLALGRDPEMPRRQGPYQVATKWFLRHWNDGVVRRQPT
                     ELEIEQVQREIPGVTVDVTAHEGDRLSELPAQDSYSYILANIYVGAQDEAELTEKYRR
                     CVRALPFDIDDQEVPSA"
     gene            884..2908
                     /locus_tag="VHH53_06865"
     CDS             884..2908
                     /locus_tag="VHH53_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX2289904.1"
                     /translation="MREVTSLPYAVRAGEQVRIKMFDGVRLMARIWRPISSDDEPVPA
                     VLEFIPYRHRDLTAWRDSIQHPYLAGHGYAAVRVDLRGSGDSEGLLRDEYLEQELTDA
                     EEVLAWMATQPWCSGRTGMMGISWGGFNALQLAARRPPGLGAIVTACSTDDRYADDVH
                     YMGGCLLSDNLSWASSMFAYNSCPPDPAVVGERWRDMWHERLTGSGLWLETWLRHQRR
                     DSYWQHGSVCENFADIAVPVYAVSGWADGYSNAVFRLLAELDVPRKGLIGPWSHRYPH
                     LGQPGPAIGFLQEVVCWWDRWLKDIDNGIMDEPMLRIWMQDTVPPSTAYEQRPGRWVG
                     EPAWPSPHVYESIHPLARHRIAGLDERLDAEELTIQSPLSVGQFAGKWCSYSAPPDLP
                     YDQREEDGGSLVFDSDPLTERCEILGAPVLEFSFASTEPVAMVAVRLSDVAPDGRATR
                     ISYGLLNLTHYKGHNAPELLVPGQRYQAAVQLNGMAQALPPGHRLRLAISTSYWPLAW
                     PPPRLVCLSIFTHHTCLRLPVRPIREPDDVPVRPFGDPEGTPPIPVTQLQPGEERWTV
                     SRDLVNYRSTLEVIKDRGTQRFDDIDLDVARRTIERYSSSDGDPCSVCGETQSTVSFG
                     RGEWQVQAVTRTVLTSTPTDFQVHAQLDAYEGAQRVHSSNWQRVIPRDHV"
BASE COUNT          518 a          973 c          959 g          521 t
ORIGIN      
        1 acccgatgtg gatcaaaccg gtgaagtcgg catcctttga gctcgccttc ggggtgtcca
       61 accagcagga gttccacgat gcgctgaaac agatccgcca gcggatcagc cggatggggg
      121 ccccgttcga agccgtcctc gactatctcg aacttccgcc agagatcgcc aacgtcgggg
      181 ggcaggtgtg cctggccgag gaggaggtca gtggcaggca gctgaccttc gagggctacc
      241 gccaccgcgg cgagacccac acgctcgggg tggtggactc gatccggtac gaacacagcc
      301 ccagcttcct gcggtaccag taccccgcat ctatcccgga ccgggtcgcc gaacgaatgg
      361 ccgacatctc cgatcgtgtc gtgcggcagg tcgagctgga gcacgcaacg ttcaatatcg
      421 agttcttctg ggacgacaac accgatgccc tgtatgtgct ggaggtcaac ccgcggcatt
      481 cccagtcgca cgccgagctg ttcgcccagg tcgacggcgc gcccaaccac ctggcgatgc
      541 tccggctggc gctgggccgc gacccggaga tgccacgccg ccagggtccc taccaggtcg
      601 cgaccaagtg gtttctgcgg cattggaatg acggtgtagt ccgccgccaa cccaccgagc
      661 tggagatcga acaggtacag cgggagatcc ccggtgtcac cgtcgacgtg accgcccatg
      721 agggtgaccg gctctcagag ctgcccgccc aggacagcta cagctatatc ctcgcgaaca
      781 tctacgtcgg tgcccaggac gaggcagagc tgaccgagaa gtaccggcgg tgcgtccggg
      841 ccctgccctt cgacatcgat gaccaggagg tgcccagcgc ctgatgcgcg aggttacatc
      901 cttgccttat gcagtcaggg caggtgagca ggtccggatc aagatgtttg atggcgtgcg
      961 gctgatggca cggatctggc gacccatctc ctccgacgac gagccggtac cggccgtcct
     1021 ggagttcatc ccataccggc atcgcgacct caccgcctgg cgggactcca tccaacaccc
     1081 ttacctggcc gggcacggct acgccgcggt acgggtcgac cttcgcggca gtggcgactc
     1141 ggagggcctg ctccgtgacg agtacctcga acaggagctc accgatgccg aggaggtgct
     1201 tgcctggatg gccacccagc cgtggtgcag cgggcgaacc ggcatgatgg gcatctcctg
     1261 gggcgggttc aatgcactgc agttggcggc gcgccggccg cctggcctcg gggcgatcgt
     1321 cacggcgtgc tcgactgacg accgctacgc cgatgacgtg cattacatgg gtggctgctt
     1381 gctttcggac aatctttcgt gggcttcgtc gatgttcgct tacaactcct gcccgccgga
     1441 tcctgctgtg gtcggcgagc gctggcggga catgtggcat gagcgcctca ccggcagcgg
     1501 gctgtggctg gagacgtggt tgcgccacca gcggcgggac agttactggc agcacggctc
     1561 ggtgtgtgag aacttcgccg atatcgcggt cccggtttac gccgtttccg gatgggcgga
     1621 cgggtactcc aatgcggttt tccggctgct cgccgaactt gacgtaccgc gtaaaggttt
     1681 gatcggcccg tggtcgcaca ggtatccaca cctcggccag ccaggaccag ccatcggttt
     1741 tctgcaggag gtcgtgtgtt ggtgggaccg ctggctcaag gacattgata acggcatcat
     1801 ggatgagccg atgctgcgga tctggatgca ggatacggtg cccccgtcta ccgcctacga
     1861 gcagcgaccc ggccgctggg tgggtgaacc agcctggccg tcgccgcacg tgtacgagtc
     1921 catacacccg ctggcgcggc accggatcgc tggtctggac gagcgcctcg acgccgagga
     1981 gctgaccatc cagtcaccgc tgtcggttgg tcagttcgcc ggcaagtggt gctcctacag
     2041 tgcgcctccc gacctaccct atgaccagcg ggaggaagac ggtggatcac tggtgttcga
     2101 ttccgatcca ctcaccgagc gctgtgaaat cctcggtgca ccggtgctgg aattctcctt
     2161 tgcctccacc gagccggtgg cgatggttgc ggtgcggcta tccgacgttg cacccgacgg
     2221 gcgcgctacc agaatcagct acggcctgct caacctcacc cattacaagg ggcacaacgc
     2281 gcccgaactg ctggtgccgg gccagcggta tcaagccgcc gtgcagctca acggcatggc
     2341 gcaggccctg ccgcccggtc atcggctgag gctggcgatt tccacctcat actggccgct
     2401 ggcctggcca ccgccgcgac tagtgtgcct gtcgatcttc acccatcaca catgtctgcg
     2461 gctgccggtg cggcccatcc gcgagcctga tgacgtgcca gtgcggccgt tcggcgaccc
     2521 ggaagggacc ccgcctatcc cggtgaccca gctacaaccg ggcgaggaac gctggaccgt
     2581 ctcccgagac ctggtcaatt accgctcgac acttgaggtg atcaaggacc gtggcacaca
     2641 gcgcttcgat gacatcgacc tggacgtggc cagacgaacc atcgagcgct acagctccag
     2701 cgacggcgac ccgtgctcgg tctgcggcga gacacaatca acggtgagct tcggccgcgg
     2761 ggaatggcag gtgcaggccg tcacccgcac cgtgctgacg tctacgccca ccgactttca
     2821 ggtgcatgct caactcgacg catacgaggg tgcccagcga gtgcactcat ccaactggca
     2881 gcgggtgatt ccccgcgatc acgtctgacg tagcttgcgc ctgaagcagc gccatgacag
     2941 aggaagaccc cgtgcggctg ccgggtcggg g
//
LOCUS       DATADN010000530         4462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_668033, whole genome shotgun sequence.
ACCESSION   DATADN010000530 DATADN010000000
VERSION     DATADN010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668033"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VHH53_06870"
     CDS             complement(<1..492)
                     /locus_tag="VHH53_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2289905.1"
                     /translation="MPAPGTPLDQLGFLTIGTFDPADPGPGHEATLAMVELGEQLGFD
                     SAWLRHRHLQHGISSPVAVLAAATQRTRRIRLGTAVTPLSWENPLRLAEDLVTVDVLS
                     GGRLNPGVSVVPPMRWDDVRASLYPDTADAEDFGYGRVSRLLRLVAGERAGTSAGREG
                     VVEE"
     gene            1126..2691
                     /locus_tag="VHH53_06875"
     CDS             1126..2691
                     /locus_tag="VHH53_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289906.1"
                     /translation="MREHKGPFGTPDGSRADLEDILRDFVALDGRPGFGGVATRADDA
                     MVRVIVGRIGAGKTVYMRRLFSYQEASKSVYADPPQQDLPQTDLVIQACQWFPSELLT
                     EKWMQLWHRAILRSLTTHLLTARQLRGTVEAAEAAAIRGEYGHLIGKFRQPRSIYSEL
                     RDIIFSHNAAIHLRRYLENPDWDDLESVLADLLRESMPVFFYLDAVDERINSAPMYWL
                     RCQEGLFMQVMQLLRDSRFGSRLHVVVCVRELVLASIYRSEHAPRYHNEPHIRVLDWS
                     REAIEFLLRQKIERLTDEFLMAPSLPSGSLSWLGRTWVHNEARGVDEDVLEYIIRHTR
                     LIPRDIVSLGNDLCQVVAQQKSLGRTEVPGEVLRRVVSLAAKRFGDSQLAQCASQIAA
                     DAIPRHAVKKGFADFYLSDQEYARSLRQELKEIIRAVGVDRFDAGDLRQMEELAKQGF
                     DGATSIASILWQNGLLGYVENGRYSFYSVSDMNELELPRHAHEYVFHPCLMDAVPGLR
                     PSGREVVYPYYRE"
     gene            2701..3456
                     /locus_tag="VHH53_06880"
     CDS             2701..3456
                     /locus_tag="VHH53_06880"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEX2289907.1"
                     /translation="MAKVARLALHSWEELKRDLVPLLFDREPFRSGEYLFRGCGDAEW
                     SLISSFDRQFGHLSSDKRMAIWKALLREFREQGAEHGVAREILADDKALLAFAQHHGL
                     PTRLLDWSLSPYVAAFFAFRQALKSPGATGQVALWVLDTRSSAWSAEMGVELVAPFAS
                     GNLRLRNQSGRFTYARTPHLTLEEYVSYVPTDESLLTQVTLPSIEAIRAMPDLDAMGT
                     HAINLFPDWEGLAEGVAIRLELEALASSEAPVS"
     gene            complement(3604..3831)
                     /locus_tag="VHH53_06885"
     CDS             complement(3604..3831)
                     /locus_tag="VHH53_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289908.1"
                     /translation="MSSASPSTSSASYAASIAWRACSRRSSAAFSARISTPTGNRALL
                     DGRSSSRTTSAASIGVRREPADVMTEHTYGV"
     gene            3910..>4462
                     /locus_tag="VHH53_06890"
     CDS             3910..>4462
                     /locus_tag="VHH53_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX2289909.1"
                     /translation="MSQFFTSELAMVLNCGRRTAANLALRAWTYREQLPATWAALAAG
                     TLDEPRAKVLADVLGLTSPALARAVEAQLLPEATGLSTGRLRARALALLLELDAAAGD
                     ARRTEARRQADVRIYPSPLEGMAILAADLPTPVSAECYSVVDRLAAMLEADGDPRPIG
                     QLRAAVLADLIRRPWDTGLPAVTA"
BASE COUNT          720 a         1467 c         1500 g          775 t
ORIGIN      
        1 ctcctcgacc acgccctccc ggccggcgga ggtaccggcc cgctcgcccg cgaccagccg
       61 cagcagccgg gacacccgcc cgtagccgaa gtcctcggcg tcggcggtgt ccgggtagag
      121 cgacgctcgc acgtcgtccc agcgcatcgg cggtacgacg ctcacgcccg ggttcagccg
      181 gccgccggag aggacgtcga ccgtgacgag gtcctcggcc agccgcagcg ggttctccca
      241 cgacagcggc gtgacggcgg tgccgaggcg gatccggcgg gtgcgctggg tggcggccgc
      301 gaggacggcg accggcgagg agatcccgtg ctggaggtgc cggtggcgca gccacgcgct
      361 gtcgaagccc agctgctccc cgagctcgac catcgccagc gtcgcctcgt gcccggggcc
      421 ggggtcggcc gggtcgaagg tgccgatcgt caggaagccg agctggtcca gcggggtccc
      481 gggtgcgggc atggggcggc tcctcgatcg gtgcggcggg gtccgtgcca tcgtcggtca
      541 gtggccgacg acgtgctcct gcgccgtccc cgcggagggg gcgtggtggg accccagtag
      601 cgccgcaggt ccaccgcggg gagggagtca gggtcgcgtg ggggctgtcg tgcgggtcga
      661 gcgcgccgcc actgcaccga tgtctctgag cggcccactg tgggacagac ccatgtcgca
      721 cccgacccga ctccgtgctc ccgtgctctc ggatgaacgg cagcctctgc gaaccggtcg
      781 acgcgtccct gggcctgctc ggtcccgcaa tgtgacgacc cggcactgcc tcacccggca
      841 ttcggctttt catgggccgc tgagcgccaa gccccggcga cacgcaccag cgaagccctg
      901 gaccggcgcc agttcctcac tcagcgtatg taacccttca cttgcgcgac gacacaccgc
      961 agggacaggg gcaccgactg tttcgactca ctcggcccta ggcccggccc ccagagggaa
     1021 ctaccgtgcg ccaaaaccct gcggaaccga ctcagtacgg actgctggtg aacttcgtcc
     1081 gcagcgatgc agaggcgctc aatgactcgg ccgccgactt cgaacgtgag ggagcacaag
     1141 ggtcccttcg gcacgccgga cggttcacgt gcggacctcg aggacatcct gcgcgatttt
     1201 gtcgccttgg acggaaggcc tggctttggc ggagtggcaa cgcgcgccga cgatgccatg
     1261 gttcgggtca tcgttggtcg gataggggct ggcaagaccg tatacatgcg gcgtctattc
     1321 agctaccaag aggccagcaa gtcagtctac gccgatccgc cccaacagga cttgccccag
     1381 accgacctgg tgatacaggc atgtcagtgg ttcccgagcg agctcctcac cgagaagtgg
     1441 atgcaactct ggcaccgtgc gattctgcgc agcctcacaa cgcatctgct cacagctcgg
     1501 caattgcgtg gcacggtaga agcggcagag gcggccgcga tccggggaga gtacgggcac
     1561 ctcattggta agttccggca acctcggtcc atctatagcg agcttcgtga cataattttc
     1621 agtcacaatg cggctattca cctgcggcga taccttgaga atcccgactg ggacgatctg
     1681 gagtcggtcc ttgcggacct gctgcgagag agcatgccgg tcttcttcta tctggatgcc
     1741 gtggacgaaa ggatcaacag tgcacccatg tactggctcc gatgccaaga aggcttgttt
     1801 atgcaagtca tgcaattgct ccgggactcg aggttcggct cacggttgca cgtcgtggtg
     1861 tgcgtgcgcg agctcgtgct tgcctccatc tatcgaagcg agcatgcccc gcgctatcac
     1921 aacgaaccgc acatccgcgt gttggactgg tctcgggagg cgatcgagtt tctgttacga
     1981 cagaagatcg agcgactgac tgacgaattt ctgatggccc catcactacc cagcggatcg
     2041 ttgtcatggc tgggacgcac ctgggtgcac aacgaagctc gcggcgtgga tgaagatgtt
     2101 ctggagtaca taatcaggca tacacgactt attccacgcg acatagtctc cctcggcaac
     2161 gatctttgcc aggtggtcgc gcagcaaaaa tcgcttgggc gtaccgaagt tcctggagag
     2221 gtgctgcgcc gcgtggtgtc cttggccgcg aagcgcttcg gcgactctca gttggcgcag
     2281 tgtgccagcc agatcgcggc cgatgcgata ccccgccatg cggtgaagaa gggttttgcc
     2341 gacttctacc tgtccgacca ggagtacgcc aggtccctgc ggcaggagct caaggagatc
     2401 atacgagccg taggtgtgga tcgattcgac gccggagacc tgcgccaaat ggaggaactc
     2461 gccaaacagg gcttcgacgg ggcgacgagc attgcgtcga tcctttggca gaacggcttg
     2521 ctcggatacg tcgagaacgg ccggtacagc ttctattccg tctcggacat gaatgaactc
     2581 gagttaccgc gacatgctca cgagtacgtc tttcacccct gcctcatgga cgccgtgcct
     2641 ggattgcgtc cgtcggggcg ggaggtcgtc tacccttact accgggaata ggagggcagt
     2701 gtggccaagg ttgctcgcct cgcgcttcac tcctgggaag aattgaagcg cgacctggtg
     2761 cctctgctct ttgatcgaga accattccga tcaggcgagt acctgttccg tgggtgcgga
     2821 gacgccgagt ggtcgttgat atcatccttc gatcgacagt tcggtcatct gtcgagcgac
     2881 aagcgcatgg cgatctggaa ggctctgcta cgggagttca gagaacaagg cgctgagcat
     2941 ggcgtggccc gcgagatcct cgcggacgac aaagcgctgc ttgcgttcgc tcagcatcac
     3001 ggattgccaa cgcggttgct ggactggtcg ttgagcccct acgtggccgc cttctttgcc
     3061 ttccgtcagg cgttgaaaag ccccggcgca accggtcagg tggcgctatg ggtccttgac
     3121 acccggtcca gcgcttggtc agcggagatg ggggtcgagc tggtcgcccc tttcgcgtca
     3181 ggcaatctcc gactgcgtaa ccagtctggt cgcttcacgt atgctcgcac ccctcacttg
     3241 accttggagg agtacgtcag ctacgtgccg acggacgaaa gccttttgac gcaggtcacc
     3301 ttgcccagta tcgaagccat ccgcgctatg cccgatctcg acgccatggg cacacatgcc
     3361 atcaatctgt tcccggattg ggagggcctt gctgagggag tggccatccg actcgaactg
     3421 gaggcgctgg cgagtagcga agctccggtc agctgaccgt cctggctgtg ctctggcgtt
     3481 cccgcgggtg catggggcag ggccgctgtc gcctgtccca gaggccgcca tcagggacac
     3541 gggtggcggc gcagaggatc tgcaggacag gcactcgtcg tcatgtccga tcgcccgaaa
     3601 ccgtcagacc ccgtacgtat gttcggtcat gacgtcggcc ggctccctgc gcacgccgat
     3661 cgaggcggcg ctggtggtcc ggctgctcga gcggccgtcg agcagcgccc ggttgccggt
     3721 gggtgtgctg atccgggcgg agaaggccgc cgagctgcgc cggctgcagg cccgccaggc
     3781 gatggacgcc gcgtatgagg ccgagctggt gctcggactg gccgacgaca cccccgacac
     3841 cctggacccg ccgccggacg gcccggtgcc cggcgcgggt cgtgggcgcc ggagcccgag
     3901 ctgcccgggg tgtcacagtt cttcacgtct gagctggcga tggtgctcaa ctgcggccgc
     3961 cgcaccgcgg cgaacctggc cctgcgggcg tggacctacc gcgagcagct gccggccacc
     4021 tgggcggcgc tggccgccgg caccctcgac gagccccgcg cgaaggtgct ggccgacgtc
     4081 ctcggcctga cctctcccgc gctcgcccgg gccgtcgagg cccagctgct gcccgaggcg
     4141 accggtctgt cgaccggccg gctgcgggcc cgggcgctgg cgctgctgct ggagctggac
     4201 gccgccgccg gggatgcccg ccgcacggag gcccgccggc aggccgacgt gcgcatctac
     4261 ccctcgccgc tggagggcat ggccatcctg gccgcggacc tgccgactcc ggtgtcggcc
     4321 gagtgctact cggtggtcga ccggctggcc gcgatgctcg aggccgacgg cgatccccgg
     4381 ccgatcgggc agctgcgcgc cgcggtgctc gccgacctca tccgccggcc gtgggacacc
     4441 ggcctacctg cggtcaccgc cc
//
LOCUS       DATADN010000531         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_521007, whole genome shotgun sequence.
ACCESSION   DATADN010000531 DATADN010000000
VERSION     DATADN010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521007"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..312
                     /locus_tag="VHH53_06895"
     CDS             67..312
                     /locus_tag="VHH53_06895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2289910.1"
                     /translation="MIGNGVVGVDRAVADDGWAGVSGMATQPHARGRGAARSVLAALA
                     DWVGAHEADRTYLRLALRLYERTGFDEVGGYHYRTPG"
     gene            403..1626
                     /locus_tag="VHH53_06900"
     CDS             403..1626
                     /locus_tag="VHH53_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2289911.1"
                     /translation="MHFTNLLIIMVVALIAPLVVSALPALRVPSPVLEIVAGIVIGPS
                     VLGWVGIDAPVQVLALVGLAFLLFLAGLEIDVHQLRGRPLTLALTGFVVSFAIAVGAA
                     LLLTAGGFASTPLLIAIILCSTSLGLVVPVLKDSGQVAGATGQLIIAAASIADFGAVI
                     LLSLFFSEESTGLAARLLLLAGLALVVVLLAVALMRAGRTARADRVFQMLMDTTAQIR
                     IRFAMVLLIAFVALAQGLGFEAILGAFLAGAVLRLIDADAATVHPHTELKLDAIGYGF
                     LIPVFFITSGVMFDAKALFASPSTLLQVPLFLAALLVVRALPALLYRAELGMRRTIAA
                     GFLQATSLPFIVAAVQVGQRLGALPPASGAALIAAGLASVLFFPAIALAVMRSAPSED
                     RVLFPSPLGRKSPGR"
     gene            complement(1801..1935)
                     /locus_tag="VHH53_06905"
     CDS             complement(1801..1935)
                     /locus_tag="VHH53_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289912.1"
                     /translation="MREPFRGSVLEATLERITVAGRGVLGSGEHTLSEPVPCLPGGWQ
                     "
BASE COUNT          277 a          707 c          695 g          417 t
ORIGIN      
        1 agcgactccc gtaccgagtg aaacatgctc ggtcgtgtgc agcggccgag tgcctacgct
       61 agcgccatga ttgggaacgg tgtcgtcggg gtggaccgcg cggttgccga cgacggctgg
      121 gcgggcgtgt ctggcatggc cacgcagcct cacgcgcggg gcaggggcgc cgctcgtagt
      181 gtgctcgctg cgctggcaga ttgggtcggt gcacacgaag ccgaccgtac gtatctccgt
      241 ctggcactcc ggctgtacga gcggacgggt ttcgacgagg tgggcggtta ccactatcgc
      301 accccaggat gaacctcgtg atctgcccat ggcgaaccac cgtctacgac ctcggagtgc
      361 tcgacgccct cgcggacctg cccgcctaag tgaggtggcc cgatgcattt caccaacctg
      421 ctgatcatca tggtggtggc gctcatcgcg ccgctggtgg tcagcgcgct gcctgcgctg
      481 cgagtgccct cacctgtact ggagatcgtg gccgggatcg tgatcggtcc ctcggtgctc
      541 ggctgggtag gcatcgacgc tccggtccag gtgctcgccc tggtcgggtt ggcgttcctg
      601 ctatttctgg ccggtctgga gatcgacgtg caccagttgc gtggccgccc gctgaccctg
      661 gcgctgaccg ggttcgtggt gtccttcgcg atcgcggtcg gggccgcgtt gctgctgacg
      721 gccggtgggt tcgcctcgac accactgctg atcgcgatca tcttgtgttc cacctcgctg
      781 ggactggtgg tgccggtgct caaggactcc ggccaggtcg ccggcgccac cgggcagctg
      841 atcatcgccg cggcgtccat cgccgacttc ggtgcggtga tcctgctgtc gctatttttc
      901 tccgaggaat ccaccggtct cgccgcccgg ctgctgctgt tggccggtct ggcgctggtc
      961 gtggtgctgt tggcggtcgc gctgatgcgg gccggccgca cagcccgcgc cgaccgggtc
     1021 ttccagatgc tgatggacac caccgcgcag atccggatcc ggttcgcgat ggtgctgctc
     1081 atcgcgttcg tggcgctggc tcaaggtctg gggttcgagg cgatcctcgg cgcgtttctg
     1141 gctggagccg tgctgcggct gattgacgcc gacgccgcga cggtacatcc gcacaccgag
     1201 ctgaaactgg atgcgattgg ttacggcttt ctcatcccgg tgttcttcat caccagcggg
     1261 gtgatgttcg acgcgaaggc gctgttcgcc tcaccctcga cgttgctgca ggtaccactg
     1321 tttctagcgg cgctgttggt ggtgcgggcc ctgccagcgt tgctctatcg agcagagttg
     1381 gggatgcggc gcaccatcgc ggcaggtttc ttgcaggcca cctcgctgcc gttcattgtc
     1441 gctgcggtac aggttgggca acggcttggc gcgttgccgc cagcttccgg tgcggcgctg
     1501 atcgcggccg gtctggcctc agtgttgttc ttccccgcga tcgccctggc ggtcatgcgc
     1561 agcgccccgt ccgaagaccg tgtgctgttc ccatcgccac tcggccgcaa gtcaccaggt
     1621 aggtaggcgg acggcactgc tgtcgccgcg gacgctgtcc atgcgatgcc gcgggccgac
     1681 ctagcacaac cggctcaccg cagcgggccg cctgaccgcc ctatgttctc gaccgagaat
     1741 aacgcagcgg cgtaacggga catcggcgcg gcggcactga ccggtagggc tcttccagac
     1801 tcactgccag ccacccggca ggcatggcac cggctcgctg agcgtgtgct cgccgctgcc
     1861 cagtacgccg cgaccggcga ccgtgattcg ctccaaagtc gcctccagca ccgacccgcg
     1921 aaacggctcc ctcacgtctg tccctcacca aatttctccg cgccacccga tgctcgcgcc
     1981 acatctatca cctctccggc tacaccagtt ccccggatgc ccacccctgg ggctgttcgc
     2041 ggtggatcgt cgggacaacc ggcgcctgca tggcgaacac tcgacgcacg agcagg
//
LOCUS       DATADN010000532         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1427706, whole genome shotgun sequence.
ACCESSION   DATADN010000532 DATADN010000000
VERSION     DATADN010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427706"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VHH53_06910"
     CDS             complement(<1..864)
                     /locus_tag="VHH53_06910"
                     /inference="COORDINATES: protein motif:HMM:NF044706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289913.1"
                     /translation="MPVVDLADVQGLILRGYTMPAARYIGLAVREPRAARAFLGSLIT
                     SDAAVSVTTAQPWETKPQYCVNLGITFPGLAALGVPPVSRDSFPVEYAQGAVERATRV
                     GDVGISSPQRWLPWLADSGLHLLVMVFAQSSDALDAATEQLEHAWSAGCVERGRHDGT
                     WLPGPLTHFGYRDGLSQPVIADVPLSGLPDHLPRAPVGEFLLGYPSQHVGFSYPVPAP
                     PELGINGSFGAFRLLEQDVDAFAESVAEAASANGVSKEFIKAKLCGRWPNGMPLVLSP
                     DTDTPDPPDPAR"
     gene            complement(889..1338)
                     /locus_tag="VHH53_06915"
     CDS             complement(889..1338)
                     /locus_tag="VHH53_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289914.1"
                     /translation="MTGPSPPVQNPLTMVMKAKSPDDYAALRKTVEHLQSLPPDKNPV
                     TIALNKIVTVHFARFTFLDNDQLAVITSYDGDFEVYFNEFINEIGDVFNMLLEHVEDG
                     PPLPVQTYRQEFLEYIRAHDFRCVGTFYSAYPDRTVLDILSAATASR"
BASE COUNT          299 a          483 c          507 g          308 t
ORIGIN      
        1 tcgggcggga tcgggggggt cgggtgtgtc ggtgtccggt gacaggacga gtggcatgcc
       61 attgggccag cgtccacaca gcttcgcctt gatgaactcc ttcgataccc cgttcgcaga
      121 cgccgcctcc gcgacggatt ccgcaaacgc gtcgacgtcc tgctcgagca gccggaaagc
      181 accgaagctg ccattgatac cgagctccgg cggcgccggc accggataac tgaaaccgac
      241 atgctggctg ggatatccga gcagaaattc accgaccggt gcccgtggta gatgatccgg
      301 cagcccggac agcgggacat ccgcaatgac gggctgagac aggccgtcgc gataaccgaa
      361 atgggtgagg gggcccggca gccacgtgcc gtcgtggcgt cctcgctcga cgcagccggc
      421 tgaccaggca tgttcgagtt gctccgtcgc ggcgtcgagg gcgtccgacg actgggcgaa
      481 gaccatgacc agcaagtgca gcccggaatc agccaaccac ggcagccacc gctgcgggct
      541 gctgataccg acatcgccga ccctggtggc gcgctccacc gcgccttgtg cgtattccac
      601 gggaaagctg tcccgggaaa ctggtggcac ccccagtgcc gccaggcccg ggaacgtgat
      661 cccgaggttg acacagtatt gtggcttagt ctcccaaggc tgcgcggtgg tgacggacac
      721 ggcagcatcg ctggtgatga gactgccgag gaaggcgcgc gcagcccgcg gctcgcgcac
      781 agccaggccg atatatcgcg ccgctggcat cgtgtagccg cgcaggatga gaccctgcac
      841 atctgccagg tcgaccaccg gcatcgcagt atctcctcct tcaggtcatc agcgcgatgc
      901 ggtggccgca ctgagtatgt cgagtactgt ccgatcgggg tatgcgctgt aaaacgttcc
      961 cacacagcga aagtcgtggg cacggatata ctccaggaac tcttggcgat aagtttgcac
     1021 cggcagcggc ggtccgtctt cgacgtgctc caagagcatg ttgaagacgt cgccgatttc
     1081 gttgatgaac tcgttgaagt aaacctcgaa gtcaccatcg taggaggtga tcaccgcgag
     1141 ttggtcgttg tccaaaaacg tgaacctggc gaagtgcacg gtaacgatct tattcagcgc
     1201 tatcgtgacc gggttcttgt ccggtggcaa ggactgcaga tgctcgacgg tctttcgaag
     1261 ggctgcgtag tcatctggag acttcgcctt catcaccatc gtcagaggat tctggacggg
     1321 cggcgacggt ccggtcatgg catctccttc cggttggcgg cagcgccctc gtgcacagtg
     1381 gctcggttgt ctgtcggttg tcttgcatgt tggcctgcgt catgttgtcc tgcatcggtg
     1441 cctgccacag catgatcagg tcagcggcac tacctcatcg ccagccctgt acactgtaca
     1501 ttcacagtac gttgtcctcg ccgcgtacag tgtggagggt ggcggtgata ccgccatcgg
     1561 ccaaacgggt gtacacctgt ccgttgcata acttcag
//
LOCUS       DATADN010000533         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_668336, whole genome shotgun sequence.
ACCESSION   DATADN010000533 DATADN010000000
VERSION     DATADN010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668336"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VHH53_06920"
     CDS             complement(<1..182)
                     /locus_tag="VHH53_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289915.1"
                     /translation="MIILIVAGIGLLVLVAIVVGFVEATQAPTWRQIAAERRARWEAK
                     QPQLHGFDPYAAPPLRG"
     gene            668..>1679
                     /locus_tag="VHH53_06925"
     CDS             668..>1679
                     /locus_tag="VHH53_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2289916.1"
                     /translation="MRSAARRLSAACVAGALAVTLAACAESQRDPGTGEGGAGATGGT
                     MVFGAPGAPDNFDPLFAQDGETFRPARQIYDTLITYEQGSAELAPGLATSWTPSEDGT
                     VWTFTLREGVRFHDGTPFNAEAVCFNFNRWFNLPNAAAQSQAIYYYEVFGGYANNLIE
                     GVTDPVYNSCEAPNPTTAVVRLNRYKGAFPAAFGLTSLSISSPTALQQYNADQVEQSG
                     EAFTYPSYATEHPTGTGPFRFESYDRAAGTVTLVRNDQYWGEPAKLDRLIFRLIPDEN
                     ARRQELQAGTIDGYDLPSPADWQSLEDAGFQVLIRPVFNILYLGINKKNNPALADLRV
                     RQA"
BASE COUNT          263 a          585 c          571 g          260 t
ORIGIN      
        1 ccgcggagcg gcggagcggc gtacgggtcg aagccgtgca gctgcggctg cttggcctcc
       61 cagcgcgcgc gccgctccgc cgcgatctgc cgccaggtgg gggcctgtgt ggcctccacg
      121 aagccgacca cgatcgcgac caggacgaga agaccgatgc ctgcgacgat cagaatgatc
      181 atggcgacga cccgttccgg tcggcggtgc tggcgccggt gtgcttcccg agtgacatga
      241 gtgggctcct tcccgtgtgc tgctctcgcc ggcggtgtgc cggacggttg tgggtcccgg
      301 gctgccccgg gacgggggcg gttcctggtt cgcaggagct ccgcgatgag tgcagaacgg
      361 cggaggcggg tacctgagtg ttggtcacgg tggcctcctc ccgggatgcc cggctggccc
      421 cgcggcgctg cgggggtcgg ccggacactt agattgccgt acgcaaggtc ctcctgcaat
      481 tgcaaggctg gcgctcagca gacaatcacc cgactgccgc agcgcggcgg tgtccctgcg
      541 cgctgggccg gacgttgctt gatctgatcg ttaagtgccg tagcggaacc gtgacgcgat
      601 gacctaccgg tcggtgtctg aatcgcacta gtttccagcg ggtctcctat gaagaggtgt
      661 tgcccgcatg agatcagctg ccagaagact ctcggccgcg tgcgtggccg gcgcgctcgc
      721 cgtcaccctc gctgcgtgcg ccgagtctca gcgcgaccca ggtacgggag agggtggcgc
      781 cggcgccacc ggcggcacga tggtgttcgg agcgcccggc gcaccggaca acttcgaccc
      841 gctgttcgcc caggacggcg agacgttccg gccggcgcgg cagatctacg acacgctgat
      901 cacctacgag cagggcagcg ccgagctcgc gcccggcctg gccacctcgt ggacccccag
      961 cgaggacggc acggtatgga cgttcacgct ccgcgagggc gtgcgcttcc acgacggcac
     1021 gccgttcaac gccgaggcgg tctgcttcaa cttcaaccgc tggttcaacc tgcccaacgc
     1081 ggcggcgcag agccaggcga tctactacta cgaggtgttc ggcggctacg cgaacaacct
     1141 catcgaaggc gtcaccgacc cggtctacaa ctcctgcgag gcgccgaacc cgaccacggc
     1201 cgtggtgcgg ctgaaccggt acaagggcgc gttccccgcg gcgttcggcc tcacgtcgct
     1261 gtcgatctcc agccccaccg cgctgcagca gtacaacgcc gaccaggtcg agcagagtgg
     1321 tgaggcgttc acctacccct cgtacgccac cgagcacccc accggcaccg gcccgttccg
     1381 cttcgagagc tacgaccgtg cggccggcac cgtcacgctg gtccgcaacg accagtactg
     1441 gggtgagccg gccaagctcg accggctgat cttccgtctg atcccggacg agaacgcgcg
     1501 acgccaggag ctgcaggccg gcacgatcga cgggtacgac ctgccctcgc ctgccgactg
     1561 gcagtcgctg gaggacgcgg gcttccaggt cctgatccgg ccggtgttca acatcctcta
     1621 cctcggcatc aacaagaaga acaacccggc gctcgcggat ctccgggtgc gccaggcga
//
LOCUS       DATADN010000534         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_49408, whole genome shotgun sequence.
ACCESSION   DATADN010000534 DATADN010000000
VERSION     DATADN010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49408"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..726
                     /gene="rpsE"
                     /locus_tag="VHH53_06930"
     CDS             121..726
                     /gene="rpsE"
                     /locus_tag="VHH53_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX2289917.1"
                     /translation="MPGRMRREGGGERSDRRDRRDGGRGAAQQEKSPHLERVVAINRV
                     AKVVKGGRRFSFTALVVVGDGDGMVGVGYGKAKEVPAAIQKGVEEAKKNFFRVPRIAS
                     TIPHPITGEAAAGVVLLRPASPGTGVIAGGPVRAVLECAGVHDVLSKSLGSDNAINIV
                     HATVAALKGLQRPEEVAARRGLPLEDVAPAGMLRARAAQGA"
     gene            728..940
                     /gene="rpmD"
                     /locus_tag="VHH53_06935"
     CDS             728..940
                     /gene="rpmD"
                     /locus_tag="VHH53_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2289918.1"
                     /translation="MAQPDSTVRPSLRITQTRSTIGSLRNQRETLRSLGLRKIRQSVV
                     KEDNSQVRGMIATVRHLVAVEEVDSK"
     gene            937..1380
                     /gene="rplO"
                     /locus_tag="VHH53_06940"
     CDS             937..1380
                     /gene="rplO"
                     /locus_tag="VHH53_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX2289919.1"
                     /translation="MTIKVHHLRPAAGAKTAKVRVGRGGGSKGKTAGRGTKGTKARKN
                     VPASFEGGQMPIHMRLPKLKGFRNRFRTEYQVVNVGDLARLFPDGGTVGPEQLVAAGA
                     VRKGLPVKVLGNGDLGGVALQISAQAFSGSARDKISAAGGSSTEL"
     gene            1571..>2051
                     /locus_tag="VHH53_06945"
     CDS             1571..>2051
                     /locus_tag="VHH53_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2289920.1"
                     /translation="MLGAFRSALATPDLRRKILFTLGMVVIYRIGATLPSPGVSYVNI
                     QQCLDQVQRGDNQNIYSLINLFSGGALLQLAVFALGIMPYITASIIVQLLTVVIPRFE
                     QLKKEGQSGQSKLTQYTRYLTIALAVLQATGIVALAVRGQLFSDCALPPIPDESVFNL
                     "
BASE COUNT          360 a          624 c          715 g          352 t
ORIGIN      
        1 gccttcgaca gctctgctgg tgagttcggg agcagcaggg ctagcaagtc cgcagatctg
       61 cggagtatct acaaggcagc agcaacaaca gcagcacgcg agcaatggaa ggaacgcctg
      121 atgcctggac gcatgcggcg ggaaggcgga ggcgagcgca gcgatcgtcg ggaccgccgc
      181 gacgggggcc gaggtgcggc ccagcaggag aagagcccgc acctcgagcg cgtggtcgcg
      241 atcaaccggg tggccaaggt cgtcaaaggt ggccggcgct tcagctttac cgcgctggtc
      301 gtggtgggcg acggtgacgg catggtcggc gttggttacg ggaaggccaa agaggtgccg
      361 gcggcgatcc agaaaggcgt ggaggaagcc aagaagaatt ttttccgggt tcctcggatt
      421 gccagtacca tcccgcatcc gatcaccggc gaggctgcgg caggtgttgt gctgctccgc
      481 cctgcctctc ctggtaccgg ggtcatcgcg ggaggcccag tacgagcggt gctggaatgc
      541 gcaggtgtgc acgacgtgct gtccaagtca ctgggtagtg acaacgcgat caacatcgtg
      601 cacgccacag tggccgcgct gaagggtttg cagcgtcctg aggaggtggc cgcccggcgc
      661 ggcctgccgc tggaggacgt ggccccggcc ggcatgttgc gggcgcgtgc cgcacaggga
      721 gcgtgagatg gcacagccag attcgacggt acgaccgtcg ctgcggatca cccagacgcg
      781 cagcaccatc ggtagcctgc gcaaccagcg cgagaccctg cgttccctgg gactgcgcaa
      841 gatccggcag tctgtggtca aagaggacaa ctcccaggtt cgcggcatga tcgccaccgt
      901 ccgtcacctg gtggcggtgg aggaggtcga ctcgaaatga cgatcaaggt tcatcacctg
      961 cgtccggccg ctggcgcgaa gaccgccaag gtgcgggtcg gtcgcggcgg ggggtccaag
     1021 ggcaagaccg cagggcgcgg taccaaagga accaaggctc gtaagaatgt gcccgcgagt
     1081 ttcgaaggcg ggcagatgcc gattcacatg cggctgccca agctcaaagg tttccgcaac
     1141 cggttccgta ccgagtacca ggtcgtgaac gtcggtgact tggcccggtt gtttcctgac
     1201 ggcgggaccg tgggaccaga gcagctggtg gctgccggtg ctgtgcggaa gggattaccg
     1261 gtgaaggtac tcggcaacgg tgatctcggt ggggtggcgc tgcagatcag cgcccaggca
     1321 ttctctggtt ccgcccggga caagatttcg gcggcaggcg gcagcagcac cgagctgtag
     1381 gcggcgctgg cggcccctac gggctctgtt aatacagtcc ggcgctgctg ttacagtcgg
     1441 ccagagtgcg tgggcgagct ggctggcatg cgccgtcacg tgcccctgca tcgcccggtc
     1501 gccgcccctg gtgatgcgac cgccggtcca cgatcgacgg cctcactacg gccgtgccca
     1561 ggaggtagga gtgctcggcg ccttccgctc ggcccttgcg acgccggatc tgcgccgcaa
     1621 gatcctcttt accctcggca tggtggtgat ctaccggatc ggcgcgacgt tgccctctcc
     1681 gggggtctcc tacgtcaata tccagcagtg tctggaccag gtgcagcgtg gtgacaacca
     1741 gaacatctac tcgctgatca atctgttcag cggtggggcg ttgttacagc tggcggtttt
     1801 cgcgctaggg atcatgccct acatcaccgc gagcatcatc gttcaactgc tcaccgtcgt
     1861 gatccctcgc ttcgaacagc tcaagaagga ggggcagtcc ggccagagca agctcaccca
     1921 gtacacccgt tacctgacga tcgcgctggc tgtcctgcag gccaccggca tcgtggcgct
     1981 ggcggtgcgg ggtcagctgt tctcggactg cgcgctgccg cccatccccg acgaatcggt
     2041 gttcaacctg g
//
LOCUS       DATADN010000535         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_977457, whole genome shotgun sequence.
ACCESSION   DATADN010000535 DATADN010000000
VERSION     DATADN010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977457"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1192
                     /locus_tag="VHH53_06950"
     CDS             209..1192
                     /locus_tag="VHH53_06950"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289921.1"
                     /translation="MRRPTVVRALLALALVILSGYIALSSAPRLGLDLSGGTQIVLET
                     RDAPGIEANAESTDRALEVLRERVDALGVAEPSLSRSGETRIIIELPGVTEPAEAAAV
                     IGRTAQLSMHPVLPPGPAPDQPQSQRQLPDEAGIPLPLAPAAITGDDVDGASASFDAQ
                     NVRGWLVNVEFADGEAWRQLTAQAACAPVGDPSRRIAIVLDDRIISSPQVDPSVGCEA
                     GISGGSTQITGSFTQDEATDLAVLIEGGALPIPVEIIEQRTVGATLGAEAIEASATAA
                     LIGIALTGVFLLAVYRLIGAVAVLALGAYAVISYAALTALGATLTPRGRAG"
     gene            complement(1167..>2204)
                     /locus_tag="VHH53_06955"
     CDS             complement(1167..>2204)
                     /locus_tag="VHH53_06955"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX2289922.1"
                     /translation="WASKGTTSEHRSLTPSTAAPRTTVRRPTVVRALLALALVILSGY
                     IALSSAPRLGLDLSGGTQIVLETRDAPGIEANAESTDRALEVLRERVDALGVAEPSLS
                     RSGETRIIVELPGVTEPAEAAAVIGRTAQLSMHPVLPPGAPVQRQLPDEAGIPLPLGP
                     AAITGDDVDGASAAFDAQNVRGWLVNVDFADGEAWRQLTAQAACAPVGDPSRRIAIVL
                     DDRIISSPQVDPSVGCGGGIPGGSTQITGSFTQDEATDLAVLIEGGALPIPVEIIEQR
                     TVGATLGAEAIEASATAALIGIALTGLFLLAVYRLIGAVAVLALGAYAVISYAALTAL
                     GATLTLPGLAG"
BASE COUNT          323 a          802 c          766 g          313 t
ORIGIN      
        1 cccgcgatcc gtcacctcac cgctccgacg ctattcggcc tgctagtagg cgaagctgca
       61 ccgtcaccaa ccacgggtca gtcgtctggt ggcgcgcatc cacggcacca cggaccggcc
      121 gcgcagcagg ccggtcctgg gcaggtaagg gcaccaccag cgaccaccga tcgctgaccc
      181 ccatcgactg ctgccccgag gactacccgt gagacgcccc accgtggtgc gcgccctgct
      241 ggcgctcgcc cttgtcatac tgtccggcta catcgccttg agttccgcgc cgcgcctcgg
      301 gctggacctc agcggtggca cgcagatcgt cctggagacg cgcgacgcac ccggtatcga
      361 ggcgaacgcg gagtctaccg accgcgcact cgaggtgctg cgcgagcgcg tggacgcgct
      421 cggtgtcgcc gagccgtcgc tgtcccgctc tggtgagacc cggatcatca tcgagctgcc
      481 cggggtcacc gagccggccg aggcggccgc ggtgatcggc cgcaccgcgc agctgtccat
      541 gcaccccgta ctgcctcccg gacccgcgcc cgaccaaccg caatcgcagc ggcagctccc
      601 ggacgaggcc gggatcccgc tgccgctggc tccggccgcg atcaccggcg acgacgtcga
      661 cggcgcgagc gcctctttcg acgcacagaa cgtgcgcggc tggctggtga acgtcgagtt
      721 cgccgacggt gaggcgtggc ggcaactcac cgcgcaggcc gcatgcgccc cagtaggtga
      781 tccgtcccgg cggatcgcga tcgtgctgga cgaccggatc atctcctctc cgcaggtcga
      841 tccgtcggtc ggctgcgagg ccggcatctc cggcggcagc acccagatca ccgggtcgtt
      901 cacccaggac gaggcgaccg accttgcggt gctgatcgag ggcggcgcgc tgccgatccc
      961 ggtggaaatc atcgagcagc gcaccgtcgg agccaccctg ggcgccgagg ccatcgaggc
     1021 cagcgccacc gccgccctga tcggtatcgc cctgactggg gtcttcctgc tcgcggtcta
     1081 ccgtctgatc ggcgcggtgg cggtgctcgc actcggtgcc tacgcggtga tctcttacgc
     1141 cgcgctcacc gcgctgggcg ccaccctcac cccgcgaggc cgggcagggt gagggtggcg
     1201 cccagcgcgg tgagtgcggc gtaagagatc accgcgtagg caccgagtgc gagcaccgcc
     1261 accgcgccga tcagccggta caccgcgagt aggaagagcc cggtcagggc gataccgatc
     1321 agcgcggcgg tggcgctcgc ctcgatggcc tcggcgccca gggtggcccc gacggtgcgc
     1381 tgctcgatga tttccaccgg gatcggcagc gcgccgccct cgatcagcac cgcgaggtca
     1441 gtcgcctcat cctgggtgaa cgacccggtg atctgggtgc tgccgccggg gatgccaccc
     1501 ccacagccga ccgacggatc gacctgcggg gaggagatga tccggtcgtc cagcacgatc
     1561 gcgatccgcc gggatggatc acctactggg gcgcatgccg cctgcgcggt gagttgccgc
     1621 caggcctcac cgtcggcgaa gtccacgttc accagccagc cgcgcacgtt ttgcgcgtca
     1681 aaagccgcac tcgcgccatc gacgtcgtcg ccggtgatcg cggccggacc caacggcagc
     1741 gggatcccgg cctcgtccgg cagctgtcgc tgcaccggcg cgccgggagg caatacggga
     1801 tgcatggaca gctgcgcggt gcggccgatc accgcagcgg cctcggccgg ctcggtgacc
     1861 ccgggtagct cgacgatgat gcgggtctca ccagagcggg acagcgacgg ctccgcgaca
     1921 ccgagcgcgt cgacgcgctc gcgcagcacc tccagtgcgc ggtcggtgga ctccgcgttc
     1981 gcctcgatgc cgggggcgtc gcgggtctcc aggacgatct gcgtgccacc gctgaggtcc
     2041 agcccgaggc gcggcgcgga actcagggcg atgtagccgg acaggatgac aagggcgagt
     2101 gccagcaggg cgcgcaccac ggtggggcgt ctcacggtag tcctcggggc agcagtcgat
     2161 ggtgtcagcg atcggtgctc gctggtggtg cccttacttg ccca
//
LOCUS       DATADN010000536         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_375052, whole genome shotgun sequence.
ACCESSION   DATADN010000536 DATADN010000000
VERSION     DATADN010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375052"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..556)
                     /locus_tag="VHH53_06960"
     CDS             complement(308..556)
                     /locus_tag="VHH53_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289923.1"
                     /translation="MDTMTPSPNVAAWQASASEPGSEPSEPTLSGQLRAIVVLLSMIL
                     FVVTAQFGYGLYRQAQIRHCTDVEASSAVQQAACLATH"
     gene            593..1288
                     /locus_tag="VHH53_06965"
     CDS             593..1288
                     /locus_tag="VHH53_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289924.1"
                     /translation="MAWLLPRYLPAGLTIPIKISPGFLGSSVGVSGLVRERFGCWESV
                     ANRGRACRSAPLVVLASPQARHTRTSAAYSVSVEGPPGERPRPVGDTGLDDYDRAVFK
                     NFPWRKRSGAALLFAVAVAAFPAQPAAAGGTYRYMSTYVFCQGGSSPSWGSDAYSDKP
                     EMHLDLVFAGLDGSTWVTLAHSQHNRWTRIRGKFFVIEVRKGAKYGDRYKRYRVTGKL
                     TRGNSWTSDMDEC"
     gene            1282..1737
                     /locus_tag="VHH53_06970"
     CDS             1282..1737
                     /locus_tag="VHH53_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289925.1"
                     /translation="MLTRRRPRPALVAAAALAMMVLPAAPLHAEDVELQGVCGQSGKK
                     LEYWAEAKRRHSANGALTVTISADFEGRGWQTIAEHRDSEPTRTASGDAGGGPQIAVF
                     VWTVPDPGMDGAFRRTQKTDKPPAKGYRMRLLFDAPDAHLEDTTHCNNR"
     gene            1755..1973
                     /locus_tag="VHH53_06975"
     CDS             1755..1973
                     /locus_tag="VHH53_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289926.1"
                     /translation="MRRAATYEPSSPGAFSVLLAEIEAVDADAAWTSVSDDPAELAES
                     QSLRRQADALRRALADLDRQIASALDRQ"
BASE COUNT          363 a          699 c          742 g          311 t
ORIGIN      
        1 ggcatcgaag gcgaatggca gctggccgcc ctggcggagt aacgccagca ccctgagcga
       61 cggtggcacg gtgtcctttc gaggttcggg gtggaccggg ttcgtgccgc cagtcccgac
      121 ccggcaacga gcggtgggcg ccaaaccagc gttcctgtga aacctgcacc ccctgagcgc
      181 gttcggtcag gtctgcgggg caaccaccca tcggacgaac cgctactgcg gaagctgcgg
      241 ccaggcgcga caaggccccc ggggctggat ccgggggcct tgcttcagct ggtgaaactt
      301 gcggtgtcta gtgggtcgcc aggcacgcgg cctgctgtac tgcgctcgac gcctcgacgt
      361 cggtgcagtg acggatctgg gcctgccggt agaggccgta gccgaactgc gcggtgacga
      421 cgaagaggat catcgacagc aggaccacga tggcccgcag ctgcccggac agggtgggtt
      481 cggaaggctc cgaaccaggc tcgctggcgg aggcctgcca ggcggccacg ttgggggaag
      541 gggtcatggt gtccatgcgg aattgctcct gcctcattct cggtgaacgt caatggcatg
      601 gctgctgccg cgatatctcc cggcaggtct caccatcccg atcaaaatct cgccgggctt
      661 tttaggttct tcggtcggcg tgagcggatt ggtgagggaa cggttcggtt gttgggaaag
      721 cgttgcgaat cgcggccgcg cctgccggtc agcgcccctg gtggtgttgg cctcgccgca
      781 ggcccggcac acccggacct cggcggctta cagcgtcagc gtcgaaggtc cacccggcga
      841 acgccctcgt cccgttggcg acacgggcct ggacgattac gatcgcgccg tgttcaagaa
      901 ctttccatgg aggaaacgga gcggggcggc gctcctgttc gcagtggcgg tggcggcgtt
      961 tcccgcccag ccggccgcgg cgggcggaac ctaccggtac atgagcacgt atgtgttctg
     1021 ccagggtggc tccagtccga gctggggctc cgacgcttac agcgacaagc ccgagatgca
     1081 cctcgacctg gtcttcgccg gattggacgg cagcacatgg gtcaccctcg cccacagtca
     1141 acacaacaga tggacccgca tccggggcaa gttcttcgtc atcgaggtcc gcaagggcgc
     1201 caaatacggg gaccgataca agcggtatcg agtcacgggg aaactgacgc ggggtaacag
     1261 ctggacgagc gacatggacg agtgttgaca cgccgccgcc cgcgcccggc gctggtcgcc
     1321 gccgccgcgc tggccatgat ggtgctcccc gccgcgccgc tccacgccga ggacgtggag
     1381 ttgcaaggcg tctgtggcca gtcgggcaaa aaactcgagt actgggccga agcgaagcgc
     1441 cgccacagtg ccaacggagc gctgacggtc accatcagcg cagacttcga aggccggggc
     1501 tggcagacga tcgccgagca ccgcgacagc gagccgacga ggaccgcatc gggcgacgcc
     1561 ggcggcggcc cccagatagc ggtcttcgtc tggaccgtgc ctgatccggg catggacggc
     1621 gcattccgac ggacccagaa gacagacaag cctccggcca agggctaccg gatgaggctg
     1681 ctcttcgacg cccccgatgc gcacctcgaa gacacgacac actgcaacaa ccggtagacc
     1741 gcggtctctt ccaggtgcgc cgcgcggcga cctacgaacc cagctccccc ggggcctttt
     1801 ccgtactgct ggcggagatc gaggccgtcg acgccgacgc ggcgtggacg tcggtgtcgg
     1861 acgaccccgc cgagctcgcc gaatcgcagt ccctgcgacg ccaggccgac gccctgcgac
     1921 gggccctggc cgacctggac cgccagatcg cgtcggcgct cgaccggcag taacagcggc
     1981 gcgccgagaa atggcgcgaa taccgcggtg agggaggacg ccggggtggt gctgcggggc
     2041 cggcgatccc ggcgtgccga ttggcgacgg agcggcggac gacacccgcg gctcatgggg
     2101 acaccccatg acccc
//
LOCUS       DATADN010000537         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_977691, whole genome shotgun sequence.
ACCESSION   DATADN010000537 DATADN010000000
VERSION     DATADN010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977691"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VHH53_06980"
     CDS             <1..763
                     /locus_tag="VHH53_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX2289927.1"
                     /translation="LDVSDRAGKPRDRGLTHVLDKGATIPALEAWLIQTGHLVDVIKI
                     GWGIAYIDPVVKERVAVCHAAGVMVSLGGTLLEVAVAQDRLTELRRWAADIGVDALEV
                     SNGLGVMSSQRKAALIHELSGEFVVLAETGLKTGTIPVVADAWLAEMEADLAAGARWL
                     IAEGRESGTVGLYEADGSVRATLVEAIVARLPVERIIFEAPRTAQQSWMVRHLGADVS
                     LGNIPLEEVMPLETLRLGLRADTARLPDRDLKAAL"
     gene            760..>1545
                     /locus_tag="VHH53_06985"
     CDS             760..>1545
                     /locus_tag="VHH53_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289928.1"
                     /translation="MIPDPAPNTITRPYRGLSVQEVDVALNEQALTAFLLGREVYRRS
                     EYLVLRNGRQTALVAVRKQSEVPLFSPVVQARVLAGPEQVVSIEAPEVDVGNATALAR
                     VALAHARPGALAYVVTGRYEHVNFIWQPAPVRVRVTEVVPPIPPKLFTMAEQVVAFDE
                     DLPPIELVLEAVDIPELAAAHPAASYLLPCRGAGTELGAPVSYLDTRPPDRANWLMIG
                     CERSLQFHRHFYGDEPPRVDLCPRRRSASLDDLTLAKCCLLERG"
BASE COUNT          232 a          525 c          541 g          247 t
ORIGIN      
        1 cctcgatgtc tcggaccggg ccggtaaacc tcgcgatcgc ggcctgaccc acgtactgga
       61 caagggcgcc acgatcccgg ccttggaagc gtggctgatc cagaccggtc acctcgtcga
      121 cgtcatcaag atcggctggg gcatcgccta catcgacccg gtggtcaagg agcgggtcgc
      181 ggtgtgccac gcggccggtg tgatggtgtc gctgggcggc acgcttctgg aggtggccgt
      241 cgctcaggac cgcctcaccg agttgcggcg ctgggccgcc gacatcggag tggacgccct
      301 cgaagtgtcc aatggcttgg gcgtgatgag ctcccagcgc aaggcggcgc tgatccatga
      361 gctgtccggc gagttcgtcg tactggccga gaccgggctc aagaccggga cgatccccgt
      421 ggtggccgac gcatggctcg ctgagatgga ggcagacctc gccgccgggg cccgctggct
      481 catcgcggaa gggagagaaa gcggaacggt cgggttgtat gaggccgacg ggagcgtgcg
      541 ggccacgctg gtcgaggcga tcgtcgcccg cctgcccgtc gaacggatca tcttcgaggc
      601 gccgcggacc gcccagcagt cctggatggt ccgtcacctc ggtgccgacg tcagcctggg
      661 caacatcccg ctcgaggagg taatgccgtt ggagacgctg cggctcggtc tgcgggccga
      721 caccgcgcgg ctgcctgacc gcgacctcaa ggcggcgctg tgatccctga cccagcaccg
      781 aacaccatca cccgccctta ccgtgggctg tcggtgcagg aggtcgacgt cgcactcaac
      841 gagcaggccc tgacggcctt cctgctggga cgggaggtct accggcgcag cgaatacctc
      901 gtgctgcgca acgggaggca gaccgccctg gtggcggtcc ggaagcagtc ggaagtaccg
      961 ctgttctcgc cggtggtgca ggcccgggtg ctggccggcc cggagcaggt ggtgtccatc
     1021 gaggcaccgg aggtcgatgt cggcaacgcc accgccctgg cacgggtggc gctcgcccat
     1081 gcccggccag gcgcgttggc ttacgtggtc accgggcgct acgagcacgt caacttcatc
     1141 tggcaacctg cgccggtgcg ggttcgggtg accgaggtgg tgcctcccat cccaccgaag
     1201 cttttcacga tggccgagca ggtggtcgcc ttcgacgagg acctgccccc gatcgagttg
     1261 gtgctcgaag cggtggacat ccctgagctg gccgcggccc accccgccgc cagctacctg
     1321 ctgccctgcc gcggcgccgg caccgaactc ggcgcaccgg tgtcctacct cgacactcgg
     1381 ccacccgatc gagccaactg gctgatgatc ggctgcgagc gatcgttgca gttccaccgc
     1441 cacttctacg gcgacgagcc accccgggtc gacctctgcc cgcgccgccg gtcagcctcg
     1501 ttggatgacc tgaccttggc gaagtgctgc ttgctggagc gggga
//
LOCUS       DATADN010000538         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_977704, whole genome shotgun sequence.
ACCESSION   DATADN010000538 DATADN010000000
VERSION     DATADN010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977704"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..359
                     /locus_tag="VHH53_06990"
     CDS             219..359
                     /locus_tag="VHH53_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289929.1"
                     /translation="MLADGLSHRGQASTDRIGSGLAQRPAGALPVHASWLDQIEIYFS
                     IP"
     gene            971..2242
                     /locus_tag="VHH53_06995"
     CDS             971..2242
                     /locus_tag="VHH53_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2289930.1"
                     /translation="MDASDESMQLVERVAALDLGKAGLTACVRVPHEDRPGRRRQEVR
                     EYSTLSRSLLELADWLRCERIELVAMEATSDYWKPVFYLLEAEGFTCWLLNAKHVKNV
                     PGRPKTDKIDAVWLAKIVEWGLCTPSLVHPKPIRQLRDLTRYRRSLIRERTREKQRAE
                     KLLEDAQIKLSSMISDIFGVSGRAMLEALIAGQRSPKALAEMACGKMRSKIAVLEEAL
                     TGHFEDHHGFLLQSMLSHIDSLNAQIDQVTTRIEAVIAPFTPAVARVDEIPGVGVVAA
                     QELIAEIGVDISRFPTPAHLVSWAKFAPIDNNSAGKKRGGSTGKGNPWLGGTLGEVVA
                     GISRTNTFLGERYRRLARSRGKKRAIVAVGNSVLTIIWHLLADAEAHYQDLGPDYYQS
                     KINARRRERELVRQLERLTGKKVTLKPAAAA"
BASE COUNT          434 a          764 c          734 g          430 t
ORIGIN      
        1 ggccggcttt ccccggcata cggctcatct gatcaataag tcgtcgaacc gggaaagccg
       61 gtcaacgggt cccaccccca gtggggcagt gtcgccctca gatccacagg tcagcggttc
      121 tccttgcacc acatcactcc gcagtgggtt cccatcaagg tccggatcta atcctggatt
      181 cattgcagcg agccggccag ctggaccaag agacaggggt gctcgctgac ggcttgtcgc
      241 atcgcggaca ggcctcgacc gaccggattg gcagcggtct ggcccaacgc ccagctggtg
      301 cactgccggt gcatgcgtcc tggttggacc agatcgagat ctacttctcc atcccttagc
      361 gcaaggtcct gcccccaaca acctgaccgc cctcgacgcc gtcgccgaac gggtgccggc
      421 ctttcaagac cgctacaaca ccaccgcgca accgttcgac tggagattca cccgtactga
      481 ccctgacccg actgctcgac cgcatcacgc acatcgcacc gccaccatga tctgtgagtg
      541 tcgttgcggt gctgctgggt ggaggaccac caggctcggt gtcggtgacc gcgttgtgac
      601 tcgaccatga tttggccgct gtggtgtgcc cttggcctga cttgtccgtc gtggttgccg
      661 gcgccggcct ggcccaccag ttggcttgat caaaagcatg tccgcccgag ggcgtgaccc
      721 gtcacagata tgtcgtgagt gccctagctg gggcgcccgt ggcgggcgtg aaaattcggc
      781 ctgtggccgc cggtgacgtc gtagttgctt ggtgcctggt cgaggccgca gatatgtctg
      841 acgctgggag ctgctgtgag gggccctgca ttgtcgcttc gagcacgaga gtccgcctct
      901 gttacctgcc cgctggttcc cccggcggcc acgcctatcg atccgaggcg agagtgggtg
      961 ggtaagtggc atggatgcat ccgatgagtc gatgcagttg gtcgaacggg tagcggcgct
     1021 ggatttgggc aaggccggct tgaccgcgtg tgtgcgggtg ccgcacgagg accggccagg
     1081 ccggcgtcgc caggaggtcc gcgagtactc cacgctgtcg cgatcgctgc tggagctggc
     1141 ggactggttg cgctgcgagc ggatcgagct ggtggccatg gaagcgacca gcgattactg
     1201 gaagccggtg ttttatctgc tggaggccga ggggttcacc tgctggctgc tcaacgccaa
     1261 gcacgtcaaa aacgtcccgg gcagacccaa aaccgacaag atcgacgccg tgtggctagc
     1321 caagatcgtt gaatgggggc tgtgcacgcc cagcctggtg caccccaagc cgatccggca
     1381 gctgcgggac ctaactcgct accgccgctc gttaattcgc gagcgcaccc gggaaaagca
     1441 gcgggccgaa aagctgctcg aggacgccca gatcaagctc tcctcgatga tcagcgacat
     1501 cttcggggtg tccgggcgag cgatgcttga ggcgttaatc gctggccaac gaagcccgaa
     1561 ggccctggca gaaatggcct gcggcaagat gcgttccaag attgctgtgc tggaagaggc
     1621 attgaccggt cacttcgagg accaccacgg cttcctgctg cagtcgatgc tgtcccacat
     1681 cgactcgttg aacgcccaga tcgaccaggt cacgacgcgg atcgaggcgg tgatcgcccc
     1741 ttttaccccg gcggtggccc gcgtggatga gatccccgga gtcggtgtcg tcgccgccca
     1801 ggaactcatc gctgagatcg gtgtggacat cagccgattt cctaccccag cacacttggt
     1861 ctcgtgggcc aagttcgccc cgatcgacaa caactccgcc ggcaagaagc gaggcggttc
     1921 caccggcaag ggcaatccgt ggttgggcgg cactctcggc gaagtcgtcg ccggcatctc
     1981 gcgcaccaac accttcctcg gcgagcgtta ccggcgcttg gcccgtagcc gaggtaagaa
     2041 acgggccatc gtcgcggtcg gcaactcagt gctcacgatc atttggcatc tcctggccga
     2101 cgctgaggct cactaccaag acctgggacc ggactactac caaagcaaaa tcaatgctcg
     2161 gcgtcgggaa cgtgagctcg tccgccagct ggaacgcctc accggcaaaa aggtcaccct
     2221 gaaacctgcg gcagccgcct aacgcagtcc agccacaaca cccagcaact cacctcaacc
     2281 cgggccgtgc ggttagcggg tacaaaagcc tgaccgtccc cgactccatt ttcgagtctg
     2341 ccactgcggt gacccttgcc ca
//
LOCUS       DATADN010000539         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_206166, whole genome shotgun sequence.
ACCESSION   DATADN010000539 DATADN010000000
VERSION     DATADN010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206166"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VHH53_07000"
     CDS             complement(<1..367)
                     /locus_tag="VHH53_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2289931.1"
                     /translation="MATVSISGLSLAPIEPSDEAALRQWYELRCSVAREDLPDDPPPC
                     RVHEFGRFRYPWPGEIETGWLARDGGSVVGGCVLYLPTLDNLHNAAGEILVAPKHRRQ
                     GIGRALLDHLRAEATRQGRI"
     gene            complement(428..1462)
                     /locus_tag="VHH53_07005"
     CDS             complement(428..1462)
                     /locus_tag="VHH53_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX2289932.1"
                     /translation="MTDRPPWPPGTATGIGSMPGTDALEAARTVIGELPDLPHLPELP
                     DRGAGADLIGRTAALLVDLAVELVPSGYRVTARPGGDHRRGVDLLRTDLDALEEAVQV
                     AGARLEVVKVQAAGPWTLAAEVELRSGHKILTDHGALREFAASLAEGLRAHVTEVSSR
                     LGTKTVVQLDEPGLPAVLAGSVPTPSGYGTVPAVPDSAAVELLRGVLDALPVPRIVHC
                     CAPRPPLAVLRSAGADALAVDAELVAGAPRATVDALGETWDCGASVLLGLVPAMEPAV
                     RPTVTDLARPALALVDRLGFDRALLAQHCLPTPSCGLAGATPGWARRALTLARELGQA
                     FVDPPDSWPS"
     gene            1693..1929
                     /locus_tag="VHH53_07010"
     CDS             1693..1929
                     /locus_tag="VHH53_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289933.1"
                     /translation="MSDTVSFAELDRQHVELLPARTVLSMFNAQDGGGLNGILDGIFP
                     KEGPLGSLLGMAPTDGNTGGNSADGADGAAGASQ"
     gene            complement(2020..>2124)
                     /locus_tag="VHH53_07015"
     CDS             complement(2020..>2124)
                     /locus_tag="VHH53_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HEX2289934.1"
                     /translation="QPLRGVAPGQAVALYRPDVAGDVVLGSATITATG"
BASE COUNT          351 a          735 c          723 g          315 t
ORIGIN      
        1 ggatccgacc ctgcctggtg gcctcagccc gtaagtgatc gagcaacgcc cggccgatac
       61 cctgccgccg gtgtttgggg gcgaccagga tctcgcctgc ggcgttgtgc aggttgtcca
      121 gagtcgggag gtacagcaca catccaccga ccaccgagcc gccgtcgcgg gctagccacc
      181 cggtctcgat ctcgccgggc cacgggtacc ggaagcgacc gaactcgtgt acccggcacg
      241 gtggtgggtc atctggcagg tcctcgcgcg ccactgagca gcgcagctcg taccactgcc
      301 ggagagccgc ctcgtcggac ggctcgatcg gcgcaagaga caacccgctg atgctgaccg
      361 tggccatgct cgccattccg ccacggccgt acggcccgcg ccaccgaatt tcgctgtcac
      421 cgcgacgtca cgaaggccag gagtcgggcg ggtcgacgaa tgcctgtccc agctcccggg
      481 cgagggtcag cgcccgccgc gcccaacctg gggtcgcgcc ggccagcccg caggacgggg
      541 tgggtaggca gtgctgggcg agtaacgccc ggtcgaagcc gagcctatcc accagagcca
      601 gcgcgggccg ggccaggtca gtgacagtgg gccgcaccgc gggctccatg gcgggcacca
      661 gcccgagcag taccgaggcc ccgcagtccc atgtctcgcc cagagcatcg acggtagccc
      721 gcggcgcacc ggcgaccagt tcggcgtcga cggccagcgc gtcggccccg gcgctgcgca
      781 gcacagccag cggtggccgc ggagcgcagc agtgcacgat tcgcggcact ggcagcgcgt
      841 cgagcactcc gcgcagcagt tcgactgccg cgctgtcggg cactgcaggc acggtgccgt
      901 accccgacgg ggtgggcacg ctgccggcga gcaccgcagg cagccccggt tcgtccagct
      961 gcacgaccgt tttcgtcccc agccggctgg atacttccgt cacgtgcgct cgcagccctt
     1021 cggccagcga cgcggcgaac tcacgtagcg cgccgtgatc ggtaaggatc ttgtgcccac
     1081 tgcgcagctc cacctcggcg gccagcgtcc acggcccggc agcctgcacc ttgaccacct
     1141 ccaggcgggc cccggcgacc tggaccgcct cctcgagcgc atccagatcc gtgcgcaaca
     1201 gatcaacgcc acggcgatgg tcgccaccgg gccgcgcggt gacccggtag ccggagggca
     1261 ccaactcgac ggcgaggtcg acgagcaacg ccgcggtgcg gccgatcaga tccgccccgg
     1321 caccccgatc aggtagctcc ggcaggtgcg gtaggtcggg gagctcgccg atcacggtgc
     1381 gggcggcctc aagggcatcg gtgccgggca tcgagccgat cccggtggcg gtaccgggag
     1441 gccagggagg tcggtcagtc accgggtctt tttatcagat acgggggtag ggaatctccg
     1501 tagcatgtcg ccgctcacta cgtattcgaa aagcaactcc acagctaggt aacccattca
     1561 gaagaattac gtgcgactac tgattgccac cggacatttc tattggcacg gtatgtgatg
     1621 gcgaatcagc aatgtaaatg cttaggtatt ttcgtccggg accacctcga caaccttcgc
     1681 taggaggatt agatgtctga cacagtgagc ttcgccgagc tcgaccggca gcacgtggaa
     1741 ctgctgccgg cccgcaccgt cctatcaatg ttcaacgccc aagacggtgg cggcctcaat
     1801 ggcatcctgg acggcatctt ccccaaggag gggcccctgg gcagcctcct tggtatggcg
     1861 ccgacggacg gcaacactgg cggcaacagc gctgacggcg ctgacggcgc tgctggcgcc
     1921 agccaatagc cgagtccact tgcgccgctg agggtgcggt gttgttggcg gacgtgggag
     1981 gggctgcgtt ccggccggtc ccctcccctc cgtggtagct cagccggtag cggtgatggt
     2041 ggcgctgccg aggacaacgt cgccggcaac atcgggacgg tagagcgcga cggcctggcc
     2101 gggtgccact ccgcgtagcg gctg
//
LOCUS       DATADN010000540         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1428499, whole genome shotgun sequence.
ACCESSION   DATADN010000540 DATADN010000000
VERSION     DATADN010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428499"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VHH53_07020"
     CDS             <1..187
                     /locus_tag="VHH53_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289935.1"
                     /translation="NHLLALMVQLKYFNRMGYFPRLDDVPDAVAHTSAQISGWARTSP
                     RCTTRTGPGPSSDADPR"
     gene            complement(306..1208)
                     /locus_tag="VHH53_07025"
     CDS             complement(306..1208)
                     /locus_tag="VHH53_07025"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2289936.1"
                     /translation="MAPDTVSLRYFLVLAQELNFTRAAARIGIAQPALSARMRRLEAE
                     LGTALLVRNTRSVVLTTAGAALAESAPPALAALDRAWDTARSAAAGELGTLRIGYSLS
                     AGAETAPALVDRLIRGNSGLEVGAVPMATPEISPAVADGRIDAGITRGEQPGRGVRRF
                     LLRRARIGVQLAQHHPLAEHPEIEIADAAAYPLRLPDRAANPVIHDQLSALFRDTRPH
                     PRFHTPAVSFDMSQRDLRDGVTLAPAGEAAATAQPAGLTWRPLRGAPSLTIHLVLPRE
                     QSPLHRRIRAVAKTLAHELHWLPD"
     gene            1397..1693
                     /locus_tag="VHH53_07030"
     CDS             1397..1693
                     /locus_tag="VHH53_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX2289937.1"
                     /translation="MAKGYWVSVYPAISDPERLTAYNELAGAAVQAGGGRVLSLLGGR
                     VVAHEAGITQRVVLIEFDSFEQAVAAYESEAYQKALVALPDGVERDFRIIDGID"
BASE COUNT          322 a          669 c          666 g          343 t
ORIGIN      
        1 caaccacctg ctggcactga tggtgcagct gaagtacttc aaccggatgg gctatttccc
       61 ccggctggat gacgtcccgg atgcagtggc ccacacatcc gcccagatct cgggctgggc
      121 gaggacgtcg ccgcggtgta cgactcggac cggaccgggg ccatcatcgg atgctgatcc
      181 gcggtagcga gttcgtgtcg gacattcagc aagaccgccg tcgtcgtcat caccgcgaag
      241 tccgccgggc gcaaggtctc cggcctcgcc accacaggcg tccgtctcgc ttgacctctt
      301 gcgcgtcagt ccggcagcca gtgcagctcg tgcgccaggg ttttggcgac ggcacggatg
      361 cggcggtgta gtggtgactg ctcgcgtggg aggaccagat ggatcgtcag gctgggcgcg
      421 cctcgcagtg gtcgccaggt gagaccggcc ggttgtgccg tggccgcggc ttctccggca
      481 ggggcgaggg tgaccccgtc gcgcaggtcg cgctgagaca tgtcgaaaga gactgcgggc
      541 gtgtggaatc gggggtgtgg tcgggtgtct cggaacagtg cggacagctg atcgtggatc
      601 acggggttgg ccgcacggtc cgggagtcgc agcggatacg cggccgcgtc ggcgatctcg
      661 atctccgggt gttcggccag cggatggtgc tgcgccagct ggaccccgat gcgcgcacgc
      721 cgcagcagga accggcgcac gccacggccc ggctgttcac cgcgggtgat cccggcatcg
      781 atgcggccgt cggcgaccgc gggggagatc tccggtgtcg ccatcgggac cgcgccgacc
      841 tcgagtccgc tgttgccgcg aatcagcctg tccaccaggg ccggtgccgt ctcggccccg
      901 gcgctgaggc tgtatccgat gcgcagcgtg cccagttcac cggccgccgc gctccgggcg
      961 gtgtcccatg cccggtccag cgccgccagc gcgggcggcg cggactccgc caaagccgca
     1021 ccggccgtgg tcaatacgac gctacgcgtg ttgcggacca gcagggccgt accgagttcc
     1081 gcctccaatc ggcgcatccg ggcgctgagt gcgggctgtg cgataccgat ccgtgcggcc
     1141 gcgcgggtga agttcaactc ctgcgccagc accaggaagt accgcaggct caccgtatcc
     1201 ggcgccacgt gccctccctg ccgattgata acgatccatt ctgagtctat cccgtaccgg
     1261 tctttccctc gaccgcatat caagccctaa cctgcgcgta tggcgattca actgacggca
     1321 gtacctgtcg ccgggatcga tcgatctgag tgtcaattcg aagtcggacg tgtccggccg
     1381 aacacaggag gtttccatgg ctaagggcta ctgggtcagt gtctaccccg ccatttccga
     1441 ccctgagagg ctgactgcct acaacgagct ggccggtgcg gctgtccagg ctgggggcgg
     1501 gcgcgtcctg tccctccttg gcggtcgagt cgtcgcccac gaggccggaa tcacgcaacg
     1561 cgtcgttctg atcgagttcg acagctttga acaggccgtc gcggcatacg agagcgaggc
     1621 ataccagaag gcgctggtgg ccctccccga cggcgtcgag cgcgacttcc gcatcatcga
     1681 cggcatcgac tgaccggcgg gcccagccat cgctgagcat ccgtcacctg atacgcacac
     1741 agtgctgaac gacgactcac gagacgagtt cgaagcgatg ccccgcttcc cagttcatcg
     1801 tcgacgtcct cgacggcctg atccctgtcg gcggctcgcc gcgctaccca acgtcgagca
     1861 acttgcctga cttgactgac acggcgatca agatctgacc tgccacaacg gcgattcacc
     1921 ctagataatg ccgtcggcaa cggaagagca ggtcccgagg gactatcgct ttgccggcat
     1981 gccgaggagt accggccctc
//
LOCUS       DATADN010000541         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_521969, whole genome shotgun sequence.
ACCESSION   DATADN010000541 DATADN010000000
VERSION     DATADN010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521969"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VHH53_07035"
     CDS             complement(<1..506)
                     /locus_tag="VHH53_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX2289938.1"
                     /translation="MAEVDGPVGTTRSTRNGPVSEEQLVQEGDVAGDYLERLLDLVDY
                     DGDIDLDVEAGRAMVSIDGGEDLERLVGPRGSVLEALQELTRLAVQQRTGARSRLMLD
                     IAQWRSTRRHELSKLGRSTAERVRDVGEPARLQPMTPFERKIVHDAVAAVDGVRSESE
                     GEEPQRRVV"
     gene            complement(622..1764)
                     /gene="yidC"
                     /locus_tag="VHH53_07040"
     CDS             complement(622..1764)
                     /gene="yidC"
                     /locus_tag="VHH53_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX2289939.1"
                     /translation="MLNFIYYPVSAILWFWHQAFGYLLGPSSGLGWTLAVVFLVFTLR
                     ALLFKPFVSQVRSMRKMQEFAPEIQKLKKKYGDDRQKMAAEMQKLQSQHGVNPLGGCL
                     PVLVQIPVFIGLFQVLRSFNRDFLTPEQNAQIGNYVFSAADVRSFLDADLFGVPLSSY
                     ITESTELLARYGDLSRWQIAAVAVPLTIVAGILTHLTAKHSVQRQNPAQAANPQAELM
                     NKLMVYLFPLGVVAGGPFFPIAILIYWLSNNLWTLAQQRWVYSRIDVEEAEKKETAIA
                     QRQSLAPRPGQKPVRPKKDAAGGRPAGAADFESGPESGSGAAQPSDVEPDSEVASNGA
                     ASTASNRDGSEASSNGKGTERTDSSTMPGMIQQRPRARKKTNRKRR"
     gene            complement(1758..2012)
                     /gene="yidD"
                     /locus_tag="VHH53_07045"
     CDS             complement(1758..2012)
                     /gene="yidD"
                     /locus_tag="VHH53_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX2289940.1"
                     /translation="MARVLVLLLQFYRRWISPFLPPTCRFHPTCSAYAVEALTVHGAL
                     RGSWLTARRLLRCGPWHPGGMDPVPTPRSPRSPAESESSC"
BASE COUNT          336 a          698 c          632 g          356 t
ORIGIN      
        1 acaacgcgcc gttgcggctc ctcgccctcg ctctcgctgc gcacaccgtc caccgccgcc
       61 accgcgtcgt gcacgatctt tcgctcaaac ggggtcatcg gttgtagccg agccggctct
      121 cccacatcgc ggacccgctc ggcggtcgag cgacccagct tgctgagctc atggcgccgc
      181 gtggaccgcc attgcgcgat gtcgagcatc aaccggctgc gggcaccggt ccgttgctgc
      241 accgctagtc gcgtcaactc ctggagggct tcgagcaccg agccacgagg ccccaccaac
      301 cgttccaggt cctccccacc gtcgatgctc accatggctc ggccagcctc gacgtcgagg
      361 tcgatatcgc cgtcgtagtc cactaaatca agcagccgct cgaggtagtc gcccgcgacg
      421 tcaccctcct gaacgagctg ttcctcgctc accggtccgt tgcgcgtcga ccgcgtcgtc
      481 ccgaccgggc catccacctc ggccacgccg tcagtgtctg cagtgtccgg aaccgcagtg
      541 tccggcacct cagcctcggt ccccgacgtc tcggttgcca gcgtctcggc cacacctgtc
      601 tcctctccgg ggatcgtgcc cttagcggcg cttccggtta gtcttcttcc gcgctcgggg
      661 ccgttgctgg atcatgccag gcattgtgga actgtccgtg cgctccgtgc ctttgccgtt
      721 ggaagatgct tcggatccgt cacggttcga tgctgtcgat gccgctccgt tcgaggcaac
      781 ctcgctgtcc ggttcgacgt cgctcggttg cgcagcgcct gagccggact ccggacctga
      841 ttcaaagtca gcagcacccg cgggacggcc accagcggcg tccttcttcg ggcggaccgg
      901 cttttgaccg ggtcgcggag ccagcgactg gcgctgcgct atcgccgtct ccttcttctc
      961 cgcctcttcg acgtcgatcc ggctgtagac ccaccgctgc tgagcgagcg tccacaggtt
     1021 gttggacagc cagtagatca ggatcgcgat cggaaagaac gggccaccgg caaccacgcc
     1081 cagcgggaac aggtagacca tgagcttgtt catgagctct gcctgcgggt tcgctgcctg
     1141 agctgggttt tgccgctgca ccgaatgctt ggctgtgagg tgcgtgagga tgccggcgac
     1201 gatcgtcagc gggacggcca ccgcggcgat ctgccagcgg gaaaggtccc cataccgggc
     1261 cagcagctcc gtggactcgg tgatgtagga cgacagcggc accccgaaca ggtcggcgtc
     1321 aaggaacgac cgcacatcgg ccgcgctgaa cacgtagttg ccgatctgcg cgttttgttc
     1381 gggggtcagg aagtcgcggt tgaacgaccg cagcacctgg aacaggccga tgaacaccgg
     1441 gatctgcacc agcacaggca ggcagccgcc cagcgggttc accccgtgct gcgactgcag
     1501 cttctgcatc tcggccgcca tcttctggcg gtcgtcgccg tacttcttct tcagcttctg
     1561 gatctcgggc gcgaactcct gcatcttgcg catcgaccgg acttggctga cgaacggctt
     1621 gaacagcaac gcacgcaggg tgaacaccag gaacaccacg gccagcgtcc acccgaggcc
     1681 actgcttgga cccagcaggt agccgaacgc ctgatgccag aaccacagga tggccgacac
     1741 cgggtagtag atgaagttca gcacgacgac tcactctcgg cgggggagcg gggtgatctc
     1801 ggtgtgggaa ccgggtccat ccctccaggg tgccagggtc cgcagcgcag tagccgtcgc
     1861 gcggtcaacc aggatccacg cagtgccccg tgaacggtca gtgcctcgac cgcataagcg
     1921 ctgcacgtgg ggtgaaaccg gcaggtcggc gggaggaacg gagagatcca ccggcggtag
     1981 aactgcagca agagcaccag cacccgggcc atcgggcgag cc
//
LOCUS       DATADN010000542         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_522009, whole genome shotgun sequence.
ACCESSION   DATADN010000542 DATADN010000000
VERSION     DATADN010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522009"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VHH53_07050"
     CDS             complement(<1..186)
                     /locus_tag="VHH53_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289941.1"
                     /translation="MPETLRVLVPWVIELFARIRMGRDPAQSTRTHPATTGATMTADL
                     HTTSPAIPLTIPPSAQDG"
     gene            complement(710..>1847)
                     /locus_tag="VHH53_07055"
     CDS             complement(710..>1847)
                     /locus_tag="VHH53_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2289942.1"
                     /translation="VARARRGLAGGHILEQTNGNSAFAHGCIVLMCADLDEVMPLLDA
                     SVAQAHRRGSIFALAGTKCFRALAWLWRGFLVEAEADARDAMQAFQTARIDMGIPYAA
                     VYLAGALMEQGRLTEAEAALDCVAPPSDRLQLGHWAWLLNRARLLLLQGRTEEGLEAM
                     LTYGRRLAVHGWHNPAISTWRSDAALALLALGRHDQAHALAAEELELSRRWGAPRALG
                     VSLRVAGLVEGGKEGLGLLRQAVDVLAPSSARLEHAKALVELGAALRRAGHRTEARQH
                     LRQGIDHAQLCAATPLVERGLTELRATGARPRRLRLSGPAALTPSERRVAELAATGRS
                     NRDIAQTLFITTHTVELHLTRAYRKLGITSRTHLTHTLPALNNS"
BASE COUNT          291 a          544 c          670 g          342 t
ORIGIN      
        1 gccgtcctgg gcgctcggcg gaatggtgag tgggatggcg ggtgaagtgg tgtgcaggtc
       61 agcggtcatg gttgctcccg tggttgccgg gtgggttcgg gtcgactgcg cgggatcacg
      121 tcccatgcgg atccgggcga acagttcgat gacccaaggg acgagcacgc gcagcgtctc
      181 cggcatctcg atgtcctccc ccgtccggcg gccgtccggc ggcccaaacg agtaaccagt
      241 ggtaggaggg cgagcaacgc ccgggagata cgtcatgatc aaccctgttg gtgtgacgtg
      301 gtgagcgaaa gtggccgtcg gtgctgactg agaaaccgtc gaacttattg tttggcggcg
      361 gattttgttg atctagaaga ggggcgaggt ctgggctgac agagatgagc aacgacgaca
      421 tagtaactct gagttaccat gtttctttct ctttctagcg ccacttggcg ctaggtagat
      481 ctgggagggt gctgcatcgg cctctccgct ccttttgggt cgtgacacta ttcatgctgc
      541 gccttggacc ggggttttcg cgttctgggt gccctcactg caccccccca atgttttggg
      601 cagtgggccc caacgttggt cggcctggtt gccttggagt cgcgacggtg gccgcgcgtc
      661 tttgtggaat ggccgtatca acatcggcca atgaatgatg ggtaagacgc tacgagttgt
      721 tgagtgcggg gagggtgtgg gtgaggtgtg tccggctggt gatgccgagt ttgcggtagg
      781 cccgggtgag gtgcagttcg acggtgtggg tggtgatgaa cagtgtttgg gcgatgtcgc
      841 ggttggagcg tccggtcgcg gcgagttcgg ccacgcggcg ctcgctggga gtcagcgccg
      901 ccgggcctga cagcctgagg cgtcgggggc gggcgccggt ggctcgcagc tcggtaagcc
      961 cccgctcgac taggggggtg gcggcgcata gctgggcgtg gtcgatgccc tggcgcaggt
     1021 gctgccgtgc ctcggtgcgg tggccggcgc ggcgcagcgc ggcgccgagt tcgaccagcg
     1081 ctttggcgtg ttccaggcgg gctgaggagg gggcaagcac gtccactgcc tggcggagca
     1141 gtccgagacc ttccttgccg ccctcgacga gcccagccac gcgcagcgat accccgagcg
     1201 ctcggggtgc tccccaacga cgggatagtt ccagctcctc ggcggctagc gcgtgggcct
     1261 ggtcgtgccg gccgagtgcc agcagcgcca gcgccgcgtc cgagcgccag gtactgatgg
     1321 ccgggttatg ccagccgtga accgccaagc ggcggccgta ggtcagcatc gcctctagac
     1381 cttcttcggt ccgtccctgg agcagcagca ggcgggcacg gttaaggagc catgcccagt
     1441 ggcccagttg aaggcggtca gatggcggag ccacgcagtc cagggctgcc tcggcctcgg
     1501 tcaggcgccc ctgctccatc aacgccccgg ctaggtagac agcggcgtac ggtatgccca
     1561 tgtcgatgcg ggcggtctga aacgcttgca tggcatcgcg ggcgtctgcc tccgcctcaa
     1621 ccaggaaacc acgccaaagc cacgcgagcg cccgaaagca cttcgtgccg gcaagggcga
     1681 agatcgagcc acgccgatgt gcctgcgcaa ctgaggcgtc caacaacggc atgacctcat
     1741 ctaggtcagc gcacatcagc actatgcaac cgtgggcaaa ggctgagttg ccattggtct
     1801 gctcaaggat gtggccaccg gcaaggcccc gccgggcccg agcgacc
//
LOCUS       DATADN010000543         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1259379, whole genome shotgun sequence.
ACCESSION   DATADN010000543 DATADN010000000
VERSION     DATADN010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259379"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1299
                     /locus_tag="VHH53_07060"
     CDS             91..1299
                     /locus_tag="VHH53_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2289943.1"
                     /translation="MKFVGWDWASTTHDITVLDQAGRILERWACEHTEAGLAQALHRL
                     RQHGQPATLPVIIERTSGLVIDRLLAAGHPVVPVHPTAFHAARPRWGASSAKSDPGDS
                     YKLADYLRTDGHRLRRLEPVDAGLRQLQSLVRLRDDHIAAKTAASNQLGALLDAHWPG
                     PKALFYRLGSEIALAFLTNYPTPESAARLGETRMAAFLRRHAYRGGKTPTELLTRLRL
                     APTPPTGLPPEVLTQLIGAQIQLLRTLLSTITALEAEIKRIVATHPRARLLQQLPGIG
                     QINLAQILAEIGPILDRVADAEQAAAECGATPVTKASGKTTGVYFRWAANTRARKAIT
                     AFAHNSRMQSRWAQGLYRDARNRGKRNPHANRIVARAWIRVIWACWHTNTPYDPHKHT
                     AEQRAAATLT"
BASE COUNT          298 a          602 c          406 g          219 t
ORIGIN      
        1 atgccccgat gagggccgaa caatgaggaa ccggcagcag ggcctcgaca cccgtggagt
       61 ccaccacgca ctccaaggag agaccagatc gtgaagtttg tcgggtggga ctgggccagc
      121 acgacacacg acatcaccgt ccttgaccag gccggacgca ttcttgagcg gtgggcctgt
      181 gagcacaccg aagcgggctt ggctcaggcg ctgcaccgtc tgcgccagca cggtcagccc
      241 gccaccctgc ccgtgatcat tgaacgaacc agcgggttgg tcatcgaccg gttgctcgcg
      301 gccggtcatc ccgtggtgcc ggtgcacccc acggccttcc acgctgcccg gccccgctgg
      361 ggcgcctcca gcgcgaagtc cgacccgggg gacagctaca aacttgccga ctacctgcgc
      421 accgacggcc accggctacg ccgcctggag cccgtcgatg ccggattacg ccagctgcaa
      481 agcctggtgc gactacgcga tgatcacatc gccgccaaaa ccgcagcgag caatcaactc
      541 ggcgcgttgt tggatgcgca ctggcccggg cccaaggccc tgttttaccg gctgggctcc
      601 gaaattgcgc tggccttcct caccaactac cccacccccg aatcggccgc ccgactcggt
      661 gagacccgca tggccgcctt cctgcgccgc cacgcctacc gcggcggcaa gacccccacc
      721 gaactgctga cccgccttcg cttggcaccc accccgccca ccgggctccc acccgaggta
      781 ctgacccaac tcatcggcgc ccaaatccag ctgctgcgca ccctgctcag caccatcacc
      841 gcactggaag cagagatcaa acgcatcgtc gccacccacc cccgggctcg ccttcttcag
      901 cagctacccg ggatcggcca gatcaacctg gcccagatcc tcgccgagat cggccccatc
      961 ctcgaccgcg tcgccgacgc cgaacaagcc gccgccgaat gcggcgccac acccgtcacc
     1021 aaagcctccg gcaagaccac tggcgtctac ttccgctggg ccgccaatac ccgcgcccgc
     1081 aaggcaatca ccgccttcgc ccacaactcc cggatgcaat cacgatgggc ccaaggcctc
     1141 taccgcgacg cccgcaaccg cggcaaacgc aacccccacg ccaaccgcat cgtcgcccgc
     1201 gcctggattc gcgtcatctg ggcctgctgg cacaccaaca ctccctacga cccccacaaa
     1261 cacaccgccg aacaacgcgc tgcagccacc ctgacttgac tcagaggact caagcgatac
     1321 atcgcccgcg aggtctaccg aaaactcatc aacaccccgc accacaccaa agacaagccc
     1381 gcagcggctt gacgaaccat agcgcctctg tatgtcaagt atctgccggc gagtcttgtt
     1441 gatctttggt gatgcgccgg tagaccgcgt cgctgagacg acgtttcagc gcgcgtcgcg
     1501 cttcggcggg agtcttcatc tcggc
//
LOCUS       DATADN010000544         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1128610, whole genome shotgun sequence.
ACCESSION   DATADN010000544 DATADN010000000
VERSION     DATADN010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128610"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1507
                     /locus_tag="VHH53_07065"
     CDS             <1..>1507
                     /locus_tag="VHH53_07065"
                     /inference="COORDINATES: protein motif:HMM:NF021588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HEX2289944.1"
                     /translation="AQTDQTTGDVVTDTPERPRRHPLLVWAWGLIRLDFAGVAVSALF
                     FCLSLTPSLLPRDWLFQGLIGGVNAAIGYGIGVFVSKMLARFVLRGRRRPRPSQRTLY
                     ALKAGIVGLAIAACVLMLVPAAAWQRQVSAEMGLPGPETSGYLRVLVVSALVGGVCVA
                     VSRMLLDIIKTLARWLIRRWRLSDEMALLIGTAVIVVLLITLINGVLMQGFLAGASRI
                     FQPQNTTTREGVSQPTQPERSGSPTSFAAWDSLGYQGRNFVASGPDAQELERVNGRPA
                     QEPIRVYAGLQTAETDEQRIAVLLSELERTGAFDRELLVIVPTTGTGWVNPVAARAIE
                     LMYNGDTAMVAMQYSYLPSWISFLGDQQRSMEAGRMLIDAVQQRWSQRAPQQRPELVL
                     YGESLGSMAGQAAFDWLPDIADMGFSSVLWVGPPNASPLWKAITQRRDPGTPEVQPRY
                     DGGRTVRFSQATDPDEIARDTQAPWEGTRVLFLQHASDPIVWYSPDLLTARP"
BASE COUNT          227 a          538 c          502 g          240 t
ORIGIN      
        1 gtgcgcagac cgaccagacc accggtgacg tcgtcaccga cacccccgaa cgcccgcggc
       61 gccatcccct gctcgtctgg gcatggggcc tgatccggct cgacttcgcc ggcgtcgccg
      121 tcagcgcgct gttcttctgc ctgtccttga ccccgtcgct gctgccgcgc gactggctgt
      181 tccagggcct catcggcggg gtgaacgccg ccatcggata cggcatcggc gtcttcgtct
      241 cgaagatgtt ggcgcggttc gtcttacgcg gcagacgccg gccccggccg tcgcagcgga
      301 cgctgtacgc gctcaaggcg ggcatcgtcg ggttggcgat cgccgcctgc gtgctgatgc
      361 tggtgcccgc cgcggcctgg cagcggcagg tctcggccga gatgggcctg cccggacccg
      421 agacatccgg ctacctgcgt gtgttggttg tctcagcgct cgtcggcggg gtgtgtgtcg
      481 cggtcagccg catgctgctc gacatcatca agacactggc ccgctggctc atccgccggt
      541 ggcgcctgag cgacgagatg gcgctgctga tcggcaccgc cgtgatcgtc gtcctgctca
      601 tcaccttgat caacggcgtc ctgatgcagg ggttcctggc cggcgccagc cgcatcttcc
      661 agccgcagaa caccaccacc cgcgagggcg tcagccagcc gacccaaccc gaacggtccg
      721 gcagcccgac atcgttcgcg gcatgggaca gcctcgggta ccagggacgc aacttcgtcg
      781 ccagcggacc cgacgcacag gaactcgagc gggtgaacgg caggcctgca caggaaccga
      841 tccgtgtcta cgccggcctg cagaccgccg aaaccgacga acagcgaatc gccgtgctgc
      901 tcagcgaact cgagcggacc ggggccttcg accgtgaact gctggtgatt gtgcccacca
      961 cggggaccgg ctgggtcaac cccgtcgccg cccgcgccat cgagctgatg tacaacggcg
     1021 acaccgccat ggtggcgatg cagtattcgt atctgccgag ctggatctcg ttcctcggcg
     1081 atcagcagcg gtcgatggag gccggtcgca tgctgatcga cgcggtgcag cagcgatggt
     1141 cacaacgggc accgcagcag cggccggaac tcgtgctcta cggcgagagt ctggggtcga
     1201 tggccgggca ggccgcgttc gactggttgc ccgacatcgc cgacatgggg ttctcctcgg
     1261 tgctgtgggt cgggccgccc aacgccagcc cgctgtggaa ggcgatcacg cagcgccgcg
     1321 accccggaac cccagaggtc cagccgcgct acgacggggg ccgcaccgtg cggttctccc
     1381 aggcgaccga tccggacgag atcgcccgcg acacacaggc tccgtgggag ggcacccgag
     1441 tgctgttcct gcagcacgcg tccgacccga tcgtctggta ctcaccggat ctgctcaccg
     1501 cccgccc
//
LOCUS       DATADN010000545         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_522224, whole genome shotgun sequence.
ACCESSION   DATADN010000545 DATADN010000000
VERSION     DATADN010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522224"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VHH53_07070"
     CDS             <1..822
                     /locus_tag="VHH53_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2289945.1"
                     /translation="AFSMSEPELGSDVAAIKTRARRDGDHYVVDGAKMWLTNGATSTL
                     VATLVKTDEGAAKPHHTLTTLLVDKPAGFGEVAPGLSVPGKIDKMGYKGVDTTELVFD
                     GHRVSAGKVLGDAPGKGFAQMMDGVEVGRVNVAARACGIAIRAFELAVGYAQQRRTFG
                     KPIAQHQAIAFKLAEMATKVEAAHLMMFNSARLKDSGARNDVQTGMAKLLASEYCKEG
                     TEDAFRIHGGYGYSKEFEIERLMREAPFLLIGEGTSEIQKMIISKGLLQEYAARS"
     gene            921..1406
                     /locus_tag="VHH53_07075"
     CDS             921..1406
                     /locus_tag="VHH53_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEX2289946.1"
                     /translation="MRGLPTPPTGWPIGSYATYAEAQRAVDHLADNHFPVAEVTIVGV
                     DLMLVERVLRRLTWGRVLTQGAASGAWFGLFVGVLLSLFATQAGAGVIPILVGVATGA
                     VFGTVFAAAGYASTRGRRDFASASQLVAGRYDVLCQPRHAEQARDLLAKLAMGPGTVT
                     T"
     gene            complement(1425..>1658)
                     /locus_tag="VHH53_07080"
     CDS             complement(1425..>1658)
                     /locus_tag="VHH53_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2289947.1"
                     /translation="ETGLDVKVARRAGAVQRCAPDGAVFDIHDYVCLATGGTLRAGDD
                     AADARWCDAETLARLPIVDGLVEALTEWDCLPL"
BASE COUNT          290 a          551 c          549 g          268 t
ORIGIN      
        1 gccttttcca tgtccgagcc agaactgggt tccgacgttg ccgcgatcaa gacccgggcc
       61 agacgcgacg gcgaccacta cgtcgtcgac ggcgcgaaga tgtggcttac caatggcgcg
      121 acgtcgactc tggtggcgac cctggtcaag accgacgagg gcgcggcgaa gccgcaccac
      181 accctcacca cgctgctggt ggacaagccg gcgggcttcg gtgaggtcgc ccccgggctc
      241 agtgtacccg gcaagatcga caagatgggc tacaagggag tcgacaccac cgagctggtc
      301 ttcgacggcc atcgcgtcag tgctggcaag gtgctcggcg acgcacccgg caagggcttc
      361 gcacagatga tggacggcgt cgaggtgggg cgggtcaacg tcgcagcccg agcctgcggc
      421 atcgccattc gcgccttcga gcttgcggtc ggctacgccc agcagcggcg gaccttcggc
      481 aagccgatcg cccagcacca ggcgatcgcg ttcaagctcg ccgagatggc caccaaggtc
      541 gaggccgcgc acctgatgat gttcaactcc gcccggctca aagactccgg tgctcgcaat
      601 gacgtccaga ccgggatggc gaagctgctg gccagcgaat actgcaagga gggcaccgag
      661 gacgcctttc gtatccacgg cggatacggc tactccaagg agttcgagat cgagcggctg
      721 atgcgggagg cgccgttcct gctcatcggt gagggaacca gcgagatcca gaagatgatc
      781 attagtaagg gactgctgca ggagtacgct gcgcggagtt gatccgctaa tcggggccgt
      841 gcgcatgagc cttggcacga tgggtacccc gcagccagag gtgatgcact gtgactagcc
      901 ccttcagcaa ccagggccag atgcgtggcc tgcccacccc acccaccggt tggccgatcg
      961 gctcctatgc cacttacgcc gaggcacagc gtgccgtgga tcatctcgct gacaatcact
     1021 ttccggtcgc cgaagtaacc atcgtcggag tggacctgat gctcgtcgag cgggtgttgc
     1081 gacggttgac ctggggccgg gtgctgactc agggggccgc gtcgggagcc tggttcggcc
     1141 tgttcgtcgg agtgctgctc agcctgttcg ccacccaagc cggggccggg gtcatcccga
     1201 tcctggtggg cgtggccacc ggtgcggtct tcgggacggt gttcgcagcc gccggttacg
     1261 cctcgactcg tggccggcgc gacttcgcgt cggccagcca gctcgtcgca gggcgttatg
     1321 acgtgctgtg ccaaccccgt cacgccgaac aggcccgcga cctgctcgcc aagctggcca
     1381 tgggccccgg gacggtcacc acctgagctc agcggtcaca gtgatcacag gggcaggcaa
     1441 tcccactcgg tgagggcttc aaccaggccg tccacgatcg gtagccgcgc cagggtctcc
     1501 gcgtcgcacc accgggcatc ggcagcatcg tcgccagctc gcagcgtccc ccctgttgcc
     1561 aggcagacgt agtcatggat atcgaagact gcgccatcag gcgcgcagcg ctgaaccgct
     1621 ccggcccgac gggcaacctt gacgtccaac ccggtctc
//
LOCUS       DATADN010000546         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1128668, whole genome shotgun sequence.
ACCESSION   DATADN010000546 DATADN010000000
VERSION     DATADN010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128668"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VHH53_07085"
     CDS             complement(<1..1137)
                     /locus_tag="VHH53_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289948.1"
                     /translation="MTPRVLRGRTAAALLIVVIALFGGQAALGAPALAQVPGVEDCKD
                     APNPERPGSGMVGAIDPTPLQAGAPDSVYHEVGYAGLVWHTYDLGCGPSGLRNPNAVI
                     DTWTGGLLFDIGKAVVGASNGLHYALADGGLLAPLNDLIVSGTVALYDTVYAPWFGLI
                     AVLLAIVLFRSIWRGDLATVSKRAAWALAGIWFAAATYLTPLLYTQVLDGVLVEGSNQ
                     IRTGFLDEVGIEQRDALPTLLHDQIIYRNWLRGEFGSPDGPQAVQLGRDLVRAQAFTK
                     QEIADGADNPESGVAERKKEEYKAIAARTGSAYGHFQGVDGNRIGVGFLAAVQSLMFG
                     LFQFLAQAALLLAQVLLRVVILLGPVIGLIAVLYHELLRGVGRVV"
     gene            complement(1134..>1869)
                     /locus_tag="VHH53_07090"
     CDS             complement(1134..>1869)
                     /locus_tag="VHH53_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2289949.1"
                     /translation="TEVRDDRLTVLTMSGLTLPKDTIGREHWTDAESLGVELLNLAAW
                     LTQRSIYERPRDQRKGVWIDEAFFLSEVPTGRVLMNRFARDSRKWNVRVLLSSQIPAD
                     FLKIQGFVSLLDSVFVGRLDDGPAQSDALRLLKVPVEAGYEQVVASLGRRPGGERLAT
                     ERDRAPRQFIFGDGAGGVERVRVDFSGAHLEHLCAALDTTPDALRGDVSVGELPGGDF
                     RPAIDPERSGPADGEIGREVTTRAQA"
BASE COUNT          325 a          658 c          618 g          268 t
ORIGIN      
        1 gacgacgcgc ccgacgccac gcagcagttc gtggtacagg accgcgatca ggccgatcac
       61 cggcccgagc aggatgacca ctcggagcag gacctgggcc agcagcaagg ctgcctgggc
      121 gagaaactgg aacaggccga acatcagtga ctgcacagcg gccaggaaac cgacgccgat
      181 ccggttgcca tccacgcctt ggaagtggcc gtaggcgctg ccggtgcggg cggcgattgc
      241 cttgtactcc tccttcttgc gctcggcgac gcccgattcc ggattgtccg cgccgtcggc
      301 gatctcctgc ttggtgaagg cctgcgcgcg gaccaggtcg cggcccagct ggacggcctg
      361 cggcccgtcc ggggaaccga actccccacg cagccaattg cggtagatga tctggtcgtg
      421 cagcagggtg ggcagcgcat cgcgctgctc gataccgacc tcgtccagga acccggtccg
      481 gatctggttg ctgccctcga ccagcacccc atcgagcacc tgggtgtaca gcaacggagt
      541 gaggtaggtc gctgcggcga accagatgcc ggccagcgcc cacgctgccc gcttgctgac
      601 cgtggccagg tcaccccgcc agatcgaccg gaacaacacg atcgccaaca gcaccgcgat
      661 caatccgaac cagggtgcgt acacggtgtc gtagagcgcg acagtgcctg agacgatcag
      721 gtcgttcaac ggggccagca gccctccgtc ggcaagcgcg tagtgcagcc cgttgctggc
      781 gcccaccacc gccttgccga tgtcgaacag cagcccgcca gtccaggtgt caatcaccgc
      841 gttcggattg cgcaggcctg atgggccaca accaaggtcg taggtgtgcc acaccagccc
      901 ggcgtaaccg acctcgtgat agacgctgtc cggcgcgccg gcttgcaacg gcgtggggtc
      961 gatggcgccg accatgccgg agccgggtcg ctccgggttg ggcgcgtcct tgcagtcctc
     1021 aacgccagga acctgcgcca gagctggtgc gcccagcgct gcctgcccgc cgaacaacgc
     1081 gatgaccacg atcagcagcg ctgcagccgt ccggccccgc agcacccggg gcgtcatgct
     1141 tgggccctgg tggtgacctc gcgaccgatc tcgccgtcgg ctgggccgct gcgctcggga
     1201 tcgatcgcag gcctaaagtc tccaccgggc aactcaccga ccgagacgtc accgcgcagg
     1261 gcatccggag tggtgtccag cgcggcgcac aggtgctcca ggtgggcacc ggagaagtcc
     1321 acccggaccc gctccactcc gccggcgccg tcgccgaaga tgaactgccg gggtgcccgg
     1381 tcccgttcgg tggccagccg ttcaccgccc gggcggcggc cgagggaggc aaccacctgc
     1441 tcgtagccag cctcgacagg caccttgagc agtcgcagcg cgtcggactg cgccggcccg
     1501 tcgtccagcc tccccacgaa cacggagtcc agcagcgaca cgaaaccctg gatcttgagg
     1561 aaatcggctg gaatctgcga cgacagcagc acccgcacgt tccacttgcg ggaatcacgg
     1621 gcgaaacggt tcatcagcac acggccggtg ggcacctcgg acaggaagaa cgcctcgtcg
     1681 atccagaccc ccttgcgctg gtcgcggggg cgctcgtaga tcgaccgctg ggtcagccag
     1741 gcggccaggt tgagcagctc cacaccgagg gactcggcat cggtccagtg ctccctgccg
     1801 atggtgtcct tgggcagggt cagcccggac atggtgagca cggtgagccg gtcgtcacgc
     1861 acctcggtg
//
LOCUS       DATADN010000547         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1129164, whole genome shotgun sequence.
ACCESSION   DATADN010000547 DATADN010000000
VERSION     DATADN010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129164"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1315)
                     /locus_tag="VHH53_07095"
                     /pseudo
     CDS             complement(174..1315)
                     /locus_tag="VHH53_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1498..1570)
                     /locus_tag="VHH53_07100"
     tRNA            complement(1498..1570)
                     /locus_tag="VHH53_07100"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1535..1537),aa:Ile,seq:cat)
BASE COUNT          292 a          548 c          552 g          250 t
ORIGIN      
        1 gtcaagcatc agccgttacc gcgtcaagca tcagccgata ccgcggaaaa cgtcagccgt
       61 taccaatccg acaaggatca ggcgtgctca aacaattacg cagtacgcct gaagtgttcc
      121 ggtagacggt tgactaaacg gttccggttg atgctttaca catcgaccgg gtgtcagtag
      181 cggcgggcgt tcttcttgcg gacctcccct cggctggttc gcaggacagt acggactcgg
      241 gcgccggtgc accagacgtg gagcaggtcg tcgtcgacgt ggacggtgac cagctttccg
      301 gcgagcgcgg cgccgacgga gaagacctgg ccggagacgg agatgatccc gttgctggcc
      361 actttgcggg tgacggtggc gaggggatcg gccggtggtt cctggaggac gacctcggac
      421 acctggccgc ggagggcgaa ccgttcggcg ggggtgagca tgccgatggc ctggtggggc
      481 cgggccttgt tgtactcggc cacccagtcg tccagggcgg cctgggcggc ggccaggccg
      541 gtcagggccc ggtcggccag gaactcagcc cgcagggtgc ggtggaagcg ctcgatcttg
      601 cccgtggtgg tgggggaacg gacgccggtg aggcggtggg tgatgccgtt ctggcggcag
      661 atccggtcga agagcacctc ggaggggatg gaggagtggc ggccggtgaa gaccttgccg
      721 ttgtcggtca ggacctcgtc gggcacgccg tgttcccgca tggcgtccac gaagtggccg
      781 cacaccgccc gggaccgggc gtgttccatc agcccggcgc agacacagaa gcgggagtgg
      841 tcatcgatcc cggtcagggc cttgagctcg gtcccgtcga ccaggttgat cccgccgacc
      901 acgtcgagtt gccacagctc catcggccgg tgccgttccc agcggaccca gtccttccgg
      961 cgccgctgat gggccggttc gatcatccgc tgacggatca acgcccgata gaccgccgaa
     1021 cgcgacggcg ccggctccac gccccgtttg gccagcaccc aggccagccg cgtcgggccc
     1081 caccccggat gctggcggcg cagctcacac aactcggcct ccaccgccgc aggcatctga
     1141 tggggacagc cgaacggctg atgcgaccgg tcctccagcg ccgtcagcca ccctgctcat
     1201 aaccgcggat ccagctgtgg accgacgggc gggacacccc aaaccgggag gccacctccg
     1261 tcaccggctg cccgtcccgg accaccgcca tcaccgcttc gaaacgctgt tccatgacgc
     1321 tgcgctcgat gagcacccgc cccaccccct cgcccagaaa cccccagcga gaggagaaga
     1381 ccgccgcccg actgccaagg atcagccgga actcgcgtca agcatcagcc gaaactgtgt
     1441 aaagcatcag ccgaacccga agtgacaagg atcagccgag acaacacacg ggagcggtgg
     1501 gcccggcagg gatcgaacct gcgaccaaga gattatgagt cccctgctct aaccactgag
     1561 ctacgggccc ctggtgaatc ggctcctgac ctgcgctttc ctgtcgcatc gcgtgtgcta
     1621 gggtgcgagc atagcgctga gc
//
LOCUS       DATADN010000548         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_979012, whole genome shotgun sequence.
ACCESSION   DATADN010000548 DATADN010000000
VERSION     DATADN010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979012"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VHH53_07105"
     CDS             <1..963
                     /locus_tag="VHH53_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2289950.1"
                     /translation="ASDEPLFVEPSQRHGLHGRMVVAVRAGRELLGSLWVESDTPLTS
                     HRRTVLEDGARTAALHLLRLRASADLERQVESDLVIRLLEGTAETMAMISQLGLPPGN
                     FRVIALQAHVGNERHAGVLLAFERATTGFGWSRHGRSALFGNTVYTMWPCRDDDLTRA
                     RNWIDTVVAELPGHVTVSAGIGGPAAAAALPASRQEADESLALHAARPGTAAVAYDES
                     WDEILLQRLRVAASLGRAPTRGPIVELRRHDAEHATRYIETLRAWLQAQGDMGDAAVR
                     LEVHPNTVRYRLRKMAEATNLELDRPDKRLAMIIALAATDHQTP"
BASE COUNT          273 a          527 c          538 g          240 t
ORIGIN      
        1 gcgtcggatg aaccgttgtt cgtggagccg tcgcaacgcc atgggttgca tggccgcatg
       61 gtggtcgcgg tacgcgccgg gcgagagctg ctcgggtcgc tttgggtgga atcggacaca
      121 ccgttgacca gccatcgccg gaccgttctc gaagacggtg cgcgcaccgc tgcgctgcat
      181 ctgttgcggt tgcgggccag cgcagacctg gagcgccagg tcgagtccga tctggtgatc
      241 cggttgctgg agggcacggc ggagacgatg gccatgatca gtcagctcgg cctgccgcca
      301 ggcaacttcc gggtcatcgc gctacaggcc catgtgggca acgagagaca tgccggagtc
      361 ctgttggcct tcgaacgagc gaccaccggt ttcggctggt cgcggcatgg tcgcagcgcg
      421 ctgttcggca acaccgtcta cacgatgtgg ccgtgtaggg acgacgacct gacgcgagcg
      481 cgcaactgga tcgacaccgt ggtcgccgag ttgcccggac acgtgaccgt gtccgccggt
      541 atcggcgggc ccgcggctgc ggcggcgctg cccgcgagtc gtcaagaagc cgatgagagc
      601 ttggcgctgc atgccgcccg accaggcacg gctgccgtgg cctatgacga atcatgggac
      661 gagatcttgc tgcagcgcct acgggtggcc gcatcgttgg gacgcgcgcc aacccgcggg
      721 cccatcgtcg agctgcgccg acacgacgcc gagcacgcca cccgctacat cgagacgctt
      781 cgggcctggc tgcaggctca aggagacatg ggagacgcgg cagtccgttt ggaggtccat
      841 ccgaacaccg tccggtatcg actgcgcaag atggccgagg ccaccaatct cgagctggac
      901 cggcccgaca agcgcctagc aatgatcatc gcgctcgcgg ccaccgacca ccagacgccc
      961 tgaccccaca gccctcttgt tgcgaggcac agatcccggc cacccggttg tcgattctgc
     1021 acagacccct ccgggtcgag cggggccatc ctgaagtaca gcgaggcaga ccgtgggctg
     1081 ggcggtggac ggtccagcgc caggctggag accactcagg aggtgtgtcg tggtcagtga
     1141 tcgcgagcgg agagcgctgt cggaggccca aagcccagtc cagatcaagg attcggaccg
     1201 cgcccatctc ctcgaggccg tcgagcctca ccgaccgcgc gatcaccacc ggtgggcctt
     1261 cccggtcggg gtcgtcctcg ccgtagtgct caccgtgccc ggcccgagca ccctgataga
     1321 agccgagatt gccgcccgaa agaggccgcc aaggccgcga acccggcgta atgccgtcac
     1381 ctcgccgata gggagaccct cgctgggatc gcgggtacgg ccaccgtcgg ctgcatgacg
     1441 cacccggtga gcggggtcga acgcgtttca acgcgccgca ctcggcagct ggatcaagac
     1501 ctgatggccc cccgaacctg aggagcacgg aggaagcctg atgacatcgc ccgactcgtc
     1561 gcacctggtg caggatcg
//
LOCUS       DATADN010000549         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_207423, whole genome shotgun sequence.
ACCESSION   DATADN010000549 DATADN010000000
VERSION     DATADN010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207423"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..>2004
                     /locus_tag="VHH53_07110"
     CDS             149..>2004
                     /locus_tag="VHH53_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2289951.1"
                     /translation="MGLRSIDGHLVRNGQEVYAWYRLAPQRWSFRSDSQRQDLIHAIA
                     GQYAELQGRWLHLRVTARPYPIRMWAEAHARNAVGRLPDTPGALSFDDYMVGEQQQLL
                     GHSMAEKEVYLGVQVQTRSMVDRVVERAAPVLRRVFPTAVDAELVAVDAEVEHLDGVI
                     GVAGLEGRPVTAEEMSWLMHRSCSLGLPAPRNLPVVPGASWEPEDLASFTDAADFHTE
                     PYAPTLTVRGRTGSNAGIARHVVVLTVGQMHGLRIPEIDDPWVQRSDRLPAGVEWSAR
                     IYVRRPEEVAGELQRQMNKVRSQVRHYTDEHDLEPPQSLVRQAGRVLEIDDEMTSGFT
                     ALGTRVRSWWRLAVSGPTDRDALRLAQQLLDLYKPKIAIEHPEAQYALAREFIPGEPL
                     SSGAYLRRGSVIWAASAVPTATAEVGDRRGILLGETCTATRRPVAWDPWMAQEIRDSS
                     GLTALVAGLGGGKSFLGGGIVYKTLRSGAYWTLLDPSGPLAKLCELPELRPYARPINL
                     LNAQPGILNPYRVVAEPELAHFLDEEDPERSWRREKALTAATRRRLVLDVLTGVLPFE
                     VARMAKTRIVLLRAVRAVGGRPDAHPGQVFEALRRDPSEHHEHAIVVADFLD"
BASE COUNT          282 a          712 c          701 g          309 t
ORIGIN      
        1 tgccagctgg gagcccgagg acctggccag tttcaccgac gccgcggact tccacaccga
       61 gccctacgcg cccacccctc gaggaggctc gaaccggcgc agccacaaac tccccggaga
      121 agccgccgcc cccacctaca cgcccctcat cggcttacgt tccatcgatg gccacctggt
      181 acgcaacggg caagaggtct atgcctggta ccggctcgcc ccgcagcgct ggtcgtttcg
      241 ctcggactcg caacggcagg acctgatcca cgccatcgcc ggccagtatg cggagttgca
      301 gggccggtgg ctgcacctgc gggtcaccgc ccgcccgtac ccgatccgga tgtgggccga
      361 ggcgcacgcg cgtaatgcgg tgggccggct acctgacacc cccggggcgc tgtccttcga
      421 cgactacatg gtcggtgagc agcagcaact gctcggccat tcgatggctg agaaggaggt
      481 ctatctcggc gtgcaggtgc agacccggtc catggttgat cgggtcgtcg agcgggccgc
      541 accggtgctg cggcgggtct ttcccaccgc agtcgacgcc gaactggtcg cggtggacgc
      601 cgaggtcgag cacctcgacg gggtgatcgg cgtcgccggg ttggagggca ggccggtcac
      661 cgctgaggag atgtcctggc tgatgcatcg gtcgtgttcg ctgggactgc cagccccacg
      721 caacctgccc gtcgtgcccg gtgccagctg ggagcccgag gacctggcca gtttcaccga
      781 cgccgcggac ttccacaccg agccctacgc gcccaccctc accgtccgcg gtcgaaccgg
      841 ctccaacgcc gggatcgctc ggcacgtcgt ggtgctcacc gtgggacaga tgcacgggtt
      901 gcggatcccg gagatcgacg atccctgggt gcagcgctcg gaccggttgc cggctggggt
      961 cgaatggtcg gcacgcatct acgtgcgccg cccggaggag gtggccggcg agctgcagcg
     1021 gcagatgaac aaggtgcgct cccaggtgcg gcactacacc gacgagcacg acctcgagcc
     1081 gccgcagagc ctggtccgcc aggccggccg ggtgctggag atcgacgacg agatgacgtc
     1141 gggtttcacc gcgctgggta cccgggtgcg gtcgtggtgg cggctggcgg tctccgggcc
     1201 caccgatcgg gatgcgctgc ggctggccca gcagctactg gatctctaca agcccaagat
     1261 cgccatcgag catcccgagg cgcagtacgc attggcccgc gagttcatcc ccggcgagcc
     1321 gctgtcctcg ggcgcctacc tgcgccgcgg ttcggtgatc tgggctgcct cggcggtgcc
     1381 caccgccacc gcggaggtgg gggaccggcg cggcatcctg ctcggcgaga cgtgcaccgc
     1441 cacccggcgc ccggtcgcct gggatccctg gatggcccag gagatccggg actcgtccgg
     1501 gttgaccgcg ctggtggccg ggctcggcgg cggcaagtcg ttcctcgggg gcggcatcgt
     1561 ctacaagacg ttgcgttccg gtgcgtactg gacgctgctg gatccctccg ggccgctggc
     1621 caagctgtgt gagctgcccg agctgcgccc ctacgcccgg ccgatcaacc tgctcaacgc
     1681 ccagcccggc atcctcaacc cgtatcgggt ggtcgccgag cccgagctgg cgcatttcct
     1741 cgatgaggag gacccggagc gctcctggcg gcgggagaag gcgctgactg ctgccacccg
     1801 gcgccggttg gtgctcgacg tgctcaccgg tgtgctgccc ttcgaggtcg cccggatggc
     1861 caagacccgc atcgtgctgc tccgcgcggt ccgggcggtg ggcgggcgcc ccgatgcgca
     1921 ccccgggcag gtgttcgagg cgttgcgccg cgatccgtcc gagcaccacg agcacgccat
     1981 cgtggtcgcc gacttcctcg acga
//
LOCUS       DATADN010000550         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_979227, whole genome shotgun sequence.
ACCESSION   DATADN010000550 DATADN010000000
VERSION     DATADN010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979227"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VHH53_07115"
     CDS             <1..334
                     /locus_tag="VHH53_07115"
                     /inference="COORDINATES: protein motif:HMM:NF041510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMED_5909 family protein"
                     /protein_id="HEX2289952.1"
                     /translation="PRAGPGRDPGAATAKLTAPVASAVTTLRDAHDLIRRLRPASDAP
                     VAEWLAFRRRAAQIYTTVADADRYHHHEALYWVQREREAADELAAQIRASGREPDLPS
                     RPAPGRVQ"
     gene            757..>1690
                     /locus_tag="VHH53_07120"
     CDS             757..>1690
                     /locus_tag="VHH53_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEX2289953.1"
                     /translation="MDAYLIAMVIAGGIALGVVVWVLAKVGKALIQMAEALAAAAVVV
                     LALWLTIKALVWAIRQAVTHWRTSLTVLAVTACWHWAGWLALALTVGVVAGVLTGWRL
                     LDLISFHAWVGRYLRAWWLRWTVYAPKLPQWLHACGLSIKPDAMPVIVTITPLRRTLR
                     RTRQHTRAQLPKVLGVRSGASWDEVRIRLVPGQKPEDFDQAARALAAARGVTRCQVRE
                     LTPNVVSIDFQRRNLLADPVACPNLTTLANVGGASIDLGRVWAGRTEYGQDWHVPLAG
                     SASHTLTAGASGAGKNSVMWCPLVSIAPAIRDGLV"
BASE COUNT          297 a          560 c          563 g          270 t
ORIGIN      
        1 cccgcgcgcg ggacccgggc gggaccccgg ggcagcgacc gcgaagctga cggcgccggt
       61 ggcctccgcg gtcacgacgc tgcgagacgc gcatgacctg atacgccgac tccggcccgc
      121 ctccgatgct ccggtggccg agtggctggc atttcggcgg cgcgcggcac agatctacac
      181 cacggtggcc gatgccgatc gataccacca tcacgaggcg ctgtattggg tgcagcgcga
      241 acgcgaggca gctgacgagt tggccgccca gatccgggcc agcggcaggg agccagatct
      301 cccgagtagg ccggcgccgg gacgggtgca gtagctcgcg gcctgccgct cttagcacct
      361 ctacaactct caggcggaaa tgcggtgatg caccgaaaac gatagaccaa taattctagc
      421 gatagtaaac agaccacggg catgggtcgc gtggcgataa agcctgtgcc aatttcagga
      481 taacgagaat aaggcgcaga agcggattcc tggctaccaa cctgttccgc ttctgcgctg
      541 tccacccagt caggagtgaa cagtagtgag aatatgcagg gctaccgaga ccggtcaact
      601 tgacaaccgc aatgaacaga cctgcccgca ccggcaacag ataggcacct ctgtccccgg
      661 tcagggttcc agtgacaagg gcactggggt actgccgtcg ctgttcacgg ggccgatcag
      721 agtgatcacc ggtctcgcgg tcgtgggtga tcgctgatgg atgcctacct gatcgcgatg
      781 gtgatcgcgg gtggcatcgc gctgggcgtg gtggtgtggg tgctggccaa ggtcggcaag
      841 gcactgattc agatggccga ggcgctggcc gcagcagcag tcgtcgtcct ggccctgtgg
      901 ctgacgatca aggcgctggt gtgggcgatc cgccaagcgg tcacccactg gcggaccagc
      961 ctgaccgtgc tggcggttac ggcatgctgg cactgggcag gctggctagc gctggccctc
     1021 acggtcggcg tggtcgcggg agtgctcacc gggtggcggc tgctcgatct catctccttc
     1081 catgcctggg tgggtcggta tctgcgggcc tggtggctgc gctggacggt gtacgccccc
     1141 aagctgcccc agtggctgca cgcctgcggc ctgagcatca aacccgacgc catgcccgtc
     1201 atcgtgacaa tcacccccct tcgccgcact ctccggcgga cacgacagca cacccgggcg
     1261 cagctaccca aggtgctcgg cgtgcgctcg ggcgcatcct gggacgaagt ccggatccga
     1321 ctggtgcccg gccaaaaacc ggaagacttc gaccaggccg cacgggcgtt ggccgctgcc
     1381 cgcggagtga cccgctgcca ggtccgcgaa ctgaccccca acgtggtgtc aatcgatttc
     1441 cagcgccgca acctacttgc cgacccggtg gcctgcccga atctcaccac cctggccaac
     1501 gtcgggggcg caagcatcga tctaggacgg gtgtgggccg ggcgcaccga atacggccaa
     1561 gactggcacg tgcccctggc cggctcagcc agtcacaccc tgaccgccgg cgccagcgga
     1621 gccggcaaga actcggtgat gtggtgccca ctggtctcca tcgctcccgc catccgcgac
     1681 ggcctggtac
//
LOCUS       DATADN010000551         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_979340, whole genome shotgun sequence.
ACCESSION   DATADN010000551 DATADN010000000
VERSION     DATADN010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979340"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..621
                     /locus_tag="VHH53_07125"
     CDS             61..621
                     /locus_tag="VHH53_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005152006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2289954.1"
                     /translation="MRTLAITQNITVDGSIEMLGDWFDPQGQAGVDNSDLLEELHRQD
                     SRADGFLVGRQTFEDLRGYWPKQADDATGITDYLNRVQKYVVSSTMTDPEWQNSTVLS
                     GDPVEEVRALKEQSGQDIVITGSIRLCHTLIRAGLVDQYRLFVYPVVQGRGRRLFPDG
                     FELPKLTLLEAKAFRSGITYSRYARS"
     gene            994..2142
                     /locus_tag="VHH53_07130"
     CDS             994..2142
                     /locus_tag="VHH53_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2289955.1"
                     /translation="MRILFGSWPGYGHLLPMVPLIRAAQQGGHDVVVSSGADMSALLG
                     RLGVAAHRSGVTLAESYQRMPGQATISELPAEQQPGFAARHLFGAGAVDRAHDLLDLV
                     QRWRPDLVVHDTLELGSPTAAALHGIPHVTHGYGPTVPNTRYFAAAIAAAISEAGLPD
                     PTPDIFAAPYLDICPPSLRGTEPSPWTTIHPLRPSPGETEPHAAVALDLSALPHPDTV
                     YLTLGTIMNQTPKVFRAVIDGCMRWPVNLVVTTGPGFEPALLGPLPPAVRTAPYLPQA
                     AVLPHCRAVVSHAGAGTMLGALYYGLPQLCLPQGTDQPSNTAALLPTGAALALHPDEI
                     TADVVAEALGRLLNEPSFRLAANRLRAEIERMPTAAAVLHELLVTTDQ"
     gene            complement(2658..3194)
                     /locus_tag="VHH53_07135"
     CDS             complement(2658..3194)
                     /locus_tag="VHH53_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289956.1"
                     /translation="MVPAASMSTGTAPGVLVADVSFRPTSLEEGRFTRADLVFSGVDH
                     SETSYEVRLFLNNPTADASTPRTAEQGYAGRFVVFGHGGCFGDEGHCEVPSQPRASTD
                     LRLAHPLTPQTKLVTVTDALRRVLAEQEEGLRQVTLVPVSKDPSQERRGPTDSLFRFD
                     GLELQTYLTETEEVPTSA"
     gene            complement(3205..>3925)
                     /locus_tag="VHH53_07140"
     CDS             complement(3205..>3925)
                     /locus_tag="VHH53_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEX2289957.1"
                     /translation="AFLAVMGLRDERGYQYHAGLHGLPLPIQCRHGDILFLPWHRAYL
                     YFFELALQDRVPDGAVPWWDWTSATAHQDGLPPTHAPQAQEDGRPNALFDSEVLLPPA
                     ELDLVRERLPGALTEDDPPRTRRDPDLPDELPRDRTIRSILRAPTFSDFSFRLENVHN
                     DVHVWVGGSMSAVPVAGFDPVFWSHHAMIDRLWYLWQLAHPGALPPAQTLDIALAPFP
                     MTVRQTLDISALGYEYAVQTS"
BASE COUNT          659 a         1344 c         1298 g          624 t
ORIGIN      
        1 ggggcaagaa caaaagacca tcagcgacag cgagacctga gaaggaggag gacgccgaca
       61 ttgcggactc tcgccatcac ccaaaacatt accgtcgatg gctccatcga gatgctgggg
      121 gactggttcg acccgcaggg ccaggccggt gtggacaact cagatctgct tgaagagctg
      181 caccgacagg acagcagagc cgacgggttt ctcgtgggcc ggcagacctt tgaggatctg
      241 cgcggctact ggcccaagca agccgacgac gccaccggca tcactgacta cctcaaccgg
      301 gtgcagaagt acgtcgtgtc ctcgaccatg accgacccgg agtggcagaa ctccacggtc
      361 ctgtccggtg atcccgtcga ggaggtacga gcgctgaagg agcagtccgg ccaggacatc
      421 gtcatcaccg gcagcatcag gctgtgtcac accctcatca gggccgggct ggtcgaccag
      481 taccggttat tcgtctaccc cgtcgtgcag ggccgcggac gtcggctgtt ccccgacggg
      541 ttcgagttgc cgaagctgac gctgctcgaa gcgaaggcct tccgaagcgg catcacctat
      601 tcgcgctacg cccgctcctg acagggtcgc cgacgcgcga aggtgctggt caggttagta
      661 ttaggactcg taggcttcgg accccggtac acctcatccc taatcgagat cgacggtcaa
      721 gcgctgtgcc atgccgcatc cgcgcaggtg gcgcctatcc gtgagtgtca cagggatggg
      781 gtgaaccggc gggcctcctt gacgctccgc tgcttccagc cgaagggacg ccagacaaga
      841 aatggttgcc gccagcacct ccctgtggca ggcgccgccc tcgccgggcc cgatgggccg
      901 gggcctcggc ttcggactgt cctgtgactg cgcgtcacgg ccggcgggag agctgaccgc
      961 aaacgagttt cgtgggcctc aggagttgtg ccgatgagga tcctgtttgg ttcctggccg
     1021 gggtacggtc acctactgcc gatggtgccg ttgatccggg ctgcgcagca gggcggtcac
     1081 gacgtcgtag tctcctctgg cgccgacatg tcggccctgc ttggccggct cggcgtcgcc
     1141 gcccaccgtt ccggcgtcac gctggccgag tcctaccagc ggatgccggg ccaagcgacg
     1201 atcagcgagc tgcctgccga gcagcagccc ggcttcgcgg cccgtcacct gtttggggcc
     1261 ggcgccgtcg accgtgccca tgacctgctc gacctggtgc agcggtggcg gcccgacctc
     1321 gtcgtgcatg acacgttgga actggggtcg ccgaccgccg ctgcccttca tgggataccg
     1381 cacgtcaccc acggctacgg cccgacggtc ccgaataccc gctacttcgc agccgcgatc
     1441 gcagcggcca tctccgaggc aggcttgcct gatccgacac ctgacatctt cgccgcaccg
     1501 tacctcgaca tctgcccacc gagcctgcgc ggaaccgaac cgagtccgtg gaccaccatc
     1561 cacccgctgc ggccctcgcc tggcgagacc gagccgcacg ccgcggtcgc gctggacttg
     1621 tccgcactgc cccacccgga caccgtctat ctcaccctcg gcacgatcat gaaccagacc
     1681 ccgaaggtgt tccgggccgt gatcgacggc tgcatgcgct ggcccgtcaa cctcgtggtg
     1741 accaccggac ccggcttcga gcccgcgctg ctcggacccc tgccgccagc ggtgcggacc
     1801 gcgccgtacc ttccccaggc ggccgtgctg ccccactgtc gagcggtcgt gtcccacgcc
     1861 ggagcaggca ccatgctggg cgccctgtac tacggcctgc cccagctgtg cctgccacaa
     1921 ggcaccgacc agccgtccaa caccgcggcg ctgctgccca ccggcgccgc actggctctc
     1981 cacccggacg agatcaccgc cgacgtggtg gccgaggccc ttggccggct gctcaacgag
     2041 ccgtcgttcc ggttggcggc caaccggctc cgcgccgaga tcgagcgcat gccaaccgcc
     2101 gcggccgtcc tccacgaact cctggtcacc accgaccagt gaccgccatc cgcaggcaac
     2161 gccgccaggt atcgacattt ctggctgtca gatcccagac caccgggtcg cacaccatca
     2221 tcagcacctg ccgcgtgagc tgaatacttc gcgcgtggct ggggccgcaa ctccttggcg
     2281 aaggtgacgc caacaaggtg tccccttcga gcttggagtc caactgatag tccaacaccg
     2341 ggatgggttc tgacaccgct ggggagccga cgatctaggg catgtcaacg acggcgggcc
     2401 gcaaagaggc tgatcaaagg agttgttcgc ctcagaaagg gagcgcccgc aagacctcac
     2461 cccaaacggt acgcatcgga tcgggctggc accgcaagac ccacagacca gcgtttccgc
     2521 tggtcacgaa ccgatctgtg ggtctggctg gacttgaacc agcgccctcg tccttatcag
     2581 agatcgacgg ccgagcgccg tgcaacaatc cctgcgtggc acgcccccgc cgggtttgcg
     2641 tcggctcggg tcgggtgcta ggcggaggtg ggtacttcct ccgtctcggt caggtaggtc
     2701 tgcagctcga ggccgtcgaa gcggaacagg ctgtcggtgg gcccgcgccg ctcctggctt
     2761 gggtccttgg agacaggcac cagggtgacc tggcgcagtc cctcctcttg ttcggccagc
     2821 acccggcgga gcgcatcggt cacggtgacg agcttggtct ggggggtcag cggatgcgca
     2881 aggcgaaggt cggtgctggc ccggggctgg ctggggacct cacaatggcc ttcgtccccg
     2941 aagcagccgc cgtggccgaa gaccacgaac cgcccggcgt agccctgctc ggccgttcgg
     3001 ggcgtgctcg cgtccgcggt tgggttgttc aggaacaggc gtacctcgta ggaggtctcg
     3061 ctgtgatcga cccccgagaa gaccagatcg gcgcgggtga accggccttc ctcgaggctg
     3121 gtgggccgga aggagacgtc ggcgacgagc acgccgggcg ctgtgcccgt cgacattgat
     3181 gcggcaggga ccattgctgc cctcctagct ggtctgcaca gcgtactcgt agccgagagc
     3241 cgagatgtcc agcgtctggc ggaccgtcat gggaaacggg gccaacgcga tgtcgagcgt
     3301 ctgggcgggt ggcagggctc cgggatgggc cagctgccag aggtaccaca gccggtcgat
     3361 catggcgtga tgggaccaga agaccgggtc gaagccggcc acaggaaccg cggacatgct
     3421 gccgccgacc cacacatgga cgtcgttgtg gacattctcc aaccggaagc tgaagtcgct
     3481 gaaggtcggc gcccgtagga tcgaccggat tgtccggtcc cggggaagct catcgggcag
     3541 gtcggggtct cgacgggtgc ggggtggatc gtcctcggtc agcgctccgg gcagccgttc
     3601 cctgaccagg tccagctcgg ccggcggcag gagcacctcg ctgtcgaaca gggcgttggg
     3661 ccgcccgtcc tcttgcgcct ggggagcatg ggtgggcggc agcccatcct ggtgcgccgt
     3721 cgccgaggtc cagtcccacc acgggacggc cccgtcgggc acacggtcct gcaacgcgag
     3781 ctcgaagaag tacaggtagg cccgatgcca gggcagaaac aagatgtccc cgtgccggca
     3841 ctggatgggc aacggcaacc cgtggagccc ggcgtgatac tggtagccac gctcgtcacg
     3901 gagccccatc accgccagga aggcc
//
LOCUS       DATADN010000552         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_979536, whole genome shotgun sequence.
ACCESSION   DATADN010000552 DATADN010000000
VERSION     DATADN010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979536"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..>1734)
                     /locus_tag="VHH53_07145"
     CDS             complement(273..>1734)
                     /locus_tag="VHH53_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX2289958.1"
                     /translation="WLARRGVSVAPFKAQNMSNNSVVTPDGGEIGRAQALQAAACGIQ
                     PRVRLNPVLLKPGSDRCAQVVVLGQANGTVSAASYRNRKAELLDVVVGALSALRAEHD
                     VVVCEGAGSPTEINLRDTDIANMGLARAAELPVLVVGDIDRGGVLAHLFGTLALLAPA
                     DQALVAGFVINKFRGDPGLLAPGLEQLRALTGRRSYGVLPFHDGLWLDAEDSLAHTAD
                     AAVGRCAPPHGSQWLRVAVVRLPRISNGTDVDALACEPGVAVRYVTEPSRLADADLVV
                     LPGSKSTVADLSWLRATGLADAVVAHAAAGRPVFGICGGYQMLGRVIVDDVESGAGVV
                     PGLGLLDLEVVFDHAKHLARPSGTALGEPVTGYEIHHSRVVHRGDVPLVTAVEDGSDG
                     GHVLGSHWHGLFENDAFRRALLRRVACLADRPGFRPAPDTSFADIRAAQLDLLGDLVA
                     AHLDTAALVELIEHGPPASLPFVPPGTAVSNLQDVV"
BASE COUNT          311 a          605 c          560 g          258 t
ORIGIN      
        1 ggtcgccctc ggcagggaag atcggtccgt tctccatgag gtcgatcagt tcgtggtccg
       61 gttcgtcaaa gttcggcgac acccagatct tgcccctcag cgaacccagt tggcgcggga
      121 tcgcagtgcg ctgtagtggc accaaccggg cgaccggctt gccgtggcgg gcgatgacac
      181 ctccacttgc gccagcagct tcgagagctg ggtcttggcc tcgtagacgt tcacctgcct
      241 gatcatcatc tcacgatact tggtcaagct ggttaaacta catcttgtag gttactgact
      301 gcagtacctg gtgggacgaa gggcaagcta gctgggggac catgctcgat cagttcaacc
      361 agcgcggcgg tatccagatg ggccgcgacg aggtcgccga gcaggtcaag ttgcgccgcc
      421 cggatatcag cgaagctggt gtcgggcgcc gggcgaaaac cagggcggtc ggccaggcag
      481 gccactcggc gcagcaaggc gcggcggaag gcatcattct cgaagagccc gtgccagtgg
      541 ctacccagca cgtggccgcc atcagaaccg tcctcaacgg cagtcaccag cgggacatca
      601 ccgcggtgca cgacccggct gtgatggatc tcgtagccgg tcactggctc gcccagcgcc
      661 gtcccggacg ggcgggctag gtgtttggcg tggtcgaaga ccacctcaag gtcgagcagc
      721 cccagcccgg gcaccacccc ggcacccgac tcgacgtcat cgacgatgac gcgccccagc
      781 atctggtagc cgccgcagat gccgaacacc ggtcgtccgg ccgccgcgtg cgcgactacc
      841 gcgtcggcca gcccagtcgc gcgcaaccag gaaaggtccg cgacggtgga cttcgagccg
      901 ggcagcacta ccaggtcggc atcagccagc cgggagggct cggtgacgta acggaccgcc
      961 acccccggtt cacaggccaa cgcgtcgaca tcggtcccat tggagatccg cggcagccgc
     1021 accacggcga cccgcagcca ctgggaacca tgcggcggcg cgcaacgccc caccgctgcg
     1081 tcggcggtgt gcgccaggga gtcctcagcg tccagccaca agccatcgtg aaacggcagc
     1141 acgccgtagc tgcgccggcc ggtcagcgcc cgcaactgct ccagacctgg tgcgagcaag
     1201 cctggatcgc cgcgaaactt gttgatcacg aatccggcta ccagcgcctg gtcagcaggt
     1261 gccagcaagg ccagggtgcc gaacagatgc gcgagcacgc cgccacggtc gatgtccccg
     1321 actaccagta ccggaagctc ggcggcgcga gccaagccca tattggcgat gtcggtgtct
     1381 cgaaggttga tctcggtggg cgatccggcg ccctcacaga ccaccacatc atgctcggcc
     1441 cgcagcgcgg acagtgcacc gacgacgaca tcgaggagct cagccttgcg gttccggtag
     1501 gacgcagccg acaccgtgcc gttggcctgc ccgagcacca ctacctgggc gcaccggtcg
     1561 ctgccgggtt tgagcagcac cgggttcagc cgcaccctgg gttggatccc gcacgccgcg
     1621 gcttgcagcg cctgggcccg gccgatctcc cccccatcgg gggtcaccac cgagttattg
     1681 gacatgttct gtgctttgaa cggcgccacc gacacccccc gccgggccag ccac
//
LOCUS       DATADN010000553         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_523532, whole genome shotgun sequence.
ACCESSION   DATADN010000553 DATADN010000000
VERSION     DATADN010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523532"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VHH53_07150"
     CDS             complement(<1..367)
                     /locus_tag="VHH53_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2289959.1"
                     /translation="MADKTSESGFAIKPVYQADDIADGLADRLGQPGEFPFTRGVYPA
                     MYTQRPWTMRQYAGFGTAAESNARYHQLLQAGQAGLSVAFDLPTQMGYDSDEAIAHGE
                     VGKVGVAIDSIEDMRRLFDG"
     gene            377..>1925
                     /locus_tag="VHH53_07155"
     CDS             377..>1925
                     /locus_tag="VHH53_07155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2289960.1"
                     /translation="MAHTSGNHNAGHHVAVTNPSPRLVAGRYRLSGVLGRGTMGTVWS
                     AHDEVLGRPVAVKEVLLPPGIPEAEADAVRERTMREARTIAALSHPNVVTLYDVAREG
                     AAPFVVMELVASRSLAELLLAGPLPPQQVIAVGAAVAAALQAAHAAGITHRDVKPGNV
                     LVAHDGRIKLTDFGIARNPADQTLTATGLMLGSPAYIAPEIASGCPVSPAADLWGLGA
                     TLFSAVEGRPPYDAGTAMATVASVVQDEVPRASCSGPLAEVISGLMTKDPAHRMPLSQ
                     VRQLLGAPLEPTDPESTPRPAIAATSTPITIPKDVQQADTSRRRTGQLAADPGPLPFT
                     VASTSKTSAAAHRVQSTGGGRRMLLWTLALLLFLGAATTAFALTRAAAGRSALPTLEA
                     PAVLGARTDDAVLTRRVVQVVQADGSRAARFSISVPQDWAEFREQRALGNNSSTIMRF
                     VSPDGSEVIAVERVTGVLPSTVGDQYLTNYLRRLAQGVPELVETQRQEMSTGANDAAF
                     RTQEGGDV"
BASE COUNT          311 a          676 c          648 g          290 t
ORIGIN      
        1 taccgtcgaa cagccttcgc atgtcctcga tcgagtcgat ggcaaccccg accttgccga
       61 cctcgccgtg cgcgatggcc tcgtcggagt cgtaacccat ctgcgtcggc agatcgaaag
      121 ccaccgacag gccggcttgc ccggcctgca gcagctggtg atagcgggcg ttggattcgg
      181 cggcggtacc gaatccggcg tactggcgca tcgtccaggg acgctgggtg tacatggcgg
      241 gatacacccc gcgggtgaac gggaactccc ccggctgacc aagccggtcg gccagaccgt
      301 ccgcgatgtc gtcggcctgg tacaccggct tgatcgcaaa gcccgattct gaagtcttgt
      361 cggccatcag gtctgtatgg cacacaccag cggcaaccac aacgcagggc atcatgtggc
      421 ggtgaccaac ccttcacccc gcctggtggc ggggcgctac cggctcagtg gcgtgctggg
      481 ccggggcacg atgggcacgg tgtggtcggc ccatgacgag gtgctcggcc ggccggtcgc
      541 ggtcaaggag gtgctgctgc caccgggtat cccggaagcc gaggccgacg ccgtgcggga
      601 gcggacgatg cgggaagcca ggaccatcgc cgcgctgtca caccccaatg tggtcaccct
      661 ctacgacgtg gcacgggagg gcgccgcacc gttcgtggtg atggagttgg tggcttcccg
      721 cagcctggcc gagctgctgc tggccggacc cctgccgccg cagcaggtga tcgccgtggg
      781 agccgcggtg gcggcggcgc tgcaggccgc gcacgcggcg ggcatcacac accgggacgt
      841 caagcccggc aacgtgctgg tggcccacga cggccgtatc aagctcactg acttcgggat
      901 cgcccgcaat ccggccgacc agacgttgac cgcgaccggc ctgatgctgg gctcccctgc
      961 ctacattgct ccagagatcg cctccggttg cccggtcagc ccggcggcgg acctgtgggg
     1021 cctcggcgcg acgctgttct ccgcagtcga gggcagaccg ccctatgacg cgggcactgc
     1081 gatggcaaca gtcgcctcgg tggtgcagga cgaggtgccc agggcatcgt gttccggacc
     1141 gctcgccgag gtgatcagcg gattgatgac caaagatcca gcacaccgga tgccgctgtc
     1201 gcaggtgcgc caactgctcg gcgcacccct cgagccaacg gaccccgagt ccactccgcg
     1261 cccagccatc gccgcaacga gcaccccgat aaccatcccg aaagacgtcc agcaggccga
     1321 cacatcgcgc cgacggaccg ggcagctcgc tgccgacccg ggtccgctgc ccttcaccgt
     1381 cgcgtccacg agcaagacct cggcggcggc acaccgagtc cagagtaccg gtggcggacg
     1441 tcgaatgctg ctgtggacgt tggcgttact gcttttcctc ggtgccgcaa ctactgcttt
     1501 tgccctcacc cgagccgccg ccggtaggtc cgccctgccc accttggagg cccccgccgt
     1561 attgggtgcc cgcaccgacg acgccgtgct gacgcgccga gtggtgcagg tagtccaggc
     1621 cgacggctcg cgggcggctc ggttcagcat cagcgtgccg caggactggg cggagttccg
     1681 cgagcaacgg gcgctcggca acaactcgag caccatcatg cgtttcgtct ctccggacgg
     1741 ctcggaggtc atcgccgtgg agcgggtcac cggcgtgcta cccagcaccg tcggagacca
     1801 gtacctgacc aactatctgc gcaggctcgc ccagggcgtg cccgaactcg tcgagaccca
     1861 gcggcaggag atgtccaccg gagcgaacga tgccgccttc cgaacccagg agggcggcga
     1921 cgtgc
//
LOCUS       DATADN010000554         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_208094, whole genome shotgun sequence.
ACCESSION   DATADN010000554 DATADN010000000
VERSION     DATADN010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208094"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VHH53_07160"
     CDS             complement(<1..713)
                     /locus_tag="VHH53_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2289961.1"
                     /translation="MPGPHRALISRRSVLLGGAAGLGTAVLGGALSGCAGSQRPPDPF
                     TLGVASGEPSAAGVVLWTRLAPRPLAEDGLGGMAPRPVEVEWELASDEQFNRVQRRGT
                     ETARPDTAHSVHVELDGLAPGREYFYRFRSQGFLSPAGRTRTAPGSAAAGGPLTMCFA
                     SCSQYEHGFFTAYRRLAEDHPDLVLHLGDYQYEYTANDYVADGGNVRDHAGPATVTLA
                     NYRQRHAQYKTDPDLQAAHA"
     gene            1218..>1702
                     /gene="shbA"
                     /locus_tag="VHH53_07165"
     CDS             1218..>1702
                     /gene="shbA"
                     /locus_tag="VHH53_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor ShbA"
                     /protein_id="HEX2289962.1"
                     /translation="MKAPAEDDLDGLVRAALAGDRGAVDGVLRRIRPLVLRYCRARVG
                     SQEKSFASADDVAQEVCLAVFTALPSYRYQGRPFLAFVYGIAAHKVADARRSAARNRA
                     EPFPDVPDTSEPVDGPEVQLLQAEVAERMTKLLDTLSKRQRDILVLRVVVGLSVEETA
                     EA"
BASE COUNT          272 a          562 c          585 g          283 t
ORIGIN      
        1 gcgtgcgcgg cctgcagatc cgggtcggtc ttgtactggg catgccgctg ccggtagttg
       61 gccaacgtga cggtcgccgg tccagcgtgg tcacggacgt tgcccccgtc ggccacgtag
      121 tcgttggcgg tgtactcgta ctggtagtca cccaggtgca gtaccaggtc cgggtggtcc
      181 tcggcgaggc ggcgataggc ggtgaagaag ccgtgctcgt actgcgagca ggaggcgaaa
      241 cacatggtca gcggcccgcc agcagcggcg gatcccggag cagtgcgggt ccggccggct
      301 ggcgacagga acccctggct gcggaaccgg tagaagtact cccgtcccgg cgccaacccg
      361 tcgagttcga cgtgcacgct gtgtgccgtg tctgggcgcg ccgtctcggt gccgcgccgt
      421 tgcacccggt tgaactgctc gtcggaggct agttcccatt ccacttcgac cgggcgcggc
      481 gccatcccgc ccagcccgtc ctcggccagt gggcgcgggg cgagccgagt ccagagcact
      541 acaccggcag cactcggttc gccggaggcg acccccagcg tgaacgggtc cggcgggcgc
      601 tggctcccgg cgcacccgga cagcgcgccg ccgaggactg ccgttcccag ccctgcggcg
      661 ccgccgagca acacactgcg ccgactgatc aatgcacggt gtgggccggg cacgcgtcac
      721 ctcctactcg ctcgtcggtt ctggagtcga tggcaactcc gctctggtgg cctgcgccgg
      781 agcacgtgag tgacgagttg gccacgaacc ggtgaacagg tcgggcggtc tcgtttccgc
      841 cggtgggacg gccacgatca ccccacagac gtaccaaata tccgggcact accgcatttg
      901 atgtaccggg actgcacgac ctggggtacg ccgaagatga tccgtggaag ggatggccgg
      961 ccgctgcgcg cccggcatat taaacaccca gcaaggctgc tgctccgcgc accggtcttt
     1021 gtcaacagag tccgcaatac cgccgaaact tgaacacggt ggctggtcga tcagcgaagg
     1081 ggcaccacgt ggctacgctt tcactatgtg acgtgcagac gtcgttggct ccagaaggag
     1141 accatgcagt cgacgatgtc cagccactga ccgatcgatt ccagaatgcc accacccttc
     1201 ttcagggtca gggccagctg aaggccccag ccgaagacga cctcgacggc ctggtccgtg
     1261 ctgccctcgc gggcgatcga ggcgctgtcg acggtgtgct ccgccggatc cgtcctctcg
     1321 tgctgcggta ctgccgcgcc cgcgtcggca gccaggagaa gtcattcgcg tcggcggacg
     1381 acgtagccca ggaggtgtgt ctcgcggtgt tcacggccct gcccagttac cgctatcagg
     1441 gccgaccgtt tcttgccttt gtgtacggaa tcgcggcgca caaggtggca gatgcccgcc
     1501 ggtcagcggc gcgcaatcgc gccgagccgt tcccggatgt tcccgatacc tccgaaccgg
     1561 tcgacggtcc ggaggtgcag ctgctgcagg ccgaggtggc cgagcggatg accaagctgc
     1621 tggacacgtt gtcgaagagg cagcgggaca ttctcgtcct gcgggtcgtg gtggggctgt
     1681 cggtagagga gaccgccgag gc
//
LOCUS       DATADN010000555         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1130041, whole genome shotgun sequence.
ACCESSION   DATADN010000555 DATADN010000000
VERSION     DATADN010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130041"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1338)
                     /locus_tag="VHH53_07170"
     CDS             complement(145..1338)
                     /locus_tag="VHH53_07170"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX2289963.1"
                     /translation="MSACPSLPIPLRDVCDGPPQAGPACAAADPWGPDLLVALSRVPD
                     PRNSRSVRHRLVTVLAAEVCAVLAGARSYVGIAEWAHDLPVSVRVRLGIGRRAPSEST
                     FRRVLQTVDAHALDTAVSSWLVERSGLGRKANQALRAVAVDGKTARGSRAPDQPARHL
                     FAAFEHASGVVLGQTEVDDKSNEITAFAPLLDRIDLTGTVITADALHTQDRHATYLHQ
                     RGGHYVFIVKNNRPKLRTQLAGLPWRDIPALDLSQDKRHGRVESRTLKLTAIGSGIIF
                     PHAQLAAQIVRRRRPTTSTATGTWHTETVYAVTDLGFGDIRADQLAEIIRDHWSIENK
                     LHWIQDVTFAEDHSQIRTGNGPAVMATLRNFALSRHRLGDHTNIAAACRHTSRHPIRA
                     ADLLT"
BASE COUNT          277 a          486 c          578 g          288 t
ORIGIN      
        1 ccgcaatcgg gtagccaagc ccagcaagtt caccttggat ccgccggtaa ccccaggagg
       61 agttctccga ggccagccgc agggcggcat tgtcaggttg gccgctgggg gaagcccagg
      121 gccccggcat agttgatctg caatttatgt gagcaggtca gcggcccgaa tgggatggcg
      181 gctggtgtgc cggcaggcgg cggcgatgtt ggtgtggtcg cctaggcggt ggcggctgag
      241 ggcgaagttg cgcagggtgg ccatgaccgc gggtccgttg ccggtgcgta tctgggaatg
      301 gtcctcggcg aaggtcacgt cttggatcca gtgcagtttg ttctcgattg accagtggtc
      361 gcggatgatc tcggccagct ggtcggcgcg gatgtcgccg aagccgaggt cggtgaccgc
      421 gtagacggtc tcggtgtgcc aggtacctgt cgcggtgctg gtggtcgggc gtcggcggcg
      481 cacgatctga gcggcgagct gggcgtgcgg gaagatgatc ccggagccaa tggcggtgag
      541 tttgagggtg cgtgactcca cccggccatg ccttttgtct tggctgaggt ccagggccgg
      601 gatgtcgcgc cagggcagcc cggccagttg ggtgcgcagt ttcgggcggt tgtttttgac
      661 gatgaataca tagtggccgc cgcgttggtg caggtaggtc gcgtggcggt cctgggtgtg
      721 cagcgcatcg gcggtgatga cggtgccggt gagatcgatc cgatccagca gtggcgcgaa
      781 ggcggtgatc tcgttgctct tgtcgtcgac ctcggtctgg cccagcacga caccgctggc
      841 gtgttcgaac gcggcgaaca gatgccgtgc tggctggtcg ggggcacgag aaccacgggc
      901 ggtcttgccg tccacggcga ctgctcgcag cgcctggttc gcctttcgcc ccaggcccga
      961 acgctcgacg agccaggacg acaccgccgt gtccagcgcg tgcgcgtcga cggtttgcag
     1021 gactcggcga aacgtggact cactcggggc gcgccggccg atcccaagcc ggacccgcac
     1081 cgaaaccggc aagtcatgag cccattccgc gatccccaca tagctgcgcg ctccggccag
     1141 caccgcgcac acctccgcgg ctaacaccgt caccaaccgg tgccgcacgc tccgactatt
     1201 acgagggtcg gggacccgag acagcgcgac cagcagatcc gggccccatg gatccgcagc
     1261 tgcgcaggcc ggaccagcct gcggtggccc gtcacagaca tcgcgaagag gtatcggcaa
     1321 ggatgggcaa gcggacacgg cgacactccg atgatcaagc ctggggtcag agccgccatg
     1381 atcactcgtg ctgccgtgtc cgccctacta catcatcacc ggcgtgtcgc tgtgtagaca
     1441 tgccgagtaa tcggactatg ccggggccct gggggcacct ggcagtggcc gaaccggttg
     1501 gcgttccgat tttcagaagt ctggataaca gttcgccgaa taccgttaac gtccgcgacc
     1561 tgcatcgctg cattaatccg aacgccaatg aacggaggtg aacgtagacg gatgagacca
     1621 aagatgaga
//
LOCUS       DATADN010000556         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_208107, whole genome shotgun sequence.
ACCESSION   DATADN010000556 DATADN010000000
VERSION     DATADN010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208107"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VHH53_07175"
     CDS             complement(<1..504)
                     /locus_tag="VHH53_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289964.1"
                     /translation="MDVAGSGEVNGFAQRRRARLAAALSGLDWLVAETCTSPALSPDG
                     ATLAVVSDRDGTPGVWLIPLVQPGEPVRLDTGEDYVRVVSWSPDGEWLCLTTASGGGE
                     RTLVRALRPDGSDARVIAGTPAGMASIGCWQPGGHMVGLAESSASDPTSLDTYAVDVC
                     SGRRRQLA"
     gene            complement(505..>1979)
                     /locus_tag="VHH53_07180"
     CDS             complement(505..>1979)
                     /locus_tag="VHH53_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HEX2289965.1"
                     /translation="EVLLKAYHRWGTSCVDHFMGMFAFAVLERDSGRLVLGRDRLGIK
                     PLYLSQTASRLRFASALPALLAAGEVNTAIDPIGLHHYLSFHSVVPAPHTILAGVEKL
                     PPATVRTVYPDGRREDREYWRPAFSRGGCGLDIVDWPGAVLDALRLAVQRRMVADVPV
                     GVLLSGGIDSSLVVALLAEGGWPGLRTFSVGFEAAGGESGDEFIYSDQVAAHFDTDHQ
                     KIMVTSDRLLPAVDAAIDAMSEPMVSHDCVAFYLLSEEVSRSVTVVQSGQGADEVFAG
                     YDWYPPLAGVPQGCAAAAYGKVFVDRPHTELADLLEPEWLTGSDYSQAFIDRHFADHG
                     ADTALDAALCLDTTVMLVDDPVKRVDNMTMAWGLEARVPFLDHELIELAAACPSELKL
                     AAGGKGVLKAASRGVVPDTIIDRPKGYFPVPAIRHLSGSLLDRVRDAITDPVAKDRGL
                     FRPDWLQRMLADPNTERTTLGSNALWQVALLEMWLQGRGV"
BASE COUNT          349 a          701 c          644 g          285 t
ORIGIN      
        1 cgccagctga cggcggcgcc cggagcacac gtccaccgcg taggtgtcaa ggctggtcgg
       61 atcgctggca ctgctctccg ccagacccac catgtgcccg ccgggctgcc agcagccgat
      121 cgacgccatc ccggcggggg taccggcgat gacccgggca tcggaaccgt ccgggcgtag
      181 cgcacggacc agggtccgct cgccgccgcc ggacgctgtc gtcaggcaca gccattcacc
      241 gtcgggggac cagctgacca cccgtacgta atcctcgccg gtgtccagcc gcaccggctc
      301 gccgggctgc accagcggga tcagccagac tcccggcgtg ccgtcgcgat cagagaccac
      361 ggccagggtg gcgccgtccg gggacagcgc gggtgaggta caagtctcgg cgacgagcca
      421 gtcgaggccg gacagtgcag cggcgagccg ggcgcggcgc cgctgtgcaa agccgttgac
      481 ctctccggag cccgccacat ccacctatac ccccctgccc tgcagccaca tctccagcag
      541 ggcgacttgc cacaaggcgt tggaccccag tgtggtgcgt tcggtgttcg gatctgcaag
      601 catgcgctgc aaccagtccg ggcggaagag cccgcggtcc ttggccaccg gatcggtaat
      661 cgcgtcgcgc acccggtcga gcaacgatcc ggacaggtgc cggatggccg gaaccgggaa
      721 gtaacccttg ggccggtcga tgatcgtgtc cggtaccacg cctcgactgg ccgccttgag
      781 tacgcccttg ccgccagcgg cgagcttgag ctcggatggg caggcagcgg cgagttcgat
      841 cagctcgtga tccaggaacg gcacccgggc ttccagcccc cacgccatcg tcatgttgtc
      901 caccctcttg accgggtcgt ctaccaacat gaccgtggtg tccagacaca acgccgcgtc
      961 cagcgccgtg tcggcaccat gatcagcgaa gtgcctgtcg atgaatgctt ggctgtagtc
     1021 cgagccggtc agccactccg gctcgagcag gtcggccagc tcggtgtgcg gacggtcgac
     1081 gaacaccttc ccgtacgccg ccgcggcgca gccctgtggc acgccggcaa gcggtgggta
     1141 ccagtcgtat ccggcgaaga cctcgtctgc accctgcccg gactgcacca cggtcaccga
     1201 gcgggagacc tcctctgaga gcaagtagaa cgcgacacag tcatggctga ccatcggttc
     1261 gctcatcgcg tcgatggccg cgtccaccgc cggcagcagc cggtctgacg tgaccatgat
     1321 cttctgatga tcggtgtcga agtgggccgc gacctggtcg gagtagatga attcatcgcc
     1381 ggactcgccg ccggcagcct cgaatccgac actgaacgtg cgcagccccg gccaaccgcc
     1441 ctcagccagc aacgccacta ccaggctgga gtcgataccg ccggagagca ggacacctac
     1501 cggaacgtcg gcgaccatgc gccgctgcac cgcgaggcgc agcgcatcca gcaccgcgcc
     1561 cggccagtcg acgatatcga gaccgcaacc accccgcgag aatgccggcc gccagtactc
     1621 cctatcctcg cgacggccat ccggatacac agtgcgcacg gtggccgggg gtagcttctc
     1681 gactccggcc agaatcgtgt gcggagccgg cacgacggag tgaaagctca ggtagtggtg
     1741 cagcccgatc ggatcgatcg cggtgttcac ctcacccgca gcgagcagcg ccggcagcgc
     1801 ggaggcgaag cgtagccggc ttgcggtctg cgagagatac agcggtttga tcccgagccg
     1861 gtcgcggccg agcaccagcc gcccgctgtc ccgttcaagg acggcgaagg cgaacatgcc
     1921 catgaaatgg tcgacgcagc tggtacccca ccggtggtag gccttgagca gcacctcac
//
LOCUS       DATADN010000557         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_670900, whole genome shotgun sequence.
ACCESSION   DATADN010000557 DATADN010000000
VERSION     DATADN010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670900"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..801)
                     /gene="thpR"
                     /locus_tag="VHH53_07185"
     CDS             complement(253..801)
                     /gene="thpR"
                     /locus_tag="VHH53_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX2289966.1"
                     /translation="MRLFVALTPPGVIVEELSISIAALRELAPGLRWTRPEHWHVTLA
                     FLGEVGDDVVPQLARRLNRAAARYPPLSLSLGGGGRFGYQVLWTGVHGDRDELRRLAG
                     SVQAAARRCRLPVAQRPYRPHLTLARAGTATDLRPLVERLTSWQGQPWAATRVYLVRS
                     RLGAGLNGSALHEPIAGWPLGQ"
     gene            1118..>1716
                     /locus_tag="VHH53_07190"
     CDS             1118..>1716
                     /locus_tag="VHH53_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289967.1"
                     /translation="MAQESLVQTYKTPLRPQVCATDAVEVLSAPDHLARAAVEALGWD
                     GVVLPPAVLLGRRVVVVADLIDEVHEQRTMSGWAPVTDRMTVSTWNWVEMQQLAPPPA
                     VTLRGVIAPARHWRTGLTGAAPFAGLCATALLLPAHIARNVECLAHAEYYGPTVLAAA
                     GPHVHHIDRLGVDIVGPCGGQHGRSVVLGVRKALNVSRNV"
BASE COUNT          303 a          602 c          546 g          265 t
ORIGIN      
        1 ccgcggacta cttagatgca ctaaggtaac tacttagttt tgatgttgac atggctaccg
       61 agcggtgtcc accaataaca gtgtgtcgac cgtattcggc gaccgggccc gcgaagggta
      121 agccataccg atcacttcgc cgctcagtac ctagcagaca gcgtcgatcc cccagaattc
      181 attgggcgaa tccgggcatc agccttgatc aggaacgtaa gcgagcccac aaggtgatca
      241 agaaactttc ggttactggc cgagcggcca gccggcgatc ggctcgtgca gcgcagaacc
      301 gttcaatccc gcgccaaggc ggctacggac caggtacacc cgggtggccg cccacggctg
      361 cccctgccag gacgtgagcc gttcgaccag cggccgcagg tcggtcgctg tgcctgccct
      421 ggccaacgtg agatgcggac ggtacgggcg ttgcgctacc ggtaggcggc agcgccgggc
      481 cgcggcctgc accgaaccgg ccagcctgcg taactcgtcg cgatccccgt gcaccccggt
      541 ccacaacacc tggtaaccga agcggccgcc gccgccgagc gacagggaca gaggtgggta
      601 ccgagcggcc gcgcggttga gccggcgggc cagctggggg acaacgtcgt cgcccacctc
      661 accgaggaag gccagcgtca cgtgccaatg ctctggacgg gtccaccgca aacccggtgc
      721 cagctcgcgc agcgccgcga tgctgatact cagctcctcg acaatcactc caggtggcgt
      781 cagcgccacg aacagccgca ccctggcccc ttcccagccc tgcccgtcag gccggctaca
      841 caatgtccgg catccaccgt ccgggcaacc gcaggcgatt cacacagccg cccgacgttg
      901 cgcttaccca ctggttaagc ggtcaccggt ggggcttagc ggtcggtggc accgtcgaac
      961 ggaacgcggc cgaccagtca gcgcggcagt accgccaccg gacgccacaa cggccctgct
     1021 tccaccaccg gacaactacc tcctggtaat tcttactgaa cgctgcgcgg cccagctgcg
     1081 cgacgaacat cctccagatg atgggagaaa gtgccccgtg gcacaggaat cgctggtgca
     1141 gacctacaag accccgctga ggcctcaggt ctgtgccacc gacgccgtcg aggtgctctc
     1201 cgcaccggac catctggctc gcgccgccgt cgaggcactg ggctgggacg gcgtggtgct
     1261 gcccccggcc gtattactcg gccgccgtgt ggtggttgtc gccgacctca tcgatgaggt
     1321 gcatgagcaa cggaccatgt cgggctgggc tccggtgacc gaccggatga cggtatccac
     1381 ctggaattgg gtcgagatgc agcagctcgc gccgcctccc gcggtgacgt tgcgcggtgt
     1441 catcgcacct gcgcggcact ggcgcaccgg gcttaccgga gccgcgccgt tcgccggcct
     1501 gtgcgcgacg gctctgctgc tgccagcaca cattgcgcga aacgttgagt gcctggcgca
     1561 cgccgagtac tacggaccga ccgtgctggc cgccgcaggg ccccacgtgc accacatcga
     1621 ccgcctcggg gtcgacatcg tggggccctg cggcggccag cacggtcggt ccgtagtact
     1681 cggcgtgcgc aaggcactca acgtttcgcg caatgt
//
LOCUS       DATADN010000558         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_52239, whole genome shotgun sequence.
ACCESSION   DATADN010000558 DATADN010000000
VERSION     DATADN010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52239"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1308)
                     /locus_tag="VHH53_07195"
                     /pseudo
     CDS             complement(1..1308)
                     /locus_tag="VHH53_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335644.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
BASE COUNT          326 a          559 c          470 g          183 t
ORIGIN      
        1 caggtgttgg cgcacgtgcc acctgggaca gccgggggat ggacaggaag ctcagggtgc
       61 cctcgaccac cactcgccac gaagcgggtt ttccctccga ttctgaccgc acctgctgcg
      121 cgtgcacccc tgaccagatg acccggagca gcaccagcag cagcgagagc cctagcccga
      181 tgaggactcc ggtcagcagg ttgacaaaca ccacaccggc cgtcgtgacc agatacaccg
      241 tcagctcacc ctgtctgtgg gccgagcgca cgtccgcgag cttgaccaac cgcaggccga
      301 tcaccaccag cagcccggcg agcgcagcca tcgggatcag ctcgatcagg ccgacgagca
      361 ggaccgagaa cagcagtacc cacccgccgt gcagcaccgc cgaagcgcga gtgccagctc
      421 ctgcggcgat attggtggaa ctccggacga tcacaccggt caccggaagg ccacccacca
      481 gccccgacac ggtgttcgcc gcgccttggc cgatcagctc tcggtcaagg ttggtcctgg
      541 tgccgcccat tttgtccgtc gccaccgcgc acagcaggct ttccacactg gcgatcaacg
      601 cgacggtcag cacgccggtg agcacgccga cccacatgcc gcccggcacc tgcggcaggt
      661 gcagcgaatc gagcagcgag ccggggatca ctactcgctc caggtcaagc gcgagcccag
      721 tggctaatgc ggtggcggcg accacggcca ccagtggccc tgggatctgg cgcagcggga
      781 gcggcaaccg cggccacaac agcaacacgg caatcaccgc caggcccacc accgcggcgg
      841 gatcgtggat atcggcgagc tggccgggca ggtcgacgag gttagtgatc gcgtcgctac
      901 ccggtgcacc gccgagcaac acatgcaact ggcccagcac aatggtgatg ccgataccgg
      961 ccagcatcgc gtgcaccacc gctggtgaga tcgccagggc cccccgggcc acccggctga
     1021 ctccaaacag cacctgcagc agcccagcag ccaccgtgat gcacacgtga tgccccaacc
     1081 aaaccgccca accaggtcgg ccaccaccac ggtgagtccc gccgcaggac cgctgacctg
     1141 caggggtgag ccaccaagca acccggccac aattcccccg acaacggcgg cgatgagccc
     1201 ggccatcaca ggcgcacctg aggccacggc aatgcccagc gacagcggta ccgcaaccaa
     1261 gaagaccacc agcgaagcag gcaagtcgtg acgcaacagc gtgtgcagcc cgcctggtgc
     1321 cgatgagggc ggcgctacgg gcgtgggcga agcggcaggc accaagcaca acctctgcag
     1381 atcgataggc gggcagtcgg ctgcgggtcc ggcgcacgcg gcaccgtcac gtcgcttcgt
     1441 tgcaccccca cggccaaaga ctatacgaac ggcgatctac taccgatcag ctcaatcccg
     1501 ataacgataa tcggaatcaa gccgaacgtt ggccaggg
//
LOCUS       DATADN010000559         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_671330, whole genome shotgun sequence.
ACCESSION   DATADN010000559 DATADN010000000
VERSION     DATADN010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671330"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..782
                     /locus_tag="VHH53_07200"
     CDS             15..782
                     /locus_tag="VHH53_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX2289968.1"
                     /translation="MRVALSTASVWPESVAVGFELAAELGYDGVEVMVWGDPASQDVA
                     ALAKLAVRHRMPVLAVHAPCLLITQRVWAADPAVRLERTVAAAIELGAGVVVVHPPFR
                     WQVRYATGFAQQISALEASSGLTIAVENMFPVRRGRFEMSSYAPSPDPTDVGYSHYTL
                     DLSHTATARVDAVAMAQRMGSRLAHLHLADGSGASRDEHLVPGRGSQPCAEVCRALVN
                     GGFTGTVVLEISTRRARTRPERRAALTEAVLFARLNL"
     gene            complement(850..1293)
                     /locus_tag="VHH53_07205"
     CDS             complement(850..1293)
                     /locus_tag="VHH53_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289969.1"
                     /translation="MLNNLRVIRGNKLSALAEVLHRVAAIVHDIRHEPVSFVQGSPGP
                     IDELGLDGFPAPGVAVAGRRLQLADVEPIAPIGDLAQVLQGVALATARLHLTFVLGSE
                     SLLQAGAPTPAGIHYHEDHNRGQHDNTNDDQEDDGVHGMPPHPFE"
     gene            1382..1876
                     /locus_tag="VHH53_07210"
     CDS             1382..1876
                     /locus_tag="VHH53_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289970.1"
                     /translation="MNTPEEHHEQQQRSQAPGGRVRRFLDSDDREQPVGAQTGHPSSP
                     DLNHPGGGAQREPVAPSGAPAHVEQPETAATDDTARDGGAQRVGLINDVAGLRDEWQR
                     VQATFVDDPQRAVHEAGVLIDRTLDEIRMNVGSGHPSETTSTEDLRVRFQRYREFFQR
                     LLSA"
BASE COUNT          354 a          667 c          734 g          377 t
ORIGIN      
        1 cgccggcaga cccgatccgg gtggccctgt ccaccgcctc ggtatggccg gaatcggtcg
       61 ccgtgggatt cgaactggcc gccgagctcg gctacgacgg cgtcgaggtg atggtctggg
      121 gtgatccggc cagccaggat gtcgccgccc tcgcgaagtt agcggtccgc caccggatgc
      181 ccgtgctagc cgtacatgcc ccgtgtttac tgatcactca gcgggtgtgg gcggcagatc
      241 cggcggttcg gctggagcgc accgttgcgg cggccatcga gttgggagcc ggtgtcgtgg
      301 tggtgcaccc cccgtttcgt tggcaggtgc gctacgccac cgggttcgca cagcagatct
      361 ccgcgctgga agcctcatcc gggctcacta tcgcggtgga gaacatgttc ccggtgcgtc
      421 gtggccggtt cgagatgtca agctacgcac cgtcaccgga tccgacggat gtcggatatt
      481 cgcactacac gctggatctg tcccataccg ccaccgcccg agtcgacgcg gtggcgatgg
      541 cgcaacggat ggggtcacgg ttggcgcacc tgcatctggc tgatggcagc ggtgcttccc
      601 gtgacgagca tctggtgcct ggtcggggta gtcagccttg tgctgaggtg tgccgggcac
      661 tggtgaacgg cggattcacc ggcactgtcg tgctcgagat cagcacccgg cgggcccgta
      721 cccgcccgga gcgccgcgcc gcgctcaccg aggccgtgct ttttgcgcgc ttaaatctat
      781 aaatagcgat aggccgattg ggtgtctctt gggtcgacgt cccgctagcc gggtatgtaa
      841 cgaaatgggt cactcaaacg ggtgtggagg catgccatgg acaccgtcgt cctcctgatc
      901 atcattggtg ttatcgtgct ggccgcggtt gtgatcttcg tgatagtgga tacccgccgg
      961 cgtcggcgcg ccggcctgca ggagcgattc ggacccgagt acgaacgtga ggtgcaggcg
     1021 cgcggtagcg agcgcgacgc cctgcagcac ttgggcaagg tcgccgatcg gcgcgatcgg
     1081 ctcgacatcc gccagctgga gcctgcggcc cgcgaccgct acacccggcg ctgggaagcc
     1141 gtccagaccc agttcgtcga tcggcccggg gctgccctgg acgaagctga ccggctcgtg
     1201 acggatgtca tgcacgatcg cggctacccg gtggaggact tcggcgagcg cgctgagctt
     1261 gttgccgcgg atcacccgca ggttgttgag cactaccggg ccctgttcga cgagctcgtg
     1321 catggccgtc aagacacgtc gagatagccc aacactaggt atcacggccg gaggcttcag
     1381 gatgaacacg ccggaggaac accacgagca gcagcagcgg tcgcaggctc cgggtgggcg
     1441 ggtgcgtcgc ttcctcgaca gtgacgacag ggagcagcca gtcggggcgc agaccggtca
     1501 tccgagtagc ccggatctga accaccccgg cggtggcgcg caacgagagc cggtcgcccc
     1561 cagcggcgca ccggcccacg tcgagcagcc ggagacggcc gctaccgacg acactgcccg
     1621 cgatggtggt gcgcagcggg tgggactgat caacgatgtg gccggcctac gcgacgagtg
     1681 gcagcgagtg caggcaacgt tcgtcgacga tccgcagcgc gcggtacatg aggccggcgt
     1741 gctgatcgac cggacgctgg acgagatccg catgaacgtg ggaagtgggc acccgagcga
     1801 gacgacgtcg accgaagatc tgcgggttcg cttccagcgt tatcgagagt tcttccagcg
     1861 cctgctgtct gcgtgatctc aacccggcac cggtagcgcg tgccgcctgt actccttgtc
     1921 accgacgccc taccgacatg cgcttatgtc tgtgagcaac ccgacaacag accaataggc
     1981 cgaacgggcg tatcgttgcg ctgctccaca acggggtatg tcgccactgg tgaatcacaa
     2041 ggagtgtgaa agcttgtcat gaacatggtc atcatcctcg agatactcgt cggtatcgtc
     2101 gtcgtcgcgg cggccgtgcg gttcgcggtg cg
//
LOCUS       DATADN010000560         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_377835, whole genome shotgun sequence.
ACCESSION   DATADN010000560 DATADN010000000
VERSION     DATADN010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377835"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..627
                     /locus_tag="VHH53_07215"
     CDS             409..627
                     /locus_tag="VHH53_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289971.1"
                     /translation="MGMAAILADLPDMWRSALNAHVPDPQGRCWACRDSNGVAASWPC
                     LTREVAEEAKYLHEGGLPGTFSGRHARW"
     gene            1108..>1583
                     /locus_tag="VHH53_07220"
     CDS             1108..>1583
                     /locus_tag="VHH53_07220"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX2289972.1"
                     /translation="MSLGTLVLLRHGESEWNAKNLFTGWVDVPLSIRGEREAARAGVL
                     MRAAGLLPDVLHTSLLRRAISTACLALDAADRHWIPVHRDWRLNERHYGALQGKDKSQ
                     IRARYGDAQFALWRRSYAVPPPPIEPGSESAQDADPRYAGVDVPRTECLADVVTRML"
BASE COUNT          273 a          502 c          535 g          273 t
ORIGIN      
        1 cggtacccga tttgagcgta acccgatcgg cgtacagccc cggctgtgct gtggcttccg
       61 accggtgacg ccgaggtggg ctgccgacgg ccgttcgggg acggcttgat gaggctgacg
      121 tcgagcgccc tgtaacgtca gcgacactgt tcgcgacata caggcaaccg ttgatccact
      181 gccgccgggc cgcgctgtcc ggcggctgca gtggcgtgtc gcgttcagag tgtcgcgccc
      241 ggagtgtcga ggaggaaata gccagtgcgc agtcacccac cggatacggt cggttgcaca
      301 gtgaggccat cccccgatcg gctgcttcgg ccggtgggaa tcgctggtat gaactgcgac
      361 cggactcgcc ggttgtaagg tggctcgatc tccgggaggt aaatagacat gggcatggca
      421 gcaatcctgg cggacttgcc ggacatgtgg cgcagcgcgc tgaacgcaca tgtgcccgac
      481 ccgcagggtc gctgctgggc gtgccgcgac agcaacggcg tcgccgcatc gtggccatgc
      541 ctgacccggg aggttgccga ggaagccaag tacctccacg agggtgggct gccaggcacc
      601 ttcagcggtc gccacgcccg atggtgaacc ggctgacgcc tctcatctgc cggcgagatc
      661 gcccggactt cccggcggat gaacggatct ccgaactcct accaccgcaa tactcgggat
      721 tacgctgaga ggccgaccgg tgctgatcgc tcgcgtggca agaatcagcc ccacacaatg
      781 caccgcaata tcggccgccg caatctcttg aaccccacac gtagacaggg aaccgctgta
      841 cgaccagcct ttcccggggg gcgggggaag cgagtggcca gcggttccct gcaacgacgg
      901 tggggtgtca gcagggcgtt gtcgttagca ccgtcgttac gtgttaatgt atgtgtaaca
      961 tcgtagtcac ggccagcggc cgttcgggta ttcgctaaca cgaattgatg gccataggga
     1021 tggattaagc ctgctctagc ctggccctgc cttggtaaag gcgggtcgtt tcggatcgtc
     1081 gtcacggcac ctggcaggct gcggagcatg agcctgggca ccctggtcct gctccgccac
     1141 ggcgagagcg agtggaacgc caaaaacctg ttcaccggct gggtggacgt gccgttgtcg
     1201 atacggggcg agcgtgaggc ggcgcgagcc ggcgtactga tgcgcgctgc cgggctgctg
     1261 cccgacgtgc tgcacacctc gttgctgcgc cgcgcaatca gcaccgcttg cttagcgctc
     1321 gacgccgccg accggcactg gattccggtg caccgcgact ggcggctcaa cgagcgtcac
     1381 tacggtgcgt tacagggcaa ggacaaatcc cagatccgtg cgcgatacgg cgacgcgcag
     1441 ttcgcgctgt ggcgccggtc ttatgctgtc ccgccaccac cgatcgaacc gggcagcgag
     1501 tccgcacagg acgccgatcc gcggtacgcc ggcgtcgacg tccccaggac tgagtgcctg
     1561 gccgacgtgg tgacccggat gct
//
LOCUS       DATADN010000561         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_980791, whole genome shotgun sequence.
ACCESSION   DATADN010000561 DATADN010000000
VERSION     DATADN010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980791"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..164
                     /locus_tag="VHH53_07225"
                     /pseudo
     CDS             1..164
                     /locus_tag="VHH53_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612189.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(689..>2143)
                     /locus_tag="VHH53_07230"
     CDS             complement(689..>2143)
                     /locus_tag="VHH53_07230"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX2289973.1"
                     /translation="WIHQASSTGLIASTSTYAFEACPLVVDGVMFVTGWDGWLWALDA
                     VTGEQLWRYKHAIPFDVTLCCANVNRGCAVANGKVYMVTQNAQLVAIDATNGQKVWQK
                     TIGDVRAGESASIAPLVVKDTLITGSAGGEYGVRGHIDCWDLETGEQRWRTYTVPKPG
                     EPGSETWPADGEAWQRGGANHWVTGTYDPELNLYYAGTGNPAPDFDGEVRRGDNLYSD
                     SVVALDVDTGEIKWHYQFTPHDLWDYDSTMEMTLFERDGKKLLAHFDKNGYMFVLDRT
                     NGELQHVTPFVDRIDWGVITRDGKVTPRKYPDKEGEPCHFYPGPAGAKEWTHASYNPD
                     HDLFYVPVADVGATATRRRREFKEGMPYWGAAVEVDVDNMAGSVSAFDSHGEEKWRYR
                     LDMPMAASTLSTAGNVVFAGTPTGEFMALHAETGEKLWSFQCGSGHHSSPSTYMVDGK
                     QYITVPVGWGGWLEGIIPGMSGQGHGAAVITFSL"
BASE COUNT          345 a          713 c          699 g          386 t
ORIGIN      
        1 cgcggcctgc ggcaggactt cgacgcggtc accgccggcc tcacgccgga gtggaactcg
       61 ggcaaggtcg agggaaacgt gaacagggtg aaacggataa gagggccggg tacggccggg
      121 ctggcttcgc cctcctccgc cgacagatcc tcctcgcgga ctgaccgccc tgacgctccg
      181 tgatcggacc acaagaagtg gatcagagcc gttttccacg gcggcgcagt ccgtagggct
      241 tttcagcctc gatacttgtg cacgtcgcaa acgcagtaca tgagacggca gctgaagcat
      301 gcgggcttgt cgacgcacgt gatacagggc ccgcagtgca ccggctcggt gtgccgcgcg
      361 ggatcgccgg gctcgtagtc ctccccgcag tagtggcagg gcagacgctc acctccagtg
      421 tcccgctggg gcaccgacgg ctggttcatc acatcacctt ttgtttcctt cgctgtcttc
      481 ccgatgaccg aggtgcggat tgaactgccc gccgtctgtg agactacggg ggaccttggc
      541 gtagccccgc tctccttgcc tgtcggtgcc aaagcacccg cccggtgatc cgtcgctggg
      601 acgactcgat caccgggcgg gtggaaggga aaccgccagg aagttacctc gtgtctcgcg
      661 tcagtaccac gtcagtggca cttccctgtc acagggagaa cgtgatcacc gcggcaccgt
      721 gaccctgacc agacatgccg gggataatgc cttcgagcca gccaccccag ccgaccggca
      781 ccgtgatgta ctgcttgcca tcgaccatgt aggtactcgg gctgctgtgg tgaccgctac
      841 cgcactggaa actccagagc ttctctccgg tctcggcgtg gagcgccatg aactcacccg
      901 tgggtgtgcc cgcgaacacc acgttccctg cggtgctgag ggtcgacgcg gccatcggca
      961 tgtcgagccg gtagcgccac ttctcctcac cgtgcgagtc gaacgcgctg accgacccgg
     1021 ccatgttgtc gacgtcgacc tcgacggcgg caccccagta gggcatgcct tccttgaact
     1081 cgcgacgacg ccgggtggca gtagcaccca cgtcggccac cggaacgtag aacagatcgt
     1141 ggtccgggtt gtacgacgcg tgcgtccact ccttcgcacc ggcggggccg gggtagaagt
     1201 ggcagggctc gccttccttg tccgggtact tccgcggggt caccttgccg tcgcgggtga
     1261 tgacgcccca gtcgatccgg tcgacgaacg gcgtgacgtg ctgcagctcg ccgttggtgc
     1321 ggtcaaggac gaacatgtac ccgttcttgt cgaagtgagc gagcagcttc ttgccgtccc
     1381 gctcgaacag ggtcatctcc atggtggagt cgtagtccca caggtcgtgg ggggtgaact
     1441 ggtagtgcca cttgatctcg cccgtgtcga catccagcgc gaccacgctg tcgctgtaga
     1501 ggttgtctcc cctgcggacc tcgccgtcga agtcgggagc cgggttcccg gtgcccgcgt
     1561 agtaaaggtt cagctccggg tcgtaggttc cggtgaccca gtggttcgcg cctccgcgct
     1621 gccaggcctc cccgtcggcg ggccaggtct ccgagccggg ctcacccggc ttgggcacgg
     1681 tgtaggtgcg ccagcgctgc tcaccggtct ccaggtccca gcagtcgatg tgaccgcgga
     1741 cgccgtactc gccaccggcg gaaccggtga tgagcgtgtc cttgacgacc agcggagcga
     1801 tcgaagcgct ctcgcccgcc cgcacgtcgc cgatggtctt ctgccagacc ttctggccgt
     1861 tggtggcgtc gatcgccacc aactgggcgt tctgggtcac catgtagacc ttgccgttgg
     1921 ccaccgcaca gccgcggttg acgttggcgc agcacagcgt cacgtcgaag ggaatcgcgt
     1981 gcttgtaacg ccacagctgc tcgccggtca ccgcgtcgag cgcccagagc cagccgtccc
     2041 agccggtgac gaacatgacg ccgtcgacga ccaacgggca ggcctcgaac gcgtaggtcg
     2101 aggtggaggc gatcagaccg gtcgagctcg cctggtggat cca
//
LOCUS       DATADN010000562         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_378501, whole genome shotgun sequence.
ACCESSION   DATADN010000562 DATADN010000000
VERSION     DATADN010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378501"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1280)
                     /gene="ribA"
                     /locus_tag="VHH53_07235"
     CDS             complement(81..1280)
                     /gene="ribA"
                     /locus_tag="VHH53_07235"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II RibA"
                     /protein_id="HEX2289974.1"
                     /translation="MDGVREVARVALPTQFGEFQARAFEVSCGLVYLALVKGDVVSAA
                     GVLTRVHSECLTGDVLGSLRCDCGVQLRLALRRIAIEERGLLLYVTGHEGRGVGLVNK
                     LRAYIEQDGGVDTLDANVRLGLPIDGRRYDDTAAVLHAVGVGSVRLLTNNPEKVRGLR
                     EAGITVERMEPLQTAAHHRNVGYLRTKKRRLNHVAPAGEALGTAPSAPPDAIGLLGDV
                     QPRADRPYLVVKYAQSLDGRIATITGDSKWISGEEQRSVSHALRAACDSVMVGVGTVL
                     RDDPRLTVRLVPGASPLRVVLDSTLRIPSTALVLDGDPVTVVFTTDRAAHSDRERLRA
                     LGASVRVVPGSSAGVDLPAMLRVLRELGVRTLLVEGGATMITSLLGDRLVDRLIVGTA
                     GRHRPAR"
BASE COUNT          281 a          573 c          440 g          221 t
ORIGIN      
        1 cagtgatagt cgcgatccgt ccgtccaggg actgggcgta cttcaccacc aggtaagggc
       61 ggtccgcccg cggttgtaca tcaccgagca ggccgatgac gtccggcggt gccgacgatg
      121 aggcggtcga ccagtcgatc accgagcagc gaggtgatca ttgtggcgcc gccctcgacg
      181 agcaacgtcc gtacaccgag ctctcgcagc acccgcagca tcgcgggcag gtccaccccg
      241 gctgaactgc ccggcaccac ccggacgctg gccccgagcg cccgcagccg ctcccgatcg
      301 ctgtgggcag ctcggtcggt cgtgaacacg acggtgaccg gatccccatc gagcaccagc
      361 gccgtcgacg ggattcgcag cgtggagtcg aggactaccc gcagcggcga cgcaccaggc
      421 accaggcgca cggtcagccg cgggtcgtcg cgcagcaccg tgcccacccc caccatgacc
      481 gaatcacagg ccgccctcag ggcgtgcgag acgctgcgtt gttcctcgcc actgatccat
      541 ttcgagtcac cagtgatagt cgcgatccgt ccgtccaggg actgggcgta cttcaccacc
      601 aggtaagggc ggtccgcccg cggttgtaca tcaccgagca ggccgatagc gtccggcggt
      661 gccgacggtg cagtgcccag cgcctcaccg gcgggcgcga cgtgatttaa ccgccgcttc
      721 ttggtgcgca ggtatcccac gttgcgatgg tgtgccgccg tctgcagcgg ctccatccgc
      781 tcaaccgtga tgcccgcctc gcgcagcccg cgcaccttct ccggattgtt cgtgagcaac
      841 cgcaccgacc cgacgccgac agcatgcagt acggccgcag tgtcgtcata gcgccggcca
      901 tcgatcggga gcccaagccg cacattggcg tccaaggtgt cgacaccacc atcctgctcg
      961 atgtaggccc gcagcttgtt gaccagaccc acgccgcggc cctcatgccc ggttacatac
     1021 agcagcagcc cgcgctcttc gatcgcgatc ctgcgcagcg ccaggcgaag ctggaccccg
     1081 cagtcgcacc gtaacgaacc cagcacgtcc ccagtcaggc actccgagtg cacccgggtc
     1141 agcaccccag ccgcactcac cacgtcaccc ttgaccagcg cgaggtagac caacccgcag
     1201 gaaacctcaa aggccctcgc ctgaaactcc ccgaactgcg tcggcagcgc cacccgggcc
     1261 acctcgcgca caccatccac ccaggacccc tctcccacac ctaaagcgag cgttcacgaa
     1321 taaacgaggt atgccgtgaa agaggctgcg ttctcctagt gggctgacag aggagggcag
     1381 cgatccttcc ggttcggcgc attccgacgg cctggctact actaagctga ccgcgcacct
     1441 ggacccaacc acccactgct gcattcgcct cgagcccacc gtccctcagc aacaccggac
     1501 atgaccgcat cagcg
//
LOCUS       DATADN010000563         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_209734, whole genome shotgun sequence.
ACCESSION   DATADN010000563 DATADN010000000
VERSION     DATADN010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209734"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VHH53_07240"
     CDS             <1..268
                     /locus_tag="VHH53_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289975.1"
                     /translation="TDQIVASNTTAQMITEPKQIELGGLPGFFYFYSFKDAASGQEGV
                     HSHYFLFNDKKMISLVFQALPKDHFPASAPTFDKITATFRALKK"
     gene            299..1048
                     /locus_tag="VHH53_07245"
     CDS             299..1048
                     /locus_tag="VHH53_07245"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX2289976.1"
                     /translation="MVRPPRWAGLSDRGQVRERNEDCWSADADLGLFIVSDGMGGRPA
                     GEVASEVVVAALPTLVAHHFSAEPDLAAPDAPQRLRTVLTELSAEVREGSQDTPRLAG
                     MGATAVAALVSASQATALIAHVGDSRAYLLRERSLHRLTRDHSLAQALIDAGAITDQQ
                     AAGHPARGQLTRHMGMAGEVSPDARRVALQPDDRLLLCSDGLTGMLDDAQIQAILNRS
                     SDPQHACRALTEAANDAGGIDNITVLVIAPA"
     gene            complement(1075..1785)
                     /locus_tag="VHH53_07250"
     CDS             complement(1075..1785)
                     /locus_tag="VHH53_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX2289977.1"
                     /translation="MSQPDTALVWAADGQARFERAVAECSKLRGALGGPSRLPGWTRG
                     HVVCHVARNAEGLVRLLHWARTGIETPMYPSAEARAADIEAGAGRPLDEQLDDVRNTG
                     AAFAAATAGLSAKHWEAIVQTRQGAMSAATIPWARVREVWLHLVDLNAGATIDVLPGD
                     IATALVRDVAGWMGTRVPTRIELRIAGGEAVTFGPETSAPVSVCGSAQELAGWLTGRC
                     QGDRLVASAGLPDLPSWM"
     gene            complement(1814..>2208)
                     /locus_tag="VHH53_07255"
     CDS             complement(1814..>2208)
                     /locus_tag="VHH53_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2289978.1"
                     /translation="FLTVLDEFGAYQAMIERELERFLPFLATTKVLVAAVRAGMGRET
                     AHEVIREHAVAVALAMRTNGADNDLIDRLADDSRLPLDRAALDALLTDRLSFTGVVSD
                     QIAAVVAEVQSVVARYPEAATYHTAAIL"
BASE COUNT          360 a          791 c          719 g          338 t
ORIGIN      
        1 gacggaccag atcgtggctt ccaacacgac ggcccagatg atcacagaac cgaagcagat
       61 cgagctgggc gggcttcccg gcttcttcta cttctactcg ttcaaggacg ccgcctccgg
      121 ccaggagggt gtgcactcgc actacttcct gttcaacgac aaaaagatga tcagcctcgt
      181 cttccaggcc ttgcccaagg atcatttccc tgcttccgct ccgaccttcg acaagatcac
      241 cgctacgttc cgcgctttga agaagtgacg actcacgcgt tgcaggacgc gctgtgacgt
      301 ggtgagacca ccacgctggg cgggtctcag cgaccgcggc caggtgcgcg agcgcaatga
      361 ggactgctgg agcgccgacg ccgacctcgg actgttcatc gtgtccgacg gcatgggtgg
      421 ccggcccgcg ggcgaggtgg cctccgaggt cgttgtcgcg gccctgccga cactggtggc
      481 tcaccacttc agcgccgagc cggacctcgc cgctccggac gcaccgcagc ggctgcgcac
      541 ggtactcact gaactcagcg ccgaagtgcg cgagggcagc caggacacgc cgcggctggc
      601 cgggatgggt gcgaccgctg tcgccgcgct ggtcagcgcc agccaagcca cggcgttgat
      661 cgcacacgtc ggcgacagcc gcgcctacct gctacgcgag cgcagcctgc accggctgac
      721 ccgggaccat tcgctggcgc aagcgctgat cgacgcgggc gcgatcaccg accagcaggc
      781 agccggtcac cccgctcggg gccagctcac ccgccacatg ggcatggccg gcgaggtgtc
      841 gcccgacgct cgtcgcgtcg ccctgcaacc cgacgaccgg ctcctgctct gcagcgacgg
      901 cctcaccggc atgctcgacg acgcgcagat acaagcgatc ctcaaccgca gctcggaccc
      961 tcagcacgcc tgccgcgccc ttaccgaggc ggccaacgac gcaggcggca tcgacaacat
     1021 caccgtgctc gtgatcgcgc ctgcgtgagc ggccctgcga tcagatccaa ctagtcacat
     1081 ccagctgggg agatctggca gcccagccga tgccacgagg cgatcgccct ggcagcgacc
     1141 ggtgagccaa ccggccagct cttgggccga tccgcagacc gatacggggg cgctggtctc
     1201 ggggccgaag gtgaccgcct cgccgccggc aatccgcagt tctatccggg ttggcacccg
     1261 ggttcccatc cagccggcaa catcacgcac cagcgcggtg gcgatgtccc cgggcagcac
     1321 atcgatcgtg gcgccggcgt tgagatccac caggtgcagc cacacctcac gtacccgtgc
     1381 ccaggggatg gtggcagcgg acatcgcacc ctgcctggtc tgcacgatgg cctcccagtg
     1441 cttcgcggac agccctgcgg tggccgcggc gaaggcagct ccggtgttgc ggacgtcgtc
     1501 gagctgctcg tccagtggcc gtcccgcgcc ggcttcgatg tctgccgcgc gagcctccgc
     1561 cgacgggtac atcggggtct cgatcccggt gcgtgcccag tgcagcaggc gcaccaggcc
     1621 ctcggcgtta cgggcaacgt ggcacaccac atggccgcga gtccagccgg gtagccggga
     1681 cggcccgcct agggccccac gaagcttgct gcactccgcg acggcccgtt cgaaccgcgc
     1741 ctgcccgtcg gcggcccaga ccaatgcggt gtccggctgg ctcacgtgac gtcccgctct
     1801 gtccactctc gtctcacagg atcgcggctg tgtggtaggt ggcggcttcg gggtaccggg
     1861 cgacgacgct ttgcacctcg gcgacgaccg cggcgatctg gtcggagaca accccggtga
     1921 acgataggcg atcggtgagc aatgcgtcga gagcagcgcg gtccagcggc agcctgctgt
     1981 cgtcggccag ccggtcgatc agatcgttgt cggcgccgtt ggtccgcatc gccaacgcga
     2041 ccgccacggc gtgttccctg atcacctcat gtgcggtctc ccggcccata cccgcgcgca
     2101 ccgcggcgac cagcactttg gtggtggcca ggaaagggag aaagcgttcc agttcccgtt
     2161 cgatcatcgc ctgataggcg ccgaactcat cgagcacggt gaggaagg
//
LOCUS       DATADN010000564         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_981404, whole genome shotgun sequence.
ACCESSION   DATADN010000564 DATADN010000000
VERSION     DATADN010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981404"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..799
                     /locus_tag="VHH53_07260"
     CDS             437..799
                     /locus_tag="VHH53_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2289979.1"
                     /translation="MTPDPATASPSMSVVEAAKEMIQKEKGPLPVVEGGRVVAMVTDR
                     DIIARVVAAGQDPSALRVSDIATKDLVTIGPDQDAQEARQLMAQHQLDRLLVVEEGDR
                     LVGIISEADLRRDEGPLA"
     gene            941..1150
                     /locus_tag="VHH53_07265"
     CDS             941..1150
                     /locus_tag="VHH53_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289980.1"
                     /translation="MGVDTMRLEDDDIETVWPEIHAQGGATVSDGDSGDDGDAGDSSD
                     SSDSGSSDSDADTSDSVDDSDSTDR"
     gene            complement(1424..>2580)
                     /locus_tag="VHH53_07270"
     CDS             complement(1424..>2580)
                     /locus_tag="VHH53_07270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2289981.1"
                     /translation="SDGPTSRNAAPNFLGNLAQAGLVSADLTLMSATSGVMHRAMLAV
                     RAALPHGQLLTPEVWARRHRGIVWLLWLHVAGIAAFAMVRGYGVVHGLEEASLVAMFA
                     LVAAHPAVGRRARSAAAVLGLITASAVIVHLSGGVVEAHFHFFVMIGVITLYQDWLPF
                     GLALGYVIVHHGLLGALRPTDVYNHPAAWQQPWKWALIHGGFVLAASIAYLVNWRLSE
                     SQAVEISRLVSRLQGLARSDPLTGVPNRRVWEEELPRELERARRMGTPLCVAMVDLDN
                     FKAYNDRFGHQAGDHVLKEAATLWRAQVRSTDLLARYGGEEFALLLPACALEDAIKII
                     ERLRTVTPQVTCSIGVACWDFQESAGELFERADQALYAAKAEGRNRYVAA"
BASE COUNT          417 a          856 c          933 g          374 t
ORIGIN      
        1 gcgccgcgat cggccggccc acgtgcagga gtggatcggg aacgctgcgc aggtcgacag
       61 ggccgccttc cgccggcgac gggcgccggc gcatgaccgc gatgacctgg cgattggcgg
      121 tgcccgttgg gcctcgagag gtatccacag ctggtcggtt ggtcttggct gtggccggag
      181 gtgattgcat ctggaatgtc tctggcccgt tcgatggtct ggcccacgag ccccggcgtc
      241 gtggcgtgcg ctggtgtgat gcttgtgctc cgtcaagtga ggtggtggcc ggtgatcggc
      301 cgggcctgat ccacgagttg agcacctgcc gctttcttgc gcctgcttcg gttttgaggc
      361 atctgctatg ggtagggcga cctcgtgcca agtctggttg aagggggcga gcaagatgcc
      421 cagggtacga gaggtcatga cccctgatcc ggcgacagcc tccccgtcga tgagcgtggt
      481 cgaggcggcc aaggagatga tccagaagga gaaggggccg ctgcccgtgg tcgagggcgg
      541 ccgggtcgtg gccatggtca ccgaccgcga catcatcgcc cgcgtggtcg cggccggcca
      601 ggacccgagt gcgcttcggg tgagcgatat cgccaccaag gacctggtga cgatcggacc
      661 cgaccaggac gcgcaggagg cccgccaact gatggcccag caccagctgg accgcctgct
      721 ggtcgtggag gaaggcgacc ggctggtcgg gatcatctcg gaggccgacc tgcggcggga
      781 cgagggcccg ctggcctaac ctccacgccg gcagctctgc ctagcggcgg gtgctacgcg
      841 aatggtggct cgccgctagg ccggcatggc cgcaggggtg tcgctgccgg tctccagggt
      901 agatcttggg aactggccat ctcgcagagg aggtctggct atgggcgtcg ataccatgcg
      961 gttggaggat gacgacatcg agaccgtctg gcccgagatc catgcccagg gcggtgccac
     1021 cgtcagcgac ggcgactccg gcgacgacgg cgacgctggt gacagcagcg attcctctga
     1081 ttcgggcagc agcgatagcg acgcggacac cagcgacagc gtcgacgaca gcgactccac
     1141 cgaccggtga tcagcggtca gccggccagc tgagtcgagg agcgaccggc acgggtggca
     1201 gcacagccac gagccgcacg aggacccggc ccacgagcac ggccgtgagg cccacatcca
     1261 cgaccacgga cagccaacca gctcaccggc ataaccgacg cggtcaggag ccggctaact
     1321 cggccgccta ctggcaggtt gctgtcgtgg ctgttgtggc gaccggcggg gtcgcagccg
     1381 agtcggcggg ctgctgggcg ccggcggtta tgaaggtctg gcgtcaggcc gcgacatagc
     1441 ggttgcggcc ctcggccttg gccgcgtaca gggcctggtc ggcccgctcg aacagctcac
     1501 cggcgctctc ctggaagtcc cagcaggcca ccccgatgga gcaggtcacc tggggggtga
     1561 cggtccgcag ccgctcgatg atcttgatcg cgtcctccag cgcacaggct ggcagcagca
     1621 gtgcgaactc ctcgccgccg tatcgggcca gcaggtcggt ggagcgcacc tgggcccgcc
     1681 agagggtggc ggcttccttg agcacgtggt cgccggcctg atggccgaag cggtcgttgt
     1741 aggccttgaa gttgtccagg tcgaccatgg ccacgcacag cggcgtcccc atccgccggg
     1801 cccgctccag ctcgcggggc agctcctcct cccacacccg ccggttgggg acaccggtga
     1861 gcgggtcgct gcgggccagc ccctggaggc ggctgaccaa ccggctgatc tcgaccgcct
     1921 gactttcgct gagccgccag ttgaccaggt aggcgatgct ggcggcgagc acgaacccgc
     1981 cgtggatgag cgcccacttc cacggctgct gccaggccgc cgggtggttg tagacatcgg
     2041 tcgggcgcag ggcgccgagc aggccgtggt gaacgatgac gtagccgagg gccagcccga
     2101 acggcagcca gtcctggtac agggtgatga ccccgatcat gacgaagaag tggaagtggg
     2161 cctccaccac cccgccggac aggtgcacga tcaccgccga ggcggtgatc agcccgagca
     2221 cggccgccgc cgaccgggcc cgccgaccga cggccggatg ggcggccacc agcgcgaaca
     2281 tcgccaccag cgacgcctcc tccaggccgt gaaccacccc gtagccgcgc accatggcga
     2341 aggcggcgat gccggcgacg tgcagccaca gcagccagac gatcccgcgg tgccggcggg
     2401 cccacacctc gggcgtgagc agttgcccgt gcggcagcgc cgcccggacc gcaagcatgg
     2461 cccggtgcat gacgccgctg gtcgcgctca taagagtcag atcggcagac accaggcccg
     2521 cctgagcgag gttgcctaga aagttcggag ctgcatttct cgaagtaggc ccgtcgctca
//
LOCUS       DATADN010000565         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_379061, whole genome shotgun sequence.
ACCESSION   DATADN010000565 DATADN010000000
VERSION     DATADN010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379061"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VHH53_07275"
     CDS             <1..509
                     /locus_tag="VHH53_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005445899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2289982.1"
                     /translation="ALQIDRYKTAAYTVERPLHLGAAIAGACPALVDAYGRFGADIGI
                     AFQLRDDLLGMFGDPVVTGKPAGDDLREGKRTLLLAYGLRAADRQRRTKAAKLLRGAI
                     GNPQVQHDTVTQVRELLIDLGAVAEVESRIAELTDSALASMHSTDLDEPARSRLIELA
                     AQATSRTS"
     gene            566..>1549
                     /gene="crtI"
                     /locus_tag="VHH53_07280"
     CDS             566..>1549
                     /gene="crtI"
                     /locus_tag="VHH53_07280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEX2289983.1"
                     /translation="MRTVPGPTGHVVVVGAGLAGLSAALHLLGAGRQVTLLEAQPHPG
                     GRAGRMDLAGYRLDTGPTVLTMPELVDEALAAVGDSVAARLDLVALHPAYRARFADGS
                     VINVHTDAAAMEAEVRAVAGPQEAKGYRRLRDWLTRLYQVEIGRFIGGNFDSPLDLVA
                     SRDAVRELATLAALGGFGRLGPQVGRFLGDERLQRIFSFQALYAGVPPQRALGAYAVI
                     AYMDTVAGVWFPRGGMRALPQAMADAATDAGAQLRYGTTVTALEHSGDRVTAVRTADG
                     ERFAADAVVLTPDLPVVHELLGRAPRRPVALRWSPSAVVLHAGVRPGTGSGW"
BASE COUNT          233 a          527 c          529 g          260 t
ORIGIN      
        1 ccgcactaca gatcgaccgg tacaagacgg ccgcctatac cgtcgagcgt cctctgcacc
       61 taggggcggc aatcgccgga gcatgccccg cgttagtgga cgcctacggc cgtttcggcg
      121 ccgatatcgg catcgccttc cagctccgcg acgacttgct cggcatgttc ggcgaccctg
      181 tggtcaccgg caagcccgcc ggcgacgacc tccgcgaggg caaaaggacg ttgctgctag
      241 cctacggtct gcgcgccgct gaccggcaac gccgcactaa ggcagccaag ctgctgcgcg
      301 gcgcgatcgg aaatccgcag gtgcagcacg acaccgtaac tcaagtacgg gagctactga
      361 tcgatctcgg cgcagtcgct gaggtggaaa gccggatcgc tgagctcacc gactccgctc
      421 tggccagcat gcactccacc gacctggacg agccggcccg ctcccggttg atcgagttag
      481 cggcgcaggc aacttctcga acctcgtgag tggtattgag tgcggcaaca ctgttacgca
      541 ctcacgacca cgctagggtc cgtgcgtgcg tactgtgccc ggacccaccg gccacgtagt
      601 agtcgtcggt gctgggctgg ccgggctttc cgcggccctg cacctgctcg gagccggacg
      661 gcaggtgacc ctgctcgaag cgcagccgca tcccggcggt cgggccggcc ggatggatct
      721 ggcgggctat cgcctcgata ccggccccac cgtgctcacc atgcccgagc tggtcgatga
      781 ggcgctggcc gcggtgggtg attcggtcgc cgcacggctg gacctggtgg cgttacaccc
      841 cgcctaccgg gcccgcttcg ccgacggttc ggtgatcaac gtgcacaccg atgccgctgc
      901 gatggaagcc gaggtcaggg cggtggccgg accgcaggaa gcaaagggct accggcggtt
      961 gcgggactgg ctgaccaggc tctaccaggt cgagatcggc cggttcatcg gcggcaactt
     1021 cgactccccg ttggacttgg tggccagccg tgacgcggtc cgcgagttag ccacgctggc
     1081 cgcgctgggc ggcttcggca ggcttggccc ccaggtcggc cggttcctgg gcgacgagcg
     1141 gctacaacga atcttctcct tccaggcgct ctacgccggg gtacctccgc agcgtgccct
     1201 cggtgcctac gcggtgatcg cgtacatgga caccgtggcc ggggtctggt ttccccgagg
     1261 cggaatgcga gcgttgcctc aggcgatggc cgacgctgcc accgacgccg gcgcccagct
     1321 tcgctatggc accacagtga ccgctctaga gcactccggg gaccgcgtca cggcagtgcg
     1381 taccgcagat ggcgagcggt ttgccgccga tgcagtggtg ctcactcccg acctaccggt
     1441 ggtccatgag ttgcttggca gggctccacg gcggccggtg gcactgcgct ggtcgccgtc
     1501 ggcggtggta ttgcatgccg gagtccggcc gggtaccgga tctggctgg
//
LOCUS       DATADN010000566         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_981615, whole genome shotgun sequence.
ACCESSION   DATADN010000566 DATADN010000000
VERSION     DATADN010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981615"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="VHH53_07285"
     CDS             complement(<1..1030)
                     /locus_tag="VHH53_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2289984.1"
                     /translation="MTKRQDEHPDQRSSSDEATPGGSTAPAPPAGIRTIFRWFWPATR
                     PYRGRLLLSLLLVAFVPFLEAAQIWMFKLLIDDVLAPRDFHAFPPIAAGYLGIGLALG
                     LVSFVDEWLSSWLGQRFVLDLRTRLFSHLHKLSVDFFDRRQLGDTLSRLTGDIDAIEQ
                     LVLSGVTRTLSYAFQILLFTGALFYLNWHLGLAALIAAPAFLMAARHFSLRIKNASRE
                     QRRRSGAVTAAAEESLGNIALVQAYGRQAAEVDRFHEQNVGRFTAAMAATRLRALFQP
                     LTELLEIIGIVLVMGLGVWELQQGRITLGGLLVFVTYLTRLYSPIRGAGRLSNTIYAA
                     SASAERIIE"
     gene            complement(1330..>1563)
                     /locus_tag="VHH53_07290"
     CDS             complement(1330..>1563)
                     /locus_tag="VHH53_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289985.1"
                     /translation="HSWAKDTISVADIREIGGLPANTAVIEENLRDGTERTLGEEETA
                     ASGQTLGRARCRPSGSTSAQLDRVRWGPSCYPT"
BASE COUNT          274 a          494 c          530 g          265 t
ORIGIN      
        1 gctcgatgat gcgttcggcg ctcgcgctcg ctgcgtagat cgtgttggac aggcggccgg
       61 ccccccggat cgggctgtag agcctggtga ggtaggtcac gaagaccagt aggccgccca
      121 gggtgatccg accctgctgc agctcccaca ctcccagtcc catgaccagc acgatcccga
      181 tgatctccaa tagctcagtc agtggttgaa acagggctcg cagccgggtc gcggccatcg
      241 ccgcggtgaa ccggccgacg ttttgctcat ggaaccggtc gacctcggcg gcttggcggc
      301 cgtaggcctg caccagagcg atgtttccca ggctctcctc ggcggccgcg gtgacagccc
      361 ccgatcgccg tcgctgttcc cgggaggcgt tcttgatgcg gagggagaag tgccgcgcgg
      421 ccatcaagaa agcaggggcg gcgatcaacg cggccagtcc caggtgccag ttcaggtaga
      481 acaacgcgcc ggtgaacagc aggatctgga atgcatagga cagggttcgg gtgacaccgg
      541 agagcacgag ctgctcgatg gcgtcgatgt cgccggtcag ccgcgacagg gtgtcgccga
      601 gctggcggcg gtcgaagaag tccaccgaca gcttgtgcag gtggctgaac aggcgagtgc
      661 gcaggtcgag cacgaaccgc tgaccaagcc aggacgacag ccactcgtcg acgaaggaga
      721 cgagtcccag ggctaggccg atcccgaggt aaccagctgc gatcgggggg aatgcgtgga
      781 agtctcgggg cgccagcacg tcgtcgatca ggagcttgaa catccagatc tgcgccgctt
      841 ccaggaacgg cacgaaggcg accagcagca ggctgagcag caggcggccc cggtacgggc
      901 gggtcgctgg ccagaaccag cggaagatgg tccggatgcc agccggcgga gcgggcgccg
      961 tcgacccacc gggcgttgcc tcgtctgacg aagaacgctg atccgggtgc tcatcctgtc
     1021 gcttggtcaa gcccagcccc ccgttacacg gcagaggccc caccccagcg aacgaagtga
     1081 ggccccggcc ctggcgtcgt gccgttgctg cttagtggcg acggcgacga cgacggctgt
     1141 ggctgctgct gtggctgcaa ctatggctgc ggctgcggcg gtgactgacg ctgcgcgccc
     1201 ggtcgaaggc ctcgaagtaa cggctcacgg cttccccttc cgttcatctt cagtgatggc
     1261 gctgttcagc catcggtaag atttacgtcg ccgggaaggc cttcgacgtt acagagcatc
     1321 accgcttagt tacgttgggt agcagctcgg accccatcga actcgatcaa gctgggctga
     1381 agttgatccg cttggtcggc atcttgccct ccccagagtt tgtccggatg cagcggtctc
     1441 ctcctcgccc agcgtccgtt cggtgccgtc gcgcaggttc tcctcgatga cggccgtgtt
     1501 cgcgggcagg cccccgatct cccgaatgtc agcgaccgag atggtgtcct tggcccatga
     1561 atg
//
LOCUS       DATADN010000567         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1132119, whole genome shotgun sequence.
ACCESSION   DATADN010000567 DATADN010000000
VERSION     DATADN010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132119"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VHH53_07295"
     CDS             <1..740
                     /locus_tag="VHH53_07295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2289986.1"
                     /translation="SYRGRAEMLRPFTTPNTWLDVGTGYGHFCQAAREVWPGTIFDGL
                     DQSLGVEEAQRRGWIDQGYRGEFVALADELAGRYDVVSMHHYLEHTREPFDELDAVAK
                     ILPAGSYILIELPNPESFLGRLLGRWWMPWFQPQHQHMIPLGNLVQALSDRGFSAVAV
                     DRGAAHQGNDLLCGLALALIAVAPDPRLPWLTTEPTRGRRIRHAAVWAAGRPLLPVAY
                     LLDMLLYQVVRRAQGGNAYRVLARKDG"
     gene            742..1503
                     /locus_tag="VHH53_07300"
     CDS             742..1503
                     /locus_tag="VHH53_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2289987.1"
                     /translation="MTTPEDFAGRVALVTGAASGIGAAVATRLAADGAYLTVADNDYL
                     GAECTVRRIREAGGRAEPVAVDVTDPASVEAAVKATVAAFGGLHLAVNSAGVTGPAVP
                     TGEYDPQDWRRVIDVDLTGVFYCLRYEIPALLIAGGGSIVNMSSILGSNGFPQQAAYT
                     AAKHGVIGLTKSAALEYASQGIRINAVAPGFVETPLVTKGLTPLRRRTLVRAHPIGRL
                     ADASEIAELVAFLLSDHASFITGSCNLVDGGYAAH"
     gene            complement(1438..1710)
                     /locus_tag="VHH53_07305"
                     /pseudo
     CDS             complement(1438..1710)
                     /locus_tag="VHH53_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          291 a          627 c          606 g          322 t
ORIGIN      
        1 ggtcctaccg ggggcgggcc gagatgctca ggccattcac cacgcccaac acgtggctgg
       61 acgtcggtac cgggtacggc cacttttgcc aggccgcgcg agaggtttgg ccgggcacca
      121 tcttcgacgg gctggaccag agcttgggcg tcgaggaggc gcagcgccgt ggctggatcg
      181 accaaggcta ccggggggag ttcgttgcgc tcgccgacga gctggcaggc cggtacgacg
      241 tggtcagcat gcaccattac ctggagcaca cgcgggagcc gttcgacgag ctggatgctg
      301 tggccaagat tctcccagcc ggcagctata tactcatcga gctgcccaat cccgagtcct
      361 tcttgggccg cctgctcggc cgctggtgga tgccgtggtt ccagccgcag catcaacaca
      421 tgatcccgct gggtaatctg gtacaggcgc tgtctgaccg tgggttctct gcggtggccg
      481 tcgatcgcgg ggcggcccac cagggcaacg acctgctctg cggtcttgcg ctcgcactca
      541 tcgccgtcgc tcctgacccc aggctcccgt ggctgaccac cgagccgacc cgcgggcgac
      601 ggattcggca cgccgcagtg tgggccgctg ggcgaccgct attgccggtt gcgtacctgc
      661 tggacatgtt gctctatcag gttgtgcgcc gcgcacaagg aggcaacgcc tatcgggtgc
      721 tggcacgcaa ggacggctga ggtgaccaca ccggaagact tcgccggtcg agttgccctg
      781 gtcaccggcg ctgcctccgg gatcggggcc gccgtcgcga cccgcctcgc cgctgacggc
      841 gcctacctca cggtggccga caatgactat ctcggcgccg agtgcacggt acgccggatc
      901 cgtgaagccg gcggccgcgc cgaacccgtc gcggtcgacg tcaccgaccc agcctccgtc
      961 gaagctgcgg tcaaggccac cgtcgccgca ttcggcggcc tgcacctcgc ggtgaacagt
     1021 gccggagtga ccggccccgc cgtgcccacc ggagaatacg acccgcagga ctggcggcgc
     1081 gtgatcgatg tcgatctcac cggtgtcttc tactgcctgc gctacgagat tcccgctctg
     1141 ctcatcgccg gcggtggatc gatcgtgaac atgtcatcca ttctcgggag taacggattt
     1201 ccgcagcagg ccgcctatac ggcagccaag cacggcgtta tcggcctcac caagagcgcc
     1261 gcactcgagt acgcctccca aggcatccgc atcaacgcgg tcgctcccgg cttcgtcgaa
     1321 acaccactcg tgaccaaggg tctcactccg ctccgccgcc ggacgttggt ccgtgcccac
     1381 ccgatcggcc gactcgctga cgcgagtgag attgccgagc tggtcgcgtt cctgctctca
     1441 gaccatgcct cgttcatcac cggcagctgc aacctggtcg acggcggcta cgcggcacat
     1501 tgagttcacg ggtgatgcac gtcgtatcgt ggtgaggggc caagcgtcaa gtccgcggat
     1561 cgcctcgcgg gcgcgggcgg ctcgcaggcc cggcgtcagc tcgatattgc ataggtgccg
     1621 gaagccaaac cgctcgtagt aaggcatgtt ccatggcacg tcggtaaacg tggtcagggt
     1681 caacgccgcc aggccgtgac gctgcgccca cgcgtgcatc ggtagttctc ggaacggcgc
     1741 gccggcggcc tgttccaggg tctgcaggag gggaatcgtc gctgcgagcg gcacggatca
     1801 tggtgtctcc tgctgcgccc ggtatcgaag tgagcattct ctccac
//
LOCUS       DATADN010000568         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1132192, whole genome shotgun sequence.
ACCESSION   DATADN010000568 DATADN010000000
VERSION     DATADN010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132192"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VHH53_07310"
     CDS             <1..655
                     /locus_tag="VHH53_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEX2289988.1"
                     /translation="LIALGRRYRDTPTELLLSRVMAVVFAAFLVPVEVYWLLPGQAGM
                     IHSLPLQLCDLAAMATLWALWTHSPTAFALTYFWGLTLTSQAFLSPELTGPDFPSLQF
                     LSFFGMHSLVLWAAIYLTWGAGLRPDWRSYRITLLITVGWAVVMFAFNHVAGTNYGFL
                     NAKPTAASLLDLFGPWPWYLVTALALGATFWALITYPWTRRTHPAGSAGLRTAKLPR"
     gene            complement(685..>2009)
                     /gene="malQ"
                     /locus_tag="VHH53_07315"
     CDS             complement(685..>2009)
                     /gene="malQ"
                     /locus_tag="VHH53_07315"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX2289989.1"
                     /translation="IDAYRRADPALRVEVDTLRLEPEGERIDYDAVWRAKRAALELLW
                     RAEGIPCPVMSDGLRDFTTFCALAERHGPRWSRWPSELRHPATASADPQRVAFHAWVQ
                     QQCTEQLAATRDAARGMAIGVVHDLAVGVDPEGADAWALQDVLANGVTVGAPPDIFNQ
                     QGQDWGLPPWRPDRLAATGYAAYRDMLRAVLAHADGLRIDHVAGLWRVWWVPPGASPA
                     QGTYVHYDATAMLAALALEAHRAGAVVVGEDLGTVEPEVTEALAAQRMLGCTVLWFAR
                     DEDAPGQPLLAPRQWPQRAAASISTHDLPTAVGFLRGEHVRARAALGLLNDEAAEWAQ
                     ARAERAELVGLLRGEGLLEDGEAEDDIVVAMHALLARTPCQLLLVSPYDVVGEVRQPN
                     LPGTIDEYPNWRLPLSVTLEELQRDPRVRRAAEVLTAGVDGTNTPLRT"
BASE COUNT          292 a          716 c          653 g          348 t
ORIGIN      
        1 gctcatcgcc ctgggtcggc ggtaccgcga cacgccgact gagctgctgt tgagccgggt
       61 gatggcggtg gtctttgctg cgttcctggt gccggtcgag gtctactggc tattgcccgg
      121 tcaagcaggg atgatccatt cgctgccgct gcaactgtgt gatttggcgg cgatggcgac
      181 gttgtgggcg ctgtggacgc attccccgac ggcctttgcg ctgacctact tctggggcct
      241 gacgctgacc tcgcaggcgt ttctcagtcc agaactcact ggcccagact tcccgtcgct
      301 gcagttcctg tcgtttttcg gcatgcacag cctggtgctc tgggccgcga tctacctcac
      361 ctggggggcc gggttgcgcc cggactggcg cagttaccgg atcacgctgc tcatcacggt
      421 cggctgggcc gtcgtgatgt tcgccttcaa ccacgtcgcc ggaaccaact acggctttct
      481 caacgccaag ccgaccgcgg catccctgct ggacctgttc ggcccctggc cctggtatct
      541 cgtcaccgcc ctcgcactcg gcgccacctt ctgggcgctc atcacctacc cctggacacg
      601 gcgaacgcat ccagcgggct cagcggggtt gcggacggcc aaattacccc gctgagcccg
      661 ctgaatgcat cggcggctcg cgggtcaggt tcgtagcggc gtattggtgc cgtccacccc
      721 cgcggtcaag acttcggctg ctcgccggac ccgtggatca cgctgcaact cttccaaggt
      781 caccgatagc ggcagccgcc agttcgggta ctcgtcgatg gtgccgggca ggttgggttg
      841 gcggacctcg cccaccacgt cgtagggcga aaccagcagc aactggcacg gtgtgcgagc
      901 cagcagtgcg tgcatcgcca ccacgatgtc gtcctcggcc tcgccgtcct cgagcaggcc
      961 ctcgccacgc aagagaccga cgagctccgc acgttcagca cgcgcttggg cccactccgc
     1021 ggcttcgtcg ttgagcagtc ccagtgccgc gcgcgcccgc acatgttccc cgcgcagaaa
     1081 gccgaccgcg gtgggcaggt cgtgggtgga gatactggct gcagcgcgtt gcggccattg
     1141 ccgcggagca agcaacggct ggccgggggc atcctcgtcg cgggcgaacc acagcaccgt
     1201 gcagcccagc atccgctgcg ccgcgagcgc ctccgtgact tcgggttcca ccgtgccgag
     1261 gtcctcaccc accactaccg cgccggcgcg gtgggcctcc agcgccagcg cggcgagcat
     1321 cgccgtggcg tcgtaatgta cgtaggttcc ctgggcgggg gatgcccccg gcggcaccca
     1381 ccacacccgc cacagaccgg caacatgatc aatgcgcaac ccatcggcgt gggcgagcac
     1441 ggcgcgcaac atgtcccgat aggcggcgta cccggtggca gccagccgat ccggacgcca
     1501 cggtggcaac ccccagtcct ggccctgctg attgaagata tccggcgggg caccgacggt
     1561 caccccgttg gccagtacgt cctgcagcgc ccaggcgtcg gcaccttcgg ggtctacccc
     1621 gacggcaagg tcgtgcacca ccccgatggc cattccgcgg gcagcgtcgc gggtggcggc
     1681 gagttgctcg gtgcactgct gctgcaccca ggcgtgaaat gccactcgtt gcgggtccgc
     1741 gctcgccgtc gccgggtgcc gcagttcgct gggccagcgt gaccagcgtg ggccgtgccg
     1801 ctcagcgagt gcgcagaagg tggtgaagtc acgcagcccg tccgacatca ctgggcacgg
     1861 gattccctcg gcccgccaga gcagctccaa cgcggcccgc tttgcccgcc agacggcgtc
     1921 atagtcgatc cgctctcctt ccggctccaa ccgaagggtg tccacctcga ctcgtaatgc
     1981 cggatccgcg cggcggtagg cgtcgatgt
//
LOCUS       DATADN010000569         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1132256, whole genome shotgun sequence.
ACCESSION   DATADN010000569 DATADN010000000
VERSION     DATADN010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132256"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VHH53_07320"
     CDS             complement(<1..874)
                     /locus_tag="VHH53_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX2289990.1"
                     /translation="MTDAFTTSPDSTDTVHETTAILDNGTFGTRTVRFETGRLARQAA
                     GSVVAYLDDETMLLSATTVSKQPKDQFDFFPLTVDVEERMYAAGRIPGSFFRREGRPS
                     TDAILTCRLIDRPLRPTFVDGLRNEVQVVVTVLSLNPADPYDVLAINAASASTQLSGL
                     PFSGPVGGTRISLIDGTWVAFPTHEQIRRSVFDMVVAGRLVSDGPHSDVAIMMVEAEA
                     TEATIELVAGGAQAPTEEIVAAGLEAAKPFIRTLCQAQQELAQAVGKPVLEFPTFLAY
                     ESDVMAAVRDAVGDE"
     gene            complement(1102..1371)
                     /gene="rpsO"
                     /locus_tag="VHH53_07325"
     CDS             complement(1102..1371)
                     /gene="rpsO"
                     /locus_tag="VHH53_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX2289991.1"
                     /translation="MALSTEQKSEILTEYGLRGGDTGSPEAQVALLTKRITDLTEHLK
                     QHKHDHHSRRGLLLMVGRRRNLLNYLAKVDIGRYRSLIERLGLRR"
     gene            complement(1612..2100)
                     /locus_tag="VHH53_07330"
     CDS             complement(1612..2100)
                     /locus_tag="VHH53_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2289992.1"
                     /translation="MEEQLTVTRNVALQREWYGEPLGDRVRRLVVAFAVSQAQLAEVL
                     GLSPPMLSQLMSGRRAKIGNPAVLARMVMLERRVLTPEVASGDPAAIRAALQEVRDSR
                     PTVSCDSLPVEEDSDEVLVPGLRRLAGPAELAAAASLLQERFPLLAGLLRRASGADPA
                     AD"
BASE COUNT          403 a          760 c          773 g          376 t
ORIGIN      
        1 gctcatcgcc caccgcatcg cggaccgctg ccatgacgtc agattcatag gcgaggaagg
       61 tggggaattc cagcaccggc ttaccgaccg cctgtgccag ttcctgctgg gcctggcaca
      121 gcgtgcgaat gaacggcttg gcggcctcca gcccggcagc cacgatctcc tcggtcggcg
      181 cctgcgcgcc cccggccact aactcgatgg tggcctcggt agcctcggct tcgaccatca
      241 tgatcgccac atcgctgtgg gggccgtcgc tgaccagccg tccggcgacg accatgtcga
      301 ataccgaccg cctgatctgc tcatgggtcg ggaaggccac ccaggtgcca tcgatgaggg
      361 agatcctggt accaccgacc ggacccgaga atggcaagcc ggacagctgg gtggacgctg
      421 acgcggcgtt gatggcaagg acgtcatagg ggtcggccgg gttgaggctc aggaccgtga
      481 cgacgacctg gacctcgttg cgtaagccgt ccacaaacgt tgggcgcagc ggccggtcga
      541 tcagccggca agtgaggatc gcatcggtcg acgggcggcc ttcgcggcgg aagaaggagc
      601 cgggaatacg tccagccgca tacatccgct cctcgacgtc aacggtcaag ggaaagaaat
      661 cgaactggtc cttgggttgc ttggacactg tggtggcgga cagcagcatg gtctcgtcgt
      721 cgagatacgc gacgacggat ccagcggctt ggcgggccaa ccgaccagtc tcgaaacgca
      781 cggtgcgggt accgaaggtg ccgttgtcca ggatggcggt ggtctcatgc accgtgtctg
      841 tggagtctgg ggaagtggta aaggcgtcgg tcaattcatc tcctcctcgc aacgcggacc
      901 gcggaggcac caccggtacg ggagcgagga cgaggccggt cttcgatcga agcacccggg
      961 aacatcacag tgctcccgga agccactacc gaggaccggc gaacgacgct cccgaggacg
     1021 ggtggcgagg cggctcgtgc ggcgatgtgt gattgtcaat tcagcagcct gagccaacca
     1081 tcctgggtcg gccagcctgg gtcaacggcg caggccgagg cgctcgatca gcgaccgata
     1141 gcgaccgatg tcgaccttgg cgaggtagtt caacaggttg cggcggcggc cgaccatcag
     1201 gagcaggccg cgtcgggagt ggtgatcatg cttatgctgc ttgaggtgct cggtgaggtc
     1261 ggtgatccgc ttggtcagca gggccacctg cgcctcaggg gaaccggtgt ctcccccccg
     1321 cagcccgtac tcggtcagga tctcgctctt ttgctcggtg gacagcgcca cgggtcgatc
     1381 ttctcctgtc atctgcgtac cgtgcgggcc ccggggatcg ccctgcgctg tgcctgacgg
     1441 accgggcctg atggaccggg gcgtggccgg gcaaagcgaa gccgggggca gtcatctgcc
     1501 gacggcgccg accgccacgg accgcagccg gacccgtgct gaggttacca gttactcatc
     1561 agcgcccggc atcgccctgg atcgccggca tcgcccagat cgctgtatga gctagtcagc
     1621 ggccgggtcg gcaccagaag cgcgccggag taacccggcg agcagcggga acctctcctg
     1681 cagcaggctg gcggcggcag ccagctcggc gggaccggcc aacctgcgca gccccggaac
     1741 aaggacttcg tcactgtctt cctcgaccgg cagcgagtca cagctgacgg taggccggga
     1801 gtcgcgcacc tcctgcagag ctgcacggat cgcggccggg tcaccggatg cgacctcagg
     1861 ggtgagcact cggcgctcca acatcaccat tcgagccagc accgcgggat tgccgatctt
     1921 tgcgcggcgc ccactcatca gctggctgag catcggcggg ctcagcccca gcacctcggc
     1981 cagctgcgcc tgggaaacgg cgaaggcgac cacgagtcgc cgcacccgat cgccaagcgg
     2041 ctccccgtac cattctcgct gcagcgcaac gttgcgggtg acggtgagct gctcctccac
     2101 cggcctcccc ttccctgcgc tgtaccagta ccagccccac tatcaccgtc gcgaattacc
     2161 ggcatcttgc caggccgcag tcgtttgagg gcggtgatcg gcgagttgct cacctatcga
     2221 ggtcaagcac ctcccgggtt cgtgccacat cgtcttcgat ctgggacacc agcttgtcgg
     2281 tggtgtcgaa gcgccacatg ccacgcagcc ga
//
LOCUS       DATADN010000570         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_525818, whole genome shotgun sequence.
ACCESSION   DATADN010000570 DATADN010000000
VERSION     DATADN010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525818"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..552
                     /locus_tag="VHH53_07335"
     CDS             4..552
                     /locus_tag="VHH53_07335"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2289993.1"
                     /translation="MLYLTEAVDMVLGGALDDDGIRPRQFDGLDGVLWAPLLHGDWPH
                     LVANTVPVLVLGFLVLAGGIGQFIVVTATIWLVAGLGTWLAGSPGVHLGASVLIFGWL
                     VFLLIRGFFARSLLQILLAVVLFGLWGGVLWGVLPGAVGVSWEGHLFGAVGGLLAARL
                     VVRGPRPRRERPGLDRPGVRSS"
     gene            549..1340
                     /gene="murI"
                     /locus_tag="VHH53_07340"
     CDS             549..1340
                     /gene="murI"
                     /locus_tag="VHH53_07340"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX2289994.1"
                     /translation="MSAPIGVFDSGVGGLTVARAIADQLPGERLRYVGDTAHGPYGSL
                     PIAQVRSRALAVADELVGSGVKLLVIACNTASAACLADARERYPIPVVEVVRPAVRRA
                     AAATHSGLVGVIGTRATIRSRAYPDAFAAAPHVDVIGVACPRFVDFVERGMTSGRQVL
                     GLAQSYLEPLQQAGVDTLVLGCTHYPLLTGVLQIVMGDRVTLVSSAEETAKDVLRVLT
                     DAELLRTEPDEPAHAFTATGDPTSFARLARRFLGPLLMAAAPAVG"
     gene            1359..>1831
                     /locus_tag="VHH53_07345"
     CDS             1359..>1831
                     /locus_tag="VHH53_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2289995.1"
                     /translation="MLLTVLGCAGSVPGPESPGSGYLVRAGNTAVVLDLGNGTLGALQ
                     RYLDPFRLDAVILSHLHPDHCADVSAMTVYRRYHLHPPPRCDVLPLYGPTDVDERLVA
                     AYAPSAAERDVTDLSDIYDFHPVCDPFRVGPLEVRAAPVEHPCEAYALRISHAGST"
BASE COUNT          210 a          633 c          644 g          344 t
ORIGIN      
        1 ggggtgctgt atctcactga agcggtggac atggttctcg gcggtgccct cgacgacgac
       61 gggatccgac cccgtcagtt cgacgggctg gacggcgtcc tgtgggcccc gctgctgcac
      121 ggcgactggc cccatctggt cgccaacacg gtgccggttc tagtgctcgg gttcctcgtg
      181 ctggccggcg gcatcggcca gttcatcgtg gtgaccgcca cgatctggct ggtcgccggc
      241 ctgggtacct ggctcgccgg aagccccggc gttcatctcg gtgcctcagt tctgatcttc
      301 ggctggctgg tcttcctact catccgcggc ttcttcgccc gcagcctgct gcagatcctg
      361 cttgcggtgg tgttgttcgg gctttggggc ggcgtgctgt ggggcgtgct gccaggcgcc
      421 gtcggggtct cgtgggaggg gcacctgttc ggagcggtgg gtgggctgct cgcggcccgg
      481 ctggtcgtcc gcggccctcg gccccgccgg gagcgaccgg gcttggatcg gcccggggtg
      541 agaagctcgt gagcgccccg atcggggttt tcgactccgg tgtcggcggg ctcactgtcg
      601 cacgtgccat cgccgaccag ctaccgggcg aacggctgcg ctacgtcggc gataccgcgc
      661 acggcccgta cggctcactg cccatcgcgc aggtccgctc ccgcgcgctg gcggtagccg
      721 atgagctggt gggcagcgga gtgaagctgc tggtcatcgc ctgcaacacg gcgtcggcgg
      781 cctgcctagc cgatgcccgg gagcgttacc ccatcccggt ggtggaggtg gtgcgtcccg
      841 cggtgcggcg ggccgccgcc gccacccatt ccggtctggt gggagtgatc ggaactcggg
      901 ccaccatccg gtcccgagcg tatcccgacg ccttcgccgc tgccccgcac gtcgacgtga
      961 tcggggtggc ctgcccgcgg ttcgttgact tcgtcgagcg cggcatgacg tcgggacgcc
     1021 aagtcctcgg cctggcgcag tcgtatctcg agccacttca gcaggccggc gtggacaccc
     1081 tcgttctggg ctgcacgcac tacccgctgc tcaccggcgt gctgcagatc gtcatgggtg
     1141 atcgggtgac cctggtgtcc agcgcggagg aaaccgccaa ggacgtgttg cgggtgctta
     1201 ccgatgccga gctgttgcgc accgagcccg atgagcctgc gcacgcgttc accgcaaccg
     1261 gggatccgac gtccttcgct cggctggctc gccgcttcct cggcccgttg ctcatggcgg
     1321 cagcgcccgc ggtgggttga gctggaagga gaatgaccat gctgctcacg gtgctgggct
     1381 gcgccggtag tgtcccgggt ccggagtccc ccggttccgg ttatctggtc cgagccggga
     1441 ataccgccgt ggtgctcgac ctgggcaacg ggacgttggg cgcgctgcag cgttatctcg
     1501 accccttccg tctcgacgcg gtgatcctgt cccacctgca ccccgatcat tgcgctgacg
     1561 tgtctgcgat gaccgtgtac cgccggtacc acctgcaccc accgccgcgg tgtgacgtgc
     1621 tgccgctgta cgggccgacc gacgtcgacg agcgcctggt tgccgcttac gcccccagcg
     1681 ccgcggagcg cgacgtgacc gacctgtccg acatctatga cttccacccg gtctgcgacc
     1741 cgttccgggt cggtccactc gaggtgcgcg ccgcgccggt cgagcatccc tgcgaggctt
     1801 acgcgttgcg gatcagccac gccgggtcga c
//
LOCUS       DATADN010000571         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_812661, whole genome shotgun sequence.
ACCESSION   DATADN010000571 DATADN010000000
VERSION     DATADN010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812661"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1041)
                     /gene="dhaK"
                     /locus_tag="VHH53_07350"
     CDS             complement(49..1041)
                     /gene="dhaK"
                     /locus_tag="VHH53_07350"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEX2289996.1"
                     /translation="MKMLINVPETVVADALRGLAAAHPELTVDVENRVIVRRDAPVAG
                     KVGLVSGGGSGHEPLHGGFVGPGMLSAACPGEVFTSPVPDQMLRAAAAVDSGAGVLFI
                     VKNYTGDVLNFDMAAELAEDEGIQVAKVLVNDDVAVTDSLYTAGRRGTGATLFVEKIA
                     GAAADEGMPLERVEAIARQVNENSRSFGVALSACSTPAKGSPTFDLPPGELELGIGIH
                     GEPGRERRAMMTSGEIAEAAVEAVVEDLRPRNPVLVLVNGMGATPLLELYGFNAEVHR
                     VLAARGVPVARVLVGNYVTSLDMAGASLTLCQVDEEMLRLWDAPVKTPGLRWGV"
     gene            1355..1536
                     /locus_tag="VHH53_07355"
                     /pseudo
     CDS             1355..1536
                     /locus_tag="VHH53_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612357.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          231 a          531 c          512 g          262 t
ORIGIN      
        1 ggatctcctt gcctggtagg tacgtggtgg tcctctaccc ctttcgcgtc acacgcccca
       61 gcgcagaccc ggcgtcttca ccggggcgtc ccacagccgc agcatctcct cgtcgacctg
      121 gcacagggtg agggaggcgc ccgccatgtc cagggaggtg acgtagttgc ccacgagaac
      181 gcgggcgacg gggacaccgc gggcggcgag cacgcggtgc acctcggcgt tgaagccgta
      241 cagctcgagc aggggcgtcg cgcccatgcc gttgacgagg accagcaccg gattgcgcgg
      301 cctcaggtcc tccaccaccg cctcgacggc ggcctcggcg atctcgccgg acgtcatcat
      361 cgcccgccgc tcccggccgg gttcgccgtg gatgccgatg cccaactcca gctcgccggg
      421 cggcagatcg aaggtggggc tgcccttggc cggtgtgctg cacgcgctca gcgcgacacc
      481 gaaactgcgg gagttctcgt tgacctgccg ggcgatggcc tccacccgct ccagcggcat
      541 gccctcgtcg gcggcggcac ccgcgatctt ctccacgaac agggtcgcgc cggtaccgcg
      601 ccgccccgcc gtgtaaagac tgtcggtcac cgccacgtcg tcgttgacca gcaccttcgc
      661 gacctgaatg ccctcgtcct cggccagttc ggcggccatg tcgaagttga gcacgtcacc
      721 ggtgtagttc ttcacgatga acagcacccc ggccccgctg tccacggcgg cggccgcgcg
      781 cagcatctgg tcgggtaccg gggaggtgaa cacctcgccg ggacaggccg ccgacagcat
      841 gccgggacct acgaaacccc cgtgcagcgg ctcgtgcccc gagccaccgc ccgagaccag
      901 ccccaccttc ccggccacgg gagcgtcccg ccgcacgatc acccggttct ccacgtccac
      961 ggtcagctcc gggtgggcag ccgccagacc gcgcagcgcg tccgcgacca cggtctccgg
     1021 gacgttgatc agcatcttca tgggtacctc ctggtgagac tagcagatga gcctctgacc
     1081 ttcattcttg caggtcagga tgtgtcaggc cagttttcga tcttggcggt tcctggtggt
     1141 ccggggcggg ctgtggcggt actgatgctg actcaatgct gacttgatga cggggcgtca
     1201 gttcccctgt tgtggtcggt ggcggtcgat cggagcgctg ctgccggaaa gtatcaacct
     1261 tgcggcagcg aacgtcacgg gcacagcgca cctagggact gtctttagtt gtgatcaagc
     1321 ccaaggtcgg gtgccagtcc ggtaggactg tgttgtgacg cgtgcgcaac tcaccgagag
     1381 cgaatgggag ttcatcgggc cgttcctacc ggtcggcagg ttcggcccgt accccgagcg
     1441 gtctgcgcga gcagttcgag ggggtgatct ggcggttccg caccggcagc cagtggcggg
     1501 aagtgcccga gcggttcggc gcctggcaga ccatct
//
LOCUS       DATADN010000572         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1263541, whole genome shotgun sequence.
ACCESSION   DATADN010000572 DATADN010000000
VERSION     DATADN010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263541"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VHH53_07360"
     CDS             complement(<1..493)
                     /locus_tag="VHH53_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX2289997.1"
                     /translation="MTAHGGSRLGRVVPLAGLAGRTAGEAVVAALRKRLTGEDSAEFH
                     ERTSERYAAMLGHSKGVLMKAGQVLSFVALGKLVPPERQRVYQAAMARLQADAPPMAP
                     ELAAAVVEAELGAPPEEVFAEFDPQAFAAASIGQVHAARLADGRQVAVKVQYPGVDQA
                     IRAD"
     gene            545..1174
                     /locus_tag="VHH53_07365"
     CDS             545..1174
                     /locus_tag="VHH53_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2289998.1"
                     /translation="MSDLSTVATRDRIIDAAMELFSEHGYRGTSITRIEQAAGLSPGA
                     GGIYHHFPSKEALLTAGVQRQLSRLAALRDIRRLFADLGDLHTELTIAARYVLAELDQ
                     EARLLRLLASEARHGPRILTDAVVQVVDLTYREFAEWITDRSGSVVSAQQADAIATIG
                     LGALISARLLPNLLGTAHGKVDDNAIVETWVSALEHLLTTTKPNDHQAG"
     gene            1342..>1712
                     /locus_tag="VHH53_07370"
     CDS             1342..>1712
                     /locus_tag="VHH53_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2289999.1"
                     /translation="MDQSNPHLRECVAEVAVAVRGDRSVRLARGGVTALAVLFIALAM
                     AAVVVVDPRNQVVATQHTAVAGGMVAQAEYLDQPRRLGQPLTGYAGAVTGVAFSADGR
                     ILATSPIMLWDLSDRSRPRRL"
BASE COUNT          307 a          598 c          528 g          279 t
ORIGIN      
        1 gatccgctcg gatggcctga tccaccccgg gatactgcac cttgaccgcc acctgtcgcc
       61 cgtcggccag ccgtgccgcg tggacctgcc cgattgacgc cgccgcgaac gcctgcgggt
      121 cgaactctgc gaagacttcc tccggagggg cgcccagctc ggcctccacc accgccgcgg
      181 ccagttcggg cgccattggc ggcgcgtcgg cctgtagccg ggccatcgct gcctggtaga
      241 cgcgctgccg ctccgggggc accagcttcc cgagcgcgac gaaggacagc acctgcccgg
      301 ccttcatcag cacacccttc gagtggccga gcatggccgc gtagcgctca ctcgtgcgct
      361 cgtggaactc ggcgctgtcc tcgccggtca accgcttgcg cagcgcggcg acaaccgcct
      421 caccagcggt ccggccggcc agcccggcca ggggaaccac gcggcccagc cgacttccac
      481 catgcgcggt catcgccgct cctgaagttg acgcttgaca tgcctaagta aatactttgg
      541 ggccatgagc gatctgtcaa ccgtggcgac ccgggatcgc atcatcgacg cggcaatgga
      601 gctgttcagc gaacatggct accgcggtac cagcatcacc cggatcgaac aagctgccgg
      661 gctcagtcct ggcgccggcg gcatctacca ccattttccg tccaaggaag cactgctgac
      721 cgccggagtg caacggcagc tgagccgact tgccgccctg cgcgacattc gccggctgtt
      781 tgccgacctc ggagatctgc acaccgaact caccatcgcc gcccgatatg tccttgccga
      841 gctagaccaa gaagcccgac tccttcggct gcttgccagc gaggctcggc atggaccgag
      901 aatcctcacc gacgccgtcg tccaggtcgt cgacctgacc taccgcgagt tcgctgagtg
      961 gatcaccgac cgatcaggat ccgttgtatc cgcccagcag gctgatgcca tcgcgacgat
     1021 cggcctcggc gccctgatct ctgcacgact acttcccaac ctgctgggca ccgcgcacgg
     1081 caaggttgac gacaacgcca tcgtggaaac ctgggtaagc gccctcgagc acctgctgac
     1141 aacgactaaa cccaacgacc atcaggccgg ttagtaccaa aatgacaaca gagccctatc
     1201 aacgggaaca atcgaaggta attcgccgat acttggcaga cggagtcaca tcaggacctg
     1261 ccccgggggg acagggaagg cgcgatggct gccagctcaa gcgagtcacg gccgcctcga
     1321 cgtcttgagc gaggtggaca ggtggatcag tccaacccgc acttgaggga gtgcgtggcc
     1381 gaggtcgctg tggccgtacg tggagaccgc agtgtgcggc tggctcgtgg gggcgtcacc
     1441 gcactggcag tgctgtttat cgcgttggca atggccgccg tggttgtagt ggatccgcga
     1501 aaccaggtcg tcgcgactca acacaccgcc gtcgccggcg gcatggtggc tcaggccgag
     1561 tacctggacc aaccccgacg ccttggccag cccctcaccg gctacgctgg cgcggttacc
     1621 ggggtggcgt ttagcgctga cgggcgcatc ctggccacta gccccatcat gctgtgggat
     1681 ttgagcgatc gcagccgacc tcgacggctg ga
//
LOCUS       DATADN010000573         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_379962, whole genome shotgun sequence.
ACCESSION   DATADN010000573 DATADN010000000
VERSION     DATADN010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379962"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..449
                     /locus_tag="VHH53_07375"
     CDS             39..449
                     /locus_tag="VHH53_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX2290000.1"
                     /translation="MDLDEARAVVAEQHHAVLATLRRDGTPQMSPVAATVDGQGRVVV
                     STRQTAFKVRNLRRDPRVWLCVLPDGFHGRWIQVGGSVRIVELPEAMEPLVDYYRRVS
                     GEHPDWDDYRTAMERDQRVLLQIELSSAGPDREG"
     gene            549..1088
                     /locus_tag="VHH53_07380"
     CDS             549..1088
                     /locus_tag="VHH53_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HEX2290001.1"
                     /translation="MTARDLVAHLVVRESRPDVAGGILLPALARYTDRVQSSIAQRPW
                     TELVDRVRSGPPWWSPWRLPWVGDLGNTMEFFVHHEDVRRARTGWEPRPADSRRAGVL
                     WGLLRAARVLYRKSPVGVVLRTPDGSERTARRGERSVTVVGSPEELTLHAFGRDQAIV
                     DIEGDQVAVAALQGSQRGL"
     gene            1147..>1759
                     /locus_tag="VHH53_07385"
     CDS             1147..>1759
                     /locus_tag="VHH53_07385"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX2290002.1"
                     /translation="MSTAPTSTAGRPFGRVLTAMVTPFTAQGQLDLDTAQKLAHQLVE
                     KGCDGLVVNGTTGESPTTTDTEKANLVRAVVQAVGDRAAVVAGAGTYDTAHSVQLARQ
                     AAQAGAHGLLVVTPYYSRPPQAGLVAHFTAVADATELPVMLYDIPSRSLVPIATETIY
                     QLAAHPRIVAVKDAKGDFGCGAEVIANTDLAYYCGDDVLNLPWL"
BASE COUNT          274 a          575 c          643 g          267 t
ORIGIN      
        1 ccgctcgtgg cagagggatg accgcgtagc gtgcaatcat ggacctagac gaggcacgcg
       61 ccgtcgtcgc cgagcagcac cacgccgtgc tggccacgct gcgccgggac ggaacaccac
      121 agatgtcccc ggtagcagcg acggtcgacg gccagggacg ggtggtcgtg agcactcggc
      181 agaccgcgtt caaggttcgt aacctgcgtc gagacccccg tgtgtggctc tgcgtgctgc
      241 ctgatgggtt ccacggccgc tggattcagg tcggtggatc ggtgcggatc gtggagctgc
      301 ccgaggcaat ggaacctctg gtggactact accggagagt ctccggcgag catccggact
      361 gggacgacta ccgcacggcg atggagcgcg accagcgggt gctcctgcag atcgagctca
      421 gctcagcggg gcccgaccgg gagggatgac gtgacaatgg ggggtagcac tgttgccgcc
      481 cgcgagcggc acgcgttgcg caacctgatg gatgaggtgg gtccggaggc cccgacgctg
      541 tgcgctgggt gacggctcgc gatctggtgg cgcatctggt ggtccgggag agccgaccgg
      601 acgtcgccgg cggcatcctg ttgccggcgc tggctcgcta caccgaccgg gtgcagtcga
      661 gcatcgcgca acggccctgg acggagctcg tcgaccgggt ccggagcggg ccaccgtggt
      721 ggtcgccgtg gcggctgccc tgggtgggcg acctgggcaa caccatggag ttcttcgttc
      781 atcacgagga cgtgcggcgc gcccgaacgg gatgggagcc gcgaccggcc gactcccgcc
      841 gcgccggggt gctgtggggt ctgctccggg cggcccgggt gctgtaccgg aaatcgccgg
      901 tcggcgtggt gctgcggacg cccgacgggt ccgagcggac cgcccggcgc ggtgaacgct
      961 cggtcaccgt ggtcggcagc cccgaggagc tgacgttgca tgccttcggc cgcgatcagg
     1021 cgatcgtcga catcgagggc gatcaggtgg ccgtagccgc tctgcagggc agccagcgag
     1081 ggctgtagcg aacccaccca cctcggcgaa cgagggttga agcgccgacc ggtagcgtct
     1141 gcgcacatga gtactgcgcc gacctcgacg gccgggcgac ctttcggccg ggtgctcacc
     1201 gccatggtca cccctttcac ggcccaggga caattggatc tcgacaccgc tcagaagctc
     1261 gcccaccagt tggtggagaa gggttgcgac gggctggtcg tcaacggcac tacaggcgag
     1321 tcaccaacca ccactgacac cgagaaggcg aatctggtgc gggcggtggt gcaggcggtg
     1381 ggagaccgag cggccgtggt ggccggcgcg ggaacctacg acaccgcgca cagcgtgcag
     1441 ctggcccggc aggctgcgca ggccggcgcg cacggcctgc tagtggtgac cccgtactac
     1501 tcccgcccgc cacaggccgg gctggtcgcg cacttcactg cggtcgccga tgccaccgag
     1561 ctgccggtga tgctctatga catcccctcg cgctcccttg ttccgatcgc cacggagacc
     1621 atctatcagc ttgctgcgca cccgcggatc gtcgcggtca aggacgccaa gggagatttc
     1681 ggctgcggcg ccgaggtgat cgccaacacc gacctggcct actactgcgg ggacgacgtg
     1741 ctcaacttac cgtggctgt
//
LOCUS       DATADN010000574         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_526628, whole genome shotgun sequence.
ACCESSION   DATADN010000574 DATADN010000000
VERSION     DATADN010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526628"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VHH53_07390"
     CDS             complement(<1..381)
                     /locus_tag="VHH53_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX2290003.1"
                     /translation="MQRWRGLEAIPNGWGRSVVTIGVFDGVHRGHAQLIGQAVRRARE
                     LSVPCVLMTFDPHPSEVIRPGSHPAQLTTLRRRAELVEQLGADVFFVLPFTVELSRMP
                     ADEFVHEVLVERLHVAEAVVGDNFT"
     gene            complement(415..1320)
                     /gene="truB"
                     /locus_tag="VHH53_07395"
     CDS             complement(415..1320)
                     /gene="truB"
                     /locus_tag="VHH53_07395"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX2290004.1"
                     /translation="MSFTDVPSGLVVVDKPAGMTSHDVVARLRRLLNIRRIGHAGTLD
                     PMATGVLVCGVQRATKLLGHLPLEPKVYLATVRLGVSTSTDDADGECTGDIDASTVSE
                     PAILAGIADLSGAIEQVPSSLSAVKVGGRRAYTLARAGEQVSLAARPVNVSRFELLRL
                     RRGAAVTDLDVLVECSGGTYVRALARDLGAALGVGGHLIALRRIRVGPFDVSSARTLD
                     DLAATPGLSVQLDEVVATAFPHRAVDATQARLLAHGGGLPAGGLAGTHAAIDSDGHAV
                     ALLAERAGQARPVLVLRPAGDRCGR"
     gene            complement(1317..2375)
                     /locus_tag="VHH53_07400"
     CDS             complement(1317..2375)
                     /locus_tag="VHH53_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2290005.1"
                     /translation="MIDVIIAGGGPTGMMLARELRLHGVHALVLEKEAAPTGHARALG
                     LHVRSIEVMDQRGLLERFLAHGKKYAGSSFFAGIEKPWPDRLDTAHPYVLGIPQTTTE
                     RLLTEHATELGVKIRRGYELVGLSQDDHGVTAELADGTQLRSRYLVGCDGGRSTVRKL
                     LGVGFPGEPTRVETLLGEMEVTEDPATVAAIAAQSLVGAALGAGNESSARSLAWQITR
                     YGLAFGTALGLVFAAAQPVLPQVFTSDSAVLAEVPHAWWFFVALQPVAGVVFALDGVL
                     LGAGDAAFLRTCTLISALVGFLPVVWASFVCGWGLIGIWSGLAAFMVLRLAFVLARTY
                     SPGWVVTGAVRGPGRIAP"
BASE COUNT          437 a          940 c          838 g          363 t
ORIGIN      
        1 ggtgaagttg tctcccacga ccgcctcggc cacgtgcagg cgctcgacaa gtacctcgtg
       61 gacgaactca tcggccggca tccgggacag ttcgacggtg aacggcaaca cgaagaacac
      121 gtcggccccg agctgttcga ccagctcggc ccggcggcga agggttgtga gctgcgctgg
      181 gtggctgccg ggacggatga cctccgaggg gtgcgggtca aaggtcatca acacgcaggg
      241 aacgctcagt tctcgggcgc gccgcacggc ctgcccgatc agctgggcgt gaccgcggtg
      301 caccccgtcg aacaccccga tggtgaccac gctgcggccc caaccgttcg ggatggcctc
      361 tagtccccgc cagcgctgca cagacgatga ggctaatcgg tctcaggtgt ccaatcaccg
      421 cccgcaccta tcccctgctg gacgcagcac caggacgggc cgggcttgcc cagcgcgctc
      481 ggcgagcaac gcgacggcgt ggccatcgga gtcgatcgcg gcgtgcgttc cggccaaccc
      541 gcccgccggc agtcccccgc catgggcgag taagcgggcc tgggtggcgt ccacggcgcg
      601 gtgcgggaac gcggtcgcca ccacctcgtc cagctgcacc gatagacccg gcgtggccgc
      661 gagatcatcg agggtacggg cgctgctcac atcgaaggga cccacccgga tgcggcgcag
      721 agcaatgaga tggcccccga ctcccagcgc ggcgccgagg tccctggcca acgctcgaac
      781 gtaggtgccc ccgctgcatt ccaccagcac gtcgaggtcg gtcaccgcgg cgccgcggcg
      841 cagcctgaga agctcgaatc gcgacacgtt caccggtcga gcggccagtg acacctgctc
      901 accggcccgg gccagtgtgt aggctcgcct gccgccgacc ttgaccgcgc tcaacgagct
      961 gggtacctgt tcgatggcgc cgctgaggtc cgcgataccc gccagaatcg ccggctcgct
     1021 caccgtggat gcgtcgatat ccccggtgca ttcgccgtcg gcatcgtcgg tgctggtgga
     1081 cacaccaagc cgcaccgtgg ccaagtagac cttgggctcc aacggaagat gcccgagcaa
     1141 cttggtggcc cgttgcacgc cgcaaacgag cacgccggtg gccatcggat ccaacgtgcc
     1201 agcgtgcccg atccggcgga tgttcagcag tcgccgtagc cgagctacca catcatgtga
     1261 tgtcatcccg gccggcttgt ccaccaccac caggccgctg ggtacgtccg tgaagctcat
     1321 ggcgcaatcc tgccgggacc gcgcacggcg ccggtcacca cccagcctgg gctgtacgtc
     1381 cgggccagca cgaacgccaa gcgcagcacc atgaacgcgg ccagcccgga ccagatgccg
     1441 atcaaccccc aaccacacac gaacgatgcc cataccactg gcaggaatcc gaccagcgcg
     1501 ctgatgaggg tgcaggtgcg caggaacgcc gcgtctcctg caccgagcag taccccatcc
     1561 agcgcgaaca ccacgccggc cacgggctgc agggcgacga agaaccacca ggcgtgcggt
     1621 acctcggcca gcacggcgga gtcggaggtg aacacctgtg gcagcaccgg ctgggcggcg
     1681 gcgaacacca gcccgagggc ggtcccgaac gccaacccgt aacgggtaat ctgccaggcc
     1741 agcgagcggg cgctggactc gttgccggcg cccagcgcag cgccgaccag tgactgcgcg
     1801 gcgatggcgg caaccgtcgc cggatcctcg gtcacctcca tctcgcccag cagcgtctcg
     1861 accctggtgg gctcgccggg gaagccgacg ccgagcagct tgcgtaccgt gctgcggccg
     1921 ccgtcgcagc cgacgaggta gcgcgagcgc agctgcgtgc cgtcggccag ctcggcggtc
     1981 accccgtggt cgtcctggct tagcccgacc agttcgtagc cgcgtctgat cttgacgccg
     2041 agttcggtgg cgtgctcggt cagcaggcgc tcggtggtgg tctgcgggat gccgaggacg
     2101 taggggtgcg cggtgtccag ccggtcgggc cacggcttct cgatgcctgc gaagaatgag
     2161 gagccagcat acttcttgcc gtgtgcgagg aaccgctcca gcagaccgcg ctggtccatc
     2221 acctcgatgc tgcgcacgtg caggccgagc gcacgggcat gcccggtcgg cgccgcttcc
     2281 ttctccagca cgagcgcgtg cacgccgtgc agccgcaact cgcgggccag catcatgccc
     2341 gtcggtccgc cgccggcaat gatcacgtca atcatggaac cccattccaa tgagtgaatt
     2401 tcggccagct gctccgctca gtccgtcggc gcgcacaccc ccacctccgc gccgagtgcc
     2461 cgcaaccgca ccgtgagtcc caccggcggt tcgacgtccc cgcgtccccc atacgtcgac
     2521 accaacacac gcacgtcgcg actcccattt ccccgagtac tggcttcggc cggcgaat
//
LOCUS       DATADN010000575         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1133156, whole genome shotgun sequence.
ACCESSION   DATADN010000575 DATADN010000000
VERSION     DATADN010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133156"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VHH53_07405"
     CDS             complement(<1..225)
                     /locus_tag="VHH53_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX2290006.1"
                     /translation="MVSVVRSRSADEFVHPALFYRGTAEYLKGTVPFIRDGLAVGEPV
                     AIAVPGPNLRLILTELGADAGRVRLLDMIRA"
     gene            531..1022
                     /locus_tag="VHH53_07410"
     CDS             531..1022
                     /locus_tag="VHH53_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX2290007.1"
                     /translation="MTAIENLVGRNAQRAGPRPGTMAARPGLRIAVVACMDARIDPVA
                     LLGLQPGEAHVIRNAGGVVTDDVIRSLAVSQRKLGTTEVMLLHHTKCGMATFTDEDFW
                     SELQEATGLRPSWAVETFRDTESDVRQSVERVRRSPFLANTTAVRGFVIDVETGDLTE
                     VPA"
     gene            985..1905
                     /locus_tag="VHH53_07415"
     CDS             985..1905
                     /locus_tag="VHH53_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX2290008.1"
                     /translation="MSRPVTLPRYRHDTREHNSVNPAQLIAWFDAHSRELPWRKPGTT
                     PWGVLVSEVMLQQTPVHRVAGVWSEWMVRWPAPSSLATEIPGTAVRAWGKLGYPRRAL
                     RLHAAAVVIATRYDDIVPADVDVLLTLPGIGAYTARAIAAFGYGQRVPVVDTNVRRVV
                     ARAVRGMAGPAAPSTSRDLSNVDGVLPETQAARFCAALMELGAIVCTARHPRCASCPV
                     AGDCAWIRAGKPDGTGSGRPVQRFAGTDRQVRGLLLDVLRSTAVPVPRTRLDAVWVDA
                     TQRDRCLGSLLVDGLVEQTVDGCFALPGER"
     gene            1934..2251
                     /locus_tag="VHH53_07420"
     CDS             1934..2251
                     /locus_tag="VHH53_07420"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX2290009.1"
                     /translation="MAVVKINAIEVPEGAGPELERRFAARHGSVESAPGFLGFELLRP
                     VAGDERYFVYTKWETEEHFQAWRNGPAMEAHSGARAKPVATGSSLLEFEVVQSAEPSA
                     GTP"
     gene            complement(2297..>3705)
                     /locus_tag="VHH53_07425"
     CDS             complement(2297..>3705)
                     /locus_tag="VHH53_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX2290010.1"
                     /translation="ALVAAATLADRYINDRFLPDKAIDLVDEAGARMRIRRMTAPPDL
                     REFDEKIAAVRRDKESAIDAQDFEKAAKLRDSEKQLLVKKSEREKQWKAGDLDVVAEV
                     DEEQIAEVLANWTGIPVFKLTEEETTRLLRMEDELHKRIIGQHEAVKAVSKAMRRTRA
                     GLKDPKRPSGSFIFAGPSGVGKTELSKALAEFLFGDDDALIQIDMGEFHDRYTASRLF
                     GAPPGYVGYEEGGQLTEKVRRKPFSVVLFDEIEKAHQEVYNTLLQVLEDGRLTDGQGR
                     TVDFKNTVIIFTTNLGTSDISKAVGLGFSVGNDESSNYERMKSKVNDEVKKHFRPEFL
                     NRIDDTIVFHQLTEEEIIRMVDLMISRVEVQLRNKDMELELTTMAKRLLAKRGFDPVL
                     GARPLRRTIQREIEDQLSEKILFGEIEPGQIVIVDADNWDGEGPTEDARLVFRGEPKP
                     LRVPDALPVDLGSAPSAD"
BASE COUNT          623 a         1220 c         1192 g          670 t
ORIGIN      
        1 ggcgcgaatc atgtcaagca ggcggacccg cccggcatcg gcgcccaact cggtgaggat
       61 gagtcgcagg ttcggacccg gcacggcgat ggcgacgggc tcgccgacgg ccaacccgtc
      121 gcgaatgaag ggcaccgtgc ccttgaggta ctccgcggtg ccgcggtaga acagcgctgg
      181 gtgtacgaac tcatcagcgg agcgagaacg gaccacactc accacctcgt ctcgatgcga
      241 ccagcaacgt atgcccgccg acggatcggc aggcatcatc accgcgcctc gccccactgc
      301 ccagagtagg tagtaaaccg ggcagttaca acccgattcg gtttgctcac agtaagttgc
      361 cgtcaagtac ccgaattgcc gatggcacag cgcggtgccc acgaagtcgg cacatctgcc
      421 ggggagagaa cccgaccggt cgctgcggcc ccgaacctac gaggtgcccc cgctgccagc
      481 acaatattgc gtccaggctt gcatcccagc tggcaacgaa ggagatgtcc atgacggcga
      541 tcgagaactt ggtgggccgc aacgcacagc gcgcgggtcc ccgacctggc acgatggcgg
      601 ctcggcctgg cttgcgcatc gctgtggtgg cctgcatgga cgcccggatc gacccagtcg
      661 cgctgctcgg cctgcagcca ggtgaggcac acgtgattcg caacgcaggc ggcgtcgtga
      721 ccgatgacgt cattcgctca ctggccgtca gtcagcgaaa gctcggcacc accgaggtga
      781 tgttgctgca ccacaccaag tgtggaatgg cgacgttcac tgacgaggac ttctggtccg
      841 aacttcagga ggccaccggc ctgcggccca gttgggcggt cgagactttt cgcgacaccg
      901 aatccgacgt gcggcagtcg gtggaacggg tccggcgtag cccgttcctg gcgaatacca
      961 ccgcggtgcg cggctttgtc atcgatgtcg agaccggtga ccttaccgag gtaccggcat
     1021 gacacccgtg aacacaactc cgtgaacccg gcacagctga ttgcgtggtt tgacgcgcac
     1081 tcgcgtgaac tcccgtggcg caagcccggc accacgccgt ggggtgtgtt ggtcagcgag
     1141 gtcatgctgc agcaaactcc ggtgcatcgc gtcgccggcg tgtggagcga atggatggtg
     1201 cgctggcccg ccccctccag cttggccacc gagataccgg ggaccgcggt ccgcgcttgg
     1261 ggaaagctgg gctacccgcg gcgggcgctg cggctacatg cagctgccgt agtgatcgcc
     1321 acgcggtacg acgacatcgt gccggcggac gtggatgtcc tgctcaccct gcccggaatc
     1381 ggtgcctaca ccgcccgagc gatagcggcc tttgggtacg ggcagcgggt gccggtggtg
     1441 gacaccaacg tgcggcgggt tgttgctcgt gcggttcgag gcatggccgg ccccgccgcg
     1501 ccgtccacgt cacgagatct gtctaacgtg gatggtgtgt tgcccgagac gcaggccgcc
     1561 cgcttctgcg ccgcgctgat ggaactcggc gcgatcgtgt gcaccgctcg ccatccgcgc
     1621 tgcgccagct gtccggtggc cggcgactgc gcctggataa gggccggcaa gcctgatggg
     1681 accggctccg gtcggccagt gcagcgcttt gcaggcaccg atcgccaagt gcgcggtttg
     1741 ctgctcgacg tgctgcgatc gacggcggtc cccgtgcctc ggacccgtct ggacgcggtg
     1801 tgggtcgacg ccacacagcg agatcggtgc ctgggctcgt tgctcgtcga cggcctggtg
     1861 gaacagaccg tcgacggctg cttcgccttg cccggcgagc gctgagcggt gcgagcgccc
     1921 gtaggctagc tacatggctg tcgtgaagat caatgctatc gaggtgccag aaggcgcggg
     1981 tcccgagctg gagcgccgat tcgccgcccg ccatggttca gtggaatccg cccccggctt
     2041 cctgggcttc gagctgctac gcccggtggc cggggatgag cggtacttcg tctacaccaa
     2101 gtgggagact gaagaacact tccaagcctg gcgcaacggc cccgcgatgg aagcacactc
     2161 cggcgcgcgg gccaaaccgg tagccaccgg ctcctccctg ctcgagttcg aggtcgtcca
     2221 gtcagccgag ccttctgccg gtactccttg accgccattc ggagccgaat ggcgggcaca
     2281 ggacggacga atcggatcag tcggcgctgg gggcgcttcc caggtcaacc ggtagcgcgt
     2341 cgggtacccg caacggcttt ggctcgccgc ggaacaccag cctggcgtcc tcggtggggc
     2401 cctcgccgtc ccagttgtca gcatcgacaa tgacgatctg acccggctcg atctcgccga
     2461 acaggatctt ctccgacagc tgatcctcga tttcgcgctg gatggtccgg cgtagcggtc
     2521 gggcccccag cacaggatcg aatccgcgct tggccaggag cctcttggcc attgtggtga
     2581 gctccagctc catgtccttg ttgcgcagct ggacctcgac ccgggagatc atcaggtcca
     2641 ccattctgat gatctcttcc tcggtgagct gatggaaaac gatggtgtcg tcgatccggt
     2701 tgaggaactc cggacggaaa tgcttcttga cctcgtcgtt gaccttgctc ttcatccgct
     2761 cgtagttcga gctctcatca tttccaaccg agaagcccag accgacagcc ttggagatgt
     2821 ccgaagtgcc gaggttggtg gtgaagatga tcacggtgtt cttgaaatcc accgtacggc
     2881 cctgaccgtc ggtcagccga ccatcctcca gcacctgcag cagcgtgttg tatacctcct
     2941 ggtgcgcctt ctcaatctcg tcgaagagca ccaccgagaa cggcttgcgg cgcaccttct
     3001 cggtgagctg accgccttcc tcgtaaccca cgtatccggg cggggcgccg aaaagccggg
     3061 atgcggtgta gcggtcgtgg aactcaccca tgtcgatctg gatgagcgcg tcgtcatcac
     3121 cgaacaggaa ttccgccagc gccttggaga gctccgtctt acccacaccg gaggggccag
     3181 cgaagatgaa agacccggac ggccgcttgg gatccttcag acctgcacgg gtacggcgca
     3241 tcgccttgga aacggccttg accgcctcat gctggccgat gatccgcttg tgcagctcgt
     3301 cctccatgcg caacagccga gtggtctcct cctcggtgag cttgaacacg gggatgccgg
     3361 tccagttcgc cagcacctcg gcgatctgct cctcatcgac ctcggcgacg acgtcgaggt
     3421 caccagcctt ccactgcttc tcccgctcgg acttcttcac cagcagctgc ttttcggaat
     3481 ctcgcagctt cgcggctttc tcgaagtcct gggcatcgat ggccgactct ttgtcccggc
     3541 gcaccgcagc aatcttctcg tcaaattcgc ggaggtccgg cggagcggtc atccggcgga
     3601 tccgcatccg ggcgcccgcc tcgtcgacca ggtcgatcgc cttgtccggc aggaagcgat
     3661 cattgatgta ccggtcggcc agggtcgcgg cggcgaccag tgcgc
//
LOCUS       DATADN010000576         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1263965, whole genome shotgun sequence.
ACCESSION   DATADN010000576 DATADN010000000
VERSION     DATADN010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263965"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1658)
                     /gene="dop"
                     /locus_tag="VHH53_07430"
     CDS             complement(147..1658)
                     /gene="dop"
                     /locus_tag="VHH53_07430"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEX2290011.1"
                     /translation="MRRIMGTEVEYGISVPGDCSANPVLTSTQVVLAYAAAAEVPRAK
                     RARWDYEVESPLRDARGFDLGLPGIAQPDPELDDLGAANVILTNGARLYVDHAHPEFS
                     APEVTNPRDAVIWDKAGERIMEEAALRARTVPDQPPFQLYKNNVDGKGASYGTHENYL
                     MARSTPFTLVIAGLTPFFVSRQVVCGSGRVGIGPAGEEPGFQLSQRADYIEVEVGLET
                     TLKRGIINTRDEPHADADKYRRLHVIIGDANLAEYSTYLKIGTTALVLDLIEAGERFD
                     DLRLVDAVRAVHTISRDPSLQATVEVSGGRHLTGLDLQRAYHERVAHFFAREGVPDNT
                     ADIAEHILSTWGEVLDALARDPMECADRLDWPAKLQLLEGYRARDGLAWASPRLHLVD
                     LQYSDVRMHKGLYNRLVARGSMKRLVTEEEVRAAITAPPEDTRAYFRGRCLERYATEV
                     AAASWDSVIFDLGRESLVRIPTLEPLRGTRAHVGALLDASATAAELVEKLTRG"
     gene            1845..>2252
                     /locus_tag="VHH53_07435"
     CDS             1845..>2252
                     /locus_tag="VHH53_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX2290012.1"
                     /translation="MTTATSTAIPGYVAGIWDIDSTHSDVSFSIRHMMVSKVRGRFNN
                     VSGRLVTGADLLDSSVTADIDVNSFDTGNAQRDAHIQSADFLHATQYPTMTYRSTGIR
                     LDGDDLVVDGELTLHGITRQVPLALEVTGFGPDP"
BASE COUNT          364 a          777 c          724 g          387 t
ORIGIN      
        1 gtgcgccgcc gcccgtctcg tcgtcgccgt cgccgccgcc ttggcgctgc accttctcct
       61 gggacatctc gcctcccgga ccgaaagttg tctccagcaa acagtaccca gcaaacccga
      121 caatcgctgc caggccagtc gggttatcag cctctggtga gtttctccac cagctccgca
      181 gcggtggccg aagcgtccag cagcgccccg acatgcgcac gggtgccgcg cagcggttcc
      241 agggtgggga tccggaccag tgactcgcgg cccagatcga agatcaccga atcccaggac
      301 gcggcggcca cctctgtggc ataacgctcc aggcaccggc cgcggaaata ggcacgggtg
      361 tcctccgggg gcgccgtgat ggctgcgcgt acttcctcct cggtgaccag gcgtttcatc
      421 gagccacggg ccaccaggcg gttgtacagc cccttgtgca tccgcacgtc ggagtactgc
      481 agatccacca ggtgcagccg aggtgatgcc catgccagac cgtcgcgggc ccggtagccc
      541 tcaagcagct gcagcttggc cggccagtcc agccgatcgg cgcactccat cgggtcccgc
      601 gccagggcgt cgagcacctc gccccaggta ctgaggatgt gctctgcgat gtcggcggtg
      661 ttgtccggca cgccctcccg ggcgaagaag tgcgcaaccc gttcgtggta agcccgttgc
      721 aggtccaggc cggtgaggtg gcgaccacca gacacttcga ccgtcgcctg cagggacggg
      781 tcgcggctga tggtgtgcac tgcccgcacc gcgtcgacca accgcaggtc gtcgaagcgt
      841 tccccggcct cgatcaggtc cagcaccaac gccgtggtgc cgatcttcag ataggtggag
      901 tactcggcaa ggttggcgtc gccgatgatg acgtgcagcc ggcggtactt gtcggcgtcg
      961 gcgtgcggct cgtcccgggt gttgatgatg ccccgcttga gtgtggtctc cagcccgacc
     1021 tcgacctcga tgtaatccgc ccgctgggac agctggaatc ccggctcctc ccccgccggt
     1081 ccgataccta cccggcccga tccgcagacc acctggcggg acacgaagaa cggggtcaac
     1141 ccggcaatga ccagggtgaa cggcgtgctg cgcgccatca ggtaattctc atgggtgccg
     1201 taggaggcac ctttgccgtc aacgttgttc ttgtagagct ggaagggcgg ctggtcgggg
     1261 accgtacgag cgcgcagcgc ggcttcctcc atgatccgct cgccggcttt gtcccagatc
     1321 accgcatcgc gcgggttggt gacctcaggg gcggagaact ccgggtgtgc gtggtcgaca
     1381 tacagccggg ctccgttggt caagatgacg ttcgctgcgc cgagatcgtc cagctctgga
     1441 tcgggctggg cgattccggg caggcccagg tcgaagccgc gggcgtcccg cagcggggac
     1501 tccacctcgt agtcccatcg ggcgcgcttg gctcgcggca cttcagccgc cgcggcgtag
     1561 gccagcacga cttgagtcga ggtcaggacg gggttggcgg agcagtcccc gggcaccgag
     1621 atgccgtact cgacctcagt tcccatgatc cggcgcataa cccgagggta cgtgtgtgcg
     1681 aggcaggtgg cccgccacgc ctgaatggcg gacccgtatc cgccggctct cgcaacgtcg
     1741 cagcgagcta ccttgcggtg cggaataatt taaccttcaa cttgttgtcc cgggtatggc
     1801 cgcgcagcac cgcgcggtac accgctactg atggggagtt caccatgacc accgctacgt
     1861 caaccgcgat ccccgggtac gtcgccggta tctgggacat cgactccacg cactccgacg
     1921 tctcgttctc catccggcac atgatggtga gcaaggtgcg gggccgcttc aacaacgtct
     1981 ccggccgcct ggtcaccggc gccgacctgc tggactcgtc ggtgaccgcg gacatcgacg
     2041 tgaacagttt cgacaccggc aatgcccagc gcgacgcgca catccaatcg gctgacttcc
     2101 tgcacgcgac gcagtacccc accatgacct accggtccac cgggatacga cttgacggcg
     2161 atgatctcgt ggtcgacggc gagctgacct tgcacggcat cactcgccag gtccccctgg
     2221 ccctcgaggt gaccggcttc ggcccggatc cc
//
LOCUS       DATADN010000577         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_54820, whole genome shotgun sequence.
ACCESSION   DATADN010000577 DATADN010000000
VERSION     DATADN010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54820"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VHH53_07440"
     CDS             <1..241
                     /locus_tag="VHH53_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX2290013.1"
                     /translation="GKPADISAADLIDDESLRAEVQGAVDDANKTVSRAETIRKFRIL
                     PIDFTEAGGQLTPSLKIKRAVIAKEFAAEIDSLYS"
     gene            complement(272..1402)
                     /locus_tag="VHH53_07445"
     CDS             complement(272..1402)
                     /locus_tag="VHH53_07445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2290014.1"
                     /translation="MPRILLVSNDFPPRAGGIQTYLHKLAMALPASEIAVYAPDWPGA
                     AGFDAQLPFPVIRHPGSLMLPTPAVLRRAVGLLRELRYETAWFGAAAPLALLGPALRR
                     AGVRRVVASSHGHEVGWSMLPPGRMALRRIGMDADVVTTVSRYTRRRVASAFGPLAAL
                     ELLQPGVDCAAFQPEPAARAEIRRRHRLGEAPVVLCVSRLVARKGQDTLVKALPAIRR
                     QVPGATLLLVGDGPQRAALHRLAAECGVADAVVCTGPVPWSELSAHYAAGDVFAMPCR
                     TRGRGLDVEGLGLVYLEAAAAALPVVAGDAGGARETVRPGDTGELVNGRDVEAVAKTI
                     GTLLADPERAAAMGRRGRGWMLQDWDWGCRRQKLVELLLASS"
     gene            complement(1431..2420)
                     /locus_tag="VHH53_07450"
     CDS             complement(1431..2420)
                     /locus_tag="VHH53_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX2290015.1"
                     /translation="MTVLMALIPAPAAADPAPPDDAPAAVQRLAELNHQAEVLTERWH
                     YARDQLNARRADLERAKADVTAAAASGERARAVQGQYRGQVDRLAHASFQGARLNGLS
                     AVLISDSPEEFLDQMSALDMLAADNKQALDRLTGAVVAAQQAEQTANQAAARAAQAER
                     DAARLEGDLTRSRDEMDRQIQVVQQRLGALDSEERAAYTSGGSTEFPVNLVGSGAAIQ
                     ATRAALSRQGSAYVWGSEGPITFDCSGLVKWSFEQAGMSGLPHSSQAQARMGSRVSRS
                     QLRPGDLIALYSPISHIGIYVGDGFYVNAPQPGDVVKVVRVPWGQVTAMSRIA"
BASE COUNT          464 a          964 c          873 g          428 t
ORIGIN      
        1 cggcaagccg gctgacatct ccgccgccga tctgatcgac gatgagtccc tgcgcgccga
       61 ggtgcaaggt gcggtggacg acgccaacaa gaccgtctcc agagccgaga cgatccgcaa
      121 gttccggatc ctgccgatcg actttaccga ggctggtggt cagctcactc caagtctcaa
      181 gatcaagcgg gcggtgatag ccaaggagtt cgccgccgag attgactcgc tttactcgtg
      241 agtgggaaca gtgtcataac gctgttccca ctcacgagct cgccagcagc agctctacaa
      301 gcttctgccg gcggcatccc cagtcccagt cctgcagcat ccagccccgc ccgcgccgac
      361 ccatcgcagc ggcccgctcg ggatcggcaa gcagtgtccc gatggtcttg gccaccgctt
      421 caacgtcacg gccgttcacc agctcaccgg tgtcgccagg gcggacagtc tcccgtgccc
      481 cccctgcgtc cccagcgacc acgggcaagg cggccgccgc ggcttcgaga tacaccagcc
      541 cgagcccttc gacgtccagc ccccggccac gggtccggca gggcatcgcg aagacgtcac
      601 ccgccgcgta gtgcgcggac agctccgacc acggcactgg tcccgtgcag accacggcgt
      661 cggcgacccc acattccgca gccagccggt gcaacgctgc gcgctgaggc ccgtccccca
      721 ccagcagcag cgttgcccct ggcacctggc ggcggatcgc gggcaatgcc ttcaccagcg
      781 tgtcttgtcc cttgcgggcc accaaccggg acacgcacag cacaaccgga gcctccccta
      841 gtcgatgacg ccgccggatc tctgcccgtg ccgcgggctc gggttgaaac gctgcacagt
      901 ccactccagg ctggagtagt tcaagtgcgg ccagtggacc gaacgcagac gccacgcggc
      961 ggcgggtgta ccggctcacc gtggtgacta cgtcggcatc cattccgatc cggcgcagcg
     1021 ccatccggcc aggaggcagc atggaccaac ccacctcgtg cccatggctg ctggccacca
     1081 cgcgtcgtac gcctgcgcga cgcaatgccg gccccagcag tgccagaggg gcggcggcgc
     1141 cgaaccacgc ggtctcatag cgcagctccc gtagcagccc gacggctcgt cgcagtaccg
     1201 ccggggtggg cagcatgagg gatccaggat gccgaatgac cgggaacggc aactgcgcat
     1261 cgaagcctgc cgcccccggc cagtccggtg cgtagacagc gatctcgctg gcgggtaacg
     1321 ccatggccag cttgtgcagg taggtctgga tgccgccggc ccggggcggg aagtcattgc
     1381 tcaccagcaa gatgcgtggc atcgccggag ggtagccgga tctggtgtgc tcaggcgatc
     1441 cggctcatcg cggtgacctg accccacgga actcgaacca ctttgaccac gtcaccgggc
     1501 tgcggtgcgt tcacatagaa cccgtcaccg acatagatgc cgatgtggct gatgggtgag
     1561 tacagcgcga tgaggtcgcc cggccgcagc tgcgagcggc tgaccctgct ccccattcgc
     1621 gcctgtgcct gggacgagtg cggtaggccc gacatgccgg cctgctcgaa ggaccatttc
     1681 accagtcctg agcagtcgaa cgtgatcgga ccttcgctgc cccacacgta cgccgagcct
     1741 tgcctactca acgcggcccg cgtcgcctga atcgcggcac cgctgccgac gaggttgacg
     1801 gggaactcgg tactaccacc ggacgtgtag gcggcccgct cctcactgtc gagcgccccg
     1861 aggcgctgct ggaccacctg gatctgacga tccatctcgt cgcgggaacg ggtcaggtcg
     1921 ccttcgagcc gcgccgcatc gcgctcagcc tgggcggccc gcgctgctgc ctggttagct
     1981 gtctgctcag cctgctgggc cgcaacaacg gcaccagtga ggcggtccag ggcctgtttg
     2041 ttgtccgccg cgagcatgtc caacgccgac atctgatcca ggaactcctc gggggagtca
     2101 ctgatcaata cggcggacag cccattgagg cgcgcaccct ggaaggaggc gtgggccagc
     2161 cggtcgacct gaccgcggta ctgaccctgg accgccctgg cacgttcccc tgaggcggcc
     2221 gcggcggtga cgtcggcttt ggcccgctcc agatcggccc ggcgggcatt gagctggtca
     2281 cgggcgtagt gccaccgctc ggtgagcacc tcagcctggt ggttcagctc cgcaagccgt
     2341 tgcacagccg ccggggcgtc gtcgggtggt gcagggtcgg ccgcagccgg agcggggatc
     2401 aaggccatca ggacagtcat ggcaccggta ataaccgctg ccggcacgct gcgcttcaga
     2461 cggtgcgatg acacgtcccg tctgtctcct cttccctcgg accgcctacc ggattaaccg
     2521 aacggattca ggcgggtgag cggacgtcga cccgtacatc tgggcgcgaa cgctcatccg
     2581 gattcatccc gaaagagttg gatccccggc tcggctgtcg tatggcagca ccgacgaagc
     2641 cgattcggca gcgacctgcg tgcctccagg cttggaagca gctaccgcta agtcgcggca
     2701 aggttacgaa aggtcacttg cactgtcca
//
LOCUS       DATADN010000578         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_983077, whole genome shotgun sequence.
ACCESSION   DATADN010000578 DATADN010000000
VERSION     DATADN010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983077"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VHH53_07455"
     CDS             complement(<1..291)
                     /locus_tag="VHH53_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290016.1"
                     /translation="MSTRTGGTVDIGVLWTRRLGMPDGPFGSGSNPLEDLVSRLVGGL
                     EQSLGWLTKPATSTQRVDRYGRDLTAEARAGRLDPVIGREDEIEQILEVLSRR"
     gene            396..1202
                     /locus_tag="VHH53_07460"
     CDS             396..1202
                     /locus_tag="VHH53_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2290017.1"
                     /translation="MPDNGGASRRAAMYGDDDLSSFSLFSGNYINFGYWQDFMPGLIS
                     VEERTESQADLYRNVLRRLSIGPNDVVLEIGCGIAVGAALALREFHPGALYGLDLSQD
                     QINRASRVNSELLTQQRGRFMLQQGSALQLPYADEHFNKCYSVEAAQHFESLATFASE
                     AYRVLRPGGRLAVATFFMTHASAADELRQLIETIDSGIDVVVAIGSFRDDLLNAGFAE
                     VDVDSIGEHVWCGFDAWMEQTEFKDSWGRNWLKAYQRGLMDYYLVTADKK"
     gene            1234..1539
                     /locus_tag="VHH53_07465"
     CDS             1234..1539
                     /locus_tag="VHH53_07465"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2290018.1"
                     /translation="MAAWRAVTDSEPDFAQRAQTLRADGSPRISGIEASFVRGQLWFG
                     SMPDARKGRDLRRDPRFALNFRADIDEVVITRVGEPVDHLVIELWPPDRPLHRIQRH"
     gene            complement(1547..2962)
                     /locus_tag="VHH53_07470"
     CDS             complement(1547..2962)
                     /locus_tag="VHH53_07470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX2290019.1"
                     /translation="MKAPRKPSRPSRPGPPRRRSGPRRPPVEDLPRRAALDALAAVRE
                     RDAYVNLVLPGLLRQRGISGRDAALATELCYGTCRAQGLLDAVLESCTDRPLSEVDGE
                     LLDALRLGSYQLLRTRVPSHAAVGTTVDLVRADRGSASAGFVNAVLRRVSQHDEQGWV
                     QAVAPAAGDDLVGYLAMAHPHPRWIAQAFSDALGGDSDELTEALAADDARPLVHLAAR
                     PGRITATDLAAVTGGEAAPYSPYGVHLADGGDPGELAAVRDHLAHVQDEGSQLVALAL
                     AGVQLEGADVRWLDLCAGPGGKAALLGALAAARGASLDAVEKAEHRARLVGQACVGLP
                     VTVHVADGRNSQLPERSYDRVLVDVPCTGLGALRRRPEARWRRRADDLAGLVRLQREL
                     LLAALRLVRPGGVVAYVTCSPHRDETVGVVADVVRRTGVEQLDARQQFSDVPRLGDGP
                     HVQLWPHRHGTDALFCALLRR"
     gene            complement(3003..>3111)
                     /locus_tag="VHH53_07475"
     CDS             complement(3003..>3111)
                     /locus_tag="VHH53_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX2290020.1"
                     /translation="SSAVRLGEVTAPGKRAMPAPDWARGARLAPGERFQ"
BASE COUNT          533 a         1083 c         1016 g          479 t
ORIGIN      
        1 acgccgcgac agtacctcca ggatttgctc gatctcatcc tcccgcccga tgacggggtc
       61 gaggcggccg gcgcgagcct ccgcggtgag gtcgcggccg tagcggtcca cgcgctgcgt
      121 cgatgtggct ggcttcgtta accaaccgag ggactgctca aggccgccga ccagccgact
      181 caccaggtcc tcgagtgggt tggaccccga gccgaaaggc ccatcgggca taccgagcct
      241 cctcgtccac agcaccccga tgtcgacggt accgcccgtt cgggtcgaca cgcagctcgt
      301 cagcgccccg gtttccttcc agacagccga tctaggatga ccggcggctg gtaaaccggt
      361 gcgacacagc atcgaggcgg agggcgagag gaatcatgcc agataacggt ggtgcttccc
      421 ggcgggccgc gatgtacggc gacgacgatc tcagctcgtt ttcactcttc tcaggtaact
      481 atatcaactt cgggtattgg caggatttca tgccggggct catcagcgtc gaggagcgca
      541 ctgagagcca ggccgacctt taccgcaacg tccttcgccg cttgagcatc ggcccgaacg
      601 atgtggtgtt ggaaatcggg tgtggcatcg cagtaggtgc agcacttgct ctccgagaat
      661 tccacccagg cgcactgtac ggcctcgatc tctcgcaaga ccagatcaat cgcgccagca
      721 gggtcaactc cgaattactc acccaacagc gtggccggtt catgctccag caaggctccg
      781 cgctccaact accttatgcg gatgagcact tcaacaagtg ctactcggtg gaagcggccc
      841 agcacttcga aagcctggcc acgtttgcgt ccgaagccta tcgagtgctc agacctggag
      901 gcaggcttgc cgtcgcgacc ttcttcatga cccacgcgag cgccgccgat gagttgcggc
      961 agttgatcga aaccatcgac agtggcatcg acgtcgtcgt cgcgatcggc tcctttcgag
     1021 acgatctact caatgcaggc tttgccgaag ttgatgttga cagcatcggt gagcatgtct
     1081 ggtgcggttt cgacgcctgg atggagcaga ccgagttcaa agacagctgg ggccgcaact
     1141 ggctgaaggc ataccagcga gggcttatgg attattatct cgtcaccgca gacaagaagt
     1201 gatatctcat gatcaaattt ggacggtgct ggcgtggcgg catggcgtgc ggtgacggat
     1261 tcggagcccg acttcgccca gagagcacag acgctgcggg ccgacggctc gccgcggatc
     1321 agcgggatcg aggcgtcgtt cgtgcggggt cagctgtggt tcggctcgat gccggacgcc
     1381 cgcaagggtc gggacctgcg gcgggatccg cggttcgcgc tgaattttcg cgcggacatc
     1441 gacgaggttg tgatcactag ggtcggtgag ccggtggacc acctggtgat cgagctgtgg
     1501 cctccggatc gaccgctgca tcgcattcag cgccactgaa cggcgcttaa cgacgcaata
     1561 gggcgcagaa caacgcatca gtgccgtgcc ggtgcggcca caactggaca tgtggcccgt
     1621 cgccgagtcg tggcacgtcc gaaaactgct gcctggcgtc gagttgctcg acaccagtac
     1681 gccgcaccac gtcggcgacc acacccacgg tctcatcacg gtgcggcgag cacgtcacgt
     1741 aggccaccac accgccggga cggaccagcc gcagcgccgc cagcagcagc tcacgctgca
     1801 gccggaccaa cccggcgagg tcatcagcgc gccgccgcca acgcgcctcc gggcgccggc
     1861 gcagcgcccc gagcccggtg cacggtacgt ctaccagcac ccggtcgtag gaccgttccg
     1921 gcaattggct gttgcggccg tcggcgacgt gcaccgtcac cggcaggccc acgcaagcct
     1981 ggccgaccaa ccgggcccgg tgctcagctt tctccacagc gtccaggctg gccccgcgag
     2041 cggcagccag ggcacccagc aacgctgcct tgcctcccgg cccagcgcac aggtccagcc
     2101 agcgcacgtc agcgccctcc agctgaaccc cggccagcgc gagggcgacg agctggctgc
     2161 cctcgtcctg cacgtgggcc aggtgatcgc gcaccgccgc cagctcaccc gggtcaccgc
     2221 catcggcgag gtgcaccccg taaggcgagt acggggcggc ttcaccgccg gtcaccgcgg
     2281 ccaggtcggt agcggtgatc cggcccgggc gcgccgcgag atgcaccagc gggcgggcat
     2341 catcggcagc cagcgcttcg gtcagctcgt cggagtcccc gcccagcgcg tcggagaagg
     2401 cctgtgcgat ccaccgcggg tgcgggtggg ccatcgcaag gtagcccacc aggtcatcgc
     2461 cggctgccgg cgccactgcc tgcacccaac cttgctcgtc gtgctggctg acccggcgca
     2521 acacagcgtt gacgaagccg gcgctcgcac tgccgcgatc cgcacgcacc aggtcaacgg
     2581 tggtgcccac ggcggcgtgc gacggtaccc gggtccgcag cagctggtag ctgcccaacc
     2641 gcagggcatc cagcagttcg ccgtccactt ccgacagggg acgatcggtg caggactcca
     2701 acaccgcatc cagcaggccc tgcgcgcggc aggttccata gcacagctcg gtggccagcg
     2761 cggcgtcccg gccactgatc ccgcgctgcc ggagcaggcc aggcagcacc aggttgacgt
     2821 aggcgtcgcg ctcgcggacc gcagccagag cgtccagcgc ggcgcgccga gggaggtcct
     2881 ccaccggtgg ccggcgcggc ccggagcgcc gccggggcgg ccccggcctg gacggtcggg
     2941 acggcttgcg aggggccttc atggcggacc ctcgacgacc tgggcgatcc ccacactggc
     3001 tgtcactgga atcgctcccc cggcgccagc cgggcaccac gggcccagtc cggtgcgggc
     3061 atcgcccgct taccgggcgc agtgacctca cccagccgca ccgcgctgct c
//
LOCUS       DATADN010000579         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1133530, whole genome shotgun sequence.
ACCESSION   DATADN010000579 DATADN010000000
VERSION     DATADN010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133530"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VHH53_07480"
     CDS             <1..662
                     /locus_tag="VHH53_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX2290021.1"
                     /translation="SGLRGSKEFVRRAIEAGDRVVGALNNDMVGWANDDRLDNTIRYA
                     NPGLRDVQHAAALGFSDLITYDARYYKFTDAHSLVDGFGDVVSGIGSYPVLGNPHYHQ
                     PHDVLETVNHRLVAEVGRTTLATAVLMASSPSRLTGLMATRGADGSVDVRWNAAVERG
                     VTGYRVRWEDAEGKTGGMRVVKGPTARLTAVPQGATVQVRAIGSRGLEGWDWARAPLA
                     D"
     gene            complement(666..1244)
                     /gene="dcd"
                     /locus_tag="VHH53_07485"
     CDS             complement(666..1244)
                     /gene="dcd"
                     /locus_tag="VHH53_07485"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX2290022.1"
                     /translation="MSIKSDRWIRRMAKEHGMIEPFTERQVRQTDAQGRRVISYGVSS
                     YGYDMRVAPEFKIFTNVLSAVVDPKEFDARSFVEFEGPVCIVPPNSFALARSVEYFRI
                     PRNVLTICVGKSTYARCGIITNVTPFEPEWEGHVTLEISNTTPLPARVYADEGICQVL
                     FFEADADDVCETSYADKAGKYQGQRGVTLPRL"
BASE COUNT          235 a          504 c          530 g          242 t
ORIGIN      
        1 agtcggggct ccgcgggagc aaggagttcg tccgccgcgc catcgaggcc ggggaccgcg
       61 tcgtcggcgc gctcaacaac gacatggtgg gctgggcgaa cgacgaccgg ctggacaaca
      121 ccatccgcta cgccaacccc gggctgcggg acgtccagca cgccgccgcc ctcggcttca
      181 gcgacctgat cacctacgat gcgcgctact acaagttcac cgatgcccac tcgctggtgg
      241 acgggttcgg tgacgtggtc tcgggcatcg ggtcctaccc ggtcctggga aacccccact
      301 accaccagcc gcacgacgta ctggagaccg tgaatcaccg gttggtggcg gaggtgggcc
      361 gcaccaccct ggccacggcc gtcctgatgg cctcgagtcc gagccggctc accgggctca
      421 tggcgacccg tggggcggac ggcagcgtgg acgtccggtg gaacgcggcg gtggagcgtg
      481 gggtgaccgg ctaccgggtc cgctgggagg acgccgaagg gaagaccggc gggatgcggg
      541 tggtgaaggg gccgacggcc cggctgaccg cggtgccgca gggcgcgacg gtccaggtcc
      601 gggcgatcgg gagccggggc ctggagggtt gggactgggc ccgggcgccg ctggccgact
      661 agaccctaga ggcggggcag cgtcaccccc cgttgcccct ggtacttccc ggctttgtcc
      721 gcgtagctcg tctcgcacac gtcgtccgcg tccgcctcga agaacagcac ctggcagatc
      781 ccctcgtcgg cgtacacccg ggccggcagg ggcgtggtgt tgctgatctc cagagtgacg
      841 tgcccctccc actccggctc gaagggcgtg acgttggtga tgatgccgca gcgggcgtag
      901 gtactcttcc cgacgcagat ggtcagcacg ttgcggggaa tccggaagta ctccaccgag
      961 cgcgccagcg cgaagctgtt ggggggcacg atgcagaccg gcccctcgaa ttccacgaag
     1021 ctgcgggcgt cgaactcctt cgggtccacc accgcgctca gaacgttggt gaagatcttg
     1081 aactccgggg cgacccgcat gtcgtacccg tagctcgaga ccccgtagga gatgacccgc
     1141 cggccctggg cgtcggtctg gcggacctgg cgctcggtga acggctcgat catcccatgt
     1201 tccttggcca tgcggcggat ccagcggtcg gacttgatgg acacgggcac tccaggctgg
     1261 ggacagcgag tgcgggcgac cgcccgccga aaggcggcta atataggggc cgcgggggag
     1321 gcccggcagt cccatctcgc ccgtggtgcg atggttgaat ccgacctcta acgtccttag
     1381 ttttgacgag atacgtcacc gccacccgct cggagcatca tgctgcagcc gtacgttccg
     1441 gttctcctgc tcatcggctt cgtgatcgcg aacgcgctca tgatgctggc ggtgtcccac
     1501 atcttctcca c
//
LOCUS       DATADN010000580         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_527098, whole genome shotgun sequence.
ACCESSION   DATADN010000580 DATADN010000000
VERSION     DATADN010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527098"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..846)
                     /locus_tag="VHH53_07490"
     CDS             complement(10..846)
                     /locus_tag="VHH53_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX2290023.1"
                     /translation="MTETAAAASPDPDESSPSGFQVRLSNFEGPFDLLLQLIFAHRLD
                     VTEVALHRVTDDFIAYTKAIGPQLELDETTAFLVIAATLLDLKAARLLPAGEVHDEED
                     LALLEVRDLLFARLLQYRAFKHVAEMFAELEAAALRSYPRAVALEDRYSELLPEVMIG
                     VDAPAFAQIAAAAFTPRPVPTVSTDHLHHAAVSVPEQIGNLMTLLESRGIGQWASFRD
                     LVADCAAPIEIVGRFLALLELYRARAVAFEQPEPLGVLQISWTGERPSHQHLAVGEDH
                     YG"
     gene            complement(855..1679)
                     /locus_tag="VHH53_07495"
                     /pseudo
     CDS             complement(855..1679)
                     /locus_tag="VHH53_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
BASE COUNT          313 a          607 c          631 g          322 t
ORIGIN      
        1 gggtcgtcgt tatccatagt ggtcttctcc caccgcgagg tgctggtgtg acggacgttc
       61 gccggtccac gaaatctgca gcacaccaag cggttcaggt tgttcgaatg ctaccgcccg
      121 ggcgcggtag agctcgagca gcgccaagaa gcgcccgacg atctctatcg gcgccgcaca
      181 gtcggccacc agatcgcgga aggacgccca ttggccgatc ccgcggctct ccagcagtgt
      241 catcaggtta ccgatctgct cgggcaccga caccgccgca tggtgcagat ggtcggtgct
      301 caccgtcggc accggccgcg gcgtgaacgc ggccgcggcg atctgcgcga aggccggtgc
      361 gtcgacgccg atcatcactt cgggcaacag ttcggagtag cggtcctcca acgcgacagc
      421 gcgcgggtag ctgcgcagcg ccgcggcctc caactcggcg aacatctcgg ccacatgttt
      481 gaacgcgcgg tattgaagca gccgggcgaa cagcaggtcg cgcacctcga gcagggcgag
      541 gtcctcttcg tcgtgcacct cgccggcggg cagcagccgg gcggctttca gatcgagcag
      601 cgtcgcggcg atcaccagga aagcggtggt ctcgtcgagt tccagctgag gaccgatcgc
      661 cttggtgtag gcgatgaagt cgtcggtcac ccggtgcagc gccacctcgg tgacgtcgag
      721 gcgatgggcg aagatcaact gcagcagcag gtcgaacggg ccctcgaaat tgctgagccg
      781 gacctggaag ccggacggcg aagattcgtc gggatccggg cttgccgctg ctgccgtctc
      841 cgtcaccggg tcattcacgc gccgaaccgg tcgatgacct cgcgagccag caatcgatag
      901 ccttgtgccc cagcggattt cggcgcccaa gtggtgatcg gctcaccggc gacgctggtc
      961 tcggggaaac gcacggtgcg ggtgatgacg gtgtcgaaca ccaggtcgcc gaagcgctcg
     1021 acaactcggg ccatcacttc ccgggagttc acggtgcgcg ggtcataacg ggtgatcagg
     1081 atgccgctga tcgacagctt cgggttcagc ctgtcgtgca ccttgtcgac ggtgtcggtg
     1141 agcagcgcca gcccgcgcag cgagaagaac tcgcactcgg tcgggatgat cacaccgtcg
     1201 ctgcacgcca atccgttcac ggtgagcagc cccagcgagg gctggcagtc gatcagcacg
     1261 tagtcgtagc gatccaacac cgggtacagc gcgcgggcca gcgactgctc gcggccgacc
     1321 tcgttgacca gctggatctc cgctgccgac aggtcgatgt tgctcggcac caggtcgagg
     1381 ttcttgacgc gcgtgttgat cagcacgtcg tcgatcgaca cccgcggttc gaccagcagg
     1441 ttgtgcacgg tgtgttcgag ttcgtagtgc ggcacgccta gcccggccga cagggcgccc
     1501 tgtggatcga gatccaccag cagcacccgg cggccgtact cggcgaggct ggcgcccagg
     1561 ttgatcgtcg acgtggtctt gccgaccccg cccttctggt tgcacatcgc gacaaccttc
     1621 gccggcccgt ggctcgtcct cggctgcggt tcgggaatgt ttcgcggtgg acggccggtc
     1681 aggctggttg cttcgggcgt accgccctgc cctgcgtcgt cgctcatgcc cgaccgtcgc
     1741 taatcaggca attcacaggc atggcggaca tccgggcaag tttaacggga ggaacgtgcc
     1801 aggtcagcca gacccgcggg gcaacttgag gcccgatcgg cccgcctcgc ggcttagggt
     1861 gacgtcatgg gtc
//
LOCUS       DATADN010000581         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_674370, whole genome shotgun sequence.
ACCESSION   DATADN010000581 DATADN010000000
VERSION     DATADN010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674370"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VHH53_07500"
     CDS             <1..498
                     /locus_tag="VHH53_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290024.1"
                     /translation="ELRALATLGYRDDARRAATVAQRHMDTAGRDEPGRFVFDHAELN
                     QHLAEAFLRLGDTSAARLHAGRSLELKTTGSGGWAAATVILARAHAAEHSTADATALA
                     KSVLDTVPPALLRETTRRRLTALNADLLSERRSGQASRDLHDRLQTLPAHIPAQRTSP
                     EPNGQ"
     gene            complement(609..2162)
                     /gene="dnaA"
                     /locus_tag="VHH53_07505"
     CDS             complement(609..2162)
                     /gene="dnaA"
                     /locus_tag="VHH53_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX2290025.1"
                     /translation="MSERQTDLGQVWNEVVRELSTGTLSPQQRAWMRVTRPIGLLDGT
                     ALLAAPSDFAKDAIERVLRQPISAALSRHLGRQVSLAVKVDHPLPVPAELPLAAWAAD
                     GTNRPHAGDHRAAVPGSSDPVTAQVDPAEQRTNSSEQSEEDAALATVHEIWPSMAGAE
                     DAPAAAPTRRSSSQTKLNEKYTFDTFVIGASNRFAHAAAVAVAEAPARAYNPLFVWGE
                     SGLGKTHLLHAVGHYAQRLFPGMRVRYVSTEEFTNDFINSLRDDRKVAFQRRYRDVDV
                     LLVDDIQFLEGKEGTQEEFFHTFNTLHNANKQIVISSDRPPKGLGTLEDRLRTRFEWG
                     LITDIQPPELETRIAILRKKAAGDKLAAPDEVLEFIAARIERNIRELEGALIRVTAFA
                     SLNRQPVDNQLAEIVLRDLIPDDQAHEIGAPNIMAVTAEFFGVTIEDLCGPGKTKHLA
                     QARQISMYLCRELTDLSLPRIGQTFGGRDHTTVMHADRKIRKEMAERRRTYDQVQELT
                     SRIKQRARS"
     gene            2707..2850
                     /gene="rpmH"
                     /locus_tag="VHH53_07510"
     CDS             2707..2850
                     /gene="rpmH"
                     /locus_tag="VHH53_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX2290026.1"
                     /translation="MTKGKRTFQPNNRRRARTHGFRLRMRTRAGRAIMAARRRKGRTE
                     LSA"
     gene            2882..3253
                     /gene="rnpA"
                     /locus_tag="VHH53_07515"
     CDS             2882..3253
                     /gene="rnpA"
                     /locus_tag="VHH53_07515"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX2290027.1"
                     /translation="MLPAAARLTRRDDFATAVKRGRRAACGSVVVHVARSGDADAPAR
                     VGFVVGRTVGGAVVRHRVQRRLRHLMRCRLDALPGGALVVVRALPPSAGASSEALGDD
                     LSAALRRLTVSAATPNSGGRR"
     gene            3250..3552
                     /gene="yidD"
                     /locus_tag="VHH53_07520"
     CDS             3250..3552
                     /gene="yidD"
                     /locus_tag="VHH53_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX2290028.1"
                     /translation="MTPMTRGTATTPGARPMARVLVVLLQFYRQWISPFLPPSCRFHP
                     TCSAYAVEALTVHGALRGSWLTARRLLRCGPWHPGGMDPVPPPRSPRSPAESESSC"
BASE COUNT          529 a         1225 c         1195 g          605 t
ORIGIN      
        1 gaactccgcg cgctagccac ccttggctac cgcgacgatg cccggcgcgc ggccacggtc
       61 gctcaacgcc acatggacac tgccggccgc gacgaaccgg gacgcttcgt attcgaccac
      121 gccgagctca accagcatct cgccgaggca ttcctgcgcc tcggcgacac cagcgcggcc
      181 cgcttgcacg caggccgctc ccttgagctg aaaaccaccg gatcgggcgg ctgggccgcc
      241 gcgacggtga tcctcgcccg cgcccacgcg gccgaacaca gcaccgccga cgccaccgcg
      301 ctcgcgaaga gcgttctaga tacggtgcca ccggccttac ttcgggaaac cacgcgacgc
      361 cgcttgaccg ctttgaacgc cgacctgctc agcgaacggc ggtcggggca agcctcccga
      421 gacctgcacg accggctgca gaccctgccc gcacacatcc cagcacaacg caccagcccc
      481 gaacccaacg gtcaatgacc cggcgggtgc tatcagcgcg gtgtcgctgt ggcctaggtg
      541 gccgacgaga atatggccgt ggtcccctca ccaggcgctg ctgtcgtcgt aacccgtcca
      601 ggccgtggtc aggagcgggc gcgctgcttg atccgggagg tcagttcctg cacctggtcg
      661 taggtgcggc ggcgttcagc catctccttg cggatcttgc gatctgcgtg catcaccgtg
      721 gtgtggtcgc ggccaccgaa ggtctgcccg atccggggca gcgacaggtc ggtcagctcc
      781 cggcacaggt acatggagat ctgccgggcc tgggcgaggt gcttggtctt gccgggccca
      841 cacaggtcct cgatggtcac gccgaagaac tcagcggtca cggccatgat gttgggtgcg
      901 ccgatctcgt gtgcctggtc gtcggggatg agatcgcgca gcacgatctc ggcgagctgg
      961 ttgtcgacgg gttggcggtt cagcgacgcg aaggcggtga cccggatcag cgccccctcc
     1021 agctcacgga tgttgcgctc gatccgggcg gcgatgaact ccaagacctc atcaggcgcg
     1081 gccaacttgt cgccggcggc tttcttccgc aggatggcga tccgggtctc cagctcgggt
     1141 ggctggatat cggtgatcag gccccattcg aagcgggtgc gcaaccggtc ctccagggtg
     1201 cctagcccct tgggtggccg gtcggaggag atcacgatct gtttgttcgc gttgtgcagg
     1261 gtgttgaagg tgtggaagaa ctcctcctgg gtgccttcct tgccctccag gaactggatg
     1321 tcgtccacca gcagcacgtc gacgtcgcgg tagcggcgct ggaaggccac cttgcggtcg
     1381 tcgcgcagcg agttgatgaa gtcgttggtg aactcctcgg tggagacata gcgcacccgc
     1441 atgccgggga acagccgttg cgcgtagtgc cccaccgcgt gcagcaggtg cgtcttgccc
     1501 aaccccgact cgccccagac gaacagcggg ttgtaggccc gcgccggtgc ctcggccacc
     1561 gccaccgcag ctgcgtgcgc gaaccggttg gacgcgccga tgacaaaggt gtcgaaggtg
     1621 tacttctcat tgagcttggt ctgagaactc gaccgccggg tcggtgccgc agccggcgcg
     1681 tcctctgcgc ccgccatgct gggccagatc tcgtgcacgg tggccaacgc ggcgtcctcc
     1741 tcgctctgct cactggagtt ggttcgctgc tcggcgggat ccacttgtgc ggtgaccgga
     1801 tcggagctac cgggcaccgc ggcgcggtgg tcaccggcgt gcggccggtt ggtgccgtcg
     1861 gctgcccacg ccgccagcgg cagctccgcg ggtaccggta gtgggtggtc gaccttgacc
     1921 gccagcgaca cctgccgacc caggtgccgc gacagtgcgg cactgatcgg ctggcggagt
     1981 acccgctcga tggcgtcctt ggcgaagtcg ctgggtgccg ccagcagcgc cgttccgtcc
     2041 agcaggccga tcgggcgggt gacacgcatc cacgcgcgct gctgcggtga cagtgtgccg
     2101 gtggagagct ctcggacgac ctcgttccac acctggccga ggtcggtttg ccgctctgac
     2161 accgcgctga ctccccttcg gccggtggtg gactttcagg tcgtggactt caggaccgcc
     2221 gggtgtccac aaggttgtcc acagctgtgc atgaacggtt agcagcagcg atcggcgcac
     2281 tccccaaatc cccgacgtat cagtccgtgg accgtcctcc gcctgtcttc cggggccgca
     2341 agtgcaggca cgttgagcaa tctctgcctc tgccacaaga actccgggat agcaagcatc
     2401 accggagtaa cggcgtgtcg gccccgcagt gacgcgagac gctcgttttg acggcgccgg
     2461 tctcggccgc ataggctcgc gaggtctccc gcccaccggc gggtactctt gtgtgctctc
     2521 gccacgaccc gttggggttg cacgccgtgt gggacccgct cgaagtagtg ccgccggtgc
     2581 cggcaggcga tgactgaccg cagccccaca gctgcagtgc ctcacagctg cgaccctgca
     2641 ctgcgaccct gcactgcgac ctgcacaacg agataccgag caacgagcac cgggagaacg
     2701 acagccgtga ccaagggcaa gcgcacgttc cagccgaaca accgtcgtcg cgcgaggact
     2761 catgggttcc ggttgcgcat gcggacccgc gccgggcgcg ccatcatggc tgcgcggcgc
     2821 cgcaagggtc gcaccgaatt gtcggcctga aaccttattc cactctcgaa actttccttc
     2881 attgctgcct gctgccgccc gcctgacccg tcgcgacgac ttcgcgacgg cggtgaagcg
     2941 gggccgccgg gcggcgtgcg gctccgtcgt cgtgcatgtg gcgcgctccg gcgacgccga
     3001 tgcgccggcg cgggtgggct tcgtggtcgg tcggacggtt ggtggtgccg tggtccggca
     3061 tcgcgtgcag cgcaggctgc gccatctgat gcgatgccgg ttggatgcac tgcccggtgg
     3121 cgcactggtg gtggtgcgag cactgccgcc atcagccggc gcgagcagcg aggcgctggg
     3181 tgatgacctc agcgccgcgc ttcggcgact gaccgtttcc gccgcgacgc ccaactccgg
     3241 cggccggcga tgactccgat gacccgcggg accgcaacga ctccaggggc tcgcccgatg
     3301 gcccgggtgc tggtggtctt gctgcagttc taccgccagt ggatctctcc gttcctcccg
     3361 ccgagctgcc ggttccaccc cacgtgcagc gcgtatgcgg tcgaggcact gaccgttcac
     3421 ggggcactgc gtggatcatg gttgaccgcg cgacggctac tgcgctgcgg accctggcac
     3481 cctggaggga tggacccggt tcccccaccg agatcacccc gctcccccgc cgagagtgag
     3541 tcgtcgtgct gaac
//
LOCUS       DATADN010000582         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_674544, whole genome shotgun sequence.
ACCESSION   DATADN010000582 DATADN010000000
VERSION     DATADN010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674544"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VHH53_07525"
     CDS             complement(<1..588)
                     /locus_tag="VHH53_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2290029.1"
                     /translation="MLTLVDVGIGTRIRQFLLAPPTLLTVVMTVLAVLVNRDRLGLNL
                     AGGRLLPVEGLIETWSLYLASWHPVGGGSAAPPPAALAVIGLLGLPVGSPAAAVSLLL
                     LAQLPLAALSAYRATCALPVSRNRRALVAAGYALLPPAVAGLAQGRLDVVLVHLLLPL
                     VLAGSVAVLRGGTVGRSGGWLSTAAGTALGLAVIGA"
     gene            complement(819..1112)
                     /locus_tag="VHH53_07530"
     CDS             complement(819..1112)
                     /locus_tag="VHH53_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2290030.1"
                     /translation="MDLHQDRWAPIPQVPLVAGLFDEPDQQEWQERALCAQTDPEAFF
                     PEKGGSTREAKRICSGCEVRAECLEYALAFDERFGIWGGLSERERRRLKRRAV"
     gene            1384..>2107
                     /locus_tag="VHH53_07535"
     CDS             1384..>2107
                     /locus_tag="VHH53_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2290031.1"
                     /translation="MRATDLAPSPLFLLMVAATVAGGALAALTTGAARTAGVVLLVLG
                     GWAVALCLHEFGHALTAYRGGDRSVRAKGYLTLDIRRYTDPVMSLLLPLLLLAFGGIP
                     LPGGAVWINRNALRSRAIASAVSLAGPLTNLALGAVLITVISIGAFPDGLAAGLSYLA
                     LVQVLAFVLNILPVPGLDGFGAVEPYLPEQARHIANTVGLYAIVGLFLLIITVPAVAD
                     SLFGASFAVFAVLGGDPLAAAAG"
BASE COUNT          331 a          761 c          689 g          326 t
ORIGIN      
        1 ggcaccgatg acagcaagtc cgagcgcggt gccggccgcg gtggacagcc aaccgcccga
       61 tcgcccgaca gttccgccgc gcaggacggc gacggagccg gccagcacca ggggaagcag
      121 caggtgcacc agcacgacgt ccaaccggcc ctgcgccagc ccggccacgg ccggtggcag
      181 caacgcgtac ccggcggcga ccagtgcgcg ccgattccgg ctgaccggca gtgcgcaagt
      241 ggctcggtag gcggacagag cggccaacgg cagctgggcc agcagcagca gcgacacagc
      301 cgcggcaggt gatcccaccg gcaggcctag tagaccaatc accgccagtg cagccggcgg
      361 aggggccgcg ctcccgccac cgacgggatg ccaggaggcc aggtacaacg accaggtctc
      421 gatcaggccc tcgacgggca gcagccgacc tcccgccaag ttcagtccca gccgatcgcg
      481 gttcaccagc acggcgagca ctgtcatcac aacagtgagc agcgtgggag gggccagcag
      541 gaactgccgg atgcgggtgc cgatcccgac gtccaccaat gtcagcacgt ttgagtcgct
      601 gctgtccgcg gcgctgcggg cagtatcggg gtcggagggc ctgggcagtg gggagggccg
      661 gaggcgggtg cgcgtggcgg cgcctgaata cccctcctca ttcacaggag gttccccagc
      721 agtgcccgac acgccgaccc ctcctgtccg taccccgtta gtgcgggcac accgtacgtg
      781 tccggctcac catcacggac gccgggaggt gcccgcgctt agaccgcccg gcgtttaagc
      841 ctgcggcgct cgcgctccga cagcccaccc cagatgccga accgctcatc gaacgctaac
      901 gcgtactcca agcactcagc ccggacctcg caacctgagc agatccgctt tgcttccctg
      961 gttgagccgc ccttttcggg aaagaacgct tcgggatcgg tctgagcgca cagcgcccgc
     1021 tcctgccact cctgttggtc aggttcatcg aacaggccag caaccagcgg gacttggggg
     1081 atcggtgccc accggtcctg atgcaggtcc atgctgccgt ctggagagct gctgcagctg
     1141 ccgtccatct cgtccatggc ggtccgcctc ctcgccatag agttcctccc cagtccgcgc
     1201 cccgggctgc cgcgaacaat aacaagcctg tgattacacc ggtgtcatcg gggttgcaac
     1261 taccgtgagg cagcacgagc gcatcccccg gcgtgtcgcg attgacacgc gcagcaacct
     1321 tggcaaatca acggcaatcg ccccggtgga ttaaccggac ggcccatgag cgagggtgaa
     1381 gccgtgcgcg ccactgatct ggcccccagc ccgctgttcc tcctgatggt ggccgccacc
     1441 gtggccggcg gtgcgctggc ggcactgacc accggcgcgg cccgcaccgc tggcgtcgtg
     1501 ctgctggtgc tcggcggctg ggcggtagcg ctgtgcctgc acgagttcgg gcacgcgctg
     1561 accgcctacc ggggcggcga ccgttcagtg cgcgccaagg gttacctcac cctcgacatc
     1621 cggcgctaca ccgacccggt gatgtccctg ctgctgccct tgctgctgct ggccttcggc
     1681 gggatcccgc tacccggcgg cgcggtgtgg atcaatcgca acgcactgcg ctcgcgagca
     1741 atcgcctcgg cggtgtcgct ggccggcccg ttgaccaatc tggcgctggg cgccgtactg
     1801 atcacagtaa tcagcatcgg tgccttcccg gacgggctcg ccgccgggct gtcctacctg
     1861 gctttggtgc aggtgctcgc gttcgtgctc aacatcctcc cggtaccggg actggacggg
     1921 ttcggcgctg tggagcccta cctgcccgaa caggctcggc acatcgccaa caccgtcggc
     1981 ctgtatgcca tcgtcggcct cttcttattg atcatcaccg tgccggcggt ggccgactcg
     2041 ctgttcggcg ccagcttcgc ggtcttcgcc gtgctcggcg gcgacccact ggcagcagcc
     2101 gccggcc
//
LOCUS       DATADN010000583         3660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_674642, whole genome shotgun sequence.
ACCESSION   DATADN010000583 DATADN010000000
VERSION     DATADN010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674642"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1262
                     /locus_tag="VHH53_07540"
     CDS             45..1262
                     /locus_tag="VHH53_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2290032.1"
                     /translation="MLHPDGLLPPPAPDAAVGRGPSWLDGWLASYGADLVAWRRHIHA
                     HPELAFHEQGTTALVAEQLSSAGLRSTLLSGGTGLICDIGTGSRCVALRADIDALPLL
                     ESTGLPFASVVDGVMHACGHDAHTAILIGAALALASAPALPGRIRVIFQPAEEVMPGG
                     ALDVLAADGLSGVDRIFALHCDPRLPVGRFGTRVGPITSAGDMLELRLSSPGGHTSRP
                     HLTADLVHALGAVITGLPALLSRRVDPRSGTVLVWGAVQAGQAANTVPREGLLRGTLR
                     TGDREIWAELGPLVRELVTALLAPTGVGFDLRHVRGVPPVVNEPVSTRLLRDGVAAAL
                     GEDALSGTKQSSGGEDFGWYLEHVPGSMGRLGVWSGEGPQQELHHTDFELDERALPLG
                     VRIMVHTALAALT"
     gene            1446..2387
                     /locus_tag="VHH53_07545"
     CDS             1446..2387
                     /locus_tag="VHH53_07545"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX2290033.1"
                     /translation="MLTLPENPRAARTHAQLRAALGRRTLDRMRADGRVRELWGVLLP
                     RELLVDPDSRAAAALLAAGPAAALTGLTAAWLQGCPGAATPVVHVAVPYSRSLRTQQG
                     LVVHQGRRLLKEVIDVRGLRAVVLEVAIVELLCTDVARRALAVADQAAALLPERERLA
                     FRDRIADRLATRPDRRGTARAGGLLSLVTGRPDSAPESWLKLLVVEAGFPPPTEQFEV
                     TDALACVRYVLDLCWPELRIALEYDGYEAHEYRLDRDAERDLDLQRRGWTTIRATAED
                     LREPSALLARLDDAFRARGAAYFRAPLAEQRSRRRRA"
     gene            complement(2453..2905)
                     /locus_tag="VHH53_07550"
     CDS             complement(2453..2905)
                     /locus_tag="VHH53_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX2290034.1"
                     /translation="MPLYAAYGSNMDPAQMMQRAPHSPMAVTGWLIGWRLTFGGEDLG
                     WEGALATVVEDPLSQVFVVVYDVTPEDEKLLDRWEGGDFGFHKKLRLRVQTLDGPVLA
                     WLYVLDAYEGGLPSARYLGVIADAAEAAGAPEDYVTELRSRPCAGIGP"
     gene            3062..3301
                     /locus_tag="VHH53_07555"
     CDS             3062..3301
                     /locus_tag="VHH53_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290035.1"
                     /translation="MRIVACGLIAGAAMLGAAPAAMADEPAHDKARTSSDSQAAPEAE
                     VSMLAVSLTVGGAVATAGSGAALALGRRRKVSQPS"
     gene            complement(3285..>3660)
                     /locus_tag="VHH53_07560"
     CDS             complement(3285..>3660)
                     /locus_tag="VHH53_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX2290036.1"
                     /translation="HLARAVLEATAFQSREVLDAMNADSGVALASLKVDGGMVVNELL
                     MQFQADILAVPVIRPVVSETTALGAAYVAGLAVGFWSGEAEIRANWAADRQWNPAMEP
                     QQREELYTQWRKAVSRTFNWVD"
BASE COUNT          525 a         1240 c         1265 g          630 t
ORIGIN      
        1 gtgatcaccg tcagaggtgc cgtgtgactg cgctggaatc cgctttgctg catccagacg
       61 ggctgttgcc gccgcccgcg ccggacgctg ccgtcggtcg gggtccgtcc tggttggacg
      121 gttggctggc gagctacggc gccgatctgg tcgcatggcg caggcacatc cacgcgcacc
      181 cggaactcgc cttccacgag cagggcacca cggcattggt agccgagcag ctctccagtg
      241 ccggtctgcg gtccaccctg ctgtccgggg gcaccggcct gatttgtgat atcgggaccg
      301 gatcgcggtg cgtggcgctt cgcgccgata tcgacgcgct gcccctgttg gagagcaccg
      361 gtctgccgtt cgcgtccgta gtggacggtg tcatgcacgc ttgcgggcat gacgcgcata
      421 ccgcgattct gatcggcgca gcgctcgctc tggcatctgc cccggcgctg cccggccgga
      481 tccgggtgat cttccagccg gccgaggagg tgatgcccgg cggcgcactg gacgtgctcg
      541 ccgccgacgg gctgtccggt gtggatcgga tcttcgcact gcactgcgat ccacggttgc
      601 cggtcggccg gttcggaacc cgggtcgggc cgatcacctc ggcgggcgac atgctggagt
      661 tgcggctgtc atcgcccggt gggcacacgt cgcgcccgca cctcaccgct gatctggtgc
      721 acgcacttgg cgcggtgatc accggcctgc ctgcgttgct gtcccgacgg gtcgacccgc
      781 gttctggcac ggtgctggtc tggggtgcgg tgcaggccgg ccaggccgcg aacacggtgc
      841 cgcgggaggg gttgcttcgt ggcaccctgc gcactgggga tcgcgagatc tgggccgagc
      901 tggggccgtt ggtacgcgag ctcgtcaccg ctctgcttgc acccaccggg gtcgggttcg
      961 acctgcgaca tgtccgaggt gttccaccgg tggtcaacga gccggtcagc accaggctgc
     1021 tgcgcgacgg tgttgcggca gccctaggag aggacgctct gagcggaacc aagcagtcct
     1081 ccggtggtga ggacttcggt tggtatctcg aacatgtacc cgggtcaatg ggccgcctcg
     1141 gcgtctggtc cggtgagggc ccgcagcagg agttgcacca caccgacttc gagctcgacg
     1201 agcgcgcgct gccgctcggc gtgcgaatca tggtgcacac cgcactggct gccctcacct
     1261 aatccacctc ccggcgtgtc ctgctcccac gctcggtgtg tcgtgctcac ctgcgccgtg
     1321 tgtcgcgctg tggtgcgcgg tgtgtcgcgc gcccgtgccc ggctgctgtc cacaccgaag
     1381 cggtaatcca caggcctgtg cttcgccgct cgcagttcgg gcagccatcg agcgaggctc
     1441 acgccgtgct cacccttccg gaaaaccctc gcgccgcgcg gacacacgcc cagctacgcg
     1501 ccgctcttgg ccgtcgaacc ctggacagga tgcgggctga cggccgggtt cgggagctct
     1561 ggggcgtgct gctcccgcgg gagctcctgg tcgacccaga cagccgggca gccgcggctt
     1621 tgctcgcagc cggacctgcg gctgcgttga ccggtctgac ggcggcctgg ttacagggct
     1681 gcccgggcgc agcgacgccg gtcgtgcatg tggcggtgcc ctactcgcgc tcattgcgaa
     1741 cccagcaggg tttggtcgtt caccagggtc gaagactgct caaagaggtg atcgacgtgc
     1801 gaggtctgcg ggcagtggta ctcgaggtcg cgatcgttga gctgctgtgc accgatgtcg
     1861 ccaggcgcgc tctcgccgtc gccgaccagg cagccgcgct gttgcccgaa cgggaacggc
     1921 tagcatttcg cgaccggatt gccgatcggc tggccacccg gcccgatcgt cggggtaccg
     1981 cgcgtgctgg cgggttgctc tccttggtga caggccggcc cgactcggcg ccggagagct
     2041 ggctgaagct gttggtggtg gaggcgggat tcccgccgcc gacggaacag ttcgaggtca
     2101 ccgacgcgct ggcctgcgtc cggtatgtcc ttgacctgtg ctggccggag ttgcgcatcg
     2161 cgctggagta cgacggctat gaggctcacg agtatcggct ggaccgcgac gctgagcgtg
     2221 atctcgatct gcagcgccgg ggatggacca ccatccgcgc taccgcagaa gatctccgtg
     2281 agccgagcgc gctgctcgcc agactcgacg atgcttttcg cgccagggga gccgcttact
     2341 tcagggcgcc cctggctgaa cagcggtcac ggcgccgccg tgcctgagcg ctggacacac
     2401 cgtgcaccac agcgcgacac agcgtgcagg agcgccggac acgccggaga ggttaggggc
     2461 cgatgcctgc gcaggggcgg ctacgcagtt cggtgacgta gtcctccggg gcaccggctg
     2521 cttcggcggc atcagcgatg actccgaggt agcgcgccga ggggaggccg ccttcgtatg
     2581 cgtccagcac gtagagccag gcgagcaccg ggccgtccaa ggtctgcacc cgtagccgca
     2641 acttcttgtg aaacccgaag tcgccacctt cccagcggtc gagcagcttc tcgtcctccg
     2701 gagtgacgtc atagaccacc acgaagacct gagaaagcgg atcttcgacg acggtagcaa
     2761 gtgcgccctc ccagccgaga tcctcgcctc cgaaggtcag ccgccacccg atcagccagc
     2821 cggtcaccgc catcggcgag tgcggcgcgc gctgcatcat ctgtgcgggg tccatgttgg
     2881 acccataggc ggcgtagagc ggcacgcgcg acagcgtagc cgccagccgt gacatcgcag
     2941 cgctcccggc accgctatcc cttacgcgct gttcacagac ggccacggag cgtgctgcac
     3001 tcaggcaacc gccggccgtc ccatgcgtct tatcaggtac agcaagcctg aggaggttgc
     3061 gatgcggatc gtcgcctgcg ggctgatcgc gggtgcggcc atgctaggtg ctgccccggc
     3121 ggccatggcc gatgagccgg cccacgacaa ggcgcgcacc agttcggaca gccaggcggc
     3181 gccggaggcc gaggtgagca tgcttgcggt atcgctcact gttggcggtg cggtcgctac
     3241 ggctgggtct ggcgcagcgc tggcattggg ccgccgccgc aaggtcagtc aacccagttg
     3301 aaggtccggc tgaccgcctt gcgccactgc gtgtagagct cttcacgttg ctgcggttcc
     3361 atcgctgggt tccattgccg gtcggccgcc cagttagcgc ggatttcggc ctcgccggac
     3421 cagaatccca ccgcgaggcc ggcgacatag gcggcaccca gtgcggtggt ctcgctgacc
     3481 accggccgga tcaccggtac tgccaagatg tcggcctgga actgcatcag tagctcattg
     3541 accaccatcc cgccgtccac tttcaacgag gccagcgcca ccccggagtc ggcgttcatc
     3601 gcatcgagca cctcgcggga ctgaaacgct gtggcctcca acaccgccct ggccaggtgg
//
LOCUS       DATADN010000584         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_527537, whole genome shotgun sequence.
ACCESSION   DATADN010000584 DATADN010000000
VERSION     DATADN010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527537"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VHH53_07565"
     CDS             <1..371
                     /locus_tag="VHH53_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX2290037.1"
                     /translation="RSGSAEVPWLEPDVIDHLDALHVQGMRGVVVGPIGFVSDHLEVL
                     WDLDHEARQRAATLGMDFARAATPGTDPRFAEMVVELIAEHLQARPARRCSQVPAAGQ
                     GVNGQPCSPHCCHRHGDISG"
     gene            complement(457..1230)
                     /locus_tag="VHH53_07570"
     CDS             complement(457..1230)
                     /locus_tag="VHH53_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290038.1"
                     /translation="MTVWASAWLHGCAAPDAVIDGLHTWAGLHEVVAADEATARQLDL
                     PGPGEAPTSVVGLLAAARRAGAGGGRLLLPVPGDVRGLAPDPSLAAAALDSGEVAVLA
                     GPELAVVPNAVADGVLRWTVFAAGPLPPAPEPALSDAEHGLRGAVRQAATTLVELDVA
                     RHRPGVRAEIAEALERRVRPPWPDGTPGRALRVLEQADEVEAILGAAETDHLGGALSA
                     KVAAARSAALRPLSTAVREARRSAVAEAVRALTPRAGRR"
     gene            1340..>1964
                     /locus_tag="VHH53_07575"
     CDS             1340..>1964
                     /locus_tag="VHH53_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3097 family protein"
                     /protein_id="HEX2290039.1"
                     /translation="MPPDELQRSHRRPEVPQLIVDVGLVVEDAASGFCGAVRSCERDT
                     VALEDAQGRVRTFPLRCAAFLVDGDPVTLVRRAQPATAPARSASGSVRVAGLRARTAR
                     ASRIWVEGKHDAELVERVWGHDLRVDGVVVEALDGVDGLTKRVAEFCPAPHRRLGVLV
                     DHLVAGSKETRLVQAVRSEHVLVAGHPYVDIWQAVRPAALGIPSWPDV"
BASE COUNT          283 a          667 c          733 g          281 t
ORIGIN      
        1 cccgctccgg ttccgcagaa gtgccctggc ttgagccaga cgtgatcgac cacctcgacg
       61 cgctgcacgt ccagggaatg cgcggtgtcg tggtgggacc gatcggtttc gtctctgacc
      121 acctcgaggt gttatgggac ctcgatcacg aggctcggca gcgggctgcc acgctcggga
      181 tggatttcgc ccgagcggcc acaccgggca ccgatccgcg cttcgccgag atggtggtcg
      241 agctcatcgc cgagcatctg caggcacgac ccgcccgaag gtgctcgcag gtcccagcgg
      301 ccggtcaggg cgtcaacggt caaccctgct caccgcattg ctgccaccga catggagaca
      361 tatcggggta aagggagccc cagatacccc gatatgtctc catgtcggct tggcggtggg
      421 ggccggcttg gcggtgggtc ggtttggagt ggccggtcag cgccggccgg ctcgcggggt
      481 cagggctcgg accgcctccg cgaccgccga gcgccgggcc tcccgcaccg cggtggacag
      541 cggtcgtagc gccgccgagc gggcggccgc caccttggcg gacagcgcac cgccgaggtg
      601 atctgtctcc gcagcgccga ggatcgcctc cacctcgtcg gcttgctcga gtacccgtag
      661 cgcgcggccg ggggtgccgt cgggccacgg cggccggacc cggcgttcca gcgcttcggc
      721 gatctcggcg cggaccccgg gtcggtgccg ggcgacgtcg agctcaacca gtgtggtggc
      781 cgcctggcgc accgcaccac gcaagccgtg ctcagcgtcg gacagcgcgg gttctggagc
      841 cgggggtaac ggacccgcgg cgaacaccgt ccagcgcaac acaccgtcgg cgaccgcgtt
      901 gggaaccacg gccagttccg ggccggcgag cacggcgact tcgccactgt ccagcgccgc
      961 ggcagcaagc gatggatcgg gcgccagccc gcgcacatca ccgggcaccg gcaacagcag
     1021 acggccaccg cctgcaccgg cccggcgggc ggcggcaagc aggcccacca ccgaggtcgg
     1081 ggcctcaccg gggccaggta ggtcgagctg gcgtgcggtg gcctcgtcgg cggccaccac
     1141 ctcatgcagg cctgcccagg tgtgcaggcc gtcgatcaca gcgtcggggg cggcgcagcc
     1201 gtgcagccag gccgaggccc acacagtcaa tgtcgcactg ggacatggca cggagtagga
     1261 gggtagaacg ccgacgaaca acgcgcacac gcggcgcggc tgcaccgttg gattcaccca
     1321 tcgggttacc ttcggcgcca tgccccctga cgaactacag cggtcacatc ggcggcccga
     1381 ggtgccgcaa ctgatagttg acgtcggctt ggtggtggag gacgccgcga gcgggttctg
     1441 tggtgcggtg cgtagttgcg agcgcgacac ggtggccctg gaggacgctc aggggcgggt
     1501 gcggacattt ccgctgcggt gcgcggcgtt tctggtcgac ggtgatccgg tcaccctggt
     1561 tcgccgggcg cagccggcaa ctgcgccggc ccggtcggca tccggctcgg tccgggtggc
     1621 cgggctgcgg gcccggactg cgcgggccag ccggatctgg gtggagggca agcacgacgc
     1681 cgaactcgtg gaacgggtct ggggccacga tctgcgggtg gacggcgtcg tcgtggaggc
     1741 gctcgacggc gtggacggcc tgaccaaacg ggtcgcagaa ttctgtcctg caccacaccg
     1801 acggcttggt gtgctggtcg accacctggt ggccgggagc aaggagaccc gcctggtgca
     1861 ggcggtgcgt tcggagcacg tcctggtggc ggggcatccc tatgtcgaca tctggcaggc
     1921 tgtgcgccca gccgcactgg gcatccctag ctggccggac gtgc
//
LOCUS       DATADN010000585         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_674825, whole genome shotgun sequence.
ACCESSION   DATADN010000585 DATADN010000000
VERSION     DATADN010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674825"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VHH53_07580"
     CDS             <1..393
                     /locus_tag="VHH53_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290040.1"
                     /translation="RLYDNDVITAVITPNAMSPGPNGEAAVDFVLTSTLWWGKWINVP
                     DAEDTGANWTISTAAPWFGGRKTVDSVALWAHEVHNGQLLPFSKAKGLGIAWSVYQLG
                     GLERLAPHSRVTFTWGRGLTIYGRHCYR"
     gene            842..1696
                     /locus_tag="VHH53_07585"
     CDS             842..1696
                     /locus_tag="VHH53_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2290041.1"
                     /translation="MEQGEGDSVVAAFARASDALAAALDIQRAFWCERWPEGALLKLR
                     IALHTAEAQLRDEVNYFGPAVNRCARLRGVAHGGQVVLSKTTRDVVLDRLPEGVELVD
                     LGVHRLRSLGRPEHVFGLVHPELPVEFPPVGDDPGVGSGAPDVNWPLVGRADQLKRAS
                     TLLRTGRGAIVLAGAAGVGKTRLARECLGLAATRGFVTLRVAATQGAAELPFGAFASL
                     VPDLTLSADLLDVLRRIADAIVGRGRGNPVAVLVDDAHLLDQSSAALMHLLATTRAIH
                     KPQRSRGR"
BASE COUNT          280 a          502 c          602 g          350 t
ORIGIN      
        1 cggctgtacg acaatgacgt gatcaccgcc gtcatcacgc ccaacgcgat gagtccaggt
       61 ccgaacggcg aggccgccgt tgatttcgtc cttaccagca cgctctggtg gggcaagtgg
      121 atcaacgtac cagacgcaga ggacaccgga gccaactgga caatcagcac cgcagccccc
      181 tggttcggcg gccggaaaac tgttgacagc gtagcgctgt gggcccacga ggtgcacaac
      241 ggtcagctgc tccctttcag caaggcaaag ggccttggca tcgcttggtc tgtgtaccag
      301 ctcggtggac tggagcgctt ggcgccgcac tcgagggtga ccttcacatg gggtagagga
      361 ttaacgattt atggtcgcca ttgttatcgg tgaacgttct cgtcaaattt tcccaacaca
      421 ctagaaccgt tcatgccacc cgatcgcgac gcgtgggcgg cttctggcaa ttgaccgcat
      481 cggtgccctc ttcgctcaac accaatgtcg gcacgctaag acccgatcca cccccgttct
      541 gatatgaatc tctgcgaacc ggacccgttg gtcgtgtaca tgctggccgg ggcggtgcga
      601 cactgtcgtg agtgctcgga ctggttggtc aactcgccgc ggtgggggta tcgccagtgg
      661 acatgagcgg tatcgagcag gtgaccgttc atctgccggt gggcacggtg acgttcctgc
      721 tgacggacgt tgagggctcg acgttcctgt gggagtccgc cccggaggcg atgggtgctg
      781 catccgccgg cactaccagc tgctggatgc ggcgatcgcg cggcacggtg gggtgcgacc
      841 gatggagcag ggggagggcg acagcgtggt cgcggcgttt gcgcgtgctt cggacgcgtt
      901 ggcggccgcg ttggatatcc agcgtgcctt ttggtgcgag cgctggccgg agggtgcgtt
      961 gctgaagttg cgcattgcac tgcacaccgc ggaggcgcag ctgcgcgacg aggtcaatta
     1021 ctttggtccg gcggtgaacc gctgtgcccg gttgcgtggt gtggcgcacg gcggccaggt
     1081 ggtgctgtcg aaaacgacgc gggatgtcgt gctggaccgg cttcccgagg gtgtagagct
     1141 agtcgacctg ggtgtgcatc ggcttcgcag tcttggccgg ccggagcacg tgttcgggct
     1201 ggtacaccca gagttgcccg tggagtttcc ccctgtcggc gacgacccag gagtgggttc
     1261 gggtgcgccc gacgtcaact ggccgctcgt tgggcgggcg gaccagctga agcgagcgag
     1321 cacgctgctg cgtaccggtc gcggtgcgat agtcctagca ggagcggcag gggtggggaa
     1381 gacccggctg gccagggagt gccttggtct cgctgccacc cggggcttcg tgaccctcag
     1441 ggtcgcggcc acgcagggtg ctgcggagct tcccttcggg gcctttgcgt cgctggtgcc
     1501 cgacctgacg ttgagtgcgg acctgctgga cgtgctgcgc agaatcgccg atgcaatcgt
     1561 cggacgcggt cggggcaatc ctgtggctgt tctcgttgac gatgctcatc tgctcgatca
     1621 atcgtcggcc gctcttatgc atctactggc aaccacgagg gctattcaca agccgcaaag
     1681 aagtagaggc cgataacggc tttgagcatg ctttggtact tttctatcgg tgcc
//
LOCUS       DATADN010000586         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_212124, whole genome shotgun sequence.
ACCESSION   DATADN010000586 DATADN010000000
VERSION     DATADN010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212124"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VHH53_07590"
     CDS             complement(<1..431)
                     /locus_tag="VHH53_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX2290042.1"
                     /translation="MVFKVGETVVYPHHGAALIEAIETRVIKGEEKKYLVLKVAQGDL
                     TVRVPAANAEVVGVRDVVGQEGLDRVFEALRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRRERDRGLSAGEKRMLAKARQILVSELALAEG"
     gene            721..1338
                     /locus_tag="VHH53_07595"
     CDS             721..1338
                     /locus_tag="VHH53_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX2290043.1"
                     /translation="MSRWHRLMRRRLAVTALFAACCASAPSLVGCSAGQITQTDSQVA
                     AVDGTYADVGETIALRDVLIPAPPHSNGTYPAGSSVPVLLTIINQGTRADRLITVTSP
                     AASQVLVVGTTTIAPGTNVTSTAGSASAGVQPQSPLVVGQLRIVLSTTRPLRAGLNTP
                     VTFVFENAGTVTLPVPMAAPVESVSEVNVVESGEHGGDEHGGGHG"
     gene            1384..>2381
                     /locus_tag="VHH53_07600"
     CDS             1384..>2381
                     /locus_tag="VHH53_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2290044.1"
                     /translation="MARSPVHARAIHRCTECGHEVAKWVGRCPECQTWGSVVGAGPHP
                     VTRVAAGAPSSPARPISQVDLHSARATATGVSELDRVLGGGLVPGAVVLLAGEPGVGK
                     STLLLEVAHRCALAGRRSLYVTGEESAGQVRLRAQRTGALDDELFLAAESDLAAVLGH
                     LDAVHPDLLIVDSVQTMSCPGAEGSPGGVSQVRAVASVLITTAKQRGLPVVLVGHVTK
                     DGTVAGPRVLEHLVDVVLQFDGDRNSTLRMIRGIKNRFGPADEVGCFELTDDGITGLP
                     DPSGLFLTRRDGAVPGTAVTVVVEGKRPMLGEVQALVAASALANPRRAVSGLDSSRV"
BASE COUNT          369 a          821 c          804 g          387 t
ORIGIN      
        1 ccctcggcga gcgcgagctc actcaccaga atctggcggg ccttcgcgag catgcgtttc
       61 tcgccggccg acagaccgcg gtcacgctca cgacgccaca gatcccggac aacttccgcc
      121 actttgttga cgtcgccaga ggcgagcttc tccaagttgg ccttgtaccg ccgcgaccag
      181 ttcgtcggct cctcggtgtg gggagcacgc agcgcctcga aaacccggtc gaggccctcc
      241 tggccgacca cgtcccggac gccgacgact tcggcgttgg cagcgggaac acggacggta
      301 agatcaccct gagcaacctt gaggacgagg tacttcttct cctcgccctt gatcactcga
      361 gtttcgatgg cctcgatgag agcggcaccg tggtgcgggt agacgacggt ctctccgacc
      421 ttgaaaacca tgtgtcctct gcccctttcg ctacacctag gttaccacgc ctggaaaccg
      481 tcctgcgccg ggtatcacgg cgtcatcgca ggtcagagga tcggcaacca agtctcacca
      541 agcaacgccg cgctcccgca gctcaaggtg actccactgt agacctacac cagaccagac
      601 atgagccggc gccagaccgg caccgggtgg gtcggggcac tacggcgatg gatagaattc
      661 gccctggtcg gatgaggctg gccatcgatg cgcgagcaag acctgaggag gacttcgccc
      721 gtgagccgct ggcacaggtt gatgcggcgc cgcttggccg tcaccgccct gtttgccgcc
      781 tgctgcgcca gcgcgccgtc gctggtcggc tgcagcgcag gtcagatcac ccagaccgac
      841 agccaagtgg ccgctgtgga cgggacttac gctgacgtcg gggaaaccat tgcattgcgc
      901 gacgtgctga tccccgcccc gccccactcc aacggcacct acccggccgg ctcgagcgtg
      961 ccggtgctgc tcaccatcat caaccagggc actcgcgctg acaggctgat caccgtcacc
     1021 tcccccgcag ccagccaggt gctcgtggtg ggtaccacca cgatcgcccc cggaacgaac
     1081 gtcaccagca cggccggctc ggcttcagcc ggtgtccagc cgcagagtcc gctggtcgtc
     1141 gggcagctgc gcatcgtgct cagcaccaca cggccgctac gcgccgggtt gaacaccccc
     1201 gtgacattcg tgttcgagaa cgccggcacg gtgaccctgc cggtgcccat ggcggcgccc
     1261 gtggagtcgg tgagtgaggt caacgtcgtg gaaagcggcg agcacggcgg tgatgaacac
     1321 ggcggtgggc acggctaagt cggttcgccg cgctgtcgga ctggaccgat agcgtccgcg
     1381 accatggcaa ggtctccggt gcatgctcgc gccatccatc gctgcaccga atgcggccac
     1441 gaggtcgcca agtgggtagg gcgctgccca gagtgccaga cctggggcag cgtggtgggt
     1501 gccggtccgc atccggtgac gagggtcgcg gccggtgctc cgtcgtcccc ggcccgcccg
     1561 atctcccagg tcgacctgca ttcagcacga gccaccgcca ccggggtgtc cgaactcgac
     1621 cgggtactcg gcggtgggct ggtgcccggc gcggtggtcc tgctggccgg cgagcccggg
     1681 gtcggaaagt cgaccctgct gttggaggtc gcgcaccgct gcgcgctggc gggcagacga
     1741 tccctttacg tgaccggtga ggagtccgcg ggccaggtgc ggctgcgggc tcagcgcacc
     1801 ggcgcgctgg acgacgagct gttcctggct gccgagtccg acctcgccgc cgtgctcggg
     1861 catctcgacg cggtccatcc ggacctgttg atcgtcgatt cggtgcagac catgtcctgt
     1921 cccggagccg agggtagccc gggcggggtg tcccaggtcc gggcggttgc cagcgtgttg
     1981 atcaccaccg ccaagcagcg cgggctaccg gtggtgctgg tgggccacgt caccaaggac
     2041 ggcacggtgg cagggccccg ggtgctggaa cacctggtcg acgtcgttct gcagttcgac
     2101 ggcgaccgca actccacgct gcgcatgatc cgcggaatca agaaccggtt cgggccggct
     2161 gacgaggtcg gctgcttcga gctcaccgat gacggcatca ccggcttgcc cgatccgtcc
     2221 gggctgttcc tcacccgccg cgacggcgct gtgcccggga cggcggtgac ggtggtggtc
     2281 gagggtaagc ggccgatgct cggtgaggtc caggcactgg tcgcagcgtc cgcgctggcc
     2341 aacccgcgcc gggcggtcag cggtctggac tcttcccggg t
//
LOCUS       DATADN010000587         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1434469, whole genome shotgun sequence.
ACCESSION   DATADN010000587 DATADN010000000
VERSION     DATADN010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434469"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1432)
                     /locus_tag="VHH53_07605"
     CDS             complement(92..1432)
                     /locus_tag="VHH53_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding monooxygenase"
                     /protein_id="HEX2290045.1"
                     /translation="MRAVGHHAVVIGASMAGLLTARVLAEAYERVTVVERDVLPVCGE
                     HRRGVPQAHHLHGLLASGLAALETLFEGFAEEMVMAGALLGDVQADVRWYLDGHHYRQ
                     VPCGLHGLTASRPLLEGHVRARVAALPTVEVLDGCAAAGLEVSADGRRVTGLGVLARA
                     DGATAQVLDADLVVDASGRGSRTPVWLWQLGYPRPARDEVRIGVGYASRRYRLDSEVL
                     GGDGLGISNGTLTHRYGGGLQLLEHGQMLVTLGGYQGNHPPVDPEGFTAFAAQLPAGL
                     YEVIQQGEPLGDPIRFTFPVSVRNRYERLRRFPDGLLVLGDAMCSFNPIYGQGMSVAA
                     LEALALRDCLRGGTDRLAARFFPAAAKIVDNAWDISVTGDLRLPEVIGRRTLKVRLVN
                     AYLPHVHAAAAVDSRVGAAFLRVSNLLDAPPRLLSPTLMRRVLRTNLRRHRDHP"
BASE COUNT          328 a          607 c          535 g          242 t
ORIGIN      
        1 ctgaacttgc cgctgaacct gcccggagcg ttggagtcga cgagaaaggc ttaaagcgca
       61 attgggtacc cccactttct cattaccccc gtcatgggtg gtcgcggtgt cggcgcaggt
      121 tggtccgcaa cactcgccgc attagggtgg ggcttagcag gcgcggtggc gcatcgagga
      181 ggttggatac ccgcagaaat gcggcaccga cccgggagtc gaccgcggcg gccgcgtgta
      241 catgcgggag gtaggcgttg accaggcgca cctttagggt gcggcggcct atcacttcgg
      301 gcagcctgag gtcaccggtg acgctgatgt cccaggcgtt gtccacgatc ttcgcggccg
      361 cggggaaaaa ccgcgccgcc agccggtcag tgcccccgcg caggcagtcg cgtagcgcca
      421 gcgcttctag cgccgcgacg ctcatgccct gaccgtagat cggattgaag ctgcacatcg
      481 cgtcgcccag gaccagcaga ccatcgggaa atcgccgcag ccgctcatag cgattgcgca
      541 cgctgaccgg gaaggtgaag cggatcgggt caccgagcgg ttcgccctgc tggataacct
      601 cataaagacc agccggaagc tgggcggcga acgcggtgaa accctcggga tcaaccggcg
      661 ggtgattgcc ttgatacccg cccagggtga ccagcatctg cccgtgttcc agcagttgca
      721 agccaccgcc gtaacggtgg gtcagggtcc cattcgagat cccgagcccg tcgccaccga
      781 gcacctcaga atcaagccgg tagcgccgtg acgcgtaacc gaccccgatt cggacctcgt
      841 cgcgggccgg tcggggatag cccaactgcc acagccacac cggcgtgcgc gagcctcgac
      901 cggaggcgtc caccaccaag tcggcgtcta acacctgcgc ggtggcgcca tctgccctag
      961 ccagcacccc caggccggtc acccggcggc cgtcggcgct gacctcaagc ccggcggcgg
     1021 cacagccatc gagcacctcg acggtgggca gggcagccac ccgcgcccgg acgtggccct
     1081 ccagcagcgg ccggcttgcg gtcaggccgt gcagaccaca aggcacctgc cggtagtgat
     1141 gaccatcgag gtaccagcga acgtcggcct ggacgtcgcc taacagcgcg ccggccatca
     1201 ccatctcctc ggcgaacccc tcgaacaaag tctccagcgc cgccaggccg ctggccagca
     1261 gaccatgcag gtgatgggct tgcggaacgc cccggcgatg ctcaccgcac acgggcagga
     1321 cgtcgcgctc cacgacggtg acgcgctcgt aggcctcggc caacactctg gccgtgagca
     1381 gccccgccat gctggcacca atgaccactg cgtggtgccc gactgcgcgc atcgcggatc
     1441 ttcctgacac cttctaagcg aggtgcggta tcaattaaag ccttaccagg gcatcgctga
     1501 taccaccccg accagtgagc ctcggcgatc cggcccccac ccaggatgga gtggcgcgag
     1561 gcgtgagcca ccccacccac ccgcgccacc acaccaacca ctggtccacc tggcgacggg
     1621 tccacccgac acgcgcaaaa caaagccact accagcgaca acgcctcaaa cagtgaccgg
     1681 agacatcgta gagaccaagc atgggccttt tg
//
LOCUS       DATADN010000588         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1434655, whole genome shotgun sequence.
ACCESSION   DATADN010000588 DATADN010000000
VERSION     DATADN010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434655"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VHH53_07610"
     CDS             <1..288
                     /locus_tag="VHH53_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290046.1"
                     /translation="LNFLASEEHGRWTVKNLHTIPPRPMNTAGLDVSPLLAQVLDDTA
                     KVAAGGDFGYNIDVMVSDALNEAMYDGVQAVFTGQRTPEQVAASLETAAKK"
     gene            348..1289
                     /locus_tag="VHH53_07615"
     CDS             348..1289
                     /locus_tag="VHH53_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2290047.1"
                     /translation="MTTRRWPWRTIKAETPRVTRRAQARLGVFMVAPLAVMVAVFLLV
                     PMANAVYYVFVDFNGFDPNPPWIGFSNFTELAQDPDVWAAFKNNLIWIVIGTSAPIVF
                     GLGLAVLVWNIRRGSVLYRIAFFLPFILPQVAVGVVWGWIYEPSRGWLNRALEAVGLG
                     SFAKGWLGDPNTALYAVLGTAIWATVGFLFVIFLSALRNVDMDLVDAARLDGANGFQR
                     LRHIILPQIMPVFLMVTTITLVGGFSVFDIIFVMTGGGPAGATEVLGTYAYSSAFQLN
                     RISYGTTLALMITVISIPFAIWMNRLQRRLSLQGMGA"
     gene            1286..>1611
                     /locus_tag="VHH53_07620"
     CDS             1286..>1611
                     /locus_tag="VHH53_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290048.1"
                     /translation="MSYQTAGSGMRGRLLLTGKHALLLGLSALMLFPLVLTVSTALKT
                     GQDVKVNPFGLFSSFSTENIVTAWTVGDFSSYVLNSFLLSVPSTLLVVVLSTMAGYAF
                     ARLPFPG"
BASE COUNT          241 a          557 c          506 g          307 t
ORIGIN      
        1 ctgaacttcc tcgcgtccga ggagcacggc cgctggacgg tgaagaacct gcacaccatc
       61 cccccgaggc ccatgaacac cgccggcctc gatgtctcac ccctgttggc ccaggtgctc
      121 gacgacaccg ccaaggtcgc agcgggcggc gacttcggct acaacatcga cgtgatggtg
      181 tccgacgctc tcaacgaggc gatgtacgac ggcgtgcagg ctgtcttcac cggccagcgc
      241 acccctgagc aggtcgccgc gagtctcgag acagcggcca agaagtgacc gcaggcgctc
      301 ctgccgaggc gacagctccg gcgccggcaa cggcgccgga gcggggggtg acaacccgcc
      361 gctggccctg gcgaaccatc aaggcggaga ccccgcgggt cacacgccgg gcgcaggcgc
      421 ggctgggtgt gttcatggtg gctccgctgg ccgtcatggt ggcggttttc ctgctggtgc
      481 cgatggcgaa cgcggtgtat tacgtcttcg tcgacttcaa cggcttcgac cccaacccgc
      541 cgtggatcgg gttctcgaac ttcaccgagc tcgcgcagga cccggacgtc tgggccgcgt
      601 tcaagaacaa cctcatctgg atcgtcatcg gcacgtccgc tccgatcgtg ttcgggctgg
      661 gcctcgccgt gctggtctgg aacatccggc gaggcagcgt gctctaccgg atcgcgttct
      721 tcctgccgtt catcctgccg caggtggccg tcggtgtcgt ctggggctgg atctacgaac
      781 cctcccgcgg ctggctgaac cgcgccctcg aggcggtggg gctgggttcc ttcgccaagg
      841 gatggctcgg tgacccgaac accgcgctgt acgccgtgct cggcaccgcg atctgggcca
      901 ccgtcggctt cctcttcgtc atcttcctgt cggcgttgcg gaacgtcgac atggatctcg
      961 tcgacgccgc ccgcctggat ggggcgaacg gcttccagcg gctgcgccac atcattcttc
     1021 cccagatcat gccggtgttc ctcatggtca ccaccattac gctggtgggc ggtttcagcg
     1081 tcttcgacat catcttcgtc atgaccggcg gcggtccggc cggtgcgacc gaagtgctcg
     1141 ggacctacgc ctacagcagc gcgttccagc tcaatcggat ctcctacggc accacgctgg
     1201 cgctgatgat cacggtgata tccatcccgt tcgccatctg gatgaaccgg ctgcaacgca
     1261 ggctctcact ccaagggatg ggcgcatgag ctaccagacc gccggatccg gcatgcgagg
     1321 ccgcctgctg ctgaccggca agcacgctct cctgctcggc ctgtccgcgc tgatgctctt
     1381 ccctctggtg ctgacggtct ccacggcgct gaagaccggc caggatgtga aggtgaaccc
     1441 cttcggcctg ttctcgtcgt tctccaccga gaacatcgtg acggcctgga cggtcgggga
     1501 cttcagcagc tacgtcctca acagttttct gctgtcggta cccagcacgc tcctggtcgt
     1561 ggtgctctcc acgatggccg gatacgcgtt cgcgcgactg ccgttccccg g
//
LOCUS       DATADN010000589         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1265542, whole genome shotgun sequence.
ACCESSION   DATADN010000589 DATADN010000000
VERSION     DATADN010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265542"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="VHH53_07625"
     CDS             <1..1489
                     /locus_tag="VHH53_07625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX2290049.1"
                     /translation="EQLEFGLVREALHERELMLTRLAPHLVKPVSFLYPLAHRIWERP
                     YVAAGLALYQTLGGARSVPGQRHLTRAAALRMVPALRRDSLIGGVQYHDAITDDARHT
                     MTVARTAAHYGAVVRSSTQVIGFLREADRICGVRVRDCETGEETHVEARAVLNCTCVW
                     TDKIQRLSGGRGRFRVHPSKGVHIVVPRDRIASDSGLILRTATSVLFVIPWRNHWVIG
                     TTDTRWNLDLAHPAATRADIDYLLGTVNSVLATPLNGDDIEGVYAGLRPLLAGEREET
                     SRLSREHAVARVAPGLVAIAGGKYTTYRVMAADAVNAAVPDLPGRVAPSITDKVPLLG
                     ADGYHALVNQAETLAATHGLHPYRLRHLLDRYGSAVHQVLALAADRCDLLAPVRDAPD
                     YLRVEVVHAASHEGALHLEDVLTRRTRISIEYPHRGEACAADVADLMGEVLGWTVAAR
                     QREVAIYLARVAAERDSQRQFNDSTADARRGAAPEARPRLLEPVS"
     gene            complement(1592..1958)
                     /locus_tag="VHH53_07630"
                     /pseudo
     CDS             complement(1592..1958)
                     /locus_tag="VHH53_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133510.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          274 a          634 c          730 g          320 t
ORIGIN      
        1 ggagcagctc gagttcgggt tggtccggga ggccctgcac gagcgggagc tgatgctcac
       61 ccggctggct ccgcacctgg tcaagccggt gagcttcctg tacccgctgg cccaccggat
      121 ctgggagcgg ccttacgtcg ccgccgggct ggcgctgtac cagacgctgg gtggggcccg
      181 gtcggtgccg ggccagcgcc acctcacccg ggcggcggcg ctgcggatgg tgccggcgct
      241 gcggcgcgac agcctcatcg gtggggtgca gtaccacgac gcgatcaccg acgatgcccg
      301 gcacaccatg acggtcgccc gcacggccgc ccactacggc gccgtggtgc gcagttccac
      361 gcaggtcatc ggcttcttgc gggaggcaga ccggatctgc ggagtccgcg tgcgggactg
      421 tgagaccggc gaggagacgc acgtcgaagc gcgcgctgtg ctcaactgca cgtgtgtgtg
      481 gaccgacaag atccagcggc tgtccggtgg gcggggcagg ttccgcgtac atccctccaa
      541 gggagtgcac atcgtggtcc ccagggaccg tatcgcgtcc gacagcgggc tgatcctgcg
      601 caccgcgacg tcggtgctgt tcgtcatccc gtggcgcaac cactgggtca tcggcaccac
      661 cgacacccgc tggaacctcg acctcgcgca ccccgctgcc actcgcgcgg acatcgacta
      721 cttgctgggc accgtcaact cggtgctggc cacgccgctg aacggcgacg atatcgaggg
      781 tgtctacgcc gggttgcggc cgctgctggc gggtgagcgc gaggagacct cgcggttgtc
      841 ccgggagcat gcggtggccc gggtggcacc cggcctggtc gccatcgcag gcggcaagta
      901 caccacctac cgggtgatgg ctgccgacgc ggtgaacgcg gccgtgcccg atctgcccgg
      961 ccgggtcgct ccatcgatca cggacaaggt gccgctgctc ggcgcggatg gttaccacgc
     1021 actggtcaac caagctgaga cgctggccgc cacgcacgga ctgcacccgt accggctgcg
     1081 gcacctgctg gaccgctacg gctcagcggt gcaccaggtg ctggcgctgg ccgcggatcg
     1141 ctgcgacctg ctggcgccgg ttcgggacgc tccggactac ctcagggtgg aggtggtgca
     1201 cgccgccagc catgaggggg ccctgcacct ggaggacgtg ctgacccggc gcactcggat
     1261 ctcgatcgag tacccgcacc ggggcgaggc gtgtgctgct gacgtcgccg acctgatggg
     1321 tgaggtgttg ggttggacgg tggccgcgcg gcagcgggag gtcgcgatct acctggcacg
     1381 ggtggctgcc gagcgcgact cgcagcgcca gttcaacgat tccaccgccg acgcccgccg
     1441 tggcgccgcc cccgaggcgc gcccgcgcct tctggagccg gtgagctaac ccagccggcg
     1501 tgtcgtgctc tagtgcccgg cgtgtcaggc attggcgcac ggtgtgtccc gcgccattgc
     1561 tcagaattcg gcgtccatcg gcttgtggat actaagagcg accggcaaat gatcgttcgc
     1621 ggaggcgtcg ggtggtgggt ctggtgggcc agcggtaaag actgcgggct cgattgatca
     1681 ggcggcggat cacggtgagg gcggcggcga ggtagaggta gaactcggcg gtgatcttgc
     1741 gtttgtcggt gcaccggcgg agttgccgta gccgttcatc caggagtggg tgcgcttgac
     1801 cggccagcgt ttcccggcct ggatcggggc cggagtgccc tgggtggcga tgtcggcgtc
     1861 gtaacccaga atttcgagta ggtcgcgggt cgtggcggag tcgtagccgc ggtcaaggtg
     1921 taggcacggc tggtcgggca gtggtccgat catgtcgc
//
LOCUS       DATADN010000590         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1434736, whole genome shotgun sequence.
ACCESSION   DATADN010000590 DATADN010000000
VERSION     DATADN010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434736"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..941
                     /gene="tsf"
                     /locus_tag="VHH53_07635"
     CDS             126..941
                     /gene="tsf"
                     /locus_tag="VHH53_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX2290050.1"
                     /translation="MANYSAADVKRLRDLTGSGMMDCKKALEESGGDFDQAVELLRIK
                     GAKDVGKRAERTTANGLVAAKGGVMIELNCETDFVAKGADFQELADKIVAEAMQIRPS
                     DPETLAQAELNGGTVADAVQTVSAKIGEKLQLRRVVSFDGEVATYLHRRASDLPPAVG
                     VLVEYHGGSPETARAVAMQVAAMKPRYVTREDVPADVLASERRIAEETARQEGKPEQA
                     LPRIVEGRLNGFFKDTVLLEQSSVQDSKKTVKAVLDDAGVTVQRFARFEVGQA"
     gene            960..1697
                     /gene="pyrH"
                     /locus_tag="VHH53_07640"
     CDS             960..1697
                     /gene="pyrH"
                     /locus_tag="VHH53_07640"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX2290051.1"
                     /translation="MNTDRLLGPGYRRVVLKLGGEMFGGGGVGVDPEVVRAVARQIAD
                     VVATGVQMAVVIGGGNFFRGEELQEGGMERSRADYMGMLGTVMNCLALQDFLEREHKL
                     DTRVQTAITMGQVAEPYIPRRAMRHLEKGRVVIFGAGVGMPYFSTDTTGAQRALEIGC
                     EVLLLGKAVDGVYTADPRTDPSATMFRHITHREVLERGLKVADATAFSLCMDNKLPII
                     VFNLLTEGNIARAVRGEPIGTLVSSPT"
     gene            1799..>2052
                     /locus_tag="VHH53_07645"
     CDS             1799..>2052
                     /locus_tag="VHH53_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX2290052.1"
                     /translation="MIDDTLFDAEEKMDKAVSVAKEDLATVRTGRASPAMFSKIVVDY
                     YGATTPLIQLASVTVPEARMAVIKPYDPSLLRPMAKAIRDS"
BASE COUNT          386 a          627 c          718 g          321 t
ORIGIN      
        1 ctcggcagag cggtcagcgg caccgcaggc agaaccggcg actgcgaaca acggcgtgtc
       61 gcatacctga gaccgcaccc ttcgacccga agaacgagac aagaacgaca ggacggaaag
      121 ccacgatggc gaactactcc gccgcggatg tcaagcgact ccgggacctg accggttccg
      181 ggatgatgga ttgcaagaag gccctggaag agtccggcgg tgacttcgac caggcggttg
      241 agctcctgcg catcaagggt gccaaggacg ttggcaagcg ggccgagcgc accaccgcca
      301 acgggctggt ggccgccaag ggcggcgtga tgatcgagct caactgcgag accgacttcg
      361 tggccaaggg tgccgatttc caggagctcg ccgacaagat cgtcgctgag gcgatgcaga
      421 tccgcccgtc cgaccccgaa acactcgcgc aggccgagtt gaacggcggc acggtggccg
      481 atgcggtgca gaccgtctcg gccaagatcg gcgagaagct gcagctgcgc cgggtggtgt
      541 ccttcgacgg tgaggtggcc acctacctgc accgtcgcgc ttcggacctg ccgcctgcgg
      601 tgggcgtgct ggtcgagtac cacggtgggt cacctgagac ggcgcgtgcc gtggcgatgc
      661 aggtggctgc gatgaaaccg cgctacgtca cccgggaaga cgtgccggcc gacgtgctgg
      721 ccagcgagcg gcgcatcgcc gaggagaccg cgcgccagga gggcaagccc gagcaggccc
      781 tgccccggat cgtcgaaggc cgtctcaacg gcttcttcaa ggacactgtg ttactggagc
      841 agtcctccgt gcaggattcc aagaagaccg tcaaggctgt cctggatgac gccggagtga
      901 cggtgcaacg gttcgcccgg ttcgaggtcg gccaggcctg aggcaggatg aacccgccga
      961 tgaacaccga ccggttgctg ggccctgggt accgccgggt ggtgctcaag ctcggcggtg
     1021 agatgttcgg cggtggcggc gtcggggtgg atcccgaggt ggtacgcgcc gtcgcgcggc
     1081 agatcgccga cgtggtcgcc accggggtcc agatggcggt ggtgatcggc ggagggaact
     1141 tcttccgcgg cgaagagctg caggaaggcg gtatggaacg gtcccgggct gactacatgg
     1201 gaatgctcgg gaccgtcatg aactgcctgg ctttgcagga tttcctggag cgtgagcaca
     1261 aactcgacac ccgcgtgcag accgcgatca ccatggggca ggtcgccgag ccctatatcc
     1321 cgcggcgggc catgaggcac ctcgagaaag gccgtgtggt gatcttcggc gcgggtgtcg
     1381 ggatgcccta tttctccacc gacaccaccg gggcgcaacg cgcgctggag atcggctgtg
     1441 aggttctgct gttgggcaaa gcggtcgacg gcgtgtacac cgctgatccc agaaccgatc
     1501 cctcggccac gatgtttcgc cacatcactc atcgtgaggt actggagcgc gggctcaagg
     1561 tggccgatgc gaccgcattt agcctgtgca tggacaacaa gttaccgatc atcgtgttca
     1621 atctgctcac cgaaggcaac atcgcgcgtg cggtgcgcgg cgaaccgatc gggaccctgg
     1681 tcagttcccc gacgtgagat ccgccggtcg ggtggttccg cagggaagcg acccagggga
     1741 gatactggta caggccacgc cgcccaggcg ccagccggat gccggacaag gagcagccgt
     1801 gatcgacgac actctcttcg acgccgagga gaagatggac aaggccgtgt cggtcgccaa
     1861 ggaagacctg gccacggtgc gtactggccg agccagtccg gcgatgttct ccaagatcgt
     1921 cgtggactac tacggcgcca ctactccatt gattcagctg gccagcgtga cggtcccgga
     1981 ggccagaatg gctgtgatca agccgtacga tcccagcctg ctgcggccca tggcgaaggc
     2041 gatccgggat tc
//
LOCUS       DATADN010000591         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_382223, whole genome shotgun sequence.
ACCESSION   DATADN010000591 DATADN010000000
VERSION     DATADN010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382223"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VHH53_07650"
     CDS             complement(<1..261)
                     /locus_tag="VHH53_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEX2290053.1"
                     /translation="MQRRIFGIETEFGVTCTFHGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLRNGSRLYLDVGSHPEYATAECDDLAQLVTHDKAGERILE"
     gene            329..1819
                     /locus_tag="VHH53_07655"
     CDS             329..1819
                     /locus_tag="VHH53_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2290054.1"
                     /translation="MLQWVRRAAQKINQADRELVRRSAAIPPSAADEGLKRLTRTANH
                     SLLWFAAAAVLAGRKGVTRRAAFRGVAAIGGASLTINAVAKPLMPRRRPAWEDVLVPR
                     RLHRHERPSSSSFPSGHAASAAAFTTAVTMECPAVGLAVAPVAAVVAYSRLHTGVHYP
                     SDVIAGMLLGSGVALATRRWWPLRVGEAAASSHPAPAPALPDGEDLVVLINAGSGADG
                     GHPGTELAAVWPAARQVVIEPGGDLGKQLEAELDASDRPPARAVAVAGGDGTVAAVAA
                     VAASRGLPLVVVPAGTLNHFARDIGVTGLPDAIQAVRAGSAMAVDLGGVQVDDAPPHW
                     FLNTASLGGYPDLVQLRERREARWGKWPSAALAMIRVLREADPLVARIDGKPRKAWLV
                     FVGNGLYRPRGFAAAARHRLDSGLFDVRYVRADVPLSRTRFLVGALSSALERSRTYVQ
                     QERAEMLIEVLGPPVALATDGEVITEGRRFRFFTQPAALQVYRPTR"
BASE COUNT          271 a          671 c          724 g          313 t
ORIGIN      
        1 ctcgaggatc cgctcgccgg ccttgtcgtg ggtcaccagc tgggccaggt cgtcgcactc
       61 cgcggtggcg tactccggat gtgagcccac gtcgaggtac aaccgggagc cgttgcgcaa
      121 gaacacgttc gacgagcgac cccacgagac cacccggcgg aacaggtagc gggcgacctc
      181 gtccggcgac agccggcgct gcccgtggaa ggtgcaggtg accccgaact cagtctcgat
      241 gccgaagatc cgccgctgca tgccttccag cttaggctgc cgaggccgtc ctgcaggccg
      301 tccgtacggg ctcacttacg ctgcacgggt gctgcaatgg gtgcgccgag cggcgcagaa
      361 gatcaaccag gccgaccgcg agctggtgcg ccgcagtgcg gccatcccac cctcagctgc
      421 cgacgagggt ctcaagcggc tcaccagaac cgccaaccac agcctgctgt ggttcgctgc
      481 ggctgcggtg ctggccggcc gcaagggtgt gacccggcgc gccgcatttc gcggcgtggc
      541 ggcgatcggg ggggccagcc tcaccatcaa cgcagtggcc aaaccgctga tgccacgccg
      601 gcggccggcc tgggaagacg tcctcgtgcc acgccggttg catcgccacg aacggcccag
      661 ctcgtcgtcg ttcccctccg gtcacgcggc gtcggcggca gcgttcacca ccgcggtgac
      721 gatggaatgt cctgctgtgg ggcttgccgt ggccccggtt gcggcggtgg tggcctactc
      781 ccggctgcac accggcgtgc actatccctc cgacgtgatc gccgggatgc tgctgggcag
      841 cggcgtcgcg ctggccaccc ggcgatggtg gccgttgcgg gtcggcgaag cggccgcgag
      901 tagccacccg gcgccggcgc cggcgctgcc tgatggtgag gatctggtgg tactgatcaa
      961 tgccggttct ggagccgacg gcgggcaccc cggtaccgag ctggccgccg tctggcccgc
     1021 agcccggcag gtggtcatcg aacccggtgg cgatctaggc aagcagctgg aggcagagct
     1081 ggacgcctcc gaccgaccgc cggcgcgtgc agtggcggtc gcaggcggcg acgggacggt
     1141 ggcggcggtg gcggcggtgg cagccagccg ggggttgccc ctggtggtgg tgccggccgg
     1201 cacgctgaac cacttcgcac gggacatcgg ggtaaccgga ttgcccgacg ccatccaagc
     1261 ggtgcgcgct ggcagcgcca tggcggtgga cctgggtgga gtgcaggttg acgatgcgcc
     1321 gccgcactgg ttcctcaaca ccgccagcct cggcgggtat cccgacctgg tgcagctgag
     1381 ggagcgcagg gaagcgcgct ggggcaagtg gccatcggcg gcgctcgcaa tgatccgggt
     1441 gctacgcgag gcagatccgt tggtggcgcg catcgacggt aagccgcgca aggcgtggct
     1501 ggtctttgtg ggcaacggtc tgtatcgccc ccgcggcttc gctgccgcgg cccggcaccg
     1561 gctggattcc gggctgttcg acgtccgtta cgtccgtgct gacgtgccgc tgtctcggac
     1621 ccggttcctt gtcggggccc tgtccagcgc cctggagcga agccgaacct acgtccagca
     1681 ggagcgtgct gagatgctga tcgaggtcct cggcccaccg gtggcgttgg ccaccgacgg
     1741 cgaggtcatc acggagggac gccggttccg gttcttcacc caacccgctg cgttgcaggt
     1801 ttaccgcccg acgcgctgac cgttagctgg cggggctgga tccaccgttg gtgccgttgg
     1861 cgtcggtacc ctcgcccggc gtcgagtccg aggtatcccc cttgggttcg gtcgtcgagc
     1921 tgtcggtacc gggtaacaac gcggtcagtg ccgccccggt gatccgccgg aaagcgcgc
//
LOCUS       DATADN010000592         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_528672, whole genome shotgun sequence.
ACCESSION   DATADN010000592 DATADN010000000
VERSION     DATADN010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528672"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VHH53_07660"
     CDS             <1..741
                     /locus_tag="VHH53_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2290055.1"
                     /translation="GVCTLDRPQARNAMTPAMYFGIRYAVRHVDADRDLAGLLITGTG
                     DVFAPGGDMGGGGSTDNWLTFGSALGMDVTPFETLRQSVKPVVSAVNGLCQGGGLQIA
                     LCSDMAVVSDRATFRVPELFRGIADTYYSQMLTRLIGPVRTRDLMLTGRTLTAQEAYD
                     WGMVARIVPHDDLLGTAKEVLAQCCRTAPQARSVVKSSIDNYLGLFDRIGMQASYSSP
                     EAMEGYRSFKERRSPEWVHPELRVDGRL"
     gene            complement(775..>2546)
                     /locus_tag="VHH53_07665"
     CDS             complement(775..>2546)
                     /locus_tag="VHH53_07665"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX2290056.1"
                     /translation="VVGAPLRYQHRIYNTAVVIHRGQILGVVPKSYIPNYREFYEKRQ
                     IAAGDEERGEIRLGGEEVPFGSDLLSEATDLPGFVLHVEICEDMWVPVPPSAEAALAG
                     ATVLANLSGSPITIGRSEDRCLLARSASSRCLAAYVYAAAGQGESTTDLSWDGQTMIW
                     ENGLCLAQSERFPKGERRSVADVDLELLRNERLRMGTFDDNRRHQMIDEDSFRRIEFT
                     LDPPTGDIGLYREVERFPFVPADPARLEQDCYEAYNIQVAGLEQRLRALDYPKIVLGL
                     SGGLDSTHALIVAARAMDREERPRSDILAFTLPGFATGERTRNNATRLAEALGVTFET
                     IDIKSTAELMLKEMDHPFSRGEKVYDVTFENVQAGLRTDYLFRLANQRGGIVLGTGDL
                     SELALGWSTYGVGDQMSHYNVNGGVPKTLIQHLIRWVISSKQFDETVNDVLQSVLDTE
                     ISPELVPAGEDEEIQSSEGKVGPYVLQDFSLFQVLRYGFRPSKVAFLAWHAWSDAEHG
                     DWPTGFPDSKRPTYSLGEIRHWLQVFAQRFYSFSQFKRSALPNGPKVSAGGSLSPRGD
                     WRAPSDMSARVWLDEIENSIPTG"
BASE COUNT          415 a          874 c          843 g          414 t
ORIGIN      
        1 ggggtgtgca cgctcgaccg cccgcaggcg cgcaacgcga tgacgccggc gatgtacttc
       61 ggcatccggt atgcggtgcg ccacgtcgac gcggaccgcg atctggcggg gctgctgatc
      121 accggtaccg gtgacgtgtt cgcgccgggt ggagatatgg gtgggggcgg aagcaccgac
      181 aactggctga cgttcgggtc ggctctcggt atggatgtga cgccgttcga aaccctgcgc
      241 cagtcggtca aaccggtggt gtcggcggtc aacggcctgt gccagggcgg cggtctgcag
      301 atcgcgttgt gcagcgatat ggccgtggtc agcgatcgcg ccaccttccg ggtgcccgaa
      361 ctgtttcgcg gcatcgccga cacctactac agccagatgc tgacccgcct catcggtccc
      421 gtccgtaccc gcgacctgat gctgaccggc cggacgctga ccgctcagga ggcgtacgac
      481 tggggcatgg tggcgcggat cgtcccgcac gacgacctgc tcggcaccgc gaaagaggtg
      541 ctcgcgcaat gctgtcgcac cgcaccgcag gcgcgcagcg tggtcaagtc cagcatcgac
      601 aactacctcg ggctgttcga ccggatcggc atgcaagcca gctattcgtc gccggaggcg
      661 atggagggct accgctcctt caaggagcgg cgctcaccgg aatgggtgca tccggagctg
      721 cgggtcgacg ggcggctgta gcggccgcac tcatgggcgc tgagctgtca acggtcagcc
      781 cgtcgggatg gagttctcga tctcgtcgag ccacacccgc gccgacatgt ccgacggcgc
      841 ccgccagtcg ccacgcgggg acagtgatcc cccggcagag accttgggcc cgttgggcag
      901 agccgaccgc ttgaactggc tgaacgagta gaaccgctgg gcgaacacct gcagccagtg
      961 cctgatctcc ccgagggaat aggtgggccg cttgctgtcc gggaacccgg tgggccagtc
     1021 cccgtgctcg gcgtcgctcc atgcgtgcca ggccaggaat gccaccttcg acgggcggaa
     1081 cccgtagcgc agcacctgga acagcgagaa atcctgcagg acatacggcc ccaccttgcc
     1141 ctcgctgctc tggatctcct cgtcctcacc ggcgggcacc agctccgggc tgatctcggt
     1201 gtcgagcacc gactgcagca cgtcgttgac cgtctcgtcg aactgcttcg acgagatgac
     1261 ccagcggatc aggtgctgga tcaacgtctt cggcacaccg ccgttgacgt tgtagtgcga
     1321 catctggtcg cccaccccgt aggtcgacca gcccagcgcc agctcggaca ggtcgccggt
     1381 gcccagcacg ataccgccgc gctgattggc aaggcggaaa aggtaatccg tgcgcagacc
     1441 ggcctgcacg ttctcgaacg tgacgtcgta gaccttctca ccgcgggaga acgggtggtc
     1501 catctccttg agcatcaact cggcggtcga cttgatgtcg atggtctcga acgtcacacc
     1561 gagcgcctcg gccaaccggg tcgcgttgtt cctcgtgcgc tcaccggtcg cgaaacccgg
     1621 cagcgtgaac gcgaggatgt cgctgcgcgg ccgttcctca cggtccatcg cccgcgccgc
     1681 cacgatcagt gcgtgcgtgg agtccagacc gcctgacaga ccgagcacga tcttcggata
     1741 gtccagtgcc cgcaaccgct gctcgagccc ggccacctgg atgttgtagg cctcgtaaca
     1801 atcctgttcc agccgtgcgg gatccgcggg cacgaacggg aaccgctcca cctcgcggta
     1861 cagcccgatg tccccggtcg gcgggtcgag ggtgaactcg atgcgccgga aggaatcctc
     1921 gtcgatcatc tgatggcgcc ggttgtcgtc gaaagtgccc atccgcagcc gctcgttgcg
     1981 cagcagttcg aggtccacgt cggcgaccga gcgccgctct cccttcggga accgctccga
     2041 ctgcgccagg cacaggccgt tctcccagat catcgtctgc ccgtcccagg acaggtcggt
     2101 cgtcgactcc ccctgccccg ccgcggcgta cacgtaggcg gccagacacc gcgacgacgc
     2161 cgaccgcgcc agcaggcacc ggtcctcgga ccggccgatc gtgatggggc tgcccgacag
     2221 gttggccagc accgtcgcac cggccagcgc ggcctcggcg ctgggcggca ccggcaccca
     2281 catgtcctcg cagatctcga cgtgcaggac gaagccgggc aggtcggtgg cctcggacag
     2341 cagatcggaa ccgaacggca cctcctcgcc gcccagccgg atctcgccgc gctcttcgtc
     2401 gcccgcggcg atctgacgct tctcgtagaa ctcgcgatag ttggggatgt aggacttcgg
     2461 caccacgccg aggatctgcc cgcggtggat cacgacggcg gtgttgtaga tccggtgctg
     2521 gtagcgcagg ggggcgccga cgacca
//
LOCUS       DATADN010000593         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_213355, whole genome shotgun sequence.
ACCESSION   DATADN010000593 DATADN010000000
VERSION     DATADN010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213355"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1137)
                     /locus_tag="VHH53_07670"
     CDS             complement(259..1137)
                     /locus_tag="VHH53_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX2290057.1"
                     /translation="MTRHVADRIAAGLGGLAPAQRRVARFFTEHATQLGFFSAAEIAA
                     RLGTSDATVVRTAQTLGYRGLTDLKRALLDDAPPPEPTPSARLGATLRHATAPRDVLE
                     HVLDVHRAALNAARNSLDEQFPAAVALLAAAERIVLSGTGPSAAVAQYAAVLLGRVGR
                     PATTITATGVSMADQAMGLRRGDALLLLAYTRLHTHAAVLIGLARRRRIPVLLVTDVL
                     ADVDGADLVLSCPRGMPGEQSSHAVTLLLLEATAVALAATGPARATTALSELNRLRGE
                     LLGVPADVDTPDADPR"
     gene            1185..1931
                     /locus_tag="VHH53_07675"
     CDS             1185..1931
                     /locus_tag="VHH53_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290058.1"
                     /translation="MLDLVAVALPAFLASLVEFVEAFTIVLAVGTSRNWRAALAGAAT
                     ATVALAALLIAFGPGLLERVDEHVLLLVIGILLLLFGGRWLCKAMLRSAGLIAKHDEF
                     AAFTEEVDALGRTRRARGFDWAGFAVSGKGVFLEGVEVAFIVLTLGATSGGYAAPTLG
                     AGTALVLVVVAGAALRRPLTRVPENTLKYAVGAMLVTFGVYWTAEGLGVAWTGGAIAL
                     GYLLAATLALSWLSVRWLRRPEPAELAASR"
     gene            1937..2191
                     /locus_tag="VHH53_07680"
     CDS             1937..2191
                     /locus_tag="VHH53_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290059.1"
                     /translation="MLKTVMTAGRQAFGLLVDDGAVAVGILVAVGVAALLSFSGLFGS
                     GDATGWVLFTLVWLALAVSLRRARSRLSQVGGEPGNKPAT"
     gene            2210..2620
                     /locus_tag="VHH53_07685"
     CDS             2210..2620
                     /locus_tag="VHH53_07685"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX2290060.1"
                     /translation="MVTPREGLERLRAAAASGELHALCQRWHVNLLTVFGSAGRGEPD
                     PRDLDIAVLTEHGADFDLFRFITEVVDIVGLEQVDVAHLNAAGPLLRERALVGSNILY
                     ESEPGTWARISTAAVMERMDTEWLRRLSLELLAG"
     gene            2617..>2923
                     /locus_tag="VHH53_07690"
     CDS             2617..>2923
                     /locus_tag="VHH53_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX2290061.1"
                     /translation="MTPRRLDPPVILKRLREIQLLLAGLVEIGEVDAERLRRERSTRH
                     VVERVLSQIVELAGSINTHIVAALLGRSPESYADSFDEVARAGVLEWDLAAALRPSAG
                     "
BASE COUNT          442 a          938 c         1051 g          492 t
ORIGIN      
        1 agaacgccag gctcgcggcg gcgaaggtgc ctgactgact tgtcagccag ccagagaacc
       61 gcatctgcaa cgaatcccgg aacataccaa tgattcactg cagattcggc ggtcggccga
      121 acctggttga tctttccgga aaggtcggac gaggaatgtt cacggcgagg aattccatgc
      181 cgacaactgc ctgcaagcgg tagccatagc ttccgcgtgc ggtcggggag cgtaatccac
      241 tgcggcctgg gcggacaact agcgaggatc tgcgtcgggg gtatcgacgt cggcgggaac
      301 gccgagcagc tcgccgcgca gccggttgag ctcgctcagc gcggtggtgg cacgggcggg
      361 tccagtggcg gccagtgcga cagcggtggc ttcgagcagc agcagcgtga cggcatggct
      421 ggactgctcg ccaggcattc cacgcgggca gctgagcacg aggtcggcgc catcgacgtc
      481 ggccagcacg tcggtcacca gcaagacggg gatccgccgg cgtcgagcca gaccgatcag
      541 gaccgcggcg tgggtgtgca ggcgggtata ggccagcagc agcagcgcgt cgccgcggcg
      601 cagtcccatg gcttgatcgg ccatcgacac cccggtagca gtgatcgtgg tcgccggtcg
      661 gcctacccga cccaggagca cggcggcgta ctgcgccacg gcggccgagg gccccgtgcc
      721 actgagcacg atccgctcgg cggcggccaa cagtgccacc gcggccggga attgctcatc
      781 aaggctgttc cgagcggcgt tcagcgcggc ccggtgtacg tccaggacgt gttccagcac
      841 atccctcggc gccgtcgcgt gccgcagggt tgcgcccagc cgagcgctcg gcgtgggttc
      901 cggcggcggt gcgtcgtcaa gcagcgcccg cttgagatcc gtcagaccgc ggtagcccag
      961 agtctgggca gtgcgcacca cggtggcatc gctggtgccc agccgggcag cgatctccgc
     1021 ggcggagaag aacccgagct gggtggcgtg ctcggtgaag aaccgggcga ccctgcgctg
     1081 cgccggcgcg aggccgccca gcccggcagc gatccggtcg gcgacatggc gagtcatcta
     1141 atactcctct ggagctaggg cgtagcaaat gctaccttcg gttggtgctg gatctggtgg
     1201 cggtcgcatt gcccgctttt ctggcatccc tggtggagtt cgtcgaggct ttcacaatcg
     1261 tgcttgcggt aggcaccagt cgaaattggc gggctgcact ggccggtgca gcgacggcca
     1321 ccgtcgcact ggccgcgctg ctgatcgcgt tcgggcctgg gctgctcgag cgggtcgacg
     1381 agcacgtgct cttgctggtc atcgggatcc tgttgctact gttcggcggc cggtggctgt
     1441 gcaaggccat gctgcgttcg gcggggctga tcgccaagca cgacgagttc gccgccttca
     1501 ctgaggaggt cgacgcactc ggccggacac ggcgcgcccg cgggttcgac tgggccggat
     1561 tcgccgtcag cggcaaggga gtgttcctcg aaggtgtgga ggtggcgttc atcgtgctca
     1621 ctctcggcgc taccagcggc gggtacgccg ctcccacctt gggtgccggc accgccctgg
     1681 tgctggtcgt cgtggctggc gcggcgttgc gtcgaccgct gacccgggtc ccggagaaca
     1741 cactcaagta cgccgtcggc gcgatgctgg tcactttcgg ggtgtactgg accgcggagg
     1801 gcctcggcgt ggcctggacc ggcggcgcca tcgcgctggg ttacctgctc gccgccacgt
     1861 tggcgctgtc ctggctcagc gtgcggtggt tgcgccgccc ggagcccgct gagctggcgg
     1921 cgtctcggtg agcgcggtgc tcaagacggt catgaccgca gggcgccaag cgtttggcct
     1981 actagtcgac gacggcgcag tggccgtcgg catcttggtc gcggtcggcg tggccgcatt
     2041 gctcagcttc tctggactgt tcggctctgg cgacgcgacc ggctgggtgc tgttcacgct
     2101 cgtgtggctg gcactggcgg tgtcgctgcg ccgagcccgc tctcggttga gccaggttgg
     2161 cggcgagccg gggaacaaac cagcgacctg atgcaggaca atggcagccg tggtgacacc
     2221 gcgtgaggga ctggaaagac tgcgcgccgc ggcggcgtcc ggtgagctgc acgcgctgtg
     2281 ccagcggtgg cacgtcaacc tgctcacggt tttcggtagc gccggccggg gcgaaccgga
     2341 tccgcgagat ctcgacatcg cagtcctgac cgaacacggc gctgacttcg acctgttcag
     2401 gttcatcacc gaggtggtcg acatcgtagg cctggagcag gtggacgtgg cccacctcaa
     2461 cgccgctgga ccgctgctgc gagaacgtgc cctggtcggc tcgaacattc tctatgagag
     2521 cgaacccgga acgtgggcac gtatatccac ggctgctgtc atggagcgga tggacaccga
     2581 gtggctgcgc cggctgagcc tggaactact ggcaggatga cgccgcgcag gctggaccca
     2641 cccgtcatcc tgaagcggct gcgcgagatc cagctgctcc ttgccggcct cgtcgagatt
     2701 ggcgaagtgg acgccgagcg gctgcggcgt gaacgatcga cccggcacgt cgtcgagcgc
     2761 gtgctcagcc agatcgttga gctcgccgga tcgatcaaca cccacatcgt ggcagcgctg
     2821 cttggccgct caccagaaag ctacgctgac tcgttcgacg aggttgcgcg agccggcgtc
     2881 ctcgaatggg acctcgccgc cgcactgcgc ccgtccgctg gca
//
LOCUS       DATADN010000594         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_213499, whole genome shotgun sequence.
ACCESSION   DATADN010000594 DATADN010000000
VERSION     DATADN010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213499"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VHH53_07695"
     CDS             <1..1740
                     /locus_tag="VHH53_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEX2290062.1"
                     /translation="QAAQRAGAATGPDDLRDLAGEIEWAKASLVTAADYPAAVAAASR
                     DTPAPAAQIAAVYAGYEEIKNRSRVLDFDDLLLHTAAALEEHPGVAQEFRDRYRCFVV
                     DEYQDVTPLQQRVLDAWLGARDDLTVVGDPNQTIYSFAGAAPRFLLEFTRRFPEAVTV
                     QLRRDYRSTPQIVSLANRVISAAAGRPAGARLQLEGQQPAGPEPSFDEHDDEPTEAIT
                     VVEAIAELLGQGMAAAEIAVLYRVNAQSEAYEQALDARGIPFQIRGGERFFQRPEVRA
                     AIHALRAAATAGTVHKSAGDLGATAREVLAAHGLTTQPPGGGAARSRWESLRALAELA
                     TDLAAGTPGADLGQLVTELDARADAAHPPAMQGVTLASLHAAKGLEWDAVFLVGLIEG
                     TMPIQYADGDQAALEEERRLLYVGVTRARRWLALSWALSRSAGGRRSRHRSRFLHGLV
                     PQGSPAVRPTGSNAQPKAMLQTASGKTYCRICGIPVTGTIAVKLARCATCPSDVDPEL
                     LDRLRDWRTGCAKEQRVPAFVIFTDATLTAIAEQRPADAAGLTAIPGIGAAKLDRYGA
                     KVLALVRGGSVSG"
BASE COUNT          374 a          814 c          823 g          379 t
ORIGIN      
        1 caggccgcgc agcgagccgg cgctgcgacg ggtcctgacg acctgcgcga tctcgctggc
       61 gagatcgagt gggccaaagc cagcctggtc accgcagcgg actacccggc tgcggtggcc
      121 gcggcgagcc gagacacccc agcaccggcg gcgcagatcg cggccgtcta cgccggatat
      181 gaggagatca aaaaccgcag ccgggtactc gacttcgatg atctgttgct gcacaccgcg
      241 gccgcgctcg aggagcaccc gggagtcgcg caggaattcc gggaccgcta ccgctgcttc
      301 gttgtcgatg agtaccagga cgtcacaccg ctgcagcagc gcgtgctgga tgcctggctg
      361 ggtgcgcgtg acgacctcac cgtcgtcggt gacccgaacc agaccattta ctccttcgcc
      421 ggtgcggccc cccgattcct actggagttc accaggcgat tccccgaggc ggtgacagtg
      481 cagctgcgcc gcgattaccg ctccacgccg cagatcgtgt cactggcgaa tcgggtgatc
      541 tctgctgcgg ccggccgtcc ggcgggtgcc cggctgcagc tggagggcca gcagccagcc
      601 ggcccggagc cgtctttcga cgagcacgac gacgagccta cggaggccat caccgtggtg
      661 gaggcgatcg ccgagttgct gggtcaggga atggcggccg ccgagatcgc cgtgctgtac
      721 cgggtcaacg cccagtccga ggcctacgag caggcattgg acgcccgcgg gatcccgttt
      781 cagatccgcg gcggagagcg attcttccag cgtccggagg ttcgggctgc gatccacgcg
      841 ctgcgggcgg cggccaccgc aggcaccgtg cataagtcgg cgggggattt gggtgccacg
      901 gcccgcgagg tactcgccgc ccacgggctc accacccaac cccctggggg cggtgccgcg
      961 cggtcacgct gggagtcgct gcgcgcgctg gctgagctgg ccacagacct cgcggccggc
     1021 acgccaggcg ccgacctggg ccagctggtg accgagctcg acgctcgcgc cgacgccgcg
     1081 cacccaccgg cgatgcaggg cgtgacgctg gcctccttgc atgccgcgaa gggcctggag
     1141 tgggacgcgg tgttcctcgt cgggctcatc gagggaacga tgccgatcca gtacgccgat
     1201 ggtgaccagg ccgcgttgga ggaggagcgc cggctgctct acgtcggcgt gacccgggcc
     1261 cgccgttggc tagcgctgtc ttgggcgcta tcgcgcagtg caggcggccg acgctcgcgg
     1321 catcgcagcc gctttcttca cggcctggtg ccccagggtt ccccggctgt gcggccgacc
     1381 ggctccaacg cgcaaccgaa ggcaatgttg cagacggcgt ctggcaagac ttattgccgg
     1441 atctgtggca tcccggtcac tgggacgatc gcggtgaagc tggcccgatg tgccacgtgc
     1501 ccatctgatg tcgacccgga actgctggat cgattacggg actggcgcac tggttgcgcg
     1561 aaggagcagc gggttcccgc cttcgtgatc ttcaccgatg ccacgctgac cgccatcgcc
     1621 gagcaacggc cagccgacgc cgccggcctg acagccatcc cggggatcgg agcagccaag
     1681 ctggaccgct atggggccaa agtactcgcc ctcgtccgcg gcggttcggt cagtggttga
     1741 caaagaaagt acgaaattcg gttgctggtg tatcagcagg gacctaacct tgctcataat
     1801 ctttggaggc ggccgttgca cgctgctgac caggatagat cgcccagcta ctgaacgtgg
     1861 gccgagcggg aagggaggcg ccgtgatgga gatctgcaca gccaccggcg cccgttgcta
     1921 cccgcagcgc gggcacctcg ctatgccagg agaggtctgg tccaagcgct tgccgaccgc
     1981 gccgtctggc gcgcctcggc tgcgtgccgg gcgttccctt caccgcagcg tcatcaaccc
     2041 gaccggcact gacctgtcgg tgaccaaccg catgcgtacg tctgcatcgg agccttgatc
     2101 ctcgagccag caccaaccgg ctcatgaagg ccgcggaccc cgcagcggga tccgcggcct
     2161 ttgtgcgttg ctaggtcctc atcccgcggc gggcacggta ccggcgattt gcactggcac
     2221 cacaccgagg aggagagaca agtgttgacc gcgaccgtcc cgctggatgg cggggcgttg
     2281 cccgacctgc tgggtggggc agagctggcc cacctgctcg ataccgcgcc ttttgggtcg
     2341 ggcaccctgc cttgccgctc acacaacccg gacctgtggt tcgccgaggc
//
LOCUS       DATADN010000595         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1135569, whole genome shotgun sequence.
ACCESSION   DATADN010000595 DATADN010000000
VERSION     DATADN010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135569"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1797)
                     /locus_tag="VHH53_07700"
     CDS             complement(<1..>1797)
                     /locus_tag="VHH53_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX2290063.1"
                     /translation="ALPRSVELIIALWAVLKAGAAYLPVDPDFPSERIRFVLDDAGPV
                     VVFDDPLTVRDTDGYPDINPTDADRIHPLEPVNPAYVIYTSGSTGRPKGVVVPHCGIV
                     NRLLWMQAEYGLREDDRVLQKTPSSFDVSVWEFFWPLIVGATLVVAKPEGHKDPAYLA
                     GLIRSEAVTTIHFVPSMLRAFLQDQAAASCTGLRRVICSGEALPADLARDFHSVLDVG
                     LHNLYGPTEASVDVTYFQCVPQQHAVSVPIGRPVWNTGMYVLDRNLRPVPTGVPGELY
                     IAGVQLARGYLNRPGLTAERFVACPFGAPGARMYHTGDLASWSPDGNLMYLGRTDHQV
                     KIRGFRIELGEIETVLLRRPEVAAAVVVARQEHSDHKRLVAYLIPAVDDVVNSAELRE
                     FLRQVLPDYMVPSAFVTLDAFPLSPNGKLDRRALPAPDWSASTRAGYVPPHTDVERVL
                     VQLWAEVLDVEQVGVEDNFLELGGDSILSIQVVSRICATFGVYLSSRAVFDAPTVAQL
                     ADLLPTAPRADHVERITRVPRERTLPLSLAQQRLWLLAGMTSGSTEYNNGFGLRLSGA
                     LDLDALRAVLDALVRRHESLRTTFDTVDGHGVQ"
BASE COUNT          382 a          589 c          533 g          293 t
ORIGIN      
        1 ctggactccg tgcccatcta cggtgtcgaa ggttgtccgc agcgactcat gccggcgcac
       61 caaagcgtcc aacaccgccc gcagcgcgtc gaggtccaac gcaccggaca gtcgtagccc
      121 gaatccgttg ttgtattcag tgctgccgga agtcataccg gccagcaacc acaaccgttg
      181 ctgggcgaga gaaagcggta gtgttcgctc ccgcggtacg cgggtaatcc gctcgacgtg
      241 atcagcgcgg ggcgctgttg gcagcaggtc cgcgagttgc gcaacggtcg gggcgtcgaa
      301 caccgcgcgg gaggacaggt acaccccgaa ggtcgcgcag atccgggaca ccacctgaat
      361 gctcaggatc gagtcgccgc cgagctccaa gaagttgtcc tcgacaccaa cctgctccac
      421 atcgagcact tcggcccata gctgaacaag caccctctcg acgtcggtgt gaggcggtac
      481 atatccggcg cgcgtactcg cgctccagtc cggagcgggt aaggcacgcc gatcgagctt
      541 gccgttggga gaaagtggga atgcatcaag tgtcacaaac gctgagggaa ccatgtaatc
      601 gggcagcacc tgacgcagga attcgcgtag ctccgcggag ttcaccacgt catcgaccgc
      661 aggaatgaga taggccacca accgcttatg gtcggaatgc tcctgccggg caaccacgac
      721 cgccgcagca acctcgggtc gccgcagcag caccgtctcg atctcaccca gctcaatccg
      781 gaacccacga atctttacct ggtggtcggt tcgaccgaga tacatgaggt tgccgtccgg
      841 gctccacgac gcgaggtccc cggtgtgata catccgggca ccgggcgccc cgaatgggca
      901 ggccacaaac cgttccgcgg tcagaccggg ccgattgaga tacccacggg ccagttgcac
      961 tccggcgatg tacagctcgc cgggcacccc cgtcggcact ggacgcagat tgcggtcaag
     1021 cacatacatc ccggtgttcc acaccggacg cccaatcgga accgacacgg cgtgctgctg
     1081 ggggacacac tggaagtagg tcacgtccac cgatgcctca gtggggccat acaggttgtg
     1141 caggcccaca tcgagcacgg agtggaagtc tcgggcgagg tctgcaggca atgcctcacc
     1201 gctacagatg acccgccgca acccggtaca gctcgccgct gcttgatcct gcaaaaatgc
     1261 acgtaacatc gacggcacga agtggatcgt ggttaccgct tcagatcgga tcaatccggc
     1321 caagtatgct gggtctttgt ggccctcggg tttggccacg accagcgtgg cgcccactat
     1381 cagcggccag aagaactccc acaccgatac gtcgaagctc gacggcgtct tttgcagcac
     1441 ccgatcgtct tcgcgcagac cgtactcagc ttgcatccac agcaaccggt tcacaatccc
     1501 gcaatgcgga acgacaacac cctttggccg gccagtggaa cctgatgtgt aaatcacata
     1561 cgccgggttg accggctcca gcggatgaat acggtcagca tcggtcgggt taatgtctgg
     1621 gtaaccgtcg gtgtcccgaa cggtcagcgg gtcgtcaaac accaccacgg gaccggcgtc
     1681 atcgagcacg aacctgatcc gctcggacgg gaaatccgga tccaccggca ggtaagcagc
     1741 cccggccttc agcaccgccc acagcgcaat gatcagctcc actgaccgcg gcaaggc
//
LOCUS       DATADN010000596         1701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1266510, whole genome shotgun sequence.
ACCESSION   DATADN010000596 DATADN010000000
VERSION     DATADN010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266510"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VHH53_07705"
     CDS             <1..676
                     /locus_tag="VHH53_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX2290064.1"
                     /translation="PEQLGAFFTWQIAPPLAFVPAERHGDLFCAMIVCWSGPINDGEQ
                     AIKPFRDAAEVKAEHVGPMPYPALNSAFDGLVPRGLQHYWKADYVAELTDDAIQAHIE
                     HGKKTPVVNSTMHLYSINGAAHRVGPDETAFGHRDAKYAAVIAGMWPDPADNDANITW
                     VRDYYAAIHPHSGTEGGYINFMSGDDAGRAPENYGPNYERLRGVKAAYDPGNLFHLNQ
                     NIEPAM"
     gene            807..1493
                     /locus_tag="VHH53_07710"
     CDS             807..1493
                     /locus_tag="VHH53_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290065.1"
                     /translation="MSPSSTPVIGNSMAGLRSARSRRLLIDSAADVAYAARALAAGGV
                     TATAFGNFYVIVTRPAVKTVRRVNVVKGRPIHQVGSITTEASRIPAMFDWSQLPERRT
                     AERVQALMETLYGFGPFGFRGPAAAHVPDHLTQLDGGVRTTQVIAPGSRCPSNAFFAR
                     AVAEVGAELLYITSANRSRHLTGAVEEPAHWQAAGIAADFAHVPDLVIIEHRDEGAAR
                     EAYPGSLLRR"
     gene            complement(1496..>1701)
                     /locus_tag="VHH53_07715"
     CDS             complement(1496..>1701)
                     /locus_tag="VHH53_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290066.1"
                     /translation="SAGRSCAHRRLGIVPDLQPLLDVLPEHHVEAEWFGNVAYRADRQ
                     RTVPVDGEHRPSTGRAAEGMERQ"
BASE COUNT          288 a          590 c          547 g          276 t
ORIGIN      
        1 gcccgaacag ctcggggcgt ttttcacctg gcagatcgcc ccgccgctgg cgttcgtacc
       61 cgccgagcgc catggtgatc tattctgcgc catgatcgtg tgttggagcg ggccgatcaa
      121 tgacggcgag caggccatca agccgttcag ggacgcggcc gaggtgaagg ccgagcatgt
      181 cggaccgatg ccctatcccg cgctgaattc ggcattcgac ggtcttgtgc cgcgcggcct
      241 gcagcactac tggaaggccg actacgttgc cgaactcacc gatgacgcca tccaggcgca
      301 catcgagcac ggcaagaaga caccggttgt caattccacc atgcacctgt attccatcaa
      361 cggcgccgcc caccgcgtcg ggcccgacga gaccgcgttc ggtcaccgcg acgccaagta
      421 cgcggcggtc atcgccggca tgtggcccga tccggcagac aacgacgcga atatcacgtg
      481 ggtacgggac tactacgcgg ctatccatcc tcactcaggt accgaagggg gctacatcaa
      541 cttcatgtcc ggggacgacg ccgggcgggc accggagaac tatggcccca actacgagcg
      601 actgcgcgga gtgaaagccg cctacgaccc cgggaacctt ttccacctca accagaacat
      661 cgaaccggca atgtgagaga gctccgcgcg tatcagggag tgcagctgcc agctctcaac
      721 cgttggaggc gatgacctgc cggcgtgacc cgctggcagg tgtgcttcag cgagagggtc
      781 gacccatagc ccggaagggt cgctacatga gccccagctc gacacctgtg atcggcaaca
      841 gcatggcggg cctgcgctcg gctcgctccc gacgcttgct catcgattcc gcggccgacg
      901 tcgcctacgc cgctcgggcg ctcgccgcgg gcggcgtcac ggctaccgca ttcggcaact
      961 tctatgtgat cgtcacccga cccgccgtca agacggtccg ccgggtcaac gtggtcaagg
     1021 gccgtcccat ccatcaggtc ggcagcatca ccaccgaggc gagtcgcatc ccagccatgt
     1081 tcgactggtc gcagctgccc gagcggcgta ctgctgagcg cgtgcaggca ctcatggaga
     1141 ccctgtacgg gttcggcccg ttcggcttcc ggggtccggc cgccgcgcac gtgcctgatc
     1201 acctcaccca acttgacggg ggcgtgcgca ccacccaggt gatcgcaccc ggctcccgct
     1261 gcccgtcaaa cgctttcttc gcacgcgccg tggcggaagt gggtgctgag ctgctgtaca
     1321 tcacctcggc gaaccggtcg cggcacctca ccggcgcggt ggaggagccc gcgcactggc
     1381 aggcggccgg aatcgccgcc gacttcgcgc acgtgcccga cctggtgatc atcgagcatc
     1441 gcgatgaggg ggccgcccgg gaggcgtatc cgggttcgct cctacgtcgg tgaccctact
     1501 gtcgttccat gccctcggcg gctcgcccgg tggaggggcg gtgctcaccg tcgaccggca
     1561 cggttcgttg ccgatcagcg cggtacgcca cgttgccgaa ccattcggct tcgacgtggt
     1621 gctcgggcag cacgtccagc aacggctgca gatccgggac tatcccgagg cggcggtggg
     1681 cgcatgagcg cccggcggat c
//
LOCUS       DATADN010000597         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_382838, whole genome shotgun sequence.
ACCESSION   DATADN010000597 DATADN010000000
VERSION     DATADN010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382838"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VHH53_07720"
     CDS             complement(<1..355)
                     /locus_tag="VHH53_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2290067.1"
                     /translation="MIIMNADELIAGATNLRPILERNADCADRDRRLPEETVNAMHEA
                     GVFRILTPRCFGGCEADLGTLVEVVGQISQGCCSAGWLTLLMQSCTWLAALYPEQAQE
                     EVFASGPDARAAGVLA"
     gene            complement(336..>1777)
                     /locus_tag="VHH53_07725"
     CDS             complement(336..>1777)
                     /locus_tag="VHH53_07725"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX2290068.1"
                     /translation="VVLLPPSSGSATARTAGFDFDRGNAILELVIPTVTPKLLTTIAP
                     SDATIFLRVATLLNNGWFDAIAPYHPTAVGVSSRLGRRPAHERTTHRQRNISLLHASY
                     HILNSVLPQFPQQWRTMLTTVGLNPDDTSTDLTTPVGIGNRAGHAVVAAREHDGMNQL
                     GDAGGRRYHRQPYADTTGYRSVNTPGELRDPSRWQPNLVTNGGGIFRAQEFVTPQYGR
                     TRPYAYRHPAQFRVPPPVNSDAQHNPRGYRRQADEVLAVSAGLDDRRKLTAEHFDSKE
                     SLVTSVIHIAEARHFGLDEFVHLEFLTNLAAFDVGIAVWHYKARYDAVRPFSAIRHLY
                     GDQRITAWGGPGQGRVTDITGAEWRSYLTTADHPEYPSGSASFCAAHATAARLFTGTD
                     ELGQTVSIARGASVVEPGVTPARDTTLTWRTWTQWAAECGRSRLWGGVHFHDSIPAGQ
                     KLGAQIGERAYQFVIAHLTGQRGDDHHER"
BASE COUNT          253 a          540 c          689 g          295 t
ORIGIN      
        1 gtgccagtac cccggcggct cgtgcgtctg ggcctgaggc gaacacctct tcctgggcct
       61 gctcggggta caacgcggcg agccaggtgc aggactgcat caacaacgtc agccatccag
      121 ccgagcagca cccctgcgag atctgcccga cgacttccac cagcgtgcca aggtctgcct
      181 cacagccgcc gaaacatcgg ggcgtcagga tgcggaagac gcccgcctca tgcatcgcat
      241 tgaccgtctc ctcgggcagt cggcggtcac ggtcggcgca gtcggcgttg cgctccagga
      301 ttggccgcag gttggtggca cccgcgatca actcgtcagc gttcatgatg atcatctccg
      361 cgctgcccgg ttaggtgggc gatgacgaac tggtaggcgc gttcgccgat ctgggcgccc
      421 agcttctgtc cggcggggat ggagtcatgg aagtggacgc cgccccacaa gcggctccgc
      481 ccgcattcgg cggcccactg cgtccaggtg cgccaggtca gggtggtgtc ccgggcgggg
      541 gtgacgccgg gctcgacaac ggaggcgccc cgggcgatcg agacggtctg gcccagctcg
      601 tcggtgccgg tgaacaggcg ggccgccgta gcgtgtgcgg cgcagaaact ggccgacccc
      661 gaggggtact ccgggtgatc agcggtggtg aggtagctgc gccactcggc gccggtgatg
      721 tcggtaaccc ggccctgccc tggcccgccc cacgcggtga tccgctggtc gccatacagg
      781 tggcggatcg cgctgaacgg ccgtaccgcg tcgtagcgcg ccttgtagtg ccagacggcg
      841 atgccgacat cgaacgcggc caggttggtc aggaactcca ggtgcacgaa ctcgtccagg
      901 ccgaagtggc gggcctcggc gatgtggatg acggaggtga cgaggctttc cttgctgtcg
      961 aagtgctcgg cggtcagctt gcggcggtcg tcgagcccgg cggagacggc gagcacctca
     1021 tcggcctgtc ggcggtaccc gcgggggttg tgctgggcgt cgctgttgac cggtggtggc
     1081 acgcggaatt gggccgggtg gcggtaggcg taggggcggg tgcggccgta ctgcggggtg
     1141 acgaactcct gggcgcggaa gatgcctccg ccattggtga ccaggttggg ttgccagcgg
     1201 gacgggtcac gcagctcgcc gggggtgttg acggatcggt agccggtggt gtcggcgtag
     1261 ggctgccggt ggtagcggcg gccgccggcg tcaccgagct ggttcattcc gtcgtgctcg
     1321 cgggcggcga ccacggcgtg gccggcccgg ttcccgatgc cgaccggtgt ggtgaggtcg
     1381 gtgctggtgt cgtcggggtt gagcccgacc gtggtcagca tggtccgcca ctgctgcggg
     1441 aactgcggaa ggacgctgtt gaggatgtgg taggaggcgt gcagcagcga gatgttgcgc
     1501 tggcggtgtg tggtgcgctc gtgggccggg cgccggccca gtcgcgatga gacccccacc
     1561 gcggtgggat ggtaaggggc gatggcgtcg aaccacccgt tgttgagcaa tgtggcgacc
     1621 cgcaggaaga tcgtggcatc gctcggcgcg atggtcgtca gtaacttcgg cgtgacggtc
     1681 gggatgacca actcgaggat ggcgttgccc cggtcgaaat cgaacccggc ggtgcgggcg
     1741 gtcgcgctac ctgacgaggg cggcagcagc accacca
//
LOCUS       DATADN010000598         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1266949, whole genome shotgun sequence.
ACCESSION   DATADN010000598 DATADN010000000
VERSION     DATADN010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266949"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1271)
                     /locus_tag="VHH53_07730"
     CDS             complement(54..1271)
                     /locus_tag="VHH53_07730"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX2290069.1"
                     /translation="MTPGKGVTDASALLLSCAAGARLRTPGPEAGAARRSRISPDTVL
                     VHADLHNHTLLSDGAGDPEAAFPSMRDAGLDVAALTDHAIRGAAVEGTGLGGTPWIGL
                     DRAGWRRTGKLADTYDRPGEFTAIRGFEWSHPHLGHVNAWFTDDFTDVATHGAMAELY
                     AWLTRFGRRNGATPPAGLAGFNHPCREKGAFAHFRYTASAAEQVVSLEMFNRCDDYLF
                     QGVAAGGTSPLVACLDAGWRTGLLGVSDEHGSDWGLPEGRGRAGLWVAEHSRAGVAEA
                     LRARRFFATRVPGLRVDATANEVRMGSVLRHRHGCVAFAFDVDGGPSWLGRPLQAQVL
                     RPGTSVPAVADVIEMRCGDVAHFTVALDIEEGHWVVLRLADPAVGNGTQGPPGHPANN
                     HGLAYTSPWFLEP"
     gene            complement(1268..2176)
                     /gene="pdxS"
                     /locus_tag="VHH53_07735"
     CDS             complement(1268..2176)
                     /gene="pdxS"
                     /locus_tag="VHH53_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005445300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2290070.1"
                     /translation="MLAENINTERQIGTALVKRGMAEMLKGGVIMDVVTPEQAKIAED
                     AGAVAVMALERVPADIRAQGGVARMSDPDMITGIIEAVSIPVMAKARIGHFVEAQVLQ
                     AIGVDYVDESEVLTPADEAHHIDKFAFTVPFVCGATNLGEALRRISEGAAMIRSKGEA
                     GTGNVVEATRHMRSIRASIRALAAMPPEELFAAAKDLAAPVELVREVAEAGSLPVVLF
                     TAGGIATPADAAMMMQLGAEGVFVGSGIFKSGDPAKRAEAIVKATTFHDDPEMVAKVS
                     RGLGEAMVGINIGELAEGQRLAQRGW"
     gene            2362..>3503
                     /locus_tag="VHH53_07740"
     CDS             2362..>3503
                     /locus_tag="VHH53_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2290071.1"
                     /translation="MGTKGNGERPDPAVAAQDPSRIRNVALVGPSGAGKTTLAEALLA
                     ATGVISRAGTVTDGTTVCDHDPAAVRQQRSVTLAIAPLQHAGIKINLVDTPGYADFVG
                     ELRAGLRAADAVLFVVSAGDGVDPATVALWQECADAGMPRAVVVSRLDHPRADLDGAM
                     VSCRAAFGDGVLPLYLPVRDGAGAVTGLVGLLSQRLFDYRDGYPPVKGETNPVGSVVD
                     ADEFAVARNELIEGIIAESEDESLMDSYLGGEDLDVEALIADLETAVTRGSFHPVIPA
                     CASTGVGLAELLEVLTDAFPAPGKHTPPPVTRSDGSAHSVVDVDPTAALVAEVVRTTV
                     DPYQGRVSLVRVFSGTLHPDRPVHVSGRGLADRGHADHDVDERVAHV"
BASE COUNT          567 a         1219 c         1181 g          536 t
ORIGIN      
        1 tgaaagtctt cgaagcagat cgcggctccg gacccggtac gaacgtgacg cggtcagggc
       61 tcgaggaacc acgggctggt gtaggcaagc ccgtggttat tcgcagggtg gccgggcgga
      121 ccctgcgtgc cgttaccaac cgcggggtcg gccagccgca gcaccaccca atgcccctcc
      181 tcgatatcca acgccacggt gaagtgggcg acatcaccac agcgcatctc gatgacatcg
      241 gccactgcag gcaccgacgt gcctggacgc agcacttggg cctgcagcgg gcggccgagc
      301 caggagggcc cgccatcgac gtcgaatgcg aatgccacgc agccatggcg gtgtcgcagc
      361 actgagccca tgcgaacctc attcgcggtg gcgtccaccc gcagtcccgg gactcgggta
      421 gcgaaaaacc gccgggcccg tagcgcctcc gcgaccccgg ctcggctgtg ctcggcgacc
      481 cacagcccgg cccgtcccct accctccggc aatccccagt cgctgccgtg ctcgtcgctg
      541 acgccaagca gcccagtccg ccaaccagcg tccaggcagg ccaccaaagg agaggtaccg
      601 cccgccgcaa cgccctggaa caggtagtca tcgcagcggt tgaacatctc caggctgact
      661 acctgctcgg cggctgaggc cgtatagcga aagtgtgcga aggcgccctt ctcccggcag
      721 ggatggttga agccggcaag cccagccggg ggggtggcgc cgttgcgtcg gccgaaccgg
      781 gtcagccagg catacagctc ggccatcgcc ccgtgcgtcg ccacgtcagt gaagtcatcg
      841 gtgaaccacg cattgacatg ccccagatgc ggatgtgacc actcgaagcc gcggatcgcg
      901 gtgaactcgc cgggccggtc gtaggtatcg gcgagctttc cggtgcgccg ccaaccggcc
      961 cggtccagcc cgatccacgg cgtgccgcct agcccagtgc cctccaccgc ggccccgcgg
     1021 atcgcgtgat cggtgagtgc tgcgacgtcc aggccggcat cgcgcatcga gggaaacgcc
     1081 gcttctggat cgcctgcacc gtcggaaagc aacgtgtggt tgtgcaggtc agcgtggacc
     1141 agcacggtgt cggggctgat ccgggaacga cgggcggccc cggcctcggg ccccggagtg
     1201 cgcagccgcg cacctgcggc gcaagacagc agcagcgcgc tggcgtcggt gacacctttg
     1261 ccgggggtca ccagccgcgc tgggccagcc gctgcccttc ggccagctcg ccgatgttga
     1321 tccctaccat cgcctcgccc agtccccggg acaccttcgc caccatctcc ggatcatcgt
     1381 ggaacgtggt ggccttgacg atcgcctccg cacgcttggc agggtcaccg gacttgaaga
     1441 tgcccgagcc gacgaacacg ccctcggcgc ccagctgcat catcatcgcc gcgtcggcgg
     1501 gcgtggcgat accaccagcg gtgaacagca ccactggcag cgagccggcc tcggcgacct
     1561 cgcggaccaa ctcgaccgga gccgccaggt ccttggctgc cgcgaacagc tcctcgggtg
     1621 gcattgctgc cagggcgcgg atgctcgcgc ggatcgagcg catgtgccgg gtcgcctcga
     1681 cgacattgcc ggtcccggct tcacccttgg accggatcat ggccgcgccc tcggagatcc
     1741 ggcgcaacgc ctcacccaga ttggtagcgc cgcagacgaa cggcacggtg aaagcaaact
     1801 tgtcgatgtg gtgggcctca tcagccgggg tcagcacctc ggactcgtcg acatagtcca
     1861 cgccgatcgc ctgcagcacc tgggcctcga cgaaatgccc gatgcgggcc ttggccatga
     1921 ccgggataga caccgcctcg atgatcccag tgatcatgtc cgggtcactc atccgggcca
     1981 caccgccctg cgcgcggatg tcggcaggga cccgctccag cgccatgaca gccaccgcgc
     2041 cggcgtcttc ggcgatcttg gcctgctccg gcgtgaccac gtccatgatc acaccgccct
     2101 tgagcatctc ggccatcccg cgcttgacca gtgcggtacc gatctgacgc tcggtgttga
     2161 tgttctcggc cagcacggcg gttcgctcct cagggcagta gcacgagaca agcactgggc
     2221 ctcaagtact tgagacccgg tactcgatcg tacgtcgggg ccgctgacaa cgtcgcgggt
     2281 tgtaggctgc tgggggttgc ctcgacccgg cacaccggtg tgagatcgac cacacggtcc
     2341 gcatagccga gggtgggtgc catgggcacg aaaggtaacg gtgagcgtcc tgatcctgcg
     2401 gtagcggcgc aggacccgtc tcggatccgc aatgtggccc tggtagggcc atccggggcg
     2461 gggaagacga cgttggcgga ggcactgctc gccgccaccg gtgtgatcag ccgcgcgggc
     2521 acggtcaccg acggcaccac ggtgtgtgac cacgacccgg ccgcggtacg ccagcaacgg
     2581 tcagtcacgc tggccatcgc gccactgcag cacgccggca tcaagatcaa tcttgtggac
     2641 acccccggtt acgccgactt cgtgggcgag ttgcgggctg ggctacgcgc agccgacgca
     2701 gtgctgttcg tggtctctgc tggagatggc gtcgatcccg cgacggtcgc gctctggcag
     2761 gagtgcgcgg acgccggaat gcctcgagcg gtggtggtaa gccggctgga ccacccacgg
     2821 gcggatctgg acggcgcgat ggtctcctgc cgggctgcct tcggcgacgg cgtgctgccg
     2881 ctgtacctgc cggtccgcga cggagccggt gcggtgaccg gtctggtggg cctgctgtcg
     2941 cagcgactgt tcgactatcg cgacggatac ccgccggtga aaggggaaac caacccggtg
     3001 ggcagcgtcg tggacgccga cgagttcgcc gtggcccgca atgaactcat tgagggaatc
     3061 atcgccgaga gcgaggacga gtccctcatg gacagctacc tcggcggcga ggatctcgac
     3121 gtcgaggcgc tgatcgcgga cttggagacc gcggtcacgc gcggttcttt tcacccggtg
     3181 atcccggcat gcgccagcac cggggtgggg ctggctgagc tcctcgaggt gctcactgac
     3241 gccttccctg ctccgggcaa gcacacccct cccccagtga ctcgctccga tgggtcggcg
     3301 cactccgtgg tggacgtgga ccccaccgcg gcgctggtgg cagaggtggt acgcaccacc
     3361 gtcgacccct accaggggcg ggtatcgctg gtgcgggtgt tctccggaac gttgcacccc
     3421 gaccgcccgg tgcacgtctc cgggcgcggg ctggccgacc gcgggcacgc cgaccacgac
     3481 gtcgatgagc gggtggcaca cgt
//
LOCUS       DATADN010000599         2695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_529671, whole genome shotgun sequence.
ACCESSION   DATADN010000599 DATADN010000000
VERSION     DATADN010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529671"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..968)
                     /gene="mutM"
                     /locus_tag="VHH53_07745"
     CDS             complement(114..968)
                     /gene="mutM"
                     /locus_tag="VHH53_07745"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX2290072.1"
                     /translation="MPELPEVEVVRLGLAQHVAGRTVAAVEVRHPRSIRRHIAGELDF
                     AARLAGRHVHAARRRGKYLWLDLGGEEALLAHLGMSGQMRIRPPGAVDETHLRVRLAF
                     ADGGSELRFIDQRTFGGLSVEELLDGLPAPVAHIARDPMDPCFDLDAVVTEIRRRRTG
                     VKRALLDQTLVSGIGNIYADEALWRARLHWAQPTATLTRLQGRAVLTAATEVMMEALA
                     AGGTSFDALYVNINGASGYFDRSLAVYGQDGRPCPRCAIPIRRESFMNRSSYTCPRCQ
                     PRPRHPHS"
     gene            complement(973..1671)
                     /gene="rnc"
                     /locus_tag="VHH53_07750"
     CDS             complement(973..1671)
                     /gene="rnc"
                     /locus_tag="VHH53_07750"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX2290073.1"
                     /translation="MLSALGVEIDAERLTLALTHRSYAYENGGLEPNERLEFLGDAVL
                     GVVVTDHLFRTHPDLPEGQLAKLRASVVNMHALAGVARGLGVNGLGEYILLGRGEELT
                     GGRHKSSILADTVEALIGAVYLQHGMDAARQVVHRLFDPLLVMAPLLGAGLDWKTSLQ
                     ELTASNEMGVPEYRVVEEGPDHLKKFTATAVIAGVEQGTGQGRTKKEAEQRAAEVAWR
                     SLSELASGSAPPAG"
     gene            complement(1738..1920)
                     /gene="rpmF"
                     /locus_tag="VHH53_07755"
     CDS             complement(1738..1920)
                     /gene="rpmF"
                     /locus_tag="VHH53_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX2290074.1"
                     /translation="MAVPKRRMSRSNTRSRRAQWKATAPTLAACSNRACRQPKPPHLA
                     CPHCGKYDGRQVVRPA"
     gene            complement(1960..2532)
                     /locus_tag="VHH53_07760"
     CDS             complement(1960..2532)
                     /locus_tag="VHH53_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX2290075.1"
                     /translation="MPRHAASARPDPSSPWVIDTRKLGRRPGAMRSYHRDVPAPAGLE
                     LYVIGIPPGDQLALDVRLESVMEGVLASGAVAAPLAGQCARCLDPVVDRLEVELTELF
                     AYPDTATDESTDPDEVSRVVDDLVDLGPVVRDAVLLALPPAPLCRDDCPGLCPECGEK
                     RAELGTDHRHETIDPRWAVLKQRFGGDSQR"
BASE COUNT          445 a          932 c          896 g          422 t
ORIGIN      
        1 aggtcgtcgc cggactgact gcccttccgg cgcataccgt tgagcttgac tggaagtcgt
       61 gaacaacgtt tgggagctga ctatcgtgtt ggagccgcca atttcggccc ccctcacgag
      121 tgggggtggc gggggcgggg ctggcagcgc gggcaggtgt aggaggagcg gttcatgaac
      181 gactcccgcc ggatcgggat agcgcaccgc gggcagggcc ggccgtcctg gccgtagacc
      241 gccagcgagc ggtcgaaata gccggaggca ccgttgatat tgacgtagag cgcgtcgaag
      301 gatgtgccac cggcggccag ggcttccatc atcacctcgg tggcggcggt cagcactgcg
      361 cgaccctgca ggcgggtcag cgtcgctgtg ggctgagccc agtgcagccg ggcacgccac
      421 agcgcctcgt cggcatagat gttgccgatt ccggacacca gtgtctgatc gagcagtgcg
      481 cgtttgacac cggtgcgccg ccgccggatc tcggtgacta ccgcatcaag gtcgaagcac
      541 gggtccatcg gatcccgggc gatatgcgcc accggtgctg gcaggccgtc gagcagctcc
      601 tccactgaca ggccaccgaa ggtgcgttgg tctatgaaac gtagctcgga cccgccgtcg
      661 gcgaaagcca gccgcacccg caggtgtgtc tcgtcgacag ccccgggggg ccggatacgc
      721 atctgcccgc tcatgcccag atgggccagc aacgcctcct cgccacccag gtccagccag
      781 aggtacttgc cgcggcgccg ggccgcgtgc acgtgacggc cggccagccg agcggcgaaa
      841 tccagctcac cggcaatgtg gcggcgtatc gagcggggat ggcgcacttc gaccgctgcc
      901 acggtacgtc cggcgacgtg ctgagcgagg ccgagccgga cgacctccac ctcgggcaac
      961 tccggcatga cactatcccg ctggcggggc gctcccgctg gccagctcgg acagcgagcg
     1021 ccaggccacc tcagctgcac gctgctcggc ttctttcttg gtgcggccct gccccgttcc
     1081 ctgctccacg ccggctatca cggcggttgc ggtgaacttc ttgaggtggt cgggcccctc
     1141 ctcgaccact cggtactcag gtacccccat ctcgttactg gccgtgagct cttgaaggct
     1201 ggtcttccag tccaggcctg cgcccagtaa cggagccatg accagcagcg ggtcgaacag
     1261 ccggtgcacc acctggcggg cggcgtccat gccgtgctgc aggtacaccg cgccgatcag
     1321 cgcctcgacg gtatcggcca ggatgctgga cttgtgccgg cccccggtga gttcttcccc
     1381 ccgccccagc aggatgtact cacccaagcc attgacacca agcccgcggg caacgccagc
     1441 caacgcgtgc atgttgacca cgctggcacg cagcttggcg agctgaccct cgggtagatc
     1501 tggatgagtg cgaaacaggt gatcggtgac gaccaccccg agcacggcgt cgccgaggaa
     1561 ctccaggcgc tcgttgggtt cgaggccacc gttctcatag gcgtaggagc ggtgggtaag
     1621 cgcaagagtc agccgctcgg cgtcgatctc gacgccgagc gcgctaagca gcggagccag
     1681 atccgatcta tggccccgcg aggaccgagc ccccacggcg acgtcgtgtc gtcaggatca
     1741 ggccggccgg acgacctggc ggccgtcgta cttgccgcaa tgtggacagg ccaggtgcgg
     1801 aggcttcggc tgccggcacg cccggttcga acacgccgcg agagtcggcg cggtggcctt
     1861 ccactgggcc cgccgcgagc gggtgttcga ccgcgacatc ctgcgcttcg ggacggccac
     1921 gttagtcgtt ctcctcttcg ctaccgctga actcgattat taccgctggc tatcgccacc
     1981 gaaccgctgc ttgagtacgg cccagcgcgg atctatcgtc tcatgccgat gatccgtacc
     2041 gagttcggcc cgcttttcgc cgcactccgg acacaggcca gggcagtcgt cgcgacacag
     2101 cggggccggt ggcagcgcca gcagcaccgc gtcgcggacc accggtccga ggtcgaccag
     2161 gtcatcgaca acccggctga cctcgtcagg atcggtgctt tcgtcagtcg cggtatccgg
     2221 ataggcgaac agctcggtca gctcgacctc cagccgatcc accaccggat ccaggcatcg
     2281 ggcacactgc ccggccagcg gcgcggccac cgcaccagac gccagcacac cttccatgac
     2341 ggactccagc cgcacgtcca gcgccagctg gtctcccgga gggatcccga tcacgtaaag
     2401 ctccaacccc gccggtgcgg gaacgtcgcg atggtaggag cgcatcgccc cgggacgcct
     2461 acccagcttc cgggtgtcga tcacccaggg ggaggacgga tcgggacggg cggaagcagc
     2521 atggcgcggc atgaggattt caggataaat gacgctgcgc gggcgccgaa accaagcact
     2581 accgggggta ctcgccgacc cccgccggca ccggtccgcc gtggtggctg cggccgcgcc
     2641 cgatagtgcg catcgtgcgg ttgagcagtt cctcgaactc cgccagcgtc ctgtc
//
LOCUS       DATADN010000600         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_676881, whole genome shotgun sequence.
ACCESSION   DATADN010000600 DATADN010000000
VERSION     DATADN010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676881"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VHH53_07765"
     CDS             complement(<1..746)
                     /locus_tag="VHH53_07765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2290076.1"
                     /translation="MTPSGAPVSPLEIVEQARQAEADGFPAVWTTHFSRDVDALSVLA
                     VAGTQTSRVELGVGIVPTYPRHPLALAQQAATTQALCGGRLTLGIGVSHRPVIEGLHG
                     IPYTSPAQHMQEYLSVLGPLLRKGSVTYRGSFYQVEGGFTVRDTSPVSIVVGALSPRM
                     VRVAGELADGVVTWLAGPRWLVDGIVPGLHSAAQAAGRSRPRIVAALPVAVCDDAGLA
                     RTVADDVFARPVGNTAASVGRTRALLASLNS"
     gene            complement(814..>2328)
                     /locus_tag="VHH53_07770"
     CDS             complement(814..>2328)
                     /locus_tag="VHH53_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX2290077.1"
                     /translation="VHCYRRDVVTGHAEELIDAGYALEIADAPLVQRGLTLADLAHVL
                     DLQSAGVIPQQPAGQLLGLLLDTLDIPAEEFPYDPSHGEVYNSRERYFAAGIGDAAGW
                     LHAGRPRREAMRIAFRLHLRDVLADFVGAAADLATQLAQLAGRHTETWMADQTYLQHA
                     QPTTFGHYAVSFAYPVLRDGRRLLDGLTWVDASPGGAGGVNGTALLTDRGHVAELLGF
                     GSVIEHTRDAMWQTDGLVDALAASASLLVTQGKLAEDLEIWASHEFSYVTIADGYSRS
                     SVLMPQKRNPYALTMIRGASGVAIGRLSGLLALTKTPSARSDNLIFAYGEVPRALAAA
                     VRTTRLSAGVVRTLRVHGERLWSVLEGGFTQAADLAEHLMLTYRLDYRSAYRIVGHAV
                     RTAADHGLRGVDIDAAALDAAALAVLGRSLGIDGGGVDGGALSRVLDPREVVRTRTAR
                     GGAAPAEVARMVATCTDEAGDLSRQARMRQEHFRHAEQALIAAARHAATPGTGQ"
BASE COUNT          370 a          824 c          802 g          332 t
ORIGIN      
        1 gaattcaggc tggccagcag ggctcgggtt cgcccgacac tcgctgcggt gttccccacg
       61 gggcgggcga agacgtcatc agccacggtg cgcgccaggc ccgcgtcgtc gcagaccgcc
      121 accggcaggg ccgcaacgat ccgcggtcgc gacctgccag cggcttgcgc cgccgagtgc
      181 aggccgggca cgatgccgtc caccaaccag cgcggaccgg ccagccacgt gacgacgccg
      241 tcagcgagtt ccccggccac ccgcaccatc ctcggcgaga gtgccccgac cacgatcgag
      301 accgggctgg tgtccctgac cgtgaagcct ccctcgacct ggtagaacga accccgatac
      361 gtcacgctgc ctttgcggag cagcggtccg agcacagaga ggtattcctg catgtgctgg
      421 gccgggctgg tgtaggggat cccgtgcagg ccttcgatca ccggccggtg agacacgccg
      481 atcccaaggg tcagccggcc accgcaaagc gcctgcgtgg tggccgcctg ctgagccagc
      541 gccaggggat ggcgcgggta ggtgggcacg atgcccaccc cgagctccac ccggctggtc
      601 tgagtaccgg ctaccgcaag gacactcagc gcatcgacat cgcgggagaa gtgcgtcgtc
      661 cacaccgcag ggaagccgtc cgcttcggcc tgtcgagcct gctcgacgat ctccagcggt
      721 gacaccggcg cgcccgatgg cgtcaccgcc gatacccatc gcaatgcgca tcgtctctcc
      781 acccctccca caaagcgctg cgcgaggtgc gcatcactgg ccggtcccgg gcgtcgcggc
      841 atgccgggca gcagcgatga gtgcttgctc ggcgtggcgg aagtgctcct gcctcatgcg
      901 tgcctgcctg gagaggtcac cagcctcgtc tgtgcacgtc gcgaccatcc ttgcgacctc
      961 ggcgggcgcg gcgccgccac gggcggtgcg ggtgcggacg acttcccgcg gatccagcac
     1021 ccgggacagc gcaccaccgt cgacaccgcc gccatcgata cccagcgacc ggccgagcac
     1081 ggcgagcgcg gcagcgtcga gcgcggcagc gtcgatgtcg accccgcgca gcccatgatc
     1141 ggctgcggtg cgtacggcat ggcccacgat gcggtaggcg ctgcggtagt ccaaccggta
     1201 ggtcagcatg agatgttcgg cgaggtccgc ggcctgcgtg aagccgcctt cgaggaccga
     1261 ccacagccgt tcaccgtgga ctcgcagggt ccggaccaca ccggcgctca gccgggtggt
     1321 gcgcaccgca gcggccaacg ctcggggcac ctcgccgtag gcgaagatca ggttgtcgct
     1381 gcgggccgac ggcgtcttgg tcagggcaag aagcccggac aaccggccga tcgccacgcc
     1441 ggacgcgccc cggatcatcg tcaacgcgta gggatttcgc ttctgcggca tgagcacgct
     1501 ggagcggctg tagccgtcgg cgatcgtgac gtagctgaat tcgtggctgg cccagatctc
     1561 caggtcctcc gccagcttgc cctgcgtaac gagcaggctc gcgctcgccg cgagggcgtc
     1621 gacgaggccg tcggtctgcc acatcgcgtc gcgggtgtgc tcgatcaccg acccgaaacc
     1681 gagcagctcg gcgacgtgac cgcggtcggt gagcagcgcc gtcccgttca ccccgcccgc
     1741 accgcccggg ctggcatcca cccaggtcag cccatcaagg aggcgccggc cgtcgcgcag
     1801 caccgggtag gcgaacgaca ccgcgtagtg tccgaaggtc gttggttgcg catgctgaag
     1861 gtaggtctgg tcggccatcc acgtctcggt gtgccggccc gcgagctggg cgagctgtgt
     1921 tgcaaggtcg gccgcggccc cgacgaagtc ggcgagcacg tcgcgcaggt gtagccggaa
     1981 ggcgatgcgc atggcctcgc gacgagggcg accggcgtgc aaccagcctg cggcatctcc
     2041 tattccggca gcgaagtagc gctcccggga gttgtacacc tcgccgtgcg acgggtcgta
     2101 ggggaactcc tcggcgggga tgtcgagggt atccagcagc agcccgagca gctgacccgc
     2161 aggctgttgc ggtatcaccc ccgccgactg cagatcgagc acatgggcga gatcggccag
     2221 cgtcagtccg cgttggacca gcggcgcgtc agcgatctcc agcgcgtaac cggcgtcgat
     2281 cagctcctcg gcgtgtccgg tcacaacgtc cctccggtag cagtgaac
//
LOCUS       DATADN010000601         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_816325, whole genome shotgun sequence.
ACCESSION   DATADN010000601 DATADN010000000
VERSION     DATADN010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816325"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VHH53_07775"
     CDS             complement(<1..451)
                     /locus_tag="VHH53_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290078.1"
                     /translation="MAENDLLVDVETVVGMLTGGNLPDADQIRFLLDALAAADDSALD
                     FLSGRIECHTARGGVIEPQLAALRPQARSREEKAAVALGAARAAEGAGDSATACDLLD
                     EALTLRPGLEPALHDAAQYAAARGDYVTADRYLRRSEIPSPLRPGLSE"
     gene            572..1273
                     /locus_tag="VHH53_07780"
     CDS             572..1273
                     /locus_tag="VHH53_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2290079.1"
                     /translation="MLSGVGARVLVVDDEPGVRRALERGLRAEGMQVVTATDGPSGLR
                     AALTGSFDAVLLDIMLPGLSGYRVLERLRAAGVATPVLLVSAKDGEIDQADGLDLGAD
                     GYLVKPFSFVVLVAQLRAVLRRHADQRREAVVTLGDLVIDPARRRVTWAGQPVALSPR
                     EFAVLAALARRAGSVMSKDELLHAVWGDEHAASRNAVEVYVGYLRRKLDAVGAGAVLQ
                     TVRGHGYAVGAQDRP"
     gene            1270..>1571
                     /locus_tag="VHH53_07785"
     CDS             1270..>1571
                     /locus_tag="VHH53_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX2290080.1"
                     /translation="MNRLSRLWGWIWAARPLPLRVAAVTTVAGLVVFLVLARASTAVV
                     ARALTHTLDGKLGAAVSAATPAVAAGRLAPQPLDAAVRIRVLDRAGTPVDGGGPTPL"
BASE COUNT          223 a          503 c          590 g          255 t
ORIGIN      
        1 cctcgctcag ccccggtcgc agcgggctgg gtatctcgga gcgccgcagg taccgatcag
       61 ctgtgacgta atctccgcgg gcggcggcgt actgggcggc gtcgtgcaag gctggctcca
      121 agccggggcg cagcgtcagc gcctcgtcga gcaggtcgca ggcggttgcc gagtccccgg
      181 cgccttcagc agcgcgtgca gcgcccagcg cgactgcggc cttctcctcc ctgctacgag
      241 cctgcggccg cagcgcagca agctgcggct cgatcacccc accgcgagct gtgtggcatt
      301 cgatccggcc ggaaaggaag tccagcgcgc tatcgtcggc cgcggccagc gcgtcaagca
      361 ggaaccggat ctgatccgcg tcgggcagat ttccaccggt gagcattccc acaacggtct
      421 caacatcaac gagcaggtca ttttcggcca cccccgaaac ctagctcatc gcgccagctg
      481 cctcccccgg ccgatatgga gacatatcgg ggcaacgagg cgcttcatag gcccgatatg
      541 tctccatatc gggtaggtgc cgcagggagg catgctgagt ggtgtgggtg cgcgggtgtt
      601 ggtggtcgac gacgagcctg gggttcggcg tgcgctggaa cgcgggctgc gcgccgaggg
      661 gatgcaggtc gtcaccgcaa ccgacgggcc cagcggcttg cgagcggcgt tgaccggcag
      721 cttcgacgcg gtcctgctgg acatcatgtt gccgggcctg tccgggtacc gggtactcga
      781 gcggctgcgg gcagctgggg tggccacacc cgtcctgctc gtctcggcca aggacggcga
      841 gatcgaccag gccgacggtc tggacctcgg cgccgatggc tacctggtca aaccgttctc
      901 gttcgtcgtg ctggtggcgc agctgcgcgc ggtgttgcgc cggcacgccg atcagcgccg
      961 ggaggcggtg gtcacgctgg gtgatctggt gatcgatccg gcgcggcggc gagtgacctg
     1021 ggccgggcag ccggtggcgc tgtcgccgag agagttcgcg gtactcgcgg cgctggctcg
     1081 ccgagccggc agtgtgatga gtaaggacga actacttcac gcggtatggg gtgatgagca
     1141 cgcggccagt cgcaacgccg tggaggtcta tgtcggttac ctgcgtcgca agctggacgc
     1201 ggtcggagcc ggtgccgtcc tgcagacggt gcgcgggcac ggttacgcgg tgggtgcaca
     1261 ggacagaccg tgaaccggct gagtcgtcta tggggctgga tatgggcggc ccgcccgtta
     1321 ccgctgcgag tcgccgcagt caccacggtg gcgggcctgg tggtgtttct ggtgctggcc
     1381 agggcatcca ctgcggtggt ggcgcgggcg ctgacccaca cgctggacgg caagctgggg
     1441 gccgcggtaa gcgccgccac gcccgcggtg gctgccggac gcctggcgcc gcagccactc
     1501 gacgcggcgg tccggatccg ggtgttagac cgggctggaa cacctgtcga cggcggtggg
     1561 ccgacaccgc t
//
LOCUS       DATADN010000602         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_677034, whole genome shotgun sequence.
ACCESSION   DATADN010000602 DATADN010000000
VERSION     DATADN010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677034"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VHH53_07790"
     CDS             <1..411
                     /locus_tag="VHH53_07790"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEX2290081.1"
                     /translation="RYGAAAPESASPAGRELFTLTALKHGLDRRDLPPSVSFFQGVRV
                     DPDGAFRWLGSAGPATTVDLLLHVDAIVMLANTAHPLDPRPDYTCSPLLVHAWPARAD
                     LDRLAAGELLGPLGPEHTQAIVNTEADLAARGQL"
     gene            408..1058
                     /locus_tag="VHH53_07795"
     CDS             408..1058
                     /locus_tag="VHH53_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HEX2290082.1"
                     /translation="MTTLDIPALVPGTVVLDEYVAARASWSAVVAAGDVLTIVDLAGN
                     QAVDCLLYSAVDTGVRYSAPETVRRQGRIALTTGSVLRADTGDALMTVVADEVGVHDT
                     LGGACSKESNTLRYGQHTRAQHGCLDNFLIEGSRHGLGARDMASNINWFMNVPVDPDG
                     ALGIVDGLSGPGKRVALRADIDTLVLVSNCPQINNPCNAFDPTPVRMIVTRPGAAA"
     gene            1055..>1768
                     /locus_tag="VHH53_07800"
     CDS             1055..>1768
                     /locus_tag="VHH53_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2290083.1"
                     /translation="MNHTVLIANRGEIAVRLIHAARNLGMQTVAVFSDADRGAPHVAL
                     ADRAVRIGPAAPRDSYLRTTAILDAAATTGTTIVHPGYGFLSEDADFAAAVEAAGIAF
                     AGPSPEQLRAFGTKHTARAAARAAGVPIFAGSDLLDGLDDAVTAAETIGYPVMLKATG
                     GGGGIGMQVCRNVDDLRSAYQRVVAQAERSFGSAGVFVERYVDNARHVEVQIFGDGSG
                     RVLSLGDRDCSLQRRHQKVI"
BASE COUNT          262 a          648 c          571 g          287 t
ORIGIN      
        1 cggtacgggg ccgccgcccc agagtcggca tcgcccgccg ggcgtgaact gttcaccctc
       61 accgcgctca aacacgggct cgaccgccgc gacctgcccc cgtcggtgtc gttcttccag
      121 ggcgtccgcg tcgaccccga cggtgcgttc cgctggctcg gatccgccgg tcccgcgact
      181 acggtcgacc tgctgctgca tgtcgacgcg atcgtgatgc tggccaacac cgcgcacccg
      241 ttggacccac ggccggacta cacctgttct ccgctgctgg tgcacgcctg gcccgccagg
      301 gccgatctgg atcgattggc cgccggagaa ctcctggggc cgttgggtcc tgagcacacc
      361 caggccattg tcaacaccga ggccgatctc gccgcaaggg gacaactgtg acgacactcg
      421 acattcccgc cctggtcccg ggaaccgtcg tgctcgacga gtacgttgcc gcccgcgcat
      481 cctggtcggc cgtcgtcgcc gccggggatg tgctgaccat cgtcgacctg gccggcaacc
      541 aggccgtgga ctgtctgctg tattccgcgg tggacaccgg tgtgcggtac tcggcaccgg
      601 aaactgttcg gcgccagggg cgtatcgcgt tgaccaccgg atcggtgttg cgtgccgaca
      661 ccggtgatgc gttgatgacc gtcgtcgccg acgaggtcgg cgtgcacgac actctcggtg
      721 gtgcctgctc caaggagtcg aacaccctgc gctacggcca gcacacccgc gcccagcacg
      781 gctgcctgga caacttcttg atcgaaggct cccggcacgg gttgggcgcg cgcgatatgg
      841 ccagcaacat caactggttc atgaacgtgc cggtggaccc tgacggggcg ctcggcatcg
      901 tcgacgggct gtccgggccg ggtaagcgcg tcgcgttgcg cgccgacatc gacaccctgg
      961 tgctggtgtc gaattgcccg cagatcaaca acccttgcaa cgctttcgac cccaccccgg
     1021 tgcggatgat cgtcactcgg ccgggagcag cagcatgaac cacaccgtcc tgatcgccaa
     1081 ccgcggtgag atcgcggtcc ggctgatcca cgctgcgcgt aacctcggca tgcagaccgt
     1141 cgcggtgttc tccgatgccg atcgtggtgc accgcacgtc gcgctggccg accgagcggt
     1201 ccggatcggc cccgcagcgc cccgcgacag ctacctgcgc accaccgcca tcctggatgc
     1261 cgccgccacc accggcacca ccatcgtgca tcctggctac ggattcctct cggaggacgc
     1321 cgatttcgca gcagccgtcg aagccgccgg tatcgccttc gccggcccca gccccgagca
     1381 gctgcgcgcg ttcggcacca agcacaccgc ccgcgcggcc gcccgcgccg ccggggtccc
     1441 gatcttcgcc ggctccgacc tgctcgacgg cctcgacgac gccgtgaccg ccgccgagac
     1501 catcggctat ccggtgatgc tcaaggccac cggcggtggc ggcggtatcg gtatgcaggt
     1561 gtgccgcaac gtcgatgacc tgcgatcggc ctaccagcgc gtcgtcgcgc aggccgaacg
     1621 cagcttcgga tcggccgggg tgttcgtgga acggtatgtc gacaacgccc gccacgtcga
     1681 ggtgcagatc ttcggcgacg gcagcggtcg cgtgctgtcg ctgggtgatc gggactgctc
     1741 gctgcagcgc cgccaccaga aggtcatc
//
LOCUS       DATADN010000603         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_677215, whole genome shotgun sequence.
ACCESSION   DATADN010000603 DATADN010000000
VERSION     DATADN010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677215"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..783)
                     /locus_tag="VHH53_07805"
     CDS             complement(181..783)
                     /locus_tag="VHH53_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2290084.1"
                     /translation="MPLVPRHLAPFLAAALRNPATMGAVAPSSSQLAAQMAAVVPRVG
                     EPVVVELGPGTGSVTTAIEHRLAGRGRHVAVEIDPVLAEHVRVEFPGVEVLVGDAAEL
                     ERLLAAHDVPAADAVLCGLPWSLISAAAQRAIVEVTARALRPEGSFSTLAYVHALPLS
                     PARRFRALLGEVFDEVLSTRTVWRNLPPAVTYVCRRPRQM"
     gene            complement(863..1258)
                     /locus_tag="VHH53_07810"
     CDS             complement(863..1258)
                     /locus_tag="VHH53_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO5389 family protein"
                     /protein_id="HEX2290085.1"
                     /translation="MSLDVPTTMIESAQHGELDDAAFVTVVRDSLPYAWQVISQVVGD
                     LAVNAGSEFADHAIPPPDEAARGQLLRALASDAIRGSLQRHFGVQLAFQNCHRVAAFR
                     PDAVTGEAYRRFVSARDQVLNQTPELRDC"
     gene            complement(1280..1534)
                     /locus_tag="VHH53_07815"
     CDS             complement(1280..1534)
                     /locus_tag="VHH53_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290086.1"
                     /translation="MGQSAWVRAAEQGRAPSFDALEPDVRHALDAVLAELDGVQRDWT
                     AYHRDTVHFHLRNARAWANQSAGRDLAEQVDPDFFPAVCS"
BASE COUNT          300 a          573 c          520 g          233 t
ORIGIN      
        1 ggcggctacc tgctggtcga gacgagccag cgccaggccg agcaatccgt caaaatcttt
       61 gtccgacacg ggctgatccc gcgggtggcc agttccgacg agctgaacgc caccgtcgtc
      121 atcggaacca ggcccgccct ccaggatggc aatggcagca cgctgggcca cgcctggaag
      181 tcacatctga cgcggtcgcc ggcagacgta ggtcaccgca ggtggcaggt tccgccacac
      241 cgtgcgggtg ctcagcacct cgtcgaacac ctctccgagc aaggcacgga accgccgggc
      301 cgggctcagc ggcagcgcgt gcacgtaagc gagggtcgag aaagaaccct cgggacgtag
      361 tgcgcgcgcc gtcacctcaa cgatggcacg ttgcgcggcg gcgctgatca gtgaccaagg
      421 cagcccgcag agcactgcat cagccgccgg cacgtcgtgg gcagcaagca gccgctccag
      481 ctcagcggcg tcgcctacca ggacctcgac gccaggaaac tcaacgcgca cgtgctcggc
      541 cagcaccggg tcgatttcga ccgccacgtg cctcccccgc ccagcaagcc ggtgctcgat
      601 agcggtggtg accgagccgg tgccaggtcc cagctcgacc accaccggct cgcccaccct
      661 aggcaccaca gcggccatct gagccgccag ctgtgaggag ctcggcgcca cggcgcccat
      721 agtggccgga ttgcgcaatg cggcggcgag gaacggggcg aggtgtcgcg gtaccaacgg
      781 catgcggaaa gcctgcccgc ccggagcggc aagtgtgaaa tcgatcttgc gatcaccgcc
      841 ccaaaactgg cggctcagct gctcagcagt ctcgaagctc cggtgtctgg ttcagcacct
      901 ggtcccgggc ggagacaaac cgccggtagg cctcgccggt caccgcatca gggcgaaacg
      961 ccgccacccg gtggcagttc tggaaggcca gctgcacgcc gaagtggcgt tgcaaggagc
     1021 cccggatggc gtcgctggcc agcgcccgta gcagctgacc gcgagctgcc tcgtccggcg
     1081 gcgggatcgc gtgatcggcg aactccgagc cggcgttgac cgcaaggtca ccgacaacct
     1141 gcgaaatcac ttgccaggcg tagggcaatg aatctcggac cacggtcaca aacgccgcat
     1201 catcgagctc accgtgctgc gctgattcga tcatcgtggt tggcacgtcc agtgacatcg
     1261 gttctcctca atcggtgatt cacgagcaaa cagcagggaa gaagtccggg tctacctgct
     1321 cggctaggtc ccgacccgcc gactggttcg cccaagccct cgcattgcgc aggtgaaagt
     1381 gcacggtgtc gcggtgatat gccgtccagt cccgctgtac tccgtcaagc tcggcgagca
     1441 ccgcgtccag cgcgtggcgc acgtcaggct ccaacgcatc gaacgacggt gcacggccct
     1501 gttcggcggc ccgcacccag gctgattggc ccaccgtcgc caacagggcg ctacccagtt
     1561 cgccacgcag ccaggcggtg tcatcctaga cggccgcttt gttgccgacc acccgcagcc
     1621 gtaccc
//
LOCUS       DATADN010000604         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1267644, whole genome shotgun sequence.
ACCESSION   DATADN010000604 DATADN010000000
VERSION     DATADN010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267644"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VHH53_07820"
     CDS             complement(<1..1347)
                     /locus_tag="VHH53_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2290087.1"
                     /translation="MLIRLLRTYLRPYKRPIALLVLLQFLQTCATLYLPTLNADIIDD
                     GVVKGDTGYILSYGAVMIGISLAQVVCNIGAVYYGARTAAALGRDVRAAVFDRVQSFS
                     AREVGHFGAPSLITRTTNDVQQVQMLALMTFTLVVSAPIMCVGGIVLALGLDVPLSGV
                     LVAVVPVLGICVTLIVRRLRPLFRSMQERLDTVNRVLREQITGNRVIRAFVRDEYEQQ
                     RFRKANTDLTDFALGTGNLLALMFPIVMTVVNLSSIAVVWFGAHRIDSGGMQIGDLTA
                     FLAYLMQIVMSVMMATFMFMMVPRAEVCAERIREVLDTSSSVVPPSAPVRELRRHGHL
                     ELRGAGFRYPGAEEPVLRAVDLVARPGETTAVIGSTGSGKSTLLGLVPRLFDATDGQV
                     LVDGVEVAELDPELLAKTVGMVPQKPYLFAGTVATNLRYGNPDATDEELWHALEVAQ"
BASE COUNT          267 a          584 c          483 g          218 t
ORIGIN      
        1 ctgcgccacc tccagcgcgt gccacagttc ctcgtcggtg gcgtccgggt tgccgtagcg
       61 caggttggtc gccaccgtgc ccgcgaacag atacggcttc tgcggcacca tcccgacggt
      121 cttcgcgagc agctccgggt ccagctccgc cacctcgact ccgtccacga gcacctgccc
      181 gtcggtcgcg tcgaacagcc gcgggaccag cccgagcagc gtggacttgc cgctgccggt
      241 cgagccgatg acggcggtcg tctcgcccgg ccgggccacc aggtccacgg cccgcagcac
      301 cggctcctcg gcacccggat agcggaaacc cgccccgcgc agctccagat gaccgtgccg
      361 gcgcagctcc cgcacgggcg ccgacggcgg cacgacgctc gacgaggtgt cgagcacctc
      421 ccggatgcgc tccgcacaca cctccgcgcg cggcaccatc atgaacatga aggtggccat
      481 catcacggac atgacgatct gcatcaggta ggcgaggaac gcggtcaggt caccgatctg
      541 catcccgccg ctgtcgatgc ggtgcgcgcc gaaccacacc acggcgatcg acgacaggtt
      601 caccaccgtc atgacgatcg ggaacatcag cgcgagcagg ttgccggtgc ccagcgcgaa
      661 gtccgtgaga tcggtgttgg ccttccggaa gcgctgctgc tcgtactcgt cccggacgaa
      721 ggcacggatg acgcggttgc cggtgatctg ctcgcgcagc acccggttca cggtgtccag
      781 ccgctcctgc atcgaccgga acagcggccg cagccgccgc acgatcagcg tcacgcagat
      841 ccccagcacc ggcacgaccg ccaccagcac cccggacagc ggcacgtcca ggcccagcgc
      901 cagcacgatg ccgcccacac acatgatcgg cgccgacacc accagcgtga acgtcatcag
      961 cgccagcatc tgcacctgct ggacgtcgtt cgtcgtacgc gtgatcagcg acggcgcccc
     1021 gaagtgaccg acctcacgcg cggagaacga ctgcacccgg tcgaagacgg ccgcccgcac
     1081 gtcccggccg agcgccgcgg cggtccgcgc gccgtagtac acggcaccga tgttgcacac
     1141 cacctgcgcc agcgagatac cgatcatcac ggcgccgtag ctgaggatgt agccggtgtc
     1201 ccccttcacg accccgtcgt cgatgatgtc cgcgttcagc gtgggcaggt agagggtggc
     1261 gcaggtctgc aggaactgca gcagcaccag cagggcgatg ggtctcttgt agggcctgag
     1321 gtaggtccgc aggagtcgta tgagcacgct gcttctctcg gagtggacga cggggcggtt
     1381 ggttgcccct ggcccctatc gtcgaacact ccacccgcgt tacctcaacc gaattaaccc
     1441 aacaggcggt caaaaggccc cggggtgcgt ctgctcccgc accgccacga actgctgccg
     1501 caccgcctgc cccacggcca gctcctcccc gggctccagt acctgcgcga cc
//
LOCUS       DATADN010000605         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_214907, whole genome shotgun sequence.
ACCESSION   DATADN010000605 DATADN010000000
VERSION     DATADN010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214907"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1426)
                     /locus_tag="VHH53_07825"
     CDS             complement(41..1426)
                     /locus_tag="VHH53_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX2290088.1"
                     /translation="MTTLVLALYIVSFALFIYGLMGLTGPRTAVRGNLIAAAGMGLAV
                     VATLLIPGVSNWVLIVLGVVIGTLLGVPAAKRVKMTAMPQMVALFNGVGGGAVALIAW
                     VEFRSTDGFSGEPAYVMVASLFGAIVGSVSFWGSLVAYGKLQGLLPGRPISLGKPQQV
                     VNGLALLAALACALAIGFGNDAELLIIGTLVFAAALGLLVVLPIGGADMPVVISLLNA
                     ATGLSAAAAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPAIVAGGFGGGGGT
                     APGRELGDKTVRETNAADAAVQMAYANQVVVVPGYGMAVAQAQHTVRDMAKLLEDKGV
                     EVKYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDEINGEFNRTDVALVIGANDVTNP
                     AARSDPNSPIHGMPILNVDQSKSVIVLKRSMSPGFAGIENELYYSPGTAMLFGDAKSS
                     VESLIEELKVL"
     gene            complement(1423..1749)
                     /locus_tag="VHH53_07830"
     CDS             complement(1423..1749)
                     /locus_tag="VHH53_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX2290089.1"
                     /translation="MLLANLAILVLAGFVGFAVISKVPNTLHTPLMSGTNAIHGIVLL
                     GGIIVLGLGHDLSVFDKLLAVVAIAFGTINVVGGFLVTDRMLGMFKGKQPGRAAAQGK
                     AEGEPR"
     gene            complement(1749..>2591)
                     /locus_tag="VHH53_07835"
     CDS             complement(1749..>2591)
                     /locus_tag="VHH53_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX2290090.1"
                     /translation="GSVLIGFLAPRSDPDIAAKLRDADVTAFAMESIPRISRARSMDA
                     LSSQANVAGYKAVLLAADQSTRFFPMLTTAAGTVKPATALILGVGVAGLQALATAKRL
                     GARTTGYDVRPEVADQVRSLGAKWLDLGIEAAGEGGYARELTEDERAEQKQRLTEAIS
                     GFDVVITTALVPGRKAPILVTAEAVKGMRPGSVIVDLAGETGGNCELTEPCEIVVRHD
                     VTIASPLNLPATMPEHASELYARNVQSLLELMVDEQGRLTPDSDDEVLVRSCITRQPE
                     GNHG"
BASE COUNT          439 a          872 c          851 g          429 t
ORIGIN      
        1 gcggcacgac acagcgggca tgagaacggg acacgcgggg ttagaggact ttgagctcct
       61 cgatgagtga ttcgacggag gatttggcgt cgccgaaaag catcgccgtg ccgggggagt
      121 aatagagctc gttctcgatg ccggcgaagc cggggctcat cgagcgcttg agcacgatga
      181 ccgatttgct ctggtctacg ttgaggatgg gcatgccgtg gatcggtgag ttcgggtcac
      241 tgcgtgcggc cgggttggtg acgtcgttgg caccgatcac cagcgcgacg tcagtgcggt
      301 tgaactcgcc gttgatctcg tccatttcct tgagctgctc gtagggcacg tccgcctcgg
      361 cgagcagcac gttcatgtgc ccgggcatcc ggccggccac cgggtgaatg gcgtacttga
      421 cctcaacgcc cttgtcctcg agcagcttgg ccatgtcccg cacggtgtgc tgggcttgtg
      481 ccacggccat gccgtacccg ggcaccacga cgacctggtt ggcgtaggcc atctggaccg
      541 cagcgtcggc cgcgttggtc tcccggacgg tcttgtcccc gagctcgcgg cccggtgccg
      601 tgccaccccc accgccgaac ccgccggcga cgatcgccgg gatggagcgg ttcatcgcct
      661 tggccatcag gttggtcagg atcgaacccg aagcgccgac gatcatgcca gcgacgatca
      721 tggcggtgtt gttcagcgcc aatccggctg ctgcggccga gagcccggtt gccgcgttga
      781 gcagcgagat caccactggc atgtcggcgc cgccgatcgg cagcacgacc agtagcccca
      841 gcgccgcggc gaacaccagc gtcccgatga tcagaagctc cgcgtcgttg ccgaaaccga
      901 tggcgagtgc gcaggccagc gccgccagca gcgccagccc gttaaccacc tgctggggct
      961 tacctaggct gatcggccgg ccggggagga gtccctgcaa cttgccgtac gccaccaggg
     1021 agccccagaa cgacaccgaa ccgacgatcg cgccgaacag ggaggcgacc atcacgtaag
     1081 cgggctcccc gctgaacccg tcggtgctgc gaaactccac ccacgcgatc agcgcgaccg
     1141 cgccgccgcc gacgccgttg aacagcgcca ccatctgcgg catcgcggtc atcttcaccc
     1201 gcttggcggc tgggacaccg agcagcgtgc cgatcaccac gcccagcacg atgagtaccc
     1261 agttgctcac cccagggatg agcaaggtcg ccaccaccgc gagacccatt ccggccgccg
     1321 cgatgaggtt gccacgcacc gccgtgcgcg gtccggtcaa gcccatcaag ccgtagatga
     1381 acagtgcgaa ggagacgatg tagagggcca ggaccagcgt tgtcatcgtg gctcgccttc
     1441 tgccttgccc tgagccgctg ccctgccagg ttgcttgcct ttgaacatcc ccagcatccg
     1501 gtcagtgacc aggaagccac cgaccacgtt gatggtgccg aacgcgatcg ctaccaccgc
     1561 cagcagcttg tcgaataccg agaggtcgtg gcccaggccc aacacgatga tgccgccgag
     1621 cagcacgatg ccgtgaatgg cgttggtgcc cgacatcagc ggtgtgtgca gggtattggg
     1681 taccttggag atcactgcga agccgacgaa accggccagc acgaggatcg ccaggttggc
     1741 gagcagcatc agccgtggtt tccttccggt tggcgggtga tgcaggagcg caccagcact
     1801 tcgtcatcgg agtcaggtgt caaccggccc tgctcgtcca ccatcagctc cagcagcgac
     1861 tgcacgttgc gggcgtacag ctcgctggcg tgttcgggca tcgtcgccgg caggttcagc
     1921 ggcgaggcga ttgtcacatc gtgacggacc acgatctcac atggctcggt cagctcgcag
     1981 ttgccgccgg tctcgccggc caggtcgacg atcacgctgc ctgggcgcat ccccttcacc
     2041 gcctcggcgg tgacgaggat gggtgccttg cgtccgggca ccagcgcggt ggtgatcacg
     2101 acatcgaaac cgctgatcgc ctcggtcagc cgctgcttct gctcggcgcg ctcgtcttcg
     2161 gtgagctcgc gagcgtaacc gccctcaccc gccgcctcga tgcccaggtc gagccacttc
     2221 gcgcccagcg accgcacttg atcggcgacc tcgggccgga cgtcgtagcc ggtggtcctg
     2281 gccccgaggc gcttggcggt ggccagcgcc tgcaaccctg ctacgccgac gccgaggatc
     2341 agcgcggttg ccggtttgac agtgcccgcg gccgtggtga gcatcgggaa gaagcgggtg
     2401 gactggtcgg cggccagcag caccgccttg tacccggcca cgttggcctg cgaggacagt
     2461 gcgtccatcg accgcgcgcg ggagatccgg ggaatcgatt ccatggcgaa ggcggttaca
     2521 tccgcgtcgc gcagcttcgc cgcgatgtct ggatcagatc gcggtgccag aaaaccgatc
     2581 agcaccgagc c
//
LOCUS       DATADN010000606         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_214931, whole genome shotgun sequence.
ACCESSION   DATADN010000606 DATADN010000000
VERSION     DATADN010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214931"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHH53_07840"
     CDS             <1..399
                     /locus_tag="VHH53_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2290091.1"
                     /translation="LEPIGITVEIETLDFSTWLARQDEGDYDAFMLSWLGNIDPADFY
                     QEQHITDGSSNYHGYSNPEVDRLLTEASTEVDQDARKELYDQAAQIIVDDVSYLYLYN
                     PEVVQAWVPGLEGYQIRPDRAINFEEVQLP"
     gene            408..1340
                     /locus_tag="VHH53_07845"
     CDS             408..1340
                     /locus_tag="VHH53_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2290092.1"
                     /translation="MLRRLGQSLVVLAGVSVLAFSLIHLVEGDPIRQALGTRFSQEAY
                     DALEKRNGLDLPVVQQYFRWLGHALTGDLGVSFRSGQPVTAIIAERLPATLTLAFSSI
                     LLALLIAVPLGTISALRPRSVIDRLATVFSQFGISVPDFWMAIMLILVFAGTLGWLPT
                     GGYVSPFESFGGWLQRLILPTVVTGLVSGSVITRFVRSSVLEALGQDHVRTAHAKGLP
                     PREVLNWHVLRNALLPLVTVTGVQLAYLLSGVVVVEIVFSWPGLGQLAIQAVDERNYP
                     VLQGTVLLFALVFLLINLVVDLLYTRIDPRISRR"
     gene            1294..>1509
                     /locus_tag="VHH53_07850"
     CDS             1294..>1509
                     /locus_tag="VHH53_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290093.1"
                     /translation="MTCSTPGSIRGSPADDRRRPDAGAEPRRRADAGRPSRHGQPRHV
                     RPRHVRTGHVTAGHVGSGELPAGASALA"
BASE COUNT          201 a          548 c          515 g          245 t
ORIGIN      
        1 ctggaaccga tcggcatcac cgtcgagatc gagacgctgg acttctccac ctggctggcc
       61 cggcaggacg agggcgacta cgacgccttc atgctcagct ggctgggcaa catcgacccg
      121 gccgacttct accaggagca gcacatcacc gacgggtcga gcaactatca cggctacagc
      181 aaccccgagg tggaccggct gctcaccgag gcgtccaccg aggtcgacca ggacgcccgc
      241 aaggagctct acgaccaggc cgcgcagatc atcgtggacg acgtctcgta cctgtacctc
      301 tacaaccccg aggtcgtgca ggcctgggtg ccggggctcg agggctacca gatccggccc
      361 gaccgggcga tcaacttcga ggaagtgcag ctgccctgac cggctacctg ctccggcgcc
      421 tcggccagtc gctggtggtg ctggccggcg tcagcgtgct cgcgttctcg ctgatccacc
      481 tggtggaggg cgatccgatc cgccaggcgc tgggcacccg gttctcgcag gaggcctacg
      541 acgcgctgga gaagcgcaac ggcctggacc tgccggtggt gcagcagtac ttccggtggc
      601 tgggccacgc gctgaccggt gacctcgggg tcagcttccg cagcggccag ccggtgacgg
      661 cgatcatcgc cgagcggctg ccggccacgc tgacgctggc cttctcgtcg atcctgctgg
      721 ccctgctgat cgccgtgccg ctgggcacca tctcggcgct gcggccccgc tcggtgatcg
      781 accggttggc caccgtgttc agccagttcg gcatctcggt gccggacttc tggatggcga
      841 tcatgctgat cctggtcttc gcgggcacac tgggctggct gccgaccggc gggtacgtct
      901 cgccgttcga gagcttcggc ggctggctgc agcggctgat cctgccgacg gtggtcaccg
      961 gcctggtctc cggctcggtg atcacccgct tcgtccggtc cagcgtgctg gaggcgctgg
     1021 ggcaggacca cgtccggacg gcgcacgcca agggactgcc gccgcgcgag gtgctcaact
     1081 ggcacgtgct gcgcaacgcg ctgctgccgc tggtgacggt gaccggcgtg cagttggcct
     1141 acctgctctc cggcgtggtc gtggtggaga tcgtcttctc ctggccgggg ctcgggcagc
     1201 tggcgatcca ggccgtcgac gagcgcaact acccggtcct ccagggcacg gtgctgctgt
     1261 tcgcgctggt cttcctgctc atcaacctgg tcgttgacct gctctacacc cggatcgatc
     1321 cgaggatctc ccgccgatga ccgccgccgc cccgacgccg gggccgagcc ccgacgccgc
     1381 gccgacgccg gacgcccgtc ccggcacggc cagccccggc acgtccggcc ccggcacgtc
     1441 cgcaccgggc acgtcaccgc cgggcacgtc ggctccggcg agcttccggc cggcgcgtcc
     1501 gcgctggcg
//
LOCUS       DATADN010000607         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1437227, whole genome shotgun sequence.
ACCESSION   DATADN010000607 DATADN010000000
VERSION     DATADN010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437227"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VHH53_07855"
     CDS             complement(<1..653)
                     /locus_tag="VHH53_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2290094.1"
                     /translation="MNASRSPRSTTPGIRTYVLDTSVLLSDPRAFIRFDEHEVVLPLV
                     VIGELEGKRHHAELGWFAREALRLLDDLRVTHGRLDVPVPIGELGGTLHVELNHADPA
                     VLPAGFRTETNDSRILACALNLVADGRDVTLVTKDMPLRVRAGAVGLPADEYRALDVV
                     PSGWTGMSDVEVGPSVLDELYKAGVADLTEHPDVADLPCNTGLRLLAGTATALGRITA
                     "
     gene            complement(974..2191)
                     /locus_tag="VHH53_07860"
     CDS             complement(974..2191)
                     /locus_tag="VHH53_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2290095.1"
                     /translation="MNGSATDLVREYLTLGLRFDRLVEGFVDAYTGDPALRRLVDNEP
                     RPDPATLARRARELRVELSVAGLTESRACFLDAHLAALECSGHVLAGQQVGFVAEVQA
                     YFDVLIEPGDPDVYRQAHAEIDRLLPGAGPLAQRLAEYRARDEIPPQRLEPAVHALSS
                     ALRDRVRGSVGLPAQETVYYEVVTDKPWSGFNYYLGDFRSRVAINADLGHRMANLPHL
                     VAHESYPGHHTEHCRKEAGLVRTQRQDEQTIFLVNTPQCLMAEGMADLGLRAAVGPGW
                     GPWAAEILADLGLRLDGELAERLEAAAAGLLGVRQDAALMLHDQGADPADVLAYLQRW
                     LLVTEPRARQMLRFLSDPLWRAYTTTYVEGYRLLRTWLEARPPAQPLAERYLRLLDEP
                     LVPSMVRAELAAG"
     gene            complement(2247..>2778)
                     /gene="uppS"
                     /locus_tag="VHH53_07865"
     CDS             complement(2247..>2778)
                     /gene="uppS"
                     /locus_tag="VHH53_07865"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX2290096.1"
                     /translation="DNLRNRPPDQVEPLLKIIADVVDELCAPDNPWRVRVVGALDLLP
                     PEMAQRLTTAATRTRDRSGLAVNVAVGYGGRQEIADALRKLLLKHAEAGTTMEELAEV
                     LDVEQIAEHLYTSGQPDPDLVIRTSGEQRLSGFLLWQSAHSEFWFCDAHWPAFRKVDF
                     LRALRDFAARHRRFGS"
BASE COUNT          494 a          967 c          898 g          419 t
ORIGIN      
        1 gcggtgatcc ggcccagggc cgtcgcggtt ccggcaagca gccgcaagcc ggtgttgcac
       61 ggcaagtcag caacgtcggg gtgctcggtg aggtcggcga ctcccgcctt gtagagctcg
      121 tcgagcaccg acggcccgac ctcgacgtcg ctcatcccgg tccagcccga gggcaccacg
      181 tccagcgcgc ggtactcgtc ggcaggcaag ccgaccgcgc ccgcccttac ccgcagcggc
      241 atgtccttgg taaccagcgt gacgtcacgg ccatcggcaa ccagattcag tgcacaggcc
      301 aggatccgag aatcgttggt ctcggtacgg aaaccggcgg gtagcactgc cggatcggcg
      361 tggttcagct cgacgtgcaa ggtgccaccg agctcgccga tcggaaccgg gacgtccagt
      421 cgcccgtggg tgactcgtag atcgtctagc aggcgcaacg cttcgcgggc gaaccaacca
      481 agttccgcgt ggtgccgctt gccctccagt tccccgatga ccacgagggg cagcaccacc
      541 tcatgctcat cgaaccggat aaatgcccga ggatccgaca gcaggaccga cgtgtcgagc
      601 acgtaagtac ggatacccgg cgtggtggag cggggcgagc gagaggcatt cacggcagct
      661 ccctgacgtg cgcggcaccc gcgcccgcta ctcggtgggc cggccggcct caggttgatc
      721 gctcccgcag cggcccggat agggccaccg tgacccgatt gggtcagcgg gaccgaccac
      781 gagggccggg tgccggccct cccgtgcgcg ccgtgggcgg tttatccgcc caatcgacag
      841 cagccacgat cagggcctcc cgtggcgtga cgcctccggc acgtcccgcc acttcgcacg
      901 ctaccggtgt gtggccgttg tgggcaggca tccaggcagg tgaatccagc gtgtcagccg
      961 gtgaccggca gcctcaccca gccgccagtt cagcgcgcac catcgacggg accagcggct
     1021 cgtcgagcag ccgcaggtag cgttcggcca gcggctgcgc cggcgggcga gcctccaacc
     1081 aggtccgcag caaccggtag ccctccacgt aggtggtggt gtaggcacgc cagagcggat
     1141 cggacagaaa ccgcagcatc tggcgcgccc gcggttcggt gaccaggagc catcgctgga
     1201 ggtaggccag cacgtccgcc ggatcggcgc cctgatcgtg caacatcagc gcggcatcct
     1261 ggcgaacgcc gagcaggccg gccgcagccg cctcaaggcg ttcggcgagt tcaccgtcga
     1321 gtctcagacc caggtcggcc aggatctccg cagcccatgg cccccacccg ggacctactg
     1381 cggcacgcaa cccgaggtcg gccattccct cagccatcag gcactgcggg gtgttcacca
     1441 aaaagatcgt ctgctcgtcc tgccgctgcg tccgtaccag accggcctcc ttgcggcaat
     1501 gctcggtgtg gtgcccggga taggactcat gcgcgaccag atgcggcagg ttggccatcc
     1561 gatgccccag atcagcattg attgccaccc gcgagcgaaa atcaccgagg tagtagttga
     1621 acccgctcca cggtttgtca gtgaccacct cgtagtacac ggtctcctgg gcaggcagcc
     1681 cgacactgcc gcgtacccga tctcgcagcg cactggacag cgcatgcaca gcgggttcca
     1741 gccgttgcgg cggaatctcg tctcgcgccc gatactcggc caaccgctgc gcgagcgggc
     1801 cggcgcccgg cagcaaccgg tcgatctcgg cgtgcgcctg gcggtagacg tcggggtcac
     1861 caggttcgat gaggacgtcg aagtacgcct gcacctcggc cacgaacccg acttgctggc
     1921 cggccaggac atgcccgctg cactccaacg cggcgagatg ggcatccaga aaacaagccc
     1981 gcgactcggt caggcccgcc acggaaagtt cgactcgtag ctcacgagcg cggcgggcca
     2041 gcgtcgccgg atccggacgc ggctcgttgt cgaccaagcg ccgcaacgcc ggatcaccgg
     2101 tataggcgtc gacgaagcct tcaaccagac gatcgaaccg caggcccagc gttaagtact
     2161 cgcggaccag atccgtggcg ctgccgttca tgccgctgac gatagagctc gtgagtggca
     2221 tgaagtgcta taacaccgtt tgccactcac gagccgaacc gccggtgccg ggcggcgaag
     2281 tcacgcaggg cgcgcaggaa gtccaccttt cggaaggccg gccagtgggc gtcgcagaac
     2341 cagaactccg aatgcgcgga ctgccagagc aggaaacccg acagccgctg ctcccccgac
     2401 gtgcggatca ccaggtcagg atcaggctgt ccagaggtgt agaggtgctc agcgatctgc
     2461 tcgacatcga gcacctcggc aagttcctcc atggtcgttc ccgcctcggc atgtttgagc
     2521 agcagtttgc gcaacgcatc agcgatctcc tgacggccgc catatcccac tgccacgttc
     2581 accgccaacc cagaccgatc ccgggtccga gttgctgcgg tggtcagccg ctgtgccatc
     2641 tccggcggta acaggtccag cgcgccgacg actcggactc gccacgggtt gtccggcgca
     2701 cacagctcat cgacgacatc ggcgatgatc ttcagcagtg gctctacctg gtcgggcggc
     2761 cggttgcgca ggttgtcc
//
LOCUS       DATADN010000608         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_58936, whole genome shotgun sequence.
ACCESSION   DATADN010000608 DATADN010000000
VERSION     DATADN010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58936"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1017
                     /locus_tag="VHH53_07870"
     CDS             262..1017
                     /locus_tag="VHH53_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290097.1"
                     /translation="MTGRLVLVALILILLGTGAGLAGALVLPKTYGARAEILYPIGQD
                     QQGGDPLRQDRQLSTQLVFLKSRAVLGPIAQKQGMQFKDLDKNLSVEVLQTSEVIQVE
                     ARGSTKEAARQTLQGVMDGYLALADQPSGVTRNLENQLVQTRANTARLQTQVDQFTAG
                     VLAGTATQTALNDARTQLTASLEREKAIQVRIDEINLTGRSGPNAQLLTPPYVLPDPV
                     SPQPLIAAGTGALVGLLVAGGVVAVGARRRTRP"
     gene            1028..>1555
                     /locus_tag="VHH53_07875"
     CDS             1028..>1555
                     /locus_tag="VHH53_07875"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX2290098.1"
                     /translation="MAALSLTALTDESPPTTARGSATVAGWTLVSRATGLLRVVVIGA
                     VLGPTYFANAFQAGYVVPNLVFTLIAGPVLAMVLVPGVVRAVGAGGLLRAREVFGRVS
                     GWLLAVSAGVVVLLMILAPAVAWTLTFGIPDPAQRARGLWLTTLLVLLVAPQVLLYVL
                     VHLGMAAQQARGRFAL"
BASE COUNT          233 a          500 c          547 g          275 t
ORIGIN      
        1 ggcggccagc cgaattcttt ccgttccgat gtccccgacc ttgaccgtcg agcaggttgc
       61 ccgggtctgc gatgtgctgg gaggtgcgct gtgaccaacg tcgacaatgt ctttcgttac
      121 gggggcagtg gtgagcccaa cggacaggca attgcttccc aggaggagtc gcagcagcct
      181 gcaagggctg gcgacgtgac tccgccgaag cctccgccga tccggaccga acctgcctcg
      241 gattcccgcg gtggcggtgg cgtgacaggg cgcctggtac tggtggcgct gatcctgatc
      301 ctgctcggta ccggtgccgg gctggctggc gcgctcgtct tgcccaagac ctacggagcg
      361 cgggcggaga ttctctatcc catcggccag gaccagcagg gcggggatcc gctgcgccag
      421 gatcgccagc tcagcaccca gctggtgttc ctcaagagcc gggctgttct cggtccgatc
      481 gcgcagaagc agggcatgca gttcaaggac ctggacaaga acctgagcgt ggaagtcctg
      541 cagactagcg aggtcatcca ggtcgaggcg cgcggcagca ccaaggaggc tgcgaggcag
      601 acgctgcagg gagtcatgga cgggtacctg gcgcttgctg accagcccag cggagtaacc
      661 cgcaacctcg aaaatcagct cgttcaaaca cgcgcgaaca ctgctcgtct tcagacacaa
      721 gtggatcagt tcacggctgg ggtattggcc gggaccgcga cccagacggc cctcaacgat
      781 gcccggacac agctcaccgc atccctggaa cgggagaagg cgatccaggt ccggatcgac
      841 gagatcaacc tcaccggcag gtcaggaccg aacgcacagc tgctgacacc gccttacgtg
      901 ctgcccgatc cggtgagccc gcagccgctg atcgcggccg gtaccggtgc gctggtcggg
      961 ctgctggtgg ctggcggggt tgtggcggtg ggtgcccggc gccggacgag gccgtgaggg
     1021 ccgggctgtg gccgccctgt cgctaacagc actcaccgac gaatctcccc ccacaacagc
     1081 gcgcggctcg gcgacggttg ccggctggac gctggtcagc cgggcgaccg gactgctccg
     1141 ggtggtggtg atcggcgcgg tcctgggccc cacctacttc gccaacgcct tccaggccgg
     1201 ctatgtcgtc cccaacctcg tgttcacgtt gatcgccggg ccggtgctcg cgatggtgct
     1261 ggttcctggt gtggtccgcg ctgtgggtgc cggtggcttg ttgcgggctc gagaggtgtt
     1321 cggtcgggtc agcgggtggt tgctcgcagt gtcggccggg gtcgtcgtgt tgctgatgat
     1381 cctcgctccc gcggtggcct ggaccctgac cttcgggatc ccggaccccg cgcaacgggc
     1441 tcggggactg tggctgacca cgttgctggt gctgcttgtc gcaccgcagg tgctgctata
     1501 cgtcctggtg cacctgggca tggccgctca gcaggcccgc gggcgcttcg cgctc
//
LOCUS       DATADN010000609         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_59021, whole genome shotgun sequence.
ACCESSION   DATADN010000609 DATADN010000000
VERSION     DATADN010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59021"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHH53_07880"
     CDS             complement(<1..295)
                     /locus_tag="VHH53_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290099.1"
                     /translation="MTHVVGPPPTAATVAEELADRKPTSVMRARSWGVPKIAGLFGGF
                     GALFAVLFALAPVDQRMATYSWDLDGVVSATPIPLSSYRPIAMSVTFGCAQVNA"
     gene            complement(292..687)
                     /locus_tag="VHH53_07885"
     CDS             complement(292..687)
                     /locus_tag="VHH53_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290100.1"
                     /translation="MPNRIATDTGCRRMGWTIAVLALLTGVLGNLGLRAGQQPTSTVT
                     PLSPYRPSLGPLPLRNPLLWLAVAGLSYWMTIRSRRWPGPDRAVPAVRRAVCCDVKIL
                     SAVHLAGGAWGIGTAIAPNPSKPRRGCGV"
     gene            1014..3017
                     /locus_tag="VHH53_07890"
     CDS             1014..3017
                     /locus_tag="VHH53_07890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX2290101.1"
                     /translation="MLTPFQPSAPVDTAGRTARYRPELQGLRALAVALVVVYHVWFNR
                     VSGGVDVFFLVSGFLLTGQLVRAADRGPIALRPLWGRMATRLLPVALTVLLATVVAAV
                     LVLPEGRWFQTIREVAASALLLQNWQLAADAVDYAAQNNTASVVQHFWSLSIQGQVFV
                     AWPLLVVLVVLIARRAGADLREYLALALVGVGVTSFSYSVALTAADQPLAYFHTLTRL
                     WEFAFGGLLALGVDRVSLSRATRTVLGWTGAVGLLLCGLVLQVGSVFPGYAALWPTTC
                     AALVIAAGVTGSRWGADRLLAARPAQYLGDLSYALYLWHWPVLVLYLVGRGRDAVGLR
                     GGVLIIGTSLVLAALTHHLVERPALRRAGPRPYRALAAGTALVLLTVAGWQIAAVHRI
                     TDVGRPGDSDHPGARALAGAVADPAVEPLPPMVSVYEDWVRIDHWDCAPMARFPMDVC
                     TQPVEYQPERRIVVVGDSHALQFAGALVPLAQQRGWQLISMVRGACPFSTASEVVFDD
                     PDCLAWNGAAAAEIADLRPDGVVTLASRDVRTGLTEVTPPGFVEQWWRLHDLSIPVLA
                     VRDNPRFDFSPPDCLQHLGRSAPECGVERAAVYAPEPPWARLNVPPNVSFLDLSDQLC
                     NAVHCPAEIGNVLVYMDDNHLSDSYAATMAPAVERHVAVTLGW"
BASE COUNT          499 a         1210 c         1257 g          612 t
ORIGIN      
        1 gcgcgttcac ctgagcacat ccgaacgtca ctgacatcgc gatgggccgg tagctgctca
       61 gtgggatcgg cgttgcgctg accacgccgt cgaggtccca gctgtaggtt gccattcgct
      121 gatcgaccgg ggcgagcgcg aataacaccg cgaacagcgc cccaaacccc ccgaacagcc
      181 ccgcgatctt gggtacgccc caggacctgg cgcgcatcac cgaggtcggc ttccgatcgg
      241 cgagttcctc cgcgacggtc gctgcggtgg gcggcggccc gaccacatga gtcatactcc
      301 acacccccgc cgcggcttac tcgggttggg agcgattgcg gtgccgatgc cccaggcacc
      361 accagcgagg tgcacggcac tcaggatctt cacatcacaa caaacggccc gtctcaccgc
      421 cggcactgcg cggtctggtc ctggccagcg ccgagaccgg atcgtcatcc aatagctcag
      481 ccctgcgact gccaaccaga gcagcgggtt gcgcaacggc agcggaccca gcgacggccg
      541 gtacggcgac aatggagtta ccgtcgaggt gggctgctgc ccggcgcgga gcccgaggtt
      601 ccccagcacg ccggtgagca gcgccaacac cgcaatcgtc caccccatcc ggcgacaacc
      661 cgtgtcggtg gcaatacggt tcggcaccgc ggcaggctat ggggccgggc gcgcctggct
      721 agcggtgggt ggtgtggtca gccgaaccgg ctcagcgccg cagggcgttg gtcagggtca
      781 tcggcgcgga tcgatgatcg tggtagtcgg aactcgggac tgcgccggac cggtcgagcc
      841 ctgaccgatt gcttttaccc gcttctgacc gagtgcggcg gctcggacgc cggctgttca
      901 tctctcgcag cgtggtcatc tggtgtcggt aggtaatggg cacctgaaag aatcaaaccg
      961 cggctagccc ggggcggggg cgagaacgag cggggaggag gaccgagcgc aggatgctaa
     1021 ccccgtttca gccgtccgct cctgtagaca cggctggccg caccgcgaga tatcgaccgg
     1081 agctgcaggg acttcgggcg ctcgcggtgg ccctcgtcgt cgtctaccac gtgtggttca
     1141 accgcgtgtc tggtggcgtg gacgtgttct tcctcgtgtc cgggtttctg ctcaccggcc
     1201 agctggtgcg cgccgccgat cgtggcccga tcgcgctgcg gccgctgtgg ggtcgcatgg
     1261 ccacccggct gttgcccgtg gctctcacgg tgttgcttgc caccgtcgtc gccgcggtcc
     1321 tggtcctgcc ggagggacgg tggtttcaga ccatccgtga ggtcgcggcg tcggcgctgc
     1381 tcctgcagaa ctggcagctc gctgcggacg cggtcgacta cgcggcgcag aacaacacgg
     1441 ccagcgtggt gcagcacttc tggtcgctgt cgatccaggg gcaggtcttc gtggcgtggc
     1501 cgctgctggt ggtgctcgtc gtgctgatcg cccgccgcgc cggcgccgac ctgcgcgagt
     1561 atcttgccct ggcgctcgtc ggggtcgggg tgacctcatt cagctactcg gtcgcgctga
     1621 cggccgccga ccagccgctg gcgtactttc acacgctcac ccggctgtgg gagttcgcgt
     1681 tcggtgggtt gctcgcgctg ggggttgacc gggtctcgct gtcccgggca acccggacgg
     1741 tgctgggctg gaccggcgcg gtgggcctgc tgttgtgcgg gctcgtgctg caggtcggca
     1801 gtgtctttcc cggttacgcg gcgctgtggc caacgacgtg cgccgcgctg gtgatcgccg
     1861 ccggcgtcac gggcagccga tggggcgccg accggctgct agccgcccgg ccggcgcagt
     1921 acctcgggga tctcagctat gcgctgtacc tgtggcactg gccggtcctg gtgctctacc
     1981 tcgtcggccg ggggcgggat gcagtcggct tgcgcggcgg tgtgctcatc attggcacgt
     2041 ccctcgtgct cgccgccctc acccaccacc tggtcgaacg gccggcgctg cgtcgggccg
     2101 gaccgcggcc ctaccgtgcc cttgcggcgg ggacggcgct ggtgctgctc actgtcgctg
     2161 gatggcagat cgcggcggtg catcggatca cggacgtcgg ccgccctggc gactccgacc
     2221 atccgggcgc gcgggctctg gccggtgccg tggcggatcc cgcggtcgag ccgctgccgc
     2281 cgatggtctc ggtgtatgag gactgggtgc gcatcgacca ctgggactgc gcgccgatgg
     2341 cacggttccc gatggacgtg tgcacacaac cggtcgagta ccagcccgag cgaaggatcg
     2401 tcgtggtcgg cgactcacac gcgctgcagt tcgcgggcgc gctggtgccg cttgcccagc
     2461 agcgcggctg gcagctgatc tccatggtgc gtggagcctg cccattctcc accgcctcgg
     2521 aggtggtttt cgacgatcca gactgcctgg cgtggaacgg cgcggcggct gcggagatcg
     2581 cggacctgcg gcctgacggc gtcgtcaccc tggccagccg cgacgtgcgg accgggctca
     2641 ccgaggtcac cccgcccggc ttcgtcgagc agtggtggcg gctacacgac ctgagcattc
     2701 ccgtgcttgc cgtgcgcgac aacccgcgct ttgacttctc cccacccgac tgcctgcagc
     2761 acctcggtcg gtctgcaccc gagtgcgggg tcgagcgcgc cgctgtctac gcgcccgagc
     2821 cgccatgggc gcggctgaac gttccgccca acgtgtcgtt ccttgacctg tccgatcagc
     2881 tttgcaacgc ggtgcactgc ccggccgaga tcggcaacgt gctggtgtac atggacgaca
     2941 accacctcag cgacagctac gccgccacca tggcgcccgc tgtcgagcgg cacgtggctg
     3001 tcacgctcgg ctggtgagtg gcaagccggg tacaagtgga cgtggctccg tgcggccctg
     3061 agggctgcga gaagtgggga ccaggcccca tggacagatg gacaccgcga ggcgacgctg
     3121 tttaatcctg ctggtcgacg accacgctag tgcggcacgg tctgcgttta attctgaacg
     3181 gcgaaccgga cttcgtcgtc gtcgccgagg ccggtgatgg cggtcatccg cacagcgaag
     3241 ccgccgccca cgcaccagag ccgctaggcc agccccacaa ccgctcggtc cgagagatcg
     3301 gctacgcgag tttcccgcac ggggtgtgcg tgcacgtgcc ggggtatccg tgcagcatgt
     3361 agttcaaccg ccgcggggct gtcgtcgcga agcaccgatc gaacgccggc ccgccgtaag
     3421 gggtaaattc gtgctggctg cgcggggctg ggtatgcgac cgtgaagtat gcgacccggg
     3481 atcacgacga tgttgtccgt gggtgtcgcg ctaatcgccg cagcggcgat gggtgctcct
     3541 gctgccaccg caaccacggc caccgcagcg caagaaac
//
LOCUS       DATADN010000610         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_384533, whole genome shotgun sequence.
ACCESSION   DATADN010000610 DATADN010000000
VERSION     DATADN010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384533"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHH53_07895"
     CDS             complement(<1..192)
                     /locus_tag="VHH53_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2290102.1"
                     /translation="MDEDQYDVIVVGARCAGSSTAMLLARYGYRVLVVDRATFPSDTI
                     STHVIHPPGVAALQRWGLLD"
     gene            complement(447..>1668)
                     /locus_tag="VHH53_07900"
     CDS             complement(447..>1668)
                     /locus_tag="VHH53_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290103.1"
                     /translation="ELRGAVNGSPRQLGNGNGAWPLPDGGDQELPCDQGGPCLDDLIQ
                     AHPHSRTRGSREDFARLLAQYRGTNHEIVDHYFAKHIQAGALHMCRRTRFRRRRDFEI
                     RMRYPGDIVAAVQPEFEAALWRARAMEQQTALLVAGRPRRVLLEMLFSVIVYLLGVLD
                     SVECLAATGQGARKTEECSRLHRIEKALEIAQGELTRLQEFADQAAVRAALKHYVLGL
                     FIGGIAVIASAWTASQVQMFEQAPTLDLVHAIAAGGLGAIISVLIRISRGQRLDVDTT
                     QEPFLTCLVGSSRTVIGGALGVALYVLLKAEFIPLDIPAPSDATAPYFFTAIAFLAGF
                     SERWAQDTIVRSTPLATSASGSPIPSTSTPCSSVPTETAGGVTGTGLMRTTNLPAIAP
                     TTPDEADLTRRPPE"
BASE COUNT          248 a          568 c          586 g          266 t
ORIGIN      
        1 gtcgaggagg ccccagcgct gcagcgccgc cacccccggg ggatggatga cgtgcgtgga
       61 gatcgtgtcg ctggggaagg tcgcgcggtc cacgaccagg acccggtagc cgtagcgcgc
      121 cagcagcatc gcggtggacg acccggcaca ccgcgcgccg acgacgatga cgtcgtactg
      181 atcctcgtcc atgtcgcacc gcccaggggg cagaggctgg gactgccgga cgtagcgggt
      241 ccgtacgcgt ggaactgtac cgccggacca cgctgtagct gatccggtga gcgcgcccgg
      301 caggaccgga ccgtgcgacc gggagatcgt tcctcagcgc tgggtcggct tcgcctggct
      361 gcacgcctcc acgcgcccag gtcggtgtgc tgagtcggat ggacgtgccg aggcagccgg
      421 ccacgccgat ccgcgtaccc gcgcgatcac tcgggaggtc gccgagtcag gtcggcctcg
      481 tcgggcgtgg ttggcgcgat cgccggcagg ttcgtcgtcc gcatgaggcc tgttcccgtc
      541 acgccacctg ctgtctcggt cggcacagag gagcacggtg tgctcgtcga cgggatggga
      601 gaaccactcg ctgacgtcgc cagcggcgtg ctccggacga tggtgtcctg ggcccaacgc
      661 tcgctgaagc ctgccaggaa cgcgatggcc gtgaagaagt agggcgccgt ggcgtcggac
      721 ggcgctggga tgtcgagcgg gatgaactcc gccttcagca gtacgtacag cgccacaccg
      781 agcgcgccgc cgatgacggt gcgggagcta cccaccaagc aggtcaggaa cggttcctgc
      841 gtggtgtcca cgtcgagccg ctggccacgg ctgatccgga tcagcacgct gatgatggca
      901 cccagtccac cggctgcgat cgcatgcacc aggtccagcg tcggcgcctg ctcgaacatc
      961 tgcacctggc tggccgtcca tgctgacgcg atgacagcga tcccgccgat gaagagaccc
     1021 agtacgtagt gcttgagggc ggccctgacc gccgcttgat cggcgaactc ctgcaggcgc
     1081 gtcagttcgc cctgcgcgat ctccagggcc ttctcgatgc ggtgcagacg actgcactcc
     1141 tcggtcttcc gcgctccttg tcctgtggct gcgaggcact cgacggagtc caggaccccg
     1201 agcaggtaca cgatcaccga gaagagcatc tcgaggagca cgcggcgggg gcgccccgcc
     1261 accaggagcg cggtctgctg ctccatcgcg cgtgcccgcc agagcgcggc ctcgaactcg
     1321 ggctgcacgg cggcgacgat gtcgccgggg tacctcatgc ggatctcgaa gtccctccgc
     1381 ctgcggaacc gagttctccg gcacatgtgc agcgctccgg cctggatgtg cttcgcgaag
     1441 tagtggtcga cgatctcgtg gttggttccc cggtactgcg cgagcagcct cgcgaagtcc
     1501 tccctggagc ctctggtcct ggagtggggg tgggcctgga tgaggtcgtc gaggcagggg
     1561 ccgccctgat cgcacgggag ctcctggtcg cccccgtcgg gcagcggcca ggccccgttc
     1621 ccgttcccca gctgccgtgg cgatccgttg acagcccctc gcagttcc
//
LOCUS       DATADN010000611         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_678427, whole genome shotgun sequence.
ACCESSION   DATADN010000611 DATADN010000000
VERSION     DATADN010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678427"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VHH53_07905"
     CDS             complement(<1..441)
                     /locus_tag="VHH53_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2290104.1"
                     /translation="MPVVALNGIRLSYQVTGCGELVVLVMGTGSPGRVWQLHQVPALV
                     AAGYRVATVDNRGIAPSDECASGMTIDDLVGDTAALIEHLGDGPARVVGTSLGSRVVQ
                     ELALARPDLVSHAVMMAAHGRPDPVQSRLSAGERALHDAEIELPS"
     gene            complement(669..>1564)
                     /locus_tag="VHH53_07910"
     CDS             complement(669..>1564)
                     /locus_tag="VHH53_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2290105.1"
                     /translation="VRELIKDYGRKRAVNRLSFTARAGVVTGLLGPNGSGKSTTMRII
                     LGLDAPTSGIALVNGQRVAAIDRPMRTVGAPLDAGATHGECSAADHLRCLARGHGIDR
                     RRVAEALDQVGLLPMADTLIGSLSPGVRQRLGIAGALLGDPGVLLLDEPLDGLGPEGI
                     WWIRDLTRSLAAQGRTVLVSSRHMSQMALIADQLLITSRGELITETSTRELTERSQRD
                     VFVRSPRRSGLTKRLTSMGATVLAEPSGGLSVIGMDAWRIAAVAAEHYIPIQELTPRI
                     TSLEDYYAKLTSAKSDYRVHP"
BASE COUNT          286 a          554 c          471 g          253 t
ORIGIN      
        1 cgacggcagc tcgatttccg cgtcgtgcag agcgcgctct cccgccgaca gtctggactg
       61 cacggggtca ggccgcccgt gcgcagccat catcactgcg tgtgacacaa gatccggacg
      121 cgccaacgcc aactcctgca caacccggga gcccaacgaa gtaccgacta cccgcgctgg
      181 cccgtcaccc aggtgctcga tcaacgccgc ggtgtcaccc accagatcat cgatcgtcat
      241 tccgctggcg cactcatccg agggcgcgat gccacggttg tccacggtag ccacccggta
      301 gccggccgcg accaacgccg gaacctgatg tagctgccac acccggccag ggctgcccgt
      361 ccccatcacc agcaccacta gctcaccgca gccggtcacc tggtagctga gccgaatccc
      421 attgagcgcg acaaccggca tgatcctcct tgtcaccaac tccgagcaga ccaatttacg
      481 gcgcttcagc ccaaatgatg cgctcaaatt agttgggccg tcgacgatga gcacgcagcc
      541 agcggtccgc gtggcgatgc aagagtgaca ccgaccagac atagccggtg cggcggtact
      601 caggatcgca ctatccacgc agtgctcgac cggcacgtag ccggaggccg ctgacccggc
      661 tccgtcgttt agggatgcac gcgatagtcg ctcttggcgc tggttagttt tgcgtagtag
      721 tcctcgagtg acgtgatgcg tggggtcagc tcctggatcg gtatgtaatg ctcagcagcg
      781 accgccgcga tcctccatgc gtccatgccg atgaccgaca gcccaccgct gggctcggcg
      841 agcaccgtgg cacccatgct ggtcagcctt ttggtcagcc cgctgcgacg gggtgagcgc
      901 acgaacacgt cacgttgaga acgctcggtc agctcccgcg tggaggtctc cgtgatcagc
      961 tcgccacggg aggtgatgag gagctggtcc gcgatcagtg ccatctgact catgtggcgg
     1021 ctagatacca gaaccgtccg gccctgagcg gccagcgacc tcgtcaggtc gcggatccac
     1081 cagatgccct ccggacccag tccatccaac ggctcgtcga gcaacaggac accggggtcg
     1141 ccgagcagcg caccggcaat cccgagccgc tgccggacgc cgggggacaa gctgccaatc
     1201 agcgtgtcag ccattggcaa aagccctacc tggtcgagcg cctcggccac ccgacgccgg
     1261 tcgatgccat gaccgcgcgc caggcaccgc aggtggtccg ccgcgctgca ttcgccgtgc
     1321 gtggcgcccg cgtccagcgg cgctcccaca gtgcgcatcg gtcgatcgat ggccgcgaca
     1381 cgctgcccgt tcaccagtgc tatgccagac gtcggagcat ctaagccgag gatgatccgc
     1441 attgtggtcg acttcccgga accgttcggg ccgagtaagc cggtcaccac ccccgcacga
     1501 gcggtgaatg acagccggtt caccgcacgc ttgcggccgt agtccttgat cagctcgcgt
     1561 acct
//
LOCUS       DATADN010000612         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_531397, whole genome shotgun sequence.
ACCESSION   DATADN010000612 DATADN010000000
VERSION     DATADN010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531397"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VHH53_07915"
     CDS             <1..141
                     /locus_tag="VHH53_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX2290106.1"
                     /translation="PVELPGHGPQLRALTRGAEQAGPAESAANPRAASVRLRAAERIA
                     PS"
     gene            138..764
                     /locus_tag="VHH53_07920"
     CDS             138..764
                     /locus_tag="VHH53_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HEX2290107.1"
                     /translation="MTAPPRTVAATRSKATAPARRRETGTQRMRSRAVQRAYTRRAQR
                     AATPHGGRHSGDSRWVDPHSVDSHRARFVLLMMVLLAVALVATLWLSTAAAADSYHLQ
                     SAQERARNLTERSENLSREVANLETAPELARRARGLGMVPAGDPAQLVVRPDGAVVLI
                     GQPRRATAPAPPQAPAPAAAPAPAEPPPPAAAEPPAPAPAAPAPPGGT"
     gene            845..>1943
                     /locus_tag="VHH53_07925"
     CDS             845..>1943
                     /locus_tag="VHH53_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2290108.1"
                     /translation="MALVFALVAAGLKLVQVQGLQAAELSARAANQRTTVQTLPAERG
                     TITDRNGTPLAFSVQAMALYAQPNRIAAEQRAVGADPDSHKLAMAHRIAQVLGPPVTE
                     QEILSQLRSDRTTVLLAPLVQPAQARTIQQEFPEVSSEHRETRQYPGGELAANVLGVA
                     NWRADERKVRGLVGLENYADTLLAGQDGRRIVETAEGSDAIIPGSERGVRPPVPGSGL
                     ELTLVSDLQFTLQQMLGDFARKYRAKGASAVVLDAHSGEVYAMANEVTFDPTNLAAAT
                     PELLANPAVSAPFEPGSVNKVVTVAAAIENGVLQPESALRVPGHLRVADRTVSDAWSH
                     STLDLTFTGVLARSSNVGTLLAAQRVGEDRYV"
BASE COUNT          302 a          702 c          659 g          280 t
ORIGIN      
        1 ccggtcgagc tgcccggtca cgggcctcag ctgcgcgcgc tgacccgcgg cgccgagcag
       61 gccggcccgg ccgaatccgc agccaatccc cgggcggcct cggtgcggct gcgggccgct
      121 gaacggatcg cgccctcgtg acagctccac cgcgcaccgt ggctgcgacg cgcagcaagg
      181 ccaccgcacc cgctcgccgc cgggaaaccg gcacgcagcg catgcgttct cgagcggtcc
      241 agcgggctta cacccggcgc gcacagcgcg cggccacacc ccacggtggc cggcacagtg
      301 gtgactcccg ctgggttgat ccccacagtg ttgactccca tcgggcgcgc ttcgttcttc
      361 tgatgatggt gctgctggcg gtggcgctgg tggccacgct gtggttatcc acggcagccg
      421 ccgcagattc ctatcatctg cagagcgctc aagagcgtgc caggaacctc acggagcgtt
      481 cggagaactt gagccgcgag gtcgcgaatc tggagactgc ccctgagttg gctcgccgcg
      541 ctcgtgggtt gggcatggtg ccggccgggg atccggccca gctggtggtc cgcccggacg
      601 gcgccgtcgt gctgatcggc cagcctcggc gagccaccgc gcctgccccg ccccaggcgc
      661 ccgccccggc tgcagcaccc gcacccgctg agccaccacc accggctgcc gctgagccac
      721 cagcacccgc acctgcagca ccggcaccgc ccggcggtac ctaatggaat cacgtcgtcc
      781 gcgtcgtcga cacagacgcc gcgcatcggc aggcagcctg cgaaaccggc tggtggtggg
      841 gcgcgtggcg ctcgtgttcg cgctggtggc cgctggcctc aagctggtcc aggtgcaagg
      901 cctgcaggcg gcggagctct cagcacgagc cgctaaccaa cgcaccacgg tccagacgtt
      961 gcccgccgag cgcggcacga tcactgatcg caatggcacg ccgctggctt tcagcgtgca
     1021 ggcgatggcc ctttatgccc agcccaaccg gatcgctgcc gagcagcgcg cggtcggtgc
     1081 cgatccagat tcgcacaagc tggcgatggc ccaccggata gcccaggtgc tcggtccgcc
     1141 ggtgaccgag caggagatcc tcagccagct gcgcagtgac cgcaccacgg tgttgctggc
     1201 gccgttggta caacccgccc aggccaggac gatccagcag gaattcccgg aggtcagctc
     1261 ggaacaccgg gagacccgac agtatcccgg cggcgagctc gcagccaatg ttctcggcgt
     1321 ggcgaactgg cgtgccgacg agcgcaaagt gcgcggattg gtcggcctgg agaactacgc
     1381 cgacaccctg ctggccggcc aggacggtcg ccggatagtg gagaccgcag agggctcgga
     1441 tgcgatcatc ccgggcagcg agcgaggtgt ccggccgccg gtacccggct cgggactgga
     1501 gctcaccctc gtttctgacc tgcagttcac cctgcagcag atgctcggcg acttcgcgcg
     1561 gaagtaccgg gccaagggcg ccagcgccgt ggtgctcgac gcacactccg gcgaggtcta
     1621 cgccatggcc aacgaggtca ccttcgatcc gaccaacctc gccgcggcga ctcctgaact
     1681 gctggccaac ccagcggtgt cggcaccctt cgagcccggt tcggtgaaca aggtggtcac
     1741 ggttgccgcg gctatcgaga acggcgtgtt gcagccagag agtgcgctga gagttcccgg
     1801 ccacctccga gtcgccgacc ggacggtctc cgacgcgtgg tcacacagca cgttggatct
     1861 caccttcacc ggggtgctgg cccgctcgtc caacgtgggc acgctgctcg ccgcgcaacg
     1921 ggtcggggag gaccgctacg tgg
//
LOCUS       DATADN010000613         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1268675, whole genome shotgun sequence.
ACCESSION   DATADN010000613 DATADN010000000
VERSION     DATADN010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268675"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..713
                     /gene="sufC"
                     /locus_tag="VHH53_07930"
     CDS             21..713
                     /gene="sufC"
                     /locus_tag="VHH53_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX2290109.1"
                     /translation="MLRGVDLTVRAGETHAMMGPNGSGKSTLAYSIAGHPRYQVTSGQ
                     VLLDGADVLAMSVDERARAGLFLAMQYPVEVPGVSMTNFLRTAATAIRGEAPKLRTWV
                     KEVKSAMSELDIDPSFAERSVNEGFSGGEKKRHEILQLSLLKPKIAVLDETDSGLDVD
                     ALRVVSEGVNRYRANGDVGVLLITHYTRILRHITPDHVHVFAGGRIVESGGPELAEEL
                     EAGGYERFAVRT"
     gene            728..>1549
                     /locus_tag="VHH53_07935"
     CDS             728..>1549
                     /locus_tag="VHH53_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX2290110.1"
                     /translation="MAEPLTASELEKIRADFPILSRTVREDRPLVYLDSGATSQRPRQ
                     VMDAERAFLEQHNAAVHRGAHQLAEEATDAYESARARIAAFVGIQPGEVVFTKNATEG
                     INLVAYALSNSTVAGGAAQRFAVAPGDEIVVTEMEHHANLVPWQELCRRTGATLRWYR
                     VTDAGRLDLDSLELSERTKVVAFTHQSNVMGTVPPVAELVRRAREVGALTVLDACQSV
                     PHTPVDFAALDIDFAVFSGHKMLGPSGVGVLYGRRELLEALPPFLTGGSMIELVRM"
BASE COUNT          258 a          495 c          539 g          257 t
ORIGIN      
        1 aacagactcc tgacaaggag atcctacgcg gcgtcgacct gaccgtccgg gcaggggaga
       61 cccacgcgat gatgggcccg aacgggtcgg gcaagtccac cttggcctac tcgatcgctg
      121 ggcatcctcg ataccaggtc acctccgggc aggtgctgct tgacggcgcc gacgtgctag
      181 cgatgtcggt cgacgaacgt gcccgcgcgg gcctgttcct ggcgatgcaa tacccggtcg
      241 aggtacccgg tgtgtcgatg acgaactttt tgcgcaccgc ggccaccgcg atccgtggtg
      301 aggcgcccaa gttgcgcacc tgggtcaagg aggtcaagtc ggcgatgtcc gaactggaca
      361 tcgacccttc cttcgccgag cgcagcgtga acgagggctt ctccggcggt gagaagaagc
      421 gccacgagat cctgcagttg tccctgctca agcccaagat cgcggtactc gacgagaccg
      481 actccggttt ggacgtggat gcgctgcggg tggtatccga gggtgtcaac cggtaccggg
      541 ccaacggaga cgtcggggtg ctgctgatca cgcactacac ccggatcctg cgacacatca
      601 cgcccgacca cgtgcacgtg ttcgcgggcg ggcggatcgt ggaatccggc gggccggaac
      661 tcgcagagga attggaggct ggcggctatg agcgcttcgc cgtcaggacg tgacaggggt
      721 aaccattgtg gctgagccac tgactgccag tgagttagag aagatccgcg ccgacttccc
      781 gatcctgtct cgcacagtgc gggaggaccg accgctggtg tacctggact ccggagccac
      841 ctcgcagcgg ccccggcagg tgatggacgc cgagcgcgcc ttcctcgagc aacacaatgc
      901 agcggtgcac cgcggcgcgc accagctcgc cgaggaggcc accgacgcct acgagtcggc
      961 gcgggcccgg atcgcggcgt tcgtcggtat ccagccgggg gaggtggtgt tcaccaagaa
     1021 cgccaccgag gggatcaacc tggtggccta cgcgctgagc aattcgacgg tcgccggggg
     1081 agcggcgcaa cgctttgccg tggcgccggg tgacgagatc gtcgtgaccg agatggaaca
     1141 ccacgccaac ctggtgccct ggcaggaatt gtgccggcgt accggcgcca cgctgcgctg
     1201 gtaccgggtg accgatgctg gccgcctcga cctggactcc ttggagctgt ccgaacgcac
     1261 caaggtggtg gccttcaccc accagtccaa cgtgatgggc accgtcccgc cggtcgcgga
     1321 gctggtccgt cgagctcgcg aggtcggtgc gttgaccgtg ctggacgcct gccagtcggt
     1381 gccgcacacg ccggtcgact ttgctgcgct ggacatcgac ttcgcggtgt tctccgggca
     1441 caagatgctg ggaccgtccg gggtcggcgt gctctacggc cgccgtgaac tgcttgaggc
     1501 gctgccgccg ttcctcaccg gcgggtcgat gatcgagctg gtgcggatg
//
LOCUS       DATADN010000614         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_817917, whole genome shotgun sequence.
ACCESSION   DATADN010000614 DATADN010000000
VERSION     DATADN010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817917"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="VHH53_07940"
     CDS             <1..1241
                     /locus_tag="VHH53_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2290111.1"
                     /translation="SVGEDDFWPRGAGRNPWRDPYAAAVLGAPAVAEHHRDPGDPSRP
                     GARLSLQEALFGRQLSNRALALLCAVVLLIGGVGGFVGRLTADGGNLLTDPGATLAQV
                     EPATDRSAGSVAAVASRVLPAVVSLEFRVGDQGGFGSGVVIDGQGYLLTNNHVVVPAA
                     TTSQATLEAVFSDGSRVPARIVGRDPKTDLAVVKVEVANPTVAQMGRSSTLKPGDSVI
                     AIGSPLGLAGTVTSGIVSAVDRPVRLEGDGSHGNAVIDAIQTDAAINPGNSGGALVNT
                     AGAVVGINTAIRTLGAREGGGSIGLGFAIPIDTARKIAQELIRTGHYQHADLGVNAAS
                     VTDGKSDGARVQNVQLGSAAAEGGILENDVIIKVGNRIVGSADGLIVAVREHAIGERV
                     PISLVRDGRPLLVTVTLKSD"
     gene            1282..1686
                     /gene="tatB"
                     /locus_tag="VHH53_07945"
     CDS             1282..1686
                     /gene="tatB"
                     /locus_tag="VHH53_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX2290112.1"
                     /translation="MFNIGWGEFLVLVVGGLFILGPERLPSAAAWLGRTIRQVREYAT
                     GAREQLRSELGPEFDELRQPLEDLRGLRGFNPRGFTPRSAITRHLLDDSDGEPFGDVI
                     KPNGYASPTGRGIPIPRPLQPGERAPFDPDAT"
     gene            complement(1711..2856)
                     /locus_tag="VHH53_07950"
     CDS             complement(1711..2856)
                     /locus_tag="VHH53_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX2290113.1"
                     /translation="MASTQTAPSVDAVRTALDGVHDPEINRPITELGMVKDVTVAGDG
                     TVGVAVWLTVAGCPMRDTITSRITTAVSALPGVRDVQVELDVMSDAQRTSLRRQLRGT
                     AEEPRIPFAEPGSLTRVYCVASGKGGVGKSSVTVNLAAAMADRGLSVGVVDADIYGHS
                     VPGMLGTTARPTQVEKMIMPPTAHGVKVISIGMFTKDNTPVVWRGPMLHRALQQFLAD
                     VFWGDLDVLLLDLPPGTGDIAISVAQLVPNAEILVVTTPQRAAAEVAQRAGAIALQTR
                     QRLIGVVENMSWLQMPDGSRNHLFGSGGGQVVADSLTRSLGSEVPLLGQVPLDPQLRE
                     TGDAGTPLVLADPASPAAAVLRGIADRLATRSRGLAGRLLSVSPHSR"
     gene            complement(2964..3512)
                     /locus_tag="VHH53_07955"
     CDS             complement(2964..3512)
                     /locus_tag="VHH53_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX2290114.1"
                     /translation="MAESHVQYSARRLDLPRGPRRLTPRMDPDAFARFSERIARFLGT
                     GRFLAVQTALVVVWITINLVAVSLRWDPYPFILLNLAFSTQAAYAAPLILLAQNRQDD
                     RDRVALQEDRARAERTKADTEYLARELAALRLAVGEVVTRDFLRSELVRLTEHKELGP
                     ADSTAPDRTGPERSGTRRGGRG"
BASE COUNT          552 a         1155 c         1237 g          576 t
ORIGIN      
        1 gatcggttgg tgaggacgac ttctggccgc ggggcgctgg acgtaacccg tggcgggacc
       61 catatgcggc ggcggtgctg ggggcacccg cggttgccga gcaccaccgg gatccgggcg
      121 acccgtcgcg gccgggagcc cggctgagcc tgcaggaagc gctgttcggt cgtcagctga
      181 gcaaccgcgc gctagccctg ctgtgcgcag tggtgctgtt gatcggggga gtcggcggat
      241 tcgtgggtcg actcaccgcc gacggcggca acctgttgac cgatccaggt gccacgctgg
      301 cgcaagttga gccggcgact gaccggtcgg cgggctcggt ggctgcagtg gccagccggg
      361 tgttgcccgc ggtggtgtcc ctggagttcc gggtcggcga ccagggaggt ttcggttccg
      421 gcgtggtcat cgacggtcag ggctacctgc tgaccaacaa ccacgtggtg gtccccgccg
      481 cgaccacctc gcaggcaacc ttggaagcgg tgttcagcga cggcagccgg gtgccggccc
      541 ggatcgtggg tcgggatccg aagacggatc tcgctgtggt caaagtggag gttgccaacc
      601 ctactgtggc gcagatgggc cggtcctcga ccttgaaacc gggggattcg gtcatcgcta
      661 tcggctcgcc gctgggcctc gccggcacgg tgaccagtgg catcgtcagt gcggtggatc
      721 ggccggtgcg tctggaggga gatggcagtc acggtaacgc ggtgatcgac gcgatccaga
      781 ccgacgccgc gatcaaccct ggcaactccg gcggggcgct ggtgaacacc gcaggagcgg
      841 tggtgggcat caacaccgcc atccgtaccc tgggtgcccg cgagggtggg ggctccatcg
      901 ggctcggctt tgccatcccc atcgacacgg ccaggaagat cgctcaggaa ctgatccgta
      961 ccgggcatta ccagcatgcc gatctggggg tcaacgccgc gtcagtgacc gacggcaagt
     1021 ccgacggagc ccgggtccag aacgtgcagc tgggcagtgc cgccgccgaa ggtggcattc
     1081 tggaaaacga tgtgatcatc aaggtcggca accgtatcgt cggcagtgca gatgggctta
     1141 tcgtcgcggt gcgagagcat gctattggcg aacgggtgcc gatttccttg gttcgtgatg
     1201 ggcggccgct gctggtgact gtcacgctga agtcggattg atcacggctg gctcgagcag
     1261 gctaggcgga ggtgcgcgcg gatgttcaat atcggttggg gggagttcct ggtcctcgtt
     1321 gtcggtggat tgttcatcct gggtcccgaa cgcctgccct cggccgcggc gtggctgggc
     1381 cgcaccattc gccaggtgcg cgagtacgcc accggggccc gtgagcagct tcgttccgag
     1441 ctaggtccgg agttcgacga gctccgccag ccactggagg acctgcgagg gctgcgtggc
     1501 ttcaacccgc gcggtttcac tccacgtagc gcgatcactc gccacctgct cgacgattcg
     1561 gacggcgagc ccttcggtga cgtgatcaaa cctaacggct acgccagtcc gaccggtcgg
     1621 ggcatcccga tccctcggcc gctacagcca ggtgagcgcg caccgtttga cccggacgcg
     1681 acctgagcgc gcacatcccg aggcgggcag tcaccgggaa tggggactta ccgagagcag
     1741 tcgcccggcc aggccgcgtg atcgggtggc gagccggtcg gcgatcccgc gcagcacggc
     1801 cgcggccggc gatgcgggat cggcgagtac cagtggggta ccggcgtcac cggtctcgcg
     1861 cagctgcggg tccagcggca cctgacccag cagcgggacc tcgctgccca gcgagcgggt
     1921 cagcgagtcg gcaaccacct ggcctccacc ggaaccgaag aggtggttgc gcgagccgtc
     1981 cggcatctgc agccacgaca tgttctccac cacaccgatc aggcgctgac gagtctgcag
     2041 cgcgatcgct ccggcccgct gcgccacctc cgccgcggcc cgctgcgggg tggtgaccac
     2101 gaggatctcg gcattgggca cgagttgcgc taccgagatg gcgatatcgc cggtgcccgg
     2161 cggcaagtca agcaacagta cgtctaggtc gccccagaac acgtcggcga ggaactgctg
     2221 cagcgcgcgg tgcagcatcg gcccccgcca caccacgggg gtgttgtcct tggtgaacat
     2281 gccgatggag atgaccttca caccgtgcgc tgttggcggc atgatcatct tttccacctg
     2341 ggtgggccgg gcggtcgtgc cgagcatgcc cggtaccgag tgcccgtaga tgtctgcgtc
     2401 gaccactccc accgataggc cgcggtcggc catcgcggcc gccaggttga ccgtcaccga
     2461 ggacttgccg accccgccct tgccggaggc gacgcaatac acccgagtca gtgatcccgg
     2521 ctcggcgaac ggaatccgcg gctcctcggc ggtgccgcgc agctggcggc gtagcgaggt
     2581 gcgctgagcg tcgctcatca cgtcgagctc cacctgtacg tcacgcacac ccggcagggc
     2641 ggataccgca gtggtgatcc gggaggtgat ggtgtcacgc atcgggcagc cggcgacggt
     2701 cagccacacc gctaccccca ccgtgccatc gccggccacg gtcacgtcct tgaccatgcc
     2761 cagctcggtg atcggccggt tgatttcggg gtcgtgcacc ccgtccagcg cggttcgaac
     2821 cgcgtcgacc gacggggcgg tctgggtgga ggccacgggt agcaccgacc ttctcagttg
     2881 ggggcaggca acttccatgc taggcgcatc gaatcgggcg tcacgatgtc ggcgctcacc
     2941 ttgactgagt gccggcgctg cggtcagccc cgcccaccgc gccgggtgcc ggaccgttcc
     3001 ggcccggtcc gatccggtgc agtggaatcc gccggtccca gctctttgtg ctcggtcagc
     3061 cgcacgagct cggatcgcag gaaatcccgg gtgaccacct cgccaacggc caaccgtagc
     3121 gcagcgagtt ccctggccag gtattcggta tcggctttgg tgcgctcggc tcgggcccgg
     3181 tcctcctgca gtgcgacccg gtctcggtcg tcctggcggt tctgcgccag caggatcagc
     3241 ggcgccgcgt acgccgcctg cgtggagaac gcgagattga gcaggatgaa cgggtaagga
     3301 tcccaacgta gcgacaccgc caccagattg atggtgatcc acaccaccac taaggccgtc
     3361 tgcaccgcca ggaaccgccc ggttcccagg aatcgggcga tccgctcaga gaagcgagcg
     3421 aaggcatccg ggtccattct gggcgtgagg cgccgcggcc cgcgcggcag gtccagccgg
     3481 cgtgccgaat actgcacatg tgactcagcc atgtgtcacc
//
LOCUS       DATADN010000615         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1437887, whole genome shotgun sequence.
ACCESSION   DATADN010000615 DATADN010000000
VERSION     DATADN010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437887"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1461)
                     /locus_tag="VHH53_07960"
     CDS             complement(205..1461)
                     /locus_tag="VHH53_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEX2290115.1"
                     /translation="MVSAARRCGVSRRAFLAGGLGTGAAAMGAGALASCASSSDAGGQ
                     LPEARYVPFEGTHQTGITAIPVPARSLMTAFSVQTSDRAGLRRLFTELTEEIRGLMAG
                     RPIEQRDPTYPPLDSGILGTRPPADDLSVVVSVGSTLFDDRFGLAERKPIELIEMPFL
                     ANDRLDPARSHGDLLLTLEARHPDTIQFALRQLMRRARSDLVLRWMIEGYTRGSVNAA
                     SGGTPRNLLGFKDGTANLPQDDDAVMDRYVWVGAGDPEPGWAAGGAYHVARTIRMFVE
                     FWDRTPLREQEALIGRQKASGAVLGQAAEHDPPAYAEDPDGLVVPLTAHIRLANPRTP
                     ATDENLILRRGFSFSRGFDGSGQLDQGLAFVCYQRSLRKGFLTVQERLSGEPLEEYIM
                     AVGGGFFFALPGVPSPDRFLGDALLA"
     gene            complement(1465..>2239)
                     /locus_tag="VHH53_07965"
     CDS             complement(1465..>2239)
                     /locus_tag="VHH53_07965"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HEX2290116.1"
                     /translation="VEPAIAAYKTYVAGQIDETVTRTKVLTDAVRAGDLAAAQRAYAP
                     SRVPWERIEPIAGLVEEIDGKVDARVDDFAGVDDPTFTGWHRLEYLLFAQNTTEGAAP
                     FADQLDADLRTLKTEFATLEIPPVEAAVGASELIEEVSLGKITGEEDRYAKTDLWDFN
                     GNLEGSKAVVEALTPALQVANPALLADINRGFAELDATLAPLRRGDGWVLYCLQDDEY
                     PSPRCPAVTVSPELVNTLKAQLAGLSEKTSQIAGALRLG"
BASE COUNT          353 a          760 c          777 g          349 t
ORIGIN      
        1 cggatccgca gccgcgatct gcttcgaaga ctttctcacg agacactggg agtggacgcg
       61 tccgggaatg tctatgcgta ccgcaccacg ccggcagcga ccagcgcaga gcggggaaat
      121 ggcgtgagcc gcaacatcaa acatccaacg ccacaacgcc gcgggcggtg ctgacagcca
      181 gcagtcccgc gcatggtgtt acactcaggc gagcagggcg tcgccgagga accggtcggg
      241 gctcggtacc ccgggaaggg cgaagaagaa cccgccgccc accgccatga tgtactcctc
      301 caacggttcc ccggacagcc gctcctggac ggtgaggaaa cccttgcgca ggctgcgctg
      361 gtagcagacg aacgccaggc cctggtcgag ctggccgcta ccgtcgaagc cgcggctgaa
      421 tgagaagccc cggcgcagga tgaggttttc gtcggtggcc ggggtgcgcg ggttggccag
      481 ccggatgtgc gcggtcaacg ggacgaccag cccgtcggga tcctcggcgt aggccggtgg
      541 atcgtgctca gcggcctgcc ctaggactgc gccgctggcc ttctgtcgtc cgatcagcgc
      601 ctcctgctcg cgcagtggcg tgcggtccca gaattcgacg aacatccgga tggtgcgcgc
      661 cacgtggtag gcgccaccgg ctgcccaacc aggctccggg tcaccggcgc cgacccagac
      721 gtaacggtcc atcacggcgt cgtcgtcctg cgggagattg gccgtgccgt ccttgaaacc
      781 cagcaggtta cgtggggtgc caccgctggc tgcgttcacc gagccgcgcg tgtagccctc
      841 gatcatccag cgcagcacga gatcgctgcg ggcgcggcgc atcagctggc gcagcgcgaa
      901 ctggatggtg tcggggtggc gggcctcgag ggtgagcagc aggtcgccgt gcgagcgagc
      961 cgggtccagg cgatcgttgg ccaaaaacgg catctcgatg agctcgatgg gcttgcgctc
     1021 cgccagcccg aaccggtcgt cgaacagcgt ggagccgacg ctgacgacga cggatagatc
     1081 gtcagccggt gggcgcgtcc caagaatgcc ggagtccagt ggtgggtacg tcggatcacg
     1141 ctgctcgatc ggccgccccg ccatcaggcc gcggatctcc tcggtgagct cagtgaacag
     1201 ccggcgcagg cccgcccggt cgctggtctg cacggagaac gcggtcatca ggctgcgcgc
     1261 gggtactggg atcgcagtga tcccggtctg gtgggttccc tcgaacggca cgtaccgcgc
     1321 ctctggcagc tggccgccgg cgtcgctgga gctggcgcag ctggccagtg cccccgcgcc
     1381 catggcggcg gcacctgtcc ccaggccgcc ggccaggaac gcccgccggg agactccgca
     1441 ccgccgcgct gcgctgacca tcgactagcc cagcctgagt gcgccggcga tctgcgaggt
     1501 cttctccgac aggccggcga gctgcgcttt gagggtattc accaactcgg gggacaccgt
     1561 caccgcgggg caacgcgggg aggggtactc gtcgtcctgc aggcagtaga gcacccaccc
     1621 gtcacctctg cgcagcggcg caagcgtggc atccagctcg gcaaatcccc tgttgatgtc
     1681 tgcgagcagc gccgggttgg ccacctgcag cgccggtgtc agcgcctcca ccaccgcttt
     1741 ggacccttcg aggttgccat tgaagtccca gaggtcggtc ttggcgtagc ggtcctcctc
     1801 gccggtgatc ttgccaaggg agacttcctc gatcagctcg gaggcgccga cggcggcctc
     1861 gaccggcggg atctccaggg tggcgaactc ggttttgagg gtgcgcagat cggcgtcgag
     1921 ctgatcggcg aacggcgcgg caccctccgt ggtgttctgc gcgaacagca ggtactccag
     1981 ccgatgccac ccggtgaacg tggggtcgtc gacgcccgcg aaatcgtcga cccgggcgtc
     2041 gaccttgccg tcgatctcct ccacgagccc ggcgatcggc tcgattcgct cccacggtac
     2101 ccgcgacggg gcatacgccc gctgtgcggc ggcaaggtca ccggcgcgga cggcgtcggt
     2161 caacaccttc gtcctcgtca ccgtctcgtc gatctgacca gcgacatagg tcttgtaggc
     2221 ggcgatggcc ggctcgact
//
LOCUS       DATADN010000616         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_216061, whole genome shotgun sequence.
ACCESSION   DATADN010000616 DATADN010000000
VERSION     DATADN010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216061"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VHH53_07970"
     CDS             complement(<1..1375)
                     /locus_tag="VHH53_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX2290117.1"
                     /translation="MSRFVNTLLAHSAPDSPTRSDRGITTGEPAQPRRLTWTQVHDKA
                     LRAAGTLTGSAAPAVQHGDAVAVLAADPALIAPAVQGVWLAGGSVTMLHQPTHRADLA
                     AWAADTVRVLGMIGAKLVLLGPPFDQLAPVLDQRGISYLMLADLDDPAAGPLPAPVAV
                     GEDDTALLQLTSGSSADPKAVRITHCNLYANMTAMAQAGALDPASDVMVSWLPLFHDM
                     GMVGFLTVPMALGLELVKATPSDFMSRPLLWAELISRHRGTITAAPNFAYALLARRLA
                     GADEHFDLSSLRLALNGAEPIEPAAVRAFTAAGAQFGLSPGCMVCAYGMAEATVGVSF
                     APLEIGMQVDTVDADALEGRRRAVPADPSDQHVRAFPRLGPPLPGLEAQVVADDDRVL
                     AEREVGRIRLRGESVTREYLTVEGARPAQDADGWLDTGDEGYLADGDVVICGRRKDVI
                     FMGGRNVY"
     gene            complement(1437..>2009)
                     /locus_tag="VHH53_07975"
     CDS             complement(1437..>2009)
                     /locus_tag="VHH53_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2290118.1"
                     /translation="GDLSGGERRRLQLTRLLMAEPNVLLLDEPTNDLDIDTLQQLEDL
                     LDGWPGSLVVVSHDRYLVERVCDTVVALFGDGRITQLPGGIEEYLQRWDAAAGIGAVV
                     HTGAAQTYPAPPDASTGAAQQRTARKELARLERQLERLADQEAELQRRIAQQAANPSA
                     FRELDGELRAVLAAKDDLETRWLEVADAVG"
BASE COUNT          318 a          726 c          677 g          288 t
ORIGIN      
        1 ggtagacgtt gcgcccgccc atgaagatga cgtccttgcg ccgcccgcag atcaccacgt
       61 cgccgtcggc gaggtatccc tcgtctccgg tgtccagcca cccgtcggca tcctgggcgg
      121 gccgcgcccc ctcaacggtc aggtactcgc gggtcaccga ctcgccccgc aatcggatcc
      181 ggccgacctc gcgctccgcc agcacgcgat catcgtctgc gacaacctgc gcctccagcc
      241 cgggcagcgg tggcccgagc cggggaaagg ctcggacatg ctggtcggat ggatcggctg
      301 ggacggcacg ccgccgcccc tccaacgcgt cggcgtccac tgtgtccacc tgcatgccga
      361 tctccagcgg tgcgaaggac accccgacag tggcctcggc catcccgtag gcgcacacca
      421 tgcagcccgg cgacaaaccg aactgcgctc cggcggcggt gaacgcccgc accgcggccg
      481 gctcgatggg ctcggcaccg ttgagtgcca ggcgcaggct ggacaggtca aagtgctcgt
      541 cggcgccggc caaccgccgg gccagcaggg cgtaggcgaa gttcggcgcc gcggtgatcg
      601 tgccgcggtg gcgactgatc agctcggccc acagcagcgg acgggacatg aaatccgacg
      661 gcgtcgcctt caccagctcc aggcccagcg ccatcggcac ggtgaggaac ccgaccatcc
      721 ccatgtcatg gaacagcggc agccaggaaa ccatgacgtc gcttgccgga tcgagcgcac
      781 cggcctgcgc catcgcggtc atgttggcgt agaggttgca gtgggtgatc cggaccgcct
      841 tggggtccgc gctggaacca gaggtgagct gcagtagcgc ggtgtcgtcc tcgccgaccg
      901 ccaccggcgc aggaaggggt ccagcggccg gatcatcgag atcggcgagc atcaggtagc
      961 tgatgccgcg ctggtcgagt accggggcaa gctggtcgaa gggcggcccg agcaggacca
     1021 gcttggcgcc gatcatgccg agcacccgga cggtgtccgc ggcccatgcc gccaggtcgg
     1081 cgcggtgagt gggctgatgc agcatggtca cgctgccgcc ggccagccag acgccctgca
     1141 ccgcgggagc gatcagcgca gggtcagcgg ccagcacagc gaccgcgtcg ccgtgctgga
     1201 cagcgggcgc ggcactgccc gtcagggtcc cagcggcccg caaggccttg tcgtgcacct
     1261 gcgtccacgt gaggcggcgc ggctgcgccg gttcaccggt ggtgataccg cggtctgatc
     1321 gggtgggcga gtccggcgcg ctgtgcgcca gcagagtgtt gacgaacctg ctcacgtctg
     1381 atcaccttac gggcagtggg tgccggcatg cgcgacgtcc cagcaaccag gtgatgtcag
     1441 ccaacggcgt ccgcgacctc cagccagcgg gtctcaagat catctttcgc agcgagcacc
     1501 gcgcgcagtt caccgtccag ctcccggaac gcgctcggat tcgcggcctg ttgggcgatc
     1561 cggcgctgca gctcagcctc ctggtcggcc aaccgctcca gctgccgctc cagccgagcc
     1621 aactccttgc gcgccgtccg ctgttgcgca gcgccggtcg atgcgtccgg cggtgccgga
     1681 tacgtctgcg cggcgccggt gtgcaccaca gccccgatcc ccgcagctgc atcccaacgc
     1741 tgcaggtact cctcgatgcc gccgggcaac tgggtgatcc gtccgtcacc gaacagcgcc
     1801 accacggtgt cacacacccg ctcgacgagg tagcggtcgt gcgagaccac caccagcgag
     1861 ccgggccagc cgtccagcag atcttccagc tgctgcaagg tgtcgatgtc caggtcgttg
     1921 gtgggctcat caagcagcag cacgttcggt tcggccatca acagccgggt caactgcaac
     1981 ctgcgccgct ccccgccgga caagtcgcc
//
LOCUS       DATADN010000617         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_678950, whole genome shotgun sequence.
ACCESSION   DATADN010000617 DATADN010000000
VERSION     DATADN010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678950"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VHH53_07980"
     CDS             complement(<1..710)
                     /locus_tag="VHH53_07980"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX2290119.1"
                     /translation="MDVRDLTSRLPLPSSQRERGPVVAAVKLHGVITPAQSPLGRGVI
                     NLAAMDPVLTRAFNYDRLRAVALSINSPGGSATQSALVADRIRGLADSKGVPVLAFCE
                     DVAASGGYWLACAADEIVAHPTSLVGSIGVVSQGFGLHGLLERFGVERRLYTAGANKA
                     RLDPFLPERQDDVEWLKSMQTELHGMFCDWVCSRRGGLLAGEQDLFTGEVWTGRRALE
                     LGLVDRLGAMRGEIAKRYP"
     gene            complement(738..1496)
                     /locus_tag="VHH53_07985"
                     /pseudo
     CDS             complement(738..1496)
                     /locus_tag="VHH53_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
BASE COUNT          284 a          572 c          525 g          255 t
ORIGIN      
        1 gggtaacgct tggcgatctc tccacgcatc gcgcccagcc tgtcgaccag ccccagctcc
       61 aaggcccgcc ggccggtcca cacttcaccg gtgaacaagt cctgctcacc ggccagcaga
      121 cccccgcggc gcgagcacac ccagtcacag aacatgccat gcaattccgt ctgcatgctc
      181 ttcagccact ccacgtcatc ttgccgctcg ggcaggaacg gatcgagccg ggctttgttc
      241 gccccggcgg tgtacagccg ccgttccacg ccgaagcgct caagcaggcc gtgcaacccg
      301 aagccctggc tgaccacccc gatggagccg accagcgagg tggggtgagc gacgatctcg
      361 tcagcggcgc acgccagcca gtacccaccg gatgcggcaa catcttcaca gaaggccagc
      421 accgggacgc ccttactgtc ggccaggccg cgaatccgat cggcgaccag cgccgactgg
      481 gtggccgaac cacccggtga gttgatcgac agtgccaccg cccgtaaccg gtcgtaattg
      541 aaagcgcggg tcagcaccgg gtccatcgcg gccaggttga tcaccccgcg gcccagcggt
      601 gactgcgccg gggtgatcac gccgtgcagc ttgaccgcgg ccaccaccgg accgcgctct
      661 cgctggctgc ttggtaacgg cagccgcgag gtcagatccc ttacatccat tcggccagcc
      721 tacgtaggac cgcgtggtta agcgctcact ggcggttgag aacgcgacgt gctcggcgtc
      781 ggcggggcgg gggcacgaac gccacggctg aacttgggca cccgcatgct gaccttggta
      841 ccgactcctg gcccggtctc gacgaccagc ccgcaatcgt caccgaaggc cgcgcgcaac
      901 cgatcgtgca cgttgcccag tcccacgtgc gcgccggaga gatgcgagtc cgtcatctca
      961 tcccgcagcc gggccggatc catcccgata ccgtcgtctt ccacgctgat cacgcactct
     1021 gacccggcgt cctcggcgac gaccgacacc gtgccgccgc ccggcttgcc ggccaggccg
     1081 tgccgcacgg cgttctccac cagcggttgc aacgccagga acggcagcac caccggcagc
     1141 acctcgggtg caatctgcag cttgacctgc agtcgttctc cgaaccgggc tttttccagg
     1201 atcaggtagc ggtcgatgtt gttaagttcc tcggcaagcg tggtgaactc gccggcagcc
     1261 cggaaggagt accgggtgaa ttcggcgaac tccatgagaa gctcgcgagc ctcctccggg
     1321 tcggtgcgca cgcacgagga aatcgtggtg agcgcgttgt agatgaagtg cggcgagatc
     1381 tgggagcgca gggcccgcac ctcggcgtgg gccagccggg atcgggactc gtcgagctcg
     1441 gcgagttcga gctggctgga gacgtaccgg gcgacctcgg cggtggcccg catcactccc
     1501 gccgcagcgc cgcatctgcg ggcgttgctg aactgtgaag ccgtcgcgct gaccaacgac
     1561 gaaagtgtga tgttggcttg ggacggcggc gctcaacatc acgccgaatg ggttgccgag
     1621 gcagcgtttc aggtgg
//
LOCUS       DATADN010000618         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1138355, whole genome shotgun sequence.
ACCESSION   DATADN010000618 DATADN010000000
VERSION     DATADN010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138355"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VHH53_07990"
     CDS             complement(<1..854)
                     /locus_tag="VHH53_07990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HEX2290120.1"
                     /translation="MALALYRKYRPATFAEVVGQEHVTEPLRIALSAGRINHAYLFSG
                     PRGCGKTSSARILARSLNCVKGPTSDPCGVCSSCIALAPEGPGSIDVVELDAASHGGV
                     DDARDLRDRAFYAPAESRYRVFIIDEAHMVTTQGFNALLKIVEEPPEHLVFVFATTEP
                     EKVLATIRSRTHHYPLRLLPPSTMRGLLEQVCAAEAVPIESAVYPLVIHAGGGSARDS
                     LSVLDQLLAGAGAEGITYRRAVALLGVTDVALIDDMVAALAAQDGATVFATIDRLVET
                     GHDPRRFAS"
     gene            complement(954..1337)
                     /locus_tag="VHH53_07995"
     CDS             complement(954..1337)
                     /locus_tag="VHH53_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX2290121.1"
                     /translation="MTGVRREVSNFGTQCVDANVVLRLLNAPEHPAVAELWFEFVAAD
                     VEIVAPTLLTYDLANALRQQWRLGRLGAERAGDLLDVAQALPIALQGDPHLHARALEL
                     AAAHNLPAAYNAHYVALAERFRVPL"
     gene            complement(1288..1614)
                     /locus_tag="VHH53_08000"
     CDS             complement(1288..1614)
                     /locus_tag="VHH53_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX2290122.1"
                     /translation="MSGTERRVSATEARVRFGELLGGVTARHDVVFVERAGKEVAVVV
                     SVEDWKAACAGRSDKWARANAMLAELHERMRQDGTLDRLKDVDWAEVIRDGRAQRGQQ
                     LRDAVR"
BASE COUNT          282 a          565 c          567 g          260 t
ORIGIN      
        1 gaggcgaagc ggcgaggatc gtgtcccgtt tcgaccagac ggtcgatggt ggcgaacacg
       61 gtggccccgt cctgggcggc aagggcggcg accatgtcgt cgatcagcgc cacgtcggtg
      121 actccgagca gcgccactgc ccgccggtaa gtgatcccct cagcgcctgc cccggcgagc
      181 agctggtcca gcacggacag cgagtcccgg gcagacccgc cgccggcgtg gatcaccagc
      241 gggtagacgg cggactcgat gggcaccgcc tctgcggcac atacctgttc cagcagcccg
      301 cgcatggtgc tcggcggcag caaccgtaac gggtagtggt gggtgcggga acggatggtc
      361 gccagcacct tctccggctc ggtggtggcg aagacgaaaa cgaggtgctc cggaggttcc
      421 tccacgatct tgagcaaggc gttgaaacct tgcgtggtga ccatgtgggc ctcgtcgatg
      481 atgaacaccc ggtaccggga ctcagccggg gcatagaagg cgcgatcccg caggtcgcgg
      541 gcgtcgtcca cgccgccgtg gctggctgca tcgagctcga cgacgtcgat agatccggga
      601 ccctcggggg ccagcgcgat gcaggacgag cagaccccac acggatccga cgtcgggccc
      661 ttcacgcagt tcagtgagcg ggccaggatg cgtgcgctgg acgttttccc gcaaccccgc
      721 gggccggaga acaggtaggc gtggttgatc cgtcctgccg agagagcgat ccgcagcggt
      781 tcggtgacgt gctcctggcc gaccacctcg gcgaatgtcg cggggcggta cttgcggtag
      841 agggccagtg ccacggcggc gaggttaccg cgccggtagg acagtgctca cggcacgagg
      901 tgcacctccg gcggtccctg ccccatctcc ttggccagcc gccggtcgca ggttcacagc
      961 ggcacccgaa agcgttcggc cagagcgaca tagtgcgcgt tgtacgcggc cggcaggttg
     1021 tgcgcggccg cgagttcaag cgcgcgggcg tgcaagtgag ggtcgccctg cagggcgatc
     1081 ggcaatgctt gggcgacgtc gaggaggtca cccgcccgtt cggcgccaag cctgccgagg
     1141 cgccactgct ggcgaagcgc gttggctagg tcataggtca gcagtgtcgg ggcgacgatc
     1201 tccacgtctg ccgccacgaa ctcaaaccac agctccgcga ccgctgggtg ctctggagca
     1261 ttcagcagtc gcaagacgac gttggcatca acgcactgcg tcccgaagtt gctgacctct
     1321 ctgcgcacgc ccgtcacgga tcacctccgc ccagtcgaca tccttgagcc gatcaagcgt
     1381 gccgtcctgg cgcatccgct catgtagttc ggcgagcatc gcgttggccc gggcccactt
     1441 gtcggaccgg ccggcgcacg cggccttcca gtcctcgaca gacaccacca cggcaacctc
     1501 cttgcccgcc cgctcaacga aaacgacatc gtggcgggcc gtcacgccgc caagcagctc
     1561 gccgaagcgc acccgggcct cggtcgcgct gactctgcgt tccgttccag acatgccaca
     1621 gctccatcca tcgatcaagc catcgatgga tggagctatc acggtggcgt tgac
//
LOCUS       DATADN010000619         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1269369, whole genome shotgun sequence.
ACCESSION   DATADN010000619 DATADN010000000
VERSION     DATADN010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269369"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VHH53_08005"
     CDS             complement(<1..868)
                     /locus_tag="VHH53_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX2290123.1"
                     /translation="MKVMAQMAMVMNLDKCIGCHTCSVTCKQAWTNRPGTEYVWFNNV
                     ETRPGQGYPRTYEDQERWRGGWVRDKRGRLRLRGGGRLNKLLNIFSNPKLPSIDDYYE
                     PWTYDYENLTNAPLGEHMPVATPRSLISGEPMKVEWSANWDDNLGGSPEIVPGDPVLK
                     QVSERVQLELEQTFMFYLPRICEHCLNPSCVASCPSGAMYKRSEDGIVLVDQDRCRGW
                     RMCVSGCPYKKVYFNHKTGKAEKCTLCYPRIEVGLPTVCSETCVGRLRYLGLVFYDMD
                     RVLAAASVEDDKD"
     gene            complement(865..>1520)
                     /locus_tag="VHH53_08010"
     CDS             complement(865..>1520)
                     /locus_tag="VHH53_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX2290124.1"
                     /translation="RRYAPFTVNIEELKPFHTLTGRMHFYLDHDWLEELGEQLPIYRP
                     PLDVHRLFGEPELGAGGAGGVGLTVRYLTPHSKWSIHSEYQDNLFMLSLSRGGPTMWM
                     SPGDAAKIEVRDNDWVEAVNRNGVLVCRAIVSHRMPEGVVFVYHAQERVIDVPLSETT
                     GKRGGIHNSLTRLLIKPSHLAGGYAQMAFAFNYLGPTGNQRDEVTVVRRRSQEVVYQ"
BASE COUNT          246 a          529 c          467 g          278 t
ORIGIN      
        1 ggtccttgtc gtcctcgacc gacgccgccg ccagcacccg gtccatgtcg tagaacacca
       61 atcccagata ccgcagccgg cccacacacg tctccgagca caccgtcggc aacccgacct
      121 cgatgcgcgg atagcacagc gtgcacttct cggccttgcc ggtcttgtgg ttgaagtaca
      181 ccttcttgta cgggcagccc gacacacaca tccgccagcc gcggcagcgg tcctggtcga
      241 ccagcacgat gccgtcctcg gagcgcttgt acatcgcccc cgacgggcac gacgccacac
      301 acgacgggtt gaggcagtgc tcgcagatcc tcggcaggta gaacatgaac gtctgctcga
      361 gctcgagctg gacccgctcg ctgacctgtt tgagcaccgg gtcgcccggc acgatctccg
      421 gtgagccgcc gaggttgtca tcccagttgg ccgaccactc caccttcatc ggctcaccgc
      481 tgatcaggct gcgcggcgtc gcgacgggca tgtgctcacc cagcggtgca ttggtgaggt
      541 tctcgtagtc gtaggtccac ggctcgtagt agtcgtcgat gctgggcagc ttcgggttgg
      601 agaagatgtt gagcagcttg ttcaaccgcc cgccgccgcg cagccgcagc cggccacgct
      661 tgtcgcgtac ccagccgccg cgccagcgtt cctgatcctc gtaggtgcgt ggataccctt
      721 gtccggggcg ggtttcgacg ttgttgaacc acacgtactc ggtgcccggc cggttggtcc
      781 aggcctgttt gcacgtcacc gaacaggtgt ggcacccgat gcacttgtcg aggttcatca
      841 ccatcgccat ctgggccatg accttcactg gtagaccacc tcctgggagc ggcggcgcac
      901 caccgtcacc tcgtcgcgct ggttcccggt cgggccgaga tagttgaacg cgaacgccat
      961 ctgggcgtaa ccgccggcca ggtggctcgg tttgatcagc aaccgggtca gggagttgtg
     1021 gataccgccc cgcttcccgg tcgtctcgct cagcggcacg tcgatcaccc gctcctgggc
     1081 gtggtagacg aacaccacac cctcgggcat ccggtggctg acgatcgccc gacacaccag
     1141 caccccgttg cggttgaccg cctccaccca atcgttgtcg cgcacttcga tcttcgcggc
     1201 gtcgcccgga ctcatccaca tcgtcggccc gccgcgcgac agcgacagca tgaacaggtt
     1261 gtcctgatac tccgagtgga tcgaccactt cgagtgcggg gtcaggtacc ggacggtcag
     1321 accgacgccg cccgcgcccc ccgcgcccag ctccggttcg ccgaacaagc ggtggacgtc
     1381 caacggcggc cgatagatcg gcagttgctc gccgagttcc tcgagccagt cgtggtcgag
     1441 gtagaagtgc atccgcccgg tcagggtgtg gaacggtttg agctcttcga tgttgacggt
     1501 gaacggcgcg tagcggcgcc
//
LOCUS       DATADN010000620         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_679221, whole genome shotgun sequence.
ACCESSION   DATADN010000620 DATADN010000000
VERSION     DATADN010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679221"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..747
                     /locus_tag="VHH53_08015"
     CDS             286..747
                     /locus_tag="VHH53_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2290125.1"
                     /translation="MSRQQHVVRLTPAERAELRALLAAGTTPARTQTHARILLKGDAA
                     TSGPRWSDARIAEAVEVSARTVARVRARFARGGLAAALGRKRPDRVYWRKLDSAQEAR
                     LIAVACTEPPAGQARWSLRLLANRLVELEIVDGISPETVRATLKKTPSSRG"
     gene            762..1430
                     /locus_tag="VHH53_08020"
     CDS             762..1430
                     /locus_tag="VHH53_08020"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX2290126.1"
                     /translation="MPPQADAAFVAKLEDVVRVYARPPDPARPLVCFDEAGKELRREV
                     RPPRPPAPGHPARHDSEYAREGSANLFLWVAPHLGQRQITVTTQRTAIDWAHAMRDLV
                     DAAFPEAARIVLVQDNLNTHDPASLYKAFPPAEAARIWSRLELHFTPTHGSWLNMAEL
                     ELSALARQCLARRLPDQPTLQQEVTAWAEDRNRAAVGIDWRFSVEDARSTLTHLYPVP
                     EVVT"
BASE COUNT          250 a          566 c          506 g          231 t
ORIGIN      
        1 caaacgtgcc aggatcgctc attccggctc aggcgccatc tacccggacc aggacgctcc
       61 tggaggcggc aaagatcgcg aaagcggcaa agaataccgg gtgatgtcac atgggcaact
      121 tccgagtttc gcgttcgggt cgtgaccgcg catgacatct cggtggaccg tacgaggagc
      181 gacgcgcggc cgacgagatc cttcgttcct cagtagtccg tccagcgtga tcagtcctgg
      241 tgcggtaggc tgggtttccg gttcgtcgcc acccaggagg ccagcatgtc ccgccaacag
      301 cacgtggttc gtctcacgcc cgccgagcgc gccgaactgc gcgccttgct cgccgccggc
      361 accaccccag cccgcaccca gacccatgcc cgcatcctct tgaagggcga tgccgcgacc
      421 agtggccccc ggtggagcga tgcgcgcatc gccgaggcgg tcgaggtcag tgcccgcacg
      481 gtggcccgcg tccgcgcccg cttcgcccgc ggtgggctgg cggcggcgct ggggcgcaag
      541 cgccccgatc gggtgtactg gcgcaagctg gatagcgccc aggaagcgcg gctcatcgcg
      601 gtggcctgca ccgagccgcc ggccggccag gcccgctgga gcctgcggct gctggccaac
      661 cgactggtcg aactggagat cgttgatggc atcagccccg agacggtgcg cgccacgctc
      721 aaaaaaacgc cctcaagccg tggctgaggc agcgctgggt catcccgccc caggccgatg
      781 ccgccttcgt ggccaagctg gaggatgtgg tgcgcgtcta cgcccggccc cccgacccgg
      841 cgcgtcccct ggtctgcttc gacgaggccg gcaaagagct gcgccgcgag gtgcggcccc
      901 cgcggcctcc ggcgccgggc catccggcgc gccacgacag cgagtacgcg cgggaaggca
      961 gcgccaacct ctttctctgg gtggcgccgc acctggggca gcgccagatc accgtgacga
     1021 cccaacgcac ggccatcgac tgggcgcacg ccatgcgcga cctggtcgac gcggcattcc
     1081 ccgaggccgc gcgcatcgtg ctggtgcaag acaacctcaa cacccacgat cccgcctcgc
     1141 tctacaaagc gttccccccg gccgaggcgg cgcgcatctg gagccggctg gaactgcact
     1201 tcaccccgac ccacggctcg tggctgaata tggctgaact cgagctgtcg gcgttggcgc
     1261 ggcagtgctt ggctcgccgt ctccccgatc aacccaccct gcagcaggag gtgaccgcct
     1321 gggccgagga ccgcaaccgc gcggccgtgg gcatcgactg gcggttctcg gtcgaggacg
     1381 ctcggtccac cttgacccat ctctaccccg ttcccgaagt tgtcacatga cgctggacgg
     1441 accactgagc gcgagcgaag aatctcgttc gttttgaccg tctgcgaacg acgaaacgag
     1501 atccttcgtt ccccaggatg acatggctcc tgaccgccgc cctagcgaac gcg
//
LOCUS       DATADN010000621         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_818589, whole genome shotgun sequence.
ACCESSION   DATADN010000621 DATADN010000000
VERSION     DATADN010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818589"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..82)
                     /locus_tag="VHH53_08025"
     CDS             complement(<1..82)
                     /locus_tag="VHH53_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX2290127.1"
                     /translation="MPLTPADVHNVAFSKPPIGKRGYNEDE"
     gene            complement(148..435)
                     /locus_tag="VHH53_08030"
     CDS             complement(148..435)
                     /locus_tag="VHH53_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX2290128.1"
                     /translation="MDVVRLVVYYLLFFFWLLLTARIVVELVRAFARSWQPVGGVAIA
                     LESVYTMTDPPVRALRRVVKPIRIGGVGLDLSIMVLLLVVFILMRVIDPRT"
     gene            complement(507..1241)
                     /gene="sepF"
                     /locus_tag="VHH53_08035"
     CDS             complement(507..1241)
                     /gene="sepF"
                     /locus_tag="VHH53_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEX2290129.1"
                     /translation="MSALYRLKAYFGMVPAEEFDDYYPDYHPDYRSHSRSGYDPGYDA
                     DYDIAASHLSRERDHAAKRPGTSRPNRADSGGYDDDVTTQPGRPSVPPRTHPSRSPSW
                     AAEAPVHGALAIDTTPTVAAPAAPPSPRHPLAGHSLASDPFADPFANDQAYPLSRIVT
                     LHPHNYNEARPIGERYREGNPVIMNLTEMEDRDAKRLVDFAAGLAFALRGSIDKVTNK
                     VFLLSPPNVDVSAEDKRRIAEGRLFG"
     gene            complement(1314..>1744)
                     /locus_tag="VHH53_08040"
     CDS             complement(1314..>1744)
                     /locus_tag="VHH53_08040"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2290130.1"
                     /translation="WARQVQSVDSARLVDALDRASRSAQDVGERDGPLGVLVQASLDS
                     NPVRGGCPLPELEALADRVAAASGLRLEGVMGIAPLDVAAEMAFAELAAAAQRLRRAH
                     PGAVELSAGMSGDLEQAIARGSTCVRVGTALLGDRPLTSH"
BASE COUNT          316 a          573 c          577 g          278 t
ORIGIN      
        1 cctcgtcctc gttgtagccc cgcttgccga tcggcggctt actgaacgcg acgttgtgaa
       61 cgtcggcggg ggtcaacggc atcggatcac ctcacgcact cctcgactat gcacacgctg
      121 gtgtgtcggc cccaagcccg gcggccgcta cgttcttgga tcgatcactc gcatcagtat
      181 gaagaccacg agcagcagaa ccataatgga caggtccagg cccacgcctc caatgcggat
      241 gggtttaacc acccgacgga gcgcccggac cggcggatca gtcatggtat agaccgactc
      301 aagcgcgatc gccacccctc cgaccggctg ccagctacga gcgaaggcgc gaacaagttc
      361 aaccacgata cgcgctgtga gcagcaacca gaagaagaag agcaggtagt acaccaccaa
      421 ccgaaccacg tccacggcga cactctgcca tcggtggcaa gacggggctc aggcgggggt
      481 gaatcgggct cctggactgc gcacagttag ccgaagaggc ggccttcggc gatgcggcgc
      541 ttatcctcag ccgaaacgtc gacgttgggc ggcgacagca ggaacacctt gttggtgacc
      601 ttgtcgatcg agccacgcag cgcaaaggcc aacccggcag cgaagtccac gagacgcttg
      661 gcgtcccggt cctccatctc ggtcaggttc atgatcactg gattgccctc gcggtagcgt
      721 tccccgatgg ggcgggcttc gttgtagttg tgcgggtgca aggtgacgat gcgggacagc
      781 gggtatgcct gatcgttggc gaacgggtcg gcgaacgggt cactggccaa ggaatggccg
      841 gccagtgggt gcctgggcga tggcggcgcg gccggtgcgg cgaccgtggg ggtcgtgtcg
      901 atggccagcg cgccgtgcac cggagcctcg gcagcccacg acggggaccg gctgggatgc
      961 gtcctggggg gtaccgaagg tcgccccggt tgggtcgtga cgtcatcgtc gtaaccgccg
     1021 gaatccgcgc ggttgggccg gctggtgcca ggtcgcttgg cagcgtggtc acgttctctg
     1081 gagaggtggg atgcagctat gtcgtagtcg gcgtcgtagc cggggtcata cccggagcga
     1141 gagtgcgagc gataatcggg gtgatagtcg gggtaatagt catcgaactc ctcagcgggg
     1201 accatgccga agtacgcctt gagcctgtac agcgcgctca tcgcctcgcc ttcctgtccc
     1261 gaccctcgac gcaccgcccg tcgaacccgg tccaggggac gagtcgcaca cggttagtgc
     1321 gaggttagcg gcctatcacc cagtaacgcg gttccgacac gcacgcaggt cgaaccccgc
     1381 gcaatggcct gctcgaggtc cccgctcatt cccgctgaca gttccactgc gccgggatgc
     1441 gcgcggcgta gtcgctgggc ggcggcggcg agctcggcga aggccatctc ggcagcgaca
     1501 tccagcggcg cgatccccat cacaccctcc aaccgcaatc cagaagcggc tgccacccgg
     1561 tctgccagcg cctcgagctc gggcagcggg cagccgccac gcaccggatt gctgtccaag
     1621 ctggcctgca cgagcacacc gagaggaccg tcgcgttccc cgacgtcctg tgcggaccgg
     1681 gaagcccgat ccaacgcatc gaccagtcgt gccgaatcca ccgactgcac ctgcctcgcc
     1741 cacc
//
LOCUS       DATADN010000622         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1138630, whole genome shotgun sequence.
ACCESSION   DATADN010000622 DATADN010000000
VERSION     DATADN010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138630"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..407
                     /locus_tag="VHH53_08045"
     CDS             237..407
                     /locus_tag="VHH53_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290131.1"
                     /translation="MELFASITQPASAKGVAGSDPQATPGSTGVTYRVQVARAAVRHP
                     IEQLPLAETHIA"
     gene            complement(772..>1793)
                     /locus_tag="VHH53_08050"
     CDS             complement(772..>1793)
                     /locus_tag="VHH53_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2290132.1"
                     /translation="ARIDRGEFGTRTLPPTLLTMDPPDHTRLRRLLAPAFTARAIDAL
                     HPRITAVVKSALADLLTAHEVDVIADIGYPIPLAVMCELLDVDIEGAQLLRTETPKLV
                     GILAFDPDEEAILAAEEASASLALHLLPILGDRRHRPGEDLASQLLRLGDDGSGNGAL
                     GLDEVLATCILLLTAGHETTANVIGNGVFTLMQHLDQLHLLVRDPALIKPAIEEILRF
                     ECPVKIVGRTSLIDRQLGGHTIRAGQRVLVLLGAANRDPAQFRDPECFDITRTPAPHL
                     AFGAGPHFCLGAALSRAEAQETLRQLLPLLTEWRLAPGAPAWRVSDTFRALDKLHLVS
                     PATQS"
BASE COUNT          308 a          557 c          615 g          313 t
ORIGIN      
        1 gaacaggacc cgcgcggcaa ttccgtaccg gcgccgaggc ccctccaagg tctcgatctg
       61 gacccgttgc agctcctcgt gcgtgagtcg cggcggctgc ccaggtacgg ccgcgatcgt
      121 gctcccctcg tcagccatca caatccctcc catattcccc ggccctcacc gcagatccgc
      181 tcggtctcaa cgttgggctg cagagcaggc taatcggttt gttgcaggag agcgcggtgg
      241 agctcttcgc gtcgatcact cagcctgcgt cggccaaggg tgtcgccgga tctgatccac
      301 aagcaacacc gggctcaacc ggagtgacct accgggttca agtcgcccgt gcagcggtac
      361 gccaccccat cgagcaacta ccgctggccg aaacccacat cgcctgagag ctccgctgcg
      421 taagcccttc gagagctact ggcgcgctcg gcgcggggag taccgagtcc agtacgtcat
      481 cgatggcacc actcacaccg ttcaggttct ggacgtcagc caccggtggg atgcctaccg
      541 ttgatcgcga agcttacggt ccgtctcgcc gcctgaagga gacggagccg agtaccgcgt
      601 acaacgtgat cgaatcgcct tgagtatgcg agtcgtcgag tgggcttcca acagactgcc
      661 caatcattgt ggttccacca ggttacagtg cgcgatatca aagggcactc ggcaagccac
      721 agcgcatctc cgcgtctgat cggtcaagcg gtccggcgtc gactttcggc gtcaggattg
      781 agtcgcgggg ctgacgaggt gcagcttatc gagtgcgcgg aaagtgtcgg aaactcgcca
      841 ggcgggcgct ccaggtgcga gtcgccactc ggtcagtagc ggcagcagct gccgcagggt
      901 ctcctgagcc tccgcgcgag acagggcagc tccgaggcag aaatgcggtc ccgcaccgaa
      961 agcaaggtgc ggggcgggag ttcgggtgat gtcgaagcac tcggggtcgc ggaactgcgc
     1021 cggatcccgg ttcgctgcgc caagcagaac gagcacgcgc tggccggcgc ggatggtgtg
     1081 cccgccgagt tgccggtcga tgaggctggt ccggccgacg atcttgacgg gacactcgaa
     1141 cctgagtatc tcctcgatcg ctggcttgat cagcgcggga tcacgcacga gcaggtggag
     1201 ttggtcgagg tgttgcatca gggtgaagac gccattgccg atcacgttgg cggtggtctc
     1261 gtgcccggcg gtgagcagca ggatgcaggt ggctagcact tcgtcgagtc cgagtgctcc
     1321 attgccgctg ccgtcgtcgc cgaggcgcag cagctggctg gccaggtcct cgccgggccg
     1381 gtgacggcgg tcgccgagga tgggcagaag atgcagtgcg aggctggcgc tggcctcctc
     1441 ggccgcgagg atcgcctcct catcagggtc gaatgcgagt attcccacca gcttgggggt
     1501 ctcggtacgt aacagctgcg cgccctcaat gtcgacgtca agcagctcgc acatcacagc
     1561 gagcgggatc gggtagccga tgtcggcgat gacgtcgacc tcatgtgctg tgaggaggtc
     1621 ggcaagggcg gatttgacca ccgcggtgat ccggggatgc agcgcgtcga tggcccgcgc
     1681 ggtaaatgcc ggtgcgagca gccgccgcag ccgggtgtgg tcgggcgggt ccatcgtcaa
     1741 cagggtcggg ggcagcgttc gggttccgaa ttcgccgcga tcgatccgtg cgg
//
LOCUS       DATADN010000623         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_385830, whole genome shotgun sequence.
ACCESSION   DATADN010000623 DATADN010000000
VERSION     DATADN010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385830"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..964)
                     /locus_tag="VHH53_08055"
     CDS             complement(74..964)
                     /locus_tag="VHH53_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2290133.1"
                     /translation="MTVVEKASPAAAPPAVPAPPRPKRRRKGSTYLVGGILVLVCWAM
                     LSPVLWTAMTVTKPTDVAFLDPPVFFYEPTLTAFVDLWQTTLFYQYLINTLIVALIST
                     VAALVIGLPAAYALSRFPGWISTVLLIAALVFRALPRFSVVLPMYDISRALGIYDTTF
                     AVAAALVAINQPFSIWLLRNFFAEIPKELDEAAMIDGCSRFGILRRVMIPLMGPGILT
                     AGIFIFLFAFQEYLTANILTDVSARTVPVFIATQLGQTLPMLQQAGAAAMLLTLPVIG
                     IAFIAQKYLVAGLASGAVKG"
     gene            complement(961..>1518)
                     /locus_tag="VHH53_08060"
     CDS             complement(961..>1518)
                     /locus_tag="VHH53_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2290134.1"
                     /translation="SYVVPNVVGATMFSWLFDANFGGVVNHLVTVVTGQDVLWFTAPW
                     PNRVIVALNTIWFMLPFAMLVIIAGLQGVPDELLEAARIDGASAVRTHWSVIIPSIRG
                     VLGFVAIISIMDVLRTYDQLVALSPQAVQLGNESIMLYIFNVAFQDGGQQLGLGSAVN
                     VLLILLIVLMLFPFIRGIAKEARQA"
BASE COUNT          285 a          487 c          541 g          205 t
ORIGIN      
        1 gctgccgggc atgaactgct cggcagcagc acaagggggt aggtgtccac cgtcatcggt
       61 caggctcgag agctcagcct ttcacggctc cagacgccag gcccgcgacg aggtacttct
      121 gggcgatgaa cgcgatcccg atgaccggca gggtgagcag catggcggcc gccccggcct
      181 gctgcagcat cggcagcgtc tggccgagct gcgtggcgat gaacaccggc acggtgcggg
      241 cggagacgtc ggtgaggatg ttcgcggtga ggtactcctg gaacgcgaac aggaagatga
      301 agatcccggc ggtcaggatg cccggcccca tcagggggat catcacgcgg cgcaggatgc
      361 cgaaccggct gcagccgtcg atcatcgcgg cctcgtcgag ctccttgggg atctcggcga
      421 agaagttgcg cagcagccag atgctgaacg gctggttgat cgcgacgagc gcggccgcca
      481 ccgcgaaggt ggtgtcgtag atcccgagcg cgcgggagat gtcgtacatc ggcagcacca
      541 ccgagaaccg cggcagcgcc cggaacacca gcgcggcgat cagcagcacg gtgctgatcc
      601 agccggggaa ccgcgacagc gcgtacgccg caggcagccc gatcaccagc gccgcgaccg
      661 tgctgatcag cgccacgatc agggtgttga tcaggtactg gtagaagagc gtggtctgcc
      721 agaggtcgac gaacgccgtc agcgtcggct cgtagaagaa caccggcggg tcgaggaacg
      781 ccacgtcggt gggcttcgtg acggtcatgg ccgtccacag caccgggctg agcatcgccc
      841 agcagacgag gacgaggatg cccccgacca ggtaggtcga ccccttgcgg cgccgcttcg
      901 ggcgcggcgg cgcgggcact gccgggggcg cggcggcggg cgaggccttc tccacgacgg
      961 tcatgcctgc ctggcctcct tcgcgatgcc ccggatgaag gggaagagca tgagcacgat
     1021 cagcaggatc agcagcacgt tcaccgcgct gccgagcccg agctgctggc cgccgtcctg
     1081 gaaggccacg ttgaagatgt agagcatgat cgactcgttg ccgagctgga ccgcctgcgg
     1141 cgagagcgcg acgagctggt cgtacgtccg caggacgtcc atgatcgaga tgatcgcgac
     1201 gaagcccagc acaccgcgga tgctcgggat gatcacgctc cagtgcgtgc ggaccgcgga
     1261 ggcaccgtcg atgcgcgccg cctccagcag ctcgtcgggc acgccctgca gccccgcgat
     1321 gatcacgagc atcgcgaacg gcagcatgaa ccagatcgtg ttgagcgcga cgatcacccg
     1381 gttcggccac ggggcggtga accagagcac gtcctggccg gtgacgaccg tgaccaggtg
     1441 gttgaccacg cccccgaagt tcgcgtcgaa cagccaggag aacatcgtgg cgcccacgac
     1501 gttcggcacg acgtagga
//
LOCUS       DATADN010000624         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1269681, whole genome shotgun sequence.
ACCESSION   DATADN010000624 DATADN010000000
VERSION     DATADN010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269681"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VHH53_08065"
     CDS             <1..790
                     /locus_tag="VHH53_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX2290135.1"
                     /translation="VPAGLEGYHPGADVEVQLLRGLAEIDRTIVAIGSHDLVLDLAAS
                     ALRGTDPGVTLASSNVGSLGGLVALRDGLCHLAGSHLLDPETGQYTVSYLPRVFGDAL
                     AVADVAVVRLVHRDQGLIVAAGNPLGLAGIEDLSRPGLRYVNRQRGAGTRMLLDQELR
                     RRGMAPGDVAGYRREEHTHLAVAAAVAGGRADAGLGILAAARPFGLDFVPVKQEPYDL
                     VVTRAAYDSEVLAPLWTLLADEKFRASVEALGGYSSAETGRRVC"
     gene            827..1534
                     /locus_tag="VHH53_08070"
     CDS             827..1534
                     /locus_tag="VHH53_08070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2290136.1"
                     /translation="MDNALQNVTFPSNGSTAHGYLSLPESGSGPGVVVIQEWWGLTNH
                     IADVANRLAAEGFVALAPDLFGGATTHDADEATELMQKLPVQRAARDLGGAVDYLLGH
                     DAVTSSTIGAVGFCMGGGFVLVLAAQQGDKIGAAVPFYGVLGEPYPSFEHLRAPLLGH
                     FGDQDEMQSTDQVRELAATIERQSGRKPDFRFYPAGHAFFNDQNLLGTYDPVQAAKAW
                     DATVSFLRQQLATPQRG"
BASE COUNT          222 a          531 c          544 g          270 t
ORIGIN      
        1 cgtgccggcc gggctggagg gttaccaccc tggcgcggat gtcgaggtgc agttgctgcg
       61 cgggctggct gagatcgacc gcaccatcgt ggcgatcggt tcgcatgatc tggtgctcga
      121 cttggccgcc tcagccctac gtggcaccga ccctggcgtg acgctcgcct cgtccaacgt
      181 cggctcgctg ggcgggctgg tggcgctgcg cgacgggctg tgccacctcg ccggctcaca
      241 cctgctcgat cccgagaccg gccagtacac cgtgtcctac ttgccgcggg tcttcggcga
      301 tgccctcgcg gtcgccgatg tcgcagtggt ccgcctggtg catcgtgacc agggcctgat
      361 cgtcgccgct ggtaatcccc tggggctcgc cggcatcgag gacctcagcc gtccgggact
      421 gcgctatgtc aaccggcagc gtggcgccgg cacccggatg ctgctggacc aggagctgcg
      481 ccgtcgcggc atggcaccag gcgatgtcgc cggttaccgg cgcgaggagc acacccatct
      541 cgctgtcgca gccgcggtgg caggcgggcg agcggatgcg gggctcggca tcctcgcggc
      601 cgcccggccg ttcggactgg acttcgtgcc cgtcaagcag gagccctacg acctcgtcgt
      661 cacccgggcc gcctacgact ccgaggtgct ggccccgctg tggacgctgc tggcggacga
      721 gaagttccgc gcctcggtgg aggctctcgg cgggtactcc agcgccgaga ccggccgccg
      781 ggtgtgctga acgcggcaac cggatgactg tggtgggatc acggccatgg acaacgcgct
      841 gcagaacgtc acgtttccgt ccaatggttc caccgcacac ggctacctgt cgctgccgga
      901 gtcgggcagc ggtccgggtg tcgtggtgat ccaagaatgg tgggggctca ccaaccacat
      961 cgccgacgtg gcgaaccggt tggctgccga gggattcgtc gctctggcac ccgacctctt
     1021 cggcggtgcc accactcacg acgccgacga ggcgaccgag ctcatgcaga agctacccgt
     1081 gcagcgggcg gcccgtgacc tgggcggcgc ggtggactac ctactgggcc acgacgcagt
     1141 caccagctcc acgatcggtg cggtcggttt ctgcatgggt ggcggcttcg tgctggtgct
     1201 cgctgcccag cagggcgaca agatcggcgc cgcggtgccg ttttacgggg tgctcggcga
     1261 gccgtacccg agctttgagc atctccgcgc gccgctgttg ggtcacttcg gcgatcagga
     1321 cgagatgcag agcaccgatc aggtgcgtga gctcgcggct accatcgaac ggcagtccgg
     1381 ccgaaagccg gatttccgct tctatccggc ggggcacgcc ttcttcaacg atcagaacct
     1441 gctcggcacc tacgatccgg tccaggccgc caaggcttgg gacgccacgg tgagttttct
     1501 gcgccagcag ttagcgactc cccagcgcgg ctaacctcag atgtcgatgc tgaggtcggc
     1561 tcggatc
//
LOCUS       DATADN010000625         1579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_532621, whole genome shotgun sequence.
ACCESSION   DATADN010000625 DATADN010000000
VERSION     DATADN010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532621"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VHH53_08075"
     CDS             <1..256
                     /locus_tag="VHH53_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290137.1"
                     /translation="DDRWGERPLATVVIAKDAEVSAGELRDHLGASVPAWQLPERWSF
                     LDDIPKTSVGKFDKKLIRTHYANGDLNVVHIHRYGDISGS"
     gene            complement(275..1561)
                     /gene="murA"
                     /locus_tag="VHH53_08080"
     CDS             complement(275..1561)
                     /gene="murA"
                     /locus_tag="VHH53_08080"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX2290138.1"
                     /translation="MSEHFRVRGGARLTGEVQVVGAKNSVLKLMAAALLAEGTTTLTN
                     CPEILDVPLMADVLRGLGCDVDVTGSEVAISTPAEPKHEADYGSVSKLRASVCVLGPL
                     VGRCRRARVALPGGDAIGSRPLDMHQNGLRKLGARSEIEHGCVVAEAEGLHGAQIWLD
                     FPSVGATENILMAAVLAEGTTVLDNAAREPEIVDLCVMLQQMGARISGAGTSTLTIEG
                     VDVLRPTEHRVIGDRIVGATWAFAAAMTRGDVTVRGVDPHHLDLVLEKLRLAGAHVEQ
                     APTDHAGLDGFRVVMPDRPRSVDFVTLPYPGFATDLQPMAIALSAVSEGTSMITENVF
                     EARFRFVEEMIRLGADARTDGHHAVVRGVERLSSAPVWASDIRAGAGLVLAGMCAEGT
                     TDVWDVFHIDRGYPRFVENLAALGADIQRVTAPPER"
BASE COUNT          268 a          555 c          507 g          249 t
ORIGIN      
        1 ggacgacagg tggggggagc ggccgctggc cacggtggtc atcgccaaag acgcagaggt
       61 cagcgctggg gagctccggg atcacctggg tgcctcggtg cctgcctggc agcttcccga
      121 gcgctggtcg ttccttgatg acatacccaa gacctcggtg ggcaagttcg acaaaaagct
      181 catccgcacc cactacgcca acggcgacct caacgtcgtc catatacacc gatatgggga
      241 catatcgggg tcttgaccac cccgctttgg gtggtcagcg ctctgggggt gcggtgacgc
      301 gctggatgtc ggcacctaga gctgccaggt tctccacgaa gcgagggtag ccgcggtcga
      361 tgtggaagac gtcccagacg tcagtggtgc cctccgcgca catcccggcg agcaccagtc
      421 ccgcgccggc ccggatgtcc gacgcccata ccggggcgct ggagagccgc tcgacgcccc
      481 ggacaaccgc gtgatgtcca tcggtgcgcg catcggcgcc cagccggatc atctcctcga
      541 cgaaccggaa ccgggcctcg aacacattct cagtgatcat cgacgtgcct tcagagacgg
      601 ctgacagggc gatcgccatc ggctgcaggt cggtggcaaa acccgggtaa ggcaacgtca
      661 cgaagtccac cgacctgggc cggtcgggca tcaccacccg gaaaccgtcg agtccagcgt
      721 gatcagttgg tgcttgctcg acgtgggcgc cggccagtcg cagcttctcc agcaccaggt
      781 ccaggtggtg cgggtcgaca ccccgcacgg tgacgtcgcc gcgcgtcatc gcggcagcga
      841 aggcccaggt ggcgccgacg atgcggtccc cgatcacccg gtgttcggtc ggccgcaaca
      901 cgtccacccc ctcgatggtg agcgtggagg tgccggcccc ggagatccgg gcacccatct
      961 gctgcagcat cacgcagaga tccacgatct ccggttcgcg ggccgcgttg tccaggactg
     1021 tggtgccttc cgcgagcacc gcggccatca agatgttctc ggtggcgccc acgctgggga
     1081 agtccagcca gatctgcgcc ccatgcagcc cttcagcctc ggccaccacg cagccgtgct
     1141 cgatctcgct gcgcgcaccc agcttgcgaa gcccgttctg gtgcatgtcc agcgggcggg
     1201 agccgatggc gtcgccgccg ggcagcgcga ccctggctcg ccggcagcgg cctactagcg
     1261 gcccgagcac gcacaccgag gcgcgcaact tgctgaccga gccgtaatcg gcctcatgct
     1321 tgggctcagc cggcgtgctg atggcgactt ctgaaccggt gacatcgacg tcgcaaccaa
     1381 ggccccgcag cacgtccgcc atcagcggta cgtccaggat ttccgggcaa ttcgtcagcg
     1441 tcgtcgtgcc ctcggccagc aacgccgcgg ccatcagctt cagcacactg ttcttcgcgc
     1501 cgacgacctg cacttcgccg gtcaacctgg caccaccacg aaccctgaag tgctcgctca
     1561 cgggagtgca cgctagcgg
//
LOCUS       DATADN010000626         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1139168, whole genome shotgun sequence.
ACCESSION   DATADN010000626 DATADN010000000
VERSION     DATADN010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139168"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..894)
                     /gene="trpC"
                     /locus_tag="VHH53_08085"
                     /pseudo
     CDS             complement(85..894)
                     /gene="trpC"
                     /locus_tag="VHH53_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
     gene            complement(964..1557)
                     /locus_tag="VHH53_08090"
     CDS             complement(964..1557)
                     /locus_tag="VHH53_08090"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HEX2290139.1"
                     /translation="MPEAAASRRVLTATVVLLIAAAVTLGAAAVLGWAWVDFQVPKRG
                     IVPIRVDGSEVLPALTPLAVLALAAVAAVLATGGWTRRLFGGLLLEAAVPPILGVLQA
                     PDEGWLTAAAMSAAGWSAGSVHAETTTLLPAGPSFAAGGAILLIAAGVTLVLRGHRMP
                     RLGRRYQAPSSRRSGPEDGLWARMDAGEDPTAPGHPR"
     gene            complement(1550..>2407)
                     /locus_tag="VHH53_08095"
     CDS             complement(1550..>2407)
                     /locus_tag="VHH53_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX2290140.1"
                     /translation="RRRSPEQHHAAVETAKEAIRAGEAFQVVVSQRFEMATDADPLDI
                     YRVLRTTNPSPYMYLLALDEFSIVGCSPESLVTVRDGKVTTHPIAGTRPRSADPDEDS
                     WLEKDLMADEKERAEHLMLVDLGRNDVGRVCRPGSVHVTDFFRVERYSHVMHLVSTVT
                     GELAAGHTAFDAVTACFPAGTLSGAPKPRAMELIEELEPTRRGVYGGVVGYLDFAGDA
                     DTAIAIRTALVRNGVAYVQAGGGIVADSDPEAEDTECLNKAGAVLAAIAAAGTLRVAV
                     GEPAGSLRA"
BASE COUNT          361 a          895 c          815 g          336 t
ORIGIN      
        1 cctcaggcac gaaccgcccg ccatagtgcc cgaagtgccc gcgggcgtca ggctgggcgg
       61 taccggactg gacgctgacc acgctcaacg caccggcttc gggcaggccg ggtgcgaacc
      121 ggcggtgacc agctggacga ccgcggcctt ggggtcgccg ctggtcacca gtccctcacc
      181 gacaagcacc gcgtccgctc ccgcaccggc gtaggccagc agatccgccg gtccgcgcac
      241 tccggactcg gcgacgcgca agacgtcacc cggcagccca ggcgcgatct tgctgaacac
      301 cgcgggattc acctccagcg tgtgcaaatc gcgagcgttg actccgatca gagttgcccc
      361 ggcctccaac gcgcggtcag ccttctcggc ggtgtgcacc tcgaccagcg cggccatacc
      421 cagctactcg acccggtcga ccagcgccac cagcgcgttc tgctccagtg cggcgacgat
      481 gagcagcacc acgtccgcac cgtgcgcgcg tgcctcgtgc acctggtagg ggctgacgat
      541 gaagtccttg cgcagcactg gcacgtccac cacagcacgc accgcgtcca ggtcagccag
      601 cgatccgccg aaccgccggc cctcggtcaa cacgctgatc atgcgggcgc cggcctgggc
      661 gtagtcggcg gcgagcgcgg ccggatccgg gatatcggcc agggttcctc gggacgggct
      721 gcagcgcttg acctcggcga tgacgccgat tccggggccg cgtagcgccg ccagagcatc
      781 ccgaggaggt gtcacccggg tggcgcgccg cttgatctcg gtgaacccaa cggcggcttc
      841 gcggtccgcg aggtccgctc tgaccccttc gaggatcgcc tccaagacgc tcaagcctct
      901 ccccttccgc cccccagtgc cgagccggac ctcgatgcta gctccggccg gtccggtatc
      961 ccctcaccgg gggtgtcccg gcgcggtcgg gtcctcgccg gcgtccatcc gcgcccacag
     1021 cccgtcctca ggccccgacc ggcgcgacga gggcgcctgg taacgacgac ccagtcgcgg
     1081 catccggtgg cctcgcaaca ccaaagtgac gccggcggcg atgagcagga tcgctccacc
     1141 tgccgcgaag ctgggtcccg cgggcagcag tgtggtggtc tcagcgtgga ctgacccggc
     1201 ggaccacccg gccgccgaca tggcggccgc ggtcagccag ccctcatcag gtgcctgcag
     1261 caccccgagg atcggcggga ccgccgcctc cagcagcagc ccgccgaaca gccggcgggt
     1321 ccacccgccg gtggccagta ctgcggccac tgcggccagc gcgagtacgg ccagtggcgt
     1381 cagtgcgggc agcacctcgg agccgtccac tcggatcggg acgatcccgc gcttgggcac
     1441 ctggaagtcg acccaggccc agccgagcac cgccgcggca ccgagcgtga ccgcggcggc
     1501 gatcagcagc acgacggtgg ccgtgagcac cctgcgcgac gcggcagcct caggcacgca
     1561 gcgacccggc gggctcaccg actgccaccc gcaaggtgcc cgcagcggcg atggcagcga
     1621 gcaccgcgcc tgccttgttc aggcactcgg tgtcctcggc ctccgggtcg gagtcggcga
     1681 cgatgcctcc accggcttgc acgtaggcca ccccgttgcg gaccagcgcg gtacggatgg
     1741 cgatcgcggt gtcggcatcc ccggcgaagt cgaggtagcc gaccactccc ccgtacacgc
     1801 cccggcgggt tggctccagc tcctcgatca gttccatcgc ccggggcttg ggagctccgg
     1861 acagcgtccc agcgggaaag catgcggtca ccgcatcgaa tgcggtgtgc ccagccgcca
     1921 gctcgccggt caccgtcgac accaagtgca tcacgtggct gtagcgctcg acccggaaga
     1981 agtcggtgac gtgcaccgag ccgggccggc acacccggcc gacgtcgttg cgaccgaggt
     2041 cgacgagcat gaggtgctca gcacgctcct tctcgtcggc catcaggtcc ttctccagcc
     2101 aggagtcctc gtccgggtcg gcgctgcggg ggcgggtgcc ggcgatcggg tgtgtggtga
     2161 ccttcccgtc ccgcacggtc accagtgact ccgggctgca gccgacgatg ctgaactcgt
     2221 cgagagcgag caagtacatg tacgggcttg gattggtggt gcgcagcacc cggtagatgt
     2281 ccagcgggtc ggcgtcggtt gccatctcga agcgttggga tacgactacc tgaaaagcct
     2341 cgccggcccg gatcgcctcc ttggccgttt cgaccgcggc gtgatgctgc tcggggctgc
     2401 ggcggcg
//
LOCUS       DATADN010000627         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_60723, whole genome shotgun sequence.
ACCESSION   DATADN010000627 DATADN010000000
VERSION     DATADN010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60723"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..477
                     /locus_tag="VHH53_08100"
     CDS             193..477
                     /locus_tag="VHH53_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290141.1"
                     /translation="MEGQKSGARSNRAHDANCSLLRLAAGVLRMRYVHFSRDTSLSPA
                     AADELANTLEGVAEGRPVFDRIDPREAIALAHRLVDDDHPELSGMWPRTR"
     gene            707..1321
                     /locus_tag="VHH53_08105"
     CDS             707..1321
                     /locus_tag="VHH53_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HEX2290142.1"
                     /translation="MTAAVDTEAPVRSAPAGDPALIGVPTFIVGAIALGLVLTGYVPA
                     GAVGASIAIILPATGLGQLVAALWAAALGQNAVAGVFGVFTGFWLSYAALVLGLIHGW
                     FGISGDAAVATQGLFLISWLVTIVLLTLATLRLPAAFTLLFTLIDIALALVLYGTVSA
                     STAATTAGGYVVFAFTAVGVYMFAGALFAATGGRPLPLGRPVLN"
     gene            1479..>1695
                     /locus_tag="VHH53_08110"
     CDS             1479..>1695
                     /locus_tag="VHH53_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290143.1"
                     /translation="MPVSDQAFRRRRECSYTQLLTSQATGGAAAPWAARPVVLFQEDD
                     QTKGKAMRFEIVRLDDADGTPVDTTVVD"
BASE COUNT          261 a          569 c          570 g          295 t
ORIGIN      
        1 agcagcaagg accagcccca acacccccgg ctttgctggg ctcaggcgcg tcgggattcg
       61 caccgctctg accggataat ctactggtgc tcgggacctg gctacggcaa ttgagcacga
      121 ccagtcctcc agctatccga cccgccaaga gacggaaccc tcgggagact tgaagaggaa
      181 aagggagtcg gcgtggaagg ccaaaagtca ggtgcccgat ccaatcgggc ccacgatgcc
      241 aactgctccc tcctgcggct ggccgcggga gtgctgcgga tgcgctacgt gcatttcagc
      301 cgggacacca gcctgtcccc ggccgcggca gacgagctgg caaacacctt ggagggtgtc
      361 gccgagggcc gtcccgtctt tgaccggatc gatccaaggg aggcgatcgc cttggcgcat
      421 cgcctggttg acgacgacca tccggagctg tcaggaatgt ggcccaggac ccggtaagcg
      481 gtcggagatt tcaccgggcg cgtaatggcc ggtgggccgt ggccgggatc tcggattgat
      541 ccggtggctg ttttccttca cggaaatcat cccggcgagg tgaccgggct cggagcggaa
      601 agcgggtggc gccatcgcgt ctcctgcttt cactgcacct ggccgatcgg ccgccgcccg
      661 gcccgaccgc actgagaatc actcgcagct caacgaggag tacacgatga ccgctgccgt
      721 tgacaccgaa gcccccgtcc gatccgcacc cgcgggcgat cccgccctga tcggcgtgcc
      781 caccttcatc gtcggggcga tcgcgctcgg cctggtgctg accgggtacg tcccggccgg
      841 tgccgtcggt gcctcgatcg cgatcatctt gcccgcgacc ggcctgggcc agctcgtcgc
      901 cgcgctgtgg gcagcggcac tgggccagaa cgctgtcgcc ggcgtgttcg gtgtgttcac
      961 cgggttctgg ctcagctacg cggcacttgt gctcggcctg attcacggct ggttcggcat
     1021 ctccggcgac gcggcggtcg ccacgcaggg cctgttcctg atctcctggc tcgtcacgat
     1081 cgtgctgctg acgctggcca ccctgcggct gcccgccgcg ttcacactgc tgttcacatt
     1141 gatcgatatc gcgctcgcgc tcgtgctgta cggcacagtg agcgcctcca cggctgccac
     1201 caccgcgggc gggtacgtgg tgttcgcgtt caccgcggtc ggggtgtaca tgttcgccgg
     1261 cgcactgttc gccgcgacgg gcggcaggcc gctgccgctg ggccgtccgg tgctgaactg
     1321 aggtgacacg aggtgggccg acacaggttg cccagaagca gctcggtgcg ggcagtgagg
     1381 gacgcgaggt cgtcgttcct tgcctctgtt gtcctggcgc tcacgctgcg aacacaactg
     1441 ctcccggccg gtcccggcgg ccggtcccag gagaacactt gccggtcagc gatcaggcgt
     1501 ttcgccggcg tagggagtgt tcgtacacgc agttgttgac cagccaggca acagggggcg
     1561 ccgctgcccc gtgggccgcc cgcccagtag ttctctttca ggaggatgac cagacgaagg
     1621 gaaaagcgat gcgcttcgaa atcgtgcgac tcgacgacgc cgacggcacc cccgtggaca
     1681 ccaccgtcgt ggacg
//
LOCUS       DATADN010000628         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_988867, whole genome shotgun sequence.
ACCESSION   DATADN010000628 DATADN010000000
VERSION     DATADN010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988867"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VHH53_08115"
     CDS             <1..1468
                     /locus_tag="VHH53_08115"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEX2290144.1"
                     /translation="LDPAGLLGVDTATGPDEQNLEAVYFDTADLRLVRAGVTLRRREG
                     GSDEGWHLKFPAGKDSRDELRLPLGRATRRPPAELVALTRVYTRGGALAPVAELNTRR
                     RRWVLADSRGRSLAELVDDHVRARTMGEQATAVSWREVEVELGENGQRALLDRIERQL
                     LKAGAQRAGSASKLGRLLADRMPPSVATPTPASARSAGDVVLAYLWEQVEQLRRYDPL
                     VRQDAPDAVHQMRVAARRMRSALQAFGRILDRNQTRELTEELKWVAGELGGARDAEVM
                     AERFAAILAEFPDDLKLGPVDAALTRTFERRQADAREVAVAALDSERYLALHDRIDAL
                     LAAPPVTARARRKAKRELPKNVRRAYRRVESRMADADRQPAGQQRDLALHETRKAAKR
                     LRYATEAVEPTVGKPAARLRKRLKAVQEVLGEHQDTAVSRPVLRQLAAQAHLEGGNGF
                     TYGLMYATEATRADQVENNLPRAWKQMRKSKNIAWLEK"
     gene            1635..2090
                     /locus_tag="VHH53_08120"
     CDS             1635..2090
                     /locus_tag="VHH53_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX2290145.1"
                     /translation="MALTLTSSAFADNEFIPVEHTCDGDNVSPPLEWSQVPDGTAELV
                     LLFEDPDGPGGTFVQWVLFGLDPASGGLQEGKVPAGAIGGKNDYGRTDWAGPCPPIGK
                     PHRFIFTLLALSEPSGLAEGASPRNHLPQALSGKVLAQAQLTGRYARRR"
     gene            complement(2096..2794)
                     /locus_tag="VHH53_08125"
     CDS             complement(2096..2794)
                     /locus_tag="VHH53_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX2290146.1"
                     /translation="MPEDWQRALAVVAHPDDLEYGGAAAIARWTADGRTVTYLLATRG
                     EAGIDSLPPTECGPLREAEQRAAAAEVGVATVEFLDHPDGLVEYGVPLRRDIAAAIRR
                     HRPELVIITNHRETWPGGGLNMADHRNGGLAAIDAVRDAANRWLFPELGLQPWNGVRW
                     VAAVASPLAGHAVEVSTTFDRGVASLAAHRAYLQALGGAMADPATFLRDMAQVDAKRL
                     PGAHLATAFELIPV"
BASE COUNT          463 a         1015 c         1039 g          412 t
ORIGIN      
        1 gctcgatccg gcggggttgc tcggggtcga caccgcgacc ggaccggatg aacagaacct
       61 ggaggcggtg tacttcgaca ccgccgattt gcgcctggtg cgggccgggg tgacgttgcg
      121 acgccgggag ggcgggtccg acgagggatg gcatctgaag tttccggcgg gcaaggacag
      181 ccgtgatgag ctgcggctac cgttgggacg tgccacccgc cgcccaccgg ccgagctggt
      241 cgcactcacc cgggtgtaca cccgcggtgg cgcgctggcc ccggttgctg agctgaacac
      301 ccgccggcgc cgctgggtgc tcgccgattc ccgcgggcgg tcgctggcag aactggttga
      361 tgaccacgtg cgtgcccgaa ccatgggtga gcaggccacg gccgtgtcat ggcgtgaggt
      421 ggaggtcgag cttggtgaga acggccagcg ggcgctgctg gaccggatcg agcgccagct
      481 gctcaaggcc ggcgcgcagc gtgcgggctc ggcgtcgaag ctgggtcggc tgctggccga
      541 ccggatgccg cccagcgtcg ccacgccaac ccccgcttct gcacgttctg ctggggacgt
      601 ggtgctggcg tacttgtggg agcaggtcga gcagctgcgc cggtacgacc ccctggtgcg
      661 ccaggacgcc ccggacgcgg tccaccagat gcgcgtcgct gcccgccgga tgcgcagcgc
      721 actgcaggca ttcggtcgga tcctcgaccg caaccagact cgtgagctga ccgaggagct
      781 gaagtgggtg gccggggagc tgggtggggc cagggacgcg gaggtgatgg ccgagcggtt
      841 cgcggcgatc ctcgcggagt tccccgatga cctcaagctc ggaccggtcg acgccgcgct
      901 tactcgcacc tttgagcgca ggcaggccga cgcccgcgag gtcgccgtgg ccgccctgga
      961 cagcgagcgc tacctcgccc tgcacgaccg gatcgacgcg ttgctggccg ccccgccggt
     1021 caccgcccgc gccaggcgca aagccaagcg cgagctaccg aaaaacgtcc ggcgggccta
     1081 ccgccgcgtc gaatcccgta tggccgacgc ggatcgtcag ccagccggcc agcagcgcga
     1141 tctcgcgctg cacgaaaccc gcaaggccgc caaacggctg cgctacgcca ccgaggccgt
     1201 cgagcccacg gtgggtaagc cggccgcgcg gctgcgcaag cgcctcaagg ccgtccagga
     1261 agtgctcggg gagcaccagg acactgctgt gtcacggcca gtgctgcgcc agctcgccgc
     1321 ccaagcccac ctcgagggcg gaaacggctt cacctacggc ctcatgtacg caactgaggc
     1381 cacccgcgcc gaccaggtgg agaacaacct cccacgagcg tggaagcaga tgcgcaaaag
     1441 caagaacatc gcctggctgg agaagtgagt gcgccgtatg cggccaggag ttaaccactc
     1501 tggcggatga actcgtccac catcggcggc gccatggcac agattgccac aaccagtacc
     1561 gacaattccc ggcactgaag atgacccacg aacccacccg agagcggtgc gcaaggctgg
     1621 tagcgtcgat tgatatggcg ctcacactca ctagctcagc cttcgccgac aacgagttca
     1681 ttcccgtcga gcacacctgt gacggcgaca acgtgtcgcc cccgctggag tggtcgcagg
     1741 tcccggacgg caccgctgag ttggtcctgc tcttcgaaga tcctgatggg ccgggaggca
     1801 cgttcgtgca gtgggtcctc ttcggactcg atcccgccag tggtgggctg caggagggca
     1861 aggtgcccgc cggagccatt ggcggcaaga atgactatgg ccgcacggat tgggccggac
     1921 catgcccccc gatcggcaag ccgcaccgct tcatcttcac cctgctcgcg ctgtcggagc
     1981 ccagcggcct ggccgagggc gccagccctc gcaatcattt accgcaggcg ctgtcgggca
     2041 aggtgctggc ccaagcccaa ctcaccggta ggtacgcccg ccggcgctga gctacctaga
     2101 ccgggatcag ctcgaaggcg gtcgcgaggt gggcgcccgg caaccgcttg gcgtcgacct
     2161 gcgccatgtc gcgcaggaac gtggccgggt cggccatcgc cccgccgagc gcctgtaggt
     2221 aggcacggtg cgcggccaac gacgcgacac cccggtcgaa cgtggtgctc acctccaccg
     2281 cgtgcccagc cagcggcgag gcgaccgctg ccacccagcg gaccccgttc cagggctgca
     2341 gcccgagttc ggggaacagc caacggttgg ccgcgtcgcg gacggcgtcg atggccgcga
     2401 gcccgccgtt gcggtggtcg gccatgttca gccccccacc cggccaggtc tcccggtggt
     2461 tggtgatgat caccagttcc ggccggtgcc ggcggatcgc ggcggcgatg tcgcggcgca
     2521 gcggcacgcc gtactcgacg agcccgtcgg ggtggtcgag gaactcgact gtcgcgacgc
     2581 cgacttccgc cgccgcggca cgttgctcgg cctcccgcag cggcccgcac tcggtcggcg
     2641 gaagggaatc gataccggcc tcaccgcggg tggccagcag gtaggtgacc gtgcggccgt
     2701 cagccgtcca gcgggcgatc gccgcggcgc cgccgtactc caggtcgtcg gggtgggcga
     2761 cgacggccag cgcgcgctgc cagtcttcgg gcatcggact gtagggctcg agcacctgct
     2821 gcacgctctc actccgacgg gggcacggca agggggttgg gaacggtgcc gttgggttcc
     2881 agcgcgtgcc gcagcgcgcg atcgctgtac cgggtcacga ccagccgat
//
LOCUS       DATADN010000629         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_988956, whole genome shotgun sequence.
ACCESSION   DATADN010000629 DATADN010000000
VERSION     DATADN010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988956"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VHH53_08130"
     CDS             <1..1301
                     /locus_tag="VHH53_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290147.1"
                     /translation="RAGTYLFAKLYAVSHLRSDRWYKLGRELLYGRLEDEKPFNTVRR
                     LVQQEDYALRLCRDAGLSSPRPYGVVELTPEREYLLVTEFFDGAVELSEAEVDDAVID
                     GGLQIIHKLWAAGLAHRDIKPANLLVRDQQVLLIDVAFIELRPTPWRQAVDLANMMLC
                     LALRSSPERVYQRALGLFTVEEITEGFAAARGLALPSQLRHMLRAQGRNLHAEFVQLL
                     PTPPQPIPIQRWSMRRVGLLAAMVLLLVLVFNQGLSLDYQEAVETPTRVGNLACTDLE
                     PQWLMAQSVPSASLVPCVRSLPAGWSVAEVTVNDGRSVIPLSHDRAGTGVLVVRLTAA
                     CDTRGATQVRSDQPQVRRYQRIDRLGPRFEATRFDLFPGGCVSAQAAVPAANRAEVTS
                     QLATILAYSTRSALQQALDQRSGGRLRLDPATARSSRQPA"
     gene            2547..>3709
                     /locus_tag="VHH53_08135"
     CDS             2547..>3709
                     /locus_tag="VHH53_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2290148.1"
                     /translation="MLALGLDGVPVGLLRRLINHGVMPNLAAFAGAGSLHQMATTLPA
                     SSSVAWASFVTGCQPGRHGVYGLTDLRPDSYDVSFTSSSELRAPAIWDRAAMSGRRSL
                     VVNVPGTYPARKLRGTMVAGFVAPDLRRACHPPVLADRLTADGYRIDVDARCMPDDPE
                     GFSSDLLGTLAARCRAILALVAEERWDLAVAVVTETDRLHHFFYDRYERSEPQAVELF
                     ERVYRTVDDLVGRLLSSVAEGTRVMLLSGHGFCSVRAHVYLNTWLADHGYLRASSVAA
                     VDRETRAFALDAGRVYLNTTNQFPAGSFAMSDPDYEDLLAKLAQGLGSIVDPDSGEPV
                     IAQVHRRRDIYSGPQKRRGPHLVAVPQPGYELKGMMGRPMLGPSGPFAGTHTAA"
BASE COUNT          544 a         1276 c         1298 g          591 t
ORIGIN      
        1 accgcgccgg gacgtacctg ttcgcgaagc tgtacgccgt ctcgcacctg cggtcggacc
       61 ggtggtacaa gctcggccgg gagctgttgt acggccggct ggaggacgag aagccgttca
      121 ataccgtgcg gcggctggtc cagcaggagg actacgcgct gcggctgtgc cgcgacgccg
      181 gcctatccag ccccagaccc tacggggtcg tggagctcac ccccgagcgg gagtacctgc
      241 tggtcaccga gttcttcgac ggcgcggtgg agctgagcga ggccgaggtc gacgacgccg
      301 tcatcgacgg cggcctacag atcatccaca agctgtgggc ggccgggctt gcccaccgcg
      361 atatcaagcc ggccaacctg ctggtccgcg accagcaggt gctgctgatc gacgtcgcct
      421 tcatcgaact caggcccacc ccctggcgtc aggccgtgga tctggccaac atgatgctgt
      481 gcctggctct gcgttccagc cccgaacgcg tctaccagcg agcccttggc ctgttcaccg
      541 tggaggagat caccgagggg ttcgcggccg cccgcggcct ggccctcccc tcccagctgc
      601 gccacatgct gcgggcccag ggccggaacc tgcacgccga gttcgttcag ctgctgccga
      661 ccccgcccca gccgatcccg atccaacgct ggagcatgcg gcgagtcgga ctcctggccg
      721 ccatggtgct gttgctggtg ctggtgttca accaggggct gtcgctcgac taccaggagg
      781 ccgtcgagac gccaaccagg gtcggcaacc tggcctgcac cgatctcgag ccgcagtggc
      841 tgatggccca gtcggtgccg tcagcgtcac tggtgccatg cgttcgttcc ctgccggccg
      901 gctggtcagt ggcggaggta acggtcaacg acggccggtc ggtaatcccc ctgagccacg
      961 accgcgcggg caccggcgtg ctggttgtcc gactgacggc tgcctgtgac acccgggggg
     1021 ctacccaggt gcgctccgac cagccgcagg tgcgacgcta ccagcggatc gatcgcctgg
     1081 ggccccgctt tgaagccact cgcttcgacc tgttcccggg cggctgcgtc agcgcacagg
     1141 cggccgtgcc ggcggcaaac cgggccgagg tcaccagcca gctggccacg atcctggcct
     1201 actccacccg gtcggccctc cagcaggcgc tggaccagcg ctcgggtggc cgactgcggc
     1261 tcgacccggc gactgctcga tcatcccgac aacccgcgta gttcctttcg acccggctcc
     1321 atccaccacc actgctaccc gggcaaggct gctccgaggt tgctgagcgg gcgacggcgc
     1381 tgatcagctg gtggatctga cggttgtagt tgtcggcgag ctgctacctt ccgatcgcaa
     1441 tcggatgcga ggcgttaggc cgaggcgagc acgctgccgc cacgcaggat gggtggcaac
     1501 ctctttggca acccaggcgc tggtaccagg caccacagga cggtaccgga tggaagcgcc
     1561 gccgcagctg agcagccagg atgggtctgg gcgggtccag ccggagggtt agggaccgac
     1621 tcgtaatcgg aaggtcgtcg gttcgaatcc gacctcgggc tccaaacagc aggtcagagg
     1681 gcgttctaga gatggctgag tgtgcatcgg cgactggcgg tcattccttt gggttggatc
     1741 atcgcgcggt aggcgcgccc gagcgctacg ccgctccgct acgtaggagt ccgtctggtc
     1801 gttagagaga gcccgtcgac gcgcctcctt ggtcggccta ggacgggcgg aatcatggaa
     1861 gacgggacaa ttcagccggc ctgcgggccg ggcgcgcatt cgcgcgcatc tcagaaaacc
     1921 aactctgtca gctttgctcc ttccagacgg cgactgttgg ctggcaccgg cctgggttgg
     1981 cgtcaccacg cggcggcagg actgtgaggg gtcacgcccg agggcggaca ggcttctgat
     2041 caggccagcg atgatgtggg ctaggccggt gccggcagct cgaggacaga tcagccgcag
     2101 agcacacctg gcggcggcca gcgaggactg gggtcactct ccctgccggc gccagcctgc
     2161 cagcacccag accaggcacc acagccagca tcacagcgag gactgacctg acagcggctt
     2221 ttcgctgcag aacggccacc gaggcggggc gagcaggcga tcgtccgctg agtccctgag
     2281 cggcccagga aggtccggcg cggcgtcggg ctgggtcgtc ttcttcggcc agggtacggt
     2341 ttgtgagaaa cggttcgtga gaaactccac ggatttagtg gagaagtacg ctcgatgcag
     2401 gttcgggggt gctcctcgag gtgttgtggt gagaggtggc gccttgagcg ccgcgagcgc
     2461 gcgccgatac agctgtaggg ccatggcgcg cgccggtacg gacaggtggt gaggcgatgg
     2521 gctgggttcg aaggcgcgga gcgggcgtgc tcgccctcgg gctggacggc gttccagtcg
     2581 ggttgctccg gcgcctgatc aaccacggcg tcatgccgaa cctggccgcc ttcgccggtg
     2641 ccggctcgct gcaccagatg gccaccacgc tgccggccag ctcctcggtc gcctgggcga
     2701 gcttcgtgac cggctgccag cccggccggc acggcgtgta cggcctcacc gacctgcggc
     2761 ccgactccta cgacgtgtcg ttcacctcct cgtcggagct gcgcgccccg gccatctggg
     2821 accgggcggc catgagcggc cgccgctcgc tggtggtcaa cgtgccgggc acctatcccg
     2881 cgcgcaagct gcgcggcacg atggtggccg ggttcgtggc gccggacctg cggcgtgcct
     2941 gccacccgcc cgtcctggcc gaccggctga cggcggacgg ctaccgcatc gacgtggacg
     3001 cccggtgcat gcccgacgac cccgagggct tctcgagcga cctgctcggc accctggcgg
     3061 ccaggtgccg ggcgatcctg gcgctggtcg ccgaggagcg ctgggacctg gccgtggccg
     3121 tggtcaccga gaccgaccgg ctccaccact tcttctacga ccgctacgag cgttccgagc
     3181 cgcaggcggt cgagctgttc gagcgcgtgt accgcacggt cgacgacctt gtcggccggc
     3241 tgctgtcgag cgtggcagag ggcacgcgcg tcatgctcct gtccggccac gggttctgct
     3301 ccgtgcgcgc acacgtgtac ctcaacacct ggctggccga ccacggctac ctgcgcgcct
     3361 cctcggtggc ggccgtggac cgcgagacgc gcgcgttcgc gctggacgcg ggccgggtgt
     3421 acctgaacac caccaaccag ttccccgccg ggtcgttcgc gatgtcggac cctgactacg
     3481 aggatttgct ggccaagctc gcccaggggc ttggctcgat cgtcgacccg gactccggtg
     3541 agccggtgat cgcccaggtg caccggcgcc gggacatcta cagcggtccg cagaagcggc
     3601 gcggcccgca cctggtcgcg gtgccgcagc ctggctatga gctcaagggc atgatgggcc
     3661 ggccgatgct cggcccgagc gggccgttcg ccggcaccca caccgccgc
//
LOCUS       DATADN010000630         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_217706, whole genome shotgun sequence.
ACCESSION   DATADN010000630 DATADN010000000
VERSION     DATADN010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217706"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VHH53_08140"
     CDS             <1..479
                     /locus_tag="VHH53_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2290149.1"
                     /translation="AATKQIAADPRLAGVHVVILTTFELDEYVFEALRTGASGFLVKD
                     TEPADLISGVRAVAGGDALLSPGVTRRLIAEFATRAKQPVDTAALSALTDREREVMAL
                     VAQGLSNEDIASTLFVSPATAKTHVSRAMVKLGARDRAQLVVIAYEAGLVRPGWRP"
     gene            complement(554..997)
                     /locus_tag="VHH53_08145"
     CDS             complement(554..997)
                     /locus_tag="VHH53_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2290150.1"
                     /translation="MTLVTWCDFVIASERAEFGFPEVRIGVPTIVGAIRLPRKINWQY
                     AMELLLTGERIDATRAHEIGLAGWVVPHGELMDAARSLADRLVRGAPLAARAIKEVAA
                     RTADLPMTDAIRFGETMRRVVGATEDAAEGRAAVLERRDPVWRGR"
     gene            complement(975..1289)
                     /locus_tag="VHH53_08150"
     CDS             complement(975..1289)
                     /locus_tag="VHH53_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290151.1"
                     /translation="MNDTVRYQLDGHVATITYHRPERLNAIDGATTRTRGWASSPVPA
                     GRSAQGRTYAARATRPVSSGHILGAPDGELVRERLGDLQTGHRGSQRVLPRLRVDAGH
                     LV"
     gene            1447..1980
                     /locus_tag="VHH53_08155"
     CDS             1447..1980
                     /locus_tag="VHH53_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX2290152.1"
                     /translation="MVDGAVPTEPVALADALVEVLRLRGRPALRVDAADFLRPASVRL
                     EHGRTDPDELLHGWLDVAGLRREVLEPSGPAGSGQVLPRLWDSRVDRAYRAQRVELAA
                     NGVLLLAGALLLGRGLPAELTVHLQLSAAALARRLDPQLRWTLPAYRRYDAEHDPAAA
                     DVLVLADDPRRLALMHR"
     gene            2098..>2448
                     /locus_tag="VHH53_08160"
     CDS             2098..>2448
                     /locus_tag="VHH53_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2290153.1"
                     /translation="MSTPQITLTARLSTSALDARRGVVRLHPEVLDALGLRPWDAVRL
                     TGARVSAALAAAGQTAPGIALLDDLTLSNLGLTEGSEVMLTPAQVPAARSITVSGSAL
                     VSHSVSPETLRLALA"
BASE COUNT          354 a          873 c          849 g          372 t
ORIGIN      
        1 tcgcggcaac caagcagatc gctgccgatc cccggttggc gggcgtgcac gtggtcatcc
       61 tgaccacctt cgagctcgac gagtacgtct tcgaggccct gcgcaccggc gccagcggct
      121 tcctggtcaa ggacaccgaa ccggccgacc tgatcagcgg ggtgcgcgcc gtggccggcg
      181 gcgatgcact gctgtccccg ggcgtcaccc ggcggctgat cgcagagttc gccacccgcg
      241 ccaagcagcc ggtggacacc gcggcgctga gcgctctcac cgatcgggaa cgcgaggtga
      301 tggcgctggt cgcccaaggt ctgtccaatg aggatatcgc cagcacgctg tttgtcagcc
      361 cggcaacggc caagactcat gtcagccgcg cgatggtcaa gctcggcgcc cgggaccgcg
      421 cgcagctcgt ggtgatcgct tacgaggcag gcctggtccg tccaggctgg cggccctagc
      481 cccaaggcgc aacaggtagg gcatgagggc ctgacacacc gtgcatgaga gacggacatg
      541 cgcggtgtgg gggttagcgg cctcgccaca ccggatcccg ccgctccagc acagcggccc
      601 ggccctcggc ggcgtcctca gtggcgccta ccacccggcg catggtttcg ccgaaccgga
      661 ttgcatccgt catcggcagg tcagcggtgc gcgccgcgac ctccttgatc gcccgtgcgg
      721 ccagcggcgc accacgcacc agccggtcag ccaacgatcg ggcagcgtcc atcaactccc
      781 cgtgcggcac cacccacccg gccaaaccga tctcgtgggc acgtgtggcg tcgatgcgct
      841 ctcccgtgag cagcagctcc atcgcgtact gccagttgat ctttcggggc agccggatcg
      901 cgccgacgat cgtcggcacc ccgatccgga cctcggggaa gccgaactcc gcccgctcgc
      961 tggcgatcac gaagtcacac caggtgacca gcgtcaaccc gtagccgagg cagaacccgt
     1021 tgactgccgc gatgaccggt ttgaagatct cccagccgct ctcgaacgag ttcgccgtcg
     1081 ggcgctccca gaatgtgccc cgaactcacc ggtcgggtcg ccctcgccgc gtaggtccgc
     1141 ccctgcgcag aacgcccggc cggcaccggt gacgatgccc acccacgcgt cctggtcgtc
     1201 gcgccgtcga tcgcgttgag ccgctccggg cggtggtacg tgatcgtcgc gacatggccg
     1261 tcgagctggt agcgcacggt gtcattcacc ggcgcagtat tacgccagcg ccacctgcca
     1321 cagcacttga gtcgaaacgc aaccgggagg actgtgagcg aggtgtgggt ccaacccatc
     1381 cgtgagcccg cgctgctcga cgagctggcc gaacgggtca cgcggtttcc cggccgtgct
     1441 cgggtcatcg tcgacggcgc tgtgccgacc gagccggtcg cgctggccga cgccctggtc
     1501 gaggtgttgc ggctgcgcgg tcgaccggcg ctgcgggtgg atgcggcgga cttcctccgg
     1561 ccggcctcgg tgcggctgga acacggccgc acggatcccg acgagctgct gcacgggtgg
     1621 ctggacgtag ccgggctacg ccgcgaggtg ctcgagccgt ccggacccgc cggttccggc
     1681 caggtgctac cccggctgtg ggacagccgg gtggaccgtg cctaccgggc gcagcgggtg
     1741 gagctggcgg ccaacggggt cttgctgctg gccggcgcgc tactgctcgg gcggggtctg
     1801 ccggccgagc tcaccgtgca cctgcagcta tccgctgccg cgctggcccg caggctggac
     1861 ccgcagctac gctggacgct gccggcctac cgccgctacg acgccgagca cgacccggcc
     1921 gccgcagacg tgttagtact ggccgatgat ccgcgccggc tcgcgctgat gcaccgctga
     1981 gcgctgccgc cgtttgatcc tgccccaggg cttgtccaca gagatcttgc agtctggatg
     2041 cagactgacg gcgcaacagg ggcgggatgt gacgggagcc tcgtaggctc ggacgtcgtg
     2101 agcacaccgc agatcacctt gaccgcaagg ctcagcacct cggcgctgga cgcgcggcgc
     2161 ggggtggtgc ggctacaccc ggaggtgctc gacgccctgg gcttgcggcc gtgggatgcc
     2221 gtgcggctca ccggcgctcg ggtgagcgct gcgctggctg cagccgggca gaccgcccct
     2281 ggaatcgcgc tgctggacga tctcacgctg tccaacctcg ggctgacgga gggctccgaa
     2341 gtcatgctca caccggcgca ggtgcccgcg gctcgatcga tcaccgtcag cggttcggcg
     2401 ctggtcagcc attcggtgag tcccgagacg ctgcggttgg cgctggcc
//
LOCUS       DATADN010000631         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1439807, whole genome shotgun sequence.
ACCESSION   DATADN010000631 DATADN010000000
VERSION     DATADN010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439807"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1965)
                     /gene="eccCb"
                     /locus_tag="VHH53_08165"
     CDS             complement(<1..>1965)
                     /gene="eccCb"
                     /locus_tag="VHH53_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCb"
                     /protein_id="HEX2290154.1"
                     /translation="AQKPEFIDLFVAIGRLGRSLQMHLLLASQRLEEGRLRGLESHLS
                     YRIGLKTFSAGESRAVLGVPDAYELPPLPGSGYLKFDTMSMERFKAAYVSGPYRVGGA
                     LTMASTGAVTSDRRPRLFVPEYVDLPEVAQPPGQQPVFDAPPGDLAEPSVLDVVVAKL
                     GGQGPPAHEVWLPPLEAPPSLDQLLPPLSAIEDRGLSPVGFFGNGRLAVPVGIVDKPY
                     EQRRDLLWADFSGAAGHAVVTGGPQSGKSTLLRCLLLSLALTHTPEEVQFFCIDFGGG
                     TLASLDGLPHSGGVAGRLEPDTVRRIIAEVTGLLTAREKRFRSLGIDSMADFRARKQR
                     GPSAQTQQLREDLFGDVFLVIDGWHSFRQEFDASDLEVKVVNLATLGLSYGVHIVIGA
                     TRWAEIRPALKDLLGTRFELRLGDPGESDIDRRVAVNVPQGRPGRGLSREKLHFLAAL
                     PRIDAVTSAQDVGLGVADAVARIRGEWPGSHAPRVRLLPERVPYEQLPALQSQRPHLI
                     PIGINEVELAPVYLDFTAEPHFLCFADGESGKTSLLRTIARGIVDSCSPDQARIVMVD
                     YRRTMLGFLDSEHVIGYGMSADVLIPIIADVERSMRKRLPGASVTQQQLRDRSWWSGP
                     ELYVLVDDYDLVATQGGANPLAPLLEFLAQA"
BASE COUNT          352 a          677 c          664 g          272 t
ORIGIN      
        1 ggcctgcgcc aagaactcga gcagcggcgc gagcgggttc gcgccgccct gggtggccac
       61 caggtcgtag tcatcgacca ggacgtacag ctcagggccg gaccaccagg agcggtcccg
      121 gagctgctgc tgggtaaccg acgccccggg caggcgcttg cgcatcgagc gctccacatc
      181 ggcgatgatc ggaatcagca catcggcgga catcccgtac ccgatgacgt gctcggaatc
      241 caggaagccg agcatggtcc ggcggtagtc gaccatgacg atgcgggcct ggtccgggct
      301 acagctgtcc acaatgccgc gggcgatcgt ccgaagcagg ctggtcttgc ctgattcccc
      361 gtcggcaaaa cacaggaagt gcggctcagc ggtgaagtcg agatacaccg gggcgagctc
      421 aacctcgttg atcccgatcg ggatcaggtg cggacgctga gattggagcg cgggcagctg
      481 ctcgtaaggg actcgctcgg gcagcaaccg gacccgcggc gcgtgggagc caggccactc
      541 gccacggatg cgcgccaccg cgtcagcgac cccaaggccg acgtcctgcg cgctggtcac
      601 cgcgtcgatc ctgggcagcg cggcaaggaa atgcagtttc tccctggaca gtccccgacc
      661 gggccggccc tgcggcacgt tgaccgccac ccgccgatcg atatcggact cgcccgggtc
      721 accaagccgc agctcgaagc gagtaccgag caggtccttg agggcgggcc ggatctccgc
      781 ccaccgggtg gcgccgatca cgatgtgcac tccgtaggac aggcccagag tggccagatt
      841 gacgaccttc acctccaggt cgctggcgtc gaactcctgc cggaagctgt gccacccgtc
      901 gatcaccagg aacacgtcac cgaacagatc ttcccgcaac tgctgtgtct gagctgacgg
      961 gccgcgctgc ttgcgggccc ggaaatcggc catcgagtcg attcccagtg agcggaaccg
     1021 cttctccctg gcggtgagca gcccggtcac ctcggcgatg atccgccgca ccgtgtcggg
     1081 ctccagccgc ccggccaccc ccccgctgtg cggcaggcca tccagcgatg ccagcgtgcc
     1141 gcccccgaag tcgatgcaga agaactgcac ttcttcgggg gtatgggtga gcgccagaga
     1201 caacagcagg cagcgcagca gggttgactt gcccgactgc gggccgccgg tgaccacagc
     1261 atggcccgcg gccccggaga agtccgccca gagtaggtca cggcgctgtt cgtagggttt
     1321 gtccacgatc ccgaccggga cggccagccg cccgttgccg aagaacccga ccggggatag
     1381 cccgcgatcc tcgattgctg acagcggagg cagcagctga tccaacgagg gcggcgcctc
     1441 cagcggcggc agccacacct cgtgggcagg gggtccctgc ccgcccaact tggcgaccac
     1501 gacatccagc acgctgggct cggcgagatc tccaggcgga gcgtcgaaca ccggctgctg
     1561 gcccggcggc tgggctacct cgggcagatc cacgtactcg ggcacgaata gccgcggccg
     1621 ccggtctgag gtgacagcgc ccgtgctggc catcgtgagg gcaccgccga cccgataggg
     1681 gcctgagacg taagccgcct tgaagcgctc catggacatg gtgtcgaact tcaggtagcc
     1741 cgatccgggc agcggcggca gctcgtaggc gtcgggaaca ccgagcacgg cccgagactc
     1801 cccagcggag aacgtcttga gaccgatccg gtaggacagg tgggactcca ggccgcgcag
     1861 cctgccctcc tcgagacgct gcgaggccag cagcaggtgc atctgcagcg aacggcccag
     1921 ccggccgatg gccacgaaca ggtcgatgaa ctcaggcttc tgggc
//
LOCUS       DATADN010000632         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_820394, whole genome shotgun sequence.
ACCESSION   DATADN010000632 DATADN010000000
VERSION     DATADN010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820394"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VHH53_08170"
     CDS             complement(<1..1063)
                     /locus_tag="VHH53_08170"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX2290155.1"
                     /translation="MTVSESARLFGRASTVIPGGVNSPVRAFRAVGGTPRFMVRASGP
                     HLWDADGTRYVDLVSSWGPMILGHAHPAVVDAVRAAASRGLSFGTPTTGEIELAEEIV
                     RRVEPVEQVRLVNSGTEATMSAIRLARGATGRSAVVKFAGHYHGHVDALLVAAGSGIA
                     TFGLPSSPGVTGAQAVDTIVLPYNDIDAVRAVFAARGGEIACVIAEAAAGNMGAVPPD
                     PGFNAALRELCHAHGALLVLDEVMTGFRVSAAGWFGLEQVPADLYTFGKVMSGGLPAA
                     AFGGSAELMSHLAPAGPVYQAGTLSGNPVAVAAGLATLRAADREVYARLDAAAAWLAG
                     LLDEALDAQSVPHVVQTAGS"
     gene            complement(1425..1685)
                     /locus_tag="VHH53_08175"
     CDS             complement(1425..1685)
                     /locus_tag="VHH53_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290156.1"
                     /translation="MWVRLRRRVLVTVAEVLVICVLVWLTWWCWHRGVIITEHNGVTL
                     RRIEGRWWAAATGAATLAGILVLDALRQMMSGALRAPASVRE"
BASE COUNT          319 a          628 c          555 g          223 t
ORIGIN      
        1 gcgacccggc ggtctgaacc acatgcggca cgctctgggc gtccaatgcc tcgtcgagca
       61 gcccggccag ccaagcggct gccgcgtcca gccgggcgta gacctcccgg tcggcggctc
      121 gaagcgtggc caaaccggca gccaccgcga ccggattccc ggacaacgtg cccgcctgat
      181 agaccggccc ggccggtgcc agatgcgaca tcaactcggc cgaaccaccg aacgccgccg
      241 ccggcagccc gccggacatc accttgccga aggtgtagag gtcggccggc acctgctcaa
      301 gcccgaacca gccggcggcc gagacccgaa atccggtcat cacctcgtcg agtaccagca
      361 gcgcgccgtg ggcgtggcac agctcgcgga gtgcggcgtt gaaaccgggg tcgggtggta
      421 ccgcgcccat gttgccggcg gctgcctcag cgatgacgca ggcgatctcg ccaccccgcg
      481 cggcgaacac cgcgcgcacc gcgtcgatgt cgttgtaggg cagcacaatc gtgtccaccg
      541 cctgcgcgcc agtgacaccg gggctcgacg gcaacccgaa ggtggcgatg ccggacccgg
      601 cggcgaccag cagcgcatcg acgtgtccgt ggtagtgccc ggcgaacttc accaccgcgg
      661 agcggccggt agcgccgcgg gccagccgga tcgcactcat cgtcgcctcg gtgccggaat
      721 tgaccaggcg gacctgctcg accggctcga cccggcgcac gatctcctcg gcaagctcga
      781 tctccccggt ggtgggggtc ccgaaggaca gccctcgtga cgcggccgcg cgcaccgcgt
      841 ccaccaccgc cgggtgcgca tggcccagga tcatcggccc ccaggacgac accagatcga
      901 cgtagcgagt gccgtcggcg tcccagaggt gaggccccga tgcccggacc atgaagcgcg
      961 gcgtgccacc gaccgcacga aacgcccgca ccggggagtt caccccaccg gggatcacgg
     1021 tgctggcgcg gccgaacagc cgggccgatt cgctgactgt cacgtcgccc cgcggctgcg
     1081 aatcacggtg cacagcgcgg ccaccacggc atcgccgggg gtgacggtgg gatgcacaaa
     1141 cgaatgatac gcggcacctt cgtgctgaca tggcgccagg tgaaccggag taatatgcgt
     1201 gtcatcgcat accaacttta gcgttcaggc gggagttcgg aatgacgcaa tccacccaat
     1261 acagcaaggg cactctactt acttgtgagc atgaggcgtg taactgccgg gttcggatcg
     1321 aggaagaatg ccactgcccg tccggcggcc agccgtacac gtgtgcctgt ggagcgcccc
     1381 tggtcgaggc gagcgaggtc gccagctcct agcggtaggg cagctcattc tcgaacggat
     1441 gccggcgcac gaagtgcacc ggacatcatc tgccgcagcg cgtcgagcac caggataccg
     1501 gccagcgtgg ccgcgccggt ggccgcggcc caccagcgcc cctcaatccg gcgcaacgtc
     1561 acaccgttgt gctccgtgat gatcacgcca cgatgccagc accaccaggt caaccagacg
     1621 agcacgcaga taacgagcac ctcagccaca gtgaccagga cacggcgacg caaccgcacc
     1681 cacaactccc ctcctaagcc ccttcacctc ccgatatggc gacat
//
LOCUS       DATADN010000633         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1440365, whole genome shotgun sequence.
ACCESSION   DATADN010000633 DATADN010000000
VERSION     DATADN010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440365"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="VHH53_08180"
     CDS             <1..1400
                     /locus_tag="VHH53_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2290157.1"
                     /translation="IRTMADIGSKQPGVENAVQFPGLSINGFVNAPNAGIVFFTLKPF
                     DERHGSEQYGLNIAGALNAKFAGIQDAFVAVFPPPPVNGLGQVGGFKLELEDRAGLGE
                     AALNEATQALLARAWQTPSLAGVFSGYRVNVPQLDVEVDREKVKREGIVLTDLFQTMQ
                     AYLGSVYVNDFNKFGRTYQVKVQADAQFRATADDIAQLKVRNAQGAMVPLGSVVQVKE
                     SHGPDQATRYNGYPAADINGGPAPGYSSGQAEEAIEGLMREVLPNGIGYEWTDLSYQQ
                     RLAGNTAIFIFPLCVILVLLVLAAQYESWTLPLAVILIVPMSLLCAIGGVWLARGDNN
                     IFTQIGFLVLIGLACKNAILIVEFARELHLQGRSVIESALEASRIRLRPILMTSFAFI
                     MGVLPLVFSSGAGAEMRHAMGVAVFSGMLGVTFFGLVLTPVFYVVLQGLVERRTRMAR
                     QFAVQPAHDEDSRHA"
     gene            1393..>2127
                     /locus_tag="VHH53_08185"
     CDS             1393..>2127
                     /locus_tag="VHH53_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX2290158.1"
                     /translation="MPKTALFAWLALAVVGCASAPGYRPSQVQLPSAFRETRDTTVEV
                     VTTGAAVPVSWPDLGDTTLTRLVGQLARSNFDVRAAEERVRGARAARTEVALDLAPTV
                     TFAGGYTRQRLAGASFPIGSGTFPDQDIWDAGFDASWELDLFGRVRRNVQAQGAFVGV
                     TQEDLRNIQISLTAELARAYFDLRGAQEQLAVATRNAENQRRTLALTRERLEAGRGTA
                     FDTERAQAQLSFTLASIPNLEARVRQA"
BASE COUNT          355 a          683 c          712 g          377 t
ORIGIN      
        1 tcattcgaac catggcggat atcggaagca agcagccggg tgtcgagaac gcggtgcagt
       61 tccccgggct gtcgatcaac gggttcgtca acgcgccgaa cgcgggaatc gtgttcttca
      121 ccctcaagcc gttcgatgag cggcacgggt cagagcagta cggcctgaac atcgcggggg
      181 cgctcaacgc caagttcgcc ggcatccagg atgcattcgt ggccgtgttt ccgccgccgc
      241 cggtgaacgg tctgggccag gtcggcgggt tcaagctcga gctggaggac cgggccggcc
      301 tgggcgaagc tgcgctcaac gaagcaaccc aggcgctgct cgcccgcgca tggcagactc
      361 cgtcgctggc cggcgtgttc agcggctacc gcgtgaacgt gccgcagctg gacgtcgagg
      421 tggaccggga aaaggtcaag cgagaaggga tcgtgctgac cgatctgttc cagacgatgc
      481 aggcttacct gggatcggtg tacgtcaacg acttcaacaa gttcggacgc acgtaccagg
      541 tgaaggtcca ggccgacgcg caattccggg ctacggccga cgacatcgcc cagctcaagg
      601 tgcggaacgc acagggtgcg atggtcccgc tcggctcggt ggtgcaggtg aaggaatcgc
      661 acgggcccga tcaggccacg cgctacaacg gctatccggc cgccgacatc aacggggggc
      721 cggcgccagg ctacagctcc gggcaggcgg aagaggcaat cgaaggattg atgcgagaag
      781 ttctgcccaa cggaatcggt tacgagtgga ccgatctgag ctaccagcag cggctggccg
      841 gcaacaccgc gatcttcatc ttcccgcttt gcgtgatcct ggtgctgctg gtgctggcag
      901 cgcagtacga gagctggacc ctgcctctgg cggtcattct gatcgttccg atgtcgctcc
      961 tctgcgccat tggcggcgtg tggctcgccc ggggtgacaa caacatcttc acccagatcg
     1021 gcttcttggt gctgatcggc ctcgcctgca agaacgcgat tctgatcgtg gagtttgccc
     1081 gcgagctgca cctgcagggg cgttcggtaa tcgagtcggc gctcgaggcg tcacgtatcc
     1141 ggcttcgccc catcctcatg acctcgttcg ctttcatcat gggcgtgctg cccctggtgt
     1201 tctccagcgg cgccggcgcc gagatgcggc acgcgatggg tgttgccgtg ttctcgggca
     1261 tgctgggcgt caccttcttc ggtctagtgc tcacgccggt gttctacgta gtgctccagg
     1321 gactggtcga gcggcgcacc cgtatggctc gtcagtttgc ggtgcagccg gctcacgatg
     1381 aggactcgag acatgcctaa gacagcactc ttcgcctggc ttgccctggc cgtggttggc
     1441 tgcgcgtcag ctcccggcta ccgcccgtcg caggtccagc tcccgagcgc gttccgggag
     1501 acgcgggaca ccaccgtgga ggtagtcacc accggagcgg ccgtacccgt cagctggccc
     1561 gatctgggag acacgacgct gaccagactg gtaggccagc tggctcgctc caacttcgac
     1621 gtccgagcag ccgaggagcg ggtccgcggt gcgcgtgctg cccgcacgga ggtagcgctc
     1681 gacctcgcgc cgaccgtcac gttcgccggc gggtatactc gccagcgact ggccggtgcg
     1741 tcattcccga tcggctcggg gacctttccc gaccaggata tctgggatgc cggtttcgac
     1801 gcctcctggg aactggacct gttcggcaga gtccgccgaa acgtgcaggc gcagggagcg
     1861 ttcgtcggag tgacccagga agacctgcgg aacattcaga tctcacttac ggccgagctg
     1921 gcccgggcct acttcgatct ccgaggggcg caggagcagt tggccgttgc cacccggaat
     1981 gccgagaacc agcgccgcac tctggccctg acccgggagc ggctcgaggc gggccgcggc
     2041 accgctttcg ataccgagcg ggcccaggca cagctgagct tcaccctcgc ttccattccc
     2101 aacctcgaag cgcgggtacg gcaggca
//
LOCUS       DATADN010000634         1755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_820564, whole genome shotgun sequence.
ACCESSION   DATADN010000634 DATADN010000000
VERSION     DATADN010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820564"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1353)
                     /locus_tag="VHH53_08190"
     CDS             complement(163..1353)
                     /locus_tag="VHH53_08190"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX2290159.1"
                     /translation="MSSASAQRPLPLVRPPALRTGQEASASRLELFFDLAYVLVVLEL
                     ASTFVSDLTWRGLGTFVGLFIAIWFSWVGFTLYANRFDTDDVIFRIAKLAATLAIACC
                     AASASAATSELSTPFALSFLAGRIVLLLLHARAWCHVTQARPTIAVYLSSTAVSTALW
                     AVSLAVDGAPRYLLWAAAVAVDAAGPAVATWQEDRAPLHMEHLPERFGLFVILVLGEA
                     VGGAAIGVHDAKWASASVAVGIAGFVIAGALWWNYFDITASHSADELQDHAEDHGNDD
                     DATVDERHDLFVYGHLPLTAGIVIAGVGIKDLVLHPAATLPSAGGWTLAGGMALYLIG
                     AAVILGGPHRAWRAVWPWPTAAIPVVLGAALVPHHTALLLVGGLGIASVALAILGTVR
                     SRAR"
BASE COUNT          317 a          561 c          642 g          235 t
ORIGIN      
        1 gaggcgaggg gcgtcctcgc catgtaccaa agcagcgagt cagccctctc gggcactctg
       61 gcgaaccgta ataaaccggg catttgaagg cctgctacgt ggcttctggg cgggcaagtg
      121 gatcagttcg ggtggaccga acgctcaatg caacagtgcc cttcagcgag ctctgctgcg
      181 gaccgttccg agtatggcga gtgcgacgga cgcgatcccc aggcctccga cgagtagcag
      241 tgcggtgtgg tgggggacga gggcggcgcc taggacgacc gggatggcag cggtgggcca
      301 cggccagacc gctcgccagg cgcggtgggg gccgccgagg atgacggcgg caccgatcag
      361 gtagagggcc atgccgccag ccagcgtcca gccgccggcg gagggcagcg tggccgctgg
      421 atgcaggacc aggtccttga tgccgacacc agcgatgacg atccccgcgg tgagggggag
      481 gtggccgtag acgaacaggt cgtggcgctc gtcgacggtt gcatcgtcgt cgttgccatg
      541 atcctcggcg tggtcctgga gttcgtctgc gctgtggctg gcggtgatgt cgaagtagtt
      601 ccaccacagt gccccggcga tgacgaagcc ggcgatgccg acggccaccg aggcgctcgc
      661 ccatttcgcg tcgtgtacac ctatggccgc gccgccgacg gcctcgccga gcaccaggat
      721 gacgaacagc ccgaaccgct ccgggaggtg ctccatgtgc agcggcgcgc ggtcctcttg
      781 ccaggtggcg acggcggggc cggcggcgtc gaccgcgacg gccgcagccc acagcaggta
      841 gcggggggcg ccgtcgactg cgagcgacac cgcccatagc gcggtgctca ctgcggtgct
      901 tgacaggtag acagcgatgg tcgggcgggc ctgggtgacg tggcaccacg ctcgcgcgtg
      961 gagcagcagc aggacgatgc ggccggctag gaagctaagc gcgaacgggg tggacaactc
     1021 cgaggtggcg gccgaggcgc tggccgcgca gcaggcgatg gcgagcgtgg cggcgagctt
     1081 ggcgatccgg aagatgacat cgtcggtgtc gaagcggttg gcgtagaggg tgaagcccac
     1141 ccacgagaac cagatcgcga tgaacaggcc gacgaatgtg ccaagaccgc gccaagtgag
     1201 gtcgctgacg aacgtgctcg cgagctcgag cactaccagc acataggcga ggtcgaagaa
     1261 cagctccagc cggctcgcgc tggcctcctg gccggtacgc agggccggcg gcctgaccaa
     1321 gggcagcggc cgttgggcac tggccgagct cacggcgcgc ccctccctcc catcaccgct
     1381 tggccgggca ccaagtacga gtggctcgag ttgccaacca gcatcagcgc cgaccccgat
     1441 gactcccccg gtgatcgcgc gggaccgcga tggtaaccgg gtcggggagc ctcagaggtg
     1501 cagcacaaac caatcccaca gcctttcccg gtcatgacca gcaccgcccg gctccggcca
     1561 ccgacacggg ttgccgtcgg accagcgttt ccatcatcga aaagtaccgc gaaggctcac
     1621 taggaaacgg gggacggtac cgccctgcgc gagcggccca cggagaggac ccacccgagc
     1681 cagtgtcagc cgggatgggc aaatggcgtg cgggacggac agctctggct ggcatacggg
     1741 acacagcagc catcg
//
LOCUS       DATADN010000635         1689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1140611, whole genome shotgun sequence.
ACCESSION   DATADN010000635 DATADN010000000
VERSION     DATADN010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140611"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1128)
                     /locus_tag="VHH53_08195"
     CDS             complement(109..1128)
                     /locus_tag="VHH53_08195"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEX2290160.1"
                     /translation="MVDRTGPRRDLSPRGPGRSFSRGGARDLGIGWRTAMAAVSEHGT
                     PRVDDASRLDGVEVVGLDETAFQAASATRSTSFVTGIVDLTRRRGGSARLLDVVDDRS
                     ASALVSWINQRDRGWRAGIGVAALDPYRGYASALRASLPHAVRVVDAFHVVRLGFTAV
                     DEVRRRIQREQTGHRGRTGDPLYGIRRLLRRAVDHHSERSWARLLAGLDAGDTTDEQL
                     ARAWIAAQDLRLIYHSPDRARAEQALYRWLAYCADADIPELARLAATIDSWRTEMLAY
                     FDTAKVSNGPTEAINLLIKKIKRVGHGFRNFDNYRLRLLLHCGVDWDTVRATPIRGRL
                     PRSVA"
BASE COUNT          307 a          612 c          523 g          247 t
ORIGIN      
        1 accgcccgct tgccgacctg cgcatcgccg aactgggtcc caaagccggc ctcgtcggcg
       61 ccgccgacct ggcccgctac cgggacagtc gtgtcaggta atcaggctct acgccactga
      121 gcgtggtaac cgacctctga tcggggtggc gcggacagtg tcccagtcga cgccgcagtg
      181 gagcagcagg cgcagccggt aattgtcgaa attgcggaat ccgtggccga cccgcttgat
      241 cttcttgatg agcaggttga tcgcttcggt gggtccgttg gataccttgg cggtgtcaaa
      301 gtaggccagc atttcggtgc gccaggagtc gatggtggcc gccagacggg ccagctcggg
      361 gatgtcggca tcggcgcagt aggccagcca ccggtacagc gcctgctcgg cgcgggcgcg
      421 gtcggggctg tggtagatca gccgcaggtc ctgggcggcg atccaagcgc gggcgagctg
      481 ctcgtcggtg gtgtccccgg catccagccc ggccagcagc cgcgcccaag accgctcgga
      541 gtggtgatcg acagcgcggc gcaacaaccg gcggatgcca tacagcgggt cgccggtgcg
      601 gccgcggtgg ccggtctgct cgcgctggat gcggcgacgc acctcgtcga cggcggtgaa
      661 ccccaaccgc acgacgtgga aggcatccac gacgcgcacc gcgtgcggca gactggcgcg
      721 cagcgctgat gcatagccgc ggtagggatc caacgcggcc accccgattc cggcccgcca
      781 gccacggtca cgctggttaa tccagctcac cagcgctgat gcgctgcggt catcgacaac
      841 atccagcagc cgcgccgagc caccccgacg gcgagtcaaa tcgacgatgc cggtgacaaa
      901 gctcgtcgac cgagtcgccg aggcagcctg aaacgcagtc tcatccaggc ccaccacctc
      961 gacaccgtcc agtcggctgg cgtcatcgac ccgcggcgtg ccatgttcgc tgactgcggc
     1021 catcgccgtg cgccacccga tgcccaagtc cctggcaccg ccgcgactga agctccgtcc
     1081 tggcccacgc ggcgacagat ctcggcgcgg gcccgttcgg tcaaccacac ccgcggcgcg
     1141 atcaccgcag aggtctcagt ccaggtccgc ttcgcacaca acacgtgccg acagcgccag
     1201 acccgcttga cccacaccaa cgcgaccggc cgacccccga caggcaggtc tcgcacccag
     1261 cacggccgcc ggtcatgcaa ctgggccacc gccccacact ccggacaacc caccacatcc
     1321 gcggtggtct caatcgcctg ctccacctca ccggcgtaat ccgacacagc cagcacgaca
     1381 aacccggcca agcccaacat cgccgccgcg gcgctacgct ctcgttccac ctggggcctc
     1441 cattcccgtg tccatgaaca ccacggatca tgaggtccca ggcccatctc cggacttagg
     1501 ctgaagatcg tgtcctacag cggcacacca cgctcagaga cgagagccag ttaaggcggt
     1561 aaagcgtcat ttgtcggccg gcaggacgca tatggtgagc gcgctgccct cgatccgccg
     1621 cagtgacttg acaatgatct agggtgcgga cacatgcaac caactcgcaa ttgacttacc
     1681 ggtgcacgt
//
LOCUS       DATADN010000636         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_990316, whole genome shotgun sequence.
ACCESSION   DATADN010000636 DATADN010000000
VERSION     DATADN010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990316"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /gene="narJ"
                     /locus_tag="VHH53_08200"
     CDS             <1..567
                     /gene="narJ"
                     /locus_tag="VHH53_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX2290161.1"
                     /translation="DEGRRHTAEQLLAQLDGPVTEHLWLTMTALRAADPLQAAQDYVA
                     TFDLRRRATMYLTYWTAGDTRNRGGAMLSFAQVYRGAGVAPPADEAPDYLPVVLEFAA
                     TVDPEAGSRLLAEHRVPIDVLHQALSERSSPYAHAVAAVLATLPAATETEMRRARTLA
                     ADGPPAEAVGLQPFTLTVPPRRTGEESS"
     gene            569..1300
                     /gene="narI"
                     /locus_tag="VHH53_08205"
     CDS             569..1300
                     /gene="narI"
                     /locus_tag="VHH53_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX2290162.1"
                     /translation="MSGWEIFWDVAPYVTLAVVAVGIWWRYRYDKFGWTTRSSQLYES
                     RLLRIGSPMFHFGILVVIVGHVIGLFIPESWTYAIGVSQHAYHVQALVLGGIAGVTTL
                     TGIALLIFRRRTTGPVFMATTVNDKVMYLVLVMAIVAGLACTLIGATPVGAEHDYRQT
                     VAPWFRSIWILQPRGDLMALAPLWFHIHVIIALVLFCLWPFTRLVHVFSAPIGYLFRP
                     YIVYRSRDVARKGELVGSHPPRRGW"
     gene            complement(1332..>1613)
                     /locus_tag="VHH53_08210"
     CDS             complement(1332..>1613)
                     /locus_tag="VHH53_08210"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX2290163.1"
                     /translation="IIFCGHFPEDVQEFTVEETKAETRGQWYFRQVLGSANLSGGKLF
                     HLLSGNLSFQIEHHLFPDVPAHRHAELAPAVRDICRRYGLPYNAGPLPR"
BASE COUNT          240 a          546 c          548 g          279 t
ORIGIN      
        1 gacgagggcc ggcgtcacac cgccgaacag ctgctggcgc aactggacgg gcccgtcacc
       61 gaacatcttt ggctcaccat gaccgcgctg cgggccgccg acccgctaca ggccgcacag
      121 gactacgtcg cgaccttcga cctgcgtcgc cgcgccacga tgtacctgac gtactggacg
      181 gccggcgaca cccgcaaccg cggcggcgcg atgctgtcct tcgcgcaggt gtaccggggg
      241 gctggggtgg ccccgccggc cgatgaggcc cccgactacc tgcccgtggt gctggagttc
      301 gccgccaccg tcgaccccga ggccgggtcg cgtctactcg ccgaacaccg cgtgccgatc
      361 gatgtcctgc atcaggcact gtcggagagg tcgtcgccgt acgcccatgc cgtggccgcg
      421 gtactcgcca cgctgcccgc tgccaccgaa accgagatgc gccgggcccg cacgctggcc
      481 gccgacggcc cgcccgccga agccgttggg ctgcaaccgt tcacgctgac cgtgccgccc
      541 cggcggaccg gagaggagag cagctgagat gtcaggatgg gagattttct gggacgtggc
      601 gccgtacgtc accctggccg tcgtggccgt ggggatctgg tggcgttacc gctacgacaa
      661 attcggctgg accacccggt cgtcgcagct ctacgagtca cgcctgctgc gcatcggcag
      721 cccgatgttc cacttcggca tcctcgtcgt catcgtgggc cacgtcatcg gtctgttcat
      781 cccggaatcg tggacatacg cgatcggggt gagccaacac gcctaccacg tgcaggcgct
      841 ggtgctgggc ggtatcgccg gggtcaccac gttgacgggt atcgcgctgc tgatcttccg
      901 gcgccgcacc acaggtccgg tgttcatggc caccaccgtc aacgacaagg tgatgtatct
      961 ggtgctggtg atggcgatcg tcgcggggct ggcgtgcacg ctgatcggcg cgactccggt
     1021 gggcgccgaa cacgactacc gccagacggt cgcgccgtgg ttccggtcga tctggatact
     1081 gcagccgcgg ggcgacctga tggcgttggc gccgttgtgg ttccacatcc acgtgatcat
     1141 cgcgctggtt ctgttctgtt tgtggccgtt cacccggctg gtacacgtgt tcagcgcacc
     1201 gatcggttac ctgttccgcc cctacatcgt ctaccgcagc cgcgacgtcg ctcgcaaggg
     1261 tgagctggtc ggatcgcatc cgccccgccg gggctggtga cctgaccgcg gcgtctcggc
     1321 ggccggaatc ctcagcgcgg cagcgggccg gcgttgtacg gcaacccgta acggcggcag
     1381 atgtcacgca ccgcgggagc gagttcggcg tgccggtggg cgggaacgtc cgggaacagg
     1441 tggtgttcga tctggaacga caggttgccc gacagcaggt ggaagagctt gcctccactg
     1501 aggttcgccg aaccgagcac ctggcggaag taccactggc cgcgggtttc ggccttggtc
     1561 tcctcgaccg tgaactcctg aacgtcttcg gggaagtgcc cgcagaagat gat
//
LOCUS       DATADN010000637         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_990338, whole genome shotgun sequence.
ACCESSION   DATADN010000637 DATADN010000000
VERSION     DATADN010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990338"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VHH53_08215"
     CDS             <1..1218
                     /locus_tag="VHH53_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX2290164.1"
                     /translation="GSAVGPAMGVVSGNGADLDEVHAVVAASEQAARQSGPARDAAPL
                     VEGGADDDFDAPAAATEIGVFARLADELAAAFEEASGELLYGFAEHRLETTWLASSTG
                     LRRRWVQPTGTVEVNGKIIDDLARSAWTGAYTADFTDVNLTAIIAEVRRRLSWSQRRI
                     ELPAGRYETVLPPSAVADLMIDLVSSMEGRPAHEGRSPWSAAAGSSSPTRLGERLTDL
                     PLTLFSDPVAEGLSTPPFLATTASSDSVSVFDNGEPVHRVNWLRDGAVNALTYPRAAA
                     AQLGGEFTPPPENLVLTGGSSASTDDLVARTERGLLLTCLWYIREVDPTSLLLTGLTR
                     DGVYLIEGGEVVGQVNNFRFNESPIDLLRRVGDVGATEGTLCREAKGWFDRTSMPPML
                     VTDFNMSSVSQAS"
     gene            1409..>3191
                     /locus_tag="VHH53_08220"
     CDS             1409..>3191
                     /locus_tag="VHH53_08220"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX2290165.1"
                     /translation="MTAATIPGIDDAPLNHARLCDWVRDTAELTMPDRVVWCDGSEHE
                     WERLTAQLVDAGTFTRLNRKPNSFWAVSDPSDVARVEECTFICSREERDAGPTNNWMH
                     PDEMKSIMTELYRGCMRGRTMYVVPFCMGPLEDAAPKLGVEITDSPYVVCSMKIMTRM
                     GAAALRKFVRPDGGDADFVPCLHSVGHPLERGDADVPWPCNDIKYISHFPDERLIWSF
                     GSGYGGNALLGKKCYSLRIASVMARDEGWLAEHMLIIKLISPHNRTHYLAVAFPSACG
                     KTNLAMLQPTIPGWKVETLGDDIAWMRFGADGRLYAVNPENGFFGVAPNTSWKTNPNA
                     MRTLQYGNSIFTNVALTDDGDIWWEGMEGTPAHAISWTKQDWTPAEGAAGQRGAHPNS
                     RYCTPMAQCPVLAEEWDDPAGVPISAIIFGGRRADTIPLVNEAFDWQHGVFLGATLSS
                     EKTAAAAGGLGQVRRDPMAMLPFIGYNAGDYFQHWINVGKGADAGKLPKIFYVNWFRR
                     DADQRFLWPGFGENSRVLKWIIERIEGRAAAADTPIGDVTAALAVDVDEWMAELPSIE
                     QWFGKIGDALPSSMRDELDALKWRLGRV"
BASE COUNT          535 a         1010 c         1095 g          551 t
ORIGIN      
        1 ggatccgccg tgggccccgc aatgggcgtg gtcagcggca acggtgctga cctcgacgag
       61 gtgcacgccg tggtggcggc cagcgagcag gccgcccggc agtccggacc agcccgggac
      121 gccgcgccgc tggtggaagg aggagccgac gacgacttcg acgcaccggc ggcagccacc
      181 gagatcggag tgttcgccag gctggctgat gagctggcag cggcattcga ggaggcgtct
      241 ggtgagcttc tctacggctt cgccgagcac cgcctggaga ccacttggct ggcgtcctcc
      301 actggactac gccgccgctg ggttcagccc accggcaccg tggaggtcaa cggtaagatc
      361 atcgacgatt tggcccgcag cgcctggacg ggggcctaca cggctgattt caccgatgtg
      421 aatctcactg cgatcatcgc cgaagtgcgg cgacgtttga gttggtcaca acgccggatc
      481 gaactgcccg cgggccggta cgagacggtc ctgccgccgt ctgcggtcgc tgatctgatg
      541 atcgacctgg tctcgtcgat ggaaggccgt ccggcacacg agggtcgctc gccatggtct
      601 gccgcggccg ggtcgagctc gccgacccgg ctgggtgagc ggctcaccga cctgccgctg
      661 acgctgttca gcgatccagt tgcggaagga ttgtcaaccc ccccgttcct ggccactacc
      721 gcctcaagcg actcggtgag cgtcttcgac aacggcgagc cggtgcaccg ggtgaactgg
      781 ttacgtgacg gtgcagtcaa cgcgctgacc tacccgcggg cggctgccgc acagctgggt
      841 ggggagttca ccccaccgcc ggagaacctg gtgttgaccg gtgggtcgtc ggcgtcgacc
      901 gacgacctgg tggcgcgcac cgagcgggga ttgctgctga cctgcctgtg gtacatccgt
      961 gaggtcgatc cgacctcatt gttgctcacc gggctgactc gggacggggt gtacctcatc
     1021 gagggcggtg aggtggtcgg ccaggtgaac aacttccggt tcaacgagag cccgatcgac
     1081 ctgctccggc gcgtcggtga tgtcggcgcg accgagggca ccctgtgccg ggaggcaaag
     1141 ggctggttcg accgcacctc gatgcccccc atgctcgtaa cggacttcaa tatgtcctcg
     1201 gtgagccagg ccagctagct cactgggctg tcagcagccc tcctcttcgc cgagggtgcg
     1261 agcggttgct gaccggtttt gtgagggatt tctcaatcaa gggtcgtggt gtgcgctcgg
     1321 gactagcctg gtagcgatcc ttttgacgtc tcaccgcccg tgctgcacat gggcggtact
     1381 cggcgttccc gcaggtagga gacgagccgt gacggcagca accattcccg gtatcgacga
     1441 tgccccgctc aaccacgcac ggctgtgcga ctgggtgcgc gacacagcgg agctgaccat
     1501 gcccgaccga gtggtctggt gtgatggttc cgagcacgag tgggaacggc tcaccgccca
     1561 actggtcgac gccggcacct tcacccggtt gaaccgcaag ccgaactcgt tctgggccgt
     1621 ctcggacccg tctgatgtcg cccgggtgga agagtgcacc ttcatctgct cgcgcgagga
     1681 acgcgacgcc ggtcccacca acaactggat gcacccggac gagatgaagt cgatcatgac
     1741 cgagctgtac cggggatgca tgcgcggccg gacgatgtat gtggtgccgt tctgcatggg
     1801 cccgctggag gacgctgcac ccaagctggg cgtggagatc accgattccc cctacgtggt
     1861 ctgctcgatg aagatcatga cccggatggg cgctgcggca ctgcgcaagt tcgtccgacc
     1921 cgacggcggc gacgcggact tcgtgccgtg cctgcactcg gtgggccacc cgctggagcg
     1981 tggtgacgcc gatgtgccgt ggccgtgcaa cgacatcaag tacatcagcc acttccccga
     2041 cgagcggctg atctggagct tcggctccgg ctacggcggc aacgcgctgc tgggcaagaa
     2101 gtgttactcg ctgcggatcg cctcggtgat ggctcgagac gagggctggc tcgccgagca
     2161 catgctgatc atcaaattga tcagcccgca taaccggacc cattacctcg cggtcgcgtt
     2221 cccgtcggcc tgcggcaaga caaacctggc gatgttgcag ccgaccatcc ccggctggaa
     2281 ggtcgagacg ctgggcgatg acatagcctg gatgcgcttc ggtgccgacg gcaggcttta
     2341 tgcggtgaat cccgagaacg ggttttttgg cgtcgcgccg aataccagct ggaagaccaa
     2401 tcccaacgcc atgcgcaccc tgcagtacgg caactcgatc ttcaccaatg tcgcgctcac
     2461 cgatgacggc gacatctggt gggagggcat ggagggcacg ccggcgcacg caatctcgtg
     2521 gaccaagcag gattggacac cggctgaggg agcggcagga cagcgcggcg cgcacccgaa
     2581 ctcgcggtac tgcaccccga tggcgcagtg cccggtactc gccgaagagt gggacgatcc
     2641 ggccggtgtg ccgatctcgg cgatcatctt cggtggtcgc cgggctgaca ccatcccgct
     2701 ggtcaatgag gccttcgact ggcagcacgg ggtgttcctg ggcgccacgc tgtccagcga
     2761 gaagacggcg gcggcggccg gtgggctcgg tcaggtgcgc cgcgatccga tggcgatgct
     2821 gccgttcatc ggctacaacg ccggtgacta cttccagcac tggatcaacg tcggcaaggg
     2881 cgccgacgcc ggcaagctac cgaagatttt ttacgtgaac tggtttcgcc gcgacgccga
     2941 ccagcgcttc ctgtggccgg gcttcgggga gaacagccgg gtgctgaagt ggatcatcga
     3001 gcggatcgag ggccgggctg ccgcagccga taccccgatc ggcgacgtga ccgctgcgct
     3061 ggcggtagac gtcgatgagt ggatggccga gctcccgagc atcgagcagt ggttcggcaa
     3121 gatcggtgat gcgctgccca gctcgatgcg tgacgagctc gacgccctca agtggcggct
     3181 agggcgtgtc c
//
LOCUS       DATADN010000638         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_990465, whole genome shotgun sequence.
ACCESSION   DATADN010000638 DATADN010000000
VERSION     DATADN010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990465"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..612
                     /locus_tag="VHH53_08225"
     CDS             268..612
                     /locus_tag="VHH53_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HEX2290166.1"
                     /translation="MADRVLRGSRLGTVSYETDRDHDLAPRRSVHYACPKGHNFEVPF
                     ADDAELPATWECRLHGNESMLSDGVEPEPKKSKPPRTHWDMLLERRSIPELEELLDER
                     LTEVRGRRSRSA"
     gene            complement(642..1457)
                     /locus_tag="VHH53_08230"
     CDS             complement(642..1457)
                     /locus_tag="VHH53_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX2290167.1"
                     /translation="MIDQRGGHVEGVGRTIVVIPTYDEQATLPAVLHRLSQAVPEAHA
                     LVVDDDSPDGTGALADELAAADGRIHVMHRGAKTGLGAAYVAGFRWALQRGYDVVVEM
                     DADGSHAPEDLPRLLHALAGASPGTGADVVLGSRYVPGGRVVNWPQYRQWLSRGGNYY
                     SRVALGVGIHDVTGGFRAYRRPVLECLDLGTVASQGYCFQVDLAWRAVRAGFAVLEVP
                     ITFTEREQGQSKMNGTIVREALWRVTVWGVQHRLERLRRRPDLADSPYGANQS"
     gene            complement(1470..>2719)
                     /gene="lnt"
                     /locus_tag="VHH53_08235"
     CDS             complement(1470..>2719)
                     /gene="lnt"
                     /locus_tag="VHH53_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX2290168.1"
                     /translation="GVATVSRIPAAPVWAAGVWVAAEALIARVPFDGFPWGRVAFGQP
                     TGVFLPVAAIGGAPLLTAAVVLTGFGAGELARRIIRQRRFTTLAIPALLAVAPAIVGL
                     AVAPLAQTAAPEGEAVVAVIQGNVPRAGLDFNAQRRAVLDNHAARTEQLAIDVTAGRY
                     PQPALVIWPENSSDIDPLRNPDARAVIDRAARAIGVPILVGAVLRTDDGHATNTAIVW
                     NPDTGPGQRHDKRRLQPFGEYIPYRDFFRLFSPYADLAGNFVPGNGNGAVDLGSVRVG
                     IATCSEVIFDDLVRQSVQSGAQLLAVPTNNATFGDTDMTYQQQAISRVRAVEHGRAVL
                     VAATSGVSAVIAVDGTVKQHTTLFTPDALVARVPLHSGTTLATRLGPVPEWSLAVLGM
                     LGIAAALVIRHRPNTEIGSDARR"
BASE COUNT          451 a          919 c          917 g          432 t
ORIGIN      
        1 gccctcagtc ctctagagcg ggcaccgcat cgccgccttg gggtagcgac tgctcaaatg
       61 ggtgatgcgg tctccctgtg ccgaagtaac gtgtcacatt gagcctgcga ttcactcttt
      121 gtgggcgatc ttagcgcggg aagggcgcga aggacatcgc cgccacggat cttcggggtc
      181 tgggaacgtc aggcaccctc gcggcgttgc actcggtggg cttgacctag gggacggggg
      241 tcccctacat ggagaaggga catgattatg gccgaccgcg tcttgcgtgg tagccgactc
      301 ggaacagtga gttacgagac ggaccgggat cacgaccttg ctcctcgccg gtcggtgcac
      361 tatgcctgcc cgaagggcca caacttcgag gttccgttcg ccgacgatgc cgaactcccc
      421 gcgacgtggg agtgtcgatt gcacggcaac gagtccatgc tatccgacgg ggtagaaccg
      481 gagccgaaga agagcaaacc gccgcgtacg cactgggaca tgttgttgga gcgacgcagc
      541 attcccgaac ttgaggagct gctcgacgag cgactgactg aagtgcgcgg tcgccgctcc
      601 cgttctgctt gacgtctcac acctgccgtt gcgtgggtgg gtcaggactg attggctccg
      661 tagggactgt cggccaggtc ggggcggcgc cgtaagcgtt ccaggcggtg ctgtacgccc
      721 cagactgtca cccgccacag cgcctcgcgg acgatcgtgc cgttcatttt ggactggccc
      781 tgttcccgtt cggtgaaggt gatcggaacc tctagcaccg cgaagccggc gcggaccgcg
      841 cgccatgcca ggtcgacctg gaagcagtat ccctgggagg ccaccgtacc gaggtcgagg
      901 cattccagta ccgggcggcg gtaggcgcgg aacccgccag tgacatcatg gatgccgacg
      961 cctagcgcca cccgcgagta gtagttaccg ccgcgagaca gccattgccg gtactggggc
     1021 cagttcacca cccgcccacc gggcacatac cgcgagccca gtactacatc ggcacccgtt
     1081 cccggggaag cgccagccag cgcatgcagc agccgaggta gatcctccgg ggcgtgcgag
     1141 ccgtcggcat ccatctccac cacgacgtcg tagccgcgct gcaacgccca gcggaagcca
     1201 gcgacgtagg ctgcgccgag accggtcttg gcgccacggt gcatgacgtg tatccggccg
     1261 tcggcagcgg cgagctcgtc ggccagcgcg ccggtgccgt ccgggctgtc gtcgtctacg
     1321 acaagcgcgt gcgcttcagg taccgcttgg ctcaaccggt gcaggaccgc cggcagggtg
     1381 gcctgttcgt cgtaggtcgg gatcacgacg atcgtccggc cgaccccttc gacgtgccca
     1441 ccccgctgat cgatcatcga cgtcctctct caccggcgcg cgtcgctgcc gatctccgtg
     1501 tttggcctgt gccgtatcac cagggctgcg gctatcccga gcattcccag caccgccagc
     1561 gaccactccg gcacggggcc caaccgagtg gccagcgtag taccggagtg cagcgggacc
     1621 cgtgccacca atgcgtccgg tgtgaacagg gtggtgtgtt gcttgacggt accgtccaca
     1681 gcgatcaccg cactgacccc gctggtggcc gccaccagca ccgcccggcc atgctccacc
     1741 gcgcgcaccc gggagattgc ctgctgctgg taggtcatgt cggtgtcacc gaacgtggcg
     1801 ttgttggtgg gaaccgcgag cagctgggct ccagactgca cgctctggcg taccagatcg
     1861 tcgaagatca cttcggagca ggtggcaatc ccgaccctga ccgacccgag gtccaccgca
     1921 ccattaccgt tgcctggcac gaagttgccc gcgaggtcgg cataggggct gaacagccgg
     1981 aagaagtccc ggtaggggat gtactccccg aagggctgca accggcgctt gtcgtgccgc
     2041 tgccccggcc cggtgtctgg attccagacg atggcggtgt tcgtggcgtg cccatcgtca
     2101 gtgcggagaa cggcgccgac caggatcgga acaccaatag cgcgggccgc ccggtcgatc
     2161 accgctcggg cgtccgggtt gcgcagcggg tcgatgtcgg aggagttctc cggccagatc
     2221 accaaggccg gctgcgggta ccggccagcc gtgacatcga tggcgagttg ctcggtacgc
     2281 gcggcgtggt tgtccagcac ggcgcggcgc tgggcgttga agtccagccc ggcgcggggc
     2341 acgttgccct ggatcaccgc gaccacggct tcgccttccg gcgcggcggt ctgcgccagc
     2401 ggagcaactg ccaagccgac aatcgctggt gccacggcga gcagcgcggg tatcgccagc
     2461 gtggtgaacc ggcgctgccg gatgatccgg cgggccagct ccccagcgcc gaacccggtc
     2521 agcaccacgg cggcggtgag cagcggggcc ccgccaatcg ctgccaccgg aaggaacacc
     2581 ccggtcggct ggccgaacgc cacccgtccc caaggaaagc cgtcgaaggg tacccgggcg
     2641 atcagcgcct cagccgcgac ccataccccg gccgcccaca ccggcgcggc gggtatccgg
     2701 gacactgtgg cgacgccag
//
LOCUS       DATADN010000639         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_990552, whole genome shotgun sequence.
ACCESSION   DATADN010000639 DATADN010000000
VERSION     DATADN010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990552"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VHH53_08240"
     CDS             complement(<1..492)
                     /locus_tag="VHH53_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2290169.1"
                     /translation="MAQLVLGPMLRYVDSTSAVVWVETDEPCRVEVLGVSCPTFALHG
                     HHYGLIELSDLAPGSSLPYTVSLDGEQAWPAPGSDLPPSRIRTIDPDAPIRLVFGSCR
                     TSVPHDVMHNITHGFDVLRCFANELRNTDEPAWPTALIMLGDQVYADEPPETMLEFIR
                     QRRG"
     gene            580..1479
                     /locus_tag="VHH53_08245"
     CDS             580..1479
                     /locus_tag="VHH53_08245"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2290170.1"
                     /translation="MEHDLLIISNAKAGSADQPVLQEVLDVLSRGGTDVHSETLGSPD
                     ELDALLGAHPHRIPVAAGGDGTLHLLVAALHARGELASRVIGLVPLGTGNDFARTVGI
                     PLDPAAAARVVLQGAPHEMDLLTDDAGGVVINAVHLGIGAESAREGEPLKPVLGMLAY
                     RIGALLAGLRSSGWPLEVSVDGATITDGNCRVLQLGVLNGRTIGGGTPLAPHAEPDDG
                     LADVVVSTATGPVARLRYAQLLRHGRHTEHPDVVLARGRTVELAGAPVPINSDGELGD
                     GVSHRTWTVQPRAWRVLAPPAIR"
BASE COUNT          310 a          679 c          685 g          321 t
ORIGIN      
        1 gccgcgccgc tgccggatga actccagcat ggtctcgggt ggctcgtcgg cgtagacctg
       61 atcgccgagc atgatcagcg cggtaggcca cgccggctcg tctgtgttcc gtagctcgtt
      121 ggcgaagcat cgcagcacgt caaacccgtg ggtaatgttg tgcatcacgt cgtgcggcac
      181 gctggttcgg catgagccga acaccagccg gatcggcgcg tccgggtcga ttgtgcggat
      241 gcggctgggt ggcaggtcgc tgccgggcgc tggccacgcc tgctcgccgt ccaggctgac
      301 cgtgtagggc agcgagctgc ccggtgcgag gtcgctgagc tcgatgagcc catagtggtg
      361 cccgtgcagc gcgaacgttg gacaggagac gccaagaacc tcgacccggc acggctcgtc
      421 cgtctcgacc cacaccaccg cgctggtcga gtcgacgtag cgcagcattg gcccaagaac
      481 gagctgcgcc attgctacct ccaccgttct tcctgaaatt tgtaccttaa taagcagtat
      541 gtgccagccg tatcggccat agctggttgt ctgagcggta tggagcacga cctgctgatc
      601 atctcgaacg ccaaggccgg cagcgcggac cagcccgtgc tgcaggaggt gctcgacgtg
      661 ctcagccgtg gcggcaccga cgtgcacagc gaaacgctcg gcagtccaga cgagctggac
      721 gccctgctcg gtgcgcatcc gcaccggatc ccggtcgcag ccggcggaga cggcacgctg
      781 cacctgctcg ttgccgcgct gcacgctcgc ggtgagctgg cgagccgggt gatcggtctg
      841 gtgccgctag ggaccgggaa tgacttcgcg cgcacagtgg gcattccgct ggaccctgcc
      901 gcggctgccc gcgtcgtgct acagggcgca ccgcacgaga tggacttgct gaccgacgac
      961 gccggtggcg tggtgatcaa cgcggtgcac ctgggaatcg gtgccgagag cgcccgcgag
     1021 ggggagccgc tgaaacctgt acttgggatg ctcgcgtacc ggatcggtgc gctgctggcg
     1081 gggctgcgca gctcaggttg gccgctcgag gtcagcgtgg atggcgccac aatcactgac
     1141 ggtaactgcc gcgtgctgca gctcggcgtc ctcaacggcc gcacgatcgg cggcggcaca
     1201 ccgctggctc cgcacgccga gccggacgac ggtctggccg acgtggtcgt cagcaccgcc
     1261 accggccccg tggcgcggct gcgttacgcg cagctactcc gccacggcag gcacaccgag
     1321 caccccgacg tcgtgctggc ccggggccgt acggtcgagc tggccggcgc accggtgccg
     1381 atcaacagcg acggtgagct tggcgacggg gtcagccacc ggacatggac cgtgcagcca
     1441 cgggcctggc gggttctcgc acccccggcg atccgctaga caacgatgag cacctgaccg
     1501 gtccgtgtcc cggccgtcat gtcggccttt ccactcggtg gcgaccagga acaaggttcg
     1561 cccgtctgca ccaccgagtg cgcaggagaa gcagccgcgg tccagctcga cggtctgcag
     1621 cacctcgccc ccttcccgta cgcgtactgc gcttttgtgc gggacatcgc cgtaccagat
     1681 tgccgcgtcc gcgtcgacgc agcgtctgcg gtccatccac gtcgtatcct ccgtcgctgg
     1741 aaatcaggcg ggtcctaccc ggtgagacgt cggtggggat cagatctcat cgcaggcgcg
     1801 tacgcaccgc atgacacggc caaccgtctt gctcgccgaa aggcacgacc gccaccggac
     1861 ctctacggtc ggatctcgta gaccaggttg aacgcggtct gcgcggcgcg gcggaaccgg
     1921 gtgaagcccg cctgtgtcac gacgtcgtgg atcgcggcct cgccggcctg tgcaccgagc
     1981 gcgtggccgc cgggc
//
LOCUS       DATADN010000640         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_388072, whole genome shotgun sequence.
ACCESSION   DATADN010000640 DATADN010000000
VERSION     DATADN010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388072"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VHH53_08250"
     CDS             complement(<1..540)
                     /locus_tag="VHH53_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2290171.1"
                     /translation="MRNARSVVTSTKPKPWGMVVAVLIVLVFAVGIFGYLFTRYEDRR
                     AFTPTADNKDPSTQIEGVVTQNYGEANRGHIGPDKRVAYNQSPPFGGPHDATWAACNG
                     VVYDTAVRNENMVHALEHGAVWIAYNPDQITGPALQSLRDRVQGESYIMLSPYPGLDS
                     PISLQSWGHQLKLDSPDDKR"
     gene            683..2329
                     /gene="argS"
                     /locus_tag="VHH53_08255"
     CDS             683..2329
                     /gene="argS"
                     /locus_tag="VHH53_08255"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX2290172.1"
                     /translation="MTPAALAELVRSVAHDVLTGRGLDPAVLPTTVTVERPRDPEHGD
                     YATNLALQTGKRAGVAPRELGGWLAEALAQHPGVRSAEVAGPGFLNLRLVADAQGEIV
                     AQVIAAGARFGAGQDLAGLRINLEFVSANPTGPLHLGAARWAAVGDALGRILTFAGAA
                     VTREYYFNDTGAQIDRFVASLVAAASGQPIPAGGYRGAYIDDIAAEVVRREPGVLQAS
                     DRDEVFRRVGVGLMFDTIKRSLHEFGTDFDVWFHKQSLHDSGAVDAAVTRLKDSGALY
                     FADGAWWLHSSEYGDDKDRPVIKSDGAPAYIAGDIAYYLDKRARGFDLCLYLLGADHH
                     GYIARLKAVAAALGEDPATVEVLIGQLVNLVREGTAARMSKRAGTVVTMEDLVEAVGV
                     DAARYALIRSSHASSIDIDLDLWSKRTNDNPVFYVQYAHARLASLARNAADLGISSKD
                     AQLGLLGHEREGELIRTLGEFPRIVASAAQLREPHRVARYLEQLAGAYHRFYDTCRIL
                     PLGDEQPAPRHAARLALSEATRQVLANGLGLLGVSAPERM"
     gene            2326..>3188
                     /locus_tag="VHH53_08260"
     CDS             2326..>3188
                     /locus_tag="VHH53_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX2290173.1"
                     /translation="MRAHPAGPRHADVLARSSTAGPCPAGPDELHALHPAVWPRGAHR
                     GDDGAVRLAGVEVGDLTETYGTPLFVVDEADFRARCAEHVAAFGAAQRVHYAAKAFLC
                     SEIARWVAEQGMSLDVCTGTELALALHAGFPAHRIAMHGNNKSTAELTAAVAAGVGAV
                     VLDSFLEIARLDDIARRHGMVQPVLIRATVGVEAHTHEFIATAHEDQKFGFSLAAGDA
                     AEAARRVLKSDGLRLVGLHSHIGSQIFDSDGFELAAHRMVGLLAAIRDEHGPDAISEI
                     STLDLGGGLGIA"
BASE COUNT          481 a         1091 c         1091 g          525 t
ORIGIN      
        1 gcgcttgtcg tcggggctgt cgagcttgag ctggtgaccc caggactgca gggagatggg
       61 cgagtccagg ccaggataag gcgagagcat gatgtagctc tcgccctgca ctcggtcccg
      121 cagtgattgc agcgctggac cggtgatctg gtccgggttg taggcgatcc agaccgcgcc
      181 gtgctcgagg gcatggacca tgttctcgtt acgcaccgca gtgtcgtaga ccacgccgtt
      241 gcaggccgcc cacgtcgcgt catgcggacc gccgaacggg gggctctggt tataggcgac
      301 ccgcttgtcg ggaccgatgt gccctcggtt ggcctcgccg tagttctggg tgacgacgcc
      361 ctcgatctgc gtcgaggggt ccttgttgtc ggcggtggga gtgaaggcgc gccggtcctc
      421 gtaccgggtg aacaggtagc cgaagatccc gacggcgaac accaggacga tcaggacggc
      481 gaccaccatg ccccacggtt tgggcttggt cgacgtcacg accgaacggg cgtttcgcac
      541 cgccttgctg ctcttgccac tgggcatgcc ctgcctggcc ccctcccgtg gattcgcgta
      601 cggcggccag tctagggatc catcactagg accatccgac cggttcggcg actaggcccg
      661 acggctccct agactccaag aggtgactcc cgctgcgctc gctgagctgg tccgatccgt
      721 cgcccatgac gtgctgaccg gccgcggtct cgaccccgcc gtgctcccca ccacggtgac
      781 cgtagagcgc ccgcgcgatc ccgagcacgg cgactacgcc accaacttgg cgttgcagac
      841 cggcaagagg gccggagtgg cgcctcggga gctcggtgga tggctcgctg aggcactggc
      901 gcagcatccc ggtgtgcgct cggccgaagt cgccggtcct ggctttctca atctgcggct
      961 ggtagccgac gcccagggcg agatcgtcgc gcaggtaatc gctgccgggg cacgcttcgg
     1021 agccgggcag gatctggccg gtcttcggat aaaccttgaa ttcgtctcgg ccaatcccac
     1081 cggtccgctg cacctgggcg ctgcccgctg ggcggcggtc ggtgatgcgc tgggccggat
     1141 cctcaccttt gcgggcgccg cagtgacccg ggagtactac ttcaacgaca ccggcgcgca
     1201 gatcgaccgg ttcgtcgcct cactggtggc agccgcaagc gggcaaccca tcccggctgg
     1261 cggttaccgt ggcgcctaca tcgacgacat cgccgccgaa gtggtgcgcc gcgaacccgg
     1321 tgtgctacag gcctctgacc gcgacgaagt gttccgccgg gtcggcgttg gcctgatgtt
     1381 cgacacgatc aagcgctcgc tgcacgaatt cggcaccgac ttcgacgtgt ggttccacaa
     1441 gcagtccttg cacgactccg gcgcggtcga tgcagccgtt acccggctca aggactccgg
     1501 ggcgctttat ttcgccgacg gcgcctggtg gctgcactcc tcggagtacg gcgacgacaa
     1561 ggaccgcccg gtcatcaaaa gcgacggagc acccgcctac atcgccgggg acatcgctta
     1621 ctacctggat aaacgagcgc gcgggttcga cctgtgtctt tacctgctcg gcgccgacca
     1681 ccacggctac atcgccaggc tcaaggcggt cgccgccgcg ctgggcgaag acccggccac
     1741 cgtcgaagtg ctcatcgggc agctggtgaa cctggtgcgc gagggcaccg cagcgcggat
     1801 gagcaagcgc gcaggcaccg tggtcaccat ggaggacctc gtcgaggcgg tgggggtgga
     1861 cgccgcgcgc tatgcgctga tccgttcctc gcatgcttcg tcgatcgaca tcgatctgga
     1921 cctatggtcc aagcgcacca acgacaaccc ggtgttctac gtgcagtacg cgcatgcccg
     1981 gctggcgtcg ctggctcgca acgctgccga tctcgggatc agctccaagg acgcccagct
     2041 cgggctgttg ggccacgaga gggagggcga gttgatccgc acgctggggg agttcccccg
     2101 catcgtggcc tcggccgccc agctgcgaga gccgcaccgg gtggcccgct acctggaaca
     2161 gctggccggc gcctaccacc ggttctacga cacctgccgg atacttccgc tgggtgatga
     2221 gcaaccagcc ccgcgccacg ctgcccggct ggcgctgagc gaggcgaccc gtcaggtgct
     2281 ggccaacggg cttggcttgc tcggagtgag cgcaccggag cggatgtgag ggcccacccc
     2341 gccgggccac gacacgccga cgtgctggcc aggtcctcca ccgcaggccc gtgcccagca
     2401 gggcccgatg agctgcatgc cctgcacccg gcggtgtggc cgcgcggggc gcaccgtggc
     2461 gacgacggcg cggtgcggct ggccggggtg gaggtcggcg acctcaccga gacgtatggc
     2521 accccactgt tcgtggtcga tgaggcggac ttccgggccc gctgcgccga gcatgtggcc
     2581 gccttcggcg ctgcacagcg ggtgcactac gcggccaagg ccttcctgtg ctccgagatc
     2641 gctcgctggg tcgccgagca gggcatgagc ctggacgtgt gcaccggcac cgagctcgcg
     2701 ctcgccctgc acgccggttt ccccgcgcac cggatcgcga tgcacggcaa caacaaatcc
     2761 accgccgagc tcaccgccgc cgtggcagcc ggagtcggtg cggtggtact cgattccttc
     2821 ctggagatcg cccggctgga cgacatcgcc cgccggcacg gcatggtgca gccggtgttg
     2881 atccgggcga ctgtcggggt cgaggcgcac acccatgagt tcatcgccac tgcgcacgaa
     2941 gaccagaagt tcgggttctc gctggctgcc ggggacgcag ccgaggctgc ccgtcgcgtg
     3001 ctcaagtccg acgggctgcg gctggtcggg ctgcacagcc acatcggctc gcagatcttc
     3061 gactccgacg gcttcgagtt ggccgcgcac cggatggtcg gtctactcgc ggcgatccgc
     3121 gatgagcacg gccccgacgc catctccgag atatccaccc tggatctcgg cggtgggctg
     3181 gggatcgc
//
LOCUS       DATADN010000641         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_388358, whole genome shotgun sequence.
ACCESSION   DATADN010000641 DATADN010000000
VERSION     DATADN010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388358"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1124
                     /locus_tag="VHH53_08265"
     CDS             60..1124
                     /locus_tag="VHH53_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2290174.1"
                     /translation="MYEKDGEKYFVVDTHSHFWDASRENWVPGAENYAKGWIDCFYGY
                     HQLAPPETHWDYEHYLKVSEEDFMRDVFDEGYVDVAIFQSTYLTEWYKTGFNTAAQNH
                     VLTEKYPDKLITNGRFDPREGDAGLKQLEEDAERFNLRGVKLYTAEWYQGSRGWKLTD
                     PEAYRYLERCQELGIKNIHVHKGPTIWPLDKDAFDAADVDGAATDFPELNFIIEHAGI
                     PRIDDFCYMAVQEPNVYAGCSVVIGALMYARPKFFNRVMGELLSWVGEDRMTFGSDYS
                     IWTPKWQVEGLVDWQMPDDEAFSDYPRPTVATKKKILGLNAAKLYDIEVPVECQPPTA
                     ADEDMAPGEQLATETAGVQA"
     gene            1121..1834
                     /locus_tag="VHH53_08270"
     CDS             1121..1834
                     /locus_tag="VHH53_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX2290175.1"
                     /translation="MTLASRALAALETVRDPELDTSIVELQFVSSCTVASDGVAQVRL
                     RLPTFFCAPNFAFLMVADAYDAVSAVEGVTRAEIVLEDHFAATTINDGVAARAGFVAA
                     FGSEAQSELDELRTTFTRKAMLAGQDRVARPLLAAGTQPADLVGMRLGDVAPSADLDR
                     LRQRRAELGIRSDDDAPLLVDGAGAPVGLEALPLHLRRVRTTRVNIEANTGHCLSLLA
                     TRYGVGSATEEPVAPSKFR"
     gene            1891..2916
                     /locus_tag="VHH53_08275"
     CDS             1891..2916
                     /locus_tag="VHH53_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2290176.1"
                     /translation="MKAVRLHDYHKPPVVEDVPEPTISGPLDVIVKIGGAGVCRTDLH
                     IIEGQWADRSGVALPYTLGHENAGWVHAVGDGVTNVAPGDTVILHPAPSCGLCRACRA
                     GDDMHCSNGDFPGLFRDGGMADFLLTSARACVKLDPSTQPADVAALADAGITAYHAVR
                     KAIPLLYPGTTCVVIGAGGLGHIGIQCLAALTATTIIVVDRNPDALKLAEKLGAQHTV
                     VANGQHVAAVKDLTGGAGAEVVLDFVAEQGAEHDGFEMTRQAGSYYVIGYGGQVQIPT
                     LDIISTERNIIGNIVGTYYDLAELMVLAQAGKVTLHTKTYPLDSALEALDDLDNGRVR
                     GRAILVP"
     gene            2944..3425
                     /locus_tag="VHH53_08280"
                     /pseudo
     CDS             2944..3425
                     /locus_tag="VHH53_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636926.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
BASE COUNT          646 a         1134 c         1122 g          523 t
ORIGIN      
        1 ccctacgcgg gccggtgcgc cacacagatc cacgttccga agcgaaagga cccaccacga
       61 tgtatgagaa ggatggcgag aagtacttcg tcgtcgacac ccactcgcac ttctgggatg
      121 ccagtcggga gaactgggta ccgggcgccg agaactacgc caagggctgg atcgactgct
      181 tctacggcta ccaccagctg gctcccccgg agacacactg ggactacgag cactacctca
      241 aggtctccga agaggacttc atgcgcgacg tgttcgacga gggctacgtc gatgtcgcga
      301 tcttccagtc cacttacctg accgagtggt acaagaccgg tttcaacacc gctgcgcaga
      361 accacgtgct gaccgagaag tacccggaca agctgatcac caacggtcga ttcgacccac
      421 gcgagggtga cgccgggctc aagcagctcg aggaagacgc cgagcggttc aacctccggg
      481 gcgtgaagct ctacacggcg gagtggtatc agggttcccg cggctggaag ctcaccgatc
      541 cggaggccta ccggtacctg gaacgctgcc aggagctcgg gatcaagaac atccatgtcc
      601 acaagggtcc gacgatctgg ccgctggaca aggacgcctt cgacgccgca gacgtcgacg
      661 gcgcggcgac cgatttcccg gagctcaact tcatcatcga gcacgcgggc atcccgcgca
      721 tcgacgactt ctgctacatg gccgtgcagg aacccaacgt ctacgccggc tgctcggtgg
      781 tgatcggcgc cctgatgtac gcccggccga agttcttcaa cagagtgatg ggcgagctgt
      841 tgtcctgggt cggtgaggac cggatgacct tcggatccga ctacagcatc tggacgccga
      901 aatggcaggt cgagggcctc gtcgactggc agatgcccga tgacgaggca ttctcggact
      961 atccccggcc caccgttgcg accaagaaaa agatcctcgg gctcaacgcg gcgaagcttt
     1021 acgacatcga ggtcccggtc gagtgccagc cgcccaccgc ggcggacgag gacatggcac
     1081 ccggcgagca gttggccact gagaccgctg gggtccaggc gtgacacttg cgtcacgcgc
     1141 gctggccgcg ctggagacgg tgcgcgaccc cgaactggac acctcgattg tcgagctcca
     1201 gttcgtgtcg tcgtgcaccg tcgcctcaga tggggttgct caggtgcggc tgcgcttgcc
     1261 gaccttcttc tgcgccccca actttgcgtt cctgatggtc gccgacgcct acgacgcggt
     1321 gtcggcggtg gaaggggtga ccagggcgga gatcgtgctg gaggatcact tcgccgccac
     1381 cacgatcaac gatggggtgg ccgcccgcgc ggggttcgta gctgcgttcg gcagcgaggc
     1441 acaaagcgag ctggacgagc tgcggacgac cttcactcgc aaggccatgc tggccggcca
     1501 ggatcgggtg gcccggccgc tgctcgcggc gggcactcag cccgcggacc tggtgggcat
     1561 gcggctgggt gacgtcgcgc cgtcggctga tctcgaccgc ctccggcaac gccgggcaga
     1621 gctcggcatc cggtccgacg atgacgcgcc gctgctggtg gacggcgccg gagcgcccgt
     1681 ggggctcgaa gcgctgcctc tgcacctgcg gcgcgtccgc acgaccaggg tgaacatcga
     1741 agccaacacc ggccactgcc tcagcctgct cgccactaga tatggcgtgg gctcagccac
     1801 cgaagaacct gtggccccat cgaagttcag atgacacagc gaggggcggc ccacaccgca
     1861 cagtgacgat ccaccccatg aggaggagtc atgaaagcag tccggttgca cgactaccac
     1921 aagccgccag tggtagagga cgtgccggag ccgaccatct ccggaccgct ggatgtgatc
     1981 gtgaagatcg ggggggccgg ggtctgccgc accgacctgc acatcatcga gggccagtgg
     2041 gctgaccgga gcggggtggc gttgccctac acgctgggcc atgagaacgc gggctgggtg
     2101 cacgcggtcg gcgacggggt caccaacgtc gccccggggg acacggtcat cctgcatcca
     2161 gcaccgagct gcgggctgtg ccgcgcctgc cgtgccggcg acgacatgca ctgcagcaac
     2221 ggcgacttcc cgggtctgtt ccgagacggc gggatggccg acttcctgct gacctcggcc
     2281 cgggcctgcg tcaagctcga cccgtcgacc cagccagcgg atgttgcggc gctggccgac
     2341 gccgggatca ccgcctatca cgcggtacgc aaggcgatcc cgctgctcta tcccgggacc
     2401 acctgtgtgg tgatcggggc cggcggcctc gggcatatcg gcatccaatg ccttgccgca
     2461 ttgaccgcga ccaccatcat cgtggtcgac cggaacccgg atgcgctcaa gctcgccgag
     2521 aagctgggtg cacagcacac cgtggtcgcc aacggccagc acgtggccgc ggtgaaagac
     2581 ctcaccgggg gggcgggcgc cgaagtggtg ctcgacttcg tcgccgagca gggcgccgag
     2641 cacgatggct tcgagatgac ccgtcaggcc ggctcctact acgtgatcgg ctacgggggc
     2701 caggtccaga tcccgaccct cgacatcatc tccaccgagc gcaacatcat cggcaacatc
     2761 gtcggcacct actacgacct cgccgagctg atggtcctcg cccaggccgg caaggtcacg
     2821 ctgcacacca aaacctaccc cctcgactcg gccctcgagg cccttgacga ccttgacaac
     2881 ggccgggtcc gtggccgcgc gatcctcgtc ccgtagttct ccaaccacac cgagggagat
     2941 cagatggcca aggaactacg tttcggtgcg gaagcccgca gcctgctgct ggccggggtc
     3001 gaccagctgg cggaagcggt caagtcgaca ctcggaccca agggacgcaa cgtcatcctg
     3061 gagaagatca ccggatcgcc ggtggtcacc aacgacggtg tgacgatcgc ccgtgagatt
     3121 cacctcaagg accagttcga gaacatgggc gctcaactgg tcaaagaagc ggcgatcaag
     3181 accaacgaca tcgtcggcga cggcacgaca accgcgaccg tgctcgcgca ggcgatcgtg
     3241 cgcgagggta tgcatgcgat tgactcgggc ggcaacccag tactgatcaa gcggggtatc
     3301 gacctcgccg tggcacggct ggtgtagcac ctgcagagcg tggcgcaccc ggttgccacc
     3361 gagcacgact acgcccgggt ggccgcgatc tcagccaacg acgacgacat ggtcggagag
     3421 gtgat
//
LOCUS       DATADN010000642         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1141566, whole genome shotgun sequence.
ACCESSION   DATADN010000642 DATADN010000000
VERSION     DATADN010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141566"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VHH53_08285"
     CDS             <1..234
                     /locus_tag="VHH53_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HEX2290177.1"
                     /translation="AHATGIVEIYYGRPRNQTSWELATDAVVRTSSAKEVTGAHRLYG
                     IVEGGDLAYVEQRAMAGHPLQPHVSALLHRIIG"
     gene            601..1050
                     /locus_tag="VHH53_08290"
     CDS             601..1050
                     /locus_tag="VHH53_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2290178.1"
                     /translation="MLSRHTIVLVDTSLRRTLHERGMRMTPQRQLVLDAVRDLEHATP
                     ERICEHVQRRAPAVNITTIYRTLDLLEGLNLVRHTHLGHGAPSYSTQPHKHIHLVCHH
                     CGEVAEIPTEELTELAERLRQRFGFVLDPSHLALSGRCANCIGHDGS"
     gene            1047..2081
                     /locus_tag="VHH53_08295"
     CDS             1047..2081
                     /locus_tag="VHH53_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX2290179.1"
                     /translation="MTSPLLDLPGAVPPPPGSPDAGVAWHHGDPLGEQRAAERDVVVV
                     DRSHREVLAVPGEDRLSWLHTITSQHLLALGEGEHTEVLVLDLQGRVEHHAVASHLGE
                     TVWLDTESGKAEALLAYLTSMVFWSKVAPRDASGELAVLSLLGPATAKVIAEAGQLGG
                     FVRVRPDQTDLLVPRGDVIDTWQRLVEAGATPAGSWAYEAIRVAALRPRLGLDTDERT
                     IPHEVGWIGSAVHLDKGCYRGQETVARVANLGRPPRRMVLLHLESPETLPSTGDPVRA
                     DQRVVGRVGTVATHYELGSIALALLKRSVAPSTPLLAGDAAASVDPDSIPPDTGEPPG
                     RAALRRLKSR"
     gene            2106..>2302
                     /locus_tag="VHH53_08300"
     CDS             2106..>2302
                     /locus_tag="VHH53_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEX2290180.1"
                     /translation="MIEVRPGGHRRIDRVLDPGYVAGLSELALVEVRARRDHAAQEET
                     DLSYLRRLLHARIDIVRAEQRR"
BASE COUNT          372 a          809 c          761 g          360 t
ORIGIN      
        1 gcgcacgcca ccggcattgt ggagatctac tacgggcgac cgcgcaacca gacctcgtgg
       61 gagctggcca ccgatgcagt ggtgcgcacc tcgtcggcca aggaggtcac tggtgcgcac
      121 cggctctacg gcatcgtcga aggcggcgac ctggcctacg tcgagcagcg cgcgatggct
      181 ggccaccccc tgcagccaca cgtttccgcc ctcctgcacc gcatcattgg ctagacctcc
      241 cagttaccgc tggatggcac ctgccccaga cagcagtcag cccccgggct gcccgccgca
      301 agcaacgacg agctcgagcg ggaccaatgc tcgggtaagc ccggggggcc gggtgactgc
      361 cttggattcg ccggccgcct ctcgtcggtc cgtcgttgtc aacaacgccg gcccggagga
      421 ggtggccttc cacggccgtc ggcggctcta gctgacagcc acctcacgag tcctatagcc
      481 gttcaacgtt ggaccacctc ctttcccgtg tacctgtcac gctaaccgcg gcacaaccgg
      541 ctcggcaacg tcaatgcgat ccgcccgagc gtgtcagtac acgtttggac acagcatgcg
      601 atgctgtcta ggcataccat cgtgctcgtg gatacctccc tgcgccgcac gctgcacgag
      661 cggggaatgc ggatgactcc ccagcggcaa ctggtgctcg acgcagtgcg cgacctggaa
      721 cacgccacgc cggaacgaat ctgcgaacac gtgcaacgcc gcgcaccagc agtcaacatc
      781 accacgatct accgcaccct cgacctgctc gaaggtctca acctggtccg gcatactcac
      841 ctcggacacg gggcaccgag ctactccacc cagccgcaca aacacataca cctggtctgc
      901 caccattgtg gggaagtcgc cgagataccc accgaggagc tcaccgagct cgccgagcgg
      961 ttgcggcagc ggttcgggtt cgttttggat cccagccacc tggcgctgtc cggacggtgc
     1021 gcgaactgca tcggccacga cggctcgtga cttcacccct gctcgacctg cccggtgcgg
     1081 tccccccgcc cccaggctct cccgacgccg gggtggcctg gcatcacggt gacccgttgg
     1141 gtgagcagcg cgccgccgag cgcgacgtcg tggtcgtgga ccggtcgcac cgcgaggtac
     1201 tcgcagtgcc gggtgaggac cggttgagct ggctgcacac catcacctcc cagcacctgc
     1261 tcgccctcgg tgagggcgag cacaccgagg tgctggtact ggacctgcag ggtcgcgtcg
     1321 aacaccacgc tgtggcctcc cacctgggcg agacggtgtg gctggacacc gaatccggca
     1381 aggccgaggc gctgctggcc tacctgacct cgatggtgtt ctggtccaag gtggcgccgc
     1441 gggatgcgag tggcgagctg gcggtgctct cgctgctcgg tccggccacc gccaaggtga
     1501 tcgcggaggc cgggcagctg ggtgggttcg tgcgggtccg gccagaccag acagatctgc
     1561 tggtgccacg cggtgatgtc atcgacacct ggcagcggct ggtcgaagct ggtgcgactc
     1621 ccgcgggaag ctgggcctat gaggccatca gggtggcggc actgcgcccg cgactgggcc
     1681 tcgacaccga cgagcgcacc atcccgcacg aggtcggttg gatcggctcg gcggtgcacc
     1741 tggacaaggg ttgctaccgg ggtcaggaga cggtggcccg ggtggccaac ctgggccgcc
     1801 cgccgcggcg gatggtcctg ctgcacctgg aatcgcccga gacactgccc agcaccgggg
     1861 acccggtgcg tgccgatcag cgagtggtcg gccgggtcgg caccgtggcc acccactacg
     1921 aactcggctc gatcgcgctg gcgctgctca agcgctcggt ggctcccagc acaccgctgc
     1981 tcgccgggga tgccgctgcg tcggtggacc cggattcgat ccccccggac accggagaac
     2041 caccaggccg ggccgcacta cgccggctga aatcccggta atccgtcggg aaggattcag
     2101 gtcccgtgat cgaggttcgt cccggtgggc accgccgcat cgatcgagtg ctggatcctg
     2161 gctatgtcgc cggcctgtcc gagttggcgc tggtggaggt tcgtgcgcgc cgagaccatg
     2221 cagcgcagga ggagaccgac ctgtcctacc tgcgccggct actgcatgcc cggatcgaca
     2281 tcgtgcgcgc cgagcaacga cg
//
LOCUS       DATADN010000643         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_63171, whole genome shotgun sequence.
ACCESSION   DATADN010000643 DATADN010000000
VERSION     DATADN010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63171"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VHH53_08305"
     CDS             complement(<1..219)
                     /locus_tag="VHH53_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290181.1"
                     /translation="MTNRGRHVVALHSGSAAETRTFTAYRGRLAEVVLDRGGAVLLFG
                     DHQAGWPAHARWTFRPAGGAGQATIGTTW"
     gene            complement(216..857)
                     /locus_tag="VHH53_08310"
     CDS             complement(216..857)
                     /locus_tag="VHH53_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290182.1"
                     /translation="MRLHEIAVAVQAGADSTEWDACLPLWRAAAGVAGHDAALAAMPL
                     TAIGLDDLPTAELAPFAGLILAGRSDQGLLATMQDKLRGFLDEGRVVVFSGQLTHDWL
                     PGATAFEPGPQLGGKPGPPQLDDHPIFSGVSPDDLGVNFLYSNGRHRLPAGADVISQR
                     ADGTPGAYLDRASTAGTVLLHGGANLLVNATTDSSAARIAPQLIDWVCQEARR"
BASE COUNT          288 a          594 c          593 g          297 t
ORIGIN      
        1 ccaggtcgtg ccgatcgtgg cctgtccggc gcctccagcg ggccgaaagg tccaccgggc
       61 atgcgccggc cagccggcct ggtgatcacc gaacagcaac accgcgccgc cgcggtcgag
      121 caccacctca gcaagccggc cgcggtacgc ggtaaaggtt cgggtctcgg ctgccgagcc
      181 gctgtgcagc gcaaccacat gcctgccgcg gttggtcatc ggcgggcctc ctggcagacc
      241 cagtcgatga gttgcggcgc gatccgagcg gctgagctgt cagtggtggc gttgaccagc
      301 aggttggcac cgccgtgcag cagcacggtg cccgcggtcg aggcccggtc caggtaggcc
      361 ccgggtgtgc cgtccgcgcg ctgcgaaatc acgtcggcgc cggccgggag acggtgccgg
      421 ccgttcgagt agagaaagtt cacaccgagg tcgtcgggcg acacaccgga gaagatcggg
      481 tggtcgtcca gttggggcgg tccaggtttg ccgcccaact gcgggccggg ctcgaaggcc
      541 gtcgcgcccg gcaaccagtc gtgggtcagc tgcccgctga agaccaccac gcgtccctcg
      601 tcgagaaagc cgcgtaactt gtcctgcatc gtcgccagca ggccctgatc gctgcgtcca
      661 gccaggatca gcccggcaaa aggcgccagc tcggccgtcg gcaggtcatc gaggccaatc
      721 gcggtgaggg gcatcgcggc aagcgcggcg tcgtggcctg cgaccccggc agctgcccgc
      781 cagagtggga ggcacgcgtc ccattctgtg ctgtctgcgc cggcctgcac ggcgaccgcg
      841 atttcatgca ggcgcaagta caagtcacgc gatgttcaca ctagttaagg ataggctcac
      901 ctgaatgagg gtaagtttcg cctccgcttt gcacaagaga gtgaatgctt aacgctgagt
      961 ccgccaacca tgcgccagtg ccgcacccag gagccagcgc ctcgccgcgc gtaggcggcg
     1021 aggcggctga gaaatgtttt agccctggct ttcctgttcg gtggccggcg gtgcagcgtc
     1081 atgctcgcct gacgtggctc ggccgtgcga gtccgccagc ttgtcaggcg tgagctcgga
     1141 ggcgtcgatc gctgcgctca tcgacggtga cgagcctctc atatccgcca acgctgctgc
     1201 catgtcgtca ggcgagcccg tgctagccgg ctcactcggc tgggaagatt gctcgctgct
     1261 ctcttcgatc atgagcgaag cgtaactgct cacggctcat agcgcagcag aagtgacgcc
     1321 gatgccaaca cgtgaacgga tgcctgcgtg ccctacgggc ccccggacgg tgcaccgaga
     1381 gcaccagcgg gaggctcggc acacaggagt tcctgctgcg cccgccagaa ctcccgccac
     1441 gcgtcatgga gatcattctg tgctgcatcg agggcctccg ccggcgccgg ggccgcaacc
     1501 catcgacctc ggaccggctc caccacggtc aatgcttcaa gggcgtgcga agcattgcgc
     1561 aagcgctgat gggcttggtg aagccgctgg cgttgctgct ccgagagctc ctctggctta
     1621 cgtctggctt ccacgtctca ccctccgccg gtccctggta gtcaaccgta accccgcgac
     1681 atccgtcgac caccgtgcta cgacggctcg cggggtgtgt cgcgcccttg atcagcgcgt
     1741 tgaacgctca gaaggttgtt gagggtcttg ag
//
LOCUS       DATADN010000644         3407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_682261, whole genome shotgun sequence.
ACCESSION   DATADN010000644 DATADN010000000
VERSION     DATADN010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682261"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..394)
                     /locus_tag="VHH53_08315"
     CDS             complement(29..394)
                     /locus_tag="VHH53_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2290183.1"
                     /translation="MSRVAGAHSIDQVLAQARSRLQRVTPAQAHDALAAGALLVDIRP
                     DWQRAADGEVSGALLVERNHLEWRFDPACDARLPQATGYDLQVIVLCSEGYASSLAAD
                     SLRNLGLHRATDVIGGFHA"
     gene            complement(391..894)
                     /locus_tag="VHH53_08320"
     CDS             complement(391..894)
                     /locus_tag="VHH53_08320"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HEX2290184.1"
                     /translation="MYAVPDLTVPAAGIAGSGGPTSFAMMLAAHPERWRPLVDYRTDT
                     RWYRLLERTEQREVWLLSWLPGQGTDLHDHGAASGAFAIAAGTLTERVVAVKPGRSPV
                     EVTRALAAGHCRAFGPHYIHQVINTGAVPAVSVHVYTPGLSVMNCYQVESGELCHVGM
                     EQPGVDW"
     gene            complement(1105..2283)
                     /locus_tag="VHH53_08325"
     CDS             complement(1105..2283)
                     /locus_tag="VHH53_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2290185.1"
                     /translation="MTQKSVTVDRVEALLSSEGHALLDRLGREDPAVIDSIRLSASLR
                     KQFSPELVAAALTQRELRRAAINKFSKASRMFFTRPGLEQASAELVSRYRARRFADFA
                     VLADLCTGIGGDLISIAGQRETLAVDLDEVHLRMAVLNAEVYGVAAGVRTVHADVRSV
                     DLTGVDAVFIDPARRSGGRRLPTGHSEPPLAWCLGLAQAVPAVGIKLAPGLHRDCVPP
                     GWELEFLADGRDLKEAVAWSPTLATAARRATILPGRHTLTRSDGAPVPVGPPGEFLLD
                     PNPAVTRAGLVEELARATGCWKIDDQIAFLSADHLVHTAFARTLRVIESAPWKEKHLT
                     ARLRALDIGTVDIRRRGLAGDVAQLHRRLKLRGTRRATLVMTRMLDLPWSLICVDEAT
                     "
     gene            complement(2444..>3407)
                     /locus_tag="VHH53_08330"
     CDS             complement(2444..>3407)
                     /locus_tag="VHH53_08330"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX2290186.1"
                     /translation="GTTFAKVLADAGRDVVLWARRAEVADAVAHRQTNPDYLPGVRLP
                     SSLQATEHADEALDEADAVVFAVPSQTLRDNLGGWHKFIRADATLVSLAKGVELGSLK
                     RMSEVVIDVTAVTADRVVVVSGPNLAREIAAEQPTASVVACIDHDRAVAVQHACVTGY
                     FRPYTNTDVIGCELGGACKNVIALACGMAAGLGYGDNTLATLITRGLAEMGRLGVALG
                     ADPMTFAGLAGLGDLVATCGSSLSRNRSFGYRLGSGDTLEQAQLATHGQVVEGVKSCA
                     SIQELAARCGVEVPIADAVRRVCNGGQSPRELAADLLGRKPKPE"
BASE COUNT          610 a         1170 c         1131 g          496 t
ORIGIN      
        1 ggcgctggat agggagacct tgggcagact aggcgtggaa accaccgatc acgtcggtgg
       61 cgcggtgaag gcctaggttg cgcagcgagt ccgcggcgag acttgaggca tagccctccg
      121 agcagagcac gatcacctgc aagtcgtagc cagtggcctg cggcaaccgg gcatcacacg
      181 ctgggtcgaa acgccactcc agatggttgc gctcaaccag taatgcaccc gatacctcgc
      241 cgtccgcggc acgctgccag tccgggcgga tgtccaccag cagggcaccc gcggcaaggg
      301 cgtcgtgagc ctgggcgggg gtaacacgct gcaaccgcga acgcgcctgc gccaacacct
      361 ggtcgatgct gtgtgcgccg gccacgcggc tcaccagtcc accccgggct gctccatccc
      421 gacatggcac agctcgccag attccacctg gtagcagttc atcactgaca gaccaggggt
      481 atagacgtgc acgctcaccg cagggaccgc cccagtgttg atcacttggt gaatgtagtg
      541 cgggccgaag gcgcggcagt gcccggcggc cagcgctcgg gtgacctcta ccggggatcg
      601 gcctggcttg acggcaacca cccgctcggt cagcgtgcct gcggcgatcg cgaaggcccc
      661 cgatgccgcc ccgtggtcgt gaagatcggt gccttgaccg ggaagccagg acagcagcca
      721 tacctcgcgt tgctcggtgc gctcgagcaa ccggtaccag cgggtgtcgg tgcggtaatc
      781 gaccaggggg cgccaccgct caggatgcgc ggccagcatc atggcgaagc tggtggggcc
      841 gccggaacca gcgatgccgg cagcggggac ggtgagatca gggacggcgt acacggggat
      901 cctccgcgga gtcggtgaac cgaccgcgcg ggaccaccac cccgagcagg gtgggtcact
      961 agacaggcgc ggtcggtgaa ggcagaaggt tcaccgcgcc ggacagagcg cactcgccac
     1021 ccggcgcaga tccaccgggg accgccggac gaggaaggga gtgctgctca cggtgatgat
     1081 caaaccagca gatgcccgtc ccggtcaagt ggcctcatcg acacagatga ggctccacgg
     1141 cagatcgagc atgcgggtca tgaccagggt ggcacgccgg gtaccgcgga gcttcaggcg
     1201 gcggtgcagt tgcgcgacgt cgccggcgag gcctcgacgg cggatgtcga cagtaccgat
     1261 gtccagtgcg cgcagccggg cagtgagatg cttctccttc cacggggccg actcgatcac
     1321 ccgcagggtc cgggcaaagg cggtgtgcac cagatggtca gcggacagga acgcgatctg
     1381 gtcatcgatc ttccagcatc cggtggcccg ggctagctct tctaccagtc cagcacgggt
     1441 caccgcggga ttggggtcaa gcaggaactc accgggcggg ccgaccggta ccggtgcacc
     1501 atcgctgcgc gtgagggtgt gcctgccggg caggatcgtc gcccgtcggg ccgcagtagc
     1561 cagcgtggga gaccatgcga cggcctcttt caggtcgcgc ccgtcggcga ggaactccag
     1621 ctcccagccc ggcgggacgc aatcccggtg caaccccggt gccagcttga tgcccaccgc
     1681 ggggacggcc tgcgccaggc cgaggcacca ggcaagggga ggctcactgt ggccagtcgg
     1741 cagccgccgc ccgccactgc gccgggcagg gtcgatgaat accgcgtcaa ctccagtcag
     1801 gtccaccgac cgcacgtcag cgtgcacggt acgcacgccg gcagcgacgc cgtacacctc
     1861 ggcattgagc acagccatcc tcagatgaac ctcgtcgagg tccacggcta gcgtttcgcg
     1921 ctggccggct atcgagatca ggtcaccccc gatcccggta cacaggtcgg ccagcacagc
     1981 gaagtcagcg aaccggcgcg ctcgatagcg tgataccagc tcagcggaag cctgctccag
     2041 gccgggacgg gtgaaaaaca tccggctggc tttgctgaac ttgtttatgg ccgcacggcg
     2101 cagctcgcgt tgagtcaacg ccgccgccac cagctccggt gaaaactgct tacgcagcga
     2161 ggcactcagc cgtatcgaat cgatcaccgc gggatcctcg cgaccgagcc gatccagcag
     2221 tgcgtggcct tccgacgaca gcaacgcctc aacgcggtca acggttaccg acttctgtgt
     2281 cacgcgccga ccgtctcgca tcgtcagtgc atccttggca cggctagctg ggaagctgcc
     2341 gtcaccacgc cccggcgggt gccgatggca cggcgtgaga tcgcaggccc gaccgccccc
     2401 gagtgcccgc cggcattgag ctgctgctga gagatggcca ccctcattcc ggtttgggtt
     2461 tgcgaccaag caggtcagct gccagctccc gaggagactg accgccgttg cacacccggc
     2521 gcaccgcgtc ggcgatcggc acctcgacgc cgcaacgcgc ggccagctcc tggatcgatg
     2581 cgcaggactt gacaccttcc actacctgac cgtgggtggc cagctgggcc tgctcaaggg
     2641 tgtctcccga cccgagccgg tagccgaaag aacggttgcg agacagtgat gagccgcagg
     2701 tggccaccag atcgccaaga ccggccaggc ccgcgaaagt catcgggtcg gcgcccagcg
     2761 ccacacccag ccgccccatc tcagccagcc cgcgcgtgat caacgtcgca agcgtgttgt
     2821 ccccgtatcc caaaccagct gccatcccac aagccagcgc gatgacgttc ttgcatgcgc
     2881 cgccgagctc gcaaccgatc acgtcggtgt tggtgtaagg ccggaagtag ccggtcacgc
     2941 aggcgtgctg caccgcaacg gcacggtcgt ggtcgatgca ggcgacgacg ctcgcggtgg
     3001 gctgctcagc ggcgatctcc cgagccaggt tcgggccgga caccaccacc acccggtcgg
     3061 ccgtcaccgc agtgacgtct atcacgacct cgctcatccg cttgaggctg cccagctcga
     3121 cgcccttggc caggctcacc aaggttgcat cggcacggat gaacttgtgc caaccaccca
     3181 ggttgtcccg tagcgtctgg cttggtaccg cgaacaccac cgcgtctgcc tcgtccaaag
     3241 cctcgtcggc gtgttcggtg gcctgcaagg acgacggcaa ccgcactccg ggcaggtagt
     3301 ccgggttggt ctgtcggtgc gcgacggcgt cggccacctc agcccgacgg gcccagagca
     3361 cgacatcccg gccggcgtcg gcgagcacct tggcgaaggt ggtaccc
//
LOCUS       DATADN010000645         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1441864, whole genome shotgun sequence.
ACCESSION   DATADN010000645 DATADN010000000
VERSION     DATADN010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441864"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VHH53_08335"
     CDS             <1..447
                     /locus_tag="VHH53_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2290187.1"
                     /translation="GLHTKEQSLAYAHQTGIVCADEVLLHPDPYPSREAWCQARIAET
                     KRRLIACDPDVPLVLVNHWPLIREPTRVLRYPEFAQWCGTELTADWHRRFNTAAVVYG
                     HLHIPCSTVHDGVRFEEVSLGYPREWTPRGLPRPVLRPILPTQESR"
     gene            complement(497..1342)
                     /locus_tag="VHH53_08340"
     CDS             complement(497..1342)
                     /locus_tag="VHH53_08340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2290188.1"
                     /translation="MNEQAVAQHYSRGGLEQQILDLLTTIGADLEYLDLDQLAPVDEF
                     HIGGRSATAELVNQLDLRPGLRVLDVGSGLGGTARYLARQHAVEVTGLDLTMEYVQVA
                     ASLTRRVGLANLAEFHQGSAAKLPFSDGSFDRACMLHVGMNIADKATLFAEVRRVLVP
                     GGLFGVYDVMRTGPGDIAFPVPWAASAATSFVAEPAYYREVLAKTGLTVQSQRDRRDF
                     ALDFFREMRARTAQGGPPVLGLHIVMGPDAPLKIANMVDGLTRAVLAPTEMICLAEPL
                     LPSVG"
BASE COUNT          344 a          599 c          568 g          303 t
ORIGIN      
        1 ggcctgcaca ccaaggagca atcgctggcc tacgcgcatc agaccgggat cgtctgcgcc
       61 gacgaggtct tgttgcaccc agatccgtat ccgagccggg aagcatggtg ccaggccagg
      121 atcgccgaga ccaaacgacg gctcatcgcc tgcgatccgg acgtgccact ggtgttggtc
      181 aaccactggc cgctgatccg cgagccgacc cgggtgttgc ggtatccgga gtttgcccag
      241 tggtgcggga ccgagctcac cgcagattgg caccgtcggt tcaataccgc agccgtggtg
      301 tacggccacc tgcatatccc gtgctccacg gtgcacgatg gggtgcggtt tgaggaggtg
      361 tcgctgggct atccgcgtga gtggaccccc aggggcctgc cgcgtcccgt gctccgcccg
      421 atactgccga cgcaggagtc caggtgatcg gaactgctca acgcggtggc ctggagtgcg
      481 cgtgcggagc tgacgctcag cccactgacg gcaagagggg ttcggcgaga cagatcatct
      541 cggtcggagc tagtacggcg cgggtcagac cgtcgaccat attcgcgatc ttcaacggtg
      601 cgtctgggcc catgacaatg tgcaagccga gcacgggcgg cccgccctgt gcagttcgtg
      661 ccctcatctc gcggaagaaa tcgagcgcga agtcccgccg gtcacgttgg gactgcaccg
      721 tgaggccggt cttcgcgagc acctcccggt agtacgccgg ttcagcgacg aagctggttg
      781 cagcactagc ggcccacggc acgggaaagg cgatgtcgcc tggtccggtg cgcatcacgt
      841 cgtagacacc gaagagccct ccgggcacca gaactctccg gacctcggcg aacagggtcg
      901 ccttgtcggc aatgttcatc ccgacgtgca gcatgcatgc gcggtcgaat gagccatcgg
      961 aaaacggcag ttttgccgcg ctgccttggt ggaactcggc aagattggcg agcccgaccc
     1021 gacgggtgag cgacgcggca acctgcacat attccatggt caaatcgaga ccggtcacct
     1081 cgacagcatg ctgacgagcc aggtaacgag cagtaccacc caacccgctg cccacgtcca
     1141 gcacgcgcaa ccctggtcgg aggtcgagct ggttgaccaa ctctgccgtg gccgaccggc
     1201 caccgatgtg gaactcgtcg accggtgcca gttgatcgag atccaggtac tcaaggtcgg
     1261 caccgattgt cgtcagcagg tcaagaatct gctgctccaa ccctccacgc gagtagtgct
     1321 gagcgacggc ctgctcgttc atcccaccac ctccggtaca catgatgacc cgaagtggct
     1381 ggagtacacg tcgcatcccg gatccagcgc accgctgcgc gaattgcact ggagaccaca
     1441 tcccggcact ggtcataatc attcgcggca tgcgcgtcgg ggcgaatgcc agataccacg
     1501 gcgtctgcgc caatacgtcc ccttcaagcg cggtcgcacg acaccccggt ggatgcccgc
     1561 gtacgcgcca cgacggctgg tcacctacct tgatcgcgcc caccaggtca cccaggtgtg
     1621 agcacggaca cggacaccgc agcgcagtgg cgatgacctg gcggtgttga cccccgggtt
     1681 tgccgcacac cgacgaaata gcaccgatca gcaacctcgc ccagccgccg gacgcctgcc
     1741 cgtgatccgg gctatatcgt gcaaagcgtg actgtgaatg tgagtagtga cttccccgcc
     1801 gacatcggcc tgga
//
LOCUS       DATADN010000646         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1441901, whole genome shotgun sequence.
ACCESSION   DATADN010000646 DATADN010000000
VERSION     DATADN010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441901"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1425)
                     /locus_tag="VHH53_08345"
                     /pseudo
     CDS             complement(101..1425)
                     /locus_tag="VHH53_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            1506..2054
                     /locus_tag="VHH53_08350"
     CDS             1506..2054
                     /locus_tag="VHH53_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX2290189.1"
                     /translation="MTAPHRMSAATAPQIHPPKVELFDLDAMTNTDPKGVVRTVGEYR
                     VEPFGLYLARDVDDHPSIRALESWLLPEVGLRITDWFYRPGHERDQDFYVDVVRIDID
                     SGRWRTEDHYLDLIVRVGRGVDVLDTDELLDAVLAGLLDPPTAQAALATTYRTVDGLA
                     RHGYRLDRWLSTVGAWPTWSRH"
     gene            complement(2067..>2735)
                     /gene="coaE"
                     /locus_tag="VHH53_08355"
     CDS             complement(2067..>2735)
                     /gene="coaE"
                     /locus_tag="VHH53_08355"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX2290190.1"
                     /translation="CLHNTGSRDETFAAVDQLWAQRLVPFEDNLRHRRRAPRPPGPIL
                     VAPDESWPRQAQRVMARVAAVAAGRAVRIDHIGSTSVPGLDAKDVLDVQVVVADLEVA
                     KQLADDLLGAGLIRLTGHWWDNAPDGGTWDKAIAVNADPARAVNCHIRPIDSPAWRQT
                     VLLRDWLRAHPSSARKYAEVKRRLAARPWDSIDAYADAKTPFVHAALDRAQHWAARTG
                     WRVE"
BASE COUNT          445 a          896 c          915 g          479 t
ORIGIN      
        1 atcggcgctg gataaccggc cgctgacgtg ggaggagttc caggacagga tcggccagac
       61 ggtgtacctg tcggcgaccc cgggaccgta tgagatgggt caggccggtc ggtcggatga
      121 cctgctcgac gaactcgcca ccggcctgac ccatctcata cggtcccggg tcgccgacag
      181 gtacaccgtc tggccgatcc tgtcctggaa ctcctcccac gtcagcggcc ggttatccag
      241 cgccgaaggc agccggaagc cgtgctccac cagagtgcgc ttgcgggaag cgtcgccttc
      301 gtacatcccg ccgatctgcg gcaccgtgac atgcgattcg tcgatgacca gcaggaagtc
      361 ctcggggaag tagtccagca gggtggccgg tgccgagccg gcggggcgtc cgtcgatgtg
      421 ccgcgagtag ttctcgatac cgttgcagaa accgacctga cgcatcatct ccacgtcgta
      481 gtgggtccgc atccgcagcc gctgggcctc cagcagcttg ccctgccgct ccatcgcggc
      541 cagctgatct cccaactcgg catcgatgcc catgatggcg cgttccatcc gctccggccc
      601 ggcgacgtaa tgcgtggccg ggaaaatccg cagctcggtg acctcgcgga ccacctcccc
      661 ggtcagcggg tgcaggtaaa aaagcgactc cacctcatca ccgaagaagt cgatgcggac
      721 cgcaagttcc tcgtaggccg gaatcacctc gacagtgtcg ccacggaccc ggaacgtgcc
      781 gcgggcgaac gcgagatcgt tgcgggtgta gttgataccg accagagccc gcaacagcgt
      841 gtcgcgcccg acctcaccgc cgacgggcac atggatcgac tggctcaggt actcctgggg
      901 ggtaccgagg ccgaagatgc aggacactga ggcgacgaca acgacgtcgc ggcgggtgag
      961 cagcgacatc gtggcagagt gccgcagccg ctccacgtca tcgttgatcg aagagtcctt
     1021 ctcgatgtag gtgtcggtct gcgggacgta tgcctcgggt tggtagtagt cgtagtagga
     1081 gacgaagtac tccaccgcgt tgttcgggaa gtaatcccgc agctcgtggg cgagttgcgc
     1141 ggcgagggtc ttgttcggcg ccatcaccaa cgtcgggcgc tgtacccgtt cgatcagcca
     1201 ggcggtggtg gccgacttac ctgttccggt ggcgcccagc agcacgacgt cacgctcacc
     1261 ggcggtgatt cgacgttcca gctcatcgat agcggctggc tggtcgccgg caggcgcgta
     1321 gtcactgacc atctggaacc ggccttgcgt ccgcggtgtc ttgccgagcg ggcggaactc
     1381 ggagtgcgga cgcagatcgc gttggatggc ggcggcatcg gtcatggcat tgagggtagg
     1441 ccgggtccct gacagcctgc cttcgcctgg tgccctctgc cgggacatgc gtagggtcgg
     1501 cctctatgac ggccccgcac cggatgagtg ccgcgaccgc cccgcagata cacccaccta
     1561 aggttgaact gttcgacctg gacgcgatga ccaacaccga tcctaagggg gtcgtccgca
     1621 cggtcggcga gtaccgggtc gagccgttcg gtctctacct tgcccgtgat gtcgacgatc
     1681 acccgtcgat ccgcgcgctt gagtcgtggt tgctgcccga ggtggggttg aggatcaccg
     1741 actggttcta tcgccccggg catgagcgcg accaggactt ctacgtcgac gttgtgcgca
     1801 tcgacatcga ctccgggcgt tggcgcactg aggaccacta tctggatctc atcgttcggg
     1861 ttggccgagg cgtcgacgtg ctcgacaccg acgagttgct ggacgcggta ctcgccgggc
     1921 tgctcgatcc gcccactgcg caggcggcct tggctaccac ttatcgaacg gtggacggtc
     1981 tggcccggca cggttaccgg ttggaccggt ggctgtcgac ggtgggtgcg tggccgacct
     2041 ggagccggca ctgacgcagc cagcagtcac tccacccgcc agccggttcg cgctgcccag
     2101 tgctgcgccc gatccagagc tgcgtgcacg aacggcgttt tcgcgtcggc gtaggcgtcg
     2161 atgctgtccc agggccgtgc tgccaatcga cgcttgacct cggcgtactt gcgagcactt
     2221 gacggatggg cccgcagcca gtcccgcagc agcacggttt gccgccaggc cggggagtcg
     2281 atgggccgga tgtggcagtt gacggcccgc gccggatcgg cgttgaccgc tatcgccttg
     2341 tcccaggtgc cgccatccgg ggcgttgtcc caccagtggc cggtgagcct gatcaacccc
     2401 gcacccagca ggtcatcggc cagttgcttg gccacctcga ggtcggcgac gaccacctgg
     2461 acatccagca catccttggc gtccagacca ggcaccgagg ttgagccgat atggtcgatg
     2521 cgtactgctc ggccggcggc cacagctgcg acacgtgcca tcacccgctg cgcctgccgc
     2581 ggccaactct cgtcgggtgc aacgaggatc gggccgggag gccgcggtgc ccggcggcga
     2641 tgccggaggt tgtcctcgaa gggcaccagc cgctgtgccc acagctgatc cactgcagcg
     2701 aaggtctcgt cccgagaacc ggtgttgtgc aggca
//
LOCUS       DATADN010000647         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_221010, whole genome shotgun sequence.
ACCESSION   DATADN010000647 DATADN010000000
VERSION     DATADN010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221010"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VHH53_08360"
     CDS             complement(<1..163)
                     /locus_tag="VHH53_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2290191.1"
                     /translation="MSTSGRGLHGRVLETLGPAITAGEYPPGSVLRTDELAQRFDVSR
                     SVMREAVRVL"
     gene            302..820
                     /locus_tag="VHH53_08365"
     CDS             302..820
                     /locus_tag="VHH53_08365"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005168464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX2290192.1"
                     /translation="MNTPHVVVVMGVAGTGKTTIGPLLAARLGVPYAEADDFHPPANI
                     AKMSAGTPLTDEDRRPWLDAIGDWAHGRAGLGGVVSCSALKRSYRDRLRAAAPGVVFL
                     HLTGDRALIEDRMSHRQGHFMPTALLDSQFATLQPLEADEAGVGVDVSGSPEEITERA
                     ASALEGLPEPIQ"
     gene            897..>1688
                     /locus_tag="VHH53_08370"
     CDS             897..>1688
                     /locus_tag="VHH53_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX2290193.1"
                     /translation="MLAADAPEPITSAGHAQLGIAVLAGIAVIVLLITKSKLHAFLAL
                     TIGSLALGAIAGAPLDKVLTSFSTGLGTTVAGVGVLIALGAMLGKMLADSGGADQIVD
                     TILAKAGGRSMPWAMVLIASVIGLPLFFEVGIVLLIPVVLMVAKRGNYSLMRIGIPAL
                     AGLSVMHGLVPPHPGPLVAIDAVQADLGVTLALGVLVAIPTVIIAGPLFSRYAARWVD
                     VPAPDRMIPQRASEDLEKRPGFGATLFTVLLPVILMLAKALVDIVV"
BASE COUNT          241 a          602 c          571 g          274 t
ORIGIN      
        1 ccaggacgcg gaccgcctcg cgcatcaccg agcgtgagac gtcgaaacgc tgtgccagtt
       61 cgtcggtgcg cagaacgctg cccggcgggt actcgccggc ggtgatggcg gggccgaggg
      121 tctccagtac ccggccgtgc agcccccggc ccgatgtgct catgcactca gagtacgtgg
      181 cagatcaccg agccaaaaag tcagacttat tcgctacggg ctcttgaatt catcgtacgt
      241 aatgggtttc atttgcgtcg acatcacgtg tcgacctcgg acagcagaca gtgaggcagc
      301 gatgaacacc ccccacgtcg tcgtggtgat gggcgtcgcg ggcaccggca agaccaccat
      361 cggtcccctg ctcgcggccc gactcggcgt tccgtacgcc gaggccgacg acttccaccc
      421 gccggccaac atcgccaaga tgtcggccgg taccccgctc accgacgagg accgccggcc
      481 ctggctggac gccatcggtg actgggcgca cgggcgggcc ggactcggcg gggtggtcag
      541 ctgctcggca ctgaagcggt cgtaccgcga ccggttgcgg gccgccgcgc ccggcgtggt
      601 cttcctgcac ctcacgggtg accgtgcgct catcgaggac cggatgtcgc accggcaggg
      661 gcacttcatg cccaccgcgc tgctggactc ccagttcgcg acgctccagc cactggaagc
      721 ggacgaggcg ggggtcggcg tggacgtctc gggcagcccg gaggagatca ccgagcgggc
      781 cgcgagcgcg ctcgaggggc tccccgagcc gatccagtag cgctttgccg gcttcctctc
      841 cccccaagtc cccgtaaccg caagggaacc ccaccgtgac cagactcagc gtcgagatgc
      901 tggcagcgga cgcacccgag ccgatcacct cggccggcca cgctcagctg ggcatcgccg
      961 tcctggcggg catcgccgtc atcgtcctgc tcatcaccaa gtccaagctg cacgcgttcc
     1021 tggcgctgac catcgggtcg ctggcgctcg gcgcgatcgc cggggcaccg ctggacaagg
     1081 tcctcaccag cttcagcacc ggcctcggca ccacggtcgc cggcgtcggc gtgctgatcg
     1141 ccctcggtgc gatgctcggc aagatgctcg ccgactccgg gggtgccgac cagatcgtcg
     1201 acacgatcct cgcgaaggcc ggcgggcggt cgatgccgtg ggcgatggtg ctcatcgcct
     1261 cggtgatcgg actgccgctg ttcttcgagg tcggcatcgt gctgctgatc ccggtggtgc
     1321 tgatggtcgc caagcgcggc aactactcgc tgatgcggat cggcatcccg gcgctcgcgg
     1381 gcctgtccgt gatgcacggc ctggtgccgc cgcacccggg tccgctggtc gcgatcgacg
     1441 cggtccaggc cgacctcggc gtgacgctgg cgctcggtgt cctcgtcgcc atacccacgg
     1501 tgatcatcgc cggcccgctg ttctcgcggt acgcggcccg ctgggtggac gtccccgccc
     1561 cggaccgcat gattccgcag cgtgcttcgg aggacctgga gaagcgcccc ggcttcggcg
     1621 ccacgctgtt cacggtgctg ctgccggtga tcctgatgct ggccaaggcg ctggtcgaca
     1681 tcgtcgtg
//
LOCUS       DATADN010000648         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_683630, whole genome shotgun sequence.
ACCESSION   DATADN010000648 DATADN010000000
VERSION     DATADN010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683630"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..844
                     /locus_tag="VHH53_08375"
     CDS             17..844
                     /locus_tag="VHH53_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290194.1"
                     /translation="MLVVGGHLSLLAVTVLVGAVFPTLATAALLTTRPARAALTEHAW
                     AEPHQHTARALGTLAATALTLALLNPPVARNTALPEVPANWVDKTTLHDPVAYHFVTR
                     YAGPDATFVRYHTAAQPGFPALAVDVLSSPHRSALTDTADVIWYPSSRPVDYRAVTSR
                     PGLPTGSRIAYSNADAATDGTRSDWFVVTWEWQAGNVFQRVNVVASQSLTGDRLPPEP
                     QPVDFLDVSLRPALWVARQQPNNPGDVDDVVVERAMTLAGVLGGTAAGRTDGGVADA"
     gene            837..>2232
                     /locus_tag="VHH53_08380"
     CDS             837..>2232
                     /locus_tag="VHH53_08380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2290195.1"
                     /translation="MREPTPVVLPAHLSARGLLGRRALLLLAAVVAVHAAAVANWPHI
                     VAPVLTGIFALVYLVSTLDRNYLLLKGLHSNALVSISAEEALAIPDHELPVYTVLLPV
                     YDEPTIVANLINGVGRLDYPADKLEILLLVEEDDLATQEALLGADMQSVRIVIVPHSQ
                     PKTKPKACNYGMSLPDLRGEYLTIYDAEDIPDPLQLRRAVAAFRRVPSDIGCLQGRLG
                     YFNENQNLLTRFFSLEYDQWFGVVLPAVEQARCVVPLGGTSNHMPTEVWRAVGGWDEF
                     NVTEDADLGVRLARSGYRTMILDSITLEEANSDVVNWIRQRSRWYKGYLQTMLVHLRD
                     PRGLHRAIGTKAFLRMLNMTGGVPLTSALNVLFWFTMLMWIVGRPVVIGMLFPPLTYY
                     VCLSLLLICAPLSVFTGLIVTQALGKPHLWVAALLSPLYWVLQSVAAIKAIYQLVFRP
                     SFWEKTIHGLSPSRE"
BASE COUNT          332 a          830 c          699 g          371 t
ORIGIN      
        1 ggcgtcgggg ctcacggtgc tcgtcgtagg cggccacctg tcgctgctgg cggtcaccgt
       61 cctcgtcggt gccgtgtttc ccacgctggc gacagccgcc ctgctgacga cgcggccggc
      121 gcgcgcggcc ctgaccgagc acgcgtgggc cgaaccgcat cagcacacgg cgcgcgcgct
      181 cggcacgctc gcggccaccg ccctcacctt ggcgctcctc aacccgccgg tcgcccggaa
      241 caccgcgctg cccgaggtcc ccgccaactg ggtcgacaag acgaccctgc acgatcccgt
      301 cgcctaccac ttcgtcaccc gttacgccgg accggacgcc accttcgtgc gctatcacac
      361 cgcggcacag cccggcttcc ccgcgctcgc cgtcgacgtc ctgagcagcc cgcaccgcag
      421 cgcgctgacc gacaccgcgg acgtcatctg gtatccgtcg agccgtcccg tggactaccg
      481 cgccgtgacc agccggcccg gcctgcccac cggcagccgg atcgcgtaca gcaacgccga
      541 cgcggccacc gacggcacgc gctccgactg gttcgtcgtc acctgggagt ggcaggcggg
      601 caacgtcttt cagcgcgtca acgtggtcgc cagccagtcg ctgaccggcg accgcctccc
      661 cccggaaccg cagcccgtcg acttccttga cgtgtcgttg cgacccgcac tctgggtggc
      721 gcgccagcaa ccgaacaacc cgggtgacgt cgacgatgtc gtggtcgagc gcgcgatgac
      781 cctggcaggt gtgctgggcg gcaccgccgc cggccgcacg gacggtggcg tggccgatgc
      841 gtgagccgac tccggtcgta ctgccggccc acctgtcggc acgtggactg ctgggccgtc
      901 gcgccctgct gctgctggcc gcggtggtgg ccgtccacgc cgcggccgtg gccaactggc
      961 cgcacatcgt ggcgccggtg ctgaccggga tcttcgcgct cgtctacctg gtgtcgacat
     1021 tggaccggaa ctatctgcta ctcaaggggc ttcactccaa cgcgctggtc agcatcagcg
     1081 ccgaagaggc gctggccatc cccgaccacg agctgccggt gtacaccgtg ctgctgccgg
     1141 tctacgacga accgaccatc gtggccaacc tgatcaacgg cgtcggtcgc ctggactacc
     1201 cggccgacaa gctggagatc ctgctgctcg tcgaggagga cgacctcgcg acccaagagg
     1261 cgctgctggg cgccgacatg cagtcggtgc gcatcgtcat cgtcccgcac agccagccca
     1321 agacgaagcc caaggcctgc aactacggga tgtcactgcc cgacctgcgc ggtgagtacc
     1381 tcaccatcta cgacgccgag gacatccccg acccgcttca gctgcggcgc gcggtcgcgg
     1441 cgttccggcg ggtgccgtcc gacatcggtt gtctgcaggg ccggctcggc tacttcaacg
     1501 agaaccagaa cctgctcacc cggttcttct ccctcgaata cgaccagtgg ttcggggtgg
     1561 tgctgcccgc cgtcgagcag gcccggtgtg tcgttccgct cggcggcacc tccaaccaca
     1621 tgcccaccga ggtgtggcgc gccgtcggcg gatgggacga gttcaacgtc accgaggacg
     1681 ccgacctcgg ggtgcgcctg gcccgcagcg gctaccggac gatgatcctg gactcgatca
     1741 cgttggagga agccaactcc gacgtcgtca actggatccg tcagcggtcc cgctggtaca
     1801 agggctatct gcagaccatg ctcgtgcacc tgcgcgaccc gcggggtctg caccgcgcga
     1861 tcggcacgaa agccttcctg cgaatgctca acatgaccgg tggcgtgccg ctgaccagcg
     1921 cgctcaacgt gctgttctgg ttcaccatgc tgatgtggat cgtcggtaga cccgtcgtca
     1981 tcgggatgct gttccctccg ctcacctact acgtgtgcct gtcactgctg ctgatctgcg
     2041 caccgctgtc ggtgttcacc gggctgatcg tgacccaggc actcggcaaa ccacatctgt
     2101 gggtggccgc cctgctctca ccgctgtact gggtgctgca gtctgtcgcc gccatcaagg
     2161 ccatctacca actcgtattc cgaccgtcgt tctgggagaa gaccattcac gggctctccc
     2221 cgtctcgtga ac
//
LOCUS       DATADN010000649         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1274079, whole genome shotgun sequence.
ACCESSION   DATADN010000649 DATADN010000000
VERSION     DATADN010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274079"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VHH53_08385"
     CDS             complement(<1..615)
                     /locus_tag="VHH53_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2290196.1"
                     /translation="MRHPPRRAAMIPRMGSTAVVIGPGLAVAGGLAVVVAAAIAAAAR
                     LGTGRSTVVAGARAVLQLGVVSLLIGAIVGSVLLSAAFVLVMVGVAAWTSARRITPHR
                     SGWWAVLPIAVAPLPVTALLVVSGVVPPAGIAVIPMAGILIGGAMTATSLAGRRTLDE
                     LRARRGEVEAALSLGFPPRDAAMEIARPSAGQALVPAIDQTRTVG"
     gene            745..2424
                     /locus_tag="VHH53_08390"
     CDS             745..2424
                     /locus_tag="VHH53_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2290197.1"
                     /translation="MTAVAPKPIAPEYPARQSPKGSTFLKMLRTTDPKDIAILYLVTS
                     FGFFLAGGAMALLIRGELAVPGMQFLSTEQYNQLFTMHGTIMLLLYATPILFGFANYI
                     VPLQIGAPDVAFPRLNAFSYWLFLFGGLTVMSGFLTPGGAADFGWFAYTPLSDAIRSP
                     GAGADLWIVGLIVGGLGTILGGVNFITTIVCMRAPGMTMFRMPIFTWNILVTAILILI
                     AFPVLTAALFGLLADRHFGAHVFDPANGGVILWQHLFWFFGHPEVYIVALPFFGIVSE
                     IFPVFSRKPIFGYKTLIYATIAIAALSVAVWAHHMYATGAVLLAFFSFTTFLIAVPTG
                     VKFVNWIGTLWKGQLTFETPMMFAIGFLVTFLFGGITVMGGFITPGGAADFGWFAYTP
                     LSDAIHSPGSGADLWIIGLVVAGLGTILGAVNMITTVVCLRMPGMTMWRMPIFTWNIL
                     LTSVLVLMAFPILTAALLGLMADRHLGAHVFDPANGGVILWQHLFWFFGHPEVYIVAL
                     PFFGIVSEIFPVFSRKPVFGYKSLIYATIAPVAYMWCAHTATESPPMAIVA"
     gene            complement(2409..2465)
                     /locus_tag="VHH53_08395"
                     /pseudo
     CDS             complement(2409..2465)
                     /locus_tag="VHH53_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          341 a          899 c          808 g          422 t
ORIGIN      
        1 cccgacggtg cgcgtctggt cgatcgccgg cacgagcgcc tggcccgcgc tcgggcgggc
       61 gatctccatg gcggcgtcgc ggggcgggaa gcccagcgac agggccgcct cgacctcgcc
      121 ccggcgggcc cgcagctcgt cgagggtgcg ccggccggcc agcgacgtgg cggtcatcgc
      181 gcccccgatg aggatgcctg ccatcgggat caccgcgatc ccggccggag gcaccacgcc
      241 cgagaccacc agcagggccg tgacggggag gggggcaacc gcgatcggca gcacggccca
      301 ccaaccgctg cggtgcgggg tgatgcgccg cgcggaggtc cacgccgcca cgcccaccat
      361 caccagcacg aacgcggcgc tgagcagcac cgagccgacg atcgcgccga tcagcagcga
      421 caccacgccg agctggagga ccgcgcgcgc accagcgacg acggtggagc ggccggtgcc
      481 caggcgcgcc gccgccgcga tggcggccgc cacgaccacc gcgagcccac cggccacggc
      541 caggcccggc ccgatcacca ctgctgtcga ccccatgcgg gggatcatcg cggctctgcg
      601 cggcgggtgt cgcaccgtgc acgacgtgtc gccgggacgt ggatctcgcc acccgggccg
      661 cgccttcgac ggcgggtaga gctaacatcc cgggctgggc cactcggccc gccgctcgtg
      721 cagccggcag ttccaggagg accagtgacc gccgtcgccc ccaagccgat cgcgccggag
      781 tatccagcgc gacagtcgcc caagggctcg accttcctga agatgctgcg gacgacggac
      841 cccaaggaca tcgcgatcct gtacctggtc acgtcgttcg ggttcttcct ggccggcggc
      901 gcgatggcgc tgctgatccg cggcgagctc gccgtgccgg gcatgcagtt cctgtccacc
      961 gagcagtaca accagctgtt caccatgcac ggcacgatca tgctgctgct gtacgcgacg
     1021 ccgatcctgt tcggcttcgc gaactacatc gtgccgctgc agatcggggc ccccgacgtc
     1081 gcgttcccgc ggctcaacgc gttctcctac tggctgttcc tgttcggcgg gctcaccgtg
     1141 atgtccgggt tcctgacgcc gggtggtgct gccgacttcg gctggttcgc ctacaccccg
     1201 ctgtcggacg ccatccgctc gcccggcgcg ggggccgacc tgtggatcgt cggcctgatc
     1261 gtcggtggtc tcggcaccat cctgggtggc gtcaacttca tcaccacgat cgtctgcatg
     1321 cgcgctcccg ggatgacgat gttccggatg ccgatcttca cctggaacat cctggtcacc
     1381 gcgatcctca tcctgatcgc gttcccggtg ctcaccgcgg cgctcttcgg cctgctggcc
     1441 gaccgccact tcggtgccca cgtgttcgac cccgccaacg gcggcgtgat cctctggcag
     1501 cacctgttct ggttcttcgg ccaccccgag gtctacatcg tggcgctgcc gttcttcggc
     1561 atcgtcagcg agatcttccc ggtgttcagc cgcaagccga tcttcggcta caagacgctg
     1621 atctacgcga cgatcgcgat cgccgcgctg tcggtggcgg tgtgggcgca ccacatgtac
     1681 gccaccggcg cggtgctgct ggcgttcttc tccttcacga ccttcctgat cgccgtgccc
     1741 acgggcgtca agttcgtcaa ctggatcggc acgctgtgga agggccagct gaccttcgag
     1801 acgccgatga tgttcgccat cggcttcctg gtcacgttcc tgttcggcgg catcacggtc
     1861 atgggcgggt tcatcacccc cggcggcgct gccgacttcg gctggttcgc ctacaccccg
     1921 ctgtccgacg ccatccactc gccgggctcc ggtgccgacc tgtggatcat cggcctggtg
     1981 gtggccggcc tgggcaccat cctcggcgcg gtcaacatga tcaccacggt cgtctgtctg
     2041 cggatgccag gcatgaccat gtggcgaatg ccgatcttca cctggaacat cctgctgacc
     2101 agcgtgctcg tgctgatggc tttcccgatc ctgacggccg cgctgctggg gctgatggcc
     2161 gaccggcacc tgggcgcgca cgtgttcgac ccggccaacg gtggcgtgat cctgtggcag
     2221 cacctgttct ggttcttcgg ccacccggag gtctacatcg tcgcgctgcc gttcttcggc
     2281 atcgtgtccg agatcttccc ggtgttcagc cgcaagccgg tgttcggcta caagagcctg
     2341 atctacgcga cgatcgcgcc ggtcgcgtac atgtggtgcg cccacacggc caccgagagc
     2401 ccgccgatgg cgatcgtggc gtagaccagg cccttgtagc cgaacaccgg tttgcgggag
     2461 aacaccggga
//
LOCUS       DATADN010000650         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_221166, whole genome shotgun sequence.
ACCESSION   DATADN010000650 DATADN010000000
VERSION     DATADN010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221166"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VHH53_08400"
     CDS             <1..259
                     /locus_tag="VHH53_08400"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX2290198.1"
                     /translation="IRRTETWTNSGCSVHAVVDGAPGTITGTMRLEPEPAGCVLGAEL
                     TAEVPLPLIGGKVERVITDNIGKLMEAEHRFTLQWLRDTGA"
     gene            271..990
                     /locus_tag="VHH53_08405"
     CDS             271..990
                     /locus_tag="VHH53_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX2290199.1"
                     /translation="MELAEYQSRIAAEAALLRAAALSVDADTPVPTCPGWTAQRLVQH
                     VGRVFDMAIRVLQTADPRSPPIWVQPPPGDVFAVLEDRLGLLLDLLSTIDPATPVWHC
                     SPTAPKTAAFWFRRIAHEVTVHRIDARSAAGIDSVVAPDFAADGIDEVLTRLIARHTD
                     TWAVSKLVGTVLYHAADAGRAWAVRLVPGQLPQTVPEAVTEPDASVIGLADALYRAAW
                     GRPSGALISGDRALVDATRAG"
     gene            complement(1069..2007)
                     /locus_tag="VHH53_08410"
     CDS             complement(1069..2007)
                     /locus_tag="VHH53_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX2290200.1"
                     /translation="MGDHGHGHGHGHGAGPSDRRRLGWALAITSVVLLVEVVGTALTG
                     SLALLADAGHLATDVLGLAVALIATVLAQRPPSQMRTYGLGRAEVLAAALNALVLLVV
                     AGVVAVEAVTRLSAPPTVPGVPLLVLGALGLVGNLAALAVLTRGGSGANGSRERSLNL
                     RGAMLEVMGDALGSVAVLGAALVMLTTGWRQADPVASLVIAALIVPRALALLREAAHV
                     LLEGVPRGVDAAEVHRVLCAVPGVRDVHDLHLWTITAGRHAVSAHLVVDEARSLHCGD
                     ASVLDAATLELSQHFGLTHSTLQIEHDDHASHEHTC"
     gene            complement(2057..>2500)
                     /locus_tag="VHH53_08415"
     CDS             complement(2057..>2500)
                     /locus_tag="VHH53_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2290201.1"
                     /translation="RCAVAQIDPALADTAALCEYYEVPLHASANCVVVTGKRAGVQRW
                     AGCVVLATTRADVNGVVRRWLDVRKASFVTRDDVVELTLMEYGGITLLGLPAGWPVLL
                     DPAVVAADQVVVGSGLRRSKLLLPGEVLAALPGAEIVDGLGRPVQ"
BASE COUNT          431 a          901 c          828 g          340 t
ORIGIN      
        1 gatccgccgc accgagacct ggaccaactc cggctgcagc gtgcacgcag tggtcgacgg
       61 ggcgccgggg accatcaccg ggacgatgcg tctggagcca gagcccgccg ggtgcgtgct
      121 gggcgccgag ctcaccgccg aggtcccgtt gccgctgatc ggcggcaagg tggagagagt
      181 catcaccgac aacatcggca agctgatgga agccgagcac cggttcacac ttcagtggct
      241 gcgcgacacc ggggcttagg ctggccaacc gtggagctcg ccgaatatca gtcccggatt
      301 gcggccgagg ccgccctgct acgggccgcc gcgctgtccg tcgacgcgga cactccggtg
      361 ccaacctgcc caggttggac cgcgcaacgg ttggtgcagc acgtcggtcg ggtcttcgac
      421 atggccatca gggtgctcca gaccgctgac ccgaggtcgc caccgatttg ggtgcagcca
      481 ccgccgggcg acgtgttcgc cgttttggaa gaccgattgg gcctcctgct cgacctgctc
      541 tcgaccatcg atcctgccac tccggtctgg cactgctctc ccacggcacc gaagaccgcg
      601 gcgttctggt ttcgccggat cgcgcatgag gtcacggtgc accggatcga tgcgcggtcc
      661 gcggcgggca ttgattcggt ggtagctccg gatttcgccg cggacggcat cgatgaggtg
      721 ctgacccggc tgatcgcgcg gcacaccgac acgtgggccg tctcgaagtt ggtcggcacg
      781 gtgctctacc acgccgcgga cgccggacgg gcctgggctg tgcgcctggt accgggtcag
      841 ctgccccaga ccgtgcccga ggcggtgacc gagccggacg cttcggtgat cgggcttgcc
      901 gacgccctct accgcgccgc ctggggccgc ccatccggtg ccctgatcag cggcgacagg
      961 gccctggtgg acgccacccg ggccggttaa gccgggcagg gatgcccgac acaccgccct
     1021 gcggggcccc gacacaccgg gcatgagcga cagacacgcc gggatgggtt agcaggtgtg
     1081 ttcgtgggag gcgtggtcgt cgtgctcgat ctgcagggtg ctgtgggtga gaccgaaatg
     1141 ctggctgagc tcgagggtgg cggcgtcgag caccgaggcg tccccgcagt gcagcgaccg
     1201 ggcctcatcg accaccaggt gcgcggatac cgcgtgccga cccgctgtga tcgtccacag
     1261 gtggaggtca tgcacatcac gcacacccgg caccgcgcac agcacccggt gtacctctgc
     1321 ggcatcgaca cctcgcggga caccctccag cagcacgtgc gccgcctcgc gaagcagcgc
     1381 cagcgcccga ggcacgatca gcgcggcgat caccagtgag gcaaccgggt cggcctggcg
     1441 ccagccggtg gtcagcatca ccagcgcggc gccgagcacc gccaccgaac cgagagcgtc
     1501 acccatcacc tcgagcatcg cgccgcgcag gttgaggctc cgttcgcggg atccattcgc
     1561 gccggaaccg ccccgggtga gcactgccag cgcggccaga ttgccgacta gaccgagcgc
     1621 cccgagcacc aacaatggaa ccccgggaac ggtcggggga gcgctcaacc gggtgaccgc
     1681 ctccaccgcg accacccccg ctaccacgag taggaccagc gcgttgagcg ccgcggcaag
     1741 aacctcggcg cggcccagcc cgtaagtccg catctgactc ggcgggcgct gggccagcac
     1801 agtggcgatc agcgcaaccg ccagaccgag cacatcagtg gccagatgcc cggcatcagc
     1861 gagcagggcc agcgagccgg tcagggcggt gcccacgact tcgaccagca gcaccacaga
     1921 cgtgatcgcc agcgcccagc cgagccggcg acggtccgat ggtccggcac cgtggccgtg
     1981 tccatggccg tgcccgtggt cgcccatgcg cctcaccata catgcatgct tacgcatgag
     2041 ttaagccgac agaatattac tgaaccggcc gtcccagacc gtcgacgatc tcggcaccgg
     2101 gcagcgcggc cagtacctca cccggcaaca gaagcttgct gcgccgcaga cccgatccca
     2161 ccaccacctg atcggcggca accaccgcag ggtcaagcag taccggccac ccggcaggca
     2221 atcctagcag ggtgatcccg ccatattcca tcaaggtcaa ctccaccacg tcgtcgcgcg
     2281 tgacgaacga cgccttgcgc acatccagcc accgccggac cacaccgttg acgtcagccc
     2341 gggtggtggc caggaccaca catccggccc agcgctgcac accagcacgc ttgccggtga
     2401 ccacgacaca gttggcgctg gcgtgcagcg gcacctcgta gtactcgcac agcgcagcag
     2461 tgtcggccag cgccggatcg atctgggcga cggcacagcg
//
LOCUS       DATADN010000651         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_221314, whole genome shotgun sequence.
ACCESSION   DATADN010000651 DATADN010000000
VERSION     DATADN010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221314"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VHH53_08420"
     CDS             <1..245
                     /locus_tag="VHH53_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX2290202.1"
                     /translation="GTLDELFEALTLVQTKKVTKFPVVLLGRDYWGGLYSWLADTVVA
                     QGKLNEAEFGLVHLTDDIDEAVRLVGISYQAWQDTH"
     gene            365..655
                     /locus_tag="VHH53_08425"
     CDS             365..655
                     /locus_tag="VHH53_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX2290203.1"
                     /translation="MGTVLIYLVVAVVVAAVVFLIASLVFGRGEDLAALPPDATPTWL
                     PEGPIIGDDVRAVRFAQVVRGYRMSDVDWVLHRAAAELDTLHSRVAELEAQQ"
     gene            652..1095
                     /locus_tag="VHH53_08430"
     CDS             652..1095
                     /locus_tag="VHH53_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2290204.1"
                     /translation="MNLTARVDVNAPPETVFAAATDWERQGEWILGTTVRIRHGDGRS
                     VGSEVEAVTGIRGIGVTDRMQITVWEAPARCEVRHLGRVVRGTGIFAVQPRGHGGATF
                     EWTEQLELPLGAVGRLGATLVWPVFGWGLQRSLDRFADFCEDYCQ"
     gene            1110..1691
                     /locus_tag="VHH53_08435"
     CDS             1110..1691
                     /locus_tag="VHH53_08435"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX2290205.1"
                     /translation="MGAVSVPGTDGRLRCPWAITAADYCDYHDTEWGMPVHGEAALFE
                     RLSLEAFQSGLSWLTILRKRTAFRRAFVGFDPAAVAAFTGDDVARLLTDPAIVRNRAK
                     IEATVRNARAVTELGEDLDALLWSYAPDPAGRFTPRTTADVPTRTPESEAMSRTLRRR
                     GFAFVGPITCYALMQATGMVDDHLAGCFRRTAR"
     gene            1854..2021
                     /locus_tag="VHH53_08440"
     CDS             1854..2021
                     /locus_tag="VHH53_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HEX2290206.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVMRVPLEGGGRLVVEMSADEAD
                     ELGEALRAVVE"
     gene            2298..>3139
                     /locus_tag="VHH53_08445"
     CDS             2298..>3139
                     /locus_tag="VHH53_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M17"
                     /protein_id="HEX2290207.1"
                     /translation="MGPGAGTLGVDEQWLAQYRVRATAGSVHVVPLYRTRPDHGWLVG
                     TGRGTAQHWRAAGAALIRASSARVASDAAAGRRPPRIVQVHLPPGTTAHQAAALTLGC
                     VVGSHSYRVSSQARSPGTTTVRLVAPPGQPPQPLISAVARAQLLAQATGLARDLANTP
                     SGHKDPAWLAGTAARLAATVPGLWATVRDERWLAEHGFGGVVAVGGGSARPPRLLELA
                     WRPAEVTTEMTDTDELLVLVGKGITFDTGGISIKPADNMHLMRTDMSGGAAVIAALLA
                     VGQL"
BASE COUNT          463 a         1013 c         1147 g          516 t
ORIGIN      
        1 tcggcaccct cgatgagctg ttcgaggctc tgacgctggt gcagaccaag aaggtcacca
       61 agttccccgt cgtcctgcta ggacgggact actggggtgg gctgtactcg tggctagctg
      121 acaccgtggt ggctcagggc aagctcaacg aggcggagtt cggtctggtg cacctcactg
      181 atgacatcga cgaggcggtg cggctggtcg ggatctccta ccaagcctgg caggacacgc
      241 actgactgca gttccggggc acgacaggtt gggcgccgat ccagcggcgg ttggtagcgg
      301 tttgggacag cggcgagggg ggttggccgt ccttgaagat gcaccaacgt gtcacgatcg
      361 gcatgtgggt acggtgctga tctatcttgt cgtcgccgtg gtggtggcgg ccgtggtgtt
      421 cctgatcgcc tcgctggttt tcggccgggg agaggacctg gcggcactgc cgccggacgc
      481 taccccgacc tggttgccag agggaccgat catcggcgat gacgtgcgcg ccgtgcggtt
      541 cgcccaggta gtacgtggtt accggatgtc tgacgtggat tgggttctgc atcgagcagc
      601 ggccgaactc gacacgctgc actcccgagt tgccgagctg gaggcgcagc agtgaacctg
      661 accgcgcggg tcgacgtgaa tgcgccgccg gaaacggtgt tcgcagccgc cactgactgg
      721 gagcgccagg gtgagtggat tctcggtacc accgtgcgga tccggcacgg tgatggccgc
      781 tcggtcggct cagaggtgga ggcagttacc ggaatacggg ggatcggggt gaccgaccgc
      841 atgcagatca ccgtgtggga ggccccggcc cgctgcgagg tgcggcacct cggtcgcgtg
      901 gtccgcggca ccggcatctt cgcggtgcag ccgcgtggac acggcggtgc cacgtttgag
      961 tggaccgagc agttggagct gccgctgggc gcggtgggca ggctgggcgc gacgctcgtc
     1021 tggccggtct tcgggtgggg cttgcagcgc tcgttggacc gtttcgccga cttctgcgag
     1081 gactactgcc agtgacggcc gagggtccga tgggcgctgt ctcagtgccg ggtaccgacg
     1141 gtcggctgcg ctgcccgtgg gcgatcaccg ccgccgacta ctgcgactac cacgacaccg
     1201 agtgggggat gccggtacat ggcgaggccg cgttgtttga gcggctcagt ctggaggcgt
     1261 tccagtcagg actgtcatgg ttgacgatcc tgcgcaagcg gaccgccttc cgtcgcgcgt
     1321 tcgtgggatt tgaccctgct gcggtcgccg ctttcaccgg tgacgacgtg gcacgcctgc
     1381 tcaccgatcc cgcgatcgtc cgcaaccggg ccaagatcga ggcaacggtc cgcaacgcca
     1441 gggcggtcac cgagctcggt gaggacctcg acgccctgtt gtggtcctac gcgcccgacc
     1501 ccgccggacg gttcaccccg cgtaccaccg ccgacgtgcc gacccgcacc ccggagtccg
     1561 aggcgatgtc gcgcaccctg cgacgtcgtg gtttcgcctt cgttggaccc atcacctgct
     1621 atgcgctcat gcaggccacc gggatggtcg acgaccatct cgccggttgc ttccgccgca
     1681 ccgcacgatg agctccggcg ctggtgagcg cacggtccca ggtggacggt gggctgcgct
     1741 ggcccgagat catcggttgc ttttcacggc tctgggtcga taagggagaa tgcagggggt
     1801 caccggtgtc gcgtggccat cccgcggtga cgaatgcacg gagggagcac gctatggcgg
     1861 ccatgaagcc acggaccggc gacggtcccc tcgaggtgac gaaggaggga cggggcatcg
     1921 tgatgcgcgt ccccctcgag ggcggtggac gtctcgtcgt cgagatgtcg gcggacgagg
     1981 cggacgaact tggcgaggca ttacgagccg tcgtggagtg aaggtacgca tgcagcgtcc
     2041 gtcgtcccgt ggcctcggtc ggcgccggta aggcgcgcgc tggggcctca ccacgaaccg
     2101 atccggagac atcagtgccc gcgacaccga aggtccccac cgccctggcc gaggttcaag
     2161 tccagacgcg gcgtggtccc ggggtgccag atgtcgtcct ggtcggccat ccgccacgtg
     2221 accagccgcc agatggagga tccccagctg ggcattcctc ctctgcacag tccccagcgg
     2281 acgatgaccc accggtcttg ggtcccggcg caggcacgct cggggtggac gagcagtggc
     2341 tggcccagta ccgggtgcgc gccaccgccg gctccgtgca cgtggtgccc ctatatagga
     2401 cgcggcccga tcacgggtgg ctggtgggta ccgggcgtgg cacggcgcag cactggcggg
     2461 cggccggtgc tgcgctgatc cgggccagct cggcgcgagt ggcgagcgat gccgcggccg
     2521 ggcggcggcc cccacgcatc gtgcaggtgc acctgccgcc cggcacgaca gcccaccagg
     2581 cggcggcact gacgctgggc tgcgtggtgg gctcccacag ctaccgagtc agcagccagg
     2641 cccgatcacc agggacgacc accgtgcggc tggtcgcacc acccgggcag ccaccgcaac
     2701 cgctgatcag cgcggtggcc agagcacagc tgctggccca ggccaccggc ctggcccggg
     2761 acctagcgaa caccccgagc ggacacaagg acccggcttg gctcgccggc accgctgccc
     2821 gcttggcggc caccgtgccc ggcctgtggg ccacggtccg tgatgagcgc tggctggccg
     2881 agcacggttt cgggggagtg gttgcggtcg gcggtggctc ggcacgcccg ccgcggttgc
     2941 ttgagctggc ctggcgtcct gctgaggtga ccacagagat gacggacacc gatgagcttc
     3001 tggtgctggt cggcaagggc atcaccttcg ataccggggg aatctcgatc aagccggccg
     3061 acaacatgca cttgatgcgc acggacatgt ccggaggcgc cgcggtgatc gccgcgctgc
     3121 ttgccgtcgg ccagttgag
//
LOCUS       DATADN010000652         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_536892, whole genome shotgun sequence.
ACCESSION   DATADN010000652 DATADN010000000
VERSION     DATADN010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536892"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VHH53_08450"
     CDS             <1..396
                     /locus_tag="VHH53_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX2290208.1"
                     /translation="ITDESDTEQLPPVEHLDDGTVRVISRLPVIDLGELFAVDLEDTD
                     VETVGGLLAQRLGRVPVPGTEAEVAGLRLRAEGGNDRRGRLRITTVIVSRVRSQPDSP
                     ALGEQQQAEQSRLHPVAADPPQEASDTRA"
     gene            389..652
                     /locus_tag="VHH53_08455"
     CDS             389..652
                     /locus_tag="VHH53_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX2290209.1"
                     /translation="MPETADLDPEDAKIVTLARSALARTGADEAAAVRDTEGRTYTGC
                     TVSLPLLALTALQVAVAAAVASGATGLRADQRGAVTDVLRAVG"
     gene            667..1611
                     /gene="era"
                     /locus_tag="VHH53_08460"
     CDS             667..1611
                     /gene="era"
                     /locus_tag="VHH53_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX2290210.1"
                     /translation="MTGDIAGTAARTPFRAGFACLVGRPNVGKSTLTNSLVGHKVAIT
                     SSRPQTTRHTIRGIVHQPDGQLILVDTPGLHRPRTLLGQRLNSLVRATWSEVDVIGFC
                     VPADAAIGPGDRFIAGELAALARRTPVFGIVTKTDLVSPQRVAEQLAGLADLARSAGF
                     DEFAELVPVSAIDGFQVGLLADLLLARMPESPPLYPGGELTDEPEQTLVAELVREAAL
                     EGVREELPHSLAVLVEEMSPREGRADLLDVYVNLYVERDSQKAIVIGRGGQRLKEVGS
                     RARRQIEALLGISVYLDLRVKVAKDWQRDPKQLRRLGF"
     gene            1697..>2226
                     /gene="recO"
                     /locus_tag="VHH53_08465"
     CDS             1697..>2226
                     /gene="recO"
                     /locus_tag="VHH53_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX2290211.1"
                     /translation="MKLYRDTGVVLRVQKLGEADRIVTLLTRRHGRVRAVAKGVRRTS
                     SRFGARLEPFGHVDVQFYRGRTLDIVTQVETVDGFGSRLVGDYGRYTTGCAVLETAER
                     LAGAEGEPVLRLYLLVVGALRALSDGQRQPPLVLDAFLLRAMTAAGWAPALIECARCG
                     APGPHRAFSVPAGGVV"
BASE COUNT          325 a          715 c          822 g          364 t
ORIGIN      
        1 atcaccgacg aatccgacac cgagcagctg ccgccggtcg agcacctcga cgacggtacg
       61 gtgcgggtga tcagccggct gccggtgatc gatttgggtg agctgtttgc ggtggatctg
      121 gaggacaccg atgtggaaac cgtcggtggg ctgcttgcgc agcggctggg ccgggtgccg
      181 gtgcccggta ccgaagccga agtggccggc cttcggctgc gggccgaggg cggtaacgac
      241 cgtcgcgggc ggctgcgcat caccaccgtc atcgtctctc gggtccgctc gcagccggat
      301 tccccagcac tgggcgaaca acagcaggcc gagcaatccc ggctccaccc cgtcgcggct
      361 gacccgccgc aggaagcgag cgatacgcgt gcctgagacc gctgaccttg accctgaaga
      421 cgccaagatc gtgacgctgg cgcgctcggc gctggcccgc accggggccg acgaggcggc
      481 tgcggtgcgc gataccgaag ggcgtaccta caccgggtgc acggtctcgt tacccttgtt
      541 ggcgctcacc gcgctgcagg ttgcggtcgc cgcggcagtg gccagcggcg caaccggcct
      601 gcgggccgac cagcgcggag cagttaccga cgtgctgcgc gcggtgggtt agaagtggcc
      661 cagaatatga ctggcgacat tgcaggaacg gccgcccgga cacctttccg ggccggtttc
      721 gcttgcctgg tcggtcggcc caacgtcggc aagtcgacgt tgaccaactc gttggtgggc
      781 cacaaggtgg cgatcacctc cagccggccg cagaccaccc gccacaccat ccgcggaata
      841 gtgcaccagc ccgacgggca gctcatcctg gtggataccc ccggcctgca ccgcccgcgc
      901 accctgctcg gtcagcggct caacagcctg gtgcgcgcga cgtggtcaga ggtcgacgtg
      961 atcgggtttt gcgtgcccgc cgacgccgcg atcgggcctg gagatcggtt cattgccggt
     1021 gagttggctg cgctggcccg gcgcaccccg gtgttcggca tcgtgaccaa gaccgatctg
     1081 gtgagcccgc agcgggtcgc cgagcagctc gccgggttgg ccgatttggc ccggtcggcc
     1141 ggtttcgacg agttcgccga gctggtgccg gtctccgcga tcgacggttt ccaggtgggg
     1201 ttgctcgccg acctgctgct ggcccggatg ccggagtccc cgccgctgta cccgggtggg
     1261 gagctcaccg acgaaccgga gcagaccttg gtggcagagc tggtgcggga agcggccctg
     1321 gaaggggtgc gcgaggaact accgcactcg ctggccgtct tggtcgagga gatgagccca
     1381 cgggaggggc gggcggacct gctcgatgtc tacgtgaatc tctacgtcga gcgggacagc
     1441 cagaaggcga tcgtgatcgg gcggggtggc cagcggctca aggaggttgg cagccgcgct
     1501 cgccggcaga tcgaggcgtt gctgggcatc tcggtgtacc tggatttgcg agtaaaggtc
     1561 gccaaggact ggcagcgcga ccccaagcag ctacgtcggc tcggtttcta aatcgccgcc
     1621 ctcgtccgcg atatggctgc ttgtcgctgt ccgcgcgccg cgtcgtgcgg cggcatggta
     1681 gtgcgacgat gagcgggtga agctgtaccg cgacaccggg gtggtgctgc gggtacagaa
     1741 gctgggcgag gccgaccgca tcgtcacgct gctgacccgc cggcacgggc gggtgcgcgc
     1801 ggtggccaaa ggtgtgcggc gaacctcatc gaggttcggt gcgcggctgg agccgttcgg
     1861 ccacgtcgat gtgcagttct accgcgggcg gacgctggac atcgtcaccc aggtagagac
     1921 agtcgatggc ttcggttccc ggctggtcgg cgactacggc cgttacacca ccggatgcgc
     1981 agtactggag accgccgaac gactggccgg tgcggagggg gagccggtac tgcggctgta
     2041 cctgctggtc gtaggagcac tgcgcgcgct gtctgatggg cagcggcaac ccccgctggt
     2101 gctcgacgct ttcctgttgc gcgcgatgac cgctgccggt tgggctcccg cgctgatcga
     2161 atgcgcccgc tgcggggcac cgggtccgca ccgggcgttc agcgtgcccg cgggaggtgt
     2221 ggtctg
//
LOCUS       DATADN010000653         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_390329, whole genome shotgun sequence.
ACCESSION   DATADN010000653 DATADN010000000
VERSION     DATADN010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390329"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VHH53_08470"
     CDS             <1..191
                     /locus_tag="VHH53_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290212.1"
                     /translation="WAGGYPDERKAHQQHFVKNLGLLGGLLLAITAGKPAVAKRAKKR
                     ARKLSRKAAFKAAKAKAR"
     gene            383..1480
                     /locus_tag="VHH53_08475"
     CDS             383..1480
                     /locus_tag="VHH53_08475"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HEX2290213.1"
                     /translation="MGHEVRGVVARGKGAPVTVEIVQVPDPGPGEALVAVQACGVCHT
                     DLHYREGGINDEFPFLLGHEAAGVVESVGDGVDTVAPGDFVILNWRAVCGRCRACRRG
                     KLWYCFNTHNAAQRMTLSDGTALSPALGIGAFAEKTLVHSGQCTKVNPAVAAEVAGLL
                     GCGVMAGLGAVLNTGGVTRGDSGAVIGCGGVGDAAIAGAVLAGATTIIGVDVDPGKLR
                     WATEFGATHTINARESDVVETVREYTGGFGADVVIDAVGRPETWEQALFARDLAGTVV
                     LVGVPTPDLMVPSIPLLEFFGRGGALKSSWYGDCLPERDFPMLVDLHLQGRLPLEKFV
                     SETISLDDVEHAFEKMERGEVLRSVVLLSSS"
     gene            1503..>1852
                     /locus_tag="VHH53_08480"
     CDS             1503..>1852
                     /locus_tag="VHH53_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2290214.1"
                     /translation="MIDRVVTSGTFSLDGETWEVDNNVWLIGDDREVIIVDAAHDAEA
                     IAAAVGDRQVLGIVCTHAHDDHVSAAPDLARKLVAPVLLHPAEHVLWRLAHPDTPPPN
                     AELADGDVLRVAGTA"
BASE COUNT          306 a          591 c          660 g          295 t
ORIGIN      
        1 tctgggccgg tggctaccct gatgagcgta aggcccacca acagcacttc gtgaagaact
       61 taggcctgct cggtggtcta ttactggcga tcacagccgg caagcccgct gtggccaagc
      121 gcgccaagaa gcgcgccaga aagctttcgc gcaaggcagc cttcaaggca gccaaggcca
      181 aggcacgctg agcgcaagct caactggcta gccaaggacc ccgtttcggc cgacattgac
      241 ggaacggggt tcgttcatgc ccagatactg tccgcgtccc atcgcgagtc atgcacccgt
      301 cgtggcaggc tggccgccgt cggtgtcacc gagcacggtg ccatgcagtc ccggtgtcgc
      361 gagataatgc ggggaggtca cgatgggcca cgaagtacgt ggagtggtag cgcgcggtaa
      421 gggcgcgccg gtaacggtgg agatagtgca ggtgcccgac ccgggccccg gcgaggcact
      481 ggtcgctgtc caggcatgcg gggtgtgcca caccgacctg cactaccgcg agggcgggat
      541 caacgacgag ttccctttcc tgctcgggca tgaggccgcc ggcgtcgtgg agtccgtcgg
      601 tgacggcgtc gacaccgtgg cacccggcga cttcgtcatc ctcaactggc gcgcggtgtg
      661 cggtcgctgc cgcgcgtgcc gacggggcaa gctctggtac tgcttcaaca cccataacgc
      721 cgcccagcga atgacgctgt ccgacggcac cgctctgtcg cccgcgctgg gcatcggtgc
      781 gttcgccgag aaaacactgg tccacagcgg ccagtgcacc aaggtcaatc ccgcggtcgc
      841 ggcggaagtg gccgggttgc tcggctgcgg tgtcatggcc gggctcggcg cggtgctcaa
      901 caccggcggc gtcacccgcg gtgactcggg cgcggtgatc ggctgcggag gggtaggcga
      961 cgccgccatc gccggggccg tgctggcggg cgcgacaacg atcatcggcg tcgacgtgga
     1021 cccgggcaag ctgcggtggg ccaccgagtt cggcgccacg cacacgatca acgcccgcga
     1081 gtccgacgtc gtcgagacgg tacgggaata caccgggggc ttcggagccg acgtcgtgat
     1141 tgacgcggtg ggccggccag agacctggga gcaggcgctc tttgcccgtg acctggctgg
     1201 cacggtggtg ttggtcggag tgcccacccc ggacctgatg gtgcccagca tcccgctgct
     1261 ggagttcttc ggccgtggcg gggcgctgaa gtcgtcgtgg tacggcgact gcctgcccga
     1321 gcgggacttc ccgatgctcg tcgacctgca tctgcaggga cggctaccgc tggagaagtt
     1381 cgtttccgag acgatcagcc tggatgatgt ggagcacgcc ttcgagaaga tggagcgcgg
     1441 cgaggtgtta cgcagcgtcg tgctgctgag cagctcgtga gcgcgaccgg agggagcgcg
     1501 cagtgatcga tcgggtggtc acgtcgggca ccttcagcct ggacggcgag acctgggagg
     1561 tggacaacaa cgtttggctg atcggtgacg accgtgaagt gatcatcgtc gacgccgcac
     1621 acgacgcgga agcgatcgcc gctgcggtgg gcgaccggca ggtgttgggc atcgtgtgca
     1681 ctcacgccca tgacgatcat gtcagcgccg caccggacct ggcccgcaag ctcgtcgcgc
     1741 cggtgctgtt gcacccagcg gagcacgtgc tgtggcgcct cgctcatccc gataccccac
     1801 caccgaacgc cgaacttgcc gacggcgacg tactacgcgt cgcagggacc gc
//
LOCUS       DATADN010000654         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_993366, whole genome shotgun sequence.
ACCESSION   DATADN010000654 DATADN010000000
VERSION     DATADN010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993366"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VHH53_08485"
     CDS             <1..1128
                     /locus_tag="VHH53_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290215.1"
                     /translation="DNAVNADTTRGTYQQTSVTLTSTLTEDQGLLVEIVDSGPGLPPD
                     QLQAINDRLASPPADDASVSRQMGLFVVSRLAAQHGITVRLRQRLSQIGISTAVSLPS
                     SVVSIDSRPSHGARPSNDRIPEAPATTEWSGTGAPLPLQVSVIDEAAAAELFSPTSIG
                     IAPSPPGGPRTAEQEWRELFGGDHTQPQPESQREPLAGQLDVDQQGGAAPDPLASPLP
                     LSSAAATGQPQEVREEIFEMVSAWFRERQSASATNGDTTEMAVPPNGVRSANAHEWRS
                     PLDEEWQAAQALATPVDHGVTMAGLPKRQPLAHLVSGAEGIGRSVAASMPVGPVRVPD
                     DVRGRLSRYQHGLRVGRHARIGPDEQLTMTDTMHGHIEEDQ"
     gene            1128..1562
                     /locus_tag="VHH53_08490"
     CDS             1128..1562
                     /locus_tag="VHH53_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEX2290216.1"
                     /translation="MTTAVPQHPVQPSQFGWLVDSFVERVPGVAHAVVVSADGLLLVA
                     SSGLPRDRADQLAAVTSGLVSLTQGAARCFEAGGVVQTVVEMERGIVLTMVISDGSSL
                     AVLASPSCDIGLIGYEMTLLVDRAGKLLTPELRAQLQSAART"
     gene            complement(1567..>2373)
                     /locus_tag="VHH53_08495"
     CDS             complement(1567..>2373)
                     /locus_tag="VHH53_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX2290217.1"
                     /translation="RDIGLVADVESAGGATVYRVTEDTVRRAMDVGRSATDLHELFRT
                     RSRTPVPQSLSYLIDDVARRHGRLRGGTAAAFLRCDDAVLLAELLAHPVAARCGLRRI
                     APTVLVSPLALGPLLEEMRGAGFAPIAEGADGQVVTLRAAERRARVRPSAMRRSAVPA
                     VPGVPSSQQLAALIRGVRAGDAAANTPRGPALVTAGTSTGNTLELLQGAADAGSNVWI
                     GYVDSHGVASKRIVRPVSVGGGVLEGFDRSRGELRRFPLHRITSAAVTQD"
BASE COUNT          391 a          820 c          805 g          357 t
ORIGIN      
        1 gacaacgccg tcaatgccga taccaccagg ggtacgtacc agcagacctc ggtgaccctc
       61 accagcacgc tcaccgagga ccagggtctg ctggtggaga ttgtcgactc gggccctggc
      121 ctgccgccgg accaactaca agcgatcaac gaccggctcg cctcgccacc ggcggacgat
      181 gcgtcggtct cccggcagat gggcctgttc gtggtaagca ggctggctgc ccagcatggc
      241 atcacagtgc ggctgcgcca gcgcctcagc caaatcggga tcagcacggc ggtgtcgctg
      301 ccgtcctctg tggtgtcgat cgattcccga ccttcccacg gcgcccggcc ctcgaatgac
      361 aggatccccg aggcgccggc caccaccgag tggagcggga ccggcgcgcc gctgccactg
      421 caggtgtctg tgatcgacga agccgccgcg gcagagctgt tcagcccgac atcgatcggg
      481 atagcaccat caccgcccgg cggcccgcgc acggctgaac aggagtggcg cgagctcttc
      541 ggtggcgacc atacccagcc ccagcccgag tctcagcgcg agccgcttgc cggtcaactg
      601 gatgtggatc aacaaggtgg ggcggcgcca gatcccctgg cgagtccgct gccgttgagc
      661 agcgccgcgg caaccggtca gccgcaggaa gtccgcgagg agatcttcga gatggtctcg
      721 gcctggttcc gcgagcggca gtccgcgtca gcaaccaacg gtgacaccac ggagatggcc
      781 gtaccgccga acggggttcg gagcgccaat gcacacgagt ggcggtcccc gttggacgag
      841 gagtggcagg cggcgcaagc gttggctacc ccggtcgacc acggcgtcac catggccggg
      901 ctacccaagc gccagccgct tgcgcatctt gtgtccggag cggagggcat cggccgttcc
      961 gttgcggcgt cgatgcctgt tgggccggtc cgcgttccgg atgatgtgcg cggccgactg
     1021 agcagatacc agcacggatt gcgcgtcggg cgtcacgccc gcatcggtcc tgacgaacag
     1081 ctcaccatga ccgacaccat gcacggacat atcgaggagg atcagtagtg acgacagcag
     1141 tgccacagca tccggtccag cccagccagt tcggctggct ggtggacagc ttcgtagagc
     1201 gcgtacccgg tgtggcgcac gctgtcgtcg tctccgccga cgggctgctc ctcgttgcgt
     1261 catcgggatt gccccgggac cgtgccgatc agctcgccgc ggtcacttct ggtttggtga
     1321 gcctcaccca aggcgcggcg cgctgcttcg aggcaggagg cgtggtccag accgtggtgg
     1381 agatggagcg cggcatcgtc ttgaccatgg tcatcagcga tggctccagc ttggccgtgc
     1441 tggcctcgcc aagctgcgat atcggtctga tcgggtatga gatgaccctg ctggtcgatc
     1501 gggcaggcaa gctgctgacc cccgagctac gtgcccaact gcagagcgct gcgcgcacgt
     1561 gaggggtcag tcctgagtga ccgccgcaga ggtgatccgg tgtagcggga agcgccgcag
     1621 ctcgccgcgg gaccggtcga acccctccaa tacgccgcct cctacgctca ccggccgcac
     1681 gatccgcttg cttgctacgc catgggagtc cacgtagccg atccacacat tgctgccggc
     1741 gtcggcggcg ccctgcagca gctccagtgt gttgccggtg gacgtcccag cggtcaccaa
     1801 cgcaggtccg cgcggggtgt tagctgcggc gtccccagcc cggacgccgc ggatcagcgc
     1861 ggcaagctgc tgcgaagaag gcacaccggg cacggcaggg acggcgctcc ggcgcatcgc
     1921 ggacggtcga accctggccc gacgctccgc ggcgcgcaac gtgaccacct ggccgtcggc
     1981 cccctcagcg atcggtgcga agccggcgcc acgcatttcc tcgagcaggg ggcccagtgc
     2041 caatgggctg accagcacgg tgggagctat cctgcgcaac ccgcagcgcg cggcgaccgg
     2101 atgcgccagt aattcagcca gcagcacagc gtcgtcgcaa cgcaggaatg cggccgcggt
     2161 gcctccgcgc agccgcccgt gccgtcgagc cacatcgtcg atgaggtagc tcagcgactg
     2221 cggcaccgga gtccgggacc gggtccgaaa cagctcgtgc aagtcggtgg cgctacggcc
     2281 gacgtccatg gctcgacgca cggtgtcctc ggtgacccgg tagacggtgg cgccgccggc
     2341 cgactccacg tcggcgacca gcccgatatc cct
//
LOCUS       DATADN010000655         4319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_221723, whole genome shotgun sequence.
ACCESSION   DATADN010000655 DATADN010000000
VERSION     DATADN010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221723"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1629)
                     /locus_tag="VHH53_08500"
     CDS             complement(58..1629)
                     /locus_tag="VHH53_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX2290218.1"
                     /translation="MAELSGVELEVFRHALAGVAEEMGVALRRAAYSPNIKERADCSA
                     AVFDPDGQMVAQAEHIPVHLGAMPASVRAVLDTYGALRPGQQVCVNDPYLGGTHLPDL
                     TIVAAVGAAGGSQRDTVLLGYVANRAHHADVGGAAPGSMPATATEIAMEGVRIPPILI
                     ADAGGERDDVVRLIAANSRTPRERRGDLRAQFAANHVGALRLRELANRMGRSRLYEAM
                     AAVCDYSDRRVRIAVAEIPDGCYRNSDVLEIGAGVTIRAAVTVAGDEVSIDFEGTDAQ
                     IPVNLNAVFAVTLSSAMFVFRMLTDPDAPPNAGCYRSLHVRAPEGSVVHATFPAPTAA
                     GNVETSQRIVDVLLGCFAQAIPDRVPAASQGTMNNLLIGSGGDHPFSYYETLGGGEGG
                     TPGRPGMSGIHTGMTNTRNTPAEAMELDYPLRVWRYELRSHSGGAGAHPGGDGLIREV
                     EVLADCTLTVQSERREHPPWGLRGGYPGAVGRNVLRRADGTEEELPGKGTWQLRRGDR
                     VRIETPGGGGWGSPA"
     gene            complement(1622..3607)
                     /locus_tag="VHH53_08505"
     CDS             complement(1622..3607)
                     /locus_tag="VHH53_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX2290219.1"
                     /translation="MAQHIGACAGVDVGGTFTDVVVHAAGQRPRAVKVPTTPDNQAVG
                     VERGVTQSRAETAPSQHMAQLRLLPHGTTTATNAVLERKIARTVLVTTDGFADVLQIA
                     RQDRPALYDLTVTRPEPLVPHDRVVTVIERTSPDGEVVIALTGEEIERVVRRVECLKP
                     ESVAISLLFSYAGDEHERRLAEALARLQVPITRSSALLPEFREFERASTCVLNAAIEP
                     VMRRYLSNLTDRLPGPTITVMTSGGGTAGVDFVAGAPVHTLLSGPAAGVVAAGAVARG
                     AGFADAIALDMGGTSTDVCLIRAGQPEVSTSAQIAGLPFRTPAVGIHTVGAGGGSIAW
                     VDAGGALRVGPGSAGAEPGPACYGLGGTEPTVTDAHCVLGHLDPDRPLGGGLRLDINA
                     AADAIAALPESAGAAPGILAVVRATMARALRKVSTERGVDPAGLALVAYGGAGPLHAT
                     SLARELRCRAVVVPPAPGVLSALGLLLAPARYEASRTVMAPVEEDLTGAWAALEDQAR
                     QELHRQGVTGEITVSRVADARYAGQSHELRITAAADADLAELLHRAHQQAYGYAMPEE
                     RVLVVTLRVVARGEPVLSQPPGDWDQGQPAPERSREIGIGDQTVKARVVSRAALATGD
                     EVSGPALIEQSDTTTLLDADEVGVLDDAGNLVVHLHG"
     gene            3787..>4319
                     /locus_tag="VHH53_08510"
     CDS             3787..>4319
                     /locus_tag="VHH53_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX2290220.1"
                     /translation="MSIRSITLLGLVSLVAAFTAAGQGSATQTGCTRTITRADEVHAA
                     LAAASPGNILCFSGADLAGAALMMTRSGTADAPIRLVSDGSTVREVQIIADYVLIQGF
                     TIAGGGELLLSGTGIIAQKNTVRDTQRSGIVCTSCIDSTIESNAVHHAAATGISIGGQ
                     RITVRSNVVSATVACAEG"
BASE COUNT          669 a         1562 c         1417 g          671 t
ORIGIN      
        1 gtcgacgcga cgacgacgtg atcaggcggc atggaataaa tggtacgagg ccgttcgcta
       61 tgccgggctc ccccaacccc cgccgcccgg tgtttcgatg cgcacccggt cgccgcggcg
      121 cagctgccag gtgcctttgc cgggcagttc ttcctcggtc ccgtcagccc ggcgcaagac
      181 gttacgaccc actgcgccgg gatagccacc acgcaggccc cacggcgggt gttcccggcg
      241 ttcggactgc acggtcagcg tgcagtcggc cagcacctcg acctccctga tcagcccgtc
      301 cccaccggga tgcgcccccg caccacctga gtgcgaccgc agctcgtagc gccacacccg
      361 cagcgggtag tccagctcca tcgcttcggc gggggtgttt cgcgtgttgg tcatcccggt
      421 gtggatcccg gacatgccag gacggccggg cgtgccgccc tcgccgccgc ccagcgtctc
      481 gtaatagctg aacggatgat ccccaccgga accgatgagc aggttgttca tcgtgccctg
      541 gctggccgcc ggtacccggt ccggaatcgc ctgagcgaag cagccgagca ggacgtcgac
      601 gattcgctga ctggtctcga cgttgcccgc ggccgtcggt gcgggaaacg tcgcgtgcac
      661 gacggagcct tccggtgcac gcacgtgcag cgatcggtaa caaccggcat tgggcggtgc
      721 gtcggggtcg gtcagcatcc ggaacacgaa catcgccgac gacagcgtca cggcaaagac
      781 cgcgttgagg ttcaccggga tctgagcatc ggtgccctcg aagtcgatcg acacctcatc
      841 gccggcgacg gtcacagcgg cgcggatggt cactccggct ccgatctcca ggacgtcgga
      901 gttgcggtag cacccgtccg ggatctccgc caccgcgatc cggacccgcc ggtcggagta
      961 gtcgcacacg gctgccatcg cctcgtacag ccgggacctc cccatccggt tggcgagttc
     1021 gcgtagccgc agggctccga cgtggttggc cgcgaactgg gcccgtaggt caccccggcg
     1081 ctccctgggc gtccgcgagt tcgcggcgat cagccgtacc acgtcgtcac gttccccgcc
     1141 ggcgtcggcg atcaggatgg gcggtatccg gacgccctcc atggcgatct cggtagcggt
     1201 ggcgggcatg gaccccggtg ctgctccgcc gacgtcggcg tggtgtgcac gattggccac
     1261 atagccgagc aacacagtgt cgcgctggga gccgccagca gctccgacgg cggcgacgat
     1321 ggtgaggtcc ggcaggtggg ttccaccaag gtacgggtcg ttgacgcaga cctgctgccc
     1381 aggtcgcagc gcaccgtagg tgtcgagcac cgcgcggacg ctggccggca tggcgcccag
     1441 gtgcaccggg atgtgctcag cttgggcgac catctgcccg tcggggtcga acaccgcggc
     1501 cgagcagtca gctcgttcct tgatgttcgg cgagtacgcc gcgcgccgca gggccacacc
     1561 catctcctct gcgaccccgg ccagcgcgtg acggaacacc tcaagctcga cgccagacaa
     1621 ctcagccatg caggtgcacc accaggttgc cggcgtcatc gagcacgccg acctcgtcgg
     1681 cgtcgagcaa tgtggtggtg tcggactgct cgatcaacgc tgggccgctg acctcgtccc
     1741 ctgtggccag cgccgctcgg gagaccactc gggccttgac ggtctggtca ccgataccga
     1801 tttcgcgaga ccgctccgga gccggctggc cttgatccca gtcccccggt ggctgcgaca
     1861 ggaccggctc accgcgcgcc accacccgca gcgtgacgac cagcacccgc tcctcaggca
     1921 tcgcgtagcc gtatgcctgc tggtgtgcgc ggtggagcag ctcggccaga tccgcatcag
     1981 ccgcagcggt gatccgcagc tcgtgggact ggccggcata tcgagcatcg gcaacccggg
     2041 acacggtgat ctcacccgtc acgccctggc ggtgcagctc ctggcgggcc tgatcctcca
     2101 gcgccgccca ggcaccggtg agatcctcct cgaccggcgc catcaccgtc cgggacgcct
     2161 cgtagcgagc cggcgccagc agcagcccca gcgcgctgag cactccgggt gccggcggca
     2221 ccaccaccgc acgacagcgc agctcgcggg ccagcgaggt cgcgtgcagc gggccggcac
     2281 caccgtaagc gaccagcgcc aagccggccg ggtcgacgcc acgctcggtg ctgaccttgc
     2341 gcagcgcgcg agccatcgtg gcccgcacca ccgccaggat cccgggcgct gccccggcgg
     2401 actccggtag cgcagcgata gcgtccgcgg cagcgttgat atccagtcgc agacctccgc
     2461 cgagtgggcg atccggatcg aggtggccca gcacgcaatg tgcatcggtc accgtcggct
     2521 cggtgccacc cagcccgtag caggctgggc caggctcggc accggccgat cctggaccga
     2581 cccgcagggc gcctccggcg tcgacccagg cgatcgatcc accgccggcg cccaccgtgt
     2641 ggatcccgac cgccggggtg cgaaacggta acccggcgat ctgcgccgac gttgacacct
     2701 cgggctggcc cgccctgatc aggcagacgt cggtggacgt gccgcccatg tccagggcga
     2761 tcgcgtcggc gaaaccggcg ccacgcgcca ccgcacccgc ggccaccaca cccgcggccg
     2821 gcccggacag caacgtgtgc accggggccc cggccacaaa gtcaacgccg gcggtgccgc
     2881 cgcccgaggt catcacagta atcgtcggcc cggggagccg gtcggtcagg ttggacaggt
     2941 agcgacgcat caccggctcg atcgcggcat tgagcacgca cgtcgacgcc cgctcgaact
     3001 cgcggaactc cggcagcaac gcgctggatc gggtgatcgg cacctggagc ctggccaagg
     3061 cttcggccag ccgccgctcg tgctcgtcgc cggcgtagct gaacagcagg ctgatcgcca
     3121 ccgactccgg cttcaggcat tccacccggc gcacgactcg ctcgatctcc tcgccggtca
     3181 gcgcgatgac cacctcgcca tccgggctgg tccgctcgat cacggtcacc acccggtcat
     3241 gcggcaccag cggttcgggg cgagtcaccg tcaggtcata gagcgcgggc cggtcctgcc
     3301 gggcgatctg cagcacgtcg gcgaacccgt cggtggtcac caggacagtc cgggcgatct
     3361 tgcgttccag gaccgcattg gtggccgtcg tggtgccgtg cggcaggagt cgcaactgcg
     3421 ccatgtgctg gctgggcgca gtctcagccc ggctttgtgt cactcctcgc tcgacgccca
     3481 cggcctgatt gtccggggtg gtcggtacct tcaccgccct gggccgctga ccggcggcgt
     3541 gcacgacgac atcggtgaac gtgcccccga catcgacccc agcgcaggcg ccgatgtgtt
     3601 gcgccatccc tgaccttctc ctctcgagtc tgtcgggtcg gtaaggattc atcctgcacg
     3661 tccaactcgt gaatcggcgc gcctgtacgc taagtgacaa ttgcgtctac ggagcgttac
     3721 tctcgccacg atgaaagtcg ctgtcttggt actggggcgg tatcggtgac gagagtggct
     3781 cgctcggtgt cgatccggtc tatcacgctg ctcgggctgg tcagcctcgt cgctgctttc
     3841 accgccgcgg gccagggcag cgcgacgcag accggctgca cccgcacgat tacccgagcc
     3901 gacgaggtac atgctgcgct agctgccgct tcaccaggaa atatcctgtg tttcagcggt
     3961 gctgacctcg ctggtgcagc cctgatgatg accagatcgg gtaccgccga cgcgccgatc
     4021 cgtctcgtct ctgacggcag cacggtccgc gaagtccaga tcatcgccga ctacgtcctc
     4081 attcaaggct tcacgatcgc cggcggtggg gaactgctcc tgtcgggaac cgggatcatc
     4141 gcgcagaaga acacggtccg cgatactcaa cgaagcggga tcgtctgcac ttcctgtatc
     4201 gactccacca tcgagtccaa cgccgtgcac catgccgccg ccaccggcat ctccatcggc
     4261 ggccagcgaa tcaccgttcg gtccaacgtc gtgagcgcga cggtggcctg tgccgaggg
//
LOCUS       DATADN010000656         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_684451, whole genome shotgun sequence.
ACCESSION   DATADN010000656 DATADN010000000
VERSION     DATADN010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684451"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1203..>1563
                     /locus_tag="VHH53_08515"
     CDS             1203..>1563
                     /locus_tag="VHH53_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290221.1"
                     /translation="MTEATQFDPDRLRVALDHGEVVELDGGTLVLDGEESPSVVLRLA
                     PWRARSVAQALEEWSAASGMFNRTSRPSPDELDLSRTLELAASVLGDGDEPLLRFPHG
                     DYTVPSRQRLAAVAVLAE"
BASE COUNT          288 a          462 c          540 g          273 t
ORIGIN      
        1 actgatcagt ctatgacgac tacgagaaag ttaccgtgtg gagcaaagac cgctacggac
       61 gccggggctg gatcattcag gaaacgacca gtactgcctg cagcagcttt atgcggcccg
      121 ccgcccaccg cacagtacct atcacgcgct gggacaggtt gacccgacgc gccctcggtg
      181 ggtgtacaac caggaatcga ccgagctgcg tacggaggtt ggcaacgctt ggcatgccgg
      241 ctactggtgg tttcagtggc gcccagcggc agcatgtccg cacggatggc cttgaagccg
      301 cactgcgcat cggggaatcg ggtacgcagg gtgacttgca caagagcgtt ggggcagcgc
      361 ctgcgtggtc ttccccgtcg tcggctccgg caagcgcctc ttcgcggacg gcgccgccac
      421 ctcggcgtac acactgctcg acgcgaagtc accagcaccg gcgtgattca tcaggtcctg
      481 cggccgaccg tgtttggacg gccgagttca tggtcgagga cggccacgaa gccgtccggc
      541 agccgagtag gcgcagtccg gctggccacc ggcgaatgac agcggacaga tcaagttggg
      601 tcgggcggtg tgggacaccg acggtgtcgc cgccgacgag cgtgactacg ttgccgacac
      661 ctcggcgagc ccgacagggt gctggtcacc gacgagacgg gatctgaaga ttgctcgtgc
      721 acggtgggcg ccgtcatgac gctgatgggc tggtcgcaca cctcgatggc ggcggtatga
      781 gcacatcacc gagacgatcc gatactgcgg aagcggaggg attcgaaccc ccggtccctc
      841 gcgggacgct cgctttcaag gtctgcgaag cgacgtacgg gagcagccgg ggtgtgtgtt
      901 accgctgatc gcggttaaga accaccgtag cccggaccgt ggtgaacgga cacgaatgag
      961 acgagaaatg agacgagcct tgcagcgtcg accaagatcg tcaaaatcct gttgcagtct
     1021 tcacgagccc gaggactagg catggagcgc cctgactgat tagcggattg acctggtgtt
     1081 gcgctggtcg agccagtgtg atccatggtg ccagctcggc tattgagtag gcggtcaggg
     1141 caagtgtttg cgccacctgc cctgaccttg atcccaaccc taagatggag ggtggagacc
     1201 cgatgacaga ggctacgcag ttcgatcccg atcgacttcg ggtggctcta gatcatggtg
     1261 aggtcgtgga actcgacggc ggcacgctcg ttctggacgg cgaggagtcc ccgtcggtgg
     1321 tgctgcggtt ggcaccatgg cgggctcggt cggtggcgca ggcattggaa gagtggtcgg
     1381 ctgcctccgg gatgttcaac cggacgagcc gaccaagccc tgacgagctg gacctgtctc
     1441 gcaccctaga gctcgcggcg tccgtgctcg gcgacggcga tgagccactc ctgcggtttc
     1501 ctcacggcga ctacacggtg ccgagtcggc agcgattggc ggcggtagcg gtgctggccg
     1561 agc
//
LOCUS       DATADN010000657         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1443892, whole genome shotgun sequence.
ACCESSION   DATADN010000657 DATADN010000000
VERSION     DATADN010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443892"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VHH53_08520"
     CDS             complement(<1..198)
                     /locus_tag="VHH53_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290222.1"
                     /translation="MTDRLATLPSESKAYRTSGASTLGIDEAQEGSESGATRRPFCAL
                     DEASYHLDSISEPWSVHLEVRL"
     gene            complement(236..877)
                     /gene="folE"
                     /locus_tag="VHH53_08525"
     CDS             complement(236..877)
                     /gene="folE"
                     /locus_tag="VHH53_08525"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX2290223.1"
                     /translation="MSTVDPARPLTERPASVGHRSLRVVLPRREVDLPAAERAVADLL
                     VALGKDPASEHLSATPRRVAHSYAELLTPREFDLTTFPNDEGYDELVLAKSIPVQSLC
                     EHHLLPFHGIAHIGYLPGGRILGLSKLARVVDLFARDLQVQERLTQQVADGLQEHLAP
                     KGVGVVIEAEHLCMSLRGVRATGSRTVTSALHGLLREDARSRQEFFALTGVAS"
     gene            complement(998..1432)
                     /locus_tag="VHH53_08530"
     CDS             complement(998..1432)
                     /locus_tag="VHH53_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290224.1"
                     /translation="MITAGVSTSSTARKAVLSDPAYQAFRLLRTVFTVAPILFGLDKF
                     TNLLVDWPSYLAPWIDGIVPGTAQQAMYAVGVIEIVAGIAVALAPRFGAWLVAAWLAG
                     IIVNLLTFPGYFDIALRDFGLLVGAVALTRLATPYASGPRKL"
BASE COUNT          266 a          506 c          579 g          254 t
ORIGIN      
        1 gaggcggact tccaggtgga cgctccacgg ctcggagatc gagtcgaggt gatagctggc
       61 ctcgtcgagc gcgcagaagg gtcgccgcgt tgctccggac tccgagcctt cctgcgcctc
      121 atcgattccc agggtgctgg cgcccgatgt gcggtacgcc ttgctctcgg atggcagagt
      181 ggccaggcgg tctgtcatga cgttgctcct cttgcggcct tgcggacgac ctcggtcagg
      241 acgccacgcc ggtgagcgcg aagaactcct gccgggagcg ggcgtcctca cgcagtagcc
      301 cgtgcagtgc tgaggtcacg gtgcgcgagc cggtggctcg gacgccgcgc agtgacatgc
      361 acaggtgctc cgcctcgatc actacgccga cgcctttggg cgccaggtgc tcctgcagcc
      421 cgtcggcgac ctgctgggtc aggcgctcct gcacttgcag gtctcgagcg aacaggtcga
      481 cgacgcgcgc gagcttggac agcccgagga tgcgcccacc gggcaggtaa ccgatgtggg
      541 cgatcccgtg gaagggcagc agatggtgct cgcacagcga ttgcacgggg atgctcttgg
      601 cgagcaccag ctcgtcgtag ccctcatcgt tggggaaggt cgtgaggtcg aactcccgcg
      661 gggtgagcag ctcggcgtag gagtgggcga ctcgtcgcgg tgtggcggac aggtgttccg
      721 aagccggatc cttacccagg gccaccagca gatcggccac tgcgcgctcc gccgcgggca
      781 ggtccacctc cctccgcggc agaactacgc gcaggctgcg gtgaccaacg gatgctggtc
      841 gctcggtcag cggtctagcg ggatcgacgg tgctcatcgg ctggtccttt ggtgaggggt
      901 cggggagggc ttgaggcggc ggctcgcccc caagccctcc gggagtctgc gttcgaggtg
      961 gggcaaaaca cagaccggtc ccggcgcgat cacgccgtca gagcttccgc ggtccggatg
     1021 cgtagggcgt ggccagccgg gtcagcgcga ccgcgccgac gagcaacccg aaatcacgca
     1081 gggcgatgtc gaagtagccg gggaaggtca acaggttgac gatgatgccg gctaaccagg
     1141 ccgccaccag ccaggcaccg aaccgcggcg ctagcgcaac cgcgatcccg gccacgatct
     1201 cgatcacgcc tacggcgtac atcgcctgct gcgcggtgcc gggcacgatg ccatcgatcc
     1261 acggggccag gtagctcggc cagtccacga gcaggttagt gaacttatcc agcccgaaaa
     1321 ggatcggcgc cacggtgaac accgtgcgca gcagccggaa cgcctgatat gcggggtcgg
     1381 acaggaccgc tttgcgagcg gtgctcgacg tcgagacacc tgcggtaatc atgggaatct
     1441 ccttctcaag atttcggtca aggacctgaa tggctatgcc accggaatgc cgtgggcagc
     1501 cggctggcca tgtggttgaa gtgggacagg ccggacaggc gcggcgatag tgcgcacttc
     1561 ggcggtcgcc ggggtcagcg cccgcagggt ggccatcgtg ggggg
//
LOCUS       DATADN010000658         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_222176, whole genome shotgun sequence.
ACCESSION   DATADN010000658 DATADN010000000
VERSION     DATADN010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222176"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..442)
                     /locus_tag="VHH53_08535"
                     /pseudo
     CDS             complement(1..442)
                     /locus_tag="VHH53_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948622.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            complement(478..948)
                     /gene="rpsG"
                     /locus_tag="VHH53_08540"
     CDS             complement(478..948)
                     /gene="rpsG"
                     /locus_tag="VHH53_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX2290225.1"
                     /translation="MPRKGPPPKRPLISDPVYGSPLVTQLVNKVLVEGKRSVAERIVY
                     HALEGCRDKTGTDPVVTLKRALDNVKPAIEVKSRRVGGATYQVPVEVRAVRSTTLALR
                     WLITFSRQRREKTMVERLMNELLDASNGLGASVKRREDTHKMAESNKAFAHYRW"
     gene            complement(948..1322)
                     /gene="rpsL"
                     /locus_tag="VHH53_08545"
     CDS             complement(948..1322)
                     /gene="rpsL"
                     /locus_tag="VHH53_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX2290226.1"
                     /translation="MPTIQQLVRKGREDKVAKTKTAALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTSQVEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYKIIRGSL
                     DTQGVKGRKQARSRYGAKKEKS"
     gene            1888..>2215
                     /locus_tag="VHH53_08550"
     CDS             1888..>2215
                     /locus_tag="VHH53_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2290227.1"
                     /translation="MPGPLAGLRVIELAGIGPGPHAAMLLADLGADVVRVERPPGGLQ
                     LAPRDSDQMLRGRRSVALDLKDPADRETVLRLVERADVLIEGLRAGVTERLGVGPADC
                     LSRNPRL"
BASE COUNT          324 a          728 c          723 g          440 t
ORIGIN      
        1 agtagaagtc cgcgcccagc ttgtccatct tgttgacgaa gcagatgcgg ggcacgtcgt
       61 actagtcggc ttgccgccac accgtctcgg actggggctc gacaccctcc ttgccgtcga
      121 acaccgcgac cgcaccgtcg agcacccgca gcgaccgctc cacctcgacg gtgaagtcga
      181 cgtggcctgg cgtgtcgatg atgttgatct ggtggtcgtt ccagaaggtg gtggtggcag
      241 cagaggtgat cgtgatgcca cgcttctgct cttcctccat ccagtccatc gtggcggcgc
      301 cgtcgtgcac ctcaccgatc ttgtagttga tcccggtgta gaacaggacc cgctcggtgg
      361 tggtggtctt gcccgcgtcg atgtgcgcca tgatcccgat gtttcggacc ctggcgaggt
      421 cagtcagcac ttcacgtgcc acgagagttc atccctagct gtctcgaaag tggttcgtca
      481 ccagcggtag tgcgcgaagg ccttgttcga ctcggccatc ttgtgggtgt cctcacggcg
      541 cttcacgctc gccccgaggc cgttgctggc gtctagcagt tcgttcatca ggcgctcgac
      601 catggtcttc tcccgccgct gccgggaaaa cgtgatcagc cagcgaagtg cgagcgtcgt
      661 ggagcgcacc gcacgcactt cgaccggaac ctggtaggtg gcgccaccga cgcggcggct
      721 cttcacctcg atggctggct tgacgttgtc cagcgcgcgc ttgagagtga ccaccgggtc
      781 ggtgccggtc ttgtcccggc agccctccaa cgcgtggtac acgatcctct cggccaccga
      841 gcgtttgccc tcgacgagga ccttgttcac cagctgggtc accagcggcg agccgtagac
      901 cgggtcggag atgagcggtc gcttcggggg cggacctttg cgaggcatca gctcttctcc
      961 ttcttcgcac cgtagcgact gcgggcctgc ttacgaccct tgacgccctg agtgtccagc
     1021 gatccgcgga tgatcttgta ccgcacgccg ggaaggtcct tcactcgacc gccgcgcacc
     1081 aagacgatgg agtgctcctg gaggttgtga ccctcaccgg gtatgtaagc cgtcacctcg
     1141 acctgactgg tgagccggac gcgggcgacc ttgcgcagcg ccgaattcgg cttcttcggc
     1201 gtcgtcgtgt agacgcgggt acacaccccg cgtcgctgcg ggctgccctt cagtgccgca
     1261 gtcttggtct ttgcgacctt gtcctcgcgg cccttgcgga ccagctgctg gatggtaggc
     1321 atggaccagc tttcttccgt cttctggcgg tggtgctctg tcatctactg cgcgtctggc
     1381 tactgttcat ctggttacta ctcgtctggc gctgtcgtgc ttccggtcgc cggttgggcg
     1441 ttgcgcggcc ggtcactccg tatcccgagg tcgggcgtgt cgctgttggg cccagccgat
     1501 tgtcagaatc ggtcccgact ggagctcggc ggcaccctcg ccaggccgga gatggcggat
     1561 cctgagtaac tggacccgca ccgccggtgg tgcaccgcca acaggcacgc ggagctgccc
     1621 gaccggagtc gggcaccggg accaggatac cgggggcatt catgaagggt caaaacgccc
     1681 ccttcctgcc tgcttcggat cggccgtcgc gaccggactc gacgcagtgc ccagcgttat
     1741 ctactctgca gtaactatgg atttcgcagg catcaaggtg ctcaccggtc accggcggcg
     1801 cggcccgcac tagacatcac aacacctgct gtaagcgctg cattccatgc cggcgacagc
     1861 ccgcacggcg gaacgaggag gagtgcgatg ccgggaccgc tggccgggct gcgggtgatc
     1921 gagctggccg ggatcggtcc tggtccacat gcggcgatgt tgttggcgga tctgggcgct
     1981 gacgtcgtcc gagtggaacg tccgccaggt ggcctgcagc tcgccccgcg cgacagcgac
     2041 cagatgcttc gcggccgccg ctcggtggcg ctcgacctca aggaccccgc cgaccgggaa
     2101 accgtgctgc gcctggtgga gcgagccgac gtgttgatcg aagggctgcg cgccggtgtc
     2161 accgagcggt tgggcgtcgg tccggccgac tgcctgtccc gcaacccacg tctgg
//
LOCUS       DATADN010000659         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_222240, whole genome shotgun sequence.
ACCESSION   DATADN010000659 DATADN010000000
VERSION     DATADN010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222240"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1874
                     /locus_tag="VHH53_08555"
     CDS             225..1874
                     /locus_tag="VHH53_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX2290228.1"
                     /translation="MPIQPIPPETARIARAAFPKGNRYLRAADELDSLFTDEAFLALF
                     PTHGQPALPPWRLALVTLLQFAEGLSDRQAAHAVRSRIDWKYVLRLELTDPGFDASVL
                     SEFRARLITSAAEFLLFETLLTWCRDRQLIKARGRQRTDATHILAAVRALNRIEVVGE
                     TMRHALNTLAVVAPEWLRAVSPPDWQARYARRAEDDRLPSTQAARAALALTIGHDGWQ
                     LLAAVDHPDAPPWLREVPAVVILRRVWLQNYWWDGTQLRWREADNIPPAGQFISSPYD
                     AEAHYARKYTTQWVGYKVHLTETCEDDLPHLITHIETTPGPTADGATTPKIHAALQQR
                     GLLPGTHIVDTGFLDADLLVQSRDDYGVDLLGPTRLDYHWQAQAGAGFDAQHFQIDWD
                     RQHATCPAGKTSISWTPAVDNRGNAVIKVKFSTRDCRPCPKLALCVRSQKRYPRRTIT
                     IRPERQYQALQAARQREATDAFRAEYARRAGIEGTISRGTRSARLRRTRYIGLARVHL
                     GHILTAAGLNLLRLGEWFLETARAKTRLMPITRLMADAPAA"
BASE COUNT          397 a          724 c          636 g          372 t
ORIGIN      
        1 tcggcacccc gctcaccttc accgtgctgg acgatcgcgg caacacggtg acgaccattt
       61 ccacacagtc caccggcacc gtggggacgg acatcctccg gctgcgggac gccttcaaca
      121 acagtctttt gatactgctg gcgaagtcct gaaagcactg gtacgttgcc tcggcatcgt
      181 gacagtttcg cctatagacg tggaggtaca ccatgcttcg cccgatgccg atccaaccca
      241 ttccgccgga aacggcccgt atcgcccgcg ccgcctttcc caagggcaat cggtacctac
      301 gagcagccga tgaactggat agcctgttta ctgatgaggc ctttctggcg ctcttcccca
      361 cgcacggaca acccgcgctg cccccctggc ggctggcgct cgtcaccctc ctccagttcg
      421 ccgaaggcct ctccgatcgc caagcagctc acgcggtgcg cagccgcatc gactggaaat
      481 atgtgctgcg cttggaactc acggatcctg gattcgacgc ctcagtcctc agtgaatttc
      541 gcgcccgcct catcaccagt gcggcggagt tcctgctctt cgagacattg ctcacgtggt
      601 gccgcgaccg ccagctgatc aaggcgaggg gccggcagcg gacggatgcc acccacattc
      661 tggccgcggt gcgagccctg aaccggatcg aagtggtcgg tgaaacgatg cggcatgccc
      721 tgaatacgct tgcggtcgtc gcgccggagt ggttacgcgc cgtgagccct cctgactggc
      781 aggcccgtta tgcacgacgg gctgaggatg accgcctccc gagcacgcaa gccgcccggg
      841 cagccctggc gctgacgatc gggcacgatg gctggcagtt gctcgccgcc gtcgatcatc
      901 ccgacgcacc accctggctg cgcgaggtgc ccgcggtcgt gatcctccgc cgggtctggc
      961 tccaaaatta ttggtgggac ggcacccagc tgcgctggcg cgaagcggac aacatcccgc
     1021 cggcggggca gttcatcagc tctccctacg acgctgaagc tcactatgct cgcaagtaca
     1081 ccacgcagtg ggtcggctac aaggtgcatc tcaccgaaac ctgtgaggat gatctgccgc
     1141 acctgatcac gcacatcgaa accacacccg gcccgaccgc cgacggggcg acgaccccca
     1201 aaatccacgc cgccctgcag cagcggggcc tgctgcccgg cacccatatc gtggacaccg
     1261 gcttcctgga cgccgatctc ctcgtccaga gtcgggatga ctatggggtg gacctgctgg
     1321 ggcccacccg cctcgactat cactggcaag cccaagccgg agctggcttt gatgcccagc
     1381 atttccagat tgattgggat cggcagcacg cgacctgtcc agccggcaag accagtatca
     1441 gctggacgcc cgccgtggat aaccgcggca atgccgtcat caaggtgaag ttttcaacca
     1501 gggactgtcg gccatgtcct aagttagcgt tatgtgtgcg ctcgcagaag cgctacccgc
     1561 ggcggacgat caccatccgg cctgagcggc aatatcaagc cctgcaggcg gcccggcaac
     1621 gggaagcgac cgacgcgttt cgggcggagt atgcgcgccg tgctggcatc gaagggacga
     1681 tctcgcgcgg gacacggagc gcgcgcctgc gccgcacccg ttatatcggc ctcgcgcggg
     1741 tgcatctggg gcacatctta acggccgcgg gactgaacct gctgcggctt ggggagtggt
     1801 tcctggagac cgcgcgtgcc aaaacccgcc tcatgccgat cacacggctg atggccgatg
     1861 cccccgcagc gtagcccgcg aagacttcgc cagcagtagg tcatgagttc cgctcaccac
     1921 cgcggatgaa aatggttata gtacatagca ccgccgtggc gtgaagaagg tcgagagatt
     1981 ctagtgccat cagcctgcca acagtaggac ctgggtgctc tgtggcacca ccgtctgtcg
     2041 ctcctccttg ggggaagcac gcagcagaga atctgcatga aaagggcacc ccatgcacgg
     2101 ccatctcctc acccatgttc atcctttgg
//
LOCUS       DATADN010000660         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_66176, whole genome shotgun sequence.
ACCESSION   DATADN010000660 DATADN010000000
VERSION     DATADN010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66176"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VHH53_08560"
     CDS             complement(<1..760)
                     /locus_tag="VHH53_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2290229.1"
                     /translation="MVEPRRIVILGSGLAGATAAGELRDKGFSGEVVVIGKEQHRPYE
                     LPALSKGILLGEAEEPDWVHDADFYPKHDIDLRLSTVATELHLGDRVVVDENGTRHQY
                     DRLLLATGSRPRMLPVPGADLDGVRTLRTLDDARALLAALLANPGRAVIVGAGWIGCE
                     VAAAARKHGVDVTVVERLALPLHQVLGDTIGAVFRDLHTQHGVQWRFGVDLAEITGES
                     GRVRGVRLSDGTDLPADLVVVAVGAVPRVELAHAA"
     gene            939..1136
                     /locus_tag="VHH53_08565"
     CDS             939..1136
                     /locus_tag="VHH53_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290230.1"
                     /translation="MSGQREWSVSCRDIAGRRRAVQVIASHGQVVLVVPPGETAILSP
                     LEVGRLRAALRDAVVVAAEED"
     gene            complement(1153..>1527)
                     /locus_tag="VHH53_08570"
     CDS             complement(1153..>1527)
                     /locus_tag="VHH53_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX2290231.1"
                     /translation="WTQMSFSAAQDNFHAAARAGIDARVYWPGIGEVPVSELVLRKLL
                     PMAHEGLDAWGVATDDRDRLLGVIEQRCITGRNGASWQVDTVRRLEDTTQVNREQALR
                     EMTRRYASHMHSNEPVHTWPAV"
BASE COUNT          223 a          576 c          492 g          236 t
ORIGIN      
        1 cggccgcgtg cgccagctcc accctcggca ccgcacccac cgcgaccacc acgaggtctg
       61 cgggcaggtc cgtcccgtcg ctcagccgca ccccgcgtac ccggcccgac tcgccggtga
      121 tctccgccag gtcgacgccg aagcgccact gcaccccgtg ctgggtgtgc aggtcgcgga
      181 acaccgcgcc gatcgtgtca ccaagcacct ggtgcagcgg caacgccaag cgctcgacca
      241 cggtcacgtc gacgccatgc ttgcgcgcgg ccgcggccac ctcacacccg atccagccgg
      301 ccccgacgat caccgcccgg cccgggttgg ccagcagcgc cgccagcaac gcccgggcgt
      361 cgtcgagcgt ccgcagcgtg cgcaccccgt cgaggtcggc accgggcacc gggagcatgc
      421 gcggccgcga gccggtcgcc agcagcagcc ggtcgtactg gtggcgcgtg ccgttctcgt
      481 cgaccaccac ccgatcaccg aggtgcagct cggtcgccac cgtcgacagc cgcaggtcga
      541 tgtcgtgttt ggggtagaag tccgcatcgt gcacccagtc cggctcctcg gcctcgccga
      601 gcaggatccc cttggacagc gcgggaagct cgtatggccg gtgctgttcc ttgccgatca
      661 ccaccacctc acccgagaat cccttgtctc gcagctcacc ggcggcggtt gcgccggcca
      721 gcccagatcc gaggatgaca atgcgacgtg gttcgaccat gatcagtctc cggtttgcct
      781 cgaacgccgg acatgtccaa ccggacgcta gtaccccgcg atcacacgcg cctcaacggg
      841 caccgaaagt cgacgatctg gcgtcacgac gactcaggaa tcccgtccac ccgttcatcg
      901 acacgtccgg cttgggaacg cgcgggaggt agttcgctgt gagtggccag agggaatggt
      961 cggtctcctg ccgagacatc gccggccggc ggcgcgccgt gcaggtgatc gcctcgcatg
     1021 gtcaggtcgt gctggtcgtt ccgcccgggg agacggcgat cttatccccg ctcgaagtcg
     1081 gccggctgcg tgccgcgctc cgcgacgccg tggttgtcgc cgccgaggag gattgaccgc
     1141 tcggggccgt agctagacgg ccggccaggt gtgtactggc tcgttgctgt gcatgtgcga
     1201 cgcgtaccgc ctggtcatct cccgcaaggc ctgctcgcgg ttcacctgtg tggtgtcctc
     1261 caaccggcgc accgtgtcca cctgccagct cgccccgtta cggccggtga tgcaccgctg
     1321 ctcgatcacg ccgagcagcc ggtcccggtc gtcggtggcc acgccccagg cgtccagccc
     1381 ctcgtgcgcc atcggcaaca gcttgcgcag caccaactcg gacaccggaa cctcgccgat
     1441 gccgggccag tacacccggg cgtcgatgcc ggccctggcc gcggcgtgga agttgtcctg
     1501 tgcggccgag aagctcatct gtgtcca
//
LOCUS       DATADN010000661         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_66453, whole genome shotgun sequence.
ACCESSION   DATADN010000661 DATADN010000000
VERSION     DATADN010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66453"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VHH53_08575"
     CDS             complement(<1..1181)
                     /locus_tag="VHH53_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2290232.1"
                     /translation="MSDRQERLRTYRAKRDFSVTAEPLGSELAAGSGRFVVQRHRARR
                     LHYDLRLELDGVLVSWAVPRGPTLDPKAKQLAVHVEDHPLEYFDFEGVIPHGEYGGGD
                     VIVWDWGTWQPSKADDPVRAIERGELHFDLQGEKLAGRFVLVRTDKDRPGKEQWLLLH
                     KNDDHARPGWNPEDHPKSVKTGRTNDEVAAAPEALWRGDLPADQAAVRLDPVSGPTWD
                     PPSEEELAALDALESQGRWAVQGRELKLTNLNKVLFPSQEGQHPITKRDLVRYHALIA
                     PFMLPYLAGRPVNSHRYPDGVDRPGFWHKEVPSHAPKWLTRWRNEEADPGETQWYAVI
                     DSVPALVWLANFGVVELHPWTSRLPDVHQPTWALIDIDPGSASTFDDVLVLARLYRVA
                     L"
     gene            complement(1194..>1606)
                     /locus_tag="VHH53_08580"
     CDS             complement(1194..>1606)
                     /locus_tag="VHH53_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX2290233.1"
                     /translation="LDIVPQQSPARGKELDMAVGLIESMSGPWRPDDYHDTYSERVEQ
                     LIEDKRHGRELVTAAQPPAATEMSDLLAALQRSVDSARGGGSRPDRQDGNERGLAEAS
                     KAELTEMARDLDVPGRSKMDRAELEKAVAEASGD"
BASE COUNT          241 a          563 c          520 g          282 t
ORIGIN      
        1 tccagcgcca cccggtacag ccgcgcaagc accaggacgt cgtcgaaggt gctggccgag
       61 ccgggatcga tgtcgatcag ggcccaggtc ggctggtgca cgtcgggcag ccgagacgtc
      121 cacgggtgca actccaccac gccgaaattc gccagccaga cgagagcggg cacgctgtcg
      181 atgacggcat accactgggt ctcccccggg tcggcctcct cgttgcgcca gcgggtcagc
      241 cacttcggtg cgtgactggg aacctccttg tgccagaagc cagggcggtc gacgccatcg
      301 gggtatcggt gcgagttcac cgggcggcca gccaggtagg gcagcatgaa aggcgcgatc
      361 aaggcgtggt agcgcaccag gtcgcgcttg gtgatcgggt gctgcccttc ctgggagggg
      421 aagagcacct tgttcaagtt ggtcagcttg agttcacggc cctgcacagc ccaccggccc
      481 tgggattcga gtgcgtccag cgcagcgagc tcttcctcac tcggtggatc ccaggtaggt
      541 cctgatactg gatctaaccg gaccgcggcc tggtcggcag gcaggtcacc gcgccacagc
      601 gcctccggag ctgctgcgac ctcgtcgttg gtgcgcccgg tcttgaccga tttggggtgg
      661 tcttcagggt tccacccggg ccgcgcgtgg tcgtcgttct tgtgcagcaa cagccactgt
      721 tctttgcccg gccggtcctt gtccgtgcgg acgagcacga aacgaccggc gagcttctcg
      781 ccctggagat cgaagtgcag ttcgccgcgc tcgatcgctc gcaccgggtc atcagccttc
      841 gatggctgcc acgtccccca gtcccacacg atgacgtcgc caccgccgta ctccccgtgc
      901 ggaatgacgc cctcgaagtc gaaatattcg aggggatggt cctcaacatg gaccgccagt
      961 tgcttagcct tcgggtcaag cgtcggcccc ctgggcacgg cccagctgac cagcaccccg
     1021 tccagttcca gccgcaagtc atagtgcagg cggcgggcgc ggtggcgttg caccacgaac
     1081 cgccccgaac ccgccgccag ctccgacccg aggggctctg cggtcaccga gaaatcccgc
     1141 ttggcccggt acgtccgcag ccgctcctgc cggtcgctca tcgcacgctg cggtcagtca
     1201 cccgacgcct cggccacggc cttctccagc tcggcacggt ccatcttcga ccggcccggg
     1261 acgtcgaggt cgcgggccat ctcggtgagt tcggccttgc tggcctccgc cagaccccgc
     1321 tcattgccgt cttggcggtc cggccggctc ccgccgccgc gggcggagtc gacgctgcgc
     1381 tgcaaggccg ccagcaaatc ggacatctcg gtggccgcag gcggttgcgc cgcggtgacc
     1441 agctcgcggc cgtggcgctt gtcttcgatg agctgctcca cgcgttcgct gtaggtgtcg
     1501 tggtagtcat ccgggcgcca aggcccactc atcgactcaa tcaatccaac cgccatgtcc
     1561 agctccttgc cgcgagctgg tgactgttgc ggcacgatgt ccaggt
//
LOCUS       DATADN010000662         2019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_66659, whole genome shotgun sequence.
ACCESSION   DATADN010000662 DATADN010000000
VERSION     DATADN010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66659"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VHH53_08585"
     CDS             complement(<1..1974)
                     /locus_tag="VHH53_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX2290234.1"
                     /translation="MATALFDISFNFQEFDGGLYGAINYNTDLFDAATIERMAQRLQL
                     LLAGIVEGPDRPLGQLPWMSEAERHQVLHVWNDTGQLMPVGTVASLFAEQVGRTPEAV
                     AVVSDEVSLSYAELQARANRLANRLVRLGVQPECPVGLLMERSVDVVVAVLAIVKAGG
                     AYLPVDVRAPVERMRLVLAEAGASVLITDRAWEQVAREIHGEQFVVVDADTSWNEESA
                     EAPVVEVWPDSLVYAEYTSGSTGVPKGVAVRHRDVAALAFDRRFTGEAHRRVLVHSPL
                     AFDASTYELWVPLLNGGQVVVAPPSDLDVDTLRRMVTEHQVTALWLTSGLFRMVAQES
                     PECLFGVGEVWTGGDVVPAGAVRRVLRACPGLVVVDGYGPTETTTFATSYRMSDVESV
                     PELVPIGAPLDNMRVYVLDADLRPVPVGVPGELFIAGAGLARGYLNRSGLTAQRFVAC
                     PFGVSGSRMYRTGDWVRWSAQGQLEYLGRTDEQVKIRGFRIELGEVESVLAGHPEVGQ
                     VAVLAREDQPDVKRLVAYVVPATGEVLDSAGLRAHLASRLPDYMVPSAFVTLDGLPLT
                     PNGKLDRKALPAPDFTAAGVGYVAPRTDAETVLAGIWADVLRVEQVGVNDNFFELGGD
                     SILSIQVVSRVRVAFGAEVSPRSLFTNPTVAGLA"
BASE COUNT          464 a          773 c          488 g          294 t
ORIGIN      
        1 cgccaaaccc gccaccgtcg gattggtgaa caacgaacgc ggtgacacct ccgccccaaa
       61 agccacccgc acccgcgaca ccacctgaat actcagaatc gagtcgccac cgagctcgaa
      121 gaaattgtcg ttgaccccga cttgttctac acgcagtaca tcggcccaaa tcccagccaa
      181 cacagtttcg gcgtcggttc gtggcgctac ataacccaca cccgctgcgg tgaagtccgg
      241 tgccggtaat gccttacggt ctagtttccc gttcggggtc aacggcaacc cgtccagtgt
      301 cacaaacgct gagggcacca tgtaatccgg caaccgagac gccagatgcg cccgcaaccc
      361 agccgaatcc agcacctcac cggttgccgg cacgacatag gccaccagcc gcttcacatc
      421 cggctgatcc tcccgagcca acaccgcaac ctgaccaacc tcaggatgcc cagcaagaac
      481 agactccacc tcacccaact caatccggaa cccacgaatc ttcacctgct catcagtccg
      541 acccagatac tccaactggc cctgagccga ccaccgcacc cagtccccgg tgcgatacat
      601 ccgcgaaccc gacacaccaa acgggcacgc cacaaaccgc tgcgcagtca accccgaacg
      661 gttcaaatac ccacgagcca gacccgcccc ggcgatgaac aactcacccg gaacacccac
      721 cggaaccgga cgcagatccg cgtcaagcac atagacccgc atgttgtcca acggggcacc
      781 gatgggcacc agctcgggca ccgactcgac atcggacatc cggtatgaag tggcgaacgt
      841 ggtggtctcg gtgggcccgt aaccatcgac cactaccagg cccgggcagg cccgcagcac
      901 ccggcgcacc gcaccggcgg gtaccacgtc tccgccggtc catacctcgc ccaccccaaa
      961 aagacactcc ggtgattcct gagccaccat ccggaacaat ccggaggtca accacagcgc
     1021 cgtcacctga tgctcggtga ccatgcgccg cagcgtgtcc acatccaggt cgctcggtgg
     1081 ggccaccacc acctggcctc cgttgagcaa cggcacccac aactcataag tcgaggcgtc
     1141 aaatgccaac ggtgagtgca ccaacacccg ccggtgagcc tccccggtga aacgccggtc
     1201 aaaggccaat gccgccacgt cgcgatgccg taccgccacg cccttaggca caccagtcga
     1261 tcccgaggtg tactcggcgt aaaccaggct gtccggccag acttccacca cgggtgcctc
     1321 agccgattcc tcattccacg acgtatcggc gtccaccacg acaaactgtt caccatggat
     1381 ttctcgggcg acctgctccc aggctcggtc ggtgatcagc accgacgcgc ccgcctcggc
     1441 caacaccaac cgcatacgct ccaccggtgc acgcacgtcc accggcaaat acgccccgcc
     1501 tgccttgacg atcgccaaca ccgccacaac aacatcgacc gagcgctcca tcaacaaccc
     1561 gactgggcat tccggctgga caccaaggcg cactaaccga ttcgccaatc gattcgcccg
     1621 cgcctgcaac tcggcataac tcagcgacac ctcatcactg accaccgcga cggcttccgg
     1681 agtacgcccc acctgctcgg cgaacaaact cgctaccgtc cccaccggca tcaactgacc
     1741 ggtgtcattc cacacgtgta gcacctgatg gcgctcggcc tcagacatcc acggcaactg
     1801 ccccaacggc cgatccggac cctcaacaat cccggccaac aacaattgca accgctgggc
     1861 catccgctcg atcgtagccg catcgaatag gtcggtgttg tagttgatgg cgccatacaa
     1921 gccaccatcg aattcctgga agttgaagct gatgtcgaaa agcgcggtgg ccatcggtgg
     1981 ctggacgcct tcgatagcca accccggtag tgtccaggc
//
LOCUS       DATADN010000663         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_66672, whole genome shotgun sequence.
ACCESSION   DATADN010000663 DATADN010000000
VERSION     DATADN010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66672"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VHH53_08590"
     CDS             <1..598
                     /locus_tag="VHH53_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290235.1"
                     /translation="CLTCVAANLLYVLGITDIPDTRCVDRELGREPGSAAQRAAARRF
                     LLQQGLCLHMVCAYEPERFLQEGLNYLRRYYRQEWDSSWDEYWTRHRLEQHRCECLAT
                     QELSIFGARMRTEHRVPTLADIRGALDRGRLVWISVDNNWADVDCHAVLVYGQRGNVF
                     DIYSPEVARSCLQQYHRRRLDRVWLRSEGMTAMWPPEP"
     gene            complement(603..1559)
                     /gene="ligD"
                     /locus_tag="VHH53_08595"
     CDS             complement(603..1559)
                     /gene="ligD"
                     /locus_tag="VHH53_08595"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2290236.1"
                     /translation="MSRASAAESRAGVGLTNLDQPLFDGADATKRDLVNYLDAVRDGI
                     LGALRDRPLSVIRVRPGQQPFMQKNVPKYTPDWVPTTTVWAEASRREVAYALCNDRRT
                     LLWFANQRAVEYHPTLVPDGASHPTHLVLDLDPPAGAAFRHVVRAAQLVRQALADAGL
                     AGAVKTSGVKGLHVFVPLDNRVGIEEAAAATRALAARAERLAPDAVTTAFIREDRDGK
                     VFLDSTRSSGATVVAAYSPRARPGVPVSFPVTWDELDRVVPGDFTIRTAAALLADGDP
                     WHERMPGPQLLPADLVEQGRAIPIARVQAMHEGKRRARVRRQ"
BASE COUNT          245 a          530 c          545 g          257 t
ORIGIN      
        1 atgcttgacg tgtgtcgctg cgaacctact gtacgtgtta gggatcaccg atatccccga
       61 cacccggtgt gttgaccgcg agcttggtcg ggagccgggc agcgcagcgc agcgcgccgc
      121 ggccaggcgt ttcttgctcc agcaggggct ctgcctgcat atggtgtgtg cctacgagcc
      181 tgagcggttc ctccaggagg gcctcaatta cctgaggcgc tactaccgcc aggagtggga
      241 ctcgtcgtgg gacgagtact ggacacggca tcggctggaa cagcaccgct gcgagtgtct
      301 ggcgacccag gagttgagca tcttcggcgc ccggatgcgc actgagcacc gggtacccac
      361 gctcgccgat atccgcggcg ccctggaccg cggccgcctg gtgtggattt ctgtcgacaa
      421 caactgggcc gacgtcgact gtcacgcggt tctggtatac ggccagcggg gcaacgtgtt
      481 cgacatctac tcgcccgagg tcgcacgtag ctgcctgcag cagtaccacc gtaggcgact
      541 ggacagggtg tggctgcgca gcgagggcat gaccgcgatg tggccgccag agccatgatg
      601 tgctactgcc ggcggacgcg ggcgcgccgc tttccctcgt gcatcgcctg tacccgggca
      661 atggggatgg cgcggccctg ctcgaccagg tccgcgggca gcagttgcgg cccgggcatc
      721 cgttcatgcc acgggtcacc gtcggctaat agcgcggcag cagtgcggat ggtgaagtcg
      781 cccgggacaa cgcggtcgag ctcgtcccat gtcacgggga aggacacggg tacgccggga
      841 cgggcccgcg ggctgtaggc cgccaccacc gtggcaccgc tagagcgggt ggagtccagg
      901 aacaccttgc cgtcgcggtc ctcccggata aatgccgtcg ttacggcgtc gggagcgagc
      961 cgctcggccc gcgcggcaag ggccctggtc gccgccgccg cttcctcgat ccccacccgg
     1021 ttgtcgagcg gcacgaacac gtgcaggccc ttcaccccgc tggtcttgac cgcgcccgcc
     1081 aatccagcgt cggccagggc ttgccgtacg agctgggctg cccgcaccac atgccggaac
     1141 gcggcaccag ccggtggatc gagatcgagc accaggtgcg tcggatggct cgcgccgtca
     1201 ggtaccaacg tcgggtggta ttccacagcg cgctggttgg caaaccacaa cagcgtgcgc
     1261 cggtcattgc acagcgcgta ggccacttcg cgtcgggacg cctcggccca tacggtggtg
     1321 gtcggcaccc agtccggagt gtacttgggc acgtttttct gcatgaacgg ctgctggcct
     1381 ggccgcacgc ggatcactga cagcggccgg tcccgaaggg cgcccaggat gccgtcccgg
     1441 acagcgtcca ggtaattcac caaatcccgc ttggtggcgt ccgcgccgtc gaacagcggt
     1501 tggtcgaggt tggtcaggcc gaccccggca cgggactccg cggcgctggc acggctcacc
     1561 cgcgccgccg gccaagc
//
LOCUS       DATADN010000664         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_994847, whole genome shotgun sequence.
ACCESSION   DATADN010000664 DATADN010000000
VERSION     DATADN010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994847"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..807)
                     /locus_tag="VHH53_08600"
     CDS             complement(43..807)
                     /locus_tag="VHH53_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2290237.1"
                     /translation="MSPGTPCWVELSVPDLAAAKEFYGAVFGWSFFDSGEEFGHYQIC
                     QVGGRAAAAIGPCQSESQPTVWTVYVASDDADGTAKLIGENGGTVLVEPFDIPGNGRM
                     CIAQDPVGAAFGVWQAAGTNGAQVYGEPGSLVWTDVRLPDPDAGRTFYASVFGYRYEP
                     IDGEQPDYTTIHFDAARDGEPVGGIGGMMGAPEGTPPHWLTYFLVPDADAAAASASAA
                     GGSILAEPFDTPYGRMAGITDPHGATFFVVGTMPST"
     gene            complement(1402..1677)
                     /locus_tag="VHH53_08605"
     CDS             complement(1402..1677)
                     /locus_tag="VHH53_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX2290238.1"
                     /translation="MTVTGIFTGIIVGLIIGVLARLVLPGKQAIPIWLTIVIGAIGAL
                     LGTLIAQYLGVAVTRGIDWIEIVMQVAIAAVGIALAAGAYGRRRVNR"
BASE COUNT          335 a          627 c          612 g          317 t
ORIGIN      
        1 gccagggcgg cgactgcgtc cgggcccgcc cacagctcat gatcacgtcg atggcatcgt
       61 gcctacgacg aagaaggttg ctccgtgcgg gtcggtgatc cctgccatcc gtccgtacgg
      121 cgtgtcgaac ggctcggcca gaatgctgcc gccagccgcg ctggcgctgg ccgccgccgc
      181 gtcggcgtcg ggcaccagga agtaggtcag ccagtgcggc ggggtgccct ccggggcgcc
      241 catcatcccg ccgatgccgc cgaccggctc gccgtccctg gccgcgtcga agtggatagt
      301 cgtgtagtcg ggctgctcgc catcgatggg ttcgtagcgg tagccgaaga ctgaggcgta
      361 gaacgtgcgg ccggcgtctg gatcgggcag ccgcacatcg gtccagacca acgatccagg
      421 ctcgccgtag acctgggcgc cattcgtccc ggcggcctgc catacgccga aggccgcgcc
      481 caccgggtcc tgcgctatgc acatccggcc gttgccggga atgtcgaacg gctcgaccag
      541 gaccgtcccg ccgttctcac cgatcagttt cgcggtgccg tcggcatcgt cgctggccac
      601 gtagacggtc cacaccgtgg gctggctctc gctctggcac gggccgatgg cagccgccgc
      661 gcgaccgccg acctggcaga tctggtaatg gccgaactcc tctccgctgt cgaagaaaga
      721 ccagccgaag accgcgccgt agaactcctt ggcggcggcg aggtcgggta cggacagctc
      781 gacccagcac ggcgtgccgg gggacatggg tcagttcggg taggcatgat gaacctctac
      841 gtcggggatc ggacgcccgc cagcaaagca ccgaccaccg acaaaccaga agttcgccga
      901 ctccaactcg gtaccgacca ccgccgcgcc cgccgcatcc ggcaggccga tccaccgtga
      961 ctccacgtgg ccggcgctgg cgtcggcggc ctgggcacgg attcgaccat tttcgcggtg
     1021 caggagaact ctagtggtaa aacaccaagg tgccgttgag cgacaccagc ggaaaggtgg
     1081 ctttatctgc aaataaatca gtcataatac atcattaccg tccaggcata ctactgcgat
     1141 tcgtgcaata cgcgaataaa gtagatgtgc ctttcccggt ggcgatgcca cgtacatacg
     1201 gcgtgggcca tcgggacgcc gaactggcga tcacccccgc ctgcttggcc gagtggctgc
     1261 gtcgccactg ccgttgccgc gatagacggt gccaccggcg agggcgcaac catcacggtt
     1321 gcgccctcga tgtgcttgtg agccacggca tcagcggtag ctgggtctct accgcaccgc
     1381 agtcagccgg atattagctg gttagcggtt cacacgacgt cggccgtagg caccggcggc
     1441 cagcgcaatt ccgacggcgg cgatcgccac ctgcatcacg atctcgatcc agtcgatgcc
     1501 gcgtgtgaca gcgaccccga ggtactgggc gatcaaggtg cccagtaagg cgccgatggc
     1561 gccgatgaca atggtgagcc agatggggat ggcctgcttg cccggcagga ccaggcgggc
     1621 tagcacaccg atgatcaagc cgacaatgat gccggtgaag atgccggtga ctgtcatgct
     1681 gctcctccgt ccggtaaagg catgagggct gtaccacatg tctcattcat cgcatacccc
     1741 ctcaagcccg acatcacacc ttcgcgcccg taggtgccgg aagggtgggt cgtattgcga
     1801 tccccgagac ggctacggcg actcctctac ctgatggcca gggtgcgcgc cgtgtggcgt
     1861 atgcgcttgc cgtcgaggcg cttgctcaat g
//
LOCUS       DATADN010000665         4095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1276185, whole genome shotgun sequence.
ACCESSION   DATADN010000665 DATADN010000000
VERSION     DATADN010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276185"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VHH53_08610"
     CDS             <1..191
                     /locus_tag="VHH53_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEX2290239.1"
                     /translation="IRELFDMSATRYYQILNGLIDRQDALVADPMLIKRLRRLRAGRQ
                     RARAASRLGVEPRQQSQT"
     gene            complement(188..388)
                     /locus_tag="VHH53_08615"
     CDS             complement(188..388)
                     /locus_tag="VHH53_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290240.1"
                     /translation="MTEGAADALPGVWVEVAGGGPVSEARSPQVTASTPIRTAITANA
                     SSASPAVRVRLLDPVPLGVFIR"
     gene            447..809
                     /locus_tag="VHH53_08620"
     CDS             447..809
                     /locus_tag="VHH53_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX2290241.1"
                     /translation="METPEIPTAGPPARGPGSMASDKGHSRGEVRVYNNSTIRGLAAR
                     AADDLTAAGWTVVEVGNYAQGTIPTTTVYYQEDTDQRDGAEALGAEFDMRVEPRFPGI
                     ANADPGLIVIVTNDYVSP"
     gene            complement(820..3013)
                     /locus_tag="VHH53_08625"
                     /pseudo
     CDS             complement(820..3013)
                     /locus_tag="VHH53_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            3154..3756
                     /locus_tag="VHH53_08630"
     CDS             3154..3756
                     /locus_tag="VHH53_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX2290242.1"
                     /translation="MSTIDRTTRPGSLRVSDAEREQVAGRIRDAATVGMLTLAEANQC
                     QAAAYAAQIRADTVMTAKAQLSGRPRKFVRATHVAVAAGWLGLVVAMLTLGTSAATAS
                     YPMFALACYTMMERIGGAVIPFFAVATLLTGIVLSVATPWQLLQYWWIVVKLVLARAV
                     IVTFLWAATTISVDKPWGKIKRRQHSAVHQREPAGVIGQY"
     gene            complement(3709..>4095)
                     /locus_tag="VHH53_08635"
     CDS             complement(3709..>4095)
                     /locus_tag="VHH53_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX2290243.1"
                     /translation="DPDELLHGWLDVAGLRREVLVPAGPAGCGQVLPRLWDSRVDRAY
                     RAQRVELAANGVLLVAGELLLGRGLPGELTVHLQLSAAALARRLDPQLHWTLPAYRRY
                     DAEHDPAGADVLVLADDPRRLALMHR"
BASE COUNT          645 a         1442 c         1425 g          583 t
ORIGIN      
        1 ccatccgcga gctgttcgac atgtccgcca ctcgctacta ccaaatcctc aacggcttga
       61 ttgatcggca ggacgcgctc gtcgccgatc cgatgctgat caagcgactg cgtcggctgc
      121 gggcgggtcg ccagcgagcc agagccgcta gccggctcgg cgtcgaaccg cgccagcaga
      181 gccagaccta gcggatgaac acccctaatg ggactggatc cagcaggcgg acgcggaccg
      241 ccgggctggc gctgctggca ttagcggtga ttgccgtcct gatcggggtg ctggctgtca
      301 cctgtggaga ccgagcctcg gacaccgggc caccgccggc cacctcgacc catactccag
      361 gcagcgcgtc ggcggcgcct tctgtcattc catttccacc gccagtgacg tcaagtcccg
      421 ctgaagcact cgaaacgccc actcccgtgg aaacccccga aatccccact gccggcccgc
      481 cggcccgcgg gcctggctca atggccagcg acaagggtca cagtcgcggc gaggtgcggg
      541 tctacaacaa cagcaccatc aggggcttgg ccgcgcgcgc cgcggatgac ctcactgccg
      601 cgggttggac ggtggtcgag gtcggcaact acgcgcaggg aacgatcccc acgaccaccg
      661 tttactacca ggaggatact gatcaacgcg acggcgctga ggccctcggt gccgagttcg
      721 acatgcgcgt cgaaccccga ttcccgggca tcgcgaacgc cgatcccggc ttgatcgtga
      781 tcgtcacgaa cgattatgtg tccccgtgat cgctaagcat cagctgggtc gtccggcagc
      841 gtagctggcc agagcggcga gctgaaccgg atccagggac gggcggacgg cttggcgtgc
      901 agcggccagc tgcgccgcgg tcacctcggt ggcttccagc gattcgcgca tggctgtgag
      961 cgcggcctca cggatcagcg ctgcgcagtc cgctgcggag taacccgcca ggtccgcggc
     1021 cagcgcgccg aggtcgacgt cggccgccag cggggtgttg cgggccgcgg atctcaggat
     1081 cgccgtgcgt gcgtcagcgt ccggtggcgg cacgtacacc agacgctcca ggcggcccgg
     1141 ccgtagcagt gccgggtcga tgagttcagg gcggttggtg gcgcccagca ccaccacgtc
     1201 gcgcaacggc tccactccat cgagctcggt gagcagcgtg gccaccactc ggtcggtaac
     1261 gccagtgtca gaagacctac cgcgccgggg ggccagcgcg tcggcctcgt ccaggaaaat
     1321 cagcgcgggt gcggcgtcgc gagcgcggtg gaacagttcg cgcaccgcac gctccgactc
     1381 gccgacccac ttgtccagca attcggcgcc cttgacggag aacacgttga gggctccgga
     1441 tccggccaac gcgcggacta gaaaggtctt gccacagccg ggtggtccgt agagcagcac
     1501 gccgcgcggc gggtcgatgc cgagccgggt gaacgagtcc gggtagcgca gcggccacag
     1561 caccgcctcg gtgagcgcct gcttggtctg ggccatgtcg ccgacctcgt ccagcgtcag
     1621 cccaccggtg gtgatggtgt ccgagccaga catcgagatc gggcggacgg tatccagcgc
     1681 gccgaccaga tcctgctggt tgatcaccag gtcaggcgtg ccgccctcgg cgtctgttgc
     1741 agcatgtcgt aacgcggccc gcacggctgc ctcacggcgc agtgcgacga gatcagcggc
     1801 aacgaaaccc ggggttcggt cacctacggc tgccagatcg acgtccgcgg ccagcggtac
     1861 ctcacgcagc agcacccgga gtagctccgc gcgctcgcgg gcatcgggca acccgattga
     1921 cagctcacga tcgatcaggt ccggtgcgca gccgcggatc aatcgattcg gggtgtgcgg
     1981 aggtgacgac gagcgcgacg ctgtccagcg cgggcgccag catgtcgagt acgacggtgg
     2041 ccagcggcgg cggggcggtg gcgggcagca gtgcctcgac atcggtgacc agcagcacgc
     2101 tgggctgccg cgcgcgggca gccgtcaccg cgtcaccgac ccgctgggcg gccgcgccgg
     2161 gttccaccac tgccacgctc ggggcggcca actcgacaac gtgggctgcg gcgtctcggg
     2221 ccaccgcgcg caccatggtg gccttgccga ccccctcagg cccgtggacc gctaccccga
     2281 ggcgggtgct ggcgcccagc ctgcgcagca ggtcgggctg gcggaaggtg aggtcgaacc
     2341 attcggccag ccgccgggcg gcgtcttgcg cgcccaccag atcggctacg gcgggcgcgg
     2401 ccggtggcgg cgaagcgggg tcccgggatt gctctggcag gggaaggtcc ccggtgcggg
     2461 ggccgtcgcg ccagccgacc acagtggatg gatgcggggc caccggtccg accgggtcgg
     2521 tggccgtcac ggtgagcagc tcgttggtcc aggtggtccc gatcgccgcg gacagccggc
     2581 cgcgcaccgc cgtcacgtcc gagccgggcg ccacgtcctg gggaagcagg gacaccgcgt
     2641 caccggtggc gagcaccttg ccggccagcg ccaaccgcag cgtctcggga ctcaccgaat
     2701 ggctgaccag cgccgaaccg ctgacggtga tcgatcgagc cgcgggcacc tgcgccggtg
     2761 tgagcaccac ttcggagccc tcggtcagcc cgagattgga cagcgtgaga tcgtccagga
     2821 gggcaattcc aggagcggtc tgcccggctg ccgccagtgc tgcgcttacc cgagcgccgg
     2881 tgagccgcac ggcatcccac ggtcgcaagc ccagggcgtc gagcacttcg gggtgtagcc
     2941 ggactacccc gcgccgcgcg tccagcgccg aggtgctgag cctcgcggtc aaggtgatct
     3001 gcggtgtgct cacggcgtcc gagcctacga ggctcacgcc acatcccggg tctgttgcgc
     3061 agcctggaat taccgcagtc tgcatccaga cggcaaaagg gccgggcgcg ggagtaggta
     3121 cgcgtagccg caagatccac gatgagcaac gccgtgtcta ccatcgaccg aacgacccgc
     3181 ccgggctcgc tacgcgtctc cgacgccgaa cgggaacagg ttgccggccg catccgtgat
     3241 gccgccacgg tcgggatgct caccctcgcc gaggcaaacc agtgccaagc cgccgcctac
     3301 gcggcccaga tccgtgccga cactgtgatg accgctaagg cgcagctttc cggccggccg
     3361 cgcaagttcg tccgcgcgac gcacgtcgcc gtggcagcgg gatggctggg cctcgtcgtt
     3421 gcgatgctta ccctggggac cagcgccgcg accgcctcgt atccaatgtt cgcgctcgcc
     3481 tgctacacga tgatggaacg catcggcggc gcggtgatcc ccttcttcgc ggtggcgacg
     3541 ttgctgactg ggattgtcct gtcggtcgcg accccctggc agctgctcca gtattggtgg
     3601 atcgtcgtca agctggtcct cgctagggcc gtcatcgtga cgttcctgtg ggctgccacc
     3661 acgatctccg tggacaagcc ctggggcaag atcaagcggc ggcagcactc agcggtgcat
     3721 cagcgcgagc cggcgggggt catcggccag tactagcacg tccgcaccgg ccgggtcgtg
     3781 ctcggcgtcg tagcggcggt aggccggcag cgtccagtgc agctgcgggt ccagcctgcg
     3841 ggccagcgcg gcggcggaca gctgcaggtg cacggtgagc tcgcccggca gaccccgccc
     3901 gagcagcagc tccccggcca ccagcaagac cccgttggcc gccagctcga cgcgctgcgc
     3961 ccgatacgca cgatccaccc ggctgtccca cagccggggc agcacctggc cgcaaccggc
     4021 gggaccggcc ggcacgagca cctcgcggcg cagccctgct acgtccagcc acccgtgcag
     4081 cagctcgtcg ggatc
//
LOCUS       DATADN010000666         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_66836, whole genome shotgun sequence.
ACCESSION   DATADN010000666 DATADN010000000
VERSION     DATADN010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66836"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VHH53_08640"
     CDS             complement(<1..889)
                     /locus_tag="VHH53_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2290244.1"
                     /translation="MSTVYRGTDMRLERPVAIKVMDPRFAADPTFLQRFEREARAAAR
                     LHHPGVVAVHDQGVDFAGDHAFLVMELVEGATLRDLMRQHGRLPVPLAITVAEMVLSA
                     LAAAHREGLVHRDIKPENVLIGPGGVVKVADFGLVRAAAEASSTGDVILGTVAYLSPE
                     QVATGTADARSDVYATGVLLFEMLTAAPPYGGETALSVAYRHVNDDMPAPSTAGADVP
                     AALDDLIVQATRRDPAVRPSDASAFLRDLQAVRTGLGIDRVAVPLPTSTWSMAAAGPP
                     TAVAGLPTDHGPSGTRALSR"
     gene            1081..1446
                     /locus_tag="VHH53_08645"
     CDS             1081..1446
                     /locus_tag="VHH53_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HEX2290245.1"
                     /translation="MSDIPLAPDVLDPEVVVLPLPDVAQRLGLPVTRVHQMLRDGQLL
                     GVRRHGVAVVPAEFLSGNAVVKGLPGTITLLRDAGYSVEEILRWLFTAEDSLPGTPIG
                     ALRTDRGREVKRRAQAMGF"
     gene            1577..1783
                     /locus_tag="VHH53_08650"
     CDS             1577..1783
                     /locus_tag="VHH53_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEX2290246.1"
                     /translation="MFRRLGKLAALAGIAQAACRYAKNNPEKVHKLADQAGRFVDERT
                     KGKYHNQIDGAVRKVQDAANRRPQ"
     gene            1915..2481
                     /locus_tag="VHH53_08655"
     CDS             1915..2481
                     /locus_tag="VHH53_08655"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2290247.1"
                     /translation="MAVNSYMVPLGTPAPPFALPSLDDQTVASDDLGPGRPLLVMFLS
                     NHCPYVRHIERELGSLVAEYSQRDVAAVAICSNDTKTHPDDGPDGLSGQVHRAGFTFP
                     YLLDESQEVALAYRAACTPDFFLYDAGRKLAYRGEFDESRPRNDAPVTGKALRAALDL
                     VLAGQPVTEPQQPSMGCGIKWKPGNEPR"
     gene            complement(2596..2910)
                     /locus_tag="VHH53_08660"
     CDS             complement(2596..2910)
                     /locus_tag="VHH53_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290248.1"
                     /translation="MGSRQLGSLMFETLLIVVLLIVTVPPTAQQSREPGTGLADDLVF
                     VLVSGLMFGRNGIIFYRRLGRRPATTSRRAAEPGAGQSDHGAVGVPTIPRVLIRFHGQ
                     AD"
     gene            3067..3171
                     /locus_tag="VHH53_08665"
                     /pseudo
     CDS             3067..3171
                     /locus_tag="VHH53_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416939.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
BASE COUNT          528 a         1055 c         1078 g          510 t
ORIGIN      
        1 ccctggacaa ggcccgagtc ccacttggcc catgatcggt gggcaggccc gccaccgcgg
       61 tgggtggtcc cgcggcggcc atcgaccacg tcgacgtggg taggggaacc gcaacccggt
      121 cgattcccaa gccggtccgg accgcctgca ggtcgcgcag aaacgccgac gcgtccgacg
      181 gacggactgc tggatcccga cgggtggcct gtacaatcag gtcgtccaac gctgcgggca
      241 cgtcagcgcc tgccgtgctc ggggcaggca tgtcatcgtt gacgtggcga taggccaccg
      301 acagggcggt ttccccgccg tatggcggcg cggcggtaag catctcgaac aacagcacac
      361 cggtggcgta gacgtcgctt cgggcatcgg ccgtcccggt cgcgacctgc tctggcgaga
      421 ggtaggccac cgtgccaagg atgacgtcgc cggtgctgct ggcctcggcc gccgcgcgaa
      481 ccagtccaaa gtcggccacc ttcaccacgc ccccgggacc gatgaggacg ttctccggtt
      541 tgatgtcgcg atgaaccagt ccttcccggt gcgccgcggc cagcgccgag agcaccatct
      601 cggccaccgt gatggccagc ggcaccggca gcctgccgtg ctggcgcatc aagtcccgca
      661 gcgttgcgcc ctccaccagt tccatcacca agaacgcgtg gtccccagcg aagtcgacgc
      721 cctggtcatg aactgcaacc acgccgggat ggtgcagccg cgcagccgcg cgggcctccc
      781 gttcgaagcg ctgcaggaag gtcgggtcgg cggcgaaccg ggggtccatc accttgatgg
      841 cgaccggacg ctccagcctc atgtccgtgc cccggtagac ggtggacatg ccgccgcggg
      901 ccagtacggg accgacccgg tagcgcccgt caagcagggt gccgaccagg ttcccgtcgt
      961 gtgcgggccc ctgtcgttcc acagttccgg aatggtaagg gcaaggtctg gatcgtatgg
     1021 acccggcgct gcccgaacgg cggaagggcg ccagctggcg ggtatggcat ggtgaccact
     1081 gtgagcgaca tccctctcgc cccggacgtg ctcgatcccg aggtggtggt gctgccgcta
     1141 cccgatgtgg cgcaacggct tggactgccg gtaacccgag tgcaccagat gctgcgcgac
     1201 gggcaactcc tcggggtgcg ccgccacggc gtggcggtgg tgcctgcgga gttcctgagc
     1261 ggtaacgcgg tggtcaaggg actgcccggc acgattaccc tgctacgcga cgccggctac
     1321 tccgttgaag agatcctgcg ttggttgttt accgcggagg actcgttgcc tggcaccccc
     1381 attggcgcgc tgcggactga ccgtggccgc gaggtgaagc gccgcgccca ggcaatgggg
     1441 ttctgattgg gtactgattg gaggcctctg attggggacc tcaatggggg catctgatgg
     1501 ggcgcggtat cctctcgatc aatttctcag tcgtctagct ggggactgtt tgctgggtgg
     1561 caaagggagg gcactgatgt tcagaaggct cggaaagctt gcggctcttg cggggatcgc
     1621 gcaggccgct tgccggtatg ccaagaacaa ccccgagaag gttcacaagc tcgccgacca
     1681 ggctggccgt ttcgtcgacg agcgcaccaa gggcaagtac cacaaccaga tcgatggtgc
     1741 ggtgcgtaag gtccaagacg ctgccaaccg gcgtcctcag tagcgggttc gaagccggcc
     1801 ccgtcgaatc ggtccacggt gctgaaactg ttcggcactg ttcgacgggg ccgcgaagct
     1861 cgcagatcgc ccttcccagc ccttggcttg agcagcgatg agagactggc gaccatggca
     1921 gtaaactcat acatggtccc gctcggcacg ccggcgccgc cgtttgccct gccgtccctg
     1981 gacgaccaga ccgtcgcctc cgatgatctt ggtcctggtc gcccactgct ggtgatgttt
     2041 ctgtccaatc actgccccta tgtccggcac atcgagcggg agctcgggtc gctggtcgcc
     2101 gagtacagcc agcgagacgt ggccgcagtg gcgatctgca gcaatgacac caaaacccat
     2161 cccgacgacg ggcctgacgg gctgagcggg caggtccacc gcgctgggtt caccttcccg
     2221 tacctgctcg acgagagcca ggaagtcgcc ctggcctacc gggccgcctg caccccagac
     2281 ttcttcctct atgacgctgg gcgcaagctc gcctaccggg gcgagttcga cgagtcccgc
     2341 ccgcgcaatg acgcgcccgt gaccggcaag gcgctgcgcg ccgcgctgga tctggtgcta
     2401 gccgggcagc cggtcactga gccgcagcag cccagcatgg gttgcggcat caaatggaag
     2461 ccgggtaacg agccgcgcta acgggcctgg tggtcgatag cgcatcgtcg aagggtgcca
     2521 gtgacatcgt cccgtatcac cgcctaactg taaccacggc gatacccccc gttacagggt
     2581 attgacacgg gccgcctaat ctgcctggcc gtggaagcgg atcagaactc gggggatggt
     2641 cgggacgccg acggccccgt ggtcggactg accggcgccc ggttcggcgg cgcggcggga
     2701 cgtcgtcgcc gggcggcggc cgagccgccg gtagaagatg atgccgttgc ggccaaacat
     2761 caagcccgac accagtacga agaccaggtc gtcggccaga ccggtgcccg gttcccggct
     2821 ctgttgagcc gttggcggca cagtcacgat gagcaggacc acgatgagca gggtctcgaa
     2881 catcaggctt ccgagctgcc gacttcccat cagcaggatg ctggacagca ggaccctgta
     2941 gagcaggttg ggaagaccgc cgtcgcgcca ccaccgccgg tcgcgccgcc accgccggtc
     3001 gcgccgccgg cgcccgagcc tgccggggca aaggatattg acgaggccgc ctgggacagg
     3061 tcggcggaga cttcggactt ggtgcgtcct tacttctgga ccgggggacg gaccgcggcg
     3121 caggtcgacc ttgcgatcga ggccctggtc tcggcaaccg gccgcccact c
//
LOCUS       DATADN010000667         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_686137, whole genome shotgun sequence.
ACCESSION   DATADN010000667 DATADN010000000
VERSION     DATADN010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686137"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1695)
                     /locus_tag="VHH53_08670"
     CDS             complement(238..1695)
                     /locus_tag="VHH53_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX2290249.1"
                     /translation="MTEVSTPVPRIGPSDTHAPPAAPAKSRYDRSIIEGPLGPAVWKM
                     AWPTMVTNIVGGLQGIVDHVLVGHLVGYRANAAIGVSWQIMLVVIIFISSLFTGMSVL
                     VARFAGAGDSDKVDRVVYQAFLTAIFIALAIMAPVGYFASPWLLDLVNATPEVKAEAL
                     PFLRIMFLFSSGMLVFYMLSGALRSAGDARTPMVFGVALTVLNLVLNLILIRGLGPIP
                     AFGTAGSAMGTVIASGLLSGYALWRLWRGGWVVSFPRHHGFAPDWGIIKSLFKFGLPA
                     GIQGIAMNVGGVVMLGFIGSLAQSAEAQAAFAVSYTQLFSLITWTSVGLMGAAAAVAG
                     QNLGAANPDRAERAVHIAARYGLGGAAFLGFFFLFFPRQLLAIFGMDEPVAVEIGVEL
                     LRVLSLSGFFIAAALTYTGGLQGTGDTKSPLYISIVSQILVPLGICFAVQQLGTLDPI
                     DIWIAILVGHMTRCALSVVRFKQGRWRSIAVDINK"
BASE COUNT          352 a          596 c          580 g          257 t
ORIGIN      
        1 aacaggccga gcaggatgag tggtccgccc actgtctcca gcagtccggc gatgccgggc
       61 agcgatgtaa gcggtgccgt tcctccatcg ggcatcactg gagcggggaa cccgaggatc
      121 ttggcagtcc ccacctgggt gaacatgaac cctaccacga tccgaaggac gctcagcaga
      181 tacggcgacc acgagcgcca cctcgttgca atcccaggcc gggtcatgct gcccccctta
      241 cttgttgatg tccactgcga tgctgcgcca ccgcccctgt ttgaaacgga ctacgctgag
      301 cgcgcaccgg gtcatgtggc cgaccaggat ggcaatccag atgtcgatcg ggtccagcgt
      361 ccccagctgc tgcactgcga agcagatccc cagtggaacc aggatctggg agacgatcga
      421 gatgtagagc gggctcttgg tgtcgccggt gccctggagg ccgccggtgt aggtgagcgc
      481 ggccgcgatg aagaagcccg ataggctgag cacccgcagc agctccactc cgatctccac
      541 cgcgaccggc tcgtccatcc cgaagatcgc gagcagctgg cgggggaaga acaggaagaa
      601 gaagccgagg aaggccgcgc cgcccagtcc gtagcgcgcc gcgatgtgca ccgcccgctc
      661 cgcccgatcc gggttcgcgg cgccgaggtt ctgcccggcc accgcggccg ctgcgcccat
      721 cagtcccacc gaggtccagg taatgagaga gaacagctgg gtataggaga ccgcaaacgc
      781 cgcctgggcc tccgcgctct gcgccagcga tccgatgaag cccagcatga caacgccgcc
      841 aacgttcatg gcgatgccct ggattccggc cggcagcccg aatttgaaca gcgacttgat
      901 gattccccag tccggcgcaa atccgtggtg gcgggggaaa gacaccaccc agccgccgcg
      961 ccagaggcgc cagagcgcgt agccggagag aagtccggac gcgatcaccg ttcccatcgc
     1021 cgagcccgcg gtgccgaacg ccggaatcgg acccagcccg cggatcagga tcaggttgag
     1081 caccaggttg agcaccgtga gcgccacgcc gaagaccatg ggcgttcgcg catcgccggc
     1141 ggagcggagc gccccgctca gcatgtagaa caccagcatg ccgctgctga agaggaacat
     1201 gatgcggaga aagggaagcg cctccgcctt cacctcgggc gtcgcgttca ccagatcgag
     1261 cagccagggc gacgcgaagt agccgaccgg cgccatgatg gcgagagcga tgaagatcgc
     1321 ggtgaggaag gcctggtaga ccacccggtc caccttgtcg gaatcgccgg ccccggcaaa
     1381 tcgcgccacc agcacgctca tcccggtgaa cagggaggag atgaagatga tgaccaccag
     1441 catgatctgc cagctcacgc cgattgcggc gttggcccgg tagcccacca gatggccaac
     1501 cagcacgtgg tcgacgatgc cctgcagccc gccaacgatg ttggtgacca tcgtgggcca
     1561 ggccattttc cagactgccg ggcccagcgg accctcgatg atcgaccggt cgtaccggct
     1621 cttggccggc gcggccggtg gagcatgggt atcggacggc ccaatccgcg ggacaggcgt
     1681 ggagacttcg gtcatgcgct cctccagaac gggtctcccg ccggctcttc aactatgacg
     1741 gcaccggggc agagttgctg ttacctactc gtcgaactgg gtgcc
//
LOCUS       DATADN010000668         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_539328, whole genome shotgun sequence.
ACCESSION   DATADN010000668 DATADN010000000
VERSION     DATADN010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539328"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VHH53_08675"
     CDS             <1..817
                     /locus_tag="VHH53_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2290250.1"
                     /translation="RYKRNRLALVGLGIVLVLAFIAIFEPFITPYDPKEQNLLNVRQR
                     PNADHWFGTDILGRDLFSGILYGTRLAMIVGLSVVLLSLIVGLALGAISGYRGRVTDT
                     LVMRTTDIFLAFPFLIGAILVVKIFGDSVQAVIIALAVLSWPTSARLMRGQVLALRES
                     EYVEAARSIGASDTRIVLRHILPNAVAPVLIYAFTSIGVAVVAMASLAFLGIGVPADE
                     PEWGRLIQQAIQVHQIPGMDYLWIFPAVAISITTLGFAFVADGLRDSLDPKLR"
     gene            831..>1508
                     /locus_tag="VHH53_08680"
     CDS             831..>1508
                     /locus_tag="VHH53_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2290251.1"
                     /translation="MAGTADIDLESPAAPLLEIRDLRVHFKTDYGIVKAVDGVSWSVN
                     EGETMAIVGESGSGKSVSAMTILGLVQSPPATFPSGDILLRGRSLMKMPEKQQRKLRG
                     KDVAMIFQDPLTALNPVFKVGDQIAEMVRTHQKVGKLVAKRRALDLLGEVGIPNPAVR
                     LNQYPHEFSGGMRQRAMIAMALANDPQVLLADEPTTALDVTVQAQIMALLTKLQESRN
                     TAIVLITH"
BASE COUNT          235 a          514 c          501 g          258 t
ORIGIN      
        1 gcgctacaag cgcaaccggc tggccctcgt cggtctgggc atcgtgctgg tcctggcgtt
       61 catcgcgatc ttcgagccgt tcatcacgcc gtacgatccc aaagagcaga acctcttgaa
      121 cgttcggcag cggcccaacg ccgaccactg gttcggcacc gacatcctcg ggcgggacct
      181 gttctccggc atcctgtacg gcaccaggct ggcgatgatc gtcggcctca gcgtggtcct
      241 gctgtcgctg atcgtcgggc tcgccctcgg cgccatctcg ggctaccgcg gtcgggtcac
      301 cgacacgctt gtcatgcgta ccaccgacat cttcctggcg ttcccgttcc tcatcggcgc
      361 catcctggtg gtgaagatct tcggggacag tgtccaggcc gtgatcatcg ccttggccgt
      421 gctctcctgg ccgacctcgg cgcggttgat gcgcggccag gtgctcgcgc tgcgcgagtc
      481 ggagtacgtc gaggccgccc ggtcgatcgg cgcgtcggac acccgcatcg tgttgcggca
      541 catcctgccc aacgcggtcg cgccggtact gatctacgcg ttcaccagca tcggggtggc
      601 cgtggtcgcc atggcgtcgc tggcctttct gggcatcggc gttccggccg acgagccgga
      661 gtggggccgg ctgatccagc aggccatcca ggtgcaccag atccccggca tggactacct
      721 gtggatcttc ccggcggtgg ccatctccat cacgaccctg ggatttgcct tcgtggccga
      781 cggcctgcgc gactccctcg accccaagct gcggtaagga gagcgcgccc atggcgggaa
      841 cggctgacat cgacctggag agtccggcgg cgccgctgct ggagatccgc gaccttcggg
      901 tgcacttcaa gaccgactac ggcatcgtca aggccgtcga cggggtcagc tggagtgtca
      961 acgagggcga gaccatggcc atcgtgggcg agtccgggtc cggcaagtcc gtgtcggcca
     1021 tgacgatcct gggcctcgtg cagagcccgc cggcgacctt cccgtccggc gacatcctgc
     1081 tgcgcggccg gtcgctgatg aagatgccgg agaagcagca acgcaagctg cgcggcaagg
     1141 acgtcgcgat gatcttccag gacccgctca ccgcgctgaa cccggtgttc aaggtgggtg
     1201 accagatcgc cgagatggtg cgcacgcacc agaaggtcgg caagctggtg gccaagcggc
     1261 gagcgctgga cctgctcggt gaggtcggca tccccaaccc cgcggtgcgg ctcaaccagt
     1321 acccgcacga gttctccggc ggtatgcggc aacgcgcgat gatcgccatg gcgctggcca
     1381 acgacccgca ggtgctgctg gccgacgagc cgaccaccgc gctggacgtg accgtgcagg
     1441 cgcagatcat ggcgctgctg accaagctgc aggagagtcg caacaccgcg atcgtgctga
     1501 tcacgcac
//
LOCUS       DATADN010000669         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_393253, whole genome shotgun sequence.
ACCESSION   DATADN010000669 DATADN010000000
VERSION     DATADN010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393253"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..750
                     /locus_tag="VHH53_08685"
     CDS             370..750
                     /locus_tag="VHH53_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290252.1"
                     /translation="MTEGKRQLRLQVDDEDADPEELAQLTARLRDELLELDVEAVERP
                     REGSPPPGTRAVELVAVGTLLASFAKPELLTGIVAAIRSWLGRSGQRTVKLELDGDLL
                     ELTGVSSEEQRRLTDEWLRRHTAR"
     gene            758..>1623
                     /locus_tag="VHH53_08690"
     CDS             758..>1623
                     /locus_tag="VHH53_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX2290253.1"
                     /translation="MDGKRSALILASYDYEDPGLQLLRAPARDAEALARVLRDPEIGG
                     FEVRTVLNQPAHMANLAVEEFFAERRPDDLLVLHFSGHGVKDDSGELYFATADTKLRR
                     LGSTTVSAGFVNRQMSRSRSRRVVLLLDCCYAGAFERGMAPKADQAVHVEDQFGGRGR
                     AVITASGAMEYAFEDAELTATSELRPSVFTRAVVEGLESGDADRDQDGFVGLDELYDY
                     IFDRVHEVTPNQTPGKWTFGIQGELHIARRRGSATKPVALPEGLRQALDQPLVGIRLG
                     AVQELERLLGAH"
BASE COUNT          263 a          526 c          575 g          259 t
ORIGIN      
        1 gacttcacgt cgtcccagac cagcgcgtgc ctaggcctga agtcgcgata gctgccagcg
       61 ccgccatctg cgttgccgtc ctgggtgcgc tgctgtcctt atcaacagct cccagctcca
      121 acgccgacag gtgcggctgg agcggctcaa tgcgcaactc cgggacttca acggccccct
      181 gtacgcgatc agggggtgat gaccagcccg gcggcgcagc agccgaaggt gatgagccag
      241 aacgaccaag gaacgtgctg ccgtgccaga cagcacaaac gctttccagc tcattgcgct
      301 tcctttacca ttcgacagat atcgtcagga acgcctgccg cctggctttg tggggggagg
      361 agggcggcga tgaccgaagg gaaacgtcag cttcggctcc aggtcgacga cgaggacgct
      421 gatccggaag agctcgccca actgacggct cggcttcgcg atgagctgct cgagctcgac
      481 gtggaagcgg tcgaacgacc acgggaggga tcgccgccgc cgggaacgcg agcggttgag
      541 ctcgtggcgg ttggcacgct gctcgcgtcc tttgccaagc cggagctgct cacagggatc
      601 gtcgctgcga ttcgctcctg gcttggccga agcggtcagc gcaccgtcaa gctcgagctg
      661 gacggtgacc tgctggagct gaccggggtg tcgtcggagg agcagcggcg tcttaccgac
      721 gagtggctgc gccgccacac ggcccgatga gccggcgatg gacggcaagc gctccgccct
      781 gatcctcgcc agctacgact acgaggatcc agggctccag ctgttgcggg ccccggctcg
      841 cgatgccgag gcgctggcgc gggtgctgcg ggatccggag atcggtggct tcgaggtgcg
      901 cacggtgctg aaccagcccg cccacatggc caatctggcg gtggaggagt tcttcgccga
      961 gcgtcgcccc gatgacctgc tggtgctcca cttctccggc cacggcgtga aggacgacag
     1021 cggggagctg tatttcgcca ccgccgacac aaaactccgc cggctggggt cgaccacggt
     1081 gtcagccggc ttcgtcaacc ggcagatgag ccgcagccgc tcccggcggg tcgtgttgct
     1141 gctggactgc tgctacgccg gcgcgttcga gcggggcatg gcgcccaagg ccgaccaggc
     1201 ggtccacgtc gaggaccagt tcggtggacg gggtcgagcg gtcatcaccg cttcgggggc
     1261 gatggagtac gcgttcgagg acgccgagct gaccgccacc agcgagcttc ggccgtcggt
     1321 gttcaccagg gcggtcgtgg agggtctgga gtcgggggac gccgaccgtg accaggatgg
     1381 cttcgttggc ctggacgagc tctacgacta catcttcgac cgggtgcatg aggtcacgcc
     1441 gaatcagacg cccggcaagt ggaccttcgg catccagggt gagctgcaca tcgcccgccg
     1501 ccgcggatcg gccaccaagc cggtggcgct cccggagggg ttgcggcagg cgctggacca
     1561 gcccctggtc gggatccgcc tcggggccgt gcaggagctg gagcggctcc ttggagccca
     1621 cca
//
LOCUS       DATADN010000670         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_224337, whole genome shotgun sequence.
ACCESSION   DATADN010000670 DATADN010000000
VERSION     DATADN010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224337"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1214
                     /locus_tag="VHH53_08695"
     CDS             45..1214
                     /locus_tag="VHH53_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2290254.1"
                     /translation="MRHSLDAGLFQQTAHSPSYFELSRRMGEHGRQLVDFCIPCNPYF
                     PTPGMFDELAANLELMLKFYPSSADTITNQLCRVLGLNPATVAMANGSTELITWMDHL
                     WVTESLAIPIPTFGRWTDQPMETGKRVDMFPLREVDFFQLNVDEYVRFIRQRGSRVAV
                     VCNPNNPDGGYLPRREVLRFVDALDDLDLVVVDESFVDFVDAERYPSVGPDAAIRPNV
                     VVLKSLGKNFGLHGIRFGYLVANPGLAGTMRRTLPKWNLNSLAETVVYMLAQHGGEYR
                     ESLRLLARDRYNMAMTLAQLPELTVYSSQANFLLVKLAAGINGTELRDYLIGRHQVFI
                     RECGNKLGMSNQFLRLVVRPQQDVSRLVTGITGFAQPNWDDELSRRRNAPASRSA"
     gene            1368..>1567
                     /locus_tag="VHH53_08700"
     CDS             1368..>1567
                     /locus_tag="VHH53_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290255.1"
                     /translation="MRLRSSPSRADGKAEQFLLAAAELIDTYLQVGPIDPDRSARLRD
                     IRFPSALDWLRAEDVIRLAGSRV"
BASE COUNT          259 a          522 c          514 g          272 t
ORIGIN      
        1 cttgggacgg agtaggggat ggggcgagcg acaggagtga tcgggtgcga cacagcttgg
       61 acgccggtct gtttcagcag acggcgcaca gcccgtccta cttcgagctg tcgaggcgga
      121 tgggcgagca cggccggcag ctggtcgact tctgcatccc ctgcaacccg tacttcccca
      181 cgccggggat gttcgacgag ctggcggcca acctggagct gatgctcaag ttctacccga
      241 gcagcgcgga cacgatcacg aaccagctgt gccgggtgct tggcctcaac ccggccacgg
      301 tcgccatggc caacgggtcg accgagctga tcacgtggat ggaccacctg tgggtgaccg
      361 agagcctggc catcccgatc ccgacgttcg ggaggtggac cgatcagccg atggagaccg
      421 gcaagcgggt cgacatgttc ccgttgcggg aggtcgactt cttccagctg aacgtcgacg
      481 agtacgtgcg cttcatccgc cagcgcgggt cgcgcgtcgc cgtggtgtgc aacccgaaca
      541 accccgacgg cggctacctg ccgcgccggg aggtgctgcg cttcgtggac gcgctggacg
      601 acctcgacct ggtcgtcgtc gacgagtcgt tcgtcgactt cgtggacgcc gagcgctacc
      661 cctcggtggg ccccgacgcc gcgatccggc caaacgtcgt ggtgctcaag agcctgggca
      721 agaacttcgg cctgcacggc atccggttcg gctacctggt ggccaacccg ggcctggcgg
      781 gcacgatgcg ccgaaccctg cccaagtgga acctcaactc cctggccgag accgtcgtgt
      841 acatgctcgc ccagcacggc ggcgagtacc gggagagcct gcggctgctg gcccgcgacc
      901 ggtacaacat ggcgatgacg ctcgcccagc tgccggagct gaccgtgtac tcctcccagg
      961 ccaacttcct gctggtgaag ctggccgcgg gcatcaacgg caccgagttg cgcgactacc
     1021 tcatcggccg gcaccaggtc ttcatccgcg aatgcggcaa caagctgggc atgagcaacc
     1081 agttcctgcg cctggtggtc cggccgcagc aggatgtctc ccggctggtc accgggatca
     1141 ccgggttcgc ccagccgaac tgggacgacg agctgtcccg gcgccgcaac gccccggcgt
     1201 ccaggagcgc ctgatggaag gtgcggtcac cggggcagca gcgggaaagg cgtcacccgg
     1261 cggtggccgg tacttagctt aggctgattg ggtcctttcc tcattcccca ttcattccta
     1321 ggctgactaa tgccgcccat gtcacactgc tctccctgga ggaagcggtg cgccttcgat
     1381 cgtcgccgtc ccgtgctgac ggcaaagccg aacaattcct cctggccgcc gctgaattaa
     1441 ttgacaccta tctgcaggtc ggacccatcg atcctgatcg gtcagcccgg ttgagggata
     1501 tcaggtttcc ttcagcgctg gactggttgc gcgctgaaga tgtcatccgc ctggccgggt
     1561 cccgggt
//
LOCUS       DATADN010000671         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_539943, whole genome shotgun sequence.
ACCESSION   DATADN010000671 DATADN010000000
VERSION     DATADN010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539943"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..436)
                     /locus_tag="VHH53_08705"
     CDS             complement(152..436)
                     /locus_tag="VHH53_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290256.1"
                     /translation="MIVDCDRCQVRGNACQDCVITVLLGAPPGAVELDGAERQALDTL
                     AEAGMVPYLRLVDLAEEHNTMPVRTATGVRFGSGAERSMTAGERSTRYVG"
     gene            complement(551..1645)
                     /locus_tag="VHH53_08710"
     CDS             complement(551..1645)
                     /locus_tag="VHH53_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX2290257.1"
                     /translation="MTGPAPDVDVREQETPRVVSHRLSRAARGVAAGVVVAAVFGMSA
                     LPAAADPAPGPAPDPARTVEEVRELSHQAEVITEQWHLATDRLLARRADADRARSDAA
                     AATATADQARAAQQAFRAQVDRLTSATFEGVRISSLTAMLISDSPQQFMEQMSALDVL
                     AVSNRTAMDKLSDAVARTEQAETAATDAETRAAVAELDAGRIAGDVAQARSEMGRRID
                     AVQARLGRLTPDELARYSSEGFTDFPLDIAGDGLGVRALRAALTRQGAPYVWGAEGPG
                     TFDCSGLMLWAYRQAGVSLPRGSWDQARIGTPVVSGLMPGDLIALYSPVSHIGMYVGN
                     GLYLHAPQSGDVVKVARVPWHVVTAIRRVG"
BASE COUNT          313 a          631 c          554 g          278 t
ORIGIN      
        1 cctccaggct tggaagcagc taccgctaag tcgcggcaag gttacgaaag gtcacttgcc
       61 ctgtccacca ccgacgcacc cgcaatgggg cgaagtgact tccggacacg gttggcgtca
      121 ccgcaaccgc accagtgttc agccggcccg atcagcctac gtagcgtgtc gaccgctcac
      181 ctgcagtcat cgaccgctca gcgccactgc cgaaccggac accggtagca gtgcggaccg
      241 gcatcgtgtt gtgctcttcg gcaagatcaa ccaggcggag atagggcacc atgccggcct
      301 cggcgagggt gtccagcgcc tgccgttcgg caccatccag ctcaacggcc cccggcggcg
      361 ctccgagcag aacggtgatg acgcagtcct ggcaagcgtt gccacgcacc tggcaccggt
      421 cgcaatccac gatcatgtcg ggctcccgtc tcactcgtga tctcgtcacg acggacgcta
      481 gatcgtcaca ctgacaatct cgggaacgcg aggctggtta cccggtagat cccgaccaag
      541 ggagaggtga tcagccgacc cgccggatcg cagtgacgac gtgccacgga accctggcga
      601 ctttgaccac atcgccactc tgcggcgcgt ggaggtacaa cccgttcccc acgtacatac
      661 cgatgtggct cactggcgag tacagcgcga tcaggtcacc tggcatcagc cccgaaacca
      721 ccggggtgcc aatcctggcc tggtcccagc tacccctggg caggctcacc ccggcctggc
      781 ggtaggccca cagcatcagg cccgagcagt cgaaggtccc gggaccttca gcaccccaga
      841 cgtatggtgc gccctgcctg gtcagtgccg cccgcagcgc cctgaccccg agcccgtcgc
      901 ccgcaatgtc gagcgggaag tcagtgaatc cctccgagga gtaccgagcg agttcatccg
      961 gagtgagccg gccaaggcga gcctgcaccg catcgatgcg acggcccatc tcgctccggg
     1021 cctgggccac atcgccggct atccggccgg cgtccagttc cgcgacggct gcccgggtct
     1081 cggcgtcggt ggcagcggtc tctgcctgct cggtacgggc cacggcgtcg gaaagcttgt
     1141 ccatcgccgt tctgttgctc accgccagca catccagcgc agacatctgc tccatgaatt
     1201 gctggggcga gtcgctgatc agcatcgcgg tcaggctgct gatccggacg ccctcaaaag
     1261 tcgcgctggt cagccggtca acctgggcgc ggaacgcctg ctgcgcagcc cgggcctggt
     1321 ctgcggtggc cgtcgccgct gctgcgtcac tacgagcccg gtcggcatct gcccggcggg
     1381 ccagcaaccg gtcggtggca agatgccact gctcggtgat cacctcggcc tggtgactca
     1441 gctcccggac ctcttcaacg gtcctggccg gatcgggcgc agggccgggt gccgggtcgg
     1501 cggccgcggg gagggcggac atgccgaata cagcagcgac cacaacacct gccgccacgc
     1561 cgcgagcagc gcggctcaga cgatgggaca ccacccgcgg cgtctcctgt tctcggacgt
     1621 caacgtccgg tgccggaccg gtcacgggag atcatcccgc gaccggagta acggccctca
     1681 ggaaccatgg acgcggatct tcactcgaac agtcgactta cagcacccga acgggtgctt
     1741 aaagcatgta ccaatgcgct ccgtactcgt tagcgc
//
LOCUS       DATADN010000672         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_393449, whole genome shotgun sequence.
ACCESSION   DATADN010000672 DATADN010000000
VERSION     DATADN010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393449"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VHH53_08715"
     CDS             <1..1217
                     /locus_tag="VHH53_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2290258.1"
                     /translation="DPRYYMDYTGTGNSLNVRQPHTLQLIMDSLRYWVTEMHVDGFRF
                     DLASTLAREFYDVDRLSTFFDLVQQDPVISRVKLIAEPWDVGPGGYQVGNFPPLWTEW
                     NGKFRDTVRDFWRGEPGTLGEFGSRLTGSSDLYQADGRRPHASINFVTAHDGFTLTDL
                     VSYNDKHNEANGENGQDGADDNRSWNCGVEGLTDDPEVVDLRARQRRNMLATLFLSQG
                     IPMLLHGDELGRTQQGNNNVYCQDNELSWVDWSAVDQELFTFTAGLIALRRDHPVFRR
                     RRFFAGRPIRGGDELGDIAWFTESGEKMTEQDWEVEVCQCLTVFLNGEGIPDADGRGQ
                     RVTDNSFLLCFNAHHENTIVQLPGNGYGQQWTVVLDTATGEPPGTRGATAAGEDKITV
                     TARSLVVLERTA"
     gene            1214..>2176
                     /locus_tag="VHH53_08720"
     CDS             1214..>2176
                     /locus_tag="VHH53_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2290259.1"
                     /translation="MTLPSSTYRLQLGPDLSFADAAAVVPYLASLGAGAVYTSPLLQA
                     MPGSQHGYDVADPRRVSDVIGGEPGRRVLVAAVREHGLALVVDIVPNHLGVADPQVNP
                     AWWDLLQHGPASRFARWFDITGWPLRLPVLADSSDAFSDLRVEGELLRYHERAFPLAP
                     GTAAAGSPLDVHEAQHYRLVSWRADLGYRRFFAVSELAAVRVEDPEVFAATHGEVLRW
                     VAGGDVDGLRVDHPDGLTDPAGYLHRLREHAPDAWILVEKILEPGEELPGSWPVAGTT
                     GYDALAEVDGVLVDPAGEMAFTALDTSLTGVRTDWAQLVHDCKLD"
BASE COUNT          344 a          750 c          728 g          354 t
ORIGIN      
        1 acgacccgcg ctattacatg gattacaccg gcaccggaaa ctcgctcaat gtacgtcaac
       61 cgcacaccct tcagctgatc atggattcac tgcggtactg ggtcaccgag atgcacgtcg
      121 acggcttccg cttcgacctc gcctccacgc tggcccgcga gttctacgac gtggaccgcc
      181 tgtccacgtt cttcgacctg gttcagcagg atccggtgat cagccgggtg aagctgatcg
      241 ccgaaccgtg ggacgtcggg ccagggggtt accaggtggg caacttcccc ccactgtgga
      301 ccgaatggaa cggcaagttc cgtgacacgg tgcgcgactt ctggcgcggc gagccgggca
      361 cgctaggcga gttcggctcc cggctgaccg gttcctccga cctctaccag gccgatggcc
      421 gccggccaca cgcgtcgatc aacttcgtca ccgcgcacga tgggttcact ctcaccgacc
      481 tggtctccta caacgacaaa cacaacgagg ccaacggcga aaacggtcag gacggcgccg
      541 acgacaaccg gtcctggaac tgcggggtgg agggcctcac cgacgacccc gaagtagtgg
      601 atctgcgagc ccgccaacgg cgcaacatgc tggccacctt gttcctgtcc caaggcatcc
      661 ccatgctgtt gcacggtgac gagctcggcc gcactcagca gggcaacaac aacgtctatt
      721 gccaggacaa cgagctttcc tgggtggact ggagcgcagt cgatcaggaa ctgttcacgt
      781 tcaccgccgg gctgatcgcg ctacgccgcg accacccggt gttccgccgc cgcaggttct
      841 tcgccgggcg cccgatccgg ggcggtgacg agctgggcga catcgcgtgg ttcaccgaat
      901 ccggcgagaa gatgaccgag caggactggg aggtggaggt ctgccagtgc ctcaccgtgt
      961 tcctcaacgg cgagggcatc ccggacgccg acggccgtgg ccagcgggtc accgacaact
     1021 ccttcctgct ctgcttcaac gcccaccacg agaacaccat cgtgcagctg cccggcaacg
     1081 gctacgggca gcagtggacc gtcgtgctgg acacggcgac cggcgaaccg ccgggcaccc
     1141 gcggtgccac cgccgcggga gaggacaaga tcaccgtcac cgcgcggtcg ctggtagttc
     1201 tggagcgcac cgcgtgacgc tgccatcctc gacctaccgg ctgcagctgg ggccggatct
     1261 gtccttcgcc gatgccgccg cggtggtgcc ctacctggcg tcgctgggag ccggcgcggt
     1321 gtacacctcg ccgctgctgc aggcgatgcc gggttcccag cacggttacg acgtggccga
     1381 cccccgccgg gtgtctgacg tcatcggcgg agagcctggc cggcgcgtgc tggtggctgc
     1441 ggttcgcgag cacgggttgg cgctggtggt tgacatcgtg cccaaccacc tcggtgtcgc
     1501 cgatccgcag gtcaacccgg cctggtggga cctgctgcag cacgggccgg cgtcgcgctt
     1561 cgcccggtgg ttcgacatca ccggctggcc gctgcgcctg cccgtcctgg ctgactcctc
     1621 ggatgcgttt tccgacctgc gggtggaggg cgaactgttg cgctatcacg agcgcgcgtt
     1681 cccgctggca cccgggacgg cagcagccgg tagcccactg gatgtgcacg aggcacagca
     1741 ctatcggctc gtctcctggc gggcggacct gggctaccgg cggttcttcg cggtcagtga
     1801 gctcgccgcg gtccgagtgg aggatcccga ggtcttcgcc gccacccacg gcgaggtgct
     1861 gcgctgggtc gccggcggcg acgtcgacgg cttgcgggtc gatcacccgg atggcctcac
     1921 cgacccagcg ggctacctgc accggctgcg ggagcacgcc ccggacgcct ggatcctggt
     1981 ggagaagatc ctcgaacctg gtgaggagct gcccggctcg tggccggtgg cgggcaccac
     2041 cggctatgac gcgctcgccg aggtcgacgg ggtcctcgtc gacccagccg gcgaaatggc
     2101 gttcaccgcg ctggacacct cactgactgg ggtccggacg gactgggcgc agctggtgca
     2161 tgattgcaag ctcgac
//
LOCUS       DATADN010000673         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_393559, whole genome shotgun sequence.
ACCESSION   DATADN010000673 DATADN010000000
VERSION     DATADN010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393559"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1610)
                     /locus_tag="VHH53_08725"
     CDS             complement(69..1610)
                     /locus_tag="VHH53_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2290260.1"
                     /translation="MTEKHGRYSRREVLQLSGRAVGLAAAGALVAGCTSAVTQPPPAP
                     VIDPVNLDPLKLTIDMIRFDTSHNGEGGVTVPHAEMLKSIWDAAGAHTEIIPTPKPDN
                     AHFTARVRSAVGSARPLLLLCHSDVVSVEADRWTVDPYRGEVGDGWIYGRGALDMKGT
                     NAAFMAALLRHLSEGAQFDRDIIFLADCDEEAGPHSTRWLAERLYDKIDAGAVITEGG
                     WALAPPGSVVPVVVTLTRQDKMSAALELVARGTTTHSSRPSPDAAITRLNRAAVRLAD
                     YQPGVGLTDVTRPYFEAWASRTDNPKLAAALRLMLNASEQAERDRAGALVVQLSNHPT
                     LHNALMRHIVTPVIQRAGYRVNIQPGTATASLNLRLVPGGLDVAATVAQMRDALGASD
                     GVTLRLVNPLSPDQTEEQVLDGLAQRMAQPPGSIDTDVFRALEAAARETYPDAAVTSG
                     LFEAGTSASPWRQRGIPVYGIYPYATDDDTMSRMHGNDERIRVDALARGTELMYRVFN
                     RFRAT"
     gene            complement(1666..>2907)
                     /locus_tag="VHH53_08730"
     CDS             complement(1666..>2907)
                     /locus_tag="VHH53_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX2290261.1"
                     /translation="DCVHCGFCLPSCPTYVLWGEEMDSPRGRIHLMQTGLDGEPLSES
                     MVGHFDACLGCMACVTACPSGVQYDTLITDTRAQVERRYERSRPDRLLREAIFRLFPY
                     PRRLRALRGPLALYQRSGLDRLLRRSGLLDRLPPTLRVMESLTPKLTRREKLPQQVAA
                     KGRRRAVVGMLTGCVQDAFFPEVNAATARVLAAEGCDVVIPHEQGCCGALSLHTGRED
                     EAVHFARALIERFEVAGVDYVVVNAAGCGSAMKEYAHLLRDDPDYAARAQAFVDRTRD
                     VSELLVELGPVAPRHRLDVTIAYHDACHLGHAQGIRSQPRELLRAIPGLTMTEIAEAD
                     LCCGSAGIYNLLNPEPATELGDRKARNVLATGASVLVTANPGCLMQIAASAKRLDQHI
                     ALAHTINVLDASIRGAPLRSS"
BASE COUNT          450 a          973 c          994 g          490 t
ORIGIN      
        1 tgcatagtca cgtttttcgg acaatgcgcg tggcctcctg tgaacaacgc ccccgatctg
       61 atttcgatct aggtcgcgcg gaaccggttg aacactcgat acatcagttc ggttccgcgc
      121 gccagcgcgt ctacccggat ccgctcgtcg ttgccgtgca tccgcgacat cgtgtcgtca
      181 tcggtggcat aggggtagat cccgtacact gggatgccgc gctgccgcca cggacttgcg
      241 ctggtacccg cttcgaacag gccggaggtg acggccgcgt cggggtaggt ctcacgagct
      301 gcggcttcca gtgctctgaa gacgtcggta tcgatgctcc ccggcggctg ggccatccgt
      361 tgtgcgagcc cgtcgagcac ctgttcctcg gtctggtcgg gcgacagcgg gttcaccagg
      421 cgcagcgtca cgccgtcact ggcacccagc gcgtcgcgca tctgcgccac cgtcgcagcc
      481 acatcaagac cgcccgggac cagtcgcagg ttcaggctgg cggtcgccgt cccgggctgg
      541 atgttgacgc ggtaaccggc gcgctggatg accggagtga cgatgtggcg catcaaggcg
      601 ttgtgcagcg tcgggtggtt gctgagctgg acgaccagcg cgccggcgcg gtcgcgctcg
      661 gcctgctctg aggcgttgag catgaggcgc agcgcagcgg ccagcttcgg attgtcagtc
      721 cgcgaagccc atgcttcaaa gtacggccgg gtcacgtcgg tgaggccgac tccaggttgg
      781 taatcggcca gccggaccgc cgctcggttc agccgggtga tggccgcgtc cggcgacggg
      841 cgggaagagt gggtggtggt tccccgggcg acgagctcca gcgcggcaga catcttgtcc
      901 tgccgggtca gcgtgaccac caccggcacc accgagccgg gcggagccag cgcccatccc
      961 ccctcggtga tcaccgcccc ggcgtcgatc ttgtcgtaaa gccgctcggc gagccaccgg
     1021 gtgctatgcg ggccggcttc ctcgtcgcag tcggcgagga agatgatgtc ccggtcgaac
     1081 tgggcgccct cactgaggtg ccgcagcagg gccgccatga acgcggcgtt ggtgcccttc
     1141 atgtccagtg caccgcggcc gtagatccag ccgtcaccta cctcgcctcg gtagggatcg
     1201 acggtccagc ggtcggcctc cacggacacc acgtcggagt ggcacagcag cagcagcggc
     1261 cgtgcgcttc ccacggcgct gcggacgcgg gcggtgaaat gggcattgtc cggcttcggg
     1321 gtcggaatga tctcggtgtg tgccccggcc gcgtcccaga tcgacttcaa catctcggca
     1381 tgcggaacgg tcacgccgcc ctcgccgttg tgtgacgtgt caaaccggat catgtcgatg
     1441 gtgagcttca gcgggtccag gttcaccggg tcgatgaccg gcgctggggg cggttgagtg
     1501 accgcgctgg tgcagccggc caccagggcc ccggctgcag ccagccccac tgctcgaccg
     1561 ctgagctgca gcacctcgcg ccggctgtac cggccgtgct tttcggtcac gagtccctcc
     1621 ccgacggctc gtgagtgcgt acagtgtcat aacactgtta cgcattcacg agctgcgcag
     1681 cggtgcccct cggatcgagg cgtccagcac gttaatggtg tgcgccagtg cgatgtgctg
     1741 gtcaagacgt ttcgcgctgg ccgcgatctg catcaggcat ccaggattgg cggtgactaa
     1801 caccgacgcc ccggtggcga gcacgttgcg ggccttgcga tcccccagtt cagtggccgg
     1861 ctcggggttg agcaggttgt agattcctgc cgagccgcag cacaggtcgg cttcagcgat
     1921 ctcggtcatg gtcagcccag ggatagcccg cagcagctcc cgcggctggg atcggatgcc
     1981 ttgggcatgc ccgaggtggc aggcgtcgtg gtaggcgatc gtgacgtcca gccggtggcg
     2041 cggggcgacc gggccgagct cgacaagcag ctcggagaca tccctggtgc ggtcgacgaa
     2101 agcttgcgca cgggccgcat agtcgggatc gtcgcgcagc aggtgtgcgt attccttcat
     2161 cgccgagccg cagcccgctg cattgaccac gacgtagtcc accccggcga cctcgaagcg
     2221 ctcgatcagc gcccgcgcga agtggaccgc ctcgtcctcg cgacctgtgt gcagcgacag
     2281 cgccccgcag cagccctgct cgtggggaat cacaacgtcg cagccctcgg cggccagcac
     2341 tcgggcggtg gcggcgttga cttcaggaaa gaacgcatcc tgcacgcagc cggtgagcat
     2401 gccgaccact gcgcggcggc gacctttggc cgccacctgc tgtggcagtt tctctctccg
     2461 ggtcagcttc ggagtcaatg actccatcac ccgcagcgtc gggggcagcc ggtccagtaa
     2521 tccgctgcgg cgtagcagcc ggtccagccc gctgcgctga tagagagcca gcggtccgcg
     2581 cagcgcccgc agccgtcgtg ggtacgggaa cagccggaag atcgcttcgc ggagcagccg
     2641 gtccgggcgg gaccgctcgt agcgacgctc cacctgggct cgagtgtcgg tgatcaaagt
     2701 gtcgtattgc acgcccgagg ggcaggccgt cacacacgcc atgcacccca ggcaggcgtc
     2761 gaagtgcccc accatcgact cggacagcgg ctcgccgtcc aggccggtct gcatcaggtg
     2821 aatgcgcccg cgtggagagt ccatctcctc cccccacagc acgtaggtgg gacaggaggg
     2881 caggcagaac ccgcaatgca cgcagtc
//
LOCUS       DATADN010000674         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_393726, whole genome shotgun sequence.
ACCESSION   DATADN010000674 DATADN010000000
VERSION     DATADN010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393726"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1030
                     /locus_tag="VHH53_08735"
     CDS             215..1030
                     /locus_tag="VHH53_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2290262.1"
                     /translation="MSEDQELIIRANIAVQTGDGGDAHEALRLFEQLLPDQQRVLDSD
                     HPDVLTTRHNIAYWTGQAGDARAALELFEQLLPDERRVLGEDHPAVLTTRANIAYWTS
                     QVGNARAALELFEEMLPDQRRVLGADHPAVLTTRANIAYWTGQVGDARAALELFQQLL
                     PDQQQVLGADHPNVLATRHNIAHWTGEVGDARAALELFEKLLPDRQRVLGADHPAVLA
                     TRYNIAARTGEVGDARAALKLFEKLLPDQRRVLASDHPDVLSTQKWIRYLKQD"
     gene            complement(1448..>2091)
                     /locus_tag="VHH53_08740"
     CDS             complement(1448..>2091)
                     /locus_tag="VHH53_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX2290263.1"
                     /translation="IEYTRRDLSAKVDALAEKVNPSRAMQRKVDHARTSMISIKDKIM
                     GTAEDTASSTADAVNSAASDVAERTSSVASDVTERASSAASKATDMVASIPQAARRQA
                     QGNPLAAGLIAFGAGWLVSSLLPPSRKERELASQATNAVSQQVQPLAQQLGQTAQGMA
                     QNLQKPAQQAAESVQATASRAASTITDQSRSAAEDIAGRAEQAKNSVGDSARS"
BASE COUNT          358 a          650 c          673 g          410 t
ORIGIN      
        1 gctgggcatc gacccgtcct tcgcccagcc catccagacg ccactgccgc ctgtggggtg
       61 cccgtgctgg gacgccgcag tgcccctcgt cggacatcca tttctgccca gttggggccc
      121 cgccaaacgc cagcccaccg ctgtgcttcc gacaccgctg gtccttcatt tgcttcgggt
      181 agatccttag cggttcatcc ggtagggatg gagcatgagt gaggatcaag agctgatcat
      241 ccgcgccaac attgcggttc aaaccgggga cggaggggat gctcacgagg cgctgcggtt
      301 gttcgagcag ttgttacccg accagcagcg ggtgctggat tccgatcatc ccgacgtgct
      361 gaccacccgc cacaacatcg catactggac tgggcaagcc ggggatgctc gcgcggcgct
      421 ggagttgttc gagcagttgt tgcccgacga gcggcgggtg ctgggtgaag atcaccccgc
      481 cgtgctgacc acccgagcca acatcgccta ctggactagc caggtcggca acgctcgcgc
      541 ggcgctggag ctctttgagg agatgttgcc tgaccagcgg cgggtgctgg gtgcagatca
      601 ccccgccgtg ctgaccaccc gagccaacat cgcctactgg actggccagg tcggggacgc
      661 tcgcgcggcg ctggagctgt tccagcagct gttgcctgac cagcagcaag tgctgggtgc
      721 agatcacccc aacgtgctgg ccacccgcca caacatcgcg cactggaccg gagaggtagg
      781 ggacgctcgg gcggcgctgg agctgttcga aaagttgttg cccgaccggc aacgggtgct
      841 aggtgccgac caccccgccg tgctggctac ccgctacaac atcgcggccc ggactggaga
      901 ggtgggggac gctcgcgcgg cgctgaagct gttcgagaag ttgttgcccg accagcggcg
      961 ggtactggct tccgatcacc ccgacgtgct gtccacacag aagtggattc gttaccttaa
     1021 gcaagactag ctgtggctgc acgaagtgtg ctgaccactg atgccgcatc cgctccgatc
     1081 ctgtgcgacg gaagctgctt taaccaggca ctatggcttg gccaggtttt actcggtgag
     1141 gccaagggga cggcttggcc ggtggctgac cagcagaggc tgtgccatgg ccggagagct
     1201 attcaacctt gatagtgctc aatctcttcg ccgaacgagt cacgaccgaa gtgacgcaaa
     1261 attctcctac tcaatgtccg cgaggtacgc gaagtgcaat ctcgctttta tgtgcggagt
     1321 ctaagagcag gttgtcagtt ccgtgtcaac ggcgctcaca acctacgccc gtagctgatc
     1381 tcaacgtaag agggagggcc gcactccatg tcgattgagc agccataccc tactgacctg
     1441 cgggccttca actgcgagcg gagtcaccaa cggagttttt ggcctgctca gcgcgcccgg
     1501 caatatcttc ggccgcggag cgactctggt cggtgatggt tgatgctgcg cggctggctg
     1561 ttgcttgaac ggactcggca gcctgctgcg caggtttttg cagattctgc gccatccctt
     1621 gggccgtctg accaagctgc tgcgcgagcg gctgcacctg ctggctcacc gcgttcgtgg
     1681 cctggctagc cagctcccgc tccttgcggc tcggaggcag cagcgaggac accagccacc
     1741 ctgccccgaa cgcgatgagg cccgccgcca gcggattacc ttgggcctgc cgccgggcgg
     1801 cttgcggtat tgaggcgacc atgtcagtcg cctttgaggc ggctgaagag gcacgctcgg
     1861 tgacatcgga ggcaaccgaa gaggtgcgct cggcgacatc ggaggcggcc gaattcaccg
     1921 cgtcagcggt cgaggaagcg gtgtcctccg cagtgcccat gattttgtcc ttgatgctga
     1981 tcattgaggt gcgtgcgtgg tcgacctttc gttgcatggc acggctggga ttgaccttct
     2041 cggccagtgc gtccaccttg gcgcttagat cccgtcgggt gtattcgatc t
//
LOCUS       DATADN010000675         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_68308, whole genome shotgun sequence.
ACCESSION   DATADN010000675 DATADN010000000
VERSION     DATADN010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68308"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VHH53_08745"
     CDS             <1..372
                     /locus_tag="VHH53_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290264.1"
                     /translation="AWNGSSGTYGVGAPAGNRQPFGGSAGQAFPQQRGEGTVYGAQQS
                     PIDMTMPVSMNAIENSGSLTGHILAQGWDRGVDANRRSNVKVALAMLVVLLFLVGVSL
                     LFLFTAGESFTDMINDVFNKG"
     gene            625..>1583
                     /locus_tag="VHH53_08750"
     CDS             625..>1583
                     /locus_tag="VHH53_08750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2290265.1"
                     /translation="MTTFADPSTFSSVFDDQTIEIQTPSFAELGLPAELVRVLTREGI
                     TVPFEIQAATIPDALAGRDVLGRGQTGSGKTLAFGLPLLARIARGGKARPHQPKALIL
                     VPTRELAMQVADALMPLGRAVGVFLKTAVGGVPYDRQMDALRRGVEVMVATPGRLADL
                     IERGACRLDQVEVTVLDEADQMADMGFLPEVTELLAKTPEGAQRLLFSATLDGDVDTL
                     VKRFMHDPVTHSTAPVEASVSTMDHHLLLIPPHDKFPITASIANRAGKTIVFARTQMG
                     VDRLVEQLAAVGVRAGALHGGKTQRVRTRTLAEFKEGRSNVLVA"
BASE COUNT          239 a          548 c          548 g          248 t
ORIGIN      
        1 gcctggaacg ggtccagtgg cacgtacggg gtcggcgccc cggccggaaa ccggcagccg
       61 ttcggtggat cggccggtca ggcgttcccg cagcagcgcg gcgaaggcac ggtgtacggg
      121 gcacagcagt ccccgatcga catgaccatg ccggtatcga tgaatgcgat cgagaactcc
      181 ggctcgttga ccggccacat tctcgcccag gggtgggacc gcggcgtgga cgccaaccgg
      241 cgcagcaacg tcaaggtcgc tctcgcgatg ctggtggtgc tgctgttcct ggtgggcgtc
      301 agcctcctgt tcctgttcac cgccggcgag tcgttcactg acatgatcaa cgacgtgttc
      361 aacaagggct gaacgcgttt gccctgggcg ggcgtgcagc ctaggctggc gtggttagcg
      421 ccgaggggtg agcctgccca ctaccggtgc ggaatgcggg cagcgccaaa atcgttgacc
      481 cgtacactga tgagagtaat tgacgcggcg tgccccggtg cacgtcacgg gcgttcttgc
      541 gggcagtaca gcggtcggcc atctccggac ggagcgggcc acggcgggcc attcgcgagc
      601 atgcgccccc gtagagaggt tctcttgacc actttcgctg atcccagcac gttttcgtcc
      661 gtcttcgacg accagaccat cgagatccag acccccagct tcgccgagct cggcctgccc
      721 gccgagctcg ttcgtgtgct gacccgcgag ggcatcaccg ttccgttcga gatccaggcc
      781 gccaccatcc cggacgcgct ggccggccgc gacgtgctcg gccgcggcca gaccgggtcc
      841 ggcaagacgc tggccttcgg ccttccgctg ctcgcccgca tcgcgcgcgg cggcaaggcc
      901 cgtccgcacc agccgaaggc gctgatcctg gtccccaccc gtgagctggc catgcaggtg
      961 gctgacgcgc tgatgccgct cggccgcgcg gtcggcgtgt tcctcaagac cgcggtcggc
     1021 ggagtgccgt acgaccggca gatggacgcc ttgcgccgcg gcgtcgaggt gatggtggcc
     1081 accccgggcc ggctggccga cctgatcgag cgcggcgcct gccggctgga ccaggtcgag
     1141 gtgaccgtgc tcgacgaggc ggaccagatg gccgacatgg gcttcctgcc ggaggtcacc
     1201 gaactgctgg ccaagacgcc ggagggcgct cagcggctgc tcttctcggc caccctggac
     1261 ggtgacgtcg acacgctggt caagcggttc atgcacgacc cggtgacgca ctcgaccgca
     1321 ccggtcgagg ccagcgtgtc caccatggac caccacctgc tgctgatccc gccgcacgac
     1381 aagttcccga tcaccgcgtc gatcgccaac cgcgcgggca agacgatcgt cttcgcccgt
     1441 acgcagatgg gtgtcgaccg tctggtcgag cagctggccg cggtcggcgt ccgggcgggc
     1501 gcgctgcacg gcggcaagac ccagcgggtg cgcacccgga cgctggccga gttcaaggaa
     1561 ggccgcagca acgtcctggt cgc
//
LOCUS       DATADN010000676         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_540406, whole genome shotgun sequence.
ACCESSION   DATADN010000676 DATADN010000000
VERSION     DATADN010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540406"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VHH53_08755"
     CDS             complement(<1..203)
                     /locus_tag="VHH53_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290266.1"
                     /translation="MPLGFGPGQVGVDENAGQVIFCRGLAHSPDGDCIDLVLDVQATP
                     AWRITDVRYWGFPHDRWHLDFDR"
     gene            346..1776
                     /locus_tag="VHH53_08760"
     CDS             346..1776
                     /locus_tag="VHH53_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEX2290267.1"
                     /translation="MELSWFWSPDYSVARFLLERGIALIYLLGFVSALHQFPALLGER
                     GLLPVPRHLAYLPFRQSPSLFHLGYTDGRLRLVAGAGAILSVLLLVGLPQAAPLPMTM
                     AAWLACWVLYLSIVNVGQTFYAFGWESLLLEAGFLAAFLGNAETAPPFLVLLLFRWLA
                     FRVEFGAGLIKMRGDPCWRDLTCMQYHHETQPMPNPLSWWFHRLPRPLHRFEVLGNHV
                     TQLVMPFGLFLPQPFAAIAAGAMLLTQGYLVVSGNYAWLNALTIVIAFSAVPDFAFGP
                     LETWVAGPVADGPAWFAGAAVALTAGVAILSWWPVRNLLGRRQLMNYSFNPLHLVNTY
                     GAFGSITRERYEVVIEGTRDASPGEEAEWREYEFVGKPGDPRRRPRQVAPYHLRLDWL
                     MWFAPLSAAYAADWLPRLVEKLLTNDRDTLNLMGPNPFADAPPRWIRARLFHYRYSTW
                     SERRRTGAWWVREPAGTLLPPVGGGG"
     gene            complement(1781..>2010)
                     /locus_tag="VHH53_08765"
     CDS             complement(1781..>2010)
                     /locus_tag="VHH53_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2290268.1"
                     /translation="PADLEAYNANYVGGDINGGLQHWAQFFTRPAVRLDPYSTPDPGI
                     FICSSSTPPGGGVHGLCGLYAAESALRGALR"
BASE COUNT          277 a          726 c          667 g          340 t
ORIGIN      
        1 tcacggtcga agtcgaggtg ccatcggtcg tgggggaagc cccagtagcg gacgtcggtg
       61 atccgccagg ctggcgtagc ctggacatcg aggacgagat cgatacagtc cccgtccgga
      121 ctgtgggcca agcctcggca gaagatcacc tggccggcat tttcgtccac gccgacctga
      181 ccgggcccaa acccgagcgg catcagcacc ggatccagta cctgagcaac gagggtcgga
      241 ccaacacctc ccgaccttgg ggacacgcca ggcgacggca gcatcggctg atgctaacgg
      301 ccgcacgaag ggctcggcac gcggcacgct acctttacca ccgacgtgga actgagctgg
      361 ttctggtccc ccgactactc ggtggcccgc ttcctcctcg agcgcgggat cgcgttgatc
      421 tacctgctcg ggttcgtctc ggcgctgcac cagtttcccg cgctgctcgg cgagcggggt
      481 ctgctgcccg ttccccggca cctggcgtac ctgcccttcc gccagtcccc cagcctcttt
      541 cacctcggct acaccgacgg gcgcctccgc ctcgtcgccg gagccggggc gatcctgtcc
      601 gtcctcctgc tggtgggcct gccgcaggca gctcccctcc ccatgaccat ggctgcgtgg
      661 ttggcgtgct gggtcctgta cctgtcgatc gtgaacgtcg ggcagacgtt ctacgcgttc
      721 ggctgggagt cgctcctgct cgaggccggc ttcctggccg ccttcctggg caacgccgag
      781 accgctccgc cgttcctggt gctcctgctg ttccgatggc tcgcgttccg ggtcgagttc
      841 ggcgcgggcc tgatcaagat gcgcggcgat ccatgctggc gcgacctgac ctgcatgcag
      901 taccaccatg agacccagcc gatgccgaac ccgctgtcgt ggtggttcca ccgcctgccg
      961 cggccgctgc accggttcga ggtgctcggc aaccacgtga cccagctggt catgcccttt
     1021 ggcctgttcc tgccgcagcc cttcgccgcc atcgcggccg gcgccatgct cctcacccag
     1081 ggctacctgg tggtgagcgg caactacgcc tggctcaacg ccctgaccat cgtgatcgcg
     1141 ttctctgccg tgcccgactt cgccttcggc ccgctcgaga cctgggtggc cgggcccgtg
     1201 gccgatgggc cggcgtggtt cgccggcgca gccgttgcgc tgaccgcggg tgtcgcgatc
     1261 ctgagctggt ggccggttcg taacctgctc ggccgccggc agctgatgaa ctactcgttc
     1321 aacccgctgc acctggtgaa cacctacggg gcgttcggca gcatcacgcg cgagcgctac
     1381 gaggtcgtca tcgagggcac gcgcgatgcc agccccggcg aggaggcgga gtggcgggag
     1441 tacgagttcg tcggcaagcc cggcgaccca cgacgccggc cgcgccaggt ggcgccgtac
     1501 cacctcaggc tggactggct gatgtggttc gcaccccttt cggcggcgta cgcggccgac
     1561 tggctgccgc ggctggtcga gaagctgctg acgaacgacc gtgacaccct gaacctgatg
     1621 ggcccgaacc cattcgccga tgcgcccccg cgctggatcc gcgcccgcct cttccactac
     1681 cgctacagca cctggtccga acgccgacgg acgggcgcct ggtgggtccg cgagcccgcc
     1741 ggaacgctcc tgccgccggt cggcggtggt ggttgacggc tcagcgcagg gcgccgcgca
     1801 gcgccgactc cgccgcgtat aggccacaca ggccgtgcac gccaccgccg gggggggttg
     1861 acgaggagca gatgaagatg cccggatcag gcgtcgagta aggatccagc cgcaccgcgg
     1921 gccgtgtgaa gaactgggcc cagtgctgca ggccgccgtt gatgtcgccg cccacgtagt
     1981 tggcgttgta cgcctccagg tccgccggcc
//
LOCUS       DATADN010000677         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1446716, whole genome shotgun sequence.
ACCESSION   DATADN010000677 DATADN010000000
VERSION     DATADN010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446716"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VHH53_08770"
     CDS             complement(<1..1064)
                     /locus_tag="VHH53_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX2290269.1"
                     /translation="MDTGDTAWMLASSALVLLMTPGLAFFYGGMVRSKSVLNMIMMSF
                     SAMGVVGVLWVLYGYSVAFGNDVGGGLLGNPAEFAGLQGTFGGTYLATEGGPPAEIPL
                     VGTIPATVFVAFQAMFAIITVALISGAVADRMKFGAWLLFAGLWATIVYFPVAHWVFS
                     FDGVTAETGGWIANQLKAIDFAGGTAVHINAGIAALVLVLILGKRRGWPHTPMRPHNL
                     PFVMLGAGLLWFGWYGFNAGSAGASGGLAGSTFVTTTVATAAAMLAWLLTERIRDGKA
                     TSLGAASGVVAGLVAITPSCSAVNVLGALVIGVVAGSLCALAVGLKYKLGYDDSLDVV
                     GVHLVGGIVGTLLIGLVAAP"
     gene            1327..>1681
                     /locus_tag="VHH53_08775"
     CDS             1327..>1681
                     /locus_tag="VHH53_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2290270.1"
                     /translation="MTGIPDGAPVAVVAGRYRLRELVGTGGMGAVWRAGDELLGREVA
                     LKRVRLADQATGDVVLARERIMREARIAAALHHPHIVSIFDVVVQDGEPWLVLEFLPS
                     RSLGSVLAERGTLPPV"
BASE COUNT          291 a          566 c          588 g          236 t
ORIGIN      
        1 tcgggggcgg ccaccaggcc gatcagcagc gtgccgacga tgccgccgac caggtgcacg
       61 cctaccacgt cgagcgagtc gtcgtagccc agtttgtatt tcaggccaac cgccaacgcg
      121 cacaacgacc ctgcgaccac accgatcacc agtgcgccga gcacgttgac cgccgagcag
      181 gagggcgtga tcgccaccaa cccggccacc acgcccgagg ctgcacccag cgacgtcgcc
      241 ttgccgtcgc gaatgcgctc ggtaagcaac caggccagca tcgccgcggc agtggcgacc
      301 gtggtggtca cgaaggtgga accggccaga ccgccagaag cgcctgcgga accggcgttg
      361 aagccgtacc aaccgaacca cagcaggccc gcaccgagca tgacaaaggg caggttgtgc
      421 ggtcgcatcg gggtgtgcgg ccagcctcgg cgcttaccca ggatcaacac cagaaccagc
      481 gccgcgatac ccgcgttgat gtgcaccgcg gtgccgcccg cgaagtcgat ggccttgagc
      541 tggttggcga tccagccgcc cgtctcggcg gtgacgccgt cgaaggagaa cacccagtgc
      601 gcgaccggga agtagacgat cgtggcccac agcccggcga acagcagcca tgcgccgaac
      661 ttcatgcggt cggccaccgc accggagatc agcgcgacgg tgatgatcgc gaacatcgcc
      721 tggaacgcca cgaagaccgt ggccgggatg gtgccgacga gcgggatctc ggcaggcggg
      781 ccgccctcgg tggccaggta ggtgccgccg aacgtgccct gcaggcccgc gaactcggcc
      841 gggttcccga gcagaccgcc gccgacgtcg ttgccgaacg ccaccgagta gccgtagagc
      901 acccagagga cgccgacgac gcccatggcc gagaagctca tcatgatcat gttgaggacg
      961 ctcttgctac ggaccattcc gccgtagaag aacgccaggc ccggtgtcat cagcagcacc
     1021 agcgctgagc tggcgagcat ccaagcggta tcgccggtat ccacggatct ccctcccgtc
     1081 tcggccgccc atgccagcgg caagctggga ggaactatct gttcggcgtg tttcggacac
     1141 cctccaccgt tgtttccgcg ccgtgaacga gcgggccgcg gatgttacat ccgggtgacg
     1201 acgtcatctg aggagtctga tggccggggg tcgacagcag ccggggaggc ctcccggcgt
     1261 gggacgcgaa gatcgtccgt ggaatcgttt cgtcctcgat gcggcccaca caggagggag
     1321 tgagggatga ccgggatccc ggatggtgct ccggtcgctg tggtcgctgg ccgttaccgg
     1381 ctgcgcgagc tcgtcgggac cggtggcatg ggcgccgtgt ggcgggcggg cgacgagctg
     1441 ctcggccgtg aggtggcgct gaagcgggtg cgcctggccg accaggcgac gggcgacgtc
     1501 gtgctcgcgc gcgagcggat catgcgcgag gcgcggatcg ccgcggcgct gcaccacccc
     1561 cacatcgtct cgatcttcga cgtcgtggtg caggacggtg agccgtggtt ggtgctggag
     1621 ttcctgccgt cccggagcct ggggagcgtg ctcgccgaac gcggcacgct gccgccggtc
     1681 g
//
LOCUS       DATADN010000678         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1146955, whole genome shotgun sequence.
ACCESSION   DATADN010000678 DATADN010000000
VERSION     DATADN010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146955"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VHH53_08780"
     CDS             <1..1534
                     /locus_tag="VHH53_08780"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX2290271.1"
                     /translation="VLSGTGGGGDPGPTPPIASFTASPTSGTAPLQVSFSDTSSGSPS
                     SWAWDFDNDGTVDATVQHPSYTYAEPGSYTVSLTVANADGSDVETKAGYISVAGAGGG
                     GQALTVIATDDAYVDSASPTSNFGNANNLRVRLSGSKVLHTYLKFSVTGAGSPSSAVV
                     RLHVTDASNHGGDLYLVPNSTWSEASLTWSNRPAAAGSLLHTVGSAAVGTWVEWDVSD
                     VVTADGTYSFVLVNASDDVVRYASSEGTAATRPQLVLSGTGGGGDPGPTPPIASFTAS
                     PTSGTAPLQVSFSDTSSGSSSSWAWDFDNDGTVDATVQHPSYTYAEPGNYTVSLTVAN
                     ADGSDTETKLGYISVAQPGGGGQALTVIASEDTYVDSSSPSSNYGTATNLRVRLSGTK
                     VLHTYLKFSVTGAGSPSSAVVRLHVIDASNHGGDLYLVPNSTWSEASLTWSNRPAAAG
                     SLLHTVGSAAVGSWVEWDVSDVVTADGTYSFVLVNASDDVVRYASSEGTAATRPQLVL
                     SP"
BASE COUNT          266 a          611 c          507 g          209 t
ORIGIN      
        1 cgtgctgtcc ggcaccggtg ggggcggcga tccgggtccg acgccgccca ttgccagctt
       61 cacggccagc ccgaccagcg ggaccgcacc gctgcaggtc agcttcagcg acaccagcag
      121 cgggtcaccc agcagctggg cctgggactt tgacaacgac ggcacggtcg acgcgaccgt
      181 ccagcacccg agttacacct acgccgagcc gggcagttac accgtcagcc tgaccgtcgc
      241 caacgccgac ggctccgacg tcgaaaccaa ggccggctac atcagcgtcg ccggggcggg
      301 cggtggcggc caggcgctga ccgtgatcgc caccgacgac gcctatgtcg actccgccag
      361 ccccacctcg aacttcggca acgccaacaa ccttcgcgtg cgcctgtccg gctccaaggt
      421 gctgcacacc tacctcaagt tcagcgtcac cggtgccggc agtccgagca gcgcggtggt
      481 gcgcctgcac gtcaccgacg ccagcaacca cggcggcgac ctgtacctgg tgcccaactc
      541 gacctggagc gaggccagcc tgacctggtc caaccggccg gccgccgcgg gcagcctgct
      601 ccacacggtc ggtagcgccg ctgtcggcac ctgggtggag tgggacgtca gcgacgtggt
      661 caccgccgac ggcacctaca gcttcgtgct ggtcaacgcc tccgacgacg tggtgcgcta
      721 cgcctcgtcc gagggcaccg ccgccacccg gccacagctc gtgctgtccg gcaccggtgg
      781 gggcggcgat ccgggtccga cgccgcccat tgccagcttc acggccagcc cgaccagcgg
      841 gaccgcaccg ctgcaggtca gcttcagcga caccagcagc gggtcatcca gcagctgggc
      901 ctgggacttc gacaacgacg gcacggtcga cgcgaccgtc cagcacccga gttacaccta
      961 cgccgagccg ggcaattaca ccgtcagcct gaccgtcgcc aacgccgacg gctccgacac
     1021 cgagaccaaa ctgggctaca tcagcgtcgc ccagccgggc ggtggcggcc aggcgctgac
     1081 cgtcatcgcc agcgaggaca cctacgtcga ctcctccagc cccagctcga actacggcac
     1141 cgccaccaac ctgcgcgtgc gcctgtccgg caccaaggtg ctgcacacct acctcaagtt
     1201 cagcgtcacc ggcgccggca gcccgagcag cgcggtggtg cgcctgcacg tcatcgacgc
     1261 cagcaaccac ggtggcgacc tgtacctggt gcccaactcg acgtggagcg aggccagcct
     1321 gacgtggtcc aacaggccgg ccgccgccgg cagcctgctc cacacggtcg gcagcgccgc
     1381 tgtcggcagc tgggtggagt gggacgtcag cgacgtggtc accgccgacg gcacctacag
     1441 cttcgtgctg gtcaacgcat ccgacgacgt ggtgcgctac gcctcgtccg agggcaccgc
     1501 cgccacccgg ccgcagctgg tgctgagccc ctgagcggtc aagactccgg atcgacgatt
     1561 cgagcttcgg tcgtcgcgca cgttgtgagc gcc
//
LOCUS       DATADN010000679         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_540549, whole genome shotgun sequence.
ACCESSION   DATADN010000679 DATADN010000000
VERSION     DATADN010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540549"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VHH53_08785"
     CDS             complement(<1..458)
                     /locus_tag="VHH53_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2290272.1"
                     /translation="MTDGELAVIGLGSAGSMALWQAARLSGGSGVVGFEARTPGHGRS
                     AVGGDSRLFRTTFREQHDLTPLLESSLLLWRELESESGQEILTQCGGLSIGDRNGDYV
                     PAILRNVRETGAPHTVLDAAALRARYPQHRVGDDDAAVFDPRAGFLRTDRA"
     gene            complement(455..1255)
                     /locus_tag="VHH53_08790"
     CDS             complement(455..1255)
                     /locus_tag="VHH53_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2290273.1"
                     /translation="MSELTSESSVERGEGTIVGREIRKSFGSHLVLAGIDITVEAGEI
                     TCIVGPSGSGKSTLLRCINGLETVDSGVLRVNGEDLGFRETPSSYIALPPAALARQRQ
                     RIGMVFQGFNLFPHMTATENVMAGPVLVKGTAKAAATEQATCLLASVGLAGFERHYPA
                     QLSGGQKQRVAICRALAMEPSIMLFDEPTSALDPERVGEVLEVMKDLATKGMTMVVVT
                     HEIGFAREVADTVLFMDEGLVVERGDARAVLSAPKERRTQSFLEKVLT"
     gene            complement(1252..>1524)
                     /locus_tag="VHH53_08795"
     CDS             complement(1252..>1524)
                     /locus_tag="VHH53_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX2290274.1"
                     /translation="VLPPTGNQLISMLKGTSLVSVLGVADLLQSAQLIYARTYETIPL
                     LIVASLWYLAMTILLGIPQSMIERHYGRSDRRPARARRAAQEVPAT"
BASE COUNT          213 a          510 c          558 g          243 t
ORIGIN      
        1 gcacggtccg tgcgcaggaa gcccgcgcgc ggatcgaaca cggccgcgtc gtcgtcgccc
       61 acgcggtgct gggggtaccg ggcgcgcagc gcggccgcgt ccagcacggt gtgcggcgcg
      121 cccgtctccc ggacgttgcg caggatcgcc gggacgtagt cgccgttccg gtcgccgatc
      181 gagaggccac cgcactgggt gaggatctcc tgccccgact cggactccag ctcccgccac
      241 aggagcagcg agctctccag gaggggggtg aggtcgtgct gttcgcggaa cgtcgtgcgg
      301 aacagccggg agtcgccgcc gacggcgctg cgtccgtggc cgggggtccg cgcctcgaac
      361 ccgacgacgc cgctcccgcc cgagagccgc gccgcctgcc acaacgccat gctgcccgcg
      421 ctgcccaggc cgatgaccgc gagctcgccg tcggtcacgt cagcaccttc tccaggaagg
      481 actgggtgcg ccgctccttg ggcgcggaca gcaccgcgcg ggcgtcgccg cgctcgacca
      541 ccagaccctc gtccatgaac agcacggtat cggcgacctc gcgggcgaac ccgatctcgt
      601 gggtgaccac gaccatggtc atgcccttgg tggccaggtc cttcatcacc tcgaggacct
      661 cgccgacgcg ttcggggtcg agcgcgctgg tgggttcgtc gaacagcatg atgctgggtt
      721 ccatggccag cgcgcggcag atcgcgacgc gttgtttctg cccgccggac agctgggcgg
      781 ggtagtggcg ttcgaacccg gcgaggccga cgctggccag caggcaggtg gcctgttcgg
      841 tggctgcggc cttggcggtg cccttgacca ggactggtcc ggccatgacg ttctcggtgg
      901 cggtcatgtg ggggaacagg ttgaacccct ggaagaccat gccgatgcgt tggcgttgcc
      961 gggccagggc cgcgggcggc agtgcgatgt acgacgaggg ggtctcccgg aacccgaggt
     1021 cctcaccgtt gacccgcagc actccggagt ccacggtctc caggccgttg atgcagcgca
     1081 gcaaggtgga cttgcccgac ccgctgggtc cgacgatgca ggtgatctcc ccggcctcga
     1141 cggtgatgtc gatgccggcc agcacgaggt gggagccgaa cgacttgcgg atctcccggc
     1201 cgacgatggt gccctcgcca cgctctacgg atgattcgct cgtgagctcg ctcatgttgc
     1261 tggcacctcc tgtgccgctc ggcgggcgcg ggccgggcgc cggtcggatc gcccgtagtg
     1321 ccgttcgatc atggactggg ggatcccgag caggatggtc atggccaggt accagaggct
     1381 ggccacgatc agcagcggga tggtctcgta ggtgcgggcg tagatcagtt gtgcgctctg
     1441 cagcaggtcg gccaccccga gcacgctgac cagtgaggtg cccttgagca tgctgatcag
     1501 ctggttgccg gtgggtggga ggac
//
LOCUS       DATADN010000680         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_394102, whole genome shotgun sequence.
ACCESSION   DATADN010000680 DATADN010000000
VERSION     DATADN010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394102"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="VHH53_08800"
     CDS             <1..1914
                     /locus_tag="VHH53_08800"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX2290275.1"
                     /translation="YHLGAEGKYYRMFGEGETKVTLTSNPSHLEAVDPVLEGITRAKQ
                     DILNKGPDGFTVLPVALHGDAAFAGQGVVAETLNLSQLRGYRTGGTVHVIVNNQVGFT
                     TAPQYARSSEYSTDVAKMIGAPVFHVNGDDPESCVWVAKLAVDYRQAFQKDVVIDMIC
                     YRRRGHNEGDDPSMTQPKMYDIIDTKRSVRKGYTEALIGRGDISVEEAERVLKDYAQQ
                     LEHVFNEVRELEKSAATPSESVETEQMVPTGLSTGVDRSVIERIADAHVNLPEGFTPH
                     PRVKPVLEKRAKMARDGGIDWAFAELLALGSLCMDGRMVRLSGQDTRRGTFVQRHSVL
                     IDRKTGAEYTPLMNLADDQAKFLAYDSALSEFAAVGFEYGYSVANPDALVLWEAQFGD
                     FVNGAQPVIDEFISSGEAKWGQHSDVVLLLPHGHEGQGPDHTSGRIERFLQLCAEGSM
                     TVAQPSTPANYFHLLRRHALDGVHRPLVVFTPKSMLRLKAAVSPVEDFTSGRFASVLD
                     DPLITDSSAVRRVLLCSGKISYELNATRQQRGISDVAIVRIEQLYPVPKRRLAAALDR
                     YPNATDVRWVQEEPANQGAWPFYGLSLPDLIPGRLGNLRRISRRAMAAPSAGSAKVHE
                     VEQRELITAALER"
BASE COUNT          351 a          677 c          699 g          327 t
ORIGIN      
        1 taccacctcg gcgctgaggg caagtactac cggatgttcg gtgagggcga gacgaaggtg
       61 acactgacgt ccaacccgtc gcacctggag gccgtggacc cggtgctgga gggcatcacg
      121 cgagccaagc aggacatcct gaacaagggt ccggacggct tcaccgtgct gccggtcgcc
      181 ctgcacggcg atgccgcctt cgccgggcaa ggcgtggtgg ccgagacact caacctctcg
      241 caactgcgcg gctaccggac cggcggcacc gtgcacgtga tcgtgaacaa ccaggtgggg
      301 ttcaccacgg cgccgcagta cgcacggtcc agcgagtact ccaccgacgt cgcgaagatg
      361 atcggcgcgc cggtattcca cgtcaacggc gatgatccgg agtcctgtgt gtgggtggcc
      421 aaactcgcgg tggattaccg ccaggccttc cagaaggacg tcgtcatcga catgatctgc
      481 taccgccgcc gcggtcacaa cgagggcgat gacccatcga tgacccagcc gaagatgtac
      541 gacatcatcg acaccaagag gtctgtgcgc aagggctaca ccgaggcgct catcggccgc
      601 ggtgacatct ccgtggagga ggccgagcgc gtactgaagg actatgccca gcagcttgag
      661 cacgtgttca atgaggtgcg ggagctggag aagagcgccg cgacaccgag cgagtcggtg
      721 gagaccgaac agatggtgcc caccgggttg tccaccgggg tggaccgcag cgtcatcgag
      781 cggatcgccg acgcgcacgt caacttgccc gagggcttca ctccgcatcc tcgggtcaag
      841 ccggtgctgg agaagcgggc gaagatggcc cgcgacggtg gcatcgactg ggccttcgcc
      901 gagctgctgg cgctcggctc actgtgcatg gacggccgga tggtgcggct gtccggtcag
      961 gacacccggc gaggcacctt cgtgcaacga cactccgtgc tgatcgaccg caagacgggg
     1021 gccgagtaca ccccactgat gaacctggcc gacgatcagg cgaagttcct ggcctatgac
     1081 tccgcgctgt cggagttcgc tgcggtgggt ttcgaatacg gctactcggt ggcaaacccg
     1141 gacgccctgg tgctctggga ggcgcagttc ggcgacttcg tcaacggtgc gcagccggtg
     1201 atcgacgagt tcatctcctc cggcgaagcc aagtggggac agcattccga cgtcgtgctg
     1261 ctgctgccgc acggccacga agggcagggc ccggaccaca cgtctggccg catcgagcgg
     1321 ttcctgcagc tctgcgctga gggctcgatg accgtggcgc agccatccac gccggcgaac
     1381 tacttccacc tgctgcgccg gcacgcactg gacggcgtgc accggccgct ggtggtcttc
     1441 acgccgaagt cgatgctgcg gctcaaggcg gcggtgagcc cggtggagga cttcacctca
     1501 gggcggttcg cctcggtcct cgacgaccct ctgatcaccg attcatcggc ggtgcgccgg
     1561 gtgctgctgt gcagcggcaa gatcagctat gagctgaacg ccacacgcca gcaacgtggc
     1621 atctccgacg ttgcgatcgt ccgcatcgag cagctctacc cggtgcccaa gcggcggctg
     1681 gcggcggcgc tggaccgcta cccgaatgcc accgacgtgc gttgggtgca ggaggaaccc
     1741 gccaaccagg gcgcgtggcc gttttacggg ttgagcctgc ccgacctcat tcccgggcgg
     1801 ttgggcaacc tgcggcggat ttctcggcgc gccatggcgg ccccctcggc cggatcggcc
     1861 aaggtgcacg aggtggagca acgtgaactc atcaccgccg ccctcgaacg ctgacccgcc
     1921 cccaacccgg cgtgtccggc cctagtgccc ggcgtgtccg gccctgatgt ccggtgtgtc
     1981 cggccctggt gccggcgtgt ccggcgctga tgcatgctgc gccgccgtta tgggcgcagg
     2041 cgcctccgaa gaag
//
LOCUS       DATADN010000681         1748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_225087, whole genome shotgun sequence.
ACCESSION   DATADN010000681 DATADN010000000
VERSION     DATADN010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225087"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1075..>1748
                     /locus_tag="VHH53_08805"
     CDS             1075..>1748
                     /locus_tag="VHH53_08805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2290276.1"
                     /translation="MSGEGELIAGRYRLISRLGSGAMGVVWQAHDERLYRTVAIKQLV
                     LSSTLSDLETKEVTSRAMQEGRITARVQHPHVVTVHDVVEHHGQPCLIMEYLPSRSLA
                     TVLSIHGVLAPNIVAGIGSQIASALAAAHQAGVMHRDIKPGNVLLADDGTAKITDFGV
                     SRAVGDLTVTATGILAGTPAYLAPEVAQGNGGGFPSDVFSLGSTLYTALEGTPPFGLS
                     NNPIALL"
BASE COUNT          305 a          547 c          583 g          313 t
ORIGIN      
        1 cttgggaacc ctatagtgct gccctggtcg aggtcttgac ctacgcacgg ctgtggtcac
       61 gggtgacccg gttggccgtt gctgaccctc tcttgtggca cgcgtgtggc acggcgcgcc
      121 gcacctgtcc ccgccatgaa agacggctcg ccccctccag atggaacgca gcgccaccaa
      181 caccaggtca actgcggtca cctatggcac gtctggccgc aggcagcgcc tgggtcagcg
      241 agacgtgcct tgccggttgg cgaggggagg acagcaccct tcgtggggaa gttgcaccgc
      301 aagagtgaac acgctgacca catgcttaca cagtgaagct ggagccggat tgtccttgtg
      361 ctaatgggac cattgtcact aagcgtggtc gatcgattct tcggattcca catccttgcc
      421 gcggcatcca cagtctcggc gagttgtcca catggtgtgg agggtgttct tgtcggggcg
      481 tccctatggt gtcgcaccgt gagtcagaca ccgatgtatg gccagccgca tggcgagcgc
      541 atcaacgctg atgtttctgc cggtgaggct ggcccgtact tggtcgagta ccccggcaaa
      601 cactgcttct gggataaaac gcccactgtg gtgtgcggcc caccactagg tccaggaatc
      661 gatctcgctg agggctggtc ctggttcaga gcgggaaagc cgggtcgtgc aggtccggcg
      721 aatgccccgc gatcggccca ctgtttagtg gggactcact gtggtgggcg cacaccggcg
      781 gtggatcagc gagaaggctc tgaccagcgg ggagcatcgg agcacacact gcgggctctg
      841 ctttcgccac cagaccgacg tgcaacacgc aacaggtcgg ggcgctcagc gccagcggcg
      901 acaaagccgg tccgctcgaa acgctctcca gagcacacga cctcgcggca gccgcggcca
      961 cggcaggtgc ccggctggtg ctggcggcag cgccgtagag gaggtcggtc tgagatggcc
     1021 gcagcagtag gcgcagcagg caaggtaagt agtccaatgc cgttcggagt tgcagtgagc
     1081 ggcgaggggg agttgatcgc tgggcgttat cgcctgatct cgcggctggg cagtggggcg
     1141 atgggtgtgg tgtggcaggc gcacgacgag cgcctatacc gcaccgttgc gatcaagcag
     1201 ctggtgctgt catcgacgtt aagcgacctc gagactaagg aggtcaccag ccgggcgatg
     1261 caggaggggc gtatcaccgc ccgagtgcag cacccgcacg tggtcacggt ccatgacgtg
     1321 gtggagcacc atggccaacc gtgcctgatc atggagtatc tgccgtccag gagcctggcc
     1381 acggtgctgt ccatccacgg cgtgctggca ccgaacatag tcgccggtat cggtagccag
     1441 atcgcctccg cgctggccgc tgcgcaccag gcgggcgtca tgcaccggga tatcaagccg
     1501 ggcaacgtgc tcctcgcaga tgacggcacc gccaagatca ccgacttcgg tgtctcccgc
     1561 gccgtaggtg acctcaccgt gaccgccact gggatcctcg ctgggacgcc cgcctacctg
     1621 gctccggagg tcgcccaggg caacggtggt ggcttcccct cggatgtgtt ctcgctgggc
     1681 tccaccctct acaccgcgct ggaaggcacg ccgccgtttg gactcagcaa caacccgatt
     1741 gccttgct
//
LOCUS       DATADN010000682         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1278316, whole genome shotgun sequence.
ACCESSION   DATADN010000682 DATADN010000000
VERSION     DATADN010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278316"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VHH53_08810"
     CDS             complement(<1..230)
                     /locus_tag="VHH53_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2290277.1"
                     /translation="MRAIQVTETGDPEVLRLTKLPDPEFGPGQLLVELAAAGVNYIDT
                     YHRSGAYPMPLPFIPGSEGAGTVTAVGPDTGG"
     gene            complement(349..1245)
                     /locus_tag="VHH53_08815"
     CDS             complement(349..1245)
                     /locus_tag="VHH53_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX2290278.1"
                     /translation="MITQGGIAVTGSVVRVTGSGLGCPTWPQCFPGSLVPQPHPEVAA
                     LHQWIEFCNRLLTGLVGLVALACLVAALLIRPRRRRLVLLAATMPLGVALQAVLGGIT
                     VLTGLAWWTVAMHFLVSMVLVWLAVLLVGAFAEGDAPPRWQVPGPVRGLLGTATAVLA
                     ALLVAGTLVTAAGPHAGDATTPRLAIGVPVMAQLHADLLIGFLGLLTGLGFALRAVGV
                     SSRTWWRYLVLMSVVLAQGALGLVQYLTGVPEMLVSLHVLGAASVVVAMAALWTSCRI
                     RDHVADPGARESRRPLAGAGTS"
     gene            complement(1484..2257)
                     /locus_tag="VHH53_08820"
     CDS             complement(1484..2257)
                     /locus_tag="VHH53_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2290279.1"
                     /translation="MTRFSPGTFTPAPGRGQLPRMLLTQARTEFVLTLRNAEQVLLTL
                     LIPLGLLIGLTALAVLPVPDPRVDNVTPRILALAVMSTAFTGQAIALGFDRRYGVLKR
                     LAATALPRWSLVAGRMLAVLGVVLVQLVVLGAVATALGWRPEPAGLVAAGLLVLAGTA
                     AFGALGVLLGGTLRAEVVLAVANTVWLLLVVAGGIALPVQALPGPLSILAAGLPSGAL
                     AEGLHATLVTGVTPWGALGVLLAWAAGAGALAARTLRLS"
     gene            complement(2254..>2610)
                     /locus_tag="VHH53_08825"
     CDS             complement(2254..>2610)
                     /locus_tag="VHH53_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2290280.1"
                     /translation="LMEEAEALADQVVIIDYGRVVAQGSPAELTTAGAQRQLRFGARP
                     GLDLRPLCTALPEGCQASEPHPGGYLVEGLIDPQVMATVTAWCAQQGVLAEDLRVAKR
                     SLEDVFLELTGRELRP"
BASE COUNT          454 a          915 c          925 g          316 t
ORIGIN      
        1 aatcccccgg tgtcgggccc taccgcggtg acggtgccgg caccctccga acccgggatg
       61 aacggcagcg gcattgggta ggcgccgctg cggtgatagg tgtcgatgta gttgacgccg
      121 gcagcggcga gctcgacgag caactggccg ggcccgaact ccgggtctgg cagcttggtc
      181 agccgcagga cttcggggtc gccagtttcg gtgacctgga tcgcgcgcat ggcggcagct
      241 tacgagccgc gggccagggc ccgccgagcc agcccgagcg gcagacaccc ggtacggggg
      301 tatccggtga tcacaccgcc agttgcgatg gcgaggaggg tgaagtcttt aggaggtgcc
      361 tgcgcctgct aacggtcgcc ggctttcccg ggcgcccgga tccgctacat ggtcgcggat
      421 gcggcagctc gtccacagcg cagccatcgc cacgaccacc gaggcggcac cgagtacgtg
      481 caacgagacg agcatttcgg gcaccccggt gaggtattgc acgaggccca atgcgccctg
      541 cgcgagcacc acggacatca gtaccaggta gcgccaccag gtgcggctgg acacccctac
      601 cgcacgcaac gcgaacccca ggccggtgag caatccgagg aagccgatga gcagatcggc
      661 gtgaagttgg gccatcaccg ggacgccgat cgcgagccgg ggagtggtgg cgtcaccggc
      721 gtgcgggccc gctgcggtga ccagcgttcc agcgaccagc agcgccgcca gtaccgcagt
      781 ggcggtgccc agcaagccgc gcacaggacc gggcacctgc cagcgcggtg gtgcgtcgcc
      841 ttcagcgaag gcaccgacca gcagcaccgc cagccacacc agcaccatag acaccaggaa
      901 gtgcatcgcc acggtccacc aggccagccc ggtgaggacc gtgatccccc cgagaacggc
      961 ctgcagcgca acaccgaggg gcatcgtggc ggccagcagt accagccgcc ggcgccgggg
     1021 ccggatcagc agcgcggcca ccaagcaggc tagcgcgacg aggccgacca agccggtgag
     1081 caggcggttg cagaactcga tccactggtg cagcgcggcg acttcgggat gcggctgcgg
     1141 caccaagctg cccgggaagc actgcggcca ggtcgggcag cccagacccg agccggtcac
     1201 ccgcaccact gagccggtca ccgcgatccc cccctgggtg atcaccgcgg cgatcgcgat
     1261 actccgcgac aggcttgcag gtacgcggtg gtgagcgggc gcggtcggca caaccccgat
     1321 gctatgcagc cgccgcaccc gcccgggctt gggtcaccgg tctttgagtg ctctcaactt
     1381 cccaagctca accacagagc tgtctgggtc gagctgaact gctctgtggt tgagctcggg
     1441 agcccatcag tagtcaccgg acttgacggt gcgcagcggg ggctcaggac aggcgcaggg
     1501 tgcgagcggc cagcgcaccg gcaccggcgg cccacgccag caggaccccc agcgcgcccc
     1561 acggggtgac gccggtgacc aaggtcgcgt gcagaccctc ggccagtgcg ccggagggca
     1621 gcccggcggc gagtatggac agcggaccgg gcaaggcttg caccggcagc gcgatgccgc
     1681 ccgcgacgac caggagcagc cacaccgtgt tggcgactgc cagcaccacc tcggcccgaa
     1741 gcgtccctcc gagcagtacc ccgagtgcgc cgaacgcggc ggtaccggcg agcacgagca
     1801 gcccggcggc aaccaggcct gccggctccg ggcgccagcc cagcgcggtg gccaccgccc
     1861 cgagcacgac cagttgtacc aagaccaccc cgagcaccgc gagcatccga ccagcgacca
     1921 acgaccagcg cggaagggcg gtggcggcca gccgtttgag cacgccgtag cggcggtcga
     1981 agcctagcgc gatggcctgg ccggtgaagg cagtcgacat caccgccagc gccaggatcc
     2041 gcggagtgac gttgtccacc cgggggtcgg gcaccggcag caccgccagc gcagtgagcc
     2101 cgatcagcag cccgagcggg atgagcaggg tgagcagcac ctgctcggcg ttgcgcaggg
     2161 tgagcacgaa ctcggtgcga gcctgggtga gtagcatccg cggcagctgg ccccggcctg
     2221 gtgcgggcgt gaaggtgccg ggagaaaagc gggtcatggc cgtagctccc ggccggtgag
     2281 ctcgaggaag acatcctcca ggctgcgttt ggccacccgc aggtcctcgg cgagcactcc
     2341 ctgctgggcg caccacgcgg tcacggtagc catcacttgc gggtcgatca ggccctcgac
     2401 gaggtatccg ccggggtgcg gctcactggc ctggcatcct tcgggcagtg cggtgcacag
     2461 cgggcgaaga tccaggcctg gccgggcgcc gaaccgcaat tgccgttgcg cgcccgcggt
     2521 ggtcagctca gcggggctgc cctgggccac aacccggcca tagtcgatga tcaccacctg
     2581 gtcggccagc gcttcggctt cctccatcag
//
LOCUS       DATADN010000683         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_394270, whole genome shotgun sequence.
ACCESSION   DATADN010000683 DATADN010000000
VERSION     DATADN010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394270"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1192
                     /locus_tag="VHH53_08830"
     CDS             65..1192
                     /locus_tag="VHH53_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX2290281.1"
                     /translation="MGHRGRREYTNRIRLGDRALPVVSPARFYVCGITPYDVTHLGHA
                     STFVWADAAAAVLRLAGAETVTCRNVTDVDDVLTRAAVQRGRHFDEFALEQEYLFGRD
                     MTALGVRRPEHEPRARHHVGRVVQLASALLATNRAYQRDGAVYFRGADVPAKAGLSLD
                     YAAELAAEFNDPSDEHLCDDPFDVPVWRPSAYDQPGWPSPWGRGRPGWHAECAAIALG
                     VLGPAVDILAGGADLTFPHHAYQTAMVEAATGVAPFARTEFPVGTVTVGGAKMAKSAG
                     NLVLVGNLLTHHLAAAVRLLLLNRRWHQTWDFRAGDIDAEAAHLDRLYSAAGTARTST
                     AASDAVVAALCNDLDVPAALAIGEESGGDAARLVLRTLALE"
     gene            1212..>1516
                     /locus_tag="VHH53_08835"
     CDS             1212..>1516
                     /locus_tag="VHH53_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2290282.1"
                     /translation="MTRTLILLRHAKSAWPDGVPDVQRPLSGRGRRDAPAAGRWLREK
                     FAKIDLVLCSPAVRAAQTWDLASAQLDAIPRVSHDNRLYGASAGELLRITEELPPTAS
                     "
BASE COUNT          243 a          516 c          513 g          244 t
ORIGIN      
        1 cctgggcacg ccttctgagc gagggcagcg gtcggatcgg gacagatgcg ggcaggatgg
       61 aggcatgggt caccgcggtc gacgtgagta caccaacaga atccggctgg gtgaccgcgc
      121 gctgccggtc gtcagcccgg caaggtttta cgtctgcggc atcaccccct acgacgtgac
      181 ccatctgggg catgccagca cgttcgtgtg ggcggacgcg gccgctgcgg tgctacgcct
      241 ggccggagcc gagactgtga cctgccgcaa cgtcaccgac gtcgacgacg tgctgacccg
      301 tgccgccgtg caacgcggtc ggcacttcga cgagttcgct ttggagcagg agtacctgtt
      361 cggccgagat atgaccgcgc tgggggtgcg ccggcccgaa catgagccgc gagcccggca
      421 ccatgtcgga cgggtggtac agctggcctc ggcgctgctt gccacaaacc gcgcctacca
      481 gcgtgacggc gcggtctact tccgtggtgc tgatgtcccc gccaaggccg ggctgagcct
      541 ggattacgca gcggaactcg cggccgagtt caacgatcca tccgatgagc atttgtgcga
      601 cgatccgttc gacgtcccgg tatggcggcc gtcggcctat gaccagccgg gctggcccag
      661 tccgtggggt cgcggccgac ccggctggca cgcggagtgc gccgccatag cgttgggcgt
      721 gctaggcccc gcagtcgaca tcctggcagg tggggccgat ctcaccttcc cgcaccacgc
      781 ctaccagacg gccatggtgg aggcggcgac cggtgtcgcc cccttcgcgc ggaccgagtt
      841 cccggtcggg accgtcacag tgggcggtgc gaagatggcc aagtcggcgg gcaacctggt
      901 actggtcggg aacctgctca cacaccatct agctgcagcg gtgcggttgc tcctgctcaa
      961 ccggaggtgg catcaaacct gggacttccg cgccggcgac atcgatgccg aggccgccca
     1021 tctcgaccgc ctgtactccg cagcggggac agcgcggacc tcgacggccg catcggacgc
     1081 cgtcgtcgct gccctgtgca acgacctcga cgttccagcc gccctcgcga tcggcgaaga
     1141 gtcgggtggt gacgcggcac ggttggtgct gcgcaccctc gccttggaat gagcatcgag
     1201 aggccgcaac tgtgacacgc acgctgatcc tcctcaggca tgcgaaatcg gcatggcctg
     1261 acggtgttcc cgacgtacaa cggccgctgt ccggacgtgg gcgccgcgac gctcccgctg
     1321 ccggccgctg gctgcgagag aagttcgcga agattgatct cgtgttgtgc tcccccgcag
     1381 tgcgagcggc ccaaacctgg gaccttgcct ccgcacaact ggatgcgata ccccgagtca
     1441 gccacgacaa ccggctctac ggcgcctcag ccggggaact cctgagaatc accgaggaat
     1501 tgcctcccac ggcgtc
//
LOCUS       DATADN010000684         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_540839, whole genome shotgun sequence.
ACCESSION   DATADN010000684 DATADN010000000
VERSION     DATADN010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540839"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /gene="ettA"
                     /locus_tag="VHH53_08840"
     CDS             <1..1499
                     /gene="ettA"
                     /locus_tag="VHH53_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX2290283.1"
                     /translation="EAFLAPGASVGILQQEPTLNEDKSVLGNVQEGLGDIKTKLDRFN
                     EIAEKMATDYSDELMEEMGQLQEELDHADAWDLDSQLEQAMDALRCQPPDEPVTHLSG
                     GERRRVALCKLLLSKPDLLLLDEPTNHLDAESVLWLEQFLARYPGAVLAVTHDRYFLD
                     NVAQWILELDRGRAYPYEGNYSTYLEKKADRLAVQGRKDQKLQKRLRDELAWVRSGAK
                     ARQAKSRARLQRYEEMAVEAERSRKLDFEEIQIPAGPRLGNVVVEVSHLDKGFDGRLL
                     IKDLSFTLPRNGIVGVIGPNGVGKTTLFKTIVGLEEPDSGTVKVGETVKLSYVDQERA
                     GIDPNKTVFEVVSDGLDYIQVGNTEMPSRAYVSAFGFKGPDQQKPAGVLSGGERNRLN
                     LALTLKQGGNLILLDEPTNDLDVETLSSLENALEQFPGCAVVISHDRWFLDRVVTHLL
                     SWEGTEENPASWFWFEGNFEAYEQHKVERLGAEAARPHRVTYRRLTRD"
     gene            1547..>2304
                     /locus_tag="VHH53_08845"
     CDS             1547..>2304
                     /locus_tag="VHH53_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEX2290284.1"
                     /translation="MSSTARDGVRADMPVSNGEAASLVESAMLLRWRAPELALLLADR
                     AVAAAQDDQMAVLRADYLAVFALNRLGRHGEAAQRLFPAVWDDGTPAGLRHELQMELA
                     HCAAALAEPATALRAVHAVLAAGDDVAPVLRGTALVAAAEASDALGRGDLFTPALEEA
                     DELYREDSTLDRDTALLLRATARAADAARHRRHGAAAEAEAHARAGRELLAGLADPEH
                     DSGEVRARLMLEIVLAMLDRDEGDAAMHEVRPLLR"
BASE COUNT          420 a          692 c          819 g          373 t
ORIGIN      
        1 gggaggcgtt cctggccccg ggggcgtcgg tgggcatctt gcaacaagag cccacgctga
       61 acgaggacaa gtccgtcctg ggcaacgttc aggaggggct gggagacatc aagacgaagc
      121 tcgatcggtt caacgagatc gccgagaaga tggccaccga ctactccgac gagctcatgg
      181 aggagatggg tcagctgcag gaggagctcg atcacgccga tgcgtgggat ctcgactccc
      241 agcttgagca ggccatggac gccctgcgtt gccagccgcc cgacgagccg gtgacgcacc
      301 tgtccggagg agagcggcgt cgggtcgcgc tgtgcaagct gctgctgtcc aaacctgatc
      361 tgctgttgtt ggacgagccc accaaccacc tggatgcaga gagcgtgctg tggttggagc
      421 agttcctggc gcgctacccc ggtgccgtac tggctgtcac ccacgaccgg tacttcttgg
      481 acaacgtcgc gcagtggatc ctcgagctag accgcggccg ggcctacccc tatgaaggca
      541 actactccac ctacctggag aagaaggccg accgtttggc ggtgcagggc aggaaggacc
      601 agaagctgca gaagcggctg cgcgacgagc tcgcgtgggt gcgatcgggg gcaaaagccc
      661 ggcaggccaa atctagagcc cgcctgcagc gttatgagga gatggcagtc gaagccgagc
      721 gcagccgcaa gctcgacttc gaggagatcc agatcccagc gggacccagg ctgggcaacg
      781 tggtggtgga ggtgtcgcac ctggataagg ggttcgacgg tcgtctgctg atcaaagatc
      841 tgtccttcac gttgccccgc aacggcatcg tgggggtgat cggtcctaat ggggtcggca
      901 agaccacgct gttcaagacc atcgtgggct tggaggagcc tgactccggc acggtcaagg
      961 tgggcgagac ggtgaagctg tcatacgtcg accaggaacg tgcgggcatt gacccgaaca
     1021 agacggtctt cgaggttgtc tccgacggac tggattacat ccaggtcggt aataccgaga
     1081 tgccgtcgag ggcctacgtc agcgccttcg gtttcaaggg ccctgatcag caaaaaccag
     1141 ctggtgtact ctccggcggc gagcgcaacc ggctcaacct ggcgctcacg ctcaaacagg
     1201 gcggcaatct aattctgctc gacgaaccga ccaacgacct cgacgtcgag acgctgagct
     1261 cgctggagaa cgcactggaa cagttccccg gctgtgcagt ggtgatttcc cacgaccgat
     1321 ggtttctgga ccgggtggtg acccaccttc ttagctggga gggaacggag gagaatcccg
     1381 cctcttggtt ctggtttgag ggcaacttcg aggcctacga gcagcacaag gtcgaacgac
     1441 ttggtgcgga ggcggcgcga ccacaccgtg tgacctaccg cagactgacc cgggattgac
     1501 cgggcatggg gcagccgatg atcgggaacg gcgatggggg tgggaggtga gcagtacggc
     1561 gcgcgacggt gtccgggcgg acatgccggt cagcaacggc gaggccgcat cgctggttga
     1621 atcggcgatg ctcttgcgct ggcgagcacc cgagctggct ctgctgctgg ccgaccgggc
     1681 ggtggccgcc gcgcaggatg accagatggc cgttctgcgg gcagattacc tcgcggtttt
     1741 tgctctcaac cgccttggac ggcacgggga agcggctcag cggctgttcc cggcggtctg
     1801 ggacgacgga acccctgccg ggctgcggca tgagctgcaa atggagttgg cgcactgtgc
     1861 ggcggcgctc gccgaaccgg ccaccgcact ccgggcggta cacgcggtgc tggccgccgg
     1921 cgacgatgtt gccccggtgc tgaggggcac tgcactcgtc gccgcagccg aggcgtcgga
     1981 tgcgcttggc cggggagacc tgtttacgcc ggccctcgag gaggctgacg agctctatcg
     2041 ggaggactcc accctcgacc gcgacacagc actgctgttg cgcgccaccg cgcgggctgc
     2101 cgacgcggcg cgccaccgtc gacatggcgc ggctgcagag gccgaggcgc atgccagggc
     2161 aggccgcgag ttgctggccg gtttggccga tcccgagcac gacagcggcg aggtcagggc
     2221 gcggctgatg ctggagattg tgctcgccat gctcgaccgg gacgaaggcg acgccgcgat
     2281 gcacgaggtg cggccgctgc tgcg
//
LOCUS       DATADN010000685         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1147462, whole genome shotgun sequence.
ACCESSION   DATADN010000685 DATADN010000000
VERSION     DATADN010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147462"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..144
                     /locus_tag="VHH53_08850"
                     /pseudo
     CDS             1..144
                     /locus_tag="VHH53_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(150..920)
                     /locus_tag="VHH53_08855"
     CDS             complement(150..920)
                     /locus_tag="VHH53_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2290285.1"
                     /translation="MTGLSNEQLTELVARVHAAHDSGFASRGRPYALGLFGSVALVVC
                     LLRQNLTQAFAGAIFGVSQATVSRRWDLLRPLIGQVLAECVPDPGEVVGAGTVLVDGT
                     VCPTWDWRHIPDLYSAKAGYPGMNLQIAATLSGRLAAVGPVPVHGARHDAHAFGASGL
                     TTLLADIPTAADLGYVGVEGIEIVPVKRLPSESLTATQAEFNTALSKIRAAVEHAIAH
                     LKTWRMLSEEGGRYRAPIQKYQSMLKAVTGLYFFAAYE"
BASE COUNT          348 a          523 c          600 g          334 t
ORIGIN      
        1 gtggaccacg tgaccgtata tcggtgggtt cagcggttca ccccactgct gatcgacgcg
       61 gcacgaccct gtcggcacgc tccgggagat cggtggttcg tcgatgaaac gtacgtcaag
      121 gtcgctggtc ggtgggtcta cctggaggct tattcataag ctgcgaagaa gtagagtccg
      181 gtgacggctt tgagcatgct ttgatatttt tgtatgggtg ctcggtatcg gccaccttcc
      241 tcgctgagca ttcgccaggt cttgaggtgt gcgatggcgt gttcgacggc ggcgcggatt
      301 ttgctcaaag cggtgttgaa ttcggcttgt gtggcggtaa ggctctcgct ggggagtcgt
      361 ttgaccggga caatctcgat gccctcaacg ccgacatagc ccaagtcggc cgcggtgggg
      421 atgtcggcaa gcagggtggt cagacccgag gcgccaaagg cgtgggcgtc gtgccgtgcg
      481 ccgtggaccg ggaccgggcc gacagcggca aggcgtccgc tcagcgtggc ggcgatctgg
      541 aggttcattc cgggatatcc ggccttagcc gagtacagat cggggatgtg gcgccagtcc
      601 caggtagggc aaaccgtgcc gtccaccagc acggtgcccg cacccacgac ctcgcccggg
      661 tcgggcacgc actcggcgag gacctgcccg atcagcggac gcagcaggtc ccaccgccgg
      721 ctcacggtgg cctgactgac gccaaagatt gcaccggcga aggcctgggt gaggttctgg
      781 cgcaacagac acaccaccag cgccaccgag ccgaacagac ccaacgcata aggacggccg
      841 cgggaggcga acccactgtc atgagcggcg tgtacccggg ccaccaactc agtgagttgc
      901 tcattgctca ggcctgtcat agcctcatac cgcaacggct cctccgtttt catttagctg
      961 tctggccgac atcgaaacaa aacgacagga gccgttgccc ttcaggcatg acacgcgcca
     1021 ttcacagatc gtgcgtaaac tgtgagctga cttcagctga gaaagccgtt ggggtgcccc
     1081 gaaccctaca ctacaaaagg cttatgaatt agcctcccgg tatcacaatt ggcatctttg
     1141 cggcggatgc aagctggata agcagctgcc acaggtgatg tgacggacgt ttaaaattca
     1201 gcatgacgct ggtgtctaga aagatcactt taatgcctta gggtgtttat ctgcaagtca
     1261 tccgacgtaa cgccggagac gctgatcctg gttgcctttg aggtcgggtg tcgtgatacg
     1321 tctggtggat ccatagccat gaagccggag tctacggcag caactgtcta caaggtcgcg
     1381 agatccggcg cagcatcaca tcggcgatga ggtgtcattg gcggtgtcat ggaccgtcga
     1441 ctgcaacgaa gagactctgt gggtgagcat ggccaggaaa gcgccggctc gcagctcagg
     1501 cgtgggcagc gcggcgacga gagcagggtg acgccggtgg ctgtgtcagc actcaggggg
     1561 gtcacagttg gccctgccgg ctgaacctgc ccggagcgtc ggagtcgacg acggagacta
     1621 aagcgcactg tgggcacgtc cggccgcggg gtgatgatcg actcgaccgg cacgccgtgg
     1681 gccgatagca gcgacacgaa caagtgccgg catggctgag acgcccctgc cgtcccggac
     1741 agccgccagc cgctcggcgg tcacggtagc cggccctgcc gggctgccgg cggattcggc
     1801 ctggg
//
LOCUS       DATADN010000686         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_68940, whole genome shotgun sequence.
ACCESSION   DATADN010000686 DATADN010000000
VERSION     DATADN010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68940"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..807)
                     /locus_tag="VHH53_08860"
     CDS             complement(106..807)
                     /locus_tag="VHH53_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2290286.1"
                     /translation="MRDPQATNPVPKGREARWRVAGERSGMLAHTVRRPKNRSSLRLR
                     EPRRLPTALSQPDAAELLASFRTWRDRAIAGLMLYCGLRSVEVLGLHVTDVDIGGRWL
                     KVLGKGQRERRVPLDADVASVIQVYLLAQRPESASGRLFLVAKGPNRGQPLTAAGLGT
                     IFRYHRGLTGVVGGHPHALRHTFGTALAEADLAVMQALLGHAHVDTTARYIHLAPAHV
                     KAEFDAARDRIRAQR"
     gene            complement(740..1186)
                     /locus_tag="VHH53_08865"
     CDS             complement(740..1186)
                     /locus_tag="VHH53_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX2290287.1"
                     /translation="MISESGLRLEQRDGGWALAGPAAPRFGLVDEYLGYLADRNYSPK
                     TVRAYGYDLLAFCRWLVVEEQPLAQVSTEVLLRFLRACREAKVAGRPGPNVVRLSGRR
                     MDQYAATTINRRLAAISGAVHLRRDARPAGDQPGPQGPRGPLAGGR"
     gene            complement(1217..>1567)
                     /locus_tag="VHH53_08870"
     CDS             complement(1217..>1567)
                     /locus_tag="VHH53_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEX2290288.1"
                     /translation="ITEHPTKEGALYCAAVMDAYSRLIVGWSIAEHMRTELVTDALGM
                     AIIRRQPDKQPANEQTILHSDHGSQDTSWTFDQRLHTAGLLASMGTVSDSLLTGQCWC
                     CPSSATRRDVVLAG"
BASE COUNT          269 a          532 c          527 g          239 t
ORIGIN      
        1 cagcgccgga agaacgaccg cgccgcctgg cagtacgcgg tgttgccgcg tccgctgcgg
       61 gccaggtggt ccaggtaggc ggtgtgcagg tcggcgctgg acacgtcagc gctgggcgcg
      121 gatgcggtca cgggcggcat cgaactcggc tttcacatga gcgggagcca gatggatgta
      181 gcgggcggtg gtgtcgacgt gggcatggcc cagcagcgcc tgcatcaccg ccaggtcggc
      241 ctcggccagg gcggtgccga aggtgtggcg cagcgcgtgc ggatgcccgc cgacgacccc
      301 ggtgagccct cggtgatagc gaaagatcgt gcccagccca gccgcggtca acggctggcc
      361 ccggttgggt cccttggcca ccaaaaacag ccggccgctg gccgattcgg gtcgctgggc
      421 caacagatac acctggatca ccgaggcgac gtcggcatcg agtgggacgc ggcgttcccg
      481 ttgtcccttg ccgaggacct tcagccaccg accgccgatg tcgacgtcgg tgacgtgcag
      541 gcccaacacc tccaccgagc gcagcccgca atacagcatc aaccccgcga tcgcccggtc
      601 ccgccaggtg cggaagctgg ccaacagctc agccgcgtcc ggctgggaca gcgccgtggg
      661 cagccggcgg ggttcacgaa gccgcaacga cgagcggttc ttcggtcggc gcacggtgtg
      721 cgcgagcatc ccgctgcgct caccggccac ccgccagcgg gcctcgcggc ccttggggac
      781 cgggttggtc gcctgcgggt cgcgcatcgc ggcgaaggtg aacagcaccc gagatcgcgg
      841 ccaaccgccg gttgatcgtc gtggccgcgt actgatccat ccgtcgcccc gacagccgca
      901 ccacgttcgg ccccggccgg cccgcaacct tggcctcccg gcaagcacgc aaaaaccgca
      961 gcagcacttc cgtgctcacc tgcgccagcg gctgctcctc gaccaccagc cagcggcaaa
     1021 acgccagcag gtcataccca taggcgcgca ccgtcttcgg cgagtagttc cggtcggcca
     1081 gataccccag atactcatcc accaacccga accgcggcgc ggccggcccg gccaacgccc
     1141 agccgccgtc tcgttgttca agccgcagac ccgactcgct gatcacggcg aacagatgta
     1201 ccagcagtga acctattcac cccgcaagaa ccacatcacg gcgtgtcgct gaactgggac
     1261 aacaccaaca ttgaccggtt aagagggagt cgctgacggt gcccatggag gcgagtagcc
     1321 cggcggtgtg caggcgttga tcgaaggtcc aagaagtgtc ttgggaaccg tggtcggagt
     1381 gcagtatcgt ttgctcgttg gcgggttgtt tatcgggttg gcggcggatg attgccatgc
     1441 ccagtgcgtc ggtgaccagt tcggtgcgca tgtgctcggc aatcgaccag ccgacgatca
     1501 gtcgggaata ggcgtccatc accgctgcgc agtacaaggc gccttccttg gtggggtgtt
     1561 cggtgat
//
LOCUS       DATADN010000687         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1278640, whole genome shotgun sequence.
ACCESSION   DATADN010000687 DATADN010000000
VERSION     DATADN010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278640"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VHH53_08875"
     CDS             complement(<1..508)
                     /locus_tag="VHH53_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290289.1"
                     /translation="MKLCEPAPVFKDTDVVLAGDLTRSQLRGPAVRRLFQGVYALRGE
                     PVTHELRCVHAVVRRRDRDSTANNPRPAARSAAAGRRRRSRCGAATRSGDLSQIAALL
                     DGRSDKGIVRALHAIELADPRAESRPESRIRVWLLLDGLHPEPQYWIEDSYGRVARAD
                     LAFPEHKVA"
     gene            complement(692..1486)
                     /gene="dapB"
                     /locus_tag="VHH53_08880"
     CDS             complement(692..1486)
                     /gene="dapB"
                     /locus_tag="VHH53_08880"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX2290290.1"
                     /translation="MTRGEQVCPAVARSAENPIRVGVLGARGRVGTEVCKAVDTADDM
                     ELVAMVDAGDWMFNVADAGAEVVVDFTHPDVVMDNVRFCVDQGIHAVVGTTGFDDTRL
                     AQVREWLVPKPGLGVLIAPNFAIGAILSMRFAAMAARFYESVEVVELHHPGKADAPSG
                     TAARTAALIAAARAEAGMGPPPDATTRCLPEARGADVEGVRVHAIRLSGLVAHQEVLL
                     GTEGETLTIRHDTLDRSSFMPGVLLAIRSILGRPGLTVGLEPLLDL"
     gene            complement(1483..>1816)
                     /locus_tag="VHH53_08885"
     CDS             complement(1483..>1816)
                     /locus_tag="VHH53_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX2290291.1"
                     /translation="VAAVVDEVLGSVARDGLSDAELVRGKGQLRGGLVLGLEDPGSRM
                     TRIGKAELHHGEHRTITEELRRIDAVTADQVTELAREFLRQPVTAAVAGPYAHAQELP
                     GEVTELIA"
BASE COUNT          323 a          648 c          559 g          286 t
ORIGIN      
        1 ctgcgacctt gtgctcgggg aaggccagat cagcccgagc gacgcggccg tacgagtcct
       61 cgatccagta ctgcggctca ggatgcaagc cgtccagcag gagccatacc cggatccgcg
      121 actccggccg cgactcggct cgaggatcgg cgagttcgat ggcgtggagg gcgcgcacga
      181 tgcctttgtc cgaacgtccg tcgagcagcg ctgcaatctg ggacaggtca ccagaccgtg
      241 tcgcagcacc gcatcgagat cggcgacggc gtccggcagc ggctgatcga gcagcaggtc
      301 gagggttgtt cgcagtggag tcgcgatcgc gtcttcggac cacggcgtgc acgcagcgca
      361 actcatgcgt caccggctca ccccggagcg cgtacacacc ctgaaacaac cgccgcactg
      421 ccggcccccg cagctgactg cgggtgaggt cgcccgccag caccacatcg gtgtctttga
      481 aaaccggtgc cggctcacaa agcttcatct gtctcgccat gcgccagact gtgccgctac
      541 tcttcccgct actcaaacgt ccatccacca ggctacttaa gttatccaca gtctcgttcc
      601 ggccatcccg caattcggct ccgaatggcg attactgaat ggccgctacc gcaattgggc
      661 tccgaatggc gccttgggac cagaacaagg gctacaggtc gagcaggggt tccaggccta
      721 ctgtgagacc tggacggccc agtattgagc gaatggcaag gagcacgccg ggcatgaacg
      781 acgagcggtc caacgtgtca tgcctgatcg tcagcgtctc gccctcggtg cccagtaaca
      841 cctcctggtg ggcgaccaac ccagacagcc gtatggcatg cacccgtact ccctcgacgt
      901 cggcaccccg agcttcaggc aggcaacggg tggtggcatc gggtggcggg cccatcccgg
      961 cttcggcccg ggcggccgcg atgagcgccg cggtgcgggc tgcggtcccg gaaggagcat
     1021 cggccttacc agggtggtgc agctccacca cctccaccga ctcgtagaac cgggcggcca
     1081 tggcggcgaa acgcatcgac aggatcgccc cgatggcgaa gttgggagcg atcaggaccc
     1141 cgaggccggg cttgggcacg agccactcgc ggacttgggc caaccttgtg tcatcgaagc
     1201 ccgtggtccc caccacggcg tgaatgccct gatccacgca gaaccgcacg ttgtccatca
     1261 ccacgtcggg gtgggtgaag tcgaccacca cctcggctcc cgcgtcagca acgttgaaca
     1321 tccagtcacc cgcgtcgacc attgcgacga gttccatgtc gtcagcggtg tccacggcct
     1381 tgcacacctc ggtgccgacc cggccacgag ccccgagcac cccgacccgg atcgggttct
     1441 ccgctgaccg cgccacggct gggcagacct gctccccgcg ggtcatgcga tcaactccgt
     1501 aacctcaccg ggcagctcct gcgcatgcgc atagggaccc gccacagcgg cagtgaccgg
     1561 ttggcgcaga aactcgcgag cgagttcagt gacctggtct gcggtcaccg cgtcgatccg
     1621 tcgtagctct tcggtgatgg tgcggtgctc accgtggtgc agctcggcct tgccgatccg
     1681 ggtcatccgg gaacccggat cctccaggcc cagcaccagc ccaccgcgca gctgcccttt
     1741 accccggacc agctcggcgt cggacagacc atcccgggcc accgagccca gcacctcgtc
     1801 caccaccgcc gccacc
//
LOCUS       DATADN010000688         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_394618, whole genome shotgun sequence.
ACCESSION   DATADN010000688 DATADN010000000
VERSION     DATADN010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394618"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..966
                     /locus_tag="VHH53_08890"
     CDS             181..966
                     /locus_tag="VHH53_08890"
                     /inference="COORDINATES: protein motif:HMM:NF025377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX secretion-associated protein EspG"
                     /protein_id="HEX2290292.1"
                     /translation="MMPLAWRLSAAQWDVLTEVLGLEGYPAPIQVRSTGRTRLEHARV
                     RADVCVELSQLGLLRAGRIDAELEAAVCLLHWPTTWVDSVWLADAVADQPVRVIAARG
                     GAAGVCALQHPDQLGATLLEVIPTTGLAAAVVSRLPSHPPGRGCAVTVRPHSAQPAGG
                     LLVAASPVRSSADRDLAAAAAILDQPHVWAGQIAANVRDPSGQVRRSDVLRWCDNVDG
                     RYHVTGGPAIRRSGESTSLTIGPSDPQRLGDEVQRLLDALQPR"
     gene            994..1965
                     /locus_tag="VHH53_08895"
     CDS             994..1965
                     /locus_tag="VHH53_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX2290293.1"
                     /translation="MRSVLRAGGAARGVVLRAVLGIMVGALCISGCSSGHSQVGAAAL
                     VNGTEIPVEQVQSQLTMVLTKEGDQARAQLAADRQLDDVSRKIITVQIRHELTAIAAQ
                     RAGLTVDQARVNRLISEVGGAQGASKGTIFDAAGYRQRAVDKLLTAQLGLGALRNSAV
                     TVDYTTADTRTAAKAKVDELSRAGAKRARQLIDADVRAGKDAVIGKRIVAADDPIFAT
                     TPAFGVDEGTVVAFQLADTTPWLIMVIKNRANRGVQPSDNAPALEQIDPAVLEAIGLR
                     QLAQVGEEVEVRLSPRYGIWDPVSLQAVANENETAGFLAPLRATPRT"
BASE COUNT          316 a          672 c          730 g          314 t
ORIGIN      
        1 gggtgggcga gccggcctgg gggatgtgat atcaccggtc ggcggcggac gtggcaacgg
       61 cggtgaggac accgagcacc ggcggccttc ctacctgatc gagatgggag acgtcttcac
      121 cgacgggcgc aaggtcgctc caccggtgat cggcgaggaa ccgctcgagc gcgggtaatg
      181 atgatgccct tggcatggcg gctgtccgcc gcacaatggg acgtgctcac cgaggtgctg
      241 ggtcttgagg gctaccccgc gccgatccag gtgcgctcga ccgggcgcac ccgcctcgag
      301 cacgcgcggg tccgcgccga cgtgtgcgtc gagctgagtc agctcggcct gctccgtgct
      361 ggtcggatag atgctgagct ggaggctgca gtgtgcctgc tgcactggcc gaccacctgg
      421 gtggactcgg tatggcttgc tgacgcggtg gctgatcagc cagttcgggt gatcgcggct
      481 cggggtggcg ccgcaggcgt ctgcgcgctg cagcatcccg atcagcttgg cgccacgttg
      541 ctggaggtga ttccgacgac gggcctggct gcagcggtgg tgagccggct cccgtcgcat
      601 ccgccgggcc gcggctgcgc ggtcaccgtg cgaccgcata gcgcgcagcc cgcgggcggg
      661 ttgcttgtcg ccgcgtcccc ggtgcgcagc agtgccgacc gtgaccttgc cgccgctgcc
      721 gcgatcctgg accaaccgca cgtgtgggcc gggcagatcg ccgccaatgt gcgagatccg
      781 tcgggccagg tgcgtcgttc ggacgtactg cgctggtgcg acaacgtcga cggccgctac
      841 cacgtgaccg gcggtcccgc gatccggcga tccggcgagt cgacgtcgct gaccatcggg
      901 ccgtcagatc cacaacgact cggcgacgaa gtacagcggc ttctggacgc cctccaaccg
      961 cgctgaatgg ggccgcgtga gaaggtagcg cccgtgcgaa gcgtcctgcg tgccggcggt
     1021 gcagcgcgtg gtgtcgtgct cagggccgtc ctcgggatca tggtaggagc actgtgcatc
     1081 agtggctgca gctccggcca cagccaggtc ggtgccgccg cgttggtcaa cggtaccgag
     1141 atcccggtgg agcaggtgca gagccagctc accatggtgc tgactaagga aggtgaccag
     1201 gcccgggccc aactggcggc tgaccgccag ctcgatgacg tatcacgtaa gatcatcact
     1261 gtccagatca ggcacgagtt gacggcgatc gccgcgcagc gcgcaggcct gacggtggat
     1321 caggcccggg tgaacaggct gatcagcgaa gtcggtggtg ctcagggcgc ttccaaggga
     1381 acgatcttcg atgcagccgg gtaccggcag cgggccgtcg acaagctgct cacggcccag
     1441 ctcgggctgg gcgccctgcg taacagcgcg gtcaccgtcg actacaccac ggccgacacc
     1501 cgaaccgccg cgaaggccaa ggtggacgag ctgtcccggg cgggagccaa gcgcgcccgg
     1561 cagctgatcg acgccgacgt tcgggccggc aaggacgccg tgatcggcaa gcggatcgtc
     1621 gctgctgacg atccgatctt cgccaccact ccggcattcg gggtcgacga gggaaccgtg
     1681 gtggccttcc agctcgcgga caccacgccc tggctgatca tggtgatcaa gaaccgcgcc
     1741 aaccgcggtg tccagccatc cgacaacgcg cccgcactcg aacagatcga tccggccgtg
     1801 ctcgaagcca tcggcttgcg ccagctggcc caggtggggg aggaggtgga agtccgactg
     1861 agcccccgtt acggcatatg ggacccggtg agcctgcagg cggtggccaa cgagaacgag
     1921 accgccggct tcctcgcgcc gttacgcgca actccccgga cgtgaccgcc gctccgacat
     1981 cggtcgttgt tgcgctgtct ccacggctcg gcgcggtgtt tccggccgcg gc
//
LOCUS       DATADN010000689         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_69548, whole genome shotgun sequence.
ACCESSION   DATADN010000689 DATADN010000000
VERSION     DATADN010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69548"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..482)
                     /locus_tag="VHH53_08900"
     CDS             complement(210..482)
                     /locus_tag="VHH53_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX2290294.1"
                     /translation="MTRAVRLAAAAAVTLAVAGGMGWMGQAASPGLPVETAQTRVGAG
                     ETVWDVAQRVAPNSDRRAVVEKIRQLNGMADSAIQPGQQLQVPDEE"
     gene            937..1698
                     /gene="lexA"
                     /locus_tag="VHH53_08905"
     CDS             937..1698
                     /gene="lexA"
                     /locus_tag="VHH53_08905"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX2290295.1"
                     /translation="MGRQRSDEPEGAGPDAQPPGADDGAQVHAFPDLDDSAGDDGLTP
                     RQRRVLEVIRDWVQRCGYPPSVREIGEAVGLTSTSSVAHQLRALERKGYLRRDPNRPR
                     AVGVRPAESALDTLGVSGVSGGSSARPTPTFVPVVGRIAAGGPVLAEQAIEDVFPLPK
                     EIVGEGSLFLVRVAGDSMIDAAITDGDWVVIRQQPTAENGDIVAAMIDGEATVKTFKR
                     RDGHVWLLPHNPVYQPIPGDEATVLGRVVAVLRRL"
     gene            complement(1747..3174)
                     /gene="hflX"
                     /locus_tag="VHH53_08910"
     CDS             complement(1747..3174)
                     /gene="hflX"
                     /locus_tag="VHH53_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX2290296.1"
                     /translation="MTTLPIFPPHDPGPASRGELDSEDRSALRRVAGLSTELADVTEV
                     EYRQLRLERVVLVGVWTDGSAAEADAALAELARLADTAGAQVLDGLIQRRQRPDPATY
                     VGSGKVMELREIVLRTAADTVICDGELTPGQLRQLEAKLKVKVIDRTALILDIFAQHA
                     SSREGKAQVELAQLSYLLPRLRGWGESLSRQAGGAGGGASGGVGTRGPGETKLETDRR
                     RIHRRLAKLRREIAGMRTVRDTKRSRRRANEVPNVAIVGYTNAGKSSLLNAITGAEVL
                     VQDALFATLDPTTRRAVTPDGRDYTLTDTVGFVRHLPHQLVEAFRSTLEETADADLLL
                     HVVDGSDPMPERQVEAVREVLAEITEGRELPMPPELLVVNKIDDADQFVLSRLRHLLP
                     DASFVSAHTGAGITGMVGRITDALPRIDVEVDALLPWTQGSLVARVHAEGEVLDTAHT
                     EAGTRLRARVRPDLAGALAGYLVDA"
     gene            complement(3271..>3790)
                     /gene="dapF"
                     /locus_tag="VHH53_08915"
     CDS             complement(3271..>3790)
                     /gene="dapF"
                     /locus_tag="VHH53_08915"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX2290297.1"
                     /translation="FRIGSRSGPRAVRCNDDGSVAVEMGRVRRTGTSTVTVEGAAFNG
                     LAVDVGNPHLACLTEVPVEQLDLTVPPGHDPGIFPHGVNVEFVNVLCAGHLRMRVHER
                     GVGETRSCGTGTVAALAAALDAQGRDTGEADVQITGGVVHVTISEDGSTLTGPAVLVA
                     QGRLTLDWWLAH"
BASE COUNT          638 a         1292 c         1241 g          619 t
ORIGIN      
        1 cggacaacgc atgtaccccc tccccaaccc cacttgtggg caaactacag ccttgtaact
       61 actatatgtg ggggcagaca gtaagtcgag ccgccacgac ggccgggttc taccgcgacc
      121 gtgaggtgtg acgctctcat ggcgtacgcc agcagtgacc acggggccag tggccaagca
      181 cagcgccggc gaagtcggca gaccccgacc tactcctcgt cgggcacctg caactgctgg
      241 ccgggctgga tggccgagtc tgccatgccg ttcagttgcc gaatcttctc caccaccgca
      301 cgtcggtcag agttcggcgc cacccgctgc gcaacatccc acacggtctc gccagcgccg
      361 acccgggtct gtgcggtctc aaccggcaac ccgggactgg cggcctggcc catccaaccc
      421 atcccgccgg ccacagctaa tgtcactgcg gcggcggccg ccagacgcac tgccctggtc
      481 actcttggcg ctgcgatgcg tgcctccggg gccccgcatc ccaacgggtg ccgcaaccgc
      541 cgacgcacca gaggccgttg cgccggagga gcgagaggga gcacgggagg tggaggtgcc
      601 agaagccgcc tggtgggcat cgtttcgtac ggcggcttga cgcccaccgg tccacttgcc
      661 gccagcccga actttgaatc gcgcgccgcg ggccggttgc tcaactgcgc tggtgcggtc
      721 atcgagactc ctatcgcttg gtagccgctt agtgccgcct cattgcatcg aacacgggtt
      781 cgatcgaact gctgtgcggt gtctatcacg acggtccgac agaatcgacg actacgcggc
      841 gtgttgttcg aacagatgtt tgatctctac tgagacgccg gttaacctcg actccagagc
      901 ggaccgatct ggtgtgtgtt gctgtgagga ggaccggtgg gacgacagcg gagcgacgaa
      961 ccggaaggcg ccggtcccga cgcgcagcca cccggcgcgg acgatggcgc gcaggtacat
     1021 gcctttcctg acctcgacga cagcgctggt gacgacggcc tcaccccacg gcaacgacgg
     1081 gtccttgagg tgatccgaga ctgggtgcag cgctgcggtt accccccgag tgtgcgggag
     1141 atcggcgagg cagtgggact cacatccacc tcgtcggtcg cacaccagct acgcgcgttg
     1201 gagcgtaagg ggtacttacg ccgcgacccc aaccgaccgc gcgcagtcgg cgtccgacct
     1261 gcggaaagcg cactcgacac cttgggggtt tcgggggtct cggggggttc cagcgcccgt
     1321 cccactccga ctttcgtgcc cgtggtcggt cggatcgcgg caggcggtcc ggtgctcgcc
     1381 gagcaagcca tcgaagatgt ctttccctta ccgaaggaga tcgtcgggga gggttcgctg
     1441 ttcctggtga gggtcgcagg tgattccatg atcgacgcgg cgatcaccga cggggactgg
     1501 gtggtcatcc gccagcagcc caccgccgag aacggggaca tcgtcgctgc catgattgac
     1561 ggcgaggcga ccgtgaagac gttcaagcgc cgtgacgggc atgtctggct gctcccgcac
     1621 aaccctgtct accagccgat ccccggggac gaagcgaccg tgctcggccg ggtggtggcg
     1681 gtgctgagac ggctgtgagc cggctggctc acagctaccg gtccctgcgt ctgcgtaacc
     1741 gacggatcag gcgtccacca ggtacccggc caacgcgccg gcaaggtcag gccgtacccg
     1801 ggcacgcagc cgggtaccgg cctccgtgtg ggcggtgtcc agcacctccc cctcggcgtg
     1861 cactcgggcc accagagagc cttgggtcca gggcagcagt gcgtcgacct caacatcgat
     1921 tcggggcaac gcatcggtga ttcgcccgac catgccggtg atgccggcgc ccgtgtgcgc
     1981 agagacgaac gacgcgtccg gtagcaggtg acgcagccgc gacaacacga actggtcggc
     2041 atcatcgatc ttgttcacga ccagcaactc cggtggcatc ggcaactcgc gaccctcggt
     2101 gatctcggcg agtacctcgc ggaccgcctc cacctgccgt tcgggcattg ggtcagaccc
     2161 gtccaccaca tgcaacagca ggtcagcgtc ggccgtctcc tccaacgtgg agcgaaacgc
     2221 ctccaccaac tgatgcggca ggtggcggac gaaaccgacg gtgtccgtga gcgtgtagtc
     2281 gcgtccgtct ggggtgacag cccggcgtgt ggtggggtcc aaggtcgcga acagggcatc
     2341 ctgtaccagc acctcggcgc cggtgatcgc gttgagcaag ctggacttgc ccgcgttggt
     2401 gtatccgacg atggccacgt tgggcacctc gttggcgcgc ctgcggctgc gcttggtgtc
     2461 gcgaaccgta cgcatcccgg cgatctcgcg gcgcagtttg gcgagccggc ggtgaatgcg
     2521 ccgccggtcg gtctccagct tggtctcacc cggtccacgg gtgccgactc caccactggc
     2581 tccgccgcca gcaccaccag cctgccggga cagcgactca ccccagccgc gcagccgcgg
     2641 caataagtag ctcagctggg ccagctcgac ttgtgccttg ccttcccggg agctggcgtg
     2701 ctgggcgaag atgtcgagga tcagcgcagt ccggtcgatg accttcacct tgagcttggc
     2761 ctccagctgc cgcagctggc cgggagtgag ttcgccatcg cagatcaccg tgtcggcagc
     2821 ggtgcgcagg acgatctcgc gcagctccat caccttgcca gagccgacgt aggtcgccgg
     2881 gtcgggacgt tggcggcgct ggatgaggcc atcgagcacc tgcgctccag cagtgtcggc
     2941 aagtcgggcc agctcggcca acgccgcatc ggcctcggcc gcagagccat cggtccacac
     3001 cccgacgagg accacgcgct ccaagcgcag ttgccggtac tcgacctcgg tgacatcagc
     3061 gagttcggtg gacaggcctg ccacgcggcg caacgcggaa cggtcctcgc tgtccagctc
     3121 acctcgggag gcagggccgg gatcatgtgg cgggaagatc ggtaaggttg tcatacgcct
     3181 ctcatcgtga cacgtcggat gaagcacgcc cacccgaatt cgggtgccat gcagtccggg
     3241 tccaggttgc gctgaacgac ggtggggcag tcagtgcgcc aaccaccagt cgagagtcag
     3301 ccggccttgg gcgacaagca ccgcgggacc agtcagtgtg ctgccatcct cggagatcgt
     3361 tacatgaact actccgccag taatctgcac gtcagcctcg ccggtgtccc gaccctgggc
     3421 atccagcgcg gctgcaagcg cagccactgt tccggtgcca caggagcggg tctcccccac
     3481 tccgcgctcg tgcactcgca tccgcagatg accagcgcac agcacgttga caaattcaac
     3541 gttgacgccg tgcggaaaga ttccggggtc gtgccccggc ggcacggtga ggtcgagctg
     3601 ctccaccgga acctcggtca ggcaggccag atgcgggtta ccgacgtcca ccgcgagtcc
     3661 gttgaatgcc gcgccctcaa cggtcaccgt ggaggtgccg gtgcgtcgca ccctccccat
     3721 ctcgacggcc actgagccat cgtcgttgca ccgcaccgcg cgtggaccgg acctgctgcc
     3781 gatccggaac
//
LOCUS       DATADN010000690         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_998071, whole genome shotgun sequence.
ACCESSION   DATADN010000690 DATADN010000000
VERSION     DATADN010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998071"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1444)
                     /locus_tag="VHH53_08920"
     CDS             complement(167..1444)
                     /locus_tag="VHH53_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEX2290298.1"
                     /translation="MRSPNRLIGVTAVAVTALAIVTSPASAHDPNSPEGKAEAAAMER
                     DHEPAWRTDPIAGMALVPCTNGMAGQFPCRNVDLLAHLPLSSIGGGQGNSMWGWTDTA
                     TGREYIIFGRSSGAAFVDVTTPTSPRYLGNLPSHNGTSSSWRDIKVYRNHAFIGADGI
                     SGHGMQVFDLTRLRNVTTPQTFTPNTRYSGFGNSHTLSVNEETGFIYAVGTNTCSGGV
                     HMVNVQTPTSPRNAGCVSNDGYVHENQCVIYRGPDTTHRGREICFNYNEDTLTIVDVT
                     TKTSPRQLSRTSYANVGYTHQGWLTGDHARLVMDDELDSNPRTLIWNVSDLDRPVHTG
                     TYNPTFGSAIDHNQYIVGNRVYQANYRAGLRILDASAIASNRLTEIAFFDIYPANNSS
                     GFNGAWNVYPFFASGTIAISGIEQGLVLVRATV"
BASE COUNT          242 a          498 c          589 g          284 t
ORIGIN      
        1 gaacacaacc acggcgacca gcagcagggc ggcgacggcc agcacgttcg tcaccgcccc
       61 gcgcggcccg tgaccgccga gttgggcatg cagttccgcc agcctggcgg tccggtcgcc
      121 gatcaccatg gcggctcagc agggggccac tggtcgtcgg ccacggctac accgtcgctc
      181 ggaccagcac cagaccctgc tcgattccgc tgatggcaat ggttccgctg gcgaagaacg
      241 ggtagacgtt ccacgccccg ttgaacccgc tgctgttgtt ggccgggtag atgtcgaaga
      301 aggcgatctc cgtcagccgg ttggacgcga tcgcgctggc atccaggatg cgcaacccgg
      361 cccggtagtt ggcctggtag acgcggttgc ccacgatgta ctggttgtgg tcgatggcgc
      421 tgccgaaggt cgggttgtag gtgccggtgt gcaccgggcg gtccaggtcg gacacgttcc
      481 agatcagcgt ccgcgggttg ctgtccagct cgtcgtccat gaccaggcgg gcgtggtcac
      541 cggtcagcca gccctggtgg gtgtagccca cgttggcgta gctggtgcgg gacagctgcc
      601 gcggcgaggt cttggtggtg acgtcgacga tggtcagcgt gtcctcgttg tagttgaagc
      661 agatctcccg gccccggtgg gtggtgtccg ggccgcggta gatcacgcac tggttctcgt
      721 ggacgtagcc gtcgttgctg acacagccgg cgttgcgcgg cgaggtcggg gtctgcacgt
      781 tgaccatgtg cacgccaccg ctgcaggtgt tggtgccgac cgcgtagatg aagccggtct
      841 cctcgttgac cgacagcgtg tgcgagttgc cgaagccgga gtagcgggtg ttgggggtga
      901 acgtctgcgg cgtggtgacg ttgcgcagcc gggtcaggtc gaacacctgc atgccgtggc
      961 cggagatgcc gtcggcgccg atgaaggcgt ggttgcggta gaccttgatg tcccgccagc
     1021 tgctgctggt tccgttgtgc gacggcaggt tgcccaggta gcgcgggctg gtcggagtgg
     1081 tcacgtcgac gaacgcggcg ccgctactgc ggccgaagat gatgtactcc cggccggtcg
     1141 ccgtgtcggt ccaaccccac atgctgttgc cctggccgcc accgatgctg ctcagcggca
     1201 ggtgggccag taggtcgacg ttgcggcacg ggaactggcc ggccatgccg ttggtgcagg
     1261 gaacgagggc catgccggcg atcgggtcgg ttcgccaggc cggctcgtgg tcccgctcca
     1321 tggcggctgc ctcggcctta ccttccgggg aattgggatc gtgggccgac gccggcgatg
     1381 tgacgatcgc cagggcggtg accgcgaccg cggtgacgcc gatcagtcgg ttggggctgc
     1441 gcatagtcac tcctcgacat cgcgccaaca gggagacacg acgaaacggg ctgtccgccc
     1501 acaaccacgg taagtgcctg ccccgcagcc gaacccatac caagagacca tgtcaagggt
     1561 tgatggccag gtggatcacc ccgcagcggc agcggcgccg gtaggccaga atg
//
LOCUS       DATADN010000691         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1279604, whole genome shotgun sequence.
ACCESSION   DATADN010000691 DATADN010000000
VERSION     DATADN010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279604"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VHH53_08925"
     CDS             complement(<1..1500)
                     /locus_tag="VHH53_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX2290299.1"
                     /translation="MPSATRRVRARLARRITAQRPGPVRQVLEPLAVVHRELHPKADL
                     ALLQRAYDVAEAKHSQQQRKSGDPYITHPLAVSTILAELGMDTTTLVAALLHDTVEDT
                     DYSLSRLTQDFGDEVAHLVDGVTKLDKVKLGAAAEAETIRKMVIAMARDPRVLVIKLA
                     DRLHNMRTMRFLPPEQQARKAKETLEVLAPLAHRLGMATIKWELEDLSFAILHPKKYD
                     EIVRLVADRAPSRDTYLRTVIDEVSAQLAGSRITATVEGRPKHYYSIYQKMIVRGRDF
                     DDIHDLVGVRIMVEDVRDCYAAVGVVHSVWQPMPGRFKDYVAQPRFGVYQSLHTTVIG
                     PDGKPLEVQIRTRDMHRTAEYGIAAHWRYKETRGTHNGAVDVDEMAWMRQLLDWQREA
                     ADPGEFLESLRYDLATREIFVFTPKGDVITLPAGSTPVDFAYAVHTEVGNRCIGARVN
                     GRLVALERQLENGEVVEIFTSKAEGAGPSWDWLSFVASPRARAKIRQWFA"
     gene            complement(1681..>1936)
                     /locus_tag="VHH53_08930"
     CDS             complement(1681..>1936)
                     /locus_tag="VHH53_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX2290300.1"
                     /translation="CDSEAYALEYGVASLELAVGALRPGQRALVIDDVLATGGTVAAT
                     CALVERAGASVSGIAVLLEIGALQGRHRLGGRVLHALLTI"
BASE COUNT          320 a          653 c          637 g          326 t
ORIGIN      
        1 ggcgaaccac tgcctgatct tcgcccgggc ccgtggtgag gcgacgaagg acaaccagtc
       61 ccagctgggg ccggccccct cggccttcga ggtgaagatc tcgacgacct cgccgttctc
      121 caactgccgc tccaacgcga caagccggcc gttgacccgc gcgccgatgc accgattacc
      181 gacctcggtg tgcaccgcgt aggcgaagtc gaccggcgtg gaacctgcgg gcagcgtgat
      241 gacgtcgccc ttgggcgtga agacgaagat ctcgcgggtg gcgaggtcat agcgcagcga
      301 ctcgagaaac tcaccggggt cggcggcctc gcgctgccag tccagcaact ggcgcatcca
      361 ggccatctca tcgacgtcca cggctccgtt gtgggtgccg cgggtctcct tgtaccgcca
      421 gtgcgcggcg atgccgtact ccgcggttcg gtgcatatcg cgggtgcgga tttgcacttc
      481 caacggtttg ccgtccggcc cgatcacggt ggtgtgcagc gactggtaga ccccgaaccg
      541 gggctgcgcg acgtagtcct tgaagcgacc gggcatcggc tgccacaccg agtgcaccac
      601 gccgaccgcg gcatagcagt cgcgcacgtc ttcgaccatg atccgtacgc cgaccaggtc
      661 gtggatatcg tcgaaatcgc gaccgcgcac gatcatcttc tggtagatcg agtagtagtg
      721 cttgggcctg ccctcgacgg tagcggtgat ccgcgaaccg gccagctgcg cggagacctc
      781 atcgatcacc gtccgcaagt acgtgtcgcg tgatggcgcc cgatcagcca ccagccgaac
      841 gatctcgtcg tacttcttcg gatgcaagat cgcgaaggaa aggtcttcca gttcccactt
      901 gatggtggcc atgcccagcc ggtgagccag cggcgcgagt acctcgaggg tctccttggc
      961 cttgcgggct tgttgctcgg gcggcaggaa ccgcatggtg cgcatgttgt gtaatcggtc
     1021 cgccagcttg atcaccagca cccgcgggtc gcgcgccatc gcgatgacca tcttgcggat
     1081 tgtctcggcc tcggcggcgg cgccgagttt gaccttgtcg agcttggtga caccgtcgac
     1141 gaggtgcgca acctcatcac cgaaatcctg ggtgagacgg gacagcgaat agtcagtgtc
     1201 ctcgacggtg tcatggagca gcgcggccac cagcgtcgtg gtgtccatcc ccaactcggc
     1261 gaggatcgtc gagaccgcca acggatgggt gatgtacggg tcgcccgatt ttcgttgctg
     1321 ctgggagtgc ttggcctcgg caacgtcgta agcccgttgc agcaacgcga ggtcagcctt
     1381 gggatgcagc tcccggtgca ccacagcgag tggttcgagc acctgcctga ccggacccgg
     1441 ccgctgcgcg gtgatccgcc gcgcgagccg ggcccgaacc cgccgggtcg ccgagggcag
     1501 ccgcgcagcg accgtggccg aatctggagc tgggcctggg tctgggccgg caggcggcgc
     1561 agcggtgcgc tgcagatcct ggctcaagcg cactctcccg ccgcgtggtg accgatcggc
     1621 tttcagggta ccgccgcgtg ccggcgcgag atccgcagga ccgggagatc tcggtacttg
     1681 tcagatcgtc agtagagcgt gcagcacgcg gccgccgagc cggtgacggc cctgaagcgc
     1741 accgatttcc agcagcaccg caatgccact caccgaggcc ccagcccgct cgaccaatgc
     1801 acaggtcgcg gctaccgtcc ccccggtggc cagcacatcg tcgatcacca gtgcccgctg
     1861 tcccggccgc agcgcgccga cagccagttc cagcgaggcg acgccgtact ccagagcata
     1921 agcctcactg tcgcag
//
LOCUS       DATADN010000692         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_226686, whole genome shotgun sequence.
ACCESSION   DATADN010000692 DATADN010000000
VERSION     DATADN010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226686"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /gene="rpsK"
                     /locus_tag="VHH53_08935"
     CDS             complement(<1..274)
                     /gene="rpsK"
                     /locus_tag="VHH53_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX2290301.1"
                     /translation="MPPRARTGTGVKKVRRKEKKNVAHGHAHIKSTFNNTIVSITDPT
                     GAVIAWASAGHVGFKGSRKSTPFAAQMAAENAARKAAEHGMKKVDVF"
     gene            complement(313..693)
                     /gene="rpsM"
                     /locus_tag="VHH53_08940"
     CDS             complement(313..693)
                     /gene="rpsM"
                     /locus_tag="VHH53_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX2290302.1"
                     /translation="MARLAGVDLPREKRMEIALTYIYGIGRTRSHEILSATGVSPELR
                     SKDLSDEDLVALRDYIENNYRVEGDLRREVQADIRRKIEIGCYEGLRWRRGLPVRGQR
                     TKTNARTRKGPKKTVAGKKKAGKK"
     gene            complement(863..976)
                     /gene="rpmJ"
                     /locus_tag="VHH53_08945"
     CDS             complement(863..976)
                     /gene="rpmJ"
                     /locus_tag="VHH53_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX2290303.1"
                     /translation="MKVQPSVKKICDKCKVIRRHGVVMVICENQRHKQRQG"
     gene            complement(995..1216)
                     /gene="infA"
                     /locus_tag="VHH53_08950"
     CDS             complement(995..1216)
                     /gene="infA"
                     /locus_tag="VHH53_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX2290304.1"
                     /translation="MGKKDGAIEVEGKVVEPLPNAMFRVELENGHRVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLSRGRIVYRYK"
     gene            complement(1449..1925)
                     /locus_tag="VHH53_08955"
     CDS             complement(1449..1925)
                     /locus_tag="VHH53_08955"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX2290305.1"
                     /translation="MDAMTGTMGIVDGSTPRDRMRAADTDRKRVAEQLRSAHSDGRLD
                     LTEYDERLQQAWAARTYGELDALTADLPSARPPVDPANHEVQQSHWQCRHGRGGRAVV
                     AAWASVSVVNLLIWAVVSLATMSWIYPWWVWVAGPWGAVLLAGWIGERARALRPAR"
     gene            complement(2015..3169)
                     /locus_tag="VHH53_08960"
     CDS             complement(2015..3169)
                     /locus_tag="VHH53_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX2290306.1"
                     /translation="MREVLDELVAEWREGKSTAVGTVVSTYRSAPRPAGASMLVTSDE
                     RAVGSVSGGCVEGAVFELGQQVLTDDAPILQRYGVSDDDAFTVGLTCGGIIDVFVEKV
                     DPVHFPELEEVADSVAKKEGVAVATVVAHPDPERVGSRMVVWADRHSGTTGSSSADEA
                     ILEDVRGALAAGRTATLHYGPEGERRGEGMDVFVNAMQPPPRMLVFGAIDFAAAMARI
                     GVFLGYYVTVCDARPVFATKSRFPDAHEVIVNWPHRYLKEEVEAGRIDPRTVITVLTH
                     DPKFDVPVLVEALKLDLAYIGAMGSRRTHKDREERLREAGVTDEELSKIASPIGLDLG
                     ARTPEETAVSIAAELISLRWGGRATQTRLADAEGPIHGASPIAEPEPATT"
BASE COUNT          504 a         1111 c         1015 g          596 t
ORIGIN      
        1 cgaagacgtc gacctttttc ataccgtgct cagcagcctt gcgggccgcg ttctcggcgg
       61 ccatctgcgc ggcgaacggg gtggacttgc gcgagccctt gaagccgacg tgacccgcgg
      121 aggcccaagc gatcaccgca ccagtggggt cggtgatcga gacgatcgtg ttgttgaacg
      181 tcgacttgat gtgcgcatgg ccatgcgcga cgttcttctt ctccttgcgc cgcaccttct
      241 tgacgccggt acctgttcgt gctctgggtg gcatacggtt gcactcctac aaaagtctgg
      301 ggtctacggg ggttacttct tcccggcctt cttcttgccg gcgaccgtct tcttcggacc
      361 cttgcgggtg cgggcgttgg tcttggttcg ctgaccgcgc acgggcagcc cgcggcgcca
      421 ccgcagcccc tcgtagcagc cgatctcgat cttccggcgg atatcggctt ggacctcgcg
      481 gcggaggtct ccctcgactc tgtaattgtt ctcgatgtag tcccgcagcg cgacgaggtc
      541 ctcgtcggac aggtccttgg aacgcagttc agggctcacc ccggtagcgc tcaatatttc
      601 gtgcgagcgg gtacggccaa tgccgtagat gtaggtcagc gcgatctcca tccgcttctc
      661 gcgggggagg tctacgccgg cgagtcgtgc catgcgacgg tgtctcctgt ctcgtcgtac
      721 ccaggtctgc tccccgtccg tcccgtgctc gactcgtcgt tcacgggccc cagcctggcg
      781 tccgggggtg aaaccgatcc cgtctggatc ggctggatcg gggaggtctt ctcgcgccgt
      841 agaacggcga catcgtgcac tgctagccct gacgctgctt gtggcgctgg ttctcgcaga
      901 tcaccatgac cacgccgtgg cggcggatca ccttgcactt gtcgcagatc tttttgacgc
      961 tcggctgcac cttcacgtca tctctcctgg tgcgctactt gtagcggtag acaatgcggc
     1021 cacgggacag gtcgtagggc gagagctcca cgaccacccg gtcctcgggc aggatgcgga
     1081 tgtagtgctg gcgcatcttg ccgctgatgt gcgcgagaac gcggtgaccg ttctccaact
     1141 cgactcggaa catcgcgttg gggagtggtt cgaccacctt gccctctacc tcgatagcgc
     1201 cgtccttctt gcccatatcc tccgcgttcc tgctggttag ccgtgccgtc aggtggtggt
     1261 gttgccgcaa cacaggcacc ggccagttcc aagacgtggg gaggcaccag tacggagtgc
     1321 gggaacgcac cgctgagcag gaaacctgcg cagatacctg cagacacgct agaaaccggc
     1381 gcgatgctcg cgccgataac acagtttacg tccaggtcac gctcgggtcc aaaccgcccc
     1441 cgcgctgatc accgcgcggg cctgagggct cgcgcccgct cgcctatcca gccggccagc
     1501 agcaccgcac cccagggacc cgcaacccag acccaccacg gatagatcca gctcatggtg
     1561 gccagtgaca ccacggccca gatcagcagg ttcaccacgc tcacgctggc ccaggcggcg
     1621 accacagccc gcccgccgcg cccatgtcga cactgccagt gggattgctg cacctcgtgg
     1681 ttggctgggt cgaccggtgg acgagccgac ggcaggtcgg cggtcagcgc atcgagctcg
     1741 ccgtaggtcc gggctgccca ggcctgctgg agccgctcat cgtactcggt gaggtctagg
     1801 cgaccgtccg aatgcgccga tcttagctgc tcagccaccc gtttccgatc agtgtccgca
     1861 gcccgcatcc ggtctcgtgg cgtcgagcca tccacgatcc ccatcgtgcc ggtcatcgca
     1921 tccatcgata cctccagccg accctcggta ccaagcataa cactgttaca attcgcggtc
     1981 gtgcgattac cggcttggtc tccagaatcc agggctaggt ggtggccggc tcgggttccg
     2041 cgatagggct cgcgccgtgg atgggcccct cggcatcggc aagccgggtc tgggtggctc
     2101 tgccgcccca gcgcagggag atcaactcgg cagcgatgga taccgcggtt tcctcggggg
     2161 tgcgggctcc caggtccagc ccgatcggtg aggcgatctt ggacagctcc tcatcggtca
     2221 cgcctgcctc gcgcagccgc tcctcccggt ccttgtgggt cctgcgcgac cccatcgcgc
     2281 cgatgtaggc gaggtccagc ttcagcgcct cgacgagcac cggcacatcg aattttggat
     2341 cgtgagtaag gaccgtgatc acggtccgcg gatcgatccg gccagcctcg acctcctcct
     2401 tgaggtatcg gtgcggccag ttcacgatga cctcgtgcgc gtcggggaag cggctcttgg
     2461 tggcgaacac cggtcgcgca tcgcacaccg tcacgtagta gccgaggaat accccgatcc
     2521 gggccatcgc cgcagcgaag tcgatcgcgc cgaacaccag catccggggc ggcggctgca
     2581 tagcgttgac gaacacgtcc atgccctcgc ctcggcgctc cccttcgggg ccgtagtgca
     2641 gggtggccgt gcggccggcg gccagtgcgc cgcggacgtc ttcgaggatc gcctcgtcag
     2701 cgctggatga gcccgtcgtg ccggagtgcc gatcggccca gaccaccatg cgcgacccga
     2761 cgcgctcggg gtcgggatgg gcgaccacgg tggccaccgc gaccccctcc ttcttggcca
     2821 ccgaatcggc cacctcttcg agctcgggga agtggaccgg atccaccttc tcgacgaaca
     2881 catcgatgat gccgccgcag gtcagcccga cggtgaaggc atcatcatcg gagacgccgt
     2941 agcgctgcag gatgggggcg tcgtcggtga gcacctgctg gcccagttcg aagaccgcgc
     3001 cctccacaca gccaccagag acactgccga ccgcacgctc gtcggacgtc accagcatcg
     3061 acgctccagc cgggcggggc gccgagcgat aggtggatac caccgtgccc accgcggtgg
     3121 atttgccctc gcgccattcc gccaccagtt cgtcgagcac ctcacgcacc gcggacctcc
     3181 tcgagcaggg cttcccatgt agccaggcgg tgtcaggcca agtact
//
LOCUS       DATADN010000693         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_689335, whole genome shotgun sequence.
ACCESSION   DATADN010000693 DATADN010000000
VERSION     DATADN010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689335"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1569)
                     /locus_tag="VHH53_08965"
     CDS             complement(<1..>1569)
                     /locus_tag="VHH53_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX2290307.1"
                     /translation="REAGRRRAPILSESEFLTRREAVERTMIVRENLERTEIGVLEDD
                     ILVEHFVTASGSGSLVGNVYLGRVQNVLPSMEAAFVDIGRGRNAVLYAGEVDWDAAGL
                     EGKARRIEQALSSGDSVLVQVTKDPVGHKGARLTTQISLPGRFLVYVPGGRATGISRK
                     LPDTERKRLKEVLKRIVPEDAGVIIRTASEGVSEEELARDVRRLQAQWEVIQNRSGVS
                     ENGSGSSGSKAKAPELLYEEPDLLVKVVRDLFTEDFRELVVQGDDAWQTVEGYVKHVA
                     PDLMTRVRRHTGTGDVFADFRIDEQITKALDRKVWLPSGGYLIIDRTEAMTVIDVNTG
                     KFTGSGGNLEETVTRNNLEAAEEIIRQLRLRDIGGIIVIDFIDMVLESNRDLVLRRLT
                     ECLGRDRTRHQVAEVTTLGLVQMTRKKVGTGLLEAYSSTCEACKGRGVLVSAEPVNVR
                     SQPAAAPSGAKDSGGGNGRRGRSRGRGAGGDAVTSEQGTLEQGTLEQPGTDATPAGEM
                     APLPVPADSSEPVS"
BASE COUNT          267 a          559 c          463 g          280 t
ORIGIN      
        1 ccgaaacagg ttctgacgaa tccgccggca ccggcagcgg cgccatctca ccagcgggcg
       61 tggcgtcggt acccggctgt tccaaggtcc cctgttccaa ggtcccctgc tccgaggtca
      121 cggcatcacc acccgcgcca cggccacgag accgcccgcg gcggccgttg ccaccaccgg
      181 agtctttggc acccgagggg gccgcagccg gctggcttcg cacgttcacc ggctccgcgg
      241 ataccagcac gccgcggccc ttgcaggcct cgcacgtgct ggaatacgcc tccagcaatc
      301 cggtgccgac cttctttctg gtcatctgga ccagacccag cgtggtgacc tcggccacct
      361 ggtggcgggt gcgatcccgg cccaggcatt cggtcagccg acgtagcacg agatcccggt
      421 tcgactcgag caccatgtcg atgaagtcga tcacgatgat gccgccgatg tcgcgcagcc
      481 tgagctgacg gatgatctcc tcggcggctt cgaggttgtt acgggtaacg gtttcctcga
      541 gattgccgcc ggagccggtg aacttgccgg tgttgacatc gatcaccgtc atcgcctcag
      601 ttcggtcgat gatcaggtaa ccgccggaag gtagccacac cttgcgatcc agcgccttgg
      661 tgatctgctc atcgatccgg aaatcagcga acacgtcgcc ggtgccggta tgccggcgca
      721 cccgggtcat caagtccggg gccacatgtt tgacatagcc ctcaacggtc tgccaggcgt
      781 cgtcgccctg gaccaccagt tcgcggaagt cctcggtaaa caggtcccgg accaccttca
      841 ccaacaagtc tggctcctcg tacagcaatt ccggtgcttt tgccttgctc ccggagctcc
      901 ctgaaccgtt ttcggagaca ccagaacggt tttggatgac ctcccactga gcctgcaacc
      961 ggcggacgtc gcgagccaat tcctcctcgc tgacaccttc cgaagcggtc cggatgatca
     1021 cacccgcgtc ctcgggcacg atccgcttga gcacctcctt gaggcgcttg cgctcggtat
     1081 ccggcagctt gcggctgatg ccggtagccc gaccaccagg cacgtacacc aggaaacggc
     1141 ccggcaagct gatctgggtg gtcagccggg cacccttatg ccccaccggg tccttcgtca
     1201 cctgcacgag aacactgtcg ccgctggaca gggcctgctc aatgcgacgc gccttgccct
     1261 cgagcccggc agcgtcccag tccacctcgc cggcgtagag caccgcgttg cggccccgtc
     1321 cgatgtccac gaacgcggcc tccatcgagg gcagcacgtt ctgcaccctg ccgagataga
     1381 cgttgccgac gagtgatcca gagccggacg cggtgacgaa gtgctccacg aggatgtcat
     1441 cctcaagcac tccgatctcg gtgcgctcga ggttctcccg cacgatcatg gtgcgctcga
     1501 cagcctcacg ccgggtgagg aactccgatt cggacaggat cggagcccgc cgcctaccgg
     1561 cttcccggc
//
LOCUS       DATADN010000694         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_689401, whole genome shotgun sequence.
ACCESSION   DATADN010000694 DATADN010000000
VERSION     DATADN010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689401"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1912)
                     /locus_tag="VHH53_08970"
     CDS             complement(<1..>1912)
                     /locus_tag="VHH53_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2290308.1"
                     /translation="TYSPARVRYPYERGRLLELYREAKQRLKDPVLAWADVADDPEKA
                     RRYKSARGMGGFVRAEWWEAVEIVAAAHVHTIKRYGPDRVAGFSPIPAMSMGSHAVGA
                     RFISLVGGAMLSFYDWYADLPVASPQVFGDQTDVPESADWFDAGYLIMWGSNVPVTRT
                     PDAHYMTEARYRGQKVIVVSPDYGDHTKFADEWLPARPGTDAALAMAMGHVILKEFFV
                     DRQTPRFTDYVKRYTDLPFLVTLERRGEAYVPGKFLTAADLGDTSEGAKSKTVLLDGV
                     TGQPVVPNGSLGHRFTASGEGHWNLDLHGVDPLLSMHGDGEVEVHLPRFDTAEPGVLR
                     RGVPTRMVAGRLVTTVFDLLLAQYGVHRPGLPGTWPTGYDDAEQPCTPAWQKPITGVP
                     AAAAERIGREFADNAARSGGRSMILMGAGTNHWFHSDQTYRSFLALVMLTGCQGVNGG
                     GWAHYVGQEKCRPITGWSTLAFGLDWQRPPRQMQGTVYWYLATDQWRYDPFTAEAMAS
                     PLAKGRFAGRTAADNIALASRLGWMPSYPTFNRNPLDLADEAQRLGKNAAQHVVDGLS
                     TGHLRFACEDPDAPENFPRCLTVWRANLLGSSAKGNEYFLRHLLGSDSNLQATDNDGV
                     RPVEVTWRDEAPV"
BASE COUNT          294 a          655 c          658 g          305 t
ORIGIN      
        1 acgggcgctt cgtcgcgcca ggtgacctcg acgggccgca ccccgtcgtt gtcggtggcc
       61 tgcaggttgg agtcgctgcc caacaggtgg cgcaggaagt attcgttgcc cttcgccgac
      121 gaaccgagca ggttggcccg ccacacggtc aggcaccgcg ggaagttctc cggcgcgtcc
      181 ggatcctcgc aggcgaacct caggtgcccg gtggacagcc cgtcgacgac gtgctgcgcg
      241 gcgttcttgc ccaggcgttg cgcctcgtca gccaggtcca gcgggttgcg gttgaacgtg
      301 gggtaactgg gcatccagcc cagccggctg gccagcgcga tgttgtcggc cgcggtccgg
      361 ccggcgaaac gccctttcgc cagcggtgag gccatcgcct cggcggtgaa cgggtcatag
      421 cgccactggt cggtggccag gtaccagtac acggtgccct gcatctgccg cggcggccgc
      481 tgccagtcca ggccgaaggc cagcgtcgac cagccggtga tcggccggca cttctcctgg
      541 ccgacgtagt gcgcccagcc gccgccgttg accccctggc acccggtgag catcaccagt
      601 gccaggaacg accggtaggt ctggtcggaa tggaaccagt gattggtgcc cgcgcccatc
      661 aggatcatcg atcggccgcc cgagcgcgcg gcgttgtcgg cgaactcccg gccgatccgc
      721 tccgctgcgg ccgccggtac gccggtgatc ggcttctgcc aagccggtgt gcacggctgc
      781 tcggcgtcgt cgtacccggt gggccaggtc cccggaaggc ccgggcggtg caccccgtac
      841 tgcgccagca gcaggtcgaa caccgtggtg accagcctgc cggcgaccat cctggtcggc
      901 accccccggc gtagtacgcc gggctcggcg gtgtcgaacc gggggaggtg gacctcgacc
      961 tcgccgtcac cgtgcatgct caggagcgga tcgacaccgt gcaggtcaag gttccagtga
     1021 ccttccccag aggcggtgaa ccggtggccc agcgaaccgt tcgggaccac cggttgcccg
     1081 gtgaccccgt ccagcaggac ggtcttggac ttggcgccct cgctggtgtc acccagatcg
     1141 gccgcggtga ggaacttgcc ggggacgtac gcctcgccac gccgctcgag agtgaccagg
     1201 aacggcagat cggtgtagcg cttgacgtag tcggtgaagc ggggcgtctg ccggtcgacg
     1261 aagaattcct tgaggatcac gtgacccatg gccatggcca gcgcggcgtc ggtgcccggc
     1321 cgggcgggca accactcgtc ggcgaatttt gtgtgatcac cgtagtccgg cgacacgacg
     1381 atcaccttct ggccccggta gcgggcctcg gtcatgtaat gcgcatcagg ggtgcgggtg
     1441 accgggacgt tggagcccca catgatcagg taaccggcat cgaaccagtc ggcggactcg
     1501 ggcacgtcgg tctggtcacc gaatacctgc ggggacgcta ccggcagatc ggcataccag
     1561 tcgtagaacg acagcatggc cccgccgacc agcgagatga accgggcgcc gacggcgtgg
     1621 ctgcccatcg acatcgccgg gatcggtgaa aatccggcga cccggtcggg accgtagcgc
     1681 ttgatcgtgt gcacatgcgc cgccgcgacg atctcgacgg cttcccacca ctcggcgcgc
     1741 acgaacccgc ccatgccacg cgcagacttg taccggcggg ctttctcggg atcgtcagcc
     1801 acatcggccc atgccagcac cgggtccttg agtcgttgct ttgcctcccg gtacagctcc
     1861 agcagcctgc cgcgctcata cgggtagcgc accctggctg gcgagtaggt gt
//
LOCUS       DATADN010000695         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_542533, whole genome shotgun sequence.
ACCESSION   DATADN010000695 DATADN010000000
VERSION     DATADN010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542533"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VHH53_08975"
     CDS             <1..1256
                     /locus_tag="VHH53_08975"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEX2290309.1"
                     /translation="GGYYYWFGENRNPDNTFRAVSVYRSTDLRTWEFRNNVLTQSSHS
                     ELQVANIERPKVIFNPSTGQYVMWMHKENGRDYGEARAAVATASTVDGNYTWRGSFRP
                     LDFMSRDITLFRDDDGTAYMISAANENADLHVYRLTSDYLQVSSRVQRLWPGSWREAP
                     ALFKRNGVYFLLTSGATGWAPNQQKYATASSVTGTWSSLSNVGDATAFDSQTAFVLPV
                     QGSQTTSYLYLGDRWAGATGGPVNESRYVWLPLNFPSNTTMTMSYSSQLTIDTATGTI
                     TPAGVQRYSFTARHSGKCADIPSSSQSNGTQLKQYPCNGGNNQRFDAQPAGGEYYRLV
                     AVHSGKCLDVSDVSPADGATVIQYTCGSGTNQQWRFEDAGNGYYRIVARHSGKCLDVT
                     SASTADGAVIKQYTCGSGTNQQWRR"
     gene            1266..>2264
                     /locus_tag="VHH53_08980"
     CDS             1266..>2264
                     /locus_tag="VHH53_08980"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEX2290310.1"
                     /translation="MQALSRRWLARLSIVALTAAATAVVVGSPAQAAPGYVYTTFKGD
                     AAADQELWVYSSANGTNFSVLADTNYRGPSGVLRDPSVIQHNGLYYIAHTVQSWTTNS
                     TYFNIASSPDLVTWTHVASVNSGVANTRFTWAPEFYVEGGTIRIIVSIAQTTCSNCFR
                     PYIFTAQNGSLTSWSGPVQMGGLGYNYIDTYVVKSGSTYHAFAKNETSKYIEHWTSTS
                     LTSGWSNQGQLWSSGYEGPAVLRMSDGSWRIWIDRYTNGGIWTATSSNLNSWSGLSQI
                     TCPGCRHGTILPVADVPGSAPVYRINVRHSGKAMDVQQPNTDNGARVGQYTYGGNPW"
BASE COUNT          439 a          793 c          685 g          347 t
ORIGIN      
        1 tcggcggcta ctactactgg ttcggggaga accggaaccc cgacaacacg ttccgggcgg
       61 tttcggttta ccggtcgacc gacctgcgca cctgggagtt ccgcaacaac gtgctgaccc
      121 agtcgtcgca ctcggaactc caggtggcga acatcgagcg acccaaggtc atcttcaacc
      181 cgtcgaccgg ccagtacgtg atgtggatgc acaaggagaa cggccgggac tacggcgagg
      241 cacgcgccgc ggtcgccacc gccagcacgg tcgacggcaa ctacacctgg cgcggcagtt
      301 tccgcccgct ggacttcatg tcccgcgaca tcacgctgtt ccgcgacgac gacggtaccg
      361 cctacatgat ctccgcggcg aacgagaacg ccgacctgca cgtctaccgg ctgacctcgg
      421 actatcttca ggtcagctcc cgggtgcagc ggctgtggcc gggttcgtgg cgtgaggcgc
      481 cggcgctgtt caaacgcaac ggcgtctact tcctgctcac ctccggcgcc accggctggg
      541 cacccaacca gcagaagtac gccaccgcaa gcagcgtgac cggaacctgg agctcgctgt
      601 cgaacgtcgg cgacgcaacc gcgttcgact cgcagaccgc gttcgtgctc cccgtccagg
      661 gcagccagac cacgtcgtac ctgtacctgg gtgaccgctg ggccggcgcg accggcggac
      721 cggtcaacga atcccgctat gtctggctac cgctgaactt cccgtccaac accacgatga
      781 cgatgagcta ctcgtcgcag ctgacgatcg acaccgccac cggcacgatc accccggccg
      841 gcgtgcagcg gtattcgttc accgcgaggc acagcggaaa atgcgctgac attcccagtt
      901 ccagccagtc caacggaacc cagctgaaac aatatccgtg caacggcggc aacaaccaga
      961 gattcgatgc ccagccggcc ggcggtgaat actatcggct ggtcgccgtg cacagcggca
     1021 agtgcctcga cgtctccgac gtgtcgcccg cggacggcgc caccgtgatc cagtacacct
     1081 gtggcagcgg taccaatcag caatggcggt tcgaggacgc gggcaacggt tactaccgga
     1141 tcgtcgcacg gcacagcgga aaatgcctgg acgtcaccag cgcgtcgacc gccgacggtg
     1201 cggtcatcaa gcagtacacc tgtggttccg gcaccaacca gcagtggcgc aggtgaggag
     1261 gcgtgatgca ggcactgtcc aggcgctggc tggcccgact cagcatcgtg gcgctcactg
     1321 ccgccgccac tgcggtggtc gtcggatcac ccgcacaagc cgcccccggc tacgtctaca
     1381 ccacgttcaa gggcgacgcc gctgccgacc aggagctctg ggtctactca tcggccaacg
     1441 gcacgaactt cagcgtgctg gccgacacca actaccgcgg tccgtccggc gtgctgcgcg
     1501 accccagcgt catccagcac aacgggctct actacatcgc ccacaccgtc cagtcgtgga
     1561 ccacgaactc gacgtacttc aacatcgcgt cgagtcctga cctggtcacc tggacgcacg
     1621 tggccagcgt caactccggc gtcgccaaca cgcggttcac gtgggcgccc gagttctacg
     1681 tcgagggcgg caccatccgg atcatcgtga gcatcgccca gacgacgtgc tcgaactgtt
     1741 ttcggcctta catcttcacc gcccagaacg gttcgctgac gtcctggagc gggccggtgc
     1801 agatgggtgg cctcggctac aactacatcg acacctacgt ggtgaagtcg ggtagcacct
     1861 accacgcctt cgccaagaac gaaacctcca agtacatcga gcactggacc agcacgagcc
     1921 tcaccagcgg gtggagcaat cagggccagc tgtggagctc gggctacgag gggccggccg
     1981 tgttgcggat gagcgacggc tcctggcgca tctggatcga caggtacacc aacggcggca
     2041 tctggacggc gaccagctcg aacctgaact cctggtcggg cctgagccag atcacgtgcc
     2101 ccggttgccg ccacggcacg atcctgccgg tagccgacgt gcccggctcg gcgccggtct
     2161 accggatcaa cgtgcgccat agcggcaagg ccatggacgt ccaacaaccg aacaccgaca
     2221 acggtgcgcg cgtcggccag tacacgtacg gcggcaaccc gtgg
//
LOCUS       DATADN010000696         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_998918, whole genome shotgun sequence.
ACCESSION   DATADN010000696 DATADN010000000
VERSION     DATADN010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998918"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..>1732)
                     /locus_tag="VHH53_08985"
     CDS             complement(150..>1732)
                     /locus_tag="VHH53_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEX2290311.1"
                     /translation="NGAPVRRSAGAEAARLLADLVIESARTLVFVRSRRGAELTALGA
                     QRALSAAAADDLTTRIAAYRAGYLPEERRALERALDSGELLGVASTNALELGVDIAGL
                     DAVVLAGYPGTRASFWQQAGRAGRAGSGALVVFVARDDPLDTYLVHHSAALLGSAVEA
                     TVLDPLNPYVLRPQLACAAAELPLTEACVDELGGEVARSVVAGLVADGSLRRRSGGWY
                     WAAARQRPHCEVDIRGCGGHQVAVVEADTGRLLGTVDPGSACTAVHPGAVYLHRGDSF
                     VVDELDLDVGVALVHPEEPEWHTSARSTTDICVVSVLEQRCSGGVSVTLGEVEVTSQV
                     VGYLRRLPSGQVLDAVPLELPATTLRTRAVWYTVSEQLLVGAGLPQASWPGALHAAEH
                     AAIGLLPLVATCDRWDIGGVSTALHPDTGAPTVFVHDGHPGGAGFADRGHAALCPWLT
                     ATRDAIAACECPAGCPSCVQSPKCGNGNDPLDKAGAIKVLDLVLTSLPAVAPDPAQRA
                     PRQSLPPAAAAPSRTSRA"
BASE COUNT          288 a          629 c          584 g          231 t
ORIGIN      
        1 cacgccgacc gggcggtggc tgttgccggt actcggtggc gcgctgcatc gggagttgga
       61 tgaccatccc gacgttgtgc tgcatgccca gggcgactgg gtgccgttgc cgccgtcgcc
      121 gtgcttgcct ggtgtcgtgc attggcgagt caagcccgcg atgtgcggct gggagctgcc
      181 gcggctggcg gaagtgactg acgcggtgct cgctgcgctg gatcaggtgc aaccgctggc
      241 agcgaggtga gcacgaggtc gagcaccttg atcgcaccgg ccttgtccaa cggatcgttg
      301 ccgttaccgc atttgggaga ctgaacacaa gaggggcaac cggctgggca ctcacacgcg
      361 gcgatggcgt ctctggtcgc agtcagccac gggcagagcg cagcatggcc tcggtcggcg
      421 aacccggctc caccgggatg cccgtcatgc acgaacaccg ttggcgctcc ggtgtccgga
      481 tgcagcgccg tggagacgcc accgatatcc caccgatcgc aggtggcgac caggggcagc
      541 agaccgatcg cggcatgctc ggcggcgtgc agtgccccag gccacgaggc ctggggcaac
      601 ccggcgccga cgagcaactg ctccgacacc gtgtaccaga ccgcccttgt ccgcagggtg
      661 gtcgccggta gctccaacgg gacagcatcg agcacctggc cagacggcag ccgccgcagg
      721 taaccaacga cctgtgaggt cacttccacc tcgcccaacg tcaccgacac cccacccgag
      781 caccgctgct ccagcaccga caccacgcag atatcagttg tcgagcgggc cgaggtgtgc
      841 cactccggct cctcgggatg gaccagcgcg acgccgacgt caagatcgag ctcgtcgacc
      901 acgaaggagt caccccggtg cagataaacc gcgcccgggt gcaccgccgt gcacgccgat
      961 ccggggtcta ccgtgccgag cagacgaccg gtatcggcct ccaccaccgc cacctgatgc
     1021 ccaccgcagc cgcggatgtc gacctcacaa tgtgggcgct gtcgggccgc ggcccaatac
     1081 cagccgccgg aacggcggcg caacgatccg tcggccacca ggccggcgac cacggaccgc
     1141 gccacctcac cccccagctc gtcgacgcag gcttcggtga gcggtagctc ggcggccgcg
     1201 caggctagct gcggtcgcag cacataggga ttcagcggat ccagcacggt ggcctcgacc
     1261 gccgagccca acaaagccgc actgtggtgc accaggtagg tgtcgagcgg gtcgtcccgg
     1321 gcgacgaaca ccacgagcgc tccggatccg gctcgtccgg ctcgcccggc ctgctgccag
     1381 aaagacgctc gggtgccggg atagccggcc aacaccaccg catcgagccc ggcgatgtcc
     1441 acgccgagct ccagcgcatt tgtcgaggcc acgcccagca gctccccgga atccagcgct
     1501 cgctccagcg ctcggcgctc ctcggggagg taacccgccc ggtaggcggc gatgcgggtg
     1561 gtgaggtcat ccgccgcggc agccgacagc gcccgctggg cccccagtgc ggtcagctcg
     1621 gcgccccggc gggagcggac gaacaccagg gtccgggcgc tctcgatcac caggtcggcc
     1681 agcaaccggg cagcctcagc tcctgccgag cggcgcaccg gtgcgccgtt ct
//
LOCUS       DATADN010000697         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1280459, whole genome shotgun sequence.
ACCESSION   DATADN010000697 DATADN010000000
VERSION     DATADN010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280459"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VHH53_08990"
     CDS             <1..196
                     /locus_tag="VHH53_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290312.1"
                     /translation="RVIALAVAAVGIAAAILAAHAFRELPWNQLSALVLAALALCAVA
                     LLAAYWTIAAALWFRHHSGE"
     gene            complement(350..1069)
                     /locus_tag="VHH53_08995"
     CDS             complement(350..1069)
                     /locus_tag="VHH53_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2290313.1"
                     /translation="MTSYVLIPGAGGVAWYWHLLVAELQVRGHDVVAVGLPAADDSAG
                     LTEYADTVVDAIGDRTDVVVVAQSLAGFTAPLVCERVPVTLLVLLNAMIPAPGESPGD
                     WWANTGHSQARAEQAARDGRMLAGDEDLLDAFFHDVPPDVTAEAMARGEPVQSATPFV
                     QPWPLRRWPRVPTRFLQGRDDRFFPVEFQRRVAQDRLGITPDEMLGGHLVALSRPKDL
                     ADRLEAYWADHKPASPPSLVH"
     gene            complement(1471..>1863)
                     /locus_tag="VHH53_09000"
     CDS             complement(1471..>1863)
                     /locus_tag="VHH53_09000"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEX2290314.1"
                     /translation="DRTFVSGMQQINVAILNRWFFTTFLGAPLVTALAGLLQLGAGSQ
                     AVLLWIVAAFILSAVAFVITIVVNVPLNDALAAAGDPTQVADLAAVRAQFEPSWVRWN
                     VARAIAFTAAFGSLTWALVLYGCATATI"
BASE COUNT          323 a          628 c          624 g          288 t
ORIGIN      
        1 gcgggtgatc gcgctggccg ttgcagcggt gggcatcgcg gcggcgatct tggcagcgca
       61 cgcattccgg gaactgccct ggaaccagct gtccgcgctg gtgctcgccg cgctcgccct
      121 ctgcgccgtc gcgctactcg ccgcgtactg gacgatcgcc gctgcgctct ggttccggca
      181 ccactcaggg gaatgatcat cagctgcgac atgtcatgtg cggcgacaca ccgatcgcga
      241 gttattgcgg aatctgcggt caacgctaga cagccgcata cggcgacgct atcgctcgcg
      301 ccgggccacg atcgtcagca actcgccgta ccgagcccgt agtcgccggt cagtgcacca
      361 gggacggcgg gctggccggc ttgtggtccg cccagtaggc ctcgagccgg tccgccaggt
      421 ccttggggcg gctgagggcg acgagatgcc cgccgagcat ctcgtcgggt gtgatgccca
      481 ggcggtcttg ggcgacccgg cgttggaact ccaccgggaa gaagcggtcg tcgcggccct
      541 ggagaaatct tgtaggaacc ctgggccacc ggcggagtgg ccacggctgc acgaacggtg
      601 ttgcggactg gactggctct ccccgcgcca tcgcctctgc tgtcacatcg ggtgggacgt
      661 cgtggaagaa ggcgtcgagc aggtcctcat cgccagcgag catcctgccg tcgcgagcag
      721 cttgctccgc tcgggcttgg gaatgcccag tgttggccca ccagtcgcct ggcgactcgc
      781 ccggtgcggg aatcatggcg ttgagcagca cgagcagagt caccggcacc cgctcacaca
      841 ccagcggggc ggtgaacccc gccaacgact gggcgaccac gacgacgtcg gtgcggtctc
      901 cgatggcgtc aaccacggtg tcggcgtatt cggtcaatcc ggccgaatca tcagcagccg
      961 gcaggccgac ggccacgacg tcgtggccac gcacctgcag ctcggcgacc agcaggtgcc
     1021 agtaccaggc gacgcctcca gcaccgggaa tcaatacgta gctcgtcaca ggtacctcct
     1081 cgccagcgcc agtggccggt gcaacgcggc agccgagagg aaactctcgg ccgcagtcac
     1141 gatgtctatg actgctggcg ccccttggaa tcatcggggg gcagttgcag ccttatcaag
     1201 cacgtaagcg ccaccggccg cctgcgctgt aaccactact ccaacgccaa tggccgcacc
     1261 cagcgcagtt gccaccgctc cgggtcacgc gcggaggtgc ggttggcgcc gcggtggtca
     1321 ccccgatgcc acgcgtgact tcctggccaa cgcgcagcag caggacggct ggggctcgcg
     1381 tcattgctgc agaacatcga ccccacggcc agcaaagctc cggcatcccg tagagctgac
     1441 ttgacgcagc atgggaggcg atgaccgacc tcaaatcgtc gcggtggcgc atccgtacag
     1501 caccagtgcc caggtgaggc taccgaatgc agcagtgaac gcgatcgcgc gggcaacgtt
     1561 ccaacgcacc cagcttggct cgaactgtgc ccgcaccgcc gcgaggtcgg cgacctgcgt
     1621 ggggtcgccg gctgcggcga gcgcgtcgtt gagcggtaca ttcaccacga tagtgatcac
     1681 gaatgccacc gcggacagga tgaatgcggc cacgatccag agcagcaccg cctggctgcc
     1741 tgcgcccagc tgcaatagcc cggccagcgc ggtcaccagg ggtgcgccga ggaaggtggt
     1801 gaagaaccag cggttgagga tcgccacgtt gatctgttgc atgccgctga cgaaggtgcg
     1861 gtc
//
LOCUS       DATADN010000698         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_396556, whole genome shotgun sequence.
ACCESSION   DATADN010000698 DATADN010000000
VERSION     DATADN010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396556"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..>1851
                     /locus_tag="VHH53_09005"
     CDS             16..>1851
                     /locus_tag="VHH53_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2290315.1"
                     /translation="MRLRRTTGTAPKSSAPPLIGVDDLKTPPWAQVMETVAGTGISRT
                     LRAAPAALRVLVRLAWQTSPRLTLLAAAVELLAGCATAFGLLATANVLTELLAQGPTP
                     QRVVAALPALGLVMASFAARGLLDAAVGAVQAVLAPRVELRAQNELHAAVIGVELLAF
                     DDPDFVELVRRTSAQGIRSVRYGVGDVGNLVSSAVSLAAAIVTAGLLNPVLAPVVLLA
                     AVPNGWANVRAALLSYESYVRTVSRMRRLGITGDIITGRREAAEVRAFTTQDTLLGEH
                     RRIGSQLTAEAVSVEHRKTAIRLFGRGLAGIGTALAYGVLGLLLYAGAMPLALAGAAA
                     VAMRTASTAVSNTVFAANRLFEHSFYLDMLTCCLTQARSQHRSAAGLRLPRDPQLIEL
                     HNVSFSYPGKDESALHQVNVTIHRGQVVALVGENGSGKSTLAKIITGLYLPDLGRVTW
                     DGVDIAKVDQRELHSRVAVVLQDPVEWPMTAENNIRIGRLERPDPDGSVLAAAAADSG
                     ADAVVAELPDGWASVLSREFQNGCDLSGGQWQRISVARGLYRDAPLLIADEPTAAMDA
                     RAEHAAFAALRSMSTTGDDGRGRITVLVTHRLANVRHADQIVVLDH"
BASE COUNT          261 a          634 c          663 g          293 t
ORIGIN      
        1 cggtgggagg ccacgatgag actcaggcgg accacgggca ccgccccaaa gtcatcagca
       61 ccaccgctga tcggggttga tgatctcaag accccgccct gggcgcaggt gatggagaca
      121 gtggccggca cgggtatcag ccggacgttg cgggcggcgc cggcggcact gcgcgtgttg
      181 gtgcgcctgg cgtggcagac gtcgccgcgg ttgacgttgc tcgccgcggc tgtcgagctg
      241 ctcgccggat gcgcgacggc gttcgggctg ctggccaccg cgaacgtgct gaccgagctg
      301 ctggcgcagg ggccgactcc gcagcgggtg gtggcggcgt tacccgcgct ggggttggtg
      361 atggcctcct tcgccgccag gggcctgctg gacgcggcgg tgggagccgt gcaggcggtg
      421 ctggcccccc gggtcgagct gcgcgcccag aatgagctgc acgccgcggt gatcggcgtg
      481 gagctgcttg ccttcgacga tccggatttc gtggaactgg tgcgccggac gtcggcgcag
      541 ggcatcagga gcgtgcgcta cggggtgggc gatgtcggaa acctggtcag ctcggcggtc
      601 tcgctggccg ccgccatcgt cacggcgggc ctgctcaacc ccgtactggc gccggtggtg
      661 ctgctggccg ccgtgcccaa tggctgggcc aacgtccgcg cagcgctgct gtcctacgag
      721 tcgtatgtgc gtacggtctc gcgcatgcgc cggctgggca ttaccggcga catcatcacc
      781 ggtcggcgcg aggcagccga ggtccgcgcc ttcaccaccc aggacaccct actgggcgag
      841 caccgccgga ttggatccca gctcaccgcg gaagccgtca gcgtggagca ccgcaagacc
      901 gcgatccggc tgttcgggcg gggtctggcc gggatcggca cggcgttggc ctacggcgtg
      961 ctcggtctgc tgctctacgc cggggcgatg ccgctggcgt tggccggggc ggcagcagtc
     1021 gcgatgcgca cggctagcac ggcggtgtcc aacacggtat tcgccgccaa ccggctcttc
     1081 gagcacagtt tctaccttga catgctgacc tgctgcctga cccaggcacg cagccagcac
     1141 cggtccgctg ccgggctgcg gctgccccgc gatccccaac tcatcgagtt gcacaacgtg
     1201 tccttcagct accccggcaa agacgaatct gccctgcatc aggtcaacgt gaccattcac
     1261 cgcgggcagg tcgtggctct ggtgggggag aacggctcgg gcaaatccac tctcgccaag
     1321 atcatcactg ggctttacct gcctgaccta ggacgggtga cgtgggacgg ggtcgacatc
     1381 gccaaggtcg accagcgtga gttgcactcc cgggtcgccg tcgtcctgca ggaccccgtc
     1441 gagtggccaa tgaccgccga gaacaacatc cgcatcggcc ggttggagcg tcctgacccc
     1501 gacgggagcg tgctcgccgc cgcggccgcc gactccggcg ccgatgccgt cgtcgccgag
     1561 ctacccgatg gctgggccag tgtgctgtct cgggagttcc agaacgggtg cgacttatcg
     1621 ggtgggcagt ggcagcggat cagcgtggcc cgtggcctgt atcgggacgc ccccctgctc
     1681 atcgccgacg agcccaccgc cgcgatggac gcccgcgccg agcacgccgc gttcgccgcg
     1741 ctgcggtcga tgtccaccac gggggacgac ggccgcgggc ggatcaccgt gctcgtcacg
     1801 caccggctgg cgaacgtccg gcacgccgac cagatcgtcg tgctcgacca c
//
LOCUS       DATADN010000699         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_829489, whole genome shotgun sequence.
ACCESSION   DATADN010000699 DATADN010000000
VERSION     DATADN010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829489"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..425
                     /gene="rpsF"
                     /locus_tag="VHH53_09010"
     CDS             126..425
                     /gene="rpsF"
                     /locus_tag="VHH53_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX2290316.1"
                     /translation="MRHYEMMVILDPQLDERTVAPSLEQFLGVVRTQGGSVDKVEVWG
                     KRRLSFEINKRTEGIYAVVNISCTPDTVAELDRQLSLSETILRTKVLRRETGKKG"
     gene            427..933
                     /locus_tag="VHH53_09015"
     CDS             427..933
                     /locus_tag="VHH53_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2290317.1"
                     /translation="MAGETVITVIGNLTADPELRFTPSGAAVANFTVASTPRTFDRQT
                     GEWKDGEALFLRCNIWRQAAENTAESLTRGMRVIVSGRLRQRSFETREGEKRTVVEME
                     VDEVGPSLRYATAKVNKVSRQGGPSGSGGYGSSGSPADDPWSSAPQVGAPAGSGGGAA
                     GFDDEPPF"
     gene            973..1209
                     /gene="rpsR"
                     /locus_tag="VHH53_09020"
     CDS             973..1209
                     /gene="rpsR"
                     /locus_tag="VHH53_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX2290318.1"
                     /translation="MAKPPIRKPKKKICIFCKDSDAVIDYKDTVLLRKYISDRGKIRA
                     RRVTGNCSQHQRDVAVAVKNAREVALLPYTSTAR"
     gene            1230..1685
                     /gene="rplI"
                     /locus_tag="VHH53_09025"
     CDS             1230..1685
                     /gene="rplI"
                     /locus_tag="VHH53_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX2290319.1"
                     /translation="MKIILTADVPNLGTPGDLVQVKDGYARNYLLPRNLAIVATSGAE
                     KQVATIRRARQARQIRDLDHAREVAGNLSGLGTVSVTAKAARSGGKLFGSVTVADIVD
                     AVRSAGGPALDKRAVDLGGSIKSTGDYQVTVRLHPEVSVTVPVKVTTAG"
     gene            complement(1669..>1948)
                     /locus_tag="VHH53_09030"
     CDS             complement(1669..>1948)
                     /locus_tag="VHH53_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290320.1"
                     /translation="PGVPGRRSTKVRMVNAYLPRLHAAASTDSSLGRAFMRVVGMLDR
                     PEGLVRPDRALRVLWAHLRGTPAPADGPVLVGNAGGPASCPPDLSQRW"
BASE COUNT          344 a          639 c          646 g          319 t
ORIGIN      
        1 tcgcgagacg gcacaggttt atcgccagcg cagctggcgg cagttcaatt gccggctcag
       61 ccggcggcat cctcctgccg cggagagtcc gcggccgacc agtccacagg aggtcagtgg
      121 tcttcatgcg tcattacgag atgatggtca tcctcgatcc gcagctcgat gagcgcaccg
      181 ttgcgccatc gctggagcag ttcctcggcg ttgtgcggac acagggcggc agcgttgaca
      241 aggtcgaggt ctggggcaag cgcaggctgt ccttcgagat caacaagcgc accgagggca
      301 tttacgcggt ggtcaatatc tcctgcactc ccgacaccgt ggccgagctc gaccggcagc
      361 tctcactgag cgagacgatc ctgcgcacca aggtgctgcg gcgcgagacc gggaagaagg
      421 gctgacgtgg ctggtgaaac tgtcatcacg gtgatcggca atctgaccgc cgatccggag
      481 ctgcggttca ccccgtccgg cgcggcggta gcgaacttca cagtggcgtc caccccacgt
      541 actttcgacc ggcagaccgg cgagtggaaa gacggcgaag cactcttcct gcgttgcaac
      601 atctggcggc aagccgcgga gaataccgcc gagtcgctca cccgtggcat gcgtgtgatc
      661 gtcagcgggc ggctgcgcca gcgctcgttc gaaactcgcg agggcgagaa gcgcaccgtc
      721 gtcgagatgg aggtcgatga agtcggcccc tcgctgcgct atgcgaccgc caaggtcaac
      781 aaggtgagcc gtcagggtgg cccgtcgggt agtggcggct atggctcgtc cgggtcgccg
      841 gccgacgatc cgtggagctc ggcaccccag gtcggtgcgc cggccggatc tggcggcggc
      901 gccgctggtt tcgacgacga gcccccgttc tgatcatccc ttctcggcga cccattcagg
      961 agtaccccca tcatggccaa gccaccgatt cgtaaaccga agaagaagat ctgcattttt
     1021 tgtaaggact ccgacgccgt tatcgactac aaggacacgg tgctgttgcg gaagtacatc
     1081 tccgaccgcg gcaagatccg tgcccgtcgg gtcaccggca actgcagcca gcaccagcgc
     1141 gatgtcgcgg tcgcggtgaa gaacgcccgc gaggtggccc tgctgcccta cacgtcgacc
     1201 gcacgctgag ccgaggaaga agcgatcacg tgaagatcat ccttaccgct gacgttccga
     1261 accttggcac gcccggcgat ctggtccagg tcaaggatgg atacgcccgc aactacctgc
     1321 ttccgcgcaa cctggccatt gtggccacca gcggcgcgga gaagcaggtg gcgaccatcc
     1381 gccgggccag gcaggcgcgg cagatccgcg atctcgatca tgcccgggaa gtcgctggta
     1441 acctgtcggg tctgggcaca gtgtccgtga cggccaaagc cgccaggtcc ggcgggaagc
     1501 tgttcggttc ggtcacggtg gccgacatcg tcgatgcggt ccgctcagcg ggcggccccg
     1561 cgttggacaa gcgagctgtc gacctcggtg gatcgatcaa gtccacaggt gattaccagg
     1621 tgaccgtccg gctgcacccg gaggtctcgg tgaccgtccc tgtcaaggtc accaccgctg
     1681 gctgaggtca ggtggacagc tggcgggccc gccggcgttg cccaccaaga ccggtccatc
     1741 cgctggggct ggtgtgcctc gcaggtgtgc ccacagcacg cgcaatgccc gatccggccg
     1801 caccagccct tcgggccggt cgagcattcc caccacgcgc atgaaggccc gccccagcga
     1861 gctatcggta ctggccgcgg catgcagccg gggcaggtag gcgttgacca tccgcacctt
     1921 ggtgctacgc cggcccggca cgccgggg
//
LOCUS       DATADN010000700         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_543146, whole genome shotgun sequence.
ACCESSION   DATADN010000700 DATADN010000000
VERSION     DATADN010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543146"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VHH53_09035"
     CDS             <1..221
                     /locus_tag="VHH53_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680419.1"
                     /note="Required in the synthesis of PPQ, but its exact
                     function is unknown; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein C"
                     /protein_id="HEX2290321.1"
                     /translation="ALRKYYPWIDQDALVYFDSRIGHARLECADALDIVLSHCTSREG
                     QDAALHALAFKTDVLWSILDAIEHALLR"
     gene            221..499
                     /gene="pqqD"
                     /locus_tag="VHH53_09040"
     CDS             221..499
                     /gene="pqqD"
                     /locus_tag="VHH53_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEX2290322.1"
                     /translation="MAALSLTDRPQLRRGVRAGTDPLSGETVLLFPEGVLMMNETAAA
                     VVRHCDGNHSVADVLQSIGEAYDDVAADDVMSLLHDLIAQGLLVAGDG"
     gene            492..1577
                     /gene="pqqE"
                     /locus_tag="VHH53_09045"
     CDS             492..1577
                     /gene="pqqE"
                     /locus_tag="VHH53_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEX2290323.1"
                     /translation="MDRPLGLLAELTYRCPLHCPYCSNPVAVPQAAELALQEWLGVLD
                     EARQLGVLQLHLTGGEPLARPDLTPLVARARDLGFYVNLVTSGVGLDERRATELAQAG
                     LDHVQLSIQDADRTAADEVAGARVWDRKIAAARVVTALALPLTINVVLHRGNISRIGP
                     LVELAAELGADRLELAHTQYYGWALLNRSALLPTREQVVAADHAVTQARAAYGDTLEI
                     LYVVADYHEPYPKPCMHGWGRRHIIITPDGRVLPCPAAGQITGLDIDNVREHSLRTIW
                     QHSAAFTRFRGSGWMAEPCRSCPRKEVDFGGCRCQAFQLTGDAARADPVCQLSADHDL
                     IRAVVDATDPQQPLRPRVNPDRQRPHQ"
     gene            1695..2084
                     /locus_tag="VHH53_09050"
     CDS             1695..2084
                     /locus_tag="VHH53_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290324.1"
                     /translation="MDGLYGIPETREHQYMLTHIEDDQRVADVGDDRGTRPVHDAAGP
                     ITQRAYDSMAQLVRQGLDTSLRSVQVWADLARQVDSTAVTSAYDPFEQLLATQREIVG
                     DLVATQRQLAQQVFDTSAAAGGAAVPR"
     gene            2375..2641
                     /locus_tag="VHH53_09055"
     CDS             2375..2641
                     /locus_tag="VHH53_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290325.1"
                     /translation="MSNSLQLAHQWWAALGQADRQRVWDSRHSYLAGDLVESMAKAGV
                     PVVSDGRWSCVSIGPTGFTVPTAVEQLLKGLSRTPDGDRPLGNA"
     gene            complement(2731..>3250)
                     /locus_tag="VHH53_09060"
     CDS             complement(2731..>3250)
                     /locus_tag="VHH53_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX2290326.1"
                     /translation="LAKQLTAAGLPAVELHGNLSQNARARNLAAFSEGSARVLVATDI
                     AARGIHVDEIGLVIHVDPPAEYKGYLHRSGRTARAGAEGIVVTLSTPDQSGEMRTLTR
                     QAGIRPVTTRVRPGAEQIAALTGPPAPLVAPTTAVGASAPSKSRRDGGVAGTPRRGPR
                     RAARRRSTRRAA"
BASE COUNT          546 a         1078 c         1067 g          559 t
ORIGIN      
        1 ccgcgttgag gaaatattac ccgtggattg accaagacgc cttggtttac tttgactcgc
       61 ggataggcca tgctcgcctg gaatgcgcag acgcgctgga tatcgtgttg tcgcactgca
      121 cgtccaggga aggccaggac gccgccttgc atgcgctggc gttcaagacc gatgtgctgt
      181 ggagcatact cgacgcgatc gagcatgctc tgcttcgatg atggcggcgt tgtcgctcac
      241 cgaccggccg cagttgcgcc gcggggtgcg tgccgggacc gatccgctca gcggtgagac
      301 ggtgctgctg ttccctgagg gtgtgctcat gatgaacgag acggcggctg ccgtcgtccg
      361 gcactgcgac ggcaaccata gtgtggccga cgtgctgcag tcgattggcg aggcctacga
      421 cgacgtcgcg gccgacgatg tcatgtcgct gctccatgat ctgattgccc agggcctgct
      481 ggtggccggc gatggatagg ccgttgggct tgctcgccga gctcacctac cgttgcccgc
      541 tgcattgccc gtactgctcc aatcccgttg ctgtccccca agcagcggag ttggcactgc
      601 aggagtggct gggcgtgctg gacgaggctc ggcagctcgg cgtgctgcaa ctgcacctca
      661 ccgggggcga gccgctggcc cgtccggacc tgaccccgct tgtcgcacgg gcccgcgatc
      721 tcggtttcta cgtcaacctg gtcaccagtg gggtgggcct cgacgagcgg cgtgcgaccg
      781 agctcgcgca ggctgggctc gaccacgtcc aactcagcat ccaggatgcc gaccgcacgg
      841 cggccgacga ggtcgcaggt gcccgcgtct gggaccgcaa aatcgcggct gcccgggtgg
      901 tgacggcgtt ggcgctgccg ctgacgatca acgtcgtgct gcatcgtggg aacatcagcc
      961 ggatcggccc gctggtggag ctcgctgctg agctgggcgc cgaccggctc gagctcgcgc
     1021 atactcagta ttacggctgg gctttactca accggtcagc gctgctgccg acgcgcgaac
     1081 aggtcgtcgc tgccgaccac gccgtgaccc aagcgcgagc ggcgtacggt gacaccctcg
     1141 agatccttta cgtcgtcgcg gactaccacg agccctaccc gaaaccgtgc atgcacggct
     1201 ggggacggcg gcacatcatc atcacacccg acgggcgggt gttgccgtgc ccggccgcag
     1261 ggcagatcac cgggctggac atcgacaacg tgcgagagca ctcactgcgc acgatctggc
     1321 agcactccgc ggctttcacc cggttccggg gatcggggtg gatggctgag ccgtgccgca
     1381 gttgcccgcg caaagaggtc gacttcggcg gctgccggtg ccaggccttc cagctcaccg
     1441 gtgacgccgc ccgggccgac ccggtgtgcc aactgtctgc cgaccacgac ctgatccgcg
     1501 cggtcgtcga tgccaccgac ccgcaacagc cgctgcgccc acgggtaaat ccggatcgac
     1561 aacggcccca ccagtaaagt cgcctactgg cgacaggagt tgtgacgtac tgtcatcgcc
     1621 aagtctttta aatgttaccc cgattttatt gcgagggtgg ttgcacgctg cgccgatcac
     1681 gaggagggtg gtacgtggac ggcttatacg gcatccccga gacccgggag caccaataca
     1741 tgctcacgca tattgaagac gaccaacgag tcgccgacgt tggcgacgac cgaggcacgc
     1801 gcccggtcca tgacgccgca ggtccgatca cccagcgcgc gtacgactcg atggcccagc
     1861 tcgtgcgtca aggactggac acctcactga gatcggtcca ggtgtgggcc gacctggctc
     1921 gccaggtcga ttccaccgcg gtgacctctg cttatgaccc gtttgagcag ttactggcaa
     1981 cccagcgcga gatcgtcggc gacctcgtag ccacccagcg ccaactcgcg cagcaggtgt
     2041 tcgacacctc cgccgccgcg ggcggcgccg ccgttccccg gtaggaatgg ccaagccagc
     2101 accgcagctg gatgatgcta tccgcgcatc gtaggggcat gttgataggc caagtcggat
     2161 gctatgcgcg ctcgatactt taccccagta actctttacg tggatataac gagcggtatc
     2221 ctccgatcgg tgcaatggtt gctccaccaa ggggatgact atattcgtca tcactgcgcc
     2281 ggagtggccg cattaactcg cgcctaatga atcagccgac tacaccgtcg agctgcgggc
     2341 tgaaaacaac gactaaccag aaggtggttt cgcgatgagc aactcgctgc aactagccca
     2401 ccagtggtgg gccgctctcg gacaggccga ccgtcagcgg gtctgggaca gccgccacag
     2461 ttacctcgct ggcgacctgg tggaaagcat ggccaaagcc ggcgtgcccg tcgtcagtga
     2521 cggccgctgg tcctgtgtca gcatcggacc caccggcttc accgtgccca ccgccgtcga
     2581 gcaactcctc aagggattat cccggactcc agacggagac cgcccactcg gcaacgcata
     2641 ggtacggcaa tctcgtgggt tggcccatcc cgccagccga ggccgcccag ctggaactcg
     2701 cccgccggtg gctcgccgcc gtgagcttac tcaggcggcg cggcgagtag agcggcgccg
     2761 ggcggctcgc cgtggtccgc gacgaggagt cccggcaacg ccgccgtctc ggcggctctt
     2821 ggatggcgcc gacgcgccaa cagcagtcgt cggggcgacc agtggagccg gtgggccggt
     2881 gagtgcggcg atctgttcgg caccgggacg cactcgcgtg gtcaccggcc tgatgccggc
     2941 ctggcgagtc agtgtccgca tttccccgga ttggtcgggg gtggacaggg tgacgacgat
     3001 cccctcggcg cccgccctgg cagtgcgccc ggaacggtgc aggtaaccct tgtactcggc
     3061 gggcggatcg acatggatca ccaggccaat ctcatcgacg tggatgccgc gcgcggcgat
     3121 gtccgtcgcc accaataccc gcgcactacc ctcgctgaac gccgcaagat tgcgtgccct
     3181 ggcgttctgc gagaggttgc cgtgcagctc cacggcgggc agcccggcgg cggtcagctg
     3241 cttggcgagc
//
LOCUS       DATADN010000701         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_227646, whole genome shotgun sequence.
ACCESSION   DATADN010000701 DATADN010000000
VERSION     DATADN010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227646"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /gene="speA"
                     /locus_tag="VHH53_09065"
     CDS             complement(<1..1632)
                     /gene="speA"
                     /locus_tag="VHH53_09065"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX2290327.1"
                     /translation="MPLLVRFSDILRRRVEQLCGSFQQAIAENDYKGSYRGVYPIKVN
                     QQRHVVEEIVEFGRPFGVGLECGSKPELQAVLALNERTDHLIICNGYKDEEFMRLALM
                     GQKLGHQVLIVIEKLSEVEVLLRVAEEMGVEPTAGVRIKLSATGAGRWSETAGEKSKF
                     GLNSAQLVRVIDMLENAGKLDALKLIHFHLGSQIPDIRNIKLAMTEVARYYVELRQLG
                     VGVDYVDVGGGLGVDYDGSRSTSSASVNYSMQEYANDIIYALAEACRENEVPMPHVIS
                     ESGRALTAHHALLLINVIDLETQSPEKPGPVEEDDHALVHELAGVLRELDARRVHEVY
                     HDATFLKEQLVVHFNSGTLSLRERANGERYWLAIMNRVAQLAAKDPEEYEDILPELEG
                     ILIDRYFCNFSLFQSLPDSWAIDQLFPIMPVHRLDEEPTRRGTLQDVTCDSDGKIDHF
                     VGWRKSKPSLELHAFRHGEPYVLGIFLTGAYQEILGDLHNLFGDTNAVHVRLTEQGYE
                     ITDMVHGDTVTEVLNYVQFHASDLLATFRRKVGNAK"
BASE COUNT          236 a          555 c          545 g          304 t
ORIGIN      
        1 cttggcgttg ccgaccttgc gccgaaaggt ggccagcagg tcggacgcgt ggaactgcac
       61 gtagttgagg acctcggtca ccgtgtcccc gtggaccatg tcggtgatct cgtagccctg
      121 ctcggtgagc cgcacgtgca cggcgttggt gtcgccgaac aggttgtgca ggtcgcccag
      181 gatctcctgg tacgcgccgg tgaggaagat gcccagcacg tacggctcgc cgtggcgaaa
      241 ggcgtgcagc tccaggctgg gcttgctctt tcgccacccc acgaagtggt cgatcttgcc
      301 gtcgctgtcg caggtcacgt cctgcagcgt gccgcggcgg gtgggctcct cgtccagccg
      361 gtggaccggc atgatgggga agagctggtc gatggcccag ctgtccggca gcgactggaa
      421 gagcgagaag ttgcagaagt agcggtcgat caggatcccc tccagctcgg ggaggatgtc
      481 ctcgtactcc tccgggtcct tggccgccag ctgcgccacc cggttcatga tggccagcca
      541 gtagcgctcg ccgttggccc gctcgcgcag gctgagcgtc cccgagttga agtggacgac
      601 cagctgctcc ttgaggaagg tggcgtcgtg gtacacctcg tgtacgcggc gcgcgtccag
      661 ctcgcgcagc acgccggcca gctcgtggac gagcgcgtgg tcgtcctcct ccaccggccc
      721 cggcttttcc gggctctgcg tctccaggtc gatgacgttg atcagcagca gcgcgtggtg
      781 cgcggtgagc gcccggccgc tctcgctgat cacgtgcggc atgggcacct cgttctcgcg
      841 gcacgcctcg gccagggcgt agatgatgtc gttggcgtac tcctgcatgg agtagttcac
      901 gctggccgag ctggtcgacc gcgacccgtc gtagtccacc cccagcccgc ctccgacgtc
      961 cacgtaatcg acgccgacgc ccagctggcg cagctccacg tagtaccggg ccacctccgt
     1021 catcgccagc ttgatgttgc ggatgtcggg gatctgcgag cccaggtgaa agtggatgag
     1081 cttgagcgcg tccagcttgc ccgcattctc cagcatgtcg atgacccgta ccagctgggc
     1141 gctgttcagg ccgaacttgc tcttttcccc cgcggtctcg ctccagcggc cggcgccggt
     1201 ggcgctgagc ttgatgcgca cgccggcggt gggctccacc cccatctcct ccgccacccg
     1261 cagcagcacc tccacctcgc tcagcttttc gatgacgatg agcacctggt ggcccagctt
     1321 ctggcccatc agggccagcc gcatgaactc ctcgtccttg tagccgttgc agatgatcag
     1381 gtggtccgtg cgctcgttga gcgccagcac cgcctgcagc tccggcttgg agccgcactc
     1441 cagccccacg ccgaaggggc ggccgaactc cacgatctcc tcgacgacgt ggcgctgctg
     1501 gttgaccttg atggggtaga cgccgcggta gctgcccttg tagtcgttct cggcgatcgc
     1561 ctgctggaac gagccgcaga gctgctcgac gcggcggcgc aggatgtcgg agaagcgcac
     1621 gagcagcggc agcttgaggc
//
LOCUS       DATADN010000702         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1280846, whole genome shotgun sequence.
ACCESSION   DATADN010000702 DATADN010000000
VERSION     DATADN010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280846"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VHH53_09070"
     CDS             <1..364
                     /locus_tag="VHH53_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290328.1"
                     /translation="PFQIARHRGAKLGAAERERARPGRLSAERALTLATAILTRYGFE
                     PDRVTPTLLRLRNCPFAPLPAKAPQLVCGINHVFLTGFLNTLQASTLTAVLTPRPNGC
                     CVELTPLTRTQPSGSSIA"
     gene            683..1057
                     /locus_tag="VHH53_09075"
     CDS             683..1057
                     /locus_tag="VHH53_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2290329.1"
                     /translation="MTGNKCGHGSDGVVVLGFVVGGGSAAGSSSVGGGRWVAVTDLAD
                     TVVIKDFAFGPTSLVVAPETKITVVKQDRAPHTVTARDNSFDNGTIAGGQRAEITAPS
                     SPGTYPYICAIHLSMTGTLLVK"
     gene            1276..2127
                     /locus_tag="VHH53_09080"
     CDS             1276..2127
                     /locus_tag="VHH53_09080"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2290330.1"
                     /translation="MPSPRELVRRIEHFTALDTVAEPVRGLVSKAVQPRLVRNLLSGT
                     NLGHPVHPMLTDVPIGAWSMSALLDTVGGASAEPAADLLVGAGILSAVPTAMTGLNDW
                     SDTIGEETRLGVMHAGANVAALGLYTASLLARRSGRRGVGKALGLAGLGVLMVGGYLG
                     GHLSFGRAVNVNHTAFEHGPQEWTPVLADAELDEGEHRRVSADGASVLLARDGGQLYA
                     LANTCTHAGGPLDEGELIDGCVICPWHASTFRLTDGKIVRGPASTPQPTYQIRVREGK
                     IEVRSSG"
BASE COUNT          440 a          802 c          791 g          459 t
ORIGIN      
        1 accctttcag atcgcccgcc accgcggtgc caagctaggt gccgccgaac gcgagcgagc
       61 ccggccaggg cgcctaagcg ccgaacgcgc gctgaccctg gccaccgcaa tcctcacccg
      121 atacggcttt gaacccgacc gggtcactcc cacgctgctg cgattgcgca actgcccgtt
      181 tgccccactc cccgccaagg ctccgcagct ggtctgcggt atcaaccacg tctttctcac
      241 cggcttcctc aacaccctgc aggcgtccac cctcaccgcc gtgctgactc cccgccccaa
      301 cgggtgctgc gtcgaactca caccgctaac ccgtacccag ccatccggca gttctattgc
      361 gtgagcgcat tgctgccaca aaggtgagtg acttctgtcc ccagcgtgct gcgcctacct
      421 cccgcggtcc ccactcaacg ccaaggtgga tacggcggga gacgggtcga ctcttcaagc
      481 gcggcgaccg cagagagctg gacggtgcaa gatccgttct aacacacact cctgttgacg
      541 aaacggcacc acagttctaa cgttagcgta tcatggcgct agaaggagct gggtagtgag
      601 aattgatcgg gtctgctcaa gaccctagga gagatggagg gggatgatat gaggatcatg
      661 gaaatcttcg aacttggcgg ctatgaccgg gaataagtgt ggtcatggta gtgacggggt
      721 cgtcgtgctg gggttcgtgg tgggcggcgg cagtgccgcg ggcagcagca gtgtcggggg
      781 cggcagatgg gtcgccgtca ctgacctggc cgacaccgtt gtgatcaaag atttcgcgtt
      841 cggaccgacc tctttagtgg tcgccccgga aacgaagatc acggtggtga aacaggatcg
      901 ggctccgcat accgtgactg cccgggacaa ctcattcgac aacggcacta tcgccggtgg
      961 gcagcgcgcc gaaattactg cgcccagctc gccgggcaca tacccttaca tctgcgctat
     1021 ccacctgtcc atgaccggca cactgctcgt gaaatgagcc agccgtaccc gacccgacgc
     1081 ccatcgcgcc gggcgacatc ccagatgggg gccgtcgctg gacggacacc gaccttacgc
     1141 gctcggtatc gccctgttcc atatccggaa tggaggaacg gggcacaccc gacgcgccgc
     1201 gatgtcctga gggtgcagtc catactgccc cggctacaca acgtccccgg cctaatgtga
     1261 aaggattttg ctgtcatgcc ctctccgcgt gagctagtgc gccgcattga acatttcacc
     1321 gccttggaca ccgtcgccga gccggtgcgc ggcttggtga gcaaggctgt gcagcctcgc
     1381 ttggtccgta acctgctcag tggtacgaat ttgggtcatc cggtccatcc catgctgacc
     1441 gacgtgccga tcggtgcctg gagcatgtct gcgttgttgg acaccgtggg cggggccagc
     1501 gctgagccag ctgccgacct gctcgtcggg gccggcatcc tcagcgcggt ccctaccgcc
     1561 atgaccgggc tcaacgactg gtcggacacc atcggcgagg agacgcgtct cggggtgatg
     1621 cacgccggcg caaacgtggc cgcgctcggt ttatacacag cctcgctgct cgcccgtcgc
     1681 agtggtcggc gtggtgtggg caaggcactg gggctcgctg ggctcggggt gctgatggtc
     1741 ggtgggtacc tcggtggtca cctgtcgttt ggcagggcgg tcaacgtcaa ccacaccgct
     1801 ttcgagcatg gcccgcagga gtggaccccg gtgctcgccg acgcggagct ggatgaaggt
     1861 gagcatcgcc gggtctccgc cgatggcgcc tcggtgctac tcgcccgcga tggcggccag
     1921 ctgtacgcgc tggccaacac ctgcacccac gccggcggtc cactggatga gggcgagctc
     1981 atcgatggct gcgttatctg cccgtggcac gccagcacct tccggctcac cgacggcaaa
     2041 atcgtgcgcg gcccagcctc gacaccccaa cccacctacc aaatccgcgt ccgtgaggga
     2101 aaaatcgagg tccggtcctc aggctgagca ctgcatacgg cctgggacag cgatgccacc
     2161 accaagggtc ccgcgcggtt cgatgcgtgg tgtcaaggga gatccgcagt cagcggcatt
     2221 tctgtcctgc aggttggtgt aactgtgacg ctgatgcggt catgtccggt gttgctgagg
     2281 gacagtgggc tcgaggcaaa tgcagcagtg gggtggattg ggtccaggtg cgcggtcagc
     2341 ccagtggtag ccaggccgtc ggaatgcgcc gaaccggaag gatcgctgcc ctcctctgtc
     2401 agcccactag gagcacgaag cctctttcac ggcatgccct cgtttattcg tgaaggctca
     2461 ctttaggtgt ggagaggggt cttggatgga tg
//
LOCUS       DATADN010000703         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_690193, whole genome shotgun sequence.
ACCESSION   DATADN010000703 DATADN010000000
VERSION     DATADN010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690193"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..740)
                     /locus_tag="VHH53_09085"
     CDS             complement(102..740)
                     /locus_tag="VHH53_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290331.1"
                     /translation="MDREVRQELLTLPKGLADLVGSHLVAAGRALDEDPQLALAHARF
                     ARSRAGRVAAVREAAGLAAYHAGEWSEALAELRAARRLGGGPGRLAVLADCERALGRP
                     ERALEIARSPEAAQLSTDEAVELRMVAAGARRDLGQFDAAVVALQGPDLEVAGPEPWR
                     ARLRYAYADNLLAAGREQDAIRWFLAAAEVDADDATDAGERAIELSERSDPQ"
     gene            complement(1017..1133)
                     /gene="rrf"
                     /locus_tag="VHH53_09090"
     rRNA            complement(1017..1133)
                     /gene="rrf"
                     /locus_tag="VHH53_09090"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1229..>1549)
                     /locus_tag="VHH53_09095"
     rRNA            complement(1229..>1549)
                     /locus_tag="VHH53_09095"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          231 a          552 c          483 g          283 t
ORIGIN      
        1 ctcgttgacc acggccggcg cctccggtac aacctcggct ccgcgcacaa cggtgccgtc
       61 aagatcgaga agtacggcgt cgtagccctc cagcggagcg ctcactgcgg atccgagcgc
      121 tcgctgagct cgatggcccg ctccccggca tcggtcgcat cgtcggcatc gacctcggcg
      181 gcagccagga accagcggat cgcgtcctgc tcgcggccgg cagccagcag gttatccgca
      241 tatgcatagc gcaacctggc ccgccacggc tccgggcccg ccacctccaa gtccgggccc
      301 tggagtgcaa caaccgcagc gtcgaactgg ccaaggtcgc gccgcgctcc ggcggcgacc
      361 atccgcagct cgaccgcttc atcagtgctg agctgcgctg cttctggact gcgggcaatt
      421 tccaatgcgc gttccggacg tccgagcgcg cgctcgcagt cggctagcac cgccagccgc
      481 cccggaccgc cgcccagccg gcgcgcagcg cgaagctcgg ctaacgcctc tgaccattca
      541 cccgcgtgat aggcggcgag acctgccgcc tcccggactg cggcgacccg cccagctcgg
      601 ctacgggcaa accgggcatg cgccaaggcc agctgcgggt cctcgtccag cgcgcgaccg
      661 gccgccacca ggtggctacc gacgaggtcg gccaaccctt tcggcagggt caacagttcc
      721 tggcgaacct cccgatccaa ctcactagct tctacgtctg ggggaagttc cggacggggt
      781 gggtcgactt cttcgtcgcg gcgccgagac ggcgcacggc cgccgtcctc gggtcggcct
      841 ccccgatttc ccgttcggcc cctgccccct ccagggctgc gcccacgacg ctctccacgg
      901 tcccggcccg cacctggagt cagattcatt ccaactggca cggggacaaa tcctctcatc
      961 gctcgcaaca ctaaggggcc cccgggtggt ttgggggccc cttagtgggg gtttgtgttc
     1021 ggcggtgtcc tactctccca caccctggcg ggtgcagtac catcggcgct ggagggctta
     1081 gcttccgggt tcggaatggg gccgggcgtt tcccctccgc tatggccgcg gtaacttgtg
     1141 tggagttgtg tggtcggtgg tgtcgtgtgg tgacaccgat gccggccgtg ctccaagagc
     1201 tgcacagtgg atgcgttgtt tgcgtgtttg tggtaagtcc tcggcctatt agtaccggtc
     1261 agctgcacac cttgcggtgc ttccacttcc ggcctatcaa cccagtggtc tagctggggg
     1321 ccttaaccca cggtggggtg ggagacctca tcttggaacg agcttcccgc ttagatgctt
     1381 tcagcggtta tcccttccga acgtggctaa ccagccgtgc ccctggcggg acaactggca
     1441 caccagaggt tcgtccgtcc cggtcctctc gtactaggga cagccttcct caagtctcct
     1501 gcgcgcgcgg cggataggga ccgaactgtc tcacgacgtt ctaaaccca
//
LOCUS       DATADN010000704         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1449999, whole genome shotgun sequence.
ACCESSION   DATADN010000704 DATADN010000000
VERSION     DATADN010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449999"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VHH53_09100"
     CDS             complement(<1..586)
                     /locus_tag="VHH53_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2290332.1"
                     /translation="MNEPVPWIEDRWPVGSVQVRSIVLVLHGGQARSSTTVQRRTQLS
                     YLRMVPFARFLHRRDGGRGLSVRILRYRYRGWDEPERHPVQDASWALAQLRGQHPEAS
                     IALLGHSMGGRAALRVADHAAVTAVCALAPWTMDDEPVRQLAGRSVLIAHGDREHTTD
                     PRLSYRFAVRAKQVTSRVCRFDVLGDGHAMLRRAH"
     gene            complement(600..1274)
                     /locus_tag="VHH53_09105"
     CDS             complement(600..1274)
                     /locus_tag="VHH53_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2290333.1"
                     /translation="MAGDVVHAVIFDLDGVLVDSEELWDVVRRDVAAEAGRPWPPEAT
                     RAMQGMSTAEWSAYLTDAVGVPGEPDEVAMTVIDRMAAQYEASLPLLPGAVEVVERLG
                     RRWPLGLASSSPRRLIDTVLDSAGLAQRFHVSVSTEEVSAGKPSPAVYHTVVQRLEVN
                     PLQAIAIEDSSNGLRSAATAGLGVLAVPNVAFPPSEDALALADVVVDSLDEITLELVA
                     SMGARG"
     gene            complement(1384..1725)
                     /locus_tag="VHH53_09110"
     CDS             complement(1384..1725)
                     /locus_tag="VHH53_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEX2290334.1"
                     /translation="MPETKLHPSCVTDLKAPIFDEDWQRRAFGLAVALSEFGHYPWDD
                     FQQALITAIGSWQEEPTNARGRWEYYQHWVAALNTVVRQHGLLDDGYVNPEDRLTSSL
                     PPDTKRRSGVL"
BASE COUNT          310 a          655 c          572 g          284 t
ORIGIN      
        1 cgtgcgcgcg gcggagcatc gcgtgcccat ctccgagcac gtcgaaacgg cagacccggg
       61 acgtgacctg cttggcgcgc accgcaaacc ggtaggacag ccgcggatca gtggtgtgct
      121 cccggtcacc atgcgcgatg agcaccgacc gcccggcgag ctgccgcacc ggctcatcgt
      181 ccatggtcca cggcgctagc gcacacactg cggtcaccgc cgcatggtcc gctacccgca
      241 gcgccgcccg ccctcccatc gagtgtccca acagcgctat cgatgcttcc gggtgctggc
      301 cccgaagctg cgccaatgcc cagctcgcgt cctgcacagg gtggcgttcg ggctcgtccc
      361 atccccggta gcgataccgc aggatgcgta ccgacagccc gcgcccgccg tcacgccggt
      421 gtaggaaccg agcgaagggc accatccgga gatagctcaa ctgcgtccgc cgctggaccg
      481 tggtggagct ccgggcctgt cccccgtgca acaccagcac gatggaacgg acctgcaccg
      541 agcccaccgg ccagcggtcc tcgatccagg gtacgggttc gttcacgggt ctcaactcgc
      601 taaccccggg cgcccatcga cgccaccagc tccagggtga tctcgtcgag cgagtccacg
      661 acgacgtcgg caagcgcaag cgcatcctcg gagggcggaa aggccacatt gggcacggcg
      721 agcaccccca gaccggcggt agcggccgac cgcagtccgt tactcgaatc ctcgatcgct
      781 atcgcctgca gcggattcac ctcgagtcgc tgtaccacgg tgtgataaac cgcaggcgag
      841 ggctttccag cgcttacctc ctccgtggac acactgacgt gaaaccgctg cgctaaccct
      901 gccgagtcca gcaccgtgtc gatcagccgc cgcggtgagg acgacgcgag cccgagcggc
      961 cagcggcgcc ccagccgctc gacgacctcg accgcacccg ggagtagagg tagcgatgct
     1021 tcgtactgag cggccatccg gtcgatcacg gtcatcgcca cctcgtccgg ttcaccgggt
     1081 acgcccaccg catcagtcag gtacgccgac cactccgccg tgctcatacc ctgcatcgcc
     1141 cgggtcgcct ccgggggcca gggccggccg gcttcggcgg caacatcgcg gcggacgacg
     1201 tcccacaact cctccgaatc cacaagcacg ccgtcgaggt cgaagatcac cgcgtgcacc
     1261 acgtccccgg ccatcctgcc tccacgagtt gcctccgaca gttccgtacg attctcaccg
     1321 aacggctgcc cattgggtcg ctcacggtgg cgccgagcgg cctgagagag acggtgcgag
     1381 cgcttacaag acgcccgatc tgcgtttcgt gtcaggtggg agggaagagg taaggcggtc
     1441 ttcagggttg acgtagccgt cgtcgagaag gccgtgttgt cgcaccacag tgttcagagc
     1501 ggcaacccag tgctggtagt actcccaccg gccgcgggcg ttggtgggtt cctcctgcca
     1561 tgagccgatg gcggtgatca gcgcctgctg gaagtcgtcc cacgggtagt ggccgaactc
     1621 cgacaacgcg acggccagcc cgaacgcccg gcgctgccag tcctcgtcga agatcggcgc
     1681 cttgaggtcg gtgacgcacg acgggtgcag tttcgtctcc ggcatggatt cagctctccg
     1741 caggtacttc ggcgagcgca acgccgatca tcgactcggt cgtcacgagt tgggcgagtt
     1801 gctcctcgga catcccttcg g
//
LOCUS       DATADN010000705         4056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1450009, whole genome shotgun sequence.
ACCESSION   DATADN010000705 DATADN010000000
VERSION     DATADN010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450009"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VHH53_09115"
     CDS             complement(<1..220)
                     /locus_tag="VHH53_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290335.1"
                     /translation="MTNQPPRTVDDPRSRVDDPRLQVPSANDGLPRRDADGDKQGSSD
                     LRANAGRHPAGWPLSEAAYRLAHRTRLAD"
     gene            289..1227
                     /locus_tag="VHH53_09120"
     CDS             289..1227
                     /locus_tag="VHH53_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX2290336.1"
                     /translation="MSRVAAIDCGTNSIRLLVANVDEGRLRDVHREMRIVRLGQDVDA
                     TGRLAPEALERTRVALADYATIAMRAGAERTRMVATSATRDAANRADFFTMVRQTLGT
                     DAEVITGDEEARLSFTGAVGDLDPGDGPFVVVDVGGGSTEVVLGDWDGSRADVTAARS
                     VNVGCVRITERHLRSDPATPDEVSAAEVFAAQMLQEAFADVPVEKARTWVGVAGTVTT
                     LSAVAQQLPAYDPERTHLSRLPLKQLRATTEALLASTHRQRATNPAIHPGRVDVIAGG
                     ALIVRTLAEELHVRAGIGELVVSEHDILDGIALGLS"
     gene            1241..1978
                     /locus_tag="VHH53_09125"
     CDS             1241..1978
                     /locus_tag="VHH53_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2290337.1"
                     /translation="MRARHTVHESLTCGGLGALDEAVTRCRACPRLVAWREQVAVEKR
                     AAFRDQTYWGRPVPGFGPADAALAIVGLAPAAHGGNRTGRIFTGDRSGDVLYAALHAV
                     GLANQANATHRGDGLTLHRTRITAPVHCAPPANKPTPAERDACRPWLVRELQLLRPTL
                     CAVVVLGGFGWQALLPALVTAGWLVPTPLPRFGHGTEVNLDGLHLLGCYHVSQQNTFT
                     GRLTPAMLVDVLRRAAELAGISCTERA"
     gene            2045..3367
                     /locus_tag="VHH53_09130"
     CDS             2045..3367
                     /locus_tag="VHH53_09130"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2290338.1"
                     /translation="MAFTAGEPARVLIVGGGHVGLYTALRLQAKLRTDEASVTVVDPQ
                     TQMTYQPFLPEAAAGSIEPRHVVVPLRRVLRRCHTVCGRVTGVDHARRFATIENCAGH
                     VEQLGYDVLVMAPGSIARTLPIPGLAEHGIAFKTIGEAIYLRNHVLSCLEAADATLDP
                     RLRRELLSFVVIGGGYAGIEVLAELEDMARYACRYYETIQPQDMRWVLIEATGRIMPE
                     VSPKMGRYTAKRLIHRGIEIFLNTRVTTMQEGHVVLDNGTEFDADTIVWTAGVKANPV
                     LQRTDLPLDDRKRLRCTTTLQVVNCPDVFSAGDCAAVPDLTSNDPNATTSPSAQHAVR
                     QAKVLADNIIAQLRGEPLQHYKHRYAGSVASLGLYRGVAEIYGVRLRGIAAWFMHRTY
                     HLSRMPTFNRKVRIVADWTAALLFHREVVALGQINDPKADFVRATTAS"
     gene            3420..3493
                     /locus_tag="VHH53_09135"
     tRNA            3420..3493
                     /locus_tag="VHH53_09135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3454..3456,aa:Leu,seq:taa)
     gene            complement(3630..>4056)
                     /locus_tag="VHH53_09140"
     CDS             complement(3630..>4056)
                     /locus_tag="VHH53_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2290339.1"
                     /translation="RRDQRQLKHRAKGDTRSAPSPPELTQLLHDHLALHGTDAEGRLF
                     RGVRSGRELEESTYHRVWRKARQAALTAEEYTSPLARRPYDLRHAAVSTWLNAGVPAT
                     QVAEWAGHSVAVLLQIYAKCLSGQEELARKRIEARLREA"
BASE COUNT          632 a         1363 c         1382 g          679 t
ORIGIN      
        1 agtcggccag ccgcgtgcgg tgcgccagac gataggcggc ctcggacagg ggccaccccg
       61 cagggtggcg gccagcgttg gcccgcaggt ccgaactgcc ctgcttgtcg ccatcggcat
      121 cccggcgagg gaggccgtcg ttggccgacg gcacctgcaa cctcgggtcg tctacccgcg
      181 agcgtggatc atcgacagtg cggggcggct ggttggtcaa ggccaggtac ttgtcgtcaa
      241 tgttcctgat cagcgtacgg ttggccgcta cgagcctcgg aggtggatat gtccagggtg
      301 gccgcgatcg attgcgggac caactcgatc cgcctgctcg tcgccaacgt cgacgaggga
      361 cggctgcgcg acgtgcaccg cgagatgcgc attgtccggc tcggtcagga cgttgacgcc
      421 actggtcgac tggctccgga ggcccttgag cgcacccgcg tcgcgctggc cgactacgca
      481 accatcgcca tgcgcgccgg ggcagaacgg acccggatgg tggccacgtc ggcgacccgt
      541 gacgccgcca accgggcgga ctttttcacg atggtccgcc agacgctcgg caccgatgcc
      601 gaggtgatca ccggggacga ggaggcccgg ctgtcgttca ccggcgcggt cggtgacctc
      661 gatcctggtg atgggccgtt cgtggtcgtt gatgtcggtg gtggatccac cgaggtggtg
      721 ttgggggact gggacgggtc gcgagccgac gtgacggcgg cgcggtcagt gaacgtgggc
      781 tgcgtgcgga tcaccgagcg gcacctgcgc tccgatcccg cgacgccgga tgaggttagc
      841 gctgccgagg tgttcgcggc gcagatgctg caggaggctt tcgccgacgt gccggtggag
      901 aaggctcgca cgtgggtcgg ggtggcaggc accgttacca ccctgtccgc cgtcgcgcag
      961 cagttgcccg cctacgatcc tgaacgcacc cacctgtccc ggctgccgtt gaagcagctg
     1021 cgcgccacga ctgaggcgct gctcgcgtcg acgcatcgcc agcgggcgac caaccccgcc
     1081 atccaccccg gccgagtgga cgtcatcgcc ggcggggcgc tcatcgttcg gacgctcgcc
     1141 gaggagctgc acgttcgtgc tggcatcggt gaactggtgg tcagtgagca cgacatcctt
     1201 gacggcatcg cgctgggtct cagttgaggg cctggtgtgc atgagagcgc gacacacggt
     1261 gcatgagagc ctgacatgcg ggggattggg cgcgttggat gaggcggtga cccggtgccg
     1321 ggcgtgcccg cgcttggtgg cctggcggga gcaggtcgcg gtcgagaagc gcgctgcatt
     1381 ccgcgaccag acctactggg gacgtccagt ccccggcttc ggtccagccg acgccgcgct
     1441 ggccatcgtc gggcttgccc cggccgcgca cggtggcaac cggaccggcc ggatattcac
     1501 cggggatcgc tctggtgatg tgctctacgc tgcgctgcat gccgtcggct tggctaacca
     1561 agccaacgcc acccaccgcg gcgacgggct gacgctgcac cgcacccgaa tcacagcccc
     1621 ggtgcactgc gcgcccccgg cgaacaagcc aaccccggcc gagcgcgacg cctgcaggcc
     1681 ctggctggtt cgggagttgc agctgctgcg cccgacgctg tgcgcagtag tcgtgctggg
     1741 tggtttcgga tggcaggcgc tgctgcccgc gctggtcacc gcaggctggc tggttcccac
     1801 cccgctgccg cgtttcggac acggcaccga agtgaacctg gacgggctgc atctgctcgg
     1861 ttgctaccac gtcagccagc agaacacctt caccggacgg ctcactccgg cgatgctcgt
     1921 cgacgtgctg cgccgcgcgg ccgaactggc cgggatcagc tgcaccgaac gcgcctgacc
     1981 cggcgaaaca gtaatccttt attggtgacg cgcatggcgg cagctgcggt gggaacactg
     2041 tgatatggca tttacagctg gcgagccggc gcgcgtgctc atcgtcggcg gtggccacgt
     2101 ggggctgtac accgcgctgc gactgcaggc gaagctgcgc acggatgagg cctcggtcac
     2161 cgtggtcgat ccccagaccc agatgaccta ccagccgttc ctgcccgagg ccgccgccgg
     2221 atcgatcgaa cctcgccacg tggtggtgcc gctgcgccgg gtgcttcggc gctgccacac
     2281 cgtctgtggc cgggtcaccg gtgtcgacca cgcccgccga ttcgccacca tcgagaactg
     2341 cgccgggcac gtcgagcaac tcggctatga cgtgctggtg atggcgccgg gctccatcgc
     2401 gcgcacgttg cccatccccg ggttggccga gcacggcatt gcgttcaaga ccatcggtga
     2461 agcgatctac cttcgtaacc acgtgctgtc gtgtctggag gctgcggacg ccacgctgga
     2521 tccacgactg cgccgcgagc tgctgagctt cgtggtgatc ggtggaggat atgcgggcat
     2581 cgaggtgctg gccgagctcg aggacatggc ccgctacgcc tgccgctact acgagaccat
     2641 ccagccgcag gacatgcgct gggtactcat cgaggccacc ggcaggatca tgcccgaggt
     2701 cagcccgaaa atgggtcgct acaccgccaa gcggttgatc caccgcggta tcgagatctt
     2761 cctgaatacc cgggtcacca cgatgcaaga aggccacgtc gtcctcgaca acggcacgga
     2821 gttcgacgcc gacaccatcg tctggaccgc tggggtgaaa gccaaccccg tgttgcagcg
     2881 aaccgacctg ccactggacg atcgcaagcg gctgcgctgc acgaccacct tgcaggtagt
     2941 gaactgcccg gacgtgttct ccgccgggga ctgcgcggcg gtgcccgacc tcacctccaa
     3001 cgaccctaac gccaccacca gcccgtcggc ccagcatgct gtgcgacagg ccaaggtgct
     3061 ggctgacaac atcatcgcgc aactgcgcgg cgagccgttg cagcactaca agcaccgtta
     3121 cgcaggatcg gtggccagtc tgggcctcta ccgcggcgtc gccgagatct acggggtaag
     3181 gctgcggggc atcgcggcct ggttcatgca ccgcacctac catctgagcc ggatgccgac
     3241 gttcaaccgc aaggttcgca tcgtggccga ctggaccgct gccctactgt ttcaccgcga
     3301 ggtggtggcg ttgggtcaga tcaatgaccc gaaagcggac ttcgtgcgcg ccaccaccgc
     3361 ttcttgatct accccaggtt ggcggggatc cagggtgacg cggctacgct gcgcgaagcg
     3421 cccccgtagc ccaaccggca gaggcaggtc ccttaaaagg atcccagtgt gggttcgact
     3481 cccaccgggg gcacccgccc ttaccactcg catcgtctcc tgacctgcgc gaacgaggtc
     3541 aggagaccgt agggtgctgt ccggctgtgc cagctacggc cggtactcac cggccgtctg
     3601 tgccgaatac gtgccgaagt gcgcacctgc tacgcctcac gcaacctggc ctcgattcgt
     3661 ttccgagcca gttcctcctg ccccgagagg cacttcgcgt agatctgcag caggaccgcg
     3721 acactgtggc ctgcccactc cgcgacctgc gtggccggca cgcctgcgtt gagccatgtc
     3781 gacaccgccg cgtggcggag gtcgtaaggc cgccgcgcaa gcggcgaggt gtattcctcg
     3841 gcggtcagcg ctgcttggcg ggccttacgc cacacacggt gataggtgct ctcctccagc
     3901 tcgcgcccgc tgcggacccc tcgaaatagt cggccctcgg catcggttcc gtgcagggcc
     3961 aggtgatcgt gaagtagctg agtaagttct gggggagagg gagctgagcg ggtgtcgccc
     4021 ttggcccggt gcttgagttg ccgctggtct cgcctg
//
LOCUS       DATADN010000706         4169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1281393, whole genome shotgun sequence.
ACCESSION   DATADN010000706 DATADN010000000
VERSION     DATADN010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281393"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VHH53_09145"
     CDS             <1..777
                     /locus_tag="VHH53_09145"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HEX2290340.1"
                     /translation="GDPPHRALVDAFDDMLTSVGLPLVHAAWTAQITGGAPWRCYDDS
                     VCAGTVSDPGASPLAAATVAAGAVTFGSREEMVALLAGEDPAALQRRAVLLAADADHP
                     MSSALVAQRFAQLRELHQAAAAGDLTLSDKAVTEVASALCDHRVRDSCLPWCSGPGAV
                     AAERLWLALVRATPAPERAEPAALLALVAYLRGDGALAGVALDAALQACPQHSLSGLL
                     RAALDGGLPPEVLRTVAADAAAALPGPPRRNRKQRSRRKR"
     gene            complement(783..1736)
                     /gene="galE"
                     /locus_tag="VHH53_09150"
     CDS             complement(783..1736)
                     /gene="galE"
                     /locus_tag="VHH53_09150"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX2290341.1"
                     /translation="MRLLVTGGAGYIGSVCAARLVKAGHEVVVLDDLSTGHVDAVPAG
                     CRFVEGTVEDAGPVLAEGFDGVLHFAARSLVGESVTQPQRYWLGNMVASVRLLEAMRE
                     HGTPRLVFSSTAATYGEPASGPITEDAATLPTNPYGATKLAIDHAIGSYAAAHGLAAI
                     SLRYFNVAGAYGRYGERHTVETHLIPLVLQVALGHREKIMIFGQDWPTPDGTCIRDYI
                     HVVDLVDAHERALEQAEAGEHRIYNLGNGTGFSVQQVIDTCRQVTGHPIPAQSAARRP
                     GDPAVLIASSDRAREELGWKPERAELAAIIADAWNFARATS"
     gene            complement(1787..2482)
                     /locus_tag="VHH53_09155"
     CDS             complement(1787..2482)
                     /locus_tag="VHH53_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX2290342.1"
                     /translation="MNDLIDTTEMYLRTIFELEEEGVVPLRARIAERLGQSGPTVSQT
                     VARMERDGLLTVADDRHLELTAEGRNRATSVMRKHRLAERLLVDVIGLEWEQVHNEAC
                     RWEHVMSEAVERKLVALLGNPTTSPYGNPIPGLDELGIGEGAPPLEPDLVRLDEVARR
                     GGGTVEVRRIAENVQEDPALMADLHKVDAVPGNVVQVAALARAGHPVVLSGGGATVEV
                     DGMVAHAVLARRR"
     gene            2570..3418
                     /locus_tag="VHH53_09160"
     CDS             2570..3418
                     /locus_tag="VHH53_09160"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX2290343.1"
                     /translation="MHPLITPRQLAALLTDLEPPILVDVRWSLTGAPGCADYQAGHLP
                     GAVFLDMDTELAGSPGREGRHPLPDPAALQATLRAAGVRTTAPVVAYDAADGSVAARL
                     WWLLRWAGHSAVAVLDGGIAAWRAAGLPVTTEVPEPVASTITVRPPAMPVLDAAQAAA
                     LARNGVLLDARVPPRYRGEIEPVDPRAGHVPGAVNAPFDELTDADGRWLTPRRLRGLA
                     QRWGVESTEIGAYCGSGVNACALVLGLELAGITSPERPAGLYVGSWSQWCTDRDRPVA
                     TGPSPG"
     gene            3467..>4169
                     /locus_tag="VHH53_09165"
     CDS             3467..>4169
                     /locus_tag="VHH53_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX2290344.1"
                     /translation="MSAARAAVVWDEAFLGYDLGGWHPLNPIRLDLTIRLARSLGVLD
                     GVSLLTPVQATDEQLRMVHSDDYLAAVKNASRGYVGHGLGTSDNPVFRRMHEAAALVA
                     GGSLLAAEEILAGRAARVANIAGGLHHAMRDYASGFCIYNDCALAIRRLLDGGVSRVA
                     YVDIDVHHGDGVQEAFVDDPRVLTISLHQHPDTLWPGTGDSTDVGAPGAEGSAVNVPL
                     PPGTGDGEWLRAFHAV"
BASE COUNT          599 a         1412 c         1446 g          712 t
ORIGIN      
        1 ggtgatcccc cgcaccgcgc gttggtggat gccttcgacg acatgctgac cagcgttgga
       61 ctcccgctgg tacacgcggc ctggacggcg caaatcaccg gcggcgcccc gtggcgctgc
      121 tatgacgatt ccgtgtgcgc cggcaccgtg tccgatcccg gagccagccc gctggctgcc
      181 gctaccgtcg cggcgggtgc ggtgaccttc ggtagccgcg aggagatggt cgcgctactg
      241 gctggcgagg atcccgcagc tttgcaacgc cgggccgtgc tcctcgcggc cgacgctgac
      301 cacccgatga gctccgcgct ggtagcccag aggttcgcgc agttgcgaga attgcaccaa
      361 gcggcggccg cgggggacct cacgttgagt gacaaggctg tcaccgaggt cgcctccgcg
      421 ctgtgcgatc accgggttcg ggattcctgc ctgccctggt gctcgggacc gggcgcggtc
      481 gccgccgagc ggctctggct ggcccttgtt cgagcgaccc ccgcacccga gcgggccgag
      541 cctgcagcgc tgctggcgct ggtcgcttac cttcgcgggg acggtgcact ggccggcgtc
      601 gcgttggacg cggcgttgca ggcctgcccg cagcactcac tgtccgggtt gttacgagcc
      661 gcgctggatg gcggtctgcc acccgaggtg ctgcgtactg ttgccgcgga cgccgctgcg
      721 gcccttcccg gacctccgcg cagaaaccgt aagcaacgat cgcgacggaa gcgatgaccg
      781 ggtcagctgg tggcgcgggc gaagttccag gcgtcggcga tgatcgcggc gagttcagcc
      841 cgctccggtt tccagcccag ctcctcacgt gctcgatcgc tggaggcgat gagcactgcc
      901 ggatcgcccg gtcgtcgcgc cgcagactgc gccgggatgg ggtgtccggt gacctgccgg
      961 caggtgtcga tgacctgctg taccgagaag ccggtgccgt tgccgaggtt gtagatgcgg
     1021 tgttcgccgg cctcagcctg ctcgagcgcc cgttcatggg cgtcgacgag atctaccacg
     1081 tggatgtagt cgcggatgca ggtgccatcc ggggtaggcc agtcctgccc gaagatcatg
     1141 atcttttcac ggtgaccgag agcaacctgc agtacgaggg ggatgaggtg cgtctcgacg
     1201 gtgtgccgtt cgccgtaacg gccgtaggcg ccggcgacgt tgaagtaccg caagctgatg
     1261 gcggcgaggc cgtgcgcggc ggcgtaggag ccgatcgcat ggtcgatggc aagtttcgtc
     1321 gcaccgtagg gattggtggg cagggtggct gcatcctcgg tgatcggccc cgacgcgggt
     1381 tccccgtagg tggcggccgt ggaggaaaac accagccggg gagttccgtg ttcgcgcatc
     1441 gcctcgagca gccgcaccga ggcgaccata ttacccagcc agtaccgctg cggttgcgtc
     1501 accgattcac cgaccagcga gcgggccgcg aaatgcagca caccgtcgaa accctcagcc
     1561 agcacgggcc cggcgtcctc gacggtgccc tcgacgaacc ggcaaccggc gggtaccgca
     1621 tccacgtggc cggtggacag gtcatccagc accacgactt cgtgcccggc cttcaccagc
     1681 cgggcggcgc agacactgcc gatgtatccc gcacccccgg taaccagcag ccgcatcggc
     1741 tttggtccag tggttcccga cagcccagtg acttccgaca gcccagtcac cgccgacggg
     1801 cgagcactgc gtgcgcaacc atgccatcga cctctaccgt ggctcccccg ccggagagca
     1861 ctaccggatg gcctgcccgg gctaaggccg ccacctggac cacattgccg ggcaccgcgt
     1921 cgaccttgtg cagatcggcc atcagggccg gatcctcctg cacattctcg gcgatgcgtc
     1981 ggacctccac cgtgcccccg ccacgacggg ccacctcgtc gaggcgcacc agatcgggtt
     2041 cgagcggggg ggcaccttcc ccgatcccga gctcatctag ccccgggatc gggttgccgt
     2101 acggcgacgt ggtcgggttg cccagcagcg ctaccagctt gcgctccacc gcctcgctca
     2161 tcacgtgttc ccagcggcag gcctcgttgt gtacctgctc ccactccagc ccgatcacgt
     2221 ccacgagcag acgctcggct agccggtgct tacgcatcac cgacgtggcc cgattgcggc
     2281 cctctgcggt gagctcgagg tgccggtcgt cggccactgt cagcaggccg tcacgctcca
     2341 tccgcgctac tgtctggctg acggttggcc cgctttgtcc caggcgttcg gcgatccgtg
     2401 cccgcagcgg gacgacacct tcctcctcta gctcgaagat ggtgcgcagg tacatctcgg
     2461 tggtgtcgat gagatcgttc acaccagtct cctttacttg gtgccatgat agtggtccac
     2521 cggggacgct gcggagtatt cgctggcagt gaccgaaaag atgggtgata tgcatccgtt
     2581 gatcacgccc cgtcagctgg cggccctcct gaccgatctg gagccgccca tcctcgtgga
     2641 cgtgcggtgg tcactgaccg gggcacccgg ttgcgccgat taccaggctg ggcacctgcc
     2701 cggggcggtc ttcctcgata tggacaccga gctggctggt tcgccgggtc gggagggacg
     2761 gcatcccctt ccggatccgg cagcgttaca agccaccctg cgcgccgcag gtgtccgcac
     2821 gacagcaccg gtagtcgcct acgacgcggc ggacgggtcg gtggcggcgc ggctgtggtg
     2881 gttactgcgc tgggcggggc actccgcggt cgcggtgctt gacgggggaa ttgcggcgtg
     2941 gcgtgcggct gggctaccgg tgaccaccga ggtacccgaa cccgtcgcga gcactatcac
     3001 ggtgcggccc ccagcgatgc ccgtgctcga cgcggcgcag gccgcggcgt tggcccgcaa
     3061 tggcgtgttg ctcgacgcgc gggtgccgcc ccgctaccgc ggggagatcg agccggtgga
     3121 cccccgggca ggacacgtac ccggcgccgt caatgcgccg ttcgacgagc tgactgatgc
     3181 agacgggcga tggttgacgc cgaggcggct gcggggactc gcgcagcgtt ggggcgtcga
     3241 aagcacagag attggcgcct attgcggttc aggcgtcaac gcgtgcgcac tggttctggg
     3301 actggaactt gccggaatca cctcgcccga gcggccggcg gggctctacg tcgggtcatg
     3361 gtcgcagtgg tgcaccgacc gtgaccgccc agtggccacc ggcccgagtc ccgggtagcg
     3421 gtggtgaccc ggccgcgtcc ccgcaccggg cggtaacgtc ggaggcatga gtgctgctcg
     3481 cgcggctgtc gtctgggacg aggccttcct cggctacgac ctcggtgggt ggcacccact
     3541 gaatcccatc cggctggatc tcaccatccg gctggcccgt tcgctgggtg tcctggacgg
     3601 cgtttcactg ctgacaccgg tgcaggcgac tgacgaacag ctgcgcatgg tgcactccga
     3661 tgactacctt gccgcggtca agaacgcgtc gcgtggatat gtcgggcacg gcctggggac
     3721 ctcggacaat ccggtgtttc gccgcatgca cgaggcggcg gcgctggtcg caggtggctc
     3781 attgctcgcg gccgaggaga tcctcgctgg ccgcgcagcg cgggtggcga atatcgccgg
     3841 cgggctgcac cacgcgatgc gcgactatgc ctcaggtttt tgtatttaca acgactgcgc
     3901 gctggcgatt cgtcggctgc ttgacggtgg ggtgtcccgg gtggcctatg tcgacatcga
     3961 cgtgcatcac ggcgacggcg tgcaagaggc tttcgtcgac gacccccggg tgctcaccat
     4021 ctcactgcac cagcacccgg acacgctctg gccgggcacc ggggactcca ccgacgtcgg
     4081 ggctcctggc gccgaaggtt cggcggtcaa cgtgccgctt ccacccggta ccggcgacgg
     4141 cgaatggttg cgggccttcc acgcggtag
//
LOCUS       DATADN010000707         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1450512, whole genome shotgun sequence.
ACCESSION   DATADN010000707 DATADN010000000
VERSION     DATADN010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450512"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VHH53_09170"
     CDS             <1..658
                     /locus_tag="VHH53_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2290345.1"
                     /translation="DQIRVVVNDIGVAAAKTGMLASAAIISAVAEVCDEVSIGRSVPL
                     VVDPVAASMHGDPLLADDALAALRSQLVPRATLITPNLDEVRLLVGIDVGSRAEQYIA
                     AKQLHALGPRWVLVKGGHLRDDLQDKDPQCLDLLYDGNDFTAFTGPRLDTPHTHGGGD
                     TLAAAITSALARGWTMVEAVGYGKRYIEQAVAHAYPLGAGIGPVSPFWRIPPPWASRA
                     "
     gene            complement(1064..1741)
                     /locus_tag="VHH53_09175"
     CDS             complement(1064..1741)
                     /locus_tag="VHH53_09175"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX2290346.1"
                     /translation="MVAVICDGVSSADRTDEAAGTATEVAAEALVAALHVGDRPAAAM
                     RKAIHAAGQAVAGLAGSTQTTRDAPACTLVSAVVTGGTVTVGWVGDSRAYWIPRTRTS
                     SALPTRLTEDDSWLAQVLAAGTLTLAEAATDQRAHSITAWLGEDATPLDPHVVTFTPE
                     GPGVVVVCTDGLWHYLDNVDALAAALPVQAPDAPLDAARRLVEIALDCGGRDNITAAV
                     LPFGSPR"
     gene            1748..>1950
                     /locus_tag="VHH53_09180"
     CDS             1748..>1950
                     /locus_tag="VHH53_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290347.1"
                     /translation="MLTSPRAGARARPEAAQRQGVLIAVVPPAPIADPGGNPYVDLDA
                     VSRCHWPAIVATRYLRSASTAAFG"
BASE COUNT          298 a          657 c          659 g          336 t
ORIGIN      
        1 cgaccagatc cgggtggtgg tcaacgatat cggcgtcgcc gccgccaaga ccggcatgct
       61 ggcatcggcc gcgatcatct ccgcagtggc cgaggtgtgc gacgaggtga gcatcggtcg
      121 ctcggtgccg ttggtggtcg atccggtagc cgcttccatg cacggcgacc ctttgctggc
      181 cgacgacgcc ctggccgccc tgcggtcgca actggtgccc cgggccaccc tgatcacgcc
      241 gaatctggat gaggtgcggt tgctggtggg catcgatgtc ggttcccggg cagagcaata
      301 catcgcagcg aagcaactac atgccttggg gccgcggtgg gtactggtca agggcggcca
      361 cctgcgtgac gatctccagg ataaggaccc gcagtgcctg gatctgctct acgacggcaa
      421 cgacttcacc gcattcaccg gaccgcggtt ggacaccccg cacacccacg gtggtggtga
      481 caccctcgcc gcggcgatca cttctgcact ggcccgtggt tggacgatgg tcgaggcagt
      541 tgggtacggc aagcgctaca tcgagcaggc ggtggcacac gcatacccgc tcggggcggg
      601 aatcggcccg gtctccccat tctggcggat cccgccgccc tgggccagta gggcatgatc
      661 gcggtttgtg tgcgttgcac gaccatattt gtcgcgcaag gcacacaacc ggtgatcatg
      721 gtggtttacc gcccagctaa ttcccgaggt tcgcctcgtc gttgtcgagc aatctcctgc
      781 ctggcggccc caagctcttt ccattgctcg ttcagggagc gctgtactgc tgcctgccgc
      841 ctatcccgcg catctaacag ccactgactg acagtgcatc ctatgagcac gccaaaactc
      901 accaagaggg ctgccgatag gatgatcaaa gcaatggcgt ccatattccc tccttagata
      961 gaagtagccc ggtgtgagtt tcgtcgctta tcagtgaagc ttttcgccac tatctgtggc
     1021 tagggggcag ccctcaccag cgggtagtca gtcgcagcgt tgctcagcgg ggagagccga
     1081 acgggagtac agcggccgtg atgttgtcgc ggccgccaca gtccagcgcg atctcgacga
     1141 gccggcgcgc cgcgtccagt ggggcgtcgg gtgcctgcac cgggagtgcc gcagcgagcg
     1201 cgtcgacgtt gtcgaggtag tgccacagcc cgtcggtaca gaccacgaca acgcccggtc
     1261 cttcgggtgt gaaggtgacg acgtgtgggt ccagcggggt ggcgtcctcg cccagccatg
     1321 cggtgatgga gtgggcgcgc tggtcggtgg ccgcttcggc gagggtcaga gttccggccg
     1381 ccagtacctg ggccagccac gagtcgtcct cggtcagcct cgtgggcagc gccgagctgg
     1441 tgcgggttcg cggtatccag tacgcccggc tgtctccgac ccagcccacc gtgaccgtcc
     1501 cgccggtgac gacagcggag accaacgtac aggccggggc atctcgggtg gtctgggtgg
     1561 agccggctag tccggcgacc gcctggccgg ccgcatggat ggccttccgc atcgcagcgg
     1621 ccggccggtc gcctacgtgc agcgcggcca ccagcgcctc agcggcgacc tcggtggcgg
     1681 ttccggccgc ctcgtcggta cggtccgccg acgacacccc gtcacagatg accgcgacca
     1741 ccgcgccgtg ctgaccagcc ccagggccgg tgcccgggcc cggcccgagg cggcgcagcg
     1801 ccaaggcgtc ctcattgcgg tggtgccgcc ggccccgatc gctgaccccg gcggcaaccc
     1861 ctacgtcgac ctcgacgcgg tctcgcggtg tcactggcct gcgatcgtgg cgacgaggta
     1921 cctgcgcagc gcctcgaccg ccgccttcgg
//
LOCUS       DATADN010000708         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_691412, whole genome shotgun sequence.
ACCESSION   DATADN010000708 DATADN010000000
VERSION     DATADN010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691412"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VHH53_09185"
     CDS             <1..596
                     /locus_tag="VHH53_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX2290348.1"
                     /translation="GWAAGSATLFTGTSWVVGTDDGSDPTVWTQVARLALDCGATVVP
                     AGTEDHDAAVARASHLPHVLAAVLASVGADGGPLAMALGAGSFTDGTRVAASPPELVR
                     AMCEGNRSALLEAVDDALGRLGAARGSLASTGALDATIRAGHLARRAFDAARDGRDGD
                     TVHVDLAAPGALARLGEIARSGGRVIALHGARCQVAL"
     gene            670..1893
                     /locus_tag="VHH53_09190"
     CDS             670..1893
                     /locus_tag="VHH53_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX2290349.1"
                     /translation="MWRLEAMVAALSDDPYNAIVQRVAVQPALPGPLSGWPVAVKDLI
                     DVAGLPTRCGSAVLADAVPAQQDAAVVARLRAAGAVVVAKTHTHEFAYGPTGDVSVDG
                     PCHNPHDPQRITGGSSSGSAAAVAAGLVPLALGTDTGCSTRTPAALCGVVGLKPARGA
                     LPTAGVFPLSETLDHIGLLGAGIDTVAVAFSALTGHRIDLAVPVEGTVVGRPTDSYWR
                     VHDPEITAAVDRAAAALETAGAILRDVRLPAIDELAATYYVIVDAEAYATHARWLVQR
                     PGDYQPITAARLQAAARHTAADYVTAQRTRRRLATELTDRLHTAAVDLLLLPTTPLRA
                     TPIGAEQVHAPDGTQVAVRPALLSLTLPFNLLGWPAVSVPAPEVAGLPAGIQVAGVQG
                     GEQAVLAAGACIAVR"
     gene            complement(1901..>2347)
                     /locus_tag="VHH53_09195"
     CDS             complement(1901..>2347)
                     /locus_tag="VHH53_09195"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEX2290350.1"
                     /translation="GVVQRLTVRTIRAQGEQYISLTRSEEGIWLVDRVDHGVGTVRTQ
                     FAGALDVDLAFCTLFKALPVRRLGLHRPPAQPDAPQQDLTQYDDLPVAFVSLPGLEVS
                     CVRQTYSTVAVGETTVVSVANDLFKVELTVDADGLVLEYPGLARRT"
BASE COUNT          338 a          841 c          823 g          345 t
ORIGIN      
        1 cgggttgggc ggccggctcg gcgacgttgt tcaccgggac gtcctgggtg gtgggcaccg
       61 acgacggtag tgaccccacg gtgtggacgc aggtcgcccg gcttgcgctg gactgcggcg
      121 ccaccgtggt accggccggc accgaggacc acgatgccgc ggtcgcgcga gcatcacacc
      181 tgccgcacgt gctggccgcg gtactcgcct cggtgggggc cgatggcgga cctctggcaa
      241 tggcactggg cgcgggctcg ttcaccgacg gcacccgggt ggccgcttcc ccacccgaac
      301 tggtgcgcgc gatgtgcgag ggcaaccgca gcgcactgct ggaagcggtg gacgatgcgc
      361 tcggccggtt aggcgcagcc cgcggctcac tggcgtccac tggcgcgctg gacgccacca
      421 tccgggccgg ccaccttgcc cgccgagcct tcgacgcagc ccgcgacggt cgcgacggtg
      481 acaccgtgca cgtcgacctg gccgcgcccg gcgcactcgc gaggctaggc gagatcgccc
      541 gatctggcgg tcgcgtcatc gccctgcacg gcgcccgctg ccaagtcgcc ctctaatcaa
      601 ccacaccgcc ggactgcgat atggatgctt gtcgcagtga aagggcttcg tcgagtgtga
      661 ccagtatcca tgtggcggct ggaggcgatg gtggcggcgc tgagcgatga tccgtataac
      721 gcgatcgtgc agcgggttgc ggtgcagccg gcgctgcccg ggccgttgag cggctggccg
      781 gtggcggtga aggacctcat cgacgtggcc gggctaccca cccgctgtgg tagcgcggtc
      841 ctggccgacg ccgtcccggc gcagcaagac gccgccgtgg tggcccggtt acgcgcggcc
      901 ggcgcggtgg tggtcgccaa gacccacacc cacgagttcg cctacggacc caccggggac
      961 gtctccgtgg acggcccgtg ccacaatccg cacgatccgc agcggatcac cggtggctcg
     1021 tcgagtggat cggcggccgc ggtagccgcg ggactggtgc cgctggcgtt aggcaccgac
     1081 accggctgct cgacgcgtac cccggccgcg ctgtgcggcg tggtcgggct caaaccggcg
     1141 cgcggtgcgc tgcccaccgc tggggtgttc ccgctgtcgg agaccttgga ccacatcgga
     1201 ctgctgggcg ccggcatcga cacggtcgcg gtcgccttca gcgcgctgac cgggcaccgg
     1261 atcgacctgg cagtcccggt cgaaggcacc gtggtcgggc gccccaccga ctcctactgg
     1321 cgggtgcatg atccggagat caccgccgcc gtggatcgcg ctgccgccgc gctggaaact
     1381 gcgggggcta tcctgcgcga cgtcaggctg cccgcaatcg atgaacttgc cgccacctac
     1441 tacgtgatcg tcgacgcgga ggcctacgcc acgcacgcgc gctggctggt ccagcgcccc
     1501 ggcgactacc agccgatcac cgcggcacgg ctgcaggccg ccgcccggca caccgccgcc
     1561 gattacgtca cggcccagcg gacccgccgc aggttggcca ccgagctgac ggatcgactg
     1621 cacaccgcag ccgtcgatct gctgctgctg ccaaccacgc cgctgcgcgc cactccgatc
     1681 ggcgccgaac aggtccacgc accggatggc actcaggtcg cggtgcggcc ggcgctactg
     1741 tctctcaccc tgcccttcaa cctgctgggc tggcctgcgg tgtcggtgcc cgcgccggag
     1801 gtggccgggc taccagccgg gatccaggtt gccggggtgc agggcgggga gcaggcggta
     1861 ctcgccgccg gtgcttgcat cgcggtccgg tgacgccgac tcaggtacgc cgggctaatc
     1921 cggggtattc caacaccagg ccgtcggcgt ccaccgtgag ctccactttg aacaagtcgt
     1981 tggcgacgct caccactgtg gtttcgccca ccgccacggt gctataggtc tgccggacgc
     2041 aactcacctc gagtcctggc aacgagacga acgccaccgg caagtcgtcg tactgggtca
     2101 aatcctgctg gggcgcgtcg ggctgggcgg ggggacgatg caaacccagc cggcgcacag
     2161 gcaaagcctt gaacagcgtg cagaacgcca gatcgacgtc cagcgctccg gcgaactggg
     2221 tgcgcacggt gcccacgccg tggtcgaccc ggtcgaccag ccagatgccc tcctccgagc
     2281 gagtaaggct gatgtactgc tcgccctgcg cccgaatggt gcgcacggtc aaccgctgta
     2341 ctacacc
//
LOCUS       DATADN010000709         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1151406, whole genome shotgun sequence.
ACCESSION   DATADN010000709 DATADN010000000
VERSION     DATADN010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151406"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1754
                     /gene="carB"
                     /locus_tag="VHH53_09200"
     CDS             <1..>1754
                     /gene="carB"
                     /locus_tag="VHH53_09200"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2290351.1"
                     /translation="AQASGIDPWFVDQIAGLVELRAELVAAAVLDQELLRRAKRAGIS
                     DRQLAAVRSDLTEPEVTALRHREGVRPVYKTVDTCAAEFAARTPYHYSAYETDPDAET
                     EVTSQRERPKVIILGSGPNRIGQGIEFDYSCVHAAMALGSCPNPPGGYETVMVNCNPE
                     TVSTDYDTADRLYFEPLTFEDVMEVIEAERTSGTVAGVIVQLGGQTPLGLAQRLADAG
                     VPVVGTTPEAIHLAEDRGSFGAVLERAGLPAPAYGTATSFAEARKIAEEIGYPVLVRP
                     SYVLGGRGMEIVYDEETLAGYITRATEITPEHPVLVDRFLDDAIEIDVDALCDGSEIY
                     LGGIMEHIEEAGVHSGDSSCVLPPITLGESDLVTVRASTEAIARGIGVRGLLNVQYAL
                     KGDVLYVLEANPRASRTAPFVSKATAVPLAKAAARIMLGASIAQLRTEGLLLAHGDGG
                     DLPADAPVAVKEAVLPFHRFRTPEGQGVDSLLGPEMKSTGEVMGIDAAFGQAFAKSQA
                     AAYGSLPTSGRVFVSVANRDKRAMVFPAKRLADLGFEVLATRGTANTLRRNGIPCTVV
                     RKHFEGPDNIVELINAGE"
BASE COUNT          286 a          589 c          591 g          288 t
ORIGIN      
        1 ggcgcaggcg tcgggtatcg acccgtggtt cgtcgaccag atcgccggcc tggtcgagct
       61 gcgcgccgaa ctcgtcgcgg cggcggtact ggaccaggag ttgctgcgcc gcgccaagcg
      121 tgccggtatc tctgaccggc agctcgccgc cgtgcgcagt gacctgactg aacccgaggt
      181 caccgcattg cggcatcgcg agggcgtacg gccggtctac aagaccgtgg atacctgcgc
      241 cgccgagttc gccgcccgca cgccctacca ctactccgcc tacgagaccg atcccgatgc
      301 cgagaccgag gtcacctcgc agcgggaacg acccaaggtg atcattcttg gctccgggcc
      361 gaaccggatc gggcagggta tcgaattcga ctattcctgc gtgcacgctg cgatggcgct
      421 gggttcgtgt cccaacccac ccggcggcta tgagaccgta atggtcaact gcaacccgga
      481 aaccgtgtcc accgactatg acaccgccga tcggctctac tttgagccgc tgaccttcga
      541 agatgtcatg gaggtgatcg aggccgagcg gacatcgggc accgtggcgg gtgtgatcgt
      601 gcaactgggc gggcagaccc cactcggcct ggcacagcgg ctggccgacg ctggcgtccc
      661 ggtggtgggc acgactcccg aggcgattca cctcgcagag gaccgcggct ccttcggcgc
      721 ggtgctggaa cgggccgggc tacccgcacc cgcgtacggt accgccacct cgttcgcgga
      781 ggcccgcaag atcgccgagg agatcgggta cccggtcctg gtccgtccct cttacgtgct
      841 cggtgggcgc gggatggaga tcgtctacga cgaggagacg ctggccggtt acatcacccg
      901 tgccaccgag atcacgcccg agcacccggt gctggtggac cgattcctcg acgacgccat
      961 cgagatcgac gtcgacgcgc tgtgcgacgg cagtgagatt tacctcggcg ggatcatgga
     1021 acacatcgag gaagccgggg tgcactccgg ggactcctcc tgcgtgttgc cgccgatcac
     1081 gctgggcgag tctgacctgg tcacagtgcg tgcctcgacc gaggcgattg cccgcggaat
     1141 cggcgtccgc ggtctgctca acgtgcagta cgccctcaag ggcgacgtgc tctacgtgct
     1201 ggaggccaac ccacgggcca gccgcacggc gccgttcgtc tccaaggcca ccgcagtgcc
     1261 actggccaaa gccgctgctc ggatcatgct cggtgccagc atcgcgcagc tgcgaactga
     1321 gggcctgttg cttgcgcacg gggacggggg tgacctgccg gccgatgcac cggtggcggt
     1381 caaggaggcc gtgctgccct tccatcggtt ccgcaccccg gagggtcaag gggtggactc
     1441 gctgctgggg ccggaaatga agtccaccgg cgaggtgatg ggcatcgatg ccgcattcgg
     1501 tcaagccttc gcgaagtccc aggccgcggc ctacggctcg ttgccgactt cgggccgggt
     1561 gttcgtgtcg gtggccaacc gggacaagcg ggccatggtg ttccccgcca aacggctggc
     1621 cgatttgggc ttcgaggtcc tcgccacccg cggcaccgcc aacacgctgc gccgtaacgg
     1681 gatcccttgc accgtcgtgc gcaagcactt cgaaggtccc gacaacatcg tcgagttgat
     1741 caacgccggt gaga
//
LOCUS       DATADN010000710         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_229340, whole genome shotgun sequence.
ACCESSION   DATADN010000710 DATADN010000000
VERSION     DATADN010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229340"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VHH53_09205"
     CDS             <1..223
                     /locus_tag="VHH53_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290352.1"
                     /translation="PGVTVHQPAAAPFLLLRVPHGEQVRACLADAGIAVRRADTFPGL
                     TPDHLRVAVRPPEIATLLVTALRTALCAS"
     gene            303..1151
                     /locus_tag="VHH53_09210"
     CDS             303..1151
                     /locus_tag="VHH53_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX2290353.1"
                     /translation="MTATVQDVVAVLDAAYPPGLAEGWDAVGLVCGDPAEPVQRVLVA
                     VDPVAETVAEALEQDVQLLVTHHPLLLRGVHGVPADDPKGALVHRLVRAGVGLFCAHT
                     NADAANPGVSDALAHALGLRVTGVLDVREGFSRTGLGRTCELAQPESLQAFTSRVVGA
                     LPETAWGVRAAGDPQRPIRTVAVCGGAGDSLLGAAASARVDAFVTADLRHHPASEHLA
                     ADGPALVDVAHWASEHPWCPQAAAVISTALGGTVDVHVSYRRTDPWTIGLTRSDRAQS
                     DGRLPR"
     gene            1148..>1527
                     /locus_tag="VHH53_09215"
     CDS             1148..>1527
                     /locus_tag="VHH53_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290354.1"
                     /translation="MNADPAAQRRLLELAVVDAELTRAAHRRRSLPELAEIEQVDKDL
                     LSGRDSLVAAETAASDLDREIRKLEAEIDQVRARENRDRGLLDSGSISSGKQLEDLQH
                     ELATLQRRQTVLEDDLLEVMEQREA"
BASE COUNT          225 a          522 c          534 g          246 t
ORIGIN      
        1 gcccggtgtc acggtccacc agccggctgc ggcgcccttc ctactgttgc gcgtgccaca
       61 cggcgagcag gtccgagcct gccttgccga cgccggtatc gcagtacggc gcgccgacac
      121 cttccccggg ctcacccccg accacctgcg cgtcgcggtc cgcccccccg agatcgccac
      181 cctgctcgtc accgcacttc gtaccgcgct gtgtgcatca tgacctctga cgccattcgg
      241 agccgaatag cggttgtcca ggaccgccga ggcgccattc ggagccggat cgcggttcgc
      301 ggatgaccgc gaccgtgcag gatgtggttg ctgtgctgga tgctgcatac ccgcctggac
      361 tggctgaggg ttgggatgcg gtggggctgg tctgcggtga tccggccgaa ccggtgcagc
      421 gggtgctggt ggccgtcgat ccggtcgccg agaccgtcgc cgaggcgctg gagcaggacg
      481 tgcaactgct cgtcacccac catccactgc tgctgcgcgg agtgcacggc gtgcccgccg
      541 acgatcccaa aggcgccctg gtgcaccggc tggtgcgcgc tggggtcggg ttgttctgcg
      601 cgcacaccaa cgccgatgca gccaacccag gagtatccga tgcgttggct cacgcgctgg
      661 ggttgcgggt caccggcgtg ctcgacgttc gtgaagggtt ctcgcgcacc gggctcgggc
      721 gaacctgcga gctggcccaa ccagaatcgt tgcaggcgtt caccagccgg gtggtggggg
      781 cgctgcccga gaccgcgtgg ggtgtacggg ccgccggtga cccgcaacga ccgatccgta
      841 ctgttgcggt ctgcggcggt gccggggact cgctgctcgg tgccgccgcc agcgcacgcg
      901 tcgatgcctt cgtcaccgct gacctgcggc atcacccagc gtctgagcac ctggccgccg
      961 acggtcccgc gctggtcgac gtcgcacact gggccagtga acacccgtgg tgcccgcagg
     1021 ctgcagcggt gatctccacg gcgctcggcg gtacggtcga tgtccacgtt tcgtaccgcc
     1081 gcaccgatcc ctggacaatc ggcttgaccc gatcagatag ggcacaatca gatgggaggc
     1141 tgccgcggtg aacgccgatc ccgccgcgca acgccggctg ctcgagctcg ccgtggtgga
     1201 cgccgaactc acccgtgccg cgcaccggcg caggtcgctg cccgagctgg ccgagatcga
     1261 gcaagtcgac aaggacctgc tgtcgggccg ggactcgctg gtcgcggcag agacggcggc
     1321 atcggacctg gaccgtgaga tccgcaagtt ggaggccgag atcgatcagg tgcgagcgcg
     1381 cgagaatcgc gaccgcggtc tgcttgactc cggatcgatc agttccggca agcagctgga
     1441 ggacctgcag catgagttgg ccaccttgca acgccgccag actgtcttgg aggacgacct
     1501 gctggaggtg atggagcaac gcgaggc
//
LOCUS       DATADN010000711         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_398337, whole genome shotgun sequence.
ACCESSION   DATADN010000711 DATADN010000000
VERSION     DATADN010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398337"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VHH53_09220"
     CDS             <1..393
                     /locus_tag="VHH53_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290355.1"
                     /translation="RSFCLKEFKSGATRMARAAGVPLIPVALWGSQRVWTKDRKPTIR
                     AARHIPIGITIGPPVRPGRGTAPDIELVDAINDLLDVTRARYPEAPGNDPWWVPAHLG
                     GTAPTPEEAAALDAEEQLERARRRDARG"
     gene            complement(394..1332)
                     /locus_tag="VHH53_09225"
     CDS             complement(394..1332)
                     /locus_tag="VHH53_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX2290356.1"
                     /translation="MTTTSDSVRLLHDLEPVVTENLNRHLAAAKEWMPHEYVPWSLGR
                     DYADLGGQPWHPEQSRLSPIAQTALEVNLLTEDNLPSYHREIERAFGRDSAWGTWVHR
                     WTAEEGRHAYCIRDYLLVTRGVDPVELERARMQVMQTGYNTQDAPLLRVLAYVSFQEL
                     ATRISHRNTGRYAEDPIAERLLTRVSTDENLHMVFYRNLVKAALEVSPGPTLRAITDE
                     VTNFHMPGIVIPGFARKAALIAKAGIYDLRIHHDEVIWPLLRQWGVFELDSVDAEGEK
                     ACAELADFLSQLDGQASRFEERRAQAQARARAATQQ"
     gene            1458..2096
                     /locus_tag="VHH53_09230"
     CDS             1458..2096
                     /locus_tag="VHH53_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HEX2290357.1"
                     /translation="MPRPDAPQPLAALLGGGRAALDATTPAVAFVVAWLVSGRSLGWG
                     AGAALVTGAAVAAARWCRGDRPLAALLGVLGVSVAALIALYTGRAADFFLAQLLSNVV
                     SALVWAVSVLVRWPLLGVIVGGVLGQRTSWRQDPALLRAYCLASWVWVGQYLIRVAVF
                     TPLWVTGAVVALGAARVLLSWPLVAASVAVSGAVLIRVLPANHPGLRSVRRS"
BASE COUNT          297 a          710 c          838 g          420 t
ORIGIN      
        1 cgcagtttct gcctgaagga gttcaagtcc ggcgccacgc ggatggcgcg tgccgctggc
       61 gttccactga tcccggtggc gctgtggggc agccagcggg tgtggaccaa ggatcgcaag
      121 cccaccattc gcgccgcgcg gcatatcccg attggcatca ccatcggccc acccgtgcgg
      181 ccagggcgcg ggaccgcgcc cgacatcgag ctggtcgacg cgatcaacga cttgctcgac
      241 gtcacccggg cccgctatcc cgaagccccc ggcaacgacc cctggtgggt gccggcacat
      301 ctcgggggaa ctgccccgac accggaagag gccgcagcgc tggacgccga ggagcagttg
      361 gagcgcgccc ggcgccggga cgcccgaggg tgactactgc tgggttgccg cccgcgcccg
      421 cgcctgcgcc tgcgcgcggc gctcctcgaa tcggctggcc tgcccatcga gctggctgag
      481 aaagtcggcg agttcagcgc acgccttctc gccctccgcg tcgacgctgt ccagctcgaa
      541 cactccccat tggcgcagca gtggccagat cacctcgtcg tggtggatgc gcagatcgta
      601 gatgccggct ttggcgatca gcgcggcctt gcgggcgaat ccggggatga cgatgcctgg
      661 catatggaag ttggtgacct cgtcggtgat cgcgcgcagg gtcgggccgg gggagacctc
      721 cagggcagct ttgacgaggt tgcggtagaa gaccatgtgg aggttctcgt cggtggacac
      781 tctggtgagt agtcgttcgg cgatggggtc ttcggcgtag cggccggtgt tgcgatgcga
      841 gatgcgagtg gcgagctctt ggaaggacac gtaggcgagg acgcgcagca gcggtgcgtc
      901 ttgggtgttg tagccggttt gcatgacctg catccgggcc cgttccagct ccaccgggtc
      961 gaccccgcga gtcaccagca ggtagtcgcg gatgcagtac gcgtgccggc cctcctcggc
     1021 ggtccagcga tgcacccagg tgccccaggc gctgtcacgt ccgaatgcgc gctcaatctc
     1081 gcggtgataa ctgggcagat tgtcctcggt gaggaggttg acctccagcg ccgtctgggc
     1141 gatcggcgac agccgcgact gctccggatg ccagggctgc ccgccgaggt cggcgtagtc
     1201 gcggcccagg ctccacggca cgtattcgtg cggcatccac tccttggcag cggccagatg
     1261 ccggttgagg ttctcggtga ccaccggctc gaggtcgtgg agtaaccgga cgctgtccga
     1321 ggtcgtcgtc atggcggtcc tcagctgctg gtcgagacgg gcgttctgtg gcaaccgtag
     1381 cgtttaggat gcgcgcaccg gtgacgcgtg tgatcccgcc ttcggaggaa ccggcggtaa
     1441 tcaggcaccc tggacgggtg ccgcgtccgg atgcgcccca gccgttggcg gcgctgctgg
     1501 gtggtggccg agcggcgctg gatgccacca cgcccgccgt cgccttcgtc gtggcctggt
     1561 tggtctcggg tcgctccctg gggtgggggg cgggggcggc actggtcact ggggccgccg
     1621 tagcagcggc gcgctggtgc cgcggtgacc gcccgctggc cgccctgctg ggcgtactgg
     1681 gcgtctcggt ggcggcgctg atcgcgttgt acaccggtcg tgccgctgac ttctttctcg
     1741 cccagttgct gtccaacgtc gtcagcgcac tggtctgggc ggtcagtgtg ctggtccgct
     1801 ggccgctgct cggggtgata gtgggcgggg tgctgggcca gcgcacatcc tggcggcaag
     1861 atccggccct gctgcgggcg tactgcctgg cgtcctgggt gtgggtgggg cagtacctga
     1921 tccgggttgc ggtgttcacg ccgctgtggg tgaccggcgc cgtggtggcg ctcggagccg
     1981 ctcgcgtgct actgagctgg ccgcttgttg cggccagcgt cgcggtgagc ggggcggtgc
     2041 tgattcgggt gctgcccgcg aatcaccctg ggctgcgctc ggtacggcgg tcctgatctg
     2101 gcaccgttct cgctacgcgt agtgagaaga acactggccg tatgagtgac caagacccgc
     2161 cgaatgggct cttcaacagt gtgtaactga tccgtagcgt ggggtgactg tcggggagtc
     2221 atgtgctctg tgtgcttttt aaggaaaggg ccgatccgtg cttcc
//
LOCUS       DATADN010000712         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_72926, whole genome shotgun sequence.
ACCESSION   DATADN010000712 DATADN010000000
VERSION     DATADN010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72926"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VHH53_09235"
     CDS             complement(<1..1277)
                     /locus_tag="VHH53_09235"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEX2290358.1"
                     /translation="MEPRCPGCDVPARRIHSRYIRTLADLPWSGSRVTWRLRVRKFFC
                     GHPPCPCRIFTERLPGLAAPWARRTLRLATRLLAIGLALGGAAGARLSQSFGLPVSRN
                     TLLRVIRRAPCPAISSPQVLSVDDFALRRRHTYGTILLDLERRRPLALLPDREATTVA
                     QWLEAQPGIEVVVRDRAEAYAEAARRGAPTACQVADRFHLLQNLADTLTQVFTAHASQ
                     LARVHPPRAAPTPLHDPAAPATGSSPSSVPLAPPPSSTAAARLARQRRTRRWAQYHQV
                     WTYHQQGWTLDAIAQQVGRSRRTVQRYLQSPTFPERSPRHTRDRSLLDPYKATLLAGW
                     NNGCRNGWHLFRTIRRQGFRGQYGIVALYVRRMHQAQGLAPWQRRSPRPLPTVTEGSR
                     RPLTPCRATWLVLRPSERLTAPDHHQLAQLTQQA"
BASE COUNT          271 a          501 c          603 g          239 t
ORIGIN      
        1 gcctgctggg tcagctgtgc cagctggtgg tgatcgggcg ctgtcaaccg ctccgatgga
       61 cggaggacca gccaggtggc tcgacacggg gtgagcggcc gtcgtgaccc ctccgtcacc
      121 gtgggcagcg ggcgagggct gcgccgttgc cagggcgcca acccttgggc ctggtgcatg
      181 cgccggacat acagggcgac gatgccgtac tgaccccgaa acccctggcg gcggatggtc
      241 cggaagagat gccagccatt gcgacagccg ttgttccagc cggccaggag ggtggctttg
      301 tacgggtcga ggaggctgcg gtcgcgggta tgccgcggtg agcgctcggg aaaggtcggg
      361 ctctgcagat agcgctgcac ggtgcgccga ctgcgcccca cctgctgcgc gatggcgtcc
      421 agcgtccacc cctgctgatg gtacgtccag acctggtgat actgggccca ccgtcgggtc
      481 cgtcgttggc gggccaaccg tgccgcggcg gtggacgagg ggggcggagc gagcggcacc
      541 gaggaggggc tggagccggt ggcaggggcg gcggggtcgt gcagcggcgt gggcgcggcg
      601 cgtgggggat ggacccgcgc cagctgggag gcatgggcgg tgaacacctg ggtcaacgtg
      661 tcggccagat tctgcagcag atgaaagcgg tcggccactt ggcaggcggt gggcgccccg
      721 cggcgcgccg cttcggcgta ggcctccgcg cggtcccgca cgaccacctc gatcccgggc
      781 tgggcctcaa gccactgcgc cacggtcgtg gcttcccggt cggggagcag cgccaggggc
      841 cgccgccgtt ccaggtccaa gaggatagtg ccgtaggtat gacgcctccg cagcgcgaaa
      901 tcgtccacgc tgagcacctg tggggaactg attgccgggc agggagcgcg acggatcacc
      961 cgcaacaggg tgttccgact gacggggaga ccgaacgatt ggctcagccg cgctccagcc
     1021 gcccccccga gggccagccc aatggcgagg aggcgtgtcg cgagccgcaa ggtccgccgg
     1081 gcccaaggcg ccgcgaggcc gggtagtcgt tccgtaaaga tgcggcatgg gcagggcggg
     1141 tggccacaga agaacttccg gacgcggagc cgccatgtca cccgggaccc gctccacggg
     1201 agatcggcca gcgttcggat gtaccgacta tggatgcgcc gggctgggac atcacaccct
     1261 gggcagcggg gttccacttg tgtggacgag accaagaggg tgatctgcgc gcgtgcttcg
     1321 tcgaggtgcc aagcatccag gtggagcggg atggcgttag gcaagacatg ggccagcgtg
     1381 tctcagacct ccatagatgc tcaagcgttg gttctttgag aaaatccccc tctcaatata
     1441 cccctccaac agccacggga aagacctcac caaaaccggc caagagcccg atttaacttg
     1501 caaaacgcct ctcgccgtga ggacatcagg cgtcgcggct cagcggatca cctcggtcgc
     1561 ctggaagagg agcgttggcg gaatggtgat accgagcgcc tgagcggtct tgag
//
LOCUS       DATADN010000713         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1282432, whole genome shotgun sequence.
ACCESSION   DATADN010000713 DATADN010000000
VERSION     DATADN010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282432"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VHH53_09240"
     CDS             <1..367
                     /locus_tag="VHH53_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290359.1"
                     /translation="RGDARQALEIFRDAVRHWHRAGNWTQQWTTVRNVVDLLVRLGAD
                     EPAALLYGAVSTRSTAAPVFGGDADRLDQAHRVLTERLGAQRFTAAAANGMVLGDDDV
                     VGLVCDVIDDPALLAEAGF"
     gene            complement(364..606)
                     /locus_tag="VHH53_09245"
     CDS             complement(364..606)
                     /locus_tag="VHH53_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290360.1"
                     /translation="MTYFTVVEPVNQRLRPLPPGVVPQDFAALRAQWEFGHATEFVLF
                     LVAFVCLVVSVLEHPAGGGHGRGFQAAIASVPRRLR"
     gene            complement(603..818)
                     /locus_tag="VHH53_09250"
     CDS             complement(603..818)
                     /locus_tag="VHH53_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290361.1"
                     /translation="MTSPVGPRDQQMQTGCDTVDVGTWEVFGECFVDRVAAAVSDSGE
                     PQVPVEGTAGDGFRTAQLPQPCRQRPW"
     gene            974..1231
                     /locus_tag="VHH53_09255"
     CDS             974..1231
                     /locus_tag="VHH53_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290362.1"
                     /translation="MLDSDAQVWVNAINELMTTHSPHPVDITNLAQAEGKIGERQVTE
                     TGPGQITVADGWTAVYEPEQQRWVLAPDGDANDSTLPLDPL"
     gene            1251..>1844
                     /locus_tag="VHH53_09260"
     CDS             1251..>1844
                     /locus_tag="VHH53_09260"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX2290363.1"
                     /translation="MTSRYVDLSHPIKHEMTTYPGLPGPTISTHTSRQESAQRLATGV
                     SLHIGRIEMVANTGTYVDAPYHFHADGADVAELPLERLVDVPVVVVRASGRAALGPEV
                     LDDVSALWGKAVLVHTGWSRHWGTPAYLAGSPYLTKGFAEAVVEANVALLGVDALNVD
                     NVDDPTRPVHHTVIGQAVQVGQMLDQRDAVTEHGVVHR"
BASE COUNT          334 a          617 c          619 g          274 t
ORIGIN      
        1 ccgtggcgac gcacggcagg cactggagat cttccgcgac gccgtccggc actggcatcg
       61 ggcagggaac tggactcagc aatggaccac agtccgcaac gtggtcgacc tgctcgtgcg
      121 cctgggagcc gatgagcccg cagccctgct gtacggcgcg gtgagcacgc gcagcacggc
      181 cgcgccggtc ttcggcggcg acgcagaccg gttggaccag gcccatcgcg tcctcaccga
      241 gcgtctcggt gcgcagcggt tcaccgcggc agcggcgaac ggcatggtgc tcggggacga
      301 cgacgtggtg gggttggtgt gtgacgtcat cgacgatccc gcgttgctgg ccgaggcggg
      361 gttttagcgc agacgccgcg gcaccgacgc tatcgccgct tgaaagccgc ggccgtgccc
      421 gccaccggcc ggatgctcca gcacggacac caccaggcac acgaacgcca ccaagaacag
      481 cacgaattcg gtcgcatggc caaactccca ctgggcgcgc agtgcagcga agtcctgggg
      541 cacgacgccg gggggcaacg gtcgcagccg ctggttcacc ggctcgacta ccgtgaagta
      601 ggtcaccagg gacgctgcct gcagggttgc gggagttggg ccgtgcggaa accatcacca
      661 gcggtgccct cgaccggcac ctgcggctca cccgagtcgg ataccgccgc cgcaacgcgg
      721 tccacgaagc actcgccgaa cacctcccag gtgcccacgt ccacggtgtc gcagccggtc
      781 tgcatttgct ggtctcgggg accgaccgga ctcgtcattg aatacgccgc atgcccggcc
      841 gaccagctcc gctaggccat ccggcggctc gggaaccttg gtagcaggcg ccacatgcgc
      901 cgttgatggg aagatggaga gcgcagcggt acagagactg acgccaacga tctcttaacg
      961 aaagtgacca aacatgctgg attccgatgc ccaggtgtgg gtgaacgcca tcaacgagct
     1021 gatgaccacc cactccccac accccgtcga catcaccaac ctggcccaag ccgagggcaa
     1081 gatcggtgag cggcaggtga ccgagacggg acccggccag atcaccgttg cggacggatg
     1141 gacagcggtc tacgagcccg aacaacagcg atgggttctt gcgcccgacg gtgatgccaa
     1201 cgactccacc ttgcccctcg acccgctata aggcctgcgc cgctcgccgc atgacttcgc
     1261 gatacgtcga tctgagccac ccgatcaagc acgagatgac cacctatccc gggctgcccg
     1321 gaccgacgat ctcaacgcat acgtctcggc aggaatccgc gcagcggttg gcgaccgggg
     1381 tgtcgttgca catcggccgg atcgagatgg tcgccaacac cggcacgtac gtcgacgcgc
     1441 cgtatcactt ccacgccgac ggtgccgacg tggcggagct gccgctggag cgattggtgg
     1501 acgtgccggt cgtcgtagtc cgagccagcg gtcgtgccgc gctcggcccg gaagtgctgg
     1561 acgatgtgag tgcgctgtgg ggcaaggcag tactcgtgca cacgggctgg tcgcggcact
     1621 gggggactcc cgcctacctg gccggaagtc cttacctgac aaagggtttc gccgaggctg
     1681 tggtcgaggc gaacgtcgcg ttgctcggcg tcgacgcgct gaacgtcgac aacgtcgatg
     1741 acccgacccg gccggtgcac cacaccgtga tcgggcaggc ggtccaggtt ggtcagatgc
     1801 tcgatcagcg ggatgccgtt accgagcacg gtgtggtgca ccgg
//
LOCUS       DATADN010000714         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_398453, whole genome shotgun sequence.
ACCESSION   DATADN010000714 DATADN010000000
VERSION     DATADN010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398453"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..288)
                     /gene="rpmB"
                     /locus_tag="VHH53_09265"
     CDS             complement(97..288)
                     /gene="rpmB"
                     /locus_tag="VHH53_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX2290364.1"
                     /translation="MAAVCDVCGKGPGFGMSVSHSHRRTNRRWNPNIQTVRAKIGASA
                     RRRLNVCTSCLKAGKVTRS"
     gene            444..2117
                     /locus_tag="VHH53_09270"
     CDS             444..2117
                     /locus_tag="VHH53_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEX2290365.1"
                     /translation="MLQSLDGVAIRHWAATCCDALAAHRDEIDALNVFPVADFDTGSN
                     LLATMRAGLDAVLRDHGDDRSPPSSTVAVLARGALMGARGNSGVILSQILRGLAEAPV
                     GAALDGAALREGLRRAGELATAAVSNPVSGTVLTVLHDAAQAAMGVPSDDLGDVAAAA
                     TIAAAGALAETPRQLDVLAAAGVVDAGGRGLVVLLEALLTVVTECRGMGLTVTAEIPA
                     RPASTRQAPHEVGSSAYEYEVMYLLDGTDEARAGTLRAELAVLGDCVAVVSSGPGAAA
                     LWKVHVHCCDVGAAIEAGIRAGRPHRITVLRLTDRSCADQPGADQSDVVQPGADQSGA
                     TTAQGRFITGHAVLAMVSGSGLAELFRSEGAFTASSTSSATELLAVLAGTRARHITVL
                     ASPAAASRIAEITVAHARDTGQVVVVVPTASAVQALAAIAVHDAGRPAAEDLMAMTEA
                     AAGTRRGELAVASAAAPTWVGCQPGDVLGTVGDEVVLTDCDSLAAARGLAEVMLSEGG
                     ELVTVLLGQHAPDGVGDMLIKHLRAVHPEVETVVYAADVPDSMLLIGVE"
     gene            2121..>3888
                     /locus_tag="VHH53_09275"
     CDS             2121..>3888
                     /locus_tag="VHH53_09275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX2290366.1"
                     /translation="MAAMDDALDRVIGGKPATLLAEHLDVHTIGELLRHYPRRYADQG
                     QLTPIAGLTPGEDVTVVAEVRSCSSRQMQTKKGTIQNAVIWDGSRTLNLTFFNQWRLD
                     KTLTPGRKALFAGRVTTYGKKRTLQLTHPRYQLLDGDADELAEFSRPLIPVYPATKDL
                     PSWTIQLCVRQVFDVLDEPLDPLPQSLRKQHGLLGLGAALRAVHRSDDRSEIAAAHRR
                     LKWDEAMAVQLTLAQRRQEAALRPAPACPRRDGGLAAAFDAALPFTLTAGQRQVGELL
                     AAELASEHPMNRLLQGEVGSGKTVVALRAMLQVVDAGRQAVLLAPTEVLAGQHARSLR
                     DMLGDLGRAGELGSADVATRIVLLTGSLPARARKQALMDAASGAAGIVVGTHALIQDR
                     VSFADLGMVVVDEQHRFGVEQRDALRGRAADGTSPHVLVMTATPIPRTVAMTVYGDLE
                     VCSLRELPGGRSPISTTVVPVGEKPSWLERVWRRITEEIAAGRQAYVVCPRIGGEIGG
                     EDAAGEDTSDGDGSGRPPIAVLDLVPQLTEGPLAGLRLGVLHGRLPGEEKDAVMRSFT
                     VGEIDVLVATTVVEVGVDVPNAT"
BASE COUNT          573 a         1270 c         1423 g          622 t
ORIGIN      
        1 gctgacttgg gggttgaccc ggtcgactgg gccctgcctt gacttggacg ctgccctaac
       61 cctcgccctg ctcacgctgc cagcaaccgg acccgttcag gagcgggtga ccttgcctgc
      121 cttgaggcag gaggtgcaga cattcaggcg tcggcgcgcg ctggcgccga tcttcgcccg
      181 gacggtctgg atgttcgggt tccaccggcg gttggtccgc cggtgcgagt gcgagaccga
      241 catgccaaag cccggtccct tgccgcagac atcgcacacg gcagccacgt cagacactcc
      301 ctctgagata ttcgtcgata ttcgtcgcga ggatttcccg gcaagggtag ccgggtgctg
      361 ggcggtgttg cacaccgggg acggcaccgg agatgagtag ccttcaacgc agccccgcac
      421 gaaggcggtg aggaggagcc gcagtgttgc agtcgttgga cggggtggcg atccgtcact
      481 gggctgccac ctgctgcgat gccctcgcgg cgcaccgtga cgagatcgac gctctcaacg
      541 tgttcccggt cgccgatttc gacaccggtt ccaacctctt ggccactatg cgagccgggt
      601 tggatgccgt gctccgcgat cacggtgacg accggtctcc gccgagcagc accgttgcgg
      661 tgctcgctcg aggtgcgctg atgggggcgc gaggcaactc cggagtcatc ctgtcccaga
      721 tactgcgcgg tctcgctgag gccccggtcg gtgcggcctt ggacggtgcg gcgctgcgag
      781 agggactccg ccgcgccggt gagctcgcta cggccgcggt gtccaatccg gtgtcgggga
      841 cggtgctgac cgtcctgcac gatgcggctc aggcggccat gggtgtccca tccgacgatc
      901 tcggcgacgt ggcggcagcg gccaccatcg ctgcggccgg agcgctggcc gaaacccctc
      961 gtcagctcga tgtgctggcc gcagcggggg tggtggacgc gggggggcgg ggtctggtcg
     1021 tgctgctgga ggcactgttg acggtggtca ccgagtgccg gggcatgggg ctgacggtca
     1081 ccgctgaaat ccccgcgcgc ccggcatcaa cacggcaagc gcctcatgag gttgggtcct
     1141 cggcctacga gtacgaggtg atgtacctcc tcgacggtac tgacgaggcc cgcgccggca
     1201 cattgcgtgc cgagctcgcc gtgctgggtg attgtgtggc agtcgtcagc tcgggcccgg
     1261 gggcggcagc gttgtggaag gtgcacgtgc actgttgcga tgtcggtgcc gcgatcgagg
     1321 caggtatccg agccggtcgg ccgcaccgca tcaccgtgct gcgcctcacc gaccggagct
     1381 gtgccgacca gcccggtgcc gaccagtccg atgttgtcca gcccggtgcc gaccagtccg
     1441 gcgcgacgac ggcgcagggc cgcttcatca cgggccacgc ggtgctcgcc atggtttccg
     1501 gttccggcct ggccgagctg ttccgcagcg agggcgcctt cactgcgtcc tccaccagca
     1561 gcgcgaccga gttgctcgcc gtcctcgcgg gcacccgggc ccggcacatc accgtgctgg
     1621 cgagtccggc cgccgcaagc cggattgcgg agatcacggt tgcacacgct cgggacaccg
     1681 gtcaggtggt ggtagtggtc ccgaccgcct ctgcggtgca ggctttagcg gcgattgcgg
     1741 tgcatgacgc cgggcggcca gcggccgagg acctgatggc catgaccgag gctgcggcag
     1801 ggacccggcg cggggagctg gcagtggcca gcgcagcggc gccgacgtgg gtcggctgcc
     1861 agccgggcga tgtcctcggc acggtgggtg acgaggttgt gttgaccgac tgtgattcat
     1921 tagctgccgc ccgggggttg gcggaggtga tgctctcgga gggcggggag ctggtgacgg
     1981 tgctgctcgg ccagcatgcg cccgacggcg tgggagacat gctgatcaag catcttcgcg
     2041 ccgtacatcc ggaggtcgag acggtggtct acgccgccga cgtgccggac agcatgctgt
     2101 tgatcggggt ggagtgagct gtggccgcga tggacgatgc cttggatcgg gtgatcggtg
     2161 ggaagccggc gaccctgctg gccgagcatc tcgacgttca caccatcggt gagctgctgc
     2221 ggcactaccc gcgtcggtac gcagaccaag gccagctcac cccgatcgcg ggtctgacac
     2281 ccggcgagga cgtcacggtc gtcgccgagg tccgtagctg cagctcacgg cagatgcaga
     2341 ccaagaaggg gacaatccag aacgcggtga tctgggacgg cagccgcact ttgaacctca
     2401 ccttcttcaa ccagtggcgc ctggacaaga cacttacccc cggccggaag gcgttgttcg
     2461 cgggacgggt gacgacgtac ggcaagaaac ggaccctgca gctcacacac ccgaggtacc
     2521 aactgctcga cggtgacgcc gatgagctcg cggagttctc ccggccactg attccggtct
     2581 atcccgccac caaggatctg ccgtcctgga cgatccagct ctgcgtacgc caggtgttcg
     2641 acgtgctgga cgaaccgctg gacccgttgc cgcagtcgct gcgcaagcag cacgggttgc
     2701 tggggctggg cgctgcgttg cgggccgtgc accgttccga tgaccggtcc gagatcgccg
     2761 cggcccaccg ccggttgaag tgggacgagg cgatggcggt gcagctcacc ctcgcgcagc
     2821 gccgccagga ggcggcgttg cggcccgcgc cggcctgccc acgccgagac ggcgggctgg
     2881 ctgccgcgtt cgacgccgca ctgcccttca ccttgaccgc cgggcagcga caggtcggcg
     2941 agctgctggc cgcggaactc gccagcgagc atccgatgaa ccggctgctg caaggcgagg
     3001 tcgggtccgg taagaccgtg gtggcgctgc gcgcgatgct gcaggtcgtc gacgccgggc
     3061 ggcaagcagt gctgctagcg cctaccgagg tgctcgccgg gcagcacgcc cggtccctgc
     3121 gcgacatgct gggtgacctc ggccgggctg gtgaactggg atcggccgac gttgccaccc
     3181 ggatcgtgct gctcaccggg tcgctgccgg cacgggcccg caagcaagcc ctaatggacg
     3241 ccgccagcgg tgcagcgggg atcgtggtcg gcacgcacgc cctgatccag gaccgggtgt
     3301 ccttcgccga tctgggcatg gtggtggtcg acgagcagca ccgcttcggc gtcgaacagc
     3361 gcgacgcgtt gcgcggcagg gcggccgacg gcaccagccc gcacgtgctg gtgatgaccg
     3421 cgacgccgat accgcgcacc gtcgcgatga ccgtctacgg cgacctggag gtgtgctcgc
     3481 tgcgcgagct gcccggtggc cgatcaccga tctcgaccac agtggtgccg gtcggcgaga
     3541 agcccagctg gctggaacgg gtctggcgac ggatcaccga ggagatcgcc gccggtaggc
     3601 aggcctacgt ggtatgcccc cgaatcggcg gcgaaattgg cggcgaggac gctgccggcg
     3661 aggacacctc tgacggggat ggcagcggac gcccgccgat tgcggtgctc gacctggtgc
     3721 cgcagctcac cgagggcccg ttggccgggc tgcggctggg ggtattacac ggccggctcc
     3781 ccggggagga gaaggacgcc gtgatgcgat ctttcaccgt cggcgagatc gacgtgctgg
     3841 tagccaccac cgtcgtcgaa gtcggagtgg acgtgccgaa cgccaccg
//
LOCUS       DATADN010000715         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_831537, whole genome shotgun sequence.
ACCESSION   DATADN010000715 DATADN010000000
VERSION     DATADN010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831537"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..263)
                     /locus_tag="VHH53_09280"
     tRNA            complement(191..263)
                     /locus_tag="VHH53_09280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Leu,seq:tag)
     gene            complement(300..773)
                     /gene="bcp"
                     /locus_tag="VHH53_09285"
     CDS             complement(300..773)
                     /gene="bcp"
                     /locus_tag="VHH53_09285"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX2290367.1"
                     /translation="MTDNVRLSPGDPAPDFALPDADGKPVSLSDYRGQSVVVYFYPAA
                     GTPGCTKQACDFRDNLAELDTAGFAVLGISPDPPVKLAKFRDAEGVHFPLLSDADRSV
                     LTAYGAYGEKQMYGKTVTGVIRSTFVIDAQGRIDKALYNVRATGHVAKLRKDLGV"
     gene            866..1087
                     /locus_tag="VHH53_09290"
     CDS             866..1087
                     /locus_tag="VHH53_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX2290368.1"
                     /translation="MARDADTIERDIEQARDALAATLDELGTRADPRRAIEAGKEQVE
                     TTMADPKVRYALMGVGGILVLAVLRKLFR"
     gene            complement(1122..2849)
                     /gene="treZ"
                     /locus_tag="VHH53_09295"
     CDS             complement(1122..2849)
                     /gene="treZ"
                     /locus_tag="VHH53_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX2290369.1"
                     /translation="MDFTVWAPQRQRVLVVVDGVEHDLVRTTGGWWGADVDGAGPGSD
                     YAFRLDDEDTALPDPRSRWQPHGVHAASRVYDHRAFTWTDQGWTGRALPGSVLYELHI
                     GTFTPGGSFDSAIERLDHLVDLGIDLVEVMPVNAFDGPRNWGYDGVDWYAVTENYGGP
                     DAFKRFVDACHGHGLGVVLDVVHNHLGPSGAYLERFGPYFAGSNIWGPSLNLDGPHSD
                     EVRRYVIDNALEWLRAYHLDGLRLDAVHALRDSRAIHVLEELAIEVDTLASHLRRPLS
                     LIAESDLNDPRLVTPRVAGGYGLTAQWDDDIHHCLHATLTGEQQGYYADFGALPDLAR
                     TLRGAFFHDGRWSQFRQRSHGRPVDTAQLPGWKFLAYLQNHDQVGNRATGDRISASLS
                     PGLLACGAALVLCSPYTLMIFMGEEWGAGTPFQFFSFMPDPELRDAIRRGRYAEFSQH
                     GWDDVDVPDPNDEQTFLRSRLDWSEAGKEPHATLLGIYRELIALRKRYPELSDPRLTQ
                     LAVDVDADARTLVLHRGRLRVAVNLSAQPVSIALGGQLTQVLLASGEATTTPAHVRLG
                     GESFAVVAL"
BASE COUNT          534 a          971 c         1057 g          492 t
ORIGIN      
        1 gctcggttcc atctgggttc ccactatcag gcctcatcgc cgaggcactg gtctatggca
       61 ggattgccgg ccatttcgcc gccaactggc tcactcttgg tgccgatgag aggtcgaatc
      121 aaccggcgga caaaaaaagc agtcctcccg taggtaagat agtggaaccc gctgtgctct
      181 gtaattgctg tgccggcggt gggattcgaa cccacactgg cgggttccta agacccgggc
      241 ctctgccatt gggctacgcc ggcttgcggt tacgcgcgca ccacgcatag ggtacggcgt
      301 cagactccga ggtctttacg gagcttggcg acgtggccgg tggcgcgcac gttgtacagc
      361 gccttgtcga tgcggccctg cgcgtcgatc acaaaggtgg accggatcac gccggtgact
      421 gtcttgccgt acatctgctt ctcgccgtag gcaccgtagg cagtgagcac tgagcggtcc
      481 gcatcggaca gcagcgggaa gtgcacgccc tccgcatcgc ggaacttcgc cagcttcacc
      541 ggcggatccg gcgagatccc gagcaccgcg aaaccggccg tgtccagctc agcgaggttg
      601 tcgcggaagt cgcaggcctg tttggtgcag ccgggggtcc ccgcggcggg gtagaagtag
      661 accaccaccg attgaccgcg gtagtccgac agcgagacgg gtttgccgtc ggcgtcgggc
      721 agggcgaagt caggagcggg gtcaccgggg gacagccgga cgttgtcggt cacgaggtcg
      781 gacgataccg ctggggatgg gggtggcctc atgacccggt tatccgtggc ctacggtgtg
      841 ccgcacaccc gcgcaaggag gactgatggc ccgggacgcc gacaccatcg aacgcgacat
      901 cgagcaggct cgtgacgcgt tggccgcgac gctggacgag cttggtaccc gggcggatcc
      961 ccggcgggcg atcgaggccg gcaaggagca ggtggagacc accatggcgg acccgaaagt
     1021 gcgttatgcg ctgatgggtg tcggtgggat cctcgtgctg gcggtgctgc gcaagctgtt
     1081 ccgctaggta gccgcaacgt cacagcgagg tagccacaac gtcacagcgc taccacagcg
     1141 aaggactcac cacccagccg cacgtgggca ggtgtggtgg tggcttcccc cgacgcgaga
     1201 agaacctggg tgagctgccc gcccagcgca atgctgaccg gctgcgcgct caggttcact
     1261 gccacgcgaa gtcggccacg gtgtagcacc agcgtgcggg cgtcggcgtc cacgtcgacg
     1321 gcaagctgag tcaacctcgg gtcagaaagc tctgggtagc gcttgcgcag cgcgatcagc
     1381 tcccggtaga tgcccagcag ggtcgcatgg ggctccttgc cggcttcgga ccagtccagc
     1441 cgggaccgca ggaaggtctg ctcgtcgttc gggtcgggca cgtccacgtc atcccacccg
     1501 tgttgggaga actcggcgta gcggccgcgt cgaatggcgt cgcgcagctc ggggtcgggc
     1561 atgaacgaga agaactggaa tggggtaccc gccccccact cctcgcccat gaagatcatc
     1621 agcgtgtagg gcgagcacag caccagtgct gcaccgcatg ccagcagacc gggcgacagt
     1681 gaggccgaga tccggtcgcc ggtagcgcgg ttaccgactt gatcgtggtt ctggaggtag
     1741 gcgaggaatt tccagccggg cagctgcgcg gtgtccaccg ggcggccgtg gctacgttgc
     1801 cggaactgcg accagcgccc gtcatggaag aacgcgccgc gcagcgttcg ggccaggtcg
     1861 ggcagcgcgc cgaagtcggc gtagtagccc tgctgctcac cggtcagggt ggcgtgcagg
     1921 caatggtgga tatcgtcgtc ccactgcgcg gtcagcccgt aaccgccggc gacgcgcgga
     1981 gtgaccagcc gcgggtcgtt gaggtcggac tcggcgatca gcgacagcgg ccgtcgcagg
     2041 tggctggcca gggtgtccac ctcgatggcc agctcctcca agacgtggat cgcgcggctg
     2101 tctcgcaggg cgtgcacggc gtccaacctc agcccgtcga ggtgataggc ccgcagccat
     2161 tccagggcat tgtcgatcac gtagcgacgc acctcgtcgg agtgcggtcc atccaggttc
     2221 agtgacgggc cccagatgtt gctgccggcg aaatagggac cgaagcgttc caggtaggcg
     2281 ccggatgggc cgaggtggtt gtgtacgaca tcgaggacca cgcccagccc atggccgtgg
     2341 caggcgtcca cgaaccgctt gaaggcgtcg ggcccgccgt agttctcggt gacggcgtac
     2401 cagtccaccc cgtcgtagcc ccagttgcgg ggcccgtcga aggcgttgac cggcatcacc
     2461 tcgaccagat cgatgcccaa gtcgacgaga tggtcaaggc gctcgattgc ggagtcgaag
     2521 ctgccccccg gggtgaaggt cccgatgtgc aactcgtaga gcacgctgcc gggcagcgcc
     2581 cggccggtcc agccctgatc ggtccaggtg aaggccctgt ggtcgtagac ccgggacgcc
     2641 gcatgcacgc cgtgtggctg ccaccgcgat cgtggatcgg gtagcgcggt gtcctcgtcg
     2701 tccagccgga aagcgtagtc ggaacctggc ccggcgccgt cgacatccgc accccaccag
     2761 ccaccggtcg tccggactag atcgtgctct acaccatcca ccacgaccag cacccgctgc
     2821 cgctgcggag cccacaccgt gaagtccata agcccgatct tgtcagggcc gggcaatatt
     2881 gtccggccaa accacggcga gggcacggca tgatcgcgtt ttatgtgccg ccgacgacat
     2941 ttgatgtagc tgacggcaca caaacagtgt caacagccga gtgattgtca gtcggccagt
     3001 agtagggcga cggggtagcg ggcgagtaga tcggccagcg gcacccgccc cgac
//
LOCUS       DATADN010000716         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1282838, whole genome shotgun sequence.
ACCESSION   DATADN010000716 DATADN010000000
VERSION     DATADN010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282838"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1210)
                     /locus_tag="VHH53_09300"
     CDS             complement(26..1210)
                     /locus_tag="VHH53_09300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX2290370.1"
                     /translation="MKAVAWHGKRDVRVDEVADPVIKEPTDVIVRITSSGICGSDLHL
                     YELFGPFMDEGDVLGHEPMGVIEEVGSGVTDLRPGDRVVVPFNISCGTCFMCDQGLQS
                     QCETTQVRDQGMGASLFGYTKLYGQVPGGQAQYLRVPFGNTLPIKVPEGPPDDRFIYL
                     SDVLPTAWQAVEYANVPPGGSVAVLGLGPIGDMCARIAQQRGAGQVFGIDLVPERLAR
                     ARERGVHTVDLREHDGDVTGPIRELTNGRGPDSVIDAVGMEAHGASAARLVQEMSTLL
                     PSGVAGNLMRKAGIDRLHALRVAIDLVRRGGTISIIGVYGGMLDPLPMMTMFDKQIQL
                     RMGQANVRRWVDDVLPLLTDEDPLGVDGFATHHVPLADAREAYMNFREKKEGHVKVLL
                     KP"
BASE COUNT          224 a          568 c          500 g          260 t
ORIGIN      
        1 ggtcgggccg gcgccccgga ggccgtcacg gcttgagcag gaccttgacg tggccctcct
       61 tcttctcccg gaagttcatg taggcctcgc gtgcatcggc gagcggcacg tggtgggtgg
      121 cgaagccgtc gacgccgagc gggtcctcgt cggtgagcag tggcaggacg tcgtcgaccc
      181 agcggcgcac gttcgcctgc cccatgcgca gctggatctg cttgtcgaac atggtcatca
      241 tcggcagcgg gtccagcatg ccaccgtaca cgccgatgat cgagatcgtg ccgccccgcc
      301 ggaccaggtc gatggccacc cgcagcgcgt gcagccggtc gatcccggcc ttgcgcatca
      361 ggtttccggc cacgccgctg ggcagcaacg tgctcatctc ctggacgagc cgggccgcgc
      421 tggccccgtg cgcctccatg ccgaccgcgt cgatcacgct gtccggcccg cggccgttgg
      481 tgagctcgcg gatcggcccg gtcacgtcgc cgtcgtgctc gcgcaggtcg accgtgtgca
      541 cgccgcgctc cctggccctg gccagccgtt ccggcaccag gtcgatgccg aacacctggc
      601 ccgccccgcg ctgctgggcg atccgggcac acatgtcgcc gatcgggccc agaccgagca
      661 ccgcgacgct gccacccggc gggacgttcg cgtattccac cgcctgccag gccgtgggca
      721 gcacgtcgga caggtagatg aaccggtcgt ccggcgggcc ttccggaacc ttgatcggca
      781 aggtgttgcc gaacggcacc cgcaggtact gggcctgccc gccgggcacc tggccgtaga
      841 gcttggtgta gccgaacagg ctggcgccca tgccttggtc acgcacctgg gtggtctcgc
      901 actgtgactg caggccctgg tcgcacatga agcaggtgcc gcaagagatg ttgaacggca
      961 ccaccacccg atcgcccggg cgcaggtcgg tcacgccgga accgacctcc tcgatgacac
     1021 ccatcggctc gtgtccgagc acgtcgccct cgtccatgaa cggtccgaac aactcgtaca
     1081 ggtgcaggtc cgacccgcag atcccgctcg acgtgatccg cacgatcacg tcggtcggtt
     1141 ccttgatgac cgggtcggcg acctcgtcca cccgcacgtc ccgcttgccg tgccacgcaa
     1201 cggccttcat gtgttcaccc cttcgctgtg tcactgctca accgggcagt cgttcccctc
     1261 gggggcctgg ctaaacgggt ttgggcccgc ggttggcggg tagccacgcc accggcgcca
     1321 tcgagggagg ctggacatgc cagatcgcga tcgcgagccc gccacggcgg agcccgagcc
     1381 ggagccggac ttcgccgatg agcacagccg gcccaccttc gctgacgaac cctgctactc
     1441 atggctgcct tcctcctgtc cgtcaaaagc ggcctggagc gtcgcccacg tctcaatctc
     1501 gtaagagcgt gggtcgtgat tccgaccttt ggggaccttc ttctccatct cc
//
LOCUS       DATADN010000717         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_398964, whole genome shotgun sequence.
ACCESSION   DATADN010000717 DATADN010000000
VERSION     DATADN010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398964"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VHH53_09305"
     CDS             <1..1009
                     /locus_tag="VHH53_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX2290371.1"
                     /translation="FAVALAGTGLLTTVGVIVAMDTFGPVSDNAQGIAEMSGDVKGEG
                     VGILTELDAVGNTTKAITKGIAIATAVLAATALFGSYRDAILSAAANLNLDAAFNVSV
                     PNVLVGLTIGAAVVFMFSGLAINAVSRAAGAVVYEVRRQFRDNPGIMDGSVRPEYGRV
                     VDICTKDSLRELTTPGLLAVLAPIAVGFGLGVGPLAGYLAGAIITGTLMAIFLANSGG
                     AWDNGKKLVEDGNHGGKGSDAHAATVIGDTVGDPFKDTAGPAINPLIKVMNLVSVLIA
                     PAIVAMSVGADASPSLRIGIAVAAVAVIVTAVVVSKRRSTSLVEPAPAETTAYSARRS
                     E"
     gene            1072..1641
                     /locus_tag="VHH53_09310"
     CDS             1072..1641
                     /locus_tag="VHH53_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290372.1"
                     /translation="MPSSRRRSGVIILPLFLALVGCGSGDAKGSGAAQPAVPQGSDPV
                     AWVGAFCSSSSGVVAGAAALGKAEETPQGQKDALLAFADSSQRAMADTARRLEQLGPP
                     RITDGKPLHDTAVKFFRSAAESVAGQRAKLAELDPNDPKFMENLSRLAGPDLGALGTQ
                     LQQMTTNPELAPAFNASAECQQLHSGSPR"
     gene            2006..>2207
                     /locus_tag="VHH53_09315"
     CDS             2006..>2207
                     /locus_tag="VHH53_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX2290373.1"
                     /translation="MVIVESPAKARKIASYLGSNYVVESSRGHIRDLPRGAADVPVKY
                     KGEPWARLGVDVDHSFEPIYVVS"
BASE COUNT          347 a          715 c          761 g          384 t
ORIGIN      
        1 gttcgcggtc gcgttggccg ggaccggact gctcaccacg gtcggcgtca tcgttgcgat
       61 ggacaccttc ggcccggtca gcgacaacgc ccaaggcatc gccgagatgt cgggcgacgt
      121 caagggtgag ggagtgggca tcctcaccga gctcgacgcg gtgggcaaca ccaccaaggc
      181 gatcaccaag ggcatcgcga tcgccaccgc ggtgctcgcc gcgaccgcac tgttcggctc
      241 ctaccgcgac gccatcctgt ccgcggcggc gaacctcaac ctggacgcgg cattcaacgt
      301 gtcggtaccc aacgtcctgg tcggcctgac catcggtgcg gccgtggtct tcatgttctc
      361 cggcttggcc atcaacgcgg tgtccagggc ggccggagcc gtcgtgtatg aggtgcgccg
      421 ccagttccgg gacaaccccg ggatcatgga cggctcggtc cggcccgaat acgggcgagt
      481 ggtcgacatc tgcaccaagg actcgctgcg tgagctgacc accccgggcc tgctcgcggt
      541 gctggccccc attgcggtgg gcttcggcct cggcgttggt ccgctcgccg gttacctggc
      601 cggtgcgatc atcaccggca ccctgatggc gatcttcctc gccaattccg gtggagcctg
      661 ggacaacggc aagaagctgg tcgaagacgg caaccatggc ggcaagggtt ccgacgcgca
      721 cgcggcgacc gtcatcggag acaccgtcgg cgacccgttc aaggacaccg caggcccggc
      781 gatcaacccg ctgatcaagg tgatgaacct ggtgtcggtg ctgatcgcgc cggcgatcgt
      841 cgcgatgtcg gttggcgcgg acgcttctcc cagcctacgg attgggatcg ccgtggcggc
      901 cgtagcggtg atcgtgacgg cggtggtggt gtccaagcga cggtccacgt ccctggtgga
      961 gccggcgccc gctgagacca ccgcgtattc ggcccgccgg tccgagtagc gtttcccgcg
     1021 ttaccggcgg tcgaaccgtc cgaccggtac ggacgttccc gaggagtagc tgtgccctca
     1081 tctcgtcgcc ggtccggcgt catcatcctg cccctgttct tggcgcttgt ggggtgcggt
     1141 tccggcgatg ccaagggctc cggtgcagcc cagccggccg tgccgcaggg ttctgacccg
     1201 gtagcctggg tgggcgcgtt ctgtagtagc tccagtgggg tggtcgccgg agccgccgcc
     1261 ctggggaagg cggaggaaac tccgcagggt caaaaggatg cgctgctcgc cttcgctgac
     1321 agctcccagc gagcgatggc tgacactgcc cgcaggctcg agcagctcgg cccgccccgg
     1381 atcactgacg gcaagccact acatgacact gcggtgaagt tcttcagaag tgcggccgag
     1441 tcggtcgccg gtcagcgagc gaagctcgcc gagttggacc cgaacgaccc gaagttcatg
     1501 gaaaatctca gccgccttgc tggtcccgat ctcggcgcgc ttggcaccca gctacagcag
     1561 atgaccacca acccagagct cgcccctgca ttcaatgcct cggccgagtg ccagcaattg
     1621 cacagtggca gcccacgctg agtcggggtt gcaaactcgc tgagctggtc ggaaggctgg
     1681 cgatgctgcg ccattcgggt tgcagccggg ctgatgtgac ccgcgtggcg taacgcagga
     1741 tgcggttacc cgcggcttca tgtgcgacgc tgactgcccc tgtgtgctgc gttgtgcaca
     1801 ctggtgcgtg gggatcgggg catcacgagt tcgtaaacga ggtaggtcga gcgtggcagg
     1861 gtcgaaaacg acggtaagta caggcggcgg gtccgccagc ggcaggcgcg gccgcgttgc
     1921 tgacgggtcc gctgataagg ccgctggtgg ggccgctcga aaggcaggat ccaacggcgc
     1981 tgcctccgat cgctcggccc gccgtttggt gatcgtggaa tcgcccgcca aggctcgcaa
     2041 gatcgcctct tacttgggtt cgaactacgt ggtggagtcc tcgcggggac acatccgcga
     2101 cttgccccgg ggggcagccg atgtgcccgt caagtacaag ggcgaaccgt gggcccggtt
     2161 gggggtggac gtcgatcact cctttgagcc gatctacgtc gtcagcc
//
LOCUS       DATADN010000718         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_692571, whole genome shotgun sequence.
ACCESSION   DATADN010000718 DATADN010000000
VERSION     DATADN010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692571"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1849)
                     /locus_tag="VHH53_09320"
     CDS             complement(<1..1849)
                     /locus_tag="VHH53_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX2290374.1"
                     /translation="MRAVLFTLGSGRAPRLFLTIHHLVIDGVSWRILLEDLENAYRQV
                     CAGHPAQLEPTGTSFRQWAHRLTEHVRSGGLNDDLTYWTAVSATAPADLPVDHAGCNT
                     VGSSSAVSVRLGRDDTDALLHRVPGVYRTQVNDVLLAAVGRVLSRWTGRDSVLVALEG
                     HGREELLDRVDLSRTVGWFTSMFPVALGVSTTSDWGDVLKSVKEQLRAVPHRGLSYAA
                     LRYLSTPDAPANALQADPAPQISFNYHGQWDGTAGSEGLYRGWCANIGQDFALDSTRT
                     HLLDVIGQVRDGELELSWLYSYEVHNETTVQRLAEEVIQALREIVEHCAAPGVGGRTP
                     SDFPLAHLDQPTVDRLVADGRGVEDIYPLTPLQIGMLFHSLVDASSGAYFEQVCLRLS
                     GVSDPQALGVAWQRVADRIPILRSRVVWDGVEQPLQVVHRQVTVPTTYYDWRELSQAE
                     ATEQQHQLVATDRATGMDLTTAPLLRVTIARLPSDETLLAWTSHHVLLDGWSLAEVLG
                     EVLEQYAAIVEDREARLVARRPFRDYVNWLYTQDQRQAQQYWRRVLSGVEAPTPLPYD
                     RAPIEAHRAESSNSVSIELPVEKSSWLQRVAKRNGLTVNSLVQGAWALLL"
BASE COUNT          367 a          645 c          552 g          325 t
ORIGIN      
        1 acaacaacaa cgcccaggcg ccctgcacaa ggctgttcac cgtcagcccg ttgcgcttag
       61 ccacccgctg cagccagctg gacttttcta cggggagctc gatggatacc gaatttgagg
      121 actcggcccg gtgagcctcg atcggcgccc ggtcataagg caagggagtg ggggcttcga
      181 cacccgacaa cacccgtcgc cagtattgtt gagcctgccg ttgatcttgg gtgtacagcc
      241 agttcacgta atctcggaat ggacgccggg ccaccagtcg cgcttcgcgg tcttcaacga
      301 tcgccgcgta ctgctccaaa acctcaccga gtacttcagc taggctccag ccatccagca
      361 aaacatggtg cgacgtccag gccagtaggg tctcgtcgct gggcaaccgg gcgatcgtca
      421 cgcgtagcag cggtgccgtg gtcaaatcca tgcccgtggc gcggtcggta gccaccagct
      481 ggtgttgttg ttctgttgcc tcggcttggg acagctcacg ccagtcgtag taggtagtgg
      541 gcaccgtgac ctggcggtgc accacctgca gtggttgctc cacaccgtcc cagaccaccc
      601 ggctacgcag gatggggatt cggtctgcca cccgttgcca ggccaccccg agcgcctgcg
      661 gatctgacac tcccgataac cgcaggcaca cctgctcgaa atacgcgccg gacgaggcat
      721 cgaccaggct gtgaaacagc atcccgattt gcagcggtgt cagcgggtag atgtcctcca
      781 ccccgcggcc atcagcgacc aaccgatcca ccgtgggctg gtccaagtgg gccagcggga
      841 agtccgacgg ggtccgcccc cccacccccg gtgccgcgca gtgttcgacg atttcccgca
      901 gcgcctggat gacctcctca gccagccgct ggacggtggt ttcgttgtgc acctcgtagg
      961 agtagagcca agacagctcc aactcgccat ctctgacctg gccaataaca tccagcagat
     1021 gtgtgcgggt gctgtcaagt gcgaagtctt gcccgatgtt ggcgcaccaa ccgcggtaaa
     1081 gcccctcaga gcccgccgtc ccatcccact gaccgtgata gttgaaggag atttgcggcg
     1141 ctgggtcagc ctgtagcgca ttcgccggcg cgtcgggtgt acttagatag cgcagagccg
     1201 cgtaactcaa tccccgatgc ggcactgctc gcaactgttc cttcaccgac ttgaggacgt
     1261 caccccagtc ggaggtcgtc gacacgccga gcgccacggg gaacatcgag gtgaaccacc
     1321 ccaccgtgcg ggacagatcg acccggtcga ggagttcctc ccggccgtgt ccctccaagg
     1381 cgaccagcac gctgtcgcgc ccagtccaac gtgacagcac ccgacccacc gcggccagta
     1441 gcacatcgtt gacctgggtg cggtacacac ccggcactcg atgcaacaac gcgtccgtgt
     1501 catctcgacc cagccgcact gacacggcgc tgctcgaccc caccgtattg cacccggcat
     1561 ggtccaccgg cagatcagcc ggcgcggtgg cagacaccgc agtccagtag gttaaatcgt
     1621 cattcagccc accggagcgg acgtgttcag ttaaccgatg tgcccactgc ctaaacgagg
     1681 tcccggttgg ctccagctgc gccggatggc ccgcgcaaac ctgccggtag gcgttctcca
     1741 gatcctccag aaggatccgc caggataccc catcaatcac caagtgatgg attgtcaaga
     1801 agagcctggg cgcacgcccg gaaccaagag tgaacagcac cgcccgcatc agtggcccac
     1861 gggtgatgtc caagctggtc tgacacgcc
//
LOCUS       DATADN010000719         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_399238, whole genome shotgun sequence.
ACCESSION   DATADN010000719 DATADN010000000
VERSION     DATADN010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399238"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VHH53_09325"
     CDS             complement(<1..624)
                     /locus_tag="VHH53_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2290375.1"
                     /translation="MSTTLLLDARLPSVAGATSLAIVAGTVAWAGDDSTARACFSDAD
                     EVLELHGALVTPAFVDAHVHATSAGLLASGLDLTGCATLAQCLDLIAQRARPGTVLWG
                     QGWDETSWPERRAPTRTELERASSGGLVYLSRVDGHSAVVSSGVLDRSTGVIGAPGWA
                     AEGPLTREAHHHARRVAQESITPGQRRDLQQAFLAAAAARGVAVVHEC"
     gene            complement(621..980)
                     /locus_tag="VHH53_09330"
     CDS             complement(621..980)
                     /locus_tag="VHH53_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290376.1"
                     /translation="MPPLRKPESRPQKSRSRHALEPPLGLSEITWQRIGADPRAARLV
                     RPVWSRLAELELAGHDPGALAALRYTLILHQPNRRGTCRACRWRGWPIRWWRRRWPCA
                     TWIQIRAELLGDGRAKP"
     gene            1283..2134
                     /locus_tag="VHH53_09335"
     CDS             1283..2134
                     /locus_tag="VHH53_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2290377.1"
                     /translation="MADRGDPSALRWLIGVELHNYRKRAGQTLAAAAKALGCSPGLVG
                     HFEKGLYFPQPEQIAGLLAFYGAPSWDVERLSSLSGRADQRTWLAPWNDVIPDWLRTY
                     VGLEGLASYEIAYSPLVLHALVQTQEYSIGATAGSARVRPDQVERLVSLRMERQRQLF
                     SDTGPLRFTSIIEESVLDRPIGGDATMRHQLEHLVELSERDNVEILVLPTAVGHHDGL
                     EGAFTVLHFEQAQSVGYIEYSDGAIYVQDQDQVAAYTRTAESLCSVALSKEESAQAIA
                     ARLTKLR"
     gene            2149..2508
                     /locus_tag="VHH53_09340"
     CDS             2149..2508
                     /locus_tag="VHH53_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HEX2290378.1"
                     /translation="MNNRGWRAGWRTSTRSSNGGNCVEVDFTAVGVKMRDSKARGTGP
                     IIDFSPTQWAAFLAESVCGLPSANGAVTVIHLNGTRVRSTAGGVTLHFTRAEWAAFVA
                     GARDGEFDYSQVATLVS"
BASE COUNT          425 a          853 c          897 g          430 t
ORIGIN      
        1 gcattcgtgc acgacggcga cgccacgggc ggccgccgcg gccaggaacg cctgctggag
       61 gtcgcgccgt tggccggggg tgattgactc ctgagcgacc cggcgagcgt ggtggtgcgc
      121 ttcgcgagtc aatggcccct cggccgccca accgggggcg ccgatcaccc cggtggatcg
      181 gtccaggact cccgaggaca ccacggcaga gtgcccgtcg acccgggaca ggtacaccaa
      241 gccgccgctg gacgctcgct ccagttcggt gcgggtgggt gcacgacgct ccggccatga
      301 cgtctcatcc cagccttgtc cccacaacac ggtgcccggt cgggcacgct gtgcgatgag
      361 gtcgaggcac tgcgcgaggg tggcgcagcc ggtgagatcc agtccgctgg cgagcaggcc
      421 ggccgaggtc gcgtgcacat gggcgtccac gaaggccggg gtaaccagcg cgccgtgcag
      481 ttccaacacc tcgtcggcat cgctgaagca agcccgggca gtgctgtcgt cccccgccca
      541 ggccaccgtt ccggcgacga tggccagcga agtggctccg gcgaccgacg ggaggcgcgc
      601 atcaagcaac agggtggtgc tcatggttta gctctaccat ccccgagcag ctcggcgcgg
      661 atctggatcc aggtggcaca cggccagcgg cgtcgccacc atcggattgg ccacccccgc
      721 catcggcagg ctcgacaggt tccgcgacgg ttaggttggt gcaggatcag cgtgtagcgc
      781 agcgcggcca gcgccccagg gtcgtgcccg gcgagttcca attcggcgag ccgtgaccac
      841 accggccgga ccaggcgcgc cgcgcgcggg tcagcaccga tgcgctgcca ggtgatctcg
      901 ctgagcccca gcggcggttc cagtgcgtgc cgtgaccgtg acttctgcgg ccgtgactct
      961 ggctttcgca atgggggcat ccgccgcggc gcccctctcc ttgctgtggt agatctcccc
     1021 ttgcatggag ggaccggacc gacgtcgggg gtgtcggccc ggtcccacca cttaccggcc
     1081 ggatgggagg cacccttgtc gccgctgtgt cgggagcggg actaggttcg gccacccgac
     1141 cgagatgcga ctcgcaatga gccgcatggc ttcaacccta actagagccc tagctagtgt
     1201 caaatcccga gagtagcgag tcgatgacct tgccctacca gcgtgccgaa taggctgttc
     1261 cgatcgcagg agggagtagc cgatggcgga tcggggcgac ccgtcggcgt tgcgctggct
     1321 gatcggcgtc gaactacaca attaccgcaa gcgggccggc cagacgctgg cagccgcggc
     1381 gaaggcgctg gggtgctcgc cgggcctcgt tggccacttt gagaagggtc tctacttccc
     1441 gcaacccgag caaatcgctg ggctgctggc cttctacggt gcgccgtcgt gggacgttga
     1501 gcggttgtca tcactgtccg ggcgggctga ccagcggacc tggctggcac cgtggaacga
     1561 cgtgatcccg gattggttgc ggacctatgt cgggttggag ggtctcgcct catacgagat
     1621 cgcctactcg ccactggtcc tgcacgcgct ggtccaaacc caggagtatt cgatcggggc
     1681 caccgccggg agcgcgcggg tccgtccaga tcaggtggag aggctggtca gcctccggat
     1741 ggagcgccag cgccagctct tcagtgacac cgggccgctg cggttcacct caatcatcga
     1801 ggagtcggtc ctggaccgcc cgatcggcgg cgatgcgacc atgcgccatc agcttgaaca
     1861 cctcgtcgaa ctctccgagc gggacaatgt ggagatcctg gtcctaccca ccgcggtcgg
     1921 tcatcacgac gggctcgaag gtgccttcac cgtgctgcac ttcgagcagg cgcaatctgt
     1981 cgggtacatc gagtattcgg acggagccat ctatgtacaa gatcaagatc aagttgcagc
     2041 gtatactcgg acggcggagt ctctatgttc cgtggcgttg tcgaaagagg agtctgccca
     2101 ggccattgcc gcgcggctca ccaagttgag gtaatccagg aggatggtat gaataaccga
     2161 gggtggcgcg ccggatggcg gacgtcgacc cgcagcagca acgggggtaa ctgcgtcgag
     2221 gtcgacttca ccgcggtcgg cgtgaagatg cgtgatagca aggcccgggg caccgggccg
     2281 atcatcgatt tcagcccaac ccagtgggca gccttcctgg ctgagtcggt gtgcgggttg
     2341 cccagcgcca acggcgcggt caccgtcatt cacctcaacg gcacccgggt acggtccacc
     2401 gccggaggag tgaccttgca tttcacccgt gctgagtggg ctgccttcgt ggccggagcc
     2461 cgggacggtg agttcgacta ctcccaggta gcgaccctgg tcagctgaag tggcgcctac
     2521 ctagcccgac tcgtggccag ggcggggcgc cgccggcggt tcatcgacga cctcgccctc
     2581 cacgacggcg cccggtgcga agcgc
//
LOCUS       DATADN010000720         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1002092, whole genome shotgun sequence.
ACCESSION   DATADN010000720 DATADN010000000
VERSION     DATADN010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002092"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHH53_09345"
     CDS             <1..264
                     /locus_tag="VHH53_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2290379.1"
                     /translation="RELVSRSVAVINSVRPAAGNVDLDRVATHFAARCRSVCRMPFDE
                     HLEEGGEIELDRLSSAARLAALELAATVAEDFPHAVSRGRAFR"
     gene            complement(274..441)
                     /locus_tag="VHH53_09350"
     CDS             complement(274..441)
                     /locus_tag="VHH53_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290380.1"
                     /translation="MQYIMAAIGMAAVTALLWKAFGPQRPAARSVLAPDDDPEFLRGL
                     NRKRPEPGDER"
     gene            complement(594..773)
                     /locus_tag="VHH53_09355"
                     /pseudo
     CDS             complement(594..773)
                     /locus_tag="VHH53_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
     gene            966..1271
                     /locus_tag="VHH53_09360"
     CDS             966..1271
                     /locus_tag="VHH53_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HEX2290381.1"
                     /translation="MGAPQIPPESPLSGSALARDVALYSGARLALVAVLALLLVLAGV
                     PLLVAVLLALVLALPLSMVLLRSLRVRVNAGIAAAGQRRRSERDRLRNELRGEQDLR"
     gene            complement(1297..2172)
                     /locus_tag="VHH53_09365"
     CDS             complement(1297..2172)
                     /locus_tag="VHH53_09365"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEX2290382.1"
                     /translation="MATVAQWVEGARPRTLPNAIAPVLAGWGAAVAPVEQFSGAPRAL
                     LALVVALALVIGVNYANDYSDGIRGTDATRVGPMRLVGSGAAAPSAVRTAALVSFAVA
                     AVAGLVLVALAGQWWLLALGGACIAAAWFYTGGRRPYGYAGLGELAVFLFFGPVGVLG
                     TQYVISGQITPTGGFAAVAVGAMSAAVLVANNLRDIPTDAAAAKRTLAVRLGDHRTRM
                     LYTGLVAAPFVVTLGFAVTRWITLAGLLALVLALPALRRVRGGASGPALIPVLRDTGL
                     ALLVWAAATGAALAV"
     gene            complement(2183..>3167)
                     /locus_tag="VHH53_09370"
     CDS             complement(2183..>3167)
                     /locus_tag="VHH53_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290383.1"
                     /translation="LTAAHRLAEPRNQVVAELVADQPAGSTVAIDVRGTRENGSWTEW
                     VPASTEEPAVFDAAVMTVQTRVLLRGGDGGASPRVRSVRLSAHTVAQTLAALPLSARS
                     YRVFATREGLVGGTTANGHVIRPRDHFVALPSRRALAPRGSGDYTVKVCSDHGRCEWA
                     PVWDVGPWNTTDDYWNAPDARQSWRDLPQGRPQAQAAYSDGHNGGLDQYDRQVRNPAG
                     IDLADGTFWDGLGLRNNSWVTVTYLWADGGPLGIVRMPLLHVRSGPGTHHPPVGLAAQ
                     RATLRVECAVIGQAVTGTHGVTDRWLRIGTQQYVSAAHVALQGGGAPCTTP"
BASE COUNT          476 a         1086 c         1129 g          476 t
ORIGIN      
        1 cgggagctag tcagccggtc ggtcgccgtg atcaactcgg tacggccggc tgcgggcaac
       61 gtggacctgg atcgggtcgc cacgcacttc gctgcccgtt gccggtcggt ctgccggatg
      121 ccctttgacg agcaccttga agagggcggc gagatcgaac tcgaccggct cagttccgct
      181 gctcggctgg ccgccctgga gctggcggcg accgtcgccg aggacttccc gcacgcagtt
      241 tcccgcggcc gcgcctttcg ttagccgggc acgtcaacgc tcgtcgccgg gctcgggacg
      301 tttccggttc aagcctcgga gaaactccgg atcgtcatcg ggcgccagca ccgagcgtgc
      361 ggccgggcgc tgcgggccga atgctttcca cagcagcgcg gtgaccgcag ccatcccgat
      421 tgccgccatg atgtactgca cgccagccac ctcctcaggt gccaccgagc gtagtggcgt
      481 tcagcgcagc agacgagcag acctcacccc gatttcaccg cgccggacct tacggtgtcg
      541 gatctcaagg cgcgacctgg caggtcagct cgatccggcg gcgcaggggt ggtgagcgac
      601 gccagcaatg cgcggacttc ggtttcgcgg aaccgccggt gcccgccggg ggtcctgatc
      661 gagccaattc gtccagcgga tgcccatcgg gtgacggttt tgggatccac tcggaacagt
      721 gcggcaacct ccccgggcgt gagtagtcgc tcagtggctg atagctggga catcgcgggc
      781 ctccccagac tcgtcgatct cacagtggga tctttcgggc ttatcgtgac aggtcgtgcg
      841 ccaggtactc gaacggttgt ggcgaggtaa agcgccggta atgggaggaa ccgtgccaat
      901 ccctgccacc gtggagtcag gatggctgga gcgatgcagc gacgggtgtt ttaggctaaa
      961 cggtcgtggg cgcaccccag attcccccgg agagtcccct gtccggctcc gcgctggccc
     1021 gcgacgtcgc gctctacagc ggggctcgac ttgccctggt ggccgtgctg gcgctgctgt
     1081 tggtgctggc cggggtgccg ctgctggtcg cggtgctgct ggcgctggtg ttggcactgc
     1141 cgctgtcgat ggtgctgctg cgctcgttgc gggttcgggt gaacgctggg atagcagcgg
     1201 ctgggcagcg ccgccggtcg gagcgtgacc ggcttcgcaa cgagctccgt ggcgagcaag
     1261 atttgcgttg atccgcgagc gacgagtcgg ctggactcag acggccagcg ccgcaccagt
     1321 ggcagccgcc cacaccagca gcgccagccc ggtgtcccgc agcaccggga tcaatgctgg
     1381 cccactggcc ccaccacgga cccggcgaag cgcgggcagt gcgagcacca gggcgagcag
     1441 gccggccagc gtgatccagc gggtgaccgc aaagccgagg gtcaccacga agggggcagc
     1501 caccaacccg gtgtagagca tgcgggtccg gtggtcaccg aggcgcacgg caagggtgcg
     1561 tttggctgcg gcggcatcgg tggggatgtc gcgcaggttg ttggccacca gcactgcggc
     1621 tgacatcgct cccaccgcga cggccgcgaa accgccagtc ggcgtgatct ggccactgat
     1681 gacgtactgg gtgccgagca ccccaaccgg tccgaagaac aggaagacgg ccaactcgcc
     1741 gagaccggcg taaccgtagg gacggcgccc accggtgtaa aaccacgctg cggcaatgca
     1801 cgccccaccc agcgccagca gccaccactg tccggccagc gcgaccagca cgaggcccgc
     1861 gaccgcagcg accgcgaagc tgacaagtgc cgcggtacgc accgcgctgg gtgccgcggc
     1921 accggatccg accagccgca tcggcccgac ccgggtggcg tcggtgcccc ggatgccgtc
     1981 ggagtaatcg ttggcgtagt tgaccccgat caccagcgcc agggctacga ctagtgccag
     2041 cagggcgcgt ggcgcgccgg agaactgttc gaccggtgca accgccgcac cccagccggc
     2101 cagcaccggg gcgatcgcat tgggcaatgt ccggggtcgc gctccctcca cccactgcgc
     2161 cacggtcgcc acaggcgaga ccttacggcg ttgtgcaggg cgcgccgccg ccttggaggg
     2221 cgacatgtgc ggcggacacg tactgctgcg tgccgatccg cagccaccgg tcggtcacgc
     2281 cgtgggtgcc ggtgacggcc tgcccgatga cggcgcactc gacgcgaagc gtggcgcgct
     2341 gggccgccag gcctaccggc gggtgatggg tgccagggcc gctgcggacg tgcagcagcg
     2401 gcattcgcac gatgcccagt ggcccgccgt cagcccacaa ataggtgacg gtgacccagc
     2461 tgttgttgcg tagccccaga ccgtcccaga acgtgccgtc ggccaggtcg atcccggctg
     2521 ggttgcgcac ctgccggtcg tattggtcca ggcccccgtt gtggccgtcg ctgtaggcgg
     2581 cctgcgcctg cggccggccc tgcggcaggt ccctccagga ctgtcgggcg tccggggcgt
     2641 tccagtaatc gtcggtggtg ttccacgggc cgacgtccca caccggcgcc cattcgcagc
     2701 ggccgtggtc cgagcagacc ttcaccgtgt agtcgcccga accgcggggg gccagtgccc
     2761 ggcgcgaggg tagtgcgacg aagtggtcgc gcgggcggat cacatgaccg ttggcggtgg
     2821 tgcccccgac caggccctcg cgggtcgcga acacccgata gctgcgggct gagagcggga
     2881 gagcggccag ggtctgggcg acggtgtgcg cgctgagccg caccgatcgc acccgcgggc
     2941 tcgcgccgcc gtcaccaccg cgaagcagaa ctcgggtctg taccgtcatg accgcagcgt
     3001 cgaacaccgc gggttcctcg gtgctggcgg gcacccactc ggtccacgat ccattctccc
     3061 gggtgccgcg gacgtcgatg gccacggtcg acccggccgg ctgatcagcg acgagttccg
     3121 ccaccacctg gttgcggggc tcggcgagcc ggtgcgccgc agtgagc
//
LOCUS       DATADN010000721         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_74270, whole genome shotgun sequence.
ACCESSION   DATADN010000721 DATADN010000000
VERSION     DATADN010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74270"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VHH53_09375"
     CDS             <1..1213
                     /locus_tag="VHH53_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2290384.1"
                     /translation="LAGMRVVEGSAFVAAPLGGMTLAQLGADVIRFDPIGGGLDATRW
                     PVTADGKSLFWAGLNKGKRSIAVDLRSPLGKELLTDLITAPGDEAGLFLTNFPAAGWL
                     DFNKLTSRRPDLIMVNIVGNPDGSSAVDYTVNPATGFPWATGPSNLSDPLNHLLPAWD
                     AITGTLAATGLLAAERKRRVDGVGQSVRLALADVAFAMVGHLGKIAEVQINKHERPKY
                     GNYLYGAFGRDFVTKDGKRVMVVGLTARQWRSLVAAVGIADACVQVEKLLEVDLGKEG
                     DRFTARHVIGALVEPWVSSRTLSEVRKIFDAHEVCWGPYQTFAELVTTDPRCSPSNPM
                     FAAVDQPGIGTYLMPGSPLDFSAADRVRPAAAPQLGQHTDEILAEILGLSASEIGRLH
                     DEGVVAGIPDP"
     gene            complement(1522..2763)
                     /locus_tag="VHH53_09380"
     CDS             complement(1522..2763)
                     /locus_tag="VHH53_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2290385.1"
                     /translation="MSTARLLLELGAVLFALGACGRLAGRFGLSPIPLYLLAGLAFGT
                     GGLLPLEAPEGFFATGSQVGVVLLLLVLGLEYTADELVTNLRRQAPVGVLDLLLNGIP
                     GAALALMLGWGPIATLAMFGVTAVSSSGIVSKVLTDLGRLGNRETPSILSVLVIEDLA
                     MAVYLPVLTALVAASTLGSIASSVLIALGMLAVVLTVALRHGDAVTRFVGARSDEVLL
                     LRVLGLALLVAGAAEALHVSSAVGAFLLGIALSGEVADHIRGLLGPLRDVFAAVFFVF
                     FGLSTDPAALPSVLVPAALLAVVGVATKVLTGWAAGRRAGIGPAGRLRAGVTLVARGE
                     FSIIIAGLVAASVTPAFAPTVAAYVLIMAVLGPLAPRLGDPLVHHLAQREAKRRTADH
                     RTEKRSADPRPERLTGPADSA"
     gene            complement(2804..>3344)
                     /locus_tag="VHH53_09385"
     CDS             complement(2804..>3344)
                     /locus_tag="VHH53_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HEX2290386.1"
                     /translation="LAYRLGGTCKQVKRRVVMEVEVEETRLPGIGLRHDFVTAQGRRI
                     GVITQRNGVRHVALYDEEDSDACEATIELTSEESEVLAELLGAPRITERFARLREQVE
                     GLVTEGLPITAGSPFVHGTLGDTAIRTRTGASVVAVLRGDDVFASPTPDFRFQPGDRL
                     IVVGTKDGVHAAHALLASG"
BASE COUNT          596 a         1117 c         1119 g          512 t
ORIGIN      
        1 cctggcggga atgcgggtcg tcgagggctc ggccttcgtg gcggcaccgc tgggcgggat
       61 gacgctggca cagctcggcg cggacgtgat ccggttcgat ccgatcggtg gtggcctgga
      121 tgccacccgc tggccggtca ccgccgacgg caagagcctg ttctgggcgg gcctgaacaa
      181 gggcaaacgc tcgatcgcgg tcgacctgcg atcgcccctg ggcaaagagc tgctcaccga
      241 cctgatcact gcacccggcg atgaggcggg gctgttcctg acgaacttcc cggccgcggg
      301 ctggctggac ttcaacaagc tcacgtcccg gcgccctgac ctgatcatgg tcaacatcgt
      361 cggtaaccct gacggctcgt ccgcggtcga ttacacggtc aaccccgcca ccggcttccc
      421 ctgggccacc ggtcccagca acctgtccga cccgttaaac cacctgctgc cggcctggga
      481 cgcgatcacc ggcacgctcg cggcgaccgg gctgctggct gccgagcgca agcgacgggt
      541 cgatggggtc ggccagtccg tccggctcgc gctggctgat gtggcgttcg ccatggtcgg
      601 gcacctcggg aagatcgccg aagtgcagat caacaagcat gaacggccga aatacggtaa
      661 ctacctgtat ggagcgttcg ggcgtgactt cgtaaccaag gacggcaagc gggtgatggt
      721 agtcggcctg accgcgcggc agtggcggag cttggtggcc gcggtcggca ttgctgatgc
      781 ctgtgtccag gttgaaaagc tgcttgaggt cgatctgggc aaggagggtg accggttcac
      841 cgcgcggcat gtgatcggcg cgctcgtcga gccgtgggtc agtagtcgca cgctgtccga
      901 ggtccgaaag atcttcgacg cccacgaggt gtgctggggg ccgtatcaaa cattcgcaga
      961 gctggtcacg acggatccgc gctgctcgcc gagcaacccg atgttcgccg cggtcgatca
     1021 gcccggcatc ggtacttacc tgatgcccgg ttcgccgctc gatttctccg ccgcagaccg
     1081 ggtacggcct gcggcggcgc cccagcttgg acagcacacc gatgagatcc tcgccgaaat
     1141 ccttggcctg tccgcctccg agatcgggcg attgcacgac gaaggtgtcg tcgctgggat
     1201 cccagacccg taagcccagt cgcatgacag aaatggcccc ggcaagggag tgaactgatg
     1261 tttgcctttt actcgaatcg gctgggttgc cttggctccc tgctgatatc agccctgctg
     1321 acgaccctgc taatcttgat ctttttcgtc ctgtgagtcg gcctgcggca ggctggcgtt
     1381 ggtgtggcct gggcgtcccg gcgcggcgcc caagcgtaca gataaccact acggtttcgc
     1441 gccatgccat ccggcacatg agggttgtcc ctacgagacc ctggttggcg ccatgacccg
     1501 tgtgaccttc cgcgggtgtt cttatgcaga atccgcaggg ccggtaagac gttcgggcct
     1561 agggtcagca gacctcttct ccgtccggtg atcagcggtc cggcgtttgg cctctcgctg
     1621 ggcgaggtga tggacgagtg gatcgcccag gcgcggcgcc aacggtccga gcacagccat
     1681 gatgagcacg taggccgcca ccgtgggggc gaaggccggt gtcacagagg cggccacgag
     1741 gccggcgatg atgatggaga actctccccg ggcgaccagc gtgacgccgg cccgcagccg
     1801 tccggccggg ccaatcccgg cgcgccgtcc ggcggcccag ccggtgagca ccttcgtcgc
     1861 gacgccgacg acggcaagca gcgcggcggg caccagcacg gacggcagcg cggccggatc
     1921 ggtggacagc ccgaagaaga cgaagaacac cgcggcgaag acgtcacgca gcggaccgag
     1981 caagccccgg atgtggtcgg cgacctcgcc ggacagcgcg ataccgagca ggaacgcacc
     2041 gactgcggat gacacgtgca gcgcctcggc ggccccagcg accagcagcg cgagcccgag
     2101 cacgcgcagc agcagcacct cgtcggatcg ggcgcccacg aaccgggtca ccgcgtcgcc
     2161 gtggcgcaat gcgacggtca gcaccaccgc gagcatgccc agcgcaatga gtaccgagct
     2221 tgcgattgag cccagcgtgg aggcagcaac cagagcggtg agtaccggaa ggtacacggc
     2281 catcgcgaga tcttcgatca ccagaacgct cagtatcgac ggggtttccc ggttgcccag
     2341 ccggccgagg tcggtgagaa ccttggagac gatgcccgac gagctgacgg ccgttacccc
     2401 gaacatcgcc agcgtggcga tcggtcccca gccaagcatg agtgcgagcg cggcacccgg
     2461 aataccgttg agcaacagat caagcacgcc gacgggcgcc tgtcgacgca agttggtgac
     2521 cagctcatcg gccgtgtatt ccaagcccag caccagcagc aggaggacga cgcctacctg
     2581 ggagccggtg gcgaagaaac cctcgggtgc ctccagaggc agcaggcctc cggtgccgaa
     2641 ggccagccca gcgagcaagt aaagcgggat gggggatagc ccgaagcgcc cggccagccg
     2701 gccgcacgcg cccagcgcga acaggacggc gccgagctcc agcagcaggc gggcggtgct
     2761 cacccggtcc cgacagaccg gggcctcagt ccgggactac ccatcagccc gaggccaaca
     2821 gggcgtgagc cgcgtggacg ccgtccttgg tacccaccac gatgaggcgg tcgccgggct
     2881 gaaaacggaa gtccggggtc ggtgacgcga acacgtcgtc accgcgcagc accgctacga
     2941 ctgaagcgcc agtgcgggta cggatggcgg tatctccaag tgtcccgtgg acgaacgggg
     3001 agccggcagt gataggcaat ccctccgtga ccagcccttc aacctgctcg cgcagccggg
     3061 cgaatcgctc ggtgatccgc ggtgcaccga gcagctccgc cagcacctcg ctctcctccg
     3121 aagtcagctc gatcgtggcc tcgcacgcgt cggagtcttc ttcatcgtaa agagccacgt
     3181 gccggacgcc attgcgctgc gttatcaccc cgatccggcg tccctgcgcg gtgacaaagt
     3241 cgtgccgcag cccgatccca ggcagccgcg tctcctcgac ctcgacctcc atgacaaccc
     3301 tccgcttgac ttgcttacag gtgccgccta gcctgtaagc aagt
//
LOCUS       DATADN010000722         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_399888, whole genome shotgun sequence.
ACCESSION   DATADN010000722 DATADN010000000
VERSION     DATADN010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399888"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /gene="secY"
                     /locus_tag="VHH53_09390"
     CDS             <1..1042
                     /gene="secY"
                     /locus_tag="VHH53_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2290387.1"
                     /translation="RFEQLKKEGQSGQGKLTQYTRYLTIGLAILQATGIVAMANSGQL
                     FGNCVDEAGNQIPIIPQNSVFDLVLIVTAMTAGTAVIMWFGELITERGIGNGMSLLMF
                     VNIAARIPFEGVNILNNDETGGLIFAVVCAFGLLIIASVVFVEQGQRRIPVQYAKRMI
                     GRRMYGGTSTYLPLKVNQAGVIPVIFATSLLYLPDLISRLIGTDANSWVQRFINDHLA
                     RQSSPVHIAVYFAMIIFFTYFYITITFNVNERADEMKKFGGFIPGIRPGRPTAEYLSY
                     VLGRITLPGSIYLGVIAVLPNFFLDITGEGNNQNFPFGGTAVLIMVGVGLDTVKQIES
                     QLMQRNYEGFLR"
     gene            1039..1593
                     /locus_tag="VHH53_09395"
     CDS             1039..1593
                     /locus_tag="VHH53_09395"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2290388.1"
                     /translation="MTRVVLVGPPGAGKGTQAATLSDKLGVSHISTGELFRAHIDAET
                     ALGRTVKGYLDAGQLVPDEVTNEMVRARLDESDVEVGFLLDGFPRNVGQANLLHKILA
                     EANHRLDAVIELQVDEDVLVRRLLARGRADDTEDVIRHRQQVYRSETAPLLDYYHDLL
                     ITVDGTGSVDEVTNRILSALRDRK"
     gene            1650..2423
                     /gene="map"
                     /locus_tag="VHH53_09400"
     CDS             1650..2423
                     /gene="map"
                     /locus_tag="VHH53_09400"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2290389.1"
                     /translation="MIEIKTSTELEAMRAAGLVVARTLALLAEAVQPGVTTAQLDELA
                     EQSIRDAGAIPSFKGYHGFPASICASVNEQVVHGIPSAGQVLADGDLISIDCGAILDG
                     WHGDASVTVAVGSVSAEDLALTEATRRSMLAGIEAIQPGGRLTDISYAVESATRRAER
                     DHGRSYGIVRDYGGHGIGRAMHMEPFLPNYGRPGRGTRLRVGMALAIEPMLTMGSART
                     EELSDGWTVVTTDGSRAAHWEHTVAITDSGPWVLTAPPD"
BASE COUNT          404 a          845 c          799 g          386 t
ORIGIN      
        1 gcgcttcgag cagctgaaga aggaaggcca gtccggccag ggcaagctga cccagtacac
       61 ccgctacctc accatcgggt tggcgatcct gcaggccacc ggcatcgtcg ccatggccaa
      121 cagcgggcag ctgttcggca actgcgtcga cgaggccggc aaccagatcc cgatcatccc
      181 gcagaacagc gtcttcgacc tggtcctcat cgtcaccgcg atgaccgcgg gcacggccgt
      241 gatcatgtgg ttcggcgagc tgatcaccga gcgtggtatc ggcaacggca tgtcgctgct
      301 gatgttcgtc aacatcgcgg cgcggatccc gttcgagggt gtcaacatcc tcaacaacga
      361 cgagaccggc ggcctcatct tcgccgtggt ctgcgcgttc gggctgctga tcatcgccag
      421 cgtggtgttc gttgagcagg gccagcgccg catcccggtg cagtacgcca agcggatgat
      481 cggtcgccgg atgtacggcg gcacctccac gtacctgccg ctgaaggtca accaggccgg
      541 cgtcatcccg gtcatcttcg cgacgtcgct gctctacctg cccgacctga tctcgcgcct
      601 gatcggcaca gacgcgaaca gctgggtcca gcggttcatc aacgaccacc tggccaggca
      661 gtccagcccg gtgcatatcg cggtgtactt cgcgatgatc atcttcttca cgtacttcta
      721 catcacgatc acgttcaacg tgaacgaacg tgccgacgag atgaagaagt tcggtgggtt
      781 cattccgggc attcgccccg gccggcccac ggccgagtat ctgagctacg tgctgggccg
      841 gatcaccctg ccagggtcga tctacctcgg tgtcatcgcg gtgctgccga acttcttcct
      901 ggacatcacc ggtgagggca acaaccagaa cttcccgttc ggtggaaccg ccgtactgat
      961 catggttggc gtcggccttg acaccgtcaa gcagatcgag agccagctga tgcagcgcaa
     1021 ctacgaaggg ttcctcagat gacgcgagtc gttctcgtcg gcccgccggg cgccggcaag
     1081 ggcacccagg ccgcgacgtt gagcgacaag ctcggggtgt cccacatctc caccggggag
     1141 ttgttccgcg cgcacatcga cgccgagacc gcgctcggcc gcacggtgaa gggctacctc
     1201 gacgccggcc agctcgttcc ggacgaggtg accaacgaga tggtgcgggc tcggctggac
     1261 gagtcggacg tcgaggtcgg cttcctgctg gacgggttcc cgcgcaacgt cggccaggcc
     1321 aacctgctgc acaagatcct cgccgaggcc aaccaccggc tcgacgcggt gatcgagctg
     1381 caggtcgacg aggacgtcct ggtgcggcgg ttgctggcgc gtggccgcgc cgacgacacc
     1441 gaggacgtca tccggcaccg gcagcaggtg taccgctcgg agaccgcacc gctgctggac
     1501 tactaccacg acctgctcat caccgtggac ggcaccggct ccgtcgacga ggtcaccaac
     1561 cggatcctga gcgcgctgcg cgaccggaag tgactcgtgg tgggctgctc agcgggctgc
     1621 ggctcggact ccagcagcgg cgtgaccgga tgatcgagat caagaccagc accgagctgg
     1681 aggcgatgcg ggccgcgggc ctggtcgtcg cccgcaccct cgccctgctg gccgaggccg
     1741 tccagccggg ggtgaccacc gcgcagctcg acgagctggc cgagcagtcg atccgcgacg
     1801 ccggcgcgat cccctcgttc aagggctacc acggctttcc ggccagcatc tgcgcctcgg
     1861 tcaacgagca ggtggtgcac ggtatcccga gcgcgggtca ggtactggcc gacggtgatc
     1921 tgatctcgat cgactgcggc gccatcctgg acggctggca cggcgacgcc tccgtcaccg
     1981 tcgcggtcgg ctccgtcagc gccgaggacc tggccctgac cgaggccacc cgccggtcga
     2041 tgctggccgg tatcgaggcc attcagcccg gtggccgcct caccgacatc tcctatgccg
     2101 tcgagtccgc gacccggcgc gccgagcgtg accacggccg cagctacggc atcgtccggg
     2161 actacggcgg gcacggcatc ggccgggcca tgcacatgga gccgttcctg cccaactacg
     2221 gcagacccgg gcgcggcacc cgcctgcggg tcgggatggc gctggccatc gagccgatgc
     2281 tcaccatggg ctcggcccgg accgaagagc tgtccgacgg ttggaccgtg gtcaccaccg
     2341 acggttcccg ggcggcgcac tgggagcaca cggtggccat caccgacagc gggccgtggg
     2401 tgctcaccgc cccgcccgac tgaccggtcc gcgg
//
LOCUS       DATADN010000723         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1153145, whole genome shotgun sequence.
ACCESSION   DATADN010000723 DATADN010000000
VERSION     DATADN010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153145"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VHH53_09405"
     CDS             <1..911
                     /locus_tag="VHH53_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2290390.1"
                     /translation="AHCAPAVITDDKRADPRYLYIPELRGEDFTSMASVPMITRPGDL
                     VGVLNVHTHDRREFTNADIELLGTVAGLMAGAIENASLHRKLAEREEAMERFAEQIVL
                     LQETERRCLAGEIHDGISQRIVSLSFHLSAAADAVASDPGGAADQIARAQELAAGALD
                     ETRQAIAGLRPPVLDDLGLAASLESLARSTPLPDVQVDTVPTSLPEHVETAVYRIAQE
                     ALQNIMKHANAGHVHLRLSATAGAVLLEISDDGTGFDPATTVERVGPVGYGLPGMQQR
                     AELLGGQLTADSSPGRGTVVRLRIPI"
     gene            1044..1271
                     /locus_tag="VHH53_09410"
     CDS             1044..1271
                     /locus_tag="VHH53_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290391.1"
                     /translation="MTTVEEDRTQARIDGLLQVLRDAESDFRRSYARVLDVVAELDAE
                     RAGAVTGFGTTARLLAGALNPTKGEAVDGGR"
     gene            1356..1760
                     /locus_tag="VHH53_09415"
     CDS             1356..1760
                     /locus_tag="VHH53_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX2290392.1"
                     /translation="MPHYRMSEIAELFGVSDDTVRRWADSGQLTAIRDAGGRKAVDGA
                     ELAAFAKSQAHATPDPSLVDRSARNRFVGLVTEVICDLVMAQVEIQCGPHRVVSLMSS
                     EAVRELGLAPGVLAVAVVKSTEVMVETPGGRA"
BASE COUNT          312 a          538 c          600 g          310 t
ORIGIN      
        1 ccgcacactg cgcgccggcg gtgatcactg acgacaagcg cgccgatccg cgatatctct
       61 acattcccga gctacgcggg gaagacttca cgtcgatggc ctcggtgccg atgatcaccc
      121 ggcctggtga tctcgtgggc gtcctcaacg tgcataccca cgaccgccgc gaattcacca
      181 atgccgacat cgagctgctg ggcactgtgg cgggtctgat ggccggggcc atcgagaacg
      241 cgagcctgca ccgcaagcta gccgagcgag aggaggcgat ggagcggttc gctgagcaga
      301 ttgtgctgct ccaggagacc gagcggcggt gccttgccgg cgagattcac gatggaatca
      361 gccagcgcat tgtcagcttg tcgtttcacc tctcagcggc ggcggacgcg gttgcctccg
      421 accccggcgg tgccgccgac cagatagccc gtgctcaaga actcgccgcc ggagcgctgg
      481 atgagacccg gcaggcgatc gccggactgc gcccacccgt gctcgacgac cttggattgg
      541 ccgccagcct ggaaagcctt gcccggtcga ctccgctacc cgacgtccag gtggacaccg
      601 taccgaccag cctgcccgag cacgtcgaga cagcggttta ccggatcgcc caagaggcgc
      661 tgcagaacat catgaagcat gccaacgctg ggcacgttca cctccggctc tcggcgaccg
      721 ccggcgcggt gttgctggag atttccgacg acggcaccgg cttcgacccg gccaccacag
      781 tcgagcgggt tggcccggtg ggttacgggc tgccaggtat gcagcagcgc gccgagctgc
      841 tgggcggtca gctgaccgcg gacagctcac ctggccgcgg aaccgtcgtg cggctgcgca
      901 tcccgattta acgggagcgc tgatttaaca gcagcactac cattcgtcgc gcatgttgac
      961 cgttcgtcgc attctgaggc ttgtttcctc gggctgtagt cgcgggacca gaaattgtcg
     1021 gaggtggtcg cgataatggt gccatgacca cggtggagga ggatcgcacc caggcccgca
     1081 ttgacgggtt gcttcaagtg ctgcgcgacg cagaatccga tttccggcgg tcctatgcgc
     1141 gggtccttga cgtggttgcc gaactggatg ccgaaagggc aggagcggtc accggattcg
     1201 gcactacagc ccggttacta gccggtgcac tcaacccgac gaaaggcgag gccgtggatg
     1261 gcgggagatg atcaccgact cgacggtcga gcggatccaa accctgctgc ggatagcgct
     1321 tttatctccg cagctgcgga ggtactcttg ccggcgtgcc gcactatcgg atgtcagaga
     1381 tcgctgaact gttcggcgtg agcgatgaca ccgttcggcg gtgggcggat tcggggcagc
     1441 tcactgcgat ccgggatgcc ggtggtcgca aggcggtaga tggtgcggaa ctggcggcgt
     1501 ttgctaaaag ccaggcgcac gccacgcccg atccgtcgtt ggtggatcgg tcggcccgta
     1561 accgttttgt cggcttggtc accgaggtga tctgcgacct ggtgatggca caggtggaga
     1621 tccagtgcgg accacatcgg gtggtctcac tgatgagcag cgaagcagta cgcgaactgg
     1681 ggctcgcgcc gggggtgctg gcagtggctg tcgtgaagtc gaccgaggtc atggtcgaga
     1741 ccccaggagg acgagcatga
//
LOCUS       DATADN010000724         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1153201, whole genome shotgun sequence.
ACCESSION   DATADN010000724 DATADN010000000
VERSION     DATADN010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153201"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..497
                     /locus_tag="VHH53_09420"
     CDS             96..497
                     /locus_tag="VHH53_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2290393.1"
                     /translation="MISVAAELAGMHAQTLRGYDRMGLVSPGRSPGGGRRYSVRDIAL
                     LQEVQRLSQDHGVNLAGIKRIIDLENQVEALRARVAELAAELDEARIELAHAQAAAEQ
                     AAAAVHASYRRDLVPVHPRTETALVVWRPRR"
     gene            complement(641..1813)
                     /locus_tag="VHH53_09425"
     CDS             complement(641..1813)
                     /locus_tag="VHH53_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2290394.1"
                     /translation="MTADAVLGTALGPGRAPYGAGATMVRLIRESYAVIHPRADEVVR
                     FFYATLFAIAPATRELFGINMEGQQGRLLRALMYIVQKVDQPEELLPFLRQLGRDHRK
                     FGVLTPHYEAMGTALILALKQYSGPAWNAEVEKAWTDAYSLIARTMLEAADEDRDPAW
                     WTAQVIDHQRLTWDLAVVRVQPDRPVPYRAGQYVSVEVPQRPRLWRYLSPANAPRPDG
                     AIEFHVRAVPHGWVSRSIVGHTQVGDTWRIGSPMGRMTVDRHCGRDVLMVAGGTGVAP
                     MRAILEELSSWGTNPGVTMFVGGRIREDLHDLDTLVAMTITNPWLRVIPVLESRPREA
                     GIEHGTLADVVTRFGAWADRDVMVCGSPAMIRATVSRMLVAGTPLDRIFYDPFTVD"
     gene            2029..>3036
                     /locus_tag="VHH53_09430"
     CDS             2029..>3036
                     /locus_tag="VHH53_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2290395.1"
                     /translation="MDSFNPTTKTQAAISSAAQAASMAGNPDVTPAHLLGALLAQADG
                     IAAPLLAAVGADATAVRAELDVITKRLPSATGSTVAAPQLDRQAAKALGHAQNLATDM
                     GDSYVSTEHLLVGLAADAGPVGQLLRSHSATPDALKEAFQAVRGSARVSSPDPEGTYQ
                     ALAKYGLDLTDRARSGELDPVIGRDAEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVAGDVPESLRNKRVVALDLSSMVAGAKYRGEFEERLKAVLKEITDSAGQIIT
                     FIDELHTIVGAGATGESAMDAGNMIKPMLARGELRMVGATTLDEYRKHIERDAALERR
                     FQ"
BASE COUNT          525 a         1043 c         1014 g          454 t
ORIGIN      
        1 gacccgcggg ccaacctgtt cgcccgggca cacagccagg gagcggcgcg gtgatcccaa
       61 ccccaccggg cgcaaaccag gacaccccga ttttcgtgat ctctgtcgcc gcggagctgg
      121 cagggatgca cgcacagaca ctgcgcggct atgaccggat ggggctggtg tcgcccggcc
      181 gtagcccagg cggtggccgc cggtattcgg tgcgcgatat cgcgctgctg caggaggtgc
      241 agcggctgtc ccaggaccat ggcgtcaacc tggccgggat caagcggatt atcgatctgg
      301 aaaaccaggt ggaggcgctg cgcgccaggg tggctgagct cgctgccgaa ctggacgagg
      361 cgcgtattga actggcccac gcgcaggccg ctgccgagca ggccgccgcg gcggtgcacg
      421 cctcctaccg ccgtgacctg gtgccggtgc acccccgcac cgaaacagca ctagttgtct
      481 ggcgcccccg taggtagccc ttcaagtacc gccaacccgg tcaagactgc tctcaacagc
      541 taccaacatc tgctctcgac cagcggatcg agctgacagg ttggtagctg tttaaggtga
      601 tcaacacggg ggttggtggt acttgaagag cgcacaacta ctagtcgacg gtgaacggat
      661 catagaagat ccgatccagc ggggtgccgg cgacgagcat ccgggacacc gtggctctga
      721 tcatcgccgg ggagccgcag accatcacgt cccggtcggc ccaggcacca aagcgggtga
      781 ccacatcagc gagcgtcccg tgctcgatcc cggcttccct cgggcgcgac tccagcaccg
      841 ggatcaccct cagccacgga ttcgtgatgg tcatcgcgac gagcgtgtcg aggtcatgga
      901 ggtcttcgcg gatacggccg ccgacaaaca tcgtcacgcc cgggttggta ccccaactgc
      961 tgagttcctc gaggatggcg cgcatggggg ccactccggt gcctcccgcg accatcagca
     1021 cgtcccggcc gcagtgccga tccaccgtca tgcgtcccat cggcgaaccg atccgccagg
     1081 tgtcgccgac ctgggtatgg ccgacgatcg accggctgac ccagccgtgc ggcaccgcac
     1141 ggacgtggaa ctcgatcgcc ccgtccgggc gcggggcgtt cgccggagac aggtaacgcc
     1201 acagccgcgg ccgctgcggt acctccacgc tgacgtactg cccggcccgg tacggcaccg
     1261 gccgatcggg ctgcacccgt accacagcca gatcccaggt cagacgctga tggtcgatga
     1321 cctgcgcggt ccaccatgcg gggtcgcggt cttcatcggc ggcttccagc atcgtcctgg
     1381 cgatcaggct gtaggcgtcc gtccacgcct tttccacctc ggcgttccac gccgggcctg
     1441 agtactgctt caaggcgagg atcagcgcgg tgcccatcgc ttcgtagtgc ggcgtcagca
     1501 cgccgaactt gcggtggtcg cggcccagct gacgcaggaa cggcagcagc tcctcgggct
     1561 gatcgacctt ctgcacgata tacatcagcg cccgcagcag ccgcccctgc tgcccctcca
     1621 tgttgatacc gaacagctcg cgagtggccg gagcgatcgc gaacagcgtg gcgtagaaga
     1681 agcggacgac ctcatcggcc cgcgggtgga tcaccgcgta gctttcgcgg atcagccgaa
     1741 ccatcgtcgc gccggcgccg tagggcgcgc gaccgggtcc gagggctgtg cccagtacgg
     1801 catcggctgt catggtccgg gagcgtcttc tccaggtggg tgagcaggca gcggacccgc
     1861 cgcgggccct ggcgcagcag tatggcacgg cctgggtgca gtccggcgaa atggcgatga
     1921 cacgctgata ccggtgatcg ccaacgcata attgagcgga acacgctcaa ctttttcggc
     1981 gttgaagagt gcgacgggca agacgcaagt gcggaggacg cttccaggat ggattctttc
     2041 aatcccacca ccaagacgca ggccgccatc tcgtcggccg cgcaggccgc ctcgatggcc
     2101 ggcaacccgg atgtgacgcc ggcgcacctg ctcggggcgc tgctggctca ggccgatggc
     2161 atcgcggcac cgttgctggc ggcggtgggt gccgacgcca cagcggtgcg ggccgagctg
     2221 gacgtcatca ccaagcgcct gccgtcggcg accggctcca ccgtggccgc gccacagttg
     2281 gaccggcagg cggccaaggc gctcggtcac gctcagaacc tggccaccga catgggggac
     2341 tcctacgtct ccaccgagca cctgctggtg ggattggccg ctgatgccgg cccggtcggg
     2401 cagctgctgc gcagccacag cgccaccccc gacgcattga aggaagcgtt ccaggccgtc
     2461 cgaggttcgg cgcgggtgtc cagccccgac ccggaaggca cctaccaggc gctggccaag
     2521 tacggcctgg acctcaccga ccgggcccgc tccggtgagc tggacccggt gatcggccgc
     2581 gatgccgaga tccggcgggt ggtgcaggtg ctgtcccgac gcaccaagaa caacccggtg
     2641 ctcatcggcg aacccggcgt cggcaagacc gccatcgtcg agggactcgc ccagcgcatc
     2701 gtcgccggtg acgtgccgga gtcgctgcgc aacaagcggg tcgtcgcgct ggatctcagc
     2761 tcgatggtag ccggcgcgaa gtatcgcggc gagttcgagg agcggctcaa ggcggtcctc
     2821 aaggagatca ccgactcggc cggccagatc atcaccttca tcgacgagct gcacaccatc
     2881 gtcggcgccg gcgccaccgg ggagtccgca atggacgccg gcaacatgat caaacccatg
     2941 ctggcccgtg gcgaattaag gatggtcggc gccaccaccc tggacgagta ccgcaagcac
     3001 atcgagcgcg acgccgcgct ggagcggcgc ttccag
//
LOCUS       DATADN010000725         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1452809, whole genome shotgun sequence.
ACCESSION   DATADN010000725 DATADN010000000
VERSION     DATADN010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452809"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VHH53_09435"
     CDS             complement(<1..336)
                     /locus_tag="VHH53_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2290396.1"
                     /translation="MATLEIKDLHVEVRTDDEQTPDKEILRGVDLTVRAGETHAMMGP
                     NGSGKSTLAYSIAGHPRYQVTSGQVLLDGADVLAMSVDERARAGMFLAMQYPVEVPGV
                     SMTNFLRTAA"
     gene            complement(357..680)
                     /locus_tag="VHH53_09440"
     CDS             complement(357..680)
                     /locus_tag="VHH53_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX2290397.1"
                     /translation="MTAMRVCSLNDLKDDLPAGFEVDGVPVVLVRQGEQVYALADVCS
                     HAEIALSEGEVTRKGLECWLHGSCFDLRTGEPSSPPASEPVDTYAVRIDGEDVYVDPT
                     NPTNR"
     gene            complement(677..1870)
                     /gene="sufD"
                     /locus_tag="VHH53_09445"
     CDS             complement(677..1870)
                     /gene="sufD"
                     /locus_tag="VHH53_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX2290398.1"
                     /translation="MTATTDRSAGQGLVAHTHGDDVPMLSRGQRFTSYDVDAFEVPTG
                     REEEWRFTPLRRLRGLHEGVQATGAAKVIVEPVDGVAVEEVDRQDPRLGQGGVPADRV
                     AAQAWSATERATVVTVASETRIEQPVMITVAGPGPEEVAYGHLQLRLQPFAEAVVVLD
                     YRGSGTYAENVELILADGARLTLVNVQDWADDAVHVAAQHIRLSRDAVLRAITVSLGG
                     DLVRQSPTVTFTEPGGDAELLGLYFADAGQHLEHRLLVDHAVAHCRSNVAYKGALQGS
                     PGQPDAHTVWIGDVLIQAAAEGTETFEVNRNLVLNDGARADSVPNLEIETGEIVGAGH
                     ASTTGRFDDEQLFYLTSRGIPAELARRLVVRGFFGEILQKITVPQVRERLEAAIEAEL
                     EAVGA"
     gene            complement(1872..>2585)
                     /gene="sufB"
                     /locus_tag="VHH53_09450"
     CDS             complement(1872..>2585)
                     /gene="sufB"
                     /locus_tag="VHH53_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX2290399.1"
                     /translation="EGCTAPIYKSDSLHSAVVEIIVKNGGRCRYTTIQNWSNNVYNLV
                     TKRATAAEGATMEWIDGNLGSKVTMKYPSVWLVGEHAKGEVLSVACAGEGQHQDAGAK
                     MVHAAPHTSSTIVSKSVARGGGRTSYRGLVQVNEGSHHSRSTVKCDALLVDQISRSDT
                     YPYVDIREDDVSMGHEATVSKISDDQLFYLMSRGLTEDAAMAMIVRGFIEPIARELPM
                     EYALELNRLIELQMEGAVG"
BASE COUNT          409 a          879 c          838 g          459 t
ORIGIN      
        1 ggccgcggtg cgcaaaaagt tcgtcatcga cacaccgggt acctcgaccg ggtattgcat
       61 cgccaggaac atgcccgcgc gggcacgttc gtcgaccgac atcgcgagca cgtcggcgcc
      121 gtcaagcagc acctgcccgg aggtgacctg gtatcgggga tgcccggcga tcgagtaggc
      181 cagggtggac ttgcccgacc cgttcgggcc catcatcgcg tgggtctccc ctgcccggac
      241 ggtcaggtcg acaccgcgca ggatctcctt gtcaggagtc tgttcgtcgt cggtacggac
      301 ctcgacgtgc agatccttga tctccagagt ggccatcggt gtgttctcag ctcctgctag
      361 cggttagttg ggtttgtggg atcgacgtat acgtcctcgc cgtcgatccg cacggcgtag
      421 gtgtccaccg gttcggaggc cgggggtgaa gacggctctc ccgtgcgcag gtcgaagcac
      481 gagccgtgca gccagcactc caggccctta cgggtgacct caccctcgga cagcgcgatc
      541 tcggcatgcg agcagacgtc ggccagtgcg tacacctgct cgccctggcg caccagcacc
      601 accgggacgc cgtcgacctc gaagccggcg ggcaggtcgt ccttgaggtc attgaggctg
      661 cagacccgca ttgcggtcat gcccccaccg cctcgagctc ggcttcgatc gccgcctcga
      721 gtcgctcgcg tacctgcggg acggtgatct tctgcaggat ctcgccgaag aaaccccgga
      781 caaccagccg gcgggccagc tcggctggga tacccctgga ggtcaggtag aagagctgct
      841 cgtcatcgaa gcggcccgtg gtgctggcgt gcccggcacc gacgatctct ccggtctcga
      901 tctccagatt gggcaccgag tcggctcgtg cgccgtcgtt gagtaccagg ttacggttga
      961 cctcgaaggt ctcggtgccc tcggcggcgg cctggatgag cacgtccccg atccacacgg
     1021 tatgtgcgtc tggctgtccg ggactaccct gcaacgcgcc cttgtaggcg acgttggacc
     1081 ggcaatgggc aacggcgtgg tcgaccagca accggtgttc caggtgctgc ccggcgtcag
     1141 cgaagtacag gccgagcagt tcggcgtcgc cgccgggctc ggtgaacgtc accgtcgggg
     1201 attgccggac gagatcgcca cccaggctta cggtgatcgc tcgcaggacc gcgtcgcggc
     1261 tcagccggat gtgctgtgcg gcgacgtgca ctgcgtcgtc agcccagtcc tgaacgttga
     1321 ccagggtcag acgggcaccg tcggcaagga tgagctcgac attctcggca tacgtgccgc
     1381 tgccgcggta gtcgagcacc accacggcct cggcgaaggg ctgcaggcgc agctgcaggt
     1441 gcccgtaggc gacctcctcc ggcccagggc cggcaaccgt gatcatcacc ggttgctcga
     1501 tgcgtgtttc cgacgccacg gtgacgacgg tggcccgctc cgtcgcactc caggcctgtg
     1561 cagcgacccg atccgccgga acaccgccct gtcccagccg cggatcctgg cggtccacct
     1621 cctcgacagc gaccccgtca accggctcca cgatgacttt cgcagcgccg gtggcttgga
     1681 caccctcgtg caggccccgc agccgccgca acggagtgaa gcgccactcc tcctcccgcc
     1741 cggtgggcac ctcgaaagcg tccacgtcgt aggaggtgaa ccgctgcccc cgagagagca
     1801 tcggcacgtc gtctccgtgc gtgtgcgcga ccagaccctg cccagcagat cggtcagtag
     1861 tagccgtcat ctcagccgac ggctccttcc atctgcagct cgatcaggcg gttgagttcc
     1921 aaggcgtact ccatcggcag ttcgcgggcg atcggttcga tgaacccccg cacgatcatg
     1981 gccatcgccg catcctcggt cagcccccgg ctcatcaggt agaacagctg atcatcgctg
     2041 atcttggaga cagtggcctc gtgccccatc gatacgtcat cctcgcggat gtcgacgtag
     2101 ggataggtgt ccgaccggct gatctggtcg accagcaggg cgtcgcactt caccgtggag
     2161 cgagagtggt gactgccctc gttgacctgc accagcccgc ggtaggaggt gcggccaccg
     2221 ccgcgggcca ccgacttgga cacgatcgtg ctcgaagtgt gcggtgcggc gtgcaccatc
     2281 ttcgccccgg cgtcttggtg ctggccctcg ccggcgcagg ccacggagag cacctcgccc
     2341 ttggcatgct cgccgaccaa ccagaccgac gggtacttca tggtgacctt ggagcccagg
     2401 ttgccgtcga tccactccat ggtcgcgcct tcggcggcgg tggcccgctt agtgaccagg
     2461 ttgtaaacgt tgttcgacca gttctggatc gtggtgtagc ggcagcggcc gccgttcttg
     2521 acgatgatct cgacgaccgc ggagtgcaac gagtcggact tgtagatcgg cgctgtacag
     2581 ccttc
//
LOCUS       DATADN010000726         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_74648, whole genome shotgun sequence.
ACCESSION   DATADN010000726 DATADN010000000
VERSION     DATADN010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74648"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..911
                     /locus_tag="VHH53_09455"
     CDS             3..911
                     /locus_tag="VHH53_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX2290400.1"
                     /translation="MLVGVAADAVFGDPRRYHPVAAFGQLAATAERACYAEHRLVGLV
                     YTGALVGGVAAVGGLLERAGRRRAGMQVAGTALATWIALGGASLAQEGAAMARALDAG
                     DLDAARARLPHLCGRAPDDLDLAGVARASVESVAENTSDAVVAPLVWGAVAGVPGLLA
                     YRAVNTLDAMVGYRSPRYRRFGWAAARLDDLVNLLPSRAAAGLATLCAPVVGGSARGA
                     WQAWRRDAPAHPSPNAGQVEAAFAGALEIRLGGRTVYPHGAEQRPVLGHGRTPDAGDL
                     TRGVELSRVIGLAAAALCAVLAGVAQ"
     gene            complement(905..1746)
                     /locus_tag="VHH53_09460"
                     /pseudo
     CDS             complement(905..1746)
                     /locus_tag="VHH53_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
     gene            complement(1743..2165)
                     /locus_tag="VHH53_09465"
                     /pseudo
     CDS             complement(1743..2165)
                     /locus_tag="VHH53_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
BASE COUNT          308 a          751 c          797 g          321 t
ORIGIN      
        1 gcttgctggt cggtgttgct gctgatgcgg tattcggcga tccgcggcgc tatcacccgg
       61 tggctgcctt cggccaactc gctgcgaccg ctgaacgcgc gtgctacgcc gagcaccggc
      121 ttgtcgggtt ggtgtacacc ggcgccctgg tcggtggcgt ggcggcggtc ggtggcctgc
      181 tggagcgagc cgggcgcaga cgggccggta tgcaggtggc gggcaccgcg ctggcgacct
      241 ggatcgcgct gggtggcgct tcgcttgccc aggaaggcgc tgcgatggcc cgggcgctgg
      301 acgcggggga cctcgacgcc gcgcgggcac ggctgccgca tctgtgtggc cgcgcgccgg
      361 acgatcttga tctggccggc gtggccagag ccagcgtgga atccgtcgcc gagaacactt
      421 ccgacgcggt cgtcgcgccg ctggtctggg gcgcggtggc tggggtgcca gggctgttgg
      481 cctaccgcgc ggtcaacacc ctggatgcga tggtcgggta ccggtcgccg cgttaccggc
      541 gattcggttg ggccgcggct cggctggacg acctggtcaa cctgctaccg tcccgggccg
      601 ctgccgggtt ggccacgctg tgcgcgccgg tggtgggagg ttcggcccgc ggcgcgtggc
      661 aggcctggcg gcgcgacgca ccagcgcatc ccagccccaa cgcggggcag gtcgaggcgg
      721 cgttcgcggg tgccctggag atacggctgg gagggcgcac cgtctacccg cacggggcag
      781 agcagcgtcc ggtcctgggc cacggccgca cgcctgacgc cggtgacctg actcggggag
      841 tcgagctgtc tcgggtcatc gggctggcgg ccgcggcact gtgcgcggtg cttgccggtg
      901 tggctcagta gccatactta ttggagccgt aacggtcggt gagcgcggcc atcggtgggt
      961 cacgcgggtc gtcggtatcc ggagaccact cctcgcgacg ccagccggtc gcttcacgcc
     1021 aggcgaagta ggccagcccc agcggggcca tcgcgccgaa cgcgagccac tgcagcgcgt
     1081 aagcgaaaga cggcccggca tacagctgcg gcagcggcag cgcggacagc accccggggg
     1141 ctgcctcagt tagttgtacg taacccggct cgagtcgcac cccggtggcc gcgctcacgg
     1201 tgcgggtatt caccgcgtag acctgccggt gaccgtcctg ggccacgact gcaccaccct
     1261 gcgggtccgg ctcgtcggct cgcaaccggc cggtcacggt tacctcgcca ccgggcaccg
     1321 ggggataggc gggcacctgc ccctcgaccg gacgtagata gccgcgatcc acgagcacga
     1381 tcaatccgtc cgtcaactca agcgggacca acacctcgta cgccggctcg ccctgcacgg
     1441 tgcgcagccg cacgaccgtc tcggcgttgg caggtagcgc ccggtgatga cgacctgccg
     1501 ccactcggtg ccctgcgctg gcgcaacgcc cgcggcgagt acctcacgta gcggctgcgg
     1561 tgggatgttg aaggagcgct cgatagcggc gttgcgctcg gcacgctgct cggcgcgccc
     1621 gaactgccac ggcgcaagca gggtgaatgc ggcgacggag aagctcacca ccaccgccgt
     1681 cagcgctatc cagccaggac gcagcagaaa ccgccaccgc accccaccac ggtaaccggt
     1741 gttcacctca gcgttgcgcg tgcagcggcg gctgcgctgg cgccatagct gccaccgaac
     1801 aggactgcat gcaccagcag cgggaaaagc tggtgcagcg ggacacgctg ttgccaaccg
     1861 gatgccagtg gggcgacatc ctggtaaccg gcgagcaact gctccaggtg cgagaggccg
     1921 aacagcgcga gcatcgccag gtcggtttcc cggtgcccgc cgtgcgcggc ggggtcgagc
     1981 agccatagct gcccgtcggc gccggcgtgc acgttgcccg cccacaggtc cccatgcaac
     2041 cgggccggtg actcggttgg tccggccagc tcgtcgatcc gctcacagac ctggtgcacc
     2101 gcacgggccc cggtggcgtc catggcgcgg cgatccacgg cactgctcaa gtagcacaac
     2161 acccgggtgt tgtgcta
//
LOCUS       DATADN010000727         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_546759, whole genome shotgun sequence.
ACCESSION   DATADN010000727 DATADN010000000
VERSION     DATADN010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546759"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VHH53_09470"
     CDS             <1..194
                     /locus_tag="VHH53_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290401.1"
                     /translation="KEAARLVAQAEGMFLDPVFGAKAMAALMSASGGVIDNQGVVGPV
                     VFLLTGGAPTLFTATGGTL"
     gene            191..>1518
                     /locus_tag="VHH53_09475"
     CDS             191..>1518
                     /locus_tag="VHH53_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX2290402.1"
                     /translation="MTGPAEELIDAGYALEIADAPLVHRGLTLADLAHVLDLRSAGVI
                     PQQPARQLLGLLLDTLDIPAEEFPYDPSHGEVYNSRERYFTARIGDAAGWLHAGRPRR
                     EAMRIAFRLHLRDVLADLVGTAADLATQLAGLAELHTETWMADQTYLQHAQPTTFGHY
                     AVSFAYPVLRDGRRLADALNWVDASPGGAGGVNGTALLTERSHVAELLGFGSVIEHTR
                     DAMWQTDGLVNALAASASLLVTQGKLAEDLEIWASHEFSYVTIADGYSRSSVLMPQKR
                     NPYALTMIRGASGVAIGRLSGLLALTKTPSARSDNLIFAYGEVPRALAAAVCTTRLSA
                     GVVRTLQVHGERLWSVLEGSFAQAADLAEHLMLAYRLDYRSAYRIVGHTVRTAADHGL
                     RGVDIDAAAVDAAALAVLGRSLGIDGGGVDGGALSRVLDPREVVRTRTARG"
BASE COUNT          213 a          528 c          527 g          250 t
ORIGIN      
        1 gcaaggaggc tgcccggctg gtcgcccagg cggagggcat gttcctcgac ccggtgttcg
       61 gtgcgaaagc gatggccgca ctgatgagcg cgtcgggtgg tgtcatcgac aaccagggtg
      121 tcgtgggtcc ggtcgtcttc ctgctcaccg gtggcgcccc gacgttgttc acggctaccg
      181 gaggcacgtt gtgaccggac ccgccgagga gctgatcgac gccgggtacg cgctggagat
      241 cgccgacgcg ccgctggtcc accgcggact gacgctggcc gatctcgccc atgtgctcga
      301 tctgcggtcg gctggagtga taccgcaaca gcctgcgcgt cagctgctcg ggctgctgct
      361 ggataccctc gacatccccg ccgaggagtt cccctacgac ccgtcgcacg gcgaggtgta
      421 caactcccgg gagcgctact tcactgcccg aataggcgat gccgcaggct ggttgcacgc
      481 cggtcgccct cgtcgcgagg ccatgcgcat cgccttccgg ctacacctgc gcgacgtgct
      541 cgccgacctc gtcgggaccg ctgccgatct tgcgacgcag ctcgccgggc tcgccgagct
      601 gcacaccgag acgtggatgg ccgatcaaac ctacctgcag catgcgcagc ccacgacctt
      661 cggccattac gcggtgtcct tcgcctaccc ggtgctgcgc gacggccggc gcctagctga
      721 cgcgctgaac tgggtggatg ccagcccggg cggtgcgggc ggggtgaacg ggacggcgct
      781 gctcaccgag cgcagccacg tcgccgaact gctcggtttc gggtcggtca tcgaacacac
      841 ccgtgacgcg atgtggcaga ccgacggcct cgtcaacgcc ctcgcggcga gcgcgagcct
      901 gctcgtgacg cagggcaagc tggccgagga cctggagatc tgggccagcc acgagttcag
      961 ctacgtcacg atcgccgacg gctacagccg ctccagcgtg ctcatgccgc agaagcgaaa
     1021 tccctacgcg ttgacgatga tccggggcgc gtccggcgtg gcgatcgggc ggctgtccgg
     1081 gcttcttgcc ctgaccaaga ccccgtcggc ccgcagcgac aacctgatct tcgcctacgg
     1141 cgaggtgccc cgagcgttgg ccgctgcggt gtgcaccacc cggctcagtg ccggcgtggt
     1201 tcggaccctg caggtccacg gtgaacgact gtggtcggtc ctggagggta gcttcgcgca
     1261 ggccgcggac ctcgccgagc acctcatgct ggcctaccgg ttggactacc gcagtgccta
     1321 ccgcatcgtg ggccataccg tgcgcaccgc agccgatcat gggctgcgcg gggttgacat
     1381 cgacgctgcc gcggtcgacg ctgcggcgct cgccgtgctc ggccggtcgc tgggtatcga
     1441 tggcggcggt gtcgacggtg gtgcgctgtc ccgggtgctg gatccgcggg aagtcgtccg
     1501 cacccgcacc gcccgtgg
//
LOCUS       DATADN010000728         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1284506, whole genome shotgun sequence.
ACCESSION   DATADN010000728 DATADN010000000
VERSION     DATADN010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284506"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..834)
                     /locus_tag="VHH53_09480"
     CDS             complement(19..834)
                     /locus_tag="VHH53_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2290403.1"
                     /translation="MGEQAGWQLGGNAPEAYERYIIPALFSGWAHDLVETAAIRAGER
                     VLDVACGTGVVARAAAPLVGETGQVVGVDVNEGMLAMARTVSPPPGAAIAWQQGDAAV
                     LPFPEASFDVVLCQQGLQYFPQRTAALQEMARVLVPGGRLALSVWRALERQPFFVALV
                     AALESHLGPEVAALQRAAFTLGDAEELRGLVTAAGFRTPRIRLVVQLMRARSLAEYLP
                     GYLAATPMAGAVAALEDARRSVLFQELQTALRPYVDDTGLAVPMECHVVVAQI"
     gene            1202..>2121
                     /locus_tag="VHH53_09485"
     CDS             1202..>2121
                     /locus_tag="VHH53_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2290404.1"
                     /translation="MRYRFGEWVLDTLCYELQRGGEAVPLRPKAFQVLAYLLGHRDRV
                     VSKQGLLEALWPGQFVEEATLNSCIMAVRKTLGDSGQTPRFVHTVRGRGFRFVAPAQE
                     EAPVLSGSRRWSGPPPAEARSGEERSATPAVETSATAAPSTAGEVKPITALCCAPVDL
                     PTLEASLGPEALYRLLQAWMALVHEVIQRYDGTITQGSSEGLTALFGAPAAQEDHARR
                     AILAALELQQRLRTHPTLHAPGPGGAFTVRIGVHSGPVVAGDLGVEPHRLYTAVGAPT
                     HLATRLQQHAVPGAILMSAATYELVRGEVHG"
BASE COUNT          341 a          745 c          707 g          328 t
ORIGIN      
        1 gtacccttgg aggcggtgct agatctgcgc cacgacgaca tgacactcca tcggcacggc
       61 cagcccggta tcgtcgacat acggccgaag cgcggtctgg agttcttgga acagcacgga
      121 gcgtctggcg tcttctaagg cggcgacggc ccccgccatc ggcgtggccg ctagatagcc
      181 ggggagatac tcggccagag agcgcgcgcg cattagctgc accaccagcc gaatacgtgg
      241 cgtgcggaac cccgccgccg tgacgagccc ccgtagctct tcggcgtccc caagcgtaaa
      301 ggctgcccgt tgcagggccg cgacctcggg acccaggtgc gactccagtg ccgccacgag
      361 cgccacgaaa aacggctgtc gttccagcgc gcgccagaca ctcagggcaa gccgcccacc
      421 cggcaccagt acccgtgcca tctcctggag ggcggcggtc cgctggggaa agtattggag
      481 cccctgctga catagcacga catcaaagct agcctcagga aacggcagga ccgcagcatc
      541 gccctgctgc cacgcaatag cggcgcccgg cgggggcgac acggtccgcg ccatggcgag
      601 catgccctcg ttcacatcca ccccgacgac ctgtcccgtc tctcccacca gaggggccgc
      661 agcccgggcg accacccccg tgccacacgc gacgtccagg acgcgctcgc cggcccggat
      721 agcggcggtc tcgacgaggt catgcgccca cccgctaaac agcgcgggga tgatataccg
      781 ctcatacgcc tccggcgcgt tgccaccgag ttgccatccc gcttgttcac ccatcgtgtc
      841 cccctttcct gtcccctgcc atgcccgcgg acgtcagtcc gtgatggctt gcggggtggt
      901 gtcctcctgg ttcctgaccg cctcaccgcg tacaagcccc cttcccttgc ccgccgccct
      961 gcgccagcca ccatgtggcc gtgacagctc ggggcatgca cggtccgctc gggcgtccag
     1021 agtgacacgc ggcgcgacaa cgggcgtgtt gtaatatgga ccgtgcgcgg acgcgggaca
     1081 cgacagttgc gtggtaagac catggtatgt gcgtgacatt ttcgtgatat gctacgtgcc
     1141 catccctatc accgggggag aacaggacgc ccgacgccgg cgggcaaggg aggggagggt
     1201 catgcgctat cggtttgggg agtgggtcct ggacacgttg tgctatgaac tccagcgtgg
     1261 tggggaggcg gtaccgctcc gccccaaagc atttcaggtc ctggcgtatc tcctgggcca
     1321 tcgtgaccgc gtggtctcca aacaggggtt gctcgaggcc ctctggccgg ggcagtttgt
     1381 cgaggaggcc accctgaact cctgcatcat ggccgtgcgc aagacgctcg gggacagcgg
     1441 gcagacgccc cggttcgtgc acaccgtgcg gggtcgcggc tttcgctttg tggcaccagc
     1501 gcaggaggag gcgccggtgc tctcggggtc ccggcggtgg tcgggcccgc ccccggcgga
     1561 ggcgcggtcg ggagaggagc ggagtgccac ccctgcagtg gagacatccg ccactgctgc
     1621 cccaagcaca gccggcgaag tcaaaccgat caccgccctg tgctgtgccc cagtcgacct
     1681 cccaaccctg gaggcctccc tggggccaga ggccttgtat cggctgttac aggcgtggat
     1741 ggcgctggtg cacgaggtga tccagcgcta tgacggcacc atcacccagg ggtccagtga
     1801 ggggttgacc gccctatttg gcgccccagc ggcacaggag gatcatgccc ggcgggcgat
     1861 cctggcggcg cttgagctgc agcagcgcct gcgcacgcac cccacactgc acgcccccgg
     1921 gcccgggggc gcgtttaccg tccgtatagg ggtgcactca gggccggtgg tcgccggcga
     1981 cctcggggtc gagccgcacc ggctctacac ggcagttggc gccccgaccc acctggccac
     2041 gcgcctccaa cagcacgccg tccccggggc catcctcatg agcgccgcga cctatgagct
     2101 ggtgcgtggg gaggtgcacg g
//
LOCUS       DATADN010000729         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_75094, whole genome shotgun sequence.
ACCESSION   DATADN010000729 DATADN010000000
VERSION     DATADN010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75094"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..625
                     /locus_tag="VHH53_09490"
     CDS             248..625
                     /locus_tag="VHH53_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2290405.1"
                     /translation="MSDDGHDDRVRAWVEGYIQAWNSNDPAVIGSLFTEDAAYYSEPH
                     SQPWRGRDEIVRQWLNRKDEPGETEFRWHPLTVSRELAVVQGTTVYRHPPRTYSNLWV
                     IRLDAEGRCTEFTEWWMQHPDSG"
     gene            1283..1426
                     /locus_tag="VHH53_09495"
     CDS             1283..1426
                     /locus_tag="VHH53_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2290406.1"
                     /translation="MRRGSPIRGKAAPRPPRYLRAAEVAASLQVSPKTVSRWATEGKL
                     PFL"
     gene            complement(1522..1980)
                     /locus_tag="VHH53_09500"
     CDS             complement(1522..1980)
                     /locus_tag="VHH53_09500"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX2290407.1"
                     /translation="MPIYLSLDQIQGDVTAEGYVDQIELMSFSHGLSHPQSTTDGVSG
                     KLSVAEIAVTKTTDRASVRLIEAAVLGKVFDKAQIAFVSTDRDMLVAYLVVDLSNTRV
                     SSAAISSGGDRPVEQLSLACEQIRWAFVEPGAREAVTFGWDLLNQKALPS"
BASE COUNT          407 a          833 c          808 g          402 t
ORIGIN      
        1 gtttcgcggc cctccccttc cagggcgagg atggcgtcgg cctgctcggg cccgatgacc
       61 ccggcctcga ccccccgcgc cacccgccgg tccagctcct ccaccgtcca cctcccgagt
      121 cgtccaccca agggtcgccc ctcccccggt ccgggaccag agcggacgcc cccagcttcc
      181 gggtcccgac cacccggttt ccctgggtag ttccggcacc ggataccatc cgcggcatgg
      241 tgagcggatg agcgacgacg ggcatgacga ccgcgtgcgg gcctgggtcg agggatacat
      301 ccaggcctgg aactcgaacg acccggccgt gatcggcagc ttgttcaccg aggacgcggc
      361 ctactacagc gagccgcaca gccaaccgtg gcgtgggcga gacgagatcg tccggcagtg
      421 gctgaaccgc aaggacgagc cgggcgagac ggagttccgc tggcatccgc tcaccgtgag
      481 ccgggagctt gccgtcgtgc aggggacaac cgtctaccgc cacccgccgc gcacctacag
      541 caacctgtgg gtcatccgac tggacgccga gggtcgctgc accgagttca ccgagtggtg
      601 gatgcagcac cccgactccg gatagctgtc ggaaccgcgg gaccacggca ccggtcccaa
      661 gaatgcctac ttggaggcgc ccgggttctg gtggggcccc cggtcttcaa actctgattt
      721 cgccgttcag cgtcgttcgt aggcgtctgc gacctgggca ctctcggcgc cacggctcgc
      781 catgaaccac tgtggtccgc cggtgttgct gtcgctgttg ctgtcggtgc cgtcctaggg
      841 tctgtctccg ttgcgagagg accctgacag catctacgtc tgagaacgct acgaccccga
      901 ggcgtccctg cccggggtcg tagcgccgga ccgggctgga ggtggaggca acaacccggt
      961 ccacggggcc gatggtcacc acggcaagtg ctcgagagca ccgaccaccg acctaccaag
     1021 gaacgaccgt ggcactggag ccgttacgat cgaacaagcc agcgccccgg tcgcgggtgg
     1081 accggtcggc cgggcgttcg cgcctcagac atgaacggcc ccctgggtct cccagccggg
     1141 gcgttcgtcg tgcgcagcgc tagaaagagc gcttgggctg tcagccgttt cgtcgcggcg
     1201 cgcgctgggg gaggcgctcc ttggcctcca gcagccgacg aaaccaccaa cagccagagc
     1261 atcggtccgc cgcacgagac ggatgcgccg tggcagccca atccgcggca aggccgcacc
     1321 ccggcctccc cgctacttgc gggccgcgga ggtggccgcc agcttgcagg tgtcgcccaa
     1381 gaccgtgtcc cgctgggcca ccgaaggcaa gctcccgttt ctgtagacgc ttggtgccca
     1441 ccgctgccac cagcggcaga gatccgccag ctggccgagg agctccaggt gcagccgatg
     1501 gcctagcctg tgatctcggt cctagctggg gagggctttc tgattgagca ggtcccagcc
     1561 gaaggtgacc gcctcgcgtg ctccaggttc gacgaaggcc cagcggatct gctcacaagc
     1621 cagcgacaac tgctccaccg gccggtcgcc gcccgacgat atggccgccg aagagactcg
     1681 ggtattgctc agatcgacca caaggtacgc gacgagcatg tctcggtcgg tgctgacgaa
     1741 tgcgatctgg gccttgtcaa acaccttccc caagacggct gcttcgatga gccgcaccga
     1801 ggcacggtcg gttgtcttgg tcacagcgat ctcggccacg gaaagcttgc cgctgacgcc
     1861 atcggtggtc gactggggat gcgacaggcc gtgcgagaag gacatgagct cgatctggtc
     1921 cacatacccc tcagcggtta cgtctccttg gatctggtcc aagctcaggt agatgggcat
     1981 gaccggttcc cctcctcatg ctggacgctt gggcctccag cgaccaatcc gactaagaag
     2041 ggaatctaac gatttgcggc atgtatgacc aggtgcctgc tgagcaaccc tgggcgagtc
     2101 ggtggtcgac gatctcgacg ctccaccctg accgcatgct tatcaggcac attcgcgggg
     2161 atgcgttcaa ccagctagag cgttcggcgg ccaggtcatc gccgacatgc cagtgaccgt
     2221 gggggaccgc tgccaaccga tgcctagggc atgtcggggg cacggttggc gagatgacgg
     2281 tgctcgaagc ctgtcggcga tggccaccag cttgccccga gggcgaggcc cgtcgccggt
     2341 gaccacttcc cttgttggca agggcggcag gcctgcgaag ctgtcgggtg aggggttcta
     2401 gcccggcctt atgctgcttg gccgtccgag gccaaggcgg tggaggctct
//
LOCUS       DATADN010000730         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_547235, whole genome shotgun sequence.
ACCESSION   DATADN010000730 DATADN010000000
VERSION     DATADN010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547235"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VHH53_09505"
     CDS             <1..288
                     /locus_tag="VHH53_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2290408.1"
                     /translation="TVTRHFRQHQQGKPTSTNPIASIFAWTGGLRHRGTLDATPEVTR
                     FAETLEKVCIDTVESGKMTKDLALLVGPDTPFLTTEDFLGELDRGLRQAMH"
     gene            322..630
                     /locus_tag="VHH53_09510"
     CDS             322..630
                     /locus_tag="VHH53_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX2290409.1"
                     /translation="MAGKPSKRWQWILGSAVVIGVIVLSSQDDGAGGGSGPCTMRVTA
                     DSLNVRSGPDSRDPVVETLVADAVVSANVTTRNGYRELGPDRWAAQLYLEPMPGSDCS
                     "
     gene            658..1695
                     /gene="trpS"
                     /locus_tag="VHH53_09515"
     CDS             658..1695
                     /gene="trpS"
                     /locus_tag="VHH53_09515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2290410.1"
                     /translation="MSSPVTDRVTAGRPRVLSGIQPTADSFHLGNYLGAIRQWVALQA
                     DHDAFYCVVDLHAITVEQDPQKLRHRTRVAAAQLFALGVDPDRSTVFVQSHVPEHTQL
                     SWVLECLTGFGEASRMTQFKDKSAKQGTDRVRVGLFTYPILQAADILIYQADQVPVGE
                     DQRQHIELTRDLAQRFNSRYGSTFTVPVAHIVKGAEKIYDLAEPTAKMSKSTSSPAGV
                     IDLLDDPKVSARKIRAAVTDAGREIHYDPFAKAGVSNLLVIYSAMSGRTIDDLVAAYA
                     GQGYGALKSDVAEALADFVTPLRQQVAGYIDDPAELDKLLARGAERAREVAGQTVRAV
                     YDRVGFLPAGG"
BASE COUNT          295 a          575 c          600 g          281 t
ORIGIN      
        1 accgtcaccc gccacttccg ccagcatcag cagggaaagc caacgtcgac gaacccgatc
       61 gcatcgatct tcgcgtggac cgggggtctt cgtcaccggg gcacgctgga cgcaaccccg
      121 gaggtcaccc gtttcgctga gaccttggag aaggtctgca tcgacactgt cgagagcggc
      181 aagatgacca aggacctggc gctgctggtg ggtccggaca ccccgttcct gaccactgag
      241 gacttcctcg gcgagcttga ccggggattg aggcaggcca tgcactgaca gatcactgga
      301 cggacggcgg ggagtggacc ggtggctggg aagccttcga agcggtggca gtggattctc
      361 gggtcggccg tggtcatagg tgtgatcgtg ctgagctccc aggatgatgg tgcgggcggc
      421 gggtcggggc cgtgcacgat gagggtcacc gcggacagcc tcaacgtgcg gtccggtccg
      481 gacagcaggg atcccgtcgt ggagaccctg gtcgccgacg cggtggtttc cgccaacgtg
      541 accacccgca acggttaccg ggagctgggg cctgaccgct gggccgcgca gctgtacctg
      601 gagccgatgc cgggcagcga ctgcagctga tcgcgcggcg gctgcaacaa tcgcggtatg
      661 tcgtctcctg tcaccgaccg tgtcaccgcc gggcgtcccc gggtgctgtc cgggatccag
      721 cccactgcgg actcgttcca cctcggcaac tacctcggtg cgatccggca gtgggtggcc
      781 ctgcaggccg accacgacgc gttttactgc gtggtcgacc tgcatgcgat caccgtcgag
      841 caggacccgc agaagctgcg ccaccggacc cgggtcgcgg cggcgcagct gttcgcactc
      901 ggcgtcgacc cagatcgctc cacggtgttc gtgcagagcc atgtgcccga gcacacccag
      961 ctgtcctggg tactggaatg cctcaccggc ttcggcgagg cctcccggat gacgcagttc
     1021 aaggacaagt ctgctaagca aggcaccgac cgggtcaggg tcgggctgtt tacctacccg
     1081 atcctgcaag ctgcggacat cctgatctac caggccgacc aggttccggt gggggaggac
     1141 cagcgccagc acatcgagct cacccgcgac ctcgcccagc gcttcaactc ccgctacggc
     1201 tcgacgttca ccgtgcccgt agctcacatc gtcaagggcg cggagaagat ttatgacctg
     1261 gccgagccga cggcgaagat gagcaagtcg acgtcgtcgc cggccggggt gatcgacctg
     1321 ctcgacgacc cgaaggtgtc cgcccgcaag atccgcgcag cggtcaccga cgccggacgg
     1381 gagattcact acgacccgtt cgccaaggcc ggcgtgtcga acctgctggt gatctactcg
     1441 gcgatgtccg ggcgcaccat cgacgacctg gtggcagctt atgcaggcca ggggtacggg
     1501 gcgctgaagt ccgacgtcgc cgaggcgctc gccgacttcg tcacgccgct gcgccagcag
     1561 gtggccggct acatcgatga cccagccgag ctggacaagt tactggcccg aggtgccgag
     1621 cgggcccgtg aggtcgctgg ccagaccgtg cgggcggtat acgaccgggt gggcttcctg
     1681 ccggcggggg gctgaacagg gaacctctga ggtccagggt ttaccccgat gccggtccgc
     1741 cctgacaggt g
//
LOCUS       DATADN010000731         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_833881, whole genome shotgun sequence.
ACCESSION   DATADN010000731 DATADN010000000
VERSION     DATADN010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833881"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VHH53_09520"
     CDS             <1..339
                     /locus_tag="VHH53_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042799817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2290411.1"
                     /translation="AIGRPLPGNRNDCKAWELSGAKDAVGKTTVIADGGYRGTGLVIP
                     HRHERGQAELPAWKEDHNASHRKVRARVEHAFARMKTWKVLRDCRLKGDGVHHAMLGI
                     ARLHNLTLAG"
     gene            448..1728
                     /locus_tag="VHH53_09525"
     CDS             448..1728
                     /locus_tag="VHH53_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2290412.1"
                     /translation="MDAHEVNRMRALLALFVADVFASVSRKDQRAKGDCYLRGLMLDG
                     RRKSIQAMAARLPDGNEQNLQQFVNQSTWDPVPVQRRLVERMLPLIEPTAWVIDDVSV
                     PKDGRMSVAVAPQYCGALGKRANCQVAVSVHAATDTASCPLQWRLFLPKEWASDTGRR
                     DLTRIPPEVTHREKWRLALGMLDTLADWGMTPPVVVADAAYGTNAHLRTALADRQLAY
                     VLAVRSDVSAHPFEAEPVAPARNGPVGCWPQPRYRATARSAAALAAGLGPEAFTPLTW
                     RHGSRGQLRSRFAAVRVRPAGKAVERPIKAAASAEQGWWDGVLPDCWLLTEWPLDAQA
                     PTGYWLSNLPADTPIADLVRLAKVRWRIEHDYRELKHGLGLDHFEGRSWPGWHHHVTL
                     VTAAHAFLTEQRLAPKAPGPASPSTKSLTPSRAS"
BASE COUNT          370 a          746 c          674 g          305 t
ORIGIN      
        1 gcaatcggac ggccgctgcc cggcaaccgc aacgactgca aggcatggga actgtccggc
       61 gccaaggacg ccgtcggcaa gaccacagtg atcgcggacg gcggctaccg gggcaccggc
      121 ctggtcatcc cgcaccgtca cgaacgcggc caggccgaac tcccggcctg gaaagaggat
      181 cacaatgcct cccaccgcaa agtccgcgct cgcgtcgagc acgccttcgc ccgcatgaag
      241 acatggaagg tcctgcgcga ctgccgtctc aagggagacg gcgtacacca cgccatgctc
      301 ggcatcgcac gtctgcacaa cctcaccctc gccgggtgac ggaaaacgag caggtcgcct
      361 gccatgctgt agatcattta cgggacaacg cttagtagga ctccgttagg tctttccgtt
      421 aggcctgatc acgagcatgt tgggtgtgtg gacgcacatg aagtgaaccg catgcgggcc
      481 ctattggcgt tgttcgtggc ggatgtattc gcctcggtgt cgcgcaagga ccagcgggcc
      541 aagggcgact gctatctgcg ggggctgatg ctggatggcc gtcgcaagtc cattcaggcc
      601 atggccgcgc ggctgccaga cggcaatgag cagaacctgc agcagttcgt gaaccagtcg
      661 acatgggatc cggtgcctgt ccagcggcgg ctcgttgagc gcatgctgcc gctgatcgaa
      721 ccgacggcct gggtgatcga cgatgtgtcg gtgcccaagg acggccggat gtcggtggca
      781 gtggccccgc agtactgcgg ggccctgggc aaacgagcca actgccaggt cgcggtcagc
      841 gtccacgccg cgaccgatac cgcttcctgc ccgctgcaat ggcggctgtt tctgcccaag
      901 gagtgggcgt cggacaccgg ccgccgcgac ctcacccgca ttcctcccga ggtcacacac
      961 cgcgagaagt ggcgccttgc gctgggcatg ctcgacaccc tcgccgactg gggcatgacg
     1021 ccaccggtcg tggtggccga tgccgcttat ggaaccaacg cacatctgcg gaccgccctg
     1081 gccgaccgac aactcgccta cgtcctggcc gtccggtcgg acgtgagcgc ccatcctttc
     1141 gaggcagagc ccgtggcccc ggcccgcaac ggaccggtcg gctgctggcc ccagccccgc
     1201 taccgtgcga ccgcgcggtc ggcagcagct cttgccgccg gcctcggacc ggaggcgttc
     1261 acccctctca cctggcggca cgggtcgaga gggcagctac gatcccgctt cgccgcggta
     1321 cgcgtacggc ccgccggcaa agcggtcgag cgtccgatca aagccgccgc ctcggccgaa
     1381 caggggtggt gggacggagt cctccccgac tgctggctgc tgaccgagtg gcccctcgat
     1441 gcccaagcac caaccggcta ctggctgtcc aacctgccgg ccgacactcc gatcgccgac
     1501 ctggtgcgtc tggccaaggt ccgctggcgc atcgagcacg actaccgcga actcaagcac
     1561 ggcctgggcc tggaccactt cgagggccgc tcctggcccg gctggcacca ccacgtcacc
     1621 ctcgtgaccg ccgcccacgc cttcctcacc gaacagcgcc tggccccaaa agcgcccgga
     1681 ccggcctcac cctctaccaa gtccttgaca ccctccaggg cctcctgagg tgctggaccg
     1741 gcatatgcac cacctgccag caacccctga ccagcggtta ctcaagacca agatcgagac
     1801 agacctaacg gagtcctact aggccctgtc ggctccgggc cgcccgggtc gcgcgggtgc
     1861 gcaccgcgtc gtagacagaa gtcatgaacg aagacaagac gtcatggcac cccggggaaa
     1921 tcgtgcccgc gagcgggatc tacgaatgcg actgcggggg agagcaccac tggagcacgg
     1981 acgtgaaggg ccaccgcttc ccgccgccgc cgaacggctg ctcgggcgag tactggaggc
     2041 tgcggacgca ggcccacccg gaggacgacc gtccgggctg accggcgcat ggcgg
//
LOCUS       DATADN010000732         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_232234, whole genome shotgun sequence.
ACCESSION   DATADN010000732 DATADN010000000
VERSION     DATADN010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232234"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1423
                     /locus_tag="VHH53_09530"
     CDS             185..1423
                     /locus_tag="VHH53_09530"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEX2290413.1"
                     /translation="MTTAPLTDRLVVRSHLVPGGVDLLDLLPSPRGALSWVQAGEGLV
                     AWGEVARVCTEGPHRFARAQDWWQSFCAQLDTYDDVGLPGSGPVAFASLAFDDSPGSS
                     VLVVPRVVVGRRNGLTWVTEFETGTVTGPVVPVRRPRGLCYFDGELPVTAWRDAVARA
                     VCRMRDGSAPAKVVLAHDLLAVADEPLDPRFLLHGLAARYPSCWTFVVDQLVGATPEL
                     LLRRRGTEVLSRVLAGTTWPHDSTDPEALAAELLGSAKDRDEHCYAVDSLAQELRPFC
                     TELLVPTAPSVLQLRYVLHLATEVRGVLHPAGPPLLTLADALHPTAAVGGTPTPAAVS
                     AISELEAMDRGRYAGPVGWVDAAGDGELGIALRCAQVAGRFARLFAGCGIVAGSDPDS
                     EVAEAAAKLVPIRDALEGVD"
     gene            complement(1517..>1814)
                     /locus_tag="VHH53_09535"
     CDS             complement(1517..>1814)
                     /locus_tag="VHH53_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEX2290414.1"
                     /translation="RRPAGTEYWRTPPGDPGAANVLSGPVYTRGAMALQALRVEVGDQ
                     DFFAALRTWATERRGGNGTVQDFLAVIERVSNEQVDDVAQTWLFTPTRPPSPPS"
BASE COUNT          241 a          631 c          653 g          289 t
ORIGIN      
        1 cgccagcaca acgagcccgg tcagcacccc agccgcggca agcagcaccc acgacatccg
       61 tctgcgcctt cggccgaggt cgtgggtcct ggtcagcgca gcggtcatcc ggccaagagt
      121 agcccgtgtg gccctcgatc gacgcgcaag ccgcggcacc gatggcccct aatacgctgg
      181 cggcgtgacc accgctccgc tgaccgaccg gctggtcgtt cgctcgcatc tggtgccggg
      241 cggtgtcgac ctcctcgatc tgctgccgtc cccccgcggg gcactgtcct gggtgcaggc
      301 tggtgaggga ttggtggctt ggggcgaggt ggcccgggtg tgcaccgagg gcccacatcg
      361 cttcgcccgc gcgcaggact ggtggcagtc cttctgcgcg cagctggaca cctacgacga
      421 cgtaggtctg cccggcagcg gcccggtcgc cttcgccagt ctcgccttcg acgactcccc
      481 gggcagctcg gtgctggtgg tgccgcgggt cgtggtaggc aggcgaaacg gcctgacctg
      541 ggtcaccgag ttcgagaccg gcaccgtgac cggacccgtg gtaccggtgc gccgcccccg
      601 cggtctgtgc tacttcgacg gtgagctgcc ggtgaccgcg tggcgcgatg cggtggcccg
      661 cgcggtgtgc cggatgcgtg acgggtcggc gccggcgaaa gtggtactgg cgcatgacct
      721 actcgcggtc gccgacgagc cgctggatcc ccgattcctg ctgcacgggc tggccgccag
      781 atacccgtcc tgctggacgt tcgtggtcga ccagctggtg ggtgccaccc cggagttgct
      841 gctgcgccgg cgcggcaccg aggtgctctc gcgggtgctg gccgggacga cctggccgca
      901 tgacagcacc gaccccgagg cgctggccgc tgagctactc ggctcggcga aggaccgtga
      961 cgagcactgc tatgcggtgg actcgctggc ccaggaattg cggccgttct gcaccgaact
     1021 gctggtgccc accgcgccca gcgtgctgca gcttcgctat gtgctgcatc tggccactga
     1081 ggtgcgcgga gtgctgcacc ccgcgggccc gccgttgctc accttggccg acgcgctgca
     1141 ccccaccgcc gccgttggcg gcacccctac ccccgccgcg gtcagcgcga tcagcgagct
     1201 ggaggcgatg gaccgtggac gctacgccgg cccggtgggc tgggtcgacg ccgccgggga
     1261 cggcgaactg ggtatcgcac tgcggtgcgc tcaggtcgca ggccggttcg cgcggttgtt
     1321 cgccggctgc gggatcgtgg ccggctcgga ccccgactcc gaggtcgccg aggccgccgc
     1381 caagttggtt cccatccgcg acgcactcga aggagtcgac tgagctgcta gccccgacat
     1441 ggagacatac agggctatca gtgccgtagc ggctcgatat gtctccatgt ccggatcagc
     1501 agggtcgagg aggcggtcag ctgggtgggg agggtgggcg agtgggggtg aacagccagg
     1561 tttgggcgac gtcgtctacc tgctcgttgg agactcgctc gatgacggcc aggaagtcct
     1621 gcaccgtccc gttgccacca cggcgctcgg tggcccaggt gcgcagcgca gcaaagaagt
     1681 cctggtcccc gacctccact cgcagcgcct gcagcgccat cgcgccgcgc gtgtagacag
     1741 gtccgctgag cacattagcc gcgccgggat cacccggtgg ggtccgccag tactcggtac
     1801 cagccggccg gcgc
//
LOCUS       DATADN010000733         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_547617, whole genome shotgun sequence.
ACCESSION   DATADN010000733 DATADN010000000
VERSION     DATADN010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547617"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VHH53_09540"
     CDS             complement(<1..1346)
                     /locus_tag="VHH53_09540"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX2290415.1"
                     /translation="MNQAVIVQDPCGRIHLLNVAARRLFPELGVGDKLDNSGKSFVTG
                     SGGRRVSGRPQRLADGWQAWVVNEVSEAPAVSEAGTAEAAATSADHHDFMLAAGRELA
                     AGVAHDAAAAALVRMAVPVLGEQAAVLLPAPRARLSWWRFGHGEARPARGTSRAPAPR
                     TAPVLANALRGVTGDMTTVPADELVALTAALGPDVTEQRPVVATPLRGPQRTEGLLVV
                     VRPVATELIPPLAALAGPALAAGRQRRARAAALTQLQAPLLPLGPEALPELPGVQLGV
                     AHRAAPGELAVGGDFYLVRPITDDYSLCVLGDVCGKGAEALAESSRVQHSLAALMVVE
                     QEPARLLHLLNRALLAAGRTVFTTLVLGAMQPVATGLMLTLSAGGHPAPLVLRDDGHV
                     EEVAVPGTVVGILPDARFGRISLTLKPGETCLLYSDGVTEAHNVAGEEFGEGRLVTV"
BASE COUNT          251 a          568 c          510 g          218 t
ORIGIN      
        1 accgtcacca ggcggccctc gccaaattcc tcgccggcca cgttgtgcgc ctcggtgacg
       61 ccgtcgctgt agagcaggca ggtctcgcct ggtttcagcg tgagggagat ccggccaaaa
      121 cgggcgtccg gcagaattcc caccaccgtc ccgggtaccg cgacctcctc gacgtggccg
      181 tcgtcccgca acaccaacgg cgcgggatgg ccgccggcgc tcagcgtgag catcagcccc
      241 gtcgcaacgg gctgcatcgc gccgagcacc agcgtggtga agacggtgcg gccggccgcc
      301 agcagggcgc gattgagcag gtgcagcaac cgggccggct cctgctcgac gaccatcaat
      361 gcggccagcg agtgttgcac ccggctgctc tcggccaaag cctcggcgcc cttgccgcac
      421 acgtcaccga gaacgcacag gctgtaatcg tcggtgatgg gacggacgag gtagaagtct
      481 ccacccaccg ccagctcccc aggagcggca cgatgcgcta cgcccagttg cacgccaggc
      541 agttccggca acgcctcggg tcctagtggc aacagcggcg cctgcagctg agtcagagcg
      601 gcagctcgag ctcgccgctg ccgtccagca gcgagcgccg ggccggccag cgccgccagc
      661 ggtgggatca gctcagtcgc gaccggtcga accactacga gcaacccctc agtccgttgc
      721 gggccccgca atggcgtcgc caccaccggc cgctgctcgg tgacgtcagg gccgagcgcc
      781 gccgtcaatg ccaccagttc gtcggccggc accgtggtca tgtccccggt gactccgcgc
      841 agcgcattgg ccaggaccgg ggctgttcga ggggcgggcg cccggctggt accgcgggcc
      901 ggccgagcct cgccgtgccc gaaacgccac cacgacaacc tcgcccgcgg cgccggcagc
      961 agcacggcgg cctgctcgcc cagcaccggg acggccatcc ggaccagtgc cgctgctgcc
     1021 gcgtcatggg ccactcccgc tgcgagctcg cgtccagccg ccagcatgaa gtcgtggtgg
     1081 tcagccgatg tggcagcggc ctccgcagtg ccagcctcgg agaccgctgg cgcctcagag
     1141 acctcattga ccacccaggc ttgccaacca tcggcaagcc gctgcggccg tccggatacc
     1201 cggcgtccgc cggacccggt gacgaaggat ttgccactgt tgtccaattt atcgccgacg
     1261 cccagctcgg gaaacagccg ccgagccgcc acattcagca gatggatccg gccgcatggg
     1321 tcctgcacga tgacggcctg gttcatgcca tcgagtagcc gtggcaaccc tggtaccagc
     1381 agatcggcaa ccgggtgagg agatgcagtg gcatctgacg cggaccccgt tgccgatctg
     1441 ctggtgcccg ggctgccctg gctactcgat gccgtggacc aagcggtgat cgtgcaagac
     1501 cgatccggcc gtatccagtt gctcaacgtg gcggcccggc ggctgtt
//
LOCUS       DATADN010000734         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_694576, whole genome shotgun sequence.
ACCESSION   DATADN010000734 DATADN010000000
VERSION     DATADN010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694576"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VHH53_09545"
     CDS             complement(<1..1181)
                     /locus_tag="VHH53_09545"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2290416.1"
                     /translation="MGALRGRTSLKEPVMEVIYPRCCGLDLHKKRVVTCLIVPGPDGT
                     PRKEVRTFGTMTEDLLALADLLAQAGCTHVAMESTGVYWKPVYNLLEGRFELLLVNAR
                     HMKAVPGRKTDVKDCEWLADLLRHGLLTASFVPDRPQRELRELARYRTALVQERSAEI
                     NRLQKTLEGANIKLASVATDIMGTSGRQMLAALVEGTTDGAVLAQLAQGRLREKLPAL
                     EQALTGRMGVHQRFLLARQLAHISFLDEQIEQVSAEIAERLRPFQELIDRLDVIPGVG
                     RRIAEVVIAEIGTDLTRFPTAGHLASWAGMCPGNNESAGNRKSGTTRKGSPWLRMALI
                     EAAQAAARTRNTYLMAQYRRLAARRGRKKALVAVGHSILVIAYYLLTRRTDYQDLGGQ
                     Y"
     gene            1759..>2577
                     /locus_tag="VHH53_09550"
     CDS             1759..>2577
                     /locus_tag="VHH53_09550"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2290417.1"
                     /translation="MMDIIYERCCGLDIHKRSVVACLIVPGPAGTPHKEIRTFGTMTD
                     DLLALADWLGEHAVTHVAMESTGVYWQPIYNLLEGSFALVLVNARHIKAVPGRKTDVR
                     DCEWLADLLRHGLLRASFVPDRPQRELRELTRYRTSLIRERAAEVNRLQKTLEGANIK
                     LASVATDIMGASGRAMLAALLAGTTDARTLAELAKGRLREKLPELERALTGQMAAHQR
                     FLLAQQLAHIDFLEATIDRVGAEIGERLRPFEEAISRLDTIPGVGRRVAEVLLTE"
BASE COUNT          434 a          862 c          849 g          432 t
ORIGIN      
        1 aaatactggc cgcccaagtc ctggtaatcc gttcggcgcg tgagcaggta gtaggcgatg
       61 accagaatcg aatggccgac cgcaaccaac gccttctttc gcccgcgccg tgctgccagc
      121 cgccggtact gcgccatgag gtaggtgttg cgcgtccggg cggcggcctg ggccgcttcg
      181 atcagtgcca tccgcagcca cgggcttccc ttccgggttg tgccgctctt gcggttgccc
      241 gcgctctcgt tgttgcccgg acacatcccg gcccaactcg ccaagtgccc cgccgtcgga
      301 aagcgcgtga ggtcggtgcc aatctcagcg attacaacct cggcgatgcg ccggcccaca
      361 ccggggatca cgtcgagccg gtcgattagt tcctggaaag ggcgcagtcg ctccgcaatt
      421 tcggcgctca cctgctcgat ctgctcatcg aggaagctga tgtgcgccag ttgccgggcc
      481 aggaggaagc gctgatgcac tcccatccgt ccggtgagcg cctgctccag cgccggcagc
      541 ttctcccgca gccgcccttg ggccaactgc gccaggaccg ccccgtccgt cgtgccttcc
      601 accaatgccg ccagcatctg gcgcccggag gtgcccatga tgtcggtggc gacactcgcc
      661 agcttaatat tggcgccttc caaggtcttc tgcagccgat tgatctcggc gctccgctcc
      721 tggacgagtg cggtgcgata acgggccagc tcccgcagtt cccgctgcgg ccggtcgggc
      781 acgaagctgg cggtgaggag gccgtggcgg aggaggtcgg cgagccactc gcagtccttc
      841 acatcggtct tgcggccggg caccgccttc atgtgccgag catttacgag cagcagctca
      901 aaccgccctt ccaagaggtt atagaccggc ttccagtaga cgccggtgct ctccatcgcc
      961 acatgcgtgc agccggcctg ggcaagcaag tcggccaagg ccaacaagtc ttcggtcatc
     1021 gtgccgaacg tccgcacctc cttgcggggc gtcccatccg gccccggcac aatcaggcag
     1081 gtgaccaccc gcttcttgtg caggtccaga ccacagcagc gcggatagat gacctccatc
     1141 accggctcct tcagcgaagt acggccgcgc agggcgccca cctgcagaag cgtctggtgt
     1201 gcgtgcttcc ccggagggag cgacaatcag tggttcctcg gggcgccccg gtcctgctgt
     1261 tgcgcgggct cagagcacca ggctgagccg acctcaattc gcgcgaccgc ccggctccga
     1321 gcatacggcg cacgctgggc attttcattc cgcagtggtg cccgaggggg catgagtaac
     1381 tgttgcaaaa cgccagcgtg cggtccgaac agtgagtttg gaccgcacgc caggagccga
     1441 cttgaatctg gcaggaaccg ggaagcggcc cattctgacc gtggaatggt gtccccgggg
     1501 acttttgcaa cagttgctca tgccccggtg gggcaccacc gcacgatgaa aatgttcggt
     1561 gaccgcgcta tgctcggcgc cggacggccc gagcgtcgag gtcggctcag cctggtgccg
     1621 cgagcccgtt caacagttgg accccgggtg ccccgaggta ccaccgattg tcgctccctt
     1681 cggggaagca cgcagagcag acgctgcatg cgatgggcac cccgctcggt ccgtcccaga
     1741 ccgacccagg gagcggggat gatggacatc atctacgagc ggtgctgcgg gctggacatt
     1801 cataagcgat cggtcgtggc ctgtctgatc gtgccgggac ccgcagggac gccccacaag
     1861 gagattcgga cctttggcac catgaccgac gacctcctcg ccctcgccga ctggctgggc
     1921 gaacacgcgg tgacccatgt ggcgatggag agcaccggcg tctactggca gccgatctac
     1981 aacctactgg aaggcagctt cgcgttggtt ctggtcaacg cccggcacat caaagcggtg
     2041 ccggggcgca agaccgatgt gcgcgactgc gagtggctgg ccgacttgct ccggcacggc
     2101 ctgctccggg cgagcttcgt gcccgaccgg ccgcagcggg aactgcggga gctgacccgc
     2161 tatcggacct cgctgatccg cgaacgcgcc gccgaggtga accgcctgca gaagaccttg
     2221 gagggcgcca acatcaagct ggcgagcgtg gcgaccgaca tcatgggcgc ctcgggacgg
     2281 gcgatgctgg cggcgctgct ggcgggcacc accgacgcac ggacgctggc tgaactggcc
     2341 aaggggcggc tgcgcgagaa gctgcccgaa ttggaacgag cgcttaccgg acagatggca
     2401 gcccatcagc gcttcttgct ggcgcagcag ctggcgcaca tcgactttct ggaagcgacg
     2461 atcgatcgtg tgggtgccga gatcggggag cggctgcgcc ctttcgagga agcgatcagc
     2521 cggctagata cgatcccggg cgtcgggcgc cgcgtcgctg aggtgctact cacggag
//
LOCUS       DATADN010000735         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_694712, whole genome shotgun sequence.
ACCESSION   DATADN010000735 DATADN010000000
VERSION     DATADN010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694712"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VHH53_09555"
     CDS             complement(<1..244)
                     /locus_tag="VHH53_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2290418.1"
                     /translation="MIALERVNKTYSKQPKPAVVELTLDVPKGLIVMLIGPSGCGKTT
                     TLKMINRLIEPTSGRVLLDGEDVTQVNPDQLRRRIGY"
     gene            complement(294..998)
                     /locus_tag="VHH53_09560"
     CDS             complement(294..998)
                     /locus_tag="VHH53_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2290419.1"
                     /translation="MLLRIGLESYLHYLSAHSTEVIFYAQQHFLLVLYSVTIATGISL
                     VLALVLHTNQLTPASWSRKLKVSSQETSLLLASAALTIPSLALFGLLQPLLGLGTAPS
                     IVALTLYAIYPVLRNTVAGLSSVDPAVLEAARGVGMGPVRRMLQVQVPLAWPLIISGI
                     RVAVLITISIAVVAAIINGPGFGQPLQDGLARLGATDSFNQVVTGTLGCLVVAAGYEI
                     IFFVAQRFTIPRGIRV"
     gene            complement(1050..1493)
                     /locus_tag="VHH53_09565"
     CDS             complement(1050..1493)
                     /locus_tag="VHH53_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290420.1"
                     /translation="MTVRLLYLIFRQLVAWLGLLARSSRSKNAEILVLRHEVAVLRRQ
                     VRRPRLSWADRAVFAALTRLLSQAGRLHRIVTPGTILRWHRDLATQRGTQPHAAAPMA
                     APRYLSCANWCCSWRRKTGPGVLGESRANSPDSVTSLRPAPCGRS"
BASE COUNT          354 a          553 c          567 g          294 t
ORIGIN      
        1 catagccgat ccgcctgcgc agctgatccg ggttaacctg ggtgacatcc tcaccgtcca
       61 gcaggacgcg cccgctggtg ggctcaatga gccggtttat catcttcagc gtggtcgtct
      121 tgccgcatcc cgacgggcca atcagcatca cgataaggcc tttgggcacg tctaaggtca
      181 gctcaacgac ggcaggtttt ggctgtttgg agtaggtctt gttgacgcgc tcaagggcga
      241 tcatcgggcg ctcagcgctt aggcgagaga gaccggtcat ggactgggcg gtctcagaca
      301 cggatccccc tcgggatggt gaagcgctgg gccacgaaga aaatgatctc gtatccggcc
      361 gcgaccacaa gacatcccag cgtgccggtg acgacctggt tgaaggagtc ggtggcgccc
      421 aaccgggcca ggccatcctg caacggctga ccgaaacctg gaccattgat gatcgctgcc
      481 accacggcga tcgagatcgt gatcagcacg gcaacccgaa ttccggaaat gatcaggggc
      541 caggccagcg gtacctgcac ctgcagcatt cgacgcaccg gacccatccc gacgccgcgg
      601 gcagcctcca gcacagcggg atccaccgag gacagtccgg ctaccgtgtt ccgcagcact
      661 gggtaaatcg cgtacagggt cagtgccacg atgctcgggg cggttccgag ccccagcagc
      721 ggctgcagta ggccaaacag cgctagcgat gggatggtaa gcgcggccga ggcgagcagc
      781 agcgacgtct cctggctgct caccttcaat ttacgcgacc acgacgctgg ggtgagctgg
      841 ttggtgtgca gcaccagggc gagtaccagc gatatcccag tggctatggt taccgagtag
      901 agcaccagca gaaagtgctg ctgagcgtaa aagatcactt cagtggagtg cgccgacagg
      961 tagtggaggt aagactccaa gccgatccgc agcaacatgg cgtgaggtta gcccgtcgcg
     1021 gcgaggcgcg gggtccacac cagctcgttt caagatcgac cacacggagc tgggcgcaag
     1081 ctggtaaccg agtccggcga gttcgccctg gattcgccga gaaccccagg tccagttttc
     1141 cgacgccagc tgcagcacca gttggcgcag ctcaggtacc gtggagcggc catcggtgcg
     1201 gcggcgtgtg gctgggtccc gcgttgcgtg gccaggtccc ggtgccaccg caagatcgtg
     1261 ccgggggtga cgatccgatg caggcggcca gcttgggaca gcaaccgagt cagcgccgcg
     1321 aacaccgccc gatccgccca cgacagtcgc ggacgccgga cctgacgacg taacacggcg
     1381 acttcatggc gcagcaccag gatctccgca ttcttcgatc gcgagctacg ggccagcagc
     1441 cccagccagg ccacaagctg ccggaagatc agatagagca gtcgtaccgt cacggcgggc
     1501 gatcatgcct gatggccagt gttcagcgat gccgcaggtc accggcatgg atcacctatt
     1561 cggcacccac aggtggtacc cgtgacgccg aacgctgagc cggctcggcc ttgtactggc
     1621 gcactccaag aggcgcaaga atcattacat gctgtgactt tcaaacctgc atcactaccg
     1681 caaacgctgc cgcgggtgtg ccttgagggt gtccttcggg gtggcctacg aggaggttgc
     1741 gtcgtcttgc ccccagcagc ccttgaac
//
LOCUS       DATADN010000736         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1154593, whole genome shotgun sequence.
ACCESSION   DATADN010000736 DATADN010000000
VERSION     DATADN010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154593"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1660
                     /locus_tag="VHH53_09570"
     CDS             413..1660
                     /locus_tag="VHH53_09570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2290421.1"
                     /translation="MLAKRGITTAFPIQTATLPDALAGRDVLGRGRTGSGKTLAFAIP
                     TISTLDRSGLRRTPRHPRALVLVPTRELANQVADTFAPLAAALGQRITTVYGGVGQGG
                     QVAAQRGGIDVLIACPGRLEDLLDQGHVRLDAVEVTVLDEADHMADLGFLPAVRRLLD
                     RTPAGTQRLLFSATLDAAVDVLVRRYLTDPVVHSVDPSVAPVSTMAHHMFTVEHADKA
                     SVVRELASGQRRSLLFTRTKHGAKKLAKQLAAAGLPAVELHGNLSQNARARNLAAFSE
                     GSARVLVATDIAARGIHVDEVGLVVHVDPPAEYKGYLHRSGRTARAGAKGIVVTLSTP
                     DQSGEMRTLTRQAGIRPVTTRVRPGAEQITALTGPPAPLVASAAAAGASAPSKSRRDG
                     GVVGAPRRGPRRATRRRSTRRAA"
BASE COUNT          296 a          636 c          608 g          303 t
ORIGIN      
        1 aatcccactc gcttgctaac ggcttcggaa gtcgtcggga atctgtcatc tccaacacca
       61 tcctcagccc ggtcggccag cccctcacgt caagcaacga ttacccatgg catgacggtg
      121 gcaaacctgc tcagcgccca cttacggctg ttagcagggg ctggcgcagg gtgtcgatca
      181 cgtagggcat aatggactgc gatggttaca ccgatgctgg tgtgaccgag gtcgactgcg
      241 acgcgcctga caaagcgcac gagcttctcc gggtctggga ctgcggggtg gcacgcatca
      301 gccgccaccc atccctcaag gagaggactt cctccgcgtg cctgcgacac accccgcttc
      361 cgttcaaccg tgcagtttcg gcgacctcgg ggttaccgcg aaattgctga ctgtgcttgc
      421 caagcgcggc atcaccacgg cgttcccgat ccagactgcg acattgcctg acgcactcgc
      481 tggccgtgac gtgctcggcc gcggtcgaac cggcagtggc aagacgcttg ccttcgccat
      541 cccaacgatc agcacgttag accgttccgg gctgcgccgc actcctcgcc acccacgcgc
      601 gttggtgctg gtacctaccc gggaactggc caaccaagtc gccgacacct tcgccccgct
      661 ggctgccgcc ctcgggcagc ggatcaccac ggtctatggc ggcgttggcc agggtgggca
      721 ggtggccgcc cagcgtgggg gtatcgacgt cctcatcgcc tgcccaggtc ggttggagga
      781 tctgctcgac cagggccacg tgcgcctcga cgccgtcgag gtcacggtat tggatgaggc
      841 cgaccacatg gccgacctcg gattcttgcc tgcggtgcgc agattgcttg atcgcactcc
      901 tgcgggtacc cagcggctgc tgttctcggc aaccctggat gcagccgtgg acgttctggt
      961 acgccgctac ctgaccgatc cggtggtgca ctcggtcgac ccgtcggtgg cgccggtttc
     1021 gacgatggcg caccacatgt tcaccgtgga gcatgccgat aaggcctcgg tggtccgcga
     1081 gctcgcctcc ggccagcggc gcagcctgct gttcacgcgc accaagcacg gcgcgaagaa
     1141 gctcgccaag cagctggccg ccgccgggct gcccgccgtg gagctgcacg gtaacctctc
     1201 gcagaacgcg agggcacgca atcttgcggc gttcagcgag ggtagcgcgc gggtattggt
     1261 ggctacggac atcgccgcgc gcggcatcca cgtcgatgag gtcggcttgg tggtgcatgt
     1321 cgatccgcct gccgagtaca agggttacct gcaccgttcc gggcgcactg ccagggcagg
     1381 cgccaagggg atcgtggtca ccctgtccac cccggaccag tccggggaga tgcggacact
     1441 gacgcgccag gccggcatca ggccggtgac gacgcgggtg cgtcccggtg ccgaacagat
     1501 caccgcactc accggcccgc cggcgccact ggtggcctcg gcggctgcag ctggcgcgtc
     1561 ggcaccgtcc aagagccgcc gagacggtgg cgttgtcggg gctcctcgtc gcggaccacg
     1621 gcgagccacc cggcgccgct caacccggcg cgccgcctga ggaagctcaa tacctgggtg
     1681 tcagtcgccg cggagcaggg cggctagttc ggcgcggtcg gagatgccga gtttcgcgca
     1741 ggcacggtag aggtgaccct cgacggtgcg gaccgacacg acgagccagc ggtacgccca
     1801 gcggctcgcc gaacgccagc acctcgatga cgtcacattg cgc
//
LOCUS       DATADN010000737         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_232764, whole genome shotgun sequence.
ACCESSION   DATADN010000737 DATADN010000000
VERSION     DATADN010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232764"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1379
                     /gene="coxB"
                     /locus_tag="VHH53_09575"
     CDS             414..1379
                     /gene="coxB"
                     /locus_tag="VHH53_09575"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX2290422.1"
                     /translation="MSPNGSDRSPRRPMRRKLLQALTAGLVLATATGCSYNWEDFPRL
                     GMPTPTTEEAPRILSLWQGSWAAALAVGVLVWGLILWSAFFHRRSRTKVEVPPQTRYN
                     MPIEALYTVVPLIIVSVLFYFTARDESELLSLKKKPDVTVNVVGFQWSWCFNYVEPVA
                     GSTGDAKKSPELDAIPDRFKKDFPANAGGVYDCGTPGTRNPQTNNPGPTLWLPKGKTV
                     RFVLTSRDVIHSFWVVPFLMKQDVIPGHTNAFEVTPNKEGTFMGKCAELCGVDHSRML
                     FNVKVVSPERYEQHLKDLVDKQQTGYVPAGIAQTSHEKNRETNNL"
     gene            1436..>2438
                     /locus_tag="VHH53_09580"
     CDS             1436..>2438
                     /locus_tag="VHH53_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2290423.1"
                     /translation="MPVRRQNRGSVVVKWLTTTDHKTIGTLYLVTSFAFFVIGGVMAL
                     FMRAELARPGLQIMSNEQFNQAFTMHGTIMLLMFATPLFAGFANWIMPLQIGAPDVAF
                     PRLNMFSYWLFLFGGLIVLGSLLTPQGAADFGWTAYTPLSGGERTPQVGGDLWIMGLA
                     LSGFGTILGAVNFITTIICMRAPGMTMFRMPIFTWNVLLTSVLVLLAFPVLAAALLVL
                     EADRRFGAQVFNAENGGAVLWQHLFWFFGHPEVYILALPFFGVISEIIPVFARKPIFG
                     YTGLIGATIAIAGLSSVVWAHHMFATGAVLLPFFSFMTYLIAVPTGVKFFNWIGTMWK
                     "
BASE COUNT          407 a          848 c          771 g          412 t
ORIGIN      
        1 tcccgcgcgt ctgtacaggg agggacggcg ggcccgggtg ctgctggacg cggcgcggga
       61 tccgcccacc cttcgtcgag cgaggccagc agggcctgcc gggcaacagg atgtagcgga
      121 gcagcggaag cagcatcgaa gtaggccaca caccaacgct agaacgtcca gcaggggaag
      181 gggcctcagg ggcgcgggga acggcgcgaa ctgccacaaa caacccgcag ccgccaaaca
      241 agcgaaccac cccaccgagt aggcacccct ccccgcgaac cctcggagag cgtcggctag
      301 ggtttggtcc gcataaacat ccaaacccct gcccgacgca gggcggcgac cgacccgcga
      361 gaaggccagg ccgcagccgt acagcgcggg cgagactctc gggaaggcgc tacgtgagtc
      421 ccaacggctc cgaccgctcg ccgcggcgcc cgatgcggcg gaagctgctg caggcactga
      481 ccgcgggcct ggtcttggcg acagccaccg gttgctcgta caactgggaa gacttccccc
      541 gccttggtat gcccaccccg accacggaag aggctccgcg gatcctctcc ctgtggcagg
      601 ggtcctgggc ggctgcgctc gccgttggcg tgctggtgtg gggtctgatc ctgtggagtg
      661 ctttcttcca ccggcgcagc cgcaccaagg tcgaggttcc tccgcagacc cggtacaaca
      721 tgcccatcga ggcgctgtac accgtggtcc cgctcatcat cgtctcggtg ctgttctact
      781 tcaccgcccg tgacgagtcc gagctgctca gcctcaagaa gaagcccgac gtcacggtca
      841 acgtggtcgg cttccagtgg agctggtgct tcaactacgt cgagccggtc gccggttcca
      901 ccggggacgc caagaagtcc ccggagctgg acgcgatccc ggaccggttc aagaaggact
      961 tcccggccaa cgccggcggc gtctacgact gcggcacccc cggcacccgg aacccgcaga
     1021 ccaacaaccc gggtccgacg ctctggctgc ccaagggcaa gacggtccgc ttcgtcctca
     1081 cctcgcgtga cgtcatccac tccttctggg tggtgccgtt cctgatgaag caggacgtca
     1141 tcccgggcca caccaacgcc ttcgaggtga cccccaacaa ggagggcacc ttcatgggca
     1201 agtgcgccga gctctgcggc gtcgaccact ctcggatgct gttcaacgtg aaggtcgtct
     1261 cccccgagcg ctacgagcag cacctcaagg acctcgtgga caagcagcag accggttacg
     1321 ttcccgccgg catcgcgcag acgagccacg agaagaaccg ggagacgaac aacctgtgag
     1381 catcctcaac gaaccccagg gtgccgcggc agcagggtcc cactaccagg acgagctgcc
     1441 ggtcaggcgc cagaaccgcg gcagtgtcgt ggtcaagtgg ctgacgacga cggaccacaa
     1501 gacgatcggc acgctgtacc tggtgacgtc gttcgcgttc ttcgtcatcg gtggcgtgat
     1561 ggcgctgttc atgcgcgccg agctggcccg gccgggtctg cagatcatgt cgaacgagca
     1621 gttcaaccag gcgttcacga tgcacggcac gatcatgctg ctgatgttcg cgacgccgct
     1681 gttcgccgga ttcgcgaact ggatcatgcc gctgcagatc ggcgcgcccg atgtggcgtt
     1741 cccgcggctg aacatgttct cgtactggct gttcctcttc ggcggcctga tcgtgctcgg
     1801 cagcctgctc accccgcagg gcgccgcgga cttcggctgg accgcctaca ccccgctcag
     1861 cggcggtgag cgcactcccc aggtcggcgg ggacctgtgg atcatgggcc tggcgctgtc
     1921 cggtttcggc accatcctcg gtgccgtcaa cttcatcacc accatcatct gcatgcgcgc
     1981 gccaggcatg accatgttcc gcatgccgat cttcacctgg aacgtgctcc tcacctcggt
     2041 gctggtgctc ctggcgttcc cggtgctggc ggcggcgctg ctggtgctgg aggccgaccg
     2101 ccgcttcggg gcgcaggtct tcaacgcgga gaacgggggc gcggtcctgt ggcagcacct
     2161 gttctggttc ttcggccacc ccgaggtcta catcctggcg ctgccgttct tcggcgtgat
     2221 cagcgagatc attcccgtct tcgcccgcaa gccgatcttc ggctacacgg ggctgatcgg
     2281 cgccaccatc gccatcgccg gcctgtcgtc cgtcgtgtgg gcccaccaca tgttcgccac
     2341 cggggcggtc ctgctgccgt tcttctcctt catgacctat ctcatcgccg tgcccaccgg
     2401 tgtgaagttc ttcaactgga tcggcaccat gtggaagg
//
LOCUS       DATADN010000738         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1285769, whole genome shotgun sequence.
ACCESSION   DATADN010000738 DATADN010000000
VERSION     DATADN010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285769"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VHH53_09585"
     CDS             complement(<1..868)
                     /locus_tag="VHH53_09585"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2290424.1"
                     /translation="MPAQREPAPSTVLGCSLPAVVVNPSNVNDVGRLRTQITRVCREL
                     GWADPLWLETTVDDPGGGQAKAALAADADVVLACGGDGTVRQVAQILAGSGTAMGLLP
                     TGTANLLARNLAIPLDDLTRATRIALSGNTRPIDVGWVTFDQGEEHVFLVMAGMGFDA
                     AIMAGAHHDSKTQLGSLAYFVSGVRAGARALREQRVRIVLAVDGQPELHREVRTVVVG
                     NCGKLLAGLVLMPGAKIDDGLLDVVTIAPKSLKGWMGVTARVLSRRPSGHRIVQHWQG
                     STVTVIAEEPEQA"
     gene            complement(912..1808)
                     /locus_tag="VHH53_09590"
     CDS             complement(912..1808)
                     /locus_tag="VHH53_09590"
                     /inference="COORDINATES: protein motif:HMM:NF023007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2290425.1"
                     /translation="MPQAVVPADPPAAVLGPDAAHPLATSVAVVDGAVDWMSFRLMDT
                     LGAESPLRHSDGWTFCDQALSDPESFTRWRAAVAQRLAQQHGPVPEQTTAAHVLQWYL
                     GIPTYLGGMLFHSARRVPSLEPQQLAFRLDLAWVQEVGLRPGPFWCLPGDRDAGHPDA
                     TPVPDEAALAAVLRRQVVAHATRFLNVYGPLVRFGRRTLWAAVTDMLDVTLLIAGRSF
                     GSAQAGADDARLVLARREQPLTSASTTCEVTDEHGRTHWIRRRGWCCFSYALPGVEHP
                     CASCPRVSDAERVRILATLKPL"
     gene            2167..>4186
                     /locus_tag="VHH53_09595"
     CDS             2167..>4186
                     /locus_tag="VHH53_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX2290426.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGIALEGVRQQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNRVRQQVLQLLSGYQGKEPTESGSGRGEG
                     TPSSSLVLDQFGRNLTASAREGKLDPVIGRGKEIERVMQVLSRRTKNNPVLVGEPGVG
                     KTAVVEGLAQKIVKGEIPETLKDKQLYTLDLGSLVAGSRYRGDFEERLKKVLKEIRTR
                     GDIILFIDEVHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKHIEKDPA
                     LERRFQPIQVGEPPVDHTIEILKGLRDRYEAHHRVSITDSALVAAATLADRYINDRFL
                     PDKAIDLVDEAGARMRIRRMTAPPELREFDEKIAAVRRDKGSAIDAQDFEKAAKLRDS
                     EKQLLVKKSEREKQWKAGDLDVVAEVDEEQIAEVLANWTGIPVFKLTEEETTRLLRME
                     DELHKRIIGQHEAVKAVSKAMRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKALAEF
                     LFGDDDALIQIDMGEFHDRYTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLFDEV
                     EKAHPDIFNSLLQILEEGRLTDSQGRVVDFKNTVIIMTTNLGTRDISKG"
BASE COUNT          759 a         1366 c         1409 g          652 t
ORIGIN      
        1 gggcctgttc gggctcctcc gcaatgacgg taacggtgct gccttgccag tgctgcacta
       61 tccgatggcc actgggacgg cggctcagca cccgcgctgt cacgcccatc caccccttga
      121 ggcttttcgg cgcgatggtc accacgtcca gtaacccgtc gtcgatcttg gccccaggca
      181 tcagcaccag accggcgagc agtttgccgc agttgcccac cactaccgtc cggacctcac
      241 ggtgcagttc gggttggccg tccacggcga ggacgatccg gacccgctgc tcgcgcagcg
      301 cgcgtgctcc ggcgcgcacc ccggagacga agtacgccag cgagcccagc tgggtcttcg
      361 agtcgtggtg ggcgccggcc atgatcgccg cgtcgaagcc catcccggcc atcaccagga
      421 agacatgctc ctcgccctgg tcgaaggtga cccagccgac gtcgatcggg cgagtgttcc
      481 cggacagcgc gatcctggtg gcgcgcgtca ggtcgtccag cggtatggcg agattgcggg
      541 ccaacagatt ggcggtgccc gtgggcagca ggcccatcgc ggtgcccgat ccggcgagga
      601 tctgtgccac ctgacggacc gtgccgtccc cgccgcaggc gagcacgaca tcggcatcgg
      661 ctgccagcgc cgccttggcc tgacccccac ctggatcgtc tacggtggtc tccagccaca
      721 gcgggtcggc ccatcccagc tctcggcaca ccctggtgat ctgcgtgcgt agccgcccca
      781 cgtcgttgac gttgctcggg ttgactacca ctgcgggcag cgagcagccc agcaccgtgc
      841 tgggcgccgg ctctcgttgc gcaggcactg aatgaagata cggcctacag cgggtgcaca
      901 gtgggtttag ttcacagagg ttttagtgtg gccaggattc ggacccgttc ggcgtcactc
      961 acccgtgggc agctggcgca cggatgctcg acacctggca gcgcataaga gaaacagcac
     1021 cagccacgtc gacggatcca gtgtgtgcga ccgtgttcat cggttacttc acaggttgtc
     1081 gacgctgagg taagcggttg ctcgcgccgc gcgagcacga gccgggcgtc atccgcaccg
     1141 gcctgcgctg atccgaacga gcggcctgcg atcagcaggg tgacgtccag catgtcggtc
     1201 actgcggccc acaacgtgcg gcgcccgaag cgcaccagcg gaccgtagac attcaggaag
     1261 cgggtggcat gcgccaccac ctgacggcgt agtacggcgg ccagcgctgc ctcgtcaggt
     1321 accggtgtcg catcgggatg gccggcatcg cgatcgccag gcaggcacca gaacgggccg
     1381 gggcgcaggc ccacctcctg cacccacgca aggtcaagcc ggaaggcgag ctgctgcggc
     1441 tccagcgatg gcacccgccg ggccgagtgg aacagcatcc cgccgaggta ggtggggatg
     1501 cctagatacc actgcagcac atgcgcggcg gtggtctgct cgggcacggg gccgtgctgc
     1561 tgcgccagcc gttgcgcgac tgccgcgcgc caccgcgtga aggattccgg atcggatagg
     1621 gcctggtcac agaaggtcca cccatcactg tgacgtaaag ggctctcggc gccgagagta
     1681 tccatgagcc ggaaggacat ccagtccacc gcaccgtcca ccaccgccac tgacgtggcc
     1741 agtgggtgtg cagcgtcagg tccgagcacc gctgccggcg ggtcagcagg caccacggct
     1801 tgcggcacgg tctctcctct gcattactag agatgcccca gttcaatccc agctcaagct
     1861 ttagcggcct aggcgcccgg tacagggttg cctaacctaa tatcgggcaa acttagcacc
     1921 agggtcagcg tggaagcacg gtttgagggc cgggaccacc cgagagtggt ggtgcgttca
     1981 ccctgagcgg acgggcgggt ggtcccggaa tcttgaccag cgtcggcacg ttggagcggg
     2041 tgaccggcag aagttcgaac gcggcgttca tcgcccggca gctacagtga cagcacggcg
     2101 ggactcgtgc cgagcagggc tcaccagtag gggccgcgtc ccacggcgca gcagtcagga
     2161 gtgcacatgt ttgagaggtt caccgaccgc gcgaggcggg tggttgtgct ggcccaggaa
     2221 gaggccagga tgctcaacca caactacatc ggcaccgagc acatcctgct gggtctgatc
     2281 cacgagggag agggcgtcgc cgccaaggcc ctcgaatcgc tggggatcgc cctggagggg
     2341 gtgcgccagc aggtcgagga gatcatcggc cagggccagc aggcaccgtc gggtcacatc
     2401 ccgttcacgc cacgggccaa gaaggtgctg gagctgtcgc tgcgcgaggc gctgcagctc
     2461 gggcacaact acatcggcac cgagcacatc ctgcttgggc tcatccggga aggcgagggc
     2521 gtggccgcgc aggtgctggt caagctcggt gccgacctca accgggtgcg ccagcaggtg
     2581 ctgcagctgc tgtcgggtta ccagggtaaa gagcccaccg aatcgggctc aggccgtggc
     2641 gagggcaccc cgtcgtcgtc gctggtactc gaccagttcg gccgcaacct caccgcaagc
     2701 gcccgcgagg gcaagctcga cccggtgatc gggcgcggca aggagatcga gcgggtcatg
     2761 caggtcctgt cccggcgcac caagaacaac ccggtactgg tgggcgagcc cggcgtgggc
     2821 aagacggccg tggtcgaggg cctcgcgcag aagatcgtca agggcgagat cccggagacg
     2881 ctcaaggaca agcagctcta caccctcgac ctcggttcgc tggtcgccgg ctcccggtac
     2941 cgtggtgact tcgaggagcg cctgaagaag gtcctcaagg aaatccgcac ccgcggcgac
     3001 atcatcctgt tcatcgacga ggtgcacact ctcgtcgggg cgggtgctgc cgagggcgcg
     3061 atcgacgccg cgtcgatcct caagccgatg ctggcccgcg gtgagttgca gaccatcggt
     3121 gctaccacgc ttgatgaata ccgaaagcac atcgagaagg accccgcgct ggagcgccgg
     3181 ttccagccga tccaggtcgg cgagccgccc gtggatcaca ccattgagat cctcaagggc
     3241 ctgcgcgacc gttatgaggc acaccaccgc gtttccatca ccgacagcgc actggtcgcc
     3301 gccgcgaccc tggccgaccg gtacatcaac gaccggttcc tcccggacaa ggcgatcgac
     3361 ctggtcgacg aggcgggcgc ccggatgcgg atccgccgga tgaccgctcc gccggaactc
     3421 cgcgaatttg acgagaagat tgctgcggtg cgccgggaca aagggtcggc catcgatgcc
     3481 caggacttcg agaaagccgc gaagctgcgg gattccgaaa agcagctgct ggtgaagaag
     3541 tccgagcggg agaagcagtg gaaggctggt gacctcgacg tcgtcgccga ggtcgatgag
     3601 gagcagatcg ccgaggtgtt ggcgaactgg accggcatcc ccgtgttcaa gctcaccgag
     3661 gaggagacca ctcggctgct gcgcatggag gatgagctgc acaagcggat catcggccag
     3721 catgaggcgg tcaaggccgt ttccaaggcg atgcgccgta cccgtgcagg tctgaaggat
     3781 cccaagcggc cgtccgggtc gttcatcttc gccggcccct cgggtgtggg taagaccgag
     3841 ctgtccaagg cgctggcgga attcctgttc ggtgatgacg acgcgctcat ccagatcgac
     3901 atgggcgagt tccacgaccg ctacactgca tcccggcttt tcggcgcccc gcccggatac
     3961 gtgggttacg aggaaggcgg tcagctcacc gagaaggtgc gccgcaagcc gttctcggtc
     4021 gtgctcttcg acgaggtcga gaaggcccac cccgacatct tcaactcgct gttgcagatc
     4081 ctcgaggaag gtcgcctgac cgactcgcag ggccgggtgg tcgacttcaa gaacacggtg
     4141 atcatcatga ccaccaacct cggcactcgt gacatcagca agggcg
//
LOCUS       DATADN010000739         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_232897, whole genome shotgun sequence.
ACCESSION   DATADN010000739 DATADN010000000
VERSION     DATADN010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232897"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VHH53_09600"
     CDS             <1..293
                     /locus_tag="VHH53_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF732 domain-containing protein"
                     /protein_id="HEX2290427.1"
                     /translation="VAAGLAAAAFASAPLAAADPESDFLNALESQGVTWDGATPANMV
                     AAGQGVCQDWDSGATFAQEVASLEAHMSMEDAAFLIGAATAAFCPQHEAKIQ"
     gene            complement(347..>1717)
                     /locus_tag="VHH53_09605"
     CDS             complement(347..>1717)
                     /locus_tag="VHH53_09605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2290428.1"
                     /translation="GQMWQMFGDVDAVHGLYYLFMHAWFGVFPPTEFWSRAPSALAIG
                     GAAAGIVVLGKQFSSRTVGLAAGALCAVLPRATWAGIEARPYALSMMAAVWLTVLLVH
                     AVRRGTAWLWVAYGVALACSTLLDIYLVLLWVAHAALIVLFHRSRTALAGFGISSALS
                     ICAVAPFIAVVVGQVHQISWITPIGRRTVEDVAVQQYFDRNPVFALLAALVVVVAVVL
                     WLSKVRRLGGGDRQLIVLAVAWAAIPTAVIVLWSSVAHAIYTPRYLAFTAPAVALVLG
                     VCIGALASAPWLATAVVGVFVVASAPSYLFAQRVPYAKYGMDYSQVADLITARAAPGD
                     CLLVNDTVTFHPAPMRPLLAARPDAYRKLVDVSLWQRATDRRDVFDTNLIPEASVGPL
                     ERCKELWIITQADRALPAHEQGAPMAPGPQFGGTTTFAVTHDMGFWLLERWQFNLVQV
                     IKAARV"
BASE COUNT          359 a          595 c          532 g          231 t
ORIGIN      
        1 cggttgcggc cggcctggct gcagccgcat tcgccagcgc gccgctcgcg gcagctgatc
       61 ccgagagcga cttcctgaac gcactggaaa gccaaggcgt cacgtgggac ggggcgactc
      121 cggcgaacat ggtcgctgcc ggacagggcg tatgtcagga ctgggacagc ggggccacct
      181 tcgcgcagga agtcgcgtcg ctggaagcgc acatgagcat ggaggatgcg gccttcctga
      241 tcggcgcggc gaccgccgcc ttctgcccac agcacgaagc caagattcaa tagacgacga
      301 tcaaatcagg ggcaggcgac gttgatctgc ccctgacgcc gtgcgctcag acccgcgcgg
      361 ctttgatcac ctgcaccaga ttgaactgcc atcgctcgag tagccagaaa cccatgtcgt
      421 gggtcacggc gaatgttgtc gtgccaccga attgcggacc aggcgccatt ggcgcgccct
      481 gctcatgtgc cggcagcgcc ctgtcggcct gcgtgatgat ccacaactcc ttgcagcgct
      541 cgagaggacc cacggaggcc tcgggaatca ggttggtatc gaacacgtct cgccggtctg
      601 tcgcgcgttg ccagagactg acgtcgacga gcttgcgata ggcgtcgggc cgtgcggcca
      661 gcagcggacg catcggcgcc gggtggaacg tgaccgtgtc attgaccagt aagcagtcgc
      721 caggtgccgc ccgcgctgtg atcagatcgg ccacctggct gtagtccatg ccgtacttcg
      781 catatggaac acgttgagcg aaaaggtaac tcggcgccga cgccacaacg aagacgccga
      841 cgaccgccgt agccagccac ggcgctgaag ccagcgcacc gatgcagaca cccagcacca
      901 aggccaccgc aggggcagtg aacgcaaggt agcgcggcgt gtagatcgcg tgtgccacgc
      961 tggaccacaa cacgatgacg gcggtgggaa tcgccgccca cgccacagcc aacacgatca
     1021 actgccggtc accgccaccc aagcgtcgaa ccttgctgag ccacaacacc acagccacca
     1081 cgaccacgag cgcagccagc agcgcgaaca ccggattgcg gtcgaagtac tgttgcaccg
     1141 caacgtcttc gaccgtccgg cggccgatcg gtgtgatcca actgatctga tggacctgcc
     1201 ccacgaccac cgcgatgaac ggcgccaccg cgcagatgct caacgccgag gagataccga
     1261 agcccgcgag agccgttcgg ctgcggtgaa acagcacgat caaggccgca tgcgccaccc
     1321 acagcagcac caagtagatg tccagcagtg tcgagcaggc cagcgcgact ccgtacgcca
     1381 cccacagcca cgcggtgcca cggcgcaccg cgtgcactag caggaccgtc aaccacaccg
     1441 cggccatcat cgacagcgca tacggcctcg cttcgatccc ggcccatgtc gcgcggggca
     1501 acacggcgca gagcgcaccg gcggccaatc cgactgtgcg cgaggagaac tgcttgccca
     1561 acacgacgat ccccgccgcg gcacccccga tcgccagcgc gctgggtgca cgcgaccaga
     1621 actccgtcgg cggaaaaact ccgaaccagg catgcatgaa caggtagtag agcccgtgga
     1681 cggcgtcgac gtcgccgaac atctgccaca tctggcc
//
LOCUS       DATADN010000740         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1454706, whole genome shotgun sequence.
ACCESSION   DATADN010000740 DATADN010000000
VERSION     DATADN010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454706"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /gene="serS"
                     /locus_tag="VHH53_09610"
     CDS             <1..1128
                     /gene="serS"
                     /locus_tag="VHH53_09610"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX2290429.1"
                     /translation="RAEQKALGKQVGRARGAERDELLAHAKQLAADVKLAEAEQADAE
                     AEIESAHRAVSNLVVDGVPAGGEDDYVVLKHVGRPPELDFEPSDHLELGTALGLFDTE
                     RGAKVSGSRFYFLTGLGAQLQLGLLQMAASQAVASGFTLMIPPVLVRPEIMDGTGFLG
                     AHAAEVYRLRDDDLYLVGTSEVPLAGYHAEEIIDLSGGPLRYAGWSSCFRREAGSYGK
                     DTRGIIRVHQFDKVEMFVYCQPQCAETEHQRLLAWEEQMLSAIEVPYRVIDVAAGDLG
                     SSAARKFDCEAWLPSQRAYRELTSTSNCTTFQARRLGIRYRDPSDPAGRPQVAATLNG
                     TLATTRWIVAILENHQQPDGSVRVPQALRPALGGIELITAG"
     gene            1265..2077
                     /locus_tag="VHH53_09615"
     CDS             1265..2077
                     /locus_tag="VHH53_09615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2290430.1"
                     /translation="MRRPRLVASDVDGTLLDPTDRISERTRAAVHRVLAAGVPFVLVT
                     GRPPRWIPPIVEQLGHAGYAVCANGAVLYDATTDRVSYSTTLDPLQLRDAANVVATAL
                     PGAKLAVERPTGGATLNGFDDFLAEPDYTHPWPGADSAPAPRDVLLGQPAIKLLVRQP
                     DTSSDVMAAAVREILGADLRASLNVTFSTGHGLIEISAPGVTKATGLARLAGELGVVP
                     ADVLALGDMPNDLAMLQWAGCGVAMANAHPAVLDCADEITARNSEDGLALVL"
BASE COUNT          333 a          745 c          717 g          351 t
ORIGIN      
        1 cgtgccgagc agaaggcgct gggcaagcag gtcggccggg cacgtggtgc agaacgggac
       61 gagttgctcg cgcacgccaa gcagctcgct gccgacgtca aactcgccga agccgagcag
      121 gccgacgccg aggcggagat cgagtccgcg caccgcgcgg tgtccaatct ggttgtcgac
      181 ggggtccctg ccggcgggga ggacgactac gtcgtgctca agcacgtcgg gcggcccccc
      241 gagctggatt tcgagccgag cgatcatctg gagctgggca ccgctttggg tttgttcgat
      301 accgagcgtg gcgcgaaggt ctccgggtcg cggttctact tcctcaccgg gctcggcgcg
      361 cagcttcagc tcgggctcct gcagatggcc gcgtcgcagg cggtggcctc cggtttcacc
      421 ttgatgatcc cgccggtact ggtccgcccg gagatcatgg acggcaccgg cttcctcggc
      481 gcgcatgcgg ccgaggtgta ccggttgcgc gacgacgacc tctacctggt gggcacctcc
      541 gaggtgccgt tggccggtta ccacgccgag gagatcatcg acctgtccgg cgggccgttg
      601 cgttacgccg gctggtcatc ctgcttccgc cgcgaagccg gttcctacgg caaggacact
      661 cgcggcatta tccgggtgca ccagttcgac aaggtcgaga tgttcgtcta ctgccagccg
      721 cagtgcgctg agaccgaaca ccaacggttg ctggcctggg aggagcagat gctctccgca
      781 atcgaggtgc cctaccgggt gatcgacgtg gcggccggtg acctcggtag ctcggcggcg
      841 cgcaagttcg actgcgaggc ctggctgccg tctcagcggg cctaccgcga gctgacctca
      901 acctcgaact gcaccacttt ccaggcccgc cggctgggta tccgctaccg cgaccccagt
      961 gacccagccg ggcgccccca agtcgccgcg accctcaacg gcacgctcgc caccactcgg
     1021 tggatcgtcg ccatcctgga gaaccaccag cagcccgacg gctcggtgcg cgttccccag
     1081 gcactgcggc ccgccctggg cggcatcgag ctgatcaccg ccggctgagc tgacaagcgg
     1141 atcaccgaca gctggacatg caccagcggt ggacacaccg tgcagggcgg cacgacacac
     1201 cgggcttgag agcacgacac accgggcgtg aggggcggac acgccgggaa cgtagggtcg
     1261 agttatgcgg aggccccggt tggttgcgtc ggacgtggac ggtactttgc tcgatccgac
     1321 cgaccggatc tctgaacgta cccgggcggc ggtgcatcgg gtgcttgctg ccggtgttcc
     1381 ctttgtcctg gtcactggcc ggcccccgcg atggatacca ccgatcgtcg agcaactcgg
     1441 acatgccggg tacgcggtat gtgccaacgg cgcggtgctc tacgacgcca ctaccgaccg
     1501 cgtcagctac tcaacgacgc tggacccact gcagcttcgg gacgcggcga acgtggtggc
     1561 gaccgcgcta ccgggcgcga agctcgccgt tgagcgaccc accggcggtg ccacgctcaa
     1621 cgggttcgac gacttccttg ctgaacccga ctacacccac ccctggcccg gcgcggactc
     1681 cgcgcctgcg ccgcgcgacg tgctacttgg ccagcccgcg atcaaactgc tggttcgcca
     1741 gccggatacg agcagtgacg tgatggccgc cgcggtccgc gaaatcctcg gggccgacct
     1801 ccgggcatcg ttgaacgtca cattctccac cggccacggc ctcatcgaga tctccgcacc
     1861 cggtgtcacc aaggccaccg gcctggctcg gctggccggc gagctgggcg ttgtgccagc
     1921 tgacgtgctg gccttgggtg acatgcccaa cgacctggcg atgctgcaat gggccggctg
     1981 cggggtggcg atggccaacg cccatcccgc tgtgctcgac tgcgccgacg agatcacagc
     2041 ccgcaactcc gaggatggcc tcgcgctcgt tctttaaagg tggttttgtc cttccagccg
     2101 ccttcgtgac cgcggtggcc tccaccccac tggcagggtg gaggcc
//
LOCUS       DATADN010000741         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1004776, whole genome shotgun sequence.
ACCESSION   DATADN010000741 DATADN010000000
VERSION     DATADN010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004776"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..>2014
                     /locus_tag="VHH53_09620"
     CDS             151..>2014
                     /locus_tag="VHH53_09620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX2290431.1"
                     /translation="MITLAPPRLAPVNRAKPASPSAKASKGARATPAYRHDLDGLRGV
                     AIALVAVFHVWFGRVSGGVDVFLALSGFFFGGRLLRSALMPGASLSPTPEVMRLVRRL
                     LPALVVVLAASAVLTILIQPETRWETFADQSLASLGYFQNWELANTASDYLRAGEAVS
                     PLQHIWSMSVQGQFYIAFLALIFGFAYLLRRVLGRYLRVSFVVLLSALTIASFVYAIF
                     AHNADQATAYYNSFARAWELLLGALVGALVPHLKWPMWLRTTVAVVSLAAILACGALI
                     DGVAEFPGPWALVPVGATMLFILSGANRMADPHTNGRLPAPNRLLATAPFVSLGAMAY
                     SLYLWHWPLLIFWLAYSGKARVDFWQGAAVLAVSAVLAWLTMRYIEEPLRYRGPAPAV
                     ATTPLRSRLRRPTIALGSVVALLGVALTATSFTWREHVTVQRANGEELAGLSLRDYPG
                     AMALMDNVRVPKLPMRPSVLEAKKDLPASTIDGCISDFDNVGVINCTYGDKTAPRTIA
                     LAGGSHAEHWITALDLLGRTHNFKVVTYLKMGCPLTTEEMPLVMGDNRPYPKCHEWNS
                     RVMPKLLADKPDYVFTTSTRPWNIKDGDVMPSTYLGIWETLSRNKIPILAMRDTP"
BASE COUNT          297 a          721 c          642 g          354 t
ORIGIN      
        1 cccgcgcacc gaccgcgaca acgggcggcg gtgtcgttgc atcagccgac gcaccgatcg
       61 tgctgtatgg atgccagacg tgggcggcat cgatcgcacg gatctcggcg ggcgtcatcg
      121 gtggcacctt cggtagatta tccgtcgacc atgatcaccc tcgcccctcc ccgcctggca
      181 cccgtaaacc gggccaaacc cgcttcaccc agcgcaaagg cgtcgaaggg tgcgcgcgca
      241 accccggctt accgccacga cctcgacggg ctgcgcggtg tcgcgatcgc gctcgtcgcg
      301 gtatttcacg tgtggttcgg ccgggtgtcc ggtggcgtcg acgtcttcct ggccctgtct
      361 ggattcttct tcggcgggcg actgctgcgc agcgcgctga tgcccggcgc ttcgctatcg
      421 cccaccccgg aggtgatgcg cctggtccgc cgactgctgc ccgccctcgt ggtggtgctc
      481 gccgcctcgg cggtcctgac catcctgatt cagcccgaaa cccgttggga gacattcgca
      541 gaccagagcc tggcgagcct cggttacttc cagaactggg agctggccaa caccgcgtcg
      601 gactaccttc gcgcgggcga ggcggtcagc ccgctgcaac acatctggtc gatgtcggtg
      661 cagggccagt tctacatcgc cttcctcgcg ttgatcttcg gcttcgcata cctgctccgc
      721 cgggtcctcg gccgttacct gcgtgtctca ttcgtcgtcc tgctcagcgc gctgacgatc
      781 gcgtcgttcg tgtacgcgat cttcgcccac aacgccgacc aggcgacggc gtactacaac
      841 agcttcgcca gggcgtggga actgttgctc ggcgcgctgg tcggcgccct ggtgccgcac
      901 ctgaagtggc cgatgtggct gcgcacgacc gtcgccgtgg tgtcactggc cgccatcctc
      961 gcgtgcggcg cgctgatcga cggcgttgca gaatttccgg gtccgtgggc gctggtgccg
     1021 gtcggcgcga cgatgctgtt catcctgtcg ggggccaacc ggatggccga cccgcacacc
     1081 aacggccggc tgccggcccc caaccgactc ctggccactg ccccgttcgt gtcgttgggc
     1141 gcgatggcgt actcgctgta cctgtggcac tggccgctgc tgatcttctg gctggcctac
     1201 agcggaaagg cccgagtcga tttctggcag ggcgccgcgg tgctcgccgt gtcggccgtg
     1261 ttggcgtggc tgaccatgcg ctacatcgag gaaccgctgc gctaccgggg cccggcgccc
     1321 gccgtcgcca ccacaccgct gcggtcccgc ctgcgcaggc ccacgatcgc gctgggctcg
     1381 gtggtggcgc ttctgggcgt ggcgctcacc gccacgtcct tcacctggcg cgagcacgtc
     1441 accgtccagc gcgccaacgg tgaggaactg gccggcctgt ccctgcgcga ctacccgggc
     1501 gcgatggcgc tgatggacaa cgtgcgggtg cccaagctgc cgatgcgccc gtcggtgctc
     1561 gaggccaaga aggatctgcc cgccagcacc atcgacggct gcatcagcga cttcgacaac
     1621 gtcggggtca tcaactgcac ctacggcgac aagacggcgc cgcgcaccat cgcactggcc
     1681 ggtggctcgc acgccgagca ctggatcacc gcgctcgacc tgctcggcag gacgcacaac
     1741 ttcaaggtcg tcacctacct caagatgggc tgcccgctca ccaccgagga gatgccgctg
     1801 gtgatgggcg acaaccggcc gtatcccaag tgccacgaat ggaactcccg ggtcatgccc
     1861 aagctgctcg ccgacaaacc ggactacgtg ttcacgacct cgacgcggcc gtggaacatc
     1921 aaggacggcg acgtcatgcc gtcgacctac ctgggtatct gggaaacctt gtcgcgcaac
     1981 aaaatcccga tcctcgcgat gcgagacacc ccgt
//
LOCUS       DATADN010000742         4219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_401995, whole genome shotgun sequence.
ACCESSION   DATADN010000742 DATADN010000000
VERSION     DATADN010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401995"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VHH53_09625"
     CDS             <1..463
                     /locus_tag="VHH53_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX2290432.1"
                     /translation="AYRTRLGLERNSRMAVVVQDLVDADVAGVLFTRNPVTGAAERVI
                     EASWGLGEAVLSGLVTPDQYRLAPTGRLLERSLGEKDLQIRRSACGGMEQAPVAPELI
                     CAPCLSDGELAALHALAAACDRVFDSPDHDIEFAFRDGAVFLLQRRPITSG"
     gene            456..1889
                     /locus_tag="VHH53_09630"
     CDS             456..1889
                     /locus_tag="VHH53_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2290433.1"
                     /translation="MANGTMRGLGAALLAASLMPLNSTMIAVALPDIARDYTQSPGTV
                     TQALVSSYLMAAIVLQSPGGKLGDRLGHWRVFALGQVLIATGAVLGFVAPTLGVLALS
                     RVLMAAGGAVIVPATVALMRIELPPQRRGQAFGTFGAVMSLAAAIGPVVGGELVRAFG
                     WPSIFLANLPVLALSAVLAATARHAPVVMGRVTSPRFDWIGTVLLAAMLTSLVLGLQA
                     TGGGSIALIGTCLVLAVPFAWWQRRAADPVVAFSLFRSLPFAAGSLLVALQNLVMYTL
                     LFELPLVLEALLSLDAATTGRLLIFMMLAMVVTSLVAGRLTDWFGSRPIAVAGSLVCL
                     AGLGVLAAGSLSTLGPVRWALVILGIGIGLVTPAAQNATLSAVSQERSGMAAGVSSTM
                     RYLGGITGVAVLGRVLDLSSDRDAIISEHHTMLAVFAATLLVGLACAALLPGRRPAPA
                     SPSSAGARSNRSASRYSRIAGRRAGDQ"
     gene            2674..2958
                     /locus_tag="VHH53_09635"
     CDS             2674..2958
                     /locus_tag="VHH53_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290434.1"
                     /translation="MRRKKIDKAAVLRNLQTIENDDYDGFVLLVQAARNRSAAFDPDL
                     PVWDMAQHMGIIVDCNRSGVPEFDEQMRDFVRDRNHVDGSDKRPFVKGRH"
     gene            3116..4111
                     /locus_tag="VHH53_09640"
     CDS             3116..4111
                     /locus_tag="VHH53_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2290435.1"
                     /translation="MTAPLEGSGSPAHAQPEAVATGMGTAIQGRSLGQIAWLRLKRDK
                     IALAGGGVIVTLILVALFAPAIVAVLGHPPLEFHYEKVDADLQVAVGNFGGISPDFLF
                     GVEPVNGRDVFSRIVYGSRISLLIAFLATLLSVVLGTVMGVVAGYFGGWLDTLISRTM
                     DVFLAFPLLLFAIALAGVVPDQAFGLEGDTLRIALMVFIIGFFNWPYIGRIVRGQALS
                     LREREFIDAARSLGARSPRILFTEMLPNLAAPILVYSTLLIPTNILFEAALSFLGVGV
                     RPPTPTWGGMLAEATHWFQIAPNFMLFPGLAIFITVLAFNLFGDGLRDALDPRSR"
BASE COUNT          630 a         1331 c         1481 g          777 t
ORIGIN      
        1 ggcctaccgg acgcggctcg ggctcgagcg gaactcccgg atggcggtgg tggttcagga
       61 tctggtggac gccgacgtgg ccggcgtcct gttcaccagg aacccagtca cgggagcggc
      121 ggaacgggtg atcgaagcaa gctgggggct gggcgaggcc gtcctctcag ggctggtaac
      181 gccagaccag taccggctgg ctcccaccgg gcggctacta gagcgcagtc tcggtgagaa
      241 ggatctgcag atccggcgca gcgcctgcgg cggtatggag caggcgccgg tagcaccgga
      301 gttgatctgt gcaccgtgcc tgagcgacgg ggaactcgcc gcgttgcatg cgctcgcggc
      361 ggcgtgcgac cgcgtcttcg attcgcctga ccatgacatc gagttcgcct tccgggacgg
      421 tgcagtgttt ctcctgcagc gacgaccgat caccagtggc taacgggacc atgcggggcc
      481 ttggagcggc gttgttggcc gcgtcattga tgcccctcaa ttcgaccatg atcgcggtgg
      541 cgttgccgga catcgcacgc gattacacac agtctccagg caccgtgacg caggcgctgg
      601 tgtcgagcta cctcatggcg gcgatcgtcc tgcaaagtcc gggaggcaag ctcggtgacc
      661 ggctcgggca ctggcgggtg ttcgcgctgg gccaggtgct gatcgcgacg ggcgccgtgc
      721 tcggcttcgt cgcgccgacg ctgggcgtgc tggcgctgtc ccgggtgctg atggccgccg
      781 gcggtgcggt gatcgtaccg gcgacggtgg ccctgatgcg catcgagctg ccgccgcaac
      841 gcaggggcca ggcgttcggc acgtttggag cggtcatgtc gctcgcggcg gcgatcggac
      901 cggtggtcgg cggtgagctg gtccgagcgt tcgggtggcc gagcattttc ctggccaacc
      961 tgccggtgct cgccttgtca gcggtactgg cggcgacggc cagacatgca cccgtggtaa
     1021 tggggcgcgt gacgtccccg cggttcgact ggattggcac agtactgctc gccgcgatgc
     1081 tgacgtcgct ggtgctgggt ttgcaggcca cggggggcgg ctcgatagcc ctcatcggta
     1141 cctgcctcgt cctggccgtg ccgttcgcgt ggtggcaacg gcgcgcagcg gatccggtcg
     1201 tcgcgttctc gttgttcagg tccctgccgt tcgcggcggg cagcctgctg gtggcactgc
     1261 agaacctggt gatgtacacg ctgctgttcg agcttccgct ggtccttgag gcgctgctgt
     1321 ccttggacgc cgcgaccact ggccggctgc tgatcttcat gatgttggcg atggtggtca
     1381 cctcgttggt ggccggccgg ttgacggact ggttcgggtc gcggcctatc gcggtcgccg
     1441 gttcactggt ctgccttgcg ggcctgggcg tgctcgctgc cggcagcctc tcgacgctcg
     1501 gtccggtgcg gtgggcactg gtgatcctcg gaatcggcat cggccttgtc accccggcgg
     1561 ctcagaacgc cacactcagc gcggtttccc aggagcgcag cggcatggcc gccggggtca
     1621 gctcgacgat gcgctatctc ggcggcatca caggtgttgc cgtcctcggc cgcgtactcg
     1681 acctcagcag cgaccgtgac gcgatcatca gcgagcacca caccatgctt gccgtgttcg
     1741 ccgcgaccct gctggtgggg ctggcatgcg cagcgctgct tccgggccgg cgcccggctc
     1801 cggcgtcgcc gagcagtgct ggggccaggt ccaaccgctc ggctagccgg tactcacgga
     1861 tcgcagggcg ccgagctggc gaccagtgag ccgatcgagc ccaccgacgt cgtcgcgtcg
     1921 gcgggcaggt agacaccagc cgctggtagt cggggtcggg aactcgaggg tctcgaacgc
     1981 cagcggcagg aggcccacct ccgggggtga catcccaatc aggaccagga ccgcccttcc
     2041 ttccatctga ccgaaatcgc cggctatccc gaccccctcg ggtcgaggtc gaacgcggac
     2101 atacatggtt gtgacgttga tattaagact gcggaccggc agagcgtctc gatcacctac
     2161 gattcgaaag agttcaacag caacccgcag cagtcgggtg aggcggccaa gcagatcgct
     2221 gccgcggtgg tgattaatgt accgctgaag aattgatacg gggcggagca cgccgagccc
     2281 cgcctccggc accatcgtgc gaacggcaaa gcctcgcggt gacacactgg cccccttacc
     2341 gctgacgcgt tggtcacagt tcgctggcac gttagccgtt gggcgcagat gttccggtgg
     2401 cgggacgttc ggcttcggcc gctgcgcagg gaccagcttg ccctcggggc ttcgccaccg
     2461 agcaagcaag gcgtggatct cccgctccct gtccgagtgg tcgacgggga gcgcgccgga
     2521 gcgtcccgca acgaaccgcg gcgtgccgtg ggcacgcgct cggtggtcga ccgccaggga
     2581 agcctcgcgc aacgtcagcc tagggtatga tcgatacgat cattccataa cattaattgt
     2641 tctaccgcgc atccagattg gggagggtgg ccggtgaggc gcaagaagat tgataaggcc
     2701 gccgttctgc gaaacctgca gacgattgag aatgacgatt atgatggctt cgtgctctta
     2761 gtacaagccg cgcgtaacag aagtgccgca tttgatccgg acttgccggt gtgggacatg
     2821 gctcaacata tgggcatcat cgtggactgt aaccgtagcg gtgttccgga attcgatgaa
     2881 caaatgcgag atttcgtgcg ggacaggaat cacgtggacg gctcggacaa gcgtccattc
     2941 gtcaagggac ggcactaagg agtgcaagag cctggcatcg cagccagccg acggctggta
     3001 ctggtgaggc tctcgagcgc cgtgcttcta cgatgtccgc gacgtccgcg cgcacacgaa
     3061 acaggggtac gagacggatg aactggtcgg gggcggcccg cgcgacggtt cggccatgac
     3121 cgctccgctg gagggctccg gctcgccagc ccacgcccaa cccgaggctg ttgcgacggg
     3181 aatgggcacc gcgatccagg gccgttcact gggtcagatc gcctggctgc gcctcaagcg
     3241 ggacaagatc gccctggccg gtggcggcgt catcgtgacc ttgatcctgg tggcgctctt
     3301 cgcgccggcg atcgtcgcgg tcctcgggca tccaccgctg gagttccact acgaaaaggt
     3361 cgacgccgac ctgcaggtgg ccgtcggcaa cttcggcgga atcagcccgg acttcctgtt
     3421 cggagtcgag ccggtcaacg gccgcgacgt gtttagccgc atcgtctatg ggtcacggat
     3481 ctcactgctc atcgccttcc tcgcgaccct gctgtcggtg gtgctgggca ccgtgatggg
     3541 cgttgtcgcg gggtacttcg ggggttggct cgacacgctg atcagccgga cgatggacgt
     3601 cttcctggcg ttcccgctgc tgctgttcgc gatcgcgctg gccggggtgg tgccggacca
     3661 ggcattcggc ctggagggtg acaccctgcg gatcgcgctg atggtgttca tcatcgggtt
     3721 cttcaactgg ccctacatcg gccggatcgt ccgcgggcag gcgctgtcgc tgcgcgagcg
     3781 cgagttcatc gacgccgcgc gcagtctcgg cgcgcgcagc ccgcggatcc tgttcaccga
     3841 gatgctgccc aacctggccg cgccgatcct ggtctactcg acgctgctga tcccgaccaa
     3901 catcctgttc gaggcggcgc tgtcgttcct cggcgtcggg gtgcggccgc cgacccccac
     3961 ctggggcggg atgctggccg aggccacgca ctggttccag atcgcgccga acttcatgct
     4021 cttccccggg ttggcgatct tcatcacggt gctggcgttc aacctgttcg gcgatggtct
     4081 gcgcgatgcg ctcgatccgc ggtcgcgatg atgacgaagg ggtaccagat gagaacgagt
     4141 cgccgagtcg tggttggcgc ggcagcgacg atggtggtgg cagcgctggt gctggctggg
     4201 cccgccgcac ccagccagc
//
LOCUS       DATADN010000743         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1454854, whole genome shotgun sequence.
ACCESSION   DATADN010000743 DATADN010000000
VERSION     DATADN010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454854"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..634
                     /locus_tag="VHH53_09645"
                     /pseudo
     CDS             202..634
                     /locus_tag="VHH53_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336223.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
     gene            complement(645..1529)
                     /locus_tag="VHH53_09650"
     CDS             complement(645..1529)
                     /locus_tag="VHH53_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEX2290436.1"
                     /translation="MTGATPVPEHSGGRDRILTAIPPAGSVPASRDAWEATPRAEEVQ
                     AGIVALWRAYGKDRNATLRDRLLLHYAPLVKYVAGRVGTGLPAHVDIADLVQSGVFGL
                     WEAIDRYEPERGLKFETYAMPRIRGAILDELRAQDWVPRSVRSRARDVERALERLECR
                     LQRSASDTEVAAELGITVTDLRELYAQLQLTSVVALDELIAVGRGGTSIAETLPDDQM
                     DEPGAILDWVEGRRLLAEAISQLTDRDRVVVSLYYFENLTLAEIGRVLGVTESRACQL
                     HTRAVLRLRSKMVDASAG"
     gene            complement(1504..2403)
                     /locus_tag="VHH53_09655"
     CDS             complement(1504..2403)
                     /locus_tag="VHH53_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX2290437.1"
                     /translation="MAAYRDRFTAHLRSVRGLSPHTVRAYCGDVTSLLHHLVSSGRDT
                     LAALDLTLLRGWMGTQYRSGHRRSTLARRTAAARAFTSWATRAGLLASDPGLRLTAPR
                     SHRVLPPVLHPEQAAAALHAAQRGTAEADPVAVRDHAVVELLYATGIRVAELCGLDLG
                     DLDEQRRLVRVFGKGAKERAVPYGLPAQRALRRWIEQGRPALVQSHSAAALFLGARGG
                     RVDQRIVRAVVHEVIGAVAGVPDMGPHGLRHSAATHLLEGGADLRTVQELLGHATLAT
                     TQLYTHVTVERLKAIHDRSHPRS"
     gene            complement(2478..>2706)
                     /locus_tag="VHH53_09660"
     CDS             complement(2478..>2706)
                     /locus_tag="VHH53_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA processing protein DprA"
                     /protein_id="HEX2290438.1"
                     /translation="KRAPLPYDGLSPELLAVYEALPVRACRYPEQLVRECGVPLHRVR
                     SALPLLELQGLVACGVTGWYRISQDPTRGGA"
BASE COUNT          413 a          942 c          925 g          426 t
ORIGIN      
        1 ctctgtgtcc acatcggcgg gcttcgccgc tgggcggtcg cgaccggcgc cgggacgctg
       61 tcgggcatgt ctcgggttct gatatgcgcg ctgctcgtca ccgtgctgtc cggtgcgacg
      121 gggactggga tggccgcttc ggcaggcttt gcggcgcagc ctgggacggc tgatgccggt
      181 gaccagtccc ggccgcacac ccggatgttc ggttggccgc tggcgggacg tccggccgtg
      241 gtccgggctt tcgaggcacc ggcgggtgcc gtacgggccg gggcatcgcg gtgtcgacct
      301 ggcgtccgtc cccggggctg cggtgctcgc cgccggcgcc ggcatggtgg ttttcgccgc
      361 ggttgtggcc ggccgcggtg tcgtgtcggt ggaccacccc ggcgggttac gcacgaccta
      421 tgagccagtg tcaacgatcg tcgctgccgg tgatcaggtg gccagaggtg agcagctggg
      481 tgccgtgcag ctcggccacc ctggctgcac cgctactgcc tgcctgcact ggggtgtacg
      541 gcgcggtccc agcgattacc tggatccgct gcgcctggtt gtgatgccac gagtgcggct
      601 gctgcccatt gacggcctgc ccaccgcccg gtgatgccca ggattcagcc ggcggaggcg
      661 tcgaccattt tgctccgtag ccgcagcacc gctctggtgt gcagttgaca agccctcgac
      721 tcggtaaccc cgagcacccg gccgatttcg gcaagggtga ggttctcgaa gtagtacagg
      781 gacaccacca cccggtcccg gtcggtgagt tgcgagatcg cctcggccag caaccgccgg
      841 ccctcgaccc aatccaggat tgcgcccggt tcgtccattt ggtcgtcggg caacgtctcc
      901 gcaatcgagg tgccaccgcg gccaacggcg atgagctcat ccaacgccac cacgctggtc
      961 aactgcagct gtgcgtagag ctcccgcagg tcggtaacgg tgatgccgag ctcggcagcc
     1021 acctcggtgt cgctggccga acgctgcaac cggcattcca gacgttccag tgcgcgctcg
     1081 acatcccgag cccggctgcg caccgaacgg ggcacccagt cctgggcccg cagctcgtcg
     1141 aggatcgcgc cgcggatccg cggcatcgcg taggtctcga acttcaaacc acgctcgggc
     1201 tcgtaccgat cgatggcctc ccacaggccg aacactccag attgcacgag gtcggcgatg
     1261 tcgacgtggg caggcaagcc ggtacccacg cggccggcca cgtacttcac cagcggcgcg
     1321 tagtgcagga gcagcctgtc acgtaatgtc gcattgcggt ccttgccgta ggcgcgccac
     1381 aacgcgacga tgccggcttg cacctcctcg gccctgggcg tggcctccca ggcatcccgg
     1441 ctagcgggca cagaaccggc tggggggatg gcggtcagga tgcggtcgcg acccccggaa
     1501 tgctcaggaa cgggggtggc tccggtcatg gatcgccttc agcctctcga cagtgacgtg
     1561 cgtgtagagc tgcgtggtgg cgagcgtagc gtgaccgagt aactcctgaa cggtgcgaag
     1621 gtctgcgccg ccctccagca gatgagtcgc ggcgctgtgc cgcagcccgt gcgggcccat
     1681 atccgggacg cccgccaccg cgccgatcac ctcgtgcacg accgctcgaa cgattcgttg
     1741 atcgactcgc ccccctcgtg ctccgaggaa cagcgccgcg gctgaatggg attggacaag
     1801 ggcggggcgt ccctgttcga tccagcgacg cagagcgcgc tgcgcgggta acccatacgg
     1861 cactgcgcgt tccttcgcac ctttgccaaa cacccggacc aggcgacgtt gctcatcgag
     1921 gtcaccgaga tcaagcccgc agagctcggc gacacgtatc cccgtcgcat acaacaactc
     1981 cacaacggcg tgatcacgta cggcaacagg atccgcctcg gcggtgcccc gttgcgccgc
     2041 atggagtgcc gccgccgcct gttccgggtg cagcaccgga ggcaggaccc ggtgtgaccg
     2101 cggcgcggtc agccgcagcc ccgggtcgct ggccagcaga ccggctcggg tggcccacga
     2161 agtgaaggcg cgtgccgctg cggtccgccg tgccagcgtg ctgcgccggt gaccgctgcg
     2221 gtactgcgtg cccatccagc cgcgcagaag cgtgagatcc agcgcagcca gcgtgtcgcg
     2281 acctgaactc accaggtggt gcagcaggga ggtgacgtcg ccgcagtagg cccgcacggt
     2341 gtgcggtgac aagccgcgca ccgaacgcag atgggccgtg aaacggtctc ggtacgcggc
     2401 caggtcgtcc ggtagtgagt cgggtgccgg cggttcctcg ctcaccggca ggacggtgcg
     2461 ctcccggccg ccggaggtca agcaccgcca cgcgtgggat cctgcgatat tcggtaccag
     2521 cccgtcactc cacaggcgac cagcccctgc aactccagca acggcagcgc ggaccgcacc
     2581 cgatgcagcg gtaccccgca ctcacgcacc agttgctcgg gataccggca agcccggacc
     2641 ggcagcgcct cgtacaccgc gagcaactcc gggctcagcc catcgtaggg caacggggcg
     2701 cgctta
//
LOCUS       DATADN010000744         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1004866, whole genome shotgun sequence.
ACCESSION   DATADN010000744 DATADN010000000
VERSION     DATADN010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004866"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="VHH53_09665"
     CDS             complement(<1..1426)
                     /locus_tag="VHH53_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2290439.1"
                     /translation="MTNHWRDIRHADLILINGANPAEAHPVGFRWMMRAKLDRGAKMI
                     HADPRFTRTSAVADIHLRIRTGSDVAYFGGLINYVLQNNLYHKEYVEFATNAGFVVSP
                     NYSFNDGMFNGFDAQTGSYDPRAWSYELDGEFAKVDIAHPRSVLNLMREHYSRYTPEM
                     VERVTGIPRDQFLEVARQVGEMGRPDKVMTIVYAVGLTHHTTGVQLIRSAALLQLLLG
                     NMGRPGGGMNAERGHANIQGNTDHAISWEILPGYLRIPAPGQKNLADYLAASATKKLR
                     PNSVNFFGENYRKFMVSLLKAWYGDAATKENEFAFHHIPKPAANASWISIFDQALRGR
                     MEGLVLSGMTATSIGPDSNQVLTALSKLKWLCVLDPFATTSSEFWQAPGVDPAGVRTE
                     VFMLPATHWIEKDGSFTNSGRWAQWKEQILPPDGEARHDHWIMAELFQRVRRLYQQQG
                     GKYPAPVLQLTMPYRDPIRPELDEI"
     gene            complement(1475..>1725)
                     /locus_tag="VHH53_09670"
     CDS             complement(1475..>1725)
                     /locus_tag="VHH53_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290440.1"
                     /translation="RAPGATEWRTVSWDFTLDKLARNIKASRDRTFVTQDANGNVVNR
                     CEGIAFAGGAAFSSEEGYFAAKLMRGLGVVHIEQQARV"
BASE COUNT          263 a          559 c          583 g          320 t
ORIGIN      
        1 ctatctcgtc cagctcgggt ctgattggat cccggtaggg catggtcagt tggaggaccg
       61 gtgccgggta cttcccgccc tgttgctgat agagacggcg tacccgctgg aagagctcgg
      121 ccatgatcca gtggtcgtgg cgggcctctc cgtccggcgg caggatctgc tccttccact
      181 gggcccaccg gccgctgttg gtgaaggacc cgtccttctc gatccagtgc gtggccggga
      241 gcatgaacac ctcggtccgc acgccggccg ggtcgacgcc cggcgcctgc cagaactcgg
      301 agctggtggt ggcgaaggga tccagcacgc acagccactt cagcttcgac agcgccgtga
      361 gcacctggtt ggagtcgggg ccgatgctcg tggccgtcat cccggagagg accagcccct
      421 ccatccgccc gcgcagcgcc tggtcgaaga tcgagatcca ggacgcgttg gcggccggct
      481 tggggatgtg gtggaaggcg aactcgttct ccttcgtcgc cgcgtcgccg taccaggcct
      541 tgagcaggct caccatgaac ttgcggtagt tctcgccgaa gaagttcacc gagttcggcc
      601 gcagcttctt cgtcgcgctc gccgcgaggt agtcggccag gttcttctgc cccggcgcgg
      661 gaatcctcag gtatccgggc aggatctccc aggagatcgc gtggtcggtg ttgccctgga
      721 tgttggcgtg gccgcgctcg gcgttcatgc cgccaccggg acgccccatg ttgcccagca
      781 acaactgcag cagcgccgcc gagcggatga gttggacgcc ggtggtgtgg tgggtcaggc
      841 cgaccgcgta gacgatcgtc atcaccttgt ccggccggcc catctcgccg acctgcctgg
      901 ccacttccag gaactggtcg cgcgggatgc cggtaacgcg ctcgaccatc tccggggtgt
      961 accggctgta gtgctcgcgc atcaggttga gcaccgagcg cgggtgcgcg atgtccacct
     1021 tggcgaactc gccgtccagc tcgtagctcc acgcccgggg gtcatagcta ccggtctgcg
     1081 cgtcgaaccc gttgaacatc ccgtcgttga acgagtagtt cgggctcacc acgaagccgg
     1141 cgttggtggc gaactcgaca tattccttgt ggtacaggtt gttctgcaac acgtagttga
     1201 tcaagccgcc gaagtaggcg acgtcggatc cggtgcggat ccgcaggtgt atgtccgcga
     1261 ccgcggacgt gcgcgtgaag cgcgggtcgg cgtggatcat cttcgcgccg cggtccagct
     1321 tggcgcgcat catccagcgg aaaccgaccg ggtgggcctc ggcggggttg gccccattga
     1381 tcagaatcag gtcagcgtgc ctgatgtcgc gccaatggtt ggtcatggcc cctcggccga
     1441 acgtggcggc caaactgacc accgtaggac cgtgtcaaac acgggcctgt tgctcgatat
     1501 ggaccacccc caagccccgc atcagcttgg cggcgaagta gccctcctcc gagctgaacg
     1561 cggcaccgcc ggcgaaggcg atgccctcgc agcggttgac gacgtttccg ttggcgtcct
     1621 gggtgacgaa cgtccggtcc cgcgacgcct tgatgttccg cgcgagcttg tccagggtga
     1681 agtcccagga gaccgtgcgc cactcggtgg caccgggtgc gcgga
//
LOCUS       DATADN010000745         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1004995, whole genome shotgun sequence.
ACCESSION   DATADN010000745 DATADN010000000
VERSION     DATADN010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004995"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VHH53_09675"
     CDS             complement(<1..861)
                     /locus_tag="VHH53_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2290441.1"
                     /translation="MLGEASGEPGTVRAVRLAQQLPPGLLDRFTLIGLARRGTGPSEP
                     LDCIPKTVRSQIVGIDPDVSEPGQLDSLLDTTRAAIQTCVQDLGEVLTAINSTSTADD
                     LEQLRVTLNAPVLNVVSHGEASRAVTDFIRRYPNSVGRVVLDGAVDPTLDGIAAAEAT
                     LAAAEAGYTAFAADCVARDCPLGPDPSTALPTLAQALRQAPLQVDWQQITAGTAYYAV
                     LETVGDPQRWAQLSSALATARDGNGVQLATLVEPMLAKTQGLPARFDPALATHCNDTS
                     TRVPPERAQQL"
     gene            903..1043
                     /locus_tag="VHH53_09680"
     CDS             903..1043
                     /locus_tag="VHH53_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290442.1"
                     /translation="MSEFPNAGRDSVASTRSSLHSTRRGLGVPSAAEIPLVHSCHGSE
                     LR"
     gene            complement(1318..1707)
                     /locus_tag="VHH53_09685"
     CDS             complement(1318..1707)
                     /locus_tag="VHH53_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2290443.1"
                     /translation="MRITASAVALNVEDVAGSSAFLVDHFGFHEEMAADGFASLTRAD
                     AGMNVAFLRRGLASLPKDQRDDHAGGLILAFVVEDLEGELARLRAEGVAITMPLTSEE
                     WGEQAFQVRDPNGVIVQLVDWNAHTGD"
     gene            1804..2646
                     /locus_tag="VHH53_09690"
     CDS             1804..2646
                     /locus_tag="VHH53_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2290444.1"
                     /translation="MSVERSSAGDPARTLELLWRVPPRARRGPRQGLSIDAVVRAATT
                     LADSEGLAAVTMRRVAHALGVAPMTLYTYVPSKAELVDLMLDAAYAQMPRTGTTGQPW
                     RQRVTAIAEENRALFEAHPWTATVSTTRPPLGPGLMAKYEHELSALDGLGLDDVEMDA
                     ALTYLLSFVQSCARDATAVRISQQDSAMDDQQWWAANAPLLVRVFDAEAYPIAARVGA
                     AAGAAYGSAYSPEHAYDFGLRRVLDGLAALIESPTRNSAPNGDCAGTAVRDSAPNCAQ
                     DGSV"
BASE COUNT          423 a          887 c          925 g          437 t
ORIGIN      
        1 cagctgctgg gcccgctcgg gcggtacccg ggtcgaggtg tcgttgcagt gggttgccag
       61 ggctgggtca aagcgcgccg gcaacccttg agtcttcgcc aacatcggct ccacgagcgt
      121 ggctagctgt accccgttgc cgtcacgggc cgtcgccagt gcgctggaca actgcgccca
      181 gcgttgcgga tcgccgaccg tttcgagcac cgcatagtag gccgtgcccg cggtgatctg
      241 ctgccagtcg acctgcagcg gagcctggcg cagcgcctga gccagggtcg gcaaggcggt
      301 gctggggtcg ggtcccagcg ggcaatcccg ggccacgcag tcagcggcga acgccgtgta
      361 gcccgcctcc gctgctgcca gcgtggcctc cgctgcagca atgccatcga gcgtgggatc
      421 gacggcaccg tcgagcacca cccgcccgac cgagttggga taacggcgta tgaagtcggt
      481 gaccgcccgg gacgcctcgc cgtgcgagac cacgttgagc accggcgcgt tcagagtaac
      541 ccgcagctgc tcgaggtcgt cggcggtgct cgtcgaattg atcgccgtca gcacctcgcc
      601 gaggtcctgc acgcaggtct ggattgcggc gcgggtcgtg tcgagtagcg aatccagctg
      661 gccgggctcg ctgacgtcgg gatcgatacc gacgatttgg ctccggaccg tcttcggtat
      721 gcaatccaac ggttcgctcg gcccggtgcc gcggcgagcc agcccgatca aggtgaaacg
      781 atcgaggaga ccgggtggca gctgctgagc cagccggacc gcccgaaccg tacccggctc
      841 cccggacgcc tcgccgagca ccgagggggg caggaccctc gccgacccgg gtcaggtcca
      901 gcttgagcga attcccgaac gctggccggg acagcgtggc atcgacccgc agttcgctgc
      961 actccacccg tcgtgggctc ggtgtgccca gcgcagcgga gatcccgctg gtgcactcct
     1021 gccacggcag cgaattgcgg tagtactcgc cgggggccgg cagcggtgtg gcgctgggcg
     1081 caacctccac ggggcgttgc ggttgcaggt tggagtcccg caccgccacc gcaggccggt
     1141 cggacgggcc cgcagtgcac cctgccagga gcactgccaa gagcagcacc gccagcactc
     1201 gcgggtaccg cggggtccgg cgcaggcgaa cgggcacgga ctctcctcgc atccagatct
     1261 ggcggtgtgg cgatgctgac agtgccgcgg tgagcctgcg gtgagcatgc gagcggttca
     1321 gtcgccggta tgggcgttcc aatcgacgag ctggacgatc acgccgttgg gatctcttac
     1381 ctgaaacgct tgctctcccc attcttcgct ggtcagcggc atggtgatgg cgacgccttc
     1441 ggcacgcagt cgggccagct cgccttcgag gtcctcgacg acgaacgcga ggatcaggcc
     1501 gcccgcgtgg tcgtcgcgtt gatctttcgg cagggacgca aggcccctgc gcaagaaggc
     1561 gacgttcatc ccggcgtccg cacgtgtcag cgatgcgaag ccgtcggccg ccatctcctc
     1621 gtggaagccg aagtgatcaa ccagaaaagc gctggacccc gcgacgtcct cgacgttcaa
     1681 agcgacggcg gaagcggtga tgcgcatggg ttctcctcaa gcggctgcgg tggcgacacg
     1741 cgggcagggt ccgagcatcg tactttgtat accgtacatc gtacgacatg gcacgatgtt
     1801 ccggtgtccg tcgaacgcag cagcgctggc gacccggctc gtactcttga gctgctctgg
     1861 cgagtgcccc ccagagcacg gcgcgggcca cggcaaggtc tgagcatcga cgctgtggtg
     1921 cgtgcggcga cgactctggc ggacagcgag ggcctggcgg ccgtgacgat gcggcgagtg
     1981 gcccacgcgc tcggtgtggc gccgatgacg ctgtacacct acgtcccgag caaggccgaa
     2041 ctagtggatc tgatgctcga cgcggcctac gcgcagatgc cccgtaccgg cacgacgggg
     2101 cagccctggc ggcagcgagt gaccgccatc gccgaggaga acagggcgtt gttcgaggcg
     2161 cacccctgga cggccaccgt ctccaccacc aggccaccac tgggtcccgg gctgatggcc
     2221 aaatacgagc acgagttgtc agcgttggat ggcctcggcc ttgacgacgt cgagatggat
     2281 gccgcgctga cctatctgct cagtttcgtc caaagctgcg ctcgcgacgc cactgccgtt
     2341 cgcatctccc agcaggacag cgcaatggac gaccaacaat ggtgggcagc caacgcgccg
     2401 ctgctcgtca gggtcttcga cgccgaggct tatccgattg cggcccgcgt gggtgccgcg
     2461 gccggtgccg catatggcag tgcgtacagc cctgagcacg cttacgactt cggtctgcgt
     2521 cgtgtcctcg acggtctcgc ggccctcatc gagtcgccca cccgcaattc ggctccgaat
     2581 ggcgattgtg cgggcaccgc tgtgcgcgat tcggctccga attgcgcaca ggatgggtcg
     2641 gtttagtggg tgagtacgtc ggtgagggcg tc
//
LOCUS       DATADN010000746         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_402278, whole genome shotgun sequence.
ACCESSION   DATADN010000746 DATADN010000000
VERSION     DATADN010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402278"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="VHH53_09695"
     CDS             complement(<1..1531)
                     /locus_tag="VHH53_09695"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX2290445.1"
                     /translation="MTLSGARSHAGDPDTSAGDLVRARAALLGANPRQRQLPAHALRA
                     ALVDLHELWLTSRCTSLGFGRGTALVAVGALGRSELVPYSDLDLLLLHDGKRDVSELA
                     DSLWYPLWDARIDLDHSVRTVGQAVQVAATDLRAALGLLDARVIVGDGALFTTMDTAV
                     RRAWRTGIRTRLEELAESAQQRWLRSGDIAHRVEPDLKNGHGGLRDVQLLDALSAGQL
                     LDRAGPEVSAARDLLLDVRTELHRLAGRSRDVLRAQDADEVATTLGLGDRFDLARSLS
                     GAARTVAYAMDVGLRAARAALPPRGRARLTGLRTLGRRPVRRPLDDGVVEHGGEVALA
                     RDTNPARDPALALRVAATAARTGMPVATGTLTTLADTAPELRRPWPRPALEGLLSLLG
                     TGQPAVEVIEAMDRTGLWGRLFPEWGAVRDLPPRDRVHVWTVDRHLVETAAQAARLTT
                     TVARPDLLLLGALLHDLGKGRGGDHSIVGAALAEQIGHRLGLPPSDVAVLCAMVRHHL
                     LL"
     gene            complement(1854..2192)
                     /locus_tag="VHH53_09700"
     CDS             complement(1854..2192)
                     /locus_tag="VHH53_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX2290446.1"
                     /translation="MKLVTAIVKPFTLEDVKGALEQIGVLGMTVSEVQGYGRQKGHTE
                     VYRGAEYSVDFVPKIRVEVVVDDMQVDKTLDVLVESARTGKIGDGKVWVTPVETVIRV
                     RTGERGVDAL"
     gene            complement(2199..>3031)
                     /locus_tag="VHH53_09705"
     CDS             complement(2199..>3031)
                     /locus_tag="VHH53_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX2290447.1"
                     /translation="DFAGGTAVHINAGAAGLALCLVLGRRRGWPREAMRPHNLPFVML
                     GAGLLWFGWFGFNAGSAVAANGVAGLAFVNTMVATAAAMLGWLLVERVRDGRPTSLGA
                     ASGVVAGLVAITPACSSVSPTGAIVVGAIAGAVCALAVSIKFRFGFDDSLDVVAVHLV
                     GGLVGTLLIGFLATSVAPAGVDGLFYGGGTDQLWRQAVAAGAVLAYSFLVTLIIALVL
                     KYTIGLRLGTDAEQAGIDETEHAEAGYELSSIFTSSGNSRVTQPAAPSKISASAGQEG
                     "
BASE COUNT          521 a         1102 c          964 g          444 t
ORIGIN      
        1 gcagcagcaa atggtgacgc accatcgcgc agagaactgc aacgtctgag ggcggtagcc
       61 cgagccggtg cccgatctgc tcggccagcg ccgcgccgac gatggagtgg tcaccgccac
      121 ggcccttgcc caggtcgtgc aacagggcac cgagtagcag cagatccggg cgagcgaccg
      181 tagtggtcag ccgcgctgcc tgtgccgcgg tctccaccag atgccggtcc accgtccaga
      241 catgcacccg atcccgcggc ggcagatcac gcaccgcacc ccactccggg aacagccgcc
      301 cccacagccc ggtgcggtcc atggcctcga tgacttctac cgccggctga ccggtgccca
      361 gcaacgacag caatccctcc aacgctgggc gcggccaggg tcgacgtagc tccggcgcag
      421 tgtcggcgag cgtcgtgagg gtgccggtag cgaccggcat accggttcgc gccgcggtcg
      481 ccgcaactcg caacgccagc gccggatccc gcgccgggtt ggtgtcgcga gccagcgcca
      541 cctcaccccc gtgctccacc accccgtcgt ccaacgggcg gcgtaccggt ctgcgcccca
      601 gggtccgcaa cccagtcagc cgggcacggc cccgcggggg cagcgcggcg cgtgcagcgc
      661 gcaacccgac gtccatcgca taggccacgg tccgagccgc tccggacaga cttctggcca
      721 agtcgaaccg gtcaccgaga cccagggtgg tagcgacctc gtcagcgtcc tgggcccgca
      781 acacatcgcg acttcggccg gcgagccggt gcagctcggt gcgcacatcc agaaggaggt
      841 cgcgcgcggc ggacacctcg ggcccggccc gatccaggag ctgacctgcg gataacgcat
      901 cgagcagctg gacatcccgc agcccgccgt ggccgttctt gaggtctggt tccacccggt
      961 gcgcgatgtc cccgctgcgc agccagcgtt gctgcgccga ctcggcaagt tcttcgagcc
     1021 tggtgcggat gccggtgcgc caggcccgcc gcacagcggt gtccatggtg gtgaacagcg
     1081 caccgtcccc gacgatcacg cgggcgtcga gcaatcccag tgcagcgcgc agatcggtgg
     1141 ccgccacctg cacggcctgc ccgacggttc gtacgctgtg atcaaggtcg atgcgagcgt
     1201 cccacagcgg ataccacagc gagtcggcga gctcgctcac gtcgcgcttg ccgtcatgca
     1261 gcagcaacag gtcaaggtcg gaatacggga ccagctcgct gcggcccagt gcaccgaccg
     1321 caaccagcgc ggtaccgcgg ccaaaaccca gcgacgtgca tcgggatgtc aaccacagtt
     1381 cgtggaggtc caccagcgcg gcccgtaatg cgtgcgctgg cagctggcgc tgccgcgggt
     1441 tagctccgag cagtgcggca cgggcccgga ccaggtcgcc tgcggaggtg tccggatcac
     1501 cggcatgaga ccgggccccc gacagcgtca tgtgcgcttc gcctttccgt attggcgtcg
     1561 tcacgccgcc cagcgacgag aattcgtacg agtctggcgt gcggtaccgg gtccctcgac
     1621 tgcaccccga cctcagctgt tgaggcttcg cgaaatgtgt ttcgtttgct gaaacgccgc
     1681 gtttcagtga ggtaaatggc tctgcttccg agcaggctgc ggcatcaaat acctcggtga
     1741 ctaggctggc ggaatctagc gggacaccgg caacgtgcac cggtgccccg ccacatgtca
     1801 ggctccgcgc tggctttgca cggcgtcagc tccaggacac ccgctcctga agatcacagc
     1861 gcgtccacac cccgttcccc ggttcgaacc cggatcacgg tctccactgg tgtgacccac
     1921 actttcccgt ccccgatctt gccggttcgg gctgattcga ccagcacgtc aagagtcttg
     1981 tccacctgca tgtcgtccac tacgacctcg acgcggatct tgggcacgaa gtccacggag
     2041 tactccgcgc cccggtagac ctcggtgtgc cctttctgcc ggccgtagcc ctgcacctcg
     2101 ctgaccgtca tgccgagtac cccgatctgt tccagagcac ccttgacgtc ctccagcgtg
     2161 aacggcttga caatcgcggt caccagcttc atcgccactc acccttcctg accggccgag
     2221 gccgagatct tactgggggc ggctggctgc gtgacccggc tgtttccgct actggtgaag
     2281 attgagctga gctcgtaacc ggcctcggcg tgctcggttt cgtcgatacc ggcctgctca
     2341 gcatcggtgc ccagccgcag gccgatggtg tacttcagca ccagtgcgat gatcagggtc
     2401 acgaggaacg agtaggccag tacggcgccc gccgccacgg cctgacgcca cagctggtcg
     2461 gtgccgccgc cgtagaacaa gccgtccact ccggccggtg ccacggaggt ggccaggaag
     2521 ccgatcagca gcgtgcccac caggccgccg acgaggtgca cagcgaccac gtcgagcgag
     2581 tcgtcgaaac cgaaccggaa cttgatgctg accgctagcg cgcacaccgc gccggcgatg
     2641 gcacccacca caatcgcccc ggtcggggaa accgacgagc aggctggagt gatggcgacc
     2701 agaccagcca ccacgccgga ggcggcaccg aggctggtgg gccgcccgtc acggacccgc
     2761 tcgaccagca accagcccag catcgccgcg gcggtggcca ccatggtgtt gacgaaggcg
     2821 agacccgcga cgccgtttgc ggcgacggcc gatccggcgt tgaagccaaa ccatccgaac
     2881 cacagcagcc ccgcaccgag catcacaaag ggcaggttgt gcgggcgcat ggcctctcgc
     2941 ggccagccac ggcgcctgcc gagcaccagg cacaacgcca ggcccgcagc acccgcattg
     3001 atgtgcaccg ctgtcccacc ggcgaagtcg a
//
LOCUS       DATADN010000747         1958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1455179, whole genome shotgun sequence.
ACCESSION   DATADN010000747 DATADN010000000
VERSION     DATADN010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455179"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VHH53_09710"
     CDS             <1..152
                     /locus_tag="VHH53_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290448.1"
                     /translation="ELFTRRSRLADGHGIGLALARSLAEAEGGRLRLTRPAPPTFTLL
                     VPAQG"
     gene            complement(381..1025)
                     /locus_tag="VHH53_09715"
     CDS             complement(381..1025)
                     /locus_tag="VHH53_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2290449.1"
                     /translation="MTETVSTRAGATPRGVRMPRDERRAQLLAAATDVFVNNGYHATV
                     MDDIAEHAGVSKPVLYQHFPGKLELYLALLERHTDELVHRVRGAIEETHDNRQRVLNA
                     VQAYFDFVDGDGGAFRLVFESDLRNQPAVQAVVERAYTACVATIAQAVVADAGLGTGR
                     AWLVAVGVVGVSEHSARYWLSHQHEVPKDEAVALTASLAWKGLAGFPLQHNGPT"
     gene            1167..>1958
                     /locus_tag="VHH53_09720"
     CDS             1167..>1958
                     /locus_tag="VHH53_09720"
                     /inference="COORDINATES: protein motif:HMM:NF022794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3152 domain-containing protein"
                     /protein_id="HEX2290450.1"
                     /translation="MIRPTRADPAGRAPAGGRPRPGVPGQPAGRRWDDERISAPRLER
                     YSGSRIVNDEPLAASWDPRPEPVPRHGGPAGRSRVGRGSGRLARAVSIYGWRVYALPV
                     LVVLTVLAVLDAARSSTLPTEQPGTSDPAQQPLIPNPLAGPGFEAVKASAELPGGGPF
                     TVQGAGTWRVIPGAGAPFGIGQLFTYTIEIEDGVELEGGPAGFAATVDSTLNNPKSWI
                     GSGHYQFQRIDDSRAATLRISLTSQMTTRALCGFRIPFDASCWKPE"
BASE COUNT          288 a          663 c          671 g          336 t
ORIGIN      
        1 cggagctgtt cacccggcga tcgcgcctgg ccgacgggca cgggatcgga ctcgcgctcg
       61 cccgcagcct ggccgaggcg gaaggcggca ggctccggct gactcgcccc gctcccccaa
      121 ccttcacgtt gctggtgccc gcccaggggt gactggtgcc atcgacatga acccttggaa
      181 ggatgtcgtg actagctcgg cacgttggtg accgttggcg aggctcgcgt gacggtggcg
      241 catccgctgg ccggcatccg gctgaccgcg ggcacggtcg tgattttcgg accccgcgat
      301 gaggatgagc tggagatcgt gtccgggatc gcgggtgcga gtcgctcctt cgccgcgcac
      361 ggtcgaatcg cctggcctgg ttatgttgga ccgttatgtt gcagcgggaa gccggcgagg
      421 cctttccagg ccagtgacgc ggtcagcgcc acggcctcgt ccttgggtac ctcgtgctgg
      481 tgggacagcc agtaccgagc actgtgctcg ctgactccga cgactcccac cgcgaccagc
      541 caggcgcgcc cggtgcccag gcccgcatcg gccaccaccg cctgcgcgat ggtcgccaca
      601 caagcggtgt acgcccgttc cacgacggcc tgaacggcgg gttggttacg caagtcggac
      661 tcgaagacca gccgaaacgc cccaccgtcg ccatcgacga agtcgaagta ggcctggacc
      721 gcgttgagga ctcgctgccg gttgtcatga gtttcctcga tcgccccgcg aacgcggtgg
      781 accagctcgt cggtgtggcg ctccagcagc gccaggtaga gctcgagttt gccgggaaag
      841 tgctggtaga gcaccggctt gctgaccccg gcatgctcgg cgatgtcatc catgacggtg
      901 gcgtgatacc cgttgttgac gaagacatcg gtcgccgccg cgagcagttg agcccgccgc
      961 tcgtcccggg gcatccgcac tccgcggggc gtcgcgcccg cgcgggtcga taccgtctca
     1021 gtcatcgctc tcctttgccc tggctgattg ggttgaccct actagccggt atcccggtgc
     1081 cgccgctgga tcgcaggaca gattcagccg ctgcggcgtg gtgatatgcg cgaaacgcgc
     1141 gagagcggct gtgcaatcct tgcgcggtga tccgcccgac gcgagccgat cccgcgggcc
     1201 gggcccccgc tggcggtcgg ccccggccag gggtgcccgg ccagcctgcc ggccgtcgct
     1261 gggacgacga gcggatctca gcgccgcgcc tcgagcggta cagcggctcg cgcattgtca
     1321 acgacgagcc gttggcggcg tcgtgggacc cgcggcccga gccggtgccg cggcacggtg
     1381 gtccggcagg ccggagtcgg gtaggccgtg gttccggtcg cctggcgcgt gcggtgtcca
     1441 tctatggttg gcgggtctat gcgttgcccg tcctggtggt actcaccgtg ttggcggtac
     1501 ttgatgcggc caggtcatcg actctgccga ccgagcagcc cggcacgtct gatcccgcgc
     1561 agcagccgct gatacccaac ccgttggctg gcccaggttt cgaggcggtc aaggcatccg
     1621 ccgaactacc cggtggcggg ccgttcaccg tccagggcgc cggcacttgg cgggtgatac
     1681 ctggcgcggg tgcgccgttt gggatcggcc aactgttcac ctacaccatc gagatcgaag
     1741 acggggttga gctcgaaggc ggtcccgcag gttttgccgc caccgtggac agcacgctga
     1801 acaaccccaa gagctggatc ggcagcgggc actaccagtt ccaacgaatc gatgacagcc
     1861 gggcagcgac cctgcggatc agcctcacct cacagatgac cacccgtgca ctttgcggct
     1921 ttcgaatccc cttcgacgct tcttgctgga agcccgaa
//
LOCUS       DATADN010000748         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1005407, whole genome shotgun sequence.
ACCESSION   DATADN010000748 DATADN010000000
VERSION     DATADN010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005407"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1287
                     /locus_tag="VHH53_09725"
     CDS             178..1287
                     /locus_tag="VHH53_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290451.1"
                     /translation="MTPLLPTTVVGSYSVPEWLERLKTDYYRNRISRSHLGEIHDMAI
                     KAALKDQEEAGIDIVSDGELRRDNDIDYLLARIPGVQIPVTVKSFYYDYYDVVVADPL
                     PTDPSSLGLADDYRFTRQYTDRPVKFSFTGPFSLSHRIHNKAYSDSADLVRALAQVLN
                     AEARALAEAGAELLQIDEPFLAGYPEDVRVAVEAINIVTHGVDVTWGLHVCYGNRYAR
                     PSWEGHYDFLFPAVLDANVHQLILEFGRKGYEDLHVIQQLGWDRALGLGVVDVKTEQV
                     ESADLIAQRISKALETFPADKLIINPDCGLRNLPAAVARSKLAAMVEGTVAIRRTLPE
                     IQSDGHKSPGTPSGPESPVALSHVTPSEPTVEQGA"
     gene            1293..2531
                     /gene="metK"
                     /locus_tag="VHH53_09730"
     CDS             1293..2531
                     /gene="metK"
                     /locus_tag="VHH53_09730"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX2290452.1"
                     /translation="MTQLESIEYLFTSESVTEGHPDKMADQISDAVLDAALTMDPNSR
                     VACETLITTGMVVLAGEITTPEAVDYTQIVRDTICRIGYDNGIYGFDGHNCAVMTALD
                     KQSPDIAQGVDMAAERRGKLSEDELDSAGAGDQGMMFGYATNETPELMPMPIALAHRL
                     SQRLAKVRKDGTLPYLRPDGKTQVTVRYRNHVPIGVEKVLISTQHAEEVTSEQIKADL
                     WEHVVLAVLPADFYNEAELARNLFVNPTGRFVIGGPVGDAGLTGRKIIVDTYGGFSRH
                     GGGAFSGKDPSKVDRSAAYAARYVAKNVVAAGLADRVEVQVAYAIGVARPLSVFVETF
                     GTEQVPRTTIEKLVGEHFDLRPAAFRTYLGLHRPIYAKTAAYGHFGRDDPDFTWERTD
                     RAAELRESAGLPASTQPATV"
     gene            2552..>3370
                     /locus_tag="VHH53_09735"
     CDS             2552..>3370
                     /locus_tag="VHH53_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX2290453.1"
                     /translation="MGIIVTGSIATDYLMVFPGKFVEQILPNQLENLSVSFLVEDLAI
                     HRGGAAANIAFNLGCLGLAPALVGAVGADFAEYGQWLSAHGVDISAVHVSQTKHTARF
                     QCTTDSDQNQIASFYAGAMSESRDIELKPIVARLGGTDLVVVGPSDPAAMLRHTQECR
                     DLGYPFAADPSQQLASLEGDQITQLVEGAAYLFTNSYERGLLERKTGWSEEDILSRVD
                     VSVMTRGAKGVTVQRPGEPAISVTPPQELVKADPTGVGDALRAGFLAGVQWGVTD"
BASE COUNT          613 a         1087 c         1113 g          557 t
ORIGIN      
        1 cgcggccccg gcaagtacga cggctcggtc gtgaactggc tcaacggtcc gctgctggcg
       61 cacatcgggc gccgcaaggg ctttgaggtg gactaccagc ggttcgcgca ccggaccggc
      121 accaacatcg tcaccatgac agcccaagta agggattgat acaggtgagg gactgatatg
      181 acccccctgc tgcccaccac cgtcgtcggg tcgtactcgg tccccgaatg gctcgagcgg
      241 ctcaagaccg actactaccg caaccggatc agccgcagcc atctcggcga gatccacgac
      301 atggcgatca aggcggcgct caaggatcag gaggaggctg gaatcgacat cgtctccgac
      361 ggagaactgc gccgggacaa cgacatcgac tacctgctgg cccggatacc gggagtgcag
      421 atcccggtta ccgtcaagtc gttctattac gactactacg acgtcgtcgt cgccgatccg
      481 ctgccgaccg acccgtcgtc gctaggcctc gccgacgact atcgcttcac ccgtcagtac
      541 acggaccgtc cggtcaagtt ctcctttacc ggacctttct cgctgtccca ccggatccac
      601 aacaaggcgt attcggattc cgcagacctg gttcgagcgt tggcccaggt gctcaacgcc
      661 gaggcgcggg cgctggccga ggcgggtgca gagctgctac agatcgacga accgttcctg
      721 gccggctatc ccgaagacgt gcgggtggcg gtcgaggcga tcaacatcgt gacccacggc
      781 gtcgacgtca cgtgggggct gcatgtctgc tacggcaacc gctacgctcg accgtcatgg
      841 gaagggcact acgacttcct cttccccgcg gtgctcgacg cgaacgtgca ccaactcatc
      901 ctcgagttcg gccgcaaagg ttacgaggat ctgcatgtga tccagcagct cggttgggac
      961 cgcgcgctcg ggctcggcgt ggtggacgtc aagaccgaac aggtcgagtc ggcggatctc
     1021 atcgcccagc gcatcagcaa ggcgttggag accttcccgg ccgacaagtt gatcatcaat
     1081 ccggactgtg ggctgcgcaa tcttcccgcc gctgtcgcga ggtcgaagct ggcagcgatg
     1141 gttgaaggga cagtcgcgat ccgccgcacg ctgcccgaga tccagtccga tggacacaaa
     1201 tcccccggaa cccccagtgg cccggaatcc ccagtggcac tgtcacacgt gacaccgtcg
     1261 gagccgaccg tcgagcaagg agcgtgatct gcgtgaccca gctggagagt atcgagtacc
     1321 tgttcacctc cgagtcggtg accgaaggcc acccggacaa gatggccgat cagatctccg
     1381 acgctgtgct ggacgcggcg ttgacgatgg atccgaactc gcgggtggcc tgcgagaccc
     1441 tgatcactac cggcatggtg gtgctggcgg gcgagatcac cacaccggag gcggttgact
     1501 acacccagat cgtgcgcgac acgatctgcc gcatcggcta tgacaatggg atctacggtt
     1561 tcgacggcca caactgcgcg gtgatgaccg cattggacaa gcagtcgccc gacatcgccc
     1621 agggggttga catggctgcg gagcggcgcg ggaagctgtc cgaggacgaa ctggacagtg
     1681 ccggtgcggg cgaccaggga atgatgttcg ggtacgccac gaatgagacc cccgagctga
     1741 tgccgatgcc gatcgcgctg gcccaccggc tgtcccagcg gctggcgaag gtgcgcaagg
     1801 acggcaccct gccctacctg cgcccggacg gcaagaccca ggtgaccgtc cggtaccgga
     1861 atcatgtgcc gatcggggtg gagaaggtgc tgatctctac ccagcacgcc gaggaagtca
     1921 cctccgagca gatcaaggcg gacctatggg agcacgtggt gcttgcagtg ctcccggccg
     1981 acttctacaa cgaggccgag ctggcgcgca atctgttcgt caacccgacc gggcggttcg
     2041 tgatcggcgg gccggtcggt gatgccgggc tcaccggccg gaagatcatc gtggacacct
     2101 acggcgggtt ctcccggcac ggtggcggcg cgttctccgg taaggacccg tccaaggtgg
     2161 accgttccgc ggcgtacgcg gcccgctacg tggccaagaa cgtcgtggcc gcgggtctgg
     2221 ccgaccgggt cgaggtgcag gtggcctacg cgatcggcgt cgcccggccg ctgtcagtgt
     2281 tcgtggagac cttcggcacc gagcaggtac ctcgcacgac catcgagaag ctggtgggag
     2341 agcacttcga tctgcgcccg gcggccttcc gtacttacct ggggctgcac cggccgatct
     2401 atgctaagac cgccgcctac ggccacttcg gacgcgacga tccggacttc acgtgggagc
     2461 gaaccgacag ggcggcggag ctgcgggagt ccgccgggct acctgccagc acgcagccgg
     2521 caaccgttta accgggctca gggaggcttt ggtgggcatc atcgtcacag ggtcaatcgc
     2581 caccgactac ctgatggtct tcccgggcaa gttcgtcgag cagatcctgc ctaatcagct
     2641 ggaaaacctc tcggtgtcct tcctggtgga agatttggcg atccaccgtg gcggcgcggc
     2701 cgccaacatc gcgttcaacc tggggtgcct cgggctggcg ccggcgttgg tgggcgcggt
     2761 cggtgccgac ttcgccgagt acgggcaatg gctgtcggcg cacggggtcg acatcagcgc
     2821 ggtgcacgtc tcgcagacca agcacactgc ccggttccag tgcaccaccg actccgacca
     2881 aaaccagata gcctcgttct acgccggtgc gatgagcgaa tcccgcgata tcgagttgaa
     2941 gccgatcgtg gcccgcttgg gcgggaccga cctcgtggtg gtggggccca gcgacccggc
     3001 agcgatgctg cggcacactc aagagtgccg ggatctgggt tatccgtttg ccgccgaccc
     3061 ctcccagcag ctcgcctcgc tggaaggcga ccagatcacg cagctggtcg aaggggcggc
     3121 ctacctgttc accaacagct acgagcgagg actgctggaa cgcaagaccg gctggtccga
     3181 ggaggacatc ctgtcgcggg tggacgtctc tgtgatgacc cgcggcgcca agggtgtgac
     3241 cgtgcagcgg ccgggcgaac cagccatctc ggtgacaccg ccgcaggagc tggtcaaggc
     3301 ggatcccacc ggcgtcggcg atgcgttacg agccggcttc ttggccgggg tccaatgggg
     3361 tgtgactgac
//
LOCUS       DATADN010000749         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1155843, whole genome shotgun sequence.
ACCESSION   DATADN010000749 DATADN010000000
VERSION     DATADN010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155843"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VHH53_09740"
     CDS             <1..757
                     /locus_tag="VHH53_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290454.1"
                     /translation="PTTQQVFTRPALPEKTTTPAAPFTKVDAAAGPPPTEDDLDGSPG
                     RRKLLAGVLAVAILAGGGLITALFNDGDITGGKAISGNAARPSSTSDDPGQSLQPLPA
                     PSAPSSATQGLQTQPTPSGSRSTTQAAIPTGPVAPGGSQSRPAQLQVITDYYALVPDN
                     LPAAWARLTADYQQNHAGGFAGYQDFWNQVQQVTVTDVSVQQGDAVDATVEYVFKDGR
                     VIEERTSYGLLDEDGVWKIDSSTVRSSQAKQVN"
     gene            917..1150
                     /locus_tag="VHH53_09745"
     CDS             917..1150
                     /locus_tag="VHH53_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX2290455.1"
                     /translation="MSSFEKAKGKTEQLIGKAEKKVGQMLGHEDLENAGERDQLTGEV
                     KEANHDLRDDSVTGTADDAGAADDTSNQGHEAR"
     gene            complement(1310..1576)
                     /locus_tag="VHH53_09750"
     CDS             complement(1310..1576)
                     /locus_tag="VHH53_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290456.1"
                     /translation="MQHSAWEHGDLPLFVTATRDSPGVVIRCPATGRVYSWALEPHPD
                     GCRVRVRVEVPDGQYEQLRLSRETMLATLPQLAATAERPSTEPA"
BASE COUNT          367 a          599 c          593 g          308 t
ORIGIN      
        1 ccccacaacg cagcaggtgt tcacccggcc ggccctaccc gagaagacga ccaccccggc
       61 ggcgcctttc acgaaggtgg acgccgcagc cggtccgccg ccgaccgagg atgacctcga
      121 cggatcgcca gggcgccgca aactcctggc cggtgtctta gcggtcgcga tccttgctgg
      181 cggtggtctg atcaccgcgc tcttcaacga tggcgatatc accggcggca aggccatcag
      241 cggcaacgcc gcccggccct cgagcacctc cgacgatccg gggcaaagcc tccaacccct
      301 tccggcccca tcagcaccga gcagcgcaac gcaaggtctc caaacccagc caactccgtc
      361 aggttcgagg agcacgacgc aggcagccat cccgaccggt ccggtggccc cgggcggctc
      421 acagagcagg cctgcgcagc tgcaggtgat taccgactat tacgcgctag tgcctgacaa
      481 cctgccggct gcgtgggcac ggcttaccgc ggactatcag cagaatcatg ccgggggctt
      541 cgcgggatat caggacttct ggaaccaggt gcaacaagtc acggtcaccg acgtctctgt
      601 gcagcaggga gatgccgtgg acgcaacagt cgagtacgtc ttcaaagacg gccgggtcat
      661 cgaggagcgt accagttatg gactgctcga cgaggacggt gtgtggaaaa ttgacagttc
      721 cacggtgcgc agtagccagg ctaagcaggt caactgacaa cgcagcgaca agccgttacg
      781 cttcactgta ctggcgccta tccgtacatc cgatagggtg gtcgaacgcc gctgacgtat
      841 gtcagaggct attttgggtc ggtccgatca ccaggtacac gcgtacaggt ggtccctgat
      901 cataagggag aaaatcatga gcagttttga aaaggcgaag ggcaagaccg agcagcttat
      961 cggtaaggcg gaaaagaagg tcggtcagat gttgggtcac gaggaccttg agaacgccgg
     1021 ggagcgtgat caattgaccg gtgaggtcaa ggaagccaac cacgatctac gcgacgacag
     1081 cgtgaccggg actgccgatg atgccggtgc tgctgacgac acctcgaacc aaggtcacga
     1141 ggctcggtaa gtagtggctg tgggacatcg ccacaccaag ccccctatcg cggtgcgtgg
     1201 taacggtgcc tcgtgacgtc ggtgagggaa aatgcggatc gacgtcgaga tccttgcctg
     1261 gatgcgccac cgcccggagg ctgaacacgg cgacttcccc gggccaccgt cacgccggtt
     1321 cggttgaagg cctttcggcc gtggccgcca actggggcaa cgtggcgagc atggtctcgc
     1381 ggctcaggcg tagctgttcg tactggccgt ctgggacttc cactcgcact cgcacccggc
     1441 atccgtcggg atgtggctcg agcgcccagg agtagacgcg ccccgtcgcc gggcaacgga
     1501 tcacgacgcc gggggagtcc cgcgtcgccg tgacaaacag cggcaggtcg ccgtgctccc
     1561 atgcgctgtg ctgcacgacg gcaccatcgg cggtggctgt gatcgggtga tgcccaccca
     1621 ccattcgggg aaccgggtgg ggtcatggag aagcttccac acctgcagcg ccggagcccg
     1681 gcacactacg gtttcctcga agtcgatcac ctcacacccc tttcgttgtg gtcggtatcg
     1741 gtatgtcacc caacgcgcag cggtagttct cgacacccag cgctgtggcc agcctcgccg
     1801 cctcctgctt ggccgcgatc gcttcggcag tatgcccagc tgcggcgagc agtccagccc
     1861 gttccac
//
LOCUS       DATADN010000750         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_77316, whole genome shotgun sequence.
ACCESSION   DATADN010000750 DATADN010000000
VERSION     DATADN010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77316"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /gene="mtrB"
                     /locus_tag="VHH53_09755"
     CDS             <1..1250
                     /gene="mtrB"
                     /locus_tag="VHH53_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HEX2290457.1"
                     /translation="GTGQKIGPLDDVPAVLQSAVERGVLAHKITTVQRDSTAVTMLAV
                     GMPVASAGRTMQLYLLFPLTAEQRTLHLVQSTLVVGGLVLLGLIAGIASLVTHQVVIP
                     VRQAAEVAERFADGHLSERIPVVGSDDMARLGTSFNEMAASIQRQIRQLEEFGELQRG
                     FTSDVSHELRTPLTTVRMAADVLYDSRDMLHPVLHRSTELLVNELDRFEALLGDLLEI
                     SRLDAGVAELHSETLDVRVTVENAVAAVRAVADQAGTPLELELPATEVLAEIDPRRVE
                     RIVRNLLTNALDHGEGHPVEVVVAADSDAVAVLVCDHGVGLHPDQHELVFTRFWRGDP
                     SRDRRTGGTGLGLSISLEDARLHGGWLQAWGEPGRGSAFRLTLPRSRGIELSGSPLLL
                     GPRNVPSALPATGSPASAGPGTR"
     gene            1247..>1657
                     /locus_tag="VHH53_09760"
     CDS             1247..>1657
                     /locus_tag="VHH53_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290458.1"
                     /translation="MMPVTLRRAAVLAAVFASLVGCAAVPGTSDMQVVRSMPVGSAGA
                     PLAGPEQGVDPFRLVRDFIEATGSPANGHAAARAFLSREAATSWDDRAGLIVIEDAPG
                     ATPQAGPEDGVRRIRVGGPRLGVLAADGSFTPAAG"
BASE COUNT          228 a          561 c          601 g          267 t
ORIGIN      
        1 ccggtacagg gcagaagatc ggcccgctcg acgacgtccc cgcggtgctg cagtccgccg
       61 tggaacgcgg cgtgctcgcc cacaagatca ccacggtgca acgggactca accgcggtga
      121 ccatgctggc ggtcggcatg ccggtcgcca gcgcgggccg caccatgcag ctttacctgc
      181 tgtttccgct caccgcagag cagcgcacgc tgcacctggt gcagtccact ctggtggtcg
      241 gcggcttggt gctgctgggg ctcatcgcgg gaatcgccag cctggtgacc caccaggtgg
      301 tgatccccgt ccggcaggct gctgaagtgg ccgaacgctt cgccgatggt catctctccg
      361 agcgcattcc ggtggtcgga agcgatgaca tggcccggct gggcacgtcg ttcaacgaga
      421 tggccgcgag catccagcgg cagattcgtc agctcgagga gttcggtgag ctgcagcgcg
      481 gtttcacctc cgacgtctcc cacgagctgc gcaccccgct gaccaccgtg cggatggctg
      541 ccgatgtgct gtatgactcg cgggacatgc tgcacccggt gctgcatcgg tccaccgagc
      601 tgctggtcaa cgagctggat cgattcgagg cgctgctcgg cgacctgctg gagatctccc
      661 ggctcgacgc cggggtcgcc gagctgcaca gcgagacgct cgacgtgcgc gtcaccgtgg
      721 agaatgcggt ggccgccgtg cgtgcggtcg ccgaccaggc gggcaccccg ctggaactgg
      781 agctcccggc cacggaggtg ctagccgaga tcgatccgcg ccgggtggag cggatcgtgc
      841 gcaatctgct caccaacgcg ctggaccatg gggagggtca tccggtcgag gtggtggtcg
      901 ccgcggactc cgacgcggtc gccgtgctgg tgtgcgacca cggcgttggg ctgcacccag
      961 atcagcacga gctggttttc accaggttct ggcgcggtga tccgtcgcgt gaccggcgca
     1021 ccgggggcac tggtctggga ctatcgatca gcctggagga cgctcggctg cacgggggct
     1081 ggttgcaggc ctggggcgaa cccgggcggg gcagcgcttt ccggctcacc ttgccccgca
     1141 gccggggcat cgagctcagc ggcagcccgc tactgctggg acccagaaac gttcccagcg
     1201 ccctccccgc gacaggctcc ccagcgtcgg ccggacccgg tacccgatga tgccagtgac
     1261 gttacggcgc gccgccgtcc tcgccgccgt gttcgcctcg ctggtgggct gtgctgccgt
     1321 cccgggcacc tccgacatgc aagtggtgcg ctcgatgccg gtgggctcgg caggggctcc
     1381 gctggccgga ccggaacagg gcgtggatcc cttccggctg gtgcgagatt ttatcgaggc
     1441 gaccggtagc ccggcgaacg ggcatgccgc cgcgcgtgcc ttcctgtccc gggaggcggc
     1501 gacgagttgg gacgatcgcg ccggactgat cgtgatcgag gacgctcctg gcgctacgcc
     1561 gcaggccggc ccggaagacg gtgtccgccg tatccgggtc gggggcccgc ggctgggcgt
     1621 gctcgccgcg gacggcagct tcacgccggc agcaggg
//
LOCUS       DATADN010000751         4373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_835924, whole genome shotgun sequence.
ACCESSION   DATADN010000751 DATADN010000000
VERSION     DATADN010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835924"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..834)
                     /locus_tag="VHH53_09765"
     CDS             complement(367..834)
                     /locus_tag="VHH53_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2290459.1"
                     /translation="MIVDNGNTDQATALKNSRWALLKNPPDLTSDQRTTVAAIAKTNQ
                     PLYRAYLLKEQLREVFATKGATGRQLLAGWLSWATRSRIPEFAALAKTIKRFLPLIHN
                     TLNHGLGNALSEATNTHLRLLTRRAYGYHSAEALIAMATLTRGGLCPPLPGRP"
     gene            1010..2625
                     /gene="istA"
                     /locus_tag="VHH53_09770"
                     /pseudo
     CDS             1010..2625
                     /gene="istA"
                     /locus_tag="VHH53_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_176223647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            2622..2819
                     /locus_tag="VHH53_09775"
     CDS             2622..2819
                     /locus_tag="VHH53_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290460.1"
                     /translation="MTAAPTNRAVPDRASSDTAALVPTATTGATTGVAATGSPAAIMS
                     DPAAVNAALTPSPAALSPATP"
     gene            2902..3177
                     /locus_tag="VHH53_09780"
     CDS             2902..3177
                     /locus_tag="VHH53_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290461.1"
                     /translation="MCGSCQQQRSTLGKGQQPTPITITEPIDLLAWIAQAHHNLDTAE
                     RVLAAAVARARQHRHPWSAIGNTLHISRQAAQQRFGPTAATAEGRTP"
     gene            3240..3952
                     /gene="istB"
                     /locus_tag="VHH53_09785"
                     /pseudo
     CDS             3240..3952
                     /gene="istB"
                     /locus_tag="VHH53_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
BASE COUNT          727 a         1530 c         1427 g          689 t
ORIGIN      
        1 aggcatcgca agcatctagg gttgggaccc cgacggctgg tcattctgtg cgctggtgtt
       61 cctcaccggc gcgctcggga tggctggtga tgtgccgtct gcacgccaag cgcttgtgga
      121 gatgacggcc gtgcggcatc ccgcctaagc acgttccgcg gatattggcc atcgcagttc
      181 ggctcgatgt atttgagtga gcgtatcggg caacttattg ggttgccctt gacagggtaa
      241 ccacagcaaa tttgaaccac gagggtttat tcaaaccccc gactgaagac acgcgtgcag
      301 aatcgttgga ggggcacgcc gccatactcg catgcggggc ctactgctgt ttccgtgggt
      361 cgggtttcat ggtcgtccgg gtaggggtgg gcagagtccg cctcgggtga gggtggccat
      421 ggcgatgagt gcttcggcgc tgtggtagcc gtaagcgcga cgggtcagca gacgcagatg
      481 agtgttcgtg gcctcggata aggcgtttcc tagaccgtgg ttgagggtgt tgtggatcag
      541 cggaagaaac cgtttgatgg ttttggctag tgccgcgaac tcggggatgc gggatcgggt
      601 ggcccaggac agccagccgg cgagcagctg tctgcctgtg gctcccttgg tcgcgaagac
      661 ctcgcggagt tgctccttga gtaggtaggc gcggtagagc ggctggttgg tcttggcgat
      721 ggcggccacg gtggtgcgct ggtcgctggt gaggtctggt gggtttttca gcagtgccca
      781 tcggctgttt ttgagcgctg tggcctggtc ggtgttgccg ttgtcaacga tcaagttatt
      841 cctccagcgc gaggggttga cgatcacgta attcccccac ccggcgatca cggaattccc
      901 ccaggggaga cgtgcgtgtc ggtaccgggt ctggtcctca gtagacgcaa gcctgcgcct
      961 cgttgccgag cagcgaggtg gagccactgt ggcgaggagg accttcgaag tgatcgacat
     1021 tgtggagatt ttgatccact ggtatgcggg caggtcgcag catgaactgg cggccagcct
     1081 gggtgtggat cgcaagacgc agcgcaagta caccggtccg gcccgtgcgg ccgggatcga
     1141 gcccggcggc ccgttgatga gcgaggctga ctggcgcgcg ctggtggcgg agtggttccc
     1201 ggaactgtgc gactccgggc tgcggcaggt gtcctggccg cggatcgagc cgcaccggga
     1261 ctacatcgcc gcccagctca aggccggggt gaccgtcgca atgacgcatc agcggctcgc
     1321 cgacgagcac gggttggacg cgtcggtggc cagcgtgcgc cgctgggtgc gggccaacct
     1381 gcccgaggag gtgcggcgcg ctcaggtgac ggtgctgcgc gagtgcccgc cgcccggttc
     1441 ggaggcgcag atcgactatg ggcgcctggg gatgtggctg gacccgcgcg ccgggcgacg
     1501 gcgcacggtg caggcgtttg tgatggtgct gtgctgttcg cggcacatgt tcgtgcgccc
     1561 gacgctggtg atggaccagg ccgaatggac cgccgcgcat gtggaggcgt tcgcgttctt
     1621 cggtggggtg cccgcgcggc tggtcccgga caatctcaag accggggtcg aacggcccga
     1681 cctgtatgac ccgaaggtca accggtcgta tgccgagctg gccgcgcatt acggcaccct
     1741 ggtcgacccc gcccgcgcgc tcaagccgaa ggacaaggcg caggtggaac ggccgatgcc
     1801 ttatgttcgg gactcgttct ggcggggccg ggagttcacc tcgctggagg cgatgcgcgc
     1861 cgatgcgctg cgctggagca ccgaggtggc cggtgcgcgg gcgtgccggc cgctggacgg
     1921 ggctgccccg gcggcggtgt tcgccgcagt cgaggcgccg acgcttcagc cgttgccgtc
     1981 cgcgccgttt gtgctggcca gctgggcttc ggcgaagatc ggcccggacc tccatgccaa
     2041 ggtcggcgcc accttgtact cgttgccgtg gcgctacctc ggtgagcggg tcgacgcccg
     2101 ttccaccacc tcgatggtgc agttcttccg ccacggtgaa ctgatcaaga cccatccccg
     2161 taaggagcgc gggaagcaga ccgacttcgg ggactaccca ccggagaaga tcgccttcca
     2221 catgcgcacc ccggcctggt gtcggcgccg ggccgccgag atcggtcccg ccgccaccga
     2281 ggtgatcggc gagttgctgg ccgacaacgc gctgttccgg ctgcgcgccg cccagggcgt
     2341 gctcggcctg gccgacaaac acgatcccgg gcggctggag gcggcgtgcg cgaaggccac
     2401 cgtggccggg gacccgtcct accgcacggt gaaggcatcc tcgccgccgg caccgagacc
     2461 accacggctg cccggcccgg cggggacgcc ggcgccccgg cgcacttgca cggacccgac
     2521 cagctcttcg ctgaccccat cgccaccgtc atccccctgc acaccgccac cggccccgca
     2581 cccgcgggcg cgacccagct gaccagcacc gaggacgcct cgtgaccgcc gcgccgacca
     2641 accgcgccgt cccggaccgg gccagcagcg acaccgcggc gctggtcccc accgccacca
     2701 caggcgccac cacaggcgtc gccgcaaccg ggagccccgc cgccatcatg agcgaccccg
     2761 ccgccgtcaa tgcggccttg acaccatcgc cggccgcgct gtcaccggcc acgccatgac
     2821 catccgctgc tactacgcca ccgacctcgc ccccgcccgc actgcgggct cctggccgcc
     2881 gtccgctacg gccgcaccgc tttgtgcggc tcgtgtcagc aacagcgctc caccctgggc
     2941 aaaggtcaac aaccgacccc gatcaccatc accgaaccga tcgacctgct cgcctggatc
     3001 gcccaggcac accacaacct agacaccgcc gaacgggtcc tggccgccgc ggtcgcccgc
     3061 gcccgtcaac accgacaccc ctggtcagcc ataggcaaca ccctgcacat cagccgccag
     3121 gcagcccagc aacgcttcgg acccacagcc gcgaccgccg aagggcgtac cccatgagca
     3181 caccgaccat cgacaacaac gcactaaccg cgtcgctgcg cgcactgaag ctgggcggga
     3241 tgctgcagac cctcgacgcc cgcctagctc aggcccgcgc cggggagctc gggcacctgg
     3301 agttcctgca ggtcctatgc cacgacgaga tctcccgacg cgagaccacc gccatcaccc
     3361 aacgcacccg acgggcccgg ttcgaggaaa ccctcaccct ggaagggttc gacttcgccg
     3421 ccgccccgaa actcccagcc gctcagatcc gtgacctggc cgcgctgcgc tggctccagg
     3481 ccggcgagtc ggtgatcctc tacggcccgg tcggggtggg caagacacac atcgcccaag
     3541 ccctcgcgca cctggccatc cgggcggcgc cgaagcccgg ttcagcaaaa ccagccgcgc
     3601 cctggccgag ctcgccggcg gccacgccga cggcacctgg acccgccgcc tacgcgaact
     3661 cccccgaccc gccgtcctgg tcctcgacga cttcgccatg cgggaactca cccccgccca
     3721 agccgacgac ctctacgaac tgatcaacga acgcaccggc cgatccctga tcctgacctc
     3781 gaaccgctcc ccggtggact ggtacccgct gttccccaac cccgtcgtcg ccgagtccct
     3841 actcgaccga ctgatcaaca acagccacca gatcctcatg aacggagcca gctaccgacc
     3901 ccacaaacga cccgggcgcg tcgcccccca gaacaccaag acaacccact aaactgccca
     3961 ccacggacca gacccggggg aattacctga tcccagcccc gggggaatta cctgatcgtt
     4021 cacactagct cagccaacac ggtggcgact gcggccgcct ggtcaatgag gtggggcaaa
     4081 cccatcaaaa ccacccaggg aaaagatcat tatgtcgcgg aatcagcttc tccgactcca
     4141 cttctggcgg acagcgtaga ccccctgtag ggcatggcag cggtgactat ttcatgtcgg
     4201 tcgaggtctt ttgtgactag ccacacccac taacgtcact gccgcccaaa tggacgcact
     4261 cagccaagaa cccagtgact ggctgttctg catggtcagg ctctgcgagt tgtggagggg
     4321 ccagggttcg cccggacagc tttgagtggc ggccagcgcg gagcgcctcc ggc
//
LOCUS       DATADN010000752         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_696553, whole genome shotgun sequence.
ACCESSION   DATADN010000752 DATADN010000000
VERSION     DATADN010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696553"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1332
                     /gene="manA"
                     /locus_tag="VHH53_09790"
     CDS             73..1332
                     /gene="manA"
                     /locus_tag="VHH53_09790"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEX2290462.1"
                     /translation="MELLRNVVRPYSWGSRTAIAELLGGPVPAPHPQAELWLGAHPAG
                     PSVLLHADGAQTSLLEAVHTNPDHYLGPHCARRWGNRLPFLLKVLAAEEPLSLQAHPS
                     AQQAAEGFFREEARGIPRDASERNYSDPHHKPELICALTDFHALAGFRDPLRTVELLT
                     ELDTPLLAPYRVMLDGEPNADGLRALFTTWITMPQPAAKELLPRIVDACVALLRRDGV
                     GGAFPRSHGSDFRSVCRVILELTEAHPDDVGVLAALLLNHVVLAPGEAMYLPAGKLHT
                     YLRGTAVEISTNSDNVLRGGLTAKHIDVPELLRVLNFSHGELPVQRGKPVGPHETAYH
                     APAEEFRLTRLEWADGDTTPVWLQSPWPQILLCTQGSAELRASDGSSVTVRRGGSVWL
                     AAADPAVEVCPETGPVQLFRAQPGLTT"
     gene            1468..>1613
                     /locus_tag="VHH53_09795"
     CDS             1468..>1613
                     /locus_tag="VHH53_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX2290463.1"
                     /translation="MSAGGGTKAIVAALLANAGIAVSKFIGYGITGSSSMLAEAVHSV
                     ADTST"
BASE COUNT          244 a          552 c          552 g          265 t
ORIGIN      
        1 gccggctggc gggccgcttg ctcatccgac cgctgactcg gctgggccgg gccggccggc
       61 aaaggagggt ctgtggagct gttgcgcaac gtggttcggc cgtactcctg gggttcccgg
      121 actgcgatcg cggagctgct cggcggccca gtgcccgccc cacatccaca ggcggaactg
      181 tggctcggcg cgcatccggc gggtccatcg gtgctgctgc acgcagacgg cgcgcagacc
      241 tccctcttgg aggctgtgca taccaacccg gatcactacc tcgggccgca ctgcgcgcgc
      301 cgctggggca accgattgcc tttcctgctc aaggtgctcg ccgccgaaga accactgagc
      361 ctgcaggccc atccgtctgc gcagcaggct gccgagggat tcttccgtga ggaggctcgg
      421 ggaatccccc gggacgcctc ggaacgcaac tactccgacc cgcaccacaa acccgagctg
      481 atctgcgcgc tgaccgactt ccacgcgctc gcgggtttcc gggacccgct gcgtaccgtg
      541 gagttgctca ccgagctgga cacacccctg ctcgcgccct accgcgtgat gctcgatggt
      601 gagcccaatg ccgatggact gcgcgccctg ttcaccacct ggatcacgat gccccagccc
      661 gcggccaaag aactactgcc ccgcatcgtg gacgcctgcg tcgcgctgct gcgccgcgac
      721 ggggttggtg gcgcctttcc gcgctctcac ggcagcgatt tccgcagcgt gtgccgggtg
      781 attctggagc tgaccgaggc gcatccggac gacgtcgggg tactggcggc cttgctgctc
      841 aaccacgtcg tgctggcgcc gggtgaagcg atgtacctgc cggccggcaa gctgcacacc
      901 tacctgcggg gcactgcggt ggagatcagc acgaattcgg acaacgtgct gcgcggcggt
      961 ttgactgcca agcacatcga cgtccccgaa ctgctgcggg tgctgaactt ctcccacggc
     1021 gaattgccgg tgcagcgcgg taagccggtg gggccgcacg agaccgccta ccacgccccc
     1081 gccgaggagt tccggctgac ccggttggaa tgggcggacg gcgacacaac cccggtgtgg
     1141 ctgcagagcc cgtggccgca gatcttgctg tgcacccaag ggtcggcgga gttgcgcgcc
     1201 agcgacggca gcagcgtcac ggtgcgccgt ggggggtcgg tatggctggc cgccgcggac
     1261 ccggcggtcg aggtgtgccc ggaaaccggc ccagtccagc tgttccgcgc acagcccgga
     1321 ctcaccacct gatccggcag ccgctcccgg cctcgattcg gctccgaata acgaccttta
     1381 aggctgttca aaccgccatt cggttccgaa ttccggcagc taggctcacc ggtgcaagtg
     1441 agacgggcag cgggaggagg cgctggggtg tcagcgggtg gtgggaccaa ggcgatcgtc
     1501 gcggcgttgc tggccaatgc gggtatcgcg gtgtcgaagt tcattggata cgggatcacc
     1561 ggctcgtcgt cgatgctcgc cgaggcggtg cactcggtgg ccgacacgtc cac
//
LOCUS       DATADN010000753         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_549969, whole genome shotgun sequence.
ACCESSION   DATADN010000753 DATADN010000000
VERSION     DATADN010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549969"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VHH53_09800"
     CDS             <1..479
                     /locus_tag="VHH53_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX2290464.1"
                     /translation="GVRIVVLTNAAGAIAERLEVGQPVLISDHLNLTAASPLVGPQFV
                     DLTDTYSARLRSMAREIDPSLVEGVYAGVPGPHFETPAEIRMLRVLGADLVGMSTVLE
                     AIATRAEGAEVLGISLVTNLAAGLDGAVMDHREMLATAHSAAARMGKLLRELISRT"
     gene            complement(597..1067)
                     /locus_tag="VHH53_09805"
     CDS             complement(597..1067)
                     /locus_tag="VHH53_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290465.1"
                     /translation="MTRKGVPQGRILHVLLPRGAGIVERLFPGYARELTAAGAVSLRV
                     PTDALVLTPAGWLDRRAIGWPLLSASRPLFEWAVRRRRRELPGVRILDRHDVTSLVTS
                     HDGRQIAGVCAHLVLHDYRWHYLARIWRTPVLGEARKPSLRPKSSSSMTAAIGR"
     gene            1316..1417
                     /locus_tag="VHH53_09810"
                     /pseudo
     CDS             1316..1417
                     /locus_tag="VHH53_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1593..>1798
                     /locus_tag="VHH53_09815"
     CDS             1593..>1798
                     /locus_tag="VHH53_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290466.1"
                     /translation="MRTELSLPVNPSRRIRLSGCVSLLALLAGLGGCSSMFSVDEPAW
                     CVMMDCWLAVEPVKAGSSMLNMLL"
BASE COUNT          303 a          615 c          582 g          298 t
ORIGIN      
        1 cgggcgtccg gatagtggtg ctcaccaacg ccgctggcgc tatcgccgag aggctcgagg
       61 tcgggcaacc ggtgctgatc tctgaccacc tcaacctcac cgcagcctcc cccctggtcg
      121 gcccgcagtt cgtcgatctc actgacacct actccgcccg gctgcgctcg atggcccgcg
      181 agatcgaccc gagcctggta gagggcgtct acgccggggt gcctggcccg cacttcgaaa
      241 ctccagccga gattcgcatg ttgcgcgtgc tgggagctga cctggtcggg atgtccaccg
      301 tgctggaagc catcgcgacc cgcgctgagg gtgccgaggt gctgggaatc tcactggtca
      361 ccaacctcgc cgccggcctt gacggggcgg tgatggacca ccgggagatg ctcgccaccg
      421 cccacagcgc tgcggcgcga atgggcaagc tactgcggga gctgatcagc cggacctgat
      481 ggtgcagcga aggaccccat cccgcccaag gccgtgtcac gcgtcgcgta cggcgccgcc
      541 gatctgagcg ctcaggaacc ggagcacggg ctgctcgacg gcgactggat gatcgatcat
      601 cggccaatgg ccgctgtcat cgaggatgac gacttcggcc ttagggaagg tttccgggct
      661 tcaccgagca ccggggtccg ccagatccgg gccaggtagt gccagcggta gtcgtgcagc
      721 accaggtggg cacacactcc ggcgatctgc cggccgtcat gtgaggtgac cagcgacgtc
      781 acgtcatgac ggtcgaggat ccgcacaccc ggcagttccc gccgccgccg gcgaactgcc
      841 cactcgaaca gcggccggct cgccgacagc agtggccacc cgatggcccg ccggtccaac
      901 cagccggcag gggtcaggac gagagcgtcg gtggggacgc gtaacgacac tgccccggct
      961 gccgtcagct cgcgcgcata gccggggaac agccgctcaa cgatccccgc gcctcgaggc
     1021 agcagcacat gcagtatgcg tccctgcgga acgcccttgc gcgtcacaac gctgtccgtc
     1081 agcgcgtcgc gctcgacgag cgtcgcctac tcacagtgat tggcgagcac ccgggcggcc
     1141 agcaggccag ccatgctgcc accgatgacg acggcgtgac cgggtccggt cgactcagcg
     1201 caggcgggcg agggatggat ctccaaggat ccgttgccgt caggcgtacg gctcaggagg
     1261 ttctcctcga tatttcaaac actgttataa aacaccgttg cgatacgcta gtcaagtgcc
     1321 acgacctaaa ttgcacgacg atgcgctgcg gatccggctg ctcgataccg caggtgcatt
     1381 gctcacctcc gagggcccgg acgcactgag cctgcgcccc ggggatgcag cgcctcatcc
     1441 tcttccagcg catcgggcac aacgtcgaca cccgcgacct cggcgcggtg cggttcttgc
     1501 ccatggtggc cggcgtcgcc ctcaatcttt aacgctctgt caaccgtgat gccgtcttaa
     1561 tgcgccgatc acaccgggtt ccgagtcagg cgatgcgcac cgagctttcc cttcccgtca
     1621 atccgtcccg aaggatcagg ctgagcggct gcgtaagcct cctggcactc ctggctgggc
     1681 tcggcggctg cagttcgatg ttcagcgtcg acgagcccgc ctggtgcgtg atgatggact
     1741 gctggttggc ggtcgagccg gtgaaggccg gctcatcgat gctgaacatg ttgctgca
//
LOCUS       DATADN010000754         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_550081, whole genome shotgun sequence.
ACCESSION   DATADN010000754 DATADN010000000
VERSION     DATADN010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550081"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..236
                     /locus_tag="VHH53_09820"
     CDS             48..236
                     /locus_tag="VHH53_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2290467.1"
                     /translation="MSTSLTRPRHGAVIAGVCAGLAERFGWSANAVRLLFVLSCLLPG
                     PQFVIYVVLWFIMPKRAY"
     gene            complement(270..1022)
                     /gene="hypB"
                     /locus_tag="VHH53_09825"
     CDS             complement(270..1022)
                     /gene="hypB"
                     /locus_tag="VHH53_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX2290468.1"
                     /translation="MYSHHEHEHGHAHEHGHEHGHEHEHGHGHGHQEPQTRTVTLEQA
                     VLARNGALAERNREWFAQHGILAVNLMSSPGAGKTTLLERTIRELGVELQVSVVEGDQ
                     ETVLDADRIRATGAPAVQINTGSGCHLDAQMLAGGLKTLDPPDSSVVLIENVGNLVCP
                     ALFDLGEQARVVITSVTEGAEKPRKYPQMFRAADLVLLNKIDLLPYVDFDVADFLEGA
                     RRANPGVEVLQLSATTGDGIDDWYGWLRARLP"
     gene            complement(1061..1393)
                     /locus_tag="VHH53_09830"
     CDS             complement(1061..1393)
                     /locus_tag="VHH53_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX2290469.1"
                     /translation="MHEMAITQSVVHAVCERMGDTPVRRVCLEIGTLSGVVADSVQFC
                     FEVITTGTPLEGASLEIVQPSGMARCRDCGAEFALDDLLVLCECGSANRELLAGEELR
                     IREVEVLA"
     gene            1465..>2330
                     /locus_tag="VHH53_09835"
     CDS             1465..>2330
                     /locus_tag="VHH53_09835"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX2290470.1"
                     /translation="MQQSIRVEVRVEGVVQGVGFRPYVHGLAGRLGLSGRVGNDTAGV
                     FVEVEGAEQTVTEFLAVLPMQAPPLAVIEQVRTSPLVPTGELGFHIVASNAAGRRDTL
                     ISADSATCADCLREMADPTDRRFDYPFINCTNCGPRFTIVRDVPYDRPFTTMAPFEMC
                     NACAAEYHNPADRRFHAQPVCCPACGPTLRLLDSDGTELPGDPLTEAAGLLTGGAVLA
                     VKGLGGYHVSTLASGNTGAAATATLRARKHREDKPFAVMVADLDAAHELCVIDEVGER
                     VLTSRRRPVVLLP"
BASE COUNT          380 a          787 c          746 g          417 t
ORIGIN      
        1 gccgccgcgg cggcagagtg gagatcggcc gccatgagga ggattcgatg agcacgtcac
       61 tgacccggcc ccgccacggc gcggtcatcg ccggggtgtg cgcgggactt gccgagcgct
      121 tcggttggag cgccaatgcg gtgcgactgc tgttcgtgct gtcttgcctg ctgcccggtc
      181 cgcagttcgt gatctacgtg gtcctctggt tcatcatgcc caagcgggcg tactgagccg
      241 atcctgaaag gtctgatcct gagaggcggt cacggcaggc gcgcgcgtag ccagccgtac
      301 cagtcgtcga taccgtcacc ggtggttgcc gacagctgca gcacctcgac acccggattc
      361 gcccggcgcg ccccttccag gaaatcggcg acatcgaaat cgacgtacgg cagcaggtcg
      421 atcttgttga gcagcaccag gtccgccgcg cggaacatct gaggatactt ccgcggtttc
      481 tccgcaccct cggtcaccga ggtgatgacc acgcgggctt gttccccgag atcgaacaag
      541 gcggggcaca ccaggttacc cacgttctcg atcagcacca ccgagctgtc cggggggtcg
      601 agcgttttca ggccaccggc cagcatctgg gcgtcgaggt gacaacccga cccggtgttg
      661 atctggaccg ccggagcgcc ggtggctcgg attcgatccg cgtcaagcac tgtctcctga
      721 tcaccctcga cgaccgacac ctgcagttcg acgcccagtt cgcgaatggt gcgctccagt
      781 agcgtggtct tacccgcccc gggtgagctc atcaggttca ccgcgagaat gccatgttgg
      841 gcaaaccatt cacggttgcg ctcggcgaga gcgccattgc gggccagcac agcctgctca
      901 agggtgacgg tacgggtttg cggttcctgg tgcccatgcc catgaccgtg ttcatgctca
      961 tgcccgtgtt catggccgtg ctcgtgggcg tgcccgtgtt cgtgctcatg gtgggagtac
     1021 acgagaatgg tgtccccaga gcagccacag gtcgcgcaca tcaggccagc acctcaactt
     1081 cccggattcg tagttcctca cccgcgagga gttcacgatt cgcgctcccg cactcacaaa
     1141 gtaccagcaa gtcgtccagc gcgaactcgg cgccgcagtc ccggcagcgg gccattccag
     1201 agggttggac aatttctaaa gaggcgcctt ccagcggagt gcccgtagtg atgacttcga
     1261 agcagaactg gaccgaatcg gccaccactc cggagagcgt gccgatctcc aggcagaccc
     1321 gccgcaccgg cgtgtcaccc atccgttcac acactgcgtg gaccacgctt tgcgtgatcg
     1381 ccatttcgtg cacccggccc tcctcggtca gcatcccagt gcagtcgcct gcggggatct
     1441 tgtcaagggc gagataatcc cctggtgcag caatctatcc gagttgaggt gcgggtcgaa
     1501 ggcgtcgtgc agggcgtggg cttccggcct tacgtgcacg gcctcgcggg ccgtctgggc
     1561 ctgtcaggcc gggtcggtaa cgacaccgcg ggcgtcttcg tggaggtgga gggagccgag
     1621 cagacggtca ccgagttcct cgcggtgctg cccatgcagg ctccgccact ggccgtcatc
     1681 gagcaggttc ggaccagccc gctggtcccg accggagagc tcggtttcca catcgtggcc
     1741 agcaacgccg ccggtcgccg ggacaccctg atctcggcgg acagcgccac ctgtgccgac
     1801 tgcctgcgcg agatggccga tcccaccgac cgccgcttcg actacccgtt catcaactgc
     1861 accaactgcg gtcccaggtt caccatcgtg cgagacgtgc cctacgaccg gccattcacc
     1921 acgatggcgc cgttcgagat gtgcaacgcc tgcgccgccg agtaccacaa tcccgctgac
     1981 cggcgctttc atgctcagcc ggtgtgctgc cccgcctgtg gcccgacgct acggctgctc
     2041 gactctgatg gcaccgagct gcccggcgac ccgctgaccg aagccgccgg gttgctcacc
     2101 ggtggcgccg tgctggccgt caaggggctc ggcggctacc acgtttcgac actggccagt
     2161 gggaataccg gcgctgccgc cactgccaca ctccgggccc gtaaacaccg cgaggacaag
     2221 ccgttcgcgg tgatggtggc tgatctggac gccgcccatg agctttgcgt gatcgacgag
     2281 gtgggggagc gggtgctgac cagccggcgg cggccagtgg tgttgctgcc
//
LOCUS       DATADN010000755         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_403776, whole genome shotgun sequence.
ACCESSION   DATADN010000755 DATADN010000000
VERSION     DATADN010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403776"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VHH53_09840"
     CDS             <1..535
                     /locus_tag="VHH53_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX2290471.1"
                     /translation="LAAHLRAVVDARCQAAGMPTPELAVEPGRAIVGPGTVTVYEVGT
                     IKDVTVGASTQRRYVSVDGGMSDNVRTALYDARYDCRLVSRRSEAPPALSRVVGKHCE
                     SGDVVVRDCWLPNDLAPGDLVAVACTGAYCYSMSSRYNLVPRPAVVAVRDGAHRLLLR
                     RETMADLLSLEMEGGVT"
     gene            532..1611
                     /gene="thrC"
                     /locus_tag="VHH53_09845"
     CDS             532..1611
                     /gene="thrC"
                     /locus_tag="VHH53_09845"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2290472.1"
                     /translation="MTLRVTADPSWPGVIEAYPDRIRVLPGCRVVTLQEGGTPLVPAP
                     TLSQRTGCEVWLKVEGANPTGSFKDRGMTLAVTEALARGAEGVLCASTGNTAASAAAY
                     AARAGIPSAVLVPRGKIALGKLAQATAYGARILAVDGNFDDCLELARKTAASYPVTAL
                     VNSVNPVRLQGQKSAAFEICDVLGRAPDVHCLPVGNAGNITAYWMGYTEYHADALVPG
                     RPRMFGFQAAGAAPLVHGAPVSHPDTIATAIRIGSPASWSGAMRARNESGGLFEAVTD
                     DQILAAYRLLAAQEGVFVEPSSATSVAGLLQVADDGRLPRESLVVCTVTGHGLKDPDT
                     ALRGIPEPEPTPVDPAAVAAALDLA"
     gene            1608..>1981
                     /locus_tag="VHH53_09850"
     CDS             1608..>1981
                     /locus_tag="VHH53_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2290473.1"
                     /translation="MSHSVLRVRVPASTANLGPGFDALGLALALYDEVEVALAPSGLR
                     VEVRGEGAGQVPTDETHLVVRALRAACARCGYRADGLVLRCHNRIPHSRGLGSSAAAA
                     VAGVLAGYALAGMQPDETVLDLA"
BASE COUNT          271 a          666 c          734 g          310 t
ORIGIN      
        1 gctggctgca catctgcggg ccgtcgtgga cgcccggtgt caagcggccg gcatgccaac
       61 cccggagctg gccgtcgagc cgggccgcgc gatcgtcggg ccgggcacgg tgaccgtcta
      121 cgaggtcggc accatcaaag atgtcaccgt gggggcatca acccaacggc gctacgtctc
      181 ggtggatggc gggatgagcg acaacgtgcg cactgcactg tacgacgcgc gttatgactg
      241 ccggctggtc tcgcgccgct ccgaggcacc gcccgcattg tcgcgggtgg tcggtaagca
      301 ctgcgagtcc ggtgacgtgg tggtccggga ctgctggttg cccaacgatc tggcgcccgg
      361 tgatctggtg gctgttgcgt gcaccggcgc gtactgctac tccatgtcca gccggtacaa
      421 cctcgtgccg cgcccagcgg tggttgcggt gcgcgacggt gcgcaccggt tactgctgcg
      481 ccgcgagacg atggcggatc tgctgtccct ggaaatggag ggcggggtca cgtgacgctg
      541 cgggtgacgg ccgacccatc ctggcctggc gtgatcgaag cctatccgga ccggatccgg
      601 gtccttccgg gctgccgggt ggtgaccttg caggagggcg gcaccccgct ggtgcctgct
      661 cccacgctgt cgcagcgcac cggctgcgag gtgtggctca aggtcgaggg cgccaacccc
      721 accggctcct tcaaggaccg ggggatgacc cttgcggtga ccgaagcgtt ggcccgcggt
      781 gcggaggggg tgctgtgcgc ctcgaccggc aacaccgctg cctctgccgc cgcctacgcg
      841 gcacgggccg ggatacccag cgccgtgctg gtaccccgcg gcaagatcgc gttgggcaag
      901 ctggcgcagg ccaccgcata cggtgcacgg atcctggccg tggacggcaa cttcgacgac
      961 tgcttggagc tggcccgcaa gacagccgcc agctacccgg tgaccgcgct ggtgaactcg
     1021 gtgaacccgg tgcggctgca ggggcagaag agcgccgcgt tcgagatctg cgacgtgctg
     1081 gggcgcgccc ctgacgtgca ttgcctgccg gtcggcaacg ccggcaacat cacggcctac
     1141 tggatgggat acaccgaata ccacgccgac gccttggtgc ccggccggcc gcggatgttc
     1201 gggttccagg cggccggtgc cgcgccactg gtgcacgggg cgccggtgtc acatccggac
     1261 accatcgcca ccgccatccg gattggtagc ccggcgtcct ggagtggcgc gatgcgggcc
     1321 cggaacgaat ccggaggatt gttcgaggcg gtcaccgacg accagatcct cgccgcctac
     1381 cggctactgg ccgcgcagga gggcgtgttc gtcgaaccgt cgtcggccac ctcggtagcg
     1441 ggactgctgc aagtcgccga cgacgggcgg ttgccccggg agtccctggt ggtgtgcacc
     1501 gtgaccgggc acgggctcaa ggatcctgac accgcgctgc gcggcattcc cgagcccgag
     1561 cccacgccgg tcgatccggc cgcggtggcc gccgcgctgg atcttgcgtg agccattcgg
     1621 tgctgcgagt acgggtaccg gcgtccaccg ccaacctcgg accgggattc gatgcgctcg
     1681 ggctggcact ggccctctat gacgaggtcg aggtggccct ggcgccgtcc ggcttgcggg
     1741 tcgaggtccg gggcgagggc gccgggcagg tgccgaccga cgagacgcac ctggtggtgc
     1801 gcgcgctgcg agccgcgtgc gcgcggtgcg gttaccgggc cgacggcttg gtactgcgct
     1861 gccacaaccg catcccgcac tcccggggac tgggttcgtc agccgcggct gccgtcgccg
     1921 gggtgctcgc cggttacgcg ctggcgggaa tgcagccgga cgagacggtg ttagacttag
     1981 c
//
LOCUS       DATADN010000756         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_235222, whole genome shotgun sequence.
ACCESSION   DATADN010000756 DATADN010000000
VERSION     DATADN010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235222"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VHH53_09855"
     CDS             complement(<1..998)
                     /locus_tag="VHH53_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2290474.1"
                     /translation="MSGPAESTSAAEDEVVGLASDLIRIDTTNTGDPDTLVGERAAAE
                     YVAGKLSEVGFETTYVESGAPGRGNVIVRLPGADPDRGALLVHGHLDVVPADASEWSV
                     HPFSGAVQDGYLWGRGAVDMKDMVAMIIAVARRFRRDGVVPPRDLVFAFLADEEAGGL
                     QGAQWLVDHRPELFEGCTEAVSEVGGFSLTVREGRRLYLVQTAEKGISWMRLRARAKA
                     GHGAVVHEDNAVTALCEAVARLGRHRFPLVMTDSVQEFLTAVGEETGLDFSGDDLEGA
                     VTKLGVLARLVGATLRDTANPTMLNAGYKANVIPSTAEAVVDCRVLPGRQAAFEREV"
     gene            1149..1233
                     /locus_tag="VHH53_09860"
     tRNA            1149..1233
                     /locus_tag="VHH53_09860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1182..1184,aa:Leu,seq:gag)
BASE COUNT          256 a          567 c          597 g          304 t
ORIGIN      
        1 acctcgcgct cgaacgccgc ctggcgcccg ggcaacaccc ggcagtccac gaccgcctcc
       61 gccgtcgagg gaatgacatt ggccttgtac ccagcgttga gcatggtcgg gttggcggtg
      121 tcgcgcagcg tcgcaccgac gagccgggcc aatacgccga gcttggtgac cgcaccttcg
      181 aggtcgtcac cggagaagtc cagcccggtc tcctcgccga cggcggtcaa gaactcttgc
      241 accgagtcgg tcatcaccag ggggaaacgg tgcctaccca accgggcgac cgcctcacac
      301 aacgcggtca ccgcattgtc ctcgtgcacc actgctccgt ggccggcctt ggcccgggcg
      361 cgcaaccgca tccaggagat gcccttctcc gcggtctgca ccaggtacag ccggcgccct
      421 tcacgcaccg tcaggctgaa cccgccgacc tcgctgaccg cttcggtgca gccctcgaac
      481 agctcgggtc ggtggtcgac cagccattgc gcgccttgca gcccgccggc ctcctcgtcg
      541 gccaggaacg cgaagaccag atcacgcgga gggacaacgc cgtcacgccg gaatcgcctg
      601 gcgacggcga tgatcatcgc gaccatgtcc ttcatgtcga ccgcgccacg gccccacagg
      661 tagccgtcct gcacggcacc ggagaacggg tgcaccgacc actcggaggc atcggcgggc
      721 accacgtcga ggtgaccgtg cacgagtagc gcgccccggt cggggtcggc accgggcagc
      781 cggacgatca cgttgccccg tccgggagcg ccggactcga cataggtggt ctcgaagccc
      841 acctcgctga gcttgccggc cacgtactcc gccgcggccc gctccccgac gagggtgtcc
      901 gggtcacccg tgttggtggt gtcgatgcgg atcaggtcgc tggccaggcc gacgacctcg
      961 tcctccgccg cgctggtgct ttctgccggt ccgctcactg ggccttccta ccacccctga
     1021 gctgggtacc gtcgcacgag tgagccagtg gcgagttggg gcgtcaggtg gcgatcggct
     1081 accctcgttg cgcgccgcac gggcagccgt gggtgcaagg tcccaatggt ggaatgtccc
     1141 atgacaatgt ccgagtggcg gaatggcaga cgcgctagct tgaggtgcta gtcccctaca
     1201 aagggggtgg gggttcaagt cccccctcgg acaccgacca taaccccaac tgtccaggtt
     1261 gaggcgggtt tcggctcgga cgagtcgttt ggcgggttcg taggtgaggt gcaggccgag
     1321 ttggtggtag acctgggcct tgtcttctgg cgcggcgtcg gcgagtactg cgtggatgtt
     1381 gccgagggcc tcgaccagcg atcggatctc gtccttgatc atccgtcgcc tgcccgtggt
     1441 ttgccgggag cgtgctagcg cctcggctcg tcgggcttgg atttcggtgg tccagcgggc
     1501 gacgagggtg gggtctgtgc cggcctcgag agcttcgcgg tagcgggtca gcttggtatc
     1561 gcactcgtgg atcacgtgtt cggccgccat tgcggttgga tcaaggccgg tatcgggctg
     1621 ggcgttgtgg agggcttcga tggtgtctgc taggcggtgt ggggtgaacg aggtgcccag
     1681 ccagtcgtct agtggtggaa ggagctcacg ttcggcgagg taga
//
LOCUS       DATADN010000757         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_697319, whole genome shotgun sequence.
ACCESSION   DATADN010000757 DATADN010000000
VERSION     DATADN010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697319"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VHH53_09865"
     CDS             complement(<1..527)
                     /locus_tag="VHH53_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2290475.1"
                     /translation="MQAVPGSDSGALVLAATPLGDCRDASARLIEVLENADVVAAEDT
                     RRLRALAAALGVTVTGRVVSHYDAVEAARIPGLLATIRAGGLVLVVTDAGMPVLSDPG
                     FRLVAACVREGLPVTCVPGPSAVTTALAVSGLPSERFCFEGFPPRRAGPRRRWLSGLA
                     GERRTCVFFESPHRLA"
     gene            588..2120
                     /locus_tag="VHH53_09870"
     CDS             588..2120
                     /locus_tag="VHH53_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX2290476.1"
                     /translation="MAGSIPPVPARPAGPLQWLAPRPLTDRWRGWIMTLGLTLVAGLV
                     RLWNLGQPTDGGTPVFDEKHYAPQAWQMLRNGGVEDNPGYELVVHPPLGKQLIALGEL
                     LMGYNGWGWRLSAAVAGTVCVLLIVRIVRRMTRSTLLGGIAGVLLIADGVSHVQSRIG
                     MLDVFLAMFVLAAFGCLVIDREQVHVRLAVVLREGRIGDTDFGPRLGVRWWRFGAGVL
                     LWLACATKWSGMYYLVAFAVMSLLWDACGRRTAGVRRPWAGTVARDLGPASWAMALVP
                     VGSYLAAWWAWLGSETGIDRHVVGNEIGTDGLFSFVPAALRALWYYSAAVLNYHEGLV
                     TPAEPHPWESKPWSWPMGLRPMLYYYESGAAAAGCGQQDCVATVMLVGTPALWWVALP
                     VLGWALWRAASGPDWRYAAALVGYAAGWLPWFLNIQRQMYFFYMTPVAPFLVIAVTLV
                     LGEVLGRARDGAERKRTGLLVVSLYIGLVVANFVWLWPILTGGSITPEHWNAQLWLPS
                     WR"
     gene            complement(2150..>2602)
                     /locus_tag="VHH53_09875"
     CDS             complement(2150..>2602)
                     /locus_tag="VHH53_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2290477.1"
                     /translation="PEMTRRAVVAGTGVLAASVALAACSSYGNPAQPAPQAPLEPVAP
                     SAPDAPPAGPAGESLASTSDIPVGGGEVFADQNVVVTQPEAGTFKAFSAVCTHQGCTV
                     SKVASGTIDCSCHGSKFAIADGSVVNGPASRPLEERQVTVSGDTIQLA"
BASE COUNT          358 a          875 c          939 g          430 t
ORIGIN      
        1 gccagccggt gcggggattc gaagaacacg caggtgcgcc gttcgccggc gaggccggac
       61 agccaccgcc ggcggggacc ggcccggcgc ggcgggaatc cctcgaaaca gaatcgttcg
      121 ctgggcaacc cggagacggc cagcgcggtg gtcaccgccg acgggcctgg cacgcaggtg
      181 accggtaagc cctcccgcac gcatgccgcc accagccgga agccgggatc tgacagcacc
      241 ggcatcccgg cgtcggtcac caccagcacg agcccgccgg cgcggatcgt cgccagcagc
      301 ccggggatgc gcgcggcctc caccgcgtcg tagtgcgaga ccacccgccc ggtgaccgtc
      361 acgcccagcg ccgcggccag tgcccgcagc cggcgggtgt cctcggcagc caccacgtcc
      421 gcgttctcga gcacctcgat caagcgtgcc gaggcgtccc ggcagtcgcc cagcggggta
      481 gcagctagta ccagtgcccc ggaatccgag cccggaacgg cttgcacgcc gccaagctta
      541 cgatcagccc cgtgacgacg gtccgggagg cgagccccga cacctcgatg gccgggtcga
      601 ttccgccggt gccggccagg cccgctggtc cgctgcagtg gctggcaccg cgcccgctca
      661 ccgaccggtg gcgaggctgg atcatgacgc tgggtttgac cctggtcgcc ggcctggtgc
      721 ggctgtggaa tctgggccaa cccaccgacg gcggcacccc ggtgttcgac gagaagcact
      781 acgcgccgca ggcctggcag atgctgcgca acggcggcgt cgaggacaac ccgggctacg
      841 agctcgtcgt gcacccgccg ctgggcaagc agctcatcgc gctaggcgag ctgctgatgg
      901 gctacaacgg ctggggatgg cggctgtccg cggcggtggc gggcacggtc tgcgtgttgc
      961 tcatcgtacg gatcgtgcgc cggatgaccc ggtcaacgct gctcggcggg atcgctgggg
     1021 tgctgctcat cgccgacggc gtcagccacg tgcagtcgcg gatcggcatg cttgacgtct
     1081 tcctggcgat gttcgtcctt gcggcgttcg gctgcctggt catcgaccgc gagcaggtgc
     1141 acgtccggct ggccgtggtg ctgcgagaag gtcgtatagg cgacaccgac ttcgggcccc
     1201 ggctgggagt gcgctggtgg cgcttcgggg ccggcgtgct gctctggctg gcctgcgcca
     1261 cgaagtggtc cggaatgtac tacctcgtgg cgttcgccgt tatgagcctg ttgtgggatg
     1321 cctgcgggcg gcgcaccgcg ggggtccgca ggccttgggc cggcaccgtg gcccgagatc
     1381 tcggaccggc atcgtgggcg atggcattgg tcccggtcgg gtcctatctt gccgcgtggt
     1441 gggcgtggct gggcagcgag accggcatcg accggcacgt ggtgggcaac gagatcggta
     1501 cggacggtct gttctccttc gtaccggcag cgctgcgggc gctgtggtac tacagcgcgg
     1561 cggtgttgaa ctaccacgag gggctggtca cccccgccga gccccatccg tgggagtcca
     1621 aaccatggag ctggccgatg ggacttcggc cgatgctcta ctactacgag tccggtgccg
     1681 ctgccgctgg atgtggacag caggactgcg tggccacggt gatgctggtc ggcacaccgg
     1741 cgctgtggtg ggtcgcgctg ccggtgctgg gctgggcgtt gtggcgggcg gccagcggac
     1801 cggactggcg ctacgcagca gccctggttg gctacgccgc cggctggctg ccctggtttc
     1861 tcaacatcca gcggcagatg tacttcttct acatgacgcc ggtggcaccg ttcctggtca
     1921 tcgcggtgac cttggtgctg ggcgaggtac tcggtcgggc tcgagatggc gccgagcgta
     1981 agcgcaccgg cctgctggtg gtctcgctgt acatcggtct cgtggtggcc aacttcgtgt
     2041 ggctctggcc gatcctcacc ggtggctcga tcaccccgga gcactggaac gcccagctgt
     2101 ggttgccttc ctggcgctga gtcgtcctcg gttgcacggt cgcggctgat caagccagct
     2161 ggatcgtgtc acccgagacc gtgacttggc gttcttccag cgggcgtgac gcgggaccgt
     2221 tgacgaccga accgtccgcg atggcgaatt tgctcccatg gcaggaacag tcgatcgtcc
     2281 cggatgccac cttgctcacc gtgcagccct ggtgagtgca gaccgccgag aacgccttga
     2341 aggttcccgc ctccggctgg gttaccacca cattttgatc ggcgaagacc tcgccgccgc
     2401 ccactgggat gtcggaggtg ctcgccagcg actcgcctgc cgggcctgcc ggcggggcgt
     2461 ccggcgcaga aggagctacc ggttccagtg gggcttgcgg tgccggctga gccggattcc
     2521 cgtaactgct gcatgcggcg agcgctacgg aagcagccag cacccccgtg cccgccacga
     2581 cggcgcgccg ggtcatctca gg
//
LOCUS       DATADN010000758         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_404258, whole genome shotgun sequence.
ACCESSION   DATADN010000758 DATADN010000000
VERSION     DATADN010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404258"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /gene="ppk1"
                     /locus_tag="VHH53_09880"
     CDS             complement(<1..1630)
                     /gene="ppk1"
                     /locus_tag="VHH53_09880"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX2290478.1"
                     /translation="MTPVLPALPAATPLPLATDLPDDRYLNRELSWLDFNARVLALAE
                     DSSQPLLERAKFLAIFASNTDEFYMVRVAGLKRREEAGLSVRSADGLTPREQLARIAA
                     RSRELAELHARVFIDEVCPQLAEHGITFVTWSDLDDAQQLRLSGYFTDQIFPVLTPLA
                     VDHAHPFPYISGLSLNLAVMVRDPDSRIERFARVKVPNNVPRLVQVGTNQGERGSPRA
                     TFLPLEELIGAHLNELFAGMDVVDWHIFRITRNADFEVEEDRDEDLLKALERELARRR
                     FGPPVRLEVADTMSEHVLKVLLRELDVDPHDVVEVPGLLDLSCMWQVYSLPAPELKDE
                     PFIPVTHPAFGEGESPKDVFSTLGDGDVLVHHPYDSFSTSVQRFIEQAAVDPHVLAIK
                     QTLYRTSGDSPIVDALIDAAEAGKQVVALVELKARFDEQANIRWARALERAGVHVAYG
                     LVGLKTHCKTALVVRQEGSMIRRYCHIGTGNYHPKTAGLYEDVGLFTAEPTIGADVTD
                     LFNSLTGYARQTRYRSLLVAPYGIRRGIVERIEGE"
     gene            complement(1976..2632)
                     /gene="cofC"
                     /locus_tag="VHH53_09885"
     CDS             complement(1976..2632)
                     /gene="cofC"
                     /locus_tag="VHH53_09885"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX2290479.1"
                     /translation="MQVDLLLPVKPLVQAKTRLRGVVEAGAGSGVGASEAHARLVVAL
                     VRDTLAAVAGAAVVRRAVAICSDEVVRTVLAADGVQTIPDEPAAGLNPALRYGEAVLR
                     GADPTATVGALQADLPALQPEELDCAVRTGLITGGRAFCTDRTGTGTTLLLASPGQSL
                     NPCFGPGSAAAHRATGARELTGCWPGLRCDVDTAADLTIAYALGLGRFTRATLGGTQA
                     "
     gene            2725..3498
                     /locus_tag="VHH53_09890"
                     /pseudo
     CDS             2725..3498
                     /locus_tag="VHH53_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674004.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
     gene            complement(3486..3642)
                     /locus_tag="VHH53_09895"
                     /pseudo
     CDS             complement(3486..3642)
                     /locus_tag="VHH53_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
BASE COUNT          616 a         1186 c         1239 g          601 t
ORIGIN      
        1 tctcaccctc gatccgctcc acgatgccgc gccggatgcc gtagggcgcc accagcagac
       61 tgcggtagcg agtctgccgc gcgtagccgg tcagtgagtt gaacaggtcg gtgacgtcgg
      121 caccgatcgt cggttcggcg gtgaacagtc cgacgtcctc gtagaggccg gccgtcttcg
      181 ggtggtaatt gccggtgccg atatggcagt aacggcggat catggagcct tcctgccgca
      241 ccaccagggc agtcttgcag tgcgtcttga gccccaccaa gccgtaagcc acgtgcaccc
      301 cggcccgttc cagcgccctg gcccacctga tattggcctg ctcgtcgaac cgtgctttga
      361 gttccaccag tgcaacgacc tgcttgccgg cctcggcagc gtcgatgaga gcgtcgacga
      421 tcggcgagtc ccctgacgtt cggtagagcg tctgtttgat cgccagtacg tggggatcca
      481 ccgcggcctg ctcgatgaaa cgttgcacac tcgtggaaaa cgagtcatag ggatggtgca
      541 ccagcacatc accgtcaccc agggtggaga acacatcctt gggtgattcg ccctcaccga
      601 aggccgggtg cgttacgggg atgaacggct cgtccttcag ctccggggcg ggcagcgagt
      661 agacctgcca catgcacgac aggtcgagca ggcccggcac ttcgacgacg tcgtgcgggt
      721 cgacgtcgag ctcgcgcagc aggaccttca ggacgtgctc gctcatcgtg tcggccacct
      781 cgaggcgcac cggtggaccg aagcggcgtc gcgcgagctc ccgttccagc gccttcagca
      841 ggtcctcgtc acggtcctcc tcgacctcga aatcggcgtt gcgggtgatc cggaagatgt
      901 gccagtccac cacgtccatg ccggcgaaaa gctcgttgag atgggccccg atcagctctt
      961 ccagcggtag aaaggtggcg cgtggtgacc cgcgctcacc ctggttggtg cccacctgga
     1021 ccaggcgtgg cacattgttg ggcactttga ctcgggcgaa tcgttcaatg cgactgtcgg
     1081 gatcacgaac catcacggcc aggttcagcg acaagccgga gatgtaagga aatgggtgtg
     1141 cgtggtccac cgccaatggg gtgagcaccg gaaagatctg atctgtgaag tagcccgaca
     1201 gccgtaactg ctgcgcgtcg tccaagtcgg accacgtgac gaaggtaatg ccgtgttcgg
     1261 caagctgcgg gcacacctcg tcgatgaata ctcgggcgtg cagctcggcg agttcgcggc
     1321 tgcgcgccgc gatccgggcc agctgctcgc ggggggtcaa cccgtccgca ctgcgcactg
     1381 acaggccggc ctcttcacgg cgcttgagcc cggcgacccg gaccatgtag aactcgtcgg
     1441 tgttcgacgc aaagatcgcc aggaactttg cccgctccag cagcggctgg ctggagtcct
     1501 cggccagcgc gagcacccga gcgttgaaat ccagccagga cagttcccgg ttgagatagc
     1561 ggtcgtcggg gaggtcggtg gccaagggca gcggagtcgc cgctggtagg gcgggtagca
     1621 ccggtgttat ggcgccgtcg ctggccggtg cggcgcggcc ggtcaacgcg gtagcgccgg
     1681 agcgggcacc gcggccagcc ggttggagat catggtgagc agcggcgttc cgtggcgtgc
     1741 tactgggcga cgagctgttg gctgcggtcg tgacaccggt gtcttcgtcg tgggtgctca
     1801 ccccgccatt gttccgtaca aagcattagc ccggcggatg tacacatact tcagtggtac
     1861 cgacggtcgg atttcgaatt atcatcacgc atggtgagca gccgcctact cgctgcataa
     1921 gccggttttc caggtctcgt gcaatggcgg aatagcgaat ccggactacg cggaatcacg
     1981 cctgcgtgcc gcccagcgtg gcgcgggtga accggccaag acccagagcg tatgcgatgg
     2041 tcagatcggc tgcggtgtcg acgtcacacc gcagccctgg ccagcagccg gtgagctcgc
     2101 gagccccggt ggcccgatgc gcggccgccg agccaggtcc aaagcaggga ttcagagact
     2161 gtcctggcga ggcgagcagc agggtcgtcc cggtgccggt ccgatcggtg cagaaggccc
     2221 ggccgccggt gatcaggccg gtgcgaaccg cgcagtccaa ctcctccggc tgcagcgccg
     2281 gcagatcggc ctgcagggca ccgaccgtcg cagtagggtc cgctccgcgc agcactgcct
     2341 ctccgtatcg cagggcgggg ttcaggccgg cggccggctc gtcgggaatt gtctgcaccc
     2401 cgtccgccgc gagaacagtg cgcactacct cgtcggagca gatcgccact gcccggcgca
     2461 ccaccgccgc gccggccact gccgcgaggg tgtcccggac cagtgctacg accaaccggg
     2521 cgtgcgcctc ggaagcaccg accccgctgc cggctccggc ctcgacaacg ccgcgcagcc
     2581 gcgtcttggc ttggacgagc ggtttcactg gaaggagcag gtccacctgc acgtgcccat
     2641 cctcccgcgc tggacctccg atccagtggc aggaagactc aggtcgatta gcgcaaggag
     2701 gatggcgtgg cacgcgacgg cgtcatgacc ctgccggtgc agcgaatcgc ggtgatgggc
     2761 gcgggctcat ggggtaccac cttcgccaag gtgctcgccg acgccggccg ggatgtcgtg
     2821 ctctgggccc gtcgggctga ggtggccgac gcggtcgccc accggcagac caacccggac
     2881 tacctgcccg gagtccgatt gccgtcgtcc ttgcaggcca ccgaacacgc cgacgaggct
     2941 ttggaccagg cagacgcggt ggtattcgcg gtaccgagcc agacgctacg ggacaacctg
     3001 ggcggttggc acaagttcat tcgggctgat gccaccttgg tgagcctggc caagggcgtc
     3061 gagctcggca gcctcaagcg gatgagcgag gtcgtcatag aggtgacagc cgtcacgcca
     3121 aatcaggtgg tggtggtctc gggccccaat ctggctcggg agatcgccgc cgagcagccc
     3181 accgcgagcg tggtcgcctg catcgaccac gaccgtgccg ttgcggtgca gcacgcctgc
     3241 gtgaccggat atttccggcc ttacaccaac accgacgtca tcggctgcga gcttggcggc
     3301 gcatgcaaga acgtcatcgc actggcctgt gggatggcgg ttggattggg atacggggac
     3361 aacaccctcg caacgttgat cacgcgaggg ctggctgaga tggggcgcct gggtgcggcg
     3421 ctgggcgccg acccgatgac cttcgcaggc ctggccggcc ttggcgatct ggtggccacc
     3481 tgcggctcat cgctgtctcc caacccgagc cggtagccga aagagcggtt gcgagacagc
     3541 gatgagccgc aggtggccac tagatcgcca agaccggcca ggcctgcgaa ggtcatcggg
     3601 tcggcgccca gcgccgcacc caggcgcccc atctcagcca gc
//
LOCUS       DATADN010000759         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_837334, whole genome shotgun sequence.
ACCESSION   DATADN010000759 DATADN010000000
VERSION     DATADN010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837334"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1818)
                     /locus_tag="VHH53_09900"
     CDS             complement(<1..>1818)
                     /locus_tag="VHH53_09900"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2290480.1"
                     /translation="DYVGRDGVADMGELAGRLATASTLPIMLDSTEPEVIRAGLERLG
                     GRCAVNSVNYEDGSGPESRFARTMELVREHGAAVVALCIDEEGQARTAEWKVRVAKRL
                     IEDLTSNWGMRLGDIIVDTLTFPISTGQEEVRRDAIETIEAIRELKRLYPEVRTTLGL
                     SNVSFGLNPAARQVLNSVFLHECVQAGLDTAIAHSSKILPMARIPDEQREVALDLVYD
                     RRRDGYDPLQRLMELFEGATVSSSKASRAEELAALPLFERLQRRIVDGERNGLESDLD
                     DALVEKSALQIINDTLLAGMKTVGELFGSGQMQLPFVLQSAEVMKAAVAYLEPHIDKS
                     DDAGKGRIVLATVKGDVHDIGKNLVDIILSNNGYEVVNIGIKQPVNAILEAAEEHRAD
                     VIGMSGLLVKSTVIMKENLEEMNSRGVAARWPVLLGGAALTRAYVENDLSEVYVGDVH
                     YARDAFEGLRLMDAIMAAKRGQTPLVDAELKAKAEERRARRQRSLRIAEARRAADAPR
                     EVEGPARSDVATDVPVPAPPFWGTRVHRGIHAVAYTRLLDERATFMGQWGLRGSRGGK
                     GPSYEELVETEGRPRLRQWLARIHEEGIVNAAVVYGYFP"
BASE COUNT          306 a          713 c          513 g          286 t
ORIGIN      
        1 cacgggaagt agccgtagac gacggcggcg ttgacgatgc cctcctcgtg gatgcgcgcg
       61 agccactgcc gcagccgcgg cctgccctcg gtctccacga gctcctcgta cgagggcccc
      121 ttgccgccgc gcgagccgcg caggccccac tggcccatga acgtcgcccg ctcgtcgagc
      181 aacctggtgt aggccacggc atggatcccg cggtgcaccc gggtgcccca gaacggcggc
      241 gccgggaccg gaacgtcggt ggccacgtcg gaccgggccg ggccctcgac ctcccggggt
      301 gcgtcggcgg cgcggcgggc ctcggcgatg cgcagcgacc gttgccggcg ggcgcggcgc
      361 tcctcggcct tggccttgag ctcggcgtcg accagcgggg tctggccgcg cttggccgcc
      421 atgatggcgt ccatcagccg caacccctcg aaggcatccc gcgcatagtg cacgtcgccg
      481 acatacacct ccgacagatc gttttccaca taagcacggg tcaacgccgc cccacccagc
      541 agcaccggcc accgcgcggc caccccccgg gagttcatct cctcgaggtt ctccttcatg
      601 atcaccgttg acttcaccag caacccggac atcccgatca catccgcccg gtgctcctca
      661 gccgcctcca aaatcgcgtt caccggctgc ttgatcccga tattgacaac ctcataacca
      721 ttgttggaca agatgatgtc caccaggttc ttgccgatgt catgcacatc gcccttgacc
      781 gtggccaaca cgatccggcc cttacccgca tcatcggact tgtcgatatg cggctccaga
      841 tacgccaccg ccgccttcat cacctcagcc gactgcaaca cgaacggcaa ctgcatctgc
      901 cccgacccga acagctcacc caccgtcttc atcccggcca gcaacgtgtc attgatgatc
      961 tgcagcgccg acttctccac cagcgcatca tccagatcgg actccagacc gttgcgctca
     1021 ccgtcgacga tgcgccgctg caaccgctcg aacagcggca gcgccgccaa ctcctccgcc
     1081 cgcgacgcct tcgacgacga cacggtcgcg ccctcgaaca gctccatcaa ccgctgcaac
     1141 gggtcgtagc cgtcgcgccg ccggtcatag accagatcca gcgccacctc gcgctgctcg
     1201 tccgggatcc gggccatcgg caggatcttc gacgagtgcg cgatcgccgt gtccaacccg
     1261 gcctgcacgc actcgtgcag gaacaccgaa ttgagcacct gccgcgccgc cgggttcagc
     1321 ccgaacgaga cgttggacag cccgagcgtg gtgcgcacct ccgggtacag ccgcttgagc
     1381 tcgcggatcg cctcgatggt ctcgatggcg tcgcggcgca cctcctcctg ccccgtggag
     1441 atcggaaacg tcagcgtgtc gacgatgatg tcgcccagcc gcattcccca gttcgaggtc
     1501 aggtcctcga tcagccgctt ggccacccgc accttccact cggcggtgcg ggcctgcccc
     1561 tcctcgtcga tgcacagcgc gaccaccgcg gcgccgtgct cccgaaccag ctccatggtg
     1621 cgtgcgaacc gcgactccgg accggaaccg tcctcatagt tcaccgagtt gaccgcacac
     1681 cggccaccca gccgctccag accggcccgg atcacctccg gttcagtcga gtcaagcatg
     1741 atcggcagcg ttgaagcggt ggccaaccgt ccagccagct cccccatatc ggccacgcca
     1801 tcccggccga catagtcc
//
LOCUS       DATADN010000760         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_837341, whole genome shotgun sequence.
ACCESSION   DATADN010000760 DATADN010000000
VERSION     DATADN010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837341"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VHH53_09905"
     CDS             <1..401
                     /locus_tag="VHH53_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX2290481.1"
                     /translation="ALVAATSERGYLAVQAYLDRVADASAAVLRTELARRTHLQTTFG
                     WGPRFLHSTGQYHKGGYPNGAFLQLTGEVVDDIPVPGTDFTLGELQRAQAVGDAEVLA
                     AHGRPVLRLHLADRVAGLIQVVRAVHELRL"
     gene            398..>2150
                     /gene="zwf"
                     /locus_tag="VHH53_09910"
     CDS             398..>2150
                     /gene="zwf"
                     /locus_tag="VHH53_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX2290482.1"
                     /translation="MTSTENWINPLLDARDKRLPRIAGPCGVVIFGVTGDLSRKKLMP
                     AIYDLANRGLLPPGFALVGFARRDWADQNFAELVHESVREHARAPFRQEVWDRLVEGV
                     RFVAGSFDDDAAFDDLASVVENLDRERGTGGNHAFYLSIPPAMFPVVLKQLARTGLAA
                     RNGDQWRRVVIEKPFGHDLRSARELNEIVNDVFPEESVFRIDHYLGKETVQNILALRY
                     ANQLYEPIWNSHYVDHVQITMAEDIGLAGRAGYYDGIGAARDVIQNHLLQLLALTAME
                     EPLSFSPGSLRGEKIKVLSATKLVEPLAETTARGRYIAGWQGGTKVVGVLEEAGFHRD
                     STTETFAAITLHVESRRWAGVPFFLRTGKRLGRRVTEIAVMFKRAPHLPFEETMTEEL
                     GQNALVVRVQPDEGVTIRFGSKVPGTAMEVRDVTMDFRYGTAFTESSPEAYERLLLDV
                     LLGEPSLFPVNAEVELSWAILDPVLEHWAKQDAPETYAAITLGIDTRRWAGVPFYMRT
                     GKRLGRRVTEIAVVFKRAPHLPFDDTATEELGQNALVIRVQPDEGVTLRFGSKVPGAT
                     MEVRDVSMDFSYGHSFTE"
BASE COUNT          359 a          707 c          723 g          361 t
ORIGIN      
        1 cggctctggt cgcggccacc tctgagcgtg gctatctcgc ggtgcaggcg tacctggacc
       61 gggtagccga tgcctccgca gcagtgctgc gaaccgaact ggctcgccgc acccacctgc
      121 agaccacctt cggctggggg ccgcggttcc tgcactcgac tggccagtac cacaagggcg
      181 gctacccgaa cggggcgttc ctgcagctaa ccggtgaggt cgttgacgac atccccgtgc
      241 ccggcaccga tttcaccctc ggagagttgc agcgggccca ggctgtgggc gacgccgaag
      301 tgctcgccgc ccatggccgt ccggtgctgc ggctgcatct ggccgatcgg gtggcaggac
      361 tgatccaggt ggtgcgcgcg gtgcacgagc tacggctatg accagcactg agaactggat
      421 caatccgctg ctggatgctc gagacaagcg actgccccgg attgccggtc cgtgcggtgt
      481 tgtgatcttc ggggtcaccg gcgatctgtc ccgcaaaaag ctgatgccgg cgatctatga
      541 cctggccaac cgcgggctgc tgccgcctgg cttcgccttg gtcgggttcg cccgccggga
      601 ttgggccgac caaaacttcg ccgaactggt gcacgagtca gtccgggaac atgcccgggc
      661 tccttttcgc caggaggtgt gggaccggct ggttgaaggc gtcaggttcg tggctggcag
      721 ctttgatgac gacgccgcgt tcgacgacct cgcgtcggtg gtcgaaaatc tcgatcggga
      781 acggggcacc ggcggcaacc acgccttcta cctgtccatc ccgcccgcaa tgttcccggt
      841 ggtgctcaaa cagctggccc gcacaggtct ggcggcacgc aacggcgacc aatggcgccg
      901 ggtggtcatc gaaaagccgt tcgggcacga cctgcgcagc gcccgcgaac tcaacgagat
      961 tgtcaacgac gtcttccccg aggaatcggt attccgcatt gatcactacc tcggcaagga
     1021 gaccgtccag aacattctcg cgttgcgcta cgccaaccag ctctacgagc cgatctggaa
     1081 cagccactac gtcgaccacg tgcagatcac tatggccgag gacatcgggc tggccggccg
     1141 cgccggctac tacgacggca tcggggcggc tcgcgacgtg atccagaacc acctgttgca
     1201 gctgctcgct ctcaccgcga tggaggagcc gctgtccttc tctcccggtt cgctgcgcgg
     1261 cgagaaaatc aaagtgctct ccgcgaccaa actcgtggag ccgttggctg agaccaccgc
     1321 gcgcggccgg tacatcgccg gatggcaggg cggcaccaag gttgtcgggg tgctcgaaga
     1381 agcgggattc caccgagact ccaccaccga gaccttcgcc gcgatcacgc tgcacgtgga
     1441 gagccggcga tgggccgggg tgccgttctt cctgcgcacc ggcaagcggc tgggccggag
     1501 ggtcaccgag atcgcggtga tgttcaagcg ggctccgcac ctgcccttcg aggagaccat
     1561 gaccgaggag ctggggcaga acgccctggt ggtccgggtt caaccggacg agggtgtcac
     1621 gatccggttc ggctccaagg tgcccggaac cgcgatggag gtccgtgacg tcacgatgga
     1681 cttccgctac ggcacggcgt tcaccgaatc ctcccccgag gcctacgagc ggttgctgct
     1741 ggatgtactg ctcggcgagc cgtcgttgtt cccggtcaac gccgaggtgg agctgtcctg
     1801 ggcaatcctg gatccggtgc tggaacactg ggcgaagcag gacgcgcccg agacctacgc
     1861 cgcgatcacc ctcgggatcg acacccggcg ctgggccggc gtcccgttct acatgcgcac
     1921 gggcaagcgg ctgggccggc gggtcaccga gatcgcggtg gtgttcaagc gcgcaccgca
     1981 cctgccgttc gacgacaccg cgaccgagga gctcggccag aacgcgctgg tgatccgggt
     2041 gcagccggac gagggcgtga cgctgcggtt cggctcgaag gtccccggcg ccaccatgga
     2101 ggtgcgggac gtctcgatgg acttcagcta cgggcactcg ttcaccgagt
//
LOCUS       DATADN010000761         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_837437, whole genome shotgun sequence.
ACCESSION   DATADN010000761 DATADN010000000
VERSION     DATADN010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837437"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1556)
                     /locus_tag="VHH53_09915"
     CDS             complement(<1..>1556)
                     /locus_tag="VHH53_09915"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2290483.1"
                     /translation="MLELGQPLHAFDAAKLDGDLVVRRATEGEKLTTLDGVCRELDPD
                     DMVICDSSGPVSLAAVMGGESTEVGVQTTSLLLEAAIWDPASVARSARRHKLPSEAAR
                     RFERGVDPALPPVAAELAATLLSRYGGGEIEQGRTDVGTPPRPRHVTMPIDLPDRVAG
                     RRYENGATVRRLSQIGCAVTLGGSNGTTTVTVVPPSWRPDLSQPADLVEEVLRLEGYD
                     TIPSELPPAPPGRGLSAEQRRRRTISRALAAAGYVEVLPSPFVSAGIWDAFGLPDDDP
                     RRHTVTLLNPLETDRDQLSTTLLPGLLEALVRNVARGQRDVLLYGMAPVVQPRVATTP
                     MPHVRVDRRPSHDQLAALNRALPVQPLHVGVVLAGQYERAGWWNTVRAAIWADAVQAA
                     RLVGHAAGVEIAVRAAQTAPWHPGRCAQLVVDGRVLGHAGELHPAVIEALGLPTRTCA
                     MELDLDALPLTERRPAPVVSPYPPVLQDVALVVDAAVPAAELTATLRRGAGELLEDIR
                     LFDVYTSDQI"
BASE COUNT          249 a          565 c          529 g          213 t
ORIGIN      
        1 gatctgatca ctggtgtaga cgtcgaacag gcgaatgtcc tccagcagtt caccggcgcc
       61 acggcgcagg gtggccgtca gctcggcggc aggcacggcc gcatccacca ccaatgcgac
      121 gtcctgcaat accggcgggt agggcgacac caccggtgcg ggccgccgct cggtcagcgg
      181 cagcgcatcg aggtcgagct ccatcgcaca ggtccgcgtc ggtaacccga gcgcctcgat
      241 caccgcgggg tgcaactccc cggcgtggcc cagcacgcga ccatccacca ccagctgcgc
      301 gcaccggccg ggatgccacg gcgcggtctg agcagcacgc accgcaatct ccactcccgc
      361 ggcgtggcca accagtcgcg cggcctgtac agcatcggcc cagatcgccg cccggacggt
      421 gttccaccag ccggcacgct cgtactgccc ggcgagcacg acacccacgt gcagcggctg
      481 tacgggcaac gcacggttca gcgcggcgag ctggtcgtgg cttggtcgtc ggtccacccg
      541 cacgtgcggc atcggcgtgg tagcaacccg tggctgcacg accggggcca tgccgtagag
      601 cagcacatcg cgttggccgc gtgccacgtt gcgcaccaag gcttcgagca ggccgggcag
      661 cagcgtggtg gacaactggt cgcggtcggt ctccagcggg ttgagcagcg tcacggtgtg
      721 ccgtcgggga tcgtcgtcgg gcaagccgaa tgcgtcccag atcccagctg agacgaacgg
      781 gctgggcagc acctccacgt acccggcggc ggccaacgcc cgagagatcg tccggcggcg
      841 gcgctgctcg gcggacaggc cgcgaccggg tggcgccggc gggagctcag aggggatggt
      901 gtcgtagccc tccagccgca gcacctcctc gacgaggtcg gcgggttggg acagatcggg
      961 ccgccaggac ggcgggacga cggtgaccgt cgtggtgcca ttgctcccgc cgagcgttac
     1021 ggcacagccg atctgggaca gccggcggac cgtggcaccg ttctcatagc gccgccccgc
     1081 cacccgatcc ggcagatcga tcggcatcgt cacatgcctg ggccgcgggg gggttccaac
     1141 atcggtacgg ccttgctcga tctcgccgcc gccatagcga cttagcagcg tcgcagcgag
     1201 ctcggcagcc accggaggca gggcaggatc caccccacgt tcgaaacgcc gggcggcctc
     1261 gctgggtagt ttgtgccgcc gggcgctgcg cgccaccgag gccggatccc agatcgccgc
     1321 ctccagcagc aggctggtgg tctgcacacc gacctcggtg ctttccccac ccatcaccgc
     1381 cgccagcgac accgggccgc tgctatcgca gatcaccatg tcgtcgggat ccagctcccg
     1441 gcataccccg tccaaggtgg tgagcttctc gccctcggta gcacgccgca cgacgaggtc
     1501 accgtccaac ttggcggcgt caaaggcatg cagcggctgc ccgagctcca gcatca
//
LOCUS       DATADN010000762         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_235816, whole genome shotgun sequence.
ACCESSION   DATADN010000762 DATADN010000000
VERSION     DATADN010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235816"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..805)
                     /locus_tag="VHH53_09920"
     CDS             complement(53..805)
                     /locus_tag="VHH53_09920"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2290484.1"
                     /translation="MLVPLYEAVYERLDVGPATRLLGLGCGSGLALLMAAARGAAVTG
                     VDASPERMALARQRLLPEAGATRARAGTRLVDGSPRDTKEPGAPAYTLVTAFEPIGCL
                     AGDSEGLGGLLAEATPLAERRAPVVLAGWGPPERCATTAVLRVATKLAEPLRSAGSWR
                     PALRDDLEDVAQRAGLEPDGSGRVACPFGYADVDSAVRGLKSTGLFDAAITATDQVQV
                     DKELAEALHPHQRQDGTVWMPNVFRYLIARTR"
     gene            1107..>1582
                     /locus_tag="VHH53_09925"
     CDS             1107..>1582
                     /locus_tag="VHH53_09925"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2290485.1"
                     /translation="MAKAPVLTPQADDFPRWYQDLITKAELADNGPVRGTMVIRPYGY
                     GLWERMQAEMDARIKETGTQNAYFPLLIPQSYLTREAEHVEGFAPELAVVTHGGGKEL
                     EEPAVVRPTSEMIINDYFSKWVQSYRDLPLLINQWCNVMRWEMRTRLFLRTAEFLW"
BASE COUNT          252 a          594 c          504 g          232 t
ORIGIN      
        1 tcacaccatt cacacccgca ctgaaatcac acccgcactg aaaaactgtt cgtcaccgtg
       61 tccgggcgat caggtaccgg aacacgttcg gcatccacac cgtcccgtcc tgccgctggt
      121 gcggatgcag ggcctcggcc agctccttgt ccacctgcac ctggtccgtc gccgtgatcg
      181 ccgcgtcgaa caaccccgtc gacttgagcc cgcgcaccgc gctgtccaca tcggcgtacc
      241 cgaaggggca cgccacccgc ccggacccgt ccggctcgag accggcccgc tgggcgacgt
      301 cctccaggtc gtcgcggagg gcgggacgcc agctgcccgc gctgcgcagc ggctccgcca
      361 gcttggtggc gacccgcagc acggccgtcg tggcgcaccg ttccggcgga ccccagccgg
      421 ccagtaccac gggcgcccgg cgctcggcga gcggcgtcgc ctccgcgagc agcccgccca
      481 gcccctccga gtcgcccgcg aggcacccga tcggctcgaa ggcggtcacc agggtgtacg
      541 cgggagcgcc cggctccttg gtgtcccggg gcgagccgtc gaccagccgg gtgcccgccc
      601 gcgcgcgcgt ggctcccgcc tcgggcagca accgctgccg tgcgagggcc attctttcgg
      661 gggaagcgtc gacaccggtg accgccgctc cccgggcggc ggccatcagc agggcgagtc
      721 cggaaccgca gccgaggccg agcagccgcg tcgcgggacc cacgtccagt cgctcgtaga
      781 cggcctcgta gagcggtacg agcatccgct cctggatttc ggaccagtca cgcgcgcgtg
      841 cacgcaggtc cacgcggggt gcggcccccg cgtgaggcag gtgctgccgc acgagcgtag
      901 gtgtcataag taagcgcccc aatccgccga gagttgcctc ttgcccgatt ccatggcccc
      961 cgtgacagtg cgctcgcact acccccgtat gccaggaaac tcccccctcg acctgtcgtc
     1021 cagtggaatg cggcccggta tatgagttgt catgtgccta tggcgagatt tcacattccg
     1081 gcaacgtggg cccgtaccat ctcgccatgg ccaaggcacc cgtactcacc ccccaggcgg
     1141 acgatttccc gcgctggtac caggacttga tcaccaaggc ggagctcgcc gacaacggac
     1201 cggtgcgcgg caccatggtc atccgaccgt acgggtacgg gttgtgggag cggatgcagg
     1261 ccgagatgga cgcccgcatc aaggagaccg gcactcagaa cgcgtacttc ccgctcctga
     1321 tcccgcagtc gtacctcacc cgcgaggcgg agcacgtcga gggcttcgcg cccgagctgg
     1381 ccgtggtcac gcacggcggc ggcaaggaac tcgaagagcc cgccgtggtc cggcccacct
     1441 ccgagatgat catcaacgac tacttctcga agtgggtcca gagctaccgc gacctgccgc
     1501 tgctcatcaa ccagtggtgc aacgtcatgc gctgggagat gcgcacccgg ttgttcctcc
     1561 gcaccgccga attcctctgg ca
//
LOCUS       DATADN010000763         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1288742, whole genome shotgun sequence.
ACCESSION   DATADN010000763 DATADN010000000
VERSION     DATADN010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288742"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..876
                     /locus_tag="VHH53_09930"
     CDS             58..876
                     /locus_tag="VHH53_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2290486.1"
                     /translation="MPEGHTTHRLARRHQQCYGGSPVAVSSPQGRFAAAAAKLDGRVL
                     QRAEAHGKHLFHVYSPDLVVHVHLGLYGSFSDATLPVQPPQGQVRMRLVGSTHWTDLR
                     GPAACDLLTGTEVDAVRARLGPDPLRRDADPDQAWARISRSRAPLATLLMDQVVVSGI
                     GNVYRAELLFRHRLDPLQPGCVLREDQWSALWSDLVELMHDGVRRGRIDTVRPEHDPV
                     AMGRPPRQDRHGGEVYVYRRAGAPCLVCATDVVTARHAGRNLYWCPRCQRRPVV"
     gene            complement(1141..1530)
                     /locus_tag="VHH53_09935"
     CDS             complement(1141..1530)
                     /locus_tag="VHH53_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HEX2290487.1"
                     /translation="MAQLRTLGRAGPLSCDVLDLQVTEHGPQVRVVTVGGEIDALTAP
                     ELDVLLTAQLAAASLVVVDLERVRSLGSAGLSVLFAAAELATRENRYLWLVVPRRGMA
                     NRALEATGLGERFNVAETVAAVLINSL"
     gene            complement(1894..2388)
                     /locus_tag="VHH53_09940"
     CDS             complement(1894..2388)
                     /locus_tag="VHH53_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290488.1"
                     /translation="MRPTAVTDNGEVPDALIAEHGFSALVAVAKGDREYRLLYDAGTS
                     PDGVVENMRRRGHAGVVNIARYARRLTGDQPLYALLGGFHLNGPVFEPLIPPVLDDLH
                     PSVLVPAHCTGWRAQHAMSTRFGPAFIPTASAPASTCNNKGRPVADARPLLLDMRARL
                     RVHA"
BASE COUNT          479 a          899 c          921 g          471 t
ORIGIN      
        1 ctcggcgttc gagtccaccg gggagccacc cccgatgtcc acgcactgag tctggcgatg
       61 ccggaggggc atacgacgca ccggttggct cggcggcacc agcagtgtta cggcgggtca
      121 ccggtggcgg tgtccagccc gcagggtcgc ttcgcggcgg ccgctgcaaa gcttgacggt
      181 cgggtgttgc agcgtgccga agcgcacggc aagcacctgt tccacgtgta cagccctgac
      241 ctggtggtgc acgtacacct tggtctttac ggttccttca gcgacgcgac attgcccgtg
      301 cagccgccgc aggggcaggt gcgcatgcgg ctggtcggct ccacgcactg gacagatctg
      361 cgggggccgg cggcctgtga cctgctcacg ggaaccgagg tggacgcggt gcgcgcccgg
      421 ctggggccag atccgctgcg ccgcgacgct gacccggacc aggcctgggc ccgcatctcc
      481 cggtcccggg cgccgctggc cacgttgttg atggatcagg tggtggtctc gggaatcggc
      541 aatgtctatc gggctgagtt gttgttccgc caccggctgg atccactgca gccagggtgt
      601 gtgcttcgcg aggatcagtg gtcggcactg tggtcagatc tcgtcgagtt gatgcacgac
      661 ggggtgcgtc gtggccggat cgatacggtc cgtccggagc atgacccggt tgcgatggga
      721 cgacctccgc gtcaggatcg gcatggcggc gaggtctatg tctaccgccg ggccggtgcg
      781 ccgtgcttgg tgtgcgctac cgacgtcgtc actgcgcggc acgccgggcg caacctctat
      841 tggtgtcctc gctgccagcg gcggccggtg gtttagaact cggtggccct caatatcacc
      901 gccaccccag tcccaccgct ggatcagccg tcggtgggcc acgtcgtagc tggccagctt
      961 gtcggtgacc agcacccgcg gcacgaaccg taaccccctc aggagctggc gaaagatctg
     1021 gtggtgacct tggcgtcatg gcaggaggtg accaggatgt ccaggacatt gccctcccgg
     1081 tcgatcacac accgtaagta gtggatcttg ccgtggactt gatgagcacg agtcaggccg
     1141 tcaaagtgag ttaatcaaca cggccgccac ggtctcggcg acattgaacc gctcaccgag
     1201 accggtagcc tcaagggctc gattggccat tccccggcgg ggaaccacca accagaggta
     1261 gcggttttcc cgggtcgcga gttcagcggc cgcaaacagc acagacaacc cggcggaccc
     1321 caaggatcgc accctctcaa ggtccaccac caccagtgag gcagcggcta gctgggcggt
     1381 cagcaacacg tccagttccg gagcggtcag ggcgtcaatc tcgcctccta cggtgaccac
     1441 ccgaacctgc ggaccgtgtt cggtcacctg cagatccaag acgtcgcagg acaacggtcc
     1501 tgccctgccc agtgtgcgta attgcgccat caccagctcc tttccctgcc gtggtggcgc
     1561 ccgcggggac gccgaggctt cccggccggc ctggcgccgg ctcccgaacg ccgatctgtg
     1621 accgcacggt tgcctgcagt tacccaaccc gctcagaact caaacccttc ggccgtccac
     1681 cgggtgatga ctgaagccac tgcaggctgc tcgcccacac ccacacagct gattcggtgc
     1741 cgacctctag atcagacacc gctgcggtga tcgatgactc cgaggcttgg cgcctacaca
     1801 cccgacatcg ccacaccggt cgccatcggg ctgaccgagg tcactgagtg tgcccaccgt
     1861 acgccccacg gatggccata cgaatcacca catttatgcg tgaactcgaa gacgcgcccg
     1921 catatcaagc aggagcggac gtgcgtcggc gactgggcgc cctttgttgt tacaggtgga
     1981 agctggtgcc gacgctgttg ggatgaatgc cgggccgaag cgggtggaca tggcgtgttg
     2041 agctcgccat ccggtgcagt gtgcggggac gaggacgctg gggtgcaggt cgtcaagcac
     2101 cggtgggatg aggggctcga acacgggtcc gttgagatga aatccgccta gcagagcgta
     2161 caggggctgg tcacccgtga gccgccgggc atagcgggca atgttgacca caccagcgtg
     2221 accgcggcgg cgcatgttct cgaccacacc gtctgggctg gtgccagcgt catagagcaa
     2281 ccgatattcg cgatcgccct tcgccacggc caccagcgcg gaaaatccat gctcggcgat
     2341 aagcgcgtcg ggaacctcgc cgttgtccgt taccgccgtt ggccgcacgg cccagcacct
     2401 agagggacgc ggcgggctgg cccttggttg ggcatgagga tgtcggtgac gttgtccatc
     2461 agcgtggtga tccggacgga atcgacgggc tctaggtcga tctcaacggc cggctcgaag
     2521 tcgtggagca cgggcggcgg cagtctggcc gggctaccgt ccgtgcacac gaggcgagcg
     2581 taacggcaca aagatgcgac gtcacgggta cttgtccccg agtgctacga acatcaacta
     2641 cgcagcacgc gccgtcgcca ccatcggaca ccacgacagc gtgatgtggc ggactgcgag
     2701 ccccggcgca gaccgcggta gccccagaac tgccccggat gcgcatgcgg ggtgaaagct
     2761 ttactggtac
//
LOCUS       DATADN010000764         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_698056, whole genome shotgun sequence.
ACCESSION   DATADN010000764 DATADN010000000
VERSION     DATADN010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698056"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1161)
                     /locus_tag="VHH53_09945"
     CDS             complement(73..1161)
                     /locus_tag="VHH53_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX2290489.1"
                     /translation="MRTRRRWGTIGLAGALGLTFLLLVVIGTVPPDGRSAPAEAAAPA
                     PPPWLRPLAAGELPPQFVLFSFDGAGSHTHWQRVLEASRAVGAHVTGFLSGPYLLPHE
                     RRQDYTGPGHAPGDSAIGFGGSIEEVRTRIGDLNAAVEAGHEIGTHYNGHFCKGMEPS
                     VGTWTAEQWTSELDQFFRFLHDAGGQGLRISGTMIKGGRTPCLEGRFDELLTVLGRRG
                     MTYDASQISDGLAWPEQRHGVWQFWMPLVQIPALNNRKVIMMDYNLWYAMNRAKEDPA
                     RSAEFAAITLDTYRAAYDAAFNGNRAPLVVGNHFNDWAGGAFSSAAERFMAEVCVRPQ
                     TVCATYSEVIDWLLLQDPAVLAELSAAQ"
     gene            1399..>2093
                     /locus_tag="VHH53_09950"
     CDS             1399..>2093
                     /locus_tag="VHH53_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX2290490.1"
                     /translation="MRSTSNPAFRNLPVGGFGGGYAGFTPQAQGYPAEPGYGGYQAPP
                     TTGDRPMTVDDVVTKTAMTLGLALIVGVITAVAVLSETINPFPPMIIGLLAGLVLSLV
                     VIFKKSVSPGLILAYSAAEGLFLGAITGVFEYIYPGIAFQAILGTALVFGTMLVVYRT
                     GAIKVTPKLTKWIIGATIGAAGLMLINLLLSLFGVSTGLREAGPLGIIVSLLFIGIAA
                     FNFLLDFDMADQM"
BASE COUNT          300 a          709 c          736 g          348 t
ORIGIN      
        1 ggcgtggggc aacgaccgcg ccgggatgga cctggccggg caggtggacc cggaattcgg
       61 gtggcccggc ggctactgcg ccgcgctcag ctcggcgagc acggcgggat cctggagcag
      121 cagccagtcg atcacttcgg agtaggtggc gcagaccgtc tgcggccgca cgcacacctc
      181 ggccatgaac cgttccgcgg cgctgctgaa cgcgccgccg gcccagtcgt tgaagtggtt
      241 gcccaccacc agtggggcgc ggttgccgtt gaacgcggcg tcgtacgcgg cgcggtaggt
      301 gtccagggtg atcgcggcga actcggcgct gcgcgccggg tcctccttgg cgcggttcat
      361 ggcgtaccac aggttgtagt ccatcatgat caccttgcgg ttgttcagcg ccgggatctg
      421 caccagcggc atccagaact gccacacgcc gtgccgctgc tcgggccacg ccaggccgtc
      481 ggagatctgg ctggcgtcgt aggtcatgcc ccgccggccg agcaccgtca gcaactcgtc
      541 gaacctgccc tccaggcagg gggtgcggcc acccttgatc atggtgccgg agatccgcag
      601 cccctgcccg cccgcgtcgt gcaggaaccg gaagaactgg tccagctcgc tggtccactg
      661 ctcggccgtc caggtgccga cgctgggttc catgcccttg cagaagtgcc cgttgtagtg
      721 cgtgccgatc tcgtggccgg cctcgacggc ggcgttgaga tcgccgatgc gggtgcgcac
      781 ctcctcgatc gagccgccga agccgatggc cgagtcgccc ggcgcgtggc cggggccggt
      841 gtagtcctgc cggcgctcgt gcggcagcag gtacggcccg gacaggaagc cggtgacgtg
      901 cgcacccacc gcgcgcgacg cctcgagcac ccgctgccag tgcgtgtgcg acccggcacc
      961 gtcgaaggag aacagcacga actggggcgg cagttcgccc gcggccagcg ggcgcagcca
     1021 cggtggcggg gccggcgccg cggcctcggc cggcgcgctg cggccatcgg gcggcacggt
     1081 gccgatgacg accagcagga gaaaggtcaa ccccagcgcg ccggcaaggc cgatggttcc
     1141 ccaccgtctt cgggttctca tgtcgtgtcc ccatggtgtg agctgttgct caacgtagtg
     1201 gtgccggccg gcgttgggcg gcagcgacac gaccgggtac cggccgaacc gggcgtttct
     1261 ccgatcatcc ggcccgcgga ggtctactgt gtctgacgtg cgcgctgttg agtaaagtca
     1321 gctttacccg acgggaaccg gccggcacgg ttggtcgttg gacccgatga aggcgccatc
     1381 aggctccagg aggatcaggt gcgttctacg agcaatcccg cgttccgcaa cctgcccgta
     1441 ggcgggttcg gcggtggcta tgcgggcttc acgccccagg ctcaggggta cccggccgag
     1501 ccgggctacg gcggctacca ggcgccgccg accaccggcg accggcccat gacggttgac
     1561 gatgtcgtca cgaagaccgc catgacgctg ggtcttgcgc tcatcgtcgg cgtgatcacg
     1621 gccgtcgccg tgctgagtga aaccatcaac ccgttcccgc cgatgatcat cgggctgctg
     1681 gccgggctgg tgctctcgct cgtcgtcatc ttcaagaaga gcgtgagccc cgggctgatc
     1741 ctggcctact ccgcggccga gggcctgttc ctcggcgcga tcacgggcgt gttcgagtac
     1801 atctacccgg gcatcgcgtt ccaggcgatc ctgggcaccg cgctggtgtt cggcacgatg
     1861 ctggtcgtct accgcaccgg cgcgatcaag gtgacgccca agctcaccaa gtggatcatc
     1921 ggcgcgacta tcggcgcggc cgggttgatg ctgatcaacc tgctgctgtc gctgttcggc
     1981 gtgagcaccg ggctgcgcga ggccggcccg ctgggcatca tcgtcagcct gctgttcatc
     2041 ggtatcgcgg cgttcaactt cctgctcgac ttcgacatgg cggaccagat gat
//
LOCUS       DATADN010000765         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1007880, whole genome shotgun sequence.
ACCESSION   DATADN010000765 DATADN010000000
VERSION     DATADN010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007880"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1614)
                     /locus_tag="VHH53_09955"
     CDS             complement(277..1614)
                     /locus_tag="VHH53_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2290491.1"
                     /translation="MTAQTAAGRPRLRELRHEAGWTQQQLADKLAHFAWMHDRVHAAV
                     NADMVAKWERGVKGISPRYRALLCQLFGVTAEQLGFSPTPSVAPNQPVRDSESLVSLL
                     DDAANLLDQLGAAGTALAPQMLSAWKDITTSRRTMLGLLDPAATDPAGHARAATATIT
                     DLEQLAQRYQALHATADPAALLTPVAAHIRMATATLGQEHIAPQRRRLWRNLATVATL
                     AGRLACEDLGDAASGRSYYSLAADSAREVDDHQAVATALGYTAQLAHAQGMTAAALNH
                     LSTALTHAEHAPTVASWLATIQATIHADSGDHTAAAHALNRATTSPPVTQSLSLIDHS
                     PAHLLTATGHVHLQAGDHRAAQATLTAAFDQLPSTACRARIITLTDLAMAELRAGNLP
                     DACRHAITAADLLHRTPYVTGATRLRAFRATAARPLDPRTLRALDDHLAHLAA"
     gene            1927..>2220
                     /locus_tag="VHH53_09960"
     CDS             1927..>2220
                     /locus_tag="VHH53_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290492.1"
                     /translation="MGQLGRLVGYSGQHLGAIERGSAVPSEVVVSACDAAVLANGRLV
                     AMLPAVIREQARVRHQHEAARRPARSIDLVGARARQVCDQGDLDWERLADATRR"
BASE COUNT          327 a          619 c          894 g          380 t
ORIGIN      
        1 ctaccgcatc gccctgtacc gcctgcgctg ggatgcccgc acccaggact acctcacccg
       61 ccgcagcacc gaaggcaaaa cccgccgcga agccatccgc tgctaaagcg ctacctcgcc
      121 gcgagatcta ccagatcctc acgacaccac ccgaactgca gccctcagcg gcttgacatc
      181 cataggggca tcagtgcccc tgtgaccttg gggttcagca aggcccgatg cccgttgagt
      241 cttcggcctg caagtcttcg gcggccgatt cgtctgtcag gcggcgaggt gggcgaggtg
      301 gtcgtccagg gcgcgcagtg tccgtgggtc gagggggcgg gctgcggtag cgcggaaggc
      361 gcgcaagcgg gtggcgccgg tgacgtaggg ggtgcggtgg aggagatcag cggcggtgat
      421 ggcgtggcgg caggcgtcgg ggagattgcc tgcgcgcagc tccgccatgg cgagatcagt
      481 cagcgtgatg atgcgggcac ggcaggcggt tgagggcagt tgatcgaacg cggcggtgag
      541 tgtggcttgg gcggcacggt ggtcgccggc ttggaggtga acgtggccgg tggcagtgag
      601 caggtgagcg gggctgtggt cgatcaatga aagcgactgc gtgactggtg gagaggtcgt
      661 ggcacggttg agggcgtggg cggctgcggt gtggtcgccg gagtcggcgt ggatggttgc
      721 ttggatggtc gcgagccagg atgcgacggt gggtgcgtgc tcggcatggg tgagggcggt
      781 ggaaaggtgg ttgagcgcgg cggcggtcat gccttgggcg tgggcaagct gggcggtgta
      841 gccaagcgcg gtggcgacgg cttggtggtc gtcgacttct cgggcgctgt ctgcggccag
      901 gctgtagtag gagcggccgg atgcagcgtc gccgaggtcc tcgcaggcca gacggccagc
      961 gagggtggcc accgttgcca ggttgcgcca cagccgacgc cgctgcgggg caatgtgttc
     1021 ctgacctagc gtggccgtcg ccatccgaat atgtgcggcg accggcgtca gcaacgcggc
     1081 ggggtcggcg gtggcgtgga gggcctggta gcgctgggcg agctgttcca ggtcggtgat
     1141 ggtggcggtg gcggcccggg cgtggccggc ggggtcggtg gcggccgggt cgagcagtcc
     1201 gagcatggtg cggcggctgg tggtgatgtc tttccacgcc gagagcattt ggggggctag
     1261 cgcggtgccg gcggcgccga gttgatcgag caggtttgcg gcgtcatcaa gcagcgatac
     1321 cagcgactcg ctgtcgcgca ccggttgatt gggtgcaacc gaaggagttg gactgaagcc
     1381 gagctgttct gccgtgacgc cgaacagttg gcacagcagc gcgcggtagc gggggctgat
     1441 gcctttgacg ccgcgttccc acttggccac catgtcggcg ttgaccgcag cgtgtacgcg
     1501 gtcgtgcatc caagcgaagt gggccagctt gtcggccaac tgctgctgag tccaacccgc
     1561 ctcatgccgt aactcccgaa gccgcgggcg cccggcagcg gtctgtgcgg tcacagcccg
     1621 ggcaccccct tcgcgatagc gtcaaccagc gggtctggcc ggcatcgggc ggtggcagga
     1681 cggtcggcgt ggactggttg cgggtaagga aacgccccag caggatttgc tcacaacgat
     1741 cagcaaattg gccccttgac tgattcacgg aaggcagccg tagtggacga gccgagtagc
     1801 ggtgagtcgc caggaggcgg caagcggcgc cctgggcgtc cagcaaagat caccgacccg
     1861 gcaagctcgg cggttgcggc gctcggtgct gagctgcggc ggttatgaat cgaccgcggg
     1921 ctgaccttgg ggcagcttgg tcgactggtg ggctactccg ggcagcacct gggcgcgatc
     1981 gaacgcggga gtgcggtgcc ctcggaggtc gtcgtatctg cgtgtgatgc cgcggtgtta
     2041 gcgaatggtc ggttggtggc gatgttgccg gcggtgattc gtgagcaggc gcgggtgcgg
     2101 catcagcatg aagcggccag gcgtccggcc cggtcgatcg atcttgtcgg ggccagggcc
     2161 cggcaggtgt gtgaccaagg cgatcttgat tgggaacggc tggccgatgc gactcggcgg
//
LOCUS       DATADN010000766         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1158250, whole genome shotgun sequence.
ACCESSION   DATADN010000766 DATADN010000000
VERSION     DATADN010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158250"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /gene="rplO"
                     /locus_tag="VHH53_09965"
     CDS             complement(<1..314)
                     /gene="rplO"
                     /locus_tag="VHH53_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX2290493.1"
                     /translation="MAEKKSTKSGGRGRKSSAGAKRAPRVKSQVEATAPAVRALTLSS
                     LSPAPGSTQSRKRVGRGPGSGLGKTSGKGHKGHKARTGGATNPGFEGGQMPMYRRLPK
                     RG"
     gene            complement(314..523)
                     /gene="rpmD"
                     /locus_tag="VHH53_09970"
     CDS             complement(314..523)
                     /gene="rpmD"
                     /locus_tag="VHH53_09970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2290494.1"
                     /translation="MIPTEDAAGSLAVKQIRSEIGHSETMRRTLRALGLRHHQQTVRL
                     PNTASVRGMLTKVRHLVEVSPAQES"
     gene            complement(576..1262)
                     /gene="rpsE"
                     /locus_tag="VHH53_09975"
     CDS             complement(576..1262)
                     /gene="rpsE"
                     /locus_tag="VHH53_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX2290495.1"
                     /translation="MAEETAGTPELEGTSLEGAPAEAQVADRPSGSDRPRGRGGRGGE
                     RGERGGRGGGRRDRGDRERPSDHVENVIAINRVAKVVKGGRRFSFNALVAVGDGIGKV
                     GIATGKANEVSEAVRKAVDAAKRSMIELPRTGSTIPHEVVGEHGAGRVLLKPAATGTG
                     VIAGGPVRAVLECAGITDILTKSLGSTNPHNMVRATMHGLGTLVSARQVARERGVELD
                     QIAYKARQES"
     gene            complement(1264..1635)
                     /gene="rplR"
                     /locus_tag="VHH53_09980"
     CDS             complement(1264..1635)
                     /gene="rplR"
                     /locus_tag="VHH53_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX2290496.1"
                     /translation="MRTIHGPKTRQQLRYRRHLRVRRKVSGTAERPRLVVYRSLKHIY
                     AQLVNDELGVTPLGVSDATEGHQADGAGKVGRAKGTGKLLAQKAKAAGIDKVVFDRAG
                     YRYHGRVQAVADGAREGGLEF"
     gene            complement(1637..2176)
                     /gene="rplF"
                     /locus_tag="VHH53_09985"
     CDS             complement(1637..2176)
                     /gene="rplF"
                     /locus_tag="VHH53_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX2290497.1"
                     /translation="MSRIGRKPVAVPKGVTLQVQGNTVAVKGPRGELRRALHPEMQIA
                     LNNDQFTVARPSDDKRHKALHGLTRTLVQNMVEGVSKGFSKSLEIQGVGYKAEAKPYG
                     VNLIVGYSHPVKYEAPKGIKITVDNNTIVKVEGADKELVGQVAAELRSVRPPEPYKGK
                     GIRYVGEQVRRKAGKTGAK"
     gene            complement(2186..>2393)
                     /gene="rpsH"
                     /locus_tag="VHH53_09990"
     CDS             complement(2186..>2393)
                     /gene="rpsH"
                     /locus_tag="VHH53_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX2290498.1"
                     /translation="LKYHNEAPVIRELRRVSTPGLRRYVKCRELPRVKNGLGMAIVST
                     PRGLMTDREARSANLGGELLAVVW"
BASE COUNT          340 a          863 c          726 g          464 t
ORIGIN      
        1 aatccacgct tcgggagccg ccggtacatc ggcatctgac cgccctcgaa gccggggttc
       61 gttgcgccac cagtgcgagc cttgtggccc ttgtggccct tgcccgaggt cttgcccagt
      121 cccgagccgg gaccgcggcc cacccgcttt cgcgactggg tagacccagg cgcgggtgac
      181 aggctggaca gcgtcagggc gcgaactgca ggcgctgtcg cttccacctg gctcttgacc
      241 cggggagcac gcttggcgcc tgcgctggac ttccggcccc taccccccga cttggtgctc
      301 ttcttctctg ccatcagctc tcctgcgccg gcgacacctc gacgaggtgg cgcactttgg
      361 tcaacatgcc gcgaaccgag gcggtatttg gaagccgcac cgtctgctga tgatgcctca
      421 gacccagtgc gcgcagcgtg cgccgcatgg tctccgaatg gccgatctcc gagcggattt
      481 gtttgaccgc cagagacccc gcagcatcct cggtcggaat cacggctgcg tgatgacccg
      541 gaccttgccg gcctactgct ggattacgtc ccatctcagc tctcctgccg cgccttgtag
      601 gcgatctgat cgagctcgac gccccgctcg cgcgcaacct ggcgggccga caccagcgtg
      661 cctaggccat gcattgtagc gcgcaccatg ttgtgcggat tggtggagcc cagactcttc
      721 gtgaggatgt cggtgattcc ggcacactcg agcaccgccc gcaccggtcc cccggcaatc
      781 accccggtac cggttgcagc gggcttgagc agcacgcggc cggccccgtg ctcgcccacc
      841 acctcatgag ggatggtcga gccggtgcgg ggcagctcga tcatggagcg cttggcggca
      901 tccaccgcct tgcggacagc ctcggagacc tcgttggcct taccggtggc aataccgact
      961 ttgcctatgc cgtcgcccac cgccaccagg gcgttgaagg aaaaccgccg gcctcccttg
     1021 accaccttgg ccacccggtt gatggcgatg acgttctcca cgtgatcgct ggggcgctcc
     1081 cggtctcccc ggtcccgccg cccgccacct cgaccaccgc gctcgccccg ctcaccgccg
     1141 cgaccaccgc gaccacgcgg ccggtccgac cctgatggcc ggtcggccac ctgggcttcg
     1201 gccggcgcac cttccaacga tgtgccttcg agctccgggg tacctgctgt ctcctctgcc
     1261 attctagaac tccaatcccc cttcgcgcgc accgtctgcg acggcctgca cgcgaccatg
     1321 gtaacgataa cccgcccgat cgaagactac cttgtcgatg cctgcggcct tggccttctg
     1381 cgccagcaat ttaccggttc ccttggcccg gcccactttc cctgcgccgt cggcctgatg
     1441 cccttcggtc gcatcgctca cgccgagtgg ggtgacgccg agctcgtcat tgaccagctg
     1501 agcgtagatg tgcttgagcg agcggtacac caccaggcgc ggccgctccg cggtgccact
     1561 caccttgcgg cgcacgcgaa ggtgacggcg atagcggagc tgctgccggg tcttgggccc
     1621 gtgaatcgtg cgcatcttac ttggctcctg tcttgccggc cttgcggcgg acctgctcac
     1681 caacgtaccg aatgcccttg cccttgtacg gctcgggtgg gcgcaccgat cggagctcgg
     1741 cggccacctg ccccaccagc tccttgtccg cgccttcgac cttcactatg gtgttgttgt
     1801 ccaccgtgat cttgatgccc ttgggcgcct cgtactttac cgggtgcgag tagcccacga
     1861 tcagattcac accgtagggt ttggcttccg ccttgtaccc gacgccctgg atctcgaggc
     1921 ttttgctgaa gcccttggac acaccctcca ccatgttctg caccagggtg cgggtaagcc
     1981 catggagagc cttgtggcgc ttgtcatcgc tgggccgggc aacggtgaac tggtcgttgt
     2041 tgagggcaat ctgcatctcg gggtgcagag cacgccgcag ctcgccgcgc ggccccttga
     2101 ccgccaccgt attcccctgg acctggaggg tcactccctt ggggactgca accggctttc
     2161 gaccgatacg tgacatgctg gcctcctacc agactacggc gagcagctcg ccgccgaggt
     2221 tggcgctgcg cgcctcccga tctgtcatca gtccgcgtgg tgtcgagacg atcgccatgc
     2281 ccaggccgtt cttgaccctc ggcagctcgc ggcatttcac atagcggcga agtcccgggg
     2341 tggagacccg gcggagctcg cggatgaccg gcgcctcgtt gtgatacttg agg
//
LOCUS       DATADN010000767         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_405206, whole genome shotgun sequence.
ACCESSION   DATADN010000767 DATADN010000000
VERSION     DATADN010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405206"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..>1514)
                     /locus_tag="VHH53_09995"
     CDS             complement(191..>1514)
                     /locus_tag="VHH53_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HEX2290499.1"
                     /translation="ITAEDFIYNWKVLNGRDCPDCAAFTSSGYDQVQSVVGSDNGKTV
                     TVRMSKPFTDWKQLWSGGSPIYPAHIAAQNGNIKTPEGVAAAFTWFGANVPKYSGGPF
                     QIDNFQNKESVTLVPNRKWYGQTPKLDRLIYRIITDAAQEPTALQNREVQVIYPQPQI
                     DLVQQVRNIPGVSSFVGLGLTWEHFDLNLNNRFLAEPALRTAMFTAIDRQAIIDKTVG
                     QFTDKVAPLNSHNFVPQQSGYEDVVSETGQGSGDLARAKQILAAAGYKIEGGQLRTAT
                     GRVVPPLRIRYTVGNQIRQTECELFAQQVKPLGITVQVVPTDDLGATTSNGDYDIVVF
                     AWVASPFPYGGAAQLWTTGQGNNFGNYSNPDVDRLIAAAASQPDEAQAKEMLNQADRR
                     LTRDAYVLPLYQKPTFIALYDSIANVRNNSSLDGPPYNVAEWGLRAAG"
BASE COUNT          251 a          466 c          495 g          302 t
ORIGIN      
        1 tgctgacagc gggtcaccgg acagcgacac catcacgaac acgacaaacg tcgccatgac
       61 cagaatcggc accgagacga gtatccggcg cactgcgtag acgagcatcg acgcctccct
      121 gcttggagga gatctcctgg cgagcacacc attgaccgct tcgggtcgac gggcgactcg
      181 ccgagtgcga tcagccggcc gcgcgcaagc cccactctgc gacgttgtaa ggcgggccgt
      241 ccagcgacga gttgttgcgg acgttggcga tggagtcgta cagcgcgatg aatgtcggct
      301 tctggtacag cggcagcacg taggcgtcgc gggtcagccg ccggtccgcc tgattgagca
      361 tctccttggc ctgcgcctca tcgggttggc tcgcggctgc ggcgatcagg cggtcgacgt
      421 cgggattgct gtagttgccg aagttgtttc cctgcccggt ggtccacagc tgcgccgcac
      481 cgccgtaagg gaagggactg gcgacccagg cgaacaccac gatgtcgtag tccccgttgg
      541 atgtggtggc gccgaggtca tcggtcggga ccacctgcac ggtgatgccc agcggcttga
      601 cctgctgggc gaacagctcg cattcggtct ggcggatctg gttaccgacg gtgtagcgga
      661 tccgcagcgg cggcactacc cgcccggttg cggtgcggag ctgcccaccc tcgatcttgt
      721 aaccagcggc ggcgaggatc tgcttggccc tcgctaggtc accggaaccc tggccggtct
      781 cgctgaccac gtcctcatag ccgctttgct gcggaacgaa attatggctg ttcagtggtg
      841 cgaccttgtc ggtgaactgg ccaacggtct tgtcgatgat ggcctgccgg tcgatcgcgg
      901 tgaacatggc agtgcgcagt gccggctcgg ccaggaacct gttgttcagg ttcaaatcga
      961 agtgctccca ggtcagcccg agcccgacga aggatgagac gcccgggatg ttgcgcacct
     1021 gctgcaccag gtcgatctgc ggctgcgggt agataacctg gacttcccgg ttctgcagtg
     1081 ccgtcggttc ttgtgccgca tcggtgatga tccggtagat cagccggtcg agcttcgggg
     1141 tttgcccgta ccacttgcga ttgggcacca gggtgaccga ctccttgttc tggaagttgt
     1201 cgatctggaa ggggccgccg gagtacttcg gcacgttggc accgaaccag gtgaacgccg
     1261 cagccacccc ctctggagtc ttgatgtttc cattctgtgc ggcgatgtgc gccgggtaga
     1321 tcggtgagcc gccactccac agctgcttcc agtcggtgaa cggcttggac atcctcaccg
     1381 tcaccgtctt gccgttgtcg ctgccgacga ccgactgcac ctggtcgtaa cccgaactgg
     1441 tgaatgcagc gcagtccggg cagtcgcgtc cgttcagaac cttccagttg tagatgaagt
     1501 cctcagcggt gatc
//
LOCUS       DATADN010000768         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_405504, whole genome shotgun sequence.
ACCESSION   DATADN010000768 DATADN010000000
VERSION     DATADN010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405504"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VHH53_10000"
     CDS             complement(<1..198)
                     /locus_tag="VHH53_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2290500.1"
                     /translation="MTVPSLQSVALQVTELSFAYPDGHRALADINLTVQPGERVALLG
                     PNGAGKTTLVLHLNGVLQPTSG"
     gene            complement(195..959)
                     /gene="cbiQ"
                     /locus_tag="VHH53_10005"
     CDS             complement(195..959)
                     /gene="cbiQ"
                     /locus_tag="VHH53_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX2290501.1"
                     /translation="MGAGHAHPLFLPGNSPVHRLPPHVKVLAAFLFVCCVVATPREAF
                     WAFGGYLLVLVGMWVVAAIPLGWIARRALIEAPFLVLAVLLPFAEGGQRVQVAGVSLS
                     MTGLLAGWNIVVKGTLGVLTSLTLAATTSPRDLLIGLQRLRVPDTVTTIATLMLRYFE
                     VLVGEARRMRIARISRGHDPRLLWQVGATARGVGSLFLRSYERGERVHLAMLARGWNG
                     AMPVVQERLTGRREWLVGLAPVAVAMLLVSGAWWGG"
     gene            complement(966..1349)
                     /locus_tag="VHH53_10010"
     CDS             complement(966..1349)
                     /locus_tag="VHH53_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HEX2290502.1"
                     /translation="MTAPHRQRSLTGFFIAFGVIALLIAGLLSYLASGNPDGLDSATL
                     NGCEVTEVEGTEQLSGQCIAQNARDHDLAGSPLADYALGGDDGLVGIAGVIGVLVTLA
                     LAGGLFWLARPRQGHRTGKRSAGKT"
     gene            complement(1346..>1800)
                     /locus_tag="VHH53_10015"
     CDS             complement(1346..>1800)
                     /locus_tag="VHH53_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX2290503.1"
                     /translation="WLGALCVGIVLVVQALLFADGGLTALGANITNMALIGTWIGFAV
                     AIALRRLVTRSRGGLVTVAFVSALVNTVIASLGFVVEYAIGGAGGSAVGTVAVAILGV
                     HLLIGIGEGIITALTVGAVTSARPDLVYLLRQSPPRTATVATAGRSAS"
BASE COUNT          350 a          620 c          598 g          232 t
ORIGIN      
        1 gccggaagtc ggctggagaa caccgttgag gtgcaggacg agggtggtct tcccggcacc
       61 gttggggccc agcagcgcga cccgctcgcc gggttgcacg gtgaggttga tgtccgccaa
      121 ggcgcggtgg ccgtcgggat aggcaaagct caactcggtg acctgcaacg cgacggactg
      181 caacgatggc acggtcaccc gccccaccag gcaccggata cgagcagcat cgccaccgcc
      241 accggtgcca acccgaccag ccattctcgg cgaccggtaa gtcgttcctg caccaccggc
      301 atcgcgccat tccagcctcg tgccagcatg gccaggtgta cccgttcgcc acgctcgtag
      361 gaccgtagga acaggctgcc caccccgcgt gcggtcgcac ccacctgcca cagcagccgc
      421 ggatcgtgtc cccgggagat tcgggcgatc cgcatccggc gggcttcacc gacgagcacc
      481 tcgaagtacc gcagcatcaa ggtggcgatc gtggtgacgg tgtcgggcac ccgcaagcgt
      541 tgcagcccga tcagcaggtc acgcggcgag gtcgttgcgg ccagcgtcag tgaggtgagt
      601 accccgaggg tgcccttgac cacgatgttc cagccggcaa gcagccccgt cattgacagc
      661 gacacccccg cgacctgcac ccgctgcccg ccctcggcga acggcaacag cacggcaagc
      721 acgagaaacg gtgcctcgat aagagcacgg cgcgcgatcc aacccagcgg aatcgccgcc
      781 actacccaca tcccgaccag gacgaggagg taaccgccga acgcccagaa cgcctcccgc
      841 ggcgtagcca ccacgcagca cacgaacagg aatgcagcga gcaccttaac gtggggtggc
      901 agccggtgca ccggtgaatt gcccggtagg aaaagtggat gtgcgtgacc ggctcccaca
      961 gcggatcagg tcttgccggc cgagcgcttg ccggtcctgt gtccctggcg ggggcgcgcc
     1021 agccagaaca gtccgccggc gagggcgagg gtcaccagca ccccgatgac cccggcgatg
     1081 cccacgaggc cgtcgtcacc gcccagggcg taatcagcaa gggggctacc ggccaagtca
     1141 tgatcgcggg cgttctgggc gatgcactgc ccggagagct gctcggtgcc ctccacttcg
     1201 gtaacctcgc agccgttcaa ggttgccgaa tcgaggccgt cggggttgcc cgaggccagg
     1261 taggacagca gtccggcaat cagaagcgcg atgacgccga acgcgatgaa gaacccggtg
     1321 aggctgcgct gccggtgcgg ggcggtcatg atgcactccg cccggcagta gcgacagttg
     1381 ccgtccttgg cggcgactgc cgcaacagat agacgagatc cgggcgggcg gaggtgacag
     1441 cgccgacggt gagcgcggtg atgatgccct caccgatgcc gatcaggaga tgtactccga
     1501 ggatggccac cgcgacggta cccacagcgg aaccgccggc cccgccgatc gcgtattcca
     1561 cgacgaatcc cagggatgcg atcactgtgt tcaccagggc ggagacgaaa gcaacggtga
     1621 ccagcccccc gcggctgcgg gtaaccagcc ggcgcagggc tatggcgacc gcgaagccga
     1681 tccaggtacc gatcagggcc atgttcgtga tgttggcgcc caacgcggtg aggccaccgt
     1741 cggcgaacag cagcgcctgc accaccagca cgatgccgac acacagcgcc ccgagccagg
//
LOCUS       DATADN010000769         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_80192, whole genome shotgun sequence.
ACCESSION   DATADN010000769 DATADN010000000
VERSION     DATADN010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80192"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VHH53_10020"
     CDS             complement(<1..282)
                     /locus_tag="VHH53_10020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156551.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the delta' subunit seems to
                     interact with the gamma subunit to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX2290504.1"
                     /translation="MSAWDAVVGQDPVVATLREAAQVEAGTMTHAWLFTGPPGSGRSV
                     AARAFAAALQCTDLENGPGCGVCQGCRTTLAGTHADVREVVPEGLSISVA"
     gene            complement(279..1691)
                     /locus_tag="VHH53_10025"
     CDS             complement(279..1691)
                     /locus_tag="VHH53_10025"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2290505.1"
                     /translation="MDPSAEHDLTGSTAGRVRGVLAIPAFRRLWGVTALTATAEWQSL
                     LALSALATQLTSGYQAQSFALGGVVATKLLPALVLGPLAGALADKLDRRHVMVVCDVL
                     RAGLFLSIPLVGELAWLFVATFLIEVCAMFWIPAKDASIPNLLRRRDQVETANQLALI
                     MTYGIAVLVAAGVFTALSKLGPVLSQAGLISGAEGSAYLALTINGIAYAFCALVVGTR
                     IPEISGRALTRRQAQPGVFAMLRDGVVFVRGTPLVRGLVTGIIGAFAAGGAVIACAKL
                     YSTSLGGGDAAYGLLFASLFVGLGVGMAAAPQAARRIRHRRLFGLAIIGAGLALVAVA
                     VVPHLLAALAAVILVGCAAGIAFLTGLTIIGTQVVDEMRGRTIAFIQLLVRLALLASM
                     ALVPLAVGLVQQRTIILFSEQVTIDGTRPVLLCAGLLAATVGLIAHRQMDDRLDGSLR
                     GDVRAALRRNSEPSPPGGGG"
BASE COUNT          377 a          666 c          601 g          246 t
ORIGIN      
        1 ggccactgag atggacaggc cttcgggtac cacctctcgg acgtcggcat gggtgcccgc
       61 cagcgtggtc cggcagccct ggcagacacc acaccccggt ccgttctcca gatcggtgca
      121 ttgcagtgcg gcggcgaagg cccgggccgc aaccgaacga ccgctgccgg gagggccggt
      181 gaacagccag gcgtgagtca tcgtcccggc ctcaacctgg gcggcttccc gcagcgtggc
      241 caccaccgga tcctgaccga ccactgcgtc ccatgcgctc atcccccacc gcccggaggg
      301 ctcggttcgg aattcctacg cagggcagca cgcacatcgc cccgcagcga cccgtcgagc
      361 cggtcgtcca tctggcggtg tgcgatcaag ccaaccgtgg ccgccagcag cccggcgcac
      421 agcagcaccg gacgggtgcc gtcgatggtc acctgctcgc tgaacaagat gatggtgcgc
      481 tgctggacca acccgaccgc cagcggtacc agcgccatcg aggcgagcag ggcgagccgg
      541 acgagtaact ggatgaatgc gatcgtgcgc ccgcgcatct catcgaccac ctgggtcccg
      601 atgatggtca gaccggtcag gaacgcaatc cccgcggcgc accccaccag gatcacggcg
      661 gccagcgcgg ccagcagatg cgggacaacc gccaccgcga ccagggccaa cccggcgccg
      721 atgatcgcga gtccgaacaa ccgccgatgc cggatccggc gggcagcctg gggtgccgca
      781 gccatgccca cacccaaacc gacgaacaat gacgcgaaca gcagcccgta ggcggcgtct
      841 ccaccgccca ggcttgtgga gtacagcttc gcgcaggcga tgacggcgcc gcccgcagcg
      901 aaggcgccga ttatcccggt gaccagacca cgcaccaagg gcgtgccgcg gacgaacacc
      961 acgccgtcgc gcaacatcgc gaacacaccg ggttgagcct gacgccgggt gagggctctg
     1021 ccggaaatct ccgggatccg agtgcccacc accagtgcgc agaatgcgta agcgatgccg
     1081 ttgatggtca gcgccagata agccgatcct tcagcgccgg aaatcagccc ggcttggctg
     1141 agcaccgggc caagcttgga caacgcggtg aaaaccccgg cagcgaccag taccgcaatc
     1201 ccgtacgtca tgatcaatgc aagttgattg gcggtttcca cctggtcgcg gcgtcgcagc
     1261 agattaggga tcgaggcatc cttggccggg atccagaaca tcgcgcagac ctcgatgagg
     1321 aaagtcgcca caaacagcca cgccagctcc ccgaccagcg ggatggacag gaacagacct
     1381 gcccgcagca catcgcagac caccatgacg tgccggcgat ccagcttgtc ggccagcgcg
     1441 ccggccagtg gaccaagcac taacgcgggc agcagcttgg ttgcaactac gcccccgagc
     1501 gcgaagctct gcgcctggta accgctggtg agctgggtgg ccagcgccga aagggccagc
     1561 agggactgcc actccgcggt cgcggtgagc gcggtgacgc cccacaaccg gcggaacgcc
     1621 ggaatggcca gcacgccgcg cacccggccc gcagtggacc ctgtcaggtc gtgctcggct
     1681 gagggatcca tcttgctccg cgtggctcgc tgtggtgaca ccgtactatt cgccccaaaa
     1741 ggggcagcca atctgatgtc acatccagat cgagtcaagc aggtgcgcca gcgccctgcc
     1801 acatcggcac tgcgcgagct gaaagcaaaa agccggagcc aacgtggcct tctcactcag
     1861 gccttcagcg gtggcaggtt accgatgacg
//
LOCUS       DATADN010000770         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1290011, whole genome shotgun sequence.
ACCESSION   DATADN010000770 DATADN010000000
VERSION     DATADN010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290011"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="phoU"
                     /locus_tag="VHH53_10030"
     CDS             <1..540
                     /gene="phoU"
                     /locus_tag="VHH53_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX2290506.1"
                     /translation="ADLVLAELVISGEDEMTALCEEMDQRCVNLLVLQAPVALDLRVV
                     VSAIHAVRDLGRMGNLAQHVAKIARLKHPTVPIPGDVRPMFARMSLLAASLAEEAATA
                     IESQDPMSGNRLAEADDEVDELRRRIFRILFSEDWSHGVEPAVDAALIGRYYERFADH
                     AVAIARQVGFLVTGQPPRR"
     gene            complement(553..1398)
                     /gene="mshD"
                     /locus_tag="VHH53_10035"
     CDS             complement(553..1398)
                     /gene="mshD"
                     /locus_tag="VHH53_10035"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEX2290507.1"
                     /translation="MADLAALLAAAAVCDGVDPISEAASLRLHHPGTGAHLLAHDAAG
                     TLVGYAALDERADRRTAEFAVHPEHRRSGVGGALLAALLDRVSGPLWVWAHGEHPAAL
                     RLAERAGLVRKRELLQLRRGLDQPIPPRALPDGVTLRSFVPGDDEAAVVRVNNRAFSW
                     HPEQSHWDVGALAVREARPWFDPKGFLLAVDQQGRLLGFHWTKVHPEGLGEVYVIGVD
                     PDAQGTGLGAALIAAGMDYLREQGLAEVMLYVESDNATALQTYRKLGFRQHHTDVEFL
                     HVGAS"
     gene            complement(1451..2233)
                     /locus_tag="VHH53_10040"
     CDS             complement(1451..2233)
                     /locus_tag="VHH53_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2290508.1"
                     /translation="MELLLLTADPGPGSVLPALSLLPHVVRPAAPEVAALLDAGPYDV
                     VLVDARSDLAGSRSLCRLLGSTGLDVPVIVVISEGGLVALSADWGSDEILLPTAGPAE
                     VDARLRLLVARRAAEPGAGRSGALVLGELVIDEATYTARLKGRPLDLTYKEFELLKYL
                     AQHAGRVFTRAQLLQEVWGYDFFGGTRTVDVHVRRLRAKLGPEQEQLIGTVRNVGYKF
                     VRPLRSMSSRERAQQHAAEQDAETADSDSDRAAPESLPVQRS"
BASE COUNT          386 a          825 c          759 g          342 t
ORIGIN      
        1 gccgacctgg tactcgccga gctggtgatc tccggcgaag acgagatgac ggcactgtgc
       61 gaggaaatgg accagcgctg cgtcaacctg ctcgtcctgc aggccccggt agccttggat
      121 ctccgggtgg tcgtgtcagc catccatgcg gttcgtgatc tgggccggat gggcaacctg
      181 gcccaacacg tcgccaagat cgcgcggctg aagcacccga ccgtgccgat ccccggcgac
      241 gtccgcccga tgttcgcgcg gatgagcctg cttgctgcca gtctcgccga ggaagccgcg
      301 accgcgatcg agagccagga tccgatgtcc ggtaaccggc ttgccgaggc cgacgacgag
      361 gtcgacgagc tgcgccggcg aatctttcgc atcctcttct cggaggactg gtcacacggc
      421 gtggagccgg cggtggacgc cgcgctgatc ggccgctact acgagcgctt cgccgaccac
      481 gcggtcgcga tcgcccggca ggtcggcttt ctggtcaccg gacaaccacc caggcggtaa
      541 ctcgaccagc ggtcacgatg cccccacatg caggaactcc acgtcggtgt ggtgctgccg
      601 gaaacccagc tttcggtagg tttgtagcgc ggtggcgttg tcggactcga cgtagagcat
      661 cacctccgcg agcccttgct cgcgtaaata gtccatcccc gccgcaatca gtgcggctcc
      721 gaggccggtg ccctgggcat cgggatccac cccgatcaca tagacctcgc cgagtccctc
      781 agggtggacc tttgtccagt gaaagccgag cagccggcct tgctggtcga cggccagcag
      841 gaatcccttc ggatcaaacc acggccgcgc ctcccgcacc gcgagtgcgc cgacatccca
      901 gtggctctgc tcgggatgcc aggagaacgc acggttgttg acccgcacca cggcagcctc
      961 atcgtcgccg ggcacgaagc tgcgcaacgt taccccgtca ggcagggctc gcggcgggat
     1021 cggctgatcc agtccccggc gcagctgcaa cagttcgcgc ttgcggacca gcccggcccg
     1081 ctcggccagc cgcaacgccg ccgggtgttc gccgtgcgcc cacacccaca acggtccgga
     1141 aacccggtcg agcagagcag cgagcagagc accgccgact ccgctgcgcc ggtgctccgg
     1201 gtgcaccgcg aactcggcag tgcgacggtc cgcgcgctcg tccaaggccg cgtaaccgac
     1261 cagggtgccg gcagcatcgt gggccagcag gtgcgccccg gtaccgggat gatgcagccg
     1321 cagcgacgct gcttcggaga tcggatcaac gccgtcgcac accgccgcgg cggccagcaa
     1381 cgcggccagg tcggcgatct gcccctcgga gggccgaagg atccaggtca gcgtcggggc
     1441 cacgcaccgg ttaactccgc tgcacgggaa gtgattccgg tgccgcccga tctgagtcac
     1501 tgtcggctgt ctcagcgtcc tgctcagctg cgtgctgctg ggcccgctcc cgggaactca
     1561 tgctgcgcaa cgggcggacg aacttgtagc cgacgttacg caccgtgccg atgagctgtt
     1621 cctgctcggg gccgagcttg gcgcgcagcc gccgcacatg gacgtcgacc gtgcgggtac
     1681 cgccgaagaa gtcgtacccc cacacctcct gcagcaactg tgcgcgggtg aacacccggc
     1741 cggcatgctg cgcgaggtac ttgagcagct cgaactcctt gtacgtgagg tccaggggcc
     1801 ggcccttgag tcgggcggtg taggtggcct cgtcaatcac cagctcgccg agcaccaggg
     1861 cgccgcttcg gccggcgccc ggctcggcag cgcgccgagc caccagcagc cgcaaccggg
     1921 catcgacttc agccgggccc gcggtgggca gcaggatctc gtcggaaccc cagtctgcgg
     1981 ataatgcgac cagcccacct tcactgatca cgactatcac cggcacgtcc agtccggtgg
     2041 atccgagcaa ccggcacagg ctgcgcgaac cggccaggtc cgagcgggca tcgaccagca
     2101 ccacgtcgta cgggccggcg tccaacaagg cggcgacctc cggcgcagcc ggccgcacga
     2161 cgtgcggtaa caacgacagc gcaggtagca ccgaacccgg tccgggatct gcggtgagca
     2221 gcagcagttc catgcccgct ccctcctgcc gctggaggct ggcggccgcg acctacagca
     2281 cgttctggga ggatacctgg cgttgccaca cc
//
LOCUS       DATADN010000771         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1159240, whole genome shotgun sequence.
ACCESSION   DATADN010000771 DATADN010000000
VERSION     DATADN010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159240"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2200
                     /gene="ileS"
                     /locus_tag="VHH53_10045"
     CDS             <1..>2200
                     /gene="ileS"
                     /locus_tag="VHH53_10045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX2290509.1"
                     /translation="GEFVFYDGPPFANGLPHYGHLLTGYVKDVVPRYQTMRGRRVERR
                     FGWDCHGLPAEVEAERQLGITHKSEIEQMGVADFNAACRTSVLRYTREWRDYVTRQAR
                     WVDFDNDYKTLDLDYMESVLWAFKTLWDKGLIYQGFRVLWYCWRCETPLSNTETRMDD
                     VYRTRQDPAVTVGLRLDSGELALIWTTTPWTLPSNLAVAVHPELTYVTVAAAGERYVI
                     AEDRLPAYAREFGDDPPVLARYLGSELLGRRYTPPFDFFTGRANAHQVLAADYVTTDE
                     GTGLVHLAPAFGEDDKAVTDAAGIEAVVPVDSAGQFTAEVAPYAGMQVFDANRAIIRD
                     LRDGARYVRGVLLRQDTYEHPYPHCWRCNNPLIQRAVSSWFVEVTRFRDRMVALNREI
                     TWVPDNVGDRQFGTWLEGARDWNISRNRYWGSPLPVWISDDPGYPRMDVYGSLDELER
                     DFGVRPVDLHRPFVDQLTRPNPDDPTGRSTMRRVPEVLDCWFESGAMPFAQVHYPFDN
                     ADWFEHHYPGDFIVEYNGQTRGWFYTLHVLATALFDRPAFRTCLAHGIVLGDDGLKMS
                     KSKGNFPEVTEVFNRDGSDAMRWFLMASPILRGGNLVVTEQGIREAVRQALLPLWNSW
                     YFLSLYANAAGVEGRWRTDSPALLDRYVLAKLRELVVDVTALMDRYDIAGACAGVREF
                     CDVLTNWYVRRSRDRFWAGEQDAIDTLHTVLEVTCRVAAPLLPLTTETIWR"
BASE COUNT          355 a          726 c          733 g          386 t
ORIGIN      
        1 ggcgagttcg tcttctatga cggcccccct ttcgccaacg gcctaccgca ctacgggcac
       61 ctgctcaccg gctacgtcaa ggacgtggtg ccgcgctacc agacgatgcg cggccgccgg
      121 gtggagcgcc ggttcggctg ggactgccac gggttgcccg cggaggtgga ggcggaacgt
      181 caactcggca tcacgcacaa gtccgagatc gaacagatgg gcgtggccga tttcaacgcc
      241 gcctgccgca cgtcggtgct gcgctacacg cgggagtggc gcgactatgt cacccgccaa
      301 gcccgctggg tggacttcga caacgactac aagaccctcg acctggatta catggaaagc
      361 gtcctgtggg cgttcaagac gctgtgggat aagggattga tctatcaggg tttccgggtg
      421 ctgtggtact gctggcgctg cgagacaccg ctgtccaaca ccgagaccag gatggatgac
      481 gtctaccgca ctcggcagga cccggcggtc accgtcgggc tgcgcctcga tagtggtgaa
      541 ctggcactga tctggaccac cacgccgtgg accttgccgt ccaacctggc ggtggcggtg
      601 catccggaac tcacgtacgt caccgtggcg gcggccggtg agcgctacgt gatcgccgag
      661 gaccgcttgc cggcctacgc tcgcgaattc ggggacgatc cccccgtgct ggcccggtac
      721 ctggggtcag agctgctcgg ccgtcgctac accccgccgt tcgacttctt caccggcaga
      781 gccaacgccc accaggtgct cgctgccgac tacgtgacca ccgatgaggg caccggtctg
      841 gtgcacctcg ctcccgcctt cggcgaggac gacaaggccg tcaccgatgc cgcgggtatc
      901 gaggcggtgg tcccggtgga ctcggccggg cagttcaccg ccgaggtggc tccgtatgcc
      961 gggatgcagg tcttcgacgc caaccgggcg atcatccggg acctgcgcga cggcgcccgc
     1021 tacgtccgcg gcgtgctgct gcgtcaggac acctacgagc acccctaccc gcactgctgg
     1081 cgttgcaaca acccactgat ccagcgcgcg gtgtcgtcct ggttcgtcga ggtaacccgg
     1141 ttccgggatc ggatggtggc gctgaaccgt gagatcacct gggttccgga caacgtcgga
     1201 gaccggcagt tcggcacgtg gctggagggc gcccgagact ggaacatctc ccgcaaccgg
     1261 tactggggct ccccgttgcc ggtctggatc tccgacgatc ctggctaccc gcggatggac
     1321 gtctacggat ccctcgacga gctggaacgc gacttcgggg tgcgcccggt ggacttgcac
     1381 cgcccgttcg tcgatcagct gacccggccc aacccggacg atccgaccgg gcgctccacc
     1441 atgcgccggg tgccggaggt gctggactgc tggttcgagt ccggcgccat gcccttcgcg
     1501 caggtgcatt acccgttcga caacgccgac tggttcgaac accactatcc gggtgatttc
     1561 atcgtcgagt acaacgggca gacacggggg tggttttaca cgctgcacgt actggccacg
     1621 gcattgttcg accgaccggc gtttcgcacc tgcctggcgc acggcatcgt gctcggtgac
     1681 gacgggctca agatgtccaa gtccaagggc aacttccccg aggttacgga ggtgttcaac
     1741 cgggacggct cggacgcgat gcgctggttc ctgatggcct caccgatcct gcggggcggg
     1801 aacctggtgg tcaccgagca gggcatccgg gaggccgtcc gccaggcgct gctgccgctg
     1861 tggaactcgt ggtacttcct ttcgttgtac gccaacgccg cgggtgtcga aggccggtgg
     1921 cgaacggaca gcccagccct gttggacagg tatgtgctgg ccaagctgcg ggaactggtc
     1981 gtcgacgtca cggcgctgat ggaccgctac gacatcgcag gagcctgcgc cggtgtgcgt
     2041 gaattctgtg acgtgctcac caactggtac gtgcgccggt cccgggaccg tttctgggcc
     2101 ggcgagcagg acgcaatcga cactctgcac accgttctcg aggtgacctg ccgagtcgct
     2161 gcaccgttgc tgccgctgac caccgaaacg atctggcggg
//
LOCUS       DATADN010000772         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1008937, whole genome shotgun sequence.
ACCESSION   DATADN010000772 DATADN010000000
VERSION     DATADN010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008937"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..1325
                     /locus_tag="VHH53_10050"
     CDS             438..1325
                     /locus_tag="VHH53_10050"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2290510.1"
                     /translation="MLVNPNSGVKAGIPTNTAAEEEVRIAVEQCFPGLGENFVVTESE
                     DEAIAATRDAVAKGYEIVVAAGGDGTVGTVACELLGKETALGILPLGSVMNVARMLDI
                     PRDLEGAAAIIATGEVRAIDVGEAKGQIFFEGGSVGLNAAVFREAQQADSGGGRYRAL
                     LAALWVLLRYRPPRMVLHLDDRVLTTRALAVSVANGPYSGLGFTVSPDATPDDGKFDV
                     VIFSRFSRTELIRHFVSIAFGRRQYSAKTATYRTSRVRIEGVHPLPCRADGHDLGWTP
                     VDYVIRPRALRVVVPRSAT"
BASE COUNT          265 a          510 c          529 g          255 t
ORIGIN      
        1 ggcggccagg atctcggcga cgcgagcggg ggtccagtca ccggggttct cgacgcccat
       61 ctccagcaga tcgaaaccca tcgccttcac ccgcggcgca atctccgcga tcgccgcatc
      121 ggtcgacggc gaaacccaga cccaggaatt gacgccgatt cgattcatgg agcggctctc
      181 acgattcgcg atgaccagcg gccagtggcg caccctacgc aagctctgga acagtgtcaa
      241 gcgctcatgc acgctctgac accggaccgg gtaccgttcg cggggcaaac actgggccga
      301 cagccgccgg cgccgcgggg tgtccaccaa ctccgagggt ggccggggtc acgatcgcat
      361 ctgaatcgag gactgatagc gagatgacgg cacgaatcga cgagtccagc acggccggga
      421 ctcaacggcg gattcgtgtg ctggtcaacc cgaactcggg cgtgaaagcc ggcatcccga
      481 ccaacaccgc ggcggaagag gaagtgcgga tcgccgtgga acagtgcttc ccggggcttg
      541 gtgaaaactt cgtggtgacc gagtcggagg atgaggcgat cgccgccacc cgcgacgcgg
      601 tagccaaggg ctacgagatc gttgttgccg ccggtggcga cggcacggtc ggcacggtcg
      661 catgcgagct gctggggaaa gagaccgcgc tcgggatctt gccgctcggc agtgtcatga
      721 acgtggcgcg catgctcgac atcccgcggg atctcgaggg cgcagccgca atcatcgcca
      781 ccggcgaagt gcgcgcgatc gacgtcgggg aggcaaaggg gcagatcttt ttcgagggcg
      841 ggtccgtggg gctgaacgcc gcagtcttcc gcgaggcgca gcaggccgac agcggcggcg
      901 ggcgctaccg ggcgctgttg gcagccctgt gggtccttct ccgctaccgg ccgccgcgga
      961 tggtgctcca tctcgacgac cgggtcctca ccacccgcgc cctggcggtt tccgtggcga
     1021 acggccccta ctccgggttg gggttcaccg tatcaccgga cgcaacgccg gatgacggca
     1081 agttcgacgt ggtgatcttc tcccgcttct cccgcacgga gctgatccgg cacttcgtct
     1141 ccatcgcctt cgggcgccgg cagtactccg cgaagaccgc cacctatcgg acgtcacggg
     1201 tccggatcga gggggtgcac ccgcttccct gccgcgccga tggccatgat ctggggtgga
     1261 caccggtaga ctacgtcata cgaccacggg ctttgcgtgt ggttgtgcca agatcagcca
     1321 cataacggta ggccgtgcca gggggcaacg tttttccacg caaccggccg ccctggtgac
     1381 cggttgcgtg gagcgtcact gacgactcgt tcagatgagc tcaggcgtag taacggtgcc
     1441 cgcgccgccc cctgtcgctc aggcggccca tgctcccgcg acttgcctgg ttgccgcgtt
     1501 gtggcggttc cagacgttga tctgagcgat ctggagaatc aaggcggcac gcgccggct
//
LOCUS       DATADN010000773         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1008958, whole genome shotgun sequence.
ACCESSION   DATADN010000773 DATADN010000000
VERSION     DATADN010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008958"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1547)
                     /locus_tag="VHH53_10055"
     CDS             complement(<1..>1547)
                     /locus_tag="VHH53_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005446213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEX2290511.1"
                     /translation="RRVHLDHDPAAADIPRATLDPTAERRIADAAAHRRRVADSLAQQ
                     YSDDWREVAPDLPGAQRCAATVALLHQGAPFVWGGLLPADPLGGRRGGADLLVRHRGG
                     YLPVIVVRHKVTDPGSGARTSPLTQPILAAARTDRARKVRPQSRDQLRLAHTFRLLQA
                     AGLAPRGRATGGVVGLEADVVVWHDLDAPTWPAGRTAMAEYDARFADRLAVARAAATE
                     AQPLALPSRVTECRSCPWWPTCGPTLRASRDVSMVLRGEDTVALQAAGVSTVDALAAL
                     DPAGEPPVPMVGMPLRDAVLLARAWQRDLTLVRRNQHICVQRADVEVDVDMESFDEAG
                     AYLWGCLLSGSDIGLSGGYRAFATWEPVPTPDEARSFAAFWGWLVDVRSRAALRGLSF
                     RAYCYNEQAENRWMLSSAQRFAGAPGIPTVTEVQEFISSDQWVDLYELVSSEFLCAHG
                     KGLKTIAPAAGFSWHDPEASGENSMRWYRDAVGLGGDEPDPAQRERLLTYNADDVRAT
                     RALRLWM"
BASE COUNT          242 a          547 c          538 g          220 t
ORIGIN      
        1 ctcatccaca gtctcaaggc gcgggtggcg cgcacgtcat cggcgttgta cgtgagcaat
       61 ctttcgcgct gcgccggatc cggctcatcg ccacccagcc cgaccgcgtc ccggtaccag
      121 cgcattgagt tctccccgct ggcttcggga tcgtgccagg aaaagccggc tgccggagcg
      181 atcgtcttga gccctttgcc gtgcgcgcac aggaactcgc tgctgaccag ctcgtacagg
      241 tccacccact ggtcggagga gatgaactcc tgcacctcgg tgacggtcgg gattcccgga
      301 gcaccagcga agcgctgcgc ggaggacagc atccagcggt tctcggcttg ctcgttgtag
      361 cagtaggcgc ggaagctcaa gccgcgcagc gcagcccggc tgcgaacgtc gaccagccag
      421 ccccagaacg cggcgaacga tcgcgcctcg tctggtgtcg gtaccggctc ccaggtggcg
      481 aaagcgcggt agccgccgga cagtccgatg tcggagcccg acagcaggca tccccacagg
      541 tacgcgccgg cttcgtcgaa actctccatg tccacgtcga cctcgacgtc ggcacgctgc
      601 acgcagatgt gctggttacg ccgcaccagc gtgaggtctc gctgccaggc acgggccagc
      661 aggaccgcgt cgcgcaaggg catcccgacc atcggcaccg gcggctcgcc cgccgggtcc
      721 agggcggcga gagcgtctac ggtggatacc cccgccgcct gcagcgccac cgtgtcctcg
      781 ccacgcagca ccatgctgac gtcccgcgac gcccgcagcg tcggcccgca ggtgggccac
      841 cacgggcatg aacggcattc ggtgacccga gatggcagtg ccagcggctg cgcttcggtg
      901 gccgcagcgc gcgccacagc cagccgatcg gcgaaccgag catcgtattc ggccatcgcg
      961 gtacggccgg ccggccacgt gggggcgtcc aggtcgtgcc agacaaccac atcggcctcc
     1021 agaccgacca cgccgccggt ggcccgaccg cgaggcgcca gtccggccgc ctgtagcagt
     1081 cggaaggtgt gtgccagccg taactggtcc cgggattgcg ggcggacctt gcgggcccgg
     1141 tcggtacggg cggcagccag gatgggttgg gtcagtgggc tggtccgggc gccggagccc
     1201 ggatcggtga ctttgtgccg cacgacgatc actggcaggt aaccaccgcg gtgacgcacc
     1261 agcaggtcgg cgccgcctcg tcgcccgccg agtgggtctg ccggcaacag cccgccccag
     1321 acgaacggtg caccctggtg cagcagcgca acagtggccg cgcagcgctg cgcaccaggg
     1381 aggtcgggcg caacctctcg ccagtcatcg gagtattgct gggccagact gtcggcgacc
     1441 cggcgacggt gcgcggcggc atcggcaatt cgccgctcgg cggtggggtc gagggtcgcc
     1501 cgtgggatgt cagctgctgc cgggtcgtga tccaaatgca cccggcg
//
LOCUS       DATADN010000774         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1290268, whole genome shotgun sequence.
ACCESSION   DATADN010000774 DATADN010000000
VERSION     DATADN010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290268"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VHH53_10060"
     CDS             <1..1448
                     /locus_tag="VHH53_10060"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2290512.1"
                     /translation="EDVGKRYEAYGWHVQHVDDGEDLAALDAALAAAKAETTRPSMIV
                     LRTVIAYPAPNKMNTGAAHGSALGDDEVAAVKQTLGFDPQSSFVVEPEVLEHTRQAVT
                     RGQAAHAQWQQSFDSWRQANASGAALFDRLIRRELPSGWAEKLPTWQPDPKGVATRKA
                     SGEVISAIGEVLPELWGGSADLGESNNTTIKGADSFGPEKISTKMWTAQPYGRTLHFG
                     VREHAMGAILNGIVLHGPTRPYGGTFLIFSDYMRPAVRLAALMQAPTIYIWTHDSIGL
                     GEDGPTHQPVEHLAALRAIPGLDVVRPADANETAAAWKAVLEKGERPTGLALTRQSVP
                     VLEGTDAAGVARGGYVLSDTAPDVGGGLPDVLLIATGSEVQFAVAAQQTLAAEGTSAR
                     VVSMPCVEWFDAQGQAYCDQILPPSVKARVAVEAGVAMPWHRFVGDHGEVVSLEHFGA
                     SADWQTLFREFGFTGDAVADAARRTLGRIPK"
     gene            1478..2605
                     /gene="tal"
                     /locus_tag="VHH53_10065"
     CDS             1478..2605
                     /gene="tal"
                     /locus_tag="VHH53_10065"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX2290513.1"
                     /translation="MNSVDTVQALSEAGVSIWLDDLSRQRINSGNLADLIRERHVVGV
                     TSNPTIFASAVTHADDYAAQVRELAARGASVDEAVREITVADVQLACDVFSGTWETTD
                     RVDGRVSLEVSPQLAHDTDGTLAQAAELWKVVDRPNLMIKIPATAEGLPAISGTLADG
                     ISVNVTLVFSVQRYREVIGAFFAGLERAMDNGHDLPTITSVASFFVSRVDTEVDKRLE
                     AIGTEQALALRGKAAVANARLAYAAYQQEFATPRWTRLQKAGARTQRPLWASTGVKNP
                     TYPDTKYVTELIAPGVVNTMPEKTLFALVDHGEVPGDQVSGTAEQAQRIFDELADVGI
                     DLDDVFVVLEREGVQKFDASWVELGETVAAQLAKAAPQPGA"
     gene            2612..3691
                     /locus_tag="VHH53_10070"
                     /pseudo
     CDS             2612..3691
                     /locus_tag="VHH53_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
     gene            complement(3654..>3870)
                     /locus_tag="VHH53_10075"
     CDS             complement(3654..>3870)
                     /locus_tag="VHH53_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX2290514.1"
                     /translation="VVEGPAAPGFADAGIDAIPVAIGPGVETAAVAVTGTLGEQLLLW
                     EHATAVVGRLLGINPFDQPDVEAGRTG"
BASE COUNT          608 a         1309 c         1373 g          580 t
ORIGIN      
        1 ccgaggacgt cggcaagcgg tacgaggcct acggctggca cgtccagcac gtcgacgacg
       61 gcgaggatct cgccgctctc gacgccgccc tcgccgccgc caaggccgag accacccgcc
      121 cgtcgatgat cgtgctgcgc accgtgatcg cctacccggc accgaacaag atgaacaccg
      181 gtgccgcgca tggcagcgca ctgggcgacg acgaggtcgc tgcggtgaag caaaccctgg
      241 gcttcgaccc gcagagctct ttcgtcgtgg agcccgaggt gcttgaacac acccggcagg
      301 cggtgacccg cgggcaggcc gcgcacgcgc agtggcagca gtccttcgac tcctggcggc
      361 aggccaacgc ttccggcgcc gcgttgttcg atcgcctcat ccgccgggaa ctaccctccg
      421 gctgggcgga gaagctgccc acctggcagc cggaccccaa aggggtggct actcgcaagg
      481 cgtcgggtga ggtgatttct gcgatcggcg aggtgctgcc ggagctgtgg ggtggctctg
      541 cggacctcgg tgagagcaac aacaccacga tcaagggggc ggattcgttc ggtcctgaga
      601 agatctccac gaagatgtgg accgcgcagc cctacgggcg cacgctgcac ttcggggtgc
      661 gtgagcatgc catgggcgcg atcctcaacg ggatcgtgct gcacgggccg acccggccct
      721 acggtggcac tttcctgatc ttcagcgatt acatgcgccc ggcggtgcgg ctggcggcgc
      781 tgatgcaggc gccgaccatc tacatctgga cgcacgactc catcggactc ggcgaggacg
      841 gtccgacgca ccagccggtc gagcatctgg ccgcgctgcg ggccatccct gggctggacg
      901 tggtgcggcc cgcggacgcc aacgagacgg cggcggcatg gaaggcggtg ctggagaagg
      961 gtgagcggcc taccgggctg gccctgaccc ggcagagcgt gcccgtcctg gagggcaccg
     1021 acgccgcggg ggtcgcccgc ggcggctacg tgctctccga caccgcgccc gacgtcggcg
     1081 gcgggctgcc cgacgtgcta ctgatcgcca ccggctccga agtgcagttc gccgtcgccg
     1141 cccagcagac cctggcggcc gagggaacca gcgctcgggt ggtgtcgatg ccgtgcgtgg
     1201 agtggttcga cgctcagggt caggcctact gcgaccagat actccctccc tcggtgaagg
     1261 ctcgggtggc cgtcgaagcc ggcgtcgcca tgccgtggca ccgtttcgtc ggtgatcacg
     1321 gtgaagtggt gtcgctggag catttcggtg cctccgccga ctggcagacc ctgtttcgcg
     1381 agttcggctt caccggtgat gcggtagccg acgcggcccg ccgtacgctg ggtcggatcc
     1441 cgaaataaca atcctgacga atcgggagcg agccctcatg aacagcgtcg ataccgtgca
     1501 ggccctgtcc gaggcagggg tgtcgatctg gttggacgat ctgtcccggc agcggatcaa
     1561 ctcgggcaac ctggccgact tgatccggga gcggcacgtg gtcggggtga ccagcaaccc
     1621 gaccatcttc gccagtgcgg tgacccacgc tgacgactac gccgcgcagg tgcgcgagct
     1681 ggcggcccgc ggtgcctcgg tcgacgaggc ggtgcgtgag atcaccgtgg cggacgtgca
     1741 gctggcctgc gacgtgttca gcgggacctg ggagaccacc gaccgggtgg acggccgggt
     1801 gtcgctggaa gtcagccccc agctggcaca cgacaccgat ggcacccttg ctcaggccgc
     1861 agagctgtgg aaggtggtcg accgacccaa cctcatgatc aagattccgg cgacggcgga
     1921 gggcctgccg gccatcagcg gcacgctggc tgatggcatc agcgtgaacg tgacgttggt
     1981 gttctcggta cagcgctacc gcgaggtgat cggtgcgttc ttcgccggcc tggaacgcgc
     2041 catggacaac gggcacgacc ttccgacgat cacatcggtt gcctcgtttt tcgtctcccg
     2101 agtggacacc gaggtggaca agcggctgga ggcgatcggc accgagcagg cactggcgct
     2161 gcggggcaag gccgcggtgg ccaacgcccg gctcgcctac gcggcctacc agcaggagtt
     2221 cgcgacgcca cgctggactc gtctgcagaa ggctggggcg cgcacgcagc ggccgctgtg
     2281 ggcgtcgacc ggggtgaaga accctaccta tccggacacc aagtacgtca ccgaactgat
     2341 cgcgccgggg gtggtgaaca ccatgccgga gaagacgctg ttcgccctag tcgaccacgg
     2401 cgaggtgccc ggcgatcagg tgtcgggtac tgccgagcag gctcagcgca tcttcgacga
     2461 gctggctgac gtcgggatcg acctagacga cgtgttcgtc gtactcgagc gcgagggtgt
     2521 gcagaaattc gacgcatcct gggtcgagct cggcgagacg gtggccgcgc aactggccaa
     2581 ggccgcacca cagccgggag cctgaccgcc gatgccgcag ctcgccgtcg acatccgctc
     2641 ctccgccctg gcggcgcaag cgcggtcctc cctcgacaag ctcgtcgagc aacaggtcgc
     2701 ctcggctctc gccggccaag atcccacgct gtggggcccg caggccgaac ccgaggctgc
     2761 ccggcggctc gcctgggtgg cacttcccga gacctcacgg cccctgctgg cgataattga
     2821 tgcgctgcgg gccgacttgc acgccgatga catcgaccgt gtcgtgctgg ccgggatggg
     2881 tggctcgtcg ctggccccgg aggtcatctg cgccactgcc aaggtggcgc tcacggtgct
     2941 ggacaccacc gacccggagc aggtagcaga tgcgctggcc ggagacctgg agcgcaccgt
     3001 ggtggtggtg tcatcgaaat cagggtccac gatggagacc gagagccacc gtcgggtttt
     3061 caccgcggcg ttcaccgcgg ccggggtgga tccggcggcg catctggtgg tggtgaccga
     3121 ccccggatcg ccgctgcagc agatcgccga cgctgagggc taccgcgcgg tgatcaccgc
     3181 caacccggac gtgggcgggc gctactcggc gctgaccgcc ttcggcctgg taccggcggg
     3241 ccttgccggg gcggacatcg ccggcctgct ggatgacgcg gcgcaggtgg cgacgctact
     3301 ggccgccgac gacccggcca atccagcgct gcagctggcc gcggcgctgg ctgccgggca
     3361 taccaccggt gccgacaagg tggtactcgc cgacaccggc tcgggaatcg tcggcttcgg
     3421 tgactgggcc gagcagctca tcgccgaatc caccggcaag gacggcaccg ggctgctgcc
     3481 ggtcgtggtg gagggaccgg ccgcgccggg ctttgccgac gccggacccg atgccatccc
     3541 ggtcgcgatc ggacctggtg tcgagaccgc cgcagtggcg gtcaccggca ccctgggtga
     3601 gcagttgttg ctctgggagc acgccaccgc ggtggtcggc cggctgctgg gaatcaaccc
     3661 gttcgaccag cctccacgtc aggctggtcg aacgggttga tccccagcag ccggccgacc
     3721 accgcggtgg cgtgctccca gagcaacaac tgctcaccca gagtgccggt gaccgccact
     3781 gcggcggtct cgacaccagg cccgatcgcg accgggatgg catcaattcc ggcgtcggca
     3841 aagcccggcg cggccggtcc ctccaccacg
//
LOCUS       DATADN010000775         4394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_552743, whole genome shotgun sequence.
ACCESSION   DATADN010000775 DATADN010000000
VERSION     DATADN010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552743"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1040
                     /locus_tag="VHH53_10080"
     CDS             132..1040
                     /locus_tag="VHH53_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2290515.1"
                     /translation="MTDSRERVALACFVAGAVLAGGNGVSIRFSNRELAPLWGAGLRF
                     ALAAALLLAVMAVRRLALPHGRALTGALLYGALNFGAAFALAYYALVRLHAGLGQTLL
                     ALVPLATLLLAVAQHQERLRAAAVAGTLLALAGVAVISQGSVGASVPLASLLAAVASA
                     ICIAQAAVLVRRFPPVHPVTMNAVGMTTGAVLLLAGSVLGGEPHRLPQRAATWAAVGY
                     LVVIGSVVVFVLYLVVLRYWAASRAAYGFVLIPFVTVVLSAWLDDEPLGVGLGVGGLL
                     VLAGVYVGALRPTGPPPTPGGTAPRR"
     gene            1148..1606
                     /locus_tag="VHH53_10085"
     CDS             1148..1606
                     /locus_tag="VHH53_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX2290516.1"
                     /translation="MDTVNARGALVGGWRLVSWENRATDGQVTYPMGIDALGALLYTA
                     DGRFSVTISRRGREGFAAGDLLSGTGEEKARAVEGFVAYAGRYSFHGDRVIHHVELSL
                     FPNWVGSDQERWVELAGNRLILSASPLLLAGKLQVPRLVWERVDSSPNRG"
     gene            1835..2524
                     /locus_tag="VHH53_10090"
     CDS             1835..2524
                     /locus_tag="VHH53_10090"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX2290517.1"
                     /translation="MIDPPAAAAAWAALEQPWQLAFELAWEAFQAGSVPVGAVVVDPG
                     GDLVTQGRSRSNEPTGPSGQLAGTYLAHAEVNALAGLAPGDYWSHVLYTTLEPCLLCT
                     AALTHAHVGAVRYAASDPLWAGIERLPRLNQHIAGRWPDRQGPLSGPLAGWGGLLPLM
                     WSLARAADGPVARAYEQTAPPLLQLARVLVGSGEIVALRQLPLAQALHKIWPRLLTLQ
                     RHVGRKADHQH"
     gene            complement(2760..3605)
                     /locus_tag="VHH53_10095"
     CDS             complement(2760..3605)
                     /locus_tag="VHH53_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsnF/AvaK domain-containing protein"
                     /protein_id="HEX2290518.1"
                     /translation="MADIGTVHGWRGHTMMDRDGGLIGRIDAIYVDDRSGQPEWALVS
                     LGAAGTTSTFVPIAQATVSGSEVRVPYDRQLVKDAPGTPLDRPLSVAEERQLFRHYGL
                     DYDASHAGTPRQGPGGTTDAALPASAREAGQDTTEPASNPGRDTRQAASGQMGDDAMT
                     RSEEELRVGTQTRERGRVRLRKYVTTEEQQVTVPVRREEVRLEHEPPADANVDAATSD
                     PKATDAGHEVVLHEEQPVVDKRVVPKERVRLDKETVTDQQQVTEEVRKEQIDVQDHSR
                     SNRRP"
     gene            3956..4231
                     /locus_tag="VHH53_10100"
     CDS             3956..4231
                     /locus_tag="VHH53_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290519.1"
                     /translation="MTPGAAYRRTRSRGVEIVDGATVSVAVVASRTEAELIVGMLRTH
                     GVSATVSADDAGGQYPQMQMDGVRVLVAPADEASARRLLDTAGDTLE"
BASE COUNT          552 a         1527 c         1557 g          758 t
ORIGIN      
        1 cccgtagtca ctcccgcccc ctcgtgttgc ggcgttcgtt acctcggcgc ccgtacggca
       61 gaaccgtcgt tcaccgggct tggcttggtg gaggggttgg gaatcggatt accgcagcgt
      121 agcgttgacc aatgaccgac agccgtgagc gggtggcgct cgcctgtttc gtggctggcg
      181 ccgtgctcgc cggcggcaac ggggtctcca tccggttcag caaccgcgag cttgcccccc
      241 tatggggcgc cggcctccgc ttcgccctgg ccgccgccct gctgctggcg gtcatggcgg
      301 tgcggcggct ggcgctcccc catgggcggg cgctgaccgg tgccctgctg tacggcgcct
      361 tgaacttcgg ggccgccttc gccctggcct actacgccct ggtccggctg cacgccgggc
      421 tcggccagac cctcctggcc ctggtcccgc tggccacgct gctgctggcc gtggcccagc
      481 accaggagcg cctgcgggcc gcggcggtcg ccggcacatt gctcgcgctg gccggggtcg
      541 ccgtcatctc gcagggatcg gtgggggcat cggtcccact ggcctcactg ctggcggcgg
      601 tggccagtgc gatctgcatc gcccaggcgg cggtgctggt gcgccggttc ccgccggtcc
      661 atccagtgac catgaatgcg gtcggcatga ccaccggcgc agtactgctg ctggctggat
      721 cggtactggg tggcgagccg caccgacttc cgcagcgggc ggcgacctgg gcagccgtcg
      781 gctacctggt ggtgatcggc tcggtggtgg tgttcgtgct gtacctggtg gtgctgcgct
      841 actgggccgc atcgcgggcg gcgtacgggt tcgtgctcat cccgttcgtg accgtggtgc
      901 tgtcggcctg gctggacgac gagccgctcg gcgtggggct gggggtcggc gggctgctcg
      961 tactggccgg cgtgtacgtc ggcgccctgc gtcccacggg tccgccgccc acgcctggcg
     1021 ggaccgcacc tcgacgctga gctaggaaca gcacaccacc agcggaatga cgttgtgtcc
     1081 cctcccagca cctccggccc gcctctcagc ctcgttagga tctgggcaga ctcgcccggg
     1141 aggccccatg gacacggtca acgcgcgcgg cgcgctggtt ggaggctggc ggttggtctc
     1201 ctgggagaac cgggccactg acggccaggt cacctacccc atggggatcg acgccctcgg
     1261 cgcgctgctt tacaccgccg acggccgctt ctcggtcacc atctcgcgga ggggccggga
     1321 gggcttcgcc gccggcgacc tgctcagcgg gaccggcgag gagaaggccc gggccgtgga
     1381 gggctttgtc gcctacgcgg gccgctacag cttccatggc gaccgggtca tccatcatgt
     1441 ggagctgtca ctgttcccca actgggtcgg cagcgaccag gaacgatggg tcgagctcgc
     1501 cgggaatcgg ctgatcctga gtgccagccc cctgctgctg gcgggcaagc tgcaggtacc
     1561 tcgcctggtc tgggagcggg tcgactcatc tccgaaccgc ggctgatcgc ttacctggca
     1621 ggttcgacgg atcgcaaggt gccgccaggc ggccaagagc cgtcagtgag ccaccattcg
     1681 gccgacaagc cctcagccaa ccggcagatc cgtaggctgg gtgcccgcgt tggactagtc
     1741 gggtccagtc gggtttacct tgctctggtt gggcggctcg tcggtcgagc aggatcgaga
     1801 tggaacccgg agatcgttcg aatgctcaca ggggatgatt gacccgcctg cggctgcagc
     1861 tgcatgggcg gctctagagc agccctggca gcttgccttc gagctggcct gggaggcctt
     1921 ccaagccggc agcgtcccag tcggggcagt cgtggtcgac cccggcggcg acctcgtcac
     1981 ccaagggcgc agccgcagca acgagcccac cgggccgtcc gggcagcttg ccgggaccta
     2041 cctcgcccat gccgaggtca acgcactagc cggtctcgca cccggggact actggtccca
     2101 tgtgctctac accaccctgg aaccgtgcct gctgtgcacg gcggcgttga cccacgccca
     2161 tgtcggcgcg gtgcgctatg cggccagcga tccgctgtgg gccgggatcg agcggctccc
     2221 gcggctcaac cagcacatcg ctgggcgctg gccggaccgc caggggccac tatcagggcc
     2281 gcttgccggc tggggcggcc tcctgccctt gatgtggagt ctggcacgcg cagcggacgg
     2341 cccggtcgcg cgtgcctatg agcagaccgc gccgccgctg ctgcagctcg cccgggtgct
     2401 ggtcggctcg ggggagatcg tcgcgcttcg ccagcttccg ctcgcccagg cactgcacaa
     2461 gatctggcct cgactgctga cgctccagcg tcatgttggc cgaaaggctg accaccagca
     2521 ctgaccatgc tgcggtgcag cgtgtgggtc cttctgcgtc cgtgggcgag tcagctcgtg
     2581 gtccggtcgt cggccttagc gttgcggagt tgcgcttgac cgactcgatg cgcttgaccg
     2641 cgctggcctt gctctcgtag cgctcgctgg agctgcctcg ccgccgacga ccgggcaggc
     2701 tcccacgatg ctcggcgagg gggcggcttc ctcgccgttg ttggtcagct gcagccggcc
     2761 tatgggcgcc ggttggaccg gctgtggtct tggacgtcga tctgttcctt gcggacctcc
     2821 tcggttacct gctgctggtc ggtgacggtc tccttgtcca gccggacccg ttccttgggg
     2881 accacccgct tgtccaccac tggctgctcc tcgtggagca ccacctcatg cccagcgtcg
     2941 gtggccttgg ggtcgctggt cgcggcgtcg acgttggcgt cggctggcgg ctcgtgctcc
     3001 agtcggacct cctcgcgccg caccggcacg gtgacctgct gctcctcggt ggtcacgtac
     3061 ttgcgcagcc gcacccggcc ccgctcccgg gtttgcgtgc cgacccgcaa ctcctcctcc
     3121 gaccgagtca tcgcgtcgtc gcccatctgg ccagacgccg cctgcctggt gtcgcggccg
     3181 gggttgcttg ccggttcggt ggtgtcctgg ccggcctctc gggcactggc gggcagggcg
     3241 gcgtcggttg tgcctcccgg gccttggcgc ggcgtaccgg cgtggctggc gtcgtagtcc
     3301 aggccgtagt gacggaacag ctgccgctcc tcggccaccg acaggggccg gtccagcggg
     3361 gtgccgggcg cgtccttgac cagttgcctg tcgtagggca cccggacctc gctgccagag
     3421 acggtggcct gggcaatggg aacgaacgtc gatgtagtgc cggccgcgcc gaggctgacc
     3481 agcgcccact ccggctggcc tgaccgatca tcgacataga tggcgtcgat gcggccgatg
     3541 aggccgccgt cgcggtccat catggtatgg ccgcgccaac cgtggacggt cccgatatcg
     3601 gccatgacga tcgttcctcc tgcgatctgt gggagcgaga gccgggtccc gcacccatcc
     3661 tggcgtggac cttcgggatg aggcccagcc cctggcgtac ccgcccagac cacgtcgtac
     3721 cgcccacgca acaccgggaa acgcggctaa tcaacgggtg agcctgaccg gtccacgagc
     3781 gcggctgagg cttgctgcga ccaggacggc cgcccgtcgg tgcgcttcct tcgtcggccc
     3841 atccatggac ccctcagtcg gccagacggg ctcctacgtg gcgaagcggg gcgggaaccg
     3901 ccgactggtt ccggccgagg tttccacggt gtggcgggcc gcccaacgtg cgaccatgac
     3961 gccaggagcg gcctaccgca ggacgaggag cagaggagtt gagatcgtgg acggcgcgac
     4021 ggtgtccgtg gcggtcgttg ccagccgcac cgaggcggag ctgatcgtcg gcatgctacg
     4081 cactcatggc gtgagcgcga cggtgtcggc agacgatgcc ggaggacagt acccgcagat
     4141 gcagatggac ggtgttcggg tgctggtcgc ccccgccgat gaagcctcgg cccgtcggct
     4201 cctcgacacc gccggtgaca ccctcgagta ggccggtcag cccaagacca ttcggcgatg
     4261 gacgagccga cgcggcgtga cccgcgcctg ggcggcactg tcggacgccg attgacctat
     4321 gacagaccgg cttcggatga ccggtgatgg tcgtatccgg ctgccggccg gagcggtcgc
     4381 ggacggtcac cccc
//
LOCUS       DATADN010000776         4981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1459098, whole genome shotgun sequence.
ACCESSION   DATADN010000776 DATADN010000000
VERSION     DATADN010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459098"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VHH53_10105"
     CDS             <1..352
                     /locus_tag="VHH53_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2290520.1"
                     /translation="SVRGAFADGTVSSVSGTLSGPAQVQKLVEIDGRHFDLRAQGEAI
                     FMEYPDRPGVMGTVGTVLGSADVNIEAAQISQTTAGTDAVMMLRVDQAVPREVLDAIG
                     ASVGARTVRSVTFG"
     gene            502..4002
                     /locus_tag="VHH53_10110"
     CDS             502..4002
                     /locus_tag="VHH53_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2290521.1"
                     /translation="MLTVIAFAGFNGLASGQPDTVPLAVGLSPAAGLPPAPLQGKIGP
                     QLDATQGPVTVFVELAQTPVVNVFHAQRSAGRQATAAARVANAAKSQVERAADRALGM
                     LRHRTSGVHELFRTTNAVPGLAVIADADAIRQLAGSPDVRSVYRIVPKTAMNSSAVQL
                     TRTLRAWQQTGRLGDGVRIGIIDDGVDYTHADFGGPGTPAAYTAIDRTMIQPSYFPTR
                     KVVGGYDFAGDGYDGGAAGGPLGAIPRPDPNPLSCGQHGNHVAGTAAGFGVNADGSTF
                     RGDYQQLDPAALNALRIGPGTAPHALLYALKVFGCRGATALTAKAMDWALDPNGDGDF
                     SDRLDVVNLSLGSDYGAPDDPDSLFVRQLAVNGVLPVFAAGNGGDLYDIGGAPGNTPE
                     ALTVASTRDSAVLRDGAQVTAPSGVGDQIVGGQYSQDFPGYDGLDRTAGVVGLSDPAN
                     ADGCQPYSAADAAAMMGKHAWLEWDDNNATRRCGSKVRADHALAAKAAGVLLSSADEH
                     FAASILGNAGVPMFQLTGPDTAALRPSLQAGTLVVRLAGKLRTSVHTDDPRITDTVSE
                     FSSRDVRGPALKPDVAAPGDTIASALTGSGSDLLVISGTSMASPHVAGIAALVRQAHP
                     DWSVAEVKAAVANTAGADVHSLDGQQGPISAPNRVGTGRVDAAAAVRTQVLAMVPDDP
                     GQVSAGFGVVQATGPVTLRKTITLVNKGATAVRYAVAYQPVTTIPGVDYHLSSPSVDI
                     APRGVAALTVTLRIDDPSALRRTADPTIEKTQLGAARQFVADASGRIVLSAVDAVTLR
                     VPVYAAPRPVSDLYALESMHFQRDDRQAMLALFGRGVDQGIGDAAYRSLISVLQLQTE
                     SGRLVQCTGAFVAACTPNGTAQGGDIRYVGVTSTAALAVARGRPKDALLAFGITTWDD
                     WYNVGSNTIPFVNIDTTGDGLPDFEVRGTKLTGTDVLVASTTDLHAPPGSPPVQVLPV
                     NGQFGDVDSGVFDSNIIVLPVSLAALGIDPLAATAPMSYTTAVASHYASPVSVGEVID
                     STAPALFDAVRPGLWAQGTGDAALSYLARPGTALMINRDAIAARAQRAAQLLVLHPAN
                     PSGNRVQVVPVSAPGSALVGGPGPAVPLPVPAAPPVPVESPDPARAGFRTAESLYSAR
                     RLGLLRPFAYPSCGARRRCG"
     gene            4057..>4981
                     /locus_tag="VHH53_10115"
     CDS             4057..>4981
                     /locus_tag="VHH53_10115"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX2290522.1"
                     /translation="MRLAVIPGDGIGPEVIGEALKVLNEVAPDAETTPYDLGAARWHS
                     TGELLPESVLNELRQHDGILLGAVGDPSVPSGILERGLLLRLRFELDHHVNLRPARLY
                     PGVRSPIADPGSIDMIVVREGTEGPYAGNGGLLRKDTPHEIATEVSLNTAFGVERVVR
                     DAFARAAARPRRHLTLVHKTNVLSHAGSLWSRIVEEVSLQHPDVTVAYQHVDAATIHL
                     VTDPGRFDVIVTDNLFGDILTDLAAAVTGGIGLAASGNLDGTRRNPSMFEPVHGSAPD
                     IAGQGIADPTAAVLSIGLMLAHLGMDEPSRRV"
BASE COUNT          768 a         1678 c         1728 g          807 t
ORIGIN      
        1 cagcgtgcgg ggtgcgttcg ccgacggcac cgtgtcctcg gtctcgggaa cactgtctgg
       61 gcctgcgcag gtgcagaagt tggtcgagat cgatggccgg cacttcgacc ttcgggccca
      121 gggcgaggcc atcttcatgg agtacccgga ccgtcctggc gtgatgggaa ccgtcggcac
      181 ggtgctgggg tcggcggacg tcaacatcga agcggcccag atcagccaga cgactgcggg
      241 tactgatgcg gtcatgatgc tgcgggtcga tcaggccgtg ccacgggaag tgctggacgc
      301 catcggggca tcggttggcg cccgcaccgt ccgctcggtc acgttcggct gacctttcgc
      361 ggcaccgaac tacccatccg gactggtcac aaaatgcgat cgactggata cggtccgtca
      421 gtgcgtcgca tccgggtacg tcgctcgtgg gctcgccgcc cccgagtctt tccgaagagt
      481 cctgcgacgg tcgtggttgt cgtgctcacc gtgatcgcct tcgcgggatt caacggcctg
      541 gccagcggcc agcccgatac ggtgccgctt gcggttggct tgtcgcccgc ggccggcctg
      601 ccgccggcac cgctgcaagg caagatcggc ccacagctgg acgcgacgca ggggcccgtg
      661 acggtgtttg tcgagctcgc tcagaccccg gtggtgaacg tctttcacgc ccagcgcagc
      721 gcggggcggc aggccacggc cgcggcgcgg gtggccaatg ccgccaagag ccaggtggag
      781 cgggccgccg accgggcgct tgggatgctg cgtcaccgca cttcgggcgt tcacgagctg
      841 ttccggacca ccaacgcggt gcccggattg gccgtgatcg ccgacgccga tgcgatccgt
      901 caactggccg gctcgcccga cgtccgctcg gtgtacagga tcgtgcccaa gaccgcgatg
      961 aactccagcg ccgtgcagct gaccaggaca ctgcgcgctt ggcagcagac cgggcggctg
     1021 ggggatgggg tacggatcgg catcatcgac gacggggtcg actacacgca cgcggacttc
     1081 ggcggtcccg gtaccccggc ggcctacacc gccattgacc gcaccatgat ccagccgagc
     1141 tacttcccga cccgcaaggt agtgggtgga tacgacttcg ccggtgacgg ctacgatggc
     1201 ggcgcggccg gtggaccgct gggggccatc ccacggccag atcccaaccc gctgtcctgc
     1261 ggccaacacg gcaaccacgt cgccggcacg gcggccggat tcggcgtcaa cgctgacggc
     1321 agcactttcc gcggtgatta ccagcagctg gacccggcgg cgctcaacgc cctgcggatc
     1381 ggccccggca ccgccccgca cgccttgctg tacgcgctga aggtcttcgg gtgccgcggc
     1441 gcaacagcgt tgaccgccaa ggcgatggac tgggcgctgg accccaacgg cgatggcgac
     1501 ttttccgacc ggctcgacgt ggtgaacctg tcgctgggct cggattacgg tgctcccgac
     1561 gacccggaca gcttgttcgt ccgccaactg gcggtcaacg gggtgctacc ggtcttcgcg
     1621 gcgggcaacg gcggtgacct ctatgacatc ggtggagcac cgggcaacac tcccgaggcg
     1681 ctcaccgtgg ccagtacccg ggactccgct gtgctgcgcg acggggctca ggtgaccgcg
     1741 cccagcggcg taggtgacca gattgtgggt ggccagtaca gccaggactt ccccggctac
     1801 gacggcctgg atcgcaccgc tggggtggta gggctgtccg atcccgccaa cgccgacggc
     1861 tgccagccct attcggcagc cgacgctgcc gcgatgatgg gcaagcacgc ctggctggag
     1921 tgggacgaca acaacgccac ccggcgctgc ggttcgaagg tgcgggccga ccacgcgctg
     1981 gccgccaagg ccgcaggagt gctgctgtcc tcggcggacg agcatttcgc cgcgagcatc
     2041 ttgggtaatg caggggtgcc gatgttccag ttgaccggtc ctgacacggc ggcgctgcgc
     2101 ccgtccctgc aggctgggac gctcgtggtg cggctcgcgg ggaagctgcg gacctcggtg
     2161 cacaccgatg atccacgaat caccgacacc gtcagtgagt tcagctcacg tgacgtgcga
     2221 ggtcccgccc tcaaacccga cgtggcggca cccggggaca ccatcgcgtc ggcgctaacc
     2281 ggcagcggat cggacctcct ggtgatcagc gggacgtcga tggcgtcccc acacgtcgcg
     2341 ggcatcgcgg cactggttcg ccaggcgcat ccggactgga gtgtggccga ggtcaaggcc
     2401 gcggtggcga ataccgcagg agcggatgtc cattcgctcg atggtcagca gggcccgatt
     2461 tcggccccca accgggtcgg caccggccgg gtggacgctg cagccgcggt gcgtactcag
     2521 gtgctggcga tggtgcccga cgaccccggt caggtcagcg ccggcttcgg tgtggtgcag
     2581 gcaaccggtc cagtcaccct cagaaagacg atcacactgg tgaacaaggg tgcgaccgcg
     2641 gtccggtacg cggtcgccta ccaacccgtc accaccattc ccggagtcga ctaccacttg
     2701 tcgagtccat cggtcgacat cgccccgcgc ggggtcgcgg cactgactgt cacgttacgc
     2761 atcgacgatc cgtcggcact gcgccggacc gccgatccga cgatcgagaa gacccaactc
     2821 ggtgcggccc gccagttcgt cgccgacgcg tctggccgga tcgtgctcag tgcagtggat
     2881 gcggtaaccc tgcgggtgcc ggtctatgcc gctccgaggc ctgtgtccga cctgtacgca
     2941 ctcgagagta tgcactttca gcgcgatgac cggcaggcca tgctggccct gttcggccgc
     3001 ggagtggacc agggcatcgg agatgccgcc taccggtcgc tgatcagcgt cctgcagctg
     3061 cagactgaga gtggacggtt ggtccagtgc acgggtgcat tcgtggcagc gtgcacgccg
     3121 aacgggaccg ctcagggagg tgatattcgc tatgttggtg tgacgtcgac tgcggcgctg
     3181 gcggtggccc gaggcaggcc gaaagacgcg ttgctcgcat tcgggatcac cacctgggat
     3241 gactggtaca acgtcggcag caacaccatc ccgttcgtga acatcgacac caccggggat
     3301 gggttgcccg acttcgaggt ccgcgggacg aagctgaccg gaaccgacgt gctcgtcgcg
     3361 tcgaccaccg acctgcatgc gccgccagga tccccgccgg ttcaggtgct gccggtcaat
     3421 ggtcagttcg gggacgtgga ctcgggcgtc ttcgacagca acatcatcgt gctgccggtg
     3481 tcactggccg cgctgggaat cgacccgttg gcggcaacgg cgccgatgag ctacaccacg
     3541 gctgtcgcaa gccattacgc cagtccggtg agcgtcggcg aggtcatcga cagcacggcc
     3601 ccggcgttgt tcgacgccgt ccggccggga ctgtgggccc aggggaccgg ggatgccgcg
     3661 ttgagctatc ttgcccgccc agggaccgcg ttgatgatca accgtgacgc catcgccgcg
     3721 agggctcaac gtgcggccca gctgctggtg ttgcacccgg ccaaccccag tgggaaccgg
     3781 gtgcaggtag tgccggtgtc ggcgcctggc tctgcgttgg tgggcggacc aggtcctgcc
     3841 gtgccgcttc cggtgcctgc agcgcctccc gtcccggtgg agtcccctga ccccgcgcgg
     3901 gccgggtttc gcacggccga gtcattatat tcggcacggc ggctgggcct gcttagacca
     3961 ttcgcgtatc catcgtgcgg tgctcgacgt cgctgcgggt aacctgcccg cggcagaggg
     4021 ctccacggca gggggcccgg gcattggagg tggttcatgc ggctcgcggt gatccctggc
     4081 gacgggatcg gtccggaggt gatcggtgag gcgctcaaag tcctgaacga ggtcgctccc
     4141 gacgccgaga ccacgcccta cgatctcggt gccgcgcgct ggcactccac cggggaacta
     4201 cttcctgagt cggtgctcaa cgagctacgc cagcacgacg gcattctgct cggcgcagtg
     4261 ggggacccct cagtgccaag cggaatcctt gagcgtgggc tgctgctgcg gctgcgtttc
     4321 gaactggacc accacgtgaa cctgcggccg gcccggctct accccggggt gcgcagcccc
     4381 atcgcggacc ccggcagcat cgacatgatc gtcgttcgcg agggcaccga aggcccgtat
     4441 gccggcaacg gcgggctgct gcgcaaggac acgccgcacg agatcgccac cgaggtcagc
     4501 ctgaacacgg ccttcggcgt ggagcgagtc gtgcgtgacg cgttcgcccg tgctgcggcg
     4561 cgcccgcgca ggcacctgac actggtgcac aagacgaacg tgctgtcgca cgctggatcg
     4621 ctgtggtcgc gcatcgtgga ggaggtgtcg ctgcagcatc ccgacgtgac ggtggcctac
     4681 cagcacgtcg atgccgccac catccacctg gtcaccgacc cgggccggtt cgacgtgatc
     4741 gtcaccgaca acctgttcgg tgacatcctc accgacctcg cggcggctgt gaccggtggg
     4801 atcgggctgg ccgccagcgg gaacctggac gggactcgcc gcaaccccag catgttcgag
     4861 ccggtgcacg gctccgcccc ggacatcgct ggtcaaggca tcgctgaccc gaccgcggcg
     4921 gtgttgtcga tcggcctgat gctcgctcat ctcgggatgg acgaaccgtc tcgccgggtc
     4981 g
//
LOCUS       DATADN010000777         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1009110, whole genome shotgun sequence.
ACCESSION   DATADN010000777 DATADN010000000
VERSION     DATADN010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009110"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1340)
                     /locus_tag="VHH53_10120"
     CDS             complement(282..1340)
                     /locus_tag="VHH53_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX2290523.1"
                     /translation="MIFVAGLGLVLAALVAWDAAVTVLHPDAEGLLAKSIRRAMWRLT
                     ALVSMCLPRVDRHVLGLAGPVIVAMTFVGWIVLITLGITLVVWPMLGDDFEVQANLGS
                     LTFLDALYFASGTVTVLGYGDITPVSTGGQLISVFGAAIGFTMFTGMATYAIEIVSGL
                     ATRNRFTLAVHDDTRGGGGATMLAECLAEAGADETRERCRSWAEYLRAVDDMVHRYPL
                     VAFTYRSHRDEYDPEPALRHVAEATVTALVAAGREPALRATAEALCSALTRLQCTIAD
                     KYLGSDIARRLADPQPTAQDRQAVANVASLLSTHLGCSVPAAEHQVATEAVHRCRTFL
                     AGLHHWSRSSMPPHEWDD"
BASE COUNT          290 a          500 c          527 g          252 t
ORIGIN      
        1 cacaccggag ccatacgatg aattgcgcat tcgaagctgt caaaagatgt gcacggcgcc
       61 ttgggcatga accgggtaga tcgacgtggc ctctcgccga gctgaccgcg cgcagaggca
      121 ttacccctct ccggcaggga agtaagaaca gtgaccgggg agggttgagt cgacggtgcg
      181 ctacggcccg cgccccggtg cgggagacgc tgagtgcggt ggaccagttc cgcgggctcc
      241 aggggcaggc actgaatctt ctcggactgg tcctcggcag ctcagtcgtc ccattcgtgc
      301 gggggcatac tgctgcgtga ccagtggtgc agcccggcga gaaaggttcg gcaacggtgc
      361 actgcctcag tcgctacctg gtgttcggcc gcggggacgg aacaaccgag gtgggtcgat
      421 agcaagctgg caacgttggc caccgcctgg cggtcctgtg cggtgggttg ggggtcggca
      481 agccgccggg cgatgtcgct gccgaggtat ttgtccgcga tcgtgcactg gaggcgggtc
      541 agcgccgagc agagtgcctc ggccgtcgcg cgcagtgctg gctcgcgtcc agccgcgacg
      601 agtgcggtca ccgtggcctc ggcgacatgc cgcagcgcgg gctccggatc gtactcgtcg
      661 cggtgtgagc ggtaggtgaa tgccaccagc gggtatcggt gcaccatgtc gtcgaccgcg
      721 cgcaggtact ctgcccaact tcggcagcgc tcgcgagtct cgtcggcgcc tgcctcggcc
      781 aagcactcgg cgagcatggt ggcgccaccc ccgccgcggg tgtcgtcgtg gacggccagc
      841 gtgaaccggt tgcgggtggc gaggccgctg acgatctcga tcgcgtaggt tgccatcccg
      901 gtgaacatgg tgaacccgat ggcggccccg aacaccgaga ttagctggcc accggtggac
      961 accggcgtta tatcgccgta gccgagcacc gtgaccgtgc cgcttgcgaa gtagagggcg
     1021 tcgaggaagg tgagcgagcc gagattggct tggacctcga agtcatcgcc cagcattggc
     1081 cagaccacca gtgtgatgcc gagggtgatc agcacgatcc agccaacgaa ggtcatcgcc
     1141 acgatcaccg gcccagccaa cccgagtacg tgcctgtcga cccgcggcaa gcacatactc
     1201 accagcgccg tcaatcgcca catcgcgcgg cggatgctct tggccagcag cccttcggcg
     1261 tctggatgca gcacggtaac cgcagcatcc caggccacca acgcggctag cacgagtcct
     1321 aaacccgcca caaagatcat acagacgtgt acccactgcc gccgggagct gatcccatac
     1381 cgcacctcac ggctcgaccc gctgtggcgc aaccggcttc gataactcgg tcacccggcg
     1441 accctgctcg ccgcacaagg tcccgatgga aagtttgtag agaatccagg acgcacacga
     1501 tctggcgtgg aagtggcaaa cggcagtgtg cctcctgagg tctacaacgg accgagatga
     1561 aatgatgac
//
LOCUS       DATADN010000778         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_237547, whole genome shotgun sequence.
ACCESSION   DATADN010000778 DATADN010000000
VERSION     DATADN010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237547"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VHH53_10125"
     CDS             complement(<1..545)
                     /locus_tag="VHH53_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter"
                     /protein_id="HEX2290524.1"
                     /translation="MTANIPGLEALVDVASTPSETEERGTRRTHVSRRRLYLRRFLRN
                     RGAVVGLAVLALLVLFALFGSLLTTYTYTDVDFASLSSAPSPEHLFGTNGAGNDTYAQ
                     AVHGLQRSLVIALVVSALTTMIAAFVGAAAAYLGGLAEKIILAVIHFLLVVPSFLILA
                     LISQRAHGDWSVLILVLTVFG"
     gene            complement(542..1531)
                     /locus_tag="VHH53_10130"
     CDS             complement(542..1531)
                     /locus_tag="VHH53_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2290525.1"
                     /translation="MLRYVARRVVSWVLMIVVATNLTYFLANWFLDPRANYLQLRPPR
                     SPEQIDRALAQYNLSPDTPLLVRWWHWLSNIVLHWNWGFSPEGEAVNGQIAFRIGVSA
                     QLVLLSTILSTVIGIALAVYTAARQYRLADRLTQTLSIITLNIPVPVASLVVVLLAIA
                     LNRSLDETLFFVAGSQSPTVTGFWSLVLDRLRHLALPTIALTVISYAGYHLLQRSLLL
                     DNINADYVRMARAKGLTRSEAIRRHGLRTSLIPVATSIAFTVPALFTGAVLTETIFGW
                     EGMGRYFVTTISKNDVHGAVAVAAFGALMTAVGAILADLAVVALDPRVRVSGS"
BASE COUNT          382 a          635 c          646 g          273 t
ORIGIN      
        1 cagccgaaca cggtcaacac gaggatcagc acggaccagt caccgtgggc ccgctgcgag
       61 atgagcgcca ggatcaggaa cgacggcacc accagcagga agtggatgac cgcgaggatg
      121 atcttttcgg cgagaccacc aaggtacgcc gccgctgcgc ccacgaacgc cgcgatcatc
      181 gtcgtcagtg ccgacacaac cagcgcgatg accagcgacc gctgcaggcc gtgcacggcc
      241 tgggcgtagg tgtcgttacc ggcgccgttg gtgccgaaca gatgctcggg cgagggcgcc
      301 gagctcaaac tggcgaagtc gacgtcggtg taggtgtagg tggtgagcag cgagccgaac
      361 agagcgaaca ggaccagcag ggccaagacg gccaacccga ccaccgcgcc ccggttccgc
      421 agaaaccgac gcaggtagag ccgtcgccgg ctgacgtgcg ttcggcgggt gccccgttcc
      481 tcggtctcgc tcggcgtcga cgccacgtcg acaagggcct cgaggccggg gatgttggcg
      541 gtcacgagcc gctcacccgc acgcggggat cgagggccac cacggccagg tcggccagga
      601 tagcgccgac cgcggtcatc agggcgccga acgcggcgac ggctaccgcg ccgtgcacgt
      661 cgttcttgct gatcgtcgtc acgaagtagc ggcccatgcc ttcccaaccg aagatcgttt
      721 cggtcaacac cgcgccggtg aacagggcgg ggacggtaaa cgcgatggag gtggcgaccg
      781 gaatcagcga ggtccgcagc ccgtgccgac ggatcgcctc ggaccgagtc agccccttgg
      841 cccgcgccat ccgcacgtag tcggcgttga tgttgtccag cagtagcgag cgctgcaaaa
      901 gatggtagcc ggcgtaactg atcactgtca gggcaatggt cggcagggct agatgccgca
      961 gccgatccag caccagcgac cagaacccgg tgaccgtggg ggattgggag cccgccacaa
     1021 agaacagcgt ttcgtccagg gatcggttga gcgcgatcgc cagcaacacc accaccaggc
     1081 tcgccaccgg aaccgggatg ttcaacgtga tgatcgacag ggtctgggtg agccggtcgg
     1141 ccaagcggta ctgacgggcg gcggtgtaga cggccagcgc aatgccgatg accgtggaca
     1201 ggatcgtcga cagcagcaca agttgggcac tgaccccgat ccggaacgcg atctggccgt
     1261 taacggcctc tccctcggga gagaatcccc aattccaatg cagcacaatg ttgctgagcc
     1321 aatgccacca gcgcacgagc agcggggtgt cggggctcaa gttgtactgc gcgagagcgc
     1381 ggtcgatctg ctccggcgat cgaggcgggc gcagctgcag gtagttcgcc cgcgggtcga
     1441 ggaaccagtt cgccaggaaa taggtcaagt tggtagccac cacaatcatg agcacccagc
     1501 tgacgactct ccgggccacg tacctcagca cggcggcatc gcccgacatc caccagaggc
     1561 tgccctgcgg cacgagccgc cgacgaacct gaccgccacg cgcccgatcg tcctcgtcgc
     1621 ccggtcaccg tcaagaccat ggcgacgaga tcgtgcagcg tgccctgtct gtagacctgc
     1681 gacaggggcc gccccggaag cgctcctgac gtgctcgcac ccgaagccac ccggcggttg
     1741 gctcctgccc atgtggaccc gataaggcac ctccggaagc gcatggccgc gctggcttcg
     1801 ctctgaaaga gccatcgtgc accggagctg ataccgcgtt ccgattgccg aaacccgttc
     1861 tccgcctggg tgtgccgacc gagtgaccac gcagggccga ggcaaagtca gatcgtgggc
     1921 tctgacctgg ggtgat
//
LOCUS       DATADN010000779         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_839208, whole genome shotgun sequence.
ACCESSION   DATADN010000779 DATADN010000000
VERSION     DATADN010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839208"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1296)
                     /locus_tag="VHH53_10135"
     CDS             complement(187..1296)
                     /locus_tag="VHH53_10135"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2290526.1"
                     /translation="MPRPGDRVLGYLVTGMDVTSIMSSRKLFYWPAVGTGMVFVALLA
                     ASLLIGGVANSAPPGGPGHPTEALAPTATTTALAVSPVSPVAQGTRVTLTATITPSTA
                     AGTVQFKDATTDLGTPVPVSNGTASGSTSMLALGSHSLTAVFTPATPATFSPSTSPAV
                     TYVITGAPGTATNAATANNAGMAGGAATATNTATATNTVLTTSPASTVTQGTPVILTA
                     TITPSTAVGTVQFIDATTNIGDPVPVSTNGTASGSTSMLAPGPHQLTAVFTPANPAMF
                     SPSTSPAIPLTVTGSATGTPHTVTQQSGLSLDIQVTIHGDPDNHVVVLDDCGCSSGRG
                     ISIPILDGRVTTLDGRPAVLNGNGPLNGIVSVLLD"
     gene            complement(2337..>2597)
                     /locus_tag="VHH53_10140"
     CDS             complement(2337..>2597)
                     /locus_tag="VHH53_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290527.1"
                     /translation="EFGRFANDARDHHAEELAGWPSEHPVMVPAGFPPRCLSVLLHDP
                     AAVALFLCAHMTADDLMELILILADAAADVLRARRAIDDACG"
BASE COUNT          460 a          773 c          912 g          452 t
ORIGIN      
        1 ccgcgacgcc ctcgtcacgc ttgacaccaa acaacttgac agcgcctcag ccgattggga
       61 caccaggatg gcagccctga cctcgggcag gggtcaccga ggcggtgccg tcggtgaccc
      121 ctcccggcat cctgtgtcgg cgagcgtttg aagtggtggc gtcggttgtc ggtggttggg
      181 gttagctcag tcgagtagga cggagacaat accgttgagt ggtccgttgc cgttgagaac
      241 agccggtcgt ccatcaaggg tagttacccg gccgtcgagg atcggtatcg agatcccgcg
      301 gcctgatgag catccgcagt catcgagaac aaccacgtgg ttgtctggat caccatgaat
      361 ggtcacctga atgtccaggg ataggccaga ttgctgggtg actgtgtgcg gcgttccggt
      421 tgccgatccg gtcaccgtta gtggtattgc tggtgatgtt gaggggctga acatggcggg
      481 gttggcgggg gtgaataccg cggtcaactg gtgtggtccg ggtgccaaca tcgacgtgga
      541 ccctgacgcg gtgccgttgg tgctgaccgg caccggatcg ccgatgttgg tggtggcgtc
      601 tatgaattgc acggtgccca ccgcggtcga cggtgtgatc gttgcggtca gtatcaccgg
      661 ggtgccttgg gtgaccgtgg aagcggggga tgtcgtcagc acggtgttgg ttgcggtcgc
      721 ggtgttggtc gccgtggcgg cgccgcccgc catgccggcg ttgttcgctg tcgcggcgtt
      781 ggtggcggtc ccgggggcac cggtgatcac ataggtcacc gcaggtgatg tcgaggggct
      841 aaaggtcgcc ggggtggccg gagtgaacac cgcagtcagt gagtgtgacc ccagggccaa
      901 catcgaggtt gatcccgacg cggtgccgtt gctgactggt accggagtgc cgaggtcggt
      961 ggtggcgtct ttgaattgca cggtgccggc cgcagtcgat ggagtgatcg tggccgtcag
     1021 ggtcacccgg gtaccctggg caaccggaga aaccggggat acggccagcg cggtagtggt
     1081 ggctgtcggg gcgagggctt cagtcgggtg accagggcct cccggtggcg cggaattggc
     1141 cactccaccg ataagcaacg acgctgccag gagcgcgaca aagaccatac cggtccccac
     1201 cgcaggccag tagaagagtt ttctgctcga cataatggac gtcacatcca ttccagtcac
     1261 aaggtagcca aggactcggt cacccggcct tggcatatcg gcgttgagcg cgtccgagat
     1321 cagatcgtca cgatgaaaag gctgcgccgc gtgtgcaagc agcgacacgt tgagccgcgc
     1381 gacgacgatt gccccctcgc caacacaccc ctcagtgcag taactatgat ggggtgtcca
     1441 gccgcgcctg gtgtcactca caccggtcga gaccgcaccc ggtcgggtaa ttttgacgct
     1501 gagtcacgtg ccggtgagtc ggtgtggtcg gccgctggcg tcccgacggc cccgacaagg
     1561 actccagcat ccggggcagt gctggagcct gctcgtcact gcgacgcagc cggtgatcag
     1621 gcgtgtgaag cctgaataac ggtgtcgtga gcccatcctg gggcacaggg tggctggggt
     1681 gccgcgctca tggcgccagt ccaattcacc gctgtccccg gcggcgctca tccagattca
     1741 ccaccgtcgg cggtggtcga ccatcagaag gagcagccga gggacgtgac ggcgcgggcc
     1801 gagagctggc ccgcggtggg cgcaccctgg ccgctggcaa cttggtggtg ttcgcagcag
     1861 aaccacccgg tgcaggggca atgggcaccg caggggtcac cggcggggcc tctggtgtcg
     1921 ggaaaacagg cacgggtacc gaagaggtcg tcactggggg caccgagaca accggtgcgg
     1981 ggggcaccga gacaaccggt gcgggcaaca ctggcacgaa gccttgcccg gtagaccgcg
     2041 ggtccgggcg ggtgtgcgtg gtgatgacca atacaccgac catgccccca gcaagaacaa
     2101 gacaccccac ggccgcaagc cgtctcaacc ggcgctgccg gtgtatcgcc tgatgccgca
     2161 aggcctccca gacttcccag acttcggcgc gagcctctaa cgggatcacc gccggcgaag
     2221 gtggcacctc actgggcgct gcgctgcgcc tctgcctggc agcggtcgct tctgccccca
     2281 tccggtcccg cagagcacgc agctcctcaa ccgtcaggat ctggctggca tcagactcat
     2341 ccacaagcgt cgtcgatggc gcgtcgggct cgtaatacat cggctgccgc atcggcgagg
     2401 atcaggatca gctccatgag atcatccgct gtcatatggg cgcacagaaa tagtgcgaca
     2461 gcagcaggat catggaggag aacggacagg cagcgcggtg gaaaaccggc cgggaccata
     2521 accggatgct cggacggcca tcctgcgagc tcctcggcgt gatgatcgcg ggcgtcgttg
     2581 gcgaacctgc cgaactc
//
LOCUS       DATADN010000780         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_839311, whole genome shotgun sequence.
ACCESSION   DATADN010000780 DATADN010000000
VERSION     DATADN010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839311"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VHH53_10145"
     CDS             complement(<1..467)
                     /locus_tag="VHH53_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2290528.1"
                     /translation="MRIVITGATGNVGTSVIESLASDTGITSILGLARRLPNWQAPKT
                     QWAQADVAKHELASHLRGADVLIHLAWLFEPTHNPVTTWRANVEGSIRVFRAAADTGV
                     RTVVYASSVGAYSPGPKDRRVDESWPTHGWPNAGYTREKAYVERVLDTFEHEHP"
     gene            complement(523..855)
                     /locus_tag="VHH53_10150"
     CDS             complement(523..855)
                     /locus_tag="VHH53_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290529.1"
                     /translation="MGRPEGVRRPGGEVHAWARGTNQTLCGRALSRSWLGRYPHVDWE
                     DIRPESGRHADAVRRVCPRWPGPARGENRRSGAAEAPATTSELSRAVAVDEWCTIRLA
                     LDPDRADA"
     gene            complement(1123..1947)
                     /locus_tag="VHH53_10155"
     CDS             complement(1123..1947)
                     /locus_tag="VHH53_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290530.1"
                     /translation="MHRLSRRAFLFTTVGTATAIIDGCTWATSPKGTPDPLNGFAQPG
                     VDAQGGPARLTSRLPDRNLLKSESKPGLRALQLGPTGRQVLLHVPPGLDEGPPAVLVL
                     TLHGAGGNAPAGLAPLRPLADANRLLLLAPTAQGSTWDAVTGSWGPDIGRIDQALAEV
                     FTSYRVDSRRVAVSGFSDGASYALSMGLANGDLFTHIIAFSPGFVVPGPRVGTPAIYI
                     SHGRDDAVLPIERTTRRISPRLRKSGYPVVVHEFEGSHTVPPAITEDAVRWVLGGP"
BASE COUNT          344 a          709 c          752 g          362 t
ORIGIN      
        1 ggatgctcgt gttcgaacgt atcaaggacc cgctcgacgt aggccttctc gcgggtgtat
       61 ccggcgttgg gccagccgtg ggtaggccaa ctctcatcga ctcgtcggtc cttggggccc
      121 ggtgagtatg cgcccaccga ggaggcgtac acgaccgtcc gcacgcctgt gtccgccgcc
      181 gcgcggaaga cccggatgct gccctcgacg tttgctctcc aggtggtgac cggattatgg
      241 gtgggctcga acaaccacgc cagatggatc agcacgtcgg cgccgcgcag gtgcgaggct
      301 agctcgtgtt tcgcgacgtc cgcctgcgcc cactgggtct tgggcgcctg ccagttcggc
      361 agccgccggg ccaggccaag tatcgaggtg atgcctgtat ccgaggcgag agactcgata
      421 acgctcgtgc cgacgttgcc ggtggcgccc gtgatcacta tgcgcattct gtgaaggttc
      481 cccaaagggg aacaatcaaa cggcgctgtg tgaaggcaac gactacgcgt cggcgcgatc
      541 cggatccagg gcgaggcgga tcgtgcacca ctcgtcgacg gcgacggccc tgctcaactc
      601 cgacgtggtc gccggtgctt cggccgcgcc gctgcggcgg ttctcgccgc gggcgggccc
      661 cggccagcgc gggcacaccc gcctgactgc gtcggcgtgt ctgccggact ctggccggat
      721 gtcttcccag tcgacgtgcg gataccgccc cagccaagac cgactgagcg cccgcccgca
      781 aagagtctgg ttggtgccgc gtgcccaggc gtgcacctca ccgccgggcc ggcgcactcc
      841 ttcgggtcga cccactcgct cgatgccgcg accgcgtacc tcatcccgtc actctgcacg
      901 atcggacctt tgcttggccg ggacatcaga gtcggtgagc cgcctccgcg ttggacgaac
      961 gtggtccggg cagcaggtag ctcacggcca agcggggtcc caagccgctg ctgggaccgc
     1021 cggtggccat cgtggatcgg acatgcgacg cgatccgcat ggtcatcggc gctgatatgc
     1081 acattggcag cgggcggtga ggcatcgctg ctggcggaac tgtcatggcc cacccaatac
     1141 ccaccgcacg gcgtcttccg tgatggcagg cggcaccgta tgtgatccct cgaattcgtg
     1201 cactacgacg gggtaacccg atttgcgcag ccgcggagag atgcgccggg tggtgcgctc
     1261 gatcggcagc accgcatcgt cacgtccgtg ggatatgtat atggccggcg tgccgactcg
     1321 cggtcccggt acgacgaacc cgggtgagaa cgcgatgatg tgggtgaaca gatcgccgtt
     1381 ggccaacccc atggacaggg cgtaggaggc gccgtctgag aagccgctga cggcgacacg
     1441 cctggagtcg acgcggtagg aggtgaacac ctcagccaac gcctgatcga tgcgcccgat
     1501 gtccgggccc cagctaccag tgacagcatc ccaagtcgag ccttgtgcag tgggggctag
     1561 cagcagcagc cggttggcgt cagcaagcgg tcgcagtggt gccaggccgg cgggggcatt
     1621 cccgccagcg ccgtgcaggg tgagcaccag caccgcagga gggccttcgt cgaggccagg
     1681 cggcacgtgc agcagcactt gacggccagt tggcccgagt tgtagcgcgc gcaggcccgg
     1741 cttcgattca gattttagga ggttgcggtc ggggagtcgg ctagtcagcc gggctggacc
     1801 gccctgcgcg tcgacgcccg gttgggcaaa gccgttcaac ggatccggtg ttcctttcgg
     1861 ggaagtcgcc cacgtacagc cgtcaatgat cgccgtggcc gtccccacgg tggtgaacag
     1921 gaaagcccgt cggcttagtc gatgcatgac tcaagccatt gcccgcctga gcgtgctcgc
     1981 gcttgatgga cggaacttcc tcgtggtgta agggcccgtc gcaggccagc gtggagcatc
     2041 agcccggccg cggtggccag ggcaccagcc actgcgggtg tcggctcccg cggcccagcc
     2101 aatcgggcag tccgccggag tgccatcgtg ttggtcgctg ctccgtccgg cgcgcacaca
     2161 taggggg
//
LOCUS       DATADN010000781         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1290643, whole genome shotgun sequence.
ACCESSION   DATADN010000781 DATADN010000000
VERSION     DATADN010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290643"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VHH53_10160"
     CDS             <1..790
                     /locus_tag="VHH53_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2290531.1"
                     /translation="YHALIAPFMLPYLAGRPVNSHRYPDGVDRPGFWHKEVPTHAPQW
                     LTRWRNEEADPGETQWYAVIDSVPALVWLANFAAVELHPWTSRLPDVHQPTWALIDID
                     PGPSSTFDDVLVLARLYRVALDHLDVAAMPKVTGKRGIQIWVPVRSGYTFSDTRAWVE
                     KISRAVGHVVPDLVSWEWQKDRREGLARLDYTQNAINKTLVAPFSARPAPGAPVSVPI
                     QWEELDDPDLRPDRWTARTVIDRVHTCGDPLAPLVGRQQELPKV"
     gene            854..1813
                     /gene="ligD"
                     /locus_tag="VHH53_10165"
     CDS             854..1813
                     /gene="ligD"
                     /locus_tag="VHH53_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX2290532.1"
                     /translation="MMASLGAAPGGAGWAFEWKWDGVRAIVAAIPGLVRVTSRNGRDI
                     TAGYPELEVLAELVDRPVLLDGELVTLDQRGRPDFGLLQSRMHVRHPSPALMRSAPVQ
                     FYIFDLLHLDGPLLAKPYVQRRELLDQLQLTGGPVDIPAHYTGIGASVLLDLAGQHGL
                     EGVVAKRLDSRYEPGRRARTWIKTPLRHTQEVIIGGWRPGEGNRVGTIGALLLGVYDE
                     AGQLRYAGDVGTGFTDRMLRDLHAMLNSHARCTSPFDEVVPREHARGAHWIEPDLVGE
                     VEYRQWTADGRLRHASWRGLRPDRSPREIVLPKPRRSRWPPHR"
     gene            1893..>2619
                     /locus_tag="VHH53_10170"
     CDS             1893..>2619
                     /locus_tag="VHH53_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2290533.1"
                     /translation="MNRASAAESRAGVELTNLDHPLFDGADATKRDLVDYLDAVRDRI
                     LCALRDRPLSVIRVRPGQQPFMQKNVPKYAPDWVQTTTVWAEASRREVAYALCNDRRT
                     LLWFANQRAVEYHPTLVPDGASHPTHLVLDLDPPAGDAFPHVVRAAQLVRQALADAGL
                     AGAVKTSGSKGLHVFVPLDDRVGIEEAAAATRALAARAERLAPDVATTAFIREDRDGK
                     VFLDSTRSSGATVVAAYSPRARPG"
BASE COUNT          417 a          893 c          889 g          420 t
ORIGIN      
        1 ctaccacgcc ttgatcgcgc ctttcatgct gccctacctg gctggccgtc cggtgaactc
       61 gcaccgatac cccgatggcg tcgaccgtcc cggtttctgg cacaaggagg ttcccaccca
      121 cgcgccgcag tggctgaccc gctggcgcaa cgaggaagcc gacccgggcg aaacccagtg
      181 gtatgccgtc atcgacagcg tgcccgctct ggtctggctg gccaacttcg ccgcggtgga
      241 gttgcatccg tggacgtccc ggctgcccga cgtgcaccag ccgacatggg ccctgatcga
      301 catcgacccg ggtccgagca gcaccttcga cgacgtcttg gtgctcgcgc gcctgtatcg
      361 ggtggcgctg gaccacctcg acgtggcagc aatgccgaag gtcaccggca agcggggcat
      421 ccagatctgg gtacctgtca ggtccggcta caccttcagc gacaccagag catgggtcga
      481 gaaaatttcg cgcgccgtcg gccatgtggt gcccgatctg gtcagctggg agtggcagaa
      541 agaccgccgg gaggggttgg ctcgcctgga ttacacccaa aacgccatca acaagacgct
      601 ggtggctccg ttcagcgctc ggcccgcccc tggagcgccg gtgtctgtgc ccatccagtg
      661 ggaagaactc gacgatccag acctccggcc ggaccgctgg accgcccgga cggtgatcga
      721 tcgagtccac acctgcggcg atcccctcgc cccactcgtc ggccggcaac aagaactgcc
      781 gaaggtatag ctcggcacgc cgaacaccga caatgtggta gtggctgtct ccttgcctga
      841 tccggtggcg ccgatgatgg ctagcctcgg tgcggcgccc ggcggtgccg gttgggcgtt
      901 cgagtggaag tgggacggtg tccgggcgat cgtcgcggcg attcctggat tggtgcgtgt
      961 cacctcgcgc aacggccggg acatcaccgc cggctacccc gagctcgagg tgctggccga
     1021 gttggtggac cggccggtgc tgctggacgg cgaactggtc accctcgatc agcgcggccg
     1081 cccggacttc gggctgctgc agtcccggat gcatgtgcgg cacccatccc cggctctgat
     1141 gcgctcggcg ccggtgcagt tctacatctt cgatctgttg catctcgacg ggccgctgct
     1201 ggccaagccc tacgtacagc ggcgagaatt gctggatcag ctgcagctca ccggcgggcc
     1261 agtggacatt ccggctcatt acaccggcat cggggcctca gtgttgctgg acttggccgg
     1321 ccagcacggg ttggaaggcg tcgtcgccaa aaggttggac tctcgctacg aaccgggccg
     1381 cagagcccgt acctggatca agaccccgct gcgccacacc caagaagtga tcatcggcgg
     1441 gtggcgcccc ggtgaaggca accgcgtcgg caccatcggc gcgctgctac tgggggtgta
     1501 cgacgaagct ggacaactgc gctacgccgg cgacgtcggc accggcttca ccgaccgcat
     1561 gctgcgagat ctgcacgcca tgctgaacag ccacgcgcgc tgcaccagcc ctttcgatga
     1621 ggtggtaccc cgcgagcacg cccgtggcgc gcactggatc gagccggacc tcgttggcga
     1681 ggtcgaatac cgccaatgga ccgctgacgg ccggctacgg cacgcctcat ggcgcggcct
     1741 acggccagac cgcagtcccc gagagatcgt gctgcccaag ccgcgccgtt cccgctggcc
     1801 gccgcaccgc tgaggtggta ggaggggatt cgacgatcct cgggcgcttc gggaggacgg
     1861 atggtgtgga tcatgcttga caggtggcgc aagtgaaccg cgcgagcgcc gcggagtccc
     1921 gcgccggggt cgagctgacc aatctcgacc accccctgtt cgacggcgcg gacgcgacca
     1981 agcgggattt ggtggactat ctggacgctg tccgggaccg catcctgtgc gcccttcggg
     2041 accggccgct gtcagtgatc cgcgtgcggc caggccagca gccgttcatg cagaagaacg
     2101 tgccgaaata cgccccggat tgggtgcaga cgaccaccgt gtgggccgag gcgtcccggc
     2161 gtgaagtggc ctacgccctg tgtaatgacc ggcgcacgtt gctgtggttt gccaaccagc
     2221 gcgctgtgga ataccacccg acgttggtac ccgacggtgc cagccacccg acgcacctgg
     2281 tgctcgacct cgacccgccg gccggtgacg cgttcccgca tgtggtccgg gcagcacagc
     2341 tcgtgcggca ggccctggcc gacgctgggc tggcgggcgc ggtcaagacc agcgggtcga
     2401 agggcctgca cgtgttcgtg ccgctcgacg accgggtggg gatcgaggag gccgcagcgg
     2461 cgaccagggc ccttgccgca cgggccgagc ggctcgctcc cgacgttgcg acgacggcat
     2521 tcatccggga ggaccgcgac ggcaaagtgt tcctggactc cacccgctcc agcggtgcca
     2581 ccgtggtggc ggcctacagc ccgcgggccc gtcccggcg
//
LOCUS       DATADN010000782         1511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_81284, whole genome shotgun sequence.
ACCESSION   DATADN010000782 DATADN010000000
VERSION     DATADN010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81284"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VHH53_10175"
     CDS             complement(<1..1078)
                     /locus_tag="VHH53_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2290534.1"
                     /translation="MAEPGHDVLGLASALIGLARVRAVDDLAVAHSLAARAVALGEGL
                     REVPAYTTRGWVDLLAGDRQGAARDAERAASAARRRRDGPGLAEALALTVLSSADPTG
                     KLALLDEAIEILRECGCHLEEAAARVVSHRIGATIPDGDAILAEKTLRDAGVDIKSRR
                     AAGPLAVVVQFAPSVSIQALGSFRVMRDGVPVPKTAWKSKKARDLLKILVARRRPVPR
                     EQLMELLWPEVDRAKSGNRLSVLLSMVRDVLQPEQVPGEPLTTDGEAVGLDLGLVHVD
                     VEEFLTNAEIELAAHRDGHPDAIARLAAAVAVYTGDFLEDDPYQDWVTPLAEELRATY
                     VALLQALATRLRQAGDVDGVVRYTL"
     gene            complement(1091..>1511)
                     /locus_tag="VHH53_10180"
     CDS             complement(1091..>1511)
                     /locus_tag="VHH53_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290535.1"
                     /translation="ALAMLSAAEGDRRLADAHWRDALQASKEAADDLVQMGWIRCSRA
                     THVLDMGAPQQALIDAQIALDLGDRCENPFLTAHASTTRGKALVRLGMLDEALAAFGT
                     AIDLFQNLGSRFLGWPLCGLGDLYRTRGQLVRARGAY"
BASE COUNT          243 a          529 c          510 g          229 t
ORIGIN      
        1 gcagggtgta gcgcaccacc ccgtcgacgt cgccggcctg ccgcagccgg gtggccagtg
       61 cctggagcag cgcgacgtag gtagcgcgta gctcttcggc cagcggcgta acccagtcct
      121 ggtagggatc gtcctccaga aagtcccccg tgtagacggc caccgctgct gccagtcggg
      181 cgatcgcgtc cgggtgtccg tcccggtggg ccgcgagttc gatctcggcg ttggtgagga
      241 actcctcgac gtcgacgtgg accaggccga ggtccaggcc taccgcttcg ccgtcagtgg
      301 tcaatggttc acccggtacc tgttcgggct gcagcacgtc gcgcaccatg gacagcagca
      361 cggacagccg gttgccggac ttggcgcggt ccacctccgg ccagagcagc tccatgagtt
      421 gctcgcgtgg taccggtcgg cgccgggcga cgaggatctt cagcagatcg cgcgctttct
      481 tggacttcca ggctgttttc ggaaccggaa cgccgtcacg catcacgcgg aacgaaccca
      541 gcgcctggat ggacaccgaa ggcgcgaatt gcaccactac ggccaacggt ccagccgccc
      601 gccgggactt gatgtccacg ccggcatccc ggagtgtttt ctccgcgagt atggcgtcgc
      661 cgtcgggaat cgttgcgccg atgcggtggg acacgacgcg ggccgcggcc tcctcgagat
      721 ggcagccgca ctcccgcagg atttcaatcg cctcgtcgag cagggcgagt ttgccagtgg
      781 gatcggcgga cgacagcacg gtgagagcga gggcctctgc cagcccgggg ccatcgcggc
      841 gacgtcgggc tgctgacgcc gcccgctctg cgtcccgagc ggctccctga cggtctcctg
      901 ccaacagatc aacccaacca cgggtcgtgt aggcggggac ctcacgaagc ccttctccca
      961 gtgccaccgc ccgcgcagcg agcgaatgtg ccaccgcgag gtcgtcgacc gcccgcacgc
     1021 gtgcgaggcc gatcagcgca gaggccaggc cgagcacgtc atggcccggt tcagccaagg
     1081 caagggcctc ctagtatgca ccccgggcgc gaaccagctg gcccctggtt cggtacagat
     1141 cgccgaggcc acacaacggc cagccgagga accgggaacc gaggttctga aagaggtcga
     1201 tggccgtgcc gaacgctgcc agcgcctcgt ccagcatccc cagccgaacc aaggccttgc
     1261 cgcgggtggt cgaggcgtgc gcggtgagga acgggttctc gcaccggtcg cccagatcca
     1321 acgcaatctg ggcgtcgatc agcgcctgct ggggtgcccc catgtcgagc acgtgggtcg
     1381 cacgcgaaca tcggatccag cccatctgga cgaggtcgtc cgccgcttcc ttgctggcct
     1441 gtagggcgtc ccgccaatgg gcgtcggcga gccgccggtc gccctcggcc gccgaaagca
     1501 tcgccagcgc c
//
LOCUS       DATADN010000783         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_839564, whole genome shotgun sequence.
ACCESSION   DATADN010000783 DATADN010000000
VERSION     DATADN010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839564"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..270
                     /locus_tag="VHH53_10185"
     CDS             151..270
                     /locus_tag="VHH53_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2290536.1"
                     /translation="MHISLSTVKTHLASLMRKLDARNRVEIAMWAYETGRIKT"
     gene            complement(464..1270)
                     /locus_tag="VHH53_10190"
     CDS             complement(464..1270)
                     /locus_tag="VHH53_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290537.1"
                     /translation="MRAFSSEWVKLSRPATLFGFGGTMIGFTLLFTIMAFESAGGRNI
                     DLDVTTAMLSLPEGSILVVTDMAVFLGIVALALFASNLAGEFSKGTIRMLFVTEPNRL
                     KVLAGKILALTTFVAAGIAATLAFSIGGGALMANRVGVETAAWWTADGLAAIGAAYIN
                     ITAAAQVPALIGATIAVLTRSTTIAISVGLGWFILAEALIGALWDGLSRWGPAAVSTA
                     LAAGGAGGIGMIDGATPGISYATAILLAVGYSLLALTITSTVLARRDVTS"
     gene            complement(1461..2183)
                     /locus_tag="VHH53_10195"
     CDS             complement(1461..2183)
                     /locus_tag="VHH53_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2290538.1"
                     /translation="MASIRTAGLTKDFGDLRAVSEIDLDLPEGGVAGFVGPNGAGKST
                     TIRMLLGLIQPTSGDGEVLGHPIADPPGYLHRVGALIEAPAFYPTLTGQENLRVFAAL
                     GGHDRGRIAELLEVVGLRERGDDRYKNYSLGMKQRLGIALALLPDPDLLILDEPTNGL
                     DPQGIVEIRSLLMQLGARGKTVFVSSHLLAEIQAACDSIVIIDHGGVVFSGSMADLLA
                     RTRPPSVPQQTTPPIRPDSSSS"
     gene            complement(2188..2676)
                     /locus_tag="VHH53_10200"
     CDS             complement(2188..2676)
                     /locus_tag="VHH53_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HEX2290539.1"
                     /translation="MRTQRAELTALEDRQLPVQAKLAAAWTGVMFLYAYVDIIAFFKP
                     GVIDDILAGKVWEFDVSQPLLTTFLALMAIPIFMVVLSMTLPARANRITNLIVASVQV
                     PYAAFNVVGESWTYFYGLGVALEVIVLAVILRFAWTWPRTSSPSMPEPAAPHRPQLRM
                     GR"
BASE COUNT          515 a          939 c         1030 g          490 t
ORIGIN      
        1 gccgagcatc accgcgcggc tgctcgccgc cttcgcagac acccatgaag gagcgaaacc
       61 ggcgcagccc atcgaaccgc tcagcagccg gaggaggagg tcctgatccc ggtcgcacaa
      121 ggctggacca acagcgagat cgccgacgac ctgcacatca gcctgagcac cgtcaagacc
      181 cacctggcca gcctcatgcg caagctcgac gctcgcaacc gcgtcgagat cgcgatgtgg
      241 gcctacgaaa ccgggcgcat caagacctga cgacccacac cgaaaccgcc ggcggctgcc
      301 taggagtccg gctcacgcga cccgcagctc ctcatggtcg gagctggtcg atcgtgttga
      361 ggatgtgctc gtggccccgt tcgaactgct cggtgtagtc gtagtccgag gccccgagcg
      421 cggctgagcc ggagaaagct cgctgagcgg ctggcgcgct ctactagctg gtgacgtccc
      481 ttcttgcgag caccgtggag gtgatggtca gcgcgaggag gctgtagccg acggcgagga
      541 ggatggcggt ggcgtagctg atgcccgggg tggcaccgtc gatcatcccg atcccaccgg
      601 caccgcccgc cgccagggcg gtgctgacag cggccggacc ccagcggctc aatccgtccc
      661 agagcgcacc gatgagtgct tcggcgagga tgaaccaacc gagaccgacg gatatggcga
      721 tcgtcgtgga tcgggtgagc accgcgatcg tggccccgat gagggcaggg acctgggcag
      781 ctgccgtgat gttgatgtag gcagcaccga tggccgccag cccgtcggct gtccaccagg
      841 cggcggtctc gacaccgacc ctattcgcca tcagggcgcc accgccgatg ctgaaagcca
      901 gtgtggcggc gatcccggcc gcgacgaacg tcgtcagcgc aaggatcttg ccggcgagca
      961 ccttgagccg gttcggttcg gtcacgaaca gcatccggat ggtgcccttg ctgaactcgc
     1021 cggcgaggtt ggaggcgaag agggccagcg cgacgatccc gaggaacacg gccatgtcgg
     1081 tgacaacgag gatggagcct tccggcagcg acagcatcgc tgtggtgacg tcgaggtcga
     1141 tgttccggcc gcccgcgctc tcgaacgcca tgatcgtgaa caacagcgtg aagccgatca
     1201 tggtgccgcc gaacccgaac agtgtggcgg gacgagacag cttgacccac tctgagctga
     1261 aggcccgcat cagttggtcc cgtccttggt gtcgccggtc atggcgaaga aggctcgctc
     1321 cagatcggcg gaggtggcct ggagctcgcc gagggtgatc ccggcctgcc aggccatgcg
     1381 gttgaggtcg gcggtccagc gggctggcgc ggcgatctcg atggcgccgt tgacccgtgt
     1441 cgatgggtag ccggcagcag ctacgagctc gatgagtcgg gccgtatcgg tggcgttgtc
     1501 tgctgcggca cggatggtgg gcgagttcgg gcgagcaggt cggccatcga cccgctgaag
     1561 accacgccgc cgtgatcgat gatcacgatc gagtcgcagg ctgcctggat ctcagccagg
     1621 aggtggctcg agacgaacac ggtcttgcct cgagcaccaa gctgcatcag caggctgcgg
     1681 atctcgacga tcccttgggg gtccaacccg ttcgtcggtt cgtcaaggat cagcaggtct
     1741 ggatctggaa gcagcgccag agcgataccg agccgctgct tcatccccag cgagtagttc
     1801 ttgtaccggt cgtcaccgcg ctcgcgtagc ccgaccactt ccagcagctc ggcgatccga
     1861 ccgcggtcgt gcccgcctag ggcggcgaac acccgtaggt tctcctgacc ggtcagcgtc
     1921 ggatagaacg cgggcgcctc gatgagtgca ccgacccggt ggaggtagcc aggcgggtcg
     1981 gcgatggggt gacccaggac ctcgccgtcg ccgctggtcg gctggatgag gccgagcagc
     2041 atgcggatgg tcgtgctctt gccggcgccg ttagggccga cgaacccggc caccccaccc
     2101 tcgggtaggt cgaggtctat ctcgctgacg gcccgaaggt cgccgaagtc cttggtcaga
     2161 ccggctgttc ggatcgaagc catgggccta ccgtcccatc cggagctgcg gtcggtgtgg
     2221 tgcggcgggc tctggcatcg acggtgagga cgtgcggggc caggtccagg cgaagcgcag
     2281 gatgacggcg agcacgatca cttccagtgc gacacccagg ccgtagaagt acgtccagga
     2341 ctcgcccacc acgttgaatg ccgcgtaggg gacctgtacc gaagccacga tgaggttcgt
     2401 gatgcggttc gcccgggcgg gcagcgtcat cgacagcacg accatgaaga tcgggatcgc
     2461 catgagcgcg aggaaggtgg tcaacagcgg ctggctgacg tcgaactccc agaccttgcc
     2521 ggcgaggatg tcatcgatga cgccgggctt gaagaaggcg atgatgtcca cgtaggcgta
     2581 caggaacatg acgcctgtcc acgctgctgc gagcttcgcc tgaaccggaa gctgccggtc
     2641 ctccagcgcg gtgagctctg ctctctgggt tctcacgtcg ttgctccttc gtttcggtgg
     2701 gatggttggt tccaccacga tggctgacct cgtcggcggg cacatcagcc cgagggctcg
     2761 agttggcctg tccgaccgaa cggtctctgg ctcatacttt cggctgatgt gcaccaccca
     2821 cgcggcgctg cctcttgcgc ccggcgcgac gcgaggggac ggaccaacca tcaacaggcc
     2881 ggtgggggtg tcaggcgtcg ttcatctgcc tcgtcgtgcc tatgtcaact aggcggtgag
     2941 ttcaggcgtc gtcgtgggcg aggttgctgg acag
//
LOCUS       DATADN010000784         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1290856, whole genome shotgun sequence.
ACCESSION   DATADN010000784 DATADN010000000
VERSION     DATADN010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290856"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VHH53_10205"
     CDS             complement(<1..274)
                     /locus_tag="VHH53_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX2290540.1"
                     /translation="MARWVRAIRQGWVVDGGVPGPDVDEFAEATEVIEALASHVDGGD
                     TLLAVQHPHRTPEALADGLSLAAALPAARQTLDRLRASAYRRVDEVV"
     gene            complement(284..1273)
                     /locus_tag="VHH53_10210"
     CDS             complement(284..1273)
                     /locus_tag="VHH53_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX2290541.1"
                     /translation="MRSRLSAWYYALRTTNPPEGPVDAVTRWIVVTRAAVLPMTLVAG
                     LVAGLLAARAPGVDWRWLALAVVGIVLAHLANNLMNDLYDTSAGSDTVNYPRALYAPH
                     PILSGLVTRRALILSVLLVNAADLVILVALTLVRGWPIVAFALAGFVLSVAYTAPPLR
                     LKKRGLGEPDVLVVWGPLMVGGTYYAGVGSITWQVLLASLPYALLCTSVLMGKHIDKI
                     PYDAPLGIRTLPVLLGERRARAATLAMLAGFYLLVVICVVVGVLPWPALLALGGLYRL
                     ATVWPWFRQPRPDRPPRAVPVWPLWFAALAFVHLRVAGPLLVLGLAVAAIAGM"
     gene            complement(1378..1737)
                     /locus_tag="VHH53_10215"
     CDS             complement(1378..1737)
                     /locus_tag="VHH53_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX2290542.1"
                     /translation="MPQLPEPRPDGMVTAVDDLEEAMRDVVDPELGINVVDLGLVYDI
                     RVDDGNVAIVDMTLTSAACPLTDVIEDQTRAALTGGPGGGLVDDVRINWVWMPPWGPD
                     KITEDGREQLRALGFTV"
     gene            complement(1779..>2149)
                     /locus_tag="VHH53_10220"
     CDS             complement(1779..>2149)
                     /locus_tag="VHH53_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX2290543.1"
                     /translation="PTCGDEVTLRVALDGDRVADVSYEAMGCSISQAATSVLTDLVVG
                     RTVAESTSTMDAYMEMVQSRGQVEPDEDVLQDAVAFAGVARYPARVKCALLGWMAFKD
                     AVGRVDAGAADNGAKRAEAQ"
BASE COUNT          393 a          791 c          669 g          296 t
ORIGIN      
        1 ctaccacctc gtccacccgg cgatatgcgc tggcacgcaa ccggtccaag gtttgccgtg
       61 ctgccggcag cgcagccgcc aatgaaagcc cgtcagccaa cgcctcgggt gtgcggtgcg
      121 gatgttgcac agccagcaac gtgtcaccgc catcaacgtg gctggccagc gcctcgatca
      181 cctcggtggc ttcggcgaac tcgtcgacgt caggcccggg tacgccgccg tcaaccaccc
      241 agccttgcct gatcgcccgc acccaccgtg ccatccagct gtctcacatc cccgcgatgg
      301 cggccacggc caggccgagc acgagcaacg gaccggcgac ccgcagatgt acgaacgcca
      361 gcgcggcaaa ccacagcggc cagaccggca cggcacgtgg cggccggtca ggtcgaggtt
      421 gccggaacca cggccatacc gtggccagcc ggtacagccc gccgagtgcg agcagcgccg
      481 gccacggcag cacgccgacc accacgcata tcacgacgag caggtaaaag ccggccagca
      541 tggccagcgt cgcggcgcgg gcccgccgct cgccgagcag caccggcagc gtgcggatgc
      601 ccagcggcgc gtcataggga atcttgtcga tgtgcttgcc catcagcacc gacgtgcaca
      661 gcagcgcata aggcagcgag gcgagcagca cctgccaggt gatcgacccc actcccgcgt
      721 agtacgtgcc cccgaccatc agcggtcccc acacaacgag gacgtccggc tcgcccaaac
      781 cgcgcttctt cagccgcagc ggcggtgccg tataggccac cgacagtacg aaaccggcca
      841 gtgcgaacgc cacgatgggc cagccccgca ccagcgtgag ggcaacgagg atcaccaggt
      901 ccgcggcgtt gaccagcagc accgataaga tcagagcccg gcgggtgacc agcccggaga
      961 ggatcgggtg cggagcatag agcgcccgcg ggtaattcac ggtatccgaa ccggcgctgg
     1021 tgtcgtacag gtcgttcatc aggttgttgg ccagatgcgc cagcacgatc ccgaccacgg
     1081 ccagcgcgag ccaccgccag tcgacgccgg gggcacgggc ggcgagcaat ccggccacca
     1141 aaccagcgac cagcgtcatc ggcaatacgg cggcgcgggt caccacgatc caacgggtca
     1201 ccgcgtcgac cgggccctcg ggcggattgg tggtgcgaag cgcgtagtac caggcgctga
     1261 gccgggatcg caccgcagta tccgtcacac catcgagcct cgcacagctg cggtcgaatg
     1321 gtcagttcac acggtgaatc cacctcccgc actgtgcctc gcgcgggcga ggacgcgtca
     1381 gacggtgaaa cccagggctc gcagttgttc ccggccgtcc tcggtgatct tgtccggacc
     1441 ccacggcggc atccataccc agttgatccg cacgtcgtca accagtcccc cacctggacc
     1501 accggtgagc gcagcgcgag tctgatcctc gatgacgtcg gtcagcgggc acgccgcgga
     1561 agtcagcgtc atgtccacaa tcgcgacgtt gccatcgtcg acccggatgt cgtagaccaa
     1621 gcccaggtcg accacgttga tgccgagctc cgggtcgacc acatcgcgca tggcttcctc
     1681 gaggtcgtcg acggcggtca ccatcccgtc cgggcgcggc tccgggagct gaggcatccc
     1741 ggccgctccg cgttgcacct catgatcctc gttcaggctc actgtgcctc cgctcgcttg
     1801 gcaccattgt ctgcagcgcc ggcgtccacc cggccgacgg catccttgaa agccatccag
     1861 cccagcaagg cacacttcac ccgggccggg tagcgggcca cgccggcgaa ggcgaccgcg
     1921 tcctggagaa cgtcctcgtc gggctccacc tggccgcggc tctgcaccat ctccatgtac
     1981 gcgtccatcg tggatgtcga ctccgccacg gtccggccaa ccaccagatc ggtgagtacc
     2041 gatgtcgccg cctggctgat cgaacaaccc atcgcctcgt aagacacgtc ggccacccgg
     2101 tcgccgtcca gggcgactcg caaggtcacc tcgtcgccgc aagtggggt
//
LOCUS       DATADN010000785         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_406782, whole genome shotgun sequence.
ACCESSION   DATADN010000785 DATADN010000000
VERSION     DATADN010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406782"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VHH53_10225"
     CDS             <1..139
                     /locus_tag="VHH53_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005445473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEX2290544.1"
                     /translation="LAPADGQVVHAEFGCGAHSEVQLDTSPSVPVAELVYDDAELEIV
                     T"
     gene            268..>1577
                     /locus_tag="VHH53_10230"
     CDS             268..>1577
                     /locus_tag="VHH53_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290545.1"
                     /translation="MLVELAQNAADAAQRAAVPGYLWVTVEDGELRAANTGAPLDCAG
                     VAALAALRASAKREGGSVGRFGVGFAAVLGVTDAPQVISRSGAVAFSAQRTAAAVAGD
                     PALCAELHRRGGQPPVLRLVWPATGDPPPGFDTEVRLPLRPGIDPDALLAAFAAEAAD
                     LLLALPWLHRIDIGDRALRREGEDPVLIHYGSELHRWRVVRRNGLLPAAALVGLGAET
                     RPEWSLCWALPVGPDGTPLRLAGEVLHAPTPTAERLTLPARLIGTVPVDPSRRHVHPG
                     PAADLLLHTAAQAYPALAGVVAPDQRTALVPVPGFPAGEIDAVLRDAVVEALRHARWL
                     PGASGGQAVPVDAIVLDVALPELAELLADVTPNLLDAELAAPRHAAALAALGVTRLRP
                     AAIADLLAGLDRPPPWWRRCYAALLPLLDADPTVREELGALPVPL"
BASE COUNT          183 a          555 c          585 g          254 t
ORIGIN      
        1 gctggctccc gccgacgggc aggtggtgca cgccgagttc ggctgcggag cgcattccga
       61 ggttcagctg gacaccagtc ccagcgttcc ggtggccgag ctggtctacg acgacgccga
      121 gctggagata gtgacctgaa caccgacccg ttcggcaccg cagcgctgcg cgaaggggta
      181 ctcgcggcct ggcgggggtc tccgacgcgg ttccgggagg acgccaacac cgagtccgac
      241 ctcgctgcac ttggttacgc cgaccggttg ctggtcgaac tggcgcagaa cgccgccgac
      301 gccgcccagc gtgcggcggt gcctggatac ctctgggtca ccgtcgagga cggcgaactg
      361 cgggctgcga acaccggggc accactggac tgcgccgggg tggccgcgct ggccgcgctg
      421 cgcgcctcgg ccaagcggga aggtggcagc gtcggccggt tcggggtggg tttcgcggcg
      481 gtcctgggag tcaccgatgc gccgcaggtg atatcccgct ccggcgcggt cgcgttctcg
      541 gcgcagcgta cggccgccgc cgtggcaggt gatccggcac tgtgcgctga gctgcaccgc
      601 cgcggtggtc aacccccggt gctgcggttg gtgtggcccg ctactggtga cccgccaccg
      661 gggttcgaca ctgaggtccg gttgccactg cgtcccggga tcgaccccga cgcgctgctt
      721 gcggcgttcg cagccgaggc tgctgatctg ctgctggcgt tgccgtggct gcaccgcatc
      781 gacatcggtg atcgggcgct gcgccgcgag ggtgaggacc cggtgctgat ccactacggc
      841 tccgagctgc accggtggcg ggtggtgcgc cggaacgggc tgctacccgc ggccgcactg
      901 gtaggcttgg gcgccgagac ccggccggag tggagcctgt gctgggcact gccggtcggc
      961 cccgacggta ccccgttacg cctggcaggc gaggtgttgc atgcgcccac ccccaccgcg
     1021 gagcggctca ccttgcccgc ccggctgatc ggtacggtgc ccgtcgaccc gtcacggcgc
     1081 cacgtccatc ccgggccggc tgccgatctg ttgttgcaca ccgccgcaca ggcttacccg
     1141 gcgctggccg gtgtggtggc gccagatcag cgcaccgcac tggtaccggt acccggtttc
     1201 ccggccgggg aaatcgacgc ggtactacgc gatgcggtag tcgaggcgct gcgacacgcc
     1261 cggtggctgc ccggcgcgtc gggcgggcag gccgttcccg tcgacgccat tgtgctcgac
     1321 gtggcattgc ccgagctggc cgagctgctc gctgatgtga cgcccaatct gctcgacgcc
     1381 gagctggccg cgccccggca cgccgccgcg ctcgccgccc ttggtgtgac ccggttgcgt
     1441 ccagcagcga tcgctgacct gctggctggg ttggaccggc cgccgccgtg gtggcgccgt
     1501 tgctatgcgg cgttgctccc gctgctcgac gccgatccga cggtccgcga ggagctcggt
     1561 gcgttgccgg tgccgct
//
LOCUS       DATADN010000786         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_553477, whole genome shotgun sequence.
ACCESSION   DATADN010000786 DATADN010000000
VERSION     DATADN010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553477"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VHH53_10235"
     CDS             <1..233
                     /locus_tag="VHH53_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290546.1"
                     /translation="QIFAVNRSTEPVTLDIDLRALPAMRPNGQISLADDDSRKINSAD
                     APERVAPTRNTGGTTVTLPPISWHALSFEEQS"
     gene            230..>1543
                     /locus_tag="VHH53_10240"
     CDS             230..>1543
                     /locus_tag="VHH53_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2290547.1"
                     /translation="MNPHLTRRGLLGLGVGAGGAALLAACGDDGPNPVVGGGQNAQPA
                     ATSYDGPNVALAFWNGFTGGDGAFMKKLVDQFNTEHQNIKVTMNVYQWADYYQKVPAA
                     VATGNGPDLGIMHVDSVATNAARGVILPLDDLAKSLNLAEADFSPPVWQAGTYNNQRY
                     AIPLDVHPLGLFYNKALMEKAGLDPEKPPATNDEYMAALDKMKTAGIQGSWATPFQFT
                     GSMWFQSLLWQFGGDLFDQNAQTARFAEEPGVQALTWYVDLVNKGYSPKNVAQDADIV
                     ALRNGKNAFNWNGIWTINTLKEDAKLQWGAAPVPQIGTQKAVWAGSHQFVQYKQRQKD
                     DNKLTAGKVFMNWIGQQSLEWAKGGQVPARKEVRESAEFKALPEQAALAQQIDYVHYP
                     PSVPGIGDALNTVNTAVNEAVLLRKPPAKALSDAAAKANQILEQNR"
BASE COUNT          304 a          525 c          507 g          207 t
ORIGIN      
        1 tgcagatctt cgcggtgaac cggagcaccg agccggtcac cctcgacatc gacctgcggg
       61 cattgccggc tatgcgcccc aacgggcaga tcagccttgc cgacgacgac tcgcggaaga
      121 tcaattccgc ggacgcgccg gagcgggtcg cccccaccag aaacaccggc ggtaccaccg
      181 tcacccttcc cccgatttcc tggcacgcct tgagtttcga ggagcaatcg tgaatccaca
      241 cctaacccgc cgcgggctgc tcggcctcgg cgtgggcgcc ggcggcgccg ccctgctcgc
      301 cgcgtgtggc gacgacggac cgaatcccgt ggtcggcggt gggcagaacg cccagccggc
      361 cgccacctcg tacgacggcc cgaacgtggc gctggccttc tggaacggct tcaccggtgg
      421 tgacggcgcg ttcatgaaga agctggtcga ccagttcaac accgaacatc agaacatcaa
      481 ggtcacgatg aacgtgtacc agtgggccga ctactaccag aaggtgcccg ccgcggtggc
      541 caccggcaac ggtcccgacc tgggcatcat gcacgtcgac agcgtggcca ccaacgccgc
      601 ccgcggggtg atcctgccgc tggacgacct ggccaagtcg ctcaacctgg ccgaggccga
      661 cttctcgcca ccggtgtggc aggccgggac gtacaacaac cagcggtacg cgattccgct
      721 ggacgtacac ccgctgggcc tgttctacaa caaggccctg atggagaagg ccggcctgga
      781 cccggagaag ccgccggcca ccaacgacga gtacatggcc gcgctggaca agatgaagac
      841 ggccggcatc cagggcagct gggccacccc gttccagttc accggcagca tgtggttcca
      901 gtcgctgctg tggcagttcg gcggtgacct cttcgaccag aacgcgcaga ccgcgaggtt
      961 cgccgaggag ccgggcgtgc aagcgctgac ctggtacgtc gacctggtca acaagggcta
     1021 cagcccgaag aacgtggccc aggacgccga catcgtggcg ctgcgcaacg gcaagaacgc
     1081 cttcaactgg aacggcatct ggaccatcaa caccctcaag gaggacgcga aactccagtg
     1141 gggtgcggcg ccggtgccac agatcggcac tcagaaggcc gtgtgggcgg gctcgcacca
     1201 gttcgtgcag tacaagcagc ggcagaagga cgacaacaag ctgaccgccg gcaaggtgtt
     1261 catgaactgg atcggccagc agtcgctgga atgggccaag ggtggtcagg tgccggcccg
     1321 caaggaggtc cgggagagcg ccgagttcaa ggccctgccc gagcaggcgg cgctggccca
     1381 gcagatcgac tacgtgcact acccgccgtc ggtgccgggc atcggtgacg cgctgaacac
     1441 cgtcaacacc gcggtgaacg aggcggtcct gctgcgtaag ccgcccgcga aggcgctgtc
     1501 cgacgcggcc gccaaggcga accagatcct cgagcagaac cgc
//
LOCUS       DATADN010000787         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1160261, whole genome shotgun sequence.
ACCESSION   DATADN010000787 DATADN010000000
VERSION     DATADN010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160261"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VHH53_10245"
     CDS             <1..1698
                     /locus_tag="VHH53_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX2290548.1"
                     /translation="VWTEPSKKLRSVHTPLDVADARRIAAAIADRGYDSVAVAFLHSY
                     ANPTHELQMREVLRELAPDAEVTLSHELSREYREYERTSTAVLDAYIKPIVRRYLRSL
                     EDQLAEGGFIGRFLMTRSGGGAMTATSAREQPVNLILSGPAGGVIGAAAFSRLIDQPN
                     LITIDMGGTSLDASLILDSEPVLYRGAEFEQLPINTPSLYIHTIGAGGGSIAWLDAAG
                     ALQVGPQSAGAMPGPASYGRGGTQPTFTDAALLLGYLGTDTPLGGSLVLDVALAEQAL
                     QPLADELGMSVTALARGILRISTTKIMGAVRAITVELGRDPKDFALLSFGGGGGLVAV
                     DVARELGIPTVVVPPGQGAFSAFGMLMADVQHDLARTSVTSLAELDLAAVEAAYKEMH
                     SEAQALLTEEGFGAERQHVVRTVDVRYAGQEHSVTVAFPSGNGEVVASITEEFTRLHE
                     RQYGHTMEDPIEITTLRLRATGSVDKPSLPLLPKRVTGHPKAHGRRAVYLSPDRPAVD
                     YALFLREELLAGDVITGPAVIAEHTATTVMHAGEVLRVGRHGEMVLKVSTRPEEAWR"
     gene            1695..>2803
                     /locus_tag="VHH53_10250"
     CDS             1695..>2803
                     /locus_tag="VHH53_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX2290549.1"
                     /translation="MSDSITTEIIRHGLLAVAEEMARNLSRTAYNTVVYEIHDYGIGI
                     HDPNGDVVADAPGIAVFTRGNDHGIKNAVEFLGVDAMQPGDIFILNYPYWSSAHTLDP
                     LVFAPIHHDSRLIGFASCRVHVLDLKQKDPGYVLDSTDMSQEGIFFPATRLYRAGARN
                     DDVFNIIRFNSRMPERTIGDIQAQVSACVTGVRRTQEIAAKYGADFLLAAMDAINDHG
                     ERLARLALARLPKGTWSAHDFVDTDGVDRDRMIRLHVTVTITDEEMVIDWTGSDREVR
                     GPINMPYGGTQAFCSLVFKALTTPDTPVVAGNFRPMRLVTEPGSVVHAVPPMPTFTLW
                     TGLLGGEVVLKALAQGIPDQVPACSGGDVCSMMGLG"
BASE COUNT          454 a          984 c          922 g          443 t
ORIGIN      
        1 gtatggaccg agccgtcgaa aaagctgcgc tcggtccata ctccgctgga tgtcgcggac
       61 gccaggcgca tcgcggcagc catcgccgac cgcggctacg actccgtcgc ggtggcgttt
      121 ctgcactcct acgccaaccc gacccacgag ctgcagatgc gcgaggtact tcgggagctt
      181 gccccggacg ccgaggtcac cctctcgcac gagctgtccc gcgagtatcg ggagtatgag
      241 cgaaccagca ccgccgtcct tgacgcctat atcaaaccaa tcgtgcgccg ctacctgcgg
      301 tcgctggagg accagctggc cgaaggcggc ttcatcgggc gcttcctgat gacccgctcg
      361 gggggtgggg cgatgaccgc cacctcggcg cgggagcagc cggtcaacct gatcctctcg
      421 ggccccgccg gcggcgtgat cggggcggcc gccttctcac gcctcatcga tcagccaaac
      481 ctgatcacca tcgacatggg gggcaccagc ctggatgcgt cgctgattct tgactccgag
      541 ccggttctgt accgcggcgc ggagttcgag cagctgccga tcaacacgcc gtcgctctac
      601 atccacacga tcggcgcggg cggcgggtcg atcgcatggc ttgatgcagc cggtgcgctg
      661 caggtcggcc cccagagcgc tggagcgatg cccgggccgg cgtcgtacgg ccgcggcggt
      721 acccagccca cgttcactga cgccgccctt ctgctcggtt acctgggcac cgacaccccg
      781 ttgggcggca gcctggtgct ggacgtcgcg ctcgctgagc aggccctgca gcccctcgct
      841 gacgagcttg ggatgtcggt gacggccctg gcgcggggca tcctccgcat ctccaccacc
      901 aagatcatgg gcgccgtgcg cgccattacc gtcgagctgg gcagggaccc caaggacttc
      961 gcgctgctgt catttggcgg cggaggaggc ctggtcgccg tcgacgtcgc tcgcgagctg
     1021 gggattccga cggttgtggt gccgcccggc cagggggcgt tctcggcgtt cgggatgctc
     1081 atggccgacg tgcagcacga cctcgcccgc acgtccgtca cctccctggc ggaactcgac
     1141 ctggccgcgg tggaggccgc ctacaaggag atgcacagcg aagcccaggc gttgctcacc
     1201 gaggaggggt tcggcgcgga gcggcagcac gtggtgcgca ccgtggacgt gcggtacgcc
     1261 ggccaggagc actcggttac cgtcgccttc ccctccggga acggggaggt ggtcgcctcc
     1321 atcacagagg agttcacccg tctccacgag cggcagtacg ggcacaccat ggaagacccg
     1381 atcgagatca cgacgctgcg gctgcgggcc accggctcgg tggacaagcc ctccttgcca
     1441 ctgctgccga agcgggtcac cggccacccg aaggcacacg ggaggcgagc ggtatacctg
     1501 tccccggacc gccccgccgt ggattacgcg ctgttcctgc gcgaggaact cctcgccggt
     1561 gacgtcatca ccgggcccgc ggtgatcgcg gaacacaccg cgacgacggt gatgcacgcc
     1621 ggcgaggtcc tgcgggtcgg ccgccacggc gagatggtgc tcaaggtcag cacccggccc
     1681 gaggaggcct ggcgatgagc gattccatca ctaccgagat catccgccac gggctgctcg
     1741 ccgtcgccga ggagatggcc cgcaatctgt cccggaccgc atacaacacg gtcgtctacg
     1801 agatccatga ctacggcatc gggatccacg acccgaacgg cgatgtcgtc gcggacgcgc
     1861 ccggcatcgc ggtgttcacg aggggcaacg atcacggcat caagaacgcg gtcgagttcc
     1921 tgggcgtgga cgcgatgcag cccggtgaca tattcatctt gaactaccca tactggtcgt
     1981 cggcccacac cctggacccg ctggtgtttg cgcccatcca ccacgacagc cggctcatcg
     2041 ggtttgcgtc ctgccgggtc cacgtgctcg acctgaagca gaaggacccc ggctacgtgc
     2101 tggactccac cgacatgtca caggagggca tcttcttccc ggccaccagg ctgtaccgcg
     2161 ccggagcccg caacgacgac gtgttcaaca tcatccgctt caacagtcgc atgcccgagc
     2221 gcaccatcgg cgacatccag gcccaggtgt cagcctgtgt caccggtgtg cgccgtaccc
     2281 aggagattgc cgccaagtac ggcgccgact tcctcctcgc cgccatggac gcgatcaacg
     2341 accatgggga gcggctggcc cggctggccc tggccaggct gcccaagggg acctggagcg
     2401 cccatgactt cgtcgacact gacggcgtcg accgggaccg catgatcagg ctgcatgtca
     2461 cggtcaccat cactgacgag gagatggtca tcgactggac cggcagcgac cgggaggtca
     2521 ggggtcccat caacatgccc tacggcggga cgcaggcctt ctgcagcctg gtcttcaagg
     2581 ccctgaccac ccccgacacc cccgtggtag ccggcaactt ccggccgatg cgacttgtca
     2641 ccgagcccgg gtcggtggtg cacgccgtcc cgccgatgcc caccttcacg ctctggactg
     2701 ggctgctcgg cggcgaggta gtgctcaagg ccctggccca gggcattccg gaccaggttc
     2761 cggcctgctc aggcggtgac gtctgctcga tgatggggct ggg
//
LOCUS       DATADN010000788         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1160317, whole genome shotgun sequence.
ACCESSION   DATADN010000788 DATADN010000000
VERSION     DATADN010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160317"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1097
                     /locus_tag="VHH53_10255"
     CDS             159..1097
                     /locus_tag="VHH53_10255"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX2290550.1"
                     /translation="MAPGTLATMLGSMHEWWVCLLEPDGLLLRDRALAAVDPDDLLTA
                     LRSGRLRRIQRGIYLPRRVEATPAVFARAAQLSSGAPGALPSHATAARVHRIAVPEDD
                     HVQHVTVTRDLRRRNRRDLHFHTRSIGCGEAERRDGVPVTSVARTLADVAASLDRLAA
                     VWAIDDALRRQLCAKTDIIAVIQRWRGGAGCVMARQRLDEADGVAESILETAGRLALL
                     DRGVPLPIPQYQLWAPDGTLVARLDGAYLREKVALEFDGADPHSLLEAVFRDRWRQNG
                     LPELGWTVLRFTWWDVMRDPDGVAEIVQRTLRRRAA"
     gene            1186..>1881
                     /locus_tag="VHH53_10260"
     CDS             1186..>1881
                     /locus_tag="VHH53_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEX2290551.1"
                     /translation="MAVTSVFEQLELLLPRISKPVQYVGGELNATVKDWDATTVRWAL
                     MYPDAYEVGVPNQGIMILYEVLNEQPDVLAERTYAVWPDLEALMREHGVPQFTVESHR
                     PVGAFDVLGVSFATELGYTNLLTALDLAAIPLHAPQRTDDHPVVIAGGHAAFNPEPIA
                     DFIDAAVLGDGEEAVLEITAVVRDWKAQGRPGGRQELLGRLAEIDGIYLPGCYDVSYL
                     PDGRVGQVISTGRA"
BASE COUNT          306 a          622 c          636 g          317 t
ORIGIN      
        1 ttcggcaccg tggtcgcctc ccgccgcctg cggttcctcc tgctccggta gggcgctctt
       61 caaggcctac caacccgctc ccagcgagcc cacaacgact accaacccca aggctgtcca
      121 caggcgagac cgttatccac agcaacgagc gttgccgctt ggcgcctgga acgctggcca
      181 ccatgctcgg ctccatgcat gagtggtggg tttgccttct ggagccagat gggctcctgc
      241 tgcgtgatcg agctctggct gccgtcgacc ccgacgacct gttgaccgcg ttacggtctg
      301 gccggttgcg gcgcattcag cgaggcatct atctgcccag acgggtcgag gcgacgccgg
      361 ccgtgttcgc gcgtgccgcg caactcagct cgggcgcacc aggagcgctg cccagccacg
      421 cgaccgcggc gcgggtacac cggatcgcgg tgcctgagga tgatcatgtg cagcacgtga
      481 cagtgacccg agatctgcgg cgacgtaatc gccgtgacct gcactttcat acccggtcga
      541 tcggctgtgg cgaggcggag cggcgagacg gtgtgccggt gacgtcggtt gcccggacgc
      601 tggccgacgt ggcagcgagt ctggatcggc tggccgcggt atgggcgatc gacgacgctc
      661 tgcgccgaca gctatgcgcc aagacggaca tcatcgccgt cattcagcgc tggcgcggtg
      721 gggccggttg cgtcatggcg cggcagcggc tggacgaggc ggacggcgtg gcggagtcaa
      781 tcctggagac tgcgggtcgc ctggcgttgc tcgatcgtgg ggtacccctg ccgatcccgc
      841 agtaccagct atgggcgccg gacggcacgc tggtggcccg gctcgatggt gcttacctgc
      901 gagagaaggt cgccctcgag ttcgacggtg cggatccgca cagcctgctc gaagccgtat
      961 tccgcgaccg ttggcggcag aacggcctgc ctgaactcgg ttggacagtg ctgcgcttca
     1021 cgtggtggga cgtgatgcgc gaccctgacg gggtggccga gatagtgcag cgcaccctac
     1081 gccgacgagc ggcgtgaagc ccaaccacac agggttggta gcagttaacg gtccaatcag
     1141 cccaggttgg tagtccttga agggcggcca actaacctga ccggtatggc agtcacgtcg
     1201 gtgttcgagc agctggaatt gctgctgccc cggatctcca agccggtgca gtacgtggga
     1261 ggcgagctca acgccactgt gaaggactgg gacgccacca cggtgcgctg ggccttgatg
     1321 tacccggatg cctacgaggt cggcgtgccc aaccagggaa tcatgatcct ctacgaggtg
     1381 cttaacgagc agcccgacgt cctcgccgaa cggacctacg cggtctggcc cgacctcgaa
     1441 gcactgatgc gtgagcacgg ggttccccag ttcaccgtgg agtcgcaccg gccggtcgga
     1501 gctttcgatg tgctcggagt ctcctttgcg accgaactcg ggtacacgaa cctgctcacc
     1561 gcgttggacc tggccgccat cccgttacac gccccacagc gcaccgacga ccatcccgtc
     1621 gtcatcgccg gtggccacgc cgccttcaac ccggagccga tcgccgactt catcgatgcc
     1681 gcggtcctgg gcgacggcga ggaagccgtc ctcgagatca ccgcggtcgt gcgggactgg
     1741 aaggcccaag gtcgcccagg cggccgccag gaactgctgg gccggctggc cgagatcgac
     1801 ggtatctacc tgccgggctg ctacgacgtc agctacctgc ccgacggccg ggtcgggcag
     1861 gtcatcagca caggacgcgc t
//
LOCUS       DATADN010000789         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_81931, whole genome shotgun sequence.
ACCESSION   DATADN010000789 DATADN010000000
VERSION     DATADN010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81931"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VHH53_10265"
     CDS             complement(<1..655)
                     /locus_tag="VHH53_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2290552.1"
                     /translation="MATATETRPPATQARQPAGGGSRGPSLASRAGAYAGLLLLTVLF
                     VSPLVFMLTTSFKTNFEAAQPTWIPKDPTTEGYSFIFSATQTPVLRWFANSLLAAIGQ
                     TLLILATASMAAYALARMEFPGKRLLTGLVIATIFIPPIVLLIPNYVIVSNLGWLDSL
                     AAVIVPGAAGAFGVFFLRQFFVSLPRELEEAAVLDGASRFQIFYKVILPLSKPALATL
                     "
     gene            complement(657..1475)
                     /locus_tag="VHH53_10270"
     CDS             complement(657..1475)
                     /locus_tag="VHH53_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2290553.1"
                     /translation="MLAPSVYVIWISLHDWDFLLPGKPFVGLQNYINLFKPGTVTSGP
                     FWQSMEATGKFVLFSVPLLVVIPLAVAVMLNQKFPGRNFFRASFFAPYVLGVAVVGIL
                     WRFLLDPNIGLVNHLLDRIGIDANVPWTTQTPWVWAALVGPTVWWTLGFNTVIYLAGL
                     QDIPRELYEAAKVDGANRWQQFRNVTIPGLRPVLVFVITITILASANVFGQPYLITQG
                     RPGFETRSAIMYIGQEGLREFRMGNAAAMSFLLTLALMLISVANFVFFRERKER"
BASE COUNT          289 a          515 c          595 g          234 t
ORIGIN      
        1 ccagggtggc cagggcgggc ttggacaggg gcaggatcac cttgtagaag atctggaagc
       61 ggctggcccc gtcgagcacg gccgcctcct ccagctcccg cggcagggag acgaagaact
      121 ggcgcaggaa gaacactccg aaggcgccgg ccgcgccggg cacgatcacc gccgccaggc
      181 tgtccagcca gccgaggttg gagacgatca cgtagttggg gatcagcagc acgatcggcg
      241 ggatgaagat ggtggcgatc accagccccg tcagcagccg cttgccaggg aactccatcc
      301 gggccagggc gtaggcggcc atcgaggcgg tggccaggat cagcagcgtc tggccgatgg
      361 cggccagcag gctgttggcg aaccagcgca gcaccggcgt ctgggtggcg ctgaagatga
      421 agctgtagcc ctcggtggtc gggtccttgg ggatccaggt cggctgggcg gcctcgaagt
      481 tggtcttgaa cgaggtggtg agcatgaaca ccagcgggct gacgaacagc acggtcagca
      541 gcagcagccc ggcgtaggcg ccggcccggc tggccagcga ggggcccctg gagccgccgc
      601 cggccggctg gcgcgcctgg gtggccggcg gcctggtctc ggttgccgtc gccatgtcag
      661 cgctccttgc gctcgcggaa gaagacgaag ttggccacgc tgatcagcat cagcgccagc
      721 gtcagcagga agctcatggc ggccgcgttg cccatgcgga actcgcgcag cccctcctgg
      781 ccgatgtaca tgatcgccga gcgggtctcg aaccccggcc ggccctgggt gatcaggtac
      841 ggctggccga acacgttggc cgaggccagg atcgtgatgg tgatgacgaa caccagcacc
      901 ggccgcagcc cggggatggt gacgttgcgg aactgctgcc agcggttggc cccgtcgacc
      961 ttggccgcct cgtacagctc cctggggatg tcctggagcc cggccaggta gatcacggtg
     1021 ttgaagccga gcgtccacca caccgtcggg ccgaccaggg ccgcccacac ccacggggtc
     1081 tgggtggtcc aggggacgtt ggcgtcgatg ccgatccggt ccagcaggtg gttgaccagg
     1141 ccgatgttgg ggtcgaggag gaaccgccag aggatgccga ccacggccac gccgagcacg
     1201 tagggggcga agaatgacgc ccgaaagaag ttccgcccgg ggaacttctg gttcagcatc
     1261 acggccacgg ccagcgggat gaccaccagc agggggacgc tgaacagcac gaacttgccg
     1321 gtcgcctcca tcgactgcca gaacggcccg ctggtcaccg tcccgggctt gaacaggttg
     1381 atgtagttct gcagcccgac gaacggcttg ccgggcagca ggaagtccca gtcgtgcagg
     1441 ctgatccaga tgacgtagac ggacggggcc agcacgaaga ggccgaacag gatcaggtag
     1501 ggggcgagga acaggtacgg gacgatgccg ccgctgttcg gcctcttcgt gctggccccg
     1561 tccgtctacg tcatctggat cagcctgcac gactgggact tcctgctgcc cggcaagccg
     1621 ttcgtcgggc tgc
//
LOCUS       DATADN010000790         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1010515, whole genome shotgun sequence.
ACCESSION   DATADN010000790 DATADN010000000
VERSION     DATADN010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010515"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VHH53_10275"
     CDS             complement(<1..190)
                     /locus_tag="VHH53_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290554.1"
                     /translation="MSMTVRRVVSITVAVAIIAFAAATLLSPIRAEVGGPIGIAFWIA
                     LTLIASALAVKLPNGVVAN"
     regulatory      complement(440..522)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            755..>1513
                     /locus_tag="VHH53_10280"
     CDS             755..>1513
                     /locus_tag="VHH53_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2290555.1"
                     /translation="MADHASYGQHAAHQDAPEQHAGAGDQAHDAHGGGHGKHAGHDPD
                     AFRRQFWILLVLTIPVVIWSMEVQEWLGYTAPQFIGSEWSPAVLGTVIFVYGGRVFLT
                     GARTELGDRQPGMMTLISLAIVVSFIASWAATLSLFAVDVWWELATLITIMSLGHWLE
                     MRSIMQAQGALSALAELLPDTAERIALDGAVESVPLHALSVGDLVLVRPGTRVPADGE
                     VAEGAADVDESMITGESRPVGKEAGDTVVAGTVAA"
BASE COUNT          275 a          485 c          504 g          249 t
ORIGIN      
        1 cgttagcgac gacgccgttg ggaagcttta cggcaagggc gctcgcaatg agggtgagcg
       61 cgatccagaa ggcaattcct atcggaccac cgacttcagc cctgatcgga gaaagcagcg
      121 tagccgccgc gaacgcaatg atcgcgacgg ctacggttat ggaaactaca cggcggacag
      181 tcatagacac cggtcgcgtc accctaggtg aacgcgccgg agtctaccac gcgccgatta
      241 ccagcccttg agcgacgctc cgccggcaaa agccaatgtg aggaggccga gtagggcagt
      301 aagcagggcg gagagccgct gaacgaagcg cttcatcttg cgacctccac gttcagggct
      361 accagccctt gagtgagttg cgagccatca gaaggtgccc tcttctgctg tcagatgcca
      421 tacaaccgac acactccggt gacggaacgg tcggttgcac tacatgctcc ccgacgacct
      481 aagtgcccac attcggtcgg cggatcgaag caccctcggt attgctaggg gggtaccaaa
      541 gtaccaatcc ctgtccggta ctctaggcgc cttggctggc aaagtcaacc cccatacgcg
      601 ccgcaggaac ggctggtcaa ccgatacttg acctacgttc ggcagccatt ggaccggcgg
      661 tgagcctgag ctcctcagat ggcgcctcat cgaactccgt cccaggatct gccgctgcac
      721 atgggcgatt gacgggcggc ccagagtagg ggtgatggcc gatcacgcct cctacggaca
      781 gcacgctgcc catcaggacg ctcctgaaca gcacgcgggc gccggggacc aggcacacga
      841 cgcccacggc ggagggcatg gcaagcacgc cgggcacgac cccgacgcgt tccggcggca
      901 gttctggatc ctcctggtgc tgacgatccc ggtcgtcatc tggagcatgg aggtccagga
      961 gtggctgggc tataccgcac cgcagttcat cggctccgag tggagtccgg ccgtcctggg
     1021 caccgtgatc ttcgtgtacg gcggccgggt cttcctgacc ggggctcgta ccgagctggg
     1081 cgatcgccag ccggggatga tgaccctgat cagcctggcc atcgtggtgt ccttcatcgc
     1141 gtcctgggcg gcgacactaa gcctctttgc ggttgacgta tggtgggagc tggccaccct
     1201 catcaccatc atgagcctcg gccactggct ggagatgcgc tcgatcatgc aggcccaggg
     1261 cgcgctgagc gcgttggccg agctgctgcc ggacaccgcg gagcgcatcg ctttggatgg
     1321 cgcggtggaa tccgtgccgc tgcacgcgct gtcggtaggt gacctggtcc tggtgcgacc
     1381 cgggacgcgg gtgccggccg acggcgaggt cgccgagggc gccgccgacg tcgacgagtc
     1441 catgatcacc ggcgagtcgc gcccggtcgg caaggaggcg ggcgacaccg tggtggcggg
     1501 caccgtggcc gcc
//
LOCUS       DATADN010000791         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1291783, whole genome shotgun sequence.
ACCESSION   DATADN010000791 DATADN010000000
VERSION     DATADN010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291783"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VHH53_10285"
     CDS             complement(<1..226)
                     /locus_tag="VHH53_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX2290556.1"
                     /translation="MSFHPYLNVALQGLEEVAGVRYEASDPARTKRGSPALVVYADDM
                     VALCHSRQQAEQVKARMAEWLAPRGLSFNEA"
     gene            598..2247
                     /gene="istA"
                     /locus_tag="VHH53_10290"
     CDS             598..2247
                     /gene="istA"
                     /locus_tag="VHH53_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX2290557.1"
                     /translation="MVDVTEIFIHWYAGRSISEVSASLGVDRKTIRKYLAPAVAGGLR
                     PGGAAMGQQDWATLVAGWFPQLADTRLRQTTWPQIAEHHEYITAQLKLGVTQATIHQR
                     LRDERGLTASVASLKRYVAANLPEEVRRDRVVVLRDESDTPPGEEAQLDYGHLGYWTD
                     SATGKRRRVWAFAMVLACSRHMFVWPVLTMDQAAWTQAHVEAFAFFGGVPRRLVPDNL
                     RTGVDKPDLYDPKLNRSYGELAAHYGVLVDPARRGKPRDKARIERPMPYIRDSFWRGR
                     QFTSLAEMQAAALVWCTEVAGQRSSRPLGGGSPASVFAAVEAPVLQPLPVKPFVLATW
                     SAAMVGPDIHAKVGKTIYSVPWRFIGQRVDARSTTTMVQIFHNGQLIATHGRKPQGKQ
                     TDLGHYPPEKIAFRMRTPTWCRTRAEQIGPACVQVIAGMLEVNALFRLRAAQGVIGLA
                     DKHGTQRLEAACAKAIAVGDPSYRTVKGILAAGAEGEPAAPSAGDGGAAAHLHGPSQL
                     FANVVALTTAGAADEAATATANDHHLRQGDGEQPTASGTVA"
     gene            2249..2446
                     /locus_tag="VHH53_10295"
     CDS             2249..2446
                     /locus_tag="VHH53_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290558.1"
                     /translation="MAHSAATPQPAYPLPRPATDARFTVGLTLDVAAVLTRHGYPPLT
                     CGGDLLRLQQALFTLIYQENR"
BASE COUNT          387 a          880 c          881 g          402 t
ORIGIN      
        1 tggcctcgtt aaacgacaga cccctcgggg ccagccactc ggccatgcgc gccttgacct
       61 gctcggcctg ctgccgtgag tggcagagag cgaccatgtc gtcggcataa accaccaggg
      121 ccgggctgcc tcgcttcgtg cgagccggat cgctcgcctc gtagcggact ccggccactt
      181 cctccagccc ctgcagcgca acattcaaat aggggtgaaa tgacaccgcc ttatgagcag
      241 tggcgggtta tgcgcagtgc tggcctgctg ggcctggtga cagcgatgcc gggggtcaat
      301 cgctgcgcat aatccgcaga tgatcgcgat caggcggtcc ggcatggtgc cgggccagca
      361 tgatcggagg tcagctgtta tggcggtttt gtctgcgttg gtggcgtgtc ggcgctcacg
      421 gatttcccca tttgtagcgg gttgacgctc acgtaattcc ccacccgggc gctcacgtaa
      481 ttccccacct aggtgctggg gtgcctaggg gtctcggtgg ggtggtcctc gcgatcatgc
      541 agagtccttc ctcgtcttgg acgggcgagg gagccgatgg cgaggagaac gttcgacgtg
      601 gtcgatgtga ccgagatctt catccactgg tacgcgggtc gttcgatcag cgaggtgtcc
      661 gcgtcgctgg gtgtggaccg taagacgatc cgtaagtatc tggcgccggc ggtggcgggg
      721 gggctgcgtc ctggtggcgc ggcgatgggc cagcaggact gggcgacgct ggtggccggc
      781 tggttcccgc agctggccga caccaggctg cggcaaacga cctggccgca gatcgccgaa
      841 caccacgagt acatcaccgc ccagctgaag ctgggcgtga cgcaggcgac gatccaccag
      901 cggttgcgcg atgagcgcgg cctgaccgcc agcgtggcgt cgctgaagcg gtacgtggcg
      961 gcgaacctgc ccgaggaggt gcgccgggat cgggtggtgg tgctgcggga cgagtccgac
     1021 acccctccgg gtgaggaggc tcagctcgac tacgggcatc tgggctactg gaccgactcg
     1081 gccaccggca agcggcggcg ggtgtgggcg ttcgcgatgg tgctggcctg ctcgcggcac
     1141 atgttcgtgt ggccggtgct gaccatggat caggccgcgt ggacgcaggc gcatgtggag
     1201 gcgttcgcgt tcttcggcgg tgtgccgcgc cgcctggtcc cggacaacct gcgcaccggg
     1261 gtggacaagc ccgacctcta cgacccgaag ctcaaccgct cgtatggcga actggccgcc
     1321 cactacggag tgctggtcga cccggcccgg cgcggcaagc cgcgggacaa ggcccgcatc
     1381 gagcggccga tgccctacat ccgggactcg ttctggcggg gccggcagtt cacctcgctg
     1441 gccgagatgc aggccgccgc gttggtctgg tgcaccgagg tggccggtca gcgctcgagc
     1501 cgaccacttg gcggcgggtc gcccgcgtcg gtgttcgccg ccgtcgaggc gccagtgctg
     1561 caaccgctgc cggtcaagcc gttcgtcctt gcgacctggt cggcggccat ggtcgggccg
     1621 gacatccacg ccaaggtcgg taagacgatc tactcggtgc cgtggcggtt catcggccaa
     1681 cgtgtcgacg cccggtccac cacaaccatg gtgcagatct tccacaatgg acaattgatc
     1741 gccacgcacg ggcgcaagcc gcagggcaag cagaccgacc tgggccacta cccaccggag
     1801 aagatcgcgt tccggatgcg gaccccgacc tggtgccgca cccgcgccga gcagatcggc
     1861 cccgcctgcg tgcaggtgat cgccggcatg ttggaggtca acgccctgtt ccggctgcgc
     1921 gccgcccagg gggtgatcgg cctggccgac aagcacggca ctcagcggct ggaagccgcg
     1981 tgcgccaagg ccatcgcagt cggcgacccg tcctaccgca ccgtcaaggg catcctggcc
     2041 gccggtgccg agggcgagcc ggccgcgccg tcggccggcg atggtggcgc cgcggcgcac
     2101 ctgcacggtc cttcgcagct gttcgccaac gtcgtcgccc tgaccaccgc gggcgcggcg
     2161 gacgaggcgg ccacggccac cgcgaacgac catcacctgc gccaggggga cggcgagcag
     2221 cccaccgcgt ccgggacggt ggcctgacat ggcccactcc gcggccacac cgcagccggc
     2281 ctacccgttg ccccgccccg ccaccgatgc ccggttcacc gtgggattga ccctcgacgt
     2341 cgccgccgtc ctgacccgcc acggctaccc gccgctgacc tgcggcggcg atctgctgcg
     2401 cctgcaacaa gccctgttca ccctcatcta ccaggagaac cggtgaccgt catcgacacc
     2461 gcactacacg ccagcctgcg caccctgaag ctgtccggca tgctggagac cctcgacgcc
     2521 cgcctcgctc aagcccaagc cggtcaactc
//
LOCUS       DATADN010000792         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_239119, whole genome shotgun sequence.
ACCESSION   DATADN010000792 DATADN010000000
VERSION     DATADN010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239119"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VHH53_10300"
     CDS             complement(<1..666)
                     /locus_tag="VHH53_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX2290559.1"
                     /translation="MMNPISRRRRWPIALAMSACLALSGCATDGLASLPLPAPGIGSG
                     GYRLTAVFSNALNLPANAKVKLAGADVGQMESMEARNYTAVTTLRIMDGVQVPQGSTA
                     ELRSATPLGDIFVAIKPPFPADPNAPLLTDGDTIPLESTMAAATVESVLSSAAVLVNG
                     GAVRNFTNIINGLGKATGDQGEAFGNLIRKTNGTLRTLNARSGQISTAITETSRLVEA
                     IEAK"
     gene            complement(663..1784)
                     /locus_tag="VHH53_10305"
     CDS             complement(663..1784)
                     /locus_tag="VHH53_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX2290560.1"
                     /translation="MRINKTKLLIAGAIALVVGLALATVAVIYTKDQLDTMTVTAQFD
                     SAAGLYEGNTVAVLGMPVGQVTKITPKGSYVEVDFTVDKDVKIPADAQAVTISNSILT
                     DRQIELTPPYSGSGPTLQDHATIGLHRTQTPVEFARVLDVLDKLSVSLKGDGKGNGPI
                     ADVVNASAAIADGNGQQMKDALGELSNALRLSADRGTVTRDQLTTIVRNLSSLSEAAA
                     RNDETLREFGSSVRALSQIMADENLGSGTTGKKINEVLTNTREVLETHRGAIKDLVAN
                     GDIVLNTAVDHERDLKEFLDVAPMTLDNLYNIVDQKNGALRVKVLTDRVLFDNQFIKE
                     VCNVMGLRQLGCSTGTLQDFGPDFGLTHMLDGLAAMGQR"
     gene            complement(1781..>2028)
                     /locus_tag="VHH53_10310"
     CDS             complement(1781..>2028)
                     /locus_tag="VHH53_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290561.1"
                     /translation="LEELLRNLRELSTLLARDGDGFLRSMLQSGPIALRGLANATGSG
                     NAAGFNASNGLLVDSWMCAISGRAKQFSMIEYFQDCK"
BASE COUNT          327 a          637 c          654 g          410 t
ORIGIN      
        1 tttggcctcg atggcctcga ccagccgcga ggtctcggtg atcgcggtcg agatctgccc
       61 ggaccgggcg ttgagcgtgc ggagggtgcc gttcgtcttg cggatgagat tgccgaatgc
      121 ctcgccctga tcgccggtcg ccttgcccag tccgttgatg atgttggtga aattgcgcac
      181 cgcaccgccg ttcaccaaca ccgcggccga gctcagcacc gattcgacgg tggctgcggc
      241 catcgtcgac tccaacggga tcgtgtcgcc atcggtcaac aagggcgcgt tcggatcggc
      301 ggggaacggc ggtttgatcg cgacgaaaat atcgccaagg ggtgtcgccg aacgcaattc
      361 ggcggtgctg ccctggggca cctggactcc gtccatgatg cgcaatgtgg tcaccgcggt
      421 gtagttgcgc gcctccatcg actccatctg cccgacgtcg gcaccggcca gcttcacctt
      481 ggcgttggcg ggcaggttca gcgcgttgga gaacaccgcc gtcagccggt agccgccgga
      541 cccgatgccg ggtgccggca gcggaaggct ggccaaaccg tcggtggcac agccggaaag
      601 ggccagacac gccgacatcg ccagcgctat cggccaacgg cggcgcctgc tgatcgggtt
      661 catcatctct gccccatcgc cgcgagtccg tccagcatgt gcgtcagccc gaagtccggc
      721 ccgaagtcct gcaacgtccc cgtgctgcag cccagttgcc gcagccccat cacgttgcac
      781 acctccttga tgaactggtt gtcgaacaac acccggtcgg tgagcacctt gactcgcagg
      841 gcgccgttct tctggtcgac gatgttgtac aggttgtcca gtgtcatcgg cgccacgtca
      901 aggaattcct tgagatcgcg ttcgtggtcg acagccgtgt tcagcacgat gtcgccgttg
      961 gcgacgaggt ccttgatggc gccgcgatgg gtttcgagta cctcgcgggt gttggtcaac
     1021 acctcgttga tcttcttgcc ggtggtaccg ctgccgaggt tctcatcggc catgatctga
     1081 ctgagtgccc gcaccgacga gccgaactcg cgcagcgtct cgtcatttct ggcggccgcc
     1141 tctgacagcg aacttaaatt ccggacgatg gtggtcagct gatccctggt caccgtgccc
     1201 cgatcggcgc tgagccgcag ggcattcgac agctcgccga gcgcgtcctt catctgctgg
     1261 ccgttgccgt ccgcgatcgc ggcgctggcg ttgaccacat cggcgatcgg gccgttaccc
     1321 tttccgtcac ccttcagaga taccgatagt ttgtcgagaa cgtcgagcac ccgggcgaat
     1381 tcgaccgggg tctgagtgcg gtgcaggccg atggttgcgt gatcctgcag ggtcggaccg
     1441 ctgcccgagt agggcggcgt cagttcgatc tgccggtcgg tcagaatcga gttggaaatt
     1501 gtcaccgcct gggcgtcggc gggtatcttg acatctttgt cgacggtgaa gtcgacctcg
     1561 acgtagctgc ccttgggggt gatcttggtg acctggccga ccggcatccc gagaaccgcg
     1621 acggtgtttc cctcgtatag acctgcggca ctgtcgaact gtgcggtcac ggtcatggtg
     1681 tcgagttggt ccttggtgta gatcaccgcg acggtggcca acgccagacc cacgaccagt
     1741 gcgatcgcac cggcaatcag cagcttggtc ttgttgatcc tcatttgcag tcctggaagt
     1801 actcgatcat gctgaattgc ttggcacggc cgctgatggc gcacatccac gagtcgacca
     1861 gcaggccgtt ggacgcgttg aacccagctg cattgccgct gccggtggcg ttggccaagc
     1921 cacgcagggc gattgggccg gactgcagca tgctgcgcag gaaaccgtcg ccgtcgcggg
     1981 cgagcagcgt ggacagctca cgcagattgc gcagcagttc ctcgagtt
//
LOCUS       DATADN010000793         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1292006, whole genome shotgun sequence.
ACCESSION   DATADN010000793 DATADN010000000
VERSION     DATADN010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292006"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..442
                     /locus_tag="VHH53_10315"
     CDS             221..442
                     /locus_tag="VHH53_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290562.1"
                     /translation="MADVAELQATGVGEDAGVGVEDPQLWTGTVGRDAAGVGEIAVAR
                     DPIWPRFPILANRHPFWHPVGNGSKERGG"
     gene            439..1269
                     /locus_tag="VHH53_10320"
     CDS             439..1269
                     /locus_tag="VHH53_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2290563.1"
                     /translation="MSDDPNAVVSHYGDGGLRQRIEQGLTAAGVDLRAVTTRDLAPVD
                     EFHSRGRLATRELAALAAPVSGERVLDVGSGLGGPARYLAETFGCEVTGIDLTPEFCE
                     VGNWLSELTGLSPKVRLETGSGTALPLPDGHFDLAWTMQMQMNISDKARLYREIHRVL
                     RPGGRFVLQELAQGAGGPCHLPAPWSSDPATSHLVSPDDLRGVVEAAGFVTTEWRDTT
                     DDALAWYAQHNSKVDAALPVLGTHLVMGDDHERKRHNVQRNYREGRLHQVMGAFRRGD
                     "
     gene            1418..2206
                     /locus_tag="VHH53_10325"
     CDS             1418..2206
                     /locus_tag="VHH53_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2290564.1"
                     /translation="MLSYPSAISLSSRSLTHLAELLRTHRRARRSRWRRLDPGRQALL
                     VLAHLRNGDTPARLAAGFSVGATTAWRYIREAVDLLAATAPTLAEAMVGIARLAYAIV
                     DGTLIRTDRLSGSADRRYYAGKHRHHGVNVQVIADPAGRLKWISPALPGSTHDLSAAR
                     EHTIIDALAEAQVMTFADKGYQGAGGTVRTPSKRHHRRPPLSRNQKEVNRAHARIRGI
                     GERAIATLKRWLLLAKLRCCPQRASALLAAILVLQLIEEQRTAS"
BASE COUNT          365 a          846 c          758 g          328 t
ORIGIN      
        1 caccgacgcc ttcaccgacg agtccgaaca ggtcggcctg ctgttcgcca cccacgccgc
       61 cgtggcgatg gccgacacac agagcccttt ccaacctgag ctggcggctc ggggtgtgtg
      121 cggcgtgtcg tccccacgtc caggttcgcc tcgacagcca cgactacctc catgatctcc
      181 ggtgcggacc ctccgcacgg cggggcccct ggtgctcgag gtggctgacg tagcggaact
      241 tcaggccacc ggcgtcggcg aagacgccgg cgtcggcgtg gaagacccgc agctgtggac
      301 gggcaccgtc gggcgcgatg cggcaggcgt tggcgaaatc gccgtcgctc gagatccaat
      361 ctggccgcgg tttcccatcc tcgcaaatcg gcacccgttc tggcaccctg tgggcaacgg
      421 atccaaggag cgcggcggat gagcgacgac ccgaacgcgg tggtgtccca ttacggcgac
      481 ggcgggctac gccagcgcat cgagcaaggc ctgaccgcgg ctggtgtcga cctgcgcgcg
      541 gtcaccaccc gcgacctggc tccggtggac gagttccact cccggggccg cctcgccacg
      601 cgcgagctgg cggcactggc ggcgccggtt tccggcgagc gggtgctgga cgtgggttcg
      661 ggcctcggcg gaccggcgcg ctatctggcc gagaccttcg gctgcgaggt caccggcatc
      721 gacctgacgc ccgagttctg cgaggtgggc aactggctga gcgagctcac cgggctctcg
      781 ccgaaggtgc gccttgaaac cggttccggc accgcgctgc cgctgcccga cggccacttc
      841 gacctggcct ggaccatgca gatgcagatg aacatcagcg acaaggcgcg cctgtaccgg
      901 gagatccacc gcgtgctgag acccggtggg cgcttcgtgc tgcaggagct ggcgcaaggt
      961 gcgggcggcc cgtgccacct gccggcgccg tggtcttccg atcccgccac cagccacctg
     1021 gtgagcccgg acgacttgcg cggtgtggtg gaggccgcgg gcttcgtcac caccgagtgg
     1081 cgcgacacca cagacgacgc gctcgcctgg tacgcgcagc acaacagcaa ggtggatgcg
     1141 gcactgccgg tgctcggcac ccacctggtg atgggcgatg accacgagcg caagcggcac
     1201 aacgtgcaac gcaactaccg cgagggacgc ctgcaccagg tgatgggggc gtttcgccgc
     1261 ggcgactgag ccgcgtcttc attgagcctt ttccaacctg ttgtggctgg tcgggtggcc
     1321 tgcggcgggt gaggcatcca gcggggtcga catggggaag ggctccccgg taagacagca
     1381 aacgaccaag aacgttgccc tcccagggag ccctccgatg ctgtcctacc cttcggcgat
     1441 cagcctgtcc agccgcagcc tgacccacct ggccgagctg ctgcgcaccc accgccgggc
     1501 ccgccgctcc cggtggcggc gcctggaccc cggccggcag gcgctgctcg tgctggccca
     1561 cctgcgaaac ggagacaccc cggcccggct ggccgccggg ttcagtgtcg gcgccaccac
     1621 cgcgtggcgt tacatccgcg aagccgtcga cctgctcgcc gccaccgcac ccaccctcgc
     1681 cgaagcgatg gtaggaatcg cgaggttggc gtacgcgatc gtcgacggca ccctgatccg
     1741 caccgaccgg ctgtccgggt ccgcggatcg gcgctactac gccggcaagc accgccacca
     1801 cggcgtcaac gtccaggtca tcgccgaccc ggctggccgc ctgaaatgga tctcacccgc
     1861 gttaccgggc tcgacgcacg acctgtccgc cgcccgcgag cacaccatca tcgacgctct
     1921 cgccgaggcg caggtgatga ccttcgccga caagggttac cagggcgccg gcggcaccgt
     1981 tcgcacgccg tccaaacgtc accaccgccg cccaccgctg tcgcgcaacc agaaggaggt
     2041 caaccgcgcc cacgcccgca tccgcggcat cggtgaacgg gccatcgcca ccctcaagcg
     2101 ctggctgctg ctggccaagc tgcgctgctg cccccaacgc gccagcgccc tgctcgccgc
     2161 gatcctcgtc cttcaactca tcgaggagca gcgcaccgca agttgaaaat ggctccctca
     2221 tgacgctcgg cagctaccgg gaatccgtcg ctcggcatcg gaccgaactg ctgaaagcac
     2281 acctcaggca agcgacg
//
LOCUS       DATADN010000794         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_701250, whole genome shotgun sequence.
ACCESSION   DATADN010000794 DATADN010000000
VERSION     DATADN010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701250"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1723
                     /locus_tag="VHH53_10330"
     CDS             386..1723
                     /locus_tag="VHH53_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2290565.1"
                     /translation="MEQEQREQTDTAEPALARRISRPVLLFLVLGIVLGTGIYTLVGS
                     VAARVGGLTWAAFVLALALAGISVPAYAELVTKYPRAAGAALYVRLAFDRPLLTFLVG
                     ACVLTSVVAGAATAARAFAGSYLAALVDVPVLPVVISFVLLAGVVALRGITESTRTAV
                     VLTVVELLGLLLVVAVGVAAVLAGTGDLSRATVLPAGASPLPALIGAAGLAVFLFTGF
                     DEANQFVEETRNPTRALPLALYGGLTVAAVVYVLVSLAAVTVVPATELSTTNAALVTV
                     VERGPLPLPSELFTVFGLVAVSNNGLLSLVVASRLLYGLGQQRTIPALFGRVGRRASP
                     TIAVLTTVALTVILAMSGAVEQLASTTVVALLAVFVVVNICCLRLRRDRVDHDHYRAP
                     TLLPVLGIGVSLALFTTQSVRSFLLAGLLMTVFIALYLLAQLGARRHPARNRT"
BASE COUNT          287 a          647 c          641 g          363 t
ORIGIN      
        1 cactcaccag cctcgtgcgt ttgaaactga gtgtgaacgt cagtgaacct gtgcgactgc
       61 ctcgatgagt acgacatcac cgagctgatc accacctacc gcgaaggcgc cagcgctgcc
      121 ttcctcgccg ctgcccacgg cgtgggcctc tgtccagcgc ctcctgcgca tcgctggtgt
      181 ccgccggatg ccacccactc gtcgatctat gaaaggcaat gccgggtgcg acgcatccgt
      241 agccgctagc ctgggcagag atccacatcc gtcagccgag cgcgacactg aaggcggagc
      301 gaatgttgcg gtgttggccc gaagtgcagt gggcaagtcg aggctgatct ggcgcgtgcc
      361 gtgtcctcag tgagtggggg gctgagtgga acaggagcag cgggaacaga cggacaccgc
      421 agagccggca ctggcccgtc gcatctcccg gcctgtgttg ctgttcctcg tcctggggat
      481 cgtgctcggc acgggcatct acacccttgt tgggagcgtg gccgcgcggg ttggggggtt
      541 gacctgggct gcgttcgtcc tcgcactcgc gctcgctggg atcagcgtcc cggcgtacgc
      601 cgagttggtg acgaagtacc ctcgagctgc gggagccgcg ctgtatgtcc ggctggcctt
      661 tgaccgtccg ctgctcacct tcctggtggg tgcctgtgtg ctgacctccg tggtggcggg
      721 tgcggccacg gcggcgcgcg cgttcgccgg tagttacctt gccgccctag tcgatgtgcc
      781 cgtgctgccg gtggtgatca gcttcgtgct gctcgccggt gtggtggcgc tgcggggcat
      841 cacggagtcc acccgcacgg ctgtcgtgct gaccgtcgtg gaactgctgg gtctgttgct
      901 cgtggtggcg gttggagtgg cggccgtgct cgccggcacc ggtgacctca gccgtgccac
      961 ggtcctccca gcgggtgcct caccgctgcc cgccctgatc ggtgccgccg gcctggcggt
     1021 gttcctgttc accggatttg atgaggccaa ccagttcgtc gaggagaccc ggaatccgac
     1081 gcgggcactc ccgctcgccc tctacggcgg gctcacggta gccgcggtcg tatatgtgct
     1141 ggtcagcctc gctgcggtga cggtggtgcc ggccaccgag ctgtcgacca ccaacgccgc
     1201 cctggtcacc gtggtcgagc gtggcccgct gccactaccc agtgagctgt tcaccgtctt
     1261 tggactcgtc gcggtaagca acaacgggct gctgagcctc gtcgtcgcaa gccggctgct
     1321 ctacgggctc gggcagcagc ggacaattcc ggccctgttc ggccgggtcg gccgccgcgc
     1381 cagccccacc atcgcggtgc tcacgaccgt ggcgctgacg gtgatactcg cgatgagcgg
     1441 cgcggttgag cagctcgcga gtaccaccgt cgtggcattg ctcgccgtgt tcgtggtggt
     1501 gaacatatgc tgcctacggc tgcgtcgcga ccgagtcgac cacgaccact accgagcacc
     1561 gacactgctg ccagtactcg gcatcggagt aagccttgcc ctgttcacca cacaaagcgt
     1621 ccgatcgttc ctgctcgccg ggctgctgat gacggtattc atcgcgctat acctcctcgc
     1681 ccagctcggc gcccgccgcc accctgcccg caaccggacg tgaaccattc actccacaac
     1741 caaatcacgt cgacggaaaa cgacattcga aggcgggtgt gcagtcgatg tccatggcca
     1801 cccccgcgct tcgagtgaag cggtgacgga atcatcggct atcaacgccc acacggtcgg
     1861 gctgggcgag cgggcgccag ttggctaagt tgacgtccat caggcgagcg aagggactcg
     1921 cacacggaga atgcccag
//
LOCUS       DATADN010000795         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_82606, whole genome shotgun sequence.
ACCESSION   DATADN010000795 DATADN010000000
VERSION     DATADN010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82606"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VHH53_10335"
     CDS             complement(<1..1456)
                     /locus_tag="VHH53_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX2290566.1"
                     /translation="MGEQRPGRRSRRDVLHLGTVLGVAGTAASFLPAIAFGAQSATGD
                     ETRTIRGRLEPGVPDWVYLPVNVPRGVREIEVVYRYDQPPTTPGNALDIGIFGPSGIH
                     RGFRGWSGGFRDRFAISASAATPGYLPGPIDSGTWHVILGPYTIAPQGMNYEIDVTLR
                     YGPPGRPFQPAPAPERASGRGRCWYRGDAHLHTVHSDGVRTPSELAAQARQARLDFVV
                     STEHNTPSASLIWGQHAGPDLLIVDGEGVTTRSGHWLALGLAVGEWIDWRYRAGQPAE
                     FRRFADRVHDRGGLVVAAHPYCPFTGCPWEFGYDRTDAIEVWNGPWGADDEASVRTWD
                     HLLRRGRWLPAVGNSDAHREPQVVGLPQTAVLAEELTTEAVLDGLRAGRCYLAESADQ
                     QLLMTATDQRATAGIGERLAVDPDQEVDVEVSATGAAGCTVHLLVWVHRQALPDPGGR
                     ALVGGCHRELLVGGLGEIAPPGPQPVEHRLSGELL"
     gene            1540..>1916
                     /gene="rnhA"
                     /locus_tag="VHH53_10340"
     CDS             1540..>1916
                     /gene="rnhA"
                     /locus_tag="VHH53_10340"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX2290567.1"
                     /translation="MADQAAERVVDIYTDGACSGNPGPGGWGAVLRYGTHEKEIYGGQ
                     SAETTNNRMELMAPIQALESLTRPVVVRLHTDSKYVLDGVTKWLPRWKSNGWQTAGKQ
                     PVKNADLWRRLDAALHRHQVQWR"
BASE COUNT          313 a          662 c          678 g          263 t
ORIGIN      
        1 cgaggagctc accactgagg cggtgctcga cgggttgcgg gccgggcggt gctatctcgc
       61 cgagtccgcc gaccaacaac tctcgatgac agccaccgac cagcgcgcga ccgccgggat
      121 cgggcagcgc ctggcggtgg acccagacca ggaggtgcac ggtgcagcca gccgctccgg
      181 tcgcgctcac ctcgacgtcc acctcctggt ctgggtccac cgccaggcgc tccccgatcc
      241 cggcggtcgc gcgctggtcg gtggctgtca tcaagagttg ctggtcggcg gactcggcga
      301 gatagcaccg cccggcccgc aacccgtcga gcaccgcctc agtggtgagc tcctcggcga
      361 gcacggcggt ctgcggcagc ccgaccacct gaggctcgcg gtgtgcatcg gagttgccga
      421 ccgcgggcag ccaccggccg cgacgcagca ggtggtccca ggttcgcacg gacgcctcgt
      481 cgtcggcgcc ccacgggccg ttccacacct cgatcgcgtc ggtgcggtcg tagccgaact
      541 cccatgggca tccggtgaac gggcagtatg ggtgcgcggc gacgacgagc ccaccgcggt
      601 cgtgcacccg gtcggcgaac cggcggaact cagccggctg cccggcgcgg taacgccaat
      661 cgatccactc ccccaccgca aggcccagcg ccagccagtg cccggagcgc gtggtgaccc
      721 cctcaccgtc cacgatgagc aggtcggggc cggcgtgctg gccccagatc agcgaggcag
      781 acggggtgtt gtgctcggtg gagacgacaa agtcaagccg cgcctgccgg gcctgcgcgg
      841 ccagttcgga aggggtacgt acaccatcgg agtgcacggt gtgcagatgg gcgtcgccgc
      901 gataccagca gcggccgcgg ccgcttgccc gctcgggcgc cggtgccggc tggaacggcc
      961 ggccgggcgg gccgtaacgc agtgtcacgt cgatctcgta gttcatgccc tgcggggcga
     1021 tggtgtacgg gccgaggatc acgtgccagg taccggaatc gatcgggccc ggcaggtaac
     1081 cgggggtggc cgcgctcgcc gagatcgcga accggtcccg gaacccacca gaccagcccc
     1141 ggaagccccg gtgaataccc gaggggccga agattccgat gtccagcgcg ttgccagggg
     1201 tggtcggggg ctggtcgtag cggtagacca cctcgatctc ccgcaccccc cgcggaacgt
     1261 tgaccggaag gtagacccag tctggcacgc cgggctcgag ccgaccgcgg atcgtacgcg
     1321 tctcgtcgcc ggtggccgac tgcgccccga aggcgatggc cggcagaaag ctcgccgcgg
     1381 ttcccgccac acccagcacg gtccccaggt gcagcacatc gcgccggctt cgccgacccg
     1441 gccgttgttc acccacaccg cgacgttagg cgatccacga taaccacggc gatactcgtc
     1501 aggtccttcg cggagagctg tggtcggtat gttgccggtg tggcagacca ggcagccgaa
     1561 cgggtggtgg acatttacac cgacggtgcc tgtagcggca atcccgggcc gggcggatgg
     1621 ggtgccgttc tgaggtacgg cacgcacgag aaggagatct acggaggcca gtctgccgag
     1681 accaccaaca accggatgga gctgatggcc ccgatccagg cgttggaaag cttgaccagg
     1741 cctgtggtgg tgcggctgca caccgatagc aagtacgtgc tggacggcgt caccaagtgg
     1801 ctgcctcgat ggaagagcaa cggctggcag accgccggca agcaacctgt caagaacgcc
     1861 gacctgtggc ggcgcctcga cgccgccctg caccggcatc aggtgcagtg gcgctg
//
LOCUS       DATADN010000796         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1292233, whole genome shotgun sequence.
ACCESSION   DATADN010000796 DATADN010000000
VERSION     DATADN010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292233"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VHH53_10345"
     CDS             complement(<1..193)
                     /locus_tag="VHH53_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290568.1"
                     /translation="MWHGNVYKALQVVQSIEMDLDESPLARLVTPPLTAETTPLAVLS
                     HPPLTEDQLPLAMLPNVPAP"
     gene            477..1655
                     /locus_tag="VHH53_10350"
     CDS             477..1655
                     /locus_tag="VHH53_10350"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX2290569.1"
                     /translation="MVLPLDGVRVVSLGVSISGPLGLRHLADFGAEVIKVEPPGGDMV
                     RHWDSAVGGEGSAHFWVNPNKRSVVLNLKDERGRAVLRKLIAEADVFLENFSPGTAGR
                     LGFGYEEVRRIRPDIIYIHASGYGQDGPYRDMKAFDGLIQAETGVMEMTGTPESPAKV
                     ALSICDGVTALFVALSVMTALRQRDRTGEGQEVDVSMFDCMTSMLGYFPFRHFYEGYV
                     PQRVGTGHHLLAPYGAYPAKGDKLIAVACASEATWHRFAEALGHPEWKDDPRFRDNAS
                     RYAHRAALEAAFLPILAAREREEWRQVFLDQGVPCASVNSLAEVCDHPQAQHRELFPE
                     VEGPYGHIRLCRFPVRFSGLQPRLAHPPKTGADTAAVLRELGIDDTEIAALRQEGVIG
                     "
     gene            1688..2839
                     /locus_tag="VHH53_10355"
     CDS             1688..2839
                     /locus_tag="VHH53_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2290570.1"
                     /translation="MDFQFDDVQQSIRRMVKAFVDREVRPVAMAYELEDRYPTPIVER
                     MRELGFFGFTIPEQYGGSGLDEVCYAILMEELSVGWMSLAGILGTHMMTAWMILHYGT
                     AEQRDAYLPKMATGEWHAGMALTEPGSGSDAAALRTAARREQDQWVVSGTKMFVSNAE
                     HATMFSVFVRTDPQRPKAKGISCLLIHKGTPGFRVGRHLKKLGYRGLRTSELIFEDAR
                     VPVGNLLGKENEGFPMIMSALEGGRINVAARSVGLARAAFEDSIRYAQQRETFGKPIA
                     QHQLIQAKLAEMATRIEAAKLLTYQAASLKAAGRRCDLEAGMAKFFASETAEFCASEA
                     IQIHGGMGYIQELPVERYFRDSKLLTVGEGPNEIQRLVIARRLLERFPV"
     gene            complement(2802..4244)
                     /locus_tag="VHH53_10360"
     CDS             complement(2802..4244)
                     /locus_tag="VHH53_10360"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX2290571.1"
                     /translation="MTHHATRQLAEFVASTSLSHMPDEVIDRAKYFVLDYLGVALRGS
                     LVDSSAAVRAFADRFAPPGHATIIGGDQGTHPAYAALANGAAAHCLEMDDTHQEGSIH
                     LGAPVISALMAASEMQPASGGEFLTAVVLGYEVAARLAMAVGPEAHYRRGFHPTGTCG
                     TFGAAAAVARLWGLEARAIAQALGIAGSQAAGSMEFLATGAWTKRFHPGWAAHNGLIA
                     AALAREGFTGPETIIEGQAGFAQAYSQAPRPERVTQGLGTSFEILATAVKPHACCRYM
                     QAPIDAMLDLVRTHHIAADDVAQVTLGILDTAFPIICEPAAIKYAPRSVVDAQFSMPF
                     GAAVALLCRRASLAEFTPEMVTAPRVRALMQRVAHARDPELERHFPREWPAWASIALH
                     DGRKVSITVRYPKGDPQNPLSWDELLAKYRELGRSVLPEDRLDELAPRVRALESAPDV
                     APLWCLLRPTRASAAQDSLTPGNVPAAGGR"
     gene            complement(4964..>5430)
                     /locus_tag="VHH53_10365"
     CDS             complement(4964..>5430)
                     /locus_tag="VHH53_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX2290572.1"
                     /translation="VLRQHPHVEATLFDVPAVAALARQRLASSPVASRLRIVEGDFFT
                     DPLPEDHDAILLANVLHNFLPQRNRALLARVHACTPAGARLLLVDFWTDPTHTQPLTA
                     ALMAGAFLLVTGEGDVYSVEDVRDWLQATGWRLLAHHPLAGPTSMIVAEKAA"
BASE COUNT          956 a         1791 c         1825 g          858 t
ORIGIN      
        1 caggagcggg gacgttcggc aacatcgcca gcggcagctg gtcctcggtc agcgggggat
       61 gggacaacac ggccagcggc gtggtctctg ccgtcagcgg gggagttacc aatcgggcca
      121 gcggcgactc atcgaggtcc atctcgatgg actggaccac ttgcaaggcc ttgtagacat
      181 ttccatgcca cacgtaccat actctccgtg gccgctccga gatgagtctg agcccccctc
      241 atgcgccttg tcgagtgagt gtcaaaaaaa cccccgaact ggtatcatag gggccggcat
      301 gatcccgccc acgggcaggc gtgacatgcg cttggggcgc gacagtgcat gaccgcaggt
      361 gccagcggcc tctgctggct tcaggccgcc aatggtccaa ggggcctgcg gggccctgac
      421 cagtgtggtg gcgttcttat ttcgcgggaa aaacccgggg gatgttgagg tgttatatgg
      481 tgcttcctct cgatggggtg cgcgttgtct cgctgggggt gtcgatcagc ggtccgctgg
      541 ggctccgcca cctggccgac ttcggtgcgg aggtcatcaa ggtcgagccg ccgggcggcg
      601 acatggtgcg ccactgggac agcgcggtgg gcggtgaggg atcggcccat ttctgggtga
      661 acccgaataa gcgaagcgtg gtgctcaacc tcaaagacga gcgcggccgc gcagtcctcc
      721 gcaagctcat cgccgaggcg gacgtcttcc tggagaactt tagccccggc acggccgggc
      781 gcctgggctt cggctacgag gaggtgcggc gtatccgccc ggacatcatc tatatccacg
      841 cctcgggcta cgggcaggac ggcccctacc gcgacatgaa ggccttcgac gggttgatcc
      901 aggccgaaac gggggtgatg gagatgacgg gcacgcccga gtcgcccgcc aaggtggcgc
      961 tgtccatctg cgatggcgtg acggcgctgt tcgttgccct gagcgtgatg acggccctgc
     1021 gccagcgcga ccgcacgggc gagggccagg aggtcgacgt ctccatgttc gactgcatga
     1081 cgagcatgct cggctacttt cccttccggc acttctacga gggctacgtc ccccagcgcg
     1141 tgggcacggg gcatcacctg ctcgccccct acggcgccta cccggcgaag ggcgacaagc
     1201 tcatcgccgt ggcctgcgcg agcgaggcga cgtggcaccg cttcgccgag gcgctgggcc
     1261 accccgagtg gaaggacgat ccgcgcttcc gcgacaacgc ctcccggtac gcgcacagag
     1321 cggccctgga ggcggcgttt ctgccgatcc ttgccgcgcg ggagcgcgag gagtggcggc
     1381 aggtgtttct ggaccagggc gtgccctgtg cgagcgtgaa ttcgctggcc gaggtgtgcg
     1441 atcatcccca ggcgcagcac cgtgagctgt tccccgaggt cgaggggccc tacggccaca
     1501 tccgcctgtg tcgcttcccg gtgcgcttca gcgggctgca gccacgcctg gcgcacccgc
     1561 ccaagacggg cgcggatacg gcggccgtgc tgcgtgagct agggatagac gacacggaga
     1621 tcgccgccct acggcaggag ggtgtcatcg gctagcggcg ggcggtcgcc cgcctgggag
     1681 gagcgccgtg gatttccagt tcgacgacgt ccagcaaagc atccgccgga tggtgaaagc
     1741 gttcgtggac cgggaggtcc gacctgtcgc catggcgtac gagctggagg accgctatcc
     1801 cacgcccatt gtcgagcgga tgcgggagct gggctttttc ggcttcacca tcccggagca
     1861 gtacggtggc agcgggctgg acgaggtgtg ctacgccatc ctgatggagg agctctcggt
     1921 cgggtggatg agcctcgccg gcatcctcgg cacccacatg atgacggcgt ggatgatcct
     1981 gcactacggc accgccgagc agcgcgacgc ctacctcccg aaaatggcca ccggcgagtg
     2041 gcacgcgggc atggcgctca ccgagccagg ctcgggctcg gacgccgcgg cgctgcgcac
     2101 ggcggcgcgc cgggagcagg accagtgggt ggtcagcggc acaaaaatgt tcgtctccaa
     2161 cgccgagcat gccaccatgt tcagcgtctt cgtgcgcacc gacccgcagc ggcccaaggc
     2221 gaagggcatc tcctgcctcc ttatccacaa agggacgccg ggcttccggg tcggccgcca
     2281 tctgaagaag ctgggctacc gtgggctgcg caccagcgaa ctcatctttg aggacgcgcg
     2341 cgtgccggtg gggaacctgc tcggcaagga gaacgagggc ttcccgatga tcatgagcgc
     2401 cctggagggc gggcgcatta acgtggcggc gcgcagcgtg ggcctcgccc gggcggcctt
     2461 tgaggactcg atccgctacg cccagcagcg cgagaccttc ggcaagccca ttgcgcagca
     2521 ccagctcatc caggccaagc tcgccgagat ggccacgcgc atcgaggccg ccaagctcct
     2581 gacctaccaa gcggcctccc tcaaggcggc gggccggcgg tgcgacctcg aggcggggat
     2641 ggccaagttc ttcgcctccg agaccgccga gttctgcgcc agcgaggcca ttcagatcca
     2701 cggcggcatg ggttacatcc aagagctgcc cgtggagcgc tacttccgcg actccaagct
     2761 cctcaccgtg ggcgagggcc cgaacgagat ccagcggctc gtcatcgccc gccggctgct
     2821 ggaacgtttc ccggtgtaag cgagtcctgg gcagcggatg ccctggtggg acggagcaga
     2881 caccagagcg gcgccacgtc cggggcggac tccaaagctc gcacgcgcgg ggcaagctcg
     2941 tccagccggt cctccggcag cacactgcgc cccagctccc ggtatttggc cagcaactcg
     3001 tcccacgaca ggggattctg cggatcgccc ttgggatacc ggacggtgat ggacaccttg
     3061 cggccatcgt gcagcgcgat gctggcccag gccggccatt cgcgcggaaa gtgccgctcc
     3121 agctccggat cgcgggcatg cgcgacgcgc tgcatcagcg cacgcacgcg cggggcggtg
     3181 accatttccg gggtaaactc cgccagcgag gcgcgccggc acagcagcgc caccgccgcg
     3241 ccaaacggca tgctgaactg cgcatcgacc accgaccgcg gcgcgtattt gatggcggcg
     3301 ggttcgcaga taatcggaaa cgccgtgtcc aggatgccca gcgtcacctg cgccacgtcg
     3361 tcggccgcga tatggtgcgt cctcaccagg tccagcatgg catcgatcgg cgcctgcata
     3421 taccgacagc aggcgtgcgg tttgaccgcc gtcgccagga tttcaaacga cgtgcccaag
     3481 ccctgcgtca cccgctcggg gcgcggcgcc tgtgagtagg cctgggcaaa gccggcctgg
     3541 ccctcgatga tggtctcagg gccggtaaaa ccctcgcgtg ccagcgctgc ggcgatcaac
     3601 ccgttgtgcg ccgcccagcc ggggtggaag cgtttggtcc aggcgcccgt cgccaaaaat
     3661 tccatggacc ccgcggcctg gctgccggcg atgccgagcg cctgcgcgat cgccctcgcc
     3721 tccaggcccc acaaccgcgc cacggccgcg gcggcgccaa acgtgccgca cgtgccggtc
     3781 ggatgaaagc cgcgccggta gtgcgcctcg gggccaaccg ccatggccaa ccgcgccgcg
     3841 acttcgtagc ccagcaccac ggcggtgaga aactctccgc cgctcgctgg ctgcatctcc
     3901 gaggcggcca tgagggcgga gatcaccgga gcgcccaggt ggatggagcc ctcctggtgc
     3961 gtatcgtcca tctcaagaca gtgcgctgcg gcgccgttgg cgagcgcggc ataggccgga
     4021 tgggtgcctt ggtcgccgcc gatgatcgtg gcatggccgg gtggggcaaa tcggtccgca
     4081 aaggcccgca cggcggcgct tgaatcgacc agcgagcccc gcagcgccac accaagatag
     4141 tcgagcacga agtactttgc ccggtcaata acctcgtcgg gcatgtgcga tagggaagtc
     4201 gaggccacaa actctgcgag ttgtcgcgtg gcatggtgtg tcatcgtcac cctccgctcc
     4261 ctgacaatag cgtaacatgt gacgccatga cccttctcca cacaagttta acacgtctct
     4321 attggccgtg tatttgaggg ccgaatgacg cccgatgcgc ctgtcatagt cttttccagg
     4381 tagtgctaca caggtaatgc tcatgccgca attccatgct actgttggtc aaaaacagcc
     4441 ccagaagtag ggaggtcaac agcagcgatg gtcgtcagag aggcgtgccc agcatgtgga
     4501 tcgaaccggt acaagaaaca tggccatact cgccatggca aacagcatca tcactgcaaa
     4561 gcgtgcgagc gccagtgtgt ggccagggct gaagaccgcg gcattgcgga tgagcaacgc
     4621 acccttattg gaccagcggg cgcaggcgaa aggcgccccc cgccagctca cggtccggct
     4681 gcaaacccat cacgaggcgc tccagaccgc gcggcaacgc caggagacgc cggagttcaa
     4741 agcccgatac gccctgcgag caggggtcga gagtagcctc tcgcaaggca cgagacgcat
     4801 ggttgactta cccaacagag tcaggacatc ctatacctcc gtgcgcacgg aagccgatcg
     4861 tattgtccca ctctgagcat gatgagctct ttgttaggtt tcgcagccca acgccaggca
     4921 tgaggctcgg ctccatgccc gtatgaagcg aggtggtcgc ttcttaggct gccttctcag
     4981 ccacaatcat gctagtggga cccgccagcg ggtggtgtgc cagcagccgc cagccggtgg
     5041 cctgcagcca gtcacgcacg tcctcaacgc tgtacacatc cccttcaccc gtcacgagca
     5101 gaaacgcccc cgccatgagc gcggccgtaa gcggctgcgt atgcgtcggg tcggtccaaa
     5161 aatcgaccag caacaggcgg gcccctgccg gcgtgcaggc gtggacccgc gccaacaagg
     5221 cgcggttgcg ctgcggcaaa aaattatgca acacgttggc gaggagaatg gcgtcgtggt
     5281 cctccgggag cgggtctgta aagaagtcgc cttccacgat gcgcagccgg gacgctacgg
     5341 gactgctagc caaccgctgg cgcgccagcg cggcgacggc gggcacgtcg aacagcgtcg
     5401 cctcgacgtg tgggtgctgg cgcagcacgg
//
LOCUS       DATADN010000797         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_239452, whole genome shotgun sequence.
ACCESSION   DATADN010000797 DATADN010000000
VERSION     DATADN010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239452"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VHH53_10370"
     CDS             complement(<1..494)
                     /locus_tag="VHH53_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2290573.1"
                     /translation="MTSSKPQVVVVTGASGGIGRASAQAFGARGAKVALLARGELGLQ
                     GAAKEVEAAGGTALPIPVDTADYEQIARAADRVELELGPIDVWVNAAFTSVFAPFIQI
                     KPQEYKRVTEVSYLGYVYATRVALDRMLPRDQGTIVQVGSALAYRGIPLQSAYCGAKH
                     AIQG"
     gene            complement(645..2438)
                     /locus_tag="VHH53_10375"
     CDS             complement(645..2438)
                     /locus_tag="VHH53_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX2290574.1"
                     /translation="MATQTVADYLLSRLREWGVQHVFGYPGDGINGLLAAWGRADNRP
                     QFVQARHEEMAAFEAVGYAKFTGRLGVCVATSGPGAVHLLNGLYDAKLDHVPVVAIVG
                     QTNRSAIGGSYQQEVDLLSLYKDVASDYVQMATVAEQLPNVLDRAIRTAVARRAPTAV
                     IIPADVQELEYTPPPHEFKMVPSSLGVDWSTPHPDEEAVRRAADVLNAGSRVAILAGS
                     GARGARAELAEIAELLGAGVAKALLGKDVLSDELPYVTGSIGLLGTRPSYEMMRGCDT
                     LLTVGSSFPYTQFMPEFGQARAVQIDIDPALIGMRYPYEVNLVGDAAATLRALIPLLD
                     RKPDRSWRESIESDVARWWETMAMQADLEADPINPMRLFAELSPRLPEDAILTADSGS
                     AANWYARQLRFRGSMRGSLSGTLATMGPGVPYGIGAKFGYPHRPVIVFAGDGAMQMNG
                     LAELITIKRYWAEWEDPRLVVAVLHNDDLNQVTWEMRALGGAPKFVQSQSLPDVDYAG
                     FAAGLGLQAIAVDKPEDVGPAWDRALGADRPTVLDVRCDPNVPPVPPHATYEQMKDAA
                     QALMHGDEDRWGVIKQGVKTKLQEFLPHREG"
     gene            complement(2636..>3338)
                     /locus_tag="VHH53_10380"
     CDS             complement(2636..>3338)
                     /locus_tag="VHH53_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290575.1"
                     /translation="LGLADGTVVALGERECSVQRRNQKLAEETPSPALTAGQRERLLA
                     AAVRAGEAVGYRNAGTVEFLFAPESGEFFFLEMNTRLQVEHPVTELRYGVDLVAAQLR
                     IAAGASPGFDVDALAPGGHVIELRINAEDPKRFLPGPGPVTEWVEPIGDGVRVDAGYA
                     KGTTVTPLYDSLLAKLVVHGRDRDQDLQRARAAVAGFTVAGPKCNLPFQAELLEHPEF
                     VSGRYDTGLVARMRS"
BASE COUNT          523 a         1221 c         1080 g          514 t
ORIGIN      
        1 aagccctgga tcgcgtgttt ggctccgcag taggccgact gcagcggaat gccacggtag
       61 gccagcgccg acccgacttg cacgatcgtg ccttggtctc gcggcagcat ccggtccaac
      121 gcgacgcggg tggcatagac atagccgaga taggacacct cggtgacccg cttgtactcc
      181 tgcggcttga tctgtatgaa cggcgcgaac accgaggtga aggctgcatt gacccacacg
      241 tcgatcggcc ccagctccag ctcgacccgg tcggcggctc gcgcaatctg ctcgtagtct
      301 gcggtgtcga cggggatcgg cagcgctgtc ccacccgcgg cttcgacctc cttggccgcg
      361 ccctgcaggc ccaactcgcc gcgggccagc aacgccacct tggcaccgcg ggccccgaag
      421 gcctgcgcac tcgcccggcc gatgccgccg ctggcaccgg tcaccaccac cacctgaggc
      481 ttgctactgg tcatcagtgc tcacctttct gcacgcaatg tccggtgcac cgcgccgacg
      541 tcgacgccgc gggccaggtc ggccaactgc ccagcgactc gaccgctatc cccgagccca
      601 ctcggcggtc ggcgtggcgg gtctcggtga caacccggca cggctcagcc ttcccggtgc
      661 ggcaggaatt cctggagctt ggtcttgaca ccttgcttga tcacgcccca ccggtcctcg
      721 tcgccgtgca tcagagcctg tgcggcatcc ttcatctgct cgtacgtggc atgcggcggc
      781 accggcggca cattggggtc acagcgcaca tccagcacgg tgggccggtc ggcgcccagc
      841 gcgcggtccc aggctgggcc aacgtcctca ggcttgtcga cggcgatggc ctgcaaaccc
      901 aaccccgcgg cgaatcccgc atagtccacg tcgggcaggc tctgggactg cacgaacttg
      961 ggcgccccgc ccagcgcgcg catctcccag gtgacctggt tgaggtcgtc gttgtgcaac
     1021 acggcgacca ccagccgggg atcctcccac tcggcccagt accgcttgat ggtgatcagc
     1081 tcggcgagac cgttcatctg catggcgccg tcgcctgcga acacgatcac cggccggtgc
     1141 gggtagccga acttggcccc gatgccgtag ggaacaccgg gtcccatggt cgccagggtg
     1201 cccgacagcg acccgcgcat cgagccgcga aagcggagct gccgggcgta ccagttggcc
     1261 gccgagccgg agtcggcggt gaggatcgcg tcctcgggta gccggggaga cagctcggcg
     1321 aacagccgca tcgggttgat cgggtcggcc tcaaggtcgg cctgcatcgc catggtctcc
     1381 caccagcggg caacgtcgga ctcgatgctc tcgcgccacg accggtccgg tttgcggtcc
     1441 agcaacggga tcagcgcgcg cagcgtggcc gccgcatcgc ccacgagatt gacctcgtag
     1501 gggtagcgca tcccgatcag ggcagggtcg atgtcgattt gcaccgccct ggcctggccg
     1561 aactcgggca tgaactgggt gtaggggaag ctcgagccga cggtcagcag ggtgtcgcag
     1621 ccccgcatca tctcgtagct cggcctcgtt ccgagcaggc cgatcgaccc ggtgacgtag
     1681 ggcagctcgt cggaaagcac gtccttgccc agcagcgcct tggctacccc ggcgcccagc
     1741 agctcggcaa tctccgccag ctcggcacgc gccccgcgag cgccggagcc ggcgaggatc
     1801 gccacccggc tgcccgcatt gagcacgtcg gccgcccggc ggaccgcctc ttcgtccggg
     1861 tgaggggtgg accagtccac cccgaggctc gagggcacca tcttgaactc gtggggcggc
     1921 ggtgtgtact ccagctcctg cacgtcggcc gggatgatca ccgcggtcgg cgcgcgccga
     1981 gccaccgcgg tgcggatcgc acggtcgagc acgttgggca gctgctcggc aaccgtggcc
     2041 atctgcacgt agtcgctcgc gacgtccttg tacaagctca gcaggtcgac ttcctgctgg
     2101 taagaaccac ctatcgcgct gcggttggtc tgcccgacga tcgccaccac gggcacgtgg
     2161 tcgagcttgg cgtcgtagag cccgttgagc aggtggactg cgccaggacc agaggtcgcc
     2221 acgcacaccc cgagccgccc ggtgaacttc gcgtagccga ccgcctcgaa agccgccatc
     2281 tcctcgtgcc gggcctgcac gaactgcggc cggttgtcgg cccgtcccca cgcggccagc
     2341 aacccgttga tgccgtcgcc ggggtaaccg aagacatgct gcacgcccca ctcccgcagc
     2401 cggctcagca ggtagtcggc caccgtctgt gttgccatgg gtcatcgctc ctcgacgtcc
     2461 gcggacgctc gtcgagcagg aatacccgca aagttgcatt tacgcacaga atggcggaaa
     2521 ggtctcagcg gcggttcatg ggcttacgcc tagcctgacc ggtgcgctgt gcacgtcgtg
     2581 gcttttcgac atgccagcgc gacacaccgt gcaccagcgc cgcacacgcg gggggtcagg
     2641 agcgcatccg ggcaacgagc ccggtgtcgt accgcccaga gacaaactcg gggtgctcca
     2701 gcagctcggc ttggaagggc aagttgcact tcggcccggc cacggtgaac ccggccaccg
     2761 ccgcccgcgc ccgctgcagg tcctggtcgc ggtcgcgacc gtgtaccacc agcttggcca
     2821 gcaaggagtc atacagcggc gtcaccgtgg tgcccttcgc atagccggcg tccacccgca
     2881 ccccgtcccc gattggctcc acccactcag tgaccggccc aggaccaggc aggaaccgct
     2941 tcgggtcctc ggcgttgatc cgcagttcga tcacgtgccc accgggtgca agagcgtcga
     3001 cgtcgaagcc gggtgacgcg cccgcagcga tgcgcagctg ggctgccacc aggtccacgc
     3061 catagcgcag ctcggtgacc gggtgctcca cctgcagccg ggtgttcatc tccaggaaga
     3121 agaactcccc gctctcgggc gcgaacagga actccaccgt gcccgcgttg cggtaaccga
     3181 ccgcctcgcc ggcccgcacc gccgcggcca gcaaccgctc acgctggccc gcggtcagcg
     3241 ccggtgacgg ggtctcctcg gcgagcttct ggttgcgccg ctgcaccgaa cactcccgct
     3301 cgcccagcgc caccaccgtc ccgtcggcca accccagg
//
LOCUS       DATADN010000798         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_82983, whole genome shotgun sequence.
ACCESSION   DATADN010000798 DATADN010000000
VERSION     DATADN010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82983"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VHH53_10385"
     CDS             <1..707
                     /locus_tag="VHH53_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2290576.1"
                     /translation="AVTACDSDESVHAIVITGAAPAFCAGADLDALAAAGRSGSVTDL
                     HRIYAGFLAVASAAVPTIAAVNGPAVGAGLNLALACDLRLAGPGASFDARFLQLGIHP
                     GGGMTWLVQRAAGPQTAAAMALFGEPLDAQEAVRTGLAYRLVEVGGNDPRSPAAHEVV
                     VAAAVDLARRTAAAPRDLVVATKAALRATAVMPLHADAVAYETGPQLASLRSELTRRC
                     HSEPNDGLGESAGAGG"
     gene            complement(687..>1501)
                     /locus_tag="VHH53_10390"
     CDS             complement(687..>1501)
                     /locus_tag="VHH53_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2290577.1"
                     /translation="LLAGQFGTDQGRTQDVVRAAAVVELVHLATLYHDDVMDEATLRR
                     GAVSANARWDNTVAILTGDFLFAHASRLVADLGPEAVRIIACTFAELVTGQMRETTGP
                     LPGTDPVAHYLAVVAEKTGSLIATAGRYGAMFSGASQERIDALRRFGEKIGTAFQISD
                     DLIDIASPAAQSGKTPGTDLREGVRTLPMLYALAEPGVDRLRALLAGPLTDETLIAEA
                     LHLLRSSPGLDRAVQSLRSYTDSARAELAVLPPGPAREALASVSDYLAARTG"
BASE COUNT          217 a          525 c          529 g          230 t
ORIGIN      
        1 gtgccgtcac ggcatgcgac agcgacgaat cagtgcacgc catcgtgatc accggcgctg
       61 cgcccgcctt ctgcgccggt gcggacctcg acgccctcgc cgccgccggg aggtccggct
      121 cggtcaccga cttgcaccgc atctacgccg gcttcctcgc tgtggccagc gccgcggtgc
      181 ccaccatcgc cgccgtcaac gggcccgccg tgggtgccgg actcaacctg gcgctggcct
      241 gcgatctgcg cctggccggc cccggcgcat ccttcgacgc ccgtttcctg caactgggca
      301 tccatcccgg tgggggcatg acgtggttgg tgcaacgcgc cgccggaccc cagaccgccg
      361 ccgcgatggc gctgttcggt gaaccgctgg acgcccagga ggcagtgcgc accggactgg
      421 cctaccgcct ggtcgaggtg ggcggcaatg atccccgctc ccccgccgcc cacgaggtag
      481 tggtggccgc tgccgttgat ctcgcgcgcc gtactgccgc agctccccgc gacctggtgg
      541 tcgctaccaa ggctgcgcta cgcgccaccg ccgttatgcc gctacacgcc gacgcagtcg
      601 cctacgaaac gggaccccaa ctagcctcac tccgttccga gctgacgcgg aggtgccatt
      661 cggagcccaa tgacggtttg ggtgagtcag ccggtgcggg cggctaggta gtcggatact
      721 gaggctaagg cttctcgggc ggggccgggg ggcagcaccg cgagttcggc gcgtgcggag
      781 tcggtgtagg agcgcaggga ctgcaccgcc ctatcgaggc cgggcgagct acgcagcagg
      841 tgcaacgcct cggcgatcag ggtttcatcg gtgagcgggc cggccagtaa cgcgcggagc
      901 cggtcgaccc cgggttcggc cagcgcgtag agcatcggca gggtgcgcac accctcacgc
      961 aggtcggtgc ccggtgtctt gccggactgg gcggccggcg aggcgatgtc gatgaggtca
     1021 tcggagatct ggaacgcggt gccgatcttc tcgccgaacc ggcgcagcgc gtcgatacgc
     1081 tcctgggaag cgccggagaa catcgcgcca tagcggccgg cagtagcgat gagcgatccg
     1141 gtcttctccg cgaccactgc caggtagtgc gccaccgggt cggtgccggg gagaggcccc
     1201 gtggtctcgc gcatctgacc ggtcaccagc tcggcgaagg tgcaggcaat gatccgcacc
     1261 gcttccggcc cgaggtcggc gaccaaccgg gaggcgtgtg cgaacaggaa atcgcctgtg
     1321 aggatcgcga ctgtgttgtc ccatcgggcg ttggcgctca ctgccccgcg ccggagggtg
     1381 gcctcgtcca tcacgtcgtc gtggtagagc gtggcgagat gcaccagctc caccaccgcg
     1441 gccgcgcgga ccacgtcctg cgttcgcccc tggtcagttc cgaactgacc agcgagcagg
     1501 g
//
LOCUS       DATADN010000799         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1161649, whole genome shotgun sequence.
ACCESSION   DATADN010000799 DATADN010000000
VERSION     DATADN010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161649"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VHH53_10395"
     CDS             complement(<1..513)
                     /locus_tag="VHH53_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore staphylobactin biosynthesis protein
                     SbnC"
                     /protein_id="HEX2290578.1"
                     /translation="MRDLVDTLIQENLWGFADAAQRCRPAALGTETVEADEWWCRIDV
                     ASGWVCFRARIAGALQPCRFSRPPVWHGDGSGSPPRQLRPDELLALLAGDCSDSSHVR
                     QGGAGLRTAVQHATVVRARRARLADARPRPGELLTAERLAATRNRPFHPTAHATVGWT
                     SAELARYGPLR"
     gene            complement(577..1572)
                     /gene="sbnA"
                     /locus_tag="VHH53_10400"
     CDS             complement(577..1572)
                     /gene="sbnA"
                     /locus_tag="VHH53_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HEX2290579.1"
                     /translation="MTVAGNAIEYLQDSVLACVGKTPLITLSRLFPEPDVEVLAKLEL
                     MNPGGSSKDRPARYIIEKGLADGSIPAGAHLVESSSGNFGIALAITARLHGLTLTCVV
                     DPKATSANIAILRQLGVDIDIVHEPDDAGGYLQTRVRRVQELLHEMPAAVWINQYANK
                     RNWQAHYHGTGAEMCEQLARPPDYLFAAVSTTGSILGCARRLRERFPDLRVIAVDAAG
                     SIIFGAPPGRREIPGIGASRVPELLAPEEIDEVVHVSDLESAQACRAVLETEGIFAGG
                     STGSVVAAISKTLPSLPRPCRIVTIFPDRGDRYLDLIYDDAWLAKLQHRDLGAAE"
BASE COUNT          277 a          551 c          541 g          271 t
ORIGIN      
        1 ccgcaacggc ccgtaccggg ccagctcagc cgacgtccag ccgaccgtgg cgtgggcggt
       61 cgggtgaaac ggccggttac gggtggcggc gagccgttcc gcagtgagca gctcaccggg
      121 gcgtggccga gcgtcggcca agcgggcccg cctggcgcgc accactgtcg cgtgctgcac
      181 cgccgtgcgc aggcccgcac cgccctgcct gacatgggac gagtccgagc agtcacctgc
      241 cagcagcgcc agcagttcat cgggccgcag ctgccgcggc ggcgatccgg acccgtcacc
      301 gtgccagacc ggcgggcggg agaaccggca cggctgcaag gcccccgcga tgcgggcccg
      361 gaaacacacc catccactgg ccacgtcgat gcggcaccac cactcgtcag cttccacggt
      421 ctcggtgccc agcgccgcgg gccggcatcg ctgtgcggcg tctgcgaacc cccagaggtt
      481 ctcctggatc agtgtgtcaa ccagatcccg catcacaagc tcggctggct ggtcgtttcg
      541 ggaggcacgg atcacggctg ctccgtcagt gcgacgtcac tcggccgcgc cgaggtcgcg
      601 gtgttgcagc ttggccagcc atgcgtcgtc gtagatgagg tcgagatagc gatccccgcg
      661 gtcggggaag atcgtgacga tgcggcaggg cctgggcagg ctgggcagcg tcttggagat
      721 cgcggccacg actgacccgg tggagccacc cgcgaagatg ccctcggtct ccaggacagc
      781 gcggcaggcc tgcgcggact cgaggtcact cacatggaca acctcgtcga tttcttccgg
      841 cgcgagcagc tccggcacgc ggctggcgcc gataccggga atctcgcggc gaccaggcgg
      901 cgctccgaag ataatggaac cagccgcgtc tactgcgatg acgcgcaaat cagggaagcg
      961 ctcccgcagc cgtcgggcac agccgagaat gctgccggtc gtactgaccg cagcgaacaa
     1021 gtaatccggt ggtcgcgcga gctgctcgca catctctgct ccggtgccgt ggtaatgggc
     1081 ctgccagttg cgcttgttgg cgtactggtt gatccatacc gcagcgggca tctcgtgcag
     1141 cagctcctgc acgcgacgga cccgggtctg caggtatcct cctgcgtcgt cgggctcatg
     1201 gacgatatcg atatccacac cgagctggcg caggatcgcg atgttcgcag acgtcgcctt
     1261 gggatcgacc acgcacgtca gtgtcagtcc gtgaagccgg gcagtgatcg ccaacgcgat
     1321 accgaaattc ccggagctgc tttccacgag atgcgctccc gcaggaatgg aaccgtccgc
     1381 gagacctttt tcgatgatgt atcgggcggg gcggtccttt gaactcccac cggggttcat
     1441 gagttccagt ttggcgagaa cctccacatc gggctctgga aacagccggc tcagcgttat
     1501 cagcggcgtc tttcctacac acgccagcac ggagtcctgg aggtattcga tggcgtttcc
     1561 ggcgacggtc atgcgactcc ctcgctgctg caagctgaag cttcttcacc acagagacga
     1621 ctgactagcc tcgcgacctg
//
LOCUS       DATADN010000800         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1292761, whole genome shotgun sequence.
ACCESSION   DATADN010000800 DATADN010000000
VERSION     DATADN010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292761"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHH53_10405"
     CDS             <1..232
                     /locus_tag="VHH53_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX2290580.1"
                     /translation="VRRWVKPEEFVEHARIGTLMGFAGVMAGPLVRSSYRAGRLYAQA
                     IAHRGDQIPEHLAHLATAGPAAQEASNLLARR"
     gene            304..1068
                     /locus_tag="VHH53_10410"
     CDS             304..1068
                     /locus_tag="VHH53_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HEX2290581.1"
                     /translation="MAAKLPSKPSSATSKTKDKGAAKAAKRERRAASKQRRAQIIQAF
                     KVQRREDKALLPLMVGAFLGITAVGVGLGFLLGQLWFSVPMSITIGILAAIMIFGRRV
                     QRNVFAKAEGQPGAAAWALDNLRGRWKVTPAVAGTGQLDAVHRVLGRPGVVLVGEGAA
                     HRLKPLLAQEKKRVSRLIGETPIYDVTVGNGEGQIPLAKLPRHLAKLPRNINAAQIDA
                     LEGKLAILTTRAAAMPKGPLPQGAKMRNVQRTVRRR"
     gene            complement(1163..1621)
                     /locus_tag="VHH53_10415"
     CDS             complement(1163..1621)
                     /locus_tag="VHH53_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290582.1"
                     /translation="MSRWTQTWLAGTRESADGSAAGTAGQDYPGQRLGLPAHGPGSAA
                     GFGPRILAIMIDWLPCAAAAQLLTDNPSFSALALFAAVTALSIGVAGRTVGHAVAGLR
                     VGLLDGRRAGFGAGVIRTVLLCLVIPPVVYDADGRGLHDRAAGTIVLRTR"
     gene            1786..>2819
                     /gene="glnA"
                     /locus_tag="VHH53_10420"
     CDS             1786..>2819
                     /gene="glnA"
                     /locus_tag="VHH53_10420"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX2290583.1"
                     /translation="MFSNSDEVLRFIADEDVEFIDVRFCDLPGVMQHFTVPAAFFDAD
                     IIEEGLAFDGSSVRGFQSIHESDMLLLPDPATARIDPFRVQKTLNINFFVHDPFTREP
                     YSRDPRNVARKAEEFLASTGIADTAYFGPEAEFYCFDSVRFDTTPNSAFHEIDSVAGW
                     WNTGAEENGRNQGYKVNYKSGYFPVPPTDHFADLRNAMVTNLIRCGFTVEKAHHEVGT
                     AGQAEINYRYNTLLHAADDLQLYKYIVKNTAWAAGRTVTFMPKPLFGDNGSGMHTHQS
                     LWTDGAPLFHDESGYAGLSDIGRHYIGGILHHAPSLLAFTNPTVNSYHRLVPGFEAPV
                     NLVYSQRNRSA"
BASE COUNT          522 a          956 c          898 g          443 t
ORIGIN      
        1 ggtgcggcgg tgggtcaagc ccgaagagtt cgtcgagcac gcccgcatcg gcaccctgat
       61 gggattcgcc ggggtgatgg ccgggccgtt ggtccgatcg tcctataggg caggccggct
      121 ctatgcccaa gcgatcgcgc accgcggcga tcagataccc gagcacctcg cgcacctggc
      181 cacggcaggc ccggcggcgc aggaggcgtc caacctgctt gcccgccgtt gacagcacta
      241 gtcagcactc gttggcgcta gtcggctggt cattcaccac gcgttgcgcc gtatccttgg
      301 cccatggccg ccaagcttcc ctcgaaaccc tcctcggcaa cctcgaagac caaggacaag
      361 ggagcggcga aggccgccaa gcgggagcgc cgcgccgcat cgaagcagcg ccgggctcag
      421 atcatccagg ccttcaaagt ccagcgccgc gaggacaagg cgctgttgcc gttgatggtc
      481 ggagcattcc tgggcatcac cgcggtcggg gttgggctgg gtttcctgct cggccagctg
      541 tggttctcgg taccgatgag catcaccatc ggcatactcg cggcaataat gatcttcggg
      601 cggcgggtgc agcgcaatgt gttcgccaag gccgagggcc agcccggtgc cgcggcctgg
      661 gcgctggaca acctgcgcgg ccggtggaag gtgacgccgg ccgtcgcggg caccggccaa
      721 ctcgacgccg tgcacagggt tctcgggcgg cccggtgtcg tgctggtcgg cgagggagcc
      781 gcacatcggc tcaaaccgct gctggcgcag gagaagaagc gggtgtcccg gctgatcggg
      841 gagactccga tctacgacgt cacggtgggc aacggcgaag gccagattcc actggccaaa
      901 ctgccacggc acctcgcgaa gttaccgcgc aacatcaatg cagcgcagat cgacgcgctg
      961 gaaggcaagc tggccatcct taccacccga gctgccgcga tgcccaaggg cccgttgccg
     1021 cagggcgcca agatgcgcaa cgtgcaacgc accgttcgcc gccgctgagc cctttacccg
     1081 gcatggagac ggatcggggt tcttgggagc cgatttacct cgacatgtct ccaggttggg
     1141 ggtagccgag ttgagaatgc gctcagcggg tccgcagcac gatggtcccg gcggcgcggt
     1201 cgtgcaggcc gcggccatcg gcgtcgtata ccaccggggg gatgactaga cacagcagca
     1261 cggtgcggat caccccggca ccaaagccgg cccgacgccc gtccagcagc cctacccgca
     1321 gtcccgccac agcatggccg accgtgcggc ctgcgacccc gatggacagt gcggtcaccg
     1381 ctgcgaacag cgccaacgcc gagaacgacg ggttgtcagt gagcagctgg gcggccgcgg
     1441 cgcaaggcag ccagtcgatc atgatggcca ggatccgggg accgaagccg gcggccgaac
     1501 cgggtccatg cgcaggcaag cccaacctct gaccgggata gtcctgccca gcagtgcctg
     1561 cggcggagcc atctgcggat tcccgagtgc cggccagcca ggtttgcgtc cacctgctca
     1621 cctgaccagg gtaggccgca cagggctagc atcggtcacc accaccctcg taaccttggc
     1681 gaaacaaccg ggtgatgggt gggtaacacc gccctcttag gttcgccgca gcgaactgac
     1741 cgattgcccc ggtaaacagc actgtgcacg aaggagcgaa cgacgttgtt ctccaactcc
     1801 gatgaggtcc tgcgcttcat cgccgacgag gacgtcgagt tcatcgacgt ccgcttctgc
     1861 gacctgccgg gcgtcatgca gcacttcaca gtcccggccg cgttcttcga cgcagacatc
     1921 atcgaggagg gcctggcctt cgacggctcg tcggtacgcg ggttccagtc gattcatgag
     1981 tcggacatgc tgctgctgcc cgaccctgcg accgcgcgca tcgatccgtt ccgggtgcag
     2041 aagacgctga acatcaactt cttcgtgcac gacccgttca ctcgcgagcc ctacagccgc
     2101 gacccgcgca acgtggcgcg caaggccgaa gagttcctgg cttcgaccgg catcgccgat
     2161 actgcgtact tcggtcccga ggcggagttc tactgctttg actcggtgcg cttcgacacc
     2221 acacccaaca gcgcgttcca tgagatcgac tcggtcgccg gttggtggaa caccggcgcc
     2281 gaggagaacg gccgcaacca aggatacaag gtcaactaca agagcggtta cttcccggtg
     2341 ccgccgaccg accacttcgc cgatctgcgc aatgccatgg tgacgaacct catcaggtgc
     2401 ggcttcaccg tggagaaagc gcaccatgag gtcggcaccg caggccaggc agagatcaac
     2461 tacaggtaca acaccctgct gcacgccgcc gatgacctgc agctctacaa gtacatcgtc
     2521 aagaacaccg catgggcggc cggacggacc gtgacattca tgcccaagcc gcttttcggg
     2581 gacaacggct ccggcatgca cacccaccag tcgctgtgga ccgacggtgc gcccctgttc
     2641 cacgacgagt ccggctacgc cggactgtcc gacatcgggc ggcactacat cggtggcatc
     2701 ctgcaccatg caccatcatt gctggcgttc accaacccga ccgtgaactc ctaccaccgc
     2761 ctggtgcctg ggttcgaagc gccggtgaac ctggtgtact cgcagcgcaa ccggtcggc
//
LOCUS       DATADN010000801         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_408951, whole genome shotgun sequence.
ACCESSION   DATADN010000801 DATADN010000000
VERSION     DATADN010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408951"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..95
                     /locus_tag="VHH53_10425"
                     /pseudo
     CDS             3..95
                     /locus_tag="VHH53_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(90..254)
                     /locus_tag="VHH53_10430"
                     /pseudo
     CDS             complement(90..254)
                     /locus_tag="VHH53_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            complement(251..>2503)
                     /locus_tag="VHH53_10435"
     CDS             complement(251..>2503)
                     /locus_tag="VHH53_10435"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX2290584.1"
                     /translation="NADMREYLQRLLSPRYRVHAVGDGLAALAAAQAQRPDLIVSDVM
                     MPTLDGLSLVTALRSDANTAQVPVLLLSARAGQEAAIEGLDVGADDYLVKPFTAQELL
                     ARVRSNLELARLRTQESTWRSTLISAMQNGMFVADPDGTIVELNDAFADILGYGADDL
                     PYVRPYPWWPDPVQEPEEFAQVHAASETAMRFGSDGRWVLPMRHGVDGRRVWLSIASG
                     VVTHPDGTIQAFVGTVRDVTAEHLTARRDAAVARLTGRLGEASDVQGVLTAGLADLAA
                     NWITRCALMFTWDSADKVSVIGTGTSWAALPPATRAAIIRTRTTGRVHWQQGDRSVEP
                     PAPTVAGVPVLWGTQVNVIWLEVDPGWPFGSEDAALLSDLAGHLGHALSRARLFDEQR
                     TASLTLQRAILGPTVLPTGFAVRYEPAISQLAVGGDWYDIVELGADRVGVAVGDCVGH
                     GLAAAAVMGQLRTACRTLLLQDHTAAQVLAAMDHVAVLIPDAVCTTLLCAIIDRTTGT
                     MIYSCAGHPPAIVARADGRTELLDDARSVPLATVDVARPEATAVLATGDTVLLYTDGL
                     VERRNIVLDTGISRARDVLAQTHHLAPAEIADCLAEQLLSDGHDDDVAYLLYQHPLGV
                     GRTVTATVPARPEQLAILRRTLRQWLSATAADQNTAHELLLAAGEAVTNAVEHAYLDS
                     EPGSVELTVSRYAEELHLEIRDHGRWRTIPAPGPRGRGLKMMKKLTDAVTVDIDDHGT
                     TVRLHKRLLL"
BASE COUNT          419 a          821 c          864 g          399 t
ORIGIN      
        1 agctgaagat tcacgagtac cgcgttgcga tgacgccggc cggcgtcatc gagctcaccc
       61 gtcgcgggca tgacgtcatc gtggaacgcg gcgcccgctg ccagataggt ctcgtcgggg
      121 aatgcgcagc cggcaccggc gccgcgttcc acgatgacgt catgcccgcg acgggtgagc
      181 tcgaggacgc cggccggcgt catcgcaacg cggtactcgt gaatcttcag ctcccgcggg
      241 ataccgatct tcacagcagc agcctcttgt ggagccggac ggtggtgccg tggtcgtcga
      301 tgtccacagt gacggcatcg gtgagttttt tcatcatctt gaggccgcgc ccgcgtgggc
      361 cgggggccgg gatggtgcgc cagcggccgt ggtcgcggat ctcaaggtgg agctcctcgg
      421 cgtagcggct gacggtgagc tcaacgctgc cgggctcgct gtcaaggtaa gcgtgttcaa
      481 cggcgttggt gacagcttca ccggcggcga gcaacagctc gtgggcggtg ttctggtcgg
      541 cggcggtcgc gctcagccat tggcgcagcg tgcggcgcag gatagcaagc tgctcagggc
      601 gagccggcac cgtggcggtg actgtgcggc ccactccgag agggtgctgg tagagcaggt
      661 aggcgacgtc gtcgtcgtga ccatcgctga ggagttgttc ggcgagacag tcggcgatct
      721 cagcaggcgc caggtgatgg gtctgggcca gcacgtctcg agcacggctg atgccggtat
      781 cgaggacgat gttgcgccgt tcgaccaacc catcggtgta gaggagcacg gtgtcacccg
      841 tagccaggac ggcggtggcc tccggtcgcg caacgtcgac ggtggccaag ggaacgctgc
      901 gggcgtcatc aaggagctcg gtgcggccat cggctctggc cacgatggcc ggcgggtgac
      961 cggcgcagct gtagatcata gtgccggtgg tgcggtcgat gatggcgcac aacagggtgg
     1021 tgcagaccgc gtcggggatc aacacagcaa catgatccat cgccgccagt acctgtgcgg
     1081 cggtgtgatc ttgcagcagc agggtgcggc aggccgtgcg aagctgcccc atcacggcgg
     1141 cggcggccaa cccatggcca acgcagtcgc cgacggccac acccacccga tccgcaccca
     1201 attcgacaat gtcgtaccag tcacccccga ccgccagctg gctgatcgca ggctcataac
     1261 gcaccgcgaa accggtcggc agcacggtgg gtccgagaat ggcgcgttgc aaggtgagtg
     1321 acgcagtgcg ttgttcgtcg aacaaccgtg ccctgctcag cgcgtgaccg aggtgaccag
     1381 cgaggtccga cagcagcgcg gcatcctcgc tgccgaaggg ccagcctgga tcaacctcaa
     1441 gccagatcac gttgacctgg gtgccccaga gaacggggac acccgccacg gtgggcgcag
     1501 gtggttccac cgaccggtcg ccctgctgcc agtgcactcg cccggtggtg cgggttcgaa
     1561 tgatcgcggc cctggtcgcc ggcggcagcg cggcccacga cgtgccggta ccgatcaccg
     1621 ataccttgtc ggcgctgtcc caggtgaaca tcagggcgca gcgggtgatc cagtttgcag
     1681 ccagatcggc caacccggcg gtcaacacgc cctgcacgtc gctggcttcg cccagccgcc
     1741 cggtgagccg cgccacggcc gcgtcgcggc gcgcagtgag gtgttcggcg gtgacgtcgc
     1801 gcaccgtgcc cacaaacgcc tggatggtgc cgtcaggatg ggtgaccacg ccgctggcga
     1861 tcgagagcca gacccgccga ccgtccacac catgccgcat cggcaacacc caccggccat
     1921 cgctgccaaa ccgcatcgcg gtctcggagg cggcgtgcac ctgcgcgaat tcctcgggct
     1981 cctgaaccgg atcgggccac cacgggtacg gccgaacata gggtaggtcg tcagcgccgt
     2041 agccgaggat atcggcgaac gcgtcgttga gctcgacgat ggtgccgtct ggatctgcga
     2101 cgaacatgcc gttctgcatc gcgctgatca gggtggaccg ccacgtcgat tcctgggtgc
     2161 gcagcctggc cagttccagg ttcgaccgca ctcgagccag cagctcctgc gcagtgaagg
     2221 gcttgactag gtaatcgtcc gcgccgacgt ccagtccctc gatcgcggcc tcctgcccgg
     2281 cccgcgccga gagcagcagc accggcactt gtgcggtgtt cgcatccgac cgcaacgccg
     2341 tgaccaagct caagccgtcc agagtcggca tcatgacgtc actgacgatc aagtcgggtc
     2401 gttgtgcttg cgccgccgcc aatgcggcga gcccatcgcc gaccgcgtgc acgcgatagc
     2461 gcggcgacag caaccgctgt aggtactccc gcatgtcggc gtt
//
LOCUS       DATADN010000802         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_409041, whole genome shotgun sequence.
ACCESSION   DATADN010000802 DATADN010000000
VERSION     DATADN010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409041"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VHH53_10440"
     CDS             complement(<1..724)
                     /locus_tag="VHH53_10440"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX2290585.1"
                     /translation="MRDLVEIGMGRTARRAYTFDDVEIVPSRRTRSSRDVSTTWQIDA
                     YRFDIPLITHPTDAVVSPAMAIQIGELGGLGVLNAEGLWARHPNVDKLLYALTSAMDE
                     ADETSAVRLLQQWHSATIRVELLTEAIGQVAKSGVTVAARVSPQHARDLAPDLLAAGV
                     EILIVQGTIVSAEHVSSAAEPLNLVEFIASMDVPVIVGGVGDYRTAMHLMRTGAAGVI
                     VGYGQSQASTTNRVLGIGVPMAT"
     gene            complement(726..>1627)
                     /locus_tag="VHH53_10445"
     CDS             complement(726..>1627)
                     /locus_tag="VHH53_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX2290586.1"
                     /translation="ITVKDFVKTEKYPLATKDPDGRLLVGAAVGVGEGGKQRAALLVE
                     AGVDVLVVDTAHGHSRGVLQMISTLKAELGSTVDIIGGNVATRAGALALVEAGADAVK
                     VGVGPGSICTTRVVAGVGVPQISAIFEADRACRPAGVPVIGDGGIQYSGDIAKAIVAG
                     ASTVMLGSLLGGTAEAPGDLILVDGKQYKTYRGMGSVGAMALRGAGRSYSRDRYFADD
                     VLSDDKLVPEGIEGRVPFRGPLSLVAHQLVGGLRAAMGYAGAATIPALQSAQLVQITA
                     AGIKESHPHDITMTVEAPNYAVR"
BASE COUNT          269 a          583 c          520 g          255 t
ORIGIN      
        1 cggtggccat cggcactccg atgcccagca cccggttggt ggtgctggcc tgggactggc
       61 cgtagccgac gatgaccccg gccgcgccgg tgcgcatcag gtgcatcgcg gtgcggtagt
      121 cgcccactcc cccgacgatc accgggacgt ccatcgacgc gatgaattct acgaggttga
      181 gcggctcggc agcgctggag acatgctcgg ccgagacgat ggtgccttgc acgataagga
      241 tctcgacacc agcggcgagc aggtcaggcg cgagatcgcg ggcatgctgc gggctgaccc
      301 gggccgccac tgtcacgccg gacttggcaa cctgaccgat cgcctcggtg agcagctcaa
      361 cccggatcgt cgccgagtgc cactgttgca gcagccgcac cgccgacgtc tcgtcggcct
      421 cgtccatcgc ggacgtgagc gcgtacagca acttgtcgac gttcggatgg cgcgcccaca
      481 gtccttcggc attgagtacg cccaacccac cgagctcacc gatctggatc gccatcgccg
      541 gcgacacgac cgcatcggtg ggatgggtaa tcagcgggat gtcgaagcgg taggcgtcga
      601 tctgccaggt ggtggaaacg tcccgggagg agcgagtacg ccgcgagggc acgatctcca
      661 catcatcgaa ggtgtaggcc cgccgcgcgg tgcgacccat gccgatctcc accaggtccc
      721 gcatctcagc gcaccgcgta gtttggggcc tccaccgtca tggtgatgtc gtgtgggtgg
      781 ctctccttga tcccggccgc cgtgatctgc accagctgcg cgctttgcaa cgccgggatc
      841 gtcgcagcac cggcgtaacc catcgctgcc cgcaggccac cgaccagctg atgggccacc
      901 agcgacaacg gcccgcggaa ggggacccgc ccctcgatgc cctcgggcac cagcttgtcg
      961 tcggacagca cgtcgtcggc gaagtagcga tccctggagt acgaccgtcc ggcaccgcgc
     1021 aaggccatcg caccgaccga acccatcccg cgataggtct tgtactgctt gccgtcgacc
     1081 aggatcagat cgccgggcgc ctcggcggtg cccccgagca ggctgcccag catcacggtg
     1141 ctggcgcccg cgacgatggc cttggcgata tcgccggagt actggatgcc gccgtcaccg
     1201 atcaccggaa cacctgcggg tcggcacgcc cggtcagcct cgaagatggc gctgatctgg
     1261 ggtacgccca ccccagccac aacccgggtg gtgcagatgg agccaggacc caccccgacc
     1321 ttgaccgcat cggcgccggc ctcgaccagc gccaatgccc cggcccgggt ggcgacgttg
     1381 ccgccgatga tgtcgaccgt gctgcccagc tcggccttga gcgtgctgat catctgcagt
     1441 accccgcggg agtgcccgtg cgcggtgtcc acgacgagca cgtcaacgcc ggcctcgacg
     1501 agcaacgccg ctcgctgctt gccaccctcg ccaaccccga cagcagctcc caccagcagt
     1561 cgcccgtctg gatccttggt cgccagcgga tacttctcgg ttttcacgaa gtccttgacg
     1621 gtgatca
//
LOCUS       DATADN010000803         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_842033, whole genome shotgun sequence.
ACCESSION   DATADN010000803 DATADN010000000
VERSION     DATADN010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842033"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VHH53_10450"
     CDS             complement(<1..390)
                     /locus_tag="VHH53_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290587.1"
                     /translation="MLDYEIFELGDVRLQMGETLRDARLAYRTYGSLNEAKSNAIVYP
                     TWYSGQHYDNEWLIGEGMALDPTKYFIIIPNMLGNGLSSSPSNTPPPYDRARFPHVTV
                     YDNVSVQHRLLTEHFGIERLELVTGWSM"
     gene            complement(508..1626)
                     /gene="yut"
                     /locus_tag="VHH53_10455"
     CDS             complement(508..1626)
                     /gene="yut"
                     /locus_tag="VHH53_10455"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HEX2290588.1"
                     /translation="MAAASAPRRAADTWARLAQTNPALASIDALLRGTGQVMFQNNPL
                     TGLLFLVGIFVNSAKLGGAGLLGLAVSTLTAYLLGADRALIRNGLFGFNGILVGIGLA
                     FFLEFEPLLAVYIVLGGAVSTILMMALANFLGPWDMPALTAPFVLTAWLLLFAVYQFA
                     NIRPSPLIAPMPLDPQATIQTELRELPTGPGNAGLTAANLAHGFFRGVGEVMFQDNLL
                     TGVIFLLAILVNSRISAGFAALGSLLGLLTALALGGNGVLIYHGLYGFNPVLCAIALG
                     GLFFVLTWRSAAYALVAAVFSAIVFAAFVVLLSPIGMPALTAPFVLVTWLFLLPKGAF
                     NTLQPVALAEVSTAENTRASYRVREAGPSATPEPGEAT"
     gene            complement(1813..3060)
                     /gene="fmdA"
                     /locus_tag="VHH53_10460"
     CDS             complement(1813..3060)
                     /gene="fmdA"
                     /locus_tag="VHH53_10460"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HEX2290589.1"
                     /translation="MPEVVFRVDQSKSMRDQDSPGHNRWHPDIPSAAFVQPGNEFRVE
                     CREWTDAQLGNNDSANDVRDVDLSICHMLSGPIEVQGAEPGDLLVVDILDLGPVPQET
                     GPAPGQGWGYTGVFAKANGGGLLTDWLPDSYKANWDFHGQDATSRHLPGIRYTGITHP
                     GLFGTAPSAELLAKWNDRERALVATDPDRVPPLALVPLEQGTLAGRLTGSELDKVARE
                     GARTVPARENGGNHDIKNFTRGSRVFYPVHVPGAKFSGGDLHFSQGDGEITFCGAIEM
                     GGFIDFGVDLIKGGMEKYGISTNPVFMPGRVEPRYTEFLSFIGISVDHDSNTNYYMNA
                     TLAYKRACLNAIEYLKTFGYSGEQAYLLLGSAPIEGRISGIVDIPNACCSLYLPTAIF
                     DFDIRPTAEGPRRADRGQCAVTS"
     gene            complement(3359..3643)
                     /locus_tag="VHH53_10465"
     CDS             complement(3359..3643)
                     /locus_tag="VHH53_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2290590.1"
                     /translation="MATYEYRCEDCGGFEQRMAMGTATASAACPTCGRDAQRVFSVPM
                     TSRTPRPLATMLAREEASRDYPEVVDRLPPRRRPRRSAPPNPALARLPKP"
BASE COUNT          625 a         1163 c         1325 g          597 t
ORIGIN      
        1 catcgaccag ccggttacca gctccaacct ctcgataccg aagtgctccg tgagaagcct
       61 gtgctgcacc gacacgttgt cgtagaccgt cacgtggggg aagcgggccc tgtcgtaggg
      121 cggcggggta ttgcttggcg aggacgatag cccgttgccc agcatattcg ggatgatgat
      181 gaagtatttc gtcgggtcga gcgccatccc ctccccgatc agccactcgt tgtcgtagtg
      241 ctgtccggag taccaggtgg gatagacgat ggcgttgctc ttggcctcgt tgagcgaccc
      301 gtaggtccgg taggccagcc tcgcgtcgcg gagggtctcg cccatctgca gccgaacatc
      361 accgagttcg aagatctcgt agtcgagcat ggcctctcct tccctggtgc cagccgacag
      421 gatggtcctg agcgccagcc gccagaacca ggcgccgtac tgttggacct gggtcttggc
      481 aggccgcgac ctgccaggtt ggcgtgctca tgtcgcctcg ccgggctcag gggtggcgct
      541 cgggcctgct tcacggaccc ggtagctggc acgggtgttc tcagcggtgc tgacctcggc
      601 gagcgcgacc ggctggaggg tgttgaatgc gcccttgggc aacaagaaca gccaggtcac
      661 cagcacgaag ggggcggtca gggccggcat gccgatcggg gacagcagca cgacaaaggc
      721 agcgaacaca atggcgctga acacggcagc aaccagcgcg taagcagccg acctccaggt
      781 cagcacgaag aacaagccac cgagggcgat ggcgcacagt accgggttga agccgtacag
      841 cccgtggtag atcaggacgc cgttgccgcc caatgccagc gccgtgagca gtcccagcag
      901 cgaacccagc gcggcgaacc ccgccgagat gcgggaattg accaggatag ccagcaggaa
      961 gatcaccccg gtcagcaggt tgtcctgaaa catcacttcc ccgacgccgc ggaagaagcc
     1021 atgggccagg ttggcggcgg tcaggcctgc attccctgga ccggtcggga gctcccgaag
     1081 ctcggtctgg atggtcgcct gcgggtccag cggcatcggg gcgatcagtg ggctgggccg
     1141 gatgttggcg aactggtaga cggcaaacag cagcagccaa gctgtgagca caaagggcgc
     1201 cgtcagcgcc ggcatgtccc agggacccag gaagttggcc agcgccatca tgaggatggt
     1261 ggagacggcc ccgccaagca cgatgtagac ggccagcagc ggctcgaact cgaggaagaa
     1321 ggccaggccg atcccgacca ggatgccgtt gaagccgaac agcccgttgc ggatcagggc
     1381 acggtcggcg ccgagcaggt aggccgtcag ggtgctgacc gccaggccaa gcagtcccgc
     1441 gccaccgagc ttggccgagt tgacgaagat accgaccagg aagagcagcc cggtcagggg
     1501 attgttctgg aacatcacct gcccagtgcc gcgaaggagg gcgtcgatgc ttgcgagcgc
     1561 tgggttggtc tgggccagcc tggcccaggt gtctgcggcg cgtcgtggcg cgcttgctgc
     1621 tgccatggct ttggctccat cgatggctcg gccggatgcg gccgagcggc aggtcggcgc
     1681 ggcccggcgg tcagacccgg caccgatgcg ccgccggcta gccgtcgggt gcgcgtgccg
     1741 acctgttagc cgttgctttg gccgggcgct cgcagtcgtg gctcccgctc cgggacgccc
     1801 ggccggctgt ggttacgacg tcacggcgca ctgtcctcgg tcggccctcc tggggccctc
     1861 ggcagtgggt cggatgtcga agtcgaagat cgccgtcggg aggtagagcg agcagcaggc
     1921 gttagggatg tcaacgatgc cgctgatgcg gccctcgatc ggggccgagc cgagcagcag
     1981 gtaggcctgc tcgccggagt agccgaaggt cttgaggtac tcgatggcgt tcaggcaggc
     2041 ccgcttgtag gccagcgtcg cgttcatgta gtagttcgtg ttggagtcat ggtccaccga
     2101 gatcccgatg aacgacagga attcggtgta cctgggctcg acgcggccgg gcatgaacac
     2161 cgggttggtg gagatcccat atttctccat cccacccttg atcaggtcca cgccgaagtc
     2221 gatgaagccg cccatctcga tcgcgccgca gaaggtgatc tccccgtccc cctggctgaa
     2281 gtgcaggtcg ccaccggaga acttggcccc ggggacatgc accggataga agacgcggct
     2341 gccgcgggtg aagttcttga tgtcgtggtt gccgccgttc tcgcgggcgg gcacggtgcg
     2401 ggcgccctcc cgagcgacct tgtccaattc tgaacccgtc agtctgccag caagcgtgcc
     2461 ctgctccagc gggacaagcg ccagcggcgg gacgcggtcg gggtcggtgg cgaccagcgc
     2521 ccgctcccgg tcgttccatt tcgccagcag ctccgccgag ggcgcggtgc cgaacagtcc
     2581 tgggtgggtg atgccggtgt agcggatgcc cggcaggtgc cgggaggtgg cgtcctggcc
     2641 gtggaagtcc cagttcgcct tgtaggagtc cggcaaccaa tcggtgagca acccgccacc
     2701 attggccttg gcaaacacgc cggtgtagcc ccagccctgg ccgggggccg gtccggtctc
     2761 ctgggggacc gggccgaggt cgaggatgtc gaccaccagc aggtcgcccg gttcagcgcc
     2821 ctggacctcg atcgggccgc tgagcatgtg acagatcgac aggtcgacgt cacgcacgtc
     2881 attggcggag tcgttgttgc cgagctgggc gtcggtccac tcccggcact cgacccggaa
     2941 ctcattgccg ggctggacga aggcggcact tgggatgtcg ggatgccagc ggttgtggcc
     3001 gggggaatcc tggtcgcgca tggacttgga ctggtcgacg cggaagacga cctctggcat
     3061 aagcggcact tccttgctgg tgcgacgtgg atgcgctggc gtgcctggtg tattcaccct
     3121 ctcactgcag ccgcgggccg ggggggagcg ggcacgagcg ccacgccggc gcacagtcac
     3181 accggccgct cgggcgcggt aggtgagggg ctcggtcgcc gaaaagaccg gcatgcgctc
     3241 tggatctcct ccggtccggt ccgagcaggt ggtcagggac ggtgctggcg agactgggcg
     3301 ctcaccggga agcaatccga gccacgcgca gcagctcggc gctcgacgcg acggctgcct
     3361 agggtttggg taggcgggcg agggcaggat tcggtggggc ggaacgtcgt ggtcgtcggc
     3421 gcgggggcag ccggtcgacc acctccgggt agtcgcggct tgcctcctca cgggccagca
     3481 tcgtggcaag aggcctaggt gtccgagacg tcattggcac agagaagacc cgctgggcgt
     3541 cccgaccgca cgtcgggcag gccgcagacg ccgtggctgt gcccatagcc atccgctgct
     3601 caaaaccgcc gcagtcctcg cagcgatact cgtaggtggc cacgcaaccc tccagggaag
     3661 caacggtcgg aggcgtcggg accggaagct agttctatgc ccacgcatcc
//
LOCUS       DATADN010000804         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_240539, whole genome shotgun sequence.
ACCESSION   DATADN010000804 DATADN010000000
VERSION     DATADN010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240539"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..169
                     /locus_tag="VHH53_10470"
     CDS             17..169
                     /locus_tag="VHH53_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290591.1"
                     /translation="MAGSGIGLAGARRIVELHGGAISVRSREGEGSTFTVRLPISAYD
                     QERSNR"
     gene            complement(148..1449)
                     /locus_tag="VHH53_10475"
     CDS             complement(148..1449)
                     /locus_tag="VHH53_10475"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2290592.1"
                     /translation="MVLGAGKGFMGNQVLNRISVRRRLPPKVAPSPDTGRVSRSLRVY
                     PAFRMLLLGTLATNSAFWMYQVCVGWLALQLLDSPLFVGLTGFVGGIPLLVVSLPAGV
                     MIDRYDRRTVLLVAQIAVMLLAAVFSLLVGTGAIAPWSTLALVAAYGTAMSFIFPTRT
                     TIVTMLVNRGDLANAVALNAAVQNATRVTGPALAGVLIAFIGLAGTFAVAALLQILAL
                     VTTLKLPASTRIGTARGGSAWASLTLGLRIVAQDAFLSSLILLAITTNVLVMPYITMM
                     PVFARDVLGIGSTGLGLLLASTGLGTVAGALAVAASRRLGSWSRVQVVTAATFTALVL
                     GFALTSNLPLAVLLLFAAGGMSAAFMALAQTALQLRVDDDVRGRVLSIYLATWGMLPF
                     GQLAVGVIAERTGAPLATAGACVVALVGIGLITRHYRLLRS"
BASE COUNT          335 a          512 c          505 g          197 t
ORIGIN      
        1 cgggaacgtc gagagcattg cgggaagcgg cattggtctg gcgggcgcca ggcggatcgt
       61 cgagctccat ggcggtgcca tctcggtccg gagcagagag ggagagggaa gcacttttac
      121 ggttcgcctc ccgatttccg cctacgatca ggagcgcagc aaccggtagt gacgggtgat
      181 gagcccgatg ccgaccagcg ccacgacgca ggcgccggct gtcgccagcg gcgcgccggt
      241 ccgctcggcg attacgccga cggccagttg accgaagggc agcatgcccc aggtcgccag
      301 gtagatcgaa agcacccgac cgcgcacgtc atcgtcgacc cgcaactgca gcgccgtctg
      361 ggccagggcc atgaaggcgg cgctcatccc cccggccgca aagagcagca atacggccag
      421 aggaagattc gaggtcagtg caaaacccag caccagcgcg gtaaacgtgg ccgcagtcac
      481 cacttggaca cgagaccacg atccgagacg acgcgaagcc gcgacggcga gcgctccggc
      541 gaccgttccc agcccggtgc tggccagcag taaccccaac ccggtcgagc caatacccag
      601 cacatcgcgg gcgaagaccg gcatcatcgt gatgtacggc atcaccagga cattcgtcgt
      661 gatcgccagc aggatcaggc tcgagaggaa cgcatcctga gccacgattc gcaacccgag
      721 cgtcaaactg gcccaggccg atccgccgcg agcggttccg atgcgtgtgc tggccggcaa
      781 cttgagggtc gtgacgagag ccagaatctg cagcagggca gccaccgcga acgtacccgc
      841 gaggccgatg aacgcgatca acacccctgc cagggctggt ccggtgacac gggtcgcatt
      901 ctggacagcc gcattgagcg ccaccgcgtt ggcgaggtcg ccgcggttga cgagcatcgt
      961 cacgatcgtg gtacgggtcg ggaagatgaa cgacatggca gtgccgtacg cggccaccag
     1021 cgccagggtc gaccagggcg cgatcgcccc ggtgccgacc agaagcgaaa acaccgcggc
     1081 gagcagcatc accgcgatct gggccactag cagcactgtg cgccgatcat agcgatcgat
     1141 catcacgccg gccggcaagg agaccacgag cagggggatg cccccgacaa agcccgtcaa
     1201 gccgacgaac agcggggagt ccagcagctg cagagcgagc caaccgacac agacctgata
     1261 catccagaag gcggagttcg ttgccagcgt tcccagcagc agcatccgga acgcggggta
     1321 gacccgcaat gagcgcgaca cgcggcctgt gtccggggaa ggcgcgacct tcggcggcag
     1381 gcgacgccgc acggagattc gatttagcac ctgattcccc atgaacccct tacccgctcc
     1441 caggaccacc tgtcactcgt atcgcgaagc tcggatcaat tcatggctca caccgggcgg
     1501 ctgtcccagc ggaagaagcg caccgcgatg gccatggcaa tgatgaagg
//
LOCUS       DATADN010000805         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_842122, whole genome shotgun sequence.
ACCESSION   DATADN010000805 DATADN010000000
VERSION     DATADN010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842122"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="VHH53_10480"
     CDS             complement(<1..1794)
                     /locus_tag="VHH53_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX2290593.1"
                     /translation="MTADAPVVPPRHDLRLVPAALAGWAVVLTGLHLGSLAAAASGAG
                     ALLAAGVAGLRGRSAAMLAVGGVTASLALVVGVQSWQVERHPVRAGAEGGLAATVSVQ
                     LSDDPQAVNSSGYGGRRPQPQLVVIRAELEAAELAGRQWRTGGRVVLLAPAQGWTGLL
                     PGQRVRATGLLAVPNRSDLTVAVLRVRGQPEVLAAPTAVQRVAERLRSGLRVAAGVLD
                     PELAGLLPALVVGDTSTMVPTVEDEFRAAGLAHLTAVSGANVAILCGAVLGLARLARA
                     GPRTAAILAGLVLVGFVVLCRPSPSVLRAAAMGAVTLMALMLGRRRSAVPALCAAVLV
                     LLLVDPALGGDPGFALSVLATGALVLLAPGWVAGLRRHGVPPGIAEALAVPAAAHVVT
                     APVVAGLSGQVSLVAVLANLLDAPAVAPATVFGVLAAVLGPLHPGAAAVVVHVAGPAV
                     GWLVGVGHHGAAVPDGVMQWPEGASGALLLALLVGAALFAMRAHRLRVLVAAGLVGAL
                     LVLVPTRYALPGWPATGWVMAACDVGQGDAVVLATAEPDRAVLVDAGSDPGPVSACLD
                     RLGIRRLALVVISHLHADHVGGLIGALRGRTA"
     gene            complement(1829..2668)
                     /locus_tag="VHH53_10485"
     CDS             complement(1829..2668)
                     /locus_tag="VHH53_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEX2290594.1"
                     /translation="MASLPSFPSTNTGDPQDARTRLMALRVPAEADDKEPPETVAADG
                     PPQTAPGDRIGSAATSQGGRLVRRWVPDSLRDARWEPGRPGALALSLVAALAAVIAAV
                     GVWRERPVPEPTLALPLVTADTTPGASPAPPPDAPEVVISVAGRVVRPGLVRLPDGTR
                     VADALAAAGGPLPGTDLMGLNIARRLSDGEQLLIGVAPPPGQSADGAAPSTPSGAPRK
                     VDLNTATLEQLDVLPGVGPVTAQRILDWRAAHGRFTSVNQLREVSGIGQARLARLKDL
                     VRV"
     gene            complement(2756..3625)
                     /locus_tag="VHH53_10490"
     CDS             complement(2756..3625)
                     /locus_tag="VHH53_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX2290595.1"
                     /translation="MPVAVVTDSTADLPDGLAQRHGIRQVPLHVMVNGRHRLDGLQLG
                     PADLVEALTRRATVRTSRPTPGEFEAVYTEVIRAGADTVVSVHLSRALSGTWESARLA
                     AEQIGAKRVRVVDSRSAGMGLGFAALAAATVAATDADQSTVYDTAERISARTRTFFSV
                     DTLEYLRRGGRISQAAALLGSALAVKPLLHVDSGRVVPLEKVRTATRARARLVELAVQ
                     AAGSEPLDLAVHHLGAADRATELVEQLHARLPALARCLISEVGAAIGAHAGPGMLGVI
                     VLPGGLTAQLPGG"
     gene            complement(3692..>4037)
                     /locus_tag="VHH53_10495"
     CDS             complement(3692..>4037)
                     /locus_tag="VHH53_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2290596.1"
                     /translation="GTWQGLTHAEVEVRCPGGIQTWRGNPEWAPPGGETRVEVAGRAA
                     RVVSELDAHGHSSAVLCTHGGLIAGLTPLLLGLPVSGWSVFGGISNCHWTVLLRHGLG
                     WRLFSYNTAAPV"
BASE COUNT          685 a         1542 c         1347 g          463 t
ORIGIN      
        1 ggcggtccgt ccccgcagcg cgccgatcag cccgcccacg tgatcggcgt gcaggtggct
       61 gatcacgacc agggcgagcc gccggatccc taggcggtca aggcaagccg acaccggacc
      121 cgggtcggat ccggcgtcga ccagcacagc gcgatccggc tccgcggtgg ccaggaccac
      181 cgcgtcgccc tggccgacgt cgcatgccgc catcacccag ccggtcgccg gccagcccgg
      241 caacgcatac cgcgtcggca ccagcacaag cagggcgccg accaacccgg ccgccacgag
      301 cacccgcagc cgatgtgccc gcatcgcgaa caacgccgcg ccaacgagca acgccaacag
      361 cagtgcaccg gaggcccctt ctggccactg catcaccccg tcgggaacag cggccccatg
      421 gtgacccaca cccaccaacc agcccactgc cggtccagcc acatgcacga cgacggcggc
      481 ggcaccgggg tgcaacggtc cgagcaccgc ggcgagtacg ccgaacacgg tcgccggtgc
      541 gaccgccggg gcgtccagca ggttggccag cactgctacc aggctcacct gcccggacag
      601 acctgccacc accggtgcgg tgaccacgtg ggcggcggcg ggcaccgcga gtgcctcggc
      661 aatcccgggc ggcaccccgt gacggcgcag tccggccacc caaccaggcg cgagcagcac
      721 cagcgcgccg gtggccagaa cggacagcgc gaaccccgga tcacccccta atgccggatc
      781 gaccaggagc aagaccagta ccgcagcgca caacgcggga actgccgagc gacggcggcc
      841 gagcatcagc gccatcaagg tgacggcgcc catcgcggcg gcccgcagca cactcggcga
      901 gggccggcac agcaccacga aaccgaccag gaccagccca gcgaggatgg ccgcggtccg
      961 cggtccagcc cgagccaacc gggccaaccc cagaacggcc ccacacaaga tcgccacgtt
     1021 ggcaccggac acggcggtga gatgcgccag accggcggcg cggaattcgt cttcgactgt
     1081 gggcaccatc gttgacgtgt cccccaccac caacgcaggc agcaacccgg ccagctccgg
     1141 atcgaggact ccggccgcca cccgcagccc ggaccgcaac cgctcggcaa cccgttggac
     1201 tgcggtgggc gccgccagca cctccggctg acctcgcacc cgtagcacgg caaccgtcag
     1261 gtcagaccgg ttgggtacgg cgagcagccc ggtggcccgc acccgctggc cgggcagcag
     1321 gccagtccag ccctgcgccg gagccagcag cacaacccga ccgccagtcc gccattgccg
     1381 cccggcgagc tccgcggcct caagctccgc gcgtatcacc accagctgcg gctgcggtcg
     1441 ccgaccgccg taacccgacg agttgacggc ctgcgggtcg tcgctcaact gcaccgatac
     1501 cgtcgccgcc aagccgcctt ccgctccggc tcgcaccgga tggcgctcaa cttgccacga
     1561 ctgcacaccc accacgagtg ccaatgacgc ggtcaccccg cccactgcca gcatcgctgc
     1621 cgaccggccg cgcagcccgg caacaccggc agccagcagg gcccccgcac ccgatgcggc
     1681 cgcggccagc gaccccaggt gcaacccggt aagcaccacc gcccagccgg ccagcgcggc
     1741 gggcacgaga cgcaggtcat gccgcggtgg gacaacgggt gcgtcggcgg tcattacgcc
     1801 gcctccgcgg gaaaaagcac gtcggtggtc acacccgcac cagatccttg agccgggcca
     1861 accgcgcctg accgatcccg ctcacctcac ggagctgatt caccgaagtg aagcggccgt
     1921 gcgcagcgcg ccagtcgaga atgcgctgcg cggtgaccgg gccgaccccg ggcagcacgt
     1981 cgagctgctc cagggtagcc gtgttgaggt cgaccttccg gggggcaccg gacggggtgc
     2041 tcggcgcggc gccgtccgcg gactgcccag gtggcggcgc cacaccgatc aggagctgct
     2101 cgccgtcaga cagccggcga gcgatgttca agcccatcag atcggtgccc ggcagtggcc
     2161 cgcccgccgc ggccagtgcg tcagctaccc gggtgccgtc gggtagccgg accagcccgg
     2221 gccgcaccac acgacccgcc acactgatca ccacctcagg ggcatccggc ggtggtgcag
     2281 gtgatgcgcc cggcgtcgtg tcagcggtga ccagcggcag ggctagggtc ggttcgggca
     2341 ccggacgttc ccgccacacc ccgaccgcgg cgatcactgc agccaatgcg gcgaccagcg
     2401 acagggcgag cgcgcctggg cgccctggtt cccaccgggc gtcccgcagc gaatccggca
     2461 cccagcgccg gaccagccgg cctccttgcg atgtggcggc tgagccgatc cggtcgcccg
     2521 gggcggtctg gggcggcccg tcggcagcaa ccgtctcggg cggttccttg tcgtcggcct
     2581 cggcgggcac tcgcagcgcc atgagccgcg ttcgggcgtc ctgcgggtcg ccggtgttgg
     2641 tggacgggaa gctgggaagc gaagccacgg gccgacgtta ggacggtccc gctattcgct
     2701 tcaccagctg cggccgtgag ctgtggacaa caggtcggct ggggacaagt cggtgtcagc
     2761 cgccggggag ctgcgccgtc aaaccaccgg gcaggacgat cacgccgagc attcccggtc
     2821 cagcatgcgc gccgatcgct gccccgacct cggagatgag gcatcgcgcc agtgccggca
     2881 gccgcgcatg caattgctcg accagttccg tcgcgcggtc ggcggcaccg aggtgatgca
     2941 ccgcgaggtc caacggctcc gaaccggctg cctgcaccgc cagttcgacc agccgagccc
     3001 gcgcgcgggt ggcggtgcga accttctcca gcggcacaac ccgtcccgag tcgacgtgca
     3061 gcagcggctt gaccgccaac gcgctgccca acagcgcggc cgcctggctg atcctgcctc
     3121 cacggcgcag gtactccagg gtgtccaccg agaagaaggt ccgggtgcga gcgctgattc
     3181 gctcagcagt gtcgtacacg gtgctctgat cggcgtcggt cgcggcgact gtggccgcag
     3241 cgagggcggc gaagcccaag cccatgccag cggagcgcga gtcgaccacc cgaacgcgct
     3301 tcgcgccgat ctgctcggcg gcaagtcgtg ccgactccca ggtgccggac aatgcgcggg
     3361 acagatgcac tgacacgacg gtgtcggcac cggccctgat gacctccgtg tacaccgcct
     3421 cgaactcccc cggtgtcggg cgagaggtac gcaccgtggc ccgtctcgtc agagcttcca
     3481 ccaagtcggc cggcccgagc tgcaggccgt ccaaccggtg gcggccgttc accatgacgt
     3541 gcagtggaac ctggcggata ccatggcgtt gcgcgagccc gtccgggaga tcagcggttg
     3601 agtcggtgac gacggcaacg ggcacgatca tcagcgtagg ggccgtcagc ggcccagcag
     3661 cgaagctacc tacgtgggca cctggagtgg cttagaccgg agcggcggtg ttgtacgaaa
     3721 acagccgcca acccaagccg tggcgaagaa gcaccgtcca gtgacagttg ctgatccccc
     3781 cgaacaccga ccagcccgac accggcagac cgagtagcag tggcgtcaga ccagcgatca
     3841 acccgccgtg ggtacagagc accgcgctgc tgtggccgtg cgcgtcgagc tcggagacca
     3901 cgcgcgcggc ccgtcctgcc acctctaccc gggtctcgcc acctggcggg gcccattccg
     3961 ggttgccgcg ccaggtctgt atgccgcccg ggcaacgcac ctccacctcg gcgtgggtca
     4021 agccctgcca cgtcccc
//
LOCUS       DATADN010000806         1527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_842900, whole genome shotgun sequence.
ACCESSION   DATADN010000806 DATADN010000000
VERSION     DATADN010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842900"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..578)
                     /locus_tag="VHH53_10500"
     CDS             complement(111..578)
                     /locus_tag="VHH53_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2290597.1"
                     /translation="MTVALRRSWAKDLDAATLYELLKLRVEVFVVEQACPYPELDGRD
                     LLAETRHFWLEGPDGEVISTLRLMEEHPGGQKGFRIGRVCTRKDARGHGHTARLMQAA
                     LADVGDHPCRIDAQTYLEAMYARHGFARAGDEFVDDGILHVPMLRPASGTAHP"
     gene            complement(591..>1527)
                     /locus_tag="VHH53_10505"
     CDS             complement(591..>1527)
                     /locus_tag="VHH53_10505"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF003606.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEX2290598.1"
                     /translation="RDLDKQSDGSWSVKVVNRRTGAKRKLNAKFVFVGAGGGALPLLQ
                     KAGIEEAKGFGGFPVGGQWLRSGNPELTARHQAKVYGMPPLGAPPMSVPHLDTRVING
                     KSWLLFGPFAGWSPKFLKQGKVTDLPFSVKPNNLASMLGVGLTEMGLLKYLIGQLLLS
                     EADRVESLRDFAPSARNSDWELDIAGQRVQVIRRKGKGGVLEFGTTVLAAKDGSIAGL
                     LGASPGASTAVPAMLDVMERCFGERYTAWLPKLKEIVPSLGTKLSDEPKLFQEVWAHG
                     TKVLKLDRPGSGLPIAGPDTEHHEPDASRATAVTA"
BASE COUNT          229 a          523 c          532 g          243 t
ORIGIN      
        1 gatcagcacg ccgccgatct cgggacgcac cgcgcacagc agcagggcca gcagcaaccg
       61 gtcgtgaccg acgatcgcgc tgaacggata ggtgggcgtc gcgggcgcgg tcacgggtgg
      121 gccgtcccgg aggccgggcg cagcatcggc acgtgcagga tgccgtcgtc gacgaattcg
      181 tcaccggcac gggcgaatcc gtgccgagcg tacatcgcct ccagataggt ctgtgcatcg
      241 atgcggcacg ggtggtcgcc gacgtcggcg agtgcggcct gcatcaatcg ggcggtgtgg
      301 ccgtgaccgc gggcatcctt gcgggtgcag acccggccaa tgcggaatcc cttctgcccg
      361 ccggggtgtt cctccatcaa ccgcagggtg gagatcacct caccgtcggg accctccagc
      421 cagaagtgac gagtctcggc gagcaggtcc cggccgtcga gttccgggta cgggcaggcc
      481 tgttcgacga cgaacacctc cacccgcagc ttgagcagtt cgtagagcgt tgccgcgtcc
      541 aggtccttgg cccagctgcg gcgcagcgcg accgtcatcg tcgcaccacg tcaggcggtc
      601 accgcggttg cgcgcgaagc gtcgggctca tggtgctcgg tgtcggggcc tgcgatcggc
      661 agacccgagc ccgggcggtc cagcttgagg accttggtgc cgtgcgccca cacctcttgg
      721 aacagcttgg gctcgtcgga gagcttggtg cccagcgacg gcacgatctc cttgagcttc
      781 ggcagccatg cggtgtagcg ctcaccgaag cagcgctcca tgacgtcgag catcgcgggc
      841 accgcggtcg acgcgccggg tgacgccccg agcaggccgg cgatgcttcc gtccttggcg
      901 gccaggaccg tggtgccgaa ctccagcacc ccgcccttgc ccttgcggcg gatcacctgc
      961 acccgctgac cggcgatgtc gagctcccag tcggagttcc gtgcgctggg ggcgaagtcg
     1021 cgcagcgact ccacccggtc ggcctcgctg agcagcagct ggccgatgag gtatttcagc
     1081 aggcccatct cggtgaggcc gacgcccagc atcgacgcga ggttgttcgg cttgaccgag
     1141 aacggcaggt cggtgacctt gccctgtttg aggaacttcg gcgaccagcc cgcgaacggt
     1201 ccgaacaaca gccaggactt cccgttgatg acgcgggtgt cgaggtgggg caccgacatc
     1261 ggcggtgcac caaggggcgg catcccgtag accttggcct ggtgcctggc cgtcagctcg
     1321 ggattgccgc tgcgcagcca ctgcccgccc accgggaagc cgccgaagcc cttggcctcc
     1381 tcgatgccgg ccttctgcag cagcggcagc gcgccgccgc cggcgccgac gaagacgaac
     1441 ttcgcgttga gtttgcgttt ggcgccggtg cggcggttca ccaccttgac cgaccagctg
     1501 ccgtcggact gcttgtccag gtcgcgg
//
LOCUS       DATADN010000807         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1294241, whole genome shotgun sequence.
ACCESSION   DATADN010000807 DATADN010000000
VERSION     DATADN010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294241"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VHH53_10510"
     CDS             complement(<1..476)
                     /locus_tag="VHH53_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2290599.1"
                     /translation="MPRDVFGTDFPFMDHSELLSFEEIERLGTVFARLGVSKLRLTGG
                     EPLLRHGIERLVEQLVNIEGVEDVALTTNGSLLAGKARALRDAGLRRVTVSLDSLDEP
                     TFRAVSDVKIPLARVIKGIAAAADVGMTPVKVNAVIKRGMNDGQGIVDLAGFGREHG"
     gene            complement(645..>1768)
                     /locus_tag="VHH53_10515"
     CDS             complement(645..>1768)
                     /locus_tag="VHH53_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2290600.1"
                     /translation="TVSTTEVLAANCADLAAAEQSGMTQALIDRLRLSDARLTEMAGQ
                     LELLAQVPFPSRDRVLEDRPDGLRLIERRRPVGVVGATFEARPNVTVDVASQLVKSRN
                     AGVLRTGSAALRSALALCEHVIAPALADASLDPGAVQLVPDPRRDCALALVQHRELIP
                     LVIVRGSGDTTRTLAAEGARHGVRVLAHADGGGVLYVDAAADPMLAVKLLVAGLDRLG
                     VCNRVNLMLVADGAPEELHEALLEAARQRGIALSLPPHDHPLGHEWALDTGREATLTV
                     SRVDGPRQAAQVANQETSGLAATIVTNDKAAAHEFMDSYEGTGVFWNASTRLLDGFKL
                     RRVPETGINVDRVPGPRGPVTFADLYLRQFVIIPVALMA"
BASE COUNT          319 a          617 c          542 g          290 t
ORIGIN      
        1 ccgtgctcgc gcccgaagcc ggcgagatcg acaatgccct gcccgtcgtt catgcctctc
       61 ttgatcaccg cgttgacctt gacgggagtc atgccgacgt cggcagcggc cgcgattccc
      121 ttgatcaccc gggccagcgg gattttcaca tcactgacgg cccggaatgt cggctcgtcg
      181 agagagtcca ggctaaccgt gacccgtctc aaaccggcat ctcgaagggc tcgtgccttc
      241 ccggccagca gcgacccgtt ggtcgtcagc gccacgtcct ccacgccttc gatgttgaca
      301 agctgctcga caagtcgctc gatgccatgg cgtagcaacg gctctccacc ggtgagccgc
      361 agcttactga cacccaaccg cgcaaaaacc gtcccgagcc gctcgatctc ctcgaacgac
      421 agcagctccg agtgatccat gaaggggaag tcagtcccga agacgtcgcg cggcatgcag
      481 tagcagcagc ggaagttgca cctatcggtc actgagatcc gcaaatcccg cagtggacgg
      541 ccgaacttgt cgctcacaga ggtcatgggc ttcacacccg atcttcgtgt cgtaccgtaa
      601 aaaagttcag ttcaaccatc taggataccc gtctagggta cccgctaagc catcagtgca
      661 accgggatga tcacaaactg tcgcaggtac aggtcagcaa aggtgacggg tccgcgaggt
      721 ccgggcaccc gatcgacgtt gatcccggtc tcgggaactc ttcgtagctt gaacccatca
      781 agcagacggg tgctggcatt ccagaacacc ccggtgccct cgtagctgtc catgaactcg
      841 tgcgccgcgg ccttgtcgtt ggtgacgatg gtggcggcca gcccggaggt ctcctggttc
      901 gcgacctgag ctgcctgcct tggaccgtcg acccggctga cggtcagcgt tgcctctcgc
      961 ccggtatcca acgcccactc atgccccaac gggtggtcat gcgggggcag cgacaacgcg
     1021 atcccgcgct gccgggcggc ctcaagcagc gcctcgtgta actcttccgg ggcgccatcg
     1081 gccaccagca tcaggttcac ccggttgcag acgcccagcc ggtccagccc agcaaccagc
     1141 agcttgacgg ccagcatggg atcggccgcg gcatcgacgt aaagcacccc gccaccatcg
     1201 gcatgggcca acactcgcac cccgtgacga gccccctcgg cggccagcgt ccgggtggtg
     1261 tctccgctgc cccgcacgat gaccaacggg atcagttcac ggtgctgcac cagtgccagg
     1321 gcgcagtccc ggcgtgggtc cggcaccagt tgcacggcgc cagggtccaa gcttgcatcg
     1381 gccagcgccg gcgcgatgac gtgttcgcac agtgccagcg ccgagcgcag cgccgccgat
     1441 ccggtgcgca gcactccggc gttgcgggat ttcaccagct gcgaggcgac gtcgacggtg
     1501 acattgggcc gggcctcgaa ggtggcgccc accacgccta ccgggcggcg acgctcaatc
     1561 agccgcaaac cgtctgggcg gtcttccagc acccgatccc gcgacgggaa tgggacctgc
     1621 gccagcaact cgagctggcc ggccatctcg gtcagccggg catcgctcag gcgcaagcgg
     1681 tcgatcagcg cctgcgtcat cccggattgc tcagcggcgg cgaggtcagc gcagttcgcg
     1741 gcaagcacct ccgtggtgga gacggtca
//
LOCUS       DATADN010000808         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_84773, whole genome shotgun sequence.
ACCESSION   DATADN010000808 DATADN010000000
VERSION     DATADN010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84773"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..879
                     /locus_tag="VHH53_10520"
     CDS             460..879
                     /locus_tag="VHH53_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX2290601.1"
                     /translation="MRTIGNILWFLLAGWWLALLYAVAGVLAFLLIITIPFGIASFRL
                     AGYSLWPFGRRVVVQRGAGVASVIGNIVWIVLFGWELFFAHIIAGVLLMLTIIGIPFG
                     VASLKLSVLALVPLGTRVVDADTAWALPQYRREPAYR"
     gene            complement(895..>4260)
                     /gene="mfd"
                     /locus_tag="VHH53_10525"
     CDS             complement(895..>4260)
                     /gene="mfd"
                     /locus_tag="VHH53_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX2290602.1"
                     /translation="VVAELPSWETLPHERLSPRADTVGRRLAVLRRLAHPEEGLAGSA
                     GPLLVVVAAVRSLIQPMLGGLGEIAPVRLRVGQVHDFDDVLARLADLAYTRVDMVDRR
                     GEFAVRGGIVDVFPPTADHPLRVEFWGDEVCEIRSFAVADQRTLAPTDELHAPPCREL
                     LLTDAVRATAAQLAAHVPAGPVGELLEKVSSGVPADGMESLIPVLAGTELALLTDLMP
                     KGTHVLLADPERIRARAADLVRTCEEFLEASWMVAAGGGAAPVDVAALNLAEWDVTAS
                     AYRSLGEVAEHARDTGVAWWTLSPLTTDAEGPLRLQIIPTQPYHGDVARAFADLRAHT
                     ATGGAAVLVVPGTGTAQRACEQLRDADVPAVHAADGLVQPPAAGTVTVVRGTLEDGFS
                     TPALGLVVLTETDITGARGGTRTQDMQKMPVRRRNAVDPLALQPGDHVVHEQHGIGRF
                     LEMVQRSVGGATREYLLVEYAASKRGQPGDRLFIPTDQLDQVTRYVGGELPTLNKLGG
                     SDWAKTKGRARKAVKEIAAELVQLYAARQSSPGHAFGPDTPWQAELEDAFPYIETPDQ
                     LAAIDEVKADMRREVPMDRVVCGDVGYGKTEIAVRAAFKAVQDGKQVAVLVPTTLLAQ
                     QHLQTFTDRMRSFPVTVRGLSRFSDAAQASEVLRGLADGSVDIVIGTHRLLQSGVRWK
                     DLGLVVVDEEQRFGVEHKEHIKALRTAVDVLTMSATPIPRTLEMSLAGIREMSTILTP
                     PEERHPVLTYVGAYDDKQVGAAIRRELLRDGQVFYVHNRVSSIEKAARRIRELVPEAR
                     VAVAHGQMNEDRLERTVAGFWEREHDVLVCTTIVETGLDISNANTLIVERGDLLGLAQ
                     LHQLRGRVGRGRERGYAYFLYPPEVPLTETAHDRLSTIAQHTELGAGMAVAMKDLEIR
                     GAGNILGGEQSGHIAGVGFDLYIRLVGEAVEAFRSAAGAAPSGAATEPAPARVELPVD
                     AHLPHDYIPGERLRLEAYRKIAEAVDAAGLAAITDELTDRYGALPPAVVTLLDVARFR
                     QVCRALGITEVTAPGSTLRFAPVHLADSQVVRLGRLYPKAVYKPAVATLSVPRPTEGA
                     DRVGAAPLRDRELLGWCTRLVTAVLGPAPAPAG"
BASE COUNT          673 a         1514 c         1433 g          640 t
ORIGIN      
        1 cagcgcattc ggcgtcgctg cggcgacgag cgcaccacct cctccagcca gttccgggca
       61 cgtacttata gaccgtggtg cgggagactg caagcaactt ggcgatggag ccaaccgtac
      121 tgtcgcgtcg gacagtgcta ccaggacgac ctgcgtgaga tgcaggccca accctgctat
      181 ccgagctcac ccgatccacc ggagtagcga agggttgtag tcaagggaaa cagcacaata
      241 tgtgcctgtg tgcgaacacc accacgtcat cgtgcaccgc caaggatggc acatccaact
      301 cgacgcccgc ggccatccga attcataccc ccaaaggccg tggaacccgc cagaacacca
      361 ctccacgacc cactgcggaa atgacactta cgtcaggtat gacctgaagc cctgtgcgcg
      421 caccaggcgg cagcggcccg gttcaggcat cctggaggca tgcgcacgat cggcaatatc
      481 ttgtggttcc tgctggcagg ctggtggctc gcgctgctgt acgcggtcgc gggggtgctg
      541 gcgttcctgc tgatcatcac tatcccgttc ggcatcgcgt cgttccggct cgccgggtac
      601 tcgttgtggc cgttcggccg ccgggtggtc gtccaacggg gcgcgggcgt ggcgtcggtg
      661 atcggcaaca ttgtgtggat tgtgttgttc ggttgggagc tctttttcgc acacatcatc
      721 gccggggtgc tgctgatgct gacgatcatc ggcatcccgt tcggtgttgc gtcgctgaag
      781 ctgtccgtgc tcgcgctggt gccactgggc acgagggtgg tcgacgccga caccgcgtgg
      841 gccctgccgc agtaccggcg cgagcctgcg taccgctgaa gccgggacgc ggggtcagcc
      901 ggccggggcg ggggccggtc cgagcaccgc ggtgaccagc cgagtgcacc agccgagcag
      961 ctcacggtcg cgcagcggtg cagcgcccac ccgatcggct ccttcggtgg ggcgaggcac
     1021 ggacagggtc gctaccgcag gcttgtagac cgctttggga tacagccggc ccagtcgcac
     1081 cacctgcgag tccgccaagt gcaccggggc gaagcgcagt gtggaacccg gtgcggtgac
     1141 ctccgtgatc ccgagcgccc ggcacacctg gcggaaccga gcgacatcca gcagggtgac
     1201 cacggcaggg ggcagcgcgc cgtagcggtc cgtcagctcg tcggtgatgg cggccagccc
     1261 ggccgcgtcc actgcctcgg cgatcttgcg ataggcctcc aggcgcagcc gctcgccggg
     1321 gatgtagtcg tgcggcaggt gcgcgtccac cggtagctcc acccgcgccg gcgcaggctc
     1381 ggtggcagca ccagagggtg ccgcaccggc cgcgctgcgg aatgcctcca cggcctcgcc
     1441 gaccagccgg atatagaggt cgaagccgac cccggcgatg tgcccggact gctcgccgcc
     1501 caggatgttg ccggcaccgc ggatctccag gtccttcatc gcgacggcca tgccggcgcc
     1561 gagctcggtg tgctgggcga tggtggacag ccggtcgtgc gccgtctcgg tcagcggaac
     1621 ctcgggcggg tagaggaagt aggcataccc acgctcgcgg ccgcgcccca cccggccacg
     1681 tagctggtgc aactgggcga gcccgagcag gtcaccgcgc tccacgatca gcgtattggc
     1741 gttggagatg tccagcccgg tctcgacgat cgtggtgcac accagcacgt catgctcgcg
     1801 ttcccagaac ccagcgacgg tacgttctag ccggtcctcg ttcatctgcc cgtgcgccac
     1861 cgccacccgc gcctcaggta ccagctcgcg gatccgccgg gcggccttct cgatcgacga
     1921 cacccggttg tgcacgtaga agacctgccc atcgcgcagc agctcacgac ggatcgcggc
     1981 accgacctgc ttgtcgtcgt aggccccgac gtaggtcagc accgggtggc gctcctcggg
     2041 cggggtcagg atggtcgaca tctcacggat ccccgccagg ctcatctcca gggtccgcgg
     2101 gatcggggtt gcggacatgg tgagcacgtc gacggcggtg cgcaaggcct tgatgtgctc
     2161 cttgtgctcg acaccgaagc gctgctcctc gtcgaccacc accagcccta gatccttcca
     2221 gcgcacccct gactgcagca gccggtgggt gccgatcacg atatctaccg aaccgtcggc
     2281 cagcccgcgc aatacttcgg acgcctgcgc cgcgtcgcta aaccgggaca gcccgcgcac
     2341 cgtgaccggg aacgaacgca tccggtcggt gaaggtctgc aggtgctgct gggccagcag
     2401 cgtggtgggg accagcacgg ccacctgctt gccgtcctgc accgccttga aggcggcccg
     2461 cacggcgatc tcggtcttgc cgtagccgac atcgccacag accacacggt ccatcgggac
     2521 ctcgcggcgc atgtccgcct tgacctcgtc gatcgcggcc agctggtcgg gcgtctcgat
     2581 gtaggggaag gcgtcctcaa gctcggcctg ccatggggtg tccggaccga aggcgtgccc
     2641 cggcgaggac tgccgagccg catagagctg caccagctcg gcagcgatct ccttgaccgc
     2701 cttgcgggcc cggcccttgg tcttggccca gtccgagcca cccagcttgt tcagggtcgg
     2761 cagctcaccg ccgacgtagc gggtcacctg gtcgagctgg tcggtgggga tgaacaaccg
     2821 gtcccccggc tggccgcgct tgctggctgc gtactccacc agcaggtact cccgagttgc
     2881 cccgccgacg gaacgttgca ccatctccag gaaccggcca atgccgtgtt gctcgtgcac
     2941 cacatgatcg cctggctgca gcgccaacgg atcgacggcg ttgcggcggc ggacaggcat
     3001 cttctgcatg tcctgggtgc gggtcccgcc acgggcaccc gtgatgtcgg tctcggtgag
     3061 caccaccaac cccagcgctg gggtcgaaaa gccgtcctcc agagtcccgc ggaccacagt
     3121 gacggtgccc gcggctggtg gctgcacaag cccgtcggcg gcgtgcacgg cgggtacatc
     3181 ggcgtcgcgc aactgctcgc aggcccgctg cgccgtgccg gtgccgggga ccacgagcac
     3241 cgccgcacca ccggtggcgg tatgggcgcg caagtcggcg aaagctcgcg cgacgtcacc
     3301 gtggtagggc tgcgtcggaa tgatctgcaa ccgcagcggg ccctcggcgt cagtggtcag
     3361 cgggctcagc gtccaccagg ccaccccggt gtcgcgggcg tgctcagcaa cctcgcccag
     3421 gctgcggtag gccgaggcgg tgacatccca ctccgctagg ttcagcgccg cgacgtcgac
     3481 cggggcggca ccgccgcccg cggccaccat ccaggacgcc tccaggaact cctcacaggt
     3541 gcgcacaaga tccgcggccc gggcccggat ccgctccgga tcagccagca gcacatgggt
     3601 gcccttgggc atcaggtcgg tgagcagcgc aagctcagtg cctgccagca ctgggatgag
     3661 cgactccata ccgtcggccg gcaccccgga ggaaaccttc tccagcagct caccgaccgg
     3721 cccggcaggc acgtgcgcgg cgagctgtgc cgcggtcgcc cgcaccgcat cggtcagcag
     3781 cagttcccgg cacggcggtg cgtgcagctc atcggtgggc gccagggtgc gctggtccgc
     3841 gacagcgaac gagcggatct cgcagacctc gtcaccccag aactccaccc gcagcggatg
     3901 gtcggcagtg ggcgggaaga cgtcgacgat gccgccccgc accgcgaact caccgcgccg
     3961 gtccaccatg tcaacccggg tgtaagcgag gtcggccagc cgggccagca cgtcgtcgaa
     4021 gtcgtgcacc tgcccgaccc gcaaccgcac cggcgcgatc tcgccgagcc cgccgagcat
     4081 cggctggatc agcgaccgca cggcggccac caccaccagc agcggcccgg cagaaccggc
     4141 aaggccttct tccgggtggg ccagccggcg cagcacggcc aaccggcgcc ccacagtgtc
     4201 ggctcggggc gacagccgct cgtgtggcag cgtctcccag gacggcagct cggcgaccac
//
LOCUS       DATADN010000809         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_85024, whole genome shotgun sequence.
ACCESSION   DATADN010000809 DATADN010000000
VERSION     DATADN010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85024"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VHH53_10530"
     CDS             <1..291
                     /locus_tag="VHH53_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290603.1"
                     /translation="AASAVHIADRRTMALLLATILLTHVEFPFWFFDLPSGEPVSRAV
                     LTARNVCLITLAFCAVRAWLAASGTQSAGGSHERELPAAMPRSLASERPDHR"
     gene            573..863
                     /locus_tag="VHH53_10535"
     CDS             573..863
                     /locus_tag="VHH53_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290604.1"
                     /translation="MVEGQAGVGQERPDQLLVITDPLIPCSAASATSMTVSPAKLANS
                     TRTGYQQIDRWHSYLVRHEDSDGLPVLNPACARTVTGHGRLGFIQCNSLSFI"
     gene            complement(918..>1620)
                     /locus_tag="VHH53_10540"
     CDS             complement(918..>1620)
                     /locus_tag="VHH53_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290605.1"
                     /translation="TGLVIFAVVLYGGVPDEVGTLSYIGNRLGLYVVLFVLLWLAVQP
                     SSRRMRWLACGTALAVAFGLVLVRFPVEGRYNTHVHEFMSARQVLRPGSTLVTLRFWV
                     DTPPFARFPDQDPLLHITSRLAVESKGVDINHLDAWYSYFPVQFRREYNLRHRFGPHF
                     NELKSVSEWSELLKDDRTGRIHIDYVLLWGTRRPDQRVREDPRLVEVMDQLTQRYRLT
                     YVSEPLGLVEVYEHR"
BASE COUNT          304 a          531 c          488 g          297 t
ORIGIN      
        1 gcggcctcgg cggtccacat cgcggaccgt cgaaccatgg ctctcctgct cgccaccatc
       61 ctgctgaccc acgtcgagtt ccccttctgg ttcttcgacc taccctctgg cgagccggtg
      121 agccgcgcgg tgctcaccgc gaggaacgtc tgtctcatca cgctggcgtt ctgcgccgtg
      181 cgggcgtggc tggccgcgtc cggcacgcag agcgccggcg ggtcccacga gcgagagctg
      241 cccgccgcga tgccgcggtc tctcgccagc gaacgcccgg atcatcgttg aatccggatc
      301 cgccgccggt ccgcggctgc ccgatccacg gcggctgcca tgccgttggg gtggagcatg
      361 ccctgacggt gttgtcggcg tagccatcgg cccgcagcgc cagctccccg acaccgtgag
      421 cacgcgaagt atggagcgct atgcttcaag cctccggacg cactacagat gcatctgccc
      481 tgcttaggaa gtctgttagc tgcccggcgt tggtcaccgc ctgatcgcgg agcagttgcc
      541 tatttgagct ttcataagtt gggtggaggt tggtggtcga gggtcaggcc ggtgtgggcc
      601 aggaacgacc agaccagctg ctggtcatca cagatccact gatcccctgc tcggcggcgt
      661 cggccacctc gatgacggtg tcgccggcga agttggccaa ctccacgcgg accggttacc
      721 aacagatcga ccgctggcac tcttacctgg ttcgccacga ggactctgat gggctacccg
      781 tgctgaatcc agcatgcgcc cgtaccgtca cgggccacgg ccgattgggg ttcatccagt
      841 gcaactccct gagcttcatc tgaagacgac agcgttcctc tgcttcaacg aggtcccgtc
      901 agttagccca tgtggtctca gcggtgctca tatacctcga ccaatcctag aggctccgag
      961 acataggtaa ggcgataacg ctgggtgagc tgatccatca cctcgacgag gcgtgggtct
     1021 tcacgcaccc gctggtcagg gcggcgcgtt ccccacagca acacgtagtc gatgtggatg
     1081 cggcccgtcc ggtcgtcctt gagcagctcg gaccactccg acaccgactt tagttcattg
     1141 aagtgcgggc cgaatcggtg tcggaggttg tattcacgcc ggaattgtac cgggaaatag
     1201 ctgtaccagg cgtccaggtg gtttatgtcc acgcccttgc tctccaccgc cagccgactt
     1261 gttatatgca gcagcggatc ctggtcagga aaacgggcga acggtggggt gtcaacccag
     1321 aagcgcagcg tcacgagcgt tgaaccgggc cgcagcacct gccgggcact catgaactca
     1381 tgaacgtgcg tgttgtaacg accttcgacc ggaaaccgca ccaggacaag gccgaatgcg
     1441 actgcgagcg ccgtcccgca ggcaagccaa cgcatacgcc gcgaactcgg ctgcacggct
     1501 aaccacagca gaacaaacag cactacatat aaaccaagcc gattaccgat atagctcaag
     1561 gtgccgacct cgtctgggac cccgccgtac aggacaacgg caaagatcac gagaccggtc
//
LOCUS       DATADN010000810         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_410562, whole genome shotgun sequence.
ACCESSION   DATADN010000810 DATADN010000000
VERSION     DATADN010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410562"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..1618
                     /locus_tag="VHH53_10545"
                     /pseudo
     CDS             471..1618
                     /locus_tag="VHH53_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          251 a          535 c          583 g          256 t
ORIGIN      
        1 gccgttgatg gggccggcgg gggtgatcgt cctcgacgcc tcggcgttgc gagcactcat
       61 gaccacgacc cggatgggat gtcgtggccg cgcagccgcc ggcggggacg ccagcgcggt
      121 caactacctt tgatcgacct ggggtgccgc tgcgcgattg cggcagatca gcaccggcca
      181 agcaggcctt gggccggccg ggaccgcgat cagacgcgct cagagctgcg tctccgggtc
      241 acctaggcct atgtgctggc gggtagccag tgagtttgtg atgcgtgcct gcgacacttg
      301 gcgtcgagcc aaggccttta tgcccactga cctggggctt tgctcccccg atcggactcg
      361 aacaatagtc ctgctgaacc gtcccggcgg gtccggagac tctggtccgc cccgagttca
      421 gtggagtcgg gtaactggaa tcctggccgc gcaaccccgg aggagggttc gtgggcaggc
      481 gaggttatcc gccggagttc cggcgcaagg ttctggacct ggtcgagtcc ggtcggccgg
      541 tggtcgacgt ggcccgggat ctggggatca gcgcgcagtc gatctacgtc tggcgccgac
      601 aggaccggat cgacaagggc ctcgagcccg gactgaacac cgcggagaag tcggagctga
      661 ccgcggccaa gcggcggatc gccgagctgg aggccgagct ggccgttcat cgccgggcca
      721 gcgagctgtt ggggaaggtg gtgcccccaa gaggcggttc gaggccatct ccgtgatggc
      781 cggcgaaggt cactcggtgc agctggccgc ccgggtgctt caggtctcgg agtcgggcta
      841 ctacgacgcg ctgacccgcc cgccctcggc ccggatgatc cggcatgcct ggctgaccga
      901 ccagatccgc gaggtccaca ccgcctccaa cggcacctac ggggcacggc gggtgcatgc
      961 cgagctcacc ttgggccgag gcctggtggt cgcgcacggc accgtggaga tgctgatgag
     1021 ccgggcgggc attcgcgggg tcaccggccg gccgcgctgg cggtcgtctc ggccggacct
     1081 ggtctcgacc gacctggtgg aacgcaactt cgcccgcacc gcgccgaacc agctgtgggt
     1141 caccgacatc accgagcacc ccacccggga gggcaaggtc tactgcgcgg tggtgctcga
     1201 cacctactcc cggagggtgg tgggctggtc gatcgactcg gcacccaccg cggcgctggt
     1261 caccaacgcg ctggggatgg cgatcgactc gcgcaccccg ccgtcgggag cggtgatcca
     1321 ctccgaccgg ggcgtgcagt tcggctcctg ggcgttcacc gaccgggcca aggcctcggg
     1381 gctgctgccg tcgatgggca gcatcgccga ctgctacgac aacgcgatga tcgagtcgtt
     1441 ctgggcccgg atgcaggtcg agctgctcga ccgccggcga tggaacaccc ggatcgagct
     1501 ggccaacgcg atgttcgagt acctcgagat ctggcacaac cggcgccgtc ggcacagcgc
     1561 gctgggctgg ctgtcgcccg tagagttcga aacccaaaac aagatcatcg tggcctagag
     1621 attcc
//
LOCUS       DATADN010000811         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_410593, whole genome shotgun sequence.
ACCESSION   DATADN010000811 DATADN010000000
VERSION     DATADN010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410593"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VHH53_10550"
     CDS             complement(<1..311)
                     /locus_tag="VHH53_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290606.1"
                     /translation="MGGAERGEAKRKQQATQAGTATRGSGGPSRGVVAAVAIVVALAL
                     VVGIGLWLQNRHQPDDLPAAIPVAAAGPEYPVALQGDTVVAGSPEAPVTIDIYEDFLC
                     PG"
     gene            509..1219
                     /locus_tag="VHH53_10555"
     CDS             509..1219
                     /locus_tag="VHH53_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX2290607.1"
                     /translation="MAVEPGDEVGHVHQDLSGGWLRPAVFGAMDGLVTNIALVAGVGG
                     GGAGADLIVLTGMAGLVAGAFSMALGEYASVETQNDAVRAEITVERDELRRHPAAEKT
                     ELITSFMQMGLTRRTAQQVADEVHADPSLAVRVHINQELGVNPDEQPSPWVAGGSSFL
                     CFAIGALIPLIPYLLGFDSLAAGLGAGAVGLFVAGALASRFTIRSWWLGGLRQVGFGA
                     IAAVATYLVGALIGVSMS"
     gene            complement(1226..>1602)
                     /locus_tag="VHH53_10560"
     CDS             complement(1226..>1602)
                     /locus_tag="VHH53_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2290608.1"
                     /translation="AVVAGIGTGLITPAQGATVADVIGSRAKGGPVLAAFQMTADIGA
                     ILGPIAAGLLADQLSYAAAFTLTGALSLLAAVAWLRAPETLPRAQDSTATDISAECGC
                     RDEGPEVPTGQRMAGRPRAPEA"
BASE COUNT          226 a          534 c          587 g          255 t
ORIGIN      
        1 cccgggcaca ggaagtcctc gtagatatcg atggttaccg gcgcctcggg gcttccggcc
       61 accacggtgt cgccctgcag cgccaccggg tactccgggc cggcggcggc aaccgggatc
      121 gcggctggaa ggtcatcggg ctggtggcgg ttctgcagcc agagcccgat cccgacgaca
      181 agcgcgagcg caacgacgat ggcgactgcg gccactaccc cgcggctggg cccgccactc
      241 ccgcgggtcg ccgtccccgc ctgggtggcc tgctgcttgc gctttgcctc gccccgttcg
      301 gcgccgccca caacgcgtcc tcccagctat ccggccagat acccggctcg attgcactgc
      361 ctgtccgaat ctgcgcgcag ccatccgtcg agactcaccc gatcagcgta gccatacagg
      421 tgaggctgcc catgccgagc cgaacctgag tgtcgggtgg agctggtgtg ctccgtactg
      481 tgattcggcg gcgtcacgga ggtgtgggat ggctgtcgag ccaggtgacg aggtcgggca
      541 cgtgcaccag gacttatctg gaggatggct acgaccggcc gttttcggtg cgatggacgg
      601 tttggtgacc aacatcgcgc tggtcgccgg ggtgggcggc ggcggggcag gtgccgacct
      661 gatcgtgttg accgggatgg ccgggctggt ggcgggcgcg ttctcgatgg cgctgggcga
      721 gtatgcatcg gtcgagacgc agaacgacgc tgtccgcgcc gagatcacgg tggaacgcga
      781 cgagctgcgc cggcacccgg ccgcggagaa gaccgagctg atcacctcct tcatgcagat
      841 ggggctgacc cgcaggaccg cgcagcaggt ggccgacgag gtgcacgccg acccgtcgct
      901 ggcggtccgg gtgcacatca accaggagct cggggtgaat cccgacgagc agccctcccc
      961 ctgggtggcc ggtggctcgt cattcctgtg cttcgcgatc ggcgccctga tcccactgat
     1021 cccctacctg ctcggcttcg actcgctggc ggcaggcctc ggggcagggg cggttggatt
     1081 gttcgtggcc ggggcgctcg cgtcacggtt caccatccgc tcgtggtggc tgggcgggct
     1141 gcgccaggtg gggttcggcg cgatcgccgc ggtagcgacc tacttggtcg gcgcgctgat
     1201 cggggtcagc atgagctgag cggcgtcacg cctccggcgc gcgcggccgc ccagccatcc
     1261 gctgcccggt tggtacctcg gggccttcgt cgcggcaacc gcattcggcg gagatgtcgg
     1321 ttgcggtgct gtcctgcgcc cggggcagtg tctccggtgc gcgcagccag gccaccgcgg
     1381 caagcagcga gagggcaccg gtcaaggtga aggccgcggc gtaggaaagc tggtcggcga
     1441 gcagcccggc ggcgatcgga ccgaggattg caccgatatc ggcagtcatc tgaaaggcgg
     1501 cgagcaccgg accgcccttg gcccgcgaac cgatcacatc ggccaccgtg gcgccctgtg
     1561 ccggcgtgat cagcccggta ccgataccag cgacgacagc gg
//
LOCUS       DATADN010000812         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_557239, whole genome shotgun sequence.
ACCESSION   DATADN010000812 DATADN010000000
VERSION     DATADN010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557239"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..620
                     /locus_tag="VHH53_10565"
     CDS             327..620
                     /locus_tag="VHH53_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2290609.1"
                     /translation="MTDTRRLPKPVASEWDWQLRGSCQGLNSSVFFHPDGERGSARAR
                     RADRAKAICQRCPVLEQCRRHALAVREPYGVWGGLTEEERRELWVGRQPLLAG"
     gene            995..1459
                     /locus_tag="VHH53_10570"
     CDS             995..1459
                     /locus_tag="VHH53_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290610.1"
                     /translation="MALIGSGKQAGASTPDEGARQNRRTIVMQVAAEASELPPLRLVA
                     ADLAARADFDLDTVEDLRLAVDEAAAELVAVAAADAVLTCTLWLDAEQMEVTASVPAK
                     PGAWVRQDSFGWRVLTTLVDEVRVTGEPDSDCPVPSITLIKRCHDETVAQRW"
BASE COUNT          291 a          498 c          543 g          263 t
ORIGIN      
        1 gtcgaaggca cgcggaggaa atcctctcct tgagggatgg tggcggctga tgcgtgccac
       61 ccctcaatct cagacacccg agcctgcagg cgcggcagtc cagatgcgga gaagctcgtg
      121 cgctatgtaa ggcgcgtcgc agtcgacctc ggtcacacca gcatcggcgt aaccatcgca
      181 gtccattatg cactatgtga tcgataccct gcgccagccc ccgctaccag ccgtaagtgg
      241 gcgctgagca ggtttgccac gctcacgcca tgggtaatcg ttacttgacg tggggagctg
      301 gccaccggct gaggatggtg tgggagatga cagatacccg acgacttccc aagccggtag
      361 cgagcgagtg ggattggcag cttcgaggat cctgccaagg cttgaatagc agcgtctttt
      421 tccatccaga cggtgagcga gggtcagcgc gggcccgccg cgctgaccgc gccaaggcga
      481 tatgtcagcg ttgccccgtc ctggagcagt gccgccgcca cgcgctggcc gtgcgggagc
      541 cctacggggt gtggggaggg ctgaccgagg aggaacgccg cgagttgtgg gtgggtcggc
      601 agccgctgct ggccggttaa taacaccagc cctcgcaccg ctcgctgagc ttcctgaaca
      661 ctcagcgcta cctgcgtagt gattgtgcgc gtcgtgaact cgacagcacc gacgtcggcg
      721 gccgcggaga tgccggtcac gtcgagttca cgacgcacca gccccgtcca gagccgaagg
      781 cacacccgga gcactgagcc actgcataca tgacctaccg tcacgtaata cccgccggac
      841 gtccggcgcc ggcccctaag ccgtagcgca gcctggtgcc gtggacgcgc gacagtcttg
      901 ggtgtgcgca aacttttcag tcggagtagt gcgttctcct acggacgcca ggcaggatcg
      961 gccccgggca ggttcatcgt cggtaggagg cgtcgtggca ttgatcgggt ccggaaagca
     1021 ggcaggcgcc agcacgcccg acgaaggtgc acggcaaaac cggcgtacca tcgtgatgca
     1081 ggtcgcagcc gaggcatcgg agctgcctcc actgcgcctg gtcgcggccg accttgccgc
     1141 ccgggctgat ttcgatctcg acaccgtaga agatttgcgc ctggcggtgg acgaggcagc
     1201 cgcggagctg gtggctgtcg ctgctgcgga tgcggtgctt acctgcacgt tgtggctcga
     1261 cgccgagcag atggaagtca ccgcgtcggt gccggcgaaa ccgggggcct gggtgcggca
     1321 ggattccttc ggctggcggg tgctgaccac gctggtcgac gaagtgcgag tgactggcga
     1381 gccggactcc gactgcccgg taccgagcat cacgctgatc aagcgttgcc acgacgagac
     1441 ggtggcgcag cgttggtgac gcccaccgaa aaatcagagg aatgggccgc gactgagaac
     1501 accgactacg ggcacctagc cgccctgttc gccgagctcg tcgagctgaa tccagacagc
     1561 ccacggcggg atcaactacg tgaccggctg gtgac
//
LOCUS       DATADN010000813         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_703889, whole genome shotgun sequence.
ACCESSION   DATADN010000813 DATADN010000000
VERSION     DATADN010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703889"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..605)
                     /locus_tag="VHH53_10575"
     CDS             complement(99..605)
                     /locus_tag="VHH53_10575"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX2290611.1"
                     /translation="MRDTLVLNASFEPLSTVTLNRAVVLVLQDKAVVEQAHPELRMRG
                     AAVDIPAPRVIRLCQYVRVPFRRRAPWSRRGVLVRDRHRCAYCGRRATTVDHVVPRSQ
                     GGQDTWLNTVASCAQDNHRKANRTPEEAGMPLLRLPFEPSPADAMLLSLGNEDFGALP
                     EWLAQPAA"
     gene            complement(816..1355)
                     /locus_tag="VHH53_10580"
     CDS             complement(816..1355)
                     /locus_tag="VHH53_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX2290612.1"
                     /translation="MTELKRRPLPHDFHPPVPSFTVTSKDIEEGSTLQDAQVHAAGNT
                     SPQLRWEGFPPETKSFAVTCYDPDAPTGSGFWHWVLFDIPASVTELPTGAGSGKFEGL
                     PEGAIHARNDYGDKEFGGAAPPPGDGPHRYVFTVYAVDQEKLGPDAEASPAVVGFNLR
                     FHTLGRAQLIGEYEVPAEG"
     gene            complement(1410..2192)
                     /locus_tag="VHH53_10585"
     CDS             complement(1410..2192)
                     /locus_tag="VHH53_10585"
                     /inference="COORDINATES: protein motif:HMM:NF018738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HEX2290613.1"
                     /translation="MAFKKLLASLGAGGASVETVLHEVNVVPGGVVQGEVRIQRGSVN
                     QQIEGLSVGLQAKVEVESGDQEYKQDIEFTKSRLGGAFELQANAVHAVQFGLEIPWET
                     PITTIDGQELRSMHIGVSTELEIARAVDSGDLDPINVHPLPAQKAILDAFIQLGFRFK
                     NADMERGVIRGTRQKLPFYQEIEFYPPQQYRGLNQVELSFVADQHAMDVVLEMDKKPG
                     LFSEGSDTFRSFQVGLNDYQGTDWTAYLNQWLSEVGSKRNWF"
BASE COUNT          385 a          932 c          731 g          396 t
ORIGIN      
        1 gaagccgtcc atcgactgac acgacgccgg catcaacatg aacgacgggc ccgcttcccc
       61 cgaggaagcg ggcccgtcgc atggcatccg ggccggagct aggccgcggg ctgagccagc
      121 cactccggca gcgccccgaa gtcctcgttg cccagcgaca gcagcatcgc gtccgccgga
      181 ctcggctcga acggcagccg caacagcggc atacccgcct cctccggagt ccggttcgcc
      241 ttgcggtggt tgtcctgcgc gcacgaggcc accgtgttca accaggtgtc ctgaccgccc
      301 tgcgaccgcg gcaccacgtg gtccacggtc gtcgcccgcc tcccgcagta cgcgcaccgg
      361 tgccggtccc ggaccagcac accccgcctc gaccacggcg ctcgtcttcg gaaaggcacc
      421 cgtacgtact ggcagagcct gatcacacgg ggcgccggta tgtcgaccgc ggctccccgc
      481 atccgcagtt cggggtgggc ctgctcgacg acggccttgt cctgaagcac cagaacgacg
      541 gctcggttca gcgtcaccgt cgacagcggc tcgaagctcg cgttcagtac cagcgtgtcc
      601 cgcataccag cccacctccc gtgtgcaccg gcccaccccc tggcgggctg ggatcaactc
      661 tggccgggca cgccgagatg gacaacgcaa tatctgctgc tcccacgggg ggtgaccccc
      721 gcgacccccg gcaacaaaaa tgcccgctcc tgatcaattc caagaccagg agcgggcaaa
      781 cgttccgtga acgccgggat cgcggggcgg ctcactcagc cttcggcggg cacctcgtac
      841 tcaccgatca gctgggcccg cccgagcgtg tggaaccgca ggttgaagcc cacgacggcc
      901 ggcgaagcct ccgcgtccgg accgagcttc tcctggtcca cggcgtacac cgtgaacacg
      961 taccggtgcg ggccgtcccc aggcggcggc gcggcaccgc cgaactcctt gtcgccgtag
     1021 tcgttacgag cgtggatcgc accctcgggc agaccctcga acttgccgct gcccgcaccc
     1081 gtcggcagct ccgtcaccga cgccgggatg tcgaacagca cccagtgcca gaacccgctc
     1141 cccgtcgggg catcggggtc gtagcaggtc acggcgaagc tcttggtctc gggcgggaag
     1201 ccctcccacc gcagctgcgg cgaggtgttg ccggccgcgt ggacctgagc gtcctggagc
     1261 gtcgaaccct cctcgatgtc cttgctcgtc accgtgaacg acggcacggg cggatggaag
     1321 tcgtggggga gcggccgccg cttgagctcg gtcacctcgg tacctcctgg atcggttctt
     1381 gcagttgatc ccgagcctag ggggtgtccc tagaaccagt tgcgcttgct gccgacctcg
     1441 gagagccact ggttgaggta cgccgtccag tcggtcccct gatagtcgtt gagccccacc
     1501 tggaacgaac ggaaggtgtc actgccctcg ctgaacagtc ccggcttctt gtccatttcg
     1561 agcaccacgt ccatggcgtg ctggtcggcc acgaagctca gctcgacctg gttcagcccc
     1621 cggtactgct gcggcgggta gaactcgatc tcctggtaga acggcagctt ctgccgcgta
     1681 ccccggatca caccgcgctc catgtccgcg ttcttgaacc ggaagcccag ctggatgaac
     1741 gcgtccagga tcgccttctg cgccggcagc gggtgcacgt tgatcgggtc caggtcaccg
     1801 gagtccaccg cccgcgcgat ctccagctcc gtggagaccc cgatgtgcat cgagcgcagc
     1861 tcctgcccgt cgatcgtcgt gatcggcgtc tcccacggaa tctccagccc gaactggacc
     1921 gcgtgcactg cgttcgcctg aagctcgaac gccccgccca gccgggactt cgtgaactcg
     1981 atgtcctgct tgtactcctg gtcgccgctc tccacctcga ccttggcctg gagcccgacc
     2041 gagagcccct cgatctgctg gttcacggac ccgcgctgaa tccgcacctc accctggacg
     2101 acaccgcccg gcacgacgtt gacctcgtgc agcaccgtct cgacggaagc cccgccggcc
     2161 cccaggctcg cgagcagctt cttgaacgcc atgtctctcc ctctcgccca ctacgggtgg
     2221 atccttgatc catacaaacg cgatccggcc gtggccggtt ccatcccctc agcctggcaa
     2281 ggccggcgcc ctgaggccac cccaccgcac cacccgtctc cagtaccctc ggagggcatg
     2341 atcgcgaccc ccgaccgtac gcccctgtcc agggagttct tcgaccgacc cgtcctcgat
     2401 gtcgcccccg acctcctcgg ccgcatcctg gtacgcacca cccc
//
LOCUS       DATADN010000814         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_241954, whole genome shotgun sequence.
ACCESSION   DATADN010000814 DATADN010000000
VERSION     DATADN010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241954"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VHH53_10590"
     CDS             <1..742
                     /locus_tag="VHH53_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290614.1"
                     /translation="LAERSNQIVGLVHNTNALLAELQNQSAAIDQISGNISALSQQLH
                     GFIGENRETMKPALDKLNGVLTILDNRKERLQRSLKLLTQYSMSLGESVASGPFFKTY
                     VANLLPGQFLQPFVDAAFSDLGLDPNVLLPSERSDPQVGQPGTPALPVPFPRTGQGGE
                     PRLTVPDAITGNPGDQPCGLPGLPLPGPGCYPYRQPPPAPPPGGPPPGPPAEAPPGLQ
                     STPEPTPAPVMVPAPGGVSHSAPAEAGQ"
     gene            739..2061
                     /locus_tag="VHH53_10595"
     CDS             739..2061
                     /locus_tag="VHH53_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Mce family protein"
                     /protein_id="HEX2290615.1"
                     /translation="MTRTARNILAVALVVILAGGAILLLRNVERIDRTHVVAYFENAN
                     GIYPGDDVRILGVGVGRIESIEPQPERVKISFWYDSKYKVPADAKAAILSPTLVTARA
                     IQLTPPYTGGPVMGDDAVIPQSRTAVPVEWDEVRQQLEKLAETLQPTEPGGVSPLGSV
                     INTTADNLRGEGANIRDTVIKLSQAVTALGDHSTDIFSTVKNLAILVSALQDSTDVMR
                     QLNQNLATVTGLLANDPDEVANAVRDLNNVVGEVQTFVADNRETLGTTSDKLAGVTQA
                     LTDSLADVKQFLHVAPNTLQNYVNIWQPAQGAVSSVPMINNFANPISFLCGAIQAASR
                     LGAEQSAKLCVQYMAPIFKNRQYNFLPVAQNVFVGASTRPNELTYSEDWMRPDYIPPQ
                     PAAPPPPSAPVGSPAPPPPGPPLAAEVPVATNPADGLHGMMVPPGVGP"
     gene            2058..>2778
                     /locus_tag="VHH53_10600"
     CDS             2058..>2778
                     /locus_tag="VHH53_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX2290616.1"
                     /translation="MMRRKLFGVVVIVAAVATGCGWRGLNSVPLPGVEGVGPGAFTVQ
                     AQMPDVDNIEQNSRVRVGDVNVGTVTKLERQGWHALVTMKLNSDVELPANATAKVGQT
                     SLLGSQHIELAPPVDAPPEGRLRDGSLIPLASAGAYPTTEQALAAIALLLNGGGFGNI
                     HDITQALSTAFAGRENDLRSLIEQLDKAIGYLDDQKGDIIAASESLNNLMGQIAEQKP
                     VVDKALRTIPDALAVLRDQRQN"
BASE COUNT          515 a          977 c          868 g          418 t
ORIGIN      
        1 attggccgag cgcagcaacc agatcgtcgg cttggtccac aacaccaacg cgttactggc
       61 cgagctgcag aaccaaagcg ccgcaatcga tcagatctcc ggcaacatct ccgccctcag
      121 ccagcagctg cacggcttca tcggtgagaa ccgcgaaacg atgaagcccg cactggacaa
      181 gctcaacggg gtactcacca tcctcgacaa ccgcaaggag cgcctgcaga ggtcgctcaa
      241 actgcttacc cagtactcga tgtcgctcgg cgaatcggtg gcgtccggcc cattcttcaa
      301 gacctacgtc gccaacctgc tgcccggcca atttttgcag cccttcgtcg acgccgcttt
      361 ctccgacctg gggctggatc ccaatgtgct actgccctcg gaacgcagcg acccacaggt
      421 cggtcagccc ggcacaccgg cgctgccggt ccccttcccg cggacgggcc agggcggcga
      481 accgcggctg accgttcccg acgccatcac cggcaatccc ggcgaccagc cgtgcgggtt
      541 gcccggcttg ccgttgcccg gccccggctg ctacccgtac cgccagccgc cgccggcgcc
      601 gccgccagga gggccgccgc cgggcccgcc cgccgaggcg ccgccgggcc tgcagtcgac
      661 tccggagccg actcccgcac cggtgatggt gcccgctccc ggcggggtct cccactctgc
      721 acccgcggag gccggacagt gacgcgcacc gcgagaaaca tcttggccgt cgcgttggtc
      781 gtgatactgg ccggcggcgc aatcctgctg ttgcgcaacg tcgaacggat cgaccgcacc
      841 cacgtcgtcg catacttcga gaacgccaac ggcatctatc ccggtgacga tgtccgaatt
      901 ctcggggtgg gcgtgggcag gatcgagtcg atcgagccgc agcccgagcg ggtcaagatc
      961 tcgttctggt acgacagcaa gtacaaggtc ccggccgacg ccaaggccgc gatcctgtca
     1021 ccgaccttgg tgaccgcgcg cgccattcaa ctgaccccgc cctacaccgg cggaccggtg
     1081 atgggcgacg acgcggtgat tccgcagtcg cgcaccgccg ttccggtgga gtgggacgag
     1141 gtccgccaac agttggagaa gctggccgag acgctgcagc ccaccgagcc gggcggagtg
     1201 agcccgctgg gctcggtcat caacaccacc gccgacaacc tgcgtggtga gggtgccaac
     1261 atccgcgaca ccgtcatcaa gctttcgcag gcggtgacgg cgctgggcga ccacagcacc
     1321 gacatcttct ccaccgtcaa gaatctggcg atcctggtgt cggcgctgca ggacagcacc
     1381 gacgtgatgc gccagctcaa ccagaacctg gccaccgtga ccggcctgct cgccaacgat
     1441 cccgacgaag tcgccaacgc ggtccgcgac ctcaacaacg tcgtcggaga ggtccagacc
     1501 ttcgtggcgg acaaccgcga aacgctgggc accacatcgg acaagttggc aggggtgacc
     1561 caggcgttga ccgacagcct cgccgacgtc aagcagttcc tgcacgtggc gcccaacacg
     1621 ttgcagaact acgtgaacat ctggcaaccc gcccaaggcg cggtgagcag cgtgccgatg
     1681 atcaataact tcgccaatcc gatttcgttc ctatgcggcg caatccaggc ggcgtcacgg
     1741 ttgggcgccg agcagtcggc gaagttgtgc gtgcagtaca tggcgccgat cttcaagaat
     1801 cggcagtaca acttcctgcc cgtggcccag aacgtgttcg tcggagcgtc gactcgaccg
     1861 aatgagctga cctacagcga ggattggatg cggcccgact acattccgcc gcagccggcc
     1921 gcgccgcccc ctccgtcggc tccggttggt agtcccgcgc cgccgccacc cgggccgccg
     1981 ctcgcggctg aggttcccgt ggcgaccaac ccggccgacg gtctgcacgg catgatggtt
     2041 cctcctggag tcggaccatg atgcggcgca agctgttcgg ggtggtggta atcgtcgcgg
     2101 cagtagcgac cggatgcggt tggcgggggc tgaattccgt gccgctgccc ggcgtcgaag
     2161 gcgtcggccc aggcgcgttc accgtgcagg cgcagatgcc cgacgtggac aacatcgagc
     2221 agaactcccg cgtacgcgtg ggtgatgtca acgtcggcac cgtgaccaag ctggagcggc
     2281 agggctggca tgcgctggtc accatgaagc tcaactccga cgtcgaactg cccgccaacg
     2341 caaccgccaa ggtgggtcag accagcctgc tgggctcaca gcacatcgaa ctggcgccgc
     2401 cggtcgacgc gccgccggaa gggcggttgc gtgacggctc cctgatcccg ctggcgtccg
     2461 ccggtgcgta tccgacgacg gagcaagcgc tggccgcaat cgccttgctg ctcaacggcg
     2521 gtggattcgg caacattcac gacatcaccc aggcactgag caccgccttc gccggccgag
     2581 agaacgacct gcgcagcctc atcgagcaac tcgacaaggc gatcggctat ctcgacgacc
     2641 aaaaaggcga catcatcgcc gcgtcggaaa gcctcaacaa tctgatgggc cagatcgccg
     2701 aacaaaagcc ggtggttgac aaggcattac gcaccatccc ggatgcgctg gccgtgctgc
     2761 gggatcagcg ccagaacc
//
LOCUS       DATADN010000815         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_703929, whole genome shotgun sequence.
ACCESSION   DATADN010000815 DATADN010000000
VERSION     DATADN010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703929"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1185)
                     /locus_tag="VHH53_10605"
                     /pseudo
     CDS             complement(147..1185)
                     /locus_tag="VHH53_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599356.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1563..>1936
                     /locus_tag="VHH53_10610"
     CDS             1563..>1936
                     /locus_tag="VHH53_10610"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2290617.1"
                     /translation="MRVFVAGATGAIGKQLVPRLVEAGHEVHGMTRSESKQAMLHELG
                     AVPVVADALDPDQVAQAVGRARPDVIVHQLTAIGGLDMRHLERGFALTNRLRTEGTDH
                     LLSAGQAVGVRRFVAQSFGPTS"
BASE COUNT          295 a          581 c          674 g          386 t
ORIGIN      
        1 gttcaaaacc tcggcctgct caaggtcgtg ggcgtgctgc tgatcttggc cggaggtgta
       61 ctggtggtgc gcttctagtg gtgtgtctca gttttggttg actggttgag ggcgactctt
      121 cctcggcgga ccttctcgag gatggactag gcggacgcag tccagatcag tggcttgggg
      181 ttggcgttgg tgacgcggag gtagtcctcg atgctggcga tcaggtcgag aaccctgggg
      241 aagctgccgc gtcggatgtt cttgtcggtg agttcgcgga accagcgctc gaccaggttg
      301 agccagctcg acgaggtggg tgtgaagtgc aggtggaacc gggggtgctg ggccagccat
      361 gccttggtct cggggtgctt gtgggtgtgg tagttgtcca ggatcagatg gacctgcagc
      421 gccttgggca cgttccggtc gatggtgcgc aagaagacca ggaactcttc gtgccggtgc
      481 ttgggtaggc attggccgag cacggtgccg gtggccacgt cgagcgcagc gaacagggtc
      541 gtggtgccgt tgcgcttgta gtcatgtgtc atcgtgccgg cccgcccctt ggtcatcggc
      601 agcgagggct gggtgcggtc gagtgcctgg atctggctct tctcgtccat gcacagcacc
      661 accgccttct tcggcgggtt caagtacagg ccgacctcgt cgatcagctt gtcctcgaac
      721 tgtgggtcgt tggacagctt gaaggtctcc acccgatgcg gcttaagccc gcgggcgtcc
      781 cagatccgtt gcaccgtcgc cgggctgacc ccgacccgat cggccatggt gcgacacgac
      841 cggtgggtgt gactatcggg tttggagtgc agggttaact cgacgatcga cgatctcggc
      901 gatctactcc tcgctgatca aggacttgcg gccgcgcccc cggcggacct gacccaactt
      961 ggccagcccg tcctcggcga accggtcccg ccacgcgcgg acggtgccga ccgagacccc
     1021 gatccgctgt gcgatgtggc tgttggccac cccgttggcc gcgagcagca gtgccttggc
     1081 tcgctgcacc tgccggtgcg gcgcggcctg tgaggccgct aacccctcca acgccacccg
     1141 ttgaccttcg tcgatgacca gcgccgcggc aggaggacga ctcaccaccc caatctacga
     1201 cccaaacact agattatttg cgagacacac accactagca gctcatagcc cgatgtgtag
     1261 gccagcaggt cgcccaagcg gttcttcacg accgccgccc ccggacagcg tgttctgggc
     1321 cgatcgaatg gagtctcgcc gcccctggac gacgacgcac ttgccttcgt gcccgatgcc
     1381 gatcgcgagc gatcttcacg atgcgctgtg ggcgacgttc gtcgtgcagg cgttgccagc
     1441 cggtccccag cattgccacc cccgcgcccg cgttcccgtg ccggggtgct cggaggtgtc
     1501 acagccgggg ctgccgtctt gtcttgtgga gtgcaggtaa tcctcgatga aggagtttga
     1561 tgatgagagt gtttgtcgca ggagcgacgg gggcgattgg aaagcagctg gtgccacgtc
     1621 tggttgaggc gggtcatgag gttcacggca tgacgcgtag cgagtcaaag caggcgatgc
     1681 tccacgagct gggcgctgtg ccggtggtcg cggacgcgct cgatcccgat caggtcgcac
     1741 aggccgtggg cagggcgagg ccggacgtga tcgtccatca gctgaccgca attggagggt
     1801 tggacatgcg tcacctcgaa cgcggtttcg cgctgaccaa ccgtctgcgg accgagggga
     1861 cggatcacct gctctcggcc gggcaggcgg tgggggtgcg gcggttcgtt gcgcagagct
     1921 ttggccccac gtcgta
//
LOCUS       DATADN010000816         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_843585, whole genome shotgun sequence.
ACCESSION   DATADN010000816 DATADN010000000
VERSION     DATADN010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843585"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VHH53_10615"
     CDS             <1..1129
                     /locus_tag="VHH53_10615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2290618.1"
                     /translation="CHVPMLGRAQVSLPSRGPATGNRLPTPEGPALVDGNFVRVTSML
                     ERAHTIAEEVLFPRALDVDAGGVIPGSHFDVLAEHGFYGLASQPESTSWNGIFEILAG
                     GCLTTTFTWIQHHGLVRALAGTANVELREKYLDAAVRGSLRGGVAYAGAIPRPPRLWA
                     TATAGGWLISGDAPFVTGWGIIDMLLVSARDIAGGPQGSIVSGLVGAEAGGGITVKEL
                     HLVAAAASNTVQLRFEGYFLPAEKVTAEIIHADFLARTHIALRRNGSLAMGVAGRCIR
                     MIGGVGRPELAELLAVEQGTIRDRLDRGLSEPATLSTARAAASEFAYRCAGALVAATG
                     SSGIFAGRHAQRLVREAAFTLVAAGRPDIKENLLSLFGSAR"
     gene            1213..>1613
                     /locus_tag="VHH53_10620"
     CDS             1213..>1613
                     /locus_tag="VHH53_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2290619.1"
                     /translation="MRRAHALPVGLALAAVASLTLACGGATGNTAQRAPAGVVPAGLE
                     RFYGQDLSWGSCAAFATSQRDQEAYSEPGLQCTHLEVPLDYAKPDGKVIKVGLLRRPA
                     SDPQQRIGSLVMNPGGPGGSGMSAAAGKAGD"
BASE COUNT          261 a          498 c          570 g          284 t
ORIGIN      
        1 gtgccacgtg ccgatgctag ggcgggcgca ggtgtcgctg ccgtcgaggg ggcctgccac
       61 aggcaaccgg ctgccgacgc ccgagggccc agcattggtc gacggtaact ttgtgcgcgt
      121 gaccagcatg ctggaacggg cccacacgat tgcggaggaa gtgctgtttc cccgggcttt
      181 agatgtcgac gctgggggag tcattcctgg tagccatttc gacgtgttgg ctgagcacgg
      241 tttttatggc ctggccagcc agccagaaag cacgtcgtgg aacggcattt tcgagatcct
      301 ggcaggaggc tgcctcacca cgacgttcac ctggatccag caccacgggc tggtgcgggc
      361 gctggctggc acagcgaacg tggaactacg cgaaaagtac cttgacgctg ccgtccgggg
      421 cagcctgcgc ggcggggtgg cgtacgccgg cgcgatcccg cggcctccgc ggctatgggc
      481 gacggccacc gccggtggtt ggctgatcag cggtgacgct cccttcgtga cggggtgggg
      541 aatcatcgac atgctgctgg tctcggcacg cgacatcgcc ggaggaccgc aaggctccat
      601 cgtcagcgga cttgtcggcg ccgaagccgg cggtggcatc accgtcaagg aattgcactt
      661 ggtcgccgct gcggccagca acactgtcca gctgcgcttc gagggctatt tccttccggc
      721 tgagaaggtc accgcagaga ttatccatgc cgacttcctc gccaggacgc acatagcctt
      781 gcggcgcaac ggcagcctcg cgatgggtgt cgctgggcgg tgcatccgga tgatcggcgg
      841 ggtggggaga cccgagttgg cggagctgct tgcggtggag cagggcacca tccgcgaccg
      901 cctcgacagg ggactgtccg aaccagcgac gctgtccaca gctcgtgctg ccgcgtccga
      961 gttcgcgtac cgttgcgccg gtgcacttgt ggcggcgacc ggcagttccg ggatctttgc
     1021 gggccggcac gctcagcggc tggttcggga ggccgcgttc acgctcgtcg ccgccggcag
     1081 gccagacatc aaagaaaacc tgctgagcct gttcgggagt gctcggtaaa gagtctgcaa
     1141 cgtatgcgtg actgcttgca catgacggct acactgggcg accacaccgg ggctgagggg
     1201 gaggatgctg ggatgagacg tgcgcatgca ttgcctgttg ggttggcgct tgcagcggtg
     1261 gcgtccctca cgctggcgtg cgggggtgcc accggcaaca ccgcacagcg tgcacctgcc
     1321 ggtgtagttc ccgctggctt ggaacggttc tatggccagg acttgtcctg gggcagctgc
     1381 gccgcattcg ccaccagcca acgtgaccag gaagcctatt ccgagcccgg cttgcagtgc
     1441 acgcacctcg aggtgccgct ggactacgcc aagcccgacg gcaaggtcat caaagtcggc
     1501 ctacttcggc gaccggcatc agacccccag cagcgcatcg gttctttggt gatgaacccg
     1561 ggcggtccgg gcgggtctgg gatgagcgcg gcagccggca aggccggtga cat
//
LOCUS       DATADN010000817         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1013686, whole genome shotgun sequence.
ACCESSION   DATADN010000817 DATADN010000000
VERSION     DATADN010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013686"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VHH53_10625"
     CDS             <1..293
                     /locus_tag="VHH53_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2290620.1"
                     /translation="RTGSLDSLLDADGAGTLLEEIVGTADAELGRVEYRTTLAPLLDR
                     LPERECTILKLRFFAGLTQSEIAQQIGLSQMHVSRLLSRTLARLRQQLRRES"
     gene            307..>1532
                     /locus_tag="VHH53_10630"
     CDS             307..>1532
                     /locus_tag="VHH53_10630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2290621.1"
                     /translation="MEHVVVVGAGLGGLRTVESLRRAGFPGQISLVGDEPHEPYDRPP
                     LSKQILAGQWPEERAALHHGDLADLDVSLHLGRPAIGVDGTAVKLADGERLRYDALVV
                     ATGVLPRRLPGQPDHPELHVLRTLEDCRGLRDSLSRARSLLVIGAGFIGAEVAATARI
                     AGLDVTMIEALPVPFARVLGEQMGRLCARLHTDHGVTVRCGVRLEDFPDADRNGGIAA
                     RLADGSILRADCGVVGVGTVVDGGWLAGLGVSTEGGLRCDSTGLVEGTTNVYAVGDVA
                     AWQHPTVGDRRRIEHWTSATEQAAVVAQRIAGAEITRQADVVPYFWSDQYGLTLQLIG
                     RCDLATSVEVLHDPGTIKGTVAGYFQAGNLAPPGRRAAPLPVPAASGVPTAAAAPSAL
                     QLGGQVAPGGGVRPADP"
BASE COUNT          239 a          531 c          532 g          230 t
ORIGIN      
        1 accgcaccgg ctcactggac agcctgctcg acgccgacgg cgccggtacg ttgctggaag
       61 agatcgtcgg tacggctgac gccgaactcg gccgagtgga ataccgcacc accttggccc
      121 cgctgctgga caggctgccc gaacgcgagt gcaccattct caaactgcgc tttttcgccg
      181 gcctgaccca aagcgagatc gcgcaacaga tcgggctttc gcagatgcac gtctcccgcc
      241 tgctgtctcg gacgctggcc cggctacggc agcagctacg cagggagtcc tgaaccggag
      301 gcgcgaatgg agcacgtagt ggtcgtcggc gctgggctcg gcggcctacg taccgtggag
      361 tccttgcgcc gcgcagggtt tcccggccag atcagcctcg tcggggacga gccgcatgag
      421 ccctacgacc ggcccccgct gtccaaacag atcctcgctg ggcagtggcc cgaggaacgg
      481 gccgccctgc accatggcga cctggcagac cttgatgtat cgctgcactt ggggaggccc
      541 gcgatcggcg tcgacggcac cgctgtgaaa ctcgccgatg gcgagcggct tcgctacgac
      601 gcgctggtcg tggcgaccgg tgtactgcca cggcggctgc ccggccagcc tgaccacccg
      661 gagctgcacg tgctgcgcac gctggaggac tgccgaggcc tgcgggactc cctctcccga
      721 gcccgttcac ttctggtgat cggggccggc ttcatcggtg ccgaagttgc cgccacggcc
      781 cgcatcgccg gcctcgacgt gaccatgatc gaggcgttgc cggtaccgtt cgcccgagtc
      841 cttggggaac agatgggtcg actgtgcgcc cgattgcaca ccgaccacgg ggtgacagtg
      901 cgctgcgggg tacggctgga agactttccc gacgccgacc gcaacggcgg catcgccgcc
      961 cggctggctg acggaagcat cctgcgcgcc gactgcggcg tggtgggtgt gggcaccgtc
     1021 gtcgacggcg ggtggctggc cgggctcggc gtatccaccg aaggcgggct gcggtgcgac
     1081 tccaccggcc tggtggaggg caccaccaac gtctacgcgg tcggcgacgt cgctgcctgg
     1141 cagcacccca cggtcgggga ccggcggcgc atcgagcatt ggacatcggc caccgagcag
     1201 gccgcggttg tcgcacaacg aatcgccgga gcggagatca ccaggcaggc cgacgtcgtg
     1261 ccgtacttct ggtctgacca gtacgggctg acgctgcagc tgatcgggcg ctgtgacctg
     1321 gcgacttccg tcgaggtgct gcacgacccc ggcacgatca agggcaccgt cgccggctac
     1381 ttccaggcgg gaaacctggc accaccagga aggcgagcag caccactccc ggtgccagca
     1441 gcatccggcg tacccaccgc ggcggcagcg ccatcagcgc tccagctggg tggccaggta
     1501 gcgccgggag gcggtgtacg cccggctgat cc
//
LOCUS       DATADN010000818         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1295072, whole genome shotgun sequence.
ACCESSION   DATADN010000818 DATADN010000000
VERSION     DATADN010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295072"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1567
                     /locus_tag="VHH53_10635"
     CDS             <1..>1567
                     /locus_tag="VHH53_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX2290622.1"
                     /translation="ATITLQNYFRLYAKLSGMTGTAQTEAAELHQIYKLGVVSIPTNR
                     PMIRVDQPDLIYKTEEAKFDAVAADIAQRHAKGQPVLVGTASVERSEYLSKALVAAGI
                     PHSVLNAKQHEKEAMIISEAGRKGAVTVATNMAGRGTDIMLGGNPEFLADTALRARGW
                     DPVETPEQYEAAWEAAVNEARGEVKAEAREVIATGGLYVLGTERHESRRIDNQLRGRS
                     GRQGDPGESRFYLSLRDELMVRFNGPMVESIMTRLRMEDDIPIEAKMVSRAIQSAQTQ
                     VEQQNFEIRKNVLKYDEVMDLQRKVIYAERRRVLEGEDLRDQVEHMMTDVITAYIDGA
                     TENGYAEDWDLDQLWTALRTLYPVGIKHEDLGDDAGDLTREVLLAAVLDDARAAYARR
                     EAALEQAAGAGAMRELERRVVLSVLDRKWREHLYEMDYLKEGIGLRAMAQRDPLVEYK
                     REGFEMFSALLEALKEEAVGFLFNVQVQVAEPVAADPAQAPAPVAPTTPAPASPAPVA
                     APALPQLDEQPEPA"
BASE COUNT          288 a          497 c          552 g          230 t
ORIGIN      
        1 gccacgatca ccctgcagaa ctacttccgg ctctacgcga agctctcggg catgacgggt
       61 accgcccaga ccgaggcggc cgagctgcac cagatctaca agctgggcgt ggtgtcgatc
      121 cccaccaacc ggccgatgat ccgcgtggac cagccggacc tgatctacaa gaccgaagag
      181 gcgaagttcg acgcggtcgc cgccgacatc gcgcagcggc atgctaaggg gcagccggtg
      241 ctggttggca ccgcgagcgt ggagcgttcc gagtacctgt ccaaggcgct ggtggccgct
      301 ggcatcccgc attcggtgct caacgccaag cagcatgaga aggaagcgat gatcatctcc
      361 gaggccggcc gtaagggtgc ggtcacggtg gccacgaaca tggctgggcg cgggaccgac
      421 atcatgctgg gcggcaatcc ggagttcctc gccgacaccg cactgcgggc ccgcgggtgg
      481 gatccggtgg agactcccga gcagtacgag gccgcctggg aagccgccgt gaacgaggcg
      541 cgcggggagg tcaaggctga ggccagggag gtcatcgcga ccggcgggct gtacgtgctg
      601 ggcaccgagc ggcacgaatc gcggcggatc gacaaccagt tgcgtggccg ttctgggcgc
      661 caaggtgacc ccggcgagtc ccggttctac ctctccttgc gcgacgagct catggtgcgg
      721 ttcaacggtc cgatggtgga gtcgatcatg acccggctgc ggatggagga cgacatcccg
      781 atcgaggcca agatggtcag cagggcgatc cagtccgcgc agacccaggt cgagcaacag
      841 aacttcgaga tccgcaagaa cgtcctcaag tacgacgaag tcatggatct tcaacgcaag
      901 gtgatctacg ccgagcgccg ccgggtactc gaaggcgagg atttgcgcga ccaggtcgag
      961 cacatgatga cggatgtgat caccgcctac atcgacggtg ccaccgaaaa cgggtatgcc
     1021 gaggactggg atctcgatca gctgtggacg gcgctgagga cgctgtaccc ggtgggtatc
     1081 aagcacgagg acctcggtga cgacgctggt gacctgaccc gtgaggtgct gctggccgcc
     1141 gtgctcgacg acgcgcgcgc tgcctacgcc cgccgcgagg cagcactcga gcaggcggca
     1201 ggtgcgggtg cgatgcggga gctcgagcgc cgcgtggtgc tctcggtact ggaccgcaag
     1261 tggcgcgagc acctctacga gatggactac ctcaaggagg gcatcgggct gcgggcgatg
     1321 gcgcagcgcg acccgctggt ggagtacaag cgcgagggtt tcgagatgtt cagcgcgctg
     1381 ctcgaggcgt tgaaggagga ggccgtcggg ttcctgttca acgtgcaggt ccaggtcgcc
     1441 gagccggtcg ctgccgaccc cgcccaagcc cccgctccgg ttgccccgac cacccctgca
     1501 ccggccagcc cagcgccggt cgctgctccg gcgcttccac agctcgacga gcagcccgag
     1561 ccggcat
//
LOCUS       DATADN010000819         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1464019, whole genome shotgun sequence.
ACCESSION   DATADN010000819 DATADN010000000
VERSION     DATADN010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464019"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1118
                     /locus_tag="VHH53_10640"
                     /pseudo
     CDS             1..1118
                     /locus_tag="VHH53_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611777.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1288..>1537
                     /locus_tag="VHH53_10645"
     CDS             1288..>1537
                     /locus_tag="VHH53_10645"
                     /inference="COORDINATES: protein motif:HMM:NF037501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HEX2290623.1"
                     /translation="MAGKPPKHHSATDLQAQLKMLTRRLGSMGFPFDVNKQTAGTLGR
                     ELTIVRNARAHGDPLTRLDAWRAHDFCVRLLEYFGDAEG"
BASE COUNT          289 a          555 c          471 g          222 t
ORIGIN      
        1 gaggcaggtg aggtggtgcc gtacatggac tggaaggacc gccggatagc ccagctcgag
       61 cgtgagctgg ccgagtcgca gcagaccgtc gagatcttga aggcggccac aactttcttc
      121 gcgcgggagt gcgacccgcg cccaccactg gagagctctg cgagttcatc gaggtccacc
      181 gtgaacggtt cggagtcgca ccgatctgcc gagcgctgag cagcgctggc ctggcgatcg
      241 ctccgaacac ctactacacc cggcgtcggc ggggaatctc gaagcgggcg ctgtgggaca
      301 ccaccctcac cgaggtcctg gctggctact acgagtccga cgagcacggg cggctgcggc
      361 cggagtcgct gtacggcagc ttgaagatgt gggcccacct gaaccgggag ggcatcgagg
      421 tcgcccggtg caccgtggaa cgcctgatgc ggctcaacgg ctgggtcgga gtgacccgtc
      481 gggcgcagcc gcggaccacg atcagcgacc ccgacgccct cgagcgtgcc cccgacctcg
      541 tcgaccggca gttcgccacc cacgcacccg atctgctgtg ggtcgctgac ttcacctacg
      601 tgctgcggat cgggggacgg tggtgctaca ccgcgttcac catcgacacc ttcgctgacg
      661 cgatcgtggg ctggcaggtg acccagacgc ccaccgcggc catggtcgag gccagcttcg
      721 cccacgccat cgagcaccgc caccgccagg gccacccggt ggccgcgggc gcgatccatc
      781 actccgacgc cgggacccag tacaccgcga tcaggttcgc cggcagcctc gccgaggcag
      841 acttccgtcc ctcgatcggc agcatcggcg acgcctagga caacgccctg gccgagacca
      901 tcatcgggct ctacaagtgc gagtgcgccc gggtgggctc acccttcaac cccgacgggt
      961 tcaccaccgt cagcgacgtc gagacggcca ccgccgagtg ggtgcactgg ttcaacaacc
     1021 accgcctgct ccaccggctc ggacgccgac caccagccga agcggaagcc gagcactacg
     1081 ctcaacaaca agccgaccaa cccctcggca ccaactgaac ccggagtgga caaaacccgg
     1141 gacggttcac agtgaccttc gtgcaagaac tctccgtaac ccgggactcg accacctctc
     1201 ttcgcgactg aacccgatca ttgcgatcgc atcaccgttg atcttggcgg gcatccatgg
     1261 acagtcgtcc tcaaccaact ggacgagatt gccggaaagc cacccaagca ccacagcgcc
     1321 acggacctcc aggctcagct caagatgctc acacggcgcc tgggcagcat gggcttccca
     1381 ttcgacgtca ataagcagac ggcaggcacc ctcgggcgtg agctcaccat cgtccgcaac
     1441 gctcgcgctc acggcgatcc gctcacccga ctcgacgcat ggcgggccca cgacttctgc
     1501 gttcgactcc tggagtactt cggcgatgcc gagggcc
//
LOCUS       DATADN010000820         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1464111, whole genome shotgun sequence.
ACCESSION   DATADN010000820 DATADN010000000
VERSION     DATADN010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464111"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..729
                     /locus_tag="VHH53_10650"
     CDS             178..729
                     /locus_tag="VHH53_10650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX2290624.1"
                     /translation="MRAREPAQLAELTAEQLRVRLADALRIYVTAMRYPPGTARHRSP
                     MWLEHMLRAGWRCMAAFESEALVGIAYGYRGAPGQWWHDEVHRGLMQIDPVAAQRNLT
                     DYFELTELHVHPRAQGRGIGEALLTELLSRVDAPRALLSTPEGPTRAWRLYRRIGFTD
                     LLRNYHFTGDSRAFAVLGRTLPL"
     gene            923..>1684
                     /locus_tag="VHH53_10655"
     CDS             923..>1684
                     /locus_tag="VHH53_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2290625.1"
                     /translation="MAQVNATRGAGLLYPEVPVTDQQAHPLDENLRAEVDRELAAFLQ
                     AKRDDAAQIDSHFVAAVDSLSDFVLGGGKRLRPTFAWWGWRGAGGSPTGPETHAVLRA
                     VSSLELIQACALVHDDLMDDSTTRRGAPTVHVDFAARHRAAGWLGTPERFGAATAILL
                     GDIALAWADDMLRVADLWPDQMGRALGVWQAMRTEVLAGQYLDVLTQAGGDEAPETAL
                     QINRYKTAAYTVERPLHLGAAIAGACPALVDAYGRF"
BASE COUNT          290 a          554 c          560 g          280 t
ORIGIN      
        1 tcgaacattt gttcgacacg acttaagagt aaccccccta catgcggtcc cgtcaagtcc
       61 ggctcgtagt aagggggcag gccgagcctt ggcgtggtct gataaggccg tgcagcgcgc
      121 cggggccatc cgcttagcag ccgcgccggc cgatactgga ggtcggcggt gacagcaatg
      181 cgcgcccgcg aaccggccca gctggccgag ctgacagccg agcagctgcg cgtgagactc
      241 gctgacgcgc tgcggatcta cgtcactgca atgcgctacc cacctggcac ggcacggcat
      301 cgaagcccga tgtggctgga gcacatgctg cgcgccggct ggcggtgcat ggcggccttc
      361 gaaagcgagg cgctggtggg tatcgcctac ggctaccggg gagcgcccgg ccagtggtgg
      421 catgacgagg tgcaccgtgg actcatgcag atcgaccccg tcgcggcgca acgcaacctc
      481 accgactact ttgagctcac cgaacttcat gtgcatccac gagcacaggg ccgcggcatc
      541 ggcgaagcgc tgctcaccga actgctgtcc agggtggatg ctccgcgcgc gttgctgtcc
      601 actcccgagg gccccacaag ggcgtggcgg ctataccgtc ggatcggatt caccgacctc
      661 ctgcgcaact accacttcac cggggattcc cgcgccttcg ccgtactggg ccgcacgctg
      721 ccgctctaac ccgcaagcca aggttggcag ccgttaacgg cggtagcgac tggcgtggag
      781 tacttgaaga gcgccgactg aaccggaaca gcactggcgc cgaacccggg cacatgttga
      841 caagtagttc ctttatcgtc ggcatgcacg gcgggtgccg ggtggcctac gtagtgctgt
      901 caccacgatc gcaggaggtt tcatggcgca ggtaaacgcc acccgcggag ccgggcttct
      961 gtacccagag gttccggtga cagatcagca ggcgcacccg cttgatgaga acctccgtgc
     1021 cgaggtagac cgtgagctcg ccgccttcct ccaagccaaa cgcgatgacg cggcacaaat
     1081 cgattcgcat ttcgtcgcgg cggtggactc actgagcgac ttcgtactcg gcggcggcaa
     1141 gcgactgcgc ccgacgttcg cctggtgggg ctggcggggc gcaggcggat ccccgactgg
     1201 cccagagacg catgcggtcc tccgcgcggt gagctccctg gaactcatcc aggcctgtgc
     1261 cctggtgcat gacgatctga tggatgactc cactacccgg cgaggcgcac cgacggtgca
     1321 cgtcgacttc gccgcccggc atcgggctgc cgggtggttg ggcacgccgg aacggtttgg
     1381 cgccgccacc gccatcctgc tcggcgacat cgccctggca tgggccgatg acatgttgcg
     1441 tgtggcagat ttatggcctg atcagatggg cagagcactt ggcgtgtggc aggcgatgcg
     1501 caccgaggtg ctcgccgggc agtacctcga cgtgctgact caggccggcg gcgacgaggc
     1561 gcccgagacc gcgctacaga tcaaccggta taagacggcc gcctataccg tcgagcgtcc
     1621 tctgcaccta ggggcggcaa tcgccggagc gtgccccgcg ttagtggacg cctacggccg
     1681 tttt
//
LOCUS       DATADN010000821         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_557801, whole genome shotgun sequence.
ACCESSION   DATADN010000821 DATADN010000000
VERSION     DATADN010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557801"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VHH53_10660"
     CDS             <1..241
                     /locus_tag="VHH53_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290626.1"
                     /translation="RVDSELAQERARLLEAQRIARIGSFTWEWGTSNKLSYSPALAEL
                     FAGRRAGSSGRTRLAAGSRQGGPANPVAGVTRTGL"
     gene            213..>2013
                     /locus_tag="VHH53_10665"
     CDS             213..>2013
                     /locus_tag="VHH53_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2290627.1"
                     /translation="MPESPALVCDGGMVVQANLAVTRLAGRWLPDSLIGLPLHRLLTG
                     AGTDAELLKPEGHSIPVRVTRWIVPGTNLLVVFLVDVSDLRLAAEAPAEPVVNLRPWQ
                     AGATRCSRGRSDLFSEPRDLFSEPRVDSELAQERARLLEAQRIARIGSFTWEWGTSNK
                     LSYSPALAELFAGRCVDPVDPLRHVHPEDLPTLLRRVEEMVSDPGVGTLEAEFRGLPE
                     YGEAFYVCRLRAQRSDSGEILRLMGTVQDVTSERALERELREEQRRLAEAQRVARIGT
                     WEWEPGSDRLVWSDMMQDLYGAAPASDQPTYQAYLSLVHPDDREWVDELWRQLLADHV
                     PVECEHRVLRPDGTVRTFRCHGAVIADTSGTLRVVGTAQDVTDQRAAEIRMMRSSQRF
                     ADLVAVAPVGIGIFDDADRLIDANEALCKLLGYDLETLRGMSSEALTHPDDRAERLRT
                     MRNHRAAPCESYTVPQRMLLRADGEPVYCELHISTSVQDDGQRFMLVVFTDVTERRRV
                     AEALKYRATHDELTGLPNRAAIKEILTELLALPDRDLAVLFCDIDNFKRVNDALGHDA
                     GDEMLVALARWLRDGLPEGCTGGRLSGDVFVVIC"
BASE COUNT          312 a          627 c          722 g          352 t
ORIGIN      
        1 ccgggtggac agtgagcttg cgcaggagcg cgcgcggctg ctggaggcac agcggattgc
       61 ccggatcggc agtttcacgt gggagtgggg taccagcaac aagctcagct acagccccgc
      121 tctggcggag ctgttcgctg gacgccgggc cggtagctcg ggccgcaccc ggctggccgc
      181 tggcagccgt cagggtggac cagccaatcc cgttgccgga gtcacccgca ctggtctgtg
      241 acggcggcat ggtcgtccag gccaatctgg cagtgacccg gctcgccggc cgctggcttc
      301 cggactcgct gatcgggctg ccgctgcacc gtctcctcac gggtgccggc actgatgccg
      361 aactcctcaa accggaaggg cactcaatac cagtgcgggt gacgcgctgg atcgtgccgg
      421 gcacgaattt gcttgtggtg ttcctggtcg acgtctccga tctacgcttg gccgccgagg
      481 caccggccga gccggtcgtc aatctcaggc cttggcaggc cggtgcgacc cggtgctcgc
      541 gagggcgctc cgatctcttc agcgaacccc gggacctctt cagcgaaccc cgggtggaca
      601 gtgagcttgc gcaggagcgc gcgcggctgc tggaggcaca gcggattgcc cggatcggca
      661 gtttcacgtg ggagtggggt accagcaaca agctcagcta cagccccgct ctggccgagc
      721 tgttcgctgg acgctgcgtc gatcctgtcg accccttacg gcacgttcac cccgaagacc
      781 tgccgacctt gttgcggcgg gtggaggaga tggtctccga ccctggtgtg gggaccctgg
      841 aggcggaatt tcgtgggttg cccgaatacg gcgaggcctt ttacgtgtgc cggctgcgcg
      901 cgcagcggtc ggacagcggg gagatcctgc gcctgatggg caccgtgcaa gacgtcacgt
      961 cggaacgggc gctggagcgc gagctgcgtg aggagcagcg ccggttggcg gaggcgcagc
     1021 gggtggcccg aatcggtacc tgggagtggg agccgggttc ggaccggctc gtgtggtcgg
     1081 acatgatgca agacctctat ggggcggcgc cggcctcaga ccagcccacc taccaggcct
     1141 atctctcgtt ggtacacccc gatgaccggg aatgggtgga cgagctgtgg cggcagctgc
     1201 ttgccgacca tgtgccggtg gagtgcgagc atcgggtgct gcggccggac gggacggtac
     1261 gcacgttccg gtgtcacggc gccgtcatcg ccgacacatc cgggaccctg cgtgtggtcg
     1321 ggaccgctca ggacgtcact gaccagcgag ccgccgaaat ccggatgatg cgttccagcc
     1381 agcgatttgc tgacctggtg gcggtcgccc cggtaggcat cgggatcttc gatgatgccg
     1441 atcggctgat cgacgccaac gaggcgctat gcaagctgct gggttatgac ctggagacac
     1501 ttcgggggat gagttccgaa gcgctcacgc acccggacga ccgagctgag cggctgcgca
     1561 ccatgcgtaa ccaccgtgcg gcgccctgtg agagctacac ggtgccgcag cggatgctgc
     1621 tgcgggccga tggtgagccg gtgtactgcg agctgcacat ctcgacgtcg gtgcaagacg
     1681 acgggcaacg gttcatgctg gtcgttttca ctgatgtcac cgagcgccgc cgagtggccg
     1741 aggccttgaa gtaccgggcg actcacgacg agctcaccgg cctgcccaac cgggctgcga
     1801 tcaaggagat cctgaccgag ctgctcgcct tgcccgaccg cgacctggcc gtgttgttct
     1861 gcgatatcga caacttcaag cgggtcaacg atgcgttagg tcatgacgcc ggcgacgaga
     1921 tgctggtcgc gttggcgcgg tggctgcgcg acgggttgcc cgagggttgc accggcggca
     1981 ggctctccgg cgacgttttc gtggtgatct gct
//
LOCUS       DATADN010000822         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_411554, whole genome shotgun sequence.
ACCESSION   DATADN010000822 DATADN010000000
VERSION     DATADN010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411554"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..>1550
                     /locus_tag="VHH53_10670"
     CDS             334..>1550
                     /locus_tag="VHH53_10670"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX2290628.1"
                     /translation="MSATLVIALGLATPVPVAGGEPSVYLIPGLDRSAQLIQDKWGVP
                     HIYAQSTEDVFLVQGFNAARERLFQIDLWRRRGLGLLSEVFGPSYVDQDAATRLFLYR
                     GDMTAEWESYGPEAAMAATRFTAGVNAYVDWLARNPGLLPEEFRRLGYIPARWRPEDV
                     VRIRSHGLTRNLSSEVARSRVACVAGPEADQVRVRLEPDWHTSVPAGFDPCVVPEDVL
                     HTFELATQNVEFTGTDLRVVPIMSQEAVEGSNNWTIAPSRTATGRPILANDPHRAYSA
                     PSLRYIAHLSAPGLDVIGAGEPAQPGISLGHNDTVAFGLTTFRMDQEDLYVYQLDPHD
                     HGRYRFGDGWERMIVRHEQIPVAGEKPRNVELPFTRHGPVIKVDGQRNLAYAVRTTWL
                     QPGMSPYYGSLRLL"
BASE COUNT          259 a          498 c          521 g          272 t
ORIGIN      
        1 cgctgaaccg gcgtccgatg gcgattgtct cgcggagcag ctgttcgggc actgcgcatc
       61 cggttccggg cctgagctgc cgattctggc gcgagcggtg gtgcgccgca ctgccgccgg
      121 ccagaagtaa ccacagttca acacaagcgg gtagcgtcgc gaaccggaag ttcgtgcagc
      181 cagtccgacc ggtgttgatg ttcacccgat accaggtact cgaaaccggg ccgtggctgc
      241 catgaaccgg tccctgcgtg gccatctcgg gggagaacga gaatgtctaa acgcctcttt
      301 cgccggcgca ggccaggcag tgtcgtcgcg ttgatgagcg ccacgctggt gattgcgcta
      361 gggctggcca caccggttcc cgtagctggt ggcgaacctt cagtgtacct tatccccggc
      421 ctggaccggt ccgcgcagct gatccaggac aagtgggggg tgccgcacat ctatgcgcag
      481 agcacggagg acgtcttcct cgtccagggc ttcaacgccg cgcgggaacg gctgttccag
      541 atcgatttgt ggcgccgccg gggtctcggc ctgctgtccg aggttttcgg gccgtcttat
      601 gtggatcagg acgcggccac caggctgttc ctctaccgcg gcgacatgac ggcggaatgg
      661 gagagctacg ggcctgaggc cgcaatggcg gccactcggt tcacggcggg cgtcaacgcc
      721 tatgtggact ggctcgcacg gaatcctggg ttgctgccgg aggagttccg gcggctcggg
      781 tatatccccg ctcgctggcg gccggaggat gtcgtgcgaa tccggagcca tggcctgacc
      841 agaaacctct ccagcgaggt ggcccggtct cgggtcgcgt gcgtggctgg cccggaagcc
      901 gaccaggtgc gggtcaggct cgagccggac tggcacacgt ccgtgcccgc cgggttcgac
      961 ccctgcgtgg ttccggaaga tgtcctgcac accttcgaat tagccacgca gaatgtcgag
     1021 ttcaccggca cggacctgcg tgtggtgcca atcatgtccc aggaggcggt agagggcagc
     1081 aataactgga ccatcgcgcc gagcaggacg gcaaccggcc ggcccatcct ggccaacgat
     1141 ccgcaccgtg cctattcggc accgtcgctg cggtacatcg cgcatctttc cgcccccggc
     1201 ctcgacgtga tcggcgcggg ggagcccgct cagccgggca tttcgctcgg ccacaatgac
     1261 accgtcgcct tcgggctcac aaccttccgg atggatcaag aagacctcta cgtgtaccag
     1321 ctcgatccgc acgaccatgg caggtaccgc tttggcgacg gctgggagcg gatgatcgtg
     1381 cggcacgagc agatacccgt ggccggtgag aagccgagaa acgtcgagct gcccttcacc
     1441 cgtcacggac cggtgatcaa agtggatggg cagcgcaacc tggcctacgc ggtgcgcacc
     1501 acgtggctcc agcccggaat gtcgccgtat tacggcagcc tgcggttgct
//
LOCUS       DATADN010000823         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_704840, whole genome shotgun sequence.
ACCESSION   DATADN010000823 DATADN010000000
VERSION     DATADN010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704840"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VHH53_10675"
     CDS             complement(<1..138)
                     /locus_tag="VHH53_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX2290629.1"
                     /translation="MREVLPVVGSVLIPAAELQWRFSRASGPGGQGVNTTDSRVELSF
                     DV"
     gene            complement(218..>2150)
                     /gene="acs"
                     /locus_tag="VHH53_10680"
     CDS             complement(218..>2150)
                     /gene="acs"
                     /locus_tag="VHH53_10680"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX2290630.1"
                     /translation="RAFPPSEEFAAQANATAALYDQADADREGFWAQQARRLHWHTDF
                     EQVLDWSDAPHAKWFVGGKLNVAYNCVDRHVADGHGDQVAFHWEGEPGDTRTITYAGL
                     QREVCKTANALHSLGMQTGDRVAIQLPMIPEAVFAMLACARLGMPHSVVFGGFSAESL
                     RQRINDAQAKLVITADGQYRRGKPAPLKANTDEALKDASTVRQVVVVKRTDTDIDWTD
                     GRDLWWHELVDQQADQHTCEPFDSEHPLYILYTSGTTGKPKGILHTSGGYLTQCAYTH
                     DVVFDHKAGQDVYWCTADIGWVTGHSYVVYGPLANRATSIMYEGTPNTPHEGRHWELV
                     QKYGCTLYYTAPTLIRTFMKWGKEIPEKYDLSSLRVLGSVGEPINPEAWMWYREHIGS
                     SRCPIVDTWWQTETGAIMISPLPGVTVTKPGSAMRALPGISATVVDDSGNPVGHGEGG
                     YLVLDKPWPAMLRTIWGDDERYRETYWSRFADQGYYFAGDGAKYDDDGAIWLLGRVDD
                     VMNISGHRISTTEVESALVSHPTVAEAAVVGASDPITGQGIVAFVILRGDVAQDEAQG
                     EEAIKALRNHVAKEIGAIAKPRQIMVVADLPKTRSGKIMRRLLRDVAENREIGDVTTL
                     ADSSVMELISTGMQSGSQE"
BASE COUNT          336 a          711 c          712 g          391 t
ORIGIN      
        1 cacgtcgaag ctcaattcga cccgtgagtc ggtggtgttg acgccctgac cgccaggccc
       61 gctcgcgcga gaaaagcgcc actgcagctc ggccgccggg atcaagaccg agccgaccac
      121 cggcagcacc tcgcgcacct acctagcatg cccgttggag ggtgggccgt ctttgagacg
      181 tcctaccggg ggttggccat ccttgaagag ctaccaacta ctcctgggag ccggactgca
      241 tgccggtgga gatcagctcc atgacggacg agtcggccag cgtggtgacg tcaccgatct
      301 cacggttctc cgcgacatcc cgcaacagcc gtcgcatgat cttgccagat cgggtcttgg
      361 gcagatccgc cacgaccatg atctgccgtg gcttggcgat cgcgccgatc tccttggcca
      421 cgtggttgcg tagcgccttg atcgcctctt caccctgtgc ctcgtcctgg gcgacgtcgc
      481 cccgcagaat gacgaacgcg acgatgcctt gcccggtgat cggatcagag gcgcccacca
      541 ccgcggcctc agccaccgtt gggtgggaca ccaacgcgga ctccacctcg gtggtggaga
      601 tccgatgccc ggagatattc atcacgtcgt ccacccggcc aagtagccag atggcgccgt
      661 cgtcgtcgta tttggcgccg tccccggcga agtagtaccc ctggtcggcg aaacgcgacc
      721 agtaggtctc ccggtagcgc tcgtcatcgc cccagatggt gcgcagcatc gccggccacg
      781 gtttgtccaa taccagatag ccgccctcgc cgtggccgac cggattgccg gaatcgtcga
      841 ccacggtggc gctgataccg ggcagcgcgc gcattgccga acccggcttg gtcacggtga
      901 cgccgggcag cggcgagatc atgatcgcgc cggtctcggt ctgccaccag gtatccacga
      961 tcgggcagcg gcttgagccg atgtgctccc ggtaccacat ccaggcctcc gggttgatcg
     1021 gctcgccgac gctgcccagt acccgcagcg acgacaggtc gtacttctcg gggatctcct
     1081 tgccccactt catgaaggtg cggatcagcg tcggcgcggt gtagtacagc gtgcagccgt
     1141 acttctgcac gagctcccag tgcctgccct cgtgcggggt gttcggggtg ccctcataca
     1201 tgatcgacgt cgcccggttg gccagcggcc cgtagacgac gtagctgtgc ccggtgaccc
     1261 aaccgatgtc ggcggtgcac cagtagacgt cctgaccggc cttatggtcg aagaccacat
     1321 cgtgggtgta ggcgcactgg gtgaggtaac caccgctggt gtgcaggatg cccttgggtt
     1381 ttccggtggt gcctgaggtg tagaggatgt acagcgggtg ctcgctgtcg aacggctcgc
     1441 acgtgtgctg gtcggcctgc tgatcaacca gctcgtgcca ccacaggtca cgaccgtcgg
     1501 tccagtcgat gtcggtgtcg gtgcgcttga ccacgaccac ctggcgcacc gtggaggcat
     1561 ccttgagtgc ctcgtcggtg ttggccttca acggggctgg cttgccgcgc cggtactggc
     1621 catcagcggt gatcaccagt ttggcttgcg cgtcgttgat ccgctggcgc agtgactcag
     1681 ccgagaaacc accgaacacc acgctgtgcg gcatgcccag ccgggcgcag gccagcatgg
     1741 cgaagaccgc ctcggggatc atcgggagct ggatggccac ccggtccccg gtctgcatgc
     1801 ctaacgagtg cagcgcgttg gccgtcttgc acacctcacg ttgcaggccg gcgtaggtga
     1861 tggtgcgggt gtctcccggc tcgccctccc agtggaaggc gacctggtcg ccgtggccat
     1921 cggcgacgtg ccggtcgacg cagttgtagg cgacgttgag cttgccgccg acgaaccact
     1981 tcgcgtgcgg ggcgtcggac cagtcgagga cctgctcgaa gtcggtgtgc cagtgcagcc
     2041 ggcgggcctg ctgagcccag aagccctcgc ggtcagcgtc cgcttggtca tagagcgcgg
     2101 cagtggcgtt cgcctgggcg gcgaactcct cgctcggggg gaacgcgcgg
//
LOCUS       DATADN010000824         4336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_844692, whole genome shotgun sequence.
ACCESSION   DATADN010000824 DATADN010000000
VERSION     DATADN010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844692"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..710)
                     /locus_tag="VHH53_10685"
     CDS             complement(33..710)
                     /locus_tag="VHH53_10685"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2290631.1"
                     /translation="MPIAVDEIVETGWAGALSPVAEQIATAGTFLRTEIAAGRRYLPA
                     GPNILRAFTQPFDQVRVLIVGQDPYPTPGHAVGLSFSVEPNVRPLPGSLVNIFREYSD
                     DLGLPAPSNGDLSPWAQRGVLLLNRVLTVEPGRPGSHRGKGWEQVTEQAIHALVARPA
                     EPLVAILWGRDARTLRPMLSDVPCIESAHPSPMSADRGFFGSRPFSRANGLLADLGAD
                     PIDWALP"
     gene            complement(771..1760)
                     /locus_tag="VHH53_10690"
     CDS             complement(771..1760)
                     /locus_tag="VHH53_10690"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX2290632.1"
                     /translation="MGPAGDTASNSPPPEVGSVAGLGEFGLIARITSARDLPPATLLG
                     PGDDAAVLSAADGRVVVSTDVLVEGVHFRLDWSTPHQVGRKAVAANLADIAAMGAVPT
                     GLVVGLAVPGRTPVSTMDDLAGGMWEEAMQVGVGIVGGDVVNAAELVVAVTALGDLQG
                     RPPVTRAGAQVGDVLGLCGRLGWSAAGLAVLRRGFRSPLGVVGAHRVPEPPYGAGPQA
                     AAAGATAMIDVSDGLLADAGHLADASGVNIDIDSAALAVPSRLIEVASALGVDARHWV
                     LTGGEDHALLASFPAGVALPAGWTAIGAVRAGRCVTVNGERYEALPAGWKHFS"
     gene            1831..2067
                     /locus_tag="VHH53_10695"
     CDS             1831..2067
                     /locus_tag="VHH53_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2290633.1"
                     /translation="MVQAYILIQTEVGKAAAVAAAVASLPGVASAEDVTGPYDVIVRA
                     EAVDVDQLGQLVVARVQNVDGITRTLTCPVVHLS"
     gene            2079..2636
                     /locus_tag="VHH53_10700"
     CDS             2079..2636
                     /locus_tag="VHH53_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 domain-containing protein"
                     /protein_id="HEX2290634.1"
                     /translation="MAQSTVTPAANLPRWLLGIAVTLPVALVVVVLVVAAQVRGQPQG
                     PVGLVSVPAPAADSADCTRLLAALPDELDGGEQGTLQQRQLAATTPSGTAPAGAAAWG
                     EPPVVLRCGLDRPAALTATSRLLMISGVQFLKLPTTGTSTWVAVDRPVYIVVDLPPVT
                     GSAPLQQVATVIADILPQRDVNIPR"
     gene            complement(2640..3275)
                     /locus_tag="VHH53_10705"
     CDS             complement(2640..3275)
                     /locus_tag="VHH53_10705"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEX2290635.1"
                     /translation="MGLGGWRGGVPIFPSFESNDNGVPAGEHDATLIEDGLGDIAGDR
                     DRAGYRARHAQPDDDDDDDVPDEPTEPTEVSGDPVEPPTGAAVVETADEWEGPEDFRY
                     IVRPYTWTQGRTRPVQELAVETLVSTSDIGRDVATARSAEHSAIAGLCVDVRSVAEVA
                     ALLAVPLGVARVLLADMIDTGLVYVHRNPTEAGSPPGLSLMERVLAGLHQL"
     gene            complement(3417..>4336)
                     /locus_tag="VHH53_10710"
     CDS             complement(3417..>4336)
                     /locus_tag="VHH53_10710"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX2290636.1"
                     /translation="TIRDRALPAVDGSAVALALPGDPTRRELVRIAGARAGEVLSAVD
                     VVFPVLHGAFGEDGTIQGLLELADLPYVGSGVLASAAAMDKQFARKLLGIAGLPVTDA
                     VVLRSDHATLTPEQRQRLGLPVFVKPARAGSSTGITKVLDWSDLDSAVATARAVDPKV
                     LVEAAVTGREIECGVLEFPDGRIEASLPAEIRVVAADSAGWYDFDAKYLDDACEFDIP
                     AKLDDDVTTALRAMAVCAFRSLDCQGLARVDFFVGPDGALTVNEINTMPGFTPISMYP
                     RMWAVTGVDYPTLLSTLIDTAMARGTGLR"
BASE COUNT          738 a         1543 c         1409 g          646 t
ORIGIN      
        1 ctcccggctg ctggcggcgt cagcaggcga ggtcagggca gggcccagtc gatcgggtcg
       61 gctcccaagt cagcgagcaa cccgttggcg cggctgaagg ggcgggagcc gaagaacccg
      121 cggtcagccg acatcggtga cggatgcgcg gactcgatgc acggcacgtc ggagagcatc
      181 ggacgcaggg tgcgcgcgtc ccgaccccac aggatcgcca ccagcggctc cgccggccgc
      241 gccaccaagg catggattgc ctgttcggtg acctgctccc accctttacc acggtgcgac
      301 cccggccgtc cgggctcgac cgtgagcacc ctgttgagca gcaacacgcc ccgctgcgcc
      361 catgggctga ggtctccgtt ggacggggcg ggcaggccga ggtcgtcgct gtattcccgg
      421 aagatgttaa ccaggctgcc cggcagcggc cgcacattcg gctcgaccga aaacgacagc
      481 cccaccgcgt gccccggggt cgggtaaggg tcctggccga cgatgagtac ccgtacctgg
      541 tcaaacggct gggtgaatgc ccgcaggatg ttgggtccgg ctggcaggta gcgtcgccct
      601 gcggcgatct cggttcggag gaacgtcccg gcagtggcga tctgctcggc taccgggctg
      661 agggcaccgg cccagccggt ttccacgatc tcatcaacag ctatgggcac ggccgggcag
      721 cctagcggct ggtcgtgagt ggcattgagt gcgggaacac tgtttcccac tcacgagaag
      781 tgcttccagc ccgctgggag cgcttcatag cgctcgccat tgacggtgac gcaacggccc
      841 gcccgcacgg cgccgatcgc tgtccagccg gcgggcagcg ccactccggc ggggaagctc
      901 gccagtagcg cgtggtcttc tcccccggtg agcacccagt gccgcgcatc gacgcccagc
      961 gctgaggcca cttcaatcag ccgggaaggc accgccagcg ccgctgagtc gatgtcgatg
     1021 ttgacgcccg acgcgtcggc gaggtgccca gcatcggcga gcaagccatc cgagacatcg
     1081 atcatcgccg tggcacccgc cgcggcggcc tgcggccccg cgccgtaggg cggctcgggg
     1141 acacgatgcg cgcccaccac ccctagcgga gatctgaatc ctcggcgcag cacggcgagg
     1201 cccgcagccg accaacccaa cctcccgcac agccccagca cgtcccctac ctgagcaccg
     1261 gccctggtga ccggcgggcg gccctgcagg tcgccgaggg ctgtcaccgc cacgaccagc
     1321 tcagccgcgt tcaccacatc cccaccgacg atgcccacgc cgacctgcat ggcctcctcc
     1381 cacatcccgc cagcaagatc gtccatggtg gacacggggg tgcggcctgg cacggccaag
     1441 cccacgacca ggccagtagg aaccgcgccc atcgccgcta tgtcggccag gttggcggct
     1501 actgctttgc gcccgacctg gtggggcgtt gaccagtcca gccggaagtg caccccctcg
     1561 acgagcacgt cggtgctgac caccacgcga ccgtccgccg cactgagcac cgctgcgtca
     1621 tcgcctggac cgagcagcgt ggccggtggc agatcacgcg ccgaggtgat cctggcgatg
     1681 agcccgaact cgcccagacc ggctaccgag ccgacttcgg gcggcggcga gttcgaggcg
     1741 gtatcaccgg caggacccac ggctacaccc ccgatccagg gtaggtttcg ttcctactcc
     1801 gatcgagcca ccaagacgaa gggccacgcc gtggttcagg catacatcct catccagacc
     1861 gaagtcggca aggctgcagc ggtggccgca gcggtcgcca gccttcccgg cgtcgcgagc
     1921 gcggaggacg tcaccggccc ctacgacgtg atcgtgcggg ccgaggccgt cgacgtagac
     1981 cagttaggcc agctcgtggt cgccagggtg cagaacgtgg acggcatcac ccgcacgtta
     2041 acctgccccg tggtgcacct gtcctgaagg cagccgcaat ggcgcaatcg accgttaccc
     2101 cggcagccaa cctcccgcgc tggttgctcg ggatcgcagt gaccctgccg gtggcgctcg
     2161 tggtagtggt gctcgtcgtc gccgcgcagg tccggggtca gcctcagggt cccgtcgggc
     2221 tggtcagcgt cccggcaccg gcggctgact cggcggactg cacgcggctg ctggccgccc
     2281 tgcctgacga gctcgacggc ggcgagcaag gcaccctgca acagcgacaa ctggctgcta
     2341 cgaccccgtc cggcacagcc ccggctggcg cggccgcgtg gggtgagccg ccggtggtgc
     2401 tgcgctgcgg actcgaccgg cctgccgctc tgactgcgac ctcgaggctg ctgatgatct
     2461 ccggagtaca gttcctgaaa ctgcccacca ctggtacgag tacttgggtt gcagtggatc
     2521 gcccggtcta cattgtggtg gatctacccc cggtcaccgg cagcgcgccg ctgcagcagg
     2581 tcgccaccgt catagcggac atcctgcccc agcgcgacgt gaacatcccg cgctgatcgc
     2641 tacagctggt gcagcccggc cagcacccgc tccatcagcg aaaggcccgg tgggctcccg
     2701 gcctcggtcg ggttgcgatg cacgtacacc aaaccggtgt cgatcatgtc agcaagcagc
     2761 acccgagcta cccctagcgg caccgcgagc agggccgcga cctcagcaac cgagcggaca
     2821 tccacacaca gacccgcgat cgccgagtgt tctgcggagc gtgccgtggc gacgtctcgg
     2881 cctatgtcgc tggtggacac caacgtctcg acggcgagtt cctgcactgg ccgggttcga
     2941 ccttgggtcc aggtgtaagg gcgaacgatg taccggaaat cctccggccc ttcccattca
     3001 tcggccgtct cgacgaccgc cgcaccggtg ggaggctcga ctgggtcgcc ggagacctcg
     3061 gtgggctcgg tgggctcgtc agggacgtca tcgtcatcat cgtcgtctgg ttgcgcatgc
     3121 cgggcccgat agccagcccg atcccgatca cctgcgatgt cgccgagccc gtcttcgatg
     3181 agcgtcgcgt cgtgctcacc ggccggaaca ccgttgtcgt tgctttcgaa tgagggaaaa
     3241 atcgggacgc ctccccgcca gccgccgaga cccatacaca cacggcagcg accgagggac
     3301 gcagtggccc gtctccgtcc gtgcggggaa cgcccactcc gacacggacg aacccactta
     3361 ctcaactaat cattcgagaa tcggacgctg caattcaccc taatgggtgc atagcttcag
     3421 cggagtccag tcccccgggc catcgcggtg tcgatcaggg tggagagcaa agtggggtaa
     3481 tccacccccg tcaccgccca catccgggga tacatcgaga ttggtgtgaa gccgggcatc
     3541 gtgttgatct cgttgacggt caacgcgcca tcgggtccga cgaagaagtc gacccgagcc
     3601 aggccttggc agtccagcga acggaaggcg catacagcca tcgcacgcag ggcggtggtg
     3661 acgtcgtcgt cgagcttggc cgggatatcg aactcgcacg cgtcgtcgag atacttggcg
     3721 tcgaagtcgt accaacccgc ggagtctgcg gccaccacgc ggatctccgc aggcagcgat
     3781 gcttcaatcc ggccgtcggg aaactccagc acgccacatt cgatctcgcg acctgtcacc
     3841 gccgcctcga cgaggacctt aggatcgacc gcgcgagccg tggcgaccgc ggagtccagg
     3901 tcggaccagt ccaacacctt ggtgataccc gtcgacgagc ccgctctggc cggcttgacg
     3961 aacaccggca atcccagccg ctggcgctgt tccggggtca gggtggcgtg atcggagcgc
     4021 agcaccacgg catcagtgac cggcagaccc gctatgccca gcagcttgcg ggcgaactgc
     4081 ttgtccattg ccgccgcgct ggccagcacg cccgagccga catagggtag gtcggccagc
     4141 tccagcagtc cctggatcgt gccgtcctca ccgaaggccc catgcagcac ggggaacacc
     4201 acatcaaccg cggagaggac ttccccagcc cgggcgcctg cgatgcgcac cagttcccgc
     4261 ctggtcggat caccaggcaa cgcaagcgcg accgcgctac cgtctacagc gggaagcgcc
     4321 cggtcccgga tcgtta
//
LOCUS       DATADN010000825         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_705066, whole genome shotgun sequence.
ACCESSION   DATADN010000825 DATADN010000000
VERSION     DATADN010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705066"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VHH53_10715"
     CDS             complement(<1..1285)
                     /locus_tag="VHH53_10715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2290637.1"
                     /translation="MTHGELPPDLASQDLSGAGNVFRNRGFVLLWTAQTLSQLASNMV
                     LAALMATVFQATQSNTANAVLILSFLVPAVVFSAMAGVLVERSDARLIMLGTNVLRAV
                     GVIGFIFVGTNVPLILLINLFVATMTAVFAPAELTAIPRIVERRHLMAANSVFVLTIN
                     ATFAIGFGFLGPLLLVTAGADIVFVVVGVMFALAAVAIVPLPAVLPAVDQELGGKEAS
                     RALTAVFDQVREGIVFVRNHKRIAWSLGYLGIAASLIGVMGAIGPGFATDILRLSEED
                     FFFVMGPAGLGAVMGILFLNAYGKGIPRRLTIDVGLLAMGVTLIALAVVKPVVGFLAP
                     AVGPLEESLPDALSPIVSLIAVVVVIAVTAGVEYAFVAIPSQTALQEELPVNVRGRIF
                     GILNTLLSVASFLPVLVGPAVADLLNIVFPGAGIPV"
     gene            complement(1282..1500)
                     /locus_tag="VHH53_10720"
     CDS             complement(1282..1500)
                     /locus_tag="VHH53_10720"
                     /inference="COORDINATES: protein motif:HMM:NF017879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEX2290638.1"
                     /translation="MSVGAVDLRMGDVVRLRKEHPCGSREFRVVRLGADIGLVCAGCN
                     HRILMDRLDVERRFTGYVERGPEAEPPA"
     gene            complement(1497..>2025)
                     /locus_tag="VHH53_10725"
     CDS             complement(1497..>2025)
                     /locus_tag="VHH53_10725"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX2290639.1"
                     /translation="TGLPSAMDFEGVAKRRVELVTDGVATGLVHDSATARRAGVPSTG
                     HALPAPNTFGPLAWNLFMAPGSSSKDAMLSSMDRGIWVTRFHYVNIVHPRRAVLTGMT
                     KDGTFLIEGGRIVRPVRNLRFTEAIPEAFTRIEAITSATRLLGAEYTGINARVPALRI
                     GRFSFTGATAAEAGA"
BASE COUNT          362 a          703 c          700 g          260 t
ORIGIN      
        1 ccaccgggat gccggcgccg gggaaaacga tgttcagcag gtcggccacc gccgggccca
       61 cgagcaccgg caggaacgac gccacgctga gcagcgtgtt caggatgccg aagattcggc
      121 cccgcacgtt gaccggcagc tcctcctgca gggccgtctg cgaggggatg gccacgaatg
      181 cgtactcgac gcccgctgtg acggcgatga cgaccaccac ggcgatcagc gagacgatcg
      241 gcgagagggc gtccggcaga gactcctcca gcgggccgac cgccggagcc aggaacccga
      301 ccaccggttt cacgacggcc agcgcgatca gcgtgacgcc catggcaagc aggccgacgt
      361 cgatggtcag ccgccgcggg atgcccttcc cgtaagcgtt taggaacagg atgcccatca
      421 cggcgcccag cccggcaggg cccatcacga agaagaagtc ctcctcggac aggcgcagga
      481 tgtcggtcgc gaaaccggga ccgatagcgc ccatcacgcc gatgagactg gcggcaatgc
      541 cgaggtagcc gagcgaccag gcgatgcgct tgtggttgcg cacgaagacg atcccctcgc
      601 ggacctgatc gaagaccgcc gtcagcgctc ggctagcctc cttgccaccg agctcctggt
      661 cgacggccgg cagcaccgcg ggcaggggca cgatggcgac ggccgccagc gcgaacatga
      721 cacccaccac cacgaacacg atgtccgctc cggcggtgac gagcaggagg gggcccagga
      781 acccgaaccc gatcgcgaac gtggcattga tggtcagcac gaagaccgag ttggcggcca
      841 tcaggtgccg ccgctccacg atccgaggga tggccgtcag ctcggccggc gcgaagactg
      901 cggtcatcgt ggctacgaac aggttgatca gcaggatcag cggcacgttg gtgcccacga
      961 agatgaaccc gatgacgccc accgctcgca ggacgttggt gcccagcatg atcagccgcg
     1021 cgtcgctgcg ctccaccagc accccggcca tagcgctgaa caccacggcc ggcaccagga
     1081 agctcaggat caggaccgcg ttggcggtgt tcgactgggt cgcctggaag accgtggcca
     1141 tgagggccgc caggaccatg ttggacgcca gctgactgag ggtctgcgcc gtccacagca
     1201 gcacgaagcc ccggttgcgg aagacgttgc cggcgcccga gaggtcctgc gaagcgaggt
     1261 ccggcggcag ctcgccgtgc gtcacgcggg cggctccgcc tcgggccccc gttcgacata
     1321 accggtgaag cgtcgctcca cgtccaggcg gtccatcagg atccgatgat tgcagccggc
     1381 gcacaccagg ccgatgtccg cgcccaggcg gacgacccgg aactcgcggc tgccgcaggg
     1441 gtgctccttg cgcagccgga ccacgtcacc catgcgcagg tcgaccgccc ccacgctcac
     1501 gcacccgcct ccgcggccgt cgcaccggtg aacgagaagc gcccgatgcg cagggccggg
     1561 acgcgggcgt tgatgccggt gtactccgcg cccagcagcc gcgtggcgct ggtgatggcc
     1621 tcgatccggg taaaggcctc ggggattgcc tcggtgaaac gcaggttgcg cacgggacgc
     1681 acgatgcgcc ctccctcgat caggaaggtg ccgtccttcg tcatcccggt cagcaccgcg
     1741 cgccgcggat gcacgatgtt gacgtagtgg aagcgcgtga cccagatgcc acggtccatg
     1801 gagctcagca tggcgtcctt cgagctcgat ccgggcgcca tgaacaggtt ccaggccagc
     1861 gggccgaagg tgttgggtgc cgggagcgcg tggcccgtgg agggcacgcc ggcgcgccgc
     1921 gcggtggcag agtcgtgcac cagcccggtg gcgacgccgt cggtcaccag ctcgacgcga
     1981 cgcttggcca cgccctcgaa gtccatcgcg cttggcaggc cggtc
//
LOCUS       DATADN010000826         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_705075, whole genome shotgun sequence.
ACCESSION   DATADN010000826 DATADN010000000
VERSION     DATADN010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705075"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1255)
                     /locus_tag="VHH53_10730"
     CDS             complement(44..1255)
                     /locus_tag="VHH53_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX2290640.1"
                     /translation="MSLQTELPPSLIATLPGHSYTDPEIFQQEQAKIFETHWFAVARA
                     DEIEGPGSYRTVDVGRENVVLVRGRDKTLRAFLNVCRHRGARVCMEESGTGLRRLRCG
                     YHAWTYSLDGALVGAPNLAQMEDVDRSQYGLIPVHLREWLGYAWVCIADVPPSFEDTV
                     IGAVAGRLGSPEVIDDWGIDKLTVGRRITYDVKANWKLIIENFMECYHCATIHPELTE
                     VLPEFADGYAAQYFVGHGAEFGENVQGFTVDGSEGLDHIPGVDGEHDRKYYAVTVNPQ
                     VFINLVPDHVIFHRMFPVAADRTVVECDWLYMPHVVESGKDVSRSVELFHRVNEQDFD
                     AAVRCQLASNSKGYAKGGVLVPSEHHIGLFHEWVKLQLDSEHSMDAQLGAGAGHGTGL
                     ELDAGAEFGHA"
     gene            1480..>1586
                     /locus_tag="VHH53_10735"
     CDS             1480..>1586
                     /locus_tag="VHH53_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX2290641.1"
                     /translation="MPPPPRLSSDEPWALVGQLIPLQPPAVHGRTGRPGT"
BASE COUNT          260 a          537 c          527 g          262 t
ORIGIN      
        1 ggcgtggcag cccggtcgac ccgcgagcgc accggctggc ccgctacgcg tggccgaact
       61 cggcgccagc gtcgagctcg agtccggtac cgtggcccgc tccagcgccc agctgcgcgt
      121 ccatgctgtg ctcagaatcg agctgcagct tgacccactc gtggaacagg ccgatgtggt
      181 gctcactggg caccagcacc ccacccttgg cgtacccctt cgagttggac gcgagctggc
      241 acctcacagc ggcatcgaag tcctgctcgt tgacccggtg gaacagctcg accgagcggc
      301 tcacgtcttt gcccgactcg accacatgag gcatgtagag ccagtcgcac tcgacgaccg
      361 tgcggtccgc cgcgaccggg aacatccggt ggaagatcac gtggtctggc accagattga
      421 tgaacacctg agggttgacc gtgaccgcgt agtacttgcg gtcgtgctcc ccgtccacgc
      481 cagggatgtg gtcgagcccc tcgctgccgt ccacggtgaa gccctgcacg ttctcaccga
      541 actcggcgcc gtggccgacg aagtactggg cggcgtaacc gtcggcgaac tcgggcagca
      601 cctcggtcag ctcggggtgg atcgtggcgc agtggtagca ctccatgaag ttctcgatga
      661 tcagcttcca gttggccttc acgtcgtagg tgatgcgccg gccgaccgtg agcttgtcga
      721 tcccccagtc gtcgatgacc tcgggggagc ccagccgccc agccactgca ccgatgaccg
      781 tgtcctcgaa cgagggcggc acgtcggcga tacacaccca ggcgtatccg agccactccc
      841 gtagatgcac agggatcagt ccgtactgcg agcggtcgac atcctccatc tgggcgaggt
      901 tcggtgcgcc gacaagcgca ccgtcgaggg agtaggtcca cgcgtggtac ccgcaccgca
      961 gcctgcgcag gccggtcccc gactcctcca tgcacacgcg ggcgccgcgg tgccggcaga
     1021 cgttcaggaa tgcgcgcaga gtcttgtcac ggccacggac gagcaccacg ttttcccgac
     1081 ccacatccac ggtccggtac gagccggggc cttcgatctc gtccgcgcgt gcaacggcga
     1141 accaatgcgt ctcgaagatc ttcgcctgct cctgctggaa gatctccgga tccgtgtagg
     1201 agtggccggg cagcgtggcg atgagactgg gcggcagttc agtttgaagg gacacgagac
     1261 accttccgat cgcagacggt ggggcggagc gagctgcctt ctcgggtgtc ggcgctcacg
     1321 atacgccggg gctatgggtg tggcgtgacg cactctaacc agactgtgcc atctgaagca
     1381 acaagtcgcg ctatgtgaaa cagactgccg gtaggcagac gtcgcggcag caggcctcgt
     1441 gctctggccg ccccgaggtg tcggtgcgtg tcgtcgccgg tgcccccgcc gccgcgcctg
     1501 agcagtgacg agccgtgggc gctggtcggg cagctgattc cgctgcagcc gccggcggtg
     1561 cacggccgca ccggccggcc gggcac
//
LOCUS       DATADN010000827         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_243302, whole genome shotgun sequence.
ACCESSION   DATADN010000827 DATADN010000000
VERSION     DATADN010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243302"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1842)
                     /locus_tag="VHH53_10740"
     CDS             complement(<1..>1842)
                     /locus_tag="VHH53_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2290642.1"
                     /translation="CHWCHVMAHESFEDKDIARLMNANFVNIKVDREERPDVDSVYME
                     ATVAMTGHGGWPMTCFLTPTGEPFHCGTYYPPAARHGLPGFPELLLAVRQAWTNRGDE
                     VREAAKRVVGQLTQRAVPLTESAVDDRVLATAVDKLAGEFDHQHAGFGGAPKFPPSML
                     LEFLLRHHERTGSEPALSMAQKTCEAMARGGMYDQLAGGFARYCVDAAWVVPHFEKML
                     YDNALLLRVYTHLTRLTGSVTARRVTEQTATFLLKELGTPQGGFAASLDADTDGHEGA
                     TYVWTPQQLVEVLGPDDGPWAAKLLRVTPTGTFEHGTSVLQLPADPDDADRWERVRTV
                     LLAARAARSQPARDDKVVTEWNGMTITTLAEAGGVLDRPEWTAAAAAAAELLLGTHIV
                     DGRLRRSSRDGVVGAAAGVLADYAWLIEGVLALHQATGKDRWLHAATPLLDQVQKHFA
                     DPEHAGAWFDTAADAEALFRRPANPTDNATPAGASALAGALVTVSALTGSEFYRAAAE
                     AAVARAGLVLAKAPQAAGHWLTTAEALAHGPVQIAVIGPAGDAARAALEVKARWAAPG
                     GAVVLVGEPGAEHPMLAGRDLIAGAPAAYVCRGFVCDRPATSLDDLQG"
BASE COUNT          293 a          647 c          615 g          287 t
ORIGIN      
        1 gccttggaga tcgtccaggg aggtcgctgg gcggtcacag acgaacccac ggcagacgta
       61 ggccgctgga gcccccgcga tcaggtcccg tccggcgagc atcgggtgct cggcacccgg
      121 ttcgccgacc agcacaacgg ctccgccggg tgcggcccac cgggccttga cttccaacgc
      181 tgcgcgggcc gcatccccgg ccggaccgat cacagcgatc tgcaccggcc catgtgccag
      241 cgcctcagcg gtggtcagcc agtgcccagc cgcctgtggt gccttggcca gcaccaatcc
      301 ggcccgcgcc accgcagctt cggccgccgc gcggtagaac tccgaaccgg tcagcgctga
      361 gacggtgacc agcgcgccgg ccagcgcgga ggcgcccgcc ggggtggcat tgtcggtcgg
      421 gttggcgggg cggcggaaca acgcctcggc atccgcggcg gtatcgaacc aggcaccggc
      481 atgttccgga tcggcgaaat gcttctggac ctggtccagt aacggcgtcg cggcgtgcag
      541 ccatcgatcc ttcccggtgg cctggtgcaa cgcaagcaca ccttcgatca gccaggcgta
      601 gtcggccagc accccggcgg cggcaccgac cacaccgtca cgcgaactgc gccgcagcct
      661 gccatcgacg atgtgcgtgc ccagtagcag ctccgctgca gcagccgccg ccgcggtcca
      721 ctccggacgg tccaggacgc caccggcctc ggccagcgtg gtgatcgtca tcccgttcca
      781 ctcggtgacc accttgtcgt cccgcgcggg ctgtgagcga gcagcccggg cagcgagcaa
      841 cacggtgcgg acccgctccc aacggtcggc gtcatcggga tcggcgggca actgcaacac
      901 cgaggtgccg tgctcgaatg tccccgtcgg ggtaaccctc agtagcttgg ctgcccacgg
      961 accgtcatcg ggtccgagca cctcgacgag ctgctgtggc gtccacacgt aggtggcgcc
     1021 ctcgtggcca tcggtgtcgg cgtccagcga tgccgcaaag ccgccttgcg gggtacccag
     1081 ctccttgagt aggaacgtcg cggtctgctc ggtcacccgc cgggcggtca ctgagcctgt
     1141 cagccgggtc agatgggtgt agacgcgcag cagtaacgcg ttgtcataga gcatcttctc
     1201 gaagtgtggc accacccatg cggcatcgac gcagtaacgg gcgaacccac cggcaagctg
     1261 gtcatacatc ccaccgcggg ccatcgcttc acatgttttc tgcgccatgg acagcgccgg
     1321 ctccgagccg gtccgctcgt gatgccggag caagaattcc agcagcatcg atggcgggaa
     1381 cttcggggca ccaccgaagc cggcgtgctg gtggtcgaac tcccccgcca gcttgtcgac
     1441 cgccgtggcc agcactcggt cgtccactgc ggattcagtc agcggcacgg cccgttgggt
     1501 gagctgcccg acgacgcgct tggcggcctc ccgtacctcg tcaccacggt tggtccaggc
     1561 ctgccggaca gcgagtagca gctcgggaaa gcctggcagg ccgtgccgag ccgccggagg
     1621 gtagtaggtg ccgcagtgga acggctcgcc ggtcggtgtc aggaagcagg tcatcggcca
     1681 tccaccgtgg ccggtcattg caacggtcgc ctccatgtag acggagtcga cgtcggggcg
     1741 ctcctcccga tccaccttga tgttgacgaa gttggcgttc atcaggcgag cgatgtcctt
     1801 gtcctcgaag gactcgtgtg ccatcacgtg gcaccagtgg ca
//
LOCUS       DATADN010000828         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1165299, whole genome shotgun sequence.
ACCESSION   DATADN010000828 DATADN010000000
VERSION     DATADN010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165299"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..>1635
                     /locus_tag="VHH53_10745"
     CDS             63..>1635
                     /locus_tag="VHH53_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX2290643.1"
                     /translation="MATRPPIGLPKLSRRSRILLIIAATVILLLLLGARLLDTYVDWL
                     WFGEVGYRSVFSTVLLTRIGLFFGVGLLVGGLLAVSLIIAYRTRPVFVPVSGADDPLA
                     KYRSTVMQRVRLFGIGIPVIVGLIAGSTAQTDWQQFQLLLNGTPFGITDPEFGKDIGF
                     YAFTLPFITWLISWLFVSIAIAFFGALITHYLFGGVRLVGRGGQFSVPARMQLSITAG
                     LFVVVYAVDYFFERYELLFSDRNDLFTGATYTDLNAVLPAKLILMFIAGFCAVAFFVG
                     AFLRNLQLPAIATALLVLTSILIGAAWPAVLEQFSVRPNANEKEAKSIERNIAATRLA
                     FGLTDDKVKFSDYSGEPNATGPEVRNNPSNVETIENLRLLDPSILSPTFTQREGRENF
                     YGFPEKLDIDRYTVDGETRDYIVAAREIRTEGLAENQRNWINQHLVFTHGNGFVAAPA
                     NTVEALQEASGQGGYPIFSVSDLTNRGSIRVDQPRVYYGELSTNYAIVGGQTGEAPGE
                     YDTPTDRSFIYDGKGG"
BASE COUNT          264 a          565 c          505 g          301 t
ORIGIN      
        1 catctagggc agtcgtgcgc ggctgctcga cgacttgacg acgcgacaca ggagcgtgcc
       61 ccgtggccac tcggcccccg atcggcttgc ccaagctttc ccgacgcagt cgcatcctgc
      121 tgatcatcgc ggcgacagtg attctgttgt tgctcctcgg cgcccgcctg ctcgacacct
      181 acgtcgactg gctgtggttc ggtgaggttg gttaccgctc ggtgttcagc accgtgctgc
      241 tcacccggat cgggttgttc ttcggcgtcg gcctgctggt cggcggcctg ctcgcggtca
      301 gcctgatcat cgcctaccgg acgcggccgg tgttcgtacc ggtgtccggc gccgacgatc
      361 cactggccaa gtaccgctcc acggtcatgc agcgggtgcg gctgttcggc atcggcatcc
      421 cggtcatcgt cggcctgatc gccggctcga ccgcgcagac cgactggcaa cagttccagc
      481 tactgctcaa cgggaccccg ttcggcatca ccgatcccga gttcggcaag gacatcgggt
      541 tctacgcgtt cacgctgccg ttcattacct ggctgatcag ctggctgttc gtctcgatcg
      601 cgatcgcctt cttcggagcg ctgatcacgc attacctgtt cggtggcgtg cggctggtgg
      661 gtcgcggcgg gcagttctcg gtgccggcca ggatgcagct gtcgattacc gcgggcctgt
      721 tcgtcgtggt gtacgcggtc gactacttct tcgagcgcta cgagctgctg ttctccgacc
      781 gcaacgacct gttcaccggc gccacctaca ccgacctcaa cgcggtcctg ccggccaagc
      841 tcatcctgat gttcatcgcc gggttctgtg ccgtggcctt cttcgtcggg gcgttcctgc
      901 gcaacctgca actgccggcc atcgccaccg cgctgctggt gctgaccagc attctcatcg
      961 gggccgcctg gccggccgtg ctcgaacagt tctcggtgcg gccgaacgcc aacgagaagg
     1021 aagccaagtc gatcgaacgc aacatcgcgg caaccaggct tgccttcggg ctcaccgatg
     1081 acaaggtgaa gttcagcgac tacagcggcg agcccaacgc caccggtccc gaggtgcgca
     1141 acaaccccag caacgtcgag accatcgaga acctgcggtt gctcgacccg agcatcctgt
     1201 cgcccacctt cacccagcgc gagggacggg agaacttcta cgggttcccc gagaagctcg
     1261 acatcgaccg gtacaccgtc gacggcgaga cgcgggacta catcgtcgcc gcccgggaga
     1321 ttcgtaccga ggggctggcc gagaaccagc gcaactggat caaccagcac ctggtcttca
     1381 cgcacggcaa cggatttgtc gccgccccgg ccaacaccgt cgaggcgttg caggaggcga
     1441 gcggccaggg tggctacccg atcttctcgg tgagcgatct caccaatcgc ggcagcatcc
     1501 gggtcgacca gccccgcgtg tactacgggg agctgtccac gaactacgcc atcgtcggcg
     1561 ggcagaccgg cgaggcaccc ggtgagtacg acacccccac cgaccgcagc ttcatttacg
     1621 acggcaaggg cggcg
//
LOCUS       DATADN010000829         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_86828, whole genome shotgun sequence.
ACCESSION   DATADN010000829 DATADN010000000
VERSION     DATADN010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86828"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VHH53_10750"
     CDS             complement(<1..350)
                     /locus_tag="VHH53_10750"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2290644.1"
                     /translation="MAGPETRNVVVAGPAGVGKTRLAREALEAAKRSGRATRWAAGTS
                     AAAAIPLGAVAHLLPALGTVSDPLTLLQRAGAAIADQGKGHRLIVGIDDAHLLDELSV
                     TLVHQLALTRSACLV"
     gene            complement(743..1621)
                     /locus_tag="VHH53_10755"
     CDS             complement(743..1621)
                     /locus_tag="VHH53_10755"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX2290645.1"
                     /translation="MTATVGADRGLLRAAGAALRAAAVLTAVMLLTGCMGSGTSGTSG
                     TGAGDSAGNGYGGRGGNGYGGDGGSAEGDTGSVVGSGRLTSRLMSLSGVNSVVVGAGF
                     VVRLDIGGPEQARITMDDNLTDQVQVTVTGGELRLGLAPGTHVRNATLTAEITVARMD
                     RLATNGVSRVVLVSAPAGPALDLIATGTSEVTGPIRVDRLQATASGAATLALSGQARD
                     LHLSGAGTSRLLLADLVVRNLDAELSGTSHATVTVSDTLAAQTTGVSALRYRGSPRVT
                     RAQTSGVSSIARDGFK"
     gene            complement(1621..>1843)
                     /locus_tag="VHH53_10760"
     CDS             complement(1621..>1843)
                     /locus_tag="VHH53_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290646.1"
                     /translation="FPVVWDRLLQLGSLTGAAAVDGSVAEATLGAINIVLLLLPWVGG
                     VLLFCMIMHGPLRWVLGRWGPARLRVRRS"
BASE COUNT          366 a          669 c          523 g          285 t
ORIGIN      
        1 accagacacg ctgagcgggt cagggccagc tggtgcacca gggtcaccga cagctcatca
       61 agcagatggg catcgtcgat ccccacgatc agccggtgtc ccttgccttg atcggcgatc
      121 gcggcacccg cgcgctgcag caacgtcaac gggtcggaca cggtccccaa agccggcagc
      181 agatgtgcca ccgcccccaa cgggatcgcc gccgccgcac tcgtccctgc cgcccagcgg
      241 gttgcacgac cgctgcgctt ggccgcctcc aacgcctccc gagcaagccg cgtcttgccg
      301 acccctgcgg gaccggcaac gaccacatta cgtgtttccg gaccggccat acgctccgtt
      361 ataaaccgca actcagagtc acggcccatg agcgtccaag aatctgctct catagctgaa
      421 ccggccaacg cacttggtat cccgcgctga tccgggatgc aactgacggc ccggcacaaa
      481 aagccgaccg ctcctgcccc ctctgacaca ttggctacgc ccccccgcgt cacgccaata
      541 agctgacgag ctacccccag caatcagcat atgctaaccg tgacgagatc gccacggtcg
      601 atacgtccaa gaaatatgaa taaccatgga tgtcgggata tgcggctcga caccgtaaca
      661 gtgcgtgatc aagtggtgac gtcctgcttg gctttcgtcg gcgtcgattg atttgggccg
      721 atcacctaga atagttgggc aattacttaa atccatcccg ggcgatcgac gacaccccgg
      781 aggtttgcgc gcgggtgacc ctcgggctgc cccggtagcg cagcgctgag acacccgtgg
      841 tctgtgcggc cagggtgtcg ctgacggtca cagtggcatg gctggtcccg gacagctcag
      901 cgtcgaggtt tcgaacaacc aggtcggcca gaagtagccg actggtcccc gccccactga
      961 gatgaaggtc tctcgcctgt ccggacagcg ccagggtggc ggccccggac gcagtggctt
     1021 gcaggcgatc gacacggatg ggaccggtga cctcgctggt cccagtagcg atcaggtcca
     1081 gtgccgggcc tgccggcgcc gagaccagca cgactcggct gacaccgtta gtagcaagcc
     1141 gatccatacg ggccacggtg atctcggcgg tcagggtggc attgcggacg tgggtgcctg
     1201 gagcgagccc tagccggagc tcaccaccgg tcaccgtgac ctggacctga tcagtgagat
     1261 tgtcatccat cgtaatcctc gcctgctcag gcccgccgat gtcgagacgg actacaaaac
     1321 cggctccgac taccactgag ttgacaccgg acaagctcat cagccggctt gtgagacgcc
     1381 cagagccaac taccgagccg gtatccccct ctgcgctgcc cccgtcaccg ccgtatccat
     1441 tgccgccccg accgccataa ccattgccgg cgctatcccc ggcgcccgtg ccgctggtgc
     1501 cgctggtgcc gctgcccata cagccggtca gcaacatcac tgcggtcagc accgccgccg
     1561 cccgcaacgc agcgccagcc gctcgcagta ggccacggtc tgcgccgacg gtggcagtca
     1621 ctaggacctc ctcacccgca gccgggccgg tccccagcgg cccaacaccc accgcaacgg
     1681 accatgcatg atcatgcaga acaacagcac accacccacc cagggcagca acaacaacac
     1741 aatgttgatt gcacccaacg tggcctcggc cacgctgcca tcgactgccg ccgcaccggt
     1801 cagcgatccc aactgcagca accggtccca caccaccgga aac
//
LOCUS       DATADN010000830         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_558812, whole genome shotgun sequence.
ACCESSION   DATADN010000830 DATADN010000000
VERSION     DATADN010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558812"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VHH53_10765"
     CDS             <1..438
                     /locus_tag="VHH53_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEX2290647.1"
                     /translation="VYGMAGAHRHTPEIIQNLTSVAGRAVTVSFTPVLAPMPRGILAS
                     CIATVLPGTDHETARSVYHAAYTAEPFVHLLAPGRWPATAAVQGSNSIQLQVAVDTDA
                     GRLVAVGAVDNLTKGTAGAAVQCFNLALGLPETTGLPAWGIAP"
     gene            470..1627
                     /gene="argJ"
                     /locus_tag="VHH53_10770"
     CDS             470..1627
                     /gene="argJ"
                     /locus_tag="VHH53_10770"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX2290648.1"
                     /translation="MSVTAAKGFRAAGVAAGIKISGGLDLALVVNDGPSRAAAGMFTR
                     NQVKAAPVLWSAQVLTGRRLRAVALNSGGANACTGPSGFQDTHVTAERVATVLDCGAI
                     EVAVCSTGLIGERLPIDAVLSGVDSAAAALAATPEAGLAAATAVLTTDTVPKQAAIAG
                     TGWTVGGFAKGAGMVAPGLATMLCVLTTDAAVDDVTLDAVLRGATAETFERLDIDGTC
                     STNDTVLLLASGASGVMPDRAEFATTVQRVCHQLVKSLQADAEGVTKEIAITVCGAAS
                     DADAILAARAVARDSLVKTAFFGSDANWGRIAAAVGASAATVTAGTLDIRINGLLLCQ
                     GGTVAADRTKADLSGRYITVEIDLHLGEGQATVLTTDLSHGYVDENSAYSS"
     gene            1624..>1731
                     /locus_tag="VHH53_10775"
     CDS             1624..>1731
                     /locus_tag="VHH53_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX2290649.1"
                     /translation="MTTVGIDAAAQKAAVLAEALPWLQRFHGTTFVIKYG"
BASE COUNT          278 a          597 c          596 g          260 t
ORIGIN      
        1 gtctacggga tggccggcgc gcaccggcac accccggaga tcatccagaa cctcacctcc
       61 gtcgccggcc gggcggtcac cgtgtcgttc acaccggtgc tagcgccgat gccgagaggg
      121 attctggcca gttgcatcgc aacggtgctc ccaggcaccg accacgagac ggctcgcagc
      181 gtataccacg ccgcatatac tgcggagcca ttcgtgcacc tgcttgcgcc gggccgttgg
      241 ccggccaccg ctgccgtgca gggttccaac tccatccagt tgcaggtcgc ggtggacacc
      301 gacgccggtc ggctggttgc agtgggcgcg gtggacaacc tcaccaaagg cactgcggga
      361 gcggctgtcc agtgcttcaa cctggcgttg ggacttcccg agaccacggg cctgccggcc
      421 tggggaatcg cgccctgata cgtcaggcac cggccgggag gacatcgcga tgagcgtcac
      481 tgcagccaag gggttcaggg cggccggcgt agccgctggt atcaagatca gcggtggtct
      541 ggacctggct ctggtggtca atgacggacc gtcgagggcg gccgcgggga tgtttacccg
      601 caatcaggtc aaggcggcgc cggtgctgtg gtcggcgcag gtcctcaccg ggcgacggct
      661 gcgcgcagtc gcgctgaact ccggtggcgc caacgcgtgc accggtccaa gcgggttcca
      721 ggacacccat gtcactgcag agcgggtcgc tacggtgctc gactgtggag cgatcgaggt
      781 cgcggtctgc tcgaccgggc tgatcggcga gcggttaccg atagacgccg tgctctccgg
      841 agtcgacagc gccgccgcag cactggccgc gacgccagag gccggcctgg cggccgccac
      901 tgcagtgctc accaccgaca ccgtgcccaa gcaggccgcc atcgcgggca cgggctggac
      961 ggtaggtggg ttcgccaagg gagccggcat ggtcgcgccc ggcttggcca ccatgctgtg
     1021 cgtactcacc accgacgcag ccgtcgatga cgtcaccttg gacgccgtgc tgcgcggcgc
     1081 cacggccgag accttcgagc gcctggacat cgatggcacc tgctcgacca acgacaccgt
     1141 gctcttgctc gcgtcgggcg catcgggcgt gatgccagac cgtgcggagt tcgccaccac
     1201 ggtgcagcgg gtgtgccatc agctggtcaa gagcttgcag gccgatgccg agggcgtcac
     1261 caaggagatc gcgatcaccg tctgcggcgc ggccagcgac gccgacgcca tcctcgcggc
     1321 ccgcgcggtc gcccgggata gcctggtcaa gacagccttc ttcggttccg acgccaactg
     1381 gggacggatc gccgccgcgg tgggcgcctc cgcagccacc gtcaccgccg gcacgctcga
     1441 catccggatc aacggactcc tgctctgcca aggcggcacg gtggcggccg accgcacgaa
     1501 ggcggatctg tccggccggt acatcaccgt cgagatcgat ctgcacctcg gcgagggcca
     1561 agccaccgtg ctcaccacgg acctttccca cgggtacgtc gacgagaaca gcgcgtactc
     1621 gtcatgacga cagtgggcat cgatgcagcg gcgcagaagg ccgcggtgct cgccgaggcg
     1681 ttgccgtggc tgcagcgctt ccacgggacc accttcgtga tcaagtacgg c
//
LOCUS       DATADN010000831         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_243520, whole genome shotgun sequence.
ACCESSION   DATADN010000831 DATADN010000000
VERSION     DATADN010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243520"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VHH53_10780"
     CDS             <1..734
                     /locus_tag="VHH53_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2290650.1"
                     /translation="RWGLLLARTNPDLPKHKGLTYFVLDMHAPGVETRPLRQMTGHAE
                     FNEVYMSDARIPDAHRLGEVGDGWRVAMTTLMNERSALGASGNRRGAGTIADAVSLWA
                     ARPDLRTPVLRDRLSQLWLRAEAQRLTSERSRATATVGGPGPEGSVGKLVGAELNQHI
                     YEFCMDLLGPEGILYHSYAMRDSDREVEDWRGPLQQRFLRSRANTIEGGTSEVMRNIL
                     GERVLGLPGDLRADAGMPWKEIPRG"
     gene            727..1875
                     /locus_tag="VHH53_10785"
     CDS             727..1875
                     /locus_tag="VHH53_10785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2290651.1"
                     /translation="MAEFAFTDEQAQLRSAVRKFCAENYDEQTVRRLMESDPPFDAKV
                     WARLGAELGVLGLSVPEADGGVGGSLVDQAVAVEELGASLACGPLFGTVFLAIPALVA
                     ASAGAGRDELLADLVEGRRTAAFAVGDRAGAFDASAVTVTASGDTVSGTVEQVVDAGA
                     ADVLLVAATGPDGVGLYAVDGTDAERTPLVTLDLTRPQATIRFSETPARLVTGPAEAE
                     RVISHALQVGSALLAVEQVGAAQHLLDLAVAYAKERLQFGRPIGSFQAVKHRLADMLV
                     DLEHARSTAYHAVWALTDGSDDPALAVSIAQATCSAALLHIAKDTIQVHGGIGFTWEH
                     QAHLYFKRASTAAALLGNAEQHRDRVASLVLDTASADRVPRIATGAPA"
BASE COUNT          295 a          625 c          691 g          287 t
ORIGIN      
        1 cgcgatgggg cctgctgctg gcgcgcacca accccgatct gccgaagcac aaggggctca
       61 cctatttcgt cctggacatg cacgccccgg gcgtcgaaac caggccgctg cgccagatga
      121 cgggccacgc cgagttcaac gaggtgtaca tgtcggatgc gcggataccc gacgcgcatc
      181 ggctcggcga ggtcggcgac ggctggcggg tggcgatgac caccctgatg aacgagcgca
      241 gtgcgctcgg cgcgagcggc aaccggcgcg gggcgggcac gatcgccgac gcggtgtcgc
      301 tgtgggcggc tcggcccgat ctgcgcacgc ccgtgctgcg cgaccggctg agccagctct
      361 ggctgcgcgc cgaggcgcag cggctgacct ccgagcgctc gcgcgccacc gcgaccgtcg
      421 gcggtcccgg ccccgaaggc tcggtcggca agctggtggg cgccgaactg aaccagcaca
      481 tctacgagtt ctgtatggat ctgcttgggc ccgaaggcat cctgtatcac agctacgcga
      541 tgcgtgacag tgaccgcgag gtcgaggact ggcgcggccc gttgcagcag cggttcctgc
      601 gcagccgcgc caacacgatc gagggcggca catccgaggt gatgcgcaac atcctcggtg
      661 aacgagtgct cggattaccg ggtgacctgc gtgccgatgc cggcatgccg tggaaggaga
      721 ttccccgtgg ctgagttcgc gttcaccgat gaacaggcgc agctgcgcag cgcagtccga
      781 aagttctgcg ccgagaacta cgacgagcag accgtgcgac ggctgatgga atccgatcca
      841 ccgttcgacg cgaaggtgtg ggcaaggctg ggtgcggaac tcggcgtgct cgggctgtcg
      901 gtgcccgagg ccgacggcgg tgtcggtggc agcctggtcg accaggcggt ggcggtggag
      961 gaactgggtg cgtcgctggc ctgcgggccg ctgttcggaa ccgtgtttct ggccatcccg
     1021 gcgctcgtcg cggcatcggc aggcgccggg cgtgacgaac tgctcgccga cctggtcgag
     1081 ggcaggcgca ccgcagcgtt cgccgtcggt gaccgagcgg gcgctttcga cgcctccgct
     1141 gtgacggtga ccgcgtcggg agacaccgtg tccggaacgg tcgagcaagt cgtcgacgcg
     1201 ggcgcggcag atgtgctgct ggtagccgcg accgggcccg acggcgtcgg actgtacgcc
     1261 gtcgacggca ccgacgcgga acgcacgccg ttggtgacac ttgacctgac ccgtccgcaa
     1321 gcgaccattc gcttctccga aacgccagcc cggctcgtca ccgggcccgc ggaggccgaa
     1381 agggtcattt cccatgcgct gcaagtgggt tcggcactgc tggccgtcga gcaggtcggc
     1441 gccgcacagc acctgctcga cctggcagtg gcctatgcca aggagcggct gcaattcggc
     1501 aggcccatcg gatcgtttca ggcggtcaag caccggcttg cggacatgct ggttgacctc
     1561 gagcacgccc ggtcgactgc ctaccacgct gtctgggcgc tgaccgacgg gtcggatgac
     1621 cccgcgctcg cggtcagcat cgcgcaggca acctgctcgg cggcgctgtt gcacatcgcg
     1681 aaggacacca tccaggtgca cggcggtatc ggattcacgt gggaacacca ggcgcacttg
     1741 tacttcaagc gggcgagcac cgctgcggcg ctgctgggca acgcggagca gcatcgggac
     1801 cgggtggcgt cgctggtgct cgacaccgcg tcggccgacc gggtgccgcg gatcgcgacg
     1861 ggggcacccg cctagccgtt ccgcgagtgt gaagctgt
//
LOCUS       DATADN010000832         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1015227, whole genome shotgun sequence.
ACCESSION   DATADN010000832 DATADN010000000
VERSION     DATADN010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015227"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            713..1144
                     /locus_tag="VHH53_10790"
     CDS             713..1144
                     /locus_tag="VHH53_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290652.1"
                     /translation="MSLRQALPAGDNETTLTHVVVDGVLVRTIALTLTPTQRARLQGA
                     RTPGPTPLLDQRPARVQRTVSCRGGTQIIGQRVQVGLRYAGQIVTIEIDETTLRVYDQ
                     RNHLIKHVPRTSRKEVHRHKAYGHTTKQKIGSSQQSLGAGL"
     gene            complement(1139..1474)
                     /locus_tag="VHH53_10795"
     CDS             complement(1139..1474)
                     /locus_tag="VHH53_10795"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2290653.1"
                     /translation="MRGTEILMRGRVGDDLTVEDGYASARLTALAIISSLRQVVGTLD
                     ALTWLRATIYVNAVPDLDGPALTRVADGFSDVVNDLFGEWGQHARATCGVSALAFGVP
                     TIIESVVAL"
     gene            1663..>2136
                     /locus_tag="VHH53_10800"
     CDS             1663..>2136
                     /locus_tag="VHH53_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2290654.1"
                     /translation="MSRAERTRRRILDAAGALLQARPGTLPSMSDVAAAAGVTRQLLY
                     VHFDNRTALFLELSRLIDAEASTPELQATIDNAPDARAALRAAVRVQATIKPKLHGIA
                     ASLELLRPVDEGAAGACQEREDARLMRCRAVTDRLAAEGWLAPGWTSVEAAELLWS"
BASE COUNT          394 a          724 c          671 g          347 t
ORIGIN      
        1 aattagctcc tctatatcgc gacctcgcaa atggcagccg tggtggctgc ccgtgatact
       61 ggttttcttc accggcagag agtagccgat ggccagagag acgctaaacg gtccggacag
      121 gttgagtact cccgaagttg caatcatcga ccgaggggga gggagcgcgt gttaaccacg
      181 gcaggatctt ccaccacccg actcggccgc acctcgacgc tcgacctatc gcggacgctg
      241 gagtcggccg ctgccgcgct cggtgatcac gccgccccga gcacgcggtc ctctcttggg
      301 tcgccgcagt gtccaatgtc ggggtgtcca atgactgtca atactcgcgg cactgcccgt
      361 aactagctcg cagcacagga ggcgcgatgt gggtgcacct gaccggactt ccgcggtcgt
      421 cgactgtggg atcaagggta actggcttgg gggtagcccc tgcttcaagt gtcgtatcga
      481 cggggcggga cgtagtgtgc tcggcatggc cgcagacccg aaagacgcac tggatccgga
      541 cagctctaaa ttcctcaacg agcaagaccc tgtcgagaag gtgagcccgg agacgacggc
      601 ggacgctccc gccacgcagg ccgagagcat ctcgaccgac ccctcaccga gcgagggcga
      661 acagcccaga acctaactac gtcatcgcgc gaatgaaggg agagggaggt gcgtgtcact
      721 gcggcaggcc ctgcctgccg gcgataacga gaccaccctc acccacgtcg tcgtggacgg
      781 tgtcctggtc cggaccatcg cgctgaccct gacccccact caacgcgccc gtcttcaggg
      841 cgcccgcacc cctggcccta ccccgttgct agaccagcgc cccgcccggg tgcagcgcac
      901 agtgtcctgc cgcggcggca cccaaatcat cggccaacgc gtgcaagtcg ggctccgtta
      961 tgccggtcag atcgtgacca tcgaaattga cgagaccacc ctgcgggtct acgaccagcg
     1021 caaccacctg atcaagcatg ttccccgcac cagccgcaag gaggtccacc gacacaaggc
     1081 ctacggtcac accaccaaac agaaaattgg ttcttcgcag caaagcttgg gtgcggggct
     1141 atagggcgac caccgactcg atgatggtgg gcacgccgaa cgcgagcgca ctaacaccgc
     1201 atgtggctcg tgcgtgctgg ccccattccc cgaagaggtc gttgaccacg tcgctgaacc
     1261 cgtctgccac gcgtgtcagc gcgggtccgt caaggtcggg cacggcgttg acatagatgg
     1321 tcgcccgtag ccaggtgagg gcgtcgaggg ttccgaccac ctgccgcagt gaggaaatga
     1381 tcgcaagcgc ggtcagtcgg gctgatgcat atccgtcctc tacggtgagg tcgtcaccga
     1441 cccggccgcg catgagaatc tcggtgccgc gcacgggacc gtgcccggcg acatagccga
     1501 ttccgccgtg gacacggacg atttggaagg tctgtcgaac ctgttccggg agccgccacg
     1561 gctcgggtag ctcgacgccc agggtggagc cggtccaggg tgcgtgtcat gcctacctcc
     1621 tttgacagcc tgtcaaaaaa tcaaagctga tattttgaca ccgtgtcaag agctgagcgg
     1681 accaggcggc ggatcctcga tgctgccggg gctctcctcc aggcccggcc tgggacgctc
     1741 ccgtcgatgt ccgacgtcgc ggcggcggcc ggcgtcactc gtcaactgct gtatgttcac
     1801 ttcgacaacc gcaccgcgct attcctcgaa ctcagccggc tcatcgacgc cgaggccagc
     1861 acccccgaac tgcaggccac catcgacaac gcgcccgatg ctcgcgccgc gttgcgcgcc
     1921 gctgtccgcg tgcaggcgac catcaagcca aagctccacg gcatcgccgc cagccttgag
     1981 ttactgcgac ctgtcgacga gggcgccgcc ggagcctgtc aggagcgcga ggatgcccgc
     2041 ctgatgcgct gccgtgcggt gaccgaccgc ctcgcggccg agggctggct ggcgcccggc
     2101 tggaccagcg tcgaggccgc cgagctgctc tggtcg
//
LOCUS       DATADN010000833         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1165698, whole genome shotgun sequence.
ACCESSION   DATADN010000833 DATADN010000000
VERSION     DATADN010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165698"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="VHH53_10805"
     CDS             <1..1371
                     /locus_tag="VHH53_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HEX2290655.1"
                     /translation="DLGYAGVALTLDHHHLNPFGPGLARRIAAVAGRLSELGLAVVVE
                     TGARYLLDPWTKHHPTLVSDGWPRRVDFLRRAIRIAEDLGAECVSLWSGALPPGTDPG
                     LGRAWLIDGMAQVVEEAAARGVALGLEPEPGHVIERLSDALALRAALGTPELLGITLD
                     VGHCVAVELDDAATCLRRAGALLVNVQLDDMRRGVHEHLEFGEGELDLPATLAALAEI
                     GYAGLASVELPRHGHAAPQVARRALTALHQALPVGMQAGPLGWLDRAIEEIRNDPVRI
                     RALLPTAGREVGRGPLRPQQDPQGLVHGTVDDAARGRLLRALSPRALAAELPDLYRYG
                     DDAEKRGVLRALPGLDIAPELGLPLVLDALRTNDIRLVAAAMGQYAAAHLDQHSWRHG
                     VLKCVFVGVPLDAVARCSERVDAELRRMVTDYAEERRAAGRVVPGDVHRLLGGARCGS
                     STRTST"
     gene            1341..>1517
                     /locus_tag="VHH53_10810"
     CDS             1341..>1517
                     /locus_tag="VHH53_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase TatD"
                     /protein_id="HEX2290656.1"
                     /translation="MRIFDPHIHMTSRTTDDYEAMAAAVVRAVVEPSFWLGQPRTSVG
                     TFVDYFDSLLGWERF"
BASE COUNT          192 a          513 c          563 g          249 t
ORIGIN      
        1 gaccttggct acgccggggt ggcgctgacc ctggatcatc atcacctcaa cccgttcggc
       61 cccggcttgg cccggcgcat cgccgcggtg gccggccggc tgtccgagct gggccttgct
      121 gtggtggtgg agaccggggc acggtacctg ctcgatccgt ggaccaagca tcatccgacc
      181 ctggtgtccg atgggtggcc ccggcgggtc gattttctgc gccgtgccat ccgcattgcc
      241 gaggatctgg gtgcggaatg tgtgtccttg tggtccggtg ccctgccgcc aggcaccgac
      301 cccgggctcg gccgcgcctg gctgatcgac ggaatggctc aggtagtcga ggaggctgcg
      361 gcgcgcggcg tggcgctggg tctggagcct gagccggggc atgtcataga gcgcctcagc
      421 gatgccctgg cgctgcgcgc ggcgctgggg acacccgagc tgctgggaat cacgctcgac
      481 gtcgggcact gcgtggcggt ggagctcgac gatgctgcca cctgcctgcg ccgagctggg
      541 gcactgttgg tcaacgtgca gctcgacgac atgcgccgtg gcgtgcatga acaccttgaa
      601 ttcggggaag gagagctgga cctgcccgcc accctggccg cgctggccga gatcggatac
      661 gccggtctgg cctcggtgga gctgccccgg cacggccacg ccgcgccgca ggtcgctcgc
      721 cgggccctga ccgcacttca ccaggctctt cccgtgggga tgcaggccgg gccgctgggc
      781 tggctggacc gcgccatcga ggagatccgg aacgatcccg tccgcatccg ggcgctgctc
      841 ccgaccgcgg gccgggaggt gggccgtggc ccgctgcgcc cgcagcagga cccgcagggt
      901 ctggtgcacg gtaccgtcga cgatgccgcc cgcgggcggt tactgcgggc actgtcaccc
      961 cgggcgctcg ccgccgagct tcccgacctg taccggtacg gcgatgatgc ggaaaagcgc
     1021 ggggtgctgc gtgcgctgcc cggtcttgat atcgcaccag agctcgggct gccgctggtg
     1081 ctggatgcgc tgcggaccaa tgacatccgg ttggtcgccg cggcgatggg gcagtacgcg
     1141 gcagcgcacc tggatcagca ttcctggcgg catggggtgc tcaagtgcgt gttcgtcggg
     1201 gtgccgctgg acgcggtcgc gcgctgcagc gagcgtgtcg acgccgagct tcgccggatg
     1261 gtcactgact acgccgagga gcgccgcgct gccgggcgcg tggtgcctgg cgacgtgcac
     1321 cgattgctag gaggagccag atgcggatct tcgacccgca catccacatg acctcgcgca
     1381 ccaccgacga ctacgaggcg atggccgccg ccgtggtgcg cgccgtcgtc gagccgtcgt
     1441 tctggctcgg ccagccgcgc accagcgtcg gcacgttcgt ggactacttc gacagcctcc
     1501 tgggctggga gcggttc
//
LOCUS       DATADN010000834         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_412627, whole genome shotgun sequence.
ACCESSION   DATADN010000834 DATADN010000000
VERSION     DATADN010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412627"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..698)
                     /locus_tag="VHH53_10815"
     CDS             complement(195..698)
                     /locus_tag="VHH53_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5130 family protein"
                     /protein_id="HEX2290657.1"
                     /translation="MANGDEEHGKPARRHQTPHDSDQQERALGEVVVGTGRISTARQV
                     RPVLPTLPFTKSQRAALDDALVCASRSVGLHFGVYLGDLGPDTRARAEELHELMGGRA
                     DDAVLIAVSPGQRVVEVVSGCRAHHRLPDRGCKLAVENMISSFKEGDLTGGLTSGLRI
                     LTEQTDS"
     gene            complement(685..912)
                     /locus_tag="VHH53_10820"
     CDS             complement(685..912)
                     /locus_tag="VHH53_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290658.1"
                     /translation="MLSPEIILILAGVPLAIMVLLGLLTLRPNFVRPPRYRPGEEWHY
                     PPVLWTANPEALHEGSPQTPGAPRGGARGQW"
     gene            complement(983..1531)
                     /locus_tag="VHH53_10825"
     CDS             complement(983..1531)
                     /locus_tag="VHH53_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX2290659.1"
                     /translation="MADKATATGALAGGRRRVLLLNATFEPLTALPLRRAIVLVVCSK
                     ADVVHGDAEGLVLHSARANVVVPSVIKLRTFVRVPYRARVPMTRAALMHRDRYRCSYC
                     GGRAETIDHVVPRSRGGEHCWENCVACCARCNHRKADRLLSELGWKLRVVPRPPRGQH
                     WRLLATVPDADPLWLPYLGEVA"
     gene            1832..2398
                     /locus_tag="VHH53_10830"
     CDS             1832..2398
                     /locus_tag="VHH53_10830"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290660.1"
                     /translation="MAHDPAAHYPAAQRLELIEHLHGRPVADPYRWLEDPADPRTIRW
                     AAEQDKLARETLDALPGRTALAGELGRLLSTGWVSVPIWRNGRAFFTRREPDGEHAVL
                     YVREAGPGGGPADRALVDVIALDPSGLTTLDSWSPSQEGDRLAYQISTGGDEESTLQV
                     LDVATGERLDGPIERCRYSPIWWLPGGQ"
BASE COUNT          417 a          835 c          836 g          396 t
ORIGIN      
        1 ccgctgtccg acgctggccc gcttccgctt cggcgccggt ggtcccgact cgtccacaga
       61 ctcgaccacc acagagacgg tcaccgtcgc cgcgagacgg atccggcgcg aacgaaactc
      121 cgcgtgggat gtcgctcgag tctccgtcac acgatcgagt atgcggccag gactttcctc
      181 gatgccagac aacattagga gtctgtttgc tccgtgagga tgcgcaggcc cgaagtcaga
      241 cctccggtga gatcgccctc cttgaatgac gaaatcatgt tttccaccgc aagcttgcac
      301 ccacgatccg gaagccggtg gtgagccctg cagccgctga ccacctctac tacccgttgg
      361 ccgggtgaga cggctatcag cactgcatcg tcggcccggc cgcccatcaa ctcgtgcagt
      421 tcctcggccc gtgcccgggt atcaggaccc aggtcgccga ggtatacacc gaagtgcagg
      481 cccacactgc ggctggcgca gaccagtgcg tcgtcgagcg ctgcacgctg cgacttggta
      541 aagggcaggg taggtagcac cggcctgact tgccgggcgg tggagatccg tccagtgccg
      601 accacgacct cgccgagcgc gcgttcctgc tggtcactgt cgtgtggggt ttggtgacgc
      661 cgcgcaggct tgccgtgctc ctcatcacca ttggccacga gcacctcccc gcggtgcacc
      721 tggtgtctgg gggctaccct cgtgcagcgc ctcggggttc gccgtccaca gcacgggcgg
      781 gtaatgccat tcctcgccgg ggcggtagcg aggaggccgg acgaaattgg gccgcagggt
      841 cagcagcccc agcagcacca taatggccag cggaacgccc gccaggatga ggatgatctc
      901 cggagacagc acaggatcac ggtagctgac cgcaacataa cctcgcctcc ccaggtgggg
      961 cggttgctga gagctgcccg cctcaggcca cctcaccgag gtagggcagc cacagcgggt
     1021 cggcgtcggg caccgtggcc agcagccgcc agtgctgccc gcgcgggggg cgcggcacca
     1081 cccgcaactt ccaccccagt tcactgagca gccggtcggc cttgcggtga ttgcagcggg
     1141 cacagcaggc cacgcagttc tcccagcaat gttcaccgcc gcggctgcgc ggcaccacgt
     1201 gatcgatggt ctccgcccgg cctccgcaat aactgcaccg ataacggtcg cgatgcatca
     1261 acgccgccct ggtcatcggt acccgcgccc ggtaaggcac tcgcacgaag gtacgtagct
     1321 tgatcaccga gggaaccaca acgttggccc ttgcggaatg cagcaccaag ccttcagcgt
     1381 caccatgcac cacatcggct tttgagcaca ccaccaggac aattgctcgg cgcaacggca
     1441 gcgcggtcag cggctcaaag gtggcgttga gcagcaacac acgtcggcgg cctccggcga
     1501 gcgcgccggt ggcggtggcc ttgtctgcca cgacaacagt ggtatcgagg agcccggttg
     1561 aggttcctga gcgcgaggcg gtccgactgg ccgcccccgc tgggtgaccc tccgggctgg
     1621 gttgtcggtc gtgcacgcga ccacctccgg caattcgcgc cactagtgca ccatgaaggc
     1681 ggcgatcgcg cacgtgtctg atgttgacac gtgtcggtga ccaccgatcg agccaaccgc
     1741 gacagcaggt gattggacgg cggggataca gtgccctccc gtggatggaa ctgaggccgc
     1801 gcctgccccg tctccggcgg gccacgaccc gatggcccac gatccggcgg cccactatcc
     1861 ggcggcacag cggttggagc tcatcgagca cctgcacggg cgaccggtgg ctgaccccta
     1921 ccgctggctg gaggacccgg ccgacccgcg caccatcagg tgggcagccg agcaggacaa
     1981 gctggcgcgc gagacgttgg acgcgttgcc cggccggacg gcgctggctg gagagctcgg
     2041 gcggttgctg tccaccggat gggtatcggt gccgatctgg cgcaatgggc gggccttttt
     2101 cacccggcgt gaaccggacg gggagcacgc cgtgctgtac gtccgggagg ccggaccggg
     2161 cggcgggccg gccgatcggg cgttggtgga cgtcatcgcc ctcgacccgt ccgggctgac
     2221 cacgctggat tcgtggagcc cgagccagga gggtgatcgg cttgcttacc agatctccac
     2281 cggtggtgac gaggaatcca cgctgcaggt gctcgatgtg gccactgggg agcggctaga
     2341 cggaccgata gagcgctgcc ggtactcacc catctggtgg ttacccgggg gtcagtagct
     2401 ctactacgtg cgacggctgc cggctgatga agtgcccgat ggggaggagc agttccatcg
     2461 ccgggtctgg cgacaccgtg tcgg
//
LOCUS       DATADN010000835         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1465494, whole genome shotgun sequence.
ACCESSION   DATADN010000835 DATADN010000000
VERSION     DATADN010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465494"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1516
                     /locus_tag="VHH53_10835"
     CDS             <1..>1516
                     /locus_tag="VHH53_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2290661.1"
                     /translation="GVKAGSAALQSLPEELTEVPNAQTTYVYANDGRTLLTTFYEEHR
                     KPTKLKDMSPYVTQAIVASEDTRFYEHNGVDPKGVARAFVANQKAGGVSQGASTLTMQ
                     YVRMALRDGAKTPKDAIEATEQTTARKLREMRLAIELEKRASKPEILERYLNSAYFGH
                     RAYGIFAAAQVFFSKTPAKLNLTEAALLAGLVKAPSAYDPALEGNQRAATDRRNYVID
                     QMLRMGVITAAQAGTAKKTKIKLRLSTPPNDCVSVAEKHNDWGFFCGYFRNWWLEQPA
                     FGATAQEREQKLRRGGYKVVTTLDPKIQAYALEHVLDKEDRDSKFAHGEVVIEPGTGR
                     IKAMAVNRTFSLNQKDNGKHTDRAKRRDGLKGNYPNTVNPLLGGGDMAGYQAGSTFKI
                     FTMLAALDDGRKLSTSFNAPPRLVTRYITAPGPATCGIRWCPKNSSASMSGRQTMWSG
                     FGKSVNTYFVWLEQKVGAEKAVKMAERLGLRWRTEIDRTLAQPDRATGWGAFTLG"
BASE COUNT          293 a          503 c          530 g          190 t
ORIGIN      
        1 acggggtcaa ggccgggtcg gccgccctgc agagcctgcc ggaagagctc accgaggtgc
       61 ccaacgcgca aacgacgtac gtctacgcca acgacggccg cacgctgctc accacgttct
      121 acgaggagca ccggaagccg accaagctga aggacatgtc gccgtacgtg acgcaggcga
      181 tcgtggcttc cgaggacacc cggttctacg agcacaacgg cgtggatccc aagggcgtgg
      241 cccgggcgtt cgtggccaac cagaaggccg gcggcgtctc gcagggcgcg tccacgttga
      301 ccatgcagta cgtccggatg gcgctgcggg acggggcgaa gacgccgaag gacgcgatcg
      361 aggccaccga gcagaccacc gcccgcaagc tgcgcgagat gcggctggcc atcgagctgg
      421 agaagcgcgc ctcgaagccg gagatcctcg agcgctacct gaactcggcg tacttcgggc
      481 accgcgcgta cgggatcttc gccgccgccc aggtgttctt ctccaagacc ccggccaagc
      541 tcaacctgac cgaggcggcg ctgctggccg ggctggtcaa ggcgccgtcg gcgtacgacc
      601 cggctctgga gggcaaccag cgcgcggcca ccgaccggcg caactacgtg atcgaccaga
      661 tgctgcggat gggtgtgatc accgcagccc aggccggcac ggcgaagaag acgaagatca
      721 aactgcggtt gagtacgccg ccgaacgact gcgtgtcggt cgccgagaag cacaacgact
      781 ggggcttctt ctgcggatac ttccgcaact ggtggctgga gcagccggcg ttcggcgcca
      841 ccgcccagga gcgggagcag aagctgcgcc gcggcggcta caaggtggtc accacgctgg
      901 acccgaagat ccaggcgtac gccctggagc acgtgctgga caaggaggac cgggacagca
      961 agttcgcgca cggtgaagtg gtgatcgagc cgggcaccgg ccggatcaag gcgatggcgg
     1021 tgaaccgtac gttctcgctg aaccagaagg acaacggcaa gcacaccgac cgggccaagc
     1081 gccgcgacgg cctcaagggc aactacccga acacggtgaa cccgctgctc ggcggcggtg
     1141 acatggccgg gtaccaggcc gggtccacgt tcaagatctt cacgatgctg gccgccctgg
     1201 acgacgggcg gaagctcagc acctcgttca acgcgccacc gcggctggtc acccgctaca
     1261 tcaccgcacc cggcccggcc acctgcggca tccgctggtg cccgaagaac tccagcgcct
     1321 cgatgagcgg ccggcagacc atgtggagcg ggttcggcaa gtcggtgaac acgtacttcg
     1381 tctggctcga gcagaaggtc ggcgcggaga aggcggtgaa gatggcggaa cggctcggcc
     1441 tgcggtggcg taccgagatc gaccggacgc tggcccagcc cgaccgggcc accggctggg
     1501 gcgcgttcac gctggg
//
LOCUS       DATADN010000836         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_87369, whole genome shotgun sequence.
ACCESSION   DATADN010000836 DATADN010000000
VERSION     DATADN010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87369"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VHH53_10840"
     CDS             complement(<1..839)
                     /locus_tag="VHH53_10840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2290662.1"
                     /translation="MRTNRPFRLLWTARTVSFLGDALSLVALMLHVAETTGQALAVAV
                     LLLVGDFAPALLGPLTGTISDRFDLKRVMVGCELAQAGLMAVIALWLPPLPLLLALVA
                     LRAIAGQIFQPASRAAVPGVVRDRDLESANAAIGIGTNGGEALGPLLAALLLPVLDAR
                     GVLLLDVATFLASAALLTFLPSPKGDFSTGTARGTFLADTKAGLRYIWATPMVRTIGL
                     GFFPIVAFNGIDDVALVFLATDTLASDGMGGGDAAAGLLLAAVGIGLFIGYALLARPH
                     RLA"
     gene            complement(968..>1559)
                     /locus_tag="VHH53_10845"
     CDS             complement(968..>1559)
                     /locus_tag="VHH53_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEX2290663.1"
                     /translation="ALRQQPTWNATTLRSGSRERAVHLRSSERDPHSDPGISHGGMVF
                     LVGLAIPALVVWIRARKLHDGSRGFTTSTLIIVCGAFILDAALTPSPKPSATIAEAVP
                     AAPVEQAAVPQPLVVVPPASLVVPAPQPLVQTRPPAAATPRVVPAPVPQTPAGSAICY
                     KNCAVTRAAGAAPIQKGEPGYRSALDGDRDGTACDT"
BASE COUNT          296 a          514 c          545 g          204 t
ORIGIN      
        1 atcgcgagcc ggtgtggccg ggcgagcagc gcgtatccga tgaacagccc gatgccgacc
       61 gcggccagca gcagcccagc cgcagcgtcc ccgccgccca taccgtcgga agccagtgtg
      121 tcggtggcca agaacaccaa cgcgacgtcg tcgatgccgt tgaacgcgac gatcgggaag
      181 aagccaaggc cgatcgtccg gaccatcgga gttgcccaga tgtagcgcag cccggccttg
      241 gtgtcggcca ggaaggttcc ccgcgcggtg ccagtggaga agtctccctt cggggacggc
      301 aggaaggtga gcagcgccgc cgaggcgaga aaggtggcga cgtcgaggag cagaaccccc
      361 cgggcgtcca ggaccggcaa cagcagcgcg gcgagcagcg ggccgagtgc ttcaccgccg
      421 ttggtcccga tcccaatcgc agcgttggca ctctccaggt cccgatcccg caccacaccg
      481 ggaaccgcgg cgcgtgacgc cggctggaag atctgcccgg cgatcgcgcg cagtgcgacc
      541 agagcgagca gcagcggcaa aggtggcagc cacagcgcga tcacggccat caggcccgct
      601 tgggccagtt cacagccgac catgacgcgt ttgagatcga agcggtcgct gatcgtgccg
      661 gtcagcggcc cgagcagcgc aggagcgaaa tcaccgacca ggagcagtac cgccaccgcc
      721 agcgcttggc cggtggtctc ggcgacgtga agcatcagcg ccaccaggct gagcgcgtcg
      781 cccaggaacg acacggtgcg cgcggtccac agcaaccgaa acggccggtt cgtgcgcacc
      841 aggcgaagcg agccgcccct gctcgcctcg ttagccggca cgatcgacga gtctgacgcc
      901 gatcaccggt tgaggcagcc gaatatctct tggcgcagcc gtggttattc gggaaatagg
      961 cgccgtctta cgtgtcgcac gccgtaccgt cgcggtcacc gtcgagtgcg gagcgatatc
     1021 cgggctcgcc cttctgaatg ggggctgcgc cggcagcacg cgtcaccgcg cagttcttgt
     1081 agcaaatggc gctgccggct ggcgtttgcg gcacgggcgc cggcaccacc cgaggggtgg
     1141 cagcagcagg agggcgggtc tgcacgagag gctgcggggc cggcacgacc agggatgcag
     1201 gaggaaccac cacgaggggc tgcggcactg cggcctgctc gaccggcgca gcgggcaccg
     1261 cctcggcgat cgtcgcggaa ggcttcggac tcggagtcag ggcggcgtcc agaatgaacg
     1321 ctccacacac aatgatcaga gtgcttgtcg tgaacccacg gctgccgtcg tgcagcttcc
     1381 gcgcacgaat ccagacgacg agcgcaggga tagcgagccc caccaagaac accatcccac
     1441 catgcgagat cccgggatca gagtgaggat cgcgctcaga agaacgaagg tgaacggcac
     1501 gttcccgact gccgctgcgg agcgttgtgg cattccaggt cggttgctgt cggagggct
//
LOCUS       DATADN010000837         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_244907, whole genome shotgun sequence.
ACCESSION   DATADN010000837 DATADN010000000
VERSION     DATADN010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244907"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VHH53_10850"
     CDS             complement(<1..334)
                     /locus_tag="VHH53_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2290664.1"
                     /translation="MPATTMGVPEESLRGFEAAWDEFFAAIRRARGRAAREHGDELTL
                     SQYNLLGALGEHSELPVGEVALAAGVAAPTATRMLDHLQRAGIVRRAHSTRDRRVVTV
                     ALTTQGHQL"
     gene            471..1370
                     /locus_tag="VHH53_10855"
     CDS             471..1370
                     /locus_tag="VHH53_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX2290665.1"
                     /translation="MRVLLTGWPSFLNGEATAGDVLSMDQVRRGLVSSGVPCEVAWSQ
                     VFRPGSLGLDDADPQRYTHLVFVCGPAHGEQVQWLHQRFSRCRRIAVGVTVCDPSDPA
                     VTGFDVVLARDTPQGQGRRDLSATARTDHVPVVGVVLAPGQREYGKRRRHDEVHQRIT
                     TWLLKQDCARVPVDTRLDPRQWRNPSTPDQLDSVLCRLDAVLTTRLHGLVLALRQGVP
                     VLAVDPVDGGGKVGAQAAAWAWPALLTAGEVIKDVDCLRPWWNWCLSGEGRMLAAERA
                     TAAFRDGEQLIADLLGILRVEFA"
     gene            1384..1560
                     /locus_tag="VHH53_10860"
     CDS             1384..1560
                     /locus_tag="VHH53_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290666.1"
                     /translation="MSSAELPDVDAIEQQTLIDDVEDREDDLTQLPTEVDPADAVEQH
                     RDLGGDDVDDYPWG"
BASE COUNT          263 a          510 c          574 g          264 t
ORIGIN      
        1 gcagctggtg gccctgcgtg gtgagtgcca ccgtcaccac gcgccggtcc ctggtggagt
       61 gcgcgcgccg cacgatgccg gcgcgctgca ggtggtcgag catccgcgtt gcggtcggcg
      121 cggccacgcc cgcagccagc gcgacttccc caacaggcag ctccgagtgc tcaccgagtg
      181 cccccagcag gttgtactgg gacagcgtga gctcgtcacc gtgctcgcgc gcggcacgtc
      241 cgcgggcacg gcggatcgct gcgaagaact catcccacgc agcctcgaag ccccgcagcg
      301 attcctcagg aactcccatg gtcgtcgcag gcatgacttg aagctagcaa aatattagct
      361 agctatctat cttctcatcg gccaaccggc gtaggccact tgcgcatgaa ccgtgatcgg
      421 tcgggtttcc ggcactgcag cgcgggtagc tcgatcgcga tggggggcgc atgcgagtgt
      481 tgctgaccgg gtggccgagc ttcctgaacg gtgaggcgac cgcgggtgac gtgttgagca
      541 tggaccaggt acgtcgtgga ctggtgagct ccggcgtgcc gtgcgaggtg gcgtggagcc
      601 aggtgttccg cccgggcagt ctcggcttgg acgatgccga tccgcagcgc tacacgcatc
      661 ttgtgttcgt ctgcggaccg gcgcacggcg agcaggtgca atggctgcac cagcgcttca
      721 gccggtgtcg ccggatcgca gtgggtgtca ccgtgtgcga cccgtcggat cccgcggtca
      781 ccggattcga cgtggtgctc gcccgcgaca ccccgcaagg gcagggccgc cgggacctct
      841 cggccacggc gcgcactgac cacgtgccag tcgttggcgt ggtgcttgca ccaggtcaac
      901 gggagtacgg caagcgacga cggcacgacg aggtgcacca acggatcacg acgtggttgc
      961 tgaaacagga ctgtgctcgg gtgccggtgg acacccggct cgacccgcgg caatggcgca
     1021 accccagcac gccagaccag ctcgactcgg tgctgtgccg gctggacgcg gtgctcacca
     1081 ctcggctgca cggcctcgtg ctggcgctgc gccagggagt gcccgtactg gccgtggatc
     1141 cggtggatgg cgggggcaaa gtcggcgcgc aggccgctgc ctgggcctgg cccgcgcttc
     1201 ttaccgccgg cgaggtgata aaggatgtgg attgcctgcg gccgtggtgg aactggtgcc
     1261 tgtcggggga aggccggatg ctggcagccg agcgtgccac agccgccttt cgtgacggtg
     1321 agcaactgat cgccgatctg ttgggaatac tgcgagtcga gtttgcttga gaacaggagc
     1381 agcatgagta gtgccgaact gcccgatgtc gacgcaatcg agcagcagac attgatcgac
     1441 gatgtcgagg accgcgagga cgacctcacc caactaccta ccgaggtcga tcccgccgac
     1501 gcggtcgaac agcaccgcga cctgggcggg gacgacgtcg atgactaccc atggggctga
     1561 gcgggctacc ggtggggttc gacggcgctg atgtcagcca gcgccgaggt c
//
LOCUS       DATADN010000838         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_706878, whole genome shotgun sequence.
ACCESSION   DATADN010000838 DATADN010000000
VERSION     DATADN010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706878"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1048)
                     /locus_tag="VHH53_10865"
     CDS             complement(149..1048)
                     /locus_tag="VHH53_10865"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX2290667.1"
                     /translation="MSLGGEVLLVLHTGHPENLRIVEKVANRLAAARIGLRVLAEEAT
                     HLDPALCGQEMPTGPDAAAGADLVMVLGGDGTLLRAAELARPCGVPLLGVNLGRVGFL
                     AEAELDSLDEAIHAIVAGDYLVDERMTVEVIATLNGSTLARTWALNEASVEKSRRERI
                     LDVVLEVDRRPVSGFGCDGVLCATPTGSTAYAFSAGGPLVWPDVDALVVVPSNAHALF
                     ARPLVVSPDSVVAVETDPDGNAAVLCCDGRRTFELPAGTRVEVVRGATPIRLVRLHDV
                     PFTDRLVRKFELPVQGWRGPKSQ"
     gene            complement(1059..1874)
                     /locus_tag="VHH53_10870"
     CDS             complement(1059..1874)
                     /locus_tag="VHH53_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX2290668.1"
                     /translation="MVRRARLDAELVRRGLARSRDHAAELVAQRRVRIHGVTATKPAT
                     GVDPDTPLLVAALGNEPEWVSRGARKLLGALDALTGGGPTIAGRRCLDAGASTGGFTD
                     VLLHRDAREVVAVDVGYGQLAWSLRTDPRVWVHDRTNVRTLQAEDIGGTVDLTVADVS
                     FISLRLVLPALAGCTTPDGDLLPMVKPQFEVGRENLGAGGVVRDPVLRAQAMLDVAAV
                     AGNLGLCLVDATASPLPGPSGNVEYFLWFRRGPADSRRRAEELVHTAVQEGPP"
     gene            complement(1875..2030)
                     /locus_tag="VHH53_10875"
     CDS             complement(1875..2030)
                     /locus_tag="VHH53_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290669.1"
                     /translation="MTSQPPPCDTGIADALAGLHGLDELATADHVARFNAVQDSLTAA
                     LSNIDEV"
BASE COUNT          381 a          756 c          729 g          329 t
ORIGIN      
        1 ctccggtctc accagtcacc acggtcagac cggggtgcag gtccagggtg gcctcgtcga
       61 tcacaccgag gccctcgatg cgcatctcgg tgagcacggg ccgcacccta gcgtgtcaaa
      121 cactctgggg cggtcaacgt aggggcggtc actgtgattt ggggccccgc caaccctgca
      181 ccggcaactc gaacttgcgc accaagcggt cggtgaacgg aacatcgtgt aggcgcacca
      241 gccggatcgg cgtggcgcca cggacaacct cgacccgagt gcccgccggc agctcgaagg
      301 tgcgccgtcc gtcgcagcac agcaccgcag cattgccatc cggatcggtc tcgacggcca
      361 ccaccgagtc cggtgacacc acaagcgggc gagcaaacag cgcatgggca ttgctgggca
      421 cgacgacgag cgcgtcgacg tccggccaca ccagcggtcc accggcggag aaggcatagg
      481 cggtggagcc ggtgggggtg gcgcagagca ctccgtcgca gccgaacccg gagaccggcc
      541 tgcggtccac ctcgagcacc acgtcgagga tccgttcccg gcggctcttc tcgacgctgg
      601 cctcgttgag tgcccaggtt cgagccagcg tggaaccgtt tagggtggcg atgacctcga
      661 cggtcatccg ctcgtcgacg aggtagtcgc cggcgacgat ggcgtgaatc gcctcatcga
      721 gggagtccag ctcggcttcg gcgaggaaac cgaccctgcc caagttcacc ccgagcagcg
      781 gaacgccgca cggtcgggcc agttcggcag cccgcagcaa ggtgccgtcg ccaccgagca
      841 ccatgacgag atccgcgcct gcggcggcgt ccggaccggt gggcatctcc tggccgcaca
      901 gcgcgggatc gagatgggtg gcttcctcgg cgagcacccg cagcccaatt cgggcggcgg
      961 ccagccggtt tgcgaccttt tctacgatac gcaagttctc gggatgcccg gtgtgcaata
     1021 ccagaaggac ctcgcctccc agactcattg tgccagcttc atggcggacc ctcctgcaca
     1081 gcggtatgca ccaattcttc ggcgcgacgc cgcgaatcgg ccggaccgcg gcgaaaccat
     1141 aagaagtact caacgttgcc cgacggccct ggcagtgggc tcgccgtggc gtcgaccagg
     1201 cagagcccga gattaccggc gaccgctgcc acgtccagca tggcctgcgc gcgcagcacc
     1261 gggtcacgga ccacgccgcc ggcccccagg ttctcgcgac ccacctcgaa ctggggtttc
     1321 accatcggaa gcaggtcgcc gtcgggtgtg gtacagccgg ccagcgcagg tagcaccagg
     1381 cgcagtgaga tgaaggacac gtcagccacg gtcagatcaa cggtgccacc gatgtcctcg
     1441 gcctgcagcg tccgtacgtt cgttcggtca tgcacccaga cccgtgggtc agtacgcaat
     1501 gaccaggcga gctgcccgta gccgacgtcg acggcaacca cttcacgagc gtcgcgatgc
     1561 agcaacacgt cggtgaagcc gccggtggac gcgccggcat ccaggcaacg ccggccagcg
     1621 atggtaggac ctcccccggt gagcgcatct agtgcaccga gcagctttcg ggcgccccgc
     1681 gacacccact caggttcgtt tcccaacgct gcgaccagca acggggtgtc gggatcgact
     1741 ccggtggccg gcttggtagc tgtcactccg tggattcgga ccctccgctg tgccacaagt
     1801 tcagcggcgt gatcgcgaga tcgggccagg ccgcgccgta ccagttctgc gtcgagccgg
     1861 gccctgcgga ccacctagac ctcgtcgatg ttggacaggg ctgcggtgag cgagtcctgt
     1921 acggcgttga accgggcaac gtggtcagcg gtcgcaagct catcgaggcc gtgcaggccg
     1981 gcgagcgcgt cggcgatgcc ggtatcgcag gggggtggct gggatgtcat ggagctttct
     2041 cctcaacctg tttccggggc ctatgtccgg aacgctcggg tcccgccacg ttaccgagcg
     2101 gcatcgcgca atcccacggc gaccagtgct gcgtcggcgg cagcgtcaca ggcatgaacg
     2161 tccaccggcc cggcgtgctc agcccaccag gcggc
//
LOCUS       DATADN010000839         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1466800, whole genome shotgun sequence.
ACCESSION   DATADN010000839 DATADN010000000
VERSION     DATADN010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466800"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1595
                     /locus_tag="VHH53_10880"
     CDS             42..1595
                     /locus_tag="VHH53_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2290670.1"
                     /translation="MQSDPDTCDVLIVGAGPAGLTVATMLARHGVDVLVVERHDGTWP
                     FPKATGVSTRTMELLRSWGLEQQVRVGAMRVRPGLVVSNTLADPMQKADLPFGYPTDE
                     AALAVSPATPCYCPQDHLEPVLLEHLRDHGGRVQFGTEMINFTAGGADVLAELRDRAS
                     GRAERVQAQYLIGADGPRSTVRAGLGIDVDDLGTIGDFIAVTFRADLTRRLPRPPSVL
                     NAVEVAGAAGLFVPASTDDRWIYGREWHPERGESIADWTPQRCTELLRAGTGLPDLQP
                     EILAVMPFVMGGHVATTFRAGRTFLVGDAVHRTTPVGGIGMNTAIHGAHNLGWKLAWV
                     LRGWAGEALLDSYEAERRPIGTDNVLRSLRLSPQRAGEGLAWDIDVRYTSAVVEARFG
                     AGERAPHAWLRCDGARMSTLDLFDGRLTILTGPRGASWRRAATELAADGLPIVALSAD
                     RDLHDEHGAFARHYRLGDAGAALVRPDGYLAWRRLAPCGDARTSLRSAVALALGRAAA
                     APATLRQAG"
BASE COUNT          237 a          604 c          590 g          277 t
ORIGIN      
        1 ggaccacggg cttatcgagc cacccggcga aggagcttct catgcaatcc gaccccgaca
       61 cctgcgatgt cctgatcgtc ggcgccgggc cggccggtct gaccgtcgcg accatgctcg
      121 cccggcacgg cgtggacgtc ttggtggtcg agcggcacga cggcacgtgg ccgttcccga
      181 aggccaccgg tgtcagcact cgcacgatgg agctgctccg cagctggggt cttgagcagc
      241 aggtccgcgt gggagccatg cgggtgcggc cgggtctggt ggtgtccaac accttggctg
      301 atccgatgca gaaggccgac ctgccgttcg gctacccgac cgacgaggcc gctctggccg
      361 tcagcccggc cacgccatgt tattgcccgc aggatcacct cgagcccgtg ttactagagc
      421 acctgcgcga ccacggcggc cgcgtccagt tcggcactga aatgatcaat ttcacggccg
      481 gcggcgctga tgtgctcgcc gagctgcgcg accgcgcctc agggcgcgct gagcgggtcc
      541 aggcccagta cctgatcggc gcggacggtc cgcgcagcac ggtgcgtgcc ggtctaggca
      601 tcgatgtcga cgacctcggg acaatcggtg acttcatcgc ggtgaccttc cgcgccgatc
      661 tcacccgccg gctgccccgt cccccgtccg tgctcaacgc cgtcgaggtc gcaggcgccg
      721 cgggactgtt cgttccggcc agcaccgatg accggtggat ctacggccgc gagtggcacc
      781 ccgagcgcgg cgagtcgatc gcggactgga cgccccagcg gtgcaccgag ctgctgcggg
      841 ccggcaccgg cctgccggac ctgcagcccg agatcctcgc cgtgatgccc ttcgtcatgg
      901 gtggtcacgt cgccaccacg ttccgcgccg gtcggacgtt cctcgtcggt gacgccgtgc
      961 accgcaccac gccggttggt ggcatcggga tgaacaccgc gatccatggc gcgcacaacc
     1021 tcggctggaa gctcgcgtgg gtgcttcgcg gttgggccgg cgaggcactg ctcgacagct
     1081 acgaggccga acgtcgaccg atcggaaccg acaacgtgct gcgctcgctg cgcctcagcc
     1141 cccagcgcgc gggagagggt ctggcttggg acatcgacgt gcgctacacc tcggcggtag
     1201 tcgaagcccg ctttggcgcg ggggaacggg caccgcatgc gtggctgcgc tgcgacggtg
     1261 cccggatgtc gacgctcgac ctgttcgacg gccgtctcac aatcctcacc ggcccgcggg
     1321 gtgcgtcgtg gcgccgggcg gccactgagc ttgctgctga tggcctgccg atcgtggcgc
     1381 tgtccgcgga ccgcgaccta catgatgagc acggcgcatt cgcccgtcac taccgcctgg
     1441 gcgacgcggg tgcggccctc gtccgccccg atgggtatct cgcatggcgg cgtctcgcgc
     1501 catgcggcga cgcacggacg tcactgcggt cggctgttgc gcttgctctg ggcagggccg
     1561 ccgcggcacc ggccaccttg cgccaggccg ggtagccagc ttccaccgca aatcccgccg
     1621 aggacctgat cgacgcggtg actcgctgga tcgtgttatc ggcagctagg ttgccgcaat
     1681 agcggtgtgt ccaggagcag caccttcg
//
LOCUS       DATADN010000840         1952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_707076, whole genome shotgun sequence.
ACCESSION   DATADN010000840 DATADN010000000
VERSION     DATADN010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707076"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..316)
                     /locus_tag="VHH53_10885"
     CDS             complement(182..316)
                     /locus_tag="VHH53_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290671.1"
                     /translation="MASWIEARLLALPIPLAIFGPRDEGELEIVSGIAGASHSFAAHG
                     "
     gene            complement(349..1635)
                     /locus_tag="VHH53_10890"
     CDS             complement(349..1635)
                     /locus_tag="VHH53_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2290672.1"
                     /translation="MRTRIVALAVLAAVLAIGLFGVPLAAGALKYALSEERTTLLRMA
                     DAAAVAVTADVLRGNMPTDLPTPQDETHLAVYVDRGLRILGTGPQGGDAIVDRALHGD
                     IYTGDLAGDLVVAVPVTHDSDVIGAVRAASPRSAVYRQVMLVWLGMLGLAALAIGTVW
                     LVARRQARRLAGPLEDLSDAARRLGDGDFSVRTESVDIPEIDAVGTALNSTAGRLDDM
                     LARERAFSADASHQLRTPLTGLRLRLEAALDRPGQDLRHAITAGIDDADRLEQTIEEL
                     LALARDTRSSSSAPLDLSGLLDEIERGWHDRLAAQDRKLQVAVDSAAPVSLASTAAVR
                     QVLTVLLDNAATHGSGTVSVAVRNAADALAIDVSDEGIGITAPEPELFTRRSRLADGH
                     GIGLALARSLAEAEGGRLRLTRPAPPTFTLLVPAQG"
BASE COUNT          336 a          669 c          643 g          304 t
ORIGIN      
        1 gcgcctggct ggtcgcggtg ggagtcgtcg gagtcagcga gcacagtgct cggtactggc
       61 tgtcccacca gcgcgaggta cccaaggacg aggccgtggc gctgaccgcg tcactggcct
      121 ggaaaggcct cgccggcttc ccgctgcaac ataacgttgc aacgtaacga ggccaggcga
      181 ttcaaccgtg cgcggcgaag gagtgactcg cacccgcgat cccggacacg atctccagct
      241 caccctcatc gcgcggtccg aaaatcgcca acgggatcgg cagtgccagc agccgtgcct
      301 cgatccagct cgccaactcg gacgcggagg acctgcccag gcgctcgttt acccctgggc
      361 gggcaccagc aacgtgaagg tagggggagc gggccgggtc agccggagcc tgccgccttc
      421 ggcctcggcc aggctgcggg cgagcgcgag tccgatcccg tgcccgtcgg ccaggcgcga
      481 tcgccgggtg aacagctccg gttcaggcgc cgtgatgccg atgccctcat cggagacatc
      541 gatggccagc gcgtcagcgg cgttacgcac cgccacagat accgtgcctg agccgtgggt
      601 tgccgcattg tccagcagca ctgtgagcac ctgccgcaca gccgcagtgg aggcaagtga
      661 cactggagcc gcggagtcca ccgccacctg cagcttgcgg tcctgggcgg ccagccggtc
      721 atgccagcca cgctcgatct cgtcaagcaa gccggagagg tccagcggtg cagagctgga
      781 actacgggtg tccctggcca gggcaagcag ctcctcgatg gtctgctcca gccggtcggc
      841 atcgtcaatc ccggcggtga tggcgtgccg gagatcctgc cctggcctat ccagcgccgc
      901 ctcgagccgg agccgcaatc cggtcagcgg ggtacggagc tggtgggagg catcggccga
      961 aaatgcacgt tctctggcca acatgtcgtc cagacggccg gcggtgctgt tcagcgccgt
     1021 gcccacggcg tcgatctcgg gaatgtccac tgactcggtt cggacgctga agtcaccatc
     1081 cccgaggcgg cgcgccgcat cggaaaggtc ctcgagcgga cccgccaacc gacgggcctg
     1141 gcgacgggcg accaaccaca ccgtccctat ggccagggcg gccagcccca gcatgcccag
     1201 ccacaccagc atcacctgcc ggtacacggc actgcgcggg ctggcagcac gaacggcgcc
     1261 gatcacatcg ctgtcatggg tcaccggcac ggcgacgacc agatcaccgg caaggtcccc
     1321 ggtgtagatg tcaccgtgca gggcacgatc gacaatggcg tccccgccct gtggcccggt
     1381 accaagaatg cgcaggccgc ggtccacgta gaccgccagg tgggtctcgt cctgcggtgt
     1441 ggggagatcg gttggcatgt tgccgcggag cacgtctgcc gtgacggcca ccgcggcggc
     1501 gtcggccatt cgcagcagcg ttgtccgttc ttccgacagc gcatacttca gtgcgccagc
     1561 ggccagcggt acgccgaaca gtccgatggc aagcactgcg gcgagtacgg cgagggcgac
     1621 aatccgagtc cgcacgacgc ccctcccctg tcacccgact gctcgatcaa cacagtgtct
     1681 cttaccagct ctcataccag cacagcatgc tcggggcgag atcgaccccg ttctcctgcc
     1741 ggattttgcc gacagctaac cgatctcggg ccagcggata accggggtgt ctgctcatct
     1801 tgatcccctg gaccgaacag ctcagatcac cgcggtaggg tcgttgcaat ggcaattcgg
     1861 ctgctgcatg gtgggggaca gcggtgaccg tccgatatgt ggccgcccgc tacgtagtcg
     1921 tcccggtcat cgggttggta tcgatcgtca gc
//
LOCUS       DATADN010000841         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_707224, whole genome shotgun sequence.
ACCESSION   DATADN010000841 DATADN010000000
VERSION     DATADN010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707224"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VHH53_10895"
     CDS             <1..612
                     /locus_tag="VHH53_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX2290673.1"
                     /translation="GLFTRTQPGLDRPDLMMHYGSVPFDLNTVRWGYPTTSNGFCLTP
                     NVTRGRSRGTVRLRTRDFRDRAKVDPRYFTDPEGHDISVMLAGIKLARRIAAQPALLG
                     WVGRELAPGAGAVSDDELIDYIRKTHNTVYHLAGTARMGAADDPAAVVDPRLRVKGVT
                     GLRVADGSIMPFLPSVNPNITTMMIGEKASDLILEDATRHSAG"
     gene            complement(601..1563)
                     /locus_tag="VHH53_10900"
     CDS             complement(601..1563)
                     /locus_tag="VHH53_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEX2290674.1"
                     /translation="MDAPLLLLDAASLYFRAYHGVPESITAPDGTPVNAVRGFTDMVA
                     RLIESRRPSRLVACLDLDWRPAFRVAAIPSYKAHRVAEETGPGATDVEQVPDTLSPQV
                     PLILELLATAGLATAGAEGYEADDVIGALADAERRDPVEVVSGDRDLFQVVRTEPTPV
                     RVVYVGRGLAKAEVIGPIELARRYGLPETGAGPGYADLAALRGDPSDGLPGVPGIGEK
                     TAATLISRFGSVEGLLVALDDPRSALAPGMRAKLRASRHYLELAPAVVRVATDAPVRQ
                     DRDDTVPVVPADADALTALQRRWGLGAAVDRLVAALSGPTHSSC"
     gene            1632..>1858
                     /locus_tag="VHH53_10905"
     CDS             1632..>1858
                     /locus_tag="VHH53_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX2290675.1"
                     /translation="MPAVLDTLTSRLTRAQQATQVAGLDALLITPSSDLRYLVGMAGE
                     SHERLTCLVLPADGDPALVVPALERPGFDATP"
BASE COUNT          299 a          675 c          612 g          272 t
ORIGIN      
        1 ggcctgttca cccggacgca accgggactc gatcggcctg acctgatgat gcactacggc
       61 tcggtgccgt tcgacctgaa taccgtgaga tggggctacc cgaccacgtc gaacggtttc
      121 tgcttgacac cgaacgtcac ccgcggccgg tcccgcggaa ccgtccggct ccggacacgt
      181 gatttccggg accgtgccaa ggtggacccg cgctacttca ccgaccctga aggccatgac
      241 atttccgtga tgctcgccgg gatcaagctt gcccgccgga ttgccgcgca gccggcgttg
      301 ctgggctggg tcggacgcga gctggcaccc ggtgctggcg cggtcagcga cgacgagctc
      361 atcgactaca tccgcaagac ccacaacacc gtctaccacc tcgcgggcac ggccaggatg
      421 ggcgcggcag acgacccggc ggccgtggtc gatccccggc tacgggtcaa gggggtcacc
      481 gggctgcgag tcgcggacgg ctccatcatg ccgttcctcc catccgtcaa ccccaacatc
      541 accaccatga tgatcggcga aaaggccagc gacctcatcc tcgaggacgc gacgaggcat
      601 tcagcaggat gaatgggttg ggccggaaag ggcggcgacg agccggtcca ctgctgcgcc
      661 aaggccccat ctccgctgca gggcggtgag cgcgtcggcg tcggccggca ccacgggcac
      721 cgtatcgtcg cgatcctggc gcaccggggc atcggtagcc acccgtacca ccgcgggcgc
      781 cagctccagg tagtggcggg acgcccgaag tttggcccgc atcccggggg cgagtgctga
      841 tcgcggatcg tccagcgcca cgagcagccc ctctaccgag ccaaaccggc tgattaacgt
      901 ggccgcggtc ttctcaccga ttcccggcac cccgggcaac ccgtcggacg ggtcgccgcg
      961 caacgcggcc agatccgcgt agccgggtcc ggctccggtt tcgggcagcc cgtagcgtct
     1021 ggccagttcg atggggccga tcacctccgc tttggccaac cctcgcccga cgtagaccac
     1081 ccgcaccgga gtcggctcgg tccgcacgac ctgaaacagg tcccgatcgc cgctgaccac
     1141 ctcaaccgga tcgcggcgtt cggcgtcagc caacgcgccg atcacgtcgt cggcctcgta
     1201 gccctcggcg cccgcggtgg ccagtccggc ggtggccagc agctccagga tcagcggtac
     1261 ctgcgggctc agggtgtccg gcacctgctc gacgtcggta gcgccgggtc cggtctcctc
     1321 ggcgacccgg tgcgccttgt aggaggggat cgcggcaacc cggaaggccg gccgccagtc
     1381 caggtccagg caggcgacca gccgggacgg gcgacgggac tcgatcagcc gggcgaccat
     1441 gtccgtgaaa ccgcgcactg cgttcaccgg ggtaccgtcc ggggcggtga tcgactccgg
     1501 gacaccgtgg taggcccgga agtacaggct cgcggcatcg agcagcaaca gaggtgcatc
     1561 catgagagca gagcctgcca ggctggccgc cttgaacact cgacaagacc ctgagcccct
     1621 aagctttgag tgtgcccgct gtactcgaca cgctcacctc ccgcctgacc cgcgcccagc
     1681 aggccaccca ggtcgccggc ctggatgctc tgctgatcac gcccagctct gacctgcgct
     1741 acctggtggg gatggccggc gagtcccatg agcggctgac ctgcctggtg ttgcccgccg
     1801 acggtgaccc ggcgctggtc gtcccagcgc tggaacgccc cgggttcgac gccacccc
//
LOCUS       DATADN010000842         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_88772, whole genome shotgun sequence.
ACCESSION   DATADN010000842 DATADN010000000
VERSION     DATADN010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88772"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(485..1045)
                     /locus_tag="VHH53_10910"
     CDS             complement(485..1045)
                     /locus_tag="VHH53_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290676.1"
                     /translation="MVLLLVAVGLLIAALGTGQLALAWVSVALSAVVAAMIVMRWRRR
                     RAEPGAEPVVLLDDEPADALPVDPASDADPSEPSPQPAPVTAAEHAGEEIEAGEQDEP
                     GEPGEEDTDAADLLVVYELNDEVLVVDEHPRYHLTRCTWPDHEQGERLPVREARELGF
                     TPCDRCRPDAALARRHRAASAATGRS"
     gene            complement(1136..1930)
                     /locus_tag="VHH53_10915"
     CDS             complement(1136..1930)
                     /locus_tag="VHH53_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX2290677.1"
                     /translation="MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVEAELARLIE
                     ENNDLRNQVEELDRQLSAAPIDTGRNLRPLEPPRPVMAPVPPPMVEQTAPGGDHHVQA
                     AKVLGLAQEMADRLTGEAKAEADGMLGEARTKSEQLLSDARAKADGMVNEARTRAETM
                     LSDARTRAETLERQSREKASSLERDAARKHTEILSSINQEKTTLEKKIDELRTFEREY
                     RTRLKTYLESQLRELDGRGSAAPADTVRSQQGFVTSGFGARAEAGS"
     gene            complement(1996..2283)
                     /locus_tag="VHH53_10920"
     CDS             complement(1996..2283)
                     /locus_tag="VHH53_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX2290678.1"
                     /translation="MDVVRLVVYYLLFFFWLLLTARIVVELVRAFARSWQPVGGVAIA
                     LESVYTMTDPPVRALRRVVKPIRIGGVGLDLSIMVLLLVVFILMRVIDPRT"
BASE COUNT          378 a          795 c          719 g          410 t
ORIGIN      
        1 cgtcatagaa gacgaattcg cctgcgccga tgtccccggc cgggcgagcc gccactgacg
       61 cctcgaaggt gtggtcggcc tgccagtacc gcagcacggc gagctcaacg gcgggcaagg
      121 acggttgggc cgagacgggc tccgcggcaa ccttcgggta gaccatggtg gtatgcgctc
      181 ctcagggcag tggcattgcc ggtcgttcca gtgcagtggt ttcctgctcg ctgcattggg
      241 gacgacccgc aactcacgcc acggaccgcg gtaccacccc gcttgccacc tgcgctggca
      301 ggcggccact cattgagccg gcgatgacgg gccggacccg tccggttcta ctggaggctc
      361 atccaccggg tcatccggtg cgtggacttg tctcgttctt ccggaggctc cccggtgatg
      421 gccggatcgg tgcctttggc gccatcttac ccgggctgcg ccagcgattt gtgcccggct
      481 gacctcagct tctgcccgtg gccgcgctcg cggcccggtg ccggcgggct agtgcagcat
      541 ccggccggca ccggtcacac ggtgtgaaac ccagctccct ggcctcacgc accggcagcc
      601 gctcaccttg ctcgtggtcg ggccaggtgc agcgggtgag atggtaacgc gggtgctcgt
      661 cgacaacgag cacctcgtca ttcaactcgt agaccaccaa cagatccgcg gcatcagtgt
      721 cttcctcacc gggctcgccg ggttcgtcct gctcgccagc ctcgatctcc tccccggcat
      781 gctccgcagc ggtcaccggc gctggctgcg gcgatggttc actcgggtcg gcgtcgctgg
      841 caggatcaac tggcagggca tcggccggct cgtcgtccag tagcacaaca ggctcggccc
      901 cgggctcggc ccggcgccgc cgccaccgca tgacgatcat ggcggcgacc acagcactga
      961 gcgcgaccga tacccaagcc aaagccagct ggccagtgcc aagcgcggcg atgagcagcc
     1021 ccaccgccac gagtagcaaa accacggtaa gacgcatcac cgctcaccca tgcgttccgg
     1081 gcccaacggt ggtgggggat ggttcaccgg gctgaaccgg cgctcccggc gcgcgtcagc
     1141 taccggcttc ggcacgcgcg ccgaacccgg aggtgacgaa accctgctgg ctacgcaccg
     1201 tgtccgccgg tgcggcagat ccgcgcccgt ccagctctcg aagttgtgac tccagatagg
     1261 ttttgagacg ggttcggtac tcgcgttcaa acgtgcgcag ctcgtcgatc ttcttctcca
     1321 gggtggtctt ctcctggttg atcgagctga ggatctcggt gtgtttgcgc gcggcgtcgc
     1381 gctccagcga cgaagctttt tcgcgggact gccgttccag ggtttcggcc ctggtacggg
     1441 cgtcactcag catggtttcg gctcgtgttc tggcctcgtt gaccataccg tcggccttgg
     1501 ctctggcatc ggagagcaac tgctcagact tggtccgggc ctcgcccaac atcccatccg
     1561 cttcggcctt ggcctcaccg gtcaaccggt ccgccatctc ctgagccagg cccagcacct
     1621 tggcagcttg gacgtggtgg tcaccacctg gtgcggtctg ctcgaccatc ggcggcggca
     1681 ccggggccat caccggccgt ggcggctcca gcggacgaag attacgtccg gtatcgatcg
     1741 gcgcagcact gagctggcga tcgagctcct caacctggtt gcggagatcg ttgttttcct
     1801 cgatcaagcg agccaactcg gcctcaacca ggtcgaggaa ggcgtccacc tcgtcctcgt
     1861 tgtagccccg cttgccgatc ggcggcttac tgaacgcgac gttgtgaacg tcggcggggg
     1921 tcaacggcat cggatcacct cacgcactcc tcgactatgc acacgctgtg tgttcggccc
     1981 caagcccggc ggccgctacg tccttggatc gatcactcgc atcagtatga agaccacgag
     2041 cagcagaacc ataatggaca ggtccaggcc cacgcctccg atgcggatgg gtttgaccac
     2101 ccggcggagt gcccggaccg gcggatcagt catcgtatag accgactcaa gcgcgattgc
     2161 cacccctccg accggctgcc agcttcgagc gaaggcgcga actagttcca ccacaatacg
     2221 cgctgtgagc agcaaccaga agaagaaaag caggtagtag accaccaacc gcaccacgtc
     2281 cacggcgaca ctctgccatc gg
//
LOCUS       DATADN010000843         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_560708, whole genome shotgun sequence.
ACCESSION   DATADN010000843 DATADN010000000
VERSION     DATADN010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560708"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VHH53_10925"
     CDS             <1..181
                     /locus_tag="VHH53_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290679.1"
                     /translation="HGEADPAHAITARIRYGDLHSWQQIRTPEDVAAVLARAEQIAFD
                     YFGRIFPVVDLVSRP"
     gene            178..1617
                     /locus_tag="VHH53_10930"
     CDS             178..1617
                     /locus_tag="VHH53_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator"
                     /protein_id="HEX2290680.1"
                     /translation="MTTTVPQRGLPDTTGATATAVHRAARPGYRRWLDHVSSVGGCER
                     PIRLAGHLHTINPTTGEVLSSRSTETLPDGVIYVPCGDRRASVCPACAETYRADTYHL
                     IRTGLVGGKGIPDTVATHPVVFATFTAPAFGPVHSRVIDRTTGKVKPCRMRRTVERCR
                     HGRVLVCRQRHDEDAKCVGQPLCPQCYDYAHHVVWNAWASELWRRTTITATRNLRDLA
                     QAHGVQLRLSFAKVAEYQARGLIHFHALIRLDALDPTDPDTLVAPLAGITTADLDQVI
                     TRAAATTRVITPPHPHNPDGWLLTWGSQLDIRPVSFAGELTDTAVAGYLAKYATKATE
                     ATGHVSQRITTETIGTYANTATHAGRLVRTCWILGRRPDDAHPADWKPSYGRLRRWAH
                     MLGFGGHFATKSRRYSTTRAALKTIRREWRRAHQSEHHTAEETTLVVGALIFAGIGYR
                     SAGDRWFALTVAAKTREQRQIAKEERSAA"
     gene            1835..2098
                     /locus_tag="VHH53_10935"
     CDS             1835..2098
                     /locus_tag="VHH53_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2290681.1"
                     /translation="MAHTQSTVNPELPALISIPAAARLLGLSRASAYRYAAAGELPTK
                     RYGRRVYVIRARLADVLGDGSSAPWKGKEERTANEWEGTSAAQ"
     gene            2085..>3355
                     /locus_tag="VHH53_10940"
     CDS             2085..>3355
                     /locus_tag="VHH53_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX2290682.1"
                     /translation="MRRSKAIAGTVYRRGNRWAFMFDLGPDPLTGKRRQSAKSGYDTE
                     AAAWAALADANHELTAGRHVRASKRTVREFFNEWLPALVNDPSVKPTTEANYEDYARA
                     YVLPHIGDRPLQSIEPEVLTALYQHLARAGRRKADTNSVMYAHWKTKTDAGERVRPAD
                     LAEIAAVTYSAAWNALRRYQAGRTPKTNGDGLAPKTVRNVHIMLRRAFSDAVAMQYIS
                     RNPATAVKAPAFRRKPHTTWRPEQLTRFLAEAKSERLYAMWVLFATTGMRRSEAVGAR
                     CDAVDLDHLTIAVHDTRVVVRGRAEDSDGKTRTSRRILALDPATAAVLRAHLEQLAAE
                     RTEWGTSYQDHGKLFCWEDGRPIHPDTITEHFNRIVDRANLPAIELHAVRHTYATTAL
                     RAGINPKLVSARLGHSSVAFTLDTYTDNVPEL"
BASE COUNT          696 a         1209 c          943 g          507 t
ORIGIN      
        1 ccacggcgaa gccgacccgg cccacgcgat cacggcccgg atccgctacg gcgatctcca
       61 cagctggcag cagatccgca caccggagga cgtcgcggcg gtcctggccc gggccgagca
      121 aatcgctttc gactacttcg gccggatctt ccccgtcgtc gacttggtga gtcgcccttg
      181 acgactaccg tgccgcaacg cggcctgccg gacaccactg gcgcaacggc cactgcggtg
      241 caccgtgcgg cccggcccgg ttaccgccgc tggcttgatc acgtgtcctc ggtcggtggc
      301 tgtgagcgcc cgatccgact ggccggacac ctgcacacca tcaatcccac caccggggaa
      361 gtcctgagct cacgcagcac cgaaaccctc cccgacggag tcatttacgt gccctgcggg
      421 gaccgccgcg ccagcgtgtg cccggcgtgc gccgagacct accgggccga cacctaccac
      481 ctcatccgta ccggactcgt cggcggcaag ggcatccccg acaccgtcgc gacacatccg
      541 gtggtgttcg ccaccttcac tgcccccgcc ttcggcccgg tccactcgcg agtgatcgac
      601 cgcacgaccg ggaaggtcaa accctgccgg atgcgccgga cggtggaacg ctgccgccac
      661 ggccgggtac tggtctgccg gcaacgtcac gatgaagacg caaagtgtgt ggggcaaccg
      721 ttgtgcccgc agtgctacga ctacgcccac cacgtcgtgt ggaacgcctg ggcctcggag
      781 ctgtggcgcc ggaccaccat taccgccacc cgcaatctgc gtgacctcgc tcaggcccac
      841 ggtgttcagt tgcggttgtc gtttgccaaa gtcgctgaat accaagcccg cgggctgatc
      901 cacttccacg ccctgatccg cctcgacgcc ctcgacccca ccgacccgga caccctcgtc
      961 gccccactag cgggcatcac gaccgcagat ctggaccagg tcatcacccg ggccgcggct
     1021 accacccgcg tcatcacccc accgcacccc cataaccccg atggttggct ccttacctgg
     1081 ggcagccaac tcgacatacg ccccgtctcg ttcgccggag agctcaccga taccgccgtg
     1141 gccggttacc tggccaaata cgccaccaag gccaccgaag ccaccggcca tgtctcccag
     1201 cggatcacca ccgagaccat cggcacctat gccaacaccg ccacccatgc cggacgcctg
     1261 gtgcggacct gttggattct tggccgccgc cccgacgatg cgcatcccgc tgactggaaa
     1321 cccagctacg gcaggctgcg ccgctgggct cacatgctcg gcttcggcgg gcacttcgcc
     1381 acaaaatccc gccgctactc caccacccgc gccgcgctca aaaccatccg ccgcgaatgg
     1441 cgacgtgccc accaatccga acaccacacc gccgaagaaa ccactctcgt cgtcggcgcc
     1501 ttgatcttcg ccgggatcgg ttaccgcagt gccggagacc gatggttcgc cctgaccgtc
     1561 gcagctaaga cccgcgagca acgacaaatc gccaaggaag aaaggagtgc cgcatgacat
     1621 cacccggaaa ttggaaggag gaaagctgtg aacgttgtgg tctacaccat cgaggaagcc
     1681 gcacaggtac tcaagatcgg tcgatggaag gtcgtcgact tgatccgtac caatcagctt
     1741 cgctcagtaa agatcggtgg cctgcgtcga atcccgcacg ctgccattga taagtacatc
     1801 actcgactca tggaggcgat tggagaaaca cctaatggcg cacacacaat ccaccgtaaa
     1861 cccggaattg cccgcactga tctcgatacc cgccgctgcg cggttgctcg gtctcagccg
     1921 ggcctctgcc taccgctatg ccgccgccgg agagttgccc accaaacggt acgggcggcg
     1981 ggtatacgtc attcgcgcac gactcgcgga cgtgctcggt gacggcagtt cagccccctg
     2041 gaagggaaag gaagaacgga cagctaacga gtgggagggc accagtgcgg cgcagtaagg
     2101 caatcgccgg cacggtctat cggcgtggca atcggtgggc cttcatgttc gacctcggac
     2161 cagacccgtt gaccggaaaa cgacgtcaaa gcgcgaagag tggttacgac accgaggccg
     2221 ccgcatgggc cgcactcgcc gatgcgaatc atgaactcac agcgggcagg cacgtcaggg
     2281 catccaaaag gacggttcgt gaattcttca acgagtggct ccccgcgctg gttaatgatc
     2341 caagcgtcaa accgaccacc gaagcgaact acgaagacta tgccagagca tacgtcctgc
     2401 cccacatcgg cgaccggccg ttacagagca tcgaacctga agtgctcacc gccctctacc
     2461 aacacctcgc aagggccggt cgacgcaagg ccgacaccaa ctccgtgatg tacgcccact
     2521 ggaagaccaa gaccgacgcc ggtgaacggg taagaccggc tgatctcgcc gagatcgcgg
     2581 cagtgaccta ctcagcagcg tggaacgcgc tccgccgcta tcaggctgga cgcactccaa
     2641 agacgaacgg agacggctta gcccccaaaa ctgtccgcaa cgtgcacatc atgctccgac
     2701 gagcattcag tgacgccgtc gccatgcagt acatctcgcg taacccggca accgcagtga
     2761 aggcccccgc cttccgacgc aaacctcata cgacatggcg cccggagcag ctaacccgct
     2821 tccttgcaga agccaagtca gaacgcctct acgcgatgtg ggtcctgttc gcaacaactg
     2881 gcatgcggcg ctctgaggca gtaggagcac gctgcgatgc cgttgaccta gaccatctga
     2941 caatcgcggt gcatgacaca cgcgtcgtgg tccgcggtcg agcagaggac tcagacggga
     3001 agacccgaac tagccgacga atcctggcgc tagaccctgc cacggcggcg gtgctgcgtg
     3061 ctcacctaga gcagctcgcc gccgaacgca ccgagtgggg aacgagctac caggatcatg
     3121 ggaagttgtt ctgctgggag gacggccgac cgatccaccc cgacaccatt accgagcact
     3181 tcaaccgcat cgtcgaccga gcgaacttac cggccatcga actgcacgcc gtccgccaca
     3241 cctacgccac gactgcactg cgcgcgggga tcaacccaaa gctcgtcagt gcccgcctag
     3301 gccactcctc ggtcgccttc acactcgaca cctacaccga caacgtcccg gaact
//
LOCUS       DATADN010000844         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1017150, whole genome shotgun sequence.
ACCESSION   DATADN010000844 DATADN010000000
VERSION     DATADN010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017150"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..411
                     /locus_tag="VHH53_10945"
     CDS             130..411
                     /locus_tag="VHH53_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2290683.1"
                     /translation="MNQVEVADWRRRGACGTHDPDQLFVTGAAQRQAKIMCTGCPVRL
                     HCLAEALDRRIEFGVWGGMTERERRALLHRRPDIRNWAKLLKGASQHHA"
     gene            complement(408..1568)
                     /locus_tag="VHH53_10950"
     CDS             complement(408..1568)
                     /locus_tag="VHH53_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX2290684.1"
                     /translation="MTPPPPLLNIDALLDNRSTRVVVCCGAGGVGKTTTAAALALRAA
                     EQGRSVVVLTIDPARRLAQSLGLRELTNNPRRVAPTEEIEAAGGQLHAMMLDMRRTFD
                     EMVQAHASPERAVQIIANPFYQTISSSFSGTQEYMAMEKLGQLVAAGTWDLVVVDTPP
                     SRSALDFLDAPQRLSSFLDGRMIRLLSGPVRAGGRGVRRLVGAGFGLFARAVSAILGG
                     QLLADASAFVQAFDTMFGGFRERADATYKLLRAPGTAFVVIATPETDAVREAAYFVDR
                     LATESMPLAGLLLNRTHPVLADLPAAQAETVAETLEQGGKAPLAAAVLRLHADRVTLA
                     RRERRLLARFRRAHPAVAVATAPALAADVHDLPGLREIGRLLTTAQPAVSRD"
     gene            complement(1565..>2421)
                     /locus_tag="VHH53_10955"
     CDS             complement(1565..>2421)
                     /locus_tag="VHH53_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2290685.1"
                     /translation="RQGIAHLFDAPPLPYQERRIAVAPGRGEVRALAVDAEEALLEYF
                     AMFYNLGFAGRTLRRMGAIEFATTLAPGLRDVLLTGKVKEATTRANKGIHIYDAVVLD
                     APPTGRVVKFLDVTRAMADLAKAGPIHHQSEGVVRLLHSADTAVHVVTLLEDLPVAET
                     LETVAELDLADLRPGAVLVNRARPPRLSNRSVSAAANGRVDADRIRAGLLAASLDLDP
                     AVLDGLVAETVEHAVRVDAEQQARTKLADAGMPLLELPELTEVGVDGVDLGGVYELAE
                     VLRAQGVR"
BASE COUNT          382 a          850 c          824 g          365 t
ORIGIN      
        1 ctacggtcgt gcctgcccca ggggcaaggt caagcactcc cccacacgga accggcgcta
       61 tccacgttgc gtcaaagccc cggatttgcg ctgagtgaga cgacgagagg ccggcgagcc
      121 atgggcacca tgaatcaggt ggaggttgcc gactggcggc gacgcggtgc ctgcgggacg
      181 catgatccgg accagttgtt cgtcacgggc gctgcgcaac gtcaggccaa gatcatgtgc
      241 accgggtgtc cggtgcggct gcactgcctg gctgaggcgc tggaccgccg gatcgagttc
      301 ggcgtgtggg gcggcatgac cgaaagggaa cgacgtgcgc tgctgcaccg tcgcccggat
      361 atccgcaatt gggcgaagct gctcaaggga gcctcgcagc accacgctta atcacggctg
      421 acggcgggct gcgccgtggt caacagcctg ccgatctcac gcaagcccgg gaggtcgtga
      481 acgtcggcag ccagcgcggg tgcggtggcc accgccaccg ccggatgggc ccggcggaac
      541 cgcgccagca gccgacgttc ccgtctggcg agggtcaccc ggtcggcgtg cagccgcagc
      601 accgctgcgg ccagtggcgc cttcccgccc tgttccagcg tctcggccac cgtttcagcc
      661 tgcgccgccg ggaggtcggc gagcactgga tgcgttcggt tgagtaacag cccggccagt
      721 ggcatcgatt cagtggccaa ccggtcgacg aagtaggccg cctcgcgtac cgcgtcggtc
      781 tccggtgtag cgatcaccac gaacgcggtt ccgggcgcgc ggagcagttt gtatgtggcg
      841 tcggcacgtt cccggaagcc gccgaacatg gtgtcgaagg cttgcacgaa cgccgaggcg
      901 tcagccagca gctgcccgcc gagaatcgcc gacactgccc tggcgaacag cccgaatccg
      961 gcgcccacca gccggcgtac gccccgcccg ccggcccgta ctggtccgga cagcagccgg
     1021 atcatccgcc cgtccaggaa cgacgagagg cgctgcgggg cgtccaggaa atccagcgca
     1081 gagcgtgaag gtggggtatc gaccacgacg agatcccagg taccggccgc gaccagctgc
     1141 cccagctttt ccattgccat gtactcctgc gtgccggaga acgacgagga aatggtctgg
     1201 tagaagggat tggcgatgat ctggacggcc cgctcggggc tggcatgtgc ctgcaccatc
     1261 tcgtcgaagg tgcgtcgcat gtccagcatc atcgcgtgca gctggccgcc cgccgcctcg
     1321 atctcctcgg tcggtgccac ccggcgaggg ttgttggtca gctcccgcag ccccagcgat
     1381 tgcgccaacc gtcgcgcggg gtcgatggtg agcaccacca ccgaccggcc ttgttcggct
     1441 gcgcgcagtg cgagcgccgc ggccgtcgtc gtcttgccca ccccacctgc gccgcagcac
     1501 accaccacac gggtggaacg gttgtcgagc agcgcgtcga tgttgagcag aggcggtggt
     1561 ggcgtcatcg cactccctgc gcgcgcaaca cttcggctag ctcgtagaca cccccgaggt
     1621 cgaccccgtc caccccgact tcagtgagtt cgggcagctc gagcagcggc atgccggcat
     1681 ccgccagctt ggtgcgagcc tgctgctcgg catccacccg caccgcatgc tccacggtct
     1741 cggcgaccag gccgtcgagc acggccgggt ccaggtccag gctggcggcc agcaatccgg
     1801 cgcggatccg gtcggcatcg acccggccgt tggcggcggc actgaccgaa cgattggaca
     1861 gccggggtgg ccgggcccgg ttgaccagca cggcacctgg gcgcaggtcc gccagatcca
     1921 gctctgccac ggtctccaag gtctcggcca ccggcaggtc ctcgagcagg gtcaccacgt
     1981 gaaccgcggt gtccgccgag tgcagcagcc gcaccacccc ttcgctctgg tggtggatcg
     2041 ggcccgcctt tgccaggtcg gccatcgccc tggtgacgtc gaggaacttc accacccgcc
     2101 cggtcggcgg ggcatccagt accactgcat cgtagatatg gatgcccttg ttcgcgcgcg
     2161 tggtggcctc cttcaccttg ccggtgagca gcacatctcg cagcccaggc gccagcgtcg
     2221 tggcgaattc gatcgcgccc atccggcgca gggtccggcc cgcgaaaccc aagttgtaga
     2281 acatcgcgaa gtactccagg agcgcctcct cggcgtctac cgccagggcc cggacctcgc
     2341 cgcggccggg ggcgaccgcg atgcgccgct cctgataggg cagcgggggt gcgtcgaaga
     2401 ggtgcgcgat gccctgccgc c
//
LOCUS       DATADN010000845         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1017276, whole genome shotgun sequence.
ACCESSION   DATADN010000845 DATADN010000000
VERSION     DATADN010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017276"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..903
                     /locus_tag="VHH53_10960"
     CDS             142..903
                     /locus_tag="VHH53_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2290686.1"
                     /translation="MVASDTDLTIDQLAARAGMTVRNVRSHASRGLLPSPQLRGRTAY
                     YGAEHLARLQLIAGLQQQGFSLAAIEAVVRQTPARSAEQALAFYRGVLAPWQPEDPVE
                     MSYAELAQWLGVEPQPTTLDQLHAEGTLQSLGPDRVRIINPGLLRAGAQAVRLGMPVE
                     AVMAMRPELLKHSRAISELFVALFRDTVWRDYVGRGMPQGEWSEVHGVVEALQPVAAQ
                     ALLAAFRSTMPEAIEVFVRDECDRLAEGADRNANV"
     gene            946..2112
                     /locus_tag="VHH53_10965"
     CDS             946..2112
                     /locus_tag="VHH53_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290687.1"
                     /translation="MVFHNTMTSIQGLVTTATSGLAAAQEVRPAGGAGLDQIIIASTV
                     ATVLSAGLLLLGYGHRTGRIPILRRLAFETQRWPFSAGQPGWVELPSTLGFFSLITAL
                     LGMYWDIAVHIADGRDTGPLANIAHYPIMLGLFGIFAAGVLAIVLPVGERPGPAAVRI
                     TRDWYAPVGGILMAAAGFFGLLGFPLDDVWHRLFGQDVTLWSPTHLMLIVGGGLSLVG
                     LAVLEREGRLAHGYDEVTALGLFVRRGMIMGGLLIGLSVFQGEWDFGVPQFRIVLQPL
                     LIAMAAACALVAARLWMGRGGALFAVGFYVGVRGGVALIVGPFLAEPFPTIPLYLAEA
                     LCVELAALVLARRALLCGAVSGLLIGQPASLPNTPGPSWPTGYPGPPTCSSKAC"
BASE COUNT          297 a          692 c          760 g          421 t
ORIGIN      
        1 ttggcgccgc caatcgatgc gtgtgccgag gcattgcgca tcgcgcagat gatcgagcct
       61 cggttgccga aagcgtgagc gggcgcaccg gccgatcttt acggtatttg atgttacatt
      121 ccagacggta atatccagcg cgtggttgcc tccgacactg acttgaccat cgatcagctt
      181 gccgcgcgtg ctgggatgac ggtccgtaac gtccgttcgc acgccagccg cgggttgcta
      241 ccgtcgccgc agctgcgtgg tcgcaccgcc tactacggcg ccgagcatct cgcgcggctc
      301 caactgatcg ccgggctgca gcagcagggc ttctcgctgg ccgccatcga agcggtcgtg
      361 cggcagactc cggcgagatc ggccgagcag gcactcgcct tctaccgggg cgttctcgct
      421 ccttggcagc ctgaggatcc ggtggagatg tcctacgccg aactggccca atggctgggc
      481 gtcgagccgc agcccacgac gctggaccag ttgcatgccg agggaacctt gcagtccttg
      541 gggcccgacc gggtgcggat catcaaccct ggactgttac gtgccggggc tcaagcggtg
      601 cggctgggaa tgccggttga ggcggtgatg gcgatgcgac ccgagctgtt gaagcactcc
      661 cgggcgattt ccgagctgtt cgtggcgctg ttccgcgaca ccgtgtggcg ggactacgtg
      721 gggcggggca tgccacaagg cgaatggtcc gaggtccacg gtgtcgtcga agcgctgcag
      781 ccggtggccg cgcaggcgct gctggcggcc ttccgctcca cgatgcccga ggcgatcgag
      841 gtgttcgtcc gggatgaatg tgacaggttg gccgaggggg ccgaccggaa cgccaatgtc
      901 tgacggcgtt caatgtgaca tggatacgtg acaacatagc ggcagatggt gttccataac
      961 accatgacat cgatccaggg gctggttacc accgccacct cggggttggc cgccgcccag
     1021 gaagtgcgcc ccgctggtgg cgccgggctc gaccagatca tcatcgccag caccgtggcg
     1081 accgtgctca gcgcgggctt gctgctgctc ggctacggcc accgcaccgg ccggatcccg
     1141 atactgcgca ggctggcctt tgagacccag cgctggccgt tcagcgcggg gcaacctggt
     1201 tgggtcgagc tgccctcgac actggggttc ttctcgctga tcaccgcgtt gctggggatg
     1261 tactgggata tcgcggtgca catcgccgac ggccgagaca ccggtccgct ggccaacatc
     1321 gcgcactacc cgatcatgtt gggcctgttc ggtattttcg ccgccggcgt gctggccatc
     1381 gtgctgccgg tgggggagcg gcccggcccg gctgcggtgc ggatcacccg cgactggtac
     1441 gcgccggtcg gcggaatctt gatggccgcc gcggggttct tcgggctgct gggctttcct
     1501 ctcgacgacg tctggcaccg gctgttcggc caggatgtga ccctgtggag tcccacccac
     1561 ctaatgttga tcgtcggcgg tgggttgtcg ctggtgggcc tcgcggtact cgagcgggaa
     1621 gggcgcctgg cgcacggcta cgacgaggtc actgcactgg gccttttcgt ccggcgtgga
     1681 atgatcatgg gtggcctgct catcgggcta tcggtgttcc agggcgaatg ggacttcggc
     1741 gtgccccagt tccgcatagt gctccagcct ttgctcatcg ccatggctgc ggcgtgtgcc
     1801 ctggtggctg ctcggctgtg gatgggccgc ggcggcgcac tgttcgccgt gggcttctac
     1861 gtcggcgtgc gcggcggggt cgcattaatc gtcgggccct tcctcgcaga acccttcccc
     1921 actatcccgt tgtacctggc agaggcgctg tgtgtggaac ttgccgcttt ggtgcttgcc
     1981 cgccgagcgc tgctgtgcgg cgccgtcagc ggcctgctga tcggacagcc ggcttcgctg
     2041 ccgaatacgc ctggacccag ctggcctacc ggataccctg gacccccgac atgctcgtcg
     2101 aaggcatgct gatggccctg accggcgggg tcgcgggcgg ggtcgccggt gccctgctgg
     2161 cgcagggtct
//
LOCUS       DATADN010000846         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1467429, whole genome shotgun sequence.
ACCESSION   DATADN010000846 DATADN010000000
VERSION     DATADN010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467429"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VHH53_10970"
     CDS             complement(<1..504)
                     /locus_tag="VHH53_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290688.1"
                     /translation="MTAPHSSTSSDDTSRKIDLNLTHVAATALAAVTAAVLGSELGAA
                     GTLIGAAAASVITTVGTAVYKASLERRRERVRSLAHRTRPLPTSPERSRIERVHSPAV
                     SAPLGNGQRTDGVRESQPPDRSRRFATLRWGAMAVGALGAFLLAMMVITGFEWASGGT
                     VGGNGNGT"
     gene            complement(935..1195)
                     /locus_tag="VHH53_10975"
     CDS             complement(935..1195)
                     /locus_tag="VHH53_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290689.1"
                     /translation="MSAVAGRWTSQDTAQFVVSELVANAVEYACTGVGVTLEWGPQGL
                     RIAVRDSSPVGFPGPVERSTDPLGQVRGRGLELELVDTLATA"
     gene            complement(1238..1621)
                     /locus_tag="VHH53_10980"
     CDS             complement(1238..1621)
                     /locus_tag="VHH53_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290690.1"
                     /translation="MSSALDIAAYRHGQVVVVHPRGLLTVRTGTELRRVLTKELTGHG
                     RVVVDLDGFSVGRASCVTIFPAVLTQCGGWPAAKLALCRPEPGMAQALAARRVSALVP
                     VYHFVEAQAAIDSRPPGAGTGAATF"
     gene            1904..2293
                     /locus_tag="VHH53_10985"
     CDS             1904..2293
                     /locus_tag="VHH53_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2290691.1"
                     /translation="MVTATLSCPRPNTTVCTVTGRIDVVTASVLSDTLIEAVHDERPH
                     LVIDLSAVDFVDSAGLHAIFESLDGYDIGGHVAVVVDPNSEAVTPLEIAALDEFLDRH
                     GDLTRALQACARASISTAGRHRAKAMD"
     gene            2407..3279
                     /locus_tag="VHH53_10990"
     CDS             2407..3279
                     /locus_tag="VHH53_10990"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2290692.1"
                     /translation="MGAGGSKVNWQQSQDALRDEVARVTALLRSIQDPGIPAVGQWNV
                     GELAMHLSQVWIAVPGLARRNLSRLYEAIPSIADVAGDSLMQDMCELDDLTVLGVKSD
                     SQRDPAVLADRIEQRAQEYFAECAGHSPDELRPWFVEGSTLPLSAMTGHLLNETVMHG
                     YDIARATGHKWRIEPAHAAMVVRQFFVPVLRTLDPRVFVNGEKAAGLRATYDVRLRGH
                     DRLHFIFDDGSLRVEEATSRPVDCHISADPAAFVMVFWTRQNQWSAIAQGKLMAWGRK
                     PWLGPRFRPLLRSL"
BASE COUNT          658 a         1132 c         1130 g          617 t
ORIGIN      
        1 ggtgccgttg ccattaccac caacggtgcc accactggcc cactcaaagc cagtgatgac
       61 catcatagcc agcaggaagg caccgagtgc ccccaccgcc atcgctcccc accgcagcgt
      121 ggcaaaccgt cgggatcggt ccggtggctg cgattcgcgg acaccgtcgg tgcgctgtcc
      181 gttgccgagc ggggcgctca ccgctggcga gtggacgcgc tcgatcctgg acctctccgg
      241 agaggtgggc agcggccgag tgcggtgagc aagtgaccga acccgttccc gtctgcgttc
      301 cagggatgcc ttgtacacgg cggtgcctac ggtggtgatc acactcgctg cggcggcccc
      361 gatgagggtg ccggcagcgc cgagctcgga accgagcacg gcggcggtca ccgcagcgag
      421 ggcggtcgcg gcgacgtggg tcaggttcag gtcgatcttg cggctggtgt cgtctgagct
      481 agtggaagag tgcggcgcag tcataatctt cggtatgttg cgggtctaga tgatcaatgg
      541 tcatcgggac gtcgatcctt ggccgactac ggtaacgcgg accctggcca tccccaatcg
      601 gggtaattac cgcaggagtg gcgccgtagt gcgggtcggt ggaccgcagt cgccgccaac
      661 tcgatcgtga gttcgcgaaa gcgagtcgat ttattcccag gagtgtcaag ggcggactca
      721 agccgaccgg gccaagtcgt gtccgtagaa cctcatagtg cgtcagtgac ctccctgagg
      781 gtggcaagat actcaaagac tagtcgtcat cagatttccg ccttaaccgg gagctccggc
      841 acgaggtgtg ggacctgccg accggaatca ggtccgctgg ctttggtggt tatcccagcc
      901 cacatggtat tgccgtcggg ttggacgtcg acgcctatgc cgtggcgagg gtatccacca
      961 gttccagttc cagtccccga ccgcgtacct gacccagcgg atcggtcgag cgctccaccg
     1021 gtccgggaaa gcccaccggt gatgaatccc gcacagctat tcgcaaacct tgcggacccc
     1081 actccagagt cacgccgaca ccggtgcacg cgtattcgac cgcgttggcc accaactcac
     1141 tgaccacgaa ttgggcggta tcctgcgatg tccatcgacc tgccacagcg gacacatctg
     1201 gcggaccagc cgccgagcgg tcaccggggc accggcgtca aaaggtagct gcacccgtac
     1261 ccgcaccagg aggccgcgag tcgatcgcgg cctgtgcctc gacgaagtga tacaccggta
     1321 ccaatgccga cacccggcgg gccgcaagcg cctgagccat tcccggctca ggccggcaca
     1381 gcgcgagttt cgcggcaggc caaccaccac actgggtcag caccgcggga aaaatggtca
     1441 cgcacgacgc gcgccccaca gagaagccgt cgaggtcgac cacgacacgg ccgtgaccgg
     1501 tcagttcctt ggtcaacacc cggcgcagct ccgtcccggt gcgtaccgtg agcaaccccc
     1561 gcgggtgcac cacgacaacc tgcccgtgac ggtaagccgc tatatcgagt gccgaactca
     1621 tcgacctccc cactccgcct acactgcgtg ccgaggcgtg cgcgtagagc ttacgacgct
     1681 acgcaagagc gttcgccctg aaaacgacat aaaggacttt ggatagcatc ctcacgccct
     1741 ccaccatggc gcgccggtgc tgactgtcag gatttcttga cacccccgtt gacgcacata
     1801 gcagagcgtg tacggtccga tctcagactg taacgcccgg gggaggagtc gacccgtgac
     1861 acgcgagcag cttgacacca tgccgccgcc acttggtgga cacatggtga cagcaacatt
     1921 gagctgtccc cgaccgaaca cgacggtgtg cacggtaacc ggaaggatag acgtggtcac
     1981 cgcgtccgtg ctgtccgaca cgctcatcga agcggttcat gatgagcggc cgcatctcgt
     2041 gattgacctg tccgccgtcg atttcgtgga ctcagccggc ctgcacgcta tcttcgaatc
     2101 cctcgatggg tacgacatcg gtggtcacgt cgccgtggtg gtggacccga actctgaggc
     2161 cgtcaccccg ttggaaatcg cggcgctgga tgagttcctc gaccggcatg gcgatctcac
     2221 gcgagccctg caagcctgtg ccagggcttc gatctccact gcgggacgcc accgagcgaa
     2281 agccatggac tagcctcgac gacccaccgg aaagtcggtc gtccagcgga tgtggatagc
     2341 tgcgattcgg ctctacagtc acggctgtgc gcaccggggg atgagcgacg agtcaaaggg
     2401 ctgagcatgg gcgcaggcgg gtctaaggtc aactggcagc agagtcagga cgcactgcgt
     2461 gatgaggttg cgcgggtgac ggccctgctg cggtccatcc aagatccggg gattcctgcg
     2521 gtgggtcagt ggaacgtcgg tgagctggcc atgcatttgt cgcaggtctg gatcgccgtg
     2581 cccggcctgg cgcgacggaa cctctcacgg ctctatgagg cgattcccag cattgccgac
     2641 gtggccggcg attccctgat gcaggacatg tgcgaactgg atgacctgac ggtgctgggg
     2701 gtgaaatctg attcgcagcg cgatccggct gtcctcgctg accgcatcga gcagcgggcg
     2761 caggagtact tcgcggaatg tgcagggcac tctcccgatg aactccgtcc gtggttcgtc
     2821 gaaggcagca cgctgccctt atcagccatg accggccatc tgttgaacga gacggtcatg
     2881 cacggttacg acatcgcgcg cgcaacgggc cacaagtggc ggatcgagcc ggcccacgca
     2941 gccatggttg tgcggcagtt cttcgtgccg gtgctgcgaa cgctcgaccc tcgggtgttc
     3001 gtgaacgggg agaaggcagc cggtctccgg gccacctatg acgtccgcct tcgagggcac
     3061 gaccggctcc atttcatttt cgacgacggg tcgctcaggg tcgaggaggc gacctcgcgc
     3121 cccgtcgact gccatatctc ggccgatccc gctgcgttcg tgatggtgtt ctggacccgc
     3181 cagaaccagt ggagcgcgat cgctcagggc aagctgatgg cgtggggccg caagccgtgg
     3241 ctggggcccc ggttcaggcc gttgctgcgc agcctctagt cactcgcaca gtgcattcga
     3301 tgcccgggtg accgtgacac ccgcgcatac gtcatctact cgatgtgcaa cacctggatc
     3361 cgttcctacg gttaccgctg cgcaactcga gttgaacgac ggacccgagg gagctgactt
     3421 ggccacgaac ggattgaaag ccgactggca gcacggtcag aatgcactcc gcaaagaggt
     3481 gcagcgagtc accgccctgc tgaggtccat cgaagatcca acgcctcatg cggtggg
//
LOCUS       DATADN010000847         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_561185, whole genome shotgun sequence.
ACCESSION   DATADN010000847 DATADN010000000
VERSION     DATADN010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561185"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VHH53_10995"
     CDS             <1..540
                     /locus_tag="VHH53_10995"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2290693.1"
                     /translation="GRERTGPAVEWIEADVEDLPLPDMTVDVALSAFGVIFAHHPEVA
                     LSELHRVLTPGGRLGLTAWTIDGWIAERARIVRQFVPPDPAAPDSLSWGDAGVLERRL
                     STEFTDVRIERRSLPWHFDSGAALTEFLTAHSPAHVAAMQAAGDRADEMVAAIERHAS
                     PDGGPVRIEAEYLLALAHS"
     gene            complement(617..769)
                     /locus_tag="VHH53_11000"
     CDS             complement(617..769)
                     /locus_tag="VHH53_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290694.1"
                     /translation="MRQSGPLVRMAAQPDSALGCALASLVPLMGRTGTAVAVAGIDNS
                     AQVQWW"
     gene            834..>1704
                     /gene="purD"
                     /locus_tag="VHH53_11005"
     CDS             834..>1704
                     /gene="purD"
                     /locus_tag="VHH53_11005"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX2290695.1"
                     /translation="MRVLVIGAGAREHALVLALSRDPSVTALACAPGNAGTAALAQQH
                     GVDVTDPAAVAQLATSWPADLVVIGPEAPLVAGAADAVRAAGIACFGPSAGAAQIEGS
                     KAFAKDVMAVAGVPTARSELVNNPAQLDAALSRFRPPWVVKDDRLAAGKGVVVTEDVE
                     VARVHALTLLDGGHPVLLESYLEGPEVSLFCLVDASAVVPLLPAQDFKRVGEADVGPN
                     TGGMGAYAPLPWAPESLATDVTEQIVQPVVDELARRGAGFSGLLYAGLALTAQGAQVI
                     EFNCRFGDPETQAV"
BASE COUNT          256 a          560 c          601 g          287 t
ORIGIN      
        1 ggccgggagc gtaccgggcc ggcggtggag tggatcgagg ccgacgtcga ggacctgccg
       61 ctgcctgaca tgaccgtgga cgtcgcactc tccgcattcg gtgtgatctt cgcacaccat
      121 ccggaggtgg ctctttccga attgcatcgc gttctgacgc ccggtgggcg gttgggcctc
      181 actgcctgga ccatcgacgg ctggatcgcc gagcgggccc gtatcgtcag acagttcgtg
      241 ccacccgatc cggccgcacc cgactcgctg agttggggcg atgctggcgt gctggaacgg
      301 aggttgagca ctgagttcac cgacgtgcgg atcgagcgcc ggtcactgcc ctggcatttc
      361 gactccggcg cggcactgac cgagttcctc accgctcatt cgccggcgca cgtcgccgcc
      421 atgcaggcgg ccggtgaccg ggccgatgag atggtcgccg ccatcgaaag gcacgcctca
      481 cccgatggcg gcccggtacg gatcgaggcc gagtacctgc tggcgctagc ccactcgtga
      541 gtgcagcagg ctgggaatgt cctgctgcgc cactggcggg gcggaggcgg tgccacgcgc
      601 ggaatccgcg ccggtgttac caccactgca cctgcgcgga gttgtcgata cccgccacgg
      661 caacggcggt gccagtccgg cccatcaagg gcaccagact ggctagcgcg caacccagcg
      721 ccgaatcggg ctgcgcggcc atgcgcacca gcggcccgga ctgtcgcaac gccagtactg
      781 actcttcaga ccgggaggtc ggtatctctc agtaacccgt atcgtgcgca actgtgcgag
      841 tcctcgtcat cggtgccggc gcgcgggaac atgcactggt attggccttg tccagggacc
      901 cgtcggtcac ggcgctggcc tgcgcccccg gcaatgccgg cactgccgcg ctagcgcaac
      961 agcacggtgt cgatgtcacc gatccggcgg cggtcgcgca actggcgacg tcttggccag
     1021 ctgacttggt ggtgatcgga cccgaggctc cactggtggc cggcgcggcc gacgcggtgc
     1081 gggcagccgg catcgcgtgc ttcggcccgt cggccggcgc ggcgcagatc gagggatcca
     1141 aggcgttcgc gaaggacgtg atggcggtcg ccggcgtgcc cactgcgcgc agtgagctgg
     1201 tcaacaatcc ggcccagctc gacgcggcgt tgagtcgttt ccgaccaccg tgggttgtca
     1261 aggacgaccg gctggccgcg ggaaaaggcg ttgtggtcac cgaggacgtg gaagtagcca
     1321 gggtgcatgc gctgaccctg ctcgatggcg gtcacccagt gctgctggag tcgtatctgg
     1381 agggaccgga ggtctcactg ttctgcctgg tcgatgccag tgccgtggta ccactactgc
     1441 cggctcagga cttcaagcgg gtcggcgaag ccgacgtggg cccgaacacc ggcgggatgg
     1501 gcgcctatgc cccactgccc tgggcgccgg aatcgttggc tactgatgtc accgaacaga
     1561 tcgtgcagcc ggtggtcgat gagttggctc ggcgcggtgc cggattctcc gggttgctct
     1621 atgccggcct ggccctcaca gcgcagggcg cgcaggtcat cgagttcaac tgccgcttcg
     1681 gtgacccgga aacccaggcc gtgc
//
LOCUS       DATADN010000848         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_246146, whole genome shotgun sequence.
ACCESSION   DATADN010000848 DATADN010000000
VERSION     DATADN010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246146"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..2044)
                     /locus_tag="VHH53_11010"
     CDS             complement(101..2044)
                     /locus_tag="VHH53_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2290696.1"
                     /translation="MSGILADGGFLQPADAMDFVETILEASTEYSIIGKGVDGTILLW
                     NEGARRLYGYEPEEIVGKANADLLHTPEAIEAGLPQTMRQAALAFGKWEGTVTRVRRD
                     GSRFTARVVITPRRNRHGEWIGFLLISKDISEEIRLDSTPDAMVIVDQDGRIAVVNRQ
                     TEQLFGYQREELLGQPIEILVPQRFHAAHRRHRSGYLAEPEVRPMGAGMELYGRRKDN
                     SEFPVEISLSPMATPQGTLVSAAVRDITARKQAEEALAQARLAAERANQAKSEYVSRM
                     SHELRTPLNAILGFTQLLELDGLRDDQRESVGYILSGARHLLGLINEVLDIAAIESGR
                     LSLTLEPVDVADLLTEAVSLIRPLADQQGIVLVGQPQAANRHVLSDPQRLKQILLNLL
                     SNAVKYNRNGGSVQLDCEQVAGGWLRIKVTDTGPGIPPESIERLFVPFDRLGSERTSI
                     EGTGLGLPLTKGLAEAMGGTLGVASVVGQGSAFWVELPLTQTASERPTAAEAPPAHAQ
                     PERQHPAVTVLYIEDNLSNLQLVERLLSRRPGVTLISAMRPQLGLELAGEHHPDLILL
                     DLDLPDMPGQEVLRRLRADSNTAHVPIVILSADARPALIARLLEEGARAFLTKPLDIK
                     ELLALLDTITAEQHQAGASAAGS"
     gene            complement(2410..>2675)
                     /locus_tag="VHH53_11015"
     CDS             complement(2410..>2675)
                     /locus_tag="VHH53_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290697.1"
                     /translation="LVRKLTLSPEEVGADDLRATLDAGVSEDGVVDVVYVCFAFNLID
                     RVADALGFDLLDQEGYRQGAQVLLRFGYKLPAPLRLLARNPAW"
BASE COUNT          406 a          878 c          932 g          459 t
ORIGIN      
        1 cagcaagcag caccaggccc gttgcagtga tgaccgtcga gccggcgatg ggcgcgcggg
       61 gctgcgcccg agcatggttg agttggggtg gtgcctgcgc ctaggagccg gcggccgatg
      121 cccctgcttg gtgttgttcg gctgtgatgg tgtccagcag ggcgagcagt tccttgatgt
      181 ccagcggctt ggtcaggaag gcccgtgcgc cttcctccag cagccgggcg atcagggcgg
      241 gcctggcgtc ggcgctgagg atcacgatcg ggacgtgggc ggtgttggag tcggcgcgca
      301 gtcggcggag gacctcctgg ccgggcatgt cgggaagatc caggtcgagg aggatcaggt
      361 cggggtggtg ctcgccagcg agctccaggc cgagctgggg ccgcatggct gagatcagcg
      421 tgacgcccgg ccggcggctc aggaggcgct caaccagctg caggttggac aagttgtcct
      481 cgatgtagag caccgtcacg gccgggtgtt gccgctcggg ttgagcatgt gctggcggcg
      541 cctcggcggc agtggggcgc tcgctggcgg tttgggtgag ggggagctcg acccagaagg
      601 cgctgccctg gccgacgaca ctggccaccc ccagggtgcc gcccatcgcc tcggccagac
      661 ccttggttag gggcagcccg aggccggtgc cctcgatcga ggtccgttcg ctcccgaggc
      721 ggtcgaaggg tacgaacagc cgctcgatcg actccggtgg gatgccaggt ccggtgtcgg
      781 tgaccttgat ccgcagccac ccgcctgcca cctgctcgca gtcgagctgc acgctgccgc
      841 cgttgcggtt gtacttgacc gcgttggaca gcaagttgag caggatctgc ttgagccgct
      901 gagggtcgct caggacgtga cggttggccg cctgtggctg gccgaccagc acgatgccct
      961 gctggtcggc cagcggccgg atcagactga cggcctcggt gagcaggtcg gccacatcga
     1021 ctggctccag cgtcagcgag agccggccgg actcgatggc ggcgatgtcc aggacctcgt
     1081 tgatcagccc gagcaggtgc cgcgcccccg acaggatgta gccgacgctc tcgcgctggt
     1141 cgtctcggag gccgtccagc tccaagagct gggtgaagcc caggatggcg ttcagcgggg
     1201 tccgcagttc atggctcatg cgcgacacgt actcactctt ggcctggttg gcccgctcgg
     1261 cagccagcct ggcctgcgcc agtgcctcct cggcctgctt gcgggcggtg atgtcacgca
     1321 ccgcggccga caccagggtg ccctgcgggg tcgccatggg gctgaggctg atctcaacgg
     1381 ggaactcgct gttgtccttg cgccgcccgt acaactccat cccggcgccc atgggccgca
     1441 cctcgggctc ggccagataa ccgctgcggt ggcggcggtg ggcggcgtgg aaccgttggg
     1501 gaaccaggat ctcgatcggt tggccgagga gctcctcacg ctgatagccg aacagctgct
     1561 cggtctgccg gttgaccacg gcgatacgcc cgtcctggtc gacgatcacc atggcgtccg
     1621 gggtcgagtc cagccggatc tcctcggaga tgtccttgga gatcagcagg aatccaatcc
     1681 actccccatg ccggttccgt ctgggcgtga tgacgacccg tgccgtgaag cgactcccgt
     1741 cgcggcgcac ccgcgtcacg gtcccttccc acttgccgaa ggcgagagcc gcctggcgca
     1801 tcgtctgggg tagtcctgct tcgatggcct ctggggtgtg gagcaggtcg gcgttggcct
     1861 tgccgacgat ctcctcgggc tcatagccat acagccgacg cgcgccttcg ttccacagca
     1921 ggatggtgcc gtcgacgccc ttgccgatga tcgagtattc ggttgatgcc tcaaggatgg
     1981 tctcgacgaa gtccatcgca tcggctggct gcagaaaccc gccgtctgcc aggatgccgc
     2041 tcatcccact cccatcctgt cgagcgtcat ggacaaacgg aacgagcccg cagtcaggag
     2101 gtcggcatac ccgatcgagg gcttgagatc ggccgtcacg atgcagtggc cccgagccaa
     2161 caccccgtgt cacctggaaa ggaactgcgc gaggcgagcg ccgatctggc cggcactcca
     2221 gctgtaaccg acatggacaa gagtcggcta gggcctgatg gcccgccatc gtggcggagt
     2281 cggactgaat cgtttcaacc catcgcctac ctacgtccca ctcggcgagg ctgcctaccg
     2341 ggtccgggcc ttcatacgcc agccgagctg ccgctgtctg gcggctcgtt cacgttctgg
     2401 agtccgggcc taccaggccg ggttccgtgc caatagccgg aggggtgccg ggagcttgta
     2461 cccgaacctg agcaggacct gtgccccctg ccggtacccc tcctggtcca agaggtcgaa
     2521 gccgagcgcg tcggccacgc ggtcgatcag gttgaacgca aaacagacat agaccacgtc
     2581 gaccacgccg tcctcgctca cgccggcatc cagggtcgcc cggaggtcgt cggcgccgac
     2641 ctcctcaggc gacagcgtga gcttgcggac cagcc
//
LOCUS       DATADN010000849         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_561550, whole genome shotgun sequence.
ACCESSION   DATADN010000849 DATADN010000000
VERSION     DATADN010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561550"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1167)
                     /locus_tag="VHH53_11020"
                     /pseudo
     CDS             complement(134..1167)
                     /locus_tag="VHH53_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606173.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          236 a          466 c          546 g          289 t
ORIGIN      
        1 gaatcggaac tgggtgacgc gatcgccgac atccatcacc aagttgtgga tctgatgccc
       61 ctatagatgt caagccccta tagatgtcaa gccgctgacg gctcggcttg gggtggggag
      121 gtgatgatct gatgatctcc cgggcgaggt agcgctttaa gcagcggatg gcttcgcggc
      181 gggttttgcc ttcggtgatg cggcgggtga ggtagttgcg ggtgcgggcg tcgcagcgca
      241 gccggcagat ggcgatgcgg tagagcgcgg aattggcttg gcggtcgccg ccgcggttga
      301 gccggcgacg gctggtcttg cctgaggatg cttctagggg gctgacgccg cacagcgcgg
      361 cgaaggaggc ttcgttgcgc aggcggtcgg ggttgtcacc ggcggcgatg agcagcgctg
      421 cggcgttgtc gggaccgacg ccgcggcggg tcagcaacgt cgggcagtgc tcggtgatcg
      481 tggtggtgat gtggtggaca aggtcgtgga tttcctgggt gagttcaagg attcaccgcg
      541 ccagcaagtg cagggtgtag agcgccgcgc tggtgacatc ttggggcgtg gtggtctcga
      601 gctgcgcgca acgacggatg agcatggtgt tggtcaggcc cgacagcgcc tcgcgcaggg
      661 ctggatcggc cgccacaagc acggctttga gttggttgat ggcctgggtg cgggccttga
      721 tagcggagtc cttggccaac ttcaacatcc gcagcatctc caccggtccg tcaccggcct
      781 tggcgctgcc gctggcccgg ccgctcagga ccgcccgggc ggcggcttcg gcgtcgagag
      841 tgtcggtctt gccctgtcgg cggcgggtgg ccttgtcggt tgcgttgacc tcgatcacct
      901 cgaccccggc cgcccgcagg tgccgggtca gcgctgcacc gtaggatccg gtgcctccac
      961 tccggcccgc cgcagcatcc cgaatgtggc cacccagccc actaacgcct gataacccgc
     1021 cgcggtggtc gggaactttg cggtgcccac cagcaccccc aacgcagtaa tgaccgcagc
     1081 gacgtgcccg tcgtggtggg tatccacccc gagtattacc tcatcggcgg tgctgtcgtc
     1141 ctggtcgtct gacaccagtt gaggcatgct gggcatggtc atttgtctcc tgatcgccac
     1201 ggaatggatg gccgtcgccg gaccggtgtg ggcggtcaga actgtgacgg tgccctgttg
     1261 atggcaaggc ccctatcggg acacgcccac ccagtccggc ggcagcaagc accaccccaa
     1321 gccacggccg acagatcaga cccaaggcat gtcagccagt tgcgtcgcgg gtcagaccat
     1381 gatctgggat ggcacgccgg cgatcctcac aaccagatcc ggacctggga cggaaccacg
     1441 ccgatcctca cagttgcgtt gtctgattgc agtcgccgtg gagtcgcttg tcggtctcgg
     1501 gtcacgctgc ttgagcgagc tcatcgacgg ccgcagc
//
LOCUS       DATADN010000850         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1017931, whole genome shotgun sequence.
ACCESSION   DATADN010000850 DATADN010000000
VERSION     DATADN010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017931"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VHH53_11025"
     CDS             <1..514
                     /locus_tag="VHH53_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEX2290698.1"
                     /translation="RRLAAAAGTSTSAVYALFGGKAGILRGLFIEAFTRFASHLDTVT
                     PSDDPLADLLALGHAYRTSALADPHLYAVMFGSPVPGFEPAPEDYAHAEATFIPLLDT
                     VRRAVAAGLLREADPYLIATALWGNVHGLVSLELGCAMPRQAGAPAAVFEAAIRANLN
                     GWRAEGTAHS"
     gene            570..1796
                     /locus_tag="VHH53_11030"
     CDS             570..1796
                     /locus_tag="VHH53_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HEX2290699.1"
                     /translation="MAADIVPIELSLTAGDMVTLWAPRWREDGEEWEAFLGDGEDLFA
                     FPDVAALVGFVRTTPHHDLVDHPAWSLVSRLPAGNLQPAESQRYDLVGVPELVAQPPD
                     TWVLDELAEVMSIVRSLAEVCGLDVVHEVLDSTPEFGMLEGGMLAFGGREGERCWDSL
                     CAVIAQRWDDVLDTVDGVVRTPDVDAAALAMAQTELTQVEAATDKTNKAAMDKLAAEV
                     PAAPHPALTFWAEVGIDPIRIISDLGDHYTLRCYLDDAPVFLGRNGRIDAFSSPRSLS
                     RYLAGNPKGHDLDEVSTWPQVTKQAHAGELEVAVDDGNSYVLAGLADDLAGGPETVDP
                     GQLELAVELITDAEQWAGSDAADKALAGSEPLGWLVSFLLRPDPTRLAPSPPFDAEVA
                     AWQALVGAFEDRLNVR"
     gene            1897..2898
                     /locus_tag="VHH53_11035"
     CDS             1897..2898
                     /locus_tag="VHH53_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA containing protein"
                     /protein_id="HEX2290700.1"
                     /translation="MSNYSNSPATQSFDRMRSMLVRAAEIRDSEQQQIFDALDEIHAR
                     LAPLESLGSVRKRLSEMPDRTEVSVLAERVDEVLSRLESQDNALTALSRAVESIGDRL
                     AKPFAELGERLDGVAGRFEGFSGRMDSLEERIAGLHKRIEDLDLHLDRQDACLDELPA
                     GVHQPVRDRIDAAELGLRGRLDEVDKSMSERLDGVQQGAGEAAGSLSAALESVTQRLD
                     TLSARLDSVDEGVGGRISSVTERLGKLDGALAYRPDADSMAGLVRQANEESEVRYTAQ
                     LDEAMATFAEIIMGGTPTPLNTAPPPPPRPAQRRVRNGAAKTARSKEDMDTEAVATS"
     gene            2936..>3888
                     /locus_tag="VHH53_11040"
     CDS             2936..>3888
                     /locus_tag="VHH53_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2290701.1"
                     /translation="MIRSRAVVVLIAALSATILLGAVPGTAQLAATTSAAGGCPQQIA
                     PPPPVDLSEVPAPGTPAPPPMSVPNRPVGGPLMGVCGLVLPVNAPLPPAGISAASWLV
                     ADADSGEVLAARSPHARHRPASTIKLLTALLAAQQLPLNRVVVATQDDANQEGSKAGL
                     GPGGRYTVRQLLTGLLLVSGNDTAHALAGQLGGVTKTVEKMNELAAGLGALDTRTATP
                     SGLDGPGMTTSAYDLALVFREVLNRPLLTELLGTRQVSFPGFGERPGFVLSNDNKLLR
                     NYPGALGGKTGFTDDARHTQVDAAQRGGRRLIVVLLRGEQRP"
BASE COUNT          618 a         1332 c         1315 g          623 t
ORIGIN      
        1 gcgccgactc gccgctgcgg ccggcacctc gacgagcgcg gtgtacgcac tgttcggagg
       61 gaaggccggg atccttcggg gtctgttcat cgaggcgttt accagatttg catcccacct
      121 ggacacggtg accccttcgg atgacccgct ggccgacctc ctcgccctcg gccacgccta
      181 ccggaccagc gcactggccg acccacacct gtacgcggtg atgttcggtt cgccggtgcc
      241 cggctttgag ccggctccgg aggactatgc ccacgccgag gccaccttca tcccgctgct
      301 cgacacggtg cgcagggctg ttgcggcggg cctgctgcgc gaggccgacc catacctgat
      361 cgccactgcg ctgtggggca acgtgcacgg cctggtgtca ctggagctgg gttgcgcgat
      421 gccacgtcaa gccggggcac cggctgcggt gttcgaggcg gcgatccgcg ccaacctcaa
      481 cggctggcgg gccgaaggca ctgcgcactc gtgagtgcga ttcggtgctg gaacactgtt
      541 acacactcac gcgcactacg ctgccgcgca tggctgcgga tatcgtgccg atcgagctgt
      601 ccctgaccgc cggggacatg gtgacgctgt gggcccctcg ctggcgtgag gatggtgagg
      661 aatgggaggc gtttctcggc gacggcgagg atctcttcgc cttccccgac gtcgcggcgc
      721 tggtcggttt cgtgcgtacc acgccgcacc acgacctcgt cgatcacccg gcctggtcgc
      781 tggtttcgcg gttgcccgcg gggaacctgc agcccgcgga gtcccaacgt tacgacctgg
      841 tcggagtgcc cgaactcgtc gcgcagccac cggacacctg ggtgctggat gaactcgccg
      901 aggtgatgtc gatcgtacgt tcactggccg aggtatgcgg cctggacgtg gtgcacgagg
      961 tgctcgactc caccccggag ttcggcatgc ttgagggtgg catgctggct ttcggcggcc
     1021 gggagggtga gcggtgctgg gattcgctgt gcgccgtgat tgcacagcgc tgggacgacg
     1081 tgctcgatac ggtcgacggc gtggtccgca ctcccgacgt cgatgcggcg gcgctggcca
     1141 tggcgcagac cgagctcacc caggttgagg cggccaccga caaaacaaac aaagcggcta
     1201 tggacaagct ggcagccgaa gtcccagccg cgccacaccc tgcgttgacg ttctgggcag
     1261 aggtcggcat cgacccgatc cggatcatca gtgatctggg cgatcactac acgctgcgct
     1321 gctacctcga cgatgcaccg gtcttcctcg gccgcaacgg gcgtatcgac gccttctcct
     1381 cgccgcgctc gctgtcccgg tacctcgccg gcaaccccaa gggccacgac ctcgacgagg
     1441 tgtccacctg gccgcaggtg accaaacagg cgcacgccgg cgagctcgag gtcgcagtcg
     1501 atgacggtaa cagctacgtg ctcgccggcc tggccgacga tctcgccggg ggtccggaaa
     1561 ccgtcgaccc gggccagctt gaactagccg tggaattgat caccgatgcc gagcaatggg
     1621 ctggatcgga cgccgcggat aaggcgctgg ccggttctga accgctgggc tggttggtct
     1681 cgttcctgct acggccagac ccgacccggc ttgcacccag ccctcccttc gacgccgaag
     1741 tggctgcctg gcaggccctg gtcggggctt tcgaggaccg gctgaacgtc cgttgagctg
     1801 cgtcacgcgt cgcccgattg ccgcaacggc tcccgagatg gctaagatgc gcgcccaacc
     1861 gtgtcggcgc attctcctca ccgaaagccc acgcctgtga gcaattacag caacagcccc
     1921 gcaacgcagt ccttcgatcg gatgcgcagc atgctcgtgc gtgccgccga gatccgtgac
     1981 agcgagcagc agcagatctt cgacgcgctg gacgagatcc acgcccggct ggctcctctg
     2041 gagtcgctgg gttcggtgcg caagcggctc tcggaaatgc ctgaccgcac cgaggtcagc
     2101 gtgctggccg aacgggtcga tgaggtgctg tcgagactgg aatctcagga caacgccctc
     2161 accgcgttgt cccgcgctgt cgagtcgatc ggggacaggc tggccaaacc cttcgccgag
     2221 cttggcgagc ggctcgatgg ggtggcgggc cggttcgaag gtttctccgg ccggatggac
     2281 agcctcgagg agcggatcgc tggactgcac aagcggatcg aggacctgga cctccacctc
     2341 gaccggcagg atgcctgcct tgatgagctg cccgccggcg tgcaccagcc ggtgcgggac
     2401 cggatcgatg ccgccgagtt gggtctacgg ggtcggctgg atgaggttga caagagcatg
     2461 agcgaacgcc ttgacggggt gcagcagggt gcgggcgagg ccgccgggtc actgtccgcg
     2521 gcgctggagt ccgtcaccca gcggctggat accttgtcgg cccggctgga ctcggtggac
     2581 gagggagtcg gtggccggat cagctccgtc accgaacgtc tgggaaagct ggacggcgcg
     2641 ctggcatacc ggcccgacgc cgactcgatg gccggcctgg tccggcaggc gaacgaagag
     2701 tccgaggtgc gctacaccgc ccaactcgac gaggcaatgg ccaccttcgc tgagatcatc
     2761 atgggtggta cgccgactcc gttgaacacc gcaccaccgc cgcctcctcg accggcccag
     2821 cgccgggtcc gcaacggcgc cgccaagacg gccagatcca aggaggacat ggacaccgaa
     2881 gccgtggcga cgtcctgacg gcagcgcacc cgccacctga cagccgcccg tgcgcatgat
     2941 ccggtcccgg gcggtggtgg tcctgatcgc tgccctcagc gcaacgatcc tcctcggggc
     3001 agtacccggg accgcgcaac tcgccgcaac cacctcggct gccggggggt gcccacagca
     3061 gatcgcccca cccccgccgg tcgacctctc cgaggtcccc gctccgggca ccccggcgcc
     3121 accaccgatg tcggttccga accgtccagt gggcggccct ttgatgggcg tgtgcggttt
     3181 ggtgttgccg gtcaacgcgc cccttccgcc ggcaggaatt agcgcggcgt cctggttggt
     3241 ggccgacgcc gactccggag aggtgctcgc cgccaggagc ccacatgctc gccaccgccc
     3301 ggcatcaacg atcaaactgc tgaccgccct actggctgcc cagcagctcc cgctcaaccg
     3361 ggtcgtcgtc gccacccagg acgatgccaa ccaggagggc agcaaggcgg gactgggacc
     3421 gggcggccgc tacaccgtcc gccagctgct caccggcctg ctactggtgt caggtaatga
     3481 caccgcacac gccctggccg ggcagctggg tggggtcact aagaccgtcg agaagatgaa
     3541 cgagctcgca gccgggctgg gcgcgctcga tacccgaacc gccacgccat ctgggctgga
     3601 cggtccgggc atgaccacct cggcctacga cctggcgctg gtgtttcggg aggtgctcaa
     3661 ccgcccgctg ctcaccgagt tgctgggcac ccggcaggtg agcttccctg gcttcggcga
     3721 gcggccaggt ttcgtgctta gcaatgacaa caaactgctc aggaactatc ccggcgcgct
     3781 gggtggcaag accgggttca ccgatgacgc ccggcacacc caggtcgacg ccgcacagcg
     3841 cggtgggcgg cgcctgatcg tggtactgct gcggggcgag cagcgacc
//
LOCUS       DATADN010000851         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_89686, whole genome shotgun sequence.
ACCESSION   DATADN010000851 DATADN010000000
VERSION     DATADN010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89686"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VHH53_11045"
     CDS             <1..692
                     /locus_tag="VHH53_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290702.1"
                     /translation="GRLPTPVQPGAQPRPDCLHGSMALGLATGSGGAAGSGNQLALIE
                     QCPGERVRLTVQRSTPKQPDQPEVDFSTVLSGSQARVVALSRDRVAVAMPDPARLVVL
                     DKQGKAVAEHPLAVPDSDLRGNPSGGVVEATVTPTAMYWFTSSATVALDIVDLRPRWT
                     RAGSLGPGTEVAGRVLLPVPGALAVVDTTTGQQVGVLPVNRGDYHGPVGTASSGGVVL
                     ERRGGTLVALR"
     gene            700..1575
                     /locus_tag="VHH53_11050"
     CDS             700..1575
                     /locus_tag="VHH53_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2290703.1"
                     /translation="MSPAQITPHGATRVEVRVGSTTLAALRAEPADPAGAVAVLLPGY
                     TGSKEDFAALLDPLRGAGLSVLAVDLPGQYESSGPDREDDYLPDPLGRTLATLVTDLA
                     CGGRQRVLLLGHSYGGLVSRSAVLAGAPVTGLTLLGSGPGALPSGQRRALIDATEPVL
                     RAEGIEVVQRLLEIRDGIAEPPELAALLRARFLSSASAGLLGMATGLRTEPDRVTELA
                     TVLRTGAIPCLVAFGEFDDAWPVPVQREMAVRLGAEVAVVPGAAHSPATENPGALLDA
                     LLPIWRRWLTATEAS"
     gene            complement(1608..1910)
                     /locus_tag="VHH53_11055"
     CDS             complement(1608..1910)
                     /locus_tag="VHH53_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290704.1"
                     /translation="MGAARTVTTGLDVRALRGLSGVLAGGLVALAVAVCAAQWLAGTS
                     GRPGPGTATVAWHVAAALATVALQLAADRLRGQVAVLASGSVLMIAAGVLWFGWYS"
BASE COUNT          294 a          688 c          706 g          290 t
ORIGIN      
        1 acgggcggct gccgaccccg gtgcagccgg gtgcccagcc gcgaccggac tgcctccacg
       61 gatcgatggc gctgggcttg gcaaccggct ctgggggtgc tgccgggtcc gggaaccagc
      121 tggctttgat cgagcagtgt ccgggcgagc gcgtgcgact caccgtgcag cggtccactc
      181 ccaagcaacc cgaccagccg gaggtcgact tcagcaccgt gctgagcggc agccaagctc
      241 gagtcgtcgc gctgagccgg gatcgggtcg ccgtggccat gcccgatccg gcccggctag
      301 tggtgctcga caagcagggc aaggcggtag ccgagcatcc gcttgcggtg ccggactccg
      361 atcttcgggg caatccatcc ggtggcgtgg tcgaggccac cgtgaccccg acggcgatgt
      421 actggttcac cagctccgca actgtcgcgt tggacatcgt cgacctgcga ccacggtgga
      481 cccgagccgg atcgctggga ccgggcacgg aggttgctgg acgggtgctg ttgcccgtcc
      541 ccggcgctct cgccgttgtc gacacgacca ccggccagca ggtcggggtg ctgccggtga
      601 accgagggga ctaccacggc ccggtaggta ccgccagctc cgggggcgtt gtgctcgagc
      661 gccgcggcgg caccctggtt gcgctgcgtt agcggactcg tgagccctgc acagatcacc
      721 ccgcacggtg ccacccgggt cgaagtacgg gttggcagca caacgctggc cgcgctacgg
      781 gccgaacccg cggatcccgc cggtgccgtc gcggtgctgc tgcctgggta caccggttca
      841 aaagaggatt tcgcggcgct gctcgacccg ttgcgaggcg ccgggctgtc cgtactggcc
      901 gtggacctgc cagggcagta cgagtccagc ggacccgacc gcgaggacga ctacctcccc
      961 gacccgctgg gccggacgct ggcgacactg gtgacggacc tggcctgcgg tggcaggcag
     1021 cgagtgctgc tgctgggaca ttcctacggc ggattggtgt cccgatccgc cgtgctcgcc
     1081 ggagccccgg tgaccggcct gaccttgctg gggtcgggtc cgggcgcgtt gccttcaggg
     1141 caacgccgcg cgttgatcga cgccaccgag cccgtcctgc gcgccgaggg catcgaagtc
     1201 gtccagcggc tgctcgagat cagggacggg atcgccgagc cgcccgaact ggccgcactg
     1261 ttgcgggcac gtttcctcag ttcggcgagc gctggcctgc tcggcatggc gaccggcctg
     1321 cgcaccgaac ccgaccgggt caccgagctg gccaccgtcc tgcgcaccgg tgccatcccg
     1381 tgtctggtgg ctttcggcga gttcgacgac gcgtggccgg taccggtgca gcgcgagatg
     1441 gcggttcgac tgggcgccga agtcgcggta gtgcccggtg ccgctcattc gccggctacc
     1501 gagaacccgg gtgcgctact cgacgcgctg ctgccgatct ggcgacgctg gctgacggct
     1561 accgaagcct cgtgagtgcg taacagtgtt atagcactca atgccactca cgagtaccag
     1621 ccgaaccaga gcactccggc tgcgatcatc aggacggatc cggaagccag caccgcgacc
     1681 tggccccgga ggcgatctgc agccagctgc agcgccactg tggccagcgc ggcggccacg
     1741 tgccaggcaa cggtggccgt gcccgggccg gggcggcctg acgtaccggc tagccactgc
     1801 gctgcgcaga ccgcgacagc aagcgcaacc aagccgcctg ccaatacacc ggacagcccg
     1861 cgcagagcgc gtacatcgag cccggtggtc acggtgcgag cagcgcccac ggctggaggt
     1921 ccggtgcact ggcgcggccc tcggggcaat ggcgccggaa atcgcaccat gagcaggc
//
LOCUS       DATADN010000852         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_246909, whole genome shotgun sequence.
ACCESSION   DATADN010000852 DATADN010000000
VERSION     DATADN010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246909"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..651)
                     /locus_tag="VHH53_11060"
     CDS             complement(94..651)
                     /locus_tag="VHH53_11060"
                     /inference="COORDINATES: protein motif:HMM:NF023502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEX2290705.1"
                     /translation="MRTASVRRLIAGMVIVLAALSVLAGCTRTVEGTAAKSGSGGAPR
                     NEDSERQYPNLLKECEVLTEDILAETVGADPLDIQSTFVGAVCRWQAANPSGLVDITR
                     FWFEMGDLDHEREVAQRLNYQIEDRRVAGIQSIVMRPNDPNGACGVASDAAGVVGWWV
                     NPQSPGIDACGMALKLMELTLATRA"
     gene            complement(648..1205)
                     /locus_tag="VHH53_11065"
     CDS             complement(648..1205)
                     /locus_tag="VHH53_11065"
                     /inference="COORDINATES: protein motif:HMM:NF023502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3558 domain-containing protein"
                     /protein_id="HEX2290706.1"
                     /translation="MTHRTRMTRCLAVAAAAVIPLVAACSDSEPAGPQVPEDSPTQTE
                     NVAHGPFFPECGGISDQEIIKQTRVPGLVNTAKTSVGCQWLAGGSILGPHFSFTWFRG
                     SPIGRERKTMELSRTSVEDINIEGHDGFIGYSEDPTNGVNLCEIGIQFDDDFIEWSIS
                     YAAKPFPDPCEVAKELTRQSIVNSE"
     gene            1247..>1673
                     /locus_tag="VHH53_11070"
     CDS             1247..>1673
                     /locus_tag="VHH53_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX2290707.1"
                     /translation="MPVDLLIHPMCDSNGMLWALLRQYARPYRAPLAAVATLQLVSTL
                     ASLYLPTVNAAIIDDGVTTGNLRRIVELGAVMLAVTALQVLCAVGAVYFGSRAGMGVG
                     RDLRAALFAHVTGFSAEDAARFGAPSLLTRTTNDVQQIQL"
BASE COUNT          251 a          578 c          556 g          288 t
ORIGIN      
        1 cccgacctca gccatcgcgg tgctgcgcac cgagcggccg tgggatcagc tggcgctcga
       61 cggcgccgcg ctggtcacct tccacgtgcc gcgttaggcg cgggtggcga gcgtcaactc
      121 catcagcttc agcgccatcc cgcaggcgtc gatcccgggc gactgcggat tgacccacca
      181 gcccacgaca ccggcagcgt cactggccac accgcacgcg ccgttcggat cgttgggccg
      241 catcacgatc gactggatgc cggccaccct gcggtcctcg atctggtagt tcagccgctg
      301 ggcgacttca cgttcgtggt cgagatcgcc catctcgaac cagaacctgg tgatgtcgac
      361 cagccccgac gggttggccg cctgccaccg acacaccgcg ccgacgaacg tgctctggat
      421 gtccagcgga tcggcgccca cggtctcggc gaggatgtcc tcggtcagca cctcgcactc
      481 tttgagcagg ttcgggtact gccgctcgga gtcctcgttg cgcggtgcgc cacccgagcc
      541 cgatttggcc gcggtgccct ccaccgtccg cgtgcaaccg gcgaggacgg acagcgcggc
      601 gagcacgatg accatgcccg cgatcagccg acggacggac gcggttctca ttcggaattc
      661 acaatcgatt ggcgggtcag ttccttggcc acttcgcagg gatccgggaa cggcttcgcg
      721 gcgtagctga tcgaccactc gatgaagtcg tcgtcgaact ggataccgat ctcacacagg
      781 ttgacgccgt tggtcggatc ctcgctgtag ccgatgaacc cgtcgtggcc ctcgatgttg
      841 atgtcctcga cgctggtgcg cgacagctcc atggtcttgc gctcccggcc gatggggctg
      901 ccgcggaacc aggtgaacga gaagtgcggg cccaggatgc tgccgccggc cagccactga
      961 cacccgaccg aggtcttggc ggtgttgacc aaccccggca cccgggtctg cttgatgatc
     1021 tcctggtcgc tgataccgcc gcattcggga aaaaaggggc cgtgcgcgac gttctccgtc
     1081 tgcgtcggac tgtcctccgg cacctggggg ccggccggtt cggaatccga gcatgcggcg
     1141 accagcggga tgaccgccgc ggccgcaacg gccaggcagc gggtcatgcg cgtcctgtgg
     1201 gtcacgccat gcactgtagc ggccgccctc gtggtcaacc accgacatgc cggttgacct
     1261 gctgattcat ccgatgtgcg acagtaacgg gatgctatgg gcgctgctgc gacagtacgc
     1321 gcggccatac cgggcaccgc tcgccgccgt cgccacgctg cagttggtca gcacgctggc
     1381 gtcgctgtac ctgccgaccg tcaacgcggc gatcatcgac gacggcgtca cgacgggcaa
     1441 cctgcgcagg atcgtcgagt tgggcgccgt gatgcttgcg gtcaccgcgc tgcaagtgct
     1501 gtgtgcggtc ggcgcggtgt acttcgggtc gcgcgcgggc atgggcgtgg gccgcgacct
     1561 gcgcgcggcg ctgttcgcac acgtgaccgg cttctcggcc gaggacgccg cccggttcgg
     1621 tgcgccgtcg ctgttgaccc gcactaccaa cgacgtgcag cagatccagc tgc
//
LOCUS       DATADN010000853         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_90237, whole genome shotgun sequence.
ACCESSION   DATADN010000853 DATADN010000000
VERSION     DATADN010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90237"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1546
                     /locus_tag="VHH53_11075"
     CDS             68..1546
                     /locus_tag="VHH53_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2290708.1"
                     /translation="MSTTYGGVGLGMLAAELTTVLGPAAVITDRQRLRTYECDGLAQY
                     RVVPGIVVLPESAEQIAAVVKTCAAAGVPFVARGSGTGLSGGALPHAEGVLIVTSRMR
                     RILEVDPANERAIVEPGVINLDVTRAAAPQGWYFAPDPSSQQICSVGGNVAENSGGAH
                     CLKYGFTAHHVLGAEVVTPDGELVWLGGTTRDVPGYDLLGAFVGSEGTLGIATKIVVR
                     LLREPESVRCLLAAFTGVDEAGAAVSAIIADGVTPAAIEMMDALAIEAAEAAVACGYP
                     EGAGAVLIVELDGPVAEVEHTFAAVHRHCTSAGAFEIRVACDATERARFWKGRKSAFA
                     AVGRISPDYIVQDGVIPRTALPEVLHKIADLSAGTGVRVANVFHAGDGNLHPLVLFDE
                     AKPGEAQAAEEVSGAILDLCVEYGGSITGEHGVGTDKAQYMPRMFTAEDLDTMQRLRC
                     AFDPAGVCNPGKVFPTPRLCGEVPGKRKGVHPLQAAGLAEQF"
     gene            1546..>2753
                     /locus_tag="VHH53_11080"
     CDS             1546..>2753
                     /locus_tag="VHH53_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2290709.1"
                     /translation="MVAPTFDGLRAACAEVAQASTEDTVDGVPARWVAWTRSTEEVSA
                     VLRAAAGLGLRVVARGSGSRLCWGLPPTDLDLIVDTSRMTAVLAHAAGDLVVTTQAGV
                     RMVDLQNQLGVHRQQLALDGSDTLGGTIGGTIATATSGPLRYRFGSVRDLLIGITVVL
                     ADGTVARSGGTVVKNVAGYDLGKLYTGSMGTLGVITEAVFRLHPTPEASRWILAPVAD
                     AARAAAAVAAVRAAQHDPVALEVNRGTPGAPVTVGVAVEGVAKGIDARADAVASLLSG
                     AAGMPATVTKQPPDWWGRPPHPPQGPGATVTCEPSGLADLIATAPGALRGSAGAGVLA
                     VGLDDGDRLSNELAQLRTIASRHGGNAVLRCAPPHRKATLDVWGPVPALGLMRRLKDQ
                     FDPEHRLSPGR"
BASE COUNT          397 a          902 c         1032 g          422 t
ORIGIN      
        1 atccttggca agctgcgaca ctgggcgaag ggccggcgaa ggcgatgccg gaccagggga
       61 gcgggcgatg agtacgacat acggcggcgt tgggttgggg atgttggccg cagagctcac
      121 cactgtgctc gggcctgcgg cagtgatcac cgaccggcaa cggctgcgca cctacgagtg
      181 tgacgggctg gcccaatacc gcgtggtgcc gggcatcgtg gtgctgccgg agtccgccga
      241 gcagattgcc gcggtggtga agacctgcgc cgccgcggga gtgccgtttg tagctcgcgg
      301 ctcgggtacc gggctgtccg gcggggcgct gccgcacgcc gagggtgtgc tgatcgtcac
      361 ctcgcggatg cgtcgcatcc tggaggttga cccggccaac gagcgcgcga tcgtcgagcc
      421 cggcgtgatc aacctggacg tgacgcgggc ggccgcgccg cagggctggt acttcgcccc
      481 cgacccttcc agccagcaga tctgctcggt gggcggcaac gtcgcggaga actccggtgg
      541 cgcgcactgc ctcaagtacg ggttcaccgc tcaccatgtg ctgggtgccg aggtggtcac
      601 cccggacggc gagctggtct ggctcggtgg caccacccgc gacgtgcccg gatatgacct
      661 gctgggtgcg tttgtcggca gtgagggcac cctggggatc gctaccaaga tcgtcgttcg
      721 attgctgcgg gaaccggaat cggtgcgctg cctgctcgct gcgtttaccg gagtggacga
      781 ggcaggcgcc gcggtatcgg cgatcatcgc cgacggcgtc accccggcgg ccatcgagat
      841 gatggacgcg ctggcgatcg aggccgccga ggccgccgtg gcctgcggat atcccgaggg
      901 tgccggtgcg gtgctcatcg tggagctgga cggcccggtc gctgaggtcg agcacacctt
      961 cgccgcggtg caccggcact gcaccagtgc gggtgcgttc gagatccggg tggcctgcga
     1021 cgccaccgag cgcgcgcggt tctggaaggg tcgcaagtcc gcattcgccg cggtgggccg
     1081 gatcagcccc gactacatcg tgcaggacgg ggtgattccc cgaacggcgc tgcctgaggt
     1141 gctgcacaag atcgccgacc tgtcggccgg caccggcgtg cgggtggcca acgtgttcca
     1201 cgccggggat ggcaacctgc acccgctggt gctgttcgac gaggcgaagc cgggggaggc
     1261 acaggccgcg gaggaggtct ccggggcgat cctcgacctg tgtgtcgaat acggcgggtc
     1321 gatcaccggc gagcacggcg tcgggacgga taaggcgcag tacatgccgc ggatgttcac
     1381 cgctgaagac ctcgacacca tgcagcggct gcgctgtgct tttgatcccg ccggggtgtg
     1441 caaccccggc aaggtgttcc cgactccccg gttgtgtggg gaagtgcccg gcaagcgcaa
     1501 aggcgtgcac ccgctgcagg ccgcgggcct ggcggagcag ttctgatggt cgcccccacc
     1561 ttcgacggtt tgcgcgcggc ttgcgcggag gtggcacaag cctccacgga ggacaccgtg
     1621 gacggggtac cggcgcgttg ggtggcctgg acacgcagca ccgaggaggt ctcggcggtg
     1681 ctgcgcgcgg cggccgggct gggtcttcgg gtggtcgccc ggggtagcgg tagccggctg
     1741 tgctggggtc tcccacccac cgacctggac ttgatcgtgg acaccagccg gatgaccgca
     1801 gtgcttgcgc atgcggccgg tgacctggtc gtcaccaccc aggccggcgt gcggatggtc
     1861 gatctgcaga atcagttggg cgtgcaccgg cagcaactcg ccctggacgg tagtgacact
     1921 ctcggaggca ctatcggggg caccatcgcc accgcaacgt cgggtccgct gcgctaccgg
     1981 ttcggctcgg tgcgcgacct tctcatcggc atcaccgtgg tgctcgccga cggcaccgtc
     2041 gcgcgctccg gcgggacagt ggtgaagaac gtcgctggat acgacctggg caagctctac
     2101 accggctcaa tgggcacgct gggtgtcatc actgaagcgg tgttccggtt gcatccgacg
     2161 cccgaggcca gccgctggat tctcgcgccg gtcgctgacg cggctcgggc cgcagccgcg
     2221 gttgccgcgg tccgggcggc gcagcacgac ccggtcgccc tcgaagtgaa ccggggcacg
     2281 ccaggcgctc cggtgaccgt cggggtcgcc gtggagggcg tcgccaaagg catcgatgct
     2341 cgcgccgatg ccgtggcaag cctgctcagc ggcgcagctg gcatgccggc cacggtgacc
     2401 aagcaaccac cggactggtg gggccggccc ccgcacccgc cgcagggacc tggcgcgacg
     2461 gtcacgtgcg agccgagcgg actggccgac ctgatcgcca ccgcacccgg cgccctgcgc
     2521 ggcagcgccg gcgctggggt gctcgccgtt ggcctggatg acggtgaccg gctgagcaat
     2581 gaactggcgc agctgcgcac aatcgccagc cggcacggtg gaaacgcggt gctgcgctgc
     2641 gcacccccgc atcgcaaggc aacgctggac gtgtggggac cagtgcccgc gctggggttg
     2701 atgcgccggc tcaaggatca attcgatccc gagcaccggc tgtcaccggg gcg
//
LOCUS       DATADN010000854         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_90544, whole genome shotgun sequence.
ACCESSION   DATADN010000854 DATADN010000000
VERSION     DATADN010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90544"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHH53_11085"
     CDS             complement(<1..227)
                     /locus_tag="VHH53_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290710.1"
                     /translation="MAEADGPRIHRVCSSREVHLDQVERLLAVVSAEKAGWWRRLRLR
                     FAARRLRRLAGGDGSPTAMRRAVRAARDQRDA"
     gene            835..1878
                     /locus_tag="VHH53_11090"
     CDS             835..1878
                     /locus_tag="VHH53_11090"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2290711.1"
                     /translation="MLVVGIDPHKDSHTAAALDTSTGQMLGEITIAATPRGYRELLEW
                     LECFTACHIAIENARGLGRHLAAWLGGQGFAICDVSTKEVRAQRLARRGGRNKNDTND
                     ALAAAHAAATGSGYRLAGDEHGEIAALLTEEREALNAELTAKRNRLHAYLRVLRPGGA
                     PTGASVEDFANLLKGVKATTSVVAETKRLARNLLAELRRLARALEANQQRMSDLTEQT
                     RTGLTAIDGIAVVSAVAIMGAVGDAGRFPNQDHLAAFAGAAPKQIASGSSDRHRLDRR
                     GDYRLKRAIHLAAMTQARMKNGPGRVYYLKKREEGKTHREALRCLKRQLVKVIWRTLR
                     EDSRRRETLTLAA"
BASE COUNT          417 a          776 c          783 g          315 t
ORIGIN      
        1 gcatcgcgct ggtcgcgagc ggcgcggacg gcccgacgca ttgcggtggg actgccgtcg
       61 ccgcccgcga ggcggcgcaa ccgcctggcg gcgaagcgaa gccgcagccg gcgccaccag
      121 ccggccttct cggcggacac cactgcgagc agtcgttcga cctggtcgag gtggacttcg
      181 cgcgaactgc acacgcgatg aatccgcggg ccgtccgcct cggccatggc gcgcatacct
      241 cgctagttgg cggctttctg gtcgggggtt gcttgcggtc agggcggcag cgacgaagcg
      301 gtggagggtg gggcggggcc cggtggcggt ggccgggccg gtgtccccag gggcggggtg
      361 gggctcggtg gcggtcctgc gggtcaagtt cggcgacatg cccgagcgcg gggtgcgggc
      421 acctgtttcc ttgcagtgca gcaggtgtga cgcggaacgt gagggttctg tactcgtgtg
      481 ggtgatgacg gtgggggttt gcatgcgaga caattgaggt acgtacaggg acagggaatt
      541 cgtacaggtg tcgagtaagg tgcaagcaga ctaagagaag ccgattagtg tgggctgcag
      601 cagatctgct ggcgggtcgc tcctggtggc gttctgtgag gggaccggcc gggctggctc
      661 gatataagcc tgccgcggca tgtggccgtt ggctctccag aggtgtgccg cccggccggt
      721 caccgtctcg gtctggctcg ataagggaat gccgaacggg atgttctggc tggtaatcgg
      781 cttgccgacc gagatgccat cctcaacctc gaatcggcaa aggacgcaat cctcatgcta
      841 gtggtcggca tcgaccccca caaggactcc cacaccgccg ccgcgctcga cacctcgacc
      901 ggccagatgc tcggtgagat caccatcgcc gccactcccc ggggctaccg cgagctgctc
      961 gagtggctcg agtgcttcac cgcctgccac atcgcgatcg agaacgcccg gggcctgggc
     1021 cgccacctcg ccgcctggct cggcggccaa gggttcgcca tctgcgacgt gtccaccaag
     1081 gaagtccgcg cccagcgcct ggcccgccgg ggcgggcgca acaagaacga caccaacgac
     1141 gccctggccg ccgcccacgc ggccgcgacc ggctcgggct accggctggc cggtgacgag
     1201 cacggcgaga tcgccgcgct gctgaccgag gaacgggaag cgctcaatgc cgagctgacg
     1261 gccaaacgca accgcctcca cgcctacctg cgcgtcctgc gtccgggagg cgcaccgacc
     1321 ggcgcgagcg tcgaggactt cgcgaacctg ctcaagggcg tcaaagcgac cacgagtgtc
     1381 gtggccgaga ccaagcggct ggcccgtaac ctcctcgccg agctgcggcg cctggcccgc
     1441 gcgctcgagg cgaaccagca gcgcatgtcc gacctgaccg aacagacccg caccggcctg
     1501 actgccatcg acggcatcgc cgtcgtcagc gcggtcgcga tcatgggcgc tgtcggcgat
     1561 gcgggccgtt tccccaacca ggatcatctc gccgcgttcg ccggcgcggc acccaaacag
     1621 atcgcctcag gcagctcgga ccgccaccgc ctcgaccgca gaggcgacta ccggctcaaa
     1681 cgggcgatcc acctcgccgc gatgacccag gcacgcatga agaacgggcc cggacgcgtc
     1741 tactacctga agaaacgcga ggagggcaag acccaccgcg aagcgctccg ctgtctcaaa
     1801 cgccagctcg tcaaggtcat ctggcgcacc ctccgcgaag actcgcgacg acgggaaacc
     1861 ctcacgctcg ccgcttgaca aacataaggg aacctctggc cgatccgaca aaccgacttg
     1921 tttcgaggca ctcagccccc tctcaggggg ttccagatct catcccagtg ctatcgcccg
     1981 cagctgtccg cgcccccgat gggccgtgat tgggtgggag atttcctcgt tgggatcacc
     2041 ccaaccagaa ggcagtgcct cacagggagg cacacgcgaa ccaggatgcc accagccgcg
     2101 ccagctccgg ccgggaatgg tggacgcccg gcaacgaagc ccaccggcac aagcgagacc
     2161 aacagccaag accaacggcg aaaagccaaa ggcgaaaggc caaggctgag ggctgaaggc
     2221 gaaacgccaa gggccggggg ctgaaggctg ccagtggccg caggctgcca acgaaagcca
     2281 gcgagggaaa c
//
LOCUS       DATADN010000855         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_709143, whole genome shotgun sequence.
ACCESSION   DATADN010000855 DATADN010000000
VERSION     DATADN010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709143"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..311)
                     /locus_tag="VHH53_11095"
     CDS             complement(42..311)
                     /locus_tag="VHH53_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290712.1"
                     /translation="MTAAIESMTGARFVHDQLELRGWAVEIADAVKVKGFAPLACKTD
                     RIDAWVLAELSRRDLVPAIWLPTPGCAPTGSGPAGGCTWSGTAPR"
     gene            667..1980
                     /locus_tag="VHH53_11100"
     CDS             667..1980
                     /locus_tag="VHH53_11100"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2290713.1"
                     /translation="MREFPAPRDAARLEVAVSEATVKRAPLVQVAAAVAPRVPEIVGE
                     LVDTLLRQVGELRGDEVVIGRLRASIESNVSQLLHLMEQSVDLHLVEPPAGALQFAQR
                     LAQRGVPLSALWRAYHLCNARFFVICLHELARHGDTVMDLAEQATVLSELLNTYVDHV
                     CDRIGTTYDVERERWLRQQDAVRTERLSELLAGHVGGTAEVEAALGYRLTGQHLGVVL
                     WDTEAAGDVTDLVRLQRLVTSYADRLGCREQPLVVPRDQSTVWAWLSVPARSAVDHTA
                     VLVQLLGTASTVRAALGDPGAGVPGFVRSHRHAAVAQSVALAASGVTQTVTPYTAVAG
                     LSFLCQDLDRAREWVRETLGVLAVDDETHARLRESVSAFLDTGGSLSAAAERLGCHKN
                     TVHHRIRRAEDELGRDVRDRRVDLELALEACRWLGSAVLAGAPRG"
BASE COUNT          310 a          805 c          800 g          348 t
ORIGIN      
        1 tgggtggccg aaggccagca gggtggcgtg gatgcggttc ttcagcgcgg tgcggtgccg
       61 gaccaggtgc agccgccagc gggcccgctc ccggtcggcg cgcaccctgg ggtgggcagc
      121 cagatcgccg gcaccagatc acggcgcgag agctcggcca gcacccacgc atcgatcctg
      181 tcggtcttgc aggccagcgg ggcaaagccc ttgaccttca ccgcgtcggc gatctccacc
      241 gcccagccgc gcagctccaa ctggtcgtgc acgaaccggg ctccggtcat cgattcgatg
      301 gccgcggtca ccggctgccc gaactcggcg agccgatagg ccagcgacgc gagtcctccg
      361 gcatcggtgc cgcggtggtc accgcgatcg gcgcacccgt ctcgtccatg gcatgcacat
      421 ccagacgagt cctgctcaga tccaacccaa cgtgcaacat gaggaccagg cctccttccg
      481 tcacacctgc cggctctcac cgcaagcgtg atccggggga agacccccgc cttcatggca
      541 ttcagggaca cctctgctca gggacacctc tgctccgccg atcccggagc gcacttcgat
      601 gtctctcgac gagccgaatg cgccggggcc attcgtttat gaccactggt tcaggctcgt
      661 tcgcggatgc gagagttccc tgcgccgcgc gacgcggccc ggctggaggt ggcggtgtcc
      721 gaggcgacgg tgaagcgtgc gccgctggtg caagtcgcgg cggcggtcgc gccgagggtt
      781 cctgagatcg tcggcgaact ggtggacacg ctgctccgcc aggtcggtga gctccgcggg
      841 gacgaagtgg tcattggtcg cctgagggcc agcattgagt ccaacgtgtc gcagcttctg
      901 cacctgatgg agcagtcggt cgacctgcac ctcgtggagc ccccggcggg ggcgctgcag
      961 ttcgcgcagc ggctcgcgca gcgcggtgtg ccgctcagtg cgctctggcg cgcctaccac
     1021 ctgtgcaacg cacggttctt cgtcatctgc ctgcacgaac tggctcggca tggcgacacg
     1081 gtcatggacc tcgcggagca ggcgacagtg ctgagcgagc tgctcaacac ctacgtcgac
     1141 cacgtctgtg accggatcgg gacgacctac gacgtggagc gggagcgctg gctccggcag
     1201 caggatgccg tccgcaccga gcgactctcc gagctgctgg cgggccatgt cggcggcact
     1261 gcggaggtgg aagccgcgct gggctaccgg ctgaccggcc agcacctcgg ggtggtcctc
     1321 tgggacaccg aggcggccgg ggacgttacg gacctcgtgc gcctccaacg tctggtgacc
     1381 agttacgcgg accggctcgg ctgccgggag cagccgctcg tggtgccgcg cgaccagtcg
     1441 acggtgtggg cgtggctgtc ggtgcccgct cgttcggctg tcgaccacac agccgtgctg
     1501 gtgcagctcc tgggtaccgc atcgacggtc agggccgcgc tcggcgatcc cggggccggg
     1561 gtgccgggct tcgtgcgctc gcaccggcat gccgccgttg cgcagtccgt ggcgctcgcg
     1621 gccagcggtg tcacccagac cgtgaccccg tacacggcgg tcgccggcct gagcttcctc
     1681 tgccaggacc tcgaccgtgc ccgggagtgg gtccgggaga cgctcggtgt gctggccgtc
     1741 gacgacgaga cccacgcccg gctgcgggag agcgtcagcg ccttcctgga caccggcggc
     1801 agcctgagcg ccgccgccga gcgtctgggc tgtcacaaga acaccgtgca ccaccgcatc
     1861 cggcgggccg aggacgagct cggccgcgac gtccgtgacc ggcgcgtcga cctcgagctg
     1921 gccttggagg cctgccgctg gctcgggtcg gcggtgctgg ccggcgcacc gaggggctga
     1981 cccgctccgg gccggacctg ttcgcgaacg ccgacggctc gctacccgcg cggccggttg
     2041 tgccgacagc acacccgacg ccggaaagct gcccgttcag cccgatgacc gggcgagcgt
     2101 gaccggcttc acttgtgctc agtcgacgtg gttccggtca cagcccggac gccggtcggc
     2161 agccgcgcag cagcggcccg cggtatgcgc acccgggcgg ctggcggatt ccctgtctct
     2221 ccctgccgag gaggcctcat gtcctctgcc accaccccgg tcc
//
LOCUS       DATADN010000856         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1169301, whole genome shotgun sequence.
ACCESSION   DATADN010000856 DATADN010000000
VERSION     DATADN010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169301"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VHH53_11105"
     CDS             complement(<1..750)
                     /locus_tag="VHH53_11105"
                     /inference="COORDINATES: protein motif:HMM:NF042604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6779 domain-containing protein"
                     /protein_id="HEX2290714.1"
                     /translation="MSGPDERPLPRGCGRVLMLGTVVLAAAAAVVLALGAQDARLLRL
                     GLIAALWAALLGAFAAAQMRREISSDANRVDELRTVYQLELEREVAARREHMLTVERE
                     LREQAEQSERGEIAALRVELATLRANLEKLLGGDRLVERLELRAESARVLPMAAYRHA
                     VGDSSSFDNRCSATAALAATAKVAQVVPPLIPQVNHSLTAAGLSVRVPGGSGSAVFGA
                     QDLQDPLPTSEWPINERNDTAAIPRDDAELCF"
     gene            complement(863..1324)
                     /locus_tag="VHH53_11110"
     CDS             complement(863..1324)
                     /locus_tag="VHH53_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HEX2290715.1"
                     /translation="MTPTRARDLIGAGAVAAVLVNLLLQLSYGDLPTLPTLAGTTLFV
                     FAVAEVVLGTMLRTRIRQRPRTRPVQALTAVKALVLAKASSLAGAVTGGAWLGVLGYV
                     LPLRSELQSAASDTVTASLGAVCAGALVAAGLWLEYCCRTPDDPEDAEPDR"
     gene            complement(1321..>1749)
                     /gene="folK"
                     /locus_tag="VHH53_11115"
     CDS             complement(1321..>1749)
                     /gene="folK"
                     /locus_tag="VHH53_11115"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX2290716.1"
                     /translation="LLGDAVRGVSPVYATAPWGGVTQAEFLNAIVLVDDPDADVPEWL
                     RRGQRCEAAAQRTRDVRWGPRTLDVDVIDVAQTRWNEPELILPHPRAHQRAFVLVPWL
                     DVQPGAVLAGYGTVEQLVAALPASERRSVQRRDDLELGSS"
BASE COUNT          298 a          641 c          562 g          248 t
ORIGIN      
        1 gaagcacagc tcggcatcat cgcgagggat cgcagcggtg tcgttgcgct cgttgatcgg
       61 ccactcggag gtgggcagcg ggtcctgcag gtcctgggct ccgaacactg cactgcctga
      121 accacctgga acccgcaccg acaatccggc ggcagtgagg ctgtgattga cttgggggat
      181 tagtggggga actacctggg ctaccttcgc cgtcgctgcc aacgctgcgg ttgcgctgca
      241 cctgttgtcg aagctggagc tgtcgccgac cgcgtggcgg taggcagcca tcgggagcac
      301 gcgggcggac tcggcccgta gctcgagccg ctccaccagc cgatccccgc ctaacaactt
      361 ctcgaggttc gcgcgcaagg tggcaagctc aacgcgcagt gctgcgatct caccccgctc
      421 ggactgctca gcctgctcgc gcaactcccg ttccacggtc agcatgtgct ctcgccgggc
      481 ggccacctcg cgctccagtt cgagttggta gaccgtccgc aactcgtcga cacgattggc
      541 gtccgagctg atctcgcgcc gcatctgcgc cgctgcgaag gcaccgagca gcgccgccca
      601 cagcgccgcg atcaggccca gcctcagtag gcgggcatcc tgtgccccca gcgcgaggac
      661 caccgctgcc gccgcagcca gcacgacggt tcccagcatc aggacccgac cgcacccacg
      721 cggcaatgga cgctcatcgg ggccggacat ggcgcacacc gtaccgctca agcagtcatc
      781 gaggtgagcg tccggcggtg acaggcagct atcgtgcgat gacaggacgt ccgcagcaag
      841 ctggggtgac atccgatgtc gatcagcggt ccggttcggc gtcctcggga tcatccgggg
      901 tccggcagca gtactccagc catagcccag ccgccaccaa cgcccccgcg cacaccgccc
      961 cgagcgacgc ggtgacggtg tccgaagcag cggactgcaa ttcggagcgc aacggcagca
     1021 catagccgag cacgcccaac caggcgcctc cagtgacggc accggccaac gaggacgcct
     1081 tggccagcac gagcgctttc acggcagtca gtgcctgcac cggtcgggtc ctcggccgct
     1141 gccggatccg cgtccgcagc atcgttccca gcacaacctc ggcgaccgcg aagacgaaca
     1201 gcgtggtgcc ggccagcgtc ggcaacgtgg gcaggtcgcc ataggacagc tgcagcagca
     1261 ggttcaccag taccgccgcg acggcgccag caccgatcag gtcacgtgcc cgagtggggg
     1321 tcacgacgat cccagctcca gatcgtcgcg gcgctgcaca ctgcgcctct cggaggccgg
     1381 cagcgcggcc accaactgct ccaccgtgcc gtaaccggca agcaccgcgc cgggttgcac
     1441 gtccagccag ggcaccagca cgaaggcgcg ctggtgggcc cgcgggtgcg gcagtatgag
     1501 ttccggctca ttccagcggg tctgcgccac gtcgatcaca tccacgtcca gggtgcgcgg
     1561 cccccaccgg acatcgcggg tgcgttgcgc cgcggcctcg caacgctgcc cgcggcgtag
     1621 ccattccggc acgtccgcgt ccggatcgtc caccagcacg atggcgttga gaaactcggc
     1681 ctgcgtgaca ccaccccatg gtgccgttgc gtacaccggt gacacccctc gcaccgcgtc
     1741 gcccagcag
//
LOCUS       DATADN010000857         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1469262, whole genome shotgun sequence.
ACCESSION   DATADN010000857 DATADN010000000
VERSION     DATADN010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469262"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VHH53_11120"
     CDS             complement(<1..1078)
                     /locus_tag="VHH53_11120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2290717.1"
                     /translation="MLWLALATLLAMATWFSASAVVPALSQVWNLDEAGRAWLTMSVQ
                     LGFVVGALASALFNLADRIPARWLFALSAVLAATATALIPIASGLVLALPLRFFTGLA
                     LVGVYPVAMRIVATWTRIDRGLGIGLLVGAITLGNAMPHLLNAIGGADDWRRVLYAAA
                     GLAVLGALIAAVVAREGPYATRIPPFDWRQAGRILRQREMVLVNLGYLGHMWELFAML
                     TWLPIFLAASFRASGHDPAWASLTAFVAISMGAPSSLVAGILADRLGRTTITIAALSI
                     SGSCALLTGWLFGAQPALLVPLCLLWGLAVSPDSAQFSATASELSRPEYIGTALTLQT
                     CLGFLLTLATIRLVPSLEPWAGWGW"
     gene            complement(1200..>1593)
                     /locus_tag="VHH53_11125"
     CDS             complement(1200..>1593)
                     /locus_tag="VHH53_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX2290718.1"
                     /translation="AVDIHVKYAQELPTWKSTMHLYPINGVAGRVARDATAWNYRDAK
                     WGMVIVGVSPDPADNPGMIAWARAYWAALHPYSAGGAYVNMMMDATDEGQDRVRASYG
                     DHYDRLRQIKGKYDPNNLFRVNQNITPA"
BASE COUNT          296 a          502 c          556 g          239 t
ORIGIN      
        1 cccaccccca gccggcccag ggttccaaac tcggcacgag ccggatggtg gccagcgtga
       61 ggagaaagcc gaggcacgtc tgcagggtga gtgccgtccc gatgtactcc ggccgcgaga
      121 gctcgctcgc agtagcggag aactgcgccg agtcggggct caccgcgagc ccccagagca
      181 ggcacagtgg cacgagcaag gcgggttggg ctccgaagag ccagccggtc aggagggcac
      241 agctgccgct gatggacagc gctgcgatcg tgattgtggt gcggcccagc cggtcggcca
      301 ggatcccggc aaccaggctg ctaggggcgc ccatgctaat ggcgacaaac gcggtgaggc
      361 tggcccaggc cgggtcgtgg ccgctcgctc gaaagctggc cgccaggaag atcggtagcc
      421 aggtgagcat ggcgaagagc tcccacatgt ggccgaggta gcccaagttg actaggacca
      481 tttcccgctg tctcaggatt cggccggctt ggcgccagtc gaagggcggg atccgggtcg
      541 cgtacgggcc ttcgcgcgcc acgaccgcgg cgatcaacgc cccaagtacg gccaggccag
      601 ccgcggcgta gagcacccgc cgccagtcat ccgccccgcc gatcgcgttg agcagatgcg
      661 gcatcgcatt gccgagtgtg atagcaccga ccaacaaccc gatccctaag ccgcggtcga
      721 tccgggtcca agtggccacg atccgcatgg cgacggggta gaccccgact agggcgaggc
      781 cagtgaagaa gcgcaacggt agggcgagga ccaggccaga ggcgatggga atcagggctg
      841 tggctgtcgc ggccagcacc gccgagagcg cgaagagcca gcgtgcgggg atgcgatcgg
      901 cgaggttgaa cagcgccgac gcgagggcgc cgacgacgaa accgagttgg accgacattg
      961 tcaaccaggc ccggcccgcc tcgtcgagat tccagacctg cgagagtgcc gggaccacgg
     1021 ccgaggccga gaaccaggtc gccatcgcga gcagcgtggc cagcgccagc cagagcaatg
     1081 tcggccactt cccgagacct gggaccagat actcgcgttg taccaatgtt agttgaggca
     1141 aatcggttgg acgggctaga gcacagccct agcccgttcg caccctttga agcggtggac
     1201 tacgccgggg tgatgttctg attgacgcgg aagaggttgt tcgggtcgta cttgcccttg
     1261 atctggcgca gccggtcgta gtggtcaccg taggaggcac gcacccggtc ctgcccttcg
     1321 tcggtggcat ccatcatcat gttgacgtag gcgccgccag ctgagtacgg gtgcagcgct
     1381 gcccagtagg cgcgcgccca ggcgatcatc cccgggttgt cggccggatc gggactgact
     1441 ccgacgatga ccatgcccca cttagcgtcc cgatagttcc aggcggtagc gtctctcgcc
     1501 acccggccgg cgacgccatt gatcgggtag aggtgcatcg tcgatttcca ggtcggcaat
     1561 tcctgggcgt atttgacgtg gatgtcgacg gct
//
LOCUS       DATADN010000858         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_248007, whole genome shotgun sequence.
ACCESSION   DATADN010000858 DATADN010000000
VERSION     DATADN010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248007"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VHH53_11130"
     CDS             complement(<1..268)
                     /locus_tag="VHH53_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol-binding protein"
                     /protein_id="HEX2290719.1"
                     /translation="MDPRAFAKLIKYASNEQLDAVLDDSTLRRALLDRIFSRMADQFR
                     PENAPQRDSAIHWRLTGGDGDGELYETWITGNHGGSSTPQCVTTS"
     gene            complement(535..1398)
                     /locus_tag="VHH53_11135"
     CDS             complement(535..1398)
                     /locus_tag="VHH53_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX2290720.1"
                     /translation="MEAATEMIELATPPATVPLFTKAALGALPGPRRPPQLPDTALAL
                     PAQIVDRHRLAAYARVCGFPLSDRLPVTYPHVLSFGLVLQLMTGRNFPFPLLGLVHIG
                     NRITVRRVIRADERLTLQVRAIDLRPHDRGSQFTVITQVTADGALVWSERSAYLYRSG
                     HRDKARRSGHAVAQSPPEPTAVWQLAGDVGRRYAAVSGDYNPIHLHRLTARGFGFPQA
                     IAHGMYTKARCLAALDARLPDEYTVDVQFGRPILLPATVQVSITRAGEGWKIAVHNPQ
                     GRPHLTGNISA"
     gene            complement(1398..>1692)
                     /locus_tag="VHH53_11140"
     CDS             complement(1398..>1692)
                     /locus_tag="VHH53_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2290721.1"
                     /translation="TNYATSKAGVIGLTRAQAAVLGERGITVNAVAPGFIETEMTARM
                     PLVLRQAGRRLNSLAQGGLPVDVAETVAWLAAPASAGVTGQVIRVCGQSLMGA"
BASE COUNT          270 a          554 c          563 g          305 t
ORIGIN      
        1 cgctggtagt cacgcattgc ggcgtcgagg aaccgccgtg gtttccggtg atccaggttt
       61 cgtacaactc accgtcacca tcgccgccgg tgagtcgcca gtggatcgcg gagtcgcgct
      121 gcggcgcgtt ttccgggcgg aactgatcgg ccatccggct gaagatccga tccagcagcg
      181 cacggcgcaa cgtcgaatca tccagtaccg cgtcgagttg ctcgttcgag gcgtatttga
      241 tcaacttggc gaaggcacgg gggtccatca cgcctctgcc attgccggtg ccaggcgtga
      301 gtagctgtgc gaattccgct gtggagaggt tcctcgggtc gatgttattg acgtccacca
      361 tggttactcc cttatctact agcagcctgt tggcgttctt cgagttcctc gctgagcatc
      421 gtgacggctg gatggtgctc taccgctaag ccgggggcac ggcaggttcg ccggggagat
      481 ctccgcggcc cggggccggt ctcgggcact actaccactg acatgcggac tacctcacgc
      541 actgatgttg ccagtgaggt gcggacgccc ctgaggattg tgcaccgcga ttttccaccc
      601 ctcgccggcc ctcgtaatgc tcacttgcac agtggccggc agcagtatcg gacggccaaa
      661 ttggacgtcc actgtgtatt cgtcgggaag ccgggcatcc agcgcagcga ggcatcgagc
      721 tttggtgtac atcccatgcg cgatcgcttg cgggaatccg aacccgcggg cggtcagccg
      781 gtgcaggtgg atcgggttgt agtccccgga cactgctgca tagcggcggc cgacgtctcc
      841 ggccagctgc cacacggccg tgggctccgg cggcgattga gccacggcgt ggccggacct
      901 gcgagctttg tcccggtggc cgctgcggta caggtaggcg ctccgttcgg accacaccag
      961 tgcaccgtcc gcagtgacct gcgtgatcac ggtgaactgg ctgcctcgat catgcggtct
     1021 caggtcgatg gcgcgtacct gcaatgtcaa tcgctcgtcg gcgcggatca cgcggcgtac
     1081 ggtgatccgg ttgccgatgt gcaccagccc cagcaagggg aaggggaagt tccggccggt
     1141 catcagctgc agcaccagcc cgaagctcag cacgtgcggg taggtgaccg gtagccggtc
     1201 agagagcgga aagccacata cccgcgcata ggccgccaag cgatgacggt cgacgatctg
     1261 ggcaggcagc gccagcgcgg tatccggtag ttgcgggggt cgccgggggc caggcaaggc
     1321 acctagcgcc gccttggtga acagcggcac ggtcgcgggc ggcgtggcca actcgatcat
     1381 ctcggtcgcc gcctccatca ggctcccatc aggctctgcc cacatacccg gatgacctgc
     1441 ccggtcactc cggccgaggc gggggcggcc aaccaggcca ccgtctctgc gacgtcaacc
     1501 ggcagcccac cctgagccag cgagttcagc cgccggcctg cctgccgcag caccagcggc
     1561 atccgggcgg tcatctcggt ctcgatgaac cccggggcga ccgcgttgac ggtgatgccg
     1621 cgttcgccca gcaccgctgc ctgtgcccgg gtcagcccga tgacccctgc cttggaggtc
     1681 gcgtagttgg tc
//
LOCUS       DATADN010000859         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_563231, whole genome shotgun sequence.
ACCESSION   DATADN010000859 DATADN010000000
VERSION     DATADN010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563231"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>1681
                     /locus_tag="VHH53_11145"
     CDS             60..>1681
                     /locus_tag="VHH53_11145"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2290722.1"
                     /translation="MSGGDSLPCDGGSSTLFTGDGEMATQIQTLDWSATPVGPVHAWS
                     QGLCTAVGIMLESRFPMLIWWGPELTMIYNDAYVPILGDKHPASLGRLGADVWSDVWP
                     VVGDMLAGVMRRGEATYDEDLLLMMLRHGYLEEAYFTFSYSPIRDETGGVGGVLTTVT
                     ETTGRVLGERRLRTLRELGELAAAEAATVSDACAAALRVLARNRTDVPYALAYVLDDE
                     ATSAGLIGSFGLRADNPMVPASLPHPSYGAEIWEVATTGQAALISGVDRDTFLGRDGV
                     IGKAPPHTAVALPLVAGGRPTGALVAGVSPYRALDGDYRSFFDLVAGQLSTVVADARA
                     YQAQRRRVEALAQLDRVKTDFFANISHEFRTPLTLLMGPLAELRAAPAVVADARTREN
                     VEMVHRSGLRLGKLVNSLLDFSRIQAGRLEASYEPVDLARFTLELASVFRAAVERAGL
                     VFELDCPPLGEVVYVDRDMWEKIVLNLLSNALKFTFDGRITVSLRAEMSSATSSALSR
                     VAVLRVADSGTGVGAEDLPRLFERFYRAEHAAA"
BASE COUNT          234 a          507 c          631 g          309 t
ORIGIN      
        1 gtgcactgct ggctagcgat ctttggttac ctctgactcc gatcgggagg cggtgcgcgg
       61 tgagcggcgg cgacagcctc ccgtgcgatg gcgggtcgtc gaccttgttc accggcgacg
      121 gcgagatggc aacccagata cagacgttgg attggtctgc cacgccggtg ggcccggtac
      181 acgcgtggtc acagggtctg tgcaccgccg tgggcatcat gctggagtcc cggttcccca
      241 tgctgatctg gtgggggccg gaactgacga tgatctacaa cgatgcctat gtgccgatcc
      301 tgggagacaa gcacccggcg tcactgggcc ggctcggcgc cgacgtgtgg tcggatgtgt
      361 ggcccgtagt cggtgacatg ttggccggag tcatgcggcg tggcgaagcg acctatgacg
      421 aggacctgct gctgatgatg ctgcggcacg gctatctgga ggaggcctac ttcacctttt
      481 cctacagccc gatccgcgac gagaccgggg gcgtgggagg ggtgctcacc acggtcaccg
      541 agacgaccgg gcgggtgctg ggcgagcgcc ggttgcggac gttgcgggag ttgggtgagc
      601 ttgccgccgc ggaggcggcc acggtctctg atgcctgcgc agcggcgctg cgcgtcttgg
      661 cccgtaatcg cactgacgtg ccgtacgcgc tggcctatgt gctcgatgac gaggcgacca
      721 gcgccggctt gatcggttcg ttcggcttgc gggccgacaa cccaatggtt cctgcgtcgc
      781 tgccccatcc aagctatggc gccgagatct gggaagtggc gaccactggc caggcggcgc
      841 tgatcagcgg tgtggatcgg gacacgttcc tcggccgaga cggcgtgatc ggcaaggcgc
      901 cgccccacac cgccgtggcg ctgccgctgg tggccggcgg ccggccgacg ggggcgttgg
      961 tcgctggggt gagcccctat cgggcgttgg atggcgatta ccggtcgttc ttcgacctgg
     1021 tcgcgggtca gctctcgaca gtggtcgccg acgcgcgggc ctaccaggcg cagcggcgac
     1081 gggtggaggc gctggcacaa ctggaccggg tcaagaccga cttcttcgcc aacatcagcc
     1141 acgagttccg cacgcccctg acattgctca tgggtccgct cgcggagctg cgggccgcgc
     1201 cggcggtggt cgcggacgcc cggacgcgcg agaacgtgga gatggtgcat cgcagtgggt
     1261 tgcggctggg caagctggtg aacagcttgc tggacttctc ccgtatccag gcaggccggc
     1321 tggaggcttc ctacgagccg gtggacctgg ccaggttcac cctggagctg gccagcgtgt
     1381 tccgggcagc ggtcgagcgg gccgggttgg tgttcgagtt ggactgccca ccgcttggcg
     1441 aggtggtgta cgtcgaccgg gacatgtggg aaaagatcgt tctcaacctg ctgtccaacg
     1501 cgctgaagtt caccttcgac gggcgcatca cggtgtcgtt gcgggctgag atgtcctcgg
     1561 cgacctcctc ggcgttgtcg cgggtggcgg tgttgcgggt ggctgactcc ggaaccgggg
     1621 ttggggccga ggacctgccg cggctgttcg agcgtttcta ccgcgcggag catgccgcag
     1681 c
//
LOCUS       DATADN010000860         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1301200, whole genome shotgun sequence.
ACCESSION   DATADN010000860 DATADN010000000
VERSION     DATADN010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301200"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /gene="tatC"
                     /locus_tag="VHH53_11150"
     CDS             complement(<1..792)
                     /gene="tatC"
                     /locus_tag="VHH53_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX2290723.1"
                     /translation="MRFPAGRFLAAPGRRRKANPDGSMTLMEHLYELRYRLFVALAAV
                     ALGGILGFTWWGTSAFGLPTLGDLITRPYCGLPETLRLTPNQGRCQLLQTGPFEVFTL
                     RMQVGLATGAVLTAPVWLYQLWAFITPGLYAKERKFAVVFVGASTVLFAAGAVLAYYV
                     VPTGLSFLANLGAGQFITALTGKAYVGFLLTMLVVFGLAFELPLLVVMLNRVGVLPYE
                     KLKRWQRGILFGLFVLAAVATPGSDPISMLALAGALVVLFEMSVLI"
     gene            complement(815..1114)
                     /gene="tatA"
                     /locus_tag="VHH53_11155"
     CDS             complement(815..1114)
                     /gene="tatA"
                     /locus_tag="VHH53_11155"
                     /inference="COORDINATES: protein motif:HMM:NF001854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX2290724.1"
                     /translation="MPNLGGWELIVLLGVLVLLFGATKLPAAARAIGQSMRILKAETK
                     GMRSEGEATPPASPEQAPQQVLPPSGPTTAPVQEPTAVQEPTAVQKSQRHNDTTR"
     gene            complement(1152..1388)
                     /locus_tag="VHH53_11160"
     CDS             complement(1152..1388)
                     /locus_tag="VHH53_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290725.1"
                     /translation="MPYLISALVAAAGAVVLLMLLMRLLGPVRRLARTARNSRAGFAD
                     RSRELTARIAALRVELARRRRRRNAEPPSAAPAA"
     gene            complement(1399..>2350)
                     /locus_tag="VHH53_11165"
     CDS             complement(1399..>2350)
                     /locus_tag="VHH53_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX2290726.1"
                     /translation="AERLPRLLALVPYLLARPGAPIEDVAADFDVTVRQLRRDLELLW
                     MCGLPGYGPGDLIDLSFEGDTVTVTFDAGMRRPLRLSGAEATALVVALRALAETPGVV
                     DAGSVRRALAKIEAAAGHPDGVVVGLATSEEAALAAVRDALDRRRALRIRYYTASRDA
                     VGGRTVDPMRLLLVEGRSYLEAWCRSAEGVRLFRLDRIEHVATLDETAAPPPHARPTD
                     VSAGLFRAQPDQQVAVLALAPDARWVGEYYPVEELTEGEGGAATVRMRYADTGWMVRL
                     VLGLGADVTVRQPPELAAAVTQRARAALHRSGQLASDEQG"
BASE COUNT          396 a          822 c          818 g          314 t
ORIGIN      
        1 gatcagcacc gacatctcga acagcaccac cagcgcaccg gccagcgcca gcatggaaat
       61 cgggtcactg ccgggggtgg cgacggcagc aagcacgaac agcccgaaca ggatgccgcg
      121 ctgccagcgc ttgagctttt catagggcag caccccgacc cggttgagca tcaccaccag
      181 cagcggcaat tcgaaggcca gaccgaagac caccagcatc gtcagcagga agccgacata
      241 ggccttgccg gtcagcgccg tgatgaactg cccggcgccc aggttcgcca ggaaggacag
      301 gccggtgggg accacgtagt aggcgaggac cgcgccggcg gcgaacagca cagtcgacgc
      361 gccgacgaag acgacggcga acttgcgttc cttggcatag aggccgggtg tgatgaacgc
      421 ccacagctgg tacagccaca ccggcgcggt gagcaccgca ccggtcgcca agcccacctg
      481 catccggagg gtgaacacct cgaacgggcc ggtctgcagc aactggcacc ggccctggtt
      541 cggggtcagc cgcagcgtct ccggcaggcc gcagtacgga cgggtgatca ggtcgcccaa
      601 agtgggcagc ccgaatgctg aggtccccca ccaggtgaac cccaggatgc caccgagtgc
      661 gaccgctgcc agcgcgacga agagccggta gcgcagctcg tagaggtgct ccatgagcgt
      721 catggagccg tccgggttgg ccttgcgccg gcggcccggc gcagcgagga aacgtccagc
      781 cgggaaacgc atcccgctca cccgaccatt gtcgtcagcg ggtggtgtcg ttgtgacgct
      841 gagacttctg gacggccgtt ggctcctgaa cggccgttgg ttcctgaacc ggcgccgtgg
      901 tgggacctga cggcggcagc acctgctgag gggcctgctc gggggatgcc ggcggggtcg
      961 cctcgccctc cgaacgcatg cccttggtct cggccttcaa gatgcgcatg gactgcccga
     1021 tggcccgggc agccgccggc agcttggtcg cgccgaacag caggaccagc acgcccagga
     1081 gcactatcag ctcccagcca cccagattag gcatcgtggc ttcctttctc gtttctcgtg
     1141 cgggaccgat gctacgcggc cggggctgcg ctggggggct ccgcgttgcg gcggcgacgc
     1201 cgccgggcga gctcaacccg tagtgccgcg atccgggcgg tcagctcgcg gctgcgatcg
     1261 gcaaagccgg cgcggctgtt gcgggcagtc ctagccagcc ggcggaccgg gccgagcaat
     1321 cgcatcagca gcatgagcag caccacggcg ccggcggcgg ccaccagtgc actgatcagg
     1381 tagggcacgg agatgacgtt agccctgctc gtccgatgcc agctgaccgg atcggtgcag
     1441 cgccgcgcgg gctcgctggg tgactgccgc agccagttcg gggggctgcc ggaccgtcac
     1501 gtcggcaccc agccccagca ccaggcgaac catccagccg gtgtcggcgt agcgcatccg
     1561 caccgttgcg gctccccctt cgccctcggt tagctcctct accgggtagt actcgccaac
     1621 ccagcgagca tccggggcca gtgcgagcac ggcgacctgc tggtcgggtt gtgcgcggaa
     1681 cagcccggca gaaacgtcgg tgggacgggc atgtggtggt ggcgcggccg tctcgtccag
     1741 tgtcgcgacg tgctcgatgc ggtccagccg gaacaggcgc actccctcgg cgctgcggca
     1801 ccaggcctcc aggtagctgc ggccttcgac cagcagcagc cgcatcggat ccacggtgcg
     1861 ccctcccacc gcgtcgcggg aagcggtgta gtagcggatg cgcagcgcgc ggcggcggtc
     1921 cagggcgtca cgcaccgcgg ccagggcagc ttcttcgctg gtagccaacc cgactaccac
     1981 tccatcgggg tgcccagctg ccgcctcgat cttggccagg gcccggcgca ccgatccggc
     2041 gtcgaccaca cccggtgtct cggccagcgc tcgcagcgcc accaccaacg cggtggcctc
     2101 cgccccggac agtcgaagcg ggcggcgcat cccggcgtcg aaggtgacgg tcacggtgtc
     2161 accctcgaag gacaggtcga tgaggtcgcc gggcccgtag ccgggcaacc cgcacatcca
     2221 cagcagctcc aggtcgcggc gcagctggcg gacagtgacg tcgaagtcgg cggccacgtc
     2281 ctcgatcggc gcacctggcc gcgccaacag gtagggaacc agggccagga gccggggcaa
     2341 tcgctcggcg
//
LOCUS       DATADN010000861         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1019926, whole genome shotgun sequence.
ACCESSION   DATADN010000861 DATADN010000000
VERSION     DATADN010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019926"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..256)
                     /locus_tag="VHH53_11170"
     CDS             complement(65..256)
                     /locus_tag="VHH53_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290727.1"
                     /translation="MTKRTDRIDPTWPSWSEEDHPVSELAAPIQGALSPFGEVTFPLP
                     ADQLPYQHPHTEINRSDTP"
     gene            364..>2123
                     /locus_tag="VHH53_11175"
     CDS             364..>2123
                     /locus_tag="VHH53_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEX2290728.1"
                     /translation="MPSTALADWLRSRDDAALAALLRARPDLAVPPPADSTVLATRAG
                     IQASVARACEDLDRFTLIVLSALVVAGADTESVSRQDVAGLLGRDVTTGRIQQGLDAL
                     RCRALAWGEDTALAVVPAARQVIGPHPGGLGRRAADLAGVDIGAAIAALAEDELGVLR
                     TLAAGPPVGTTRDAAEVLALSRARTPVQRLLARGLLRRIDSGTVELPAQVGLALRGNQ
                     PLGPIDLEEPALPRQQRDPSMVDSTAAGAVLDLLRHTEKLLSEWSREPAAALRSGGLG
                     IRDMRRTARQLDVDESTVALLAEVTVAAGLVAESDEGAEGRLWLPTVAIDSWLAARPE
                     RRWAMLANAWLELPRLPGLAGIRDDKDRPLAALSDELRQPRAAEQRRRVLDVLADLPA
                     GSGIQRSDELAAVLAWRAPRRGGRRRDEMVSWTVREATVLGVLALGALSSPGRALLQD
                     GAGAAAAVLEATLPEPVDHVLVQADRTVVAPGPLEPELARAIGLVADVESAGGATVYR
                     VTEDTVRRALDVGRSATDLHELFRTRSRTPVPQSLSYLIDDVARRHGRLRGGTAAAFL
                     RCDDVVLLAELLAHPVAARCG"
BASE COUNT          271 a          716 c          787 g          349 t
ORIGIN      
        1 cgggacccta acggatccga accgacgcct ttaacagacc ctctgcgagg ccccctgtgg
       61 cgctctaagg ggtgtcgctg cggttgatct ccgtgtgcgg gtgctggtag gggagctggt
      121 cagccggtag cgggaaggtc acctcgccaa agggggacag cgcgccttgg atcggggcgg
      181 cgagttcgct gacagggtgg tcctcttccg accagctcgg ccaggtcggg tcgattcggt
      241 cggtgcgctt ggtcacagct tctcctcgct cggtggtatt tccattgtgc cccggggtca
      301 cacagttcat tgtgatcagt ggcgccatgc gacgtgtcct gggctgtcga taccgtggat
      361 gggatgccca gcactgcact ggccgactgg ctacgctccc gggacgacgc cgcgctggcc
      421 gcgttactac gcgcccgccc ggaccttgcc gtgccgcctc cggctgattc gacggtgctg
      481 gccacccgag cggggatcca ggcatccgtc gcccgggcgt gcgaagactt ggatcggttc
      541 acgctgatag tactgtcggc gctggtagta gcgggtgccg acaccgaatc ggtgagccgc
      601 caggacgtcg ctggactgct cggtcgagac gtcaccaccg gccgaattca gcagggcctg
      661 gacgcattgc gctgccgcgc actggcctgg ggtgaggaca cggcgctcgc ggtggtccca
      721 gcggcgcgcc aggtcatcgg tccacatcct ggcgggttgg gccgtcgcgc cgcggatctg
      781 gccggcgttg atatcggcgc cgccatcgcc gcgctcgccg aggacgagct gggtgtgctg
      841 cggaccttgg ccgccgggcc ccccgtgggc accacccggg atgccgccga ggtgcttgcg
      901 ctgagccggg cccgcacgcc ggtgcaacga ctactggccc gcgggttgct acgccggatt
      961 gactccggca ccgtcgagtt gcccgcacag gtgggcttgg cgttgcgcgg gaaccaaccg
     1021 ctcgggccca tcgatctgga ggaaccggcg ctgcccaggc agcagcgcga cccgtccatg
     1081 gtggactcga cggccgccgg cgcggtgctc gatctgctcc ggcacaccga gaagctgctc
     1141 tccgaatggt cgcgagaacc ggctgctgcg ttgcgctccg gcggcctggg catccgggac
     1201 atgcggcgca ccgcgcggca gctggacgtc gatgaatcca cggtagcgct gctcgccgag
     1261 gtgaccgtcg cggctgggct ggtcgctgag tccgacgagg gcgccgaggg caggctgtgg
     1321 ttacccacgg tcgccatcga ctcctggctt gctgcgcgac ccgaaaggcg ctgggcgatg
     1381 ctggccaacg catggcttga gttaccccgg ctgccgggat tggccgggat tcgcgacgac
     1441 aaggaccggc cgctggcagc gctgtctgat gagttgcgcc agccacgcgc cgcggagcaa
     1501 cgccgccggg tcctcgacgt gctcgccgat ctgcccgcgg gtagcgggat ccagcggtcc
     1561 gacgagctcg cagcagtgct cgcctggcgg gccccgcgtc ggggtggccg gcgacgcgat
     1621 gagatggtca gctggacggt ccgggaggcc accgtgctcg gcgtgctcgc gctgggcgcg
     1681 ctgtcatcgc ccggccgggc gctgctgcag gacggcgctg gcgccgcggc ggccgtcctc
     1741 gaagccacct tgcccgagcc ggtggaccac gtgctggtgc aggccgaccg caccgtagtg
     1801 gcgccgggac cgttggagcc cgagctggcc agggctatcg ggctggtcgc tgacgtggag
     1861 tcggccggcg gcgccaccgt ctaccgggtc accgaggaca ccgtgcgccg cgccttggac
     1921 gtgggccgta gcgccaccga cctgcacgaa ctgtttcgca cccggtcccg gactccggtg
     1981 ccgcagtcgc tgagctacct catcgatgat gtggcccgac ggcacgggcg gttgcggggt
     2041 ggcaccgcgg ccgcattcct gcgttgcgac gacgttgtgt tgctggctga attgctggcg
     2101 catccggtcg ccgcgcgctg cgg
//
LOCUS       DATADN010000862         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1469942, whole genome shotgun sequence.
ACCESSION   DATADN010000862 DATADN010000000
VERSION     DATADN010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469942"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1812
                     /locus_tag="VHH53_11180"
     CDS             <1..1812
                     /locus_tag="VHH53_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2290729.1"
                     /translation="VVLTELAGDALRFVAVPDDPARDWALLATPLANVLEPIGPEAAA
                     MGYGVFAWVHIGFVLVFGTYLPYSKHLHILTSEPNVYFRNLEPRGALRKMDLEAEPVD
                     GVEPTFGAKTLTDLSWRHLLDPMSCTECGRCMEFCPASMTGKTLSPKHFMEGLRDQIA
                     MAETALAAAAGAQRAAKGGASGGVEASDASLALARERAEEALTLPIVDNAIPEEAIWQ
                     CTTCGWCVEGCPVLIEHVDSIVEIRRNLVLEEGRNPKELNAAFRNMETAGNPWGQPRG
                     GRLDWAKGLDVPVLGQSPATPSAPTPQADDNRAGRRWRTIYGRGREAPILYWVGCAGA
                     FDDRNRRVVRAMAQLLAQAEVPFAVLGPGETCSGDPARRAGNEYLFQMLAEENVTTLN
                     QAAVDHGIHTIVASCPHCFNTIRNEYAQFGLGRFDVIHHTQLLDTLVAQGRLNLTEHR
                     DAVVAYHDACYLGRYNDVYEPGRRIVASVPGHEVAEMSLHHRKGMCCGAGGARMWMEE
                     REGERINHRRTHQALETDATAIATACPYCLIMLRDGVTDLGRTDVAVSDVAELLADAT
                     GAWNRATSEENADRPVLSPLAGGNGDETAAEGPTRAG"
BASE COUNT          302 a          631 c          655 g          252 t
ORIGIN      
        1 gtcgtcctca ccgagctcgc gggtgacgcc ctgcgtttcg tcgccgtccc agacgacccg
       61 gcacgcgact gggcgctgct ggccacaccg ctggcgaacg tgctggagcc catcgggccc
      121 gaagccgccg caatggggta cggcgtcttc gcctgggtgc atatcggctt tgtgctcgtg
      181 ttcggcacgt acctgccgta cagcaagcac ctccacatcc tgaccagcga gccgaacgtg
      241 tacttccgca acctggagcc gcgcggcgcc ctgcgcaaga tggacctgga ggcggagccg
      301 gtggatggcg tggagcccac gttcggcgcc aagacgctga ccgacctgag ctggcgccac
      361 ctgctcgacc cgatgagctg caccgagtgc ggtcgctgca tggagttctg cccggcatcg
      421 atgacgggca agacgctgag cccgaagcac ttcatggagg gattgcgcga ccagatcgcc
      481 atggccgaga ccgcgctggc cgcggccgcc ggagcgcagc gcgcggcgaa gggcggggcc
      541 agcggcggag tggaggcatc ggacgcctcg ctggctctgg cccgggagcg ggcggaggaa
      601 gccctgacgc tgccgatcgt ggacaacgcc atccccgagg aggcgatctg gcagtgcacg
      661 acctgcggct ggtgcgtgga gggctgcccg gtgctcatcg agcatgtcga cagcatcgtg
      721 gagatccgac gcaacctcgt cctggaggag ggccgaaacc cgaaggagct caacgccgcc
      781 ttccgcaaca tggagaccgc cggcaatccc tggggccagc cccgcggcgg gcggctggac
      841 tgggccaagg ggctcgacgt gccggtactg gggcagtcgc cagccactcc atcggccccg
      901 acgccccagg ccgatgacaa ccgcgcgggc cggcgctggc gcaccatcta cggccggggt
      961 cgggaggcgc cgatcctgta ctgggtggga tgtgccgggg ccttcgacga ccgcaaccgg
     1021 cgcgtggtgc gggcaatggc ccagctgctg gcccaggcgg aggtgccgtt cgcggtgctg
     1081 ggcccgggcg aaacctgcag cggcgacccc gcgcggcgtg ccggcaacga gtacctgttc
     1141 cagatgctcg ccgaggagaa cgtgacgacc ctgaaccagg ccgcggtgga ccatggcatc
     1201 cacaccatcg tggccagctg cccgcactgc ttcaacacaa tccgtaacga gtacgcgcag
     1261 ttcgggctgg ggcgcttcga cgtgatccac cacacccagc tgctcgacac cttggtggcc
     1321 cagggcaggc tcaacctgac ggagcaccgg gatgcagtgg tcgcctacca cgacgcctgc
     1381 tacctgggcc gctacaacga cgtgtacgag ccggggcgcc ggatcgtcgc ctcggtcccg
     1441 ggccacgagg tggccgagat gagcctgcac catcgcaagg gcatgtgctg cggcgcgggc
     1501 ggagcgcgga tgtggatgga ggagcgcgag ggggaacgca tcaaccatcg tcgcacgcac
     1561 caagcgctcg agaccgatgc gaccgccatc gcgaccgcgt gcccgtactg cctgatcatg
     1621 ctgcgcgacg gcgtgacgga cctgggccgg accgatgtcg ccgtctcgga cgtcgcggag
     1681 ctgctggcgg atgccaccgg cgcctggaac cgcgccacct ccgaggaaaa cgctgaccgg
     1741 cccgtgcttt caccacttgc tggtggtaac ggagacgaaa cggctgccga gggccccaca
     1801 cgagccggct aaggccgtgc gcgtcgctcc tgtcagtcct
//
LOCUS       DATADN010000863         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1470117, whole genome shotgun sequence.
ACCESSION   DATADN010000863 DATADN010000000
VERSION     DATADN010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470117"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VHH53_11185"
     CDS             complement(<1..1124)
                     /locus_tag="VHH53_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290730.1"
                     /translation="MSAARYPFSAIVGHDDLRLALLLTAVHPGIGGVLVRGEKGTAKS
                     TVVRALAALLPPVTAVPGCRFGCDPVVPDLGCPDGPHAASAGHSARPARLVELPVGAT
                     EDRLVGSLDLQRALAEGISVYQPGLLAAAHRGVLYVDEVNLLPDHLVDVLLDAAAMGR
                     SHVERDGVSVSHAASFLLVGTMNPEEGELRPQLLDRFGVTVEVAASRDVGTRAEVIRR
                     RLCYEADPAGFAAGWRGEDVALAQRIAVARSVLPRVTLPDGELRRIASVCAAFEVDGM
                     RADLVLARTALANAAWRGADVVSEDDVRIAARLALPHRCRRDPFDQPGLDNEQLEDAL
                     QDADHTPEDPPNGPGGGNASPDMEAYRGDSAADNPGMPPCR"
     gene            complement(1126..>1541)
                     /locus_tag="VHH53_11190"
     CDS             complement(1126..>1541)
                     /locus_tag="VHH53_11190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2290731.1"
                     /translation="MIYALLRLRVEVFIVEQRCPYQDLDGHDLHQTTRHYWLAPYGAI
                     EDAQATLRLLEPTEGQFKIGRVCAARSVRAQGAVRRLLEAALAEVGDAPCLLDAQTDS
                     VGFYAEFGFVVEGAEFTEDGVPHVLMRRAGSARVR"
BASE COUNT          266 a          556 c          509 g          210 t
ORIGIN      
        1 cgacatggag gcataccggg gttatccgct gccgaatcac cccgatatgc ctccatgtcg
       61 ggactggcat tacctccgcc ggggccattg ggggggtctt ctggggtgtg gtccgcatcc
      121 tgcagggcgt cttcgagctg ctcgttgtcc agaccgggct ggtcgaaggg gtcgcggcgg
      181 cagcggtgcg gcaacgccaa ccgggcggcg atccggacgt cgtcctcgct gaccacgtcg
      241 gctccccgcc aggcagcgtt cgccagagcg gtgcgagcaa ggaccagatc agcgcgcatc
      301 ccgtccactt cgaacgcggc acacaccgac gcgatccggc gtaactcgcc atccggcagc
      361 gtcacgcgcg gcaacaccga ccgggccacc gcgatccgct gcgccagcgc gacgtcctcg
      421 ccacgccaac ctgccgcgaa gccggccgga tccgcctcgt agcacaaccg gcggcggatc
      481 acctcggctc gcgtcccgac atcccgggaa gcggctacct ccacggtcac accgaagcga
      541 tccagcagct gcgggcgcag ctcgccctct tctgggttca tcgtgccgac cagcaggaac
      601 gacgcggcat gcgagaccga cacgccgtcg cgctccacgt gcgagcgacc catcgcggca
      661 gcgtcgagca gcacgtcgac aagatggtca ggcagcagat tgacctcgtc gacgtagagc
      721 acgccgcgat gcgctgcggc gagtagcccg ggctggtaaa ccgagatgcc ctcggccagg
      781 gcccgctgca ggtccagcga acccaccagc cggtcctcgg tagcaccgac gggcagctcg
      841 accagccgag cggggcgggc gctgtgccct gctgaggcag catgtggccc gtcggggcag
      901 cccagatctg ggaccaccgg gtcacaaccg aagcggcatc ccggtaccgc ggtcaccggg
      961 ggcagtaacg cggccagcgc ccgcaccacc gttgatttcg cggtgccctt ctcaccgcgg
     1021 accagtacac cgccgattcc tggatgcacc gcagtcagca gtaacgccag ccggagatcg
     1081 tcgtgcccga caatcgcgga gaacgggtac cgggcagcgc tcacgtcagc ggaccctcgc
     1141 tgatcccgcg cggcgcatca gcacgtgcgg gacaccgtcc tcggtgaact ccgccccttc
     1201 caccacgaag ccgaactcgg cgtagaagcc gaccgagtcg gtctgcgcgt cgagcaagca
     1261 gggggcgtca cccacttcgg ccagcgcggc ctcgagcagc cgccggactg cgccttgggc
     1321 ccgaacggac cgggcggcgc agacccggcc gatcttgaac tggccctcgg tgggctccag
     1381 cagccgcagg gtggcctggg cgtcctcgat agcgccgtag ggtgcgagcc agtagtgccg
     1441 cgttgtctgg tgcaggtcat gaccgtccag atcctggtag ggacagcgtt gctcaacgat
     1501 gaacacctct acccgcagcc gcaacaaagc gtagatcatg g
//
LOCUS       DATADN010000864         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1301491, whole genome shotgun sequence.
ACCESSION   DATADN010000864 DATADN010000000
VERSION     DATADN010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301491"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="VHH53_11195"
     CDS             complement(<1..1665)
                     /locus_tag="VHH53_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX2290732.1"
                     /translation="MAQRLQLLLAGIVEGPDRPLGQLPWMSEAERHQVLHAWNDTGLD
                     VPAVVFPQVLQAQVAQTPQLTALVFGDSVLSFAELNARANRLARLLIDYGAGPEQVVA
                     LVLPRSVEMIVALVAVFKAGGVYLPVDPQLPADRIGFMIADAAPVLVVTTTAVAAVTE
                     VTAVGAPVVVVDDPATATVLEGYSDADVTDADRLGPLSVDSSAYVIYTSGSTGRPKGV
                     LVEHRNVMNLFYHHQAQVRPGAGGRLRVALSTALSFDASLDAVLSMTAGHELHLLDEV
                     VRLDPEAVVEYVTAHRIDHVNFTPSFVTQLLAAGLLTNQRHRPGVLVLGGEAISEALW
                     TQLGEAADTTGYNFYGPTETTIVALSCRIEQGMRPAIGRPLRNLRAYVLDHALRPVPV
                     GVAGELYLAGAQVARGYLHRPGLTAARFLACPFEAPGTRMYATGDLVRWRADGQLEYL
                     GRTDDQVKIRGFRIEPGEIETVLVGHSEVGEAAVIVREDQPDAKRLVAYVVPVADGVV
                     DSTGLRAHVASRLPDYMVPAAFVTLDELPLSPNGKLDRKALPAPEFA"
BASE COUNT          346 a          627 c          487 g          245 t
ORIGIN      
        1 agcgaactcc ggcgccggca acgctttccg gtccaacttc ccattcggac tcaacggcaa
       61 ctcgtccagc gtcacgaacg ccgccggcac catgtaatcc ggcaacctgg atgccacatg
      121 cgcccgcaac ccagtagaat ccaccacccc atcagcaaca ggcaccacat aggccaccag
      181 ccgcttcgca tccggctggt cctcgcggac aatcactgct gcctcaccga cttcggaatg
      241 ccccaccaga acggtttcga tttcgccggg ttcgatgcgg aacccgcgga ttttgacttg
      301 gtcgtcggtg cgcccgaggt attccagttg cccatcagcg cgccagcgga ccaggtcccc
      361 ggtggcgtac atgcgggtcc cgggcgcctc gaagggacag gccaggaacc gggccgcggt
      421 cagcccgggg cggtggaggt atccgcgggc tacttgggcg ccggcgaggt acagctcccc
      481 ggccaccccg acgggcaccg gacgcagcgc gtgatcaagc acataggccc gcaggttgcg
      541 cagcgggcgc ccgatcgccg ggcgcatccc ctgctcgatg cggcaggaca acgccacgat
      601 ggtggtttcg gtggggccat agaagttata gccggtggtg tcagcagcct cccctagctg
      661 ggtccacagc gcctcactga tcgcttcgcc acccaagacc agcaccccgg gccggtgccg
      721 ttggttggtc aacaacccgg ccgccagcag ctgggtaaca aaagacggcg tgaagttcac
      781 gtgatcgatg cggtgggcgg tcacatattc caccaccgcc tcggggtcta ggcgcaccac
      841 ctcatcaagc aggtgcagct catgacccgc ggtcatcgac aacaccgcgt caagcgaggc
      901 atcaaacgac aacgccgtgc tcaacgcgac ccgcaaccgg cccccggcgc cgggacggac
      961 ctgggcctga tggtgataaa acaggttcat cacattgcgg tgttcgacca gcacaccctt
     1021 ggggcgcccg gtggaccccg aggtgtagat cacatacgcc gagctgtcga cagacagcgg
     1081 tccgagccga tcagcatcgg tgacatccgc atcggaatag ccctccagca ccgtcgcggt
     1141 ggccgggtca tccaccacca ccaccggcgc accgaccgcg gtcacctcgg tcaccgccgc
     1201 gaccgcggtg gtggtcacca ccaacaccgg cgcagcatca gcaatcatga agccgatccg
     1261 gtcagctggc agctgcgggt ccaccggcag atacacccca ccggccttga acaccgccac
     1321 caacgccacg atcatctcca ccgaccgcgg caacaccagc gccaccacct gctcaggccc
     1381 ggccccatag tcgatcagca accgcgccaa ccgattcgcc cgagcgttta gctcggcgaa
     1441 actcaacacc gaatccccga acaccaacgc ggtcagctgc ggggtttggg ccacctgggc
     1501 ctgcaacacc tgcgggaaca ccaccgccgg cacatccaaa ccggtgtcat tccacgcatg
     1561 cagcacctga tggcgttcgg cctcagacat ccacggcaac tgccccaacg gccgatccgg
     1621 accctcaaca atcccggcca acaacaattg caaccgctgg gccatccgct cgatcgtagc
     1681 cgcatcgaat aggtcggtgt tgtag
//
LOCUS       DATADN010000865         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_417447, whole genome shotgun sequence.
ACCESSION   DATADN010000865 DATADN010000000
VERSION     DATADN010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417447"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VHH53_11200"
     CDS             <1..849
                     /locus_tag="VHH53_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2290733.1"
                     /translation="IFMAVRRESRFPISEILAQTPPIPAGAQWGIFLRNHDELTLEMV
                     TDEERDYMYAEYAKDPRMKANIGIRRRLAPLLDNDRNQQELFTALLMSLPGSPVLYYG
                     DEIGMGDNIWLGDRDGVRTPMQWTPDRNAGFSRCDPGRLYLPVIADPVYGYQSVNVEA
                     QLNNTSSLLHWTRRMLQVRREHTAFGLGDFTELGSSNPSVLAYLRRWVDGGGEEDVVV
                     CVNNLSRFPQPVELDLSDHAGCTPVELTGGVPFPPIGELPYLLTLPGHGFYWFQITTG
                     EGEVSL"
     gene            846..>1523
                     /locus_tag="VHH53_11205"
     CDS             846..>1523
                     /locus_tag="VHH53_11205"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEX2290734.1"
                     /translation="MTGLRPNELVNDLLEAIRHWLPAQRWFAAKRRGITSIEPRHAAT
                     IIDGHAGLVQALVDVDGALYHLLLGVRDELAEHVAHAVIGHVDGWHVYDAPHDPELMG
                     HLLDLLASDTDSGGLRFRHEPWAELRRGLRARPIGVEQSNTSVIYGHHYILKLFRRPT
                     AGPNRDVDLHRALHDAGSTHIAEPLGTIEGHLADAPDVPMTFGFLQRFLPDAVEGWAT
                     ATASVRDL"
BASE COUNT          240 a          549 c          510 g          224 t
ORIGIN      
        1 atcttcatgg cggtccggcg cgagtcgcgg ttcccgatct cggagatcct cgcccagaca
       61 ccgccgatcc cggcgggggc gcagtggggc atcttcctgc gcaaccacga cgagctgacc
      121 ctcgagatgg tcaccgacga ggaacgcgac tacatgtacg ccgagtacgc caaagatccg
      181 cggatgaagg cgaacatcgg catccggcgc cggctggctc cgctgctcga caacgaccgc
      241 aaccagcagg agctgttcac cgcgttactg atgtcgctac ccggctcgcc cgtgctgtac
      301 tacggcgacg agatcggcat gggcgacaac atctggctcg gcgaccgcga cggggtgcgc
      361 accccgatgc agtggacgcc ggaccgcaac gccggcttct cccggtgcga tcccggccgg
      421 ctgtacctgc cggtgatcgc ggacccggtg tacggctacc agtccgtgaa cgtggaggca
      481 cagctcaaca acacctcctc gctgctgcac tggacccgcc ggatgctgca ggtccgtcgc
      541 gagcacaccg ccttcgggct cggcgacttc accgagctgg gctcgtccaa ccccagcgtg
      601 ctggcctacc tgcggcgctg ggtcgacggg ggcggcgagg aggacgtggt ggtctgcgtg
      661 aacaacctct cccgcttccc gcagccggtg gagctggacc tgtccgacca cgcggggtgc
      721 acgccggtcg agctcaccgg cggggtaccc ttcccgccga tcggcgaact cccctacctg
      781 ctgaccctgc ccggacacgg cttctactgg ttccagatca ccacagggga aggcgaggta
      841 agcctgtgac ggggttacga ccgaatgagc tggtcaacga cctgctggag gcgatccggc
      901 actggctgcc ggcccagcgg tggttcgccg ccaagcgccg gggcatcacc tcgatcgagc
      961 cccgccacgc cgccacgatc atcgacggcc atgccggcct ggtgcaggcg ctggtggacg
     1021 tggacggcgc gctctaccac ctgctgctcg gggtacgtga cgagctggcc gagcatgtcg
     1081 cgcatgccgt catcggccac gtggacggct ggcacgtcta cgacgcgccg catgatccgg
     1141 agctgatggg ccacctgctc gacctgctgg ccagcgacac cgacagcggt gggctgcggt
     1201 tccggcacga gccgtgggcc gagctgcggc gtgggttgcg cgcccggccg atcggggtcg
     1261 agcagagcaa cacctcggtg atctacggcc accactacat cctcaagctg ttccgccggc
     1321 caactgccgg ccccaaccgc gacgtggacc tgcaccgcgc gctgcacgac gcgggcagca
     1381 cgcacatcgc cgagccgctc ggcacgatcg agggccacct cgccgacgcg ccggacgtgc
     1441 ccatgacctt cggcttcctg cagcggttcc tgccggacgc cgtggagggc tgggccacgg
     1501 ccaccgccag cgtccgggat ctg
//
LOCUS       DATADN010000866         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_248747, whole genome shotgun sequence.
ACCESSION   DATADN010000866 DATADN010000000
VERSION     DATADN010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248747"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VHH53_11210"
     CDS             complement(<1..798)
                     /locus_tag="VHH53_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290735.1"
                     /translation="MLEQHGAIDTQQLAALGVTTDAITANVDAGRWRALLPRVYTTTT
                     GLPPRPTRLHAALLYAGPSGILSHRTAAEEWGMLPVEDGPVHVTVPYGCSAVSQPPEV
                     VVHRSRAFAYIAVASIPPRTSRADTALDLAVAEPSAREAMRRLVGVATTCGIPLIELE
                     RQLIERPPYRYRRALASALVRMAGGVASVLEDMYALDVEAAHAIPPAARQVPFEVDGY
                     TLWEDAVYDHLGVPLTVRLDGRAYHSHPGVAFRDRRRDNAAELAGRAR"
     gene            complement(987..>1925)
                     /locus_tag="VHH53_11215"
     CDS             complement(987..>1925)
                     /locus_tag="VHH53_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX2290736.1"
                     /translation="IARAYVTALRDLLRALDVSDVRMDQGSLRCDANVSLMLRGSSTF
                     GTRTETKNVNSLRSVERAVHHEMCRQAAVLASGGRIVQETRHFDEANGTTSPGRRKET
                     SEDYRYFPEPDLVPIAPAEEWVQRLRATLPELPWQRQARIQAQWGLSDLELRDLVNAG
                     ALDLIAATVEAGAHAAEARSWWVSYLSQQANSRGMELAELPITPEQVARVIALIGEGS
                     LTNNLARQVVDGVLAGEGEPDDVVAARGLAVVSDDAALTAAVEEALAAQPDIAEKIRG
                     GKVAAAGAIVGAVMKATGGQANAQRVREIVLALVSA"
BASE COUNT          307 a          676 c          641 g          301 t
ORIGIN      
        1 ccgcgctcta ccggccaact cggcggcgtt atcccggcgg cggtcacgga aggccacccc
       61 ggggtgcgag tgataagccc gaccatccag ccgcaccgtc agcggcacac cgaggtggtc
      121 gtacaccgcg tcctcccaca aggtgtaacc gtccacctcg aagggtactt ggcgcgctgc
      181 aggagggatg gcatgggcag cctcgacgtc cagcgcgtac atatcctcca gcaccgatgc
      241 cacgccaccc gccatccgta ccagagccga cgcgagcgcc cggcgatagc ggtacggggg
      301 ccgttcgatc agctgccgct caagctcaat cagtgggatg ccgcaggtcg tcgccacacc
      361 cactagccgg cgcatcgcct cacgtgcgct cggctcggca acggcaagat cgagggctgt
      421 gtcggcccgg ctggtcctgg gcggaatgct cgccaccgca atgtaggcga atgccctgga
      481 ccggtgcacc accacctccg gcggctggga taccgctgag cacccatagg gcaccgtgac
      541 gtgcaccggg ccgtcttcga ccggcagcat gccccattcc tccgcggcgg tgcggtggct
      601 caggatgccc gaagggccgg cgtagagcag tgcggcatgc aacctggtcg gtcttggagg
      661 caggccggtc gtcgtcgtgt agacccgagg cagcaacgct cgccatcggc cggcgtcaac
      721 gttggccgtg atggcgtctg tcgtgactcc gagcgcggca agctgttgcg tatcgatggc
      781 cccgtgctgc tccagcaaga gctgttccca ctcaaggagc tcgcgtacct ggtgcggggc
      841 aggccggact cgaacgatgc ggggtctggg catgtcggga cacgctgccg cagctacgtc
      901 ccgatgtccg tgctcctacc gccccgctgt ggacaaccac cgcagctgtg gataactcgt
      961 gagtggcatt cagtgctgga acactgttat gcactcacga gcgcgagcac gatctcgcgc
     1021 acgcgttggg cgttggcttg gccaccggtt gccttcatca ccgcgcccac gatggctccg
     1081 gcggcggcca ccttgccacc ccggatcttc tcggcgatgt cgggctgggc cgccagcgcc
     1141 tcctcgacgg cagcggtcag cgcggcgtca tcggacacca cggccagacc gcgggcggcg
     1201 accacgtcgt caggctcgcc ctcgccagca agcacgccat cgacgacctg gcgggccaag
     1261 ttgttggtca acgagccctc gccgatcagc gcgatcaccc gtgccacctg ctcgggggtg
     1321 atcggcagct cggcgagctc catgccgcgg gagttggcct gctgggacag gtacgacacc
     1381 caccacgacc gggcctcggc ggcgtgcgct ccggcttcca cggtggccgc gatgaggtcc
     1441 agcgcgccgg cgttgaccag atcgcgcaac tcgagatcag acaggcccca ctgggcctgt
     1501 atccgagctt ggcgctgcca gggcagctcg ggaagcgtcg cccgcagccg ctgcacccac
     1561 tcctcagcag gggcgatcgg taccaggtcg ggctccggga agtaccggta gtcctctgag
     1621 gtctccttgc gccgcccagg cgacgtggtg ccgttggcct cgtcgaagtg ccgggtctcc
     1681 tgcacgatgc ggccaccgga agccagcacc gcagcctgcc ggcacatctc gtggtgcacc
     1741 gcgcgctcca ccgaccgcag cgagttgacg ttcttggtct cggttcgggt accgaaggtg
     1801 gatgagccgc gtagcatcag tgagacattg gcgtcgcagc gcagtgaccc ttggtccatc
     1861 cgcacatcgg agacatccaa cgcccgcaac aggtcccgca gcgcggtgac gtaggcgcgg
     1921 gcgat
//
LOCUS       DATADN010000867         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_850236, whole genome shotgun sequence.
ACCESSION   DATADN010000867 DATADN010000000
VERSION     DATADN010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850236"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /gene="rpmC"
                     /locus_tag="VHH53_11220"
     CDS             <1..134
                     /gene="rpmC"
                     /locus_tag="VHH53_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX2290737.1"
                     /translation="QMDNNRRLRTVRHDIARIYTVMRERELGLSVSPDAIAGSEGAA"
     gene            179..445
                     /gene="rpsQ"
                     /locus_tag="VHH53_11225"
     CDS             179..445
                     /gene="rpsQ"
                     /locus_tag="VHH53_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX2290738.1"
                     /translation="MAEPQGRSKRKVREGYVVSDKMDKTVVVALEDRKKHPLYGKVMR
                     STTKVKAHDEANTAGVGDRVLLMETRPLSATKRWRLVQILEKAK"
     gene            519..887
                     /gene="rplN"
                     /locus_tag="VHH53_11230"
     CDS             519..887
                     /gene="rplN"
                     /locus_tag="VHH53_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX2290739.1"
                     /translation="MIQQESRLRVADNTGAKEILCIRVLGGSSRRYAGIGDIIVATVK
                     EAIPGANVKRGDVVKAVIVRTVKEKRRPDGSYIRFDENAAVLIKPDGEPRGTRIFGPV
                     GRELRDKRFMKIISLAPEVL"
     gene            986..1300
                     /gene="rplX"
                     /locus_tag="VHH53_11235"
     CDS             986..1300
                     /gene="rplX"
                     /locus_tag="VHH53_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003886882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX2290740.1"
                     /translation="MRVRKGDTVVVIAGKDKGAKGRVIQAYPDTGRVLVEGVNRIKKH
                     TRISRTQRGAQSGGIVTQEATIDVSNVMVVDSDGKPTRVGYRTDDEGKRVRVSRRSGK
                     DI"
     gene            1302..1865
                     /gene="rplE"
                     /locus_tag="VHH53_11240"
     CDS             1302..1865
                     /gene="rplE"
                     /locus_tag="VHH53_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX2290741.1"
                     /translation="MTTVEKKVPRLKSRYREEIAAALLEQFSYSNPMQVPAVVKVVVN
                     MGVGDAARDAKLMDGAVRDLSTITGQKPQVQRARKSVAQFKLREGMPIGAKVTLRGDR
                     MWEFLDRLLTIALPRIRDFRGLSPAQFDGNGNYTFGLNEQSMFHEINPDSIDRTRGMD
                     ITVVTSATTDEEGRALLRRLGFPFKES"
     gene            1865..2050
                     /locus_tag="VHH53_11245"
     CDS             1865..2050
                     /locus_tag="VHH53_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX2290742.1"
                     /translation="MAKKALIIKAARKPKFAVRGYTRCQRCGRPKAVYRKFGLCRICL
                     REMAHRGELPGVSKSSW"
     gene            2060..2458
                     /gene="rpsH"
                     /locus_tag="VHH53_11250"
     CDS             2060..2458
                     /gene="rpsH"
                     /locus_tag="VHH53_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX2290743.1"
                     /translation="MTMTDPIADMLTRLRNANSAYHDRVVMPHSKIKAHIAEILQREG
                     YISGFHTEEAPVGKSLVVDLKFGPNRERSIAGLRRVSKPGLRVYAKSTSLPKVLGGLG
                     VAIISTSTGLLTDRQAAKRGVGGEVLAFVW"
     gene            2475..3014
                     /gene="rplF"
                     /locus_tag="VHH53_11255"
     CDS             2475..3014
                     /gene="rplF"
                     /locus_tag="VHH53_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX2290744.1"
                     /translation="MSRIGKQPITVPSGVDVVIDGRTVTVRGPKGTLSHTVPEPITVQ
                     RAPDDTLLVERPDDERRSKSLHGLSRTLVNNMVVGVSQGYEKRMEIHGVGYRVVLKGS
                     DLEFSLGYSHPVPVAAPDGITFTVDSPTRFSVAGINKETVGEVAATIRKLRRPDPYKG
                     KGVRYAGEKIRRKVGKTGK"
     gene            3011..3427
                     /gene="rplR"
                     /locus_tag="VHH53_11260"
     CDS             3011..3427
                     /gene="rplR"
                     /locus_tag="VHH53_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX2290745.1"
                     /translation="MIMAEAATTTVTKRPKLGKDVSTQRRLARLRRHHRLRKRISGSP
                     ARPRLVVNRSSRHIAVQLVDDLAGHTLAAASSIEPDVRAMDGDKKAKAARVGELIAAR
                     AKAAGVGRVVFDRGGHDYHGRIAALADAAREAGLEF"
     gene            3511..>3634
                     /locus_tag="VHH53_11265"
     CDS             3511..>3634
                     /locus_tag="VHH53_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX2290746.1"
                     /translation="MPGRMRREGGGERSDRRDRREGGRGGATPPEKSPHLERVVA"
BASE COUNT          726 a         1077 c         1282 g          549 t
ORIGIN      
        1 ggcagatgga caacaaccgc cggctgcgca cggttcggca cgatatcgcc cgcatctaca
       61 ccgtgatgcg cgagcgtgag ttgggtctgt cggtgtctcc ggacgctatt gcgggaagtg
      121 agggtgcggc atgagccagg ccagcgagca gcagacccag accgaagggc aaagcaagat
      181 ggccgagccg caaggacgaa gcaagcgaaa ggtccgcgag ggttacgtcg tctcggacaa
      241 gatggacaag acggtcgtgg tggcgcttga ggaccgtaag aagcacccgc tgtacggcaa
      301 ggtcatgcgg tcgaccacca aggtgaaagc gcatgacgag gcgaacaccg cgggtgtcgg
      361 ggaccgggtg ctgttgatgg aaacccgccc gctgtcggcg accaaacgct ggcgactggt
      421 ccagattctc gagaaggcaa agtgatcggc tgccggtcag cgctgatgag ctgcctggtg
      481 ggtctgcgca agaccgtgcg gcgcgagaag gagttgacgt gatccagcag gagtcgcgac
      541 tgcgagtcgc cgacaacacg ggtgctaagg agatcttgtg catccgggtg ctcggcggca
      601 gctcgcgccg ctacgccggc atcggagaca tcatcgtggc gaccgtcaag gaagccattc
      661 ccggcgccaa cgtcaagcgg ggtgacgtgg tcaaggccgt catcgtgcgc accgtcaagg
      721 agaagcgccg cccggacggt tcctacatcc ggttcgacga gaacgctgcg gtactcatca
      781 agcccgacgg cgaaccacgc ggtacccgga tcttcggccc ggtgggtcgg gagctgcggg
      841 acaagagatt catgaaaatc atctcgttgg ccccggaggt gctgtagccc atgagcgagc
      901 gcagcgagcg aatcatcgga cgcagcgggc tagtgcctca gcgtctgatt tctgtggtgg
      961 ccgcacaccg gagggagtcg gctgcatgag agtgcgcaag ggtgacacgg ttgtggtcat
     1021 cgccggcaag gacaaaggcg ccaagggccg ggtgatccag gcctacccgg acaccggtcg
     1081 ggtgctcgtg gaaggcgtca accggatcaa gaagcacacc cggatcagcc gcacccagcg
     1141 tggtgcgcaa agtggcggga tcgtcacgca ggaagcgact atcgacgtct cgaacgtcat
     1201 ggtcgtcgac tcggacggca aaccgaccag ggtcggctac cgaactgatg acgaaggcaa
     1261 gcgcgtgcgc gtgtcgcggc gcagcggcaa ggacatctga gatgacgacc gtagagaaaa
     1321 aggtacccag gctcaagagc cggtaccgcg aggagatcgc cgccgcactg cttgagcagt
     1381 tcagctactc caatccgatg caggtacctg cagtggtgaa ggttgtggtg aacatgggcg
     1441 tcggcgacgc cgcccgggat gccaagctga tggacggtgc cgtgcgcgac ctatcgacga
     1501 tcacgggcca gaagccgcag gtgcagcggg cccgtaagtc cgtcgcgcag ttcaaattgc
     1561 gcgagggcat gccgatcggc gccaaggtga ctctgcgcgg cgatcgaatg tgggagttcc
     1621 tggaccgctt gctgacgatc gcgctgcccc gtatcaggga cttccgtggt ctgtcgccgg
     1681 cccagtttga cggtaacggc aattacacgt tcggactcaa cgagcagtcg atgtttcacg
     1741 aaatcaatcc ggacagcatc gaccgcacgc gcggcatgga tatcaccgtg gtcacctcgg
     1801 cgaccaccga cgaggagggc cgggcgctgc tgcgacgtct gggcttcccc ttcaaggaga
     1861 gctgatggcg aagaaggcgc tgatcatcaa agccgcgcgc aagcccaagt tcgcggtgcg
     1921 gggctatacc cgctgccagc gctgcgggcg tcccaaggcg gtgtaccgga agttcgggct
     1981 atgccggatc tgcctgcgcg agatggctca ccgtggcgag ctgcccggcg tcagtaagag
     2041 cagctggtag gagatcgcta tgacgatgac cgacccgatc gcagacatgc tgacccgtct
     2101 gcgcaacgcg aactcggctt atcatgaccg cgtcgtgatg ccgcactcca aaatcaaggc
     2161 gcatatcgcc gagatcctcc agcgggaggg ttacatctcc ggcttccaca ccgaggaagc
     2221 gccggtaggc aagtccttgg tggtcgattt gaagttcggc ccaaaccggg agcgcagcat
     2281 cgcgggcctg cgccgggtct ccaaacccgg cttgcgggtg tatgcgaagt cgacctcgct
     2341 gcccaaggtg ctcggtggcc tgggcgtggc gatcatctcc acgtccaccg ggttgctcac
     2401 cgaccggcaa gccgctaagc gaggcgtggg cggggaagtc ctcgccttcg tctggtgaga
     2461 ggggagcaga cgagatgtcg cggatcggaa agcagccgat caccgtgcct tccggggtcg
     2521 atgtggtcat cgacgggcgc accgtgacgg tgcgcggacc caagggcacc ctgtcacaca
     2581 ccgtgccgga gccgatcacc gtacagcggg cgccggacga caccctgctg gtcgagcgac
     2641 ccgacgacga gcggcgcagc aagtcactgc acgggctgtc ccggaccctg gtgaacaaca
     2701 tggtcgtcgg cgtcagtcag ggttatgaga agcggatgga gatccacggc gtcggttatc
     2761 gcgtggtgct caagggctcg gatctggagt tctccctggg ctacagccat ccagtcccgg
     2821 tggcagcgcc cgatgggatc accttcacgg tcgacagtcc gacgcggttc tccgtggcgg
     2881 gtatcaacaa ggagacagtc ggcgaggttg cggcgaccat ccgcaagctg cgtcgccctg
     2941 atccgtacaa gggcaagggc gtgcgctatg cgggcgagaa gatccggcgc aaggtcggga
     3001 agacaggtaa gtgatcatgg ctgaggcggc aacaaccacc gtgacgaaac gtcccaagct
     3061 tggcaaggac gtctcgaccc aacgccggtt ggccaggttg cggcggcacc accggcttcg
     3121 taagaggatc agcggttcgc cggcgcgccc gcgcctggtg gtcaaccgct cgtcgcggca
     3181 catcgccgtg cagttggtgg atgatctggc cgggcatacc ctggcggcgg catccagcat
     3241 cgagccggac gttcgcgcca tggacgggga caagaaggcc aaggcggcca gggtcgggga
     3301 gctgatagcg gcccgagcca aggctgccgg cgtgggccgg gtggtcttcg accgtggcgg
     3361 ccacgactat cacggccgga tcgcggcgtt ggccgacgcc gcccgcgagg ccggcttgga
     3421 attctgactg atggagaaaa ccggactgca cagcaggtcg tacacgaggc agcaacaaca
     3481 gcagcacgcg agcaatggaa ggaacgcctg atgcctggac gcatgcggcg ggaaggcgga
     3541 ggcgagcgta gcgatcgtcg ggaccgccgc gaagggggcc gcggaggggc gaccccaccg
     3601 gagaagagcc cgcacctgga gcgcgtggtc gcga
//
LOCUS       DATADN010000868         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_850470, whole genome shotgun sequence.
ACCESSION   DATADN010000868 DATADN010000000
VERSION     DATADN010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850470"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VHH53_11270"
     CDS             <1..286
                     /locus_tag="VHH53_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290747.1"
                     /translation="PDGTVRVVDAMPLRGEATDVVRVVEGRSGRVPMVMELVLRFDYG
                     SVSPWVRQVNGQWSAIAGPDAAWLYSPVAVEHRDGWTSSRWRRGNRCRSC"
     gene            253..1542
                     /locus_tag="VHH53_11275"
     CDS             253..1542
                     /locus_tag="VHH53_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX2290748.1"
                     /translation="MAAGQQVPFVLTHTASHVPAPKPVDGLQALEGTEWFWTDWIGRC
                     NYQGEWAQPVRRSLVTLKALTYAPTGGIVAAATTSLPEQLGGSRNWDYRYCWLRDATF
                     TLQALLGTGYVDEAKAWRDWLLRAVAGDPADLRIMYGLDGTRRIPEYTLDWLRGYEGS
                     APVRVGNAAAEQYQLDVWGEVLEGLHLGREAGLAPSEDAWDLQRALLDYLEGHWTDPD
                     NSLWEVRGARRHFVHSKVLAWTGVDRAVQAVERQGLDGPVARWRALRDRIHRDVCERG
                     YDAQRGTFTQFYGAHGLDAALLLIPRVGFLPWDDERVVGTVDAIQRELCEDGFVLRYR
                     TDSDGGVDGLPGEEGAFLLCSFWLADALHGIGRTQQARALFERLLGLRNDLGLLSEEY
                     DTRAGRQLGNTPQAFSHVGLVNTARHLRGSSTSTEVS"
     gene            1542..>2316
                     /locus_tag="VHH53_11280"
     CDS             1542..>2316
                     /locus_tag="VHH53_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2290749.1"
                     /translation="MRALTVVPGRAGSLAVADVAEPEPGEDELLVDGLAVGVCGTDRE
                     IAAGEYGWAPPGRDGLVLGHESLGRVRHAPGGSGFTAGDLVAGVVRRPDPVPCGACAH
                     GQFDMCRNGRYTERGIKQLDGYASQRWRVEPGYAVRLDPRLAEVGVLMEPASVVAKAW
                     DQIEWIGQRSWFDPQRVLITGAGSIGLLAALLAVQRGLDTHVLDVVTHGRKPELVRAL
                     GATYHYDGVASATRAAEPDVVIEATGVASVVFDALAATAP"
BASE COUNT          314 a          763 c          883 g          356 t
ORIGIN      
        1 gccggacggc acggtccggg tggtcgacgc gatgccgctg cgcggcgagg ccaccgatgt
       61 ggtccgcgta gtcgagggcc ggtcggggcg ggttccgatg gtgatggagc tcgtgctgcg
      121 cttcgactac ggctcggtgt cgccgtgggt gcgccaggtc aacggccagt ggtcggcgat
      181 cgcgggaccc gatgcggcct ggttgtacag cccggttgcc gtcgagcaca gggacggctg
      241 gacgagctcg aggtggcggc ggggcaacag gtgccgttcg tgctgaccca taccgcgtcc
      301 cacgtgccgg ctccgaagcc ggtcgacggc ctgcaggcgc tggagggcac cgagtggttc
      361 tggacggact ggatcggacg ctgcaactac cagggcgaat gggcgcaacc agtgcgccgc
      421 tcactggtca cgctcaaagc cctcacctac gccccgaccg gtggcatcgt ggcggcggcg
      481 accacctcgt tgcctgaaca gctgggcggc agccgtaact gggattaccg gtactgctgg
      541 ctgcgcgacg ccaccttcac cctgcaagca ttgctcggca ccggctatgt cgacgaggcg
      601 aaggcctggc gggactggct gttgcgggcg gtggccggtg accccgccga cctgcggatc
      661 atgtacgggc tggacggcac ccgccggatc ccggagtaca ccctggactg gctgcgtggt
      721 tacgagggct cagcgccggt gcgggtcggc aacgccgccg ccgagcagta ccaactcgac
      781 gtgtggggcg aagttctcga agggctgcac ctgggccgcg aggctgggtt ggcaccctcg
      841 gaagacgcgt gggacctgca gcgggcgctg ctggactacc tggagggcca ctggaccgat
      901 ccggacaaca gcctgtggga ggttcgtggg gcacggcggc acttcgtgca ctccaaggtg
      961 ctggcgtgga ccggcgtcga ccgcgcagtg caagccgtcg agcggcaggg cctggacgga
     1021 ccggtggcgc gctggcgggc gctgcgggac cggatccacc gcgacgtgtg cgagcgcggc
     1081 tacgacgccc agcgcggcac cttcacccag ttctacggcg cccacggact ggacgcagca
     1141 ctgctgctga taccccgagt ggggttcctg ccctgggacg atgagcgggt tgtgggtacc
     1201 gtcgatgcga tccagcgaga gctgtgcgag gacggcttcg tcctgcgata tcgcaccgac
     1261 tccgatggtg gggtcgacgg cttgccgggc gaggagggcg cgttcctact gtgctcgttc
     1321 tggctggcgg acgcgctgca cgggatcggg cggacgcagc aggcgcgggc gctgttcgag
     1381 cgactgctcg gattgcgcaa cgacttgggt ctgctcagcg aggagtacga cacgcgggcc
     1441 ggccgccagc tgggaaacac cccgcaggcc ttcagccacg tcggactggt caacaccgcc
     1501 cgccacctcc gtggctcttc cacctcgacc gaggtgtcat gatgcgggcg ctgaccgttg
     1561 tgccgggccg agctgggtcg ctggcggtgg ccgacgtggc tgagcccgag cccggcgagg
     1621 acgagctgct cgtcgacggt ctcgcggtcg gcgtgtgcgg tacggaccgc gagattgccg
     1681 cgggggagta cggctgggcg ccgccgggcc gcgatgggct cgtcctgggc cacgagtcgc
     1741 tgggccgggt gcgccacgca cccggtggct ctggcttcac ggccggggac ctcgtggcgg
     1801 gcgtggtgcg ccggcccgat ccggttccct gtggcgcctg cgcacacggg cagttcgaca
     1861 tgtgccgcaa cggtcgctac accgagcgcg gcatcaagca gctcgacggc tacgccagcc
     1921 agcggtggcg ggtcgagcct ggctacgcgg tccggcttga cccgcgcctg gccgaggtcg
     1981 gcgtgctcat ggagccagcc agcgtcgtgg ccaaggcgtg ggatcagatc gagtggatcg
     2041 gtcagcggtc gtggttcgat ccgcagcgcg tcctgatcac cggcgcaggg tcgatcggac
     2101 tgctcgcggc gctgctcgcc gtgcagcgcg gcctggacac ccacgtgctc gacgtggtca
     2161 cccacggccg caagccggaa cttgtgcgcg cgctcggtgc gacctatcac tacgacggcg
     2221 tggcttcggc cacgcgggcg gccgagcccg acgtcgtcat cgaggccacc ggggtggcct
     2281 ccgtggtctt cgacgcgctc gccgccaccg cgccct
//
LOCUS       DATADN010000869         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_564284, whole genome shotgun sequence.
ACCESSION   DATADN010000869 DATADN010000000
VERSION     DATADN010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564284"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VHH53_11285"
     CDS             <1..358
                     /locus_tag="VHH53_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2290750.1"
                     /translation="EAPIHTPVFVVTHTKRDPRERPGGTTFHFVNDGIESALLQAREA
                     AGDRDVRIAGGAETIQQYLDSGLIDEFSITLAPVLFGTGIRLFERVDPARLALDQVHT
                     DTSSRLTHLTYTVGKR"
     gene            491..1495
                     /locus_tag="VHH53_11290"
     CDS             491..1495
                     /locus_tag="VHH53_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX2290751.1"
                     /translation="MSHRNAPLSFEGRLRLVQRCEQRPISHVAAEMGISRACASKWVN
                     RWRRHGELGLHDRPSTPHHSPDATPAWVIERIETWRREKKWSANRITHELAELDFRIN
                     RRTVTRHLTRLGLGQRRFIDPSGHSNRKPGKIIARWPGHMAHLDVKKVGRIPDGGGWR
                     IHGRNCAQKRAVDRAKTAGAKAGYLYLHSIVDGFSRLAYTEPLDDEKGTTAAAFLARA
                     KVWFAAHGITHIHRIVTDNGACYRSNDFARIVGHRTRHQKTKPYTPRHNGKAERYQRI
                     MTEELLYAREYASEDERSQAIAIWNVHYNYHRPHSAAGGQPPASRLKTRVTNVRPSYT
                     "
BASE COUNT          304 a          599 c          478 g          221 t
ORIGIN      
        1 cgaggcgccg atccacaccc cggtcttcgt cgtgacgcac accaagcgtg accccaggga
       61 gcggccgggc ggtaccacgt tccacttcgt caacgacggc atcgagagcg cgctgctcca
      121 ggcccgcgag gccgccggcg accgcgacgt ccgcatcgcc ggcggcgccg agacgatcca
      181 gcagtacctg gacagcggcc tgatcgacga gttctcgatt accctcgcgc ccgtactgtt
      241 cggcaccggc atccgcctgt tcgagcgcgt ggatcccgcc cgcctcgccc tcgaccaggt
      301 ccacacggac acatcatccc ggctgaccca cctgacctac accgtcggga aacgctagcc
      361 cgcgtccccg cagcgtgcgg tgccggcgtg tccccgcagg gcgaggtgcc ggtcgcgtcg
      421 cgggacttgc gcagggtgga aagtgggccg ttgccggggc ggatcgagcc agcgaaagga
      481 tctgcccctc atgtcccacc gtaatgcccc gttgtcgttc gaaggccgac tgcgtctggt
      541 gcagcgttgt gagcagcgcc cgatcagtca tgtcgccgct gagatgggaa tctcgcgggc
      601 gtgcgcgtcg aagtgggtca accggtggcg ccgccatggt gagctcggcc tgcacgaccg
      661 gccctcaacc ccacaccaca gtccggacgc gacccctgca tgggtcattg agcggatcga
      721 gacctggcgg cgggagaaga agtggtcggc caaccggatc acccacgagc tggccgagct
      781 ggacttccgc atcaaccgcc gcaccgtcac ccgccacctg acccgactcg ggcttggtca
      841 gcgcaggttt atcgacccca gcggtcacag caaccgcaag cccggcaaga tcatcgcccg
      901 ctggccgggt cacatggcac atctggacgt gaagaaggtc ggccggatcc ccgacggcgg
      961 cgggtggcgc atccacggcc gcaactgcgc ccagaagcgg gcagtcgacc gcgccaaaac
     1021 cgcaggcgcc aaggccggtt acctctacct gcactccatc gtggacgggt tctcccgact
     1081 ggcctacacc gaaccactcg acgacgagaa gggcacgacc gcagccgcat tcctcgcccg
     1141 agccaaggtc tggttcgccg cccacggcat cacccacatc caccgcatcg tcaccgacaa
     1201 cggcgcctgc taccgctcca acgacttcgc ccgcatcgtc ggccatcgca cccgccacca
     1261 gaagaccaag ccctacacgc cacgccacaa cggcaaggcc gagcgctacc agaggatcat
     1321 gaccgaagaa ctcctctacg cccgcgaata cgccagcgag gacgaacgct cacaagccat
     1381 cgccatttgg aacgtccact acaactacca tcgaccccac agcgccgcgg gcggccaacc
     1441 gccagcatcc cgactcaaga cccgcgtcac caacgtccgg ccctcataca cctagaagat
     1501 gaatttgaac cctatgggcg tctcgggcta ggcgctggag gagcagctgt tcgagctgtg
     1561 cgggcgggcg gttagaccca ctccaccctg gggatcttcg ac
//
LOCUS       DATADN010000870         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1171137, whole genome shotgun sequence.
ACCESSION   DATADN010000870 DATADN010000000
VERSION     DATADN010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171137"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VHH53_11295"
     CDS             complement(<1..1346)
                     /locus_tag="VHH53_11295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2290752.1"
                     /translation="MTRTTERPAPQRDCADVGTPSEPSSTLLQRVILPRRADPMAVRA
                     LYVDEQSATARRIWPPAGVTAAANPHDVDLEVTLANPNARRVRALSRTSVRVPEQTEV
                     SFAAYFNAFPASYWRRWSALRTLHLRLDVEGAGRIDVYRSKADATPIHTHGELVNGAA
                     GRRELDIELDLTPFEDGGWYWFDISTEDSELVVHSGGWHAPVEAPGRAAVTIGMPTFN
                     RPTDCVATLRAIGEDDLVRSIVTAVVIPDQGANKVRDQDGFAQAQAVLGDRLRLIDQP
                     NIGGSGGYARVMYEALENTDCEQILYMDDDIVLEPDSILRAVAFSRFARRPMLVGGQM
                     LQAQARSRLHAWGEIVDRQRMRWNKAPGTEYDHDFAHHPLRQTAWMHRRTDVDYNAWW
                     MCLIPRTIAEDIGLPLPLFIKWDDVEYGLRARAAGYPTATVPGVAIWHMSFADKDDA"
     gene            complement(1350..>1819)
                     /locus_tag="VHH53_11300"
     CDS             complement(1350..>1819)
                     /locus_tag="VHH53_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX2290753.1"
                     /translation="DYAEGELGWRTLDFEQEVVTTGDFQGTPVMNYADLDVPYTRIHE
                     FRHFHPERDYRTDKTVIFREYSRFAQRGDEPYYPINTAEDRVKLERYRELARKETANH
                     NVLFGGRLGTYKYLDMHMAIGSALSMYDNRIAPYFTEACPLDGPASFTASLED"
BASE COUNT          304 a          605 c          608 g          302 t
ORIGIN      
        1 gcgtcgtcct tgtcggcaaa ggacatgtgc cagatcgcca caccgggcac tgtcgcggtg
       61 ggatagcccg cggcgcgggc gcgcagcccg tactcgacgt cgtcccactt gatgaacagc
      121 ggcagcggca gcccgatgtc ctcagcgatg gtgcgcggga tcaggcacat ccaccaggcg
      181 ttgtagtcca cgtcggtgcg ccggtgcatc caggcggtct ggcgcagcgg gtggtgggcg
      241 aagtcgtggt cgtactcggt gcccggcgcc ttgttccacc gcatccgttg ccggtcgacg
      301 atctcgcccc aggcgtgcag ccgggagcgg gcctgggcct gcagcatctg cccgccgacc
      361 agcatcgggc ggcgcgcgaa gcgagagaac gccaccgcgc gcaggatcga gtcgggctct
      421 aagacgatgt cgtcgtccat gtagaggatc tgctcgcagt cggtgttctc cagcgcctcg
      481 tacatcaccc gggcgtaacc gccggaacca ccgatgttgg gctggtcgat gagccgcagc
      541 cggtcaccga gcaccgcctg cgcctgggcg aagccgtcct ggtcacggac cttgttggcg
      601 ccctgatcgg gaatcaccac cgcggtcaca atcgaacgga ccaggtcgtc ctccccgatc
      661 gcgcgcaggg tggccacgca gtcggtggga cggttgaagg tcggcattcc gatggtcacc
      721 gcggcccgac ccggtgcctc gaccggcgcg tgccagccac cggagtgcac gacgagctcc
      781 gagtcctcgg tgctgatgtc gaaccagtac cacccgccgt cctcgaacgg cgtcagatcc
      841 agctcgatgt ccagctcgcg acgacccgca gcaccgttga ccagctcacc atgggtgtgg
      901 atcggggtgg cgtcagcttt ggatcggtag acgtcgatgc ggcccgcgcc ctcgacgtcc
      961 agccgcaggt gcaacgtgcg cagcgcgctc cagcgccgcc agtagctggc cgggaaagcg
     1021 ttgaaatacg ccgcgaagga cacctcggtc tgctcgggca cccgcacgct agtcctggac
     1081 agcgcacgca cccgccgcgc gttggggttg gcgagggtga cctcgaggtc gacgtcatgg
     1141 ggattggccg cggcagtaac cccggcgggg ggccagatcc gccgcgcggt ggccgactgc
     1201 tcatcgacgt acagcgcgcg gacggccatc gggtcggcgc gccgcggcag gatcacccgc
     1261 tgcagcagcg tcgacgaagg ctcggaaggc gtccccacgt ccgcacaatc gcgttgcggt
     1321 gctgggcgct cggtggttct tgtcaagcgt cagtcctcca aagacgctgt aaaagaggct
     1381 gggccgtcaa gtgggcaggc ctcagtgaag tagggcgcga tccggttgtc gtacatcgac
     1441 agcgccgagc cgatcgccat gtgcatgtcc aggtatttgt aggtaccaag gcgcccaccg
     1501 aagagcacgt tgtggttggc cgtctccttg cgggccagct cccggtagcg ctccaacttc
     1561 acccgatcct ccgcggtgtt gatcggataa tagggctcgt caccacgttg cgcgaaccgc
     1621 gagtactccc gaaagatcac cgtcttgtcg gtgcggtagt cccgctccgg atgaaaatgc
     1681 cggaactcgt gaatccgggt gtagggcacg tccaggtcgg cgtagttcat caccggcgtc
     1741 ccctgaaaat caccagtagt cacgacctct tgctcgaaat ccagagttcg ccagcccagc
     1801 tcgccctcag cgtaatcga
//
LOCUS       DATADN010000871         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_711204, whole genome shotgun sequence.
ACCESSION   DATADN010000871 DATADN010000000
VERSION     DATADN010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711204"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VHH53_11305"
     CDS             <1..285
                     /locus_tag="VHH53_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX2290754.1"
                     /translation="GGPRVDFAVEVERHVEAILAELKPGRELHTNVEYYAGVVMELCG
                     LPREMFTPTFAAARAVGWSANILEQAEDTKIIRPVARYVGPGAPVAVPAV"
     gene            528..767
                     /locus_tag="VHH53_11310"
     CDS             528..767
                     /locus_tag="VHH53_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290755.1"
                     /translation="MSQIIRRLGVAPRYRGSTGGGTCPDIFELADGNFAVIGTDVTDS
                     LDAQLPDDAARADYERIVVITRETLVKAKKDIPDV"
     gene            926..2194
                     /locus_tag="VHH53_11315"
     CDS             926..2194
                     /locus_tag="VHH53_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2290756.1"
                     /translation="MARIAGRFSRVEPRATARAYLLGLLSRAERKNCWQLAEQAGDAR
                     PGPMQRLLRCARWDADAVRDDIRAYAVEHLGAQGAVLIVDETVFVKKGRASAGVQRQY
                     TGTAGRIENSQVGVFLAYATSRGRTLIDRRLYLPEQSWCSDPGRRQAAGIPETVQFQT
                     KPRLAWEMIAAALDAGVEAPWVTGDEAYGQDPQLRAALEARGTGYVMAVACSTRVRIN
                     SGRTTMRADTVAERLPASAWQRHSAGNGAKGPRYYDWAWIHIGTDGHRHLLVRRNRTI
                     GELAFYLCWSPATVTLAELVRVAGVRWSVEECFQAAKGQVGLDHYQVRNWTSWHRHIT
                     LAMLALAFLTALAVDAAPDRPIDPHHPARGSDPITLTVPEIRRLLVVALIPPTVTAAR
                     LLHWSAWRRRHQATARRSHYRQRSTDQLTG"
BASE COUNT          400 a          825 c          782 g          376 t
ORIGIN      
        1 ggcggtccgc gggtggactt cgccgtggag gtcgagcgcc acgtcgaggc gatcctggcg
       61 gagctgaagc ccggccgtga gctccacacc aacgtcgagt actacgcggg cgtggtcatg
      121 gaactgtgcg gcctgcctcg cgagatgttc acgccgacct tcgccgccgc acgagcggtg
      181 ggctggagcg ccaacatcct ggaacaggcg gaggatacga agatcatccg gccggtggcg
      241 cggtatgtgg ggccgggggc gccggtcgcg gtgccggctg tctaaaggcc gcaattgttt
      301 tatacgggct gatgatcgat tcttcactgt cttccgtcac tcttggggat gttcctcttg
      361 gccttgagag gtgccacccc cgcgggtgat aaaaagggcc acaaaggcgc gttgcgccct
      421 tggattgtgc gccggtcaga gctcaggcct gcgaagatgc ccctcgcgcc gcatcgcgcg
      481 gctacgggcg taggcgctcg tgccttgaga acagagggaa ttcaacagtg agtcagatca
      541 tccgccgcct cggtgtcgct ccccgctatc gaggcagtac tggtggtggg acatgtcccg
      601 acatttttga attggctgac gggaatttcg ctgtcatcgg cacagatgtg accgactctc
      661 tggatgccca gcttcccgac gatgccgcac gcgccgacta cgaacgcatc gtcgtgatca
      721 ctcgggaaac ccttgtcaag gcgaagaagg acatcccgga cgtctagtac tccagtgtga
      781 cttcgcgatc ttgggtcgga cccgcgtgag aactggaacg gccaccgcgt gatcatcaag
      841 ggttgtgtgg actcatgacg atcgtgcggt ggccgcaggt cacagcatag acggcgcccg
      901 atggcgggcg atgttcgagc aggccatggc ccggatcgcg ggccggttca gccgagttga
      961 accccgggct accgcccgcg cctacctgct cggactgctg tcgcgtgccg agcggaagaa
     1021 ctgctggcag ctggccgaac aggcaggcga tgcgcggccg ggcccgatgc agcggctgct
     1081 gcgctgcgcc cgctgggacg ccgacgccgt ccgcgacgac atccgcgcct acgccgtcga
     1141 acacctcggc gcccagggcg cggtgctgat cgtggacgag accgtcttcg tgaagaaggg
     1201 ccgggcttcg gcgggcgtgc agcgccagta caccggcacc gccgggcgca tcgagaactc
     1261 ccaggtcggc gtcttcctcg cctacgccac cagccgagga cggacactga tcgaccgccg
     1321 gctctacctg cccgaacagt cctggtgctc cgatcccgga cgccgccagg cggccgggat
     1381 acccgagact gtgcagtttc agaccaagcc ccgcctggcc tgggagatga tcgccgccgc
     1441 gctggacgcc ggggtggagg caccgtgggt gaccggcgac gaagcctacg ggcaggaccc
     1501 tcagcttcgt gccgccctgg aggcacgcgg gaccggctac gtgatggccg tcgcctgctc
     1561 cacccgggta cggatcaact caggccgcac caccatgcgc gcggacaccg tcgccgaacg
     1621 cctgcccgcc agcgcctggc agcggcacag cgcaggcaac ggcgcgaagg gcccgcgcta
     1681 ctacgactgg gcctggatcc acatcggcac cgacggacac cgccacctgc tcgtccgccg
     1741 caaccgcacc attggtgaac tggccttcta cctgtgctgg tcacctgcca cggtcaccct
     1801 tgcggaactc gtccgggtcg ccggcgtccg ctggagcgtc gaggagtgct tccaggccgc
     1861 caagggccag gtcggactcg atcactacca ggtccgcaac tggacctcgt ggcaccgcca
     1921 catcacgctc gccatgctcg ccctggcctt cctgaccgcc ctcgcagtcg acgcagcccc
     1981 cgaccggccc atcgatcccc accaccccgc ccgcggcagc gacccgatca cgctgaccgt
     2041 ccccgagatc cgccgcctac tcgtcgtcgc gctcatcccg ccgaccgtga cagcggccag
     2101 gctcctgcac tggtctgcct ggcgccgacg ccaccaagca acagcccgcc gcagccacta
     2161 ccgacaacgg tcaactgacc aactcaccgg atagatcacg aaaccgcact ggagtactaa
     2221 atgccaacag ttggcccgtg agctcacacg tggctgcgga ccagcaggag tgccacgtcg
     2281 tcgtcgggtt ccggacgcag ggcgctgagg agactgtcgc tggtgcgttg caggctggga
     2341 cccgcgctct gcagcaggcg ggtcagcgtg gtgaggccgg tgt
//
LOCUS       DATADN010000872         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1021108, whole genome shotgun sequence.
ACCESSION   DATADN010000872 DATADN010000000
VERSION     DATADN010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021108"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1818
                     /locus_tag="VHH53_11320"
     CDS             163..1818
                     /locus_tag="VHH53_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator"
                     /protein_id="HEX2290757.1"
                     /translation="MTAPTEPTIVDVEQMTRAQRAALPLSTDVAQALAEQHGVCIQPL
                     AMRRIDVSTGRVEVVPVPCGSTREDQCRPCAEKARRLRMVQCREGWHLDAEPLIDRAT
                     PSEDHKALMATRADLLTAYTECKTTGDELSCEQITESVAELDIELRAAGVRGRLAPLD
                     PLPKPVKRSTRRRQDAPDLPRRPVKRRTVGRVFAGRYRPSTFLTLTLDSYGRVDNDGG
                     AVDPDEYDYRRAARDAIHFPALLDRFWQNTRRCVGWDVQYFGTVEPQKRGAPHFHAAI
                     RGAIPRAELRAITAATYHQVWWPAHDQLLYTDERLPVWDTRAKAFVDPDTGTPLPTWH
                     EACDELTEPAHVVRFGAQVHVKGILGGTEEAGRHVGYITKYLTKNISQAAGLDQHATD
                     AQRDHARRLHAELQITPCSPRCPMWLRYGIQPKGARYSMTPGRCKGKAHKPEYLGIAG
                     RRILVSRKWSNKSLDDHRAERSEFVRQLLEAVGIQPSHGPRDGPYQWERPAPTDPDVP
                     PRPVLLLQAVAERQRWKAEYTAAQLATGAAPPGNWSATDDQAA"
     gene            1839..2084
                     /locus_tag="VHH53_11325"
     CDS             1839..2084
                     /locus_tag="VHH53_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2290758.1"
                     /translation="MDNVTSLWALDVPPEDPVSLNDFTHADIYTVRQAAARLGISTGL
                     AYELIRAGEIPAKRLGRRWVVPRELFHTWLNEMPKGA"
BASE COUNT          406 a          755 c          624 g          308 t
ORIGIN      
        1 ctcagtaacc gggctattga gccgcgctgc ctcggtcctg gtctatagcg gtttaccggt
       61 cactgattgt ggtgtgggtg ctgatcttgc acccggtgcc accacgatta tcccgctcac
      121 gatgctgcgg cacgccgcca ccatccagga ggcgagggcc tgatgactgc ccccaccgag
      181 cccaccatcg ttgatgtcga gcagatgacc cgggcgcagc gggccgctct accgctgagc
      241 actgatgtcg cccaggccct ggccgaacag cacggcgtct gcatccagcc acttgccatg
      301 cgccggatcg atgtctccac cggccgcgtc gaggtcgtgc ccgtgccctg cgggtccact
      361 cgtgaggatc agtgccgccc gtgtgcggag aaggcccgcc ggttgcggat ggtccagtgc
      421 cgcgaaggct ggcacctcga tgcggagcca ctgatcgacc gggccacacc cagtgaggac
      481 cacaaggcac tgatggccac gagggccgat ctgctcaccg cctacaccga atgcaaaacc
      541 accggcgacg aactgagctg tgagcagatc accgagtcgg tcgccgagct cgacatcgaa
      601 ctccgcgccg ccggagtgcg cggccgactc gccccgctcg atccattacc caagccggtc
      661 aagcgctcca cccgacgccg tcaggatgcc ccggacctgc ctcgccgacc agtcaagaga
      721 cgaacggtag gacgagtgtt cgccgggcgc taccggcctt ccaccttcct gaccctcacc
      781 ctcgactcct acggccgcgt cgacaatgac gggggagccg tcgatccgga tgagtatgac
      841 taccggcggg ccgctcggga cgcgatccac ttcccggccc tgctggatcg gttctggcag
      901 aacacccggc gctgtgtcgg ctgggatgtg cagtacttcg gcaccgtcga acctcaaaaa
      961 cgcggcgcac cgcatttcca tgccgccatc cggggagcca tcccccgggc cgagctgcga
     1021 gccatcacgg cagcgaccta ccaccaagtc tggtggcccg cgcacgatca gctcctctat
     1081 accgacgagc ggctgccggt ctgggacacc cgggcaaaag ccttcgtcga ccccgacacg
     1141 ggcacaccgc tgccaacctg gcacgaagcc tgtgacgagc tgaccgaacc cgcccacgtc
     1201 gtccgattcg gggcacaagt gcacgtcaag ggcatcctgg gcgggactga ggaggctggt
     1261 cggcacgtcg gctacatcac gaaatatttg acgaagaaca tcagccaggc tgccgggctc
     1321 gatcagcacg ccaccgacgc ccaacgggat cacgcccggc gtctgcacgc cgaactccag
     1381 atcaccccct gctcgccccg gtgcccgatg tggctgcgct acggaatcca gcccaagggc
     1441 gcccggtact ccatgacccc gggacgctgc aagggcaagg cccacaagcc cgaatacctc
     1501 ggcatcgccg gacgccgaat cctcgtctcc cggaaatggt ccaacaagtc attggatgac
     1561 caccgggcag agcgcagcga attcgttcgg caactcctcg aagccgtcgg catccaaccc
     1621 agccacggcc cgcgagacgg gccctaccaa tgggagcgcc cagcaccaac agatcccgac
     1681 gtgccaccac gacccgtcct tctgctgcag gcagtcgctg aacgacagcg atggaaagcc
     1741 gaatacaccg ccgcccaact tgccaccggc gccgcaccac caggcaactg gtcggcaact
     1801 gacgatcagg ccgcatgaac gggacaggag gacaacccat ggacaacgtg acctcgctgt
     1861 gggcccttga cgttccaccc gaggacccgg tgtcacttaa cgacttcact cacgcggaca
     1921 tctacaccgt gcggcaagcc gccgcccggc tgggcatcag caccgggctg gcctacgagc
     1981 tgatccgggc tggcgagatc ccggctaagc ggctgggccg acgctgggtg gtgccccgag
     2041 aactgtttca cacctggctc aacgagatgc caaagggagc atgacatgtc att
//
LOCUS       DATADN010000873         1854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_418412, whole genome shotgun sequence.
ACCESSION   DATADN010000873 DATADN010000000
VERSION     DATADN010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418412"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VHH53_11330"
     CDS             <1..321
                     /locus_tag="VHH53_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290759.1"
                     /translation="EAVPPPPERVVAYIDGYGNLKTTWSQPPTPSGTRVRVHVGDRSV
                     EATVSDGTFEVPAGETSFAPGSSGWRTSNGDVAWYELFARGASAAALLGHPAAGAEVK
                     VSPL"
     gene            complement(583..>1854)
                     /locus_tag="VHH53_11335"
     CDS             complement(583..>1854)
                     /locus_tag="VHH53_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2290760.1"
                     /translation="VLVERYGFLGGNATVALVMPLMSFHNEKKQAVPGENTRLLPTDH
                     GQGDPVVAGVLWQLLQRLTQRGGAISPSASTGYTVPFDPEQFKLAALDMLDQAGVRLL
                     FHAFASGVSSDSRLPRVVFETKSGPVVIDAQVVVDCTGDGDVAAASGAAFEVGRTEDG
                     LVQPMTLMFRMVDFLQSRFADYVRVHPDQWRGVHGLWDLIQQATEAGDLELPREDLLF
                     FATPHPHELSVNSTRVTRALGTSVWDLSRAEFVARRQLDQISRFLRDRVPGFENSYVA
                     QSGVQVGVRETRRVLGDYQLTGNDILAAKKFPDAIARGAYPVDIHNPEGTGTVLKRVP
                     KGQAYDIPLRCLLPRGVDRLIVAGRCISGTHVAHSSYRVMPIAMATGHAAGVCAALAT
                     RTDKLPRDVPAEEVQQELRAQGASLRAGVGE"
BASE COUNT          329 a          571 c          615 g          339 t
ORIGIN      
        1 gaggcggtac cgccgccacc ggagcgggtt gtggcgtaca tcgacggcta cggcaacctg
       61 aagaccactt ggtcacagcc gccgacgccg tccggcaccc gggtgcgggt acacgtcggc
      121 gaccgtagcg ttgaggcgac cgtgagcgat ggcacgttcg aggtgccagc cggagagacg
      181 tcgttcgcac ccggaagctc cgggtggcga acctccaacg gggacgtcgc ctggtatgag
      241 ttgttcgccc gcggcgccag cgccgccgcg ttgctcggcc atccggcagc gggcgccgag
      301 gtcaaggtca gcccactctg aggcgggctg ggacgccgct tagcgccttc tcgcgccgtg
      361 atctccccgc atagtttttg atatgggggt ggaggaccac gcacgtggtc tgtggtttgg
      421 gacttggtga ttagctgtgc cgtggttgtt gataactctg ttactggcga aaggattgga
      481 agccgtatcc aagatcggca agaggagtag cggagtgttg ggttgcgctg caaccgggaa
      541 cggccggtcc atcgctgtct ggccgttgct gcagcagagg gcttattcgc ccacgccagc
      601 gcgcaggcta gcgccctggg ctcgcaattc ttgctgtact tcctcagcgg gtacatcccg
      661 cggcagcttg tcggtgcgag tagccaatgc tgcgcacacc cccgccgcgt gaccggtggc
      721 catggcgatc ggcatgacgc ggtaggacga gtgagcgacg tgtgtgccgg agatgcacct
      781 gccggcgacg atgagccgat ccactcctcg gggcagcagg cagcgcaacg ggatgtcgta
      841 ggcctggcct tttggtacgc gtttgaggac ggttccggtg ccttccggat tgtggatgtc
      901 aacgggatac gcgccgcgag cgatcgcgtc cgggaacttc ttagcggcga gaatgtcgtt
      961 cccggtcagc tggtagtccc ccaggacgcg gcgtgtctca cgtacgccca cctgcacgcc
     1021 gctctgcgca acgtaggaat tttcgaaacc tggcactcgg tcgcgcagga agcgcgagat
     1081 ctggtctagt tgccgccgag cgacgaattc ggctcggctc agatcccaca cgctcgtacc
     1141 aagcgcacga gttacccgcg tgctattcac gctgagctcg tgcgggtgcg gggtagcgaa
     1201 gaacagcagg tcctcacgcg gtagctcaag gtccccggct tcggtggcct gctggatcag
     1261 gtcccacagg ccgtgcacac cgcgccactg gtccggatgc acgcgcacgt aatcggcgaa
     1321 gcgagattgg aggaaatcga ccatccggaa catcagggtc atcggttgca caaggccgtc
     1381 ctcggtgcgt cccacttcga atgcagcacc ggaggccgca gcaacgtcac cgtcgccggt
     1441 acagtccaca acgacctgcg catcgatcac caccggtccg gatttggtct cgaacaccac
     1501 ccgcggtaat ctcgagtcgc ttgatacgcc ggaggcaaag gcgtggaata gcagtcgcac
     1561 accagcttga tcgagcatgt caagggcggc gagtttaaac tgttccggat cgaatggaac
     1621 cgtgtaaccg gtgctggctg aaggtgatat ggccccacca cgctgggtga gccgttgcag
     1681 caactgccac agcacccctg ccaccactgg gtcgccctgc ccgtggtcgg tcggcagcag
     1741 tcgggtgttc tcgcccggca ccgcctgctt tttttcgttg tgaaaggaca tcaggggcat
     1801 cacgagcgcg accgtggcgt tgcctccgag aaacccgtaa cgctcgacga gaac
//
LOCUS       DATADN010000874         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1302617, whole genome shotgun sequence.
ACCESSION   DATADN010000874 DATADN010000000
VERSION     DATADN010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302617"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /gene="eccB"
                     /locus_tag="VHH53_11340"
     CDS             complement(<1..1325)
                     /gene="eccB"
                     /locus_tag="VHH53_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HEX2290761.1"
                     /translation="MSKDPTTTSQVQAYRFVLRRMESALVRKDAVMLHEPMRQHQRAT
                     AVGLILGVLGLLAFFVVGKFSPTSQISGNEIVIGSPSEAVFVVQDNPRRLIPVLNLTS
                     ARLLLAAISPGQGASEAKRVADSALADIGRAPSTGLPGAPPLPAPENLIQGAWSVCDT
                     AKVGQDLPNHTESQPQLSTTVLVREGQPPGRWLGDDQALLIAEQTTAATYFVWRGQRR
                     QVDLRDPAVRYYRLGNVAPRKVSTGLLNAIPEGRPLTLPTVPTGRPLPELPGVQVGDV
                     VRVKGVHDSFFLVLPQGVQPVEPAAADLIRYGRSTSADFTNVTPEAISRVPEAPLASQ
                     LDLAGLPAQAPQVLKTTETPIACLTWQGPDREPVISINLDERLPLDDGERPVEVPPGA
                     SDQTVDSVFLKPTKGALVRGVVPGQRTDTGAIWLVTDQGLRYGIPTIEVA"
     gene            1463..2619
                     /gene="eccE"
                     /locus_tag="VHH53_11345"
                     /pseudo
     CDS             1463..2619
                     /gene="eccE"
                     /locus_tag="VHH53_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166816.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccE"
     gene            2626..3345
                     /locus_tag="VHH53_11350"
     CDS             2626..3345
                     /locus_tag="VHH53_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290762.1"
                     /translation="MRTLGNTTGVRAVAPEFLVTPDLLDALSPLADRGGMVLGSGPHD
                     EPLSVSVLRPQPTRLVLVGGLYLARQVALRAMATGVWVVVATGRPAAWQVLTRAAGEG
                     PDGRPAPLVQVRKLAPVELPRASEDAPLLVVHDGGAVPQELFAPRSPWQTTLYVLPYL
                     HPQVEGTANNADLVLLQRLPMDQAQLAAAMWRLSPPMTNQLAALPDDGVVALGPDLWL
                     PLRLVTTPAEREILGPVRRGD"
     gene            3620..4456
                     /locus_tag="VHH53_11355"
     CDS             3620..4456
                     /locus_tag="VHH53_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2290763.1"
                     /translation="MARVTVSEENDSPIDLYYEDHGSGQPVILIHGWPLSGRSWERQV
                     PALVEGGYRVIAYDRRGFGASSRPWDGYDYDTFAADLHKLIGHLDLTGVTLIGFSMGG
                     GEVARYVGRYGTERVAKAVFAAAVPPYLYKSADNPEGGIDDATLTGFHDGIRADRVAF
                     VDQFVTNFFAVRGRTDLVSEPLRRYNAAIAAAASPKGTLDCTTAFTKTDFRGDLREFT
                     IPTLVIHGDSDAIVPFEVSGRRTAEAVDGSKLVLVKDAPHGLNVTHAQTFNAEVLAFL
                     RS"
BASE COUNT          675 a         1575 c         1518 g          754 t
ORIGIN      
        1 gccacttcta tcgtgggtat gccgtagcgc agtccctgat cggtaaccag ccagatcgcc
       61 cccgtatcgg tgcgctggcc aggcaccact ccgcggacca gggcgccctt ggtcggcttg
      121 aggaagacgc tgtcgaccgt ctggtcactc gccccgggag gcacctcgac cggcctctcg
      181 ccatcgtcta gcggaagccg ctcgtctagg ttgatgctga tcaccggttc ccggtccggg
      241 ccttgccagg taaggcaggc gatcggcgtc tcggtggtct tgagcacctg cggggcctgg
      301 gcggggaggc cagctagatc cagctggctt gctaacggcg cctccggcac ccgtgagatg
      361 gcttccggtg tgacgttcgt gaagtccgcg ctggtgctgc ggccgtagcg aatcagatcg
      421 gctgctgcgg gttccactgg ctgcacaccc tgaggcagca caaggaaaaa ggaatcatgc
      481 actcctttca cccgcacgac gtcaccgacc tgaacacccg gaagctcggg taagggccgc
      541 ccggtcggga cggtcggcag cgtcaaaggg cgcccctcgg ggatcgcgtt gagcagaccc
      601 gtactgacct tacgcggcgc aacgttgccc aaccggtaat accggacggc ggggtcgcgc
      661 agatcaacct gtcgccgctg accgcgccac acgaagtagg tggctgcggt ggtctgctca
      721 gcgatgagaa gggcctggtc gtcgccgagc cagcgacccg gtggttgccc ttcccggacc
      781 aagacagtgg tggacagctg cggctgcgac tccgtgtggt tcggtaggtc ctgccccacc
      841 tttgcggtgt cgcatactga ccaggcaccc tggatcagat tctccggcgc cggcaggggc
      901 ggcgctcctg gaagcccggt ggacggtgcc cgcccgatgt cagctaacgc ggaatccgcg
      961 acccttttgg cctccgatgc tccctggccc ggtgagatcg ccgccaacaa caggcgcgcc
     1021 gaggtcaggt tgagcactgg gatgagccgc cgcgggttgt cctgcaccac gaagaccgcc
     1081 tcggacgggc tgccgatgac gatctcatta ccgctgatct gactggtcgg gctgaactta
     1141 cctaccacga agaatgccaa cagccccagc acgcccaaga tcaggccgac cgcggttgcc
     1201 cgctggtgct ggcgcatcgg ctcgtgcagc atcaccgcgt ccttgcggac cagtgccgac
     1261 tccatcctgc gcagcacgaa gcggtacgcc tgcacctgcg acgtggtggt cggatccttg
     1321 gacatcgggt gcctgccgct ctcccccggg gctgtacggt gcggcaggat agcgggacgg
     1381 agacgcagag atgaccggtg cactgcccgc cacggtgact gcccggcggc gctcgacagg
     1441 ctcgcgtctg ggcccgctgc ccatgatcaa cgttgtggcc ctggaggtcg gggtcgcgct
     1501 tggtctggcg ctcttcgccg tcgacaccgc cttgtggtgg gcagcgctgg cggtgctgct
     1561 gatcgcggtg ccgctggcgc tggggcggtg gcgcggtcgc tggctcgtgc tgtgggccca
     1621 actcgccgga cgctacctgt tgcgctcgcg tcgccggacc gcaacgagtc cgaacctggt
     1681 ggtggctggt cgcgcgccga ccgcaccgga gccgcccctg ggcaccgatc cccgggtcgc
     1741 cctgttgcgc ctcgtgctcc cggagctgat cgtcgcgcat accactgacc atcagcgtga
     1801 accgctgggt ctagccgggc accatggcac ctggactgct gtgctgcagg tggacgccac
     1861 accgttgatg atcgccccgg tcgggggcac tccgagcctg ccgctcggcg cactggcccc
     1921 ctgcttggag gatcgcgggg ttgtgctcga cgcgatccag gtgatttggc actgctaccc
     1981 gggaagcgcg gccctgccgt cgtcatcccc cgcactgcgc tcctacctgg aagtcttagg
     2041 cccactgccg gcggccgctc gccgcaccac ctgggtcgtg gtgcgattgg atccccgccg
     2101 atgcctcgcc gccgtcgccg aacgcggtgg tggggtggtg ggctcgcatc gggcactgat
     2161 tggcgcgctg tcccgggtgc gtagcgccct ggaatcccgg ggcatctcga cccgatcact
     2221 ggacgccgac cgcctattgc gcgctgggat ctccgccgcc gaactgtccg cggtggccgg
     2281 caccggacaa caggtggcgc tgaacgaagg atgggcggcc gtcaccgcgg ccggggtggg
     2341 acacgccagc tatgcgatca ccgactgggg caccacgccg aacctcaacg cgttgaccgg
     2401 agtccgcgca ctgtccacga ccgtcgcgct gtcgctgtcc ccggcgcgga tggcaccgag
     2461 gtcgggttgc ggggcgtgat ccgggtcagc gcacgcaatc ccaccgaact agaagcggcc
     2521 gacgaccggt tgcaggccgt ctccgaacgg ctcggtgtgt cgctgatccc gctgcgtggg
     2581 ctgcaggtcg ccggcttggc ggcgacgttg ccactgggcc tgcgcatgag gacattgggc
     2641 aatacgactg gagtccgggc ggtcgcgccg gagtttctcg tcacaccgga cctgctcgac
     2701 gcgttgagcc cgttggccga tcgcggtggc atggtcctgg gttcgggccc acacgacgag
     2761 ccgctgagcg tgtctgtgct acgcccgcag ccgacccggc tggtgctggt cggtgggctc
     2821 tacctggccc gccaggtcgc cttgcgagcg atggccaccg gcgtctgggt ggtggtcgcc
     2881 accggtcgcc ccgcagcgtg gcaggtgctc acccgagccg ccggtgaggg cccggacgga
     2941 cgtcctgctc cactggtgca ggtacgcaaa ctcgcaccgg ttgagttgcc acgagcctcg
     3001 gaggacgcgc cattgctggt ggtacatgac ggtggggcag tcccccagga actgtttgcg
     3061 ccccgttcac cgtggcagac caccttgtac gtgctgccct atctgcaccc gcaggtcgag
     3121 ggaaccgcga acaacgccga cctcgtcctg ctgcagcgac tcccgatgga ccaagcgcag
     3181 ctcgccgcgg cgatgtggcg gctctccccg ccgatgacga accaactggc ggcattaccc
     3241 gacgacggtg tggtcgcgtt gggacccgat ctgtggctac cgctgcggct ggtcaccacg
     3301 cctgcggagc gggagatcct ggggccggtg cgccgcgggg attgacaacg agtacggccc
     3361 ggctacgcac cggtgggcgc gtggccgtcg ccggctgggt gcaagcggca gccgggaact
     3421 cggccagcgc catgtccgcc gcgcggacgg cgcagcaggc cggacccacc gcggcgcggt
     3481 ccggttacca tttgggtcat tgccgccgtt gccggcgtcg tcccgatggc cagggcctac
     3541 tctgccgatg tcctgctcgc ctcggcgcac gcggtcgcca acgggcccgt catatcgaat
     3601 cagggtaagg atccacagca tggcacgcgt caccgtcagc gaagaaaacg acagccccat
     3661 cgatctctac tacgaggacc acggctcggg acagcccgtg atcctcatcc acggttggcc
     3721 gctgtccggc cgatcctggg agcgccaggt tcccgcactg gtcgagggcg gataccgggt
     3781 gattgcctac gaccggcgcg ggttcggcgc ctcgtcgcgg ccttgggacg ggtacgatta
     3841 cgacactttc gccgccgacc tgcacaaact gatcggacac ctcgacctga ccggcgtgac
     3901 tctcatcggg ttttcgatgg gcggcggcga ggttgcccgc tacgtcggcc ggtacggcac
     3961 cgagcgcgtc gccaaggccg tgttcgctgc cgccgttccc ccatacctgt acaagtccgc
     4021 cgacaaccct gagggcggta tcgacgacgc caccctcacc gggttccacg atggcatccg
     4081 agccgaccgt gtcgccttcg tcgaccagtt cgtcacaaac ttcttcgctg tccggggccg
     4141 caccgacctg gtcagtgaac cgctgcggcg ctacaacgcc gcgatcgccg cagccgcgtc
     4201 accgaaaggc accctcgact gcacgacggc attcaccaag accgacttcc gcggcgacct
     4261 tcgagagttc accatcccga ccctagtgat ccacggcgac tccgacgcga tcgtgccgtt
     4321 cgaggtcagc gggcggcgca ccgccgaagc cgtcgacggc agcaaactcg tcctcgtcaa
     4381 ggacgcaccc cacggattga acgtcaccca tgcccagacc ttcaatgccg aagtactcgc
     4441 atttctccgc agctaaccgc cagcgtcccc ggcatcctgc cctgtatcgc tcggcggcac
     4501 agccggtgat ggcagagctc gg
//
LOCUS       DATADN010000875         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_249824, whole genome shotgun sequence.
ACCESSION   DATADN010000875 DATADN010000000
VERSION     DATADN010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249824"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1556)
                     /locus_tag="VHH53_11360"
     CDS             complement(81..1556)
                     /locus_tag="VHH53_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX2290764.1"
                     /translation="MSRVAWTDPAPLEVERPRLPWWTLLPRKLLLTASPVIVVVVVVM
                     VVVFTARRVWRYPLFLACSTILAGLGLGYSWWVPMKLAAGLALVAGLWAWAHSDSFTR
                     TVVQQVRSEWRRALVYAGPWKRTMLFSDLTKHTGHRQRSTHYPTLRRVRADGWRDRVS
                     VKLLHGQCADTYAAHAAELANSFGAHSCRVRVDRPRRIWLDLLHSDPLTQPLTIPSLA
                     KPGTGVDLARVVIGRTETGRPWLLRLADRHILVAGVSDAGKSSVMWAVLRALAPWIRC
                     GLVQVFGIDPKGGMELGRAEGLFQRLVCTNGAEAIELLEHVATLTRQRAETLRRQASR
                     KWTPGSGQPFVLLILDELADVIAYQPDNGLRKRANLALQSVLSQGRAPGVCVIGQLQD
                     PRKEIIDFRHLFPVKIAMRLDEPTQVDMVLGTRVRERGAAAHEISEDTPGVAWAKIDG
                     RRDPDRARAFHHTNADLDELCAYITAGRHDAPRPLTGIEAA"
BASE COUNT          275 a          547 c          529 g          227 t
ORIGIN      
        1 ggcatgcacc gagcgcagct gcggcacgtc gaattcggtg aggtgggcgc ggatcgcgtc
       61 cagcagatca actgcgctgg tcatgctgcc tcgattccgg tcagcgggcg gggggcgtcg
      121 tggcgaccgg cggtgatgta ggcgcacagc tcgtcgaggt cggcattggt gtgatggaag
      181 gcccgggcgc ggtcggggtc gcggcggccg tcgatcttgg cccaggcgac gccaggggtg
      241 tcttcactga tctcgtgcgc ggccgcgccc cgctcgcgca cgcgggtgcc caacaccatg
      301 tccacctggg tgggctcatc cagccgcata gcgatcttga ccgggaacag gtgccggaag
      361 tcgatgatct ccttacgcgg atcctgaagt tgtccgatca cacacacccc aggtgcccga
      421 ccctgagaca agacggactg caacgccagg ttggcgcgtt tacgcagccc gttgtccggc
      481 tggtaggcga tcacatcggc caactcgtcc aggatcagca gcacgaacgg ctgccctgaa
      541 ccgggggtcc acttgcgact cgcctgccgc cgcagggtct cggcgcgttg gcgggtcagg
      601 gtcgcaacgt gttccaacag ctcgatggcc tccgcaccgt tggtgcacac cagcctctgg
      661 aacagtccct cggcgcggcc gagttccatg ccgcccttgg ggtcgattcc gaagacctgc
      721 accagcccgc accggatcca gggagccagg gcacgcagca ccgcccacat caccgacgat
      781 ttgccagcgt cacttacgcc agcgacgagg atgtgccggt cggccaagcg cagtagccat
      841 gggcggccag tctcggtgcg gccgatcact acccgtgcca gatccacgcc cgtgcccggc
      901 ttcgccagag agggaatggt gaggggctgc gtcagcgggt cggagtgcag caagtccagc
      961 cagatccgac gcggccgatc cacccgcacc cggcaggagt gggcaccgaa ggagttggcc
     1021 aactcagcgg cgtgcgcggc gtaggtgtca gcgcactggc cgtgcaacag cttgaccgac
     1081 acccgatcgc gccagccatc ggcgcggacc cggcgcagtg tggggtaatg cgtgctgcgc
     1141 tggcgatgcc cggtgtgttt ggtcaggtcc gagaacagca tggtccgttt ccacggcccg
     1201 gcgtagacca gggcgcggcg ccactctgag cgcacctgct gtaccacagt ccgggtgaac
     1261 gagtccgaat gcgcccaggc ccataggcca gccaccagcg ccagcccggc ggccagcttc
     1321 atcggcaccc accacgagta acccagtccg aggccggcca ggattgtgct gcaggccaga
     1381 aacagcgggt accgccacac ccggcgggcg gtgaacacca ccaccatgac cacaaccacc
     1441 acgacgatca ccggtgaggc ggtcagcagg agcttgcggg gaagaagcgt ccaccacggc
     1501 agccgcggcc gttccacctc cagcggcgcc gggtcggtcc acgccacccg gctcatcgga
     1561 actcaccgcc acccagcc
//
LOCUS       DATADN010000876         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1302988, whole genome shotgun sequence.
ACCESSION   DATADN010000876 DATADN010000000
VERSION     DATADN010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302988"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..>1554)
                     /locus_tag="VHH53_11365"
     CDS             complement(415..>1554)
                     /locus_tag="VHH53_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX2290765.1"
                     /translation="VPGRLSPVPCSLSPEMKPFTIDPNIARAQTLPAEVYASPAWYAL
                     QKERVFARSWQMVRGAERVKAPGHLLPFTLLEGTLDEPLVLSAGEDGVLRCLSNVCTH
                     RGTVVCEGETHAKHLRCRYHGRRFALDGRMTHMPEFDGVENFPSPSDDLPRLPLHSWG
                     PLTFTAIDPACPFDEWIGPVRDRVGFLPLDEFRFDPATSRDYVIDANWALYCDNYLEE
                     FHLPYIHASLSEALDYASYRTETYDWCNLQLGTTKDRREAFDLPAGHPDSGELIAAYY
                     WWLFPNLMLNFYPWGLSVNLVQPVAQDRTRVSFLSYVWDASRRSSGAGADLHRVEMED
                     EEVVEAVQKGVRSRLYHRGRYSPRREVGTHHFHTLLARETGSVAG"
BASE COUNT          239 a          553 c          531 g          231 t
ORIGIN      
        1 caccctacca cccaggggga cctcatgcgt ggcaagcttc tgctgaacct ggacgacctt
       61 tcggtggaca gcttcgacac caccgccacc cagacccgca ggggcacggt cctgggcgag
      121 gagtgcacct gccccagcgc ctgcagctgt ccgggctgcc ccacctgtcc agcgacgtgc
      181 gggctcacct gcgacgaccc ttcatgcggc gaaacttgcg atagctgcat gtactcggag
      241 tgcgatacgt gcccgtacac gtgcggcacg aactcgtact gcgccgattg ccagggctga
      301 gggccgcgcg ggcgggggcg ccacaaccgg cgctcccgcc tgcttgattc cgtccaccgg
      361 gtcccggcag tactgctgcg gcaggtctcc gtttgggacc gccgccgcgc tctctcaccc
      421 cgccacgctc cccgtctcgc gcgccagcag ggtgtggaag tggtgcgtgc ccacctcgcg
      481 gcgcggggag tagcggccgc ggtggtacag gcgcgagcgc acgcccttct gcaccgcctc
      541 caccacttcc tcgtcctcca tctccacgcg gtgcaggtcc gcgccggcgc cgctggaccg
      601 tctgctcgcg tcccacacgt aggagaggaa cgagacgcgg gtgcggtcct gcgccaccgg
      661 ctgcaccagg ttcaccgaca gcccccaggg atagaagttc agcatcaggt tggggaacag
      721 ccaccagtag tacgccgcga tcagctcccc ggaatcgggg tgcccggcgg gaaggtcgaa
      781 cgcctcgcgg cgatccttcg tcgtcccgag ctgcaggttg caccagtcgt aggtctcggt
      841 gcggtagctg gcgtagtcca gcgcctcgga gagcgaggcg tggatgtacg gcaggtggaa
      901 ctcctccagg tagttgtcgc agtacagcgc ccagttggcg tcgatcacgt agtcgcgcga
      961 cgtagcgggg tcgaagcgga actcgtccag cggcagaaag cccacgcggt cgcgcaccgg
     1021 tccgatccac tcgtcgaacg ggcaggcggg gtcgatggcc gtgaaggtga gcggccccca
     1081 gctgtgcagc ggcagccgcg gcaggtcgtc cgacggggaa gggaagttct ccaccccgtc
     1141 gaactcgggc atgtgcgtca tcctgccgtc cagcgcgaag cgccggccgt ggtagcggca
     1201 gcgcaggtgc ttggcgtgcg tctcgccctc gcacaccacc gtgccgcggt gggtgcagac
     1261 gttggatagg caccgcagca ccccgtcctc tcccgcggag agcaccagcg gctcgtccag
     1321 cgtcccctcc agcagggtga acggcagcag gtgccccggc gccttcaccc gctcggcgcc
     1381 gcgcaccatc tgccagctgc gcgcgaacac ccgctccttc tgcagcgcgt accacgcggg
     1441 cgaggcgtac acctccgccg gcagcgtctg cgcgcgggcg atgttcgggt cgatggtgaa
     1501 cggcttcatc tcaggggaca gggaacaggg gacaggggac agccggccgg gcac
//
LOCUS       DATADN010000877         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1303100, whole genome shotgun sequence.
ACCESSION   DATADN010000877 DATADN010000000
VERSION     DATADN010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303100"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..571)
                     /locus_tag="VHH53_11370"
     CDS             complement(287..571)
                     /locus_tag="VHH53_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290766.1"
                     /translation="MANLSQSLLPFADAPVDAPPLAGAAAPESGPEPEPAQTTPHFPG
                     VARMVDLAPQPIVSFAGVLAGAAVPAAAPAALDFPGVAGMADLANPLVAA"
     gene            584..769
                     /locus_tag="VHH53_11375"
     CDS             584..769
                     /locus_tag="VHH53_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290767.1"
                     /translation="MKTWSGLSRPRPRFRRSRAHRRSRAHRRSGADRRIDKGFGRADH
                     YVNAKETSNDLGHEPQG"
     gene            1668..2336
                     /locus_tag="VHH53_11380"
     CDS             1668..2336
                     /locus_tag="VHH53_11380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2290768.1"
                     /translation="MTRISAVLFDVDGTLVDTNYLHAVTWWEAFRQYGHHVPMAHIHC
                     TIGMGSDKLLDHLLPGDRDRAADDEMRSAHTALYAPYWPRLRAFDGAADLLRACASRD
                     MRVVLASSANEREMRALRDALNAEDVVTTATSAGDAEQSKPAPDILQIALERGEVAPD
                     AAVFVGDTVWDVQACKKVGLDCIAFLTGGTSREELYEAGAAEVYQGPAELLESLQDSL
                     IAQR"
     gene            complement(2389..2625)
                     /locus_tag="VHH53_11385"
     CDS             complement(2389..2625)
                     /locus_tag="VHH53_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290769.1"
                     /translation="MGTATGEAGQVTGTKDKDYNLIWFVEQCLSNALRLETYIQDAER
                     DGDSELADFFRRAQGESRKGGEQGKAQLRKRLAG"
BASE COUNT          498 a          921 c          970 g          457 t
ORIGIN      
        1 ccgtgcgaac gttcgccgct gcgtcagggc aagagatcat cacgccgaaa ctgatcgtct
       61 acgatgccca agtccagcgg gctttcccgg cggctgtgcg gtgttcgcct gagtgcgcga
      121 cgtgcggtac tcagggcgtt ataggagtag ctgatctggc gccgttgcag gctgcgccga
      181 cggctggacc cgctttcgcg ccagcttcac tcgaactagc tggagtcagg catgccggat
      241 ccgattccac ggaatagaag tgcgggtaga tctcggccgc gccggtttac gcggcgacca
      301 agggattggc caggtcggcc attccggcaa cgccgggaaa atccagagca gccggcgccg
      361 ccgccggcac agccgcgccc gccagcacac cagcaaagga cacaatgggc tggggggcta
      421 ggtcaaccat tcgggcaacg ccggggaaat gcggagtggt ctgagccggc tccggttccg
      481 gccccgattc cggcgcagcc gcgcccgcca gcggaggtgc gtcgaccggc gcatcggcaa
      541 agggcagaag ggattggctt aggttggcca ttcgggcaac gccatgaaaa cgtggagcgg
      601 tctgagccgc ccccgccccc ggtttcggcg cagccgcgcc caccggcgca gccgcgccca
      661 ccggcgcagc ggcgccgacc ggcgcatcga caaagggttc ggccgcgcgg accattacgt
      721 caacgccaag gaaacgtcca acgacctggg ccacgagccc caaggctgag gctgacgcgg
      781 tccaacagtc aggtcgtggt gggtgtatta ctttgtgcag tggcggcgct tgcagtaggg
      841 atcgtcgtgc cgccgctgtt gtcggcggat acgccttccg gctcggtggt ggctgcgtca
      901 acagatggtc aggccggcgt tgtggacgag ggcagtggtg acccgggtca ggtcggtggc
      961 gagtttgctg cgcctcctgc agcgccaggc gccgattccg tccaaacgca gcccgcccca
     1021 gcgctggttg gtaatcggac cgcggtgccc cctggtggtg gatcgtcagt gccggcaggc
     1081 cagcccggtc tagcagcaga gccggccccg ccagctgatc tcgggcagcg agttgaccgg
     1141 ccggcagacc agacccagcg atctgctgat cggcaaggta accctgagtc tggcaatccc
     1201 cgcccgcgga cgaacccccc gccgccaccg cctccagcaa cgcctccacc cagtgacggc
     1261 gcgacgcgag cagagtatga gaaggccatc caagcagcca ttcagaagta ccagtcaaaa
     1321 tcccagggat cgaacccgcg cggcggcggt aacgataacg gtggtgcgac gggaggccgg
     1381 gacacatcag ggaagccttc agggaggacg gactcgggcg gcgacggcgg acccggcgga
     1441 tcgacgaaag gtggggacac gtcaggagcc ggatcctcaa tgaaaacggg tggcggcaag
     1501 cgaagcggcg gatcggggag caccggatca ggaggctgat gcgatcacgt gatcgcgcga
     1561 cggcgccgcg tgaggagggg tgaccgggac gcggcggtca cccctgctgg aacacccacg
     1621 cgcgatccgg cgcataccac cgcgatgccg tgggtagttc ttgcagcatg actcgaatct
     1681 ccgcagtcct gttcgatgtg gacggtaccc tcgtcgatac caactacctg catgccgtta
     1741 cctggtggga ggcgtttcgc cagtacggcc accacgtgcc gatggctcac attcactgca
     1801 ccatcggtat gggctcggac aagctgctcg atcatctgct gccgggcgat cgcgaccgtg
     1861 cggccgacga cgagatgcga tccgcacaca ccgcgctgta tgccccgtac tggccccggt
     1921 tgagagcctt cgacggcgcc gcggacctgc tgcgggcgtg cgccagccgg gacatgcggg
     1981 tggtgctggc aagctcggcc aacgaacgtg aaatgcgtgc gctgcgcgac gcgctcaatg
     2041 ctgaggacgt tgtcaccacg gcgacctccg ctggcgacgc ggagcagagc aagccggcgc
     2101 cggacatcct gcagatagcg ctggagcgcg gcgaggtcgc ccccgacgcg gcggtcttcg
     2161 tcggagatac cgtctgggac gtgcaggcgt gcaagaaggt cgggctcgac tgcatcgcat
     2221 ttctcaccgg cggtaccagc cgcgaggagc tgtacgaggc gggtgcggcg gaggtgtacc
     2281 agggtcccgc cgagctactc gaatcccttc aggacagcct catagcccaa cggtaggcct
     2341 gtggtgggag cccaaaggga gccgattgat cgtttctgac cctggtgttc agccggccaa
     2401 gcgcttgcgc aactgcgctt tgccctgctc gccccccttg cggctctcgc cctgggcgcg
     2461 gcggaagaaa tcggccagct ctgagtcacc atcgcgttcg gcgtcctgga tataggtctc
     2521 caggcgcagg gcgttgctca ggcactgctc gacgaaccag ataaggttgt agtccttgtc
     2581 cttcgtcccg gtgacctgcc cggcctctcc agttgcggtg cccatccgtg gctcctctct
     2641 cgcggatctg cttgcactgt tgctctaccc cttgcagcgc cggcctacac ccagtgcggg
     2701 cactcgatcc ccgcaggctc acggacagtg acatcgtgta ccttgcgtta acaaactgag
     2761 cggttgccaa atgcgcttgc cgaaagggga ggggacacat gaagcggctt ttggccatgg
     2821 ccatcctgct cgtgttgtca gcggcg
//
LOCUS       DATADN010000878         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_93695, whole genome shotgun sequence.
ACCESSION   DATADN010000878 DATADN010000000
VERSION     DATADN010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93695"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VHH53_11390"
     CDS             complement(<1..424)
                     /locus_tag="VHH53_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX2290770.1"
                     /translation="MATAPVGAELAASLPDRVRVHALAKLLSVSSKEVMAALTELGIA
                     IRSAQSSIDRETALRALQVLLPDPASDAVETEQFDLQAEEVDDRGTPGVSLAPVFNSA
                     APVFLPPVSKSAVVEEPDDEEDEDREGEAEPDGASRRRG"
     gene            complement(610..1422)
                     /locus_tag="VHH53_11395"
     CDS             complement(610..1422)
                     /locus_tag="VHH53_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX2290771.1"
                     /translation="MRHNPSAAPVCKVRLRYAKRGKLRFTSHRDVARAFERALRRAGL
                     PMAYSQGYSPHPKISWVGAAPTGAASEAEYVEIQLVDHVDPEMVRGELDAALPEGLAI
                     LEAVLAGPGGLAERIEASRWHIEVRQWGIEQLRHATQALLAVPELQVQRVTKDGLRSL
                     DARAAIVSLEVEEFPTLAPAPDQLIRCGILLAVVRQVNPAVRSDDVLSALGVVAGLAP
                     PVPAKAIRMAQGRLDDYGDLVDPLAADRAYARAVVVGNDGGPDQHDVAGHAG"
     gene            1549..>1801
                     /locus_tag="VHH53_11400"
     CDS             1549..>1801
                     /locus_tag="VHH53_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX2290772.1"
                     /translation="MLLRLLRIVAVVGAVLVVLKSPSPDVAYAAPAPPGPPPDCASTG
                     PLLRYVTLFDAETPERRAAREIRAACGNSTAYYPQIGVAV"
BASE COUNT          268 a          661 c          579 g          293 t
ORIGIN      
        1 ggccccggcg ccggctggcc ccgtcgggct ccgcctcgcc ctcccggtcc tcatcctcct
       61 cgtcgtcggg ttcctcgacg accgccgact tcgacaccgg cggcaggaag accggtgctg
      121 ccgagttgaa taccggcgcc agtgacacgc caggtgtacc gcggtcatcg acctcctcgg
      181 cttgcaggtc gaactgctcg gtctccaccg cgtcggatgc gggatccggc agcagcacct
      241 gcagtgcccg caacgcggtt tcccggtcga tgctggactg cgcgctgcgg atcgcgatgc
      301 cgagctcggt cagcgcagcc atgacttcct tgctgctcac cgagagcagc ttggccaggg
      361 cgtgcacgcg caccctgtcc ggtagggaag cagcaagctc agccccgacg ggggcggtgg
      421 ccatggatgt gcccggtagc ccgccgatgc ttccggcagg cgttgtcctg ttcgccattc
      481 aactcctcca gcccccgggc gcgtcccgtg tgtcggaacg cggccgcgca ggggccctat
      541 gcgttatccg ccgccaagcc tggcgacggc aatcctgtgt tccaccggca gctccgcggt
      601 tcggaacggt caaccggcat gtcccgcgac atcgtgctga tccggcccgc cgtcgttgcc
      661 caccaccacc gcccgggcgt atgcccgatc cgcagcgagc gggtcgacaa gatccccgta
      721 gtcgtcgagc cgaccttgcg ccatccggat cgctttcgcg ggcaccggcg gcgcaagacc
      781 ggcgacgacg ccgagcgcac tcagcacatc gtcggatcga accgcagggt taacctgccg
      841 tacgaccgct agaagtatcc cacaccggat gagttgatcg ggcgcaggcg ccagcgtggg
      901 gaattcctcg acttccaacg agacgatcgc cgcccgcgcg tccaacgagc gcagcccgtc
      961 cttggtgacc cgttgaacct gcagttctgg tacggcgagc agcgcctgag tagcgtggcg
     1021 tagctgctcg atgccccact ggcggacctc gatgtgccac ctgctcgcct cgattcgctc
     1081 ggcgagtccg ccaggaccag ccaggaccgc ctccaggatg gcaagcccct ccggcaaagc
     1141 cgcgtccagc tcgccgcgga ccatctccgg atcgacgtga tcgacgagct ggatttcgac
     1201 gtactcagcc tcgctggccg cgccggtggg cgccgcgccg acccaggaga tcttcggatg
     1261 cgggctgtag ccctgcgagt aggccatcgg aaggccggcc cgccgcagcg cacgctcgaa
     1321 cgctctggcg acatcgcggt gtgaggtgaa tcgcagcttt ccacgcttgg cgtagcgcag
     1381 ccgcaccttg cagaccgggg cggcagaggg attgtggcgc atcgaaacct ctcgtgagtg
     1441 cgtaacggtg ttctagcacg ccatgccact cacggccccg cttgtcaccc ctggcgcagc
     1501 tcactacggt cacgacgtca gtccacctgc caaatctgga ggctgtgggt gttgcttcgt
     1561 ctgctgcgga tcgtcgcggt cgtcggagcg gtgctcgtcg tgcttaaatc gccgtccccc
     1621 gacgttgcct acgccgcgcc cgcgccgccg ggcccaccgc cggactgcgc ctcgaccggt
     1681 ccgctgctgc gctacgtcac gctgttcgac gccgagacac ccgagcggcg agccgcgcgc
     1741 gagatccggg ccgcctgcgg aaacagcacc gcctactacc cgcagatcgg ggtcgccgtc
     1801 g
//
LOCUS       DATADN010000879         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1172401, whole genome shotgun sequence.
ACCESSION   DATADN010000879 DATADN010000000
VERSION     DATADN010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172401"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..209
                     /locus_tag="VHH53_11405"
     CDS             21..209
                     /locus_tag="VHH53_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2290773.1"
                     /translation="MPIRRLGRPEDVAAAVGFLSSPQATWVTGVNLPVGGGHHLRRAP
                     RFDGYVRRQHGQDWLPPR"
     gene            complement(226..1212)
                     /locus_tag="VHH53_11410"
     CDS             complement(226..1212)
                     /locus_tag="VHH53_11410"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX2290774.1"
                     /translation="MNTPLLQVSGLRKHFPVRRGLLQRKVGAVQAVDGLDFEVLRGET
                     LSLVGESGCGKTTTGRLLTRLLEPTAGSIVFEGRDITHLPAGRMRPLRRDIQMIFQDP
                     YSSLNPRHTVGTIVGAPFRIQDVDPPGGVRAAVQQLLELVGLNPEHYNRYPHEFSGGQ
                     RQRIGIARTLALRPKLIVADEPVSALDVSIQAQVLNLLEDLQDELDLTYLLIAHDLSV
                     VRHVSDRVAVMYLGKIVELGDRDAVYSRPMHPYTVALLSAVPVLDPKQRSGNRRILLT
                     GDVPSPLNPPPACRFHTRCWKAQEICRTQEPPLAELAPSHRVACHFPENAPA"
     gene            complement(1209..>1539)
                     /locus_tag="VHH53_11415"
     CDS             complement(1209..>1539)
                     /locus_tag="VHH53_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2290775.1"
                     /translation="RAVEYGPASQVFSSPQHPYTWGLLGSVPRWDRERTERLASIPGN
                     PPSLISVPSGCPFHPRCRYGELTGGQASSVRPELRPALSADHLVACHLPDQRRQHILD
                     DEIRPNL"
BASE COUNT          242 a          521 c          542 g          234 t
ORIGIN      
        1 gctatgagta cctcgacaac atgccgatcc ggcgattggg acgacccgaa gatgtcgctg
       61 ctgccgttgg cttcctctct agtccgcagg cgacctgggt cacgggcgtc aacctgcccg
      121 tcggcggcgg ccaccacctt cgccgcgcgc cccgattcga cggatacgtt cgccgccaac
      181 atggccagga ctggttgccg cctaggtgac tgcgctaaca cagcgtcagg ccggcgcgtt
      241 ctcggggaag tggcaggcga cgcggtggct gggcgccagc tcagccagcg gcggctcttg
      301 ggtgcggcag atctcttggg ctttccagca gcgggtatgg aaacggcaag ccgggggcgg
      361 gttcaagggg ctgggcacgt cgccggtgag caggattctg cgattgccgc tgcgctgttt
      421 ggggtcgagt accggcaccg ccgaaagcag cgccaccgtg tagggatgca tcggccggga
      481 gtacacggcg tcgcggtcac cgagctcgac gatcttgccg aggtacatga cggcgacccg
      541 gtccgacacg tgccgtacca ctgacaggtc atgcgcgatc agcaggtagg tcaggtccag
      601 ctcatcctgc aggtcctcca gcaggttgag cacctgggcc tggatcgaca cgtccagcgc
      661 cgagacgggc tcgtcggcca cgatcagctt cggccgcagc gccagcgtgc gcgcgatgcc
      721 gatgcgctgg cgctggccgc cggagaactc gtgcgggtac cggttgtagt gctcgggatt
      781 cagaccgacg agctccagca gctgctgcac cgccgcacgc accccaccgg gtgggtccac
      841 gtcctggatc cggaacgggg cacccacgat ggtgccgacc gtgtgccggg ggttcagcga
      901 cgagtacggg tcctggaaga tcatctggat gtcccggcgc agcgggcgca tccggccagc
      961 cggtaggtgc gtgatgtcgc gcccctcgaa gacgatcgat ccggcggtcg gctcgagcag
     1021 ccgggtgagc agccgcccgg tggtcgtctt gccgcagccc gactcgccca ccagcgacag
     1081 cgtctcgccg cgcagcacct cgaagtccag cccatcgacg gcctgcacgg cgccgacctt
     1141 gcgctgcagc aggcctcgcc ggaccgggaa gtgctttctc aatccggaga cctgcaacag
     1201 cggcgtgttc acaggttcgg ccggatctcg tcgtcaagga tgtgctgccg gcgttggtcg
     1261 ggcaggtggc aagcgaccag gtgatccgcg gacagcgccg ggcgcagctc gggccgcacc
     1321 gagctcgcct ggcccccggt cagctcgccg taccggcagc gcgggtggaa cgggcaacca
     1381 gacgggacac tgatcaggct cggcggattg cccgggatcg acgccaaccg ctccgtccgc
     1441 tcccggtccc agcgtggcac cgagccgagc agtccccacg tgtaggggtg ctggggcgag
     1501 gaaaacacct gactcgccgg cccgtactcg acggcccgc
//
LOCUS       DATADN010000880         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_712245, whole genome shotgun sequence.
ACCESSION   DATADN010000880 DATADN010000000
VERSION     DATADN010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712245"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="VHH53_11420"
     CDS             <1..1665
                     /locus_tag="VHH53_11420"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX2290776.1"
                     /translation="GATLTPLAEVGAAMGLGPHLLEPYGEHVMKVKPAAIEELAGAPR
                     AKYVVVTAVTPTPLGEGKTTTTVGLGQGFAHIGKRATVAIRQPSMGPTFGIKGGAAGG
                     GYSQVVPMETLNLHLTGDMHAVTAAHNLLAALIDNQLHQHDGADLDPHSITWNRVLDV
                     NDRALRNIVVGLGSRADGNPRQSGFDITAASEVMAVLALSTSLQDMRSRLGRIVVGQT
                     YDGKPVTADDLHAAGAMTVIMREAIKPNLLQTLEGTPVLVHAGPFGNIAHGNSSVIAD
                     LIGIHCGDYLVTEAGFGADMGAERFFNIKCRASGLRPDAAVLVATVRALKTHSGRYKV
                     GAGRPLPDGMLAENPDDVHAGAANLRKQIENVRIHGVSPVVAINAFPTDHPSEHAAIR
                     EIADNMGVRSALCTHFAEGGKGAAELATAVAEVAEEPSEFAFLYSNDATLREKILTVA
                     LRVYGAQGVEYDLKASRQLESYERNGFGKLPVCIAKTHLSISSDPALKGAPSGWVLPV
                     REARASVGAGFIYPICGDMRTMPGLGATPAATRIDIDSRGEIVGLS"
     gene            1662..2258
                     /locus_tag="VHH53_11425"
     CDS             1662..2258
                     /locus_tag="VHH53_11425"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX2290777.1"
                     /translation="MIPARDIPLLDCSVNDLLVRFAAKQPAPGGGVAAAFTAAMAAGL
                     LGMAARFSTKQLIDSAGRAAQADRLRNQVAALAEQDVEAYQDVLDAFALPRELDPEGR
                     REQIRRTLERAAQVPAEIAEAASAIAIESVQLAERGNGNLRGDAFTAATLAAAAARSA
                     AELVRLNVELGDLDSDLADQAARVADVAAEVVAVLSRS"
     gene            2255..>2593
                     /locus_tag="VHH53_11430"
     CDS             2255..>2593
                     /locus_tag="VHH53_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290778.1"
                     /translation="MTGWHRMTVDGFALFDTVLGRCGIAWGPRGIISVQLPEASVCAT
                     RARVLQQCPDADEISPPPTVQQAIDGIVALLHGEAGDFSKVGDLRAIELDMDRVPPFH
                     RRVYHVARTIA"
BASE COUNT          428 a          856 c          887 g          422 t
ORIGIN      
        1 ggtgccaccc tcaccccgct ggcggaggtt ggcgccgcga tgggattggg tccacacctg
       61 ctcgagccat acggtgagca cgtcatgaag gtcaagccag cggccatcga agagctggct
      121 ggagctcccc gagccaaata cgtggtcgtc acggctgtca cgccgacccc gctcggcgaa
      181 ggtaagacga ccaccacggt gggcctgggc cagggcttcg cgcacatcgg caagcgggcc
      241 acggtggcaa tcaggcaacc ctcgatgggc cccaccttcg ggatcaaggg tggggcggct
      301 ggtggcggct acagccaggt cgtgccgatg gagaccctga acctgcacct gaccggagac
      361 atgcatgccg tgaccgcggc gcacaacctg ctcgccgcgt tgatcgacaa tcagctgcac
      421 cagcacgacg gcgccgatct ggacccgcac agcatcacct ggaaccgggt gctcgacgtc
      481 aacgacaggg ccttgcgcaa catcgtggtc ggactcggtt cacgggctga cggcaatccc
      541 cggcagagcg ggttcgatat caccgcagcc agcgaggtga tggcagtgct cgcgctgtcc
      601 acgtcgctgc aggacatgcg atcccggctg ggtcgaatcg tggtgggcca gacgtatgac
      661 ggcaagccgg tcactgccga cgacctgcac gctgccggcg cgatgaccgt catcatgcgc
      721 gaggcgatca agccgaacct gttgcagacg ctggagggca caccggtgct ggtgcacgcc
      781 ggaccgttcg gcaacatcgc gcacggcaac tcgtcggtga tcgccgacct gatcggtatc
      841 cattgcgggg actacctcgt caccgaggcc ggcttcgggg ccgacatggg cgccgagcgc
      901 ttcttcaaca tcaagtgccg cgcgtcgggg ctgcggccgg acgcggctgt gctggtggcc
      961 acggtgcgcg cactcaagac ccactccggg cggtacaagg ttggggcagg ccgaccgttg
     1021 cccgacggca tgctggcgga gaacccggat gacgtgcacg ccggtgcggc caaccttcgc
     1081 aagcagatcg agaacgtgcg gatccacggg gtgtcaccgg tggtggcgat caacgccttc
     1141 cccaccgatc acccatcgga gcacgccgcc attcgcgaga tcgccgacaa catgggtgtt
     1201 cgttccgcgc tgtgcacgca cttcgccgag ggcggcaagg gagccgccga gctggccaca
     1261 gcggtcgccg aggtcgccga agagccgtcg gagttcgcct tcctgtattc caacgacgcg
     1321 acgttgcggg agaagatcct caccgtagcg ctgcgggtat acggggcgca gggggttgag
     1381 tatgacctca aggcgtcccg gcagctggaa agctatgagc gcaacggatt cggaaagctg
     1441 ccggtgtgca tcgccaagac acacctgtcg atctcgtcgg atccggcgct caagggcgcg
     1501 ccctccggat gggtcctgcc ggtgcgggag gcgcgggcct cggtgggtgc cgggttcatc
     1561 tatccgatct gcggagacat gcgcaccatg ccgggtctgg gcgccacccc cgccgccact
     1621 cggatcgaca tcgattctcg cggcgagatc gtcggcctct catgattcct gctcgagaca
     1681 ttccgctgct ggactgctcg gtcaacgacc tgctggtccg cttcgccgcg aagcagcccg
     1741 cgccgggcgg tggcgtggcg gcggcgttca ctgctgcgat ggctgcggga ctgctcggga
     1801 tggccgcgcg gttctccacc aagcaactga tcgactccgc ggggcgggcg gcgcaggccg
     1861 accggcttcg gaaccaggtc gctgccctcg ccgagcagga tgttgaggct taccaggacg
     1921 tgctcgacgc gttcgcgtta ccccgtgaac tcgacccgga gggtcgtcgc gagcagattc
     1981 ggcgcacctt ggagcgtgcc gctcaggtgc cggccgagat tgccgaggcg gccagtgcta
     2041 tcgccatcga gtcggttcag cttgctgagc ggggcaacgg caacctgcgt ggcgatgcct
     2101 tcacagcggc caccctggca gcggccgcgg cacggtcggc cgctgaactg gtccggctca
     2161 acgtcgagct cggtgatctc gattctgacc tggccgatca ggccgcccga gtcgctgatg
     2221 tcgccgccga ggtggtggcg gtgctcagcc gttcgtgaca ggctggcatc gaatgacagt
     2281 agatggtttc gcgctcttcg ataccgtgct gggccgatgc ggcatcgcgt ggggccctcg
     2341 cggcatcatc agcgtgcagc tgccggaagc cagtgtgtgc gccacccgcg cacgagtact
     2401 ccagcaatgt cccgacgcgg acgagatatc gccaccgccc accgtgcaac aggcgatcga
     2461 cgggatcgtt gcgctcctgc acggcgaggc tggtgatttc tcaaaggtgg gcgatctccg
     2521 cgccatcgag ctggacatgg accgcgttcc gccgtttcac cgccgcgtct accatgtcgc
     2581 ccgcaccatt gcg
//
LOCUS       DATADN010000881         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_565674, whole genome shotgun sequence.
ACCESSION   DATADN010000881 DATADN010000000
VERSION     DATADN010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565674"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..740)
                     /locus_tag="VHH53_11435"
                     /pseudo
     CDS             complement(375..740)
                     /locus_tag="VHH53_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            1438..1953
                     /locus_tag="VHH53_11440"
     CDS             1438..1953
                     /locus_tag="VHH53_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290779.1"
                     /translation="MGLGGAGSMWRHVIVCCAQAFNGPGLSAHFKLFALVSTARDAGS
                     GRVEADMLADHLRYWLRVLGEFVPTRRPRLVFTVFNAPVLTERFTDVIHPALAAESSG
                     VELVADQTRTQGAGYYKQAAIGLRARDGDREVDLGDGGITTWTAALLGDAKERCVVSC
                     VATERLAALSC"
     gene            2138..2533
                     /locus_tag="VHH53_11445"
     CDS             2138..2533
                     /locus_tag="VHH53_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2290780.1"
                     /translation="MHAETDLVALLRRLSPQLREGVYVYTIVFEAIPPGAVPVVVVRE
                     DEGLTLVLPQQQADDLGLSYNFVAAWITLQVHSALSAVGLTAAVSRVLTEAGISANVV
                     AGFTHDHLFVPHDRADDAVRTLVALAATS"
BASE COUNT          436 a          887 c          896 g          499 t
ORIGIN      
        1 ccacggccag tgatcatgcc agacggccac tgcccgtggc gttacgactg ctttatctga
       61 tcttcggtag ctcgtggcct ggcttggact gctggtccgt agctcgagag cgaagaatgc
      121 ggagatcctc ttgctccggc atgaagtctc cgtgctccgc cgccaggtga gcagaccacg
      181 tctgtcctgg gccgaccggg cggtgttcgc ggcgctggcc cggttgttgt cccaggctgg
      241 cccactacat cggatcgtca cccccgagag tgtgctgtgc tggcaccgcg acctggtagc
      301 gcgacgctgg acgcagcccc atcgccgccg cacaccgagc cgctcgacag caccggactc
      361 cgtcggctga tcgcgccacc tgactcgcgc ttccagtccc agaccctgtc cacgaaccgc
      421 tcgcgcccca agtcgtgccg gcattctccc tcggccgcga gctgacgctc cacgacgttt
      481 tgggtggcga tgccggcgtg gtccatcccg ggcagccaca acacgtcata agcctgcatc
      541 cggcgccgcc gggtcaagat gtcgatcagc gcgtggtcca tcgcgtggcc caagtgcagg
      601 ctgccggtga cattcggcgg cggaatcacg acgcaaaacg ccggcttacc gctggccgga
      661 ccgccagcga aatagccagc agcaacccac cgctcataaa gccgctgctc cacctcaccc
      721 ggcacccacc tagccggcaa agacgcaccc tgctcgggac atatcttggc caccacccga
      781 gcctacgagt cccgactcga cagcctcgat cgtgtgaaac cccatgatct cgcccgatcg
      841 accacaggaa cgcgctgaca cccgcgcgaa tggctcttgc ctttcgggta tttggtcatt
      901 ttctgacgca gcggcgctgt agtgccgcaa gcgcagcttg acggcgccct gacctgtgag
      961 tcacaaggga tcagtaaccg gaacaggctc ggggcgagaa acggtgaggt ttccgatcat
     1021 cgtctgggca aggatctgcg gtcatggacc ggatatggcg agtggtgcag gcttcacctc
     1081 gcgcggcgct agagcaggag ttgtcaccga cggatcggca gtcgttgttg ctggcggtgg
     1141 cgcgcacgcg cgctagtcgc atcaccgcgg cccgcgtggc tcggcgctgg cgaaaggacc
     1201 gcttcgtgcg acccgccgca caggatcctc gcaggttggt ccaagtcgaa gccgggttgt
     1261 gggcgatgct cccggatgcc ttcgccggga tcgagctgtc cccggtggtg ccgttgggca
     1321 tgtgttcagc ggtggccccc gtggatcaga atcgggtcgt cagcacggtg cggggcaccg
     1381 aggtgctcag cgacccgacc aacgctctgg ctgtggaggc agcgtcacga cgtgcgggtg
     1441 ggcctcggcg gagccgggtc gatgtggcgg catgtcatcg tgtgctgcgc gcaggccttc
     1501 aacggacccg gtctatcggc tcatttcaag ctcttcgcgc tagtgtccac cgcccgcgac
     1561 gcagggtcgg ggagggtgga ggcggacatg cttgccgatc acttgaggta ttggttgcgg
     1621 gtgctcggcg agttcgtgcc aactcggcgg ccccgtctgg tgttcaccgt cttcaatgcc
     1681 cccgtgctca ccgaacggtt caccgacgtc atccacccag ccctggccgc ggagtcctct
     1741 ggggtggagc tagtagccga ccagacgcgc acccagggcg ccggctacta caagcaggcc
     1801 gcgatcggat tgcgtgcccg cgacggggac cgcgaggttg atctgggcga tggcgggatc
     1861 acgacctgga ccgcggcgct gctcggtgac gccaaggagc gctgtgtcgt ctcctgtgtg
     1921 gccaccgagc gtctcgctgc cctgtcttgc tgaccctcgt gagtccgatc gatgaccagc
     1981 ctgcgcccaa atcggcgata agccgtcgcc ggctgcaagt cagctgacca accggcctga
     2041 ggccacgtcc cacctgcacc gtgggacgga ggcgctgggt tgcgccgtcg ggctcgatgg
     2101 atgtgactag tgcagcgacg agcatggagg tgtagcggtg catgcagaga ctgacttggt
     2161 ggccttactg cgccgtttgt ctccgcagtt gcgtgaaggc gtgtacgtct acacgatcgt
     2221 tttcgaggcc atcccgccgg gcgcggtccc tgtcgttgtc gtacgcgagg acgaaggatt
     2281 gacgctggtg ttgcctcagc agcaagccga tgaccttggt ctgtcctaca acttcgtcgc
     2341 cgcctggatc accctgcagg ttcattcggc cctcagcgcc gtcggcttga cggcagcggt
     2401 cagccgagtc ctgactgagg cggggatcag cgccaacgtg gtcgccggat tcacccatga
     2461 ccacctgttt gtgccccatg accgggccga tgacgccgtc cgtaccttgg tggccctcgc
     2521 tgccaccagc tgagcatccg acagttcgat tgggcttccc ctcgtagcgt ggagacatcg
     2581 ccttagagga ggcgggggcg atggcggagt cacgacggaa atttgatcag gatttcaagg
     2641 atggcgcggt ccggctcgtg cgcgagaccg ggcgtccgat cgcgcagatc gcgaaggatc
     2701 tggggatcaa tcgggcac
//
LOCUS       DATADN010000882         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1022171, whole genome shotgun sequence.
ACCESSION   DATADN010000882 DATADN010000000
VERSION     DATADN010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022171"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VHH53_11450"
     CDS             complement(<1..310)
                     /locus_tag="VHH53_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2290781.1"
                     /translation="MDAGVDTIEHCTWLSGPGGTFQPDEKIARDIVAAGIAVCTANAN
                     NWRPMAARFGAEGAQQIVGRVRWLADQGVRLITGTDAGMGPFGNFPVALQGFALWGFT
                     A"
     gene            470..1219
                     /locus_tag="VHH53_11455"
     CDS             470..1219
                     /locus_tag="VHH53_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2290782.1"
                     /translation="MAEQLTISELAARTGISVRTIRFYTGMGLILPPMVRGRLGLYDE
                     RHVARLELVRDLQGLGFTLAAIEGYLARIPPDRSPEDLALHRALLAPWLPEQPEMVTR
                     AELDMRAGRCLDDDELAQLVMLGVLRRNGVETWTLTSSDMLSYGLSLLDAELDFDVLL
                     AAKQITERHTDALATELVTLFHDTVLREYRERGRPPELRHRLLALLERLKPITVDGVV
                     TNFQLAINRAIRESVPAEATTAVPKPGASHD"
     gene            1212..>1643
                     /locus_tag="VHH53_11460"
     CDS             1212..>1643
                     /locus_tag="VHH53_11460"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803107.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX2290783.1"
                     /translation="MTEAFLYEALRTPRGKGKPTGALHGVKPISLVTGLIDELRHRHP
                     SLDPARIDDIVLGVVTPVGDQGGDIPRAAALAAGLPDTVAGLQINRFCASGLEAVNLG
                     AMKVRSGWEDLVLTGGVESMSRVPMGSDGGAIAMDPETAYHI"
BASE COUNT          261 a          563 c          556 g          263 t
ORIGIN      
        1 cggcggtgaa gccccacagc gcgaatccct gcagcgcgac ggggaagttg ccgaaaggtc
       61 ccatcccggc gtcggtgccg gtgatcaggc ggactccctg gtcggccagc catcgcaccc
      121 gcccgacgat ctgttgggca ccctcggcac cgaagcgcgc ggccatcgga cgccagttgt
      181 tggcgttggc ggtgcacacc gcgatcccgg ccgccacgat gtcgcgggcg atcttctcat
      241 cgggctggaa ggtccctcct ggtccggaca gccaggtgca gtgctcgatg gtgtcgacac
      301 cagcgtccac cgcggctcgg atgctgctgg tgccatgagc gtgcgctgcg acggtgcgcc
      361 tagcctggca gctcgaccgc cattcggagc cgaatggcag ccggggccac ccatacgtga
      421 cagtggtact gttacaacag tgtcactgtc atatcgggaa gggcgatcgg tggcggagca
      481 gctcaccatc tcggagctgg ccgcgcgcac cgggatcagc gtgcgcacga tccggttcta
      541 caccgggatg ggcctgatcc tgccaccaat ggtgcgcggt cggctgggcc tgtatgacga
      601 acggcacgtg gcccgccttg agctggtccg cgacctgcag ggcttgggtt tcacactggc
      661 agcgatcgag ggctatctcg cgcggatccc gccggaccgc tctcccgagg acctcgcgct
      721 gcaccgagca ctgctcgccc cgtggctgcc cgagcagccc gagatggtga ctcgcgccga
      781 gctcgacatg cgcgccggac gctgtctgga cgacgacgag ctggcccagc tggtgatgtt
      841 aggcgtgctc cggcgcaacg gggtcgagac gtggacgctg accagttccg acatgctgag
      901 ctacgggctg agcctgctcg acgccgagct ggacttcgat gtcctgctgg cggccaagca
      961 gatcaccgag cggcacaccg acgcgctggc caccgaattg gtgacactgt tccacgacac
     1021 cgtgttgcgc gagtaccgcg agcgcggccg gccaccggag ttgcggcacc ggttactggc
     1081 cctgctggag cggctcaagc cgatcaccgt cgacggcgtc gtgaccaatt tccagctcgc
     1141 gatcaatcgc gcgatccgtg agtctgttcc cgccgaggcg acaactgctg tcccgaaacc
     1201 cggagcgagt catgactgaa gcatttctct atgaggcgct acgcaccccg cggggcaaag
     1261 gcaagccaac cggcgcgctg cacggcgtca agcccatctc cctggtcacc gggctgatcg
     1321 acgagctgcg ccaccgccac ccgagcctgg acccggcccg gatcgacgac atcgtgcttg
     1381 gagtggtcac tcccgtcggc gatcagggtg gcgacatccc gcgtgccgcg gcgctggccg
     1441 ccgggctgcc ggacaccgtc gccgggctgc agatcaaccg gttctgcgcc tcgggtctgg
     1501 aagcggtcaa cctcggcgcg atgaaggtcc gctccggctg ggaggacctg gtactcaccg
     1561 gcggcgtcga gtcgatgtcc cgggtgccga tgggctccga cggtggtgcc atcgcgatgg
     1621 acccggaaac cgcctaccac atc
//
LOCUS       DATADN010000883         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1022407, whole genome shotgun sequence.
ACCESSION   DATADN010000883 DATADN010000000
VERSION     DATADN010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022407"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1025)
                     /locus_tag="VHH53_11465"
     CDS             complement(75..1025)
                     /locus_tag="VHH53_11465"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX2290784.1"
                     /translation="MNRELDAAGITDPALRASYTRCRQLNAVHGRTYFLATRLLPPAA
                     RPAIHALYGLARYADEIVDDLADIRPWPQRLAELDTFAAQLAEGLARGSSDHPVLAAP
                     VHTAIRYRIDPQHFTDFMASMRMDRPVEQGGVAGYQTFDELGRYVHGSAAVIGLQVLP
                     VLGTVVAQERAEPHAAALGIAFQITNFLRDVGEDLDRGRVYLPADELAAFGVDRTLLA
                     WCRSTGRSDPRVRRALAHLVAHTRAIYRRAAPGIPMLAPVSRACVATAFRLYGGILDE
                     IEAAGYHILSRRVAVPRHRRLAVALPGLSRALAARTVRAG"
     gene            complement(1022..>1868)
                     /gene="mptB"
                     /locus_tag="VHH53_11470"
     CDS             complement(1022..>1868)
                     /gene="mptB"
                     /locus_tag="VHH53_11470"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEX2290785.1"
                     /translation="NPMTVVHLVGGPHNDLLMIGLLALGTLLVLQRKHVAGIAVVTVA
                     MAVKASAGVALPFLIWIWAAHLPGSRGARLLRAGAGGLAVFVTVFSAVTVLSGVGLGW
                     IPALNAPSLIINWMSLPTAAGQVAWAMTMPLHDLSSTPFIIVFRILGSVVFLGIFARQ
                     WWLAREGGPETVRRAAIALLWSALLAPATLPWYFTWALVLAAALPWRRSPLAWAVVGS
                     TWLVLATYPTGESAFGSVPYHIGMLAIALLAGTALLRRDPLRLRVRTHRTEAVPSRAT
                     GAETA"
BASE COUNT          327 a          644 c          654 g          243 t
ORIGIN      
        1 gcgccgacct gctgcgccag ggcgcccgcg cactcatcga ctccatcgcc cactgatccg
       61 tcgccgggtt gggttcagcc ggctcgcacc gtccgcgctg ccagtgcccg gctcaggcct
      121 ggcagggcga ccgccagccg gcggtggcgt ggtactgcca cccgccggga cagaatgtgg
      181 tacccggccg cctcgatctc gtccaagatg ccgccgtaga gcctgaatgc ggtagccaca
      241 caggcccgcg acaccggggc cagcatcggg atccctggtg ctgcccggcg gtagatcgca
      301 cgggtatggg ccaccaggtg agccagtgcc cggcgcaccc gggggtcgga gcgtccggtg
      361 ctgcgacacc atgccagcaa cgtccgatcc accccgaatg cagccagctc gtcagcgggc
      421 aggtagaccc gcccgcggtc gagatcctcc ccgacgtcgc ggaggaaatt ggtgatctgg
      481 aaggcgatgc ccagtgccgc ggcgtgcggt tcggcccgct cctgggcgac cacggtgccc
      541 agtaccggca gcacctgcag tccgatgacc gccgccgagc catgcacata gcggcccagc
      601 tcgtcgaagg tctggtaacc ggccacgcca ccctgttcca ccggccggtc catccgcatc
      661 gaggccatga aatccgtgaa gtgctgcgga tcgatccggt agcggatcgc ggtgtgcacc
      721 ggcgcggcca gcaccgggtg gtcggaactg cctcgcgcca acccctcggc cagctgggca
      781 gcgaaagtgt ccagctctgc aagacgctgc ggccaaggcc ggatatcggc caggtcgtcg
      841 acgatctcgt cggcgtagcg ggccagccca tagagggcat ggatcgccgg ccgcgccgca
      901 ggtggtagca gccgggtcgc caggaagtaa gtgcgaccgt gcaccgcgtt gagctgccgg
      961 caacgggtat acgaggcccg cagcgcaggg tcggtgatgc ctgccgcgtc aagctcgcgg
     1021 ttcatgcggt ttctgcaccc gttgcgcggg acggcacggc ctccgtgcgg tgcgtgcgga
     1081 cacgcaggcg cagcgggtcg cggcgcagca gtgcggtgcc cgctagcaac gcaatcgcca
     1141 gcatcccgat gtggtagggc acggagccga acgcgctctc tccggtgggg taagtggcca
     1201 gcaccagcca ggtggatcca acgacggccc acgctagcgg acttcgccgc cagggcaagg
     1261 ctgcggcgag gaccagggcc caggtgaagt accagggcag cgtggcgggc gccagcagcg
     1321 cggaccacag cagcgcgatc gccgcgcgac ggacggtctc aggccctccc tccctggcca
     1381 accaccactg ccgggcgaag atacccagaa agaccacaga gcccaggatc ctgaagacga
     1441 tgatgaacgg ggtgctgctg agatcatgca gcggcatggt catggcccag gcgacctgac
     1501 ccgcggcggt aggaagcgac atccagttga tgatcaggga cggtgcgttc agggcgggga
     1561 tccagccgag accgaccccg gacagcaccg tgacagcgct gaacaccgtg acgaacacgg
     1621 ccaggccgcc ggcgccggcc cgcagcagcc gagcgccgcg gctaccgggg aggtgggcag
     1681 cccagatcca gatcaggaaa ggcaaggcga ccccggctga cgccttgact gccatcgcga
     1741 cggtgaccac cgcgattccg gcgacgtgct tgcgttgcag caccagcagc gtgcccaagg
     1801 ccagcaggcc gatcatcagc aggtcgttgt gcggtccgcc gaccagatgc acgacggtca
     1861 ttgggttg
//
LOCUS       DATADN010000884         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1472473, whole genome shotgun sequence.
ACCESSION   DATADN010000884 DATADN010000000
VERSION     DATADN010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472473"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VHH53_11475"
     CDS             complement(<1..356)
                     /locus_tag="VHH53_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2290786.1"
                     /translation="MNRDTSGIVAVLVTSVLWGTTGVAATFAPDAGPLAIGAAALGIG
                     GVLQAVIAVPSISRSRQALREHARLVTMGALALAIYPLANYSSMHLGGVAVGTIVSGA
                     ATPLASGILERVLERRP"
     gene            complement(353..946)
                     /locus_tag="VHH53_11480"
     CDS             complement(353..946)
                     /locus_tag="VHH53_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005449686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2290787.1"
                     /translation="MTSQEVGSGRRHGPARARILDAAAQRFYADGIAATGIDTITASA
                     GVAKMSLYNNFASKAALVEAYINQRHEEWLDLYRVRITDSATAQERVLAVFDAYIDHA
                     AAAYDQGFRGCGLLNAAAELAADDPGRIAVRRHKEEVERILLEQLTALTDTAAAGRLA
                     EHLSFLLEGAMARAGLDGESTRLHHARAFAAQMLAAL"
BASE COUNT          263 a          529 c          507 g          231 t
ORIGIN      
        1 ggccggcgtt ccaggacccg ttccaggatg ccggaggcca ggggcgtggc cgctccggag
       61 acgatcgtcc cgaccgcaac tccgcccaga tgcatcgagc tgtagttcgc cagcggatag
      121 atcgccaacg ccagcgcgcc catggtcacc agcctcgcgt gctcccgcag cgcctgtcgg
      181 gagcggctga tggacggcac ggcgatcacc gcctgcagca caccgccgat gcccagtgcg
      241 gccgccccga tcgccagggg gccggcgtca ggggcgaagg ttgctgcgac gccagtggtg
      301 ccccacagaa ccgaggtgac taggaccgcg acaatgccgc tggtatcccg gttcacagcg
      361 cggccaacat ctgggcggcg aacgcgcgag catggtgcag gcgcgtcgac tcgccgtcca
      421 gcccggcacg ggccatcgcc ccctccagca gaaagctgag gtgctccgcc aggcggcccg
      481 ccgccgccgt gtcggtcaac gcggtcaact gctcgaggag aatgcgttcc acctcctcct
      541 tgtggcggcg caccgcaatc cgcccgggat cgtcggcagc gagttcggcg gcggcgttga
      601 gcaggccaca gccccggaaa ccctggtcgt aggcagccgc ggcgtgatcg atgtaggcgt
      661 cgaacaccgc gagcacccgc tcctgggctg ttgcgctgtc cgtgatccga actcgataca
      721 ggtcgagcca ctcctcgtga cgctggttga tgtaggcctc caccagcgcg gccttcgagg
      781 cgaagttgtt gtacaggctc atcttcgcca caccggcgct agcggtgatc gtgtcgatgc
      841 cggtggcggc gatgccgtcg gcgtagaagc gctgcgcggc agcatcgagg attcgtgccc
      901 gcgcgggtcc gtggcgccgg cccgagccga cttcctgtga cgtcacgaac tgacctcccg
      961 aaaagtggat agaccagtct acctagtccc ccaggcggag gccgaacgca aggccgccca
     1021 cgccgaagcc atcaacgacg cggcaatgat gcggctgatg gcggctctcc tgactcaggc
     1081 cgctcccctg agggaagaac ggcctgagtc aggagggcgg agctgaggga actgaacccc
     1141 tgacccccac tctgccaggg cggctgaaag gggtcggcgc aggttcgtca gagtccgttc
     1201 tcgcaggtca gcggcatgcg cggttctcag cgaaccgtca cgaatgaccc tgaacgccga
     1261 ccttaggcca ccaccgtggc accacgcgtt tgcggcctga tcgccatcga ccatcgccat
     1321 gtcgggggcc gttcatgatc ggttggccta ctggcgagcc gactcaaacc atgcaactga
     1381 cagttccctg gcacggggtt cgtggacgta cgtcgtcgta cgaacagcca ggtcggacgg
     1441 ctgatgcgga cgtccgcgca tggctgcgga cacctgtgaa cgccccgcgg tggcacacac
     1501 gctggcacac agcatcaccc tcacctgtcg
//
LOCUS       DATADN010000885         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_250974, whole genome shotgun sequence.
ACCESSION   DATADN010000885 DATADN010000000
VERSION     DATADN010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250974"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHH53_11485"
     CDS             complement(<1..651)
                     /locus_tag="VHH53_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HEX2290788.1"
                     /translation="MDVEQIPLHFGQHAIDALTHVNLSAVRTDGSCLWIAGDETATVE
                     RLTVEGEGYGKHVTFRLADVLTLPSGPEDEVDVEGLARHGPYLWAVGSHSSRRKRIKA
                     KHEGDKAVRRLAQVTDEPSRRIVARLAIADGPDGLPTIVAEAPGGYTSALLPGGITGT
                     LADDPHLAPFLAIPGKDNGFDVEGVAVRGESLYLGLRGPVLRGWAVVIALAPQLDPA"
     gene            complement(754..1266)
                     /locus_tag="VHH53_11490"
     CDS             complement(754..1266)
                     /locus_tag="VHH53_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2290789.1"
                     /translation="MPSHHPLPFDPIERAAQLWQDRIGPAETMAAVTNVMRVQQILQS
                     AVDAALRPHGLTFARYEALMLLLFSRQGSLPMKVMGQRLQLHPTSVTNIVDRLQADQL
                     VERSGHPTDRRTTLVTITEAGRQRQKVATVALTDIDFGLVGLDAEHTEQLTALLTRVR
                     RAAGDFTDPA"
     gene            1341..1634
                     /locus_tag="VHH53_11495"
     CDS             1341..1634
                     /locus_tag="VHH53_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEX2290790.1"
                     /translation="MIVAFSVTPAGGGSDSVSAAVAEAVRVVADSGLPYELNAMFTLV
                     EGEWDEVMDVVRRAVDAASVGATRTSLVLKADIRPGYTGQLRAKVDRVKAHCK"
BASE COUNT          275 a          544 c          588 g          280 t
ORIGIN      
        1 cgctgggtcg agctggggtg ccagcgctat taccacggcc cagccgcgca gcacgggtcc
       61 ccgtaatccc agatacagcg attcgccccg aactgcgaca ccttcgacgt cgaagccgtt
      121 gtccttgccg ggaatcgcca ggaacggggc caggtgaggg tcgtcggcca gtgttccggt
      181 gatcccgccg ggcagcagcg cactggtgta cccgccaggc gcctcggcga cgatcgtcgg
      241 gaggccgtcc gggccatccg cgatggccag ccgcgccacg atccggcggc tgggctcgtc
      301 ggtcacctgc gccaggcgcc gcaccgcctt gtccccctcg tgcttggcct tgatccgctt
      361 gcgccggctg ctgtgcgaac cgaccgccca caggtagggg ccgtggcggg cgagtccctc
      421 gacgtccacc tcgtcctccg ggccactggg caacgtgagg acatcggcga ggcggaaggt
      481 gacgtgcttg ccgtaaccct cgccctcgac ggtgagccgt tcgacggtcg cggtctcatc
      541 cccggcgatc cacaggcagg agccgtcggt tcggacggcg gagaggttga cgtgggtcag
      601 cgcgtcaatg gcgtgttggc cgaagtgcag cggaatctgc tcgacgtcca cggtgcccga
      661 cctcctcgcc acaaccaaga ttagcgccgt ccgtgcaccg cagaaggaca caccgtgcac
      721 ggcggcagga cacgccggga aggggcgagc gggtcaggca gggtcggtga agtcccctgc
      781 ggcgcggcgt acccgggtga gcaacgcggt gagctgttcg gtgtgttcgg cgtccagccc
      841 gacaagcccg aagtcgatgt cggtcagtgc cacggtggcc accttctggc gctgccgtcc
      901 cgcttcggtg atggtgacca gggtggtgcg tcgatcggtg ggatggccgc tgcgctcaac
      961 cagttgatcg gcctgcaacc ggtcgacaat gttggtcaca ctggtcgggt ggagttggag
     1021 ccgctggccc atcaccttca tcggtaggct gccctggcgg gaaaacaaca gcaacatcaa
     1081 tgcctcatac cgggcgaagg tcaggccgtg cggccgcagc gccgcatcca ccgcggactg
     1141 caggatctgc tgcacgcgca tcacgttggt gaccgcggcc atcgtctcag caggcccgat
     1201 gcgatcttgc catagctgcg cagctcgctc gatcggatcg aaaggcagcg ggtgatgcga
     1261 cggcatggcg taaccgtagc ggctgtccgg cgggtttccc ggcagccacc gtgctactcc
     1321 gtatagggca agctggacga atgatcgtgg cattcagcgt cactcccgcc ggtggtggat
     1381 cggacagcgt cagtgccgcc gtggccgagg cggtgcgggt ggtcgcagac tccggactgc
     1441 cctacgaact caacgccatg ttcaccctcg tggaggggga gtgggacgag gtcatggacg
     1501 tggtgcgccg ggcggtcgac gccgccagcg tgggtgctac ccgcaccagt ctggtgctca
     1561 aggccgatat tcgtcccggg tacaccggcc aactgcgcgc caaggtcgat cgggtcaagg
     1621 ctcactgtaa ataaagtgcg ggagagctag taggacgtcc tactattgga gacatgactc
     1681 gctacga
//
LOCUS       DATADN010000886         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_251126, whole genome shotgun sequence.
ACCESSION   DATADN010000886 DATADN010000000
VERSION     DATADN010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251126"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VHH53_11500"
     CDS             <1..602
                     /locus_tag="VHH53_11500"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2290791.1"
                     /translation="FTAGLPRDSQQLISEQLAAITGSSGGALTIGLVVSLLLALWSAS
                     SGTSSLMTAVNLAYDEKEKRGFLKLRGVALLLTFGAVVFLVLTLALIAVVPVVLQAVP
                     LGPVGTVLAQVLRWTLLFVLVVVGLAVLYRVAPDRNSPKLRWVALGSMVAAVLWLLGS
                     AGFSLYVNSFGNYNKTYGALAGVIVLLLRLLLTCYVVLL"
     gene            718..1224
                     /locus_tag="VHH53_11505"
     CDS             718..1224
                     /locus_tag="VHH53_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX2290792.1"
                     /translation="MGNAGHAQRSKLAEGIAEIVDPAGLTVAVAESLTGGMVASALAE
                     APGSSAWFRGAVVAYASEVKHQLLDVPAGPVVSAEAAASMADGVRRLLGADIAVASTG
                     AGGPGGQDGQPPGTVFLALSDGLHTEVEHYFFDDDPAEVCDRTAAEALQMLLTHLSKL
                     PEDQLRPR"
     gene            1267..1515
                     /locus_tag="VHH53_11510"
     CDS             1267..1515
                     /locus_tag="VHH53_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290793.1"
                     /translation="MTEEVGTVQYEVLIEGKIHPWAKDTISVADIREAGNLPANTAVV
                     EDNLRDGSKRTLGEDETLHPAKLEEGKPPTKRVNFRRA"
     gene            complement(1599..>1828)
                     /locus_tag="VHH53_11515"
     CDS             complement(1599..>1828)
                     /locus_tag="VHH53_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290794.1"
                     /translation="ATRLLIRRVRLAPEQISADPRARRRRTLHPDHWALPINELADTV
                     TIYGYSLIVSNLAVSAGEQAIAVERWYRRHS"
BASE COUNT          311 a          562 c          640 g          315 t
ORIGIN      
        1 gcttcacggc ggggctgccg cgagactccc agcagctgat ctccgagcaa ctggccgcta
       61 tcacgggcag tagcggtggt gcgctgacaa tcgggttggt ggtctcgctg ttgctcgcgc
      121 tgtggagcgc gtcaagtggg acatcgagcc tgatgacagc agtgaacctc gcctacgacg
      181 agaaggagaa gcgcggtttc ctgaaactcc gtggggtcgc gctgctgctc accttcggcg
      241 cggtggtgtt cctggtattg accctggcct tgatcgcggt ggtgccggtg gtgttgcaag
      301 cagtgccgct gggcccggtc ggcacggttc tcgcgcaagt gttgcggtgg acgttgctct
      361 ttgtcctggt cgttgtcggg ctggcggtgc tgtatcgggt ggctcctgac cgcaactcac
      421 cgaagttgcg ctgggtggcc ctgggcagca tggtggcggc agtgctgtgg ttgctcggaa
      481 gcgcggggtt cagcctctac gtcaactcat tcggcaacta caacaagacc tatggcgcgc
      541 tggccggcgt gatcgtgctg ctgctacggc tgctgttgac ttgttacgtc gtgctcctct
      601 gagcagagat caactccgag gccgagcgcc agaccgcgcg cgacaccacc atcggagaac
      661 cgcggccaat ggggcagcgc ggggcagtgg tcgccgacac catgccggga catcgcagtg
      721 ggtaacgctg gtcacgcgca gcggtcgaaa ttggcagagg gcatcgctga gatcgttgac
      781 ccggcggggc tcactgttgc ggtggccgag tcgctgaccg gcgggatggt ggccagcgcg
      841 ctagccgagg cgcccgggtc gagcgcctgg tttcggggag cagtggtggc ctatgcgagc
      901 gaggtcaaac accagcttct cgacgttcct gctgggccgg tggtcagcgc ggaagccgcc
      961 gcctcgatgg ccgatggcgt ccggcggctg ctcggagccg atatcgccgt cgcgtcgacc
     1021 ggggcgggcg gcccaggtgg gcaggacggc cagccgcccg gcaccgtgtt cctcgcgctg
     1081 tccgacggcc tgcacaccga ggtcgagcac tacttcttcg acgacgatcc agccgaagta
     1141 tgtgaccgaa cggccgctga ggcgctccag atgctcctca cacacctctc caagctcccg
     1201 gaagaccaac ttcgtccgcg ctgaaccgag aatcagtgtc aacatcaaca gagaggttga
     1261 tcagacatga ccgaagaggt aggcaccgtt cagtatgagg tgctgataga gggaaagatc
     1321 catccatggg ccaaggacac catctcggtg gctgacattc gggaggccgg caacctgccc
     1381 gcgaacacgg ccgtcgtcga ggacaacttg cgcgacggct ccaaacgcac gctgggcgaa
     1441 gacgagacgc tgcatccggc caagctcgag gagggcaagc cgcccaccaa gcgggtcaac
     1501 ttcaggcgag cctgatcggc cgacggcgac agctccacgc tcgccctcgt tcacgccgac
     1561 catcagccga gctgtgaccc cgggttacag ctcggccact atgaatgtcg ccggtaccag
     1621 cgctccaccg cgatggcctg ctcgccggcg gagacagcca ggttggacac gataagcgag
     1681 tagccgtaga tggtcacggt gtcagcgagt tcgttgatcg gcagggccca atggtcgggg
     1741 tgcagggtgc gacgccgccg ggcgcgcggg tcagcactga tttgctcggg tgccaggcgg
     1801 acccggcgga tcagcagccg ggtcgccg
//
LOCUS       DATADN010000887         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_566425, whole genome shotgun sequence.
ACCESSION   DATADN010000887 DATADN010000000
VERSION     DATADN010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566425"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VHH53_11520"
     CDS             complement(<1..367)
                     /locus_tag="VHH53_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290795.1"
                     /translation="MATTGRPRWAATGMAERSDVLGALERLLDDAGEGRGGALFIVGP
                     AGLGKTTVLEHAITVARSQFAVGVGRGDQVEAVLPFGLIGQALDQLLEGKLPGGGLML
                     GTDGGEASDISPQARFYGIL"
     gene            515..1132
                     /locus_tag="VHH53_11525"
     CDS             515..1132
                     /locus_tag="VHH53_11525"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX2290796.1"
                     /translation="MRLVLASASPARLAVLRAAGIDPVVRVSAVDEDALARKLPGATP
                     AQLVTALAQAKADAVVVGIGAEHPDTVVVGCDSMLHIGGELVGKPRCADVALRRWQAM
                     ADGTGELLTGHAVVRLHQGVVTATASGAQTTLVRFGTLSAEEISAYVDTGEPLQVAGA
                     FTLDGRGGWFVDGIDGDPSSVIGISLPLTRRLLAEVGVPVITLWS"
     gene            1186..1623
                     /locus_tag="VHH53_11530"
     CDS             1186..1623
                     /locus_tag="VHH53_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX2290797.1"
                     /translation="MSTPATLPPRLAELAEEFEDVSPNERLQLLLELSGELPDVPPHV
                     ARESMQRVPECQSPLSLSVEVGDDQRVHLHFDAPPEAPTTRGLAAILYTGLDGEPVPT
                     VLATPDDFFLHFGLAEAVSPLRLRGVAAMLARIKRTVRQTEVP"
     gene            1654..2574
                     /locus_tag="VHH53_11535"
     CDS             1654..2574
                     /locus_tag="VHH53_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290798.1"
                     /translation="MPAPRYTLIRGEFYIRYPDLPRNGPEPDGDTINFLPDNDDLVRG
                     LPRFDNIGPDRRHLGTYGTRFEGIDTLETHFENQHQNLHFAQAARDRMLQIMGFGKVQ
                     FWPDRPNKIESAERHPIPGYLLANGIERNGRVLALVFPGSPTLDAADGDPVFVDLLAL
                     EASVNAELVRSSLAYAELYSTMPIGLIHRMRQLVTAARQAGGGLWPHEHISTTARVQL
                     TSLADLSTLIMFPKLYRRLVRYFQAGHTDLAAFDTWIRADPDRDDRVLLPTGELGHLH
                     DLYDVDPDGIGLRYLPEELTFISGPTVAPG"
     gene            2636..>2768
                     /locus_tag="VHH53_11540"
     CDS             2636..>2768
                     /locus_tag="VHH53_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2290799.1"
                     /translation="MSDPTTLRKVLIANRGEIAVRIIRACRDAGLNSVAVYADDDRDA
                     "
BASE COUNT          464 a          961 c          891 g          452 t
ORIGIN      
        1 ggagaatgcc gtagaaacgt gcctgcgggg agatgtcgga ggcttcgcca ccatcggtac
       61 ccagcatcag accgccaccg gggagctttc cctcgagaag ttgatcgaga gcctggccga
      121 tcaaaccgaa cggcagcacc gcctcgacct ggtcgccacg tccaacaccg accgcgaact
      181 gggatctcgc cacggtgatc gcatgctcga gcacggtggt cttgcccaat ccggccggcc
      241 cgacgatgaa cagcgctcca ccccggccct caccggcgtc gtcgagcagc cgctccagcg
      301 cgcccagcac gtcactacgt tcggccattc cggtagcagc ccaccggggc cgtcccgtcg
      361 tggccatgaa cctgccctct ctgttgcccc tctgccggcc aacaccggca acacagagga
      421 ggaggacacg ccacatccgg taagtacgtc atgctcaatt cccgattgag cgaacctgtg
      481 cggcgatgca gacgcctccg tacagtgcgt caccgtgcgt ctggtgctcg cgtcggcatc
      541 tccagctcgg ctcgcggtgc tccgcgccgc cggaatcgat ccggtggtga gagtgtccgc
      601 agtggacgag gatgcgctgg ccaggaagtt gcccggtgcg acgccggctc agttggtcac
      661 cgcgttggcg caggcgaagg cagacgcggt ggtggtcggc atcggcgccg agcaccccga
      721 cacggtggtt gtggggtgtg actcgatgct gcacatcggt ggcgagctgg tcggtaagcc
      781 ccggtgtgcc gacgtcgcgc tgcggcgctg gcaggcgatg gctgatggca ccggcgagtt
      841 gctcaccggg cacgccgtgg tacggctgca ccagggcgtt gtcaccgcca ccgccagcgg
      901 cgcccaaacc acgctggttc gcttcggcac cctgtcggcc gaggagatat cggcctacgt
      961 tgacactgga gaaccgctgc aggtggccgg cgctttcacc ctcgacggcc gcggcggctg
     1021 gttcgtcgac gggatcgacg gtgacccgtc cagtgtcatc ggcatcagcc tgccactgac
     1081 ccggcggctg cttgccgagg tcggggtgcc ggtgatcaca ctgtggtctt gatcggcgca
     1141 gcacggtcgc cgacggtagg tatccgacga ggagaggagg ccacgatgag tacgccggcc
     1201 accttgccgc cgcggctggc cgagctggcg gaggagttcg aggacgtcag ccccaacgag
     1261 cggctgcagc tgctgctgga gctgtccggc gagctgcctg acgtgccgcc gcacgtggcg
     1321 cgcgagtcga tgcagcgggt gcctgagtgc cagtctcccc tgtcgttgtc cgtggaagtg
     1381 ggtgacgacc agcgggtgca cctgcacttc gacgcaccgc ccgaagcccc cacgacccgc
     1441 gggctcgcgg cgatcctcta caccgggctg gatggcgagc cggtgcccac cgtgttggcc
     1501 acaccggatg actttttcct gcatttcggt ctggccgagg cggtcagccc gctgcggctg
     1561 cgtggcgtgg ccgcgatgct ggcccggatc aagcggaccg tacgccaaac tgaggtgccg
     1621 tagcgtcgat cgcgacacac ccggaaggat ctgatgccag caccccgcta caccctgatc
     1681 cgcggtgagt tctacatccg ctatccagat cttccccgca atgggccaga gcccgacggc
     1741 gacacgatca acttcttgcc ggacaacgac gacctggtgc gcggcttgcc tcgcttcgac
     1801 aacatcgggc ctgaccgccg tcacctcggc acgtacggca cccggttcga aggcatcgac
     1861 accctcgaga cccacttcga gaaccagcac cagaatctgc acttcgcgca ggccgcgagg
     1921 gatcggatgc tacagatcat ggggtttggc aaggtgcagt tctggccgga tcggccaaac
     1981 aagatcgaaa gtgcggagcg ccatcctata ccgggctatc tgctggctaa cggcatcgaa
     2041 cgcaacggcc gcgtgctcgc cctggtattt cccggctcgc ccaccctgga cgccgccgac
     2101 ggcgacccgg tgttcgtcga tctcctagcg ctggaagcca gcgtgaacgc cgaactcgtg
     2161 cgcagcagcc tggcctacgc cgagctctac tccaccatgc cgataggcct catccaccgg
     2221 atgcgccagc tggtcacggc cgcccgccag gccggtggcg gactctggcc acacgagcac
     2281 atcagcacca cggcccgggt gcagctgaca agcctggccg acctctccac gttgatcatg
     2341 tttccgaagc tctaccgccg tctggtgagg tacttccagg ccggacacac tgacctggct
     2401 gccttcgaca cgtggattcg cgccgatccg gaccgcgacg accgggtgct cctgcccacc
     2461 ggcgaactcg gtcacctgca cgacctttat gacgtggatc cggacgggat cgggttgcga
     2521 tacctgcccg aggagctgac gttcatatcc ggccctaccg ttgcaccggg ctgaccacgc
     2581 atcggcccta gactgcggcg aatacgacct caccaccgcg gagaaaccgg tgcctctgtc
     2641 tgacccgacg accctgcgca aggtgctcat cgccaaccgc ggtgagatcg ccgtccggat
     2701 tatccgggcc tgccgggatg ccgggctgaa cagcgtcgcg gtctatgccg acgacgaccg
     2761 cgacgcgc
//
LOCUS       DATADN010000888         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1304453, whole genome shotgun sequence.
ACCESSION   DATADN010000888 DATADN010000000
VERSION     DATADN010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304453"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="VHH53_11545"
     CDS             complement(<1..1512)
                     /locus_tag="VHH53_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX2290800.1"
                     /translation="MRARRFGTVLAVGLLLGAGLITGVAAPAQAASRVLPDAKTPVVQ
                     GYGGAVVSDTAESTQAGLDVLGKGGTAADAAVAVAATLGVTDPYVAGIGGGGYFVYYD
                     ARTHRVSTIDRRETAPAAAGPSMSIDPATGQPLPVPDAVTSGLSVGVPGTLATWERAL
                     ARWGRFGLAENLRPAEEVAERGFVVDQTFHEQTRQNAKRFAQFGSTARLFLPGGTLPA
                     VGSVLRNPELARTYREIGERGIGELYGGPIGTDLVNAVQHPPMATGATLVARPGLMTL
                     RDLRAYDALDADPTHVSYRGLDVYGMASSSSGGITDGEALNILGEVDLSAMSRVQALH
                     HYLEASRLAFADRNRYLGDPRYVEVPQQQLLSQSFARQRACLIDPAKAATSPVAPGDP
                     SVQSHRCMPVGAAATVPDRGNHTNHFVVADSAGNVVSYTNTIEQLGGNGIVVPGRGFL
                     LNNELTDFNFSPTQGSAPDPNLPAAGKRPRSSMSPTIVLRDGRPYLAVGSPGGA"
BASE COUNT          261 a          565 c          552 g          245 t
ORIGIN      
        1 ggcgccaccg ggcgagccga ccgcgaggta cggccggccg tcgcgcagca cgatggtcgg
       61 cgacatgctc gagcggggcc gcttgccagc cgccggtaag ttaggatcgg gcgcgctgcc
      121 ctgcgtcggc gagaaattga agtcagtcag ctcgttgttc aggaggaagc cccggccggg
      181 aaccacgatc ccgttgccac ctaactgctc gatcgtattg gtgtacgaca cgacgttgcc
      241 cgcgctatca gcgaccacga agtggttggt gtggttgccc cggtcaggca ccgtggcggc
      301 cgcaccgacc ggcatacagc gatgcgattg gacggacggg tcacccgggg cgaccgggct
      361 ggtggcagcc ttcgccggat cgatgaggca ggcacgctgg cgggcaaagg attgcgagag
      421 caattgctgc tggggcacct ccacgtaccg ggggtcgccg aggtagcggt tgcgatcggc
      481 gaacgcgagc cggctggcct ccaggtagtg gtgcagcgcc tgcaccctgc tcatcgcgga
      541 caggtcgacc tctcccagga tgttgagcgc ttcgccgtcg gtgatgccgc cactggacga
      601 cgaagccatc ccgtacacgt cgaggcctcg gtagctcacg tgcgtcgggt cggcgtccaa
      661 cgcgtcgtag gcccgcaagt cccgcagcgt catcaggccg ggccgggcca ccagggtggc
      721 gccagtagcc atcggcgggt gctgcacggc gttgaccagg tccgtgccga tcggaccgcc
      781 gtagagttca ccgatcccac gctcgccgat ctcccggtag gtccgcgcca gctcgggatt
      841 gcgcagcacc gagcccaccg cgggcagcgt gccgccgggc aggaacagcc tcgctgtgga
      901 gccgaactgg gcgaagcgct tggcgttctg ccttgtctgc tcgtgaaacg tctggtcaac
      961 cacgaagccg cgctcggcga cttcctcggc gggtcgcaga ttctcggcga gcccgaagcg
     1021 accccatcgc gccagtgccc gctcccaggt ggccagagtg ccaggtaccc cgacggatag
     1081 cccgctggtc accgcgtccg gcaccggcag tggctgcccg gtcgccgggt cgatggacat
     1141 tgacggtccg gcggcggccg gcgcggtctc tcgcctgtcg atcgtggaca cccgatgggt
     1201 acgcgcgtcg tagtagacga agtacccgcc accgccgata ccggcaacgt aggggtccgt
     1261 cacgccaagc gtggcggcca ccgcgactgc agcgtcagcg gcggtgccgc ccttgcccaa
     1321 cacatcaagc ccagcctgtg tcgactccgc ggtgtccgag accaccgcgc cgccgtagcc
     1381 ctgtaccact ggtgtcttgg cgtccgggag tactcgcgac gcggcctgcg cgggagctgc
     1441 gacccccgtg atcaggcctg ccccgagcag caacccgacg gcaaggacgg tgccgaaccg
     1501 acgtgcacgc atccgcgtcc cccaacactt gcgtcacaac actcgacatt ccagtctggg
     1561 tttacccgca cagcgatcac accgccatcc ttgggacgca ccaggtccga agcggatggt
     1621 cac
//
LOCUS       DATADN010000889         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_713277, whole genome shotgun sequence.
ACCESSION   DATADN010000889 DATADN010000000
VERSION     DATADN010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713277"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..388)
                     /locus_tag="VHH53_11550"
                     /pseudo
     CDS             complement(105..388)
                     /locus_tag="VHH53_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(532..1777)
                     /locus_tag="VHH53_11555"
                     /pseudo
     CDS             complement(532..1777)
                     /locus_tag="VHH53_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072274.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          349 a          702 c          801 g          437 t
ORIGIN      
        1 cactacccct ccatcatctg cacagcaccg catcagaaag atctcctaca gcaaccctcc
       61 cttgcgttcg tggcacactc gcatactgct cgctgacgtg atacgcggcg ggcggctgcc
      121 agatcgcgct tctgctgacc aggacgtcga tgacctggcc gaactgatcg atcgcccggt
      181 acaggtagac ccatcgcccc gagactttga cataggtttc atcgacgaac caccgatcgc
      241 cacagctgtg ccggcagggt cgtgccgcat cgatgagcag cggagtgaac cgttgcaccc
      301 accggtacac gcttacgtga tcgaccgtga tgccccgctc ggccagcagt tcctcgacgt
      361 cgcgatagga ctgtgaggat ctctggtgcc gttcccgggc cggggtctga cccgcggtat
      421 gactgacctc ggtgtcttgg gtttgatctg tcgacccttg gcttggggtg gtgcttgctg
      481 tcgccggacc ggcgtggtgt gtcccgatag acctccgatc agtcgtagtt gtcaagctgg
      541 ggctggttgg gattgcgttg agggtggtcg gaacgccgtg gtttcgtcgt aggtttggcc
      601 ggttagcagg cagtggtgaa ggcagccgag gaggcggttg aacaggttgc gcagggctgc
      661 ggtgttccgg tccccgagat cgcggcggtg gtggtagtgg gctgctgctc ccgcggattt
      721 ggatgaggcg acaaatgccc agatctagcc ggtggcggcc agtcgattgt ttttgatctt
      781 ccggtgggtg atggagatgc tgcgtccgga ggcccgggtg atgggggcgg agccggcgta
      841 ggccttgagc gcccgggcgt cggcgaagcg ggtgcggtcg tcgccgattt cggcgagcat
      901 ccgggcgccg gtcaggtcgc ccaggccggg gaaactggtg atgatctggt agtcgggatg
      961 tgtgacgaag gcttcggtgg tggcgtcgcc gagctgctcg acgctgtggc actcggtgtc
     1021 cagcgtggcc agcaggcgca gggcgcgggc gcctatcgcc ttttccccgg tcggttcgtg
     1081 gcgcagatgc gggcgacgca gggcctggtg caccgtggcg gcaaggtggt cgattccgcg
     1141 tttgcggcca ccgcgacgca acgccgcggc gatcttggcc ttggtcagtg tggcggcgtg
     1201 agccggggtg ggcgcgatgg ccagcacggc gcgagcatcc gcactggcca ggttagtagc
     1261 gttggctgcg gcgaaggcct ccaggaaggc tgggtagtac tcgcgtagca gcgagcgcag
     1321 ctcgttggac gccttggtgc gtcgccaggt cgcgtcctga tgtgcccggg cgagcaccgt
     1381 gatcgccggt ggctagctcg gtgtcggcgg gcagcggtcg gtgcaggtga gcgtcggtgc
     1441 gcaggatgtt cgccaacgtc accgcgtcgg cgtggtcgga ctttttccgc gacgtgcagt
     1501 gccgttgccg gtaacgggcc accgccatcg ggttgatggg gtaaaccttg cggccactgg
     1561 cgcgcagtgc cgcgaccagc agcccgcgcg gggtctcgat cgccaccggg atcgggtcct
     1621 gggcgctgtc gccggcctgg gccagcatca tggtcagttc cgcgaacccc tcgatggttt
     1681 cggtgatccg ccgcttggcc accaaccggc cttgggcgtt gaccacggcg atgtcgtgct
     1741 gcccttgtgc ccagtcgatc ccgcagaaca cgttcaagtg cgtgtgctcc ctccgctggt
     1801 gttgtctctg ctggtcagag acctagacga gaacgcgcag cgccctaatg acgaggctct
     1861 gtggccttcc ctccgatgag ctgttcgcga tcccagcaat ccacgcggtt ccggtcttga
     1921 cggtgagagc tcgtcggctc ccgggtagtg aggtgtccac cgcgcagacg gactcaaacc
     1981 acgaacaaca tcgctgatca aggtgtgatg gtggcccgga cacgccgagg acgccggtct
     2041 atcgggagag ctcgtagtcc ctgggccttg aaggcgtcca cccagactcc gggccgccac
     2101 cacaacgtgt cccaccgtcg acacggtccc caccccccgc ctcagggccg tggagatacc
     2161 gcgctgatgt cagcagaaca cgagctaatc aacaacccga caacacccat gccggtctga
     2221 cccaagctcc ccgcagacca caactgcggc aggcatccgg ggctatcgac gggtcgttcg
     2281 ggagtcata
//
LOCUS       DATADN010000890         4712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_251583, whole genome shotgun sequence.
ACCESSION   DATADN010000890 DATADN010000000
VERSION     DATADN010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251583"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4712
                     /locus_tag="VHH53_11560"
     CDS             <1..>4712
                     /locus_tag="VHH53_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX2290801.1"
                     /translation="VRDRVVEHDQVGEWQQIYEGLYAGSGSVVFGQDFTGWNSSYDGH
                     RIPLPQMQEWREATVARIRSLHPRRVLEVGVGTGLLLSRLASSCDTYWATDFSASAID
                     ALAGQIDQDPELAARVVLRAQPAHDTEGLPAGLFDTVILNSVVQYFPSIDYLVEVLTG
                     MLGLVAPGGAVFIGDVRNLRLLRPLATAVQLHRAAPSADLAVLRRAVEQAVLVEKELL
                     VDPEFFPALQDRLTDLGGVDIQIKRGRYHNELTRYRYDVVLHKHPITPLSLGDAPQLG
                     WGHQISDVRALGEYLTTARPARLRVSGVPNSRVTHETALAQGLQASPLADLLDQLHNP
                     HPTPEAVDPEALCELGQRCGYWVGITWSPAALDAMDVTFADTTQAASAVPVGLYTPTS
                     VAGAPLSSWTNNPTAARGTRALINQLREYVRVRLPEYLVPAAFVVLDGLPLTPNGKLD
                     RKALPAPEFGSAGAGRAPKTPQEQLLAELFAEVLGLAAVGVDDDFFDLGGHSLLATRL
                     GARIRATFGVELEVRALFEAPTVARLAVHLHHAGQARLALTRCQRPDVVPLSFAQRRL
                     WFLHQIEGPSPTYNIPLALRLSGNLDRPALQAALRDVMARHESLRTIFPHTVGAPYQL
                     LLDVEAACPRLAVTLTSEPELSEVLAAAARYGFDLATETPVRAELFVVAPDEHVLLLL
                     VHHIAADGWSMDPLSADLARAYTARCQGQTPGWEPLPVQYADYTLWQHQLLGDQADPD
                     SLFAAQLGYWTQALAGLPDRLDLPTDRPRPPVASYRGDYLTIQLEPKLHHGLTDLARR
                     HGASLFMVLQAGLAALLSKLGAGTDIPVGSPIAGRTDQALDQLVGFFVNTLVLRTDVS
                     GNPNFTQLLTRVRETALSAYAHQDVPFEYLVEVLNPTRSLAHHPLFQIMLALQNAPEA
                     HFQLPGLDVNFMPAPTGTAKFDLGLSLWERRGPDGSPQGIDGIVEYATDLFDPTTVQT
                     LLTRWIRLLDAAVTDPDQPISRINLLDAQERHQILVKANNTDHPIPAASLPTLFETQV
                     AATPQAVAVVFGDTTLTYRQLNTKANQLAHTLITQGVGPEQIVALALPRSPELVVAIL
                     AVLKAGAAYLPLDPDYPTARINFILGDAQPALLVTSEQTAGCVPQDAATPQLVIDHPD
                     TVAVLSGCTETDPTDIDRTTPLLPQQPAYVIYTSGSTGTPKGVVVSHVGVSSLAAAQI
                     DRLGIGASSRVLQFASPSFDASFWELCMGLLSGAALVLAPKDQLLPGTPLCALVNRQQ
                     VTHVTLPPSVLTVLPVEDGLPPAVTVVVAGEVCPAGLVTAWAPGRRMINAYGPTETTV
                     CATMSDPLPPATPTPVPIGRPIANTRVYVLDAGLQPTPVGVAGELYVAGAGLARGYLR
                     RSGLTAERFVADPFGPPGARMYRTGDLVCWNADGDLEFVGRVDDQVKVRGFRIEPGEI
                     ETVLAAHPGVARSAVIAREDRPGDKRLVAYVVPTTQDARRPDVLREYVRQRLPKYLVP
                     AAFVMLDGLPLTPNGKLDRTALPAPDLTPTTPSRAPCSLQEQILCELLAEVLGLDWVG
                     VDDDFFALGGDSIISIQL"
BASE COUNT          778 a         1620 c         1483 g          831 t
ORIGIN      
        1 ggtgcgggac cgggtggtgg agcacgatca ggtcggtgag tggcagcaga tctatgaggg
       61 gttgtacgca ggctcgggtt cggtggtgtt cgggcaggac ttcaccggct ggaacagcag
      121 ctatgacggt caccgtattc cgctgccgca gatgcaggag tggcgcgagg cgacggtggc
      181 gcggatccgg tcgctgcacc ctcggcgggt gttggaggtt ggggtgggca ccgggctgtt
      241 gctgtcccga ctggcgtcga gttgcgacac ctattgggcc actgacttct ccgcctccgc
      301 gatcgatgcc ctggccggtc aaatagatca ggatcccgaa ctggccgccc gggttgtgct
      361 gcgtgcccag cccgcgcatg acaccgaggg actgccggcc gggttgttcg acacggtgat
      421 cctcaactct gtcgtgcagt atttccccag catcgattac ctcgtcgagg tgctcaccgg
      481 catgctgggg ctggtcgccc ccggtggggc agtgtttatc ggtgacgtgc gcaacttgcg
      541 gctgctgcgc ccgttggcca ccgcagtgca gttgcaccgg gccgccccgt cggcggacct
      601 ggcggtgctg cgtcgggcgg ttgaacaggc cgtgttggtg gaaaaggaac tgctggtcga
      661 cccggagttc ttccccgcac tgcaagatcg tctcaccgac ctcggtggag tggatatcca
      721 aatcaaacgc ggccggtatc acaacgagct gacccgttac cgctatgacg tcgtactgca
      781 caagcacccg attaccccgc tgtcgcttgg cgacgccccc cagctgggct ggggccacca
      841 gatcagcgac gtgcgcgccc tgggcgagta tctgaccact gcccgtccag cgcggttgcg
      901 ggtcagcggg gtacccaaca gccgcgtcac ccacgaaacc gcgctggcgc aaggattgca
      961 ggccagcccg ctggccgatc tgctcgacca actccacaac ccccacccca ccccagaagc
     1021 ggtcgaccct gaggctttgt gcgagctggg ccagcgatgc ggctactggg taggcatcac
     1081 ctggtcacca gccgcccttg acgccatgga cgtcaccttc gccgacacaa cacaagccgc
     1141 ctcggctgtt cccgtcgggc tgtacacgcc caccagtgtc gcgggggcgc cgctgtcgtc
     1201 gtggacgaac aaccccaccg ctgcccgggg gacccgcgcg ctgatcaacc agctgcgcga
     1261 gtatgtgcgt gtgcggttgc cggagtatct ggtgccggcg gcgtttgtgg tgttagacgg
     1321 gttgccgttg acgccgaatg gcaagttgga ccgcaaggcg ctgccggcac cggagttcgg
     1381 gtcggcgggt gcgggtcggg cacccaaaac cccgcaagaa caactgctcg ccgagttgtt
     1441 cgctgaggtg ctgggcctgg ccgcagtcgg tgtcgatgac gactttttcg acctgggtgg
     1501 gcactcgctg ctggccaccc gcctgggcgc ccggatccgt gccaccttcg gtgtcgagct
     1561 ggaagttcgt gccctattcg aggcccccac cgtggcccgg cttgccgtgc acctgcacca
     1621 cgccgggcag gcgcgcctgg cgttgacccg gtgtcagcgc ccggatgtgg tgccgttgtc
     1681 cttcgcccag cgccggttgt ggttcctgca ccagatagaa ggccccagcc cgacctacaa
     1741 cattcccctt gcgctgcgtt tatccggcaa cctggaccgc cccgcgttac aagccgccct
     1801 gcgtgacgtg atggcccgac acgagagcct gcgcacgatc tttccccata ccgtgggcgc
     1861 gccctaccag ctgctcttag acgtcgaggc agcctgccca cggttggcgg tcaccctgac
     1921 cagcgagccc gagttgtctg aggtgctggc ggctgcggca cgctacgggt ttgaccttgc
     1981 caccgaaaca ccggtgcgcg ccgagctgtt cgtggtggca ccggatgagc atgtgttgtt
     2041 gctgttggtg catcacatcg ccgcggatgg ctggtcaatg gaccccctgt cagccgatct
     2101 ggcccgggcg tatacagccc gctgccaagg ccaaacaccg ggctgggaac cactgcccgt
     2161 gcaatacgcc gactacaccc tgtggcaaca ccagctgctg ggtgaccagg ctgatcccga
     2221 cagcctattc gccgcccagc tcggctactg gacccaggcg ctggccgggc tgcccgaccg
     2281 cctcgatctg cccaccgacc gaccccgccc accggtggcg tcctaccgcg gtgattacct
     2341 gaccatccaa ctagagccga agttgcacca cgggctgacc gacctggccc gccgacacgg
     2401 agccagcctg tttatggtct tgcaggccgg gctggccgcg ctgctgagca aactaggtgc
     2461 cggcaccgac atccccgtgg gcagcccgat cgccgggcgc accgaccaag cactagacca
     2521 gctggtcggg tttttcgtca acaccctggt cctgcgcacc gatgtgtccg gcaaccccaa
     2581 cttcacccaa ctgctgaccc gggtacgcga gaccgccctg tccgcctacg cccaccaaga
     2641 cgtgccgttt gaatacctcg tcgaggtgct taaccccacc cgatccctag cgcatcaccc
     2701 gctgttccaa atcatgctgg cgttgcaaaa cgcccccgag gcccacttcc agctacccgg
     2761 cctcgacgtc aacttcatgc cagcaccaac cggaaccgcc aaattcgact tgggtttgag
     2821 tctgtgggag cggcgcggcc ccgacggcag cccccaaggc atcgacggaa tcgtggaata
     2881 cgccaccgac ctgttcgacc ccaccaccgt ccaaacactg ctaacacgct ggatacgact
     2941 actagacgcg gcagtcaccg accccgacca accaatcagc cgcatcaacc tgctcgacgc
     3001 tcaagaacgc caccagatcc tagtcaaggc caacaacacg gaccatccaa tccctgcggc
     3061 cagcctgcca acgctgttcg aaacccaggt ggcggccaca ccacaggcgg tagccgtggt
     3121 cttcggcgac accaccctca cctaccgcca gctcaacacc aaggccaacc aactcgcgca
     3181 cacactcatc acccaaggag taggtcctga acagatcgtc gcgctagcgc taccccgctc
     3241 acccgagcta gtcgtggcaa tcctggcagt actcaaagcc ggcgctgcct acctacccct
     3301 ggaccccgac taccccacag cgcggatcaa cttcatactc ggtgacgcac agcctgcgct
     3361 gctggtgact agcgaacaga ccgcggggtg tgttccccaa gacgctgcca caccgcagct
     3421 ggtcatcgac caccccgaca ccgtggcggt actgagcggc tgcactgaga ctgatccgac
     3481 cgacattgac cgcaccaccc cccttctgcc gcagcagccg gcgtatgtga tctacacctc
     3541 cggctccacc ggtaccccca agggtgtggt cgtctcccat gttggtgtct ccagtcttgc
     3601 ggcggcgcaa attgaccggc tgggcatagg tgccagcagt cgggtattgc agtttgcctc
     3661 acccagcttc gacgcctcgt tctgggagct gtgtatgggc ctgctgtccg gcgcggcgct
     3721 ggtcttagcc cccaaggatc agctgttgcc cggaacaccg ttgtgcgcac tagtgaaccg
     3781 ccagcaggtg acccatgtga cgttgccgcc ctcggtgttg acggtactgc ccgtcgagga
     3841 cgggttgcca cccgcggtga cggtggtcgt ggccggcgag gtatgcccgg cgggcctagt
     3901 caccgcctgg gcaccaggcc ggcggatgat caacgcctac ggccccaccg agaccacggt
     3961 gtgcgccacc atgagcgacc ccctgccccc cgcgacgcca acaccggtgc cgattggccg
     4021 gccaatcgct aatacccgcg tgtatgtgtt ggatgcgggt ttgcagccga caccggttgg
     4081 ggtggcgggg gagttgtatg tggcgggtgc gggcctggcg cgggggtatc tgcgtcggtc
     4141 tgggttgacc gcggagcggt ttgtggcgga tccgttcggg ccgcccgggg ctcgcatgta
     4201 tcgcacgggg gatctggtct gttggaacgc tgatggggac cttgagtttg tgggccgggt
     4261 ggatgatcag gtcaaggttc ggggtttccg gatcgagccg ggcgagatcg aaacggtgct
     4321 ggccgcccat ccgggtgtgg cccgcagtgc ggtgatcgcg cgggaagacc ggcccgggga
     4381 caagcggctg gtcgcctacg tcgtgcccac cacgcaggac gcgcgccggc cggatgtgct
     4441 gcgcgagtat gtccgccaac gcctacctaa gtatctggtg cccgccgcgt tcgtgatgct
     4501 cgacgggttg ccgttgacac ccaacggcaa gctcgaccgt accgcgctgc ccgcccccga
     4561 cctgaccccg accacgccca gccgtgcgcc ctgttcgctg caggagcaga tcctgtgcga
     4621 gctgctcgcc gaagtgctgg gtctggactg ggtcggtgtc gacgacgatt tcttcgcact
     4681 gggtggggac agcatcatct caattcagct gg
//
LOCUS       DATADN010000891         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_713447, whole genome shotgun sequence.
ACCESSION   DATADN010000891 DATADN010000000
VERSION     DATADN010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713447"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VHH53_11565"
     CDS             <1..1461
                     /locus_tag="VHH53_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX2290802.1"
                     /translation="RVLTSRRRPVVLLPRRPQAAVAPAVAPGNRSLGLMLPYTPLHHL
                     LLRATAAAIVLTSGNISDEPIAYRDDDAQRRLGGIADAFLTHDRAIHMRTDDSVVRVF
                     RGAELPVRRSRGYAPEPLTLAQPVPRPILGCGAELKNTFCLARGRHAFVSHHIGDLEN
                     YETLRSFTDGIAHFGRLFDVAPEVVAHDLHPEYLSTKYAVERADVEPVELIGVQHHHA
                     HIASCLADNSDAGPVLGVAFDGLGYGSDGTLWGGEFLLADLTGFQRLAHLVPVPMPGG
                     ATAIRQPWRMAAAYLGSEVPQELARRNAEQWDAVLAMADHGVNAPLTSSAGRLFDAVA
                     ALLGVRDSINYEGQAAVELEQRADRTEQGSYPAAVTEGPALQLHGADLVRAVVADLQA
                     GVPREVIATRFHHGVADAIVRVCLMLRETTGVSAAALSGGVFQNILLLERTVAGLEQA
                     GFRVLTHSRVPPNDGGISLGQVAVTAALLRSGCRTL"
     gene            complement(1583..>2042)
                     /locus_tag="VHH53_11570"
     CDS             complement(1583..>2042)
                     /locus_tag="VHH53_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290803.1"
                     /translation="IRADAREPKTGKPGAVVPQQVRHQQVGHRRMYARSGTVPGERLH
                     TEGTDVFRRTRIRRDATELAVAGAGLGAEARTATRPGLDLYPNPEPGICARCSYRSPC
                     LVLNHGGDATAELLRSYRPRPAEPVKEGRLGGVTWSMNRGAAPPRWTLNS"
BASE COUNT          301 a          711 c          708 g          322 t
ORIGIN      
        1 cgggtgctaa ccagccggcg gcggccggtg gtgttgctgc cccgccgccc gcaggctgcc
       61 gtcgcaccgg ccgtcgcgcc cggcaaccgg tcgctaggtc tgatgttgcc ctacaccccg
      121 ctgcatcact tgctgcttcg cgccaccgcc gctgccatcg tgttgaccag cggcaacatc
      181 tccgacgagc cgatcgccta ccgggacgac gacgcgcagc gccggctggg cgggatcgcc
      241 gacgccttct tgacccatga ccgggcgatc cacatgcgca ctgacgactc ggtggtgcgg
      301 gtgtttcgcg gtgctgagct gccggtgcgc aggtctcgcg gctacgcccc cgaaccgctg
      361 accctggcgc aaccggtgcc ccggccgatc ctgggctgtg gcgccgagct caagaacacc
      421 ttctgcctgg cccgcgggcg ccacgccttc gtctctcacc acatcggcga tctggagaac
      481 tacgagaccc tgcgctcgtt caccgacggc atcgcacatt ttgggcggct cttcgacgtg
      541 gcgccggaag tggtcgcgca cgacctgcac ccggagtatc tgtccaccaa gtacgccgtg
      601 gagcgcgccg acgtggagcc cgtcgagctc atcggggtgc agcaccacca cgcgcacatc
      661 gcgtcgtgcc ttgccgacaa cagcgacgcc ggtccggtac tgggagtggc ttttgacggg
      721 ttgggttacg gcagcgacgg caccttgtgg ggcggcgagt tcctgctggc cgacctgacc
      781 ggtttccagc ggttggcgca cctggtgccg gtcccgatgc ccggcggcgc caccgccatc
      841 cggcaaccct ggcggatggc cgctgcctac ctgggctcag aggtgccgca ggagctggcg
      901 cggcgcaacg ccgagcagtg ggatgccgtg ctggcgatgg cagaccacgg agtgaacgcg
      961 ccgctgacgt ccagtgcagg acggctgttc gacgccgtgg cagcactcct cggagtccgg
     1021 gactcgatca actacgaagg ccaggccgcg gtggagctcg agcagcgggc tgaccgcacc
     1081 gagcagggca gctacccggc ggcggtgacc gaggggcccg cgctgcagct gcacggcgcc
     1141 gacctggtcc gcgccgtggt ggccgacctg caagccgggg tgcctcggga ggtgatcgcc
     1201 acacggttcc accacggcgt cgccgatgcg atcgtccggg tctgcctgat gctgcgggag
     1261 acgactggag tgagcgccgc tgcgctgtcc ggcggggtgt tccagaacat cctgctgctc
     1321 gaacgtaccg tggccggcct ggaacaggcc ggattccgag tcctcacgca ctcccgcgtg
     1381 ccccccaatg acggcggaat cagcctcggt caagtcgccg tcaccgccgc actactgcgt
     1441 tctggatgca gaacgctgta attcgagccc tccgaaccgc gttctgcatc cagacgcggg
     1501 cacggtcagt cccggaagcc gagcccgctt gcgtggggaa ggtcgtgggt gacgctgcac
     1561 gatatcgagg tccggggcgg tatcaggaat taagagtcca ccggggtggc gccgcaccac
     1621 ggttcatcga ccacgtcaca ccgccgagcc gcccctcctt gaccggctcc gccgggcggg
     1681 gccggtagga ccgcaacagt tcggcggtgg cgtcgccacc gtggttgagc accaggcaag
     1741 gggagcgata ggagcaacgt gcgcagatcc caggctctgg gttgggatag agatcgaggc
     1801 ctggtctggt ggcggtgcgt gcctcagcac ctaggcctgc gccggcgacc gccagctcgg
     1861 tcgcgtcgcg ccggatgcgg gtacggcgaa acacgtcggt gccctcggta tgcagccgct
     1921 cgccagggac agtgccgctg cgggcataca tccgccggtg acccacctgc tgatgccgca
     1981 cttgttgcgg gactacagca cccggcttgc ccgttttcgg ctcgcgcgcg tcagcgcgaa
     2041 tc
//
LOCUS       DATADN010000892         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_251721, whole genome shotgun sequence.
ACCESSION   DATADN010000892 DATADN010000000
VERSION     DATADN010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251721"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..924
                     /locus_tag="VHH53_11575"
     CDS             532..924
                     /locus_tag="VHH53_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290804.1"
                     /translation="MGSSGGPRADRPKRRSFTAAYKAEILEEYERLSDPSDRGALLRR
                     EGLYHSHLQAWRQARERGGLNALSARPAERPSRSKADVENERLRAENERLAGELAKTK
                     AALEVVGKAHALLELLSESADSHGKSDS"
     gene            921..1523
                     /locus_tag="VHH53_11580"
     CDS             921..1523
                     /locus_tag="VHH53_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290805.1"
                     /translation="MIEEAFGELAPLSSTTRACELLGVSRASLYRRRHPPPPAARTPR
                     TPGVHPATLGDDERAQLLRVLNSDRFADRSPTQIWAILLDEGMYLASVSTMYRLLRHH
                     DQTRERRAQATHPAKKKPELLATGPDQVWSWDITKLTGPARGVSYDLYVILDIFSRRS
                     STSTSTPPKPANSPRHSSTPPSPPTAAYDPTRSTPTAAPR"
     gene            1451..>1842
                     /locus_tag="VHH53_11585"
     CDS             1451..>1842
                     /locus_tag="VHH53_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX2290806.1"
                     /translation="MDAAITANGGIRPDAIHADRGTSMTSQPVAALLAALGIDQSHSR
                     PHVSNDNPYSEANFKTLKYCPAFPGNFGSIHDARAFCAIFFTYYNNEHRHSGIGLHTP
                     ATVHNGTAHQIHARRAEVLNAAYTANPQR"
BASE COUNT          333 a          656 c          563 g          290 t
ORIGIN      
        1 cccactgctc ccacaggtgc tccgcgcgat gtgatcgacg cccctggggg acgcgccgat
       61 aaccaggacc gcggtcccgt ctccccagac ctcgaccagg gcgaatggtg gtcgcgttgg
      121 tccaggcagg tctggtgacc aggaccctgc gggtcatcgc ggcacctccg cgggcaccga
      181 ctcgatggag atggtccact cgtggccgca gcgcaggccc acccgggacg gtcgccagtg
      241 ggtcgcccgg tgtcgcttcc gtcaactcaa tggccagacc gtccgcgtcg cgcgctgggg
      301 atcgtccaag actgccgcga cgagcaacct gcaggacgag ttgcgcggcc gcgcaggcga
      361 gcgcccttga tgttgtcggc tgacgcccgt ttcgccgacc cctctctgtc aaactctcgt
      421 gagacatgac gatcatgtga atctgtgtag cgagtagggc gagacaggac tcccaggcca
      481 gtgacgttga ccatggcaga catgccgtcc tccgtgcggg aggatggccg ggtgggctcc
      541 tctggaggac cgcgggcgga ccggccgaag cgccggagct tcactgcggc ctataaggcg
      601 gagattttgg aggaatacga gcggctcagt gatccgagtg atcggggtgc tttgctgcgc
      661 cgggaggggc tgtatcactc gcacctccag gcctggcgcc aagcgcgtga gcggggcggg
      721 ttgaatgcgc tttctgccag gccggcggag cggccttcgc ggagcaaagc tgatgtcgag
      781 aatgagcggc tccgtgcgga aaacgagcgg ctggccggtg agttggccaa gactaaggcc
      841 gcgctggagg tcgtgggaaa agcacacgcg ctcttggaac tgctctccga gagcgcggat
      901 tcgcacggga agtcggattc gtgatcgagg aggcgttcgg cgagctcgcg ccgctgtcgt
      961 ccacgacaag ggcgtgcgag ttgctcggtg tctcacgggc ctcgctgtat cggcggcgtc
     1021 atcccccgcc gccggctgcc cggacgccgc gcacgccggg ggtgcatccg gccacgctgg
     1081 gtgatgacga acgcgcgcag ctgctcagag tgctcaacag cgaccggttc gccgaccgct
     1141 caccgaccca gatctgggcg atcctactgg atgagggcat gtatctggcc agcgtgtcca
     1201 ccatgtaccg gctgctgcgc caccacgacc aaacccgcga gcggcgcgct caggccacgc
     1261 acccggccaa gaagaaaccc gaacttctgg ctaccggacc cgaccaggtc tggagctggg
     1321 acatcaccaa actcaccgga ccggcccgcg gtgtgtccta tgacctgtat gtgatcctcg
     1381 acatcttctc ccgcaggtca tccacttcga cgtccacccc accgaaaccg gcgaactcgc
     1441 ccaggcattc atcgacgccg ccatcaccgc caacggcggc atacgacccg acgcgatcca
     1501 cgccgaccgc ggcacctcga tgacctccca accagtggcc gctctcctcg ccgctttagg
     1561 catcgaccaa tcccattccc gcccacacgt gtccaacgac aacccctaca gcgaggcgaa
     1621 cttcaaaacc ctcaaatact gccctgcgtt ccccggcaat ttcggatcca tccacgatgc
     1681 ccgcgccttc tgcgccatct tcttcaccta ctacaacaac gaacaccgcc attccgggat
     1741 cgggctacac acccccgcca ccgtccacaa cggcaccgcc caccaaatcc acgcccgccg
     1801 tgccgaggtc ctcaacgccg cctacaccgc caacccccag cg
//
LOCUS       DATADN010000893         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1473318, whole genome shotgun sequence.
ACCESSION   DATADN010000893 DATADN010000000
VERSION     DATADN010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473318"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VHH53_11590"
     CDS             <1..628
                     /locus_tag="VHH53_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX2290807.1"
                     /translation="KQLVVIAVLFAAATVFWMAYEKAGSTLNLFADRSTSNSVLGREY
                     PASWYQSLPPLFIILFAPVFAAIWIRLGRRNPSSPAKFALGLFLLGLGFVVMIGAASV
                     AASGVRVSPLWLVVSYLLQTLGELCLSPVGLSAMSTLAPARIAGLVMGVWFLALSVGN
                     YLAGMASSFYESMPLPRLFTIVAATALAAAVVLALLVRPIQRMMTRTS"
     gene            complement(625..1662)
                     /locus_tag="VHH53_11595"
     CDS             complement(625..1662)
                     /locus_tag="VHH53_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX2290808.1"
                     /translation="MPEIGLPDALAAILALSLNAYVLFGGADFGGGVWDLLASGPRRG
                     RQREVIAHAIGPIWEANHVWLILAIVLTFTCFPSVYARLGTVLHIPLTLMLVGIVLRG
                     SAFTFRTYDSQHDATQRRWGRIFASASVITPVLLGVSIGAVASGRVGSALKGTFSDQF
                     IAPWLTPFALSVGLLTLTLFAFLAAVFLTLETKDPDLCEDFRRRALWCGVAVFFASAL
                     VLLLSGEAAPLVRAGLTRSPWALPLHLATGLSAITVLAALWYRRYRLARLAVGLQVSL
                     IFWGWPLAQYPFLLPPDFTIAGTAAPDLTLRLTLIVLAVGGVILLPSLWYLFQVFKTT
                     PADPQRPAAQP"
BASE COUNT          364 a          572 c          593 g          291 t
ORIGIN      
        1 gaagcagctc gtggtaatcg ccgtactctt tgcggccgct accgtattct ggatggctta
       61 cgagaaggcc ggctccaccc tcaacctctt tgctgaccgc agcaccagca acagcgttct
      121 gggacgagaa taccctgcca gctggtatca atcgctgccg ccgctgttca tcatcctctt
      181 tgccccggtg ttcgccgcca tctggatccg gttgggccgg cggaacccct ccagcccggc
      241 gaaattcgcg ctcggtctct ttctgctcgg gctcgggttt gtggtcatga tcggtgccgc
      301 gtcggtggct gcgagcggag tgcgggtgag cccactctgg ttggtagtct cctatctgct
      361 gcagacgctg ggcgagctct gcctcagccc ggtaggactc agcgcaatga gcacgctcgc
      421 accggccagg attgccggcc tcgtcatggg ggtgtggttt ctggctctct cggtgggcaa
      481 ctacctggcg ggcatggcgt cgagctttta cgagtctatg ccgctccccc ggctcttcac
      541 catcgtcgca gccacggcgc tcgccgccgc ggtggtgctg gccctgctgg tgcgcccgat
      601 ccaacggatg atgactcgca cctcctaggg ctgcgctgcc ggacgctgcg ggtcggccgg
      661 cgtggtcttg aacacctgaa agagatacca gagcgagggc aggagaatga ctcctcctac
      721 cgccagcacg atcagggtca ggcggagcgt caagtcaggc gccgccgtgc cggcaatggt
      781 gaagtcaggc ggcaggagaa acgggtactg cgccagcggc catccccaga agatcaagga
      841 gacctgaagt cccaccgcca ggcgcgcgag gcggtagcgc cggtaccaga gtgcggccag
      901 aacagtgatc gcgctgagtc cggtggccag gtgaagtggc agcgcccatg gtgagcgggt
      961 gagtcccgca cgcaccagcg gcgcagcctc cccggagaga agcagaacca gggctgatgc
     1021 aaagaacacg gcgacaccac accaaagcgc ccgccggcga aaatcctcgc acaagtccgg
     1081 atccttggtc tccagggtca ggaacacggc cgccagaaag gcaaacagcg tcagagtaag
     1141 caggcccacg ctgagcgcga atggcgtgag ccacggcgct atgaattgat cgctgaaggt
     1201 tcccttcaag gcgctaccta cccggcccga ggccacagcg ccgatggaga ctcccaggag
     1261 aaccggtgtg atcacgctgg cgctggcgaa gatccgcccc caccgccgct gcgtcgcatc
     1321 atgctggctg tcataggtgc ggaaggtgaa tgcggatcct cgaagcacga ttccaaccag
     1381 catgagggtc agcggaatgt gcagcaccgt gcccagccgg gcgtacaccg acggaaagca
     1441 agtgaacgtc agcacaatgg cgagaatcag ccagacgtga ttggcttccc agattgggcc
     1501 gatggcatgc gcgatcacct cccgctggcg gccgcgccga ggtccggaag caagcaggtc
     1561 ccacacaccc ccgccgaaat cggctccacc gaacaagaca taggcgttca gcgataaagc
     1621 caggatggcg gccagtgcgt ctgggagccc gatctcaggc atggcgggcc ggagaagcgc
     1681 ggtatacgcg gctccagtcg ctcgacttgg gactgcggat gatctggcgg tagagcagcc
     1741 aggccacaat gacggccagg aagcagtaga gcagagtaaa aagcaggaat ggaactacga
     1801 gcccaggcat gggtgtcacc
//
LOCUS       DATADN010000894         3153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_567026, whole genome shotgun sequence.
ACCESSION   DATADN010000894 DATADN010000000
VERSION     DATADN010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567026"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1676)
                     /locus_tag="VHH53_11600"
     CDS             complement(111..1676)
                     /locus_tag="VHH53_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX2290809.1"
                     /translation="MLVVGHGSRDADGVDEFWALAKTIRAVSGDLMTGFGFIELASPT
                     VDEAIDELVACGATEIVSVPLVLLSAGHLKNDGPAALARARTRHPGVHFTLARDLGIE
                     PNVLEVVADRIRDAAGDADPEKLGVALIGRGSSDPDACADLWKVSRLLADGRGLGTVE
                     PGFVSVAMPSVLEALERLRLLGAGIAVVAPFFLFPGVLRDRIYAEAAQWAQDHPDVEV
                     RGAGHLGPDSRLARLVLQRYREALDGDVRMNCDLCAYRVQLPGYESKVGTPISLTPHG
                     GFAAIEVQDLDFSYPDGRQALSGVSMRIEPGERVALLGPNGAGKTSLVLQLNGVLTPS
                     AGSVSIGGLQVGRRTLTEVRRRVGVVFQDPDDQLFSPTVGRDVAFGPAHLGLSGDALA
                     ARVTEALSYVGLADAADRPPHRLSLGERRRAAVATVLAMHPEVLVLDEPTANLDPAAR
                     REFADLIKRLGMTTLLVTHDLPYALELCPRALVIDHGQIVADGPTRELLADTGFMSAH
                     RLELPAGFNPVGN"
     gene            complement(1715..2476)
                     /gene="cbiQ"
                     /locus_tag="VHH53_11605"
     CDS             complement(1715..2476)
                     /gene="cbiQ"
                     /locus_tag="VHH53_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX2290810.1"
                     /translation="MSATHEPGTQLLIAADTPVHRLAPQCKVAATALFVLVVAATPTG
                     TYWPYLWYLLLLSMVAAVAALPPLILLRRLLIEVPFLLFVLALPVLGTSPQVTLLGVD
                     LSVPGLHAAAAIALKATFGLLTTGILAATTPMPEIITGLERLRVPRVFTAVAAFMVRY
                     ADVLLGELARMRTAAACRGGDPRWLWQIRDVSTGLGALVVRSFERGERVYLAMASRGY
                     TGALPTALTGAAASPPAWAAALALPLLAVTGTILA"
     gene            complement(2473..>3153)
                     /locus_tag="VHH53_11610"
     CDS             complement(2473..>3153)
                     /locus_tag="VHH53_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX2290811.1"
                     /translation="PGLMSRPVSPGRAVHIPDGFVSTPVAVAGAAMAVVGLTICVRRA
                     GQDGTERQLPLAGLVAAFFLVVGAPIIPVAVGTSGHLLGGTLAVALLGPWLGPVVITV
                     VTVAQALFLGDGGVSALGVNVVNLALVPAAVGYPLLLGLRRVLPWRPAGLTVAAGVAA
                     VVSVLAAAVLFTVEYALGGAVGVPLHTVAGSVIGVYALVALFEGLVTAVVVRSLLALR
                     ADLVRVAP"
BASE COUNT          569 a         1155 c         1040 g          389 t
ORIGIN      
        1 gacgccacga ccggctcgac gacgtgggcg gacgtcgcgc gcggcgtcgg tggagaccag
       61 cagcgtggtg cgcacgaccc cgttgtgacg gtcacgggcg tctcctcagg tcagttcccc
      121 actgggttga agccagcggg aagctccagc cggtgagcgc tcatgaatcc ggtgtcggcg
      181 agcagttccc gggtgggccc gtcggcgacg atctgcccgt ggtcgatcac cagcgcgcgc
      241 gggcacagct ccaacgcata gggcagatca tgggtgacca gcagcgtcgt catcccgagc
      301 cgcttgatca aatcagcgaa ctcgcgccgg gccgccgggt cgaggttggc ggtgggctcg
      361 tcaagcacca gcacttcggg atgcatggcc agcaccgtgg cgaccgcggc gcgtcgccgc
      421 tcacccagcg acagccggtg cggtggccgg tcggcagcat cggccagacc gacataggac
      481 agcgcctcgg tgacccgggc cgctaaagca tccccggaca atcccagatg cgcgggtcca
      541 aaggcgacat cccgaccgac ggtggggctg aacagctggt catccgggtc ctgaaagacc
      601 acgccgactc gcctgcggac ttcggtcagg gttcgccgcc ccacctgcag gccgccaatg
      661 gataccgagc ccgccgacgg cgtgagcacc ccgttgagct gcaacaccag cgacgtcttg
      721 cccgcaccgt tgggaccgag cagggcgacc cgctccccgg gctcgatgcg catcgacacc
      781 ccggagagtg cttgccggcc gtccggatag ctgaaatcaa ggtcttgcac ctcgatcgcc
      841 gcaaagccgc catgcggggt cagcgagatt ggcgtaccga ccttggactc gtatccgggc
      901 aactggaccc ggtaagcaca caggtcgcag ttcatccgca cgtcgccgtc gagcgcctcc
      961 cggtagcgct gcagtaccag ccgggccagc cgcgagtcgg gtcccaggtg accggcgcca
     1021 cggacctcga cgtcggggtg gtcctgagcc cattgggcgg cctcggcgta gatccggtcc
     1081 cgcagcacgc cggggaacag gaagaacggt gcgaccacgg cgatccccgc gccgagcagc
     1141 cgcaaccgtt ccagagcctc tagtaccgac ggcatcgcga ccgagacgaa cccgggctcg
     1201 acggtgccca gcccccggcc gtcggcgagc aaccggctga ccttccagag gtccgcacag
     1261 gcgtccggat cgctggaccc acggccgatc agcgccaccc ccagcttctc cgggtcggcg
     1321 tcaccggccg cgtcccggat ccggtcggcg accacctcca ggacgttcgg ctcgatgcca
     1381 aggtcgcggg ccaaggtgaa gtgcactcct gggtgccggg tccgggcccg agccagtgcg
     1441 gcgggaccgt cgtttttcag gtgcccggcg gacagcagca ccagcggcac gctgacgatc
     1501 tccgtggcgc cgcaggccac cagctcgtcg atggcctcat cgacggtagg cgacgccagc
     1561 tcgatgaaac caaaaccggt catcaggtca ccactgacgg cgcggatggt cttggccagc
     1621 gcccagaact cgtccacccc gtcggcatcg cggctgccgt gccccacaac cagcatcgct
     1681 ggcctgatgg ctcgaccctg cgagcaggtc tcgctcacgc gaggatcgtc cctgtcaccg
     1741 ccagcagcgg cagtgcaagc gctgccgccc atgccggagg agacgccgca gcaccggtca
     1801 gcgcggtcgg tagtgccccg gtgtaaccgc gagacgccat cgcgagatag acccgttcgc
     1861 cacgctcgaa gctgcggacc accagcgcgc cgagtccagt cgacacgtcg cggatctgcc
     1921 acagccagcg cggatcgccg ccccggcagg ccgcagcggt gcgcatcctg gccagctcac
     1981 cgagcagcac gtcggcgtag cgcaccatga atgccgcgac cgcggtgaac acccgaggca
     2041 cccgtagccg ctccagcccg gtgatgatct ccggcattgg cgtggtcgcg gccaggatcc
     2101 cggtggtgag cagcccgaac gtcgccttca gtgcgatagc tgccgcggcg tgcagcccag
     2161 gcaccgacag gtccaccccg agcagcgtca cctgtgggga tgtgcccaga actggcagag
     2221 ctagcacgaa cagcaggaac ggcacctcga tcagcaaccg ccgcagcagg atcagcggcg
     2281 gcagggcagc caccgcggcg accatggaca gcagcagcag ataccacaag taaggccagt
     2341 acgtgccggt tggagtggct gcgacgacca gcacgaacag cgcggtggcg gcgaccttgc
     2401 actgcggtgc cagccggtgc accggggtgt cggcggcgat gagcagctgc gtgcccggct
     2461 cgtgcgtcgc actcatggag ccacccgtac caggtcagcg cgcagcgcca gcagggagcg
     2521 caccaccacg gcggtgacca gcccctcgaa cagcgccacc agcgcgtaaa ccccgatcac
     2581 cgacccggcg acggtgtgca gcggcactcc caccgcacca cctagcgcgt attccaccgt
     2641 gaacagcact gcagcggcga gcaccgacac caccgcggcg acaccggcgg ccaccgtgag
     2701 tccggcgggt cgccagggca gcacccggcg cagcccaagc agcagcgggt agcccactgc
     2761 ggccggcacc agggccagat tcaccacgtt gacaccgagt gcggacaccc cgccatcgcc
     2821 cagaaacagc gcctgggcca ccgtgaccac agtgatcacc accggtccca gccatggtcc
     2881 gagcaacgcc acggccaggg tgcccccgag cagatgccca ctggtgccca ccgcgaccgg
     2941 aatgatcggc gctcccacga ccaggaagaa cgccgcgacc aacccggcta gcggtagctg
     3001 ccgctcggta ccgtcttgtc ctgcgcgccg tacgcagatc gtcagcccga ccacagccat
     3061 tgcggcaccg gcgaccgcga cgggagtgga cacgaacccg tcagggatgt gcacggctcg
     3121 tcccggtgac accggccgtg acatcaaacc ggg
//
LOCUS       DATADN010000895         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1473435, whole genome shotgun sequence.
ACCESSION   DATADN010000895 DATADN010000000
VERSION     DATADN010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473435"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VHH53_11615"
     CDS             complement(<1..476)
                     /locus_tag="VHH53_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290812.1"
                     /translation="MSETIGQPRETAALPRPLGIGEILSTAFQLYRRHWRTLLTIAAV
                     VVVPITLLHYLVGDLVRTQGETTRNGVVETATWSVGIAGLVAALAGILMYLVLTGAIT
                     RAVAAEVAGQDPSVEQSYRFGFHRLGPVLLVSVLVGLATIGGFILLVIPGIYIGVRL"
     gene            complement(734..1687)
                     /locus_tag="VHH53_11620"
     CDS             complement(734..1687)
                     /locus_tag="VHH53_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX2290813.1"
                     /translation="MKAFIYEKYGPPETLRMVEVDKPAPNPDHVLVNVLAVSVNAADW
                     HVLRGKPLFSRATLGLLRPKHRILGVDVAGQVEAVGSDGTGFKPGDEVYANLLDHGYG
                     GFAEYVSVPVEAMSAKPASLSFEEAAAVPMAATTALQGLGRHGELQPAQKVLINGGSG
                     GVGTFAVQIAKAYGAEVTAVTSARNLDLVRSLGADQVIDYAKEDFTRTGRRWDRILDT
                     VGNRSAPELRRALAEGGKAAVTGFTSVRRLLGVSLRGGKDIAQVQAHVTTRDLALLSE
                     LIEAGKLRPRIDRRYRFADIPAAIAYQEQGHARAKVVVAMT"
     gene            complement(1946..>2180)
                     /locus_tag="VHH53_11625"
     CDS             complement(1946..>2180)
                     /locus_tag="VHH53_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX2290814.1"
                     /translation="VPRGMAMLGLIGGPLVIASGVAIVLGVIEAGSVWQVIATAPEFV
                     WELSLGIYLMAKGFRPSPILPEGSAAAGRYAES"
BASE COUNT          330 a          738 c          750 g          362 t
ORIGIN      
        1 agccgcaccc cgatgtagat gcccgggatg accagcagga tgaacccgcc gatggtcgcc
       61 agcccgacca gcacgctcac cagcaggacc ggccccagcc ggtggaagcc gaagcggtag
      121 ctctgctcca cgctcgggtc ctgaccggcc acctcggcag ccaccgcccg agtgatggcc
      181 ccggtgagga ccaggtacat caggatgccg gcgagcgcgg ccaccaggcc cgcgattccc
      241 accgaccagg ttgccgtctc cacaaccccg ttgcgagtag tctcgccctg ggtgcggacc
      301 aggtcgccga ccaggtagtg cagcaaggtg atgggaacga cgaccacggc ggcgatggtc
      361 agcagggtgc gccagtgccg tcggtagagt tggaatgcgg tgctcaggat ctcgccgatg
      421 cccagcgggc ggggcagggc tgctgtctct ctaggttgtc cgatggtctc gctcatgtcc
      481 tgacctccag ctggtgttcc tgcgtcggtg gtatgcgttg gtggcggccg ctgggtggag
      541 gcggctggtg gctcagggca gctgcgccgg tcgatctgat gctcctgacc ggtacgccgt
      601 gcagccgttc cggtcctcga cgcctgatgg ccatcgaagg cggctccatg tgtgttgctc
      661 cttcacgtgg gggtgggtca tgaggccagt cggtggtgtt ggcgctgcac ggcgttgacg
      721 atcctggtcg gcctcatgtc atcgccacga ccaccttggc cctggcgtgg ccctgctcct
      781 ggtaggcgat cgcggcgggg atgtcggcga accggtagcg ccggtcgatc cgcgggcgca
      841 gcttgccggc ctcgatcagc tccgagagca acgccagatc cctggtggtg acgtgggcct
      901 gcacctgggc gatgtccttg ccgccgcgca gcgacacccc cagcagcctc cgcacgctgg
      961 tgaagccggt gacggcggcc ttgccgccct cggccagcgc ccgccggagc tcgggcgccg
     1021 accggttccc gaccgtgtcg aggatgcggt cccagcgccg cccagtacga gtgaagtcct
     1081 ctttcgcgta gtcgatgacc tggtcagcgc cgagggagcg gaccaggtcg aggttgcggg
     1141 cgctggtcac ggcggtcacc tcggccccgt aggccttggc gatctggacc gcgaaggtcc
     1201 cgacaccgcc agagccgccg ttgatcagga ccttctgggc aggctggagc tctccgtggc
     1261 ggccaagacc ctggagggcg gttgtcgccg ccatgggaac ggcggccgcc tcctcgaacg
     1321 acaggctcgc cggcttggcc gacatggcct ccacgggcac cgagacgtac tcggcgaagc
     1381 cgccgtagcc gtggtccagg aggttggcgt agacctcgtc gccgggcttg aacccggtgc
     1441 cgtcgctgcc gaccgcctcg acctggccgg caacatcgac gccgaggatc ctgtgcttgg
     1501 gtcgcagcaa ccccagggtg gcccgggaga acagaggctt gccccgcagg acatgccagt
     1561 ccgcggcatt gacggatacc gccagcacat tcacaagcac gtgatcgggg ttcggggcag
     1621 gcttgtcgac ctcgaccatc cggagcgtct cgggtggccc gtacttctcg tagatgaacg
     1681 ccttcatgac tggcccttcc ggttccgtag cgtcgccaag cctagtcgac cgttgcgatc
     1741 ggggaattga ctgaggcgag agtctccctg cggcgcctcc tctacgggct cggcgagcgg
     1801 ttggaaggcc tctttgcgac cgaggcgctt agctggcgga ggactcgtcg cttgtaggcc
     1861 cccagttgcg cgcctgagga cgaggaagcg cggcccagct gctcgtaagg cgcgtttcgc
     1921 ctgccgttcg cgcgccacgc ggttctcaag attcagcgta ccgtcccgcc gccgcggacc
     1981 cttctggcag gattggagac ggcctgaacc ccttggccat gaggtagata ccgagcgaca
     2041 gctcccacac gaactctggg gcggtcgcga tgacctgcca cacagagcct gcctcgatga
     2101 cccctaacac gatggcgacc cccgaagcga tgaccagggg accgccgatg agccccagca
     2161 ttgccatgcc ccgtggcacc
//
LOCUS       DATADN010000896         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1304987, whole genome shotgun sequence.
ACCESSION   DATADN010000896 DATADN010000000
VERSION     DATADN010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304987"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..871)
                     /locus_tag="VHH53_11630"
     CDS             complement(146..871)
                     /locus_tag="VHH53_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX2290815.1"
                     /translation="MAEEQGQVQRVLMVAAHPDDLDFGSAGTIATWTDAGLEVSYCIV
                     TDGDAGGFDPQVPRSVIGSIRQDEQRKAAATLGVADVEFLGYPDGRLTVSFELRRDIA
                     RAIRRVRPDRVVVPFPQRDLRNLYVSHPDHQAAGEAALCAVYPDARNPFAHPELLADE
                     GLEAHTVPEVWVASLNDRADHYVDITDTFDRKIAALRAHVSQTAHMTALEERMRAWGS
                     MQARAAGLAEGRLAEGYLVLDTR"
     gene            1058..1498
                     /locus_tag="VHH53_11635"
     CDS             1058..1498
                     /locus_tag="VHH53_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2290816.1"
                     /translation="MAGTDGQSWKVGQLARLAGLTVRTLHHYDQVGLLSPSARTPSGH
                     RLYGEPDVHRLYRIVALRELGLSLEVIGDLLTGEPDLPDLLRQHLAHGGMAAAHRAGA
                     GRTGRRHRPWRTGGAGTGAALDGTAGVLPRRRPRPSRLAVSHAG"
BASE COUNT          262 a          527 c          549 g          258 t
ORIGIN      
        1 cgagacgcgg ccctacccgc agccgccctc ggctaacgcg gtcactgccc cctagctccc
       61 gagctcaaca gcagtgctgt cggtgcgact tttggcagct ctgctgttga gttcgggacg
      121 gggaggacgc tgagagcaac ccgggtcagc gggtgtcaag cacgaggtag ccctccgcca
      181 gccggccctc cgccagccca gcggcccggg cctgcatgga accccaggcg cgcatccgct
      241 cctccagggc cgtcatatgt gcggtctggc tgacgtgcgc gcgcagcgcg gcgatcttgc
      301 ggtcaaaggt gtcagtgatg tcgacgtaat ggtcggctcg gtcattgagg ctggccaccc
      361 acacttccgg gacggtgtgt gcttccagcc cctcgtcggc gagcagttcg ggatgcgcga
      421 acggattgcg ggcgtcgggg tagacggcgc acagtgcggc ttccccggca gcctggtggt
      481 cgggatgact cacgtagaga ttccgcaagt cgcgttgcgg aaacgggacc accactcggt
      541 ccggacgcac tctacggatg gcgcgcgcaa tgtcccgccg cagttcgaag ctcaccgtga
      601 gccggccgtc ggggtaacca aggaactcaa cgtcggcgac acccagcgtg gcggcggcct
      661 tgcgctgctc gtcctgccgg atgctgccga tcaccgagcg ggggacctgc ggatcgaaac
      721 caccggcgtc cccgtcggtc acgatgcagt aggacacctc aagaccagcg tcagtccagg
      781 tggcgatcgt gccggcggat ccgaagtcca ggtcgtcggg atgtgccgcc accatcagga
      841 ctcgctgtac ctgcccctgc tcttctgcca cggcacgctc ctacgctgga gacgcccaat
      901 gtgagcgtca ctgacaggca acgagaatca tcccggcagc cttcccgggg cccgagttgc
      961 ggtggaggcg ccaggacttg aacctgacgt agcggcagga cttagcgtcc gttgtgctac
     1021 cgcgaccggc gggggcacga ggagggatga ggacgcgatg gccggcaccg acgggcagtc
     1081 ctggaaggtg gggcagcttg cccggctggc cggattgacc gtgcggacgc tgcaccacta
     1141 cgaccaggtc gggttgctgt cgccgtcggc gcggacaccg tcggggcacc ggttgtacgg
     1201 cgaacccgac gtgcaccgcc tttaccggat cgtggcactg cgcgagctgg gactgtccct
     1261 ggaggtgatc ggcgacctgc tgaccggtga gccggacctt cctgatctgt tgcgacaaca
     1321 cctggcgcat ggcggaatgg ccgcagctca tcgcgcaggt gcaggccgaa ctggacgccg
     1381 gcaccgaccc tggcgcaccg gcggtgcagg cactggcgcg gcgctggatg gaactgctgg
     1441 agttcttcca cggcggcgac cccggccttc gcgactcgct gtatcgcatg caggctgaca
     1501 acagcgagat gctccagcag caatacggcg ggccgtcacc ggaatgatcg actacatccg
     1561 tcgggcgaat gccgtgtcat ccacctgacg cggttt
//
LOCUS       DATADN010000897         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1305013, whole genome shotgun sequence.
ACCESSION   DATADN010000897 DATADN010000000
VERSION     DATADN010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305013"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VHH53_11640"
     CDS             complement(<1..311)
                     /locus_tag="VHH53_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX2290817.1"
                     /translation="MTGFSTRAIHAGQDPDPHTGSVIVPIHATSTFAQDGVGGLREGY
                     EYARTGNPTRTALETCLASLEGGRYGRAFASGMAATDALLRATCQPGDHLIIPDDAYG
                     GT"
     gene            complement(321..959)
                     /locus_tag="VHH53_11645"
     CDS             complement(321..959)
                     /locus_tag="VHH53_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX2290818.1"
                     /translation="MAPPPQFGRPPPAVRQQADPQARYSLAINGAQPQLDPPDLDIPQ
                     QQWAGTPSYGQPDRDVAGVAAVPGPLADFGTRAVSSLIDYVIPVIALNVLFTIGVATG
                     TVALSPMLTVVGYLGLFTFIGWNSCYRQGTTGQSIGRQGAGTKLVEMKTGQPIGFGRA
                     VLRQLCHSLEFFVGYLWPLWDANHQTFADKIAGTVVIRIEGTTEDRSDNSSP"
     gene            complement(1270..>2571)
                     /locus_tag="VHH53_11650"
     CDS             complement(1270..>2571)
                     /locus_tag="VHH53_11650"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX2290819.1"
                     /translation="GHVAPLVLAKVEYLNPGGSVKDRIALRMVEAAERSGVLQPGGTI
                     VEPTSGNTGVGLALIAQRKGYRCLFVCPDKVGQDKRDVLKAYGAEVVVCPTAVAADHP
                     DSYYSVSDRLAEEIDGAWKPDQYSNPENPASHYHSTGPELWAQTEARITHFVAGIGTG
                     GTISGTGRYLKEVSNGRVRVVGVDPEGSVYSGGTGRPYLVEGVGEDFWPRTYDRTVCD
                     EIVPVSDTDSFAMTRRLAREEGLLLGGSGGMAVVGALRVAQRAAPDSVVVVLLPDGGR
                     GYLAKVFNDAWMASHGFLPPDSYGATVTDVLVGKDGALPKLVHTHPNETVAEAVMILR
                     EFGVSQLPVVATEPPVMAAEVIGAVNERTLLQALFAGKAQLADRIEGHMAAPLPTIGG
                     GESIGSLMQALADADGAMVLIDGKPAGVVTRQDVLGFLAGR"
BASE COUNT          420 a          853 c          847 g          451 t
ORIGIN      
        1 gtgccgccgt aggcgtcgtc ggggatgatg aggtggtcgc cgggctggca ggtcgcgcgc
       61 agcagggcgt cggttgctgc cattccggac gcgaatgctc gcccgtaccg gccgccctcc
      121 agtgaggcga gacaggtttc cagcgcggta cgggtgggat taccggtgcg ggcatattcg
      181 tatccctcgc gcagcccgcc cacgccatct tgggcgaagg tggacgtggc gtggatgggc
      241 acgatcactg atccggtgtg cggatccgga tcctgcccgg catggatggc gcgagtggag
      301 aagcctgtca cctcgccagc ctacggtgag ctgttgtcgc tgcgatcttc ggttgtaccc
      361 tcgattcgga tcaccactgt gccggcgatc ttgtctgcga aagtttggtg attggcatcc
      421 cacagtggcc acaggtaacc gacgaagaac tccaggctgt ggcagagctg acgcaacact
      481 gccctgccga atccgatcgg ctgaccagtc ttcatctcca ccagcttcgt tcctgcccct
      541 tggcgaccga tgctttgccc cgtggtgccc tgccggtagc aggagttcca gccaatgaaa
      601 gtgaacaatc cgagataacc cacgacagtg agcatcgggc tcaatgccac ggtcccggtg
      661 gcgacaccga tggtgaacag gacgttcaag gcgatgaccg ggatcacgta gtcgatcagg
      721 gagctgaccg ccctggtccc gaagtccgcc agcggcccgg gcaccgcagc gactcccgcc
      781 acatcgcgat cgggctgccc gtaggacggt gtaccggccc attgctgctg cgggatgtcg
      841 aggtccggag ggtcgagttg cggttgtgca ccgttgatcg ccaagctgta gcgggcctgc
      901 gggtcggcct gctgccggac ggcgggggga ggtcgtccga attgtggtgg gggcgccaac
      961 cgtggtaatc gcccgaactg aggtgggggc gcaagctcct ggggacagct gatgagtcgc
     1021 cggctttgct ggtcgggtgg ttgtagcacc gggatcgggc cggccgggtg gagctgcgtc
     1081 ccggtcgatc tgaactctgg tcgcgtatcg gttggctcca cttgcccgtt cgtcccggac
     1141 cgaacttgct cgtctgcgcg gggatcctga tgcagcggct ggggagtggt catcgggttc
     1201 gaactccctc cgatcggtgg ccgggcacgt tcgtgcgcgg tcacgctagc ggatccctcc
     1261 agcccgccct cagcgcccgg ccaggaaccc gagcacgtct tgccgggtga ccaccccagc
     1321 cggcttgccg tcgataagga ccatggctcc gtcggcgtca gccaatgcct gcatcaatga
     1381 tccgatcgac tcgccaccac cgatcgtcgg caacggcgca gccatgtggc cctcgatccg
     1441 gtcggcgagt tgcgccttac cggcgaacag ggcctgcagc agggtgcgct cattgacggc
     1501 gccaatcacc tcggccgcca tcaccggtgg ctcggtggcg accacgggca gctgagagac
     1561 cccgaactca cgcaggatca tcacagcctc ggcgaccgtc tcgttcggat gggtgtgcac
     1621 cagcttcggc aaggcaccgt ccttgccgac cagcacatcg gtgacggtcg ctccataaga
     1681 atccgggggc aagaagccgt gggaggccat ccaggcatcg ttgaagacct tggcgagata
     1741 tccgcgacca ccgtcgggaa gcaacaccac gaccactgag tcgggggcgg cccgttgcgc
     1801 cacccgtagc gcgccgacga cggccatccc gccggatccg cccagcagca gcccttcctc
     1861 ccgggccagc cgccgggtca tggcgaacga gtcggtgtcc gagaccggga cgatctcatc
     1921 gcagaccgtc cggtcgtacg tacgaggcca gaagtcctcc ccgacccctt caaccaggta
     1981 gggccggccg gtgccgcccg agtacaccga gccctccgga tcgacgccga cgactcgtac
     2041 ccggccgttg ctcacctcct tgagatagcg gccggtgccg gagatcgtcc cgccggtgcc
     2101 gatgccagcc acaaaatgcg tgatccgcgc ctcggtctgg gcccacagtt ccgggccggt
     2161 gctgtggtaa tggctggccg gattctccgg attggagtac tggtctggct tccatgcccc
     2221 gtcgatctcc tcggccagcc ggtcggacac cgagtagtac gagtcggggt ggtcagcggc
     2281 gaccgcggta gggcatacca cgacctcagc gccgtaggcc ttgagtacgt cgcgcttgtc
     2341 ctgaccgacc ttgtccgggc agacaaaaag gcagcggtac cccttacgct gagcaatcag
     2401 cgccaggccg acgccggtgt ttcccgaagt cggctccacg atggtgcccc caggctgcag
     2461 tacaccggat cgctctgccg cttccaccat ccgaagcgcg atccgatctt tcacgctgcc
     2521 gcccgggttg aggtattcca ccttcgccag taccagtggt gcaacgtgcc c
//
LOCUS       DATADN010000898         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_95353, whole genome shotgun sequence.
ACCESSION   DATADN010000898 DATADN010000000
VERSION     DATADN010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95353"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VHH53_11655"
     CDS             complement(<1..996)
                     /locus_tag="VHH53_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2290820.1"
                     /translation="MRLRRTTGVGPKPSAPQLIGIDDLKTPSWAQVMETVAGTGIGRT
                     LRAAPTALRVLVRLAWQTSPRLTLLAAAVELVAGCAMAFGLLATANVLTELLAQGPTP
                     QRVVAALPALVLVMASFAARGLLDAAVGTVQAVLAPRVELRAQNELHAAVIGVELIAF
                     DDPDFVELVRRTSAQGIRSVRYGVGDVGNLLSSTVSLTAAIVTAGLLNPVLAPVVLLA
                     AVPNGWASVRAALLDYDSFVRTISRMRRLGITGDIITGRREAPEVRAFTTQDTLLGEH
                     RRIGSQLTAEAVRVEHRKTAIRLFGRGLAGIGTALAYGVLGLLLYAGAMPLALAGA"
     gene            1046..>2010
                     /locus_tag="VHH53_11660"
     CDS             1046..>2010
                     /locus_tag="VHH53_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX2290821.1"
                     /translation="MSNGDADVRAGIVVTGTELLTGQLTDRNGPWLAQQLGARGVEVA
                     HLLCVGDRAQDLAAALRFLAEEGVDLIVTSGGLGPTADDLTAEVVAGFAGVPLELDQA
                     MEQQIAAILARFRHLSHFSPEALQAANRKQAMVPRGAEPLDPIGTAPGLVISVPSGPT
                     VIVLPGPPRELQGMWPAALSTSAARAVLARARPFESARLRLFGLPESEIAATLREVGA
                     TLDLAPLEITTCLRRAELEIDIRHRPGAEAVRDALIETIRSRHSQYLFSTDGTSLDDQ
                     LADLLQGRRIGLGESCTGGLLAARLTERPGSSNYVAGGVVAYTNTA"
BASE COUNT          314 a          734 c          675 g          287 t
ORIGIN      
        1 cgccccggcc aacgccagcg gcatcgcccc tgcgtagagc agtagaccga gcacgccgta
       61 ggccaacgcc gtgccgatcc cggccagccc ccgcccgaac agccggatcg cggtcttgcg
      121 gtgctccact ctgacggctt ccgcggtgag ctgggatcca atccggcggt gctcgcccag
      181 gagggtgtcc tgggtggtga aggcgcgaac ctcgggtgcc tcgcgccgcc cagtgatgat
      241 gtcgccggtg attcccagcc tgcgcatgcg cgagatcgtc cgcacgaatg agtcgtagtc
      301 cagcagcgct gcccgcacgc tggcccagcc gttgggcacc gcggccagca gcaccaccgg
      361 cgccagcacg gggttgagca ggcctgccgt gacgatcgcg gcggtcagcg aaaccgtgga
      421 gctgagcagg ttcccgacat cgcccacccc gtaacgcacg ctcctgatgc cctgcgccga
      481 cgtccggcgc accagctcca cgaaatccgg gtcgtcgaag gcaatcaact ccacgccgat
      541 caccgcggcg tgcagctcat tctgggcgcg cagctcgact cggggcgcca gcaccgcctg
      601 cacggtgccc accgccgcgt ccagcaggcc cctggcggcg aaggaggcca tcaccagcac
      661 cagcgccggt aatgccgcga ccactcgctg cggagtcggg ccctgtgcca gcagctcggt
      721 caacacgttc gcggtggcca gcaacccgaa cgccatcgcg catccggcga ccagctcgac
      781 agccgcggcg agcaacgtca accgtggcga tgtctgccac gccaggcgca ccaacacccg
      841 cagtgccgtc ggcgccgccc gcaacgtccg gccgataccc gtgccggcca ctgtttccat
      901 cacctgcgcc caggacgggg tcttgagatc atcaatcccg attagctgcg gcgctgatgg
      961 ctttgggcca acaccagtgg tccgcctgag tctcatcgtg gcctcccacc gacaaagcat
     1021 gcctcccgcg ctagcgtgcc gtctcgtgag taacggcgac gcagacgtcc gcgcggggat
     1081 cgtggtcacc gggacggagc tgctcaccgg gcagctcacc gaccgcaacg gcccatggct
     1141 ggcgcagcag ctgggcgcgc ggggcgtcga ggtggcgcac ctgctgtgcg tcggcgaccg
     1201 ggcacaggac ctggcagcgg cacttcgatt cctggccgag gagggcgtgg atttgatcgt
     1261 caccagcggt gggctcggcc cgaccgccga tgacctgacc gccgaggtgg tcgccgggtt
     1321 cgccggggtg ccgctcgagc tcgaccaggc gatggagcag cagatcgccg ccatcctggc
     1381 ccgattccgc cacctgtcgc acttcagccc ggaagcgctg caggcagcaa accgcaagca
     1441 ggcgatggtg ccccgcggcg ccgaaccgct cgacccgatt ggaactgcgc ccgggctggt
     1501 catctcggtg ccttcgggtc cgacggtaat cgtgctgccc ggcccgcccc gcgagttgca
     1561 ggggatgtgg cccgcggcac tgtccacctc cgccgccagg gcggtgctgg cgcgtgcccg
     1621 acccttcgag tcagcgcggc tgcggctgtt cgggctgcct gagtcggaga tcgcggcgac
     1681 gctgcgcgag gtgggcgcga cgctggacct ggccccactg gagatcacca cctgcctgcg
     1741 ccgagccgaa ctggaaatcg acatccggca ccggccgggc gccgaggcgg tccgggatgc
     1801 gctgatcgag acgatccgca gccgacattc tcagtacctg ttctcgaccg acggcacctc
     1861 cctggacgac caactggccg atctgctgca aggacggcgc attgggctcg gggagtcgtg
     1921 caccggtggg ttgctggccg ctcgactcac cgagcggcca ggatcctcga actacgttgc
     1981 aggcggtgtg gtggcctaca ccaataccgc
//
LOCUS       DATADN010000899         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1305053, whole genome shotgun sequence.
ACCESSION   DATADN010000899 DATADN010000000
VERSION     DATADN010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305053"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /locus_tag="VHH53_11665"
     CDS             complement(<1..2045)
                     /locus_tag="VHH53_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX2290822.1"
                     /translation="MALNRPVTASSTEHAGTPASAAVDGNTGTRWSSAFTDPQWIRVD
                     LGSAQTICRVTLTWETAYARAFQIQVSGNGTDWTTIFSTTTATGGTQQLSVTGSGRYV
                     RMNGTVRATGWGYSLWELSVNTDDGSNPGDPVPPTDPRNPNLGPNTFVYSPATPASEI
                     QSRLNSIATQMRTNQFGTQRYAVLFRPGSYNADVNLRFYTQVAGLGLSPDDVNINGHV
                     RVEADWLQQGSDPNNLGNATQNFWRAAENLSVTLPAGQIERWAVSQAAPYRRMHLRGQ
                     VQLWNGGDGWASGGLIADSRIDGLVQSGSQQQFLTRNSQLNNGWSGSVWNMVFVGTAG
                     APPQNFPNPSHTVVASTPKVREKPFLYVDGSGSYNVFVPAIRTNTQGTSWGAGAPAGT
                     SISLSQFYVVHPSSPVSTINAALSQGKHLLFTPGVYRLTDTIRITRPDTVVLGLGLAT
                     LIPETGLPAVTVSDVDGVKVAGLLIDAGSVNSPVLMEVGPPGSSASHAANPTSLHDVF
                     FRVGGAHVGRATVSLRINSHDVIGDHTWIWRGDHGTGVGWTVNTGANGLVVNGNNVTM
                     YGLFVEHYQQYNTIWNGNGGRTYFYQNEMPYDPPNQAAWMNGSTRGYAAYKVADSVTT
                     HEAWGLGSYCYFNVDPSVVAERSFEVPRVAGVRFHNLVTVSLGGVGTINRVINDTGGT
                     "
BASE COUNT          315 a          753 c          815 g          380 t
ORIGIN      
        1 gtgccgccgg tgtcgttgat gacccggttg atggtgccga ccccgccgag cgagaccgtc
       61 accaggttgt ggaaccgcac gccggcgacc cgcggcacct cgaaggaccg ctcggccacc
      121 accgacgggt caacgttgaa gtagcagtag ctgcccaggc cccaggcctc gtgcgtggtg
      181 accgagtcgg ccaccttgta ggcggcgtac ccgcgggtgg acccgttcat ccacgccgcc
      241 tggttgggcg ggtcgtacgg catctcgttc tggtagaagt acgtccgccc gccgttgccg
      301 ttccagatcg tgttgtactg ctggtagtgc tcgacgaaca ggccatacat cgtcacgttg
      361 ttgccgttga cgaccaggcc gttggcgccg gtgttcaccg tccagcccac gccggtcccg
      421 tggtcgccgc gccagatcca cgtgtgatca ccgatcacgt catggctgtt gatccgcagg
      481 ctcaccgtgg cccggccgac atgcgcgccg ccgacccgga agaacacgtc gtgcagcgac
      541 gtcgggttcg ccgcgtggct ggccgaggat ccgggcgggc cgacctccat cagcaccggc
      601 gagttcaccg atccggcgtc gatcagcagg ccggccacct tcacaccgtc cacatcggac
      661 acggtgacgg ccggcaggcc ggtctccggg atcagcgtgg ccagccccag gccgagcacg
      721 acggtgtccg gcctggtgat ccgaatggtg tcggtgagcc ggtagacccc cggggtgaac
      781 agcaggtgtt tgccttggct gagcgccgcg ttgatggtcg acaccggcga actcgggtgc
      841 accacgtaga actggctcag cgagatcgag gtgccggccg gagcgcccgc gccccacgag
      901 gttccctgcg tgttcgtccg gattgccggt acgaacacgt tgtaggaacc ggagccgtcg
      961 acgtacagga acggcttctc gcgcaccttc ggggtactgg ccacgacggt gtgcgacggg
     1021 ttcgggaagt tctgcggcgg cgcgccggcg gtgccgacga acaccatgtt ccacaccgaa
     1081 cccgaccagc cgttgttgag ctggctgttg cgggtgagga actgctgctg cgagcccgac
     1141 tgcaccaggc cgtcgatgcg ggagtcggcg atcaggccgc cgctggccca gccgtcgcca
     1201 ccgttccaca gctgaacctg accacgcagg tgcatccggc ggtacggcgc cgcctgcgac
     1261 accgcccacc gctcgatctg gccggccggc agggtcaccg acaggttctc ggccgcgcgc
     1321 cagaagttct gcgtggcgtt gcccaggttg ttcggatcgc tgccctgctg cagccagtcg
     1381 gcctcgaccc ggacgtgccc gttgatgttc acgtcgtccg gactcagccc gaggccggcc
     1441 acctgcgtgt agaaccgcag gttcacgtcg gcgttgtagc tgccgggccg gaacagcacg
     1501 gcgtagcgct gggtgccgaa ctggttggtg cgcatctggg tggcgatcga gttcagccgg
     1561 ctctggattt cgctggccgg ggtggccggg ctgtagacga acgtgttcgg cccgaggttc
     1621 gggttgcgcg gatcggtcgg cggcaccggg tcacccgggt tgctgccgtc gtcggtgttc
     1681 accgacagct cccagagcga gtagccccac cccgtggccc ggacggtgcc gttcatccgc
     1741 acgtagcggc cgctgccggt gacgctcaac tgctgggttc cgccggttgc ggtggtggtg
     1801 gagaagatcg tcgtccagtc cgtgccgttg ccggacacct ggatctggaa cgcccgcgcg
     1861 taggcggtct cccaggtcag ggtcacccgg cagatcgtct gcgcgctgcc gaggtcgacc
     1921 cggatccatt gcgggtcggt gaacgcgctc gaccagcggg tgccggtgtt gccgtcgacg
     1981 gcggcgctgg ccggggtgcc ggcgtgttcg gtcgacgacg cggtcacggg ccggttgagt
     2041 gccatattgg cggtgccgca cgccaggggc gcggcggtgc tggacgcggt gggcaccgcg
     2101 gtcagggtgg ccagcgcggt cgcggtgacc gcgaccagcg acagggcgcg ccgccccggt
     2161 cgggtgcggc cggcgcgcgg cgcgagaagg tcaagtctca tgatcttgcc cttacgagag
     2221 gctgcagggt tgccgccgta accggttccg gaaccggtta cgg
//
LOCUS       DATADN010000900         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1473551, whole genome shotgun sequence.
ACCESSION   DATADN010000900 DATADN010000000
VERSION     DATADN010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473551"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VHH53_11670"
     CDS             <1..218
                     /locus_tag="VHH53_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HEX2290823.1"
                     /translation="GLKGWADDSGEGRWTVEEAIDHAVPVPVITAALFARFASRQPDS
                     PAMRAVAALRQQFGGHAVRAKLAEEAQ"
     gene            238..1389
                     /gene="recF"
                     /locus_tag="VHH53_11675"
     CDS             238..1389
                     /gene="recF"
                     /locus_tag="VHH53_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX2290824.1"
                     /translation="MYVRQLQVTDFRSWEHAELDLQPGVAVLIGANGQGKTNLLEALG
                     YLSTLGSHRVATDAALVRHGAQRAIVRTVVVHAGRELTVELEISPGRANRARINRSPV
                     PRPREVLGVLRSVLFAPEDLALVRGDPGERRRFLDDLLVLRAPRLAGVRSDYDKVLRQ
                     RVALLKSAGTTRRSGAGMGDLRTLDVWDGHLARHGAELLGARLDLVAELAPHVTAAYR
                     AVAPEAPDVQLDYRSSLGDALPGEVREGGKADIDALEAAMLAELARVRLTEVERGVCL
                     VGPHRDDLELGLSGMPAKGYASHGESWSFALALRLGSYQLLRCGGAEPVLLLDDVFAE
                     LDTQRRARLAEVALAAEQVVVTAAVPADVPGELEGARFVVHEGRVHRVR"
BASE COUNT          215 a          491 c          570 g          252 t
ORIGIN      
        1 ccgggctgaa gggctgggcc gacgactccg gcgagggccg ctggacggtg gaggaggcga
       61 tcgaccacgc ggtgcccgta ccggtgatca ccgcggcgct gttcgcccga ttcgcctccc
      121 gccagcccga ctccccggcg atgcgtgcgg tcgccgcgtt gcgccagcaa ttcggtgggc
      181 acgccgtccg ggccaagttg gccgaggaag cccagtagag gagtcacagt agcccgcatg
      241 tacgtccgcc agctgcaggt caccgacttc cgctcctggg agcatgccga gcttgacctg
      301 cagcccggcg tcgcggtgtt gatcggcgcg aacgggcagg gcaagacgaa cctgctcgag
      361 gcattgggtt acctgtcgac gctgggttcg caccgggtgg ccaccgatgc cgcattggtg
      421 cgccacggag cccagcgcgc catcgtgcgc actgtggtgg tgcatgccgg acgggagctg
      481 accgtcgaac tggagatctc gcctgggcgg gccaaccggg ccaggatcaa ccgctccccg
      541 gtgccgcggc ccagggaggt gctcggcgtg ctgcgcagcg tgcttttcgc tccggaggat
      601 ttggcgctgg tccgcggcga tcccggggag cgccgccggt tccttgacga tctgctggtc
      661 ttgcgcgcac cccggctggc cggcgtgcga tcggactacg acaaggtgct gcgccagcgc
      721 gtcgcgttgc tcaaatcagc cggcacgacg cgccggtcgg gcgcagggat gggcgatcta
      781 cgcaccctgg acgtgtggga tggtcacctt gcccggcacg gtgccgagct gctcggggcc
      841 cggctggacc tcgtcgctga gcttgccccg cacgtcaccg cggcttaccg cgcagtggct
      901 ccagaggctc ccgatgtcca gcttgattac cgcagcagcc tcggtgacgc gctgcctggc
      961 gaggtgcgcg agggtggcaa ggccgacatc gacgcactgg aggcggcgat gctcgctgag
     1021 ctggcccggg tacggctgac cgaggtcgag cgtggggtct gcctggtcgg gccacaccgg
     1081 gacgacttgg agctcggtct gagtgggatg ccggccaagg gctatgccag ccatggtgag
     1141 tcctggtcgt ttgcgttggc attgcggttg ggtagctacc agctgctgcg ttgcggtggt
     1201 gccgaaccgg tgctgttgct cgatgacgta ttcgccgagc tggacaccca gcgccgcgcg
     1261 cggctggccg aggtcgcgtt ggccgctgag caggtggtgg tcactgcggc ggtgcccgcc
     1321 gatgtgcccg gcgagctcga gggtgcacgg ttcgtcgtgc acgaagggcg ggtgcaccgt
     1381 gtccgataac gccgtgccac agaacaccga taacaccgat agccccggcg cggctgtgga
     1441 caaacctgtg gacagtgtgg acaagtcgat gttgggtggg cccgcaggtc catcacagac
     1501 accacctgac ggggctcggg gtgccgac
//
LOCUS       DATADN010000901         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_853563, whole genome shotgun sequence.
ACCESSION   DATADN010000901 DATADN010000000
VERSION     DATADN010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853563"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VHH53_11680"
     CDS             <1..1418
                     /locus_tag="VHH53_11680"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX2290825.1"
                     /translation="PAWPGPGWSTSHGLANSGGTTQTLLILGATGDLTSRLLLPGLGG
                     LLTRGRVSGLSLVGASQHDWDDHRWRQLVTEAFAAGNASGAQVEAVVEGARYLQADVT
                     TEGDLRRLLGACQGRVAIYFALPPPVTVRACQALTRIDLPEGTRLVLEKPFGTDAASA
                     EALNDLLARLVPEDSVYRVDHYLGTSTVLNILGLRFANRLFEPVLNAGHVQCVDIVFD
                     ESLALEGRAGYYDGAGALVDMIQSHSLQVLALLAMEAPSTLKSRDFRDCKAQVLRATR
                     VWGDDPAVSSRRARYTAGEIAGRWLPSYVDEEGIDPARNTETLAEVVLAVDTWRWAGV
                     PFRVRAGKALSALRKDAVITFKQPPWVPTGLIGYDRPDRLRIGFDPDCLGLDMNINGP
                     GDPLVVDPITMKADFGPGDLSPYGEVLKGVFEGDPTLSVRGDTAVECWRIIEPVLRAW
                     RDDEVPLQEYPAGSAGLDDRT"
     gene            1444..>2020
                     /locus_tag="VHH53_11685"
     CDS             1444..>2020
                     /locus_tag="VHH53_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEX2290826.1"
                     /translation="MLRPQDTATRERKSLNGLWRFALDGAGQGRTEEWFRGPLAGACE
                     MAVPASFNDIAADMAVRDYFGDIWYQTTVRVPRGWDRQRIVLHFESATHRATVWVNDT
                     EVVSHEGGYTPFEADVTEHVKADDEARVTVVVNNTLSFQTIPPGVIEDTPDGKRQRYW
                     HDFFNYAGIHRTVWLYSTGHAHLTDVTIVTGI"
BASE COUNT          330 a          615 c          723 g          352 t
ORIGIN      
        1 cacccgcgtg gccaggcccc ggttggagta cgtcgcacgg tctggcgaac agcggtggca
       61 cgacgcagac actgttgatc ctgggtgcaa ccggcgactt gacctcgcgg ctgctgttgc
      121 cgggcttggg tgggctgttg accaggggcc gggtcagcgg tttgtcgctg gtcggcgcca
      181 gtcagcacga ctgggacgac caccgctggc gccagcttgt caccgaggcg ttcgcggcgg
      241 ggaacgccag cggggcgcag gtcgaggcgg tggtggaggg tgcgcgttac cttcaagccg
      301 acgtcaccac agagggcgac ctgcgacggc tgctaggcgc ttgccaggga cgcgtggcca
      361 tctacttcgc gttaccgccg ccggtcactg tgagggcgtg ccaggcactg acccgcatcg
      421 atctgcccga gggcacgcgg ctggtgctgg agaagccgtt tggcaccgac gccgccagtg
      481 cagaggctct caatgatctg ctggcgcggc tggtgccgga ggacagtgtg tatcgggtgg
      541 atcactatct gggcacgtcg acggtgctga acatcctggg attgcggttt gccaaccgtc
      601 tgttcgagcc tgtgctgaac gccgggcacg tccagtgcgt cgacatcgtc ttcgacgaga
      661 gccttgcgct ggaaggcagg gccggatatt acgacggcgc gggtgcgttg gtcgacatga
      721 tccagagcca ctcgctgcag gtcctcgcgc tgctggcgat ggaggcgccg agcaccctga
      781 agtccaggga cttccgcgat tgcaaggcgc aggtgctgcg cgccacccga gtgtggggtg
      841 acgatccggc ggtgtcgagt cgccgggcgc ggtacaccgc gggcgagatc gctgggcgtt
      901 ggctgccgtc atacgtggac gaggagggca tcgacccggc ccgtaatacc gagacgttgg
      961 cggaggtggt gttggcggtg gacacttggc ggtgggctgg ggtgcctttc cgggtgcggg
     1021 cgggcaaggc gctgagcgcc ttgcggaagg atgccgtgat taccttcaag cagccgccgt
     1081 gggtgcccac cggcctgatc ggttatgacc ggccggaccg gctgcgcatc ggcttcgacc
     1141 ccgactgtct tggcctcgat atgaacatca atggcccggg cgacccgctg gtggtcgacc
     1201 cgatcacgat gaaagcggac ttcggtcctg gcgatctctc cccgtacggg gaggttctta
     1261 aaggagtatt tgagggcgac ccgacgctct cggtgcgtgg ggacaccgcc gtggagtgct
     1321 ggcgcatcat cgagccggtg ctcagggcat ggcgcgatga cgaggtgccc ttgcaggaat
     1381 acccggccgg cagtgccgga cttgatgacc gaacctgacg actgaccgct gaggaggacc
     1441 ggcatgctcc gaccccagga cactgctact cgggaacgca agtcgcttaa cgggctctgg
     1501 cggtttgccc tcgacggagc gggacaggga cggaccgagg agtggttcag gggcccgctc
     1561 gcgggcgcat gtgagatggc ggtacctgcg agcttcaacg acatcgccgc tgacatggcg
     1621 gtacgcgact acttcgggga catctggtac cagaccaccg tgcgtgtgcc gcgcggctgg
     1681 gaccggcagc gcatcgtctt gcacttcgag tccgcgaccc accgggcgac ggtctgggtg
     1741 aacgacaccg aggtcgtctc ccatgagggc gggtacaccc ccttcgaggc cgacgtgacc
     1801 gagcacgtca aggctgacga cgaggcgcgc gttacggtcg tggtcaacaa caccctgagc
     1861 tttcagacga tcccgccagg cgtcatcgag gacacccccg atggcaagcg tcaaaggtac
     1921 tggcatgact ttttcaacta tgccggcatc caccgcacgg tgtggctgta ctcgaccggc
     1981 catgctcacc tcacggacgt cactatcgtc accggcatca
//
LOCUS       DATADN010000902         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1473616, whole genome shotgun sequence.
ACCESSION   DATADN010000902 DATADN010000000
VERSION     DATADN010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473616"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..278)
                     /locus_tag="VHH53_11690"
     CDS             complement(54..278)
                     /locus_tag="VHH53_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEX2290827.1"
                     /translation="MPPSVEALSIQYPGRMDRRKEPGIEDIGLLAKDICAALLPMADR
                     PLAVFGHSMGAAVAFELTGAWKTRDSRRFG"
     gene            complement(315..1562)
                     /locus_tag="VHH53_11695"
     CDS             complement(315..1562)
                     /locus_tag="VHH53_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2290828.1"
                     /translation="MGRICCRFGRVEPRRRAEDLVRGLASDLPIKNCWTLAEHVGAAT
                     PDGLQHLLRKAVWDHDGVRDDLRGYVVEHLGSMGGVLVVDETGDVKKGSHTVGVQRQY
                     TGTAGRIENAQVAVYLTYASDRGHALIDRALYLPESWATDAQRRRQAGVPDEVEFATK
                     PALAREMITRALDAGVSARWAAGDEVYGADPGLRDELERRGVGYVLAVACSHRVTTVA
                     GALRADTIAQRLPRRAWQRLSAGPGSKGPRYYDWAWISFPTAHAQCPGQRWLLIRRNN
                     TTGELAFYRCYAPKPVPLATLVGIAGRRWTIEESFQASKGLTGLDQHQVRRWVSWQRW
                     TVLAMLAYAYLAVLAATERARHRPPPGLIPLTCNEIHHLLNTLIVRSISSLEHHLRWS
                     TWRRRHQHRAKTSHYQRRTATQP"
     gene            1861..>2091
                     /locus_tag="VHH53_11700"
     CDS             1861..>2091
                     /locus_tag="VHH53_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290829.1"
                     /translation="MSDHLASNNDRAIEYPPCVPWCKFGRGTLDMDKHNDYCFRDIGP
                     AVIARTEDDVRYEVTASQAHLRLPVDTSAELQG"
BASE COUNT          390 a          709 c          657 g          335 t
ORIGIN      
        1 gccaatgggc ccgatgccag aatttcgcga gggcgatcgt cggcccgaga cgatcagccg
       61 aaccggcgag aatcccgcgt cttccaggca ccggtcagct cgaaagcgac ggccgcgccc
      121 atgctgtgcc cgaacacagc caggggccgg tcggccattg gcagtagagc cgcacagatg
      181 tccttggcga gcagaccgat gtcttcaatc cccggctcct tccgacggtc catccgcccc
      241 ggatattgaa tcgacaacgc ctcgacagaa ggcggcaacg cctcggctag tacgacagcc
      301 ggagattgag atcttcacgg ttgtgtcgct gttcgtcgct ggtagtggct ggttttggcc
      361 cgatgttggt ggcgtcgtcg ccaggttgac cagcgcaggt ggtgctcgag gctgctgatc
      421 gagcgcacga tcagggtgtt gagcaggtgg tggatctcgt tgcaggtcaa cggaattagc
      481 cctgggggtg ggcggtggcg ggcgcgttcg gttgcggcga gtacggcgag gtaggcgtag
      541 gcgagcatgg ccaggacagt ccagcgctgc caggagaccc agcggcggac ttggtgttga
      601 tcgagcccgg tcagcccttt gctggcctga aacgactcct cgatggtcca gcgtcggcca
      661 gcgatgccga ccagggtggc aagcggcact ggtttgggtg cgtagcagcg gtagaaggcc
      721 agctcaccgg tggtgttgtt acggcggatc agcagccagc gctgtcccgg gcactgggcg
      781 tgggcggtgg ggaagctgat ccatgcccag tcgtagtagc gcggcccctt ggagcccggg
      841 cctgccgaca ggcgctgcca ggcgcgtcgg ggcaggcgct gggcgatggt gtccgcgcgc
      901 agcgcgccag ccacagtcgt gacccggtgg ctgcaggcca cagccagcac atagccaaca
      961 ccacggcgtt ccagctcgtc gcgcagcccg ggatcagcgc catacacctc atcaccagcc
     1021 gcccaccggg ccgacacccc ggcatcgagc gcgcgggtga tcatctcccg ggccagggcg
     1081 ggtttggtcg cgaattccac ctcatcggga acacccgctt gcctacgacg ctgcgcgtca
     1141 gtcgcccagg actcgggcag atacaaggcc cgatcaatca gcgcgtgccc ccgatcgctg
     1201 gcataggtca gatacaccgc gacctgggcg ttctcgatac gaccggcggt gccggtgtac
     1261 tggcgctgca cgccgacggt gtggctgccc ttcttgacat cgccggtctc gtcgaccacc
     1321 aacaccccac ccatgctccc gaggtgttcc acgacgtagc cgcgcaggtc atcgcgcact
     1381 ccgtcgtgat cccacaccgc cttgcgcaac aagtgctgca gaccgtccgg cgtggcggct
     1441 ccaacgtgct cggcgagggt ccaacagttc ttgatcggca ggtcagaagc caacccccgc
     1501 accaaatcct ccgcacgccg acgcggctcc acccggccaa accggcaaca gatccggccc
     1561 atcaactcgt gccatcccga cccgaacacc ccgaggtcta cactggcggc cgcggccacc
     1621 gcaagatcga cttggttcca cacaaaccac ggatgatcac gcggtggccg ttctacgtca
     1681 cgccgccacg cccaagatca ccaagtccgg ctgtcgtact agtgcgctat cagccacacc
     1741 ccgaattacc cgagaaccca cgtaacagct acctcgcgcg aatcgaccca ttcaataatg
     1801 gcggtcggtg cgccaacacc ggccagggcc ttgatccacc tgatacatcc aggaggagcc
     1861 gtgagtgacc atctcgcctc caacaacgac cgggccatcg agtacccacc gtgcgtaccg
     1921 tggtgcaagt tcggtcgagg gaccctcgac atggataaac acaacgacta ctgcttccgt
     1981 gacatcggcc ccgccgtgat cgcacgaacc gaggacgacg tgaggtacga ggtcaccgcc
     2041 tcgcaagcgc atttacggct accagtggac acctcggccg agctgcaagg c
//
LOCUS       DATADN010000903         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1174289, whole genome shotgun sequence.
ACCESSION   DATADN010000903 DATADN010000000
VERSION     DATADN010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174289"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VHH53_11705"
     CDS             complement(<1..654)
                     /locus_tag="VHH53_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEX2290830.1"
                     /translation="MSDFTLAYDDFDPAQEGLREALTSTGNGYFAARGAAEWEDANAV
                     HYPGTYAHGCFNRETTILGGRPVLNEDLVNLPNWLLLKLRIEGEEAIRLDNVEVLSYR
                     HSYDVRNAVVAREIQFRDRIGRETTLRSRRFVSMANSHLAGIEWTVTPDNWSGRVEVI
                     SGLDGRVTNHMVARYRDLEGRHLNPWSPRTFGPEIIALKVQTRQSNIYIAEAARTRVF
                     "
     gene            complement(689..1561)
                     /gene="otsB"
                     /locus_tag="VHH53_11710"
     CDS             complement(689..1561)
                     /gene="otsB"
                     /locus_tag="VHH53_11710"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX2290831.1"
                     /translation="MCADAGWDRGNADASTTVTMQASPVRDLPDALRDGEQFAAKLAG
                     RRPAVFLDYDGVLTPIVDQPEDAVMSDGMREAVQALAQRCSVCVVSGRDRPVVQQLMG
                     IDNLVVAGSHGFDIWSPQEGTIVHDAATGFEDLVSHVRDRLRVEVESIPGVVVEPKRA
                     SVAVHYRRADPEHHAKVTAVVDELLDEYSGRLKATPGKMVYELQPKIDWHKGKAVLHL
                     LQTLDLDADDVVPLYLGDDITDEDAFRALAGRGVGIIVGHPDDPEVANRSTAADFVLE
                     STVEVEQFLNTLAR"
BASE COUNT          290 a          578 c          543 g          293 t
ORIGIN      
        1 gaagacacga gtgcgcgcgg cttcagcgat gtagatgttc gactgccggg tctgcacctt
       61 cagggcgatg atctccgggc cgaatgtgcg cggagaccac ggattgaggt gacgcccctc
      121 cagatcgcgg tagcgggcca ccatgtggtt cgtcacgcgg ccatcgagcc cggagatcac
      181 ttccacccga cctgaccagt tgtccggggt caccgtccac tcgataccag ccaagtggct
      241 gttggccatg ctcacgaacc ggcggctgcg cagggtcgtc tcacggccga tgcggtcacg
      301 gaactggatt tcgcgcgcca ccacggcgtt gcggacgtcg taggagtggc gataggacag
      361 cacctcgacg ttgtcaagcc ggatcgcctc ctcgccctca atcctcagct tcaacagcaa
      421 ccagttgggg aggttgacca ggtcctcgtt gaggaccggg cgaccgccga ggatggtcgt
      481 ctcccggttg aagcagccgt gggcgtaggt gcccgggtaa tggaccgcgt tcgcgtcctc
      541 ccactcggcc gctccccggg cggcgaagta gccgttaccg gtcgaggtca gggcctcgcg
      601 cagaccctct tgcgcggggt cgaagtcgtc gtaggcaagt gtgaagtccg acaacgggat
      661 accctgccct ccccgtaggt tgcacgtgtc agcgggccag tgtgttgagg aattgctcca
      721 cctcaacggt ggactccagg acgaagtcgg cggcggtcga tcggttcgcc acctccggat
      781 cgtccgggtg gccgacgatg atgccgacac cccgtccggc cagcgcccgg aaggcgtcct
      841 cgtcggtaat gtcgtccccg aggtacagcg ggacgacatc gtcggcgtcg agatccagcg
      901 tctgcagcag gtgcaggacg gccttgccct tgtgccaatc gatcttcggc tggagctcgt
      961 acaccatctt cccgggggtg gccttcagcc gcccggagta ctcgtccagc agttcgtcca
     1021 ccaccgcggt gaccttggcg tggtgctccg gatcagcgcg ccggtagtgg accgcgaccg
     1081 aggcccgctt cggctcgacc accacgcccg gaatcgactc gacctcgaca cgcagccggt
     1141 ccctgacgtg cgaaaccagg tcttcaaaac cggtcgcggc atcgtggacg atggtgccct
     1201 cctgcggact ccagatgtcg aagccgtggc tgcccgccac caccaggttg tcaataccca
     1261 tcagctgctg cacgacgggt cggtcgcggc cactgaccac gcacaccgag cagcgctgcg
     1321 ccagggcctg gaccgcctcc cgcatgccgt cggacatgac tgcgtcctca ggttgatcga
     1381 cgatcggggt caacaccccg tcgtagtcga gaaacacagc cggccggcga ccagccagtt
     1441 tcgcggcaaa ctgttcaccg tcgcgcagcg cgtcgggcag gtcgcgaacg ggcgacgcct
     1501 gcatggtcac ggttgtcgac gcgtcggcgt tgccgcggtc ccatccagca tcggcacaca
     1561 cctaatctta cgtagacggc ctccactgtg gcacagatca tcgacgctca cacctgcttg
     1621 ggcatgccat cagcaaggtg ggtggctcgc aaccacgtcg gccgtaaatg ccgcagcgct
     1681 acttccgtac ctggcacagc gccg
//
LOCUS       DATADN010000904         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_421137, whole genome shotgun sequence.
ACCESSION   DATADN010000904 DATADN010000000
VERSION     DATADN010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421137"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VHH53_11715"
     CDS             complement(<1..145)
                     /locus_tag="VHH53_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX2290832.1"
                     /translation="MTSTRVGRQARIIELVTHRAVRSQTELAALLAGEGIEVTQATLS
                     RDLD"
     gene            complement(142..1086)
                     /gene="argF"
                     /locus_tag="VHH53_11720"
     CDS             complement(142..1086)
                     /gene="argF"
                     /locus_tag="VHH53_11720"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX2290833.1"
                     /translation="MPRHFLRDDDLTPAEQREILDLADAFKADPLGHAPLAGPRSVAV
                     IFEKSSTRTRLSFQIGIAQLGGNPVIIDAATMQLGRAETIEDTSRVLSRYADAVVWRT
                     YAQTRIEAMASVSRVPVVNALTNEFHPCQVLADLQTIRERHGRLAGLTLTYLGDGANN
                     MAHSLLLGGATAGLHVRIAAPNGFEPLPHVMLAAKYRAAETAGSATVLSDPLAAVDGA
                     DVLVTDTWTSMGQESDGLDRVAPFRALQINAELLARASAGSTKKITVLHCLPAHRGAE
                     ITDEVLDGPASAVWDEAENRLHAQKALLAWLLAPERAA"
     gene            complement(1089..>1715)
                     /locus_tag="VHH53_11725"
     CDS             complement(1089..>1715)
                     /locus_tag="VHH53_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2290834.1"
                     /translation="LEPVQGENGVVIPPEGYLYAAREITTARGALLVLDEVQTGIGRT
                     GTWFAHQSVGVTPDVVTLAKGLGGGLPIGACIGIGPAAHLLQPGQHGTTLGGNPVCCA
                     AAQAVLATIASDGLLDHAARLGKEIAAGISALRHPLVGSISEAGLLIGIGLTSPVSAA
                     VATAAQDAGFLVNPAVPDRIRLAPPLVLTDAQTREFLNALPAILDRVQ"
BASE COUNT          269 a          571 c          608 g          267 t
ORIGIN      
        1 cgtcgagatc ccgggacagc gtcgcctgag tgacctcgat gccttcgccg gcgagcaggg
       61 cggccagttc cgtttggctg cgcactgcgc ggtgggtcac caactcgatg atgcgggcct
      121 gccggccaac ccgggtggag gtcacgccgc gcgctccgga gccagcaacc aggccagcag
      181 cgccttctga gcgtgcagcc ggttctcggc ctcgtcccac accgcgctgg ccggaccgtc
      241 gagcacctcg tcggtgattt cggccccacg gtgcgcgggc aggcagtgca gcacggtgat
      301 cttcttagtg cttcctgcgg aagcgcgcgc cagcaactca gcgttgatct gcagcgcgcg
      361 aaacggcgcg acccggtcga gcccgtcgct ctcctgaccc atcgaggtcc aggtgtcggt
      421 gaccaggacg tcggctccgt cgaccgccgc gagcgggtca ctgagcacgg tggcgcttcc
      481 cgcggtctcc gcggcgcggt acttcgccgc gagcatgacg tggggcagcg gctcgaatcc
      541 gttgggagcg gcgatcctga catgcagccc ggcggtggcg ccgccgagca gcaaggaatg
      601 cgccatgtta ttcgccccat cgcccagata ggtgagggtg agtccggcca ggcggccgtg
      661 ccgttcccgg atggtctgca ggtccgcaag cacctggcag gggtgaaact cgttggtgag
      721 tgcattgacc accggaaccc gcgagacgct ggccatggcc tcgatccggg tctgcgcgta
      781 cgtgcgccac accacggcgt cggcgtagcg ggacagcacc cgcgaggtgt cctcgatggt
      841 ctccgcacgg cccagctgca tcgtggcagc gtcgatgatc accgggttgc cgccgagctg
      901 ggcaatacct atctggaagg acagccgggt tcgcgtggag ctcttctcga agatcaccgc
      961 gacggatcgc gggccggcca gcggggcgtg cccgagcggg tcggccttga atgcgtctgc
     1021 caggtcgagg atctcccgct gctcggccgg ggtgaggtcg tcatcgcgga ggaagtgcct
     1081 gggcattgct actgcacccg gtccaggatc gcaggcaggg cgttgaggaa ctcccgtgtc
     1141 tgggcatcgg tgagtaccag cggtggggct agccggatcc ggtccggcac ggccgggttg
     1201 acgaggaaac ccgcatcctg cgcagcggtg gccaccgcag cggaaacggg cgatgtgaga
     1261 ccgatgccga taagcaggcc tgcctcgctg attgatccga cgagcgggtg gcgcagcgcc
     1321 gatatcccgg cagcgatctc cttaccgagt cgcgcagcat gatcgagcaa gccatccgag
     1381 gcgatggtcg ccagcacggc ctgggcggcc gcgcagcaca ccgggttgcc accgagagtg
     1441 gtgccgtgct ggcccggttg tagcagatgc gcggcaggtc caatgccgat acatgcaccg
     1501 atcggaagcc ccccgccgag gcccttggca agcgtcacca cgtcgggggt cacgcccacg
     1561 gactgatggg cgaaccaggt gccggtccgg ccgatgcccg tctgcacctc gtctagcacc
     1621 agcagtgcgc cgcgcgcagt ggtgatctcc cgagcggcgt acaggtaacc ctccggaggg
     1681 atcacgacac cgttctcacc ctggacgggc tcgag
//
LOCUS       DATADN010000905         9077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_421242, whole genome shotgun sequence.
ACCESSION   DATADN010000905 DATADN010000000
VERSION     DATADN010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421242"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..954)
                     /locus_tag="VHH53_11730"
     CDS             complement(28..954)
                     /locus_tag="VHH53_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2290835.1"
                     /translation="MTRLHRRSRHSIVAGAALLALLIASGCGSDRTNTDGPAASAATF
                     DQALHDRLPAGILTRGSVRVGGGDPYPPASFFAPDGRTIVGFEPDLAAAMGRVLGVRF
                     EWVNVDFSDLLPQLNSDKIDIAMASMTDTAEREKHADFVNYFQAGTSIIVQRGNPHGI
                     STLKDLCGKVVAVEEETTQVDLLQRTQKGCVGKRIDIRTYGDNANALVQLRTGRAVAV
                     LSDYPPAVHVSTDQRTSAHYQLAASTQYEPSPYGIAVAKDQTELRNVVRDAMERVMRS
                     GDYADVLQRWGVPSGALESATINAGGTAASAG"
     gene            complement(951..2930)
                     /locus_tag="VHH53_11735"
     CDS             complement(951..2930)
                     /locus_tag="VHH53_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2290836.1"
                     /translation="MPHVGSRLFAAYAAASLVPVVLLGAVLVRGAQQEALESGLEQGR
                     AQAAVIEEMAIAPALTGTDLDQQLTAEERQRLQGATDLAIFHGSVIRLRLRGFRGRVV
                     FSDNGSTAGALPTSHPAFGAAAVGRTDVAIVQDPDGAHGQVIRVLQPVVAKATGQSTG
                     VLELYLPYSTIAKHVQAQMRRTYWRLAAGLTALYAVLAFIAWSTTRRLRRHATRHQHE
                     ALHDALTGLPNRKWFREQAEDAVERGQRDAHGAIVLVDLDRFKDVNDTLGHHAGDELL
                     RVVAQRLPASLRTDDTVARLGGDEFGMILPGIPDAAHALQLLARVREELATPIVLDGV
                     PLSIEASFGVALYPAHGNTVELLLQHADAAMYQGKRGTSGIVVYESATATHPTQWLIV
                     QGELRRALEHHELVLHYQPKVELATGQICGVEALVRWDHPQRGLLSPAEFLPAVEQSS
                     LIDPLTRWVLRRALTDHAAWTALGLPWPVAVNVSARNLEVEVFPGSVAELLAQFGVPA
                     NQLHLEITETALAADAVAAAQTVAALAGQGIAISIDDFGEGYTSLSQLRNLPIAELKI
                     HQSFVMDLERDERDRAIVRSLIGLADGLGCRVTAEGVETADAANWLAAAGCECAQGYL
                     FSKPLPYDELVNRYHRPADTVQNEQSHTTLEANWS"
     gene            complement(3179..5062)
                     /locus_tag="VHH53_11740"
     CDS             complement(3179..5062)
                     /locus_tag="VHH53_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2290837.1"
                     /translation="MLARQLRRLSLDPATPPDLTDWQRFLERVERSYAEADAERYTSE
                     RLMELSSQEMAALHQQLRDHDQRRFQALVAGSSDTTIVLDAAGKVTYVSSSATRVLHL
                     AADQLQGQPMVAFVGRHVHPDDAGPLTATLQHLLDTAGGEESFEFRLRRPDGTWRDVE
                     GLGRNLLGDEAVRGVLFTLRDVSERKQLERALTRQAFTDQLTGLPNRALLRDRAEQAL
                     RLAGRQGLTAALLLLDLDRFKEVNDTLGHHHGDLLLQQVAERLHGSLRGSDTVARLGG
                     DEFAVLLPEIASVQEATVVADKLSAAIEAPFTVDGFTLDVDASIGVAVYPDHASDANE
                     LLQRADVAMYAAKATHAGYTVYDPYMDQHNPRRLSLLGQLRRALAAGELVVHYQPKAD
                     VRSGRILGVEALVRWQHPEHGLLGPGEFVPLAETTGLIRPLTAHVLDAALRQCRAWRD
                     AGRALSVAVNLSTRCLLDLTLPDQITALLEDTAVAPERLVLEITESSIMTDPTRALEI
                     LNRLHTLGVQLAIDDFGTGYSSMAYLKSLPVDELKVDRSFVKHLRHNQSDAVIVRSTV
                     DLGHNLGLRVVAEGVEDQATWQELATLGCDSVQGYYLARPMPAAELAAWLATQPQPAA
                     SHD"
     gene            complement(5077..6213)
                     /locus_tag="VHH53_11745"
     CDS             complement(5077..6213)
                     /locus_tag="VHH53_11745"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX2290838.1"
                     /translation="MDVRSLAWEPSSGWSAPLPSELDGPSTLVLAFGSPPEEVATARL
                     AELAAALPQAQLAGCSTAGEILDRRVAEGEMSVAVARFEHTTLRRAEAAVAAPTDSRQ
                     AGRELAGTLVGPGLRGVLVLSDGLGVNGTELLNGVKEVLPAEVVVSGGLAGDGDRFAA
                     TWVVAQGRPTAGVVSAIGLYGDRVRIGHGSWGGWDSFGVERQVTRSDGKVLYELDGEP
                     ALALYKRYLGERAAGLPATALLFPLALCADDGGAGLVRTVLGVDEANQSLTFAGDLPQ
                     GSRVQLMRANFDRLVEGAATAGQMAYPAEDSRGGCLAIAISCVGRRLVLGERTEEEVE
                     AVLDSLPPASSQVGFYSYGEISPTATGPCELHNQTMTLTTLVEA"
     gene            complement(6647..7231)
                     /locus_tag="VHH53_11750"
     CDS             complement(6647..7231)
                     /locus_tag="VHH53_11750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2290839.1"
                     /translation="MAVAIVLETHSVTTDNEAGVATGWLDGRLSERGRIRATELGRRR
                     RNDGVAAVFASDLGRAVETVELAFGGSGIPVHRDARLRECNYGTWNGMPVSRLERERT
                     RRIDQPFPGGESYLQVVGRVAGFLEELARDWDGARVVVVGHSATRWALDHLLAGAVLH
                     EVMVAPFAWQEGWLYTLPTGWQRPGLVASSGDPS"
     gene            complement(7380..8237)
                     /locus_tag="VHH53_11755"
     CDS             complement(7380..8237)
                     /locus_tag="VHH53_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2290840.1"
                     /translation="MRRIVTMVVLTVLLGSCSGAGDSANNSAAPGPATTQPAATGNID
                     GSFDVGGHKLHLRCEGKSAPGSPTVVYLHGLGGDGSDVKSISAPLASRVRVCTYDRLN
                     VGGSDSDPGRHTGADSVRDLHALLDAAGVAGPYLLVGFSFGGLLAIMYAGTYPDQVMG
                     LVSLDGSLPTDDQVDQLIPKDERAQVMAEQDANQERVEFYRTVDQAKALVAKVPDVPI
                     TYLAARPVELPPNWPVKRMRAFIAAKQAQFTKAVPNGRLVEVQSSHDIDLEQPELVIK
                     EINRILKTA"
     gene            8815..>9077
                     /locus_tag="VHH53_11760"
     CDS             8815..>9077
                     /locus_tag="VHH53_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2290841.1"
                     /translation="MLLVGINWAERHHDACLMAADGQVLAAEQIADGVAGVARLHELI
                     AQARQRPGRGGGRDRDRPGVAGGGLVAAGYQVVAVNPLAASRYR"
BASE COUNT         1369 a         3094 c         3231 g         1383 t
ORIGIN      
        1 cgctctggag ggggagcccc catggggtca gccggcgctg gcggcggtgc cccctgcgtt
       61 gatggttgcg gactccaggg cgccgctggg gacgccccag cgctgcagta cgtcggcata
      121 gtcgcccgag cgcatgacgc gctccatagc gtcacgcacg acattgcgta actcggtctg
      181 gtccttcgcc acggcgattc cataggggct tggctcgtac tgggtgctgg ccgcaagctg
      241 gtagtgcgcg cttgtccgct ggtctgtcga cacgtggacg gccggcggat agtcgctgag
      301 gacggcgacg gcacggccgg tccgcagctg gacgagggcg ttggcgttgt caccgtaggt
      361 ccggatgtcg atccttttcc ccacgcagcc cttctgggtg cgctggagca ggtccacttg
      421 ggtggtctcc tcctcgacgg ccacgacctt gccgcacagg tccttcagcg tgctgatccc
      481 gtgcgggttg ccgcgttgga cgatgatgga ggtgcctgcc tggaagtagt tgacgaagtc
      541 tgcgtgcttc tcgcgttcgg cggtatcggt catggaggcc atggcgatgt cgatcttgtc
      601 gctgttgagc tgcggcagca ggtcgctgaa gtccacgttg acccattcga accgcacccc
      661 gagcacccgt cccatcgcgg cggcgaggtc gggctcgaag ccgacaatgg tgcggccgtc
      721 gggggcgaag aaggaggccg gagggtatgg atcacctccg cccacacgaa cgctgccgcg
      781 cgtgaggatg ccggccggta ggcggtcgtg caacgcctgg tcgaaggtgg cggccgaggc
      841 ggctggcccg tcggtgttgg tgcggtcgct cccgcatccc gaggcgatga gcaacgccaa
      901 cagcgcggca ccggcgacga tggaatgtcg cgaacgacgg tgcagacggg tcatgaccag
      961 ttggcctcca gcgtcgtgtg cgattgctcg ttttggacgg tgtcggcagg tcggtggtag
     1021 cggttgacca gttcgtcgta gggcagcggc ttggagaaga gatagccctg ggcgcattcg
     1081 cagcctgctg cggcgagcca gttggcggca tccgcggttt ctacgccttc ggcggtcacg
     1141 cggcacccca gcccgtcggc gagcccgatg agcgagcgca cgatggcacg gtcccgctcg
     1201 tctcgctcca gatccatgac gaacgactgg tggatcttca actccgcgat ggggaggttg
     1261 cgcagctgtg acaggcttgt gtagccctcg ccgaaatcgt cgatgctgat ggcgatcccc
     1321 tggcccgcca gcgccgcgac agtctgcgcg gcggcgaccg cgtccgccgc caaggcggtc
     1381 tcggtaatct cgagatggag ctggttcgcc gggacgccga actgggccag cagctccgcc
     1441 accgacccgg ggaacacctc gacttcaagg ttgcgcgccg acacgttcac tgccaccggc
     1501 catggcagtc ccagcgccgt ccaggcggcg tggtcggtga gggcacgccg cagaacccac
     1561 ctcgtgaggg ggtcgatgag gctggactgc tccacggccg gcaggaactc ggctggagac
     1621 agcagcccgc gttgcgggtg gtcccagcgc accagagcct cgacgccgca gatctggccg
     1681 gtggccagct ccaccttcgg ctggtaatgc aggaccagct cgtggtgttc cagggcccgc
     1741 cgcagctcgc cctgcacgat gagccactgg gttggatgcg tggcggtcgc cgactcatag
     1801 acgacgatcc cagaggttcc gcgctttccc tggtacatcg cggcgtcggc gtgctggagg
     1861 agcagttcga cggtgttgcc gtgggcgggg taaagcgcga ccccgaagct cgcctcgatg
     1921 ctgagcggga ccccatcgag cacgattggg gtggccagct cctcacgcac ccgggcgagc
     1981 agctggaggg catgcgcggc gtcggggatg ccaggcagga tcatgccgaa ctcgtcgccc
     2041 cccagccgcg cgactgtgtc atcggtgcgc agggacgcgg gcagccgttg ggcaacgacg
     2101 cgcagcagct cgtcgcccgc gtggtggccg agggtgtcgt tgacgtcctt gaagcggtct
     2161 aggtcgacga ggacgatggc gccgtgagcg tcgcgctggc cgcgctcgac ggcgtcctct
     2221 gcctgttcgc ggaaccattt gcggttgggt agtccggtca gcgcgtcgtg tagggcctcg
     2281 tgctggtgcc gggtggcgtg gcggcgcagg cgacgggtcg tcgaccaggc gatgaacgcc
     2341 agcacggcat acagcgcggt gagcccggcg gccaaccgcc agtaggtgcg gcgcatctgc
     2401 gcctggacgt gcttggcgat ggtgctgtac ggcagataga gctccagcac gccggtcgac
     2461 tggccggtgg ccttggcgac gaccggctgc agcacgcgaa tcacctgacc gtgggcgccg
     2521 tcagggtcct ggacgatggc gacgtcggtg cggccgaccg cggcggcgcc gaaggcagga
     2581 tgtgaggtcg gcagggcgcc ggcggtggat ccattgtcgg agaacacgac ccgtccccgg
     2641 aagccgcgga ggcgaagccg gatgaccgaa ccgtggaaga tggccaggtc ggtggcgccc
     2701 tggagccgtt ggcgttcctc ggcggtcagc tgctgatcga gatcggtccc ggtgagggcc
     2761 ggcgcgatcg ccatctcctc gatcaccgcg gcttgcgccc gaccctgctc cagcccgctc
     2821 tccaacgcct cctgctgggc accacgcacc agcacggcgc cgagcagcac caccgggacc
     2881 aggctggccg cagcataggc cgcgaacaag cgcgaaccca catgcggcat gctcttcgag
     2941 ggctcctgca tacctgtttc tatcggcccc ccgaccaggc gcatgagggc gcgggtctgg
     3001 tccgctgcac ccgtcaccca gcgtgcagag ccgggtagcc ggcgacccgt cggttggtcc
     3061 cggggttatc gaagttggag cgcgcgtgag cctcagccac gaccttgcgg agcaccaggc
     3121 tcgtaatcgg atggtcgccg ctcgcggtcc ggcttggcga cgccgcctac tcgtgctgtc
     3181 agtcgtggga ggcggccggt tgcggctgtg tggccagcca cgcggccagc tcggctgcgg
     3241 gcatcggcct agcgaggtag tagccttgca cgctgtcaca gcccagggtg gccagctcct
     3301 gccaggtcgc ttggtcctcg acgccctcgg cgaccacccg caggccgagg ttgtggccga
     3361 gatccaccgt cgagcggacg atcaccgcgt cgctctggtt gtggcgcagg tgcttgacga
     3421 acgagcggtc gaccttcagc tcgtccaccg gaaggctctt gaggtacgcc atggaggagt
     3481 agccggtgcc gaagtcgtcg atcgccagct gcacgccaag cgtgtgcagg cggttgagga
     3541 tctccagggc ccgggtcgga tcggtcatga tcgagctctc ggtgatctcc agcaccaggc
     3601 gttcaggggc gaccgcggtg tcttcaagca acgcggtgat ctggtcgggc agggtgaggt
     3661 cgagcaggca gcgggtggac aggttgaccg cgactgacag cgcgcggccg gcgtcccgcc
     3721 aagcgcggca ctgacgcaag gcggcgtcca agacatgggc ggtgagcgga cggatcagcc
     3781 cggtggtctc ggcgagcggg acgaactcgc ccgggccgag caggccgtgc tcggggtgtt
     3841 gccagcgcac cagcgcctct acgccgagaa tccgccccga gcgcacgtcg gccttgggct
     3901 ggtagtgcac caccagctcg ccggccgcca aggcacggcg gagctggccg agcaggctga
     3961 gccgacgcgg attgtgctgg tccatgtagg ggtcatagac ggtgtacccg gcgtgggtgg
     4021 ccttggcggc gtacatggcc acgtcggcac gttgcagcag ctcgttggcg tcactggcgt
     4081 ggtcggggta gacggccaca ccgatgctgg cgtcgacgtc cagggtgaac ccgtccacgg
     4141 tgaacggcgc ctcgatcgcc gcgctgagct tgtcagcaac caccgtcgcc tcctggacgc
     4201 tggcgatttc gggcaagagc acggcgaact cgtcgccgcc cagacgcgcg accgtgtcgc
     4261 tgccgcgcag gctgccgtga aggcgctcgg cgacctgctg cagcagcagg tcaccgtggt
     4321 ggtggccgag ggtgtcgttg acctccttga agcggtccag gtccagtagc agcagcgcgg
     4381 cggtcagccc ctgccggccg gcgagacgca gcgcctgctc ggcgcggtcc cggaggaggg
     4441 cacggttggg cagcccggtg agctggtcgg tgaacgcttg gcgggtcagc gcgcgttcca
     4501 gctgcttgcg ctcgctcacg tcgcgtaggg tgaacagcac cccacgcacc gcctcgtcgc
     4561 cgagaaggtt gcggcccaga ccctcgacat cacgccaggt gccgtccggc cggcgcaggc
     4621 ggaactcgaa cgattcctca ccgcccgcgg tgtcgagcag gtgctggagc gtggcggtga
     4681 gcgggccggc gtcatcgggg tgcacgtggc gaccgacgaa cgccaccatc ggctgcccct
     4741 gcagctggtc ggcggccagg tgcagcacgc gggtggccga ggagctgacg taggtgacct
     4801 tgccggccgc gtcgagcacg atggtggtgt cggaggagcc ggccaccagc gcctggaagc
     4861 ggcgctggtc gtggtcgcgg agctgctggt ggagggcggc catctcctgg gaggacagtt
     4921 ccatcaagcg ctcgctggta tagcgctcgg cgtccgcctc ggcataagag cgctcgaccc
     4981 gctccaagaa ccgctgccag tccgtcaaat ctggcggggt ggcggggtcc agcgacagcc
     5041 ggcggagctg tctggccagc acccggtgca cgctggctag gcctccacca gggtggtcag
     5101 ggtcatggtc tggttgtgga gttcacaggg gcccgtggcg gtgggggaga tctccccata
     5161 ggagtagaac ccgacctggc tgctggcagg cggcaggctg tcgaggaccg cctcgacctc
     5221 ctcctcggtc cgttcgccaa gcaccaggcg gcggccgacg cagctgatcg cgatggccag
     5281 gcagcccccg cgggaatcct cagcagggta ggccatctgt cccgccgtgg cggcgccctc
     5341 caccagccgg tcgaagttgg cccgcatcag ctggacccgg ctgccctgag gcagatcccc
     5401 ggcgaaggtc agggactggt tggcctcatc cactcccagt acggtgcgga ccagcccggc
     5461 cccgccatcg tcggcgcaga gggccaacgg gaagagcaga gccgtggccg gcagcccggc
     5521 cgcccgctcg cccagatacc gcttgtacag cgccagcgcg ggctcgccgt ccagctcgta
     5581 cagcaccttg ccgtccgagc gggtcacctg ccgctccacc ccgaagctgt cccagccgcc
     5641 ccacgagcca tgcccgatcc ggacccggtc gccgtacagg ccgatcgccg agaccacccc
     5701 ggcggtcggg cggccctggg ccaccaccca ggtcgcggcg aatcggtcgc cgtcgccggc
     5761 cagcccaccg ctgacgacga cctcggccgg cagcacctcc ttgacgccgt tgagcagctc
     5821 ggtgccgttg accccgagcc cgtccgacaa caccagcacc ccacgcagtc ccggccctac
     5881 cagcgtccca gccagctcgc ggcccgcctg ccgtgagtcg gtcggggccg cgacggccgc
     5941 ttcggcgcgc cggagggttg tgtgctcgaa ccgggccacg gccacactca tctcgccctc
     6001 ggccacccgc cggtccagga tctccccggc ggtcgagcag ccggccagct gggcctgcgg
     6061 caacgccgcc gcgagctcgg ccagccgcgc cgtcgccacc tcctcgggtg gcgacccgaa
     6121 cgccaatacc agcgtcgaag gcccatccag ctcgctgggc agcggagccg accagccgct
     6181 cgacggctcc catgcaagcg accgcacgtc catgccgagc tcctattaac cggccgggtc
     6241 caggtcaccg cccggcgttc gtcaggatca tcgacacgac cagtaccgac atgagccttc
     6301 cagccgttgc ccttggaaac ggggtgcggt ggcgccgagg caccggctct gccgagcgct
     6361 acccacagcg tcgacacggc gccaggcgga gcggcgggta tgtcattctc agagtcattc
     6421 caccaggcac aaccagcgag acaggagacg accaggcgga caggacagcc gacctgacca
     6481 gcgtgaacga cactagaggg tatccggtgg actgttgtga accgacgcat actcgctcgg
     6541 tcgcaggttc gaggcctgcc agtccaccca acgccgcagg cagaggcctg cggcacttac
     6601 tgggcagctc ttcgctcctg gaacgctggg aacgctcgac cgtcggctac gacggatcac
     6661 ctgagctggc taccaggccc ggtcgctgcc aacccgtagg cagggtgtag agccagcctt
     6721 cttgccaggc gaatggggcc accatgacct cgtggaggac ggcgccggcc agcaggtgat
     6781 ccagcgccca gcgggtcgcc gaatggccga ccaccaccac ccgggccccg tcccaatcgc
     6841 gcgccagctc ctccaggaag ccggccaccc ggcccacgac ctggaggtag ctttccccgc
     6901 ctgggaacgg ctggtcgatc cgccgtgtcc gctcgcgctc cagccgggac accggcatgc
     6961 cgttccacgt cccgtagttg cattcgcgca gccgagcgtc tcggtggacg ggaatgcctg
     7021 atccgccgaa ggcaagctcc accgtctcca ccgcgcgccc gagatccgag gcgaacaccg
     7081 ccgcgacccc atcgttgcgg cgtcgccgac cgagttcagt ggcccgaatc ctgcctcgct
     7141 ccgacagccg gccgtccaac cagccagtcg caacacccgc ctcgttgtcg gtcgtcacgg
     7201 agtgcgtctc gaggacgatc gcaacggcca cgaccacctc cacagcagca acgcagggac
     7261 gtgacggcca tcaagcttgc ccagccacct ggtgggtaga tatcgcaacc tcccatatcc
     7321 ctggttgccg gtgcgggagc ccctcgcccg gcgggtgccg ctgcgcggag ggcggcgcgc
     7381 taggccgtct tgaggatccg gttgatctcc ttgatgacga gctccggctg ctccaggtcg
     7441 atgtcgtggg aggactggac ctcgaccagc cggccgttgg gcacagcctt ggtgaactgg
     7501 gcctgcttgg cggcgatgaa cgcccgcatc cgcttgaccg gccagttcgg gggcagctcg
     7561 accggtcggg cggccaggta ggtgatcggc acgtccggca ccttggcgac cagcgccttg
     7621 gcctgatcca cggtgcggta gaactccacc cgctcctggt tggcgtcctg ttcggccatt
     7681 acctgggcac gctcatcctt gggaatcagc tggtcgacct ggtcatcggt gggcaggctg
     7741 ccgtccaggc tgaccagccc catgacctgg tcggggtagg tgccggcgta catgatggcc
     7801 agcaggccac cgaacgagaa cccgaccagc aggtacggcc cagcgacacc ggcggcgtcc
     7861 agcagggcgt gcaggtcgcg gacgctgtcg gcgccggtgt ggcggcccgg gtccgagtcg
     7921 ctgccgccca cgttcagccg gtcgtaggtg cagacccgga cccggctggc cagcggggcg
     7981 ctgatcgact tgacatccga gccgtcgccg cccaggccat gcaggtacac cacggtcggt
     8041 gaccctgggg cgctctttcc ctcacagcgc aggtggagct tgtggccgcc gacgtcgaag
     8101 ctgccgtcga tgttgcccgt ggccgccggc tgggtggtcg ccggtcccgg ggccgccgag
     8161 ttgttggcgc tgtcgccggc gcccgagcag ctccccagca ggaccgtcag gaccaccatg
     8221 gtgacgattc ttcgcacact ggcctcccca ttccccgctc gacttgccct gacgtcgaca
     8281 cgatcggtcg tgaccgtccc agcagcgtta cgagcccgga ggcagatggc gaaccagtcc
     8341 ggcacgcgtc aaccaacggt gagccgcatc cgcagtgcca aggatgctgc aggccagcgc
     8401 gggagcgcct tcgccgggcg ggtgccaggg cgcggagggc ggaacgcccg acccgtgtcc
     8461 tcgggaccat ccggcccggc ccggggtcaa ggtggagcgc ccgcaggccg ccagctcgcc
     8521 ctcgggccgc cctagctggc cgcagctggc ggccgaggac ggacgaccta atggccatgc
     8581 actagccctt ggatgggagg gttgctgtac ggcagagccg gcgggtgagc accacacgaa
     8641 acgactgggc gcagctgagc cctttcgtca agaccgtgcc gtgcccccgc cggccaggcg
     8701 aggagaccga tggctggtgg gatgacgtgc cgacgctcga gcactgctcc attaggttgc
     8761 cgcagctctc ctccaggcca ggagcgaacc tcacacaaga agggagagct ggcggtgttg
     8821 ctggtcggga ttaattgggc tgagcgccac cacgatgcgt gcctgatggc cgccgacggc
     8881 caggtgctgg ccgccgagca gatcgccgac ggagtggccg gggtggcccg gctgcacgag
     8941 ctcatcgccc aggcacgcca gcgacccggc cggggtggtg gtcgggatcg agaccgaccg
     9001 ggggttgctg gtggggggct ggtcgcggct gggtaccagg tggtggcggt gaatccgttg
     9061 gcggctagcc gctatcg
//
LOCUS       DATADN010000906         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1474045, whole genome shotgun sequence.
ACCESSION   DATADN010000906 DATADN010000000
VERSION     DATADN010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474045"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VHH53_11765"
     CDS             complement(<1..244)
                     /locus_tag="VHH53_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX2290842.1"
                     /translation="MFLGTHTPRLDDKGRLTLPAKFRDALAGGLMVTKGQDHCLYVFP
                     RAEFEEMARKVAQAPFTNEAVRAYQRYLFAGTDEQRP"
     gene            643..>1505
                     /locus_tag="VHH53_11770"
     CDS             643..>1505
                     /locus_tag="VHH53_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2290843.1"
                     /translation="MQPPQLVDVHAAAQRIAVNVERVLVGKPDAVRTALVTLLAEGHL
                     LIEDVPGVGKTSLAKALARSIDCTVNRIQFTPDLLPSDITGVSVFDRSTGGFDFRPGP
                     IFANVVVGDEINRASPKTQSALLECMEEHQVTVDGRSYPLSGPFMVIATQNPVEMEGT
                     YALPEAQRDRFTARVSLGYPDAAAELAMVDEHAADDPLATLAPVSTAAEVTALCQAVR
                     LIHVATEVRRYVVDVVGATRQLPELRLGASPRATLQLIRTSRAAAALVGRDFVVPDDV
                     QAVAPQVLSHRL"
BASE COUNT          239 a          519 c          491 g          256 t
ORIGIN      
        1 ccgggcgttg ctcatcggtg ccggcaaaca ggtaacgctg gtaggcccgc accgcctcgt
       61 tggtgaatgg cgcctgcgcc accttgcggg ccatctcctc gaactcggcg cgcgggaaga
      121 cgtagaggca gtgatcctgt cccttggtaa ccatcaaccc ccctgcgagc gcgtcgcgga
      181 acttcgccgg cagcgtcaac cggcccttgt cgtcgagccg gggggtgtgc gtacccagga
      241 acacaggtca cccacctccc ccagcagccg gtggctggcc atggagtgcc cttgtactcc
      301 actgtccgcc acagtacccc actttccgcc actgtcaacg cccaactacg ttgcgctgtg
      361 gctgcagaac gcggtcagcg cacctcagct gcgtgcaaca aggtggggga ctaccaaagc
      421 cagggagcga atctccggtc tccagtcccg cacctccgcc catacaccca cctcgtcccc
      481 ctacggcgcc ccctccgtgg gggaaagtgg tggaccttcg tcacgtggat gcctgtcgcg
      541 gccgaagcac attcgggtcg cccacatgga tgcttgttac tgcccgggcg gttaaagtcg
      601 gcaaatgatc aggaggtcgc cgtgagcgtg ccgcccaccg ggatgcagcc gccgcagttg
      661 gtggacgtgc acgctgcagc gcagcgcatc gcggtcaacg tcgagcgggt gctggtcggc
      721 aagcccgacg cggtccggac cgcgttggtc acgcttctcg ccgaggggca cctgctgatc
      781 gaagacgtgc cgggagtggg caagacgtca ttggcgaagg cgctggcccg gtccatcgac
      841 tgcacggtca accggatcca gttcaccccg gaccttttgc ccagcgacat caccggggtg
      901 tcggtttttg atcgcagcac tggaggcttc gacttccggc caggcccgat cttcgccaac
      961 gtggtggtgg gcgacgagat caaccgggcc tcgcccaaga cccagtccgc gctgctggag
     1021 tgcatggagg agcaccaggt caccgtcgat gggcgcagct acccgctgag cggccctttc
     1081 atggtgatcg cgacccagaa cccggtcgag atggagggca cctacgcgtt gccagaagcc
     1141 caacgcgatc ggttcaccgc ccgtgtctcg ctgggctacc cggatgccgc cgcggagctt
     1201 gcgatggtgg acgagcacgc ggctgatgac ccgttggcca cgttggcgcc ggtgtccacc
     1261 gcagccgagg tgacagccct gtgccaggcg gtgcgcctga ttcacgtggc caccgaggtg
     1321 cgccgctacg tcgtggacgt ggtcggcgcc actcggcagt tacccgagct tcggctgggc
     1381 gcctcgccac gggccacgct gcagctcatt cgcaccagcc gcgcagcagc tgcactcgtc
     1441 ggccgtgact tcgtggtgcc cgacgatgtg caggctgttg ccccgcaggt gttgtcgcac
     1501 cggct
//
LOCUS       DATADN010000907         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1305664, whole genome shotgun sequence.
ACCESSION   DATADN010000907 DATADN010000000
VERSION     DATADN010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305664"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VHH53_11775"
     CDS             complement(<1..562)
                     /locus_tag="VHH53_11775"
                     /inference="COORDINATES: protein motif:HMM:NF042633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator"
                     /protein_id="HEX2290844.1"
                     /translation="MDQIVDRASRPDFERWSEQVHRCGYCSRPVRLRGRVVYQSAHDR
                     RSAYSTEDEPDRVLMIRCGNRRAAVCPSCSWEYAGDLWQLLYAGAAGGRKGVPDSIRS
                     HPLVFATLTAPGFGAVHTTRADRNGKHARCRPARTKPTLCRHGRPSWCNAIHGEDDPR
                     LGQPICPDCYDYLAHAAFNWHAPELWR"
     gene            complement(803..>2112)
                     /locus_tag="VHH53_11780"
     CDS             complement(803..>2112)
                     /locus_tag="VHH53_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX2290845.1"
                     /translation="ITASAVVGMLVGWRLFDLASFDAWAGRYLRAWWLRWTVYAPKLP
                     AWLNACGLGIKHDAAPVVVALTPLGRGLRWRRQQVTTQLPTVLGVRSGASWDEVRVRL
                     VPGQTPEDFDVAARALACARGVARCQVRELAPNVISIDFQRRNLLADPVTCPEPDTLA
                     RVPGSAIYLHQVVAGRTEYGRDWHVSLTGPASHTMTAGASGAGKNSVMWRPLVSIAPA
                     IRDGLVRVSGIDPKGMELAYGRGIFARYAVTGAEALDVLDELVEVMTARKTDFAGHVR
                     TVPITRGHPLELLEFDEIGALTKYTDRKTRDAITERVALLTTQGRALGVTVRGYVQEP
                     TKDTVPVRELFPRRICLRVTAKSHVSMVLGDGAYERGAWANRITEDEAGVGYVFGEGI
                     REPLRIRAGWVPDQTIKHLEAFVTSPLTYDRAVLPLPAGGVRA"
BASE COUNT          349 a          691 c          745 g          327 t
ORIGIN      
        1 ggcgccacaa ttccggggcg tgccagttga acgccgcgtg cgctaggtag tcgtagcagt
       61 cggggcagat cggctggccc agccgcggat catcctcacc atggatcgcg ttacaccagc
      121 tgggccgccc atgccgacac aacgtgggct tggtccgtgc cgggcggcac cgcgcgtgct
      181 tgccattccg gtcggcgcgg gtggtgtgta ctgccccgaa gccgggggcg gtcagcgtgg
      241 cgaacaccag tggatgggaa cggatcgagt ccgggacacc cttgcggcca ccggcagcgc
      301 ccgcgtagag caactgccac aggtcgccgg cgtactccca cgagcacgac gggcacactg
      361 ccgcccgccg gttgccgcac cggatcatca gcacccggtc gggctcatcc tcggtggagt
      421 acgccgaccg ccgatcatgc gccgactggt agacgacgcg accccgcaac cgcaccggcc
      481 gggagcagta gccgcagcgg tgcacctgct cggaccagcg ctcgaagtcc ggtcggctag
      541 cccgatcaac gatctggtcc atcacctggg gtgcagctca acaggcgtgg tcatcgaccg
      601 tctctcgaga gggtcggccg aggccagcag ctcagcagcg atacgggcca cggttgcggg
      661 aacacctgcg ctgagaggca cgcctggtgc gctctgcttg tcagcgccag gaacgccgat
      721 tccgggagag cgtgatcgat caggtccgcg gcgagtaggc ccatcgcctg cgggaccgtt
      781 tcggcgaggg gtggagtgct gttcatgccc tcacaccccc tgcgggaagc ggcaggactg
      841 cccggtcgta cgtgagggga ctggtgacga aggcttcgag gtgtttgatc gtctggtcag
      901 gcacccatcc ggcgcggata cgcaggggtt cgcggatgcc ctctccgaag acgtagccga
      961 cgccggcttc gtcttcggtg atgcggttag cccacgcacc ccgttcgtag gcgccatcac
     1021 cgagcaccat gctcacgtgg gatttggcgg tcacgcgcag gcagatccgg cgggggaaca
     1081 actcccggac tggcacggtg tctttggtgg gttcttgcac gtagccgcgg acggtgacac
     1141 ccagggcgcg gccctgggtg gttaataggg cgacgcgttc ggtgatggcg tctcgggtct
     1201 tgcggtcggt gtatttcgtg agtgctccga tttcgtcgaa ctccagcagt tccaaggggt
     1261 gtccacgagt gatgggcacg gtgcgcacgt ggccggcgaa gtcggttttg cgggcggtca
     1321 tcacttcgac gagctcatcg aggacatcca gggcctcggc gccggtgacg gcgtagcggg
     1381 caaagattcc tcgtccgtag gccagttcca tgcccttggg gtcgatgccg gagacccgga
     1441 ccagcccgtc gcggatggcg ggggcgatgg agaccagcgg gcgccacatg accgagtttt
     1501 tgcccgcgcc gctggccccg gcggtcatgg tgtggctggc cgggccggtc agcgacacgt
     1561 gccagtcgcg gccatattcg gtgcgcccgg ccaccacctg gtgcagataa atcgcgctac
     1621 cgggcacgcg ggccagggtg tccggctcgg ggcaggtcac cgggtcggcg agcaggttac
     1681 ggcgctgaaa atcaatcgag atcacattgg gggcgagttc gcggacctgg cagcgcgcta
     1741 ccccacgggc gcaggccaac gctcgggcgg cgacatcgaa gtcctccggt gtctggccgg
     1801 gcactagccg gacgcggacc tcatcccacg aggcacccga acgcacaccc aacactgtgg
     1861 gcagctgcgt ggtgacctgc tggcggcgcc aacgaagccc gcggcccagg ggagtcaggg
     1921 ccaccacgac cggcgcggcg tcgtgcttga tgcctaggcc acaggcgttc agccacgcgg
     1981 gcagtttggg agcgtacacc gtccagcgca gccaccaagc ccgcagatac cgacccgccc
     2041 aggcatcaaa agacgccaga tcgaacagcc gccatccaac gagcatcccg acgaccgcgc
     2101 tggcggtgat gg
//
LOCUS       DATADN010000908         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1174882, whole genome shotgun sequence.
ACCESSION   DATADN010000908 DATADN010000000
VERSION     DATADN010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174882"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..530
                     /locus_tag="VHH53_11785"
     CDS             180..530
                     /locus_tag="VHH53_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX2290846.1"
                     /translation="MGVFDRLLRRERPGTKRAATSADVDHLEQWAASHRGVEAFVEPR
                     TAVTDTTVVLVAHDGEWTRRRVEGDAGVQRLAKRLQIPIYDVAQMGYPQRMRDYTARQ
                     RILREREQEREPKQ"
     gene            complement(535..>1731)
                     /locus_tag="VHH53_11790"
     CDS             complement(535..>1731)
                     /locus_tag="VHH53_11790"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX2290847.1"
                     /translation="AGAAAVIVTAVLAGRLELPVEVIATVPMAENMPSGTAYRSHNGD
                     GPLSRMDLSVPSASAASAQQQLARATAIAEAVAAARDLVNTPPNDLYPASFAERAVTL
                     AEQAGLRTEVLDEKALRREGYGGIVGVGGGSSRPPRLVRLHWVPTAAASTPRAKVALV
                     GKGITFDTGGISIKPAAKMDEMTMDMAGAAAVIVTAVLAGHLELPVEVIATVPMAENM
                     PSGTAYRPGDVLTMYGGKTVEVLNTDAEGRLILADALVRAAQDSPDYLIDTATLTGAQ
                     IVALGSRTPGIMGTPELRDRITAISQEVGENGWAMPLPDHLRPDLDSKLADIANVAGH
                     RWGGMLAAGIFLSEFVADGIPWAHLDIAGPAYNTGSPWGYTGKGGTGVPVRTLAAVLT
                     DIAERG"
BASE COUNT          277 a          586 c          616 g          252 t
ORIGIN      
        1 cacacaggca tcggtgacgt ggcgagcggg cgcctagccc atcttgtcac cacgcccgga
       61 gttccggcga cgtgggcgaa gccatctggc agcccggtac gaagcgggag caaacggacc
      121 ggcacggtcg ttgaactggc aggattgccg ggaggatcgg cgttggagga ggtgccgacg
      181 tgggtgtgtt cgatcgactc ctgcggcggg agcggccagg aaccaagcgc gcggcgacca
      241 gcgccgacgt cgaccacctt gagcagtggg cagcgtcaca ccgtggagtg gaggcgttcg
      301 tcgagccccg caccgcggtc accgacacca ccgtggtgct ggttgcgcac gacggcgagt
      361 ggacccggcg cagggtcgaa ggcgacgcag gcgtccagcg actggcaaag cggctgcaga
      421 tcccgattta cgacgttgcc cagatgggct acccgcaacg gatgcgggac tacacagccc
      481 gccagcgcat cctgcgcgag cgcgagcagg aacgcgagcc gaagcagtag atattcagcc
      541 ccgttcggcg atgtcagtca ggacagcggc cagggtacgc accggcaccc cggtgccgcc
      601 cttgccggtg taaccccacg gagagccggt gttgtaggcc ggcccggcga tgtccaggtg
      661 tgcccacgga atgccgtcgg cgacgaactc ggacaggaat atcccggcgg cgagcatgcc
      721 accccagcgg tgaccggcca cgttggcgat gtccgcgagc ttggagtcca ggtcagggcg
      781 cagatggtcg ggcagcggca tggcccaccc gttctccccg acctcctgcg agatcgcagt
      841 gatccggtcc cggagctccg gagtgcccat gatccccggc gtacggctac ccaatgccac
      901 tatctgggcg ccggtgagcg tggcggtatc gatgaggtag tcgggggagt cctgcgctgc
      961 gcggaccagc gcatcggcca ggatcagccg gccctcggcg tcggtgttga gcacctcgac
     1021 ggtcttgccg ccgtacatcg tgaggacgtc cccgggccgg taggcggtgc ccgagggcat
     1081 attctcggcc atcggtacgg tcgcgatcac ctccaccggc agctcgaggt ggccagcgag
     1141 caccgcggtg acgatcacgg cggcggcacc cgccatgtcc atggtcatct catccatctt
     1201 cgctgccggc ttgatcgaaa tcccgccggt gtcgaaggtg atccccttgc cgaccagggc
     1261 gaccttcgcc ctcggcgtgg acgcggctgc ggtaggcacc cagtgcagcc ggaccagccg
     1321 gggcgggcgg gacgagccac cgccgacgcc cacgatgccg ccgtaacctt cgcgccgcag
     1381 cgctttctcg tcgagcacct cggtgcgtag cccggcctgc tcggccagtg tcactgcacg
     1441 ctcagcgaac gaggctggat agaggtcgtt gggtggcgtg ttcaccagat cacgtgcggc
     1501 cgcgaccgcc tcggcgatag cggtcgcccg ggccagctgc tgctgtgcgc tagcggcact
     1561 ggccgagggc accgacaggt ccatccggct cagcggcccg tccccgttgt gggaccggta
     1621 cgcggtgccc gagggcatgt tctcggccat cggtacggtg gcgatcacct ccaccggcag
     1681 ctcgaggcgg ccagcgagca ccgcggtgac gatcacggcg gcggcgcccg c
//
LOCUS       DATADN010000909         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1474405, whole genome shotgun sequence.
ACCESSION   DATADN010000909 DATADN010000000
VERSION     DATADN010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474405"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..513)
                     /locus_tag="VHH53_11795"
                     /pseudo
     CDS             complement(295..513)
                     /locus_tag="VHH53_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612020.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(556..855)
                     /locus_tag="VHH53_11800"
     CDS             complement(556..855)
                     /locus_tag="VHH53_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HEX2290848.1"
                     /translation="MTTTDTHNRVERACAQLHHDGQHITFTAIAATTGLGRTTLYRDP
                     TLRAIIDQHRHHATTSGTLTALTDVIATLRTALETIATRVRRHEEQLQRLTAHKD"
     gene            complement(852..>2676)
                     /locus_tag="VHH53_11805"
     CDS             complement(852..>2676)
                     /locus_tag="VHH53_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2290849.1"
                     /translation="NTSYWQAARVFFRRWPDPRRWANEPLQTRLSAGSATRPLITFLM
                     LHRVLQPGYDYLLERKLSSIWREIKDSPLGPDLDRFMTAAADLGFTERVRFATGSQVP
                     ARLLIQTRRHLDQLTVADLEKFSAACHDRHQHTGKGHKHFLAAVSNAQRVLFHLGIVD
                     QLPRSGGPVPFAERLAEVRLPIRKTMIAYLERKRATCQPKTVSGIATRLKHFGVFLAH
                     VDPDLDSIANLDRRTHIEPYLTSLVEAVNSKNDEFITVADRSRRVLALTGFLTDITEW
                     GWPQAPPRKLVFRDDNPKLPQVLPRYLPVDVDRRLTQALVEHPDNELAAAALRLQRSC
                     GLRIGELLDLELDCVHELPGHGSWLKVPLGKLDTERMVPLDAEILTLIDQITELRSHG
                     RPMPHPRYRRPAQFLFTHHGRRLSQNAVRAELDRATHTAGLDHVTPHQLRHTYATALV
                     NAGVSLQALIALLGHMSAEMSLRYGRLFDTTVRAEYERVLDLAKQQAQTPTPSRRALP
                     LADITGGADWKDTPLLKSRLASGFCLRAPAQGACAYANICEHCPNFHAAPSSLPILAA
                     QRVDAKALAADAENRGWITEAQRHRRLITQLDTLINEAHTG"
BASE COUNT          364 a          688 c         1066 g          558 t
ORIGIN      
        1 caagcaagcc ccgggggcgt gcgggttctc tgttgagggc gctggtgggc tgccgtgagc
       61 ggcgctcgcc gttggcagtg agcgtgtaca aggtggagcc agttgccgtt gttgtccggt
      121 gcgccgcgtt gctccgtgta gccaatccat tgtctggtga tggctttgcg ggtagcgcgc
      181 agccattgtt cccattcgtg gggggattgt ggctgctggt cggcgtgcag caggtagtcc
      241 ccgcagtcag gatgggccgc ctcgttcttt ccggacagct gactaattgc tatgtcatgc
      301 tgcggtctgc cggttcaggt actcgtcgtg gacctcttgc ggggtctggt agccaagtgc
      361 cgagtggaga cgtttagtat tgtagcgaaa ttctatatac cgggcaacat cctttcgggc
      421 gtgttcgcgg gtggggtaga cggtgcggtt gacccgttcg actttcacgg cggcgttgaa
      481 ggattcggcg agggcattgt cgaaacatta tccggctaat gatgttggtt tctggcgttt
      541 gaccggttaa cgctttcagt ctttgtgggc ggtgaggcgt tggagttgtt cttcgtggcg
      601 gcgtactcgg gtggcgatgg tctccagggc ggtgcgcagg gtggcgatca cgtcggtgag
      661 cgcggtgagg gtgccgctgg tggtggcgtg gtggcggtgt tggtcgatga tcgcgcgcag
      721 ggtggggtcg cggtagaggg tggtgcggcc caggccggtg gtggcggcga tggcggtgaa
      781 ggtgatgtgc tggccgtcgt ggtggagctg ggcgcaggcg cgctcgacgc ggttgtgggt
      841 gtcggtggtg gtcatccggt gtgggcctcg ttgatcaggg tgtcgagttg ggtgatgagt
      901 cggcggtgtc gttgggcttc ggtgatccag ccgcggtttt cggcgtcggc ggcgagtgct
      961 ttggcatcga cgcgttgggc ggcgaggatc ggcagcgagc tgggggcggc gtggaagttg
     1021 gggcagtgtt cgcagatgtt ggcgtaggcg caggctcctt gggccggtgc gcggaggcag
     1081 aatccgctgg ccagtcggga tttgagcagt ggggtgtctt tccagtcggc gccgccggtg
     1141 atgtcggcca acggcagggc gcgccggctg ggagttgggg tttgtgcttg ctgtttggcc
     1201 aggtcaagga ctcgttcgta ttcggcgcgg acggtggtgt cgaacagtcg tccgtagcgc
     1261 aggctcatct cggcggacat gtgccccagc agcgctatca gtgcctgcaa cgagactccg
     1321 gcgttgacca gtgcggtggc gtaggtgtgg cgcagctggt gcggagtgac gtggtcgagc
     1381 ccggcggtgt gggtggcccg gtcaagttcg gcgcggaccg cgttttggga tagccgtcgg
     1441 ccgtggtggg tgaacaggaa ctgggcgggt cgccggtagc gggggtgggg catcgggcgt
     1501 ccgtgggagc ggagctcggt gatctgatcg atcagtgtga ggatctcggc atcgagtgga
     1561 accatccgct cggtgtcgag cttgccgagc gggactttca gccagctgcc gtgaccgggt
     1621 agttcatgca cacagtccag ctcgaggtcg agcagttccc cgatgcgcag cccgcaggat
     1681 cgctgtagcc gcaaagcggc cgcggccagc tcgttgtcgg ggtgttcgac cagcgcctgg
     1741 gtgagccggc ggtcgacatc gacgggcagg tagcgcggca gcacttgcgg gagtttgggg
     1801 ttgtcatcgc ggaagaccag cttgcgtggg ggtgcttgcg gccagcccca ctcggtgatg
     1861 tcggtcaaaa acccggtcag cgcgagcacc cgccgggagc ggtcggcgac ggtgatgaac
     1921 tcgtcgtttt tggagttgac cgcctcgacc agcgaggtca ggtagggctc gatgtgggtg
     1981 cggcgatcga ggttggcgat cgagtccagg tcggggtcga cgtgggcgag gaacaccccg
     2041 aagtgcttga gccgcgtcgc gatccccgag accgtcttgg gttggcaggt cgcgcgtttg
     2101 cgttccagat aggcgatcat cgtcttgcgg atcggtaggc gcacctcggc caggcgttcg
     2161 gcgaacggga ccggcccacc cgagcggggc agttgatcga cgatcccgag gtggaacagc
     2221 actcgttggg cgttgctgac cgccgcgagg aaatgcttgt ggcccttgcc ggtatgctgg
     2281 tggcggtcgt ggcaggccgc gctgaacttc tccaggtcgg cgacggtgag ctggtccagg
     2341 tgccgtctgg tctggatgag cagccgggcc ggcacctgcg agccggtggc gaagcggacc
     2401 cgctcggtga accccaggtc cgcggccgcg gtcatgaacc ggtcgaggtc cgggcccaat
     2461 ggggagtctt tgatctctcg ccagatgctg gagagtttgc gttccagcag atagtcatag
     2521 ccgggttgca gcacccggtg cagcatcaag aacgtgatga gcggccgggt ggccgatcca
     2581 gcggacaacc gggtttgcag cggttcgttg gcccagcgcc gcgggtctgg ccagcgccgg
     2641 aagaacaccc gagccgcctg ccagtagctg gtgttg
//
LOCUS       DATADN010000910         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_96342, whole genome shotgun sequence.
ACCESSION   DATADN010000910 DATADN010000000
VERSION     DATADN010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96342"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VHH53_11810"
     CDS             complement(<1..235)
                     /locus_tag="VHH53_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290850.1"
                     /translation="MTTMFAEDEVEDRMPAPTIPSRGGEGGDWHVIEELPAADPDLRA
                     QDWPSGRDGGEDSAGSGGQSLGHERDAAAGAEPD"
     gene            911..1288
                     /locus_tag="VHH53_11815"
     CDS             911..1288
                     /locus_tag="VHH53_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290851.1"
                     /translation="MRAQAARQWCAWEDTHMSLAPEWSPGLSHRDPEFQLTFARLVTH
                     YWSHGCFLAEGEILANMHRLADISAVLIHGRYDISGPLDTAWRLHRSWPNSKLVMLDD
                     AGHGGGSFTSELVGALDSFRTVR"
     gene            complement(1375..2121)
                     /locus_tag="VHH53_11820"
     CDS             complement(1375..2121)
                     /locus_tag="VHH53_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2290852.1"
                     /translation="MDIPRSFTIRESSHRIHNPLTSDKLATLGQALALRSGTRMLDLA
                     CGTGEMLCTWARDHHVTGTGVDISTVFTAAARARAIELGVADKITFVHGDASGYVADE
                     PIDVASCIGATWIGAGMPGTIELLRRGLRPEGIMLIGEPYWRRDPPDQATIDACEAST
                     KDDFLPLPELVERFQELGWDLVEMVLADQDSWDRYIAAQWLNIRRWLDDNPDDDLADE
                     MRAELTTSPLRHTKYQREYLGWGVFALMKR"
     gene            2332..>2577
                     /locus_tag="VHH53_11825"
     CDS             2332..>2577
                     /locus_tag="VHH53_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290853.1"
                     /translation="MSTKALETLLRKLPDLFHLMLVGLVEFCARLPQSPRRVLGDVSA
                     VHNTDLSTSPLVGDVCLGHDVVRRDSHQQLTGHGVAAT"
BASE COUNT          411 a          866 c          836 g          464 t
ORIGIN      
        1 cgtcaggctc cgcgcccgcg gcggcgtccc gctcgtgccc cagcgactgc ccaccagacc
       61 cagccgagtc ctctccgccg tctcgcccgc tgggccaatc ctgcgcccgc aggtcaggat
      121 cggccgcggg aagctcctcg atcacgtgcc aatcccctcc ctcgccgccg cgactgggga
      181 tggtaggcgc tggcatgcgg tcttcaacct cgtcttcggc aaacatggtc gtcaccgctc
      241 cactcctaca ccgggcctga cgaccgggcg cccgcaccga cgacacccag acgccgccga
      301 ggttcaaccg atggccgcct cgaaggcggc tagcggcggc actgctctat taagacgact
      361 cttcacttaa gacacacctg gcgggtgatc cgcgtccacg cgagctgagc ctgcggcgag
      421 gtcgttggtc ggctcggtgc gagggtaacg agcaaggcag ggcttcgtgg acgccccggc
      481 ctcgcggcca gatcgtgtct cgggtcgact ccgggcgcat gtgccgatga caggaagcac
      541 tgcggcctcg ccgcatcagt tccgggtcga ttgcctgggc tcgcgagagt tgggtcgtgg
      601 agactgtcct gatggctgag cgttacccgg cggcggagcc ctacaagtcc ggcatgctga
      661 ccgtgacaga cggacaccac ctgtactggg aatgtgcgga gcccccgacg gaaccgcagc
      721 ggtctacctg cacggtggtc ccggaagcgg gtgcaccccc ggcgcacgcc gcaattcgat
      781 ccccgctcct accgtgctgt gctgttcgat cagcgcgggt gcgcgcgcag ccgaccttcc
      841 gccggcgacg aggatgccga tctgtcgacg aacaccacgg cgccgactgc tggcggaccc
      901 ggatccgcag atgcgtgccc aggcggcgcg gcagtggtgc gcgtgggaag acacgcacat
      961 gtcgctggcg ccggagtggt ctcctggcct cagccaccgg gatccggagt tccagctgac
     1021 cttcgcccgg ctggtcacgc actactggag ccacggctgt ttcctcgcag aaggagagat
     1081 cctggccaac atgcaccgcc tggccgacat ctccgccgtg ctcattcacg gccgctacga
     1141 catctccggc ccgctagaca ctgcatggcg cctgcatcgc tcctggccga acagcaagct
     1201 tgtcatgctc gatgacgccg gccatggcgg aggcagcttc accagcgaac ttgtcggtgc
     1261 cctcgactcc ttccgcaccg tgcgctagcg cgtccactgg tgagacgacg ggctaccgac
     1321 atgaacgcgc tgccatgccg ctgaccgcat gtccggtcgt cgtggtgcgc cgggttagcg
     1381 tttcatgagg gcgaagacgc cccacccgag gtattcgcgc tggtatttgg tgtggcgcag
     1441 cggggaggta gtcaactccg cccgcatctc gtcggctagg tcgtcgtcgg gattgtcgtc
     1501 tagccagcgg cggatgttga gccattgcgc cgctatgtac ctatcccagc tgtcctggtc
     1561 ggccaggacc atctcgacga ggtcccagcc cagttcctga aaccgctcga ccagctccgg
     1621 caggggcagg aaatcgtcct tggtgctggc ctcgcaggcg tcgatggtgg cctgatcggg
     1681 gggatcgcgt cgccagtacg gctcaccgat caacatgatg ccctcggggc gcaggccgcg
     1741 ccggaggagc tcgatggtgc cgggcatgcc agcaccgatc caggttgcgc cgatacacga
     1801 ggcgacgtcg attggttcat cggcgacgta gcccgatgca tccccgtgca cgaaggtgat
     1861 cttgtctgcc acacctagct cgatggctcg cgcgcgtgcg gcggcggtga acacggtgct
     1921 gatgtcgacc ccggtaccgg ttacgtgatg gtcgcgtgcc caggtacaga gcatctcgcc
     1981 cgttccgcag gcgaggtcga gcatgcgcgt ccccgatcgc aaggcaagcg cctggcccaa
     2041 ggtcgccaac ttgtcgctag tgagcgggtt gtggatgcgg tgactgctct cgcggatggt
     2101 gaaactacgt ggaatatcca ctcctggagg tccttacgtt cacggtattc atgacggcca
     2161 ggtcctgtgg tcggtcggca ccgactggat cacctggaca gccacccaat ccacctcatt
     2221 aagtggaagg ccacgctgac gcgggcgacg ctaaaggcat ccagccgcca tgtccacggg
     2281 ttattggctt cctgcccgac gtccgctctt gccgcgaggc gggcgttcgc aatgagcacg
     2341 aaagcgctgg agacgctact acgaaagctg cccgacctct ttcatctgat gctggtcgga
     2401 ctggtcgagt tctgcgccag gttgccgcag tcgccgcggc gtgtgcttgg cgacgtctcg
     2461 gcagtccata acacggactt gtcgacttcg cctttagtcg gcgatgtctg cttgggccac
     2521 gacgtggttc ggcgtgattc gcaccagcag ctcactgggc atggcgttgc ggcgacc
//
LOCUS       DATADN010000911         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1306043, whole genome shotgun sequence.
ACCESSION   DATADN010000911 DATADN010000000
VERSION     DATADN010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306043"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VHH53_11830"
     CDS             complement(<1..217)
                     /locus_tag="VHH53_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2290854.1"
                     /translation="MPAARSGGLARPRVFPARPGQAAMIIAAFTGVLYLTEAVDMVIG
                     GALNDDGIRPRQLDGLDGVLWAPLLHGD"
     gene            complement(296..1246)
                     /locus_tag="VHH53_11835"
     CDS             complement(296..1246)
                     /locus_tag="VHH53_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HEX2290855.1"
                     /translation="MARYDSLLDALGDTPLVGLPHLSPAPDVRLWAKLEDRNPTGSVK
                     DRPALAMIEDAEQSGRLTEGCTLLEPTSGNTGISLAMAAKLKGYRLVCVMPENTSIER
                     RQVLEAYGAEIIFSPAAGGSNQAVARAKELAAAHSDWVMLYQYGNPANAAAHYRTTGP
                     EILRDLPTVTHFVAGLGTTGTLVGVGRYLREHKPDVQIIAAEPRYGELVYGLRNLDEG
                     FVPELYDPEVLTGRFSVGSYDALRRTRELLECEGIFAGISTGGILHAALAVAEKAAGT
                     GDAADIVLVVCDAGWKYLSTGAYSGDLEEAAARLDGQLWA"
     gene            complement(1286..1585)
                     /locus_tag="VHH53_11840"
     CDS             complement(1286..1585)
                     /locus_tag="VHH53_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX2290856.1"
                     /translation="MAVTVSVPTILRTHTGGEKSVEASGSNVGEVIEDLESRHSGIKA
                     RLVKEGSLHRFVNVYVNDEDVRFAGGLAAAVKDGDNVTILPAVAGGAARECVTVS"
     gene            complement(1668..2123)
                     /locus_tag="VHH53_11845"
     CDS             complement(1668..2123)
                     /locus_tag="VHH53_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX2290857.1"
                     /translation="MLTIRRDLVDAMVLHARRDHPDEACGVLAGPVGSDRPERFIEMA
                     NAERSPTFYRFDSAEQLQVWRAMDAADEEPVVIYHSHTATEAYPSRTDISYASEPDAH
                     YVLVSTRDPDTHELRSYRIIDGEVSEEPIEVVESYMFSHTGADDVPDAH"
     gene            complement(2133..>2395)
                     /locus_tag="VHH53_11850"
     CDS             complement(2133..>2395)
                     /locus_tag="VHH53_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEX2290858.1"
                     /translation="AVKDGVAAVVLDTCPARGGRVRLTDEQAQAWLSALNDVRLALGT
                     VLDVTEDMPDELPPDDPRAPHLGVYHWLTWVQDSLIHAVSDT"
BASE COUNT          399 a          832 c          783 g          381 t
ORIGIN      
        1 agtcgccgtg cagcagcggg gcccacagaa cgccgtccag cccgtcgagc tgccggggcc
       61 ggatcccgtc gtcgttgagg gcaccgccaa tcaccatgtc caccgcttcg gtgagataca
      121 gcacgccggt gaacgcggca atgatcatgg cggcctgccc gggccgagcc ggaaacaccc
      181 gcggacgggc cagacctccg gagcgcgcgg caggcacgtc acccgaccct accctcgcac
      241 cctgagagaa ccctgtgcct ccccgcgttc ccccgctgaa tgccgattgg caaagttacg
      301 cccagagctg gccgtcgagc cgggcagcgg cctcctcgag gtcgcctgag taagcgccgg
      361 tggacaggta cttccacccg gcgtcgcaga ccacgagcac gatgtccgcg gcgtcaccag
      421 tgcccgccgc cttctccgcg acggccagcg cggcgtgcag gatcccgccg gtggagatac
      481 cggcgaagat gccctcgcat tccagcagct cgcgggtgcg gcgcagcgcg tcgtaggagc
      541 ccacggagaa ccggccggtg agcacttccg ggtcgtagag ctcgggcacg aagccctcgt
      601 cgaggttgcg caggccatag accagctccc cgtagcgcgg ctccgcagcg atgatctgta
      661 cgtcgggctt gtgctcgcgc aggtagcgcc ctaccccgac cagggtgcca gtagtgccga
      721 gcccggcgac aaaatgggtc acggtgggca ggtcgcgcag gatctccggg ccggtggtgc
      781 ggtagtgcgc cgcagcgttg gccggattgc cgtactggta gagcatgacc cagtcggagt
      841 gcgcagcagc cagctccttg gcccgggcga ccgcctggtt ggagccaccc gcagccggcg
      901 agaagatgat ctccgcgccg taagcctcaa gcacctgccg ccgctcgatc gaggtgttct
      961 ccggcatcac gcacaccagc cggtaaccct tgagcttcgc tgccatggcc agcgagattc
     1021 cggtgttgcc cgacgtcggt tccaggaggg tgcagccctc ggtgagccgc ccggactgct
     1081 cggcgtcttc gatcatcgcc agcgccgggc ggtctttcac cgagccggtg gggttgcgat
     1141 cctccagctt ggcccacagc cgcacgtcgg gtgccggtga caggtggggc aaccccacca
     1201 gcggggtatc acccaacgcg tcgagcaacg agtcatagcg ggccatggcg ctcccttcgg
     1261 tcgcgctcgt gagtggcgtt cagtgctatg acaccgttac gcactcacga gcggccccgc
     1321 ccgccactgc cgggaggatg gtgacgttgt cgccgtcctt caccgcagct gccaagcctc
     1381 cggcgaagcg cacgtcctca tcgttgacgt agacattgac gaagcggtgc aaggatcctt
     1441 ccttcaccag ccgggctttg atccccgagt gccggctctc cagatcctcg atcacctcgc
     1501 cgacgttgga ccccgacgcc tccaccgatt tctcgccccc tgtgtgggtg cgcaagatgg
     1561 tgggtacgga gacagtgacg gccattgtcg aatcctccta taggtgcggg cgactcacca
     1621 gttcctctac gcactaccag cctcggtcgg tgagggcggc gcgctggtca gtgtgcgtca
     1681 gggacgtcgt cagcgccggt atgggagaac atgtacgact ccaccacctc gatcggctcc
     1741 tcgctgacct cgccatcgat gatgcggtag gagcgcaact cgtgagtgtc cggatcccta
     1801 gtggatacca gcacgtaatg cgcatcgggc tccgacgcat aggaaatgtc agtgcgcgac
     1861 gggtaggcct cggtggcggt atgcgaatgg tagatcacca ccggctcctc gtcggcggca
     1921 tccatcgccc gccacacctg cagctgctcg gccgagtcga accggtaaaa ggtcggtgag
     1981 cgctcagcgt tggccatctc gatgaatcgc tccgggcggt cagagcccac cgggcccgcg
     2041 aggacaccgc acgcctcatc cgggtgatcc cggcgggcat gcagaaccat tgcgtcgacg
     2101 aggtcacggc ggatcgtcag cacgccttca tcctacgtgt ccgagacggc gtggatcagg
     2161 gagtcttgca cccaggtcag ccagtggtac acgcccaggt gcggcgcgcg gggatcgtcg
     2221 ggcggaagct catccggcat gtcctcggtg acgtcgagca cggtacccaa cgccaagcgg
     2281 acgtcgttga gcgcggacag ccacgcctgc gcctgctcgt cggtcagccg cacccggcca
     2341 ccccgagccg ggcaggtgtc cagcacgaca gcagccaccc cgtccttgac ggcca
//
LOCUS       DATADN010000912         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1474594, whole genome shotgun sequence.
ACCESSION   DATADN010000912 DATADN010000000
VERSION     DATADN010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474594"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VHH53_11855"
     CDS             <1..345
                     /locus_tag="VHH53_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX2290859.1"
                     /translation="VVIADNVCTNVDVQCLIATIDDRRAASAPPGQTTITFVGNTCEV
                     NGSQAVLLRNFSDVIIRDNRFSGPGDRAVQLSGESTNCTVVGNTVTGRMRPFEIDRQS
                     EPGFEEGGNTSG"
     gene            850..1440
                     /locus_tag="VHH53_11860"
     CDS             850..1440
                     /locus_tag="VHH53_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX2290860.1"
                     /translation="MTFTPVACGDDESSEESQAPAPAPAGPPNTSTLSGVDTFVAPSP
                     GAEAVTYNPALVPEGASILASIMPACFGYTETRANLSAAGLLPNHAYVVRAHTQACGA
                     TAEDAGPPYQNRVDPAATPQGASTNPEYANPRNELWLDIRTDSDGSASSGASVPFTFT
                     DRAPASVVVYDAASGQAGQLGAPIACLNLSSTPRSG"
BASE COUNT          286 a          555 c          481 g          279 t
ORIGIN      
        1 gtcgtcatcg ccgacaacgt ctgcacgaac gtcgacgtcc agtgcttgat cgcgaccatc
       61 gacgatcgcc gtgccgctag cgcaccgccg ggacagacca cgatcacctt cgtaggcaac
      121 acctgtgaag tcaacggttc ccaggcagtc ctactgcgga atttctccga cgtgatcatt
      181 cgtgacaatc ggttctccgg tcccggcgac cgtgcagtac agctcagcgg ggagtcgacc
      241 aactgcaccg tggtcggcaa caccgtgacc ggtcgcatgc gcccgttcga gatcgaccgg
      301 cagtccgagc cagggttcga ggagggcggc aacacctcgg gctgatctac gccgcttcgg
      361 tgagatcgag gtaaccgcgt tcgacccgcc gccctcggcg gacctcgggt ccggttgaca
      421 atggcatccc agtcgacggt tcccagcctg aaagcaaccc tgtggccgcg acggctacct
      481 gtgtttcctc ggccgcgcca gccctgatta agggcccgac gctgccctcc ggatagcacc
      541 cacctccgtc ccacggaact gggtcgtcgt agccaggacg gtagccattc gtcgaggtta
      601 ccggcgttcc tgtcgcgcgg ggaaagttgc gcggattaag ggagccgccc aggtgctgcg
      661 tgtcccgatc ccggtttcga gatggcctcg ccaagtgtag atcaacatgt catcatatat
      721 gccatgggga gactattctt gggtgagaag catcacatga cgtcctcgta tgccggatca
      781 ggcgagcagt cggtggctat tcggcgaccg atgtgtcgcc ggctggccgt cgctgtcgtc
      841 gcaaccgcca tgactttcac accggttgcc tgcggcgacg acgagtccag cgaagaatcc
      901 caggcgcctg cgccggcacc cgccggaccc ccgaatacct cgaccctgtc cggcgtcgac
      961 actttcgtcg cgcccagccc gggcgccgaa gccgtcacct ataatccggc cctggtgccg
     1021 gagggcgcat cgatcctggc cagcatcatg ccggcgtgtt tcggctacac cgaaaccagg
     1081 gcgaacttgt cggccgcggg gctgctgcct aaccacgcct atgtcgtacg cgcgcatacc
     1141 caggcctgcg gcgccacagc cgaggacgca ggaccgccct accagaatcg cgttgacccc
     1201 gccgcgacac cccaaggggc ctcgaccaat ccggagtacg ccaatccgcg taacgagctc
     1261 tggctagata tacgcaccga ttcggatgga tcagccagct ccggcgcgtc cgtgcccttc
     1321 accttcaccg accgggcacc cgcatcggtc gtggtgtacg acgcagcttc cggccaggca
     1381 ggtcaactgg gcgctccgat cgcctgcctc aacctgtcca gcactccccg gtccggttaa
     1441 cgaccggcgc ggggtcacgt cgatttagcc agcagcacgt cacgcggcag cttgccggtg
     1501 cgagtagccg gtgctgcgcc gatccgtggg acccagccgc ccttgacgac cgagtaatat
     1561 cgagatgggc acggtagttg agcccatcag actcaatctt c
//
LOCUS       DATADN010000913         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1474664, whole genome shotgun sequence.
ACCESSION   DATADN010000913 DATADN010000000
VERSION     DATADN010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474664"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            651..797
                     /locus_tag="VHH53_11865"
     CDS             651..797
                     /locus_tag="VHH53_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290861.1"
                     /translation="MSDALSFTEIDGQHVELLPATVLSLFAAGGFGDDGGSADGGAGG
                     ACYS"
     gene            1015..2412
                     /locus_tag="VHH53_11870"
     CDS             1015..2412
                     /locus_tag="VHH53_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2290862.1"
                     /translation="MIQIPTRHLEALRTVLAGKVITPDDADYDTARIQWNNNIDRRPV
                     AIARCVSPADIAAALAFGREQSLEISVRGGGHAFSGAGVCDGGLMIDLSAMRHVSVCA
                     ATVVARVGGGATVAELDAATQTHGLAVPAGVISHTGIGGLTLGGGIGWLTHKSGLSID
                     NLVSVDVVLADGACVHASADEHSDLFWAVRGGGGNFGVVTTFEFQLQPVGPEVHLGLF
                     FWGMDDGEQVLRLARDLIPALPHNAGAQIAIGLNAPPAPFVPQQHHFVPGYLLIVVGF
                     STAEEHAQLVAPIRETLPPLFELVTPIPYVALQRTLDETAPWGLLGYQEVIYLDELTD
                     DAIAVIVERMPAKSSPMSLCVLYRLDGAYSAIGDDETAFGGGRAPRYVINIAGIGPTP
                     ELLTADRAWVRSVWNALRPFAQHSGGYVNSMTDDDESRVRASYGPAKYERLALIKARY
                     DPDNIFHLNANIKPA"
BASE COUNT          477 a          852 c          813 g          485 t
ORIGIN      
        1 cgacagcacg actactcatc ggttcactgc tgcggcagtc cgagaccgtc tgcgtcgcag
       61 tctgccgatg gcaccgagtt cccacggcgc tgcagttcag cgacgtcagg cgcagccagc
      121 tgcgggcttt ggtccgagaa cccttgactc aacgagtcgc tggccacctt tggtaaggca
      181 tcgccagcgg tgatgacagc gattaccgca accaccagat tcgtggcgcg tcgccggtct
      241 gctgaagcag ccgacgccct attgggccaa caacggcgat ttcgaccgct acaccgagtc
      301 attgacagcc aggcgctgcc gtgcaaaagg cgcgagatgg ctagcgactg attactcgcc
      361 gagcaaccgc atggacactg tatgtcaaaa agaataaagt acgccggcac gcccagtcgg
      421 acgtacaccg tagctaggta ccgggcgtcg gacacctacg tccgtgtcac cgcggactac
      481 gtattcgaaa agcaactcca tggctacgta gtctacctgc agatttacgt gcaactactg
      541 attgcccgcc acggccccac tcggcaatgt atgcattgcc gaatcggtat gctaccgatg
      601 aagcgctttt ccgccggtac cgccagggtt acattcccta ggaggattcg atgtctgacg
      661 cactgagctt taccgagatc gatggacagc acgtcgagct gttgccggct acggtcctgt
      721 ccctgttcgc cgccggcggc ttcggtgacg acggcggcag tgccgatggc ggtgctggcg
      781 gcgcctgcta cagctgacag gtcgcggcgg tgccgctggt cctcgcgtgc ggtgccgctg
      841 gcgagggagg ggtggtcaca gtgcggctgc ccctcccctc atctttggtt tcatgcagta
      901 acagcattac gtgatcgtca atgtgcgatt gttgaggatc ccgcagcatt aacgttgtac
      961 ttaagtcgca ccaggcgcac gccgggtgct cgcccgaggc ctgggagcgg ttccatgatt
     1021 caaatcccta ctcgccacct ggaggcgctc cgcaccgtgc tggccggcaa ggtgatcacg
     1081 ccggacgacg ctgactacga caccgcacgc atacaatgga acaacaacat cgatcgtcgt
     1141 ccggtggcga tcgcccgctg tgtatcccca gccgacatcg cggcagcgct cgccttcggc
     1201 cgagagcaat cgttggagat ctcggtgcgc ggcggtggcc atgcgttctc cggggccggc
     1261 gtctgtgatg gcgggttgat gatcgatctc agcgcgatgc gccacgtctc ggtctgtgcc
     1321 gccactgttg tggccagggt gggaggcggc gccaccgtgg cggagctgga tgcggcaacc
     1381 cagacgcatg gcttggcggt gcccgccgga gtgatcagcc acaccgggat cggaggactg
     1441 acgcttggtg gcggtatcgg atggttgacc cacaaatccg gtttgagcat cgacaacctg
     1501 gtatcggtcg acgtcgtgct cgccgacggc gcatgcgtgc atgcgtcggc cgacgagcac
     1561 tccgacctgt tctgggcggt ccgcggtggg ggcggcaact tcggcgtcgt cacgaccttc
     1621 gagttccagc tgcagccggt cggacctgaa gtgcatctgg gcctgttttt ttggggaatg
     1681 gacgacggcg agcaggtgct gcgccttgcg cgcgatctca tcccagccct tcctcacaac
     1741 gccggcgccc agatcgcgat cggactcaac gctcccccgg caccgttcgt gccccagcag
     1801 caccacttcg tcccgggcta cctgctgatc gtggtcggat tctccaccgc cgaagagcat
     1861 gcccagcttg tcgcgccgat tcgggagacg ctaccgccgc tgttcgagtt ggtcactccg
     1921 atcccgtacg tagcattaca acggacactc gacgagaccg ctccatgggg actcctcggc
     1981 tatcaagaag tgatctatct cgacgagctc accgacgacg cgattgcggt gatcgtggaa
     2041 cggatgcctg ccaaaagctc gccgatgagc ttgtgcgtgc tgtaccggct ggatggagca
     2101 tattcggcga tcggcgatga cgagaccgcc ttcggcggtg ggcgagcacc acgctacgtc
     2161 atcaacattg ccggaatcgg cccgacgccc gagctgctga ccgccgaccg agcatgggtt
     2221 aggtcggtat ggaatgcgct gcggccgttt gcccaacact ccgggggtta cgtgaactcc
     2281 atgaccgacg atgacgagag ccgggtgcgc gcatcctacg gcccggccaa gtacgagcgg
     2341 ttggccctga tcaaggccag gtacgacccg gacaacattt tccacctcaa cgccaacatc
     2401 aaacccgcct gagtcaacac catcgcgaac ctcgaccgtc cgggttcgcc cgcgccggcc
     2461 agatcacgcc aggcgctatc gtcatcgacg tgagccccgc cccggctgcg ccgacccccg
     2521 cacccatcgg tgcgctggga gtgcccgaga ccgcacgtgc cttcctgctc gacctggacg
     2581 gcgtacttac ccaaacagcg aagcaacatg cggcggcctg gaagctg
//
LOCUS       DATADN010000914         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_714991, whole genome shotgun sequence.
ACCESSION   DATADN010000914 DATADN010000000
VERSION     DATADN010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714991"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VHH53_11875"
     CDS             complement(<1..271)
                     /locus_tag="VHH53_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2290863.1"
                     /translation="MQPYPDPVDTATVREFTQAEGAVRFAPVVVIIAAYDEEEGIGAV
                     LDGVPSASCGLPVDTLVIVDGCTDGTAEVARRHGARVCQVTINRGQ"
     gene            complement(286..1755)
                     /locus_tag="VHH53_11880"
     CDS             complement(286..1755)
                     /locus_tag="VHH53_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290864.1"
                     /translation="MTDLLAPAHVGAPKPGAALAFRTRRWLRGHWTLLVLLAAGAALR
                     VITVLAYQPAILYIDSFSYLNNIDDLRPNGLRPIGYHLILTIVLPLGGLRAVVIVQHL
                     LGLGIAVAIYLLLLRHGARRWLAALATAPVLLDAYQLQIEHNIMSDVWFQALLVAIIW
                     VLTWRGMPSPWHAAGAGALIGIAIIVRLVGITMLVPALAYLVVAGSLWCSRTGWRQIG
                     LRAAAMAAAFAVLLVGYAGYYRVSTGGWGISGGSDRVLYGRAAVIADCQRIPAGTWER
                     AICPVEPRDQRFGADYYAHHAASPARSVTLPPAESMDAIQASFARMVFANQPLDLTMA
                     VGKDFLKGFRPVRLDAVHDVPVDRWYFQTSYPYWWNPQQVAEMVRQFGDVPISVDAEL
                     ATVLRSYQLTVGYVPGPLLGLALAAGLLGGFGAGNARRSGIRAASLLVAGLGITVLVT
                     AAAFEFSWRYQLPGLVLLPMAGVLGITASLDRCAGQAPG"
     gene            complement(1752..>2671)
                     /locus_tag="VHH53_11885"
     CDS             complement(1752..>2671)
                     /locus_tag="VHH53_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2290865.1"
                     /translation="GFVGRAAVAAFIGRGNPVTVVDCRAHPDPAVHSVVGDLTDPGLI
                     AEALDGGDVAGVVHLAALTSVLRSVQHPAATYQANVAVTQDLLEYCRQAGVRRFVMAS
                     TNAVVGDVGYATMDETAPLRPLTPYGATKAACEMLLHGYAGAYGMAASALRFTNIYGP
                     GMEHKDSFVPRMMRAALAGRGVQIYGDGLQRRDLVHVDDVCQAVVSAWDRRFTGTAII
                     GSGQSVTVLELLEAARMVTGAALPAEHVEAKPGEMPAVIVDICHAREQLGYWPSVSLA
                     DGLARVWKDFTVADTVISTVTTDACTPDR"
BASE COUNT          487 a          938 c          884 g          362 t
ORIGIN      
        1 cctgaccccg gttgatcgtc acctggcaga ctcgcgcgcc gtgccggcgg gccacctcgg
       61 cggtgccgtc ggtgcacccg tcgacgatca ccaaggtatc caccggcagg ccacagctcg
      121 ccgacggcac cccgtcgagc acggcaccga taccttcctc ctcatcgtag gccgcgatga
      181 tcactacaac cggggcgaac cgcaccgcgc cttcggcctg ggtgaactcg cgcaccgtgg
      241 cggtgtccac cggatcgggg taaggctgca tgggaggtct cagcttcagc cgggggcctg
      301 gcctgcgcag cggtccagtg aggcagtgat ccccaggacg ccggccatcg gtagcagtac
      361 cagcccgggc agctggtaac gccaggagaa ctcgaaagcc gctgcggtca ccagcacggt
      421 gatcccgagc ccggccacca gcaggctggc agcacggatc cccgaccggc gagcgttgcc
      481 cgccccgaaa ccgccgagca gtcccgcggc cagcgccaag ccaagcagcg gaccaggcac
      541 atacccgacg gtgagctggt aactccgcag gaccgtggcc agttcggcgt cgacactgat
      601 cgggacgtca ccgaactggc gcaccatctc ggcgacctgc tgcggattcc accagtaggg
      661 gtaggaggtc tggaaatacc accggtccac cggtacgtcg tgtacggcgt cgagacggac
      721 cgggcgaaag cccttcagga agtccttacc caccgccatg gtgagatcca ggggctggtt
      781 ggcaaacacc atgcgggcaa aagaggcctg gatcgcatcc atcgactcgg caggcggcag
      841 cgtgacgctc ctggccgggg acgcagcgtg atgagcgtag tagtcggcgc caaagcgctg
      901 gtcacgcggc tccaccgggc agatggcgcg ttcccacgtc ccagccggaa tccgctggca
      961 gtcggcgatg accgcggcgc ggccgtagag cacgcggtcg ctgccgccgc tgatgcccca
     1021 gccaccggtg gagacccggt agtagccggc gtatccgacg aggagcaccg caaaggccgc
     1081 ggccattgcc gcagctcgta gcccgatctg ccgccaaccc gtccggctgc accacaacga
     1141 cccggcgacg accaggtagg ccagcgccgg gaccagcatg gtgatgccca ccagccgcac
     1201 gatgatcgcg ataccgatca gggcgcctgc cccggcggcg tgccacgggc tgggcattcc
     1261 acgccaggtc aacacccaga tgatggccac cagcagcgcc tggaaccaca catcggacat
     1321 gatgttgtgc tcgatctgca gctggtaggc atcgagcagc accggcgcgg tcgccagcgc
     1381 agccagccac cggcgggcac cgtggcgcag cagcaacaga tagatcgcca ccgcaatgcc
     1441 caagccgagc aggtgttgca cgattaccac cgcgcgcagc ccgcccaacg gcagcacgat
     1501 ggtgaggatc aggtggtagc cgatcgggcg caaaccgttc ggacgcagat cgtcgatatt
     1561 gttcaggtag ctgaaggagt cgatgtagag gatcgcgggc tggtaggcca gcacggtgat
     1621 cacccgcaac gcggcacccg ccgcgagcag caccagcagc gtccaatgcc cccgcagcca
     1681 ccgccgcgtg cggaaagcca gtgctgcgcc gggcttcggg gcgccaacgt gggcgggcgc
     1741 cagcaggtcg gtcaccggtc cggagtgcat gcgtcggtgg tgactgtgct gattaccgtg
     1801 tcggcgaccg tgaagtcctt ccataccctc gccagcccgt cggctagcga caccgacggc
     1861 caatagccca gctgctcgcg ggcgtgacag atgtcgacga tcaccgcggg catctcaccg
     1921 ggcttggcct cgacgtgttc ggcgggcagc gccgcgccgg tgaccatgcg cgccgcctcc
     1981 aagagctcca gcacggtcac cgactggcct gagccgatga tcgcagtacc ggtgaagcgc
     2041 cggtcccagg ccgaaaccac ggcctggcac acgtcatcaa cgtggaccag gtcgcgccgc
     2101 tgcagcccgt ccccgtagat ctggacaccc cggccggcca atgcagcgcg catcatccgc
     2161 ggcacgaagc tgtctttgtg ctccatgccg gggccgtaga tgttggtgaa ccgcaacgcg
     2221 ctggcggcca tgccgtaggc acccgcatag ccgtgcaaca gcatctcgca ggccgccttg
     2281 gtggcgccgt agggagtcaa tgggcgcagc ggtgctgtct cgtccatcgt ggcgtagccg
     2341 acgtcgccga ccactgcgtt ggtggaggcc atgacgaagc gccgcacacc ggcctgccgg
     2401 cagtattcga gcagatcctg ggtcactgcc acgttcgcct ggtacgtggc ggccggatgc
     2461 tgcaccgagc gcaacactga cgtgagtgcg gccaggtgca ccacaccggc cacatccccg
     2521 ccgtccaacg cctcagcgat cagccctgga tcggtgagat caccgacgac gctgtgcacc
     2581 gccgggtcgg gatgagcccg gcagtccacc acggtgaccg ggttgccccg gccgatgaac
     2641 gcggcgactg cggcacgacc gacgaaacct g
//
LOCUS       DATADN010000915         2118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_715247, whole genome shotgun sequence.
ACCESSION   DATADN010000915 DATADN010000000
VERSION     DATADN010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715247"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..430)
                     /locus_tag="VHH53_11890"
     CDS             complement(2..430)
                     /locus_tag="VHH53_11890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX2290866.1"
                     /translation="MPTLFSRIILGEIPGRFIWTEQRCVAFLTIAPLQPGHALVVPRD
                     EIDEWTDADDDVVARLMVVAKQIGAAQKTAFSAPRAGLIIAGFEVPHLHVHVFPSWGI
                     DDFDFDKADRDPDPAELDAAAEALRAALRTAGHGDRIPSN"
     gene            complement(470..901)
                     /locus_tag="VHH53_11895"
     CDS             complement(470..901)
                     /locus_tag="VHH53_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290867.1"
                     /translation="MITPVQVLVDADNLDVPRLRLLVAALEAVLEASLSTDVVVAGAP
                     AALDALDWPPAAQVLPASGWQSADLLLARAYRTDEHPLLLATGDGDFVQLARRHPGPV
                     LLVSGTSSRSRAFIGPRISTTDPASDGGAQLRSWLGQSTVL"
     gene            959..>2118
                     /locus_tag="VHH53_11900"
     CDS             959..>2118
                     /locus_tag="VHH53_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX2290868.1"
                     /translation="MAAGRLSGLDTALLCLDRDITPMNLGGLAIFRSARPPDPRRIAC
                     LLAGRAQRQPLLRQRVQPALSALAAATWAEDPGFRAADHIHLHHVDGPGSADKAAGKV
                     AALAAELMVQPLPRTRPLWEFHVISGMGGNRFAILVTMHHAFGDGLAALDISLRMLDE
                     FRPRHGGALTPPSSAPPSLTQPSMQSRLWRVPGDLRGAVEGVVEQMAEATGIVASVLR
                     RVRLPPPGSPLVISSSGQRALATARLDLRQIRRIRGHSGGTVNDVLLSVVAGALREWL
                     IARGDLAEGSVLRAFVPVSRRARSSDRMGRNQLSGYLCELPVGEPDPGKRLLRIRSAM
                     ERNKAAGTDRGVGAIPLLADRLPPAVHRVAAPVAGEGAVLLFDLIVTSIPLPA"
BASE COUNT          335 a          697 c          726 g          360 t
ORIGIN      
        1 tttagttgct gggtatccgg tcaccgtgcc ctgccgtccg tagcgccgca cgcagcgcct
       61 ccgcggcagc gtcgagttcg gccgggtcag gatcccggtc ggccttgtcg aagtcgaagt
      121 cgtcgatgcc ccaggacggg aacacatgca cgtgcaggtg tggcacttcg aacccggcga
      181 ttatgaggcc cgcgcgtggc gcgctgaagg cggtcttctg cgccgcgcca atctgcttgg
      241 ccaccaccat cagccgagcg accacatcgt cgtcggcgtc ggtccactca tcgatctcat
      301 cgcgggggac gaccagggca tggccaggtt gcagcggagc gatggtgaga aaggcaacgc
      361 agcgttgctc agtccatatg aagcgacccg ggatctcacc gaggatgatg cggctgaaca
      421 gggtaggcac gattccctcc ggtgatctcg tgtcccgatg atctcctcat cacaggacag
      481 tggattgtcc caaccaggac cgcagctgcg caccgccgtc ggacgccgga tcggtagtac
      541 tgattctggg gccgatgaac gcccgtgagc ggcttgacgt gccgctgacc agcaacaccg
      601 ggccggggtg gcgtcgcgcc agttgcacga agtcgccgtc cccggtggcc agcagcagcg
      661 ggtgttcatc ggtccggtaa gcgcgggcca gcagcaggtc tgcactctgc caaccgctcg
      721 ccggcaacac ctgagcagcc ggcggccagt cgagtgcatc caatgctgcc ggcgcgccgg
      781 ccaccacgac gtcggtggac agcgacgcct ccaggacagc ctccagcgct gcgaccagca
      841 accgcagccg gggcacgtcg aggttgtccg cgtcgaccag aacttgcacg ggtgtgatca
      901 tgcacctgtg caaggctccg atgagacgcg gggaatgcgt gatcgcagga ggcttgccat
      961 ggcagcgggg cggctcagcg ggcttgacac cgccttgctc tgcctggatc gcgacatcac
     1021 cccgatgaac ctcggtggct tggcgatctt ccggtcggcc cggccaccgg atccccggcg
     1081 gatcgcctgc ttactggccg gccgggccca gcgtcaacca ctgctgcgac agcgggtgca
     1141 accggccctg tcggcgctgg ccgcagcgac gtgggccgaa gatcctggat tccgcgccgc
     1201 ggatcacatt cacctgcatc atgttgacgg tcctggcagt gctgacaagg cagccggcaa
     1261 ggtagcggca ttagccgccg agctgatggt ccaaccgctg ccgcgaaccc gtccgctgtg
     1321 ggagttccac gtcatatcag gtatgggcgg gaaccgtttc gcgatactgg tcacaatgca
     1381 tcatgcgttc ggcgacgggt tggccgcatt ggatatcagc ctgcggatgc tggatgaatt
     1441 cagaccacga cacggtggcg ccttgacgcc gccatcgtcg gccccgccat cgttgacaca
     1501 gccgagcatg cagtcccggt tgtggcgcgt gcccggtgac ctacgcggtg ctgtcgaggg
     1561 cgtggtagag cagatggcag aggccaccgg catcgtcgct tcggtgcttc gtcgggtgcg
     1621 cttgccccca ccgggctcac cgctggtgat ctcgtcttcg ggtcagcgtg cgttagcgac
     1681 ggctcggctc gatctccggc agatacgccg gatccgggga cattccggcg gcacggtgaa
     1741 cgacgtcctg ctgtcagtgg tggccggcgc attgcgggag tggctcatcg ctcgcggcga
     1801 cctggccgaa gggtcggtgc tccgggcgtt cgtcccggtc agccggcggg ccaggtcgag
     1861 cgaccgaatg gggcgaaatc agctgtccgg ctacctctgc gagctgccgg tcggcgagcc
     1921 cgatccgggc aagcggctgc tcaggatccg ctcggcgatg gagcgcaaca aggcagccgg
     1981 caccgaccgc ggcgttggtg cgataccact gctcgctgac cggttgccgc ctgcggtgca
     2041 ccgcgtggcc gcgcccgtgg ccggtgaggg cgctgtactg ctgttcgacc tcatagtgac
     2101 cagcatcccg ctgccggc
//
LOCUS       DATADN010000916         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_855230, whole genome shotgun sequence.
ACCESSION   DATADN010000916 DATADN010000000
VERSION     DATADN010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855230"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VHH53_11905"
     CDS             <1..418
                     /locus_tag="VHH53_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX2290869.1"
                     /translation="TSLIAVLPVLGLLVIGVGLLGVGTLKDLALVQMVGILAGAMSSI
                     FLATPLMVDLTMRRPAYRQQAERVLQRREKLASKANPEHEDEVSSTSPRARTAEGMAA
                     GVPARVGKTAPAGRRRGHPAGKSSRPSGKAGRSSGS"
     gene            667..>1585
                     /locus_tag="VHH53_11910"
     CDS             667..>1585
                     /locus_tag="VHH53_11910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2290870.1"
                     /translation="MAGWSVPGVVHLREVREDPAGRRVLARHRVTRRSLAVTYLSEEL
                     RTDAEFRARFAREFGRLAQVRDARVSRVHRYVEYHHGAAVIGDHVSGTSLRALLLAQG
                     ATGIEAALVVLKDTLRGLAAYHAAGLAHGDIKPECVGLTRAGRVRLVDVGLSTAHGRQ
                     LLARSTPFYLAPEQWNDRSASPTGDVYAATVTFFECLIGAPPFYAGSSTELAVKHHQS
                     SPPLDVLPAPLRELVVRGLAKDPHVRPAARNLIAHVDDVATRAVGTGWERRGRRELAG
                     LLSSRSALPDRPKPARRTGGMGREPRQPVR"
BASE COUNT          233 a          520 c          553 g          279 t
ORIGIN      
        1 cacctcgctg atcgcggtac ttcccgtgct gggtctgctg gtgatcggtg tcggcctact
       61 gggcgtggga acgctcaagg accttgcatt ggtgcagatg gtcggcatcc tggccggggc
      121 gatgtcctcg atctttttgg cgacgccgtt gatggtcgac ctcaccatgc gccggccggc
      181 ttaccgccag caggctgagc gggtgctcca gcgccgggag aaactggcca gcaaagccaa
      241 tcccgaacac gaggacgagg tctcgtcgac gagcccacgg gccaggacag cggaaggtat
      301 ggccgccggt gtgcctgccc gggtcggcaa gaccgcgccg gccggacggc gacgcgggca
      361 ccctgcgggc aagagcagca gaccctctgg caaggccggc aggtcgtcag gttcttgacc
      421 gtgcaccgct cacctgatcc tgatcgtccc gtcactggca gtcttggtca ttgcgcagtg
      481 tagcgctttg gtgacgctgc gctacgtgct tgcttctttt caccgtctat ttggtgagtt
      541 gtcgctctga gtacttgcct ctggcgctca ttaggggaac gctcagtagt caagcgccgg
      601 atgtagctcc ggtgagtgag gggcacggga gggggcaccg tggggggcga ccaggaaaga
      661 tcggggatgg cggggtggtc tgttccgggt gtggtgcatc tccgcgaggt gcgcgaagac
      721 ccggccggtc ggcgggtatt ggcccgtcac cgcgtcaccc gcaggtcgct ggcggtgact
      781 tacctgtcgg aagaactgcg caccgacgcc gagttccgcg ctcgcttcgc ccgggagttc
      841 ggacggctgg cccaggtccg cgatgccaga gtgtcacggg tccatcgata cgttgagtat
      901 caccatggcg ctgccgtcat cggcgaccat gtcagcggaa cttcgctgcg tgctctgctg
      961 ctggcacagg gagccaccgg tatcgaggcg gcactggtcg tactcaagga cacgctgcgc
     1021 ggcctggccg cctaccacgc cgcagggctg gcgcacggtg acatcaagcc ggagtgcgtg
     1081 ggcctcaccc gtgctggccg cgtccggctc gtcgacgtcg gactgtccac cgcccacggc
     1141 aggcagctgc tcgctcggtc gacaccgttc tacctggctc ctgaacagtg gaacgatcgt
     1201 tcggctagcc ccaccgggga cgtgtacgcc gccacggtca cgttcttcga gtgcttgatc
     1261 ggcgcgccgc cgttctacgc cggcagctcg accgaactcg cggtcaagca ccaccagagc
     1321 agtccccccc tcgatgtgct tcccgcaccg ctgcgcgagc tggtggtgcg cggccttgcc
     1381 aaggacccgc acgtccggcc cgcggcgcga aacctcatcg ctcatgttga cgacgtggcg
     1441 acccgagctg tgggcactgg ctgggaacgg cgcgggcgac gcgagctggc cggtttgctg
     1501 agcagccgtt cggccctgcc ggaccgaccc aagccggctc ggcgcacagg cggcatgggg
     1561 cgtgagcccc ggcagccggt ccggc
//
LOCUS       DATADN010000917         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1306852, whole genome shotgun sequence.
ACCESSION   DATADN010000917 DATADN010000000
VERSION     DATADN010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306852"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VHH53_11915"
     CDS             <1..206
                     /locus_tag="VHH53_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290871.1"
                     /translation="RMSEAGRAGQLEARQQLYDYVDGLWADIKRAGESPAVGDKYQAI
                     AAMRELTGMLRTIAFEAVYDAPQ"
     gene            297..539
                     /locus_tag="VHH53_11920"
     CDS             297..539
                     /locus_tag="VHH53_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290872.1"
                     /translation="MTDHATPHGHDGGLFRTPGQTDGQQVASGVESVKPVERPSRDLF
                     HQAGGHDTAEHRHPAAQRDAAVMRSLQFPFDDGDCG"
     gene            585..>1716
                     /locus_tag="VHH53_11925"
     CDS             585..>1716
                     /locus_tag="VHH53_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2290873.1"
                     /translation="MVSVAAHPVGRRGTKAPEKTQEAPVKEVDGAGEPSSTSIDPSDD
                     SLPLALEDAEDPQEPVDNVEPADLVWDEEESEALRQARKDAELTASADSVREYLKQIG
                     KIALLNAEEEVELATRIEAGLYAAERVRRSDDVTEKLSPQLRRDLRWIVRDGERAKNH
                     LLEANLRLVVSLAKRYTGRGMPFLDVIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIR
                     QAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDISAEKV
                     LEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAIIAVDAVSLTLLQDHLQSVLATLS
                     EREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIESKTM"
BASE COUNT          342 a          547 c          578 g          249 t
ORIGIN      
        1 tccgaatgag cgaggcgggt cgggctgggc agctagaagc ccgccagcag ctctatgact
       61 acgtggacgg gttgtgggca gacatcaagc gggccgggga gagccccgcc gtaggagata
      121 agtaccaggc gatcgccgcg atgcgggaac tgaccgggat gctacggacg atcgctttcg
      181 aggcggtgta cgacgcaccg cagtgagtcc gacgacgtgg cacgacacca caccgcgacg
      241 tcgcgcggcg gatactcgcg acccacacag cactcacgac cgacgaaggg cgcaacttga
      301 ctgatcacgc aacaccgcac ggccatgacg gcgggttgtt ccgcacaccc gggcagacag
      361 acgggcagca ggtggcgtcc ggcgtcgaga gcgtcaagcc ggttgagcga ccatcccggg
      421 atctgtttca ccaggctggt ggccacgaca ctgcggagca tcgacacccg gctgctcagc
      481 gcgatgctgc ggtgatgcgg tcgctacagt tccccttcga cgacggtgac tgtggctaga
      541 tcggagatca tcggtgtcgc cggaattcgg gttaggaggt caggatggtg agcgtcgccg
      601 cgcatcccgt aggccgccga ggaactaagg caccagagaa gacgcaggag gcgccggtca
      661 aggaggtgga cggggccggt gagcccagtt cgacgtcgat cgacccgtcc gatgacagcc
      721 tcccgctagc tttggaggac gccgaggatc cccaggagcc tgtggacaac gtcgagccgg
      781 cggatctcgt ctgggacgag gaggagtccg aggcgctgcg gcaggcccgc aaagacgccg
      841 aactcaccgc gtcggccgac tcggtacggg agtatctcaa gcagatcggc aaaatcgcgc
      901 tgctcaatgc cgaggaggag gtcgaactcg ccacccgcat cgaggccggt ctctacgccg
      961 ccgagcgggt acgcaggtcc gacgacgtca ccgagaagct gtccccgcag ctgcgccggg
     1021 acctgcgctg gatcgtccgc gacggcgagc gcgccaaaaa tcacctgctg gaagccaacc
     1081 ttcgcctggt cgtgtcgctg gccaagcgct acaccggccg cggcatgccc ttcctcgacg
     1141 tgatccagga gggcaacctg ggcctgatcc gtgcggtgga gaagttcgac tacaccaagg
     1201 gatacaagtt ctccacctac gccacctggt ggatccgcca agccatcacc cgcgccatgg
     1261 ccgaccaggc ccgcaccatc cggatcccgg tgcacatggt cgaggtgatc aacaagctcg
     1321 gtcgcatcca acgcgagttg ctccaggacc tgggccgcga gcccaccccc gaagagctgg
     1381 ccaaggagat ggacatctcc gcggagaagg tgctggaaat tcagcagtac gcccgcgaac
     1441 ccatctcgct ggatcagacc atcggcgacg agggcgactc ccagctgggc gacttcatcg
     1501 aagactccga ggccatcatc gcggtcgacg cggtctcatt gacgttgcta caggatcacc
     1561 tgcagtctgt gctggccacg ctgtccgagc gtgaggccgg tgtggtgcgg ctgcgattcg
     1621 ggctcaccga cggccaaccc cgcacgctgg atgagatcgg gcaggtctac ggggtgaccc
     1681 gggaacggat ccggcagatc gagtccaaga cgatgg
//
LOCUS       DATADN010000918         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_422667, whole genome shotgun sequence.
ACCESSION   DATADN010000918 DATADN010000000
VERSION     DATADN010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422667"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..>1626)
                     /gene="ilvD"
                     /locus_tag="VHH53_11930"
     CDS             complement(109..>1626)
                     /gene="ilvD"
                     /locus_tag="VHH53_11930"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX2290874.1"
                     /translation="EITPCNLSLDRLAKASKEGVHAAGGFPMEFGTISVSDGISMGHV
                     GMHYSLVSRELIADSVETVVEAERLDGTVLLAGCDKSLPGMLMAAARLDLAAVFLYGG
                     TILPGRVDDREVTIIDAFEAVGACARGLISRAEVDAIERAICPGEGACAGMYTANTMA
                     CAAEALGMSLPGSASPPAVDRRRDGFARASGSAVVDLIARGITTRQILTRPAFENAIA
                     VVMAFGGSTNAVLHLMAIANEAGVPLELDDFNRVGDRVPHLADVKPFGKHVMATVDQV
                     GGVPVVMKALLDAGLLHGDCLTVTGRTVAENLTELAPPDLDGKILHRLTDPIHPTGGI
                     TVLRGSLAPEGAVVKSAGFDSARFAGRARVFDGEQAAMDAIPHLQPGDAVVIRYEGPQ
                     GGPGMREMLAVTGAIKGAGLGKDVLLLTDGRFSGGTTGLCVGHVAPEATHGGPIALVR
                     DGDSIVLDMDRRELNLQVDAGELARRRQEWKAPQRPLRGVLAKYARLVVPTRHRS"
BASE COUNT          254 a          591 c          536 g          245 t
ORIGIN      
        1 ctgcgcggcg tgctggccaa gtacgcccgg ctggtcggct ccgccgcgga aggcgccgtc
       61 tgtggataac tcgtgagtgg gtaacaatgt catgacgctg ttacccactc acgagcggtg
      121 ccgggtgggc acgaccagcc gggcgtactt ggccagcacg ccccgcagcg ggcgctgcgg
      181 ggccttccac tcctgccgcc gccgcgccag ttccccggcg tcaacttgca ggttcagctc
      241 gcgacggtcc atgtccagca cgatcgagtc gccgtcgcgg accagcgcga tcggcccgcc
      301 gtgggtggcc tcgggcgcca cgtgcccgac gcacaatccg gtggtgcccc cggagaaacg
      361 cccgtcggtg agcagcagaa cgtccttgcc caatccagcg cccttgatcg ctccggtgac
      421 cgcgagcatt tcgcgcatcc cagggccgcc ctgcggaccc tcataccgga tcaccacggc
      481 gtcgccgggc tgcaggtgcg ggatggcgtc catcgcggcc tgctccccat caaacacccg
      541 agcacgcccg gcgaatcggg ctgagtcgaa gccggcactt ttcaccaccg cgccctcggg
      601 agccaacgag ccgcgcagga cggtgatccc tccagtggga tggatcgggt cggtgaggcg
      661 gtgcaagatc ttcccgtcca ggtccggcgg ggccagctcg gtgaggttct cggcgacggt
      721 gcgcccggtg acggtgagac agtcgccgtg cagcagtcct gcgtccagca gcgccttcat
      781 gaccaccggc acgccgccga cctgatcgac agtggccatc acgtgcttgc cgaagggttt
      841 gacgtcggcc agatgcggca ccctgtctcc cacccggttg aagtcgtcca gttccagcgg
      901 caccccggcc tcgttcgcga tagccatcag gtgcagcacc gcgttggtcg agccaccgaa
      961 ggccatcacc accgcgatcg cgttctcgaa tgccggacga gtgaggatct gccgggtggt
     1021 gatcccgcgg gcaatgagat ccaccacagc ggacccgctg gcccgtgcga acccgtcgcg
     1081 gcgccggtcc accgcgggag ggctggccga gccgggcagc gacatgccca gcgcctcggc
     1141 cgcgcaggcc atcgtgttgg cggtgtacat gccggcacag gcgccctcac cgggacagat
     1201 cgcccgctcg atggcgtcca cttcggcccg gctgatcaaa ccgcgggcgc aggcccccac
     1261 cgcctcgaag gcgtcaatga tggtgacctc acggtcgtcc acccggccgg gcaggatggt
     1321 gcctccgtag aggaataccg ccgccaggtc gagtcgggcc gcggccatca gcattccggg
     1381 caggctcttg tcgcagccgg ccagcagcac cgtcccgtcc agccgctcgg cttccaccac
     1441 ggtctccacc gagtcggcga tcagctcacg ggacaccagc gagtaatgca tccccacgtg
     1501 gcccatggag atcccgtcgg atacggagat cgtgccgaac tccatcggga accccccggc
     1561 ggcgtgcacg ccttccttgc tggccttggc aagccggtcc agcgacaggt tgcagggagt
     1621 gatctc
//
LOCUS       DATADN010000919         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1306908, whole genome shotgun sequence.
ACCESSION   DATADN010000919 DATADN010000000
VERSION     DATADN010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306908"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VHH53_11935"
     CDS             complement(<1..266)
                     /locus_tag="VHH53_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290875.1"
                     /translation="MPPSSGRSSRNKAARDGSGQGRCGRRRAGHGSEIKSGDINSGDG
                     KGDAKAGVKAEGTSSGQSRRRTGRMRTVDETSAGGLVVDTETGHA"
     gene            500..>1590
                     /locus_tag="VHH53_11940"
     CDS             500..>1590
                     /locus_tag="VHH53_11940"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX2290876.1"
                     /translation="MADELAARFIAAGHSLYLVGGSVRDALLGRLGSDLDFTTDARPD
                     ATRRILDGWADAVWDTGIAFGTIGATKAGTHCEITTYRADRYDRVSRNPDVVFGDTVE
                     DDLARRDFTVNAMAVQLPLHDGGGRFIDPHGGVAALARRELNTPATPQESFADDPLRM
                     LRAARFVSQLGFTLAPRVQAALVEMAPEIRRITVERVAAELSKLLLGADPRAGVELVV
                     DTGLADHVLPEVPAMRLEIDEHHQHKDVYVHSLTVLDQAIALEDGEPDLVLRLAALLH
                     DIGKPATRRHESGGGVSFHHHEVVGAKMVRKRLRALRYPTHVVEDVAKLVFLHLRFHG
                     YGSGEWTDSAVRRYVTDAGDLLPRLHKLVR"
BASE COUNT          226 a          556 c          535 g          273 t
ORIGIN      
        1 gcgtgcccgg tctcggtgtc gaccaccagc ccgccggccg aggtctcatc cacagtgcgc
       61 atccggccgg ttcggcgcct gctctgcccg gagctggtgc cctctgcctt aacccccgcc
      121 ttcgcgtccc ctttaccgtc accgctgttg atgtcaccgc tcttgatctc actgccgtgg
      181 ccggccctgc ggcgaccgca gcggccctgg ccgcttccgt cccgggcagc cttgttacgg
      241 ctggacctac cggaggacgg gggcatggcg ctgatggtat aggcgaagtc cgtcctggct
      301 gctgcggtgc ggtcctgtac gcagcagcga ccggtcgagc acctccgcga tcaccgccgg
      361 cgcggtcgca ggcgtcgggc cgttacgctg actcgccgtg tcgccttcgc cttctgtgac
      421 ctctgaacga ggaaaccgca ccccgttgct gagcacggca cagcagaatg cggttgtgga
      481 gctgctccgg atcgcgccga tggccgatga gctagccgcc cggttcatcg cggccggtca
      541 ttcgctgtac ctggtgggcg gcagtgtgcg agacgccctg ctcggcaggc tgggcagcga
      601 cctggatttc accaccgacg cccggcctga cgccacccgg cgaattctgg acggctgggc
      661 cgacgcggtg tgggacaccg ggatcgcctt cggcaccatc ggtgcgacca aggccggcac
      721 ccactgcgag atcacgacct accgggctga ccgctacgac cgggtctccc gcaatccgga
      781 cgtcgtcttc ggtgacaccg tcgaggacga tctcgcccgg cgcgacttca ctgtgaacgc
      841 gatggctgtc cagctgccgt tgcacgatgg cggcgggcgg ttcatcgacc cgcacggcgg
      901 ggtagccgcg ttggcccgcc gtgagctaaa cactcccgcg acaccgcagg aatccttcgc
      961 cgacgatccg ctgcggatgc tgcgtgcggc ccggttcgtc tcccaactcg gtttcaccct
     1021 cgccccgcgg gtgcaggccg cgctggttga gatggcaccc gagatccgtc gcatcaccgt
     1081 cgaacgggtg gccgcggagc tgtccaagct gctgctcggt gcagacccgc gggccggtgt
     1141 tgagctggtg gtggacaccg ggctggccga ccacgtgctg cccgaggtac ccgcgatgcg
     1201 cctggagatc gatgagcatc atcagcacaa ggacgtctac gtacactccc tgacggtgct
     1261 cgaccaggcg atcgcactgg aggacggcga gcccgacctg gtgctgcggc tggcggcgct
     1321 gctgcatgac atcggcaagc cggctacccg caggcacgag tccggcggcg gagtgagctt
     1381 ccaccatcac gaggtggtcg gggccaagat ggtccgcaag cggttgcgcg cgctgcgtta
     1441 tccgacgcac gtcgtcgagg acgtcgccaa actggtattc ctgcacctgc ggtttcatgg
     1501 ttacggcagc ggtgaatgga ctgactcggc ggtgcgccgc tacgtcaccg acgccgggga
     1561 cctgctgccg cggctgcaca agctcgtccg
//
LOCUS       DATADN010000920         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1475531, whole genome shotgun sequence.
ACCESSION   DATADN010000920 DATADN010000000
VERSION     DATADN010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475531"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..545
                     /locus_tag="VHH53_11945"
     CDS             285..545
                     /locus_tag="VHH53_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290877.1"
                     /translation="MTVFDTLRAAAHALSDRTDRAHAPALAGLLQNRADDMEGNIAIW
                     QRTGQDIPALVEKHYGVYLTVARAVLFDPRYLNSAAPSPQAN"
     gene            complement(1080..1237)
                     /locus_tag="VHH53_11950"
                     /pseudo
     CDS             complement(1080..1237)
                     /locus_tag="VHH53_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007053875.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1438..2640
                     /locus_tag="VHH53_11955"
     CDS             1438..2640
                     /locus_tag="VHH53_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2290878.1"
                     /translation="MAEALVEAPFSITDGAQRHIRYAQLTAAGPVHHIVLPTGHPAWL
                     ITGYHEVRQALHAGQLVRSDAAEAVLRRDWLSPEVCAAMGNHMASRNPPGHTRLRRLV
                     TAAFTRRRIEQLTPRIQEITDELIDAMANEAQVDLITAFAYPLPITVICELLGVPADH
                     RAEFHHWSTTLVAGALADPDTYRSAATALVRYLQELLTIKRDTPTDDPLSALLMVRDG
                     QDRLCEDELTSMVALLLIAGHETTVNVIGNGMHALLSHPDQLALLRAHPQRLGTAVEE
                     LLRFDGPFQVAPFRVTTESIGIGGITIPAGDIVVPGLLAANRDPAGTANPDTLDITRT
                     SNPHLAFAHGIHHCLGAPLARLEARIALGTLLDRFPRSRLAVPAEALIWRPSVLMNGL
                     DTLPVVLR"
BASE COUNT          513 a          877 c          832 g          514 t
ORIGIN      
        1 cgccgaccga ggcagacgtg ctgcaggtcg acgacgcatc aacacagcgg gccggagctt
       61 gtgagcggtc aacgcaacag tgcccaacgg gctgctgaat agatccggcg cctagcggtc
      121 acaaggaggg ttgacttccg atgaccagaa ttgagactgc cgtcatggcc gtcaggttct
      181 actgtgaaac ggcttcaagg tgagcattga tagtcaagga cctacacacc agcgtgtcgc
      241 caccgagcga acagtcgccg cgaagccgag acagaggtgg tggcatgacc gtttttgaca
      301 cgctgagagc agcagcccac gcgcttagcg accgtaccga ccgtgcacac gcacctgccc
      361 tggctggctt gctgcaaaac cgtgccgatg atatggaagg caatatcgcc atctggcagc
      421 gcaccgggca agacatccct gctctggtag aaaagcacta cggtgtgtac ctcaccgttg
      481 ccagagctgt tcttttcgat ccacgttacc tgaacagcgc cgcaccatct ccacaagcaa
      541 actgacacaa ccccagcacc gcagcgtcgc aaatagacga gcagacgcca cgcactcatg
      601 agggtgcctc gaaggtcggg gcatctctcc atcctccgct taggacagag gctcatcaga
      661 cccggggtgg tccacggtgg gtggatgccg ttgtgagaga acagtatctg acgccagttg
      721 tcgctcgtct tgtcctcact tgccttgact agaaatgctt tggtgccttc cagtaggtgc
      781 cgcgtgcctg ttcggccttg acttgcacgg tttgttggcc tgtgggctca tgcttccacg
      841 gtgtgaccga gctgctcgcg ggttcgttcg atgttctggc gtagctgatc ctcgcgtgac
      901 gaggaagtca ttggtgcatc gcttccatga tggtcttgat gtcgattttg acggctgccg
      961 cgtgcgcaca ccgccgtcct gaccgtcgag gacggctcag cactacacgc gcgcttggtc
     1021 acgcccgatc gcaatgtaga tgggctggtt gcccagtgac cggttttgca ttcgatcatc
     1081 tctttgacga ccttgtcggg gatccgggag atggtgtcgc ggctgacctt cgtgccgcag
     1141 atctcataaa gtggcgccgc gatctccccg tcatcaaccc gctggcggtc agcgacagca
     1201 cgatcgcatc gatgccatca aggcgccgtt gccgcttgcc tcacaaggcg gcgaacacaa
     1261 gatcacgtgt ggctgtgcct tagcagcacc ggatcgccac acgaaggaca gacagctacc
     1321 tcattccgag atccacggcg gtcagaaagg aacccaaagc ggtatcacga gccggggaag
     1381 gcggctcttc ttcgttacgt atgccgccga gatgagggtg cccaggaggc gcaggccatg
     1441 gctgaggcac tggtggaggc gccattttcg atcaccgacg gggcgcagcg ccatattcgg
     1501 tatgcgcagc tgacggccgc tggcccggtc caccacatcg tgttgcctac gggacatccg
     1561 gcttggttga tcaccggcta ccacgaagtg cggcaggccc tgcatgctgg gcagctggtc
     1621 aggagtgatg cggcagaggc ggtgctgcgc cgcgactggc tgtcgcctga ggtgtgcgcg
     1681 gcgatgggca atcacatggc tagtcgcaac ccgcccggtc acacccggct gcgccggctg
     1741 gtcaccgcgg cattcacccg ccgtcgcatc gagcagctca caccgcgtat ccaggagatt
     1801 actgatgagc tgatagacgc aatggccaac gaggcacagg tcgacctgat caccgcgttc
     1861 gcctacccac tgccgatcac cgtgatctgc gagctgctgg gcgtgcccgc ggatcaccga
     1921 gcggagtttc accactggtc aacgacgttg gtggccggcg cactggccga cccggacacc
     1981 taccgctcgg cggccaccgc gctggtgcgc tacctccaag agctgctcac catcaagcgc
     2041 gataccccca ccgatgaccc gctctcggcc ctattaatgg tccgcgacgg ccaagaccgt
     2101 ctctgcgagg acgagctcac ctcaatggtg gccctgctgc tcatcgccgg ccatgagacc
     2161 acggtcaacg tgatcggcaa cgggatgcat gcactgctta gtcaccccga ccagctggcg
     2221 ctgttgcgcg cccacccaca gcggctgggc accgcagttg aagagcttct gcgcttcgat
     2281 ggcccatttc aggtcgcacc cttccgagtc accaccgagt caatcgggat tggtgggatc
     2341 accatcccgg ccggtgacat tgtggttccc ggcttgcttg ccgccaaccg cgatccagcg
     2401 ggcaccgcca accccgatac gctggacatc acccgcacat ccaatccgca cctggccttc
     2461 gctcacggta tccaccactg cctaggcgcc cccctggcgc ggctggaagc ccggatcgcc
     2521 ctaggcacgc tgcttgaccg tttcccacgg tcgcgcctgg cggtgcctgc cgaggcgctg
     2581 atttggcgac ccagcgtgct gatgaacggc ctggacacgt tgcccgtggt cctgcgctga
     2641 aacaggcacg gctatcaaga cagcccaagg ttggtgatca atgtcggcgt ggggcgctgt
     2701 gggtggtttg gagacctgtg aggaggacgt cgagac
//
LOCUS       DATADN010000921         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_715812, whole genome shotgun sequence.
ACCESSION   DATADN010000921 DATADN010000000
VERSION     DATADN010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715812"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VHH53_11960"
     CDS             complement(<1..297)
                     /locus_tag="VHH53_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX2290879.1"
                     /translation="MASRAASGGEGFAAIRTEFGLPVRFPDPVLAEAEQAVAAWGRGG
                     AGSGAREDATDLPLVTIDPPGSKDLDQAVLVQARPGGGFRVHYAIADLGSFVSPD"
     gene            complement(363..848)
                     /locus_tag="VHH53_11965"
     CDS             complement(363..848)
                     /locus_tag="VHH53_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290880.1"
                     /translation="MYTSDPIPRPAAPPATGTPLRDYLVNHGGPGVQENYLVMPRSLI
                     EAMPLPWQNAMVQICAEFQQAFGHLSWPQYRVVPSRKERLVNLDEDQLAEVGYLVEID
                     SDGEVVYRARGGEPIPNPEETEVLVSFQDPIPIEQNNANQETPPRGFPAPPIRQQTNW
                     T"
     gene            complement(885..>1716)
                     /locus_tag="VHH53_11970"
     CDS             complement(885..>1716)
                     /locus_tag="VHH53_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX2290881.1"
                     /translation="AADAARLKEDQRQNVALIIQIGKDRQLPPLAWQIAIQAGMTESG
                     LRSLNYGDRDSLGMFQMRPSMGWGTPAQVTNPVYAINKFYDVLNGVPNWQEQRPGDSA
                     QDVERSAFPDRYHRWEAMAAHLIGNIGAVEDPTGCGEGTGNLLPAPTQAAGSAIQFAL
                     AQQGKPYQWGAATTHQNSFDCSSLMLQSYRSAGVLLPRTSREQWRAGAYLPVQQAQPG
                     DLLFWAYDTSNPATIHHVAMYLGDNKIVEAQQTGVPVHIRPVRWTEGGLMPQAVRPGV
                     "
BASE COUNT          251 a          608 c          591 g          266 t
ORIGIN      
        1 gtccgggctg acgaacgaac cgaggtcggc aatcgcgtag tgcacccgga aaccgccgcc
       61 gggccgagcc tgcaccagga ccgcctggtc gaggtccttc gagccgggcg ggtcgatggt
      121 caccaggggc aggtcggtgg cgtcctcacg ggcgccggaa ccggcaccgc cgcgccccca
      181 cgcggccacg gcctgctcgg cctcggccag gacggggtcg gggaaccgca cgggaaggcc
      241 gaactcggtc cggatcgcgg cgaaaccctc gccgcccgac gccgccctcg aagccacggg
      301 ggcaccctat ccccggtgcc cgccacgcca cgggaccggt gaggcaaagt cgcgggagac
      361 gatcaggtcc agttggtctg ctggcggatc ggcggggccg ggaagccgcg cggcggggtc
      421 tcctggttgg cgttgttctg ctcgatcgga atggggtcct ggaaggacac caggacctcg
      481 gtctcctccg ggttgggaat cggttcaccg ccccgggccc ggtagaccac ctctccgtcg
      541 gagtcgatct ccaccaggta gccgacctcg gccagctgat cctcgtcgag gttcaccaga
      601 cgctccttgc gcgacggcac cacgcggtac tgcggccagc tcaggtgccc gaacgcctgc
      661 tggaactccg cgcagatctg caccatggcg ttctgccacg gcaacggcat cgcctcgatc
      721 aacgacctcg gcatgaccag atagttctcc tggacgcctg gtccaccatg gttgaccagg
      781 tagtcgcgca gtggcgtgcc ggtcgccggc ggcgctgcag gacgcgggat cgggtcggac
      841 gtgtacatgg tcacccctgc gcactcccct actacggaac cagttcaaac ccccggccgg
      901 acggcctggg gcatcagccc accttcggtc cagcgcaccg gccgaatgtg caccggcaca
      961 ccggtctgct gggcctcgac gatcttgttg tcaccgagat acatcgccac gtggtggatc
     1021 gtcgccgggt tgctcgtgtc gtacgcccag aacagcaggt cacccggctg cgcctgctgg
     1081 accggaaggt aggcgccggc ccgccactgc tcccgcgagg tccgcggcaa cagcaccccg
     1141 gccgaccggt aggactgcag catcaggctg gagcagtcga acgagttctg gtgcgtggtc
     1201 gccgcacccc actggtacgg ctttccttgc tgggccaagg cgaactggat ggccgagccg
     1261 gccgcctggg tcggcgccgg cagcaggttg ccggtgccct cgccgcaccc ggtcgggtcc
     1321 tcgacggccc cgatgttgcc gatcaggtgg gcggccatcg cctcccagcg gtggtagcga
     1381 tcggggaacg ccgagcgctc cacgtcctgc gccgaatcgc cgggccgctg ctcctgccag
     1441 ttgggcaccc cgttgagcac gtcgtagaac ttgttgatcg cgtacaccgg gttggtgacc
     1501 tgtgccggcg taccccagcc catcgacggc cgcatctgga acatgccgag cgagtcgcgg
     1561 tcgccgtagt tgaggctgcg caggccggac tcggtcatgc cggcctggat cgcgatctgc
     1621 cacgcgagcg gcggcagctg gcggtccttg ccgatctgga tgatcagcgc gacgttctgg
     1681 cgctggtcct ccttcagcct ggccgcgtcg gccgcg
//
LOCUS       DATADN010000922         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_569294, whole genome shotgun sequence.
ACCESSION   DATADN010000922 DATADN010000000
VERSION     DATADN010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569294"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1566
                     /gene="soxC"
                     /locus_tag="VHH53_11975"
     CDS             283..1566
                     /gene="soxC"
                     /locus_tag="VHH53_11975"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEX2290882.1"
                     /translation="MSQNKSGVIDSRQSPRATTRRQVLVGAAGGVIAGSAAGRGAGAA
                     PLGVAEWSKEHGRPIEPAAYGLPSKFEAHVARRRTDVLVNRQNFSDWSMTPLQHQPGI
                     LTPNGLFFERHHNGTPDIDPRTHKLVIHGLVRQPLVFTMDDLLRYPSVSKFHFLECSG
                     NGLTDWTQARSKTVQQTHGLLSCTQWTGVPLSWLLDEAGLQPGAKWVALEGADGSAHT
                     RSIPIEKVMDDVLLVYAMGGEMLRPENGYPLRAFIPGWEGNVSVKWLRRIKVGDQPWH
                     FRSETARYTDPMPEGKWRQFSFTMECKSVITNPSGGMQLKGPGLYEIQGFAWSGNGKI
                     TAVDVTTDGGKSWQEAVLEEPVLDKCLTRFRYKWQWTGAPSKIASRAVDSTGYVQPTV
                     ADIAKVRALTGFVQHHNGVFPWAVSASGEVANAIA"
     gene            1583..2122
                     /locus_tag="VHH53_11980"
     CDS             1583..2122
                     /locus_tag="VHH53_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX2290883.1"
                     /translation="MALLAGSAVAGEKRPDVAYGKPISEADLAAWNIDIRTPDGTGLP
                     AGQGSVAQGKQVYEAKCLSCHGEDAKGGPMYPTMVGGIGSFTTNARVLTPGSMYPYAP
                     ILFDYIRRAMPMNEPQTLTNDEVYAVSAYLLNLNGLVPADAVLDAKSLTALKMPNRDG
                     FIPDDRPDVKATRCMKDCL"
     gene            complement(2088..>2389)
                     /locus_tag="VHH53_11985"
     CDS             complement(2088..>2389)
                     /locus_tag="VHH53_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290884.1"
                     /translation="RLHAVALADFRHCPCAFSENPGCECLSIDDLVIGSSDCIVHSDF
                     PPANRQYRHCLASDAASSFSDVEGRPRDIVESTGSSCLSVGAAASTSSLSCSELL"
BASE COUNT          462 a          705 c          844 g          378 t
ORIGIN      
        1 ctatcccggc cctgagcaca ccgcgtacat gactcctgcg gagctggagc gctttgcgaa
       61 gatggtcagc tggaccaacc cgccaatcga cgccgcgatc gagcgggaga aggcgaagca
      121 ggcggcaaag gctagagccg cgtcggattg agcatcgcat gccgtgagcg cgagcctgcc
      181 gcgcaggttc gccccattac taaatgagca agtgttgatg gctgcttgag atccgtttcg
      241 acgttcaccg cgaagtgaac gcaataacaa ttgggaggcg ccatgagcca gaacaagagc
      301 ggagtcattg actcacgaca atcgccgcga gcgacaacga gacgtcaagt cctcgtcggc
      361 gctgcgggcg gggtaatagc gggttcggcg gctgggcggg gggctggtgc ggcaccgctt
      421 ggggtggcgg agtggtcgaa ggagcatggg cggccgatcg agccggcggc gtatgggctg
      481 ccgtcgaagt tcgaggcgca tgtggcgcgg cggcgcaccg acgtgctggt gaaccggcag
      541 aacttctccg actggagcat gacgccgttg cagcaccagc cgggcattct gacccccaac
      601 gggctgttct tcgagcgcca ccacaacggc acgcccgaca tcgacccacg cacgcacaag
      661 ctggtgatcc acggcctggt gcgccagccg ctggtgttca ccatggacga cctgctgcgc
      721 tacccgtcgg tgtcgaaatt ccacttcctg gaatgttcgg gcaacgggct gaccgactgg
      781 acgcaggcgc gctcgaagac ggtgcagcag acgcacgggc tgctgtcgtg cacgcagtgg
      841 acgggggtgc cgctatcctg gctgctcgac gaggcggggc tgcagccggg cgccaaatgg
      901 gtggcgctgg aaggggcgga cggctcggcg cacacgcgct cgatccccat cgagaaggtg
      961 atggacgacg tgctgctggt ctatgccatg ggcggcgaga tgctcaggcc ggagaacggc
     1021 tatcctttgc gcgccttcat cccgggctgg gagggcaatg tctcggtgaa atggctgcgg
     1081 cggatcaagg tgggcgacca gccctggcac ttccgcagcg agacggcgcg ctacaccgac
     1141 ccgatgcctg agggcaagtg gcggcagttc agcttcacca tggaatgcaa gtcggtgatc
     1201 accaacccgt cgggcggcat gcagctcaag gggcctggcc tgtacgagat ccagggcttc
     1261 gcctggtcgg gcaacggcaa gatcacggcg gtggatgtga ccaccgatgg cggcaagagc
     1321 tggcaggagg ccgttctgga ggagccggtc ctggacaagt gcctgacccg gttccgctac
     1381 aaatggcagt ggacgggcgc accgagcaag atcgccagcc gggcggtgga cagcaccggc
     1441 tatgtgcagc ccaccgtggc ggacattgcc aaggtgcggg ccctgaccgg gttcgtgcag
     1501 caccacaacg gggtgttccc gtgggcggtg agtgcgagcg gggaggtggc caatgcgatc
     1561 gcttaagtac agcctcgcgg ccttggcgct gctggccggc agcgctgtgg cgggcgagaa
     1621 gcggcccgat gtggcctatg gcaagccgat cagcgaggcg gatctggcgg cgtggaacat
     1681 cgatatccgc actccggacg gcacggggct gccggctggc cagggcagtg ttgcgcaggg
     1741 caagcaggtc tacgaggcga aatgcctgtc ctgccatggc gaggacgcga agggcgggcc
     1801 gatgtacccg accatggtgg gcgggatcgg ctcgttcacg accaatgcgc gggtgctgac
     1861 gccgggcagc atgtacccct atgcgccgat cctgttcgac tacatccgcc gtgcgatgcc
     1921 gatgaacgag ccgcagacct tgaccaatga cgaggtctat gcggtgtcgg cctatctgct
     1981 gaacctgaac gggctggtgc cggccgatgc ggttctggat gccaagagcc tgaccgcact
     2041 caaaatgccc aaccgcgacg gcttcatccc agacgacagg ccagacgtca aagcaactcg
     2101 ctgcatgaaa gactgcttgt agaggccgct gccccgacag acaggcaact tgagccagtg
     2161 ctttcaacga tgtcgcgagg tcgtccttcg acatcggaga aggagcttgc cgcgtcgctg
     2221 gcaagacaat gtcgatattg acgatttgcc ggaggaaaat cggaatgcac gatgcagtcg
     2281 ctcgagccga tcacaaggtc atcaatggaa aggcattcgc agcccggatt ctcgctgaat
     2341 gcgcacgggc agtggcggaa atcggcgagc gccacggcgt gcaaccggg
//
LOCUS       DATADN010000923         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_254382, whole genome shotgun sequence.
ACCESSION   DATADN010000923 DATADN010000000
VERSION     DATADN010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254382"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VHH53_11990"
     CDS             complement(<1..753)
                     /locus_tag="VHH53_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2290885.1"
                     /translation="MPAAQEYSDEFRQRAVQMVFDGRSEAGGQPEAIERVADQLTVAR
                     QALRQWVEQAGAKETSESSDAAPPAEPDTFEQGLLLLQRGELDKAETLWRTAAEAGNT
                     DAATGLGRLLVQRGALVEAEAWWRSAAETGNIDAANYLGLLLVQRGELDEAASWWRST
                     AEAGNIDAGTMLGVLLMQRGELNEAETWLRSAAHAGNTIAAFSLGVLLRRRGDLDGAT
                     QWSRAAAEAGNADAANNLGLVLEERGELDEAVA"
     gene            complement(816..1229)
                     /locus_tag="VHH53_11995"
     CDS             complement(816..1229)
                     /locus_tag="VHH53_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290886.1"
                     /translation="MNDQQDRVSGCPYRELAVGWALHALEPAEESLVTRHVADCPTCA
                     SIVAETEEVGAMLALSVPQTIPSIGLQQRILSATGASRAAPVVSLAPPMQPARPITEP
                     SEVDFRGWLSITWADVVGHARVEQALLAAGNSRIA"
     gene            complement(1226..1543)
                     /locus_tag="VHH53_12000"
     CDS             complement(1226..1543)
                     /locus_tag="VHH53_12000"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX2290887.1"
                     /translation="MPEAGEALSPIPVPGADTVGMARVAAGQVRAALDRLPFEQRRVL
                     ALTYFDGHTQREIAVLTGVPLDTVKSRTLVAVQRLRSLLTDQLDPGALVAEAHMVREV
                     VDR"
BASE COUNT          256 a          636 c          542 g          279 t
ORIGIN      
        1 ggccacggcc tcatcgagtt cgccacgctc ttccagcacc aggccgaggt tgttggcggc
       61 gtcggcgtta ccggcttcgg ccgcggcccg tgaccactgt gtcgctccgt caagatcgcc
      121 gcgccgcctg agcagcacac cgaggctgaa cgcggcgatg gtgttgccgg cgtgggcggc
      181 gctgcgcaac caggtctcgg cctcattgag ctcgccgcgc tgcatcagca gcacgcccag
      241 catggtgccg gcgtctatgt tgccggcttc ggcggtgctg cgccaccagc tcgccgcctc
      301 gtccagctcg ccacgctgca ccagcagcag accgaggtag ttggcggcgt cgatgttccc
      361 cgtttcggct gcgctccgcc accacgcctc cgcctcgacg agggcgccgc gctgcaccag
      421 cagccggccc agaccggtgg cggcgtcggt gttaccggcc tcggcggccg tgcgccacaa
      481 cgtctctgcc ttatcgagtt caccgcgttg caacagcagc aagccctgct cgaaggtgtc
      541 gggttcggcg ggcggcgccg cgtccgagga ctcgctagtc tccttcgccc cggcctgctc
      601 gacccactgt cgcagggcct gccgggccac tgtcaactga tcagcgaccc gttcgatcgc
      661 ttcgggttgg ccacccgctt ccgagcgccc gtcgaacacc atctgcaccg cacgctgccg
      721 aaactcatca ctatattcct gggcggctgg catgatcagc gtctcccgag atatgagtct
      781 tacctgcact gtgaccgttc ggtatggccc gccgtctacg cgatcctgct gttacccgcc
      841 gccagcagcg cttgctcaac cctcgcgtga cccaccacgt cagcccaggt gatcgacagc
      901 cacccgcgga agtcgacctc agatggctcc gtgatgggtc gggctggctg catcggcggt
      961 gccagcgaca cgaccggcgc tgcccgactg gcacccgtgg cactgagtat ccgctgctgc
     1021 aacccgatac tggggatcgt ctgcggcacg gacagcgcca gcatggcgcc gacctcctcg
     1081 gtctccgcga caatgctggc gcaggttggg cagtcagcca cgtgcctggt gaccagggac
     1141 tcctcggccg gctccagggc gtgcaaggcc cagccgaccg ccaactcacg gtacgggcag
     1201 ccgctaacac ggtcctgctg gtcgttcatc ggtccaccac ctccctgacc atgtgggcct
     1261 ctgcgaccag ggcgccagga tccaactgat cggtcagcag cgagcgcagt cgctgcaccg
     1321 cgaccaacgt gcgggacttc accgtgtcca gcggaacacc agtcagcaca gcaatctcac
     1381 gctgagtgtg cccatcgaaa taggtcaaag cgagcacccg ccgctgctcg aacggtaggc
     1441 gatcaagcgc cgcgcgcacc tggccggccg cgacgcgtgc catccccacc gtatcggcgc
     1501 ccggtaccgg gattggcgac aaggcctcgc ccgcctccgg caccgcaacc acccgtcgcc
     1561 ggatgcgggc gaggacttgt cgccaacccc ggtaccggcc gccgatgcgg tggggatggc
     1621 gcgcgtcgcg gccggccagg tgcgcgcggc gcttgatcgc ctaccgtttg agcagcggcg
     1681 ggtgctggct ttgacctact tcggcggcca cac
//
LOCUS       DATADN010000924         1822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1475866, whole genome shotgun sequence.
ACCESSION   DATADN010000924 DATADN010000000
VERSION     DATADN010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475866"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1006
                     /locus_tag="VHH53_12005"
     CDS             83..1006
                     /locus_tag="VHH53_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2290888.1"
                     /translation="MGRYIARRLLLTIPVLVGASFLIFAMVYALPGDPIAALAGDRPL
                     SPAVIAQLREDFNLNDPLWLQYVKYVGDLLQGDFGTSFNGRPVLETIEQRLPVTVRLT
                     VAAISFEVVIGIVAGVLAGIRRNGFLDNLVLVSSTLVISIPILVLGFVAQFLFGLQLG
                     WFPISGISDGWYSYLLPGLVLAAGSIAYVARLTRTSVAENLRSDYVRTARAKGLPPRT
                     VIIRHTLRNSLIPVVTFIGADIGTLMGGAIVTESVFNLPGIGRAVFDSIRAQEGAVVV
                     GIVTLLVFFFVLFNLVVDVLYAVLDPRIRYD"
     gene            999..>1822
                     /locus_tag="VHH53_12010"
     CDS             999..>1822
                     /locus_tag="VHH53_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2290889.1"
                     /translation="MTDQQQADLRHDEVGAGTTTGLATPSVDVTSERQNSLWSDAWGS
                     LRRNPLFWLGALLCALFVLMAIVPQLFAGGADPRNCDLGNSLEPPSAQHWFGFNQQGC
                     DYLANVVYGARNSLLIGVLAVLFLLVLGVVVGAIAGFYGRATDSILSRLTDIFYALPL
                     ILGALVLLRVGPSTGLPVISDRGAGAVAIALALFSWMTAMRLVRSQVIAIKNSDYVAA
                     ARAMGASNSRILVRHILPNAVAPVLVYATITIGVLIAAEATLTYLGVGLQRPNISWG"
BASE COUNT          234 a          670 c          592 g          326 t
ORIGIN      
        1 ccggcggtct gtcgtgggtc ccagaatgaa cgacgcgacg ggacgccgaa gctgcgtccg
       61 cgtgtcctga ccgaggagcg acatgggccg ctacatcgcg cgccgcctcc tgctgaccat
      121 ccccgtcctc gtcggggcgt ccttcctcat cttcgccatg gtctatgccc tccccgggga
      181 ccccatcgcc gccctggccg gcgaccggcc gctcagcccc gcggtcatcg cccagctgcg
      241 cgaggacttc aacctcaacg acccgctgtg gctgcagtac gtgaagtacg tgggcgacct
      301 gctgcagggc gacttcggca cctccttcaa cggccgtccg gtgctcgaga ccatcgagca
      361 gcggctcccg gtcaccgtgc ggctgacggt cgcggccatc tcgttcgagg tggtcatcgg
      421 catcgtcgcg ggcgtgctcg ccggcatccg ccgcaacggc ttcctcgaca acctggtcct
      481 ggtgtccagc accctggtga tctcgatccc gatcctggtg ctcggcttcg tcgcgcagtt
      541 cctgttcggc ctgcagctgg gctggttccc gatctccggc atcagcgacg gctggtacag
      601 ctacctgctc ccgggcctgg tgctggccgc cggctcgatc gcctacgtgg cccggctgac
      661 ccggaccagc gtcgcggaga acctgcgcag cgactacgtc cgcaccgccc gggccaaggg
      721 cctcccgccc cgcacggtca tcatccggca cacgctgcgc aacagcctca tcccggtcgt
      781 gaccttcatc ggcgccgaca tcggcacgct gatgggcggg gcgatcgtca ccgagtcggt
      841 gttcaacctg ccgggcatcg gccgggcggt gttcgactcg atccgggctc aagagggggc
      901 ggtcgtcgtc ggcatcgtga ccctgctggt gttcttcttc gtcctcttca acctggtggt
      961 ggacgtgctc tacgccgtcc tcgacccgag gatccgctat gactgaccaa cagcaggccg
     1021 acctgcgcca cgacgaggtc ggggccggga cgacaaccgg cctggcgacg ccgtcggtag
     1081 acgtcaccag cgagcggcag aacagcctgt ggagtgacgc ctggggctcg ctcaggcgca
     1141 acccgctctt ctggctcggt gccctcctgt gcgccctctt cgtgctcatg gcgatcgtgc
     1201 cgcagctgtt cgctggcggc gccgacccgc gcaactgcga cctcggcaac tccctcgagc
     1261 cgccgtcggc gcagcactgg ttcggcttca accagcaggg ctgtgactac ctggccaacg
     1321 tggtgtacgg cgcgcgcaac tcgctgctga tcggcgtact cgcggtgctg ttcctgctcg
     1381 tgctgggtgt ggtcgtcggg gcgatcgccg gcttctacgg ccgggccacc gacagcatcc
     1441 tgtcccggct caccgacatc ttctacgcgc tgccgctcat cctcggggcg ctggtgctgc
     1501 tgcgggtcgg gccctccacc ggcctgccgg tgatcagtga ccgcggtgcc ggggcggtgg
     1561 ccatcgcgct ggcgctgttc agctggatga cggcgatgcg cctggtccgc tcgcaggtca
     1621 tcgcgatcaa gaactccgac tacgtggccg ccgcccgggc gatgggcgcc tccaacagcc
     1681 gcatcctggt caggcacatc ctgccgaacg ccgtggcgcc ggtcctcgtg tacgcgacca
     1741 tcaccattgg cgtgctgatc gccgccgagg cgaccctcac ctacctgggt gtcgggctgc
     1801 agcggcccaa catctcctgg gg
//
LOCUS       DATADN010000925         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1026307, whole genome shotgun sequence.
ACCESSION   DATADN010000925 DATADN010000000
VERSION     DATADN010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026307"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1563)
                     /locus_tag="VHH53_12015"
     CDS             complement(<1..>1563)
                     /locus_tag="VHH53_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX2290890.1"
                     /translation="FSVAYQLPNLIAALVLEATFTAIFVPVLARAERDDPDNGTAFVR
                     RLVTLVTTLLVLTTLLAVASAPLLVGLMLGGDPEVNRDLTTAFAYLLLPQVLFYGLSS
                     VFMAILNTRNVFGPPAWAPVCNNVVALATLALYLVVPGELSVDPVQMGNAKLLVLGVG
                     MTLGTVTQVLVLLVAIRKERISLRPLWGVDDRMKRFGSMAAAMVLYVLISQIGLIVGN
                     RIASGAAASGPAIYNYTWLVLQLPFGMIGVTVLTVVMPRLSRNAAANDQRAVLADLSL
                     ATRLTMVTLVPIVALMTVGGPAIGSALFAYGNFGEVDANYLGMAITLSAFTLIPYALV
                     LLQLRVFYARQQPWTPILLIVVITTVKIIASLIAPHLTDDPDLVAGYLGTANGLGFLA
                     GATVGYFLLRANLDPPNGRLINLQVIRTILVTIAASLLAGLLAHVVDQVLGLESLTRN
                     WGGAGSLLRLLVLGVIMVPIIAGVLLAARVPDAQAALAAVRRRLGRSATMPPRRAAAA
                     ARPRGRTPLTYPE"
BASE COUNT          294 a          516 c          550 g          203 t
ORIGIN      
        1 ttcagggtac gtgagggggg tgcgcccgcg tggccgggcg gcggcggcag cacgtctcgg
       61 gggcatcgtc gccgaacggc cgaggcggcg tcggaccgca gccagtgccg cttgggcgtc
      121 gggcacccgc gccgcgagca gcacccccgc gatgatcggc accatgatca cgccgagcac
      181 cagcaaccgc agcagcgacc cggcgccgcc ccagttgcgc gtcagcgact ccagcccgag
      241 cacttggtcg acgacatggg ccagcaagcc ggcgagcagc gaggcggcga tggtgaccag
      301 gatcgtgcgg atcacctgca ggttgatcag ccggccgttg ggcgggtcga gattggcccg
      361 cagcaggaag tagccgacgg tcgcgccggc caggaagccc aggccgttgg cggtgccgag
      421 atatccggcc accagatcgg gatcgtcggt gagatggggg gcgatgagcg aggcgatgat
      481 cttgacggtc gtgatcacca cgatcagcag gatcggcgtc cacggctgct ggcgggcgta
      541 gaagacccga agctgcagca gaaccagggc gtagggaatc aacgtgaacg ccgacagcgt
      601 gatcgccatg ccgaggtagt tggcgtcgac ctcgccgaag ttgccgtagg cgaacagcgc
      661 gctgccgatg gccggaccgc cgaccgtcat cagcgccaca atcgggacga gcgtcaccat
      721 ggtcagccgg gtggccagtg agaggtcggc gagcacggcg cgctgatcgt tggcggcggc
      781 gttacggctc aacctcggca tcacgacggt cagcaccgtc acgccgatca tgccgaacgg
      841 caactgcagc accagccacg tgtagttgta gatcgcgggc ccggaagccg cagcgccgct
      901 ggcgattcgg ttgccgacga tcaacccgat ctggctgatc agcacgtaga gcaccatcgc
      961 cgcggccatc gagccgaacc gcttcatccg gtcgtcgacg ccccacagcg gtcgcaggct
     1021 gatgcgctcc ttgcggatcg cgaccagcag caccagcacc tgggtcaccg tgcccagcgt
     1081 catgccgacg ccgagcacca acagcttggc attgcccatc tgcacggggt cgaccgagag
     1141 ttccccgggc acaaccagat acagcgcgag ggtggccaac gccaccacgt tgttgcagac
     1201 cggtgcccag gcaggcggcc cgaacacgtt gcgggtgttc aggatcgcca tgaacaccga
     1261 cgacagcccg tagaacagca cctgcggcag cagcaggtag gcgaaagccg ttgtcaggtc
     1321 gcggttgacc tccgggtcgc cgccgagcat caacccgacc agcagcggcg ccgacgccac
     1381 cgccagcagg gtggtcaaca ccagcagcgt ggtcaccagc gtcaccaggc ggcggacgaa
     1441 cgcggtgccg ttgtcggggt cgtcgcgctc ggcacgggcc agcacgggta cgaagatcgc
     1501 ggtgaaggtc gcctcgagca ccagtgcggc aatcaggttc ggcagttggt aggccacgct
     1561 gaa
//
LOCUS       DATADN010000926         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1026376, whole genome shotgun sequence.
ACCESSION   DATADN010000926 DATADN010000000
VERSION     DATADN010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026376"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /gene="pyk"
                     /locus_tag="VHH53_12020"
     CDS             complement(<1..1197)
                     /gene="pyk"
                     /locus_tag="VHH53_12020"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX2290891.1"
                     /translation="MSRRAKIVCTIGPATATPEKIRELVRAGMDVARLNFSHGTHDDH
                     ERVYTMVRQASDEYGKAVGILADLQGPKIRLGRFAAGPVEWRTGDLVRVTVEDVAGTH
                     DRVSTTYTGLAADAREGDALLVDDGKVALVVRKIEDSDVVCEVVEGGTVSDHKGLSLP
                     GMSVSVPALSAKDITDLEFALRLRVDLVALSFVRSPADIDMVHRTMDEVPGGRLPVIA
                     KLEKPEAVENLEAVVLAFDGMMVARGDLGVELPLELVPLVQKRAIQIARENAKPVIVA
                     TQMLDSMIGNARPTRAEASDVANAVLDGADAVMLSGETSVGRYPIQSVETMSRIVQAV
                     EMDSPNVPPLNHVPRTKRGVLSYAARDIGERLEAKALVAFTQSGDTVRRLARLHTHLP
                     LLAFTPE"
     gene            complement(1284..>1950)
                     /locus_tag="VHH53_12025"
     CDS             complement(1284..>1950)
                     /locus_tag="VHH53_12025"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX2290892.1"
                     /translation="ATDWEQYFGPGGNPMDALKIWQGGLGIWGAIALGSLGAWIGCRR
                     RGVPLPAMADAVAPAIVVAQAIGRLGNWFNQELYGGPTSLPWGLEIRERVNPATGALD
                     PLNGIAQGSPVQIVHPTFLYELLWCLGVAALVVWADRRFHLGHGRAFALYVAGYTLGR
                     FWIELMRTDPATMVFGQRINVITSVVVFLGAMLYLLLARRGREEPDTLHGRAPAQRAS
                     ADV"
BASE COUNT          346 a          682 c          612 g          310 t
ORIGIN      
        1 ctcaggggtg aacgccagca gcggcaagtg ggtgtgcaac ctagccagcc tgcggacggt
       61 gtcaccggac tgggtgaacg cgaccaacgc tttggcctcc agccgctccc cgatgtcgcg
      121 ggcggcgtag gacagcacac cccgcttggt ccgcggcacg tggttgagcg gtgggacgtt
      181 gggcgagtcc atctcaacgg cctgcacgat acggctcatg gtctcgacgg attggatcgg
      241 gtagcgaccg acgctggtct ccccagacag catgaccgcg tcggcgccgt cgagcaccgc
      301 gttggccacg tcggaggcct ccgccctggt tggccgggca ttgccgatca tggaatcaag
      361 catttgggtg gccactatga ccggtttggc gttctccctg gcgatctgga tggcccgctt
      421 ctgtaccagc ggcacaagtt ccaagggcag ctcgaccccg agatcgccac gagccaccat
      481 catcccgtcg aaggccagta cgaccgcctc gaggttctcc accgcctcag gcttctccag
      541 cttggcgatc accggcagcc ggccaccagg cacctcgtcc atcgtgcggt gcaccatatc
      601 gatatcggct ggggagcgca cgaaggacag cgctaccagg tccacccgca accgcagcgc
      661 gaactccaag tcggtgatgt ccttggcgga cagcgccggc accgagacac tcatccccgg
      721 cagagacagg cccttgtggt cgctgaccgt gccaccctcg acgacctcgc agaccacgtc
      781 ggagtcttcg atcttgcgga ccaccaaggc gaccttgccg tcatcaacga gcagcgcgtc
      841 accttcgcgg gcatcggcgg cgagccccgt ataggtggtg gagacccggt cgtgggtgcc
      901 ggcgacatcc tcaactgtga cccgcaccaa gtcgccggtg cgccattcca ccggtccggc
      961 cgcgaaccgt ccgagccgga tcttgggtcc ctggaggtca gcgaggatcc ccaccgcttt
     1021 gccgtactca tcgctggcct gccggaccat cgtgtagacc cgctcgtggt catcgtgcgt
     1081 gccgtggctg aagttgaggc gggcaacgtc catgccggcc cggaccaact cccggatctt
     1141 ttccggggtc gcggtagccg gaccgatcgt gcagacgatc ttcgctcgtc gactcacggt
     1201 gcccagcgta gcggtgcgta gtacgcgttc cgaagccgcc aggttgcgtg cgagtgacca
     1261 gccggacact cccgagctac cagctacacg tccgcagacg cacgctgagc cggggcgcga
     1321 ccgtggagcg tgtctggctc ctcccggcca cggcgggcca gcagcaggta gagcattgca
     1381 cccaggaaca ccacgacgga cgtgatcacg ttgatccgct gcccgaagac catcgtcgct
     1441 ggatcagtac gcatcagctc gatccagaac ctgcccaggg tgtaaccagc gacataaagc
     1501 gcgaaggccc gcccgtggcc gaggtggaaa cgccggtcgg cccagaccac gagcgccgcg
     1561 acgcccagac accacagcag ttcgtagagg aaggttggat gcacgatctg caccggggaa
     1621 ccctgcgcga tcccattcaa tggatcgagg gcaccggtgg ccgggtttac ccgctcgcgg
     1681 atctccagtc cccagggcag tgaagtcggg ccgccgtaga gctcctggtt gaaccaattg
     1741 cccagcctcc caatggcctg ggccaccacg atggccggcg cgactgcatc tgccatcgcc
     1801 ggcagcggga cgcctcggcg ccggcagccg atccaagccc ccagactgcc gagcgcgata
     1861 gcaccccaga tacctaagcc gccctgccag atcttcaggg cgtccatcgg gttaccaccg
     1921 ggcccgaagt actgctccca gtccgtcgct
//
LOCUS       DATADN010000927         1698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1026454, whole genome shotgun sequence.
ACCESSION   DATADN010000927 DATADN010000000
VERSION     DATADN010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026454"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1009
                     /locus_tag="VHH53_12030"
     CDS             293..1009
                     /locus_tag="VHH53_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HEX2290893.1"
                     /translation="MTKSKETSAVGTDPPKPLPGRHLAPRGERPNRWLAGLGVVSVLL
                     VTVLLVVMASGVGQEPRGRPAATASTADILGPPPPPPARPTAFGNLVSNWSFEQDLRG
                     WSAVGPATVSRELQGRTSGSSAAVRALGRQPQPVGLVLPNAVSTAQEGSRYVASAWVR
                     STPPGVKVTLRLGMMGGGSEASQAVTTTPPGDRWGRVTVVHTVAAAQSSLDLQIVAAG
                     VRPGEALFVDEVELREGRTP"
     gene            1047..>1698
                     /locus_tag="VHH53_12035"
     CDS             1047..>1698
                     /locus_tag="VHH53_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2290894.1"
                     /translation="MNGNADGRELNLLMEDPRQRVGRRGFLRVAALLGVAGATGAVTG
                     AGAGYLAGRAKQGDDNQDRAAAGVAGPRRREQTLNIYNWSDYIDEKTVPLFQAWTNIR
                     VNYDVYSSNEDLLAKLRAGPTNYDIIVPTNQFIPTYRRLGLLQPLRQELIPNLANLDK
                     AFVETDYDPGNKYTIPWQWGTTGLGYNTKRMPGGKLDSWTALYEPNPSLGGRLSILR"
BASE COUNT          287 a          566 c          574 g          271 t
ORIGIN      
        1 cgccgcagtc accttggcag cgaccgatag ggagtgggag gggggagtac tccaatcttt
       61 cgccaagctt tcgccgtacc gtcggcgctc cgaggcaact ggagttccca cgcattcatg
      121 ctgcacgcaa ccgtcgtatc cagctgtcgt tgacgaggac ccggccatgg ctccggttgc
      181 tcgttgagaa ccggccgcta ttggaggcca cccttccctg accgagatcc ccagcgggga
      241 tcaccccgat cagctacacg ggaacagctt ctcaatccaa aggtggcgcg gcatgacgaa
      301 gtcgaaggag acgtctgctg tcggcacgga cccgcccaag ccgttgcctg gccgccacct
      361 ggcccctcgg ggcgagcgtc caaatcggtg gctggccggc ctcggtgtcg tgtctgtcct
      421 gctggttacg gtgctgcttg tggtcatggc atctggcgtc gggcaggaac ccagggggcg
      481 gccggcagca actgcttcga ccgcggacat actcggtccg ccgccaccgc cgccggctcg
      541 gcccacggca tttggaaatc ttgtcagcaa ctggagtttt gagcaggacc tgcgtggatg
      601 gtcggcggtc ggcccggcta cggtcagccg cgagctgcag ggccggacct ccgggtcgtc
      661 ggcggcggtg cgggctctcg gtcgccaacc ccaaccagtc gggctggtct tgcccaacgc
      721 ggtgtcgacg gcacaggagg gcagccgcta tgtggcttcg gcctgggtgc gctcgacccc
      781 gccgggcgtc aaggtcaccc tgcggctggg gatgatgggc ggcggctcgg aggcttcgca
      841 ggccgtcacc acgaccccgc ctggggaccg ctggggccgg gtcaccgtgg tccacaccgt
      901 tgccgccgcc cagagcagtc tcgatcttca gatcgtggcc gcaggggtgc gtccgggcga
      961 ggcgttgttc gtggacgagg tcgaactgcg agaagggagg accccctgag cgcaccgtgg
     1021 gcctgcaagc acccgagggg agcgaggtga acggcaacgc tgatggcagg gagctcaacc
     1081 tgctgatgga ggacccccgc cagcgagtcg gccgacgagg gttcctgcgg gtcgcggcac
     1141 tgctgggggt cgccggagcg accggggcgg tgaccggagc aggagccgga tacctcgctg
     1201 gacgggccaa gcagggcgac gacaaccagg atcgcgcggc agcgggcgtg gccggtcccc
     1261 gtcggcggga gcagaccctc aacatctaca actggtcgga ctacatcgac gagaaaaccg
     1321 ttccgctgtt tcaggcctgg accaacatcc gggtcaatta tgacgtctat tcctccaacg
     1381 aggacctgct ggccaagctc cgggccggcc cgaccaacta cgacatcatc gtgccgacca
     1441 atcagttcat ccctacctac cggcgactgg gcctgttgca gccgctgcgc caggagctga
     1501 tccccaacct ggccaacctc gacaaggcgt ttgtggagac cgactacgac cccggcaaca
     1561 agtacacgat tccctggcag tggggcacga ccggcctggg ctacaacacc aaacgcatgc
     1621 ccggcggcaa gctcgactcc tggaccgcgc tgtatgagcc caaccctagt cttggcggcc
     1681 ggctctccat cctgcgtg
//
LOCUS       DATADN010000928         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_98420, whole genome shotgun sequence.
ACCESSION   DATADN010000928 DATADN010000000
VERSION     DATADN010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98420"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..868)
                     /locus_tag="VHH53_12040"
     CDS             complement(71..868)
                     /locus_tag="VHH53_12040"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX2290895.1"
                     /translation="MLLALDIGNTNIVLGLFDGGQGGTRASAMLVRDWRMHTDARMTA
                     DELALTVRGLLGEYSGKVTGIAALSTVPALLRELRLMLDRYWASLPRVIVEPGVRTGV
                     PLHYDNPREVGADRIVNTLAAYTLYGGPAIVVDFGTSTNFDVISARGEFLGGALAPGI
                     EISVDALAARAAQLVKVQLVRPRSVIGKSTVEALQSGLLFGFAGQVDGLVGRITAELA
                     PAADSEVVVVATGGLAPLLIAESKTITCHAPDLTLQGLRMVYERNIS"
     gene            complement(853..1326)
                     /gene="panD"
                     /locus_tag="VHH53_12045"
     CDS             complement(853..1326)
                     /gene="panD"
                     /locus_tag="VHH53_12045"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX2290896.1"
                     /translation="MFRTMLKSKIHRATVTQSDLHYVGSVTLDEDLMDAADLLAGERV
                     AIVDITNGARLETYVIPGARASGVVGINGAAAHLVAPGDLVILISYGTMDETTSRRYQ
                     PRVVFVDAGNRIVETGTDPAHAPANSDLIAGSAGGDLIAGSAGGDLIRGSECCSP"
     gene            complement(1326..2219)
                     /gene="panC"
                     /locus_tag="VHH53_12050"
     CDS             complement(1326..2219)
                     /gene="panC"
                     /locus_tag="VHH53_12050"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX2290897.1"
                     /translation="MIGSTSAGLAVHDDPTALTAVTRGLRRTGRPVVLVPTMGALHAG
                     HRELIRAARAIPAASTVVSIFINPLQFGSQQDLDCYPRQFDADLAMCQEEGVELVFAP
                     SAEAMSPPGSDTTVVPGALGAELEGASRPGHFTAVLTVVAKLFGLVGPDRALFGEKDY
                     QQLVLIKRMVTDLRLGVQVVGIPTVREADGLASSSRNVHLDAQARAAAVALPAALAAG
                     AHAGVQGADAVLTAARQVLAAQPAVALDYLALRDPWLGPAPQQGQARLLVAATIGTTR
                     LIDNVGLRLGATRNSNRRGGP"
     gene            complement(2216..3133)
                     /locus_tag="VHH53_12055"
     CDS             complement(2216..3133)
                     /locus_tag="VHH53_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX2290898.1"
                     /translation="MPRSGPFVSGDVIGRPARLAVGVVSAGRVGSVLGAALAGAGHVV
                     VGASAVSRVSLRRAEVLLPGVPVLAPDDVVRRCDLVLLAVPDDVLPGLVSGLAATGAF
                     RAGQIVVHTCGARGVDVLAPAIAAGVLPLALHPAMTFTGRAEDVARLRAACVGVTVAD
                     ETGWSVGEALVLEMGAEPVRVPSEARPLYHAALAHGANHLATLVRDAVDALEHAGVVP
                     AERLLGPLLEAALDNTLRHGDRALTGPVARGDLDTVRLHLRVLAESAPDLLDPYRAQA
                     ARTADRAEAAGLLEPAAAVQIRDMLREGR"
BASE COUNT          560 a         1155 c         1043 g          448 t
ORIGIN      
        1 gggtatcaga gcgcgacaca ccgggcaggg ggacggacac gccggggtgg aggtgaggac
       61 gggtaaggga tcagctgatg ttgcgttcgt agaccatccg caatccctgc agagtgagat
      121 cgggcgcgtg gcacgtaatg gtcttcgact cggcgatcag cagtggcgcc agacccccgg
      181 tggcgaccac caccacctcg ctgtcggccg ccggtgccag ctcagcggtg atccggccga
      241 ccagcccgtc gacctgcccg gcaaacccga acagcagccc ggactgcagg gcttctacgg
      301 tgctcttgcc gatcaccgat cgtggccgga cgagctgtac cttcaccaac tgagcggccc
      361 gcgcagcaag cgcatccacg gaaatctcga taccaggagc cagggcgcca ccgaggaact
      421 ccccacgtgc gctgatcacg tcgaagttcg tggaggtacc gaagtcaaca acgatcgccg
      481 ggccaccgta gagggtgtag gcagccaacg tgttgacgat ccgatcggcg cccacctcgc
      541 gcgggttgtc gtaatgcaat ggcaccccgg tacgcacccc gggctctacg atcacccggg
      601 gcagtgaggc ccagtaccgg tctagcatca accgcaattc gcgcaacaga gcgggcacag
      661 tggacagtgc cgcgatcccg gtgaccttcc cggagtactc gccgagcagc ccgcggaccg
      721 tcagcgccag ctcatcggcg gtcatcctgg cgtcggtatg cattcgccag tcgcgcacca
      781 gcatggcgct cgccctcgtg ccaccctgac cgccatcgaa caacccgagg acgatgttgg
      841 tgttgccaat gtctagggcg agcagcactc gctacccctg atcagatcac cgccggcgga
      901 gccggcaatc agatcaccgc cggcggagcc ggcaatcaga tcactgttcg ccggggcatg
      961 cgccgggtcg gtacccgttt ccacaatccg gttgccggcg tcgacgaata ccacccgtgg
     1021 ctggtaacgc cgcgacgtgg tctcgtccat cgtgccgtaa gagataagaa tcaccaggtc
     1081 tccgggcgcc accagatgcg cagcggcacc gttgatcccg accacgccgc tggcccgggc
     1141 accggggatg acataggtct ccagccgggc gccattggtg atgtccacga tcgctacccg
     1201 ctccccggcg agcaggtcgg cggcgtccat gaggtcctca tcgagggtca ccgacccgac
     1261 gtagtgcaga tccgactggg taacggtggc cctgtggatc ttggacttca gcatggtccg
     1321 gaacatcacg ggcctcctcg cctgttcgag ttgcgcgtcg cgccgaggcg caagccgacg
     1381 ttgtcgatca accgcgttgt cccgatggtg gcggccacca gcagccgggc ctgcccctgc
     1441 tgcggtgcgg gacccagcca cggatcgcgc agcgccaggt agtcgagcgc tactgcgggt
     1501 tgtgcggcca gcacctgcct ggccgcggtg agtaccgcat cggcaccttg cacgccggcg
     1561 tgggctccag ctgccaaggc cgcgggtagt gcaacagcgg cggcgcgcgc ctgcgcatcc
     1621 aagtgaacgt tgcgtgacga cgaagccaag ccgtccgctt cccgcaccgt gggtattccc
     1681 accacctgca cgcctaaccg caggtcggtc accatccgct tgatgagcac gagctgttga
     1741 tagtcctttt caccaaacaa tgcccggtcc ggaccgacga gtccgaagag cttggcgacc
     1801 actgtgagca ccgcagtgaa gtgaccaggc ctgctggcgc cctccagctc ggcacccaat
     1861 gctccgggca ccacggtggt gtcagacccc ggtggagaca tcgcctccgc gctgggcgcg
     1921 aacaccagct ccacaccctc ctcctggcac atcgcaagat cggcgtcgaa ctggcggggg
     1981 taacagtcca ggtcttgctg cgacccgaac tgcaaagggt tgatgaagat cgacaccacg
     2041 gtactggcgg ccgggatggc ccgggccgct cgaatcagct cccgatggcc tgcgtgcagc
     2101 gctcccatcg tcgggaccag taccaccggt cgccccgtcc ggcgtaaccc acgggtcacc
     2161 gcagtcaatg cagtggggtc gtcgtgcacg gccagcccag cagaggtgga tccgatcacc
     2221 gcccctcccg aagcatgtca cggatctgga cggcggccgc cggttccagc agtccagccg
     2281 cctcggcgcg gtcggcggtc cgggctgcct gtgcccggta gggatcgagc aggtccgggg
     2341 cggactccgc gagcacccgc agatgcagcc ggacggtgtc gaggtcgccc cgggctaccg
     2401 gcccggtcag ggcccggtca ccgtggcgca gggtgttgtc cagcgccgcc tccagcagcg
     2461 ggcccagtaa ccgctcggcg ggtaccacgc cggcatgctc cagcgcgtcc accgcgtcac
     2521 gcaccagcgt ggccagatgg ttggccccgt gcgccaaggc cgcgtggtaa agcggccggg
     2581 cttccgaagg gacgcgcacc ggctcggcgc ccatctccag caccagcgcc tcgcctacgc
     2641 tccagccggt ctcgtcggcg acggtgaccc caacacacgc agccctgagc ctcgcgacgt
     2701 cctcagcgcg cccggtgaag gtcatcgcgg gatgcagagc cagcgggagt acgccggcag
     2761 cgatcgccgg cgccaggacg tccaccccgc gcgcgccaca ggtgtgcacc acgatctggc
     2821 cggcccggaa cgccccggtg gcggcgagcc cggacaccag cccgggcagc acgtcgtcgg
     2881 gaacagccaa cagcacgaga tcgcaacgcc gtacgacatc gtcaggggcc agtaccggca
     2941 cgccgggcag caggacctcg gcgcggcgca aagatacccg ggacaccgcg ctggcgccga
     3001 ccaccacgtg gccggcgccg gctagagcag cgccgagcac cgaccccact cggccggcag
     3061 agaccacgcc caccgccaac cgagcggggc ggccgatcac gtccccggac acaaacggtc
     3121 ccgaccgcgg catcgtcgcc atcaccgtcc tccagacgtt ccagtcccgc cgttgctgcg
     3181 ggtaccggac ttccgtggcc gcaggc
//
LOCUS       DATADN010000929         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_716867, whole genome shotgun sequence.
ACCESSION   DATADN010000929 DATADN010000000
VERSION     DATADN010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716867"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..815
                     /locus_tag="VHH53_12060"
     CDS             537..815
                     /locus_tag="VHH53_12060"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290899.1"
                     /translation="MRRAAGIDVGMPLVVYVEDGRIVIETREQLANRIRRDVAGSLDR
                     CRIGGGRVDCRPPRRSGPRGPSVTTRGTPRCWIPLQCWRGRAACEWNR"
     gene            812..991
                     /locus_tag="VHH53_12065"
     CDS             812..991
                     /locus_tag="VHH53_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290900.1"
                     /translation="MSEADAVTAANLWTRTRAAGLSLGDRCCLALGARLALPTVTTDP
                     AWAGLDIDIPVRIIR"
     gene            1257..1499
                     /locus_tag="VHH53_12070"
     CDS             1257..1499
                     /locus_tag="VHH53_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290901.1"
                     /translation="MEEALRDRLIVRRHITWWQSPKHHLSDAEVAEVDWSRLHLLDRA
                     TLHSQAEQVVAAGPPRVIQFVVRAEALVLPPFADEP"
BASE COUNT          277 a          542 c          592 g          300 t
ORIGIN      
        1 cccggcgtga cgctgtcgaa ggccgacacg atcagcctgg ctcagtcgat tctgcagcgt
       61 gcccgatgag cgggggcgga agtcgcgcgg cgttgacggt cgactctgag gtcgacgcgc
      121 cgcttccggc gggtgtctcc cacctgctgg tggctaacga tgcgctgatc cccggccgct
      181 ggggtgtcca cgtcgcgctc tgcggcgcga ggagacgggc gcctgaaccc accgcgctcg
      241 agcacgagtg cgccgacgcc agctgcgact gtgttcacta ctgccccgac tgtgtgcagg
      301 aggccgctcg ttggagcgcc gaacccgtcg gacaggccgc ccgtgcgcct gaggccccgt
      361 aagccgtgaa gtcggggctg tcggtgctgg actagcgcag ccacgccatc gacgagcgcg
      421 ggcacgaagc cctcacctgt gtcagatata gtgtaaccgt gacacgttca tcgtcagagt
      481 ctccaggcgg ggaggttact gtcaatgagc aaggccgggt gacgatacca gcacaggtgc
      541 ggcgagctgc gggcatcgat gtcgggatgc cgctggtggt ctacgttgag gacgggcgca
      601 tcgtgatcga aacccgcgag cagctcgcta accgcatccg ccgtgacgtc gccggcagcc
      661 tggacaggtg caggatcggt ggtggacgag ttgattgccg accgccgcgc cgaagcggcc
      721 cgcgaggacc atcagtgacc acgcggggga cacctcggtg ctggattcct ctgcagtgct
      781 ggcgtggtcg cgcggcgtgc gagtggaacc gttgagcgag gccgacgcgg tcacggccgc
      841 gaatctctgg acgcgcacgc gcgcggcagg cctgtccctg ggtgatcgat gctgcctcgc
      901 gctcggggca cgtttggcac ttcccacggt gaccaccgac ccggcctggg ctgggctcga
      961 tatcgacatt cctgtgcgga tcattcgttg accgcttact gaggtgaaat cggtgatggc
     1021 ggttggtttg gtcatgccat ccctcagcac ctcatggagg caccgatgta cggttcggca
     1081 gtggtgactc tcagcctcaa cgttgtcgat aagcagcacg cgaggctgct ccagcacatc
     1141 gccgacgcct gggacctacg gcagacgatc cagattcctc acgcagtctg gccggatgcc
     1201 atcgtgcgtg gtgggcaggg tgtcatcgat gactccgaaa cggctgtcat cgcaccttgg
     1261 aagaggcgct gcgggaccgg ctcatcgtca gacgccacat cacgtggtgg cagtccccga
     1321 agcaccacct cagtgacgcc gaggtggccg aggttgactg gagccggttg catctgctcg
     1381 accgggccac tctccacagc caggccgagc aggtcgtggc ggcaggccct cccagggtta
     1441 tccaattcgt ggtgcgcgct gaggcgctgg tcctgccgcc cttcgcagat gagccatagg
     1501 ggtcggatgc cgtgctgcag ccgctgaaca actggatcgg cttcctattc gctagggaga
     1561 acgtcgaccg gacagttgca gtgctggccg catcccaggc gacgtcgatc actgcacggc
     1621 tcagcggaag gtctggccgg ggcactcctc ccggtactgc cagtcgtccg cgtgcgtctc
     1681 gatgaacgca cgtgcctgtg agcggccgag g
//
LOCUS       DATADN010000930         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1177276, whole genome shotgun sequence.
ACCESSION   DATADN010000930 DATADN010000000
VERSION     DATADN010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177276"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /gene="murJ"
                     /locus_tag="VHH53_12075"
     CDS             complement(<1..1545)
                     /gene="murJ"
                     /locus_tag="VHH53_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX2290902.1"
                     /translation="MAAAARTMAVATLVSRITGFLRLLGLAAVLGLGVINDSYTVSNT
                     LPAMLYELLIGGVLTSVLVPVLVRAAKEDADQGEAYAQRLVTIACATLGVATVLAIVG
                     APFLAVLFLGDSESADPQLATVFAYLLLPQILFYGLSGLFGAILQARSIFGPPAWAPV
                     LNNIIVLVFLGVYALVPGEITLDPARISEPKLLVLGLGTTAGIIVQALVQLPALRRAG
                     FHFGWRWGWDPRLTQASGMAAWFVLYVLVGQLGFVVTSRSAAQGEPGGVVIYSLVWLL
                     LQLPYGVLGYSLLTAIMPRMSRAAADADWRGVVKDLSLGSRYLTVLMLPITVIFTLAG
                     QQIGIALFSLGKAGSDAERLGAALAVSAFGLLPFAVTMLQLRVFYAMADARTPTMINA
                     LMVAVKLPLLLACPVLLDDADVVLGLTAVNSLSCVLGAMVGQAWLRRRLGRVETKRTL
                     ITLRNCLLAAVPAVGAVLGLRAVLDLDGGSGAVWGAWLDLVLTTVVVGVVMGGALIAL
                     RTPELTE"
     gene            complement(1620..>2123)
                     /locus_tag="VHH53_12080"
     CDS             complement(1620..>2123)
                     /locus_tag="VHH53_12080"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEX2290903.1"
                     /translation="LLRAVSSAWRGQPSRAMQVTQQERDRLDELRRSVRIVQSPGPYI
                     LAASDSPLLITVSNELPVGVQVQLSLSETAGLRTGAVGLQLVPARSTRQFVIPAEVSR
                     AGQFSVEARLSTPGGTPLGDPSTLQVSSTAFGTVTVALTVGAAAALVLLVVRGIVRRV
                     RRAREAG"
BASE COUNT          394 a          757 c          696 g          276 t
ORIGIN      
        1 ctcggtgagc tccggtgtgc gcagtgcgat cagggcacca cccatcacca cgccgacgac
       61 gaccgtggtg agcaccaggt ccagccatgc tccccagaca gcgccactgc cgccatcgag
      121 gtcgagtaca gcacgcaacc cgagcaccgc gcccaccgcc ggcaccgccg ccagcaggca
      181 attccgcagg gtgatcaggg tgcgcttggt ttccacccgg cccagtcgcc ggcgtagcca
      241 ggcctgcccg accatcgcac ccagcacgca cgacagcgag ttgaccgcgg tgagtccgag
      301 cacgacatcg gcgtcgtcga gcagcaccgg gcaggccagc aacagcggca gcttcaccgc
      361 caccatcaac gcgttgatca tggttggggt gcgggcatcg gccatcgcgt agaaaacccg
      421 caactgcagc atcgtgaccg caaacggcag caggccgaag gctgagaccg ccagcgcggc
      481 gccgaggcgc tcggcatcgc tgcccgcctt gcccagtgag aacagcgcga tcccgatctg
      541 ctgcccggcg agggtgaaga tcacggtgat cgggagcatc agcaccgtga ggtagcggct
      601 gcccagcgag aggtccttga ccactccgcg ccagtcggcg tctgccgctg cccggctcat
      661 ccgcggcatg atcgcggtga gcaatgagta gcccagcacg ccatagggta gttgcagcag
      721 caaccacacc aggctgtaga tcaccactcc acccggttcg ccctgcgccg ccgaccgcga
      781 ggtcaccacg aagccgagct ggccgaccag cacgtagagc acgaaccagg ccgccatccc
      841 gctggcctgg gtcagccggg gatcccaacc ccatcgccag ccgaagtgaa aacccgctcg
      901 acgcagcgcg ggcagctgca ccagcgcttg gacgatgatg cctgcggtgg tgcccaggcc
      961 gagaaccagc agcttcggct cgctgatccg tgctgggtcg agggtgatct cacctggtac
     1021 cagggcgtag acgcccagaa ataccagcac gatgatgttg ttgagcaccg gtgcccaggc
     1081 cggcgggccg aagatgctgc gggcttggag gatcgccccg aacagcccgg acaagccgta
     1141 gaacaggatc tgcggcagca gcaggtaggc gaacaccgtg gccagttgcg ggtcggcgct
     1201 ttcgctgtcg ccgagaaaca gcacggcgag aaacggcgcc ccgacgatgg cgagcaccgt
     1261 ggccacaccg agcgtcgcgc aagcgatggt gaccagccgt tgcgcgtacg cctcgccctg
     1321 gtcagcgtcc tccttcgccg cgcgcaccag caccggcacc aaaacgctgg tcagcacccc
     1381 gccgattaag agctcgtaga gcatcgccgg cagcgtgttc gagacggtgt aggagtcgtt
     1441 gatcacaccc aacccgagca cggcggcgag tccgagcagg cggaggaacc cggtgatccg
     1501 gctgaccagg gtggccaccg ccatcgtgcg ggcggctgcg gccaccgacg tctgcgaacc
     1561 acactgcggc ccggacccct tagccgcagt gtggttcgcg gtgtccgagg attccactgt
     1621 cacccggctt cccgtgctct gcgcacgcgg cgcacgattc cgcggaccac cagcagcacc
     1681 agcgcagcag cggcgcccac ggtgagcgcg acagtaacgg tgccgaaagc ggtggagctc
     1741 acctgcagcg tgctcgggtc gcccagcggg gtgccaccgg gggtgctcag ccgggcctca
     1801 acgctgaact gaccagctcg gctcacctcc gccgggatga cgaactgccg ggtgctgcgg
     1861 gccggcacca gctgcagccc gaccgccccg gtgcgcagcc cggctgtctc ggacaagctc
     1921 agctggacct gcacaccgac cggcaactcg ttgctcacgg taatgagcag tggggagtct
     1981 gacgccgcca ggatgtaagg acccggtgac tgcacgatcc gtaccgaccg gcgcagctcg
     2041 tcgagccggt cacgctcctg ttgggtcacc tgcatggccc ggctcggctg gccgcgccag
     2101 gccgacgaca ccgcacgcag cag
//
LOCUS       DATADN010000931         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_570414, whole genome shotgun sequence.
ACCESSION   DATADN010000931 DATADN010000000
VERSION     DATADN010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570414"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VHH53_12085"
     CDS             complement(<1..355)
                     /locus_tag="VHH53_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2290904.1"
                     /translation="MAIDGKSTDPAFDLTGRAAVVSGASRGIGHAIAAELLARGAAVA
                     ITARKPDELSTAADELAATVVGGADRVLALPGNAGDVTARADAVDRTVERFGSLDVLV
                     NNVGINPVYGPLMQVE"
     gene            494..>1972
                     /locus_tag="VHH53_12090"
     CDS             494..>1972
                     /locus_tag="VHH53_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2290905.1"
                     /translation="MITATDLELRAGSRILLSGATLRVQPGDRVGLVGRNGAGKTSTL
                     RVLAGESQPYGGQVRRAGSLGYLPQDPREGDLSVPARDRVLSARGLDELLHEMEKSQS
                     EMAELVDGHEQGRAVRRYSNLEDRFSALGGYAAESEAARICASLGLPDRVLAQPLRTL
                     SGGQRRRVELARILFGASDGSGGRSGTTLLLDEPTNHLDADSVTWLRDFLTAHDGGLV
                     VISHDTDLLAAVVNKVWVLDAVRSEADVYNLGWQRYLEARAADEKRRRRERANAEKKA
                     TALMAQADRMRAKATKAVAAQNMQHRATRLLAGAETEQRAERVARIRFPQPAACGRTP
                     LTASGLGKSYGSLEVFSGVDLAVDRGSRVVILGLNGAGKTTLLRLLGGMETPDAGQVE
                     AGHGLRVGYFAQEHDTLDLGATVWENLRHAAPDAPEQQLRTLLGAFLFCGEQLDQLAA
                     TLSGGEKTRLALAALVSSAANVLLLDEPTNNLDPVSRERVLDA"
BASE COUNT          294 a          671 c          705 g          302 t
ORIGIN      
        1 gttcgacctg catcaacggc ccgtacaccg ggttgatccc gacgttgttg accaggacgt
       61 ccagcgatcc aaagcgctcg actgtccggt ccaccgcgtc ggcccgggca gtcacatcac
      121 ctgcgttgcc gggtagtgca agtacccgat cggctccgcc cacgaccgtt gcggcgagtt
      181 cgtcggcagc ggtgctgagc tcgtcgggtt tgcgcgcggt gatcgccacg gcggcgccgc
      241 gggccagcag ctcggcggcg atcgcatgtc cgataccgcg gctggcgccg gaaaccacag
      301 cagcgcgtcc agtgagatca aaggcgggat cggtgctctt gccgtcgatt gccatggcgg
      361 caccgtaatc gggatgccgt cgccggtagg ataggtgtgt ggtcgcgaca agctgatgtg
      421 ctgccggccc cgcgcgctgc ggcgtgtggg gagttggacc cctgccggcg accacctttg
      481 gagccacctc atcgtgatca cagccaccga ccttgagctg cgtgccgggt cccgcatcct
      541 gctgtccgga gccaccctgc gggtgcagcc cggcgaccgg gtcggcctcg tcggtcgcaa
      601 cggtgccggc aagaccagca cgctgcgggt actcgccggt gagagccagc cctacggggg
      661 ccaggtccgc cgcgctggga gcctgggcta cctgccacag gatccccgtg aaggtgacct
      721 gtcggtgccg gccagggacc gggtgctctc ggcccgcggg cttgacgagc tgctgcacga
      781 gatggagaaa tcgcaatccg agatggcgga gctggtcgac ggccacgagc agggccgcgc
      841 ggtgcgccgc tacagcaacc tcgaggaccg gttctcggcg ctgggtggct atgccgccga
      901 gagcgaggcc gcgcggatct gcgccagcct cggcttgccc gaccgggtac tggcccagcc
      961 gctgcgcacg ctgtccggcg ggcagcgtcg ccgggtggag ctggcccgga tcctcttcgg
     1021 cgcctcggac ggttcggggg gccgctcggg caccacgctg ctgctcgacg agccgaccaa
     1081 ccacctcgat gccgattcgg tcacctggtt gcgcgacttc ctgacggctc acgatggcgg
     1141 cctcgtcgtg atcagccacg acaccgacct gctcgctgcc gtggtcaaca aggtctgggt
     1201 tctcgacgcg gtccgcagcg aggccgatgt ctacaacctg ggctggcagc gttacctgga
     1261 ggctcgggcc gcagacgaga agcggcgccg ccgggaacgg gccaacgcgg agaagaaggc
     1321 cactgccctg atggcgcagg ccgatcggat gcgggccaag gcgaccaagg ccgtcgccgc
     1381 ccagaacatg cagcaccgag cgaccagatt gctggccggc gccgagaccg agcaacgtgc
     1441 cgaacgggtc gcccggatcc gcttcccgca gccggccgcc tgcgggcgca ccccgctgac
     1501 cgccagtggg ctgggcaagt cctacggctc gctggaggtg ttcagtgggg tggatctcgc
     1561 ggtcgatcgg ggcagccggg tggtgatcct cggcctcaac ggcgccggta aaaccaccct
     1621 gcttcggttg ctcgggggga tggagacccc ggatgccggg caggtcgaag ccggccatgg
     1681 cctgcgggtg ggctacttcg cccaggaaca cgacacgctg gatctcggtg ccacggtgtg
     1741 ggaaaacctc cggcatgccg cgccggacgc tcctgaacag cagctacgca ccctgctcgg
     1801 cgccttcctg ttttgtggtg agcaactgga ccagttggcc gcaacgctgt ccggcgggga
     1861 gaagacccgg ctggcgttgg cggcgctggt gtcctccgcc gccaacgtgt tgttgctcga
     1921 cgagcccacc aacaacctcg acccggtctc gcgggagcgg gtgctcgacg cg
//
LOCUS       DATADN010000932         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_424535, whole genome shotgun sequence.
ACCESSION   DATADN010000932 DATADN010000000
VERSION     DATADN010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424535"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VHH53_12095"
     CDS             complement(<1..104)
                     /locus_tag="VHH53_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HEX2290906.1"
                     /translation="MPEGHTTHRLARRHQQCYGGSPVAVSSPQGRFASA"
     gene            complement(112..585)
                     /locus_tag="VHH53_12100"
     CDS             complement(112..585)
                     /locus_tag="VHH53_12100"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEX2290907.1"
                     /translation="MRVYLASDHAGFELKNFLVKHLTSRGHDVVDVGPEAFDADDDYP
                     PFCIEAARRVVGDAGSLGVVIGGSGNGEQISANKVSGCRAALAWSTDTAKLAREHNDA
                     QVAGIGARMHSPAEAAEIVETFLNTSFSAGERHSRRLRQISDFESTGEPPPMSRH"
     gene            635..1369
                     /locus_tag="VHH53_12105"
     CDS             635..1369
                     /locus_tag="VHH53_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEX2290908.1"
                     /translation="MRREHIQDGYDVRLEWGREGVALLGARCAVVVIVDVLSFCTAVD
                     VAVERGATVLPLPWGDERALDAAQRVGAILAGPRGSQGFSLSPTSLHTLPAGTRLALA
                     SPNGATLCAIAQRTGAHVLTGCLRNAAAIARTAHRLARGGPIGLVPAGERWGLPGEPM
                     RPALEDAVGAGAIADVLPGAFSPEAELTAAGFNAARSRGLHGVLAASASGRELATDGF
                     AADVTLAAAYNASETAPQLQGGVLTA"
     gene            1409..>1842
                     /locus_tag="VHH53_12110"
     CDS             1409..>1842
                     /locus_tag="VHH53_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2290909.1"
                     /translation="MTARWVELADGVLVRRYAELDLSVGLLIGDRSCLVVDTRSDPAQ
                     GAELHAAIREVTRLPCSVVVTHAHFDHCLGTAAFLPAPVWAHPRCRDDLARCGAAQHA
                     EALAWGRAHRALVRPVAPDHLVAKQAEVELGGRQVLLTYAGRG"
BASE COUNT          264 a          614 c          664 g          300 t
ORIGIN      
        1 gcgctggcga agcgaccctg tgggctggac actgccaccg gcgatccgcc gtaacattgc
       61 tgatgccgcc gagccaaccg gtgcgtcgta tgaccctccg gcatcgccgg ctcagtgcct
      121 ggacatcgga ggcggctccc cggtggactc gaagtccgag atctgtctga gccggcggga
      181 gtgccgctcg cctgcagaga atgatgtgtt gaggaaagtc tcgacgatct cggctgcttc
      241 ggcggggctg tgcatccggg cgccgatgcc cgccacctga gcatcgttgt gctcgcgggc
      301 cagcttggcc gtgtcggtgc tccaggctag cgcggcgcgg catcccgaaa ctttgttggc
      361 ggagatctgc tcgccgttgc ccgacccgcc aatcaccaca ccgaggctgc ctgcatcacc
      421 caccacccgg cgggccgcct cgatacagaa cggcggatag tcgtcgtctg cgtcaaaggc
      481 ctcaggaccg acgtccacca cgtcgtgccc gcgactggtc aggtgcttca ccaggaaatt
      541 tttgagctcg aagccagcgt gatcggaagc gaggtaaacg cgcacagtgt gcaacattgc
      601 catatcgcgg atcaccgggt gaggagggca cagtttgcgc cgggagcaca tccaggatgg
      661 ctacgacgtc cggctggaat ggggtcggga aggggtagcc ctgctcggag cacgctgcgc
      721 ggtggtggtg atcgtggatg tgctctcgtt ctgcacggcc gtggacgtag ccgtcgagcg
      781 cggggccacc gtactaccgc tgccctgggg tgacgagcgc gccctcgacg ccgcccagcg
      841 tgttggagcg atactggccg gtccccgcgg ttcgcagggt ttttcgctgt cgccgacctc
      901 gctgcacacc ctgccagccg ggacgcggct ggcactggcc tcccccaatg gcgcgacgct
      961 gtgtgcgatc gcgcagcgta ccggcgccca cgtgctcacc gggtgccttc gcaacgcggc
     1021 cgcgatcgcc cgcaccgcgc atcggctggc tcgcggcggt ccaatcgggc tggtgccggc
     1081 tggcgagcgg tgggggctgc ctggtgaacc gatgcggcca gctctcgagg acgccgtcgg
     1141 agcgggcgcg atcgctgacg tgctgcccgg ggcgttctca ccggaggcgg aactgaccgc
     1201 ggccgggttc aacgcggcac gctcgcgcgg tctgcacggt gtcctcgcgg ccagcgcctc
     1261 cgggcgggaa ctggccaccg acggcttcgc cgccgatgtc accttggccg cggcctacaa
     1321 cgccagcgaa accgcaccgc aactgcaagg cggagtgctg accgcatgac actcggggag
     1381 ggggcgtggc agggtttgag gagcgtgggt gacagcgcgg tgggttgagt tggccgacgg
     1441 ggtgctggtg cggcggtacg ccgaactgga tctgtcggtg gggctgctga tcggggaccg
     1501 gagctgcctg gtcgtggaca cccgcagcga tccggcacag ggcgctgagc tgcacgccgc
     1561 gatccgcgaa gtcacccgct tgccttgcag cgtggtggtc acccacgcgc atttcgatca
     1621 ctgcctgggc accgcggcct tcctgccggc cccggtgtgg gcccaccctc ggtgccggga
     1681 cgacctcgcc aggtgcggcg cggcccagca cgccgaggca ttggcttggg gtcgcgcaca
     1741 ccgcgcgctg gtgcgccctg tggcgcccga tcacctggtc gccaagcaag ccgaagtcga
     1801 actcggtggc cggcaagtgc tgttgaccta tgccggacgt gg
//
LOCUS       DATADN010000933         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_717411, whole genome shotgun sequence.
ACCESSION   DATADN010000933 DATADN010000000
VERSION     DATADN010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717411"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VHH53_12115"
     CDS             complement(<1..278)
                     /locus_tag="VHH53_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098921.1"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX2290910.1"
                     /translation="MAVLPVGDPAPPDGILPAAALRGLPDRPFGVYVHVPFCATRCGY
                     CDFNTYTPGELALSPSTWMDGLRRELDLAATVLETPLPADTVFVGGGTP"
     gene            519..1265
                     /locus_tag="VHH53_12120"
     CDS             519..1265
                     /locus_tag="VHH53_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2290911.1"
                     /translation="MTTELVHLVVQSGIATVTLDSPANRNALSVQLQAELHDALERSA
                     GDDSVRVVVLDHTGPVFCAGMDLTEPRDAVNELPRILERIWTHPKPVVAKLAGHARAG
                     GIGLTAAADIAIAADDVTFAFSEVRLGVVPAVISLTVLPRLSPHAAHELLLTGEAFDA
                     SRAVAVGLLNSAVPAEQLDAEVDRYTGMLVKGGPEALTMTKQLLRAPTRSQDFAAMLE
                     LSAARFASAEGQEGIRAFAEKRPASWIPPS"
     gene            complement(1266..1610)
                     /locus_tag="VHH53_12125"
     CDS             complement(1266..1610)
                     /locus_tag="VHH53_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX2290912.1"
                     /translation="MLHYVPDAARALAEARGRGALAHLMGPVTSKAGALDAIGAVLSF
                     PDWYGRNLDALYDCLVDLSWQPHGEHVLVWAGHRQLEAADPEGYRAVLAVLDDAATAG
                     AGCPLSVVLADA"
     gene            complement(1616..2047)
                     /locus_tag="VHH53_12130"
     CDS             complement(1616..2047)
                     /locus_tag="VHH53_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX2290913.1"
                     /translation="MSHSSTPSHQRISAALIGLLVLVTGVWLGRQALSSPPSGDRPAA
                     VTSAVDLPVQALSQLPPQASEVWQLIQHGGPFPYRQDGTVFDNREGRLPKQRSGFYRE
                     YTVPTPGESDRGPRRLITGGAAELYYTGDHYVSFVVVDPRR"
BASE COUNT          367 a          755 c          758 g          312 t
ORIGIN      
        1 ggggtgccgc cgccgacgaa caccgtgtca gccggcaagg gcgtctccag caccgtggcc
       61 gccaagtcca gctctcggcg cagcccgtcc atccaggtgg acggtgacaa agccagctca
      121 cccggggtgt aggtgttgaa atcgcagtaa ccgcagcggg tggcgcagaa cggcacgtgc
      181 acgtacacgc cgaatggtcg gtccggcaac ccgcgcaggg cagcagcggg caggattccg
      241 tccggcgggg cggggtcacc gacgggtagg acagccacgt gcgcattgtc cccacatccg
      301 aacgcgggtg gttcgcgctg ggggatagtg cgagctggtg aggcgccggc tgggggctgc
      361 agtccgggtc gctcacggag cagacgcgcg ctggttgctc gctgttacgg cacggggggc
      421 gaggtgcccc ggctgaaaag cacgggtggc acctgggtct cgcgcagcgc agcggagcac
      481 cccgtatcgg cttgtccccc cgcactacgg tcggccccat gaccaccgaa ctggtgcacc
      541 tggtcgtgca gtccgggatc gccaccgtca cactggactc ccccgccaac cgcaacgcgc
      601 tgtccgtgca gctgcaagcc gagctgcacg acgcgctgga gcgcagcgcc ggcgacgact
      661 cggtacgggt ggtggtgctc gaccacaccg ggccggtgtt ctgcgccggg atggacctca
      721 ctgagccccg ggatgccgtc aacgaacttc cccgcatcct ggaacggatc tggacgcacc
      781 cgaagcctgt ggtcgccaag ctggctgggc acgcccgcgc cggtgggatc ggcctgacag
      841 cagccgccga catcgccatc gccgcagacg acgtcacctt cgcgttcagc gaggtgcgac
      901 tcggtgtggt gcccgcggtg atctcgctga ccgtgctgcc gcggctgtca ccgcacgcgg
      961 cacacgagct gttgctcacc ggggaagcct tcgacgccag ccgggcggtc gccgtcggtc
     1021 tgctcaacag cgcggtaccg gccgagcagc tcgacgccga ggtggaccgc tacaccggga
     1081 tgctggtcaa gggtggccct gaagcactga ccatgaccaa gcaactgctc cgcgcgccga
     1141 cgaggagcca ggacttcgcg gcgatgcttg agctgtccgc cgcgcgattc gcctccgcag
     1201 agggccaaga aggcatccgc gccttcgccg agaagcgccc ggcatcctgg attccgccca
     1261 gctgatcacg cgtcagcgag aaccacggac agcgggcacc ccgcgccggc cgtggccgca
     1321 tcgtcgagca ccgcgagcac cgcccggtat ccctcgggat cggcagcctc gagctgccgg
     1381 tggccggccc agaccagcac gtgctcaccg tgaggttgcc aggacaggtc aacgaggcag
     1441 tcgtacagcg catcgaggtt gcggccgtac caatccggaa agctcaaaac ggcgccgatc
     1501 gcatccagcg cgccggcctt gctggtcacc gggcccatca ggtgtgccaa ggcaccccgt
     1561 ccccgggctt cagcaagcgc acgagccgca tccgggacat agtgcaacac ccgtctcacc
     1621 gccgagggtc gaccacgacg aaagacacgt agtggtcacc ggtgtagtag agctcggcgg
     1681 caccaccggt gatcaggcgg cgagggcccc gatcggactc gcccggtgtc gggacggtgt
     1741 actcccggta gaagccggac cgctgcttgg gcaaccgccc ctcccgattg tcgaataccg
     1801 tcccgtcctg ccggtaaggg aacggaccgc cgtgctggat cagctgccac acctcgctgg
     1861 cctgtggcgg tagctgcgac agagcctgta ccggcaggtc aaccgcggag gtgaccgccg
     1921 ccggacggtc accgctgggt gggctggaca gcgcctggcg gcccaaccag acaccggtta
     1981 ccagaacaag caagccgatc agtgcagcac tgatccgctg gtgggatggg gtggagctgt
     2041 ggctcacccg gctggcgctc ccgctgcaag tggccattct catggtggtg ctgctaccgg
     2101 tttgctgggg gacggctcgg gtcatcgatc gggtattcgg cctggtcttc gaacggctcg
     2161 accaccgcga cgcaaaggat gcgcaggcgc cg
//
LOCUS       DATADN010000934         3429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1178094, whole genome shotgun sequence.
ACCESSION   DATADN010000934 DATADN010000000
VERSION     DATADN010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178094"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VHH53_12135"
     CDS             <1..1504
                     /locus_tag="VHH53_12135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX2290914.1"
                     /translation="KGVLVHADGTVVATATRPHEVSMPHPGWVEHDPQAVWWADFAAI
                     SKELTAAAPEPVAAVGISGIGPCLLPVDSEDRPLRPAILYGVDTRATVEIDELTQALG
                     KQTILERCGSVLTSQAVGPKLAWLRANEPENWTATRRFLMASSFIVRKLTGSYILDHH
                     SASQCTPMYDPVEHRWIDEWTELIAPGLELPQLHWPSDVVGEVRADAAELTGIPAGTP
                     VVAGTIDAWAEATSVGVTAPGDVMVMYGTTMFLVEVLDRPRRSPDLWGTVGTFPGTHN
                     FAAGMATSGAITEWIRKLTGETSFDPLVAEAQQSPPGAQGLLMLPYFAGERTPLFDPD
                     ARGVLIGLTLRHNRGDIYRAALEATAYGVRHNLQAMRDAGGQARRLVAVGGGTKGGLW
                     TQIVSDVLDAPQQLPRQTIGACLGDALLAAIGIGHIKSGESPDTQIDMAEIGMAQWNP
                     IAATIEPRPEPAATYARLYDLYRELYPNTREIAHALAREQRVLSPRIGGS"
     gene            1504..2919
                     /locus_tag="VHH53_12140"
     CDS             1504..2919
                     /locus_tag="VHH53_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEX2290915.1"
                     /translation="MVASSSRSRVTTRRVYALGALAALLFGYDNGIIGAALLFIPQEL
                     PLSPLQTGAVVSATVLGAMVGALGSGPAADRLGRQRVLLVAGVVFTVGALGTGLAATV
                     NGLVGFRFILGLAIGIASVTVPLYLAEMAPASDRGTVTSLNQYLIIVGTALSAAIGYA
                     LAFTGSWRWMLLIGVFPAIVLMVGMFTMPDTPRSLVRRGHPEQARDVLVGLRADPDLA
                     ERELTEITALEQQQRDTRTGARLSAPWVRRLLLLGALLAIFQQITGINAIVYYAPTIL
                     TGFGVSETTALLFGFLNGLVNIATIALVVRSKIIDRRGRKPVLLAGLVGMAASLFAVG
                     AAVLILPVNSTALFVIAAAAFVVFTNAFSATWGPVLWVMLAEIFPLAIRGVAMAIATL
                     FNWLAAFVISLTFPSFVAFAGIGSAFIVFAVIGVVLFPVVWWMVPETKERSLESLETA
                     FRNGSRILPAERAAAETGGMA"
     gene            2916..>3429
                     /locus_tag="VHH53_12145"
     CDS             2916..>3429
                     /locus_tag="VHH53_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEX2290916.1"
                     /translation="MTVNYGTYGSSLRDLPRLRSTRRRRASSWDRTGGNDDRITLQPG
                     QTATLADIDAAGTINHIWVTVANERMLRDPWMRQPDYLRRLVLRAYWDGEEHPSIAVP
                     LGDFFGVGHGRTTNFVSAPLQMSPEDGKGFNCFFHMPFGAGARLEIASELTDELVYFY
                     YYIDYEELDEL"
BASE COUNT          540 a         1137 c         1133 g          619 t
ORIGIN      
        1 caaaggcgtg ctggtccacg ccgacggtac cgttgtcgcg acggcgaccc ggcctcacga
       61 ggtgtcgatg ccacaccccg gctgggtgga gcacgacccg caggcggtgt ggtgggcaga
      121 ctttgcggcg attagcaagg agctgacggc ggcggcgccc gaaccggtcg ccgcggtcgg
      181 catcagtggc atcggtccgt gcctgctccc ggtggacagc gaggaccggc cactgcggcc
      241 ggcgatcctg tacggcgtcg acacccgcgc caccgtcgag atcgatgagc tgacccaggc
      301 gctgggcaag caaaccatcc tggaacgctg cgggtcggtg ctcacctcgc aagcagtggg
      361 acccaagctg gcgtggctgc gcgcgaacga accggaaaac tggaccgcaa cccgccgctt
      421 cctgatggct agcagcttca tcgtgcgcaa gctcaccggc agctacatcc tcgatcacca
      481 ctcggccagc caatgcaccc cgatgtacga cccggtcgag caccgctgga tcgacgaatg
      541 gaccgagctg atcgcccccg gcctggagct gccgcagctg cactggccct ccgatgtggt
      601 tggcgaggtg cgggctgacg ccgccgagct gaccggcatc ccggcgggca cgccggtggt
      661 ggccggcacg atcgacgcct gggccgaagc caccagcgtc ggcgtcaccg cacccggcga
      721 cgttatggtg atgtacggga cgaccatgtt cctggtcgag gtgctggacc gtccgcgccg
      781 cagcccggat ctgtggggca ccgtgggcac ctttcccggc acccacaact tcgcggccgg
      841 aatggccacc tcgggcgcga tcaccgagtg gatccgcaag ctgaccgggg aaacgtcgtt
      901 cgacccgctc gtcgcggaag cccagcagtc gccccccggc gcacagggcc tgctcatgtt
      961 gccctacttc gccggggagc ggacaccgct gttcgacccg gatgctcggg gcgtgttgat
     1021 cgggctgacc ctgcggcaca accgcgggga tatctaccgg gcggccttgg aggccacggc
     1081 ctacggggtg cggcacaacc tgcaagcgat gcgcgacgcc ggtgggcaag cccgtcgcct
     1141 ggtcgcggtg ggcggcggca ccaagggagg gctgtggacg cagatcgtct ccgacgtcct
     1201 ggacgccccc cagcagctgc cccgccagac gatcggcgcg tgcctgggcg acgcgctgct
     1261 ggccgccatc gggatcggcc acatcaagag tggtgagtca cccgacacgc aaatagatat
     1321 ggccgaaata ggtatggcgc agtggaaccc catcgcggcc acgatcgagc cgcgcccgga
     1381 accggctgca acctacgcgc gtctttatga cctctatcgc gagctgtacc cgaacaccag
     1441 agagatcgcc cacgcgctcg cccgcgagca gcgcgtgctg tccccacgca tcggaggtag
     1501 ctgatggtcg cgtcctcgtc ccggtcccga gtgaccaccc ggcgggtgta tgccctgggc
     1561 gcgttggcgg cgttgttgtt tggttatgac aacggcatca tcggcgccgc gctgctgttc
     1621 atcccgcagg agttgccttt gagcccgcta caaaccggtg ctgtggtcag cgccacggtg
     1681 ttgggggcga tggtgggcgc actgggcagt ggtccggcgg ccgatcggct gggacggcag
     1741 cgggtcctgt tggttgccgg ggtggtgttc accgttggag cgctgggtac cggcctggct
     1801 gcgaccgtca atgggctggt ggggtttcgt ttcatcctgg gattggcgat tggtatcgcg
     1861 tcggtgaccg tgcctctcta cctggccgag atggcacctg cctccgaccg tggcacggtc
     1921 acctccctga accagtactt gatcattgtg gggacggccc tgtccgcggc tattggctat
     1981 gcactggctt tcactggttc gtggcggtgg atgttgctca ttggtgtgtt tcccgcgatc
     2041 gtgctgatgg ttgggatgtt caccatgccc gacacccccc gatcgctggt acggcgcgga
     2101 catcccgaac aggcgcgtga tgtcctggtc ggcttgcgtg ccgaccctga cctcgccgag
     2161 cgcgagctca ccgagatcac ggcactcgaa cagcagcagc gcgacacgcg caccggcgcc
     2221 cggctgtctg ccccctgggt gcgccggctg ctgctgctgg gcgccctgct ggcgattttc
     2281 cagcagatca ccgggatcaa cgcgatcgtg tactacgcac cgaccatcct caccggcttc
     2341 ggggtgtctg agaccaccgc attgctgttc ggtttcctca acggtttggt gaacatcgct
     2401 accatcgcgc tggttgtccg gtccaagatc attgatcggc gaggccgcaa acccgtcctg
     2461 ttagcggggc tggtgggcat ggcagccagc ttgttcgccg tcggtgcggc cgtgctgatc
     2521 ctcccggtca actcgactgc gctgtttgtc atcgcggcag cggcgttcgt ggtgttcacc
     2581 aacgccttct cggcgacttg gggcccggtg ttgtgggtga tgctggccga gatcttcccg
     2641 ctcgccatcc gtggcgtggc gatggccatc gcgaccttgt tcaactggct agccgccttc
     2701 gtcatttcac tgacgttccc gagcttcgtc gcattcgcgg gcatagggag cgccttcatc
     2761 gtctttgccg tgatcggcgt tgtgctcttc ccggtcgtgt ggtggatggt gcccgaaacc
     2821 aaggaacgca gcttggaatc gctggaaacg gcgtttcgta acggcagccg gatcctgccg
     2881 gctgagcgcg ccgctgccga gaccggaggg atggcatgac cgtcaactac ggcacctacg
     2941 gttcaagcct aagagaccta ccgcgactac gcagcacccg ccgacgccgg gcgtccagct
     3001 gggaccgcac cggtggtaac gacgaccgca tcaccctcca gcctgggcag accgccacgc
     3061 ttgcggacat cgatgcagcc ggcaccatca accacatctg ggtgaccgtc gccaacgaac
     3121 ggatgctgcg cgacccgtgg atgcgccagc ccgactacct gcgaaggctc gtgcttcgcg
     3181 catactggga cggcgaagag caccccagca tcgccgtacc cctcggtgat ttcttcggag
     3241 tcgggcacgg ccgcaccacc aatttcgtct ccgccccgct gcagatgagc ccagaagacg
     3301 gcaaagggtt caactgcttt tttcacatgc cgttcggcgc cggcgcccga ctggagattg
     3361 ccagcgaact gaccgatgag ctggtgtact tctattacta catcgattat gaggagctcg
     3421 acgagctcg
//
LOCUS       DATADN010000935         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_717859, whole genome shotgun sequence.
ACCESSION   DATADN010000935 DATADN010000000
VERSION     DATADN010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717859"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1655
                     /locus_tag="VHH53_12150"
     CDS             <1..>1655
                     /locus_tag="VHH53_12150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2290917.1"
                     /translation="GKCDPAELELTRRRPSPDAAGPMTVPAVTMAGVYAIDRFVEMLP
                     ALAASRRAEQAARVRTDADLLPLMRNALERTSPEIPYLLAHDAIVEALGVARVYGARR
                     RILVITADAVSERMAGPAIRAWNMAEVLSGEHDVRLISLNQHCSPPPAEFDVRQRTAK
                     SIGPELDWAQLVVLQGHVLEQIPSLKTSNHIVIVDIYDPMHLEQLEQGRDLGDGQRAQ
                     VVDAVTSVLSAQLRRGDFFLCASERQRHFWLGHLAAIGRLSPTVYDADPTTRSLLAVA
                     PFGLAGKPPQRTGPGLRDAWGFGSREKVVLWAGGVYSWFDPLTLLHAVHALRAEHDDL
                     RLVFLGMRHPNPDVPEMGMAGQTRRLAQTLGLTGEHVFFNETWVAYVDRQNWLLDADA
                     GVTTHFEHIETTFAFRTRVLDYLWAGLPIVTTDGDAFAELVAAEELGIVVPAENPVAL
                     AAALQRALYDDAFAAGCRDRIAGVAQRFTWEAVLAPLTGFCQHPRPAPDRLRGAALTA
                     AGCRNGMAGALRRDAALIRFYLAAGGPGEVARRAAQRLRRYAAR"
BASE COUNT          242 a          582 c          566 g          265 t
ORIGIN      
        1 ggcaagtgtg acccggctga gctggaactg acacgccgcc gcccgagtcc agacgcggct
       61 ggccccatga ctgtgcctgc ggtgaccatg gccggggttt acgccatcga tcggttcgtc
      121 gagatgctgc cagcgctggc cgcgtcgcga cgcgccgaac aggccgctcg ggtccgcacc
      181 gacgccgacc tgctgccgct gatgcgcaac gccctggagc gcacttctcc cgaaatcccc
      241 tacctgctcg cgcacgacgc gatcgtcgag gcactcggcg tggcacgggt atacggagct
      301 cgccggcgga tcctggtcat caccgcagac gcggtgtcgg agcggatggc agggccggcg
      361 atccgggcgt ggaacatggc cgaggtgctg tccggcgagc acgacgtgcg gctgatcagc
      421 ctcaaccagc actgctcgcc cccgccagcc gagttcgacg tgcggcaaag gaccgccaaa
      481 tcgatcggtc ccgagctgga ctgggcgcag ttggtggtcc ttcaaggcca cgtgctcgag
      541 caaatcccgt ccttaaagac gtccaaccac atcgtcatcg tcgacatcta cgacccgatg
      601 cacctcgagc aactcgagca gggccgtgat ctgggagacg gccagcgcgc ccaggtggtc
      661 gacgcggtca cctctgtgct atcggcccaa ctccgccgcg gcgacttctt cctgtgcgcg
      721 tcagaacggc agcggcattt ctggctcggg cacctggcgg cgatcgggcg gctgtccccc
      781 accgtctacg acgccgatcc gacgacacgc tcgctgctcg cggtggcccc gttcgggctg
      841 gccggcaaac caccgcagcg caccggaccg ggcttgcgtg acgcgtgggg gttcgggtcc
      901 agggagaagg tcgtgctgtg ggccgggggt gtctactcct ggttcgaccc gttgaccttg
      961 ctgcatgccg tgcacgcgct gcgcgctgag catgacgacc ttcgcctggt gttcctcggg
     1021 atgcgccacc cgaaccctga cgtgcccgag atgggcatgg ccgggcagac ccgccggctg
     1081 gcccagaccc tcgggctcac cggcgaacac gtgttcttca acgagacctg ggtggcctac
     1141 gtcgaccggc agaactggct gctcgacgcc gacgccggcg tcaccacgca cttcgagcac
     1201 atcgagacca ccttcgcgtt ccgcacccga gtgctggact acctgtgggc ggggttgccg
     1261 atcgtcacca ctgacggtga cgccttcgcc gagctggtag ctgccgagga gctcggcata
     1321 gtggtacccg ctgagaatcc ggttgcactg gccgcggcgc tgcagcgtgc gctctacgac
     1381 gacgccttcg ccgctggatg ccgggaccgg atcgcggggg tggcccagcg cttcacctgg
     1441 gaggcggtgc tggcgccgct gactgggttt tgccagcacc cacgaccggc tcctgatcgg
     1501 ctgcgtggcg cagcgctgac cgcggccgga tgccgcaacg ggatggccgg cgcgttgcgc
     1561 cgcgacgccg ctctgatcag gttttacctc gcggctggcg ggcccggcga ggtcgcccgg
     1621 cgtgccgccc agcggctacg ccgctacgcg gcacg
//
LOCUS       DATADN010000936         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_717953, whole genome shotgun sequence.
ACCESSION   DATADN010000936 DATADN010000000
VERSION     DATADN010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717953"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..445)
                     /locus_tag="VHH53_12155"
                     /pseudo
     CDS             complement(185..445)
                     /locus_tag="VHH53_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            471..2250
                     /gene="aceE"
                     /locus_tag="VHH53_12160"
                     /pseudo
     CDS             471..2250
                     /gene="aceE"
                     /locus_tag="VHH53_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
BASE COUNT          391 a          786 c          727 g          346 t
ORIGIN      
        1 cgcgtgcctt cctggagggc cgcctcaccg aggagaacct cgaccacttc cgccgcgagg
       61 cgctgacgcc ggagctcggc ctgtcgagct acccacaccc caggctcatg cccgacttct
      121 ggcagttccc gacggtgagc atggggtgtg ggtagctcga caggccgagc tccggcgtca
      181 gcgcctcgcg gcggaagtgg tcgaggttct cctcggtgag ccggccctcc agaaacgctc
      241 gcgcgtagat gccgggcgcc gcgtggccct ggaagaagat ctggtcgccg gggccctcgt
      301 ccgagccgcg gaagaagtgg ttgaacccga cctcgtacag gctcgcagca ctcgcgtacg
      361 tcgagaggtg cccgccgatc ccctcgaatc gcgtgttcgc ccgcagcacc atcgcgagcg
      421 cgttccatcg gatcatccgc tggaacgcgg tcgcgatggt cctccgcggc aacaaccagt
      481 acgcgggcct gggcggacac ctctcgacgt acgccagcag cgcgtccctg tacgaggtcg
      541 ggttcaacca cttcttccgt ggcaaggacc gcctgggcga gccgggcggc agcggcgacc
      601 agatcttcta ccagggccac gcggcgcccg gcatctacgc ccgcgcgttc ctggagggac
      661 gcctgtcgac gcagcagctc gacgggttcc gccaggagct gtcgcacggc ggtccgggca
      721 gcgggctgcc gtcgtacccg cacccgcggc tgatgccgga cttctgggag ttcccgacgg
      781 tgtcgatggg cctcggcccg ctgaacgcca tctaccaggc gcggttcaac cgctacctgc
      841 atgaccgcgg catcaaggac tccgacggcc agcacgtgtg gtgcttcctc ggcgacggtg
      901 agatggacga gccggagtcc cgcgggcaga ttcaggtcgc cgctaccgag gggctggaca
      961 acctcacctt cgtggtgaac tgcaacctcc agcgcctgga cggcccggtc cggggcaacg
     1021 gcaagatcat ccaggagctg gagtcgttct tccggggcgc cggctggaat gtgatcaagg
     1081 tgatctgggg ccgcgagtgg gatgccctgc tcttcgccga ccggcatggc gcgctggtga
     1141 acctgatgaa cgtcacgccc gatggtgact accagaccta caaagccaac gacggcggct
     1201 acgtccggca tcacttcttc ggtcgtgacc cgcgtaccaa ggagctggtc aaagacctgt
     1261 ccgacgccga catctggaac ctcaagcgcg gtgggcacga ctaccgcaag gtctacgccg
     1321 cctaccaggc cgccacccag caccacggcc agccgacggt gatcctggcc aagaccatca
     1381 agggctacgg cctgggcccg cacttcgagg gccgcaacgc cacccaccag atgaagaaga
     1441 tctccctgga ggacctcaag ctgctccgtg acgcccagcg catcccgatt accgatgcac
     1501 agcttgagga agaccccaca ctgccgccgt actaccaccc gggaccggac gcaccggaga
     1561 tccagtacat gtgcgagcgg cgccgcgcac tgggcgggtt cgtgcccgag cgacgcacca
     1621 agttcaagcc cctcgtgctg cccggtgaca aggcctatga cgtgctgcgg cgcgggtcag
     1681 gcaagcagga ggtcgccacc acgatggcgt tcgtccgact ggtccgcgac ctggccaagg
     1741 atcccgagat cgggccgcgg ctggtgccga tcatcccgga tgaggcacgc accttcggca
     1801 tggattcgat gttccccacg cagaagatct acaacccgca cggacagctc tacacctcgg
     1861 tggacgccga gttgatgttg gcttaccggg aaagcgagca gggccagatc ctgcacgagg
     1921 ggatcaacga agcgggctcc accgcgtcgt tcaccgcagc gggcacctcc tacgccacgc
     1981 acaacgagcc gctgattccg gtctacatct tctactcgat gttcggcttc cagcgcaccg
     2041 gtgacggcct gtgggcagcc ggcgaccaga tggcccgtgg gtttctgctc ggcgcgaccg
     2101 ccggacgcac cacgctggtc ggtgagggcc tgcagcacaa cgacgggcac tccctgctgc
     2161 tcgcctcgac gaaccccgcg gtcatctcct acgacccggc gtacggctac gagatcggtc
     2221 atatcgtgcg gtcggggctc gaccgcatgt
//
LOCUS       DATADN010000937         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_425204, whole genome shotgun sequence.
ACCESSION   DATADN010000937 DATADN010000000
VERSION     DATADN010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425204"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1619)
                     /locus_tag="VHH53_12165"
     CDS             complement(<1..>1619)
                     /locus_tag="VHH53_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX2290918.1"
                     /translation="GAEDITGIFINTLPVRVEIDHTLGVMPWLQQIQATQAEARQFDF
                     VSLAHLQTLSDIPAGTNLFNSLVVFENYPINDEVAATHGLQLREVHASETTNYPLTLI
                     AIPNHQLSLQLGYDPDLFDPATIERMASHLQMLLVGIVEDANRLVGDLPMLTEAETRQ
                     VLVEWNDTDQPMLPAVLPEVLQAQVARTPEATAVVCEGIELSYAQLNARANRLARLLI
                     DYGAGPERVVALALPRSVELIVALVAVLKTGGGYLPIDLAYPPDRIAFMLADADPVLV
                     VTTTELADQLPASEVVRVVLDEPDTVARVGSYRHVNVDDDERGGVLSASNAAYVIYTS
                     GSTGRPKGVVVSLGGLGNFLAAMQQRFVLGAGDRLLAVTTVGFDIANLEVFLPLLHGA
                     AVVVAGREVAADPLWLGHTVASAGVTVMQATPSLWRAVVTEGAAELAGVRVLVGGEAL
                     PADLAGALVDCAVSVTNLYGPTETTIWSTVAGVDQHTARNPSIGRPIANTQVYVLDAG
                     LRPVPVGAVGELYIAGAGLARGYLHRAGLTA"
BASE COUNT          319 a          543 c          495 g          262 t
ORIGIN      
        1 ccgcggtcag ccccgcacgg tgcagatacc cccgggccaa cccggcgccg gcgatataca
       61 actcgcccac agcgcccacc ggaaccgggc gtaacccggc gtcgagcaca tacacctgcg
      121 tgttagcgat cggtcgacca atcgaggggt tccgcgcggt gtgttggtca accccggcca
      181 ccgtggacca aatcgtcgtt tcggtgggcc catacaggtt ggtcaccgac accgcgcagt
      241 ccaccaacgc gccagccaag tcggcgggca aggcctcacc gccgaccaat acccgcacac
      301 cagcaagctc agcggcgccc tcggtgacca ccgcacgcca caagctcggc gttgcctgca
      361 tcaccgtcac accagccgat gccaccgtgt ggcccagcca caacgggtca gcggccacct
      421 cacgaccggc caccaccacc gccgccccat gcagcagcgg aaggaaaacc tcaaggttgg
      481 cgatgtcaaa cccgacggtg gtcaccgcca acagccgatc acccgcaccc aaaacgaacc
      541 gttgctgcat cgcggccagg aaattgccca acccacccaa ggaaacgacc acacccttgg
      601 ggcggccggt ggaccccgag gtgtaaatca catacgccgc attcgatgcg gacaacacgc
      661 caccacgctc gtcgtcgtcg acgttgacgt gccgatagct gcccactcgc gccactgtgt
      721 cgggctcatc caacaccacc cgcacgacct cactagccgg cagctgatcg gccaactccg
      781 tcgtcgtgac caccaacacc ggatcagcat cagcgagcat gaaggcgatc cggtcgggcg
      841 gataagccag atcaatcggc agatatccac caccggtctt cagcaccgcc accaacgcca
      901 cgatcaactc caccgaccgc ggcaacgcca acgccaccac ccgctcaggc ccggccccat
      961 agtcgatgag cagccgcgcc agtcgattcg cccgcgcgtt gagttgcgca taggacaact
     1021 cgatgccctc acacaccacc gctgtcgcct caggagttcg ggctacctgg gcctgtaaca
     1081 cctcgggtag cactgccggc agcatcggct ggtcggtgtc attccattcc accaacacct
     1141 gccgggtctc ggcctcagtg agcatcggca ggtcgcccac cagccggttg gcgtcttcaa
     1201 caatcccgac cagcagcatt tgcaggtggc tggccatccg ctcgatggtg gccgggtcga
     1261 acaggtcagg gtcgtagccg agttggaggg atagttggtg gttggggatg gcgatgaggg
     1321 tgagggggta gttggtggtt tcactggcgt ggacctcgcg tagttggagg ccgtgggtgg
     1381 cggcgacttc gtcgttgatg gggtagttct cgaagacgac gaggctgttg aataggttgg
     1441 tgccggcggg tatgtcgctg agggtttgta ggtgggccag ggacacgaag tcgaattggc
     1501 gggcttcggc ttgggtggct tgtatttgtt gtagccaggg catgacaccg agggtgtggt
     1561 cgatttcgac gcggacgggc agggtgttga tgaagatgcc ggtgatgtcc tcagcaccg
//
LOCUS       DATADN010000938         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_256663, whole genome shotgun sequence.
ACCESSION   DATADN010000938 DATADN010000000
VERSION     DATADN010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256663"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..462
                     /locus_tag="VHH53_12170"
     CDS             136..462
                     /locus_tag="VHH53_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX2290919.1"
                     /translation="MTCPKCHGSLRTVDKQGVRLGQCENCRGIFLDHGELEQLLRAEQ
                     HYYGGGVAPYVGHSGGHRGHSDSPRGYRDSPRPYREGHHGHYADSPRPYGHRRSHGSF
                     LGGLFG"
     gene            481..1083
                     /locus_tag="VHH53_12175"
     CDS             481..1083
                     /locus_tag="VHH53_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2290920.1"
                     /translation="MNPRICDRCGCLLELPLVCGECGHRQSLARQPLFCLAGPSGTGK
                     STVARALLPRLSDRFVILEQDVLWLPELSDPTDEHRLFRSTWLRLAAMIGCSGRPVLL
                     CGTVVPPELEPLPERALFSAVHYLALTCRPDVLAQRLRARPAWREWDEPRIAETLDYT
                     SWLGACAATLNPPVTLLDTTGSSVDATAHDVCSWLAQLTS"
     gene            1228..1578
                     /locus_tag="VHH53_12180"
     CDS             1228..1578
                     /locus_tag="VHH53_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290921.1"
                     /translation="MADNEPTTDVACSDPELSEELRDALVLLRDRSDNDSFRALVDDL
                     LVGRCSLLEASGTAAFSDVVFARIAQEFNELTEDEKRNLVGQSELSSSAGSCGIPCAG
                     CPGLCATPRDSRSS"
     gene            complement(1727..1906)
                     /locus_tag="VHH53_12185"
     CDS             complement(1727..1906)
                     /locus_tag="VHH53_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290922.1"
                     /translation="MCGLIGALLAESDLTRVRIAATLASIGHRGPDHACWWRSSDRRM
                     MLGRIPTTPAASPSP"
     gene            2072..2263
                     /locus_tag="VHH53_12190"
     CDS             2072..2263
                     /locus_tag="VHH53_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290923.1"
                     /translation="MSDDALEEFRQASTGWALEQELEVAAEVYGPGPTGLRTPPSELS
                     DEELLERYKSMLGFDLKWE"
     gene            complement(2457..>2762)
                     /locus_tag="VHH53_12195"
     CDS             complement(2457..>2762)
                     /locus_tag="VHH53_12195"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEX2290924.1"
                     /translation="GTPSSSPIQLTGREKQMPQFLDHHPIAHALLTEEAVEEIRAQIR
                     AEIPNDFGVKWLNAFGAANGEGYCLSEAPNAEAVVKSHEALGYLIETTDVIEVTSLT"
BASE COUNT          454 a          918 c          888 g          502 t
ORIGIN      
        1 gcccgctgtc gagttgtcca gccctgggtt ttggtgccat cccgcccgcc tccccgtcct
       61 tagggaaggc tacctatatt caggcggcgc tgacgcgctg tgctagccga gcacgacgga
      121 tcgtatgctg cttgagtgac atgtccgaaa tgccatgggt ctctgcgaac cgtcgacaag
      181 caaggggtgc gcctggggca gtgcgagaac tgccggggca tcttcctcga ccatggcgag
      241 ctggaacagc tccttcgggc cgagcagcac tactacggcg gcggggtggc cccgtatgtg
      301 gggcattcgg gcgggcaccg cggccactca gactcgccta ggggttaccg ggactcgccc
      361 cggccctacc gggaaggtca ccacgggcac tacgccgact cgccgcgccc ctacggacac
      421 cggcgcagcc acggaagctt tctcggcggc ctgttcggct aggtcacgcg ccggtaccgg
      481 atgaacccgc ggatctgtga ccgctgtgga tgcttgctag aactgccgct ggtctgtggc
      541 gagtgcggcc accggcagtc cttggcccgc caacccctgt tctgcttggc aggccccagc
      601 ggcaccggca agtccacggt ggcccgcgcc ctgctgcccc ggctgtctga ccgcttcgtg
      661 attctcgagc aggacgtgtt gtggcttcct gagctcagcg atcccaccga tgagcaccgg
      721 ctgttccgct cgacgtggct gcggctggcc gcgatgatcg gatgcagcgg acggccggtg
      781 ctgctgtgcg ggaccgtagt gcctcccgag ctggagccgc tgcccgaacg cgcattgttc
      841 agcgccgtgc attacctggc gttgacctgc cgacccgacg tgctggcgca gcggctgcgc
      901 gcgcggcccg cgtggcggga gtgggacgag cccagaatcg ccgaaacgct cgactacacc
      961 tcgtggctcg gcgcctgcgc ggcgacgctg aacccgccgg tgaccctgct ggacaccacc
     1021 ggcagcagcg tggacgccac tgcccacgac gtctgcagct ggctcgcgca gctcacctcg
     1081 tagaccggcg ctacccgatc gatgcgtgcg tgaacggcgc ggtcggtgtg tggatcggag
     1141 ccgcgacgtg cacaataaaa cgtgtggcgt gttccacgag ctgccggacg cgccgcctgc
     1201 gacgccaacc accaccgatg aggagcgatg gctgacaacg agcccacgac cgatgtcgcg
     1261 tgcagtgacc cggagctgtc cgaggagctt cgtgacgcgc tggtcctact gcgtgaccgc
     1321 agcgacaacg acagcttccg cgccctggtc gacgatctgc tggtcggacg gtgcagcctt
     1381 ctggaggcgt cgggcacagc agcgttcagc gatgtcgtct tcgcacggat cgcccaggag
     1441 ttcaatgagc tgaccgagga cgagaagcgc aacctcgtcg ggcaatccga gctatccagc
     1501 tcagccggct cctgcggtat cccgtgcgcc ggctgccccg gcctctgcgc gactcccagg
     1561 gacagccgat ccagctgacc gcccgggcga ggcgatcgtc gaaccatcga cgcaccaccg
     1621 ccacgagcga gcacctgttt gctcagccac gcgttgggat ctgattctgc cggctgcagg
     1681 tgcgatacgg cagcgggccc actacctaga tgccgtgtcc catagatcac ggcgagggac
     1741 tcgcggctgg ggtagtcggt atccggccga gcatcatccg gcgatcagac gagcgccacc
     1801 agcaggcgtg gtccggtccg cggtgcccga tcgacgcgag cgtggccgcg atgcgcaccc
     1861 gcgtcaggtc ggactcagcg agcagcgccc cgatcaaacc gcacatcggc ttgctccctc
     1921 cggatcaggt gtcatcgtga tctccgtcca cttgccggcg gcaccacgag gatgaagagg
     1981 cggcagcgac ctgtctgaat acgacagcga ggcgctcgtc ctccccggcg tagctgggta
     2041 ccgtcggtgt gtccgcttcg gaggaggtgc catgagcgac gacgcactcg aggagtttcg
     2101 ccaggcctcg acgggatggg cgctcgagca ggagctggag gtggcagcag aggtctacgg
     2161 tcccgggccc accggacttc gcacgccacc gtcggagttg agtgacgagg agcttcttga
     2221 gcgatacaag tctatgctgg ggttcgatct gaaatgggag tagccggtcg gctgtcgccc
     2281 acccggaccg atcagcggct tcgactgcag ccgtgatcgt tcagcttatt cttactcgct
     2341 cagctcaacc gtcgggcgtc gaatacagtg acgtaggcac ttcagtagtg cggtcctgcc
     2401 cgcgccgttt gaaccgatga cactgcaggt gcctcggagt cctgccatcg tgctgtctat
     2461 gtcaggctag tgacctcaat gacgtccgtc gtctcaatca ggtatcccaa tgcttcatgt
     2521 gacttgacca ccgcttctgc gtttggcgcc tctgaaaggc aatacccctc cccgttggcc
     2581 gctccaaatg cgttcaacca tttcacgccg aagtcattcg gtatttcggc tctgatctgg
     2641 gccctaatct cttctacggc ctcctccgtc aataaggcat gtgcgatggg atgatgatcc
     2701 aaaaactgcg gcatctgctt ctcccttcca gtaagctgta tcgggctact actgggcgta
     2761 cc
//
LOCUS       DATADN010000939         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_100110, whole genome shotgun sequence.
ACCESSION   DATADN010000939 DATADN010000000
VERSION     DATADN010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100110"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(570..>1865)
                     /locus_tag="VHH53_12200"
     CDS             complement(570..>1865)
                     /locus_tag="VHH53_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2290925.1"
                     /translation="KNMIKAFFFDLQHINKGGSRPDGYPQRTFNKVGVLGAGMMGAGI
                     AYSCARAGMDVVLKDTDLAAASRGKSYSARLLDKAVSQGRSTPDQREEMLTRIIPTAR
                     VEDLKGCDLVIEAVFEDPAVKKQVFAEILDVVDPDALLCSNTSTLPITDLAGGVDRPA
                     DFLGLHFFSPVDKMPLVEIVRGQQTSDAALAAAIDVVQQLRKTPIVVNDSRGFFTSRV
                     IGTFINEGVAMLAEGAAPASIEQASAQAGYPAPVLQLVDELTLTLMRKIREETRRGIE
                     AAGGTWTPHPADAIVDRMVLEFERPGRSGGAGFYDYTDGRRTRLWPGLAEHFGPSRPD
                     AIPFADMKERMLFAEALETAKCFDEGVITSVPDANIGSIMGIGFPPWTGGVVQFMIGY
                     PGGLPGFVARAKDLADRYGDRFAPPPSLIAKANAEDSFT"
BASE COUNT          325 a          617 c          613 g          310 t
ORIGIN      
        1 gcggccgtcg accgtgattt ggctatatgc gcgggttggg gcgacagatg tgctacgcca
       61 ccaggtacgg cgtcgccgtc tagtggccac cgacaccgca ctgagttcga tcccggatga
      121 aagggggccg agcccacaaa acggaaggag tgagaaccca tcctctggca agaaatcttc
      181 tttttctcac ctagcagcgt taccgctggc ggtgaaggag ccacccacgg gatggacaac
      241 cgcaacggat cgacatccgg ctgacgcgac cgttagccgc ggcaaaggag taaaaaacga
      301 acgaccacac catgcgctgc gtggaccggc cgcgctgcat atgccgctgc aggccgtgcc
      361 catcagccgc aacgtgccga cggctgcact cgccggaacc ccgggagtcg cggcgaactg
      421 ggcattccta cctgcactcg ccgcgtgggc ggcgccgaag ttagagcttt aacctgagcc
      481 ctgggactgc acgggcggcg gttgtgcaca ctgccggccg cccgtgcggc gtccccctcc
      541 gcccctagcg actgagcgcg gtcgccctgt catgtgaagg aatcttccgc gttggctttg
      601 gcgatcaggg acggcggtgg ggcgaagcgg tcaccgtagc ggtcggccag atccttggcg
      661 cgggcgacga agccgggtag gccaccggga tagccaatca tgaactgcac caccccgccg
      721 gtccacggcg ggaacccgat acccatgatg gaaccgatgt tggcatcggg aaccgaggtg
      781 atgacgccct cgtcaaagca tttcgcggtc tccagcgcct cggcgaacag catccgttcc
      841 ttcatgtcgg caaacgggat cgcatctgga cgagacgggc cgaagtgctc ggcgaggccg
      901 ggccacagcc gggtgcgccg gccgtcggtg tagtcgtaga acccggcacc gcccgagcgc
      961 cccgggcgtt cgaactccag caccatccgg tccacgatcg cgtcggccgg atgcggtgtc
     1021 caggtgccgc cggccgcctc gataccacgc ctggtctcct cccggatctt gcgcatcagc
     1081 gtcaacgtca gctcgtcaac cagctgcaac accggcgccg ggtagccggc ctgggcactg
     1141 gcctgctcga tggaggccgg ggccgcgccc tcggcgagca tggccacacc ctcgttgatg
     1201 aaggtgccga tcacccggct ggtgaaaaag ccccggctat cgttgaccac gatcggggtc
     1261 ttgcggagct gctgcacgac gtcgatggcg gcggccagcg ccgcgtccga cgtctgctgc
     1321 ccgcggacga tctccaccag cggcatcttg tccaccggcg agaagaagtg caggccgagg
     1381 aagtctgccg ggcggtcaac cccgccggcg aggtcggtga tcggcagcgt cgaggtgttc
     1441 gagcacagca gggcgtcagg atcgaccacg tcgaggattt cagcgaagac ctgcttcttc
     1501 accgctggat cttcgaaaac cgcctcgatt accagatcgc agcccttgag atcctccacg
     1561 cgggcggtcg ggatgatccg ggtcagcatt tcctcgcgct ggtctggggt ggatctaccc
     1621 tgggacacgg ccttgtccag cagcctggcc gaatagctct tgccccggct cgccgctgcc
     1681 aggtcggtgt ccttgagtac gacgtccatg cccgcgcgag cgcaggagta ggcgatgcct
     1741 gcgcccatca ttcccgcgcc gagcacaccg accttgttga aggtgcgctg ggggtatccg
     1801 tcgggccggc tgccgccctt gttgatgtgc tggaggtcga agaagaacgc cttgatcatg
     1861 ttctt
//
LOCUS       DATADN010000940         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_718466, whole genome shotgun sequence.
ACCESSION   DATADN010000940 DATADN010000000
VERSION     DATADN010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718466"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VHH53_12205"
     CDS             complement(<1..591)
                     /locus_tag="VHH53_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2290926.1"
                     /translation="MITGADSGLGRNVAPGLAAIEIKHRVVEVHGLRMRLAEAGSGPL
                     VVLLHGFPECWYSWRHQLRALADAGFHAVAPNQRGYPGTDCPAAVADYTMLHLVGDVV
                     GLIGALGEHNAVVIGHDWGALVAWHTALLRADLVRGVGGLSVPFSARTPVPTLTAVRS
                     RFGDDVYLLYFQHPGVEKDFESDLTESFRRLLAGVSG"
     gene            complement(638..1348)
                     /locus_tag="VHH53_12210"
     CDS             complement(638..1348)
                     /locus_tag="VHH53_12210"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX2290927.1"
                     /translation="MARVFVSHASEDRECAGQLHQWLVREGHEVFLDQDLRDGIPVGG
                     EWDKLLHERLRWADAVVCVVTSASVVSPWCAAEVGIAVSRGSRVLPVRAEPGVDHPLL
                     RSAQYADLNQDPTRARAALVEELRRVDAVGGLGWPDDRSPFPGLRPFDVEDHRVFFGR
                     SGEVEQLAGLLRSPAERAEKATTNQPVPSQSHGQTPTRHVSAHVRDTTMRDRAGRINC
                     FSCSHRQIVKHVGRGVPV"
BASE COUNT          315 a          579 c          455 g          247 t
ORIGIN      
        1 gccagagacg ccggctagca accgccgaaa cgactcagtc aggtcggact cgaagtcttt
       61 ctccacccct gggtgttgga agtacagcag gtagacgtcg tcaccgaagc ggctgcgcac
      121 tgcggtcagg gtgggcaccg gcgtgcgcgc ggagaacggc acgctcagcc cgccgacgcc
      181 gcgcaccaga tccgcgcgca gcagcgcggt atgccatgcc accagggccc cccagtcgtg
      241 gccgatcacc accgcgttgt gctcgccgag cgcaccaatc agccccacca cgtcgccgac
      301 caggtgcagc atcgtgtaat ccgcgacggc agccgggcag tcggtacccg gatagccacg
      361 ctggtttggc gcgacagcgt ggaagccggc atcggccagc gctcgcaact ggtgccgcca
      421 cgaataccaa cactccggga aaccgtgcaa cagcaccacc agcggaccgc tgccggcctc
      481 ggccaggcgc atccgcaatc cgtggacctc gacgacgcgg tgcttgatct cgatggccgc
      541 cagacccggt gcgacgtttc gtcccagccc ggagtccgcg ccggtgatca ctgtggttgg
      601 ttcgttgctc cctgacattc ctcggtcttc tctctggtta tacgggcact ccccggccta
      661 cgtgcttgac tatttgacga tgggaacatg aaaagcaatt aatccgtcca gctctgtccc
      721 tcattgtggt gtccctcacg tgtgcgctga cgtgtctggt cggtgtctgg ccatggcttt
      781 gcgaagggac tggttggttc gttgtcgctt tctcggcgcg ctcggccgga gaccgcaaca
      841 gcccagcgag ctgctccacc tcaccgctgc gaccgaaaaa cacccgatgg tcctcgacgt
      901 cgaacgggcg cagccccgga aacggcgacc gatcatccgg ccaacccaac ccccccacgg
      961 catcaacccg acgcaactcc tccaccaacg ccgcacgtgc cctagtggga tcctggttga
     1021 ggtcggcgta ttgcgcggat ctcagcagcg ggtgatccac ccccggctcg gcccgcaccg
     1081 gcaacacccg gctaccccgc gacactgcaa tgcccacctc ggctgcacac cacggtgaga
     1141 ccaccgatgc ggaggtgacc acacagacca ctgcatcggc ccaccgcaac cgctcatgca
     1201 acagtttgtc ccactccccg ccgaccggga tcccatcacg gagatcctga tccaggaaca
     1261 cctcatggcc ctcgcgaacc agccactggt gtagttgacc ggcacactca cggtcctcgc
     1321 tggcatggct gacgaacacc ctggccaccg cacctcccct gaccagggta atagcacccc
     1381 cgacagagtc agcagaaaat cagcaaagca accgtgacac ccggtacggg ccgagaccga
     1441 ccagcacgaa ccacgacgta gacgacacgt gacggtcgtc aacacgtttg gtcggccgtg
     1501 atccgcgacg tccacactga agtgacatgc caggtctggg ggcggccccg gcaaaaaatt
     1561 gggtacaaat cgcaccaccc ggcgtcagcc aacgtc
//
LOCUS       DATADN010000941         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1478488, whole genome shotgun sequence.
ACCESSION   DATADN010000941 DATADN010000000
VERSION     DATADN010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478488"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VHH53_12215"
     CDS             complement(<1..248)
                     /locus_tag="VHH53_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX2290928.1"
                     /translation="MSRQALEDDGPLDSLTRETLLYCWGFCAALSGHHRSEDQVLFPQ
                     LSQAHPDLVPVIRQLTQDHNMIGHLIGQLERSLDSVTTT"
     gene            complement(363..2084)
                     /locus_tag="VHH53_12220"
     CDS             complement(363..2084)
                     /locus_tag="VHH53_12220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2290929.1"
                     /translation="MTRLALCGGTYSNPYALRAFVADARARGADRLWCLGDFGAYGAE
                     PEAIWPLLTGNGIECIAGNYELAIGRGDPDCGCGYADDDDNAFAAIAYDYTLRHTSRD
                     FAAWMRTLATEHREMVGGVGLHLVHGSPLAVNDFWWESLPDDAHRMRIDASGADVICC
                     THSGLPWIRHFPDGAVGEQAASSGARRDTLVVNVGVLGRPANDGGQHVWYALLDCEDG
                     NATAELVALDYDWPAHAASMRNAGLPEVFTQAVETGWWTTCLEIVPPVERSRGRFQLY
                     KSAMPAFAGAEVSWAQTPEIPDNGLPVLPLFGTALFPPRLWVYTNFHCNLACNYCSVA
                     SSPQARRRQLGLPRFRELVDEAVAEGFTELYVTGGEPFLERDLVEMLLYATEQLDVVC
                     LTNAMLYQGWRRTQLERLAGRQRLILQTSLDGAQAQFHDAHRGRGSWAKAMAGLDLAL
                     SLGLPVRVGMTETPQNCAQVEPLRALLATKGIRGRDFAVRPLVKRGYSDEGVAISEDN
                     TVPELTVTADGWHWHPAGADLDTSPDMLLAGPDVSMAEAKRRAVELFLRLRQRDGSLP
                     LVYNCAV"
BASE COUNT          399 a          807 c          823 g          372 t
ORIGIN      
        1 gtcgtggtga cgctgtccag ggagcgttcg agctggccga tcaggtgccc gatcatgttg
       61 tggtcctgag tgagctgcct gatcacgggc acgaggtcgg ggtgggcctg gctcagctgc
      121 gggaagagga cctggtcctc gctgcggtga tgaccgctga gggcggcaca gaacccccag
      181 cagtacagca gcgtctcgcg ggtcagcgag tcgaggggcc catcgtcctc cagcgcctgc
      241 cgggacaacg aaagagctgc ccgtagacgc gcgcgtggac ttcgcgcacc tggcggctcc
      301 acgcccggac acgctccgcg ctcggccctc gcccagcgcg ccaggcaggc cgtgacggga
      361 catcagacag cgcagttgta gaccagcggt agtgagccgt cgcgctggcg aagccgcaga
      421 aacagctcca ccgctcgccg tttggcctcg gccatgctga cgtcgggtcc ggcgagcagc
      481 atgtccggcg aggtgtcgag gtcagcgccg gctgggtgcc agtgccaacc gtcggcagtg
      541 acggtcaact cgggcacggt gttgtcctcg ctgatcgcga ccccctcatc ggaatatccc
      601 cgcttgacca gcggccgcac cgcgaagtcc cggccccgta tgcccttggt ggccagcagc
      661 gctcgcagcg gctcgacctg ggcgcagttc tgcggcgtct cggtcatacc gacccgtacc
      721 ggcaggccca gtgacagcgc caggtcaagg ccggccatcg ctttggccca ggaccctcgc
      781 ccccggtgcg cgtcgtggaa ctgggcctgc gccccatcca gcgaggtctg caggatcagc
      841 cgctggcgac cggctaaccg ctccagctgg gtgcgtcgcc agccttggta cagcatggcg
      901 ttggtcaggc agaccacgtc gagttgctcg gtggcataga ggagcatctc gacgaggtcg
      961 cgttcgagaa agggctcgcc accggtgaca tacagctcag tgaagccctc cgcgaccgcc
     1021 tcatcgacca gctcgcgaaa ccgcggcagg ccgagctggc ggcggcgggc ctgcggggac
     1081 gaggccacag agcagtagtt gcaagccaga ttgcagtgga aattggtgta gacccacagg
     1141 cgcggaggga acagtgcggt gccgaacagc ggcagcaccg gcaggccgtt gtccgggatc
     1201 tccggcgttt gcgcccagga cacctcggcg ccggcaaaag ccggcatcgc cgacttgtag
     1261 agctgaaagc ggcctcggga gcgttccacc gggggcacga tctccaggca ggtggtccac
     1321 catccggtct ccacggcctg ggtgaatacc tcgggtaagc cggcgttgcg catcgacgcg
     1381 gcatgtgcgg gccagtcgta gtccagcgcc accagctccg cggtggcatt gccgtcctcg
     1441 cagtccagca acgcgtacca gacatgttga ccaccatcgt tggccggtcg gccgagcact
     1501 ccgacgttga ccaccagcgt gtcgcggcgg gcaccggagc tggctgcctg ttccccgacg
     1561 gcaccgtcgg ggaagtgtct gatccacggc agcccggaat gcgtgcagca gatcacgtcc
     1621 gcgccggacg cgtcgatccg catccggtgt gcgtcgtcgg gcagcgattc ccaccagaag
     1681 tcgttgacag ccagcgggga gccgtgcacg agatgcaggc ccacgccgcc gaccatctcc
     1741 cggtgctccg tggccaaggt gcgcatccag gccgcgaagt cccgggaggt gtgccgcagc
     1801 gtgtagtcgt aggcgatcgc ggcgaaggcg ttgtcgtcgt catcggcgta tccgcagccg
     1861 cagtcgggat ctccccggcc gatggcgagt tcatagttac cggcgatgca ctcgatgccg
     1921 ttgccggtca gcaacggcca gatcgcctcc ggttcggccc cgtaggcgcc gaagtcaccc
     1981 aggcaccaca gccggtcggc gccacgggct cgcgcgtcgg cgacgaaagc tcgcaacgcg
     2041 tacggattgg agtaggtccc accgcacagc gccaacctgg tcacagcgcc aacttcgtca
     2101 cagagccagc ctcatcatca acgctccttc cgttcgggac gctaccgtgc tgttcttacc
     2161 gggctggcga accggttcgg gccgatcagc gaaatcaagg gcgtctgccc gccgcgaacc
     2221 gcggccacgg aaaggcgtgt gcatgatgcg attgatcgac atgttgcggc ggctcgaacg
     2281 tcggacccgg ccggttgatc cggagttcgc cgaggtgctg caccgccgat gggcggagct
     2341 tccccagcat gtcaaaacgc cgggccagtt tctcggcagg cacgcggtgg gctgcgaagg
     2401 t
//
LOCUS       DATADN010000942         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1028684, whole genome shotgun sequence.
ACCESSION   DATADN010000942 DATADN010000000
VERSION     DATADN010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028684"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..460)
                     /locus_tag="VHH53_12225"
     CDS             complement(50..460)
                     /locus_tag="VHH53_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290930.1"
                     /translation="MTSTSRNFAAILLLLLVAVEFGGGSLLTLLTRRIPGYLDNPLRQ
                     NMWRAGHAHPGVWLVLGLVGMLYVDQTPLSDGLKSLVRVTLLLAPILVSLGFFLSVLS
                     PKAERPNGLINLIYLAALLLAVGVVTLGIGLFQA"
     gene            complement(762..1316)
                     /locus_tag="VHH53_12230"
     CDS             complement(762..1316)
                     /locus_tag="VHH53_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290931.1"
                     /translation="MHTEYFHRWMDLLVARLEPAEPVLARGRAFEPRDALGFDLATAI
                     SGPGCAVVVTERCVLWVSRDDQRWVRSLPLDLVRSYTEITQAHRYALALDHRSIDRLQ
                     WVPAHQLLWWSWGNAEAVRPVTSTVLAFSRRDTEAARAIRSRLRAIGVPVSEPRSLPK
                     HRRPDEVGELRRHASRPQCGPGAG"
BASE COUNT          327 a          642 c          598 g          269 t
ORIGIN      
        1 ggggaaccgg cctcgccctc cggtcaccgt ggagtttgtg cgctcaggat cacgcctgga
       61 acaggccgat gcccagggtc accactccga ccgccagcag gagcgcggcc aggtagatca
      121 ggttgataag cccattgggc cgttccgcct tcggcgacag caccgacagg aagaagccga
      181 gggagaccag gatcggcgcc aggagcagcg tcacccgtac cagcgacttc aggccgtccg
      241 acagcggcgt ctggtcgaca tagagcatgc cgaccaggcc gagcacgagc catacgcctg
      301 ggtgggcgtg cccggcccgc cacatgttct ggcgaagcgg gttgtcgagg tagccgggga
      361 tgcgccgggt cagcagggtg agcagcgaac cgccaccgaa ctccaccgcg accagtagaa
      421 gcagcaggat cgctgcgaag ttcctggaag tagaggtcat tggctctctc cttgcggcca
      481 ttggtgaccg cttgcgttat cgtccggcgc atcctaactg cgccagtact gcactcaacg
      541 atggactggg gccagatcgg agccacagcc gatcggagga tcgcggacaa cagcggacac
      601 catcggtcgg gtaacgccct gcctagcggc atccatcaac cagcattgct ggtggcctga
      661 ccccccggtt cgctctcgca cggaggaagc caggggttca aatcccctca cctccaccca
      721 gcctcgcagt gcaaagcgtc gccgaaccac atcggcggcg ctcaccccgc tccggggccg
      781 cactgaggcc gcgaggcatg gcggcgcaac tcgcccacct catccggccg tcggtgcttc
      841 ggcagcgagc gtggctcgct gaccgggact ccaatcgccc gcagccgact cctgatcgct
      901 cgtgccgcct cggtgtcgcg acggctaaat gccagcacgg tgctggtgac aggtcggacc
      961 gcctcggcgt tgccccagga ccaccagagc agctggtgcg ccgggaccca ctgcaagcgg
     1021 tcgatgctcc ggtggtccag ggccagcgca taccgatgcg cctgggtgat ctcggtgtag
     1081 gacctcacca gatccaacgg gagcgacctg acccatcgtt ggtcgtcgcg gctgacccac
     1141 agcacacagc gctcggtcac gaccaccgcg caaccaggac cgctgatcgc cgtggccagg
     1201 tcgaacccga gcgcgtcacg tggttcgaag gccctgcccc gcgccagcac cggctcggcc
     1261 ggttcgagcc gggcgacgag cagatccatc catcggtgga aatactcggt gtgcatcgcg
     1321 gctcagtgtc acacggatcc ccggcgtgct ggtgcaagca ccgtcgttct cgtcggccgt
     1381 gccacccgcg taccacatca gacggcctcc aggccgacga cgagaagccc cagaccatag
     1441 agccctttga ccctccccta tcgtctgcca gttctgcggc aagcccttcg agcaggtcgc
     1501 gggactcttc gaggcgaagc gtcgcgtcag ggatccaaac acctcagccc tggcggcagt
     1561 gtggatctgc aacgagtgcg ttgcccggat ggcgcagcta ctggccgagg agccccgagg
     1621 accccagtgg ctcgaagatg gcggcatgga cacctggcgg cctcatgcga ccgtaaggat
     1681 cggcctttcc gtgcccggcc ggccgaaccg gtccctggtc gcggaggatc ggagcgccgg
     1741 aggctccgcg accagacggg accagacgct cccctggtcc gcgctgggtc cgcaccaggt
     1801 cgggaacccc cggttctcag cgggcacgag cggagg
//
LOCUS       DATADN010000943         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1310134, whole genome shotgun sequence.
ACCESSION   DATADN010000943 DATADN010000000
VERSION     DATADN010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310134"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1697
                     /locus_tag="VHH53_12235"
     CDS             456..1697
                     /locus_tag="VHH53_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2290932.1"
                     /translation="METLVERCAGLDVHKDSVTACVRVPDGQGRRQAETRRFTTTTAG
                     LGLLAQWLASFGVTRVGMESTGCYWKPVWQLLEGQMECWLRNAAHLHNVPGRKTDVAD
                     AAWIAQLVEHGLVRPSFVPPRPIRELRELTRYRKTQIQERSREVQRLDKVLQDAGIKL
                     SSVATDIIGVSGRAMLEALVAGTHDPEVLAALAKGRLRTKLPALRAALAGRFRTDHHG
                     LLVAQILAHIDYLDEAIAVLSARIQQVIAPFAEQVALLDTIPGVDQRAAEVILAEIGP
                     DMGQFPTAAHLASWAGVCPGNNESAGKHRSGRTRKGSKWLGGCLSEVAKAASRTKGTY
                     LNAQYHRLRGRRGPGKATMAVAHSVLVIAYHLLDQGVAYQELGDDYFQQRHSAERYQR
                     QLVRQLERLGHKVTLEPIEAA"
BASE COUNT          304 a          625 c          618 g          259 t
ORIGIN      
        1 gcccaccaag aagtcccaga agaaggcggc caagcctgcg aaggccgcct gaccggcacg
       61 gtccaccctt cacgcccgcc gacctcggtc gacggccaac tcgcgacctc cccgggttaa
      121 atcacaagct aagaacgcat accagtggcc tgattgagcg catacctttg gcccgtacgg
      181 tcggccgcga cctgggaaac ggcggtccgc acagcggtta ccggcattag ctgataagcc
      241 tcccatggcc gtggccggcc acccgcgacg gatgaaaatg tgccggtcat ccggccgacg
      301 tcgccgctct cttggtgccg agagcccgct gataagtctg acgtgggagc ctcgggcacc
      361 ccgcactgtt gccacgagca cgagactcag attcccctga tagaggctcc tccggatggc
      421 agcgatccgg tcgccgaggt ggaggaggcg agcaggtgga gacgctggtc gaacgctgcg
      481 ccggtctgga tgtgcacaag gactcggtga ccgcctgtgt acgggtaccc gacggccagg
      541 gtagacgcca ggccgagacc cgcaggttca ctacgacgac cgccgggctg gggctgctgg
      601 cccagtggct ggccagcttc ggggtgaccc gggtggggat ggagtcgacc gggtgctact
      661 ggaagccagt ctggcagctg ctcgagggcc agatggagtg ctggctgcgg aacgcggccc
      721 acctgcacaa cgtgcccggc cgcaagaccg acgtggccga tgcggcctgg atcgcccagc
      781 tggtcgagca cggcctggtc cggcccagct tcgtgccgcc ccgcccgatc cgggagctgc
      841 gcgagctgac ccgctaccgc aagacccaga tccaagagcg cagccgggag gtccagcggc
      901 tggacaaggt gctgcaggac gctgggatca agctgtccag cgtggccacc gacatcattg
      961 gcgtctccgg ccgggccatg ctggaagccc tggtggccgg cacccacgac ccggaggtgc
     1021 tggccgcgtt ggccaagggc cggctccgga ccaagctgcc agcgctgcgg gcggccctgg
     1081 ccggccggtt ccggaccgac catcacgggc tgctggtcgc ccagatcctg gcccacatcg
     1141 actacctgga cgaggccatc gcggtgctgt cggcgcggat ccagcaggtg atcgccccgt
     1201 tcgctgagca ggtggcgctg ctggacacca tccccggagt cgaccagcgc gccgccgagg
     1261 tcatcctggc cgagatcggc cccgacatgg gccagttccc caccgcggcc catctcgcct
     1321 cctgggcggg ggtgtgtcct gggaataacg agtcggccgg caagcatcgg tcggggcgga
     1381 cccggaaggg ttccaagtgg ctgggtggct gcttgagcga ggtcgccaag gcggccagcc
     1441 ggaccaaggg cacctacctg aacgcccaat accaccggct gcggggtcgc cgcggcccgg
     1501 gcaaggccac catggccgtg gcccactcgg tgctggtgat cgcctaccac ctcttggacc
     1561 agggcgtggc ctaccaggag ctgggcgacg actacttcca gcagcgccac tccgccgaac
     1621 gctaccagcg gcagctggtc cgccagctgg agcggctggg ccacaaggtc accctcgaac
     1681 ccatcgaggc ggcatgacca gccaacgcga ggtagcgcct gccctcgggc attttcgact
     1741 caggatgtgc tctccgctgg cgcgcaaccg gttggcctgc caaaccaatg tcgtccacct
     1801 gtgatg
//
LOCUS       DATADN010000944         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1179165, whole genome shotgun sequence.
ACCESSION   DATADN010000944 DATADN010000000
VERSION     DATADN010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179165"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VHH53_12240"
     CDS             <1..285
                     /locus_tag="VHH53_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290933.1"
                     /translation="DLDRRHLWQACETFATRHRRFGTATATVPLDALVRGDWQVADAA
                     PAAACREGSGLVAAHLQWHLERQLRSLPLVERATPASPHSAGSAGLSGGQ"
     gene            328..1113
                     /locus_tag="VHH53_12245"
     CDS             328..1113
                     /locus_tag="VHH53_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX2290934.1"
                     /translation="MVRAPDPHPSGARPPLLPANRMPRHVALVMDGNGRWAQQRGLSR
                     TEGHRRGEAVLLDVVCGCIELGVTWLSAYAFSTENWRRSPEEVRFLMGFNRDVVRRRR
                     DQMHEMGVRVRWAGRRPRLWRSVIRELEAAEELTAGNEVMTLTMCVNYGGRAEIADAA
                     REVARRVARGEIDPERVDERMLARHLDEPDMPDVDLFLRPSGEQRISNFLIWQSAYAE
                     LVFQDVLFPDLDRRHLWQACETFATRHRRFGTATATVPLDAQT"
     gene            1127..1552
                     /locus_tag="VHH53_12250"
     CDS             1127..1552
                     /locus_tag="VHH53_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290935.1"
                     /translation="MSTDKAVTSLACTRELLDAARTALERYQDVKVDDDGALSFQHSD
                     VPCAVQAVELAEGLLVLSLTCVVAWDVSDDGQLAERVATRAGQGLFGTLGLVRGQRGA
                     DVMLRYTFPAAGLDTVPLGTLLMLVVSTASQVRNELLAG"
     gene            1552..2670
                     /locus_tag="VHH53_12255"
     CDS             1552..2670
                     /locus_tag="VHH53_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX2290936.1"
                     /translation="MAAPPAAPRTAPDARTPPWRRITSMEVLCAVLVLALMAQGPLIR
                     LLDIAAVRTGATVFVAVCVQAMPFLVLGVLVSGLIAAFVPPAALRRMLPRSTVAAVPV
                     AGVAGMALPGCECASVPVSRRLMQQGVPPAAALTFLLAAPAVNPVVLVSTAVAFPGEP
                     RMVLARFLGSMATAVVMGWLWARFGRLEWIAQRALRRPQRDPGRSAVAVFTDTARHDL
                     VSSGGFLVLGGLTAAALNVLVPPSWMASLAGQMLLGIVVMALLAVVLALCSEADAFVA
                     ASLSALPLLPRLVFLVVGPAVDVKLVALQTGAIICPVQLDEPTWRSIGDVDPELAQQV
                     RERLFRELEDGTTLGAGAHFPELQFGRVIAGQGRRWFT"
BASE COUNT          374 a          966 c         1027 g          490 t
ORIGIN      
        1 gacctggacc gcaggcatct ctggcaggcc tgcgaaacct tcgccacccg tcaccgccgg
       61 ttcggcaccg ctaccgcgac cgtgccgctc gacgccctcg tgcgtggaga ctggcaggtg
      121 gccgacgccg cgccggccgc ggcctgccgt gagggcagcg gcctggtcgc cgcccacctc
      181 cagtggcacc tggaacgcca acttcgctcg ctacccctgg tggagcgagc aaccccggct
      241 tctccacatt cagcaggctc agcggggtta tcgggcggtc aataaccccg ctgagcctgc
      301 tgaatgcgat gacggcagga tggggcggtg gttcgagcac ctgatccgca tccctcgggt
      361 gcccggccac ccctgctacc tgctaatcgg atgccccggc atgtcgcgct ggtgatggac
      421 ggcaatgggc ggtgggccca gcagcgcggg ttgtctcgca ccgaggggca tcgccgtggc
      481 gaggccgtct tgctggacgt ggtgtgtggc tgcatcgagc tgggtgtgac ctggctgtcg
      541 gcctatgcct tctccaccga aaactggcgg cgtagtcctg aggaggtgcg cttcctgatg
      601 gggttcaacc gtgacgtcgt tcgccgccgc cgcgatcaga tgcacgagat gggggtacgg
      661 gtgcgctggg cgggtcgccg tccgcggttg tggcgcagcg tgatccggga gcttgaggcc
      721 gccgaggagc tgaccgcggg caatgaggtc atgacgctga ccatgtgcgt caattacggc
      781 ggtcgcgcgg agatcgccga cgcggcgcgt gaggtggccc gccgggtggc ccgcggtgag
      841 atcgacccgg agcgcgtcga tgaacggatg ctggcccgcc acctcgacga gccggacatg
      901 cccgacgtcg acctgttcct gcggccctcc ggggagcagc gcatctccaa ctttctgatc
      961 tggcagtctg cctacgcgga gctcgtgttc caggatgtgc tgtttcccga cctggaccgc
     1021 aggcatctct ggcaggcctg cgaaaccttc gccacccgtc accgccggtt cggcaccgct
     1081 accgcgaccg tgccgctcga cgcccagacc tgaggaggac gatccggtga gtaccgacaa
     1141 ggcagtgacg agccttgcct gcacccgtga gctgctcgat gccgcccgga cggcattgga
     1201 gagataccag gacgtcaagg tggacgacga cggtgcgctg agcttccagc attccgatgt
     1261 gccctgcgcg gtgcaggcgg tggaactcgc cgagggattg ctcgtcctga gcctgacctg
     1321 cgtggtcgcc tgggacgtca gcgacgacgg tcagctggcc gagcgggtcg ccactcgagc
     1381 cgggcagggc ctgttcggca ccctgggatt ggtccgcggc cagcgcggcg ccgacgtcat
     1441 gctgcggtac accttccccg ctgccggcct ggacaccgtg ccgctgggca cgctgctcat
     1501 gctggtggtg tccacggcct cacaggtgcg taacgagtta ctggccggct gatggcggcg
     1561 ccacccgccg ccccccgcac cgctccggac gctcggaccc cgccgtggcg ccggatcacc
     1621 tcgatggagg tgctctgcgc ggtcctggtg ctggcattga tggcccaggg accgctgatc
     1681 cggctgctcg acatcgcggc ggtccgcacc ggagccacgg tcttcgtggc ggtgtgcgtg
     1741 caggcgatgc cgttcttggt gctcggcgtg ctggtgagcg ggctgatcgc ggcgttcgtg
     1801 ccgccggctg cgttgcgccg gatgctgccg cgatcgaccg tggccgcggt gcccgtcgct
     1861 ggggtggccg ggatggcgtt accgggctgc gagtgcgcat cagtaccggt gtcccggcgg
     1921 ctcatgcagc aaggagtgcc gcccgccgcg gcgctgacgt tcctgctcgc cgcaccggcg
     1981 gtgaacccgg tggtgctggt gtccaccgcg gtcgccttcc cgggggaacc ccggatggtg
     2041 ctggcccggt tcctcggctc gatggccacc gcggtggtga tggggtggct gtgggcgcgc
     2101 ttcggacgtc tggagtggat cgcgcagcgg gcgctgcgcc gtccccagcg agacccgggg
     2161 cgcagcgccg tcgcggtgtt caccgacacc gcccggcacg acctggtctc ctcaggtggg
     2221 ttcctggtac tcggcgggct aaccgcggcc gcgctgaacg tcctggtacc accgtcgtgg
     2281 atggcctcgc tggccggtca gatgctgctc gggatcgtgg tgatggcgct gctcgcggtg
     2341 gtgctcgcgc tgtgcagtga ggctgacgcg ttcgtcgccg cttcgctgtc ggcgttgccg
     2401 ttgctgcccc gcctggtatt cctcgtggtg gggccggcgg tggacgtcaa gctggtggcg
     2461 ctgcagaccg gcgcgatcat ttgcccggta cagctcgacg aacccacctg gcgatcgatc
     2521 ggcgacgtcg atcccgagct cgcacaacag gtccgcgaac gactgttccg cgagctcgag
     2581 gacggcacaa ccctgggcgc cggcgcacac ttccccgagc tgcagttcgg ccgcgtcatc
     2641 gccgggcaag ggcggcgctg gttcacctga ccgtgaggcc gaagcggttt acacaacgct
     2701 cgcgcgagaa caaaggcacc gatgtcgaac tgcctccccc gcagcctcgg atctcacgtt
     2761 ccggcgggat ccggcggtgc cgggccttgg ccacggtgcg gacggcacat cctcactgtc
     2821 aagcgtcacg tgggaccaga caccgtcgag ggtcgct
//
LOCUS       DATADN010000945         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_257198, whole genome shotgun sequence.
ACCESSION   DATADN010000945 DATADN010000000
VERSION     DATADN010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257198"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VHH53_12260"
     CDS             complement(<1..748)
                     /locus_tag="VHH53_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2290937.1"
                     /translation="MPPRHHHGIFAMMYRLPEFSIQGDQLAFMQHAHVARRVLEVGKW
                     LALGRALPVPVSLHPREGARAVPAVTGRYVPVTVGGVTYQIYTESAGSGQDVLCMHTA
                     GADGRQFHGLMADPRVVEHHRLVSFDLPWHGKSPLPEGAIQGSWRLNTDLYVSLIMGF
                     IAAVGLDKPVALGASMSGEICLELAYRHPEAFAGIIACEACDKINQRQTVWAAHPHVN
                     QAQFVPEWIRALSAPQSPAEYVEQITWHYGQ"
     gene            complement(1015..2064)
                     /locus_tag="VHH53_12265"
     CDS             complement(1015..2064)
                     /locus_tag="VHH53_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2290938.1"
                     /translation="MKVRFGVSVISGTAFPHRRDLDKFAELIRIIDGSGVELIGTNDT
                     SFIGGDAYVRATLIAQTARNAQVGIHPTNPLTREPQIMAAFLGSIDAMTEGRAFLDIG
                     SGDSAVYNIGLAPASRARLADYVTCVRDLVATGEGTYDGRPQRVRWHGEAVRNRIPIT
                     LCAEGPRTLHLGGRLFDGVIAGTGLLPEVISDTIERVAAGARTAGRDPSQVEVWFTTR
                     SSLDADRDKAIERIHASVSSILNHSMRFGLEGKNVPTQFRAKIAEYVKGYELYDHVLQ
                     AGRNPKRMAELGLTEYGLERFALAGNLNDWIERIGALAELGASRLWLSTEAGDLDRQI
                     HYMKVFTEQIMPHFR"
BASE COUNT          397 a          729 c          722 g          374 t
ORIGIN      
        1 cctgaccata gtgccacgtg atctgttcca cgtattcggc cgggctttgc ggggcactga
       61 gggcccgtat ccactccggc acgaactgcg cctggttgac gtgcgggtgt gccgcccaaa
      121 cggtttgacg ctggttgatc ttgtcgcaag cctcgcaggc gatgatgccg gcgaaggcct
      181 cgggatggcg gtaggccagt tccaaacaga tctcccccga catcgacgcg ccgagggcca
      241 ccggcttgtc cagtcccacg gcggcaataa agcccatgat cagggagaca tagaggtcag
      301 tattcaggcg ccaggaaccc tggatcgcgc cctccggcag cggcgatttg ccgtgccagg
      361 gcagatcgaa cgataccagc cggtgatgtt cgacgacgcg cggatcggcc attagaccgt
      421 ggaactgccg cccatccgcg ccggcggtat gcatgcacag cacgtcctgc cccgatcccg
      481 ccgactcggt gtagatctga taggtcaccc cgccgacggt cacgggcacg tagcggccgg
      541 tgacagcggg caccgctcgg gcaccttccc gggggtgcag cgacaccggc accggcagcg
      601 cccggcccag ggccagccac ttcccgacct cgagcacccg ccgcgcgaca tgggcgtgct
      661 gcatgaaggc gagttggtcg ccttgaatgg aaaattccgg cagccggtac atcatcgcga
      721 agatgccgtg atggtgacgc ggcggcacgg gttcgaggaa ccgcgcccat gtcgcctcgg
      781 gtgccgccag ggtgaaggcg ggagtacccg ggtcgtcggc gatctgtcct ccgacgaaat
      841 tgaaaatcgc cgtgtcgctt cccgacctga ccgcgaaaca aacagcccag ggcccggccc
      901 aggtggcgag gaccgcatcc gcagcgcaag ccttgtgcca agcggtggta ttcggaaacc
      961 cggtcattgc atcagtcact tgcggaacta cgggatacca gcggtcgcag cgagctaccg
     1021 gaaatgcggc atgatctgtt cggtgaacac tttcatgtag tggatctgcc ggtcgaggtc
     1081 gccggcttcg gtgctgagcc agagccgcga ggctcccaac tccgcgagcg cgccgatacg
     1141 ctcgatccag tcgttgaggt tgccggcgag cgcgaaccgc tcgagcccgt attcggtcag
     1201 cccaagctcg gccatccgct tcgggttgcg cccggcctgg agcacgtggt cgtacagctc
     1261 gtaacctttg acgtactcgg cgatcttggc ccggaactgg gtcggcacat tcttgccttc
     1321 gagaccgaac cgcatcgaat ggttgaggat cgacgacacg gatgcgtgga tgcgttcgat
     1381 agccttgtcc cggtcggcgt cgagcgacga gcgggtcgtg aaccagacct cgacctggga
     1441 gggatcgcga cccgccgtcc tggcacccgc cgcgacgcgc tcgatggtgt cagagatcac
     1501 ctcgggcagc aggccggtgc cggcaatcac gccatcaaag agtcggccgc ccagatgcaa
     1561 ggtccggggc ccttcggcac agagggtgat cgggatgcgg ttgcgcaccg cctcgccatg
     1621 ccaacggaca cgctgtggac gaccatcgta cgtgccctcg ccggtggcaa ccaggtcccg
     1681 cacgcaggtg acgtaatccg ccagccgggc gcggctcgcc ggcgcgagac cgatattgta
     1741 gacggcgctg tccccgctgc cgatatccag aaaggcgcgg ccctcggtca tcgcgtcgat
     1801 cgatccaagg aacgccgcca tgatctgcgg ctcgcgggtc agcgggttgg tcgggtggat
     1861 cccgacctga gcattcctcg cggtttgcgc gatcagcgtc gcgcggacat aggcgtcacc
     1921 accgataaag ctggtatcgt tggtgccgat cagttcgacc ccggacccgt cgatgatccg
     1981 gatcagctcg gcaaacttgt cgaggtcccg ccggtgggga aacgccgtcc cgcttatcac
     2041 cgacacacca aagcgaacct tcattacctc tcctgtcagg caactgcagg atcagccggt
     2101 gtcggcggag gatccggcgg ttcggcaccc agccgacgcc gctctccgct tgcccggccc
     2161 gatcaactga ggctgccggg atagtcctgc cagatgctcg gttcgagatg caaatgcgta
     2221 ct
//
LOCUS       DATADN010000946         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_426084, whole genome shotgun sequence.
ACCESSION   DATADN010000946 DATADN010000000
VERSION     DATADN010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426084"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1504)
                     /locus_tag="VHH53_12270"
                     /pseudo
     CDS             complement(280..1504)
                     /locus_tag="VHH53_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072274.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1912..2111)
                     /locus_tag="VHH53_12275"
                     /pseudo
     CDS             complement(1912..2111)
                     /locus_tag="VHH53_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          289 a          596 c          813 g          413 t
ORIGIN      
        1 gcgctggccg catctgtgcc gtgctggcca gagcgcgcac ctcactatac cggcggcatc
       61 ggtgctgcgg acagtcgctt cgccggtgtg gtcaccctcg gtctaaccac gtagaatttc
      121 ctcgccaaat gatctgcatt attaccattg gacggttacc ggcactgatg cccctatgga
      181 tgtcaagctg ctgaggccgg ctgggtagtg cgttcggggc tgggcttgag gagttggtag
      241 atttcgcggg cgatgtagac atcccatgcg gtcaagttgt caagctgcgg tttgctggct
      301 ggtggtcgtg gtggttggga aggcgatgtt ttcgttgtag tgctggcggg tttcgaggca
      361 gtggtggagg cagccgatga ggcggttgaa caggttgcgt tgggcggcga cgtggtggtt
      421 gccggtggtt ttgcggcggt cgtagtgggc tctggcgccg ggtgaggcgg tcagcgcggc
      481 gaaggcccag aggtagccgg tggcggcgag tcgttggttt ttgacctgtc ggtggatcac
      541 tgctagggac ttgccgctgg cgcgggtcac gggggcgctg ccggcatagg ctttaagcgc
      601 tttggcgtgg ctgaagcggg attggtcgtc gccgatctcg gcgagcaccc gggcaccggt
      661 gagcgcaccc agatcgggct ggctggtgat gattggtgcg tcggggtggg tgtcgaagtg
      721 ctcgatcacg gcgcaggcaa gctcgtcggc gctggtggcg gccgcgttga gttggtggtg
      781 cagggcgagg gcttggcggc cgaaggcgtc ttcgactagc ggtagctggt gcaggtaatc
      841 ggcgcgcagt attgcctgga gccggtcggc ttcggcgtcg atggcgcgtt gtcggccggc
      901 gcgtcgcagc agcgcccgca gttgggttgt ggtcggcttc gcggccgcgc gcggggtcgg
      961 ggcggcggcc aggacggcgc gggcgtcggg gcgcagcagc ccgtggcgtt tgtcggcgaa
     1021 ggcggcgatg atccccgggt agtactcgcg cagttgcgaa cgcagcgtgt tgtgtgcgca
     1081 ggtgcggtcc cagacggcgt cttgctgtgc gcgggcgagc acggcgatgg cctgggcgag
     1141 ttttggaatc ggccggcagc ggccggtggg agggtaggtc ggtgcgcagg atgttcgcca
     1201 gcacgagtgc gtctccggcg tcggatttct tgcgcgccac cgagtgccgg tcccggtagc
     1261 gggataccgc catcgggttg atcgcgaaca ccttgcggcc ggtggcccgc aggcaggcga
     1321 ccagcagacc gcggccggtc tcgatggcca ccgggatcgg ttcctcggcg ctgtcgccgg
     1381 catcggcgag cacctgcaac agcgcggcga acccggccgc gtcgtcgctg atccggcgct
     1441 tggcgatcag cttgccctcg gcgttgacca gggtgacgtc gtgccggtct tgcgcccagt
     1501 cgatgccaca gacactgtca agatttcgtt cctcctgtcg tgagctgcct gttcatgcgg
     1561 tccaaagctc ggggcaggga cacgcgagcg ccctaatggc agggctcgaa ggcccgacat
     1621 cccaccagcc gttcgtgacc ccagcggacc gcacggctct ccgtcttcgg aggagctcaa
     1681 gggctcacgt cttagtcaga ggtgatccgt gcgggcggct tgaaccacga cgtcaacgac
     1741 agcgcggcgt ggccgtcacg gtcaggcggc gcgaggcaga aataccagct aggtgccgcc
     1801 cggccatgac atgcacgcga gaacgaggcg aaacgctccc gtctacatca aaggctcacg
     1861 gccttgcgct ttagtaggag tccctcccgt ctcggcgttg cctgcccatt agcgcttgag
     1921 gttgcggatg gcttcgcggc gggttttgcc tttggtgatg cggcgggcga ggtagtcgcg
     1981 ggtgcgagcg tcccagcgca gtcgtgacag ggcgatacgg tagagcgcgg aatttgcttg
     2041 tcggtcgccg ctacggttaa gctggcgacg gctggtcctg cctgaggacg cctccagggg
     2101 gctgacaccg c
//
LOCUS       DATADN010000947         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_257350, whole genome shotgun sequence.
ACCESSION   DATADN010000947 DATADN010000000
VERSION     DATADN010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257350"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(594..1094)
                     /locus_tag="VHH53_12280"
     CDS             complement(594..1094)
                     /locus_tag="VHH53_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290939.1"
                     /translation="MDFNGHGRVFIGAHPTGGVIGFWQPAAPWTFRTTTPGALLWAEL
                     NTWHGALADKIFADLFSYHHAQIGDGVDLDYTTWSRGGQMTLGRLQMNEDWAAPETRA
                     HWTLHFAVDPQTGTDATVDRVLEMGGRVDIDPYDSEWATSRGSPSPWGPALRSSTPLR
                     GSWRRT"
BASE COUNT          366 a          607 c          540 g          337 t
ORIGIN      
        1 acccgaatca cgaatccggt tattggccgc cactgagcac tgtccagcaa tgccgtaccc
       61 cgggcagccc cagggtcatc cacaccaaac ccggccgcag gaaggcgccg aattttgctc
      121 aatctcaccc acagattgca aggtcaggtc agggcgttca ccggccggta agccagacga
      181 tgccgacaaa gtacgcgcag cctaagatta acgctgtcac ggccaccgcc cagttcatcg
      241 tgcgctcgcg tcctgggccg acagcccagt ttggtccatg atcctctcct gattcagtat
      301 ctattacata tcggatccat gagcgaaact tgatttgaac aaaatatact ttcgagagtc
      361 cgaaatcaag acatgccggt caaatgagga tcgcccagca cgctcatccg catttgcatt
      421 tgctcgacca taaacgcacg ctcagtcgcg gttgcatatt ctcacttgcg tgcgtccgtg
      481 cccgcattat tggcgtaaag ccccttatcc acccggtaca cgtcacggcc agagggttcc
      541 cgccttgccg agtcagtcgt cgtagggatc gtccacgcgg gccgaaccgg ccgctaggtc
      601 cgccgccacg atccacgaag tggggtcgat gagcgcaaag cgggccccca ggggctcggc
      661 gacccgcgcg atgtggccca ctcgctgtcg tagggatcaa tgtcgacccg gccacccatc
      721 tccagcaccc gatcgaccgt cgcgtcggtg ccggtctgtg ggtctacggc aaagtgcagt
      781 gtccagtgcg cacgggtttc gggggcggcc cagtcttcgt tcatctggag tctgcccaac
      841 gtcatctgcc caccccgaga ccaggtggtg tagtccaggt cgacaccatc cccgatctgc
      901 gcatggtggt aactgaacaa gtcggcgaag atcttgtcgg ccagcgcgcc gtgccaggtg
      961 ttcaactcag cccacaacag cgccccgggt gtggtcgtgc gaaacgtcca cggtgccgcg
     1021 ggctgccaaa aaccgatcac gccgccggtc ggatgcgccc cgatgaatac cctgccgtgc
     1081 ccgttgaaat ccaccggtcc ctgcagcact tggccgcccc actgtgtaag tactgcggcg
     1141 gtgtgggtga tgctgcgctg gtcagataca cagtccaggt cactggttta cccgccggca
     1201 ttggaacgcc cgccaggccg gccaccggcc agccagcaca cagggctatg gcgtggcgcc
     1261 cgcgacccgg gttgatccgg taagtccagc cgaacaacct aaaaatctcg actaccagcc
     1321 ggatcggtgc tcgacacgtc gatccaacac accgaaccgg ggataaatga cgtgcccgtg
     1381 tccagccacg aggaatgcgc cttggtcgtc cgcatgtctt aacagaggcg ccccacgccc
     1441 gtgcaaatac acgctcagga caaggcgagg atctcgggcg gcatggcggc gagctagtta
     1501 gcaccaccac tgcgaaaccg cctcatccag ttggcgtccc cgttgggcgt cgatggctga
     1561 ggcggtccga ggctcggctg cctgatcaag ggatcatgat gtttggactg cctggtgggt
     1621 gatgacaccg cggcgcacgc ctcgcacggc ttcccattgg gcgcctggtg caaggtggtg
     1681 accatcatca gcaagtgtcc gcacacagca ttgtagatgc ccaacggagt ggtagcggtt
     1741 accattgccg gctctgtcga gccgctagcg cccgcagtaa ggcgacgtgg gtggcccgaa
     1801 cctcatcgac cagcgatatg gcccagtcct gggcggcatc ctgttccaag
//
LOCUS       DATADN010000948         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_572671, whole genome shotgun sequence.
ACCESSION   DATADN010000948 DATADN010000000
VERSION     DATADN010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572671"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="VHH53_12285"
     CDS             complement(<1..1852)
                     /locus_tag="VHH53_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2290940.1"
                     /translation="MDEQWSRGRTVTRLATAGLAAVLLLLTGFALWAAFSTNRAARQA
                     DRLHRLSDAYQEARFAVAVEESLERKYRLEPGPKIRQRFQQAAASLLAALDGVRKRGD
                     ANDRALAAKVEAEHARYLDGIDRMFNAVDAGNTALVLAIDGAETEPAFAAIEAQVNAA
                     ADAHHEQALASLRALRNTGAMVLVATPVAFAAGLGLLAMFWAIVVGYQRRTERQAAET
                     KRQAAENQHQALHDALTGLPNRALLRDRIDQAIHQADRELIPAALLLLDLDRFKEVND
                     TLGHHYGDQLLVQVGERLRDRLREVDTVARLGGDEFAVLLPRIETAEGAVAVAGKLQA
                     AFDEPFMLDDLALDVEASIGVALYPEHGSDPDELLQHADIAMYVAKDSHAGFMVFDPK
                     QDQHSPRRLALLGELRRAMEQQQLVLHYQAKVDAHTGQMLGVEALVRWQHPEHGLLSP
                     GDFIPLAERTGLIGPLTHYVLDTALHQCHQWRDAGHELSVAVNVSARSLLDLAFPDQV
                     ASLLARWDLPARLLVVEITESTIMADPTHALEILSRLNIMGVQVAIDDFGTGYSSMAH
                     LKTLPVHELKIDRSFVSQMTSYTRDAVIVRSTVDLARNLGLRVVAEGVEDA"
     gene            complement(2271..2798)
                     /locus_tag="VHH53_12290"
     CDS             complement(2271..2798)
                     /locus_tag="VHH53_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2290941.1"
                     /translation="MMTPSDGSSPDAQTLSSTARLREIADLGLNDSDIDGILSEIAAE
                     AAAHFGLPVALVSIVLDQAQYFRGSHGLDGSMATAGGTPAEWSFCQHVVRNRAPFIVE
                     DAQHHPLMQDSPLVRIEGIRCYAGVPLITFRGHVIGSFCVQGPDARSFTKYDLAQLQR
                     YAKRVITRIEQRRRG"
     gene            complement(2795..3082)
                     /locus_tag="VHH53_12295"
     CDS             complement(2795..3082)
                     /locus_tag="VHH53_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290942.1"
                     /translation="MTTDQPSATVYGLTLPSEAEVIADLMRLVSADVAIFASREASDA
                     DEEAGPRGYVELLLRLGEQLAARSDVAGVVGRSLAVRVNTYIALSSPRTSA"
BASE COUNT          532 a         1064 c         1158 g          571 t
ORIGIN      
        1 ccgcgtcctc aaccccttcg gcgaccaccc ggaggccaag gttgcgggct aggtccacgg
       61 tcgagcgcac gatcaccgca tcgcgagtgt agctggtcat ctgcgacaca aacgaccggt
      121 cgatcttgag ctcgtgcaca ggcagggtct taagatgggc catcgaggag tagccggtgc
      181 cgaagtcgtc aatggccacc tgcacaccca tgatgttgag ccggctaagg atctccaggg
      241 catgggtcgg gtcggccatg atggtgctct cggtgatctc caccaccagc agccgcgcag
      301 gaaggtccca gcgggcgagc aggctggcga cctggtcggg gaaggcgagg tcaagcaggc
      361 tgcgggcgga tacgttgacc gcgaccgaca gctcgtggcc ggcgtcgcgc cactggtggc
      421 attggtgcag cgcggtgtcg agcacgtagt gggtaagggg gccgatgagc ccggtccgtt
      481 cggccagggg aatgaagtcg ccgggggaaa gcaggccgtg ctcagggtgc tgccagcgca
      541 ccagcgcctc cacccccagc atctggccgg tgtgggcgtc gaccttggcc tggtagtgga
      601 gcaccagctg ctgctgctcc atcgcgcggc gcagctctcc gagcagggcc agccgccgcg
      661 gactgtgctg atcctgcttg gggtcgaaca ccatgaagcc ggcgtggctg tccttggcga
      721 cgtacattgc gatgtcggca tgctgcaaca gctcgtcggg gtcgctgcca tgctcgggat
      781 aaagggctac accgatgctg gcctccacgt ccaaggccag atcatccaac atgaacggct
      841 cgtcgaaggc ggcctgcagt ttgccggcga cggccacggc accctcggcg gtttcgatgc
      901 ggggcagcag cacggcgaac tcgtcgccgc ccagccgggc taccgtgtcg acctcacgca
      961 acctgtcccg caggcgttcg ccgacctgga cgaggagctg gtcgccgtag tggtgcccga
     1021 gggtgtcgtt gacctccttg aaccggtcga ggtccagcag cagcaacgcg gccgggatca
     1081 gttcgcgatc ggcttgatgg atcgcctggt cgatgcggtc gcgcagcagg gcgcggttgg
     1141 gcaggccggt gagtgcgtcg tgtagcgcct ggtgctggtt ctcggctgcc tggcgcttgg
     1201 tctcggccgc ctgccgctcg gtacgacgct ggtagccgac cacaatcgcc cagaacatcg
     1261 ccagcagccc aagaccggcg gcgaacgcga ccggggtcgc caccagcacc atggcgccgg
     1321 tgttgcgcag tgcacgcagg ctggccaggg cctgctcgtg gtgggcgtcg gcggctgcgt
     1381 tcacctgcgc ttcgatcgct gcgaacgccg gttcggtctc ggcgccgtcg atcgccagca
     1441 ccagcgccgt gttaccggca tccaccgcat tgaacatgcg gtcgatcccg tccaggtagc
     1501 gggcatgctc agcctccacc ttggccgcca gagctcggtc gttggcgtcg ccccgcttcc
     1561 gtacgccgtc gagcgcggcc agcagcgagg cggcggcctg ctggaaccgt tgccggatct
     1621 tggggcctgg ctccaggcgg tacttgcgct ccagcgactc ctcaactgcg accgcgaagc
     1681 gagcctcctg gtacgcgtcg ctcaggcggt gcagccggtc ggcctgacgg gcggcccggt
     1741 tggtgctgaa ggccgcccac aacgcaaacc cggtcaacaa cagcagcacg gcggccagcc
     1801 cggctgtcgc caaccgggtg acggtccggc ccctagacca ctgctcatcc acgcggcctt
     1861 ctccctgctc cgaatcgcgg cggttcgcga tcgggatctc cgccaatccg ctgagcgttc
     1921 ggtaatgaat tcggcacacg agctgcgaac ctaagctaag cgcacagcgc atcctcggag
     1981 agaccatctc caggcgtggc tagcgcacct atccgacgga gcaggtgtgt cgttggcagc
     2041 gatttggagc ggctcttgcc gtggcttggc ggatacatgg cgacgttggc tcgtcgcagc
     2101 acgagccaac tggcccagga ctgcgatcag gtcgggcgcc cgttcggagg cgcgctgagg
     2161 agctgtgcgc tgctgtggtc gagcaccgca atgtgactat ttgcggtgaa cgctcacgcc
     2221 gatacacgtc atgaggaatc ttcttgtacg agcgtatggc tcggccccac tcagccccga
     2281 cggcgttgct caatgcgtgt gatcactcgt ttggcgtagc gctggagctg ggcgaggtcg
     2341 tatttggtga agctgcgggc gtcgggcccc tgaacgcaga acgaaccgat cacatgcccc
     2401 ctgaaggtaa tcaaggggac gccggcataa cagcggatcc cctcgatgcg gaccaagggg
     2461 ctgtcctgca tcaacggatg gtgttgcgca tcttcgacta tgaacggtgc acggttacga
     2521 acgacgtgtt ggcagaagga ccactcggca ggggtaccgc cggccgtggc catcgagccg
     2581 tccaggccgt gcgacccgcg gaagtactgg gcctggtcga gcacgatgct gaccagcgcg
     2641 acgggcaaac cgaagtgcgc ggcggcctcg gctgcgatct ccgagaggat gccgtcgatg
     2701 tccgaatcgt taaggcccag gtcggcgatc tcccgtaggc gggcggtgct ggagagcgtt
     2761 tgggcatcgg gcgacgagcc gtcggagggg gtcatcatgc tgaagtcctc ggcgaggaca
     2821 gcgcgatgta ggtattgacc cggactgcca gtgatcggcc gacgacgccc gcgacgtcgg
     2881 agcgggctgc gagctgctcg ccgaggcgca gcagcagctc gacgtagcct cgtggtccgg
     2941 cctcctcgtc tgcgtcggag gcttcccgcg aagcgaagat cgcgacgtcg gcgctgacga
     3001 gcctcatcaa gtcggcgatc acttcggcct ccgacggcag cgtcaacccg tagacagtgg
     3061 cgctgggctg gtcggttgtc acactcatgc tcctggtggc gtgccgctgc ggttggtggc
     3121 ctgctccgac gcacactgct cgtgctggcg taacctcttc atcggtaggt tgcttgcctg
     3181 gctgcagcat ggacggggtt gcgaagtggg cagggaccgt ccgagacccg gcgaggctca
     3241 acgggactgc agtcgcgcgc gccccgtccc tttcgtcggt gacggcccga tgttggacga
     3301 cgtcgtccga tggtccggta tcgct
//
LOCUS       DATADN010000949         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1479256, whole genome shotgun sequence.
ACCESSION   DATADN010000949 DATADN010000000
VERSION     DATADN010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479256"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..839)
                     /locus_tag="VHH53_12300"
     CDS             complement(9..839)
                     /locus_tag="VHH53_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2290943.1"
                     /translation="MSSDAQIWRRLQPVVAILLVAGVIGIPLWLVVVTAGKSQGEALN
                     PDLSLPSEWHLWGNLRQVWTEGEVPAAFFGSLLVVVPTVFGVLAFGSLASWVLARRAT
                     RLNAVLYALGISGIVLPPAVVTVVLLLRQLGLAGTAIGMIGVYMGIYMSTVIFFVTGF
                     VRTIPESLEEAAQIDGAGPLTVFRKVVLPMLRPVLATATILICLYVWNDVFYAFFVIG
                     GRIDTLPLNLFRVASAGLYLNNWHLIFAYVIVMSLPLVVIFAVAQRKIISGITGGAVK
                     "
     gene            complement(836..>1556)
                     /locus_tag="VHH53_12305"
     CDS             complement(836..>1556)
                     /locus_tag="VHH53_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2290944.1"
                     /translation="DNFSQLAANGTLFRSLRITILYAVLVALFQNVFGFGLAVLLERD
                     TLINRLARILFLLPVLMSALAVGYIFQALLKPDGSLNQLIGAVLRHPFEYPWLADTTW
                     TIVVVALIHAWKWMGLSMLIYLAGLKTQPEELIEAARIDGASRWQSFWRIRSPLLAPA
                     VTFNVATALLGSMNGFDVVQATTGGGPARTTEILNIFIYRTFGQGLFAQATTMSLMLF
                     LLVALMAFPVIYVLRRREAVL"
BASE COUNT          308 a          502 c          531 g          215 t
ORIGIN      
        1 ggccgaggtc actttacggc tccgccggtg attccggaga tgatctttcg ttgcgcgacc
       61 gcgaagatca cgacgagtgg caggctcatc acgatgacgt aggcgaagat gaggtgccag
      121 ttgttgaggt agagaccggc gctggcgacg cggaacaggt tcagcggcag ggtgtcgatg
      181 cgcccgccga tcacgaagaa cgcgtagaac acgtcgttcc acacgtacag gcagatgagg
      241 atggtggcgg tggcgagcac cggccgcagc atcggcagca ccaccttgcg gaacaccgtc
      301 agcgggccgg cgccgtcgat ctgcgcggcc tcctccagcg actccgggat cgtccggacg
      361 aaaccggtga cgaagaagat gaccgtcgac atgtagattc ccatgtagac gccgatcatc
      421 ccgatcgcgg tgcccgccag gccgagctgg cgcagcagga gcaccacggt taccaccgcc
      481 ggcggcagca cgatgccgct gatgccgagg gcgtacagca cggcgttcag ccgggtggcg
      541 cggcgggcca gtacccacga ggccaggctg ccgaacgcca gcacgccgaa cacggtgggc
      601 acgacgacaa gcaggctgcc gaagaaggcg gccggcacct cgccctccgt ccacacctgg
      661 cggaggttgc cccacaggtg ccattcggac ggcagtgaca ggtcggggtt cagggcctcg
      721 ccctgcgact ttccggcggt caccacgacg agccacagtg gaatcccgat gacgccggcc
      781 accaggagga tcgcgacgac cggctgcaac cgccgccaga tctgggcatc ggaactcaca
      841 gcacggcctc ccgcctgcgc agcacgtaga tcaccgggaa cgccatcagc gcgacgagca
      901 ggaacagcat caggctcatc gtggtcgcct gggcgaacag gccctggccg aaggtgcggt
      961 agatgaagat gttcaggatc tcggtcgtgc gggccggacc gccgccggtg gtggcctgca
     1021 cgacgtcgaa cccgttcatc gagcccagca gcgcggtcgc cacgttgaac gtgaccgcgg
     1081 gcgccagcag cggcgaccgg atgcgccaaa agctctgcca gcgcgacgcg ccgtcgatgc
     1141 gcgccgcctc gatgagctcc tccggctggg tcttgagccc ggccaggtag atcagcatgg
     1201 acaggcccat ccacttccac gcgtggatca gcgcgaccac cacgatcgtc cacgtcgtgt
     1261 cggccagcca cgggtactcg aacgggtgcc gcagcaccgc cccgatgagt tggttgaggc
     1321 tgccgtcggg cttcagcagg gcctggaaga tgtagcccac cgcgagggcc gacatcagta
     1381 ccggaagcag gaagaggatt ctggcgagcc gattgatgag cgtgtcgcgc tccaggagca
     1441 ccgcgaggcc gaatccgaac acgttttgga acagggcgac cagcaccgcg tacaaaatcg
     1501 tgattcgaag ggaacggaac agggtgccgt tggcggccag ttgactgaag ttgtcc
//
LOCUS       DATADN010000950         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1479308, whole genome shotgun sequence.
ACCESSION   DATADN010000950 DATADN010000000
VERSION     DATADN010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479308"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VHH53_12310"
     CDS             complement(<1..246)
                     /locus_tag="VHH53_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456331.1"
                     /note="Required in the synthesis of PPQ, but its exact
                     function is unknown; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein C"
                     /protein_id="HEX2290945.1"
                     /translation="MLRAQQHSYWDKHPFHQRMAAGELEPAHLRAYVANRWYYQRSLP
                     QKDAAIIANCPLPEIRRRWLPRISYHDGTTDGDGGCAR"
     gene            complement(323..1312)
                     /locus_tag="VHH53_12315"
     CDS             complement(323..1312)
                     /locus_tag="VHH53_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2290946.1"
                     /translation="MFVRILGSAAGGGVPQWNCGCQVCDATRSGSRPVTRRRSCTIAV
                     GDGAGGWFLGNAGPDLDAALGDCADLWPGTGQSTAPIHGVFLTDAELDHTSGLLSLRQ
                     AAELHLYATAAVRALLTHCGMLPTLESYTVIRWHEVRPGERFTLRYRDGSASPIWCEP
                     FDASCGRLPRYARGTVPSGRGDSQGDLPQGGDPGSGVVIGYRITDQRTARSVVFLPSV
                     AVLGDTALRHIDQAHLVLVDGTFWVDDEMSQHGRGTATARSMGHLPVSGADGSLRLLG
                     QPAGTRTGTPTGTQVVYTHINNTNPMLFEDGPERALLANAGASTAEDGAQYEL"
BASE COUNT          291 a          490 c          454 g          285 t
ORIGIN      
        1 gcgggcgcaa ccaccatcgc cgtcggtcgt gccgtcatga tatgaaattc gcggaagcca
       61 ccgccggcgg atctctggca acgggcagtt cgcaatgatg gccgcgtcct tctgcggcag
      121 gctccgctgg taataccacc ggttggccac ataagcccgt aaatgagcag gctcaagctc
      181 accggcggcc atccgctgat gaaatggatg cttgtcccag tagctgtgct gttgcgcccg
      241 caacattcct tcgaagtcgt cccggttcaa tgctggcagg ttttcatcca tctttcccct
      301 tccacttcgg ctcgtaccag gttcatagct cgtattgggc gccgtcttcg gcggtgctcg
      361 cgccggcatt ggccagcaac gcccgctcgg gcccgtcctc gaacagcatc ggattggtgt
      421 tgttgatatg ggtgtatacc acttgggtcc cggttggagt cccggttcgg gtgccagccg
      481 gttggccgag caagcggagg ctgccgtcgg caccgctgac gggcaggtgg cccatcgagc
      541 gggccgtcgc agtgccacgc ccgtgctggc tcatctcgtc atcaacccag aaggtgccgt
      601 ccacgaggac cagatgggct tggtcaatat gtctaagcgc agtatcaccg agcactgcga
      661 ccgacggcag gaacaccacc gagcgagcgg tgcgctggtc ggtgatccgg taaccgataa
      721 ccacacccga acccgggtcc ccgccttgtg ggaggtcgcc ttgtgaatcc ccacgccctg
      781 agggaacagt gccgcgagcg taacggggaa gccgaccgca gctggcatcg aaaggctcac
      841 accagatcgg cgacgcacta ccatctcgat accgcaacgt gaaccgctcg ccgggacgaa
      901 cctcatgcca acggatcacc gtgtagctct ccaacgtcgg gagcatcccg cagtgggtca
      961 gcagagctcg caccgccgcg gttgcgtaca agtgcaactc ggcggcctgt cgaagcgaca
     1021 gcagtcccga cgtgtggtcg agctcggcat cggtgagaaa aacgccatgt attggtgcgg
     1081 tggactggcc tgtaccaggc cataggtcag cacagtcacc cagtgcggcg tccagatctg
     1141 gcccagcatt gcccagaaac cacccgcctg caccgtcgcc aacggcgatg gtgcaagacc
     1201 ggcgccgagt cacggggcgt gagccagacc gcgtggcgtc acagacttgg catccgcagt
     1261 tccattgcgg tactccaccg ccggccgcag agcctagaat gcgaacgaac atctcaacgc
     1321 cagctggtta cactcagcgt ctaactaacc ttagcgtcga gccacgtaca tcgttacttc
     1381 catgggcgtc tctagccgtt cccaggttgg tgccacccac tgccggcgtg cacatggcgc
     1441 gccttgtgtg gattcctcaa gtggtccacc ggtatcaatg atgacacgac tttgttgacc
     1501 ggcaatagca gtcatatttg
//
LOCUS       DATADN010000951         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1479329, whole genome shotgun sequence.
ACCESSION   DATADN010000951 DATADN010000000
VERSION     DATADN010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479329"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VHH53_12320"
     CDS             <1..1441
                     /locus_tag="VHH53_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290947.1"
                     /translation="DNSRCGLSDAPPVFGLGRAYRLWTVPAPRPVDEGGRSSTTGSPA
                     VAERALRRAAGGSRFSASSPQAAELVQVLDTYRPAVTVVRALARRGRRAVVGREPDRR
                     HRAESAGAESSRHTTAVWDHPSIKDEPGAFLDALVHELARRPEPVHILPLNETALMLL
                     ARERTRLPANTSLAVPATETVVTCLDKQRCLNAAQAAGLSVPRWEVVGGYQELLAAAG
                     RAAAPVVVKPLWPHSSLLNDRKAILCRDGSDLARQIPVWPTGHDRLLVQRWAPGLRHD
                     LLFAARDGELLDMVEKLTVRTQRRDGTGLAVAGHTRPVSIGLRDQLAALVAHLRYTGV
                     GFGQFLVQPGAQDGAFLELNPRLSGSHVIADHVGLDLANLAVDLAADVPVTAHLPAPT
                     GRDGHTYAWTFGDLTGLWYAIGHSEITRAGALAELGRIARAAVLADVHVTWSWRDPLP
                     TIATCWRYLVLHSGTAAAASTSKRTAIDG"
     gene            complement(1382..>1739)
                     /locus_tag="VHH53_12325"
     CDS             complement(1382..>1739)
                     /locus_tag="VHH53_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290948.1"
                     /translation="SVFADVPALSANSPNKVFDAFAASRPVVINNGGWLAEVLRESGA
                     GIAVPPDDPVAGAKMIAQHITDSDFLARARSAAGVLARGQFNRDVLFEKFEEVLLSAV
                     NRGALRGARRRRGSAV"
BASE COUNT          275 a          634 c          565 g          265 t
ORIGIN      
        1 ggacaactcg cggtgcgggt tgtccgacgc cccgcccgtg ttcggccttg gccgcgcgta
       61 ccggctgtgg actgtgcccg cgccgcggcc cgtcgacgaa ggcggccgca gcagtactac
      121 cgggtcaccg gccgtggctg agcgagcgtt gcggcgagcc gcaggggggt cgcgtttttc
      181 ggcgagcagt cctcaggcgg ctgagctggt ccaggtgctg gacacctacc ggccggcggt
      241 gacggtggtg cgtgccctgg cacggcgtgg ccggcgcgcc gtggtgggcc gagagcccga
      301 tcgcaggcac cgagcggaat cggcaggcgc tgagtccagc cgccacacca ccgcggtatg
      361 ggatcacccg tcgatcaagg atgaacccgg cgctttcctt gacgcactcg tccacgagct
      421 ggccagacgg ccagagccgg tgcacatcct gccgctcaac gagacagccc tcatgctcct
      481 tgcgcgcgag cgcacgcggc tgcccgcgaa cacatctctg gccgtgccgg ccaccgagac
      541 ggtcgtcacc tgcctggaca aacagcgctg cctgaacgcc gcccaggcag ccggcctgtc
      601 ggtgccccgg tgggaggtcg tcggcggata ccaggagctg cttgccgccg ccggccgagc
      661 ggccgcgccc gtggtcgtca aacccctgtg gccgcattcg agcctgctca acgaccgcaa
      721 ggcgatcctc tgccgtgacg gcagcgatct ggcccgccag atcccggtct ggcccaccgg
      781 ccacgaccgg cttctggtcc agcgctgggc gccaggtttg cgccatgacc tcttgttcgc
      841 ggcccgagac ggcgagctgc tcgacatggt ggaaaagctg accgtccgga cgcaacgccg
      901 cgacgggacg ggcctcgcgg tcgccggcca tacccgcccg gtcagcatcg gactccgcga
      961 ccaactggcc gccctggtgg cccacctgcg ttataccggt gttggcttcg ggcagttcct
     1021 ggtccagccc ggcgcgcagg acggcgcctt cctcgaactc aatccccgcc tcagtgggag
     1081 ccacgtgatc gccgaccacg ttggcctcga tctggccaat ctcgccgtgg acctggcagc
     1141 cgacgtgcca gtcacagctc accttccagc gccgacggga cgcgacggcc acacctatgc
     1201 ctggacgttc ggcgatctca ccgggctctg gtacgcgatc ggtcacagcg agatcacgcg
     1261 cgccggcgcg ctggccgagc tcggccggat cgcccgggcc gcggtgctcg ccgacgtgca
     1321 cgtcacctgg tcctggcgcg atccactacc gacgatcgcc acgtgctggc gataccttgt
     1381 tttacacagc ggaaccgcgg cggcggcgag cacctcgaag cgcaccgcga ttgacggctg
     1441 ataacaatac ttcctcgaac ttctcgaaca gcacgtctct attgaactgc ccgcgtgcca
     1501 gcaccccagc ggccgaccgc gcccgagcca agaaatcaga gtccgtaatg tgttgcgcaa
     1561 tcatcttcgc tccagccaca ggatcgtccg gaggaaccgc gatcccggcc ccggattcac
     1621 ggagcacctc cgccaaccag ccgccgttgt tgatcaccac tggccggctc gcagcaaagg
     1681 cgtcgaacac cttgttggga gagttcgccg acagcgccgg aacgtcagcg aataccgat
//
LOCUS       DATADN010000952         1969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_719562, whole genome shotgun sequence.
ACCESSION   DATADN010000952 DATADN010000000
VERSION     DATADN010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719562"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VHH53_12330"
     CDS             complement(<1..742)
                     /locus_tag="VHH53_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290949.1"
                     /translation="MGTLISVVILVLLIGGGVAYYLQHRQVSQQRELEDSKAEARRWV
                     ERLGGQVLNLVGTDEPSKQALADAAERYTAAGSQMESAQTAAQCRLVTQTALEGLYYV
                     RAARSAMGMDPGPELPSVPGQAAAGSVTEDRAVEVEGHDYAASPHPSDRTPHYYPGGR
                     VAGRPVPQGWYSEPWWKPALVGGAWGLGSVLLFSAMFSGMSGIPAAGAWDQGYDAGQA
                     DAMSDGGGGDFGADGGDAGGDFGGGWGGG"
     gene            884..1687
                     /locus_tag="VHH53_12335"
     CDS             884..1687
                     /locus_tag="VHH53_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2290950.1"
                     /translation="MPEGDTVYLAAKRMTAALSGKRLIRGELRHPRLAEHDLSGLTVS
                     GVHSVGKHMFTRFDDGRSLHTHFRMDGSWHLYRPGQRWRRPGHQARAVLTTQERVAVG
                     FLLHDMALVATQDEHRLVGHLGPDLLKLDWDGHDAATAAQRLREHPGVELGLALLDQR
                     IMAGIGNLYKAEMCFLLGVSPWIPVRDIDPDIVVALARRLLLTNADRPEQSTTGELAR
                     GKQHWVYERSGKPCYRCGEPVIRAVQGNDLHVRSTYYCPRCQPGGHPPP"
     gene            complement(1710..1862)
                     /locus_tag="VHH53_12340"
     CDS             complement(1710..1862)
                     /locus_tag="VHH53_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290951.1"
                     /translation="MAEPEHIQFDLPQLDRRDDPRGGAVAWALLVGGLALVTGIVLAV
                     IWAVNL"
BASE COUNT          356 a          680 c          598 g          335 t
ORIGIN      
        1 cgccgccacc ccagccgcca ccgaagtccc cgccggcgtc gccgccatca gcaccgaagt
       61 cgccgccacc gccgtctgac atcgcgtcag cctgcccggc gtcatagccc tgatcccatg
      121 cccccgcggc agggatcccg ctcattccgg agaacattgc actgaacagc agtaccgagc
      181 ccagacccca tgcgccgcct accagcgccg gtttccacca gggttcgctg taccagccct
      241 gcggcaccgg ccggccggcc acccgaccgc ccgggtagta atgcggcgtg cggtcactgg
      301 gatgcggtga ggctgcgtag tcgtgaccct cgacctccac ggcgcggtcc tcggtgaccg
      361 aaccggcggc ggcctggccc ggtaccgacg gcaattcagg ccccgggtcc atgcccatgg
      421 ccgagcgggc agctcgtacg tagtagagcc cttccaaggc cgtctgggtc accaggcggc
      481 actgtgccgc ggtctgcgcg gactccatct gggagccggc ggccgtgtag cgctccgcgg
      541 cgtcggcaag cgcctgcttg gagggctcat cggtaccgac cagattgagg acctgcccac
      601 cgaggcgttc cacccagcgg cgcgcctcag ccttcgaatc ctcgagctcg cgttgctggg
      661 agacctgccg gtgttggagg tagtacgcca caccaccacc gatcagcagc acgagtatca
      721 ccaccgagat cagcgtgccc acgacgacct cctccaccaa ccagaccgga gcaccgacat
      781 tacccccgct gccggtaggc cccgccgtgc tggcatgggg cctaacgtcg ctgagacagg
      841 gttccgatgt acgcgcttcc attcccttca tctggaggta gccgtgcctg aaggcgacac
      901 cgtgtacctg gcggccaagc gtatgacggc cgcgctgagc ggcaagcgct tgatccgagg
      961 cgagctgcgt catccacggc tggctgaaca tgatctctcg gggctcactg tctcgggtgt
     1021 acacagcgtc gggaagcaca tgttcacccg tttcgacgac ggacgaagtc tgcatacgca
     1081 tttccggatg gacggctctt ggcaccttta ccgacccggg cagcgttggc gacggccagg
     1141 ccatcaagcc cgggcagtgc tgaccacaca agaacgagta gcagtaggtt tcctcctgca
     1201 cgacatggcg cttgtggcca ctcaggacga acaccggctc gtggggcacc tgggccctga
     1261 tctgctcaag cttgactggg acggccacga cgcggcaacg gcggctcaac gactgcgcga
     1321 acaccccggc gtcgaacttg gccttgcgct gctcgaccaa cggatcatgg ccgggatcgg
     1381 gaacctgtac aaagcggaga tgtgcttcct gttgggggta tcaccgtgga tccctgtccg
     1441 cgatatcgat cccgacatcg tggtggcctt agctcggcgg cttctgttga cgaacgccga
     1501 ccgtcccgag cagtccacta ccggggaatt ggcccgcggt aagcagcact gggtgtacga
     1561 gcggagtgga aaaccgtgct atcgctgcgg agaaccagtg atccgcgctg tacaaggaaa
     1621 tgatctccat gtcaggagca cctactactg ccctcgatgt cagcccgggg gacacccgcc
     1681 accctaaggc agcatggcgg cgtggatggt caaaggttca ctgcccagat cactgccagg
     1741 acgatgcccg tgaccagagc tagacctccc acaagcagcg cccatgcaac agccccaccc
     1801 cgcggatcgt ccctgcggtc caactgcggc agatcgaact ggatgtgctc tggctcggcc
     1861 atcactgctc ctcgtctgct ccacgtggtc tggcaggcgc tgagtcacaa aagttacctt
     1921 tgcgacacgt tgtacggatt agcgtttgct tagagtttcc atacttgcc
//
LOCUS       DATADN010000953         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_426771, whole genome shotgun sequence.
ACCESSION   DATADN010000953 DATADN010000000
VERSION     DATADN010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426771"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1653
                     /locus_tag="VHH53_12345"
     CDS             307..1653
                     /locus_tag="VHH53_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290952.1"
                     /translation="MTSTPANTSAPLPDSAAELLADLRQQQRTIAEADARKMLLAAHW
                     AAMSSSDSIVDPADHWHQQLLPIGGEGCPVVAEFCLADLAVCLEMSHDAARAYLSKVV
                     ETRYRLKQCWGRVVAGDLPAWRAMRIAERTITLSPDAATFVDRHVAPVAHKVPLGQLD
                     RLVEEARVRFNPEEALQRARDAAETRHFDVLLDQVTFDGTVRVDGDLDLADAIALNDA
                     VARDAHQQAVLGSTESLDVRRAVAVGNLARNQLALDLDADTADPDARPKGVRRPQQLV
                     LHVHLSEAALDGSGAAPIARVENTRSMVAIEQVAAWAGSDATKVVIKPVIDLNDNIWV
                     GSYEVPDRIDQQVKLRDGTCVHPYCTKRGEICDNDHTHPYDTADPPSGGQTETKNLAK
                     LCRRHHRLKTTSGWTYTSPEPGVYIWTSPYGMRLRRDGGGTEILADLPAPPDPPES"
BASE COUNT          289 a          672 c          573 g          251 t
ORIGIN      
        1 gcctgttggg aggcgccggg ggtccccctg gccgtgggtg cttgcggtga atggctggct
       61 acgagagagg tacgccgtgc ctagccgctg cttctcgggg tgcgggcatc cggtggtcga
      121 gcagggatct ccggtggtcg agcagggagg cgccccggcg ccgaccgttg ccgagacccc
      181 cgcagggaag cagggcggtg acccgtcgca tctcccggga atcccagcaa aataaggcga
      241 aactggcctg tggaagaccc actctcgagg gtcggactgt cggtgtccgg tgcttgagta
      301 ggtgtcatga ccagcacccc cgcgaacacc tcggcgccgc tgcccgacag cgccgccgag
      361 ctgctcgcgg acctgcgcca gcaacaacgc accatcgccg aggccgatgc ccgcaagatg
      421 ctgctggccg cccactgggc ggcgatgagc tcctccgact ccatcgtcga cccggccgac
      481 cactggcacc aacagctcct ccccatcggg ggcgagggct gcccggtggt ggcggagttc
      541 tgcctggccg acctcgcggt gtgtctggag atgtcccacg acgcagcgcg tgcctacctg
      601 tccaaggtgg ttgagacccg ctaccggctc aagcagtgct ggggccgcgt ggtcgcgggt
      661 gacctgcccg cctggcgggc catgcggatc gccgagcgca ccatcaccct gtccccggac
      721 gccgcgacct tcgtcgaccg ccacgtggca ccggtcgccc acaaggtccc gctcggccag
      781 ctggaccggt tggtggagga agcccgggtc cggttcaacc cagaggaagc gttgcagcgc
      841 gctcgagatg ctgctgagac ccggcacttc gatgtcctgc tcgaccaggt caccttcgac
      901 ggcaccgtcc gcgtcgatgg tgatctggac ctggctgacg cgattgcgct gaacgacgcc
      961 gtggctcggg atgctcatca gcaggccgtg ctcgggtcga ccgagtcact cgacgtccgc
     1021 cgtgcggtcg cggtcggcaa cctcgcccgc aaccaactcg ccctcgacct cgacgcagat
     1081 accgccgacc ccgacgcacg gccgaagggc gtacgccggc cgcagcagct ggtgctgcac
     1141 gtccacctct ccgaggccgc cttggatggt tctggcgcgg cacccatcgc ccgggtggag
     1201 aacacccgct ccatggtcgc catcgagcag gtcgcggcct gggccggatc cgacgccacc
     1261 aaggtggtga tcaagccggt catcgacctc aacgacaaca tctgggtcgg ctcctacgag
     1321 gtccccgacc gcatcgacca acaggtcaag ctccgcgacg gcacctgcgt gcacccctac
     1381 tgcaccaaac gcggcgagat ctgcgacaac gaccacaccc acccgtacga caccgcggac
     1441 cctccatccg gcgggcaaac cgagacgaag aacctcgcca agctctgccg gcggcaccac
     1501 cgactcaaga ccaccagcgg ctggacctac acctcacccg aaccaggcgt ctacatctgg
     1561 acctcgccct atgggatgcg gctgcgccgt gacggtggcg gcaccgagat cctcgccgac
     1621 ctccccgccc ctccggatcc acccgagtcg tagcgaccac gccccacccc cgccgaccat
     1681 ccgaccggcg ggggcaccgg catgcccacc caccggttgg tcaccgccca gcccacgttg
     1741 cggggtctcg gcaacggccg gcgccggggc gcctccctgc tcgac
//
LOCUS       DATADN010000954         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_719796, whole genome shotgun sequence.
ACCESSION   DATADN010000954 DATADN010000000
VERSION     DATADN010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719796"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1132)
                     /locus_tag="VHH53_12350"
     CDS             complement(137..1132)
                     /locus_tag="VHH53_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2290953.1"
                     /translation="MVGQQQAASRRATGTEPQGPTELPKRSWWAVLKRTVREFGKDNL
                     TDWAAALTYYSVLSIFPAVIVLTAVLGLLGPSATQTLIDNINTMVPGEGRDVLVNAIT
                     ELQQSQGLAGPLAIIGLGGALWAASGYIGAFMRASNAIYEMPEGRPVWKTVPLRVGLT
                     LAVVVLLALSAVGVVASGAVADRAGQMLGIGSTGVLVWDIAKWPVIAALVSLVFALLY
                     WASPNVKHPGFRWLSPGGVLAVVVWVLASAGFAFYVANFGSYNKTYGSLGGVIGFLVW
                     LWISNIAILLGAELDAELTRGQRIEAGQPAEQEPFLPPRDTRAMKAQDTPPTTNS"
     gene            complement(1247..1507)
                     /locus_tag="VHH53_12355"
     CDS             complement(1247..1507)
                     /locus_tag="VHH53_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HEX2290954.1"
                     /translation="MKILYRPLSMLVSVLGGILAGAVFKKVWAVMSGEEEAPEATSPE
                     YSTKEVLVAAVVQGAIFAGVKAAVDRAGAKGFKKITGKELGT"
BASE COUNT          320 a          603 c          451 g          282 t
ORIGIN      
        1 cgggtgattt gttcggtcgc gtcactgacc aactgcccga ccggacggtc ctcatgttgc
       61 gccggtggtc caccagcatc ctcggacatg atcatccttc cattgcttcc gcccacgtac
      121 ctcacagccg tgctgatcaa ctgtttgtgg tgggcggggt gtcctgcgct ttcatggcgc
      181 gggtgtcacg gggcggcagg aacggttcct gttctgctgg ctggccggct tcgatgcgtt
      241 ggccccgggt cagttcggcg tcgagttcgg cgccgagcag gatggcgatg ttggagatcc
      301 acagccacac caaaaagccg atcactccgc ccagcgaacc gtaggttttg ttgtaggagc
      361 cgaaattggc cacgtaaaac gcaaacccgg ccgatgccag cacccagacc acgacagcca
      421 gcacaccgcc tgggctcagc caacggaaac cggggtgttt gacgttgggg gatgcccagt
      481 acagcaaggc gaacaccaga ctgaccaacg cggcgatcac aggccacttg gcgatgtccc
      541 acaccagcac cccggtcgag ccgatcccca gcatctgccc cgctcgatca gccacagccc
      601 cgctcgcgac taccccgact gcggacaatg ccagcagcac taccaccgcc agggtcagac
      661 cgacccgtag cggcactgtc ttccacaccg ggcgtccttc gggcatctca tagatggcgt
      721 tggaggctcg catgaacgcc ccgatatatc ccgaggccgc ccacaacgca cccccaagac
      781 cgatgatcgc caacggccca gccagcccct gggactgctg caactcggtg atcgcattga
      841 ccaagacgtc ccggccttcc ccggggacca tggtattgat gttgtcgatc aacgtctgtg
      901 tcgccgaggg ccccagcaaa cccaacaccg cggtcagcac gatcaccgcg ggaaagatcg
      961 acaaaacact gtagtaggtc agcgctgcgg cccaatcggt gagattgtcc ttgccgaatt
     1021 cccgcaccgt gcgcttgagc accgcccacc acgaccgctt gggtaactca gtagggcctt
     1081 gcggctcggt acccgtagca cgtctggaag ccgcttgctg ctgtcccacc attcccaccg
     1141 cctcttgcgc cagcgtcggt cgatcgtgcc cgaaagtacg aggcaaaacg tcggacgcga
     1201 cagcgcggca agaaaactcc ctgcaggtag caacaggcac cgcgtcctac gtcccaagct
     1261 ccttaccggt gatcttcttg aaacccttag cgccggcccg gtccaccgcg gccttcaccc
     1321 cggcaaagat cgcgccctgg accacggcgg caaccagcac ctccttggtg ctgtactccg
     1381 gcgaggtggc ctccggagcc tcctcctcac cggacatcac tgcccacacc ttcttgaaca
     1441 ccgcaccggc gaggatccca ccgagcacac tgacaagcat gctcagcggc ctatacaaaa
     1501 tcttcacgcc tcctcctccc gcttgcgtcg acggaggcgc attagcaccc caacacggcc
     1561 aacgctcgcg cccaccgctg aacccgactc gtcctgccct cacggcgctg gttcccggtg
     1621 ccgggcctta tgcggttcta cccctcaccc atcggc
//
LOCUS       DATADN010000955         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1029934, whole genome shotgun sequence.
ACCESSION   DATADN010000955 DATADN010000000
VERSION     DATADN010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029934"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VHH53_12360"
     CDS             complement(<1..534)
                     /locus_tag="VHH53_12360"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEX2290955.1"
                     /translation="MSALGMSAIRRIVHEVRSVEIGATTRYERHQLVVDRDQAEALFA
                     DPALASVRAHSAAPGEQVRIIAPLDVVEPRTKGSGSGAFPGWIAPVNRKRGGDTHVLR
                     GAAVLAAGYLPRNQEGLIDMSGPAAELSPFGRTNNVVIEFDCADGASWQEAEVALRRG
                     LLRLAVHLADAAVAAAPD"
     gene            681..1640
                     /locus_tag="VHH53_12365"
     CDS             681..1640
                     /locus_tag="VHH53_12365"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2290956.1"
                     /translation="MPSESLLRSSPERETCRYEHPGADPHRPMMAAPYELRAHRALET
                     PVLLLATDSWLDAGFGAQGAIAAVLETIPTEVLATFSADEFIDFRARRPTAQISDGVL
                     AGLNWPEIQLRSGQDGVGKSFAVLVGPEPDIAWHAFVRGVVGLAKELGVRLVVSLGAY
                     AAAVPHTRPVQLTAIATMAELAAQVGVVPGAIEVQAGIQTALQEGFAAADIPAIGIWA
                     RVPHYLAALPYPAASAALVEALATVAGLQLDAGELHAAAASVATQIDEVLATSEEHRA
                     LVRGLESVFDGEATPPPDFSGLPSGDELAAELERFLRDEQEGT"
     gene            1798..1926
                     /locus_tag="VHH53_12370"
     CDS             1798..1926
                     /locus_tag="VHH53_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290957.1"
                     /translation="MVWIALAGVGFTIYGPVWLAIIGIVLVRITDACGAMIRPFRG"
     gene            2092..2613
                     /locus_tag="VHH53_12375"
     CDS             2092..2613
                     /locus_tag="VHH53_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX2290958.1"
                     /translation="MADVIGEARATVQPEARAYQHGEDRPLGGYVLVMAVFGALVAGA
                     AGLAAETGRKVPTLGPFDLVLMALGTHKLSRTLSKDAVTSPLRAPFTRYQDTGGPGEV
                     MEEVRHTSQLRHAIGELITCPLCLDMWIATGFALGMVFAPRFTRLVAFTFAALTGADF
                     LQLGYAAAQQAQE"
     gene            2827..>3035
                     /locus_tag="VHH53_12380"
     CDS             2827..>3035
                     /locus_tag="VHH53_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290959.1"
                     /translation="MADPAPRSAEPAGRSAHTELATSRGRIGVRDGLLDDGIAGVTLV
                     RLVRVLDAELDVQHELSLAGFGSDAG"
BASE COUNT          439 a          997 c         1053 g          546 t
ORIGIN      
        1 gtcaggcgcg gccgcgaccg cggcatccgc aaggtgtacg gccaaccgca gcagcccgcg
       61 gcgcagcgcg acctcagcct cctgccacga ggcaccgtcc gcgcagtcga actcgatcac
      121 gacgttgttc gtccggccga acggggacag ctcggcggct ggaccggaca tgtcgatcag
      181 gccttcctga ttgcgtggca ggtacccggc ggccagcacc gcagccccgc gcagcacgtg
      241 cgtgtcgccg ccgcgcttgc ggttgaccgg cgcgatccag ccgggaaaag ccccgctgcc
      301 cgaccccttc gtgcgcggct ccaccacgtc cagcggcgcg atgatccgaa cctgttctcc
      361 cggtgccgcg ctgtgtgcgc gcaccgacgc cagggcagga tcggcgaaca gcgcctcagc
      421 ctggtcccgg tcgaccacaa gctgatgtcg ctcgtatctg gtggtggcgc cgatctcgac
      481 ggagcggacc tcatgcacga tgcgtcgtat tgcggacatg cccagtgccg acacgacggt
      541 ctcccctcgg tggcttcagg gcatcgtaac cacggattca ccgatcatct ccagtgatgt
      601 gctggacaac cacaccttcg tcgcgagcag ggtgctggct gaggcgctta ccggataact
      661 ttgctggccc ggtgatggtc gtgccgtctg aatctctgtt gcgtagctcg cctgagcggg
      721 agacttgtcg ctatgagcat cccggtgcag atccccaccg tccgatgatg gccgctccct
      781 acgagttacg cgctcaccgc gcactggaga caccggtgct gctgctggcc actgacagct
      841 ggcttgatgc tgggttcggg gcacaaggcg ctatagcggc cgttctcgag acaatcccga
      901 ccgaggtgct ggccaccttc agcgcggacg agttcatcga tttccgggcc cgccgtccca
      961 ccgcccagat ctctgacggt gtcctcgccg gcttgaactg gcctgagatc cagctgcggt
     1021 caggccagga cggtgtgggt aagagcttcg cggtattggt cgggcccgag ccggacatag
     1081 cgtggcacgc gttcgtacgg ggtgtcgtgg gcctggccaa ggagctggga gtgcggctcg
     1141 tggtcagcct tggtgcctac gccgccgcgg tgccacacac ccggccggtc cagctgacag
     1201 cgatcgccac catggccgag ctggcggcgc aggtgggtgt ggtgcctggc gccatcgagg
     1261 tgcaggctgg gattcagacc gcgctgcagg agggcttcgc cgccgcggac attcccgcca
     1321 tcgggatctg ggcgcgggta ccgcactact tggcggcatt gccctatccg gcggccagcg
     1381 cggcactggt cgaggcgctg gcgaccgtgg ccgggctgca gctcgacgcg ggtgagttgc
     1441 atgccgcagc tgccagcgtg gcgacccaga tcgatgaggt cctcgccacc agtgaggagc
     1501 atcgcgcgtt ggtgcgcggt ctggagtccg tgtttgacgg cgaagcgacc ccgccacctg
     1561 acttttcggg cctgccgtca ggtgacgagc tcgccgctga gctcgagcgc tttcttcgcg
     1621 atgagcagga gggaacgtag gcgctacgaa ctttccgtgt tccgcggcgg tttaacggcg
     1681 cagggcccgg gtaatgggct gcttaccttt aaccgaaggc gactgggggg cgcctctcac
     1741 cctcggaggg cctcatgctc ggactcccgc tactcgccct ggtgctgtgg ttcgctcttg
     1801 gtttggatcg ctctcgccgg tgtcggcttc acgatctacg gtccggtgtg gctggccatc
     1861 atcggcatcg tgctggtccg catcaccgac gcgtgcggcg cgatgatccg gcctttccgg
     1921 ggttgagatc tcgtacttcc aggcatgtgc gcgtggcggt gatgcagtag gtgtgctcgc
     1981 ccgacggggg ctgctacgcg tgaacgattg acctgtcaca ccgacgaggg gcgtgaccaa
     2041 tccggtcagt gggtatcgcg cagcggagga acattacgag gaagggacga catggccgat
     2101 gtgatcggcg aggcgcgtgc aacggtccag cccgaggcgc gggcgtacca gcacggtgag
     2161 gatcgcccct tgggtggtta tgtcctggtg atggcggtgt tcggcgcgct ggtagcaggg
     2221 gccgccgggc tggccgcgga aaccggccgc aaggtgccta cgctgggccc gttcgacctt
     2281 gtgctgatgg cgttgggaac gcataagttg tcccggaccc tcagcaagga cgccgtcacc
     2341 agccccctgc gggcaccgtt cacccgttac caggacaccg gtggccccgg tgaggtgatg
     2401 gaggaggtgc gccacaccag ccagctcagg cacgctatcg gcgagctgat cacctgtccg
     2461 ctgtgtcttg acatgtggat cgccaccggt ttcgcgctcg gcatggtatt cgcgccccgg
     2521 ttcacccggc tggttgcgtt caccttcgcc gccttgaccg gggccgactt cttgcagttg
     2581 ggctacgccg cggcgcagca ggcacaggaa tgagtagctc ttcggcgccg gctcggccga
     2641 ccgggctacg gtcgtcccgt ggctgccgct agtggccgcg gccaaagccg ggatggacgc
     2701 agccagcggc atcttccttt tcctcgaaca gatcaccgga caccgcaagg tgtgcagctg
     2761 gtgcacgctc gcggcgctgg ccaacgtcgc cacggttcct ctgttgtggc cagaggccag
     2821 gcgtgcgtgg cggaccctgc gccgcgctcg gctgaaccgg ccggtcgcag cgcgcatacc
     2881 gaactggcca cttcccgcgg acgcatcggg gtccgcgacg gcttgctcga cgacggcatc
     2941 gcaggcgtga cgctggtccg gctggtgcgg gtcctcgatg ccgagctcga cgtccagcac
     3001 gagctgtccc tggccgggtt cgggtctgac gccgg
//
LOCUS       DATADN010000956         3308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_427475, whole genome shotgun sequence.
ACCESSION   DATADN010000956 DATADN010000000
VERSION     DATADN010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427475"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VHH53_12385"
     CDS             complement(<1..1559)
                     /locus_tag="VHH53_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX2290960.1"
                     /translation="MGRADGRHDVAPTDQAWWRNAVFYQVYIRSFADSSGDGVGDLDG
                     VRSRLGYLELLGVDALWITPFFRSPMVDHGYDVSDPRDVDPLFGDLDAFDRLVTDAHQ
                     HGLRVTIDLVPNHTSDQHEWFKAAVAAEPGSPERARYIFRDGSSFGGSRPPNNWTSTF
                     GGPAWTQLPPANNAVGQWYLHLFAPEQPDLNWENPEVIADFEQTLRFWLDRGVDGFRI
                     DVAHGMAKPAGLPDMPQPIGACPGLLDTDIADPRFDADGVHDIHRMIRKVLDEYQARV
                     AIGEIWVADDTAFARYIRPDELHMGFNFRLLQADFDADSLREAIEHSLLAVQNSPAPA
                     TWTISNHDTVRPVTRYGGGEVGLRRARAMALVQLALPGPAYLYNGDELGLPNVDLPDW
                     ALQDPVWQRSGRTERGRDGCRVPLPWEGDAPPYGFTSTPGAWLPMPSGWADRTVAAQL
                     EDPASTLSLYRQALQLRREHQTFAGTELLWYGAPPGCFAFLRKEGGLVCALNASEVYV
                     PLPNGQLLLASG"
     gene            complement(1568..1951)
                     /locus_tag="VHH53_12390"
     CDS             complement(1568..1951)
                     /locus_tag="VHH53_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEX2290961.1"
                     /translation="MAAPENFYEAVGGEQTFRQIVGRFYELVKKDEVLRPLYPDEDLG
                     PAEEHLRLFLMQYWGGPHTYSAQRGHPRLRMRHVPFRIGPAERDAWLRCMRTAVDEAD
                     LDEAHRAVLWDYLQMAANSLQNSLT"
     gene            complement(1992..2972)
                     /locus_tag="VHH53_12395"
     CDS             complement(1992..2972)
                     /locus_tag="VHH53_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2290962.1"
                     /translation="MTAELIFQPDCVDEVGSWCSRIFELTHNDMLARYADAVVGTGLR
                     ILLIVTVAVLVRLMAHRAINRLTRVTAEGPQAGLLRPLRERAPESWRGTGLLSTRRSQ
                     RARTIGSVLRSGTTFVVSGVAITLVLGELGINLGPIIASAGILGVALGFGAQNLVKDF
                     LSGMFMMVEDQYGVGDIIDLGEATGTVEAVGLRVTTLRDVNGTVWYVRNGEVLRVGNS
                     SQGYSVAVVDLPVGHDADIPETSELAGRTAIELTAEGSALADDVLEPPEVLGVESISP
                     GGVTLRITTKVRAGRQWAVQRALLAGIKDAFADAGIPPPSPVSGLAAPPT"
     gene            complement(2969..>3308)
                     /locus_tag="VHH53_12400"
     CDS             complement(2969..>3308)
                     /locus_tag="VHH53_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX2290963.1"
                     /translation="DEYGTADDPAELDWLRSYSPYHQVRPGTSYPAVLFTVFDSDSRV
                     DPLHARKMCAALQHATTADPARAPVLIRREIEVGHAVRAVSRTVALATDQLCFLAQYT
                     GLDLEAVHTG"
BASE COUNT          564 a         1129 c         1104 g          511 t
ORIGIN      
        1 ccgctggcca gcaacagctg cccattcggt agcggaacat atacctcgga ggcgttcaga
       61 gcacagacca gcccgccctc cttgcgcagg aaggcgaaac atccaggggg tgcgccgtac
      121 cacagcagct cggtgccggc gaacgtctga tgctctcggc gcagttgcag agcctgccgg
      181 tacagcgaca gcgttgaggc ggggtcttcc aactgggccg ccacggtacg gtcggcccag
      241 ccgctgggca tcggcaacca ggcgccgggg gtggaggtga acccgtaagg cggagcatcg
      301 ccctcccacg gcagcggcac ccggcagccg tcgcgcccgc gctcggtgcg cccagagcgc
      361 tgccacaccg ggtcctgtag cgcccagtca ggcaggtcga cgttcggcag gcccagctcg
      421 tcgccgttgt acaggtacgc cggcccgggt agggcaagtt ggaccaacgc catcgcacgt
      481 gcgcgccgca ggcccacctc gccaccgccg tagcgggtca ccggccggac cgtgtcgtgg
      541 ttggagatcg tccaggtagc cggtgccggg ctgttctgga cagccagcag ggaatgctcg
      601 atggcctcgc gcaggctatc ggcgtcgaaa tccgcctgca gcagccggaa gttgaatccc
      661 atgtgcagct cgtcggggcg gatgtaacgg gcgaaagcag tgtcgtcggc cacccagatc
      721 tcgccgatcg ccacccgcgc ctggtactcg tcgagcacct tgcggatcat ccggtggatg
      781 tcgtgcacac cgtcggcgtc aaagcgggga tcggcgatgt cggtatcgag caagcccggg
      841 caggccccga tgggctgcgg catgtccggc agcccagcag gcttggccat cccgtgcgcc
      901 acgtcgatgc ggaacccgtc cactccccgg tccagccaga accgcagagt ctgctcgaaa
      961 tcggcgatca cctccgggtt ctcccaattc aggtcgggct gttcgggcgc gaacaggtgc
     1021 aggtaccact gcccgactgc gttgttcgcc gggggcagct gggtccaagc cgggccgccg
     1081 aaggtactgg tccagttgtt cggcggccgc gacccaccaa agctggagcc gtcccggaag
     1141 atgtaacggg ctcgctcggg gctccccggt tcggcagcca cggccgcctt gaaccactcg
     1201 tgctggtcgg aagtgtggtt gggcaccagg tcgatggtca cccgcagccc gtgctggtgg
     1261 gcgtcggtga ccagccgatc gaaggcgtcc agatcgccga acagcgggtc gacgtcacgc
     1321 ggatcgctga cgtcatagcc gtggtcgacc atcggggaac ggaagaatgg agtgatccac
     1381 agcgcatcca cgccgagcag ctccaggtag cccagccggc tgcgcacacc gtccagatca
     1441 cccaccccgt caccgctgga gtcggcgaag gagcggatgt aaacctgata gaacaccgca
     1501 ttgcgccacc aggcctggtc tgtgggagcc acgtcgtggc ggccgtcggc tcgtcccatg
     1561 tgttccctca agtcagcgag ttctgcaggc tgttcgctgc catctgtaag tagtcccata
     1621 gcacggcccg gtgggcctcg tcgaggtcag cctcgtcgac cgcggtgcgc atgcagcgca
     1681 gccaggcgtc acgttcggcc ggcccaatcc ggaacgggac gtgccgcatc cgcagccgcg
     1741 ggtgccctcg ctgcgcggag taggtgtgtg gcccacccca gtactgcatg aggaacagtc
     1801 gcaggtgctc ctcggcggga ccgaggtcct cgtccgggta gagcggacgc agcacctcgt
     1861 cctttttcac cagctcgtag aagcggccga cgatctggcg aaacgtctgt tcgccgccga
     1921 cggcctcgta gaagttctcc ggagcagcca cccccccatc ttgccttgtg gaccaagcgc
     1981 tgcggaaaac ctcaggtcgg tggcgcggca aggccgctga caggtgacgg cggcggaatc
     2041 cccgcatcgg cgaaagcgtc cttgataccg gccagtagcg cccgctgcac cgcccactgc
     2101 cggccggctc ggaccttggt ggtaattcgc agtgtcacgc caccgggact gatcgactcg
     2161 actccgagca cctccggcgg ttcgagcaca tcgtcggcca gtgcgctgcc ctcggcggtg
     2221 agctcgatgg cggtgcgacc ggcgagctcg ctggtttcgg ggatatccgc gtcatggccg
     2281 acgggaaggt cgacgacggc caccgagtag ccctgactgg agttgccgac ccgcagtacc
     2341 tcaccgttgc ggacgtacca cacggtgccg ttgacgtcgc gcaaggtggt cacccgcaga
     2401 cctacggcct ccaccgtgcc ggtggcttcg ccgaggtcga tgatgtcgcc gacgccgtac
     2461 tggtcctcga ccatcatgaa catcccggac aggaaatcct tgaccaggtt ctgcgccccg
     2521 aagccgagcg cgacgcccag gatgccggcc gaggcgatga tcggcccgag attgattccg
     2581 agctcaccca gtaccagggt gatcgcgacc ccggagacca cgaacgtcgt gccggaccgc
     2641 agcactgaac cgatcgtgcg ggctcgttgg gaacggcgtg tggacagtag ccccgttccg
     2701 cgccaggact cgggggcccg ctcccgcagc gggcgaagca acccagcctg cggtccctcc
     2761 gcagtcaccc gggtcaaccg gttgatcgca cggtgcgcca tcagccgaac cagcaccgcg
     2821 accgtgacga tgagaagaat ccgcagcccc gttcccacca cggcgtcggc gtagcgggcc
     2881 agcatgtcgt tgtgggtgag ctcgaagatg cgcgagcacc acgagcccac ctcgtcgaca
     2941 cagtctggct ggaaaatcaa ctccgcggtc atccggtgtg caccgcctcc agatcgagcc
     3001 cggtgtactg ggcgagaaag cacaactggt cggtggccag cgccacggtc cgggataccg
     3061 cgcgcaccgc atgccccacc tcgatctcgc ggcgaatcaa cactggagcc cgcgccgggt
     3121 cggccgttgt ggcgtgctgc agggcagcgc acatctttct ggcatgaagt ggatcgaccc
     3181 ggctgtcgga gtcgaacacc gtgaacagca ccgccgggta gctggtgccc ggccgcacct
     3241 ggtgatacgg ggagtacgac cgcagccagt ccagctcggc gggatcgtcg gcggtgccgt
     3301 actcgtcg
//
LOCUS       DATADN010000957         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_102111, whole genome shotgun sequence.
ACCESSION   DATADN010000957 DATADN010000000
VERSION     DATADN010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102111"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VHH53_12405"
     CDS             complement(<1..240)
                     /locus_tag="VHH53_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX2290964.1"
                     /translation="MLPATTETGTSPDFVVAVFTAVSAVCVTGLIVVDTPTYWSTFGE
                     LVILGLVQAGGIGIMTLATLLGLLIARRMGLRLQLT"
     gene            435..1115
                     /locus_tag="VHH53_12410"
     CDS             435..1115
                     /locus_tag="VHH53_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2290965.1"
                     /translation="MSRPSPQNLPSATRARRRLSSQARRGELLDAALREFSSRGYYLT
                     QMEHVAATAGVSKALVYQHFPSKEELFAAVTEQVVEGFMGRLPEVLGKAGDALGAWRG
                     VVRLLCDLVSERPEAWTLVARHLDNPEVGASLRGLREQMNEVLAAVLASYYAPEQGGV
                     VPSEQEVMEQARLTVPLLVGALQGLMSWWLEHPEVPRAKVEARAVAFGWLGLDRIRRG
                     EWLPLASD"
     gene            1162..2079
                     /locus_tag="VHH53_12415"
     CDS             1162..2079
                     /locus_tag="VHH53_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HEX2290966.1"
                     /translation="MSMTSTVDARTSRIPDREETAQRLLRTSAKHSYDPAVEIDWDAP
                     LAPDRYFIDPRRSSLYGTAIWDRMSEQQRIELTKHELASLYCMGIWFETILMQMLVRH
                     VYDRPKGSAHARYAWTEIADECRHSVMFSRAAEKFGGPDYGPGWLVQHLGRLLKTTSN
                     GTLTFSGAIYVEEILDAVQREQMADESLQPMVQKVSWIHVVEEARHIRYARDETRRQW
                     PRLGWLGRAYSRLVLALVVYFSTTRLIDPKVYATVGLDVDEAVAAAAANPHWRQTKQF
                     AARKVIDFFNEVGLIAGPGKLLWRRAGVL"
BASE COUNT          365 a          699 c          751 g          316 t
ORIGIN      
        1 ggtcagctgg agccgtaacc ccatgcgccg ggcgatgagc aggccgagca gcgtcgccaa
       61 ggtcatgatc ccgatgccac cggcctgcac caacccaaga atcaccagtt caccaaaagt
      121 ggaccagtac gtcggcgtgt ccaccacgat caggccggtg acgcacacgg ccgagacggc
      181 ggtgaacacg gccacgacga aatcaggcga cgtgccggtc tcggtggtgg cgggcagcat
      241 cagcaacgcc gtgccgatcg ccgcggcgac ggcgaatcca gcgacgaccg cggcggccgg
      301 ccgacggagt aacccggcaa ccggcgacca ctggcgaagc accgcgatca ccacgaaacg
      361 aagactagct agcctgctcc gccgcttggg gagtgcgcgc tcacggcgca tgggcagcga
      421 gcacactggt tggcgtgagc cggccgtccc cgcagaacct tccgagcgcc acccgcgccc
      481 ggcgccggct gtcgtcccag gccagacgag gtgagctgct cgatgcggcg ctgcgggagt
      541 tcagcagccg gggttactac ctcacccaga tggaacacgt cgccgccacc gccggggtga
      601 gcaaggcgct ggtgtaccag cacttcccga gcaaggagga gctcttcgca gcggtgaccg
      661 agcaggtcgt ggagggtttc atgggaaggc tgcccgaggt gttgggcaag gccggtgacg
      721 cgctgggtgc ctggcgcggt gtggtccggc tgctctgcga cctggtgagt gagcgtccgg
      781 aggcgtggac attggtcgcg cggcacctgg ataacccgga ggtcggcgcg tcgctgcgcg
      841 ggctccggga gcagatgaat gaggtgctgg cggcggtact cgccagctac tacgcccccg
      901 agcagggggg tgtggtgccc tccgagcagg aggtaatgga gcaggcacgg ttgaccgtcc
      961 cgttgctcgt cggcgcgttg cagggcctga tgtcctggtg gctggagcac ccggaggtgc
     1021 cgcgcgcgaa ggtggaggcg cgggcggttg ctttcgggtg gctcggcctg gaccggatcc
     1081 gccggggtga gtggcttccg cttgccagtg actgacgagt cagttacttt tgagggaacc
     1141 cacccgtcaa ggagaccgca catgtccatg acgagcaccg ttgacgcgag gaccagccgc
     1201 attccggacc gggaggaaac cgcgcaacgg ctgctgcgga cgtcggccaa gcattcctac
     1261 gacccggccg tcgagatcga ctgggacgcc ccgctcgcac cggaccgcta cttcatcgac
     1321 ccgcgccgca gctcgctgta cggcaccgcg atctgggacc ggatgagcga gcagcagcgg
     1381 atcgaactga ccaagcacga gctcgcgagc ctttactgca tgggcatctg gttcgagacg
     1441 atcctcatgc agatgctcgt ccggcacgtc tacgaccgcc ccaagggcag cgcgcacgcc
     1501 cggtacgcct ggaccgagat cgccgacgag tgccggcact cggtcatgtt ctcccgcgcc
     1561 gcggagaagt tcggcggccc ggactacggt cccggctggc tggtgcagca cctcgggcgg
     1621 ctgctcaaga cgacctccaa cggcaccctg accttcagcg gcgcgatcta cgtcgaggag
     1681 atcctcgacg cggtgcagcg cgagcagatg gcggatgagt cgctccagcc gatggtgcag
     1741 aaggtgtcgt ggattcacgt ggtcgaggaa gcccgccaca ttcgctacgc ccgcgacgaa
     1801 acccgtcgcc aatggcctcg gttgggctgg ctgggccgcg cctacagccg cttggtcctc
     1861 gcgttggtcg tctacttctc caccaccagg ctgatcgacc cgaaggtgta cgcaacggtc
     1921 gggctggacg tcgacgaggc cgtcgcggcg gctgcggcca acccgcactg gcggcagacc
     1981 aagcagttcg ccgcccgcaa ggtcatcgac ttcttcaacg aggtcggcct catcgccggg
     2041 ccgggaaagc tgctctggcg ccgggccgga gtgctgtagc gccgccaacg cgttcagcag
     2101 gctgggcggg gttattgcgg tgccgataac c
//
LOCUS       DATADN010000958         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_574090, whole genome shotgun sequence.
ACCESSION   DATADN010000958 DATADN010000000
VERSION     DATADN010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574090"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VHH53_12420"
     CDS             <1..980
                     /locus_tag="VHH53_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HEX2290967.1"
                     /translation="VDPRGTQHCETSALGVLLRHQGLDLSEPMLFGLGSGLSFIYWDS
                     KNMDFPFLGGRVKPFELTRNLAARLGLELAVQETGSARKAWENVATAVDAGRPVGLQL
                     DSYHLDYFTSKVHFGGHVVALYGYDEHDAYLVDTDQQGGAVTTSLSSLARARAARGPM
                     TARHRSFTLTAPRSPPAPPPRIVPAITTCADAFLNPPIANLGHRGVEKAGKLIRTWLQ
                     RTDDPQRDLPRAALLMEKAGTGGALFRNLYRDFLAECTQLLDSSHLRTGHRLYTEAAT
                     LWTEVAALITKAGESGDAQCLAEAGAVLCDLSRLERDAMQALSCLRQGA"
     gene            complement(1197..1349)
                     /locus_tag="VHH53_12425"
     CDS             complement(1197..1349)
                     /locus_tag="VHH53_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290968.1"
                     /translation="MSGSVAIVLAARHPHLVSRMVLIDAHLDPIPRAPGSSGSSGIAA
                     YSEESS"
     gene            1348..>1529
                     /locus_tag="VHH53_12430"
     CDS             1348..>1529
                     /locus_tag="VHH53_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290969.1"
                     /translation="MLWAISSAAETPAAVRELASVRVCFQRVVEVRWAVADAVAQEVQ
                     QRRAAAGQPRVDRDFR"
BASE COUNT          254 a          549 c          509 g          217 t
ORIGIN      
        1 gcgtcgaccc tcgcggtacg cagcactgcg agacgtcggc tctgggagtg ctgctgcggc
       61 accaggggct cgatctgtcc gagcccatgc tcttcggcct cgggtccggg ctgtccttca
      121 tctactggga cagcaagaac atggactttc ccttcctcgg cgggcgggtc aagcccttcg
      181 aactcaccag gaacctggcc gccaggctgg ggctggagct cgcggtccag gagaccggct
      241 ccgcccgcaa ggcgtgggag aacgtggcga ccgccgtcga cgccggccgc cccgtcgggc
      301 tccagctcga cagctaccac ctggactact tcacctcgaa ggtgcacttc ggcggtcacg
      361 tcgtcgccct gtacggctac gacgagcacg acgcctacct ggtggacacc gaccagcagg
      421 gcggagcggt gaccacgagc ctgagcagcc tcgcccgggc cagggccgcg cgcggaccga
      481 tgaccgccag gcaccggtcc ttcaccctca ccgccccgag gagcccgccc gctccaccac
      541 cccggatcgt tcccgcgatc accacctgcg ccgacgcctt cctcaaccca cccatcgcca
      601 acctcggcca ccgaggcgtc gaaaaggccg gcaagctgat ccgcacatgg cttcagcgca
      661 ctgacgatcc gcagcgggat ctgccgcggg ccgccctctt gatggagaag gccggcaccg
      721 gcggcgctct gttccgtaac ctctaccgtg acttcctcgc cgaatgcacc caactcctcg
      781 acagcagcca cctgcgcacc ggtcacaggc tgtacaccga ggcagccacc ctgtggacag
      841 aagtcgcagc actgatcaca aaggccggtg aatcaggcga tgcgcagtgc cttgcagagg
      901 caggcgccgt gctctgcgac ctttcacgac tggagcgcga cgccatgcag gcgctgagct
      961 gccttcgcca aggggcttga agcaggcaac tggagcattc gtgccaccgt cgatgcgcaa
     1021 aaccccagca ggccggaagc aacggtgacc caaacgctcc actcgctgac gggtgggggt
     1081 cgtgccgcgc gtgaggtgga acgcgctgcg gtgcagggcc tcccggccgg ccaggcgcat
     1141 ggtggaccac cagtgggagc tgacccggcc gcggacctcc tcccagccgc cgcctgtcac
     1201 gaactctcct cggagtaggc ggcgatgccg ctgctgcccg acgagccggg cgcacgcggg
     1261 atcgggtcca ggtgcgcgtc gatcaggacc atgcgggaga ccaggtgggg gtggcgggcc
     1321 gccaggacga tcgccaccga gccgctcatg ctgtgggcga tcagttcggc ggccgagacg
     1381 cccgccgccg tcagggagtt ggcgagcgtc cgcgtgtgct tccagcgtgt agtcgaagtg
     1441 cgttgggcgg tcgctgatgc cgtggcccag gaggtccagc agaggcgagc agcggccgga
     1501 caacctcggg tcgaccgcga cttccgtga
//
LOCUS       DATADN010000959         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_259027, whole genome shotgun sequence.
ACCESSION   DATADN010000959 DATADN010000000
VERSION     DATADN010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259027"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VHH53_12435"
     CDS             complement(<1..568)
                     /locus_tag="VHH53_12435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX2290970.1"
                     /translation="MHNHVNGLKTALLLGGLSAMVVLIGSFFGRGGVLIAVVLMLAMN
                     AYAYFNSDKIALRAMHARPVSEAEQPMMYRIVRELATDARQPMPRLYVSPTQAPNAFA
                     TGRNPRHAAVCCTVGILELLDERELRGVLGHELSHVYNRDILISSVAGALAGMVSALA
                     NLAMFAGLFGGGGDDRPNPIAMILIALLG"
     gene            complement(684..1532)
                     /locus_tag="VHH53_12440"
     CDS             complement(684..1532)
                     /locus_tag="VHH53_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HEX2290971.1"
                     /translation="MSLPLKLAPLLRVGTGRRYVMCPPRYFTVSYTINPWMDPSQPVD
                     TTRAMAQWTALANTHRALGHTVEVIEPQPGLPDMVFAANAGTVINGVVLGARFRNTQR
                     AAEAEHYRRWFVTHGAPDMVMPSAVNEGQGDLLWTGRLLLAGTGFRTEPAAHLEAQEL
                     LGVPVVSLQLIDPYYYHLDTCLLVLDDSQTTPLIAYYPAAFSPGSRRVLERLFPDAVI
                     ASAADAACLGLNGVSDGCSVVLPLEAVDLGCALAARGFEPVFVDVSELRRAGGGPQCC
                     TLQLRP"
     gene            1606..>1711
                     /locus_tag="VHH53_12445"
     CDS             1606..>1711
                     /locus_tag="VHH53_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HEX2290972.1"
                     /translation="MDALDRQIVTQLVADARSSYSEIGSIVGLSAPAVK"
BASE COUNT          301 a          555 c          580 g          275 t
ORIGIN      
        1 ggccgagcaa cgcgatcaga atcatggcga tcgggttcgg acgatcgtcg ccgccgccgc
       61 cgaacagtcc cgcgaacatc gccaggttcg ccaacgccga gaccatgccg gccagtgctc
      121 cggccacgga ggagatcaag atgtcccggt tgtagacatg cgacagctca tggccgagca
      181 cgccacgtag ctcgcgttca tccagcagtt cgaggatgcc gacggtgcag cacaccgctg
      241 cgtggcgcgg attgcggccg gtggcgaagg cattgggtgc ttgggtcggg ctgacgtaca
      301 gccgtggcat cggctggcgg gcgtcggtag ccagctcccg gactatccgg tacatcatgg
      361 gctgctcggc ctcggacacc ggccgcgcgt gcatcgctcg caacgcgatc ttgtccgagt
      421 tgaagtaggc gtaggcgttc atcgccagca tgagcaccac tgcgatgagt accccgccgc
      481 ggccgaagaa cgatccgatc agcacgacca tcgccgaaag cccgccgagc agcagcgcgg
      541 tcttcagccc gttgacgtgg ttgtgcacgg tggtcgacct ccctggtgta gtcggctcca
      601 atctctgcaa cgcgcccgtt ggctgccggg ttccgcgcac acgtgcacgc gacaccccgg
      661 gcacgggagc ctgacacgcg gggctagggg cggagttgga gggtgcagca ttgggggccg
      721 ccgccggccc tgcggagttc tgagacgtcc acgaagacgg gctcgaagcc acgagcggcc
      781 agcgcgcaac ccagatcgac cgcttcgagg ggtagcacca cggagcagcc gtcggacacc
      841 ccgttgagcc ccaggcacgc cgcgtccgcc gcgctggcga tcaccgcgtc ggggaacagg
      901 cgctccagta cccgtcgtga gccgggtgag aacgccgcag gatagtaggc gatcagtggc
      961 gtggtctgcg aatcgtcgag caccagcagg caggtgtcga gatggtagta gtaggggtcg
     1021 atcagctgaa gcgagaccac cggcaccccg agtagctcct gcgcttccag gtgcgccgcg
     1081 ggttcggtgc gaaacccggt gccggccagc aggagccgac cggtccagag cagatctccc
     1141 tgcccctcgt tgaccgcact gggcatcacc atgtcgggtg cgccgtgcgt gacgaaccag
     1201 cgccggtaat gctccgcctc agcggcgcgc tgggtgttgc ggaaccgggc gcccagcaca
     1261 acgccattga tcacggtgcc ggcgttggcc gcgaacacca tatccggtag gcccggctgg
     1321 ggctcgatca cctccacggt gtgtccgagt gcgcggtgcg tgttggccag cgcggtccac
     1381 tgcgccatcg cccgtgtggt atccaccggc tggctggggt ccatccaagg gttgatcgtg
     1441 tagctcacag tgaaataccg cggcgggcac atcacgtacc ggcgcccggt gcctacccgc
     1501 agtaacggcg ccaacttcag aggcaacgac atgattcgat ggtatggaac ggaatatacg
     1561 ggcttccata tcgttcattg cgcgtatacc agcagaatat tgcgcatgga tgcgcttgac
     1621 cgtcagattg tgacgcagct ggtggccgac gcccgctcca gctactccga gatcggcagc
     1681 atcgtcgggc tttcggcgcc cgctgtgaag c
//
LOCUS       DATADN010000960         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1312063, whole genome shotgun sequence.
ACCESSION   DATADN010000960 DATADN010000000
VERSION     DATADN010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312063"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            908..1288
                     /locus_tag="VHH53_12450"
     CDS             908..1288
                     /locus_tag="VHH53_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290973.1"
                     /translation="MFAVLAPPEFGRLTKSAHDHHADVLAGWPSKRPVMVQAGSPPRC
                     LSVLLHDPAAVALFLRTHMKPDELMELILILADAAADVLRSRRAIYGHYPPRQRQQSA
                     SAKAAVKSTVAYSGTNQAAALAAP"
BASE COUNT          365 a          622 c          591 g          367 t
ORIGIN      
        1 gcgtgcaaag atgcccgtcc tatgcaggat ggcaatcccg acaccgataa ccgcaattcc
       61 aaagcctatg cccgcaaccc cgattagcac tcaccgtcgc gcaggatgac gatcccggga
      121 ccgatgaccg cgatctcgcc attgatccac gcgatcctga gcagcacatt gctattgtga
      181 ggatggcgac tccgccgatc actggatggg aacactttta tcgtgatcta tgaggtcacg
      241 ggtggtgggg gcggccccca gatcaacaac ttcggggtcc gtggtattgg cactatctat
      301 ttgccgaagg aagagtccgt ctcgacgtgc tcgtcggggg tgactttgac ggtcaaggcg
      361 acgagtatct cggagaatta aacacaagtg tggcgcgggg acctgtcgaa ttctttgtta
      421 tacgtcacct tcgcctgaag gacggccacc aatgtctggc cgagcgatga aggtgctgtc
      481 cgcggtaatt atggtggtcg tctacgccgt ggtggttttg gtcatggcct cgatcgttgg
      541 cgtgctgtga aggccgcgag aacttatcga gtgcggccct cgcttcggcc agttaccccg
      601 gttgctggcc ctggtcgcgc atagaaagca cagggagacc gcacaccgga acaccctgcc
      661 gaacagtgga cgcgcgtacg acccgcacgg cgagctgtac cggtctggct gaccagtccc
      721 cctggccggt tactggccgc acgatcatcc ctgtggtggg tctggctgtc acggtgggcg
      781 atggatgtgt aacactgcgc aggcgagggg cgattgcagt gatgcccagg agcatgactc
      841 ccaatccctc ggcaggtgaa ccgtggccat ccagactcgt ccgcccccgg ttggcgctgc
      901 gcccagcttg tttgccgtcc tggctccgcc ggagttcggc aggctgacta agagcgccca
      961 cgatcatcac gccgatgtgc tcgcaggatg gccatccaag cgtccggtga tggtccaggc
     1021 cggttcccca ccgcgctgtc tgtccgttct gctccatgac ccggctgctg tcgcactatt
     1081 tctgcgcacc catatgaaac cggatgagtt gatggagctg atccttatcc tcgccgatgc
     1141 ggcagcggat gtattacgat cccgacgcgc catttacggc cattatccgc ctcgtcagcg
     1201 tcagcagtcg gcctcagcta aagcggccgt taagtcgaca gttgcttact ccgggacgaa
     1261 ccaggcagcg gcgctggccg caccgtgatc gggctgggac tagacgatga ctaaccgctg
     1321 ataaacgccg ctcagtgaac ggccgcggtg cctgcacggc ccggggccgc ctcggtcgcc
     1381 aacccagccc ccacatcgcg ggagcggtcg tcgaccgtgg tttcagagcc agtatcggcg
     1441 atcactacag cccgcgcagc caccgcaggc tgcggaaccg acgagctgac ggagacatca
     1501 ctgatcgtgc ttgagctagt gctccttgtg acagtatggg ttttgggctt gactgcggta
     1561 cacctggtga aaccaccacc ggctccgcgg cttgagcacc cggagccggt ggacgggcaa
     1621 ttcatcgagc atgctcgccc cttgcccctc tgagctagtt gcgcaccagg ccctgctgcc
     1681 gggcactact gcactatcgc gctgctcggg gacagccgaa tacttgacta acgcgcacag
     1741 acatgaccag cctttgctca ccaagggtga cctgaaccgc gggcgagcac ccgagctgaa
     1801 gagcccgcac tggcgctgcg gcatcgtcga ccccgccacc ggcgcaacct gcacgctgcg
     1861 accacacacc aacaacaggc aacaccagag tcgtcagctg tgcagggcgc cgggcgatcc
     1921 acctgcatcg tcacctggac cgggg
//
LOCUS       DATADN010000961         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_428046, whole genome shotgun sequence.
ACCESSION   DATADN010000961 DATADN010000000
VERSION     DATADN010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428046"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..614
                     /locus_tag="VHH53_12455"
     CDS             123..614
                     /locus_tag="VHH53_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX2290974.1"
                     /translation="MNSRDRDPTGRARNARPRDALGRPLPHGAQGVSRVPEDVVLSAG
                     EAVRLAQQLIDDGYPFHAHEVFEGVWKATSGQQRELWQGLAQLAVGLTHMQRGNPKGA
                     AALLRRGAARIDPYAAGSPHGLDIAGLVGHARTLATRVERDGVDAVSSGDLRPRLLPT
                     AFD"
     gene            complement(696..1046)
                     /locus_tag="VHH53_12460"
     CDS             complement(696..1046)
                     /locus_tag="VHH53_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2290975.1"
                     /translation="MSSPDQITTEPIGLDEARRLVTSGAAVLVDVREQHEWDAGHAPE
                     AVHMPIGELDAAALPPGTQIVTTCRSGGRGSRAAAALLEAGLPARNLAGGMRGWRDAG
                     LPLESSNGEAGSVD"
     gene            1203..>1783
                     /locus_tag="VHH53_12465"
     CDS             1203..>1783
                     /locus_tag="VHH53_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2290976.1"
                     /translation="MRIGMPLNYGGGFAETVAELADYEKAGLDIVFVPEAYSFDAVSQ
                     LGFIAARTERLQIASGILQIYTRTPALTAMTAAGLDYVSGGRFTLGIGASGPVVIEGF
                     HGVPYTAPLGRTRELVEICRMVWRRERLEYQGRHYQIPLPAQPGASQRGKPLKLINHP
                     VRERIPIVIAAIGPKNVAMAAEIAEGWQPMFYLP"
BASE COUNT          279 a          594 c          624 g          286 t
ORIGIN      
        1 gtcggtgtgc tcacgacgac cgcggtgatc catgccccca tcctgcctgc atctatcccg
       61 atccggcggg tgctctcgcc cagaaggcgt gccccgggac atcatgggga catcatgggg
      121 aaatgaacag ccgtgaccgg gaccccaccg gccgggcccg caacgccagg ccccgagatg
      181 ccctaggccg gccgctgccg cacggtgcgc agggcgtcag ccgggttccg gaggacgtcg
      241 tgctgagcgc gggggaggct gtgcggctcg ctcagcagct gatcgacgat ggttacccgt
      301 tccacgctca cgaggtgttc gaaggggtat ggaaggcgac ctcggggcag cagcgcgagt
      361 tgtggcaggg cctagcgcag ctcgcggtcg gcctcactca catgcagcgg ggcaatccga
      421 agggcgcggc agcactactg cgccgcggcg ctgctcgaat cgacccatac gctgccggct
      481 ccccgcacgg gctagacatc gcgggtctgg tcggacacgc ccgtacgctc gctacccgcg
      541 tcgagcggga cggtgtggac gcggtgtcgt cgggagacct gcgaccccgg cttctaccga
      601 ccgcgttcga ctagccctcg cacacctgcg cacccggcgc gggacgaaga ttcttacatg
      661 aatgcgcggt gatcggcggg gcaagatccg cagtatcagt ccaccgagcc tgcttcacca
      721 ttggacgact ccaacggcag cccggcatcg cgccagccgc gcatcccgcc ggccagattg
      781 cgtgccggca atcccgcttc gagcaacgct gcggccgccc gcgatccccg cccgcctgag
      841 cggcaggtgg tgacgatctg cgtgccgggc ggtaatgcag cggcgtcgag ctccccgatt
      901 ggcatgtgaa cggcctcggg ggcgtgtccg gcgtcccatt cgtgctgctc tcggacgtcc
      961 accagcacgg ctgcacctga agtgaccaac ctgcgcgcct cgtcgaggcc gatgggctcg
     1021 gtggtgatct ggtcggggga gctcatgggc gaacctcctc ggcatcgtcg ccagcgttgc
     1081 tcaacgttac gtcgccaccc acgtggtcag gctactcaac gttaccgggt gctgtcggct
     1141 gccgcgcctg gcaggctgcc ggtgcagggg cggtagctgc tgtcgcgcga ggaggaaatg
     1201 cggtgcgcat cgggatgccg ttgaactacg gcggcgggtt tgccgagacc gtggcggagc
     1261 tggccgacta cgaaaaggcc gggttggaca tcgtgttcgt acccgaggcc tacagtttcg
     1321 acgcggtcag ccagctgggg ttcattgccg cgcgcaccga gcggctgcag atcgcctcag
     1381 gaatcttgca gatctacacc cgtaccccgg cgctcaccgc gatgaccgcg gccgggctgg
     1441 attacgtgtc gggcggcaga ttcaccctgg gcatcggcgc atcgggcccg gtggtgatcg
     1501 agggtttcca cggtgtgccc tataccgcgc cgctgggccg cactcgggaa ctggtggaga
     1561 tctgccgcat ggtgtggcgc cgggagcggc tggagtatca gggcaggcac taccagatcc
     1621 cgttacctgc ccagcctggg gcaagccagc ggggcaagcc gctgaagctg atcaaccacc
     1681 cggtgcggga acggatcccg attgtcatcg ccgcgatcgg cccgaagaac gtggcgatgg
     1741 ccgcggagat cgccgagggc tggcagccga tgttctacct gcc
//
LOCUS       DATADN010000962         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1480638, whole genome shotgun sequence.
ACCESSION   DATADN010000962 DATADN010000000
VERSION     DATADN010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480638"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VHH53_12470"
     CDS             <1..821
                     /locus_tag="VHH53_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2290977.1"
                     /translation="LFSIFTVFALGFAFYLTFREWNILEPEKPFIGLQNYRDMIGDED
                     FRRSVINTFYFTGASVPLGMAVGLGVALLLNQALWGRTLLRTLFFLPVVTPFVVAAIV
                     WKWLYNGDFGPINYYLLKTHLISEPLLWLSDKNLAMPAVVLMSVWTSVGFSMVVYLAA
                     LQGVPAELYESARIDGAGAWARFRYVTLPMLAPSTLFLMVMGIIGSFQVFTQIYIMTS
                     GGPVNRTSTMVYYIYESAFKFYEMGYASTLAYALFVLLLVFTVIQLRLYRRADL"
     gene            911..>1588
                     /locus_tag="VHH53_12475"
     CDS             911..>1588
                     /locus_tag="VHH53_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2290978.1"
                     /translation="MAVSYAILVPGAILFVAPFAWLVSASFQHVGDIFSWPPQWIPQN
                     PTLDGYKGFFGIGRPGELAQFNEGAWRWFVNSAFVAVSVTVLQLFFNALAAYTFAKRR
                     FPGRNVIFLLFLATMMVPPQVTLIPNYLVLKHVPLFGGNDLAGQGGHGWLDSYWGLIL
                     PGAVSAFGIFLLRQYMMSIPDELLDAARVDGAGEFRVFWKVVIPLCGPALAATAIFTF
                     TYAWEDFL"
BASE COUNT          212 a          548 c          511 g          317 t
ORIGIN      
        1 tcctgttctc catcttcacc gtgttcgccc tcggcttcgc gttctacctg accttccgcg
       61 agtggaacat cctggagccg gagaagccgt tcatcgggct gcagaactac cgggacatga
      121 tcggcgacga ggacttccgg cgctcggtga tcaacacgtt ctacttcacc ggtgcctcgg
      181 tgccgctcgg gatggcggtc gggctcggcg tcgcattgct gctcaaccag gcgctgtggg
      241 gccgcacgct gctgcgcacg ctgttcttcc tgccggtcgt cacccccttc gtggtcgccg
      301 cgatcgtctg gaagtggctc tacaacggcg atttcgggcc gatcaactac tacctgctca
      361 agacccatct gatcagcgag ccgctgctgt ggctgtcgga caagaacctg gcgatgccgg
      421 ccgtggtgct gatgagcgtg tggaccagcg tgggcttctc gatggtcgtc tacctggcgg
      481 ccctgcaggg cgtgccggcc gagctctatg agtcggcgcg gatcgacggg gccggcgcgt
      541 gggcccggtt ccggtacgtg accctgccga tgctggcgcc ctcgacgctg ttcctcatgg
      601 tgatgggcat catcggctcg ttccaggtgt tcacccagat ctacatcatg accagcggcg
      661 ggccggtgaa ccgcacgtcg accatggtct attacatcta cgagtcagcc ttcaagttct
      721 acgagatggg ttacgcgagc accctcgcgt acgccctgtt cgtgctgctg ctggtgttca
      781 ccgtgatcca gctccgcctc taccggaggg ccgacctgtg acggcgacga tcccgcgacc
      841 ggcggtggag acctcaccgc ccggcagcgc gaagccgcgg cgccgccgga gcgcgcggag
      901 ccggctctgg atggcggtgt cgtacgcgat cctggtgccc ggcgcgatcc tcttcgtcgc
      961 gccgttcgcc tggctggtca gcgcgtcgtt ccagcacgtc ggcgacatct tctcctggcc
     1021 gccgcagtgg ataccgcaga acccgacgct ggacggctac aagggcttct tcggcatcgg
     1081 ccggccgggc gagctggccc agttcaacga gggcgcctgg cgctggttcg tcaacagcgc
     1141 gttcgtggcc gtctcggtca ccgtgctcca gctgttcttc aacgcgctgg ccgcgtacac
     1201 cttcgccaag cgccggttcc ccggccgcaa cgtcatcttc ctgctgttcc tggccaccat
     1261 gatggtgccg ccgcaggtca cgctgatccc caactacctg gtgctcaagc acgtcccgct
     1321 gttcggcggc aacgacctgg ccggccaggg cgggcacggc tggctcgact cgtactgggg
     1381 tctcatcctg cccggcgcgg tgagcgcgtt cggcatcttc ctgctgcgcc agtacatgat
     1441 gtccatcccg gacgaactcc tggacgcggc gcgggtggac ggcgccggcg agttccgggt
     1501 gttctggaag gtggtgatcc cgctgtgcgg cccggcgctc gccgccaccg ccatcttcac
     1561 cttcacctac gcctgggagg acttcctc
//
LOCUS       DATADN010000963         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_574401, whole genome shotgun sequence.
ACCESSION   DATADN010000963 DATADN010000000
VERSION     DATADN010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574401"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VHH53_12480"
     CDS             complement(<1..495)
                     /locus_tag="VHH53_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX2290979.1"
                     /translation="MPAGSTISQTSGPQRKGGLGRGLAALIPTGPVGSAVSELASRER
                     HHGRSDVASGRVAGAVYQEVPIGAIVPNPKQPRQSFDEESLAELEHSIREFGLMQPIV
                     VRPLPAEPAIGQLPAEPAIGQLPADPATGHPPADPAIAQLRTVSYELVMGERRWRAAQ
                     QAGLD"
     gene            complement(495..1454)
                     /locus_tag="VHH53_12485"
     CDS             complement(495..1454)
                     /locus_tag="VHH53_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX2290980.1"
                     /translation="MSYPAPPPVAAQPKDVGWTPICEEAERATRVQHPTDQLPRPFRR
                     RVLAVANQKGGVGKTTSTVNLAAGLALHGLRTLVIDLDPQGNASTALGVEHRSGTRSV
                     YEMLIGELGITDAVVVSPQSPNLLCIPATIDLAGSEIELVSMVARESRLSRALQGWAL
                     DELAVDYVFIDCPPSLGLLTVNALVAAPEVLIPIQCEYYALEGLGQLLRNIELVQAHL
                     NPALRVSTILLTMYDGRTKLADQVTSEVRNHFGDQVLRTVIPRSVKVSEAPGFGQTVL
                     IYDPGSRGALSYLDAAREIALQPDDPRHDYRDGPPPGTQEVRL"
BASE COUNT          256 a          538 c          548 g          256 t
ORIGIN      
        1 gtccagcccg gcttgttgcg ccgctcgcca ccgccgctca cccatgacca gctcatagga
       61 aacggtgcgc agctgcgcga tagccgggtc cgccggcggg tgtccggtcg ccgggtccgc
      121 cggcagttgt ccgatcgccg gctccgccgg cagttgtccg atcgccggct ccgccggcag
      181 tggccggacc acgatgggct gcatcagccc gaactcacgg atcgaatgct cgagctcagc
      241 cagggactcc tcatcgaagg actgccgagg ctgcttgggg ttgggcacga tggccccaat
      301 aggcacctct tggtagacgg cccccgcgac acggccactg gcgacgtccg agcgcccgtg
      361 gtgacgctca cgtgacgcca gttcgctcac cgcggagccg accggcccgg tggggatcaa
      421 cgcggcgagg ccgcggccga ggccgccttt gcgttgcggc ccagaggttt gcgagatcgt
      481 gctaccagcc ggcatcagag acgcacctcc tgggtacctg gaggggggcc gtcccgatag
      541 tcatgtcggg gatcgtcggg ctgcagcgcg atttcccgcg cggcgtccag gtagctcagc
      601 gccccccggg agccggggtc atagatcagc acggtctgcc cgaagcccgg ggcttcggag
      661 accttcacgc tgcgcggaat caccgtacgc agcacctggt caccgaaatg gttacggacc
      721 tcggaggtca cctgatccgc cagcttcgtg cgcccgtcgt acatcgtcag caggatcgtg
      781 gacacgcgca gtgcgggatt gaggtgcgcc tgcaccaact cgatgttgcg caacagctgg
      841 ccaagcccct cgagggcgta gtactcgcac tggatgggga tcagcacttc cggtgccgca
      901 accagtgcat tcaccgtgag caggcccaac gatggcgggc aatcgataaa gacgtagtcg
      961 acggccagct catcgagcgc ccaaccttgc agcgcccggg acaggcgtga ctcccgggcc
     1021 accatgctca ccagctcgat ctcggaaccc gcgaggtcga tggtggcggg gatacagagc
     1081 aggttcggcg attgcgggct caccacaacg gcgtccgtga tgccgagctc gccaatcagc
     1141 atctcgtaca ccgagcgggt acccgagcgg tgctcaaccc cgagtgcggt cgaggcgttg
     1201 ccctgcggat ccaggtcgat caccagcgtg cgcagcccgt gcagagccag gcctgctgcc
     1261 aggttgaccg tgctcgtggt cttgccgacc ccgcccttct ggttagccac ggcaagcacc
     1321 cggcgccgga acgggcgggg aagctggtcg gtgggatgct gtacccgagt ggcgcgctca
     1381 gcctcctcgc agatcggcgt ccacccgacg tccttgggct gcgccgccac ggggggcggc
     1441 gcagggtagg acatcggcac tgcgcggtga agacgagctt cgctggtttg acacgtttcg
     1501 ctgggcgcgg tcgacagtgg agcacgagcg accgcgtgcg gtcgccgggt ttcacgtgaa
     1561 acgttgagca ctgcactgtc ctgcgcggtg ccgttctg
//
LOCUS       DATADN010000964         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1480693, whole genome shotgun sequence.
ACCESSION   DATADN010000964 DATADN010000000
VERSION     DATADN010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480693"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VHH53_12490"
     CDS             complement(<1..366)
                     /locus_tag="VHH53_12490"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX2290981.1"
                     /translation="MTRRNGRTAGRFDLAPDASALLHALLGPLAKPRPSSDTGPDPRT
                     TAERNGDALAEILNLAADSGTAPTEAGERPHLTVTINLRDLQAGLGHAVLGDSDLDAA
                     SARRIACDSRVIPAVLGTNS"
     gene            444..1796
                     /locus_tag="VHH53_12495"
     CDS             444..1796
                     /locus_tag="VHH53_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012751064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2290982.1"
                     /translation="MTTERSQSAGEPAARQVMRELAAAGVLDEVMGRVDRDGLALTGE
                     GGFLPELVKAVLERGLQAELSEHLGYEKGDPAGRSSPNSRNGTTPKTLVSEVGPVPLA
                     VPRDRNGSFEPRLVPKGERRAGGLDDMIISLYAGGMTVRDIQHHLARTIGTELSHDTI
                     SKITDTVLEEVKAWQSRPLEELYPIIYLDALVVKVRDGHTVRNKAAHLAVGVDLDGVK
                     HVLGIWVQANEGAKFWAGVCAELRNRGIRDVLIVCCDGLTGFPEAVEATWPASTVQTC
                     TVHLIRASMRFISYADRKKVAAALKPIYTAPTIEAAEAELMAFAESDLGRRYPACVST
                     WRAAWQRFVPFLAFPPELRRIIYTTNAIESLNYQLRKIIKNRGHFPTDDAVVKLLWLA
                     IRDIEDKRARQRAAERGVPAADRRAPARLVEGAVVQGWRQALGALALTYPDRLAPYLG
                     "
BASE COUNT          312 a          660 c          723 g          320 t
ORIGIN      
        1 ggaattcgtt cccaggacgg caggaatgac gcggctgtcg caggcgattc gccgcgccga
       61 tgcagcgtcg aggtcggagt caccgaggac ggcgtggccg aggccggcct ggaggtcgcg
      121 gaggttgatg gtgacggtga ggtggggtcg ttcgccggct tcggtgggcg cggtgccact
      181 gtcggcggcg aggttcagga tttcggcgag cgcgtcccca ttgcgttcgg ccgtggtccg
      241 cgggtcgggt ccggtgtcgc tgctgggccg tggtttggcc agtggtccga gcagggcgtg
      301 gagcagtgcg gaggcgtcgg gtgcgaggtc gaaccggccg gcggtgcggc cgttgcgcct
      361 ggtcaccagg tgcagctcgt tgaccggctc gggggcgtgt cagatggtct gtgtacaaga
      421 tcgaatggag tacacaggtg gccatgacaa cggagcggtc gcagtctgct ggggagccgg
      481 cggcgcggca ggtgatgcgg gagctcgcgg cggccggggt gctggatgag gtgatgggcc
      541 gggttgaccg tgacggcctg gcgctgaccg gcgaaggagg gttcctgccg gagctggtca
      601 aggctgtgct cgagcgcgga ttgcaggccg agctgagcga gcacctcggg tatgagaagg
      661 gcgacccggc cggccgcagc agtccgaact cgcgtaacgg gacgacgccg aagacgctgg
      721 tcagtgaggt ggggccggtg ccgttggcgg tgccgcggga ccgcaacggc tcgttcgaac
      781 ccaggttggt gcccaagggt gagcgtcgcg ccggcggcct ggacgacatg atcatctcgt
      841 tgtatgcggg tgggatgacg gtgcgcgaca tccagcacca cctggcgcgc acgatcggca
      901 ccgagttgtc gcacgacacc atcagcaaga tcaccgacac ggtgttggag gaggtcaagg
      961 cctggcagag ccgcccgctg gaagagctct acccgatcat ttacctggac gcgctggtgg
     1021 tgaaggtccg tgacgggcac accgtgcgca acaaggccgc tcacctggcc gtcggcgtgg
     1081 acctggacgg cgtcaagcac gtcctgggta tctgggtgca agccaacgag ggggcgaagt
     1141 tctgggccgg ggtctgcgcg gagctgcgca accgcggcat ccgcgacgtg ctcatcgtct
     1201 gctgcgacgg cctgaccggg tttcccgagg cggtggaggc gacctggccg gcctcgacgg
     1261 tgcagacctg cacggtgcat ctgatccggg cctcgatgcg gttcatctcc tacgccgatc
     1321 gcaagaaggt cgctgccgct ctcaagccca tctacaccgc gcccacgatc gaggccgccg
     1381 aggcggaact catggccttc gccgagtccg acctgggccg ccgctacccg gcctgcgtga
     1441 gcacctggcg ggccgcgtgg cagcggttcg tgccgttcct ggccttccca cccgagctgc
     1501 gccgcatcat ctacaccacc aatgcaatcg aatcgctgaa ctaccaactc cgcaagatca
     1561 tcaagaaccg cgggcacttc ccgaccgacg atgccgtggt caagctgttg tggctggcca
     1621 tccgcgacat cgaggacaaa cgcgcccgcc aacgcgccgc cgaacgcggc gtcccagccg
     1681 ccgaccgccg cgcccccgcc cgactcgtcg aaggcgccgt cgtccagggc tggcgccaag
     1741 cactcggcgc gctcgccctc acctaccccg accgactcgc cccctacctc ggctaatcaa
     1801 cccatgccca cacatacaca gacaacttga caggctccca tctcgacggc gtccacggtg
     1861 gaagtcgagg ccgcccggct gctcttggcc aagcttggtg tttccgccgc tgatcttctc
     1921 gccgcacacc tgacgcggcc gccggtgccg acgtttgccg agtacatccc ggtcgccgcc
     1981 gccgcggtca cctacggcac ccgccgggtc tatgg
//
LOCUS       DATADN010000965         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1181262, whole genome shotgun sequence.
ACCESSION   DATADN010000965 DATADN010000000
VERSION     DATADN010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181262"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1774)
                     /locus_tag="VHH53_12500"
     CDS             complement(2..1774)
                     /locus_tag="VHH53_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2290983.1"
                     /translation="MPQSDPTTLRKVLIANRGEIAIRVIRACRDAGLNSVAVYADDDR
                     DAPFVRLADEAFALRGAIPADSYLDQAKLLAVAARTGADAVHPGYGFLAEHADFAQAV
                     INAGLTWVGPAPQAIRDLGDKITARQLAAKAGAPLVPGTKDPVSGADEVVAFALEHGL
                     PVAIKAAFGGGGRGLKVAHTLEEIPERLDSAVREAVSAFGRGECFVERYLDKPRHVEA
                     QVLADQHGTIIVVGTRDCSLQRRHQKLVEEAPAPFLSDEQRRTIHEAARAICTEAAYT
                     GAGTVEFLVGLDGSISFLEVNTRLQVEHPVSEETTGLDLVREQFRIAAGEKLTITSDP
                     QPRGHAIEFRINAEDPARGFVPVPGTVTGFEPPSGPGVRLDAGVRAGSVIGGQFDSML
                     AKLIIVGADREQALERSRRALAEFTVEGLPTIIPFHRVIVDDPAFAATDGEHFTVHTR
                     WVETEFSAQLQPYGAPEQVPLPAPRQTMVVEVDGRRLEVTLPGDLALRGGDTGRARPR
                     KRGGGRAAAVSGNAVIAPMQGTVVKVAVSEGDAVDTGDLIVVLEAMKMENWVTAHQAG
                     IITGLTAQAGTSLTQGTVLCQITP"
BASE COUNT          286 a          622 c          630 g          294 t
ORIGIN      
        1 gctagggtgt gatctggcag aggacggtgc cttgggtgag cgaggtgccg gcttgcgccg
       61 tgagcccggt aatgatgcca gcctggtggg cggtgaccca gttctccatc ttcatcgcct
      121 cgagcaccac gatgaggtca ccggtgtcga ccgcatcgcc ctcagacacc gcgaccttga
      181 ccacggtgcc ctgcatggga gcgatgactg cgttgcccga caccgcagcc gcccgaccac
      241 cgccgcgctt gcggggtctg gccctgccgg tatcaccgcc tcgaagggcc agatcgcccg
      301 gcagcgtgac ttccagccgc cggccgtcga cctccacgac catggtttgc cgcggcgctg
      361 gcagtggcac ttgttcgggg gcaccgtagg gctgaagctg ggcggagaac tcggtctcga
      421 cccaccgggt gtgcacggtg aaatgctcgc cgtcggtggc ggcaaaggcg ggatcgtcga
      481 cgatcacccg gtgaaacggg atgatggtag gcaagccctc gacggtgaac tcggccagcg
      541 ctcgccggga cctctccagc gcctgctccc ggtccgcgcc gacgatgatg agtttggcca
      601 gcatcgagtc gaactgaccg ccgatcacgc taccggcacg caccccggcg tccagccgca
      661 cgcccgggcc cgacggtggc tcgaacccgg tcacggtgcc gggcacgggc acgaagcccc
      721 gggccgggtc ctcggcgttg atccggaact cgatcgcgtg gccacgcggc tgcggatcag
      781 aggtgatggt cagcttctcg ccggctgcga tccggaactg ctcgcgcacc agatcgagcc
      841 cggtggtctc ctccgacacc ggatgctcca cctgcagcct ggtgttcacc tcgagaaacg
      901 agatcgagcc atcgaggccg accaggaact cgacggtacc tgcgccggtg taagccgcct
      961 cggtgcagat cgcccgggcc gcctcatgaa tggtgcggcg ctgctcgtcg gacaggaaag
     1021 gagccggggc ctcctcgacc agcttctggt gccggcgctg cagtgaacaa tcccgggtgc
     1081 cgacgacgat gatggtgccg tgctggtcgg ccagtacctg cgcctcgaca tgccggggct
     1141 tgtccaggta gcgctctacg aagcactcgc cgcgaccgaa ggctgacacg gcctcgcgca
     1201 ccgcggagtc gagccgctcg gggatctcct cgagggtgtg ggcgaccttc aagccgcggc
     1261 caccaccacc gaaggcggcc ttgatcgcca ctggcaggcc gtgctccagc gcgaaggcca
     1321 ccacctcgtc agcgcctgat accggatcct tggtacctgg caccagcggt gcgcccgcct
     1381 tggcggcgag ctggcgtgcg gtgatcttgt cgcccaggtc acggatcgcc tgcggcgccg
     1441 gtccgaccca ggtcaatccg gcgttgatca ccgcttgggc gaagtctgca tgctcggcga
     1501 gaaagccata gccgggatgt accgcgtctg caccggtgcg ggcggcaacg gccagcaact
     1561 tggcttgatc gaggtagctg tctgcgggga ttgcaccgcg cagtgcgaac gcctcgtcgg
     1621 ccagccgcac gaacggcgcg tcgcggtcgt cgtcggcata gaccgcgacg ctgttcagcc
     1681 cggcatcccg gcaggcacgg atcacccgga tggcgatctc accgcggttg gcgatgagca
     1741 ccttgcgcag ggttgtcgga tcagactgag gcaccgtgcc tccgcggtgg tgaggtcgta
     1801 ttcgccgcag tctagggccg atgcgtggtc ag
//
LOCUS       DATADN010000966         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_574481, whole genome shotgun sequence.
ACCESSION   DATADN010000966 DATADN010000000
VERSION     DATADN010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574481"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VHH53_12505"
     CDS             <1..919
                     /locus_tag="VHH53_12505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2290984.1"
                     /translation="PVWTNREATTLREIPDRVVMVGGSAVGTELGQFLHRFGARMTIV
                     ERSATLLFREEPRVGEILGQRLRAEGIDVRTGATATEVRRDGADTVITLDDGAVLRCD
                     VVVVGAGRTPRTADLGLDTIGVTLGDKGQIRVDEHCRAADGLWAIGDVTAIMPFTHVA
                     KYQARVVADTILGRPRAASYRGIPRVVFTDPEVAAVGLTRAQADRQGLRTSAVELDLT
                     AALARPWIYEQDPRGHLGLLVDTDQGILLGAWAVAPMAGEWIHQAALAIRTRTAIEVL
                     LDQVAQFPTYSEGFLAALEELALNPTQHG"
     gene            1010..1504
                     /locus_tag="VHH53_12510"
     CDS             1010..1504
                     /locus_tag="VHH53_12510"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEX2290985.1"
                     /translation="MVLGFDRRQDAEEVFELGAHLQEHALIDLEDAALAWRDDKGRVR
                     IQQALPTVATGASTVGLAGALWGTLIGLLVLNPLAGLVVGGLAGAAAGAAAGALNDIG
                     ISDDLIRRIGEQLQPGKAAVIALIRRSTKDKVTDALKQYHPTVLHTNLSRDREQDLIQ
                     ALQS"
BASE COUNT          245 a          538 c          538 g          246 t
ORIGIN      
        1 cccggtgtgg accaaccggg aagccaccac cctgcgcgag atccccgacc gggtggttat
       61 ggtcggcggc agcgcagtgg gcactgaatt aggtcagttc ctgcaccgct tcggtgcccg
      121 gatgaccatt gtggagcggt cggccacgct gctgttccgg gaggaacccc gcgttggtga
      181 gatcctcggc cagcggctgc gcgctgaggg cattgacgtg cgcaccggcg ccaccgccac
      241 cgaggttcgc cgggacggcg ccgacacagt catcaccctc gacgacggcg ccgtgctgcg
      301 ctgcgatgtc gtggtcgtcg gcgcgggccg cactcctcgc actgccgacc tgggcctgga
      361 cacgattggc gtaacccttg gcgacaaggg ccagataagg gtcgatgagc actgtcgagc
      421 cgccgacggg ctgtgggcga ttggcgacgt caccgcgatc atgccgttca cccacgtcgc
      481 gaagtaccag gcccgcgtcg tcgccgacac catcctgggg cgcccgcgtg ctgctagcta
      541 ccggggcatt ccccgggtgg tgttcaccga ccccgaggtc gccgccgtcg gcctcacccg
      601 cgcccaggcc gaccggcagg gcctacgcac ttcggctgtt gagcttgacc ttaccgctgc
      661 gcttgcccgt ccctggatct atgagcaaga ccctcggggc catctcgggc tgcttgtcga
      721 caccgaccag ggcatcctgc ttggcgcctg ggccgtcgca ccgatggctg gcgaatggat
      781 ccaccaggcc gccctggcca tccgcacccg caccgcgatc gaggttctgc ttgaccaggt
      841 cgcgcagttc cccacctaca gcgagggttt cctcgccgcg ctggaggaac tcgccctgaa
      901 ccccacccaa cacgggtgat gtcgggacgt tgaaagcctg tcggtgagag cattacgaaa
      961 ggcaagctgc ctatcgcaga tgattgaggg ggccgatgtg gccgacttgg tggtgctcgg
     1021 cttcgaccgg cggcaggacg ccgaggaggt cttcgagctc ggcgcgcacc tgcaggagca
     1081 cgctcttatt gatctggagg acgccgcact ggcctggcgc gacgacaagg gcagggtgcg
     1141 catccagcag gcgctaccca ccgtggcgac gggcgcgtcc accgtcgggc tcgctggcgc
     1201 gctgtggggc acgttgatcg gcctgttggt gctgaacccc ctggcggggc tggtcgtggg
     1261 gggcctcgcg ggcgcggcgg cgggcgcggc ggcgggcgcg ctcaacgaca tcggcatcag
     1321 cgacgacctc atccgccgga tcggcgaaca gctgcaaccc ggcaaggctg cggtcatcgc
     1381 cctgatccgg cggtcgacca aggacaaggt gaccgacgcg ctcaagcagt accacccaac
     1441 cgtgctgcac accaacctga gccgcgaccg cgagcaggac ctcatccaag ctctgcagtc
     1501 ctgaccgggt aaccgccgag gctggtggac acttgaggca gcccgttctg agtcccggag
     1561 gctggag
//
LOCUS       DATADN010000967         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_720962, whole genome shotgun sequence.
ACCESSION   DATADN010000967 DATADN010000000
VERSION     DATADN010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720962"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VHH53_12515"
     CDS             <1..344
                     /locus_tag="VHH53_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2290986.1"
                     /translation="DFDMKVSVTIEAMPEQLPAPVSQAAYRTVQEALTNVRKHAVGAA
                     AAVRVVGQRDTLMVEVRNDPPHTRPSGLPSGGHGLVGLRERTGLLGGTFHAGPTPEGG
                     FRVEATYPIQP"
     gene            486..1814
                     /locus_tag="VHH53_12520"
     CDS             486..1814
                     /locus_tag="VHH53_12520"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2290987.1"
                     /translation="MPHGRALMRGDRRRNERLARLRSIVCREFAIVAVDLASDRQAVA
                     VTDHDSVVLGRRMFSGDAWVIDEILDWAEPIAVAAGFAGVVLGCEPTGHRWKPLLDRA
                     RARGVELVCVNPMLVHRGREEEDFTRDRSDFKDASIIAKRVAELRCYVPFVLEGHWCR
                     LRHLGARRNDQLVAAGSARQRLRDLLECAWPAVLTTAADPLDAITWRAAMAVSTQPQE
                     ILAMGLDAFAAAVGEQLPRWGGTRRNLRILHAIWQAAQAPGGVATERAAACERAAYAL
                     ADYHHALVQLADVEARMVSVVDALELTELVTSIPGLSAVGAAAILAETGDPARFDCAR
                     TWVKHAGLCPRANESGTFHGTTKISGRGRPALRTAAWRAIWAGWRHNPVYLARYTHLT
                     TRTRNPLKPGQARAALAAALLRQLFVIVTRRIAWDPAIAAGTKEVAPQAA"
BASE COUNT          340 a          798 c          803 g          321 t
ORIGIN      
        1 gggacttcga catgaaggtg agcgtgacca tcgaggcgat gccggagcag ctgccggcgc
       61 cggtgtcgca ggcggcctac cggaccgtcc aggaggcgct gaccaacgtg cgcaagcacg
      121 cggtgggcgc ggccgcggcg gtgcgggtgg tcggccagcg ggacaccctg atggtcgagg
      181 tgcgcaacga cccgccgcac acccggccgt ccgggctgcc gtccggtggg catgggctgg
      241 ttggcctacg ggaacggacc ggcctactcg gcggcacgtt ccacgccgga ccgacccccg
      301 agggcgggtt tcgagtggag gccacctacc ccatccagcc ctgactgggc gcaacggtgg
      361 ggggccgacc cacctacgaa cgcaacctag taaggaccgg tagcagttgg tccggagtcg
      421 atgacgcagc atctgcttgt ggctgtggtt cgggttttgg tccagctatt accgaggagg
      481 atgttgtgcc ccatggcagg gcgctgatgc gtggtgatcg tcgacgaaac gagcggttgg
      541 ctcggttgcg gtcgattgtg tgccgtgaat tcgcgatcgt ggcggtggat ctggcgtcgg
      601 acaggcaggc ggtggcggtc accgatcacg actcggtggt gctgggccgg cggatgttca
      661 gcggggatgc gtgggtgatc gacgagatcc tggactgggc cgagccgatc gccgtggcgg
      721 cggggttcgc cggggtggtg ctggggtgtg agccgaccgg gcatcgctgg aagccgctgt
      781 tggaccgggc ccgcgcccgt ggggtggagc tggtgtgtgt gaatccgatg ctggtgcacc
      841 gcggccggga ggaggaggac ttcacccggg accggtcgga cttcaaagac gccagcatca
      901 tcgccaagcg agtggccgag ctgcgctgct atgtgccctt cgtgttggag ggccactggt
      961 gccggttgcg gcacctcggg gcgcgccgca acgaccagct cgtcgcggcc ggctcagcgc
     1021 ggcagcggct gcgggacttg ctggagtgtg cctggccggc ggtgctgacc accgcggcgg
     1081 atccgttgga tgcgatcacg tggcgggcgg cgatggcggt gtccactcag ccgcaggaga
     1141 tcctggcgat ggggttggat gccttcgctg cggcggtggg cgagcaactg ccccgctggg
     1201 gcggtacccg ccgcaacctg cggatcctgc acgcaatctg gcaggcggcg caggctcccg
     1261 gcggggtggc caccgagcgg gccgcggcct gcgaacgcgc cgcctacgcc ctggccgact
     1321 accaccacgc gctggtccaa ctcgccgacg tcgaggcccg catggtcagc gtcgtcgatg
     1381 cgctggagct gaccgaactg gtcaccagca tccctggcct gtcggccgtc ggggccgccg
     1441 ccatcctcgc cgagaccggt gacccggccc gcttcgactg cgcccgcacc tgggtcaaac
     1501 acgccgggtt gtgcccgcgg gccaacgaat ccggcacctt ccacggcacc accaagatct
     1561 ccggccgcgg ccggcccgcg ctgcgcaccg ccgcctggcg cgccatctgg gcggggtggc
     1621 ggcacaaccc ggtctacctc gcccgctaca cccacctgac cacccgcacc cgcaaccccc
     1681 tcaaacccgg ccaggcccgc gccgcactgg ccgccgcgct gctgcggcag ctgttcgtca
     1741 tcgtcacccg ccgcatcgcg tgggaccccg ccatcgccgc cggtaccaag gaggtggcgc
     1801 cccaggccgc atagcgcgac actggcccgg gcggggcgaa cccgactcaa ccttgggcag
     1861 aacccgaccc tgagcagggg cgccccgccc accctgcacc caagctcgca tgaaggctgt
     1921 tgggacaaac ccggttgttc gctaggtcga gcacgcaggg tgggcacccg cacccgccac
     1981 caccggccac tcgcaccacc caccacacca acacccacca tgatcaaccc cgcccacccg
     2041 cggggccgat cccgcacgcc caaccaccac aaacccgaca cttgactccg cgctcatagg
     2101 ctgctcaggg gaccgtaacg caacccagcc acaatcaacc cgatgtccgc accgttccct
     2161 ggaacgtgac gacagcagtt aatcgcggag aggcggattg gcgcgggcct tggcccgggc
     2221 ccgctcgcgg gcccgggtgg tgccgtccag cacgaccttg cg
//
LOCUS       DATADN010000968         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_102663, whole genome shotgun sequence.
ACCESSION   DATADN010000968 DATADN010000000
VERSION     DATADN010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102663"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2078
                     /gene="pheT"
                     /locus_tag="VHH53_12525"
     CDS             <1..>2078
                     /gene="pheT"
                     /locus_tag="VHH53_12525"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2290988.1"
                     /translation="SKPIRYCLVDDGELAPRGIVCGATNFNVDDLVVVARPGVVLPGD
                     FVIASRTTYGRTSDGMICSAAELGLGEDHTGIMVLPAGTAIPGASAVELLGLDDAVIE
                     LAITPDRGYCLSMRGLARELAIAFDAPYGDPSLLEVPQTSSTAWPVHVEDEHGCRRFV
                     VRWVSGVDPAAASPWWMRRRLQLAGLRPISLAVDVTNYVMLELGQPLHAFDAAKLDGD
                     LVVRRATEGEKLTTLDGVCRELDPDDMVICDASGPVSLAAVMGGESTEVGGQTTSLLL
                     EAATWDPASVARSARRHKLPSEAARRFERGVDPALPPVAAELAATLLSRYGGGEIQQG
                     RTDVGTLPRPKPVTMPIDLPDRVAGRRYENGATVRRLSQIGCAVTLGGTNGTTTVTVV
                     PPSWRPDLSQPADLVEEVLRLEGYDTIPSELPPAPPGQGLSAEQRRRRTISRALAAAG
                     YVEVLPSPFVSAGTWDALGLPGDDPRRHTATLLNPLETDRDQLSTTLLPGLLEALVRN
                     VARGQRDVLLYGMAPVVQPRAATTTMPHVRVDRRPSDDQLAALNRALPVQPLHVGVVL
                     AGQYERAGWWNTVRAAIWADAVQAARLVGHAAGVDIAVRAAQTAPWHPGRCAQLVVDG
                     RVLGHAGELHPAVIEALGLPARTCAMELELDALPLTERRPAPVVSPYPPVLQDVALVV
                     DAAVPAAELT"
BASE COUNT          281 a          699 c          764 g          334 t
ORIGIN      
        1 tctccaagcc gatccggtac tgcctcgtcg acgacggtga gctggcgccg cgcgggatcg
       61 tctgcggtgc caccaacttc aacgtcgacg atctggtggt ggtcgcgcgg ccgggggtgg
      121 tgctgccagg cgactttgtg atcgcgtcgc gaaccaccta cggccggaca tccgacggga
      181 tgatctgctc ggctgccgag ctcgggctcg gtgaggacca caccggcatc atggtgctcc
      241 cagcgggcac cgcgatcccg ggggcaagcg ctgtcgaact actcggcctg gatgacgcgg
      301 tgatcgagtt ggcgatcacc ccagaccgcg gctactgcct gtcgatgcgt gggctggcgc
      361 gcgagctggc catcgccttc gacgctccct acggggatcc cagcctgctg gaggtgccgc
      421 agacgtcgag taccgcctgg ccggtgcacg tcgaagacga gcacggttgc cgccggttcg
      481 tggtccgctg ggtatcgggt gtggaccctg cggccgcgtc cccatggtgg atgcgccgca
      541 gattgcagct ggctgggctg cggccgatct cgctcgccgt cgacgtcacc aattacgtga
      601 tgctggagct cgggcagccg ctgcatgcct ttgacgccgc caagttggac ggtgacctcg
      661 tcgtccggcg tgctactgag ggcgagaagc tgaccacgtt ggacggggta tgccgggagc
      721 tggatcccga cgacatggtg atctgcgatg ccagcggtcc ggtgtcgttg gcggcggtga
      781 tgggtggcga aagcaccgag gtcggtgggc agaccaccag cctgctgctg gaggcggcga
      841 cctgggatcc ggcctcggtg gcccgcagcg cccggcggca caagttaccc agcgaggccg
      901 cccggcgctt cgaacgtggg gtggatcctg ccctgccccc ggtggctgcc gagctcgcgg
      961 cgacgctgct gagtcgctat ggcggcggtg agatccagca aggccgcacc gatgtgggaa
     1021 cgcttccgcg gcccaagcct gtgacgatgc cgatcgatct gccggatcgg gtggcggggc
     1081 ggcgttatga gaacggtgcc acggtccggc ggctgtccca gatcggctgt gccgtgacgc
     1141 tcggcggaac caatggcacc acgacggtca ccgtggtccc gccgtcctgg cggcccgatc
     1201 tgtcccaacc cgccgacctc gtcgaggagg tgctgcggct ggagggctac gacaccatcc
     1261 cgtctgagct gccgccggcg ccacccggtc agggcctgtc cgccgagcag cgtcgccgcc
     1321 ggacgatctc acgggcgttg gccgccgccg ggtacgtgga ggtgctgccc agccccttcg
     1381 tctcagccgg gacctgggat gcattgggct tgcccggcga cgatccccgg cggcacaccg
     1441 cgacgctgct caacccgctg gagaccgacc gcgatcagct gtccacgacg ctgctgcccg
     1501 gcttgctcga agccctggtg cgcaacgtcg cccgcggcca acgcgacgtg ctgctctacg
     1561 gcatggcccc ggtcgtgcag ccgcgggccg ctaccacgac gatgccgcac gtgcgggtgg
     1621 accgacgacc aagcgacgac cagctcgccg cgctgaaccg tgcgttgccc gtgcagccac
     1681 tgcacgtggg tgtcgtactc gccgggcagt acgagcgtgc cggctggtgg aacacggtca
     1741 gggcagcgat ctgggccgat gccgtacagg ccgcgcgact ggtcggccac gccgcgggag
     1801 tggacattgc ggtgcgtgcc gcccagaccg cgccgtggca tcccggccgg tgcgcgcagc
     1861 tggtggtgga tggtcgcgtg ctgggccacg ccggggagtt gcaccccgcg gtgatcgagg
     1921 cgcttgggct accggcgcgg acctgtgcga tggagcttga actcgatgcg ttgccgctga
     1981 ccgagcggcg gcccgcaccg gtggtgtcgc cctacccgcc ggtattgcag gacgtcgcat
     2041 tggtggtgga tgcggccgtg cctgccgccg agctgacg
//
LOCUS       DATADN010000969         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1181427, whole genome shotgun sequence.
ACCESSION   DATADN010000969 DATADN010000000
VERSION     DATADN010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181427"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1179)
                     /locus_tag="VHH53_12530"
     CDS             complement(34..1179)
                     /locus_tag="VHH53_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2290989.1"
                     /translation="MTPTFRLGRIAGVQVGANWSVLVILALIVVGLAAGRLPLVAPGR
                     SALTYAVTGALSGLVFLASLLAHEVSHAVVARRNGIAVEGITLWLFGGVAQLRGEAAS
                     PGADLRIAGIGPVVSALLGGGFFAAGAVVAAIDGGPLLLSALNWLAVINVVLAAFNLI
                     PAAPLDGGRVLRAVLWRVTGDRRRAAVIATRAGVMVGFVLIGLGLAQVVLGRGFGGLW
                     LALIGWFLVNAARAEEQQTVWQSALAGIHVADVMTPDPVTASPELTVQQFIDDYLMRH
                     RFSTIPLVDSAGQLAGLVTLNRIRQVPDGQRAATRLADIACRPADLPAAAAQEPLTDL
                     LPRMAGCSDGRAVVLDPQRRLVGIVSPSDVARAVQAAELRRPVSGRT"
     gene            1607..2071
                     /locus_tag="VHH53_12535"
     CDS             1607..2071
                     /locus_tag="VHH53_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290990.1"
                     /translation="MTVRYAAGVVAMAVLPLVACGSGDETIRNPAELNPPAVVTEQPN
                     LNEQGQGGVAQENLEGRLGQQVRVTGEVATIISPNAFTIGGEEIGENPILVVGANLPP
                     SLVEGQRVRVSGTVKEFQVRGYEEDFDLDLVDQEFEDFDGDPAIQANSVTTV"
BASE COUNT          429 a          758 c          716 g          335 t
ORIGIN      
        1 cataagccgt gccgcggcag ccgcttgagt ggctcaggtc ctgccactga ccggtctgcg
       61 cagttcagcg gcctggacgg cgcgtgccac gtcggacggg gacacgatgc cgaccaaccg
      121 gcgctgcggg tcaagcacaa cagcgcggcc gtcggaacaa ccggccatcc tcggcagcag
      181 gtcggtaagc ggttcctggg ccgccgcggc cgggaggtcc gcgggtcggc acgcgatatc
      241 agcgagccgg gtcgccgcgc gctgcccgtc gggcacttgc cggatccggt tcaaggtcac
      301 gagtccggcg agctggccgg ccgagtccac cagtgggatc gtgctgaacc ggtgccgcat
      361 gaggtagtcg tcgatgaact gctgcacggt gagttcgggt gacgcggtga ccgggtctgg
      421 cgtcatcaca tcggcaacgt ggatcccggc gagtgcggac tgccagacag tctgctgctc
      481 ctcggcgcgg gccgcattca ccaggaacca cccgatcaag gccagccaca accccccgaa
      541 cccgcgcccc agcactactt gtgccagccc gagcccgatc agcacgaaac cgaccattac
      601 gccggcccga gtcgcaatca ccgccgcccg tcgccgatca ccggttaccc gccacagcac
      661 cgcgcgcagt acccggccac catccagcgg tgcggccgga atcagattga acgctgctag
      721 gaccacattg atcaccgcga gccagttcaa tgcggaaagc aacagcggcc caccgtcgat
      781 ggcagcgacc accgcgcccg cagcgaagaa gccgccgccc aatagagcgc tcaccaccgg
      841 tccgataccg gcaatgcgca ggtcagcacc cggactggcc gcctccccgc gcaattgggc
      901 gacgccgccg aacagccaca gggtgatgcc ctccacggct atcccgttgc gccgggccac
      961 cacggcgtgt gagacctcat gggctagcag cgacgccagg aacaccagcc cgctaagcgc
     1021 cccggtcaca gcgtaggtca aagccgaccg tcccggcgcg accaagggca gccgtccggc
     1081 agccagcccg accacaatca acgccagaat gaccagcaca ctccagttcg caccgacctg
     1141 gacaccagca atgcgcccga gccggaaggt cggtgtcact gctcggtccg cggccgcgtg
     1201 cagagatacc gccgcgtccc atgatcaacc tgtccgggat atttcggcat gggacatcct
     1261 tcgcatcgaa atgtcgtaac gcgttcatac ctcggtttcc cacggacggc cgggggtaat
     1321 catcggccat ggtggatcac gagaaccccg tcccgctata acctcgtgcg gaccgctgcg
     1381 ctggccagcg cggtcggtgc cccctcgacg cggggcctcc tcgacgtagg cgcacctcac
     1441 catcggctat attggcgcaa cgcggagctg gcgacgctct ccctgaaggg agactttcac
     1501 ttccgcgata ccggggcagc gccatagcgc aaaggcgcga gaacacgaac ggctagtttc
     1561 gcggaaatgc ttacttttgt tgtgaccgat gaggggtgga gcttcaatga ctgtgcgtta
     1621 cgctgccggc gtggttgcca tggcggttct tccactggtc gcctgcggca gcggcgatga
     1681 gacgatccgt aatcctgcag agctcaatcc gcccgcggtg gtgacagagc agccgaactt
     1741 gaatgaacag ggccaagggg gcgtagccca ggaaaacctc gagggccgcc tcggacagca
     1801 ggtacgagtg accggtgagg tcgccacgat catctcaccg aacgcgttca ccattggcgg
     1861 tgaggaaatc ggagagaacc ccatcctcgt cgtgggagcc aacctgcccc ccagcctggt
     1921 ggaagggcag cgtgtgcgag tgagcggcac ggtcaaggag ttccaggtac gcggttacga
     1981 ggaggatttc gatctggacc tggttgacca ggagttcgag gacttcgacg gcgacccggc
     2041 gattcaggcc aactcagtca ccacagtctg acaacgctca ctgctcgcct gggccggcct
     2101 ggtcgcctag gcggtccggc gccgcagtgg caggcggcgg agtacgaacg ctgcagaggg
     2161 cagttgtggt ctccaacgtt gccgtcaagc gataccgtcg ggtttgtccg ttcgcaggtc
     2221 gccaggagac aaccgatg
//
LOCUS       DATADN010000970         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1181465, whole genome shotgun sequence.
ACCESSION   DATADN010000970 DATADN010000000
VERSION     DATADN010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181465"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VHH53_12540"
     CDS             <1..495
                     /locus_tag="VHH53_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2290991.1"
                     /translation="ALTSQVQGALEDAGLEAGLLELEITESLLMKNVEDNVAMLRALS
                     GAGVRIAIDDFGTGYSNFSYLRRLPMHELKLAGSFAVSLHSPDADLVDEQIVATLVSL
                     AHALDLTVTAEGVETDVQLERFRTLGCDAGQGSLFGLPGAAEQIDTRLRAATSLCDVR
                     KKRR"
     gene            492..686
                     /locus_tag="VHH53_12545"
     CDS             492..686
                     /locus_tag="VHH53_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX2290992.1"
                     /translation="MTELDTVDIAAALLRVVVGLTLVAHGYNHLWGPGGLAGTTRWFG
                     SLSLRPPKLHAVLSAAGELV"
     gene            complement(762..1619)
                     /locus_tag="VHH53_12550"
     CDS             complement(762..1619)
                     /locus_tag="VHH53_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2290993.1"
                     /translation="MPLLPASLMEPLWVEFAALIESADRPEFCPTHPWGCHRRRVPDR
                     VVFDHVIAALVHGTGYERLASPSCSDRTIRRRLTEWAQAGLGQQLLQIGLKAYDRMIG
                     LELDNLSVDGSITKSPCGGEVSRRSPVDRGKQGTKRSVACDGNGIPLSVLAARANDHD
                     SPLLAPTLAGIPDMIGPLPDQPRMHLDRGYDSTKTRDLLEILGYHAEIAVKGTPAPIQ
                     ASKRWPVERTHSWMNGYGKLRRFTDKPKTIVEFYLHLAAALTVIRRLINRARTLYRWP
                     TRPATRRLR"
BASE COUNT          316 a          586 c          686 g          359 t
ORIGIN      
        1 gccctcacga gccaggtgca gggcgcgctc gaggatgccg gcctggaggc ggggctcctc
       61 gagctcgaga tcaccgaaag cctgctgatg aagaacgtcg aggacaacgt cgcgatgctg
      121 cgcgcgctct cgggcgccgg cgtgcgcatc gccatcgacg acttcggcac cggctactcc
      181 aacttctcct atctgcgccg actgccgatg catgagctca agctggctgg ttcgttcgcc
      241 gtgagcttgc attctcccga tgcggacctc gtggacgagc agatcgtggc caccttggtg
      301 agcctggcac atgccttgga cctgaccgtg accgccgagg gtgtggagac cgatgtgcag
      361 ctcgagcggt tccggacgct ggggtgtgac gcaggtcagg ggtcattgtt cggcttacct
      421 ggcgctgctg agcagattga tacccggctg cgggcggcta cgtcactctg cgatgtccgc
      481 aagaagaggc gatgaccgag ttagacaccg tcgatatcgc ggctgcgctg ctgcgtgttg
      541 tcgtaggact cacgctggtg gcgcacgggt acaaccatct gtggggaccc ggcgggctgg
      601 ccggtaccac gcgctggttc ggctcgctga gcctgcggcc gccgaagctg cacgcggtgc
      661 tgtccgccgc cggagagctt gtttaaggaa ggctatgagt acgtcctcat ggtcggcgtt
      721 gtctgcgcag tgatcggctt agagcgaccg gcaaatgatc gttagcggag gcgtcgggta
      781 gcgggtctag ttggccagcg gtagagggtg cgggctcggt tgatcaggcg ccggatcacg
      841 gtgagagcgg ccgcgaggtg gaggtagaac tcgacgatgg tcttgggttt gtcggtgaac
      901 cggcggagct tgccgtagcc gttcatccag gagtgcgtgc gttccaccgg ccagcgcttg
      961 ctggcctgga tgggagcggg tgtgcccttg accgcgatct cggcgtggta gcccaagatc
     1021 tcgagcaggt cccgggtctt ggtcgagtcg tagccgcggt ctaggtgcat ccgcggctgg
     1081 tcgggcagcg gcccgatcat gtcgggaatt ccggccaggg tgggcgccag cagtggtgag
     1141 tcgtggtcgt tggcgcgggc ggcgagcaca gacaacggga tcccgttacc gtcacaggcc
     1201 acggatcgtt tggtgccctg tttgccccgg tccacggggc tgcgtcggga cacctcacca
     1261 ccgcagggtg acttggtgat cgacccgtcg acggacaggt tgtcaagctc cagaccgatc
     1321 atccggtcgt aggccttcaa cccgatctgc agcaattgct ggccgagccc ggcctgggcc
     1381 cactcggtca gccgacgacg gatggtgcga tccgagcagc tcgggctggc caggcgttca
     1441 tagccagtgc cgtgtaccaa cgccgcgatc acatggtcga acacgactcg gtctgggacc
     1501 cggcgacggt ggcagcccca gggatgggtt gggcagaact cggggcggtc agccgactcg
     1561 atcagggcag cgaactcgac ccacagcggc tccatcaggg atgctggcaa cagaggcacg
     1621 gaccctccgg gtgatcgtta ggacttcagc aatcccatga tcacttgtag gtccgtgcct
     1681 caacctgtag cgacacacct catcctcatt tgccggtcgc tcttactcgg tccgggcacc
     1741 gcctcggtgg accgcctgct gggcatcgac gaagcgctgg ctggcgcagt ggggatcggc
     1801 cctcacagtc ggacttggag tgctcggcgc cacagtgctg ctggcggcca cctggtggcc
     1861 ggatcactca cgagactgac gatccctgta cacagcgtgc gcgtacggtg taccgagcgt
     1921 gcggacacgg cagggaagca accgata
//
LOCUS       DATADN010000971         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1480930, whole genome shotgun sequence.
ACCESSION   DATADN010000971 DATADN010000000
VERSION     DATADN010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480930"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /gene="glgX"
                     /locus_tag="VHH53_12555"
     CDS             complement(<1..1523)
                     /gene="glgX"
                     /locus_tag="VHH53_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX2290994.1"
                     /translation="MTVPQPWPGAAYPLGATYDGAGTNFALFSEAADKVELCLFDSGD
                     AGATETRVRLPEVDGFVWHGYLPGVGPGQHYGYRVHGPYDPPAGQRCNPAKLLIDPYA
                     KAVSTGVDWDESLFGYQFADPEQRNDADSASHAPKSVVISPFFGWAEDRHPRIPYHET
                     VIYEAHVRGLTVAHPEIPPEMRGTYAGLAHPVMINHFTTLGITAVELMPVHQFITDDN
                     LAEKGLKNYWGYNTVAFLAPHNGYAARGTVGPRAHEVVSQFKAMVRELHAAGIEVILD
                     VVYNHTAEGNHLGPTLSMRGIDNPAYYRLVGNDPRYYMDYTGTGNSLNVRQPHTLQLI
                     MDSLRYWVSEMHVDGFRFDLASTLAREFYDVDRLSTFFDLVQQDPVISRVKLIAEPWD
                     VGPGGYQVGNFPPLWTEWNGKFRDTVRDFWRGAPGTLGEFGSRLTGSSDLYQADGRRP
                     HASINFVTAHDGFTLTDLVSYNDKHNEANGENGQDGADDNRSWNCGVEGLTDDPEVV"
     gene            complement(1632..1736)
                     /locus_tag="VHH53_12560"
     CDS             complement(1632..1736)
                     /locus_tag="VHH53_12560"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2290995.1"
                     /translation="MLLHQAVAQVRLMTGRDAPIAVMRQALDTAVRAR"
BASE COUNT          300 a          566 c          612 g          319 t
ORIGIN      
        1 tccactactt cggggtcgtc ggtgaggccc tccaccccgc agttccagga ccggttgtca
       61 tcggcgccgt cctgaccgtt ttcaccgttg gcctcattgt gtttgtcgtt gtaggagacc
      121 aggtcggtga gcgtgaaccc gtcgtgcgcg gtgacgaagt tgattgacgc gtgtggccgg
      181 cggccatcgg cctggtagag gtccgaggag ccggtcagcc gggaaccgaa ctcgcccagc
      241 gtgcccggcg cgccgcgcca gaagtcgcgc accgtgtcac ggaacttgcc gttccattcg
      301 gtccacagtg gggggaagtt gcccacctgg taaccccccg gcccgacgtc ccacggttcg
      361 gcgatcagct tcacccggct gatcaccgga tcctgctgaa ccaggtcgaa gaacgtggac
      421 aggcggtcca catcgtagaa ctcgcgggcc agcgtggagg cgaggtcgaa gcggaagccg
      481 tcgacgtgca tctcgctgac ccagtaccgc agcgagtcca tgatcagctg aagggtgtgc
      541 ggttgacgca cattgagcga gtttccggtg ccggtgtagt ccatgtaata gcgcgggtca
      601 ttgccgacca ggcggtagta ggccgggttg tcgatcccgc gcatcgacag cgtcggtccc
      661 aggtgattgc cctcggcagt gtggttgtag accacgtcga ggatcacctc gattcctgcg
      721 gcgtgcaact cccggaccat cgccttgaac tgcgagacga cctcatgagc ccgcggcccg
      781 acggtacccc tggcggcgta gccgttgtgg ggggccagga atgcgacggt gttgtatccc
      841 cagtagtttt tcaagccctt ctcggcgagg ttgtcgtcgg tgatgaactg gtgcaccggc
      901 atcagctcca ccgccgtgat acccagggtg gtgaaatgat taatcatcac gggatgggcg
      961 aggccggcgt aggtgccgcg catctccggg ggaatctcgg ggtgcgcgac ggtcagaccg
     1021 cgcacgtgtg cctcgtagat caccgtctcg tggtacggga tacgagggtg ccggtcctca
     1081 gcccacccga agaaggggct gatcaccacc gacttcggcg catggctggc ggagtcggcg
     1141 tcattgcgct gctcgggatc agcgaactgg tagccgaaca gcgactcgtc ccagtcgaca
     1201 ccggtggata ccgccttggc gtagggatcg atgagcagct tggcggggtt gcaccgttgc
     1261 ccagcgggtg gatcgtaggg gccgtgcacc cggtatccgt agtgctgacc tggccccaca
     1321 ccgggcagat agccgtgcca gacgaaccca tcgacctcgg gcaaccggac ccgtgtctcg
     1381 gtggcaccgg cgtcaccgct gtcgaacagg catagctcca ccttgtcagc ggcctcggag
     1441 aagagcgcga agttggtgcc tgcaccgtcg taggtggcac ccagcgggta ggccgcaccg
     1501 ggccacggct gggggacggt catcggtctc cttctccacg tctatgggtg actgccgcgc
     1561 accggatctg gggtcacggt atccgaacta ccggtgccac cgggcacggc gggtaacagc
     1621 tgcgagcaca cctagcgggc ccggacggcc gtatccaacg cttggcgcat caccgcgatc
     1681 ggcgcatcgc ggccggtcat cagccggact tgtgcgaccg cttgatgcag cagcaccgcc
     1741 agaccgctga cgatccggca gcccgcattg agtgcggacg cggccagcgg cgtgggc
//
LOCUS       DATADN010000972         3089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_861098, whole genome shotgun sequence.
ACCESSION   DATADN010000972 DATADN010000000
VERSION     DATADN010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861098"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VHH53_12565"
     CDS             complement(<1..283)
                     /locus_tag="VHH53_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HEX2290996.1"
                     /translation="MCRHLAYLGPPRDLATLLLDPPHSLLRQTYAPVDMRGGGTINVD
                     GFGAGWYPDGATTPIRYRRAVPMWSDPSFATLAREVRSSAVLAAVRSATP"
     gene            complement(286..1599)
                     /gene="egtB"
                     /locus_tag="VHH53_12570"
     CDS             complement(286..1599)
                     /gene="egtB"
                     /locus_tag="VHH53_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX2290997.1"
                     /translation="MSTAALRDHLAAELERTRARSTALTDAVDDGDLIRQHSKIMSPL
                     VWDLAHVGNQEELWLVRDVAGREPVRADIDHLYDAFKQPRSGRSALPLLTPVEARGYL
                     GEVRHKVLDALERTPLEGSRLVTHGFAFGMIVQHEQQHDETMLATHQLRVGPAALHAP
                     APAPQRATDLPAEVLIPAGEFTMGTSTDPWALDNERPAHRAYTDAYWLDTTPVTNGEY
                     QRFLADRGYDDPRWWSERGWAHRCAADLVAPQFWYFDGGSWWRTRFGLTEPVPPDEPV
                     LHVSFFEAQAYAAWAGKRLPTEVEWEKAARFDPASGRSRRFPWGDEDPTPAHANLGQR
                     HLQPALAGSYPAGASPLGVHQLMGDVWEWTSSGFSGYPGFAPFPYREYSEVFLGGDYR
                     MLRGGSFGTDAATCRATFRNWDHPIRRQIFTGFRCARNAHPGELA"
     gene            complement(1617..2942)
                     /gene="egtA"
                     /locus_tag="VHH53_12575"
     CDS             complement(1617..2942)
                     /gene="egtA"
                     /locus_tag="VHH53_12575"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis glutamate--cysteine
                     ligase EgtA"
                     /protein_id="HEX2290998.1"
                     /translation="MTPAVPLPWASTPAAPPTVLRNREEAEVYVASVCFKHGPPKLLG
                     AELEWVVQHSGNPHRPLQARHLADALGPHVPHSLVSDSPQLPLPRGSTVTVEPGGQLE
                     ISSPPRASLTELIATVECDTAALTDLLDPAGLVLTGSGSDPHRLSRRLIDIPRYTAME
                     TAFDRIGPMGRAMMCSTAAVQVCLDAGTPTEVASRWAALHDLGPVLLAAFANSPWLGG
                     VRTGWASSRMQAWFALDPVRTMPSPARWDPAADWARRALDTPVLCQRRPWGTWDVPNG
                     VTFADWLAGALPGRPSTADLDYHLTTLFPPVRPQGYLEVRYLDTQPVGQWLAPVAVLA
                     ALLANPAVTAAARDACAPAVGLWMEAAERGLRDDALATAAVAVFELACHALPAPDAPA
                     EVQQLVEQITEGRVSRGRCPADDADDMVEQDAGRRNGIRPRALNGGQPL"
BASE COUNT          484 a         1046 c         1092 g          467 t
ORIGIN      
        1 cgggcgtggc cgaacgcacg gcagcgagca ctgcgctgct gcgcacctcg cgcgccagcg
       61 tggcgaacga cggatccgac cacatcggga ctgcccgccg gtacctgatc ggcgtggttg
      121 caccgtcggg ataccagccg gcaccgaacc cgtcgacgtt gatcgtgcca ccgcctcgca
      181 tgtcgaccgg cgcgtaggtt tgccgcagca acgagtgcgg cggatccagc agcagcgtcg
      241 caaggtcacg cggcggcccg aggtaggcca ggtgtcggca cacggtcagg ccagctcgcc
      301 ggggtgagcg tttcgcgcgc accggaaacc ggtgaagatc tgcctgcgga tcgggtggtc
      361 ccagttgcgg aaggtagccc ggcaggttgc cgcatcggtg ccgaacgagc cgccgcgcag
      421 catccggtag tcaccgccca ggaacacctc ggagtattcc cggtacggga acggtgcaaa
      481 gcctgggtag ccggagaagc ccgaggacgt ccactcccac acgtcgccca tcagctggtg
      541 cacgcccagt ggggacgccc cggccgggta gctgcccgcg agtgcgggtt gcagatgccg
      601 ctgccccagg ttggcgtgtg ctggggtggg gtcctcatcg ccccagggga agcggcggga
      661 acgtccgctg gccgggtcga atcgggcggc cttctcccac tccacctcgg tgggcaggcg
      721 cttgccggcc cacgctgcgt atgcctgcgc ctcgaaaaaa ctgacgtgca gcaccggctc
      781 gtcgggtggc acaggttcgg tgaggccgaa ccgggtgcgc caccacgacc cgccgtcgaa
      841 gtaccagaat tgcggggcta ccaggtcggc agcgcagcgg tgtgcccagc ctcgctcact
      901 ccaccaccgc gggtcgtcgt agccgcggtc ggcgagaaac cgctggtact cgccgttggt
      961 caccggagtg gtgtccaacc agtacgcgtc ggtgtaggcc cgatgtgctg gacgctcatt
     1021 atccagagcc cacggatcgg tggaggttcc catggtgaac tcgccggcgg ggatcaagac
     1081 ctcggcgggc aggtcggtgg cccgctgcgg tgccggcgcg ggggcgtgca gggcagccgg
     1141 accgactcga agctggtggg tggccagcat ggtctcgtcg tgctgctgct cgtgttgcac
     1201 gatcatcccg aaggcgaacc cgtgcgtcac cagtctgctg ccttccagcg gggtgcgctc
     1261 taaggcgtcg agcaccttgt ggcggacctc accgaggtaa cccctggcct ctaccggtgt
     1321 gagcagtggc agagcggacc ggcccgacct tggttgcttg aacgcatcgt aaaggtggtc
     1381 gatgtctgcc cggacgggct cacgcccggc cacatctcgt accagccaca gctcctcctg
     1441 gttgccgacg tgagccaggt cccacaccag cggggacatg atcttggagt gctggcggat
     1501 caggtcgccg tcgtccaccg catcagtcag cgccgtgctg cgggcccggg tgcgttcgag
     1561 ctcggctgcg aggtggtcgc gcagagcggc ggtgctcagc tcgatgatcg acgtgctcac
     1621 agcggttgtc ctccgttgag tgctcggggt cgaattccgt tacgacggcc ggcgtcctgc
     1681 tcgaccatgt cgtcagcgtc gtctgctggg cagcggccgc ggctaacccg tccttcggtg
     1741 atctgctcca cgagctgttg cacctccgcc ggcgcatcgg gcgccggcag ggcatggcag
     1801 gccagctcga acaccgcaac cgcggccgtg gcgagcgcgt cgtcgcgcag gccgcgttcg
     1861 gccgcttcca tccagagccc cacagctgga gcgcaggcgt cccgggcggc cgcggtgacc
     1921 gcagggttgg ccagcagggc cgccaacacg gcaaccggcg ccagccactg ccccaccggc
     1981 tgagtgtcca ggtagcgaac ttccaagtac ccctgcggtc gcaccggcgg gaacagcgtg
     2041 gtcaggtggt agtcgagatc ggcggtgctg ggccgaccgg gcaatgcgcc agccagccag
     2101 tcggcgaagg tcaccccgtt gggcacatcc caggtccccc aggggcgacg ctggcacagc
     2161 accggggtgt ccagtgcccg gcgggcccag tcagcggctg gatcccagcg cgccgggctg
     2221 ggcatggtac gtaccgggtc caacgcgaac catgcctgca tccgggaaga cgcccagccc
     2281 gtgcgtacac cacccagcca cggcgagttc gcgaacgcgg cgagcagtac cggtcccaga
     2341 tcgtgtagcg ccgcccagcg cgacgccacc tcggtggggg taccggcgtc cagacacacc
     2401 tggaccgcgg cggtggaaca catcatggcg cgccccatcg gtccgatccg gtcaaaggcg
     2461 gtttccatcg cggtatagcg cgggatgtcg atcagccgac gagacaggcg atgtggatca
     2521 gagcccgaac cggtgagtac cagcccggcg ggatcgagta ggtcggtcag tgcggcggtg
     2581 tcgcactcca cggtggcgat gagctcggtc agcgaagcgc ggggcgggct ggagatctcg
     2641 agctgaccac cgggttcgac cgtgacggtg gagccgcggg gaagagggag ctgagggcta
     2701 tcggagacga gagaatgcgg gacgtgcggg ccgagcgcgt cggcgaggtg gcgggcctgc
     2761 aatgggcgat gggggttccc ggagtgctgc accacccact ccagctcggc gccgagcagc
     2821 ttcggtggtc cgtgtttgaa gcacaccgag gcgacgtata cctcggcctc ttcccgattg
     2881 cgcagcaccg tcggtggtgc cgccggtgtc gatgcccagg gcagcggtac cgctggtgtc
     2941 acgacctcac ctcccggcaa aaccgacccg atcgaggagt cgactcgcca cgctacccag
     3001 ttgccccgac gtccgcagcg ttacagcagt gttacgacac ccctccgcgc gtcccccaac
     3061 ttccccccct ccggcgtgtc ctcctcccg
//
LOCUS       DATADN010000973         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_428652, whole genome shotgun sequence.
ACCESSION   DATADN010000973 DATADN010000000
VERSION     DATADN010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428652"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VHH53_12580"
     CDS             complement(<1..1012)
                     /locus_tag="VHH53_12580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005446032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2290999.1"
                     /translation="MRIGMVCPYSIATRGGVQAHVLGLASGLRGLGHQVEVLAPGAAD
                     DPVPEFVTTAGRAVGVPYNGSIAPLAFGPVAATRVRRWLRDHDFDVLHLHEPTAPSLS
                     LIALALADRPVVVTFHIATARSWALAAAHGLLQPPMEKITARIAVSPLARRLQVKYLG
                     GDAVEIPNGVDVAQLAAARPLRGYPRSGGTVGFVGRYGEPRKGMPVLLAALRTLARTR
                     PGMQLLVVGDGDDSALLRAAGPALASRIRVLGGLGDEGKAAVLASVDLLCAPALGGES
                     FGIVLIEAMAAGTPVVASDLDAFRLVLDGGQAGVLVPAGVPAALSGAIGDLLDDPGRR
                     ADL"
     gene            complement(1021..1914)
                     /locus_tag="VHH53_12585"
     CDS             complement(1021..1914)
                     /locus_tag="VHH53_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEX2291000.1"
                     /translation="MSRLAELGYAAGWRLARVAPGDVTTRVFRAGADLAARRDGVGAR
                     QLRRNLARVVPDATSVGLDALVRDGLRSYARYWQETFTLPGADHDALLERLGGAMEGV
                     DHLNRALARGRGVVVVLPHSGNWDMAGLWFVRHHGEFSTVAERLQPESLYQRFVDYRA
                     SLGFDIVPLTGAGNPMPRLLRRLRTGGVVCLLADRDLTGAGATVDFFGGAARMPLGPA
                     RLAAITGATLLVIGCWFTPDGWGIRFHPPVPVRKRSAAEATQAMADCFATDIAAHPQD
                     WHMLQPLWIDDLPVAALRDAG"
BASE COUNT          328 a          712 c          636 g          240 t
ORIGIN      
        1 ccagatcggc gcgccggccc ggatcgtcga gcaagtcacc aatcgccccg gacagcgccg
       61 ccggtacccc ggcggggacg agcacgccgg cctgcccacc atcgagcacc agacggaagg
      121 cgtcgaggtc gctggccact accggagtgc cggcagccat cgcctcgatc agcacgatgc
      181 cgaaactctc ccctccgagg gcgggcgcgc acagcaggtc cacgcttgcc agcaccgccg
      241 ccttgccctc atctcccagc ccgcccagca cccggatccg ggatgccagc gcggggccgg
      301 cagcgcgcag cagtgccgag tcgtccccgt caccgactac cagcagttgc atacctggcc
      361 gggtccgagc cagcgtgcgt agcgccgcca gcagtaccgg catgcccttg cgtggctcgc
      421 cgtagcggcc cacgaagccc accgtgccgc cggagcgcgg ataacctcgc aggggccgcg
      481 cggcggccag ctgagccacg tcgacaccgt tagggatctc cacggcgtca cccccgaggt
      541 acttcacctg tagtcgtcga gccagggggg acaccgcgat ccgggcggtg atcttctcca
      601 tcggaggctg cagcaggccg tgcgccgccg ccagcgccca cgatcgcgcc gtggcgatgt
      661 gaaaggtcac cacgacaggc cggtcggcga gtgccagggc gatcaacgac aggcttggcg
      721 cggtgggctc gtgcaagtgc agcacgtcaa agtcatggtc gcgcagccag cgccggaccc
      781 gggtggcggc gaccggtccg aacgccagcg gggcgatgga gccgttgtag ggcacgccca
      841 cggcacgccc tgcggtggtg acaaactccg gcaccgggtc gtctgccgcg cccggggcaa
      901 gcacctccac ctggtggccg aggccacgca agccgctggc taggcccagt acgtgcgcct
      961 gcacccctcc ccgggtggcg atggagtacg ggcacaccat cccgatccgc acaggcccca
     1021 ctatcccgcg tcgcgcagcg cagcaaccgg caggtcgtcg atccacagcg gctgcagcat
     1081 gtgccagtcc tgcggatgcg ccgcgatgtc ggtggcaaag caatccgcca tcgcctgggt
     1141 cgcctccgcc gctgatcgtt tccgcacggg caccggggga tggaagcgaa tgccccagcc
     1201 gtcgggagtg aaccagcagc cgatgaccag caaggtggcg ccggtgatcg ccgccagccg
     1261 agctggcccc agcggcatcc gcgcggcacc gccgaagaag tccacggtag ccccggcacc
     1321 ggtgaggtcc cggtcggcga gcaggcacac cactccaccc gtacgcagcc tgcgcagcag
     1381 gcgtggcatc ggattccccg ccccggtcag cgggacgatg tcgaaaccga gtgaggcgcg
     1441 atagtcgacg aaacgctggt acagcgactc cggctgcagc cgctcggcaa cggtgctgaa
     1501 ctcaccgtgg tgcctgacga accacaagcc ggccatatcc cagttgccgc tgtgtggcag
     1561 cactactact accccgcgac ctcgtgccag tgcgcggttc agatggtcga ctccctccat
     1621 cgcgccaccc agccgctcca acagggcgtc atggtcggca ccgggcagtg tgaaggtctc
     1681 ttgccagtac cgggcatagg agcgcaatcc gtcccgcacc agcgcgtcaa gtccgacgga
     1741 ggtggcgtcg ggaacgaccc gggcgaggtt gcggcgcagc tggcgggcac cgacgccgtc
     1801 gcggcgtgcg gccaaatccg caccggctcg aaatacccga gtagtcacgt cacccggcgc
     1861 gacccgagcc aaccgccaac ccgctgcata ccctagttcg gcgaggcggc tcacct
//
LOCUS       DATADN010000974         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_103138, whole genome shotgun sequence.
ACCESSION   DATADN010000974 DATADN010000000
VERSION     DATADN010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103138"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /gene="gmk"
                     /locus_tag="VHH53_12590"
     CDS             complement(<1..557)
                     /gene="gmk"
                     /locus_tag="VHH53_12590"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX2291001.1"
                     /translation="MTAGESGTSEVAGPRLTVVSGPSGVGKSSVGAEVLRRWPQLFVS
                     VSVTTRSPRGGQRPDEQYHYVDRATFDRMAERGEFLEYAEYAGNFYGTPAAPVHDALA
                     AGRPTLLEIEVQGARQVRAAMPDALLVMLMPPSWEALRERLAGRGTEDPAGLERRLQV
                     AREELDAVGEFDATVVNDDVGRAAQ"
     gene            complement(611..928)
                     /gene="mihF"
                     /locus_tag="VHH53_12595"
     CDS             complement(611..928)
                     /gene="mihF"
                     /locus_tag="VHH53_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEX2291002.1"
                     /translation="MALPELTEEQRAAALEKAAAARRARAELKERLKRGGTNLTEVLA
                     QSDSDDILGKMKVSALLEALPGVGKVRAQQIMERLEIANSRRLRGLGERQRKALLSEF
                     GPE"
     gene            complement(1007..>1749)
                     /gene="pyrF"
                     /locus_tag="VHH53_12600"
     CDS             complement(1007..>1749)
                     /gene="pyrF"
                     /locus_tag="VHH53_12600"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX2291003.1"
                     /translation="LERFARRAVEALAPEVAVIKPQSAFFERYGARGVAVLERVLVAA
                     RDAGALALLDVKRGDIGSTMAGYAAAYLSDGAPLAADAITVSPYLGVGALEPAFELAS
                     GTGRGVFVLALTSNPEGAAVQAALTSAGSTVGQSVIDAVTARNIGPFGDIGVVVGATT
                     PAGALRLEAFNGPILAPGIGAQGARLADLRQVFGAAFRHVLPATSRELLRHGPELASL
                     RHAARRMAGEAAELLGLPSRQVGGGTPR"
BASE COUNT          266 a          655 c          555 g          273 t
ORIGIN      
        1 tcctgcgcgg cccgccccac gtcgtcgttg acaaccgtcg catcgaactc tccgacggcg
       61 tccagttctt cccgggccac ctgcagccgg cgctccaggc cggctggatc ctccgtgcca
      121 cgaccggcca gccgctcccg aagcgcctcc caggacggcg gcatcagcat caccagcaac
      181 gcatcaggca tcgcggcccg aacctgccgg gcgccctgaa cttcgatctc cagtagcgtt
      241 ggccggcccg cggccagtgc atcgtgtact ggtgcggcgg gagtgccgta gaagttaccc
      301 gcgtactcgg cgtactccag aaactcacca cgctcggcca tgcgatcgaa ggtggcgcgg
      361 tccacgtagt ggtactgctc atccgggcgt tggccgccac gaggcgagcg ggtggtcacc
      421 gagacactga cgaacagctg cggccagcgc cgcagcacct cggcgcccac gctggacttg
      481 ccaaccccgg aagggcctga gacaacggtg agccggggcc cggcaacctc gctggtcccc
      541 gactcacccg ccgtcactac gtggtcgctc tgtgtcgact gtgctggagt tcctggttgc
      601 gcttggtcgg ttactcgggg ccgaactcgc tgagcagcgc cttacgctgg cgctcaccaa
      661 gcccacgcaa ccgccggctg ttggcgattt ccagccgctc catgatctgc tgagcgcgga
      721 ctttgcccac gccaggcagg gcctcaagca aagccgaaac cttcatcttc cccaggatgt
      781 cgtcgctgtc ggactgagcg agcacctccg tgaggttggt cccaccgcgc ttgaggcgtt
      841 ccttgagctc ggccctggcg cgtcgggcgg cggccgcctt ctccagcgca gcggcccgct
      901 gctcctcggt cagctcggga agggccacgg tctcctccgt cgattgctag tgcttaggga
      961 tggtctggat tggtgcggcg accgtatccc ccaggcggtc caccgttcaa cgcggggtgc
     1021 ccccgccgac ctgccgtgac ggcaatccga gcagctctgc cgcctcgccg gccatccgcc
     1081 gtgcggcgtg tcgcagggag gccagctcgg gaccatgccg taacagctca cgtgacgtgg
     1141 ccggcagaac gtgtctgaac gccgcaccga acacctggcg caggtcagcc aggcgggcac
     1201 cctgcgcgcc tatccctggg gccagtatcg ggccgttgaa tgcctccaac cgcagcgccc
     1261 cggcaggggt ggtggccccc acgaccactc cgatgtcgcc aaacggaccg atattgcgtg
     1321 cagtgacagc gtcgatcacc gattgcccga ccgtgcttcc cgcgctggtc aacgcagcct
     1381 gcacggcggc gccttccggg ttggacgtca gcgccagcac gaacactccc cttccggtac
     1441 cgctggccag ctcgaacgcc ggctccagcg caccgacccc gaggtagggc gacacggtga
     1501 tcgcgtcggc ggccagcggc gccccgtcgc tcaggtaggc agcggcgtat ccggccatgg
     1561 tggaaccgat gtctccccgc ttgacgtcga gcagggccag cgcgccggca tcccgggccg
     1621 ctaccaggac tcgctccagc acggcgacgc ctcgggcccc gtagcgctcg aaaaacgccg
     1681 actgtggctt gatgaccgcc acctccggcg ccagcgcctc gaccgccctc cgagcgaacc
     1741 tctcgagcc
//
LOCUS       DATADN010000975         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1481326, whole genome shotgun sequence.
ACCESSION   DATADN010000975 DATADN010000000
VERSION     DATADN010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481326"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VHH53_12605"
     CDS             complement(<1..1070)
                     /locus_tag="VHH53_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2291004.1"
                     /translation="MTAVPTPPAIRSELFGPSRRATTTGLVLLISLVAFEAMGVGTAM
                     PALVADLGAIPLYAWPFVSFMAAAVFGTVLGGRWSDRAGPGVPLVTSPLLFGIGLLVA
                     GTAGSMAQLLAGRVLQGLGAGALTVAGYVLIAVIYPERARPAVFGLLASAWVLPSLIG
                     PPAAGLVTEALSWHWVFLGLVPVVVLAVSLVVPAVRQLDPLQHDPASAPPIHGSVTAA
                     LAAAASASALSWASQRITMLAAAVAGIAVVVLVPALHRLLPSGVFRTRRGIPALVASR
                     GLLAGVFFTVNSYLPLTLNGTHGWSLAAAGIPLIVGSLGWSASAAWQGRHPELCRPRL
                     LRLGFCALAVGTAGMLLVTPSWG"
     gene            complement(1350..>1764)
                     /locus_tag="VHH53_12610"
     CDS             complement(1350..>1764)
                     /locus_tag="VHH53_12610"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013231059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX2291005.1"
                     /translation="ADLVLSLHTDGHPSTLAHGVATFHFGTGNGTTSTVGEALAAFIQ
                     RELAARTGMLDCGTHPKTWELLRMTRMPTVRIELGYLTNPEDRQKLLDPAFRDVAAEG
                     VLVAVKRLYLLGENDQPTGTFTFADLLRGELARAE"
BASE COUNT          307 a          600 c          631 g          226 t
ORIGIN      
        1 ccccacgacg gtgtgacgag cagcatcccg gccgtgccga ccgcgagtgc gcagaatccg
       61 aggcggagca gcctcggcct gcagagctcg ggatggcggc cctgccacgc ggctgacgcc
      121 gaccagccca gcgagccgac gatgagcggg atgcccgccg cggccagcga ccagccgtgg
      181 gtgccgttca gcgtcagcgg aaggtaggag ttgacggtga agaacacccc ggccagcagg
      241 ccgcggctgg ccacgagagc gggaatgccg cgccgcgtgc ggaagacccc ggacggaagt
      301 aaccggtgca gcgccgggac cagtacgacg acggctatcc cggccacggc cgcggcgagc
      361 atggtgattc gctgggatgc ccagctcagc gccgacgcgc tggcggccgc agcgagagcg
      421 gctgtcaccg atccgtgtat cggtggtgcg gaggccgggt catgctgcag tgggtccagt
      481 tgccggacgg cgggcaccac gaggctgacg gccagcacca cgaccggcac caaccctagg
      541 aagacccagt gccacgacag cgcctcggtg acaagcccgg cggccggggg accgatcagc
      601 gacggcagca cccacgccga ggcgagcaac ccaaacacgg cggggcgggc gcgctccggg
      661 tagatcacgg cgatcaagac gtagcctgcc acggtcagtg ccccagcgcc gagaccctga
      721 agcacgcggc cggccagtag ttgggccatg ctgcctgcgg tgcccgcaac gagcaggccg
      781 atcccgaaca gcaacggcga ggtgaccagc ggcactcccg gcccggcccg gtcagaccag
      841 cgcccgccga gcaccgtgcc gaacaccgcg gccgccatga acgacacgaa cggccaggcg
      901 tacagcggga tcgcaccgag gtcggcgacc agcgcgggca tcgccgtccc gacacccatc
      961 gcctcgaacg cgaccaggct gatcagtagc acgagcccgg tcgtcgtcgc tcgacggctc
     1021 ggtccgaaca gctcggagcg gatggcgggg ggcgtgggaa cggcagtcac cagcctgatg
     1081 gtgcaacctc aacccacgtg gaggtcaacc gtctggtgcc aagcacttgt tcccattgcg
     1141 tacggcagct gtcagcgcac ggtgtagggg ccggtgttga cggcgtggta ggccttggcg
     1201 ccgacggcgc gctggacgcg gacgagcgct tcactgagat gtccggaact accggtcacg
     1261 aggatccgct cgccagctac ccatccgacg gtaggtgttc cgcctggcgg caggctcact
     1321 atggtcgcgc ctgtggtggt tcgccgcggt tactccgccc gggccagctc tccgcgcagc
     1381 agatcggcga aggtgaacgt gccggtgggc tggtcgttct cacccaacag gtagagtcgc
     1441 ttcaccgcga cgagcacgcc ttcggcggcc acgtcccgga acgccggatc caggagtttt
     1501 tgacggtcct cggggttggt caggtagccc agctcgatgc gcaccgtggg catccgggtc
     1561 atccgcagca gttcccaggt tttggggtgg gtgccgcagt caagcatccc ggtgcgggcc
     1621 gccagctcgc gctgaatgaa cgcggctaag gcctcgccca ctgtcgacgt cgtgccgttg
     1681 ccggtcccga agtgaaacgt cgccacgcca tgagccagcg tcgaggggtg gccgtcggta
     1741 tgcagggaaa gcaccaggtc ggcg
//
LOCUS       DATADN010000976         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_103299, whole genome shotgun sequence.
ACCESSION   DATADN010000976 DATADN010000000
VERSION     DATADN010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103299"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1525
                     /locus_tag="VHH53_12615"
     CDS             <1..>1525
                     /locus_tag="VHH53_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX2291006.1"
                     /translation="AHFITSRICGICGDNHATCSVYCQNMAYDVRPPHLGEWIVNLGE
                     AAEYIFDHNIFQENLVGVDYCEKMVAETNPGVLDLANRTECRNAEAHGYKTIGDIMRA
                     LNPLAGEFYREALQVSRYTREMFCLMEGRHVHPSTLYPGGIGCIATVQLFTDYYTRLM
                     RYVEFMKRVVPLHEDLFQFFYDAMPGYEEVGRRRVLLGCWGALNDPEHCDFSYQNMTN
                     WGRKMYVTPGVVVDGKLVTNDLVKINLGLRILLGSSYYEDWAGKEVFVDKDPLGNPVD
                     VRHPWNQHTIPKPAKRDFNGAYSWTMSPRWFDGQDHLALDTGGGPIARLWATALAGLV
                     DTGTVKATGKSVVINLPQTMSKPAATFEWKIPYDKAGKPLSNAIERNRARTYFQAYAA
                     GIALHQIEKALAEVRAGRTKTWEPFKVPKEAVSCGWTEAVRGVLSHHMVIQDGKIANY
                     HPYPPTPWNGSVRDVYGTPGPYEDAVQNTPIFEENPPENFKGIDIMRAVRSFDPCLPC
                     "
BASE COUNT          296 a          517 c          471 g          241 t
ORIGIN      
        1 cgcccacttc atcaccagcc gcatctgcgg catctgcggt gacaaccacg ccacctgttc
       61 ggtgtactgc cagaacatgg cctacgacgt ccgcccgccc cacctgggcg aatggatcgt
      121 caacctcggc gaggcggccg agtacatctt cgaccacaac atcttccagg agaacctcgt
      181 cggggtcgac tactgcgaga agatggtggc cgagacgaac cccggcgtcc tcgacctggc
      241 caaccgcacg gagtgccgca acgccgaggc gcacggctac aagaccatcg gcgacatcat
      301 gcgggccctc aacccgctgg cgggtgagtt ctatcgcgag gcactgcagg tctcccgcta
      361 cacgcgagag atgttctgcc tcatggaggg ccgccacgtg cacccctcca cgctctaccc
      421 cggtggcatc ggctgcatcg ccaccgttca gctgttcacc gactactaca cccgcctgat
      481 gcggtacgtg gagttcatga agcgggtcgt gcccctgcac gaggacctct tccaattctt
      541 ctacgacgca atgcctggct acgaagaagt cggccggcgc cgggtgctgc tgggttgctg
      601 gggcgccctc aacgacccgg agcactgcga cttcagctac cagaacatga ccaactgggg
      661 ccgcaagatg tacgtcaccc ccggtgtggt ggtggacggc aagctggtca ccaacgatct
      721 cgtgaagatc aacctcgggt tgcggatcct gctcggctcc tcgtactacg aggactgggc
      781 cggcaaggag gtcttcgtcg acaaggaccc gctggggaac ccggtcgacg tgcggcaccc
      841 gtggaaccag cacaccatcc cgaagccggc caagcgcgac ttcaacggtg cctacagctg
      901 gaccatgtcg ccgcggtggt tcgacggcca ggaccacctc gccctggaca ccggtggcgg
      961 gcccatcgcc cggctgtggg cgaccgcatt ggccggcctg gtggacaccg gcactgtcaa
     1021 ggccaccggt aagagcgtgg tgatcaacct gccgcagacg atgtcgaagc cggcggccac
     1081 gttcgagtgg aagatcccct acgacaaggc cggcaagccg ctgtccaacg cgattgagcg
     1141 caaccgggca cggacctact tccaggccta tgccgcgggt attgcgctgc accagataga
     1201 gaaggccctg gctgaggtgc gcgctggtcg caccaagacc tgggagccgt tcaaggtgcc
     1261 caaggaagcg gtcagctgtg gatggaccga ggcggtccgt ggtgtgctgt cgcaccacat
     1321 ggtgattcag gacgggaaga tcgccaacta ccacccgtac ccacccaccc cgtggaacgg
     1381 cagtgtccgc gatgtctacg gaactccggg accctacgaa gacgccgtgc agaacacgcc
     1441 gatcttcgag gagaaccccc cggagaactt caagggcatc gacatcatgc gggcggtgcg
     1501 cagcttcgac ccgtgcctgc cctgc
//
LOCUS       DATADN010000977         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_428956, whole genome shotgun sequence.
ACCESSION   DATADN010000977 DATADN010000000
VERSION     DATADN010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428956"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..916)
                     /locus_tag="VHH53_12620"
     CDS             complement(8..916)
                     /locus_tag="VHH53_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2291007.1"
                     /translation="MDDAGSAIARVVTLPGRGSTVVWDCPGPPGAPTLVLLHGVTLTA
                     ELNWSAVFPVLGRHFRVLAFDQRGHGSGLGCVGGYRLEQCADDVAALAAVLGIERFIA
                     VGYSMGGLIAQLCWRRHPELTAGLVLCASARSIAGSPWARWVTLMMPAMMMPAVAAAA
                     GSMPGMDGLRADVIGGQMLGQDGDRAGRAWALDQMRRTPLLTALSAVSAVYAFDSHDW
                     IGGVDVPTAVIITRHDRVVSAARQRRLAAAVPGALAYDIDGDHGVFLTAPGRFTATLL
                     AACEAICSDDTEITGPTDPAAGLTAS"
     gene            complement(1069..1539)
                     /locus_tag="VHH53_12625"
     CDS             complement(1069..1539)
                     /locus_tag="VHH53_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291008.1"
                     /translation="MTQAKETDFASYARLSESEREVLEQALASIPEEILSTGDRASIE
                     SRIRNRLQREGLLGTGEAGGPLGVAEIPAVAFVGRAVGCLASGYVSLRGISHNKPADT
                     VADSIARAVADCVPGDIDAIKSDILTYRSQVAGALTALGLPALGDALLANHPAD"
BASE COUNT          329 a          655 c          543 g          257 t
ORIGIN      
        1 ggcagggtta agaggcggtg agtccggcag cggggtcggt cggaccggtg atctccgtgt
       61 cgtcagaaca gatcgcctcg caggcggcga gcagcgtagc ggtgaagcgg cccggagcgg
      121 tgaggaagac accgtgatcg ccgtcgatgt cgtaggccag tgccccaggc accgcggcgg
      181 ctagcctccg ctgccgggcc gccgacacca cccggtcgtg ccgggtgatg atcacagcgg
      241 tgggaacgtc gaccccgccg atccagtcgt gggaatcgaa tgcatagacc gccgagaccg
      301 cagacaacgc agtgagcaat ggggtgcggc gcatctgatc cagcgcccat gcccggccag
      361 cccgatcacc gtcctgaccg agcatctggc cgccgatgac gtcagcccgc aacccgtcca
      421 tcccgggcat cgatcccgcc gcggccgcca ccgccggcat catcatcgcc ggcatcatca
      481 gcgtcaccca acgtgcccac ggcgagccgg cgatgctgcg ggcactcgcg cacaacacca
      541 gcccggcggt caactccgga tgccggcgcc agcacagttg cgcgatcagc ccgcccatcg
      601 aatagccgac cgcgatgaac cgctcgatgc ccagcaccgc ggccagcgcg gcgacatcat
      661 cggcacactg ctccagccgg tagcctccca cgcagcccag cccactccca tgcccacgct
      721 ggtcgaaggc cagtacccgg aagtgccggc ccagcaccgg aaacaccgcc gaccaattga
      781 gctcggcggt gagggtgaca ccgtgcaaga gcaccagagt gggcgccccc ggcgggccag
      841 ggcagtccca gaccaccgtg ctgccccgcc caggcagcgt caccacccgt gcgatcgccg
      901 agcccgcgtc atccatcgac tgccgccctt cgcaacggcg gaccccgcca gcggcgctgc
      961 cggcgttctc ggccctgacc ataacccgta aaccgccgga tgacatagcg tcacatgtca
     1021 gtggtctgcc gttggcttga ggacgccacg ggccggaagg ccgcatcgtc aatctgctgg
     1081 atgattcgca aggagcgcgt cgcccagcgc aggaagcccc agcgccgtga gagcgcccgc
     1141 gacctggctc cggtatgtga gaatgtcaga tttgatcgca tcgatatcgc ccgggacgca
     1201 gtccgcgacc gcccgtgcga tcgagtcagc taccgtgtcg gcaggcttat tatgacttat
     1261 tccccgcaag gacacatagc ctgacgcaag gcaccccact gctcgcccca cgaacgccac
     1321 cgcaggaatc tccgcgacac ctaatggccc cccggcctca cccgtgccga gaagcccctc
     1381 ccgctgcagg cggttgcgga tccgcgactc gatcgacgcg cgatcacccg tcgataagat
     1441 ttcttcaggg atagacgcga gtgcctgctc gagcacttcc cgctccgact ccgacaatcg
     1501 agcatacgaa gcaaagtccg tctcttttgc ttgagtcacc gccacctccg tgtttgacag
     1561 ccctgagccc agtcagctta gcgtacgagg taccggtgtc aggcgactcg cgcgaccagc
     1621 ttcgcgcggt gagaaaagca ccaatacacc gatctcttac gcgcatgggc acggtgggcc
     1681 gcacgtccat tgtcgtctcc cgggcatctc ccgccagcgc ccgaaacatc gtggccggca
     1741 tgaggcgtac ggctgctctg ggctcctctg ggcccctgca cggg
//
LOCUS       DATADN010000978         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1182193, whole genome shotgun sequence.
ACCESSION   DATADN010000978 DATADN010000000
VERSION     DATADN010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182193"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1751
                     /locus_tag="VHH53_12630"
     CDS             258..1751
                     /locus_tag="VHH53_12630"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2291009.1"
                     /translation="MRPDHVDVLIIGAGLSGIGAACHLRQRCPSKTFAILEARGEIGG
                     TWDLFRYPGVRSDSDMFTLSYSFRPWTGAKAIVDGDSICRYIRETAREHGMDKHIRFQ
                     HRVVRAEWSSEDARWTVEARRTDTGESVVLTCSFLFGCSGYYRYDEGYTPQFPGIDRF
                     TGQVVHPQQWPGDLDYAGKRVVVIGSGATAATLVPAMTDKAAHVTMLQRSPSYVLSLP
                     ATDAVADALRRRLPPKVAYPLIRWKNALLATLTFQLSRRAPGVIKALLRKGAAGQLPE
                     GYDVGTHFTPQYNPWDQRLCLVPDGDLFKAIRSGRASVVTDHIETFTEHGIRLASGTE
                     LEADIVITATGLNLLLIGGMALSVDGADIDLGRTVAYKGLMLSGVPNFALTIGYTHAS
                     WTLKGELVSEYVCRLLNHMDQRGYQVCTPLAPDTPQREPLIDLQAGYVLRSVAALPKQ
                     GPAAPWRQYQNYPRDVLLLRRGPLEDEGVRFTRAPAAVRAAGTAEMA"
     gene            1812..2252
                     /locus_tag="VHH53_12635"
     CDS             1812..2252
                     /locus_tag="VHH53_12635"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2291010.1"
                     /translation="MGPPVVLLHGIGRSIEDWTEQHELLRGQHRVYSVDLPGHGKSEP
                     LAEPYTLAALARFVADFLDVLDITEPAHLVGHSLGGAVATQLAVHAPDRVASLALVTA
                     PALDARSRSRCGCSRSVHWAGSCCGRVASAHAGWSAACSTIRRS"
     gene            2249..2605
                     /locus_tag="VHH53_12640"
     CDS             2249..2605
                     /locus_tag="VHH53_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291011.1"
                     /translation="MTEERVEHTFTLAQRPHAARVLLETVRGLGTFPRCPPAMAPDPA
                     ARRRRPGIPTLVVWGDRNLILPAAHLNAARARLPHVPTYSPDTGHMPQIERAEAFSHL
                     VGHFWRKAGWFPDKGR"
BASE COUNT          426 a          910 c          860 g          464 t
ORIGIN      
        1 tcgatggaga tctcgctcag cgtccgatcc tccagcagcc gctctgccgt ggcgaggatt
       61 gctatctgcc gctcgtcgcc agatggtcga ggcgtgcgcc ggcctctgga cgtaccagtg
      121 tgcaccgtgg tgaagcctgg cctcatactc ggactctaca tgtcgtcgag gctatcaaca
      181 tactgttgga atttttcgac gtgtcgtcga taccctcacg ttgaggcgcg gttcagtgcg
      241 tgaggtgagg tgaaggcatg cggccagacc acgtcgatgt gctgatcatt ggcgccggtt
      301 tgtccgggat cggcgcagcc tgccacctgc gccagcgttg cccgagcaag acgttcgcga
      361 tcctggaggc ccgcggggag atcggtggga cctgggatct gttccgctac ccaggtgtcc
      421 gctcggactc ggacatgttc accctcagct actcgtttcg cccgtggacg ggtgcaaaag
      481 ccatcgtcga cggagactcg atctgccggt acatccgtga gaccgcgcgc gagcatggca
      541 tggacaagca catccggttc cagcaccggg tggtgcgggc cgagtggtcg agcgaggacg
      601 cccgctggac cgtcgaagcg cgcaggaccg acaccggcga atcggtggtg ctgacgtgca
      661 gctttctgtt cggctgttcc ggctactacc gctacgacga gggctacacc ccccagttcc
      721 cgggcattga ccgcttcacc gggcaggtcg tgcaccctca gcagtggccc ggcgatttgg
      781 actacgccgg caagcgggtg gtcgtcatcg gcagcggcgc caccgcggcg acgctcgtcc
      841 cggcgatgac ggacaaggcc gcgcacgtga cgatgctgca gcgctcaccc agctacgtac
      901 tgtcgctacc ggcgaccgac gctgtcgccg atgcactgcg ccgcaggctg ccacccaaag
      961 tcgcctatcc cctgatccgg tggaagaacg ccctgctcgc cacgctgacc ttccagctca
     1021 gccgccgcgc ccctggcgtg atcaaggctt tgctgcgtaa gggcgccgcg ggacagctgc
     1081 ccgaaggcta cgacgtcggc actcacttca cgccccagta caacccctgg gatcagcggc
     1141 tgtgtctggt gccggatggc gatctgttca aggcgatccg tagcggccgc gcgtccgtgg
     1201 tcactgacca catcgagacc ttcaccgagc atgggatccg gctggcttcc ggaaccgagc
     1261 tggaggccga catcgtgatc accgccaccg gtctgaacct gctgctgatt ggtggcatgg
     1321 cgttgagtgt cgacggtgcc gacatcgacc tcggtcgcac cgtggcctac aagggtctga
     1381 tgctgtccgg ggtgccgaac ttcgccctga ccatcggtta cacccacgct tcgtggacgc
     1441 tgaagggtga gctggtcagc gaatacgtct gccggctgct caaccacatg gaccagcgtg
     1501 gctatcaggt gtgcacgcca ttggcgcccg acacgcccca gcgggagccg ctcattgatc
     1561 tgcaggccgg ctacgtgttg cgcagtgtgg ccgcgctgcc caaacagggg ccggctgccc
     1621 cgtggcggca gtaccagaac tacccacggg acgtgctgct gctgcggcgc ggcccactcg
     1681 aggacgaggg tgtgcggttc acccgtgcgc cggcagcggt acgcgccgcg gggaccgcgg
     1741 aaatggcctg acgtccccaa catcggcaca tcggagatgc cgagcggctc tccggtaacc
     1801 gggtggacgg tgtgggccct ccggtggtcc tgctgcatgg tatcggccgc agcatcgaag
     1861 actggactga acagcacgaa ctgctgcgtg gtcagcaccg cgtatacagc gtggacctgc
     1921 ccggccacgg caagtctgaa ccgctcgcag agccctacac cctggcggcg ctggcccgct
     1981 tcgtcgcgga cttcctcgac gtgcttgaca tcaccgaacc cgcacacctt gtcggtcact
     2041 cccttggtgg tgcagttgcc acgcagctgg ccgttcacgc acccgaccgg gtcgccagcc
     2101 tggctctggt cacagcgccg gctttggacg cgaggtcacg atcgcgctgc ggctgctcgc
     2161 gatccgtcca ctgggccggc tcctgctgcg gccgagtcgc ctcagcgcac gccgggtgga
     2221 gcgcagcttg ttccacgatc cggcgttcgt gaccgaggaa cgcgtcgagc acaccttcac
     2281 gctggctcaa cgaccgcacg cagcgagggt gttgctggag accgtccgcg gcctgggcac
     2341 attcccgcgg tgcccgcccg caatggcgcc agaccctgct gcacgccgtc gccggcctgg
     2401 catacccacc ctcgtcgtgt ggggcgaccg taacctcatc ctgcccgctg cccacctcaa
     2461 cgcagcccgc gcacgcctgc cgcacgtacc tacctactcc cccgacaccg gccacatgcc
     2521 acagatcgag cgcgccgagg cgttcagcca tctcgtcggg cacttctggc gtaaggcagg
     2581 atggttccca gacaaaggga ggtagggcat gatcgttgag gtgatgtgcc gcattgccga
     2641 tggcccgctc aggccaggcg
//
LOCUS       DATADN010000979         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1031731, whole genome shotgun sequence.
ACCESSION   DATADN010000979 DATADN010000000
VERSION     DATADN010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031731"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VHH53_12645"
     CDS             complement(<1..197)
                     /locus_tag="VHH53_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291012.1"
                     /translation="MTGSAPYKGRPGRRRRLKGAPGRGTSAKEKPPILDLLQRGGKVV
                     VRMLANVQQATIRPIIEASVA"
     gene            complement(451..1596)
                     /locus_tag="VHH53_12650"
     CDS             complement(451..1596)
                     /locus_tag="VHH53_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2291013.1"
                     /translation="MEISPTPAAAATGIQPATLFVALELSKAKWLVGLHSPAADKISR
                     HTIAGGDAPALLSLIEVARRRAEASLGGEVQVVACYEAGYDGFWLHRLLLAHGIANQV
                     VDPASLLVNRRARRRKTDRIDLAGLLRTLMAWHRGEPQVCSMVRVPSLEEEDQRRRGR
                     ERERLVKERVQHLGRIKGLLMTQGIRDFQPARRGWQERLEALRTGNGQPLPVCLKAEI
                     ARECRRLALVDEMLEELEREREAASDGKVPQQAALLGKLRGIGPISAHLLAEEVFHRD
                     FANRRQVAAYLGLEPSPWQSGQRDHEQGISKAGNRRARRVAIELAWFWLQHQPDSGLS
                     RWFKERVGTAKGRVRRIMLVALARKLIVALWRYLASGTVPEGALMRA"
BASE COUNT          267 a          668 c          606 g          309 t
ORIGIN      
        1 ttggcgacgc tggcctcgat gatcggtcgg atcgtcgcct gctggacgtt ggccagcatc
       61 cggaccacca ccttgccgcc acgttgcagc aggtccagga tcggcggctt ctctttggcc
      121 gacgtaccgc gtccgggcgc acccttcagc cgccggcgtc gccctggtcg ccccttgtat
      181 ggggcgctcc ctgtcaagaa ggatcggcag gggatgtgct tgtgctcgat gttccgagga
      241 ctgggccgct gccagcggcg ggcgagggcc cgccgaacct tgtatccggt cggctcgcat
      301 cgagccggac ccctatcccg catccagcgc ggtcgtcggc ccaggtgaac gggacccatc
      361 tttttaacgg tggatgaacc caaagggggc tcggtcacgc gtccatcctg gagcatcggg
      421 aagcgtcgct gccgctcccg atcttgtcgc tcacgcccgc atcaaggcac cttccggcac
      481 agtgccgctg gccagatagc gccacagagc gacgatcagc ttgcgtgcca gcgccaccag
      541 catgatccgg cgcacgcgac ccttggcggt accgacccgt tctttgaacc agcggctcag
      601 tccgctgtcg ggctggtgct gcagccagaa ccaggcgagc tcgatggcaa cacgccgcgc
      661 gcgtcggttg ccggccttgc tgataccctg ctcgtggtcg cgctggccac tctgccaggg
      721 gctcggctcc agaccgagat aggccgcgac ctggcggcgg ttggcgaagt cacggtggaa
      781 cacctcctcc gccagaaggt gcgccgagat cgggccgatc ccgcgcagct tgccgagcag
      841 ggctgcctgc tgtggcacct tgccgtcgct cgcggcctcg cgttcccgct cgagctcctc
      901 cagcatctcg tccaccagcg ccagcctccg gcactcgcgc gcgatctcgg ccttgaggca
      961 gaccggcaga ggctggccgt tacctgtgcg cagagcctca agacgctcct gccagccccg
     1021 ccgggcaggc tggaagtcac ggatgccctg ggtcatcagc agccccttga tccggcccag
     1081 atgctggacg cgttctttga ccagccgctc ccgctcccgg ccgcggcgcc tctgatcctc
     1141 ctcctcgagg ctgggcacgc ggaccatgct gcaaacctgc ggctcaccgc gatgccatgc
     1201 catcagcgtg cgcagcaggc cggcaagatc aatccgatcg gtcttgcgtc ggcgggcacg
     1261 gcggttgacc agcaggcttg ccgggtcgac aacctgattg gcgatgccgt gcgccagcag
     1321 gagccggtgc agccagaagc cgtcatagcc ggcctcatag caggcaacga cctgcacctc
     1381 gccgccaagg ctcgcttcgg cacgccggcg cgccacctcg atcaggctca gcagggccgg
     1441 cgcatcaccg ccggcgatcg tgtgccggct gatcttgtcg gcagccgggc tgtgcagacc
     1501 taccagccac ttggccttgc tgagctccag cgccacgaac agtgtggcag gctgtatgcc
     1561 ggtggcagct gctgcgggcg tgggcgagat ctccatgcga cggcctccga tggggtcaga
     1621 gcgggcactc ggaccctacc cagcccgcac acccgccgcc ccatgggatc tttttgcgtg
     1681 catcgctggc cctggaggag cagcttctcg tctcggtccg caccagcctg ccggcaccag
     1741 ctccgcagcg cgccttggcc gtgttcgggc cgcagcgggc aggcgcgcag gagatggcca
     1801 agccggcgcc cttcgtcctg ccgattgctg gcggtagggc tgtggccgac
//
LOCUS       DATADN010000980         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_103637, whole genome shotgun sequence.
ACCESSION   DATADN010000980 DATADN010000000
VERSION     DATADN010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103637"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VHH53_12655"
     CDS             complement(<1..527)
                     /locus_tag="VHH53_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX2291014.1"
                     /translation="MSTTDSEDFTDAVFADQEWESRCFVNCRFTDADLRGLRTRSCRF
                     THCDFTRADLGASEHRASAFHSCTFQRTMLAGSVLANCSLLGSTVSDCRLRPWTLHEV
                     DLSLIALGQADLRNVVLAGLRLAEANLVQADLRGADLSRADLTGARMLGARLEGADLR
                     GTRLDADALVQARLSG"
     gene            complement(634..>2172)
                     /locus_tag="VHH53_12660"
     CDS             complement(634..>2172)
                     /locus_tag="VHH53_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005447110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX2291015.1"
                     /translation="AAGLVKFDLLGLGMLSALHYAVDLVARHYRHRVQLVDLQPTDPA
                     VYEMLARADSVGVFQVESRAQMATLPRLKPRCFYDLVVEVALIRPGPIQGDSVHPYIR
                     RRNGQEAWDYDHPLLANALRKTLGVPLFQEQLMQMAIDVADFSAAEADELRRAMGAKR
                     STERMERLRERLFNGMASHGIKDELAEKIYAKLLAFANFGFPESHSISFASLVYYSAW
                     FKHYYPAAFCAALLQAQPMGFYSPQSLIADARRHGVTVRGPDVNASGVHAGLEPAAEG
                     APEQAVRLGLAMVRTVSQSLASRLVEERKTHGPYRSIADVAHRVGLTTAQAEALATGG
                     AFDRLAPYRRQALWSAGAVAAERPDRLPGTAVGVVAPTLPGMSELELAAADVWATGVS
                     PNSFPTQFLRADLAGLGVVPAAELQLRQDGQRVMVAGAVTHRQRPATAGGVTFLNLED
                     ETGMVNVICSPGFWVRYRTVVQACPALLLRGRLQIAEGVINVLAEHATALNMRVRSNS
                     RDFR"
BASE COUNT          363 a          738 c          734 g          337 t
ORIGIN      
        1 ccggacagtc gagcctggac cagggcgtcg gcgtccagcc gggtcccgcg taggtcggca
       61 ccctccagcc gagcgcccag catccgggcc ccggtcaggt cggctctgga gaggtcagcg
      121 cccctcaggt cagcctgtac cagattcgcc tcggccagcc gcagcccagc gagcaccacg
      181 ttgcgcaggt cggcttgccc cagcgcgatg agactcaggt cgacctcgtg cagcgtccac
      241 ggccgcagcc ggcagtcgct gaccgtgcta cccagcagcg agcagttcgc gagtaccgag
      301 ccggccagca tggtgcgttg gaaggtgcag ctgtggaatg cgctcgcgcg gtgctcggag
      361 gcgccgaggt cggcgcgggt gaagtcacag tgggtgaagc ggcagctgcg ggtgcgcagg
      421 ccccgcaggt ctgcgtcggt gaaccggcag ttgacgaagc agcggctttc ccactcctgg
      481 tcggcgaaca ccgcatcggt gaagtcctcg ctgtcggtcg ttgacacacc tgcaccctcc
      541 caggtcgccg cccatgatcg ctgtttgtgt gccgtgaacg acaaatcgtg tagcgcaacg
      601 cacacaaatc gtgatcaagc tgtgcgccat gcttcaccgg aagtcgcggg agttcgaacg
      661 cacccgcatg ttcagtgctg tggcgtgttc ggccagcacg ttgatcactc cctcggcgat
      721 ctgcagccgg ccgcgcagca gcagcgccgg gcaggcctgc acgacggtgc ggtagcggac
      781 ccagaagccc ggtgagcaga tcacgttcac catgccggtc tcgtcctcca agttgaggaa
      841 ggtgacccca ccggcggtgg ccgggcgctg ccggtgggtc accgcaccgg cgaccattac
      901 ccgctgcccg tcctgccgca gctgcagctc ggcggcgggc accacgccca gcccggccag
      961 gtcggcccgg aggaactggg tggggaagct gttaggcgaa accccggtgg cccacacgtc
     1021 ggctgcggcg agttccagct cactcatccc cggcagcgtg ggagcgacga cgcccaccgc
     1081 ggtgccgggc aaccgatccg gccgctcggc ggctaccgcc ccggcggacc acagcgcttg
     1141 gcggcggtac ggggcaagcc ggtcgaacgc tccgccggtg gccagtgcct cggcctgggc
     1201 ggtggtcagc cccacccggt gtgcgacatc agcgatgctg cgataggggc cgtgcgtttt
     1261 tcgctcctcg accagccggc tggccagtga ttggctcacc gtgcgcacca tggccaggcc
     1321 cagccgcacc gcctgctccg gcgcgccctc tgcggctggt tccagcccgg catgcacccc
     1381 gctggcgttg acgtccggac cccggaccgt gacgccgtgg cggcgggcgt cggcaatcag
     1441 tgactgcggg gagtagaaac ccatcggctg ggcctgcagc agggcggcgc agaacgccgc
     1501 cgggtaatag tgcttgaacc aggcgctgta gtagaccagt gaggcgaagc tgatcgagtg
     1561 gctttccggg aaaccgaaat tggcgaaagc cagcagtttg gcgtagatct tctcggcgag
     1621 ctcgtctttg atgccatgac tggccattcc gttaaatagc cgttcacgca gccgctccat
     1681 tcgctcggta gaccgtttgg ctcccatcgc acggcgcagc tcatcggcct cggcggcgga
     1741 gaagtcggcg acgtcaatgg ccatctgcat cagctgctcc tggaacagcg gcacacccag
     1801 cgttttgcgt agtgcattcg ccagcaacgg atggtcgtaa tcccaggcct cctgaccgtt
     1861 gcggcgccgg atgtagggat gcaccgagtc accctggatc gggccggggc gtatcagcgc
     1921 cacctcgacc accagatcat agaagcagcg cggtttcagc cgcggaaggg tggccatctg
     1981 cgcgcgggac tccacctgga acaccccgac cgaatcggcc cgggccagca tctcgtagac
     2041 ggccggatcg gtgggctgca aatccacgag ctgcacccgg tgccggtaat ggcgagcgac
     2101 cagatcaacc gcgtaatgca gcgcggaaag cattccgagc ccgagcaggt cgaatttgac
     2161 cagaccggcc gc
//
LOCUS       DATADN010000981         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_103736, whole genome shotgun sequence.
ACCESSION   DATADN010000981 DATADN010000000
VERSION     DATADN010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103736"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VHH53_12665"
     CDS             complement(<1..745)
                     /locus_tag="VHH53_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2291016.1"
                     /translation="MVVLAGHVDSADPATHNAQAHATMVADLRQRLATARLGGPPKTR
                     ERHVERGKLLPRERVDALLDPSSPFLELSPLAATGMYGEEAPGAGIITGVGRVAGREC
                     VIVANDATVKGGTYYPMTVKKHLRAQEVALHNHLPCLYLVDSGGAYLPEQDQVFPDRE
                     HFGRIFYNQATMSARGIPQIAAVLGSCTAGGAYLPAMSDEAVIVREQGTIFLGGPPLV
                     KAATGELLRRDDLAGGRLHQGRAAEEDRAL"
     gene            831..1484
                     /locus_tag="VHH53_12670"
     CDS             831..1484
                     /locus_tag="VHH53_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2291017.1"
                     /translation="MALPSAAGGTPTRREQILHVAATLFARHGFHGVSIAELGAAVGI
                     SGPALYRHFPGKEAVLAEMLVGISEYLLDGGRARARTDDPGQALTELVEFHVDFALQH
                     PQLITVQDRDLANLPVQVRQQVRALQRAYLQIWVDTLRELIPELSAEAARIAAHGAFG
                     LLNSTPHSAPGSDPQQVAALLRRMAMAALSDPGLASSHPSSRQVVPEDGLDGLAHRQ"
     gene            1563..1784
                     /locus_tag="VHH53_12675"
     CDS             1563..1784
                     /locus_tag="VHH53_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291018.1"
                     /translation="MHDFTTTTLAEPIPPDGLRNVAKRIDTLLVNPLDQLPAGTFAGR
                     EQNLAIDDIQRNLAFRNLVRANMVRLASG"
     gene            1900..2160
                     /locus_tag="VHH53_12680"
     CDS             1900..2160
                     /locus_tag="VHH53_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291019.1"
                     /translation="MVANTPLWFYILKEAEINGGKLGAVGGWIVAEVFHRAMEVSTHS
                     IVRKPQWRPTFCPDRDTFRMVDLRGVARLARRRSVDGGTITG"
     gene            2242..>2513
                     /locus_tag="VHH53_12685"
     CDS             2242..>2513
                     /locus_tag="VHH53_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX2291020.1"
                     /translation="MRRITGGAFERLTDHPAHALVGVLRIPEIRQGPGASIGRRIVGA
                     ALALTVTVLLVYLGRDGYRDVNEDGLSLLDCIYYATVSLSTTGYGD"
BASE COUNT          396 a          883 c          810 g          424 t
ORIGIN      
        1 ccagggcacg atcttcctcg gcggcccgcc cctggtgaag gcggccaccg gcgaggtcgt
       61 cacggcggag gagctcgccc gtggcggcct tcaccagggg cggtccgccc aggaagatcg
      121 tgccctgctc gcggacgatc accgcctcgt cgctcatcgc cggcaggtag gcgccgcctg
      181 cggtgcagga accgagcact gccgcgatct gagggatgcc gcgtgcggac atcgtcgcct
      241 ggttgtagaa gatccggccg aaatgctccc ggtcggggaa cacctggtcc tgctcgggca
      301 ggtaggcccc gccggagtcg acgaggtaga ggcagggcag gtggttgtgc agcgccacct
      361 cctgggcgcg caggtgcttc ttgaccgtca tcgggtaata ggtgccgccc ttgacggtgg
      421 cgtcgttggc gacgatcaca cattcccgcc cggctacccg cccgaccccg gtgatgatcc
      481 ccgcgcccgg ggcctcctcg ccgtacatgc cggtagccgc cagcggggac agttccagga
      541 atggcgagga cgggtccagc agcgcgtcga cgcgttcgcg cggcaacagc ttgccgcgct
      601 cgacatggcg ttctcgagtt ttcggcgggc cacccagccg ggccgtcgcg agccgctggc
      661 gcaggtcggc gaccatcgtg gcgtgcgcct gagcgttatg cgtggctggg tctgcggagt
      721 cgacgtggcc ggccagaacg accatcgtca cgccctaatc tccctgtgcc gcagttagcg
      781 ctcgttaacc acgttagcgc tcattaacat cgcccgctac ggtcttgcgc atggcactcc
      841 cttccgcagc gggcggcacg ccgacccggc gggagcagat cctgcacgtc gccgcgacac
      901 tgttcgctcg gcacgggttt cacggagtga gcatcgccga gctcggtgcc gcggtgggca
      961 tcagcgggcc cgcgctgtac cggcacttcc cgggcaagga ggcggtgctg gccgagatgc
     1021 ttgtcggcat cagcgagtac ctgctcgacg gtggacgcgc tcgagctcgc actgatgacc
     1081 ccggccaggc gctgaccgag ttggtcgagt tccacgtgga cttcgcgttg cagcatcccc
     1141 agctgatcac cgtacaggac cgtgacctgg cgaaccttcc tgtgcaggtg cggcaacagg
     1201 tccgcgccct gcagcgggct taccttcaga tctgggtgga cacgctgcgc gaactcatcc
     1261 ccgagctgtc cgccgaagct gctcgcatcg ccgcccacgg ggcgttcggg ctgctcaact
     1321 ccacgccaca cagcgccccc ggctccgatc cgcagcaggt ggccgcgctg cttcgccgga
     1381 tggcgatggc cgcgctgtcc gatcccggcc ttgccagcag ccatccgtcc agccgccaag
     1441 tggttccgga agacggcctc gatgggcttg cacaccgcca ataggtccaa cggcggaccg
     1501 actggtgtcc tcgggatcgc cagcctgttc cagctattca ccttcagcgg gaccggcgcc
     1561 ggttgcatga cttcaccact accaccctgg ccgagccgat cccgcccgac ggactccgta
     1621 acgttgccaa acgcatcgac actttgctgg tcaacccgct ggaccagctg ccggccggca
     1681 ccttcgcggg ccgcgaacaa aacctggcca tcgacgacat ccagcgcaac ctggctttcc
     1741 gcaacctcgt ccgcgcgaac atggtgcggc tcgcctccgg gtagcaaatg gcgtaccagt
     1801 tcggtgccaa gcactcaccg ccgatcgaat cctcattggt gacggcgccg gcgcaatctg
     1861 accgccccgg accaactcaa cgaggcacag caagaagccg tggtcgccaa cactccactg
     1921 tggttctaca tcctgaaaga agccgaaatc aacggcggca agctcggtgc tgtcggtggg
     1981 tggatcgtgg ccgaggtgtt ccaccgtgcg atggaagtca gcacccactc catcgtccgt
     2041 aagccccagt ggcggcccac cttctgcccg gaccgcgata ccttccggat ggtcgacttg
     2101 cgcggcgttg ctcggctcgc ccgccgacgt agtgtcgatg gcggcacaat cacaggctga
     2161 tgtgctgcga ttaggctccg actccgtgag agatgagtcg tccgagcgtg ccgcgcacct
     2221 gtggctcggc gcgctgcctt catgaggcga ataaccgggg gcgccttcga gcgcctcacc
     2281 gatcatccgg cccacgcgct ggtgggcgtc ctccggatac cggagatccg gcaaggcccg
     2341 ggagcgtcga tcgggcgccg gatcgtgggc gccgccttgg cgctcaccgt cacggtgctc
     2401 ctcgtctacc ttggccgcga cggctaccgc gatgtcaacg aagacggcct ttccttgctg
     2461 gactgcatct actacgccac agtgtcgctt tccacgaccg gatacggcga cat
//
LOCUS       DATADN010000982         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1182497, whole genome shotgun sequence.
ACCESSION   DATADN010000982 DATADN010000000
VERSION     DATADN010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182497"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..417)
                     /locus_tag="VHH53_12690"
     CDS             complement(88..417)
                     /locus_tag="VHH53_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2291021.1"
                     /translation="MTVLLRRYETAHGKWVFPSTRDSLRDPDNTRKQLRAVVRETPWQ
                     GLHPHAFRRLVATQMDVAGASAREIADYLGHERVSMRQDVYMSRGITGDAAMAALERL
                     SPPESCG"
     gene            complement(514..1644)
                     /locus_tag="VHH53_12695"
                     /pseudo
     CDS             complement(514..1644)
                     /locus_tag="VHH53_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611204.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1704..1928)
                     /locus_tag="VHH53_12700"
     CDS             complement(1704..1928)
                     /locus_tag="VHH53_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291022.1"
                     /translation="MEFLGLEWGQAAGFGDQRRGQDTDGLAIGEGPEGGGADQYFLPR
                     TGGAVKDGEQLCAVTVAVQTQRSAADQVPG"
BASE COUNT          346 a          753 c          621 g          404 t
ORIGIN      
        1 gctaggccgc aggttcgagt cctgccgggg gcgcacaccg cagatcacag gcttggctcg
       61 tgatcacatg caagatcggt ccatggttta cccacagctt tctggtggcg acagccgctc
      121 aagcgcggcc atggctgcgt cgcccgtgat gccacgggac atgtacacgt cctgtctcat
      181 agagacccgc tcgtgtccta agtagtcggc gatctcgcga gccgaggcac cggcaacatc
      241 catctgtgtg gcgaccaacc ggcggaatgc gtgcgggtgc aggccctgcc atggcgtttc
      301 ccgtaccacc gcccgcagct gcttacgggt gttatccggg tcgcgcaacg aatccctggt
      361 cgacggaaac acccacttgc catgcgcggt ctcgtaccgc ctcaacagca ccgtcataac
      421 ccacgttggc gggcggatag ttctcatgcc cgctttgctt ttcgtgtgcg gctggacaac
      481 caggtgactg ggtcccgttt ggtggtgcag cggttatgag agtgctggtt ggctgatcca
      541 ttgtgcacgg aactgggtcg gggtgagccc gcccagtgat ccgtggggtc ggatggtgtt
      601 gtactcgatc ctccagtcct cggccaggac ctgggcttct agaagcgtgt cgaactgttc
      661 ggtggccagg aactcgtcgc ggaaccggcc gttgaacgac tcgctgtagc cgttttgcca
      721 gggtgagccg ggttcgatga acgcggtggc gacatcactg aatcgacacc agtcccgcat
      781 cgcggcggag gtgagttcgg gtccgttgtc catgcgtagg ttctcaggtt gtcgctcagt
      841 ctcggcgacg atcttgtcca gcaggatggt ggtttcatca gcggtgaacg agcgggctgc
      901 cgcggtggcc agcgcttcgc gggtgaactc atccaccacg ttgcaaaacc ggatctgtcg
      961 gccatcgctg gtgacgtcga cctggaagtc cacccgccca cacttcgtct gggcgggtgg
     1021 cggccagccg gtcaccccgc tgcggaccga tccgctgccg cttgcagacc ctggccggcc
     1081 gggtgagccc ttcgtcggcc cagtagcggc ggatccgctt gcggttgacc accaggccgt
     1141 cgctgcgcag cagccagtag gccttgcgcc agccccagcg tggacgagcc caggcgatct
     1201 ggcgcagccg tgcccgcacg ctggtctgca cgtcgcgggg ttggcgcggg cggcggcgct
     1261 gggtgcagcg atgctggccg accaccacac acgcccgacg ttgagagacc ccgaaccgct
     1321 gcagcagcag ctgcccagca cgacgccgac ggtccgggct caccagtttc cccggttcaa
     1381 ctccttgagc atgtcaatat ctagagcctg ctcggcgacc atcttcttca gccgagcgtt
     1441 ctcccgctcc aagtccttaa gccgcttggc atcctcactt ttcatcccgc cgtactggtt
     1501 acgccaccgg taccaggtcg cctcagtgat ctctagatgc ttgagcacct ccggcagccc
     1561 cacaccctgc ccaagcagct tctcaccctc ccgcagcttg cgaacaagct gctccggcgt
     1621 gtgccaccga cgtcgctggg tcatcgtctt cgagtcctcc cacgcccatc attgggcatc
     1681 ggagtctcac aacagatgga ccactatccg gggacctggt cagccgcgct gcgctgggtc
     1741 tggacggcga ccgtgaccgc acacaactgc tcaccgtcct tgactgcccc accagtccta
     1801 ggcagaaaat attgatcagc gccgccaccc tcggggccct caccgatcgc caggccatcg
     1861 gtgtcctggc ctcgtcgttg atcaccaaac cccgctgcct gaccccattc gagtccaaga
     1921 aactccaagc ggcctctccc agcccgctgc taacggcctc gactgcgctc ctatcgaccc
     1981 gacacctcgc cacctctcaa aggtgacctc gcccgcatca tcctcaccta ccttgctgcc
     2041 acccgccccg aactcgtccc cgtcctgggc cacgccgtac gcctgaccca ggacaccacc
     2101 cgcttcgaac ccgccaccct cgcc
//
LOCUS       DATADN010000983         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_260567, whole genome shotgun sequence.
ACCESSION   DATADN010000983 DATADN010000000
VERSION     DATADN010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260567"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VHH53_12705"
     CDS             <1..736
                     /locus_tag="VHH53_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2291023.1"
                     /translation="DNLGQLRFALLAAAATLVGVVVLTVPWWLRLMRALGEERRARIR
                     TEERAEIAAHLHDSVLQTLALIQQQSDSAREVRRLARGQERELRSWLYGPRGYGLPVS
                     NGAGGTAVAEFAATLNAAAGEVEDTYAVGVQVVVVGDCVLEEPLTALLQASREAMVNA
                     AKHAGVAEVSVYAEVEPGQVHVFIRDRGVGFDPAAVADDRHGLADSIRGRMERHGGTV
                     TLRTAPGEGTEVQLAIARRTCAEESS"
     gene            733..1440
                     /locus_tag="VHH53_12710"
     CDS             733..1440
                     /locus_tag="VHH53_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2291024.1"
                     /translation="MTDDALSPAEAESMPVGVFLVDDHALFRTGVKAELDRAGSADPD
                     LPPVVVVGEGGSVEEAITGIAHLRPDVVLLDVHMPDGGGAEVLRRVRPAQPDVVFLAL
                     SVSDAAEDVIAVIRAGARGYVTKTISGRELVDAVRRVHAGDPVFSPRLAGFVLDAFTH
                     RPGAAPVGDPELDLLTPRERDVLRLLARGYAYKEIAAELFISIKTVETHVSSVLRKTQ
                     LSNRYELSRWASDRRLV"
     gene            complement(1461..>1959)
                     /locus_tag="VHH53_12715"
     CDS             complement(1461..>1959)
                     /locus_tag="VHH53_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291025.1"
                     /translation="LLAAAGCLALLVAVLTVTWVSDGRNQPAVATSAVPPAPGANGAP
                     SAPASPGPAQSQTAQLEQAVRTYYGLLPEDTDAAWQYLGAAERAKAARGFAGYDQFWS
                     QIDQISIRGLTVTGNTVLVNLQFDPENRRPTFERYRLTMGTAPDGRLLIESASRLGTF
                     TLAGG"
BASE COUNT          311 a          624 c          691 g          333 t
ORIGIN      
        1 ggacaacctg ggtcagctcc ggttcgcgct cctcgccgcg gccgcgaccc tggtcggtgt
       61 ggttgtgctc accgtgccgt ggtggttacg gctcatgcgc gcgttaggcg aggaacgccg
      121 ggcccggatc cgcactgagg aacgggccga gatcgctgcg cacctgcacg actcagtgct
      181 gcagaccctg gccctgatcc agcagcagtc cgactcagcc cgggaggttc gccggctggc
      241 ccggggtcaa gagcgagagc ttcgttcctg gctctacggt ccgcgcggct acggacttcc
      301 agtctccaac ggcgccggtg gcactgcggt cgccgagttc gcggcgacgc tcaacgccgc
      361 tgcgggcgag gtggaggaca cgtacgcggt gggtgtccag gtcgtcgtcg tgggcgattg
      421 tgtcctcgag gagcccctga ccgcgctgct gcaagccagc cgggaagcga tggtcaacgc
      481 agccaaacac gccggggtcg ccgaagtcag tgtgtatgcc gaagtcgaac ccgggcaggt
      541 acacgtgttc atccgggacc ggggcgtcgg attcgacccc gccgctgttg ccgacgatcg
      601 gcacggcctg gcggactcga tccgcggcag gatggaacgc cacggtggca cggtgaccct
      661 gcgcaccgca cccggggagg gcaccgaagt gcaattggcc atcgcgcgcc gcacgtgtgc
      721 agaggaatct tcatgaccga cgacgcacta agtcccgcgg aggcggaatc tatgccagtt
      781 ggtgtcttcc tggtcgatga ccacgctctg ttccgcaccg gggtgaaagc tgagctggac
      841 agggctggtt cggcggatcc agacctgcca ccggtggtcg tggtcggaga gggtggatcg
      901 gtggaggaag cgatcaccgg catcgcgcac ctacgtcccg acgtcgtgct cctcgatgtg
      961 catatgcccg atggcggcgg cgccgaggtg ctgcgccgcg tgcgacccgc gcagccggat
     1021 gtggtgttcc tcgcgctgtc ggtatccgat gcggccgagg acgtcatcgc ggtgatccgg
     1081 gccggggcac gcgggtacgt aaccaagacg atctccggtc gggaactggt ggacgcggtg
     1141 cgcagagtgc acgcgggtga cccggtattc tccccgcggc tggccggctt cgtactcgac
     1201 gcgttcaccc accgacccgg ggcggcaccg gtcggtgacc cggagttgga cctgctcacc
     1261 cctcgagagc gcgatgtgct gcggctgttg gcccgtggct acgcctacaa agagatcgcc
     1321 gcagagctgt tcatctcgat caaaacggta gagacccacg tgtcgtcggt actgcgcaag
     1381 acccaactgt ccaaccgtta cgagctgtcc cgatgggctt ctgaccgccg cctggtgtag
     1441 ccacgcaccc tcgaaacgcc tcatccgccc gcgagtgtga aggtaccgag cctggaggcg
     1501 gactcgatca gtaagcgacc gtcgggcgcg gtgcccatgg tgagccggta gcgctcgaag
     1561 gtcggccgcc ggttctctgg gtcgaattgg aggttcacca gcacggtgtt gcccgtcacg
     1621 gtgagaccgc gaatgctgat ctgatcgatc tggctccaga actggtcata gcccgcgaag
     1681 ccgcgagcag ccttggcgcg ctcggcagcg ccgaggtact gccacgccgc gtcagtgtcc
     1741 tcaggcagta ggccgtagta cgtgcgcacg gcctgttcca gctgggcggt ttgggattgt
     1801 gcaggtccag gtgaggccgg tgccgatggt gcaccgttcg cgccgggtgc tggtggcact
     1861 gcagacgtgg ctactgctgg ttggttgcgt ccatcgctta cccaggtgac ggtcagcacc
     1921 gcaacaagca gggcaaggca accggcggcc gccagcaac
//
LOCUS       DATADN010000984         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1482062, whole genome shotgun sequence.
ACCESSION   DATADN010000984 DATADN010000000
VERSION     DATADN010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482062"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            479..>1516
                     /locus_tag="VHH53_12720"
     CDS             479..>1516
                     /locus_tag="VHH53_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX2291026.1"
                     /translation="MMRTGHQPAAQRSGIGRRRFLAGLGVGVAGVLTLDNVEAFAEMV
                     EGPVATSAPDPRFSRMFELPAFADPQSTAVQNAMMDIGKPGGLLDAKDPLSEGPVRLI
                     TNPELSPDNADQDVSSMTAGTTFVGQFLDHDVTFDNASRLGVVTDPAQTPNTRVPRLD
                     LDSVYGGGPSVSPQLYRVSDRATLRFGHGGLFEDLPRNPDMTAIIADARNDENLMIAG
                     LHAAFLLAHNRTVNLRRKNNLPGDPFVHARHTIMHHWQWIVVNEFLPQIVGRGMVQDI
                     LRNGRRFYRPAAGQQSIPVEFQGAVYRFGHSMVRPSYRANLRGDPGGDALTGAPAFFG
                     FIFDRAFDGQSD"
BASE COUNT          276 a          492 c          480 g          268 t
ORIGIN      
        1 tcgggccctg gccggcgctc gcccgcatgg acggcgaggg tgtcgatgcg gggattgtta
       61 tggctggaca tctcacacct tcccgcggtc acgtggtggt tacactgtga ccctacgggg
      121 ctgaccatgc tcgcgcaggt acatttcgtc ggcgttccgg tgaaaggttt cgcttcccgg
      181 actggccgca ggggaaaccc ctatcacgat acgatcaaca agacatcgac atgatgtcag
      241 tttcagtagt tgcaccatcg ctcgccgcgg ccagttgaac ccatttatcg aacgttaacc
      301 tgcacgtccc ttgcggcgct gctgcttgct gcccatagtg cagcaagcgt gcccgagtta
      361 atcgaagcct gaaatgttac gaatcggtta tgtcaggtcg gcagccggcc gacaccacgt
      421 ttcagtgtag gactgccgac ccgcaaataa acaagagctg acagtccgga gggaacgcat
      481 gatgaggacg ggtcaccaac ccgccgccca acgcagtgga atcggcagac gtcgattcct
      541 ggcgggactg ggcgtcggtg tggctggcgt actcacgctg gataacgtcg aggcatttgc
      601 cgagatggtt gagggaccgg tcgctacttc agccccggat cctcggttca gccggatgtt
      661 cgagcttccg gcgtttgccg acccgcagtc cacggccgtc cagaacgcga tgatggacat
      721 cggcaagcct ggcgggctgc tggacgccaa ggaccccttg agcgagggcc cggtccggct
      781 gatcaccaac ccggaactca gcccggacaa cgccgaccag gacgtcagca gcatgaccgc
      841 gggcaccacg ttcgtcgggc agttccttga ccacgacgtt acgttcgaca acgcctcgcg
      901 gctcggcgtg gtgaccgatc ctgcacagac gccgaatacg cgtgttccca gactcgacct
      961 cgactcggtg tatggcggcg ggccgtcggt cagcccgcag ctttaccggg tttcagaccg
     1021 tgccaccctc cggttcggcc acggcgggct gttcgaggac ctgccacgga accccgacat
     1081 gaccgcgatc atcgcggatg cccgcaacga cgagaacctc atgatcgcag gcctgcacgc
     1141 ggcattcttg ctggcacaca accggaccgt gaacctccgg cgcaagaaca acctcccagg
     1201 cgaccccttt gtccacgccc ggcacacaat catgcatcac tggcagtgga tcgtggtgaa
     1261 cgagttcctg ccgcagatcg tcgggcgggg gatggtgcag gacatcctgc gcaacggccg
     1321 gcggttctac cggccggcgg ccgggcagca gagcatcccg gtggagttcc agggcgcggt
     1381 gtaccggttc gggcacagca tggtgcgccc gtcgtaccgg gccaacctgc gcggcgaccc
     1441 tggcggcgat gcgttgaccg gcgccccggc gttcttcggg ttcatcttcg accgggcgtt
     1501 cgacggccag agcgac
//
LOCUS       DATADN010000985         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1482109, whole genome shotgun sequence.
ACCESSION   DATADN010000985 DATADN010000000
VERSION     DATADN010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482109"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..715)
                     /locus_tag="VHH53_12725"
     CDS             complement(281..715)
                     /locus_tag="VHH53_12725"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2291027.1"
                     /translation="MLFSQARKRDVVDTATATRIARIDGFVVLPGPPRVALLRLGKVS
                     GAGTLLAWDDVQGFGPDAVTVATDAVIRPARDAVEQRAEDNDLEILGKRVLTERGMEL
                     GTVTDVDFDPDTGSITTLITKTETIAGERLIGLGSYAAVVSD"
     gene            complement(715..1302)
                     /locus_tag="VHH53_12730"
     CDS             complement(715..1302)
                     /locus_tag="VHH53_12730"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2291028.1"
                     /translation="MTTSTPGLLLRTSELVGRPVVTLGGELVAEIKDIIFESTGGRIA
                     GFTLRNPGLFSRSRKDSLPWSEVHGIGRDAVMVTDETELPAAKQLAPRKQARKANVLK
                     DRVLTDNGRDLGRVVDVVLHCGTRLEVVGYEVEASEALGTAGRRVLIPLPDTVAVSGE
                     NLIVPAAATEFVSEDMAGFGAAVDAFRARLREGTS"
     gene            complement(1299..1436)
                     /locus_tag="VHH53_12735"
     CDS             complement(1299..1436)
                     /locus_tag="VHH53_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291029.1"
                     /translation="MDWWGWLILALVILVVLIAVAAMVQRRRRRGGVIGLGDPGRSDP
                     R"
     gene            1617..>2171
                     /locus_tag="VHH53_12740"
     CDS             1617..>2171
                     /locus_tag="VHH53_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX2291030.1"
                     /translation="MRSTSNPAFRNLPVGGGGYAGFNPSPASYPGTAPGYAGAPPAVD
                     RPMTIDDVVTKTGVTLAVLVATGALTYLSGLWALALPAALIGFVLALVVIFKKVVSPP
                     LILAYAAVEGVFLGGISGVIGTAVGNPGIILQAITGTALVFGGMLVVYKTGAVRVTPR
                     FTKWLIGAMIGVVGLTVVNLIAGFF"
BASE COUNT          376 a          751 c          643 g          401 t
ORIGIN      
        1 caacacgtga gaaaattact gctctcactg catggccagt tccatcctaa gcgttggtga
       61 accggctgca tccattcatc ctgtgcgttg atcacgtcca atccttgaga ccagccgtac
      121 gcatcttccc gaattgatct catgccccgg accaacgcgc gtcttttctg cattcggtcc
      181 caggcctcga caggccgagt ggcaagaagc gctgcccact tacgtcttac gtcaaagtcc
      241 ggcacaacga ctccacgatg ccacgctggc aaaatgggag ctaatccgag acgacggcag
      301 cgtaactgcc aagtccgatc agccgctcac cggcgatggt ctcggtcttc gtgatcaacg
      361 tggtgatcga acccgtgtcc gggtcgaaat ccacgtcggt gaccgttccg agctccatac
      421 cgcgttcggt gagtacccgt ttacccaaga tctccaggtc gttgtcttcg gcgcgctgct
      481 ccacggcatc gcgggctgga cggatcaccg cgtcagtggc gacggtcacc gcgtcgggtc
      541 cgaagccctg cacgtcatcc caggccaaca aggtgccggc accgctgacc ttgcccaatc
      601 gcagcagcgc caccctgggc ggtcccggga gcaccacaaa gccgtcaatc ctggctatcc
      661 gcgtggcggt ggcggtgtcc accacatcgc gcttgcgtgc ttgactgaac agcatcagct
      721 ggttccctcc cgcaaccggg cgcggaaggc gtccacggcc gcgccgaacc cggccatgtc
      781 ttcagagacg aactcggtgg cggccgcggg cacgatgagg ttctccccgg acaccgccac
      841 ggtatccggc agggggatga gcacccgccg tcccgcggtg ccgagcgcct cgctggcctc
      901 cacttcgtag cccacgactt cgagcctggt cccgcagtgc agtaccacgt ccaccacacg
      961 tcccaggtct cgcccgttgt cagtgagtac ccgatccttc agcacgttcg ctttacgggc
     1021 ctgcttacgc ggcgcgagct gcttggcggc cggcagctcg gtctcgtcgg tcaccatcac
     1081 cgcatcccga cctatcccgt gtacctcgct ccagggcagt gaatctttgc gagagcggct
     1141 gaacaggccc gggttacgca gagtgaaacc tgcgatacgt ccaccggtgc tttcgaagat
     1201 gatgtccttg atctcggcaa ccagctcgcc gccgagggtg actactggac ggccgaccag
     1261 ctcactggtc cgcagcaata gcccaggagt tgacgtggtc accgtggatc actccggccc
     1321 ggatcgccga ggccgatcac accgccgcgc cgtcttctgc gctgcaccat ggcagcgacc
     1381 gcgatcagca ccacaagaat gaccagcgcg aggatcaacc agccccacca gtccatccct
     1441 cctcctcacc atactgggcc gggtgctgct gttgcgcaca gtaacgtgat gtgcagtgct
     1501 cccttgcctc ggcgtcacca ggcgggggta aagtccgttt taccggaccg ggaaccggac
     1561 ccgccacagg cgcgttgaac ccggtgacga ccgcaatgcc catcaggagg aacacggtgc
     1621 gctccaccag caaccccgca ttccgcaatc ttcccgtagg gggtggcggt tacgcgggtt
     1681 tcaacccatc cccggcgagc tatccgggca cggcgcctgg ttatgccggt gcgccacccg
     1741 ctgtcgaccg gccgatgacc atcgacgacg tggtcaccaa gaccggcgtc acgctggcgg
     1801 tcctggtcgc caccggcgcg ttgacctacc tcagcggcct gtgggcgctc gccctgccgg
     1861 ccgcgctgat cggcttcgtg ctcgcgttag tggtcatctt caagaaggtg gtcagcccgc
     1921 cgctgatcct ggcttacgcc gcggttgagg gcgtattcct cggcggcatc agcggcgtga
     1981 tcggaaccgc ggtcggaaac ccagggatca tcctgcaggc catcacgggt accgccctgg
     2041 tgttcggcgg aatgcttgtg gtctacaaga ccggggccgt cagggtgacc ccgcggttta
     2101 ccaagtggtt gatcggcgcc atgatcggtg tcgtcggtct gacggtggtc aacctcatcg
     2161 caggcttctt c
//
LOCUS       DATADN010000986         1544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1182887, whole genome shotgun sequence.
ACCESSION   DATADN010000986 DATADN010000000
VERSION     DATADN010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182887"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1362)
                     /locus_tag="VHH53_12745"
     CDS             complement(157..1362)
                     /locus_tag="VHH53_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291031.1"
                     /translation="MTIAVLDMDIRNPEGGEWSDRVERLDCYALGSANLARYAGLIIT
                     PSVDQEYLTRTRHVIRAYLDGGGVVVFGGHLNGDWLPGASAFIPLANRSLAGYRVAEV
                     ADHPIFRGKQPDELTFRRGVAGFFARGHHPIPAGAEVLTRLSGGEPATYLDRVSTAGT
                     IVVQATSDLLGYCGEALLDWIATESAQRRAATAGRTRGFAAVYGGSAQHHRALTEPKY
                     AQHLSGGLIYLPELADTDLTAYRGLVIPERMHRGLLTRAADRITELLEAGGTVITFSG
                     GEPIPEFLPGVRWQHRPTNYWWWLERGADLGVRLSAPEHPLFQRLTVRDCTWHYHGVL
                     QPPDGAQVLVELTTGEALLYVDQVSTAGTIVVSTLDPLSHYGGYFMPATERFLDGFLP
                     FVEACPHVS"
     gene            complement(1359..>1544)
                     /locus_tag="VHH53_12750"
     CDS             complement(1359..>1544)
                     /locus_tag="VHH53_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2291032.1"
                     /translation="VLHITHDLALAQRSCDRLVVLQAGQVVEQGSTGVLLSRPEHPYT
                     AALVEAARALSAPRRSV"
BASE COUNT          271 a          501 c          528 g          244 t
ORIGIN      
        1 taatgcgcga accggagata gccctgccag agctggaccg tatggacgat cccacgggtg
       61 gggctgttgc ctgcgcactg ctcatcgtgg gtgcagcgtt gatcacctgc attgttctgg
      121 tggtcatgtg gaccgtcggc gctgcttggt aaaagctcag gaaacgtgtg ggcatgcctc
      181 aacgaacggt aggaacccgt cgaggaaccg ctccgtggcc ggcatgaagt agccgccgta
      241 atggctgagc gggtccagtg tggacaccac gatcgtgccg gcagtggaga cttggtcgac
      301 gtagagcagt gcttccccgg tagtcagctc caccagtacc tgcgcgccgt ccggcggctg
      361 aagcaccccg tgatagtgcc aggtgcagtc ccgaacggtc aggcgctgga acagcgggtg
      421 ctcaggggcc gaaagacgga caccgaggtc agcgccacgt tccagccacc accagtagtt
      481 ggtcggccgg tgctgccagc gcacgcccgg gaggaactcc gggatcggct cgccaccgct
      541 gaatgtgatc accgtcccgc cggcttcgag cagctcagtg atccggtcgg cggcgcgggt
      601 gagcagcccg cgatgcatgc gttcgggaat gactagcccg cggtatgcag tcaggtcggt
      661 gtcggcgagc tcagggaggt agatcagccc accgctgagg tgctgggcgt acttgggctc
      721 ggtgagtgcg cggtggtgct gggcggagcc accgtaaacg gcggcgaaac cccgcgttcg
      781 gccagcggtc gccgctcggc gctgggccga ctcggtggcg atccagtcga gcagcgcctc
      841 accgcagtag cccagcaagt cactggtcgc ctggacgacg atggtgcccg cggtcgacac
      901 tcgatccaga tacgtcgccg gctcgccacc ggacagtcga gtgagaacct cagcgccggc
      961 cgggatcgga tggtgcccgc gggcgaagaa tccggcgacg ccgcgccgga aggtcaactc
     1021 gtcgggctgc ttgccgcgga agattgggtg gtcggcgacc tccgcgacgc ggtacccggc
     1081 cagcgaccgg ttcgccagcg gaatgaacgc cgacgcgccg ggcagccagt ccccgttcag
     1141 gtggccaccg aagaccacca ccccgccacc gtcgagatag gccctgatca catggcgcgt
     1201 tctggtgaga tactcctgat ccacacttgg cgtgatgatc agtccggcat acctggccaa
     1261 attggccgac ccaagcgcgt agcagtcgag ccgttctacc cggtcagacc actcgccgcc
     1321 ctcagggttg cggatgtcca tgtcgagcac cgcaatcgtc atacgctcct cctcggggca
     1381 gacagcgccc gcgcggcctc gaccagcgcg gcggtgtagg ggtgctcggg ccgagacagc
     1441 agcacgccgg tcgatccttg ctccaccacc tgaccagctt gcagtacaac caaccggtca
     1501 cacgagcgct gcgcgagcgc caggtcgtgg gtgatgtgca gcac
//
LOCUS       DATADN010000987         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_862391, whole genome shotgun sequence.
ACCESSION   DATADN010000987 DATADN010000000
VERSION     DATADN010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862391"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VHH53_12755"
     CDS             <1..1086
                     /locus_tag="VHH53_12755"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2291033.1"
                     /translation="ARPVNGYRPAHQRGPGEGVGEMVGWLDRLQRRYRRIGFVIAVVY
                     KYVDDQGGYLAALITYYAFVSLFPLLLLMTTALGVLLAGHPDLQQQVLQSTLSQFPVI
                     GSQLQRPEQLSGGVTAVVVGIAGALYGGLGVGQAVQNAMDSVWAVPRNNRPDPIRSRL
                     RSLLLLFVLGSAAIATTVLSAVGHAAADLGWFGKVGVTVVTVGINALICLVAFRVTTV
                     RHLSYRDVLPGALTAAVIWQLLQWFGASYVAHTVKSTSATNSVFALVLGLLAFLFLVS
                     VTLVMCAEINVVRVDQLHPRALLTPFTDDVDLTPADRRTYTRRAKAERAKGFQRVSVR
                     FDGSRETTSASSDPEFGDRQNITGEPR"
     gene            1358..>1587
                     /locus_tag="VHH53_12760"
     CDS             1358..>1587
                     /locus_tag="VHH53_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291034.1"
                     /translation="MLVIDRTGVVCGHSDSARSVLPGVATGSEMNEIAPPWLSHAHEQ
                     LVRSASTDVEAAPVVAGAWNGRELEARPTTLPG"
BASE COUNT          237 a          542 c          529 g          279 t
ORIGIN      
        1 gccaggccgg taaacgggta tcgaccagcg catcagcgag gcccgggtga gggggtcggt
       61 gagatggtgg gctggctgga caggctgcag cgacgttacc gtcgcatcgg ctttgtcatc
      121 gcggtcgtct acaagtacgt cgatgaccag ggcggctatc tggccgcgct gatcacctac
      181 tacgcgttcg tatcgctgtt tccactgctg ctgttgatga cgaccgcctt gggtgtgtta
      241 ctcgccggac acccggacct ccagcagcag gtgctgcagt ccacgctcag ccagttcccg
      301 gtcatcggca gtcagttgca acggccggag caactcagcg gcggcgtcac ggccgtcgtc
      361 gtcggcatcg cgggcgcgct ctacggcggg ctcggcgtgg ggcaggccgt gcagaacgcg
      421 atggactcgg tgtgggcggt gccgcgcaac aaccggcccg acccgatccg gtcgcgcttg
      481 cgcagcctgc tgctgctgtt cgtgctgggc tcggcggcca tcgccactac ggtgctctcg
      541 gcggtcggcc acgcggccgc cgacctcggg tggttcggca aggtcggcgt caccgtcgtc
      601 acggtgggaa tcaacgcgct catctgcctg gtggcgtttc gcgtcaccac agtgcgtcac
      661 ctctcctacc gcgacgtgct gcccggcgcg ctgaccgcag cggtgatctg gcaactgctg
      721 cagtggttcg gcgccagcta tgtcgcccac accgtcaagt cgacgagcgc caccaacagc
      781 gtgttcgccc tggtgctcgg cctactggca ttcctgttcc tggtgtcggt gacgcttgtg
      841 atgtgcgcgg agatcaacgt cgtgcgcgtc gaccagctgc atccccgcgc gctgctgaca
      901 ccgttcaccg acgacgtcga cctcaccccc gccgatcgcc ggacgtacac gcgcagagcg
      961 aaagccgaac gggccaaagg ttttcagcgc gtcagcgtgc gtttcgacgg ttctcgcgag
     1021 acgacttctg cttcgtcgga cccagaattc ggtgatcgtc agaacatcac cggcgagcca
     1081 cggtagagtc ggcgtgtcag gggttgagcc cacgcctcgc agcaagtgtc cattgtctgc
     1141 aggatcgcca tgcttaccaa ttccctttcc ggggcaggcg ccccctcacc ccgattcacc
     1201 cacaggcggt tcgcctgtcc ccgcggtacc gtcggaccta cgggggtcca ccatcggcaa
     1261 tgcagcggga ggctgaccat tgggcagtga cggatccgtg aaaagcgcgg cgccacggcc
     1321 tcccggcgtg accgcctggc actccgtacc gcacccggtg ctcgtgatcg accggaccgg
     1381 cgtcgtctgt ggccacagcg actccgcccg gtcggtcctg cccggtgtgg cgaccggcag
     1441 cgagatgaac gagatcgcgc caccatggct gtcgcatgcg cacgagcagc tggtccggtc
     1501 cgcgtcaacc gacgtcgagg cggcgcccgt cgtggccggc gcgtggaacg gccgcgaact
     1561 cgaggcgagg cccacgacgc ttccggg
//
LOCUS       DATADN010000988         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_722697, whole genome shotgun sequence.
ACCESSION   DATADN010000988 DATADN010000000
VERSION     DATADN010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722697"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1072
                     /locus_tag="VHH53_12765"
     CDS             104..1072
                     /locus_tag="VHH53_12765"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX2291035.1"
                     /translation="MHEFLTQQAISTPPRHNPVLGIMGRGSDGPVAVAAFIASQREDH
                     GIPYATACRALGVSQSWFYKWQAGTVPPRAARRKKLKVEISRLFRGHDGTYGAPRITA
                     DLRDAGWRVSENTVAALMREQGLAARRRKRRRSTTRSGKGRWRAPDLVERDFAAEQIN
                     EKWYGDGTEIPTGQGKLHLASVLDMGSRRVVGFALSEHHNAELAYGALAMAVAVRGGQ
                     VPGVIMHTDCGSEYTAGLFRAACQRLRIRQSMGRPGSALDNAVIEAWHSTLTFELRSL
                     ETFATKAAARARVPAWIDDYNRHRRHSACGMLSPVDYELAQAQQVA"
     gene            complement(1553..1987)
                     /locus_tag="VHH53_12770"
     CDS             complement(1553..1987)
                     /locus_tag="VHH53_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2291036.1"
                     /translation="MAQSVPDDQNDNRAAEEIVLGVDTHKDLHVAAMITSLGALREDK
                     TFPATAAGYQALLAWAGTFGVLRRAGVECTGSYGAALARHLRAGGIEVIEVNQPDKAT
                     RRRQGKTDSLDAQAAARAVLSGRATGSAKAGDGPVEMLRMSS"
BASE COUNT          394 a          779 c          805 g          377 t
ORIGIN      
        1 acccgcaccc gcgtcgctct gttgcccatg ttccgctcct ctccagatgt gcttgatgga
       61 gagaagtctc gtcagcagac gagccggatt atgccgagcg gaggtgcatg aattccttac
      121 ccagcaggcg atctcgacgc cccctcggca taatccggtg ctcggcatta tgggtcgagg
      181 aagcgatggg ccggtagccg tggccgcctt catcgcttcc cagagggaag atcacggaat
      241 tccgtatgcc accgcgtgcc gggcgctggg agtgtcccag tcctggtttt acaagtggca
      301 ggccggcacg gtgccgccgc gggcagcgcg ccggaaaaag ctgaaagtcg agatcagtcg
      361 gctatttcgc ggtcacgacg gcacctacgg cgcaccacgg atcaccgcgg atctgcgcga
      421 tgcgggctgg agggtcagcg agaacaccgt cgcagcgctg atgcgcgagc agggccttgc
      481 agcaaggcgc aggaaacggc gcaggtccac cacccggtcc ggcaagggac gctggcgtgc
      541 cccggacctg gtcgaacggg acttcgccgc cgagcagatc aacgagaaat ggtacggcga
      601 cggaaccgaa atccccaccg ggcagggcaa gctgcacctg gcttcggtgt tggacatggg
      661 ctcacggcgg gtggtggggt tcgccctgtc cgaacaccac aacgcggagc tggcctacgg
      721 ggcgctagcg atggcggtag cggtgcgcgg cggccaggta cccggcgtga tcatgcacac
      781 cgactgcggc agcgagtaca ccgcgggcct gttccgcgct gcctgtcagc gtctgcggat
      841 ccgccagtcg atggggcggc cggggtcagc cctggacaac gcggtgatcg aggcatggca
      901 ctccacgctg accttcgaat tgcgctcgtt ggaaaccttt gccaccaagg cggccgcccg
      961 ggcccgggtc cccgcctgga tcgacgacta caaccgacac cggcggcact ccgcgtgcgg
     1021 catgctcagc ccggtcgact atgagctcgc ccaggcacag caggtcgcat gaccaccgac
     1081 cggccccgct ctgcgggcag aacaacatgg aggcggctgc gccgccgctg gtcttgtgat
     1141 gcccgcgaag gatacggggc cgcccctcag gcctccaggg tgctccgcat cgcgctacgc
     1201 gcgacgcacc ttcggcgcgc cctggacccc cgagcctccg cggcccctcg ggcagcaacg
     1261 cgggcaggcc ccgcctgccc caccgcaggc gcgggcacca caagaccagc caaccgttca
     1321 acatcactgc aaccgaagtc tccacggttc gaggggattg acggtcgcag ggtgtaggcg
     1381 gcggcgctgg tggtgtcttg gggtgtgtcg ttgtccaggt gcgcgcagtg acggatcagc
     1441 cgcaaggtgc tcagtccaga cagtgtttcg cgcagctggg ggtcggccgc gacgagcaca
     1501 gctttgagtt ggttgatggt ctgggcgcgg gccttgatgg cggaggactt ggctagcttg
     1561 acatccgcag catctccact ggcccgtcac cggctttggc gctaccagtg gcccggccgc
     1621 tgaggactgc tcgggcggcg gcttgggcat cgagactgtc ggtcttaccc tgccggcggc
     1681 gggtggcctt gtcgggctgg ttgacttcga tcacctcgat gccgcctgcc cgcaggtgac
     1741 gggccagcgc ggcaccgtag gagccggtgc actccacgcc ggctcgccgc agcaccccga
     1801 acgtgcccgc ccaggccagt aacgcctgat agccggcagc ggtggccggg aacgtcttat
     1861 cctcccgcag cgcccccaac gaggtgatca tcgctgccac gtgtagatcc ttgtgagtat
     1921 ccacccccag cacgatctcc tcggccgcgc ggttgtcgtt ctggtcgtcc ggaactgatt
     1981 gggccatgct gggcatggtc gtctgtctcc tgatcgccac ggaatggatg gccatcgccg
     2041 gaccggcgcg ggcggtcaga actgtgacgg tgccctgttg atggcaaggc ccctatcggg
     2101 acacgcccac ccggtccggc ggcgcaagca ccaccccaag ccatggccga cagatcaaag
     2161 ccaaggcatg tcagccagtt gcgttgcggg tcagaccacg acctgggatg gcacgccggc
     2221 gatcctcaca accagatccg gacctgggac ggaaccacgc cgatcctcac agttgcatcg
     2281 gcggaatgta ggtatggatg gccggtgtgg agccgtgatc agatggtgtg ggcgaggtcg
     2341 gcgaagaccg ccggg
//
LOCUS       DATADN010000989         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_429956, whole genome shotgun sequence.
ACCESSION   DATADN010000989 DATADN010000000
VERSION     DATADN010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429956"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VHH53_12775"
     CDS             complement(<1..638)
                     /locus_tag="VHH53_12775"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX2291037.1"
                     /translation="MAVLAVAAAAIWAVLGSSLFDVRSVQVAGARELPVEDVRAAAAV
                     ALGTPMLRLDTEGIEARVAAVPRVATVQVRRALDGTVWVELTERTPAAIVRRGNGVHL
                     VDATGTDYAVVPLAPPGLPELRVARVGPRDAATVAALTVLTGLPQPLRAQVLVVAARS
                     PADVVLRLNTGQGGNGREVRWGGVEQGDRKASVLGPLLTQPGKIYDVSSPTLP"
     gene            complement(893..2389)
                     /gene="murC"
                     /locus_tag="VHH53_12780"
     CDS             complement(893..2389)
                     /gene="murC"
                     /locus_tag="VHH53_12780"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX2291038.1"
                     /translation="MTAGFPPPPPALPPQLTRTHLIGIGGAGMSGVARIVLARGGAVS
                     GSDGKDSRALLALRALGAKVTVGHAAVNLDQLGTDPTAIISTKAVHQTDPNNPELVEG
                     ARRGIPVIHRSAALAALMNGYRVACVTGTAGKTSTTSMLTVALQHCSADPSFLIGGDL
                     TSSGSGAHHGSGDTFVAEADESDATFLAYSPDVAVVTNVEADHLDHHGTVEAYAASFD
                     AFVARLTPDGVLIANADDPGSAALADRAAASGVRVRRYGRLATAPAGARILAYTPDGG
                     RGVATVALTGPDGVAHPVRLEVAVPGEHMASNAVAALLAGLELGAPLPGLLAGLAGFG
                     GVRRRFEFIGRAAGVRVYDDYAHHPTKVAAALRGARQVVGQGRLVVAFQPHLYSRTRD
                     FAAEFGTALALADVVLLLDVYGAREDPLPGVTGALIAQAVPLPAGCVHYEPYWAQVPV
                     LLADLARPGDVVITMGAGDVTVLGRELMAELERRCTNQSDAVIWAGQP"
BASE COUNT          370 a          879 c          833 g          313 t
ORIGIN      
        1 ggcagggtcg ggctggagac gtcgtagatc ttgccgggtt gggtcagcag cgggccgagc
       61 accgacgcct tgcggtcacc ttgctccaca ccgccccagc gaacctcccg tccgttacct
      121 ccctgccccg tgttcagccg cagcacgaca tcggccggag agcgcgcagc caccacgagc
      181 acctgggccc gcagtggctg gggcaaccca gtcagcaccg tgagcgcggc gaccgtggca
      241 gcatcccgcg ggccgacgcg ggccacccgt agctcgggca ggccaggtgg cgccagcggc
      301 accaccgcat agtcggtacc ggtggcgtcg accaggtgca caccgttgcc gcgacgaacg
      361 atcgcggccg gagtccgctc ggtgagctcg acccacaccg tgccgtccag ggcccgccga
      421 acctgcacgg tcgcgacccg cggcaccgcg gccaccctgg cctcgattcc ctcggtgtct
      481 aaccgcagca tcggagtgcc cagcgcaacg gccgccgcag cccgcacatc ctcaacagga
      541 agctctctgg cgcccgccac ctgcaccgaa cggacgtcga acagcgagct acccagcact
      601 gcccagatcg cggccgcggc cacggccagc acagccatcc ccgcgagcaa ccaaggccac
      661 cagcgtcggg cacgcatcgg cgaccgctgc gggaccggac gctgctcgtt atcaggcccc
      721 gcggcgggtt ggggcgcttg ccgagcgcgc gatgccgacc gccctgcgcc accgccgccg
      781 gtagtcctgg atcggggagc tgacggacga cgttcccgca aagtcgctgc aggtcgtggc
      841 gtatcgcgcc ggggccagcg tcgggcgccc gaagcgccgg gagcaccggc agtcacggct
      901 gccctgccca gatgacagcg tctgactgat tggtgcagcg gcgctccagc tcggccatca
      961 actcgcggcc gagcaccgtc acgtcaccgg ctcccatggt gatcaccacg tcgccgggtc
     1021 gggccaggtc ggccaacagt acgggcacct gcgcccagta cggctcgtag tgcacgcatc
     1081 cggccggcag cggtaccgct tgcgcgatca gcgctccggt gacaccgggc agcggatcct
     1141 cccgggctcc atagacatcc agcagcagca ccacatcggc cagcgccagc gcggtgccga
     1201 actcggcggc gaaatcgcgg gtgcgggagt acaggtgtgg ttgaaaggcc acgaccaacc
     1261 gtccctgccc cactacctgg cgggcaccgc gcagcgcggc cgccaccttc gtcgggtggt
     1321 gggcgtagtc gtcgtagacc cgcacaccgg ctgccctgcc gatgaattcg aaccggcggc
     1381 ggactccgcc gaacccggcc aaccccgcca gcaggccggg cagcggtgcg cccagctcca
     1441 gaccggccag cagggccgcg acggcgttgc tcgccatgtg ctcaccgggc accgcaacct
     1501 cgagccggac cgggtgcgca accccgtccg gcccggtcag cgccacggtg gcgacacccc
     1561 gcccgccgtc gggggtgtac gccagtatgc gggcaccggc aggtgcggtg gcgagcctgc
     1621 cgtagcggcg tacccgcacg ccactggcgg cggcccggtc cgccagcgct gccgagccgg
     1681 ggtcgtcggc gttggcgatc aacacaccgt ccggcgtcag ccgggccacg aaggcgtcaa
     1741 agctggcggc gtaggcctcg acggtgccgt ggtggtccag gtggtcggcc tcgacattgg
     1801 tgaccacggc gacgtcgggc gagtaggcca ggaaggtggc gtcgctttca tcggcctcag
     1861 ccacgaaggt gtccccactg ccgtggtggg ccccggaccc ggagctggtg agatcgccac
     1921 cgatcaggaa tgacgggtcg gcactacagt gctgcaacgc gaccgtgagc atcgacgtgg
     1981 tggaggtctt gccggcggtg ccggtgacgc aggcgacccg gtaaccgttc atcagtgccg
     2041 ccagcgccgc ggagcggtgg atgaccggga tgccccgccg ggctccctcg acgagttccg
     2101 gattgttcgg gtcggtctgg tgcactgcct tggtgctgat gatcgcagtg ggatcggtgc
     2161 cgagctggtc gaggttgacc gccgcgtgcc cgacggtcac cttggcaccc aacgcccgca
     2221 aggcaagcag cgcccgagag tccttgccgt ccgaaccgga caccgcgcca ccgcgggcca
     2281 gcacgatccg ggccacccca ctcatcccgg cacccccgat gccgatcagg tgagttcggg
     2341 tgagttgcgg tggcaatgct ggtggtggag gtgggaatcc ggcggtcatc cggcg
//
LOCUS       DATADN010000990         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1482615, whole genome shotgun sequence.
ACCESSION   DATADN010000990 DATADN010000000
VERSION     DATADN010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482615"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VHH53_12785"
     CDS             complement(<1..491)
                     /locus_tag="VHH53_12785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX2291039.1"
                     /translation="MTDVEPAEVGLDAARLRRIDEHFARYVDDGRLPGWLVLVSRRGR
                     IAHLSTYGQRDIEAGLPVQPDTLFRIYSMTKPVTSVAAMMLYEQGAFQLTDPIGRFLP
                     EFADMRVFVKGTAAKPVTVPATEPIRVWHLLTHTSGLTYGFHHANPVDAMYRAAGFES
                     AASP"
     gene            complement(488..1549)
                     /gene="hisC"
                     /locus_tag="VHH53_12790"
     CDS             complement(488..1549)
                     /gene="hisC"
                     /locus_tag="VHH53_12790"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005442877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX2291040.1"
                     /translation="MTARTRADLDLLPLYVPGRDIPGAVKLASNEVSEGPLPSVAAAI
                     ADAAASVNRYPDNGVTALRRRLAARLDVPQPHLALGSGSVALCQQLVQATCGHGDEVL
                     FAWPSFEAYPLIARVAGARPRTVPLRPDHAHDLPAMLGAITPATRLVFVCNPNNPTGT
                     AVRSDELVAFLDALPDGVLVALDEAYFEFVTDSAVPDGVVLFRNDLRRDNLAVLRTFS
                     KAYGLAGLRVGYCVAPEPIITALGKVYVPFSVNSLAQVAALASLDVTEEMLARCQRIA
                     AERDRVRAELVAMGYPVPDSQANFVWLPLGEHTDAFDEHCLEHKVVVRGISGAGARVT
                     VGNPAENEAFLAAVRSFRA"
     gene            1710..>1954
                     /locus_tag="VHH53_12795"
     CDS             1710..>1954
                     /locus_tag="VHH53_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2291041.1"
                     /translation="MTQRSLEEIPLSDGGELHLTVAAPESAVRGGIVVVPDARGVTDT
                     VWELAEGLAGEGWLAVIPHLYHRCGVDELPEDGDPEQV"
BASE COUNT          327 a          648 c          682 g          297 t
ORIGIN      
        1 ggtgaagcgg cggactcgaa cccggctgcc cggtacatcg catcaaccgg gttggcgtgg
       61 tgaaacccgt aggtcaagcc cgaggtgtgg gtgagcaggt gccagacccg gatcggctcc
      121 gtcgcgggca ccgtgaccgg cttggcagcg gtgcctttga cgaagacccg catgtcggcg
      181 aactcgggga ggaaccggcc gatcgggtcg gtcaactgaa atgcaccctg ctcgtagagc
      241 atcatcgcgg cgaccgaagt gaccggtttg gtcatcgagt aaatccggaa cagggtgtcc
      301 ggctgcaccg gcaacccggc ctcgatgtcg cgctggccgt aggtcgacag gtgcgcgatc
      361 cgcccgcgcc ggctgaccag caccagccag cccggcagcc gcccgtcgtc gacgtagcgg
      421 gcgaagtgct cgtcgatccg gcgcagccgc gcggcgtcca gcccaacctc ggcgggctcg
      481 acgtccgtca tgcccggaag cttcggacgg cggcgaggaa ggcctcgttc tcggccggat
      541 tgcctaccgt gacgcgggca ccggcgccag aaatcccgcg taccacgact ttgtgctcca
      601 ggcagtgctc gtcgaaggcg tccgtgtgct ctcccagcgg cagccagacg aagttggcct
      661 gcgagtccgg cacggggtac cccatcgcga ccagctcggc tcgcacccgg tcgcgttcgg
      721 cggcgatccg ctggcaacgg gccagcatct cctcggtgac gtcgaggctg gccagggcgg
      781 caacttgggc cagtgagttg acgctgaacg gcacgtacac cttgcccagc gcagtgatga
      841 tcggctccgg cgcaacgcaa taccccaccc gcaggccggc caacccgtag gccttggaga
      901 aggtccgcag caccgcgagg ttgtcccggc gcagatcgtt gcggaacagc accacgccgt
      961 cgggcaccgc ggaatcggtg acgaactcga agtaagcctc atcgagagca accagcacgc
     1021 cgtcgggcag cgcgtcgagg aacgcgacca gctcgtcgct ccgcacggcc gtgccagtgg
     1081 ggttgttggg attgcagacg aagaccaagc gcgtcgccgg agtgatcgca ccgagcattg
     1141 caggcaggtc gtgggcgtgg tcggggcgca gcggcaccgt gcgcggccgg gcaccagcga
     1201 cccgtgcgat caacgggtag gcctcgaagg acggccaggc gaacagcacc tcatcaccgt
     1261 ggccgcaggt ggcttgcacc agctgctggc acagcgccac cgatccgctg ccgagcgcga
     1321 ggtggggctg cggcacgtcc agccgggcgg cgaggcgtcg ccgcaatgcg gtcacgccgt
     1381 tgtccgggta tcggttaacg cttgcggcgg cgtcggcgat cgctgcggcg acactgggca
     1441 acgggccctc ggacacctcg ttactggcca gcttcaccgc gcccgggatg tcgcgaccgg
     1501 ggacatagag cggcaacaag tcgaggtctg ccctggtgcg cgcggtcatt ccggctcctc
     1561 tccgtggccg catggcggca gccgggaacg gaccttcacc ctaaccgcca caaaggttca
     1621 ccagacaggt gatcttctgg ggtgtcgcta agtgggccgg tacgctgccc ggtcacctgg
     1681 tgttcaccgt cgagtagtgg gatgggccat tgacgcaacg tagcctcgaa gagatcccgc
     1741 tctccgacgg cggtgaactg catctgactg tcgctgcacc agaatccgcg gtccgtggtg
     1801 gcatcgtcgt cgtacctgat gcccgcggcg tcaccgatac cgtctgggag ctcgccgagg
     1861 ggctggccgg cgagggctgg ctagcagtga tcccgcacct ctatcaccgt tgcggtgtcg
     1921 atgagcttcc cgaggacggc gatccggaac aggt
//
LOCUS       DATADN010000991         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_429980, whole genome shotgun sequence.
ACCESSION   DATADN010000991 DATADN010000000
VERSION     DATADN010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429980"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..1401)
                     /locus_tag="VHH53_12800"
     CDS             complement(409..1401)
                     /locus_tag="VHH53_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEX2291042.1"
                     /translation="MTQDDLLYRFRLRAFAMAAELGNVRAACRAMGIHPSTYYRWKRQ
                     LDRHGPELLRPRERRVPRMANATSPLIEQRVVAFALGHPGFGPARVAAELARPKWGGI
                     VLSANGVWRVLRRHGLSTRAKRYGLVAGYAAPPAPERPAPTPERHLDVHHPGQLVQLD
                     CFCIGRLSGTKGTVWQYTAIDVASAYTWATLQVTRRNPSATWTSALARQVAADLASRG
                     WRLERVMSDNASEFRSATFQATITKLKARHTFIRAGRPQTNGCVERVQQTILDECWKP
                     AFARYLIPKQTGLRLDLERYLRYYNTERAHTGRWTRGRTPNEVLGKAKLWQKTR"
BASE COUNT          268 a          503 c          594 g          279 t
ORIGIN      
        1 tctaaaatca tgtccagccc tctctataaa cagatgacca tccgcagctg ggataccaca
       61 gcaagactgc tggagttgat gaatgccatg gaacgctaaa gaggcctcgc ctcttcgaat
      121 cgacttcaat tcccaaagct acttgccaat gagatggctg gagtaccaag gccttgcatt
      181 cctgctgggg aaagcaccga ggtcaggagc aaggctcgca catggagaca gcggactccg
      241 agaactgagg cggtcagtca cggtcgctcg agacgaccgt tgcgctgggc tccggctccc
      301 tgacctgcgt gggggaggag gccgaaactg catggcatgc aaggggtcat agctgggtca
      361 gtggcgttcg ccggctagtc tgtcctgtcc ccgagttgtg gcgacgcatc agcgtgtctt
      421 ctgccacagt ttggccttcc caaggacctc gttgggggtc cggccgcggg tccagcggcc
      481 ggtgtgggcg cgttcggtgt tgtagtagcg gaggtagcgt tccaggtcga gccgcagccc
      541 ggtctgcttg gggatcaggt agcgggcgaa ggctggcttc cagcactcat ccaggatggt
      601 ctgctggacc cgctcgacac agccgttggt ctggggcctg ccggcccgga tgaaggtatg
      661 ccgagccttg agcttggtga tggtggcctg gaaggtggcc gagcggaact cgctggcgtt
      721 gtcgctcatc actcgttcca gccgccagcc gcggctggcg aggtcggcgg cgacctggcg
      781 ggccagggcg ctggtccagg tagccgaggg attgcgtcgg gtgacctgga gggtggccca
      841 ggtgtaggcc gaggcgacgt cgatggcggt gtattgccag accgtgccct tggtgccact
      901 gaggcggccg atgcagaagc aatcgagctg gacgagctgg ccaggatgat gcacatccag
      961 atggcgctcg ggcgtcgggg ccggccgttc aggagctggc ggcgccgcgt agccggcgac
     1021 cagcccgtag cgcttggccc gggtcgagag gccgtggcgg cgcagcaccc gccacacccc
     1081 gttggctgag agcacgatcc caccccactt gggtcgagcg agctcggcgg cgacgcgagc
     1141 tggcccgaac ccgggatggc ccagggcgaa ggcgaccacc cgttgctcga tcagggggct
     1201 ggtggcattg gccatgcgcg gcacccgccg ctcccgtggg cgcaggagct cagggccatg
     1261 ccggtccagc tgccgcttcc agcggtagta ggtggaggga tggatgccca tggctcggca
     1321 ggcggcgcgc acgttgccga gctcggccgc catcgcgaag gcccgtagac ggaaccggta
     1381 cagcaggtca tcctgcgtca cggcgacgcg tcctttctcc tcgacttggt cgtcaaggcg
     1441 aaggatgcgt cgccgttcag cacaactggg ggatgtgccc cggtgtcgct acatcccggg
     1501 gataggacag gctagtgcat ggccattagg tcgtccgtcc tcggccgcca gctgcggcca
     1561 gctaggacgg cccgagggcg agctgccggc ctgcggcgct ccaccttgac cccgggccgg
     1621 gccgaatggt ccccaggaca cggg
//
LOCUS       DATADN010000992         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_723154, whole genome shotgun sequence.
ACCESSION   DATADN010000992 DATADN010000000
VERSION     DATADN010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723154"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..610)
                     /gene="nirD"
                     /locus_tag="VHH53_12805"
     CDS             complement(269..610)
                     /gene="nirD"
                     /locus_tag="VHH53_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEX2291043.1"
                     /translation="MTAQVRAHWTAVCRYDDIIPERGVAALVDGAVVAVFRTHDGTLH
                     ALSNLDPFSSASVLSRGIVGDRGGRATVASPIYKQVFELATGLCLDDPKVSVPTYPVR
                     VVDGIVEIGAP"
     gene            complement(645..>1598)
                     /locus_tag="VHH53_12810"
     CDS             complement(645..>1598)
                     /locus_tag="VHH53_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase (NAD(P)H)"
                     /protein_id="HEX2291044.1"
                     /translation="PRSHILDGEQATLQDTNDHFLANLQRNGTYSVIPRIPGGEVTPE
                     KLIVIGEVARDFGLYTKITGGQRIDMFGARVEQLPAIWRRLVDAGFESGHAYGKALRT
                     VKSCVGTDWCRYGVQDSVGMAISLELRYRGLRAPHKLKSAVSGCARECAEARSKDFGV
                     IATETGWNLYVCGNGGFRPRHADLLVSDVDTETLVRTIDRFLMFYVRTADRLQRTAPW
                     LEDMEGGLGHLRDVIVHDSLGICAELDAAMEAHVAGYSDEWRGVLEDPDKLARFTSFV
                     NAPGTPDPTIEFVQERGQPTPAHRRPGQPVMIAGPKLAVVR"
BASE COUNT          254 a          562 c          525 g          257 t
ORIGIN      
        1 ggcgtgcatc acctcagcac ccttgcgcac cagtgaagcc cccagttctg cggctctccg
       61 ggcagcagtg acaccgacgg tgaaccccgc caacggcagg ggttcgggtc gcggcctcgg
      121 cgatggtgcg gtcatgtcgg caccatcgtg cgtcctgccg atgccggttc agcggcgagt
      181 gcttcagcgg gccgtgcaag agcgccggac acaccatgca ccagcgcggg acacaccgtg
      241 catgaggcgc ggacacgcgg agagggggtc atggtgcacc gatctccacg atcccgtcga
      301 ccacccgcac cgggtacgtc ggcaccgaga ctttcgggtc gtccaggcat agcccggtgg
      361 ccagctcgaa cacctgttta tagatcggcg acgccaccgt cgcccggccc ccacgatctc
      421 cgacgatccc tcttgagagc accgatgcgc tgctgaacgg gtccaggttc gatagtgcgt
      481 gcagcgtgcc gtcgtgggtg cggaataccg ccaccacggc cccgtcgacc agggccgcca
      541 ccccccgttc cgggatgatg tcgtcataac ggcacaccgc ggtccagtgc gcccgcacct
      601 gcgcggtcac gtcgccgcct ccgcgggacc aatcacatcg gcggtcatcg caccactgcc
      661 agtttcggac cggcgatcat gaccggttgg ccgggccggc gatgggccgg cgttggctgg
      721 ccgcgctcct ggacgaactc gatggtggga tcgggtgtgc ctggcgcgtt gacgaacgag
      781 gtgaagcggg ccagcttgtc gggatcttcc agcacgccgc gccactcgtc ggagtagccg
      841 gccacatgcg cctccatcgc cgcgtcgagc tcggcgcaga tcccgagcga gtcgtgcacg
      901 atcacatccc gcagatggcc cagcccgcct tccatgtcct ccagccacgg cgcggtgcgc
      961 tgcagccggt cggcagtgcg cacgtagaac atcaggaacc ggtcgatggt gcgcaccagc
     1021 gtttcggtgt ccacatcgga taccaggaga tcggcgtgcc gcggccggaa gccgccgttg
     1081 ccgcacacat agaggttcca ccccgtctcg gtggcgatca ccccgaagtc cttgctgcgg
     1141 gcctcggcgc attcccgggc gcaaccggag accgcggact tgagcttgtg tggtgcgcgc
     1201 aaccctcggt aacgcaactc cagggagatg gccatgccca ccgagtcctg caccccgtag
     1261 cggcaccagt cggtgcccac gcaggatttc acggtgcgca acgccttgcc gtaggcgtgc
     1321 ccggactcga acccggcgtc caccagccgg cgccagatcg ccggcagctg ctccacccgg
     1381 gcgccgaaca tatcgatccg ctgcccaccg gtgatcttgg tgtagaggcc gaagtcgcga
     1441 gccacctcgc cgatcacgat cagtttctcc ggggtgacct cccccccggg gattcggggg
     1501 atcaccgagt aagtgccgtt gcgttgcagg ttggccagaa agtggtcgtt ggtgtcctgc
     1561 agcgtggctt gctcgccgtc caggatgtgg ctccgggg
//
LOCUS       DATADN010000993         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1483164, whole genome shotgun sequence.
ACCESSION   DATADN010000993 DATADN010000000
VERSION     DATADN010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483164"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(550..1098)
                     /locus_tag="VHH53_12815"
     CDS             complement(550..1098)
                     /locus_tag="VHH53_12815"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX2291045.1"
                     /translation="MQEAHPGTVVELWTTDQHRLGLKPILRRVWCRRGDRPHAVVQHR
                     YQWCYLYAFVHPPSGRTFWLLLPTVSIIAFNVALAEFAHAVGAGRGKQIILVCDGAGW
                     HVSPQVEVPVGVHLHLLPPYSPELQPAERLWPLTNEPLANRHFQDLEALQTVQAQRCL
                     TLQAMPEVIRAQTNFHWWPQTA"
     gene            complement(1107..1607)
                     /locus_tag="VHH53_12820"
     CDS             complement(1107..1607)
                     /locus_tag="VHH53_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2291046.1"
                     /translation="MPKRLSIQPQLALDALERRYRTARDPVARSHWQMVWLLAQGRPS
                     EQVAAVTGYTANWVRTIAQRYNRHGSAGLGDRRHRNRGSPGLLSAAQRTALASTLDRP
                     PPDGGLWTGPKIAAWMAATLGRPVHPQRGWEMLQRLGLRPKVPRPRHAKADPAAQAAF
                     KKSFPV"
BASE COUNT          302 a          539 c          589 g          301 t
ORIGIN      
        1 ccatcaacgt ccgcaggtag ctctcaaagt caaggcgtgc ggcgtcatcg gcacgatata
       61 gctgcccatg aatgagggcc atcgactgga cccagttttg actctcccta cggttctcat
      121 gctgatagtg cgggcagcgc atgcgagccc ctccgccctc ggctgagacg aggtacatgg
      181 tcgggcagcg tgcgcgcagg aacctcggcg cgctccatcg aagtgggggc atatgaaatg
      241 gtgtgtcaat actccaggtc ctgcccggcg tcaagcgaaa agcctgcgac gttgacaggc
      301 actgggacgg gcgtgcgagg cggtggtgcc gccgctgacc caccgccccg tagcgtcgtg
      361 gtgcggacac ccccgtgagc aggaccctgt ggcgggatga accgcataga cgcgagggca
      421 cgcatgatct ccgcgcgggc tcgcatagga ggggtggtgt tgagggggac tggctctagc
      481 cgaatgccca taatgggatc gtcgccacca ccgttgtggt gatatcaaat ccggctaatc
      541 gctacgggat taggccgttt gtggccacca atgaaagtta gtctgggcac gaatcacctc
      601 cggcatcgcc tggagcgtga gacagcgttg ggcctggacg gtttgcagag cttcgagatc
      661 ctggaagtgg cgattggcca gcggttcatt ggtcaagggc cacagccgtt ccgccggctg
      721 cagctccggt gaatacggcg ggagcaggtg caggtgcacc cctactggta cctccacctg
      781 cggactgaca tgccagcccg cgccgtcgca cacgaggatg atctgtttgc cccgcccagc
      841 gcccaccgcg tgggcgaatt cggcgagggc cacattgaag gcgatgatag agacagttgg
      901 caggagcagc cagaacgttc gccccgaggg gggatggaca aaggcgtaca gataacacca
      961 ctgataccgg tgctggacca ccgcgtgggg gcggtccccc cggcggcacc acacgcgtcg
     1021 taggatgggt ttgagtccca ggcggtgctg gtcggtcgtc cacagctcga cgacagtgcc
     1081 ggggtgggcc tcctgcaccg cttggactac accgggaagc tttttttaaa ggccgcctgg
     1141 gcggccgggt cagccttggc atggcgcggc ctgggcactt tggggcgcaa gccgagccgc
     1201 tggagcatct cccagccccg ctgagggtgc acaggccggc ccagggtagc cgccatccaa
     1261 gcggcgatct tcggcccggt ccacagccct ccgtcaggcg gcggccggtc gagcgtgctc
     1321 gccagagccg tacgctgtgc cgccgagagc agccctgggc tgccgcggtt ccgatgacgg
     1381 cgatcgccga ggcccgccga accgtggcgg ttgtagcgct gggcaatggt gcgcacccag
     1441 ttggccgtgt agccggtgac ggcagccacc tgctccgagg ggcggccttg ggcgagcagc
     1501 cagaccatct gccagtggct gcgcgcgacc ggatccctgg cggtgcgata gcgtcgctcc
     1561 agtgcgtcaa gcgcgagctg tggttggatt gagagtcgtt tcggcatgta cccactatac
     1621 acccggactt ggtatcagct cttcgttgat gccttcgcgc aggcctttcc cgacagcctg
     1681 aatatcctcc tgctggacaa cagtggggca catacatccc agcggctcac c
//
LOCUS       DATADN010000994         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1483397, whole genome shotgun sequence.
ACCESSION   DATADN010000994 DATADN010000000
VERSION     DATADN010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483397"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VHH53_12825"
     CDS             <1..371
                     /locus_tag="VHH53_12825"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX2291047.1"
                     /translation="KGQVLKQDPAGGAEVKAGTTVTLTVSRGNQLEMPDLQGQTPTQA
                     QNTLQRLGWTGTLRVATAPTNDPDLVGRILRQDVPAGTGFARDQTITVTVGQSSSSST
                     TSPSDGDEGTFDSPFPSNPN"
     gene            complement(409..1053)
                     /locus_tag="VHH53_12830"
     CDS             complement(409..1053)
                     /locus_tag="VHH53_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX2291048.1"
                     /translation="MRILVIDNYDSFVYNLVHYLAQLGAQCVVRRNDAVEAGDVADVD
                     GVLISPGPGTPERAGSSMDIVRECAEQGLPLLGVCLGHQAIGAVWGATVARAPELLHG
                     KTSVVHHDGVAVLAGLPQPFVATRYHSLAVLPETLPEELEVTGHTDSGIVMALRHREL
                     PVYGVQFHPESVLTDGGHRLLGNWLRIAGAEVPEVRVAELETGMRDLASAALVR"
     gene            complement(1259..>1638)
                     /locus_tag="VHH53_12835"
     CDS             complement(1259..>1638)
                     /locus_tag="VHH53_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX2291049.1"
                     /translation="LLSSCDAIVVETVDRGCVYRVLPLRGESADWASKAAQSQCRGVE
                     PLTGSYADVVGKTIVLPSQDEVIAPVPGQPDQPAKHNIELITLTTCHPQFSNRQRLIV
                     HGILVAQYPKVAGQRPAELTAAA"
BASE COUNT          292 a          561 c          537 g          248 t
ORIGIN      
        1 cgaagggcca ggtcctgaag caggacccgg cagggggcgc cgaagtcaag gcaggcacca
       61 cggtgaccct gaccgtgtcg cgaggcaacc agctggagat gcccgacctg caggggcaga
      121 ctccgaccca ggcgcagaac accctgcaaa ggctcggttg gactggcacc ctcagggtgg
      181 ccactgcccc gacgaacgac cctgatctgg taggccggat cctcaggcag gacgtgccgg
      241 cgggtaccgg cttcgctcgc gaccagacca tcacggtcac cgtcgggcag agcagttcaa
      301 gcagcaccac aagccccagc gacggcgacg aggggacctt cgactcgcca ttcccgtcaa
      361 accccaactg actcgcgtgt cgtgccctgg tgtccggtgt gtcgcgcgtt agcgcaccag
      421 tgcggctgag gccaggtcgc gcatcccggt ctccagttcg gccacccgca cctcgggtac
      481 ttcggccccg gcgatacgca gccagttccc cagtagccgg tgcccgccgt cggtcagcac
      541 tgactccggg tggaactgca ctccgtacac cggcagctcc cggtgccgta gggccatcac
      601 gatccccgag tcggtgtggc cggtgacctc gagttcctcc gggagggtct cgggcagcac
      661 cgccagcgag tggtaccggg tcgccacaaa cggctgcggc aacccggcca gcacggcgac
      721 gccatcgtgg tgcaccacag aggtcttgcc gtgcagcagc tcaggggctc gtgcgacggt
      781 ggctccccag accgcgccaa ttgcctggtg ccccaggcac acgcccagta acgggaggcc
      841 ctgctcagcg cactctcgga cgatgtccat gctggagccg gcgcgctcgg gcgtgcccgg
      901 cccagggctg atcagaaccc cgtcgacgtc ggcaacgtcg ccggcctcca ccgcgtcgtt
      961 gcgccggact acgcactgag cacccagctg cgccagataa tgcacgaggt tgtagacgaa
     1021 gctgtcatag ttgtcgatca cgaggatgcg cacgcgggcc agcataggcg tcggccatgc
     1081 cggtggggtg cgctcagccc gtcgtcacgt cattgaatgg caggtggggt tccagccatg
     1141 ggaacacgac gagaaacagc aacgtgacta tcgccgtcgc caacatcagc gcctgtatta
     1201 cgcgtatcac gagtggtcca ggcaggtggc gccagatcca gccgtacatg agccctcctc
     1261 acgcagcagc ggtcagctcg gcaggccgtt gaccggcgac cttcgggtac tgagcgacca
     1321 ggatgccgtg gacgatgagc cgttgccggt tggagaactg cgggtgacac gtggtcagcg
     1381 tgatcagctc gatgttgtgt ttggcgggct gatcgggctg gccgggcacc ggagcgatca
     1441 cctcgtcctg cgacggcaat acgatggtct tacccaccac gtcggcgtag ctgccggtca
     1501 gcggttcgac gccgcggcac tgcgactgcg cggccttgct ggcccagtca gccgactctc
     1561 cacgtagcgg cagcacccgg taaacgcacc cgcgatcgac cgtctccacc acaatggcgt
     1621 cgcaggacga cagtagac
//
LOCUS       DATADN010000995         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_863338, whole genome shotgun sequence.
ACCESSION   DATADN010000995 DATADN010000000
VERSION     DATADN010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863338"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2801
                     /locus_tag="VHH53_12840"
     CDS             <1..2801
                     /locus_tag="VHH53_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX2291050.1"
                     /translation="QAQVRGVAGTWKALTDNVNGMAENLTGQVRSIAAVASAVAGGDL
                     SKKITVETRGEVAALAETINRMVDTLSAFADEVTRVAREVGTEGTLGGQARVPNVAGT
                     WKDLTDNVNFMADNLTSQVRNIAQVTTAVASGDQSKKIDVSARGEILELKTTINTMVD
                     QLSAFASEVTRVAREVGTEGKLGGQAEVIGVSGTWQQLTENVNGLASNLTTQVRAIAA
                     VATAVTAGDLTRQITVETSGEVAELKDNINQMIATLKETIRANQEQDWLKTNLARLSG
                     LMQGHRDLATVATLVMSELAPLVSAQQGAFFLVCRDDDGPPMVEFTAGYGIPDRAAIR
                     SFALGESLVGQAALDKQVILVTDAPPDYATISSGLGWAAPTNLLILPLLFEDQALGVI
                     ELASVNQFTTVHRDLLELLRETIGVNVNTILANSRTEALLAESQRLAQELGSGSRRLQ
                     AQQHELQKSNTELAEKAALLATRNRDIEIKNAEIELARQELEERAGQLAAASRYKSDF
                     MANMSHELRTPLNSALVLAKLLADNLEGNLSPKQVEFAKTIYSAGSDLLQLINDILDL
                     AKVEAGRMYPSLSDVTMTAVVSYVTALCEPLAMEKALHFTVELDPALPPILHTDEQRL
                     QQVLRNLMSNALKFTNEGGVQLRIRLASAAEVTGDALRAASARVAFEVADTGIGIDAD
                     KLSMIFEAFRQADGTTSRRYGGTGLGLSISRQLTELLGGELAVRSEPGKGSVFTLYLP
                     EVYLPGVTEHHRPTAVPGGDALHKGRDKLASSDLVSAARSLPQLHSGPDEAPACFHGE
                     KVLIVDDDLRNVFALTSLLEAYGLQVVFADNGVAAVRALERHDDTALVLMDVMMPELD
                     GNATIAAIRNLSRYADLPIIAVTAKAMKEDRAKSLASGADDYVTKPVDTDLLLGLIST
                     HLATQSEAAVDDEAIVGP"
BASE COUNT          565 a          967 c          938 g          481 t
ORIGIN      
        1 gtcaggccca ggtgcgtggg gtggctggta cctggaaagc gctcaccgac aacgtcaacg
       61 ggatggcgga gaacctgacc ggtcaggtgc gcagcatcgc cgcggtggcc agcgcggtgg
      121 ccggtggtga cctgtcgaag aaaatcaccg tggagacccg cggcgaggtc gccgcactgg
      181 ccgagacgat caatcggatg gtggacaccc tcagcgcgtt cgctgacgag gtgacccggg
      241 tggcgcgcga ggtaggcacc gagggaaccc tgggaggcca ggcccgggta cccaacgtcg
      301 caggcacgtg gaaggacctc accgacaacg tcaacttcat ggccgataat ctgaccagcc
      361 aggtgcggaa catcgcgcag gtgaccaccg cggtggccag cggtgaccag tccaagaaga
      421 tcgatgtgag cgctcgtggc gagatcttgg aactcaagac cacgatcaac accatggtgg
      481 accaactgtc cgcattcgcc tccgaggtga cccgggtggc tcgcgaggtc ggcaccgagg
      541 gcaagctcgg cgggcaggcc gaggtgatcg gcgtatccgg cacgtggcag cagttgaccg
      601 agaacgtcaa cggcttggcg agcaacctca ccactcaggt gcgcgccatc gccgcagtgg
      661 ccacggcggt gaccgccggc gatctgaccc gtcagattac tgtcgagacc tccggcgagg
      721 tggccgagct caaggacaac atcaatcaga tgatcgccac cctcaaggag accatccggg
      781 ccaaccagga acaggattgg ctcaagacca atcttgcccg gttgtccggt ctcatgcagg
      841 gtcaccgcga tctggccacc gtcgcgacgc tggtgatgag cgaactggcc ccgctggttt
      901 cggctcagca aggggcgttc ttcttggtct gccgggacga cgacgggccg ccgatggttg
      961 agttcacggc gggttacggc atccctgatc gggcggcaat acgcagcttt gcgctcggcg
     1021 agtcactggt tgggcaggcc gccctcgaca agcaggtaat cctggtgaca gatgcaccgc
     1081 cggactacgc aacgatcagc tccggcctcg gctgggctgc accaaccaac ctgctcatcc
     1141 tgccgttgct gttcgaggat caggccttgg gggtgatcga actcgcctcg gtcaaccagt
     1201 tcacgacggt gcaccgggac ctgctcgaac tgcttcggga gacgatcggc gtcaacgtca
     1261 acaccatcct cgccaactcc cgtaccgaag cactgctggc cgagtcgcag cggctggcac
     1321 aggagctggg ctcgggttcc cggcgcctgc aggcccagca gcacgagctg cagaagtcca
     1381 acaccgagct ggccgaaaag gctgctctgc tggcgacccg aaaccgcgat atcgagatca
     1441 agaatgccga gatcgagctc gcccgccagg aactcgaaga acgggccggc cagctcgccg
     1501 ccgcatcccg gtacaaatca gacttcatgg cgaacatgtc ccacgagttg cgcaccccgc
     1561 tcaacagcgc gcttgtcctg gccaagttgc tcgccgataa tctggaaggc aatctgtcgc
     1621 ccaagcaggt ggaattcgcc aaaaccattt actcggccgg cagcgacctg ctgcagctga
     1681 tcaacgacat cctcgacctg gccaaggtcg aagccggccg gatgtacccg tcactgtccg
     1741 acgtgacgat gaccgccgtg gtgagctatg tcacagcgct gtgcgagccg ctcgccatgg
     1801 agaaggctct gcacttcacc gtggaactcg atccggcact accgccgatc ctgcacaccg
     1861 acgagcagcg cctgcagcag gtgttgcgca atctgatgtc caacgccttg aagttcacca
     1921 acgaaggggg cgtgcagctg cggatccggc tggcatctgc tgctgaggtg accggcgacg
     1981 cgctgcgcgc cgcgtcggcg cgagtggcct tcgaggtggc ggacaccggc atcgggatcg
     2041 acgccgacaa actctctatg atcttcgaag cgttccggca ggccgacggt acgaccagcc
     2101 ggcgttacgg cggcaccggt ctcggattgt cgatcagccg gcagctgacc gagctgctcg
     2161 gtggcgagct ggcggtacgc agcgaaccag gcaagggcag tgttttcacc ctttatctcc
     2221 ccgaggtcta cctgcctgga gtcaccgaac accaccggcc aacggcggtc cccggcgggg
     2281 atgccctcca caagggacgt gacaagctcg catcatccga cctggtatcg gcagcccgat
     2341 cgctacccca gctgcactca gggcccgacg aggccccggc gtgcttccat ggggagaagg
     2401 tcctgatcgt cgacgacgac ctccgtaacg tattcgcgct gaccagcctt ctggaggcct
     2461 acggtctgca ggtcgtcttc gccgacaacg gcgtggccgc ggtccgcgcg ctagaacgtc
     2521 acgatgacac cgcactggtc ctgatggacg tgatgatgcc ggagctagac ggcaacgcga
     2581 cgatcgcggc gatccgcaac ctgagccgct acgctgattt gcccatcatc gcggtgaccg
     2641 cgaaagcgat gaaggaggat cgagcgaaga gcctcgcctc gggggcggac gactacgtca
     2701 ccaaacccgt cgacaccgac ctgctgcttg gtctgatcag cacacatctt gccacccaat
     2761 ccgaggccgc cgtggatgac gaggccatcg ttgggccgta gctgccggcg cgactcggcg
     2821 gttactcgag gacgtccttc atggcgtcct tggccttgcc cccagcctgc ttgaggtgac
     2881 ccatccctgg tcgatctttc cctctgccac caaccgctcg tcgccggtga ccttgccggc
     2941 ggcctccttg g
//
LOCUS       DATADN010000996         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_430964, whole genome shotgun sequence.
ACCESSION   DATADN010000996 DATADN010000000
VERSION     DATADN010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430964"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VHH53_12845"
     CDS             complement(<1..807)
                     /locus_tag="VHH53_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2291051.1"
                     /translation="MTGLTRLSLSNRAIVALVTVLVVVFGVISTISLRQELLPSIDIP
                     LASVITQYPGASPAVVEQQVTAPIETAVGGITGVTGTSSMSTGGSSVITVDLEYGSDL
                     AELTSQVQRAVQGVTLPADVTPKVVAGSTDSLPVLHLGVSSNLGNDRVAAVLSDDIRP
                     LLTGLDGVAGVTVSGIREPRITVDVDMDKAADRGVSLSSILSALKANGVRVPAGELNP
                     DTEPLTVEVGSQITSVQQLRDLYVTPTVAPGEVPAAAALAAAPPADEPAGV"
     gene            complement(812..1267)
                     /locus_tag="VHH53_12850"
     CDS             complement(812..1267)
                     /locus_tag="VHH53_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX2291052.1"
                     /translation="MTVTGEVGELEHGVEIPVAEETAFPIGSISKAWTGTLAMILVPE
                     RDNKLDAPLDEYLPKLDDLVGRLTLCQLLSHISGFADIPELSTLSPGRYVRARRCLRR
                     TSGAGHATRLGPSGPTCCRETTSSSTRRARTCSGWWRLSSNRPKAEQPG"
     gene            complement(1722..>1918)
                     /locus_tag="VHH53_12855"
     CDS             complement(1722..>1918)
                     /locus_tag="VHH53_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011895475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2291053.1"
                     /translation="RSLRSTNCVKSLALRWCAGMVEATTQFRRVNGHLHLRSLHTALE
                     RHVATENVSVNEHNHISNAA"
BASE COUNT          348 a          609 c          621 g          340 t
ORIGIN      
        1 aacaccggcc ggctcatcag caggcggagc ggcggcgagg gctgcggcgg ccggcacctc
       61 tccgggggcg acagtggggg tcacatagag gtcgcggagc tgctggacag aggtgatctg
      121 actgcccacc tcgacggtca acggctcggt gtcggggttg agctcgcctg cgggcacccg
      181 aaccccgttg gccttgagcg cgctcaagat gctgctcagc gacaccccac ggtccgctgc
      241 cttgtccatg tcgacgtcca cggtgatccg cggctcgcgg atgccggaca ccgtcacgcc
      301 ggccacaccg tcaagcccgg tgagcagcgg gcggatgtcg tcgctcagca cggcggcgac
      361 ccgatcgttg cccaggtttg acgacacccc cagatgcagg accgggaggc tgtcggtgct
      421 accggcgacg accttcgggg tgacgtccgc gggcagcgtc acaccctgca cggcccgctg
      481 aacttgcgag gtcagctcgg cgaggtccga accgtactcc aggtccacgg tgatcaccga
      541 ggatccaccg gtgctcatcg aactcgtacc ggtcacgccg gtgatgccgc caaccgcggt
      601 ctcgatcggc gctgtgacct gctgctcgac taccgccggg gacgcacctg ggtattgcgt
      661 gatcactgag gccaacggga tgtcgatcga aggaaggagc tcctggcgca acgagatcgt
      721 gctaataacg ccgaacacaa cgaccagtac cgtgaccagc gcaacgatcg cgcggttgct
      781 taagctgaga cgggtaagtc cagtcatcga atcaccctgg ctgctccgcc ttgggccgat
      841 tcgagctcag acgccaccag ccggagcagg tccgggcgcg ccgagttgac gaagaagtgg
      901 tctcccggca gcaggtgggg ccggaagggc ccagtcgtgt ggcgtgacca gccccggacg
      961 tcctccgcag gcacctcctc gcccgcacgt agcgccccgg cgacagggtg gacagctcag
     1021 ggatgtcggc gaagccgctg atgtggctga gcagctggca cagggtgagc cggccgacga
     1081 gatcgtccag cttgggcagg tactcgtcca gcggtgcgtc gagtttgttg tcacgttccg
     1141 ggaccaagat catcgctaag gtcccggtcc aggccttact gatcgacccg atggggaagg
     1201 cggtttcctc agcaaccgga atctctactc cgtgttccaa ctcaccgacc tcgcccgtga
     1261 cggtcacacc accacagtga acggcgagct aggcaccgga tacctggtgc gtgtctgcca
     1321 ggcaacgtgg cggccagcca agctgggtcg accggtgcgc ctacaacgcc cccctcgact
     1381 tcacccgttc ggacgttaac attccttggg gctattccca tgggtcacac gttaaggctg
     1441 gacccttcac agagccttag tattagctta aggacatcgg gtagttcggc atccgatcgg
     1501 catcgcagcc gcgccacttg cggcaacaca tccggatcgg cacatatctg cttcatcagc
     1561 cggatgtaga tcttgcatcg acggcgacgc ggtcagtgaa cctcaatatc actgagtgca
     1621 catcgtagcg cgattgatct tgatgacgtc aactgcgaca gcgactcggc ccggaggatg
     1681 tcccgaacct cgtagatttt tggcgacggt ccggtgatca tttaggcggc gttgctgatg
     1741 tgattgtgct cgttgacgct gacattctcg gttgcgacat gccgttcgag cgcggtgtgc
     1801 agcgagcgca ggtgcaggtg accgttgacg cggcggaact gggtggtggc ttcaaccatg
     1861 ccggcgcacc agcgcagcgc caagctcttc acgcagttgg tcgatcgcaa ggatctgg
//
LOCUS       DATADN010000997         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_262192, whole genome shotgun sequence.
ACCESSION   DATADN010000997 DATADN010000000
VERSION     DATADN010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262192"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VHH53_12860"
     CDS             <1..700
                     /locus_tag="VHH53_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2291054.1"
                     /translation="RIRPSTIAAEDLLHDLGAISMIGSDSQAMGRIGEVILRTWQTAH
                     VMKRRMGALPGDPAISDNLRARRYVAKYTICPAVAHGLDGEVGSVEVGKLADLVLWQP
                     AFFGVRPSVVLKGGMIAWAAMGDSNASIPTPQPVLPRPMFGAAATTAAATSVHFVAPA
                     AIQDGLGDRLAVRRRLVAVRDTRALRKADMPLNAALPSIEVDPGTFTVRIDGEVVEPH
                     PVQELPMAQRYFLF"
     gene            700..1386
                     /locus_tag="VHH53_12865"
     CDS             700..1386
                     /locus_tag="VHH53_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX2291055.1"
                     /translation="MVTLGSLLLADSRLPTGGHAHSGGVEAAVERGLLQNESDLALFL
                     AGRLRGSGPVIAAVAAAGCLLTADTGSQWGRWDAAVSARTPSAAVRESSRAQGAALLR
                     TARRVWPSAALEALSEMGRPHRPLVLGAVVAAVGDGPDVAALLALHHLLGGACAAAVR
                     LLGLDPIAVAAVQTRAAEPAEQAALAATWLAREAVVGRDTRLLPSTGTPLPEVLAELH
                     ARTEVTLFAS"
     gene            1376..1996
                     /gene="ureG"
                     /locus_tag="VHH53_12870"
     CDS             1376..1996
                     /gene="ureG"
                     /locus_tag="VHH53_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX2291056.1"
                     /translation="MHREDRTLRIGIGGPVGGGKTALVAALCRTLRDELSLVVVTNDI
                     YTTEDADFLRTNAVLPDERIRAIRTGCCPHTAIRDDITANLDAVEELTARYHPDVVLV
                     ESGGDNLTATFSYGLIDAQVFVLDVAGGDKVPRKGGPGVTRSDLLVINKIDLAPMVGA
                     DLDVMRRDALAVRGDRPTVLISLREQPDAGPVVDWMRSQLQSPAHR"
BASE COUNT          296 a          751 c          810 g          355 t
ORIGIN      
        1 ccggatccgg ccctccacca tcgcggcgga ggacctgctg catgacctcg gggccatttc
       61 gatgatcggc tcggattcgc aagcgatggg ccggatcggc gaggtgatcc tgcgaacctg
      121 gcagaccgcg cacgtcatga agcgccggat gggggcgcta cccggggacc cggcgatatc
      181 ggacaacctg cgggcgcgcc gctacgtggc caagtacacg atctgtccgg ccgtcgccca
      241 cgggctcgac ggtgaggtcg gctcggtgga ggtaggcaag ctcgccgacc tggtgctgtg
      301 gcagcccgcg ttcttcggcg tccgtcccag cgtcgtgctc aagggcggca tgatcgcctg
      361 ggcggccatg ggcgactcca acgcctccat cccgaccccg cagccggtcc tgccccggcc
      421 gatgttcggt gctgctgcaa ccaccgcagc tgctacctcg gtgcacttcg tggctccggc
      481 agcgatacaa gatggcctcg gcgaccggct ggcagtacgg cgcaggctgg tggcggtgcg
      541 cgatactcgg gcgctgcgta aggccgatat gccattgaat gctgcgctgc cgagcatcga
      601 ggtggaccct gggacgttca ccgtccggat cgacggcgag gtggtcgaac cgcacccggt
      661 gcaggagctg ccgatggccc agcgctactt cctgttctga tggtcacgct gggatcgctg
      721 ctgctggccg attctcggtt gcccaccggg gggcacgcgc attccggtgg ggtggaggca
      781 gccgtcgagc gcggtctgct tcagaacgag tccgaccttg cgctgttcct ggcgggccgg
      841 ttgcgcggca gcggcccggt gatcgccgcg gtggctgccg ccggctgcct gctgaccgcc
      901 gacaccggct cgcagtgggg ccgttgggat gcagcggtca gtgcgcgtac cccgtcggcc
      961 gcagtgcggg agtcgtcgcg ggcgcagggc gcggcactgc ttcgcaccgc ccggcgggtc
     1021 tggccgtcag ccgccctgga ggcgctgtct gagatgggcc gcccgcatcg cccgctggtc
     1081 ctcggcgctg tggttgccgc cgtcggggac gggccggacg tggcagcgtt actggcactg
     1141 caccacctgc tcggcggggc gtgcgccgcc gcggtgcggt tgctcgggct ggacccgatc
     1201 gccgttgccg ccgtgcagac gcgcgcggca gagcccgccg agcaggcggc gctggcggcg
     1261 acctggctgg cgcgcgaggc cgtcgtcggg cgagacaccc gcctgctgcc cagtaccggt
     1321 accccgctgc cggaggtgct ggccgaactg cacgcccgca cggaggtgac cctttttgca
     1381 tcgtgaggat cggacgctgc ggatcggcat cggcgggccg gtcggcggcg gcaaaaccgc
     1441 gctggtcgcg gcgctgtgcc gaacactgcg cgacgagctg tcgctggtag tggtgaccaa
     1501 cgacatctac accaccgagg acgccgactt cctgcgcacc aacgcggtgc tgcccgatga
     1561 acggatccgg gccatccgca cgggctgctg cccgcatacc gcgatccgcg acgacatcac
     1621 cgccaacctg gatgcggtgg aggagctcac cgcgcgctat caccctgatg tggttctggt
     1681 ggaaagcggc ggggacaacc tcactgcgac attcagctac ggcttgatcg acgcgcaggt
     1741 gttcgtgctc gacgtcgccg gtggcgacaa ggtgccccgc aagggcgggc ccggtgtcac
     1801 ccgctccgat ctgctggtga tcaacaagat cgatctcgcg ccgatggtcg gcgctgacct
     1861 ggacgtgatg cgccgcgacg ccctagcggt ccggggggat cggcccaccg tgctgatctc
     1921 gctgcgtgag cagcccgacg ccggaccggt cgtggactgg atgcgcagcc agctccagtc
     1981 gcccgcacac cggtgagagc ccgggcgcag cttgcagccg aattcgggcc ggatgaaacc
     2041 cgcctgtggt ggcgttgctc tcctccgatt gcgttgcacc agaccggtcc gcaccgggtg
     2101 cacctggtgc aggcggcagg tggcccgctg ggtggcgacg agatggcgtt ggacatccac
     2161 cttggtggtg gcgcatctct gcaggtgcgc tcggcggcgg ccacggtggt cc
//
LOCUS       DATADN010000998         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_577568, whole genome shotgun sequence.
ACCESSION   DATADN010000998 DATADN010000000
VERSION     DATADN010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577568"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VHH53_12875"
     CDS             complement(<1..602)
                     /locus_tag="VHH53_12875"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2291057.1"
                     /translation="MMLETKRMLVGRPLRTASLSQQLLPKRLALPVFCSDPISSVAYA
                     TQEILLVLTLGGAALVGLAPQVALAIVILLVIVVASYRQICFAYPNGGGAYVVSRDTL
                     GERASLIAASALLVDYVLTVAVSVVAGVDAVTSYAPSLQRHAVALALIFIALLTVVNL
                     RGIKESGRGFAVTTYGFIAGIYLMFAVAAWRVWTGQQLRAV"
     gene            complement(668..841)
                     /locus_tag="VHH53_12880"
     CDS             complement(668..841)
                     /locus_tag="VHH53_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291058.1"
                     /translation="MHRLVSVAKVGGELDDHLVAAAQQPCRGEQGGAVVTVRLPLGDD
                     PVAAGPCLPRLPI"
     gene            complement(865..>1782)
                     /locus_tag="VHH53_12885"
     CDS             complement(865..>1782)
                     /locus_tag="VHH53_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX2291059.1"
                     /translation="PALLRPGRFDRQVVIPLPNQQERKAILTVHSRGKPLGPDVDLQV
                     VARGTPGFSGADLGNLANEAAIFAVRAGRQVIEAQDFAEARDRVLLGRRDSSNALLPQ
                     EKHAVAVHESGHALVAALSEHADPVAKVTILSAGQALGATQQLPIDERHLYSESYLKD
                     SLAIRMGGRAAELLVFGETSTGAANDLAGATDLATRMVREFGMSPALGPVGFASGNPL
                     YLGGEEVKSRPYAEATQRIIDEEVATLLREAEQRATVTLTNHRRALDRLTELLLERET
                     IDGTAVDDILGPIPGHQEPAGATANAMSG"
BASE COUNT          326 a          591 c          583 g          282 t
ORIGIN      
        1 actgcgcgca gttgctgccc ggtccagacc cgccacgccg caaccgcgaa catcaaataa
       61 atcccggcga tgaacccgta agtcgtcacg gcaaaaccgc ggccggattc cttgatcccg
      121 cgcaggttca ccaccgtcag cagcgcaata aaaatcagcg ccagcgcaac cgcgtgacgc
      181 tgcaagcttg gagcgtagga ggtgaccgcg tccacgccag cgacgaccga cactgccacg
      241 gtcaacacgt aatccaccaa caacgcactg gctgcgatca ggctggcccg ctcgccgagg
      301 gtgtcacggc tgacgacata ggcaccgccg ccgttggggt aggcaaagca gatctgccga
      361 tacgacgcga ccacaatcac caacaagatc acgatggcca gcgctacctg cggcgccaat
      421 ccaaccagcg ccgcaccccc cagcgtcagc acgagcagga tctcctgcgt cgcgtaggcg
      481 accgaggaga tcgggtcact gcagaacacc ggcagtgcca acctcttagg cagcagctgc
      541 tggctcaggc tcgcggttcg cagtgggcga ccgacgagca tccgcttggt ctccagcatc
      601 atgggaaccg accatgtcag cggattgatg agcagatgat gagaggcgcc agcgctagac
      661 agccgcgcta gatcggcaac cgtggcaggc aggggccggc cgcgaccggg tcgtcaccca
      721 acggcagccg tactgtcacc accgcgccgc cctgctcacc gcggcacggc tgctgcgccg
      781 cagcaaccag gtggtcgtca agttcgccac cgaccttggc cacgctaacg aggcgatgca
      841 accacagaac ctgccaacga gcgcttagcc gctcattgcg ttggccgttg ccccggccgg
      901 ctcttggtgg cctgggatag ggccgaggat gtcgtctacg gcggtgccgt cgatggtttc
      961 ccgttccagt agcagctcgg tgaggcgatc cagcgcgcgg cggtggttgg tgagcgtgac
     1021 ggtggcacgt tgttcggcct cgcgcagcaa cgtagccacc tcctcgtcga ttattcgctg
     1081 ggtggcctcg gcataggggc ggcttttgac ctcctcgcca cccagataca gggggttgcc
     1141 ggaggcgaac ccgactgggc caagtgccgg actcatgccg aactcgcgca ccatccgggt
     1201 ggccaggtca gtcgcgccgg ccaggtcgtt ggcggcgccg gtggaggttt cgccaaagac
     1261 gagcagttcg gcggcccgcc cgcccatgcg gatggccaac gagtctttca ggtagctctc
     1321 ggagtacagg tggcgttcgt cgatgggcag ctgctgagtg gctccgaggg cttgaccggc
     1381 ggacagaatg gtgaccttgg cgaccggatc tgcgtgttcg gatagcgccg cgaccagggc
     1441 atggccgctt tcgtggacgg ccacggcgtg tttttcctgg ggcagcagtg cattggagct
     1501 gtcccggcgg cccagcagga cccggtccct ggcctcggcg aaatcctgcg cttcgatcac
     1561 ctgccggcca gcgcgtacag cgaagatcgc agcctcgttg gccagattgc ccagatcagc
     1621 accagaaaac cccggcgtgc cccgcgcgac cacttgcagg tccacgtctg ggcccaaggg
     1681 cttacctcgg ctgtggacgg tgagaatggc cttgcgttct tgctggttgg gcagggggat
     1741 caccacctgc cggtcgaagc ggcccgggcg cagcagggca gg
//
LOCUS       DATADN010000999         2246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_262577, whole genome shotgun sequence.
ACCESSION   DATADN010000999 DATADN010000000
VERSION     DATADN010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262577"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..2023)
                     /gene="asnB"
                     /locus_tag="VHH53_12890"
     CDS             complement(77..2023)
                     /gene="asnB"
                     /locus_tag="VHH53_12890"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX2291060.1"
                     /translation="MCGLLGLLTAQRDAPVRVAAVTAALSCQRHRGPDETGTWHDSDV
                     VLGFNRLSIIDIEHSHQPLRWGPPEAPERYTILFNGEIYNYLELRDELQSEPYGVRFV
                     TEGDTEVILAAYHHFGTAAVQRLRGMFAFIIWDAQLRVAFGARDPFGIKPLFSCVGPG
                     GVAFASEKKSLLELAPSLGVSAVSGMSMEVDHRALQLYLSLQYVPEPASLHAGITRIE
                     SGTSFTLSPGGKLITDRYFHPDFRPRPVRSKAESGVLHDKIAMALRDSVAKHMRADVT
                     VGAFLSGGIDSTAIAALAREHNPDLITFTTGFEREGYSEVDVAAELAAAIDVRHVVRT
                     VSAAELAEAVPLIVWYLDDPVADPALVPLWFIAREAREHVKVVLSGEGADELFGGYTI
                     YREPLSLAPFEKVPGALRRSLGRISTRIPQGVRGKDLLRRGSLTLEQRYYGNARIFPD
                     DQLRGVLRGYDPTISHTDVTAQAYAASTGWDPVTRMQHVDLFTWLRGDILVKADKMTM
                     ANSLELRVPFLDTEVFQVAATIPLGEKITRETTKHALRRALRDIVPAHVLNRRKLGFP
                     VPIRHWLKDELYDWARGIVDDSGTAELLNLAAVRRMLDEHRAGLRDHSRRIWAVLVFM
                     LWHGIFVEQRIVPKVPEPHYPVQV"
BASE COUNT          389 a          706 c          758 g          393 t
ORIGIN      
        1 ccttcaactg agcccggcca agtcggcgtg ttgggcgctg gtgcatggtg tgtccggcac
       61 cggtgcccgc gcccggctag acctgtaccg ggtagtgcgg ttcgggcacc ttgggaacga
      121 tccgctgctc gacgaagatg ccgtgccaga gcatgaaaac cagcaccgcc cagatgcgcc
      181 tgctgtgatc ccgtaggccg gcccgatgct cgtcgagcat gcggcgtacc gccgcaaggt
      241 ttagaagctc cgcagtgccg gagtcatcga cgatgccgcg ggcccagtcg tacagctcgt
      301 ccttgagcca gtgccggatc gggaccggga aacccagttt gcgccggttg agaacgtgtg
      361 ctggcacgat gtcgcgcagt gccctgcgta gggcgtgctt ggtggtctcg cgggtgatct
      421 tttccccgag cgggatggtg gcggccacct ggaatacctc ggtatcgagg aagggcaccc
      481 gtagctccag cgagttcgcc atggtcatct tgtcggcctt gaccaagatg tccccacgta
      541 gccaggtgaa caggtccacg tgctgcatgc gggtcaccgg atcccagccg gtcgaggcgg
      601 cgtaggcctg cgcggtgacg tcggtgtgcg agatggtcgg gtcgtatccg cgcagcaccc
      661 cacgcagctg atcgtccggg aagattcgtg cgttgccgta atagcgctgt tccagcgtca
      721 gtgatccgcg gcgtagcagg tccttgccgc gtactccctg tgggatacgg gtggagattc
      781 gtcccagcga tcggcgcaac gcaccaggga ctttctcgaa aggcgccaac gaaagcggtt
      841 cacggtaaat ggtgtatcca ccgaagagct cgtctgcgcc ctcgccggag agcaccacct
      901 tcacgtgttc cctggcctcc ctggcaatga accacagcgg caccagcgcc gggtcagcca
      961 ctggatcatc gaggtaccac acgatcagcg gcaccgcctc ggccagctcg gcggctgaga
     1021 cggtacggac cacgtgccgg acgtcgatcg cggcggccaa ctccgcggcc acgtctacct
     1081 cggagtatcc ctctcgctca aagccggtgg tgaaggtgat caggtccgga ttgtgctccc
     1141 tggccagagc ggcgatggca gtggaatcga tgccgccgga caggaaggcc ccgacggtga
     1201 cgtcggcccg catgtgcttg gcgaccgagt cccgcagcgc catggcgatc ttgtcgtgca
     1261 ggacaccgga ctcggccttg gaacgcaccg ggcgcggccg gaaatcggga tggaagtacc
     1321 gatcggtgat cagcttgcca ccgggggaga gggtgaagga ggtaccggac tcgatccggg
     1381 ttatccccgc gtgcagggac gccggctcgg gtacgtactg caggctcagg tagagctgca
     1441 acgctcgatg gtccacctcc atagacatgc cagacacggc tgacacgccc aacgagggtg
     1501 cgagctccag caggctcttc ttttcactgg cgaaggcgac tccacccggc cccacgcaac
     1561 tgaagagtgg cttgataccg aagggatccc gggcaccgaa tgccacgcgc aactgcgcgt
     1621 cccagatgat gaaagcgaac attcctcgca gccgctgcac cgcagccgtc ccaaagtggt
     1681 ggtaggcggc caggatgacc tcggtgtcgc cttcggtgac aaatctcacg ccgtaaggct
     1741 cgctttggag ttcgtcgcga agctcgaggt agttgtaaat ctctccgttg aacaggattg
     1801 tgtagcgctc aggcgcctct ggtggccccc accgcagggg ctggtgcgag tgctcgatgt
     1861 cgatgatgga cagccggttg aacccgagca ccacgtcgga gtcgtgccag gtcccggtct
     1921 cgtcgggacc gcgatgccgc tggcagctta aggcggcggt cacggccgcc actcggactg
     1981 gcgcgtctcg ctgcgctgtc agcagtccca gtaacccgca cacgcgctca cccttccgtc
     2041 gatggacgac agagtatgac gaccgcttgc gcggaacacc cgctccccgg gggagctgcg
     2101 ggcgcgacgc tggtttaggc tccagctagt gagctgacgg ccgcattggg gcgacgagga
     2161 ggaaccaggc gtgatcggcg tggtccgctc tcgcgcggct cggctagcga aggtggccgg
     2221 tctggccggg atggtcgcct ttgtgt
//
LOCUS       DATADN010001000         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1315528, whole genome shotgun sequence.
ACCESSION   DATADN010001000 DATADN010000000
VERSION     DATADN010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315528"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1490)
                     /locus_tag="VHH53_12895"
     CDS             complement(54..1490)
                     /locus_tag="VHH53_12895"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX2291061.1"
                     /translation="MTEGTSTPTTEAGFVVVANRLPVDLEQLPDGSQRWKSSPGGLVV
                     ALEPFLRSHHGAWVGWAGVPDVEVEPFIDGDITLHPVALSSQEIAEYYEGFSNGTLWP
                     LYHDVVAPPAFHRTWWDAYVRVNERFAAAAAKSAREGATVWVQDYQLQLVPRMLRELR
                     PDLRIGFFLHIPFPPVELFMQLPWRTELVRGLLGADLVGFHLPGGAQNFLYLARRLAG
                     MSVTRSAVGIRTRPGQVDIGDRTVRVGAFPISIDSTALDRLARSKSVRRRAAEIREEL
                     GNPATVLLGVDRLDYTKGIDVRLRALRELYAEDWISADDVVMVQLATPSRERVEHYQR
                     MRKDIEQAVGGINGEFGRVGHPAVHYLHQSIPRSELVAFFVAADVMIVTPLRDGMNLV
                     AKEYVACRTDLSGALVLSEFTGAAVELTSAFLVNPHDLDGIKHAVCQAISVDPEDGRR
                     RMRALRRQVLTYDVERWARSFLGALSDS"
     gene            1599..1671
                     /locus_tag="VHH53_12900"
     tRNA            1599..1671
                     /locus_tag="VHH53_12900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1632..1634,aa:Thr,seq:tgt)
BASE COUNT          347 a          664 c          611 g          316 t
ORIGIN      
        1 gtaacagtgc cataacactg ttacgcactc acgagctcga tagcggggac ggctcaggaa
       61 tcgctgaggg cgccgaggaa cgagcgcgcc cagcgctcaa cgtcgtaggt cagcacctgc
      121 cggcgcagcg cccgcatccg cctgcggcca tcctcagggt caacactgat ggcctggcag
      181 acggcatgct taatgccgtc gaggtcatgg ggattgacca ggaatgcgct agtcaactcg
      241 actgctgcac cggtgaattc ggacagcacc agggcgccgc tcagatcggt ccggcaggcc
      301 acgtactcct tggccaccag gttcatcccg tcccgcagcg gcgtgacgat catgacatcc
      361 gcggccacga agaatgcgac cagctcgctg cgcggtatcg actggtgcag atagtgcacc
      421 gcagggtgtc ctacccggcc gaactcaccg ttgatgccgc cgaccgcctg ctcgatgtcc
      481 ttgcgcatcc gctggtagtg ctccacccgc tcgcgcgacg gggtagccag ctgcaccatg
      541 acgacgtcgt cggccgaaat ccagtcttcc gcatagagct cccgcagcgc gcgcagccgc
      601 acgtcgatgc ccttggtgta atccagccgg tccaccccga gcagcacggt cgccggattg
      661 cccagctcct cgcggatctc ggctgcgcgg cggcgcaccg acttgctgcg ggccagccgg
      721 tccagtgcgg tcgaatcgat ggagatcggg aaagcaccga cccgaacggt acggtcgccg
      781 atgtccacct gcccgggcct ggtgcggatc cccaccgcgc tcctggtcac cgacatcccg
      841 gccagcctgc gtgccaggta caggaagttc tgcgcacccc caggcaggtg gaagccgacc
      901 agatcagcgc cgagcaggcc gcggaccagc tcggtgcgcc agggcagttg catgaacagc
      961 tccaccggcg ggaacgggat gtgcaggaag aacccgatcc gcaggtctgg tcgcagttcc
     1021 cgcagcattc gaggtaccag ttgcagctgg tagtcctgca cccataccgt tgccccctcg
     1081 cgggcactct tggcagcggc ggcggcgaat cgctcgttga cccggacgta ggcatcccac
     1141 cacgttcgat gaaatgcggg gggagccacc acatcgtggt agagcggcca cagcgtcccg
     1201 ttggaaaagc cctcgtagta ctcagcgatc tcctggctgg aaagtgccac cggatgcaag
     1261 gtgatgtcgc cgtcgatgaa gggctccacc tccacgtccg gcacaccggc ccagccgacc
     1321 caagcgccgt ggtgggaacg caggaacggt tccaacgcga cgaccaaccc gcccgggctg
     1381 gacttccacc gctgcgagcc atcgggcagc tgttccaagt ccaccggcag ccgattggcc
     1441 accactacga aacccgcctc ggtggtgggg gtactcgtgc cctcggtcac gggttggcct
     1501 cccttgtcca cgccgtcgtc ctcagagact agcggcgtga cccgtcacct gcctgttgaa
     1561 gatccttccg gacgatgcgc ttaccatgag ctgcacgcgc cggcgtagct caactggcag
     1621 agcatccgcc ttgtaagcgg aaggttcggg gttcgagtcc ccgcgtcggc tccgctctca
     1681 ccagggataa cactccttgt cgagttcttg atcaggtccg cgtgacacca ttcgtgacac
     1741 gaaacgtgct agcgtgcgct tcgtgacgcg atcacagggg cctatcgcgc cccgaagccg
     1801 gggcagcatc gacgagctgc cgagcggggc tttgcgagtc cgcgtctatg ccgggcgtga
     1861 tccggtgagc aagcgccggc atgacctgat cgagatcgtc gctccgggac ccggagctgc
     1921 taagcaggcc cgtcgggt
//
LOCUS       DATADN010001001         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_105937, whole genome shotgun sequence.
ACCESSION   DATADN010001001 DATADN010000000
VERSION     DATADN010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105937"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VHH53_12905"
     CDS             complement(<1..1711)
                     /locus_tag="VHH53_12905"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HEX2291062.1"
                     /translation="MTTDVISPAESGPATAPVTTQSRWHWRNWPVVVKLAAVLLVPTI
                     VALVAGVLRIVDQADAGPGYARISQIVRVQQQLSDLIGAVQRERELATAFVARGRTGD
                     PQPLNAQFQTVDSAAQAVATASDQVPGLQSTGYPAALNQLSALGPLRASVTTGFGPVE
                     VVVTEYGATIDPLIGLDAALTRQLDDVAVTDDAAAGHALIAGREQISRQHAIVSAALY
                     ANRLTPGQIEDVRTAAVRLRIEEDALRGALGPADRADAARVGGEAELSRQRLLQLVLN
                     RGAVGEPLNTSPEEWDRRNAAVIGNIAAVENELRQRIDQTALALRDEVRTSAGLNSVI
                     LFFALALGVLVGVFITRALLRPLGVLKRTALDVADRLPEAMARIRDGDVPEKVIAPVA
                     VSTHEEIGQVARAFDEVHSQAVRLAGEQAQLRANVNDIFVNLSRRTQGLVERQLKLID
                     RLESGEQDPKQLDNLFQLDHLATRMRRNSENLLVLAGTDSANRSGRPVRLVDVLRAAV
                     SEVEQYQRLVLQQPPVVLVLGRAASDLVHLLAELLDNATQVSPPDSHVMISSEFGAEG
                     SVTI"
BASE COUNT          339 a          709 c          688 g          334 t
ORIGIN      
        1 caatcgtgac cgagccttcc gcaccgaatt cgctggagat catcacgtgg ctgtccgggg
       61 gtgagacctg agtcgcgttg tccagcagct cagccaacag gtgcaccagg tcgctcgccg
      121 cccgaccgag cacgagaacg acgggtggct gctgcagcac gagccgctgg tactgctcca
      181 cctcggacac tgctgcgcgc aacacgtcga ccagtcgaac cggccggccg gaccggttag
      241 ccgaatcggt tccggcaaga accaacaggt tctcgctgtt acgccgcatc cgggtcgcaa
      301 ggtggtccag ctggaacagg ttgtccagct gtttgggatc ctgctcaccg ctctccagac
      361 gatcgatgag cttgagctgc cgctcgacca aaccttgggt gcggcgcgac aggttgacga
      421 agatgtcgtt gacgttggcg cgtagctgag cctgctcgcc agccagccgg accgcctgac
      481 tgtgcacctc gtcgaacgcc ctggcaacct gcccgatctc ctcgtgggtg ctgaccgcga
      541 ctggggcgat caccttctcg ggcacgtcac catcacggat tcgggccatc gcttccggca
      601 gccggtcggc aacgtcgagc gccgtgcgtt tgagcacacc gagcggtcgc agcagcgccc
      661 gggtgatgaa cacgccgacc agcacaccca gcgccagcgc gaagaacagg atgactgaat
      721 tgagaccagc cgaagtgcgc acctcgtcac gcagcgctag cgcagtctga tcgatccgct
      781 gccgtagctc attctcgaca gcggcaatat tgccgatcac cgccgcgttg cgcctatccc
      841 attcctcggg cgaggtgttc agcggctcac cgacggcacc gcggttgagt acgagctgca
      901 acagccgctg ccgggacagt tcggcctcac caccgactcg ggcggcgtca gcgcgatcgg
      961 ccggtcccag ggccccccgg agtgcgtcct cttcgatgcg tagccggacg gctgccgtcc
     1021 gcacatcttc gatctggcca ggggtgaggc ggttggcata gagggccgcg gaaacgatag
     1081 cgtgctggcg actgatctgc tcgcgcccag cgatcagcgc gtgcccagcc gcagcgtcat
     1141 cggtgacggc gacatcgtcg agctgacggg tgagcgcggc gtccagcccg atcagcgggt
     1201 cgatcgtggc gccgtactcg gtaaccacaa cttccaccgg accgaaaccg gtcgtcacgc
     1261 tcgcccgcag tggccccagc gcgctgagtt ggttcaacgc ggcggggtaa ccagtggatt
     1321 gcaggcccgg tacctggtcg ctggccgtcg cgaccgcttg tgcagcgctg tccacggtct
     1381 ggaactgcgc gttcagcggc tgcgggtcac cagtacggcc cctggcgacg aacgccgtgg
     1441 cgagctcccg ttcccgctgc acggcaccga tcagatcgga cagctgctgc tgcacgcgga
     1501 cgatctggga gatgcgagcg tagccaggtc cggcgtcggc ctggtccacg attcgcagca
     1561 cacccgcgac cagggcgacg atggtgggca ccagcaggac agcggccaac ttgaccacga
     1621 ccggccagtt gcgccagtgc caccgggatt gggtggtcac cggcgcagtc gccggccccg
     1681 actccgcagg gctgatcacg tcggtggtca tgacgccgcc tccgcaggct gtgacgatgg
     1741 cgcctcagcg gcaaagcgtg cttcggcgcg ccgtgcatcg gcggtcatga tgccgcctcc
     1801 gcgggatgaa gtgcgtcggc gatcagacca gcctcccgcg gcggccgaga gtcgcgagct
     1861 agtcgctggc acagtggggt ccctccccag ggtttgacgt tggccggcag gcgcatccgg
     1921 ctggcgccac ggttcgccgc gatcgtgggc ttccgcaccg cacggacggg gcacctctgg
     1981 caccgggccg gatacgtccc ttgtagggca cggacgatac gacgacctgg gcagggcctt
     2041 cccgcgggtc tcgtagaact tcctccctgc
//
LOCUS       DATADN010001002         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1184784, whole genome shotgun sequence.
ACCESSION   DATADN010001002 DATADN010000000
VERSION     DATADN010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184784"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..325)
                     /locus_tag="VHH53_12910"
     tRNA            complement(243..325)
                     /locus_tag="VHH53_12910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(290..292),aa:Leu,seq:cag)
     gene            467..>1652
                     /locus_tag="VHH53_12915"
     CDS             467..>1652
                     /locus_tag="VHH53_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX2291063.1"
                     /translation="MGKAQRFERRLQGMVGDAFARVFGGSVVPQEVAQALQREADDNA
                     RPLAGGRVLAPNRYIVTLGATDHDRLAGDEQRVIRMLKSCIQSHLAVQSWDVYGDVVV
                     ALERSETQHTGQFRTSSSVDPDAPQPRTAGDRAMSQQQGEPPEHDHRGQYAPHRGSGH
                     DQRQGDANLNEGQPYGYDFPYHQDQGHPQSHGDHNHGSQNYGVQNYGSPGGYDHNYHQ
                     YGYDQGRNDEQGYGQGQGGYGYEPGYGQRQQAPGEGYPQTYGDPNYGQYGGAQPGYGY
                     EHGGYGADQDYGADHDYRTQSYVPPAVQTGSRQLAASLILDDGSNRHYQLTEGATVVG
                     RGQEAQFRLPDTGVSRRHLEITWDGWTATLADLGSTNGTTVNGSPVQTWQLADGDVVR
                     VGH"
BASE COUNT          319 a          548 c          553 g          232 t
ORIGIN      
        1 gatcgggtca gcggcgatct cgataccggc cagtcgcttc ccctcacgat gccagtcctg
       61 cgcgaagacg taagtcatgt cccctctctc aagccgtccg tgctgctgca ccgaccgcag
      121 ggcagggtga atctgaccgt caggcccgat cgtcgcacac gttgctgatg atctgtgggc
      181 agcgagcagc ccgcgatgcg ggtgaggccc agccccgctc tggcctgccc aacgaagagg
      241 agtgggcgag gggggagttg aacccccacg tccttacgga cacacggacc tgaaccgtgc
      301 gcgtctgcca ttccgccact cgcccgagtg atcgcggaag cttagcatcc agccttcgaa
      361 gggccggacg cagtagaccc tgctcgctgg ccgtcccgga tacgatcggg ggagactggc
      421 aggaaactgg cagcgaatcc tggtagagcc tggaaaggag ggcgccgtgg gcaaggcgca
      481 gcggttcgag cgccggctgc aaggtatggt cggcgacgcc tttgcccggg tcttcggcgg
      541 tagcgtggtg ccgcaggagg ttgcccaggc actccagcgt gaagccgacg acaatgcgcg
      601 tccgctcgcg ggggggcggg tgctcgcgcc caaccgttat atcgtcacac tcggggcgac
      661 cgaccacgac cggctcgcag gcgacgagca gcgggtaatc cggatgctca agagctgcat
      721 ccaaagccac ctcgctgtgc agtcctggga tgtatacgga gacgtggtgg tggctctaga
      781 gcggtcagag acgcagcaca cgggacagtt ccgcaccagc tcgtccgttg atcccgacgc
      841 accgcaacct cgtaccgcag gagaccgagc gatgagccag caacagggcg agccccccga
      901 gcacgaccac cggggtcagt acgccccgca ccgcggttct ggccacgacc agaggcaggg
      961 tgacgccaac ctcaacgagg gccagccata cggctatgac ttcccctacc accaggacca
     1021 gggccatccc cagagccatg gcgaccacaa ccatggcagc cagaactacg gcgtccagaa
     1081 ctacggcagt cccggcggct acgaccacaa ctatcaccag tacggttatg accagggtcg
     1141 caacgacgag cagggctacg gccaaggcca gggtggctat ggctatgagc ccggttacgg
     1201 gcagcgtcag caggcccccg gcgagggcta cccgcagacc tacggagatc ccaactacgg
     1261 ccagtacggc ggtgcccagc cggggtacgg atatgagcac ggtggctacg gggctgatca
     1321 ggactacggg gctgaccacg actaccgcac ccagagctac gtgcctccgg cggtgcagac
     1381 cggttctcgc cagctggcgg cctcgctcat cctggacgac gggtcgaacc gtcactatca
     1441 gctcaccgag ggcgccacgg tggtgggccg tggccaagag gcgcagttcc ggctcccgga
     1501 caccggcgtg tcacggcggc acttggagat cacctgggac ggctggacag cgacgcttgc
     1561 cgacctcggg tccaccaacg gcacgacggt caacggcagc ccggtacaaa cgtggcagct
     1621 cgccgacggg gacgtggtgc gggttgggca ct
//
LOCUS       DATADN010001003         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_431669, whole genome shotgun sequence.
ACCESSION   DATADN010001003 DATADN010000000
VERSION     DATADN010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431669"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VHH53_12920"
     CDS             complement(<1..355)
                     /locus_tag="VHH53_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291064.1"
                     /translation="MLLTLIRRRWTVLATIVVVAIPALVACGGSGAGEGGDTGNANTI
                     EVNTAGIEFADRLVVYNGRSHYGDEQVFRDFEDRTGVKIELRGGTAPELFERLRQEGA
                     DTPADLLITTDLANLW"
     gene            465..1499
                     /locus_tag="VHH53_12925"
     CDS             465..1499
                     /locus_tag="VHH53_12925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2291065.1"
                     /translation="MSPFAIHAMDIRKAFGSSHAVAGATLQVKQGELVALLGPSGSGK
                     TTLLRVIAGFEVPDTGSVWIGGRQVAGEGVWEEPDRRRIGMVFQDGALFPHLTIAGNL
                     AFAGPRPGRAQECLELVGLASRAASYPHELSGGERQRIALARALAADPEVVLLDEPFA
                     ALDEGLRVSLREEVATIIRTAGASALLVTHNQQEALSLADVVAVMRDGRIEQVGPPEG
                     VYSRPVTRWVAEFLGDADVVPGTAAGGVAACELGRFPTDRALDGMVEIIVRPEGVTIG
                     PDGADAPADGAVEARVVGRSYFGHDQLVQLELASGLRLRSRIPGSAAWRSGDPVRVRV
                     TGPVTVLGPS"
BASE COUNT          234 a          517 c          569 g          266 t
ORIGIN      
        1 gccacaggtt ggccagatcg gtggtaatga gcaggtcagc gggggtgtcc gcaccctcct
       61 gccgcagccg ctcgaacagc tcgggcgcgg taccaccacg caactcgatc ttgactccgg
      121 tcctgtcctc gaagtcgcgg aagacctgct cgtcgccgta gtgtgaacgg ccgttgtaga
      181 cgacgagccg atcggcgaac tcgatgccgg cagtgttgac ctcgatggtg ttcgcgttcc
      241 cggtgtcacc gccctcgccc gccccgctac caccgcaggc gaccaaggcg gggattgcca
      301 cgacgacgat ggtggccagc acggtccacc ggcgacggat gagagtcaag agcatcgctg
      361 tcctccgggc atgttcgcgt cagggctcac agccgctgac ggcccccgag ccgccgacca
      421 tatataccta tatgtcgcat attttagggt agcttaacca cagtatgagc ccgttcgcga
      481 tccatgccat ggatatccgt aaggccttcg gatcaagcca cgctgtcgcc ggggcgaccc
      541 tccaggtgaa gcagggggag ctggtggccc tgctgggtcc gagcgggtcg gggaagacga
      601 cactgctgcg cgtcatcgcc ggtttcgagg tacccgacac cggaagtgtg tggatcggcg
      661 ggcggcaggt ggcgggcgag ggcgtctggg aggagcctga ccggcgccgg atcgggatgg
      721 tttttcaaga cggcgcgctg ttcccgcacc tgaccatcgc cggcaacctg gccttcgccg
      781 gaccgcggcc gggccgcgcg caggagtgtc tcgagctggt gggtctggcg tcgcgggccg
      841 cgtcgtaccc acacgagctc tcgggcggcg agcggcagcg gatcgctctg gcccgcgcgc
      901 tggcggccga ccccgaggtg gtgctgctcg acgagccgtt cgccgcactg gacgaggggt
      961 tgcgggtcag cctgcgtgag gaggtcgcca ccatcatccg gaccgcggga gcgagcgccc
     1021 tgctggtcac acacaaccag caggaggcgt tgtcgctggc cgacgtcgtg gccgtgatgc
     1081 gggatggccg catcgagcag gtcggccccc cggagggggt ctacagccgc ccggtgacgc
     1141 ggtgggtggc cgagttcctc ggtgacgccg atgtcgtgcc gggcactgcc gccggaggcg
     1201 tcgccgcctg cgagctggga cggtttccca ccgaccgtgc actggatggc atggtcgaga
     1261 tcatcgtgcg accggagggg gtgaccatcg gtccggacgg cgcggacgcc cctgccgatg
     1321 gcgctgtcga ggcgcgcgtc gtaggtcgtt cttacttcgg tcacgaccag ctcgtgcagc
     1381 tggagctggc cagcgggctg cgcttgcgca gccgcatccc tggttccgcg gcctggcgtt
     1441 ccggtgaccc cgtccgagtg cgggtgacgg ggccggtgac cgtgctcgga ccctcctagt
     1501 tacttcggct tgaaaagtgg acgcgttcgc ccagacctgc cagcgcctcg tcggccccac
     1561 cggagtgata agaagctgta actctc
//
LOCUS       DATADN010001004         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_578157, whole genome shotgun sequence.
ACCESSION   DATADN010001004 DATADN010000000
VERSION     DATADN010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578157"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..536
                     /locus_tag="VHH53_12930"
     CDS             351..536
                     /locus_tag="VHH53_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291066.1"
                     /translation="MGVGGPYVANRLSPIRSFRNIGNGYFAGILVPVVIMIVVYLVIW
                     FVLERTPVRQAGVRRRR"
     gene            complement(961..1200)
                     /locus_tag="VHH53_12935"
     CDS             complement(961..1200)
                     /locus_tag="VHH53_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291067.1"
                     /translation="MAEVLRMAGTDLVWELDDSIDLASRAKEIVTARASNQFVTLMAR
                     LPGQRELAYVTLAPGQLAWWSLGEESDPSAMGWGQ"
     gene            1647..>2018
                     /locus_tag="VHH53_12940"
     CDS             1647..>2018
                     /locus_tag="VHH53_12940"
                     /inference="COORDINATES: protein motif:HMM:NF039955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5994 family protein"
                     /protein_id="HEX2291068.1"
                     /translation="MGVEDTATRSALAPADGADGGSVRLALIDPPAVRTTLDGVWWPR
                     TRDLTQELPALVEELDRRGIRVARVAYNPDLRGPAPQRLDADGRTIRLGWFRNLDRQL
                     LNMTGDSSRGRLDFLVVPADTT"
BASE COUNT          302 a          728 c          655 g          333 t
ORIGIN      
        1 atcgaacccc taacccccgc cttgcaaaac cgaccgaggg gtggtttggc acagtgggct
       61 gtgactgaaa acccgtcctg agcagcactt tcaccattgg ccgtcgtcga gcggtgttgg
      121 ccgttttcgg ccgtttggcg gactatctgc ggactgggcc gccacagcag tcgcctcgga
      181 cactgccctg acgacagcac ccgcccccac acccgcagga gcctcaccga ctgcgccggc
      241 cgccggaagg gcgctgcgca tccctgcggg atgggcctac ggcccaccct tcccccggcc
      301 cgctgcagcc ggatcaaagc cggttgtggg cagcggccag gagtgtgtgg atgggcgtcg
      361 gaggccccta cgtcgccaac cgattgagtc caatacgttc cttccggaac atcggaaacg
      421 gctacttcgc cggcatactc gtgcccgtcg tcatcatgat cgtcgtctac ctggtcatct
      481 ggttcgtgct ggagcgcacc ccggttcggc aagcaggtgt acgccgtcgg cggtaaggcc
      541 gaggctgccc ggctggccgg gatcaatgtc cgcgcgtcac cacggccgtc cacgtgattg
      601 ccggtgcgtg cgcgggactg gccggcatca tcttcgccgc ctcgagatct cggcccagcc
      661 gacgggcggc atcggctacg agatcgacac gatcgccgcg gtcgtgcttg gtgggacggg
      721 ccttttcggc ggtcggggct ggctatacgg cgccctgatc ggctcggtga tcctgggtgt
      781 gctcagcacc ggcctgatcc tgatgaacgt ccagttgttc acccagctgc tgattccaca
      841 gcctgaagcg ccgctgccgg tcaacctctg ggcaccgctg ccgggtcgcc tgggcttgcc
      901 cgtcctgcgg cgtcctcgac ggggtcccca tgtcgagccg gcgttcggac tctcaggaac
      961 tcactggccc caccccatcg cgctcggatc gctctcctcg ccgagtgacc accaggcgag
     1021 ctgtccgggt gcaagggtca cgtaggcgag ctcgcgctgg cctggcagcc gcgccatcag
     1081 cgtcacgaac tggttgctcg cccgggccgt cacgatctcc ttggcgcgac ttgcgagatc
     1141 gatggagtcg tccagttccc acacgaggtc cgtcccggcc atcctcagca cctctgccat
     1201 gtctcccagc acatcactcg tgcatccgga tgagccaccc gaccagcgcg gcacactgcc
     1261 gctgccggca ccatgcgccc gatctggctg cgcccagctt ccgggacact cagcagccgc
     1321 tcggtggtcc ggcagcgacg agcaccgtgc cgggcttcga cgatccgccg cagggcgcga
     1381 cgcaccgcat caccgcgaac catccgtccg ccccggaagg gtccgaatgc ccgtggcatg
     1441 cgaactgacg gcagctcgac cggtcccggg gacccggatg agcgcaagtt gaccagtgac
     1501 gccagttcgg cgtcatgccc ctccgctacc ggagggagat cggcggtccc tccgttgcgg
     1561 ctcacgagca cacagacgtc tcctcgactc ggaaccgtgc ggtcgtgacg accatccgag
     1621 tcacccgccg accgcggaga ccctcggtgg gcgttgagga cacagcgacg cggtcagcac
     1681 tcgcaccggc cgatggcgcc gacgggggct cggtccgcct cgctctaatc gacccaccgg
     1741 ccgtacgtac cacgttggac ggcgtgtggt ggccccggac acgggacctg acccaggagt
     1801 tgcctgcttt ggtcgaggag ctggaccgtc gggggatcag ggtcgcgcgg gtggcttaca
     1861 accccgacct ccgggggccc gcgccgcagc ggctggacgc ggacggccgg accatccgcc
     1921 tcggctggtt ccgaaacctc gaccggcagc tgctgaacat gaccggtgac tcgagccgcg
     1981 gccggctgga cttcctcgtg gttcccgcgg acacgacg
//
LOCUS       DATADN010001005         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_578195, whole genome shotgun sequence.
ACCESSION   DATADN010001005 DATADN010000000
VERSION     DATADN010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578195"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /gene="leuD"
                     /locus_tag="VHH53_12945"
     CDS             complement(<1..399)
                     /gene="leuD"
                     /locus_tag="VHH53_12945"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX2291069.1"
                     /translation="MEPFTRHTGVGVPLRRSDVDTDQIIPAVYLKRVSRTGFEDGLFA
                     AWRAHEDFVLNHEAYRCGSVLVAGPDFGTGSSREHAVWALMNYGFRVVISPRFGDIFR
                     GNAGKQGLLAAQCERADVELLWKLLETEPGT"
     gene            complement(416..1837)
                     /gene="leuC"
                     /locus_tag="VHH53_12950"
     CDS             complement(416..1837)
                     /gene="leuC"
                     /locus_tag="VHH53_12950"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX2291070.1"
                     /translation="MGRTLAAKVWETHVICRGEGNGNSAAPDLLYIDLHLVHEVTSPQ
                     AFDGLRLAGRPVRRPDLTIATEDHNVPTMDIELPIADEVSRTQVDTLRRNCAEFGVRL
                     HPMGDVEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLAT
                     QTLPLRPFNTMSVVVRSRDGSLPAGVTSKDIILAVIAQIGTGGGQGYVLEYRGNVIEQ
                     LSMEARMTVCNMSIEAGARAGMIAPDATTFDYLAGRPHAPVGPKWDTAVQYWRSLHTD
                     DDATFDAEIHIDADTLTPFVTWGTNPGQGLPLSERVPDPEQITDEGERLAAAKALSYM
                     DLIPGTPLREIAVDTVFLGSCTNGRIEDLRAAAVLAGRRVRNGVRMLVVPGSMRVRAQ
                     AEAEGLHEVFTAAGAQWRSAGCSMCLGMNPDQLAAGERCASTSNRNFEGRQGKGGRTH
                     LVSPLVAAATAVRGTLSAPADLD"
     gene            1906..>2497
                     /locus_tag="VHH53_12955"
     CDS             1906..>2497
                     /locus_tag="VHH53_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX2291071.1"
                     /translation="MGQHSGIGVLDKAMTVLRAVAANPCGLAELCSRTGLPRATAHRL
                     AVGLEVHRLLRRDSDGRWRPGPALHELANGALDPLLDAAAMVLPRLRDLTGESVQLYR
                     RDGMIRVCVAAAEPPSGLRDTVPVGARLPMTAGSGAKVLAAWADPATQRAVLGEAVFG
                     ERILMEVRRRGWAQSVAERESGVASVSAPVRDAAGSV"
BASE COUNT          403 a          828 c          851 g          415 t
ORIGIN      
        1 ggtgcccggc tcggtctcca gcagcttcca gagcaattcg acgtcggctc gctcgcactg
       61 ggctgcaagc aagccttgct tgcccgcgtt gccccggaag atgtcgccga accgtggcga
      121 gatcaccacc cggaacccgt agttcatcaa cgcccacacc gcatgctcac gcgatgatcc
      181 agtgccgaag tcgggacctg cgaccagcac gctgccgcag cggtaggcct cgtggttgag
      241 cacgaagtcc tcgtgggcac gccacgccgc gaacagcccg tcctcgaaac cggtgcggct
      301 gacccttttg aggtaaacgg cagggataat ctgatcagta tccacatcgg agcgacgcag
      361 cgggacacca acaccggtgt gacgggtaaa cggttccatt gctgctcctc acgactcaat
      421 cgaggtcggc gggtgctgac agggtgcctc ggacggcggt ggcggccgcg accagggggg
      481 agaccaggtg ggtgcggccg cctttgccct ggcgaccctc gaagttgcgg ttggaggttg
      541 acgcgcagcg ctccccggca gcgagctgat cggggttcat ccccaggcac atcgagcatc
      601 cggccgagcg ccactgagcc ccggccgcag tgaacacctc gtgcaggccc tcggcctcgg
      661 cctgggctcg cacccgcatc gagccgggaa cgaccagcat tcgcacgccg ttgcgcaccc
      721 tgcggccggc gagcaccgcc gccgcccgca ggtcctcgat ccgtccgttg gtacaggacc
      781 cgaggaacac ggtatccacc gcgatctcgc gcagcggggt accaggtatc aagtccatat
      841 aagacagtgc cttggcagct gcgagccgct cgccttcgtc ggtgatctgc tcgggatcgg
      901 gcacccgctc ggataacggt aggccctggc cggggttggt gccccaggtg acgaacggcg
      961 tcagggtgtc ggcgtcaatg tgtatctcgg cgtcgaaggt ggcgtcgtcg tcggtgtgca
     1021 gtgaacgcca gtactgcaca gccgtgtccc atttcggccc cacgggcgca tgcggacggc
     1081 cggccaggta gtcgaacgtg gtggcatccg gcgcgatcat gcccgcccgg gcacccgcct
     1141 cgatcgacat gttgcagacc gtcatccgag cttccatgga cagctgctcg atgacgtttc
     1201 cgcgatactc cagcacatag ccttggccac cgccggtacc gatctgggcg atcacggcca
     1261 ggatgatgtc cttgctcgtg acgcctgcgg gcagcgaccc atcgcggctg cgcacaacca
     1321 ccgacatggt gttgaacggg cgcagcggca gggtctgagt ggccagcacg tgctccacct
     1381 cggaggtgcc gataccgaac gcaagcgcac cgaaggcacc atgggtagag gtgtgggagt
     1441 caccgcagac cacggtcatc cccggctggg tgagacccag ttgaggtccg acgacatgca
     1501 cgatgccctg ttcgacgtcg cccatcggat gcagccgtac gccgaactcc gcgcagttcc
     1561 ggcgcagcgt gtccacctgg gtgcgggaca cctcgtcggc gatcggcaac tcgatgtcca
     1621 tggtgggcac gttgtgatcc tcggttgcga tcgtcagatc tggccggcgg accggccgac
     1681 ccgccagccg cagcccatcg aatgcctgcg ggctggtgac ttcatgcacc agatgcaggt
     1741 cgatgtagag caggtcggga gccgcgctgt tgccgttgcc ctcaccgcgg catatcacgt
     1801 gggtctccca gaccttcgct gcgagcgtgc gtcccatcgc ctggttcctc cgctaagctt
     1861 ctcacatgct ggacttccca tatcgcggga agttagtatc ggtctgtggg acagcatagc
     1921 ggcatcggcg tgctggacaa ggcgatgacg gtactgcggg cggtagcggc gaacccgtgc
     1981 ggcctcgccg agctgtgttc ccgtacaggt ttaccccggg ccaccgcgca ccggctggcg
     2041 gtggggctgg aagtgcaccg gctgttgcgt cgcgacagcg acggacggtg gcgtcccgga
     2101 cccgccctgc acgagctggc taacggtgct ctcgatccat tgctcgacgc cgcggcgatg
     2161 gtgctgcccc ggctgcgtga cctcaccgga gaaagcgtcc agctctaccg gcgggacggg
     2221 atgatccggg tatgtgtggc agctgccgaa cctcccagcg ggctgcgtga caccgtcccg
     2281 gtgggagccc ggctgccgat gaccgccgga tcgggagcca aggtcttggc tgcgtgggcc
     2341 gatccggcca cccaacgtgc tgtgctcggc gaagccgtgt tcggcgagcg aatcctgatg
     2401 gaggtgcgcc ggcggggctg ggcgcagagc gtggccgagc gcgagtccgg agtggccagc
     2461 gtgtccgccc cggtgcgcga tgcggccggc agcgtgg
//
LOCUS       DATADN010001006         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1034437, whole genome shotgun sequence.
ACCESSION   DATADN010001006 DATADN010000000
VERSION     DATADN010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034437"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1114
                     /gene="ruvB"
                     /locus_tag="VHH53_12960"
     CDS             74..1114
                     /gene="ruvB"
                     /locus_tag="VHH53_12960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX2291072.1"
                     /translation="MTTESPLSAEVDPADVELETTLRPRRLPEFIGQARVREQLELVL
                     EGARRRGTAPDHVLLSGPPGLGKTSLAMIIAAELGSAIRITSGPALERAGDLAAMLSN
                     LVEGDVLFIDEIHRVARPAEEMLYLAMEDFRVDVVVGKGPGATSIPLEIAPFTLVGAT
                     TRSGALTSPLRDRFGFTAHMEFYEPEELELVLRRSAGILGVDLRPDGATEIAGRSRGT
                     PRIANRLLRRVRDYAEVRADGAITLEVARAGLAVYDVDELGLDRLDRAVLTALVRTFG
                     GGPVGVSTLAVAVGEEPATVEEVCEPFLVRAGMLARTPRGRVATAAAWRHLQLQPPPD
                     TPGDATPTLFDG"
     gene            1211..1546
                     /gene="yajC"
                     /locus_tag="VHH53_12965"
     CDS             1211..1546
                     /gene="yajC"
                     /locus_tag="VHH53_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX2291073.1"
                     /translation="MDPLLLPLLFIAVLALPLILGARRQKRAMAEAQRLQSSLTDGDR
                     VMTTSGLHGTVVGSADETTIDLEIAAGVRTTWLRAAIREKITEDDEAAATSAPVAAPI
                     EDGTRTDKP"
     gene            1683..>2147
                     /locus_tag="VHH53_12970"
     CDS             1683..>2147
                     /locus_tag="VHH53_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX2291074.1"
                     /translation="MAPPAVHFRPWRNLAIFAGIVAVLYSLVFFTGGGDPTPKLGIDL
                     QGGTRVTLTARTVDGQPPSRESLDQARQIIEQRVNGIGVSGAEVVLDGNNLIITVPGE
                     NGDQARSLGQTAKLYFRPVITEVPASLAGPTPGGQAPSGQNPGSQAPSGQAPE"
BASE COUNT          341 a          722 c          734 g          350 t
ORIGIN      
        1 acgggcagtc tcgtgacacg tctgacaccc cggggatcct gcgcagcgca ctgacccggc
       61 tggggcgcaa ccggtgacta ccgagtcgcc gctgtcggct gaggtagacc cggcggacgt
      121 cgagctggaa accacgctgc gaccacgccg gctgcccgag ttcatcggtc aggctcgggt
      181 gcgtgagcag ctcgaactgg tgctggaagg cgcccggcgg cgcggtaccg ccccggacca
      241 cgtgctgctg tccggcccgc cggggctggg aaagacgagc ctcgcgatga ttatcgctgc
      301 ggagctgggc agcgcgatcc ggatcacctc ggggccggcg ttggaacgcg ccggcgatct
      361 cgccgcgatg ctgtccaacc tcgttgaggg tgacgtgctg ttcatcgacg agatccaccg
      421 ggtggctcgg cccgccgagg agatgctcta tctcgcgatg gaggattttc gggtcgacgt
      481 ggtggtcggc aagggtcccg gagccacctc gatcccactg gagatcgccc ctttcacttt
      541 agtcggagcc actacacgtt ctggtgcgct gaccagtcca ttgcgcgatc ggttcgggtt
      601 caccgcgcac atggagttct acgaacccga ggagctggag ctggtcttgc ggcgctcggc
      661 cggcatcctc ggtgtggacc tgcgccccga cggcgccacc gagatcgccg gccgctcccg
      721 cggcacgcct cggatcgcga accggttact gcgccgggta cgtgactacg ccgaggtccg
      781 ggccgacggt gcgatcacgc tcgaggtggc ccgtgccggc ttggctgtct atgacgtcga
      841 cgagcttggg ctggatcggc tggatcgggc tgtgctcacc gcgctggtac gcaccttcgg
      901 tggcggaccg gttggcgtgt ccacactcgc cgttgcggtc ggtgaggagc cggccaccgt
      961 ggaggaggtg tgcgagccct tcctggtccg tgccggaatg cttgctcgca ctccccgtgg
     1021 ccgggtagct accgcagccg cgtggcggca cctgcaactg cagccgcctc cggacactcc
     1081 cggagacgcc accccgacgc tgttcgacgg atgagacctg cttgcagggt cgaaccatcc
     1141 acaccgagac ctgcttgcag gagcgacctg gcacactcgg gcgtgcaaca ccgatggtga
     1201 tcggagaacc atggaccccc tgctcctgcc cctgcttttc atcgccgtgc tggcattgcc
     1261 cctgatcctc ggcgcacgcc ggcagaagcg ggcgatggcc gaggcgcagc ggctgcagag
     1321 ctcgctgacc gacggtgacc gggtgatgac tacctccggc ctgcacggca ccgtggtcgg
     1381 cagcgctgac gagaccacga tcgatctgga gatcgccgct ggagtgcgca ccacgtggct
     1441 gcgcgcggcg atccgagaaa agatcaccga ggacgacgag gcggcagcca caagcgcgcc
     1501 ggtggcagcc cccatcgagg acggcactcg caccgacaag ccgtgacctg accggtgtgg
     1561 gtacgctgcg cgtccggccc ccaccgccgg tgccgatgac ggcgctggca cagtgtgggc
     1621 cgcggcaggt tgccgtgatg gcgcccggcg cagtgagctg ttaacgagga gaaccaccca
     1681 ccgtggcacc accggccgtg cacttccgcc cctggcggaa cttggcgatc ttcgctggaa
     1741 ttgtcgctgt cctgtattcg ctggttttct tcaccggcgg gggagatccc actcccaaat
     1801 taggcatcga ccttcagggc ggcaccaggg tgaccctcac tgcccgcacc gtggacggcc
     1861 agccgccgtc gcgggaatcg ttggatcaag ctcgtcagat catcgagcag cgggtcaacg
     1921 gcatcggggt gagcggggca gaggtcgtcc tggacggcaa caacctgatc atcacggtcc
     1981 ctggtgaaaa tggtgatcaa gcccgcagcc tcggtcagac cgccaagctg tacttccgac
     2041 cggtcatcac cgaggtgcca gccagtctcg ctgggccgac acccggcggt caggctccga
     2101 gcggtcaaaa cccaggcagc caggctccga gcggtcaggc tcctgag
//
LOCUS       DATADN010001007         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_431833, whole genome shotgun sequence.
ACCESSION   DATADN010001007 DATADN010000000
VERSION     DATADN010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431833"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VHH53_12975"
     CDS             <1..1304
                     /locus_tag="VHH53_12975"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX2291075.1"
                     /translation="IFLIGSALSGMAQDMEQLIGFRAIQGLGAGGLMVGVMAIIGDLI
                     PPRERGRYQGMMAGVMAIAMIGGPLVGGTITDHWGWRWSFYINLPLGVVALAAISVVL
                     HLPKKRSEARIDYLGAALLTVGITAIVLVTTWGGTEYAWSSARIMELIGIGVAALVGF
                     VFWQTKAAEPVVPLHIFRNRNFTLMSVIGFITGFVMFGATLFLPLYQQSVQGASATNS
                     GLLLLPMLGAMLVTSMVAGRVTTNTGRYKVFPILGSVLMTVGLYLLSTMDTGTSRFTS
                     GVFMAVVGFGMGCLMQITMLVAQNSVEMKDMGVASSSTTLFRTLGSSFGVAIMGALFN
                     NRVQDVMAERAGALGSKVTERSAQLDAKGLEKLPVVVREAYQHAVSAGTHSAFLLGAV
                     VAVVALVAAVFVREVPLRGAGPKTEDKPDTPAAQPPVVEAV"
     gene            1442..>1575
                     /locus_tag="VHH53_12980"
     CDS             1442..>1575
                     /locus_tag="VHH53_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2291076.1"
                     /translation="MDRDWADPDLDLGAVAAHAGYSRYHFIRAFKEAYGETPGQYLTH
                     R"
BASE COUNT          220 a          529 c          553 g          273 t
ORIGIN      
        1 tcatcttcct gatcggctcc gcactgagcg gcatggccca ggacatggag cagctgatcg
       61 ggttccgcgc catccagggg ctgggtgcgg gcgggctcat ggtcggtgtt atggcgatca
      121 tcggcgacct cattccgccc cgggagcgcg gcaggtacca gggcatgatg gccggcgtga
      181 tggccatcgc gatgatcggc ggaccgctgg ttggcggcac catcaccgac cactggggct
      241 ggcgctggtc cttctacatc aatctgccgc tcggtgtggt cgcgctcgct gcgatcagcg
      301 tcgtcctgca cctgccgaag aagcggtccg aggcgcggat cgactacctg ggcgccgcgc
      361 tgctgaccgt cggcatcacc gcgatcgtgc tcgtcaccac ctggggcggc acggagtacg
      421 cctggtcctc cgcgcggatc atggagctga tcggcatcgg ggtcgccgcg ctcgtcgggt
      481 tcgtgttctg gcagaccaag gccgccgagc cggtcgtgcc gctgcacatc ttccgcaacc
      541 gcaacttcac gctgatgtcc gtcatcggct tcatcaccgg cttcgtgatg ttcggcgcga
      601 cgctcttcct gccgctgtac cagcagtccg tgcagggtgc ctccgcgacg aactccgggc
      661 tgctgctcct gccgatgctc ggcgcgatgc tggtgacctc gatggtcgcc ggacgggtca
      721 ccacgaacac cggccggtac aaggtgttcc cgattctcgg cagcgtcctg atgaccgtcg
      781 gtctctacct cctgtccacg atggacacgg ggacctcgcg gttcacgtcc ggtgtcttca
      841 tggccgtggt cgggttcggc atgggctgcc tgatgcagat cacgatgctg gtcgcgcaga
      901 acagcgtgga gatgaaggac atgggcgtcg cgtcctcgtc caccaccctc ttccgcaccc
      961 tcggctcctc cttcggcgtc gcgatcatgg gcgcgctgtt caacaaccgc gtccaggacg
     1021 tcatggccga gcgggccggg gcgctgggct ccaaggtgac cgagcggtcc gcgcagctgg
     1081 acgcgaaggg cctggagaag ctgccggtgg tggtgcggga ggcgtatcag cacgcggtgt
     1141 ccgccggcac gcactcggcg ttcctgctcg gggccgtggt ggcggtggtc gcgctggtgg
     1201 cggccgtgtt cgtgagggag gtgccgctca ggggagcggg gcccaagacg gaggacaagc
     1261 cggacacccc ggcggcgcag ccgcccgtgg tcgaggccgt ctgaccggca cgcacgaagg
     1321 cccccgggtg tccacccggg ggctctgccg tgcctggccg cacacgtcct gattgtcaga
     1381 cccccgtgcc accatcgatc gcatggacaa gatgcggcag gtgcggctgg ccaaggacgc
     1441 catggaccgg gactgggccg atccggacct ggacctgggc gccgtggcgg cacacgccgg
     1501 gtactcgcgg tatcacttca tccgcgcctt caaggaggcg tacggcgaga cccccgggca
     1561 gtacctcacg caccg
//
LOCUS       DATADN010001008         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_263365, whole genome shotgun sequence.
ACCESSION   DATADN010001008 DATADN010000000
VERSION     DATADN010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263365"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VHH53_12985"
     CDS             <1..321
                     /locus_tag="VHH53_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX2291077.1"
                     /translation="RWLEEARDRIHAELSHRPTLSRLAQEVGVHPVTLARAFRQSFGC
                     TVGEYVRHLRVERAAHQLAGTELSLAEIALAAGFSDQSHFSNMFREHTGLSPSKFRRV
                     VRPA"
     gene            391..1467
                     /locus_tag="VHH53_12990"
     CDS             391..1467
                     /locus_tag="VHH53_12990"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX2291078.1"
                     /translation="MSIHRTTLTAVLGALAIALPLAGQTVLDRSPNLAGAWTGSSGTL
                     HFNFLHRFQASSEPERKVTSTPTFLIAAGLPWRTLVGAHYATNSELSPRYPNEWEFFG
                     RIAVLDQESGAPLDASAQIGYNLAADQPDAEVALARRQGPVRVLAAGRVLGAPDNVGG
                     ADVALGAGAVFRLTRHIALSADLTSLVERGPGEKVAWGAGVNLAIPRTPHTLSLHATN
                     VNNASLQSSSRGTEETRYGFEFTIPLTLSRYFGRSPRPSQAGPQQPAQEMGDTANSTG
                     ATLSARVEDFSFVPARLEVQRGTRVTWTNRGQVIHTVTAIDGSFDSGNIDSGATASIT
                     FSRPGTYQYHCTPHPFMRGVIVVR"
BASE COUNT          257 a          536 c          512 g          243 t
ORIGIN      
        1 cgctggctgg aggaagcgcg cgaccggatt cacgccgagc tgtcgcatcg cccgaccctg
       61 agcaggctgg cccaggaggt cggagtgcac ccggtcaccc tcgcacgcgc gttccggcag
      121 tcgttcggct gcaccgtcgg cgagtacgtg cggcatcttc gggtcgagcg cgcggcccac
      181 cagcttgccg ggaccgagct gtcgctcgcc gagattgcgc tggccgccgg gttctcggac
      241 cagagccact tctcgaacat gttccgggag cacaccggat tgtccccatc gaagttcagg
      301 cgggtggtga ggccggcctg accagcttac ctgctccaac cccgttgcta ctttccaaga
      361 cccagggtca gccccacaat atctagagga atgtcaatcc accgtacgac gctcacggcg
      421 gtgctcggcg cgctcgcaat agcgttgccc ctagccggac agacggttct ggaccggagc
      481 ccgaacctgg ccggcgcctg gaccggctcg tcgggcacgc tgcacttcaa cttcctgcac
      541 cggttccagg ccagctcgga gccggagcgg aaggtgacca gcaccccgac gttcctcatc
      601 gcggctggtc tcccctggcg cacgctggtc ggcgcgcact acgcgaccaa ctcggagctg
      661 tcgccccgct accccaacga gtgggagttc ttcggccgga tcgcggtcct ggaccaggag
      721 tccggcgcac cgctcgacgc ctcggcccag atcggctaca accttgcggc cgaccaaccc
      781 gacgccgagg tggcgctggc acgacgacag ggcccggttc gagtactggc agcgggaagg
      841 gtgttgggag cgccggacaa cgtgggcgga gcggacgtgg ccctgggagc cggtgcggtg
      901 ttccgcttga ctcgccacat agccctgtca gccgacctga cctcgctggt cgagcgcggg
      961 ccgggcgaaa aggtggcatg gggcgccggc gtgaacctcg cgattccccg gacgccacac
     1021 acgctctcgc ttcacgcgac caacgtgaac aacgcatcgc tccagagctc gtcgcgcggc
     1081 accgaggaga cccgctacgg gttcgagttc accatcccgc tcacgttgag ccgctacttc
     1141 ggcaggagcc ctcgtccctc ccaggccggg ccacagcagc cagctcagga aatgggagac
     1201 acagccaatt ccaccggggc gacgctctcc gcccgagtgg aagacttcag cttcgtgccc
     1261 gcacggctcg aggtgcagcg ggggacgcgg gtgacctgga ccaaccgggg ccaggtgatc
     1321 cacacggtga ccgcgattga cgggagcttc gactcgggga acatcgattc gggtgccaca
     1381 gcgagcatca cgttctcgcg accgggcacg taccagtatc actgcacgcc gcatccgttc
     1441 atgcgcgggg tgatcgttgt tcggtgagag ggactatgcg ctcccggcta tgcgctgtgc
     1501 ataggaccag aatagtcccc tgcccctcgt cgggctggat ggcggggg
//
LOCUS       DATADN010001009         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1034905, whole genome shotgun sequence.
ACCESSION   DATADN010001009 DATADN010000000
VERSION     DATADN010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034905"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..722)
                     /locus_tag="VHH53_12995"
     CDS             complement(39..722)
                     /locus_tag="VHH53_12995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX2291079.1"
                     /translation="MPADRLTVPADPGPFGCDPARTALVLIDMQRDFVEPGGFGETLG
                     NDVSLLQAVIPPLREVLAATRAAGMTVIHTREGHRGDLADCPPAKLNRGNPTLRIGDV
                     GPKGRILIRGEYGHDIVDELYPVDGEIVIDKPGKGSFYATDLHQLLQTVGIGSLIVTG
                     VTTEVCVHTTVREANDRGYECLVLSDCVGSYFPEFHAVGLRMIAAQGGIFGWVARSED
                     LLCALGSDG"
     gene            complement(712..1470)
                     /locus_tag="VHH53_13000"
     CDS             complement(712..1470)
                     /locus_tag="VHH53_13000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX2291080.1"
                     /translation="MTRTLSRLNLLGPIAADPYPWPYDGTVAAGRTALICIDWQVDFC
                     GRGGYVDRMGYDIELTRAGLPGAAQMLAHARSIGMLVIHTREGHAPDLSDLPTNKRWR
                     SAQIGAEIGGPGPQGRILVRGEPGWEIVDEVAPLPGEVLIDKPGKGAFYATPLDLVLR
                     TKGITHIVLTGITTDVCVHTTMREANDRGYECLILSDCTGATELGNHTAALRMVTMQG
                     GVFGCVATSEAVLAAITVDITTVDITTVGDHHAG"
     gene            complement(1467..>1667)
                     /locus_tag="VHH53_13005"
     CDS             complement(1467..>1667)
                     /locus_tag="VHH53_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291081.1"
                     /translation="ARPAHDIARSTIGKMRYVTVLPDVNALINAIVVRGPQTVYAAEQ
                     REPCRVVDLVLERIASTRKRTL"
BASE COUNT          267 a          548 c          570 g          282 t
ORIGIN      
        1 gaaggcgttg aggtcgccgc gggtccacag ggggatggtc atccgtcgct cccgagtgcg
       61 cacagcaggt cctccgaccg ggcgacccag ccgaagatgc cgccctgggc ggcgatcatc
      121 cggagcccca ccgcgtggaa ctccgggaag taggagccga cgcagtccga gagcaccagg
      181 cactcgtagc cgcggtcgtt ggcctcccgg accgtggtgt gcacgcacac ctcggtggtg
      241 acgccggtga cgatcaggct gccgataccg acggtttgga gcagttggtg caggtcggtg
      301 gcgtaaaacg aaccctttcc cggcttgtcg atcacaatct cgccgtccac cgggtaaagc
      361 tcgtcgacga tgtcgtggcc gtattcgcca cggatcagga tccggccctt ggggccgaca
      421 tcgccgatcc gcagtgtcgg attgccgcgg ttgagcttgg ccggcgggca gtcggccagg
      481 tcgccgcggt gaccctcacg ggtatgaatg acggtcatcc cggccgcccg ggtggccgcc
      541 agcacctccc gcagcggcgg gatcacggct tgtagcaggc tcacgtcgtt gcccaacgtc
      601 tcgccgaaac cccctggttc gacgaagtcc cgctgcatgt cgatgagcac cagggcggtc
      661 cgcgcggggt cgcagccgaa cgggccggga tcggccggca cggtcagccg gtcagccggc
      721 atggtggtct cccacagtgg tgatatccac agtggtgata tccacagtga tggcggccag
      781 caccgcctcg gacgtggcga cgcagccgaa gaccccgccc tgcatggtca ccatccgcag
      841 cgccgcggtg tggttgccca gttcggtggc cccggtgcag tcggagagga tcaggcactc
      901 gtagccccgg tcgttggcct cacgcatggt ggtgtgcacg cagacgtcgg tggtgatccc
      961 ggtcagcacg atgtgggtga tgcccttggt gcgcagcacc aagtcgagtg gggttgcgta
     1021 gaaagcgccc ttacccggct tgtcgatcag gacctcacct ggcagtggcg ccacctcgtc
     1081 cacgatttcc cagcccggtt cgccgcgaac caggatacgg ccctgtggcc cggggccgcc
     1141 gatctccgcg ccgatctgtg ccgagcgcca tcgcttgttg gtcggtagat cggacaggtc
     1201 cggggcgtga ccctccctgg tgtggatcac cagcatcccg atggacctgg cgtgggccag
     1261 catctgcgca gctccgggca ggccggcccg ggtcagctcg atgtcgtagc ccatccggtc
     1321 gacgtagccg ccgcggccac agaagtcgac ctgccagtcg atgcagatca aggcggtccg
     1381 gccagccgcc acggtgccgt cataaggcca tgggtacggg tcggcggcga tcggcccgag
     1441 cagattgagc cgcgagaggg tgcgggtcat agcgtcctct tgcgggtcga cgcgatccgc
     1501 tccaggacga gatcgactac ccgacacggc tcccgctgct ccgctgcgta gacggtttgc
     1561 ggtccgcgaa ccacgatcgc gttaatcagg gcgttcacgt caggtaaaac cgttacataa
     1621 cgcattttgc cgattgtcga ccgagcgatg tcatgcgctg gtcgagc
//
LOCUS       DATADN010001010         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_725056, whole genome shotgun sequence.
ACCESSION   DATADN010001010 DATADN010000000
VERSION     DATADN010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725056"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VHH53_13010"
     CDS             complement(<1..570)
                     /locus_tag="VHH53_13010"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX2291082.1"
                     /translation="MNVASPTPDVSAVPDAGGRKLLRLEVRNSQTPIERKPPWIRTRA
                     TMGPSFRELKGLVRREGLHTVCEAAGCPNIYECWEDREATFLIGGDQCTRRCDFCQID
                     TGRPTDLDRDEPRRVAESVQSMGLHYSTVTGVARDDLPDGGAWLYAETVRQIHVLNPG
                     TGVELLIPDFNARTELLHEVFSAVPQVLAH"
     gene            complement(581..1510)
                     /locus_tag="VHH53_13015"
     CDS             complement(581..1510)
                     /locus_tag="VHH53_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX2291083.1"
                     /translation="MSRWSAADIPDQTGRTVLVTGANSGLGLQTAIMLAEHGATVLLA
                     CRDAQRGRAALARVRSSSGGTDATLIQLDLADLSSIAKAADEVRSITGDQLDVLVNNA
                     GVMAAPPRRTVDGFELQIGTNHLGHAALTWLLGPALTPGARVVTVSSLAHRGGGLDLD
                     DLNVTRRRYSPGAAYSASKLANLLFTFELDRRARDTGRDLVAVAAHPGLTDTELVFNT
                     ARMHSAGPLAHVARWGNKLIAQGAAAGVLPQLYAATAPDVRGGEYFGPARLGETRGTP
                     VRAAASAAACNAHTARLLWERTAELTGVKPDPA"
     gene            complement(1520..>1771)
                     /locus_tag="VHH53_13020"
     CDS             complement(1520..>1771)
                     /locus_tag="VHH53_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate-protein ligase B"
                     /protein_id="HEX2291084.1"
                     /translation="GVTMHGFALNCQADLAAFDRIVPCGIADAGVTSLTAELGRPVPV
                     AQVQPVVRRAVLDALDGALPVSADELPGRTELAPPVVIR"
BASE COUNT          284 a          605 c          609 g          273 t
ORIGIN      
        1 gtgcgccagc acctggggga cggcgctgaa cacctcgtgg agcagctcgg tgcgggcatt
       61 gaaatcaggg atgagcagct cgactccggt gccgggattg agcacgtgga tctgccggac
      121 ggtctcagcg tagagccagg cgccgccgtc aggaagatca tcacgggcca cgccggtgac
      181 cgttgaatag tgcagcccca tcgactgcac cgattcggcg acccgccgcg gctcgtcacg
      241 gtccagatcc gtggggcggc cggtgtcgat ctggcagaag tcgcagcggc gagtgcactg
      301 atcgccgccg atgagaaacg ttgcttcccg atcctcccag cactcgtaaa tgttgggaca
      361 accagctgcc tcgcagactg tgtgcaggcc ctcgcgccgg accagcccct tgagttcccg
      421 aaacgacggc cccatggtgg cccgggtccg gatccagggc ggcttgcgct cgatgggcgt
      481 ttgactgttg cggacctcca gccgcagcag cttgcggcca ccggcgtcag gtactgcgct
      541 gacgtcaggt gtgggggagg cgacgttcac cggtccgagc ctacgcgggg tccggcttca
      601 cgccggtgag ctcggcggtg cgttcccaca gcagccgcgc ggtgtgcgcg ttgcaggcgg
      661 cggcgcttgc tgccgcccgt accggtgttc cacgggtctc gccgagcctg gccggaccga
      721 agtactcccc gccgcgcacg tcgggtgcgg tggcggcata cagctgcggc aacacacctg
      781 cggcggcccc ctgagcgatc agcttgttac cccaccgcgc aacgtgagcc aacggcccag
      841 cggaatgcat ccgagccgtg ttgaacacca gttcggtgtc ggtgagaccg ggatgggcag
      901 cgaccgcaac gaggtcgcgc ccggtgtcgc gagcccgccg gtccagctcg aaggtgaaca
      961 gcaggttcgc cagcttcgat gcgctgtagg ccgcacccgg gctgtaacgc cgccgcgtga
     1021 cgttcaggtc gtccagatcg agcccgccgc cgcggtgcgc gaggctggag accgtgacca
     1081 cccgcgctcc aggggtgagt gcaggcccga gcaaccacgt cagcgccgcg tgcccgaggt
     1141 gattggtgcc gatctgcaac tcgaagccat cgacggttcg ccgcggtgga gcggccatca
     1201 caccggcatt gttcaccagc acgtcgagct gatccccggt gatcgagcgg acctcgtcgg
     1261 cggccttggc gatcgagctc aggtcagcca gatcgagttg gatcaacgtg gcgtccgtcc
     1321 cgccggaact cgaccgtacc cgggccagcg ctgcccgacc gcgttgcgca tcgcggcacg
     1381 ccagcaacac cgttgcgccg tgttctgcga gcatgatggc ggtctgcaag cccaggccgg
     1441 agttggctcc ggtgaccagc accgtgcggc cggtctggtc ggggatgtcg gctgcgctcc
     1501 atcggctcat cagtcgtgcc tatcggatca ccactggcgg cgccaactca gtgcggccgg
     1561 gtagctcatc agcgctcacg ggaagtgcgc catccagagc atcgaggacg gcgcggcgta
     1621 ccaccggctg cacctgcgcc accggaaccg ggcgaccgag ctcggcggtc agcgatgtca
     1681 ccccggcgtc ggcgataccg cacggcacga tccgatcgaa cgcagccagg tcagcctggc
     1741 agttcaaggc gaacccgtgc atggtcaccc c
//
LOCUS       DATADN010001011         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_725170, whole genome shotgun sequence.
ACCESSION   DATADN010001011 DATADN010000000
VERSION     DATADN010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725170"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VHH53_13025"
     CDS             <1..254
                     /locus_tag="VHH53_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX2291085.1"
                     /translation="ALWSDLVELMHDGVCRGRIDTVRPEHDPIAMGRPARQDRHGGEV
                     YVYRRAGAPCLVCATDVVTARHAGRNLYWCPRCQRRPVV"
     gene            302..635
                     /locus_tag="VHH53_13030"
                     /pseudo
     CDS             302..635
                     /locus_tag="VHH53_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418223.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
     gene            complement(684..1568)
                     /locus_tag="VHH53_13035"
     CDS             complement(684..1568)
                     /locus_tag="VHH53_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2291086.1"
                     /translation="MDLTSDATSSGVFLGQDRRLRLTWRILLAWLGFFGCVLIIGGPL
                     QTATARLPTLISHAAAGLGITISALCLVMLLRRHADRRPWSGIGLTLDRATIPRLLLG
                     LVLAAIVTTVAAAATVQLGLADWDPSANITNELVEQGLASTIIMIAISTLLVQAFPEE
                     LVFRGYMFTNLGETLPLWTTVASSSLIFGSMHVLSNSEATTLGERVLFAVAATGLGLM
                     LAACRTVSGTLWLGIGFHGGYNAFNGRFITVHQDAFIPAWLIVLGILIAGTALTITVR
                     QWRAPLDWRAAPRDAAHR"
BASE COUNT          286 a          508 c          548 g          265 t
ORIGIN      
        1 cggcactgtg gtcagatctc gtcgagttga tgcacgacgg ggtgtgtcgt ggccggattg
       61 atacggtccg cccagagcat gacccgattg cgatgggacg acctgcgcgt caggatcggc
      121 atggcggtga ggtctatgtc taccgccggg ccggtgcgcc gtgcctggtg tgcgctaccg
      181 acgtcgtcac agcacggcac gccgggcgta acctctattg gtgtcctcgc tgccagcgtc
      241 ggccggtggt ttaaggcagc atttgcatgt tctcccacgt taagtagtgt gtactaatcc
      301 agtactgatc ggcgtcggca acgcctaccg cgccgaggtg ctgttccgcc atcagctgga
      361 tccattgcaa ccgggttgcg agctgcgagc tgcgcgctga gcagtggtcg gcgctgtggt
      421 cagatctcgt cgagctgatg aacgaggggg tacgccgcgg ccggatcgat accgtgcggc
      481 cagaacacga tcccgggcgt accgggcgtg ccccacggca ggatcgccac ggcggcgagg
      541 tctacgtcta ccgccgtgcc ggcgccccct gcctggtgtg cggcacgacc gtcgtcacta
      601 tgcggcacac gggccgcaac ctctactggt gtcctgctgc cagcgacggc cagcgtctta
      661 ataccgaggc tgagccgctg ccatcatcga tgagcggcgt cgcggggggc cgcccgccag
      721 tccagtgggg cgcgccactg ccggaccgtg atggtcagcg ccgtcccggc gatcaagatt
      781 ccaagcacga tcagccacgc cgggatgaag gcgtcctggt ggaccgtgat gaagcgcccg
      841 ttaaacgcgt tgtatccgcc gtggaagccg atgccaagcc agagcgtgcc gctgaccgtc
      901 cggcaggccg cgagcatcag cccaagcccg gtcgctgcca ccgcgaagag cacgcgttcg
      961 ccgagggtgg tggcctcgct gttggacaac acgtgcatcg acccgaagat cagggaactg
     1021 ctggccaccg tggtccacag cggcagcgtc tcaccaaggt tggtgaacat gtagccgcgg
     1081 aacaccaatt cctccgggaa tgcctgcacc aagagggtgg atatggcgat catgatgatg
     1141 gtggacgcga gtccctgctc gaccaattcg ttcgtgatgt tggccgacgg gtcccagtcg
     1201 gcgagcccga gttgcacggt ggctgcggcg gcgactgtgg tgacaatcgc cgcgagcacg
     1261 aggccgagca gcaggcgcgg gatcgttgcg cggtccaagg tgagaccgat gcccgaccac
     1321 ggccgccggt cggcgtgccg ccgcagcagc atcaccaggc aaagcgcgct gatcgtgatg
     1381 cccaagccgg cggccgcgtg cgagatcagc gtgggcagcc gggcagttgc tgtctggagg
     1441 ggcccgccga tgatcagcac gcaaccaaag aatcccagcc aggccagcag aatccgccag
     1501 gtcaaccgca gtcggcggtc ctggccgagg aacactcctg atgatgtggc gtccgacgtg
     1561 aggtccatac cgaacataga agaaacatta cgcaccgtgg tcacccg
//
LOCUS       DATADN010001012         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_865505, whole genome shotgun sequence.
ACCESSION   DATADN010001012 DATADN010000000
VERSION     DATADN010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865505"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VHH53_13040"
     CDS             complement(<1..1745)
                     /locus_tag="VHH53_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2291087.1"
                     /translation="MGATEAAGGTRPVDARNRVLVRVATTGLVAVLVTVTGGAAWAAA
                     EVDAAVQEIEQISGQSDAWVQADREVSREESLAHAYLAEPSGDLRLALREADRSATVA
                     LDYLTVHGEPDDAASARQIKAKHAQTVRDLGRMLAAVDAGDAALAKAIHERPVHPAYA
                     TLQHKIRAFADHEQEEVTERLADLRRRQHDLRLASLAAAGVGLFALGVFLLLVVGYQR
                     RLVSQALHDPLTGLPNRELLADRIEQAIRTADRELRPAALLLLDLDRFKDVNDTLGHH
                     HGDQLLVQVSQRLGRALRGVDTVARLGGDEFAVLLPGATADGAGAVADKLRTALQQPL
                     SIGGVALDLDASIGIAIYPEHGRDAAELLQHADVAMYLAKQAHVGFLVYDPAVDQHSP
                     KRLALLGGLRQALDRGDLVLHYQPKADLGSGQIHSAEALVRWQHPEHGLLGPGEFIPL
                     AERTGLIHPLTRWVLDAALAQAARWRHAGLGLSVAVNVSTRCLLDPAFPDQVASQLAT
                     WQVPPGLLMLEITESAVMADPDRALEVLGRLHTLGVGLAVDDFGTGYSSMAYLKELPI
                     DELKIDRSFVSQMAT"
     gene            complement(2011..2409)
                     /locus_tag="VHH53_13045"
     CDS             complement(2011..2409)
                     /locus_tag="VHH53_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291088.1"
                     /translation="MIKKRGNRWWSWPDGGNTPTRREAGPTAAPAIHSKLGGPAGRQG
                     ALVDRQLGQALARHVLHHQVMLVGIGAAVDPGHDGLAQPGQGPGLPPEPSTNDGSWAE
                     GGLSTLTATCRSSTRPVAGCTPDHPAHPQQ"
     gene            2728..3468
                     /locus_tag="VHH53_13050"
     CDS             2728..3468
                     /locus_tag="VHH53_13050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2291089.1"
                     /translation="MSYRPTRSIEGTVAAITGASAGIGAATARALVGAGAKVALGARR
                     NDRLASLVGELGDEHAVALPGDVRDPQYNEALVAAAVEKWGRLDTVVANAGIGAYGGI
                     LDIDDAQLREMVETNYLATVWTVRSGVRQFRAQGDGGDIVIVSSVAGFRGGADEAVYA
                     GTKFAQVGLGGSLDRELRAEGMRVTLICPAGTATEFAIGAGRDEGSPELDAYLRPGDV
                     AYAIRVVLEQPRSVRTTVWQLWSSQQQS"
     gene            3715..4530
                     /locus_tag="VHH53_13055"
     CDS             3715..4530
                     /locus_tag="VHH53_13055"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2291090.1"
                     /translation="MDGSIDPALHEHYALGKERDRLTEARGVLEFERTKEIVLRQLPA
                     PPSEVADIGGGPGRYALWLAERGHRVEHHDLMPLHVEQLRAVADPSRINAAVGDARDL
                     DLGDGAVDAVLLLGPLYHLRRREERLPALREAWRVVRPGGPVFVAAISRWAPRLDGEL
                     RARMYESLPAFRELTPGVERTGWLPPLHPGSFSAYCHRPRQLRAELHAAGFRVLDLVA
                     VEGVGFMLHDLDERLAYPLDRAVVLDAARATERVPELLGIGPHLLATAIRPER"
     gene            complement(4753..>5504)
                     /locus_tag="VHH53_13060"
     CDS             complement(4753..>5504)
                     /locus_tag="VHH53_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2291091.1"
                     /translation="SQLDLALEAGVSARHVSFVETGRARPSPEMVLHLAEQLEIPLRD
                     RNGLLLAAGYAPVYEERDLDAPDMAPIRTALDLVLRGHEPHPAAVVDRSWELIQGNRG
                     IGLLLEGVDPDLLAPPVNVLRVSLHPEGMAPRILNLGEWRAHLLGRLRRQVALTADRG
                     LAELHEELRAYPCDQPEPEVEVPGPGEVVVPLRLRHGGRELSLLSIMASFGTPLDVTV
                     AELAIESFFPADPETGRILRGGPSGVPSGST"
BASE COUNT          759 a         1881 c         2048 g          816 t
ORIGIN      
        1 ctggttgcca tctggctgac gaaggaccgg tcgatcttca gctcgtcaat cggtagctcc
       61 ttcaggtacg ccatggagga gtaaccggtg ccgaagtcgt ccacggccag gcccaccccc
      121 agagtgtgca gccgacccag aacctctagg gcgcggtcgg gatcggccat gacggcgctc
      181 tcggtgatct ccagcatcag caggcccggg gggacctgcc aggtggccag ctggctggcg
      241 acctggtcgg ggaaggccgg gtcgagcagg cagcgggtcg agacgttcac cgcgaccgac
      301 aggcccaggc cggcgtggcg ccatcgggcg gcctgggcca gggcggcgtc cagcacccag
      361 cgggtcaggg ggtggatgag tcccgtccgt tcggccagcg ggatgaactc gcccgggccg
      421 agcaggccgt gttcggggtg ctgccaacgc accagcgcct ccgcgctgtg gatctggcct
      481 gaaccgaggt cggctttggg ctggtagtgc aggaccaggt ccccacggtc caacgcctgc
      541 cgtagcccgc cgagcagcgc gagccgcttg gggctgtgct ggtcgacggc cgggtcgtag
      601 accaggaacc cgacatgggc ctgcttggcc aggtacatgg ccacgtcggc gtgctggagc
      661 agctcggcag cgtcgcggcc gtgctctggg tagattgcga tcccgatgct ggcgtccagg
      721 tcgagggcta ccccaccgat gctgaggggc tgctgcagcg ccgtacgcag cttgtcggcc
      781 accgcaccgg ccccgtcagc ggttgcgccc ggcaacagga ccgcgaactc gtcgccgccc
      841 agccgggcga cggtgtcaac tcctcggagg gcccggccca gccgctggct gacctggacg
      901 aggagctggt cgccgtgatg gtggccgagg gtgtcgttga cgtccttgaa ccggtccagg
      961 tccaacagca acagcgccgc cgggcgcagc tcccggtcgg cggtccggat ggcctgctcg
     1021 attcggtcgg cgagcagctc ccggttgggc aggccggtga gcgggtcgtg cagggcctgg
     1081 ctgaccagcc gccgctggta gccgacaacc agcagcagga acacgccaag ggcgaacaag
     1141 cccactccgg ccgcggccag actggccagc cgcaggtcgt gctggcggcg ccgcaggtcg
     1201 gccaggcgct cggtcacctc ctcttgctcg tggtcggcga acgcccggat cttgtgctgc
     1261 aaggtggcgt aggccggatg gaccggccgt tcgtggatgg ccttggcgag cgcagcgtcg
     1321 ccggcgtcca ccgcggccag catccgcccc aggtcgcgga cggtctgggc gtgcttggcc
     1381 ttgatctgcc gggccgaggc cgcgtcgtcc ggctcgccgt ggacggtcag atagtccagc
     1441 gcgacggtgg ccgaccggtc ggcctcccga agggcgagcc gcaggtcacc ggagggctcg
     1501 gccaggtagg cgtgcgcgag cgactcttcc cggcttacct cgcggtctgc ctggacccag
     1561 gcgtcggact ggccactgat ctgttcgatt tcctggacgg ccgcatcaac ctcggccgcg
     1621 gcccaggcgg cgccgcccgt gacggtgacc agcaccgcta ccaggccggt ggtggccacc
     1681 cggaccagca cacggttccg ggcatccacc ggcctggtcc caccggccgc ttcggtagct
     1741 cccatgacta tggtcccttt ccggggtcac ccgaagccgg agccgcaccg acgcgcgagt
     1801 cgccctcggg tacgtgtgaa ctatcggtgc acgaccagcg aagttgatct ggttacccga
     1861 gagccggcgc cgatttgcat cgcctgcctg gcagggcccg ctgctggtgg ggcgaggtgg
     1921 gtgtcgttgg tggtggctgg tggctgcccg gtagggccgg gcgtgatgga cctggaggga
     1981 cgggcccttg acggccgcta ccagctggcc tcactgctgg gggtgggcgg gatggtcagg
     2041 ggtgcacccg gccaccggtc gggtgctgga gcggcaggtg gcggtcaagg tgctcagccc
     2101 gccctccgcc caggacccgt cgttcgtgga gggttccggc gggaggcccg ggccctggcc
     2161 aggctgagcc agcccatcat ggccggggtc gacagcggct ccgatgccaa cgagcatgac
     2221 ctggtggtgg aggacgtggc gggccagagc ctggcccagc tgccggtcga ccagggcccc
     2281 ctggcgcccc gccgggccgc cgagcttgct gtggattgcc ggtgctgcgg tgggaccggc
     2341 ctcccgccgg gtcggggtgt tgccaccgtc tggccacgac caccagcggt tgccgcgctt
     2401 cttgatcacc aggaggctcc tcgatcaccc gctccgaggg gcaaggatga tgaggcaagc
     2461 aaagcaaaca gcccccaggc cgctgccctg gggcgttggg cgcgccgcga gggattcgaa
     2521 cccccgatcg ccagatccgt gcctgatgct ctgcgtcgag ctggtcggct ccagaccgat
     2581 gcagcgcccg gacacgccgt caggttcatg gagaaccacg gcgagcccga gccgctgtca
     2641 catgggttgg gcgtggcgcc tgcgcgtccg tgcttcatac tgggcgtcca tgcttcctgg
     2701 cgtccacgcc actctggggg tgctcccttg tcgtaccgcc caacccgctc gattgagggc
     2761 accgttgcag cgatcaccgg agcgagcgcc ggaatcggtg cggcgacagc ccgagcgctg
     2821 gtcggggccg gcgccaaggt ggcgttgggc gcccgccgca acgaccgctt ggcttcgctg
     2881 gtgggtgagc ttggcgacga gcacgccgtc gccctccccg gggacgtccg cgacccccaa
     2941 tacaacgagg ccctcgtggc cgcggcggtc gagaagtggg ggcggctgga caccgtggtt
     3001 gccaacgcgg gcatcggcgc ctacggcggc attctggata ttgacgacgc gcagctgcgg
     3061 gagatggtgg agaccaacta tctggccacg gtgtggacgg tgcggtcggg tgtgcggcaa
     3121 ttccgggcac agggcgatgg cggcgacatc gtgatcgtca gctcggtcgc cggcttccgt
     3181 ggcggcgcgg acgaggcggt ctatgcgggc accaagttcg cccaggtggg gcttggcggc
     3241 tcgctggacc gtgagctccg ggcggaaggg atgcgagtga cgctgatctg cccggctggg
     3301 accgccaccg agttcgccat cggtgccggc cgggacgaag gctcgccgga gctggatgcg
     3361 tacctgcgtc ccggcgacgt cgcgtacgcg atccgcgtcg tgctcgaaca accccgctcg
     3421 gtaaggacca ccgtgtggca gctgtggagc agccagcagc aaagctgaca gagtcggttt
     3481 ctcagatgcg caccagtgcg cgcccggctg cagggccggc ccaatcgtcc gtcttccaca
     3541 attttgctcg tcgggggccg acgaaggagg cccatcgatg ggcgctcaat cagccagacg
     3601 gactcctacg tagcgaagcg gggcggagcc cagtgctcgt gcagatacca gcgctggttg
     3661 gtgcatgcag aacaatcagc aaaggtccgg tgcggtgtgc catcgtggcg gttgatggac
     3721 ggttcgatcg accctgccct ccacgagcac tacgccctcg gcaaggagcg cgaccgcctc
     3781 accgaggcgc ggggcgtcct ggagttcgag cgcaccaagg agatcgtgct gcgccagctc
     3841 ccggccccgc cctcggaggt ggccgacatc ggcgggggac ccgggcgcta tgcgctgtgg
     3901 ctggccgagc gcggccaccg ggtcgagcac cacgacctca tgccgctgca tgtcgagcag
     3961 ctccgggcgg tcgccgaccc ctccaggatc aacgctgcgg tcggggacgc ccgggacctg
     4021 gacctgggcg acggcgcggt ggacgccgtg ttgctgctgg ggccgctcta ccatctccgc
     4081 cgtcgcgagg agcggctgcc ggcgctccgg gaggcctggc gggtggtccg cccgggcggt
     4141 ccggtgttcg tggccgccat ctcccgctgg gcgccacggc tggacggcga gctgcgggcg
     4201 cgaatgtatg agtcactgcc ggcattccgt gagctaaccc cgggggtgga gcgtacgggc
     4261 tggctgccgc cgctgcaccc agggtcgttc tcggcctact gccaccgtcc ccggcagctc
     4321 cgggccgagc tgcacgccgc cgggttccgg gtgctggacc tggtcgccgt ggagggcgtg
     4381 gggttcatgc tgcatgacct ggacgagcgg ctggcctatc cgctggaccg cgccgtggtg
     4441 ctggacgccg cccgcgccac cgaacgggtc ccggagctgc ttgggatcgg accgcatctc
     4501 cttgcgaccg cgatccgtcc ggagcgctga tcggccgccg atcgggtcag gcacccgaac
     4561 ctcgcgagag gcgtggtgct atctcgccgt ggcatggcac ggctcgactc gatcctgaac
     4621 ccgccagtct cccgatgcgc caccgcagga ggcgcgacag aaggagaacc gatgaccagc
     4681 gggccagcgg ttggcattcc cacctcggcc cgattcaccg tgcgcccctc ggctggcccg
     4741 gtcgcgccta gactaggttg acccgctcgg aacacccgag gggccgccgc gcaggatgcg
     4801 gccggtctcg gggtcggcgg ggaagaagga ctcgatggcc agctcggcca cggtcacgtc
     4861 cagcggcgtc ccgaagctgg ccatgatgct cagcagcgac agctcccggc cgccgtggcg
     4921 cagccgcagc ggcaccacca cctcgccggg ccccgggacc tcgacctccg gttcgggctg
     4981 gtcgcagggg taggcgcgca gctcctcgtg cagctcggcc aggcctcggt cggcggtgag
     5041 ggcgacctgg cggcgcagcc ggcccagcag gtgggcgcgc cactcgccca ggttcaggat
     5101 ccgcggggcc atgccctcag gatgcagact cacgcgcagc acgttgaccg ggggcgccag
     5161 gaggtccggg tcgacgccct cgagcaggag cccgatcccg cggttgccct ggatgagctc
     5221 ccagctgcgg tcgacgaccg ccgccggatg cggctcgtgg ccgcgcagga cgaggtcgag
     5281 cgccgtgcgg atgggcgcca tgtcgggcgc atcgaggtcg cgctcctcgt acaccggcgc
     5341 gtatccggcg gcgagcagca ggccgttgcg gtcgcgcagc gggatctcga gctgctcggc
     5401 gaggtgcagc accatctccg gactcgggcg cgcgcggccg gtctccacga agctcacgtg
     5461 gcgggcggag acgccggcct ccagcgccag gtcgagctgg ctca
//
LOCUS       DATADN010001013         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1485709, whole genome shotgun sequence.
ACCESSION   DATADN010001013 DATADN010000000
VERSION     DATADN010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485709"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1108
                     /locus_tag="VHH53_13065"
     CDS             395..1108
                     /locus_tag="VHH53_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX2291092.1"
                     /translation="MRRCPAQVSAPRSGFAGFRFPPEVITWAVRWYLRFGLSYRDVEE
                     LLAERGIEVDHVTIYRWVQRFTPLLIDAARPCRHVPGDRWFVDETYVKVAGRWVYLYR
                     AIDQYGQVIDVLVSEKRDLTATRRFFTQALDHGTQPSEVTTDRAPAYPRVLDELVPAA
                     CHITEQYANNLVEADHGRLKARLRPMRGLKQLRSTQVISAGYAFVQNLRRGHYDLGWD
                     VDPKHRRLTTAFTELALAI"
     gene            complement(1157..1680)
                     /locus_tag="VHH53_13070"
                     /pseudo
     CDS             complement(1157..1680)
                     /locus_tag="VHH53_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          299 a          610 c          541 g          324 t
ORIGIN      
        1 cagcggcgtg aaccgctgca cccaccgata gatagtcaca tgatcgacct cgataccccg
       61 ctcggcccgc gtgatcagtg ccggacacgc attcgtccaa aatctccgcc gcggtcacta
      121 cgaactcagc gtggaactcg acccacagca tcggcttcgg cggccttcgc cgaactcgtc
      181 ctcgccatct gaccgggccg ccaccccgat gagccgtacc cgcttcccgt caacgcaaca
      241 atgccgtccg acgtacccga ccgatccgca gactccgacc tctgatcctg ggccggggcc
      301 tccaccccag cagagtcctc ggcgacctca cccgaggacc ggtccgacgg gcactgttgc
      361 atcggggatc atcgcgagcg ggcaggctgg gttgatgcgt cgttgccctg cccaagtgtc
      421 cgcaccgaga tcgggcttcg ccgggtttcg gttcccgccg gaggtgatca cctgggcggt
      481 gcggtggtac ctgcgctttg gactttccta ccgtgacgtg gaggagctgc tggccgagcg
      541 gggtatcgag gtcgatcatg tgactatcta tcggtgggtg cagcggttca cgccgctgct
      601 gatcgacgcg gcgcgaccct gccggcacgt tccaggtgat cggtggtttg tggacgagac
      661 gtatgtgaag gttgccgggc gctgggtcta tctgtatcgg gcgatcgatc agtacggcca
      721 ggtcatcgac gtactggtgt ccgagaagcg ggacttgacg gccacccgcc ggttcttcac
      781 ccaggctctc gatcacggca cccagccgag cgaggtgacg acggacagag caccggccta
      841 tcctcgtgtg ctcgacgagc tggtgcccgc tgcctgccat atcaccgagc agtacgcgaa
      901 taaccttgtc gaagcagatc atgggcggct caaggctcgg ctgcggccga tgcgcggcct
      961 caaacagctt cgctctaccc aagtgatcag cgccgggtac gccttcgtcc agaacctacg
     1021 ccgcggccac tacgatctcg gctgggatgt cgacccgaaa catcggcggc tcacgacggc
     1081 cttcaccgaa ctcgccctcg ccatctgatt gtgcgcgatg gcagctcatc tcgcccattt
     1141 tcggtcaacg caacaggacc ttctggacga ttgcgtgccc ggcgctgatc acccgtgctg
     1201 agcgcagccg tttcaggccg cgcatcggcc gtaaccgggc cttcaggcgc ccgtgatcgg
     1261 cttcgacgac gttattcgca tactgctcgg tgacgtggca cgcggcaggt gttagctcct
     1321 cgatcactcg cgggtaggcc ggtgcccgat cggtggtcac ctcagccggc cgtgggccgt
     1381 gctccagggc accggtgagg aaccggtggg cggccgcctg atcccgcttc gggaagacca
     1441 gcacgtcgat gacctggccg tactggtcga tcgcccgata cagatacacc caccggccgg
     1501 cgaccttcac atacgtctca tccacgaacc aacaatctcc caggacgtgc cggcagggcc
     1561 gtgccgcgtc catcagcagc ggagtgaacc gctgtaccca ccgatagatg tcacgtgatc
     1621 caccgtgatg ccccgttcgg ccagcagctc ctccacgtct cggtacgaca gtgcgtagcg
     1681 cgaggtaggc cgtcaggcga ggtgccactt gcgtggtcct actcccgacg gccccctccc
     1741 gaaccggata tgatcgtttc cggtcgtcca gctc
//
LOCUS       DATADN010001014         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_433146, whole genome shotgun sequence.
ACCESSION   DATADN010001014 DATADN010000000
VERSION     DATADN010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433146"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VHH53_13075"
     CDS             complement(<1..989)
                     /locus_tag="VHH53_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291093.1"
                     /translation="MTAPMDIARQVADAVLYEGYLLYPYRASSRKNQVRWQWGVLMPS
                     GYAAAGHGEHATSHSECLLEPGTDPVLHIRLRFLQLQHRSGGDGPVPEFDEAVEHEID
                     SVLPVSELLDTEQVVAVTVPGGTETTDGVTRQRWPLNGEVRLSARRLEGPYGVLQLTI
                     EVVNTAQWVDPEAGRHLALRHSLIAAHTVLAVTDGEFISLIDPPHWAKPAVEVCRNER
                     TWPVMIGEAGRRDVILVSPIILYDYPTIAPESPGDLFDGTEIDEILTLRTMTLTDEEK
                     AEARATDERVRKLMDRVDSMPPEMLDKLHGAIRYLGETPGTRKEPDSIETITTP"
     gene            complement(986..1639)
                     /locus_tag="VHH53_13080"
     CDS             complement(986..1639)
                     /locus_tag="VHH53_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEX2291094.1"
                     /translation="MAELSFTCIDVQYERYAMTPTLLFRLRIDAAPGQRVRAIALRCQ
                     IRIEPARRGYGEQEGQRLNELFGERGRWGETLKPFQFANTSTVVASFTDSIEVDVVVP
                     CSYDMEVTAGKYFHALEDGEIPFILLFSGTIFGDAEKGMWVEQIPWHAETQYRMPVAV
                     WREMMDIHFPHSGWLRLRRDTIDALTEFRTAHALPTWDDAVMALLDAARERAVEGGS"
BASE COUNT          298 a          581 c          537 g          316 t
ORIGIN      
        1 ggggtggtaa tcgtctcgat cgagtccggc tcctttcggg tgcccggcgt ctcgccgagg
       61 tagcggatgg cgccgtgcag cttgtccagc atctccggag gcatcgagtc gacccgatcc
      121 atcagcttgc ggacccgctc gtcagtggcc cgtgcctcgg ccttctcctc atcggtgagc
      181 gtcatcgtgc gtagggtaag aatctcgtcg atctcggtgc catcaaagag atcgcctgga
      241 ctttccggcg caatcgtcgg gtagtcgtag aggatgatcg gcgagaccag gatcacgtcg
      301 cggcgcccgg cttcgccaat catcaccggc caagtgcgtt cgttgcggca gacctcgacg
      361 gcgggtttgg cccagtgcgg tggatcgatc aaggagatga attcgccgtc ggtgaccgcg
      421 agcaccgtgt gtgctgcgat caacgaatgc cgcaacgcca ggtgtcgacc ggcctctgga
      481 tcaacccact gcgcggtgtt gaccacctcg atggtgagct gcagcacgcc atacggaccc
      541 tccagccgcc gcgcggacaa ccggacttcg ccattgaggg gccagcgctg ccgggtcacg
      601 ccatccgtgg tctcggtgcc gccggggacg gtcaccgcaa ccacctgctc ggtgtccagg
      661 agctccgaaa ccggcagcac cgagtcgatc tcgtgttcca ctgcctcgtc gaactcgggc
      721 accggcccat ctccgccgct gcgatgctgc aactgcagga accgcagccg gatatgcaat
      781 accgggtcag tgccaggctc cagcaggcac tcgctgtgcg aggtggcgtg ctcaccgtgc
      841 ccggcggcgg catagcccga gggcatcagc actccccact gccagcggac ctggttcttg
      901 cgtgatgacg cacggtaggg atacagcagg taaccctcgt agaggacggc gtcggcaact
      961 tgccgggcga tatccatcgg ggccgtcatg atcccccttc cactgcgcgt tcccgggccg
     1021 cgtccagcaa agccatcacg gcgtcgtccc acgtgggcaa ggcatgggcg gttcgaaact
     1081 ccgtgagcgc gtcgatcgtg tcccggcgca gccgcagcca cccactgtgg gggaagtgaa
     1141 tatccatcat ctcgcgccac accgctaccg gcatccggta ctgggtctcg gcgtgccagg
     1201 ggatctgctc aacccacatc cccttctcgg cgtcaccaaa tatggttccg ctgaacagga
     1261 gaataaacgg gatttccccg tcctccagcg cgtggaaata tttacctgca gtgacctcca
     1321 tgtcgtagct gcacggcacg accacatcaa cctcgatgct gtcggtgaag ctggcgacga
     1381 cggtcgaggt gttggcgaac tggaatggtt tgagcgtttc accccagcgt ccgcgttcgc
     1441 cgaacagctc gttcagccgc tggccctcct gctcgccgta cccacggcga gcaggttcga
     1501 tcctgatctg gcaacgcagc gcgatggctc ggactcgctg cccgggcgcc gcgtcgatcc
     1561 gcagccggaa cagcagcgtc ggtgtcatgg catagcgctc gtactgcaca tcaatgcagg
     1621 tgaaggacag ttcagccatt ggtggccgtc acccgctcgc tgcgctcccg cagatcggcg
     1681 aagaactggt gaatctccgc ccaggccttc tcgccgccgt cgaagccctg cc
//
LOCUS       DATADN010001015         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_725818, whole genome shotgun sequence.
ACCESSION   DATADN010001015 DATADN010000000
VERSION     DATADN010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725818"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VHH53_13085"
     CDS             <1..582
                     /locus_tag="VHH53_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HEX2291095.1"
                     /translation="AEEWVQRLRATLPELPWQRQARIQAQWGLSDLELRDLVNAGALD
                     LIAATVDAGAPAAEARSWWVSYLSQQANSRGVELAELPITPEQVARVIALIGEGSLTN
                     NLARQVVDGVLAGEGQPDDVVAARGLAVVSDEAALTAAVEQALAAQPDIAEKIRGGKV
                     AAAGAIVGAVMKATGGQANAQRVRELVLALVSA"
     gene            complement(748..>1940)
                     /locus_tag="VHH53_13090"
     CDS             complement(748..>1940)
                     /locus_tag="VHH53_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2291096.1"
                     /translation="PTVRSTVSGLPGARCRAAAVVLAAAALLAGCSVTPDFPERDAGP
                     FRLKPEIGPQAEPRPEIPRPPGAAGPAPNGRPSPGPPRGCVDPDPQVVATCLEPISAL
                     VVLPGSALAGLAAERTTGRILRVQRERPTQEVSTLAVDPSGDGGLTGLVLSPSFAEDE
                     LIYAYITTPIDNRVVRIAPGDVPKPVLVGIPRGPTGNAGALAIDSHGMLLVATGNAGN
                     PAAATDPRSLAGKVLRIDTSGRPDPRNAAGSKVLVSGLHAPGGLCIDPVTNELWITDQ
                     KPAHHLLHRVQPGNPLGLASWTWPANRGAAGCVVMLGRIQVAFRSGAPMFAVGIGPDG
                     TFVGQPLEIPLQRYGRVSAAALSPDGQMMWLGTVNKMGGTPISSDERVFVLPDPVGPG
                     GGHD"
BASE COUNT          298 a          669 c          670 g          303 t
ORIGIN      
        1 gctgaagagt gggtgcagcg gctccgggcg acgcttcccg agctgccctg gcaacgccaa
       61 gctcggatac aggcccagtg gggcctgtct gacctcgagt tgcgcgatct ggtcaacgcc
      121 ggcgcgctgg acctcatcgc agccaccgtc gatgcgggag cgcccgcagc cgaggctcgg
      181 tcgtggtggg tgtcgtacct gtcccagcag gccaactcgc gtggtgtgga gctcgccgag
      241 ctgccgatca ccccggagca ggtggcccgg gtgatcgcgc tgatcggcga gggctcgttg
      301 accaacaacc tggcccgcca ggtcgtcgat ggtgtgcttg ctggcgaggg tcagcctgac
      361 gacgtggtcg ccgcccgggg cctggccgtg gtgtccgatg aggccgcgct gaccgctgcc
      421 gtcgagcagg cgctggctgc ccagcccgac atcgccgaga agatccgggg tggcaaggtg
      481 gccgccgccg gggccatcgt gggcgcggtg atgaaggcaa ccggtggcca ggccaacgcc
      541 caacgcgtcc gcgaactggt ccttgcgctc gtgagtgcgt aacagtgttc cagcactgaa
      601 tgccacctct tgctggagca gcacggggcc atcgatacgc accagcttgc ctccacgacc
      661 gaccaggttg catgccgcac ttctctacgc cggcccttcg gccatcctga gccaccgcac
      721 cgctgcggag gaatggggca tgctgcgtta gtcgtgccct ccaccgggac ctactgggtc
      781 gggtagcacg aacacccgct cgtcgctgct gatcggcgtg ccacccatct tgttgaccgt
      841 gcccagccac atcatttgcc catcagggct cagcgcggcg gcggacacgc ggccatagcg
      901 ttgcagcggg atctccaagg gctggccgac gaacgtgccg tccggtccga tccctaccgc
      961 gaacatcggc gccccactgc ggaacgcgac ttggatacga cccagcatga ccacgcaacc
     1021 cgccgcaccc ctgttggcgg gccaggtcca ggaggccaga cccagcggat tacccggctg
     1081 cactcgatgc agcagatggt gtgcgggttt ctggtcagtg atccacaact cgttggtgac
     1141 cgggtcgatg cacagcccac ccggtgcgtg caatccgctg accagcacct tggacccggc
     1201 cgcgttgcgc gggtccggcc ggccggaggt gtcgatccgc aacaccttgc cggccagcga
     1261 ccgtgggtct gtggctgcgg cggggttacc ggcgttccca gtggccacca gaagcatccc
     1321 gtgcgagtcg atggcgagcg cgcctgcgtt gcccgtcgga ccgcgcggga tgccgaccag
     1381 caccggcttg ggcacgtcgc cgggtgcgat ccggacaacc cgattatcaa tgggggtggt
     1441 gatgtaggcg tagatcagtt cgtcctcggc gaagctcggg gacaacacaa gcccggtgag
     1501 tccgccgtca cccgaaggat ccactgctag agtgcttacc tcctgggtcg gccgctctcg
     1561 ctgtacccgc aggatccgac ccgtggtgcg ctccgcggcc agacccgcta gtgccgagcc
     1621 cggcaacacg accagggcgg agatcggttc cagacacgtc gcgaccacct gagggtccgg
     1681 gtcgacacag ccccgtggtg ggcctggtga tggccgcccg ttcggggccg gaccggctgc
     1741 cccgggcggc cttggaatct cgggcctcgg ctcagcctgc ggcccaatct cgggtttgag
     1801 gcggaatggt ccggcgtcgc gctccgggaa gtcgggcgtc accgagcatc ctgctagcaa
     1861 tgccgccgcg gcgagcacca cagcggccgc gcggcagcgg gcaccgggca aacccgacac
     1921 ggtggatcgc acggtcggcc
//
LOCUS       DATADN010001016         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1035881, whole genome shotgun sequence.
ACCESSION   DATADN010001016 DATADN010000000
VERSION     DATADN010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035881"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VHH53_13095"
     CDS             <1..828
                     /locus_tag="VHH53_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2291097.1"
                     /translation="LTTVWACGPVVAAAVRAGWIAGTLFGAAVSIADIWVRGFFSTDL
                     ARDTILLVGTGFVLGLVATAARRATEQLRSASRAEAATAERERLARSVHDSVVQVLAR
                     VRAQGGHLDGEAGELARLAGEQEIALRCLFHAAPPGTTGEQDLAAALQQLASPRVEVS
                     VPATVVPLPACDVYELAAVVREALANTARHGGPEAKSWVLVEDLGDEVVVTVRDDGPG
                     VEEAQLADAEAGGRMGVSRSIRGRVADLGGTLTLETAPGQGTEWEVRLDRVRLGVLQ"
     gene            825..1472
                     /locus_tag="VHH53_13100"
     CDS             825..1472
                     /locus_tag="VHH53_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2291098.1"
                     /translation="MTARVMVVDDHPMWRAGVARDLGERGFDVVATAADGAAALRIVP
                     AASPDVVLMDLHFATGGPSGAETTRRIIDNWPDIRVLVLSASGEQADVLAAVQAGASG
                     YLVKSASVEELVDAVRRTAEGEVVFSAGLAGLVLGEYRRMALDPRTDLPRLTEREIEV
                     LRQVAKGRTARQIATQLGISHRTVENHVRSTLGKLQLHNRVELARYAIEHGLADQ"
BASE COUNT          258 a          575 c          663 g          317 t
ORIGIN      
        1 ctcaccacgg tgtgggcctg tggaccggtg gtcgccgccg cggtgcgtgc cggctggatc
       61 gcggggacgc tgttcggtgc tgccgtgtcg atcgccgata tctgggtgcg cggattcttc
      121 agcaccgacc tcgcccgcga cacaatcctg ctggtcggta ccggttttgt gctcgggctg
      181 gtcgctacag cggcgcgccg ggcgaccgag cagctacgca gcgcgtcccg cgccgaggcg
      241 gccaccgccg aacgggagcg gttggcccgc tccgtccacg acagcgtcgt gcaggtgctc
      301 gcccgagtgc gggcccaggg cggccacctc gacggggaag ccggtgaact ggctcggctg
      361 gccggggagc aggagatcgc gctgcgttgc ctgttccacg ccgccccacc gggcaccacc
      421 ggtgagcagg acctggcggc cgcgctacaa caattggcca gcccccgggt cgaggtctcg
      481 gtgcccgcga ccgttgtgcc gttgccggcc tgcgacgtct atgagctcgc cgctgtcgtt
      541 cgggaggccc tggccaacac cgcccggcac ggcggtccgg aggcgaagtc gtgggtgctg
      601 gtcgaggatc tcggcgacga agtggtcgtc accgtccgcg acgacggtcc cggtgtcgag
      661 gaagctcagc tcgctgatgc cgaggcgggc ggccggatgg gagtgtcgcg ctcgatccgc
      721 ggtcgggtgg ccgatctggg cggcaccctg accctggaaa ccgctcccgg tcagggcact
      781 gaatgggagg tacggctaga tcgcgtccgg ctgggcgtgc tgcagtgacg gctcgcgtca
      841 tggtggttga cgaccatccg atgtggcggg ccggcgtggc ccgagacctc ggtgagcgag
      901 gctttgacgt ggttgctacc gcggcggacg gtgcggctgc actacgtatt gtgccggccg
      961 ccagcccgga cgtcgtcttg atggatctgc acttcgccac tggtggaccg tcgggcgctg
     1021 agaccacccg gcgcatcatc gacaactggc ccgacatccg ggtgctggtg ctgtccgcct
     1081 ctggcgagca ggccgatgtc ctggccgcgg tgcaggcagg tgcgtccggg tacctggtga
     1141 agtcggcgtc ggtggaggag ctggtcgatg ccgtgcgccg gaccgctgag ggagaggtgg
     1201 tgttcagcgc cggccttgcc ggcctggtgc tcggggagta ccgccggatg gcgctggatc
     1261 ctcgaacgga tttgccgcgg ctcaccgagc gtgagatcga ggtgctgcgg caggtggcca
     1321 aggggcgcac cgcccgccag atcgccaccc aactgggaat cagtcaccgc accgtggaga
     1381 accacgtacg gtccaccttg ggcaaactgc agttgcacaa tcgggtcgag ctggcccgct
     1441 acgcgattga gcacggcctc gccgaccagt aggtgcctct tcaagtgccg ccaacccccg
     1501 cgttgatcat cgtaggccga tatcaacctg gcgctgcctg ctcagctagc tggcatcgcg
     1561 gagcgcggac gttggtaggt tacgagctgc cccaaccggg gttggcggta ttcgaagagt
     1621 gactgaccat ggcccggctg ggctggccca cggtgtgatg tccgtttcga ctcagtgggg
     1681 accgtaccgc tcggcggttt taacgcgaac gcagtagcta gaatcgcttt acgcgatggc
     1741 tcataagctg attagctgct tgccgaacgg ggtatccaat agtcgcgtgc gttcgggtgc
     1801 agatcgtcct gac
//
LOCUS       DATADN010001017         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_865921, whole genome shotgun sequence.
ACCESSION   DATADN010001017 DATADN010000000
VERSION     DATADN010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865921"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VHH53_13105"
     CDS             <1..474
                     /locus_tag="VHH53_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2291099.1"
                     /translation="GGGVWALRYHNVYGSRMPRDTPYAGVASIFRSALAGGAPPQVME
                     DGQQRRDFVHVSDVAQANALALAAEPPLQRCEPVNVCSGHPHTVGELAAELARAMNGP
                     TPQIVGGARPGDVRHVTADPARAAELLGYRAQVGFAEGVTRFATDPLREPITTSP"
     gene            complement(689..2047)
                     /locus_tag="VHH53_13110"
     CDS             complement(689..2047)
                     /locus_tag="VHH53_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291100.1"
                     /translation="MTRTEPRKPLTRSRDFRADLAAVGSAALLVTAAAVVGVELLQLG
                     VPLHVDAPPLGAQWLPHVGRGTVPAVLIAILVILRGPELAVRLPWRLLLGISTVAALA
                     WTMSLALIDGWQRGVAGRLTTDTEYLHDVPRVSDVGAMLRIFAERILGLQPESWTTHV
                     AGHPPGALLVFVGLDRIGLPGGVPAALLCVLVGSTAGIAVAVTLRALGAEAAARAALP
                     FSVLFPGAIWIGVSADGLFAGVLAWGVALLALGASRPRLDVLTVVASAAGGLVLGASL
                     YLSYGLVLAGLLPLAVAITTRRIVPLLVAGAGVVTVVAAFAAAGFWWFDGYQQVVIRY
                     YQPGEYGLERPYSYWVWANVACLAVVLGPAGVAGLRRVFTPGRGQPRALLALCVAAAL
                     AVLAADLSGLSKAEVERIWLPFAVWLVAAAALLPARHVRWWLSAQTVIALAINHLLLT
                     TW"
     gene            complement(2109..>2315)
                     /locus_tag="VHH53_13115"
     CDS             complement(2109..>2315)
                     /locus_tag="VHH53_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc permease"
                     /protein_id="HEX2291101.1"
                     /translation="GTAVGHELTNDAVSMVFLTLAAGSILYVIIHLVAIALAGGHRRL
                     LYWGVWAGLVAGFVIDMLLTAAGA"
BASE COUNT          412 a          832 c          787 g          284 t
ORIGIN      
        1 ggcggggggg tgtgggcact gcgctaccac aacgtctacg gctcccggat gccccgggac
       61 accccgtatg ccggagtggc gtcgatcttc cgatcggcgc tggcgggtgg tgcgccaccg
      121 caggtgatgg aggacgggca gcagcgtcgg gacttcgtgc acgtgtccga cgtggcccag
      181 gccaacgcgc tggccctggc tgccgagccg ccgttgcagc ggtgcgagcc ggtcaacgtc
      241 tgctccgggc acccgcatac ggtcggcgag ctggccgccg agttggcccg cgcgatgaat
      301 ggaccgactc cccagatcgt cggtggtgcc cggcccggcg atgtgcggca tgtgacggcc
      361 gaccccgccc gggccgctga gctgctcggt taccgggcgc aggtgggctt cgccgagggc
      421 gtcacgcggt tcgccaccga tccactgcgc gaaccgatca cgacgtcgcc ctaaaacggc
      481 gggaggtcgt cgctggggac ggattcggct ggctgcggtt tcggtgggag tccaggcggc
      541 ggcgctaccc aaatctccga ggtttcccag tcggcgtcga ccgggaccac cagtgggatg
      601 gaggctgcgc ccgggtagcg cgagattcgt gcgccgcccc gcgcgccgga agaggtgcac
      661 agcgtccagg gtcctcctca gaagccaatc accacgtcgt gagcagcagg tggttgatcg
      721 ccagcgcgat caccgtctga gcgcttaacc accagcggac atgccgcgcg gggagcagcg
      781 cggccgcagc gaccaaccag accgcgaatg gtagccagat ccgctccacc tcggccttgc
      841 tcagcccgga caggtccgcg gccaacaccg ccagtgccgc cgccacgcac agtgccagca
      901 gcgcgcgcgg ttggcctcgc cccggagtga acacccggcg caacccggcc accccggccg
      961 gtccgagcac caccgccagg cacgccacgt tggcccagac ccaatagctg tacgggcgct
     1021 ccaggccgta ctcacccggc tggtagtacc ggatgaccac ctgttgatac ccgtcgaacc
     1081 accagaagcc cgcggcggcg aaggccgcca ccacggtgac gacaccggcg ccggccacaa
     1141 ggagcgggac gatccggcgg gtggtgatcg cgacggccag cgggagcaag cccgccaaca
     1201 ccagcccgta ggacaggtac aagctggcgc cgagaaccag cccgccggcc gcggacgcga
     1261 caacggtcag cacgtcgagc ctggggcggg aggcaccgag ggcaagcagg gccactcccc
     1321 acgccagcac gccggcgaac aggccgtccg cggacacccc gatccagatg gcaccgggaa
     1381 acagaacgct gaacggcagc gcagcccggg cggcagcttc ggcgcccagt gcccgcagcg
     1441 tgaccgccac tgcgatcccg gccgtcgacc cgaccagcac gcacagcagt gccgccggca
     1501 ctcccccggg cagcccgatc cgatccaggc cgacgaagac cagcagtgcc ccggggggat
     1561 ggccggccac atgcgtggtc caggactcag gctgcaggcc cagtatccgc tcggcgaaga
     1621 tccggagcat cgcaccaacg tcgctgactc tagggacgtc gtgcaggtac tcggtgtcgg
     1681 tggtcaacct accagcgaca ccgcgttgcc acccgtcgat cagggccagg ctcatcgtcc
     1741 aggccagcgc cgcgacagtc gagataccga gcagcagtcg ccagggcagc cggacggcca
     1801 gctcgggtcc gcgcaggatg acgagaatcg cgatcagcac cgcgggaacc gttcccctgc
     1861 cgacgtgcgg cagccactgc gcgcccagtg gtggggcgtc tacgtgcagc ggcaccccta
     1921 gttgaagcag ctcaacgccg accactgccg ccgccgtgac gagcaacgcg gcgcttccca
     1981 cagccgccag gtccgcgcgg aagtcccggg atcttgtcaa cggcttgcgc ggctcagttc
     2041 tcgtcacgcc tgcagagtct tatccctctg gcgtacctga tgcgacgaac accggcagtg
     2101 gcttgaggct aggcaccggc agcggtgagc aacatgtcga tgacgaagcc ggcgaccaga
     2161 cccgcccaca cgccccaata aagcagacgc cgatgaccgc ccgcgagggc gatcgcgacg
     2221 agatggatga tcacgtagag gatcgagccg gcggccaagg tcaggaaaac catgcttacc
     2281 gcgtcgttgg tcaactcgtg cccgaccgcg gttcc
//
LOCUS       DATADN010001018         4111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_433483, whole genome shotgun sequence.
ACCESSION   DATADN010001018 DATADN010000000
VERSION     DATADN010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433483"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VHH53_13120"
     CDS             complement(<1..226)
                     /locus_tag="VHH53_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2291102.1"
                     /translation="MRRRILDLLLERPRLVGELTERLGLSQPSTSKHLRTLREAGLVQ
                     VRPDAQRRWYELRPEPLAEVDAWLAPYRRLW"
     gene            complement(236..1525)
                     /locus_tag="VHH53_13125"
     CDS             complement(236..1525)
                     /locus_tag="VHH53_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX2291103.1"
                     /translation="MSARQIVVIAVLGMLALAVPPSASAAPPAPSEPVSAYRVQGLND
                     SAARTAVLHQGVDVLGGGQDYLEIRATSRQADRLRAGGLRLLPLPALPAVPGVVAPGQ
                     FPIGYRGYHTYDKLTAELGAIAAARPDQVAVSSYGRSTEGRPLPLVKISDAVRTDQNK
                     PEVLFSCAQHAREHLTVEMCLHIVRRLAQGYGTDPAITELVRSREIWVVPTANPDGTE
                     YDISSGLFALWRKNRQPTQENTEIGTDPNRNWGTEWACCGGSSTDPGSDTYHGTAPFS
                     APETAQLRDWVNSRVIGGVQQITAHIDFHSYSELVLWPYGFTTDDTGPGLNASDAATF
                     RRLGEAMAATNGYTAQQSSDLYITDGTVGDWMWAQHGIWSYTFEMYPTSQVQGGFYPP
                     ETEIKREVERNDAAVDLLLDYADCVRRITGDLCDPEQ"
     gene            1615..2940
                     /gene="hisD"
                     /locus_tag="VHH53_13130"
     CDS             1615..2940
                     /gene="hisD"
                     /locus_tag="VHH53_13130"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX2291104.1"
                     /translation="MLSRTDLRGHLPAPATLRGLLPRAEVDVDAVADQVAPLIEAVRD
                     RGVAAVLEFTERFDKVRPSRIRVPADALRAALAQLDPTVRAALEESISRVHQAHVDQQ
                     RTETVTHVVPGGTVTQRWVPVQRVGLYAPGGLAVLASTVVMTVVPAQVAGVRSLVMCS
                     PPRAAHGGLPHPTLLAAAALLGIDEVWAAGGAQAVALLAYGGTDTDGAPLDPVDMVAG
                     PGNIYVTAAKRALRGTVGIDAEAGPTEIAILADDTADPVHVAADLVSQAEHDTLAASV
                     LVTDSVALADAVDVELHRQVPATKHTDRIRTALTGPQSGCILVSDVDAGLSVVDAYAA
                     EHLEIQTAGAAAVARRVRNAGAIFVGAYAPVSLGDYCAGSNHVLPTGGCARHSSGLSV
                     QSFLRGVQLIEYDEDALVKVGDHVVALAEAEDLPAHGQAITARFRGTAS"
     gene            2946..4097
                     /locus_tag="VHH53_13135"
     CDS             2946..4097
                     /locus_tag="VHH53_13135"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX2291105.1"
                     /translation="MAGGIGCDVTLADLPLRDDLRGCSPYGAPQLDVAVRLNTNENPY
                     PPPPALVADIAAAVQQEVCELHRYPDRDAVALRTDLAGYLAGRTGVPLTVANMWAANG
                     SNEIIQQLLQAFGGPDRSALGFTPSYSMHPIISAGTRTRWIPAPRRADFSLDPEGAVA
                     AIEEHRPDVLFVTSPNNPTGQSIPLVDLAAVLAAAPGIVVVDEAYGEFSNARSATGLL
                     GQFPDRLVICRTMSKAFAFAGGRLGYLAAAPAVVDALLLVRLPYHLSALTQVAARAAL
                     RHGAATLESVAVLVRERERVIAALRDLGAEVVPSDANFVLFGRFGDAATAWRRYLEHH
                     VLVRDVGIEGHLRVTVGTPAENDAFLAASAGVLETCSQGRNIDAAKEEP"
BASE COUNT          590 a         1409 c         1437 g          675 t
ORIGIN      
        1 cccacagccg ccggtagggc gcaagccagg catcgacctc agctaacggc tcgggccgca
       61 gctcatacca gcggcgctgg gcgtccgggc ggacctgcac cagaccggcc tcacgcagcg
      121 tccgcaagtg tttggaggtg ctcggctggc tcagacccag cctctcggtc agctcaccga
      181 ccagccgggg acgctcgagc aggagatcga gaatgcgccg cctgatcggc tcggcctatt
      241 gctcaggatc gcagagatcc ccggtgatcc gccggacgca gtccgcgtaa tccagcagca
      301 ggtcaaccgc ggcgtcgttg cgctccacct cgcgcttgat ctcggtctcc ggcggataga
      361 agccgccttg cacttggctg gtcgggtaca tctcgaaggt gtacgaccag ataccgtgct
      421 gcgcccacat ccagtcgccc accgtgccgt cggtgatgta gaggtcgctg gactgctgtg
      481 cggtgtagcc attggtggcg gccatcgcct cgcccagccg ccggaaggtt gccgcgtcgg
      541 aggcgttcaa gcccgggccg gtgtcgtcgg tggtgaaacc gtagggccag agcaccagct
      601 cggagtagct gtggaagtcg atgtgcgcag tgatctgctg cacgccgccg atcaccctgc
      661 tgttcaccca gtcccgcagc tgcgccgtct ccggagcgga gaacggcgcg gtgccgtggt
      721 aggtgtcgct accggggtct gtgctcgaac cgccgcagca ggcccactcg gtgccccagt
      781 tccggttggg atcggtgccg atctcggtgt tctcctgcgt cggttggcgg ttcttgcgcc
      841 acagtgcgaa aaggccgctg gagatgtcgt attcggtgcc gtccgggttg gccgtcggca
      901 ccacccagat ctcccgcgac ctcaccagct cggtgatggc cgggtcggtg ccgtagccct
      961 gtgccagccg ccgcacgatg tgcaggcaca tctcgacggt gaggtgctcg cgggcgtgct
     1021 gcgcgcagct gaaaagcacc tcgggcttgt tctggtcggt gcggaccgcg tcgctgatct
     1081 tcaccagcgg tagcggacgt ccctcggtgg agcggccgta gctggatacc gcaacctgat
     1141 cggggcgggc tgcggcgatc gcgcccagct cggcggtgag cttgtcgtag gtgtggtagc
     1201 cgcggtatcc gattgggaac tgaccgggcg ccaccacgcc gggcaccgcc ggtagtgccg
     1261 gaagcgggag cagccgcagc ccgccggccc gcaaccgatc ggcttgcctg gatgtggccc
     1321 ggatctccag gtagtcctgc ccgccgccga gcacgtccac gccctggtgc agcactgcgg
     1381 tgcgcgcggc tgagtcgtta agtccttgca cgcggtatgc cgacaccggt tcggacggcg
     1441 ccggtggtgc cgcactggcc gagggtggga cggccagcgc aagcatcccc agcacagcga
     1501 tcacgacgat ctgacgcgca gacatttcgg gaactctaat ggcggaggcg tcgctcgtta
     1561 caggcagatc gacgcgcggc ggcaccgggc cggcggcgca ttacgctgaa ctgcatgctc
     1621 agccgtaccg acctgcgcgg tcaccttccc gctcccgcga cgctgcgcgg cctgctgccg
     1681 cgggccgaag tggacgtcga tgcggtggcc gatcaggtcg cgccgttgat cgaggcggtc
     1741 cgtgaccggg gcgtggcagc ggtgctggag ttcaccgaac gctttgacaa ggtgcggccg
     1801 agccgtatcc gggtacccgc cgacgcgcta cgcgccgcgc tggctcagct tgacccaacg
     1861 gtgcgcgccg ctctggaaga atcgatttcc cgggtccacc aggcacacgt cgaccagcag
     1921 cgcaccgaga cggttactca cgtggtgccc ggtggcaccg tcacgcagcg gtgggtgccg
     1981 gtgcaacggg tcgggctcta cgcaccagga gggctggcgg tcctcgcgtc aaccgtggtg
     2041 atgaccgtgg tacctgccca ggtcgccggg gtcaggtcac tggtgatgtg ttctccaccg
     2101 cgggctgccc acggtggcct gccgcacccc accttgctcg ccgcggccgc cttgctcggc
     2161 attgatgagg tgtgggccgc cggaggagcg caggccgtcg cgctgctcgc ctacggcggc
     2221 acagataccg acggcgcccc cttggacccg gtggacatgg tcgccggccc cggcaatatc
     2281 tacgtcaccg cagccaagcg cgccctgcgc ggcacggtgg gcatagacgc tgaggccggc
     2341 cccaccgaga tcgccatcct ggccgacgac actgctgacc ccgtccacgt cgccgctgac
     2401 ctggtctccc aggccgagca tgacaccctg gccgccagcg tgctggtcac cgactcggtg
     2461 gcgctggccg acgcggtgga cgtcgagttg caccggcagg ttccggccac caagcacacc
     2521 gatcggatcc gcactgcact gaccggaccg cagtcgggct gcatcctggt gtccgatgtg
     2581 gacgccggcc tgtcggtggt ggacgcctat gcggccgagc acctggagat ccagaccgcc
     2641 ggcgcggctg cggtggcccg ccgcgtgcgc aacgccggag cgatcttcgt cggtgcctac
     2701 gcacctgtct cgctgggtga ctactgcgcc ggctccaatc acgtgctgcc caccggaggc
     2761 tgcgcccggc actcttcggg gctgtcggtg cagagcttcc tgcgcggtgt ccagctcatc
     2821 gagtatgacg aggacgcgct ggtgaaggtc ggtgaccacg tcgtcgcgct cgcggaagcc
     2881 gaagacctcc ccgcgcacgg tcaggcgatc accgcccggt tccgcgggac cgcgtcgtga
     2941 ccgtggtggc cggcgggatc ggctgcgacg tgacactcgc cgacctgccg ctacgcgatg
     3001 acttgcgggg atgcagtccc tacggtgcac cgcaactcga cgtggcggtg cggttgaaca
     3061 ccaatgagaa cccctacccg ccgccgcccg ccctggtcgc cgacattgcc gccgcagtgc
     3121 agcaggaggt ctgtgagctg caccgttacc ccgaccggga cgccgtcgcg ctgcgcaccg
     3181 acctggccgg ttacctcgcc gggcgcaccg gtgtgccctt gaccgtggcg aacatgtggg
     3241 ctgccaacgg gtccaatgag atcattcaac agctgctgca ggccttcggc gggcctgacc
     3301 gcagcgcgct gggcttcacg ccgtcctact cgatgcaccc gatcatctcc gcaggcacgc
     3361 gcacccgatg gatcccggca ccgcggcggg cggatttctc cttggatcct gagggtgcgg
     3421 tggccgcgat cgaggagcac cggcccgacg tgctgttcgt caccagcccc aacaacccca
     3481 ccgggcagtc gatcccgcta gtggacctgg cggccgtgtt ggccgccgcg ccgggcattg
     3541 tggtggtcga cgaggcctac ggcgagttca gcaacgcgcg cagtgccacc ggactgctcg
     3601 ggcagttccc ggaccggctg gtgatctgcc ggacgatgag caaggcgttc gcgttcgccg
     3661 gcgggcggct gggctacctc gctgccgcgc ccgcggtggt ggatgcgctg ctgctggtgc
     3721 ggctgcccta ccacctctcg gcgctgaccc aggtggccgc ccgggccgcg ctacgtcatg
     3781 gagctgccac cttggaatcc gtggcggtat tggtgcgaga gcgggagcgc gtgatcgcgg
     3841 cgctgcgcga tctgggcgcc gaggtggtcc cgtcggacgc caatttcgtg ctgttcgggc
     3901 ggttcggtga tgcggccacg gcctggcgcc gctacctgga acatcatgtt ctggtccgtg
     3961 atgtcggaat cgagggtcac ctgcgagtca ccgtgggcac ccccgcggag aacgatgcgt
     4021 tcctggccgc gagcgcaggg gtgcttgaga cctgctcgca gggtcgaaac atcgatgcag
     4081 ccaaggagga accgtgagcc gtacggccag g
//
LOCUS       DATADN010001019         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_265107, whole genome shotgun sequence.
ACCESSION   DATADN010001019 DATADN010000000
VERSION     DATADN010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265107"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            701..2074
                     /locus_tag="VHH53_13140"
     CDS             701..2074
                     /locus_tag="VHH53_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase
                     family protein"
                     /protein_id="HEX2291106.1"
                     /translation="MEYPFVHSELPGPRSAELLANQDRRESNARAYPRHLPIAIADAA
                     GSFVRDVDGNVFIDFLNGAGVLPLGHNPPELVHAVIGQLGVLTHGLDFPTPVKDAFTE
                     AQLSMLAPGMRSRMKVHFCGPTGANAVDAALKLCKTATGRGDVISFQGGFHGTTHAAM
                     ALTGLVANKAPVANGVPGVHFFPFSSCSSCPVGLTPQTCTTNCVHYLERALRDPNGGI
                     ALPAAVVMEMVQGEGGVVPAQPDFVRRVRELTRELDIPLVVDEVQTGCGRTGTWFAFE
                     HYGIEPDVIIASKALSGIGTPVAIILYDQRLDVWAPGAHTGTFRGNQLAFAAGVETIR
                     IFRRDDVLGNVRRRGDQIAGMLEGLRSNPWVHEVRGRGLMWGIELADPLDSRPAGELA
                     EQVQAAALRRGLILELGGRNDCVVRMLPPLNVTAEVIDTACSIFLEALQECCPTLWSP
                     GPLMSIN"
BASE COUNT          347 a          669 c          706 g          403 t
ORIGIN      
        1 gcggatcagc gcgagcacca ggtcgggctg ctcgtcggat cgccctggag atcgaggcgg
       61 tactcatcca gaccgctccc gcgtgacggg cagctggtgc cagaaagaaa tctggcattc
      121 accacgatcg gtcgccgagc ccgccgcgcg ctggctcgaa actcacggtg gccacggccc
      181 cttcgggttc ggtgtcgccg gcaccgccgc ttgctgttgc cagcgacgtc gcgactgccc
      241 tcgctgtcga gatcctgcac gccaaacgtg tcgcacttcg atccgttcgc ccacgagcac
      301 aagccgcacc cggtcagctc tccagcgctg cgaccatccg ggactgctcg ccggatcagc
      361 actggcgatc gactaccgca ccattgccgc gatcgccgag cagctcaagg cggtgacgcc
      421 cctcgtgcgc gctggtcccc gtacccgcat tcagcgggct cagcgttcca acagcagggc
      481 agattacccc gctcagcccg ctgaatacgg gaggtctgcc ggacgatctt cgcccgctcg
      541 gggatccttg ccgaaccaca caggagcgag gatcgaacta actcggtcat caaaaccgcc
      601 tgctgagtca agcaggtggc tcgctttcac atgcgtctat tcgggtgacg aagtgaaccg
      661 ttcagggggg attgtggctg cgcccatcga tcaaaatagg ttggagtatc cgttcgttca
      721 cagtgagttg ccggggccgc gttcggctga gctactggcc aaccaggatc gcagagagtc
      781 caacgccagg gcgtatccgc ggcatctacc tattgctata gcagatgctg ccggcagctt
      841 cgttcgtgat gttgacggca acgtgttcat cgacttcctc aacggtgctg gtgtgcttcc
      901 gttggggcat aaccctcctg agctggttca cgcggtgatc gggcagcttg gggtgttgac
      961 ccacggtctg gactttccga ctccggtcaa agacgccttc accgaggcgc agctgtccat
     1021 gctggcgccg ggcatgcgca gccggatgaa ggtgcatttc tgtggaccga cgggagccaa
     1081 cgcggtggac gctgcgctga agctgtgtaa gaccgcgacc ggccgcggtg acgtcatctc
     1141 ctttcagggt ggcttccatg gcaccaccca tgccgcgatg gcgctgaccg ggttggtggc
     1201 caacaaggca cctgttgcca acggcgtgcc cggcgttcat ttcttcccgt tctcctcgtg
     1261 cagcagctgc ccggtgggcc tgaccccgca gacctgcacc accaactgtg tgcattacct
     1321 ggagcgggcg ctgcgggatc ccaacggggg catagcgttg ccggccgcgg tggtgatgga
     1381 gatggtgcag ggtgaaggtg gggtcgtgcc cgctcagcca gacttcgtcc ggcgagtgcg
     1441 cgagctgacc cgagaactgg acattccgct ggtggtggat gaggtgcaga ccggctgcgg
     1501 ccgcactggc acctggtttg cttttgagca ctacggcatc gagcccgacg tcatcatcgc
     1561 gtcgaaggcg ctgagcggaa tcgggacccc ggtcgcgatc atcctgtacg accagcgcct
     1621 ggacgtctgg gctccgggcg cgcataccgg gaccttccgg ggtaatcagc ttgcctttgc
     1681 cgcgggggtt gagacgatcc ggatctttcg ccgcgacgat gtgctgggca acgtgcggcg
     1741 gcgcggtgac cagatcgctg ggatgttgga gggcctccgg agtaatccct gggtgcacga
     1801 ggtgcgcggc cggggcctga tgtggggtat cgagctcgct gatccgttgg acagccgacc
     1861 ggcaggtgag ctggcggagc aggttcaggc ggcggcattg cgccgcggac tgatcctgga
     1921 gctcggtggc cgcaacgact gcgtcgtgcg gatgttgcca ccgctgaatg tgaccgccga
     1981 ggtcatcgac acggcatgtt cgattttcct cgaagcgctc caggagtgtt gcccaaccct
     2041 gtggtcaccg gggccgctga tgtcgatcaa ctaattcgac cgcgtgtata tcgcgcgggc
     2101 gccgtcggta accccactgc cggcg
//
LOCUS       DATADN010001020         2583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_580824, whole genome shotgun sequence.
ACCESSION   DATADN010001020 DATADN010000000
VERSION     DATADN010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580824"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..466
                     /locus_tag="VHH53_13145"
     CDS             149..466
                     /locus_tag="VHH53_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX2291107.1"
                     /translation="MVLEIAEFTIRSGQEDQFALAYRRAAHLLRDTPGCLSMRMTRGV
                     ESPSRFVLLVEWDNITDHTEGFRGSTSFRSWREQLGPFFAEAPRVEHSRDIGSGDGLP
                     MGH"
     gene            522..>2583
                     /locus_tag="VHH53_13150"
     CDS             522..>2583
                     /locus_tag="VHH53_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2291108.1"
                     /translation="MSLTARLAWLRQRPVVLLLVAVLAAGAGVAVTVTVIPDPPQPAT
                     ETVMIDVPAGPDGTGQVRLDATVYRPAAIPAPAVLVAHGFGGSKNSVSAEATELARRG
                     FIVLAWTARGFGASGGQIGLNSPDYEVADARALVDWLADRPDVIRDGPGDPRVGVTGA
                     SYGGALALLLAGTDPRVDALVPQITWNDLAQALFPNAASRQPVYAGTPAAGAFAPDGV
                     FKREWAGLFFGSGADSSGVCGRFRSDVCAAYSQAATTGAPTPQIVALLRRSSPSAVTG
                     NITAPTLLIQGERDTLFGLDHADATARQLTSAGAPLKVVWYAGGHDGGELGGQLPEQI
                     SAWFDYHLRRVGENPGSGFEYQVQGAFGTRRGFAQRTVTVPAYPGLDGAPAQSQISVQ
                     RRELSLHGGPQQVVRPPGGSPAALSSLPGLSRALPEGAGNLDAVALDVPGQAATFSTD
                     PLNDPVEIVGAPRIRLQVAAAPTGSDAPPAEAVLFVKLYDLGPDQRRTLPGSAVAPVR
                     IGPLPADGTPVEVTVTLAGVVRPVQAGHRLELAVATTDRGYALPPQPARYTVTLPGDA
                     RLAVPVVPGEQAPVPLPVTQLVGLGGLAASVVALGIFAALRRRRAGDTAPELAEVPLV
                     VSGLAKRYPGGIIAVDDLSFRVEHGQVLGLLGPNGAGKPTVLRMLMGLIHPTAGELRT
                     FGHRI"
BASE COUNT          359 a          909 c          915 g          400 t
ORIGIN      
        1 agcactgcga gagccgtgat gacgatgccg ccgcgaagga atactcccct ccacgtaccg
       61 gacaagtctc ccgcatcgcc cctgccggca ccagccgggc ttgctaagac accacgtacg
      121 tggacgtagc gtgtgcgagt gtgtgcatat ggtgctggag atcgccgaat tcaccatccg
      181 atccggtcag gaggaccagt tcgcccttgc atacaggcgg gcggcgcacc tgctgcggga
      241 caccccgggc tgcctgtcga tgcgcatgac cagaggggtc gagtcaccgt cccggttcgt
      301 gctgctggtc gagtgggaca acatcaccga ccacaccgag ggtttccgcg ggtcaacgtc
      361 gtttcgcagc tggcgcgagc agctgggccc cttcttcgct gaggcacctc gggtggagca
      421 ctcgagggac atcggatccg gtgatggctt gccgatgggg cactgagaat ccgctgagac
      481 ggctgctccg ccgtcgaccc gcctcaccta ccgtcggcgg cgtgtccctc acggctcggc
      541 tggcctggct gcgccagcga cccgtcgtgc tgctactcgt cgccgtcctg gccgcgggtg
      601 ccggcgtggc ggtgaccgtc acggtgatcc cggatccgcc tcaaccggcc accgagaccg
      661 tcatgatcga cgtgccggct ggaccggacg gcaccggcca ggttcgcctg gatgccacgg
      721 tgtaccgacc agcagccatc cccgctccgg ccgtgctggt agcccacggt ttcggcggaa
      781 gcaagaactc ggtcagcgcc gaggccactg agctggcgcg ccgggggttc atcgtgctcg
      841 cgtggaccgc ccgtggattc ggcgcaagcg gcggccagat cgggctcaac tcccccgact
      901 acgaggtggc ggacgcaagg gcgctggtgg actggctggc cgaccggccc gacgtgattc
      961 gcgacggccc cggcgatccg cgcgttgggg tgaccggagc ctcctacggc ggcgcgctgg
     1021 ctctgctact tgccgggact gatccccggg tggacgccct cgtaccgcag atcacctgga
     1081 acgacttggc gcaggcgctg ttcccgaacg cggcgagccg ccaacccgtc tacgctggca
     1141 ccccggcagc aggcgccttc gccccggacg gagtgttcaa gcgagagtgg gctgggctgt
     1201 tcttcggctc tggcgccgac agttcaggag tatgcgggcg cttccgctcg gacgtgtgtg
     1261 ccgcctacag ccaggccgcc accaccggcg cgcccacccc gcagatcgtg gcgctgctgc
     1321 gccgctcgtc gccttccgcg gtgaccggca acatcaccgc gccgacgctg ctcatccagg
     1381 gcgagcggga caccctgttc gggctcgacc atgccgacgc gaccgcccgc cagctcactt
     1441 cggcaggtgc cccgctgaag gtcgtctggt acgcgggcgg gcatgacggc ggcgaactcg
     1501 gcgggcagct accggagcag atcagcgcct ggttcgacta ccacctgcgc cgggtcggcg
     1561 agaatcctgg tagcggcttc gaatatcagg tgcagggagc tttcggcacc cggcgaggtt
     1621 tcgcccagcg aaccgtcacc gtcccggcct atcccgggct cgacggtgcc ccggcacaaa
     1681 gccagatctc ggtgcaacgc cgggaactgt ccctgcacgg cgggccgcag caggtggtcc
     1741 gcccacccgg cggcagcccc gccgcgctca gcagcttgcc gggcctgtcc cgcgcgctgc
     1801 ctgaaggcgc cgggaacctc gatgcggtgg cactggatgt gcccggccag gccgcgacgt
     1861 tcagtactga tccgctcaac gacccggtgg agatcgtcgg ggcgccgcgg atccggttgc
     1921 aggtcgccgc tgcacccacc ggctccgacg cgccacccgc cgaggcggtg ctgttcgtca
     1981 agctctacga cctcggaccg gaccagcggc gcacgctgcc gggcagtgcg gtggcgccgg
     2041 tgcggatcgg gccgttgccc gccgacggca caccagtcga ggtgacggtg accctggcgg
     2101 gtgtggtacg accggtgcag gccgggcacc ggttggaact ggccgtggca acgaccgacc
     2161 gcgggtacgc gctgcccccg caacccgcca ggtacacggt gacgctgccc ggggacgccc
     2221 gcctggcggt gccggtggtg cccggcgagc aggcaccggt gccgcttccc gtcactcagc
     2281 tggttgggtt gggcgggttg gccgcgtcgg tggttgcgct cgggattttc gccgcgctgc
     2341 gccgccgccg cgccggcgac actgcaccgg agctggccga ggtgccgctg gttgtgtcgg
     2401 ggctggccaa gcgttacccg ggcggcatca tagccgtgga tgacctgtcg tttcgcgtcg
     2461 agcacggcca ggtgctgggg ctgctcggac ccaacggtgc cggcaagccc accgtgttgc
     2521 ggatgctgat ggggctgatc catccgaccg ccggggagct gcggacattc gggcaccgga
     2581 ttg
//
LOCUS       DATADN010001021         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_581503, whole genome shotgun sequence.
ACCESSION   DATADN010001021 DATADN010000000
VERSION     DATADN010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581503"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VHH53_13155"
     CDS             <1..1250
                     /locus_tag="VHH53_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX2291109.1"
                     /translation="DAQIDDLLNGIIDDLSNPGEGIEATFRWPYVPHAPMEENAAVAN
                     VTSDKAEVWGGAQIPPTLQRQLAETLGMPIEKVTYHVIRAGGAFGRHLFHDQDVHAAQ
                     VSQRLGKPVKLQWLREEGIKHGRTRPVSIHKIKAVVSNGDVVGYEHRMACQEMDLRHG
                     LGDVATGYITEYNNEGACQYFFAHTQKLFYKTGPTSITLKQKLLAKPTAAWRVVYSGQ
                     VHAADEIVIDELARMMGKDEVEYRLATLDSPRHVAVLEKAAQAANWGQKLPPGVAQGV
                     GMHDEYKSIAAYIMTIDTRGREPRMTACHIAVDNGHCVNPEGTKSSMLGQAHDGFALA
                     FRAGLHVDNGATRESNFHDYKWTRMFDSAPEMSVSILPNSNVLPGGIGELGVPAAAAA
                     AANAWARATGKQPRNFPLNEYGA"
     gene            1256..1717
                     /locus_tag="VHH53_13160"
     CDS             1256..1717
                     /locus_tag="VHH53_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2291110.1"
                     /translation="MPTITFNLNGNPVSVDADNDEELLWVLRDRMDVKGLKYGCGMGL
                     CGACTSHINGKAQRTCITPVSSIQGKNVTTIEGLAKGDQLHPVQQAWIDNDVAQCGYC
                     QAGQIMQAVSLLNSNPHPTDADIDEAMGGNLCRCGTYFRIRSAVKQVAAGR"
BASE COUNT          335 a          603 c          545 g          273 t
ORIGIN      
        1 cggacgccca gatcgacgac ctgctcaacg ggatcatcga cgacctcagc aacccgggcg
       61 agggcatcga ggccaccttc cgttggccgt acgtgccgca cgccccgatg gaggagaacg
      121 ccgccgtcgc caacgtcacc tctgacaagg ctgaggtgtg gggcggggcg cagattcctc
      181 ccacgctgca gcgtcagctg gccgagaccc tcggcatgcc catcgagaag gtcacgtacc
      241 acgtgatccg ggccggtggc gccttcggtc gtcacctgtt ccacgaccag gacgtccacg
      301 ctgcgcaggt ttcgcagcgc ctcggcaagc cggtcaagct gcagtggctg cgtgaggaag
      361 gcatcaagca cggccgtacc cgcccggtct cgatccacaa gatcaaggct gttgtgagca
      421 acggcgacgt ggtcggctac gagcaccgca tggcttgcca ggagatggac ctccgccacg
      481 gcctcggtga cgtcgccacc ggctacatca ccgagtacaa caacgagggt gcgtgccagt
      541 acttcttcgc tcacacccag aagctgttct acaagaccgg cccgacgagc atcaccctga
      601 agcagaagct cctggccaag cccaccgccg cctggcgtgt ggtgtactcc ggccaggtcc
      661 acgccgccga cgagatcgtc atcgacgagc tcgcccggat gatgggcaag gacgaggttg
      721 agtaccgcct cgccacgctc gacagcccgc ggcacgttgc ggtgctggag aaggccgccc
      781 aggcagccaa ctggggccag aagctgccgc cgggtgttgc ccagggcgtc ggtatgcacg
      841 acgagtacaa gtcgatcgcc gcgtacatca tgacgatcga cacccggggt cgtgagccgc
      901 ggatgacggc ctgccacatc gccgtcgaca acggccactg cgtgaacccc gaaggcacca
      961 agtccagcat gctcggtcag gctcacgacg gtttcgccct ggctttccgt gccggcctgc
     1021 acgtcgacaa cggcgccacc cgtgagtcga acttccacga ctacaagtgg acccgtatgt
     1081 tcgactcggc gccggagatg agcgtctcga tcctgccgaa cagcaatgtc ctgccgggtg
     1141 gtatcggtga gctgggtgtc ccggctgccg ctgctgccgc tgcgaacgcc tgggcccgtg
     1201 ctacaggcaa gcagccccgg aacttcccgc tcaacgagta cggagcctga tcgccatgcc
     1261 cacaatcacc ttcaatctca atggcaaccc ggtttcggtc gacgccgaca acgacgagga
     1321 actcctgtgg gttcttcgtg accgcatgga cgtcaagggc ctgaagtacg gatgcggtat
     1381 gggcctctgt ggtgcctgca ccagccacat caacggcaag gcgcagcgga cctgcatcac
     1441 gcccgtcagc tccatccagg gcaagaacgt caccacgatc gagggcctgg ccaagggtga
     1501 ccagcttcac cccgtccagc aggcctggat cgacaacgac gttgcccagt gcggttactg
     1561 ccaggccggc cagatcatgc aggccgtgtc gctcctgaac agcaacccgc acccgaccga
     1621 cgccgacatc gacgaagcga tgggcggaaa cctctgccgg tgcggtacct acttccgcat
     1681 ccggtccgcc gtcaagcagg tcgccgccgg taggtaggcg atttggagac gctctcccag
     1741 gtgggtcgcc ggagct
//
LOCUS       DATADN010001022         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1487945, whole genome shotgun sequence.
ACCESSION   DATADN010001022 DATADN010000000
VERSION     DATADN010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487945"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..644)
                     /gene="smpB"
                     /locus_tag="VHH53_13165"
     CDS             complement(168..644)
                     /gene="smpB"
                     /locus_tag="VHH53_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX2291111.1"
                     /translation="MSKERGRKVIVSNRRARHDYAIMDVYEAGIVLTGTEVKSLRAGR
                     ASLADAYASVEDGEVWLRGVHIPEYDQGSWTNHEPRRTRKLLLHRKEILRLIGKTREG
                     GLSLVPLSMYFADGKVKVEIALARGKRSYDKRADLAKRDADREVQRALGRRAKGMN"
     gene            complement(654..1556)
                     /gene="ftsX"
                     /locus_tag="VHH53_13170"
     CDS             complement(654..1556)
                     /gene="ftsX"
                     /locus_tag="VHH53_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX2291112.1"
                     /translation="MRASFVFSEVVTGLRRNVTMTMAMILTTAISLGLLGGGLLVVRT
                     IDQMQRDYYQRVETVVYLSADISANDPGCTSSTCAALRGELEAASGVQSVLFENRQAA
                     YARFQRIFEGQPELLQLARPEALPASLRVQLSDPQRFPSIAKSFNGRPGVDRVTDQGE
                     YLRQLFRALNGLRNATFVIAVIQALAALLLISNTIQVSAFTRRAEVGVMRLTGATRWY
                     TQLPFLLEAVVAGVIGGLLAIAGLVIGKVAFIDRVLAGVFEAAIVPRLGYADIFAVAP
                     ILLLVGGVVAALTGYVTLRLYVRA"
     gene            complement(1608..>2001)
                     /locus_tag="VHH53_13175"
     CDS             complement(1608..>2001)
                     /locus_tag="VHH53_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006349025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2291113.1"
                     /translation="NVAFALEVIGKPRHSIRKVVPEVLELVGLEGKADRMPHELSGGE
                     QQRVAIARAFVNRPLLLLADEPTGNLDPDISQDIMMLLERINRTGTTVLMATHDHSIV
                     DSMRRRVVELQLGQLVRDDSRGVYGVGH"
BASE COUNT          366 a          679 c          630 g          326 t
ORIGIN      
        1 gccagcgccg acagcagcgt agtcacggtc tgagccgacc tagccgggct gaggcgacgt
       61 cactgtccac tgacacgccg tgcaccagag catgacacac cgtgcaggag agccggacac
      121 gccggacatg ggaagcggac acgcccgggg aaatgccggg gcgacggtca gttcatgcct
      181 ttggcgcgcc gtcccagggc acgctgcacc tcgcggtcgg cgtcacgctt ggccaggtcg
      241 gcccgcttgt cataggagcg cttaccgcgg gccagcgcga tctccacctt caccttgccg
      301 tcggcgaagt acatcgacag cggcaccagt gacagcccgc cctccctggt cttaccgatc
      361 agccggagga tctccttgcg atgcagcagc aacttgcggg tccgccgcgg ctcgtggttg
      421 gtccagctac cttggtcata ttccggaatg tgcacgccgc gaagccacac ctccccgtcc
      481 tccacgctgg cgtaggcgtc ggccagcgaa gcgcggcccg cccgaagtga cttgacttcg
      541 gtgccggtga gcacgatacc cgcctcgtag acgtccatga tcgcgtaatc gtgcctggcc
      601 cgccggttcg acacgattac tttgcggccg cgctccttgc tcatacctcc tgctcaagcc
      661 cggacgtaca ggcgcaacgt cacgtaaccc gtcagtgcag ccaccacccc gcccaccagc
      721 agcagaatcg gcgcgacggc aaagatgtcc gcgtagccca accgcggcac gatcgcggcc
      781 tcgaacactc cggcaagcac ccggtcgata aaggcgacct taccgatcac caggcccgcg
      841 atcgccagta gaccaccgat cacgccggcg accactgctt cgaggaggaa gggaagctgg
      901 gtataccagc gggtggcgcc ggtcaggcgc atgacaccga cctcagcgcg ccgggtgaac
      961 gccgacacct ggatggtatt ggagatcagc agcagcgcgg cgagggcctg aatcactgcg
     1021 atcacgaaag tggcgttgcg caggccgttg agtgctcgga acagttgccg gaggtactca
     1081 ccctggtcag tgacccggtc cacgccaggg cgcccgttga acgacttggc gatggacggg
     1141 aagcgttgcg gatcggacag ctgcactcgt agcgacgccg gcaacgcctc gggtctagcc
     1201 agctgcagca gctcgggctg gccctcgaag attcgctgga aacgcgcata ggcggcttgc
     1261 cggttctcga acagcaccga ctgcacgccc gaggcggcct ccagttctcc acgcagcgca
     1321 gcgcacgtgc tggaggtgca gccaggatcg ttcgcactga tgtcggcgct gaggtacacg
     1381 acagtctcta cgcgttggta gtagtcacgc tgcatctggt cgatagtgcg gaccacgagc
     1441 aggcctccac ccagcaggcc cagggagatc gcagtggtca ggatcatcgc catggtcatg
     1501 gtgacgttgc ggcgcaggcc ggtgacgacc tcgctgaaca cgaagctggc acgcatcaga
     1561 agtggacatt cctcatctca gtgatcggca cctcagtgat cggcacctca gtggccgacg
     1621 ccgtacaccc cgcggctgtc gtctcggacc agttgcccga gctgcaactc cacgacgcgg
     1681 cggcgcatcg agtcaacgat cgagtggtca tgggtggcca tcagtaccgt ggtgccggtt
     1741 cggttgatcc gctccagcaa catcatgatg tcctggctga tgtcgggatc caggtttcca
     1801 gtgggctcgt cggccagcag cagcagcggt cggttcacga aggctctggc gatcgcgacc
     1861 cgctgctgct ccccgccgga cagctcatgt ggcatccggt ccgccttgcc ctccaagccg
     1921 accagctcga gcacctccgg caccaccttg cggatgctgt gccggggctt gccgatcacc
     1981 tcgagggcaa acgcgacgtt c
//
LOCUS       DATADN010001023         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1188453, whole genome shotgun sequence.
ACCESSION   DATADN010001023 DATADN010000000
VERSION     DATADN010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188453"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VHH53_13180"
     CDS             <1..704
                     /locus_tag="VHH53_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291114.1"
                     /translation="YFYNWSFSHVRTRYALKWDGDMVLTPQGEQVLRDLAWQLQGIDG
                     TVKMRRAPVYVESDRVAHVDLARTPGAGELWGWRNTPDHTFIKCFDWEFVRPWADYLL
                     EVPDWVCFELKWLDQDQFSHWSHTDFQAHRLSRKHREWEVNRALLDGTAPPEGVVRIE
                     SRDGTHVIDHLRQIYVALHRYGPTAPPPGDLVEVGDVRSSPADGRGGESALLPAQDAV
                     TVSQPTQQDEGIPHE"
     gene            697..1734
                     /locus_tag="VHH53_13185"
     CDS             697..1734
                     /locus_tag="VHH53_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2291115.1"
                     /translation="MSRDLLLVAGSGRSGTSLFVNIVGNLGFHVPRPWVATDNSNPRG
                     FGESQWVVDRHTALLRQANVQVTDARPTAWADAARVCFDEQVNAQVHRWLQDQFSRHD
                     QVVIKDPRLLWFLPLWNQVGSELDATVHVVTMLRHPAEVVSSKLQWYPNMPLVDANRV
                     AGWINTMLFTERATRDHRRAFVRFEDLLEDWTQQIARISAQLDVLSLVNARARDQASA
                     DSVVDRSLYRSKATWENLDVPEGLVRLAEQVWEDLLPLAGAGDAALAPLDKHREEYVS
                     YYRHAEHVAQSTTLAAIRQLRQGDPSKPPIAQPEPPANSLLSRVRRLIPARVRRLIPL
                     RLKRALMELVG"
     gene            1769..>2157
                     /locus_tag="VHH53_13190"
     CDS             1769..>2157
                     /locus_tag="VHH53_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291116.1"
                     /translation="MTTHNREGLEEYAIRWNWVSAPSDSAFTAVLRVKDEAHSLPWVL
                     PGVLRSVEQTIVVDNGSTDGTAEVARRVAEEVGLEERLQVLSYPFAVSRCGPEHLRTF
                     PDSVHSLTYFYNWSFSHVRTRYALKWDG"
BASE COUNT          350 a          706 c          736 g          365 t
ORIGIN      
        1 cctacttcta caactggtct ttttcgcacg tgcgcacgcg gtatgcgctg aagtgggatg
       61 gggacatggt gctcaccccc cagggcgagc aggtgctgcg tgacctcgcc tggcagctcc
      121 agggcatcga cgggacggtg aagatgcgcc gtgcgccggt ctacgtcgag agcgaccggg
      181 tcgcgcatgt ggacctcgcg cgcactcctg gcgctggcga actctggggc tggcgtaata
      241 cccccgacca cacgttcatc aagtgcttcg actgggagtt cgtgcggccc tgggccgact
      301 atctcctcga ggtgccagac tgggtatgct tcgagctgaa gtggctcgac caggaccagt
      361 tcagtcactg gtcccacacc gacttccagg cgcaccgcct ctcccgcaag caccgtgagt
      421 gggaggtgaa ccgcgcgctc ctcgatggca ccgcaccccc agagggggtc gtccggatcg
      481 agagtcgcga cgggacgcac gtcatcgacc acctccggca gatctacgta gcgttgcacc
      541 ggtacggccc gaccgcaccg ccccctggcg acctcgtcga ggtcggggac gtgagatcat
      601 caccggctga cggccgcggc ggcgaatcgg cgctgctgcc tgcgcaggat gcggttacgg
      661 tttcgcaacc gacccagcag gacgagggta tcccgcatga gtagagatct gctgctcgtg
      721 gctggttccg gccgtagcgg caccagcctg ttcgtgaaca tcgtcggtaa cctcggcttc
      781 cacgtgccca ggccgtgggt ggcgaccgac aatagcaacc cacgaggttt cggcgagtcg
      841 cagtgggttg tggacaggca caccgcgctc ctgcggcagg cgaacgtgca agttaccgat
      901 gcccgcccga ccgcctgggc tgacgctgcc agggtctgct tcgacgagca ggtcaacgcc
      961 caggtgcacc gttggttgca ggaccagttc tcccgccacg accaggtggt gatcaaagac
     1021 ccgcgactgc tgtggttcct gccgctgtgg aaccaggtcg gcagcgaact ggacgccacc
     1081 gtgcacgtgg tcaccatgct ccggcatcct gccgaggtgg tgagcagcaa gctccagtgg
     1141 tacccgaaca tgcccctggt cgacgccaac cgcgtggccg gctggatcaa cacgatgctg
     1201 ttcaccgagc gggcgacgcg cgaccaccgg cgggccttcg ttcggttcga ggacctgctg
     1261 gaggactgga cccagcagat cgcgcgcatc agcgcacagt tggacgttct gtccctggtt
     1321 aacgcgcgag ctcgtgacca ggcgtcggcc gacagcgtcg tcgaccgttc gctgtaccgc
     1381 tcgaaggcaa cctgggagaa cctcgacgtc cccgaggggc tggttcgcct cgccgagcag
     1441 gtgtgggagg acctgctgcc gctcgccggc gccggtgacg cggcgctggc tcctctggac
     1501 aagcaccgcg aggagtacgt atcctactac cgccacgccg agcacgtcgc ccagtcgacc
     1561 accctggccg cgatccggca actgcgccag ggggacccgt ccaagccgcc catagctcag
     1621 ccggagccgc cggcaaactc cttgctgagc cgggtgcggc ggctgatccc ggccagggtg
     1681 cggcgcctga tccctctccg gctaaagcgg gccttgatgg agctggtcgg atgatgtgtc
     1741 ccccacaggt agtagctgca gcgtggcggt gacgacacac aaccgggagg ggttggagga
     1801 gtacgcgatc cggtggaact gggtgagcgc tccctccgat tcagcattca cggcggtgct
     1861 gcgcgtcaag gacgaggcgc actcgctgcc gtgggtgctg cccggggtgc tgcgctcggt
     1921 ggagcagacg atcgtcgtgg ataacggctc caccgacggc acggcggagg tggcccgccg
     1981 ggttgcggag gaggtgggtc tggaggagag gctgcaggtg ctctcctacc cgtttgcggt
     2041 ctcgcgctgc ggacccgagc acctgcggac gttcccggat tcggtgcaca gcctgaccta
     2101 cttctacaac tggtcttttt cgcacgtgcg cacgcggtat gcgctgaagt gggatgg
//
LOCUS       DATADN010001024         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_109732, whole genome shotgun sequence.
ACCESSION   DATADN010001024 DATADN010000000
VERSION     DATADN010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109732"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VHH53_13195"
     CDS             complement(<1..1186)
                     /locus_tag="VHH53_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291117.1"
                     /translation="MMLRRAVSRARGGMLAIAAVLTLAAALSVVGVLGGQTLPQRSGP
                     PIESLAVERAALSPGTIELTLRNTGPDPVQVGQVAVNDSYVAFTGGEEPIGRLDAGTL
                     RLDVPWQDGQPYLVSILTSTGAVIEHEIPVAAMTPQPDGKSVGLMVLLGTYVGIIPVL
                     LGMLFLPAVRRVNAQTVRVLLALTVGLLAFLAADAALEAIGLAGQTGAAFGGVALVLL
                     GTGLAFLALTSVDRFLQARQDRAGCGSAAGSPDGQRLALMIAIGIGLHNLGEGLAIGS
                     AYAIGALAIGGSLVIGFAIHNTTEGLAVVVPLAGRPVSLLRLLGLGLLAGAPAVAGTL
                     IGSTVVNAELSAFLLGIGVGAILQVIVQITPSLRDRASGLIDPATYGGLAAGALVMYA
                     TSL"
     gene            complement(1197..2279)
                     /locus_tag="VHH53_13200"
     CDS             complement(1197..2279)
                     /locus_tag="VHH53_13200"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2291118.1"
                     /translation="MTDPAHPQTSRRWFLAGSALVGTAGAALGTSALLGSPAGAISNG
                     DTAGHLHPTLGAGHGTHSGGLAGPTFRAGRSVDHEANGFNPTELLRDFDAGTTSRLPD
                     GRTLREWEIFATDHEIEVAPGVRFPAWTFNARVPGPTLRATAGDVLRVRFVNGSAHPH
                     TMHFHGIHPAEMDGVPGIGAGLIEPGASVTYSFDAEPFGLHLYHCHVGPLAEHIARGM
                     YGTFLIDPPQPRPAADEMVMVMHGYNTTFDGQGNQLYAVNGIPFHYMHEPVRVRRGEL
                     VRIYLVNILEYDPINSFHLHGNFFEYYPTGTRLQPAEYTDTVVLGQGQRGICELRFPH
                     VGRYMFHAHKTEFADLGWMGFFEVVE"
     gene            complement(2276..2962)
                     /locus_tag="VHH53_13205"
     CDS             complement(2276..2962)
                     /locus_tag="VHH53_13205"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX2291119.1"
                     /translation="MSDRPACCGLTHTTAVEDALRTIFVRSGRGEPISTSAIASHLGL
                     TPPTVSTMLKRLTEHGLLERTDDHRALLTQHGRKHAQEVVRRHRLLETFLVTVLDLNW
                     DEVHDEADVLEHAVSDRLVDRIDEVLGHPTHDPHGDPIPPPAGRHDEGWGVRLDSVAT
                     GSRFRVERVYDRDSAALRYLAGLGVRPGVTIKVGERSPFGGPLWIELNGQRCALGDPL
                     VCLVHGREER"
BASE COUNT          518 a         1124 c         1082 g          471 t
ORIGIN      
        1 acaggctggt ggcatacatc accaacgcac ctgcggcgag gccgccgtag gtggcgggat
       61 cgatcaggcc gctggcccgg tcccgcaggc tgggcgtgat ctgcacgatc acctggagga
      121 tcgcgccgac cccgatgccg agcaggaacg cggacagttc ggcgttgacg actgtggaac
      181 cgatcagcgt cccggccacg gcgggtgcac cggccagcaa acccagcccg aggaggcgca
      241 gcagcgacac cggccgcccg gccagcggca ccaccacagc caggccctcg gtggtgttat
      301 ggatggcgaa gccgatcacc agcgatccgc cgatggccag cgcaccgatc gcataggccg
      361 acccgatggc gagcccctcg ccgaggttgt gcaacccgat tccgatcgcg atcatcagcg
      421 cgagccgttg gccgtccgga gatcccgccg cggacccgca cccggcccgg tcctggcggg
      481 cttgcaggaa ccggtcaacg gaggtcaatg ccaggaaagc gaggccggta ccgagcagga
      541 cgagcgcgac gccgccgaac gcagcaccgg tctgcccagc gagcccgatt gcctcgagcg
      601 cagcgtccgc ggccagaaac gccagcaacc cgacggtcag cgccagcagc acgcgcaccg
      661 tctgcgcgtt cacccggcgc actgccggca ggaacagcat cccgagcaga acggggatga
      721 tcccgacata agtaccgagc agcaccatca gcccgacgga tttgccgtcc ggctgaggcg
      781 tcatcgccgc caccggaatc tcgtgctcga tcaccgcgcc cgtcgaggta aggatcgaga
      841 ccagatacgg ctggccgtcc tgccatggca cgtcgaggcg aagcgtcccg gcgtcgagtc
      901 gcccgatcgg ctcctcgccg ccggtgaacg ccacataaga atcgttgact gcgacctggc
      961 cgacctgcac cggatcgggc ccggtgttgc gcagggtcag ctcgatggtc ccaggcgaca
     1021 gcgccgcccg ttcgacggcc aggctctcga tcggtgggcc gctgcgctgc ggcagcgtct
     1081 gcccgcccag cacgcctacc acggacagcg cggccgcgag ggtcagcacc gctgcgatcg
     1141 cgagcatccc gccgcgagcg cggctcaccg cacggcgcag catcaccggt ttcccttcat
     1201 tcgacgacct cgaagaaccc catccagccc agatcggcga actcggtctt gtgcgcatgg
     1261 aacatgtatc gcccgacgtg cgggaatcgc agttcacaga tgccgcgctg accctggccg
     1321 agcacgaccg tgtcggtgta ctcagcaggc tgcagccggg tgcccgtcgg gtagtactcg
     1381 aagaagttcc cgtgcaggtg gaagctgttg atcgggtcgt actccagaat gttcaccagg
     1441 tagatccgga cgagctcacc gcggcgcacc cggaccggct cgtgcatgta atgaaacggg
     1501 atgccgttga ccgcgtagag ctggttgccc tggccgtcga acgtggtgtt gtagccgtgc
     1561 atcaccatca ccatctcgtc ggccgcgggc cgcggctgcg gcgggtcgat gaggaacgtg
     1621 ccgtacatcc cgcgggcgat gtgttcggcc agcgggccga cgtggcagtg gtagaggtgc
     1681 agcccgaacg gttcggcgtc gaaggagtag gtgacgctgg cgccaggttc gatgagccca
     1741 gcaccgatcc cgggcacccc atccatctcc gccggatgga tcccgtggaa gtgcatcgta
     1801 tgcggatgcg ccgagccgtt cacgaaccgc acccgcagca cgtcacctgc cgtggcgcgc
     1861 agggtcggtc cgggcacccg cgcgttgaaa gtccacgccg ggaaccggac gccgggcgcc
     1921 acctcgatct cgtggtcggt ggcgaagatc tcccattcgc gcagcgtccg cccgtccggt
     1981 agccgtgacg tggtgccggc gtcgaagtcg cgcagcagtt ccgtcgggtt gaacccgttg
     2041 gcctcgtgat cgacgctgcg accggcgcgg aatgtcggac cggccaaccc gccgctgtgc
     2101 gtgccgtgcc ctgctccgag cgttgggtgc aggtgtccgg ccgtatcccc attgctgatt
     2161 gcgccagcgg ggctcccgag cagtgcgctg gttcccagtg ccgcgccagc ggtaccgacc
     2221 agcgcgctgc cggcgaggaa ccaccgcctg gacgtctgtg ggtgggcagg gtcggtcaac
     2281 gctcctcccg gccgtgcacc aggcacacga gcgggtcgcc gagcgcgcag cgctgcccgt
     2341 tcagctcgat ccagagcggg ccaccgaacg ggctgcgctc ccccaccttg atggtcacgc
     2401 ccggtcggac gccgaggcca gccaggtagc gcagcgcagc gctgtcccgg tcatacaccc
     2461 gctcgacgcg aaaccggctg cccgttgcca ccgaatccag ccgcacgccc cagccctcgt
     2521 cgtgccggcc tgccggcggc gggatcggat cgccgtgcgg gtcatgggtg ggatgcccga
     2581 gcacctcgtc gatgcggtcg acgagccggt cggacaccgc gtgctcgagc acgtcggcct
     2641 cgtcgtgcac ctcgtcccag ttcaggtcga ggaccgtgac caggaacgtc tccagcaacc
     2701 ggtgccgccg gacgacctct tgggcgtgct tgcggccgtg ctgggtgagc agcgcgcggt
     2761 ggtcgtcggt gcgctcgagc agcccgtgct cggtcagccg cttgagcatg gtcgacaccg
     2821 tcggcggcgt gaggcccagg tgagaggcga ttgccgaggt gctgatcggc tcgccacggc
     2881 cggagcgcac gaagatcgtt ctcagcgcgt cctctaccgc cgtggtgtga gtcaacccgc
     2941 aacaggccgg ccgatcggac attcgatgcc tctcacgctt ggagcaagag tgggcgaatc
     3001 tacctgcgca gggtaaggag gccgccgacg gatggccgtc tcatcttgag agaccgacgc
     3061 cccgttggcc tatcgtggct gccgcacttc acctgtccgc cgggtctgcc ccgcacggcg
     3121 ccgggcatgc cccatcgcac atcagtcgct cgaccgtgca ccgagctacc ggttgagctg
     3181 gtccagatcg cttcc
//
LOCUS       DATADN010001025         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_728145, whole genome shotgun sequence.
ACCESSION   DATADN010001025 DATADN010000000
VERSION     DATADN010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728145"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VHH53_13210"
     CDS             <1..387
                     /locus_tag="VHH53_13210"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX2291120.1"
                     /translation="RLAGLTAPALAPAVADVRDQLACLIRPGFVTATGAQRLPDLARY
                     LQAIERRLDKLPRDPDRDRNWMQRVQTVVQAYQQLRSEVGDGPQLRHIYWMIEELRVS
                     YFAQELRTPYPVSDKRIYQAMDELAG"
     gene            complement(406..1365)
                     /locus_tag="VHH53_13215"
     CDS             complement(406..1365)
                     /locus_tag="VHH53_13215"
                     /inference="COORDINATES: protein motif:HMM:NF041045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2291121.1"
                     /translation="MSVARPERTGLVHEALFYLDEQDYANGIARFVRDGFELAEPALV
                     AVPGSHLDVLRSVLGPDADRVRFVDMAEAGRNPGRIIPTVFYAFAHQHSTSRVRIVGE
                     PIWPGRSAAEYRAALQHEDLLNVALAGQQATILCPYDRQGLDIAVLDDARRTHPGLVA
                     GGQREISSDYADPRAVADASLRLLPDPPEWWGDMRVFSSLTDLRAIRQFVEGLALRAG
                     LGAQRVSDLCLAVDEVATNTLQHTHAAGVLALWQDPDTESLVCQITDSGQLHDRLVGR
                     MPPGEGKSHGRGLILVNALCDLVEMPTGQIGVGTTVRLHMRLR"
     gene            1631..2779
                     /locus_tag="VHH53_13220"
     CDS             1631..2779
                     /locus_tag="VHH53_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2291122.1"
                     /translation="MRRRLFEDEHDAFRDSVRTFVAKEVLPHCAEWEAQGIVPRELFL
                     RAGSSGFLGMAVPEEFGGGGAADFRFNAVLGEEVMQPGAAAAGLGLTLHNDICLPYFL
                     DLATEEQQRRWLAGIAAGQLITAIAMTEPGMGSDLASMATTAVRRSDHYLVNGTKTFI
                     TNGINADLVITAVKTDLQQRHQGISLIVLERGMDGFERGRNLAKLGQHSQDTAELFFR
                     DVKVPITNLLGEPGRGFTHLVDRLPQERLSIAISGVAGARTALDLTLAYVKDRKAFGQ
                     PIGSFQNTRFRLAEMVTEIDLAQTFIDRCIEALNDGELTVADAAKAKWWCTELQGRVV
                     DACLQLHGGYGYMLEYPIARAYADARITRIYGGTTEIMKEIIGRSLGL"
BASE COUNT          538 a          931 c          982 g          500 t
ORIGIN      
        1 cggctggccg ggctcaccgc gccggcactg gcacccgcgg ttgctgatgt ccgcgaccag
       61 ctggcctgcc tgatacgccc cggcttcgtg accgcaacgg gcgcgcagcg gttacccgac
      121 ctcgctcgct acctacaggc catcgagcgc aggctcgaca agctgccccg cgatccggac
      181 cgggaccgga actggatgca gcgggtccag accgtcgtgc aggcgtacca gcagctgcgt
      241 agcgaggtcg gcgacgggcc gcagctacgg cacatctact ggatgatcga agagctgcgg
      301 gtcagctact tcgcccagga gctgcgtacc ccgtacccgg tctcggataa gcggatctat
      361 caggcgatgg acgagctggc cggctaactc acccagctaa cttactcagc gcagccgcat
      421 gtgcagccgg accgtggtgc cgacgccgat ctgaccggtg ggcatctcga ccaggtcgca
      481 cagcgcgttg accaggatca gcccgcggcc atgcgatttc ccttcaccgg gcggcatccg
      541 gccgaccaac cggtcgtgca actgcccgct gtcggtgatc tggcagacca ggctctcggt
      601 gtctgggtcc tgccacaagg ccagtacacc tgccgcgtgg gtgtgctgca gggtgttcgt
      661 ggcgacctcg tcgaccgcaa gacacagatc ggacacccgc tgcgcgccca gcccggctcg
      721 caacgccagg ccctcaacga actgccggat ggcccgcagg tcagtcaggg aggagaagac
      781 tcgcatgtcg ccccaccact ccggaggatc gggcaacaag cgaaggctcg cgtcggcgac
      841 ggcccgggga tcggcgtagt ccgagctgat ctctcgctgc cctcccgcga ccagtcccgg
      901 atgggttcgc cgggcgtcgt cgaggaccgc aatgtcgagt ccctgacggt cgtacgggca
      961 caggatagtg gcctgctggc ccgccagggc gacgttgagc aggtcttcgt gttgcaatgc
     1021 ggcgcggtat tcggcggcgg accgacctgg ccagatcggt tcgcccacga tgcgtacccg
     1081 actcgttgag tgctggtggg caaacgcgta gaacacggtg ggaataatcc ggccgggatt
     1141 gcgacccgcc tcggccatgt cgacgaaccg gacccggtcc gcgtcggggc ccaggaccga
     1201 gcgcagcacg tcgaggtgtg aacctgggac cgcgaccagg gccggttcgg ccaactcgaa
     1261 cccgtcccga acgaagcgcg cgatgccgtt ggcgtagtcc tgctcgtcga ggtagaacag
     1321 cgcctcgtgc accagcccgg tgcgctcggg ccgcgcaacg ctcatgggct gactcgccac
     1381 gttcatacgc tcgacctccg gtccctctat ggcgcagagg gtacaactgt tcgttgcgct
     1441 gaacatgtga aaatctcact tatcggtgag cgatcaatcc ggtactggca gtacgtaggt
     1501 atccatatgg cgcagcgagc gagtagtgag ttgtcgggcg gagtttaaca acttcgccga
     1561 accgccgctg gtgacgtggc ccacaggaca agcaccagca ggaggagcgc agcgggagga
     1621 gcagcggcac atgcgtcgtc ggttgttcga agatgagcat gacgcgttcc gggacagcgt
     1681 ccggactttc gtggccaagg aggtgttgcc acactgcgcg gagtgggaag cccagggcat
     1741 cgtgccgcgg gagctgttcc tgcgagccgg gtccagcggc ttcctcggta tggctgtccc
     1801 ggaggagttc ggcggcggcg gggcggccga cttccggttc aacgcggtgc tgggtgagga
     1861 ggtcatgcag cctggagcgg cggctgcagg gctcgggctg actctgcaca acgacatctg
     1921 cttgccttac ttcctggatc tggccaccga ggagcagcag cggcggtggc tggccggcat
     1981 cgccgccggg cagctgatca cggcaattgc gatgacagag ccgggaatgg gctccgatct
     2041 ggcgagcatg gccaccacgg cggtgcgccg atccgaccac tatctggtta acgggacgaa
     2101 gacattcatc accaacggca tcaacgctga tctggtcatc actgccgtga agaccgatct
     2161 gcagcagcgg catcagggga taagtctgat cgtactggaa cgcggaatgg acggtttcga
     2221 gcgcggtcgc aacctcgcca agttgggcca gcattctcag gacaccgccg aattgttctt
     2281 ccgcgacgtc aaggtaccga tcaccaacct gctcggtgag ccgggccgcg ggttcactca
     2341 cctagtggac cggttacccc aggagcggct gtcgatcgcg atctccgggg tggccggggc
     2401 gcggacagcg ttggacctga cgctggccta cgtcaaggac cgcaaagcct tcggtcaacc
     2461 gatcggatcc tttcagaaca ccaggttccg gctggccgaa atggtcaccg agatcgacct
     2521 cgcgcaaacc ttcatcgacc gctgcatcga ggcgctcaac gacggtgagc tgacggtggc
     2581 cgacgccgcc aaggccaagt ggtggtgcac cgagctgcag ggccgggtgg tcgacgcctg
     2641 cctgcagttg cacggcggct acggatacat gctcgagtat cccatcgcgc gggcctacgc
     2701 cgatgctcgc atcacccgaa tttatggtgg taccacagag atcatgaagg aaatcatcgg
     2761 ccggtcactt ggtctgtgat ccgcgaggcg agaatcgcga cagtgctgct catgtcgtat
     2821 ttcttgtcgc ctttgcctgg ctggagcaac tgctgcttca atctcggcta ccagggcgag
     2881 atcgggtgat gacaaaccga tcggtctgat acgcacagtg aggacaagct cggaagtgac
     2941 gcaagcttgc a
//
LOCUS       DATADN010001026         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1188670, whole genome shotgun sequence.
ACCESSION   DATADN010001026 DATADN010000000
VERSION     DATADN010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188670"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1696
                     /locus_tag="VHH53_13225"
     CDS             <1..>1696
                     /locus_tag="VHH53_13225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX2291123.1"
                     /translation="FFGTSQLSQFMDQTNPLAGLTHKRRLSALGPGGLSRMERVVRER
                     MTTQDVEAITPQTLINIRPVVAAIKEFFGTSQLSQFMDQTNPLAGLTHKRRLSALGPG
                     GLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLIGSLSSYARVNPFGFIETPYRKV
                     VDGLVTEQIDYLTADEEDRHVIAQANAVLAEDGHFAEDRVLVRRKGGDVDYIDPFGVD
                     YMDVSPRQMVSVATAMIPFVEHDDANRALMGANMQRQAVPLLRSEAPLVGTGMELRAA
                     VDAGDVVVADKAGVVEELCADYVTVMADDGTRQTYRMHKFHRSNQGTCTNQRPIVAEG
                     DRIEVGHVIADGPCTDSGEMALGKNLLVAIMPWEGHNYEDAIILSQRLVQDDVLTSIH
                     IDEHEIDARDTKLGAEEITRDIPNVSEEVLADLDERGIIRIGAEVQAGDILVGKVTPK
                     GETELTPEERLLRAIFGEKAREVRDTSLKVPHGETGKVIGIRVFSREDDDELSPGVNE
                     LVRVYVAQKRKIQDGDKLAGRHGNKGVIGKILPVEDMPFLTDGTPVDIILNTHGVPR"
BASE COUNT          310 a          540 c          570 g          276 t
ORIGIN      
        1 ttcttcggca cctcccagct gtcccagttc atggaccaga ccaatccgct ggccgggttg
       61 acccacaagc ggcggctgtc cgccctgggt cctggtggtc tgtcccgcat ggaacgggtc
      121 gtgcgcgagc ggatgacgac ccaggacgtc gaggccatca cgccgcagac cctgatcaac
      181 atccgaccgg tggtagcggc gatcaaggaa ttcttcggca cctcccagct gtcccagttc
      241 atggaccaga ccaatccgct ggccgggttg acccacaagc ggcggctgtc cgccctgggt
      301 cctggtggtc tgtcccggga gcgggctggc ttcgaggtcc gcgacgttca ccattcgcac
      361 tacggccgga tgtgcccgat cgagaccccg gagggcccga atatcgggct catcggctcg
      421 ctgtcctcct atgcacgggt caatccattc ggtttcatcg agacgcccta ccgcaaggtc
      481 gtcgacggtc tggtcaccga gcagatcgac tacctcaccg ctgatgagga ggatcggcac
      541 gtcatcgcgc aggccaacgc ggtgctggcc gaggacggcc acttcgccga ggaccgcgtg
      601 ttggttcggc gcaagggtgg tgacgtcgac tacatcgacc cgttcggcgt ggactacatg
      661 gacgtctcgc cgcggcagat ggtgtcggtg gccaccgcga tgatcccgtt cgtcgagcac
      721 gacgacgcca accgcgcact gatgggtgcc aacatgcagc gccaggcggt gccgctgctg
      781 cgcagcgagg cgccgctggt gggaaccggc atggagctgc gtgccgcggt ggatgctggc
      841 gacgttgtgg ttgcggacaa ggccggcgtg gtcgaggagc tgtgcgccga ctacgtcacc
      901 gtcatggccg atgacggcac gcggcagacc taccggatgc acaaatttca ccggtccaac
      961 cagggcacct gcaccaacca gcgacccatc gtggccgagg gggaccggat cgaggtcggt
     1021 cacgtgatcg ccgatgggcc gtgcaccgac tctggcgaga tggctctggg caagaacctg
     1081 cttgtggcga tcatgccgtg ggaggggcac aactacgagg acgcgatcat cctgagccag
     1141 cgcctggtgc aggatgacgt gctcacctcg atccacatcg acgagcacga gatcgatgcc
     1201 cgggacacca agctgggtgc tgaggagatc actcgcgaca tcccgaacgt ctccgaggag
     1261 gtgctggcgg atctcgacga gcgaggcatc atccggatcg gtgctgaggt acaggcggga
     1321 gacatcctgg ttggcaaggt gacgccgaag ggcgaaaccg agctgactcc cgaggagcgg
     1381 ttgctccgcg ccatcttcgg cgagaaggcc cgcgaggttc gcgacaccag cctcaaggtg
     1441 ccgcatggcg aaaccgggaa ggtcatcggc atccgggtgt tctcccggga ggacgacgat
     1501 gagctgtcgc ctggcgtcaa cgagctggtc cgggtctacg tcgcccagaa gcgcaagatc
     1561 caggacggtg acaagctcgc cggccggcac ggcaacaagg gcgtcatcgg caaaatcctg
     1621 ccggtggaag acatgccgtt cttgaccgac ggcacgcccg tggacatcat cctcaacacc
     1681 catggtgtgc cgcgcc
//
LOCUS       DATADN010001027         1597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1038161, whole genome shotgun sequence.
ACCESSION   DATADN010001027 DATADN010000000
VERSION     DATADN010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038161"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /gene="rplD"
                     /locus_tag="VHH53_13230"
     CDS             complement(<1..508)
                     /gene="rplD"
                     /locus_tag="VHH53_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX2291124.1"
                     /translation="MIEAPHYAADGAKRGSNFQLPAEYFDGTVNEPVLHQAVKVYLNN
                     QRQGTHMTKSRSFVSGGNQKPWKQKGTGRARQGSIRAPHWRGGGIVFGPTPRDYRTEI
                     PRKVRQLARRSALNARAREGALHVVERLAFRTPKTAQLAELLGSLGLAGRKALVLTAG
                     HNANAYLSG"
     gene            complement(501..1118)
                     /gene="rplC"
                     /locus_tag="VHH53_13235"
     CDS             complement(501..1118)
                     /gene="rplC"
                     /locus_tag="VHH53_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX2291125.1"
                     /translation="MGLIGRKLGMTRVFTEDGTAVPVTVIEAGPCRVVQVRDGGVQLG
                     FGARRAQRTTKAELGHAKKAGLDAAPQVLRSFTINGEAPAPGSEMKVDIFAPGELVKV
                     TGTTKGRGFQGVVHRHGFGGGPATHGNTRHRKPGSISPGTDPSRVIKGKRMPGHMGAE
                     RHTEVGLRVVRVDAERNLLFVRGAIPGSKNGIVTVAKQGGPSRHD"
     gene            complement(1127..1435)
                     /gene="rpsJ"
                     /locus_tag="VHH53_13240"
     CDS             complement(1127..1435)
                     /gene="rpsJ"
                     /locus_tag="VHH53_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX2291126.1"
                     /translation="MAQKIRIRLKAFDHVVLDQAAADIVRTAEKTGAQVSGPIPLPVK
                     TERWTVLRSPHVDKKSREQFELRTHKRLMDINDSRPQTMDALTKLDLPAGVDVEIKVE
                     "
     gene            complement(1459..>1597)
                     /locus_tag="VHH53_13245"
     CDS             complement(1459..>1597)
                     /locus_tag="VHH53_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX2291127.1"
                     /translation="MVMPGDNTKMVVELITPVAVEDGLRFAIREGGRTVGAGVVTKIL
                     K"
BASE COUNT          237 a          567 c          487 g          306 t
ORIGIN      
        1 gaccactgag gtacgcgttg gcattgtgcc ccgccgtcag caccagcgcc ttccgtccgg
       61 ccagacccag gctgccgagg agttcggcca gctgggcggt ctttggcgtg cggaacgcga
      121 gccgctccac cacgtgcagc gcgccttcgc gggcccgtgc gttgagagcg ctccgccggg
      181 cgagctgccg caccttccgc ggaatctccg tccggtagtc acgcggcgtg ggaccgaaca
      241 cgatgccgcc accgcgccag tgcggggcgc gaatcgagcc ctgccgggcc cgcccggtgc
      301 ccttctgctt ccagggcttc tggttgccac ccgacacgaa gctgcggctc ttggtcatat
      361 gggtgccctg acgctggttg ttcaggtaca ccttgaccgc ctggtgcagc accggctcgt
      421 tcacggtgcc gtcgaagtat tcggcgggca gctggaagtt cgacccgcgc ttggcgccgt
      481 ccgcggcgta gtggggagcc tcaatcatgt cggctcggcc ctccctgttt ggctacagtc
      541 acgatgccgt tcttcgatcc gggaatggcc ccgcgaacaa agagcagatt gcgctcggcg
      601 tccacccgga ccactcgaag accgacctcg gtgtggcgct ccgctcccat atgccccggc
      661 atccgctttc ccttgataac tcgcgacgga tccgtgcccg gactgatcga gccaggcttc
      721 ctgtgccggg tgttgccgtg ggtggccggc ccaccgccga acccgtgccg gtgtaccacc
      781 ccctggaatc cgcggccctt ggtggtgccg gtgaccttca ccagttcacc aggagcgaag
      841 atgtccacct tcatctcgga cccgggggca ggagcttcgc cgttgatcgt gaagctccgg
      901 agtacctggg gcgcggcatc caaaccggcc ttcttggcat gacccagctc cgccttcgtc
      961 gtgcgctggg cgcgccgggc gccaaagccc aactgaacgc cgccatcgcg aacctgcacc
     1021 acccggcagg gcccagcctc gatcaccgtc accgggacag ctgtcccatc ttcggtaaac
     1081 acccgggtca tgccgagctt gcggccaatc aatcccatac ccatcgctat tcgaccttga
     1141 tctcgacgtc cacgccggcc ggcagatcca gcttggtcag cgcatccatc gtctgcggcc
     1201 gggaatcgtt gatgtccatc agccgtttat gcgtgcgcag ctcgaactgc tcccggctct
     1261 tcttgtcgac gtgggggctt cggagcaccg tccaccgctc ggtttttacc ggcagaggga
     1321 tgggacccga cacctgagcg ccggtctttt ccgcggtgcg cacgatatcg gccgcggcct
     1381 ggtcgagaac cacgtggtcg aaggccttga ggcggattcg gattttctga gccattgatt
     1441 cctcgctaat gctcgtcgct atttcaggat cttcgtgacc acgccggcgc ctacggtgcg
     1501 gccgccctcg cgaatcgcga agcgcagtcc gtcctccacc gctaccggcg tgatcagctc
     1561 caccaccatc ttggtgttgt cgccaggcat caccatc
//
LOCUS       DATADN010001028         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1319989, whole genome shotgun sequence.
ACCESSION   DATADN010001028 DATADN010000000
VERSION     DATADN010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319989"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1752)
                     /locus_tag="VHH53_13250"
     CDS             complement(133..1752)
                     /locus_tag="VHH53_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291128.1"
                     /translation="MATPNIEAIIRDRVTLTVDCIDRLYLNGYVPKLQTSGQLCWFLQ
                     EHLGNPIPSPALLRPLHDRFVREVSAFADAGKIPVVHFERGQRKDDIAAEHRTRYSGA
                     EGVVFIGVAQERASAFKARKLTGSERGVLFEFSRQSVAVNHYYFYVQDREWGPAFVKV
                     GTYLPYPVRVCLNGHEWAKQQAQQAGLDFTSLDNGFLACDDPARLQAICDGLGPADVQ
                     RFFDRWQAQLPWPLTVADRAAGYQHRLSIWQLEVSRTQVFDRPVQGRHFFEAVIRENL
                     DLGRPDRVSLLFPTRLTRRTPPPAHGYRTRVITTGIAPSLHVAYKHTDVKQYFKEERA
                     LRTETTINDPTDFQSKKALDTLPQLRATGAQINQQLLTVERVSHACSLSQDTLAQLQT
                     PSQQDGQRVPALRFGDPRVVALLQALCRFAQLPAGFRNRDLRPHVAALLGRALTTYSR
                     GAMTYDLRRLRLHGLIERVPRTHRYTVTSFGLRVAFFCSKIHLRILRPASAALVDPPD
                     DLPHPLRDALRLLDQAIDQLCAAAQLHPEAA"
BASE COUNT          299 a          600 c          710 g          315 t
ORIGIN      
        1 tccccgctcc gcccgcgcac cccgatggtg ccatccatcc gccgggagca ctccctatgg
       61 cggggccgct tgacgcccga ataccgtgac tagccggctc aaagccgtga cttgcggacg
      121 aatgagtcac gtttaggcgg cctccgggtg gagttgtgcg gcggcgcaga gttgatcgat
      181 ggcctgatcg aggagtcgga gggcatcgcg gagcggatgc gggaggtcgt cggggggatc
      241 gacgagggcg gccgaggccg ggcgcaggat gcggagatgg atcttggagc agaagaaggc
      301 gacgcgcaga ccgaagctgg tgacggtgta gcggtgggtc cggggcacgc gctcgatcag
      361 gccatgtagg cggaggcgcc gcagatcgta ggtcatcgcg ccgcgcgagt aggtggtgag
      421 ggcgcggccg agcagggccg cgacgtgcgg tcggaggtca cggttgcgga agccggccgg
      481 gagctgagcg aagcggcaga gcgcctggag gagtgccacg acgcgcgggt cgccgaagcg
      541 cagggccggg acgcgctggc cgtcctgctg gctcggggtc tggagttggg cgagggtgtc
      601 ctggctgagc gagcaggcgt ggctgacgcg ctcgacggtc aacagttgct ggttgatctg
      661 ggcgccggtt gcgcgcaact gggggagggt gtcgagggcc ttcttggact ggaagtcggt
      721 cgggtcgttg atggtggtct cggtccgcag cgcccgctcc tccttgaagt actgcttgac
      781 gtcggtgtgc ttgtacgcga cgtgcaggct gggcgcgatc ccggtggtga tgacgcgggt
      841 ccggtagcca tgcgctggcg gcggagtccg gcgggtcaac cgggttggga acagcaggct
      901 gacccggtcg ggccggccaa ggtcgaggtt ctcgcggatc accgcctcga agaagtggcg
      961 gccctggacc ggccggtcga agacctgggt ccgactgact tcgagctgcc agatgctcag
     1021 ccgatgctgg tagccggcgg cccggtcggc aacggtgagg ggccagggca actgcgcctg
     1081 ccagcggtcg aagaaccgct gcacgtcagc cggcccgagc ccatcgcaga ttgcctggag
     1141 gcgggccggg tcgtcgcagg ccaggaagcc attatcgagg ctggtgaagt ccaggcccgc
     1201 ttgctgcgcc tgctgcttgg cccactcgtg gccgttcaag cagacccgca ccgggtacgg
     1261 caggtaggtc ccgaccttca cgaaggccgg cccccactcc cggtcctgga cgtagaagta
     1321 gtagtggttg acggccaccg actgccgcga gaactcgaac agcacccccc gctcggaccc
     1381 ggtcagcttg cgcgctttga aggcactcgc ccgctcctgg gcgacgccga tgaacaccac
     1441 cccttcggcg ccgctgtatc gtgtccggtg ctcggcggcg atgtcgtcct tccgctgccc
     1501 acgctcgaag tgcacgaccg ggattttccc ggcgtcggcg aacgcgctca cctcacggac
     1561 gaaccgatcg tgcagcggac gcagcagggc cggcgacggg atcgggttcc ccaggtgctc
     1621 ctgcaggaac cagcacaact gcccggaggt ttgcagcttc ggcacgtagc cgttcaggta
     1681 cagccggtcg atgcagtcca ccgtcaacgt cacgcgatca cggataatgg cctcgatgtt
     1741 gggggtcgcc atcggagcct ccctcacggg tgggtgaaca ctccgatggt gcctccccaa
     1801 catccttccc gtcaccctct gctctccggc cagtgacctc cctcgtcggt ttgaggcgaa
     1861 gcttcgctag taccctgtca gccgtcatct gacgagttga acagggcagc agattcgctg
     1921 cgcg
//
LOCUS       DATADN010001029         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_728889, whole genome shotgun sequence.
ACCESSION   DATADN010001029 DATADN010000000
VERSION     DATADN010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728889"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VHH53_13255"
     CDS             complement(<1..617)
                     /locus_tag="VHH53_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator"
                     /protein_id="HEX2291129.1"
                     /translation="MTTTDPTAVDGAQLTRAQRAALPLSADVAQALAEQHGVCIRPLA
                     MRRINTTTGRVDIVPVPCGSIREDQCRPCADKARRLRMVQCRQGWHLDTEPITQRARP
                     SEQHQELMAARADLVAAYAQCQAIGDEVSCEQIADSVAELDTELRALGVRGRFTPLES
                     SPKAVKRSTRRRQDAPDLPRRPIDKRTVGRVFAGRYRPSTFLTLTL"
     gene            complement(614..>1789)
                     /locus_tag="VHH53_13260"
     CDS             complement(614..>1789)
                     /locus_tag="VHH53_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEX2291130.1"
                     /translation="IKHDAMPIVVTIPVLGRTLRRGRQHTRAQLPKVLGVRSGASWDE
                     VRIHLVPGQKPEDFDEAARALASARTVARCQVRELTPNVVSIDFQRRNLLAEPVACPD
                     LDDLAGVTGGSMDLRRVWAGRTEYGQDWHLPLSGSASHTLTAGASGAGKNSVMWCPLV
                     SIAPAIRDGLVRVSGIGPKGMELAYGRGIFHRYAVTGTDALTVLDELVEAMQARKAIF
                     AGRVRVVPISPEHPLELLEFDEIGALTKYTDRKTRDAITERVALLTTQGRALGFTVRG
                     YVQEPTKDTVPVRELFPRRICLRVAAKSHVGMVLGDQAYERGAWANRISDAEAGVGYV
                     FGEGIREPLRIRAGWVPDETIKHLETFVTNSAEYDTAVLPFPTKPTDPNGSSRGGAA"
BASE COUNT          263 a          574 c          637 g          315 t
ORIGIN      
        1 agcgtgagcg tcaggaaggt ggagggccgg tagcggccgg cgaacacccg gcccaccgtg
       61 cgcttgtcga tgggtcggcg gggcaggtcc ggggcatcct ggcgacgccg ggtggagcgt
      121 ttcaccgctt tgggtgaaga ctccagcggg gtgaaccgtc cgcgcacccc cagtgcccgc
      181 agctcggtgt cgagttcggc gaccgagtct gcgatctgtt cacaggacac ctcgtcgccg
      241 atggcctggc actgggcgta ggcagcgacc aagtcagccc gggcggccat caactcctga
      301 tgttgttcgc taggccgggc ccgttgggtg atcggttcgg tgtctaggtg ccagccctgg
      361 cggcattgga ccatccgcaa ccggcgggcc ttatccgcgc aggggcggca ctggtcttcc
      421 cgaatggacc cgcaggggac cgggacgatg tcaacgcggc cggtggtggt gttgatccgg
      481 cgcatggcca gcggccggat gcacacgccg tgctgctcgg ccagcgcctg cgccacatca
      541 gcactcagtg gcaaggcggc gcgctgtgcc cgggtcaact gcgcaccgtc tacagccgtg
      601 gggtcggtgg tggtcacgct gcgcctcccc ggcttgagcc gttggggtcg gtgggtttgg
      661 tggggaaggg cagtaccgcg gtgtcgtatt cggcggagtt cgtgacgaac gtttcgaggt
      721 gtttgatcgt ctcgtcgggg acccagccgg cgcggatgcg cagtggttct cggatgcctt
      781 cgccgaagac gtagccgacg ccggcttcgg cgtcgctgat gcggttggcc caggcgcccc
      841 gttcgtaggc ttgatcgccc agcaccatgc ccacgtggga cttggctgcc acccgcaggc
      901 agatccgtcg gggaaatagt tctcggactg ggacggtgtc tttggtgggt tcttggacgt
      961 agccgcgcac tgtgaacccc aacgcccgcc cctgcgtggt caaaagggcg accctttcgg
     1021 tgatggcgtc gcgggtctta cggtcggtgt atttggtgag cgctccgatc tcgtcgaatt
     1081 ccagcagctc cagtgggtgc tcagggctga tgggcacgac ccggacgcgg ccggcgaaga
     1141 tggctttgcg ggcctgcatc gcctcgacca gctcatcgag caccgtcagg gcgtcggtgc
     1201 cggtgaccgc gtagcggtga aagatgcctc gcccgtaggc aagttccatg cctttggggc
     1261 cgattccgga cactcgtacc aggccgtcgc ggatggcggg agcgatggag accagtgggc
     1321 accacatcac cgagttcttg cccgctccgc tggcaccggc ggtcagggtg tgactggccg
     1381 accccgacaa gggcaagtgc cagtcttggc cgtactcggt gcgcccggcc cacacccgcc
     1441 gcagatccat cgacccaccc gtgacacccg ccaggtcgtc caagtcgggg caggccaccg
     1501 gctcggcgag caggttgcgg cgctgaaaat caatcgacac cacattcggg gtgagttcgc
     1561 ggacctggca acgcgccacc gtccgggccg aggccaatgc tcgcgcggcc tcatcgaagt
     1621 cctccggttt ctggccgggc accaagtgga tccggacttc gtcccacgac gcaccggagc
     1681 gcacgccgag caccttgggc agctgcgccc gcgtgtgctg tcgtccccgc cgcagagtgc
     1741 gccccaggac agggatcgtc acgacgatgg gcatggcgtc gtgtttgat
//
LOCUS       DATADN010001030         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_868903, whole genome shotgun sequence.
ACCESSION   DATADN010001030 DATADN010000000
VERSION     DATADN010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868903"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VHH53_13265"
     CDS             <1..592
                     /locus_tag="VHH53_13265"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2291131.1"
                     /translation="AGLHEEHVTAHFGREVATVRVGRDSMVVTLDDGQRLEADATLAA
                     YGTIPASGWATGVDEGIAVDDRLRAANLPGVYAAGSVAVHTARNRHRYRVDHWDAATA
                     QGSHAARTLLHDLGRGEDPGPYVPSTGFTLILYRHAIAAYGVALPGAIQRQHLTGNPD
                     AILTTFHDPADQAQTAAVGLAAGRELLALRRQLQRP"
     gene            complement(650..2224)
                     /locus_tag="VHH53_13270"
     CDS             complement(650..2224)
                     /locus_tag="VHH53_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2291132.1"
                     /translation="MDVSLGGFVRALFQSWVSTGGVGLLLLSLSGCGFGGGDSGSEPS
                     ASDQRIVVANFRAPAAGWGLESDSAYILSLSGCLETLTRYVPARGEIVPMLATEWRQV
                     SPREWDFTLRKGVRFQDGSPMTAEAVVRSLQQVLAAEVPSRAFNRAVVSGVKAVDERT
                     VRVTTPTESPLVPLRMASVNTGVLAPAAFEGEGGVNPFGHCTGPFTPVSEQPQQSLTL
                     DRNEDYWGGQVQLAGAEVRFVTDGAARATQVQTGEADISLSVPVSELSTLEGDPGISV
                     LKSESPRTATLYMNNGRAPFDDIDFRRAVRSALDLDGLAASVYEGAAPPATGPFAPSE
                     PWAPDVERQRRDVEAAKGLLADAGYTADRPLEIIAIVERAELADVATVIQENLKDVGV
                     PVTITIKEYAAVEPDLLAGDYDMVLSQRNRLIDIADPIGFLTADYTCDGAYNLSHFCD
                     EKYDKLIVRAARTGDVEERYRLYGKAGRLLEEQAVNVWLVNEQAIDVVRSDVGSYVQD
                     PLARYVLTADTTKATS"
BASE COUNT          405 a          866 c          793 g          382 t
ORIGIN      
        1 cgcggggctg catgaagagc acgtgactgc tcacttcgga cgtgaggtcg ccactgtccg
       61 ggtcggccgg gactcgatgg tggtcacgct cgacgatgga caacggctgg aggccgacgc
      121 caccctggct gcctacggca ccattcccgc ctctggctgg gccacgggag tcgacgaggg
      181 catcgccgtt gacgatcggc tcagagccgc aaacctcccc ggtgtctacg ccgcggggag
      241 cgtggctgtg cataccgccc gcaaccggca ccgctaccgc gtcgaccact gggacgccgc
      301 cacggcccag ggctcacacg ccgcacggac tctgctccac gatctcggcc gcggtgagga
      361 ccctgggccc tacgtgccct cgaccgggtt cacactgatt ttgtaccggc atgcgatcgc
      421 cgcgtacggc gtcgcgctcc ccggcgccat ccagcgccag cacctcaccg ggaacccgga
      481 cgccatcctc accaccttcc acgaccccgc tgaccaggcg cagaccgcgg cggtggggct
      541 ggccgcgggg cgggagctcc tggcattgcg caggcagctg caacggccgt agcgtcgcgc
      601 cacttcgacg tgactgccgc gatcccacgg gattgtccgc gtctcggcat cacgacgtgg
      661 ccttggtggt gtccgcggtc agcacgtaac gagccagcgg gtcctggaca taggacccca
      721 cgtccgaccg gaccacgtcg atagcctgct cgttgacgag ccacacgttc accgcctgct
      781 cctcaaggag cctgccggcc ttgccgtaca ggcggtaccg ctcctcaaca tctccggtcc
      841 gagccgcccg gacgatcagc ttgtcgtact tctcgtcgca gaagtggctg aggttgtacg
      901 cgccgtcaca ggtgtagtcg gcggtgagga acccgatcgg atcggcgatg tcgatcaggc
      961 ggttgcgctg gctcaacacc atgtcgtagt ccccggccaa cagatccggc tccaccgccg
     1021 cgtactcctt gatcgtgatg gtcaccggga cgccaacgtc cttgaggttc tcctggatga
     1081 cagtggccac atccgcgagc tccgcacgtt ccacaatggc gatgatctcc agcggccgat
     1141 cggcggtgta ccccgcatca gcaagcagac ccttggctgc ctccacgtcc cgccgctgcc
     1201 tctcaacgtc cggggcccaa ggctcggagg gcgcgaaggg tcctgtggcc ggcggcgccg
     1261 caccctcgta cacgctggcc gctaggccgt ccaggtccag tgcggaacgg accgctctgc
     1321 ggaagtcgat gtcgtcgaac ggagccctgc cattgttcat gtacagggtg gcggtgcgag
     1381 gggactccga cttcagcacc gagatacccg ggtctccctc gagggtcgac agctcagaga
     1441 ccggcaccga cagggagata tccgcctcac cggtctgcac ctgggtggca cgagctgctc
     1501 cgtcggtgac gaaccgcacc tcggctccag ccagctgcac ctgaccgccc cagtagtcct
     1561 cgttgcgatc cagggtcagg gactgctgcg gttgctcaga taccggtgtg aacggaccgg
     1621 tgcagtgccc gaagggattc acgcctcctt ccccttcgaa ggcggccggg gcgagcacgc
     1681 cggtgttgac actggccatc cggaggggga ccagcgggct ctcggtcggt gtggtgacgc
     1741 gcaccgtacg ctcatccact gccttgacgc cgctgaccac ggcgcggttg aaggcccgcg
     1801 acgggacctc cgccgccaac acctgctgga gtgacctcac gaccgcctcc gccgtcatcg
     1861 ggctgccgtc ctggaaccga actcccttac gcaaagtgaa gtcccattcg cgcggcgaga
     1921 cctgacgcca ctcggtcgcc agcatgggca cgatctcccc tcgggcaggg acgtacctgg
     1981 tgagggtctc caggcacccg gacagggaga ggatgtaggc cgagtcgctc tccaggcccc
     2041 agccagcggc cggggctcgg aagttggcca ccacgatccg ctggtcggag gcagacggct
     2101 cggatccact gtccccgccg ccgaagccgc agcccgagag cgacagaagg agtaggccga
     2161 ccccgccggt ggagacccac gactggaaca acgcgcgcac gaaaccgcct agagaaacgt
     2221 ccacagtgga ctgaagagtt gagggcactg aggggcacta agggtagcct cacctaaatg
     2281 gcccgctcct catggggtcg gggttccgac ctcatcccct ggaccacgac gtcctcaccg
     2341 cggacctcgc cgtcgatcgc tgagggagga gccgctgggt gaacgttccg cgtcgggatt
     2401 ccgccgcggc ctgcttcctg ggtagcgtcg gccggcgaag cggcac
//
LOCUS       DATADN010001031         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1039276, whole genome shotgun sequence.
ACCESSION   DATADN010001031 DATADN010000000
VERSION     DATADN010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039276"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VHH53_13275"
     CDS             <1..596
                     /locus_tag="VHH53_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2291133.1"
                     /translation="QGQCGHQGATLGIPSSPSAAIILAALVLQGARPGPSLMTDQSEV
                     MFTFFAALLVTNILMIPVGMAILRLCMTVITVSAPVLTSAVLVLSLAGTYALNQNIVD
                     VQVAVLFGFIGYAMKVYGFSPAAAVLGLVLGYIMEGELRRSMMMSLGEPTIFLTRPIS
                     ALILLLAVLVLARPAIAWAIRSVRGTRPHHEKGVAQK"
     gene            679..>1580
                     /locus_tag="VHH53_13280"
     CDS             679..>1580
                     /locus_tag="VHH53_13280"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX2291134.1"
                     /translation="MAAIRFRHRAARSAAVLLALVTLAACQIAAGGAGGGGAEEEFPT
                     KPISMVVGFAPGGGADVFARALTRAAQQTLPEQVVVENREGGSGTSASAFVHGQPADG
                     YTILFGHAGSTILTPTISNSPQLKWDAFAPVARIHAEEEFLFLRPDAAWDTIEELVQY
                     ARDNPGAVRVGGSAVGGVDSFVVFQLEKAAGVDFTYVPFDGGGPASMSFLGGNVDVLI
                     GNLSDNVSSVDSGEMRPIAVASAQRGPQTDVPTLAENGWDVVLQQWRGVFAPKGTPPE
                     RIKILADGFEAALGEQAWVTYRESS"
BASE COUNT          252 a          498 c          540 g          290 t
ORIGIN      
        1 cccagggtca gtgcgggcac cagggcgcca ccctgggaat cccgagctcg ccatcggcgg
       61 cgatcattct ggcggcgctg gtcctgcagg gtgctcgacc cgggccgtcg ctgatgaccg
      121 accagtccga ggtgatgttc accttcttcg ccgcgctact ggtgaccaac atcctgatga
      181 ttccggtcgg catggcgatc ctgcgcctgt gcatgaccgt gatcacggtc agcgcgccgg
      241 tgctgaccag cgcggtgctg gtgctttcgc tggccgggac gtatgcgttg aaccagaaca
      301 tcgtcgacgt gcaagtcgcg gtgctcttcg gtttcatcgg gtatgcgatg aaggtctacg
      361 ggttctcccc cgcggccgcc gtgctcggtc tggtgctcgg ctacatcatg gaaggggagc
      421 tccgacgttc catgatgatg tcgctgggtg agccgacgat cttccttacc cgaccgatct
      481 ccgcactgat cctgttgttg gcggtgcttg tgttggcacg cccggcgatc gcgtgggcga
      541 tccggtccgt tcggggaaca cgacctcacc acgagaaggg agtcgcccag aagtagccag
      601 cgcctctcgt tcgaccaggc gctggtctgc gctacacagg tgggcaacgg tgcccgccac
      661 ggagaaggga agattgccat ggcagccatt cgcttccgac accgcgcagc acggtcggcc
      721 gccgttttgc tcgctcttgt gacgctcgcc gcatgccaga tcgcggccgg cggcgcgggc
      781 gggggcgggg ccgaggagga gttcccgacc aagcccatca gcatggtcgt cgggtttgcg
      841 cccggtggtg gtgcggatgt attcgcacga gcactcacca gggcggcaca gcagacgctc
      901 ccggagcagg ttgtggtgga gaaccgggag ggaggcagcg gcaccagcgc cagcgctttc
      961 gtgcacggcc agcccgctga cggctacacc atcctcttcg gacacgccgg atccacgatt
     1021 ctcaccccga cgatcagcaa cagcccgcag ctgaaatggg acgccttcgc cccggtcgcg
     1081 cgaatccacg ccgaggagga gttcctgttc ctacgtcccg acgcagcctg ggacacgatc
     1141 gaagaactgg tgcagtacgc gcgggacaat cccggcgccg ttcgcgtggg cggttccgct
     1201 gttggcggag tggacagttt cgtcgtcttc cagttggaaa aggctgctgg tgtcgacttc
     1261 acctacgtgc ccttcgacgg tggtgggccg gcgtccatga gtttcctcgg tggcaacgtg
     1321 gacgtcctga tcggaaacct gtccgacaac gtctccagcg tcgactcggg tgagatgcgc
     1381 ccgatcgcgg tcgccagtgc gcagcgggga ccgcagaccg acgtgccgac cctcgccgag
     1441 aacggctggg acgtggtgtt gcagcagtgg cgtggtgtct tcgcgcccaa ggggacacct
     1501 ccggaacgaa tcaagatcct ggcggacggg ttcgaagcgg cgttgggtga gcaggcatgg
     1561 gtgacctacc gcgaatcgtc
//
LOCUS       DATADN010001032         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_268072, whole genome shotgun sequence.
ACCESSION   DATADN010001032 DATADN010000000
VERSION     DATADN010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268072"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1272)
                     /locus_tag="VHH53_13285"
                     /pseudo
     CDS             complement(1..1272)
                     /locus_tag="VHH53_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443330.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccC"
     gene            1540..>1777
                     /locus_tag="VHH53_13290"
     CDS             1540..>1777
                     /locus_tag="VHH53_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEX2291135.1"
                     /translation="MTNGVSAVRPSVAGLTVAPSTVFSRVTVVAPRTRIDLALASDVA
                     VANLLPMVLEMAGEAAPDGGSRHGGWCLAKLSGDA"
BASE COUNT          293 a          627 c          576 g          281 t
ORIGIN      
        1 gcagctccag caggcccgga ttggccagca gggctcgtcg ctgctgtcgg cttcctccgg
       61 accgatccgg tacggggaca gcgtgcgcgc caccgcctcg gcctcagcga caccgagcga
      121 atcgggaacc ccgaagaatt cgactccggc cggcccgcgg gcgccgatcg ccgcgccctc
      181 gatcgtcagc tgcagcccgc ggcgggtggt caggctgcca agcgagtccg agaggtcgag
      241 cagggtgacc ccggccatgc cctcctccaa gaggatctgc tcctccccag tgacctctcc
      301 accgtcgatg atgatcacta ggtgcgggcc gtcaccgtag gtctgggcgt tgcgggagaa
      361 ccgagggcgg tcggccagct gctcgccgag caacctctct atctgcgtca atgatccggc
      421 catcatccgc ctcggcccgg cgccatcgac cagactcggg tgctgcacgt gtggcagcca
      481 cttcgtccat tcccagtccg cctttgcccg cccggtggtg accacggcaa tgagcaggtc
      541 atcgggaggg tggaaggtga ccagctgggc caccatcgag cgggccagct cgcgcgtcga
      601 gacgtgcgcg ccggaaatcc cgattgccgc gaatcctcga accgacagtg agatcggcag
      661 atcgggcacg atggaatggg cccggacgaa ctggcgcaac gccagcgtgg tgatcggttc
      721 cagctcgtcc accggaccgg tctgtggcgc caccagccgg gtggccagcc gctgcgagcc
      781 tcggcagatc cgtacgtgac agaagtccac gtcgccgctg cgacgctccc acatccgccg
      841 gctgctcgcg atcgaccaca gcgcggcggg atcgggatgg gtccactcca acgccgcgcg
      901 ctgttccagc gccgcctttc tggcgcgttc ccgcatctgg cccaggtagc gcaggtagtc
      961 cttacggtcc tcattcatct cagccttgac ctggccacgc ccgcggccgc caccaacgaa
     1021 catgccgacg gtcgacacca gcatcatgat cggaaacagc aggaacaccg ggttgctctt
     1081 ggcgtccccg gtggtgaaga cgaacgccac catgcccaga acagccgcga tcatcacgac
     1141 cggcaggatc ttctgcacga tgttgcccgg caggaccctg ggcacctcgg gtggtgcttc
     1201 gaggtgtacc tcaccaccgg gcatcttcgg cgcggccagt cgaggtgacc gccggaactg
     1261 caccgtgctc acgccagctg acctccgcac cctcgtgggc gaggcgacac cctagcgggt
     1321 tcctggagct gccggccgtc gagcacttcg ccctcatcga tccgctctca gccgcctggt
     1381 cacacgtcct cgctgctctc gcccgggata gacccccttg agcggttagg cacggtccgc
     1441 atggatactc caccgcacca agaaggccgc cgtgaccccg caccgatgtg cataccaacg
     1501 gtcggcaggc cgagcgagag ggtgcagcgg ttgccaacca taaccaatgg agtttccgcg
     1561 gtgcggccgt cggttgctgg gctgacggtt gcgccgtcca ccgtgttcag cagggtgaca
     1621 gtggtggcac cgcggacacg catcgacctg gcgctagcgt cggacgtcgc ggtggcgaac
     1681 ctgcttccca tggtgcttga gatggccggt gaagctgctc ccgacggggg cagcaggcac
     1741 ggcggctggt gcctggccaa actcagcggt gacgcga
//
LOCUS       DATADN010001033         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_869392, whole genome shotgun sequence.
ACCESSION   DATADN010001033 DATADN010000000
VERSION     DATADN010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869392"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            615..1664
                     /locus_tag="VHH53_13295"
     CDS             615..1664
                     /locus_tag="VHH53_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX2291136.1"
                     /translation="MSFVGKMRDAVKAMPRRLTMGAMAVVTATAVVPGLVGVAGGSAT
                     AGAFSRPGLPVEYLMVPSAGMGRDIKVQFQSGGANAPGVYLLDGLRAQEDFSGWDINT
                     AAFEWYLDSGLSVIMPVGGQSSFYSDWYKPACGKAGCSTYKWETFLTSELPAYLAANK
                     GVNPNRNAAVGLSMAGSAAMTLAIYYPQQFQYAASLSGFLNLSEGWWPMLVGLSMGDA
                     GGYKSEDMWGPSSDPAWKRNDPMVNIDKLVANGTRVWVFCGNGKPADINGSGVAAGDN
                     FNAKFLESFTLRTNETFQEQYLAAGGRNGVFNFPQSGTHSWEYWGQQLQQMKPNIQRV
                     LGAVPQPSPAPAPAG"
BASE COUNT          278 a          559 c          580 g          268 t
ORIGIN      
        1 accgcttgga ccgggtgccg cgctacgagc tgatccggtg cggactgccc atccccgcac
       61 cccatgagcc gccctacacg ggtatgccgg cggccggacc gtgacggacc gctggtggcg
      121 aacgccgatt tgtgggccgg gcctcccgtc tgtttgggta acgccggggg caggtcgaag
      181 cgatatcccg ttcaggtgtc ccgaacaccg gttgaacgct gccctgacga ctgccgtggc
      241 aacggcgccg aacccgatcg aaggaccgca gatgcgtagc acgtcgcgta tcgacccggc
      301 cgcgcgtgcg cgcgtgcccg ccggggtgaa cgcggtccgc cggccccggg tgatcaattc
      361 cacgcgccac ctcctcggcc acgttgccac cctcggatcc cacctcggcg ggcacccgcg
      421 cgccggggct ctgaggtgcg cggatgcacc tttcaccacc cggctcgacc gccgcctgca
      481 acgatgctgc agcggtcgtg gcgaaacacg ctggagagaa cgatcacaca atcgtgtggt
      541 tgactacacg ggcaacgcgg ctggtctgga gtgcccggac cggttgcggc ttacgacaga
      601 tgggaaatag cagtatgagt ttcgttggga agatgcgcga cgcggtgaaa gccatgccgc
      661 gccggctcac gatgggcgcc atggccgtcg tcacggccac cgccgttgtg ccgggcctgg
      721 tgggcgtcgc cggaggctcg gcgactgcag gggcgttctc gcgtcccggt ctgccggtcg
      781 agtacctcat ggtcccgtct gcgggcatgg gccgcgacat caaggtccag ttccagagcg
      841 gcggcgccaa tgcaccgggt gtctacctgc tcgacggcct gcgtgcgcag gaagacttca
      901 gcggctggga catcaacacc gccgcattcg agtggtacct ggactccggc ctctcggtga
      961 tcatgccggt cggcggccag tccagcttct acagcgactg gtacaagccg gcctgcggta
     1021 aggccggttg ctccacctac aagtgggaga cattcctgac cagcgagctg ccggcctacc
     1081 tggcggccaa caagggtgtg aacccgaacc gcaacgccgc ggtgggcctg tcgatggccg
     1141 gttcggccgc gatgacgctg gcgatctact acccgcagca gttccagtac gcggcgtcgc
     1201 tgtcgggctt cctgaacctg tccgagggct ggtggccgat gctggtgggc ctgtccatgg
     1261 gtgacgccgg cggctacaag tccgaggaca tgtggggccc ctcgagcgat cccgcgtgga
     1321 agcgcaacga cccgatggtc aacatcgaca agctggtcgc caacggcacc cgcgtctggg
     1381 tgttctgcgg taacggcaag ccggccgaca tcaacggcag cggcgtcgcc gcgggtgaca
     1441 acttcaacgc gaagttcctg gagagcttca cgctgcggac gaacgagacg ttccaggagc
     1501 agtacctggc cgcgggtggg cgcaacggcg tcttcaactt cccgcagtcc ggtacgcaca
     1561 gctgggagta ctggggtcag cagctgcagc agatgaagcc gaacatccag cgcgtgctgg
     1621 gtgcggtgcc gcagccgagc cccgcgccgg cgccggctgg ttgaaccgac agctgacgta
     1681 acgat
//
LOCUS       DATADN010001034         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_869508, whole genome shotgun sequence.
ACCESSION   DATADN010001034 DATADN010000000
VERSION     DATADN010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869508"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VHH53_13300"
     CDS             <1..584
                     /locus_tag="VHH53_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2291137.1"
                     /translation="AFAAAVPTARVLVNNAGGAKGLDPVLEADEAQWRWMWEANVLGT
                     LRMTKALLPKLIDSGDGVLVTVTSIAALEAYDNGSGYTSAKHAQAAAHRTLRGEHLGQ
                     PVRFIEIAPGMVETEFSVVRFGGDKQRADGVYAGLTPLSAHDVADVIAFAVSRPAHVN
                     LDLIVVKPRAQHSAMRSARGLDTDCDSVDARPR"
     gene            595..1086
                     /locus_tag="VHH53_13305"
     CDS             595..1086
                     /locus_tag="VHH53_13305"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HEX2291138.1"
                     /translation="MASRLQLRHRYPEPPERIQAMLTDPEYLRGKLRAVGGPGAELVS
                     REQDGRGVTVVLRQAVPDDALPSFLRSAFSGGLTIRRTETWTNSGGSVHAVVDGAPGT
                     ITGTMRLEPEPDGCILGAELTAEVPLPLIGGKVERVITDNIGKLMDAEHQFTLQWLRD
                     TGA"
     gene            1098..>1745
                     /locus_tag="VHH53_13310"
     CDS             1098..>1745
                     /locus_tag="VHH53_13310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX2291139.1"
                     /translation="MELDEYQRRIAVEAGLLRAAAVSVDAGTLVPTCPGWTTQRLVQH
                     VGRVFDMVIRVLKAADPGSPPVRVPPPSGDVFAGFDDRLAILLDLLAATDPATPVWHF
                     SPTAPKTAAFWSRRTAHEVTVHRIDAQSAAGIDSAVPPDFAADGVDEVLTRLIARHTD
                     KWAVAELSGTVLYHAADAGRAWAVRLVPGQLPQTVPEAVTEPDASVIGLADAVYRA"
BASE COUNT          259 a          604 c          607 g          275 t
ORIGIN      
        1 ccgccttcgc cgcggcggta cccacagccc gggtgctggt caataacgcc ggcggcgcca
       61 aggggctcga tccggtcctg gaggccgacg aggcccagtg gcgctggatg tgggaggcca
      121 acgtgcttgg gacgctacgg atgaccaagg ctttgctgcc gaagttgatc gactccgggg
      181 atggcgtgct ggtcacggtt acctcgatcg ccgctctgga agcctacgac aacggctcgg
      241 gttacacctc agccaaacac gcccaagcag cggcgcaccg cacattgcgc ggtgagcacc
      301 tcgggcagcc ggtacggttc atcgagatcg ctcccggcat ggtggagacc gagttctctg
      361 tggtgcgttt cggcggcgac aagcagcggg cggacggggt ctacgccggg ctgaccccgc
      421 tgagcgcgca cgacgtcgcc gacgtcatcg ccttcgccgt cagccgcccc gcacacgtca
      481 acctcgatct catcgtggtc aagccccgtg cccagcactc cgccatgcgt tccgcccgcg
      541 gcctggatac agactgcgac tcggtcgacg cgcgtccacg gtagcctccg cgccatggcc
      601 agccgattgc agctgcggca ccgttacccc gagccgccgg agcggatcca ggcgatgctg
      661 accgacccgg agtacctgcg tggcaagctg cgtgcggtag gaggtccggg ggccgagcta
      721 gtgtcgcggg agcaggacgg gcgcggcgtc accgtcgtgc tgcgccaagc tgtgcccgac
      781 gacgcgctgc cgtccttcct ccgttctgca ttttccggcg gtttgacgat ccgccgtacc
      841 gaaacctgga ccaactccgg cggcagcgtg cacgcagtgg tcgacggggc accggggacc
      901 atcaccggga cgatgcgtct ggagccggag cccgacggct gcatactggg tgccgagctc
      961 accgccgagg tcccgctgcc gctgatcggc ggcaaggtgg agagagtcat caccgacaac
     1021 atcggcaagc tgatggacgc cgagcaccag ttcacacttc agtggctgcg cgacaccggg
     1081 gcttaggctg gcccaccgtg gaactcgacg agtaccagcg caggatcgcc gtcgaagccg
     1141 gcctgttacg agctgcggcc gtgtccgtcg atgcgggcac cctggtgccg acctgcccgg
     1201 gttggaccac gcagcggttg gtgcagcacg tcggccgggt ctttgacatg gtcatcaggg
     1261 tgctcaaggc cgctgatccg gggtcgccgc cggtgcgggt accgccgccg tcgggcgacg
     1321 tgttcgccgg tttcgacgat cggttggcca tcctgctcga cctgctagcg gccaccgatc
     1381 ccgccacccc ggtctggcac ttctccccca cggcgccgaa gactgcggcg ttctggtccc
     1441 gccggacggc gcatgaggtc acggtgcacc ggatcgatgc gcagtcagcg gcgggcatcg
     1501 attcggcagt tcccccggat ttcgccgcgg acggcgtgga cgaagtgctg acccggctga
     1561 tcgcgcggca taccgataag tgggccgtcg cggagttgtc gggcacggtg ctctaccacg
     1621 ccgcggacgc cgggcgggcc tgggcggtcc gcctcgtacc gggtcagctg ccccagaccg
     1681 tgcccgaggc ggtgaccgag ccggacgcct cggtgatcgg gcttgccgac gccgtctacc
     1741 gcgcc
//
LOCUS       DATADN010001035         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_869518, whole genome shotgun sequence.
ACCESSION   DATADN010001035 DATADN010000000
VERSION     DATADN010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869518"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VHH53_13315"
     CDS             complement(<1..971)
                     /locus_tag="VHH53_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX2291140.1"
                     /translation="MIGVRIHWLCATMIGLALLVSNCTAAPGPSLPGGPVECTNGVIE
                     PGQASAALQQVRPGGIVCLSGDGLAGAELEVTTSGTPQQPITIIADGATMRSLNVNAN
                     YVVIQGLTLRDGDGLTMAGRGLVARNNVIYNATADGVECRDCADTVIESNTVERADGT
                     GIWIAGERITVHQNTVSDSVLRTQGDADGIRFFGNGHRLTRNTIKDIKASGYSRGDPH
                     TDCFQTYDSRDAPPTYDVIIANNVCTNVDVQALIATTDDRRGGGAPTGQIAIVFEHNT
                     VSVNGSQAILLRNFPRVIVRNNRFSGPGHRSVMLTEGSTDCAVIDNT"
     gene            1100..1516
                     /locus_tag="VHH53_13320"
     CDS             1100..1516
                     /locus_tag="VHH53_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291141.1"
                     /translation="MTQPTNESAGSVMGWTLNPTLTPDARALRAAQLLLWAQVSTGRH
                     DAGWQNTAAEAGLEVRAPRKRHDAYYIWTGQGSGSLTVGQMVRTVHGDVGGDLYSEWS
                     PASHTDPLSLAKLARIRHSESGFHVGGLIVRTTTSR"
BASE COUNT          284 a          482 c          509 g          277 t
ORIGIN      
        1 atggtgttgt caatcactgc gcagtcggtg gaaccctcag tgagcatcac cgagcggtgt
       61 ccgggtccgg agaagcggtt gttccgcacg atgacccggg ggaagttccg tagcaagata
      121 gcttgagaac cgttgaccga gacggtgttg tgctcgaaga cgatcgcgat ctgtcccgtc
      181 ggtgcgccac cgccacggcg gtcgtcggta gtggcgatca gggcctggac gtcgacgttg
      241 gtgcagacgt tattggcgat gatgacgtcg taagtaggtg gagcatcacg gctgtcatag
      301 gtctggaaac agtcggtgtg cggatccccg cgggagtagc cggacgcctt gatatccttg
      361 atcgtattcc tggtcaaccg gtgaccattc ccgaagaacc ggatgccatc ggcgtcgccc
      421 tgagtgcgca gcaccgagtc gctcaccgtg ttttggtgga cggtgatccg ctccccggca
      481 atccagatac cggtcccgtc ggcacgctca acggtgttcg actcgatgac cgtgtccgcg
      541 cagtcccggc attccacgcc gtccgcggtg gcgttgtaaa tcacattatt acgggcgacg
      601 agtccacgac ctgccatcgt caggccatcc ccatctcgca gtgtcaggcc ctggatgacg
      661 acgtagttag cgttgacgtt caggctgcgc atcgtcgcgc cgtcggcgat gatcgtgatc
      721 ggctgctgag gtgtaccgga ggttgtcacc tccagctcgg caccggccag accgtcgccg
      781 ctcaagcaca cgatgccgcc gggccggact tgctgcaggg cagcgctggc ctgaccgggt
      841 tcgatcaccc catttgtgca ctccaccgga ccgccgggta gcgaggggcc gggggcagca
      901 gtgcaattgc tgacaagcag cgccaagccg atcatcgtgg cgcacagcca gtggattcgg
      961 acaccgatca cttgcttacc ccgcgtgcat cagcgggtag ccagggatgt cgggccatgc
     1021 tggcgcgtcg gcgcacctcg agactgtagc ccaccgcgcg ctcactgaga tgcgcgcagc
     1081 gaggagatgc tgacagacag tgacgcagcc aaccaacgaa tcggccggct ccgtgatggg
     1141 ctggacgctc aacccgacgc tcaccccaga tgctcgcgcg ctccgcgcgg cccagctcct
     1201 gctgtgggca caagtgtcta ccggcaggca cgacgccgga tggcagaaca cggcggccga
     1261 ggcgggattg gaggttcgcg cgcccagaaa acgccatgac gcgtactaca tctggaccgg
     1321 ccagggctcc ggatcgctga ctgtgggcca gatggtcaga accgtccacg gtgacgtggg
     1381 aggtgacctc tacagcgagt ggagtccggc tagccacacc gacccgctat cgctggcgaa
     1441 gctggccagg atccgacata gcgagtccgg gtttcatgtc ggcggattga tcgtgaggac
     1501 cacgacgtcg aggtagccgt acacgttgcc gacctgatca ccgccgcgat ca
//
LOCUS       DATADN010001036         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_730111, whole genome shotgun sequence.
ACCESSION   DATADN010001036 DATADN010000000
VERSION     DATADN010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730111"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VHH53_13325"
     CDS             complement(<1..692)
                     /locus_tag="VHH53_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX2291142.1"
                     /translation="MTRWGLVLGALVKVLLVSTLSAALLSSSVSPPLLSHHSAEQPAW
                     STRSIPPPDALPDPLPDQLPALPLVSPFTGLVTDLSAPVLTVKIDNARSARPQTGLTL
                     ADVVYVEPVEGGISRLLAVYQSQFPPVVGPVRSTRLTDLQLLANFGRPALAFSGAAAE
                     IGALVAQAPVLDVSADEQPGSYRRDRTRRMPHNLYADTNLLRQGGAPPRDIGFRFGPT
                     PPGGRPMPETNVR"
     gene            1037..>2025
                     /locus_tag="VHH53_13330"
     CDS             1037..>2025
                     /locus_tag="VHH53_13330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2291143.1"
                     /translation="MSHGFDHVVFRRGLAALHPRQWKLWAERRAAVAYILTVDVVAVA
                     LLAGVIVAIPVRSEDLFRFCLLVAGAVLHQEAVRQIEIQRERTVGSGLLPNLKSLWIF
                     AAALSVPLPLVFAVTMIVYLHSWYRISPGRLYRKLFSAATMVLAPAAAVAVLQLVAAN
                     EYPIIPDGLPGLAALIGAGAVYWLVNFGLVAAAVMMSDPLQPTRQALGNPNDQVVVAA
                     SLGLGVALAAMLAWQPWLIAVLMITVIALHRALLLPHFQQAARTDSKTGLLTVGFWHE
                     VAGREIERARRLAEPLGVLMLDLDHFKSFNDRMGHLVGDQLLRAVADELRAETR"
BASE COUNT          329 a          636 c          689 g          371 t
ORIGIN      
        1 cggacgttgg tctccggcat cgggcggcca ccgggcgggg tgggaccgaa gcggaacccg
       61 atgtcgcgcg gcggtgcacc gccctggcgt agcaggttgg tgtcggcgta gagattgtgc
      121 ggcatccgtc ttgtccgatc ccggcggtag ctgcccggct gttcatcagc cgagacgtcg
      181 agcaccggcg cttgcgccac cagtgcaccg atctcggcgg cagcgccaga gaacgccaac
      241 gccggtcggc cgaaattggc cagcagttgc aggtcggtca gccgcgtgct gcgcaccggc
      301 cccaccaccg gtgggaattg agactggtac accgcgagca gccggctgat gcccccctca
      361 accggctcga catataccac atcggcaagg gtgaggccgg tctgcggacg cgccgaccgg
      421 gcattgtcga tcttgacggt gagcacgggc gcgctgaggt cggtcaccag accggtgaac
      481 ggcgatacca gcggcagtgc gggtagctga tcgggcagtg gatccggtaa ggcgtcaggc
      541 ggtgggatgc tccgggtgga ccaagcaggt tgctcggccg agtggtgtga cagcagcggt
      601 ggggaaaccg aggaggacag cagcgcagcg gaaagcgtgg agacgagcaa gaccttaacc
      661 agcgcgccca gcacgagccc ccaccgcgtc atgaccaccc ccctctcgcc ggtgcaggat
      721 gcaacgctga gtgacacatt tcatacgtag cgtaatggga gcaggtgacg ccacggagtc
      781 ccgctcttct attatctgca ccgttgctcg ttgccggcta tgcgattgcc catgtggcta
      841 caacaggtcc acgccgcgcg ctggccgacg accatgatga cgtgaccttc cgaagttctt
      901 gccgtaaacc attgattaca gaaagtaatt gaagctcgcc tgaatgtggc acttcgctcg
      961 gaggatgaca aagagtcgca ggccgattac cctgggggag attgctcaac gtcaccaccg
     1021 ggggccccta gtgagcgtga gccacgggtt tgatcacgtc gtgttccggc gtggcctggc
     1081 ggctctgcac ccgcggcagt ggaagctttg ggccgagcgg cgggccgcgg tcgcctacat
     1141 cctcaccgtg gacgtggtgg cagtggcctt gctcgccggc gtgatcgtgg cgatcccggt
     1201 ccggtcggag gatctgttcc ggttctgcct gctggttgcg ggggcggttc tccaccagga
     1261 ggcggttcgc cagatcgaga tccagcggga gcggacagtc ggcagcggcc tgctcccgaa
     1321 cctgaaatca ttgtggatct tcgcggcggc gttgtcggtc ccgctaccgc tggtcttcgc
     1381 cgtcaccatg attgtgtacc tccactcctg gtaccggatc agccctggcc gcctctaccg
     1441 caagctgttt tcggctgcga cgatggtgct ggcgccggct gctgccgtcg cggtcctgca
     1501 gctggtggcg gccaacgagt acccgatcat tccagatggt ctgccgggac tggcggccct
     1561 gatcggcgcc ggagcggtgt actggctggt caatttcggg ctggtcgccg ctgccgtgat
     1621 gatgtccgat ccgttgcagc caacccgtca ggcattaggc aacccgaacg atcaggttgt
     1681 tgttgcggcg tcgctcggac tgggcgtggc gcttgccgcc atgctggcct ggcagccgtg
     1741 gctgatcgcc gttctcatga tcaccgtgat cgcgctgcat cgcgcattgc tgttgccgca
     1801 cttccagcag gccgcccgca ccgacagcaa gacaggtttg ctcaccgtgg gcttctggca
     1861 cgaggtcgcc ggccgggaaa tcgaacgggc ccgccggctc gccgagccgc tcggagtgtt
     1921 gatgctcgac ctggaccact tcaaatcgtt caacgaccgg atggggcatc tcgtcggcga
     1981 tcaactgctg cgcgccgtcg ctgacgaact gcgcgctgag acccg
//
LOCUS       DATADN010001037         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1190682, whole genome shotgun sequence.
ACCESSION   DATADN010001037 DATADN010000000
VERSION     DATADN010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190682"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..3120)
                     /gene="gcvP"
                     /locus_tag="VHH53_13335"
     CDS             complement(205..3120)
                     /gene="gcvP"
                     /locus_tag="VHH53_13335"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX2291144.1"
                     /translation="MNPSDRRPLSELEHGTPFADRHIGPRPGELARMLATIGVESLDE
                     LAQQALPPTVVDPYAASNLPAPATEEQALTELRARAARCHPRIPMIGMGYHGTVTPPV
                     IRRSVLENPAWYTAYTPYQPEISQGRLEALLNFQTVVADLTGLPVANASLLDEATAAA
                     EAMTLLHRAGRTSSSRFVVDADTLPQTLAVLATRAEPLGIELDVADVFGKGSGPSLPD
                     GEFFGVLLSYPGASGAVRDHAMLIEQAHQRGAAVAVAADPLALTLLRPPGEIGADVAV
                     GSTQRFGVPIGFGGPHAAYLAVRHGLERQMPGRLVGMSRDADGNPAYRLALQTREQHI
                     RRERATSNICTAQVLLAVMASMYAIYHGPDGLRDIARRAHRMAAVLAAGLRAGGVEVV
                     SEEFFDTVLARVPGRAAEVVAAARRAGVDLRLAGADHVGISCAETTTRGHLQLVWDAF
                     GVRGADPDVLDRQTPDAIPAALQRTSPFLTHPVFSAYHSETALLRYLRALADKDVALD
                     RSMIPLGSCTMKLNATAELEPISWPQFAELHPFAALADAQGLLEVVSDLELWLAELTG
                     YDAVSLQPNAGSQGELAGLLAIRAYHRSRGETGRDVCLIPSSAHGTNAASAVMAGMRV
                     VVVGCDRNGDVDLDDLRGKIAKHGAELAAIMITYPSTHGVYEPAVTDVCALVHDAGGQ
                     VYVDGANLNALLGLARPGRFGADVSHLNLHKTFCIPHGGGGPGVGPIGVRAHLAPFLP
                     GHPQTVPDAVVGPVAAAPWGSASILPISWAYIRMMGAQGLRRATLTAVAAANYVARRL
                     GPHFPVLYAGNPANGYGSTTQGLVAHECILDLRPITKATGVTVDDVAKRLADYGLHAP
                     TMSFPVAGTLMVEPTESENLAELDRFCDAMIAIRDEIDRLAEGEWPADDNPLRCAPHT
                     ASCLADKWDHPYSRELAAYPMGRTAAKVWPPVRRVHQAHGDRNLMCVCPPLAESTAQ"
     regulatory      complement(3173..3298)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
BASE COUNT          529 a         1135 c         1147 g          516 t
ORIGIN      
        1 aaccgcgcgt cctccaggtt cgaccgggac gctgcaaacc tacaggtgca cgagctccag
       61 ggcggcgcgg aagtcgtcga cttctaaaac aaatcattcc cacgaggacc tacgatggcc
      121 ggattgaggg cattttcgaa gacgcagggc catgcacaag ggaattcaac ccggtgatcg
      181 tggccgaatc actgagcttc tcaatcactg agccgtgctc tcggcgagtg gggggcatac
      241 gcacatcagg ttgcggtccc cgtgtgcctg gtggacgcgc cgcaccggcg gccacacctt
      301 ggcggccgtt cgacccatcg ggtacgcggc gagctcccgg ctgtaggggt gatcccactt
      361 gtcggccagg caactggcgg tgtgcggagc gcaacgcaac ggattgtcgt cagcaggcca
      421 ctccccctcg gcgagccggt cgatctcgtc ccggatcgcg atcatcgcgt cgcagaaacg
      481 atccagctca gcaaggttct cgctttcggt gggctcgacc atcagggtcc cggccaccgg
      541 gaaggacatg gtgggggcgt gcagcccgta gtcggccagc cgcttggcga catcgtccac
      601 ggtgacgccg gtggctttgg tgatgggtcg caggtccagg atgcactcgt gggcaaccag
      661 cccttgcgtg gtcgagccat agccattggc ggggttgccg gcgtagagta ccgggaagtg
      721 cggcccgagg cgccgggcta cgtaattcgc ggctgccact gcggtgagcg tggcgcggcg
      781 caggccctga gcacccatca tccggatgta ggcccaggag atcggcagga tcgacgcgct
      841 gccccacggc gctgccgcca ccggacccac cacagcgtcg ggaaccgtct gtgggtgtcc
      901 gggtaggaaa ggcgccaggt gcgcccgtac tccgatcgga cccactccgg gcccgccgcc
      961 gccgtgcggg atacagaacg tcttgtgcag gttgaggtgg ctgacgtcgg cgccgaagcg
     1021 acccggccgg gccaatccga gcagcgcgtt gaggttggcc ccgtcgacgt agacctgccc
     1081 cccggcgtcg tgcaccagcg cgcacacgtc ggtcacagcc ggctcgtaca ccccgtgtgt
     1141 ggacggatag gtgatcatga tggcggccag ttcagcaccg tgcttggcga tcttcccgcg
     1201 gaggtcgtcg aggtcgacgt cgccattgcg gtcgcatccg accaccacga cgcgcatccc
     1261 ggccatcacc gccgacgcag cgttggtgcc gtgcgcgctg gacgggatca ggcagacgtc
     1321 gcggcccgtc tcgccacggc tgcggtgata ggcgcggatc gccagcaggc cggcgagctc
     1381 tccctggctg ccggcgttgg gctgcaggct gaccgcgtca taaccggtga gctcggccaa
     1441 ccaaagctcc aggtcgctga ccacttcgag taggccctgc gcgtcggcca gagcagcaaa
     1501 cgggtgcagt tcggcgaact gcggccagct gatcggctcc agctcggcgg tggcgttgag
     1561 cttcatggtg cacgagccca gcgggatcat gctgcgatcc agcgccacgt ccttgtcggc
     1621 cagcgcgcgc aggtaacgca gcagcgccgt ctcgctgtgg tacgccgaga agaccgggtg
     1681 agtcaggaac ggcgaggtcc gctgcaacgc cgcgggaatg gcgtccggcg tctggcggtc
     1741 aaggacgtcc ggatcggcgc cacgcacccc gaaggcatcc cagaccagct gcaggtgccc
     1801 gcgggtggtg gtctcggcac agctgatccc gacgtggtcg gcgccggcca accgcaggtc
     1861 cacaccagcc ctccgggcgg ctgccaccac ctcggccgcc cggccaggca ccctggccag
     1921 caccgtgtcg aagaactcct cagacaccac ctcgacaccg ccggcgcgca acccggcggc
     1981 cagcaccgcg gccatccggt gcgcccgccg tgcgatgtcg cgcagcccgt caggcccgtg
     2041 gtagatcgcg tacatcgagg ccatcacggc cagcagcacc tgcgctgtgc agatgttgct
     2101 ggtcgccctc tcccggcgga tgtgctgctc gcgggtctgc agcgccagcc ggtacgccgg
     2161 gtttccatca gcgtcccggg acatccccac caggcgaccc ggcatctgac gttccaggcc
     2221 gtgacggacg gcaaggtagg cggcatgcgg tccaccgaag ccgatcggca ccccgaagcg
     2281 ctgggtggag ccgaccgcga catcggcgcc gatctcgcct ggtggacgga gcagagtcag
     2341 cgccagcggg tcggccgcga cggctaccgc ggcgcctcgt tggtgcgcct gctcgatgag
     2401 catcgcgtgg tcgcgcaccg caccggaggc accaggatag gacagcaaca cgccgaagaa
     2461 ctcaccgtcc gggagactcg gccccgaacc tttcccgaac acgtcggcga catccagttc
     2521 gatgcccagc ggctcggcac gggtggcaag cacggccaaa gtctgtggca gcgtatcggc
     2581 gtcgacgacg aatcgggatg agctggtccg gccggcccgg tgcagcaggg tcatcgcctc
     2641 ggcggcggcg gtggcctcgt cgagcaacga agcattcgct accggcaagc cggtgaggtc
     2701 ggccaccacc gtctggaagt tgagcaacgc ttcgagccgg ccctgggaga tctccggctg
     2761 gtaaggcgtg taggcggtgt accaggccgg gttctctagc accgaacggc ggatcaccgg
     2821 cggggtgacc gtgccgtggt atcccatccc gatcatcggt atccgtgggt gacagcgggc
     2881 ggctcgcgca cgtaactcag tcagtgcctg ttcttcggtg gccggagccg gcaggttcga
     2941 ggcggcgtac gggtcgacca cggtcggcgg cagggcctgc tgcgccagtt cgtccaacga
     3001 ttccacgccg atcgtggcca gcatccgagc cagctcgccc ggccgcggcc cgatgtgccg
     3061 gtcggcgaac ggtgtgccgt gctccagctc agacagcggg cgccggtcag atggattcat
     3121 cgcggtgacc tccgggcctc agctcgaacc gtcgggcgcc gtgtccgatt ggtcctcccc
     3181 ctctgtcact acccagaccc cgtcctgccg agaactgggc gcctgagaga ttcaccggct
     3241 tgcgccggct ttccccgtcg gcgggtagcg tgcgctaccg ctttccagag gtgtctgacc
     3301 cgtgcggtcc tgttcgcctg agaggtt
//
LOCUS       DATADN010001038         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_730196, whole genome shotgun sequence.
ACCESSION   DATADN010001038 DATADN010000000
VERSION     DATADN010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730196"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VHH53_13340"
     CDS             <1..885
                     /locus_tag="VHH53_13340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2291145.1"
                     /translation="DGHPRAGLAWVPISDIRGDRLSSLPFSDRAEPLIDDEVDWKALA
                     EGVITATAPLTLRCFDTAVPVDDPRLRQVATAAWHGTPLDGSIEEIRQRLSQHARRNI
                     GISERAGVRIEAHTGADAVRRFHGLHVALRKRKYRLLAQPCELFDHIWEAFSPGDAIV
                     TMLAYAGDELIAGAVFLTWNGVLYYKFGASCHEHLALRPNDAIFWAGIRWGIERGARL
                     LDWGLSDLDQPGLIAYKRKWATEQRTIVTLRAGGEQARTGSDAGPMLAELTRLFTDDS
                     VPDEVTAQAGTLLYRYFC"
     gene            complement(815..1408)
                     /locus_tag="VHH53_13345"
     CDS             complement(815..1408)
                     /locus_tag="VHH53_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291146.1"
                     /translation="MTAVTVSVLAIPRLPRRARAALLTVHVAVSVGWLGLDGALVALE
                     VTSLTSGDPAVQGGIEAAMGALASWVLIPVVFTSLVSGLVLALSTPWGLVRHWWVLAK
                     CGIAVTLTATGLALMLPRLGQVAIEDGEPIQLQTLLVRSLALVLLLAATGLSVIKPWG
                     KTPHGRAAQVTAKRVSRSSDTEACRPVRSPRPVPSRR"
     gene            complement(1441..2097)
                     /locus_tag="VHH53_13350"
     CDS             complement(1441..2097)
                     /locus_tag="VHH53_13350"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX2291147.1"
                     /translation="MSSGVEQVRLPARWEPAARRVVDLTVVLVALLVLAIPMLLVAMA
                     IRLGTPGPALYRQQRIGRGGQPFTMYKFRTMRVGGSDAQHRDLIARELRGEDTSVGGS
                     WKIDSDPRVTRLGSILRRTSADELPQLLNVLRGQMALVGPRPCLDWEAKMFPPKYAGR
                     FDVPPGLTGLWQVSGRSTMGTLEMLELDLTYVRSWSFWTDLTILVRTVPTLLRGHGAR
                     "
     gene            complement(2081..3538)
                     /locus_tag="VHH53_13355"
     CDS             complement(2081..3538)
                     /locus_tag="VHH53_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291148.1"
                     /translation="MTQTLPPGSSPNPVSSPDSGSREIRPIPEISGWLGWGAAGCAVV
                     GGVVAVAVGPLIVFTVIGVLALVGLLAVAAYRPIFATYAYLGTLPIIAGIDRGNLIPL
                     VRPNEALLAVLLAGALAGGYVRWCRGDRLPLQLHKLDIPIGVFLLMSTAWPLLSLILR
                     GQTPVTADLAAVLPVCKLVAIYLLVRFSVTSEAQLVRCFRLIIWPGAVVALIAILQTL
                     NFGPVVAMLQAVWTPDSHAAALAARGTTTLGSPLATGDYIIICLTLVICCAVRGLLHY
                     RERLVLGAVLGAGVLAAGQFSTWISAVVVAGIILWRFSTVRRGSMRFLWLVPLALAFG
                     APALIGRLQGFSEYGVPRSWIGRWNNLSDHYLPRFDFINVLIGVSPNSVLPAPERWRE
                     VIYLEGGYLQLLWVGGIPLLAAFVWLAVAVLRRSRELMEQPGAVGVGASALWVCWWFV
                     IILTVIDPHLTLRGTGDVLFMLMAITTGRLSVQRR"
BASE COUNT          687 a         1259 c         1146 g          502 t
ORIGIN      
        1 gacgggcatc cgcgcgcggg gctggcctgg gtgccgatca gcgacatccg cggtgaccgg
       61 ttgtccagcc tgccgttctc cgaccgggcc gagccgctga tcgatgacga ggttgactgg
      121 aaggccctcg ccgagggcgt catcaccgcc actgctccgc tgacgctgcg gtgcttcgac
      181 accgccgtgc cggtggatga tccgcgcttg cgccaggtcg caacggcggc gtggcacggt
      241 acgccgctgg acggcagcat cgaggagatc cgccagcggc tgtcccagca cgcccgccgc
      301 aacatcggga tctccgaacg ggccggcgtc cggatcgagg cgcacaccgg cgcggacgcc
      361 gtgcgccggt ttcatgggct acatgtcgcg ctgcgcaagc gcaagtaccg gctccttgcc
      421 cagccctgcg agctgttcga ccacatctgg gaagccttct ctcccggtga cgcgatcgtg
      481 acgatgctcg cgtacgccgg cgatgagctg atcgccggcg cggtcttcct gacctggaac
      541 ggcgtgctct actacaagtt cggcgcgtcg tgccacgagc acctggcact gcgcccgaac
      601 gacgcgatct tctgggccgg gatccgctgg ggaatcgagc gcggcgcccg gcttctcgac
      661 tggggactca gcgacctcga tcagccggga ttgatcgcat acaagcgcaa gtgggcgacc
      721 gaacaacgca ccatcgtgac acttcgcgct ggcggggagc aggcccgcac cggctccgac
      781 gccggcccga tgctggccga gctcacccga ctattcaccg acgactcggt accggacgag
      841 gtgaccgcac aggccggcac gcttctgtat cgctacttct gctgacgcgc ttggcagtga
      901 cctgcgccgc ccggccgtgc ggtgtcttgc cccatggctt gatcaccgag agaccggtcg
      961 ccgcgagcag gagaaccagc gctagcgacc tcacgagcag cgtctgcagc tggatcggct
     1021 caccgtcttc gatggctacc tgccccagcc gaggcagcat caaagccagt ccggttgcgg
     1081 tcagcgtgac agcgatcccg cacttggcga gcacccacca gtgccgcacc aggccccatg
     1141 gagtggacag cgcgagcacc agcccactga ccagtgaggt gaacacgacc ggaatcagca
     1201 cccaggaggc cagcgcgccc attgctgcct caataccacc ctgcaccgcc ggatctccac
     1261 tggtcaggct agtcacctcc agtgctacca gcgcaccgtc aaggccaagc catccgacgg
     1321 agaccgcgac gtggacggtc agcagggccg cccgggcccg gcgtggtagt cgcgggattg
     1381 ccaaaaccga cacagtgacg gcagtcaaaa gtccccctcc tgccagcaga cagtcctcgg
     1441 tcagcgtgcg ccgtgtcccc tcagcagggt gggcacagtt cgcaccagga tcgtgagatc
     1501 ggtccagaaa ctccagctac gcacatacgt gagatccagt tccagcattt ccaacgttcc
     1561 catcgtgctg cgcccactga cctgccagag tccggtgagc ccgggtggaa cgtcaaagcg
     1621 cccggcgtat ttgggcggga acatcttcgc ttcccaatcc aggcacgggc gcggcccgac
     1681 cagcgccatc tgccctcgca ggacgttgag tagctgggga agctcgtcgg cgctcgtgcg
     1741 ccgcaggatc gagccgagcc gggtcacccg cgggtcgctg tcgatcttcc agctgccgcc
     1801 gaccgacgtg tcctcgcctc gcagttcacg agcaatgaga tcccggtgtt gggcgtcgga
     1861 gccacccacc cgcatggtgc ggaacttgta catcgtgaac ggttggccac cccggcctat
     1921 ccgctgctgg cggtacagcg ccgggcccgg cgtccccagc cggatggcca tcgcgacgag
     1981 tagcatcggg atcgcgagca ccagcaacgc gaccaagacc accgtgagat cgaccacgcg
     2041 ccgggcggcc ggttcccaac gagcgggtaa gcgcacttgt tcaacgccgc tggacactga
     2101 gcctccccgt ggtgatagcc atcagcatga aaagcacgtc ccctgtgcct cgaagtgtca
     2161 ggtgcggatc gatgacggtc aggatgatca cgaaccacca gcagacccac agcgccgaag
     2221 ctccgacacc gaccgcgccc ggctgctcca tgagctcccg gctgcggcgc agcaccgcga
     2281 ccgccagcca gacgaaagcg gccagcaacg ggataccgcc gacccaaagc aactgcaggt
     2341 agccgccctc caggtagatc acctcgcgcc acctctcggg tgccggcagc accgagttgg
     2401 gcgacacccc gatcaggacg ttgatgaagt cgaacctcgg caggtaatgg tcggacaggt
     2461 tgttccagcg accgatccag ctacgtggca ccccgtattc actgaatccc tgcaaacggc
     2521 cgatcaacgc cggcgcgccg aaggcgagcg caagcgggac aagccacaga aatcgcatcg
     2581 agccgcggcg caccgtgcta aagcgccaca ggatgattcc ggccaccacg accgcggaga
     2641 tccaggtaga gaactggcct gcggccagca cgcccgcccc cagtacggct cccagtacca
     2701 accgctcccg gtaatgcagc agtccccgaa ccgcgcagca gatcaccagg gtcaggcaga
     2761 tgatgatgta gtcaccggtg gccagcggcg agccgagcgt cgtggtgccc cgggcggcga
     2821 gtgcagccgc gtggctgtcg ggcgtccaca cggcctgcag catcgccacc accgggccga
     2881 aattcagggt ctgcaggatg gcgatcaggg cgaccacggc ccccggccag atgatcaacc
     2941 ggaagcaccg caccagctga gcctcgctgg tgacggaaaa gcgcaccagc aggtagatgg
     3001 cgacgagttt gcagaccggc agcacggccg ccagatcggc ggtcactggc gtctggccgc
     3061 gcaatatcaa tgacagcagc ggccacgccg tcgacatcaa aaggaagaca ccgatcggaa
     3121 tgtcgagctt gtgcagttgc aggggaagcc gatcaccgcg gcaccatcgg acgtagccgc
     3181 cggctaacgc cccggcgagc agcaccgcca gcagcgcctc gttggggcgc accagcggga
     3241 tcagattgcc acggtcgata ccggcgatga tgggcaaggt gcccaggtag gcgtaggtag
     3301 cgaagatcgg ccggtacgcc gccacggcga gcaaccccac cagggccagc actccgatga
     3361 cggtaaagac gatcagcggc ccgaccgcca ccgcgacgac tccgccgacc acggcacagc
     3421 cggcggctcc ccatcccagc cagccgctga tctccgggat cggccggatt tccctggagc
     3481 cggaatctgg gctggaaacg ggattcgggc tggagccggg cggaagcgtt tgggtcatga
     3541 cccaccaccg atccgtcgcg gcatcgcgac acgaagtgtc aggacataga cccc
//
LOCUS       DATADN010001039         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_437965, whole genome shotgun sequence.
ACCESSION   DATADN010001039 DATADN010000000
VERSION     DATADN010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437965"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1230
                     /locus_tag="VHH53_13360"
     CDS             103..1230
                     /locus_tag="VHH53_13360"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2291149.1"
                     /translation="MFYLLKAQGLDPWLVNAKDVKHLPGRPKTDKLDAVWLCKVAERQ
                     MIRPSFVPPPEIRRLRDVTRYRIDLVGVRTAEKQRVEKLLEDSQIKLSVVASDIFGVS
                     GREMLAALIGGQTNPQALARLARGRLRAKIGALEEAFTGHFTDHHALLLSKMLARIDA
                     LDADIAELDAAIEEMVAPFASAVERLDEIPGVGITAARVIIAEVGLDMSRFPTAAHLA
                     SWARFAPGVKESAGRTKGSSTTGYGNRYLARALGEAAVSAGKTNTFLGERYRRIAKRR
                     GKNRAIVAIGRSILVIVWHLLSEPQARFIDLGPDFYDTRIGPERKKRNHIPPTRSAGL
                     QGHPGAHGLTTACPSRLRDTTTPSRWFRSPVPGSIFGSDIR"
     gene            1741..1905
                     /locus_tag="VHH53_13365"
     CDS             1741..1905
                     /locus_tag="VHH53_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291150.1"
                     /translation="MMPLLGAVTALPVGPRRVLVAGTAGAGKTTVARRGAALLDIPHI
                     EIDALFHGPS"
BASE COUNT          354 a          652 c          651 g          363 t
ORIGIN      
        1 aacccggccg cgacagacac gccgcccact caaaacatcc ctcaccttcc atggcggacg
       61 cctccctccc gaccccggat aacctcggat tactggaagc cggtgtttta tctgctgaag
      121 gctcaggggc tggatccctg gctggtcaac gccaaggacg tcaaacacct gccgggacgg
      181 cccaagacag acaagctgga cgcggtgtgg ctgtgcaagg tcgccgagcg gcagatgatc
      241 cgcccgagtt ttgtgccgcc gcccgagatc cgccggttgc gtgatgtcac tcggtatcgg
      301 attgatttgg tgggggtgcg aacggcggag aagcagcggg tggagaagct gctggaggat
      361 tctcagatca aactcagtgt ggtggcctcg gacattttcg gagtctcggg gcgggagatg
      421 ctggcggcgt tgattggtgg gcagaccaat cctcaggcgt tggcgcggct ggcccggggt
      481 cggctgcgcg ccaagatcgg ggcgttggag gaggcgttca ccgggcattt caccgaccac
      541 cacgccttac tgctgagcaa gatgctggcc cgcatcgacg ccctggatgc cgacattgct
      601 gagcttgacg ccgcgattga ggagatggtc gcccctttcg cctcagcggt ggagcgcctt
      661 gatgagattc ccggggtcgg gatcaccgcg gcgcgcgtga tcattgctga agtcgggctc
      721 gatatgtccc ggttccccac cgctgcccac ctggcttcct gggcccgttt cgccccgggt
      781 gtgaaggaat cagccggtcg caccaagggc agcagcacga ccgggtatgg caaccgctac
      841 ctggcccgcg cgctcggaga ggccgccgtg tccgccggca agaccaacac cttcctcggc
      901 gaacgctacc ggcgcatcgc caaacgccgc ggcaagaaca gagcgatcgt cgccatcggt
      961 cgctccatcc tggtcatcgt ctggcaccta ctgtctgaac cccaggcccg ctttatcgat
     1021 ctcggacccg atttctacga cacccgcatc ggcccggaac gcaaaaaacg taaccacatc
     1081 ccgccaactc gaagcgctgg gctacaaggt caccctggag cccacggcct gaccacggct
     1141 tgcccgtccc ggctccgcga cacaacaacg ccgagccggt ggttccgttc gcccgtcccc
     1201 ggctcgattt tcggatcaga cattcggtga tggtcgcccg ggtagccccg ccaccctcgt
     1261 tgccgtcatc gacgccacca tttgcggttt cgtggccacc gggtcagcta gggatgagga
     1321 tgcggccgac acaggcgagg tgtacgccat ctacgtcgac ccgcaggctt ggggacgggg
     1381 aatcgggcga tcactgatag ccgaagcccg aactcgacta agccgctagg gattcacaga
     1441 agccgtcctg tgggttcacg ctggtgatga acgagcccaa cgcttttatc gggccgacgg
     1501 atggcagtcc gacgggcacc ggcgtcaaga atacgtgtgg ggcgcgctgg ccgacgaagt
     1561 ccgttatcgt cgctgtctgc cttaatgccg accgcactga tttttcactg ccgcggatgt
     1621 atccgcacat gccgcgagga gcgcggccat gctgatctgg tgctgtctgt gcgcgggtgc
     1681 cggtgaaggc ccctgctgag cggcccgtac ggggacacaa cgagccaagg ttaatatcgg
     1741 atgatgcccc tgctcggcgc cgtgacggcc ttgcctgtgg gtccgaggcg ggtcctggtg
     1801 gctggcacgg ccggcgcagg caagacgacc gtcgcgcgtc gcggcgcggc gctgctcgac
     1861 attccgcata tcgagataga cgcgctcttc catggcccga gctgaacacc ccaagacacc
     1921 tttgagtcgg aggtccacag gttcgtgcgt gtccgtgctg ggtgacgaaa tggcagtact
     1981 ctcaggtccg cgcgatcctc gcgcagcgag ctgacctctg
//
LOCUS       DATADN010001040         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1322693, whole genome shotgun sequence.
ACCESSION   DATADN010001040 DATADN010000000
VERSION     DATADN010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322693"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /gene="recN"
                     /locus_tag="VHH53_13370"
     CDS             <1..1630
                     /gene="recN"
                     /locus_tag="VHH53_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEX2291151.1"
                     /translation="AVVEGRLRPPEGSSAADLAKEVGAQTDEDGSLIASRTLTADGRS
                     RAHLGGRSVPAGVLADLADGMLAVHGQNDQLRLLRPGEQRAVLDRFAGDVVFGPLEAY
                     RRIRQEWLHCCAELTTRRDRSRELAREADLLGHGLAEITAIDPQPGEDGALIVEARRL
                     ADADQLREAAAIAQSAISGAFDPDPDQPGAFGLVGQARRQLQTVQDPALQALVPHLVE
                     VAALLGDVGAELGGYLEGLDSDPARLEQVLTRQAELKGLTRKYAADVDGVLAWARHAR
                     QRLAGLDTSEAAMASLAARRDELADELARHATAVTTARTAAARELSDAVTAELAGLAM
                     SQAQLSVAVQPVLAGDGDAAGLTVAGRRLRAGPDGTDEVELRLAPHPGAEALPVHKGA
                     SGGELSRVMLALEVVLVDADPVPRMVFDEVDAGLGGRAAVEIGRRLARLARSHQVVVV
                     THLPQVAAYADRHLVVDKATYATGLTRSGVRQLEGVARVSELARMLAGLDDTDTGRAH
                     AEELLSSATADKSKLAGCGAPPLAAARVLPPSPA"
     gene            1627..>1886
                     /locus_tag="VHH53_13375"
     CDS             1627..>1886
                     /locus_tag="VHH53_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphokinase"
                     /protein_id="HEX2291152.1"
                     /translation="MRISGLLNRPGHELPGVSGLAKVDRRADALVRRVGPGDIAVLDQ
                     VDLDRRTADALVAAGVAGVVNAAPSISGRFPNLGPEILVAAG"
BASE COUNT          258 a          645 c          700 g          283 t
ORIGIN      
        1 tgccgtcgtg gagggtcgcc tccggccacc cgagggcagc tcggcagcgg acctggcgaa
       61 ggaggtgggt gcccaaaccg atgaggacgg cagcctcatc gccagccgca cgctcaccgc
      121 agacggtcgc tcccgcgcgc acctcggtgg acgctcggtc ccggcgggag tgctggccga
      181 cctggccgac ggaatgctcg cggtacacgg tcagaacgat cagctcaggc tgctgcgacc
      241 cggtgagcag cgggccgtgc tggaccgctt cgccggcgat gtggtgttcg ggccgctgga
      301 ggcctaccgc cgcatccgcc aggagtggct gcattgctgc gctgagctca ccacccggcg
      361 ggatcgatct cgggagctgg cccgggaagc agatctgctc ggtcacggac tcgccgagat
      421 caccgctatc gacccccagc ccggggagga cggtgcgttg atcgtcgagg ctcggcggct
      481 tgccgacgct gatcaactgc gtgaggccgc cgccattgcg cagtccgcga tcagcggtgc
      541 gttcgacccg gaccccgacc agccgggagc gttcggtctg gttgggcagg cccgccgcca
      601 gctgcagacg gtgcaggatc ctgcgctgca ggcgctggtc ccccacctgg tggaagtcgc
      661 ggcgctactt ggtgatgtcg gtgccgaact cggcggttac ctggaggggc tggattccga
      721 cccggcccgt ctcgagcagg tgctgacccg ccaggccgag ctcaaggggc tcacgcgcaa
      781 gtacgccgcc gacgttgacg gtgtgctggc atgggcacgc cacgcccgcc agcgcctggc
      841 cggtctggac acctccgaag cagccatggc ctcgctggcg gcccggcgtg acgaactggc
      901 cgatgagctg gcccgccatg ccaccgccgt cactacggcg aggacggcgg ccgcccgcga
      961 gctctccgac gccgtcaccg ctgagcttgc ggggctggcc atgagtcagg cgcaactgtc
     1021 cgtggcggtc cagcctgtcc tggcagggga cggtgacgcc gccggactca ccgtggccgg
     1081 gaggcggttg cgggctgggc cggatggcac cgatgaggtg gagctgcgcc tggcccccca
     1141 tccgggcgcc gaggcactgc cggtgcacaa gggcgcgtcc gggggagagc tgtcccgggt
     1201 gatgctggcg ctggaggtgg tgctcgtcga cgccgatccg gtaccgagga tggtgttcga
     1261 cgaggtcgac gccggtctcg gcggacgcgc cgcggtggag attggacgcc ggttggcccg
     1321 gctggcccgc agccatcagg tcgtggtcgt cacccacctg ccccaggttg ctgcctacgc
     1381 ggatcggcat ctcgtggtgg acaaggcaac ctacgccacc ggcctgaccc gtagcggggt
     1441 ccggcagctc gagggagtgg cccgcgtcag cgagctggcc aggatgttgg ccggactgga
     1501 tgacaccgac accgggcgtg cccacgccga ggagctgctc tcgtccgcga ccgcggacaa
     1561 gagcaagctg gctggctgcg gcgcgcctcc cctggcagcg gcgcgggttt tgccaccatc
     1621 accggcatga gaatctccgg cctgctcaac cggcccggcc atgagctgcc cggcgtcagt
     1681 gggctggcca aggtggaccg ccgcgccgac gcgctggtac gccgcgtcgg tcccggcgat
     1741 atcgcggtgc tcgatcaggt cgatctggac cgcagaaccg ccgatgcgtt ggtggccgct
     1801 ggcgttgctg gggttgtcaa tgccgcaccg tcgatctccg gccgattccc gaacctgggc
     1861 cctgagatcc tggtggcggc gggcat
//
LOCUS       DATADN010001041         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_112907, whole genome shotgun sequence.
ACCESSION   DATADN010001041 DATADN010000000
VERSION     DATADN010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112907"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(697..1014)
                     /locus_tag="VHH53_13380"
     CDS             complement(697..1014)
                     /locus_tag="VHH53_13380"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HEX2291153.1"
                     /translation="MTMRARLAAPVGEVVWDINLFLRGWAGYFRYGNSAHAFGKIRKY
                     ALMRLALFVAKRHQRGRAWGFNLLYRSPNDLGLISLNGIVVAPRPYNAWRVKPNTAGE
                     EHR"
     gene            complement(1011..2066)
                     /gene="ltrA"
                     /locus_tag="VHH53_13385"
     CDS             complement(1011..2066)
                     /gene="ltrA"
                     /locus_tag="VHH53_13385"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX2291154.1"
                     /translation="MSALVAGPTPGVGSPGAVRALQHALYRAAKADPGRRFHALYDKV
                     HRRDVMWRAWGQVRRNNGAPGVDQTTLTDVEQYGVTRLLGELEAELKAGAYRPLPARR
                     VFIPKPGSDERRPLSVPSVRDRIVQAAVKIVLVPVFEADFLPCSFGFRPKRSAHDGLQ
                     VLVDESFAGSRWVVETDVADCFGSIPHSGLMSAVEERVSDRRLLALLRSFLRAGVMEQ
                     GSVRRPVTGSPQGGVVSPLLANVYLHRLDRAWRGAYGTLARYADDALVMCRSKGQADA
                     ALARLTALLAELGLKPKLAKTRIVKLEVGGEGVDFLGFTTGWCATGPDEDRTASPSWP
                     AGPHARRCSTPETASGS"
     gene            2759..2890
                     /locus_tag="VHH53_13390"
     CDS             2759..2890
                     /locus_tag="VHH53_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291155.1"
                     /translation="MTVRLLYLIFRQLVAWFGLLARSARSKNAEILVLRHEVAVLRY"
BASE COUNT          516 a         1081 c          966 g          557 t
ORIGIN      
        1 cgacggcaaa cgtgacgcaa tcatgcagaa gttggcgaac gaagaacaag gcagcacgtc
       61 gtactggcct gcgactccga agccccggcc aaggcccgac tggcccatcg gtaactttcg
      121 ggagttgcgg ggatcgtcgc ctagctgtgt ccgttggccg atcctctgct gggactcgct
      181 gcctgtcgtt gcttgattgt ctattcgggc atgttcggcg gcggcgtcat tgaaccggtc
      241 ggtgacggtt gcgcagggag atcaccgatt tggtgtccga gctggtcgag cacctcgcga
      301 aagatcttgt agcgctcttc cccgacctcg ttgccaccgc gcctcgacct ccgccatcgc
      361 ggcgcggatg gcgcttcgag ctcgctcacc gcgcgcggtg cagcgaatga ccttggctct
      421 tcgattggtc ggatcctgaa cgatgtccac gtagccgcgg tcggcgagct ggctggcgag
      481 ctgaccaagc ggcaccgttg cataaggcga agggtgtcct tgccgaggat ggttgggtcg
      541 ggagctggcg cggtggcagt ggctgactgg taggacccgg ggcctgtgtt ccgccggttt
      601 cccggtcggg tgtgccacct cggtggccat tggtcagcgt gctccgtttc cagctcccgc
      661 ccgtcgatcc gtgcatgcgg ttttcccgca cacggctcac cgatgttctt caccggcggt
      721 gttcggcttg acccgccagg cgttgtaggg cctgggtgcg acgacgatgc cattgaggga
      781 gatcaggcca agatcgttgg gcgatcggta gagcagattg aagccccaag cccggccgcg
      841 ctggtgccgc ttggccacga acagggccag ccgcatcagc gcatactttc taatcttgcc
      901 gaaggcgtgg gcggagttcc cgtaccggaa atagcccgcc cagccgcgca ggaacaggtt
      961 gatatcccac acgacttctc cgaccggtgc ggccagcctg gcccgcatgg tcatgaaccg
     1021 gatgcggtct cgggcgtgct gcatcgcctt gcgtgagggc cagcgggcca ggaaggtgaa
     1081 gctgttcggt cttcgtctgg accggtagcg caccagccgg tggtgaagcc gaggaagtcc
     1141 actccctcgc cgcccacctc cagcttcact atccgggtct tggccagctt cggtttcagg
     1201 ccgagctcgg cgagcaatgc ggtcaaccgc gccagcgcgg catctgcctg ccccttggac
     1261 cgacacatca ccagcgcgtc gtcggcgtaa cgggccagcg ttccgtaggc gccacgccat
     1321 gcccggtcca gccggtgcag atagacgttg gcgagcaacg gggataccac cccgccctgc
     1381 ggactgccgg tgaccggacg ccggaccgat ccctgctcca tcactcccgc gcgcagaaac
     1441 gagcgcagca gcgcgagcag tcgtcggtca ctgacgcgtt cctcaaccgc ggacatcaac
     1501 ccggagtgcg gaatcgagcc gaaacagtcg gccacgtccg tctccaccac ccagcgagaa
     1561 cccgcgaacg actcatccac gagtacctgc agaccatcgt gcgccgagcg tttaggccga
     1621 aacccgaacg aacacggcag aaaatccgct tcaaaaaccg gcacgagcac gatcttcacc
     1681 gcagcttgca cgatccggtc gcgaaccgaa ggaaccgaca gaggcctccg ttcgtcgctg
     1741 cccggcttcg ggatgaacac ccgccgggcc ggcaacgggc gatacgcccc ggccttcagc
     1801 tccgcttcca gctcaccgag cagccgggtg accccgtact gctccacgtc ggtcaaggtg
     1861 gtctggtcaa caccgggagc gccgttgttg cggcgcacct ggccccacgc ccgccacatg
     1921 acgtccctgc ggtggacctt gtcatacagc gcgtggaatc gtcgtccggg atcggccttg
     1981 gccgcccggt aaagcgcatg ctgcaaagca cggaccgcgc cgggactccc gacgccaggg
     2041 gtgggaccag ccacgagggc actcaccggc cactccttga tcaacctacg cgtgaacgaa
     2101 gcagcggccc ttcccatcac cggcggttgt gttgtccgtc cggctcgatc ggtactacga
     2161 ccgcctccga cgccgttccg gccgatcgtc cacttcccgg ctcctcaccg gttataagac
     2221 gccgctttcc ggtgcgcttc cgcagccacc gggccgggga cggcctcccc agttcccgcc
     2281 atcaccttct gaacgttcca cgccctctac gccggggagt tcctcgcggc tgtgctccag
     2341 gttctgcacc gcttccttgg ccttcaccct gagggacggg gctcggctct ccctgatccc
     2401 gccattagcg ggcacattca cggcgcggca ggcttcgctt catgctgcgg accgctcagt
     2461 tgctccccct tgaagggctc ttgacaccgc acttcgacgc cgggcgtttc cccccgacac
     2521 cggcagcctg ctaccgggcc ccctggcggc tacccggacc ggactctcac cggctggcga
     2581 cgacgagctt cagaccgaat cagatcacgt caccatcggc gcaaccccct tcggtcactg
     2641 ggcatacccc cgggactcgt ggaacttgag gtggcggtcc tgtgggtgcc gcataggtca
     2701 tccacggtga tgacctgcgg catcactagc caatggccgt caggcatgat cgtcagccgt
     2761 gacggtacga ctgctttatc tgatcttccg acagcttgtg gcctggttcg gattgctggc
     2821 ccgcagcgca cgatcgaaga acgcggagat cctggtgctc cggcatgaag ttgccgtgct
     2881 ccggtactag gtcagacgac cgcgactgtc gtgggcggat cgggcggtgt tcgcagcgct
     2941 gacccaggtg ctatctaggg cctgccaact gcatcggatc gtcacccccg ccacgatctt
     3001 gcgatgcacc gtgatctagt gaaacgacgc tggacccatc cccgacaccg caccggaggc
     3061 cgctccacgg cacccgggct ccgccaactg gtcctgcgcc tggcctcgga gaatctgacc
//
LOCUS       DATADN010001042         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_585025, whole genome shotgun sequence.
ACCESSION   DATADN010001042 DATADN010000000
VERSION     DATADN010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585025"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1421)
                     /gene="glp"
                     /locus_tag="VHH53_13395"
     CDS             complement(204..1421)
                     /gene="glp"
                     /locus_tag="VHH53_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX2291156.1"
                     /translation="MAVAQKPRSVDEHSRVVAALLSATPVIRLPLVECAGLALAEPVT
                     ATLPLPPFDNSAMDGYAVRSVDVADATADKPVQLPVREDIPAGRTDSPTLQPGTAHRI
                     MTGAPMPPGADAVVPVELTDAGLHQVRIFAPRSTGQSVRRAGDDVATGDEVLAAGTVL
                     GPAQLGVAAAVGQAELPVHRRARVLVLATGSELVTPGTPLQHGQIYESNSTMLAAAVR
                     EAGGEAQLMHFVPDDVDELEAALAARLDGIDLLVTSGGVSAGAYEVVKDAMTGHGVEF
                     GKVAMQPGGPQGAGRYRGVPVATLPGNPVSSSVSFELFVRPALRAAMGLPAQRPRVIA
                     RLTEALESPPDLRQFRRGALDATSGTVAPVGGPGSHLLGALARSDCLIDIPESTTHLA
                     AGDEVVVLRTGGT"
     gene            1469..1618
                     /locus_tag="VHH53_13400"
     CDS             1469..1618
                     /locus_tag="VHH53_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX2291157.1"
                     /translation="MAASIAVHHSTIEMDGFRSLTAGERVSFEIAAGRDGRDQATAIR
                     KLPGS"
BASE COUNT          257 a          571 c          550 g          245 t
ORIGIN      
        1 ataaaaccgt tcgaccgctc cccagctttc cgaacggttt tcttccagcg cccctgcgcc
       61 ccccgacggc gcaggaacac tgcgcctctg ggaggacaac agctcgccga gcattgctta
      121 ctgttagtgt gctttggctc acccgccatc acaaggccat gctggacggt tgtggtttcc
      181 tcacacccgc aagatgtgac tgatcacgtg ccgccggtgc gcaatacgac tacctcatct
      241 cctgccgcga gatgcgtcgt ggactccggg atgtcgatca ggcagtcaga ccgggccagc
      301 gcgccaagca agtgtgagcc ggggccaccg accggcgcga cagtgcctga agtagcgtcc
      361 agcgcgccgc ggcgaaactg ccgaaggtcc gggggcgact ccagtgcctc ggtcagccgc
      421 gcgatgaccc gaggccgttg cgcaggcagg cccatcgcgg cccgcagcgc gggtcggacg
      481 aacagctcga aagacaccga ggaactcacc gggttgccgg gcagcgtcgc caccggcacc
      541 ccacgataac gaccggcgcc ttgcggaccg ccgggctgca tggccacctt gccgaactcc
      601 acgccgtgcc cggtcatggc gtccttgacc acctcatagg ccccggcgct gaccccgccg
      661 gaggtcacca gcaggtcgat accgtccagc cgggcggcca gcgcggcctc cagctcgtcg
      721 acgtcatcgg gaacgaagtg catcagctgt gcctcgccgc ctgcctcccg gaccgcggcg
      781 gccagcatcg tgctgttcga ctcgtagatc tggccgtgct gtagcggtgt gcccggcgtc
      841 accagctctg agccggtggc cagcactagc acccgggccc ggcgatggac cggtagttcg
      901 gcctggccga ccgcggccgc gacgccgagc tgggccggac ccaataccgt gccggccgct
      961 agcacctcgt ccccggtcgc cacgtcgtct ccggcccggc gcacactctg gccggtggac
     1021 cggggggcaa agatccgcac ctgatgcaat ccggcgtcgg tgagctccac cggcacgacg
     1081 gcgtccgcgc ctggtggcat cggcgcgccg gtcatgatcc ggtgtgcggt gcccggctgc
     1141 agcgtaggcg agtcggtacg cccggcaggg atgtcctcgc ggaccggaag ctgcaccggc
     1201 ttgtcggcgg tggcgtcggc gacgtccacc gagcgcacgg cgtaaccgtc cattgcggaa
     1261 ttgtcgaaag gcggcagcgg caacgttgcg gtgaccggct cggccagtgc caggccggca
     1321 cactccacca gtggcagccg gatcaccgga gtggcggaca gcagggcagc taccacccgg
     1381 ctgtgctcgt cgacggatcg gggtttttgc gctacggcca tccctgcatc ctgccggtag
     1441 tgggcgactg cgcgcgcgga ggtcagcgat ggctgcgtcg atcgctgtgc atcacagcac
     1501 gatcgagatg gacggcttcc gaagcctgac cgcgggtgag cgggtcagct tcgaaattgc
     1561 ggccggccgg gatggccggg atcaagccac cgccatccgc aagctgccag ggtcgtgagt
     1621 gcg
//
LOCUS       DATADN010001043         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_585038, whole genome shotgun sequence.
ACCESSION   DATADN010001043 DATADN010000000
VERSION     DATADN010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585038"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHH53_13405"
     CDS             <1..198
                     /locus_tag="VHH53_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEX2291158.1"
                     /translation="AGAALPVLLAFSLSGQSFGTLITAQNVAQEVVRTLVGSIGLVAA
                     VPITTAIAAVVVSRDHIASMQ"
     gene            complement(215..916)
                     /locus_tag="VHH53_13410"
     CDS             complement(215..916)
                     /locus_tag="VHH53_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2291159.1"
                     /translation="MLVAGFATGSFQANCFVLAAEAGGPCVVVDPGQDAEQPLGDMLR
                     KHRLDPVAVLLTHGHFDHIFAVTPVCDGHDIPAWIHPEDRVLLSDPMRALSADAQQFF
                     GGRVQLREPREVRALDDGTSLELAGLSFTVDHTPGHTRGSVTFRSLTDNGAGERQALL
                     VSGDTLFAGSIGRTDLPGGDHEQMLNSLRGKILVLDDDTIVLPGHGPQTTIGRERATN
                     PFLHGLAVPGRARGL"
     gene            1062..>1851
                     /locus_tag="VHH53_13415"
     CDS             1062..>1851
                     /locus_tag="VHH53_13415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2291160.1"
                     /translation="MPTNEQRREAAKRKLDRQLARRAQRAKSRRKYAVAGSVAAVALV
                     IGVVGLIVTLGGGDQTVNTAANPATPAAPVTTTATTKPSPSNELPAPLTRPQPLPASV
                     DCQYQATQQTPARPVTPPPAGPTSTEGSVAATMQTSAGPIGLTLERALAPCTVNSFIS
                     LARQGFYANSPCHRLVTNPGLQVLQCGDPTGSGAGGPGYTVPDEVFPELTYGRGYVAM
                     ANTGQPDSGGSQFFLIYGQTQLPPDYTVFATITPEGLKVIDEIAR"
BASE COUNT          324 a          653 c          607 g          267 t
ORIGIN      
        1 gccggtgccg cgttgcccgt gctgctggct ttctcgctgt ccgggcagag cttcggcacg
       61 ctgatcaccg cacagaacgt cgcccaggag gtggtgcgca cgctcgtcgg cagcatcggg
      121 ctcgtcgctg ccgtcccgat caccacagcg attgccgcgg tggtcgtctc ccgcgatcac
      181 atcgcgtcaa tgcagtagca agccgatcat caagtcacag accccgcgct cgaccaggga
      241 cggcaagacc gtgcaggaac gggttggtgg cacgttccct gccgatggtg gtctgagggc
      301 cgtgaccggg caggacgatg gtgtcgtcgt cgagcacgag gatcttgccc cgaagcgagt
      361 tgagcatctg ctcgtgatcg ccaccaggta ggtcagttcg cccgatcgag ccggcgaaca
      421 gcgtgtcgcc ggagacgagc agggcctgcc tctcaccggc cccgttgtcc gtcagcgaac
      481 ggaacgtcac cgaccctcgg gtgtgccccg gggtgtggtc gacagtgaag ctcagcccgg
      541 ccagctccaa ggacgtaccg tcgtccagcg cgcgtacctc gcgcggctcg cgcagctgga
      601 cccgaccgcc gaagaactgc tgcgcatcgg cggacagtgc tcgcatcggg tcggagagca
      661 gcacgcgatc ctcggggtgg atccaggccg ggatgtcatg gccgtcacac accggggtga
      721 ccgcgaagat gtgatcaaaa tgcccgtggg tgagcaacac ggcgaccgga tcgagccggt
      781 gcttgcgcaa catgtcgccc agtggctgct ccgcatcctg accggggtcg acaaccacac
      841 atgggccgcc cgcctcggcg gcgagtacga agcagttggc ttggaaggag ccggtggcga
      901 aacctgcgac gagcaccgtg actccacttc ggtgacggag atgggacgtc cccagcctag
      961 ggcagcgctc gccggggagg ctgacgtcgg gctcacctgc ggcccaccta gactacgcgg
     1021 cgttaaccgc cccatcacag ttgtcagcca caggagcctg ggtgcccacg aacgagcaac
     1081 gccgcgaggc agcgaagcgc aaactggacc gccagctggc tcgccgcgcg cagcgggcca
     1141 agtcgcgccg caagtacgcg gtagctggca gcgtggccgc ggtagcgctg gtcatcggtg
     1201 tcgtagggct gatcgtgacg ctgggcggag gcgaccagac ggtcaacacc gccgcgaacc
     1261 cggccacccc ggccgcgccc gtcaccacga ccgccaccac caagccttct ccgtcaaacg
     1321 agctacctgc cccgctgacg cggccgcagc cgctgcccgc ctcggtcgat tgccagtacc
     1381 aggccaccca gcagaccccg gccaggccgg tgacacctcc accggccggt cccacctcca
     1441 ccgagggcag tgtggccgcg accatgcaaa cctcggccgg cccgatcggc ctcaccctgg
     1501 agcgggcgct ggccccctgc accgtgaaca gcttcatcag cctggccagg caaggctttt
     1561 acgccaacag cccatgtcac cggctggtca ccaaccccgg gctgcaggtt ctgcaatgcg
     1621 gcgacccgac cggctcgggc gccggcgggc cggggtacac ggtgcccgac gaggtatttc
     1681 ctgagctgac ctacgggcgc ggatacgtcg cgatggccaa caccggccag cccgacagcg
     1741 gcggcagcca gttcttcttg atctacggtc aaactcagct cccgcccgac tacaccgtat
     1801 tcgccacgat caccccagag ggtctcaagg tgattgatga gatagcgcgg g
//
LOCUS       DATADN010001044         2172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_113145, whole genome shotgun sequence.
ACCESSION   DATADN010001044 DATADN010000000
VERSION     DATADN010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113145"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VHH53_13420"
     CDS             <1..1012
                     /locus_tag="VHH53_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX2291161.1"
                     /translation="APVRTTITVPDSVSMVALLGSADELLRLVEAEVEADIHVRGNEI
                     AITGQPADNAFAVHVFDELIALLGTGQALRPDSVRRVIGMLRSGGSERPADVLSLDII
                     SRRGRTIRPKTLNQKRYVDAIDAHTIVFGIGPAGTGKTYLAMAKAVQALQTKQVNRII
                     LTRPAVEAGERLGYLPGSLSEKIDPYLRPLYDALHDMLDPESIPKLMAAGTIEVAPLA
                     YMRGRTLNDAFIILDEAQNTTPEQMKMFLTRLGFGSKVVVTGDVTQVDLPGGQVSGLR
                     IVQHILDGVEDVHFAQLSSVDVVRHRLVTDIVDAYARWDAVQEGTGARPVPNRSDRRR
                     RR"
     gene            1021..1590
                     /gene="ybeY"
                     /locus_tag="VHH53_13425"
     CDS             1021..1590
                     /gene="ybeY"
                     /locus_tag="VHH53_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX2291162.1"
                     /translation="MGIEIANESGTAVHEASLAAAARFAMDRLGVSPLAELSMLLVGT
                     GVMADLHQRWMDQPGPTDVMAFPMDEAGDGGLLGGGRPDSPVGAPTLLGDIVLCPEVA
                     TEQAAAAGHSVLDELHLLTVHGVLHLLGYDHAEPAEEREMFALQDRILGEFRRAARAS
                     RRRSAQREADTRLLGAVGLEGPEESGPPG"
     gene            1606..>2172
                     /locus_tag="VHH53_13430"
     CDS             1606..>2172
                     /locus_tag="VHH53_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HEX2291163.1"
                     /translation="MLTSSLPLLVLATALVVAAGAFAAADAALGTVSRARVDALVRSG
                     RFGAGQLALVVADRPRHINLLLLLRLSCELTATVLVTVAAVRALQPTWLAVLAAGLAM
                     VVVTYVVVGVGPRTIGRQHPYRVGLVVAGSVRALGRILGPLSRLLILVGNAITPGRGF
                     PEGPFSSEVELREVVDMAGERGVVEAGEA"
BASE COUNT          312 a          736 c          776 g          348 t
ORIGIN      
        1 ggcccccgtt cgcaccacca tcaccgttcc cgactcggtc tcgatggtgg ctctcctggg
       61 ttcggccgac gagctgctcc ggctcgtcga ggccgaggtc gaggccgaca tccacgtgcg
      121 cggcaacgag atcgcgatca ccggccagcc ggcggacaac gcgttcgccg tccacgtctt
      181 cgacgagctg atcgcgctgc tcggcaccgg ccaggcgctg cggccggact cggtacgccg
      241 ggtcatcggg atgctccgca gcggcggctc cgagcgtccg gcggacgtcc tcagcctgga
      301 catcatcagc cgccgcgggc gcacgatccg gcccaagacg ctgaaccaga agcgttacgt
      361 cgacgcgatc gacgcgcaca cgatcgtctt cggcatcggc ccggccggca ccggcaagac
      421 ctacctcgcg atggcgaagg ccgtgcaggc gctgcagacc aagcaggtca accggatcat
      481 cctcacccgc cccgcggtgg aggccggcga gcggctgggc tacctgcccg gctcgctgag
      541 cgagaagatc gacccgtacc tgcggccgct gtacgacgct ctgcacgaca tgctcgaccc
      601 ggagtcaatc cccaagttga tggccgccgg aacgatcgag gtcgccccgc tggcctatat
      661 gcgcggccgc accctcaacg atgcgttcat catcctcgac gaggcgcaga acaccacgcc
      721 ggagcagatg aagatgttcc tcacccggct cggcttcggc tccaaggtcg tggtcaccgg
      781 tgacgtcaca caggtggacc tgccgggcgg tcaggtgtct gggctgcgga tcgtgcagca
      841 catcctcgac ggggtggaag acgtgcactt cgcgcagtta tccagcgtcg atgtggtccg
      901 tcaccggctg gtcaccgaca tcgtggacgc ctatgcgcgg tgggatgccg tccaggaggg
      961 cactggggca cgcccggtgc ccaaccgcag cgaccgccgc cggaggcggt gaccccgccg
     1021 gtgggcatcg agatcgccaa cgagtcgggc accgccgtcc acgaggcatc gctggcggcg
     1081 gcagcccgtt tcgcgatgga tcggttgggt gtcagcccat tggccgagct gtcgatgctg
     1141 ctggtcggca ccggggtgat ggccgatctg caccagcgct ggatggacca gccgggaccc
     1201 accgacgtga tggccttccc gatggatgaa gccggtgatg gtgggctgct gggaggcgga
     1261 cggccggaca gcccggtcgg cgcaccaacg ttgctcggcg acatcgtgct gtgcccggag
     1321 gtcgcgaccg agcaggccgc cgcagcgggt cactcggttc tcgacgaact gcatctgctc
     1381 accgtgcacg gcgtgttgca cctactgggt tacgaccacg ccgagccagc cgaggaacgc
     1441 gagatgttcg ctttgcaaga ccggattctc ggcgagtttc gacgcgccgc acgggccagc
     1501 cgtcgccggt cggcccagcg ggaggcggac acccgcctgc tgggagcggt gggtctggag
     1561 gggccggagg agtccggccc tcctggataa cctgcgggca ccgacgtgct gacgtcgtcc
     1621 cttccgctgc tggtgctagc caccgccctg gtggtagccg ccggggcctt cgccgcggct
     1681 gatgccgccc tgggcacggt gtcgcgggcg cgggtcgacg cgctggtgcg ctccggccgg
     1741 tttggcgccg gtcagctcgc gctggtcgtt gccgatcggc cccggcacat caacctgctg
     1801 ctgttgctgc ggctcagttg tgaactgact gccacggtgc tggtcaccgt ggcagcggtg
     1861 cgggcgttgc agcccacctg gctggcggtc ctggcggccg ggctggcgat ggtcgtggtt
     1921 acctatgtgg tcgtcggggt gggcccgcga accatcggcc gccaacaccc ttaccgcgtc
     1981 ggtctggtgg tggccggatc ggtccgggcg ctgggacgga tcctgggccc gctgtcgcga
     2041 ctgctcatcc tggtcggtaa tgcgatcacg cccggtcggg gattcccgga agggccattc
     2101 agctccgaag tcgaactccg cgaggtggtg gacatggcgg gagagcgcgg cgtggtcgag
     2161 gccggtgaag cc
//
LOCUS       DATADN010001045         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_270002, whole genome shotgun sequence.
ACCESSION   DATADN010001045 DATADN010000000
VERSION     DATADN010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270002"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VHH53_13435"
     CDS             complement(<1..1157)
                     /locus_tag="VHH53_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX2291164.1"
                     /translation="MSCASCAARIERKLNRLTGVSASVNYATEKARINFPDGVTPEEL
                     LATVVNTGYTAELALDEVTAPSAGSETDGIRALRERLVGSAVLAVPVIATAMVPALQF
                     SGWQWLSLLLTVPVAVWGALPFHRAAWRNLQHGAVTMDTLVSMGVLAAFLWSCYALIF
                     GSAGTLGVRHEFGLSAHQDAGGAQIYLEVAAGITVFLLAGRYFEARAKRRAGAALRTL
                     LDIGAREVSVLRDGVETRVPVDRLVVGDQFVVRPGEKIATDGEVVAGSSAVDESMLTG
                     EAVPIEVNPGSPVIGGAVNVSGRLRARATRVGADTRLAQMARLVEDAQNGKARVQRLA
                     DRVSTVFVPVVIVLALATLDFWLVTGAPTAAAFTAAIAVLIIACPCALGLAT"
     gene            complement(1243..2097)
                     /locus_tag="VHH53_13440"
     CDS             complement(1243..2097)
                     /locus_tag="VHH53_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291165.1"
                     /translation="MRTPVTLLAVFAGIVVIFGIAISLGESIVGSILPAPNRESGPRA
                     GGDTEPAAGPGGLAVASQGYVLRARTTRFTAGETTTFRFTVFGADGKPVTDFARHPDQ
                     RMSLLVVRRDITGYQRLHPLMAPDGTWSVPLRLDNPGSYRAFAEFLPESAKEPVILGL
                     DLDAPGLVEPGPIPKIAATAKVDGYTVIGTGDLVAGAVSQLWMHVIRDDVPVTDLDTY
                     LGNAGHLVVLREGDLAYLKVRPIAGPRRDTTIGFEVEAPAPGYHRMFMEFQHHGRVRI
                     AEFTVLAR"
BASE COUNT          370 a          758 c          705 g          316 t
ORIGIN      
        1 gtcgccagcc cgagggcgca cgggcaggcg atgatcagca cggcgatcgc tgcggtgaac
       61 gcggccgcgg tcggcgcgcc ggtgaccagc cagaagtcga gggtggccag cgccagcacg
      121 atgacgaccg gcacgaatac cgtcgacacc cggtcggcca accgctgcac ccgcgccttt
      181 ccgttctgcg cgtcctccac cagccgcgcc atctgagcca gccgggtgtc cgccccgacc
      241 cgggtggccc gcgcccgcag ccgaccggag acgttcaccg ccccgccgat caccggtgaa
      301 cccggattga cctcgatcgg cactgcctcg ccggtgagca tgctctcgtc gacggcggag
      361 ctgccagcca ccacctcgcc gtcggtggcg atcttctctc ccgggcggac cacgaactgg
      421 tcaccgacca ccagccggtc gacggggacg cgcgtctcga ccccgtcccg cagtaccgaa
      481 acctcccgag cgccgatgtc gagcagcgtg cgcagggccg caccggcgcg tctcttggca
      541 cgcgcctcga agtagcgacc ggccagcagg aaaaccgtga tgcctgccgc gacctcgagg
      601 tagatctggg ccccaccggc atcctggtgc gccgacagac cgaactcatg ccgcacaccc
      661 agggtgccgg cactgccgaa gatcagcgca tagcaactcc acaggaacgc tgccagcaca
      721 cccatcgaga ccagggtgtc catggtcacc gcgccgtgct gcaagttccg ccaggcggcg
      781 cggtggaagg gcaacgcacc ccacaccgcc accggcaccg tgagcaatag ggacagccac
      841 tgccatccag aaaattgcag agcaggcacc atcgctgtgg caatcacggg gacggccagc
      901 acggcactgc ccaccaaccg ctcgcgcagc gcccgaatgc cgtcggtctc ggagccggcg
      961 ctgggagcgg tcacctcgtc caaggcgagc tcggcggtgt agccggtgtt gactacggtg
     1021 gccaggagct cctcgggtgt cacgccgtcg gggaagttga tccgcgcctt ctcggtggcg
     1081 tagttgacgc tcgcgctcac cccggtgagc cggttgagct tgcgttcgat acgcgccgcg
     1141 caggatgcgc agctcatgcc gccgatcgcc agttcgatct ggtgagttgg ctcaccagat
     1201 ccggccacgg tggcggtgct gcggtcacgg acgttcgcca tgtcaccttg ccagcacggt
     1261 gaactctgcg atccgcaccc ggccatggtg ctgaaactcc atgaacatcc ggtgataccc
     1321 cggggccggc gcctcgacct cgaagccgat cgtggtgtcc cggcggggac cggcgatagg
     1381 ccgcaccttt aggtaggcca ggtcgccctc gcgcaggacc accagatggc cggcattccc
     1441 gaggtaggta tccaggtcgg tgaccggcac atcgtcgcga atcacatgca tccacagctg
     1501 cgacacggcg ccggcgacca ggtctccggt cccgatcacg gtataaccgt ccaccttggc
     1561 cgtcgccgcg atcttcggta tgggtcccgg ctcgaccaga ccaggtgcgt cgagatccag
     1621 gccaaggatg accggctcct tcgccgactc gggcaggaac tcagcgaacg cccggtaact
     1681 gcccgggttg tccagccgca gcggcacgct ccaggtgcca tcgggggcca tgagcgggtg
     1741 caggcgctgg tagccggtga tgtcccggcg cacgacgagc aggctcatcc gctggtcggg
     1801 gtggcgcgcg aagtcggtga ccggcttgcc gtcggcgccg aagacggtga atcggaaggt
     1861 ggttgtctct ccggcagtga accgggtggt ccttgcgcgc agcacgtaac cctggctagc
     1921 gacggccagg ccgccagggc cggcggcggg ctcggtgtca ccaccggcac gtggcccact
     1981 ctcgcgatta ggtgcaggga ggatgcttcc gacgatcgac tctccgagac tgatcgcgat
     2041 gccgaagatt acgactatgc cagcgaacac cgccagcagc gttacagggg tccgcatctg
     2101 ctcaacccga gactacccgt gaatgctcag tgtaaaatct cgtagccgg
//
LOCUS       DATADN010001046         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_270310, whole genome shotgun sequence.
ACCESSION   DATADN010001046 DATADN010000000
VERSION     DATADN010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270310"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..574
                     /locus_tag="VHH53_13445"
     CDS             266..574
                     /locus_tag="VHH53_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291166.1"
                     /translation="MTADGCRLRAAADENSDLFWALRGGGGNFGVVTEFEFRLYPVGP
                     IVHVGLLFWSLDHGAEALRFGWDVFATLPADANAMIISANAPPAPFVPQQYHFTPATG
                     "
     gene            613..1170
                     /locus_tag="VHH53_13450"
     CDS             613..1170
                     /locus_tag="VHH53_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX2291167.1"
                     /translation="MLAPIREALTPLFEFVTPMPYTQLQQMFDEAARWGVNCYEKALY
                     RDALPNEAIAVIIEHQLRKTSPQSMVHLFRLDGAYAEVDEAETAFGGSRSSRIAVNIV
                     AITDDVELLAAERTWVWSFWDALRPHATGAGGYINTMADIEEDRVRATYGLVKYERLA
                     RIKAKYDPDNVFHRNVNIKPALQPI"
     gene            1383..1586
                     /locus_tag="VHH53_13455"
     CDS             1383..1586
                     /locus_tag="VHH53_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX2291168.1"
                     /translation="MAQGVVKWFNAEKGFGFISVDGGGPDVFVHFSAIQGDGYRSLDE
                     NQRVEFESSQGPKGPQAESVRAI"
BASE COUNT          264 a          500 c          555 g          306 t
ORIGIN      
        1 ggtaccgcga tctgcgacgg cggcgtgatg atcgacctca gccagatacg cgacgttacg
       61 gttgaccctg caacgcgccg cgcgcgggtc ggcggtggcg caacgctggc cgatctggac
      121 gcagccaccc aggtacacgg cctggccgtg cctgctggca ctgtcagcca caccggcgtg
      181 ggcggtctcg cgctgggtgg cgggatgggg tggctgatcc cattgtacgg cctgactgca
      241 gacaacctcg tctccgctga ggtgggtgac cgccgacggg tgccgactgc gggccgccgc
      301 ggacgagaac tccgacctgt tctgggcact gcgaggcggc ggtggaaact tcggcgtcgt
      361 cacggagttc gagtttcggc tgtacccggt cggtccgata gtgcacgtcg gcttgttgtt
      421 ctggagcttg gaccacggtg cggaggcgct gcggttcggc tgggacgtgt tcgccacgtt
      481 gcccgccgat gcgaatgcca tgatcatcag cgccaacgct ccgccggcgc cgtttgtgcc
      541 tcagcagtac catttcaccc ctgctacggg ctagcggtgg tcggcttcgg ttccgtagag
      601 gagcatgccc acgtgctggc tccgatccgt gaggcgctta cgccgctgtt cgagttcgtc
      661 accccgatgc cctacaccca gctgcagcag atgttcgacg aagcagcacg gtggggcgtg
      721 aattgctatg agaaggcgct gtaccgcgac gccttgccca acgaggcaat tgcggtgatt
      781 atcgagcatc agctgcgtaa gacgtcgccg caatcgatgg tgcacctgtt ccgtctcgac
      841 ggtgcctacg cggaggtcga tgaggccgag accgcgttcg gcggcagccg ttcaagccgt
      901 atcgccgtca acatcgtcgc catcaccgat gacgtggagt tgctggcagc cgaacgtact
      961 tgggtctggt cgttctggga tgcgctgcga ccgcacgcca ccggtgccgg tggttacatc
     1021 aacaccatgg ccgacatcga ggaggatcgg gtgcgggcca cctatgggct ggtcaaatac
     1081 gagcggctgg ctcggatcaa ggccaagtac gatccggaca acgtcttcca ccgcaacgtc
     1141 aacatcaaac ccgcgttgca gccgatctag cgaatgtcgg gtgcaggtgg gcggaccggt
     1201 ggtgctagcc tgtcgccagg ttgtggtcgt gtagttcccc gatcgttgtg aagcgtccgt
     1261 ggaatgtctg actacgggct tcgcgctccc gtcggggatc tctcctatgg gtgcgcgtga
     1321 tccgcgcaac cgcgggccgg tacggtgccg gcccaggcat cagattccac agggagagca
     1381 acatggcaca gggcgtagtg aagtggttca acgcggagaa gggcttcggc ttcatctccg
     1441 tcgacggggg tggccccgac gtgttcgtcc acttctccgc catccagggt gacggctacc
     1501 gcagcctgga cgagaaccag cgcgtggagt tcgagtcaag ccagggtccc aagggcccgc
     1561 aggccgagtc ggtccgcgcc atctgagcgt ttcttacgcg gccccgtacc aagcctggtg
     1621 cgggg
//
LOCUS       DATADN010001047         6287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_113498, whole genome shotgun sequence.
ACCESSION   DATADN010001047 DATADN010000000
VERSION     DATADN010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113498"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VHH53_13460"
     CDS             <1..1882
                     /locus_tag="VHH53_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291169.1"
                     /translation="SDDSLHNVVSRGGSRVFFMAPDPVVAASANPGTTACSAATGEET
                     ACPPQLYVWQAGVGGGEPTVRWISRAEDGLFGLQDASLLGQAFFEGATPDGDKVFFRT
                     SSPLTMDDPNGVRDGLGNTVPPPPGGVVSGNDSLTSWDLYMYDFPDDPAADPGDGELT
                     RVSGGPAGAGDCNNPQGPTNAFTPVSNDRATSLRFVSDDGSRVLFVCSAPLPGVGPAS
                     NGTTTSPGGSPTAAFPAETNLYLYDTDTGAPRWRFVARLPRATATSAEARRLALCAST
                     GGYRGNPLAARANAVSFASSGVNCFDGLGDGGFVSFFTPGGLVAGDDAGSGDVYGYDM
                     DADVLVRLSEPEVGAAGGSYGCGGQQCFGDGGFDHSEWRHPLPLLSVVREPGGLGGRV
                     VLFQSRSRLVPEDLNDVYDVYSWRDGDLSLVTSGGAGADHVLYKGMDRSARNVYVATR
                     ENLSWQDVDRVGDIYVARVGGGFAQPPEPVLCAVLVGGCVGPGGGVAWGAAPVTATPG
                     GGGDAEPGARRRLSIGKVSRAARRRAARRGVLVLRVRSSGAGRVRLVARARVAGRRRV
                     VGRASKVLREPGSTRLRLRLRPVARRALGSGVRLRVRLVASQAGGALPRRATVRLRRP
                     GR"
     gene            1942..5316
                     /locus_tag="VHH53_13465"
     CDS             1942..5316
                     /locus_tag="VHH53_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291170.1"
                     /translation="MGQADRAGNRSLRVGGIVMAAVAAGALALALAVLAAGFAQRAEA
                     AFGDAYGLMPINDPASGSLVVPAIPGSDYLFWAGVCDRGAAPPAGSDLAALGGIGTMP
                     STATSPSDSLGVGSIVEVPLLGSVRDCIDWRLGLNGTTGSTDPENLWQPPPNWRLPEA
                     TRAGAHPDGTVTFGFVNDGDTDNIVVDLPPGVVGNPNAVPKCTADQFAVKPQQCPPST
                     QVGVLTLRHLGAGLGGANFGGNQNVETYPVFNLEPREGNLAELGLVQLTRDGRITARL
                     VAKARTGTDFGVTTFIGQLPGSALPITQQQITVWGVPWAPHNDVWRAPLGINGEGPCS
                     QQPGSGSPWHMIPPGGLPAGCRQSYDPSWGPVRPFFTTETDCNPAPITRLASDVYQRP
                     GPFTADGNPAIADYPSTGSDPAGNWKVYTTQSPPVTGCGSLPFSPQVGFTPTADAAGT
                     PQRSADSPAGLDVDLAVPQNNDPPAAVANDPAAADAHWRSDAGRATAHLKDTVVKLPW
                     GVAVNPSGATGLVGCEDAQMGVLDPNTSPMRFNDEDPFDDQGAECPDGSVIGTARVDT
                     PLLEEPLLGEVVLGQPKPQDVGGPSDPLTVRLFIVVKDPARGLVAKIYGRSQTDPATG
                     DLTARFENNPEVPFDRLQLSFRPGPRGLLRTSQRCGNHSWQSSFVPWSAAHGGGGQTV
                     ERQGAFEIQANCEFGFAPDLAAGMDNRAGGSFGGFSFEFSRNDGEQWIGGLTARLPEG
                     LLAAVRDVPLCTNAQAAANACPPGSRIGWVDAAAGAGDPFVLERKGDVYLTESYKGAP
                     YGLAVSVPVEAGPFRGPVALAPIVVRQQIQVDPNDASVTAVSDPLPTIWHGIPLAIRR
                     VLVKIDRNRFTINPTSCAPKQIGAIFNSNLATVTSATTPFQATNCRRLAFKPKLRMRL
                     TGRKQTKTGRHPGIRARVTQRRGEAAVKRAVVRLPKTLALDVRNAQALCEFDQGTRPD
                     LENHCPKGSIVGRARAVSPLLKRPLKGNVYFVKNIRIDKDTGNEIRTLPMIIAALRGE
                     VAVNLRGNSSVGKGGKLISTFANVPDAPIRRFNLNIRGGKTGIIAVTRKRRAGAINIC
                     NSRQIAVTNFDGQNARRHDRNLKIKTPPCNKPKKRR"
     gene            5553..6233
                     /locus_tag="VHH53_13470"
     CDS             5553..6233
                     /locus_tag="VHH53_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291171.1"
                     /translation="MRNLRKLVLLATTAIAALALPTTTATAQHPGYEHPEGTVELTNE
                     PTGEHCTDFTVEGHQPVGANCTLHATGSFSFFIHTGLSEAQFTACQNEFEAAFNENGE
                     GVIYDQILAPEAGTCGREVCDEAGAGGENHPNIPWGAELFEAPDDGGLKLGYRFCLYA
                     HSDGDEGQVGTVCHIILDVAHVEHAYELSTPPTGALCEELPIEIVGHWVTGLTEEHPD
                     SIETIHLD"
BASE COUNT          992 a         2043 c         2208 g         1044 t
ORIGIN      
        1 gagcgacgac tcgttgcata acgtggtgtc gcggggtggg tcgcgggtgt tcttcatggc
       61 gccggatccg gtggtggcgg cgtcggcgaa tccggggacg actgcgtgta gcgcggcgac
      121 gggtgaggag acggcgtgtc cgccgcagtt gtatgtgtgg caggcgggtg tgggtggtgg
      181 ggagccgacg gtgcggtgga tctctcgggc tgaggatggg ttgtttggtt tgcaggatgc
      241 gtcgttgttg ggccaggcgt tttttgaggg cgcgacgccg gatggcgaca aggtgttctt
      301 tcggacgagc tcgccgttga cgatggacga tcccaatggt gtgcgggatg ggttgggcaa
      361 cacggttccg ccgccgccgg gtggggtggt gtctggcaat gacagcttga cgtcgtggga
      421 tctgtatatg tatgactttc ccgacgaccc ggctgcggat ccgggcgatg gggagctgac
      481 gcgggtgtcg ggcggcccgg ccggcgcggg ggattgcaac aacccgcagg gccctacgaa
      541 cgcattcacg ccggtgtcga acgatcgggc gacgtcgctg cggttcgtgt ctgacgacgg
      601 gtcgcgggtg ttgttcgttt gttcggcgcc gttgccgggg gttgggccgg cgtcgaatgg
      661 gacgaccacc tcgccggggg gcagtccgac ggcggcgttt ccggccgaga cgaatctgta
      721 tctctatgac accgatacgg gcgcgccgcg gtggcggttc gtagcgcggc tgccgcgcgc
      781 gaccgctacg tctgcggagg cgaggcggct tgcgctctgt gcgagcacgg gcggctatcg
      841 ggggaatccg ttggccgcgc gcgccaatgc ggtgtcgttt gcctcgagcg gtgtgaattg
      901 ctttgatggt ttgggtgatg gtgggtttgt gtcgttcttt acgccgggcg gtcttgtggc
      961 tggggatgat gccgggtcgg gtgatgtgta tggctatgac atggacgcgg atgtgttggt
     1021 gcggttgtct gagcctgagg ttggcgcggc tggggggtcg tatgggtgtg gtggccagca
     1081 gtgctttggc gacggcggct tcgatcattc ggagtggcgg catccgttgc cgttgttgag
     1141 tgtggtgcgc gagccgggtg gtttgggcgg tcgggttgtg ttgttccagt cgcgcagccg
     1201 gctcgtgccc gaggatctga acgatgtcta tgacgtgtat tcgtggcgtg atggcgattt
     1261 gtcgcttgtg acctcgggtg gggcgggtgc tgatcatgtg ctgtataagg gcatggatcg
     1321 ctcggcgcgg aatgtgtatg tcgccacacg ggagaacttg tcgtggcagg acgtcgatcg
     1381 ggtgggcgat atctatgtcg cgcgtgtcgg cggtgggttt gcccagccac cggagcctgt
     1441 gctgtgcgct gtgctggtcg gtgggtgtgt tggtccgggt ggcggtgtgg cgtggggcgc
     1501 ggcgcccgtt accgctaccc cgggcggtgg cggtgacgcc gagccgggcg cgcggcggcg
     1561 gctttcgatc ggcaaggtct ccagggcggc gcgccggcgg gctgccaggc gtggggtgtt
     1621 ggtgttgcgg gttcggtcca gcggggctgg gcgtgtgcgg ctggtcgcgc gcgccagggt
     1681 cgcaggccgg cggcgggtcg tgggccgggc gtcgaaggtc ttgagggagc cggggtcgac
     1741 gcggctgcgg ctgcggttga ggcctgtggc gcggcgggcg ttgggctcgg gtgtccggtt
     1801 gcgggtcagg ctggtcgcct ctcaggcggg cggcgcgttg ccgcgcaggg cgacggtgcg
     1861 gttgcggagg ccgggccggt gaggcgcgga cggcgagggt ttgggatcgg caacgggttc
     1921 atgagcgtta gggagatcgt ggtgggtcaa gcagacaggg ccggtaacag gagcctgcgg
     1981 gtcggcggaa tcgtgatggc ggcggtcgcg gcgggcgcgt tggctttggc gctggcggtt
     2041 ttggcggcgg ggtttgcgca gcgtgccgag gcggcgtttg gggatgccta tgggctgatg
     2101 ccgatcaacg atccggcgtc ggggtcgttg gtggttcccg cgatcccggg ttcggattac
     2161 ctgttttggg cgggtgtttg cgatcgcggc gcggcgccgc cggcgggcag cgaccttgcg
     2221 gcgcttggcg ggatcgggac gatgccctcc acggcgacct ctcccagcga cagcttgggg
     2281 gtcggctcga tcgtcgaggt gccgttgctt ggcagcgtgc gcgattgcat cgattggcgg
     2341 ctgggcctga acgggaccac cgggtcgacc gatcctgaga atctttggca gccgccgccg
     2401 aactggcgtt tgccggaggc gacgcgggcg ggcgcgcatc ccgacggcac cgtgacgttc
     2461 ggctttgtca acgatggcga taccgacaac atcgtggtcg atctgccgcc gggggtggtc
     2521 ggcaatccaa acgcggtgcc caagtgcacc gccgatcagt tcgcggtcaa gccgcagcag
     2581 tgcccgccgt ctacccaggt gggtgtgttg acgttgcgcc acctcggcgc tgggcttggc
     2641 ggcgcgaact tcggcggcaa ccagaacgtc gagacctatc cggtgttcaa cctcgagccg
     2701 cgcgagggaa acctcgccga gctggggttg gtgcagctga cccgcgacgg gcggatcacc
     2761 gcccggctgg tcgctaaggc ccgcaccggc actgatttcg gcgtcacgac cttcatcggc
     2821 cagctgccgg ggtcggcgtt gccgatcacc cagcagcaga tcaccgtttg gggggtgccg
     2881 tgggcgcctc acaacgacgt ctggcgcgcc ccgcttggca tcaacggcga ggggccgtgc
     2941 tcccagcagc cggggtcggg ctcgccgtgg cacatgatcc cgccgggtgg cctgccggct
     3001 gggtgtcggc agagctatga cccgtcgtgg ggtccggtcc gtccgttctt cactaccgag
     3061 accgattgca accccgcgcc gatcacgcgc ctggcctctg acgtctacca gcgccccggc
     3121 cccttcaccg ccgacggcaa ccccgcgatc gctgactatc cgtccacggg gtcagacccg
     3181 gcgggcaact ggaaggtcta caccacccag tcgccgccgg tgaccggttg tgggtcgctg
     3241 ccgttctcgc cgcaggtcgg cttcaccccg accgccgacg cggccgggac gccgcagcgc
     3301 tcagccgaca gccccgcggg cctggacgtc gatctggcgg tgccccagaa caacgacccg
     3361 cccgcagctg tcgccaacga tccggcggcc gcggatgcgc actggcgctc tgacgccggc
     3421 cgcgctaccg cgcatctcaa ggacaccgtg gtcaagctgc cgtggggtgt ggctgtcaac
     3481 ccctcgggcg cgacgggcct ggtcggctgc gaggacgccc agatgggagt cctcgatccc
     3541 aacaccagcc cgatgcgctt caacgacgag gatcccttcg acgaccaggg cgccgagtgc
     3601 ccggacgggt cggtgatcgg cacagcacgc gtcgacaccc ccttgctcga ggagccgctt
     3661 ctgggcgagg tggtgttggg tcagccaaag ccccaggacg tcggcgggcc cagcgacccg
     3721 ttgaccgtcc ggctgttcat cgtcgtcaaa gaccccgccc gcgggctggt cgcaaagatc
     3781 tatggccgct cccagacaga ccccgcgacc ggcgacctca ccgcacggtt tgagaacaac
     3841 cccgaggttc ccttcgaccg gctgcagctg tcgtttaggc ccgggccgcg aggacttttg
     3901 cggacctctc agcgctgcgg caaccacagc tggcagagct cgttcgtgcc gtggtcggcc
     3961 gctcatggcg gcggcgggca gaccgtcgag cgccagggcg cgtttgagat ccaggccaac
     4021 tgcgagttcg gcttcgcgcc cgatctggcc gcggggatgg acaaccgcgc cggcggcagc
     4081 ttcggcgggt tctccttcga gttctcgcgc aacgacggcg agcagtggat cggcgggctg
     4141 accgccaggc tgcccgaggg gctgctcgcc gctgtccgag acgtgccgct gtgcaccaac
     4201 gcccaagccg ccgccaacgc ctgcccgccc ggatcgcgga tcgggtgggt cgacgccgca
     4261 gccggcgcgg gcgacccgtt cgtgctcgag cgaaagggcg acgtctacct cacagagagc
     4321 tacaagggcg ccccctacgg cctcgcggtc tcggtcccgg tcgaggccgg cccgttccgc
     4381 ggcccggtcg cgctcgcgcc gatcgtcgtg cgccaacaga tccaagtcga cccaaacgac
     4441 gcgtcggtca ccgcggtctc cgacccgctg ccgacgatct ggcacgggat tccgctggcg
     4501 atccgccgcg tgctggtcaa gatcgaccga aaccggttca ccatcaaccc gacaagctgc
     4561 gccccgaagc agatcggcgc gatcttcaac tccaacctcg ctaccgtcac ctccgccacc
     4621 acacccttcc aagccaccaa ctgccgccgg cttgcgttta agccaaagct caggatgcgc
     4681 ctgaccggcc gcaagcagac aaagaccggc cgccaccccg gcatccgcgc ccgcgtcacc
     4741 caacgccgcg gcgaggcagc cgtcaaacga gcggtggtcc gcctaccaaa gaccctcgcg
     4801 ctcgacgtcc gcaacgccca agcgctctgc gagtttgacc aaggcacccg cccagacctc
     4861 gagaaccact gccccaaggg ctcgatcgtc ggccgcgccc gcgccgtctc cccactactc
     4921 aaacgcccac tcaaaggcaa cgtctacttc gtcaagaaca tccgcatcga caaagacacc
     4981 ggcaacgaga tccgcaccct gccgatgatc atcgccgccc tacgcggcga ggtcgcggtc
     5041 aacctacgcg gcaactccag cgtcggcaag ggcggcaagc tcatctcgac cttcgccaac
     5101 gtccccgacg ccccaatccg acgcttcaac ctcaacatcc gaggcggcaa gaccggcatc
     5161 atcgccgtca ccagaaaacg acgggcaggc gcgatcaaca tctgcaacag ccgccaaatc
     5221 gccgtcacca acttcgacgg ccaaaacgcc cgccgccacg accgcaacct caaaatcaaa
     5281 accccaccct gcaacaaacc caaaaaacga cgctaacccc ccctcccccg cggcgaagcg
     5341 tcccccgcag ggattgacaa ccctctaagt tccggtatcg tcgcacccac tgccgtgtcc
     5401 aatgcgacac ggagtgatgt cccccacacg gagggagggg gcccgcagcg accaaaacac
     5461 cagccgccaa caacggcagc ccactgcggc aaaccaagcg cgccaaaccc ggcgcccgcc
     5521 acggtgccgg acacccatgg agggaaagaa tcatgcgcaa cctgcgcaag ctcgtgctgc
     5581 tcgccaccac ggccatcgca gcactcgcac tcccaacaac aaccgccacc gcccaacacc
     5641 ccggctacga gcaccccgag ggcaccgtcg aactgaccaa cgaacccaca ggagagcact
     5701 gcaccgactt caccgtcgag ggtcatcagc ccgtcggagc gaactgcaca ctccacgcca
     5761 caggcagctt tagcttcttc atacacaccg gtctctctga agcgcagttc acggcctgcc
     5821 agaacgaatt cgaagccgcc ttcaacgaaa acggcgaggg ggtcatctac gaccagatac
     5881 tagctcccga agcgggcacg tgcggccgcg aggtttgcga tgaagcggga gcgggtggtg
     5941 aaaatcaccc caacattccg tggggagcgg aactgttcga agcccccgac gatggcggcc
     6001 tcaagctcgg atacagattc tgcctatacg cccacagtga tggcgacgaa ggccaggtcg
     6061 gcaccgtttg ccacatcatc cttgacgtgg cgcacgtgga gcacgcctac gaactcagca
     6121 cacccccgac gggcgctctc tgcgaagaac tcccaatcga aatcgtaggc cattgggtga
     6181 ccgggctcac cgaggagcat ccagacagca tcgaaaccat ccatctcgac tagggcagcc
     6241 tgccctggag accatgcgct ccgggcgcag gaaccgagga gtggggg
//
LOCUS       DATADN010001048         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_585543, whole genome shotgun sequence.
ACCESSION   DATADN010001048 DATADN010000000
VERSION     DATADN010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585543"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VHH53_13475"
     CDS             complement(<1..377)
                     /locus_tag="VHH53_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX2291172.1"
                     /translation="MGLRVNNNIAALNAYRNLSATDNQLSKSLEKLSSGFRINRAADD
                     AAGLAISEGLRSQIGGLKVAVRNTQDGISVVQTAEGALTETHSILQRMRDLSVQASNA
                     GGLNDVAKGNIQSEIVQLKSELDR"
     gene            495..1694
                     /locus_tag="VHH53_13480"
     CDS             495..1694
                     /locus_tag="VHH53_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX2291173.1"
                     /translation="MVNSPTRHSGGLRGSGGAAGLAISEGLRSQIGGLKVAVRNTQDG
                     ISVVQTAEGALTETHSILQRMRDLSVQAANSGGLNDDAKANLQSEIVQLKAELDRIAN
                     TTNFNGKKLLDGSFTSTFQVGANSGETISVNIATAMGSAGLGVDGVDVTDVGAYTFGA
                     AAAAGVANISTAAADGTGAVMTITTKDAANEAPNADLEFFTRLTGTISFAGKSLDLGS
                     IDYTNADGDNDPTTVPTAADALAVLNQAATDLWGLATDPFAVTATTLTFAVQDAVSGY
                     TEAGGDPVGSAAGTPTAAQLSAATPTFTAASGASDAIDVLDDAINKVSTQRADLGAIQ
                     NRFDHTINNLNVAVENLTASESRIRDADMAQEMVQFTRNQILSQAGTAMLAQANSASQ
                     GILQLLR"
BASE COUNT          287 a          635 c          592 g          263 t
ORIGIN      
        1 cggtcgagct cggacttcag ctgcacgatc tccgactgga tgttgccctt ggcgacgtcg
       61 ttgaggccac cggcgttgga ggcctgcacc gacaggtcgc gcatgcgctg caggatgctg
      121 tgcgtctcgg tgagcgcacc ctcagcggtc tgcacgaccg agatgccgtc ctgggtgttg
      181 cggacggcga ccttgaggcc accgatctga gagcgcaggc cctcggagat ggccagaccg
      241 gccgcgtcgt cggcggcacg gttgatgcgg aagccggagg agagcttctc cagcgacttg
      301 ctcagctggt tgtcggtcgc cgacaggttg cggtacgcgt tgagcgccgc gatgttgttg
      361 ttgacgcgca gacccattgt gttcctcctt ggacggggtc tctgtggtga tcccgctgca
      421 tccgtgctgc gggagccgtg ccggtcctgc ctcagcagtt acggccgcgg ccatcgggga
      481 cttgaggcga aaccgtggtc aattcgccca cccgtcactc aggcgggttg cgcggctccg
      541 gcggcgcggc cgggctcgcc atctcggagg gcctgcgctc gcagatcggt ggtctgaagg
      601 tcgccgtccg caacacccag gacggcatct cggtcgtgca gaccgctgag ggtgcgctca
      661 ccgagacgca cagcatcctg cagcgcatgc gcgacctgtc ggtgcaggcc gccaactccg
      721 gtggcctcaa cgacgacgcc aaggccaacc tgcagtccga gatcgtgcag ctgaaggcgg
      781 agctggaccg gatcgccaac accacgaact tcaacggcaa gaagctgctc gacggcagct
      841 tcacctccac cttccaggtg ggcgccaaca gcggcgagac gatctcggtc aacatcgcca
      901 cggcgatggg ctcggccggg ctgggggtcg acggggtcga cgtcaccgac gtcggtgcct
      961 acaccttcgg cgccgctgcg gcggccggcg tggcgaacat cagcacggcg gccgccgacg
     1021 gcaccggtgc ggtcatgacc atcaccacca aggacgcggc caacgaggcg ccgaacgccg
     1081 acctggagtt cttcacccgg ctgaccggca cgatctcctt cgccggcaag tcgctcgacc
     1141 tcggctcgat cgactacacc aacgccgacg gtgacaacga cccgaccacc gtgccgaccg
     1201 ccgccgacgc cctggcggtg ctcaaccagg cggccacgga cctgtggggg ctggccaccg
     1261 acccgttcgc ggtgaccgcc acgacgctga ccttcgccgt ccaggatgcg gtcagcggct
     1321 acacggaggc cggcggtgac ccggtcggct cggcggcggg caccccgacc gcggcccagc
     1381 tgtcggcggc caccccgacg ttcaccgcgg ccagcggcgc gtcggacgcg atcgacgtgc
     1441 tggacgacgc catcaacaag gtgtcgaccc agcgtgccga cctgggtgcg atccagaacc
     1501 ggttcgacca cacgatcaac aacctcaacg tggcggtgga gaacctgacc gcctcggaga
     1561 gccgcatccg cgacgccgac atggcccagg agatggtgca gttcacccgc aaccagatcc
     1621 tgtcccaggc cggcaccgcg atgctcgccc aggccaactc ggcctcgcag ggcatcctcc
     1681 agctgctccg ctgacctccg gtcagctgat cggctgaggc gcaccacctc agctccaccc
     1741 cacgctgaag gggggaggcc tccgggcctc ccccctt
//
LOCUS       DATADN010001049         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_731937, whole genome shotgun sequence.
ACCESSION   DATADN010001049 DATADN010000000
VERSION     DATADN010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731937"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1569
                     /locus_tag="VHH53_13485"
     CDS             175..1569
                     /locus_tag="VHH53_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX2291174.1"
                     /translation="MHGLPRAERDGLGGGHRGSVDRAGVDVDAARHVALLVGAGNNGG
                     DALWAGAFLRRRAVAVTAVLLSPDRVHPQGLAALRRAGGRIDDRLPDRADLILDGIVG
                     LSGRGGLRPAAAQLVQGAQVPIIAVDSPSGVDPDTGIADGPAVTAAETVTFGVRKPVH
                     LLTPWRCGPVHLVDIGLFGELGEPDLRVLTAAEIGARWPVPGPSSDKYNQGVLGIAAG
                     SADYPGAAVLCAGAAVLATSGMVRFAGTAADAVRARWPEVVATGSVSDAGQVQAWAVG
                     PGLGTGTAGHQVLAHVLAAGVPVVADADAITLLARHPDLADSRDPDTPLVVTPHDREF
                     ERLTGKAPGPDRVGAVRTTACRFNAVLVLKGNTTIIAAPDGRVLVNPARSSWPATAGS
                     GDVLTGVLGALLAAGLEPATAAAAAVHAQEFAAAIAAGGGTHSAGAPAPASAIQAALS
                     DAIRALRWMAGMRG"
     gene            1566..2729
                     /gene="alr"
                     /locus_tag="VHH53_13490"
     CDS             1566..2729
                     /gene="alr"
                     /locus_tag="VHH53_13490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2291175.1"
                     /translation="MTTMQVEGSRPRAELVIDLDAIRHNVGILAGCAAASGATTMAVV
                     KADGYGHGAVDVAVAALQAGAGALGVCSVDEALELRHSGIDAPVLAWLHAPGEDLAAG
                     IAAEVDLGIYSTGQLDTATAAAAAIGRTARVHLKVDTGLTRGGACRGEWPGLVRAAAQ
                     TPGVDVVGVWSHLAHADDPGHPIIEQQVRYFDEAYQVARDAGLRPLRHLANSAATLTR
                     PDLHYDMVRPGIAVYGLSPVPEMSYHLLPAMTLRSQVAMIKRVPAGEGVSYGHVWHTE
                     RETTLALVPAGYADGVPRVLTGRLDVWLAGRRRPVVGRVCMDQVMVDCGDDPVTEGAE
                     VIFFGTGENGAPTAVEWADKLDTIHYEVVAGMVRPRLTRTVRGRHPTLTRVVR"
BASE COUNT          361 a          949 c         1033 g          432 t
ORIGIN      
        1 tccggcggcg acgcccgtca ccccctgtgt gtacttgtcg tccgccgggc cgggcaccgg
       61 ccagcgggcc gcgacgtcgt ccgcctcgag aacgtgcgcg tgcggggccg gcaggtgcgg
      121 ccccagcccc aggtcgacca ggtgcacccg cccgcagcgg gacgcggcca gcacgtgcac
      181 gggcttccgc gcgccgaacg tgacggtctc ggtggcggtc accgcgggtc cgtcgaccgc
      241 gccggtgtcg acgtcgacgc cgctcggcac gtcgccctgc tggtcggggc gggcaacaac
      301 ggcggggatg cgctgtgggc cggggcattc ctgcgccgcc gcgcagtggc ggtcaccgcc
      361 gtgctgctgt ccccggaccg cgtccatccg cagggtctcg cagcgctgcg gcgagccggg
      421 ggacggatcg atgaccggct gccagaccgc gctgacctca tactcgacgg gatcgtcggg
      481 ctgtccggcc gtggcgggtt gcgcccggcg gccgcgcagc tggtccaagg cgcccaggtg
      541 ccgatcatcg cggtggactc gccgtccggg gtggacccgg acaccggcat cgcggacggg
      601 cccgcggtga ccgccgccga gaccgtcacg ttcggcgtcc gcaagccggt gcacctgctc
      661 acgccgtggc gctgcggccc cgtgcacctg gtggacatcg gcctgttcgg tgagctgggc
      721 gaacccgatc tgcgggtgct caccgcagcc gagatcggtg ctcgctggcc ggtacccggc
      781 ccgtccagtg acaagtacaa ccagggcgtg ctgggcatcg cggcgggttc ggcggactac
      841 cccggagctg cggtgctctg cgccggggcc gccgtactcg cgacatcggg catggtgcgc
      901 ttcgccggca ccgccgccga tgcggtacgc gcgcgctggc cggaggtggt ggccaccggc
      961 tcggtgtccg acgccggtca ggtgcaggcc tgggccgtcg ggcccggctt gggcaccggc
     1021 accgcaggtc accaggtgct cgcccacgtg cttgccgcgg gagtgccggt agtggccgac
     1081 gccgatgcga tcaccctgct agcccgccac cccgatctgg cggacagtcg cgatccggac
     1141 acgccgctgg ttgtcactcc gcacgaccgg gaattcgagc ggctgactgg caaggcaccc
     1201 ggtcccgacc gggtcggtgc cgtccgaacc accgcctgcc ggttcaatgc tgtgctggtg
     1261 ctcaagggaa acacgaccat catcgctgct ccggacggcc gggtgctagt gaaccccgct
     1321 cgctcgtcgt ggccggccac cgcaggttcc ggtgacgtcc tcaccggtgt gctcggcgcg
     1381 ttgctggctg ccgggttgga accagccacg gcggcggccg ccgcagtgca cgcccaggag
     1441 ttcgcagctg ccattgccgc tggtggcggg acgcacagcg ccggcgcacc ggcgcctgcc
     1501 tcggcgatcc aggcagctct gagtgacgcg attcgcgctc tgcggtggat ggctgggatg
     1561 cgaggatgac cacgatgcag gttgagggca gccggccgcg ggccgagttg gtgatcgatc
     1621 tggatgcgat ccggcacaac gtcggaatcc tcgccgggtg tgccgctgcg tccggggcga
     1681 ccactatggc agtggtcaag gccgacggct atggccacgg agcggtggac gttgccgtcg
     1741 ccgcgctgca ggcgggggcc ggcgcgctag gcgtatgcag cgtcgacgag gcgctcgagt
     1801 tacgtcacag cggaatcgac gctcctgtgc tggcctggct gcacgctccc ggtgaggacc
     1861 tcgctgctgg aattgccgct gaggtcgatc tcggcatcta ctccaccggt cagctcgaca
     1921 ccgcaactgc agctgccgca gcgatcgggc gcaccgcccg ggtacacctc aaggtcgaca
     1981 ccgggttgac ccgaggcggc gcctgccgtg gcgaatggcc cggcctggtc cgtgccgctg
     2041 cgcagacccc gggcgttgac gttgtcgggg tctggtcgca ccttgcgcat gccgacgatc
     2101 cgggacaccc gatcatcgag cagcaggtcc ggtacttcga tgaggcctac caggtggctc
     2161 gggatgccgg gctgcggccg ctgcggcatc tggcgaactc cgcggcgacg ttgacccgcc
     2221 ccgatctgca ctacgacatg gtccgcccgg gtatcgccgt ctacgggctc tccccggtgc
     2281 ccgagatgag ttaccatctt ttgccagcga tgacactgcg ctcgcaggta gcgatgatta
     2341 aacgcgtccc ggctggcgag ggtgtgtcat acgggcacgt ctggcatacc gaacgggaaa
     2401 ccaccctcgc attggttccc gccggttacg ccgacggcgt gccgagggtg ctcaccggtc
     2461 ggcttgacgt ctggctggcg gggcgccgtc gcccggtggt gggaagggtg tgcatggacc
     2521 aggtgatggt cgactgcggg gatgacccgg tgaccgaggg cgctgaggtg atcttttttg
     2581 ggaccggcga gaacggcgca cccaccgcgg tcgagtgggc ggacaagctg gacaccatcc
     2641 actacgaggt ggtcgcgggg atggtgcgcc ctcggttgac ccggacagtg cgcggccgcc
     2701 atccgactct gacgcgagtc gtccgatgag gtcggcggtg aagtctgccg ggtgggtggc
     2761 cggcggtgtg ctggg
//
LOCUS       DATADN010001050         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_871747, whole genome shotgun sequence.
ACCESSION   DATADN010001050 DATADN010000000
VERSION     DATADN010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871747"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2018)
                     /locus_tag="VHH53_13495"
     CDS             complement(<1..>2018)
                     /locus_tag="VHH53_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HEX2291176.1"
                     /translation="FPNAGNWAQDPNDPNGYLSHQPGVVWVTFDPRTGTRGNATQTIY
                     VGVADLNNTVYRTTNGGTTWERVAGQPTGLMAQKSVLDPVNGFLFITTSNNGGPYDGT
                     SGAVWRYTTATGAWTNVTPPPPLGNAQPYYGHAGLTIDRQRPGTVMVTGYSSWWPDTW
                     IFRSTDSGATWQRFWDVPSFPTRVNRYTQDITAVPWLSFGPNTNPQPPETSPKLGWMT
                     QAMAIDPFNSNRFMYGTGATIYGSTNLTTFDTGGTVTIRPMVAGLEETAVLDLISPPS
                     GPPLVSGLGDIGGFRHNNLDAVPARTFTAPYHTTTTSLDYAETNPSVMVRAGNFADSE
                     RPNDSHVAFSTDGGANWFQGTEPGGINEGGTVAAAANGSRFVWAPRGQTPVFSVGFGN
                     SWTQAQGLPTDSIVESDRVNPNKFYGFNGGRFYVSTNGGQSFTATVQTGLPSTAKFKA
                     VPGREGDIWLAGDTGLFRSTNSGASFTRVSNVTSARNVGFGRAAPNQTYPALYAVATV
                     DGVTGVFRSDNAAAAWVRVNDDAHQYGNMGEALTGDPRVYGRVYLGTNGRGILYADRT
                     GAPPTSSTTTTTTTTTTTTTTSAPPPAGGCRASYRTEQWPGGFNAHVTITNTGTAAID
                     GWTLRFAFTAGQRITNGWSATWSQSGSTVSATNLTWNAAIAPGGSVEIG"
BASE COUNT          254 a          646 c          761 g          357 t
ORIGIN      
        1 ccgatctcca ccgagccgcc gggcgcgatc gcggcgttcc acgtcaggtt ggtcgcggac
       61 acggtggacc cgctctgcga ccaggtggcg ctccagccgt tggtgatccg ctgccccgcg
      121 gtgaaggcga accgcagcgt ccagccgtcg atggcggccg tgccggtgtt ggtgatcgtg
      181 acgtgggcgt tgaagccacc gggccactgc tcggtccggt agctcgcccg gcacccgccc
      241 gccggaggcg gcgcgctggt cgtggtcgtg gttgtagtgg ttgtcgtggt tgtcgtagtc
      301 gacgacgtcg gtggcgcgcc ggtgcggtcg gcgtagagga tgccgcggcc gttggtgccc
      361 aggtacaccc ggccgtacac ccgcgggtcg ccggtgagcg cctcgcccat gttgccgtac
      421 tggtgcgcgt cgtcgttgac ccgcacccag gccgcggcgg cgttgtccga ccggaacacc
      481 ccggtcacac cgtccacggt ggccaccgcg tacagcgccg ggtaggtctg gttcggagcc
      541 gctcggccga agccgacgtt acgtgcggat gtcacgttcg acacacgagt gaaactagcg
      601 cccgagttgg tggaacggaa cagtcccgtg tcaccggcaa gccagatgtc gccctcccgg
      661 ccgggcaccg ccttgaactt ggccgtgctg ggcagcccgg tctgcacggt ggcggtgaag
      721 ctctggccgc cgttggtgct cacgtagaac cggccgccgt tgaagccgta gaacttgttg
      781 gggttcaccc ggtccgactc gacgatcgag tcggtcggca ggccctgcgc ctgggtccag
      841 gagttgccga agccgaccga gaacaccggg gtctggccgc gcggcgccca gacgaaccgg
      901 ctgccgttgg ctgcggcggc gaccgtgccg ccctcgttga tcccacccgg ttcggtgccc
      961 tggaaccagt tcgcgccgcc atcggtggag aacgccacgt ggctgtcgtt gggtcgctcg
     1021 ctgtcggcga agttgccggc gcgcaccatc acgctcgggt tggtctcggc gtagtcgagg
     1081 ctggtggtcg tggtgtggta cggcgcggtg aacgtccggg ccggtacggc gtcgaggttg
     1141 ttgtgccgga atccaccgat gtcaccgagc ccggacacca gtggcgggcc ggacggcgga
     1201 ctgatcaggt cgagcaccgc ggtctcctcg aggccggcga ccatcggccg gatcgtcacg
     1261 gttccgccgg tgtcgaacgt ggtcaggttg gtgctgccgt agatcgtggc gccggtgccg
     1321 tacatgaacc ggttggagtt gaacgggtcg atcgccatcg cctgggtcat ccagcccagc
     1381 ttcggcgacg tctccggcgg ctgcgggttg gtgttggggc cgaaggacag ccacggcacc
     1441 gcggtgatgt cctgcgtgta gcggttgacc cgggtgggga agctcggcac gtcccagaac
     1501 cgctgccagg tggccccgct gtcggtgctg cggaagatcc aggtgtccgg ccaccacgag
     1561 ctgtagccgg tgaccatgac cgtgcccggc cgctgccggt cgatggtcag cccggcgtgc
     1621 ccgtagtacg gttgggcgtt gccgagcggc ggcggcgggg tgacgttggt ccacgcgccg
     1681 gtggcggtgg tgtagcgcca gaccgccccg gaggtgccgt cgtacgggcc gccgttgtta
     1741 ctggtcgtga tgaacaggaa tccgttgacc gggtcgagca cgctcttctg ggccatgaga
     1801 ccggtgggct gcccggcgac gcgctcccag gtcgtgccgc cgttggtggt gcggtacacg
     1861 gtgttgttca ggtcggcgac gccgacgtag atggtctgcg tcgcgttgcc tctggtgccg
     1921 gtgcgcggat cgaacgtcac ccacaccacg ccgggctggt gggacaggta gccgttgggg
     1981 tcgttcgggt cctgcgccca gttgccggcg ttcgggaa
//
LOCUS       DATADN010001051         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1323547, whole genome shotgun sequence.
ACCESSION   DATADN010001051 DATADN010000000
VERSION     DATADN010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323547"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VHH53_13500"
     CDS             <1..752
                     /locus_tag="VHH53_13500"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2291177.1"
                     /translation="QIGIERSAYAQLGAVPIIPGACAAWRKAAVAEVGGYSNATLAED
                     CDLTLSLHQAHWRVSQDDEALAYTEAPDHADALLAQRIRWTFGTLQAIFKHRNMLLRR
                     RYGWLGMAVLPYMAVSVLVPIVFLPFIAVMGVLAVQNSGWGTVGLYFLLFLVMHLLIA
                     AVAVVLMRERWANLLMVPIYRVVHEPLRAYLLYTSAYLAIRGVKLGWNKLQRTGVMDA
                     VVDVHGLRHSDPIPVERQSNAVRTLEEASTT"
     gene            749..>2125
                     /locus_tag="VHH53_13505"
     CDS             749..>2125
                     /locus_tag="VHH53_13505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2291178.1"
                     /translation="MTTSISSTVPPLEGPDHRAVGTVAEQPSSSRPPQDDGAGPRRDR
                     YIDTLRAGALLRVIIYHTFGFAWLPFLFPSMGIMFALAGGLVAASLDRAQHNPWKVLR
                     KRARRLLPPLWMLGIVLVPIMIVHGWVAVADEGGVPLDGWALLHWVVPLGDPGASDWG
                     YQFTIPLWYIRAYFWFLLLSPAALFMFRRWPKRTMSVPLIVAVLSGLGVFDLSLQGST
                     SSAIVATMATFGACWMIGFAHHDGTLRNLSLRWVIPASLASIAIGTWWAATRASWALD
                     DDTLGQALYCLGFVLLLLRFYPSFQWLQRRPLLDKLIAVVNSRAMTIYLWGNVAIFAA
                     GVLISSGWMPRIVTSNDVTAGLSRLVLALVVIALAVLAVGWVEDVAAHRTPRLNPWPR
                     TLPEHGAMTSSGLLRPADGSPQVHLRLPRRRLAGVLLMAGLAATLTLLWTAGDRSGVA
                     PRTTEQGQT"
BASE COUNT          301 a          745 c          681 g          398 t
ORIGIN      
        1 cccagatcgg catcgagcgc tcggcatacg cccagctagg agcggtgccc atcatccccg
       61 gggcctgcgc ggcctggcgc aaggcggcgg tggcagaggt cggcggctac tccaacgcga
      121 cattggccga ggactgcgac ctgacgctgt cgctgcacca ggcgcactgg cgggtcagcc
      181 aggacgacga ggcgctggcc tacaccgagg cccccgacca tgccgacgcc ttgctggcgc
      241 agcgcatccg ttggaccttc ggcacgctgc aggccatctt caagcaccgc aacatgctgc
      301 tgcgccgccg gtacggatgg ctcgggatgg ccgtgctgcc ctacatggcg gtctcagtcc
      361 tggtgcccat cgtgttcctg ccgttcatcg cggtgatggg cgtcttggcg gtgcagaact
      421 ccggctgggg caccgtcggt ctctacttcc tgctgttcct cgtgatgcat ctgctcatcg
      481 cagccgtcgc ggtggttctg atgcgagagc ggtgggcgaa tctgctcatg gttccgatct
      541 accgcgtggt gcacgagccg ctgcgcgcat acctgctgta cacgtccgcc tacctggcca
      601 ttcgcggcgt caagctcggg tggaacaagc tccagcgcac cggcgtcatg gacgccgtcg
      661 tcgacgtgca cggcctgcgc cacagcgacc ccattcccgt ggagcggcag tcgaacgccg
      721 tccgaacctt agaggaagca tcgacaacgt gacgaccagc atcagctcga cggtccctcc
      781 gctggagggt ccggaccacc gggccgtggg cactgtcgcg gaacagccat catcgtctcg
      841 gccgcctcag gacgacggcg cgggtccgcg tcgtgaccgc tacatcgaca cgctgcgggc
      901 cggcgccctg ctccgggtca tcatctacca caccttcgga ttcgcctggc tgccgttcct
      961 cttcccctcg atggggatca tgttcgcgct tgccggaggg ctggtcgcgg catcgctgga
     1021 ccgggcacaa cacaatccgt ggaaggtcct gcgaaaacga gcgcggcgcc ttctcccgcc
     1081 actgtggatg ctcggcatcg tcctggtccc gatcatgatc gtccacggct gggtcgctgt
     1141 ggccgatgag ggcggagttc cgctcgacgg gtgggcactg ctgcactggg tcgtgccgct
     1201 gggcgaccct ggagcgagcg actggggtta tcagttcacc atcccactgt ggtacatccg
     1261 ggcctacttc tggtttctgc tgctctctcc cgccgcgctg ttcatgttcc gacgctggcc
     1321 taagcgaacg atgtcggtgc cgctgatcgt ggcggttctc agcggccttg gggtgttcga
     1381 tctcagtctg cagggttcga cgtccagcgc gatcgtcgcc acgatggcga cgttcggtgc
     1441 gtgctggatg atcggcttcg cgcatcacga cggcacgttg cgcaacctgt cgctgcgctg
     1501 ggtcatcccc gcgtcgctgg catcgatcgc catcggcacg tggtgggccg ctacccgagc
     1561 cagctgggcg ctggacgacg acaccttggg ccaagcgctc tactgcttgg gcttcgtgct
     1621 cctgctgctt cgcttctatc cctccttcca gtggctgcag cgccgcccac tcctggacaa
     1681 actgatcgcc gtcgtcaact cgcgtgcgat gaccatctat ctgtggggca acgtcgcgat
     1741 cttcgccgct ggcgtgctca tcagcagcgg atggatgccg aggatcgtca cctccaacga
     1801 cgtcaccgcc ggcctgagcc gcctcgtcct ggctctcgtg gtgatcgccc tggcggtcct
     1861 ggcggtcggt tgggtcgagg acgtcgccgc tcaccggacc cctcgcctca acccatggcc
     1921 gagaacgctc cccgagcacg gagccatgac ctcgagcgga ctcctacggc ctgcggacgg
     1981 atcccctcag gtgcacctca ggctgccccg gcggcgtctg gccggcgttc tgctcatggc
     2041 cggtctcgcc gcgaccctca cactgctctg gaccgccggc gaccgcagcg gggtcgcacc
     2101 cagaaccacc gagcagggac aaacc
//
LOCUS       DATADN010001052         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_439364, whole genome shotgun sequence.
ACCESSION   DATADN010001052 DATADN010000000
VERSION     DATADN010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439364"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VHH53_13510"
     CDS             <1..399
                     /locus_tag="VHH53_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2291179.1"
                     /translation="TLRADGSPRISGIEASFTQGQLWFGSMPDARKGQDLRRDPRFAL
                     HSASVDPPEGQESSWSGDAKISGRAVLVTDPGERDALLQAQGAPPGQGPDVFRADIDE
                     VVITKVGEPADHLMIELWRPDRPLHHIQRR"
     gene            complement(409..>1719)
                     /locus_tag="VHH53_13515"
     CDS             complement(409..>1719)
                     /locus_tag="VHH53_13515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX2291180.1"
                     /translation="LDVLAAVRGRDAYANLVLPGLLRQRGINGRDAALATELCYGTCR
                     AQGLLDAVLESCTDRPLSEVDGELLDALRLGSYQLLRTRVPSHAAVGTTVDLVRADRG
                     SASAGFVNAVLRRVSQHDEQGWVQAVAPAAGDDPVGYLAMAHPHPRWIAQAFSDALGG
                     DSDELSQALAADDGRPLVHLAARPGRITATELATVTGGQAAPYSPYGVHLADGGDPGE
                     LAAVRDHLAHVQDEGSQLVALALAGVQMEGADVRWLDLCAGPGGKAALLSAVGAARGA
                     SLDAVEKAEHRARLVGQACVGLPVTVHVADGRNSQLPERSYDRVLVDVPCTGLGALRR
                     RPEARWRRRADDLAGLVRLQRELLLAALRLVRPGGVVAYVTCSPHLDETVGVVADVVR
                     RTGAQQLDARPQFPGVPGLGQGPHVQLWPHRHGTDAMFCALLRR"
BASE COUNT          270 a          642 c          584 g          223 t
ORIGIN      
        1 acgctgcggg ccgacggctc accgcgaatc agcgggatcg aggcgtcgtt cacgcagggt
       61 cagctgtggt tcgggtcgat gccggacgcc cgcaaggggc aggacctgcg gcgcgatccg
      121 cggttcgcgc tacacagcgc ctcggtggac cccccggagg gtcaggagtc cagctggtcg
      181 ggggacgcga agatcagcgg ccgggcggtg ctggtcaccg accccggtga gcgagacgct
      241 ctgctgcaag cgcagggtgc tccacccggc cagggtcctg acgtgttccg agctgatatc
      301 gacgaggtcg tcatcaccaa ggtcggcgag ccggcggatc atctgatgat tgagctgtgg
      361 cgcccggacc ggccgctgca ccacatccag cgacgctgaa gggccacttc aacggcgcaa
      421 cagggcacag aacatcgcat cggtgccgtg ccgatgcggc cacagctgca cgtgcggtcc
      481 ctgcccaagg ccgggtacgc cggggaactg cggtcgagca tcgagttgct gcgcaccggt
      541 acgccgcacc acgtcggcga ccacacccac ggtctcgtca aggtgcggcg agcacgtcac
      601 gtaggccacc acaccccccg gacgcaccag tcgcagcgcc gccagcagca gctcacgctg
      661 taaccggacc agcccggcga ggtcatcagc gcgccgtcgc caacgcgcct ccgggcgccg
      721 gcgcagcgcc ccgagcccgg tgcacggtac gtccaccagc acccggtcgt aggaccgttc
      781 cggcagctgg ctgttgcggc cgtcggcgac atgcaccgtc accggcaggc ccacgcaggc
      841 ctgaccgacc aaccgggccc ggtgctcagc cttctccaca gcgtcgaggc tggccccgcg
      901 agcggcaccc acggcgctca gcaacgctgc cttgcctccc ggtccggcgc acaggtccaa
      961 ccagcgcacg tcagcgccct ccatttggac cccggccagt gcgagggcga caagctggct
     1021 gccctcgtcc tgcacatggg ccaagtgatc gcgcaccgcc gccagctcac ccgggtcacc
     1081 gccatcggct aggtgcaccc cgtacggcga gtacggggcg gcctggccgc cggtcaccgt
     1141 ggccagctcg gtagcggtga tccggcccgg gcgcgcggcg aggtgcacca gtgggcggcc
     1201 atcatcggca gccagcgctt ggctcagctc gtcggagtcc ccacccagcg cgtcggagaa
     1261 ggcctgtgcg atccaccgcg ggtgcgggtg ggccatcgca aggtagccca ccgggtcgtc
     1321 tccggctgcc ggcgccactg cctgcaccca accttgttcg tcgtgctggc tgacgcggcg
     1381 caacaccgcg ttgacgaagc cggcgctagc actgccccga tccgcgcgca ccaggtcaac
     1441 ggtggtgccg acggcggcat gcgacggtac ccgggtccgc agcagttggt agctgcccaa
     1501 ccgcagcgca tccagcagtt cgccgtccac ctccgacagc ggacgatcgg tgcaggactc
     1561 caacaccgca tccagcaggc cctgcgcgcg gcaggttcca tagcacagct cggtggccag
     1621 cgcggcgtcc cggccgttga tcccgcgctg ccgtagcagg cctggcagca ccaggttggc
     1681 atacgcgtcg cggccgcgca ccgcggccag gacgtccag
//
LOCUS       DATADN010001053         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_871997, whole genome shotgun sequence.
ACCESSION   DATADN010001053 DATADN010000000
VERSION     DATADN010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871997"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VHH53_13520"
     CDS             complement(<1..289)
                     /locus_tag="VHH53_13520"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: protein motif:HMM:NF014612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX2291181.1"
                     /translation="MTRVQHPIEIESYRILRSKVNTGALPPRTRAVIERVIHTSADLD
                     YATDLICDEEALRAGQQALAAGTPLVVDVRMLAAGITSRRCVVAIDLADDPP"
     gene            356..598
                     /locus_tag="VHH53_13525"
     CDS             356..598
                     /locus_tag="VHH53_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291182.1"
                     /translation="MSVEDHDDELATQYVLARRLRPDLDGPELAKLIMSRLNEEQLMR
                     YAEDALAWAPHPADRQELALRYVLNFVLAMETDTDE"
     gene            752..1348
                     /locus_tag="VHH53_13530"
     CDS             752..1348
                     /locus_tag="VHH53_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX2291183.1"
                     /translation="MATAAVAIGPMACGYQGLTLPAQSAITLRGQGALTEPKPDSNTV
                     TYNPELAPPGARMAVTMIPSGTSTNVELTVNGLQPNRGYAVHAHENLCSENPQFAGGH
                     YQNRVDPAASPTQPSTNPEYANPNNEIWLDLRTDAAGSGTSRTTVPFVFTDRGPGSVV
                     IHEAEQTSTAPGQAGEAGARVACLSLAADRPQDNTLLS"
     gene            1396..>1810
                     /locus_tag="VHH53_13535"
     CDS             1396..>1810
                     /locus_tag="VHH53_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2291184.1"
                     /translation="MRPATGLISFVGAGPGAADLITLRGAHRLASADVVVWASSLVPA
                     AVLEHCRDGVELHDSAGMTLEDVLAVYEAHPDAAIVRLHSGDVSLYSALTEQIAWCAE
                     HERTFEIVPGVGSLAAAAASAGVELTVPGVAQSAVL"
BASE COUNT          339 a          591 c          582 g          298 t
ORIGIN      
        1 cgggcgggtc atcggccagg tcgatcgcga ccacacaccg ccgcgacgtg atgccagcag
       61 caagcatccg gacatcgacg accagtggag tccctgcagc taatgcctgt tgtccggcac
      121 gcagcgcctc ctcgtcacaa atcaggtcag tggcatagtc gaggtccgcg ctggtgtgga
      181 tcacccgctc aatcactgcg cgggtgcggg gcgggagtgc cccggtgttc actttcgatc
      241 gcaggatccg gtatgactcg atctcgatcg ggtgttggac ccgcgtcacg tcatttcctc
      301 tctgcctcta gcagattgtg gccacactag gtgcagggcc gaacggagac cgcagatgag
      361 cgtcgaagat cacgacgatg aactggccac ccagtacgtg ctcgcccggc gcctgcgtcc
      421 cgatctcgac ggacctgagc tcgccaaact gatcatgtca agactcaacg aggaacagtt
      481 gatgcgctac gccgaagatg ccctcgcttg ggcaccgcac ccggccgacc gccaggaact
      541 tgcactgcgc tacgtgctga acttcgtcct tgccatggaa accgacacag acgagtagtt
      601 aggggtcgcg cagcgcatac aggtcgggca atatacatcc atgaggggac attcttcgat
      661 tgatcaaagt cgcacagggt attcaagggc gggaacctgt cagcagccag tgactgctcg
      721 tcgaccgatg tatcgacggc tcacactggc tatggccacc gcagcagtcg ccatcgggcc
      781 gatggcgtgc gggtaccagg gcctgaccct gcccgcccaa agcgcgatca ctctgcgggg
      841 ccagggcgcg cttaccgaac ccaagcccga ctccaacacc gtgacgtaca acccggaatt
      901 ggcgccgccc ggagcgcgga tggcagtcac catgatcccg tcgggaacgt cgaccaacgt
      961 ggagctcacc gtcaacgggc tgcagcccaa ccggggatac gcggtgcacg cgcacgagaa
     1021 cctttgcagt gaaaatcccc agttcgcggg tgggcattac caaaaccgcg tcgatcccgc
     1081 cgcctcgccg acacaaccat caaccaaccc tgaatacgcc aacccgaaca acgagatctg
     1141 gttggacctg cgcactgatg cagcaggctc aggcacgagc cggaccacgg tgcccttcgt
     1201 cttcaccgac cggggacccg gatcggtcgt gattcacgag gcggaacaga cctctaccgc
     1261 cccgggccag gcaggcgagg caggtgcccg ggtcgcgtgc ctgagtctgg ccgcggatcg
     1321 gccgcaggac aatactctgc tgagctagga ggactggcca cctcgtatgg ggtccccggg
     1381 agatgggatg gcctgatgcg accggctacg gggctgatct cgttcgtggg cgccggacct
     1441 ggcgcggcgg acctgatcac cctgcgtggg gcgcatcggc tcgcgagcgc cgacgtggtg
     1501 gtctgggcat cgtctctggt gcctgccgcg gtactcgagc actgccgcga cggcgtcgag
     1561 ctgcacgact cggcagggat gacgttagag gacgttctcg cggtgtacga ggcgcacccg
     1621 gatgcggcga tcgtgcggct gcattctggg gacgtctcgc tgtactccgc gctgaccgag
     1681 caaatcgcct ggtgcgccga gcacgagcgc actttcgaga tcgtgcccgg ggtggggtca
     1741 ctcgccgccg ccgcggctag tgccggggtc gaactcaccg tccccggagt tgcgcagagc
     1801 gcggtactca
//
LOCUS       DATADN010001054         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_270865, whole genome shotgun sequence.
ACCESSION   DATADN010001054 DATADN010000000
VERSION     DATADN010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270865"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VHH53_13540"
     CDS             complement(<1..456)
                     /locus_tag="VHH53_13540"
                     /inference="COORDINATES: protein motif:HMM:NF016349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HEX2291185.1"
                     /translation="MADHLLRKYAPIPATGWDQIDDEAKTRLTPRLAARRLVDWSGPH
                     GWTHSATNLGRTEPVNTAPPGTKSETVMARRRRVLALSEFRVSFTVDCSELQDTERGA
                     TDVDFADLDRATYDAALIENRAIFHGWPEAGITGITEASAHPPLSLGTNV"
     gene            complement(459..1016)
                     /locus_tag="VHH53_13545"
     CDS             complement(459..1016)
                     /locus_tag="VHH53_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX2291186.1"
                     /translation="MADITQLILDDHDTFRREFAALDDAQGPEQLRAIWEPLAALLDV
                     HAVAEETVFYPQLLRRGDEAEDETLDAIGDHNDIRDGVHEAARYPIGSPQWWDGVRKA
                     RMANSDHMAEEEDEALADFRRNAPLDVREELGHQFLKFKAEHADARDLDTTDKDPEGY
                     VSAVQAEISPGSGSGSLGIGSLKGR"
     gene            complement(1062..1475)
                     /locus_tag="VHH53_13550"
     CDS             complement(1062..1475)
                     /locus_tag="VHH53_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX2291187.1"
                     /translation="MTVAAEMIRSYPADLGGVDRDALIRCIEECVSCAQACTACADAC
                     LSESDDQLPMLRKCIRSDEDCADMCNATARMLSRHTGYDANVTRTVLQACIVACRSCG
                     DECASHASRHEHCRVCAEACRRCEQACQELIEALG"
     gene            complement(1698..>1899)
                     /locus_tag="VHH53_13555"
     CDS             complement(1698..>1899)
                     /locus_tag="VHH53_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEX2291188.1"
                     /translation="LSDLQQPVGWLGYLASADFWNRTLQNWQSEFLAIGSMVVLSVYL
                     RQRGSPESKPVGAPHKVTVDSG"
BASE COUNT          302 a          630 c          600 g          367 t
ORIGIN      
        1 gacgttggtg cccagcgaca gcgggggatg agcgctggct tcggtgatgc cggtgatccc
       61 cgcttcgggc cagccatgga aaatggcgcg gttctcgatc aaggcggcgt cgtaggtggc
      121 tcggtcgaga tcggcgaagt ccacatcggt agccccgcgt tcggtgtcct gcagctctga
      181 gcaatccact gtaaagctca cccggaactc gctcagcgcc agcacccgcc gccggcgggc
      241 catgaccgtc tcggacttcg tccccggcgg tgcggtattc accggctcgg tccggcccag
      301 gttggtcgct gaatgtgtcc atccatgcgg cccggaccag tcgaccagcc ggcgtgcggc
      361 caacctcggt gtcaggcgcg tcttggcttc atcatcgatc tgatcccatc cggtggctgg
      421 gatcggtgcg tacttccgta acaggtggtc cgccatcgtc agcgtccttt caaactaccg
      481 atacctaatg agccgctccc actaccggga gagatctccg cctgtaccgc gctgacgtat
      541 ccctccgggt ccttgtcagt ggtatccaga tcccgggcat cggcgtgctc ggccttgaac
      601 ttcaggaact ggtgacccag ctcctcgcga acatcgaggg gggcattccg ccggaaatcg
      661 gcgagggctt cgtcctcctc ctcggccatg tgatcgctgt tggccatccg ggcctttcga
      721 acgccgtccc accactgcgg actaccgatc gggtatcggg cggcctcatg cacgccatcg
      781 cggatgtcgt tgtggtcgcc gatcgcatcg agggtctcgt cctcggcctc gtcaccgcgg
      841 cgcagcagct gcgggtagaa gacggtttcc tcggccactg catgcacgtc gagcagcgct
      901 gcgaggggtt cccagatcgc gcgtagctgc tcgggtcctt gagcatcgtc tagggcggcg
      961 aactctcgcc gaaaagtgtc gtgatcgtcg aggatcagct gggtgatgtc ggccattgca
     1021 cggtgctcct tgttgtgggc tccttgcctt gcgtgtacgc ctcagccgag ggcttcgatc
     1081 agctcctggc atgcctgctc gcaacggcgg cacgcttcgg cgcagacccg gcagtgctca
     1141 tgccgggagg cgtggctggc gcactcgtcg ccgcacgagc ggcaggcgac gatgcaggcc
     1201 tgcaagacgg tacgggtcac attggcgtca tagccggtgt gccgggacag catgcgcgcc
     1261 gtggcgttgc acatgtcagc gcagtcttcg tctgaacgga tgcacttacg caacatcggt
     1321 agctggtcgt cagactcgga caggcacgcg tcagcgcatg cggtacacgc ctgcgcgcag
     1381 gacacacatt cttctatgca gcgaatcaga gcgtcgcggt cgaccccgcc caggtctgcc
     1441 gggtaactac ggatcatttc tgccgcaacg gtcatcctta cctcctggtc tcaacctccc
     1501 ttcggttacc cgccatggcg cgcaccacac ctaccccgca gcagttgttc gaacttcgag
     1561 ttcgttgcta cccgggcgta cacgatctcg tcggcggcgg tagcgggctc accggagtgg
     1621 cggccaccgg acgatctggc cccaactccg agcggacgtc agggggctgc tcttccagcc
     1681 gctcggccag tgaccgctca gccggagtcg acggtcacct tatggggggc tccgaccggc
     1741 ttggattccg gcgaaccacg ctggcggagg tagacactga gcaccaccat cgagccgatt
     1801 gccaggaact ccgactgcca gttctgcagc gtgcggttcc agaagtctgc gctggccagg
     1861 taccccagcc agcccacagg ttgctgcaga tcgctaagc
//
LOCUS       DATADN010001055         3378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_270881, whole genome shotgun sequence.
ACCESSION   DATADN010001055 DATADN010000000
VERSION     DATADN010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270881"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..916)
                     /gene="fabI"
                     /locus_tag="VHH53_13560"
     CDS             complement(149..916)
                     /gene="fabI"
                     /locus_tag="VHH53_13560"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX2291189.1"
                     /translation="MAGLLDGKRLLITGVITDASIAFHVARVAQEQGAQVVLTGYGRL
                     RLVERIAKRLPQPAPVLELDVQDQTQLDSLAQRLGEHVDGLDGVLHSIGFAPASCLGG
                     EFLNAPWEDVATAMHISAYSFKSLAMACRPLLHRGASIVGMDFDNRQAWPAYDWMGVA
                     KSALESVCRYLARDLGPDGIRVNLVAAGPVRTMAAKSIPGFAAFENAWSGRAPLGWNI
                     DDPTPVARTACALLSDWLPATTGSMVWADGGFHAVGV"
     gene            complement(978..1694)
                     /gene="fabG"
                     /locus_tag="VHH53_13565"
                     /pseudo
     CDS             complement(978..1694)
                     /gene="fabG"
                     /locus_tag="VHH53_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
     gene            complement(1760..2743)
                     /locus_tag="VHH53_13570"
     CDS             complement(1760..2743)
                     /locus_tag="VHH53_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2291190.1"
                     /translation="MSLGGFTSPFWFLLLVVIAALAAGYVWMQRRRRRQVLRFANLAM
                     LERVAPRRQGWQRHLPLALLLTALVLFTVALAGPTAEQKVPRNRATVMLVIDVSLSMR
                     ATDIEPSRLAAAQREAKRFAHELTPGVNLGLVSFAGSPTVLVSPTTERVSVVRAIDNL
                     KLAEATGTGEAIFAALSSIEAFGAVVSDAQGPPPARIVLLTDGKQTVPTPDGDDPRGG
                     FTAGRAAAAAKIPISAISFGTAYGQVDINGERQPVPADDESIRRIAELSGGQFYDAGS
                     AGQLSDVVNTLGEQIGYETKRAEASKPWLIAGTIVAMLAAAGALAIGQRLP"
     gene            complement(2740..3378)
                     /locus_tag="VHH53_13575"
                     /pseudo
     CDS             complement(2740..3378)
                     /locus_tag="VHH53_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638317.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
BASE COUNT          548 a         1183 c         1152 g          495 t
ORIGIN      
        1 cagcaccagt agtgcatcag tcaaacggcg ctccttcagt tgcgctcgtg agtgcgtaac
       61 agtgttttag cactgttacg cactcacgag gttggtagct gttggagacc cttccgagtg
      121 gggttggcgg tacgtgaagg cgtgccaact agacgccgac cgcgtggaag ccgccgtcgg
      181 cccacaccat ggaaccggtc gtggcaggta gccaatcgga cagcagcgcg caggcggtgc
      241 gagccaccgg agtggggtcg tcgatgttcc agcccagtgg agcgcgacct gaccaggcgt
      301 tctcgaacgc ggcgaagccc gggatcgact tggccgccat ggtgcgcacc ggcccggctg
      361 ccacgagatt gacccggatg ccgtcggggc cgaggtcgcg agccaggtag cggcagaccg
      421 actccagcgc cgacttcgcc acacccatcc agtcataggc tggccaggcc tgccggttgt
      481 cgaagtccat cccgacgatc gacgcgccgc ggtgcagcag cggtctgcac gccatcgcca
      541 gcgacttgaa cgagtacgcc gagatgtgca tcgcggtggc cacgtcctcc cagggtgcgt
      601 tgaggaactc cccgcccagg cacgacgcgg gcgcaaagcc gatcgagtgc agcaccccat
      661 ccagcccgtc gacgtgctcg ccgagtcgct gcgccagcga gtccagctgg gtctgatcct
      721 gcacgtcgag ctcgagcacc ggcgccggct gcggcaggcg cttggcgatg cgctcgacca
      781 gacgcaaccg gccgtacccg gtgagcacca cctgggcccc ctgctcctgg gccacccgcg
      841 ccacgtgaaa tgcgatcgag gcgtcggtga tcaccccagt gatcagcaaa cgcttaccgt
      901 cgagcagacc cgccacctgt tctcctcccc tcgattgccg tgtttcagtg ccgccgtaac
      961 tcagtgacgc ttgaaactca gtgacccatt cccaggccgc cgtccacagg caataccgcg
     1021 ccggtgatat atcccgccgc gtccgaggcg aggaaggtca ccacggcagc gacctcctcg
     1081 gggctggcct ggcggcgcag cgggatcgag gccaggatct cctcccggcg cgcctggctc
     1141 agctccgcgg tcatgtcggt gtcgacgtaa ccgggcgcga ccacattggc ggtgatcgac
     1201 cgggatccga gctcacgggc gatggaccgg gcgatgccga tcagcccggc tttgctggcc
     1261 gcgtagttca cctgcccggg gccgccggac agcgcgacca ctgaggagat gaacaccatc
     1321 cggccacggc gggcgcgcag catgctcttg gcggcgcgct tggctacccg gtaggcaccg
     1381 gtgaggttgg tgtccaaaac ccgcgcgaac tgctcctcag ccatccggag cagcagcgtg
     1441 tcatcggtga ttccggcatt ggagatgagc acctcgatcg gaccgtggat gccctccacc
     1501 tcggcgaacg cggcatctac ggcagcgccg tcggtgacgt cgcaccgcac cggcaacagc
     1561 tcgggcgcga gtccctccgg aacactggtt ccgcggtggg tgaccgcgac ccggtcgccc
     1621 tgctagggga aggcgcgggc gatggccagc ccgataccac ggttacctcc cgtgacgagg
     1681 acggaacggg tcaaccggcg ctcccttcac tcacgggtgt tttgccggcg acggtaccgg
     1741 agcgtccgtt cctggcagct cagggcagcc gttgtccgat ggccagcgca ccggcagcgg
     1801 cgagcatggc gacgatggtt cccgcgatca gccacggctt gctcgcttcg gcccgcttgg
     1861 tctcgtagcc gatctgctcg cccagggtgt tgacgacatc gctgagctgg cccgcggagc
     1921 cggcgtcgta gaactgcccg ccggacagtt cggcgatgcg ccggatcgac tcgtcgtcgg
     1981 cgggcaccgg ctggcgctcg ccgttgatat ccacctgacc gtaggcggtg ccaaaggaga
     2041 tcgccgagat ggggatcttg gcggcggccg cggcgcggcc cgcggtgaac cccccgcggg
     2101 gatcgtcgcc gtcaggggtc ggcactgtct gcttgccgtc ggtgagcagc acgatccgcg
     2161 ccggtggcgg tccctgcgca tccgagacca ccgccccgaa cgcctcgatc gacgacagcg
     2221 cggcgaagat cgcctctccg gtgccggtgg cctcggccaa cttgaggttg tcgatggccc
     2281 ggaccaccga aacccgctcg gtggtgggcg acaccagcac ggtgggcgag ccggcgaagg
     2341 acaccaggcc caggttcacc cctggggtga gctcgtgggc gaacctcttc gcctcccgct
     2401 gggcggccgc gagcctgctg ggttcgatat cggtggcccg catggacagg gacacgtcga
     2461 tcaccagcat gacggtggcc cggttgcgcg gcaccttctg ctccgcggta ggaccggcca
     2521 gcgcgacggt gaacagcacc agggcggtca gcagcagcgc tagcggcagg tgtcgctgcc
     2581 acccctggcg cctgggggcg acgcgttcaa gcatggcgag attggcgaag cgcagtacct
     2641 ggcggcggcg ccgacgttgc atccagacgt agccagcggc cagcgcggcg atcaccacca
     2701 gtaggaggaa ccagaaagga ctggtgaatc cgcccaggct catgcgcgcc ctcccgacca
     2761 gccgcgcttg cgggcgaccg cgaagcgcac catgtcggac acccagtccg aatcggtgcg
     2821 cagcgtcagc tgcgctgcac cgcatcgccg cagcgtcgcg gccacctcct cacgatgcgc
     2881 agcggccgca gcggcgaact cccggcgcag caacggcgtg gtcacgacct ccttttgccg
     2941 cccggtctct ggatctgcga gcaccacggt gccgatgtcg ggcagttcga gatcacgcgg
     3001 gtccagcacc tcgatcgcga tcaggtcgtg gcgggccgac agcccacgca acggccgtta
     3061 ccaatcgaaa acactcccgg accgcgggcc gaggaagtcc gacaccaccg cgaccaaccc
     3121 gcggcggcgc ggggggcggc gtagctgttc cagtgtcccg gccagatcac ctcggacgcc
     3181 ttcagcagcg cgcggtagct cggcgatccg gcgaatgatc ccgcgggcgt ggggcaagcc
     3241 accacgagcg ggaatgcgca cgacatccgc gccggtgctg gcgaccacgc cgactcggtt
     3301 gccgccgccc cgggtcaggt gggccacagc ggcgcaggcg gccaccgcga gctcgcgctt
     3361 gtcgcattcg gcggtacc
//
LOCUS       DATADN010001056         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_270948, whole genome shotgun sequence.
ACCESSION   DATADN010001056 DATADN010000000
VERSION     DATADN010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270948"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1013)
                     /locus_tag="VHH53_13580"
     CDS             complement(141..1013)
                     /locus_tag="VHH53_13580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2291191.1"
                     /translation="MSPRNSYGQELAVVTVTYSPGDTLETFLDSIAKATSRPLQVLLA
                     DNGSTDGAPERALCRDGVQLLRIGENVGYGSAANRGVAEFGPEVGWVVVANPDIEWHP
                     GSLDELLAAGDRWPRAGALGPLIRQPDETVYPSARLLPALGRGIGHALFGQVWPGNPW
                     TTIYRQSDTALGERTAEWLSGSCLLLRRAAFDSVGGFDSRYFMYLEDVDLGDRLVQAG
                     WLNVYVPTAQVTHLGGHATSRVSELMLAEHHHSMYRYLADRHVGALRAPLRLALRAGL
                     AGRSQLVRRAARRG"
     gene            complement(1010..>1612)
                     /locus_tag="VHH53_13585"
     CDS             complement(1010..>1612)
                     /locus_tag="VHH53_13585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2291192.1"
                     /translation="ATQYVAFLGALEPRKNVPALIGGFAQAHRALGSRPDPPVLVLAG
                     QPGWDGQVERALQAVPHRVRVIRAGYLPLSELSGFLGGAALVAYPSLGEGFGLPVLEA
                     MAAGAAVLTTRRLAIPEVGADAVAYCGVAAEDIAAGILDLIDDPAGRAELSAAAQRRA
                     KEFSWAASAVLHRQAYGRAAVAHRRPASFAGRSRVQNRAL"
BASE COUNT          267 a          590 c          523 g          232 t
ORIGIN      
        1 gttgcggtca gaggtgatct tggttcaggt gcgcaggcgc ctgcgctggc cttcgtactg
       61 gacctctcga caaacctgat cgaccccacc gtggcgtcgt ccagctccag cgtcagccgc
      121 tccgcgcact cagcgaggcc ctacccacgc cgggcggcgc gacgcaccaa ctgagaccgg
      181 ccagccaacc cggctcgcag tgctagccgc agcggagcac gcagtgcacc gacgtgccgg
      241 tcggcgaggt agcggtacat gctgtgatgg tgctcggcga gcatcagctc ggagacccgg
      301 ctggtggcgt gtcccccgag gtgggttacc tgcgcggtcg gcacgtagac attgagccac
      361 cccgcttgca ccaagcgatc cccgaggtca acgtcctcca agtacatgaa atagcgggag
      421 tcgaagccgc cgacggagtc gaaggcggca cggcgcaaca gcaggcagga acctgacaac
      481 cactccgcgg tccgctcgcc caaggcggtg tcggactgcc ggtagatcgt ggtccaagga
      541 tttcccggcc atacctggcc aaacagcgca tgcccgatgc cgcgacccaa cgcgggcagc
      601 agccgtgccg acgggtacac cgtctcgtcg ggttggcgga tcagcggccc cagtgcacct
      661 gccctgggcc atcgatcccc ggccgcgagc agttcgtcga ggctacccgg gtgccactcg
      721 atgtccggat tggccaccac cacccagccc acttccggtc cgaactccgc aaccccacgg
      781 ttggcggcgc tgccgtatcc gacgttctcc ccgatgcgca gcaactggac accgtcgcgg
      841 cacagcgcgc gctccggggc tccgtcggta gatccgttgt cggccagcaa cacctgcagc
      901 ggcctgctgg ttgctttggc gatcgaatcc aggaacgtct ccagcgtgtc accgggcgag
      961 tacgtcaccg tgacgacggc aagttcctgg ccgtaggaat tgcgggggct cacagcgccc
     1021 gattctgcac ccgggaccgc ccggcgaagc tagcgggacg ccggtgcgcc acagcggccc
     1081 gaccgtaggc ctgccggtgc agtaccgcgc tcgccgccca agagaactcc ttcgcgcgcc
     1141 gctgcgccgc ggccgacagt tcggcccggc cggctgggtc atcgatcagg tcgaggattc
     1201 cggcggcgat gtcctcggct gcgacaccgc agtaggccac tgcgtccgca cccacctcgg
     1261 ggatcgctag ccggcgcgtg gtgagcaccg ccgcgccggc tgccatcgcc tccagcaccg
     1321 gtagcccgaa cccctccccc aacgacgggt aggccaccag tgctgcgcca ccgaggaacc
     1381 cggacagctc actcaacggc agataaccgg cccggatcac ccgcacccgg tgcggcacgg
     1441 cctgcagtgc gcgctccacc tggccgtccc agcccggttg cccggccagc accaggacag
     1501 gggggtcggg cctgctgcca agggcacggt gtgcttgagc gaacccaccg atcaacgcgg
     1561 gcacgttctt gcgtggttcg agtgctccga ggaaagccac gtactgcgtt gc
//
LOCUS       DATADN010001057         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_439703, whole genome shotgun sequence.
ACCESSION   DATADN010001057 DATADN010000000
VERSION     DATADN010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439703"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..997
                     /locus_tag="VHH53_13590"
     CDS             272..997
                     /locus_tag="VHH53_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX2291193.1"
                     /translation="MTTETVAMHMSDGIVNAPTSAVLGLVAAAGLAYSLVRARAGQSA
                     DLDNRTAPMAGLVAAFIFAVQMINFPVLPGVSGHLLGGALAAILVGPWLGALCVGIVL
                     VVQALLFADGGLTALGANITNMALIGTWVGFAVALALRGLAIRSRGWLVTVAFVSALV
                     NTVIASLGFVVAYAVGGAGGSAVATVAVAILGVHVLIGIGEGIITALTVGAVASARPD
                     LVYLLWQSPARTATTATAERSAS"
     gene            994..1377
                     /locus_tag="VHH53_13595"
     CDS             994..1377
                     /locus_tag="VHH53_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HEX2291194.1"
                     /translation="MTAPHRRRSPTGFFVAFGVIALLIAGLLSYLASRNPDGLDSATL
                     NGCQVTEVDGTEQLSGQCIAQNARDHDLAGSPLADYTLGGDDGLVGIAGVIGVLVTLA
                     LAGGLFWLARPRQERGAGKRSAGKT"
BASE COUNT          220 a          520 c          490 g          277 t
ORIGIN      
        1 gtgacgttgt atgcgtccat gaggtccaac gcgcggctgc tctgctcgtc aagctccggg
       61 tcgtcgcgat gtagaggtca ccagcgagca tccgtgaccg catcgagcgg gaatccgggg
      121 agctcggcga cggcatatcg accaccgtac gcatcggtcc caaagcatcc gctacaggcc
      181 atatacaggc tttcgcatag gtatagcagt aacacagctt atgcctttac tctgacgggt
      241 gacccccaca agcgtccccg aggacggcac gatgactaca gagacggtcg ccatgcatat
      301 gagcgacggc atcgtcaacg cccccacctc ggcagtgctc ggcctcgtcg ccgcagcagg
      361 gctcgcctac tcgctggtcc gcgcccgggc gggccagagc gcggacctgg acaatcgcac
      421 cgcaccgatg gcgggcttgg tcgcggcgtt catcttcgcc gtgcaaatga tcaactttcc
      481 ggtgctgccc ggagtcagcg ggcacctctt gggcggggcg ctggccgcca tcctggtggg
      541 cccctggctc ggcgctctgt gtgtcggcat cgtgctggtg gtgcaggcgc tgctgttcgc
      601 cgacggtggt ctcaccgcgc tgggcgccaa catcacgaac atggcgctga tcggcacctg
      661 ggtcggcttc gcggtcgcct tagccctgcg cgggctggct atccgcagcc gcgggtggct
      721 ggtcaccgta gccttcgtgt ccgccctggt gaacacggtg atcgcatccc tgggattcgt
      781 cgtggcatac gcggtcggcg gggccggcgg ctccgctgtg gctaccgtcg cggtggccat
      841 cctcggagta catgtcctga tcggcattgg cgagggcatc atcaccgcgc tcaccgtcgg
      901 cgctgtcgcc tccgctcgcc cggacctcgt gtacctgctg tggcagtcgc cggcaaggac
      961 cgcaacgacc gcgactgctg agcggagtgc atcatgaccg ccccgcaccg gcggcgcagc
     1021 cctaccggat tcttcgtcgc cttcggcgtc atcgcacttc tgattgccgg gctgctctcc
     1081 tacctggcct caagaaaccc agacggcctc gactcggcga ccttgaacgg ctgccaggtc
     1141 actgaggtgg acggcaccga gcagctctcc ggacagtgca ttgcccagaa cgcccgcgat
     1201 catgacctgg ccgggagccc ccttgctgat tacaccctgg gcggtgacga cggcctcgtg
     1261 ggaatcgccg gggtcatcgg ggtgctggtg acccttgccc tcgccggcgg cctgttctgg
     1321 ctggcgcgcc cgcgccagga acgcggcgcc ggcaagcgct cggctggcaa gacctgatcc
     1381 gccgtgggag ccggccacgc acatccactt ttcctaccgg gtaattcacc ggtgcaccgg
     1441 ctgccacctc acgtcaaggt gcttgccgca ttcctgttcg tgtgctgcgt ggtggctacg
     1501 ccacggg
//
LOCUS       DATADN010001058         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1193000, whole genome shotgun sequence.
ACCESSION   DATADN010001058 DATADN010000000
VERSION     DATADN010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193000"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..951)
                     /locus_tag="VHH53_13600"
     CDS             complement(82..951)
                     /locus_tag="VHH53_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2291195.1"
                     /translation="MKPSRSYRVFQGVNGVVLTLVVLVTLYPIVNILARSFSGERQIR
                     AGEVTLLPKGFNLTTYEIVFKDSMFWRNYGNTVLYTVVATAVAMVLTTCYAYVLSKKH
                     LKGRGVLVGIAVFTMFFTGGLIPNYILVTSLGLKNSVWAIALPNAISVFNLLVMKAFF
                     ENLPSELEEAAQIDGLGTYGILARIVLPLSKAVVATMVLFYSVSFWNSWFSAFLYMDR
                     TDLMPVTVYLRNLIQGATGGGNAGAGTEQLSQVGANIQSVTIVLTALPILCVYPFVQR
                     YFVSGVMLGAVKG"
     gene            complement(948..1100)
                     /locus_tag="VHH53_13605"
     CDS             complement(948..1100)
                     /locus_tag="VHH53_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291196.1"
                     /translation="MISTYVYRSGVESNSFSYAAAIGLFEAVIGLILITSANQLSRRT
                     VGTSLW"
     gene            complement(1118..1429)
                     /locus_tag="VHH53_13610"
     CDS             complement(1118..1429)
                     /locus_tag="VHH53_13610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2291197.1"
                     /translation="MADRRVGHDPIRFIQEPEWFRTIYVGSEIWQTAGWGTILYLAAL
                     TTIDEDLYEAARIDGANHWQQIWHVTLPGIRPTMVTLLILNVGAFMAVGSRRSCCCTT
                     R"
BASE COUNT          332 a          523 c          580 g          267 t
ORIGIN      
        1 gcgagtatct gacgccggga gagctgtcct gcgttctgca cggagactcc tttgttccgt
       61 aggcgagttg aggaggtggc gtcagccctt gaccgcgccg agcatcacgc ccgagacgaa
      121 gtagcgctgg acgaacgggt acacgcagag gatcggcagg gcggtgagga cgatggtcac
      181 cgactggatg ttcgcgccga cctggctgag ctgctccgtg cccgcgccgg cgttgccgcc
      241 accggtggcg ccctggatga ggttgcgcag atagacggtg accggcatca agtccgtccg
      301 gtccatgtag aggaaggcgc tgaaccagga gttccagaag gacaccgagt agaacagcac
      361 catcgtcgcg acgaccgcct tggacagcgg cagcacgatc cgggcgagga tgccgtaggt
      421 gccgaggccg tcgatctggg cggcctcctc cagttcgctc ggcaggttct cgaagaaggc
      481 cttcatgacc aggaggttga agacgctgat cgcgttgggc agggcgatcg cccagacgct
      541 gttcttcagg ccgaggctgg tgaccaggat gtagttgggg atcaggccac cggtgaagaa
      601 catggtgaac acggcgatcc cgacgagtac gccacggccc ttgaggtgct tcttcgacag
      661 cacgtaggca taacaggtcg tcagcaccat ggccacggcc gtcgccacga ccgtgtacag
      721 cacggtgttg ccgtagttcc gccagaacat cgagtccttg aacacgatct cgtacgtcgt
      781 gaggttgaac cccttgggca gcagggtcac ctcaccggcc cggatctgcc gttccccgct
      841 gaaggaccgc gcgaggatgt tgacgatggg gtacagggtc accagcacga cgagggtgag
      901 gaccaccccg ttgacgccct ggaagacccg gtaggaacga ctcggcttca ccacaggctc
      961 gtccccactg tgcggcgcga gagctggttg gcggacgtga tcaggatcag gccgatgacc
     1021 gcctcgaaca gtccgatggc ggcggcgtaa ctgaagctgt tggactccac accggaccgg
     1081 tagacgtacg tcgagatcac gtcggcggtc ggatacgtca gcgggttgta cagcagcagg
     1141 accttctcga accgaccgcc atgaacgcgc cgacgttgag gatcagcagc gtgaccatgg
     1201 tgggccggat cccgggcagg gtgacgtgcc agatctgctg ccagtgattg gccccgtcga
     1261 tgcgcgcggc ctcgtacagg tcctcgtcga tggtggtgag cgcggcgagg tagaggatcg
     1321 tcccccagcc ggcggtctgc cagatctccg agccgacgta gatggtgcgg aaccactcgg
     1381 gctcctggat gaaccggatc ggatcgtgcc ccacccgacg gtctgccaca cctccgacga
     1441 cacgtagatc gtgcggaacc actccgggcg ctgcaggaac gggatcggct ccccgccgaa
     1501 cgcgcgcacg agctggttga ccgtgccctc caccgacagc agctgcatga gcatgccggc
     1561 gacgatcacg atggacagga agtgcggcag gtacgacacc gtctgcacga accgctggaa
     1621 ccagcgcacg cgcacctcgt tgagcagcag cgccagcacg atcggcagcg ggaagcagaa
     1681 cagcagggtg agcgcgccga tc
//
LOCUS       DATADN010001059         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_872657, whole genome shotgun sequence.
ACCESSION   DATADN010001059 DATADN010000000
VERSION     DATADN010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872657"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1382
                     /locus_tag="VHH53_13615"
     CDS             303..1382
                     /locus_tag="VHH53_13615"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2291198.1"
                     /translation="MAETLRAALNDLATVAPAWLQELAPRDWYARDGKRIEDARLPRA
                     KAAREAYARTVGEDGFRVLDALASPEAPEDARALPSLTTRRRTWQRHYERTDRTGTTT
                     RQRPAPRVRFRANRDLPRAAEGIESPYDTDARYRHKRDTQWTGYMVHLSETCEPTAPH
                     LITHVHTTAATVHEAQCTAPIQQALVEKALPPSEHLVDAAYIAADLLVQSRDEHRITL
                     RGPTRPSPGWQAHVDGAYTIDQFAVDWAQQRVRCPQGHWSAAWWEHGARESCRPIIVE
                     FAREDCQACPARSVCTRAQQRGRRVRLPPQAQYEAVRAARAWSGSEEGKQQYQQRAGV
                     EGTLSQGVRGFGLRGARYRGLKNTP"
BASE COUNT          279 a          530 c          583 g          244 t
ORIGIN      
        1 gtggtggttg ccgaagcgct tgcggagggt ctgcatggtg gtgaaggtcg agaggcggtc
       61 gaactcggtc cagaggggga gtcgggtacc tgctcggtct ggagctgacc gatccggggt
      121 tcaacttctc cgtgctcagt gagttccgcg accgtttgct ggccgggagc gccgaagcgc
      181 gctgactcga cacgctactg gagcggtgtc gagcgttggg attcctcagg gcccgtgggc
      241 cgcagcgcct ggactccacg cctgtcctcg cggccatccg ggtccgcagc cggctggcat
      301 gggtggctga gaccctccga gcggccctca acgacctcgc cacggtggcc ccggcctggc
      361 tgcaagagct ggcgcctcgc gactggtatg cgcgcgatgg caagcggatc gaagatgcgc
      421 gcctcccccg cgccaaagcc gcccgggagg cgtatgcccg gaccgtgggc gaggacggat
      481 tccgcgtgct ggatgccttg gcgagtcccg aggcgccaga ggacgcacgg gcgttgccca
      541 gcctcacgac acgccgtcgg acctggcagc gccactacga gcggacggat cgcacgggga
      601 ccaccacgcg gcaacgcccg gcgccccgcg tgcgcttcag agccaaccgc gacttaccac
      661 gggccgccga ggggattgaa tcaccctacg acacggacgc gcggtatcgc cataagcggg
      721 acacccagtg gaccggctac atggtgcacc tcagtgaaac gtgtgagccg acggctccgc
      781 acctgatcac gcatgtgcac accacggccg ccaccgtgca tgaggcccag tgcaccgcgc
      841 ccatccagca ggcccttgtg gagaaagcgc tgccgcccag tgagcacctg gttgatgccg
      901 cctacatcgc tgcggacttg ctggtccaga gtcgtgatga gcaccgcatt accttgcgtg
      961 gccccacccg cccgagccca ggctggcagg cgcacgtgga cggggcgtat acgatcgacc
     1021 agttcgcggt ggattgggcc cagcagcggg ttcgctgtcc gcagggccac tggtcggccg
     1081 cttggtggga gcacggcgcc cgggagagct gccgccccat catcgtggag ttcgcccggg
     1141 aggactgtca ggcgtgtccc gcgcgctcgg tctgcacccg ggcgcaacag cggggccgtc
     1201 gcgtgcgact tccaccgcaa gcgcagtacg aggcggtgcg ggcggcgcgg gcgtggagcg
     1261 gaagtgagga gggtaagcaa cagtatcagc agcgagccgg ggtcgaagga acgctctcgc
     1321 agggcgtacg gggcttcggg ttgcggggcg ccaggtatcg gggcctcaag aacacgccct
     1381 gacaacagct cgcgacggcc gccgctatca atgtggatcg actcgtggca tggctcgata
     1441 accgcccgcg ggcgaagacg cggacctcgc gctttgcggc tctggcgcca atctgaccca
     1501 ttcgccaaca gtatccgtag atttaatgag acagaagcag ggtaagcggt cacaggggta
     1561 gtcagggagc acatctcatc acagagcgga ggctgtaggc agtcagctgc acgccgtgat
     1621 taggcgcaga ctcggc
//
LOCUS       DATADN010001060         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_872773, whole genome shotgun sequence.
ACCESSION   DATADN010001060 DATADN010000000
VERSION     DATADN010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872773"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="VHH53_13620"
     CDS             complement(<1..1137)
                     /locus_tag="VHH53_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2291199.1"
                     /translation="MDVVLSLLGLLGMVALTVGTFVCVAAEFSLTALERSTVDAHVQQ
                     VGDRRAHQVQHALRTLSFQLSGAQVGITITTLVTGYIAEPAIARLIRPGLDAVGLPAA
                     TVGSVSLGIALALATALSMLFGELVPKNLAIARPLPTARRVAGLLNAFSTAFGWLIAA
                     LNGSANWIVRRLGIEPAEDLRSARSPQELGSLVRSSAQQGTLDQGTATLLDRSLRFGE
                     RTAEELMTPRVQVQALRADATTLTLVDAARRTGFSRFPVYGSDLDDVRGVVHVKHAFA
                     VPADQRAATPVGALANPVPTVPNSLDGDALLDRLRGQGLQLAIVVDEYGGTAGIVTLE
                     DLVEEIVGDVRDEHDRMELSQVQPLGDDRWIISGLLRVDEVAEAV"
     gene            1334..>1537
                     /locus_tag="VHH53_13625"
     CDS             1334..>1537
                     /locus_tag="VHH53_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291200.1"
                     /translation="MRIGILGGPGLSADVPLDGAVQRLVSDLARRCWGMVLGVPGSVT
                     FDAVESGVDVVEVLARGAEPGTKS"
BASE COUNT          240 a          505 c          555 g          237 t
ORIGIN      
        1 aacggcctcg gccacctcgt cgacccgtag cagcccggag atgatccacc tgtcgtcgcc
       61 tagcggctgg acctgggaca gctccatgcg gtcgtgctcg tcacggacgt cgccgacgat
      121 ctcctccacc agatcctcga gagtcacgat cccggcggtg ccgccgtact cgtcgaccac
      181 gatcgcaagc tgcagtccct ggccgcgcaa ccggtcgagc agtgcatcgc cgtccagcga
      241 gttcggcacg gtgggaaccg gattggccag cgcgccgacc ggggtcgcgg cgcgctggtc
      301 ggccggcact gcgaaggcat gcttgacgtg caccacgcca cgcacgtcgt cgaggtcgct
      361 gccgtagacc gggaagcggg agaatccggt ccgccgcgcg gcgtcgacca gggtcagtgt
      421 ggttgcgtcc gcccgcagtg cctgcacctg cacccggggt gtcatcagct cctccgcggt
      481 gcgctcgccg aagcgcagtg agcggtccag cagcgtcgcg gttccttgat ccagagtgcc
      541 ctgctgggcg ctgctgcgga ccaaggagcc cagttcctgt ggggagcgag cggagcgcag
      601 atcctcggcc ggttcgatgc ccagccgccg cacgatccag ttcgccgagc cgttcaacgc
      661 cgcgatcagc cagccgaacg cggtggagaa cgcgttgagc aggccggcga cccgacgtgc
      721 ggtgggtagc ggccgggcga tggccaggtt cttgggcacg agttcaccga agagcatcga
      781 cagcgcagtg gccagcgcca acgcgattcc cagcgacacg ctccccacgg tggcagcggg
      841 tagcccgaca gcatcgagcc cgggtcggat cagtctggcg atggccggtt cggcgatgta
      901 tccggtgacc agggtggtga tcgtgatacc gacctgcgcc ccggacagct gaaacgacag
      961 tgtgcgcagc gcgtgctgga cctggtgggc gcggcggtcg cctacctgct gcacgtgtgc
     1021 gtcgacggtg ctgcgttcca gggcggtgag ggagaactcg gcggcgacgc acacgaaggt
     1081 gcctacggtc agcgcaacca tcccgagcag gccaagtagc gagagcacaa catccatcta
     1141 tgccgcccag ggggacggcg ggctggggca gccgggccag gagcccggcc ccgtactaca
     1201 gccggggctc gatgcggcag gcaccgaatt cgtcactcct cgaaggccgg atcgcccgag
     1261 tctacgggtg gccggtgcac tgtcgagtgc cgccgcaggc accggacctc cattcggact
     1321 aacgtgtctg atcgtgcgga tcggaatcct gggtgggccc ggactctccg ctgacgtgcc
     1381 gttggatgga gccgtgcagc ggttggtatc cgatctggct cggcgttgct ggggcatggt
     1441 gctcggagtg ccggggtcgg tcaccttcga cgctgtcgag tcgggagtag acgtcgtgga
     1501 ggtgctggcg cgcggcgcgg agccgggtac caagtcc
//
LOCUS       DATADN010001061         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_271821, whole genome shotgun sequence.
ACCESSION   DATADN010001061 DATADN010000000
VERSION     DATADN010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271821"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1392)
                     /locus_tag="VHH53_13630"
     CDS             complement(46..1392)
                     /locus_tag="VHH53_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2291201.1"
                     /translation="MTATSPGHRLDPRSTLAAFLSFLFPGLGQAYNGQRQLAWMLALP
                     ILLLVGIALLAVNAASSGILVRLLDSGFLVGLIVLDLALLGWRVLAIAQAHGWHGPIS
                     PRHWTGWVTGLLLIVTLGMHVLPAYWAVKAIDTLGAVAREGSDGSRGGSSFGRAPGTT
                     PLPEPSHQPNVRLGERVNILLVGIDALPHREGDRLTDSMLVVSLDPDGRSAMISIPRD
                     VYGTPLADGRIYNAKLNSLLSYANDRPEEFPDGGVAALKGAIGELLGIRIHYFAAINL
                     LGFKQAVDAIGGVDIVNRRDIRDPTYFNEFGNRVGFRLSAGPHHLDGSTALAYVRSRK
                     GKGDNDFTRAERQQQVLAAIREKLTAGNLVVSLPGLLDAVKNTISTDVPEDRFPALAA
                     AVQESDVDKLERIVLQPPDYMTADPFSAAGYILIPNLEAIRAVVADMTAARSTAGG"
     gene            1458..>2515
                     /locus_tag="VHH53_13635"
     CDS             1458..>2515
                     /locus_tag="VHH53_13635"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX2291202.1"
                     /translation="MQRPRWLPFSIAPNGPVAVMAGILVLVLVASAVALLGGTRPVAA
                     PPSVTPMPTAPPTSQPTPTPLPTASPTPQPTPTARPQVACPLNGLELASRRMLRRPAL
                     AVQIENHPLARPASNLGSADMVVEATVEGDVTRYTGIYLCNRIRGLVYPVRSGRYYSI
                     DLWQDVRVLPVFFGAGAEAVGRYRAAGMPFVNGISGQWPWFRRGGANPAPHNMHLDIQ
                     GLRRDFATDQRLRRLARRVPDLRPPFRFERGPTPDGRPVKHVEIWTNGFWHFGWDWNP
                     RRDLWERSEAGARHVDRATGRVISARSVVVQLVKEDVVFGPHDPGGYPRRYHHLVGSG
                     RDQRSGMACLLTYNQPRPL"
BASE COUNT          400 a          864 c          880 g          371 t
ORIGIN      
        1 ccggagtcta gcagcggccg gcggcgggat caggaacggg cttggtcagc ctcccgcggt
       61 cgagcgtgcc gcggtcatgt cggccacgac tgcccggatg gcctccaggt tcgggatcag
      121 gatgtagccc gccgcggaga aggggtcagc ggtcatgtag tcaggtggct gtaggacgat
      181 ccgctccagc ttgtcgacgt cggactcctg gacggccgcg gccagcgccg ggaaccggtc
      241 ctcgggaacg tcggtggaga tcgtgttctt gaccgcgtcc agcaggcccg gcagcgacac
      301 cacgaggttg ccggcggtca gcttctcgcg gatcgcggcc agcacctgct gctgccgctc
      361 ggcgcgggtg aagtcattgt cccccttccc cttgcgggag cggacgtatg ccagggccgt
      421 cgagccgtcc agatgatggg gccccgccga gagccggaac ccaacgcggt tgccgaactc
      481 gttgaagtag gtcgggtcac gtatgtcgcg cctgttgacg atatcgacgc cgccgatggc
      541 gtccacggcc tgcttgaagc cgagcaggtt gatggcggcg aagtaatgga tgcggatacc
      601 gagcagctcg ccgatggcgc ccttcagcgc ggcgacgcca ccatcgggga actcctcggg
      661 ccgatcattg gcatacgaca gcagcgagtt gagcttggcg ttgtagatgc ggccatcggc
      721 aagcggcgta ccgtacacgt cgcgcgggat ggagatcatc gccgagcggc cgtccgggtc
      781 gaggctgacc acgagcatgg agtcggtgag ccgatcgccc tcgcggtgcg gcagcgcatc
      841 gatcccgacg agcaggatgt tcacccgctc gccgagccgc acgttcggct ggtgcgatgg
      901 ctcgggcagc ggggtggtgc cgggggcgcg cccgaagctc gagccgcctc gcgagccatc
      961 ggacccctcg cgtgcgaccg cgcccagggt gtcgatggcc ttgaccgccc agtaggctgg
     1021 cagcacgtgc atgccgagcg tcacgatcag cagcaggccg gtcacccatc cggtccagtg
     1081 gcgtggcgag atcggtccgt gccagccatg cgcctgggcg atcgcaagca cccgccagcc
     1141 aagcagggcc aggtccaaca cgatgagccc gaccaggaat ccgctgtcga gcaggcggac
     1201 caggatgccc gagctcgctg cgttgaccgc gagcagggca atgccgacga gcaggaggat
     1261 gggcagcgcg agcatccacg ccagctgacg ctgcccgttg taggcctgcc ccaggccggg
     1321 gaacaggaac gagaggaatg cggccagcgt ggagcgcggg tcgagccgat gtccggggct
     1381 ggtggcggtc acctggagct caccatcccg caatggtgcc acaggccgcc tgacgttgaa
     1441 tcggtagcat ctgttgcatg cagcgacccc gttggcttcc attctcgatc gcgccgaacg
     1501 ggccggtcgc cgtcatggcg gggatcctgg tgctcgtcct cgtcgccagc gcagtggccc
     1561 tgctcggcgg aacccgaccc gtcgccgccc ctcccagcgt cacgcccatg ccgacggccc
     1621 ctcccacgtc ccagccaaca cccacgccgc tgcccacggc cagccccaca ccccagccga
     1681 cgccaacggc ccgccctcag gtggcctgtc cgctcaacgg cctcgagctg gcgagccggc
     1741 gcatgctgcg ccgtccggct ctggccgtcc agatcgagaa ccacccgctc gcacgtccgg
     1801 cgagcaacct cggcagcgcc gacatggtag tcgaggccac cgtcgagggc gatgtgacgc
     1861 gctacacggg catctacctc tgcaaccgaa tccgtgggct ggtctacccg gtgcggtccg
     1921 ggcgctacta cagcatcgac ctgtggcagg acgtgcgcgt gctgccggtc ttcttcgggg
     1981 ccggtgccga ggcggtcggg cgctaccgcg cagcaggcat gccgttcgtg aacggcatct
     2041 ccggacagtg gccgtggttc aggcgtggcg gcgcgaaccc ggcgccacac aacatgcacc
     2101 tggacatcca gggccttcga cgagatttcg ccacggacca gcggctgcga cgcctcgcac
     2161 gccgggtgcc cgacctacgg ccgccattcc gcttcgagcg cggaccgacg cccgacggga
     2221 ggccggtaaa gcacgtcgag atctggacca acggcttctg gcacttcgga tgggactgga
     2281 acccgcggcg cgatctgtgg gagcgttccg aggcgggcgc gaggcatgtc gatcgggcca
     2341 ccggcagggt gatcagcgcc cgctcggtcg tcgtccagct cgtcaaggag gacgtggtct
     2401 tcgggccgca cgatcccggc ggctatccga ggcgctacca ccacctggta ggaagtggcc
     2461 gcgaccagcg cagcgggatg gcctgcttgt tgacgtacaa ccagccgcgg ccact
//
LOCUS       DATADN010001062         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1493846, whole genome shotgun sequence.
ACCESSION   DATADN010001062 DATADN010000000
VERSION     DATADN010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493846"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1753
                     /locus_tag="VHH53_13640"
     CDS             311..1753
                     /locus_tag="VHH53_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX2291203.1"
                     /translation="MGLEPAQARTTLPSKYLPPVEFRDLPEPVPLRKVTGASVIILAT
                     AIGSGEIVLWPYITTQVGFTFMWAAVVGFAIQFFLNMEIERYTLATGESAITGFARFW
                     KPWWWLFIIFALCQNFWPGWATGASTALSFALGLGENAPIVPITIAALIAIGITLTLS
                     PVVYQAVKKIQFVLVALIMLFVVVAIPVATTGEAWGALFTDGVGFPVGQGNLDIALLL
                     GALAFAGAGGANNLVQSNYIRDKGMGMGAHMPKIVSPITGHEEAKPSLGYMFPTDAEN
                     MRRWRGWWKVANWEQFITFFLVGVISLIIMAVLAYSTLPVGQVEAQNLDFLRLEGQTL
                     SETVAPWFGTAFWFTAVITLFSTNLGILDYTSRLIADQLKINALKDSTVWTESRIYAT
                     TVWLMIIVGSLILLSGVSQPFLLLVISSSIAGVQMFIYSGLLIQLNRKALPKELQIGG
                     VRLAVLAVSFLFFGFLSVLLVLDNLGVSIG"
BASE COUNT          289 a          636 c          610 g          370 t
ORIGIN      
        1 gtcgtcggtc gtccactacc gcgcgttgtt cggccacgcc acgaccaccg accgcgaggt
       61 cgagcaggcg gtcgaggccc tgctgtccgg tatcgccgtc gactacccgc ggctggtcgc
      121 ccacagccgc gagctgaccg aggcgcatca cgcacatgcg tgacggcttg atcctctcgg
      181 ctggcgggac ggaccttgac agcgcatacc gtatgctgct gcgatcaacc ggcccggtca
      241 cccgtccggg tgagtagctg gtgcacgcgg tggcgcgtcg gcggccaccc tgacgcgcaa
      301 ggaggcgccg atgggcctcg agcccgcgca agcccggacc acacttccga gcaagtacct
      361 gccaccggtc gagttcagag acctaccgga accggtaccg ctgcggaagg tgaccggggc
      421 gagcgtcatc atcctggcca ccgccatcgg gtccggggag atcgtcctct ggccctacat
      481 caccacgcag gtcggcttca ccttcatgtg ggccgcggtc gtcggattcg cgatccagtt
      541 cttcctcaac atggagatcg agcgctacac gctcgccacc ggggagtccg cgatcaccgg
      601 cttcgcgcgg ttctggaagc cgtggtggtg gctgttcatc atcttcgcgc tctgccagaa
      661 cttctggccc ggctgggcga ccggcgcgtc gacggccctg tcgttcgcgc tcgggctggg
      721 tgagaacgca ccgatcgttc cgatcaccat cgcggcgctc atcgccatcg ggatcacgct
      781 gaccctctcc cccgtcgtct accaggccgt caagaagatc cagttcgtgc tggtggcgct
      841 gatcatgctc ttcgtcgtcg tcgcgatccc ggtggcaacg acgggcgagg catggggcgc
      901 gctcttcacc gacggcgtcg gtttcccggt cgggcaaggg aacctggaca tcgcactgct
      961 cctcggcgcg ctggccttcg ccggcgcggg cggtgcgaac aacctcgtgc agagcaacta
     1021 catccgggac aagggcatgg ggatgggcgc gcacatgccc aagatcgtct cccccatcac
     1081 gggacacgag gaggcgaagc cctcactcgg gtacatgttc ccgaccgatg ccgagaacat
     1141 gcggcggtgg cgcggctggt ggaaggtcgc caactgggag cagttcatca ccttcttcct
     1201 cgtcggggtc atctcgctca tcatcatggc ggtgctggcg tactcgacgc tgccggtcgg
     1261 ccaggtggaa gcgcagaacc tcgacttcct gcgtctggag gggcagaccc tttcggagac
     1321 cgtggcgccg tggttcggca ccgccttctg gttcaccgcg gtcatcaccc tgttctcgac
     1381 caacctgggg atcctcgact acacaagccg cctgatcgcc gaccagctca agatcaacgc
     1441 gctgaaggac agcaccgtct ggaccgagag caggatctac gccaccaccg tctggctcat
     1501 gatcatcgtg ggctcgctga tcctgctctc gggtgtcagc caacccttcc tgctgctggt
     1561 gatctcgtcc tcgatcgcgg gcgtgcagat gttcatctac tccgggctgc tgatccagct
     1621 caaccggaag gcgttgccga aggagctgca gatcggaggg gtgcggctcg cggtcctggc
     1681 cgtctcgttc ctcttcttcg ggttcttgtc ggtgctgctc gttctcgaca acctcggcgt
     1741 cagcatcggc tgatccgttg ctcaaacggc tgtggctgcg cattttggtg gcgctggtga
     1801 tctggatggt gctgctggcc ctcatgtggg ccgtcggcat catcggttga tctcgtgaac
     1861 agctgatctc tcagcgtcgg ttcagccatc cggtcgcagt gtggg
//
LOCUS       DATADN010001063         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_873789, whole genome shotgun sequence.
ACCESSION   DATADN010001063 DATADN010000000
VERSION     DATADN010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873789"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VHH53_13645"
     CDS             complement(<1..404)
                     /locus_tag="VHH53_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX2291204.1"
                     /translation="MILVAGLVVALGALVQGAVGYGMALVAAPMLALFDPALVPVPLI
                     LLMSVHSVLAVVRDGRHADWPGIGWAMLGRLPGTGLGVLAVVMLSQRAFSLIVGLCVL
                     ACVVLSLLTWRPRPRPRALVIAGIASGAAGTAA"
     gene            complement(463..1239)
                     /locus_tag="VHH53_13650"
     CDS             complement(463..1239)
                     /locus_tag="VHH53_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2291205.1"
                     /translation="MSLILVTGGTGVLGRAVVRHLLDSGHQVRVASRRPPPAGPRPYA
                     WTTVDYRSGEGLDTALHAADSVIHCAGDFRGEVDRALLTALRAAGGPHLVYISIVGVD
                     RIPLAYYRLKLAAEQAIENSDVPYTILRTTQFHDLIRCMLAATARLPVMLVPDLSVQP
                     IAVRDVAVRLVELAVRRPSGHARDLGGPQVRTVRDLAQIYLRATGRRRLLLPVRLPGE
                     VFHGYRHGGHLTPHHAEGSITFEKYLTTQPAPTRISYRGS"
     gene            complement(1330..>1843)
                     /locus_tag="VHH53_13655"
     CDS             complement(1330..>1843)
                     /locus_tag="VHH53_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2291206.1"
                     /translation="SPAKAPFDRLHCAVLAELGGGTCRIGVGGVCERPNEFPRSYREA
                     QFALKMQVVAKGATQVVGFESLGVYRLLSEVQDTAGVDRFVRSWLGALLDYDSRKHSA
                     LVDTLSEYLECGGSYDAAAASLSVHRNTLKYRLQRIRELSGHDLSCPDTRFNLQLATR
                     AWQTLVGLRG"
BASE COUNT          350 a          631 c          609 g          253 t
ORIGIN      
        1 gccgcggtcc ctgcggcccc gctggcgatt ccggcgatca ccagcgcacg gggccgggga
       61 cgcggccgcc aggtgagcag cgacagcacc acgcacgcca gcacgcagag tccgacgatc
      121 aagctgaacg cccgctgtga cagcatcacc acagcgagta ctcccagccc ggtgccgggc
      181 agccggccca gcatcgccca cccgatcccg ggccagtcgg cgtggcgccc atcccgaaca
      241 actgcgagca ccgagtgcac gctcatcagc aagatcaacg gaaccgggac gagcgcggga
      301 tcgaacaggg ccagcatcgg tgccgccacc aaggccatgc cgtatccgac cgcgccctgc
      361 accaacgcac ccagcgccac caccagaccg gcgacaagga tcatcaccca cagcctggcg
      421 agccggacgc agcgctatgg agctggaccg tgtctccggt catcagctac cccggtagga
      481 gatccgtgtt ggtgccggct gggtcgtcag atatttctcg aaagtgatgc tgccctcggc
      541 gtgatgtggc gtgagatgcc cgccgtggcg gtagccgtgg aacacctcgc cgggcagccg
      601 gacgggcagt agcagacggc gacgcccggt ggcccgcagg tatatctgcg ccaggtcccg
      661 gaccgttcgc acctgaggac cgccaagatc gcgagcgtgg ccgctcggtc ggcggacggc
      721 aagctcgacg agacggaccg cgacgtcacg aacggcgatc ggttggacgc tgaggtcggg
      781 caccagcatc accgggaggc gtgcggtggc ggcgagcatg caccggatca ggtcgtggaa
      841 ctgggtggta cgcaggatgg tgtagggcac atcggagttc tcgattgcct gttctgcagc
      901 gagcttgagc cggtagtagg cgagtgggat gcgatcgacc cccacaatgg agatgtacac
      961 cagatgcggc ccgccggctg cccgcagagc cgtgagcagt gcccgatcca cctcgccgcg
     1021 aaagtcgcct gcgcagtgga taaccgagtc cgccgcgtgc agcgcggtgt cgaggccctc
     1081 gccgctgcgg taatcgacgg ttgtccaagc gtagggccgc ggacccgccg gcggcggcct
     1141 ccggctcgcg acccggacct ggtgcccaga gtcgagcaag tgtcgcacca cggcacgacc
     1201 gaggacgccg gtgccgccgg tgacaaggat caagctcatc gcggttccgt tcgtccgagc
     1261 gtgtgcctgt caccagctat ggcggggtag acccccgcat gtaacaagcg cttgtcaccg
     1321 catgcaacat caaccgcgta aacccaccag ggtctgccag gcgcgggtgg ccaactgcag
     1381 gttgaagcgg gtatccggac aggacagatc atggccggac agttcccgga tccgctgcag
     1441 ccggtacttc aatgtattgc ggtgcaccga caacgacgcc gcggcggcat cgtagctccc
     1501 gccgcactcc aggtactcgg acaacgtgtc caccaaggcg gaatgcttgc gggaatcgta
     1561 atcgaggagg gcacccaacc acgaccggac gaaccggtcg acacccgcag tgtcctggac
     1621 ctccgacagc agccggtaca caccgaggct ctcgaatccg acgacctggg tcgcaccttt
     1681 cgccacgacc tgcatcttca gcgcgaactg ggcttcccgg taggagcgag ggaactcgtt
     1741 tggtcgctcg catactccgc cgaccccgat ccggcacgtg ccgccgccga gctccgccaa
     1801 gacggcacaa tgcagccgat cgaacggcgc cttggcaggg gaa
//
LOCUS       DATADN010001064         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1494453, whole genome shotgun sequence.
ACCESSION   DATADN010001064 DATADN010000000
VERSION     DATADN010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494453"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VHH53_13660"
     CDS             complement(<1..247)
                     /locus_tag="VHH53_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291207.1"
                     /translation="MLAGVVLGNGVHCAGGMTAMAIEDAASSRMPAGTPGAFTMGAQR
                     PPVCPGCRHLRPRGHCGGTVNFVVCVAGDDRRAASTND"
     gene            918..1103
                     /locus_tag="VHH53_13665"
     CDS             918..1103
                     /locus_tag="VHH53_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2291208.1"
                     /translation="MAEEYLTIPEVVAELRVPRSTFYYWRQLGRGPKALKLPNGDVRI
                     RRRDLDSWLCDLEECVR"
     gene            1205..1462
                     /locus_tag="VHH53_13670"
     CDS             1205..1462
                     /locus_tag="VHH53_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291209.1"
                     /translation="MTRALAESFRAELMSAARTGEAFDEVTGLPESRARDVPWFDHAV
                     SYVDTKWPRAAAKSRKSIAEALTTVTMALLLPPDAAAPTMP"
     gene            1552..2538
                     /locus_tag="VHH53_13675"
     CDS             1552..2538
                     /locus_tag="VHH53_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2291210.1"
                     /translation="MSHASRPVSSLRDLAVIRSVIDALTVRLDGKPAAATSVYRKRAV
                     FYNALGLAVERRLLLSNPVDLVQWTVPDVSESVDRRVVASPDQLRALLDAVRGQPRRG
                     EYLVAFFGCLYYAGMRPAEVVALRAGDCELPASGWGRLQLARSEPWAGQEWTDNGEAR
                     ERRALKRRAPNTVRTVPIPAELVGLLRAHLGTFGTGDAGGVFHNERGGPFNEASARRA
                     WARARLSTLTQEQVHSPLARRPYDLRHAAASLWLNNGVPATEVARRLGHDVAVLLKIY
                     ANCIDGQEGEINDRITAALTANHNHETLPPAPAVDTAAQPNPRTRRGPEPAQ"
     gene            2567..2642
                     /locus_tag="VHH53_13680"
     tRNA            2567..2642
                     /locus_tag="VHH53_13680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2600..2602,aa:Arg,seq:cct)
BASE COUNT          530 a          977 c          890 g          448 t
ORIGIN      
        1 cgtcgttggt cgacgcggcg cgtcgatcat ccccagctac gcagaccacg aagttgacag
       61 tgccaccaca gtgacctcgg gggcgcaggt gacgacatcc gggacagaca gggggacgtt
      121 gtgcccccat cgtgaaggct ccgggcgtgc cagccggcat gcgagaactt gcggcgtcct
      181 cgatggccat cgcggtcatg ccgccggcgc agtgcacacc gttcccgagc accacgccag
      241 ctagcatgcc gaccaccaca gccagccgcg caagatagcc acccgcggct cacgcacggc
      301 gagtcctccc cctcacaccg atcactgtaa gacccacgca cccgatcgtg atctacttga
      361 tccatcgatt cgccgcgtcg ctcacttctg ccccgcgagg tcgaagtcgg cgaacaacgc
      421 gtgcgtatag gcatttggtt caagtccccc cacggacttg aacccccacg gctagctcgt
      481 tgagcccacc accgccaacg cagatccacc accctgccca tcccctcgcg gttgcccgtg
      541 tcagcccctt accgtgcagc aggctgctcg taggcatgct cggatcgcgc tcgatcacag
      601 gcgtgggcat tactacctat gacgaggagt tgcggtcact tcacgtcaaa aagccgccgg
      661 tactccaccg ccctgggcgc gatccgcggt gaacgccacg cctaccgaca acgccaagcc
      721 gccgaacaca tccgcgaact ggcacccgag gagcacgaca ccacccttgt cgtcgcccgc
      781 tgggagttgc cggcctcggt tacctcacca ccggagacac cgcactggcc ctctccgccg
      841 ccgcccgagc acgcgaacga cgacaggcgg cacggtagcg gcgtaggtcc acggcaagaa
      901 acgcgatagg agcgacggtg gcagaggaat atctgaccat tcctgaggtc gttgccgagc
      961 tgcgcgtgcc tcgatcgact ttttactact ggcgccagct cggacgtggg ccgaaagcgc
     1021 tcaagctgcc caacggtgat gttcgtattc ggcgtcgtga cctagattcc tggttgtgtg
     1081 atctggagga gtgtgtgagg tgagtagcta tgagttacgg atgtggggca tccggaaacg
     1141 tgatcgacag aaaccgtatc aagtcagatg gttagtcgcc ggccgctcgc acgcggagac
     1201 ctttgtgacg cgcgcgctgg ccgagtcttt ccgcgctgaa ctcatgtcag ccgcccgtac
     1261 cggagaagcg ttcgacgaag tcactgggct gccggaatcc agggcacggg atgtgccgtg
     1321 gttcgaccat gcggtctcct atgtagatac caagtggcca agggcagcgg cgaagtcgcg
     1381 caagtccatc gcggaagcgt tgaccacggt gaccatggcg ctgctactcc cccccgacgc
     1441 ggccgccccg acgatgccct gatccgccgc gcgctgttcg gatgggcatt caacaccggg
     1501 cgccgaacca ctggcccgcc acccaccgag atcgccgagg cgctggcctg ggtgtctcac
     1561 gcgtctcggc cggtgtcctc gctgcgcgat ctggcggtga ttcgcagtgt gatcgatgcg
     1621 ctcacggtgc gtctggatgg caagcccgcg gcggcgacca gcgtctaccg caagcgcgcg
     1681 gtgttctaca acgctctcgg gctcgccgtc gagcggcggt tgttgctgag caacccggtt
     1741 gacctggtgc aatggacggt gcccgatgtg tcggaatcgg tggaccgccg ggtcgtggcc
     1801 agcccagacc agctccgcgc gctgctcgat gccgtgcggg gccaacctcg ccggggcgag
     1861 tacctggtgg ccttcttcgg gtgcctgtac tacgcgggca tgcgaccggc cgaagtcgtc
     1921 gccctacgag ctggagactg cgagctgccg gcgtccggct ggggacggct ccaacttgcc
     1981 cggtcagaac cctgggccgg tcaggaatgg accgacaacg gtgaagctcg cgaacgccgc
     2041 gcgctcaagc gccgcgcacc caacacggtc aggaccgtgc caatccccgc cgagctagtc
     2101 ggcctgctcc gcgcacacct gggaaccttc gggaccgggg acgccggagg ggtgttccac
     2161 aacgagcgcg gtggaccgtt caacgaggcc agcgcccggc gagcctgggc cagagcacga
     2221 ctctcgacac tgacacaaga acaggtccac tcaccactgg cgcgccgccc ctacgacctg
     2281 cgccacgccg cagcctcgct ctggctcaac aacggcgtcc ccgccaccga ggtcgcccgc
     2341 cggctcggcc acgacgtagc cgtactgctc aagatctacg ccaactgcat cgacggacag
     2401 gaaggggaga tcaatgaccg gatcaccgcc gctctcacgg ccaaccacaa ccatgagaca
     2461 ctgcccccgg cgccagccgt ggacactgcg gcccagccaa acccgcggac cagacgcgga
     2521 ccagagccag cacaataggc tccggacccg gaaagaccgc aggtcagccc tcgtagctca
     2581 ggggatagag catcggtttc ctaaaccgtg tgtcgcaggt tcgaatcctg ccgggggcac
     2641 cacgattgaa caggtaaaat gcgcaattaa gcataacgct tggggcgctc atgacactgg
     2701 ttatgacgca agccggctat gctggcgtca tgacacgggt agctggtcgg cagaggcgac
     2761 agcggggaag catcgacgag cttcccagtg gtgcgctgcg cgtccgcgtc tatgccgggg
     2821 ttgaccctgt cacgaggcgt cggca
//
LOCUS       DATADN010001065         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_588049, whole genome shotgun sequence.
ACCESSION   DATADN010001065 DATADN010000000
VERSION     DATADN010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588049"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VHH53_13685"
     CDS             <1..200
                     /locus_tag="VHH53_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HEX2291211.1"
                     /translation="AVADSRQTLLCAYLSGALLAGLLLNSLFGWSWADPIVALIIAGV
                     AVREGQQAWRGKHCCGAVNGC"
     gene            190..1341
                     /locus_tag="VHH53_13690"
     CDS             190..1341
                     /locus_tag="VHH53_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2291212.1"
                     /translation="MAADSLRAAIETLPLVDHHVHGAATDDVDGGGFEAGLTESPWPS
                     PAGSTHLDSQVGFAVRRWCAPVLDLDPHVDVGTYLARRRELGAQEVTRRLLAAGGIEW
                     FLVDTGYRADNALGPLEMAATCGAAAAEIVRLEAVAEDVAASGVDAAGFADRFASTLA
                     ERARHAVGLKSVIAYRYGLDFDPEPPSAGEVRRAASHWLPRWQTGELRVTDPVLLRHL
                     LWVGLEHRLPLQFHVGYGDPDLTLHRCNPSLMTEFLRRTRDRHVPVMLLHCYPYHRTA
                     GYLAHVFPHVYLDVGLAVNYTGARSRAIVAESLEVAPFGKVLLSSDAWGPAELHYLGT
                     VLWRRAMSSVLSEWVESGEWSGADAQRVAQMVGAGNAKRVYRLEEWGHS"
     gene            1338..>1784
                     /locus_tag="VHH53_13695"
     CDS             1338..>1784
                     /locus_tag="VHH53_13695"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2291213.1"
                     /translation="MIVAMAGLPGVGKSTLAGALATRLGAAVLDKDRIRAGLFPPSHV
                     DYTDDQNDFCVDVMYRTAEWLLRRDPASVVILDGCTYTRSHQIAGLRQRAADLGEPLW
                     LIECACNDPLALARIADDGTHLAGNRNAALYRRLQAAAHAITGPKLV"
BASE COUNT          248 a          587 c          613 g          336 t
ORIGIN      
        1 gcgcggtggc tgattcccgg cagaccctgc tgtgtgccta cctgtccggg gcgctactgg
       61 ccgggttgct cctcaacagc ctgttcggct ggtcgtgggc ggatccgatc gttgctctga
      121 tcattgctgg cgtggcggtc agggaaggtc agcaggcctg gcggggcaag cattgctgcg
      181 gagccgttaa tggctgctga ttcgctgcgt gcggccatcg agaccctgcc tctggtggat
      241 caccacgtgc acggtgccgc gacggatgac gtcgacggtg gcggattcga ggccggcctc
      301 actgagtctc cctggccgtc acctgcggga agtacgcatt tggattcgca ggtgggcttc
      361 gcggtccgtc gctggtgcgc cccggtgctc gacctggacc cgcacgtgga cgtcggtacc
      421 tacctggctc ggcgccgcga gctcggtgcc caagaggtca cccgtcggct gctcgctgca
      481 ggtggcatcg agtggttcct ggtcgacacc ggctaccgcg cggacaacgc cctgggaccg
      541 ctcgagatgg cggccacctg tggagcggcg gcagcggaga tcgttcgcct cgaggcggtc
      601 gccgaggacg tggcggctag tggggtggac gctgccgggt tcgccgaccg gttcgcctcg
      661 accctggccg agcgggcccg gcacgccgtg gggcttaaat ccgtcatcgc ttaccggtac
      721 ggcctcgact tcgatcccga accacccagt gccggggagg tacgccgtgc tgccagccac
      781 tggctaccgc gctggcagac cggtgagctg cgtgtgactg atccggtgct gctgcgccat
      841 ctgctctggg taggcctcga acatcgccta ccgctgcagt ttcatgtcgg gtacggggac
      901 cctgacctga ccttgcaccg ctgcaacccc tcgctcatga ccgaatttct gcgccggaca
      961 cgggaccgcc acgttccggt gatgctgttg cactgctatc cgtatcaccg cacggccggg
     1021 tatctggctc acgtgtttcc gcacgtgtac ctcgatgtcg gattggcggt gaattacacc
     1081 ggggcgcgtt cccgtgccat cgtcgccgaa tcgctggaag tggcgccctt cggcaaggtg
     1141 ctgttatcct cggatgcgtg gggtcccgcc gagctccatt atctcggaac cgtgctgtgg
     1201 cggcgtgcca tgagcagcgt cctcagcgag tgggtggaat cgggggagtg gtcaggtgcc
     1261 gacgcgcagc gcgtcgccca gatggtcggc gcgggcaacg cgaaacgcgt ctaccggctg
     1321 gaggagtggg ggcattcgtg atcgtcgcca tggccggctt gcccggcgtc ggcaaaagta
     1381 ccttggcggg cgctctcgcg acccggcttg gtgcggccgt gctggacaag gaccgcatcc
     1441 gcgctgggct gttcccgccg tcgcatgtgg actacaccga cgaccagaat gatttctgcg
     1501 tcgacgtcat gtaccgcacc gctgagtggc tgttgcgccg cgaccctgcc tcggtcgtca
     1561 tcctcgatgg ctgcacctat acccgctcgc accagatcgc cgggcttcgc cagagggcgg
     1621 cggatctggg cgaaccgttg tggctcatcg aatgtgcctg caacgacccg ctggcgttgg
     1681 cgcgcatcgc cgacgacggc actcacctcg ccggcaaccg caacgcagcg ctgtaccgca
     1741 ggctgcaagc tgcggcacac gcgatcaccg gccccaagct cgtc
//
LOCUS       DATADN010001066         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_588211, whole genome shotgun sequence.
ACCESSION   DATADN010001066 DATADN010000000
VERSION     DATADN010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588211"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VHH53_13700"
     CDS             <1..521
                     /locus_tag="VHH53_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009938697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2291214.1"
                     /translation="AVAGNETTRNSITHGMIAFSQNPDQWELYKRERPETAADEIVRW
                     ATPVSAFQRTALEDVELGGVQIKKGQRVVMSYRSANFDDEVFENPHKFDIMRNPNPHV
                     GFGGTGAHYCIGANLAKMTINLIFNAVADHMPDLKPIGEPERLKSGWLNGIKHWQVDY
                     TGTCPVAHEPTK"
     gene            565..>1555
                     /locus_tag="VHH53_13705"
     CDS             565..>1555
                     /locus_tag="VHH53_13705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2291215.1"
                     /translation="MDFSPDEGQQAVADVVTSVLSRDNTWEALVFGGVTALAVPERLG
                     GDGVGLLEVSTALTEIGRHGTVSPALATLGLGVLPLLELASDEQQDRYLAEVAKGSVV
                     TAALNEPGAALPDRPSVALTDNKVTGAKIAVPYAGQADWLIVTTGNAVVVVSAKADGV
                     QITKTPTSNGSDEYSVRFTDVEVDGVLEGAQPRRVNQLALAAIGAFASGLVAGALRLT
                     ADYVANRHQFGKPLSTFQTVAAQLAEVYIASRTLTLVANSVSWRLAEGLDADDDLDIL
                     GYWLASQAPPVMQLCHHLHGGMGMDITYPMDRYCSSIKDLTRLVGGRSHRLELV"
BASE COUNT          277 a          504 c          514 g          260 t
ORIGIN      
        1 tcgcggtggc gggcaacgag accacccgca actcgatcac ccacggcatg atcgcgttct
       61 cacagaatcc cgatcagtgg gagttgtaca agcgggaacg gcccgagacc gccgccgacg
      121 agatcgtccg ctgggccact ccggtttcgg ccttccagcg caccgcgctg gaggatgtcg
      181 agctgggcgg cgtgcagatc aagaaggggc agcgggtcgt gatgtcctac cgctcggcca
      241 acttcgacga cgaggtattc gagaacccgc acaagttcga catcatgcgc aaccccaatc
      301 cccacgtcgg attcggcgga accggcgccc actactgcat cggcgcgaac ctggcgaaga
      361 tgaccatcaa tctgatcttc aacgcggtgg ccgatcacat gcccgacctc aagccgatcg
      421 gtgaaccgga gcggctcaag tccggctggc tcaacggcat caagcactgg caggtggatt
      481 acaccggcac gtgcccggtg gcccacgaac cgaccaagta gctttagcca cggcggaaat
      541 ttcccggtat caaggaggat tcgggtggac ttcagtccag acgaagggca gcaggctgtc
      601 gccgacgtgg tcacttcggt gctcagccgc gacaacactt gggaagccct ggttttcggc
      661 ggtgtcaccg cgttggccgt acccgagcgt ctcggcggag acggggtggg cctcctcgag
      721 gtgtcgaccg cgctgaccga gatcggccga cacggcaccg tcagccccgc cctggccacc
      781 ctcggtctgg gcgtgctgcc gcttctcgaa ctagcctccg acgaacagca ggaccgttac
      841 ctcgccgagg tagcgaaggg gtcggtggtg acggcggcgc tcaatgaacc gggcgctgcg
      901 ctgccggacc ggccttcggt cgcgttgacc gacaacaaag tcaccggcgc caagatcgcg
      961 gttccctatg cggggcaggc ggattggctg atcgtgacga ccggcaacgc ggtcgtggtg
     1021 gtgtctgcca aggccgacgg cgtgcagatc accaagacgc cgacgtccaa tggctcggac
     1081 gaatactccg tcaggttcac cgacgtcgag gtcgacggcg tgctcgaggg tgcgcagccc
     1141 cgccgcgtca accagctggc gttggccgcc atcggcgcgt tcgcctccgg gttggtcgcc
     1201 ggggcgttgc ggctgaccgc cgactatgtg gcaaaccgcc accagttcgg taagccgctg
     1261 tcgacgttcc agacggtcgc ggcgcagcta gcggaggtct acatcgcctc gcggacattg
     1321 actttggtgg ccaactcggt gtcatggcga ctggctgaag gtctcgacgc agacgacgat
     1381 ctcgatatcc tcggctactg gctggcctcg caggcgccgc cggtgatgca gctctgccac
     1441 cacctgcacg gcgggatggg catggacatc acgtacccga tggatcggta ctgctcctcg
     1501 atcaaggact tgacccgcct ggtgggcggt cggtcgcacc ggctcgagtt ggtgg
//
LOCUS       DATADN010001067         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1494772, whole genome shotgun sequence.
ACCESSION   DATADN010001067 DATADN010000000
VERSION     DATADN010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494772"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VHH53_13710"
     CDS             complement(<1..855)
                     /locus_tag="VHH53_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX2291216.1"
                     /translation="MTPLELELTVGPVAHGGFCVARHEGRVVFVRHALPGERVRAVVT
                     EDHGGSFCRADAVAVLEPVVGRVPAPCPVSGPGGCGGCDFQHADPRLQRQLKGQVVAE
                     QLRRIAKLDVAVTVQELPGGTLGWRTRTRLAVDDAGRAGFRAHRSHTVLGVPACPLAV
                     PGSLDLVLSRRWRPGSELEVVADDSGQHVAEFRAGRPPTQRRGTGRVARHAAGRRWEL
                     SAHGFWQVHPAAADALAALVGQWAVAPLGGTAWDLYGGVGLFASVLAGQVGPTGSVTV
                     VETSRRAAA"
     gene            complement(852..>2443)
                     /locus_tag="VHH53_13715"
     CDS             complement(852..>2443)
                     /locus_tag="VHH53_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2291217.1"
                     /translation="IPTYAFMIGIFAMLAWGFIRVLLFDEQLRAESAEFGLHVEGDQL
                     VGFALMFLVLRAFSSGCAALTGVEAISNGVPAFQKPKSRNAATTLLLLGSISVTMLMG
                     LILLAQFAGVKMAEDPAGQLVGAPAGYEQKTMVAQIAQAVFDGFPAAFYFITVVTALI
                     LVLAANTAFNGFPVLGSILAQDRYLPRQLHTRGDRLAFSNGIVFLAGGAIVLVFAFDA
                     EVTQLIHLYIVGVFVSFTLSQTGMVRHWNRLLATGPDPAQRRRIRRSQAINVLGLVMT
                     GSVLVVVLVTKFVEGAWIAITAMGVIFLLMQAIRRHYDRVVAELVPQEADTVLPSRNH
                     AIVLVSTMHLPTLRALSYARATRPDVLEAVTVNVDDVSTRALVAEWEQRGLPVPLKVV
                     ESPYREITKPVIDYVKRARSDNPRNVVTVFIPEYVVGHWWEQILHNQSALRIKGRLLF
                     EPRVMVTSVPWQLRSSARVVEREQLAPGAVRWGLGTGPAGTSGNATPVDGVPAADGGT
                     AGDTAVGEMATKPQPRQVDRP"
BASE COUNT          448 a          877 c          792 g          326 t
ORIGIN      
        1 ggccgcggcg cgccgggagg tctccacgac cgtcaccgag ccggtcggcc cgacctgacc
       61 ggcgagaacc gaggcgaaca gccctacccc gccatagagg tcccaggccg tgcctcccag
      121 cggggccacc gcccactggc cgaccagtgc cgccagcgca tcggccgctg cggggtgcac
      181 ctgccaaaag ccgtgcgcgg acagctccca gcgtcgaccg gcagcatgcc gtgccacgcg
      241 tccagtgccg cgacgctgtg tgggcggccg gccggcccga aactccgcta catgctgccc
      301 ggaatcgtcg gcgaccacct ctagctcgct gcccgggcgc cagcgacgcg acaacaccag
      361 atccagggag ccgggaaccg ccagcgggca ggccggcacc cccagcaccg tatggctgcg
      421 atgcgcccgg aaaccggctc gcccggcgtc gtccaccgcc agccgggtcc gggtccgcca
      481 gcccaacgtg ccaccgggca gctcctgcac cgtcaccgcg acatccagtt tggcgatccg
      541 gcgcagctgc tcggctacta cctgcccttt gagctggcgc tgcagccgtg gatcggcatg
      601 ttggaagtcg cagccaccac aaccgccggg tcccgaaacc ggacagggcg ccggcacccg
      661 gccgaccacc ggttcgagca ctgccaccgc gtcggcccgg cagaaggacc cgccgtggtc
      721 ctcggtgacc accgcgcgaa cccgctcccc cggcagcgcg tgccggacga acaccactcg
      781 accctcgtgg cgggccacgc agaacccacc atgagccacc ggtccgacgg tgagctccag
      841 ttcgagggga gtcacggccg gtcgacctgc cgtggctgtg gcttggttgc catctcgcca
      901 accgctgtat cgccggcggt cccgccgtct gcagctggga caccatcgac tggcgtggcg
      961 ttccctgagg tgcccgcggg gccggtgccc aagccccagc gcaccgctcc gggggccagc
     1021 tgctcacgtt cgacaacgcg ggccgaggaa cgcagctgcc agggcacgct ggtcaccatc
     1081 acccgaggct cgaacagtag ccggcccttg atccgcagcg cgctctggtt gtgcaggatc
     1141 tgctcccacc agtggcccac gacgtattcc gggatgaaca ccgtgaccac gttacggggg
     1201 ttatccgagc gcgcccgctt gacgtagtcg atcaccggct tggtgatctc ccggtagggc
     1261 gactcgacca ccttgagtgg aaccgggagc ccacgctgct cccattccgc gaccagggct
     1321 cgagtgctca cgtcatcgac gttgacggtg accgcctcca gcacgtcagg acgggtggcg
     1381 cgggcgtagg acagcgcccg cagggtcggc aggtgcatgg tggacaccag cacgatcgcg
     1441 tgattacgcg agggcagcac ggtgtcggcc tcctgcggca ccaactcagc gaccacccgg
     1501 tcgtagtgcc tgcggatcgc ctgcatcaac aggaagatca cccccatggc cgtgatcgcg
     1561 atccaggcgc cctcaacgaa cttggtgacc agcaccacca ccagcaccga gccggtcatc
     1621 accaggccga gtacgttgat cgcctgagaa cgtcggatcc gccgacgctg cgctggatcg
     1681 ggcccagtgg cgagcagccg gttccagtgc cgcaccatgc cggtttggct cagcgtgaat
     1741 gagacgaaca ccccgacgat gtagaggtgg atcagctggg tgacctcagc gtcgaacgca
     1801 aacaccaaga cgatcgcccc gccagccagg aagacaatac cgttggagaa ggccaaccga
     1861 tcgccacggg tgtgcagctg ccgaggcagg tagcggtcct gagccaggat cgagcccaac
     1921 accgggaagc cgttgaacgc ggtgttcgca gccagcacca ggatcagcgc ggtgaccacg
     1981 gtgatgaaat agaacgccgc cgggaaaccg tcgaaaaccg cctgggcgat ctgcgccacc
     2041 atcgtcttct gctcgtaccc ggcgggcgcg ccgaccagct ggccggccgg atcctcggcc
     2101 atcttcacgc cggcgaactg ggcgagcagg atcaggccca tgagcatggt caccgagatg
     2161 ctcccgagta gcagcagcgt ggtggcggcg ttgcgggact tcggtttttg gaacgccgga
     2221 accccgttgc tgatcgcctc cacgccggtc agcgcggcac agccggacga gaacgcccgc
     2281 aggacgagaa acatcagagc aaaaccaacg agctggtcgc cctcaacgtg cagcccaaac
     2341 tcggcggact cggcgcgaag ttgctcatcg aacagcagca cccggataaa cccccaggcg
     2401 agcatggcga agatgccgat catgaatgcg taggtgggga tgg
//
LOCUS       DATADN010001068         1779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1326294, whole genome shotgun sequence.
ACCESSION   DATADN010001068 DATADN010000000
VERSION     DATADN010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326294"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VHH53_13720"
     CDS             complement(<1..637)
                     /locus_tag="VHH53_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2291218.1"
                     /translation="MDSYLQYLSENSIELAFSIQQHFLLVLYSVAFAAVISLALALVL
                     HTNLLTPPSWTRSLRVGSREAALLLASAALTIPSLALFGLLQPLLGLGVTPSIVALTL
                     YAIYPVLRNTVAGLSSVDPAVLEAARGVGMGPVRRMLRVQLPLAWPVIISGIRVAVLI
                     TISIAVVAALINGPGLGKPLQSGLARLGAVNSFNEVITGTLGCLVVAAGFEV"
     gene            868..>1779
                     /locus_tag="VHH53_13725"
     CDS             868..>1779
                     /locus_tag="VHH53_13725"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX2291219.1"
                     /translation="MDSELEQSRWWRIAAQIIEAGHTVSGDDLPRVINEAMGLVGMTA
                     QMYLVDLAQRTLRPVPKDHRAAVTVEGSLAGRAFQFVEAFAGTDPDEGVVLWLPMVDG
                     TDRLGVLRLGLPDGADGDDSRLRQRCSVLAGLIGHLVAAKLPYGDVLNVTRRSTPLTV
                     AAELLWQLVPPLTFTTRDLVITAVLEPHDQVGGDAFDYAVDGEVAFCGLFDAVGHDLQ
                     AGLATAVALAAIRNARRSGECNLSVIARRADAEIIAHGPHGRFVTAILARLHTGTGKL
                     SYVVAGHPPPLSLRANKTVKALQDGRRT"
BASE COUNT          317 a          578 c          602 g          282 t
ORIGIN      
        1 tgacttcgaa gccggctgcc accacgaggc atcccagcgt gccggtgatg acctcgttga
       61 acgagttcac ggcaccgagc cgggccaggc cgctctgcag cggcttgccc aagccgggac
      121 cgttgatcag ggccgccacc acggcgatcg agatcgtgat cagcaccgcg acccggatgc
      181 cggagatgat caccggccag gccaacggca gctgcacccg gagcatgcgg cgcaccgggc
      241 ccatcccgac gccacgcgca gcctccagca ctgcagggtc caccgaggac aggccggcca
      301 cggtgttgcg tagcaccggg tagatggcgt acagggtcag cgccacgatg ctcggggtga
      361 ccccgagccc cagcagcggc tgcaacaggc cgaacagcgc cagcgaagga atggtcaggg
      421 ctgccgaggc gagcagcagc gcagcctcgc ggctacccac ccgcaggctt cgggtccacg
      481 acggcggagt gagcaggttg gtgtgcagca ccagggccag tgccagtgag atcaccgcgg
      541 caaaggccac cgagtagagc accagcagga agtgctgctg aatggagaag gccagttcga
      601 tcgagttttc cgacaggtac tgcaggtagg agtccattag gtggctgcct catccgcacc
      661 gaccggctcg acgctggagg cggatctggg cctgcaggct cggccaatca tcacacctcc
      721 acgtgcaggt aatcgctatg gcgccgccag caccatgaag cgaggctagc gcggcatcac
      781 ccgatgtgcc ctggcgcggc tggcgtgcag ggggcgtcaa cgcagccccg cgttgggccg
      841 gcaccgcgaa cgctgttgga ttgaccagtg gattcggagc tggagcagtc gcggtggtgg
      901 cggatcgcgg cacagatcat cgaagcgggc cacacggtct cgggtgatga tctaccgcga
      961 gtgatcaatg aggcgatggg tctggtcggg atgacggccc agatgtatct ggtcgacctt
     1021 gctcaacgga cactgcggcc ggttccgaag gatcacaggg cagcggtcac ggtggaaggc
     1081 agcctggcgg ggcgcgcatt ccagttcgtc gaggccttcg ccggtaccga tcccgacgag
     1141 ggtgtggtgc tgtggcttcc gatggtcgat ggaaccgacc ggctcggcgt gctgcgcttg
     1201 ggattgccgg acggagccga cggtgacgac tccagattac ggcaacggtg ctcggtgcta
     1261 gccgggctga tcggccatct cgtggcggcc aagttgccct acggcgacgt gctgaacgtc
     1321 acccgccgga gcacgccact gaccgtggcc gctgagttgc tctggcagct cgtgccacca
     1381 ctcaccttca ccacccgcga cctggtcatc accgcggtgc tcgaaccgca cgaccaggtt
     1441 ggaggtgacg ctttcgacta cgcagtcgat ggtgaagtgg ccttctgcgg gctcttcgac
     1501 gccgttggcc acgacctcca agccggtctg gctaccgccg ttgccctcgc tgcgatacgc
     1561 aacgcccgcc gcagcggtga gtgcaatctc agtgtgattg cgcgtcgcgc cgacgccgag
     1621 atcatcgccc acggtccgca cggccgtttt gtcactgcga tcctggcgcg cctgcacacc
     1681 ggcaccggta agttgagcta tgtcgttgcc ggacatcccc cgccattgtc gctgcgcgcg
     1741 aacaagactg tgaaagcact tcaggatggc cgtcgaacc
//
LOCUS       DATADN010001069         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1494945, whole genome shotgun sequence.
ACCESSION   DATADN010001069 DATADN010000000
VERSION     DATADN010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494945"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VHH53_13730"
     CDS             <1..699
                     /locus_tag="VHH53_13730"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800272.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX2291220.1"
                     /translation="TTAVEKGLTETRPVRKRLPKKRPSETVSFTVGGAEGYLTAGSYP
                     DDGLGEIFVKMSKQGSTLAGVMDAFSMAISIALQYGVPLETYVSKFVNMRFEPAGMTD
                     DPDVRMASSVVDYLFRRIALDYLPKDKRDQLGILTAQERTEQVFAGYGSGAGADGGVD
                     VESLRTSVDSTAPIPAVQARPAPVYVGSSTELLELQMGKAADAPLCMTCGTKMRPAGS
                     CYVCEGCGSTSGCS"
     gene            866..>1635
                     /locus_tag="VHH53_13735"
     CDS             866..>1635
                     /locus_tag="VHH53_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2291221.1"
                     /translation="MPERALVLGGGGITGIAWELGMLAGLAEHGVDLRDADLVVGTSA
                     GSVVGALVTSGDDLEELYASQLLPPSLEPPIRMGIGTMVRWGWAVACSRGPQQVGLRV
                     GRMALTTATVPEAERREVIASRLQARTWPAQTLLVTAVDAESGEFTAFGQDSKVGRTA
                     VSLVDAVAASCSVPGVWPPTTIGGRRWIDGGVRSATNADLAAGYERVVVLAPLTRGFR
                     PAIGAAAHVAALRKQARVALVTPDANARRVIGRNVLDLA"
BASE COUNT          260 a          488 c          586 g          301 t
ORIGIN      
        1 accaccgctg tcgagaaggg attaaccgag accaggccgg ttcgtaagcg gctgcccaag
       61 aagcggccca gcgagaccgt ttccttcacc gtcggtggcg ccgagggcta cctcacggcg
      121 gggtcttacc cggatgacgg cctcggcgag atctttgtga agatgtccaa gcagggctcc
      181 acgttggccg gtgtgatgga cgccttctca atggcgatct caatagcgct gcagtacggg
      241 gtgccgctgg agacatacgt ctcgaaattc gtgaacatgc gtttcgagcc ggcggggatg
      301 accgatgacc cagacgttcg gatggccagc agcgtcgtgg actatctgtt ccgtcggatc
      361 gcgctggact acctgcctaa ggacaagcgc gaccaactgg gcatcctgac cgcacaggag
      421 cgcactgagc aggtgttcgc cggctacggc agcggcgcgg gggcggacgg cggtgtcgac
      481 gtggagtcgc tacgcacgtc ggtggactcc accgccccga tcccggcggt gcaggcccgc
      541 ccggcgccgg tgtacgtcgg tagctccacc gagttgctgg aacttcagat gggcaaggcc
      601 gcggacgcac cgctgtgcat gacctgcggg accaagatgc ggcctgctgg atcttgctat
      661 gtctgcgaag gctgcggttc caccagcggc tgcagctgaa gatctacgta tccggtcgct
      721 ccttgagaca gcggctcaga tctggggcgt tttgaaccgg tcacgtgcga ggtagcgaat
      781 attgacatga gccgcttcga catggttgcg ccatcgccgt cgcgcatact gatctcggct
      841 tgctgcgttg cgggaggagg ctaggatgcc ggagcgcgcg ctggtgctcg gcggcggtgg
      901 gatcaccggc attgcgtggg agttagggat gctcgccggg ctggctgagc atggggtgga
      961 tctgcgcgac gccgacctgg tggttggcac gtcagctggg tcggtggtgg gtgcactggt
     1021 cacctcaggt gacgatctcg aggagctcta cgccagtcag ctccttccgc cttcccttga
     1081 gccaccgatc cgaatgggga tcgggacgat ggtgcggtgg ggttgggccg ttgcctgttc
     1141 tcgaggcccg caacaggttg gactacgagt cggcaggatg gcgctgacga ctgcgacggt
     1201 tccggaggcc gagcgccgtg aagttatcgc ttcccggctg caagcacgta cctggccggc
     1261 ccagaccctg ctggtcacgg ccgtcgatgc cgaatcgggc gagttcaccg cctttggtca
     1321 ggacagcaag gtggggcgca ccgcagtgag cctggtcgat gccgttgcag ccagctgctc
     1381 cgtgccgggg gtgtggccgc cgaccaccat cggtgggcga cgctggatcg atggcggtgt
     1441 gcgctcggcg accaatgccg atctggcggc aggatacgag cgcgtcgttg tgctcgcccc
     1501 actcacgcgt ggtttccggc cggcgattgg tgcggctgcg catgtagccg cgttgcgcaa
     1561 gcaagcacgg gtcgcgctcg tcacaccgga cgccaacgcg cgacgtgtga tcgggcgcaa
     1621 cgtgctggac ctggc
//
LOCUS       DATADN010001070         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_734838, whole genome shotgun sequence.
ACCESSION   DATADN010001070 DATADN010000000
VERSION     DATADN010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734838"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..540)
                     /locus_tag="VHH53_13740"
     CDS             complement(103..540)
                     /locus_tag="VHH53_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291222.1"
                     /translation="MEVSAFRNIGSALAGITAGATAGGAIGSGLAGGGSFSYTPDQMR
                     SIVTRLTDLAHSYEKSLYDARHMALVEGPGLDHASRYHANAAVDSGKASLRSIEHEYQ
                     SCLALAQTFQDTLDAYLGTEERNVININQAGSQDDRTGPQPGI"
     gene            complement(629..1381)
                     /locus_tag="VHH53_13745"
     CDS             complement(629..1381)
                     /locus_tag="VHH53_13745"
                     /inference="COORDINATES: protein motif:HMM:NF025377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX secretion-associated protein EspG"
                     /protein_id="HEX2291223.1"
                     /translation="MHDTRTVELPVFVLINIVRRENLGEPPTVLAGGEQYVPARFEAQ
                     ARRILDAELARAGVRSDSHFRPLLELITKAQVEYYGWIDSQDGSLAVLAAARGRAAVL
                     VVRRGDTVVLGPTDANRLPQTVAAQLSDVPAARGPSLSVRAVDFSPARPAGGVLRQAT
                     AARPHQARLLDELLRQPRLGGARLYAATRDHTGTRQRSADSLTVLDVAQQGRWLVYFT
                     AKTGDRAINVVPGPPATVAAKLADLLPRPGRR"
BASE COUNT          262 a          508 c          555 g          255 t
ORIGIN      
        1 ttgttggttg cggaagtggt tgcccttgac tcgctgtggt gcagccggtc acgacgcaag
       61 tcgccgcgca tatcagggtc agaattgttc gaattctcat catcagatcc ccggttgcgg
      121 tccggtccgg tcgtcctggg accccgcttg gtttatgttg atgacgttac gttcctcggt
      181 gcccagatag gcgtcgagag tgtcctgaaa tgtctgcgcg agcgccagac aggactggta
      241 ctcgtgctcg atcgaccgaa gggaggcctt gccggagtca acggcggcat tggcgtggta
      301 ccggctcgcg tggtccaggc ctggtccttc gaccagcgcc atgtgacgcg cgtcgtatag
      361 agatttttcg taactatgcg caagatcagt tagtcgggtg acgatgctgc gcatctggtc
      421 gggtgtgtag gagaagctgc cgccgccagc caagccgctg ccgatggcgc cgccggcggt
      481 ggcgccagcg gtgatgccgg ccagggcgga gccgatgttt cggaatgcac tcacttccac
      541 gaggtcaacc ccctcgaaaa cgcgacccgg tcagacgata gcgcagaacg acacgtctga
      601 ctgcgctgag tgaccagcgg ttccccggtc accggcgacc gggcctgggc agcaggtcgg
      661 ccagcttggc ggccaccgtg gccggcggtc cgggcacgac gttgatggcg cggtcgccgg
      721 tcttggcggt gaagtacacg agccagcggc cctgctgggc gacgtcgagg acggtgagtg
      781 agtcggcgga tcgctggcgg gtgccggtgt gatcgcgggt cgcggcgtac agccgggcgc
      841 cgccgagccg gggctgtcgc agcagctcgt ccagcaggcg ggcctggtgc gggcgggcgg
      901 cggtggcctg acgcagcaca ccaccggccg gcctggccgg gctgaagtca acggcgcgga
      961 ccgacagcga cggcccacgg gcggcgggca cgtcggacag ttgggcggcc accgtctggg
     1021 gcaaccggtt cgcgtcggtc gggcccagca cgacggtgtc gccccggcga accacgagca
     1081 ccgccgcccg gccccgggca gcggccagca cggcgagcga tccatcctgg gaatcgatcc
     1141 aaccgtagta ctcgacctga gccttggtga tcaattccag gagcggccgg aaatgggagt
     1201 ccgatcggac cccggcccga gcaagctcgg catccaggat ccgccgcgcc tgcgcctcga
     1261 atcgagccgg aacgtactgc tcgccgccgg ccagaacggt aggcggctcg ccgaggtttt
     1321 cccggcgaac gatattgatc aacacgaaca ccggcagctc gacggtgcgc gtgtcgtgca
     1381 cgtgaccctc cccgaagccc ggccgaaagg gagcatacag cgaccgtcga gttctgccac
     1441 tgttgcacca gaacatcgcg aaccgactcg gccggtctcc gtgactgcaa ctcgaattgt
     1501 cggtctgaat ggccgcaatg cgggcccaag atctcagcga cctcgcccag gatcatcacg
     1561 ccggcggagc tgccgccggc
//
LOCUS       DATADN010001071         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1044872, whole genome shotgun sequence.
ACCESSION   DATADN010001071 DATADN010000000
VERSION     DATADN010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044872"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..955)
                     /locus_tag="VHH53_13750"
     CDS             complement(71..955)
                     /locus_tag="VHH53_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2291224.1"
                     /translation="MTLTMDEQAVQSYRELRGRLAEAEISERVDALTEMARTVRRLDL
                     GMIERAGLGHIGGDFSVTDILVTLYGAVLSVDPQNPQDPERDRFILSKGHSAGALYAT
                     LSVCGFFPEEELATFMAPLSALNGHPNRNKIPGVETNTGPLGHGFPVAVGHALAAKLQ
                     GSPRRTFVVLGDGELQEGSNWEAAMTAAHYDLSALTAIVDRNRLQQGARTEETKRLEP
                     LADKWRSFGFEVREIDGHDFAQLLDAIGTRGTDRPVCVIANTTKGKGVSFMEDRAEWH
                     HKVPSAEQVQIAREELSR"
     gene            1657..>1895
                     /locus_tag="VHH53_13755"
     CDS             1657..>1895
                     /locus_tag="VHH53_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291225.1"
                     /translation="MAVGELVVVVNQARGVRGDATASALLTPLLRSHVHRWSATPADS
                     SVRGRRPGPRPAEDPFSTCDDDERLRAAFTDPVDVR"
BASE COUNT          296 a          634 c          641 g          324 t
ORIGIN      
        1 gtcagcggct ggtcctcacc ggactccagc ccgatctcga catccggcag gaccgcgtcg
       61 gtaggacggt tcatcgactc agctcctcac gtgcgatctg gacctgctcg gcactgggca
      121 ccttgtggtg ccattccgcg cggtcctcca tgaaggacac gcccttgccc ttggtggtgt
      181 tggcgatcac gcagacgggc cgatccgtgc cccgcgtgcc gatcgcgtcg agcagctgtg
      241 cgaagtcgtg gccgtcgatc tcgcggacct cgaacccaaa gctgcgccac ttgtcggcaa
      301 gcggctcgag ccgcttggtt tcttccgtcc gcgccccttg ctgcagccgg ttgcggtcga
      361 cgatcgccgt cagggctgag aggtcgtagt gggcggcagt catcgccgcc tcccaattgc
      421 tgccctcctg caactcgccg tcaccgagga cgacgaaggt gcgccgcggc gacccttgga
      481 gtttcgctgc caacgcgtgt ccgactgcta cgggaaagcc gtgcccgagg ggaccggtgt
      541 tggtctcgac tcccggtatc ttgttgcggt tcggatgacc gttcagcgct gaaagcggcg
      601 ccatgaaggt cgcgagctcc tcctcaggga agaagccgca caccgacagg gtcgcgtaca
      661 gggctccggc gctgtgcccc ttgctgagga tgaaccgatc acgctcggga tcctgaggat
      721 tctgcggatc aacggacagg acggctccgt agagggtcac caggatgtca gtgaccgaga
      781 agtcaccccc gatgtggccg agcccggccc gctcgatcat cccgaggtcc agccggcgaa
      841 cggttcgcgc catctccgtc agcgcgtcga ccctttccga gatctccgcc tcggccagcc
      901 ggccccgcag ttcccggtac gactgaacag cctgctcgtc catggtgagc gtcatcgcag
      961 atccattcgt ggtgcgtgtg caagcaatgg ggtggtcacg ggacaggccc gtcgattcac
     1021 gacgtagcgg tgatgccgca cgaacacacg tggtcgttca cgtgcgatca cctccgtctg
     1081 caacgccgga gttcctccga ggaccaatgg ctgaggcacc ctccgctgcg gaggtccacc
     1141 gagcgggcgg gatgcgccgg gtcgaccgcg accaggcctc gtcgatcact gtcacctcca
     1201 ggccatcggt ggccgttgtg atcggcctca cagggccact gtggctgggg cgcaatgacc
     1261 acgaaagcga tctgtccgga atcggtcaca ccggtacggg tggcggccgg cgagcagagc
     1321 agctcacggt cgtcacggcc agcgacggcc tgacagtgcg ctgccgccgg gccggaggag
     1381 ccgcagcagc agtgccggag cgccactggt cttgcccgtt gtcggtgggc acttgcgcct
     1441 gtggccctgt tgaggctcac gccgtccggg ccctgaaggt ggaaccgtcg acgcctcaag
     1501 gccctccttg atgactgtcc aacgctgggt tcctggacag gcagcaggcg tggctacgcc
     1561 ggtgtccgcg gggccaggtg cgatgcccgc gggaaagggc atgagctgac cgggcttcaa
     1621 ccggcgctcg acacggtgga ggaaggaggt gccgcgatgg ccgtcggcga gctggttgtt
     1681 gtcgtcaacc aggcccgggg ggtgagaggc gacgcgaccg cgtcagcgct gctgacgccg
     1741 ctactgcgga gccacgtgca ccgatggtcc gcgacaccag cggattcgtc cgtccgtgga
     1801 cgacggcctg gacctcgacc ggccgaggat ccgttttcaa cttgcgacga cgacgagcgc
     1861 ctgcgggccg ccttcaccga ccccgtcgac gttcg
//
LOCUS       DATADN010001072         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1195646, whole genome shotgun sequence.
ACCESSION   DATADN010001072 DATADN010000000
VERSION     DATADN010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195646"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VHH53_13760"
     CDS             complement(<1..445)
                     /locus_tag="VHH53_13760"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2291226.1"
                     /translation="MVLIDQTITYLEMTSPDQLLAGRAPPAEVTINSVDATALALIRS
                     THDRIATPHHWSSLDWSEQQWSDLLARRGVRSWTAHIGVEAIGLVQLEIHPGHDVEII
                     KFGLVPEFVGRKFGGHLLTLATRLAWGVGGAHRVWLHTSSFDHPHA"
     gene            complement(592..921)
                     /locus_tag="VHH53_13765"
     CDS             complement(592..921)
                     /locus_tag="VHH53_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291227.1"
                     /translation="MGIGKAADQLVRRDNNKEVNHRGGDQKSDHRTDERPVAECLTED
                     DEPSSHVPASGENGDQRSEERLVELHHQVSERGADNHRDSKIDDVAAENEFTKAFEHR
                     GTPDYLM"
     gene            complement(899..>1688)
                     /gene="rsgA"
                     /locus_tag="VHH53_13770"
     CDS             complement(899..>1688)
                     /gene="rsgA"
                     /locus_tag="VHH53_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX2291228.1"
                     /translation="ERSSVLRRTADDTDPYERIVVANAEQLLIVASLADPPPRTGLID
                     RCLVAGYAGGLKPVLCLTKRDLAAPDAVLERYAALDLPVLVTAPQTGVDAVRSRLADQ
                     VSVLVGHSGVGKSTLVNRLVPDAFRVTAAVSGVGKGRHTSSSAVAMPLPGGGWVVDTP
                     GIRSFGLAHVTPDDVVRAFADLAGAIANCPRGCTHLGPPADPECALDGLDGAAHRRAM
                     SVRGLLAALAAESRSPTRQYRAPAFWPCAPRAVPRSPGGHRKSG"
BASE COUNT          293 a          546 c          562 g          287 t
ORIGIN      
        1 gtgcatgcgg atgatcgaac gaggaggtgt gcagccacac gcggtgggct ccacccacgc
       61 cccaggcgag ccgggtggcc agggtgagta aatggccgcc gaatttccgg ccgacgaact
      121 ctggaaccaa tccgaacttg atgatttcga cgtcgtggcc gggatggatc tccagctgca
      181 ccagcccgat ggcttctaca ccgatgtgcg cggtccagga tcgcacgccg cgccgagcca
      241 gaagatccga ccactgttgt tccgaccagt ccaggctcga ccaatgatgt ggtgtggcga
      301 tccggtcgtg agttgagcga atcagcgcca gtgcggtcgc gtccacgctg ttgatcgtga
      361 cctcagcggg cggcgcccgg ccggcgagaa gctggtcggg agacgtcatc tccaggtagg
      421 tgatggtctg atcgatgagc accatctcac gatgccacaa gtgacgtcgc cacaatgacc
      481 tcgcccttac cacgaagctc accggagcaa agctacggct agatttcact acaccgggta
      541 ctgaagtagt gggccggcgt tgtgacggtg gccacgtgga atgagatgcc gtcacatcaa
      601 gtaatcgggg gttcccctgt gttcaaaggc tttcgtgaat tcattctccg cggcaacatc
      661 gtcgatcttg ctgtcgcggt ggttatcggc accgcgttcg ctgacttggt gatgcagttc
      721 aaccaatcgt tcctcactcc tctggtcgcc gttttcaccg gaggcgggga cgtgggagga
      781 aggttcgtcg tcctcggtca gacattcggc tacggggcgt tcgtcggtgc ggtgatcact
      841 tttttgatca ccgccgcggt ggtttacctc tttgttgttg tcccgacgaa caagctgatc
      901 agccgctttt ccgatgccca cccggagaac gaggcacagc cctcggcgcg caaggccaaa
      961 atgccggcgc gcgatactga cgcgtcggcg agcgagactc agcggcgagt gcggcgagca
     1021 gcccccggac ggacatggcc cggcggtggg cggcaccgtc gaggccatcc agcgcgcact
     1081 ccggatcggc gggtgggccg agatgggtgc aaccccgtgg acagttcgcg atcgcgcctg
     1141 ccaggtcggc gaaggcgcgg accacatcgt cgggcgtcac gtgcgccaac ccgaaggaac
     1201 ggatccccgg cgtgtccacc acccacccgc cgccgggtag cggcattgcc accgcggagc
     1261 tggaggtgtg ccggcccttg ccaaccccgc tgaccgccgc ggtgacccgg aaagcatccg
     1321 gcaccagccg gttcaccagg gtcgacttgc cgactccgga gtgcccgacc aggaccgaga
     1381 cctgatcagc cagccggctg cgcaccgcgt ccacccccgt ctgcggggcg gtgacgagca
     1441 ctggcaggtc cagcgcggcg tagcgctcca gcacggcgtc cggtgcggcg agatcccgct
     1501 tggtcaggca gagcaccggc ttcaggcctc cggcgtagcc ggccaccagg caccggtcga
     1561 tcaacccggt acgcggcggt ggatcggcca gcgacgccac gatcagcagc tgctcggcgt
     1621 tggcgaccac gatgcgctcg taggggtcag tgtcgtcggc ggtgcgacgc agcacgctgg
     1681 accgctcg
//
LOCUS       DATADN010001073         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_442378, whole genome shotgun sequence.
ACCESSION   DATADN010001073 DATADN010000000
VERSION     DATADN010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442378"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VHH53_13775"
     CDS             complement(<1..842)
                     /locus_tag="VHH53_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291229.1"
                     /translation="MPVDLVAVQADDALLDLLGSVDRTPRDVDQMPGGVDGELVRVLR
                     AWRHEVHADSIRELVDTDTALAAIGAARRPARRRNLVFAPVAAAAAVFVIAFSAVGLL
                     AKSAHPGDNLWGVTQVLYSDYARSVEAAAFVQAELQEAKTALQQGKPEQAKASLDRIQ
                     QQLPAIAEAEGRTDLTARHHELARELGGNPSGQVAPPGSPNAGSSEQREASLAPTPAQ
                     PNPAPEQYPGPEQYPGPEQYPAPDQYPAPEPNPGPEPNPGPKPNPGPKPNPAPPPPIP
                     NPPSP"
     gene            complement(901..1473)
                     /locus_tag="VHH53_13780"
     CDS             complement(901..1473)
                     /locus_tag="VHH53_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2291230.1"
                     /translation="MTTVGDDLDVLVGAAVDGDRDAVDGVLRWIRPLVLRYCRARVGS
                     QEKTFASADDVAQEVCLAVFAALPSYRDQGRPFLAFVYGIAAHKVADAHRSAARNRAE
                     PVPEVPDSPALGDGPEQRAMQGELAGRMDKLLELLPDKQREILVLRVVVGLSAEETAE
                     AVGSTPGAVRVAQHRALGRLRNVLTSEGVV"
     gene            complement(1700..2296)
                     /locus_tag="VHH53_13785"
     CDS             complement(1700..2296)
                     /locus_tag="VHH53_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2291231.1"
                     /translation="MTAVLICDDRRSVREGLTRVMSAVPGVARIDCVAHGDELLARYS
                     RQPVDVVLVGTQRAVPTGVEATRRLVSAHPQANVIVFGAPDDAGSIAAAIAGGARGYL
                     RWDASRPELVAALAHTLASTSVPAPRPPSDPGVKLTERELQVLRGMSQGKSNGQIGRE
                     LYLSEDTVKTHARRLFRKLGVRDRAQAVAHGFRRGLVA"
BASE COUNT          466 a          889 c          870 g          419 t
ORIGIN      
        1 ggcgaaggcg ggtttgggat aggcgggggc ggagcagggt ttggcttcgg gccagggttc
       61 ggcttcgggc cagggttcgg ctccgggcca gggttcggct ccggggcagg gtattgatcc
      121 ggggcagggt attgctccgg acctgggtat tgctccggac ctgggtattg ctccggggcc
      181 gggtttggct gtgcgggcgt gggggccaac gacgcctcac gttgttccga cgatccggcg
      241 tttggcgatc caggcggcgc aacttgcccg gacggattcc cacccagctc ccgcgcgagt
      301 tcatgatggc gagcggtgag atcggtgcgg ccctcggcct cggcgatcgc cggaagctgc
      361 tgttgaatgc ggtccagcga cgccttggcc tgctccggct tgccttgctg caacgccgtc
      421 ttggcctctt ggagttcggc ctggacgaag gccgcggcct caaccgaacg ggcgtagtcg
      481 gaatagagca cctgagtcac gccccacaag ttgtcgccag gatgggcgga cttggcgagc
      541 aggccgactg cggagaaggc aatcacgaac accgcagcgg ccgccgcgac cggcgcgaag
      601 acgagattac gtcggcgggc cggccggcgg gcggcgccga tcgccgccaa ggcggtatcg
      661 gtgtccacca gctctctgat cgagtcggca tgcacctcgt ggcgccacgc cctcaggaca
      721 cgcaccagct cgccgtcgac accgcccggc atctgatcga catccctcgg cgtgcgatcg
      781 acactgccga gcagatccag cagcgcgtcg tcggcctgga cggcgaccag atcaacaggc
      841 atctcagagg gatctccatc ggggtggcta acacgtgggt cgccgttatg accggacatc
      901 tcagaccacc ccctcggacg tcagcacatt gcgtaaccgg cccagcgccc ggtgctgggc
      961 tacccggacc gcaccaggag tggagcccac cgcctcggcg gtctcctctg ctgacagccc
     1021 taccacaacc ctcagcacga gaatctcgcg ctgtttgtcc ggcaggagct ccagcagctt
     1081 gtccatccgt ccggccaact cgccctgcat cgcacgctgc tccggaccgt cacccaacgc
     1141 cggggaatcg gggacctccg ggactggctc ggcacgattg cgggccgccg aacggtgggc
     1201 gtcagccacc ttgtgcgccg cgatgccgta cacgaaggca agaaatggcc gaccttgatc
     1261 gcggtaactg ggcagagccg caaacaccgc gagacacacc tcctgggcta catcatccgc
     1321 cgaggcgaac gttttctcct ggctaccgac gcgcgcgcgg cagtaccgca acacgagagg
     1381 acggatccag cggagcacac catcgacggc atctcggtca ccgtcgacag cagcaccaac
     1441 cagaacgtcg aggtcgtccc ccacagtggt catcgcagaa cgcagccctg ccgttacgac
     1501 gtggaccagc cggactgcat gccagctggc ggtggacatt ccgctaccca ggacatgatc
     1561 atcagcccaa gaccacggca cctcaggcgc aaccgtagta ggcgacagat caaatgccga
     1621 acgcggtgtc tctgcgcata cgcgcgttca cagcgccgtg ggtttcgatg caaatccccg
     1681 gctcgcggtg cagggcggct caggcgacca gaccgcgccg gaaaccgtgt gccacggcct
     1741 gagcccgatc ccgcacgccg agcttgcgga acagtctccg ggcgtgagtt ttcacggtgt
     1801 cctcggagag atagagctca cgcccgatct ggccgttgct cttgccttgg ctcatcccgc
     1861 gcagcacttg cagctcgcgc tcggtgagct taactccggg atcggacggg ggtcgcggcg
     1921 ccggcaccga agtactggcc agcgtgtgcg ccagcgccgc cacgagctcc ggtcgcgagg
     1981 catcccaacg cagataacct cgggcaccgc cagcgatcgc ggcggcgata ctgccggcgt
     2041 cgtccggcgc accaaacacg atgacgttgg cctgagggtg cgccgacacc agccggcgag
     2101 tggcctccac accggttggc accgcacgct gggtgcccac caggacgaca tccacaggct
     2161 gccgggagta gcgagctagc agctcatcgc cgtgtgccac gcagtcgatc cgagcaacgc
     2221 cggggaccgc ggacatcacg cgagtgagtc cctcacggac gcttcgccgg tcgtcgcaga
     2281 tgagaactgc ggtcacggga gggctccttt cctgcagcca agtgatatgc ccacctccct
     2341 atcggtcgta cggggccgga ccttgacacg atctgatgga aattcgccaa gaaccggttc
     2401 cgcactactc cgaacggctg gtgtccgcgt ggagcaacgc tcactctacc gagtgtgagc
     2461 ggttcagggc agtaccgcga atacggatga ttcggacatc tggctacgta gtgacgcagt
     2521 gacggccctc gtccctcact gatgaggggc aagggccgtt actgcttgcg ggtggcgtca
     2581 cacggtggac ggtgacttac atacctggcc cgcagccggt cacggtcatg ggcacggcca
     2641 cggg
//
LOCUS       DATADN010001074         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1495319, whole genome shotgun sequence.
ACCESSION   DATADN010001074 DATADN010000000
VERSION     DATADN010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495319"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VHH53_13790"
     CDS             complement(<1..851)
                     /locus_tag="VHH53_13790"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2291232.1"
                     /translation="MADRGNAMPSFDYDLAIIGAGSGGLTAARLARFFAKRVALIDKD
                     RLGGDCLHYGCVPSKALIKASRVAHEAATAQRWGLSAQPLNNGLASVNARVQRAIVDI
                     GKLDDTAALSELGVDVLLGGATFRDPHTLRVADRDITAKIILIASGSSPAVPAIEGLA
                     EVGYLTNEDAFDLSDIPGRLAVIGGGPIGVELAQAFHRLGSTVTVLQRGPHLIPRDDT
                     EIIDVLERQFAEEGINVRLDTRLHAVRRDGVDAVLTLDDGQLRVDKVLCAVGRIPNTE
                     SLGLDAAG"
     gene            complement(864..1265)
                     /locus_tag="VHH53_13795"
     CDS             complement(864..1265)
                     /locus_tag="VHH53_13795"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
                     /protein_id="HEX2291233.1"
                     /translation="MQVREQAAGYPIGLVACGFSSLDALAALADHVGWPGLFLADENR
                     RLYELLGMRRARLWQVYSPGTLARYALAAARGQQLPRPVEDIRQMGGDALLHDGVVVR
                     RWLPRTPNDRVSPARLVGGSSRATNHPKRCP"
     gene            complement(1287..1418)
                     /locus_tag="VHH53_13800"
     CDS             complement(1287..1418)
                     /locus_tag="VHH53_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291234.1"
                     /translation="MPREIDQPVPLDGLELPDARTGRIVHLGALPGRYLLTAIRHRF"
     gene            complement(1408..>1633)
                     /locus_tag="VHH53_13805"
     CDS             complement(1408..>1633)
                     /locus_tag="VHH53_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291235.1"
                     /translation="AADVTPAWELMQRGEKASDPRILATQERLASCHYAMAHPETGEL
                     VPACVQHSVLDPIENIELRRLLPIVDVRAT"
BASE COUNT          259 a          534 c          573 g          267 t
ORIGIN      
        1 ccggcggcgt cgagtccaag cgattccgta ttgggtattc gtccgaccgc gcacaggact
       61 ttgtccaccc gcagttggcc gtcgtcgagg gtgagcaccg catccacgcc gtcgcggcgc
      121 accgcgtgca gccgggtatc cagccgcacg ttgatgccct cctcggcgaa ctggcgctcc
      181 agcacgtcga tgatctcggt gtcatcgcgg ggaatcagat gcggcccgcg ctgcagcacg
      241 gtcaccgtgg agccgagtcg gtgaaatgcc tgcgcgagct ctacgccgat cggaccgcca
      301 ccaatcaccg caagccggcc cgggatgtcg ctgaggtcga aggcgtcttc gttggtgagg
      361 taaccgacct cggccagccc ctcgatggca ggtacggccg gactgctgcc cgaggcgatc
      421 aggatgatct tggcggtgat atctcggtct gccacgcgca gtgtgtgtgg gtcgcggaag
      481 gtggcgccgc cgagcaggac atccactccg agttcggaca gtgccgcggt gtcgtccagc
      541 ttgccgatgt cgacgatggc tcgctgcacc cgggcgttga ccgaggcgag cccgttgttc
      601 agcggctgcg cggacagtcc ccaccgctgt gcggtggcgg cctcgtgagc cacccgcgac
      661 gccttgatca acgccttgct cggcacgcag ccgtaatgca ggcagtcccc accgagccgg
      721 tctttgtcga tcaatgcgac ccgcttggcg aaaaaccggg cgagtcgggc ggcggtcagc
      781 ccgcccgatc ccgcgccgat gatcgctagg tcgtagtcga aggacggcat cgcgtttccc
      841 cgatcagcca cggttggtcg tcgttacggg cagcgcttgg ggtggttggt ggctcttgaa
      901 gagccaccaa ctaggcgtgc tgggctgacc cggtcgttgg gcgtgcgcgg cagccaccgg
      961 cgcacgacga cgccatcgtg cagcagcgcg tcgccgccca tctgccggat atcctctacc
     1021 ggccgtggta gctgctgccc gcgcgctgcg gcgagcgcgt agcgggccag ggttcccggc
     1081 gagtacacct gccacagcct ggcacggcgc atgccgagca attcgtagag ccggcggttc
     1141 tcatcggcga ggaacagacc cggccagccg acatggtcgg cgagggcggc gagcgcgtca
     1201 agtgaactga acccgcacgc gaccaagcca atcgggtatc cagcagcctg ctcgcgcacc
     1261 tgcacgaggt gctgttggca gagcagtcaa aagcggtgcc ggatcgcggt gagcaggtac
     1321 ctgccgggca gtgcgccgag gtgcacgatc cggccggtgc gcgcatcggg cagctcgagc
     1381 ccgtcgagcg ggacgggctg atcgatttca cgtggcacgg acgtcgacga tgggcagcaa
     1441 ccggcggagt tcgatgttct cgatcgggtc gagcaccgaa tgctgcacgc aggccggaac
     1501 gagttcaccg gtctcggggt gagccatcgc gtaatggcag gacgcgagcc gttcctgggt
     1561 ggcgaggatc cgcggatcgg aagctttctc gccgcgttgc atcagctccc acgccggcgt
     1621 gacgtccgcg gcg
//
LOCUS       DATADN010001075         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1195871, whole genome shotgun sequence.
ACCESSION   DATADN010001075 DATADN010000000
VERSION     DATADN010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195871"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VHH53_13810"
     CDS             complement(<1..408)
                     /locus_tag="VHH53_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX2291236.1"
                     /translation="MSLHLYDTATRSLREFVPLRPRTASIYVCGATVQGVPHIGHVRS
                     ALNYDVLRRWLMATGHDVLLVRNVTDIDDKILAKSAEAGRPWWEWAYIHERAFEDAYT
                     ALGCLPPSALPRATGHVPQMVELMARLIDRGHAY"
     gene            complement(389..874)
                     /gene="ispF"
                     /locus_tag="VHH53_13815"
     CDS             complement(389..874)
                     /gene="ispF"
                     /locus_tag="VHH53_13815"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX2291237.1"
                     /translation="MRVGIGTDVHRVQPGRECWLAGLHWPGITGCAGHSDGDVAAHAL
                     CDAALSAAGLGDLGAVFGTGEQRWAGAHGAELLTEVRRRLTAGGWTVSNGAVQVIGNA
                     PRVGTRRAEAQRVLSEALGGPISVTGTTTDGLGLTGRGEGIAAVATVLLVLSPGEPAP
                     V"
     gene            complement(871..1572)
                     /gene="ispD"
                     /locus_tag="VHH53_13820"
     CDS             complement(871..1572)
                     /gene="ispD"
                     /locus_tag="VHH53_13820"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX2291238.1"
                     /translation="MVAHAPARIVALVPAAGQGVRLAAGMPKAFVPLLGRPLLWHCVQ
                     GLLAAGCVDLVVVAVGAQYRDQAATALADAGRRVRIVTGGAQRSDSVRCALDSVPDAE
                     VVLVHDAARCLTPVGVIQAVVQAVVAGRRAVVPVVTVVDTIKQVDPEGRVVSTVDRTA
                     LRVVQTPQGFAADLLRRAHQRPSGPVTDDAGLVEQLGETVTILPGHPHAFKITTPLDL
                     VVAETVASAVAAGRV"
     gene            complement(1591..2079)
                     /locus_tag="VHH53_13825"
     CDS             complement(1591..2079)
                     /locus_tag="VHH53_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX2291239.1"
                     /translation="MVFKVGETVVYPHHGAALIEAIETRVIKGEEKKYLVLKVAQGDL
                     TVRVPAANAEVVGVRDVVGQEGLDRVFEVLRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRRERDRGLSAGEKRMLAKARQILVSELALAEGTNEDNAEVLLDEVLAA
                     AS"
BASE COUNT          403 a          863 c          743 g          367 t
ORIGIN      
        1 gtaggcgtgc ccccggtcga tcagccgtgc catcagctcg accatctgcg gaacatggcc
       61 ggtagcgcgg ggtagtgccg acggcggcag gcaacccaga gcggtgtaag catcctcgaa
      121 cgcccgttcg tggatgtacg cccactccca ccacggccgt cccgcctcgg cggacttggc
      181 aaggatcttg tcgtcgatat cggtgacgtt gcgcaccaga agcacgtcgt ggccggtggc
      241 catcagccag cgacgcagca cgtcgtagtt caacgctgaa cgcacgtgcc cgatgtgcgg
      301 gacgccctgc acggtggccc cacacacgta gatcgacgcc gtgcggggtc gcagcgggac
      361 gaattcccgc aggctgcgcg tcgcggtgtc atacaggtgc aggctcacca ggtgatagta
      421 cgagcagcac cgttgccacc gcggcgatgc cctcgccacg gccggtgagg ccgaggccgt
      481 cggtagtggt gccggtgacc gagatgggcc cacccaacgc ctcggacagc acccgttgcg
      541 cctcggcgcg gcgggtgcct acccgcggcg cgttcccgat cacctgcacc gcgccattgc
      601 tgaccgtcca gcccccggcg gtcagcctgc gacgtacttc ggtgagcagt tcggcgccgt
      661 gcgccccggc ccaacgttgc tcgccggtgc cgaataccgc acccagatcg cccaaaccag
      721 cggcggacag agcggcgtcg cacaacgcat gcgcggcgac gtcgccgtcg gagtgcccgg
      781 cacaacctgt tattccaggc cagtgcagtc cggcgagcca gcactcccgg ccgggttgta
      841 cccggtgcac gtcagtgccg atcccaaccc tcatacccga ccggctgcta ccgcgctggc
      901 caccgtctcg gcgaccacga ggtccaacgg ggtagtgatc ttaaaagcgt gcgggtgacc
      961 aggcagtatg gtcaccgttt cccccagctg ctcgaccaga ccggcatcgt cagtcaccgg
     1021 gccggacggc cgctggtgtg ctcggcgcag caggtccgcg gcgaatccct gcggtgtctg
     1081 aaccacccgc aaggccgtac ggtccactgt cgacaccacc cggccctcgg ggtctacctg
     1141 cttaatcgtg tcaaccacgg tgacaaccgg taccacggca cgccttccgg ccactaccgc
     1201 ctgcaccacc gcctggatca caccgaccgg ggtgaggcag cgggcggcgt catgcaccag
     1261 caccacctcg gcgtccggca cgctgtccag ggcacagcgc acggagtcgg accgttgcgc
     1321 cccgccggtc acaatccgca cccgccgccc cgcatcggcg agtgcggtgg ctgcctgatc
     1381 ccggtactga gcaccgaccg caaccacgac aaggtcgacg caaccggcag ccagcagccc
     1441 ttgcacgcag tgccagagca acggccgccc cagcagcggc acgaatgcct tgggcatccc
     1501 ggccgccaac cgcacgccct ggccagcagc tggtaccagc gcgacgatcc gagccggggc
     1561 gtgggctacc accccagccg ccggtcggcg tcaggaggcg gcggcgagga cctcgtcgag
     1621 gagcacctcg gcgttgtcct cgttggtgcc ctcggcgagc gcgagctcac tcaccagaat
     1681 ctggcgggcc ttcgcgagca tgcgtttctc gccggccgac agaccgcggt cacgctcgcg
     1741 acgccacaga tcccggacca cttccgccac tttgttgacg tcgccagagg cgagcttctc
     1801 caagttggcc ttgtaccgcc gcgaccagtt cgtcggctcc tcggtgtggg gagcacgcag
     1861 cacctcgaaa acccggtcga ggccctcctg gccgaccacg tcccggacgc cgacgacttc
     1921 ggcgttggca gcgggaacac ggacggtaag atcaccctga gcgaccttga ggacgaggta
     1981 cttcttctcc tcacccttga tcactcgagt ctcgatggcc tcgataagag cggcaccgtg
     2041 gtgcgggtag acgacggtct ctccgacctt gaaaaccatg tgtcctctgc ccctttcgct
     2101 acacctaggt taccacgcct ggaaatcgtc ctgcaccggg tatcacggcg tcatcgcagg
     2161 tcagaggatc ggcaaccgac tctcaccagc caacgctgcg ctcccgcagc tcaaggtgac
     2221 tccactgtag accgacacca gacctgcact agaccgacac ccgacccacc cggtgccggt
     2281 ctggcgccgg ctcatgtctg gtctggtgta ggtctacagt ggagtcacct tgagctgcgg
     2341 gagcgcggcg ttgcttggtg agacttggtt gccgat
//
LOCUS       DATADN010001076         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_273839, whole genome shotgun sequence.
ACCESSION   DATADN010001076 DATADN010000000
VERSION     DATADN010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273839"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="bioA"
                     /locus_tag="VHH53_13830"
     CDS             <1..998
                     /gene="bioA"
                     /locus_tag="VHH53_13830"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX2291240.1"
                     /translation="LATRLVEITPEPLRHVFLCDSGSVSVEGAIKMCLQYWRSRRRPG
                     KHRLLTWRGDYHGDTFGAMSVCDPDGGMHSLWSDVLAQQVFVPPPPAGLDLAYVQLLV
                     DTVERHADELAAVIVEPVVQGTGGMRFHDPRYLHVLREITLAHDVLLVFDEIATGFGR
                     TGMLFAADHAGISPDVMCVGKALTGGYLTMAATLCTPRVADGISGGEVAVLAHGPTFM
                     ANPLAAAVASASIELLLGQDWRAEVRRLEAGLRAGLGRARELPGVIDVRVLGAIGVVQ
                     LDHQVDIRAATAAAVAHGVWLRPFRDLIYTMPPYITGNDDLARITHGVLAAVTAG"
     gene            1017..>1972
                     /locus_tag="VHH53_13835"
     CDS             1017..>1972
                     /locus_tag="VHH53_13835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2291241.1"
                     /translation="MSAPEFPTTATAYQSMLSAVIERTVGPQAHEIDRTGAYPRDSVQ
                     ALGEAGLLGLISAPGVGGGGESLAAAAHVVEALATRCGSTAMVVLTHYAAVAVIEAHG
                     PQDIRRLIARGDHVSSLALPEVGSGSDFWAPVGTATAGDGDTVKLDARKSWVTSAGHA
                     DSYVWSSRPLDGDGAMTLWLVPSGAAGLRVGKFDGLGVRGTASGPVSAAGVVVPGDAR
                     LGDDGAGLDIALATALPCFLMLNAAFSLGVMEALIAEAAAHLARTRLQHLDQTLAQQP
                     TSRAAFARLRIRTDATRAFLRDTLIALDTRREDATLRMLEVKA"
BASE COUNT          273 a          668 c          710 g          321 t
ORIGIN      
        1 ggctggcgac ccggctcgtg gagatcaccc ccgagccgct gcggcacgtg ttcctgtgcg
       61 actccggctc ggtgtcagtc gagggcgcga tcaagatgtg cctgcagtac tggcgctcgc
      121 gtcggcggcc cggcaagcac cggttactca cctggcgagg cgattatcac ggtgacacct
      181 tcggcgcgat gagcgtctgt gatcccgacg gcgggatgca ctcgctgtgg tccgacgtgc
      241 tggcccagca ggtgttcgtg ccgccaccgc ccgccggact ggacctggca tacgtgcagc
      301 tactggtcga caccgtggag cgccacgccg acgagctggc cgcggtgatc gtcgaaccgg
      361 tggttcaggg cacgggaggc atgcgctttc atgacccgcg ctacttgcac gtgctgcggg
      421 agatcaccct ggcccacgac gtgctcctgg ttttcgacga gatcgccacc gggttcggcc
      481 gcaccgggat gctgttcgcc gccgaccatg ccgggatcag ccccgacgtg atgtgcgtgg
      541 gcaaggcgct caccggcggt tacctcacaa tggcggccac actgtgcacc ccgcgggtcg
      601 ccgacggcat ttcgggtggc gaggttgcgg tgcttgccca cgggccgacg ttcatggcca
      661 acccactggc ggcggcggtg gcgtcggcgt cgatcgagtt gctgctcggt caggactggc
      721 gcgccgaagt gcggcgcctg gaggcggggc tacgagcggg cttggggcgg gcgcgggaac
      781 taccaggggt gatcgacgtt cgggtgctgg gagcgatcgg cgtcgtgcag ctcgaccatc
      841 aggtggacat ccgcgctgcc accgccgccg cggtggccca cggcgtctgg ttgcgcccct
      901 tccgtgacct catttacacc atgccgccct acatcaccgg caacgacgac ctggcgcgga
      961 tcacccacgg ggtgctcgcc gcggtcaccg ccggctgacg tggcagcctg tgctccatga
     1021 gtgctccaga gtttccgacg accgcgacgg cctaccaatc gatgctgtcg gcggtcatcg
     1081 agcgcacggt cggcccgcag gcccacgaga tcgaccgcac cggagcgtat ccgcgcgaca
     1141 gcgtgcaggc tctgggcgag gccgggctgc tcggcctgat cagcgcccct ggagtaggcg
     1201 gtggtggcga gtctctggca gcggccgcgc acgtcgtcga ggcgctggcc accagatgcg
     1261 gctcgacggc catggtggtg ttgacgcact acgccgccgt cgctgtgatc gaagcgcacg
     1321 gaccgcagga catccgccgc ctgatcgccc gcggcgacca cgtgagcagc ctggccttgc
     1381 ccgaggtcgg ctccggcagt gacttctggg cgccggtggg caccgccacc gctggcgacg
     1441 gcgacaccgt aaaactcgat gctcgcaaga gctgggtgac ctcggccggt cacgccgaca
     1501 gctacgtatg gtcaagccgg ccactggatg gcgacggcgc gatgaccctg tggttggtgc
     1561 ccagtggcgc ggccgggttg cgcgtgggca agttcgacgg gctgggcgtg cgtggcaccg
     1621 cctccgggcc ggtctcggcg gcgggcgtgg tagtccctgg cgacgcccgg ctgggtgacg
     1681 acggggcggg gctggatatc gcgctggcca ccgcgctgcc ctgcttcctc atgctcaacg
     1741 cggcgttctc gctgggcgtc atggaggcct tgatcgccga ggccgcggcg caccttgccc
     1801 gcacccggct gcagcatctc gaccagacac tggcgcagca gccgacgtcc cgggcggcat
     1861 tcgcccggtt gcgtatccgc actgacgcca cccgggcatt cctgcgggac actttgattg
     1921 ccctagatac caggcgggag gatgcaacgc ttcgcatgct ggaggtcaag gc
//
LOCUS       DATADN010001077         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_117111, whole genome shotgun sequence.
ACCESSION   DATADN010001077 DATADN010000000
VERSION     DATADN010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117111"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1392
                     /locus_tag="VHH53_13840"
     CDS             1..1392
                     /locus_tag="VHH53_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2291242.1"
                     /translation="MFDPGTLAELAPRDASGTFAARGPIDGTTAIAYCTDATTMGGAM
                     GWQGCQHIVTAIDTAIQQRCPVIGLWHSGGARLAEGVEALDAVGQVFSAMVRASGRVP
                     QISVVLGAAAGGAAYGPALTDIVIMAPEGRVFVTGPDVVRSVTGEQIDMESLGGPSAH
                     GKKSGVVHVMADSESDALLRARHIAGLLARPGMFDLDSVREEVDLSEHLPAEQQRAYD
                     VRPLVRAVLDQPDPNPDAGADTSSDPAGGFVELQPKWAPNVVVGLGRLAGRTVGVIAN
                     NPLRLGGCLDSVSAEKASRFVRMCDSFGVPLLVLVDVPGYLPGVGQEWDGVVRRGAKL
                     LHAFAEAVFPRVTLVTRKAFGGAYIAMNSRSLGATQVFAWPDAQVAVMGAKAAVGILH
                     RKALAAAAQEEREALHAKLAEHHERIAGGVDRAVAIGVVDEVVKPAQTRRRLAEALAA
                     APESRGGHGNIPL"
     gene            complement(1397..1936)
                     /locus_tag="VHH53_13845"
     CDS             complement(1397..1936)
                     /locus_tag="VHH53_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291243.1"
                     /translation="MTVAVLAAAVGAGVLVRQLQPTASADGAASIAHNAPSHAPAPGP
                     GLTTVALVEDVAVHPDANRVRRVLQKYFDAINAGDYKLWRSTVTPQHARDTGEAAWRD
                     QYRSTLDGSIVLHRLEPRAGGGLVALLSFTSLQDPADAPPDMPVRCLRWWVSYPLIGD
                     AGELRLSPSSPSTSLRVPC"
     gene            complement(1997..2488)
                     /locus_tag="VHH53_13850"
     CDS             complement(1997..2488)
                     /locus_tag="VHH53_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HEX2291244.1"
                     /translation="MSTSGTIYVHSSPSAVCPHVEWAIAGALQTRADLQWTAQAAAPG
                     QLRAECDWSGQPGVAAAVVNALRAWPMLRFEVTEQPSPGVDGERFCFVPGLGLWRART
                     SANGDIVVGEDQLRALAANARSGETFAHLLDELLGAAWDEALEPFRRAADGAPVTWLH
                     RVG"
     gene            complement(2611..4002)
                     /locus_tag="VHH53_13855"
     CDS             complement(2611..4002)
                     /locus_tag="VHH53_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX2291245.1"
                     /translation="MPASAQPSPVPLSPSLVGQLLVAPTGRMPVMVHGATLADAQRAV
                     ADTGMQPVTTFRKIGVVAAWATGPQVQAARTQPGVTYIEGDQPIARYADAPQVATRGL
                     EARQTLVGANGSSLDGNSVSVAVIDSGIDPTHPAFAGSKVVRNLKSVCLDEANTDTNC
                     IVSLPTAVDTDTLSLGGHGTHVASIAAGRSVTLSDGNTVSGAAPEAKIISLSTGAAVA
                     VISADTALNWVLENHALPCGAGVSAAQCPPIKVVNNSYGPPGGGEFDENSAAVKLQRQ
                     LASEGVVTVWAAGNDGGDGSKSLTNPAAQDPTGGIISVAAYNDEGTGTRSGPVAGFSS
                     RGAKDDPSTWPDISAPGENIVGACRAYQPICSTGLKPQNGPGLLDVGSYNTLSGTSMA
                     APQITGIVALLFQINPTATPAEIEAALKSTAFHYSDGAPYQNVGGYATSFDKGTGLVD
                     VVAAAQTLGAHTP"
BASE COUNT          683 a         1507 c         1524 g          729 t
ORIGIN      
        1 ctgttcgacc ctggcaccct ggccgagctg gcaccccgtg atgccagcgg tacgttcgcc
       61 gcccgcggcc ccatcgacgg gaccacggcg atcgcctact gcaccgatgc caccacgatg
      121 ggcggtgcaa tggggtggca aggctgccag cacatcgtga cagccatcga taccgcgatc
      181 cagcaacgtt gcccggtgat cgggttgtgg cattccggcg gggcgcggct ggccgagggt
      241 gtcgaggctc tcgacgccgt cggccaggtg ttctccgcga tggtccgtgc ctccgggcgg
      301 gtgccgcaaa tctcggtagt gctcggggcc gctgccggcg gggccgccta cggtcctgcc
      361 ctcaccgata tcgtgatcat ggcgccggag gggcgggtgt ttgtcaccgg tcccgacgtc
      421 gtccgcagcg tgaccggcga gcagatcgac atggagtcct tgggcggtcc cagcgcacat
      481 ggcaagaaat ccggcgtcgt gcacgtgatg gccgactccg agtccgacgc gctgctgcgt
      541 gctcgccata tcgccggcct gctcgcccgg cctggaatgt tcgacctgga ctcggtacgc
      601 gaggaggtcg acctctccga acaccttccc gcggagcagc aacgggctta tgacgtccgc
      661 ccgttggtgc gtgccgtgct ggaccagccc gaccccaatc ctgacgccgg ggctgacact
      721 tcatctgatc cggccggtgg gttcgtggag ctgcagccca aatgggcacc caatgtcgtg
      781 gtgggactgg gcaggctcgc cgggcgcacc gtcggcgtga tcgcgaacaa cccgttgcga
      841 ctcgggggct gcctggactc ggtatcggcc gagaaggcgt cgcgctttgt gcggatgtgc
      901 gactcgttcg gggttcccct cctggtcctg gtggacgtgc ccggttacct gcccggtgtc
      961 ggccaggagt gggacggcgt ggtgcgtcgc ggcgccaagc tgctgcacgc cttcgccgag
     1021 gcggtctttc cccgggtcac cctggtcacc cggaaagcct tcggtggtgc ctacatcgcg
     1081 atgaactccc ggtcgctggg agctactcag gtcttcgcct ggcccgacgc tcaggttgcc
     1141 gtgatgggcg ccaaggccgc agtgggcatc ctccatcgca aagcgctggc cgcggcagca
     1201 caggaggagc gggaggcgtt gcatgccaaa ctcgccgagc accatgagcg gatcgccggg
     1261 ggcgttgatc gcgcggtggc gatcggcgtg gtggacgagg tcgtcaagcc ggcgcagacc
     1321 cggcgacgcc tggcggaagc gttggcggcg gcaccggaga gccgcggcgg gcacggcaac
     1381 atcccgctgt agttggtcag cagggcaccc gcaggcttgt gctaggcgag ctcgggctca
     1441 gtcgtagttc gcctgcatcg ccgatcagcg gataggacac ccaccaccgc aagcaccgca
     1501 ccggcatgtc gggcggcgcg tcggcagggt cctgcaagct ggtgaacgac agcagggcga
     1561 ccagcccgcc accagctcgg ggttccaacc ggtgcagcac gatactgccg tcgagggtgg
     1621 accggtactg gtcgcgccag gcggcttccc cggtgtcgcg ggcgtgctgc ggggtgacgg
     1681 tgctgcgcca cagcttgtaa tcgcctgcgt tgatcgcgtc gaaatacttc tgcagtaccc
     1741 ggcgtacccg gttggcgtcg ggatgcaccg cgacgtcctc gaccagcgcg acggtggtca
     1801 gcccaggccc cggtgcgggc gcatgggacg gtgcgttgtg ggcgatcgac gcagcgccgt
     1861 cggcgctagc ggtgggctgc agctggcgca ccagcacccc ggcccctact gcggcggcga
     1921 gtaccgccac tgtcaccgca cacaaagccc accggcgtgg aacgacacgc tgcaccggcg
     1981 caggttacgc ccctgctcag cccacgcggt gcagccaggt caccggagca ccgtcggcag
     2041 cgcggcggaa cggctccagc gcctcgtccc acgctgcacc gagaagctcg tcgagcaggt
     2101 gggcgaaggt ctctccgctg cgagcattgg cggccagtgc ccgcaactga tcctcgccga
     2161 ccacgatgtc accgttggca ctggtgcggg cacgccacaa accgagccca ggcacgaagc
     2221 agaaccgctc tccgtccacc cccgggctgg gttgctcggt cacttcgaag cgcaacatgg
     2281 gccacgctcg cagcgcgttg accacagccg ctgcgacacc cggttggccg gaccagtcgc
     2341 attcggcgcg cagctgccca ggcgcggccg cctgagcagt ccactgcagg tcggccctgg
     2401 tctgcagggc gcccgctatt gcccactcga cgtgcggaca taccgccgac ggcgacgagt
     2461 ggacgtagat agtcccactg gtgctcatct ggtgacctcc gctgatcgac gagaagcgtc
     2521 ttcccctacg tcctcgagat caaccggtgc accgtcacac attgtgccct tacggccgct
     2581 ggcacgccag tagccggggt gtgtcgtggc tcagggcgta tgtgccccca acgtttgcgc
     2641 ggcagcaacc acgtcgacca atccggtgcc cttgtcgaag gatgtggcgt acccacccac
     2701 gttctgatag ggcgcgccgt cggaatagtg gaaggcagtc gacttcaacg cggcctcaat
     2761 ctcagccggc gtcgcggtcg gattgatctg gaagagcaac gccacgattc cggtgatctg
     2821 cggtgccgcc atcgacgtac cgctgagcgt gttgtagctg cccacgtcga gaagcccagg
     2881 gccgttctgt ggcttgagcc cggtggagca gatcggctgg taagcgcgac acgcgccgac
     2941 gatgttctcg ccgggtgccg agatatccgg ccaggtcgag ggatcgtcct tggcgcctcg
     3001 ggaggagaat ccggctaccg gcccgctgcg ggttccggtg ccctcgtcgt tgtaggcggc
     3061 caccgagatg atcccgccgg tggggtcctg agcggccggg ttggtgaggc tcttcgagcc
     3121 gtcgccgccg tcattgccgg cggcccagac ggtcaccact ccctccgacg cgagctggcg
     3181 ctgaagcttc acagcggccg aattctcgtc gaactcgccc ccgccaggcg ggccgtagga
     3241 attgttcacc accttgatcg gcgggcattg cgcagcagac actcccgctc cgcacggcag
     3301 ggcgtggttt tccagcaccc agttcagcgc cgtgtccgca ctgatcaccg ccacggccgc
     3361 gccggtggac aaggagatga tcttggcttc aggcgcggct ccggagactg tgttcccatc
     3421 actgagtgtg acgctgcgac cagccgcaat gctcgccacg tgcgtgccgt ggccgccgag
     3481 ggacagcgtg tcggtgtcga cagcggtggg cagcgacacg atgcagttgg tgtcggtgtt
     3541 ggcttcatcc aggcagacgc tcttcagatt tcgcaccacc ttgctcccag cgaatgccgg
     3601 gtgcgtgggg tcgattccgg aatcgatcac ggccacgctg acgctgtttc cgtccagcga
     3661 ggagccgttg gcgccgacca aggtctgccg ggcctccagc ccgcgggtcg ccacctgagg
     3721 ggcatcggcg taccgcgcga tgggctggtc gccctcgatg taggtcacac cgggctgggt
     3781 gcgtgccgcc tgcacctgtg gcccggtcgc ccacgcggcc accacgccga tcttgcggaa
     3841 ggtggtgacc ggctgcatcc cggtatcggc gaccgcgcgt tgggcgtcgg caagcgtcgc
     3901 tccatgcacc atcaccggca tccggccggt gggagcgacg agcaactggc cgaccaatga
     3961 cggtgacagc ggcacaggtg atggttgggc actcgccggc atggcggtca gcgaaccggc
     4021 agcgagcaga ccggacacca gcagaccggt cagcaatccc cgtcgagtca tgacctcccc
     4081 ttcgtcgtac ctgagcgatg catctgggcc aaccgataag agtgaacacg tagcgcgcac
     4141 aatcgtcaac ggagcgtatc ggtgtcgcta cggtgccccg acacgatggc aacactgagc
     4201 gagacgcctc cggccccgat cggttgcttc cctccgcgtg tccgcacgct gtgtatgcat
     4261 gcgcgcgcgc tatgtacaga agtcgtcagc ctcgtgagtg tccggccgcc agggtggcca
     4321 ccggcagcac tgtggcgccg agcactccga gcccgactgt gagggcgatc cccactgcgc
     4381 cggccagcgc ttcgtcgatg cccagtagcc ggtccaccga ggcggtgccc ggaccgagtg
     4441 acc
//
LOCUS       DATADN010001078         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1196341, whole genome shotgun sequence.
ACCESSION   DATADN010001078 DATADN010000000
VERSION     DATADN010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196341"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..853)
                     /locus_tag="VHH53_13860"
                     /pseudo
     CDS             complement(1..853)
                     /locus_tag="VHH53_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
     gene            882..>1862
                     /locus_tag="VHH53_13865"
     CDS             882..>1862
                     /locus_tag="VHH53_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX2291246.1"
                     /translation="MSRLRLPTLELSAGLLGRLHRLADVSGSAGVMYARISEDREGAG
                     LGVERQLEDQCVLFGQLGLRLAGVYADNDLSAFTGKPRPDYLAMLADLDAARARVVTA
                     WHTDRLHRSPKELETYIDLAERRGVVTHSVKAGPLDLASPSGRAVARTLCAWARFESE
                     HKGERVRAARRQAAEQGRWQGGCRPFGFEPDGTTIRPAEAAAIAHASEAIVAGASLRS
                     IVRDLNDAGHHTVWGHTWTSVAVRDMLRRPRNAGLTSYGTETFPATWPAIVPETTWRA
                     VVSILSNPTRRTNTSGSNKVKWLGSGLYVCGACGQPALRVSYIGNHRHPAY"
BASE COUNT          295 a          630 c          649 g          288 t
ORIGIN      
        1 acatgccctc acgtagttcg cgagggagct gtccgaggtg ctggtgcgcc agcgtcatcg
       61 cgacccggaa cccgcgggct tcggcgagca tgtcctcgat cgggtacggc aggttcaaga
      121 agttgtggca ctcgtcgatc accagcgagg cgtcgcggcg ttggcgttgc ggggtacgag
      181 cgcggccggt ggtggcttgc cacgtcctgg ccaccacgag cgacccgact aacctggtgg
      241 tctcttcacc cagtgatccc ttgggtatgc gcaccaggca gatcccgccg tcgagcacgg
      301 cggtcatgtc cactgtggag tgaccacctg cgatggcgtc ccgaacgaac gggcggagca
      361 ggaacgcgcg cagcttgttc atcagtgggc tgatcacctg cgaccgcgac gagtcggtga
      421 gttcggcgta ccaggaccag aaaccgcgca ggaccgggtc ggtgatcccg gccgtcaccc
      481 gtgagcggaa tgcggggtcg gcgagcagct tcggcaggtc ggcgagggtg gcgacgcctt
      541 cctgggtgcg tagcgtcaag caggcggcgc gcatcacgtc gtcggtgcgc ggaccccaga
      601 acgcggagta cacccgacga aagacactga ccaggttgtc gacggtcagg tcggtctccc
      661 cgccgtcgag cggattcagg cagggtgggc ggctgttgct gtcggcgtcg aacagcacga
      721 cccgatccgc cgccgagcgc ggcagccggg agagcacgtc ggtgaccagg tcgcccttgg
      781 gatcgatgag cacgatcccg cgcccggctt cggcatcggc caggatcatg ttgcccagca
      841 aggtcgactt cccgaggctg tctctgtgac acacttaatc catgagccgt cttcgactgc
      901 caactttgga gctgtccgcc gggttgctgg ggcgcctgca ccggttagcg gacgtgagcg
      961 ggtcagcggg ggtgatgtat gcgcggatct cggaggatcg ggagggcgcg gggctaggtg
     1021 tggagcgcca gctagaggat caatgtgtgc tgttcggtca gctcggcttg cgcctggcgg
     1081 gcgtgtacgc cgataacgac ctctccgcgt tcaccggcaa accccgcccg gattacctgg
     1141 cgatgctggc cgatctcgac gcggcccggg cccgggtggt cacggcgtgg cataccgacc
     1201 ggctgcaccg cagccccaag gaactggaaa cctacatcga ccttgccgag cggcgtgggg
     1261 tcgtgacgca ctcggtgaag gcgggcccgc tggatctggc gagtccctcc ggccgggcgg
     1321 tggcccgtac gttgtgcgcg tgggcccgat tcgaaagtga gcacaagggc gaacgggtcc
     1381 gcgccgcccg gcgccaggcc gccgaacagg gccgctggca gggcgggtgc cgacccttcg
     1441 ggttcgagcc ggatggcacc accatccgcc ccgctgaggc cgccgccatc gcccacgcca
     1501 gcgaggccat cgtggccggg gccagcctgc gcagcatcgt gcgcgacctc aatgacgcgg
     1561 gccatcacac cgtctggggc catacctgga cctccgtcgc tgttcgggac atgctgcgcc
     1621 gacctcgcaa cgccgggctc accagctacg gcaccgagac cttccccgcc acctggcccg
     1681 ccatcgtccc ggagaccacc tggcgcgcgg tggtgtccat cctcagcaac cccacccgac
     1741 gcaccaacac ctccggcagc aacaaggtca agtggctggg cagcggactg tacgtgtgcg
     1801 gcgcttgcgg ccagcccgcg ctgcgggtct cctacatcgg caaccaccgc caccccgcct
     1861 ac
//
LOCUS       DATADN010001079         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_589552, whole genome shotgun sequence.
ACCESSION   DATADN010001079 DATADN010000000
VERSION     DATADN010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589552"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1117)
                     /gene="pheS"
                     /locus_tag="VHH53_13870"
     CDS             complement(56..1117)
                     /gene="pheS"
                     /locus_tag="VHH53_13870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX2291247.1"
                     /translation="MSGTNDSYDPKQVAVLAPEVLAQAVEDAEKAFAQAADLDELAAV
                     KPAHLGDRSPLLTARREIGALPPQARAEAGKRLNSSRTAVQAAFDQRRAALAAERDTG
                     KLRAEAVDVTLPWDRVPEGARHPITTLCERIADVFVAMGYEVAEGPELEAEWFNFDAL
                     NFPQDHPARTMQDSFYVAPEGSGLVLRTHTSPVQIRTLLARELPVYVVCPGRTFRSDA
                     LDATHTPVFHQVEGLVVDKGITMAHLKGTLDAFARAMFGHTSRTRLRPSFFPFTEPSA
                     EVDVWFPERKGGAGWVEWGGCGMVNPNVLRACGIDPEVHSGFAFGMGLERTLMFRNGI
                     PDMRDMVEGDVRFSRPFGI"
     gene            complement(1178..>1602)
                     /locus_tag="VHH53_13875"
     CDS             complement(1178..>1602)
                     /locus_tag="VHH53_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2291248.1"
                     /translation="AVLFAGNTVDPHNGKCVRASTGSLFHLPLGVEPDPQRAVAACRQ
                     AGLQVLAADGRTGRDLDDVIDAGTLAMPTAWLFGSEAQGLDRALLAAADGAVAVPLHG
                     PAESLNLASAAAVCLYASARAHRRRLAPGRDHLGSGEN"
BASE COUNT          254 a          551 c          567 g          230 t
ORIGIN      
        1 cgagccagga ctgcggaaca cgcacttctc gccctcccgg gcagactaga ggccgtcaga
       61 taccgaacgg gcggctgaac cgcacgtcac cctcgaccat gtcgcgcatg tcggggatgc
      121 cgttgcggaa catcagggtg cgctccagac ccatgccgaa ggcgaacccg gagtggacct
      181 ccgggtcgat gccgcaggcc cgcaggacgt tggggttgac catcccgcag ccgccccact
      241 cgacccagcc ggcaccgccc ttacgctccg ggaaccagac atcgacctcg gcggacggtt
      301 cggtgaacgg gaagaacgag ggccgcaacc gggtccggga ggtgtggccg aacatcgctc
      361 gagcgaaggc gtccagggtg cccttcaagt gagccatggt gatgcccttg tccaccacca
      421 ggccctcgac ctggtggaag accggagtat gggtggcgtc cagcgcgtcg ctgcggaaag
      481 tccgtcccgg gcacaccacg tacaccggta gctcccgcgc gagcagggtg cgtatctgca
      541 ccggggaggt gtgcgtgcgc agcaccaagc ccgacccctc gggcgccacg tagaagctgt
      601 cctgcatggt gcgggccggg tggtcctggg ggaagttgag cgcgtcgaag ttgaaccact
      661 cggcctcgag ttcagggccc tcggccacct cgtagcccat cgcgacgaac acgtcggcga
      721 tgcgctcgca gagcgtggtg atcggatgcc gggcgccttc cgggacccga tcccacggca
      781 atgtgacgtc aacggcctcg gcccgcagct tgccggtgtc ccgctcggcc gccaatgcgg
      841 cgcggcgctg atcgaaggct gcctgcacgg ccgtgcggct ggagttgagc cgcttgccgg
      901 cctcggcgcg agcctgcggt gggagcgcac cgatctcgcg gcgggcggtc agcaacggcg
      961 accggtcacc gaggtgggcg ggtttgaccg cggccagctc gtcgaggtcg gcggcttggg
     1021 cgaaggcctt ctcggcgtcc tcaacggcct gggccagcac ttctggagcc agcacggcga
     1081 cctgcttggg atcgtaggag tcgttggttc cagacatgag aggaactcct gggcttccgg
     1141 cgaccagcga cgtcaggcct gccgtgccgg tattcggtca gttctcgcca gatcctaggt
     1201 gatcccggcc gggggcaagg cgccgacggt gcgcccgcgc gctggcatac aaacagaccg
     1261 ccgccgcgga cgccaggttg aggctctccg cgggcccatg cagcggcaca gcgacagccc
     1321 cgtcggctgc cgcgagcagt gcacggtcca gtccctgcgc ctcactgccg aacagccagg
     1381 cggtcggcat cgccagagtt ccggcgtcaa tcacgtcatc gaggtcgcgg ccggtgcggc
     1441 catcggctgc caggacctgc aacccggcct gccggcacgc cgccacggcc cgctgcggat
     1501 cgggctcgac gcccagcggg aggtggaaca ggctaccggt ggaggcccgc acgcatttgc
     1561 cgttgtgcgg gtcgacagtg ttcccggcga agagcaccgc gc
//
LOCUS       DATADN010001080         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_274616, whole genome shotgun sequence.
ACCESSION   DATADN010001080 DATADN010000000
VERSION     DATADN010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274616"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            831..1112
                     /locus_tag="VHH53_13880"
     CDS             831..1112
                     /locus_tag="VHH53_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291249.1"
                     /translation="MTLSFAEIDSQHVELLPARTVMSLFTLGDPGINGSGGDGGDGKG
                     GPVACAVSIGDLTAALGLILGSGSAEGSGSSCNSTGTGGAGGTGVFGGK"
BASE COUNT          300 a          482 c          498 g          313 t
ORIGIN      
        1 taggggctga tgctgcccat cggcctgatc gtgtcatcga tgtaggcggc ccgggcaggg
       61 gacacgtgat ctggtcgcta cagcgtcgaa gatgaccggg ggcggcgccc acattatcca
      121 ccagctccca gataggacag agtgtgcaat ccaccagcac cgtttcacgc caacgatgcc
      181 gccggctggg gcacgaactc gccgagcacc ctgattacga agatcgcacc ggcgaactca
      241 tgagtcacgt tcttatgcat caaacacacg atcagcgcca ctgaccggaa cagccgcagc
      301 gtgtagggac gcggccgtcg gtgagttact catcgcgcac gccggccata gcctcatgcc
      361 gcagcggctc atccgatttc acttcagctg tctggtcgac aacggaataa cacggcaggg
      421 agtcgttgcc tttgccaagc gtgacacgtg ctattcagag actgtggagc aattgtgagc
      481 tggcttcccg cggagaagcc gtcgggctca ctcaagcgtt atcctctcat gcggcttgcg
      541 aattaatctt ccggtacttg ttgcaccggc tggctccagc tcacatgcct acccgcgccc
      601 cgtgtgacct cgcgcagtga gtagtcggcg gcgggatcca cgtaactgct cgccgccagc
      661 taagtattcg aaagtcgctt caacacgtag cggcatgcga aagtttaggt ggcgaatgca
      721 tgttgtcagg tgaccttttc caagccatgg tagttaataa ccgaatcggc aatgccgtcg
      781 atgaggcgta atgtttccgg cccccttggg ccctgtgtga ggagatctga atgactctga
      841 gctttgctga gatcgacagc cagcacgtgg agctgctgcc ggcgcgcacg gtcatgtccc
      901 tgttcaccct cggtgatccc ggcatcaacg gaagtggtgg cgacggcggc gacggtaagg
      961 gcggtcccgt cgcctgtgcg gtcagcatcg gcgacctcac tgccgctctc ggtttgatcc
     1021 ttggcagcgg ttccgccgag ggcagtggca gctcgtgcaa cagcaccgga accggcggcg
     1081 ccggcggcac tggcgtattt ggtggcaagt aacaccgagc gagcgtgaga taaatcggtg
     1141 ttcgcgtgtg gtgccgttgg ctagggaatg gcaccggcat ggccgacgca accgcccagg
     1201 gtggtgctgg ggcagccgac tgcagtgggt cgtgcagtac ccccacgtgg tcagatgacc
     1261 gcgtgcggtg ccgttggcaa ctggaggggt ggccgcatca tggccacccc tcccgctatt
     1321 ttaccggcca cggcacgacg ccggtaaagt ccttatgccg ttgcgccagg ctggtcgttg
     1381 tgtaaatcca tttaccgcct cgcctgagta cgggctgatg ctgcccatcg tgcattgatg
     1441 tggacagccc ggcagcggac acgtgatcct ggtcgctgca acgtcgaaga tgacgaggac
     1501 agcgcccgca ttgtccaccc gctcactgat ctacgccgcg accaatacgc gtgtcgatga
     1561 cgcgattgct gggcgagcag tctccgccgc aaa
//
LOCUS       DATADN010001081         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_274653, whole genome shotgun sequence.
ACCESSION   DATADN010001081 DATADN010000000
VERSION     DATADN010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274653"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /gene="pspA"
                     /locus_tag="VHH53_13885"
     CDS             complement(<1..667)
                     /gene="pspA"
                     /locus_tag="VHH53_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEX2291250.1"
                     /translation="MANPFVKAWKYLMALFSSKVDEYADPKVQIQQAIEEAQRQHQAL
                     TQQAAQVIGNQRQLEMRLNRQLADIEKLQVNVRQALTLADQAVASSDAAKATEYNNAA
                     EAFAAQLVTAEQSVEDLKGLHDQALQAAGRAKKAVEQNAMMLQQKIAERTKLLSQLEQ
                     AKMQEQVSSSLRSMSELAAPGTTPSLDEVRQKIERRYANAIGEAELAQNSVQGRMLEV
                     QQAS"
     gene            complement(782..1102)
                     /gene="clgR"
                     /locus_tag="VHH53_13890"
     CDS             complement(782..1102)
                     /gene="clgR"
                     /locus_tag="VHH53_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ClgR"
                     /protein_id="HEX2291251.1"
                     /translation="MTALLREVIGDVLRRARIDQGRTLREVSDTARVSLGYLSEVERG
                     RKEASSELLSAICGALDIPLSRVLSDAGLEMEREERAAFAGAANIDATTKVVIPQVVS
                     MAVA"
     gene            complement(1239..>1587)
                     /locus_tag="VHH53_13895"
     CDS             complement(1239..>1587)
                     /locus_tag="VHH53_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2291252.1"
                     /translation="DPIADKALSGSALVGLSVLDIVPWWVTVVIMGREIGITVLRFAV
                     LRFGVIPASRGGKLKTLVQAVAIGLFVLPLSGAWLSSAWVIMWAAVVLTVVTGVDYVV
                     SAIKDSKARPADR"
BASE COUNT          237 a          553 c          514 g          283 t
ORIGIN      
        1 cgctggcctg ctggacctcg agcatgcggc cctgcaccga gttctgcgcc agctcggcct
       61 cgccaatcgc gttggcgtag cgacgctcga tcttctggcg cacctcgtcg aggctcggcg
      121 tggtgcccgg cgcggccagc tcactcatcg accgcagcga cgagctgacc tgctcctgca
      181 tcttggcctg ctcgagctgg gacagcagct tggtgcgctc ggcgatcttc tgctgcagca
      241 tcatcgcgtt ctgctcgacg gccttcttgg cccgcccggc ggcctgcagt gcctgatcat
      301 gcagcccctt gaggtcttcc acgctctgct cggcggtcac cagctgggcg gcgaacgcct
      361 cggcggcgtt gttgtactcg gtggccttcg cggcgtcact gctggccacc gcctggtcgg
      421 cgagcgtgag cgcctgccgg acattgacct gcagcttttc gatgtcggcg agttggcggt
      481 tgagccgcat ctccagctgg cgctggttac cgatcacctg cgcggcctgc tgcgtcagcg
      541 cctgatgctg gcgctgggct tcctcgatgg cctgctgaat ctgcaccttc gggtcggcgt
      601 attcgtcgac cttggagctg aacagcgcca tgaggtactt ccacgccttg acgaacggat
      661 tggccatgtc agttcactcc gccttcgtgt cgttgtctcg gactcgccgg cccggcttac
      721 cgtgctgccc aatctatcgg gtcagcgggc atcaccacac ccactcggtg ccggaccagg
      781 atcaggcgac cgccatcgac acgacctgcg ggatgaccac cttggtggtg gcgtcgatgt
      841 tggcggcgcc cgcgaacgcg gcacgctcct cgcgctccat ctccagaccg gcgtcggaga
      901 gcacccgcga cagcgggatg tcgagagcgc cgcagatcgc gctgagcagt tcgctggagg
      961 cttccttgcg cccgcgttcg acctcggaga gatagccaag gctgacccgc gcagtatcgg
     1021 acacctcgcg cagggtgcgg ccctggtcga tgcgggcccg acgcagcacg tcgccgatca
     1081 cctcgcgaag caatgccgtc atcgtgctct ccctccgggg tcaatgctgg cccttttaaa
     1141 gggcggtgta ctagggcaac gccgcacgcg gggcggttgg ttcccagatg gcgcagatca
     1201 ctgtcgcgcg gcggtcgtat cgcgcggccg gcgtgctgtc agcgatcagc gggtcgggcc
     1261 ttggagtcct tgatcgccga aacgacgtag tcgacaccgg tgaccacagt gaggaccacg
     1321 gccgcccaca tgatgaccca ggccgaagac agccacgcac cggacagcgg cagcacgaac
     1381 aggccgatcg ccaccgcctg caccaaggtc ttgagcttgc cgccccggct ggcgggtatc
     1441 acgccgaacc gcaacaccgc gaaccgcagc acggtgatgc cgatctcccg ccccatgatc
     1501 acgacggtga cccaccaggg cacgatgtcc agcacggaca ggcccaccag tgcggagccg
     1561 ctgagcgcct tgtcggcgat ggggtcg
//
LOCUS       DATADN010001082         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1496901, whole genome shotgun sequence.
ACCESSION   DATADN010001082 DATADN010000000
VERSION     DATADN010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496901"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VHH53_13900"
     CDS             complement(<1..216)
                     /locus_tag="VHH53_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2291253.1"
                     /translation="MTLWRLHVDVADQPGQLGTLAAAIGGCGANIVSLHVIGEPGQDG
                     AVTDELLVKVPAQLPVRELVVAVEAAGF"
     gene            complement(280..>1752)
                     /locus_tag="VHH53_13905"
     CDS             complement(280..>1752)
                     /locus_tag="VHH53_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX2291254.1"
                     /translation="VGASTDEGTTVFISRDDAPAEMVYRSEHDAGVGSLSRDERLLAI
                     SHSEHGDSRHPAVRVLSTAGDTAAKTVAEKWDGPGKGLDVLSFSPIEGDARLLLTHER
                     RGRQELLIWDVAADTETEITLELPGEVVADWYPDATALLVVHNYSARSTLHRYDLGTG
                     ELTALDIPAGTIGDAGVRPDGTVEYAWSCAAEPPAIRAIHADGTDRILLRPESEPPPG
                     SEPLTDEFVPTLHGTVHALVARPGNAVGVPPQDPLPTVFLLHGGPQAADEDRFSAIRA
                     LWLDAGFAVVHVNYRGSTGYGSAWRDAIEGRPGLTELEDVAAVHDWAVRSGLADPTAC
                     VVEGWSWGGYLTLLALGTQPQRWTAGIAGIPVADYVTAYADEMEPLRAFDRALFGGSP
                     EEISDVYTASSPITYAAEVRAPVLVLAGENDPRCPIRQVDNYLARLAELGKPYRSYRY
                     DAGHGSLVVAETLRHVAAEVAFVREVLAASHPCVVEIAQR"
BASE COUNT          304 a          605 c          572 g          271 t
ORIGIN      
        1 gaacccggca gcctccacgg ccaccacgag ttcccgcacc ggaagctgcg ctggcacttt
       61 aaccagcagc tcatctgtca ctgcaccgtc ttgccccggc tcgccgataa cgtgcaggga
      121 aacgatgttg gctccgcaac cgccaatggc ggctgcgagc gtccccaact gtccgggctg
      181 atcggccaca tcgacgtgca gccgccacag ggtcacggtg acctcctccg gcttagagtc
      241 cgctcacggg gttaccaccg tgttgaccca cggtaacgtt caacgttgcg caatctccac
      301 cacgcaaggg tggctagcag cgagcacctc gcgaacgaag gcgacctcag cggcgacgtg
      361 tcgcagcgtc tcagccacta ccagtgaccc atgcccggcg tcgtaacggt aggagcggta
      421 aggcttaccc agctcagcga gccgagcgag gtagttgtcg acctgacgga tcgggcagcg
      481 cgggtcgttt tcccccgcca gcacgagaac gggagcccgc acctccgccg cgtacgtgat
      541 cggggacgac gctgtatata cgtcggatat ctcctcgggc gagccaccaa acagcgcccg
      601 gtcgaaggcc cgtagcggct ccatctcatc ggcgtaggcg gtcacgtagt cggccaccgg
      661 gataccggcg atccccgctg tccagcgctg cggttgagtt ccgagagcca acaacgtcag
      721 ataaccgccc catgaccagc cctctaccac gcatgcggtc gggtcagcca acccggagcg
      781 gaccgcccag tcgtgcacgg ccgcgacgtc ctccagctca gtcagccctg gccgaccctc
      841 gatcgcgtcc cgccaggccg agccgtagcc agtagaaccc cggtagttga cgtgcaccac
      901 ggcgaagccg gcgtccagcc agagtgcccg gatggcggaa aaccggtcct cgtcggccgc
      961 ctgcggacca ccgtgcagca ggaacaccgt gggcagtgga tcctgggggg gcacaccgac
     1021 ggcattgccc gggcgggcca ccagggcgtg caccgtcccg tgcagggttg gtacgaactc
     1081 gtcggtcaag ggttcggagc cgggcggagg ttcactctcg gggcgcagca gtatccgatc
     1141 ggtgccatcg gcgtgtattg cgcggatggc gggcggctcg gcggcgcagg accaggcgta
     1201 ctccacggtg ccgtccggcc ggacccccgc gtcaccgatc gtgccggccg ggatatcaag
     1261 ggcggtcagc tccccggtac ccaggtcgta gcggtgcagt gtggagcgtg ccgagtagtt
     1321 gtggacgacg agtagcgcgg tagcgtcggg ataccagtct gcgaccacct cgccgggcag
     1381 ctccagcgtg atctcagtct cggtgtccgc cgcaacgtcc cagatcagca gttcttggcg
     1441 gccacgccgc tcgtgggtga ggagcagcct tgcgtccccc tcgatgggag agaagctcaa
     1501 cacgtccaag cccttgccag gaccgtccca tttctcggcg actgtcttag cggcggtgtc
     1561 tccagcggtg ctgagcaccc gcaccgcagg atggcgggag tcaccatgct cggaatgcga
     1621 gatcgcgagc aaccgctcgt ccctggacag cgaccccact ccggcgtcgt gctcgctacg
     1681 gtagaccatc tcggcgggag cgtcgtcccg ggaaatgaac accgtggtgc cctcgtcggt
     1741 ggaggcgccg ac
//
LOCUS       DATADN010001083         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_736882, whole genome shotgun sequence.
ACCESSION   DATADN010001083 DATADN010000000
VERSION     DATADN010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736882"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VHH53_13910"
     CDS             complement(<1..212)
                     /locus_tag="VHH53_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291255.1"
                     /translation="MVEQSVRVRAGTAPRNLANLRDLRLPTADGTVKLSDVATVKQST
                     TPPSISHTDGSRSAEITARPAADDLGA"
     gene            250..1083
                     /locus_tag="VHH53_13915"
     CDS             250..1083
                     /locus_tag="VHH53_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2291256.1"
                     /translation="MLIRTGTAPGDLADLRELPLPTAGGTVELSDVATVKQSTTAPSI
                     SHTDGIRSAQITARPAADDLGAVTAALQVKLDELDLPAGATAEIGGVSADQSDAFAEL
                     TLALLVAIAVVYMVMVVTFRSLLQPLVLLVAVPFAATGALGLLLLTDTPLDVAGLIGM
                     LMLVGIVVTNAIVLIDRVNQYRRDGRPLFDAVVEGAAQRLRPILMTAVATVLALLPMA
                     VGFTGGGVFISQPLAIVVIGGLVSSTLLTLVLVPVLYLLTEPKRGRHRRRRVEAALQP
                     G"
     gene            1148..1639
                     /locus_tag="VHH53_13920"
     CDS             1148..1639
                     /locus_tag="VHH53_13920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2291257.1"
                     /translation="MSMLDDLLVRVLRGPMQRLMAEERNRFTYGDSEDPLYRYQVHGA
                     LERLHIDPTAIVNNALFNVSGGEITIGEYAFFGHEVAVLTGTHDITKFGRERQTTYPR
                     SGRDVVIEEGVWVASHALVLGPVRIGKHAVVAAGALVLEDVAPYTVVAGRPAKVIKKI
                     DQQ"
BASE COUNT          289 a          589 c          608 g          320 t
ORIGIN      
        1 gcaccgaggt cgtcggcggc cggccgtgcg gtgatctcgg cgctgcggga accgtcggtg
       61 tggctgatgc tcggcggggt ggtcgactgc ttcaccgtcg cgacgtcgga cagcttcacg
      121 gtgccgtccg cggtcggcag ccgcaggtct cgcaggttcg cgagattacg gggggcggtc
      181 ccggcgcgca cccgcacgct ttgctccacc accgtcggca ccctgaccat tgacggggtg
      241 gaacaaagcg tgctgatacg taccgggacg gctcccggtg atctcgcgga cctgcgggaa
      301 ctgccgctgc cgaccgcggg cggcaccgtg gaactgtccg acgtcgcgac ggtgaagcag
      361 tcaaccaccg ctccgagcat cagccacacc gacggaattc gcagcgccca gatcaccgca
      421 cggccggccg ccgacgacct tggtgcggtc accgccgcgc tccaagtcaa gctcgacgag
      481 ctggatctcc cggccggcgc caccgccgag attggtgggg tcagcgccga tcagagtgac
      541 gcgttcgcgg aactcaccct ggccctgctg gtggcgatcg ccgtggtcta catggtcatg
      601 gtggtgacat tccgcagcct gctgcagccg ctggtgctgc tcgtcgcggt cccgttcgcg
      661 gccaccggtg cgctcggtct gttgctgctc accgatactc cgcttgatgt cgcggggctg
      721 atcgggatgt tgatgctcgt cgggatcgtg gtgaccaacg cgatcgtgct catcgaccgc
      781 gttaaccaat atcggcgcga tgggcgcccg ctattcgatg ctgtcgtgga aggagccgca
      841 cagcggctac ggcccatcct gatgactgcg gttgcgaccg tgttggcttt gcttccaatg
      901 gcggtggggt tcaccggcgg tggggtgttt atctcccagc cgctggccat cgtcgtgatc
      961 ggcggcttgg tcagctcgac gctgctgacc ctggtgctgg taccggtgct ctacctactc
     1021 actgaaccca agcgtgggcg gcaccgccgg cggagggtcg aggctgcatt acagccgggc
     1081 taaccgatcg agaagctacg tcggggtagc tccgaccagc ggtgctggcc caactagagt
     1141 ttcgatcatg tcaatgctcg atgatctgct ggtccgggta ctgcgcggcc cgatgcagcg
     1201 cctgatggcc gaagagcgca accgcttcac ctacggtgac agtgaggacc cgctctaccg
     1261 ctaccaagtg cacggcgcac tcgagcggct gcacatcgat cccacggcga tcgtgaacaa
     1321 cgcgctgttc aacgtgtccg gtggggagat caccatcggt gagtacgcct tcttcgggca
     1381 cgaggttgcg gtgctcaccg gcacccacga catcaccaag ttcggccggg agcgccagac
     1441 cacctacccc cgctccggtc gggacgtggt gatcgaggaa ggcgtctggg tagccagcca
     1501 cgcgctggta ctcgggccgg tgcgcatcgg caagcacgcg gtggtcgctg ccggtgccct
     1561 ggtgctggag gacgtggcgc cctacacggt cgtcgccggc cgccccgcga aggtgatcaa
     1621 aaagatcgac cagcagtgaa ggtcgagatc ggcgcggggg aaaagccgca tcccgattac
     1681 gacgtgcacg tcgacatcct cccgctaccc gatatcgaag tgatcagctg gattgaccgg
     1741 ttgccgttcg cgaccgcatc ggtcgacgcg ttgcgggcca accacgtgct cgagcaccaa
     1801 agctac
//
LOCUS       DATADN010001084         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_275430, whole genome shotgun sequence.
ACCESSION   DATADN010001084 DATADN010000000
VERSION     DATADN010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275430"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VHH53_13925"
     CDS             complement(<1..468)
                     /locus_tag="VHH53_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX2291258.1"
                     /translation="MANRRGWIRRLAAACWRHPYLSVAALAASGIGVGMEATIPLLTR
                     LAVDDAVAGSTQRLGWLVAALAGVALLRFGTAFVRRYLGGRLSLDVQHDLRRAVFRAV
                     QKLDGAKQDALRTGQVVSRAISDLQLVQGLLSLVPLVAGMLVLFVVALVAMLWL"
     gene            complement(551..1006)
                     /locus_tag="VHH53_13930"
     CDS             complement(551..1006)
                     /locus_tag="VHH53_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291259.1"
                     /translation="MDRRVRRAVLERLAAVEEMAARAGAALSVPVARNHIRTITDGWR
                     ELLVQHQPDPTGRCPVCSGWLRRRRWPCEVWETAHRHLIGDGTEPIERPAGKSGPFGR
                     PRQVLVVPRQLHAPAADQVTVNNLSRNRPDVTEPAIAMPDVRRGGYPRT"
     gene            1259..1450
                     /locus_tag="VHH53_13935"
     CDS             1259..1450
                     /locus_tag="VHH53_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX2291260.1"
                     /translation="MRVEVDLDICEGHGQCNAVAPEVYDLDDGGYCLIHNPEVTPELE
                     PHANDGALACPVQAITVTR"
     gene            1456..2139
                     /locus_tag="VHH53_13940"
     CDS             1456..2139
                     /locus_tag="VHH53_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2291261.1"
                     /translation="MPRPYVLVSVATSIDGYLDDMRQTRLLLSNTEDFDRVDAVRASV
                     DAILVGANTVRQDDPRLLVRSVERQEERIRRGLTRHPMKVTLTGRGDLDTAARFFSCG
                     DSDKLVYTRDSIVRVLHKALGDTATVVAAGEPIDLGAMLDDLARRKIKRLMVEGGGTV
                     VTQLLGAGFVDEIHLVIAPFFVGDASAPRFVHPGRFPHDVEHPMTLAETRQFGDIVLL
                     RYLATATGR"
     gene            2148..2597
                     /locus_tag="VHH53_13945"
     CDS             2148..2597
                     /locus_tag="VHH53_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEX2291262.1"
                     /translation="MIDRDRGHLQHAIELAQRCPVSPTAFSVGAVIIDAKGGVLATGY
                     SRETDPHDHAEEAALAKLASDDARLGKATLYSSLEPCSARASRPRSCTELILATPISR
                     IVFAWREPALFVDCQGAELLRAAGREVVELPELAHLVRHTNHHLLES"
BASE COUNT          464 a          973 c          907 g          445 t
ORIGIN      
        1 cagccagagc atggcgacca gcgcgacgac gaacagcacc agcatgcctg cgaccagcgg
       61 caccagcgac agcagaccct gtaccagctg caggtcggag atcgcgcggg acacgacctg
      121 cccggtgcgg agggcgtcct gcttggcgcc gtcgagcttc tgcaccgcgc ggaacacggc
      181 gcggcgcagg tcgtgttgca cgtccaggga tagccgcccg ccgaggtagc ggcgcacgaa
      241 ggcggtcccg aagcgcagta gcgccacccc ggccagcgcg gcgaccagcc agcccagccg
      301 ctgggtgctg cccgccaccg cgtcgtccac agcgagccgg gtcagcagcg ggatggtggc
      361 ttccatgccg accccgatcc cggaagcggc cagtgccgcc acactgagat agggatggcg
      421 ccaacatgca gccgcgagcc tgcggatcca gccgcgtcgg ttcgccatgg tctgctcggg
      481 cacgaccgtc agattaggcg gccactccga caaactgcgc cgggctcagc accgccgggt
      541 ggaaggacga tcaagtgcgg gggtatccac ctcgccgcac gtcgggcatc gcgatcgcgg
      601 gctcggtcac gtcgggccgg ttgcgggaaa ggttgttgac ggtgacctga tcggcggcag
      661 gagcatggag ctggcgcggg accaccagca cctggcgggg tcggccgaac ggaccggatt
      721 tcccagcggg gcgctcgatc ggctcggttc cgtcaccgat gaggtgccgg tgcgcggtct
      781 cccacacctc gcagggccag cgacggcggc gaagccaccc tgagcacacc gggcagcgac
      841 cagtggggtc cggctgatgt tggacgagca gttcccgcca tccgtcggta atggtccgga
      901 tgtggttgcg cgctaccggt acagacagcg ccgcacccgc ccgagcggcc atctcctcca
      961 ccgccgccaa tcgttccaac accgcacgcc gcaccctgcg gtccaccagc ccacctcctc
     1021 gtcgcccccc gaccatcccg agagcgcacc tgacacgcat aacgacttgg cccaaacgcc
     1081 gtcacgcctc aaggcattgt tctaccggac ttcgctcgtg gacttaaccc cccctcgcac
     1141 tcgtcacccc ttcaccgatc cgtacgccgc gcttaccctc caggcgtgtg cgctgtggcc
     1201 ggcgagacca aaatggttga gttccaccac gctgagccca caagccgagg aggacgccat
     1261 gcgcgtcgag gtggacctgg acatctgcga gggccacgga cagtgcaacg cggtcgcacc
     1321 cgaggtctat gacctcgacg acggcgggta ctgcttgatc cacaatccgg aagtcacacc
     1381 cgagctggag ccccatgcca acgacggggc gctggcctgc ccggtgcagg ccatcaccgt
     1441 cacgcgctga cagccttgcc ccgcccgtac gttctcgtct cggtggcgac gtccatcgac
     1501 ggctacctcg acgacatgag acagacgcgg ttgctgctgt ccaacaccga agatttcgac
     1561 cgcgtcgatg cggtacgcgc cagcgtagac gccatcctgg tcggggcgaa cacggtccgc
     1621 caggacgacc cgaggttgct tgtccgctcc gtggagcgtc aggaggagcg aatccgacgc
     1681 ggcctgactc ggcatcctat gaaggtcacc ctgactggcc gcggggatct ggacactgct
     1741 gcacggttct tctcctgtgg tgacagcgac aagctcgttt acacccgtga ttcgatagtc
     1801 cgggtgcttc ataaggcgct gggggatacc gcaacggtgg tggcagccgg agagccgatc
     1861 gatttgggtg ccatgctgga cgatctggcg cggcgcaaga tcaagcgcct gatggtcgaa
     1921 ggtggtggca ccgttgtcac ccagttgctg ggcgcaggct tcgtggacga gattcatctc
     1981 gtgattgcac cgttcttcgt gggtgacgcc agtgcgcccc gcttcgtgca tcccggtcgg
     2041 ttcccgcacg atgtcgagca tccgatgacc cttgccgaga cccgtcagtt cggggacatc
     2101 gtcctgctcc gttacctcgc cactgccacc ggccgatgac agctcccatg attgatcgcg
     2161 accgtgggca cctccagcac gcgatcgagc ttgcgcagcg ctgccccgtt tcgccaaccg
     2221 ccttctcggt aggcgcggtg atcatcgacg ctaagggcgg cgtgctcgcc acgggctact
     2281 cccgcgagac cgatccccac gaccatgccg aggaggctgc acttgccaag ctcgcctctg
     2341 acgatgcccg gctcggcaaa gccaccctct acagttcgct cgaaccgtgc agcgcccggg
     2401 cctcacgacc gcgcagctgc actgagctca tcctcgccac tccgatctcg cggatcgtgt
     2461 tcgcctggcg cgagcccgcc cttttcgtcg actgccaggg cgccgaactc ctccgcgccg
     2521 ccggtaggga agtcgtggag ctacccgagc tcgcccacct ggtgcgccat accaatcacc
     2581 acctgcttga gtcctgagcg gacgctcgat agtgggtcag ctgggccgga aggtaggtaa
     2641 tggggagcag cgtcgcgcaa gctggtgatg tggccagatc aacggctggg tggcggggcg
     2701 ctggtgtggt gaccgcgctg ggagcgctcg cggctggctg tggggtgcag gtgccatatg
     2761 cttccgcgcc ccccgcggtg accgatggc
//
LOCUS       DATADN010001085         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1497186, whole genome shotgun sequence.
ACCESSION   DATADN010001085 DATADN010000000
VERSION     DATADN010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497186"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(556..1560)
                     /locus_tag="VHH53_13950"
     CDS             complement(556..1560)
                     /locus_tag="VHH53_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX2291263.1"
                     /translation="MRLGVLDVGSNTVHLLVVDAHRGGHPMPMISEKSVLRLAEHMDA
                     RGKIRPEGADHLIRTVRAAANSASAMGCADLMAFATSAVRDASNVGAVLARVQAETGI
                     ELEVLSGKQEAILTFLAVRRWCGWSAGRVLMLDIGGGSLEIASGIDEEPDVAVSLPLG
                     AGRLTRSWLAHNPPRRRELANLREYIAEQLEEPARKLRKADASDQVVGTSKTFRSLAR
                     LTGAAPSSAGLRVRRTLTDAGLRQVIAFISRMSSDDLAELEGVSASRSHQLVAGALVA
                     QAAMHALDVDELSLCPWALREGVILQKLDWLDTESTGRRSRGAAHLDVLDETAARGAR
                     "
     gene            complement(1604..2293)
                     /locus_tag="VHH53_13955"
     CDS             complement(1604..2293)
                     /locus_tag="VHH53_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2291264.1"
                     /translation="MTRVLIVEDEESFADPLAFLLRKEGFSTAVATTGSAALEEFDRN
                     GADIVLLDLMLPGMSGTDVCKQLRARSAVPVIMVTARDSEIDKVVGLELGADDYITKP
                     YSARELIARIRAVLRRGGDIEGDGALGPSVLEAGPVRMDVERHTVTVAGQEIGLPLKE
                     FDLLEYLLRNVGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKIERDPSSPQHLV
                     TVRGLGYKYDS"
     gene            complement(2290..>2996)
                     /locus_tag="VHH53_13960"
     CDS             complement(2290..>2996)
                     /locus_tag="VHH53_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2291265.1"
                     /translation="HELKTPVGAMALLAEAVLDATDDPHEVRRFGTKILNEATRLGAL
                     VTELIALSRLQGAERLPELATVEVDEVVREALDRCRLAAESADIRITVDPPSGLLLDG
                     DATLLVTALSNLVDNAVSYSPPGSPVSVSRRLADGFVEIAVTDRGLGIAAEHQERVFE
                     RFFRIDQARSRATGGTGLGLAIVKHVAANHGGDVQLWSRPGTGSTFTLRVPAHPQSAE
                     RIPHTPPTPEHVGGSL"
BASE COUNT          500 a         1040 c          976 g          480 t
ORIGIN      
        1 gccaggccag ggagcggggt ccgtgggcgc cggtgcgact cgggggaact ggacggtggc
       61 cggatcgccg gacagcaccg tcgcgctgag tgggctaagg gagctaagtg accggtgtcc
      121 ggcaatcacc gatggtcggc ccaccgctga ggtccctacc gcaggggtcc cccctggggg
      181 agttcgcacg gcggtgcttt gcacggccgg gggccgcacg ggtgtggtgc gcaccgccgg
      241 tgtctctacg gccggtgccc actgccggcc accgccaccg ttgccggatg acagggatga
      301 ataccgctga gacggtccat gatgactgga tggggcggca agaggcggcg cgccgccgcc
      361 aggatctgtc cgggtggtcc gggtcggttc gacggggggc tggacaggag tgttcacacc
      421 gacgccggtg tcgtcgtcgg cagctcggcg gcgacgacgc ccggtattcg gggtcggctc
      481 accatagcga gccaccagct cagccacgct gaactgggcc cgctcttcag gctcgctgct
      541 gcgcctcatc gtctctcacc gtgccccccg agccgctgtt tcgtccagta cgtcgaggtg
      601 ggcggcgccc cggctgcgcc gtccggtcga ttccgtatcc aaccagtcca gcttttgcag
      661 aatcacacct tcccgcaacg cccacgggca tagcgacagc tcatcgacat ccagagcgtg
      721 catcgcggcc tgggccacca acgctccagc gacgagctga tgggaccggc tggcgctgac
      781 accctccagt tccgccaggt cgtcagagct catccgggag atgaacgcga tcacttggcg
      841 tagaccggca tcggtcagcg tacggcggac ccgcagcccg gctgacgagg gggcggcacc
      901 ggtgagccgg gccagcgatc gaaaggtctt cgaggtgccc accacttgat ctgaagcgtc
      961 ggccttgcgc agcttgcggg ctggctcctc gagctgctcg gcgatgtact cgcgcaggtt
     1021 cgccagctcc cggcgacggg gcggattgtg cgccagccag gaccgagtga gccggccagc
     1081 gccgagcggc agtgataccg ccacatccgg ctcttcgtcg ataccactgg cgatctccag
     1141 ggatccgccg ccgatgtcca gcatgagcac ccggccagct gaccagccgc accaccggcg
     1201 gaccgcaagg aaggtgagga tggcctcctg cttaccagag agcacctcga gctcaatccc
     1261 ggtttcggcc tgcacccggg cgagcaccgc accgacgttg gaggcgtcgc gcaccgccga
     1321 ggtggcgaag gccatcaggt ccgcgcagcc catcgcggac gccgagttgg cggcggcacg
     1381 cacggtgcgg atcaagtgat cggcaccctc aggcctgatc tttccacggg cgtccatgtg
     1441 ctcggccagc cgcaacaccg acttctctga aatcatcggc atcggatgcc cgccacggtg
     1501 cgcgtccacc accagcaggt ggacggtgtt ggagcccaca tcgagcacgc cgagccgcac
     1561 ggcaggtcag ggtacctgcc ttagatatcg tctgtcctaa atgtcaggag tcgtacttgt
     1621 aacccagccc gcggacggtc accaggtgct ggggcgacga cggatcccgc tcgatcttgg
     1681 accgcaaccg cttgacgtgt acatcgaggg tcttggtgtc gccgacatag tcggctcccc
     1741 atacccggtc gatgagctgg ccacgggtca gcacccgccc tacgttgcgc agcaggtact
     1801 ccagcaggtc gaattccttg agcggcaacc cgatctcctg cccggcgacg gtgacggtgt
     1861 gccgctcaac atccatccgc accggaccag cctcaagcac cgaaggtccg agggcaccat
     1921 ccccctcgat gtctccaccg cggcgtaaca ccgcacggat ccgagcgatg agctcgcgag
     1981 ccgagtacgg cttggtgatg tagtcgtcgg cacccaactc cagaccgacc accttgtcga
     2041 tctcgctgtc ccgagcggtt accatgatca ccggtacggc ggagcgggcc cgcagttgct
     2101 tgcagacgtc ggtgccgctc atcccgggga gcatcaggtc gagcagcacg atgtcggcac
     2161 cgttgcgatc gaactcctcc agcgccgcag acccggtggt ggccaccgct gtcgagaagc
     2221 cttcctttcg tagcaggaag gcgagcggat ctgcgaagga ttcctcatcc tcgacgatca
     2281 gcaccctggt cacagcgatc ctcccacgtg ctcgggtgtc ggcggcgtgt gcggtatgcg
     2341 ttccgcgctt tgcggatgcg cgggcacccg caaggtgaag gtggatccag tacccggcct
     2401 gctccacagc tgtacgtcac caccgtgatt ggcggcgacg tgcttgacga tcgccaggcc
     2461 taacccggtc ccacccgtgg cccggctgcg tgcctgatcg atccggaaga accgctcgaa
     2521 cacccgttcc tggtgctcag cggcaatccc caggccacga tcggtgacgg cgatctccac
     2581 gaaaccatcc gccagacgcc ggctgaccga caccgggcta cccggcggcg agtaggacac
     2641 cgcgttgtcg accaggttgg acagcgcggt gaccagcaac gtcgcgtcac cgtcgagcaa
     2701 cagcccgctc ggcgggtcga cggtgatcct gatgtcagcc gactccgcgg ccaaccggca
     2761 gcgatccagt gcttcacgca ccacctcatc gacctcgacg gtggccaact cggggagccg
     2821 ctcggcccct tgcagccggg acagcgcgat cagttcggtg accagcgcgc ccaatcgggt
     2881 ggcctcattg aggatcttgg tcccgaaccg tcgcacctcg tgcgggtcgt cggtggcgtc
     2941 caggactgcc tcagccagca gcgccatcgc cccaaccggt gtcttcaact cgtggc
//
LOCUS       DATADN010001086         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_275569, whole genome shotgun sequence.
ACCESSION   DATADN010001086 DATADN010000000
VERSION     DATADN010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275569"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            845..1157
                     /locus_tag="VHH53_13965"
                     /pseudo
     CDS             845..1157
                     /locus_tag="VHH53_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611109.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          273 a          493 c          485 g          256 t
ORIGIN      
        1 gcttaccgag accggttgcc tcaagggctc gattggccat ttccccggcg gggaaccacc
       61 aaccagaggt agcggttttc ccgggtcgcg agttcattga ccgtaagcag tacatacaac
      121 ccggcggacc ccaagaatcg caccctctca aggtccacca ccaccagtga ggcagtggct
      181 agctggcggt cagcaactcg cccagttccg gagcggtcag ggcatcaatc tcgcctccta
      241 cggtgaccga acctgcggac ggtgttcggt cacctgtcga tcgcaggacg gctgtcctgc
      301 ccagggtgct cagccgtcca ccgggcaata actcaagcca ctgcaagggc tgctcgccca
      361 ccccagacag tgcacctcgc cgtctacgac tgtgcggctg gggcgcagcc caatcccggc
      421 agccccggcc atcgaagccc agtgatcggg atggattgtg agcgcgagta ggtcgacgag
      481 tagttcggca tgaggtcggt ggtgcaatcc ggcagcgcgg gccgctgagc catcgaaggc
      541 gcagcatccc tcccgaccgg ggttcgccga tccacatggg cagcgattca ggcccatgta
      601 ttgatcgtcg tggcggccat aggcctatcc cagttgccgg cgagcgtcga gcaagtggtg
      661 gctgacctca tggtggctat gaacggctac ccagcgcaga gaccgctcag cccgacgcgg
      721 gtagttgtat atggcgcttc ggtcccagtc gtcggcactc aggcggccaa ggacgttggc
      781 aaaaagctgc gctgcgacgg tgagtagacg cgccacgtca tcggcttctt gctctgcgta
      841 gccgacggtg tattgcagct tggtccgaca cgggctcatc gagccgggta ggcggcggcg
      901 taaacgccgt gactaccgcc gttgggaacg ccaggcgctg atggcgctgt ggcaaattga
      961 catcgtcggc gggttgttct tcgccgatgg caccgagtgc aaggtcgcgt gaccgggtcg
     1021 atgaccattc ccgattctgt gtgatggccg cggtggtgcg ggccatgggg cgggcggtgt
     1081 gcctggcgtt tgcccaggcg ctacgccgct acggcattcc tgaggaagtg ctcacggaca
     1141 acggcaaaca attcactccc acgccgataa ccaaccgacc gcgcgcacat gaacgcttgg
     1201 tcaccgcttg ggcgacgcag cctacgggcc ggcccctcca gcctccaggg tgctgcgcat
     1261 cgctgacgcg accgcttgcg gcggccttga ccctgatcct ctaccgaccc tgcggcagga
     1321 tcgacaaggg caggccacca cctgcctcac acccaacgcg ccgcccaagc tcaccccgaa
     1381 cccggccgtc cgtggaacac acggcactgt tgcattgagg cgaaggcgtc ctcgccatgc
     1441 accaaagcag cgaggcagag aagacctgtt gcgtggcttc tgggggacag gtgggccagc
     1501 tcggtcg
//
LOCUS       DATADN010001087         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_444866, whole genome shotgun sequence.
ACCESSION   DATADN010001087 DATADN010000000
VERSION     DATADN010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444866"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VHH53_13970"
     CDS             complement(<1..1556)
                     /locus_tag="VHH53_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2291266.1"
                     /translation="MLLSTGRRGGLWTWVLALATLVSAGAALLLPAAIGRALDALPGG
                     GHITWILLACAGLIAIMVSGKVIGDIAVGASNARCTAWLRMTLARHLVAIGPAATRRF
                     EPGDLTTRVTRNANEAGQIGVTAVMIGAGLIPPVGGIVALALIDPWVGLTALAGLLTM
                     AGLLRVFVRDNSETIGDYLHSQGQIAARLLDALAGARTIAAAGSIEWERGRILQPLSG
                     LRTHGARMWHNQVRVASQSAMIGPLLQLVVVGVAGLGVVAGRLTVGELYAASRYAVMA
                     AGIGSAVGFFNKLARARAGGRRAAEVLTEPVTPYGAATLPGTSGAGRLEFRGVRVSIA
                     GEAVINDLNLTVPAGATVAVVGQSGAGKSVLAALAGRLTDPEQGQVLLDGVALPELSR
                     EQLRSGVGFAFERPALVGDTIEEVINFGSRSRSEEKVRRAAVTARVDTFVERLPQGYR
                     TELAAAPLSGGEVQRLGLAREFAHPYRLLILDDATSSLDTVTERQVGAALTTGGGGQT
                     RLIIAHRVATA"
BASE COUNT          277 a          598 c          557 g          248 t
ORIGIN      
        1 gccgtggcaa cccgatgcgc gatgatcagc cgggtctgcc ccccgccgcc ggtggtcagc
       61 gccgccccga cctgccgctc ggtcaccgtg tccaggctcg aggtggcgtc atcgagaatc
      121 agcaaccggt aggggtgcgc gaactcccgg gccagtccga gtcgctgcac ctcgcctccg
      181 gacagggggg ccgcggccag ctcggtccgg tatccctgcg gcagccgctc cacgaaggtg
      241 tccacacgag cggtgacggc ggctcgacga accttctcct cgcttcggct gcgtgaaccg
      301 aagttgatca cctcctcgat cgtgtcaccg accaacgcgg gacgctcgaa ggcgaagccc
      361 accccgctac gcagttgttc ccgggacagc tcgggcagcg ccacgccgtc aaggagcacc
      421 tgtccctgct ctggatcggt gagccgaccg gccagtgcgg cgagcaccga cttgcccgcg
      481 ccggactggc cgaccacggc cacggtggca ccggccggga ctgtgaggtt gagatcgttg
      541 atgaccgctt caccggcgat ggacacccgg acaccgcgga actcgagcct gcccgcgcca
      601 gatgtgcccg gcaatgtcgc ggcgccgtat ggggtcaccg gctcggtgag cacctcagcg
      661 gcgcgccggc cgccggcgcg ggctcgggcg agcttgttga agaatcccac cgccgagccg
      721 atgccggcgg ccatcactgc atagcggctg gcggcgtaga gctcaccgac ggtcaggcgg
      781 ccggcgacca ctcccagccc ggctacaccg acgacgacca gctgcagcaa aggcccgatc
      841 atggcggatt ggctggctac ccgaacctgg ttgtgccaca tccgtgcgcc gtgggtgcgc
      901 agcccgctca gcggctgcag gatccgtcct cgctcccact cgatgctgcc cgcagcggcg
      961 atcgtgcggg caccggccag cgcatcgagc agccgggccg cgatctgtcc ctggctgtgc
     1021 aggtagtccc cgatggtctc ggagttgtcg cggacgaaga cccgcagcag cccggccatc
     1081 gttagcagtc cggccagcgc ggtgagcccc acccacggat cgatcaacgc cagcgcaacg
     1141 atgccaccga ccggagggat caggccggca ccgatcatga ccgcggtcac cccgatctgg
     1201 cctgcctcgt tggcgttgcg ggtcactcgg gtggtgaggt ctccgggttc gaagcggcga
     1261 gtggctgccg gaccgatcgc aaccaggtgc cgggccagcg tcatgcgcag ccaggctgta
     1321 cagcgggcat tactcgctcc gacggcgatg tcaccgatca ccttgccgct taccatgatg
     1381 gcgatcaatc cggcgcaggc gagaagaatc caggtgatat ggccgccacc gggtaatgcg
     1441 tccagagctc ggccgatggc ggctggaagc aggagcgccg cgcctgcgct gaccagagtg
     1501 gccagcgcca gtacccaggt ccacagtccg ccgcgccgac ccgtcgatag cagcaagcgg
     1561 tctgcttgtc tccactttga accgtgcgag ctcgcaggca ccccaatcgg atcgctgttc
     1621 acgagtctcc gcatcgtcgc tatgaccccg acatggagac atatcgagga ttgagacgcc
//
LOCUS       DATADN010001088         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_737621, whole genome shotgun sequence.
ACCESSION   DATADN010001088 DATADN010000000
VERSION     DATADN010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737621"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1379)
                     /locus_tag="VHH53_13975"
     CDS             complement(90..1379)
                     /locus_tag="VHH53_13975"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2291267.1"
                     /translation="MTSPGTHEPTPAWGGNTALSGLSRQNSIYRRTMAVCDELADAAL
                     GGADPVRLTEVFARLVGKRIVLLDSAFRLRAQAGRSGSSRGLRWNRTDPSVDRLLRAL
                     EASRRPLRIPAIPGSLLDEACLVAPVVVRETSLGYLLVLDEQPTADGFEVQPDDAEFL
                     TVTYAATLFALTLAHEQTSAELDLRYRSSVVDALASGHFLDAQDAQRKVRSLGLRDGV
                     PLRVAMLRPAPLDAAAAMQRIAAMLPVAAAAVRDGALVVIVPSDPSTEAFTELCGLEQ
                     GATCGVSERVDSPELVPRAVRQAERAIDLALRLGRAGQPVRYEELGIYRLLCTIGDMQ
                     QLMGFARDVLGPLLDYDAEHRTDMVHTLSVYLHHHGSHKRSARTLHLHANTVAYRAAR
                     IEAITGLDLGDPDDRLVAQVAVKIIESQGLAETGSQG"
     gene            complement(1376..3244)
                     /locus_tag="VHH53_13980"
     CDS             complement(1376..3244)
                     /locus_tag="VHH53_13980"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX2291268.1"
                     /translation="MDAREPRTVLEELCAGEMVICDGAMGTMLHAAGVSLDRSLPELN
                     LSRPSLVRDLHAAYVSAGARILQTNTFGANRLRLAGAGLEDSVSEINIAGARLAREAA
                     AQSGHPVVVAGSVGPAVSAPAVRRVPASAREQTLREQIVALCDWVDLIILETFGDLES
                     LIQAVEVTTSCSDLPVVAQLTFGDDGRTLRGEDPAQAAAVLSSLRVTALGANCTVGPA
                     VLVDVVGELARATDLPVSVQPNAGVPHRHGRQIRYARNAAYFAEAAPRFLAAGATVLG
                     GCCGTTPAHVRAVSHAVAALPPAQRATLRPPVTGATIIRGVPARPEAPCPGWPWEGEL
                     VVAAGLQAPHGPQVPEFIERATRLRAAGAGVLAIIEPAPPAARISPVGAAVVVSERVG
                     SEVLLSVEAADRNLAALQADLLGAYALGLRVIVCRTGSPRVAGDYPSAGGGSPWDVDS
                     VGLVAMLGALNEGIDWRGVATAERTRFVIGAMLRTAPNDLARELDKVEAKLRSGAHFL
                     VTDVIYDVDEATSVLDAVRARGVTLPVLATLAPFEDIRTLQRLSNEVAEASIPLSALA
                     AARRNRHHPEQSVELSVRVVEKLRHLIAGVVVQVPSGMDEPAVQLVEALAQLRSGP"
BASE COUNT          559 a         1224 c         1103 g          489 t
ORIGIN      
        1 accggcgctt gcgactgcaa cggcgctagt gagtgcagcc gctcacatcc cgcattgcgc
       61 attccatcaa cctcgccagc attccttcct caaccctggc tgccggtttc ggctaagcct
      121 tgcgactcga ttatcttgac agctacctgc gcaaccagcc gatcatcggg atcaccgaga
      181 tccagcccgg tgatggcctc gatacgcgca gcgcggtagg cgacggtatt ggcgtgcagg
      241 tgcaacgtgc gggcggaccg cttatgactg ccgtggtgat gcaggtacac cgacagagtg
      301 tgcaccatgt cggtgcgatg ctcggcgtca tagtcgagca aaggcccgag cacatcacgg
      361 gcaaacccca tcaactgctg catgtcgccg atggtgcaca gcaaccggta gatacccagc
      421 tcctcgtagc ggaccggctg gccggcccga cccagccgca gggccaggtc gatcgcccgc
      481 tcggcctggc gcaccgcgcg gggtacaagt tccggggagt ccacccgctc gctcaccccg
      541 caggtggcgc cctgctccag cccgcacaac tccgtgaagg cctcggtact cggatcactg
      601 ggaacgatca cgaccagcgc gccgtcgcgc acggcggcgg ccgcgaccgg taacatcgct
      661 gcgatccgct gcatcgccgc cgccgcgtcc aacggtgccg ggcgcagcat cgcgacgcgt
      721 agtggcaccc catcacgcag tcccagcgac cggaccttgc gctgcgcatc ctgggcgtct
      781 aggaaatggc ctgaggccag ggcgtcaacc acgctgctgc gatagcgcag atccagctct
      841 gcactggtct gctcatgcgc cagtgtcaac gcgaaaagcg tggccgcata ggtgacagtg
      901 aggaactcgg cgtcgtcggg ttgcacctcg aagccgtcgg ccgtgggctg ctcgtcgagc
      961 acgaggaggt aacccaggct ggtttcgcgg accacgacgg gagccaccaa gcaggcctcg
     1021 tcgagcagcg atcccggaat cgcagggatc cgcagtggcc gccgcgaagc ctccagcgcg
     1081 cgcagcaacc ggtcgacgct gggatccgtg cggttccacc gcagccctcg ggatgagccg
     1141 gagcggccgg cctgggcccg cagccggaac gcactgtcga gcaatacgat ccgcttgccg
     1201 accagccggg cgaacacctc agtgagccgc accgggtcgg cccctcccag cgccgcgtcc
     1261 gccagctcgt cacagacggc catggtccgc cggtagatcg aattctgccg ggacagcccg
     1321 ctcagcgcgg tgttgccgcc ccaggccggt gtgggctcgt gcgtccccgg gcttgtcacg
     1381 ggcccgagcg cagctgcgcg agagcttcca ccagctgtac cgccggctcg tccatcccgc
     1441 tcggcacctg cacgacgaca ccggcaatca ggtgtcgcag cttctccacc acgcgcaccg
     1501 agagctcgac gctctgttca gggtgatgcc ggttgcgccg ggcggccgcc agggcagaca
     1561 gcgggatcga tgcttcggca acttcattgg acagcctttg cagggtacgg atgtcttcga
     1621 acggagcgag cgtcgcaagc accggcagcg tcaccccgcg cgcccgcacc gcgtcaagca
     1681 cgctggtcgc ctcgtcgacg tcatagatca cgtcggtcac caggaaatgc gctccggagc
     1741 ggagcttggc ctccaccttg tccagctcgc gggcgagatc attgggtgcg gtgcgcaaca
     1801 tggcgccgat cacgaatctg gtgcgctccg cggtggcaac gcctcgccag tcgatgccct
     1861 cattgagcgc accaagcatg gcgacgagcc cgacggagtc gacgtcccaa ggcgaaccgc
     1921 caccggcgct ggggtagtcg ccggccaccc gcgggctgcc ggttcggcac acgatgaccc
     1981 gcagacccag cgcgtacgca ccgagcaggt cggcttgcag ggccgccagg ttgcggtcgg
     2041 cggcctccac cgacaacagc acctcgctgc ccacccgctc gctgaccacc acggcagcgc
     2101 cgactgggct gatccgggcc gcaggcggcg ccggttcgat gatggccagc acccccgcgc
     2161 cggccgcgcg cagcctggtc gcccgctcga tgaactccgg tacctgcggg ccgtgcggag
     2221 cctgcaaccc ggcggccacc accagctcgc cctcccacgg ccaaccgggg cagggtgcct
     2281 caggtctcgc tggtacgcct cgtatgatgg tcgcgccggt caccggtggg cgcagggtgg
     2341 cgcgctgagc cgggggcaac gcggcaaccg catgggagac cgcccgcacg tgggcaggtg
     2401 tcgtcccgca gcagccaccc agcacggtgg cgcccgcagc caagaaccgt ggcgcggcct
     2461 cagcgaaata cgccgcgtta cgggcatagc ggatctgcct gccatgccgg tggggcacac
     2521 ccgcattggg ctgcacggac accggcaggt cggtggcgcg agccagctca ccgaccacat
     2581 ccaccagcac cgcggggccg accgtgcagt tggcacccaa cgcggtcacc cgcaggctgg
     2641 agagcaccgc ggcagcctgc gcgggatcct caccacgtag cgtccggccg tcgtcaccga
     2701 aggtcagttg ggccactacc ggcaggtcac tacacgacgt ggtgacctcg accgcctgga
     2761 tcaaggactc caggtcgcca aaggtctcta ggatgatcag gtcgacccag tcgcacagcg
     2821 ccacgatctg ctcgcgcagt gtctgctccc gggcgctggc cggtacgcgg cgcaccgcgg
     2881 gggcgctcac cgccggtccg accgacccag cgaccaccac agggtgcccc gactgcgcag
     2941 ctgcctcccg ggccagccgg gcgccggcga tgttgatctc cgagacgctg tcctccagac
     3001 cggccccggc cagccggagc cggttggcac cgaaagtgtt ggtctgcagg atccgggcgc
     3061 ccgcgctgac atacgccgcg tgcaggtcac gaaccaggct cgggcgtgag agattgagct
     3121 ccggcagtga acggtccagc gataccccgg ctgcatgcag catcgtgccc attgcgccat
     3181 cacagatcac catttcaccg gcgcaaagct cttcgagcac cgtccggggc tcccgtgcgt
     3241 ccatcgcctc gcctcctggc ggttggaatt ctccttccgt gactacggag ctattccgtg
     3301 gtgatcgagc aggagcattc cgagttcccg ggctcgcgag gccgcgtgaa cgtcgccttc
     3361 ggccgcggcg acgat
//
LOCUS       DATADN010001089         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1329332, whole genome shotgun sequence.
ACCESSION   DATADN010001089 DATADN010000000
VERSION     DATADN010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329332"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VHH53_13985"
     CDS             complement(<1..698)
                     /locus_tag="VHH53_13985"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEX2291269.1"
                     /translation="MDGAARRLTLQVTSTRALVDCPVCQFPTQRIHSRYVRTVADLPW
                     GPWRVVLHLQVRKFFCANGRCPRRIFTERLAPLVAPWARRTQRLVHWLAHVALALGGR
                     AGVRLSRALGLPISRHTLLRLLRRLPLPAYPTPQVLGVDDWAYKKRQTYGTVLIDLER
                     RRPLALLPDREAKTFALWLQAHPGVGVITRDRSRAYADGARQGAPEAIQVADRLRLRQ
                     NLAEALDQVFHAHG"
     gene            complement(1010..1261)
                     /locus_tag="VHH53_13990"
     CDS             complement(1010..1261)
                     /locus_tag="VHH53_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291270.1"
                     /translation="MRTKRKSHGLAPDLHALEPTLTRYGYNVNAVAELLNIRQAEVRA
                     FLQGQLPPGRVEERHRQLLAAGIPLSDPRALASPAVATG"
     gene            complement(1267..>2039)
                     /locus_tag="VHH53_13995"
     CDS             complement(1267..>2039)
                     /locus_tag="VHH53_13995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2291271.1"
                     /translation="ILHRGPRPLEAFDPDLVDLLVKRTRELGMAVELSTEVRGVERNG
                     GGCTVRATRAGREQRFAADLVVHGAGRIPELDDLDLEAAGVKREARGVTVNQYLQSVS
                     NPAVYAAGDAAASGPPLTPVASHHVDVVTTNLLQGNRVTPNYEGLASAVFTIPPLAAA
                     GLSEAAARQKGLKFRSHWQDTSQWLSTRRLGETTSGAKVLVEEGTDRILGAHLFGPRA
                     DEEINVFAVAIRFGVRAADLKQVFFAYPTVTSDLPYLV"
BASE COUNT          356 a          676 c          694 g          313 t
ORIGIN      
        1 ttgccatggg cgtgaaagac ctggtcgagg gcttcggcga ggttctgccg caggcgcaag
       61 cggtcggcca cctggatcgc ctcaggggca ccttgccggg ccccgtccgc ataggctctg
      121 gaccgatcgc gggtgatcac gccgacaccg ggatgggcct ggagccagag ggcgaaggtc
      181 ttggcctccc gatcaggcaa gagggccagc ggtcgccgcc gttcaaggtc gatcagcacg
      241 gtgccatagg tctggcgttt cttgtaggcc cagtcgtcga cgccgaggac ctggggtgtg
      301 ggataagccg gcaggggcag acgacgcagc agccgcagca gggtgtggcg actgatgggc
      361 aggcccaaag cgcggctcaa tcgcaccccc gccctgcccc ccagcgccaa ggcgacatgc
      421 gccagccagt ggacaagtcg ctgcgtgcgc cgcgcccatg gggcgaccag cggtgcgagc
      481 cgctcggtaa aaatgcggcg ggggcagcgg ccattggcac agaagaactt ccgcacctgc
      541 aggtggagca cgacccgcca tggcccccag ggcagatccg ccaccgtgcg gacatagcga
      601 ctgtggatgc gctgggtcgg aaactggcag acggggcagt caaccagggc tcgtgtcgac
      661 gttacttgca acgtcagccg ccttgctgcg ccgtccatat gccaggcatc caggtgtaga
      721 gggggaacag ccgggaacac acacgacagt accgtcacgt atggccatcg cctccagagg
      781 ggagaaagat cagtttttca ataatagaca ccctggacgg cgatgggaaa aattttcacc
      841 aaaagcgacc cagagccggg attatgtgag aacagcttga ttacagccgt cgcggctaag
      901 attccctgag aataaagtct gggtgggtgc ctgcgatgcc ccgagggccg ctgccgcatc
      961 ttggcgtgcc tcggcgggtc ggtgtgccac cctcacggcg tcgacgacgt cagccggtcg
     1021 ccacggcggg actggcgagc gcgcgtgggt cgctcaaggg aataccggcc gccaagagct
     1081 ggcgatgccg ttcctccacc cgccctggcg gtaactgccc ctggagaaac gcgcgcactt
     1141 ccgcctggcg gatgttcagc aactccgcca ccgcgttgac gttgtagccg tagcgggtca
     1201 gggtaggctc cagggcatgc agatcgggcg ccagcccgtg tgatttccgt ttcgtgcgca
     1261 aagccgtcaa accaggtacg gcaggtccga cgtgacggtc gggtaggcaa agaaaacctg
     1321 cttgagatcg gcggcgcgga cgccgaagcg gatcgcgacc gcgaacacgt tgatctcctc
     1381 gtcggcccgc ggcccgaata gatgcgcacc caggatgcgg tccgtgccct cttccaccag
     1441 taccttcgcg cccgacgtcg tctcgccgag ccggcgggtg ctcagccact gggacgtgtc
     1501 ctgccagtga ctacggaact tgagcccctt ctgtcgagcg gccgcctcgc tcagcccggc
     1561 ggccgcgagc ggcgggatcg tgaacacggc ggaggccagt ccctcgtagt tcggcgtcac
     1621 ccggttgccc tgcaacaggt ttgtcgtgac gacgtcgacg tgatggctgg cgaccggcgt
     1681 gagcggcggc ccgctggcag ccgcatcgcc cgctgcatag acggcggggt tggacacgct
     1741 ctgcagatat tggttgaccg tgaccccacg tgcctcccgc ttcacgccgg ccgcctcgag
     1801 gtcgagatcg tccagctccg ggatgcggcc cgcgccgtgc acgaccaggt cggccgcgaa
     1861 acgctgctcc cggcccgcac gcgtggcccg caccgtgcac cccccaccgt tgcgctccac
     1921 cccgcgcacc tccgtcgaca gctccacggc cataccgagc tcgcgcgtgc gcttgaccaa
     1981 caagtcgacc aggtctggat cgaaggcctc gagggggcgc gggccgcggt gcaggatgg
//
LOCUS       DATADN010001090         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_591816, whole genome shotgun sequence.
ACCESSION   DATADN010001090 DATADN010000000
VERSION     DATADN010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591816"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /gene="ltrA"
                     /locus_tag="VHH53_14000"
     CDS             complement(<1..911)
                     /gene="ltrA"
                     /locus_tag="VHH53_14000"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX2291272.1"
                     /translation="MTTEELGPWLRQHWAAVREALDAGTYRPSPVRRVVIPKPGGGER
                     FLGVPTCLDRLIAQAIAQVLTPIFDPFFSGSSFGFRPGKSAHQAVRVARRCVQDGLEW
                     VVDVDLDRFFDRVGFDVLMARVARKVSDRKVLKLIRRYLEAGVMVEGIRQPTREGTPQ
                     GSPLSPILSNIMLDDLDRELWTRGHRFVRYADDLRVFVGSERAAHRVFDSVCGVIEGR
                     LRLKVNRDKSSIRHAAAATLLGFGFFLHGGQVKIVVAPKAITRLKDQLRGLTRRNWGV
                     SMDYRIGQLNLFTTGWMAYFRLADSART"
     gene            1160..1447
                     /locus_tag="VHH53_14005"
     CDS             1160..1447
                     /locus_tag="VHH53_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2291273.1"
                     /translation="MATRRHFTQEYRDQAVSLVLDSGRSIAEVAKSIGVHEMTLGKWV
                     KKAKDSGRVLDKDLSETERTELERLRKENAILRMERDFAKKVAAWFAKEQP"
     gene            1444..2328
                     /locus_tag="VHH53_14010"
     CDS             1444..2328
                     /locus_tag="VHH53_14010"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX2291274.1"
                     /translation="MKYAAIADWAPDKEYSVAFMCDQLGVVRQGYYRWLADGPCQRER
                     TDTELTDTIREIHRDLHGHPGVRRVWAELVVRGVRVAPKRVWRLMRAAGLRGRHPRAW
                     KRTTVAGQRPVDAPDLIGQDFTAEASDTRWCGDITYVRTVDGWAYTATVIDLHSRKVV
                     GYAVADHLRTSLITEALAAALVIRKPPAGVIFHSDRGCQYTSKEFADFCAVNGVRRSM
                     GRRATCFDNAVSESFFATYKKELIHTRPWNDLTEVRQQTFLWIEGYYNRRRRHSTLNY
                     LTPLEYELGYRKLTDLAA"
BASE COUNT          518 a          888 c          712 g          371 t
ORIGIN      
        1 gtccttgcag agtccgcgag ccggaagtag gccatccagc cggtggtgaa caggttgagc
       61 tggccgatgc ggtagtccat cgacacgccc cagttgcgcc gggtcagacc tcgtagctga
      121 tccttcagcc gcgtgatcgc cttgggagcg accacaatct tgacctgccc gccgtggagg
      181 aagaacccga atcccagcaa cgtcgcggcc gcggcgtgcc ggatcgagga cttgtcccgg
      241 ttcaccttca gccgcagccg cccttcgatc acaccacaga cgctgtcgaa caccctgtgg
      301 gcagcccgtt cgctacccac gaagacccga aggtcatcgg cgtaacgcac gaacctgtga
      361 ccgcgggtcc acagctcccg gtccagatca tccagcatga tgttcgacaa gatcggcgac
      421 agcggagaac cctgcggggt cccctcccgt gtcggttgcc tgatcccctc gaccatcacc
      481 ccggcctcca gatacctccg gatgagcttg agaaccttgc ggtcggacac cttgcgcgcc
      541 acccgcgcca tcagcacatc gaacccaacc cgatcaaaaa accggtccag atcaacgtcg
      601 acgacccact ccaggccatc ctggacacac cgccgcgcga cccgcaccgc ctggtgagcg
      661 gacttgccgg ggcgaaatcc aaatgacgag ccggaaaaga atggatcgaa tatcggcgtg
      721 agtacctgcg caatagcctg ggcaatcagc cgatccaaac aggtggggac ccccagaaaa
      781 cgctcgccgc ccccgggctt ggggatcacc acccggcgca ccggcgaggg ccggtaggta
      841 cctgcgtcca gcgcctcccg aaccgctgcc cagtgctggc gcaaccacgg acccaactcc
      901 tccgtggtca tgccatccac accagacgcg ccccgattag ccctgacccg cctcaacgcc
      961 cggctcagat tccccggtgc aagcatctgc tcccacaacg acatctccaa gcccgggatc
     1021 gcggcctcct cagagtgcgc cgacctgtca ctacgctcca ctgccggact caacggacct
     1081 caccgtctcc acctggaccg tacccgatct tgtagacacc cggtcgtaga ctgccgctga
     1141 ttgcagcgga gggaattgcg tggcgacacg ccgacacttc acccaagaat acagggatca
     1201 ggccgttagc ctcgtcctcg attcgggccg atcgatcgcc gaggtggcga aaagtatcgg
     1261 cgtccacgag atgacgttag gcaagtgggt gaagaaagcg aaagacagcg gccgggttct
     1321 ggataaggac ctgtccgaga ccgagcggac cgagctggaa cggcttcgca aggagaacgc
     1381 tatcctacga atggagcgtg atttcgcgaa aaaagttgcg gcctggttcg cgaaggaaca
     1441 gccgtgaagt atgcggcgat cgcggactgg gcgcccgaca aggagtactc ggtggcgttc
     1501 atgtgcgacc agctcggggt ggtccgccag ggctactacc ggtggctggc cgacgggccg
     1561 tgccagcgcg agcgcaccga caccgagctc accgacacga tccgcgagat ccaccgcgac
     1621 ctgcacggcc accctggcgt gcggcgggtc tgggccgagc tcgtggtgcg cggcgtccgg
     1681 gtcgccccca agcgggtctg gcggctgatg cgcgctgctg gcctgcgcgg acgccacccg
     1741 cgagcctgga agcgcaccac cgtcgcaggc cagcgacccg tcgacgcccc ggacctgatc
     1801 gggcaggact tcaccgccga ggcatccgac acccgctggt gcggcgacat cacctacgtg
     1861 cgcaccgtcg acggctgggc ctacaccgcg accgtgatcg acctgcactc ccgcaaggtg
     1921 gtgggctacg ccgtcgccga ccatctgcgc accagcctga tcaccgaggc cctcgccgcc
     1981 gccctggtga tccgcaagcc tccggccggc gtcatcttcc acagcgaccg cggctgccag
     2041 tacacctcga aagaattcgc cgacttctgt gcggtaaacg gtgtgcgccg ctcgatgggc
     2101 cggcgcgcca cctgcttcga caacgcggtc tccgaatcgt tcttcgcgac ctacaaaaaa
     2161 gaactcatcc acacccgccc atggaacgac ctcaccgagg tccggcaaca aacattcctc
     2221 tggatcgaag gctactacaa ccgccgccgg cgacactcca ccctcaacta cttgacaccc
     2281 ctcgaatacg agctaggata cagaaaatta accgacctgg cagcctaaat cggtgtcaat
     2341 aaaatcggga acactccaaa accacggcgt tccgaccacc ctcaacgcaa tcccaaccag
     2401 ccccagcttg acaactacga ctgatcggag gtctatcggg acacgccacg tcggttcggt
     2461 gacagcaagc accaccccaa gccgaagtc
//
LOCUS       DATADN010001091         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1329676, whole genome shotgun sequence.
ACCESSION   DATADN010001091 DATADN010000000
VERSION     DATADN010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329676"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VHH53_14015"
     CDS             complement(<1..418)
                     /locus_tag="VHH53_14015"
                     /inference="COORDINATES: protein motif:HMM:NF028262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291275.1"
                     /translation="MVMGYSPWLLRILLGGAQRVVRNGIADGPRPHDEAALRQRVADD
                     GVRLLRFLYCDPSGVIRGKQVHAARLAGTVQAGLGLTRAQNAVNVLDELVPVPGLEPV
                     GEIRIVPDVSTYVRLPWLDGVGSVLSDQVGHAGQDWG"
     gene            479..1315
                     /locus_tag="VHH53_14020"
     CDS             479..1315
                     /locus_tag="VHH53_14020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX2291276.1"
                     /translation="MRNLARRIPRWALWTCAALGAVVAVNEMRSKRVPFLRRSFLRLL
                     LAVRRLRADGQVGDAPEEALAAYVLANARQGDVDDVIRVIDDFCYHRSFMMNVGDEKG
                     EILDAAVRRVRPRRLLELGTYCGYSALRMVRVMPSYARLYSIELNPANADIARRIWDH
                     AGVGDRVTVVVGHLGDGGSTLNRLHAEHGFAKRSIDFVFIDHDKAAYLPDLQRILSQS
                     WLHPGSVVVADNVKFPGAPEYRAYMKKQEGRSWYTTEHKTHVEYQSWIKDLVLESEYS
                     GG"
     gene            complement(1336..>2264)
                     /locus_tag="VHH53_14025"
     CDS             complement(1336..>2264)
                     /locus_tag="VHH53_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX2291277.1"
                     /translation="FSSRELPPLLQHGLEEFLARHGHRAVAEVDVGMPRWSDDPTHVL
                     GVLANYLRLADPDLAPDAQFTRGARAAEAAATRMVSAVGRRNWLRAAAVRIALSRVRG
                     LVGMRETHKDYLVRALAHVRQQLTAVGAELANRGLLAKSEDVFFLDLVEARAALEGGD
                     FRAAVAARREDYERELRRRQVPRIMLSDGSLPETLVGPGQAPVEGQLAGTPASAGTVT
                     AIARVVLDPVGAHLEPGEILVAPSTDPGWTPLFLTAGGLVMEMGGANSHGAVVAREYG
                     IPAVVGVPDATLRITTGQRITVHGATGLVELQ"
BASE COUNT          419 a          751 c          729 g          365 t
ORIGIN      
        1 aaccccagtc ctgaccggca tggccaactt ggtctgacag tacgctgccc acaccgtcga
       61 gccacggcag ccgaacatac gtgctgacgt ccggcacgat gcggatctca cctaccggct
      121 ccagcccggg taccggcacc aattcgtcca acacgttgac agcgttctgc gcccgagtca
      181 ggccaagtcc ggcctgcaca gtcccggcca ggcgcgcagc gtgtacctgc ttgccgcgga
      241 tcacgccact gggatcgcag tagaggaacc tcagcaaccg caccccgtca tcggctaccc
      301 gctgccgcag cgccgcctca tcgtgcgggc gcggtccgtc cgcgatcccg ttacggacca
      361 ctcgctgagc acccccaagg agaattcgca acagccacgg tgagtatccc atgaccatga
      421 cccaactcct tgtgatccat ggggaaggat ggagcgacac ctacgcaagg gagtgcgcat
      481 gagaaacctc gcacgacgta tcccacgatg ggcactgtgg acgtgcgcgg cactgggtgc
      541 ggtcgtcgcc gtcaacgaaa tgcgcagtaa gcgggtgccg tttctgcggc gatccttcct
      601 acgtttgcta ctggccgtgc gacgtctgcg cgcggacggg caggtgggtg atgcaccaga
      661 ggaggctctc gccgcctacg tcctggcgaa cgcccggcaa ggcgacgtcg acgatgtcat
      721 cagggtgatt gatgacttct gttaccaccg gagcttcatg atgaacgtgg gggatgaaaa
      781 gggtgagatc ctcgacgcag cggtccggcg ggtccgcccc cggcgtctcc tcgagctggg
      841 tacctactgt ggttatagcg cgttgcggat ggtccgcgtc atgccgagtt acgcccgact
      901 ctactcaatc gagctgaacc cggcgaacgc cgatatcgcc cgccggatct gggatcatgc
      961 aggtgtcggc gatcgggtaa cggtcgtcgt cggccatctc ggcgatggcg gatcaacgct
     1021 gaaccggctg catgcagagc acggcttcgc caagcgcagc atcgactttg tgttcatcga
     1081 ccacgacaaa gcggcctatc tacccgacct tcaacgcatc ctgagccaga gttggttgca
     1141 ccccggatcc gtcgtggtag ccgacaacgt gaagttcccg ggtgcgccgg aatatcgggc
     1201 atatatgaaa aaacaggaag gcaggagttg gtatacgacg gagcacaaga cgcacgtcga
     1261 ataccaatcg tggatcaagg atctggtgct cgagtcagag tattcaggtg gctgaggtca
     1321 ctgaaggatg aatggttact gcaattcgac gaggcccgtc gcaccatgga cagtgatccg
     1381 ctgcccggtg gtgatgcgca gtgtcgcatc ggggaccccg accaccgcgg gtataccgta
     1441 ctcgcgagcg acaacggcac cgtgtgagtt cgccccgccc atctccatga ccagtccgcc
     1501 cgcggtgagg aacagcggcg tccaaccggg atcggtggac ggcgcgacga gaatctcccc
     1561 cggctccagg tgagcgccga ccgggtcgag taccacccgg gcaatcgcgg tcacggtgcc
     1621 ggccgaggcg ggggtgccgg cgagctgtcc ctcgacgggt gcctggcctg ggccgacgag
     1681 cgtctcgggc aagctaccgt cggacagcat gatccgcggt acttgccggc gccgcagctc
     1741 ccgctcgtag tcctcccgac gggcagcgac ggcagctcgg aagtccccac cctccagcgc
     1801 ggcccgggct tcaacaaggt cgaggaagaa cacgtcctcg gacttggcca gcagcccacg
     1861 attggcgagc tcggcaccaa ccgcggtcag ttgctgccgg acgtgcgcca aggcgcgcac
     1921 caggtagtcc ttgtgcgtct cgcgcatgcc gaccaggcct cgtacccggc tcagcgcgat
     1981 gcgcacggcg gcggcccgca gccagttgcg gcgccccact gcgctgacca tccgggtcgc
     2041 tgccgcctcg gctgcgcgag cacctcgagt gaactgcgca tccggggcga ggtccggatc
     2101 ggccagccgc aggtagttgg ccagcacacc gaggacgtga gtcggatcgt cggaccaccg
     2161 cggcatgccc acgtcgacct cggcgacagc ccggtggccg tggcgggcca ggaactcctc
     2221 gagcccgtgt tgcagcaatg gcggcaactc acggctgctg aacc
//
LOCUS       DATADN010001092         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_592191, whole genome shotgun sequence.
ACCESSION   DATADN010001092 DATADN010000000
VERSION     DATADN010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592191"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..345
                     /locus_tag="VHH53_14030"
     CDS             193..345
                     /locus_tag="VHH53_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291278.1"
                     /translation="MDDRTELPQESAAAQAPPPRHTRSLTERLNTWVPMVTAVAALAL
                     SVVTWA"
     gene            370..873
                     /locus_tag="VHH53_14035"
     CDS             370..873
                     /locus_tag="VHH53_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291279.1"
                     /translation="MNLPSIIRIGAESPESFDVYLQPSFSVPRSFDSTARISAVQFAF
                     SLEGAVETRAPNFYWHDSGIFGQLDEGGFGWTYASDPAPFIVGHQEPQRPTIRFIALD
                     ERISPGHWTGTITAARGPGQEALVERFCVVVSEANILDYNAFGIIDFRNDIPNAPTDC
                     YWRPDDW"
     gene            1127..>1665
                     /locus_tag="VHH53_14040"
     CDS             1127..>1665
                     /locus_tag="VHH53_14040"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HEX2291280.1"
                     /translation="MFVPRSGVLPSPVRRTLLYRQATGHLPPLLRPRTFTEKLNWRIT
                     FDRRELLAPSCDKLAMKEHARRLAPGLVRVPETLWAGTDVGELADVDLPDHWVLKPNH
                     SCIRRLFGRGPADVADLRRRTAGWVEERYWRKSEEWAYRRARPCLLVEEFIGVPGVVP
                     ADLKVVVVDGEPRLVELHTG"
BASE COUNT          248 a          587 c          564 g          266 t
ORIGIN      
        1 agctccctga gcgcgtgtca tcagtagcct gtgagggtcg cgggggaggc atctcaccac
       61 ccagctggcg ccacatcggg ggtaccgatg gagaagcagc ctgcaccctc ctgatccgac
      121 ccaccacggg gaagcggaac ctcgcaggag ccccccgtgg ggccgtcagc cggcggctct
      181 gagcggacgt cggtggatga ccgcacggag ctgccacagg agtctgctgc cgcacaggcg
      241 cccccacctc gccacacccg gagcctgacc gagcggctca acacctgggt tccgatggtg
      301 accgcagtgg ccgcactcgc tctctccgtc gtcacctggg cgtagtccca gcggacgccg
      361 gaggtgacca tgaacttgcc gtccatcatc cgcatcggtg ccgagtcccc cgagtccttc
      421 gatgtgtacc tccagccgag cttctcggtc ccgaggagct ttgactcgac cgcgcggatc
      481 tccgccgtac agttcgcatt ctctctcgag ggggcggtgg agactcgtgc accgaatttc
      541 tactggcacg actcggggat cttcgggcag ctcgacgaag ggggattcgg gtggacctat
      601 gcgtctgatc cggcgccgtt catcgtgggc caccaggaac cgcaacgtcc gaccatccgg
      661 ttcatcgcac tggacgagcg catctcaccc ggtcactgga caggcacgat cacggcggcc
      721 cgcggcccag gacaggaagc cctggtggag cgcttctgtg tcgttgtcag cgaggcgaac
      781 atcctggact acaacgcgtt cggcatcatc gacttccgca acgacatccc gaatgctccg
      841 actgactgct attggcggcc agacgactgg tgacgtgccc gggtcctgct cgtcgtcatc
      901 gggtcgcgtt ctcccatggc cgcttggaca gcggctgccc ggtaccgcat cgcagtgacg
      961 ccaccgggac aggcccgcgc cgtgactcat ggagcggcac cgttgtgccg accggtcacg
     1021 gcaccgggcc ccgaccacag tggtcgggcg ctgtgcacag gtccggcggg acttgcgaga
     1081 cctgcgcgcg gttgccggtc gccgtcccgg ctgttggggt tggtaggtgt tcgtcccgcg
     1141 ctccggcgtc ctgccgtcgc cggtgcgccg gaccctgctc taccgacagg ccaccgggca
     1201 cctcccgccg ctcctgcggc cacggacctt caccgagaag ctcaactggc ggatcacctt
     1261 cgaccggcgg gaactgctcg cacccagctg cgacaagctg gcgatgaagg agcacgcccg
     1321 ccggttggcg ccgggcctgg tgcgcgtccc ggagaccctc tgggccggca ccgacgtcgg
     1381 cgagctggcg gacgtcgacc tgcccgacca ctgggtgctc aagcccaacc actcctgcat
     1441 ccggcggctg ttcggccgcg ggcccgccga cgtcgccgac ctgcgccgga ggacggccgg
     1501 ctgggtggag gagcggtact ggcgcaagag cgaggagtgg gcctaccggc gagcgcggcc
     1561 gtgcctgctc gtcgaggagt tcatcggcgt cccgggcgtg gtaccggctg acctcaaggt
     1621 ggtggtcgtc gacggcgagc cccggctggt cgagctgcac accgg
//
LOCUS       DATADN010001093         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1330143, whole genome shotgun sequence.
ACCESSION   DATADN010001093 DATADN010000000
VERSION     DATADN010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330143"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VHH53_14045"
     CDS             complement(<1..290)
                     /locus_tag="VHH53_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291281.1"
                     /translation="MLISGPRRGFGAGRTLGLAAIATAVGMTVVSGCGSPGGDQPGAA
                     TPQSIVTSTTKIAGAGVLGNQRKPDESCAPDPMPVDPGPPDRMVRHAAGETRV"
     gene            440..>1881
                     /locus_tag="VHH53_14050"
     CDS             440..>1881
                     /locus_tag="VHH53_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2291282.1"
                     /translation="MEDLLVAEAPPIGELEARRPFPARLGPKGNLVYKLITTTDHKMI
                     GIMYCVACFIFFFIGGLMALFMRTELAMPGLQFLSNEQFNQLFTMHGTVMLLFYATPI
                     VFGFATPDGAASFGWTAYQPLAGPQHSPGVSGNMWYVGLVLSGLGTILGGVNMITTIV
                     TLRAPGMTMFRMPIFTWNILVTSILVLLAFPLLTAALFGLAADRHLGAHIYDPANGGV
                     LLWQHLFWFFGHPEVYIIALPFFGIVTEVIPVFSRKPIFGYTTLIYATIAIAALSVAV
                     WAHHMYATGAVLLPFFSFMTFLIAVPTGIKFFNWIGTMWKGQLTFETPMLFSVGFLLT
                     FLLGGLSGVLLASPPLDFHVTDSYFVIAHFHYVLFGTIVLATYAGIYFWFPKMTGRLL
                     DERLGKLHFWLTFIGFHTTFLVQHWVGDEGMPRRYADYLPSDGFTTLNIVSTIGAFVL
                     GVSVLPFVWNVIKSSRYGEPVTVDDPWGYG"
BASE COUNT          287 a          638 c          576 g          380 t
ORIGIN      
        1 acccgggtct cgcccgccgc gtgacgcacc atgcggtccg gcggccccgg atccacgggc
       61 atggggtcgg gtgcgcacga ctcgtcaggc ttgcgctggt tgcccagcac gccggccccg
      121 gcgattttcg tcgtactggt cacgatcgac tgtggtgtgg cggcgccggg ctggtctccg
      181 ccgggggagc cgcaaccgct gaccacagtc attccgactg ccgtggcgat cgccgccagc
      241 cccagcgtcc ggcccgcccc gaaacctctt cgcggtccgc tgatcagcac gtcgccgacg
      301 gtaacatccg accagctcca gggcggtgag ccgacgtggg tgagcgaata cgacggtatg
      361 taggaccgcc cccgacttgc cggtgagacg gcagctagga ttcatcgaga aaaccgcggg
      421 gatgtcccga cctggatgta tggaggacct gttggtagcc gaagcgcccc caatcggaga
      481 actcgaggca cggcgtccgt tcccggcgcg actcggcccc aagggcaacc tggtctacaa
      541 gctcatcacc accaccgatc acaagatgat cggcatcatg tactgcgtcg cctgcttcat
      601 cttcttcttc atcggcgggc tgatggcgct gttcatgcgc accgagctcg ctatgccggg
      661 gctgcagttc ctgtccaacg agcagttcaa ccagctgttc accatgcacg gcacggtgat
      721 gctgctgttc tacgcgaccc cgatcgtgtt cgggttcgcc actcccgacg gggcggcgag
      781 cttcggctgg accgcttacc agcccctcgc aggcccgcag cacagcccag gggtgagtgg
      841 caacatgtgg tatgtcgggc tggtgctgtc gggtctgggc acgattctcg gcggcgtcaa
      901 tatgatcacc acgatcgtca cgctacgggc tcccggcatg acgatgttcc ggatgccgat
      961 cttcacctgg aacatcctgg tgacatcgat cctggtgctg ctggccttcc cgctgctgac
     1021 cgcggcgctg ttcggactgg ccgccgaccg ccacctcggc gcccacatct acgacccggc
     1081 caacggcggt gtcctgttgt ggcagcacct gttctggttc ttcggccacc ccgaggtcta
     1141 catcatcgcg ctgccgttct tcggcatcgt caccgaggtc atcccggtgt tctcccgcaa
     1201 gccgatcttc ggctacacca cgctgatcta cgcgaccatc gcgatcgccg cgctgtcggt
     1261 cgcggtgtgg gcgcaccaca tgtacgccac cggtgcggtg ctgctgccgt tcttctcgtt
     1321 catgacgttc ctcatcgcgg tgccgaccgg tatcaagttc ttcaactgga tcggcacgat
     1381 gtggaaaggc cagttgacgt tcgagacacc gatgctgttc tcggtgggct tcctgctgac
     1441 gttcctgctc ggcggtctgt cgggtgtgct gctggccagc ccgccgctgg acttccacgt
     1501 gacagacagc tacttcgtca tcgcgcactt ccactacgtg ctgttcggca ccatcgtgtt
     1561 ggcgacgtac gccggcatct acttctggtt cccgaagatg accggccgcc tgctcgacga
     1621 gcggctcggc aagctgcact tctggctgac gttcatcggc ttccacacca ccttcctggt
     1681 gcagcactgg gtcggtgacg agggcatgcc gcgccgctac gccgactacc tgccctcgga
     1741 cgggttcacc acgctgaaca tcgtgtccac gatcggcgcc ttcgtcctgg gtgtgtcggt
     1801 cctgccgttc gtgtggaacg taatcaagag ctcacgctac ggcgaaccag tgacggtcga
     1861 cgacccgtgg ggttatggca a
//
LOCUS       DATADN010001094         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_120363, whole genome shotgun sequence.
ACCESSION   DATADN010001094 DATADN010000000
VERSION     DATADN010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120363"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1483)
                     /locus_tag="VHH53_14055"
     CDS             complement(320..1483)
                     /locus_tag="VHH53_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2291283.1"
                     /translation="MAERTGCVGVGGGPAGMVAGLLLARAGVEVTVLEKHPDFLRDFR
                     GDTVHPSTLTLLDELGLGEKFDAVPHSELTAAGFPTDDGAMMTLADMTRLKVPHPYIA
                     MVPQWDFLDLLADSAAKEKTFTLRMATEMTGLIRQGGRVTGISYQTAEGTEGELRADL
                     VIAADGRWSLARREAGLQPKEYDCPFDVWWFRLSRHDDEGDGMLTPRMRNRRFAVPLP
                     RTGYFQIAYLAPKGADLRKEGIEAFRANVTEVCPEFADRVHELETMDDVKFLDVRLNL
                     LHQWHIDGLLCIGDAAHAMSPVGGVGINLAVQDAVAAATLLAQPLRRGRPTLADLEKI
                     RSRRLLPTMLVQGLQRLLHHSVIRPVMAGKRGWSDPGDGVHRGAVPAQCTGPA"
BASE COUNT          262 a          526 c          511 g          255 t
ORIGIN      
        1 accggaacac ccagccgcgc cagaccgagc gcgacgttag ccgggctacc gcccggatgg
       61 gcgacgaacg tccgacctcc gcgctggccg accaggtcga caagcgcctc cccgacgaca
      121 acaaacatgc acatcaccac ccggaccgag cgcggcgacg caggcacaac tcgcgctcgg
      181 cgagctggcg ggtaggatcg catagctgac ggcgtgtctc atactcacgc tggctatcac
      241 gcccgcgcgt atgcgagtcg cccatgccca gtacataccc gctaaaccgc gcaggcgaca
      301 ccaccagtcg tgtcccggat cacgcgggcc cggtgcactg agcaggaaca gccccgcggt
      361 gaacaccatc gcccgggtcc gaccaaccgc gcttgcccgc catcaccggg cgtatcacgg
      421 aatggtgcag tagccgctgc aggccttgca ccagcatcgt gggcagcagg cggcgcgacc
      481 ggatcttctc caggtcggcc agggtcggcc ggccacggcg aagcggctgg gccagcagcg
      541 tcgcagcggc gacggcgtct tgcacagcca ggttgatacc taccccgccg accggtgaca
      601 tggcgtgtgc ggcgtcgccg atgcacagca ggccgtcgat gtgccattgg tgcagcaggt
      661 tcagtcgcac gtcgaggaat ttgacgtcgt ccatcgtttc cagttcgtgc acccggtcgg
      721 cgaattcggg acagacttcg gtgacgttgg cgcggaaggc ctcgatgcct tccttgcgca
      781 ggtcggcgcc cttgggggcg aggtaggcga tctggaagta accggtgcgc ggcagcggca
      841 cagcgaagcg gcggttgcgc atccgtgggg tgagcatgcc gtcgccttcg tcgtcgtggc
      901 gcgaaagccg gaaccaccag acgtcgaacg ggcagtcgta ctccttgggc tgcaggcccg
      961 cctcgcgccg ggccagcgac cagcggccgt cggcggcaat gaccaggtcg gcgcgcaact
     1021 caccttcggt gccctcggcc gtctggtagg agatgccggt gacccggccg ccttggcgga
     1081 tcaagccggt catctcagta gccatccgca gcgtgaaggt cttttctttg gcggcactgt
     1141 cggctagcag gtcaagaaag tcccactgcg gcaccatcgc gatgtagggg tgtggcacct
     1201 tcagccgggt catgtcggcc agcgtcatca tggcgccgtc gtcggtgggg aacccggcgg
     1261 cggtgagctc gctatgtggc acggcgtcga acttctcgcc cagtcccagt tcgtcgagca
     1321 gggtcagtgt tgaggggtgc accgtgtcgc cgcggaagtc gcgcaggaag tcggggtgct
     1381 tttctaacac cgtcacctcg accccggccc tggccagcag cagccctgcc accatgccgg
     1441 ccggaccacc cccgacgccc acgcaacccg tgcgctcggc catctcgata tcctcttttt
     1501 catcatttgt tgaatatgtt tcaggatgcg cctgaactgg ttggtgcgtc aagc
//
LOCUS       DATADN010001095         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1498979, whole genome shotgun sequence.
ACCESSION   DATADN010001095 DATADN010000000
VERSION     DATADN010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498979"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /gene="frr"
                     /locus_tag="VHH53_14060"
     CDS             <1..509
                     /gene="frr"
                     /locus_tag="VHH53_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016880161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX2291284.1"
                     /translation="SVAKEDLATVRTGRASPAMFSKIVVEYYGAATPLIQLASVTVPE
                     ARMAVIKPYDPSLLRPMAKAIRDSDLGVNPTDEGTIIRVIIPQLSQERRRDLVKVAHA
                     KGEDAKVSIRNIRRKAKDELSRISRQGEAGEDEVTRAEKELDATTHRYVGQVDELVKH
                     KESELLEV"
     gene            548..1387
                     /locus_tag="VHH53_14065"
     CDS             548..1387
                     /locus_tag="VHH53_14065"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX2291285.1"
                     /translation="MHPDQSPRATRAGRNLWAAITVGLLLGGGILVALVTVRQVFIGI
                     IAVAVAVATWELAAALRHAGTRIALPPVLFGGQAMVWLSWPFGRDGLLVAFMITALVC
                     LVWHFPQGAGGYVRDVSASVLTAVYIPGFAAFATLLVVPADGAARVLCLMLAAVASDI
                     GGYAAGVLRGRHPMAPSISPKKSWEGFIGSLVAGTAAGALTLTYVFDGQWWQGLLFGA
                     AIVITATGGDLIESLIKRDLGVKDMGSLLPGHGGLMDRLDSLLPSAVVAWLLLSLFVP
                     AGG"
     gene            complement(1404..>2191)
                     /locus_tag="VHH53_14070"
     CDS             complement(1404..>2191)
                     /locus_tag="VHH53_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2291286.1"
                     /translation="STTDPARSRDFYAGLFGWTYHIDPDPNRGYYTNALRDGLPVAGL
                     GGVPVPAGQPVTWTLYLATANIAHTAEVFDQWGGQVLYGPVDVPGQGSMLIGADPTGG
                     VIGFWQPARPWMFHTADTGALYWAELNTRDGRRADEFFANLFGYHQQQIGDGVDMDYT
                     TWSRGGQMLLGRIQMGENWSPDITAHWMLHFVVDPHTGTDAAVNRVAELGGHVHIYPY
                     DSELGRIALVSDPSGAAFALVDPTVTLEPATNSTGTADDPYDD"
BASE COUNT          335 a          690 c          766 g          400 t
ORIGIN      
        1 tgtcggtcgc caaggaagac ctggccacgg tgcgtactgg ccgagccagt ccggcgatgt
       61 tctccaaaat cgtcgtggag tactacggcg ccgctactcc attgatccag ctggccagtg
      121 tgacggtccc ggaggccaga atggctgtga tcaagccgta cgaccccagc ctgctgcggc
      181 ccatggcgaa ggcgatccgg gattcagacc tcggcgtcaa ccccaccgac gagggcacga
      241 tcatccgcgt gatcatcccg cagctttccc aggaacgccg ccgggacctg gtcaaggtcg
      301 cgcacgccaa gggggaggac gccaaggtgt ccatccgcaa catccggcgc aaggccaagg
      361 acgagctcag ccgcatctct cgtcaggggg aggctggtga ggacgaggtg acccgggctg
      421 agaaagagct tgatgccacc acccaccggt acgtgggaca ggtcgacgag ctcgtcaagc
      481 acaaggaatc ggagctgctc gaagtctgag tgcgtggcac cggactcctc gagcacccgc
      541 cgccgctgtg cacccagacc agagcccccg cgccacgcgg gcagggcgca acctgtgggc
      601 ggcgatcacc gtcgggttgc tgctgggtgg cgggatcctg gtcgcattgg tcaccgtgcg
      661 ccaggtattc atcgggatca ttgcggtggc ggtggcggtg gcgacctggg agctcgccgc
      721 ggcgttgcgc cacgccggta cccggatcgc attgccaccg gtgttgttcg gcgggcaggc
      781 catggtgtgg ttgtcctggc cgttcggtcg ggacggttta ctggtcgcgt tcatgatcac
      841 agcgttggtc tgcctggtgt ggcactttcc tcagggtgcg ggcggttatg tccgcgacgt
      901 cagcgcttcg gtgctcaccg cggtctacat acccggattc gccgcctttg ccacgttgct
      961 ggtcgtccct gccgacgggg ccgcccgggt gctgtgcctg atgctcgccg cggtggcttc
     1021 ggacattggg ggttacgccg ccggcgtgtt gcgtgggcgg catccgatgg cgccgtcgat
     1081 aagcccgaag aagtcctggg aaggcttcat cggatctctg gtggccggga ccgctgccgg
     1141 tgccctgacg ctgacctacg tgtttgacgg gcagtggtgg caagggctgc tgttcggggc
     1201 ggcgatcgtc atcacggcca ccggagggga tctgatcgaa tcgctgatca agcgtgacct
     1261 cggggtcaag gacatgggta gcctgctgcc cggtcatggc gggctcatgg accggctcga
     1321 ctcgctgctg ccgtcagccg tcgtggcctg gctgctgctg agcctgttcg tcccggccgg
     1381 tggctgaggc tggacgggct cgctcagtcg tcgtatgggt cgtcggccgt cccggtactg
     1441 ttcgtcgccg gctcgagcgt gacggtgggg tcaaccaacg cgaaggcggc accggaggga
     1501 tcggagacca gcgcgatccg gcccaactcg ctgtcgtaag ggtagatgtg cacgtgaccg
     1561 cctagttcgg cgacacggtt gaccgcagcg tcggtgccgg tgtgcgggtc caccacgaag
     1621 tgcagcatcc agtgcgcggt gatgtcggga gaccagttct cgcccatctg tattcttccc
     1681 agcagcatct gcccgccacg tgaccaggtg gtgtagtcca tgtctacgcc atcaccaatc
     1741 tgctgctggt ggtagccgaa caagttcgcg aagaactcgt cggcccgcct accgtcccgg
     1801 gtgttcaact cggcccagta cagcgccccg gtgtcggcag tgtggaacat ccatggcctt
     1861 gcaggctgcc agaagccgat cacaccgccg gtcgggtcgg ccccgatgag catgctgccc
     1921 tgccctggga cgtccaccgg cccgtagagg acctggcctc cccactggtc gaacacctcg
     1981 gcggtgtgag cgatgttggc ggtggcgaga tacaacgtcc aggtcaccgg ttgccctgcc
     2041 ggcactggga ctccccccag accggccacc ggcaggccgt cgcgcagggc gttggtgtag
     2101 tacccgcggt tggggtctgg gtcgatgtgg taggtccagc cgaaaagtcc ggcgtagaag
     2161 tcccggctgc gggccggatc ggtcgtcgac a
//
LOCUS       DATADN010001096         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1048683, whole genome shotgun sequence.
ACCESSION   DATADN010001096 DATADN010000000
VERSION     DATADN010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048683"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VHH53_14075"
     CDS             <1..300
                     /locus_tag="VHH53_14075"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX2291287.1"
                     /translation="PPAETREMIKTGGFDAVCFTSSSTVRNLVGIAGKPHARTIVACI
                     GPATAETAVEFGLRVDVQPEVAQVDALVDALAEHTAQLRAEGALPPPRKAKSRRR"
     gene            399..1193
                     /gene="hemB"
                     /locus_tag="VHH53_14080"
                     /pseudo
     CDS             399..1193
                     /gene="hemB"
                     /locus_tag="VHH53_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
     gene            complement(1191..1526)
                     /locus_tag="VHH53_14085"
                     /pseudo
     CDS             complement(1191..1526)
                     /locus_tag="VHH53_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
BASE COUNT          244 a          523 c          533 g          226 t
ORIGIN      
        1 ccgcccgccg agacccgtga gatgatcaag accggtggct tcgacgcggt gtgcttcacg
       61 tcctcatcca cggtgcgcaa tctggtcggt atcgcgggca aaccgcatgc tcgcacgatc
      121 gtggcgtgca tcggtccggc caccgcggag actgcagtcg agttcggtct ccgggtggac
      181 gtgcagcccg aggtggccca ggtcgacgcc ctggttgacg ccctggctga acacaccgcc
      241 cagctgcgcg ccgaaggcgc cctgccccca ccccgcaaag ccaagagccg ccgccgctag
      301 ccccgtcccc ttcccgcgtt ctggggacag actgcagtag ttcgggcctc ggccaccgcg
      361 ttctggggac agactgcact agtcgccgga ggaacaggat gtacccaacg agcaggccgc
      421 ggcgattgcg ggctacggcg gctatgcggc gattggtaag ggaaacctcg gtggaggcgc
      481 accagttggt gttgccggtg ttcgtcaagc agggtgccac cgaaccgctg ccgatcgcag
      541 ccatgccagg cgtcgtccag cacagtccgg aatcgctgcg caaagccgcc gctgatgcag
      601 tggcagccgg ggtgggcggg atcatgctgt tcggcgtgcc cactaccagg gatgccaccg
      661 gctccggcgc caccgacccc gatggcgtgc tcaacgcggc actacgcact gtgcgggccg
      721 aagtcggcga cgacatcgtg atcatggcag atacctgctt ggacgagttc accgatcacg
      781 ggcattgcgg cctgcttgat gccaccggcg gggtggacaa cgacgccacc ctcgcggtgt
      841 acgccgagat ggccgtcgct caggtccgag cgggcgccca cctggtaggg ctcagcggca
      901 tgatggacgg ccaagtcgga gtggtccggg aggcactgga cgccgccggg caccatgacg
      961 cgggaatcct ggcctacagc gccaagtacg cctcggcgct ctacgggccg ttccgcgatg
     1021 cggtggaatc acagttgacc ggcgaccgca tgacctacca gcaggacccc gcgaacgccc
     1081 gggaggcgct gcgggaggcg gcgttggacc ttgccgaggg tgcggacatc gtgatggtca
     1141 agcccgcgat gtcctacctg gacgtgctgc aggccgtcgc caatgtctcc gaccgggacg
     1201 tcggagacct cggcgacggc ctgcaacaca tccaggtagc acatcgcggg cttgaccatc
     1261 acaatgtccg caccctcggc aaggtccagc gccgcctccc gcagcgcctc ccgggcgttg
     1321 gccgggtcct gctggtagcc cttccggtcg ccggcccggc cgtcggccgc gacgatctgc
     1381 acgtcgacgg cgtcgcggaa ggggccgtag agcgccgagg cgtacttggc ggcgtaggcg
     1441 aggatcggca cggtggcgtg gcccgcggca tcgagggcgg cgcggatggc cgccacctgc
     1501 ccgtccatca tcccgctggg agcgac
//
LOCUS       DATADN010001097         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_446223, whole genome shotgun sequence.
ACCESSION   DATADN010001097 DATADN010000000
VERSION     DATADN010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446223"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1939
                     /locus_tag="VHH53_14090"
     CDS             <1..1939
                     /locus_tag="VHH53_14090"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX2291288.1"
                     /translation="ARLREGGVVPDTPAKSATWSWFSGSTEGGDTAEIPQTAETVDFE
                     AVDSDPRNADTGNAEAGNLAAEPDPTPAQREGTPPLPPWDAQPSTRGRPLRWGGIAAA
                     VVLGLLVLIYGIDLAMSRGDVPRGVIVAGVDVGGMQQAAAEQRLREQLEPRLNHPVTL
                     RAGDVETTLDPVRAGLTLDWPGTLAQAGAQSLNPWTRLASTWRTHEVAVVTKGNQEAL
                     TAALEGLRAQIDREPVEGTIRFEGARPIPVDPKPGQRLDVPAASNVVLGIWVHGGTVV
                     VPVATLPVSTTSDGIQEALRDVVEPAVSGPVNVTGDGKDAVLKPEVTATALRFAPDGE
                     GGLTTTIDEPTVIAALEPQLASTLRPGKDAEIILQNGLPVVLPSLDGRGIDWSASLRP
                     LFDVLQVSGRERTMAASYVSLPPKLSTEQANALGITTQISTFTTGGFAPDSGQNIRRV
                     AEEVNGAIVRPGETFSLNKYTGPRNAAAGYVEAGIIDHGRPGRGIGGGISQFATTIYN
                     AAYFAGMTDIEHKEHSYYISRYPAAREATVFEGAIDVKFRDDSPTGVLIQTAWTPTSI
                     TVTFWGTKHVDVESITGPRTDVTEPTTQTVYGQPCKNSKGSQGFTVTDTRVVRDANTK
                     AEISRKTRKVVYEPAPHVVCA"
     gene            complement(1968..>2786)
                     /locus_tag="VHH53_14095"
     CDS             complement(1968..>2786)
                     /locus_tag="VHH53_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX2291289.1"
                     /translation="APRVTITHHPDVRRMLMLQKAYAEGLRALYLYTATYQDTVELGG
                     EDAERAARVNDLLLPVVKGVGSERAYEMLTLSLQTLGGSGFLQDYPIEQYIRDAKIDS
                     LYEGTTAIQSQDFFFRKIVRDRGQAIGHLNSEMQRFLDAAGDSSDTGRLKEERALLAT
                     ALGDLQAMLSAMIGFVMSAQQDPRNTYKVAQQTVRFLMSTGDVLIGWLLLRQAEVALA
                     ALDGEVTAKDQAFYQGKVAAANFFARNILPELTARRAIVEATDNALMDVPEAAF"
BASE COUNT          498 a          923 c          922 g          443 t
ORIGIN      
        1 ggctcgtcta cgggaaggag gtgtggtgcc tgatacgcca gcaaagtccg cgacatggtc
       61 gtggttcagc gggtcaaccg aaggcggcga caccgccgag atcccgcaga ccgctgagac
      121 agtggacttc gaggcagtgg actctgaccc aaggaacgct gacacgggga acgctgaggc
      181 aggaaacctc gctgccgagc cggaccccac accagcacaa agagaaggga caccaccgct
      241 cccaccgtgg gatgctcagc cgagcacccg cgggcggcct ctgcgctggg gtgggatcgc
      301 cgcggctgtc gtgctcggcc tgctcgtgct catctacggg atcgatctgg cgatgtcccg
      361 gggtgacgtg ccacgcggcg tcatcgtcgc gggtgtcgat gtgggtggga tgcagcaggc
      421 cgcagccgag cagcggctgc gtgagcagtt ggagccacgg ctgaaccacc cggtcacgct
      481 gcgcgcgggt gacgtcgaga ccaccctgga cccggtacgc gctggtctca ccctggactg
      541 gcctggcacg ctcgctcagg cgggtgcaca gtccctcaac ccgtggaccc gccttgcctc
      601 gacctggcgg acccacgagg tcgccgtggt gaccaagggt aatcaggaag ccctgaccgc
      661 ggcactggaa gggttgcggg cacagatcga ccgggagccg gtcgagggca ccatccgctt
      721 cgaaggcgcg cggccgatcc cggtcgaccc caaacctggg cagcggctgg atgtgcctgc
      781 tgccagcaac gtggtgctgg gcatctgggt ccacggtggc acggttgtgg ttccggtcgc
      841 gaccctgccg gtgagcacta cctccgacgg aatccaggaa gcgctgcgcg acgtcgtcga
      901 gcctgcggtg tccggacccg tcaacgtcac cggtgacggc aaggacgccg tgctcaaacc
      961 ggaagtgacc gcgacggcgc tgcgattcgc cccggacggc gagggtggct tgaccaccac
     1021 catcgacgaa cccacagtga ttgcggcact tgagcctcag ctcgcgtcga cgctgcgacc
     1081 gggcaaggac gccgagatca ttctccagaa cggcttgccg gtggtcctgc cttctctcga
     1141 cggccgcggt atcgactggt cggccagctt gcgtccactg ttcgacgtgc tgcaggtctc
     1201 cgggcgtgaa cggaccatgg cggccagcta cgtcagtctg cccccgaagc tgagcaccga
     1261 gcaggccaat gcattgggga ttaccaccca gatcagcacc ttcaccaccg gcgggttcgc
     1321 cccggattcc gggcagaaca tccgccgggt cgccgaggag gtcaacggcg ccatagtgcg
     1381 gccgggggag acgttcagcc tcaacaagta caccggtccg cgtaatgcag ccgcgggata
     1441 cgtcgaggcc ggcatcatcg accacggccg tcctgggcgg ggtatcggcg gtggcatttc
     1501 ccagttcgcc accacgatct acaacgcggc gtacttcgcg ggcatgaccg atatcgagca
     1561 caaggagcac agctactaca tcagccgcta ccccgcggcc cgcgaggcga ctgtgttcga
     1621 gggcgctatc gacgtcaagt tccgcgacga ctcacccacg ggagtcctca tccaaaccgc
     1681 ctggaccccg acctcgatca ccgtgacgtt ctggggcacc aaacacgttg atgtcgagtc
     1741 gatcaccgga ccgcgcaccg acgtcaccga gccgacaact cagaccgtct acggccagcc
     1801 gtgcaagaac agcaagggct cgcaaggttt cacggtgacc gatacccggg tggtccgcga
     1861 cgccaacacc aaggccgaga tcagccgcaa aacccgcaag gtcgtctacg aacccgcccc
     1921 ccacgtcgtc tgcgcctgat ctccatcgct gaatgcgccg aagaaagtta gaaggcggct
     1981 tcggggacgt ccatgagggc gttgtcggtg gcctcgacga tggctcggcg ggcggtgagt
     2041 tcgggaagga tgttgcgggc gaagaagttc gccgccgcga ccttgccctg gtagaaggcc
     2101 tgatccttgg cggtcacctc gccgtccagg gcggccaggg ccacttcagc ctggcgcagc
     2161 agtagccagc cgatcaggac gtcgccggtg ctcatcaaga accgcacggt ctgctgggcg
     2221 accttgtagg tgttgcgcgg atcctgctgg gccgacatca caaagccgat catggcgctc
     2281 agcatggcct gcaggtcacc cagcgcggtg gccagtaacg cccgttcctc tttgagccgg
     2341 ccagtatcgc tactgtcgcc cgcggcatcc aggaacctct gcatctcgct gttcagatgc
     2401 ccgatcgcct gaccccgatc ccggacgatc ttgcggaaga agaagtcctg ggactggatg
     2461 gctgtggtgc cctcgtacag gctgtcgatc ttggcgtccc ggatgtactg ctcgatgggg
     2521 tagtcctgca ggaacccgga cccacccagg gtctgcagtg agagcgtgag catctcatag
     2581 gcccgctcgg agccgacacc cttgacgaca ggcagcagca ggtcattgac ccgggcggcc
     2641 cgctcggcgt cctcgccacc gagttccacc gtgtcctgat acgtcgcggt gtagaggtac
     2701 agcgcccgca ggccctcggc gtaggccttc tgcagcatga gcatccggcg gacgtcgggg
     2761 tggtgggtga tggtgacccg cggcgc
//
LOCUS       DATADN010001098         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_878760, whole genome shotgun sequence.
ACCESSION   DATADN010001098 DATADN010000000
VERSION     DATADN010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878760"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VHH53_14100"
     CDS             complement(<1..231)
                     /locus_tag="VHH53_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX2291290.1"
                     /translation="MRRAAIYCRISDDRKGAGLGVARQEADCRERAARLGWTVAGLYI
                     DNDLSAYSGRVRPEYRRMLDDLRSDVVDAVIAW"
     gene            complement(262..1155)
                     /locus_tag="VHH53_14105"
                     /pseudo
     CDS             complement(262..1155)
                     /locus_tag="VHH53_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1155..1535)
                     /locus_tag="VHH53_14110"
     CDS             complement(1155..1535)
                     /locus_tag="VHH53_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX2291291.1"
                     /translation="MGTKTQAADRHSDGLGKRGEDLAADYLAGAGLFVLSRNWRCRDG
                     ELDLVATDGERLIVCEVKTRSGTGYGEPSEGVTPAKAARIRRVAATWLRTYRVGWCEV
                     RFDVLAILCPQDGPVTVEHLQGAF"
BASE COUNT          298 a          649 c          547 g          271 t
ORIGIN      
        1 ccaagcgatc accgcatcca caacatccga tcggagatca tcaagcatcc gccggtactc
       61 cggccgaact ctgcctgaat aggccgacag gtcgttgtct atgtacagcc cggcgaccgt
      121 ccaacccagc cttgctgccc gctcccggca gtccgcctcc tgccgagcca cacccaagcc
      181 agcgcctttc cggtcgtcac tgatgcggca gtagatggca gctcgacgca tgagcctcag
      241 agtacattaa tatatcctcc cgtgtgcccg gctcaccgca ccgggcttgg ccagtcccga
      301 accgccgccg atcagcgcgg ccagcgagct ggtgtggtgc ggcgcgacaa acgggggtcc
      361 ggtgatcagc ggggtctcgg tagacagcag gccggccacc gagtgcaccg cggtgacctc
      421 gagtgcctcc tggtcagtca gcgctggcag caaccccacc atccgctggg ccaacatcgt
      481 tttgcccgtg cccggtggcc cggtcagcag caggtggtga ccccctgcgg cggcgacctc
      541 caccgcccag cgagcgtcgc cttggtgcag cacgtcagca agatcgggga ccgccaccgg
      601 tggggaggca atcagcggtc cgggatgggt cagccccacg gcgggatcgc gcaaccagcc
      661 gatcacctcc ttgaggtgag ccgcaccgta cacccgcact ccctcgacca gcgccgcctc
      721 ggcgagtaca gccgtcggca cgaccagccg gtcaatgcct acccggcggg ccgccaagac
      781 ggccggcagc accccacgca ctggtcgcag ccggccgtct agggccagtt caccgatcaa
      841 taccagcccg cgcagccgct cagccggaat ccggcggtcg gcggcaagca aggggcacgc
      901 cagagcgagg tcatagctgc ttccgccctt gggcagggtg gccggcgaca gtgccagggt
      961 catccgtcgc ggtggccacc gctcgcccga gttgagcacc gcagctcgca cccggtccct
     1021 cgcctcggat agggcggtat cgggcaggcc gaccatgtgc agccccggca agccgggacc
     1081 gatgtgagcc tcgatctcca ccagcacccc gtcgaccccg tgtagcgcca ctgcccaggc
     1141 gctggccagc gacatcagaa cgcaccctgc aggtgctcca cggtgaccgg cccatcctgc
     1201 gggcacaaga tcgcgagtac gtcgaagcga acttcgcacc agccgacccg gtaggtccgc
     1261 agccatgttg cagcgacccg ccttatccga gctgccttcg ccggtgtcac gccctccgac
     1321 ggttccccgt accccgtccc cgaccgggtc ttgacctcgc acacgatgag gcgctcgccg
     1381 tcggtggcaa ccagatcaag ctcaccgtcc cggcagcgcc agttgcgcga gagcacgaag
     1441 agaccggcgc ccgccaagta gtccgccgcc agatcctctc cccgcttacc cagcccatcg
     1501 ctatggcggt ccgcagcctg ggtcttcgtc cccattccgg cctccccatt gcctcgtgat
     1561 ctacgacata aatctgccgc gtcagggctg cgccgtcgac agcacgcggg cggacttggg
     1621 gataatgggg caggtgtgga taagtcgggt gccgtaagtc gatggtgcag ttcagtgtcg
     1681 gggtggtgtg ctttgcctac ctcccgaacc tgtggagagt tcgtggcgcg ctgatctagg
     1741 cccggtccgc gaaggtcctt gtcga
//
LOCUS       DATADN010001099         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1330697, whole genome shotgun sequence.
ACCESSION   DATADN010001099 DATADN010000000
VERSION     DATADN010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330697"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1694)
                     /locus_tag="VHH53_14115"
     CDS             complement(<1..1694)
                     /locus_tag="VHH53_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2291292.1"
                     /translation="MTSEVITTDGVALRVYESGPLDAPTVICVHGYPDDHTVWDGVAA
                     ELAPRYRVVSYDVRGAGASGKPRSRPAYRLDQLAEDLAAVVNAVSPARPVHLLAHDWG
                     AVATWHAVTGEWLRGRVASYTSISGPCLDHAAQWFRARLRRPTPRALRELLTQLVGSW
                     YIGFFQLPLLPELAWRTGLGHRALAALEPTGTPAVSDAVHGLQLYRANMPPRLARPRE
                     RTIEIPVQVLAPRRDPFLTVSLQTDVQRWVRNLAVRVLPGGHWLPRTKPDVAARCVSE
                     LIEHAEGGPEGRALRRARVRVSCTTRATGRRRGRFENHLVVITGAGSGIGRACALEFA
                     ECGAEVVATDIDQQAAERTAVLARTLGGRAHAYSVDVSDGGAMEKFATVLREEHGLPD
                     IVINNAGIGLAGPFLDTTVAEWEHIIDVNLWGVIHGCRVLGGLLVEGGAGGTIVNIAS
                     AAAYLPSRTLPAYATTKAAVLALSQCLRAELAEHGVGVVAICPGFVHTNIASTTRFVG
                     LDATAEHTAQQRATALYRRRNFTPQRAAREILRAVERNAAIVPVTPEAKVGLLVSRL"
     gene            complement(1694..>2217)
                     /locus_tag="VHH53_14120"
     CDS             complement(1694..>2217)
                     /locus_tag="VHH53_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEX2291293.1"
                     /translation="YVADIGYAGCLGTNRLHEQLIGDLAEYRALIVRKVNDGLPLQQI
                     YRDVDALIARQGYDNRHRVYPGRVIGHQIGKVRSRLPKFIAAGFGIRSLQTLVGDLIV
                     ERMHHRSPLWADDQISNHPATPGLWAVEPHIGFRDVGVKFEEILVVTGSGSAYWLDDD
                     LPHVHRWQRASAA"
BASE COUNT          356 a          772 c          745 g          344 t
ORIGIN      
        1 agccgggaaa ccagcaggcc aaccttggct tcgggggtga ctgggacgat cgcggcatta
       61 cgctcgactg cccgcaggat ctctcgcgcc gcccgttgcg gggtgaagtt gcggcggcgg
      121 tacagcgcgg tggcccgctg ctgggcggtg tgctcggcgg tggcgtccag gccaacgaac
      181 cgggtggtgc tggcgatgtt ggtgtgcacg aaaccgggac agatcgccac cactcccacg
      241 ccgtgctcgg ccagttccgc gcgcaggcac tggctcagcg ccagcaccgc ggctttcgtt
      301 gtggcatatg caggaagcgt tcgcgacggc aggtaggcag cagcggatgc gatgttgacg
      361 atggtgccgc cggcaccacc ttccaccagc agtccaccca ggacccggca accgtggata
      421 actccccaca ggttgacgtc gatgatgtgt tcccactccg ccaccgtggt gtcgagaaaa
      481 ggcccagcca ggccgatccc agcgttgttg atcacaatgt ccggcaagcc gtgctcttcg
      541 cgcagcacgg tggcgaattt ctccatcgcg ccgccgtccg agacatccac gctgtacgca
      601 tgggcgcgcc cccccaacgt ccgggccagc accgcggtgc gctcggccgc ctgctggtcg
      661 atgtcggtgg cgaccacttc ggcgccgcac tcggcgaact cgagcgcgca cgcgcgcccg
      721 atgccactgc ccgcgccggt gatcaccacg aggtggttct cgaaccgccc acgtcgccgg
      781 ccggtggccc gtgtcgtgca ggacacccgc acccgagcac ggcgtagcgc ccgcccctcc
      841 ggccctccct cggcgtgctc gatcagctcg ctcacgcagc gagcggccac gtccggcttc
      901 gtgcgtggca gccagtgccc gccgggtagc acgcgcactg cgaggttgcg gacccaccgc
      961 tgcacgtcgg tctggagtga caccgtaagg aacggatccc gccggggagc cagtacctgc
     1021 acggggatct cgatggtccg ctctcggggt cgggcaagcc ggggcggcat gttcgcccgg
     1081 tacagctgta aaccatgtac ggcgtcggac acagcggggg tgccggtcgg ctccagggca
     1141 gcgagcgcgc gatgcccgag tccggtgcgc cacgccaact ccggtaacag cggcagctgg
     1201 aagaacccga tgtaccagct gcccaccagc tgggtgagaa gttcacgcag cgcccgtggc
     1261 gtcgggcggc gtagccgcgc ccggaaccac tgggccgcgt gatccaggca cggcccggag
     1321 atcgacgtgt aggaggcgac ccggccgcgc aaccactcgc cggtcaccgc atgccaggtc
     1381 gcgactgctc cccagtcgtg ggccagcaga tgcaccggcc gagccgggct taccgcgttg
     1441 accaccgcag ccagatcctc ggcgagctgg tcgagccggt atgccggccg gctgcgtggc
     1501 ttcccggacg cgccggcgcc acggacgtca tagctcacca cgcggtagcg cggggccaac
     1561 tcggctgcca cgccgtccca cacagtgtgg tcgtcggggt agccgtgcac gcagatcacc
     1621 gtgggtgcgt cgagcggtcc ggactcgtac acccgcagtg ccacgccgtc ggtggtgatc
     1681 acttcgctgg tcatcacgca gccgatgctc gttgccagcg gtgcacgtgc ggaagatcgt
     1741 cgtcaagcca gtaggcgcta ccgctgccgg tcacgacaag gatctcctcg aacttcaccc
     1801 cgacgtcgcg gaagccgatg tgcggctcta ccgcccacaa acccggagtc gccggatggt
     1861 tggagatctg atcatcggcc cacagcggcg agcggtggtg catccgctcc acgatcaggt
     1921 caccgaccag ggtctgcaga gagcggatgc cgaagccggc cgcgatgaat ttcggcagcc
     1981 gcgagcgcac cttgccgatc tggtgcccga tgacccgccc tggatagacg cggtgccggt
     2041 tgtcgtaacc ctgccgggcg atcagcgcat cgacgtctcg atagatctgc tgcagtggta
     2101 gtccatcgtt cactttcctg acgatcagtg cccggtactc ggcgaggtcg cctatcagct
     2161 gctcatggag ccggttggtg cccaagcaac cggcgtagcc gatgtcagcg acgtatc
//
LOCUS       DATADN010001100         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1330711, whole genome shotgun sequence.
ACCESSION   DATADN010001100 DATADN010000000
VERSION     DATADN010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330711"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1056)
                     /gene="aztB"
                     /locus_tag="VHH53_14125"
     CDS             complement(199..1056)
                     /gene="aztB"
                     /locus_tag="VHH53_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease AztB"
                     /protein_id="HEX2291294.1"
                     /translation="MEWLTGPFEVTFVQRALWGGMLVSVICALAGTWVVLRGMAFLGD
                     AMSHGLLPGVALAALLGGNLLVGAVVSAVVMTAGVTALGRTPRLSQDTGIGLLFVGML
                     SLGVIIVSRSQSFAVDLTGFLFGDVLAVREQDLMLLGVALLLALVVSVLGHRAFLALA
                     FDPRKARTLGLRPRLAHAVLLGLLALAIVASFHIVGTLLVLGLLIAPPAAALPWARSV
                     RGVMALAALLGITATFCGLLLSWHLSTAAGATVSALAVSLFFLSHLASGLRRHRRAHF
                     VGVHAAATD"
     gene            1081..1812
                     /gene="aztA"
                     /locus_tag="VHH53_14130"
     CDS             1081..1812
                     /gene="aztA"
                     /locus_tag="VHH53_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HEX2291295.1"
                     /translation="MFTKSLSLAQAEDRTFRVRLAKLSAGYPGRPVLHQLDVQIPALA
                     TTALVGPNGSGKSTLLGVLAGVIQPTSGELHHAGNRPPAFVPQRGAVGDALPLTVRQT
                     VEMGRWGERGPWRRLTRQDRATVDTAMERLGIADLATRQLGELSGGQRQRALIAQGLA
                     QESDLLLLDEPTTGLDPEARERIGVLLTELVADGVTVVQATHDLEAARSADACLLLRD
                     GHLAGQGDPEQVLTASALARIWQPA"
BASE COUNT          335 a          617 c          641 g          223 t
ORIGIN      
        1 cgactgctgc tcggccttct cctgtgcccc ctcgacgtat ccgtgcggag cggatgccga
       61 ggcggttgac ttcggggtgg acacgtcgtc gttctggctg gcgcaactcg tggtgagcag
      121 ggagacggcc acgagcgcgg caggtgccca ggaactgacg tttcttcgga cgagcaaggg
      181 gatcgcctca ggttcttttc agtcggttgc cgcggcgtga acgccgacga agtgcgcacg
      241 ccggtggcgg cggagaccgg atgccaggtg ggagaggaag aacaggctca ccgcgagggc
      301 cgagacggtc gcgccggccg cggtgctcag gtgccaggac agcagcaggc cgcagaaggt
      361 ggcggtgatg ccgagcagcg ccgcgagggc catgacacct cgcacactgc gcgcccaggg
      421 cagtgcggcc gccggcgggg cgatgagcag gcccagcacc agcagcgtcc ccacgatgtg
      481 gaacgaggcg acgatggcca gcgccagcag gccgagcagc acggcgtgag cgaggcgggg
      541 gcgcaggccg agcgtccggg ccttgcgcgg gtcgaaggcc agggcgagga aagcccggtg
      601 gccgaggacc gagacgacca gtgccagcag cagcgccaca cccagcaaca tcaggtcctg
      661 ctcacggacg gcgaggacgt caccgaacag gaacccggtc aggtcgaccg cgaaggactg
      721 tgagcgcgac acgatgatga cgccgaggga cagcatcccc acgaagagca gaccgatacc
      781 ggtgtcctgg gacagcctcg gggtgcggcc gagggcggtg accccggcgg tcatgacgac
      841 cgcgctcacc accgccccga ccagcaggtt gccaccgagc agcgcggcga gcgcgacccc
      901 gggcagcagc ccgtgggaca tggcgtcacc caggaaagcc atccccctga gcactaccca
      961 ggtaccggcc agtgcgcaga tcaccgacac cagcattccg ccccacaggg cccgttgcac
     1021 aaaggtcacc tcgaagggac ccgtcaacca ctccatgcgg tggacattac aatgaaaatc
     1081 atgttcacga aatccctctc tctcgcccaa gccgaggaca ggacgttccg cgttcgtctc
     1141 gccaagctct ccgccggtta cccgggccgt cccgtcctgc atcaactgga cgtgcagata
     1201 ccggcattgg ccactacggc tctcgtcggc ccgaacggaa gcgggaagtc caccctgctc
     1261 ggagtgctgg ccggagtgat ccaacccaca tcaggcgagc tccaccacgc cggcaaccgg
     1321 ccaccggcgt tcgtcccgca gcgcggcgcc gtcggtgacg cactgccgct gacggtcagg
     1381 cagacggtgg agatggggcg ctggggcgaa cgcggaccgt ggcgccgact gacccggcag
     1441 gaccgcgcca ccgtggacac ggccatggaa cgactgggca tcgccgacct cgccacgcgt
     1501 caactcgggg agttgtccgg cgggcagaga cagcgcgccc tgatcgccca agggctcgcc
     1561 caggagtcgg acctgttgct gctggacgaa ccgaccacgg gcctcgaccc cgaggcgagg
     1621 gagcggatcg gggtgctgct gacggagctg gtcgccgacg gagtgacggt cgtccaggcc
     1681 acgcacgatc tggaagcagc acgctcggct gacgcctgcc tgctgcttcg cgacggtcac
     1741 ctggcaggac agggggaccc cgagcaggtg ctcacggcct cggcacttgc ccggatctgg
     1801 caaccggcgt gagctc
//
LOCUS       DATADN010001101         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1199816, whole genome shotgun sequence.
ACCESSION   DATADN010001101 DATADN010000000
VERSION     DATADN010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199816"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VHH53_14135"
     CDS             complement(<1..707)
                     /locus_tag="VHH53_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2291296.1"
                     /translation="MAGISDVLDWAKHQVSSRVPKADLDQRDSDYIREQLPGTWLLAS
                     LYFRADVRGLDRIPRDGPVLLVGNHSGGNLPPDTFVFTLAFCSYFGVERPFYQLAHNL
                     VVSVPGLGWLRKFGTVAANPENARLALESGAALLVYPGGDYEVFRPSWERHRVDFGGR
                     KGYVKLARDAGVPIVPVASVGGQEAALFLDRGQWLARLLMVDKLARLKSVPILLAPPW
                     GLVVSDLIPRLPLPTKIV"
     gene            802..1701
                     /locus_tag="VHH53_14140"
     CDS             802..1701
                     /locus_tag="VHH53_14140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX2291297.1"
                     /translation="MLRPRRFDVEVDPGPVQIQARRVAIDVTGSDLHWIPGHPVASNV
                     VSLLNIVLPAAERWFVDTFNEALPLVRDPRLAEDMRGFIGQEATHADVHEQVLHDYLE
                     TRGIDPKPVLDQIEYVFGRMLAPSTSTDPKRRLNHLCDRLWLIAAIEHYTAVMGDFAL
                     NCAWDDFGADPTMVDLFRWHGSEEVEHRSVAHDVAVYFHDSYLDRIRAMSIAVVMIFV
                     FFQRAAWFLVKRDPNTDIGWWRFNRLRMRDSKLGLLPRYRKLFGGNTLMYFRPGFSPE
                     EMGSTAQAVAYLASSPAARAAHI"
BASE COUNT          297 a          601 c          545 g          261 t
ORIGIN      
        1 acgatcttcg tcggcagcgg cagccgtgga atgaggtcac tgaccaccag accccaaggc
       61 ggcgccagca gaatcggcac gctcttcaac cgcgccagct tgtccaccat cagcaaccgc
      121 gccagccact ggccgcggtc gaggaacaac gccgcctcct gaccgccgac actggcgacc
      181 ggcacgatcg gcacgcccgc gtcccgggcc agcttcacgt agcccttccg gccgccgaag
      241 tcgacccggt gccgctccca cgacggtcgg aacacctcgt agtcgccgcc gggatagacc
      301 agcagcgcgg caccggattc cagcgccagc cgcgcgttct cgggattggc cgccaccgtg
      361 ccgaacttgc gcagccaccc caggccgggc acggacacca ccaggttgtg cgccagctgg
      421 tagaacggcc gctcgacacc gaagtacgag cagaacgcca gtgtgaacac gaatgtgtcg
      481 ggcggcagat tgccgccact gtggttgccg accagcagca cgggcccgtc gcgagggatg
      541 cggtccagcc cccgcacgtc ggcccggaag tacagcgagg cgagcaacca ggtgccgggc
      601 agctgctcac ggatgtagtc cgaatcgcgc tggtccaggt ccgccttggg caccctggac
      661 gacacctggt gcttggccca gtcgagaacg tcgctgattc ccgccatggc acgtcgtatt
      721 gacaacccgg ggttagccga aacctgcgtt cccacctaac cgtcgtagac tgcgggcgtc
      781 actggcatta ggaggtgtgc gatgttgcga ccgcgccgct tcgatgtgga ggtcgatccg
      841 gggcccgtgc agatccaggc ccgaagagtg gccatcgacg tcaccggcag cgacctgcac
      901 tggatccccg gtcatccggt ggcgtccaac gtcgtcagcc tgctcaacat cgtgctgccc
      961 gccgccgaac gctggttcgt cgacaccttc aacgaggcgc tgccgctggt gcgggatccc
     1021 cggctggccg aggacatgcg cgggttcatc ggccaggaag ccacccacgc cgacgtgcac
     1081 gagcaggtgc tgcacgacta cctcgagacc cgcggcatcg accccaagcc ggtcctcgac
     1141 cagatcgagt acgtgttcgg ccgcatgctg gcgcccagca cgtccaccga tccgaagcgc
     1201 aggctcaacc acctgtgcga ccggctgtgg ctgatcgcgg ccatcgagca ttacaccgcg
     1261 gtcatgggtg acttcgcgct gaactgcgcg tgggacgact tcggcgccga cccgaccatg
     1321 gtcgacctgt tccgctggca cggcagcgag gaggtcgagc accgcagcgt cgcccacgac
     1381 gtcgcggtct acttccacga cagctacctc gaccggatcc gggcgatgtc catcgcggtg
     1441 gtgatgatct tcgtgttctt ccagcgcgcc gcctggttcc tggtgaagcg ggacccgaac
     1501 accgacatcg gatggtggcg gttcaaccga ctccgaatgc gcgactcgaa gctgggtctg
     1561 ctgccccgct atcgaaagct gttcggcggc aacacattga tgtacttcag gccgggcttc
     1621 tcgccggagg agatgggatc gacggcgcag gcggtcgcct acctggccag ctcaccggcg
     1681 gcgcgggccg cgcacatctg atgc
//
LOCUS       DATADN010001102         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_593032, whole genome shotgun sequence.
ACCESSION   DATADN010001102 DATADN010000000
VERSION     DATADN010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593032"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            524..874
                     /gene="ssb"
                     /locus_tag="VHH53_14145"
     CDS             524..874
                     /gene="ssb"
                     /locus_tag="VHH53_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2291298.1"
                     /translation="MTEAAVSFAGNLTDDPEVRYTEAGIARAMFRVAVSSRREQEASF
                     FNVIVWRDQAEHAAQSLAKGSRVVVVGRLQQRSWTAEDGSARSAVEVVAEELKPSLRW
                     ATATTNWATRSSSQ"
     gene            1229..1942
                     /locus_tag="VHH53_14150"
     CDS             1229..1942
                     /locus_tag="VHH53_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEX2291299.1"
                     /translation="MPIDPAALARNLSTLAALDAEHDLARALQQIASAAKVLLRVDGA
                     GLMLADARGELRWATASDQQTQVIEEGEERLGQGPCVNAFAEHAPMAMRDATKEPQWG
                     KITDVVTAQEMRAGLSVPVQLEGGPIGTLDLYSAAPRDWDQAEISAAQVYAALAATLL
                     CQAAAAQVKGRLADQLQVAFEHRTRIERAKGMVMLQEGIDDAAAFERLRSAARSSRRP
                     LIEVVDEVLAGQRLSRPRR"
     gene            2202..>2634
                     /locus_tag="VHH53_14155"
     CDS             2202..>2634
                     /locus_tag="VHH53_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291300.1"
                     /translation="MAIAPRQRLVNTALTALLVVGAVLVLFDLARVVFLDLARSDRPS
                     DRRCAELWNGPANQTNRSLVRGRAFLLATVRGVEPNKAGQPGCGVLLRERADGPWLSF
                     SVVLTETAVYWDPGVSGVRYGTDSPTGYWGDPPNATLQADGS"
BASE COUNT          440 a          873 c          923 g          398 t
ORIGIN      
        1 ccggcgtgct ggcatgagct cttgtcgtcg ccgcaacgcc ggaggaaagt acgcccatgg
       61 cgaaggacat tcggtgccga ctccgccttc accgctatcg gccgaagaag aatgatggtg
      121 gtgaggtgta ctgggaatgc acccgctgcg gccacatgaa ggcctatgac tcagtcgacc
      181 ctggtgcggc cgggtctggc tatggcatcg gtaactaacg gttagcccgg cagcctgagc
      241 cgagttgcaa gcaactctcg tggcgggagc ctcgttctcc cgccacgccc agggtggagg
      301 gatctggccg gacggtactg gcatgcccaa ccaccagccg caagcacccc caaccaactc
      361 ggctcctgag ccgaccgatg gccggcgagt tgatgcatgg gccgacttcg ttggctctgc
      421 tgcttgcccc tggcggcctt ccggcatgcc acggtgtcca ccgtcccccg gttccaaggg
      481 ctgggcagac cgcccggcca ggcaacagaa aggggcagga cccatgaccg aggccgccgt
      541 gagcttcgcc ggcaacctca ccgacgaccc cgaggtccgc tacaccgagg cagggatcgc
      601 ccgggccatg ttccgggtgg ccgtctccag ccgccgggag caggaggcgt cattcttcaa
      661 cgtcatcgtg tggcgcgacc aggccgagca tgccgcgcag tccctggcga agggtagccg
      721 ggtggtggtc gtgggccgcc tgcagcagcg gagctggacg gctgaggacg gcagcgcccg
      781 atccgccgtc gaggtcgtgg ccgaggagct gaaaccgagc ctgcggtggg cgacggcgac
      841 cacgaactgg gcgacgagga gctccagcca gtagccagct cgaccaggcg atgcgacgag
      901 cgggacgcgg ccagcatgct cgcgtccgct tcgtcgcgtc cgtactaggg agagatgaag
      961 ctgccccggg cgccgacaga ggcaagcgag gtctaggcct gccactcccc acgaggctgg
     1021 taccggctcc ggtgcagagg tcagccgccc ggggcagcca cgcttggtgt atccctcctc
     1081 gccgggctcc aaccgggcac ggcaacaaca cagtgcatgg cgggttgagt ggatgcgcgc
     1141 ttgtcgtaaa ctgccggcta tacgaagctc agtccttttg ggccagacgc accgccgcgc
     1201 ctcccctgat ccggtgaagg agatcggcat gccgatcgat cctgctgccc tggcccgaaa
     1261 cctcagcaca ctagccgccc tggacgccga gcacgatctc gcgcgggcgc tccagcagat
     1321 cgccagcgcc gccaaggtgc tgctgagggt ggacggggcc gggctgatgc tggccgatgc
     1381 gcggggcgag ttgcgttggg cgaccgcctc cgaccagcag acccaggtca tcgaggaggg
     1441 cgaggagcga ctggggcagg gtccctgcgt caacgcgttc gccgagcacg ccccgatggc
     1501 gatgcgggat gcgaccaagg agccgcagtg gggcaagatc accgatgtgg tgaccgccca
     1561 ggagatgcgg gccggcctca gcgtcccggt gcagctagaa ggcggcccga tcggcaccct
     1621 ggacctgtac tcggctgcgc ccagagactg ggatcaggcg gagatcagcg cggcccaggt
     1681 gtatgcggcg ctggcggcga ccctgctttg ccaggccgct gccgctcagg tcaaggggcg
     1741 gctggccgac cagttgcagg tggcgtttga gcaccgcacc cggatcgagc gggccaaggg
     1801 gatggtgatg ctgcaagagg ggatcgacga cgcggccgcg ttcgagcgcc tgcggagtgc
     1861 ggctcggtcc tcacggcggc cgctgatcga ggtggtcgac gaggtgctcg ccggtcagcg
     1921 gctctcccgc ccgcgaaggt aaagtcgcct ggctggccgg tcggtggtcg acagtaccag
     1981 cagactaagc cgagggtgtc gaccttcagc cgagacactg gaaaccagca ggtgaggcga
     2041 cctgtcaagc atcaacctca ccagcacacg tgctggtgag gttcgtcgtg gagtttctct
     2101 acgaactcaa ggccggcaga ccatcggcct actgaaccaa acgtctcggc cgcccgtcga
     2161 agcaagtaga atccgggaac tcgcgcagca gggggtccgg catggccatc gcgccacgtc
     2221 agcggctcgt caatacggcc ctgacagccc tcctcgtcgt cggtgccgtg ctcgtgctct
     2281 ttgacctggc ccgtgtcgtg ttcttggacc tggcccgttc cgaccggccc tcggatcgtc
     2341 gctgcgctga gctgtggaac ggaccagcca accagaccaa ccgatccttg gtccgcggcc
     2401 gcgccttcct gctggccacg gtccggggtg tcgaacccaa caaggccggc cagccgggct
     2461 gcggggtgct gttgcgggaa cgcgcggatg gaccctggtt gtcgttcagc gtcgtcctca
     2521 ccgagaccgc ggtgtactgg gaccctggcg tgtccggcgt tcggtacggg accgacagcc
     2581 cgaccggcta ctggggcgac cctcccaacg cgacactgca ggcggacggg agcc
//
LOCUS       DATADN010001103         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_593534, whole genome shotgun sequence.
ACCESSION   DATADN010001103 DATADN010000000
VERSION     DATADN010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593534"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2499)
                     /gene="rpoB"
                     /locus_tag="VHH53_14160"
                     /pseudo
     CDS             complement(1..2499)
                     /gene="rpoB"
                     /locus_tag="VHH53_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453211.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT          482 a          923 c          923 g          533 t
ORIGIN      
        1 gccggtctcg ccgtgcggca ccttgaggct ggtgtcgcgg acctcgcgcg ccttctcacc
       61 gaagatcgcc cgcagcagcc gctcctccgg ggtcagctcg gtctcgccct tcggggtcac
      121 cttgccgacc agaatatccc cggcctgcac ctcggccccg atccggatga tgccacgctc
      181 gtcgagatcc gccagcactt cctcagagac gttcgggata tcccgagtga tctcctcggc
      241 gccgagcttg gtgtcccggg cgtcgatctc gtgctcctcg atgtggatcg aggtgagcac
      301 gtcgtcctgg accaggcgct ggctgaggat gatcgcgtcc tcgtagttgt ggccctccca
      361 cggcatgatc gccacgagca gattcttacc cagggccatc tcgccggagt cagtgcacgg
      421 cccgtcggcg atcacctgac caatgtccac ccggtcgccc tcggccacga tcggccgctg
      481 gttggtgcag gtgccctggt tggaccggtg gaacttgtgc atccggtagg tctgccgact
      541 gccgtcgtca gccatgacgg tgacgtagtc ggcacacagc tcctcgacca cgccggcctt
      601 gtcggccacc acgacatcgc cggcatccac cgcagcccgc agttccatac cggttcccac
      661 cagcggcgcc tcgctgcgca gcaacggcac tgcctggcgc tgcatattgg cgcccatcag
      721 tgcgcggtta gcgtcgtcgt gctcgacgaa cgggatcatc gcggtggcca ccgagaccat
      781 ctgccgcggt gacacatcca tgtagtccac gccgtgcggg tcgatgtagt cgacatcgcc
      841 gcccttgcga cgaaccagca cgcggtcctc ggcgaagtgg ccgtcctcgg ccagcaccgc
      901 gttggcctgc gcgatgacgt gccgatcctc ctcatcagca gtgaggtagt cgatctgctc
      961 ggtgaccaga ccgtcgacga ccttgcggta gggcgtctcg atgaaaccga acgggttgac
     1021 ccgggcataa gaggacagcg agccgatgag accgatattc gggccctccg gggtctcgat
     1081 cgggcacatc cggccgtagt gcgaatggtg aacgtcgcgg acctcgaagc cggcccgctc
     1141 ccgggacaga ccaccaggac ccagggcgga cagccgccgc ttgtgggtca gcccggccag
     1201 cggattggtc tggtccatga actgggacag ctgggaggtg ccgaagaact ccttgatcgc
     1261 cgctaccacc gggcggatgt tgatcagggt ctgcggcgtg atggcctcga cgtcctgggt
     1321 cgtcatccgc tcgcgcacga cccgttccat gcgggacaga ccgacccgga tctggttttg
     1381 gatcagctca cccacggtgc gcaacctgcg gttaccgaag tggtcgatgt cgtcggtctc
     1441 caccgggacc acggcttccg gaccgccact ggccgcaggc atcgtcgtct agcctgcgtg
     1501 cagccggacc aggtagtcga tggtggtgac gatgtcgtcc tcggtgagca ccccgttgcg
     1561 atgcggcagg gccagaccca actttttgtt gaccttgtaa cggccgacct tggcaaggtc
     1621 gtaacgcttg accttgaaga agaggttctc cagcagcgct tgggcgcttt ccttggtcgg
     1681 cggctcaccc gggcgcagct tgcggtagat gtccagcagc gcctcatcgg ggccgccggt
     1741 gtgatccttt tccagggtgg cgagcagcgt ctcggagtgc gcgaatcgag cggcgatctg
     1801 ctcggtcgtc cagcccagtg ccttgaggag taccgtcacc ggctggcgac gcttgcggtc
     1861 gattcgcacg cccactgtgt cgcgcttgtc cacgtcgaac tccagccatg caccacggct
     1921 gggaatgatc tttacgctgt agacgtcctt gtcggtggtc ttgtccaccg cgtggtcgaa
     1981 gtacaccccc ggagagcgga cgagctggga caccacgacc cgctcggtcc cgttgatgat
     2041 gaaggtgcct ttgctcgtca tcatcgggaa gtcacccatg aacaccgtct gagacttgat
     2101 ctcaccggtg ttgttgttgg tgaactcggc ggtgacgaac aagggcgccg cgtaggtcat
     2161 gtccttgtct ttgcactcct ccacggaggc cttgacctcg tcgaaacgag gatcggagaa
     2221 tgacagggcc atcgagccgg agaagtcctc gatcggcgag atctcgtcga gtacctccgc
     2281 gaggccaccc accggttcgt cgtcaccggc ctcgatgcgg cgctggaacc attcgtcggc
     2341 accaaccagc cactcgaacg attgaagctg gagatccaga aggtcgggca cctcgagcgg
     2401 ctcgcggatc ttcgcgaacg atggccgggt gggggctccc ggaaggcggg gtacggaggt
     2461 attcgcagca gaggacttgg tcgggcggga gactgccaag atgcgtcctt ccgaacacta
     2521 gcgctgccga gcgcaggaac gcatggtccg cgctgaccac gctgcctgcg ctgtagttcg
     2581 tgcagttact gatgcagtta tggcgatact tgtggggata ctgtaggtag tgccttgaag
     2641 tagagcggct ggtgcggtca cgctgcccta ctgttcaggc aggccccagc gccccggagt
     2701 gcacggggaa acaaggagag ggcagcgcaa agaagcagtc taaccgctag gacggcgact
     2761 gtccagcacc gggcgcaacc cggtgcaagc gtgctcctag agtgacctgc ggcacgccgc
     2821 ccgtcaagag tgcatcgtcc gatcagtggt cggatacgca c
//
LOCUS       DATADN010001104         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_739693, whole genome shotgun sequence.
ACCESSION   DATADN010001104 DATADN010000000
VERSION     DATADN010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739693"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1626
                     /locus_tag="VHH53_14165"
     CDS             247..1626
                     /locus_tag="VHH53_14165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2291301.1"
                     /translation="MSNRLIFQLGTNNWQRGGEFAPGSGILHEAHHHAYNALPGVRAY
                     SMYPSRRQRFRDEDVRVFALEHDIPICESISPVSNYRWHSMSEDEVNAYRQRLTNEVA
                     EWLDEIEANSGDRFSLAIAHHGFMNTVVMRDVIRRRAVAGRGSMPLLCFVHGTELKMY
                     ANERRADQPAEFPLRFLPFMQHEKIFDYNAPQHAVDVVASISAEQIEALLDLFPEFPR
                     ERVVLSQNGYNQDVFHRLTEPTDLYADRAKVLAGFMTQVPEGSGRLPEPIAPADGFDA
                     VVAFCGKFADWKRLDVLLRAAAIYEQQERRILTLVIGSGPHEAQVEMNDLAARLGVRR
                     VYFLGPRPQDELAVLFNCADVGCFPSYREPFGLVFIECMACGTPVIGADSGGPRDFVT
                     PKVGMLVPETDDRAALAQSLAAAVDRGLDEGWKRTKGATAEAYAKENFSVVSQVSKLL
                     AEVDRLIAT"
     gene            1654..>1836
                     /locus_tag="VHH53_14170"
     CDS             1654..>1836
                     /locus_tag="VHH53_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291302.1"
                     /translation="MAVSGDGRTRSVAVPMSATAMPQDHKLGGSETGAVALISCRSGR
                     GLGYVVRSLEAALWAFD"
BASE COUNT          303 a          612 c          622 g          299 t
ORIGIN      
        1 cgcgtctgaa acacggtagc ccatgatcac cgtcatgtga cccgccggca ccgggagcga
       61 ccggggtgcg cgcctgcacc gtcaccggca ctgcccggtc acctcgcgca atcaagccca
      121 cggcccccga tcatgctcga gggcgtgatc gatcggccct actgggcgtc tcgctttcct
      181 tttccgccga acccggccat gatcgcggct cgcgggagca gacaaggttc gagcgcggag
      241 gcgaggatgt ccaatcggct gatctttcag ctgggcacta acaactggca gcgcggcggg
      301 gagttcgcgc cgggctcggg gatcctgcac gaggcgcacc accacgccta caacgctctg
      361 ccgggagtgc gtgcctactc gatgtatccc tcccggcggc agcggttccg cgacgaagac
      421 gtgcgggttt ttgcgttgga gcacgacatc ccgatctgcg agtcgatctc cccggtcagc
      481 aactaccgct ggcacagcat gagcgaagac gaggtcaatg cctaccggca gcggctgacc
      541 aacgaggtcg cggagtggct cgacgagatc gaggccaact ccggggaccg attcagccta
      601 gcgatcgccc atcacggctt catgaacacg gtggtgatgc gcgacgtgat ccgccgcagg
      661 gccgtcgccg ggcgcggctc gatgccgctg ctgtgcttcg tgcatggaac cgagctgaag
      721 atgtacgcca acgagcgacg cgccgatcag ccggcggagt tcccgctgcg cttcctgccg
      781 ttcatgcagc acgagaagat cttcgactac aatgccccgc agcatgccgt ggatgtcgtc
      841 gcgtcgatct ccgccgagca gatcgaggcg ctgctggacc tcttcccgga gttcccccgt
      901 gagcgggtgg tgctctccca gaacggctac aaccaggacg tcttccacag gctgaccgag
      961 ccgaccgacc tctatgctga tcgagcgaag gtgctggccg ggttcatgac gcaggtaccc
     1021 gagggcagcg gccggctgcc cgagccgatc gccccggcgg acgggttcga cgcggtggtg
     1081 gcgttctgcg gcaagttcgc cgactggaaa cgactggatg tcctactgcg cgcggcagcc
     1141 atctacgagc agcaggagcg ccggatcctc accctggtga tcggttccgg gccacacgag
     1201 gcgcaggtcg aaatgaacga cctggccgcg cgccttggcg tgcgccgggt ctacttcctc
     1261 gggccgaggc cgcaggacga gttggcggtg ctgttcaact gcgccgacgt gggctgcttc
     1321 ccgtcctacc gtgaaccttt cggtctggtg ttcatcgaat gcatggcctg cgggacgccg
     1381 gtgatcggcg ccgactccgg cggcccgcgc gacttcgtga cccccaaggt cgggatgctg
     1441 gtaccagaga ccgacgatcg cgcggcgctc gcccagtcac tcgccgccgc cgtcgaccgg
     1501 ggtctggacg agggctggaa acggaccaag ggcgccaccg ccgaggccta tgccaaggag
     1561 aacttcagcg tggtcagcca ggtctccaag ctgctcgccg aggtagaccg gctgatcgcc
     1621 acctgaggat cacacaccga ccgggtgcgc gcggtggcgg tcagcggcga cgggcgcacc
     1681 cggtcggtgg ccgtccccat gtccgccacc gccatgccgc aagatcacaa actaggaggg
     1741 tcggagactg gcgcggtggc gttgatcagc tgtaggtctg ggcgcggcct ggggtacgtg
     1801 gtgcgctcgc tcgaagcggc gctctgggcc tttgac
//
LOCUS       DATADN010001105         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_121472, whole genome shotgun sequence.
ACCESSION   DATADN010001105 DATADN010000000
VERSION     DATADN010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121472"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..922
                     /locus_tag="VHH53_14175"
     CDS             join(25..371,370..922)
                     /locus_tag="VHH53_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558180.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2291303.1"
                     /translation="MTREPVLSDEMWARVEPLLPPVKGSRGRPMRDHRQLVEGAIHHY
                     PTGVAWRDLPAEFGPWQTVWKRHRRFAADGTWDRVHGALLADADAAGLIDWDVSVDST
                     INRAHQHATNTKRGARGAPENYKNPLDEPDDHAIGRSRGGLSSKIHHLVDGRGLPLVV
                     LVGAGQAGDAPMFPILLHHLRVPRIGPGRPRTRPDTVAGDKAYSSRAIRTHLRERGIG
                     ACIPEPDDQKGHRKRRGSRGGRPPAFDAERYRGRNVVERAFNEDKQWRSLATRYDKLA
                     IVYRAAAVLRAVTLWLKRLGDMP"
     gene            955..1410
                     /locus_tag="VHH53_14180"
     CDS             955..1410
                     /locus_tag="VHH53_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291304.1"
                     /translation="MLLLDEPSNGLDPDGIRWLRDYLADFATRGGTVFVSSHLISELS
                     MFAHDLVVVGGGRLLAAESVQAITGRNDIDVRVETPQPTELLEHLAAAGIAAEVRADR
                     LILRGTTRAAVSQIAFDNRIHVLELTDVSRSLEDSLLDMTRSSAEFASL"
     gene            1444..>1808
                     /locus_tag="VHH53_14185"
     CDS             1444..>1808
                     /locus_tag="VHH53_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291305.1"
                     /translation="MFTTSAPAPATRTRGDDVRAVVAALRSDWIKASTVRANRAILGL
                     TVVGGLLVSWAVSVLVTDQVQYVAQVGFFWTSVSSMLAAITGVLLFGSEVQHGTLAAA
                     VAARPSRWIVALAKTLTAAA"
BASE COUNT          275 a          655 c          603 g          275 t
ORIGIN      
        1 ggttcgggag ctgatgctgg gcaaatgacg cgtgagccgg tgctcagtga cgagatgtgg
       61 gcgcgtgtgg agccgttgct gccgccggtg aaggggtctc gggggcggcc gatgcgggat
      121 caccgccagc tggttgaggg ggctatccac cactacccca ccggtgtggc ctggcgtgat
      181 ctgccggcgg agttcgggcc gtggcagacg gtgtggaagc ggcatcgccg cttcgccgcc
      241 gatggcacct gggatcgggt gcatggggca ctgttggccg atgccgacgc tgccgggctg
      301 atcgactggg acgtgtcggt ggactccacg atcaaccgcg cgcaccagca cgcgacgaac
      361 accaagcggg gcgcgggggg caccggagaa ttacaagaac ccgctcgacg agcctgatga
      421 tcatgcgatc gggcggtccc gcggcggcct gagcagcaag atccaccacc tggtcgatgg
      481 gcgcgggctg cccctggtcg tgctggtggg cgcggggcag gccggtgatg ctccgatgtt
      541 cccgatcctg ctgcaccacc tgcgagtgcc tcgaatcggc ccgggacggc cacgaacccg
      601 gcccgacacc gtggccgggg acaaggctta ctcctcgcgc gcgatccgca cccatctgcg
      661 tgagcgtggc atcggcgcct gcattcccga accggatgac cagaagggcc accgcaagcg
      721 tcggggcagc cgcggaggac gaccaccggc cttcgacgct gagcgctacc gcggacgcaa
      781 cgtcgtcgag cgcgccttca acgaggacaa acagtggcgc tcactggcca cccgctacga
      841 caagctcgcc atcgtctacc gggctgcagc agtcctacgc gcggtcaccc tctggctcaa
      901 gcggttagga gacatgccct agctctggcg tcggcgctgc tcggcgagcc ccaggtgctg
      961 ctgctcgacg agccctcgaa cggtctcgac cccgacggca tccgctggtt gcgcgactac
     1021 ctggccgact tcgccacgcg cgggggaacg gtgttcgtct ccagccacct catcagcgag
     1081 ctcagcatgt tcgcccacga cctcgtcgtc gtcgggggag ggcggctgct ggccgccgag
     1141 tcggtgcagg ccatcaccgg ccgcaacgac atcgacgtgc gcgtcgagac gcctcagccc
     1201 accgaactcc tcgagcacct cgccgccgcc ggcatcgccg ccgaggtgcg ggccgaccgc
     1261 ctgatcctcc gggggaccac ccgagcagcc gtctcgcaga tcgcgttcga caaccggatc
     1321 catgtcctcg agctgaccga cgtctcccgc tcgctcgagg acagcctgct cgacatgacc
     1381 cgctcgtccg ccgaattcgc ctcgctctga tccgccgtcc accactcgag agggactgaa
     1441 cccatgttca ccacatcagc acctgcaccc gccacccgca ccaggggcga cgacgtccga
     1501 gccgtcgtcg cggctctgcg cagcgactgg atcaaggcgt ccaccgtccg ggccaaccgg
     1561 gcgatcctcg gcctcaccgt cgtcggcggg ctgctcgtct cctgggccgt ctcggtcctg
     1621 gtgaccgacc aggtccagta cgtggcccag gtcggcttct tctggaccag cgtgtcctcg
     1681 atgctcgccg caatcaccgg ggtgctgctc ttcggctccg aggtccagca cggcaccctg
     1741 gcagccgcgg tcgccgcacg cccctcgcgc tggatcgtcg cactggccaa gacgctcacc
     1801 gcggccgc
//
LOCUS       DATADN010001106         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1331703, whole genome shotgun sequence.
ACCESSION   DATADN010001106 DATADN010000000
VERSION     DATADN010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331703"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="VHH53_14190"
     CDS             complement(<1..1609)
                     /locus_tag="VHH53_14190"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HEX2291306.1"
                     /translation="MATAEQPVATGMLSVTNLAPDGSPLGGGCFTLTDVANVPVSEVC
                     DNDASDFDSAPGVIAFGGVPAGQYTLTQTAAAESFSPAAPLAVDHGVDDTVVEMVSQP
                     ATEETGVVELVTFGEDGNPIGGECYTLVGVTESFGPFCDNGEGDTSADPGLLTVEKLP
                     VGTYEAVLELAVEDADVEQAQQAKPRRSVSVRRGDRPTRAIFNVRAQQRQRGDLLIRV
                     RNQDGNYLEGACFALIPDGDTAPSAEVCDNRGEDQNSADGRILMTNNRAGRYTLTQTT
                     APTGYSTAADQALRIAAGSVREVAVTNEPEPERTATLDVRTADGEGNVLPGACYAITR
                     GNAPLEDCDDDDDGITRFAGIPSGSYVVRQTQPPAGGYTTAGSTATRLDPGQTATVTV
                     VNEARPGSLVLRKTDNTGQLLAGACFALRSGDRAVYSICDNDASDANTSVGVILLGTV
                     APGTYTLRETQPPVGYLSAADQEVTISANQRTQVSIVNAIAPAPERSGDLRVFKLDSN
                     GRALAGSCFALVDGDGQVLAPRCDADDG"
     gene            1784..2059
                     /locus_tag="VHH53_14195"
     CDS             1784..2059
                     /locus_tag="VHH53_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291307.1"
                     /translation="MPILAGTGLDDRVVPRREIREVQSAIVALVADVCQCLGLGRSPE
                     GEAGSADALTVFVIDEDDQGARIRGNREINSADFTRLDVNILDAGIH"
BASE COUNT          311 a          665 c          695 g          400 t
ORIGIN      
        1 ccccgtcatc ggcgtcacag cgtggcgcga gcacctgacc atccccgtcg acgagggcga
       61 agcaggaccc agccagtgcc cggccgttgc tatcgagctt gaagacccgt agatcgccgc
      121 tcctctccgg tgccggcgca atggcgttga cgatactcac ctgggtccgc tggttggcgg
      181 agatggtcac ctcttgatca gccgcgctca gatagccaac cggcggttgg gtctcgcgca
      241 gcgtgtacgt tccaggcgcg actgtcccca gcagtatgac gccgacgctg gtgttggcgt
      301 cgctggcatc gttgtcgcag atcgagtaga cagcgcggtc tccactacgg agggcgaaac
      361 aggctcccgc cagaagctgg cccgtgttat cggtcttgcg caacaccagg ctgcccgggc
      421 gtgcctcatt gacgacggtg accgtcgcgg tttgcccagg gtcgagacgg gtggccgtgg
      481 agccggctgt ggtgtaacca cccgcgggcg gttgggtctg acgcacgacg tagctgccag
      541 atggaatacc ggcgaaccgg gtgattccgt catcatcgtc gtcgcagtct tcgaggggcg
      601 cattcccccg cgtgatcgca tagcaggctc cgggcagcac gttcccttcg ccgtccgcag
      661 tcctgacgtc gagggtcgcg gttcgctctg gttcaggctc gttcgtgact gccacctcac
      721 gcacggatcc cgccgcgatg cggagagcct ggtcggcggc ggtggagtat ccggtgggag
      781 cggtggtctg ggtgagcgtg taccggccag cccggttgtt cgtcatgagg atgcgaccat
      841 cggcactgtt ctgatcctcc cctcggttgt cgcacacctc tgcggatggc gcggtgtcgc
      901 catccgggat caacgcgaag caggctccct ccagatagtt gccatcctgg ttgcggaccc
      961 ggatgaggag atcgccccgt tgccgctgct gggcgcgaac attgaagatg gcgcgggtcg
     1021 gccggtcgcc gcggcgcacc gacaccgacc gacgcggctt ggcctgctga gcttgctcga
     1081 cgtcggcgtc ttcgacggcc aactccagca cggcctcata cgttccgacg ggcagctttt
     1141 cgaccgtgag caggcccgga tcggctgagg tgtcgccttc tccgttgtcg cagaaggggc
     1201 caaagctttc ggtgactccg acgagggtgt agcactcgcc cccgatcggg ttgccatcct
     1261 cgccgaacgt aaccagctcg accacacccg tctcttcagt cgcgggctgg ctcaccatct
     1321 cgacgacggt gtcgtcgacg ccgtgatcga ctgccagcgg agcggccggg ctgaaggact
     1381 ccgcggccgc ggtttgggtc agcgtgtact ggccagcggg gacaccgccg aaggcgatga
     1441 ctcccggtgc actgtcgaaa tcactggcgt cgttgtcgca gacctcacta accggtacgt
     1501 tcgcgacatc ggtcagcgta aagcagccgc caccgagcgg gctgccgtcc ggcgccaggt
     1561 tggtgaccga tagcatcccg gttgcgacgg gctgctcggc ggtcgccacc ggctgcgacg
     1621 ttgatggtgt ctccgcggtt gcttccggct ccggcgtggc ggttggggtc tgctcgggct
     1681 gaggcgttgg agtcggcgtc tccggttctg gctctcgcgg attgaggaga gtcacttcaa
     1741 cggtctgccc cggctccacg gtcataggct gctcaggcgc caattgccga ttctcgccgg
     1801 cacgggtctg gacgatcgtg tagttccccg cagggagatc cgggaagttc aatcggccat
     1861 cgtcgccctg gtcgcagacg tctgtcagtg tttgggcctc ggtcgctccc cggagggtga
     1921 agcaggttcc gccgatgcgc tcaccgtctt cgtcatcgac gaagacgacc agggtgccag
     1981 gatccgcggt aaccgtgaaa tcaacagtgc ggatttcacc cgcctcgacg tcaatattct
     2041 ggacgccggg attcattagt ccgtctggca g
//
LOCUS       DATADN010001107         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_447497, whole genome shotgun sequence.
ACCESSION   DATADN010001107 DATADN010000000
VERSION     DATADN010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447497"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VHH53_14200"
     CDS             complement(<1..1144)
                     /locus_tag="VHH53_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX2291308.1"
                     /translation="MSEFILAQGGGLELSVDDYTVVGVVAAVALAALAVGAVLLREVL
                     AAGQGTSRMQEIGRAIQEGASAYLNRQFKTLSVFAVIVFLLLFALPAEDLSERIGRSI
                     FFLIGAVFSASIGYLGMWLSTRANIRVAAASNESGGREKAMRIAFRTGGVVGMATVGL
                     GLLGAALVVMVYTGQAPRVLEGFGFGAALLAMFMRVGGGIFTKAADVGGDLVGKVEAG
                     IPEDDPRNAATIADNVGDNVGDCAGMAADLFESYAVTLVAALILGTAAFGLKGLLFPL
                     IVPAIGVVTAVIGVYLTRARAGEPGLRAINRSFYISAAISAVACTVAALLYLPGSFGE
                     LTGVTEIDGEPLSAVPGNPALIAAIAVIIGIVLAGVILWLTGYFTGT"
     gene            1431..>2252
                     /locus_tag="VHH53_14205"
     CDS             1431..>2252
                     /locus_tag="VHH53_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2291309.1"
                     /translation="MQGLLCGSQGSDSPLRHVATIPARAATTVQWPGWVPDQLREALG
                     VAGVAQPWAHQVAVAELARADRHVVVATGTASGKSLAYQLPVLTGLLTDQQATALYLA
                     PTKALGADQIRAVTALGLDGVRPAGYDGDTPMAERDWVRSHARWVFTNPDMLHHGLLA
                     RHDRWARLLRRLRFVVIDECHAYRGVFGSHVALLLRRLRRVAARYGAHPVFVLASATV
                     ADPGASATRLTGLDTVAVIEDSSPRGERTVALWEPPLLPELTGENGAPLRRSAAAE"
BASE COUNT          368 a          783 c          779 g          322 t
ORIGIN      
        1 cggtgccggt gaagtagccg gtgagccaca ggatgacccc ggccagcacg atgccgatga
       61 tcaccgcgat ggccgcgatg agcgccggat tgcctgggac cgcgctgagt ggctcaccgt
      121 cgatttcggt gacaccggtg agctcaccga agctgccggg tagatacagt aaggcggcca
      181 ccgtgcaggc caccgccgag atcgccgcgg agatgtagaa ggagcggttg atcgcgcgca
      241 gtcccggttc gccggcccgg gcccgggtga ggtagacgcc gatcaccgca gtcaccacgc
      301 cgatggcggg cacgatcagc gggaacagca accccttcag gccgaatgcc gcggtgccca
      361 ggatcagcgc cgcgaccagg gtcaccgcgt aggattcgaa caggtcagca gccatgcccg
      421 cacagtcacc gacgttgtcc ccgacgttgt cggcgatggt ggccgcgttg cgcggatcat
      481 cctcggggat gcccgcctcg accttgccga ccaggtcgcc acccacgtcg gcggccttgg
      541 tgaagatgcc gccgccgacc cgcatgaaca tcgcgagcag cgcggcgccg aagccgaagc
      601 cctccagcac ccggggcgcc tgaccggtgt agaccatcac caccagggcg gcccccagca
      661 agcccaggcc gacggtggcc atgcccacca caccgccggt gcggaaggcg atccgcatcg
      721 ccttctcccg gccgccggac tcgttggacg ccgccgccac ccggatgttg gcgcgggtgg
      781 acagccacat gcccaggtag ccgatcgacg ccgagaatac ggccccgatc aggaagaaga
      841 tcgagcgacc gatccgctcg ctgaggtcct cggcgggaag cgcgaacaac agcaggaaaa
      901 cgatgacggc gaacacggac agcgtcttga actgccggtt caggtaagcc gaggcgccct
      961 cctggattgc ccggccgatc tcctgcatgc gcgacgtgcc ctgaccggct gccaacacct
     1021 cccggagcag tacggcgccg acggccagag cggcgagcgc gacggcagcg accacaccga
     1081 cgacggtgta gtcatccacg ctcagttcga gacccccgcc ctgggcgagg atgaactcgg
     1141 acatccgtcc tcctgattat ccgtgcatcg acaatgcgag catcggcacc gggctgcgcc
     1201 gtcgatggga cggcgttacg gccagtgagt ctaggcgttg ccgtcgcagg tcacgtcgcc
     1261 ggtgccgttg cagcgcccct cacgtctcga actcagctcc ccaccgccag ctcccagctt
     1321 ccccagcggc agggcagctt ggtaaaggtg aaggcgttag cgtgcagtcg agacatacca
     1381 gcgaatggga gccgacggtg cagcgcgggg acgggttcgg ccaggccctg ttgcagggtc
     1441 tgctttgcgg ctcccagggc agtgactcgc cgctgcggca tgtcgccacg attcccgctc
     1501 gggcggcgac gacggtgcag tggccaggct gggtacccga ccagctgcgc gaagccctcg
     1561 gggtggcagg tgtggcgcag ccgtgggcgc accaggtcgc cgttgccgag ctggcccgag
     1621 cggaccgcca cgtcgtggtc gccaccggta ccgcgtcggg taagtcgttg gcctaccagc
     1681 tgccggtact caccggcctg ctgaccgacc agcaggccac cgcgctgtat ctcgctccca
     1741 ccaaggcatt gggtgccgac cagatccgag cggtgaccgc gctgggtttg gacggggtgc
     1801 ggccagccgg ttacgacggc gacaccccga tggccgagcg ggactgggtg cgctcgcacg
     1861 cccggtgggt gttcaccaac ccggacatgc tgcatcacgg actgctcgcc cggcatgatc
     1921 gctgggcacg tctgctgcgg cgactgcggt ttgtggtgat cgacgagtgc cacgcctacc
     1981 gcggggtgtt cggctcacac gtcgcgctgc tgctgcgccg gttgcgccgg gtcgccgccc
     2041 gatacggcgc gcatccggtg ttcgtgctcg cctcggcgac ggtcgcggac ccgggcgcct
     2101 cggccactcg gctcaccggc ctggacacgg ttgcggtcat cgaggacagc tcgccacgcg
     2161 gtgagcgcac cgtggcgttg tgggagccac cgctgttgcc cgagctgacc ggagagaacg
     2221 gggcaccgtt gcgccgctcg gcagcagccg ag
//
LOCUS       DATADN010001108         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1200821, whole genome shotgun sequence.
ACCESSION   DATADN010001108 DATADN010000000
VERSION     DATADN010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200821"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1338
                     /locus_tag="VHH53_14210"
     CDS             211..1338
                     /locus_tag="VHH53_14210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX2291310.1"
                     /translation="MYTLDQHVPQQPAPVDTRADDLAVLNQMLVTLVESRGSDLHLTV
                     GSPPMIRVDGSLQAIPGYGKLNSADTALLARAGVSDEHWRTFQREQELDFAHSITGVS
                     RFRGNLYVQRNSCGAVFRAIPHKIKPLDELGMPESVARFAHLPRGLVLVTGPTGSGKT
                     TTLASVLDLANRSRAAHIITIEDPIEFLHPHKRSVVNQREVGSDTGTFATALKHALRQ
                     DPDIILVGELRDLETTATALTAAETGHLVLATLHTQSATQTIDRVIDIFPPHQQLQIR
                     AQLAASLQGVVTQALAPRADGKGRAVVAEILTATPAIRSLIREGKSHQIPSFMQAGGS
                     EGMLAFDQHLAERVREGLISMPTALEICHSQEELKRLIGRM"
     gene            1341..2573
                     /locus_tag="VHH53_14215"
     CDS             1341..2573
                     /locus_tag="VHH53_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2291311.1"
                     /translation="MPATKTFHYNSIDSTGRKSKGTVEAANESAAMHMLKQRGEVPLE
                     LTAAGQGLNRQLTIPGLGNRTKLKDLAVFARQFATMTASGMSLLRSLAILEEQTSAAA
                     LKKAIGEVRTDVAGGVSLSASMAKHDKVFPRLMIAMIRAGEAGGMIDRALEQIADSLE
                     KDTALRGKIKSALTYPAIVLAFTFVLIAAVLIFIVPIFEQMFKNLGGELPAITQFLVT
                     ASHNMFWLGPLLIAIAVTLTVLYKRRMRESAEFRLTVDRAKLRMPVFGSLFRKLAMSR
                     FSRNLGLLLNVGVPVMQALSVVGETTGNEVINAAMKDVQSAVRDGQPMSTALRQHKIF
                     PEMVTQMIEVGEESGQISQMLDKVADFYDREVDSAAESLTASNEPIMVLVMGAVVGGM
                     VICLYLPMFTIYQNIQGG"
BASE COUNT          465 a          962 c          875 g          383 t
ORIGIN      
        1 cggcgcgcct cgtccaacga gatccgcgag gtcgcgctcg accagggcat gtacgacctg
       61 cgctcggacg gcctcgcgaa ggcggccggc ggcctcacgt cggtccgcga ggtcctgcgg
      121 gtcgccatct gacccaccgc ccctaaaccc gcggcacacg gtgccgaaag gagggccgtc
      181 agcatcagcg agagattgtc ggtgcaccgc atgtacacgc tcgaccagca cgtgccgcaa
      241 cagccggcgc cggtggacac ccgcgcggac gacctggccg tgctcaacca gatgctggtc
      301 acgctggtcg agtcccgcgg ttccgacctc cacctgacgg tgggctcccc gccgatgatc
      361 cgggtcgacg gctcgctgca ggccatcccc gggtacggca agctgaactc cgccgacacc
      421 gcgctgctgg cccgcgccgg cgtcagcgac gagcattggc ggacgttcca gcgcgagcag
      481 gagctcgact tcgcgcacag catcaccggc gtctcccggt tccgcggcaa cctgtacgtg
      541 cagcgcaact cctgcggcgc ggtgttccgg gccatcccgc acaagatcaa gccgctggac
      601 gagctcggca tgcccgagtc ggtggcccgc ttcgcccacc tcccccgggg cctggtgctg
      661 gtcaccggcc cgaccggctc cggcaagacc accacgctgg cgtccgtgct ggacctggcg
      721 aaccgcagcc gggccgcgca catcatcacc atcgaggacc cgatcgagtt cctgcacccg
      781 cacaagcgca gcgtggtcaa ccagcgcgag gtcggctcgg acaccggcac cttcgccacc
      841 gcgctcaagc acgcgctgcg gcaggacccg gacatcatcc tggtcggcga gctgcgcgac
      901 ctggagacca cggcgaccgc gctgaccgcc gcggagaccg gtcacctggt gctggccacc
      961 ctgcacacgc agagcgccac gcagaccatc gaccgcgtca tcgacatctt cccgccgcac
     1021 cagcagctgc agatccgcgc ccagctggcg gcgagcctgc agggcgtggt cacccaggcg
     1081 ctggcgcccc gcgccgacgg caagggccgc gcggtggtcg ccgagatcct gaccgcgacg
     1141 ccggccatcc ggagcctgat ccgcgagggc aagtcgcacc agatcccgtc gttcatgcag
     1201 gccggtggca gcgagggcat gttggccttc gaccagcacc tggccgagcg ggtgcgcgag
     1261 ggcctgatca gcatgccgac cgcgctggag atctgccact cgcaggaaga gctcaagcgc
     1321 ctcatcggac ggatgtgacc atgccggcga cgaagacctt ccactacaac agcatcgact
     1381 cgaccggccg caagtcgaag ggcaccgtcg aggcggcgaa cgagtccgcc gcgatgcaca
     1441 tgctcaagca gcgcggcgag gtcccactcg agctcaccgc ggccggccag ggcctgaacc
     1501 ggcagctgac catcccgggt ctgggcaacc gcaccaagct caaggacctg gcggtgttcg
     1561 cccggcagtt cgccacgatg accgcgtccg gcatgtcgct gctgcggtcg ctggccatcc
     1621 tcgaggagca gacctcggcc gccgcgctga agaaggcgat cggcgaggta cgcaccgacg
     1681 tggccggcgg tgtctcgctg tcggcgtcga tggccaagca cgacaaggtc ttcccgcggc
     1741 tgatgatcgc gatgatccgg gccggcgagg cgggcggcat gatcgaccgg gcgctggaac
     1801 agatcgcgga cagcctggag aaggacaccg cgctgcgcgg caagatcaag agcgcgctga
     1861 cgtacccggc gatcgtgctg gctttcacct tcgtgctgat cgccgcggta ctgatcttca
     1921 tcgtgccgat cttcgaacag atgttcaaga acctcggcgg cgagctgccg gcgatcaccc
     1981 agttcctggt caccgccagc cacaacatgt tctggctcgg cccgctgctg atcgccatcg
     2041 cggtcacgct gaccgtgctc tacaagcgcc ggatgcggga gagcgcggag ttccggctca
     2101 ccgtcgaccg ggccaaactg cggatgccgg tgttcgggtc gctgttccgg aagctggcga
     2161 tgagccgctt ctcccgcaac ctgggcctgc tgctgaacgt cggcgtgccg gtgatgcagg
     2221 cgctgtccgt ggtcggcgag accaccggca acgaggtgat caacgcagcc atgaaggacg
     2281 tgcagagcgc cgtccgggac ggccagccca tgtccaccgc actgcgccag cacaagatct
     2341 tccccgagat ggtcacgcag atgatcgaag tcggcgaaga aagcggtcaa atcagtcaga
     2401 tgctcgacaa ggttgccgat ttctatgaca gggaggtcga cagcgccgcc gagtccctga
     2461 ccgcctcgaa cgaaccgatc atggtgctcg tcatgggtgc cgtggtcggc gggatggtga
     2521 tctgtctgta cctaccgatg ttcaccatct accagaacat ccagggcggc tgacgctctg
     2581 gtcggcgacc ggtctccggt cgacgtcggg cccgagcccg caaccgcacc accatccccg
     2641 ccctaccctg acgccactgg aggcaccccc aatgcaggac atcat
//
LOCUS       DATADN010001109         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1500539, whole genome shotgun sequence.
ACCESSION   DATADN010001109 DATADN010000000
VERSION     DATADN010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500539"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1581)
                     /locus_tag="VHH53_14220"
     CDS             complement(<1..>1581)
                     /locus_tag="VHH53_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX2291312.1"
                     /translation="AARGQARSPRRDFGYSHNILHQAELPVLGVCLGHQGLGLVAGAE
                     IRRAPQPRHGHLTTVHHLGDGVFAGMPQGFTAVRYHSLCVADSLPPELEATAWAEDGV
                     IMGLRHRDLPRWGVQFHPESFCTEHGAQLVANFRDLAVASLASLASRQPAALSPSTAS
                     VQRARPRPPAMRVLVRHIDRAVDTEAAFTTLHRASARAFWLDSAYVEAGLSRFSFLGD
                     ADGPDGEVLTFRIGDDSVLVEPTTGIPYLEPGTIFDALDRRTSRQLSGAQDLPFDLNG
                     GYVGYFGYEMKADLGSSSAHRARAPDAMWIRASRFVAVDHAQHQTYVVAVAEPDATAS
                     AMEWICRTVQTLRTLPTAAAEAPPPAVADNARISDWLVRSRSQYLADIAECQRQLRAG
                     ESYEICLTNKLRLPAPEDDLEFYRRLRRLNPAPYSALLRLGELAIFCSSPERFLRIDS
                     EGTVESKPIKGTAPRSTDSELDEKLRTSLQNDDKIRAENLMIVDLLRNDLGRICQIST
                     VVVPKFMVTESYATMHHLV"
BASE COUNT          257 a          497 c          551 g          276 t
ORIGIN      
        1 accaagtgat gcatggtcgc gtacgactcc gtcaccatga attttggtac cacgacggtg
       61 ctgatctggc atatccggcc gaggtcgttg cggagcaggt ccacgatcat gaggttctct
      121 gcgcggatct tgtcgtcgtt ctgcaacgaa gtccgcagct tttcgtccag ctcggaatca
      181 gtgctgcgcg gtgccgttcc cttgatcggc ttgctttcga cggtgccttc gctgtcaatg
      241 cgcaggaacc gctcagggga tgagcagaag atggcaagtt cgcccagtcg cagcagcgcc
      301 gagtacgggg cgggattgag ccgccgcagc cgccggtaga actccagatc gtcctcgggt
      361 gcgggcagcc tgagtttgtt ggtgaggcag atctcgtaac tctcgcctgc gcgcagctga
      421 cgctggcact ccgcgatgtc cgcgaggtac tgcgaccggg accgtaccaa ccagtccgaa
      481 atccgtgcat tgtcggcgac cgccggcggc ggagcctcgg cagccgccgt gggtagtgtc
      541 cgcagcgtct gcactgtacg gcagatccat tccatcgccg aggcggtggc gtcgggttcg
      601 gccacggcga cgacgtacgt ctggtgctgg gcatggtcca ccgcgacgaa cctgctcgcc
      661 cggatccaca tcgcgtccgg tgcccgcgcc cggtgcgcgc tggacgaacc gaggtcggct
      721 ttcatctcgt agccgaagta gccgacgtag ccaccgttga gatcgaacgg caggtcttgc
      781 gctccgctca gctgccggct tgtccgccgg tcgagtgcat cgaagatggt gccgggctcc
      841 aggtagggaa taccggtggt gggctccacg agcaccgagt cgtcgccgat ccggaaggtg
      901 aggacctcgc cgtccgggcc gtcggcgtcc ccgagaaagg agaaccggga caggcccgcc
      961 tcgacatatg cgctgtccag ccagaacgcc cgggcgctcg ccctgtgcaa ggtggtgaat
     1021 gctgcttcgg tatcgactgc ccggtcgatg tgccgcacga gcacccgcat tgccgggggt
     1081 cggggtcgtg ctcgttgcac cgacgcggtg gatggtgaca gcgcggccgg ctggcgcgag
     1141 gccagggagg ccagggaggc cacggcaagg tcgcggaaat tggccactag ctgggcaccg
     1201 tgctcggtgc agaaggactc gggatgaaat tgcacgcccc accgcggcag atcccggtgg
     1261 cgcaggccca tgatcacacc gtcctccgcc catgccgtcg cttccagctc gggcggcagt
     1321 gagtcggcga cgcacaatga gtgatagcgg accgcggtga acccctgcgg cattccggcg
     1381 aaaacaccgt cgccaaggtg gtgcaccgtg gtcaagtggc cgtgtctggg ttgcggtgca
     1441 cggcgtatct cggcgccggc gaccaggccg agcccttggt ggccgagaca gaccccgagg
     1501 accgggagtt cagcttgatg caagatgttg tgcgagtagc cgaagtctcg gcgcggtgat
     1561 ctcgcctggc cccgggcggc c
//
LOCUS       DATADN010001110         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1050240, whole genome shotgun sequence.
ACCESSION   DATADN010001110 DATADN010000000
VERSION     DATADN010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050240"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /gene="asnB"
                     /locus_tag="VHH53_14225"
     CDS             <1..1501
                     /gene="asnB"
                     /locus_tag="VHH53_14225"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX2291313.1"
                     /translation="VDAGKCERMLNRVQHRGPDDTGILTLDSAWLGHQRLSIVDVSRG
                     RQPLANGDGTAWIIGNGEIYNHEDLSAKLPPGRLQTRSDTEAALHLVMRDGPASVAEL
                     NGMFALAMVTATGNGLVARDPMGVKPLYWIDGADGTMFASELRAFDPEDRPRVASFPP
                     GCLWTPAAGVVRYADSVPVEVRPARRAQHPIWDSGTLEKVRDSLVLSVRRRMMSDVGV
                     GVFLSGGLDSAIVAAIAAEHTRRRGDVLPTFAVGTKDSNDLLAARRVAEFIGSDHHEV
                     VATAESIGEELDNAVEVIEHYDPALVRSAVPNLLLAREAAKKVKVVLTGEGADELYGG
                     YSYVHSPQFADPDALQAELVRSLEQLHHLNLQRCDRTTMYFGLEAREPFLDTNVVRTA
                     LTLPPEWKKTTDGRPEKALLREAFTGWLPDELLWRGKEQFGDGSGAGEVLAALAKAKA
                     KASDTKGAATQPPDSWALRSDEEILYYQIWQRYFSGIRPNSTLGCFATP"
     gene            complement(1542..2393)
                     /locus_tag="VHH53_14230"
     CDS             complement(1542..2393)
                     /locus_tag="VHH53_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX2291314.1"
                     /translation="MRITLAQIDARPGELEANVAHAEQVIADAVSEGTDLVVFPELSL
                     SGYSIGDLKEDLSIQPEDERIAKLVKEAKGAGVLLGFPEAQPHGLHIYNSAAYYENGL
                     LVHVHRKLFLPNYAIFEERKHFLPGQSSRAFPILGGRHRAATLVCNDAWQPQLAYVAA
                     QDGAIILLVPACSAQSMFPERYDSRSYWRGITRFYGRMFQLYVVFVNRVGTEDALRFW
                     GGSHIVDPWGQVIAEADEDRENLLTIDVDLSLVRRRRREIPLVREGRLGLLRREIDRL
                     LEEGGDL"
BASE COUNT          473 a          871 c          881 g          416 t
ORIGIN      
        1 ggttgacgct ggcaagtgcg agcggatgct caaccgggta cagcatcggg gaccggatga
       61 caccgggata ctgacgctcg actcggcctg gttaggccac cagagactct ccatcgtcga
      121 cgtcagccgc ggcaggcagc cgctggctaa cggtgatggc acggcatgga tcatcggcaa
      181 cggtgagatc tacaaccacg aggacctcag cgcgaagctg cctcccggaa ggctacagac
      241 ccggtcggac accgaggcgg cgctgcacct cgtgatgcgg gatggtccgg cctcagtggc
      301 cgagctcaac ggcatgttcg cgttggcgat ggttaccgcc accgggaacg gattggtcgc
      361 ccgcgatccg atgggtgtca agccgctgta ctggatcgac ggggcggatg gcacgatgtt
      421 cgccagcgag ctgcgggcct tcgaccccga ggatcggccc cgagtggcct ccttcccacc
      481 cggctgcctg tggactccgg ccgctggcgt cgtgcgctat gccgactcgg ttccggtgga
      541 agtccgtcca gcccgccggg cgcagcaccc gatctgggac agtgggacgc tcgagaaggt
      601 cagggactca ctggtgctgt ccgtgcggcg tcggatgatg agcgacgtcg gggtgggagt
      661 gtttctctcc ggcgggctcg actcggcgat cgtcgccgca atcgccgcag aacacacccg
      721 ccgccgcggt gacgtgctgc ccaccttcgc ggtcggcacc aaggacagca acgacctgct
      781 ggcggcccgg cgcgtcgccg agttcatcgg cagcgaccac cacgaggtcg tcgccaccgc
      841 cgagtccatt ggcgaggagt tggacaacgc ggtcgaagtg atcgagcact acgacccggc
      901 gctggtccgc agcgcggtgc cgaacctgct gctggcgcgc gaggcagcca agaaggtcaa
      961 ggtggtgctc accggtgaag gcgccgacga gctctacggc ggctacagct acgtgcacag
     1021 cccgcagttc gcggatccgg acgcattgca agccgagttg gtgcgcagcc tagagcagct
     1081 gcaccacctg aacctgcagc gctgcgaccg gacgacgatg tacttcgggc tcgaggcccg
     1141 cgagccgttc ctggacacca acgtggtgcg cacggcgctg accctgccgc cggagtggaa
     1201 gaagaccacc gatggacggc ccgagaaggc gctgctgcgc gaggccttca ccggctggct
     1261 gcccgacgag ctgctctggc gcggcaagga gcaattcggt gacggctcgg gtgccggcga
     1321 agtgctcgct gcgctagcca aagccaaggc caaggccagt gacaccaaag gcgccgcgac
     1381 gcagccaccg gacagctggg cgctgcgcag tgacgaggag atcctttact accaaatctg
     1441 gcagcgctac ttctccggga tacgccccaa ctcgacgctg ggctgctttg cgaccccgtg
     1501 aatggcgatg tgtgccataa caggcgatcg acgctcacga actataggtc gccaccttct
     1561 tcgaggaggc ggtcgatctc ccggcgcagc aagcccagtc gaccctcccg caccagcggg
     1621 atctcccgcc gccggcggcg caccagcgag aggtcgacgt cgatggtgag caggttttcc
     1681 cggtcctcgt cggcttcggc gatcacttgg ccccacggat cgacgatgtg tgagccaccc
     1741 cagaagcgca gcgcatcctc ggtaccgacg cggttgacga agaccacgta caactggaac
     1801 atccgcccgt agaagcgggt gatgccgcgc cagtacgacc gcgagtcata ccgctcgggg
     1861 aacatgctct gcgcgctgca ggcgggcacc agcaggatta tcgccccgtc ctgcgcggcc
     1921 acgtaggcaa gctgcggctg ccacgcgtcg ttacagacca aggtggccgc gcggtgccgt
     1981 ccacccagga tcgggaaggc ccgggacgac tgaccaggca ggaagtgctt gcgttcttcg
     2041 aaaattgcgt agttgggcag gaacaacttg cgatgcacgt gcaccagcag gccgttctcg
     2101 tagtaggccg cgctgttgta gatgtgcaga ccgtgtggct gcgcctcggg aaacccgagc
     2161 agcacgcccg cgcccttggc ctccttgacc agcttcgcta tccgctcgtc ttccggctgg
     2221 atcgacaggt cctccttgag gtccccgatc gagtagccac tgagcgacag ctcggggaac
     2281 accaccaggt cggtgccctc gctgaccgca tcagcgatca cctgctcggc atgtgcgacg
     2341 ttggcctcaa gctcaccggg ccgggcatcg atctgtgcga gcgtaatacg catcgctacc
     2401 tcccggaatc cggcgacggg ggtgggaaag cgctcaacgt accgagcagc tgtcactcac
     2461 cgtagccggg ggcgggaagg gaagccggtc gtccgtgcct acctcgccgg cggtgcggcg
     2521 ctgctcccaa cccataaact gtgtggccgt cacagccatc tcgtcgccga ccaaccatcg
     2581 tgagtatggc tgatgcacgg cgcgtcgatc agtcgaacca ggattgggag acgtcgctga
     2641 c
//
LOCUS       DATADN010001111         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1331967, whole genome shotgun sequence.
ACCESSION   DATADN010001111 DATADN010000000
VERSION     DATADN010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331967"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VHH53_14235"
     CDS             <1..242
                     /locus_tag="VHH53_14235"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEX2291315.1"
                     /translation="PRPDQDRTFRSYRRPMMTNPDEVADRRLDANAAAGPLAELFTVD
                     LTTAVATCAGCGNSAPLAAHLLYADGPAADGHRRA"
     gene            complement(305..1159)
                     /locus_tag="VHH53_14240"
     CDS             complement(305..1159)
                     /locus_tag="VHH53_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX2291316.1"
                     /translation="MTTAVLITGASSGIGHATAQRLSRHPDLTVYATARRTEAIADLA
                     KAGACILALDVTDEKSMRAAVEAVEADHGAVGALVNNAGYGEYGTIEETSLDRVRRQF
                     ETNVFGLARITQLVLPGMRAAGWGRIINIGSMGGRLVFPAGGYYHASKYAVEALTDAL
                     RFEAAPFGIKVSLIEPGLIRTGFSDTAAHTLAGSAAPAGPYAALNAAVDRQMAGSYQS
                     TLLSAPPGAVAKVIEHAVTTTRPRTRYVVTPAAKALVHTRRLLGARVFDAYLRLQFRV
                     PRDSVRDR"
     gene            complement(1599..2396)
                     /locus_tag="VHH53_14245"
     CDS             complement(1599..2396)
                     /locus_tag="VHH53_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2291317.1"
                     /translation="MDVQPDHGERSYRGTGRLAGKKAVITGGDSGIGRAVAIAFAREG
                     ADVLITCLECEQDDARETARWVFDAGRRAVMVAGDLRDEQHCREVIDRAVQEFEQIDV
                     LVSNAAYQMSQDGGLDGVTTEQFDRVMKTNVYALFWLCKAAVPHMPSGSTIITSSSVQ
                     AFQPSPHLMDYAASKAAIVNFTKALGHDLVERGIRANSVAPGPVWTPLIPATMAEDKL
                     ESFGAQAPMGRAAQPAELAPFYVFLASQESSYMTSEVLGVTGGSPIT"
BASE COUNT          403 a          789 c          857 g          410 t
ORIGIN      
        1 cacccaggcc tgatcaagac cgaacgtttc ggagctaccg gaggcccatg atgaccaatc
       61 ccgacgaggt ggcggaccgg cgactggacg ccaacgcggc cgccggccca ctcgccgagc
      121 tgttcaccgt ggacctgacc acagccgtag ccacctgcgc cggatgcggc aacagcgcgc
      181 cgctggcggc acacctgctt tacgccgacg gccctgccgc cgatggtcat cgccgagcgt
      241 gagccgtgga cagccgtgca ggcggtcatg ttcctggacc acgtgcaccg gactgatgat
      301 cgactcaccg atcgcgcaca gagtcacgcg gaacccggaa ctgcagccgc aggtaggcat
      361 cgaacacgcg ggcgccaagc agccgacggg tgtgcacgag ggccttggcg gcgggggtga
      421 ccacgtaccg cgtgcgcggt ctcgtagtgg tgacggcgtg ttcgatcacc ttggcgaccg
      481 cccccggcgg cgccgacagg agggtggact ggtaggaacc ggccatctgc cggtcgacgg
      541 cggcgttgag cgcggcgtag gggccggccg gagcggctga tccggcgagg gtgtgggcgg
      601 cggtgtcgct gaatccggtg cggatcaggc cgggctcgat gaggctgact ttgatcccga
      661 acggggctgc ctcgaagcgc agggcgtctg tgagggcttc cacagcgtat ttgctggcgt
      721 ggtagtagcc gccggcgggg aagacgagcc gaccgcccat ggagccgatg ttgatgatgc
      781 ggccccaccc ggcggcacgc atgccgggta gtacgagctg tgttatgcgg gccagtccga
      841 atacgttggt ctcgaactgg cggcgcactc ggtccaggct ggtttcctcg atggtgccgt
      901 actcgccgta gccggcgttg ttgaccaggg cgccgacggc gccgtgatcg gcttcgactg
      961 cttcgacggc ggcgcgcatg gacttctcgt cggtgacgtc cagcgcgagg atgcaagcac
     1021 cggccttagc gaggtcggcg atggcctcgg tgcggcgggc ggtggcgtag acggtcaggt
     1081 cggggtggcg ggagagccgt tgggcggtgg cgtggccgat gcccgatgag gctcctgtga
     1141 tcaggactgc cgtggtcatg atggttcctg gtgttggtct gcaagtggtt taggcctgcg
     1201 acgagcagcg ccactccgtg gtgtagccgt cgggctgggg cgtcgggctc ggacagggcg
     1261 gcctccaggc cccgagtgag agcgaggacg agttcggtga actcgaccgc ttcgacgccc
     1321 gcgtaggcgg tgctgacgcg ccggcgagca ggtcgcgcaa ggcagcgttg tacgcatcga
     1381 tcattccttc agcctgcgct cgtaggcaag ttcgactgga cgcgctgtct acaacagact
     1441 ctgatctggt ttgttgacca ggtcagagcc gtggagccgc ctcggggaat cgaaccccgg
     1501 acctgctcat taggaggctg tacccgcgct cctctggtat ctaccagaga caacagttgc
     1561 agctctcccc accgaggtgc agcgtcaagc accgatcctc acgtgatcgg tgacccgccg
     1621 gtcaccccga ggacctccga ggtcatgtag ctcgattcct gggaagcaag gaacacataa
     1681 aacggcgcga gctcggccgg ctgcgccgcc cggcccatcg gcgcctgcgc accgaatgac
     1741 tccagcttgt cctcggccat ggtggcgggg atcagcggcg tccacacggg accgggagca
     1801 accgagttag cgcgaatacc ccgctcgacg aggtcgtgac cgagcgcctt agtgaagttt
     1861 acgatcgcgg ccttggaggc tgcgtagtcc atcaggtgcg gggaaggctg gaaggcctgg
     1921 accgaagaac tggtaatgat ggtggaaccg gacggcatgt gcggcacggc cgctttacac
     1981 agccagaaca gtgcatagac attagtcttc atcacccggt cgaactgctc ggtggtgacg
     2041 ccatcgagcc cgccgtcctg cgacatctgg taggccgcgt tgctgaccag tacgtcgatc
     2101 tgctcaaact cctgcacggc gcggtcgatc acctcgcggc agtgctgctc gtcacggagg
     2161 tcaccggcca ccatgacggc ccgacggccg gcgtcgaaga cccagcgagc cgtctcccgt
     2221 gcgtcgtcct gctcgcactc caggcaggtg atcaacacgt ccgctccctc ccgggcgaac
     2281 gcaatcgcca ccgcacggcc gatgccagag tcgccaccgg tgatgacggc cttcttgccc
     2341 gccagacgcc cggtgccccg gtagctgcgc tcaccgtggt ccggctgcac atccatatca
     2401 ccggtgaggc ccggatactc cacggtccga tcgccgctgc ccggtgctgc gtgctgttc
//
LOCUS       DATADN010001112         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_122025, whole genome shotgun sequence.
ACCESSION   DATADN010001112 DATADN010000000
VERSION     DATADN010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122025"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..397)
                     /locus_tag="VHH53_14250"
     CDS             complement(11..397)
                     /locus_tag="VHH53_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291318.1"
                     /translation="MSLVLDSRAITALGRRDRQTAARITALRLRGLWPPIVPTAVLVD
                     SLTGNRIRDAMANRTVELSLVHSLDEPLARRAAQLRGRARAGSAVDAAVVATAELFEA
                     LVVTGDLHDLARLSQHANGVALLPAR"
     gene            complement(394..645)
                     /locus_tag="VHH53_14255"
     CDS             complement(394..645)
                     /locus_tag="VHH53_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291319.1"
                     /translation="MPRVQIYLPDDLHHAVKELELPISELSQHAVRVELRRRQLAADA
                     DRYLAELSVELGGPPTADELAAADAWIDAATVPPARRPR"
     gene            725..1138
                     /locus_tag="VHH53_14260"
     CDS             725..1138
                     /locus_tag="VHH53_14260"
                     /inference="COORDINATES: protein motif:HMM:NF022216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2291320.1"
                     /translation="MTESLTATFRPPLTALIGVFGLTVCVTPVAFGVPGLQVLYLVPL
                     GVGIWLVRTRTVVDRDTVVAHRVVGSRRIPWSDLHGLRIDDRSRVWAVLHDGEEVGLP
                     AVRARDLPLLAALSGGRLPDPLTAGGDRGAPEEQQ"
     gene            1230..1553
                     /locus_tag="VHH53_14265"
     CDS             1230..1553
                     /locus_tag="VHH53_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX2291321.1"
                     /translation="MAWALLAAAIASEVFATINLRLSEGFSKLLPSIFVLVGYVAAFV
                     LLAQVLQQGLALGVAYAIWAAAGVALVALIAAVLLGDSLTGVQVAGLVAVIGGVVALE
                     AGGAH"
     gene            1713..>1895
                     /locus_tag="VHH53_14270"
     CDS             1713..>1895
                     /locus_tag="VHH53_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2291322.1"
                     /translation="MVDDLLVAVITAGAERFAGELRTRMQGSPTVQSLAELAAGYLRE
                     RRGPAITEYELYLLAAR"
BASE COUNT          267 a          594 c          697 g          337 t
ORIGIN      
        1 ggtcacgctg tcaccgggcg ggtagcaggg caaccccatt ggcgtgttgg gacaggcggg
       61 caaggtcatg tagatcgccg gtgaccacca gcgcctcgaa cagctccgcg gtggccacga
      121 cagcagcatc gaccgccgag cctgcccgtg ctcgcccgcg aagctgtgca gcgcgacgag
      181 cgagcggttc gtccagtgaa tggaccaagg acagttccac cgtgcggttg gccatcgcat
      241 cgcggatccg gtttccggtc agcgagtcga cgagtacggc ggtcggcacg atcggtggcc
      301 acagtccacg cagtcgaagc gcagtgatgc gcgctgccgt ctgccgatcc cggcggccga
      361 gagccgtgat cgcccgcgag tcgaggacca ggctcatctc gggcgtcgtg cgggcggaac
      421 cgtggcggcg tcgatccagg cgtccgcagc cgccagctcg tcagccgtcg gcggtccacc
      481 gagctctacg gacagttcgg ccaggtaccg gtctgcgtca gcggccagct ggcggcggcg
      541 taactccact cgcacggcgt gctggctgag ctcggagatc ggcagctcga gttctttgac
      601 ggcgtgatga agatcgtctg gaagataaat ctgaacccgg ggcatacgcc tactgtacgc
      661 ctacccgaga ggccagacat gccgacctgg ccgagcgccg cgtgggccct gtcactatgt
      721 caccgtgacc gagtcgttga cggcgacctt ccggccgccg ctcaccgccc tgatcggagt
      781 cttcgggctc accgtatgcg tgactccggt ggccttcggt gtgcccggcc tgcaggtgct
      841 gtacctggtg ccgttgggtg tggggatctg gttggtgcgg acccgcacgg tggtcgaccg
      901 cgacaccgtg gtggcgcatc gggtggtggg ctcgcggcgc atcccctggt cggacctgca
      961 tggcttgcgg atcgatgacc ggtcccgggt ctgggccgtc ctgcacgatg gcgaggaagt
     1021 ggggctgccc gcggtgcggg ctcgggacct gccgttgctg gcggccttga gtggaggacg
     1081 gctgccagac ccactcaccg ccggcggcga tcggggtgcg cccgaggagc agcagtagcg
     1141 atgcgcagca gcaccgcgga gtagcccagc agcgcggcgt cctcacatct tgcggcactt
     1201 gttcagattg tcgagtggtg ggaggtctca tggcctgggc gttgttggca gctgcgatcg
     1261 cttcggaggt cttcgcgacg atcaacttgc gactgtcgga gggcttctcg aagctccttc
     1321 cgtcgatctt cgtcctggtc ggctacgtcg ccgcgttcgt gttgcttgcc caggtgctcc
     1381 agcagggcct ggcgctcggc gtcgcctacg ccatttgggc agccgccggt gttgccctgg
     1441 tggctctgat cgctgcggtg ctcctgggcg actcgctgac cggcgttcag gtggcgggcc
     1501 tggttgccgt gatcggtggt gtggtggcgc tggaggccgg aggggcgcac tgaggtggtc
     1561 gacggacgtg ttgagagtgg tcgccgacga cgagagctgt tgatcgaagc ggcgttacgg
     1621 gttgccgagc gagatggcgt ggaggcgatc acgcaccgcg cggtcgcgca ggaagccggc
     1681 gtcacgagca ccgctgggac ctaccatttc cgatggtcga cgacctgctc gtcgctgtga
     1741 tcacggcggg ggccgagcga ttcgccggcg agctgcgaac gcggatgcaa ggctccccga
     1801 ccgtgcagag tctcgccgaa ctggccgcag gctacctgcg tgagcgccgc gggccggcta
     1861 tcaccgagta cgagctgtac ctgctggcag ctcga
//
LOCUS       DATADN010001113         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_880193, whole genome shotgun sequence.
ACCESSION   DATADN010001113 DATADN010000000
VERSION     DATADN010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880193"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VHH53_14275"
     CDS             complement(<1..354)
                     /locus_tag="VHH53_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291323.1"
                     /translation="MDPGAAVELTLEFEVGWTLRAAVRIPHLDRSATAVIEIPVREVA
                     PWEQIRARATAARADWAQIRRDVHPAESATAAPEVEHRLTALDALVARTQDQAKAHHL
                     LLEAETILQGLLNSSG"
     gene            complement(327..1991)
                     /locus_tag="VHH53_14280"
     CDS             complement(327..1991)
                     /locus_tag="VHH53_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX2291324.1"
                     /translation="MSLVAGIDLGTTNCCVAVPADADIPNRDELITQRRLRPVGGALI
                     VANPDLSLTTPSAVWIGPDGTPLIGARAKRKARQPGSPPALFFKRNMGTDQPVTAGHA
                     TLTPFEASVLLLKQLKDNAEATLGMTIDRAVITVPAYFETSAKTQTTQAGAEAGLEVA
                     ETLMEPVAAALAHMHENGYTTDGEGPARRFLVYDLGGGTFDTSVVSWAAQEGFTSRSI
                     GGDRYLGGYDFDQAVVDWVIAQLPAYDLHFEPGDPRDDERRARLLALAESAKHELSRE
                     SETEIISDDLEDRAGQPMTINLSLSRVEFDQLIESHLRGTLDHCAEALATARVDPGEL
                     DEIVLVGGSSRVPLVAELLRERFGLTPRLFHPELVVAVGAALKAAGLATRSSVLELEN
                     LAPDGDVVDIAGRVLLSEISGAEIVVEVGTGTGDALRELVSDGGTFLFVDVPIGTSEF
                     TVRVFADGREVANERLGVRTRPGPAVPDVAGDILAHDFSVELLDRQLCRVAKAGTRIP
                     HRVHQHLVTATRGTTLAVRVYEGWIPIGTCASATCPRRWTPAPQWS"
BASE COUNT          314 a          716 c          764 g          329 t
ORIGIN      
        1 accgctgctg ttgagaagcc cctggaggat ggtctcggct tcgagcagga ggtggtgggc
       61 cttggcctga tcctgggtgc gggccaccaa ggcgtcgaga gcggtgagac ggtgctcgac
      121 ctcgggagcg gccgtggcgg attccgcagg gtgcacgtcg cggcggatct gcgcccagtc
      181 ggcgcgcgcc gctgtggcgc gggcgcggat ctgctcccac ggcgccactt cgcgtaccgg
      241 gatctcgatt acggcggtgg ccgaacggtc caggtgcggg atgcgcaccg cggcgcgaag
      301 ggtccaaccc acctcgaact ccagggtcag ctccactgcg gcgccggggt ccatctcctc
      361 gggcaggtcg cggatgcgca ggtgccgatg ggaatccagc cctcgtacac gcgtacggcg
      421 agcgtggtgc cccgggtcgc ggtcaccagg tgctggtgga cgcgatgggg aatcctggtg
      481 ccggccttgg cgacacggca cagctggcgg tcgagcagtt ccacggagaa gtcgtgggcg
      541 aggatgtcgc ccgccacgtc cggcacagcc gggccgggtc gggtgcgcac gccaaggcgt
      601 tcgttggcga cctcccggcc atcggcgaac acgcgaaccg tgaactcgct cgtgccgatc
      661 gggacgtcga cgaacaggaa cgtgcctccg tcggacacca gctcgcgcaa ggcatcaccg
      721 gtgcccgtcc ccacctccac gacgatctcg gcaccgctga tctcggacag cagcacccgc
      781 cccgcgatgt cgacgacatc cccgtcaggc gccaggttct ccagctccag cacgctgctg
      841 cgggtggcca gccccgcggc cttcaacgcc gcacccacag ccaccaccag ctccggatgg
      901 aacaaccgcg gggtgagccc gaaccgctcg cgcaacagct cggcgacgag gggtacccgg
      961 gacgagccgc cgaccagcac gatctcgtcg agttcgcctg gatcgacccg cgccgtggcg
     1021 agcgcttccg cgcagtggtc gagggtgccg cgcaggtggc tctcgatgag ctgatcgaac
     1081 tcgacccggc tcagcgacag gttgatggtc atcggctgcc cggcgcgatc ctcgaggtca
     1141 tcgctgatga tctcggtctc ggactcccgc gacagttcgt gcttcgcgga ttcggcgagc
     1201 gccaacaggc gggccctgcg ctcgtcgtca cgcggatcgc cgggctcgaa gtgcaggtcg
     1261 tacgcgggca gctgcgcgat cacccaatcg acgacggcct ggtcgaagtc gtagccaccg
     1321 agataccggt cgccaccgat ggaccggctg gtgaagccct cctgcgcggc ccacgagacc
     1381 acggaggtgt cgaaggtgcc gccgcccagg tcgtagacga ggaagcgccg ggcgggccct
     1441 tcgccatccg tggtgtatcc gttctcgtgc atgtgggcca gcgcggcggc caccggttcc
     1501 atgagcgtct cggccacctc cagcccggct tccgcacccg cctgcgtggt ctgggtcttg
     1561 gcgctggtct cgaagtaggc gggcacggtg atgacggccc gatcgatcgt catgcccagg
     1621 gtcgcttccg cgttgtcctt gagctgtttc agcagcagca ccgaggcttc gaacggggtg
     1681 agtgtggcgt gtcctgcggt cacgggctga tcggtgccca tgttgcgttt gaagaacagg
     1741 gcgggcggcg aacccggctg gcgggccttg cgtttggcac gtgcgccgat gaggggggtg
     1801 ccgtcgggcc cgatccacac cgcggacgga gtggtgagcg agagatccgg attggcgacg
     1861 atgagcgccc cgccgaccgg gcgcagccgc cgctgcgtga tcagctcgtc acggttcggg
     1921 atgtcggcgt cggcaggcac ggccacgcag cagttggtgg tgccgagatc gataccggcg
     1981 acgaggctca tgcggtggtg gtcccctcgg tcgggtgctg gatctgtccg ggctcgggaa
     2041 gaagctcggg caggacgctc ggatcgagcc acgacgccat gccctcctga acaccgtcct
     2101 cctggacgcc gccgacgccc gca
//
LOCUS       DATADN010001114         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_447793, whole genome shotgun sequence.
ACCESSION   DATADN010001114 DATADN010000000
VERSION     DATADN010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447793"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VHH53_14285"
     CDS             <1..512
                     /locus_tag="VHH53_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291325.1"
                     /translation="LVGVVLAVLVVAGAGTLATAGSSGERGTPSANQKATSVPAAPPV
                     NPLGVLPAPPQVQPVDHSQGSIAWRDAGQLVIDYYNGLNNLPAAWQMLSPTARAAFDN
                     EAGFRSHWSRYSSVSARNASGVTDNPDGSVTVPVDVVYDAGSGAQVVKRQLRVAQRDG
                     QLLIDSDPR"
     gene            complement(513..1130)
                     /locus_tag="VHH53_14290"
     CDS             complement(513..1130)
                     /locus_tag="VHH53_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX2291326.1"
                     /translation="MQRALGDWGTFLAAAIRSPREVGTLLPSGPELARRLAAVVPRVS
                     PPGRPAVVVEVGAGAGAVTWAIAEQVDQDAVVIAVEKDAGLAEGLRSRQLGVQVVTAD
                     AATLPAILAEHGVDRADVIISVLPWTLFGPQQQRELMAVFAAALHTDGVFTAAAYSLG
                     YWTSAARRFRCELERTFGEVLPTRTMWRHLPPAMTYVCRRPGGVP"
     gene            1268..>1599
                     /locus_tag="VHH53_14295"
     CDS             1268..>1599
                     /locus_tag="VHH53_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2291327.1"
                     /translation="MAPLRDDRYLDALIVQSALFAEALRGADLKQPVPTCPDWTLYQL
                     TEHIGQAHRWATTIVIRRATAPPCPTELVDVLGRCGQIQLGGTGRGGGASDDDRGGPP
                     MGLPDVLS"
BASE COUNT          259 a          546 c          550 g          244 t
ORIGIN      
        1 tgctcgtcgg ggttgtcctc gcggtcctcg tggtggccgg tgccgggacg ttggcgactg
       61 ccggttcatc cggagagcgg ggcactcctt ccgccaatca gaaggccacc tcggtgccgg
      121 cagcgccgcc ggtgaaccca cttggtgtcc tgcctgcgcc accacaagtc cagccggtgg
      181 accactctca gggatcgatt gcgtggcgcg atgcgggcca gttagtgatc gactactaca
      241 acggtctgaa taacctgccg gcggcctggc agatgctgtc ccccaccgca agagcggcat
      301 tcgacaatga ggcgggcttc cggtcgcatt ggagccggta ctcctcggtc agcgctcgca
      361 acgcatccgg cgtcacggac aacccggacg gttcggtgac ggtgcccgtt gatgtcgtct
      421 acgacgccgg cagcggagcc caggtcgtga agcggcagct gcgggtggcg caacgagacg
      481 gtcagctgct gatcgactcg gacccgcgct gatcacggga ccccgccggg tcgccggcag
      541 acataggtca tcgccggcgg taggtgccgc cacatggtgc gggtgggcag cacctcaccg
      601 aaggtccgct ccagctcgca ccgaaatcgg cgggctgcgg aggtccagta acccaagctg
      661 tatgccgcgg cggtgaacac cccgtcggtg tgcagggcag cggcgaagac agccatgagc
      721 tcgcgctgct gctgcgggcc gaacagagtc cacggcagca cggagatgat cacgtcggcg
      781 cggtccacgc cgtgttcagc gaggatggcc gggagggtgg cggcgtccgc cgtgacgacc
      841 tgcacaccga gttggcgcga ccgtaaaccc tcggcaagcc cggcgtcctt ctcgaccgca
      901 atcaccaccg catcctgatc cacctgttcg gcgatcgccc aggtcaccgc gccggcaccg
      961 gctccgacct ccaccacgac cgcgggtcgc ccgggcgggc tcactcgcgg tacgacggcg
     1021 gccagccgcc gggctagctc ggggccggac gggagcagtg tcccgacttc gcggggagat
     1081 cgaatcgccg ccgcgagaaa tgtgccccag tcgccgagcg cacgctgcac ggtgtcttgc
     1141 ggttgcgggg caggatgtga ggccataggc cacagtgcac accacccggt gcgccaccgc
     1201 attaccaacc agcagctgct taggctggag tccccgatga ccacggggac caccacggga
     1261 ggcactgatg gctccgctgc gcgatgatcg atatctcgac gcgttgattg tgcaatcggc
     1321 gctgttcgcc gaggcgctgc gcggagccga cctgaagcaa ccggtaccga cctgcccgga
     1381 ctggacgctg taccagctca ctgagcacat cgggcaggcc catcggtggg ccaccacgat
     1441 cgtcatccgg cgcgccaccg ccccgccctg tcccaccgag ctcgtcgatg tcctcgggcg
     1501 ctgcggtcag attcagctcg gtgggacagg gcggggcggt ggcgcgtcgg atgacgatcg
     1561 tggtggccca ccgatgggcc tgcccgatgt gctcagtga
//
LOCUS       DATADN010001115         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1050437, whole genome shotgun sequence.
ACCESSION   DATADN010001115 DATADN010000000
VERSION     DATADN010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050437"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..270)
                     /locus_tag="VHH53_14300"
                     /pseudo
     CDS             complement(172..270)
                     /locus_tag="VHH53_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025883.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4439 domain-containing protein"
     gene            complement(368..895)
                     /locus_tag="VHH53_14305"
     CDS             complement(368..895)
                     /locus_tag="VHH53_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291328.1"
                     /translation="MSGPRHSRRRMLGAGAALLVAGPTVLAGCTGSTPAAPAEPDPLE
                     QPARRAETDSVLAQAVAQTAAEIANQADGQALTTAATALATDRMAHAIALRTELRRVR
                     PATVPRSIAPSAPPIPAPPPAVTDLTSARAALLQAVHSAQDEASQLVATLPGYRAAML
                     ASVAACCASHAAVLL"
     gene            1072..1599
                     /gene="rimP"
                     /locus_tag="VHH53_14310"
     CDS             1072..1599
                     /gene="rimP"
                     /locus_tag="VHH53_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX2291329.1"
                     /translation="MVTTEPANSDSAVESVVRDAVVGTGFELEDLEEVRAGQRWLVRV
                     IIDSDAGVGLDDIATVSRAVSQALDGCDELLGGPYTLEVTSPGVDRPLTQPRHWRRNR
                     LRLVRVVPAEGAELVGRIGDCDDEGVTLLVSGALRRVRYAQLRRAVVEVEFRPPPAAE
                     LAALDGARRQDLEER"
     gene            1596..>2227
                     /locus_tag="VHH53_14315"
     CDS             1596..>2227
                     /locus_tag="VHH53_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX2291330.1"
                     /translation="MNVDIAALRAIERDKDISFETVLEAIETALLTAYKHTDGHQPHV
                     RIDVDRKTGLVRVLAQELNDDGSVRHEWDDTPEGFGRIAATTARQVILQRLRDAEHER
                     TFGEFSAKEGEIVAGVVQRDARANARGVVVVKIGDIEGVIPPVEQAPRETYRHGERIK
                     CYVVGVSRGLRGPQITLSRTHPNLVRRLFALEVPEIADGTVEIPAVAREAG"
BASE COUNT          354 a          717 c          816 g          340 t
ORIGIN      
        1 aggctgcgtt ggcggccgag catgcggcgg tctgggtgta cggggtggcg ggggcgttcc
       61 tggcggatga gttgtccggt cagctccatg aggccgcagg cgcgcaccag gctcggcgcg
      121 atgccaccga gcctggtgcg cgcctgcggc ctcgtggagc tgaccggaca actcatccgc
      181 caggaacgcc cccgcaaccc cgtacaccca gaccgccgca tgctcggccg ccaacgcagc
      241 ctggagcgct tccaccggca cgtccctcat gatcgatcgg ccccatctgg agacatatcg
      301 gggtcagaag gggcctgaac accccgatat gtctccatat cggggaatgc gagaacagag
      361 cgagcgttca tagcaggact gcggcgtggc ttgcgcagca ggcggcaacc gacgccagca
      421 ttgctgcgcg gtagccgggc agtgtcgcca ccagttggga tgcctcgtcc tgggccgagt
      481 gcaccgcctg aagcagagcg gcgcgagcgc tggtcagatc agtaacggcc ggtgggggcg
      541 caggtatggg cggggctgac ggggcgatgc tgcggggaac cgtcgccggc cggactcggc
      601 gcagctcggt gcgaagtgcg atcgcatgcg ccattcgatc tgtggcgagt gccgtcgccg
      661 ccgtagtcaa ggcctgacca tccgcctggt ttgcgatctc ggctgccgtc tgggccactg
      721 cctgcgccag gaccgagtca gtctcggcgc gccgggctgg ttgctccagc gggtccggct
      781 ccgcgggcgc cgctggtgtt gatccggtgc acccggccag cacggtcgga ccggcgacga
      841 gcagcgcggc cccggcgccc aacatgcggc gtcgactgtg gcgaggtccg gacacgattg
      901 gccatcctgc cagccccccg atccgccggt gcgcagcgcc gtgatccatt tggcgcagat
      961 tgttcccgcg acgcgatacc ctgggtgatc ccgaccgggg cgcgcccccg cgctggtcgt
     1021 cgcccgcatc gccacatcga cagacagaca gttactttca ggcaggaggc catggtgacc
     1081 actgaacccg cgaactccga ctcagcggtc gagtcggtgg tgcgcgacgc ggtggtcggc
     1141 accgggttcg agcttgagga tctggaggag gtacgcgcgg gccagcgctg gctggttcgc
     1201 gtgatcatcg attcagacgc gggcgtcggg ctggacgaca tcgccacggt cagccgtgcg
     1261 gtgtcccagg cgctggacgg ctgcgatgag ctgctcgggg ggccttacac ccttgaggtg
     1321 acctcgccag gtgtggatcg gccactgacc cagccgcggc actggcggcg taaccggctg
     1381 cggctggtcc gggtcgtgcc ggcagagggt gcggagctgg tcggtcggat cggggactgc
     1441 gacgacgagg gcgtcacgct gctggtgtca ggagcccttc gccgggtgcg ctatgcgcag
     1501 ctgcgccgcg cggtggtgga agtggaattc cgcccgccgc ccgccgccga gctcgctgcg
     1561 ctggacggcg cgagacggca ggatttggag gaacggtgaa cgtcgacatc gccgcgctgc
     1621 gcgccatcga acgggacaag gacatctcct tcgagacggt gctcgaagcc attgagacag
     1681 cattgctcac cgcgtacaag cacaccgacg gtcaccaacc gcacgtacgg atcgatgtcg
     1741 accgtaagac cggcctggtc cgggtgctgg cgcaggagct caatgacgac gggagtgtcc
     1801 gccacgagtg ggatgacacc cccgagggat tcggccggat cgcagctacc acggcccgcc
     1861 aggtgatcct gcagcggttg cgcgacgccg agcacgaacg caccttcggc gagttctcag
     1921 ccaaggaggg tgagatcgtc gccggcgtcg tgcagcgcga cgccagggcg aacgcccggg
     1981 gtgtggtagt ggtcaagatc ggcgatatcg agggcgtgat cccaccggtg gagcaggcgc
     2041 cgagggagac ctaccggcac ggcgagcgga tcaagtgcta cgtggtaggc gtgtcccggg
     2101 gattacgcgg gccgcaaatt acgttatccc gtacgcatcc caacctggtc cgccggttgt
     2161 tcgcgctgga agttccagag atcgccgacg gcaccgtgga gatccccgcg gtggccaggg
     2221 aagccgg
//
LOCUS       DATADN010001116         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_447918, whole genome shotgun sequence.
ACCESSION   DATADN010001116 DATADN010000000
VERSION     DATADN010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447918"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHH53_14320"
     CDS             complement(<1..224)
                     /locus_tag="VHH53_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291331.1"
                     /translation="MGTMAVPCDDHPQWCVLTRDHHPYGPRDIDHQSADLTIRLTDFS
                     LTGKLVQIDEIGVGRGEPEVIVEAHDEVESG"
     gene            complement(768..1007)
                     /locus_tag="VHH53_14325"
     CDS             complement(768..1007)
                     /locus_tag="VHH53_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291332.1"
                     /translation="MNEMDEAVSRAIAAALPSQEEVLRAIREGITFGMPPGDVFGEAV
                     SYAMPRQSEILRAIREGVETAVRAYLDANGLDMPW"
     gene            1170..1883
                     /locus_tag="VHH53_14330"
     CDS             1170..1883
                     /locus_tag="VHH53_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEX2291333.1"
                     /translation="MTVGAPSYKGFRFPAQIISHCVWLYHRFPLSFREIEEMMLQRGI
                     TVSHETVRQWCAKFGQTYANGLRGRRARPGDKWHVDEVFIKIRGKTHYLWRAVDQQGT
                     VLDILVTSRRDTRAATRFFRKLLTGLEYVPRVLVTDKLGSYAAARRRVLRSAEHRQSK
                     YLNNRAENSHQPTRQRERAMKTFTSARHAQQFLSAFSGISPHFRPRRHRLGVEDYRRE
                     MADRFTTWTEVTGTATTAA"
BASE COUNT          326 a          681 c          657 g          375 t
ORIGIN      
        1 ccagactcca cctcgtcatg cgcctcgacg attacctcgg gctcacccct acccactccg
       61 atctcgtcga tctgcaccaa cttgccggtc agactgaagt cggtgagccg gatcgtgagg
      121 tccgcggact ggtggtcgat gtcgcgtggt ccgtaggggt gatggtcccg ggtcaacacg
      181 caccattgcg ggtggtcgtc acaggggact gccatagtgc ccatggttct gccttcctgc
      241 tttgctcagg ttggtgcgac aaggcctcgg tatggtgttc accggcacct gccggggccg
      301 ccagacaacc ttttatgtcg tatcacgccg tttgtcgtta tcacaccgtt gaacgttgca
      361 gtagtcggca accgtgcggg gtagacgggg tccacgtggg ttggtagccg ccgctgtcaa
      421 cacgtagggc accccctgat cacacacgcg caccgagccg cctgcccctg cgttaattcc
      481 ggggtctcaa actcgccggc accttcacgg ccgcaccggg cgcatacccc gatgcccggg
      541 cctctgcacc cggacgcgcc gccgctacgc ggtcgcttcg cgctccttga caggccgcac
      601 ccgacgcaga gcgggctatg gatcgggtga agatgcgaag gcccaccagc acgggagacc
      661 agtgggcctt gcgccttact cagctgggag aggttaacgg gctagttcgt ctacgaggtc
      721 tgcggcccat accaggatcg ttgtcaggca acgtggttct gcgggcgcta ccacggcatg
      781 tccagtccgt tggcgtcgag gtaggcccgt acggccgtct cgactccctc gcggattgcg
      841 cggaggatct ccgactgacg cggcatcgcg tacgagaccg cctctccgaa tacgtcgccg
      901 ggcggcatcc cgaatgttat gccctcgcgg attgcgcgga ggacttcctc ttgacttggt
      961 agtgccgctg cgatggccct gctgacagcc tcgtccattt cattcacggt cgggtgcctc
     1021 ctgtggcccg ctgtcgacgt acgccatgag cgccttgcgg cttcccgctg cgggatccac
     1081 cagggaaccc actccggttg acactgtcaa cttggtgatg tggggggttg ggatgatcga
     1141 cgggttgttt ccggggatga ggaggttggg tgaccgtcgg tgcgccgtcc tacaaggggt
     1201 ttcggtttcc ggcgcagatc atcagccact gcgtctggct gtaccaccgc ttcccgctga
     1261 gcttccgcga gatcgaggag atgatgctgc agcgcgggat caccgtcagc cacgagacgg
     1321 tccggcagtg gtgtgccaag ttcggccaga cctacgccaa cggcttgcgt gggcgccggg
     1381 ctcgcccggg ggacaagtgg catgtcgacg aggtgttcat caagatccgc gggaagaccc
     1441 actacctctg gcgggcggtg gaccagcagg gcaccgtgct ggacattctg gtcacctccc
     1501 ggcgcgacac cagggccgcg accaggtttt tccgtaagct gctcacggga ctggagtacg
     1561 tgccccgggt gctggtgacc gacaagctgg gcagctatgc cgcggcgcgc cgccgggtgc
     1621 tgcgctcggc cgagcaccgt cagtcgaagt atctgaacaa cagggccgag aactcccacc
     1681 agcccacccg gcagcgggaa cgagcgatga agacgttcac ctctgctcgg cacgcccagc
     1741 agttcctgtc cgccttcagt ggcatctccc cgcacttccg gccccgtcgt caccggcttg
     1801 gcgtcgagga ctaccgccgc gagatggccg accggttcac cacctggacc gaggtcacgg
     1861 gcacggccac cacggccgcc tgacacagac cagagatcac ccaagccgcc cgaccctgcc
     1921 tccacaacca agcaggctca ccaagttgac agtgcccgct gcgcgcggcc tgtcggccgt
     1981 gctcgcgcgg gcgggctcgg cgggcgcttg tgtcgagaag catgctgccc agtgtgcgc
//
LOCUS       DATADN010001117         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_279174, whole genome shotgun sequence.
ACCESSION   DATADN010001117 DATADN010000000
VERSION     DATADN010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279174"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VHH53_14335"
     CDS             <1..693
                     /locus_tag="VHH53_14335"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX2291334.1"
                     /translation="RLSALVAGGSRDELLSQVEAASLARQVSGNAAEYGGNAIAEADR
                     AAAEAAQAQERAAAAEAEVAAGAGEAERRKGDLDRQIAEVRSALDRLTPEQRSLLSTS
                     EFDTADVKIPSGDVGAVLRFALQQVGKPYIWGAVGPAAYDCSGLVQTSFRVGGVLMPR
                     VSIDQSGVGQQVLRHDVQAGDLIFYYNPVHHVAIAVDNLRAVHAPSFGETVKIAGIDR
                     IGPITVIRRVMH"
     gene            846..1514
                     /locus_tag="VHH53_14340"
     CDS             846..1514
                     /locus_tag="VHH53_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX2291335.1"
                     /translation="MGLTRGSTAGLVPTIAAIGTLIAPGVAAAQADTQPPSAPTQLRV
                     QDVSFTSATFTWSPSTDASGWVMYEVEASALPRSLQRFGSTEPRKTVTGLVPGLTYTA
                     SVVAVDGAGNRSAPASTQFTTPVDATPPTTPTNLRAVTVDGTLDTIVWNAATDRSALR
                     YVVRSSGNWLYGTRSTSVTTFDLLYIVCVVPPGSTHTLTVEAMDEHLNVSARSNPLTV
                     TFPS"
BASE COUNT          240 a          512 c          566 g          252 t
ORIGIN      
        1 cgcctgtccg cgctggtggc cgggggcagc cgcgacgagc tgctgagcca ggtcgaggcg
       61 gccagcctgg ccaggcaggt gtcgggcaac gccgccgagt acggcgggaa cgcgatcgcc
      121 gaggcggacc gggcggcggc cgaggcggca caggcgcagg agcgggccgc ggccgccgag
      181 gccgaggtgg cggccggggc cggcgaggcc gagcggcgca agggcgatct ggaccggcag
      241 atcgcggagg tcaggtcggc gctggaccgg ctgacgcccg agcagcggtc gctgctgtcg
      301 accagcgagt tcgacacggc cgatgtcaag atcccctccg gggacgtggg ggcggtgctt
      361 cggttcgcct tgcagcaggt gggaaagccg tacatctggg gcgcggtggg tccggcggcc
      421 tacgactgct ccggcctggt gcagacgtcg tttcgggtcg gcggggtcct gatgcccagg
      481 gtgtcgatcg accagtccgg ggtcgggcag caggtgctgc gccacgacgt gcaggcggga
      541 gatctgatct tctactacaa cccggtacac cacgtggcga tcgcggtgga caacctgcgc
      601 gcggtacacg cgccgagctt cggcgagacc gtcaagatcg ccggcatcga ccggatcggg
      661 ccgatcacgg ttatccggcg ggtcatgcac tgagggtggg ttaggcgacg caggcgtcac
      721 ctccgcggtt ttcgcggatt gccgccgctt ttactttttc cggccgcggt gaacccctgc
      781 gggtcgccgg gcgtagtagt ccacgtgcgg gcatccagat ccccatttga ggagtgtcga
      841 tgccaatggg actcacgcgc ggcagcaccg ccggtctcgt accgacaatt gccgctatag
      901 ggacgttgat cgcaccgggc gtggcggcgg cgcaggctga tacccagccg ccttccgcac
      961 cgacccagct tcgcgtgcag gacgtttcgt tcaccagcgc gacgttcacc tggagccctt
     1021 ccaccgatgc gtccggttgg gtgatgtacg aggtggaggc cagcgcgttg ccgcgctcgc
     1081 tgcaacggtt tggctcgacg gagccgagaa agacggtgac cgggctggtg cccgggctta
     1141 cctacacggc ctccgtcgtg gccgtcgatg gcgcgggcaa ccggtcagct ccggcgtcga
     1201 cccagttcac cacgccggtg gacgccacgc cgccgaccac gccgaccaac ctgcgcgcgg
     1261 tgaccgtcga tgggacactg gacacgatcg tgtggaatgc cgcgacggac cgttcggccc
     1321 ttcgttacgt cgtccggtca agcggcaatt ggctctacgg cacccgcagc acgtcggtga
     1381 ccacgttcga tctgctgtat atcgtgtgcg tcgtcccacc cggcagtacg catacgctga
     1441 ccgtggaggc aatggacgaa caccttaacg tgtccgcccg cagcaacccg ctgaccgtga
     1501 ccttcccgag ctagcaatca gtccattgaa tccatccggc cgtcaaatgg ttgtcggcgg
     1561 ctcggtggcg
//
LOCUS       DATADN010001118         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1500981, whole genome shotgun sequence.
ACCESSION   DATADN010001118 DATADN010000000
VERSION     DATADN010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500981"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VHH53_14345"
     CDS             complement(<1..286)
                     /locus_tag="VHH53_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2291336.1"
                     /translation="MLHPAGVSPPPAPDAAAGRGPSWLDGWLANHGTDLVAWRRHIHA
                     HPELAYHEQGTTALVAEQLASTGLRPTLLPGGTGLICDIGTGSRCVALRAD"
     gene            complement(304..567)
                     /locus_tag="VHH53_14350"
     CDS             complement(304..567)
                     /locus_tag="VHH53_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291337.1"
                     /translation="MSTATATLTSRVIPAKTSALYYLRTPGVESRQQPEIRVRACSEA
                     EAFAIGCAHEVTPVSPACIDFVQVIGGVSRRCMVPVITVRGAV"
     gene            complement(591..2171)
                     /locus_tag="VHH53_14355"
     CDS             complement(591..2171)
                     /locus_tag="VHH53_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2291338.1"
                     /translation="MVDKTSESGFAIKPVYQAEDIADGLADRLGQPGEFPFTRGVYPT
                     MYTQRLWTMRQYAGFGTAAESNARYHQLLQAGQAGLSVAFDLPTQMGYDSDEVIAHGE
                     VGKVGVAIDSIEDMRRLFDGIPLDQVSTSMTINAPASVLLLLYQLVGEENAVPGSKLT
                     GTVQNDVLREYIARGTYIYPPAQSLRLTSDIFAYCKAEIPRWNTISISGYHMAEAGAN
                     PAQEIGFTLANGVQYVQAALDAGMAVDEFAPRLSFFFVSRTTLLEEVAKFRAARRIWA
                     RLMKERFGARNPKSLMLRFHTQTAGVQLTAQQPEVNLIRVTTQAIAAIFGGTQSLHTN
                     AYDEAIALPTEKAARLALRTQQMLALETDLTSTVDPFAGSYAVEAMTDEVEAVAVALI
                     EQVDSFGGAVHAIEAGFQKSEIEQTAYRTAREIDSGDRVVVGLNRYRLDTEERYQPLR
                     VDPMIEDSARARLAALRSSRSSADVAHALDGLRRAAAGSDNVLYPMKSALAARATVGE
                     VCHALRDVWGVHVPPESF"
BASE COUNT          404 a          772 c          755 g          379 t
ORIGIN      
        1 ggtcggcgcg cagggccacg catcgcgagc cggtaccgat gtcacagatc agaccggtgc
       61 ccccgggcag cagcgtgggc cgcagaccgg tactggcgag ctgctcggct accagtgccg
      121 tggtgccctg ctcgtggtag gcaagttccg gatgcgcgtg gatgtgcctg cgccaggcga
      181 ccagatcggt gccgtggttc gccagccaac cgtccaacca ggacgggccc cgaccggccg
      241 cggcgtccgg cgcagggggt ggcgacaccc cggctgggtg caacaaagcg gattccagcg
      301 cagtcacacg gcacctctga cggtgatcac cggtaccatg cagcgccggg acaccccgcc
      361 gatcacttgc acgaagtcga tgcaggcggg gctaaccggg gtcacctcgt gagcgcaccc
      421 gatggcgaac gcctccgcct cggaacaggc ccgtacccgg atctccggct gttgccggga
      481 ctccacgcca ggcgttcgca aatagtacag cgctgatgtc ttcgccggaa tgacacggga
      541 agtgagggtg gctgtcgctg tactcatccc gtaattctgc ccgatggaga tcagaaactt
      601 tctggaggca cgtggacgcc ccacacgtcg cgcagtgcgt ggcacacctc tcccaccgtg
      661 gcgcgagcgg ccagtgcgct cttcatcggg tacagcacgt tgtcgctgcc ggccgcggcc
      721 ctgcgcagcc cgtccagggc gtgagcgacg tctgcggatg atcgcgacga ccgcagtgcg
      781 gcgagccgcg cccgggcact gtcctcgatc atcggatcca cccgcaaagg ttggtagcgc
      841 tcctcggtgt cgagccggta gcgattgagc ccgacgacga cacggtcgcc ggagtcgatc
      901 tccctggcgg tgcggtacgc agtctgctcg atttccgact tctggaagcc cgcctcgatg
      961 gcatgcaccg caccaccgaa cgagtccacc tgctcgatca acgccaccgc gacggcctcg
     1021 acttcatcgg tcatcgcctc gacagcgtaa gagccggcaa agggatccac cgtggacgtc
     1081 aagtcggtct ccaaggccag catctgctgg gtccgcagag ccagccgggc cgccttttca
     1141 gtgggcaggg cgatcgcctc atcataggca ttggtgtgca acgactgggt accgccgaag
     1201 atcgcggcga tcgcctgcgt agtgacccgg atcaggttca cctcaggctg ctgagcggtg
     1261 agctgcaccc ccgcggtctg ggtgtggaac cgcagcatca gcgacttggg gttgcgcgcg
     1321 ccgaagcgct ctttcatcag ccgagcccag atccgccggg cggcgcggaa cttcgcgacc
     1381 tcctccagca gcgtggtacg ggagacgaag aaaaacgaca gccgcggggc gaactcgtcg
     1441 acggccatcc cggcatccag cgcggcctgc acgtactgca ccccgttggc gagcgtgaaa
     1501 ccgatctcct gcgcgggatt cgcgcccgcc tcagccatgt ggtagccgga gatcgagatg
     1561 gtgttccagc gcgggatctc ggccttgcag taggcgaaga tgtccgaggt cagccgcagg
     1621 gattgcgccg gtggatagat ataggtgccc cgggcgatgt actccctgag cacgtcgttc
     1681 tggaccgtcc cggtcagctt cgagcccggc actgcgttct cctcgcccac cagctggtaa
     1741 agcagtagca gcacgctcgc gggcgcattg atggtcatcg aggtggacac ctgatccaac
     1801 gggataccgt cgaacagcct tcgcatgtcc tcgatcgagt cgatggcaac cccgaccttg
     1861 ccgacctcgc cgtgcgcgat gacctcgtcg gagtcgtaac ccatctgcgt cggcaaatcg
     1921 aaggccaccg acagcccggc ctgcccagcc tgcagcagct ggtgataacg ggcgttggat
     1981 tcagcggcgg taccgaatcc ggcgtactgg cgcatcgtcc agagacgctg ggtgtacatg
     2041 gtgggataca ccccgcgggt gaacggaaac tcccccggct gaccaagccg gtccgccagg
     2101 ccatccgcga tgtcctcggc ctggtacacc ggcttgatcg caaagcccga ttctgaagtc
     2161 ttgtcgacca tcaggtctgt atggcacaca ccagcggcaa ccacaacgca gggcatcatg
     2221 tggcggtgac caacccctca ccccgcctgg tggcggggcg ctaccggctc agtgccgtgt
     2281 tgggtcgggg cacgatgggc acggtgtggt
//
LOCUS       DATADN010001119         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1332452, whole genome shotgun sequence.
ACCESSION   DATADN010001119 DATADN010000000
VERSION     DATADN010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332452"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VHH53_14360"
     CDS             <1..813
                     /locus_tag="VHH53_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEX2291339.1"
                     /translation="ASGALIAMTYGHRSRYIGNPLVDRVVPLPDDLDPLLGIYVAHMG
                     PICANGLLHAAAELHGTDVRSLADGVRGRHIVITGAGVVGLLTALFVRRHGAACVVVI
                     DPTEQRRAAATALGFETVDPDFADPAPILKARWRHGPADRGADVVFQCRGQAATLGLA
                     LRLLRPQGTVIDLAFHQAGADALRLGEEFHHNGLTLRSAQIGRVPRGLAHYWDRERLS
                     TETLELLRTDGPAIRKHLVTDVLSLAEAPALLADLDTHRKHVLQAVFTFEQC"
     gene            complement(839..>1531)
                     /locus_tag="VHH53_14365"
     CDS             complement(839..>1531)
                     /locus_tag="VHH53_14365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2291340.1"
                     /translation="TLGLGSGENLNEHVVGLGWPSVAVRHERLVEALDIINALFDGGY
                     VTLRGQHYQVDSAKLWDLPTQRVPIAVAVSGGQSCEAFAPLADHMIAVEPKPELGRMW
                     DAARPDKGSSRRIGQLPICFDADRDAAISRAHEQFRWFAGGWKVNAELPSTAGFADAS
                     QFLRPQDIAESISCGPDVAAHVAAARPFAEAGFTDLALVQIGGAPQHQEPFLDYAADE
                     LLPALRAEFGVG"
BASE COUNT          253 a          537 c          483 g          258 t
ORIGIN      
        1 gccagcgggg cgctgattgc catgacctac gggcatcgca gtcgctatat cggaaatccg
       61 ctggtggacc gggtggtgcc gctgcccgac gatctcgatc cattgctcgg tatctacgtc
      121 gcccatatgg gtcctatctg cgccaatggc ctgcttcacg cagccgctga actgcacggc
      181 acggacgtgc gctcactggc cgacggggtg cgcggccggc acatcgtgat caccggggcc
      241 ggtgtcgtcg ggctgctgac cgcgctgttc gtgcggcggc acggggcagc ctgcgtggtg
      301 gtgatcgacc cgaccgaaca gcgccgggcc gccgctaccg cgctgggctt cgagaccgta
      361 gatccagact ttgccgaccc ggcaccgatc ctgaaggcac gctggcgcca tggacccgcc
      421 gaccgtggcg ccgacgtcgt gttccagtgc cgtgggcagg ccgccaccct tgggctcgcg
      481 ctgcgcctgc tacgcccgca aggcaccgtg atcgatctcg ccttccatca ggcgggcgcg
      541 gacgccttgc ggttgggcga ggagtttcac cacaacggcc tcaccctgcg cagcgcgcag
      601 atcggccggg tgccacgagg gctcgcgcac tactgggatc gggagcggct ttccaccgaa
      661 acactcgaat tgctgcgcac cgacggcccc gctatccgca agcacctggt gaccgacgtg
      721 ctatcacttg ccgaagcccc cgcgctgctc gccgacctgg acacccaccg caagcacgtc
      781 ctgcaagccg tattcacctt cgagcagtgc tgacatcccg cataatgcat ttctcgcttt
      841 agcccacacc gaactccgcg cgcagcgccg gtaatagctc atctgcggcg tagtcgagga
      901 acggctcctg gtgttgtggc gccccaccga tctgtaccaa agcaagatcc gtgaacccgg
      961 cctcggcgaa cggacgcgcc gccgccacgt gggcggcgac gtcaggcccg caggaaatgc
     1021 tctcggcgat gtcctgaggc cgcaggaatt gactggcgtc tgcgaatcct gcggtggatg
     1081 gcagctcggc gttaaccttc catccgccgg cgaaccagcg gaactgctcg tgcgccctgg
     1141 agatcgcagc atcgcggtca gcgtcgaaac agatcggcaa ttgaccgatc cgacggctgg
     1201 aacccttgtc aggccgcgcg gcgtcccaca ttctgcccag ctccggcttc ggctcgacgg
     1261 cgatcatgtg gtcggccagc ggcgcgaacg cctcgcagga ttggccgcct gacaccgcga
     1321 ccgcgatcgg cacccgctgg gtgggcaggt cccacagttt ggccgagtcg acttgataat
     1381 gctggccgcg aagggtgacg taaccgccat caaacaatgc gttgatgatg tccagtgcct
     1441 cgacaagcct ttcgtggcgc accgcgactg acggccagcc gagccccacc acatgctcgt
     1501 tgaggttctc ccccgacccc agtcccaggg t
//
LOCUS       DATADN010001120         5545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_279294, whole genome shotgun sequence.
ACCESSION   DATADN010001120 DATADN010000000
VERSION     DATADN010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279294"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VHH53_14370"
     CDS             <1..523
                     /locus_tag="VHH53_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX2291341.1"
                     /translation="LRFTFPRQRRDRRLCLADFYRPREAAMPAGEVDVLPVHLVTMGQ
                     AIADYAAELFAKDAYRDYLEVHGLGVQLTEALAEYWHRRIREELHFASGAAVAEEDPA
                     EVEEFFKLGYRGARYSLGYGACPELEDRAKIVALLQPERIGVTLSEEFQLHPEQSTDA
                     IVAHHPEAKYFNA"
     gene            584..847
                     /locus_tag="VHH53_14375"
     CDS             584..847
                     /locus_tag="VHH53_14375"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX2291342.1"
                     /translation="MKAFDELFGELTERARTRPPGSSTVAALDGGVHAQGKKVLEEAG
                     EVWLAAEHESDERLAEEISQLLYRVQVLMLGRGIDTSDVYRYL"
     gene            888..1733
                     /gene="hisG"
                     /locus_tag="VHH53_14380"
     CDS             888..1733
                     /gene="hisG"
                     /locus_tag="VHH53_14380"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX2291343.1"
                     /translation="MLRVAVPNKGTLAEPASEMLAEAGYRQRHDAKDLTVIDAANEVE
                     FFFLRPRDISVYVGSGELDLGITGRDLALDSAAPVCELLALGFGGSTFRYAAPLGRDW
                     KVDDLDGLRVATAYPRLVKADLARHGVHVDVIRLDGAVEISVQLGVADAIADVVGSGR
                     TLRQHGLGAFGDAICSSEAVLVERANAAPSPGKSQFAARLQGVVFAQQYVILDYDCPR
                     TLLDEAVVITPGLESPTLAPLADPDWVAIRAMVPRKDANRVMDELSARGAKAILASDI
                     RSCRF"
     gene            complement(1741..2640)
                     /locus_tag="VHH53_14385"
     CDS             complement(1741..2640)
                     /locus_tag="VHH53_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2291344.1"
                     /translation="MTEILASSAPASTPARRRPALSPARAADFKQCPLLYRFRAVDRL
                     PETPSRVQVRGTVVHAVLEQLFALPADQRAPEVGRGLIDPVWRDVLAGSPKLAELFAG
                     PDDPELAEWLDSARALVEAYFALEDPRLIAPEACELLVEAELPTAAGEALLLRGYIDR
                     LDVAPTGEIRVVDYKSGAAPREVFEAKALFQMKFYALVLLRIRGVVPRQLRLMYLADR
                     QALSYHPDEEELVRFERTLGAIWSAILRAAQDGDFRPNPSRMCDWCDHKPRCPAFGGT
                     PPPYPGWPDPAGAGTETARDRAE"
     gene            2721..3563
                     /locus_tag="VHH53_14390"
     CDS             2721..3563
                     /locus_tag="VHH53_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX2291345.1"
                     /translation="MTSSGPFQPGDRVQLTDPKGRRHTVLLEPGAQFHTHRGALAHDD
                     LIGLPEGSVVASAAGTQYLALRPLLADYVMSMPRGAQVVYPKDAAQILMWGDIFPGAQ
                     VLEAGAGSGALTCSLLRAVGERGSVTSYEARTDHAEHAARNVERFFGAAPPNWQLTVA
                     DLITHPADQPMDRAVLDLARPWELLPTVSAALVPGGVLTVYVAATTQLSRTTEALREA
                     GCYTEPQAWETLLRPWHVVGLAVRPEHRMVGHTAFLLTARRLAEGVVPPTPQRRPSRS
                     NRRL"
     gene            3735..5459
                     /gene="arc"
                     /locus_tag="VHH53_14395"
                     /pseudo
     CDS             3735..5459
                     /gene="arc"
                     /locus_tag="VHH53_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
BASE COUNT          914 a         1867 c         1903 g          861 t
ORIGIN      
        1 gttgcgcttc accttcccgc gccagcgccg cgaccggagg ctgtgcctgg cggatttcta
       61 ccggccccgg gaagcggcga tgcccgcagg tgaggtcgat gtgctgccgg tgcacctggt
      121 gacgatgggc caagcgatcg ccgactacgc cgccgagctg ttcgccaagg atgcctaccg
      181 cgactacctg gaggtgcacg gcctcggggt acagctgacc gaggcgctgg ccgagtactg
      241 gcaccggcgg atccgggagg aactgcactt cgcctccggg gcggcggtcg ctgaggagga
      301 cccggccgag gtggaggagt tcttcaagct cgggtaccgg ggtgcgcggt actcgctggg
      361 ctacggggcc tgcccggagc tagaggaccg cgcgaagatc gtcgcgctgc tgcagccgga
      421 acggatcggg gtcacgctgt ccgaggagtt ccagctgcat ccggagcagt ccaccgacgc
      481 catcgtcgcc caccaccccg aagccaagta cttcaatgcc taagggggtc gtgagtgcgt
      541 aacagtgtta tgcgctcaat gccactcagg aggatggtgg ctggtgaagg cgtttgatga
      601 gctgttcggg gaattgactg agcgggcccg gacccgaccg ccgggctcca gtacggtggc
      661 ggcgttggac ggcggggtac acgcccaggg caagaaggtg ctcgaggaag ccggcgaggt
      721 gtggctcgcc gccgaacacg aatccgatga gcggttggcg gaggagatct cccagctgct
      781 ctaccgcgtt caggtgctca tgctcggccg cgggatcgat accagcgacg tctaccgcta
      841 cctgtgaggg gccgatgaac ggggacatca cgaacggcga tggccagatg ctgcgggtcg
      901 cggtgcccaa caagggcacg ctggccgagc cggcctcgga gatgctggcc gaggcgggat
      961 accggcaacg ccacgacgcc aaggacctga ccgtcatcga cgcggccaac gaggtggagt
     1021 tcttcttcct gcgtccccgg gacatctccg tctacgtcgg ctccggtgag ctggatctcg
     1081 ggatcaccgg ccgggacctg gcgttggact ccgcggcgcc cgtctgtgag ctgctcgcgc
     1141 tcgggttcgg cgggtccacc ttccgctacg ccgccccgct gggccgggac tggaaggtcg
     1201 acgacctcga cggcctgcgg gtggccactg cctacccccg gctggtcaaa gcggacctgg
     1261 cccgccacgg tgtgcacgtc gacgtgatcc ggctcgacgg cgcggtggag atttcggtgc
     1321 agctcggggt ggccgacgcg atcgccgacg tggtcggctc cgggcgcacc ctgcgccagc
     1381 acggcctggg tgccttcggt gatgcgatct gctcgtcgga ggcggtgctc gtggagcggg
     1441 ccaacgccgc gccatcgccg gggaagtcgc agttcgcggc gcgcctgcag ggcgtggtgt
     1501 tcgcccagca atacgtgatt ctcgactacg actgcccgcg caccctgctg gacgaggcgg
     1561 tcgtcatcac gccagggttg gagtcaccga cgctggcgcc gctggccgat cccgactggg
     1621 tcgcgatccg cgcgatggtg ccgcgcaagg acgccaaccg ggtcatggac gagctctccg
     1681 cccgcggcgc caaggccatc ctggccagcg acatccgctc ctgccggttc tgacaacgcc
     1741 ctactccgcc cggtcgcgcg cggtctcggt gcccgccccg gccggatccg gccagcccgg
     1801 gtaaggcggt ggggtaccgc cgaacgctgg gcagcgcggc ttgtgatcgc accagtcaca
     1861 catccggctg gggttgggcc ggaagtcacc atcttgggcg gcccgcagga tcgctgacca
     1921 gatcgcgccc agtgtgcgct cgaaccggac cagctcctcc tcatcagggt ggtagctcaa
     1981 cgcctgccgg tcagcgaggt acatcagcct cagctggcgc ggcaccacac cgcggatacg
     2041 cagcaagacc agggcgtaga acttcatctg aaacagcgcc ttggcctcga agacctcccg
     2101 tggggcggcg ccggacttgt agtccaccac ccgaatctcg ccggtgggag caacgtcgag
     2161 ccggtcgatg tatccccgca gcaacagcgc ctcgccggcg gccgtcggca gctccgcctc
     2221 caccagcagc tcgcaggcct cgggtgcgat cagccggggg tcctccagcg cgaagtaggc
     2281 ctcgaccagc gcacgcgccg agtccagcca ctcggcgagc tccggatcgt cgggtccggc
     2341 gaacagctcg gccagcttgg gactgccggc cagcacgtcg cgccacaccg gatcgatcag
     2401 gccgcgcccg acttcggggg cgcgctggtc ggcaggcagc gcgaacagct gctccagcac
     2461 ggcgtgcacc acggtgccgc ggacctgcac ccggctgggc gtctcgggca accggtcgac
     2521 agctcggaac cggtacagca gcgggcactg tttgaaatca gctgcccgcg ccggggacag
     2581 tgccgggcgc cggcgcgccg gggtactggc tggggcgctg ctcgccagaa tctccgtcat
     2641 ggcttggcag ggtaggccag caccccgaca gcccagcatc ccgacagctt ccggtcggtg
     2701 atctgccacc atgagcccct gtgacctcca gcggaccgtt ccaacccggc gatcgggtgc
     2761 agctcaccga tcccaaggga cgccggcaca cggtgctgct ggagcccggc gcgcagttcc
     2821 acacccatcg cggtgcactg gcccacgacg atctgatcgg gctgcccgag ggcagcgtcg
     2881 tcgcgtccgc cgcggggacg cagtacctcg cgctgcgccc gctgctggcc gactacgtga
     2941 tgtccatgcc gcgtggagcg caagtggtct atcccaaaga cgccgcgcag atcttgatgt
     3001 ggggcgacat ctttccgggt gcgcaggtgc tcgaggccgg agctggctcc ggggcgttga
     3061 cctgctcgct gctgcgggcg gtgggggagc gtggcagcgt gacgtcgtac gaggcacgca
     3121 ccgaccatgc cgagcatgcc gcgcgcaacg tcgaacggtt cttcggggcc gcgccgccga
     3181 actggcagct gacagtggcg gacctgatca cccatcccgc cgatcagccg atggaccggg
     3241 ccgtgctgga cctggcccgc ccgtgggagc tgctgcccac ggtctcggcc gcgctggttc
     3301 ccggtggagt gctcaccgtc tatgtggccg ccaccacgca gctgtcccga accaccgaag
     3361 cgctgcgcga ggcgggctgt tacaccgaac cgcaggcgtg ggagaccctg ctgcggccct
     3421 ggcacgtggt cggcctggcg gtgcgacctg aacaccggat ggtgggccac accgcgttcc
     3481 tgctcaccgc ccgccggctg gcggagggcg tcgtgccgcc caccccgcag cgcaggccca
     3541 gccggtccaa ccggcgcctg taaatttgtt aacttcgtga tccgcgcaga atcatcgcag
     3601 catcgttcct cacagttctg gcgcgcagtc ccgtaccgtt aacggtctga gaaccttgcg
     3661 ggaggaggtg cctcgtggcg cacgatcatc ccagcgaccg gaccgccggg ggtcgggacc
     3721 gtatcgaccc cactctggcc gctcagatca gagtccttga ggacgaagtg acgctgctgc
     3781 gtcgccgcct taccgagtcc ccccggcacg tccggctgct cgaggagcgt ctcgcggagg
     3841 cgtcggcttc ggtggcacag ctcaccgagc ggaacaccaa gctggtggac acgctgcgag
     3901 atgctcgtgg tcagctcctg gccctgcgcg aggaggtcga ccggttggcc cagccgccca
     3961 gcggctacgg cgtgttcctc ggcgacttcg acgacgacac cgtggacgtg ttcacttctg
     4021 gtcgtcggat gcgggtggcg gtatcgcccg cggtcgaaat cccggcgctg cgccgcgggc
     4081 agagcgtgcg gctcaatgag gcgctgacgg tcgtggaggc cggcacgttc gagttgaccg
     4141 gcgaggtctg cgcactgcgc gaggtgctcg acggcgacca gcgggcactg gtcgtcgggc
     4201 acgccgacga agagcgggtg gtgtggctgg ccgagccgtt gatccccggg ccggccaacc
     4261 ccgacgcggt accgctcaag ccgggtgact ccctgctggt ggacaccaaa gccgcctacg
     4321 cctacgaacg ggttcccaag gccgaagtcg aggacctcgt gctggaggag gttcccgacg
     4381 tcgggtatga ggacatcggc ggcctgtcca accagatcga gcagatccgc gacgctgtcg
     4441 agctgccgtt cctgcatgcc gacctgttcc gcgaatacga gctgcgccca cccaagggcg
     4501 tgctgctcta cggtcctcct ggttgtggca agaccctgat cgccaaggca gtagccaatt
     4561 ctctggccaa gaaggtggcc caggtccgcg gagacaacga ggccaagagt tacttcctca
     4621 acatcaaggg ccctgaactg ctcaacaagt tcgtcgggga gaccgagcgg cacatccggt
     4681 tgatcttcca gcgggcccgg gagaaggctt ccgagggcac acccgtgatc gtgttcttcg
     4741 acgagatgga ctcgatcttc cgcacccgcg gatcgggggt ttcctccgac gtcgagacga
     4801 cgatcgtgcc gcagctgctc agcgagatcg atggcgtgga ggggttggag aacgtcatcg
     4861 tcatcggcgc ctccaaccgc gaggacatga tcgatccggc gatccttcgt cccggacgcc
     4921 tcgacgtgaa aatcaagatc gagcgtccgg acgccgaggc agccaaggac gtcttctcca
     4981 agtacctcaa gacgacgcta ccgctgcacg ccgacgacct cgcggaattc ggccacgatt
     5041 cccaggcctg catccaggcg atgatccagg gcaccgtcga acggatgtac gccgagagcg
     5101 aggacaaccg cttccttgag gtcacctacg caaacggcga caaggaggtc ctctacttcc
     5161 gggacttcaa ttcgggcgcg atgatccaga acatcgtgga tcgggctaag aagaaggcga
     5221 tcaagtcggt gctggagacc ggtcagccgg ggttgcggat gacccacctg ctcgattcga
     5281 tcgtcgacga gttcgccgag aacgaggacc tgcccaatac caccaacccc gacgactggg
     5341 cgcggatctc gggcaagaag ggagagcgga tcgtctacat ccgtaccctg gtcaccggca
     5401 agaacgccga ctccggtcga gcgatcgaca ccgccaccaa caccggacag tacctgtaaa
     5461 cggtcggccc acgcgcgaac gtcttgatcg cggcttgtgt gccgaaacga cacgggatgt
     5521 cgtctcggcg cacaagcgac gatct
//
LOCUS       DATADN010001121         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1050823, whole genome shotgun sequence.
ACCESSION   DATADN010001121 DATADN010000000
VERSION     DATADN010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050823"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..1483
                     /locus_tag="VHH53_14400"
     CDS             416..1483
                     /locus_tag="VHH53_14400"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2291346.1"
                     /translation="MLPEVVDGIIGADTHRDTHQLEIAFPSGAMIATRSFSNDSAGHA
                     EALTWILEHAPGPRLVLSIEGTRSYGAGLARAATAAGISVIEAEQPTRKTRRGKGKSD
                     PMDAHLAVLYAVQLDAEKLPTPRADGDREALRILLCARQELTTTSTAQTNRLKALLRD
                     GDDTDRRLACGELTLTTLAELVKRRLPRNASRQQAIRHAEIHRLTVAVRDARQILKAN
                     GAELAAIVNDLVPGLTQRPGIGPVSAAQAIVSFSHPGRCRNDAAFAKLAGTSPLEVSS
                     GHTQRHRLNRGGDRALNKALHTIARTRMRSDPATSAYVARRRAEGKSKREIRRCLKRY
                     IARQLYRTLTAAMTPDIAATV"
     gene            1607..1907
                     /locus_tag="VHH53_14405"
                     /pseudo
     CDS             1607..1907
                     /locus_tag="VHH53_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007053875.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          367 a          649 c          594 g          297 t
ORIGIN      
        1 agccgaccat gatcgacttc gacagggtta ttaaccggta tgaaagatcc gcgtcgccgg
       61 tctcgggggc tgtctgaata agggtgtaac tggcccgaca cgccgagctg tgcttggcgg
      121 gaaggatcag tcgtgaccga gacgatctgt gagtgtcgtt gcggtgctcc tgggtggagg
      181 accaccaggc tcggcgccgg tgacccaacg tgactccgag acgatctggc cgctttggcg
      241 tgccctgggc tcgacttgtc cgtggggttg ccggcaccgg cttggcccac cagttggcct
      301 gattagaagc ctgtccgcgc gagcgtgacc cgtcacagat ctgccgcttt ggtgaccgca
      361 cccaggccag tgccgacgtt gaccgtgccc acggaaggag atccggccag tggccatgct
      421 gccagaagtc gttgatggga tcatcggggc agacacgcac cgtgacaccc atcagctcga
      481 gatcgcgttt ccgagcggcg cgatgatcgc cacccgctca ttcagcaatg acagtgccgg
      541 ccacgctgag gctctgacct ggatcttgga gcatgccccg ggtccgaggc tggtgctgtc
      601 gatcgagggc acccgcagtt atggcgccgg gctggcccgc gcggccaccg ccgcggggat
      661 ctcggtgatc gaggccgagc aaccgacccg caagacccga cgcggtaagg gcaagtccga
      721 tccgatggat gcccacctgg ctgtgctgta cgccgtgcaa ctcgacgccg aaaaactgcc
      781 cactcctcgt gctgatggcg accgcgaggc cttgcggatt ctgttgtgcg cccgccagga
      841 gttgaccacc accagcaccg cccaaaccaa ccgcctcaaa gccctgctac gggatgggga
      901 tgacaccgac cgccgcctcg cctgcggcga actcaccctc accaccttgg ctgaactggt
      961 caaacgacgt ctgccccgca acgccagccg acagcaagct atccgccatg ccgagatcca
     1021 tcgcctgacg gtggcagtac gcgacgcccg tcaaatcctc aaggccaatg gcgccgagct
     1081 cgccgcgatc gtcaacgacc tcgtgcccgg gctgactcag cgacccggca tcggcccggt
     1141 cagcgcggcc caagcaatcg tgtcgttttc ccaccccggg cgctgccgca acgatgccgc
     1201 atttgccaaa ctcgccggaa ccagccccct ggaggtctcc agcggccaca cccagcggca
     1261 tcggctcaac cgcggcggag accgggcgct gaacaaagcc ctccacacca tcgcgcgcac
     1321 cagaatgcgc agcgaccccg ccaccagcgc gtacgtggct cgtcgtcgtg ccgagggcaa
     1381 gtccaaacgt gagatccgcc gctgccttaa gcgatacatc gcccgccagc tctaccgcac
     1441 cctcaccgcg gcgatgaccc ccgacatcgc ggccaccgta tgaactagcc gcgccggctc
     1501 acacgacagg aggaagcact ccaagcgatc ttgacacaaa tagaagcgtc gagcccatga
     1561 cagctaagat gatcaacagc gactcgctca ggaactcgtg gagaaggccc gtgctgaggg
     1621 tgtggagatg gttggccagg gtagcttgct caccgggctg accaagtcgg tgttcgagac
     1681 cgccctggag cggaactgac cgagcacctc ggctatgaca agcacgaccc gatgggccgc
     1741 aacggcgcta gctcccgcaa cggcacccgc actaagacgg tggtcaccga gatcggcccg
     1801 gtcgagatcg aggtgccccg ggatcgggag ggcagttttg acccggtgat cgtgcgcaag
     1861 cgtcagcggc ggctagagag catcgatgcg atcgtgttgt cgctgac
//
LOCUS       DATADN010001122         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_122731, whole genome shotgun sequence.
ACCESSION   DATADN010001122 DATADN010000000
VERSION     DATADN010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122731"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1021
                     /gene="panB"
                     /locus_tag="VHH53_14410"
     CDS             158..1021
                     /gene="panB"
                     /locus_tag="VHH53_14410"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2291347.1"
                     /translation="MTAAAVEPNLYGGPIDPDRKAKRIRVQDLLAAKRRGEKWAMLTA
                     YDYSTAALLDAAGIPVLLIGDSAANVVYGYETTVPVTVDELIPLVRGVARGARRALVI
                     ADLPFGSYQSSPAQALHTAARFLKESGAHAVKLEGGRRVIPQIEALAEAGVPVMAHLG
                     LTPQSVHALGGYSVQGRGEQGERLVEDAKALEAAGVFAIVLEVVPAELAARISAATVV
                     PTIGIGAGPDCDAQVLVWQDMAAFRVPGGRTPKFVKTYAELGDALREAACHFAEDVRS
                     GAYPGPEHAYG"
     gene            complement(1092..1490)
                     /locus_tag="VHH53_14415"
     CDS             complement(1092..1490)
                     /locus_tag="VHH53_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="HEX2291348.1"
                     /translation="MVSELPADAQRWTAAERELARQVLLSRTVVVNVRRGGPHRHLVP
                     WLRDRGMITYVGHAGSRHSWPRSDFANPFVKQASTDREAMVRRYSAWLDDQPELLARI
                     SAGELTGRALGCWCAPQPCHADVLAERTMS"
     gene            complement(1633..1950)
                     /locus_tag="VHH53_14420"
     CDS             complement(1633..1950)
                     /locus_tag="VHH53_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291349.1"
                     /translation="MPEGSAAQQAQALLPECRVTAAFHHVSAVLLEDPEVPSLDIDVL
                     VLGDDCEATDLVCALANAIPGGRGIYGGRLRNAGQVEALTANLVAINKRYHTHAGIRI
                     TDL"
BASE COUNT          335 a          696 c          727 g          322 t
ORIGIN      
        1 gatcaccagc cgctggcgcg aacgatcccc cgagctgtga gcctcgtcgc acgctgatgc
       61 cgtgccccga ccctggtgcc aggcttgggg tatcagccat ccggcacaac ccggggaccg
      121 gcgacgaact gccggcctcg agggaggacg gtcaacgatg accgcagctg ctgtcgagcc
      181 caacctgtac ggcggaccga tcgatccgga ccggaaagcc aagcggatcc gggtccagga
      241 ccttctcgcg gccaaacggc gcggagagaa gtgggcgatg ctcaccgcct acgactactc
      301 caccgcggct ctactggatg ccgcgggcat accggtgttg ctgatcggtg actccgcggc
      361 caacgtcgtg tacggctatg agacgacggt gccggtgacc gtcgacgaac tgatacccct
      421 ggtgcgtggt gtggcccgcg gggcacgccg ggcgctcgtc atcgctgacc tgcccttcgg
      481 ctcctaccag tcctcaccgg cgcaggctct gcacactgcg gcacgtttcc tcaaagagtc
      541 cggcgcccat gcggtgaagc tggagggtgg tcgccgggtg atacctcaga tcgaggcgct
      601 ggctgaggcc ggcgtgccgg tgatggccca cctcgggctc accccgcagt cggtgcacgc
      661 cttgggcggt tactcggtgc agggacgtgg cgagcagggc gagcgactgg tggaggacgc
      721 gaaggctctg gaagcggcgg gcgtgttcgc catcgtcctg gaggtggtgc ccgccgaact
      781 cgccgcccgg atcagcgcag cgaccgtggt acccaccatc ggcatcggcg cggggcctga
      841 ctgcgacgcc caggtcttgg tgtggcaaga catggcggcc ttccgcgttc ccggcggccg
      901 tacgccgaag ttcgtcaaga cttacgcgga actcggtgac gcgctgcggg aggcagcttg
      961 tcacttcgcc gaagatgtcc gctcaggcgc gtacccgggc ccagaacacg cctacggctg
     1021 agcatgcccc ctcggcgtgt tcggctccta tgcccggtgt gtcgtgggct tgtgcacagg
     1081 gttggatgct gtcacgacat tgttcgctcg gcgaggacgt cggcatgaca gggttgcggc
     1141 gcgcaccaac agcccagcgc tcgccccgtc agctctccgg cgctgatccg agcgagcaac
     1201 tccggttgat cgtcgagcca cgcactgtag cggcgcacca tggcctcccg gtcggtgctg
     1261 gcctgcttca cgaacgggtt ggcgaagtcc gaccgtggcc aggaatggcg tgaccccgcg
     1321 tggccgacat aggtgatcat gccgcggtcg cgcagccacg gcacgaggtg gcgatgtggg
     1381 ccgccgcgac gcacgttcac caccacggtc cggctgagga gcacctgccg ggccagctcc
     1441 cgttcggcgg cggtccaccg ctgggcatcg gcgggcagtt cggagaccac gagcgaacac
     1501 gttagaagac ctagcctgtc gggccgatcc ggtgtgctcc agatgcggac acaccgtacc
     1561 tgcgagccgg acacaccggc acgagagaca gacacgccgg gcaggagagc cggacacgcc
     1621 ggggggtaga ggttagaggt cggtgatccg gatcccggcg tgggtgtgat accgcttgtt
     1681 gatcgcgaca aggttggcgg tcagcgcctc cacctgaccg gcattgcgca gccgcccacc
     1741 gtagatgccg cgcccaccgg gtatcgcgtt ggcgagcgcg cacaccagat cggtggcctc
     1801 gcagtcgtcg cccagtacca gcacgtcgat gtcgaggcta ggcacctcgg ggtcctcgag
     1861 cagcaccgca gagacgtggt gaaacgccgc agtcacgcgg cactcgggca acagggcctg
     1921 ggcttgctgg gcggcgctgc cctcgggcac ggcatgcacg atcgtgattg actgcgtgaa
     1981 cccgctcgga ttcgacaaac gaggcccgtt cgtgcacgcc gtgcccgagg gcagcgccgc
     2041 ccagcaagcc caggccgtgt tgcccgagtc ccgcgtgact
//
LOCUS       DATADN010001123         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1332990, whole genome shotgun sequence.
ACCESSION   DATADN010001123 DATADN010000000
VERSION     DATADN010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332990"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VHH53_14425"
     CDS             <1..819
                     /locus_tag="VHH53_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2291350.1"
                     /translation="GAVLLPFFAFTSFLIAIPTGIKFFNWIGTMWRGQLTFETPMLFS
                     IGFLITFLLGGLTGVLLAAPAIDFHVTDTYFVVAHFHYVLYGTIVFATFAGIYFWFPK
                     MTGRMLNEPLGKFHFWATFIGFHLTFLVQHWLGNEGMPRRYADYLPSDEFTTLNMVSS
                     IGAFVLGASTLPLLWNAFRSYRYGEVVTVDDPWGFGNSLEWATSCPPPRHNFTELPRI
                     RSERPAFELHYPHMAVRMRAEAHIGGTGHPTAPDEIAAQAVQRGDTGSTNPRDK"
     gene            1226..>1735
                     /locus_tag="VHH53_14430"
     CDS             1226..>1735
                     /locus_tag="VHH53_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291351.1"
                     /translation="MATQNNHICGTVHEGEKRSFDTEADRTDVLLNAVMNRGLRLTTD
                     LPRLALRPRPGWAVHLNPAGTLTLTWPHVRSLLLDAPLNLPTGWREAAAEHGLVLLFV
                     GDRLGLGDHTEGHQTNPGKRALQAAERGELAAGAIAYPRPQLEPRPVRQADPQPRSRP
                     HRWARTHRWH"
BASE COUNT          314 a          613 c          512 g          296 t
ORIGIN      
        1 ggtgcggtgc tgctgccctt tttcgcgttc accagtttcc tcatcgcgat cccgaccggg
       61 atcaagttct tcaactggat cggcaccatg tggcggggac aactcacgtt tgagacaccg
      121 atgctgttct cgatcggctt cctgatcacc ttcttgctcg gtggactaac cggagtgctg
      181 ctggccgccc cggcgatcga cttccacgtc accgacacct acttcgtggt ggcgcacttc
      241 cactacgtgc tttatggcac gatcgtgttc gccaccttcg ccggcatcta cttctggttc
      301 ccgaagatga ccgggcggat gcttaacgag ccgctaggca agtttcactt ctgggccacg
      361 ttcatcgggt ttcacttgac gtttctggtg cagcactggc tgggtaacga gggcatgccc
      421 cgccgctacg ccgactacct gcccagtgat gaattcacca cgctgaacat ggtgtcctcg
      481 atcggtgcct tcgtcctggg cgcctccaca ctgccacttc tgtggaacgc attccgtagc
      541 taccgctatg gcgaggtcgt caccgtggac gacccgtggg gattcggtaa ctcgctggag
      601 tgggcgacct cgtgtccacc gccgcggcac aatttcactg aactgccccg gatccgttcc
      661 gagcgacccg ccttcgagct gcactacccg catatggcgg tccggatgcg agccgaggcg
      721 cacatcggtg gcaccggaca tccgaccgcg ccggacgaaa tcgcagcgca ggccgtgcaa
      781 cgaggcgaca ccggcagtac caacccgcgc gacaagtgac cggccacccc atgcacgacc
      841 ctgctggcca cggtgaccag cgggaccaac ccgacggagc acgagggaac cgagtgttgg
      901 tgggtcgcct gtggccgcgc aggatgagca aatccaaagc cgacatcggc gagtgatgca
      961 agcaggcgcg ctgtcgacgg tgttacgcaa ttggtaagca tgacccgcag gtccgccgag
     1021 ttccgtcgcc gctaccggac cgacctcgcc tcaggggagc aggcgacggc cctggcgcac
     1081 ttgcatcttt gcgccggtgg cgtccccgcg actgccactg cggtgactgt ggctcggtcg
     1141 tcgctccgct tccagctccc aaccccacca tcggcggaga ggacgaggcg atgcacgcga
     1201 tgacgctgca cgcgcaagcc ggtctgtggc aacacagaac aaccacattt gcggcactgt
     1261 tcatgagggc gaaaagcggt cattcgacac cgaggccgat cgcaccgacg tgctgctcaa
     1321 cgcggtcatg aaccgcggac tgcgcttaac caccgacctg ccccggctag cgctgcgccc
     1381 gcgtcccggc tgggcagtac acctcaaccc agccggcaca ctgactctca cctggcccca
     1441 cgtccgatcc ctacttctcg acgcgccgct gaacctgccc accggctggc gcgaggcagc
     1501 agcagaacac ggcttggtgt tgctcttcgt cggtgacagg ctcggcctgg gcgaccacac
     1561 cgaaggccac cagaccaatc ccggcaaacg cgcgctacaa gccgccgagc gaggggaact
     1621 agccgccgga gcgatcgcct acccgcgccc gcagctagaa ccccgcccag tgcgccaggc
     1681 cgacccccag cccagaagcc gacctcaccg atgggcccgc acacatcggt ggcac
//
LOCUS       DATADN010001124         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1051378, whole genome shotgun sequence.
ACCESSION   DATADN010001124 DATADN010000000
VERSION     DATADN010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051378"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VHH53_14435"
     CDS             complement(<1..945)
                     /locus_tag="VHH53_14435"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX2291352.1"
                     /translation="MRVLVVNVGSSSLKLSVLSPDGTVQAATTVQRWAGRTHLEPIHQ
                     FLEGTDRINAVGHRVVHGGPRYRAPAWIDDELVDYLDSIRDLAPLHNPRALAGIRVVT
                     DLLPQTPAVACFDTAFHATLPAAAHTYALPREWNQRWGLRRYGFHGLSHAHAARRGAE
                     LANATMPGSRMVSCHLGAGASLAAVRGGICVDTTMGFTPLAGLVMVTRSGDVDPGLLL
                     WLLQHGGVSVGELNQALENRSGLKGLSGTSGDLRDVLAGRATGDPDCTLAFDVFIHRL
                     CREIAAMTAAVGGLDVLVMTGGIGEHAPLVRTQVATRLT"
     gene            complement(948..>2455)
                     /locus_tag="VHH53_14440"
     CDS             complement(948..>2455)
                     /locus_tag="VHH53_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX2291353.1"
                     /translation="SWRSHQVPFSNARGDDSHRAVLEQWMRGYRPQELFDEAGAPVPG
                     IRDLHPGGRRRMSANPHSNGGAVMRDLRMQDFRDYAVAVEAPGTGAVESTRVLGSFLR
                     EVMRDNMTSFRVFAPDENNSNRLSAVLEVTGRTWNARMQPDDEDLAVDGRVMEMLSEH
                     TCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQHAKWLKTTNGIAWRRPIASLNYLL
                     TSHVWRQDHNGFSHQDPGFIDHVVNKKSDVIRVYLPPDANTLLSVVDHCLGSRQYVNV
                     IVAGKQPQLQYLTMQDAIVHCTKGIGIWEWASTDAGTEPEVVLGCAGDVPTMETLAAA
                     ELLRGWFPDLRVRVVNVVDLMRLQDDRDHPHGLSDREFDALFTTDKPNIFAYHGYPWL
                     IHRLTYRRTNHDQLHVRGYVEEGTTTTPFDMCVLNRIDRFNLAIDVLDRVPRLQAISA
                     HHREHLRNKLIEHRQHIRTYGQDMPEIRDWQWSAGPGPTTQVNNPPLTEPV"
BASE COUNT          402 a          824 c          833 g          396 t
ORIGIN      
        1 cgttaggcgg gtcgcgacct gggtccggac gagcggggcg tgctcgccga tgccgccggt
       61 catcaccagc acgtccaacc cgcccaccgc ggcggtcatc gccgcgatct cccggcacaa
      121 ccggtggatg aacacgtcga atgccagtgt gcagtcgggg tcgccggtgg cccggccggc
      181 gaggacgtca cgcaggtcac cggaggtacc cgacagtccc ttcaggcccg accggttctc
      241 cagcgcctgg ttgagctcac cgacggacac accgccatgc tgcagcagcc acagcagcag
      301 accggggtcc acgtccccgg agcgggtcac catcaccagc ccggccagtg gggtgaaccc
      361 catcgtcgtg tcgacgcaga tgccgccgcg gaccgcggcc agcgatgcac cagcaccgag
      421 atggcaggac accatccgcg aacccggcat cgtggcgttg gcgagttccg cgccccggcg
      481 ggccgcgtgg gcatgggaga gcccgtgaaa accgtagcgg cgcagccccc agcgctggtt
      541 ccactcccgc ggcagcgcat aggtatgcgc cgcggcgggc aacgtggcgt gaaaagcagt
      601 gtcgaagcag gcgaccgccg gggtctgtgg cagcaggtcg gtcacgaccc ggatcccggc
      661 gagagcccgg gggttgtgca gcggcgcgag atcgcgaatc gagtccaggt agtcgaccag
      721 ctcgtcgtcg atccaggcgg gtgcccggta ccggggccca ccgtgcacga cgcggtggcc
      781 gacggcgtta atccggtcgg tgccctcgag gaactgatgt atcggctcga ggtgagtgcg
      841 gcccgcccag cgttgcaccg tggtggcggc ctggacggtc ccgtccgggg acaggaccga
      901 aagcttcagg ctgctggaac cgacgttcac caccaggacg cgcatcgcta caccggctcg
      961 gtcaacggcg ggttgttgac ctgcgtggtc ggacccgggc ccgccgacca ctgccagtcg
     1021 cggatctcgg gcatgtcctg tccataggtg cggatgtgct ggcggtgctc gatcaacttg
     1081 ttccgcagat gctcgcgatg gtgggcagag atcgcctgca ggcgtggcac ccggtccagg
     1141 acgtcgatgg ccaggttgaa ccggtcaatc cggttcaaca cgcacatgtc gaacggtgtg
     1201 gtggtcgtgc cctcctccac gtagccgcga acgtgcaact ggtcgtggtt ggtgcgccgg
     1261 taggtcaggc ggtggatcag ccacgggtac ccgtggtagg cgaagatgtt cggcttatcg
     1321 gtggtgaaca gcgcatcgaa ctcccggtcc gacaacccgt gcggatggtc ccggtcgtcc
     1381 tgcagccgca tcaggtccac cacgttcacc acccgcaccc gcaggtccgg gaaccagcca
     1441 cgcagaagct cggcggcggc gagggtctcc atcgtgggca cgtccccggc acagcccaac
     1501 accacctcgg gctcggtgcc ggcgtcggtg ctggcccact cccagatccc gatgcccttg
     1561 gtgcagtgca cgatcgcgtc ttgcatggtc agatactgca gctgcggctg cttgccggcg
     1621 acgatcacgt tgacgtactg ccggctgcca aggcagtggt cgaccaccga cagcagtgtg
     1681 ttcgcatccg gtggcaggta tacccggatc acgtcggact tcttgttcac cacgtggtcg
     1741 ataaagcccg ggtcctggtg ggaaaacccg ttgtggtctt ggcgccacac gtggctggtg
     1801 agcaggtagt tcagcgacgc gatcgggcgc cgccacgcga tgccgttggt ggtcttcagc
     1861 cacttggcat gctggttgaa catcgagtca acgatgtgga tgaacgcctc gtagcaggaa
     1921 aagaatccgt gccggccggt cagcaggtag ccctccagcc agccctggca ggtgtgctcg
     1981 gacagcatct ccatcacccg cccgtcgacg gcgagatcct cgtcgtctgg ttgcatccgg
     2041 gcgttccagg tccgtccggt gacctcaaga acggcgctga gccggttgga gttattctcg
     2101 tcaggcgcaa agacccggaa gctggtcata ttgtcgcgca tcacctcacg taggaagctg
     2161 cccagcaccc gggtcgactc caccgcaccg gtgccgggtg cctccaccgc aacggcgtag
     2221 tcgcggaaat cctgcatccg caggtcgcgc atcaccgccc cgccgttgct gtgcgggttg
     2281 gcgctcatcc gccgacgacc ccccgggtgc aggtcgcgga tcccgggcac cggagcgccg
     2341 gcctcgtcga agagctcctg cggtcggtag ccacgcatcc actgctctag caccgcccgg
     2401 tgggagtcgt cgccgcgagc gttgctgaac ggcacctgat gcgagcgcca ggagc
//
LOCUS       DATADN010001125         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_448781, whole genome shotgun sequence.
ACCESSION   DATADN010001125 DATADN010000000
VERSION     DATADN010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448781"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /gene="coaE"
                     /locus_tag="VHH53_14445"
     CDS             complement(<1..1181)
                     /gene="coaE"
                     /locus_tag="VHH53_14445"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX2291354.1"
                     /translation="MLRVGLTGGIGSGKSTVAARLAQRGAWIIDSDRIAREVVEPGTD
                     GLRAVVDEFGVEVRTEAGALDRSALGARVFGDADARSRLNAIVHPLVAARTAELLDAA
                     PPDAIVVQDIPLLVEAGLAAGFPLVVVVHAAAEVRVRRLVDQRGMPAADARARIAAQA
                     TDEQRHAAADVCLDNAGSRDETLAAVDQLWAQRLVPFEDNLRHRRRAPRPPAPILVAP
                     DDSWPWQAQRVMARVAAVAAGRAVRIDHIGSTSVPGLDAKDVLDVQVVVADLDVAKQL
                     ADDLIGAGLIRLTGHWWDNAPDGGTWGKAIAVNADPARAVNCHIRPINSPTWRQTVLL
                     RDWLRAHPSSARKYAEVKRRLAARPWDSIDAYADAKTPFVHAALDRAQQWAGRTGWLV
                     E"
     gene            complement(1204..>1993)
                     /locus_tag="VHH53_14450"
     CDS             complement(1204..>1993)
                     /locus_tag="VHH53_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX2291355.1"
                     /translation="FVDLGGVDGLVHVSELSWKHIDHPSEVVEVGQEVTVEVLDVDLD
                     RERVSLSLKATQEDPWRQFARTHQIGQIVPGKATKLVPFGAFVRVEEGIEGLVHISEL
                     AERHVEIPEQVVQVGDDVMVKVIDIDLDRRRISLSLKQANEGLSPEMEFDPTQYGMTA
                     EYDTEGNYIYPEGFDPETNDWLDGYEKQRQEWERSYAEAQVRFEQHRKQVVKAAEAQS
                     DAAEDQNYSSATAEAPTPSGGPGAGSLASDEQLAALREKLSGGV"
BASE COUNT          306 a          716 c          644 g          327 t
ORIGIN      
        1 cactccacca gccagccggt tcgccctgcc cactgctgcg ctcgatccag agctgcgtgc
       61 acgaagggcg ttttcgcgtc ggcgtaggcg tcgatgctgt cccagggccg tgccgccaag
      121 cgacgcttga cctcggcgta cttgcgagca cttgacggat gggcccgcaa ccagtcccgc
      181 agcagcacgg tttgccgcca ggtcggggag ttgatgggtc ggatgtggca gttgacggcg
      241 cgcgccggat cggcgttgac cgctatcgcc ttgccccagg tgccgccatc cggggcgttg
      301 tcccaccagt ggccggtaag tctgatcaat cccgcgccga tgaggtcatc ggccaattgc
      361 ttggccacat cgaggtccgc gacgaccacc tgcacatcga gcacgtcctt ggcgtccaga
      421 ccaggcaccg aggtcgagcc gatgtggtcg atgcgcactg cccgcccggc ggccacagct
      481 gcgacacgtg ccatcacccg ctgcgcctgc cacggccaac tgtcgtcggg cgcaacgagg
      541 atcggggcag gaggccgcgg tgcccggcgg cgatgccgga ggttgtcctc gaagggcacc
      601 agccgctgcg cccatagctg atccactgcg gcgagggtct cgtcccggga accggcgttg
      661 tccaggcaca catccgcggc ggcatggcgc tgttcgtcgg tggcttgcgc cgcgatccgc
      721 gcgcgggcgt ccgccgcagg catcccgcgc tggtccacca gacgccgaac ccgtacctca
      781 gcggctgcgt gcactacaac gaccagtgga aaccccgctg ccaaccccgc ctcgaccagc
      841 agcgggatgt cctgcacgac gatggcatcg ggcggtgccg cgtcgagcag ctcggcggtg
      901 cgcgctgcga ccagcgggtg cacgatggcg ttgagccggc ttcgggcgtc cgcgtcaccg
      961 aacacccgcg cgcccaacgc cgaccgatcc agcgcgccgg cctctgttcg gacctccacg
     1021 ccgaactcgt ccaccacggc gcgcagtcca tcagtaccgg gctcgaccac ctcgcgggcg
     1081 atccgatctg agtcgatgat ccacgccccc cgctgcgcca gccgggccgc caccgtggac
     1141 ttaccggatc cgatcccccc agtaagtccg accctcagca ttgcgcaaca gtgagtacct
     1201 gaactacacg cctccggaga gcttctcccg cagtgccgcc aactgctcgt cgctggcgag
     1261 cgaaccggcg ccagggccgc cgctgggggt gggcgcttcg gcggtcgccg aggagtagtt
     1321 ctggtcctca gccgcgtccg actgggcctc ggctgccttg acgacctgct tgcggtgctg
     1381 ctcgaaacgg acctgagcct cggcgtagga acgctcccac tcctggcgct gcttctcgta
     1441 gccgtccagc cagtcgttgg tctccggatc gaagccctcg gggtagatgt agttgccctc
     1501 ggtgtcgtac tcggcagtca tgccgtactg ggtcgggtcg aactccattt cgggggacag
     1561 cccctcgttg gcctgcttga gggacaggga gatccgccgc cggtccaggt cgatgtcgat
     1621 gaccttgacc atcacgtcgt caccgacctg cacaacctgc tcggggatct cgacgtgccg
     1681 ctcagccagc tcggagatgt gcaccaaacc ctcgatgccc tcctcgaccc ggacgaatgc
     1741 accgaaaggc accagcttgg tggccttgcc gggcacgatc tgcccgatct ggtgagtgcg
     1801 ggcgaactgg cgccacgggt cttcctgagt cgccttgagc gacagggaca cccgctcgcg
     1861 gtccaagtcg acgtcgagca cctcgacggt gacctcctgg ccgacctcga cgacctcgct
     1921 cgggtggtcg atgtgcttcc aggacagctc ggagacgtgg accagaccgt cgactccgcc
     1981 gaggtccacg aat
//
LOCUS       DATADN010001126         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1501903, whole genome shotgun sequence.
ACCESSION   DATADN010001126 DATADN010000000
VERSION     DATADN010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501903"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VHH53_14455"
     CDS             complement(<1..758)
                     /locus_tag="VHH53_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX2291356.1"
                     /translation="MVRIAVIVMMSALFAGCGGAQQASAPARDMVRPADSLLALALAG
                     EQPRALTDPSGVARQITVAETAVRDPATSPELVAAAGQTAQVAYLALLDRPDWDAAVL
                     ADVPAPLQDPVRRNVAAGREFRAMVSRLPTTVPAWRIVEPLPVPELRAYYAQAQQRFG
                     VPWSVLAAVHLVETRMGRIVGLSSAGAQGPMQFLPATWARYGLGGDVWNTRDAIFGAA
                     NYLAANGAADGTDARLDNALYRYNNDNRYVRGVR"
     gene            complement(777..1214)
                     /locus_tag="VHH53_14460"
     CDS             complement(777..1214)
                     /locus_tag="VHH53_14460"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX2291357.1"
                     /translation="MLREAVLGALVLGVTALLVNAEPGRTATAASPGPAHQVISYDTG
                     EPGGQGWLTVDIDPAVTGPAMISVTVQDSTAGARDVAELTATLTLTSTRLGPFRVPLQ
                     RTAPGRYVATGFQIPADGTWLLAVTVRTSDIDQTTVTTPIDVG"
     gene            complement(1204..>1582)
                     /locus_tag="VHH53_14465"
     CDS             complement(1204..>1582)
                     /locus_tag="VHH53_14465"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX2291358.1"
                     /translation="HLLAMGGWLGGLVTLAVVMRPAAAASAELPAAVYRFSPIATGSV
                     LLLISTGSYQSWRQLGSWAAFGSTDYGRLLLAKLVIVALLFGAAVMSRTWVARHRSTR
                     WYRCRCGHAIVPRGTNTAAQRAA"
BASE COUNT          268 a          552 c          546 g          216 t
ORIGIN      
        1 tgccgcactc cccggacgta gcggttgtcg ttgttgtagc ggtacagggc attgtccaga
       61 cgagcatcgg tgccatcggc ggcaccgttg gcggccaggt agttggccgc accgaaaatc
      121 gcgtcgcggg tgttccatac atcgccgccc aggccgtatc gcgcccacgt ggccgggagg
      181 aactgcatcg gtccctgcgc accggcgctg gacagcccga cgatccgacc catcctggtt
      241 tcaaccagat gcaccgcggc cagcaccgac cacggcacgc cgaaacgctg ctgagcctgc
      301 gcgtagtagg cccgcagctc cggtacgggg agcggctcga cgatccgcca ggccggcacg
      361 gtcgtcggca accgactgac catcgcccgg aactcgcgtc ccgccgcgac gttgcgtcgc
      421 accgggtcct gcaatggtgc cggaacgtcg gccagcacag cggcgtccca gtctggccgg
      481 tcgagaagcg cgagataggc cacctgcgcg gtctgaccgg cagcggcgac cagctccggc
      541 gaagtcgccg ggtcccgcac ggcggtctca gcgacagtga tctgccgggc tacaccgctc
      601 ggatcggtca aggcccgcgg ctgctcaccg gccagcgcca gcgcgaggag cgaatcggcc
      661 ggacggacca tgtcgcgagc cggcgcgctc gcctgctgcg cacccccgca tccggcaaac
      721 agggccgaca tcatcacgat cacagctatc cgaaccacgg agcctcacgt taccggctag
      781 ccgacgtcga tcggtgtggt cacggtggtc tggtcgatgt cggaggttcg cacggtcact
      841 gccagcagcc acgttccgtc ggccggaatc tggaacccgg tggcgacata acgccctggt
      901 gcggtgcgct gcagggggac ccggaacggg ccgagccgcg tggaggtgag ggtcaaggtg
      961 gcggtgagct cagccacgtc gcgtgcgcct gcggtggaat cctgcacagt gacactgatc
     1021 atggcaggtc cggtgacggc aggatcgatg tcaacggtga gccagccctg gccgccaggc
     1081 tccccggtgt catagctgat gacctggtgt gccgggcccg gtgacgcagc cgtcgcggtt
     1141 cgaccgggtt cggcgttgac cagcagcgcg gtgacgccga gcacgagggc gccgagcacg
     1201 gcttcacgca gcacgctgcg ccgcagtatt ggtaccgcgg gggacgatcg cgtggccgca
     1261 ccgacaccga taccaacggg tgctccgatg ccgggcgacc cacgtgcgcg acatcaccgc
     1321 ggcgccgaac aagagcgcga cgatcacgag cttcgccagc agcaaccgac cgtagtcggt
     1381 ggatccgaac gctgcccagc taccgagctg gcgccacgat tggtagctgc cggtgctgat
     1441 gagcagcagg accgacccgg tcgcgatcgg cgagaaccgg tacaccgctg ccggtagctc
     1501 ggcactcgcg gccgccgccg gacgcatgac gacggcgagc gtgaccaggc ccccgagcca
     1561 cccgcccatc gccagcagat gc
//
LOCUS       DATADN010001127         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1051770, whole genome shotgun sequence.
ACCESSION   DATADN010001127 DATADN010000000
VERSION     DATADN010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051770"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VHH53_14470"
     CDS             <1..289
                     /locus_tag="VHH53_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2291359.1"
                     /translation="ESVDWNSRLERGDVVEVARRLKAETDGLMEVGGATLAAPLVQAG
                     LVDEFQVVVTPVAVGGGTPFLPALDRWLKLELVENRTFPSGSVLLRYVPVP"
     gene            complement(304..1437)
                     /gene="ispG"
                     /locus_tag="VHH53_14475"
                     /gene_synonym="gcpE"
     CDS             complement(304..1437)
                     /gene="ispG"
                     /locus_tag="VHH53_14475"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2291360.1"
                     /translation="MPAAPPPVLAPRRPTRQLLVGKVGVGSEYPVSVQSMTTTVTADV
                     NATLQQIAELTAAGCDIVRVACPSQDDAEALAAIAGKSQIPVIADIHFQPKYVFAASE
                     AWCAAVRVNPGNIKKFDDKVKQIAAAARAKGTPIRIGVNAGSLDPRLLAKHGKATPEA
                     LVESALWEASLFAEHDFHDIKISVKHNDPVVMVRAYELLAEQCDYPLHLGVTEAGPAF
                     QGTIKSAVAFGALLRHGIGDTIRVSLSAPPVEEVKVGTQILQSLNLRPRKLEIVSCPS
                     CGRAQVDVYKLADEVTAGLEGMEVPVRVAVMGCVVNGPGEAREADLGVASGNGKGQIF
                     VRGQVIKTVPEHKIVETLIEEAMRIAEEAGEQAETGSPTVTVS"
BASE COUNT          252 a          580 c          477 g          253 t
ORIGIN      
        1 cgagtcggtc gactggaact cgcggctgga gcggggtgac gtggtcgagg tcgccaggag
       61 gctcaaggcc gagaccgacg ggctgatgga ggtgggcggc gccacgctcg ccgcgccgct
      121 ggtccaggcc ggtctcgtcg acgagttcca ggtggtggtc acgccggtcg cggtcggcgg
      181 cggcaccccg ttcctcccgg cgctggacag gtggctgaag ctggagctgg tggagaaccg
      241 caccttcccg tcgggctcgg tcctcctccg ctacgtcccc gtgccctgaa tggggggttc
      301 agctcagctg accgtgacgg tgggtgagcc ggtctcggcc tgctcgcccg cctcctcggc
      361 gatgcgcatg gcctcctcga tgagggtctc cacgatcttg tgttcgggca cggtcttgat
      421 cacctggccg cggacgaaaa tctggccctt gccgttgccg gaggccacgc ccaggtcggc
      481 ctcgcgggcc tcccccggcc cgttcaccac gcagcccatg accgccaccc gcaccggcac
      541 ctccatgccc tccagcccgg cggtcacctc gtcggcgagc ttgtacacgt ccacctgggc
      601 gcggccgcag gacgggcagg acacgatctc cagcttgcgg ggccgcaggt tcagcgactg
      661 caggatctgc gtgccgacct tgacctcctc gaccggcggc gccgacagcg acacccggat
      721 cgtgtcaccg atgccatgcc gcagcagcgc cccgaaggcc accgccgact tgatcgtgcc
      781 ctgaaacgcc ggcccggcct cggtcacccc cagatgcagc ggatagtcac actgctcggc
      841 cagcaactca taggcgcgca ccatgaccac cggatcattg tgcttgaccg agatcttgat
      901 gtcgtgaaag tcgtgctcgg cgaacaggct cgcctcccac agcgccgact ccaccagcgc
      961 ctccggcgtc gccttaccat gcttggccag caaccgcgga tccaacgagc cggcattgac
     1021 cccgatccgg atcggcgtgc ccttcgcccg cgccgcagcg gcgatctgtt tgaccttgtc
     1081 gtcgaacttc ttgatgttgc ccgggttcac ccgcaccgcc gcacaccacg cctcgctcgc
     1141 ggcgaagacg tacttgggct ggaaatggat atcagcgatc accggaatct gcgacttgcc
     1201 cgcaatcgcc gccaacgcct cagcatcatc ctgcgacggg cacgccaccc gcacgatgtc
     1261 acacccggca gcggtcaact cagcaatctg ctgcaacgtg gcattgacat ccgccgtcac
     1321 cgtcgtcgtc atcgactgca ccgacaccgg atactcacta cccacaccga ccttccccac
     1381 cagcaactga cgggtcggtc gacgcggcgc cagcacggga ggtggcgccg ccggcatccc
     1441 cagcatgatg ctgtcggtca cgattccaca gtactggcgg ctactgcccg atcctgatcg
     1501 ggttcaccag atcggctgtc accgtgagca gaaccactgc gccgcccagg aaaaccgcga
     1561 cc
//
LOCUS       DATADN010001128         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1333650, whole genome shotgun sequence.
ACCESSION   DATADN010001128 DATADN010000000
VERSION     DATADN010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333650"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VHH53_14480"
     CDS             complement(<1..1281)
                     /locus_tag="VHH53_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291361.1"
                     /translation="MFTENDTDVDQSTPGHLKALLPPVLIAGAGTAIGLAIARGARDT
                     GVPIVGASLTPSAPTCRSKLWHSVLPVSAPTTEAWLAMLRAAHARYGPMVLFPADDIV
                     VRIVAEHAAELATQFDFVLPDLPTVDRLLDKSVFHEWALANDFPVPHTAIVTTPGQLR
                     AALREMTFPVVFKPVERTPQWQDFSRWGTAYRLHTADDFDRLPFAPFDAADRFVVQEW
                     IPGRDSDVYFCLTYRNRNGEELAAQGGRKITQWRVDTGSTALAVTHQDMELHKLTRRL
                     LDTAGHVGFGSLEIRRSTRDGRMIITEPTVGRPNLQTALAAAAGVNLVDMAYRDALRL
                     PAPPSRPRREAIWIHETTFPRSVLVAARRRRLDSRTLLAALRIRRAPTGAFYSDRDPL
                     PLLFETAKVANKTLQGALTIARKSRNRRTLPQCNP"
BASE COUNT          265 a          520 c          595 g          299 t
ORIGIN      
        1 cggattgcac tgcggcagcg ttctccgatt ccgggatttc cgagcgatgg tcagtgcccc
       61 ctggagggtc ttgttcgcga ccttggcggt ctcgaagagg agtgggagcg gatcgcggtc
      121 ggagtagaac gcgccggtgg gtgcccgcct aatgcgcaac gcggcgagga gcgtgcggct
      181 gtcgaggcgg cggcggcgtg ccgcgacgag caccgaccgc ggaaaggtgg tctcgtgaat
      241 ccagatcgcc tcccggcggg gtcgactcgg tggcgcgggc agcctcagtg cgtcgcgata
      301 cgccatatcc accaggttca ccccggcggc agcggccagt gcggtctgca gattgggtcg
      361 gcccacggtc ggctcggtga tgatcatccg gccatctcgg gtgctgcgcc gaatctccag
      421 cgagccgaag ccgacgtggc cggcggtgtc gagcagccgc cgggtcagct tgtgcaactc
      481 catgtcctgg tgggtcacgg caagggcagt gctgcccgtg tcgacgcgcc actgggtgat
      541 cttccgcccg ccttgggctg ctagctcttc gccgttgcgg ttgcggtagg tcaggcagaa
      601 gtagacgtcg ctgtctcggc ccggaatcca ctcctgcacg acgaagcggt cagcggcatc
      661 gaacggagcg aacggaagcc ggtcgaaatc gtcggcggtg tggagccggt aggccgtgcc
      721 ccacctactg aagtcctgcc attgtggtgt ccgctcgacc ggcttgaata ccacaggaaa
      781 ggtcatctcg cgcagggctg cgcgcaactg gccaggggtg gtgactatcg ctgtgtgggg
      841 gactgggaag tcgttggcca gtgcccactc atggaaaacc gacttgtcca gcaatcgatc
      901 aacggtcggc aggtcgggca gcacgaagtc gaactgcgtg gccagttcgg cggcgtgctc
      961 ggcgacgatg cgcaccacaa tgtcgtcggc agggaacagc accatcgggc cataccgcgc
     1021 gtgagccgca cgcagcatgg ccagccatgc ttccgtggtg ggcgcgctga ccggcaagac
     1081 gctgtgccat aacttggagc gacaggtcgg cgccgacggg gtgagggagg cgcccacgat
     1141 ggggaccccc gtgtcgcgcg ccccccgagc gatggctagc ccaatggcgg tcccggcacc
     1201 tgcgatcagc accggcggca gcaacgcctt cagatgccct ggggtgcttt ggtcgacgtc
     1261 agtatcgttc tcggtgaaca atcggaatcc cccctatacg caggacagcc gctgcagcct
     1321 ccccagcctt ggcggttgac cacgcccctt gttgccccct atcgagtggt ccggagggtc
     1381 cactccaacg gatccccggg ggatcctgta ctgcttgtca tcggcacttc gtctgaaagt
     1441 gttacacgag tacatagcgt taccatgacg ggtggggcgt acgtagggcg atcgggccgg
     1501 agcggctgct cgatccgcgg aggctggcca tcgttgggac agccacggta gccatgctgc
     1561 cggcgtcggc gttcgttttc tggtggtgca gcaccaggtg cacgaccggt tgccacagct
     1621 cgcggtcggc ttcgtgacgg gcgtggtggc actggcttgc ttcgggctgg ccttggcac
//
LOCUS       DATADN010001129         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1052016, whole genome shotgun sequence.
ACCESSION   DATADN010001129 DATADN010000000
VERSION     DATADN010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052016"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..211
                     /locus_tag="VHH53_14485"
                     /pseudo
     CDS             17..211
                     /locus_tag="VHH53_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            386..1321
                     /locus_tag="VHH53_14490"
     CDS             386..1321
                     /locus_tag="VHH53_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005442390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2291362.1"
                     /translation="MIEAHDLTKRYGTKAAVNGISFIIEPGTVTGFLGPNGAGKSTTM
                     RMIVGLDRPTTGTVTVNGKPYAQHRSPLREVGVLLDAKAVHPGRSARAHLRTMATTHG
                     IPASRVDEVIELTGLGAVAKKRVGGFSLGMGQRLGIAAALLGDPRTLILDEPITGLDP
                     EGVKWVRTLVRHLAGQGRTVFLSSHLMSEMAQTADQLLVIGKGRIITTGPVQDVIDSA
                     TAATVRVHSPQASVLAELVQGAGATVTSIDHGALEIRGLTAKQVGETAAAHGIVLHEL
                     SPQRASLEAAFMSLTQDAVEYRSETTLNAAEGEIL"
     gene            1318..>1666
                     /locus_tag="VHH53_14495"
     CDS             1318..>1666
                     /locus_tag="VHH53_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291363.1"
                     /translation="MTTEAAAPSHSIGDRPPRTVDAHLAFGGVLRAELLKLLSLRSTG
                     WTLGLTVVVMTLFGLLQAYSLRLPAEETESAGIAAPTHGAEVVIAGYQMGAVTIAVLG
                     ALMITPEYSTGMIR"
BASE COUNT          279 a          604 c          539 g          244 t
ORIGIN      
        1 ccgcaaggga ggagccatga ccgaaacggt ggtgaccacg ggtcccgccc ggcgccctca
       61 cctgcagcgt tggacgatcc tagggttgct cggagtcgcc cagctgatgc tgatcctgga
      121 catcaccgtc gtggccatcg ccttgccgca catgggtgcc gacctggggc tggggcgcga
      181 gaccctcagc tgggtggtga gcggctacac cgccggggca agcgcggacg gcctcacgcg
      241 cggcttcacc agcggtgcgg tcgccgctgc agccgccgcc ctgatggcgc tggtcctcac
      301 gcccggccac cgcgcgacat gaaccggctg tcccgccaca acgcgcccgc ggggtgcgaa
      361 ccggcctcgg cagaaaggtc cccgaatgat cgaggcacac gacctcacca agcgctacgg
      421 caccaaggcc gccgtgaacg gcatcagctt catcatcgaa ccgggcacgg tcaccggttt
      481 ccttggaccc aacggggccg ggaaatccac caccatgcgc atgattgtcg gcctggaccg
      541 gcccacgact gggaccgtga cggtcaacgg caagccctac gcccagcacc gctcaccgtt
      601 acgcgaggtc ggggtgctgc tggacgccaa ggccgtccac cccgggcgta gcgcccgtgc
      661 ccacctacgc acgatggcca ccacccacgg catccctgcc agccgagtgg atgaggtcat
      721 cgagttgacc ggtctggggg ccgtcgccaa gaagcgggtg ggtggcttct ccctgggaat
      781 gggccagcgc ctgggaatcg ccgccgcgct gctgggcgac ccgcgcacct tgatcctgga
      841 cgagccgatc accggccttg accccgaggg cgtgaagtgg gtgcgcaccc tggtgcgcca
      901 cctcgccggg cagggccgca cggtcttcct ctcctcgcac ctgatgagcg agatggccca
      961 gaccgccgac cagctgctgg tgatcggcaa gggtcggatc atcaccaccg gcccggtcca
     1021 agacgtcatc gactctgcca ccgccgccac ggtgcgggtc cacagcccgc aggcgagcgt
     1081 gctggccgaa ctcgtccaag gcgccggtgc caccgtgacc tcgattgacc acggcgctct
     1141 ggagatacgc ggcctgaccg ccaaacaggt cggcgagacc gccgccgccc acggcatcgt
     1201 cctgcatgaa ctgagcccgc agcgggcgag cctggaggca gccttcatga gcctcaccca
     1261 ggacgccgtg gagtaccgct ccgagaccac cctcaacgcc gcagagggcg aaatcctgtg
     1321 actactgaag ccgctgctcc aagccactcg atcggtgacc gcccgccccg gacggttgac
     1381 gcccacctcg ccttcggcgg ggtgctgcgg gcagagttgc tcaagctcct ctcgctacgc
     1441 tcgacgggat ggacgttggg tctcacggtc gttgtcatga cgcttttcgg cctgctccag
     1501 gcctactcat tgcgccttcc agccgaggag accgagagcg ccgggatcgc tgcccccaca
     1561 cacggtgccg aggtggtcat cgccggatac cagatgggtg cggtaaccat cgccgtgctc
     1621 ggggcgctga tgatcacccc ggaatactcc acaggaatga tccgct
//
LOCUS       DATADN010001130         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1202847, whole genome shotgun sequence.
ACCESSION   DATADN010001130 DATADN010000000
VERSION     DATADN010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202847"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..352
                     /locus_tag="VHH53_14500"
     CDS             212..352
                     /locus_tag="VHH53_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291364.1"
                     /translation="MSTGQDHEPGLYQIRVRGHLESRWAAWFDGMSLSYETDGTTSAT
                     HR"
     gene            complement(1528..1695)
                     /locus_tag="VHH53_14505"
     CDS             complement(1528..1695)
                     /locus_tag="VHH53_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291365.1"
                     /translation="MSTEQQHPDAWTEHQAATKRTSEEALAKRAEERADIEEDRHRQQ
                     ELDDAEMGGES"
     gene            complement(1993..>2220)
                     /locus_tag="VHH53_14510"
     CDS             complement(1993..>2220)
                     /locus_tag="VHH53_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291366.1"
                     /translation="GDLDDVLARAAAADQPPPLGPFRAYQVLTADLADLTGLDLTQTI
                     ALDLYRARTAGERPQATRPWRRLTALSDIQL"
BASE COUNT          343 a          719 c          766 g          392 t
ORIGIN      
        1 tctacgccaa gctcggtgtg accaaccgcc gcgccgctgt gcgccgtggc gacgaactcg
       61 acctgacgcc ccgccgcccc gccgtccacc agcggtagcg ccgaccgccg gagcggcggg
      121 aaatcaccac gtcgatcacc acacctggtg atccggtctc atcacatccg ctcctacctt
      181 tcgctcgcac cgcggaagga gatgagccga cgtgtcgaca ggccaggacc acgagcccgg
      241 gctgtaccag atccgcgtca gagggcacct cgagtcccgc tgggccgcct ggttcgacgg
      301 gatgagcctc agctacgaga ccgacggcac cacgtccgcg acgcatcggt gacctccgcg
      361 ggccggggga ccgcagcttg gcccagtccg gcagctcgcc ggtgcccagc gggagctgca
      421 gctgggcgca aagtcccgcc atcgcccgcc ccgggtgcgg gtacggcagc gcgctggtgg
      481 ccgtgtacgt ccggaacacg acggtgatcg tcctcgccac ctgctggcct gatcggtcag
      541 cggacacagg tgtcacccag cgggatgacc gcggcggcgc gctactcgca gacggggagg
      601 ccgtccgggc tccagtcttc cgggggagca gctcactcgg tacccggcgg cctggtgcgt
      661 ccggccgaca acaaattcag ggagagccga ccggtggtac agccgctggc ggctgaaaaa
      721 gactcagtta ccgatagagc accttgcccc cgcagtggga gcagtactcg gatgaggcag
      781 ttgctatttg gctcccgcaa ctacagcagt aaggctctgt gttgtcctga gcattccctg
      841 agcgtgctac gccacacacg gagcagtact tggacgttac ggtcgaatat tcttcgccgc
      901 agccggggca gtaaccgggt cgtagcgcag tcgggatata cggacgatcg gttccgggct
      961 tgccacattg aggacagaat cttgtgaagc ctgatttatt tccacactcg tcacagtatg
     1021 tgtgccgtat ctctcccatg atgcatggta gctggaccgt caagaatcag gacccgtggt
     1081 ggttcctccc cgtcagagca cgctggcggt ttgtgctctg tgacatccat acccctccag
     1141 gcctgcgatg gaagcacttt cgtgcggcgt tctactccaa gatgtcaggc caccgtcggc
     1201 caccgacctg cgcacgacta ccccgctctt tcgtgatctt gctgaggtcg gtcatcgagc
     1261 gggtcgtgac cgccgcggcg gtgatctggg tccgggtggg acagatcgcc ccgtggcgtc
     1321 ggggcccagt agggtccgcg gtgcggctcc agccggacca cggtcatgac acagccagcc
     1381 ccgtccgcgt cgtcgacgca tgccgtgtcg gaccgcgtca tcggcccgta ggcgctccct
     1441 ttgccggcgt ccgcgtcatt cgctcagccg gcgcggcggg gctggcgcgc cttggttgac
     1501 aaccggggag gggcgacggc attgcattca ggattccccg cccatctccg catcgtccaa
     1561 ttcctgctgc cgatgccggt cctcctcgat gtcggcgcgc tcctccgccc tcttggcgag
     1621 ggcctcctcc gaggtccgct tcgtcgcggc ctgatgctcg gtccaggcgt cggggtgttg
     1681 ctgctcagtg ctcatggtgg ggtgcccttt cggaaaaggg gggcatcctc cgagacaggg
     1741 atccgtcggg gccctattgc tggggccggt gaggacggcg actctcgacg ggccgacgat
     1801 ccgactttgc cctgttcggg tcaagaggga ctgacaggtc agctcggatc gtgaggcgcg
     1861 gctgcgtggg aacataagca tgaacgacgg tggccttgcg agtgtgactg gggtaggggg
     1921 cgcacgaggc cagcaggtcg agcgccgatg cgccttgctg catatctgcg cctggccctg
     1981 aggacattgg cgtcacagct ggatgtcgct gagtgcggtc agcctgcgcc acgggcgagt
     2041 ggcttgaggg cgctcaccag cggtgcgggc gcggtacagg tcgagggcga tggtctgggt
     2101 gagatcgagg ccggtgaggt cggccaggtc ggcggtgagc acctgatagg cgcggaacgg
     2161 tcccagtggc ggtggctggt cggcggccgc agctcgggcg aggacgtcgt cgaggtcgcc
//
LOCUS       DATADN010001131         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_882031, whole genome shotgun sequence.
ACCESSION   DATADN010001131 DATADN010000000
VERSION     DATADN010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882031"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1024)
                     /locus_tag="VHH53_14515"
     CDS             complement(101..1024)
                     /locus_tag="VHH53_14515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2291367.1"
                     /translation="MRILVVDDEPLNLRLVEAAVTRLGHQATAAEDGEAAWQCYNQDK
                     PEVLITDLLMPRVEGLELCRRIRADAGAGYTYIILVTALSDRRDVARGMEAGADDYLI
                     KPVDLFDLEMRLIAAQRVTDLHAQLARHRAQLAHLARHDPLTGLGNRRSLDEDLETLH
                     ARSRRYGRGYALAMCDIDWFKAYNDTYGHQAGDQALRAVTATIARELRGGDSVYRYGG
                     EEFVLVLPEQTPDTALVAVERVRSAVERLAIPQPGAGPDGVLTVSAGITAYAPGDQTT
                     AEELLRQADAALYRAKSAGRNRIAVADNLPS"
     gene            complement(1026..1649)
                     /locus_tag="VHH53_14520"
     CDS             complement(1026..1649)
                     /locus_tag="VHH53_14520"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX2291368.1"
                     /translation="MRQRFGCWVVVAVLAAGCATPNSNQPTASDQADILFMQHMVPHL
                     LQTTSMVDLARDRITRPELARLADSIDRRGQAHLQQLQGWLNERGLAPYDPQQQPGSG
                     KETDLERLARIRGARFDLEFLTVMTARHRAGSKLAATEVREGSVPEVRELAQQLLAEH
                     QDQIGKMQAWRRAWAKADANHSTARASAGAVDHRWQHFAARRAARRA"
     gene            1867..>2196
                     /locus_tag="VHH53_14525"
     CDS             1867..>2196
                     /locus_tag="VHH53_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291369.1"
                     /translation="MRRFSFSVLLVAICLLPVGIGSGTSAHSHAKAALDGALANTASA
                     QAQVLEDYFSRARSIDLLTAKNPAFRHFYRMPGDRTAKIRAGGPVLDQANEALGNLEE
                     LFPDSIGE"
BASE COUNT          308 a          743 c          754 g          391 t
ORIGIN      
        1 cgcgcagggc ggagggatgt tcctccgccc tgcgcgttcc ctggctgagc tgcgatgctc
       61 gcgagcctgt cgatgatggt gcagccgtga cgacggttcg ctagcttggc aggttgtccg
      121 ccacagcgat gcggttgcga ccagccgact tggcccggta gagggccgcg tcggcctgtc
      181 tcagcagctc ctcggctgtg gtttggtcgc caggagcata ggccgtgatc ccggcgctca
      241 ccgtcagcac gccatcgggg ccagcgcctg gctgggggat ggcgagccgc tccaccgcgc
      301 tccgcacccg ctctaccgcc accagcgccg tgtctggggt ctgttcgggc agcaccagca
      361 caaactcctc cccgccgtag cggtagacac tgtcaccccc tcggagctcc cgggcgatgg
      421 tcgcggtcac cgcccgcagc gcctggtcgc cggcctggtg cccgtaggtg tcgttgtagg
      481 ccttgaacca gtcgatgtcg cacatggcca gggcatagcc gcgaccgtag cgtcggctgc
      541 gggcgtggag ggtctccagg tcctcgtcca gcgaccgccg gttgcccagg ccggtcagtg
      601 gatcgtgacg ggccaggtga gccagttgtg cgcggtggcg ggccagctgg gcgtgcaggt
      661 cagtcacccg ctgggccgcg atgagcctca tctccaggtc gaacagatcg accggcttga
      721 tcaggtagtc gtcggcgccg gcctccatgc ctcgcgccac gtcccggcgg tcgctcaggg
      781 cggtgaccag gatgatgtag gtgtagccgg cgcccgcgtc ggcccggatg cggcggcaca
      841 actccagccc ctcgaccctg ggcatcagca gatcggtgat gagtacctca ggcttgtcct
      901 ggttgtagca ctgccaggcc gcctcgccat cctcggcagc cgttgcttgg tgtcccaagc
      961 gcgtgaccgc ggcctcgacc agacgcaggt tgaggggctc gtcgtccacc accaagatcc
     1021 tcatctcacg cccttcgagc agcgcggcgg gccgcgaagt gttgccagcg gtggtccacg
     1081 gcaccagcgg aggcccgggc cgtcgagtgg ttggcgtcgg ccttggccca ggcgcgccgc
     1141 cacgcctgca tcttgccgat ctggtcctgg tgttcggcca acagttgctg ggcgagctcg
     1201 cggacctcgg gcacgctgcc ctcgcggacc tcggtagcgg cgagcttgct gccggcgcgg
     1261 tgccgtgccg tcatcaccgt gaggaactcc agatcgaatc tggcgccgcg aattcgagcc
     1321 agccgctcca ggtcggtttc cttgccgctg ccgggctgct gttgcgggtc gtagggggcc
     1381 agtcctcgct cgttcagcca gccttgcagc tgctggaggt gcgcttgtcc ccgtcggtcg
     1441 atgctgtcgg ccagccgggc cagctcgggg cgggtgatcc ggtcgcgggc caggtctacc
     1501 atcgaggttg tctgcagcag gtgggggacc atgtgctgca tgaacaggat atcggcctgg
     1561 tcggaggccg tgggctggtt tgagttaggc gtggcgcagc cggccgccaa caccgcgacc
     1621 acaacccagc atccgaaccg ttgtcgcact atcgcccctt ggccgtcgtg tgcaagcagc
     1681 tggccgccgc gatcggtcag ttaccagcta ctagagatgt cgacccagcg gctggcggct
     1741 tgaccggacc accacagaat ccgagccctc atgttggtgg acttgaagtc ctaagacgcc
     1801 gggcggtggc tgccgatgga actcccatca cgtcagttac gtcgagtgtc gagttaggac
     1861 ctggggatgc gtcgcttctc gttctctgtg ctattggtag ccatctgcct gctcccggtc
     1921 gggatcggca gcggcacctc ggcccacagc catgccaagg ccgccctgga cggggccttg
     1981 gccaacaccg ccagcgccca ggcccaggtg ctggaggact acttctcccg ggcccggtcg
     2041 atcgacctgc tgacagccaa gaaccccgcc tttcggcatt tttaccggat gccgggtgac
     2101 cgcacggcga agatccgcgc tggcgggcca gtcctggacc aggccaacga ggccctgggc
     2161 aatctggagg agctgttccc cgacagcatc ggggag
//
LOCUS       DATADN010001132         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_742009, whole genome shotgun sequence.
ACCESSION   DATADN010001132 DATADN010000000
VERSION     DATADN010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742009"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VHH53_14530"
     CDS             complement(<1..538)
                     /locus_tag="VHH53_14530"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291370.1"
                     /translation="MTTSGDGREESTAEVLRIVPEAGDTVQDGVLREFTDPGGTLYQA
                     DSPRAAIDRFMAQRGGDLKAGGADLRELDSAEGAGTLRVRYGQFHNGLPVLGATLQAV
                     ASTRTSSVVKMSNAADQDMSGAPAPEQAPPIDELRTAVLAPFEAELDSAEIVRSQLAY
                     LRNVERPPVPEADYPTASV"
     gene            1158..1553
                     /locus_tag="VHH53_14535"
     CDS             1158..1553
                     /locus_tag="VHH53_14535"
                     /inference="COORDINATES: protein motif:HMM:NF025000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2291371.1"
                     /translation="MVINQLPTNLVHLRHGVIHDVLARWFGVSRSTIARAVGQVRPLL
                     AERGCTVDGGLRLRTLADVNTHPGASGRVGLLDATEVPVRRPAAHRAGPQRFASGKAR
                     ANTVKALVLTDPEGDCCSADRPAQAPSTT"
     gene            1704..1952
                     /locus_tag="VHH53_14540"
     CDS             1704..1952
                     /locus_tag="VHH53_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291372.1"
                     /translation="MLPAVAAAHDAERRAHAWQRTRVEHGISHLKNWRALTRHLGRRE
                     HLDTMLPAVAGLVSSQERAPRPDQLHRQLKALPAGTAA"
BASE COUNT          308 a          768 c          694 g          313 t
ORIGIN      
        1 ccaccgacgc cgtcggatag tcggcctcgg gtaccggggg ccgttcgacg ttccggagat
       61 aggccagctg gctgcgtacg atttcagcgg agtctaactc ggcctcgaac ggcgcgagca
      121 ccgcggtcct cagttcgtcg atcgggggcg cctgttcagg cgccggtgca ccgctcatgt
      181 cctggtcggc ggcattgctc atcttgacga cactgctggt gcgcgtgctc gccaccgcct
      241 gcagggttgc tcccagcacc ggaaggccgt tgtggaattg gccgtagcgc acccgcagtg
      301 tgccggcgcc ctcagcggaa tccaactcac gcaggtcggc cccaccggcc ttcaggtctc
      361 caccgcgctg cgccatgaag cggtcgatgg ccgcccgtgg gctatccgct tgatataacg
      421 tgccgccggg gtcggtgaac tcccgcaaca ccccgtcctg cacggtatcc ccagcctcgg
      481 gcacgatgcg caagacctcc gcagtcgatt cctcacgtcc gtctccgctg gtggtcacct
      541 gcacccctcc ctctacgagt gatcccctgc agtgcggcaa tcccctaccg ggtggacgct
      601 cgccatgaac atccacccag atgtgaagcg cggcgcgcgt tggcgccgtc caacaggtga
      661 gtgtctaccg gtcgagcgag ccactgctcc tggaccagca attcgggatt ctcgggcgac
      721 actgagctcc acatggatgt cgagcctcat ctcgcgctcc tcccaccaca tgtcgggtcg
      781 cgaagaatgg cgcaggcgtc gcgccggcaa aggtacggag gtctccctct tggtgcctgc
      841 gaggagccac agtcacacta ttcggaggca cgtcacggcg ccgtcaccgc tgcgtcaagg
      901 ccgagccagt cctcgacgac tgtccgtaac gaccgctcgc ctggtccgcg ggatgtcgtg
      961 ctcctgaggg accgggcagg gtcgtggtgt gtcagagacg ctggcccgtg ccgcggctgt
     1021 cgccgatggc cggatgaccg ggctgtcccg ggaggtctcg gcggagctgg tcgccgcgct
     1081 ggcaggcgtg gcaggacgcg aggtcggccg atcggccgcg ccagcgggcg gtcggagccg
     1141 gtgcccggca ccggctggtg gtcatcaacc agctgccgac caacctggtg cacctgcgcc
     1201 acggggtcat ccacgacgtc ctggctcgct ggttcggggt ctcccgctcg acgatcgccc
     1261 gcgcggtcgg gcaggtccgc ccgctgctgg ccgagcgcgg ctgcaccgtc gacggcgggc
     1321 tccggctgcg cacactggcc gacgtgaaca cccatccggg cgccagcggg cgggtcggcc
     1381 tgctcgatgc caccgaggtg ccggtgcgcc gcccggcggc gcaccgggcc ggcccgcagc
     1441 ggttcgcctc gggcaaggcc cgcgccaaca ccgtcaaggc gctggtcctc accgaccctg
     1501 agggggactg ctgttctgcg gacagacccg cgcaggctcc atccacgacc tgacccgggt
     1561 ccgccagtcc ggcctggtgg aactgctggc cctcacctcc ggcctcacgc tgctggccga
     1621 cgccggctac cagggtctga gcacccagac cgccggcgtg gtggtcaccc cgtggccggc
     1681 tcggcggaag aacccaggtg ccggtgttgc ccgcggtcgc cgcggcgcac gacgccgaac
     1741 gccgggccca cgcgtggcag cggacccgcg tggagcacgg catcagccac ctgaagaact
     1801 ggcgagccct cacgcgccac ctcggccgac gcgaacacct cgacacgatg ctgcccgcgg
     1861 tcgccggact ggtctccagc caagaacgag ccccacgacc cgaccagctc caccgccaac
     1921 tcaaggcgct cccggccggc accgccgcgt gatcaacctg gccccgaaca agtctgctac
     1981 tgcccgcggc taaccatgca cgaggtcgta agtgacatga ccacgtcggt cggtgtgcac
     2041 gaggccaaga cgcacctgtc gcggctgctg gagaaggtcg ccg
//
LOCUS       DATADN010001133         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1052324, whole genome shotgun sequence.
ACCESSION   DATADN010001133 DATADN010000000
VERSION     DATADN010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052324"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1594)
                     /locus_tag="VHH53_14545"
     CDS             complement(<1..>1594)
                     /locus_tag="VHH53_14545"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX2291373.1"
                     /translation="ESAQQRWLRSGDIAHRVEPDLKNGHGGLRDVQLLDALSAGQLLD
                     RAGPEVCAARDLLLDVRTELHRLAGRSRDVLRAQDADEVAAALGLGDRFDLARSLSGA
                     ARTVVYAMEVGLRAARAALPPRGRARLTGLRTLGRGPVRRPLDEGVVEHGGEVALARD
                     IHPARDPALALRVAATAARTGMPVAAGTLTTLADTAPELRRPWPHPALDGLLSLLGTG
                     QPAVDVIEAMDRTGLWGRLFPEWGAVRDLPPRDRVHVLTVDRHLVETAAQAARLTTTV
                     ARPDLLLLGALLHDLGKGRGGDHSIVGAALAEQIGHRLGLPHSDVAILCAMVRHHLLL
                     PHTATRRDLEDPTTTRRVVDTLGGDLILLELLVALAEADSLATGPGVWTEWRAALISD
                     LAERCRAVLAGRPVTALEPLSEEHRALAEQVAASGALHVTATVASKSSPAAQGAATTI
                     TVVTSGRPSVLALATGVLALHSLQVHSADIRIVELPAGDSVAVDTFTVSPRFGRLPDV
                     ALLRQDLVRALDGSLKLAERLVA"
BASE COUNT          259 a          592 c          536 g          207 t
ORIGIN      
        1 ggcgaccagc cgttccgcca atttcaacga gccgtccagc gcccgcacca ggtcctggcg
       61 cagcagcgcg acgtcgggca agcgcccgaa gcgtggcgag acggtgaagg tgtccaccgc
      121 cacggagtct ccggcgggga gttcgacaat ccggatgtct gcggaatgga cctgcagcga
      181 gtgcagcgca agcacccctg tggccaatgc cagcacgctg ggacggccag aagtcaccac
      241 cgtgattgtc gtggccgcac cctgcgctgc aggggaggat ttcgatgcca cggtcgccgt
      301 cacgtgcagc gcgccgcttg cggcaacctg ctcggccagc gcacggtgct cctcggagag
      361 cggctccagt gctgtcaccg gtcgaccggc cagtaccgcg cggcaacgct cggccaggtc
      421 ggagatcaac gcggcccgcc attcggtcca cactccagga ccggtggcca aactgtcagc
      481 ctcggccaaa gcgaccagca gctccagcag gatcaggtct ccaccgaggg tgtcgaccac
      541 ccgccgcgtg gtcgtggggt cctcgaggtc ccggcgggtc gcggtgtggg gcagcagcag
      601 atggtggcgc accatcgcac agagaatcgc gacgtccgag tgcggtagcc cgagccggtg
      661 cccgatctgc tcggccaacg cggcgccgac gatggaatgg tcaccgccac gtcccttgcc
      721 caaatcgtgc agcagggcgc cgagtagcag cagatccggg cgggcgaccg tggtggtcag
      781 ccgcgccgcc tgtgccgcgg tctccactag atgccggtcc acggtcaaga cgtgcacccg
      841 atctcgcggt ggcaggtcac ggaccgcacc ccactccggg aacagccgcc cccacagccc
      901 ggtgcgatcc atggcttcga tgacatctac cgccggctgg ccggtgccca gcaacgacag
      961 caacccatcc aacgccgggt gcggccaggg ccgacgtagc tccggggccg tgtcggcaag
     1021 ggtcgtgagg gtgccggcag cgaccggcat gccggttcgc gctgcggtcg ccgcaacccg
     1081 caacgccagc gccggatccc gcgccgggtg gatgtcgcga gccagcgcca cctcaccacc
     1141 gtgctccacc accccctcgt ccaacgggcg gcgtaccggt ccccgcccga gggtccgcaa
     1201 cccggtcagc cgggcccggc cccgcggggg cagcgcggcg cgtgcagcac gcaacccgac
     1261 ctccatcgca tagaccacgg tccgggccgc tccggacaga cttctagcca agtcgaaccg
     1321 gtcaccgaga cccagggcgg cggcgacctc gtcagcgtcc tgtgcccgca gcacgtcgcg
     1381 acttcggccg gcgagccgat gcagctcggt gcgcacatcc aggagaagat cgcgcgcggc
     1441 gcacacctcg ggtccggccc gatccaggag ctgccccgcg gataacgcat cgagcagctg
     1501 aacatctcgc agcccgccgt ggccgttctt gaggtctggt tccacccggt gcgcgatgtc
     1561 cccgctgcgc agccagcgtt gctgcgccga ctcg
//
LOCUS       DATADN010001134         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_596361, whole genome shotgun sequence.
ACCESSION   DATADN010001134 DATADN010000000
VERSION     DATADN010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596361"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1955
                     /locus_tag="VHH53_14550"
     CDS             <1..>1955
                     /locus_tag="VHH53_14550"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX2291374.1"
                     /translation="GGTTTRTAGTARTASGGPRTSGTASGTSGTGTGTGTGTGGTGTG
                     GTGGTGAGTAGTAGTASSTASGASGTSGLDSQLTGLSDALSDLPPAVGVNGDGIGSNS
                     WVVSGKHTITGKPLLANDPHLSPSLPSVWYQMGLHCRSVSSACQYDVSGYTFAGMPGV
                     IIGHNQDIAWGMTNSGVDVTDLYLEKITGNGYLYDGKVKPFETREETIKVAGGSPRKI
                     VVRETNNGPLLSDRAKELVKVGKKATVDTAAPDRGDGYGVALRWTALQPGTSMDAVFA
                     MDRAKNWTDFRKAAALFDVPSQNLIYADTEDHIGYTLPGRIPKRSQDDDGSVPAPGWD
                     PKYRWTGYIQQDELPYEYNPERGYIVTANQAVIDPDKYPYTLTEDWGYGTRSQRITDL
                     IQSKIKDGGKISTDDMRQMQLDNSSEIARLLVPTLLKIDPENKDVREAQKLLEGWDYT
                     QDTDSAAAAYFNAVWRNILKLAFGNKLPKELRVKGQCLWVEPVNTTGPADEAEKVREC
                     GERDPDQAQPDGGDRWFEVVRKLMDQPDSDWWKTPKAGTRPAAVNRDQLFRRALIDAR
                     WELTAKLGKDIDTWTWGRLHRLFLKNQTLGTEGPGFVQYALNRGPWELSGGEATVNAT
                     GWNAAGGYGVVWVPSMRMVVNLGDLDKS"
BASE COUNT          363 a          723 c          642 g          227 t
ORIGIN      
        1 ggcggtacca ccacccgcac cgcgggcacc gcgcgcacgg cgtccggtgg cccccgtacc
       61 tcgggcaccg cctccggcac gagcggcacc ggcaccggca cgggcacagg cactggcggc
      121 acgggcacag gcggaaccgg cggcaccggg gccggcacgg cgggcaccgc gggcacggcc
      181 tccagcacgg cttccggcgc ctccggcacc tccggcctgg acagccagct caccggcctc
      241 agcgatgccc tgagcgacct cccgccggcc gtcggcgtga acggcgacgg catcggctcc
      301 aactcctggg tcgtcagcgg gaagcacacc atcaccggca agccgctgct ggccaacgac
      361 ccgcacctgt cgccctcgct gccgtccgtc tggtaccaga tgggcctgca ctgccgcagc
      421 gtctccagcg cgtgccagta cgacgtcagc ggctacacct tcgccggcat gccgggggtg
      481 ataatcggtc acaaccagga catcgcctgg ggcatgacca actccggcgt cgacgtcacg
      541 gacctctacc tggagaagat caccggcaac ggctacctgt acgacggcaa ggtcaagccc
      601 ttcgagacgc gcgaggagac catcaaggtc gcgggcggct cgcccaggaa gatcgtcgtc
      661 cgcgagacca acaacgggcc cctgctgtcc gaccgggcca aggagctcgt gaaggtcggc
      721 aagaaggcca ccgtcgacac cgccgcaccc gacagaggtg acggctacgg cgtcgccctg
      781 cgctggaccg cgcttcagcc gggcaccagc atggacgccg tcttcgccat ggacagggcg
      841 aagaactgga ccgacttccg caaggccgcc gcgctgttcg acgtgccctc gcagaacctg
      901 atctacgcgg acaccgagga ccacatcggc tacaccctgc cgggcaggat ccccaagcgc
      961 tcccaggacg acgacggctc cgtcccggcg cccggctggg accccaagta ccgctggacc
     1021 ggttacatcc agcaggacga gctgccgtac gagtacaacc cggagcgcgg ctacatcgtc
     1081 accgccaacc aggccgtgat cgacccggac aagtacccct acacgctcac cgaggactgg
     1141 ggctacggca cgcgcagcca gcggatcacc gacctgatcc agtcgaagat caaggacggc
     1201 ggcaagatct ccacggacga catgcgccag atgcagctgg acaacagcag cgagatcgcc
     1261 cggctgctgg tgcccacgct gctcaagatc gaccccgaga acaaggacgt ccgcgaggcg
     1321 cagaagctgc tggagggctg ggactacacc caggacaccg attcggccgc cgccgcctac
     1381 ttcaacgcgg tctggcgcaa catcctcaag ctcgccttcg gcaacaagct gcccaaggag
     1441 ctgcgggtca agggccagtg cctgtgggtg gagccggtca acaccaccgg gcccgccgac
     1501 gaggcggaga aggtgcgcga gtgcggcgag cgcgaccccg accaggcgca gcccgacggc
     1561 ggtgaccgct ggttcgaggt ggtccgcaag ctgatggacc agccggacag cgactggtgg
     1621 aagacgccca aggcgggcac ccgccccgcc gccgtcaacc gcgaccagct gttccggcgc
     1681 gcgctgatcg acgcccgctg ggagctgacc gccaagctcg gcaaggacat cgacacctgg
     1741 acctggggcc ggctgcaccg cctgttcctg aagaaccaga ccctcggcac cgagggcccc
     1801 ggttttgtcc agtacgccct caaccgcggc ccctgggagc tcagcggcgg cgaggccacc
     1861 gtcaacgcga ccggctggaa cgcggccggc ggctacggcg tggtgtgggt gccgtccatg
     1921 cggatggtcg tgaacctcgg cgacctcgac aagtc
//
LOCUS       DATADN010001135         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_449849, whole genome shotgun sequence.
ACCESSION   DATADN010001135 DATADN010000000
VERSION     DATADN010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449849"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..264
                     /locus_tag="VHH53_14555"
     tRNA            170..264
                     /locus_tag="VHH53_14555"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Thr,seq:tgt)
     gene            501..1835
                     /locus_tag="VHH53_14560"
     CDS             501..1835
                     /locus_tag="VHH53_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291375.1"
                     /translation="MDGDRRPGRHDAGEATPQTALRVAKPTRVRLAWALGALAVIVVV
                     ATLLLILQARGAQLPPGFRTAGIVETLQFLGPAIVGAVLAARRPANPIGWLLLGLGLC
                     FALYPLVVMYVTTALVVVPGGLPGVRWVAWVGNWIWVPAHGCIALLFLLFPNGRLLSP
                     RWRPVAWSALGAAAVLAIAAACYPGPLEVGRLTPDTEVLPGFDNPLGVAAFGPSLPVM
                     LVVIQAVAVLGIVSLALRFRRSRGEERQQLKWVAYAGLFYGLQTVVLVVGNASGLLSQ
                     EGMPWLDVLDYATAFGLLLAIAVAVLKYRLYDIDRIINRTLVYGLLTAALGLCYVAGS
                     LLFVLVAGASADPPSWLVAATTLAAAAVFRPARRRIQATVDRRFNRRRYHAARTIEAF
                     SARLRDEVDLDTLSAELLAVVDRTMEPTRASLWLRPPMERSGHAIDAYASIR"
BASE COUNT          256 a          650 c          688 g          337 t
ORIGIN      
        1 cgcagcctgg ttagcggcga gcctcgccca gcagcgagtg tggtgccgca aggctgggac
       61 gtcaggctga ggggcggtgg gacgatgcgg gatgtcattc tgcttgtcat tccacccggc
      121 gttactcggc ccgacgggag cgcactggct ggacgaccca cttgacctga gctgcaagga
      181 gagcactggc cagcacgccg tggacaatcc gctcctgtct tgtaaacagc aggttggggg
      241 ttcgagtccc cccgccagct cccaacacgg cagggcccgg tcggtgctga agctggcccc
      301 gatcctgtgc ggcgcctgcg cccaaccctt cgagcccggg gacgacgggg ccggttgacg
      361 cgtacgaggc tgttgcggaa caccctccgt ttgaaggtgt gaggtggccg taacggtctc
      421 gggacggtcg gcgcggagca tgacggcgac gacctcaaga ggcagagtgg cggcgtcaga
      481 gcaaacggga ggcgccacag gtggacggcg acaggcggcc aggacgccac gacgccggcg
      541 aggcgacccc ccaaacggcc ctgcgcgtgg ccaaaccgac ccgtgtccgc cttgcctggg
      601 cgcttggagc gctcgcggtc atcgtcgtgg tggcgacgct gttgctgatc ctccaggcgc
      661 gcggggcgca actgccgccc ggcttccgaa cggccgggat cgtcgagacg ctgcagttcc
      721 tgggtccggc gatcgtcggt gccgtgctgg cggcacgccg cccggccaac ccgatcgggt
      781 ggctgctgct cgggctcggg ctgtgctttg ccctgtatcc gctggtggtc atgtacgtca
      841 ccacggcact ggttgtggtc ccgggtgggc tacctggtgt ccggtgggtc gcctgggtcg
      901 gcaactggat ctgggtgccg gcccacggct gcatcgcgct cttgttcctg ctgtttccca
      961 acggccggct gctgtcccca cgctggcggc ctgtcgcctg gagcgccctc ggcgcggccg
     1021 ccgtgctagc gatcgccgca gcgtgctacc ccggtccgct cgaagtgggc cgattgaccc
     1081 cggacaccga ggtcctccca gggttcgaca atcctctcgg ggttgccgcc ttcggcccga
     1141 gccttccggt catgctcgtg gtgatccagg cagtggcggt ccttggcatc gtctcgctgg
     1201 cgttgcgctt tcggcggtca cggggtgagg agcgccagca gctgaagtgg gtcgcctacg
     1261 ccgggttgtt ctacgggctc cagacggtgg ttctggtcgt tggaaacgcc tctgggctgc
     1321 tgtcccagga gggaatgccc tggctggacg tgctcgacta tgcgaccgcg tttggactgc
     1381 tgctggccat cgcggtcgcg gtgctgaagt accggctgta cgacatcgac cggatcatca
     1441 accgcacctt ggtctacggg ctgctcaccg cggccctcgg gttgtgctat gtggccggat
     1501 cgctgctgtt cgtgctggtg gccggcgcca gtgcggaccc gccgagctgg ctggtcgctg
     1561 ccacgaccct ggccgcggca gcggtgttcc gaccggcccg ccgccgcatc caggccaccg
     1621 tggaccggcg cttcaaccgg cgtcgctacc acgcggccag gacgatcgag gccttcagcg
     1681 cccgcctccg cgacgaagtg gacctggaca ccctctcggc cgagctgctt gctgtggtcg
     1741 accggaccat ggaacccacc agggcgtcgt tgtggctgcg accacccatg gagcgctcgg
     1801 ggcatgccat tgacgcgtac gcgtcaatta ggtagagcgc gaggttcctc cctggccggt
     1861 cgatggcgag cggcaggcta cgaccactgc cgctagagcg acgcggcagc gccctccgcg
     1921 cagcggcacc c
//
LOCUS       DATADN010001136         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_449948, whole genome shotgun sequence.
ACCESSION   DATADN010001136 DATADN010000000
VERSION     DATADN010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449948"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VHH53_14565"
     CDS             complement(<1..378)
                     /locus_tag="VHH53_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX2291376.1"
                     /translation="MTVPRKPSRPAKPGPPRRRSGPRRPPVEDLPRRAALEALAAVRE
                     RDAYVNLVLPGLLRQRGIRGRDAALATELCYGTCRAQGLLDAVLESCTDRPLSEVDGE
                     LLYALRLGSYQLLRTRVPSHAAVG"
     gene            complement(419..1360)
                     /gene="fmt"
                     /locus_tag="VHH53_14570"
     CDS             complement(419..1360)
                     /gene="fmt"
                     /locus_tag="VHH53_14570"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX2291377.1"
                     /translation="MRLVFAGTPEVAVPALQALLDSDRHEIVAVLTRPDAPAGRGQRP
                     ARSPVGALADKAGLQVLTPRRLTEPDFLAQFSALAPDCCPIVGYGALVPRLALDIPRH
                     GWVNLHFSLLPAWRGAAPVQAAIRHGDEITGASTFALEEGLDTGPVYGTVTEVIRPED
                     TAGELLSRLANAGAGLLVATVDGIADGILHPVAQPADGVSHAPKLTAEDAHVDFSAPA
                     FAVHRQVRAVTPAPGAWVRFRDQRVGLGPVQLADVHGPDAGGLGPGELRATKREVLVG
                     TASSAVRLGEVTAPGKRAMPAPDWARGARLGPGERFE"
     gene            complement(1364..1927)
                     /gene="def"
                     /locus_tag="VHH53_14575"
     CDS             complement(1364..1927)
                     /gene="def"
                     /locus_tag="VHH53_14575"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX2291378.1"
                     /translation="MAVQPIRLFGDPVLRARAAEVVDFDKELRNLVRDLLETMVQEGA
                     AGLAAPQLGVSLRVFTYRCDDVAGDDLTGHLVNPTFDVLGDEMQDGPEGCLSIPGLSW
                     DCRRHLRVVARGRNQHGEPIEVHGTERLARAIQHETDHLDGVLFIDRLDRELRKQAMR
                     EIRQARWFGNAEPVVKVSPHPILGKAR"
BASE COUNT          317 a          749 c          709 g          275 t
ORIGIN      
        1 gccgacggcg gcatgcgacg gtacccgggt ccgcagcagt tggtagctgc ccaaccgcag
       61 cgcatacagc agttcgccgt ccacctccga cagcggacga tcggtgcagg actccaacac
      121 cgcatccagc aggccctgcg cgcggcaggt tccatagcac agctcggtgg ccagcgcggc
      181 gtcccggccc ctgatcccgc gctgccggag caggccaggc agcaccaggt tgacgtaggc
      241 gtcacgctcg cggaccgcgg ccagggcctc cagcgcggcg cgccgaggga ggtcctccac
      301 cggtggccgg cgtggcccgg agcgccgtcg gggcggcccg ggcttggccg gtcgggatgg
      361 cttgcgaggg accgtcacgg cggaccttcg acaacctggg taatgttcac attcaccgtc
      421 actcgaatcg ctcccccggc cccagccgcg caccacgggc ccagtccggt gcgggcatcg
      481 cccgcttacc gggcgcagtg acctcaccca gccgcaccgc gctgctcgcc gtgccgacga
      541 gcacttctcg cttggttgcc cgtagctcac ccggaccgag cccgccggca tcaggcccgt
      601 gcacatcggc aagctgcacc ggcccgaggc ccacgcgttg gtcccgaaac cgaacccagg
      661 caccgggagc gggcgtcacc gcacgcacct ggcggtgcac cgcgaacgcc ggggcgctga
      721 agtccacatg cgcgtcctcg gcggtgagct tcggggcgtg cgagacacca tcggcgggct
      781 gggctaccgg atgcaggatg ccgtcggcga tcccgtccac agtggccacc aacaggccgg
      841 ccccggcgtt ggccaaccgg gacagcagct caccagcggt gtcctcgggg cggatcacct
      901 cggtgaccgt gccgtatacc gggccggtgt ccaggccctc ctccaacgcg aaggtggacg
      961 caccggtgat ctcatccccg tgccggatcg cggcctgcac cggcgcggca ccccgccagg
     1021 caggcagcag cgagaagtgc aggttcaccc agccatgccg ggggatgtcc agcgcgagcc
     1081 gcgggaccag cgcgccgtag ccgacgatcg ggcagcaatc cggggccagc gccgagaact
     1141 gcgcaaggaa gtccggttcg gtcaggcggc gcggggtgag cacctgcaac cccgctttgt
     1201 cggctaaggc gcccaccggg gaacgagccg gccgctgtcc gcgccctgct ggcgcgtccg
     1261 gtcgggtaag caccgcaacg atctcgtgcc ggtcggagtc cagcagcgcc tgcagcgcgg
     1321 gcactgccac ctcaggcgtg ccggcgaaga ctaaccgcac cgctcagcgc gccttgccga
     1381 gtatcggatg cgggctgact ttgaccacgg gctcggcgtt gccgaaccag cgtgcctgcc
     1441 ggatctctcg catggcctgc ttgcggagct cacggtcgag ccggtcgatg aacagcacgc
     1501 cgtcgaggtg gtccgtctcg tgctggatcg cgcgggccag ccgctcggtg ccgtgcactt
     1561 cgatcggctc gccgtgctgg ttgcgtccac gggcgacgac ccgcagatga cgccggcaat
     1621 cccacgacag gccggggatc gacaggcagc cttccggtcc gtcctgcatc tcatcaccca
     1681 gcacgtcgaa ggtgggattg accagatgcc cggtgagatc gtccccggcg acgtcgtcgc
     1741 agcggtaggt gaacacccgc agcgacacgc ccagctgggg cgcagcgagc ccggctgcac
     1801 cctcctggac catcgtctcc agcagatcgc ggacgagatt gcgcagctcc ttgtcgaagt
     1861 cgacgacctc ggccgcgcgg gcacgcagca ccgggtcgcc gaagagccgg atgggttgca
     1921 cagccaccag cgcagtatgc cttgtgcagt gcactacgcc agctcgactg ggtcgagctc
     1981 gacccgcagc ggatcgggta gcttgcgggc gctgcgtccg gcctgcagcg cggccaatgc
     2041 ggtggccagg
//
LOCUS       DATADN010001137         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1052937, whole genome shotgun sequence.
ACCESSION   DATADN010001137 DATADN010000000
VERSION     DATADN010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052937"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VHH53_14580"
     CDS             <1..185
                     /locus_tag="VHH53_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291379.1"
                     /translation="CTPPVLFAPARAPVLLSITTSGLVCLTHGPHSRHPTCHNTSSTV
                     PTTVRGLPSPGASAYA"
     gene            772..1047
                     /locus_tag="VHH53_14585"
     CDS             772..1047
                     /locus_tag="VHH53_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291380.1"
                     /translation="MSDALTLAEIDAQHVELLPARTVLSLFMTAGGGGARGGNAAPGG
                     EGGPARASGLALNLINANLFGDQTNSAGDAFGGAGGKAPAGDGYYAS"
     gene            1418..1687
                     /locus_tag="VHH53_14590"
     CDS             1418..1687
                     /locus_tag="VHH53_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291381.1"
                     /translation="MSEAPSCTELDGQHVELLRTCTVLSLFSAASGGRGGNGGAVAPG
                     GVGKGRARPQPHQHQLGRGTDQHAGNGWCCQGGGSIGGAGGPAVS"
     gene            2015..>2275
                     /locus_tag="VHH53_14595"
     CDS             2015..>2275
                     /locus_tag="VHH53_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291382.1"
                     /translation="MAKGTELIGEYQGSGFQEPRFIVRRVDGQVIQLPRLLFLLASLL
                     NGQRDLQALAALLTAEFGKVVAPQQVSYLIDNRLRPVGIVAAD"
BASE COUNT          382 a          678 c          771 g          444 t
ORIGIN      
        1 gatgcacccc cccggttttg ttcgccccgg cgcgggctcc ggtgctattg agcatcacca
       61 cgtccgggct ggtatgccta actcacggcc cgcactcccg gcacccgaca tgccacaaca
      121 ccagctccac cgtgcccacc acggtgcgcg ggcttccgtc tccgggtgcg tctgcctacg
      181 catgaagtgc tcaaccattc gcacctcgga acgcaaacgg cgccgccaag cccccgctgg
      241 agcccaccgc ggcccgcaca ccgaatcgat taatcgctcc tgggcatcct cggcaccgag
      301 gccagtgcct ggcccggcag cttcttgccc gtctgcaccg caatgactcg aactttcggc
      361 cgacacgagc gggtgtcgag cactccctgc tcgaccgtgg gcgtcaggac agtagcggag
      421 gtggaggtag gcgactcctc acacgagttg ggatgatcac ctgcagccct acgagatcag
      481 gaccgaagtt cagcggagcg acacgcaaca gatcgcatcg acgccgcaaa cccaggtccg
      541 cgggtcattc taggtactcc gtatctggac tctcggtaga tgtcgccgcc cactacgtat
      601 tcggcaagta ggccacttct aggtagcgcg ccggaggagt tacgtgtgac tactgattgc
      661 cacactgggt aaccgatggg caaagtatgt ggcgtaccga atcggcactg ccaccgatga
      721 agtgtttgca gtcccggggt cacacgaccc acctttccta ggaggattta aatgtctgac
      781 gcactgaccc ttgctgagat cgacgcgcag cacgtggagc tgctgccggc acgtaccgtc
      841 ctgtcgctgt tcatgaccgc cggcggtggc ggtgctcggg gcggcaacgc tgccccgggc
      901 ggcgagggcg gtcccgccag ggcgagcggc ctcgccctca acctcatcaa cgccaacctg
      961 ttcggcgacc agaccaacag cgctggcgac gctttcggtg gcgccggcgg caaagccccc
     1021 gctggtgatg ggtactacgc cagttgagtc gaagctggtg tcgcagggcg atcacatgat
     1081 cgcctacggt gcggagcgcg cgggaggggt ggccggaccg gtcacccctc cctcgcgttt
     1141 gcctgggtgt tcaccagcgt gggtgcggat ccgaggggca ccgcagcacg ctggcgcgga
     1201 tctcaccata gatactcggt gttggaactc tccgtaactc ttctagcgga gtacatgttc
     1261 gacgcgtgcc gtgggactag gtagtcaccc agaagagtta agtgcctact actgattgcc
     1321 tacagggcgt cactgtgggc atggtggtgc agccggatcg gactgccacc gatgcggcgt
     1381 tgtatccacc gggggacgcc tttcctagga ggaactcatg tctgaggcac cgagctgtac
     1441 cgagctcgat gggcagcacg tggagttgct gcggacctgt accgtcctgt cgctgttcag
     1501 tgccgccagc ggcggccgtg gcggcaacgg tggcgcggtc gctcccggcg gtgtcggcaa
     1561 gggtcgggct cggcctcaac ctcatcaaca tcaacttggt cggggaacag atcaacacgc
     1621 cggtaacggc tggtgctgcc aaggcggcgg cagtatcggc ggtgccggcg gacctgccgt
     1681 gagctgactc gcgctgatgt cgctgggtga tcatgcggtt cgtgcggtga cgggaggggt
     1741 gactgggggc cacccctccc acgtggtgat gtggggcatg acctacgcaa tagggctaga
     1801 gaaagtaagt agtgaagagc cgtgccgcgt ggcgttgggc gtcggtaaaa tgtagtggcg
     1861 cggcgttcag caatgccact cacgatgtct ttcggtgcgg cagttttcga ttcggcaaag
     1921 tgggtttcga gtcggggagg gcgtcatcga tactgagcgt gtggtgctgg ggtctggggg
     1981 ccagcccgat gctgccgctg gtggagggcc gcggttggcg aagggaactg agctgatcgg
     2041 ggagtatcag gggtctggtt ttcaggagcc gagatttatt gtccgtcggg ttgatgggca
     2101 ggttattcag ctgcctcggc tgttgttttt gttggcgtcg ctgttgaatg gccagcgtga
     2161 tctgcaggcg ctggcggcgt tgttgactgc cgagttcggc aaggtggtgg ccccccagca
     2221 ggtgtcgtat ttgatcgata accgcttgcg tccggtgggc atcgtggcgg ctgac
//
LOCUS       DATADN010001138         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_450307, whole genome shotgun sequence.
ACCESSION   DATADN010001138 DATADN010000000
VERSION     DATADN010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450307"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VHH53_14600"
     CDS             <1..650
                     /locus_tag="VHH53_14600"
                     /inference="COORDINATES: protein motif:HMM:NF019034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HEX2291383.1"
                     /translation="EDLHVRMMRGAVARGHRTVVFKPHPTAPARYSRALETEAEKLGV
                     DLTVLDTPVLAEVLFERTRPALVIGCFSTALFTASVFYELPVARIGTELLLERLTPYQ
                     NSNRIPTTLADALLPDLDGGKDGDLLSAELLSDLVTAVGFTMQPQIYPDLRPQAEQFL
                     ARHLGPRTRRYFKKRRLTSLGLPGGIPQRLAFLPRNSTARSVVRRARALRKAVGR"
     gene            831..1985
                     /locus_tag="VHH53_14605"
     CDS             831..1985
                     /locus_tag="VHH53_14605"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2291384.1"
                     /translation="MTETVVSTPKPRPEVTVLVEPPGATTVPPRRKDSGGRLRALDGL
                     RLVAALMVAAYHYGGRDGEVSEAWGSSPKEQFPTLHEYFAYGCLGVQVFFVISGFVIC
                     MSGWGRPLKSFFASRASRLLPAYWVAVVLVTAVFALPMVAYKAVSPSDALVNLTMLQM
                     PLGVDRVLGVCWTLWAEVRFYALFALCVVLPGANRRRVIMFCAGWMLATVIAQNANMP
                     LLDIVVMPEYAPFFIGGVGLYLVHLDRRDAYGWGIVAVSWLIGQHFAVQGLWHAPDAG
                     GFSYRSSIGIVLVVTFGFVAVAAIALGWLRWANWRWLTVAGALTYPFYLVHEHLGWVV
                     IHALHRGLGLPSAATFSLTVASMLLLAWLLNQYVEKPLTPRLRALLAKAR"
     gene            2049..>2499
                     /locus_tag="VHH53_14610"
     CDS             2049..>2499
                     /locus_tag="VHH53_14610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2291385.1"
                     /translation="MTNAQATDTYPGELTDVPGWFPPLDQMLFTWFLERQQKQGMRGD
                     LLELGVYMGKSAILLGHHLQDGEKFTVCDLFEGDAPDGANQAEATKSYASLTQQAFER
                     NYLSFHDTLPTVIAAPSSVVSEKVAPDTCRFVHVDASHLYEHVYGDIG"
BASE COUNT          349 a          878 c          843 g          429 t
ORIGIN      
        1 aagaggacct gcacgtgcgg atgatgcggg gcgcggtcgc ccgcggtcac cgcacggtcg
       61 tcttcaagcc gcacccgacc gcgccggccc gctacagccg cgccctggag accgaggccg
      121 agaagctcgg tgtggacctc accgtgctgg acacgcccgt gctcgccgag gtgctgttcg
      181 agcggacccg gcccgcgctc gtcatcggtt gcttctccac ggcgctcttc acggcctcgg
      241 tgttctacga gctgccggtc gcacgcatcg gcacggagct gctgttggag cggctgacgc
      301 cgtatcagaa cagcaaccgg atccccacca ccctggcgga cgcgctgctg cccgacctgg
      361 acggcggcaa ggacggcgac ctgctgtcgg cggagctgct gtcggacctg gtgaccgcgg
      421 tcgggttcac catgcagccg cagatctatc cggacctgcg cccgcaggcg gaacagttcc
      481 tcgcccgcca tctcggtccg cgcacccggc gctacttcaa gaagcgccgt ctcacctcgc
      541 tcggactgcc cggcggtatt ccgcagcggc tggcgttcct gccccgcaat tccacggcac
      601 gcagtgtggt ccggcgggcg cgggcgctgc gcaaggcggt gggccgctga ggcggtcttg
      661 tcctgcggcc gggctcggcg acatccagat gaacccatgg ggaccggcag gcgaaggctg
      721 actccgtcga acgttttagc gtcaacctcc gccctagggt gacatcggcg ccctgcccgc
      781 ccctggcggc tcaggtgaag gacatacgag ctactacaga ggttttcagc gtgaccgaga
      841 cggtcgtcag cacccccaag ccccggcccg aagtcaccgt gctggtcgaa ccaccgggcg
      901 ccacgaccgt cccaccgcgc cggaaggact ccggcggccg gctgcgcgcc ctggacggac
      961 tgcggctggt ggccgcgctg atggtggcgg cgtaccacta cggcgggcgc gacggtgagg
     1021 tctccgaggc ctggggcagc tcgcccaagg agcagttccc gaccctgcac gagtacttcg
     1081 cctacggctg cctcggcgtc caggttttct tcgtgatcag cgggttcgtg atctgcatga
     1141 gcggctgggg ccggcccctg aagtcgttct tcgcctcccg cgcctcccgg ctgctgcccg
     1201 cctactgggt ggcggtcgtg ctcgtgacgg cggtgttcgc gctgcccatg gtggcctaca
     1261 aggcggtctc gccgagcgac gcgctggtga acctgaccat gcttcagatg ccgctgggcg
     1321 tggaccgggt gctcggggtg tgctggacgc tgtgggccga ggtccgcttc tacgcgctgt
     1381 tcgcgctgtg cgtcgtgctc cccggggcga accgccgccg ggtgatcatg ttctgcgccg
     1441 gctggatgct ggcgacggtg atcgcgcaga acgcgaacat gccgctgctc gacatcgtcg
     1501 tgatgccgga gtacgccccc ttcttcatcg gcggtgtcgg cctctacctc gtccacctcg
     1561 accggcggga cgcctacggg tggggcatcg tcgccgtgag ctggctgatc ggtcagcact
     1621 tcgcggtgca gggactgtgg cacgcccccg acgctggcgg cttctcctac cgttcctcga
     1681 tcggcatcgt cctcgtcgtg accttcggct tcgtcgcggt cgcggcgatc gcgctgggct
     1741 ggctgcgctg ggcgaactgg cgctggctga cggtggcggg ggcgctgacg tatccgttct
     1801 acctcgtcca cgagcacctc ggctgggtgg tgatccacgc cctgcaccgg ggcctgggtc
     1861 tgccgtccgc ggcgacattc tcgctgacgg tggcctcaat gctgctgctg gcgtggttgc
     1921 tgaaccagta tgtggagaag ccgctgaccc cacgactgag ggccctgctg gccaaggccc
     1981 gctgagctgt tcacgggacg ttcaaccaac cgtcggtttc agcaccgctc agggagaagg
     2041 ctcgtctcat gaccaacgca caagcgactg acacgtaccc cggcgaactc accgacgtcc
     2101 ctggctggtt cccgccgctg gaccagatgc tgttcacctg gttcctggag cggcagcaga
     2161 agcaggggat gcgcggagac ctgctggagc tcggcgtcta catgggcaag agcgcgatcc
     2221 tcctcggcca ccacctccag gacggcgaga agttcaccgt ctgcgacctg ttcgagggtg
     2281 acgcccccga cggggccaac caggcggagg cgacgaagtc gtacgcctcc ctcacccagc
     2341 aggccttcga gcggaactac ctctccttcc acgacaccct cccgacagtg atcgcggccc
     2401 ccagctcggt ggtgtccgag aaggtggccc cggacacctg ccgcttcgtc cacgtcgacg
     2461 cctcgcacct gtacgagcac gtgtacggcg acatcggcg
//
LOCUS       DATADN010001139         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1334846, whole genome shotgun sequence.
ACCESSION   DATADN010001139 DATADN010000000
VERSION     DATADN010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334846"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..478
                     /locus_tag="VHH53_14615"
     CDS             119..478
                     /locus_tag="VHH53_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEX2291386.1"
                     /translation="MTASAESDRGVATVWAAGAVAVLMSLAVFGLHLGTAMVARHHVE
                     SAADLAALAGAGMAVAGERYACTQARRVTDRMRVQLLSCRTQGWDVLVEVATRPAGWL
                     GDLGAATGRARAGPVTS"
     gene            complement(468..1343)
                     /locus_tag="VHH53_14620"
     CDS             complement(468..1343)
                     /locus_tag="VHH53_14620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2291387.1"
                     /translation="MAANLAVLYVVFGSTYLAIRVMVETIPALVGAGLRFLVAGLLLY
                     GWCTLRRGYIPRLDARQFGGTVLIGLLVIGGGLGLLTVGEKTVPSGLAALLIASVPAW
                     VVLLRAVNRERVRLVTGVGVVVGFAGVALLGGTSGEVALTGIVILLVAAVSEAIGSYY
                     TPRFSLPEDPILSSAVQMLVVGPVLLLAGLGVGEQIHPSLWSGHSLLALIYLIGPGSI
                     LAYASFVWLVSRAPASIATTYAYVNPVVALFLGWLILDEKITTGILVGAILIIVGVAA
                     VLSGENRSEERALTR"
     gene            complement(1388..>1702)
                     /locus_tag="VHH53_14625"
     CDS             complement(1388..>1702)
                     /locus_tag="VHH53_14625"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEX2291388.1"
                     /translation="ALDDPDVREILLPQLRADFAVAETYDPETTGGGDLTVPCPISAL
                     VGNTDRTVDHSTVGEWSAHTTSSFRQRTLPGDHFLLDSARPYLLRALRTDLAAALQGQ
                     PL"
BASE COUNT          298 a          529 c          634 g          241 t
ORIGIN      
        1 tgacggtccg gggcgacgag gtcaccgcgc gggtttcggc ggatccagta gcagggctcc
       61 tgccgggcat cgaggttggg gccgaggcag cggcggtgct ggaaccaggt ggcctgccgt
      121 gacggcatcc gccgagagcg accggggagt ggccacggtg tgggcagcag gggccgtcgc
      181 ggtgctgatg agcctcgccg ttttcggact gcatctgggc acggccatgg tcgcgcgtca
      241 ccacgtcgag tcggcagctg atctggcggc gctggccggg gccgggatgg cggtggccgg
      301 tgagcggtac gcctgcacgc aggctcgccg ggtgactgac cgaatgcggg tgcagctgtt
      361 gtcatgccgg acacagggat gggatgtgct ggtggaagtg gcgacccggc cagccggttg
      421 gctaggcgat ctcggggccg ccactggccg ggcacgggct ggaccggtca cctcgtgagg
      481 gcacgttctt ccgagcggtt ctcgccgctc aggacagcgg cgacgcccac gatgatgagg
      541 atggccccga cgaggatccc agtggtgatc ttctcgtcaa ggatcagcca tccgaggaac
      601 agcgcgacga ccggattgac gtaggcgtag gtggtcgcaa tagatgccgg tgcccgcgag
      661 accagccaga cgaatgaggc gtaggccagg atcgagccgg gtccgatgag gtagatcaag
      721 gcgagcaggc tgtgacctga ccacagcgac gggtggatct gttcaccgac cccgaggcct
      781 gccagcaaca ggacggggcc gactaccagc atttgcaccg cagaggacag gatcgggtcc
      841 tcaggtagtg agaagcgtgg cgtgtagtac gacccgatgg cttcggagac ggcggcgacc
      901 agcaggatca cgatgcccgt cagcgcgacc tctccgctgg tgccgccgag cagcgccacc
      961 ccggcgaacc ccaccaccac cccgacgccg gtgactagcc gcacccgttc gcggttgacc
     1021 gcgcgcagca gcactaccca ggcgggtacc gaagcgatga gtagagcagc gagtccggac
     1081 ggtacggttt tctcaccaac ggtcagcaga cccagcccac cgccgatgac gagcagcccg
     1141 atcaggaccg tcccgccgaa ctggcgtgca tcgagccgag ggatgtaccc gcggcgcagc
     1201 gtgcaccagc cgtagagcag cagccctgcg acgaggaacc gcaggcccgc tccgacgagg
     1261 gcggggatcg tctcgaccat cactctgatg gcgaggtagg tcgagccgaa aaccacgtaa
     1321 agcaccgcca ggttggcggc cacggccggg cttcgtagag gattggctgt gcgagtgtcg
     1381 tgggcactca cagcggttgc ccctggagcg cggcggcgag gtcagtgcgc agtgcgcgca
     1441 gcagatacgg ccgggcgctg tcgagcagga agtggtcgcc gggcagcgtc cgctgacgaa
     1501 acgaggacgt ggtgtgcgcc gaccactcac cgaccgtgct gtggtcgacc gtgcggtcgg
     1561 tgttgcctac cagcgccgag atcgggcacg gcaccgtgag atcaccccca ccggtggtct
     1621 cggggtcgta ggtttcggcg accgcgaagt cggcacgcag ctgcgggaga aggatctcgc
     1681 ggacgtccgg atcgtccagg gc
//
LOCUS       DATADN010001140         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1204383, whole genome shotgun sequence.
ACCESSION   DATADN010001140 DATADN010000000
VERSION     DATADN010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204383"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VHH53_14630"
     CDS             <1..740
                     /locus_tag="VHH53_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX2291389.1"
                     /translation="MHVEAFRERFPDAEGPARGGVQMPAWHHHTPAGDGASLVNIGVG
                     PANAKTVTDHLAVLRPDIMMMIGHCGGLRNHQRLGDFVPATAYQRADHVLDEVLPADV
                     PVIPNHRLNTFLLDALEAADARYRLGVVYTTDNRNWEFNQRITLAAMRASRSAAVDME
                     PATIAANGFRYRIPNAALLCVSDKPLHNSPKLSASASAFYEISKRQHLGIALACLDRV
                     RREHPDGLPSHDIRSADEPLFGVDGTG"
     gene            complement(782..>1672)
                     /locus_tag="VHH53_14635"
     CDS             complement(782..>1672)
                     /locus_tag="VHH53_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2291390.1"
                     /translation="IARGLFVGVATLDVVQRVARRPGVDEKVVAQRSDIATGGPAAAA
                     AITFGALGGHSVLLSALGPGPVGRLVAEKLAEVGVQVVDAWAGGADLSISAITVLEST
                     GQRSVVSRNAQDLTAVVPAAVPALVRETDVVLIDGHHPDLALAAARAAQATGVPVVLD
                     CGSAKPVYAGLVPLADAAVCSANFTAEGRAGFDAVSVALLADGARLVAMTAGAAPVRW
                     RTREAAGTVEVPAVAVRDTLGAGDVLHGAVAFARARGVTDPEPSLSFGVAVASLRVQH
                     VGPLTWRDDRRLGILVAQLG"
BASE COUNT          273 a          615 c          553 g          231 t
ORIGIN      
        1 caatgcacgt tgaggctttc cgggagcggt tcccggacgc ggagggcccc gcacgcggcg
       61 gcgtgcagat gccggcctgg catcaccaca cccccgccgg tgacggggcc agcctggtga
      121 acatcggagt cgggccggcc aacgcgaaga ccgtgactga ccacctcgcg gtgctacggc
      181 cggacatcat gatgatgatc ggacactgcg gtggcttgcg gaatcaccag cgcctcggcg
      241 acttcgtgcc cgcaacggcg tatcagcgcg cggatcatgt gctcgatgag gtgttgcccg
      301 cggacgtgcc ggtgatcccg aatcaccggc tgaacacctt cctgctcgac gccctggaag
      361 ccgctgatgc gcggtaccgg ttgggggttg tgtacaccac cgacaaccgc aactgggagt
      421 tcaatcagcg catcacgctg gctgcgatgc gggcttcgcg cagcgccgcg gtcgacatgg
      481 agccggcgac gattgccgcc aacgggttcc ggtaccgcat ccccaacgcg gcgctgctgt
      541 gcgtctcgga caaacccctg cacaactcgc cgaagctctc cgccagtgcc tcggcgtttt
      601 acgagatcag caagcgccag cacctcggga tcgcgctcgc ctgcctggac cgggtgcgcc
      661 gcgagcaccc cgacggactg cccagtcacg acattcgctc tgccgacgag ccactgttcg
      721 gggtggacgg cactggctga ggcgggccat ctcgacctgt ttcgcgattc ctctggatcg
      781 gttatccgag ttgtgcgacc aaaatgccca gtcgtcgatc gtcgcgccaa gtcagtgggc
      841 cgacgtgttg cacgcgcagc gaggcgaccg cgacgccgaa gctcaggctg ggctcggggt
      901 cggtcacccc acgtgcccgg gcgaaggcga ccgccccgtg cagcacgtcc ccggcaccga
      961 gagtgtcacg gacagcgacc gccggcacct cgacggtgcc ggcggcctcc cgggtccgcc
     1021 agcgcaccgg cgcggcgccg gcagtcattg ccaccaggcg ggcgccgtcg gccagcagcg
     1081 ccacggacac cgcatcgaag cccgctcggc cctctgccgt gaagttcgcg gagcacaccg
     1141 cggcgtcggc gagcggcacg agcccggcgt acaccggctt ggcgctgccg cagtccagca
     1201 ccaccgggac cccggtggcc tgggcggccc gcgcagccgc caatgccaga tcgggatggt
     1261 ggccgtcgat cagcaccacg tcggtctccc ggaccagcgc aggcacggcg gcaggcacca
     1321 cagcggtgag gtcctgggca ttgcgggaca ccaccgagcg ctgtccggtg ctctccagca
     1381 cagtgatcgc cgagatgctg agatccgcac cgcctgccca ggcgtccacg acctgtacgc
     1441 ccacttccgc cagcttctcc gcaaccagcc gaccgaccgg gccaggaccc agcgcggaga
     1501 gcagcacgct gtgcccaccc agcgcgccga aggtgatcgc ggcagcagca gccggtcccc
     1561 cggtggcgat gtcgctgcgc tgggcgacga ccttctcatc gacaccgggc cgtcgagcca
     1621 cccgctgcac cacgtccagg gtggccactc caacaaacag cccgcgagct at
//
LOCUS       DATADN010001141         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_743551, whole genome shotgun sequence.
ACCESSION   DATADN010001141 DATADN010000000
VERSION     DATADN010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743551"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VHH53_14640"
     CDS             <1..723
                     /locus_tag="VHH53_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX2291391.1"
                     /translation="VVDAPAGATTHVDSGRVSGKTYQYRIRSCNVVGCSAYVFTPSVT
                     VGPIPTAPSGPIGNVPVANRINLRWNDLSTDENRFELSRRRRNTDGTWQGYAQVGLLP
                     ANTTTAVDSAVQTTYAYQYRLRACNAYGCSAWAEGPVVSLPATPGALTVDAPTSPTSL
                     TLHWGDRSTDETSFQVGRHTQNANGTWGPYTTIKVLGANAVTYTDNTVGPSQTHQYRV
                     RACNAAGCSPWRLGGYVTTPAS"
     gene            874..>1500
                     /locus_tag="VHH53_14645"
     CDS             874..>1500
                     /locus_tag="VHH53_14645"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX2291392.1"
                     /translation="MLPARRLTRARALLLADEGRTDDAIAEALHISRPTVERLRKRFV
                     DGGLEWALKDRPRKGAPRKLSGRDEALLVAMACSDAPEGRTCWTMQLLADRLVETNVV
                     ETISEETVRRILKNDLKPWQKEQWCIPSVGAEFVYHMEDVLDLYEEPYDPRFPVVTFD
                     ELPYQLTDEVRLPLPARPGQPERYDYEYRRRGTCNLYHHFEPLRGWRHV"
BASE COUNT          273 a          490 c          511 g          226 t
ORIGIN      
        1 gtcgtcgacg cgccggccgg cgccaccacg cacgtggact cggggcgggt gagcggcaag
       61 acgtaccagt accgcatccg ctcgtgcaac gtggtggggt gctccgcgta cgtgttcacc
      121 cccagcgtca cggtggggcc gattcccacc gcgccctcgg gtcccatcgg caacgtgccg
      181 gtggccaacc gcatcaacct gcgctggaac gacctgagca cggacgagaa ccgcttcgag
      241 ctgtcgcggc ggcggcgcaa cacggacggc acctggcagg gctacgcgca ggtcgggctg
      301 ctccccgcca acaccaccac ggcggtggac tcggcggtgc agacgacgta cgcgtaccag
      361 taccggctgc gcgcctgcaa cgcgtacggc tgctcggcgt gggccgaggg gccggtggtg
      421 agcctgccgg ccacgccggg cgcgctgacg gtggatgcgc ccacgtcgcc gacctcgctg
      481 acgctgcact ggggcgaccg cagcacggac gagacctcgt tccaggtggg ccgccacacg
      541 cagaacgcga acggaacgtg gggtccgtac acgaccatca aggtgctggg cgcgaacgct
      601 gtcacctaca ccgacaatac ggtgggcccg tcgcagacgc accagtaccg cgtgcgtgcc
      661 tgcaacgccg ccggctgctc gccctggcgc ctgggcggct acgtgacgac gccggccagc
      721 tagtagttag gcacaaagga cttgatgtaa agaaagtaaa catgcatggt atgggaaccc
      781 caaccccgga gggttccatg ccgaagaagc tttacgtggt tgacctgacg cccgaggagc
      841 gcgcagaact tctcggtggt ctgaaccgag gcagtgctcc cagccaggcg actcacccgc
      901 gcgcgggcac tccttcttgc ggatgaaggc cgcacggacg acgcgatcgc cgaagcattg
      961 cacatcagcc ggcccacggt tgagcgtctg cgcaagcgct ttgttgatgg tgggctggaa
     1021 tgggcgctga aggaccggcc tcgcaagggc gcgccgcgca aactctccgg ccgggacgag
     1081 gcgctgctgg tggcgatggc gtgcagcgat gcaccggagg gtcggacgtg ctggacgatg
     1141 caactgctgg cggaccggct ggtggagacc aacgtcgtcg agaccatctc cgaggagact
     1201 gtgcgtcgga ttttaaaaaa cgacttgaag ccctggcaga aggagcagtg gtgcatccca
     1261 agcgtgggag ccgagttcgt ctaccacatg gaagacgtgc tggatctcta tgaagagccg
     1321 tacgatccac gttttcctgt ggttaccttc gacgaactcc cgtaccagct caccgacgag
     1381 gtgcggttgc cgttgccggc acgccccggg cagccagagc gctacgatta cgagtaccgc
     1441 cgtcgcggta cctgcaacct ctaccaccac ttcgagccgc tgcgtggctg gcggcacgtg
//
LOCUS       DATADN010001142         2870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1335510, whole genome shotgun sequence.
ACCESSION   DATADN010001142 DATADN010000000
VERSION     DATADN010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335510"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VHH53_14650"
     CDS             complement(<1..1106)
                     /locus_tag="VHH53_14650"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX2291393.1"
                     /translation="MGDFADLRIGVEEEFHLVDLQTRKAAPAVDTMLAQLGGESFAPE
                     LQRSLVEANTLPCESLDELRAELCRLRRRLSEVAEPLGLGVVATGTVPLVDLLGTDIS
                     AGTRYERMQHEYQLLVREQHICGAQVHVDVPDRDVAVAVVQHVALHLPTLLALSASSP
                     YWRAVDSGYASYRCMVWQRWPTAGPPGPMRTAADYDAMVADLIASGTISDPGMVYFDI
                     RPSAHLPTVELRICDACPVVDDVVLITGLFRALVQRARGWVEEGRAFPQTRHELLRAA
                     TWRAARSGLEGDLVDLADQSGPALVPPSRLVEGLVGDLRPELEASGDWKQVLELSQRA
                     LAAGSSAARQRRMFGRAANSLTWLTIWSPRLAASR"
     gene            complement(1397..1681)
                     /locus_tag="VHH53_14655"
     CDS             complement(1397..1681)
                     /locus_tag="VHH53_14655"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291394.1"
                     /translation="MDGQPTHCPYCSLQCGIAMRVAPDRLHLAPQEYFPTNRGRLCSK
                     GWTAAELLDHPQRLTAPLVRDRRDAPLEPSTWDDALNQVAQVKLPKVPVR"
     gene            2325..>2870
                     /locus_tag="VHH53_14660"
     CDS             2325..>2870
                     /locus_tag="VHH53_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX2291395.1"
                     /translation="MTAETATGRSKRLTPGAQRILDVASELFYWRGIHAVGVDTIAAE
                     SGVTKRTLYDQFGSKDGLIVAYLEARNRRWRALITSRLDAPHADPVQRALVPFDVLFE
                     WLAPSSRGCSFVNAFAELSEPEHPGHQAVIAKKKWLRALFRELLAEAGAKDPDELAVQ
                     LLSLHEGAVVCYSMAGEANAAA"
BASE COUNT          491 a          976 c          925 g          478 t
ORIGIN      
        1 cgactcgccg cgagtctggg cgaccagatc gtcaaccacg tcagtgagtt cgccgcgcgg
       61 ccgaacatcc ggcgttgacg ggccgcggag ctgcccgcgg caagggctcg ttgggacagc
      121 tcaagcacct gtttccagtc gccggatgcc tccagctccg gtcgcaagtc gccaaccagt
      181 ccttcaacga gccgcgacgg cggcaccagg gccggcccgg actgatcggc aaggtcgacg
      241 agatcgccct ccagcccgga ccgagccgcc cgccaggtgg ccgcccgcag cagctcgtgg
      301 cgggtctggg gaaacgccct gccctcctcg acccaaccgc gggcccgctg caccagcgcc
      361 cggaacaggc cagtgatgag caccacgtcg tccaccacgg ggcaggcatc gcagattcgc
      421 agttcgacgg tgggcaggtg cgccgacggg cggatgtcga aatagaccat gccggggtca
      481 ctgatcgttc ccgaggcgat caggtcggcc accatggcgt cgtagtcggc ggcggtacgc
      541 atcggccctg gcgggccggc agtcggccaa cgctgccaga ccatgcagcg atagctggcg
      601 taaccgctgt cgaccgcccg ccaatacggc gagctggccg acagcgcgag aagggtcggt
      661 aggtgcagcg ccacgtgttg cacgacggcc accgcgacgt cccgatcggg cacgtcgacg
      721 tgtacctgag ctccgcagat gtgttgctcg cggaccagca gctggtattc gtgctgcatc
      781 cgctcgtagc gcgtgccggc cgagatgtca gtgcccagta ggtcgactag tgggacagtt
      841 ccggttgcca ccacgcccag cccgagtggc tcggcgacct cgctcaaccg gcggcgcagc
      901 cggcacagtt cggcgcgcag ctcatcgagg ctctcgcaag gcaacgtgtt ggcctcgacc
      961 agggagcgct gaagctccgg tgcgaacgac tcaccgccca actgcgcgag catggtgtcg
     1021 accgccggcg ctgctttcct ggtctgcagg tcgacgaggt ggaactcctc ctcgacgccg
     1081 atgcgcaagt ccgcgaagtc gcccatgggc tccgacgcta ggggattgtt gttacaggcg
     1141 ctttgcctcg aggtcgcagc acgagctcgc cgtcctgcac agccagcagc ggacacacca
     1201 cctgctgccc gtcgatgaga ccgtcagcga gcggaccacc gcggtccggg gagactgccg
     1261 ccaacgcccg tgcccatcct tcgcgcagcc ggaacacgcc acctgctcgt caccgacggc
     1321 gaatgcgcca ccctcgccga ggggatctga tccaccgggc caagtctgta cagcccggtg
     1381 ccattcgctg acccgctcac cggaccggta ccttcggtaa cttgacctga gcaacctggt
     1441 tcagtgcatc gtcccaggtg ctcggctcca gcggcgcgtc gcgccggtca cggaccaggg
     1501 gcgcggtcag ccgctgcggg tggtcgagca actcggcggc agtccacccc ttgctgcaca
     1561 gcctgccgcg gttggtcggg aaatattcct gaggagccag gtggagccgg tctggggcga
     1621 cccgcatggc aatgccgcac tgcagcgagc agtacgggca gtgcgtcggc tggccatcca
     1681 cacacctcta ccgtgcgcct accgcgtttc catgctgttg tgtaccggtt acggctaagc
     1741 cttagacggc tcgtctcacc ggttgcggga gctaccacgt ggtgccatca ggagctcgac
     1801 agacgtcgtc atgaccttca ggcagatgac acagccactc cccgtcttca gcgaggctgt
     1861 tgatgtgcgc cagtgagcta ccgcagcggc ggaagtcgac tgagcttggg tcttgggcga
     1921 gcgtggccag cgagtgagtc tccatggcga tggacgttaa tgtcgctaaa accgtcatag
     1981 cctgcgctgc gccaattggt gcggttatgg cggtgaacgg ttgagaagcc gatcggcttt
     2041 acgtctgaaa agcggacatc tcatccttgt acgcctagcg acggcctgct gcccaggcat
     2101 gaaccttgac gctggcggtt ccaggccgtt ggttcggccg ttgccgggca ccttcgggcg
     2161 ccggcaatcc gtgccggatc gcgttgccgg cgtggatccc gtagccgacc tctcgcacca
     2221 gctcgcccag tctccccatg atccgccgca tgtccactcc tatactgaac tgtgaagtaa
     2281 cttctactgt aggtatacag gtctgtgaat gcaagggctc gatgatgact gcggagaccg
     2341 ccaccggacg gagcaagcgc ctgactccgg gcgcccaacg catccttgac gtcgcctccg
     2401 agctgttcta ctggcgaggc atccacgcgg tcggagtgga caccatcgcc gccgaatccg
     2461 gagtcaccaa acggaccctc tacgaccagt tcggttcgaa ggacggcctg atcgtggcct
     2521 acctggaggc ccgtaaccgc cgctggcgcg cactcatcac gtcacgtctg gatgccccgc
     2581 atgccgaccc ggtgcaacgc gccttggtgc catttgacgt gctgttcgag tggctcgcgc
     2641 ctagcagcag gggctgcagc ttcgtcaacg ccttcgccga gctgtccgag ccagaacacc
     2701 ccggccacca ggcggtcatc gcgaagaaga agtggctccg agctctgttc cgtgagctgc
     2761 tcgctgaagc aggggccaag gacccagacg aactcgctgt gcagttactg agcctgcacg
     2821 aaggagccgt cgtctgctac tccatggcgg gcgaggcgaa cgccgcggcc
//
LOCUS       DATADN010001143         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1204836, whole genome shotgun sequence.
ACCESSION   DATADN010001143 DATADN010000000
VERSION     DATADN010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204836"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1029)
                     /locus_tag="VHH53_14665"
                     /pseudo
     CDS             complement(1..1029)
                     /locus_tag="VHH53_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558251.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          268 a          556 c          484 g          274 t
ORIGIN      
        1 gccgaccgat ccgacggagg agtcgatgcc ggcttcggcg agccggtcgg taacgcgatc
       61 ggggtgtatt gggcgagccg gcgtcattgt ggtggatcaa cccggcgagg tctttaaccc
      121 cgtcacggtg tcgggtccac atggcctgat ccaacgcatc caggacaaga gctgtggtca
      181 tcgtggtggc cgcgcgccac ccgaggatgc gccgggcgta ggcgtcgatc acaaaagcca
      241 catagaccat gcccgaccag gtcggcacat acgtaaagtc cgcgacccac agctggttgg
      301 gtgtgcgcgg tgcgaagtgg cgattgacca gatccgtggg ccgagccgcg gccgggtcag
      361 cgcaggtggt tttgcaccgc ttgccgcggc gcaccccgcg cagccccagc tttgtcatca
      421 accgctgccc gctacagcgg gccacgacga tgccctcccg gtttagcgcg agccagactt
      481 tccgagcacc gtagacgccg tagttgctgg cgtgcacccg cacgatttca accttcagat
      541 cctcatcgcg tagcgcttga cgtgatggca accgcccttg ccgcgtcgta gtaggtcaac
      601 gggcgatcgg cgcaccgtgc tcagtgagca cggcacagat cgactcgaca ccccaacgca
      661 gcccgttggg cccgacgcgg tgacccttgt gctcgtcgat gaacctcacc agcgccgctg
      721 tggccggtcg agttcggccg cgaagaaagc cgaggcggcc ttgaggatct cattcgctcg
      781 ccgcagcttg gccacctcac gcttgaggcg cttgatctcc gcgtgctcct cgctggtcac
      841 cccggcccgc tgaccagagt cgacttcagc ccgacgcacc cacttacgca gcgtctccgc
      901 ggtcccaata cccagcttct ccgcgacggc ctcgatcgcg ccccactgcc agccgtagtt
      961 agaccgcacc tcagcgacca tccgcaccgc gcgctcacgc agctccggtg gatagttcga
     1021 tcttcccatg gccaagaccc catcgccttc tcaacagatg gagcctccgg acacgccggg
     1081 gggattcatc cgcgtgccgg ggagtgtcat tgcatgccgc tcaactgcgg cgacgtgcca
     1141 atgtgtatcg gctggtgcgt cgtggtttcg tcgctttcgc tgacggctcg ctgacgacga
     1201 tcatgctaag caccagtctg gaaggctgca ctcggtggca gctcggcgcg gggcatcctg
     1261 ctctcgctga tcgtcgaccc ctgggttaga agtcggcacg cagccgtctc acacagtcca
     1321 cccgcaccgc cgacatccca cctgaccagc cgccagcgac ggcccaccgt ctcagcgctt
     1381 ccgcacagta ccgccgagtg gcatcgcggc ttggtcacga atctggtcac caacgcccat
     1441 tcacatgaga cgttcccgcc cgggcatgag gcggcgaagg aagtcggcgt accgcgttgg
     1501 tcacactctg cggaagtccg gtcccacgga cgcttacttt taaggcccgc gacgtgccgg
     1561 ccagccggga ccgccggagc ag
//
LOCUS       DATADN010001144         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1335852, whole genome shotgun sequence.
ACCESSION   DATADN010001144 DATADN010000000
VERSION     DATADN010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335852"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VHH53_14670"
     CDS             complement(<1..1075)
                     /locus_tag="VHH53_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEX2291396.1"
                     /translation="MRVGDSLLKLLGLCLLAGVLVAGMLFPIVGAVGVVSNRASDAID
                     SVSADLVATDPALMTTVADAEGRPIAYLYDQYRIPVTADQISPTMKAALIAIEDKRFY
                     EHNGVDWRGTMRAAVNNQTTGDVQGASTLTQQYVKNYLINVVYRGDEPEKKVEQKRAR
                     EQTITRKLKEARIAIQLEQKMTKDDILTGYLNIVEFAYQVYGIGAAATSYFHTTPDKL
                     TVAQAALLAGMVNNPSRYNPWQYPEETLKRRNLVLDKMVENQKLSAQAGDAAKKEPLG
                     VVEGGPDKPAANCVGAGTEFGFFCQYVEDYLKKLGFPHEQLYAGGYTILTSFDANATR
                     LAKEAAEAQVPKDLEGIANTMAIV"
     gene            1307..1606
                     /locus_tag="VHH53_14675"
     CDS             1307..1606
                     /locus_tag="VHH53_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX2291397.1"
                     /translation="MQSDQADWRVNALCRDEDPDGLFVRGKEQRKAKLVCVACLVRTE
                     CLAEALDNRIEFGVWGGMTERERRALLRRRPDVTSWRELLEDARRQHTEEDIRVG"
     gene            complement(1603..>1909)
                     /locus_tag="VHH53_14680"
     CDS             complement(1603..>1909)
                     /locus_tag="VHH53_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX2291398.1"
                     /translation="DSMPLAGLVVNRTHPVLSALPGPSALAAADRLDGSGDAPLAAAV
                     LRLHADRVAVADREQRLVARFTKAHPVVSVVTVPAMPADVHDLHGLRTIGEKLAASP"
BASE COUNT          290 a          627 c          660 g          332 t
ORIGIN      
        1 tgacgatcgc catcgtgttg gcgatgccct ccaggtcctt gggcacctgg gcctcggccg
       61 cctccttggc cagccgggtg gcgttggcgt cgaagctggt caggatggtg tagccgccgg
      121 cgtagagctg ttcgtggggg aagcccagct tcttgaggta gtcctcgacg tactggcaga
      181 agaagccgaa ctcggtgccg gcgcccacgc agttggcggc gggcttgtcc ggcccgccct
      241 cgacgacacc cagcggctcc ttcttggcgg cgtcgccggc ctgggcggac aacttctggt
      301 tttccaccat cttgtcgagg accaggttgc gccgcttgag cgtctcctcc gggtactgcc
      361 acgggttgta ccggctgggg ttgttcacca tgccggccag cagcgctgcc tgcgccacgg
      421 tcagcttgtc cggtgtggtg tggaagtacg aggtggcggc cgcgccgatg ccgtacacct
      481 ggtaggcgaa ctcgacgatg ttgaggtagc cggtgaggat gtcgtccttg gtcatcttct
      541 gctcgagctg aatggcgatc cgcgcctcct tgagcttgcg ggtgatggtc tgctcccgcg
      601 cccgcttctg ctcgaccttc ttttccggct cgtccccccg gtacaccacg ttgatcaggt
      661 agttcttcac gtactgctgg gtgagcgtgg acgcgccctg gacgtcaccg gtggtctggt
      721 tgttcaccgc ggcccgcatg gtgccccgcc agtccacccc gttgtgctcg tagaaccgct
      781 tgtcctcgat ggcgatcagc gcggccttca tggtcggcga gatctggtcg gccgtcaccg
      841 ggatgcggta ctggtcgtac aggtacgcga tcggtctgcc ctcggcatcg gcgaccgtgg
      901 tcatcagggc cgggtcggtc gccaccaggt cggccgacac gctgtcgatc gcgtcgctgg
      961 cccggttgga caccacaccc accgccccga cgatcgggaa cagcatgccg gccaccagaa
     1021 cgccggcaag aagacacaat ccgagaagct tcagcaggct gtctcccact cgcacgggcc
     1081 caagcgtacg cgggaccagg ccagagcacg ggatgcgccg aagaactgtt gacattaggt
     1141 aacggctagg cgggcccggt gttcggccgg gaaccagcgc cctctagagt ccgtcaacgt
     1201 ctcacgatgg cgctgaacgg tcggaccgtg cccgcggccg accctaggca accggtgccg
     1261 gggggcaccg gcaacgaggg ccgagacgaa gctaggggtg ggggtcatgc aaagtgacca
     1321 ggcagattgg cgggtcaacg cgctgtgccg cgatgaagac cctgatggat tgttcgtccg
     1381 cggtaaggag cagcgcaagg ccaagctggt gtgcgtcgcg tgtctggtac gcaccgaatg
     1441 ccttgccgag gccttggaca accgcatcga attcggtgtc tggggcggaa tgaccgagcg
     1501 tgagcgtcga gctctgctgc gtcgccggcc ggacgtgacc tcctggcggg aactgcttga
     1561 ggacgcccgc cgccagcaca ccgaagagga catccgggtc ggttagggag aagcggccag
     1621 cttctctccg atcgttcgca gcccgtgcag gtcgtgcacg tcagccggca tcgcgggcac
     1681 cgtgaccacc gagaccacgg gatgcgcctt ggtgaacctc gcgaccaggc gctgttcccg
     1741 gtcggcgacc gcgacccggt cggcatgcag ccgcagcacc gcggccgcca gcggcgcgtc
     1801 gccggacccg tccagccggt ccgccgcggc cagcgcgctc ggaccgggca gggcggacag
     1861 caccggatgg gtccggttga ccaccagccc ggccagcggc atcgagtcc
//
LOCUS       DATADN010001145         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_126225, whole genome shotgun sequence.
ACCESSION   DATADN010001145 DATADN010000000
VERSION     DATADN010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126225"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="VHH53_14685"
     CDS             complement(<1..150)
                     /locus_tag="VHH53_14685"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX2291399.1"
                     /translation="MMRWSPGRYGRAFASGMAATDALLRATCRPGDHLIIPDDAYGGT
                     FRLINK"
     gene            101..1006
                     /locus_tag="VHH53_14690"
     CDS             101..1006
                     /locus_tag="VHH53_14690"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX2291400.1"
                     /translation="MPDANARPYRPGDHLIIPDDAYGGTFRLINKVFRHWGIEHTAVA
                     LSHVDAVRAAVRPTTRLVWCETPTNPLLSIADIVALAGVAHEAKARLVVDNTFASPYL
                     QTPLALGADVVLHSTTKYLGGHSDVIGGALVTDDGALDEQVAFLQNGAGAVPGPFDAW
                     LILRGIKTLAVRMERHCDNAERVAAALQAHPEVIDVRYPGLDTHLGHEVAAKQMRRFG
                     GMVSFTVRGGAQAALAVCARTRVFTLAESLGGVESLIEHPGRMTHASTAGSQLEVPAE
                     LVRLSVGIEDVDDLIDDLLAALGQR"
     gene            complement(1060..1293)
                     /locus_tag="VHH53_14695"
     CDS             complement(1060..1293)
                     /locus_tag="VHH53_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291401.1"
                     /translation="MIRSGRWMPVVVTTGVAALLACAAIITANQAGCDEPGRYVSAPG
                     GVQLIGGCLNSADLPVAPPPVQQPAPPVQPLGD"
     gene            1405..>2269
                     /locus_tag="VHH53_14700"
     CDS             1405..>2269
                     /locus_tag="VHH53_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX2291402.1"
                     /translation="MELISVEQVEAARETLHGVVRRTPLKQFRVLEQRCGVPVHLKCE
                     NLQRTGSFKIRGAYVRIRALSTAARANGVVAASAGNHAQGVALAAAMLGTTATVYMPV
                     TVALPKLAATRGYGARVHLIGDIVEESIAAARKFADDTGAVFIHPFDHPDVLAGQGTV
                     GLEVLEQLPNVGTVLVPTGGGGLVGGIAAAVKGVKPDVRVIGVQAEGAAAWPASLAAG
                     APVRLRRRHTMADGIAVTELGPVSFAHVSALVDEVLTVGEEALSQALLLCLERAKMLV
                     EPAGAAAVAALL"
BASE COUNT          345 a          742 c          798 g          384 t
ORIGIN      
        1 cttgttgatc aggcggaagg tgccgccgta ggcgtcgtcg gggatgatga gatggtcccc
       61 gggccggcag gtcgcgcgca gcagggcgtc agttgcagcc attccggacg cgaatgctcg
      121 cccgtaccgg cccggggacc atctcatcat ccccgacgac gcctacggcg gcaccttccg
      181 cctgatcaac aaggtgttcc ggcactgggg gattgagcac accgcggtcg cgctgtcgca
      241 tgtggacgcg gtgcgcgccg cggtacgccc gaccacccgt ttggtgtggt gcgagacacc
      301 gaccaatccg ctgctgtcca tcgccgacat cgtggccttg gccggggtcg cgcatgaggc
      361 aaaagcgcgt ctggtagtgg acaacacctt tgcctcgccc tacctgcaga ctccgcttgc
      421 actcggtgca gatgtggtgc tgcactcgac cacgaagtac ctcggtgggc actccgatgt
      481 catcggtggc gcgctggtga ccgacgacgg tgcgcttgac gagcaggtgg cgttcctgca
      541 gaacggtgcg ggcgcggttc ccgggccctt cgatgcctgg ttgatcttgc ggggaatcaa
      601 aaccctggca gtgcgcatgg agcgacactg cgacaacgct gaacgggtcg ctgcagcgct
      661 acaggcgcat cccgaggtga tcgacgtccg ctatcccggc ttggacaccc atctcgggca
      721 tgaggtggct gccaagcaga tgcgccggtt cggcgggatg gtctcgttca ccgtccgtgg
      781 tggcgctcaa gcggcgctgg ccgtgtgcgc ccgcacccgg gtgttcaccc tggctgagtc
      841 gctcggcggg gtggagtcgc tgatcgagca cccaggacgg atgacgcatg cctccaccgc
      901 gggatcacag ctggaggttc ccgccgagtt ggtacggctg tcggtcggca tcgaagacgt
      961 cgacgacctc atcgacgatc tcctggccgc gctgggccag cgctgaacac gtctccctcg
     1021 cggcagtgtc taatcgccct gtgtctaatc accctgtgtc taatcaccca gtggctgtac
     1081 cggtggcgcg ggctgctgca caggcggcgg cgcgaccggc aggtcggcgc tgttgaggca
     1141 cccgccgatc agctgcacgc cgcccggggc ggacacatac ctgcccggct cgtcgcagcc
     1201 tgcttggttg gcggtgatga tcgcggcgca ggcgagcaac gcggccacgc cagtcgtgac
     1261 cacgaccggc atccagcggc cgctgcggat catgcccacc tcctgtgact tgcctgtgac
     1321 cttaccgcgc gcactgctgc acccatcggg ctgtgtcaca cgggcaggtg atcgaaggag
     1381 tccgcgctgg caggattgcc gaccatggag ctgatcagcg tcgagcaggt ggaagccgcc
     1441 cgcgagacac tgcacggagt tgtccgtcgc acgccactga agcagttccg ggtgcttgag
     1501 cagcgctgcg gggtcccggt ccacctcaaa tgtgagaact tgcagcgcac cggttcgttc
     1561 aagatccgcg gcgcttacgt ccggatccgg gcactgtcca cggctgcgcg agccaacggt
     1621 gtggtggcgg ccagcgccgg caaccacgcg cagggcgtag cgctggcggc ggcgatgctg
     1681 ggcaccacgg cgacggtgta catgccggtg accgtggccc tgcccaagct cgcggcgacc
     1741 cggggatacg gcgcgcgggt gcacctgatt ggcgacatcg tggaggagtc gatcgccgcg
     1801 gcgcgcaagt tcgccgacga caccggggcg gtgttcatcc acccgttcga ccaccctgac
     1861 gtactcgccg ggcagggcac agtggggctg gaagtgctcg aacagcttcc caacgtcggg
     1921 accgtgctgg ttcccaccgg tggcggcggg ctcgtcggcg ggatcgccgc cgctgtgaag
     1981 ggtgtcaaac ccgacgtgag agtgatcggt gtgcaagccg agggagccgc agcctggcct
     2041 gcctcgttgg ccgccggagc gccggtccgg ttgcgccggc ggcacaccat ggcggacggc
     2101 atcgctgtca cagagctggg accagtgagc tttgcacacg tgtctgccct ggtcgatgag
     2161 gtgctgacgg taggggagga ggcactgtcc caggccctgc tgctgtgcct ggagcgcgcc
     2221 aagatgctcg tcgaacccgc cggtgctgcc gccgtcgctg cactgctgg
//
LOCUS       DATADN010001146         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_126315, whole genome shotgun sequence.
ACCESSION   DATADN010001146 DATADN010000000
VERSION     DATADN010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126315"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..355)
                     /locus_tag="VHH53_14705"
                     /pseudo
     CDS             complement(24..355)
                     /locus_tag="VHH53_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
     gene            complement(543..1004)
                     /gene="grdA"
                     /locus_tag="VHH53_14710"
     CDS             complement(543..1004)
                     /gene="grdA"
                     /locus_tag="VHH53_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_085916664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(879..881),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HEX2291403.1"
                     /translation="MFDGKQVIAIGERDGISGTAIAACAKAAGAPVAYVATECFVUTA
                     AGAMDLSTQETIKRLVDQHGVDTLLVLLGAPDAESAGIAAETVVLGDPSWAGPLAGAQ
                     LGLPVYHILEDQVRDAVPAEVWEEQVGLMVDVLEVDAIADAVREFREQAPR"
     gene            complement(1005..1343)
                     /locus_tag="VHH53_14715"
     CDS             complement(1005..1343)
                     /locus_tag="VHH53_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2291404.1"
                     /translation="MTVVDATKENFDELVGSGLVLVDVWAESCRPCVALGPHMEKIAS
                     GNTDLTVVKVDASNARRLVMSLQVRGLPTILLLQDGQEVARISEPNLTTEQVDAWLAT
                     ALAELPAKEA"
     gene            complement(1364..>1997)
                     /locus_tag="VHH53_14720"
     CDS             complement(1364..>1997)
                     /locus_tag="VHH53_14720"
                     /inference="COORDINATES: protein motif:HMM:NF039601.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5940 domain-containing protein"
                     /protein_id="HEX2291405.1"
                     /translation="SLVVAGVVEQVAVVAGGSLPKLGMKFEGHLRHGLPVLEDVLGGT
                     AMLVTADDGVSPRIRLDAVGRHRVSSGGGGAQILHDLAVQPLEAIGARMLEVDDYATE
                     LHNPEITEPQGSGDVAARNYRTIAALAARRKEIARDGIESFVAERGMPGYAPTQGHIA
                     SAVCYLPHALSRLTAGPAERVLLLAKGSLFLGRMSQRSDGMSVLLERNGG"
BASE COUNT          333 a          711 c          615 g          338 t
ORIGIN      
        1 acgacgtggg ttctgtgctc acgtcagcct cgtggggcgt acatgatcac tgcgacaccg
       61 aacaagcagg tctggcaccg atgatgtcgt agcgatcggg ccgaaaccca tcgagggcga
      121 ttccccatgc cagcgagccc accatgaaga tgccgccata ggcagccagg atgcgtccga
      181 agtggggatc cggctgcagg gtggcgatga agccgtagag cccgagcgcg atgaccccgg
      241 cgccgatcca cgcgagcccg cggctctcac gcactccttg ccacaccagc cacgcgccgc
      301 cgatctcgaa cagggcggcg acgatgaaca ggccgacgga cttcgctagc agcatgagca
      361 cacctgctca ccgaacaggt ccgcaagcag cggttcggct ccgtcgggcg cacgtgctcc
      421 gagttcgacg tgatcgtcgg cgagctgtga gatgcggtag gtgcccacga tggcaacgct
      481 aaaccgtcga cccgagtcga aggtcacgtc gatcggggtg cctcggcgtc ccgtcgttgt
      541 ggttagcggg gcgcttgctc gcggaactcc cgtaccgcgt cggcgatggc atccacctcc
      601 agcacgtcga ccatcaatcc gacctgttcc tcccacacct cggcgggaac ggcgtcgcgc
      661 acctggtctt cgaggatgtg gtacacgggg aggcccaact gggccccagc caacggtcct
      721 gcccagctcg gatcaccgag caccaccgtc tcagcggcga tcccggcgct ctccgcgtcc
      781 ggtgcgccga gcagcaccag cagcgtatcc acgccgtgct ggtcgaccag gcgcttgatc
      841 gtctcctggg tggacaggtc catggctccg gcagccgttc aaacaaagca ctcggtggcg
      901 acgtaggcca ccggtgcacc cgccgccttg gcacaggccg caatcgccgt ccccgaaatt
      961 ccgtcgcgct ccccgatcgc gatgacctgc ttgccgtcga acattcacgc ctcctttgcg
     1021 ggtaactcgg ctagtgctgt tgcgagccac gcatccacct gttctgtggt gaggttgggc
     1081 tcggaaatcc gggccacctc ctggccgtcc tggagcagca ggatggtggg aagcccacgc
     1141 acctgcagtg acatgaccag ccgccgagcg ttgctggcat cgaccttgac cacggtcagg
     1201 tctgtgttcc cactcgcgat tttctccatg tgtggcccca gcgccacgca cgggcggcag
     1261 ctttcggccc acacgtccac gagcaccaac cctgacccga cgagctcatc gaagttctct
     1321 ttcgtcgcat ccaccacagt cacgtgtcag cctccgtcat gtgtcagcct ccgttgcgtt
     1381 ccagcagcac gctcatcccg tcggaccgtt ggctcatccg cccgaggaac aggctgcctt
     1441 tcgccagcag caacacccgt tcggccggtc ctgccgtcag ccggctcagc gcatggggca
     1501 ggtagcacac cgctgacgca atatgcccct gggtgggcgc gtaacctggc atcccacgtt
     1561 ctgcgacgaa agattcaatg ccgtcgcggg cgatttcctt gcgccgggcg gccagcgctg
     1621 cgatcgtgcg gtagttgcgc gctgcgacgt ctcccgagcc ctgcggctcg gtgatctccg
     1681 gattgtgcag ctcggtggcg tagtcgtcga cctccagcat gcgcgcaccg atcgcttcca
     1741 gcggctgtac cgccaggtcg tgcaggatct gcgcaccgcc gccgcccgag gacacccggt
     1801 ggcgcccgac ggcgtcgagc cggatccggg gcgagacgcc gtcgtcggcg gtcaccagca
     1861 tcgcggtgcc accgagcacg tcttccagca cgggcagacc gtgacgcagg tgcccctcga
     1921 acttcattcc cagcttgggt agtgagcctc cggcgacgac tgcgacctgt tcgaccacac
     1981 ccgccaccac caggctg
//
LOCUS       DATADN010001147         2265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1054679, whole genome shotgun sequence.
ACCESSION   DATADN010001147 DATADN010000000
VERSION     DATADN010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054679"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..1793)
                     /locus_tag="VHH53_14725"
     CDS             complement(234..1793)
                     /locus_tag="VHH53_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2291406.1"
                     /translation="MVDHIVVGGGHNGLVTGCYLARAGLDVVVLEQSAKLGGGSRTEE
                     LVPGYRFNTHSAAHNIINATGIVEELELRAAGLEYREMDPFSIAVHRDGAIVRFHRDV
                     ERTVESIREVDPVDADRYRAWMADTMPIVTVMCAGLEAGVGPPRQWHQLPARVWAATQ
                     ALARNGGPLGLAQLLLSPYGRVLEQHLTSDRVRAPISAFAAHASASPTTPGSAIYGLW
                     QAFYHQVGQWHAIGGAQGLIDALTRRLESYGGQWRTNAAVTRVTRDGQRVTGVGLESG
                     EHITTSCVITAINPKIALLALLDPPLSGAGGAQLRATHRSNAVQMLVLLATTALPAYT
                     HARTGDWNGLQSYVDSLGSLADGFAQAEARRLPDDPVPTYAFTPSELDDSLAPPNHHT
                     VYLACPTAPFKVRGGWEVAKEEFAERMIDTVVARAPGFRSTIVARAIRTPQDMADELR
                     WPGAHPMSLDISLDQLGWMRPTRALAGHDTPVAGLFITGAGTAPVGGIAGSPGRAAAK
                     AVLTWRRRNGR"
     gene            1894..>2265
                     /locus_tag="VHH53_14730"
     CDS             1894..>2265
                     /locus_tag="VHH53_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEX2291407.1"
                     /translation="MIEQILPRAVACAEAFGDPPDAVLFPQEEAVLRRAVDKRRREFR
                     TVRHCARQALCKLGLPPVAVLPGEHREPLWPPGVVGSMTHCAGYRAAALACSGDMLTV
                     GIDAEPHQPLPTDVLGLIALAE"
BASE COUNT          383 a          782 c          760 g          340 t
ORIGIN      
        1 cacccgcagg aggcgggacg acagtatttc tactgtacgg gggtagcgga agagctgccg
       61 ccgaaagatg ccgtgaacac ctccgtccgg gcaggtgcgg ccgacccggc catcgcgctg
      121 tcctcaccgg cgcctcggag aggaccatga aaccgacgct acgaagagtt cggttaccgg
      181 cccgcccaag ttcggttaac ggtcggcaaa atccgacatt accggaagca gcttcagcgc
      241 ccgttgcggc ggcgccaggt caggaccgcc ttggccgcgg cgcgaccggg cgaaccggcg
      301 atgccgccga ccggggcggt gccagccccg gtgatgaaca agccggctac gggcgtgtcg
      361 tggcccgcca gcgctcgggt gggacgcatc caacccagtt ggtcaaggct gatgtccagg
      421 gacatcgggt gcgcacccgg ccagcggagc tcgtccgcca tgtcctgggg ggtgcggatg
      481 gctcgcgcca cgatggtgga acgaaagccc ggcgcccgag ccaccaccgt gtcgatcatt
      541 cgctcggcga attcctcctt ggccacctcc cagccaccac gaaccttgaa cggcgccgtc
      601 gggcaggcga ggtagacagt gtggtggttc ggcggtgcga gggagtcgtc gagctcgcta
      661 ggcgtgaagg cgtaggtcgg tactgggtca tcaggcagcc gcctggcctc ggcttgcgcg
      721 aaaccgtccg ccaatgaccc caagctgtcc acgtaggact gcaacccatt ccagtcaccc
      781 gttctcgcgt gggtataggc gggcagggcc gtcgtggcca gcagtaccag catttgtacc
      841 gcgttgctgc gatgggtagc ccgcagctgc gccccgccgg cgccggacag tggcgggtcg
      901 agcagcgcga gcaacgcgat cttcggattg atcgcagtga tcacgcagga ggtagtgatg
      961 tgctcaccgg actcgaggcc taccccggtg acccgttgcc cgtcgcgggt cactcgggtt
     1021 accgccgcat tggtccgcca ctggccgccg taggactcca accggcgggt cagcgcgtcg
     1081 atgagtccct gcgcgccgcc gatggcatgc cactgcccga cctggtgata gaacgcctgc
     1141 cacaacccat agattgcgct gcccggtgtg gtgggagatg ccgaggcgtg cgcggcgaac
     1201 gccgagatgg gcgcgcggac ccggtcactg gtcaggtgct gctccaggac ccgcccgtag
     1261 ggcgacagca gcagttgcgc aagtcccagc gggccaccgt tgcgagccaa cgcctgcgta
     1321 gcagcccaca ccctggcagg caactggtgc cactggcgag gcggcccgac ccccgcctca
     1381 agcccggcac acatcacggt gacgatcggc atggtgtctg ccatccatgc ccggtaccgg
     1441 tcggcgtcaa ccgggtcgac ctcgcggatc gactcgacgg tccgctccac gtcgcggtgg
     1501 aaccgcacga tcgcgccgtc acggtgcacc gcgatggaga acgggtccat ctcccggtac
     1561 tccaacccgg ccgcacgcag ctccagctcc tcgacgatgc ccgtggcgtt gatgatgttg
     1621 tgcgcagccg agtgggtgtt gaaccgatag ccgggcacca gctcctcagt gcgggaacca
     1681 ccacccagct tggccgactg ctccaggacg accacgtcca gacccgcacg agccaggtag
     1741 cagccggtga ccaacccgtt gtgtccgcca ccgaccacga tgtgatcaac catccggtca
     1801 actgtacaaa tctgtccagt gcgcgcggcg cagcgacatc aggcaatgcg atgctcagcc
     1861 actgacaacg gtccgcacgg caaaggggga catgtgatcg aacagatcct tccccgtgct
     1921 gtggcctgcg ccgaggcgtt cggcgaccca cccgatgccg tgttgttccc gcaggaggag
     1981 gcggtgctgc gccgggcggt ggacaagcgg cgccgggaat ttcgcacggt ccgccactgc
     2041 gcccggcagg cactgtgcaa gctaggcctg ccgcccgtgg cggtgctgcc cggcgagcac
     2101 cgcgaaccgc tctggccccc cggcgtcgtc ggcagcatga cccactgcgc gggataccgg
     2161 gccgcagcgc tggcctgcag cggggacatg ctcaccgtcg ggatcgatgc tgaacctcac
     2221 cagccgctgc caaccgatgt cctgggcctc atcgccctgg ctgag
//
LOCUS       DATADN010001148         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_283189, whole genome shotgun sequence.
ACCESSION   DATADN010001148 DATADN010000000
VERSION     DATADN010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283189"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..>2108
                     /locus_tag="VHH53_14735"
     CDS             191..>2108
                     /locus_tag="VHH53_14735"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX2291408.1"
                     /translation="MAPDGRRSALALARLGFINEGAADTLARLGWWRDGQPREDAEAA
                     LWALSRTPDPNLALRTLVRLANAAGGGWAELDAALCQQPGVRARLLSVLGSSTALGDH
                     LVAKPERWRRLATSDAAAPDWADRAAEQLLAAVGADPTGPRPGTAEGTRASLTGVNAQ
                     RVLGAVYRDLIAELTAADLANVVEPEMPVLSFEQVGAALAELAVGVLHAALAVAVTEV
                     AGPDGQPPRLAVIGMGKCGGAELNYVSDVDVIFVGGAEDELSAATKLASAMMRQASTC
                     FEVDAALRPEGKTGALVRTLEGHLSYYRRWAKTWEFQALLKARAVAGDNELGDRYVAA
                     VAPLVWTAADREHFVPEVQAMRRRVTANIPAELVDRELKLGSGGLRDVEFAVQLLQLV
                     HGRTDESLRVGSTVKALAALGAGGYVGRDDAANLAASYRFLRLLEHRLQLQKLRRTHL
                     LPADDDTAALRWLARAAGMRPDGRRDAVGMLRAEYSRQSHRARRLHQKLFYRPLLEAV
                     ARVSTEAYRLTESSAKARLAALGYASPQGALGHLRALTDGVSRRAAIQSALLPVLLEF
                     LADTPDPDGGLAAYRRVSEALDETPWYLRLLRDEGAVAQRLMTLLGTSRMVADLLVRA
                     PEALRLLADPNEMAS"
BASE COUNT          279 a          693 c          793 g          343 t
ORIGIN      
        1 gtcctggcag cttgatccgg accatttgga gcaggtacac gccgatatgg cagaggtctg
       61 gcagccactg gcagcccggt tcgtggcgct ggccgctgga gaactggtac cggcggtcgg
      121 acccggggac cgcccggctc tcccgctact gggccactag cccgacggtc tctcatggca
      181 gctgagattc atggcacctg acggcaggcg tagcgcgctg gcgctcgcgc ggctgggctt
      241 catcaacgag ggcgccgcgg acaccctcgc caggctgggc tggtggcgcg acggtcaacc
      301 ccgcgaggac gcggaggccg cgctatgggc gctgtcgcgt acacctgacc ccaacctcgc
      361 gctgcgcacc ctggtgcggc tggccaatgc cgccggtggc ggctgggcgg agctggatgc
      421 cgcgctgtgc cagcagcccg gcgtgcgcgc gcgattgctg agcgtgctgg gctcgtcgac
      481 tgcgctcggg gaccatctgg tcgcaaagcc cgaacgctgg cgccggctgg ccacgtcgga
      541 cgcggccgcg cccgactggg ccgatcgggc agccgagcag ctgcttgccg cagtcggcgc
      601 ggaccccacc ggcccacggc caggcaccgc ggagggtacc cgggcgtcgc tgaccggtgt
      661 taatgcccag cgggtgttgg gagccgtgta ccgagatctg attgccgagc tgaccgctgc
      721 tgatcttgcc aatgtggtgg agcccgaaat gcccgtgctc tcgttcgaac aggtcggtgc
      781 ggcactcgcc gagctcgccg tcggggtact gcacgccgcg cttgccgtcg cggtgaccga
      841 ggtggctgga ccggatgggc agccaccccg actcgcggtg atcgggatgg gcaagtgcgg
      901 tggcgccgag ctcaattacg tctccgacgt tgatgtgatc ttcgtgggtg gtgctgagga
      961 cgagttgagt gcggccacca agttggcctc cgcgatgatg cggcaggcat cgacatgctt
     1021 tgaggtggac gcggcgctgc gcccggaagg taagaccggc gctttggtgc gcaccctgga
     1081 agggcacttg tcctactacc gccggtgggc caagacctgg gagttccagg cgttgctcaa
     1141 ggcccgcgcg gtggcgggcg ataacgaact cggcgaccgc tacgtggcgg ccgtcgcgcc
     1201 gttggtgtgg actgcggcgg accgggagca tttcgtgccc gaggttcagg cgatgcgtcg
     1261 ccgggtcacc gcgaacattc cggctgagct ggtggaccgg gagctcaagc tgggttccgg
     1321 cggtctgcgg gatgtggaat tcgcggtgca gctgctgcag ctggtgcacg ggcgtaccga
     1381 cgagtcactg cgggtcgggt ccacggtaaa ggctttggcg gctctgggcg cgggcgggta
     1441 cgtcggccgc gatgacgccg cgaacctggc ggcctcctac cggttcctgc gtctgctgga
     1501 gcaccggctg cagctacaga aactgcgccg cacccacctg cttcccgccg acgacgacac
     1561 cgcagcgttg cgctggctgg cccgcgcggc ggggatgcgc ccggatgggc gccgcgatgc
     1621 tgtcgggatg ctgcgcgcgg agtactcccg gcagtcacac cgcgctcggc ggctgcacca
     1681 gaagctgttc taccggccgt tgctggaggc cgtggccagg gtttccaccg aggcctaccg
     1741 gctgacggag tcctcggcga aggcgcggtt ggccgcgctg ggctacgcct caccgcaggg
     1801 cgcgctgggg cacctgcggg cgctcaccga cggggtttcc cggcgcgcgg cgatccagtc
     1861 cgcgctgctg ccggtgctgc tggagttcct cgccgacacc ccggaccccg acggtgggct
     1921 ggccgcctac cgccgggtgt ccgaggccct tgatgaaacc ccgtggtacc tgcggttgct
     1981 gcgcgacgag ggcgccgtgg cgcagcggtt gatgacgttg ctgggcacat ctcggatggt
     2041 cgcggaccta ctggtccggg ccccggaggc gttgcggctg cttgccgacc cgaacgagat
     2101 ggccagcc
//
LOCUS       DATADN010001149         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1505006, whole genome shotgun sequence.
ACCESSION   DATADN010001149 DATADN010000000
VERSION     DATADN010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505006"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VHH53_14740"
     CDS             complement(<1..563)
                     /locus_tag="VHH53_14740"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX2291409.1"
                     /translation="MAPPTAPIGMPPGSPGPRGGGLVKGAVVLALMVGLAAGGVGGVV
                     GYQLAGTGSGVINALDQPAVGALPAANLPGGSVEQVAQKVTPSVVQLRIRGPRVDGDG
                     SGIVLSTDGLILTNNHVVAPAVAGGELVAVLQDGRSAPVEIVGRAPSFDVAVVRAQDL
                     DGLVPAQLGSSRDVRVGQEVVAIGSPLG"
     gene            complement(800..>1921)
                     /locus_tag="VHH53_14745"
     CDS             complement(800..>1921)
                     /locus_tag="VHH53_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2291410.1"
                     /translation="VGLLERSGRGVVGGNLALGPDELAVARGEQTQSLRTDQARDLRV
                     AAVPAGSGAALIVAQGLDTTRDTLARRGLVFILVGGAGVLLAAAAGTAVAQAGLRPVQ
                     RLTEATERVARTGDLKPIAISGDDELARLTASFNSMLGALAESQERQRRLVADAGHEL
                     RTPLTSLRTNLELLAASDRPDAPSLSEQDRKEISGDVRAQVEELSTLVGDIVELARED
                     APQVVHEPLELPEVVERALARAGRRAPDVRFDVDLMPWSMLGDANALERAVLNLLDNA
                     AKWSPSDATVTVRLAPTGHGTATLNVADAGPGIAEVDLPYIFERFYRSPAARTLPGSG
                     LGLAIVAQVAARHGGKVRAGRSSTGGALMTLELPGRSST"
BASE COUNT          284 a          702 c          662 g          273 t
ORIGIN      
        1 cccaatggcg agccgatcgc caccacttcc tggccgaccc ggacgtcgcg ggagctgccc
       61 aactgagcgg ggaccaggcc gtccaggtcc tgggcccgca ccaccgcaac gtcgaaagag
      121 ggcgcgcgac cgacgatctc gaccggcgct gagcggccgt cctgcaagac ggcaacgagc
      181 tcgccaccgg ccacggccgg cgccacgaca tggttgttgg tcaggatcag gccgtcggtg
      241 ctgagcacga ttccactacc gtcgccgtct actcgaggac cgcgtatccg gagctggacc
      301 acgctgggcg ttaccttctg cgccacctgc tcgaccgacc cacccggcag gttcgcggct
      361 ggcaaggctc ccaccgcggg ctgatcgagc gcgttgatca caccggatcc agtaccggcc
      421 aactggtagc cgacgacgcc cccgacgccg cccgcggcca gccccaccat caacgccagc
      481 accacggcgc ccttgaccaa cccgccgccg cgcggcccgg gagaacctgg cggcatgccg
      541 atcggggcag tgggtggtgc cataccaccg ggctgggcgc cccaagcctc cgtgggagcg
      601 ctgtcataag gtggggaacc gtagggcctg ctccaacccg atgctgagct ctgccccggc
      661 tgccaggacg gtcccggttg accggggccc ggccaggagg ggtcagggtc gccaggccgc
      721 gccgatcgcg gcgtctcgtc ggtcatgtct ctagggtgta ctggttttct gagatgtcca
      781 tgtgaggccc ctgggggtgt tatgtggatg atcgaccggg cagttcgagg gtcatcagcg
      841 caccgcctgt cgagcttcgg ccagcccgca ccttaccgcc gtggcgggca gcgacctgcg
      901 ccacgatggc caatcccagc cccgagccgg gcagggtgcg ggcggctggc gagcggtaga
      961 accgctcgaa gatgtaggga aggtcgactt cagcgatccc tggcccggcg tcggccacgt
     1021 tcaatgtcgc cgtgccgtgg cccgtcggcg ccagccgcac ggtcaccgtc gcgtcgctgg
     1081 ggctccactt ggcagcgttg tcgagcaggt tgagcacggc gcgctccaac gcgttggcgt
     1141 caccgagcat gctccacggc atgaggtcga cgtcgaagcg gacgtccggt gcccggcggc
     1201 ctgccctggc cagcgcccgc tccactacct ccggcagctc caacggctcg tgcaccacct
     1261 ggggggcgtc ctcgcgggcc agctcgacga tgtccccgac cagcgtcgac agctcctcca
     1321 cctgggcacg tacgtcgccg gagatctcct tgcggtcttg ttcggacagt gaaggagcgt
     1381 cggggcggtc ggaggcggcc agcagttcca ggttggtgcg caacgaggtc agcggggtgc
     1441 gcagctcgtg cccggcatcg gcgaccagcc tgcgctggcg ttcctgggac tccgccagcg
     1501 cgccgagcat gctgttgaag ctggcggtga gccgggcgag ctcatcgtcg ccggagatgg
     1561 cgatgggttt caggtcaccg gtacgggcca cccgttcggt ggcttcggtc agccgctgaa
     1621 ccggtcgcag cccggcctgg gccacggcgg tgcccgcggc cgcggccagc agcacgcccg
     1681 cccccccgac caggatgaaa accagaccgc gccgggccag cgtgtcccgc gtggtgtcca
     1741 gcccctgagc cacgatcagg gcggcgcccg aacccgccgg cacggcggcg acccgcaaat
     1801 cgcgcgcctg gtcggtacgc agcgactggg tctgctcccc tcgcgctaca gcgagctcat
     1861 cgggacccag cgccaggttg ccgcccacca cgccacgccc actgcgttcg agcaggccga
     1921 c
//
LOCUS       DATADN010001150         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_126586, whole genome shotgun sequence.
ACCESSION   DATADN010001150 DATADN010000000
VERSION     DATADN010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126586"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1126
                     /locus_tag="VHH53_14750"
     CDS             164..1126
                     /locus_tag="VHH53_14750"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2291411.1"
                     /translation="MLEARHLRIVRAISDTGSLTKAAVELGLTQPALTHQLHRMERMF
                     GGTLFVRDRRGARPTPLGQHVIECARFVLPSMDSLVREARVLAGGQDTSAPQIRVAAH
                     PSPLPVAVVAALRDVLPRSQISMRTEPSGQRQIELLAAGALEVALLAEYPSLPLPRPA
                     EVVAHEVATEPLFVALSANHLLADREEICLSELAGESWALPEDADIRCAEYLRQICDQ
                     ASVTPRIAYRVTSQVARGLIARGLAVGLWQPTAPPSEGLTVRPLKGSPAELRHVIAVR
                     AGSPVSRYGAELAESARTSYWADAARSSVYRTWLGRHRPETPLF"
     gene            1405..>1634
                     /locus_tag="VHH53_14755"
     CDS             1405..>1634
                     /locus_tag="VHH53_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291412.1"
                     /translation="MSLWSQRRLRPSGERRNAVVLAIIVAMIAAVLPPAANGTHVVPI
                     LAAGPGDGTVEVPDHPEGRAFNPNQLKDIKAA"
BASE COUNT          249 a          553 c          572 g          260 t
ORIGIN      
        1 gtaacgcttc cggcgtcggc gatcgcacac acgatgcgca ggtgtcggaa ctccagctcc
       61 atggccccca gcgtaagcgc tgcgtgatcg cgtggtggca actggcaatt cccgccatcc
      121 aatggccggt gtcgccaggc cgatcggccc ataacatcgg tgcatgctgg aggctcggca
      181 cctgcgaatc gtgcgtgcta tcagcgatac cggaagcctg accaaggcag ccgtcgagct
      241 cgggctcact cagcccgcgc tcacccacca gctgcaccgg atggagcgca tgttcggcgg
      301 cacgttgttc gtgcgcgacc ggcggggtgc ccggccgacc ccgctcggcc agcacgtcat
      361 cgaatgcgcc cggttcgtcc tgccatccat ggactcgctg gtgcgcgagg cgagggtgct
      421 ggcgggcggt caggacacgt ccgccccgca gatccgggtc gccgcgcacc cgtccccgct
      481 accggttgcg gttgtcgcgg cgctgcggga cgtcctgccg cgcagccaga tcagcatgcg
      541 caccgagccg tccggccagc gccagatcga gttgctcgcc gcgggtgcgc tggaagttgc
      601 gctgctggcc gagtatccga gcctcccgct gccgcggccg gccgaggtgg tagcgcacga
      661 ggtggcgacc gagccactgt tcgttgcgct gtcggccaac cacctgctcg ccgaccggga
      721 ggagatctgt ctgtccgaac tggccggtga gagctgggcg ctgccagagg atgccgacat
      781 ccggtgcgcc gagtacctgc gccagatctg cgaccaggcg agtgtcacgc ccaggatcgc
      841 ctaccgggtg acttcgcagg tggcccgggg tctgatcgcg cgcgggttgg cggtcgggct
      901 gtggcagccg acggcacctc ccagcgaggg tctgactgtc cggccactca agggttctcc
      961 cgccgagctg cggcacgtga tcgcggtgcg ggccggcagc ccggtgtcgc gctacggggc
     1021 cgagctcgcc gagtccgccc ggaccagcta ctgggcggac gccgcgcggt cgtcggtgta
     1081 ccgcacgtgg ttgggccggc accgcccgga gactccgctt ttctgagtgg actcgccacc
     1141 gccgctcaac tggcgatgca ggaattagcc cggcacaaga tcaaagcgtc gtcgccctgt
     1201 gctaccgccg gtcgggccga aggtcaaccg ccgaatggtc aggtggttgg attccgtttg
     1261 cgcacaacgg ttgagccatt gatcgcatcg gctaacggcg cggttacgag caggtgaaga
     1321 gatcacgcca agtcccgccc gtaggtaatc actaaccatg acgcggcgat tacacgcggg
     1381 ctactgcgcg tgagaggtgg ggctatgagc ctctggagtc agcgtcgtct ccgtccgtcc
     1441 ggcgaacgcc gcaacgcggt ggtgctggcg atcatcgtgg ccatgatcgc agccgtcctg
     1501 ccgccggccg cgaacggcac ccacgtggtg ccgatcctgg cggccgggcc gggtgatggg
     1561 acggtggagg tccccgacca tcccgagggc agggcgttca atccgaacca gctcaaggac
     1621 atcaaggcgg ccga
//
LOCUS       DATADN010001151         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_598939, whole genome shotgun sequence.
ACCESSION   DATADN010001151 DATADN010000000
VERSION     DATADN010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598939"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1460
                     /locus_tag="VHH53_14760"
     CDS             246..1460
                     /locus_tag="VHH53_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX2291413.1"
                     /translation="MSTSTGVQFLDVLDPGFDFGSTEVRLAQDRNWYADSPIGIIVLR
                     YAEAQELLRDQRLNHNSERYLEMNGVVDGPIYDWFVPMIVSHDGEHHRRLRGLVNKAF
                     TPRMISNLRPFIRAQAEQLADRLESADEIDFVRDFADKLPLAVMCELLGVPAEDYADF
                     RVWTSDIGLVFGIALGEDIRQRVEAAVVGLNGYVDSLLEAKRADPGDDLISALLAIQR
                     TDESRVSLAELQNLIVTLVFAAHDTTRHQLANAMATFAEYPEQWAALGRQPELADQAV
                     EEVMRWNPSSTTLYRFAAEDFDYRDLHLTTGTFLSIGVAIAQRDSRVFHNGHTFDITV
                     QREAPPLQFGGGPHHCLGSALARAELSEALPVLAPRLGPPTITGPVTWRPAIGIRGPY
                     QLPLRFATPRQH"
BASE COUNT          275 a          563 c          492 g          277 t
ORIGIN      
        1 tcaccaggcg gggtgaccac cgagagcgtc ccggacatcg gtgttggcga gcagatctgg
       61 ccgttccgcc ggggtgagct ccgtcgtctg gttgatcaat tcatgatccc agaagtagaa
      121 ttgatctaca tttttacccc gcttcacaag gtgcggatcg acccctttcg tgcgggcgat
      181 ccatgcggca tcctgggtag gcatccttcg ccgtcggcga gcaccctcat gcgcgggagg
      241 cctatatgtc gacatcaact ggcgtgcaat ttcttgatgt gctcgacccg ggtttcgatt
      301 tcggttcgac cgaggtcagg ctggcccagg accggaactg gtacgccgac agccccatcg
      361 ggatcatcgt gttgcgctac gccgaggccc aggagttgct gcgcgaccag cggctcaacc
      421 acaacagcga gcgctatctc gaaatgaacg gagtcgttga cggcccgatc tacgactggt
      481 tcgtgcctat gatcgtcagc catgacgggg agcaccaccg ccggttgcgc ggcctggtca
      541 acaaggcgtt caccccgcgt atgatcagca acttgcggcc gttcattcgc gcgcaggccg
      601 agcagctggc cgaccggctg gagtcagccg atgagatcga tttcgtgcgc gatttcgccg
      661 acaagcttcc gctggccgtc atgtgcgagc tgctcggggt accagccgag gactacgccg
      721 atttccgggt atggaccagt gatatcgggc tggtgttcgg catcgccctc ggcgaggata
      781 tccgccagcg ggttgaggcc gcggtcgtgg gactgaacgg ctacgtcgac tcgttgctcg
      841 aggccaagcg tgccgacccc ggcgacgatc tcatctcggc gctgctggcc atccagcgca
      901 ccgacgagtc ccgggtcagc ctggcggagc tgcagaacct gatcgtcaca ctggtgttcg
      961 ccgcccacga caccacccgg caccagctcg ccaacgcgat ggcgaccttc gccgagtacc
     1021 ccgaacagtg ggccgcgctg ggccgtcagc ccgagctggc cgaccaggcc gtcgaagagg
     1081 tcatgcggtg gaacccgtcg agcaccaccc tctaccgctt cgccgccgag gacttcgact
     1141 accgcgacct gcacctcacc accgggacgt tcctctccat cggcgtggcg atcgcgcagc
     1201 gcgacagccg ggtgttccac aatggacata cgttcgacat caccgtccag cgcgaggcgc
     1261 cgccgctgca gttcggcggc gggccacacc actgcctcgg ttccgcgctc gccagagccg
     1321 aactcagcga ggccctaccc gtactggccc cccgactcgg cccaccgacg atcaccggcc
     1381 cggtcacctg gcgcccggcc atcggcatcc gcgggcctta ccagctgccg ctgcgcttcg
     1441 ccacaccgcg ccagcattga ggatctcagg acagctgacc agcctcgtca gcgagcctga
     1501 cgagcatcag gccgtcctcg actccccgct gccggacgct cccagtcaca cctggactac
     1561 ctgccctcga ccggtgcagc tgcaccagcg gtacgggtaa ccggaag
//
LOCUS       DATADN010001152         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_884962, whole genome shotgun sequence.
ACCESSION   DATADN010001152 DATADN010000000
VERSION     DATADN010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884962"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHH53_14765"
     CDS             <1..318
                     /locus_tag="VHH53_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX2291414.1"
                     /translation="RADFDLDSIDDLRMAVDDVCAMLVRIAARDAALTCSFTVRTNRI
                     ELTAEIDADALADPVPTTSFGWRVLESLADEVSVVHLPGVQGEGGKVCITLAKGPVSV
                     PQT"
     gene            315..1109
                     /locus_tag="VHH53_14770"
     CDS             315..1109
                     /locus_tag="VHH53_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX2291415.1"
                     /translation="MTSPGSSAAATRDGEYAHLIPLQRRYAELATDDPDRRRLREQLI
                     NGYRPMAEHIARRFAGRGEPLDDLVQVATMGLINAVDRFEPDRGSDFFSFAVPTITGE
                     VRRYFRDHGWSTRVPRRLKDLHIAIRGTLAELSQQLGRAPRPSEIAARLDLPLADVIE
                     GLRAGDAYRSSSLDEMLGSGEGSATLGELMGGLDGDLALIDDREALRPLLADLAPRER
                     TILTLRFFRQMTQTQIAEQVGISQMHVSRVLRQTLAELRERMSAPD"
     gene            1156..1494
                     /locus_tag="VHH53_14775"
     CDS             1156..1494
                     /locus_tag="VHH53_14775"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX2291416.1"
                     /translation="MRDEEFITEVQTRAGLSTRAEAERVTQATLAVLGEHLPDSVAGA
                     AAAALPERLGEQLWGRAARSQATAAQAGRLSRGVERGEGSWPGDNTSADADIDAGDPT
                     ADRSPSGGAG"
BASE COUNT          274 a          489 c          535 g          252 t
ORIGIN      
        1 cgggcggatt tcgacctgga ctcgatcgac gacctgcgaa tggcagtcga cgatgtctgc
       61 gccatgctgg tgcggatcgc cgccagggat gccgctctga cctgcagctt taccgtccgg
      121 acgaatcgga tcgagctgac cgcggagatc gacgcggacg ccttggcaga tccggtgccg
      181 acgacgtctt tcgggtggcg ggtattggaa tccctggccg atgaggtcag cgtggtgcac
      241 ctgcctggtg tccaaggcga aggtggcaag gtctgcatca ccctggccaa gggccccgtg
      301 tccgtgccgc agacgtgact tcgcccggtt cgtcagccgc cgccactcgc gacggcgaat
      361 acgcccatct catcccgttg caacggcgct atgccgagct ggcaaccgac gatcctgacc
      421 gccggcggct acgcgagcag ctgatcaacg gttaccgtcc gatggccgaa cacatcgcac
      481 gccggttcgc cggtcgcggc gagccgttgg atgacctggt tcaggtcgcc accatggggc
      541 tgatcaatgc cgtggaccgg ttcgagccag accgcggctc ggatttcttc tcctttgcgg
      601 tgcctacgat cactggcgag gtgcgccgat acttccgcga ccatggctgg tcgacgcgag
      661 tgccgcgccg gctcaaagat ctacacatag ccatccgggg cactctggcc gagctgtccc
      721 agcaactcgg tagggcgccg cgacccagtg agatcgccgc ccggctggac ctgcccctcg
      781 ccgacgtgat cgagggactg cgggctggag atgcctaccg aagctcgtct ttggacgaga
      841 tgctcggctc tggcgaggga agcgcgaccc tcggtgaact catgggtggt ctggacggtg
      901 atctcgcact catcgatgac cgggaagcgc tgcgcccgct gctggctgac cttgcgcccc
      961 gggaacggac aattctcacg ctacggttct tccgtcaaat gacgcagact cagatcgctg
     1021 agcaggtcgg catctcgcag atgcacgtct cgcgcgtgct ccgccagaca ctagcggagc
     1081 tacgggaacg catgtcggcc ccggactgaa ccggactgaa ctgagaagag cgctgaattg
     1141 ggcaggaggg atcagatgcg cgacgaggag ttcatcacag aggtgcaaac aagggctggg
     1201 ctgagcactc gtgccgaggc cgaacgcgtc acccaggcga cgttggccgt tctgggcgag
     1261 catctaccgg acagtgtggc cggcgcggca gccgccgcct tgccggagcg gcttggtgag
     1321 cagctgtggg gccgagcagc gcgctcacaa gcgaccgcag cacaggcagg gagactgagc
     1381 aggggagtgg agcgtggcga gggtagctgg ccaggtgaca acaccagcgc cgacgccgat
     1441 atcgacgccg gggaccccac ggcagaccga tcgcccagcg gtggcgccgg ctgacctgta
     1501 gcgtcgaggg cgaagtgaac tgtgtggcgc gggaccggtg tggaccccac
//
LOCUS       DATADN010001153         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_283746, whole genome shotgun sequence.
ACCESSION   DATADN010001153 DATADN010000000
VERSION     DATADN010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283746"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1296)
                     /locus_tag="VHH53_14780"
     CDS             complement(334..1296)
                     /locus_tag="VHH53_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX2291417.1"
                     /translation="MLTTSARLLGLLSLLQTRPEWSGAELAERLGVSTRTVRRDIERL
                     RDLDYPVRSTRGVAGYRLGAGAALPPLLLDDEEAVAVAVGLRTAAAGVSGIEEASLGA
                     LAKLEQVLPTRLRRRVNAVQASTLPVPGDGPGPRVDPAVLAVLAAACRDRERLRLAYR
                     DYQGSVGRRALEPYRLVSWGRRWYLVAWDLDREGWRILRVDRVEPATVTPTGQRFTPR
                     ELPEGGDLAAYVSRRVSAAAWRHYARVRVHAPAEQIRRQIPAAAGVVEPVDQATCLLH
                     TGADTLQTLAVYLGMLGADFEVGEPPELVAHLRGLADRYRRATT"
     gene            1393..2541
                     /locus_tag="VHH53_14785"
     CDS             1393..2541
                     /locus_tag="VHH53_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX2291418.1"
                     /translation="MPDTAIRPFRIDVPQADLDDLTERLARTRWANELPLDTSRPQTG
                     PVPPGWEYGVPVSYVKDLVEHWQRHYDWRAWQTKLNEYPQFTTTIDGQNIHFLHVRSP
                     EPAAIPLILTHGWPNTFLEYVDLIGPLTDPRAHGAEATHAFHVVVPSLPGFAFSGPTT
                     EPGWDAHRTARAWTELMRRLGYDRYGAHGNDAGAIVAPLQGRVDREHVIGVHVNQIFS
                     FPSGDPDELEGLTPEELGYLSFLQGFVERAVHDKVQAAQPQTIAHALADSPAGQIAWS
                     GQLLGNALSKDQILTNVAIYWLTNTAASAARFYYENQHADHPTEPTTIPMGLASFAFD
                     FRPIRRFAERDHANIVSWSEFDRGSHWAAHDAPDLLVADLRQFFARFR"
     gene            2903..>3190
                     /locus_tag="VHH53_14790"
     CDS             2903..>3190
                     /locus_tag="VHH53_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291419.1"
                     /translation="MAGGLAWALWTLVLLGLAVTPWLNQQARQAGRSDLGSDANTVVD
                     GLVAVSAATVGTVLARQGAACEWGLGIGMAAAYRWPMTGNRERLALASFLAS"
BASE COUNT          507 a         1193 c         1052 g          438 t
ORIGIN      
        1 cgctggccac ggccttggcc gcccgggcga acgcatcgaa tagggctgtc ttgaccgccc
       61 ggtcagccat gtacctcctc accggcccat tcaagtgatc acttgacaac ttgaataagt
      121 ggcaggatag cccctcggcg gcaggtgggc caaccagacg gaggcagaca catggccacc
      181 ccgccaacca acgccagctg gtggccgttt ctgaacttga tcgagcgctc ccccaacacc
      241 atccggggtc atgcccacga cctcaaggat tggttcgcct tcctcccgct ctcccacctg
      301 ggcgtcggcg gacagcgtcg gatcgcgacc ggctcaggtg gtggctcggc ggtagcggtc
      361 ggccaggccg cggaggtgcg cgaccagctc gggcggctca ccgacctcga agtcggcacc
      421 cagcatgccc aggtagacgg ccagcgtctg gagggtgtcg gcaccggtgt gcagcaggca
      481 ggtcgcctgg tcgaccggct ccaccacccc ggccgccgcc gggatctgtc gccggatctg
      541 ctcggctggt gcgtgaaccc ggactcgggc gtagtgacgc caggcggcgg ccgacacccg
      601 ccgcgagacg taggcagcca ggtcgccacc ctcgggcagc tcccgcgggg tgaaccgctg
      661 gccggtcggg gtcaccgtgg ccggctccac ccggtcgacc cggaggatcc gccagccctc
      721 ccggtccagg tcccaggcca ccaggtacca gcgccgcccc cagctcacca gccggtaggg
      781 ctccagggca cgccggccca ccgagccctg gtagtcccgg taggccaagc gcaatcgctc
      841 gcggtcacgg caggcggcgg ccagcacggc cagcacggcc gggtccaccc ggggaccagg
      901 gccatcgccg gggaccggca gcgtggacgc ctgcacggcg ttgacccggc ggcgcagccg
      961 ggtgggcagc acctgctcta gcttggccag ggcaccaagg gaggcctcct cgatgccact
     1021 cacgccagcg gcggccgtgc gcagcccgac cgccaccgcc acggcctcct cgtcatccaa
     1081 cagcagcggg ggcaaggccg ctcccgcccc gagccggtag ccggccacgc cccgcgtgga
     1141 gcgcaccgga tagtccaggt cgcgcagccg ctcgatgtcg cgccggacgg tccgggtgct
     1201 caccccgagc cgttcggcca gctcggcccc ggaccactcc ggccgcgtct gcagcagcga
     1261 cagtagcccc aggagccgag cggaggtcgt cagcatggat cccagtctgc cagccaccta
     1321 ggacagttcc tgacccaact ggctcctagg atggcgcgca ttccgccgac caaccggaaa
     1381 ggagccaccc cgatgcctga caccgccatc cggccgttcc gcatcgacgt gccccaggcc
     1441 gacctggacg acctgaccga gcggctggcc cggacccgct gggccaacga gctgcccctc
     1501 gacaccagcc gtccgcagac aggcccggtt ccacctggct gggagtacgg cgtcccggtg
     1561 agttacgtca aggacctggt cgaacactgg caacgccact acgactggcg cgcatggcaa
     1621 acaaagctca acgaataccc acagttcacc accacgatcg acgggcagaa catccacttc
     1681 ctgcacgtcc gctcccccga gccggccgcc atcccgctga tcctgaccca cggctggccc
     1741 aacaccttcc tggagtacgt ggacctgatc gggccgctca ccgacccgcg ggcgcacggg
     1801 gccgaggcca cccacgcctt tcacgttgtc gtcccctcgc tgcccggctt cgccttctcc
     1861 gggccgacca ccgagccggg ctgggacgcc caccgcaccg cgcgggcgtg gaccgagctc
     1921 atgcggcggc tcggctatga ccggtacggt gcccacggca acgacgccgg cgccatcgtg
     1981 gcccccctcc agggacgcgt cgatcgcgag catgtcatcg gggtgcacgt caaccagatc
     2041 ttctccttcc cctcgggcga ccccgatgag ctggagggcc tgaccccgga ggaactgggc
     2101 tacctgagct tcctgcaggg ctttgtggag cgagcggtcc acgacaaggt gcaggccgcc
     2161 cagccgcaga ccatcgccca cgcgctggcc gactccccgg ccgggcagat cgcctggagc
     2221 ggccagctgc ttgggaacgc cctgagcaag gaccagatcc tcaccaacgt ggccatctac
     2281 tggctgacca acaccgccgc ctcggcggcc cgcttctact acgagaacca gcacgccgac
     2341 cacccgaccg agccgaccac gatccccatg ggcctggcca gcttcgcctt cgacttccga
     2401 ccgatccgca ggttcgccga acgtgaccac gccaacatcg tctcctggag cgagttcgac
     2461 cgcggcagcc actgggccgc ccacgacgcc cccgacctgc ttgtcgctga cctgcgccag
     2521 ttcttcgccc gattccgctg accacacggc gccagcgccc ctactgcacc gccagcaacg
     2581 ccagaaatcc ctctgacgtg cagttttcag cccgaggtcg gattcggacc gacgaccttc
     2641 tgattccgag acggatgctc ggcgtcgacc tggacggctc cagacggatc ggatgacaag
     2701 gcgagcacga ggtcacgcgc tgttcattgg caggagcctc gggcgggcct aggccggtct
     2761 gccgtccgca acgttaccgg gacgcaggcc ggccagactg gtggtcatcc gcaggcttgg
     2821 gaggtggcag ggctggtggt gcgctgggtg cagcggggac catggccgtg acttctgccg
     2881 gatggcttgt ccggaggcgc cggtggccgg cgggctggcg tgggcgctgt ggacgctggt
     2941 cctgctcggc ctggccgtaa cgccgtggtt gaaccagcag gctcgccagg ccggccgctc
     3001 ggacctgggg tcggacgcca acaccgtcgt cgacgggctg gtagcggtga gcgcggccac
     3061 ggttgggacg gtgctggcca ggcagggcgc agcttgcgaa tgggggttgg ggatcgggat
     3121 ggccgcagca taccgttggc caatgaccgg caatcgggag cggctggcgc tggccagctt
     3181 cctggccagc
//
LOCUS       DATADN010001154         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_126933, whole genome shotgun sequence.
ACCESSION   DATADN010001154 DATADN010000000
VERSION     DATADN010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126933"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..864)
                     /locus_tag="VHH53_14795"
     CDS             complement(16..864)
                     /locus_tag="VHH53_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291420.1"
                     /translation="MPELKADTPMQKVVLPGLVELTLGNVVVPDRFHPHRIAGFVTRL
                     EDVPASSSSELARRYGLDQVAGWPVTDELYVLRFYAHSPQLYIAPFERSVYQVDLLEL
                     PAGTELWQIDAQGDEQRVGAYLNRQVGWVSTARPMLGPASWNRPPVPLRPTVRRGLVA
                     QYRGDDFDADFGPRPGEVTLHTLPGKRPPKEFTEQAGAYTLAVMICELDSLELVRWRG
                     GWNGLPVELVESGPEYVVVHYIGENGPRAAEFGLAEVDYRVWRGAVPHSELTDVAEQR
                     VELHSS"
     gene            complement(1163..1636)
                     /locus_tag="VHH53_14800"
     CDS             complement(1163..1636)
                     /locus_tag="VHH53_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2291421.1"
                     /translation="MGRGTDEPRWLNAAEMRAWRAYVVGKAVLDSRLHQDLQQQHQLT
                     LADYELLVRLSEAPCGQARMSILAEQVASSKSRISHQIGRMERAGLVRRQECPSDGRG
                     VIAELTTRGWQVLRSAAPTHVRGVRDNLIDLLSDDERTVLAEVFERVMARQREED"
     gene            complement(1682..2782)
                     /locus_tag="VHH53_14805"
                     /pseudo
     CDS             complement(1682..2782)
                     /locus_tag="VHH53_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
BASE COUNT          477 a         1043 c          909 g          436 t
ORIGIN      
        1 gcctgccagg cccactcagg aggagtggag ctccacgcgc tgctcggcca cgtcggtcag
       61 ctcgctgtgc gggaccgctc cgcgccatac ccggtagtcg acctcggcca gaccgaactc
      121 agctgcccgt ggaccgttct ccccgatgta gtggaccacg acgtattcgg gaccggactc
      181 caccagttcg accggcaagc cgttccaccc gccgcgccag cgcaccagct ccagcgagtc
      241 gagctcacaa atcatcactg ccaacgtgta ggcgccggcc tgttcggtga actccttcgg
      301 cgggcgcttg ccgggcagcg tgtgcagggt cacctcgcca ggacgaggac cgaagtcggc
      361 gtcgaagtcg tcaccgcggt actgcgcaac caggccgcgc cgcacggtgg gccgcaacgg
      421 caccggcgga cggttccagc tggcaggacc cagcattggt cgcgcggtgc tcacccagcc
      481 gacctgccgg ttcaggtagg cgcccacccg ctgctcatcg ccctgggcgt cgatctgcca
      541 cagctcggtg ccagcgggga gttcgagcag atccacttga tagacgctgc gctcaaaggg
      601 cgcgatgtag agctgggggc tgtgcgcata aaagcgcaat acgtacagct cgtcggtgac
      661 cggccagcca gccacctggt ccaggccgta gcgccgggca agctcggacg acgaactcgc
      721 cggaacgtcc tctagccggg tcacgaaccc ggcgatccgg tgtgggtgga accggtccgg
      781 caccacgacg ttgcccaagg tcagctccac cagcccaggc aacacgacct tctgcatcgg
      841 cgtgtcggcc ttgagctcag gcacctgacc aggcaccctc agccggggcg gcggcggggc
      901 gaggcggtcc tcgggcggcg cgcggtccac accacgcgct gggccttcca ctgtgctgtt
      961 cgctgaaggg ccgttcaccg aagggccgtt cactcgggca ccgttcgctg gggcgctgtc
     1021 ggcccctaag tcgttggctg ctacccctgc tgccggatct gtcatgaacc ccaccactcg
     1081 atgccgtcag gccccgcgcg taccccgccc gggttggttg gggaacctac tacgccattc
     1141 gtgcgcggtt cgaccggagg ggttagtcct cctcccgctg gcgggccatg acgcgctcga
     1201 acacctcggc cagcacggtt cgttcgtcgt cggagagcaa atcgatcaga ttgtcccgaa
     1261 caccccggac atgggtggga gccgcgctgc gcagtacctg ccagccgcga gtcgtgagct
     1321 cagcgatcac cccgcgcccg tcgctggggc attcctggcg gcgcactagg ccggcccgct
     1381 ccatccggcc gatctggtgg gagatccggc tcttcgacga cgcgacctgc tcggccagga
     1441 tgctcatccg agcctgcccg cagggagcct cggagagccg gaccagcagc tcgtagtcgg
     1501 ccagtgtcaa ctggtgctgc tgctgcagat cctggtgcag ccgtgaatcg agcaccgcct
     1561 tgccgaccac gtacgcccgc cacgctcgca tctcggcagc attgagccag cgcggttcgt
     1621 cagtccccct gcccatccct gcaggctaac ccaccacacc gaagtctgat catgatctgt
     1681 atcaaccctc ccggacgatg ctgtgggcgc tcaattctgc tgggcgggaa tagccggcca
     1741 gagtcagcat gaggtcgaga tcaccgagga ccgcgcgcag cacatgctct accccactgc
     1801 gcccggccag gcccagcccg tagacccacg gccgcccgag cagcacggcg tgcgcgccga
     1861 gcgccagcgc gatcagcacg tcggctccgc acctgatccc ggagtctagg agcaccggcg
     1921 cccgcccgtc gacggctgct accacgtcag gcaggcagtc cagcgccgct cgagccccgt
     1981 cgacctgacg gccaccgtgg ttggacacca ccatcccgtc ggcaccgtgc tccaacgcag
     2041 cccgggcatc gtcgggatgg cagatcccct tgatcagaat cggcaactgg gtccactcgc
     2101 gcagcagcga caggtctgac caacccagcg ttggattgcc gaacaactcc atccaggtcc
     2161 gcacggcctg ctgcatagcc tgcgccgatt cctcgggtgg cgcttcgagc cgggcgcgga
     2221 acaccggatc ggacaggtag ttggtgatgt gcttggcatg caagaaaggc aggtgcccca
     2281 gctgtaggtc gcgcggtcgc cagctcaacg accaggtgtc cacggtgacc acaatggctt
     2341 ggtatccggc cgcctcggct cggccgacca gcgactcggc cagcgccggg tcacgcggcc
     2401 agtacagctg aaaccagcgg ctagcatccc cagtagcggc tgcgacgtct tccagcgtgg
     2461 ccgatcacac tgttgacagc acgctgacca gtcccagttc tgcggcggcg cgggcgaccg
     2521 cgaggtcagc gtccggatgc acgaggccca gtgccccgat cggtgccgtg agcaccggcg
     2581 ccgtcattgg tgtgcccagc acggtggtgg acagatcccg ctcggtgacg ccgcgcaaca
     2641 tccgcggcac gatccgccag cgggcgaacg cctcgcggtt cgccgcgacg gtccgctcag
     2701 tggacgcccc accggccacg tacccgaacg cttcagccgg cagtacgcgc tgcgccgcag
     2761 cttccagccc ctcggcggtc atcggcaact ccggtcgctg cccggccaca ccacgcagat
     2821 aaatttccct tgacacgtca ccgaaatggg ccacattggg cacca
//
LOCUS       DATADN010001155         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1055222, whole genome shotgun sequence.
ACCESSION   DATADN010001155 DATADN010000000
VERSION     DATADN010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055222"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VHH53_14810"
     CDS             complement(<1..690)
                     /locus_tag="VHH53_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX2291422.1"
                     /translation="MTVRGVLRRAVEVYRDEPQTSQWLRQQLYRLDEPLRVAIAGKVK
                     AGKSTLLNALVGEQIAPTDAGECTRVVTWYCDGRSPKIVMHPKEGPPTSLPVNRQDGA
                     LTIDLQGTSAEALNRLVVHWPAQSLRTATLIDTPGIASISIDNSQRTLTFLTPEDDSP
                     AEADAVVYLMRHLHATDAKFVESFRDQGVGRATSVNTVAVLSRADEIGGGRVDAMFSA
                     KTIAQRYRKDPT"
     gene            complement(729..>2480)
                     /locus_tag="VHH53_14815"
     CDS             complement(729..>2480)
                     /locus_tag="VHH53_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX2291423.1"
                     /translation="RLQHARARLLDEQVRVLVVGEFKQGKSLLVNGLVKALVCPVHDD
                     MATSVPTVVRHADTPVAALVRLLDSERDDLSNRRTERVEIPIDKLAEHVSENGNPGNK
                     EGWSYAEVGLPRRILTGGLELVDTPGVGGLNSVHGAATMAMLPSADAVLLVSDASQEY
                     TAPELEFLRQAAKVCPNVACVLTKTDLYPEWRRIAELDRAHLATVDIDAQLMAVSSTL
                     RVQSVQVQDADLDAESGFPALITYLREHVVGQADLLARRSTVHDVLVVTDQLAAGLRA
                     EQAAQLNPEAAQGVIKQLTEAQQRSAALKERTARWQHTLNDGVADLNADIDYDLRDRM
                     REIVRLAEEEVDRSGDPTKTWDQLSTWVEQEVSSAASANLVWATQRARWLATQVADHF
                     AEDENRVLPKLRTEVADALGAVRTMQLREVESFGIGRKALTGLRGGYVGVLMFGMLGT
                     LVGLSLINPFSIGAGLLLGGKTITDERRRITSRRQNEAKTAVRRYIDDVIFQASKESR
                     DMLRRVQRDLRDHFTTQAEETNRSLSDSLRAAEKSVKTTKGERERRLTEIKAELERLE
                     TLQQKVRTLLPQATPAR"
BASE COUNT          396 a          827 c          798 g          459 t
ORIGIN      
        1 cgtggggtct ttgcgataac gctgcgcgat ggtcttcgcc gagaacatcg catcgacccg
       61 gccgccgccg atctcgtcgg cccgcgacag caccgcgacg gtgttcaccg acgtcgcgcg
      121 gcccacaccc tgatcccgga acgactccac aaacttggcg tccgtggcgt gcaggtgacg
      181 catcagatag accaccgcgt cggcctcagc gggagagtcg tcctcggggg tgaggaaggt
      241 gagagtgcgc tgcgagttgt cgatcgatat cgacgcgata cccggcgtgt cgatcaaggt
      301 agcagtgcgc aagctttgcg caggccaatg caccaccagg cggttcagcg cctcggcgga
      361 ggtgccctgt agatcgatgg tgagtgcccc gtcctgccgg ttcaccggta acgaggtagg
      421 cggaccctcc ttgggatgca tcacgatctt ggggctgcgc ccgtcgcagt accaggtcac
      481 gacccgggtg cattccccgg cgtcggtcgg tgcgatctgc tcgcctacca gcgcgttgag
      541 cagtgtcgat ttgccggcct tgaccttgcc tgcgatcgcg acccgcagcg gctcgtcgag
      601 tctgtacaac tgctggcgca gccattgcga agtctgcggc tcatcacggt agacctcgac
      661 cgctcggcgg agcacgccgc ggacggtcac ggacaggcta cgttgacctc ccacccgcac
      721 cggagcgctc atcgagccgg agtcgcttgg ggcagcagcg tgcggacctt ctgctgcaag
      781 gtttccaagc gctccagttc ggctttgatc tcggtcaacc ggcgctcccg ctcccccttg
      841 gttgtcttga ccgacttctc ggcggcccgc aacgagtcgc tcagcgaccg gttcgtctcc
      901 tcggcctggg tagtgaaatg gtcccgcagg tcacgctgca cccgccggag catgtctcgg
      961 gactccttgc tcgcctggaa gatcacgtcg tcgatgtatc gacgtaccgc cgtcttcgcc
     1021 tcgttctgcc gtcggctggt gatacggcga cgctcatcgg tgatggtctt gccacccagc
     1081 agtaggcctg cgcctatcga gaatgggttg atcagcgaca atccgacaag agttcccagc
     1141 attccgaaca tcagcacccc gacgtaacca ccccgcagac cggtcagcgc cttgcgtccg
     1201 atgccgaacg attcaacctc gcgcagctgc atggttcgca ccgcgcccag cgcatcggcg
     1261 acctcggtcc gcagtttggg cagtacccgg ttctcatcct cggcgaagtg gtcagcgacc
     1321 tgcgtggcca gccagcgcgc ccgctgggtg gcccagacga ggttcgccga ggccgccgag
     1381 gaaacctcct gctcgaccca ggtcgacagt tggtcccagg tctttgtcgg gtcaccgctg
     1441 cgatcgactt cttcctcggc caatcggacg atctcgcgca tccggtcccg caggtcgtag
     1501 tcgatgtcgg cgttgaggtc ggcgacgccg tcattgagcg tgtgttgcca gcgtgcggtt
     1561 cgttccttca gtgccgcgga gcgctgctga gcctcggtca gctgcttgat cactccttgg
     1621 gcggcttcgg gattcaactg ggccgcctgc tcggcgcgca gcccagcggc aagctgatcg
     1681 gtgaccacga gcacatcgtg caccgtcgac cggcgcgcca gtagatccgc ctggcccacg
     1741 acatgctcac gcagataagt gatcagcgcc gggaaaccgg attcagcgtc aaggtcggcg
     1801 tcctgaactt ggactgactg cacgcgcagg gtggaggaga cagccatcag ctgcgcatcg
     1861 atgtccaccg tcgccaggtg tgcccggtcg agttccgcga ttcgccgcca ctctggatac
     1921 aggtcggtct tggtgagcac gcacgccacg ttggggcaca ccttggcggc ttggcgcagg
     1981 aactcgagct caggtgcggt gtattcctgg gaggcgtcgg acaccagcaa gacagcgtcg
     2041 gcggagggca acatggccat cgtcgccgca ccgtgcacgg agttcagtcc accgactcct
     2101 ggggtgtcga ccaactccag cccaccggtc aggatgcggc gcgggaggcc gacctcggcg
     2161 tagctccacc cctccttgtt gccgggattg ccgttctccg agacgtgctc ggcgagcttg
     2221 tcgatgggta tctcgacgcg ctcggtgcgc cggttgctca gatcgtcacg ttcagagtcg
     2281 aggagacgga ccagggcggc cactggggtg tcggcgtggc gaaccacggt gggcaccgag
     2341 gtggccatgt cgtcgtggac cgggcacacc agtgccttca ccaagccgtt gaccagcaag
     2401 ctcttgcctt gtttgaactc gccgaccacg agcacccgca cctgctcgtc cagcagccgg
     2461 gcgcgggcat gctgcagccg
//
LOCUS       DATADN010001156         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_885241, whole genome shotgun sequence.
ACCESSION   DATADN010001156 DATADN010000000
VERSION     DATADN010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885241"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VHH53_14820"
     CDS             complement(<1..349)
                     /locus_tag="VHH53_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291424.1"
                     /translation="MTSVDDLTQGLDQLRDSIAQTARAAPQVARQEIQREGRAYFRGA
                     VVASVITSLLIGVPLLMLNRRRAENTAEDQVRAAQFRAASQDIQRLAQGAYDLGMVAN
                     AELTRRGLATVSIP"
     gene            complement(830..1246)
                     /locus_tag="VHH53_14825"
     CDS             complement(830..1246)
                     /locus_tag="VHH53_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEX2291425.1"
                     /translation="MAQKTIVTLIDDLTGEEAEDITTVEFALEGITYEIDLADGNAAK
                     LRDNLARYVAAARKTSGRRPGTRGADRSGGRTAGNGSVARSGYNRDTLKAIREWAKQN
                     GHNVSDRGRLPLNVVQAWEAKHQGTTNSGKVPAFSG"
     gene            complement(1501..>1869)
                     /locus_tag="VHH53_14830"
     CDS             complement(1501..>1869)
                     /locus_tag="VHH53_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2291426.1"
                     /translation="LVPQRRSAGMTAVAWRTSAGTAARLPVARATNLVRTLKDCAASG
                     LMVVGLDAGGSMSLDSFPWATGPLALVLGSEGRGLSRLVRETCDASVSIPIAGPVESL
                     NASVAAGVVLAEVARLRRAG"
BASE COUNT          350 a          635 c          566 g          318 t
ORIGIN      
        1 cgggaatcga taccgtcgcc aggccgcggc gggtgagttc ggcgtttgcc accatcccga
       61 ggtcgtatgc accctgcgcg agccgctgaa tatcctgcga cgcggcccgg aactgggccg
      121 cgcgcacctg atcctccgcg gtgttctcag cgcgacggcg gttcagcatc aacaacggaa
      181 caccgataag caaggatgtg atcactgagg ccaccaccgc gcctcgaaag taagcgcgtc
      241 cttcgcgctg gatttcctgc cgggctacct gcggggcggc tcgggcggtt tgcgcgatag
      301 agtcccgcag ttgatccaat ccctgagtga gatcatccac gctggtcatg ggattctctc
      361 atctgaagca cctcaagccg tccgactcga gcctgcaggt cggcaatcgc ggactggatg
      421 agagtgaccg cgtcgctggc ctgttgcgcg atcaccatgg gcgtcggcgg tacaaaacta
      481 ggccgacctg atttgaagcg ctcaacgatc accggaccaa ccacagccgt caccacgatt
      541 ccgatcaggg agatggccgc gatagtgatc gtcagccaag ctggcccccc agcttgaaca
      601 gttcctccgt tccgccgccc tgcactgact tatgcccagc gcaggtgacg agagcgactc
      661 atacccaacc ggccccggca tacgccgcta ctcccccaac ctgacaatcg agccgaagta
      721 tggtgcacac ccgcagcggt accggaactc ttgctgccac acagctagac cgccgagcgc
      781 gtgagtggaa atgttctcgc aggtcacgcg ctatcgacgc tggcagtccc taacctgaga
      841 acgcgggaac cttaccgctg ttcgtcgttc cctgatgctt cgcttcccac gcttggacga
      901 cgttgagcgg cagccggcca cggtcactga cattgtgacc attctgcttg gcccactcgc
      961 ggatggcctt gagcgtgtcg cggttgtagc cgcttcttgc gacgctcccg ttgccagccg
     1021 tacggccacc cgaacggtca gcgccacgag tgcctgggcg ccgcccactg gtcttacggg
     1081 cagctgcgac gtaacgagcc aagttatcac ggagcttggc tgcgttgcca tccgccaggt
     1141 cgatctcgta cgtgatgccc tcgagtgcga actcgactgt cgtaatgtcc tcagcttcct
     1201 ccccggtgag gtcgtcgatg agggtgacga tggtcttctg agccacgggt acggattctc
     1261 cttgtcgaca ggaacacggc gtggacgaaa ctgccgcgtt gtgaacgctg atcgcgacaa
     1321 gatacacaca atgccggtga tgcgaacacg cggcatgccg taacggcccg tttccccacg
     1381 atatgcctcc cgaactcaac aactacagct gttggagcag cgcggcacag ctgcgctgtc
     1441 gagttcggga agcgcagagc ggtcgcgagg gcgcgccgcg tcgacgcaga tgatcggtgg
     1501 tcagccggcg cggcgtaggc gcgcgacctc cgcgagcacg acaccagcag cgacggacgc
     1561 gttgagcgac tcgaccggcc ccgctatcgg tatcgacact gaagcgtcgc atgtctcgcg
     1621 gaccaggcgg gacaatcccc gcccctccga ccccagaacc agcgccaagg gtccggtggc
     1681 ccacggaaac gagtccagtg acatcgagcc accggcgtcc agcccgacca ccatcaggcc
     1741 ggatgcggcg cagtccttga gcgtacgcac cagattggtg gcccgggcca ccggtagacg
     1801 cgcagcggtt cctgccgaag tccgccacgc caccgcggtc atccccgccg agcgccgctg
     1861 cggcaccag
//
LOCUS       DATADN010001157         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_599330, whole genome shotgun sequence.
ACCESSION   DATADN010001157 DATADN010000000
VERSION     DATADN010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599330"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VHH53_14835"
     CDS             <1..627
                     /locus_tag="VHH53_14835"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2291427.1"
                     /translation="DLAEERTRTVNRLHALLRDLIAGGAKRNLTATHAAGLLRTVRPM
                     TPADEHRKSLAQDLLADLRRLDRALLSNAEQIQAAVNASKTTLTTLHGIGPVLAAKIL
                     GHTSEISRFPTRHHYASYCGTAPIEACSGEQRRHRLSRAGNRQLNRALHFVAVCQIRT
                     SSPGKHYYQQKLVEMKTPAEARRALKRRLSDAVYRRITKDQQDSPADT"
     gene            complement(650..>1746)
                     /locus_tag="VHH53_14840"
     CDS             complement(650..>1746)
                     /locus_tag="VHH53_14840"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2291428.1"
                     /translation="CDRICALDSDEAAVGTLTLGPIGQPHLPSRRSRLASMIDPRQIR
                     ARVFAQSGDPSAAEVTMTDTRVNDHPAVLVCVPAFRRTLDQQIVPRLLVRLVDSLSAD
                     PRGTAVLTASPTPTGRRRERYFEGIVPLRGSSVENLRADVFDALSDVPPARDEAINQL
                     RALLLTGPAELREQLRGLTTGKLINTCAGLRPTTGLADPVQATKAALRRLARRHQQLS
                     AEIRELDAELAPLVTATVPELLALPGVGIEVAGQLLVTAGDNPERLRSEAAFAHLCGV
                     APIPASSGRTDRHRLNRGGDRAANTALHTIVLSRLRWDPRTRAYLARRRHQGLTKPEA
                     MRCLKRYIAREAYRKLINTPYHTKDKPAAA"
BASE COUNT          291 a          555 c          609 g          291 t
ORIGIN      
        1 gatctcgctg aggagcggac ccggaccgtt aaccggttgc acgcgctgtt gcgtgacctc
       61 atcgcgggcg gtgcgaaacg gaacctgacc gccacccacg cggctggctt gctgcgcacg
      121 gtgcgcccga tgaccccggc cgatgaacac cgcaagtcac tggcgcagga tctcctggct
      181 gatctgcgtc gactcgatcg agcgctactt agcaatgctg aacagatcca ggccgcggtg
      241 aacgccagca aaacgacgtt gaccaccctg cacgggatcg ggccggtgct cgcggccaag
      301 attttgggtc acaccagtga gatcagtcgg ttcccgaccc ggcatcacta cgccagctac
      361 tgcgggactg cgcccatcga ggcctgcagc ggtgagcagc gccgacaccg gctctcgcgt
      421 gccggtaacc gacaactcaa ccgggcgctg cacttcgtgg ccgtctgcca aatccgtacc
      481 tctagtcccg gcaagcacta ctaccagcag aaactcgttg agatgaagac tcccgccgaa
      541 gcgcgacgcg cgctgaaacg ccgtctgagc gacgcggtct accgacgcat caccaaagat
      601 caacaagact caccggcgga cacttgacac acagaggcgc tatggttcgt caagccgctg
      661 cgggcttgtc tttggtgtgg tacggggtgt tgatgagttt tcggtaggcc tcgcgggcga
      721 tgtatcgctt gaggcatcgc atggcttcgg gtttggtgag tccttggtgt cgtcgccggg
      781 ccaggtaggc gcgggtacgg gggtcccagc gcagtcggga tagcacgatg gtgtgcagcg
      841 cggtgttggc cgctcggtct ccgccgcggt ttaaccggtg gcggtcggtg cgtccagagc
      901 tggcggggat gggtgcgact ccgcacaggt gggcgaaggc ggcttcggag cgcagccgct
      961 caggattgtc cccagcggtg accaggagtt gaccagcgac ctcaatgccc acaccgggca
     1021 gagccagtag ttcaggtacg gtggcggtga ccagcggcgc gagctcggca tcgagctcgc
     1081 ggatttctgc gctcagctgc tggtgacggc gggccagccg gcgcagcgcg gccttggtgg
     1141 cctgcaccgg atcagccaga ccggtcgtgg gacgcaaccc tgcgcaggtg ttgatgagct
     1201 tgccggttgt caggccacgc agctgctcac gcagttcagc cgggccggtg agcagcaagg
     1261 cgcgcagctg gttgatcgcc tcgtcccgcg cgggtgggac gtcgcttagc gcgtcgaaca
     1321 cgtccgcgcg caggttctcc acgcttgatc cgcgcagcgg aacgatcccc tcgaagtacc
     1381 gctcccgccg ccgtccagtc ggcgttgggg aggccgtcag aaccgccgtg ccccgcggat
     1441 cggcggacag cgagtccacc agccggacca gcagacgtgg cacgatctgc tggtcgaggg
     1501 tgcgtcggaa ggccgggacg cacacgagca cggcggggtg gtcgttcacg cgcgtgtcgg
     1561 tcatagtgac ttcggccgcc gaagggtcgc cggattgcgc gaacacccgg gcccggatct
     1621 gccgcggatc gatcatgctg gcgaggcggc tgcgccttga cgggagatgc ggttgtccga
     1681 tcggacccag ggttagcgtc ccgacggcgg cctcgtcgct gtccaaggcg cagatccggt
     1741 cgcaga
//
LOCUS       DATADN010001158         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1505836, whole genome shotgun sequence.
ACCESSION   DATADN010001158 DATADN010000000
VERSION     DATADN010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505836"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VHH53_14845"
     CDS             <1..300
                     /locus_tag="VHH53_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2291429.1"
                     /translation="AKGAVVALTRSMAIDLAPRGIRVNAICPGTVLTPLMEPMLRARG
                     NGDMEVGLARTVVKYPIGRLGTPEEIASAALFLASAGASFLTGSILAADGGMTAQ"
     gene            311..1231
                     /locus_tag="VHH53_14850"
     CDS             311..1231
                     /locus_tag="VHH53_14850"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX2291430.1"
                     /translation="MGFHLDPDKRVPLDRAAALVTDGAMVALGGGLSARLPMALVREL
                     IRQRRSGLHLVGSAHGIDVDLLVAVGAVGICEESYVGFEQDLGLAPAFRRAAENGTIE
                     VRESCCDTILTQLRAAEFGLPFLPVRGVKGSDIGRLHPEYGEVHCPFTGETLVAVPPL
                     RPDAVLVHAPLADRRGNLHLEQPYVLDERFAAASGTVVATTERIVSTDEVIAAGITIP
                     AHLVAAVSEVPFGAHPSSCYPAYAYDRPHLAGYVRAASAGSDELAGYLDRFVTGTPTE
                     EAYRKVVGEEQLAALGRWSQSVEAWKGLFG"
     gene            1228..>2067
                     /locus_tag="VHH53_14855"
     CDS             1228..>2067
                     /locus_tag="VHH53_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291431.1"
                     /translation="MTWSLDEWFVVALARTIRDREVTFHGFGSPCAQVAMHVAKRTHA
                     RGMMLVEGATYACNPDPPFIPPTGNEASLQRGAAYRMRFEEFFDAAVRGDVDRMFLSG
                     GQIDAYGNTNVTAIGAPDYPAASPKVKLGGGGGGCNLAATIRHLTLWTTRHRSGRALV
                     PRCDFITDMGHRTPAGSRAELGYSGGGPQWLVTELGIFDYDAQGRARLRQLFPDVGLD
                     DVRAATGFELQVAPDLGPVRPPSPAELAVLRGIDPLRVRRSEFSPVELHRTFSHSDNA
                     GCAC"
BASE COUNT          291 a          695 c          745 g          336 t
ORIGIN      
        1 gccaagggtg cggtggtggc gctgacccgc tcgatggcga tcgacctcgc accgagaggg
       61 atccgggtga acgcgatctg cccgggcacg gtgctcaccc cgctgatgga gccgatgctg
      121 cgggcccgtg gaaacgggga catggaggtc ggattggcca ggacggtggt caagtaccca
      181 atcggccggc tgggcactcc ggaggagatc gcctcggcgg cgctgttcct ggccagtgcc
      241 ggtgcctcgt ttttgaccgg gtcgatcctg gccgccgacg gcggcatgac ggcgcagtga
      301 ggggcgtgct gtgggctttc acctcgaccc cgacaagcgc gtcccgctgg atcgggccgc
      361 cgcactggtc accgacggcg ccatggtggc cctgggtggg gggctgtccg cccggctgcc
      421 gatggcgctg gtccgggaac tgatccggca gcgccgcagc gggctgcacc tggtcggctc
      481 ggcgcatggc atcgacgtgg acctgctcgt cgcagtcggc gcggtgggga tctgcgagga
      541 gagctacgtc ggtttcgagc aggacctcgg cctggcgccg gcgttccggc gggccgctga
      601 gaacgggacg atcgaggtcc gggagagctg ctgcgacacg atcctcacgc agctgcgtgc
      661 cgccgagttc gggctgccct tcctgccggt gcgaggtgtg aagggttctg acatcgggcg
      721 gctgcatccg gagtatggcg aggtgcattg cccgttcacg ggggagacgc tggtcgccgt
      781 gcccccgctt cgaccggacg ccgtcctggt gcacgctccg ctggccgacc gccgcggcaa
      841 cctgcacctg gagcagccct atgtactgga cgagcgcttc gcggccgctt ccgggacggt
      901 ggtggctacc acggaacgga tcgtcagcac cgacgaggtc attgcggccg ggatcaccat
      961 cccggcacat ctcgtcgcag cggtgagcga ggtgcccttc ggtgcccacc cgtcgtcctg
     1021 ctacccggct tatgcgtatg accggccgca tctggccggc tacgtccgcg ctgcctcggc
     1081 agggagtgac gagctggccg gctacctcga tcggttcgtc accggcacac ccactgaaga
     1141 ggcctaccgc aaggtcgtcg gcgaggagca actcgccgcg ctgggccgct ggtcacagtc
     1201 cgttgaggcg tggaagggac tgttcggatg acgtggtcgc tcgatgagtg gttcgtggtc
     1261 gcgctggccc gcaccatccg ggaccgggaa gtcaccttcc atggcttcgg cagcccctgc
     1321 gcgcaggtcg ccatgcacgt tgccaagcgc acccacgccc ggggcatgat gttggtggag
     1381 ggagcgacct acgcctgcaa tcccgatccg ccgttcattc cacccaccgg caacgaggcc
     1441 agcctgcaac gcggcgcagc ctaccggatg cggttcgaag aattcttcga cgccgccgtg
     1501 cggggcgacg tcgaccggat gttcctgtcc ggcggccaga tcgacgccta cggcaacacc
     1561 aacgtcaccg cgatcggcgc cccggactac ccggcggcat cgccgaaggt gaagctcggc
     1621 ggcggcggcg gtgggtgcaa cctggcggcc accatcaggc acctgacgct atggacgacg
     1681 cggcatcgtt ccggacgggc gttggtgccg cgctgcgact tcatcaccga catgggccac
     1741 cgcacgcccg cgggcagccg cgccgagctt ggctattccg gtggcggacc acaatggctg
     1801 gtcaccgagc tggggatctt cgattacgac gcccaagggc gcgcccggct gcgccagctg
     1861 ttccctgacg tcggcctgga cgatgttcgc gctgccaccg gtttcgagct gcaggtggct
     1921 cccgatctag ggccggtgcg cccgccgagt ccggcggagc tggccgtgtt gcgggggatc
     1981 gaccccctgc gggtgcggcg ctcggagttc tccccggtcg agctgcatcg cacgttcagc
     2041 cacagcgata acgctggatg cgcatgc
//
LOCUS       DATADN010001159         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_284131, whole genome shotgun sequence.
ACCESSION   DATADN010001159 DATADN010000000
VERSION     DATADN010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284131"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1135)
                     /locus_tag="VHH53_14860"
     CDS             complement(203..1135)
                     /locus_tag="VHH53_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023525214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEX2291432.1"
                     /translation="MAGKLASQVRGLSEAQFRETYGTEEQCRAVVEKLRWPSGFVCPV
                     CGGCEGKWLSTRPKIQCRACRHQVSLTAGTIFHSTKLPLTSWFLAMWLIATAKNGISS
                     VELGRRLGIKQTNAWSLKQKIMAVMAGREDKKRLEGRVEMDDVYLGGHRPGKRGRGAA
                     GKQPFVAAVSTSDERRPRKIKLLPVKGFRKKEIKKLVAKHLASTSRLITDGLRCWTAA
                     AEAGIGHTPIITGSGKRAAGWSPFKWVNTTLGNVKAAITGTYRSVSPDHAGRYLASFA
                     WRFNRRFQLTTLIPRLVHSAVRTTPLPYHQLVAS"
BASE COUNT          252 a          480 c          491 g          287 t
ORIGIN      
        1 ccagcgggtg ttggcgatgg cggtgccggc cagggtcagc aggggcatcg ccacgaagtc
       61 aaccggcgcg ctgagccacc cccacggaag tggtccatcc tgaggtatgt ctttggacag
      121 gatggaggac cgcgatgccg caagcaggct gcggaaatag tgcggctgtg agctgatttg
      181 gctttttgag tagcctgcaa agtcagctgg cgacgagctg gtggtagggc aacggagtgg
      241 ttctgacggc gctgtggacg aggcgcggga tcagggtggt gagctggaag cggcgattga
      301 agcgccaggc gaagctggcg agatagcggc cggcatggtc ggggctgacg ctgcgatagg
      361 tcccggtgat cgcggccttg acgttgccga gggtggtgtt gacccacttg aacggtgacc
      421 agcccgccgc ccgcttcccg ctgccggtga tgattggcgt gtgcccgatc ccggcctcgg
      481 ccgcagcggt ccagcagcga aggccgtcgg tgatgaggcg gctggtcgag gccaggtgct
      541 tggccacgag cttcttaatc tccttcttgc ggaagccctt gaccggcagg agcttgatct
      601 tgcgcggccg acgctcgtcg ctggtcgaga ccgccgccac gaagggctgc ttgccggcgg
      661 caccgcgccc gcgtttgccg ggcctgtgcc cgcccagata gacgtcatcc atctcgaccc
      721 gaccctccag gcgcttcttg tcctcgcggc cggccatcac cgccatgatc ttctgcttca
      781 ggctccaggc gttggtctgc ttgatcccga gccttcgccc gagctcgacc gagctgatcc
      841 cgttcttggc cgtcgcgatc agccacattg ccaagaacca gctggtcagc ggcagcttcg
      901 tgctgtggaa gatggtgccc gctgttagcg acacctgatg ccggcaggcc cggcactgga
      961 tcttgggccg ggtgctcagc cacttgccct cgcagccgcc gcagaccgga cagacgaacc
     1021 cggaaggcca gcgcagcttc tcgaccaccg cccgacactg ctcctcggtc ccgtaggtct
     1081 cacggaactg cgcctcgctc agtccgcgca cctgcgacgc cagctttccc gccatcccgc
     1141 ctctccgccc aaagccaact ctctgagaga gatagcggag agtcgctgcc tttttaggct
     1201 attcaggaac tcgattgtgt ggggggaatt cggctggctc gtcttccgcg gctgttcgcg
     1261 cttcgagtgg tcgatcatct cggggctcca ctcacagacg acggtagatc caccaccgtc
     1321 aggagaacag atggcccgtg tgcaggccat agcaccgggc taaagcccca tgggctggct
     1381 tttctttcgt gatggaagtt caccgcctgc ctaccagccc gttgacaaag tcgtggctcg
     1441 acaaccagca taccagatct gcaaatcatg atggagttcg accacaacat gttgggtacg
     1501 gccaagcggc
//
LOCUS       DATADN010001160         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_127383, whole genome shotgun sequence.
ACCESSION   DATADN010001160 DATADN010000000
VERSION     DATADN010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127383"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..692)
                     /locus_tag="VHH53_14865"
     CDS             complement(246..692)
                     /locus_tag="VHH53_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2291433.1"
                     /translation="MSVEVQAVDQVTDELVDAAGRLLRQLSSSAAPLDTNDLTRILEH
                     QATTLFVARSQSVIVGMLTLVTFPLPSGLRARIEDVVVDQDARGQGVGTALTMAAIDQ
                     AQAQGARSIDLTSRASRVAANRLYQQLGFQLRDSNVYRYQPQPRPH"
     gene            691..1071
                     /locus_tag="VHH53_14870"
     CDS             691..1071
                     /locus_tag="VHH53_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291434.1"
                     /translation="MARTVPDRAVSSAYAGVVLGLGRLLPQNSSLVVAAATLAVAAAF
                     QPARRRIQAAVDRRFNRGRYDAARTIAAFSTRLRDQVDLSTLTSELLTVVDQTMQPTR
                     ASLWLRPQGPSNAIVAGVDTPRPS"
     gene            complement(1275..>2114)
                     /locus_tag="VHH53_14875"
     CDS             complement(1275..>2114)
                     /locus_tag="VHH53_14875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2291435.1"
                     /translation="VERRYGRSILNSLHGLWSVGAVLGGAMGAAAAGLGLALVWHFAV
                     AAGLFAALAVGASRFLLPGLDNTERPASADGTPGRGLRLGRPGVARRVLALGVIAALA
                     QVMEDTGGAWGVVYLREDLDAAAAVSGLGFVALQTCQTFGRLLGDRAVSRYGDRAVAR
                     AGTAVAGGAITAALALPATASTVLALGAIGLGIGTLIPASMRTADNLPGLPRGLGLTM
                     AGTVNRIAMLAAPPLIGVIADSYGLRVGLLVIPLAAALVLLLASALPASPSAPSDSLS
                     DAP"
BASE COUNT          344 a          732 c          735 g          303 t
ORIGIN      
        1 tcttgaacat cacagagagt gccctgctgc tgctgatcgt ctggtacgcg tggacgtggc
       61 ccaggcggga gcgaacaccc tgaggggttc gtaccctgtc cattactgct gcgccgtccg
      121 aggtcgtagc ggtggaccgc ctgtgacctg caatccgcat tggccgagca tgaccgctcg
      181 tgcccgccgg gaaccaggag gtcctcatgc cgtgcggacc cagcacggac cagcgctgat
      241 gaagctcagt ggggccgagg ctggggctgg tatcggtaga cgttggagtc gcgaagctgg
      301 aagccgagtt gctggtagag ccggttggcc gcgacccggg aggcccgaga ggtcagatcg
      361 atgctgcgag ccccctgcgc ttgcgcctgg tcgattgccg ccatggtcag cgcggtgccg
      421 acgccttggc cgcgggcgtc ctggtcgact acgacatctt cgatccgtgc ccgcagccca
      481 gaggggagcg ggaaggtgac cagagtcagc atgccgacga tgacgctctg gcttcgggcg
      541 acgaacaagg tggtggcctg atgttcgagg atgcgggtca gatcattggt gtccagtggt
      601 gctgcggagc tagagagctg gcgcaggagg cgtccggcgg cgtcgacgag ctcgtcggtg
      661 acctggtcga ccgcttggac ctccacgctc atggcgcgga ctgtacctga ccgggctgtc
      721 agttccgcct acgccggagt ggtgttggga cttggccggc tgctgcccca gaactccagc
      781 ctggtcgtgg ccgccgccac cctggccgtg gccgccgcat tccagccggc ccgccgccgc
      841 atccaggctg cggtcgaccg ccgcttcaac cggggccgct acgacgccgc ccgcaccatc
      901 gccgcgttca gcacccggct gcgggaccag gttgatctga gcacgctgac cagcgagctg
      961 ctgacggtgg tcgaccagac catgcagccg acccgggcgt cgctgtggct gcgtccacag
     1021 ggaccgtcca acgccatcgt cgctggggtg gacacaccaa ggccgtccta gcatcaacca
     1081 gcgaccgtcg gatgtccctc ccgctgcttg gaactggagg tcggatcgac cgacgacctt
     1141 ctgattacga gacggatgct cggcgtcgga ccagatggct ccagacggat ctggcctgct
     1201 cacgttggat gcctcgtcgg tccagaccgc tccagacggc tccagacggc tcgtctggat
     1261 gatcatcgag atgatcaagg cgcatccgac agagaatcgg atggcgctga cggtgaggct
     1321 ggcagggccg aggcgagcag caggacgagc gccgccgcca gcgggatcac cagcagcccg
     1381 acgcgcaggc cgtagctgtc ggcgatgacc ccgatcagcg gcggggcggc cagcatggcg
     1441 atccggttca ccgtcccggc catggttagg ccgaggccgc gcgggaggcc gggcaggttg
     1501 tcggcggtgc gcatgctggc cgggatcaag gtgccgatcc ccaacccgat agctcccaaa
     1561 gccagcaccg ttgaggcggt ggccggtaag gccagggcgg cggttatcgc cccgccggct
     1621 accgcggttc ccgcccgggc gacggcgcgg tcgccgtagc ggctgacagc acggtccccc
     1681 agcagccgac caaaggtctg gcaggtctgc aaggccacga agcccaggcc gctgaccgca
     1741 gcggcggcgt ccaggtcctc gcgcaggtac accacgcccc aggccccgcc ggtatcctcc
     1801 atgacctgag ccagagcggc gatcaccccg agggccagca cgcgccgggc gacgcccgga
     1861 cgcccaagcc ggaggccgcg acccggcgtc ccgtcggcgc tggccggccg ctcggtgttg
     1921 tccagcccgg gcaacagaaa ccgggacgcc cccaccgcga gggctgcgaa cagccctgcc
     1981 gcgactgcga aatgccacac cagcgctagt cccaggccag ccgccgcggc acccatcgca
     2041 ccgccgagga ccgcccccac gctccacagg ccatgcaggg agttcaggat cgaccggcca
     2101 tagcgacgct ccac
//
LOCUS       DATADN010001161         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_599813, whole genome shotgun sequence.
ACCESSION   DATADN010001161 DATADN010000000
VERSION     DATADN010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599813"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..826)
                     /locus_tag="VHH53_14880"
     CDS             complement(29..826)
                     /locus_tag="VHH53_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEX2291436.1"
                     /translation="MLSYPSGMTVSSRALGVLSDALRAQRNQRRTRWRKLSPGRQALL
                     VVAHLRKGETYADLACGFRVGTSTVYRYIREALELLAAMAPTLAQAIEVARRKAFVIL
                     DGTLLRIDRVGMTSGYDRAFYSGKHKAHGLNVQVVADPIGRLVWISPPLPGARHDMGA
                     AREHGIIDALATHEIPAAADTAYQGAGPTVAVPQRRRRKDLGTGRYRRLSRNQKDVNT
                     AHARRRGPGERANAELKNWRVLRKIRSSPNTAHRLIAAVQTLMIASA"
     gene            complement(943..1608)
                     /locus_tag="VHH53_14885"
     CDS             complement(943..1608)
                     /locus_tag="VHH53_14885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2291437.1"
                     /translation="MSENEALDRTRRDYDEVAELYDDMVRQGDQVIDALSAGMINAFA
                     DLVRAGGSVGAVVDAGCGPGQWTDHLDRAGIRAYGIDLSPAMVAIARRYRPDLRYEVG
                     SMLHLDAGDESIAGILAHFSLIHTPPDLLPRVLTEFARVVEPGGPLLIGVQITDTADA
                     NGWVPYDHRASPAYLWTLDALADRLREHDFAELGRLRIVAPAPGKPPAGYLLARHDAR
                     SNE"
BASE COUNT          307 a          667 c          656 g          302 t
ORIGIN      
        1 ctgacgcact gagcctttgc caacctgatc atgcgctggc gatcatgagg gtctgaactg
       61 cggcgatgag ccggtgggcg gtgttcggac tggagcggat cttccgcagg acccgccagt
      121 tcttcagctc ggcgttggcc cgctcgccgg gtccacggcg gcgggcgtgg gcggtgttga
      181 cgtccttctg gttccgggac aggcgccggt agcggccggt gcccagatcc ttccgacggc
      241 gccgctgcgg aaccgcgacc gtcggcccgg cgccctgata ggcggtgtcg gccgccgcgg
      301 ggatctcgtg ggtggccagc gcgtcgatga tcccgtgctc acgggcagcg cccatgtcat
      361 gtctcgcgcc gggcagcggc ggcgagatcc acaccagccg gccgatcggg tcggcgacga
      421 cctgcacgtt cagcccgtgg gccttgtgct tgccggagta gaaggcccgg tcatagcccg
      481 atgtcatgcc tacccggtcg atgcgcagca gggtgccgtc gaggatcacg aacgccttgc
      541 ggcgggcgac ctcgatcgcc tgggccaggg tgggagccat cgcggcgagc agttcgagcg
      601 cctcacggat gtaccggtag accgtcgagg tcccgacgcg gaagccgcag gccaggtcgg
      661 cgtaggtctc gcccttacgc aggtgcgcga ccacgagcag cgcctgccgg ccgggcgaga
      721 gcttccgcca ccgggttcgg cgctggttgc gctgcgctcg cagcgcatcg gagagcacgc
      781 cgagggcgcg gctggacacg gtcatcccgg acgggtagga cagcacgttg aagctcctgg
      841 tgggacgtgt tgatctaggc aatcgcccgt ctaccaggag attcacccat tcgaggtcac
      901 gccacgttgc acaccgctga ctgcccgccc aggtcggcaa attcattcgt tacttctggc
      961 gtcatggcgc gccagcaggt atccggcggg gggcttgcct ggcgccggag caacgattcg
     1021 caggcgtccg agctcggcga agtcgtgctc gcgcagtcgg tcggcgagcg cgtcgagggt
     1081 ccacagatac gccggtgagg ctctgtggtc gtacgggacc caaccgttgg cgtctgcggt
     1141 gtcggtgatc tgcacgccga tcagcagggg tccgcccggc tctaccactc gggcgaactc
     1201 ggtcaggacg cgcggaagca ggtccggcgg cgtgtggatc aacgagaagt gcgccaggat
     1261 gcccgcgatc gactcgtccc ctgcgtcgag atggagcata gaaccgacct cgtagcgcag
     1321 gtcgggacga taccggcggg cgatcgcgac catggccggt gacagatcga tcccgtaagc
     1381 ccggataccg gctcgatcca ggtgatcggt ccactgtccg ggaccgcaac ccgcatcgac
     1441 cacagcaccc accgacccac cggcgcggac gaggtcggcg aaggcgttga tcatccctgc
     1501 tgacagcgca tcgatgactt ggtcgccctg tcgcaccatg tcgtcgtaca actcggcgac
     1561 ctcgtcgtag tctcggcggg tccgatccaa cgcttcgttc tcactcaccc gccggactct
     1621 agggaacagg tcggacgtag gagtgtcgga cactccaacc gcgaaggcga cgcgatcgat
     1681 ccgtccgccc aggacggtgg gcaccacctt taccaccgcc cccaccaagc tgtccacgac
     1741 ggccgcagcg ctggcacatc cactcccggg ccgcacgcca cgccgcacaa ccgctggcca
     1801 gccccctcac caggttggca aaggctcact ggctcccccg caacgaccac tcgacgaggc
     1861 cgggcaggct ctccattcgg gccgtgcagg acgcacagct ccgccccggc cccttctaca
     1921 cctggaccgc gg
//
LOCUS       DATADN010001162         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_885776, whole genome shotgun sequence.
ACCESSION   DATADN010001162 DATADN010000000
VERSION     DATADN010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885776"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            508..>2388
                     /locus_tag="VHH53_14890"
     CDS             508..>2388
                     /locus_tag="VHH53_14890"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2291438.1"
                     /translation="MSTTGTFRRIKKTLPRYDYEHYSRLAGPLTQPDPTKPYKVQYRS
                     LLSQEPHRIRVALMLAAAPVLSVVLLVWLLQPEHWTERDYPAFDWLPALDVVMLVSIG
                     LIEFFRCMNVLSNAHATLVARDPIPVVPETGTRVAFLTSFVPGKEPLEMVTKTLEAAV
                     KIRHRGLMHVWLLDEGDDPEVKAVCERLGVHHFSRKGVAKWNQAKGPHRAKTKHGNYN
                     AWLEAHGDDYDFFASVDTDHVPLPNYLERMLGYFRDPDVGFVIGPQVYGNYDSFVTKA
                     AESQQFLFHALIQRAGNRYGSPMFVGTSNAVRIKALKQIGGLYDSITEDMATGFEMHR
                     HKNPATGRKWRSVYTPDVLAVGEGPSAWTDFFTQQMRWSRGTYETIIGQYWKGWYSLP
                     PSKLFNYTMMIIFYPMSALNWILAALSCCLFLGLGASGVNIDPAVWLMLYGNASALQI
                     GLYVWNRRHNVSPHEPEGSGGVAGMVMSALSAPLYAKALIDSVLRRKSKFVVTPKGDS
                     ASPDRWFGTFRYHWYFVAIFGASIAAGFVFGHSHPAMIIWATFALLITASPVFAWRWQ
                     LRQDAKKPAAPAVEQPQDPAGPNRTQPLPIPQQPAAHAPQHKPGWAAAPGGNGEVGGT
                     DQT"
BASE COUNT          414 a          816 c          782 g          376 t
ORIGIN      
        1 cgggcggagg aggccataag agagcacttc gggaacatcc gcgcccgcct gtccacaacc
       61 actccacagg ctccccacac ccaccacaat gaacgcgtat gaccggtaaa cgccgtgttt
      121 cgcaccttgc gatcatgatg aacaccgtag aaccctggtt ttctggtttt tcaagatgct
      181 gcgcatacgc ggcagttggg ggcaaaggaa ccggttgcgg gccgggcggg ggtaggggcc
      241 cccgttcatg aagcgatgcc gtggggggca tcatgcaacc ggaaggtcgc gttgccgtgt
      301 ctataaggtg tggatgacgt ggtggcagcg acccaccact gatacgcctg tgaaggtcga
      361 gggggacctg tagcaggcgg taggaagcta tgaaacacgc ggtgtccgcg tgtgggggga
      421 tgactcatga cgtcgaggcc gacgggcgcc cggcagcagg accacgaccc gtcccagacc
      481 acccagctca gggtgccgac gcatcggatg agcaccacgg ggacgttccg gcggatcaag
      541 aagacgctgc cgagatacga ctacgagcac tacagccggc tcgcgggtcc cctcacccag
      601 cccgatccga ccaagcccta caaggtgcag taccggtcgc tgctctcgca ggaaccgcac
      661 cgcataagag tcgcgctgat gctggccgcg gccccggtgc tgtccgtggt gctgctggtg
      721 tggctgctcc agcccgagca ctggaccgag cgcgactacc cggccttcga ctggctgccc
      781 gcgctggacg tggtgatgct ggtctcgatc ggcctgatcg agttcttccg ctgcatgaac
      841 gtgctgtcga acgcgcacgc cacgctggtc gcccgcgacc cgatcccggt ggtgcccgag
      901 accggcacca gagtggcatt cctcacctcc ttcgtgcccg gcaaggagcc gctggagatg
      961 gtgacgaaga cgctggaggc cgcggtgaag atccgccacc ggggcctcat gcacgtctgg
     1021 ctgctcgacg agggcgacga ccccgaggtg aaggcggtct gcgagcgtct cggcgtgcac
     1081 cacttctccc gcaagggcgt cgccaagtgg aaccaggcca agggcccgca ccgcgccaag
     1141 accaagcacg gcaactacaa cgcctggctg gaggcgcacg gcgacgacta cgacttcttc
     1201 gcctcggtcg acaccgacca cgtgccgctg cccaactacc tggagcggat gctgggttac
     1261 ttccgcgacc cggacgtcgg cttcgtcatc ggcccacagg tgtacggcaa ctacgactcg
     1321 ttcgtcacca aggccgccga atcgcagcag ttcctcttcc acgcactgat ccagcgcgcg
     1381 ggcaaccgct acggctcccc catgttcgtc ggcacctcca acgccgtgcg catcaaggcg
     1441 ctgaagcaga tcggcggtct gtacgactcg atcaccgagg acatggcgac cggcttcgag
     1501 atgcaccgcc acaagaaccc ggcgacgggc aggaagtggc gctcggtcta caccccggac
     1561 gtgctcgcgg tcggtgaggg ccccagcgcc tggacggact tcttcaccca gcagatgcgc
     1621 tggtcgcgag ggacgtacga gacgatcatc gggcagtact ggaagggctg gtactcgctg
     1681 ccgccgagca agctcttcaa ctacacgatg atgatcatct tctacccgat gtcggccctg
     1741 aactggatcc tggcggcgct gagctgctgc ctgttcctgg gcctgggcgc ctcgggtgtg
     1801 aacatcgacc cggcggtctg gctgatgctc tacggcaacg cctccgccct gcagatcggc
     1861 ctctacgtct ggaaccgccg gcacaacgtc tcgccgcacg agccggaggg ttccggcggt
     1921 gtggccggca tggtgatgtc cgcgctgtcg gcgccgctgt acgcgaaggc gctgatcgac
     1981 tccgtgctgc ggcgcaagag caagttcgtg gtcacgccca agggtgactc ggcgagcccg
     2041 gaccgctggt tcggcacgtt ccgctaccac tggtacttcg tcgcgatctt cggcgcgtcg
     2101 atcgcggccg gcttcgtctt cggccactcg caccccgcga tgatcatctg ggcgacgttc
     2161 gcgctgctga tcaccgcctc gccggtcttc gcctggcgct ggcagctgcg gcaggacgcg
     2221 aagaagcccg ccgcccccgc cgtggagcag ccgcaggacc ctgcggggcc gaaccggacg
     2281 cagccgctgc ccatcccgca gcagcccgcg gcccacgccc cgcagcacaa gcccggttgg
     2341 gccgccgccc ctggcggaaa cggggaggtc ggggggaccg accagacc
//
LOCUS       DATADN010001163         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_746023, whole genome shotgun sequence.
ACCESSION   DATADN010001163 DATADN010000000
VERSION     DATADN010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746023"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..1037
                     /locus_tag="VHH53_14895"
     CDS             372..1037
                     /locus_tag="VHH53_14895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HEX2291439.1"
                     /translation="MWLLRPPGVYRPQADTWLLAQALHDATIPYAARVLDLCTGTGAL
                     AVAAARAGAAHVTAVDISRRAAMAARFNTMIRGLPVRVVRGNMLKFLAGRQFDVILAN
                     PPYVPCAHPDVPRRGPSRAWDAALDGRALLDPLCTNAAELLSVGGMLLIVHSAVSGVE
                     TTLDVLRQRGLKAAVVARRMEPFGPVMRSRIELLERQGLIEVGQRYEELVVIRGDRPG
                     PQP"
     gene            1063..1287
                     /locus_tag="VHH53_14900"
     CDS             1063..1287
                     /locus_tag="VHH53_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX2291440.1"
                     /translation="MRRDRRRVTVVAGGPVLIEGPVELVTDDGQVVTSDRFVVAVCAC
                     RRSRRYPFCDASHRRRVREDAEDSAGSNLD"
     gene            complement(1273..>1944)
                     /locus_tag="VHH53_14905"
     CDS             complement(1273..>1944)
                     /locus_tag="VHH53_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX2291441.1"
                     /translation="GNGVSHYLRDVAQWWQMREYIVHRSVYHLKEADPHAWVIPRLQG
                     QAKASLVAVEFDEFGGGRGERLHAQLYADLMAAAGLSPAYLHYLDDVPGPAITIVNMM
                     SMFGLHRRLRGAMVGHFAVAEITSAPSARRVAQALQRLGAPPACTHFFTEHVEADAVH
                     EQVLRRDVIGDLLRREPHLAADVVFGIAATELLEARLAGHLLTAWRNGTTSLHRPLPE
                     HSVQV"
BASE COUNT          331 a          594 c          649 g          370 t
ORIGIN      
        1 gagctggctg ttgtccggga acgggaacca cgacgcgaag aacgccgggt tcacccacgg
       61 atcgcacagc acactaccgg cgtgcgtttg gacgaacagc ccggcgtgcc cgagcccggt
      121 gacgcgcatc ctcatctcct ccgtgtcgtg ctgggtgccg tggctgcaag cgatggcagc
      181 ctatccgggg gctgaagttc accgtccaca gttggtggtc gacgtcaccg cgcggagttt
      241 tgggctctgc gtgcaggcat cgacggatgt ggcagtacgg gattttctgt ttgcccgctt
      301 caactagtcg ccgctggtgg ggaccggaac ggtccgtttg atcagatgtg aactggggta
      361 cttactacgc tgtgtggctg ctcagaccgc ctggcgtgta ccggccgcag gcagatacgt
      421 ggttattggc gcaggctttg cacgacgcga cgattcccta tgcggcacgt gttctcgatc
      481 tgtgtaccgg gaccggcgcg ctggcggtcg cggcggcccg cgccggcgca gcgcacgtca
      541 cggctgtgga catctcgcgc cgagccgcga tggcggctcg gttcaacacc atgatccgtg
      601 ggcttccggt ccgagttgta cgtggaaata tgctgaagtt cctcgccggt cggcagtttg
      661 acgtcattct tgctaaccca ccctacgtgc catgtgcaca cccggatgtt ccccgccgtg
      721 gaccgagtag ggcatgggac gcggctttgg atggccgggc gcttctcgat ccgttgtgca
      781 ccaatgctgc tgaactactc tctgtcggcg gaatgctgtt gatcgttcat tccgcggtat
      841 ctggagtcga gaccacgttg gacgtgttgc gccagcgagg actgaaggct gccgtcgtgg
      901 ctcgtcgtat ggagcctttc ggtccggtga tgcgaagccg gattgagcta ctggagcggc
      961 aaggcctgat cgaggtagga cagcggtacg aggaattggt ggtgatccgt ggtgaccgac
     1021 ccggtcccca accgtgatcc ggaaaactcg gttactgaac cgttgcgtcg agaccgaagg
     1081 cgggtcaccg tggtcgccgg cgggccggta ctgatcgaag gaccggtcga gttggtgacc
     1141 gatgacggcc aggtcgtcac ctcggaccgg ttcgtggtcg cagtatgtgc atgccggcgt
     1201 agccggcgtt acccgttctg tgacgccagc caccgccgac gcgtccgcga ggacgccgag
     1261 gactctgctg gatcaaacct ggactgagtg ctccggcagc ggacggtgca gcgacgtcgt
     1321 gccattgcgc caggcagtga gcaggtgtcc ggccaggcgg gcttccagca gctcggtcgc
     1381 cgcaatcccg aacacgacgt ccgcggcgag gtgaggttcc cggcgaagta ggtcaccgat
     1441 cacatcccga cgcaacacct gttcatgcac ggcgtcggct tcgacgtgct cggtgaagaa
     1501 atgcgtacat gctggtggcg cgcccaaccg ttgcagtgcc tgggccacgc gtcgcgcgct
     1561 gggcgcactg gtgatttcgg caaccgcgaa gtgtccgacc atggcaccgc gcagcctgcg
     1621 atgcagcccg aacatcgaca tcatgttcac aatcgtgatt gccggcccgg gcacatcgtc
     1681 caggtaatgc aaatacgctg gggacaaccc ggcagcggcc atgagatcgg catacagctg
     1741 agcgtgcagg cgctcgccac gaccgccgcc gaactcgtcg aactcgactg ccaccagtga
     1801 tgccttggcc tgcccctgca ggcgaggaat gacccaggcg tgcggatcgg cttccttcag
     1861 gtgatacacc gagcggtgca cgatgtactc gcgcatctgc caccactggg caacgtcgcg
     1921 aaggtaatgc gagaccccgt tgcc
//
LOCUS       DATADN010001164         3903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_285084, whole genome shotgun sequence.
ACCESSION   DATADN010001164 DATADN010000000
VERSION     DATADN010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285084"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /gene="thiG"
                     /locus_tag="VHH53_14910"
     CDS             <1..490
                     /gene="thiG"
                     /locus_tag="VHH53_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097900.1"
                     /note="functions in thiamine (vitamin B1) biosynthesis; in
                     Bacillus subtilis this enzyme catalyzes the formation of
                     thiazole from dehydroxyglycine and
                     1-deoxy-D-xylulose-5-phosphate and ThiS-thiocarboxylate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX2291442.1"
                     /translation="ALQTSWVKLEVIADDRTLLPDPIELLDAAEQLVNDGFTVLPYTN
                     DDPVLAGKLADVGCAAVMPLGSPIGTGLGIRNPHNIEMIVAQAQVPVVLDAGIGTASE
                     AAQAMELGCDAVLLATAVTRADDPPRMAEAMRLAVQAGRHARLAGRIPQRFWAQASSP
                     AP"
     gene            complement(555..755)
                     /locus_tag="VHH53_14915"
     CDS             complement(555..755)
                     /locus_tag="VHH53_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HEX2291443.1"
                     /translation="MADASMSSPEKNPEDWTTGDEPITGPQRSYLETLAREAGEDVPA
                     ELTKAQASELIDQLQEKTGRGR"
     gene            complement(796..3615)
                     /gene="ppc"
                     /locus_tag="VHH53_14920"
     CDS             complement(796..3615)
                     /gene="ppc"
                     /locus_tag="VHH53_14920"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX2291444.1"
                     /translation="MTSTARQESSAERGRRLDSVAAHASVRSESQHLALRADIRLLST
                     LLGETLVRQGGEELLKLVEEVRRLARATLADGSAEIRSLLSTLDIGTAVELTRAFSIY
                     FQLANVAEQLHRSREQRAQVGWRHGPLRAVMNRLARDVDRADVEAVLARMELRPVFTA
                     HPTEASRTSVQGILRRVADVLDQNLPKTQSQRRLARLVDLLWQTDEIRPGQPTVLDEA
                     RAVAYYLEQIGTHAVPDLLEDLEDELARVAFTLPAESRPLILGCWVGGDRDGNPNVSP
                     QVTLDVLRLYADRALNIHLTLVAQLVQELSVSTRVVGVSEALRCSLAHDRRVMPEVYD
                     RYLVRNADEPYRLKCSFVHARLTQTRQRIRDGMVHQEGRDYLGAQSYLADLEVMDTSL
                     RDHLGERIAEGTLARTLRSARALGLHLAALDVREHASKHHVALAALYDLLGELTTPYA
                     ELDREARTVLLSGELAGRRPLTARSAELPPAAADVLAVFDAIRSAQQTFGEAAASTYI
                     VSMTKGVDDVLAAAVLAREAGLMALAPPETTPADPAGTSRCTIDVVPLFETATELRCA
                     GELLDAMLRDRTYRQLVRARGDLQEVMLGYSDSNKDAGITTSHWDIHRAQRQLRDTAA
                     AHGVRLRLFHGRGGSVGRGGGPAGEAVAASPYGTVDATLKVTEQGEVISDKYSISALA
                     HDHLEILLAAVLEATLLHQTSRVPLEVLACWDEAMDVVSGAACAAYRNFVGLPGLGEF
                     FSAATPVDELGWLNVGSRPVRRPGSSKTTLEGLRAIPWVFGWTQTRMVVPGWYGVGSG
                     LRAARDAGLGPVLEEMRDWAFFSNLLGNVEMTLAKTDLRIAECYVSVLVEPSLQPLFD
                     AIVGEYELTKREVLRLTGGSTLLARHRLLRHTLQVRAAYLEPLHHLQISLLARHRAME
                     EPDLDLRRALLRTVNGIAAGMRNTG"
BASE COUNT          641 a         1340 c         1298 g          624 t
ORIGIN      
        1 ggcgctgcag acgtcctggg tcaagctcga ggtcatcgcc gatgaccgca ccctgttacc
       61 cgacccgatc gagttgctcg acgcggccga gcagctcgtc aatgacgggt tcaccgtgct
      121 gccctacacc aatgacgacc cggtgctggc cggcaagctt gccgatgtgg gatgcgcggc
      181 ggtgatgccg ctgggctcgc cgatcggtac cgggctgggc attcgcaacc cgcacaacat
      241 cgagatgatc gtggcgcagg cccaggtgcc ggtcgtcctc gacgccggaa tcggcaccgc
      301 ctcagaagcg gcccaggcga tggagctggg ctgcgatgcg gtgctgctgg ccactgccgt
      361 gacccgggcc gacgatccac cgcggatggc cgaagcgatg cggctggcgg tgcaagccgg
      421 ccggcacgca cggctggcgg gccgaatccc gcagcggttc tgggcccaag cctccagccc
      481 agccccttag ccccgcgtgt ccggctctcc tgcccggtgt gtcgaccgat ggtgcccggt
      541 gtgtcgaggg atctttagcg cccgcggccg gtcttttctt gcaactggtc gatcagctcg
      601 gacgcttggg ccttggtcag ctctgccgga acgtcctcac cggcctcccg ggcgagcgtc
      661 tcgaggtagc tgcgctgcgg cccggtgatc ggctcgtcgc ccgtggtcca gtcttcgggg
      721 ttcttctcgg gtgagctcat cgatgcatca gccatgctcg cgccataccc cgtgagttcc
      781 tgccgaaacc caggttcagc ccgtgttgcg catcccggca gcgatcccat tgaccgttcg
      841 cagcagggca cgacgcagat cgaggtcggg ttcctccatc gcgcgatggc gggccagcag
      901 cgagatctgg agatggtgca gcggctccag gtatgcggcc cgcacctgca aggtgtggcg
      961 caacaggcgg tggcgggcca gcagggtgga gccaccggtg agccgtagca cttcccgctt
     1021 ggtcagctcg tactcaccga cgatggcgtc gaacaatggc tgcaaactgg gctcgacgag
     1081 aaccgagacg tagcattccg cgatccgcag gtcggtcttg gccagcgtca tctcgacgtt
     1141 cccgagcagg ttgctgaaaa acgcccagtc ccgcatctcc tcaagcaccg gaccgagtcc
     1201 ggcgtcacgg gcggcccgca ggccgctgcc cacgccgtac cagcccggca ccaccatccg
     1261 ggtctgagtc catccgaaca cccaagggat ggcccgcagc ccttccagcg tggtcttgct
     1321 actccctggc cgccgcaccg gtcgggaccc gacattgagc cagcccagct cgtcgactgg
     1381 ggtagcggcg gagaagaact cgccgagtcc gggaaggccg acaaagttgc gataggccgc
     1441 acaggcggcg ccgctcacca cgtccatcgc ctcatcccaa caggccagca cctccagggg
     1501 cacccgcgag gtctggtgca gcagggtggc ctccagcacc gcagcaagca ggatctcgag
     1561 gtggtcgtgg gccagcgcgc tgatcgagta cttgtcggag atgacctcgc cctgttcggt
     1621 caccttcagc gtcgcgtcga ccgtgccgta gggcgaggcg gccaccgctt ctccagccgg
     1681 tccgccgccc cggccgaccg aaccaccgcg gccgtggaac agccgcagcc ggaccccgtg
     1741 ggcggcggcg gtgtcgcgta gctggcgctg cgcccggtgg atgtcccaat gcgacgtagt
     1801 gatcccggca tccttgttgg agtcggaata cccgagcatc acctcctgca gatccccccg
     1861 cgcgcggacc agctgccggt aggtgcgatc ccgcagcatc gcgtcgagca actcaccggc
     1921 gcagcgcagt tcggtggccg tctcgaacag cggcacgaca tcgatcgtgc acctgctcgt
     1981 accggccggg tccgcaggtg ttgtctcggg tggtgccagg gccatgagac cggcttcccg
     2041 ggccagcacc gcggcggcca gcacgtcgtc gacgcccttg gtcatcgaga cgatgtaggt
     2101 gctggcagct gcctcaccga aggtctgctg ggcagaccgg atcgcgtcaa agacggcgag
     2161 cacgtccgcg gctgctggtg gcagttcagc ggagcgggcg gtcagcggcc gccgtccggc
     2221 cagctcaccg gacagcagca ccgtccgcgc ctcgcgatcg agctcggcgt agggcgtggt
     2281 gagctcaccg agcagatcgt agagcgccgc gagggcgacg tgatgcttgc tggcgtgctc
     2341 gcgcacgtcg agagccgcca ggtgcaggcc taacgctcgg gcgctacgca gcgtcctggc
     2401 caacgtgccc tcggcgatgc gttcgccaag atggtcgcgc agcgaggtgt ccatcacctc
     2461 caggtcggca aggtaggact gcgcccccag gtaatcgcgt ccctcctggt gcaccatgcc
     2521 gtcccggatc cgctgccggg tttgggtcag gcgggcgtgg acgaaggaac atttcagccg
     2581 gtagggctcg tcggcattgc gcacaaggta gcggtcatag acctcgggca tcacgcgccg
     2641 gtcatgggcg aggctgcacc gtagcgcctc ggagacgccg accacccggg tggacacgct
     2701 cagctcctga acgagctgag caaccagcgt caggtgaatg ttcagcgcgc gatccgcgta
     2761 gagccgcaaa acgtcgaggg tgacctgcgg tgagacgttg gggttgccgt cgcggtcacc
     2821 gccgacccag caacccagta tcagcggccg tgactctgca ggcagggtga acgcgacgcg
     2881 ggcgagctcg tcctcgaggt cctccagcaa gtccggcacc gcgtgggtac cgatctgctc
     2941 gaggtagtaa gcgaccgcgc gagcctcgtc gagcaccgtc ggttggccgg gtcggatctc
     3001 gtctgtctgc cataacaggt caaccagccg cgcgagccgc cgttgcgact gtgtcttggg
     3061 caagttctga tcgaggacgt cggcgactcg gcgcaggatg ccctgcaccg aagtacggga
     3121 cgcctcggtc gggtgagcgg tgaacaccgg ccgcagctcc atccgcgcca gcaccgcctc
     3181 tacgtcagcc cggtcgacgt cgcgggccag ccggttcatt accgcccgca atggtccgtg
     3241 ccgccagccg acctgggcgc gctgctcccg cgagcggtgc agctgctccg ctacgttggc
     3301 gagctggaag tagatcgaaa atgcccgggt gagttcgacg gccgtgccga tgtccagcgt
     3361 ggacagtagc gacctgatct ccgcgctacc atctgccaga gtcgcccggg ctagccgtcg
     3421 tacctcttcc accaatttga gcagttcctc gccgccctgc cggaccaagg tctcaccgag
     3481 caaggtcgac agcaggcgga tgtcggcccg cagcgcgagg tgctgcgact ccgacctgac
     3541 actggcgtgg gcggccaccg aatccaggcg cctgcctcgt tcggcgctcg actcctgtcg
     3601 ggctgtcgac gtcatcggct ttccctcatc cagcgtcaat caagcgaagc taaagtggcg
     3661 ccaccaaagc gcgatagatt cagtccgccc gaatcctggc gatatccgaa gccggcgcat
     3721 ccatgcgatt aatagtcaca cgccgggtcg acaaatccca agcacaactc cgcaggcgtt
     3781 tcccactggt aacagcgcgg taatgctcag cgcagcgctt tcgggcctga tcgccgcctg
     3841 aggaacccgt aggcagcggt ggccacgaac agcaccgccc cggggacggt cagccagaac
     3901 aga
//
LOCUS       DATADN010001165         1594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_285164, whole genome shotgun sequence.
ACCESSION   DATADN010001165 DATADN010000000
VERSION     DATADN010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285164"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VHH53_14925"
     CDS             complement(<1..1183)
                     /locus_tag="VHH53_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX2291445.1"
                     /translation="MSAEATRAAAPTSVVEVHSIDYIPQSERHGKVTDQFTLWFLGNA
                     NLATLAVGLIAMGLGLPLVWVLVAIILGELFGTFFMAFHSVQGPRMGIPQLLQSRPQF
                     GYFGALIPQVIGLFLYVGFNIFNTIIAGTALSTVTGLNLQLSIILMAAAALVLTIGGY
                     DWIHFVQRWGTWLFLIGFGIFTVGVLMVGNPAPEQVTGEFAWTPFLVVIVVVASYQVS
                     QAPYVSDYSRYLPKSSTVRSTFLWSYFGSIAGSFWMIALGAFLLASFPAAASIDAVAA
                     GGNAIFNGFGTIILAVALVGLVSVIALNLYSGSLAMLASVDTVKPIRASVTTRAIAIG
                     VILVLGTAGSLLLPEDFLGSYNVFLVILLYSLVPWTAINLVDFFFVRRGDYAILEIFK
                     KN"
     gene            1269..>1594
                     /locus_tag="VHH53_14930"
     CDS             1269..>1594
                     /locus_tag="VHH53_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX2291446.1"
                     /translation="MTQTLPDTPATQHRRLDPTERHMVELLQEDGRLSVAELARELNV
                     TEVTARRKLKRLLSDGVIRVVATVDPFDVGYETPVIIGLKVERGKLDAVASAISRYPQ
                     VRYVGA"
BASE COUNT          319 a          501 c          534 g          240 t
ORIGIN      
        1 cgttcttctt gaagatttcc aggatggcgt aatcgccgcg tcggacaaag aagaagtcca
       61 ccaggttgat ggcggtccag gggaccagcg agtagagcag gatcaccagg aagacgttgt
      121 agctgcccag gaagtcctcc ggcaggagca ggctgccggc ggtgcccagg accaggatca
      181 cgccgatggc aatggcgcgc gtcgtgacgc tggcccggat gggcttcacg gtgtccaccg
      241 aggccagcat ggccagcgag ccgctgtaca ggttcagcgc gatgaccgac accaggccca
      301 ccagcgccac ggccaggatg atcgtgccga agccgttgaa gatcgcgttc ccgccggccg
      361 ccaccgcgtc gatggaggct gccgccggga agctggcgag caggaaggcg ccaagggcga
      421 tcatccagaa ggagccggcg atggagccga agtagctcca caggaacgtt gatcgcacgg
      481 tcgacgactt cgggaggtat cggctgtagt ccgatacgta cggcgcctgc gacacctggt
      541 agctggccac cacgacgatg acgaccagga acggcgtcca cgcgaactcc ccggtgacct
      601 gctcgggggc agggttgccc accatgagga cgccgacggt gaagatgccg aacccgatga
      661 ggaacagcca ggttccccag cgctgaacga agtggatcca gtcatagccg ccgatggtca
      721 gcaccagcgc cgcggcggcc atgaggatga tcgagagctg gaggttcagc ccagtgacgg
      781 tcgagagcgc cgtcccggca atgatcgtgt tgaagatatt gaagcccacg tacaggaaca
      841 ggccgatgac ctgcgggatc agcgccccga agtagccgaa ctggggccgc gattggagga
      901 gctgcgggat gcccatccgc ggcccctgca cggagtggaa ggccatgaag aaggtgccga
      961 acagctcgcc caggatgatc gcgaccagca cccacaccag cggcagcccg aggcccatgg
     1021 cgatcagtcc tacggccagg gtcgcgaggt tggcattccc caggaaccag agcgtgaact
     1081 ggtcggtgac cttgccatgc cgttccgact gggggatgta gtcgatcgag tggacctcga
     1141 ccacgctggt gggtgccgct gccctggtcg cttcggcgct catgctggct gcctccgtcc
     1201 gcccccgcga aactatgcga ttgagatatt ctgcgggtag attctatccg tatcagtcaa
     1261 ccggaacgat gacacagacg cttcccgata ctcccgccac ccagcaccgt cggctggatc
     1321 cgaccgagcg ccacatggtc gagctgctcc aggaggatgg ccgcctgtcc gtcgccgagc
     1381 tggctcgcga gctcaacgtc accgaggtga ctgcacggcg gaagctcaag cggctgctca
     1441 gcgacggcgt catccgcgtg gtggccaccg tcgatccctt cgacgtgggc tacgaaacgc
     1501 cggtgatcat cgggctcaag gtcgagcgcg ggaagcttga cgccgtggcc tccgccataa
     1561 gccgctaccc gcaggttcgc tacgtcggcg caag
//
LOCUS       DATADN010001166         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_285218, whole genome shotgun sequence.
ACCESSION   DATADN010001166 DATADN010000000
VERSION     DATADN010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285218"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1284)
                     /locus_tag="VHH53_14935"
     CDS             complement(115..1284)
                     /locus_tag="VHH53_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291447.1"
                     /translation="MAVQGLWQVLVALRPDYADNPFLYRPQLYQLAAAALAGLAVLAW
                     YVLLRRRLGPAALACGPLAWLALLSVLSAWLAPTTSHLVTLPALFLAGGGLVALLVSR
                     RSVWPVVAITVGVVPSVVLLMAVGVATFDAAGLAAGGAAAVLLALFGLTLLPLVELLL
                     PPVEQPLGKRRAVLVAVAGMGLVLALTGAGLAVDPVDAAHPRRAHLAYVLDTDTGLAR
                     WVSRDTEPAEWTRAYVTERSESAVVGFENGPVWTGPAPAVPVPTPEVTLRSRQGGTVE
                     LHVSSPRSAPAVVLRIDHRIDEITVTAPGLVRAAATLVGTGQGRWPTEVRFGDLPPEG
                     IDLTLRTTHRGALRISVYDRTRGLAELPGFRPRPQTLMPWRSDSDAVVVARTYVF"
     gene            1356..>1571
                     /locus_tag="VHH53_14940"
     CDS             1356..>1571
                     /locus_tag="VHH53_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291448.1"
                     /translation="MPGQRDHTGEHDQTEQRQRPHQSGRIDDHGSEEAEEDHIVVGGQ
                     GGQIAVSQRAGEPEHVRAVVLQAGPVEV"
BASE COUNT          277 a          527 c          578 g          189 t
ORIGIN      
        1 gctgttctcg ttgggcccgc gtggcgggca cgtgccgcca tcgtggcgta cagcgtggcc
       61 cgacacgccg gtggtgggca accgatttcg ggggctatgc cggggttccg gggctcagaa
      121 cacgtaggtc cgcgccacga caacggcgtc gctgtccgag cgccatggca tgagggtttg
      181 tgggcggggc cggaatccgg ggagctcagc cagcccacgg gtccggtcgt agacggagat
      241 ccgcagtgca ccccggtgtg ttgtgcgcag cgtgaggtca atgccctcgg gcggcaggtc
      301 gccgaagcgc acctcggtgg gccatcgacc ttgcccggtg cctacgaggg tggcggccgc
      361 gcggacgagc ccgggtgcag tgacggtgat ctcatcgatg cggtggtcga tccgtagcac
      421 caccgccggg gcggagcgcg gtgaggagac atgcagctcg acagtgccgc cctgccggga
      481 ccgcagggtc acctcgggag tgggcacggg cacagcgggg gccgggcctg tccacaccgg
      541 accgttctcg aagccgacca cggcggactc ggaacgttcg gtcacatagg cgcgagtcca
      601 ttcggctggt tcggtatccc ggctcaccca gcgagccaga ccggtgtcgg tatccagcac
      661 ataggccagg tgcgcgcggc gtggatgggc cgcatcaacc gggtccaccg ccaagccggc
      721 gccggtcagg gcgagcacca ggcccattcc ggcaaccgca accagcacgg cgcgacgctt
      781 gccgaggggc tgctctaccg gtggaaggag caactcgaca agtggcagca gggtgagccc
      841 gaacagggcc agcagcaccg cggccgcccc gcctgctgcc agccccgcgg cgtcgaaggt
      901 ggccacaccc accgccatca gcagcaccac ggacgggacg acaccgacgg ttatcgccac
      961 caccggccat accgaacgtc gcgataccag cagggcgacc aacccgccac cggccaggaa
     1021 cagcgcgggc aaagtgacca ggtgcgacgt tgtgggtgcg agccaggcgc tgagtacgct
     1081 gagcagcgcc agccacgcca gcgggccgca ggccagcgcg gccgggccga gccggcgccg
     1141 cagcagcacg taccaggcca gcaccgcgag tccggccagc gcggcggcgg ccaactggta
     1201 gagctgcggg cgataaagga acgggttatc cgcgtagtcg ggtcgcagcg ccaccaagac
     1261 ctgccacagg ccctggaccg ccacagcggt ggtgccagcg gcaacaccac cgagacggcg
     1321 ctgagcagta gccgtggaat gctcagcagc cgctggtgcc gggccagcgc gaccacaccg
     1381 gcgagcacga tcagaccgag cagcgccagc ggccacacca gagcggtcgg atagatgatc
     1441 acgggtccga ggaagcggaa gaagatcaca tcgtggtcgg cggtcagggt ggccagatcg
     1501 ctgtttccca gcgagcgggt gagcccgagc atgttcgcgc cgtggtgctg caggctggcc
     1561 cggtcgaggt t
//
LOCUS       DATADN010001167         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_128485, whole genome shotgun sequence.
ACCESSION   DATADN010001167 DATADN010000000
VERSION     DATADN010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128485"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..467
                     /locus_tag="VHH53_14945"
                     /pseudo
     CDS             210..467
                     /locus_tag="VHH53_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037846392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(491..1069)
                     /locus_tag="VHH53_14950"
     CDS             complement(491..1069)
                     /locus_tag="VHH53_14950"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2291449.1"
                     /translation="MRDQDAWLCFADESGQLLRPPRARTWSRRGHTPRVTVRAAGSGR
                     ISLAALVCCRPGRRTRLIFRMLVHHRRKGEKKGFREKEFASLLDAAHQQLGGAVVLVW
                     DNYTHHVDAAMRELIAARRWLTVFRFPAYTPDLNPAEGVWAHLKNSLGSLAPCSIDEL
                     AALARTRMKRMQYQPGLLDGFISETGLIPAPV"
     gene            complement(1066..1560)
                     /locus_tag="VHH53_14955"
     CDS             complement(1066..1560)
                     /locus_tag="VHH53_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2291450.1"
                     /translation="MRYPDGGGLTAKQRAQREQVRFEAAELFAEGVSPPQVARRLRVS
                     RKSAYAWHARWREAGVMALRSKGPSGRPSRMSPGWREWLATELEKGPAAHGWVEDQRW
                     TLDRIATVMARRFHVRFSRAQTWRILRQMGFTVQVPQRRAAERDEDAVATWIKETWPR
                     VERR"
BASE COUNT          289 a          669 c          558 g          260 t
ORIGIN      
        1 ggtcaccgtg cagagtgccg catgctgcgt catggggtgc tccagccgcc gttgtgcaag
       61 ggggctgacg gctgggcggc gcccgggccc acggccgtca cgtcgacctg ggcagaggca
      121 ggtccggcag agccgcctca gtgcgggcga ggacgatctg gtcgaacagg gcgccgtcgc
      181 cgttgctgcg cagagcgagt tggtcgacgg gcgcccttcc cctcaccctc gcccagggca
      241 ccggctgcaa ggtcgcctgc ctgcccggac tcgcgatgcg ccggatcgcc gtcctgtatc
      301 cgggcgaggc caaaaccgac gccgaggacg ccaccgtgat cgcggacgcg gcccgcacca
      361 tgccccacac cctgcgctcc ctggaactca ccgacgagat caccgccgga ctcacggtgc
      421 tcgtcggctt cggccacgac ctcgcggccg agaccacccg cactactgag gttttcggtt
      481 cggggtgaat tcatacgggg gctgggatga gcccggtttc ggagatgaag ccgtcgagca
      541 ggccgggctg gtactgcatg cgtttcatgc gggtgcgggc cagtgcggcc agctcgtcga
      601 tgctgcacgg ggcgaggctg cccaggctgt tcttgaggtg cgcccacacg ccttcagccg
      661 ggttcaggtc cggcgtgtag gcagggaaac ggaagacggt cagccaccgc ctggctgcaa
      721 tcagctcacg catcgcggca tcgacatggt gggtgtagtt gtcccacacc agcacgaccg
      781 ccccacccag ctgctggtgg gccgcgtcca gtaatgaggc gaactccttc tcccgaaatc
      841 ccttcttctc ccccttgcga cgatggtgaa ccagcatccg gaagatgagc cgggtgcgcc
      901 ggccaggccg gcagcacacc agcgccgcca gcgagatacg tcccgatcct gccgcccgga
      961 cggtgacgcg gggcgtgtgg ccgcgccggg accaggtccg tgccctcggc ggccgcagca
     1021 gctgccccga ctcatccgcg aagcacagcc acgcgtcctg gtccctcacc gtctttccac
     1081 ccgcggccag gtctctttga tccacgtggc caccgcgtcc tcgtcgcgct cggccgcccg
     1141 gcgctgcgga acctgcaccg taaatcccat ctggcgcaga atccgccagg tctgagcacg
     1201 gctgaagcgg acgtggaagc gccgcgccat caccgtggcg atgcggtcca gcgtccaccg
     1261 ctggtcctcc acccagccgt gcgcggccgg ccccttctcc aactccgtgg ccagccactc
     1321 ccgccagccg ggactcatcc gcgacggccg cccggacggc cctttggacc gcagtgccat
     1381 cacaccagcc tcacgccagc gggcatgcca ggcataggcg gacttgcgcg aaacccgcaa
     1441 ccgccgcgcg acctgcggcg gcgacacacc ttcagcaaac aactcggccg cctcgaagcg
     1501 aacctgctcc cgctgagccc gctgcttcgc ggtcaggcca cccccatccg gataccgcat
     1561 aacaccggga tacacccact cccgagatca ccgtcaccct aggccgaaac tctcagtagc
     1621 gccggatcgc agtcgccagt gaacccaccg tgatgtgcgg tgtctcctgc agatcgcggt
     1681 cggggcgaat cattcgatgc ccaaggcccc acaccagggc gcccagtccg aacccataca
     1741 caacgaccag cccgtgcccg ttctcctggg cgaacc
//
LOCUS       DATADN010001168         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_128938, whole genome shotgun sequence.
ACCESSION   DATADN010001168 DATADN010000000
VERSION     DATADN010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128938"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..979)
                     /locus_tag="VHH53_14960"
     CDS             complement(47..979)
                     /locus_tag="VHH53_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2291451.1"
                     /translation="MASTELAAPPLRPDTGTGWRSAARRRAGTPRGRWALGRARARLA
                     VGVVTLFGLVVAAVLVPWLGQLDQSLIDYSSVRLAPSAQHPFGTDAAGRDVLLRSLAG
                     LRVSLLVAVLCAVISTLMGAVIGALSGVLGGWPDRLLMRVVDTINSVPHLLLGIIIVA
                     LYRGSLLTVVASIALTHWTGIARIVRAEVLSLRDRPFIDAAISGGATRARVLRRHLLP
                     AIVPQAALSAVLILPHAVWHETALSFLGLGLPPHLASIGNLLTEGREGVLIGAWWTVV
                     FPSALLVATTLAVAAIGGWWRDRSVPRRRSELAL"
     gene            complement(972..>1719)
                     /locus_tag="VHH53_14965"
     CDS             complement(972..>1719)
                     /locus_tag="VHH53_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2291452.1"
                     /translation="TGLFSGELGTSRAFRQPVADVITQRLPWTLLLVSVGLVIMIVIA
                     LVVGVLAAWRQGGLLDRSVTAMAHGMEGIPSFVLGLGAIALFALSLRWVPVAGLTSPG
                     APASFGEVSHHLILPALVLGVSQTPWLILHLRASLLTALAEDHVAGARARGLPEWRVV
                     LRHALPTALLPFVTLLGARVPELVTGAVLVEEIFSWPGVAEAVVTAALAVDFPMLAAL
                     GVLSTAAVLLGSLLADVAIVLADPRVATDG"
BASE COUNT          329 a          629 c          597 g          164 t
ORIGIN      
        1 aggaaccgca cggacagctc ctcgacgttc accagcgcgg gggcgatcac agcgcaagct
       61 ccgagcgccg ccggggtacc gaacggtccc gccaccaacc gccgatcgcg gccacggcga
      121 gagtggtggc caccagcagc gccgaaggga agacgacggt ccaccaagcg ccgatcagga
      181 cgccttcgcg gccttcggta aggaggttgc cgatgctggc caggtgcggg ggcaggccca
      241 atccgaggaa gctcaaggcg gtctcgtgcc acaccgcgtg cggcaggatg agcaccgcag
      301 acagcgcggc ctgcggcacg atggccggta acaggtggcg gcgcagcacc cgggcccggg
      361 ttgctccgcc ggagatggcc gcgtcgatga aggggcggtc ccgaagcgac agcacctcgg
      421 cgcgcacgat ccgggcaatc ccggtccagt gggtcagagc gatcgaggcg accacggtca
      481 gcaggctgcc ccggtacagc gccacgatga tgatcccgag cagcaggtgc ggcaccgagt
      541 tgatcgtgtc caccacccgc atcagcaacc ggtctggcca gccacccagc acgccggaca
      601 gggcgccgat cacggctccc atcagggtgg agatcaccgc gcacagcacc gcgaccagta
      661 gcgagacccg tagcccagcc aagctacgaa gcagcacgtc acggccggcc gcgtcggtgc
      721 cgaacgggtg ctgcgcactc ggcgccaacc ggaccgagct gtagtcgatc agcgactggt
      781 ccagctggcc caaccagggc accagcaccg ccgccacgac cagcccgaac agcgtcacca
      841 caccgactgc caggcgagca cgggcccgcc cgagtgccca gcggccgcgc ggcgtgccgg
      901 cgcgccggcg ggcagcgctg cgccaacccg tgcccgtgtc gggacgcagc ggcggcgcgg
      961 ccagctcggt gctagccatc ggtggccacc ctcggatcgg ccagcacgat ggcgacatcg
     1021 gccagcaggg atcccagtag caccgctgcg gtggacagca cgcccagcgc ggccagcatc
     1081 gggaagtcga cggccagcgc cgcggtgacc actgcctcgg ccacaccggg ccaggagaag
     1141 atctcctcga ccagcacggc gccggtgacc agctcaggca cccgggctcc gagcagcgtg
     1201 acgaacggca gcagcgcggt gggcagcgcg tggcgcagca ccacgcgcca ctccggcaag
     1261 ccacgtgccc gggcaccggc gacgtggtcc tcggccagcg cggtcagcag ggaggcccgc
     1321 aggtgcagga tcagccacgg ggtctgcgac acgccgagca ccagcgcggg caagatcaga
     1381 tggtggctga cctcaccgaa gctggcgggg gcgccagggc tggtcaggcc ggccaccggg
     1441 acccagcgca gactcagcgc gaacagcgcg atcgcgccca gaccgagcac gaacgacggg
     1501 atgccctcca tgccgtgggc catcgcggtc accgaccggt cgagcagccc accctggcgc
     1561 caggcggcga gcaccccaac caccagcgcg atcacgatca tgatgaccag accgaccgag
     1621 accagcaaca gcgtccacgg cagccgctgt gtgatcacgt cagcaaccgg ttggcgaaaa
     1681 gcccgggagg tacccagctc acctgagaag agtccggtc
//
LOCUS       DATADN010001169         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_746974, whole genome shotgun sequence.
ACCESSION   DATADN010001169 DATADN010000000
VERSION     DATADN010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746974"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VHH53_14970"
     CDS             complement(<1..616)
                     /locus_tag="VHH53_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX2291453.1"
                     /translation="MSETCSQGRSIDPETCSHRSWTPERPLDLRAVLAPLRRGRGDPT
                     MRGDADATVWRASTTPAGAATLALRRDNGTVHATAWGPGATWALDGLPALLGADDDDS
                     AFVAHHTLVAQARRRMPGLRLGATYRVWDVLVPAVLEQKVTGIEARRSWRELCWRFGE
                     RAPGPGKLRVPPSPAAVRAIPDWEWHRAGVDSARRRALLAAAAVA"
     gene            complement(609..1709)
                     /locus_tag="VHH53_14975"
     CDS             complement(609..1709)
                     /locus_tag="VHH53_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX2291454.1"
                     /translation="MVLRPAAPAPLPGVEAVVLVGGLGTRMRPLTLSAPKPMLPTAGV
                     PFLTHLLGRIRAAGVRHVVLGTSYRAEVFAAHFGDGSALGLTLDYIVEDEPLGTGGGI
                     RNVAAALSEPTVLVFNGDVLSGLDPAEVVRTHRAARADVTLHLVRVPDPRAFGCVPTS
                     PDGEVLEFLEKTDAPPTDQINAGCYVFRREVIDSIPVGRPVSVERETFPGLLAAGARL
                     HAHVDASYWLDLGTPAAFVQGSADLVRGLAPSPELPGPVGDALLLPDATVHAQARVGG
                     GSVVGAGAVVEGGAQVYGSVLMDRVRVAGGAEVQRSVLGAGAQVGAGAVLHDAVVGDG
                     AVVGARCELRNGLRVWPGVVLPDDGVRFSADV"
     gene            1841..2089
                     /locus_tag="VHH53_14980"
     CDS             1841..2089
                     /locus_tag="VHH53_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291455.1"
                     /translation="MDALERAEQVLARARARRADIITPDSATSPMDQANTVQIPLALV
                     ALPDLQAAQPDDTIIVPNAVFSRAQRAHRDAGPTHPHR"
BASE COUNT          433 a          938 c          821 g          323 t
ORIGIN      
        1 gtgccaccgc agccgccgcc agcagggccc gccgccgcgc ggaatccacc ccggcgcggt
       61 gccactccca gtccgggatt gcgcgcaccg ccgccggact gggcggtacc cgcagcttgc
      121 cggggcccgg cgcccgttct ccgaaccgcc agcacagctc gcgccaggag cgccgagcct
      181 cgataccggt gaccttctgc tccagcacgg cgggcaccaa cacatcccac acccggtagg
      241 tggcacccaa gcgcaggcct ggcatccggc gccgagcttg cgcgacgagc gtgtggtgcg
      301 cgacgaaggc actatcgtca tcgtcggcac cgagcagcgc gggcagcccg tccagcgccc
      361 aggtcgcgcc cggtccccac gcggttgcgt gcacggtgcc gttgtcccgc cgcagcgcca
      421 gggtggcagc acccgctggc gtggtgctgg cgcgccacac ggtggcgtcg gcgtcccccc
      481 gcattgtggg atcgccccgc ccgcggcgca gcggggccag cacagcgcga agatccagtg
      541 ggcgctcggg cgtccaggac cggtgcgagc aggtctcagg atcaatgctt cgaccctgcg
      601 agcaggtctc agacatcggc ggagaagcgc acgccatcgt caggaagcac gacccccggc
      661 cagacccgca ggccattgcg caactcgcag cgcgcgccga ccaccgcgcc gtcgccgacc
      721 accgcatcgt gcaggaccgc acccgccccc acttgcgcac ctgcgcccag cacgctgcgc
      781 tgcacctctg caccgccggc gacgcgcacg cggtccatca gcaccgagcc gtacacctgc
      841 gctccaccct ccaccacggc ccccgcaccc accaccgaac cgcctccgac ccgcgcttgg
      901 gcatgcacgg tagcgtccgg cagcaacagc gcgtccccca ctggcccggg tagctcgggg
      961 gagggagcca acccgcgcac gagatcggcg gagccctgca caaaggccgc tggagtgccc
     1021 aggtccagcc agtaggaagc gtccacgtga gcgtgcaggc gagccccggc cgccaacaac
     1081 cccgggaagg tctcgcgctc caccgacacc ggacgcccga ccgggatcga gtcgatcacc
     1141 tcccgacgaa acacgtagca accggcgttg atctggtcgg tcgggggggc gtcggtcttc
     1201 tccaggaact cgagcacttc accatccgga ctggtcggca cgcagccgaa agcccgagga
     1261 tccggtactc gcacgaggtg cagcgtcaca tctgcccgtg cggcgcgatg ggttcgcacc
     1321 acctcggccg ggtccaaacc ggacagcacg tcaccgttga acaccagcac agtgggctcg
     1381 ctcaacgcag ctgcaacgtt gcggatcccg cctccggtgc ccagcggctc gtcctcgacg
     1441 atgtagtcca atgtcaagcc caatgcggac ccatcaccga aatgggcggc gaacacttcg
     1501 gcacggtacg aggtgcccag caccacgtgc cgcactccag cggcgcggat ccgtcccagc
     1561 aggtgggtaa ggaacggcac ccccgcagtg ggcagcatcg gtttgggcgc ggacagggtc
     1621 agtggccgca tccgggtgcc caaacctcca accagcacca cggcctcgac accgggcagc
     1681 ggtgccggcg cggcaggacg taacaccatg cactatctcc tcgacggttt agcacctatc
     1741 gcgggtggcg actagtgtgg ccgccgaatg cttcgacgcg tgcggcgggc caccgaggcg
     1801 gtgcacacgt ggcgacgcag cagcgacagg aggcgcaccc gtggacgctc tcgagcgcgc
     1861 ggagcaggtg ctggcccgtg cacgggctcg ccgggccgac atcatcactc ctgattcggc
     1921 gacctcgcca atggaccagg ccaacaccgt gcagatcccg ctggcgctcg tggctttgcc
     1981 ggacctgcaa gcggcccaac ccgacgacac gatcatcgtg ccgaacgctg tcttcagccg
     2041 ggcgcagcgt gcacaccgag acgccggccc gacgcatcca caccgctaaa cgatcggcgc
     2101 atccagggcg ttgctcgaca tctcagccgc aagcgtgggc cgagcacggc ggcaaggttc
     2161 cgcccatgcc gctggtgtaa ggggaaccgc ctgccgaaag ccaacctggt gagcgaactt
     2221 cacagatgtt gagctgcggt tcggtacgac ctcggccggt cattgcctcg tgatgggagc
     2281 gcccggaggc ggggtagtgg gccaaatggg tgacagcaga ggtgagtacg gtgctcgacc
     2341 gtgatagttg atcgacgtcg agcggctgca tgtaacaccc cgtcgagtcc gcctcacagc
     2401 catcgtcgct gctgctggac tctggccctg ccgggatcca gcgtgcccgc gtcgacagct
     2461 gccgacccct caagcggtgc ccaggaagcg tggcgacgag gttctggcgg acccc
//
LOCUS       DATADN010001170         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1339299, whole genome shotgun sequence.
ACCESSION   DATADN010001170 DATADN010000000
VERSION     DATADN010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339299"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..352)
                     /locus_tag="VHH53_14985"
     tRNA            complement(274..352)
                     /locus_tag="VHH53_14985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(313..315),aa:Thr,seq:cgt)
     gene            complement(677..1108)
                     /locus_tag="VHH53_14990"
     CDS             complement(677..1108)
                     /locus_tag="VHH53_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291456.1"
                     /translation="MSDQPRTGVEAIAIGLDDFVDDLRRGDAALEEPATPHALMVVAL
                     GAFLDSIGRLRKDEVAILFTEADEFTELEAADHPQVAAVYRCLATIYGYPACNARSRR
                     LRPRRGPQSRRWARCSAPSTGSRPLATPRRASSRFASRQAA"
     gene            complement(1105..1269)
                     /locus_tag="VHH53_14995"
     CDS             complement(1105..1269)
                     /locus_tag="VHH53_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291457.1"
                     /translation="MLAALAVLHRLGLTAGGVMEIAGPYKHRLLAYIAAMNDQVRYPE
                     REPWVEESRR"
     gene            complement(1343..1525)
                     /locus_tag="VHH53_15000"
     CDS             complement(1343..1525)
                     /locus_tag="VHH53_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291458.1"
                     /translation="MVPTRAIDYILVRCDAHGPILAVTACASLFDRPVNEIWASDHFG
                     VVADLAVATTTNSQPG"
     gene            complement(1528..2073)
                     /locus_tag="VHH53_15005"
     CDS             complement(1528..2073)
                     /locus_tag="VHH53_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291459.1"
                     /translation="MITDPLVSAVEPLDGWLFPLGVLVLLSAFKLIDRLLPTFEGGGF
                     DERARFYVGSWGMFGLGCLVAFITMSVSVALTVLVPLVTRGVFRREQALPYIAGANVT
                     TLGDTLVAAILLGSLDAVRVVVAQVLVVAAVTLLLLAFAWRLVGRLALSASDHALASR
                     PRLAGFIAALFCVPIALIMLR"
BASE COUNT          382 a          695 c          741 g          291 t
ORIGIN      
        1 ggcgatggac gagccgacgc ggcgtgaccc gcgcctgggc ggcactgtcg gacgccgatt
       61 gacctatgac agaccggctt cggatgaccg gtgatggtcg tatccggctg ccggccggag
      121 cggtcgcgga cggtcacccc cggggctcgc ggacagcgcc ggcggacgtc tcgtgcctgc
      181 tggcggagtt ggtcacctga accagcctgc gttgccaggt aagcggccct aatctgcggg
      241 accgcgtagc gccaccgcta cttgaacgtg aaatggagcc cgaggtcgga tcgaaccgac
      301 gaccttccga ttacgagtcg aaacgcaccc gtccagcagg taccagccag gccggttcta
      361 gctgctcagg tcagcggggt cgtctgctga gtgcgctcct gaccagcaga gttacggcag
      421 gtggagtgac caagggaatg accagcctgg tcacggccga ccaccgggcg gtggtctacc
      481 ttccgatccg aatcggaaga tagcagtctg ccgagaagcg accgccagct ccgcagattg
      541 gccatcgagc aggctccttc cgacgcaaaa tcggatgcca aggcaagcgg ctgcctaatt
      601 ggtagccttc ccttgcttgc caccgactgc ctggccaggg gccgcagagt gggttgatgg
      661 cgggcgggca gcttgatcag gccgcctggc ggctggcaaa gcgagagcta gctctcctcg
      721 gcgtcgctag gggccgggag ccggtggatg gcgcgctgca tcgcgcccat cgcctcgact
      781 gcggcccgcg tctcggccgc agtcgcctgc tcctggcgtt gcaggccgga tagccgtaga
      841 tggtggccag gcaccgatag acggcggcga cctgcgggtg gtcggccgcc tcaagctcgg
      901 tgaactcgtc ggcctcggtg aacaggatgg cgacctcatc cttcctcagc cggccgatac
      961 tgtcgaggaa cgcgccgagg gcgaccacca tcaaggcgtg cggcgtcgct ggctcctcca
     1021 gcgcagcatc accgcgcctc aggtcgtcta cgaagtcatc gagaccgatg gcgattgcct
     1081 cgaccccggt gcgaggctgg tcgctcaccg gcggctctcc tcgacccacg gctcgcgctc
     1141 ggggtagcgc acctggtcgt tcatggcggc gatgtaggca agcagccgat gtttgtaagg
     1201 accagcgatc tccatgaccc ccccggcggt gagtcctaaa cgatggagaa cggccagcgc
     1261 ggccagcaac gctgcatcgc tccgacgggc ggaagccgcc cggtgggcgg caccgcgacg
     1321 gacgagatcg tcgccccagc gactagcccg gctgggagtt cgtggtcgtg gcaacggcaa
     1381 ggtcggcgac aacgccgaag tggtcgctgg cccagatctc attgaccggc cggtcgaaca
     1441 ggctggcgca ggcggtgacg gcaaggatgg gaccgtgcgc gtcgcagcgg accaggatgt
     1501 agtcgatcgc ccttgtggga accacaccta gcgcagcatg atcagggcga tcggcacgca
     1561 gaacagcgcg gcgatgaagc cggccaggcg gggtcggctg gccagcgcat ggtccgacgc
     1621 gctgagcgcc agccggccga ccaggcgcca ggcgaacgcg agcagcagca gggtcacggc
     1681 cgcgacgacg agcacctgcg cgaccaccac gcgaacggcg tccaggctgc caagcaggat
     1741 ggcggcgacc aacgtatcac cgagcgtcgt gacgttggcg ccggcgatgt agggcagcgc
     1801 ctgctcgcgc cggaacaccc ccctggtcac cagcgggacg agcacggtga gggcgaccga
     1861 caccgacatc gtgatgaagg cgaccaggca tcccaggccg aacatgcccc acgagccgac
     1921 gtagaagcgt gcgcgctcgt cgaagccgcc gccctcgaag gtcggtagca gccggtcgat
     1981 cagcttaaag gcggacagca gcacgagcac cccgagtggg aacagccagc catcgagcgg
     2041 ctcgaccgcc gacaccagcg ggtcggtgat catcccgatc aggtccagga agaacggcgg
     2101 cgggccgag
//
LOCUS       DATADN010001171         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_747402, whole genome shotgun sequence.
ACCESSION   DATADN010001171 DATADN010000000
VERSION     DATADN010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747402"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VHH53_15010"
     CDS             <1..1434
                     /locus_tag="VHH53_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291460.1"
                     /translation="APWFGLIAVLLAIVMFRSIWRGDLATVSKRAAWALAGIWLAAAT
                     YLTPLLYTQVLDGVLVEGSNQIRTGFLDEVGIEQRDALPTLLHDQIIYRNWLRGEFGS
                     PDGPQAVQLGRDLVRAQAFTKQEIADGADNPESGVAERKKQEYEAIAARTGSAYGHFQ
                     GVDGNRIGVGFLAAVQSLMFGLFQFLAQAALLLAQILLRVVILLGPVIGLIAILYHEL
                     LRGVGRVVGAALLNVVVLSALAALHTLVMVWVFNPSRNISVLAEMLLAGMITLVMLLV
                     SRPVRRMWQMMELSVGAVGGALPAAPPGVLARLRRRKAGVESTPSERFWEETREAEVD
                     QDAPAEPVSGGRLRPEAAVPVVVVADRSDRRGGGLATPVLGPRSPDGPGADGEASPSR
                     PDEIGSRPVPAAALPTGFGSAASRRTRLGEEPVYVPSASGGDLRAPRQAELEEVAGRP
                     VWVVYRPSAGLEVGVEAADGDSARATD"
BASE COUNT          211 a          522 c          581 g          285 t
ORIGIN      
        1 gcaccctggt tcgggttgat cgcggtgctg ttggcgatcg tgatgttccg gtccatctgg
       61 cgaggcgacc ttgccacggt cagcaagcgg gcggcgtggg cgctggccgg catctggctg
      121 gccgcagcga cctacctcac cccgctgctg tacacccagg tgcttgatgg ggtgctggtc
      181 gagggcagca accagatccg caccgggttc ctggacgagg tcggtatcga gcagcgcgat
      241 gcgctgccga ccctgctgca cgaccagatc atttaccgta actggctgcg tggggagttc
      301 ggttccccgg acggaccgca ggctgtccag ctgggccggg acctggtccg cgcgcaggcc
      361 ttcaccaagc aggagattgc cgacggcgcg gacaacccgg aatccggcgt cgcggaacgg
      421 aagaagcagg agtacgaggc aatcgccgcc cggaccggca gcgcctacgg tcacttccaa
      481 ggcgtggatg gcaaccggat cggcgtcggg ttcctggccg ctgtgcagtc gctgatgttc
      541 ggcttgttcc agtttctcgc ccaggcagcg ttgctgctgg cccagattct gctgcgagtg
      601 gtcatcctgc tgggcccggt catcggcctg atcgcaatcc tgtaccacga actgctgcgt
      661 ggcgtcgggc gcgtcgtcgg tgccgcgctg ctcaacgtcg tcgtgctttc ggcgctggct
      721 gcgctgcaca cgttggtcat ggtgtgggta ttcaacccct cccgcaacat ctcggtgctc
      781 gccgagatgc ttctcgccgg aatgatcacg ctggtgatgt tgctggtgtc ccggccggtc
      841 cgccgcatgt ggcagatgat ggagctgtcg gtcggtgcgg tggggggtgc acttcccgcc
      901 gccccaccgg gtgtgctggc ccggctgcga cggcgcaagg caggtgtgga gtccactccg
      961 tccgagcggt tctgggagga gacccgcgaa gccgaggttg atcaggacgc accggcagaa
     1021 ccggtatccg ggggtcgcct ccgaccggag gcagccgtac cggtggtcgt cgtggccgac
     1081 cgatcagacc gccgtggtgg tggtctggcc accccggtgc tcggtccccg cagccctgat
     1141 gggcctggcg cagacggtga ggcgtcaccg agccggcccg acgagatcgg atcccgacca
     1201 gttccggcgg cggcgctgcc gaccggattc ggttcggccg catcccgtcg cactcggctc
     1261 ggtgaggagc cggtctatgt gccgtcggcg tcgggtggtg atctacgcgc cccccggcaa
     1321 gccgagctgg aggaggtggc cggccggccg gtgtgggtgg tgtaccggcc ctcggctggc
     1381 ctcgaagtcg gcgtggaagc cgctgacggt gacagcgccc gggccacgga ctgagcgaag
     1441 gaggtcaacg cagtgccgat tcgcacccac cgtggccgcg cggccgtcta ccgcgcgttg
     1501 tggggttggc cgctgcgttc ccccaggcac ctcgccggca ctgtgcttgc gctcgtcgcg
     1561 atcgccgtcg gcctcgggat tgccctgccc gacagcatg
//
LOCUS       DATADN010001172         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_887593, whole genome shotgun sequence.
ACCESSION   DATADN010001172 DATADN010000000
VERSION     DATADN010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887593"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VHH53_15015"
     CDS             complement(<1..1076)
                     /locus_tag="VHH53_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX2291461.1"
                     /translation="MQTHEIRQRFTDHFGRSGHTVVPSASLLVDDPNLLFVNAGMVPF
                     KPYFLGQATPPYSRATSIQKCVRTPDIDEVGKTTRHNTFFQMAGNFSFGDYFKQGAIE
                     YAWQLVTGSIDSGCYGFDPDRIWVTVYADDDEAEQLWRTIAGIPTERIQRRGMDDNFW
                     SMGVPGPCGPSSEIYFDRGPEFGRPGGPIVDEDRFLEIWNLVFMQDERGESTGPGKGD
                     FPILGPLPAKNIDTGMGVERVALLLQGVPNVYETDLLRPVITRAEQLSSRRYGQDPTD
                     DVRFRVIADHARTSVMLIGDGVTPGNEARGYVLRRLLRRTVRSARLLGVTEPVLGDIV
                     AVVRATMGPSYPEIDTDYERIEAIA"
     gene            complement(1124..1408)
                     /locus_tag="VHH53_15020"
     CDS             complement(1124..1408)
                     /locus_tag="VHH53_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291462.1"
                     /translation="MKRMFWFGLGLAAGVVANRRAAAAARRMTPAGAAENVGYAVREL
                     AAAVGSFGAEVRAGMAEREAELHATVTQRTGIDALPGLTGTRAGRTSARA"
     gene            complement(1405..1818)
                     /locus_tag="VHH53_15025"
     CDS             complement(1405..1818)
                     /locus_tag="VHH53_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX2291463.1"
                     /translation="MSAGQIAALIAAGAFVVLVGLLAVPLIKLGRTLDEATLAIRKAS
                     DEADPLFNGANTTVTHVNAQLERLDGITANAQAVTGNVSALTSLFTATLGGPLVRVAA
                     LSYGVSKAMRTRRKSRKREERAARQRRGRRSGGRR"
     gene            complement(1996..2250)
                     /locus_tag="VHH53_15030"
                     /pseudo
     CDS             complement(1996..2250)
                     /locus_tag="VHH53_15030"
                     /inference="COORDINATES: protein motif:HMM:NF018031.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron reductase"
     gene            complement(2313..3269)
                     /locus_tag="VHH53_15035"
                     /pseudo
     CDS             complement(2313..3269)
                     /locus_tag="VHH53_15035"
                     /inference="COORDINATES: protein motif:HMM:NF016099.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IucA/IucC family protein"
BASE COUNT          554 a         1076 c         1105 g          542 t
ORIGIN      
        1 gcgatcgcct cgatccgctc gtagtcggtg tcgatctccg ggtacgacgg gcccatcgtg
       61 gcccgcacca cggcgacgat gtcgccgagc accggttcgg tcacgccgag caggcgagca
      121 gaccgaacgg tgcgtcgtaa cagcctgcgc agcacatagc cacgagcctc gttgcccggt
      181 gtcactccat cgccgatgag catgacgctg gtgcgggcgt ggtcggcgat cacccggaac
      241 cgcacgtcat cggtgggatc ctggccgtag cgacggctgg agagctgctc ggcgcgggtg
      301 atcaccggac ggagcaggtc ggtctcgtag acgttgggca caccctgcag gagcaaggcc
      361 acccgctcga cgcccatccc ggtgtcaatg ttcttcgcgg gcagcggtcc gaggatcggg
      421 aagtcgccct tgccgggtcc ggtggactct ccccgctcat cctgcatgaa aacgaggttc
      481 cagatttcca ggaatcgatc ctcatcgacg atcgggccac cggggcggcc gaattccggt
      541 ccgcggtcga agtagatctc cgaggagggg ccgcacggtc cgggcacgcc catggaccag
      601 aagttgtcgt ccatgccgcg acgctggatg cgctcggtgg gtatcccggc gatcgtgcgc
      661 cacagctgct cggcttcgtc gtcgtcggca tacacggtga cccagatgcg gtctggatca
      721 aagccatagc agccggagtc tatggagccg gtcaccaact gccaggcgta ctcgatggcc
      781 ccctgcttga agtaatctcc gaaggagaag ttgccggcca tctggaagaa agtgttgtgc
      841 ctggtggtct ttccgacctc atcgatgtcc ggggtccgca cgcacttctg gatagaggtt
      901 gcgcggctgt agggaggcgt cgcctggccg aggaaatacg gcttgaaggg caccatgccg
      961 gcattgacga aaagcaggtt cgggtcgtcg accagcaacg aggcgctggg caccacggta
     1021 tgcccggagc gtccgaaatg atcggtgaaa cgctggcgga tctcgtgggt ctgcacgggt
     1081 cttcctcgaa aaagcggggt aggtggcggg tgtgaggcag ggactaagcc cgcgcggacg
     1141 ttctacccgc gcgggtaccg gtcagcccgg gaagagcgtc gatgccggta cgctgggtaa
     1201 ccgtggcgtg cagttcggct tcccgctcgg ccatgccagc ccgcacctcg gcgccgaaag
     1261 atcccaccgc tgcggccagt tcccgcactg catagcctac gttctcggcg gcgcctgcag
     1321 gggtcatccg gcgggcggcc gcggcagccc tgcgattggc tacaactccg gccgcaagac
     1381 ccaggccgaa ccagaacatt cgcttcaccg ccgacctccg ctgcgccttc ctcgtcgttg
     1441 acgtgcggcg cgttcttccc gcttgcgact cttgcggcgc gttcgcatcg ccttgctcac
     1501 gccataggac aacgctgcca cccgcaccaa cggcccaccc agggtcgcgg tgaacaagga
     1561 ggtcagcgcg gagacgttgc cggtgaccgc ctgcgcgttc gccgtgatcc cgtccagcct
     1621 ctccaactgg gcattcacgt gcgtgacggt ggtgttcgcc ccgttgaaca gcggatcggc
     1681 ttcgtcgctc gccttgcgga tcgccaacgt cgcctcgtca agggtacgcc ccagcttgat
     1741 caggggtacc gcgagcaggc caaccagcac cacgaaggcg ccggcggcga tcagcgcggc
     1801 gatctggcct gccgacacgt gccctcctca atgccgattg accggtgagc tggacgccgc
     1861 ccccggtgaa cggcggtcag gctaccgcgt ggcccggctg cacagcgggt tggataccgc
     1921 atgcaaccgc agcgtggtct ccggcagcag ccgtcgggtc gtcagttgct ccaccgcgat
     1981 ggtcggcttg aacacatcaa agagtgtgaa ccggtcggac agctcaggaa aacgctggtg
     2041 ataggcctgg ataccaccgc gcaccaccgc ccagaactcc tgctcgccga acccgtagaa
     2101 gtccgccagg agcatcgcga gctcgccaag gttgatgaac agcagcgcat cgagcaggaa
     2161 gtcggtgacg aggtcgggct caccggtctc gacgatggag ttgggattcg tatggtgcgc
     2221 cggcgtcgct gcgagctccg ggcaccgcac gggtcggcca gctgagatcg ggagaatcgc
     2281 accccgtcgt gaaagtccct gaccgccagc cgtcgcgggc gtccgtgaga gtgcaccagc
     2341 accatgttct gcgcgtgcgc ctcgagggcg atgccgtgtc cctgcagcag atgcaccagc
     2401 ggcacgacgg tcaccgcaac caggtcctcg agccactgcc gtacgccgaa tgcgcgcacc
     2461 cacgaatcca ccagtggggt gccgtcgagc ccgcgtgtgg cgaggccgtt gaacggcacc
     2521 gcttgctccc cgggttccag gtggatgtgc aggctttggc gccagatgca ggccagcacg
     2581 ccgtaggtat ccgcacgcac cagttcggac accggtgcag ggtcgaccgc cacgcccatc
     2641 acctcgccga gcacgatcag cctcagctcc tcgcgaaggt agcggtcctc ggtgaccacc
     2701 cggcgcagcc agtcggagat ccgcggcgcg ttgccgacgg tgtacggggc gagcaccctg
     2761 ctggtcgagg tgttcacgat cgacaaagcc agcttgaggt aggcccattc ggggacgtcc
     2821 cggcaggcca gggtgcgcag cgactgctga gcgaggtggg catgcgggtc cttgccgagc
     2881 acgacgagct cggtgttgcg gagttgggcg gcgaagacgc ggctgacgtg ttcacgccat
     2941 tgccacgggt gcaccggcac gaacgtgtag tccaccggat cccctccggc tcggcgaatc
     3001 cgctgctgga actccttgac ggtggcgctg ccgagctgtt cggacaggaa cgtggtttcg
     3061 tgcagctcgg tggacaccga caccatcgcc gtgttccggt gagcagccag ccacaacggg
     3121 tggatggctt gggcgaactc cggtccccag gccaggttgt ccgtcaggtc gaagccgatg
     3181 cgtgacttgt aggcggggtg gtacgggtgc ccgtcggtga tggccgactc cagcgcgtcg
     3241 tagtccgtgt cgcagagccg cccgcgcaca tcaccac
//
LOCUS       DATADN010001173         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_286298, whole genome shotgun sequence.
ACCESSION   DATADN010001173 DATADN010000000
VERSION     DATADN010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286298"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..486)
                     /locus_tag="VHH53_15040"
     CDS             complement(136..486)
                     /locus_tag="VHH53_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX2291464.1"
                     /translation="MGTWCSAERFEELVAEALDLIPAKLAEAMDNVVVLVEPRNPDNP
                     GLLGLYEGVALTMRDSYYGGVLPDRITIYREALLDICSDEQELVDEVAVTVVHEIAHH
                     FGISEHRLHELGWG"
     gene            complement(486..1451)
                     /locus_tag="VHH53_15045"
     CDS             complement(486..1451)
                     /locus_tag="VHH53_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEX2291465.1"
                     /translation="MAAGAVLGALGALVAAAAPTRSGVLLTVIAPDSVTPAAAQPLRA
                     PAHTCLTWQKPDAVDASQVSCQQPHLFEVTGTLELTDFGAEAAYPDAARWEQLVTELC
                     TQRTTQALADRFDPFGRFTVGAIKPSEAGWLSGDRTLRCGVQTVGRTGTLFATTGSVL
                     QQDQSDVHPPGTCLGNDGKALGDPVDCAGPHAVELVGVVDLKGAFPDGYPDEAAQDRQ
                     LDKECTRLAKDFTGGSAVAANKGLTLFWATLRAESWRAGSTRVDCRLGAFLPDRSGFA
                     PVTGSVKGDVKVGKEPAPAAPRNPGPAPVAAPPAKPTGAAGPPPP"
     gene            complement(1529..1663)
                     /locus_tag="VHH53_15050"
     CDS             complement(1529..1663)
                     /locus_tag="VHH53_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291466.1"
                     /translation="MASAPVARLGWPELVKTLAVPRLTATFAAGLWRNYPRSAAITIE
                     "
     gene            complement(1685..>2177)
                     /locus_tag="VHH53_15055"
     CDS             complement(1685..>2177)
                     /locus_tag="VHH53_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2291467.1"
                     /translation="LFVANGIIGAPIWLVCLLLTVCAVVGNVVGYFIGFTVGPALFNR
                     PDSRLFKREHVDKTHAFFEKYGPRAIVLGRFVPIVRTFITAIAGVGRMDPRTYFTYSV
                     IGGVAWATGVTLLGFWLGRVTFVKENVETILIGIVVLSVLPIIIEVVKARRQNRRTAD
                     TGR"
BASE COUNT          343 a          765 c          727 g          342 t
ORIGIN      
        1 cggtgagccg gcgggcgggc cgctccgcgt cctgggcgat gaccggcttg tcatcgctgc
       61 ggcccacgtc caccgctgcg gtggtccccg tcgagccagg accgttggac acgtcacaga
      121 cggtatcggt tgcgcctagc cccaaccgag ttcgtgcagc cggtgctcgg aaatcccgaa
      181 gtgatgcgct atctcgtgca ccacggtcac tgcgacctcg tccaccagct cctgctcgtc
      241 gctgcagatg tcgagcaggg cctcgcggta gatggtgatc cggtcgggta gcaccccgcc
      301 gtagtacgaa tcccgcatgg tgagcgcgac cccttcgtag agcccgagca atcccggatt
      361 gtccggattc ctgggctcta ccagtaccac cacgttgtcc atcgcctcgg cgagcttggc
      421 cgggatgagg tcaagtgcct cagcgacaag ttcttcgaag cgctcggcgc tgcaccaggt
      481 gcccatcagg gcggtggggg gcccgcagcg ccggtcggtt tcgcgggagg cgccgccacg
      541 ggagccggtc ccgggttcct cggggcggct ggggcaggtt ccttgcccac cttgacgtcg
      601 cctttgacac tgccggtgac cggggcgaag ccggaacggt ccggcaggaa ggcgccgagc
      661 cggcagtcca cccgggtgct gccggcccgc cagctttccg cccgcaacgt cgcccagaac
      721 agcgtaagtc ccttgttcgc cgccaccgcg gagccgccgg tgaagtcctt cgccagccgg
      781 gtgcactcct tgtccagctg ccggtcctgc gcggcttcgt cggggtatcc gtcaggaaat
      841 gcacccttga ggtcgaccac gccgaccagt tccacagcat ggggacctgc gcaatcgacc
      901 gggtcaccca aggctttgcc gtcgttgccc aggcaggtgc cgggagggtg cacgtcggac
      961 tggtcctgct gcagcacgct gccggtggtg gcgaacagcg tcccggtacg gccaacggtc
     1021 tgcaccccgc agcgcaacgt ccggtcccca ctgagccaac cggcctcgct gggtttgatc
     1081 gcccccacgg tgaaccgacc gaaggggtcg aatcggtcgg ccagcgcctg ggtggtgcgc
     1141 tgggtgcaga gctcggtcac cagctgctcc cagcgtgcgg cgtccggata agcggcctcg
     1201 gcgccgaagt cggtcagctc cagcgtcccg gtcacctcga acaggtgcgg ttgctggcag
     1261 ctcacctggc tcgcgtcgac ggcgtccggc ttctgccagg tcaagcaggt gtgtgccggc
     1321 gcgcgtagcg gctgcgcagc ggccggggtg acggaatctg gcgcgatcac ggtgagcagg
     1381 acgccagacc tcgtcggcgc tgcagccgcc accagcgccc cgagcgcgcc cagcacggcc
     1441 ccggcagcca tcagcctgat gccacgacgg ggccggtgcc cagcagggct cactgagaca
     1501 tcgtgacgtg gtatccgtac catctcgcct actcgatcgt gatagctgcg ctgcgcgggt
     1561 agttgcgcca cagcccggcc gcgaaggtgg ccgtcaagcg gggcaccgcg agggtcttga
     1621 ccagttccgg ccaccctagc cgcgccaccg gggcgctggc caccgtttcc gcaacgatcg
     1681 gggttcaccg cccggtgtcc gccgtccgcc ggttctgcct gcgggccttg acgacctcga
     1741 tgatgatcgg cagcaccgac agcaccacga tgccgatcag gatcgtctcc acgttctcct
     1801 tgacgaacgt gacccggccc agccagaagc cgagcagggt gacgccggtg gcccaggcga
     1861 cgccaccgat caccgagtag gtgaagtagg tgcggggatc catccggccc acccccgcta
     1921 tcgcggtgat gaaggtccgc acgataggca cgaaacgccc cagcacgatc gcccgcggcc
     1981 cgtacttctc gaagaaggcg tgcgtcttgt ccacgtgctc acgcttgaag agcctggaat
     2041 ccggcctgtt gaacagtgct gggcccaccg tgaagccgat gaagtagccc acgacgttgc
     2101 ccaccacggc acacaccgtc aacagcaggc agaccagcca gatcggggcg ccgatgatgc
     2161 cgttggcgac gaacagc
//
LOCUS       DATADN010001174         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_887880, whole genome shotgun sequence.
ACCESSION   DATADN010001174 DATADN010000000
VERSION     DATADN010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887880"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..848)
                     /locus_tag="VHH53_15060"
     CDS             complement(6..848)
                     /locus_tag="VHH53_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX2291468.1"
                     /translation="MLGVAALWVLDRVVDVFRDHPVPVAGSPFDTVAPQRPASSGSPT
                     LDRITQRGKLIVAIQEAPGLAQRSPGLNDYTGFDIALLDLIARDLGVDPAATTFKPLT
                     GGSREAAVDRGEADLVLGGYEITPQRIDEVGIAGPYLTRPLRLAVPASSPVTGLDSIG
                     PGEVCVQAASAAAAALVARGVALQTRATLESCVVLLEDRVAAIAGDQATVAAVLAKAP
                     GTLHMVGEPLGTSEYGVGLPPGDQQLRERITAVLRHAIDDGTWARLYAEHLGTPVPTP
                     PELR"
     gene            complement(1049..1903)
                     /locus_tag="VHH53_15065"
     CDS             complement(1049..1903)
                     /locus_tag="VHH53_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX2291469.1"
                     /translation="MSSALYDVPGPRAQARNKAFGVLAAFGVIALVGFVAYRFWATGQ
                     FEATKWEWLLYENLQLRLLDALVATLRAFVVGAGLALVFGLLFAVARLSEHAWLRSPA
                     SAIVELFRAIPLVILIFFLYYAAPQAGLQLGVFWSLVVGLTLYNGSVLAEVFRAGVLS
                     VPRGQREAANALGLRRNQVMRAVLLPQAVRAMLPTIVSQLVVLLKDTALGFLITYQEL
                     LFEARYLGSLAEFERPIIPTALVIGAVYITLCIALSSLAGYLERRHRRNGQLPLPDRR
                     GVTAGLNA"
     gene            complement(1900..>2190)
                     /locus_tag="VHH53_15070"
     CDS             complement(1900..>2190)
                     /locus_tag="VHH53_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX2291470.1"
                     /translation="RALGFTFTQTLIAIVVPQAIRSVVPPMISVLIALLKNTTIAAGF
                     AVAEAGQITPALSERGAPLLIGMLWVAIGFAILIIPLTATQRTLEKRWSVAR"
BASE COUNT          425 a          724 c          732 g          309 t
ORIGIN      
        1 gatagtcagc gcagctctgg gggggtaggc accggagtgc caaggtgctc agcgtagaga
       61 cgcgcccagg taccgtcgtc gattgcgtgc cgcaacactg cggtgatccg ctcgcggagc
      121 tgttggtcgc ccggcggcaa accaacgccg tactcgctgg tacccagtgg ctcgccgacc
      181 atgtgcaacg tgccgggtgc cttcgccaac accgccgcca ccgtggcctg gtcaccggcg
      241 attgccgcga cacgatcctc gagcaggacc acgcaggact ccaaagtcgc ccgggtctgc
      301 agcgccacgc cacgggctac caatgctgcc gcggccgcgg aggcggcttg cacgcacacc
      361 tccccgggac cgatcgaatc caagccagtc accgggctgc tcgcaggcac ggccagccgc
      421 agcgggcgcg tcaggtaagg gcccgcgata cccacttcgt cgattcgttg tggggtgatc
      481 tcgtaaccgc cgagcacgag gtcggcctcc ccgcggtcga cagccgcctc ccggctgcca
      541 ccggtcaacg gcttgaaggt ggtggcggcg gggtccactc cgaggtcacg cgcaatgagg
      601 tcgagcaggg cgatgtcaaa gccggtgtag tcgttcaagc ctggcgagcg ctgtgccagt
      661 ccgggtgctt cctggatcgc cacaatgagc ttgccccgct gggtgatccg gtcgagggtc
      721 ggtgatccgc tgctcgccgg gcgttgtggg gcaacggtgt cgaaggggct accggccacg
      781 gggactgggt gatcgcggaa cacgtcgacg acccgatcca acacccacaa cgctgcaact
      841 ccgagcaaca ggagcagcgc cagcagacag cctagtcggc gcggtcgcaa ccatcggcgc
      901 cgtcgcaggg caggcggcat cgcccggggc cggcggtcgg gcgacggttt cggcccggct
      961 ggcagttccg gggccacacg ccacgggtcg aggtcaggca tcgcccaccg agcccggggt
     1021 catcacttgc ctgcacaccg cgacgactct aggcgttgag gcccgcggtg acgccgcgcc
     1081 ggtccggaag cggcagctga ccgttgcgcc ggtggcgtcg ttccaggtag ccggccaatg
     1141 atgacaacgc tatgcacagc gtgatgtaca ccgcacctat cacgagtgcc gtggggatga
     1201 tcgggcgttc gaattcggcg agactgccca ggtaacgggc ctcaaaaagg agctcctggt
     1261 aggtgatgag aaaacccagc gcggtgtcct tcagcaaaac caccagttgg ctcacgatcg
     1321 tcggcagcat ggcccgcact gcctggggca gcaacacagc ccgcatcacc tggttgcggc
     1381 gcagccccaa ggcattcgct gcctcgcgct gaccgcgcgg cactgacagc acgccagccc
     1441 gaaagacctc cgcgagcact gaaccgttgt agagcgtcaa gcccacgacg agagaccaga
     1501 aaacacccag ctgaagtccg gcctgcggcg ccgcgtagta caaaaagaag atcaggatga
     1561 cgagggggat tgcccggaag agctcaacga ttgcggaggc cggcgaacgc agccacgcgt
     1621 gttcagacag ccgggcgacc gcgaacagca agccgaatac aagggcaagt cccgctccga
     1681 cgacaaaggc tcgcagggtg gcgacgaggg cgtcgagcag ccgcagctgg agattttcgt
     1741 agagcagcca ctcccatttg gtggcctcga actggccggt cgcccagaag cggtatgcaa
     1801 cgaagccgac caacgcaata acaccgaagg cagctagcac gccaaacgcc ttgttccggg
     1861 cctgagccct gggcccgggc acatcgtaga gcgccgaact catcgggcga cgctccaccg
     1921 cttctccagg gttcgctgcg tggcggtgag cggaatgatc aagatcgcga aaccgatcgc
     1981 cacccacagc atgccgatga gcagtggcgc gccacgctcg gacagcgctg gagtgatctg
     2041 gcctgcctcg gccaccgcga agcctgctgc gatcgtcgtg ttcttgagca acgcgatgag
     2101 cacgctgatc atcgggggca ccacggaccg gatggcctgc gggacgacga tagcgatcag
     2161 agtctgggtg aacgtgaaac ccagcgcccg
//
LOCUS       DATADN010001175         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_747683, whole genome shotgun sequence.
ACCESSION   DATADN010001175 DATADN010000000
VERSION     DATADN010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747683"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1461)
                     /gene="dacB"
                     /locus_tag="VHH53_15075"
     CDS             complement(25..1461)
                     /gene="dacB"
                     /locus_tag="VHH53_15075"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX2291471.1"
                     /translation="MPGLGSRIGATLQLPTQRGKRVAVVVAVLVFATGAGTGVGFGAP
                     ALVRHLTADDAVVAMTPPPAPVIPHPALRPVALDAPAPTRSGVGAVLDPLVAAGGLGT
                     LSGQVVDPTTGTVLWQRDPGMALVPGSTAKLVTCAAALLALDHQARLRTTVLTGSEPG
                     TVVLVGGGDPTLSAAQPGTVTVYPGAARLDDLVDQVQAAVAGPVDRVLVDVHRYVGDS
                     MAPGWLPADVAGGYIAPIEPVMLDGARADPTQEVSPRAAEPATAAAAELARRLGADPD
                     AVALGSAPPAAAVLGVVSSPPVETLVATALSASDNVLAEVLAREVARATGAQPSFSGA
                     AQAILTLLSEHGFDVSRATLTDGSGLSTQDQIPARLLTDLLSAAADPDETQWRTAALR
                     PMLVGLPVAGSSGTLAGRYAGPTAGGRGWVRAKTGTLTGVNSLAGTVLDAEGRVLVFA
                     LLSNGPNPVAVRPKLDALAATLRSCGCR"
     gene            1560..2060
                     /locus_tag="VHH53_15080"
     CDS             1560..2060
                     /locus_tag="VHH53_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX2291472.1"
                     /translation="MEFDVTIEIPKGTRNKYEMDHKSGRIRLDRMLFTATQYPADYGF
                     IDDSLGADGDPLDALVLVQEPTFPGCLIRCRAVGMFHMRDEMGGDDKVLCVPAGDPRL
                     EHLRDIDDLDKFYRLEIQHFFEVYKALEPGKSVEGAEWVGRTEAEQEIRTSWQRARGP
                     HPHSAG"
     gene            complement(2112..>3022)
                     /locus_tag="VHH53_15085"
     CDS             complement(2112..>3022)
                     /locus_tag="VHH53_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX2291473.1"
                     /translation="QRLLGDEVGPVRRGDLRMRLLAFLARRFGSVFVLALAQRAEGRS
                     PYATDADATAAMAADERIHAEVVRGLAARGRSRVSGTFRAAVFGANDGLVSNLALIVG
                     VTGGGATANTVLLTGLAGLLSGALSMGAGEYLSVRSQRELLAASTPDPQTRHLLPHLD
                     VEANELALVYRARGLVAEEAQRRAEAVLRNHSITSTGASKGSPEDDQHEVVGTGLGAA
                     LSSFAFFASGAAVPVLPFLVGLTGAAALIAAMVLVGLALTLTGATVGLLSGGPPVRRA
                     LRQLAIGAGAATTTYALGVLFGATLG"
BASE COUNT          490 a         1073 c         1062 g          397 t
ORIGIN      
        1 catagttccg acggtaacag cgcattagcg gcaaccgcag gatcgcagtg tcgcagccag
       61 ggcatccaac ttcggacgga ccgccaccgg gttgggccca ttggacagca gggcgaacac
      121 cagcacccgc ccttcggcgt ccagcacggt gccggcgagg ctgttgacgc cggtcagggt
      181 gcccgtcttg gcccggaccc agccccgacc gccggcggtg ggcccggcgt agcgccctgc
      241 cagtgtgccg ctgcttcccg ccaccggcag tcctaccagc atcggtcgca gggcggcggt
      301 tcgccactgg gtctcgtcag ggtcggcggc cgcgctgagc aggtcggtga gcagcctcgc
      361 cgggatctga tcttgcgtcg acagaccact gccatcggtc agagtggcgc ggctgacgtc
      421 gaatccgtgc tcgctcagca aggtgagaat cgcctgcgcc gccccggaga acgacggctg
      481 cgcaccggtt gcgcgggcca cctcgcgggc cagtacctcg gcgagcacgt tgtccgatgc
      541 gctcagcgcg gtggcgacca gggtctcaac aggtggggac gacaccacac cgagcaccgc
      601 ggctgcgggt ggggcgctgc ccagcgcgac cgcgtcggga tcggctccca gccggcgggc
      661 cagttccgcc gccgcggcgg tcgccggctc cgcggctcgg ggtgacacct cctgggtggg
      721 atcggcgcgg gccccgtcga gcatcaccgg ttcgatcggc gcgatgtatc cgccggccac
      781 gtcggcgggc aaccaacccg gcgccatcga gtcgccgacg tatcggtgca cgtcgaccag
      841 gacccgatcc accgggccgg cgacggccgc ctgtacttgg tcgaccaggt cgtccagccg
      901 cgccgcaccg gggtagacgg tcaccgtgcc gggttgtgcc gcggacagcg tcggatcgcc
      961 gccgccgacc agcacgacgg tgcccggctc ggagccggtc agcacggtgg tacgcaaccg
     1021 cgcctggtgg tccagagcga gtagggcagc cgcgcaggtg accagcttgg cggtcgatcc
     1081 aggcaccaga gccatgcccg gatcccgctg ccagagcacc gtgccggtcg tcgggtcgac
     1141 cacctgaccg gacaacgtac ccagcccgcc ggccgcaacc agcggatcga ggacggctcc
     1201 gacccctgag cgggtcgggg ccggggcatc gagggcaact gggcgcaacg ccggatgcgg
     1261 gatcaccggc gcgggtggtg gcgtcatcgc caccaccgca tcgtccgcgg tcaggtgcct
     1321 caccagcgcc ggagcgccga acccaacgcc ggtcccggcg cccgtcgcga acaccaggac
     1381 ggcgacgacc accgcgaccc gcttaccgcg ctgggtcggt agctgcagcg tggcaccgat
     1441 ccgactacca agtcctggca acgagtgctc cttacctctg ctcagcactg cgtgcttgag
     1501 gaaaatagag cgttgtaggg ctgcgccgga aatggcctcg ctgcgctacc gtcgccggag
     1561 tggagttcga cgtcaccatc gagatcccca agggcacacg caacaagtac gagatggacc
     1621 acaagagcgg tcgcatccgg ctcgatcgca tgctgttcac ggccacccag tacccggctg
     1681 actacggctt catcgacgac agcctcggcg cggacggtga tccgctggac gcgctggtgc
     1741 tggttcaaga acccaccttc cccggctgtt tgatccgctg ccgggcggtc ggcatgtttc
     1801 acatgcgcga cgagatgggc ggcgacgaca aggtgctctg cgtgcccgcc ggtgatccac
     1861 ggctggaaca cctgcgcgac attgacgacc tcgacaagtt ctaccggctg gagatccagc
     1921 acttcttcga ggtgtacaag gcgctggagc cgggcaagag cgtcgagggc gccgagtggg
     1981 tcggccgcac tgaggccgaa caggagatcc gaacctcctg gcagcgagct cgcggtccac
     2041 atccgcattc agcgggctga gcggggctat caagtaccca gtaaccacgc tgagcccgct
     2101 gaatgcggta atcagcccag cgtcgcgccg aacaggacgc ccagtgcgta cgtggtggtg
     2161 gcggcgccgg cgccgatggc gagctggcgt agggcgcggc gcaccggagg gccgcccgag
     2221 agcagcccga cggtggcacc ggtgagcgtg agcgcgagcc cgaccagcac catggccgcg
     2281 atgagcgccg cagcgccggt gagccccacc aggaacggca gcacgggtac cgcggctccg
     2341 gaggcgaaga aggcaaagct cgacagcgct gcgccgagcc cggtgcccac aacctcgtgc
     2401 tggtcatcct ccgggctgcc tttcgaggca ccagtggacg tgatggagtg gttgcgcagt
     2461 actgcttctg cgcgccgctg ggcctcctcg gccaccagcc cccgggcccg gtagaccagg
     2521 gcgagttcgt tggcttccac gtcgaggtgg ggcagcaggt gccgggtctg cgggtcgggg
     2581 gtcgaggccg ccagcagctc ccgttgcgag cgcaccgaga ggtactcacc ggcccccatc
     2641 gacagcgccc ccgacagcag ccccgccaag ccggtgagca gcaccgtgtt cgcggtcgca
     2701 ccccctccgg tgacgcccac tatcagggcg aggttgctga ccaggccgtc gttggcgccg
     2761 aagacggcgg cgcggaaggt gcccgacact cgcgaccggc cccgggccgc cagcccgcgc
     2821 acgacctcgg cgtggatgcg ctcgtcggcc gccatcgcag ccgtggcgtc agcgtcggtg
     2881 gcgtagggcg accggccctc ggcacgctgg gccagcgcaa gcacgaagac cgagccgaac
     2941 cgccgggcca ggaaggcaag cagccgcatg cgaagatcac cacgacggac cggccccacc
     3001 tcgtcgccga gcaagcgttg cc
//
LOCUS       DATADN010001176         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_887932, whole genome shotgun sequence.
ACCESSION   DATADN010001176 DATADN010000000
VERSION     DATADN010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887932"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="VHH53_15090"
     CDS             <1..1564
                     /locus_tag="VHH53_15090"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX2291474.1"
                     /translation="SVGELVAVVADSYREESGLIGRRLAAVAQLLSRRTWEAEAEDPD
                     CGYMIVTGLARTSAEVGAALNLPPGIAGALVVDAEALDSRFPKILGVLCAGDTDWRTV
                     QIILKRTRSVSDLSVFAALDTRLSAAVARWQSWSRKRVIHAVDAAVRALDPDAIVERE
                     RAEDHRHFDVIPVGDGTAKVDGVIDAESAVIVEDRISALAKGVCADDPRTLAQRRADA
                     VAAMAQGVTLACGCLACTSAGVEAAADAGRGLKPVVTVIADQSTVLGDSDKPGYLEGF
                     GVIDAQVVRRIVVRATLRTLKVPTVTAEEMLRYQPTAAVERYVRARDLTCRFPGCDRP
                     AERCDLDHTIPFNHGDPNAGGFTTVNNLKCLCRQHHRLKTFVAGWRDVQLPDGTIIWT
                     APDGRMYCTAPGGVELFEGLGPPACVAPKPRRRNLTRERAKRIARRRDKNRALRPVNA
                     ARRRLEFARDHEIYHRKERNRHRNTMMAFKGHRNSTSPFMRWVNDPYEDETLPPDWQP
                     PPEPETPDEPPF"
     gene            complement(1565..>1873)
                     /locus_tag="VHH53_15095"
     CDS             complement(1565..>1873)
                     /locus_tag="VHH53_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX2291475.1"
                     /translation="TADAAATPAVADRGDEDVAAWAARQVLTAVSTELGCAAEDIDTR
                     LPLVEIGVDSIMTVALRRQLEKQTGLSLPPTLLWEYPTAAAVTDRIVELLAAADESAA
                     "
BASE COUNT          304 a          620 c          661 g          288 t
ORIGIN      
        1 ctcggtgggt gagttggtgg cggtggtcgc tgattcgtat cgcgaggagt ccgggctgat
       61 cggtcggcgg ttggcggcgg tggcgcagtt gttgtcgcgg cgtacctggg aggctgaggc
      121 cgaggatccg gactgcggct acatgatcgt gacggggttg gcgcgcacca gcgccgaggt
      181 gggcgcggcg ttgaacctgc ccccgggtat cgccggcgcg ctggtcgtcg acgccgaagc
      241 cctcgacagc cgcttcccga aaatcctcgg tgtgctctgt gcgggggaca ccgattggcg
      301 cacggtgcag atcatcctga agcgcacgag atcggtcagt gacctgtcgg tgttcgccgc
      361 gctggatacc cggctgtcgg cggcggtggc gcgctggcag tcctggtcgc gtaagcgggt
      421 catccacgcc gtcgacgcgg cggtgcgcgc gttggatcct gatgcgatcg tcgaacgcga
      481 acgcgccgag gaccaccggc atttcgacgt catcccggtc ggggacggta ccgccaaggt
      541 tgacggggtg atcgacgccg agtccgcggt gatcgtcgag gaccgaattt ctgccttggc
      601 caagggggtg tgcgccgacg atccgcgcac cctggcccag cgccgcgctg atgcggtggc
      661 ggcgatggcg cagggcgtca cgctggcgtg tggctgcctc gcctgcacca gtgccggtgt
      721 cgaggcggcc gccgatgcgg gccgtgggct caaaccggtg gtcactgtga tcgccgacca
      781 gtcgaccgtg ttgggcgaca gcgacaaacc cggttatctc gaggggttcg gggtgatcga
      841 cgcgcaggtg gtgcggcgga tcgtggtacg ggcgaccctg cggacactga aggtgcccac
      901 ggtgaccgcg gaggagatgc tgcgctacca acccaccgcg gcggtggagc ggtatgtgcg
      961 agcccgggac ctgacgtgcc ggttccccgg gtgtgaccgg cccgctgaac gctgcgatct
     1021 ggaccatacg atcccgttca accacggcga cccgaacgcc ggtgggttca ccaccgtcaa
     1081 caacctgaaa tgtctatgcc gccaacatca tcggctcaag acattcgtcg ccgggtggcg
     1141 ggacgttcag ttgcctgacg gcacgatcat ctggaccgcc ccggatgggc ggatgtattg
     1201 cacggctccc ggtggggtgg agttgttcga ggggttgggg ccgccggcgt gtgtggcgcc
     1261 gaagccgcgg cggcgcaacc tcacccgcga acgcgcgaaa cggatcgccc gcagacggga
     1321 caagaaccgg gcgctgcggc cggtcaacgc ggcgcgccgc cggctcgaat tcgcccgcga
     1381 ccacgagatc taccaccgca aggaacgcaa ccgccaccgc aacaccatga tggcgttcaa
     1441 aggccaccgc aacagcacca gcccattcat gcgctgggtc aacgacccct acgaagacga
     1501 aaccctccca ccggactggc aacccccacc agaacccgaa acccccgacg aaccaccctt
     1561 ctgactaagc ggccgactcg tccgcggccg cgagcagttc cacgatccgg tcggtcaccg
     1621 cagcggcggt ggggtactcc cagagcagcg tcggcggcag cgacagcccg gtctgcttct
     1681 ccagctgacg tcgcagcgcg actgtcatga tggagtcgac gccgatctcg accaacggca
     1741 gacgcgtgtc gatgtcctct gcggcgcaac cgagttcggt cgacaccgca gtgagcacct
     1801 gccgcgccgc ccacgcagcg acatcctcgt cgccacggtc ggcgaccgcg ggagtcgccg
     1861 ccgcatcagc ggt
//
LOCUS       DATADN010001177         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_747877, whole genome shotgun sequence.
ACCESSION   DATADN010001177 DATADN010000000
VERSION     DATADN010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747877"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VHH53_15100"
     CDS             <1..180
                     /locus_tag="VHH53_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/thioredoxin-disulfide
                     reductase"
                     /protein_id="HEX2291476.1"
                     /translation="DGRRPPGWPVERDPYYLESSLPGVLVAGDARAESVKRVASAVGE
                     GAMAVTLVHRYLEDR"
     gene            177..1607
                     /locus_tag="VHH53_15105"
     CDS             177..1607
                     /locus_tag="VHH53_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2291477.1"
                     /translation="MTTPERVSSAALRTLFLFEELEDDRLEWVAEHGHVERYAAGVEI
                     ATEGEPAEWFYILLSGALTLSRLVRGDDVEITRGDQPGVYAGATQFYLGDQVAQVYTI
                     SVRTTADSTVLALPAADFAVVFRQWYPMATHLLQGMFVGLRNADELVGQRERLLALGK
                     LTAGLTHELNNPAAAAGRATVALRERVAGMRHKLAVLAGSELGADELKHLTVIQEEIV
                     KRMPSVPELSPLQASDREDELAGWLDDNGATCGWEIAPTLVAGGVSIDDLQRVADALD
                     ASYLDSAVRWLAYTVETETLLEEIENATGRISALVGAAKQYSQMDRSSHQSIDVHDGL
                     NSTLIMLSRKIGDQVTVVKDYDSSLPKVPAYAAELNQVWTNLVDNAVDAMNRSGTLTL
                     HTARDGEQLLVEIGDTGPGIPADMHQQIFEPFFTTKPVGEGTGLGLDVSWRIVVNRHG
                     GSMRVISEPGNTRFQIRLPLTESTRH"
BASE COUNT          300 a          570 c          575 g          269 t
ORIGIN      
        1 gacggacgtc gacctcccgg gtggccagtc gagcgcgacc cctactacct ggagtccagc
       61 ctgcccgggg tgctggtggc cggagacgcc cgggcagagt cggtgaagcg ggtcgcttcg
      121 gcggtggggg agggcgcgat ggccgtcacc ctggtgcatc gttacctgga ggaccggtga
      181 ccacacccga gcgcgtcagc tccgcagcgt tgcgcacgct gttcctgttc gaggagctcg
      241 aggacgaccg gctggagtgg gtggccgagc acggccatgt cgagcgttac gccgccggcg
      301 tggagatcgc caccgaggga gagcctgcgg agtggttcta catcctgctg tccggcgcac
      361 tcacgctgtc ccggctggtc cgaggagatg atgtcgagat cacccgcggt gatcagcccg
      421 gcgtgtacgc cggagccacc cagttctacc tgggcgatca ggtcgcgcag gtctacacca
      481 tctccgtgcg caccaccgcc gacagcactg tgctggcgct gccggctgct gacttcgccg
      541 tggtgttccg ccagtggtac cccatggcta cgcatctgct gcaggggatg ttcgtcgggc
      601 tccgcaacgc tgatgagctc gtcggacagc gtgagcggct gcttgcactg ggcaaactga
      661 ccgcaggttt gacgcacgaa ctgaacaacc cggcggcggc tgcgggccgc gcgaccgtgg
      721 cgctgcggga acgggtcgcc gggatgcggc acaaactcgc tgtgcttgcc gggtctgagc
      781 tgggcgccga cgagctcaag cacctcactg tcatccagga agagatcgtc aagcggatgc
      841 cgagcgtgcc ggaactgtcc ccgctgcagg ccagcgaccg cgaggacgag ctggccggct
      901 ggctcgacga caacggcgcc acctgcgggt gggagatcgc gccgacgctg gtcgccggcg
      961 gtgtcagcat tgacgacctg cagcgcgtcg cggacgcgct ggacgccagc tacctcgaca
     1021 gtgccgtccg gtggcttgcc tacacggtcg agaccgagac gctgctggag gagatcgaga
     1081 acgccaccgg ccgcatctcc gcgttggtcg gcgccgccaa gcagtactcc cagatggacc
     1141 gctcgtcgca ccaatccatc gacgttcatg acgggctcaa ctcaactctc atcatgctga
     1201 gccggaagat cggggaccag gtcacggtgg tgaaggacta cgactccagc ctgccgaagg
     1261 tgcccgctta cgccgccgag ctcaaccagg tgtggaccaa cctcgtcgac aacgccgtcg
     1321 acgctatgaa tcgttcagga accctcaccc tgcacacggc gcgcgacggt gagcaactgc
     1381 tggtcgaaat cggcgatacc ggccctggga ttcctgccga catgcaccag cagatcttcg
     1441 aacccttctt caccaccaaa cccgtcgggg aaggcaccgg cctcgggctg gacgtgtcct
     1501 ggcgcatcgt ggtcaaccgg cacggcggca gcatgcgggt gatctccgaa ccgggcaaca
     1561 cccggttcca gatacgcctt cctctcactg agtcaacacg tcactgagcc aacccggtac
     1621 tcagccgctc agcatgaatg aggtgaagaa ccggccgcgc tggcaagggc gacaaaggag
     1681 ccgccgccgc ggaatgcctc cggtgccagg gtgt
//
LOCUS       DATADN010001178         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_130135, whole genome shotgun sequence.
ACCESSION   DATADN010001178 DATADN010000000
VERSION     DATADN010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130135"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="folP"
                     /locus_tag="VHH53_15110"
     CDS             complement(<1..596)
                     /gene="folP"
                     /locus_tag="VHH53_15110"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2291478.1"
                     /translation="MVHPALPDPGRCVVMGVLNVTPDSFSDGGRYLDHDDAVAHGLTL
                     RAQGADLVDVGGESTRPGAQRVDASIEIARVVPVLRDLAAHGVPCSVDTTRAAVAEEA
                     LEAGAIMVNDVSGGLADPAMAPVMARCRVPWVLMHWRGHSDRMDELASYQDVVADVRA
                     ELVARVDAAVLAGVDPGALVLDPGLGFAKTAAHNWTLQG"
     gene            complement(596..1192)
                     /gene="folE"
                     /locus_tag="VHH53_15115"
     CDS             complement(596..1192)
                     /gene="folE"
                     /locus_tag="VHH53_15115"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX2291479.1"
                     /translation="MSQSGVDSRQAFDHARAEAAVRELLIACGEDPDREGLRETPARV
                     ARAYKEMFAGLFVDPDTVLERTFDEGHDELVLIRDIPMYSQCEHHLVPFHGSAHVGYI
                     PNAQGRVTGLSKLARLVDLYAKRPQVQERLTSQIADALVRNLQPRGAIVVIEAEHLCM
                     AMRGVRMPGSITTTSAVRGLFKTSASSRGEVLQLMMRR"
     gene            complement(1275..>2131)
                     /locus_tag="VHH53_15120"
     CDS             complement(1275..>2131)
                     /locus_tag="VHH53_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEX2291480.1"
                     /translation="GDPFLGRSAGQQPDYSLEVAHEIDEEVRALIEAAHTEAWEILNT
                     YRDILDELVVELLDKETLQRKDLERVFDRVEKRPRITAFNDFGGRTPSDKPPIKTPGE
                     LAKERGEPWPPYSESQPTPVGVLPGASDVPGGPPHGAPGQPGPNGVGPHTASQQAGDR
                     PGGEANGEVPGHPVPGSPQVGFPHPRSYPPSPRGPDAPPQGPSGGPSLPNSGPSTSGP
                     PNYGAPPGWRPATEPPAPAWRPAPPSGPPSQPTQGPSTDPSEARTDEQSGFWFGEDGG
                     RRPRDGDR"
BASE COUNT          328 a          750 c          722 g          331 t
ORIGIN      
        1 ccctgcagtg tccagttgtg cgccgccgtc ttggcgaaac cgagtccggg atccagcacc
       61 agcgcaccgg ggtccacacc ggcgagcacc gcggcgtcca cccgggccac cagctcagcg
      121 cggacgtcgg caacgacatc ctgataggag gccagctcat ccatccggtc gctgtgcccg
      181 cgccagtgca tgagtaccca cggcacccgg cagcgggcca tcaccggcgc catcgcggga
      241 tcagcgagac cgcccgagac gtcgttgacc atgattgcgc cggcttccag ggcctcctcg
      301 gctaccgccg cgcgggtggt gtccacactg cacggcacgc cgtgagcggc caagtcacgc
      361 agcaccggta ccacccgagc gatctcgatc gacgcatcga cgcgctgggc ccccgggcgg
      421 gtcgactcgc ccccgacatc gaccaggtcg gcgccctgcg cgcgcagcgt caagccgtgg
      481 gccacggcat cgtcgtggtc caggtagcgc cccccatcgc tgaaggagtc gggcgtgaca
      541 ttgaggacgc ccatcacgac gcagcggcca ggatcgggaa gcgccgggtg caccatcatc
      601 tccgcatcat cagttgcagc acctcgccgc gggatgacgc gctggtcttg aacaggcctc
      661 ggacggccga cgtggtggtg atcgatccgg gcatgcggac gccgcgcatc gccatacaca
      721 ggtgctccgc ctcgatcacg acgatggcgc cccgcggctg caggttacgt accagcgcgt
      781 cagcgatctg cgaggtgagc cgctcctgca cctgagggcg cttggcgtag aggtcgacca
      841 gccgagccag cttggacagc ccggtcaccc tgccctgggc attggggatg tacccgacgt
      901 gtgccgatcc gtgaaagggc accaggtgat gctcgcactg gctgtacatc gggatgtccc
      961 tgatcagcac aagctcgtcg tgtccctcgt cgaaggtccg ctccagcacc gtatcggggt
     1021 ccacgaacaa gccggcaaac atctccttgt aggcccgcgc gacgcgcgcc ggagtctccc
     1081 gcaacccctc ccgatctgga tcttcaccgc aagcgatcag cagctcccgc acggctgcct
     1141 cggcgcgggc atggtcgaaa gcctggcgcg agtccacgcc ggactgagac ataccggact
     1201 gggatacgcc ggactgggaa tcggcaccga cggtgctggt catcgctggg acggtaactg
     1261 ccgagtccgg tgcctcagcg atccccgtcc cgcgggcggc gaccaccgtc ctcaccgaac
     1321 cagaaacccg actgctcgtc cgtgcgcgct tcggacgggt cagtcgaggg gccctgtgtt
     1381 ggctgggacg ggggaccgct gggcggtgcg ggtcgccagg ccggagcggg cggttccgtg
     1441 gccggtcgcc atcccggtgg ggcgccatag ttcggcgggc ctgaggttga gggacccgag
     1501 ttcggcaagc taggaccacc gctgggtccc tgtggcgggg catccggccc gcgcggtgac
     1561 ggagggtagg accggggatg agggaatccg acctgcgggg agcccggcac cggatgccca
     1621 ggaacctcgc cgttggcttc ccccccaggg cgatcccccg cttgttgact cgcagtatgc
     1681 gggcccacac cgttggggcc gggctgacct ggagcaccgt gcggcgggcc accgggcacg
     1741 tcgctggcac ccggcagcac acctaccggg gtgggctgcg attcagagta cggcggccac
     1801 ggctcccccc gctccttggc cagttctccg ggcgtcttta tcggtggttt gtccgaaggg
     1861 gtgcgcccgc cgaagtcgtt gaacgcggtg atccgcggtc gcttctccac ccggtcaaag
     1921 acccgctcaa ggtccttgcg ctggagcgtc tccttgtcca gcagttccac gaccagctca
     1981 tcgaggatgt cccgataggt gttgaggatc tcccacgctt cggtgtgcgc ggcctcgatg
     2041 agcgcccgca cctcctcgtc gatctcgtgc gcaacctcca gcgagtagtc cggttgctgg
     2101 cctgcggagc ggccgaggaa cgggtcgccc t
//
LOCUS       DATADN010001179         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_130736, whole genome shotgun sequence.
ACCESSION   DATADN010001179 DATADN010000000
VERSION     DATADN010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130736"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..752
                     /locus_tag="VHH53_15125"
     CDS             243..752
                     /locus_tag="VHH53_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2291481.1"
                     /translation="MSDHLTVRLARPDELDQVGQLTLDAYVTDGYANPAGSYAAALAD
                     APRRARDADLLVAANPEGMVLGTITVCRPGSPMGELSRPGELEFRMLAVAPAARRRGV
                     GRALVVAVLQRATEIGAHRVVLCSAEKMCAAHRLYARLGFIRLPERDWQPVPGMTLLA
                     FGKTTRRPE"
     gene            complement(829..1488)
                     /locus_tag="VHH53_15130"
     CDS             complement(829..1488)
                     /locus_tag="VHH53_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2291482.1"
                     /translation="MSFLIRIQLPDRPGALGAVATALGNVDCDILSLDVVERRSGIAI
                     DDLVVELPSGRLPDVLITAAESVEGVEVDAVRPWAGVLDTHRELELVEQVAADPAGGL
                     QIFTDGVPKIIRAGWAVVLTMTQGRTHRLTGSFAAPETLAAQLPWWPLRRATILDGDD
                     PWVPLPWRELGTELAATPLGGSDRVLLVGRPGGPMFRAAEVAHLEHLAGIVAVVLRNG
                     N"
     gene            1712..2008
                     /gene="gatC"
                     /locus_tag="VHH53_15135"
     CDS             1712..2008
                     /gene="gatC"
                     /locus_tag="VHH53_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX2291483.1"
                     /translation="MPTISRDEVAHLARLARLAVTDQELDLFAGQLEVILSSVARVSE
                     VAAADIPPTSHAVGLTNVFREDVPRPGLTQQQALSGAFAAEEGRFRVPRILDES"
     gene            2005..>2401
                     /locus_tag="VHH53_15140"
     CDS             2005..>2401
                     /locus_tag="VHH53_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX2291484.1"
                     /translation="MTPLTQLPAADLASLIHTRETSAVEVVRAHLDRIAAVEPAVHAF
                     LHLDAEGALAAAAAVDECLQRGDPPASPLAGVPLALKDVFTTADMPTTCGSRILEGWR
                     PPYDATVTRLLRQAGVVILGKTNMDEFAMG"
BASE COUNT          388 a          843 c          791 g          379 t
ORIGIN      
        1 acgctgccca acggcttatc agaaggtggg ccgggccgtg gcggcatcgg ccgaggtggg
       61 cgattcccta tcaccgatag cttctattgg tgggtcacgc cgtgcctcgg cctcgtctat
      121 ggcgcgccta tctcgatccg gcgagtgtcc aagaagggat gaacaccctg cggcaacgat
      181 tatcagcaat gtccggactc gcctgatcga cgcttcgccc ggcccgtggt atcaccgcat
      241 gcgtgagcga tcacctgacc gtgcgcctag cccgtcccga cgagctcgac caggtgggac
      301 agctcaccct ggacgcctac gtcaccgatg gttacgccaa tcccgcgggt tcctacgctg
      361 ccgcgctggc cgacgcgccg cggcgagcca gggacgctga cctgctggta gcagcgaatc
      421 ccgaggggat ggtgctaggc accatcacgg tgtgcaggcc gggcagcccg atgggggagc
      481 tgtctcgacc cggtgagctg gagttccgga tgctcgcggt ggcgcccgcc gcccgccgcc
      541 gcggtgtcgg gcgagcgttg gtcgtcgccg tactccagcg ggccaccgag atcggcgcac
      601 accgggtcgt gctgtgcagc gctgagaaga tgtgcgccgc gcaccggctc tacgcgcgat
      661 tgggattcat ccggctgccc gaacgcgact ggcaaccggt accgggtatg acgctgctgg
      721 ccttcggcaa gaccacaagg cgtcctgagt aggccgtgtc ccacagatca cgcacgagtc
      781 agtgaccgaa aactatgagt actcttggga acccgggtag gccgtcgctc agttgccgtt
      841 gcgtagcacg accgcgacga tcccggccag gtgctccaga tgcgcaacct cggcagcgcg
      901 gaacatcggc ccccctgggc gacctaccag caggactcga tctgagcctc ccagcggagt
      961 cgccgccagc tcggtgccca gctctcgcca gggcaacggg acccaagggt cgtcaccgtc
     1021 caggatcgtc gcccggcgca gcggccacca tggcagctgc gcggccaagg tctccggtgc
     1081 ggcgaaagag ccggtgagcc ggtgtgtgcg cccctgcgtc atggtaagca ccaccgccca
     1141 ccccgctcga atgatcttag gcaccccgtc ggtgaagatc tgcaaaccac cggcgggatc
     1201 agcggcgacc tgctccacca gctccagctc gcggtgggtg tccaggaccc cggcccacgg
     1261 gcggacggcg tcgacctcga ctccctcgac cgattccgcc gcagtgatca acacatcggg
     1321 cagccgcccg gagggcagtt ccacgacgag gtcgtcgata gcgatgccgg atctgcgctc
     1381 gacgacgtcc aggctgagga tgtcgcagtc cacattcccc aaggcggtgg ccactgcgcc
     1441 aagagctccg ggtcggtccg ggagctgaat ccggatcaaa aaggacatcc gccgtgcctg
     1501 ttcattacgg tcctctcatc cgttcatccg ttcggtcctt acggaactcc gaccgcccat
     1561 gccgcgcgcc ggtcgccgat tgtgacagag cgagctggat attgcgccgc gcagcccatc
     1621 ggtgacaaag cgcccaggcc gtgcaggtca agcgctcacc cgcggcctag actcgccggg
     1681 tcagcacccc acgaacgcgc caggagcctg cgtgcccacc atttcccgtg acgaggtcgc
     1741 gcatctcgcc cggctcgccc ggttggccgt tactgaccag gagttggacc tgttcgccgg
     1801 ccagctcgag gtgattttga gctcggtcgc ccgggtcagc gaggtggccg ccgccgatat
     1861 cccacccacg tcacacgccg tcgggctgac caatgtgttc cgcgaggacg tgccacggcc
     1921 tgggctgacc cagcagcagg cgttatcggg tgccttcgcc gctgaggaag gccggttccg
     1981 ggtaccgcgc atcctggatg agtcatgacg ccgctgactc agttgccggc cgccgacctg
     2041 gccagcctca tccacacccg ggagaccagc gcggtggagg tggtcagagc acatctggac
     2101 cggatcgccg cggtggagcc cgcggtgcac gccttcctgc acctcgacgc cgaaggcgcg
     2161 ctcgccgccg ccgccgccgt ggacgagtgt ctgcagcgcg gggacccgcc ggcctcaccc
     2221 ctggcgggtg tgccgctggc gctcaaggac gtgttcacca ccgctgacat gccgaccacg
     2281 tgcggctcgc ggatcctcga gggctggcgc ccaccgtatg acgccaccgt gacccgcctg
     2341 ttgcggcagg cgggcgtggt gatcctgggc aagacgaaca tggacgagtt cgccatgggc
     2401 t
//
LOCUS       DATADN010001180         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_287516, whole genome shotgun sequence.
ACCESSION   DATADN010001180 DATADN010000000
VERSION     DATADN010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287516"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VHH53_15145"
     CDS             complement(<1..348)
                     /locus_tag="VHH53_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2291485.1"
                     /translation="MTVWSDGLVRSERGDDVEQLRATAVAVVQAVGDLVVRMRAAGPG
                     HVDTKSSITDVVTAADTAAEQLARRLLAQWRPGEPVLGEEEGGAGPPAGDLCWVVDPI
                     DGTVNYLYGLPWYA"
     gene            417..1169
                     /locus_tag="VHH53_15150"
     CDS             417..1169
                     /locus_tag="VHH53_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2291486.1"
                     /translation="MARTAGFGVDVGGSGVKGCTVDLRTGSLDDERVRIPTPQPSTPQ
                     AVAEAVATITGEFGWQGPMGVTLPCVLKNGIALTAANVDPSWKGTDAAGLFSDYCQVP
                     VTVLNDADAAGLAEMRFGAGRDNDGVVLLLTFGTGIGSALFVDGELVPNTELGHVQID
                     GADAEDRAAASTRDEEALSWPDWAARVSRYLQVLENLIWPDLIIAGGGISRKADRWLP
                     LVQCRTPVVAAALRNNAGIVGAAAAAAEGRVR"
BASE COUNT          282 a          600 c          541 g          256 t
ORIGIN      
        1 cgcataccag ggcagcccgt agagatagtt cactgtgccg tcgatcggat cgaccaccca
       61 gcacaggtct cctgccggcg ggccggcgcc cccttcctcc tcgcccagta ccggctcacc
      121 aggccgccac tgtgcaagaa gccgccgggc cagctgctcc gctgccgtgt cggcggcggt
      181 gaccacgtca gtgatgctgc tcttggtgtc cacgtgcccg ggcccagcgg cccgcatccg
      241 gacgaccagg tcgccgaccg cctgcaccac cgccacggcc gtggcccgca gctgttcgac
      301 gtcatccccg cgctcgctgc gcaccaagcc atccgaccac accgtcatcg acgagtccgg
      361 gttagggtcg gatccgatcg gcggtcggtg cggcgggcga tcaggagagg acacggatgg
      421 ccaggactgc cggattcggg gtggacgtcg gcggatccgg tgtcaagggc tgcactgtgg
      481 acctgcgcac cggctcactg gacgacgagc gagtgcgcat tcccaccccg cagccctcca
      541 ccccccaggc ggtggccgag gccgtcgcca ccatcaccgg cgagttcggc tggcagggcc
      601 cgatgggagt caccctgccc tgtgtcctca agaacggcat cgcgctgacc gcggccaacg
      661 tggacccgtc atggaagggc accgacgcgg ccggcctgtt ctccgattac tgccaggtac
      721 ccgtgaccgt gctcaatgac gccgacgcgg cgggcctcgc cgagatgcgg ttcggcgcgg
      781 gccgcgacaa cgatggtgtg gtcctactgc tcaccttcgg caccggtatc ggcagcgcct
      841 tgttcgtcga tggagaactg gtacccaaca ccgaactggg ccacgtccag atcgacggtg
      901 ccgacgctga ggaccgcgcc gccgcttcga cccgcgatga ggaagccctg tcctggccgg
      961 attgggccgc ccgagtatct cgctacctcc aggtcctgga gaatctgatc tggcctgatt
     1021 tgatcatcgc gggcggtggg atcagccgga aggctgacag gtggttaccg ctggtgcagt
     1081 gccggacccc ggtcgtcgcg gcggcattac gcaacaacgc cggcatcgtc ggtgccgcag
     1141 cggccgccgc ggaagggcgc gtgcggtgac tgatccacag aggctggatc cacactgctg
     1201 ggtcgaccct gcagtgtcta ctctctggct ccgagtggcg atcagcgtcg ttagaataca
     1261 gagtcgttac aatggaacac ggcatcgtcc ccgagcactc tggatggcac cgccggagtc
     1321 cgcagacctc aatggtttcc ggcactcgct ccagtaacga gctcgccgac gacaagcccg
     1381 cttctccaac agcgcccgaa caccgaccgt cgcgaaaggc cgtccgtggc agccgcagaa
     1441 cccgcatccc gtcgcgccac cgcctcgaag tcagcgacct ccaagtctgc gccctcgaat
     1501 tccggggtgg ccgaagcgcc ctcttccccg aaaggcacca ggagcggcgt ggctcggacg
     1561 cgcccgaagg ccaacagcag cgccgccaag gctgtcccga agcccgtcga cgtggctgaa
     1621 cccgtcgacg tcgatgctgt gatcgaagaa accgctgaca tcgagagcgg gcccgctgc
//
LOCUS       DATADN010001181         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_130809, whole genome shotgun sequence.
ACCESSION   DATADN010001181 DATADN010000000
VERSION     DATADN010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130809"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..429
                     /gene="arr"
                     /locus_tag="VHH53_15155"
     CDS             13..429
                     /gene="arr"
                     /locus_tag="VHH53_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HEX2291487.1"
                     /translation="MCAHRGTLYHGTKAALEPGDELLPGHGSNFQEGRVSNNVYFTAL
                     VDTAAWGAELATALAGSGQRGHIYVVEPLGPFEDDPNVTDKRFPGNPTQSYRTRHPLR
                     VVGELDSWEGHDPDVLEGMLDSLARLREQGLDVIED"
     gene            complement(554..>1644)
                     /locus_tag="VHH53_15160"
     CDS             complement(554..>1644)
                     /locus_tag="VHH53_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2291488.1"
                     /translation="FFFRPLGGILLGRFADLRGRKAGMLLTILLMAGGSMVIAILPTY
                     EQVGWLAPVLLLLARVAQGMSLGGEVSNASAYLGEIAPPAHRGRYSSFFYISTGSALL
                     AASLLGVLLTSTLSQDQLESWGWRIAFFIGGVLGLVGMWLRRSLVETEQFEENAEKAR
                     ATKHPLLQTIKHHPKAVVQLCAFTLLSTLSYYTFFSALTPFAITFRDADKTDVFIALS
                     IGTALFVALCYPFGKLSDHIGRKPQLLIWSGAMAVLIVPLSFLVRDNLLSLIIVFCTG
                     LGLYALMASIAPAIMSELFPTELRAIGIGSWYNLTVATFGGTAPLVITWLPGAGHPLW
                     FFWYVAVGAAVAFAVILTLRETKGTELR"
BASE COUNT          304 a          550 c          587 g          203 t
ORIGIN      
        1 gacctacgaa cgctgtgcgc acatcgaggg accctttacc acgggacgaa ggccgcgctc
       61 gagcccggcg atgagctgct ccccggccat ggctccaact tccaggaggg ccgggtgtcg
      121 aacaacgtct acttcaccgc gctcgtcgac acagccgcgt ggggagcgga gctggcgaca
      181 gcgctggccg ggagcgggca gcggggacac atctacgtcg tggaacccct gggccccttc
      241 gaggacgacc cgaacgtgac cgacaagcgg ttccccggca accccacgca gtcctaccgc
      301 acccgccatc cgctgcgcgt cgtcggggaa ctggacagct gggagggtca cgacccggac
      361 gtgctggagg ggatgctgga cagcctggcg cggctgcggg aacaggggct ggacgtcatc
      421 gaggactagg aggggtcctc tcccccgcag ctgacacagc tccgctctgc cgcaacgggg
      481 aacagctgtt ccgctgcggg aggcgacagc cggcaggtgc cctcacacct ccaccgtgag
      541 gaggggccgg gcctcagcgc agctcggtgc ccttcgtctc ccgcagggtg aggatgacgg
      601 cgaacgcgac cgcggcgccc accgcgacgt accagaagaa ccacagcggg tgcccggccc
      661 cgggcagcca ggtgatcacc agcggggccg tgccgccgaa cgtggcgacg gtcaggttgt
      721 accaggagcc gatgccgatg gcgcgcagct ccgtggggaa cagctcgctc atgatcgccg
      781 gggcgatcga cgccatgagc gcgtagagcc cgaggccggt gcagaagacg atgatcagcg
      841 agagcaggtt gtcgcggacc aggaaggaca gcggcacgat cagcacggcc atcgcgcccg
      901 accagatcag cagctgtggc ttgcgcccga tgtggtcgga gagcttcccg aacggatagc
      961 acagcgcgac gaacaacgcg gtgccgatcg agagcgcgat gaagacgtcg gtcttgtcgg
     1021 cgtcgcggaa ggtgatcgcg aacggcgtca gcgcgctgaa gaacgtgtag tacgacagcg
     1081 tcgacagcag cgtgaacgcg cacagctgca ccacggcctt ggggtggtgc ttgatcgtct
     1141 gcagcagcgg gtgcttcgtc gcgcgggcct tctccgcgtt ctcctcgaac tgctcggtct
     1201 ccaccagtga ccggcgcagc cacatgccga ccaggccgag cacgccgccg atgaagaacg
     1261 cgatccgcca gccccaggac tccagctggt cctggctgag ggtgctggtc agcagcacgc
     1321 cgagcaggga ggcggccagc agcgccgatc cggtggagat gtagaagaac gacgagtagc
     1381 ggccgcggtg ggcgggcgga gcgatctcgc cgaggtaggc cgaggcgttg gacacctccc
     1441 cgcccagcga catcccctgg gcgacccggg ccagcagcag caggaccggc gccagccagc
     1501 ccacctgctc gtacgtgggc aggatcgcga tcaccatcga gccgcccgcc atcagcagga
     1561 tggtcagcag catcccggcc ttgcggccgc gcaggtcggc gaaccggccg agcaggatgc
     1621 cgcccagcgg ccggaagaag aagg
//
LOCUS       DATADN010001182         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_888976, whole genome shotgun sequence.
ACCESSION   DATADN010001182 DATADN010000000
VERSION     DATADN010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888976"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..667)
                     /locus_tag="VHH53_15165"
     CDS             complement(17..667)
                     /locus_tag="VHH53_15165"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX2291489.1"
                     /translation="MSHVYDCARADSRAAGLAAAASAARAGQLVVLPTDTVYGLGCDA
                     FSSAAVQALLDAKGRGRDMPVPVLVGSWSTVDGLVLGVPSQARTLIEAFWPGGLSLVL
                     PHASSLAWDLGDTRGTVMLRMPLHPVALELLRDVGPMAVSSANRSGQPPATTVEQARE
                     QLAERVPVYLDGGTSGEPLASTIVDLTADEPVVLREGAVTVAELSEVLGLTIQVTR"
     gene            789..1721
                     /locus_tag="VHH53_15170"
     CDS             789..1721
                     /locus_tag="VHH53_15170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2291490.1"
                     /translation="MAAHRRVIVAVVATALTLLGLAGTGAVQTTTAAQTASVPERYVA
                     LGDSYAAGPEIPVQRFDPIGCRRSTSNYPSLVAAALGISDFTDVSCTGAQTENMTDPQ
                     RVPLGINPPQFNALAPDTDLVTLTISGNDIGFSDIFVTCARLSSTDPLGNPCERQATA
                     GGTDIYAQRIDAEAPNVARVLEGIRERSPRATVLLVGYLRIVPPEVGCYPVFPIARGD
                     VPYLDGIQQQLNAMLADQASDHGAVFVDSYAASLGHDACKLPGVKWVEGIAPTSPAAP
                     VHPNAIGMRAVAALALEALREPEFSDVSEVVTLR"
     gene            1803..>2000
                     /locus_tag="VHH53_15175"
     CDS             1803..>2000
                     /locus_tag="VHH53_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2291491.1"
                     /translation="MHRRAVVAAAGTALALAGLAGTGAGQATAAAHIPAAPRHYVALG
                     DSYAAGPAIPVQRTDPIGCGRS"
BASE COUNT          319 a          711 c          670 g          300 t
ORIGIN      
        1 ctggtgatca gaacggtcag cgcgtcacct gaatggtgag gccgagcact tcgctgagct
       61 cggcaacggt cacggcacct tcgcgcagca ccacgggctc gtcagcggtg aggtccacga
      121 tcgtggaggc cagcggttcg ccggaagtcc ccccgtcgag atacaccggt acccgctcgg
      181 cgagctgctc gcgggcttgc tcgacggtgg tcgccggtgg ttggccggag cggttcgcac
      241 tcgacaccgc catcggaccc acgtcgcgga gcagctccag ggcgactgga tgcagtggca
      301 tccgcagcat gacggttccc cgagtgtcgc ccagatccca ggccagtgac gatgcgtgcg
      361 gcaataccag cgacagccca ccgggccaga atgcctcgat cagtgtgcgg gcctgtgacg
      421 gtacgcccag caccaagccg tccaccgttg accaggaacc caccaggacc gggaccggca
      481 tgtcccgccc ccgccccttg gcgtccagca acgcctggac cgctgccgag gaaaaggcgt
      541 cgcaacccag cccgtagacg gtgtccgtcg ggagcaccac gagctggccc gcccgggcgg
      601 cactggcggc cgcggccagc cccgcagcac ggctgtcggc ccgtgcgcag tcgtagacgt
      661 ggctcacgga tccgcagact acgaccgctg gccggggcgg cctcgacggc gggggatgtt
      721 tgacactttg gtgatcagca aggcacgatc ccggttgcgg gggagggtcc tttcgttccg
      781 gaggttatgt ggccgcgcat cgccgtgtca tcgtcgccgt cgtagcaacc gctctcaccc
      841 tgctcggcct tgccggcacc ggagccgtcc agacgactac ggccgcccag accgccagtg
      901 tgccggagcg ctacgtggcg ctcggagact cgtatgcggc gggtccggag attcccgtac
      961 agcgattcga tccgatcggc tgccgtcgct ccaccagcaa ctacccgtca ttggtcgcag
     1021 cggcgctcgg catcagtgac ttcaccgacg tcagctgcac cggcgcgcag accgagaaca
     1081 tgacagaccc tcaacgcgtg cccctgggca tcaatccacc ccagtttaac gccttggcgc
     1141 cggacaccga cctggtcacg ctgaccatca gcggcaacga catcgggttc agcgacatct
     1201 tcgtcacctg cgcccggctc agctccaccg acccgctggg caacccgtgt gagcggcagg
     1261 ccaccgccgg cggcacagac atctacgcgc agcgcatcga tgccgaagcg ccgaacgtcg
     1321 ccagggtgct ggagggcatc cgggagcgct ctccgcgagc gaccgtgctg ctggtgggct
     1381 acctgcggat cgtgccaccc gaggtcgggt gctacccggt cttcccgatc gcccgcggtg
     1441 acgtgcccta cctggacggc atccagcagc agctcaacgc catgctcgcc gatcaagcca
     1501 gcgaccacgg cgcggtcttc gtcgattcct acgctgcctc gctgggccac gatgcctgca
     1561 agctgcccgg cgtgaaatgg gtggagggga tcgcgcctac cagcccggcc gccccggtgc
     1621 accccaatgc catcggcatg cgagcggtgg ccgccctggc gctggaggcg ctcagagagc
     1681 cggagttcag cgacgtgagc gaagtggtaa cactgcggtg acccgtcgcg ttgacagcag
     1741 gtgatgggaa cggcacgatc tgcgtccggt gggaccgttc actgtcgggg gctcatgtgg
     1801 ctgtgcatcg ccgtgcggtg gtggccgctg cgggcaccgc gctcgctctg gccgggcttg
     1861 ccggcacggg agcgggccag gcgaccgcag ccgcgcacat ccccgccgca ccgaggcact
     1921 acgtcgcact cggtgactcc tatgcagcag gtccggcgat cccggtgcag cggaccgatc
     1981 cgatcggctg cgggcgctcc
//
LOCUS       DATADN010001183         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_287856, whole genome shotgun sequence.
ACCESSION   DATADN010001183 DATADN010000000
VERSION     DATADN010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287856"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..308)
                     /locus_tag="VHH53_15180"
     CDS             complement(24..308)
                     /locus_tag="VHH53_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291492.1"
                     /translation="MTDALSFAEIDAQHLELLPARTVLSLFATGGGGAKGGDAGNAGQ
                     GGYGGEGEGKASISHNWFYLEDGYVYNTATGGQGGAADGGAATGGTGAEK"
     gene            complement(565..>1764)
                     /locus_tag="VHH53_15185"
     CDS             complement(565..>1764)
                     /locus_tag="VHH53_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX2291493.1"
                     /translation="VAHLVEGVFPAAKKSSSWLRTVTELPVALRGDRVDLPLLTLPVA
                     ADQKDLGEALSAHETAFDARRLTEERRLLYVALTRAQRSLLVSGHYWGQTGSAPRSPS
                     DFLLEVRDVEHCVIDQWAPPPGEGEPNPLDTVARQAPWPADPLGARRDPMAEGAQLVR
                     CALVSVKQRDQTVSHSRAGWQREIDVLLAERDARRAEQRVVLPAHLSVSQLVELTDDP
                     SALATRLRRPLPLAPNPMARRGTAFHAWLERRFGATRLIDHDELPGAADAGAAPNTEL
                     GALQAAFLASEWAARSPHDVEVPFETVLDGVGLRGRMDAVFADADGGWTVVDWKTGAV
                     PDDAALPAVSVQLAAYRLAWSALSGAPLTRVRAAFHYVRDGRTVRPADLLDADGLRAL
                     LRAIPAD"
BASE COUNT          317 a          613 c          566 g          268 t
ORIGIN      
        1 cccggcaaca ctccctcggt gcgctacttc tcggcacccg ttccaccggt ggcagcacca
       61 ccatcagcag ctccgccctg gccaccagtg gccgtgttgt acacgtagcc gtcctcgagg
      121 tagaaccagt tgtgagagat cgacgccttc ccttcgccct cgccgccgta gccgccctgg
      181 ccagcgttgc cggcgtcgcc gccctttgcg ccgccgccac cagtggcgaa cagcgacagc
      241 acagtacgtg ccggcagcag ctccaggtgc tgtgcatcga tctccgcaaa gctcagtgcg
      301 tcagtcatca agtcctccta ggaaaggtgg ccccggtggg gccccgggca cagaaaacac
      361 ctcatcggca agaattgccg attcagcacc acataccctg ccgaggagta aggcggagcg
      421 gcaattcgta gtcgcacgta aatcctttgg tggactacct agcagtggag tagatttctg
      481 aacacgtagt gggtagcagc attctacgga gatgttgggc actgagtacc aacactgccg
      541 tgagggccgt agccacacct cacgtcagtc ggcggggatg gcgcgcagca gagcccgcaa
      601 tccgtcagca tcgagcaaat cggcgggccg taccgtgcgg ccgtcgcgga cgtaatgaaa
      661 tgccgctcgg acacgggtca gcggggcgcc ggacagggct gaccaggcca gccggtacgc
      721 ggccagctgc acgctcaccg cgggcagcgc ggcgtcgtcg ggtactgctc ccgtcttcca
      781 gtcgaccacc gtccagccac catcggcgtc ggcgaataca gcatccatcc ggccacgtaa
      841 ccccaccccg tcgagcacgg tctcgaaagg cacctcgaca tcgtgtgggc tacgggcggc
      901 ccactcgctg gcgaggaaag cggcctgcag tgcccccagc tcagtattcg gggcggcacc
      961 ggcatcggcg gcgccgggca actcgtcgtg gtcgattagc cgggtggcac cgaaccggcg
     1021 ctccaaccat gcgtggaatg cggtaccgcg gcgcgccatc gggttcgggg ccagcggcag
     1081 cggcctgcgc agccgcgtag ccagcgccga cgggtcgtcg gtgagctcca cgagctggct
     1141 taccgacagg tgcgctggca ggacgacccg ttgctcagcg cgacgagcat cccgctcagc
     1201 gagcagcacg tcaatctcgc gctgccaccc agctcgtgag tgcgaaacag tctgatcacg
     1261 ctgtttcaca ctcacgagcg cgcagcgaac caactgcgca ccttcggcca tgggatcccg
     1321 gcgggcaccg agcggatcag ccggccaggg tgcctgccgg gcaactgtgt ccagcgggtt
     1381 gggctcgccc tcaccgggcg gcggggccca ctgatcgatc acacaatgtt ccacgtcgcg
     1441 gacctccagc aggaagtccg agggtgacct gggtgcgctg ccggtctgcc cccagtagtg
     1501 gccactgacc agcagggatc gctgggcacg ggtcagcgct acgtacagca gccggcgttc
     1561 ctcggtgagc ctgcgcgcgt cgaaagccgt ctcgtgtgcc gacagcgcct caccgaggtc
     1621 cttctgatcg gcggcgaccg gcaacgtgag aaggggtaag tcaacgcgat cgccgcgcag
     1681 cgccaccggc agctcagtga ccgtccgcag ccacgacgag ctcttcttgg ccgcgggaaa
     1741 cactccctcg accaggtgcg caac
//
LOCUS       DATADN010001184         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_131132, whole genome shotgun sequence.
ACCESSION   DATADN010001184 DATADN010000000
VERSION     DATADN010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131132"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..279
                     /locus_tag="VHH53_15190"
                     /pseudo
     CDS             73..279
                     /locus_tag="VHH53_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            456..1586
                     /locus_tag="VHH53_15195"
     CDS             456..1586
                     /locus_tag="VHH53_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX2291494.1"
                     /translation="MARSPLSPAEVDEVWDRWRSGQAVKVLARAMRRHPSTVRDLLKR
                     CGGVRAAPRRRWELRLSLAEREEISRGLAADRSLRSIAAGLGRAPSTICREVSANGGR
                     AEYRALEADRAAWLRASRPKPTKLSQGPELARVVTEKLELRWSPQQIAGWLRRGTVGY
                     VSHKTIYRSLFIQARGDLRRELTRYLRTRRAMRRPAGIRQPDGRGSRPNILTISQRPA
                     EAADRAVPGHWEGDLVFGRSMSPVATLVERSTRFLMLIALPNGHRADTVADALAAKIQ
                     TLPAALTRTLTWDQGHEMAEHARFTVATGIPVYFCDPKSPWQRGSNENTNGLLRQYLP
                     RTVNMRDYTQEDFDTIADELNQRPRQTLDFKTPSEALAEVLR"
BASE COUNT          301 a          521 c          556 g          295 t
ORIGIN      
        1 gcaggcgccg tttcagctgc ccatgatcgg cttcgatccg gttgtggacg tactgctcgc
       61 tgtggtgcca ggcgcctgcg gtcgatgcgc ggactcaaga ccgaccgcgg agccacggtc
      121 gtcatcgccg gacacgcgtt catccaaaac atccgccgcg gacattacga actcggcatt
      181 gagcagccag tgactcggcg ggtcgcggct gccttcagcg agctggcctg gcgatctgac
      241 cgacaagatc aaacgcttac gcttgcctcc ttcgcataat gcaacagcgc cctacgcgct
      301 gttgcattaa aaatcgcgac gggcatgacg cgtggggtga atcctggcgt cgtcggctta
      361 cagggcaata ggaacaattg aacggccgct catgccggcg gtctcaatgg gtcatcgcaa
      421 cactgcgttt cttgactgag aggacgtggt gggtgatggc acggagtccg ttgtcgccgg
      481 cggaggttga cgaggtgtgg gaccggtggc ggtcggggca ggcggtgaag gtgttggcgc
      541 gggcgatgcg tcggcacccg tcgacggtgc gagatctgtt gaagcgatgt ggtggggtgc
      601 gtgcggcgcc tcgccggcgg tgggagctgc gattgtcgct ggccgagcgg gaggagatct
      661 ctcggggctt ggcggcagac cgatcgttgc ggtcgatcgc tgctgggctg gggcgggcgc
      721 cttccaccat ctgtcgtgag gtgtcggcca atggtggccg ggctgagtat cgggcgctgg
      781 aggctgatcg ggccgcgtgg ttgcgagcct cacgaccaaa gccgacgaag ctgtcgcagg
      841 gtccagagtt ggcgcgcgtt gtcaccgaga agttagaact gcgctggtcg ccgcagcaga
      901 tcgcgggctg gctgcgacgc gggacagtcg ggtacgtgtc ccacaagacc atctatcggt
      961 cgctgtttat ccaggcacgt ggcgacctgc ggcgcgagct gaccagatac ctccgtactc
     1021 ggcgggcgat gcgccgcccg gccggaattc ggcagcccga cgggcgtgga tcccgcccga
     1081 acatcctcac catctcccaa cgccccgccg aggccgcaga ccgagcggta cccggccatt
     1141 gggaaggcga cctggtgttc ggtcgcagca tgtccccggt ggccacgctg gtcgagcgtt
     1201 ccacccggtt cctgatgctc atcgcactgc ccaacgggca ccgggccgat acggtcgccg
     1261 atgcgctcgc agcgaagatc cagactctgc cagctgcgtt gacccgaacc ctgacatggg
     1321 atcaaggcca cgagatggcc gagcatgccc ggttcaccgt cgccaccgga atcccggtct
     1381 acttctgcga tcccaaaagc ccatggcagc gcggcagcaa cgaaaacacc aacggcttgc
     1441 tgcgccaata cctaccccgc accgtcaata tgcgcgacta cacccaagaa gacttcgaca
     1501 ccatcgccga cgaactcaac caacgccctc gacaaacgct cgacttcaag acaccatccg
     1561 aagcactagc cgaggtgttg cgatgaccac ctgaacccgc aggcatgtgc ggacgtttgg
     1621 ggcatgccta atacggcagc cgcaccttgt gatgtttgag gcgttgtcgt tgg
//
LOCUS       DATADN010001185         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_131486, whole genome shotgun sequence.
ACCESSION   DATADN010001185 DATADN010000000
VERSION     DATADN010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131486"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..368)
                     /locus_tag="VHH53_15200"
     CDS             complement(126..368)
                     /locus_tag="VHH53_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX2291495.1"
                     /translation="MVTGETGFSDVFYDLVSLQYHALKAGQHYGQYVRDARNRGLDDI
                     ASFFEQVMKEDSERAAKCHQYLAQLSGTQGAGPAAG"
     gene            534..962
                     /locus_tag="VHH53_15205"
     CDS             534..962
                     /locus_tag="VHH53_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291496.1"
                     /translation="MPDKDSPRTAAWLLMGLTGSAPGVLEIVDGRLSYIVHGRGALTG
                     AQLKELERRTGRSGLAEELGNGAVVTLFDVPLEAVGGVVFPWYYFGGGMKLTAAKVGY
                     RFSFLKPQNTQEWPGVADIPEGCAAGKAWRGVLASSVSGT"
     gene            1040..1636
                     /locus_tag="VHH53_15210"
     CDS             1040..1636
                     /locus_tag="VHH53_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291497.1"
                     /translation="MTSPSRTSWIMVTVLVAVLVAGCSTPAPGQERHPLPRLPELGSV
                     VLPGGFLAYDEPTAPYAPQHPIHVDSALFEFGPSDDLGGNHHCRAQITVSPHDPQIAT
                     PSAYTDLLGTKLCDADWFGTQFNESWRLHDPGNPQLWTLQFSYTDVGWFSVSDLQAVG
                     VVLFVPDRRFTVGLWTLEEVYSNDEAVALVQEVARSVG"
     gene            complement(1836..1910)
                     /locus_tag="VHH53_15215"
     tRNA            complement(1836..1910)
                     /locus_tag="VHH53_15215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1876..1878),aa:Thr,seq:tgt)
     gene            2023..>2296
                     /locus_tag="VHH53_15220"
     CDS             2023..>2296
                     /locus_tag="VHH53_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX2291498.1"
                     /translation="MTQGERFTRRVRSALRREAHGLLHAGPPTVQLMRPLGPSVPDDA
                     RVQQVLDLCMRVGEVLLSSGESVDETSQTMVRLAATAGLSAVDVDIT"
BASE COUNT          341 a          782 c          808 g          365 t
ORIGIN      
        1 cgcccatgcc tcaccgggtg cccgcggggg ttcgctatgg gcccaacagg cgcccagcga
       61 ccagccagac gggcaacggc ggcgcgtcga gggcgccggg aagcgcactt cgaacgcgtg
      121 attgctcagc ctgccgcggg accggctccc tgggtgccgg agagctgggc gaggtactgg
      181 tggcacttgg cggcacgctc ggagtcttcc ttcatcacct gctcgaagaa gctggcgatg
      241 tcgtcgaggc cgcggttgcg cgcgtcgcgt acgtactggc cgtagtgctg gccggccttg
      301 agtgcgtggt actgcaggga caccaagtcg tagaacacgt cgctgaagcc ggtctctccg
      361 gtgaccatgt tgcctccaag gcagtcggaa gccgacatcc gcggataccc ggcggcaggc
      421 tgcctcacgc cttcctggag ccgcacctcg gtaagggtcg ccggtcgggg gacgtaacag
      481 tctcgttggg actgccgagt tgagcactgt ctgactgaca gagagggaga agagtgccgg
      541 acaaggattc gccgcggact gctgcttggc tgctcatggg gctgacgggg agcgcaccgg
      601 gtgttctgga gatcgtcgac gggcgcctga gctacatcgt ccacggccga ggggcactga
      661 ccggcgctca gctcaaggag ctcgagcggc gtaccgggcg atccgggctc gccgaagaac
      721 tcggcaacgg ggctgtggtg acgctgttcg acgtgccgct cgaggccgtc ggcggtgtgg
      781 tgtttccctg gtactacttc gggggcggga tgaagctgac agcggccaag gtgggatacc
      841 ggttctcgtt cctcaagccg cagaacacgc aggagtggcc cggagtggcc gacatccccg
      901 agggctgtgc cgccggcaag gcctggcggg gggtgctcgc gtcctccgtg tccggcacct
      961 gaccgcggac gacgatctgc ccaggccgct caccgcttcc ggcgggcggt tctcgggcgc
     1021 caacttcccg gctgctgtcg tgacgtcccc gtcacggacc tcgtggatca tggtgaccgt
     1081 tctggtcgcg gtcctggtcg caggctgctc caccccggca ccgggccagg agcgacaccc
     1141 gctgccacgc ctgccggagc tcggcagcgt cgtgctaccg ggcggcttcc tcgcctacga
     1201 cgagcccacc gcgccgtatg ccccccagca cccgatccat gtcgacagcg ccctgttcga
     1261 gttcggcccc tccgacgacc tgggcggtaa ccaccactgc cgggcgcaga tcaccgtctc
     1321 cccgcacgac ccgcagatcg cgacgccgtc cgcgtacacc gatctactgg gcaccaagct
     1381 gtgcgatgcc gactggttcg gcacgcagtt caacgagtcc tggcgcctgc acgaccccgg
     1441 taatccgcag ctctggaccc tgcagttctc ctacacagac gtggggtggt tctccgtgag
     1501 cgacctccaa gccgtcggcg tggtcctctt cgtgcccgat cgccggttca cggtcgggct
     1561 gtggacgctg gaggaggtct actcgaacga cgaggcagtc gcgctggtcc aggaggtcgc
     1621 gcgctccgtc gggtgagctc cgcgcgtcgg ccataagcgc gcgcatacac gcggacgcgc
     1681 agggcgccgc cgggaagctc atcgatgctc ccccgctggc gccctgacct ggccgttctg
     1741 gccttcacca tgagggatca agccgcggtt gtgccatgaa ctgtgtcaca aagctgaccc
     1801 agggggcgac gccggacaaa cccgctggtc aggcgtggag ccggaggtcg ggatcgaacc
     1861 gacgacctac cgcttacaag gcggttgctc taccactgag ctacaccggc cggtaccgga
     1921 cgccatccta gtagcgcgat cgtggcacgc acctaccctg ggcgccatgc cgcagtcgat
     1981 cgccgccgtc gccgcgggcg gggcccgtcc gggcgggccc ggatgacgca gggcgaacgt
     2041 ttcacccgcc gggtccgcag cgcgctgcgg cgcgaggcgc acgggctgtt gcacgccggc
     2101 ccgcccacgg tgcagctgat gcggccgctg ggcccgagcg tgccggacga cgcgcgggtg
     2161 cagcaggtgc tcgacctgtg catgcgcgtg ggcgaggtgc tgctctcgag cggcgagagc
     2221 gtggacgaga ccagtcagac gatggttcgg ctggccgcca cagctggcct ctcggccgtc
     2281 gacgtcgaca tcacgt
//
LOCUS       DATADN010001186         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_131691, whole genome shotgun sequence.
ACCESSION   DATADN010001186 DATADN010000000
VERSION     DATADN010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131691"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VHH53_15225"
     CDS             complement(<1..545)
                     /locus_tag="VHH53_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291499.1"
                     /translation="MLLRQMNAGSARRIDGRSLTPSQRVVPSWTDPVPRLASRIVGGP
                     LGRHAVVGRHWFWTPLRVVLLLATITLMLGWLAKAPCLQTYPDSAGVLQVDWRDGWQY
                     RAMCYSDTVPLYTAERLDRPGLAGLPYATSWIENRGEPGEQVRYMEYPVITGMYQWVV
                     AKFAQSWLAVADRGWLLGSAPA"
     gene            complement(579..>2748)
                     /locus_tag="VHH53_15230"
     CDS             complement(579..>2748)
                     /locus_tag="VHH53_15230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2291500.1"
                     /translation="NRYDAWYGSRYGGRRNPRPDPGSSRRIRNVALVGLLLAVLAPVL
                     AFFAGWLFLSVPSPATLAAQQKQVTTIMASDGTTEIGRYVPEEGNRIAVDLDQVPVHV
                     QNAVLAAEDRSFRSNPGFDIVGIARATWKQLTGGTGGGSTITQQYVKVATGRDEYSIV
                     RKFREVIVAAKITKQNSKDQILQDYLNTIYFGRGAYGIQAASQAYFGKNVQSLTPSEA
                     AVLAGAIRSPSRLDPAKNLEQSEVRWNFVLDGMVTQGWLTQADRVAARYPATIPPGQA
                     PGSPTDDRAHIVNRVLEELEKHGITREQLAVKGGRISTTIDARAQRLAREAVLIELRD
                     QPRNLYSSLVAIDPRSGGVTAYYGGEEGDGFDLAGGPAWNPGSAFKPFTMLAALKQDI
                     GLNSFYNGDSPLLIDGLSYANSESRDYRSLTLHDAMTESVNTAFVRLAQEVGAQAIRD
                     VAVAAGIPEQINGERAMAELDSGQPAIGITLGQYPVHTIDMASAYATFAADGMRRDPF
                     FVREYLTADGEINYKHVDEPKQAFDPTDIERNSRLARNLTATLIDVASSSKIPLEGGR
                     QVAAKTGTHQLGDTGDNSAAWTVGYTPSISTAVWVGDPANSAIKNGDGSDIFGRGLPG
                     AVWQRFMNTYLAGAPMETFPEFKLIGPAARPAVTPAPPTPAKRERVRTTQSAKPEPDF
                     DSFPIPIEPEEEPRKPSLRDCFPFGCDDSPPEEDDEGEDR"
BASE COUNT          444 a          916 c          913 g          475 t
ORIGIN      
        1 gcaggcgccg aaccgagcag ccagccgcgg tcggccacgg ccagccacga ctgcgcgaac
       61 ttggcgacga cccactggta catgccggtg atcaccgggt actccatata ccgcacctgc
      121 tcgcccggct cgccccgatt ctcgatccac gaggtggcat acggcaggcc tgccaacccg
      181 gggcggtcca gccgctctgc ggtgtagagc gggaccgtgt ccgagtagca catcgcccgg
      241 tactgccagc cgtcgcgcca gtccacctgc agcacgccag cactgtccgg gtaggtctgc
      301 aggcacggtg ctttagccag ccagccgagc atcaacgtga tcgtcgcgag gagcagcacc
      361 acccgtagcg gggtccagaa ccagtgccgg ccaaccacgg cgtgccggcc cagcggtcca
      421 cccacgattc ggctggccaa ccgggggacc ggatcggtcc agctaggcac cacccgctgc
      481 gacggagtca gggagcgccc gtcgatccgc ctggcggagc cggcattcat ctggcgaagg
      541 agcatggcga tgacgttacg aagctaccgg caacgctgtt aacgatcctc tccctcgtca
      601 tcctcctccg gcggggagtc gtcacatccg aacgggaagc agtcccgcaa ggacggcttg
      661 cgcggctcct cctcaggctc gatcgggatg gggaaggagt cgaaatcggg ttcaggcttg
      721 gcggattgcg tggtccgcac gcgctcgcgc ttggccggcg tcggcggagc cggcgtcacc
      781 gcgggccggg ccgccggacc gatcagcttg aactcgggga acgtctccat cggagcgccc
      841 gcaaggtagg tgttcatgaa tcgctgccag accgctcctg gcagacctcg gccgaagatg
      901 tcgctgccgt ctccgttctt gatcgccgag ttggcgggat cacccaccca caccgcggtg
      961 gagatcgagg gggtgtaccc gacggtccag gcggcggagt tgtcgccggt gtcgccgagc
     1021 tggtgggtac cggtcttggc ggcgacctgg cgaccgccct ccaagggaat cttcgagctg
     1081 ctcgcaacgt cgatcaacgt cgcggtgagg ttgcgagcca gccgtgagtt gcgctcgatg
     1141 tccgtcgggt cgaaggcctg tttcggctcg tcgacgtgct tgtagttgat ctcaccgtcg
     1201 gcggtgaggt actcgcggac gaagaatggg tcgcgccgca tcccatcagc ggcgaaggtt
     1261 gcgtaggcgc tggccatgtc gatggtgtgt accgggtact ggcccagggt gataccgatc
     1321 gcgggctgcc cgctgtccag ctcggccatg gcacgctcgc cgttgatctg ctccgggata
     1381 ccggccgcga ccgcgacatc ccggatcgcc tgcgcaccca cctcctgggc cagccgcacg
     1441 aaggcggtgt tgaccgactc ggtcatggcg tcgtgcaggg tcagtgatcg gtagtcgcga
     1501 ctttccgagt tggcatagga caacccgtcg atgagcaggg gagaatcgcc gttgtagaag
     1561 gagttcaggc cgatgtcctg cttgagcgca gccagcatgg tgaagggttt gaaggccgaa
     1621 cccgggttcc acgccggccc accggccagg tcgaagccat ccccttcctc cccgccgtaa
     1681 tacgcggtca cccccccgct tcgcggatcg atcgcgacca gcgaggagta caggttcctc
     1741 ggctggtcac gcagctcgat gagcactgct tcgcgggcca gccgctgtgc ccgagcatcg
     1801 atggtggtgc tgatccgtcc acccttgacg gcgagctgct ctcgagtgat gccgtgcttc
     1861 tccaactcct cgagcacccg gttgacgatg tgcgcccggt cgtcggttgg tgagcccggg
     1921 gcctgccccg gcgggatggt cgccgggtac cgcgccgcga cccgatcggc ctgagtcagc
     1981 cagccctggg tcaccatgcc gtccagcaca aagttccacc gcacctccga ctgctccagg
     2041 ttcttggccg gatccaaccg ggaaggcgat cggatcgcgc cggccagcac cgcagcctcg
     2101 gagggggtca agctctgcac gttcttgccg aagtaggcct ggctggcggc ctggatgccg
     2161 taagccccgc gaccgaagta gatcgtgttg aggtagtcct gcaggatctg gtccttggag
     2221 ttctgcttgg tgatcttggc ggcgacgatc acttcgcgga atttccggac gatgctgtac
     2281 tcgtcgcgtc cggtggcgac cttgacgtac tgctgcgtga tcgtggaccc accgccggtg
     2341 ccaccggtga gttgcttcca cgttgcccgc gcgattccga cgatgtcgaa gccggggttg
     2401 gatcgaaagc ttcgatcctc cgcggccagc acggcgttct gcacgtgcac cggaacctgg
     2461 tccagatcga ccgcgattcg attgccctcc tcaggcacgt agcggccgat ctcggtggtc
     2521 ccgtcactgg ccatgatcgt ggtgacctgc ttttgctgcg ccgccagcgt cgcgggcgac
     2581 ggcacgctga ggaacaacca gccggcgaag aacgccagca ccggcgccag gaccgcgaga
     2641 agcaggccca caagagcaac gttacggatc cgccgggaac ttcccgggtc cgggcgcggg
     2701 ttgcggcgcc cgccatatcg actgccatac caagcgtcat acctgtta
//
LOCUS       DATADN010001187         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_750046, whole genome shotgun sequence.
ACCESSION   DATADN010001187 DATADN010000000
VERSION     DATADN010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750046"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..692
                     /locus_tag="VHH53_15235"
     CDS             207..692
                     /locus_tag="VHH53_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291501.1"
                     /translation="MNTPQEHHEQYERSQAPGARVRRFLDDEQPVGVQTGHPGSPGLD
                     HPGGAAQQEPVASSGAPAHVEQPVAAAAEDTARDGGAQHVGLINDVAGLRDEWQRVQG
                     TFVDDPQRAVHEASVLIDRTLDEIRMNVGSGHPSETASTEDLRVRFQRYREFFQRLLS
                     A"
     gene            918..1184
                     /locus_tag="VHH53_15240"
     CDS             918..1184
                     /locus_tag="VHH53_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291502.1"
                     /translation="MNMVIILEILVGIVVVAAAVRFAVRDARQRRGVAEEQHTSEVLA
                     HGGERQAEQAPSDAVEQREGDEVAALSAPATAPAIGPEPPADTR"
     gene            1194..2117
                     /locus_tag="VHH53_15245"
     CDS             1194..2117
                     /locus_tag="VHH53_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX2291503.1"
                     /translation="MLRALILAAAGNPTVRRLVATAPVSRYVVRRFVAGESTQQALTA
                     ASALVANGLAVTLDHLGEGIEDVAGAESTVHAYLELLERVKAGGLADRVEVSVKLSAV
                     GQRLDEALALRNASRICAAAERAGTTVTLDAEDHTLTESTLRVHAQLRRSWPATGAVL
                     QAYLYRTLDDCRALAVEGARVRLCKGAYAEPPELAFATSHEVARNFVRCANTLLAGPG
                     YPMFATHDRRLVAIVGERARWYGRPPGSFEYQMLYGVRPAEQQRLAAGGDTVRVYLPY
                     GSDWYSYLTRRLAERPENAAFFLRALLRSGD"
     gene            2194..>2549
                     /locus_tag="VHH53_15250"
     CDS             2194..>2549
                     /locus_tag="VHH53_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2291504.1"
                     /translation="MTTIAILGAGKIGEALLSGMLRAGRSPDNLMLTERYPERAAQLE
                     ETYRVRAVDTATAARKADVLVVAVKPQDIDPLLAELAGLIDPSTLVVSLCAGLPTTLY
                     ERRLPDGVPVVRVMPNT"
BASE COUNT          419 a          862 c          869 g          399 t
ORIGIN      
        1 cacccgcagg ttgtcgagca ctaccgggct gcgcatgccg cccgtcaagc ccatcacggt
       61 gaggccggct cgtcggacac cgaggaactg cggcaggcgt tcgttcacta ccgggcgctg
      121 ttcgaggagc tcgtgcatgg ccgtcaagac acgtcgagaa agccgaacac gcgataaccc
      181 gagataacac ggccggaggc ttctggatga acacgccgca ggaacaccac gagcaatacg
      241 agcggtcgca ggctccgggt gcgcgggtgc gtcgcttcct cgacgatgag cagccagtag
      301 gggtgcagac cggtcatccg ggtagtccgg gtctggacca ccccggcggc gccgcgcagc
      361 aagagccggt cgcctccagc ggcgcgcctg cccacgtcga gcagccggtg gcggccgctg
      421 ccgaagacac tgcccgcgat ggtggcgcgc agcacgtggg gttgatcaac gatgtggccg
      481 ggctacgtga cgaatggcag cgcgtgcagg gaacattcgt cgacgatccg cagcgcgcgg
      541 tacacgaggc cagcgtgcta atcgatcgga cgctggacga gatccgcatg aacgtcggaa
      601 gtgggcaccc gagcgagacg gcgtcgaccg aagatttgcg ggtgcgcttc cagcgttatc
      661 gagagttctt ccagcgcctg ctgtctgcat gatctcaacc cagcacaggt agcgcgccgc
      721 ctgcactcct gctgcgcgca ctggtcctgc cttgctgccg gcaatccggc cgtcggtgat
      781 cgacgcccta cacgacatgc gcttatgtct gtgagcgacc cgacaacgtg acaataggct
      841 gaatgggcgt atcgttgcgc tgctccgcaa cggggtaggt cgctactggt gaatcacaag
      901 gagtgtgaaa gcttgtcatg aacatggtca tcatcctcga gatactcgtc ggtatcgtcg
      961 tcgtcgcggc ggccgtgcgg ttcgcggtgc gggacgctcg gcaacgtcgc ggtgtcgccg
     1021 aagagcagca caccagcgag gtgctggcgc atggcggtga gcgccaggca gagcaggccc
     1081 cgagcgacgc cgtcgaacag cgtgagggcg acgaggtcgc cgcgctgagc gccccggcga
     1141 ctgcccccgc gatcggcccg gagccgcccg cagacacgcg ctagcgtgcg tccgtgctgc
     1201 gcgcactgat cctggccgcc gccggcaacc cgaccgtccg gcggttggtg gccaccgcgc
     1261 cggtcagccg gtacgtcgtg cgccgatttg tggccgggga aagcacacag caggcgctca
     1321 ctgcggccag cgcgctggtg gccaacggtc tcgccgtgac cctggaccac ctgggggagg
     1381 gcatcgaaga tgtggccggc gcagaatcta ccgtccacgc ctacctcgag ctgctggagc
     1441 gggtgaaagc cggtggtttg gccgaccggg tcgaggtgag cgttaagctc tccgctgtcg
     1501 gccagcgact tgacgaggca ctggcattgc gcaacgcctc ccggatctgc gctgcggccg
     1561 agcgcgccgg cacgaccgtc accttggacg ctgaggacca cactctcacc gagtccaccc
     1621 tgcgcgtgca cgcgcaactg cgccgcagct ggccggctac cggagcggta ctgcaggctt
     1681 acctgtaccg gacgctggat gactgccggg cgctggcagt cgagggcgct cgggtccggc
     1741 tgtgtaaggg cgcctacgcc gaaccgcctg agctggcatt cgcgacgtcg cacgaagtcg
     1801 cgcgcaactt cgtgcgctgc gccaataccc tgctggccgg accgggctac ccgatgttcg
     1861 ccacccacga tcgccggctg gttgccatcg tgggtgagcg agcccgctgg tatggccgcc
     1921 caccgggcag cttcgagtac cagatgctct acggagtgcg tccggccgag caacaacggc
     1981 tggccgcagg cggcgacacc gtccgggtgt acctgcccta cggctcagac tggtactcct
     2041 atctcacccg acggctcgcc gagcggcccg agaacgccgc cttcttcctg cgcgccttgc
     2101 tgcgttctgg ggactgagtg cagtgaacgg gggtccgctt actgcactca gtccccagaa
     2161 cgcccgtgta cggagctgtg gcaccctgcg gccatgacca cgatcgccat cctgggtgcc
     2221 ggcaagatcg gcgaggcctt gctgtccgga atgctccgcg ccggccgcag tcctgacaac
     2281 ctgatgctca ccgagcgcta cccggaacgg gctgcgcagc tcgaggagac ctaccgggta
     2341 cgcgcggtcg acactgccac cgccgcacgt aaggccgacg tcctggtcgt tgcggtgaag
     2401 ccgcaggaca tcgacccgct gctggccgag ctcgccgggt tgatcgaccc gagcaccctg
     2461 gtcgtgagct tgtgcgcggg tctgcccacc acgctctacg agcgtcggct tccggacggc
     2521 gtgccggtcg tccgggtcat gcccaacac
//
LOCUS       DATADN010001188         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_132466, whole genome shotgun sequence.
ACCESSION   DATADN010001188 DATADN010000000
VERSION     DATADN010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132466"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VHH53_15255"
     CDS             complement(<1..326)
                     /locus_tag="VHH53_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX2291505.1"
                     /translation="MSSSVRGDGTRSDEAEWAALGDVEGIDSEPDVALEQPVLAVVGR
                     PNVGKSTLVNRILGRREAVVQDVPGVTRDRVAYDALWNGRRFTVVDTGGWQPDARGLP
                     GHGGLQA"
     gene            complement(326..997)
                     /locus_tag="VHH53_15260"
     CDS             complement(326..997)
                     /locus_tag="VHH53_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2291506.1"
                     /translation="MIDDVPEGTWLWLRDVTRWIGTWCFFVGYRLEVRHRDRMPASGP
                     IVVVANHSAIVDGPLLYGVLGRPTSFLIKQEIFRGPLGWILPRLGQLAVRRGAADRAP
                     LLTAIRLLRHGGVVVVFPEGTRGEGDVDNAHQGAVWLARTGGARLLPVAIRGTRRPPR
                     SNWRFRPRVDVLVGEPFAVPPGRGRAALVAATNEMRDRLVTLVAELDRIRGVGMPRAI
                     RPKGA"
     gene            complement(990..1739)
                     /gene="cmk"
                     /locus_tag="VHH53_15265"
     CDS             complement(990..1739)
                     /gene="cmk"
                     /locus_tag="VHH53_15265"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX2291507.1"
                     /translation="MEPSASREVSRVQQGDGLRGVVAVDGPAGTGKSTVARRLAASLG
                     ARYLDTGAMYRAAALGVLRAGADPCEPDAVYEVVARRHIEVGTDPDHPRVLLDGEDVA
                     GEIRGPAVTTAVSPVSAMPEVRRLLVEQQRRIISDASAGGGIVVEGRDIGTVVVPDAA
                     LKVFLTADAAQRAARRAAQDGVEDVAEIHRDVERRDRFDSSRATSPLCAAEDAVEVDT
                     TALDVDAVVGRLQELVIDRGLVAMVVWGRSD"
     gene            complement(1772..>1965)
                     /locus_tag="VHH53_15270"
     CDS             complement(1772..>1965)
                     /locus_tag="VHH53_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX2291508.1"
                     /translation="LVEMVLHEGRKHVVRRLLAEVGHPVQRLVRTTIADVRLGEQRPG
                     TVRVLSRPEVGALNRAVGR"
BASE COUNT          307 a          792 c          609 g          257 t
ORIGIN      
        1 gcctgcaggc cgccgtggcc gggcaggcca cgcgcgtcgg gctgccaccc accggtgtcc
       61 accacggtga accggcgccc attccacagc gcgtcgtagg ccacccgatc cctggtcacc
      121 ccgggcacgt cctgcacgac cgcctcacgg cggcccagga tccggttgac cagcgtcgac
      181 ttacccacgt tggggcggcc caccaccgcg agcacgggtt gttcgagtgc cacatccggc
      241 tcggagtcga tgccctcgac gtctcctagg gctgcccact cggcttcgtc agaccgagtc
      301 ccatcaccgc gcacagaact gctcatcacg ccccctttgg ccgtatcgcg cgcggcatgc
      361 cgacaccgcg gatccggtcc agctcagcaa ccagcgttac cagccggtct cgcatctcgt
      421 tggtcgcggc gaccagtgcc gctcgccccc ggcctggggg gaccgcgaag ggctcgccga
      481 ccaggacgtc gacccgcggc cggaaccgcc agttgctccg aggcggccgc cgggtcccgc
      541 ggatcgccac cggcagcagt ctcgccccac cggtccgggc cagccacacg gcgccctggt
      601 gggcgttgtc cacgtcgccc tcgcctcggg tgccctccgg gaataccacc acgacaccgc
      661 cgtgccgcag cagccggatg gcagtgagca acggcgcacg gtcggccgca ccgcggcgca
      721 cggctagctg gccgagccgc ggcagtatcc agcccagcgg gccgcggaat atctcctgct
      781 tgatcagaaa cgaggtgggc cgtcccagca ccccgtacag cagcgggccg tcgactatcg
      841 cgctgtggtt ggccaccacc acgatgggtc cgctggccgg cattcgatcc cggtggcgca
      901 cctctagccg gtaaccgacg aaaaagcacc aggtcccgat ccagcgcgtc acgtctcgca
      961 gccacagcca ggtgccctca gggacgtcgt caatcactgc gtccccaaac caccatggcg
     1021 accaacccgc ggtcgatcac cagctcctgg agccggccca cgacggcgtc gacgtccagc
     1081 gcggtggtgt ccacctccac agcatcctcg gcggcacaca gcggcgacgt tgcccggctg
     1141 gagtcgaacc ggtcgcggcg ctcgacgtcg cggtggatct ccgccacatc ctcgacccca
     1201 tcctgggctg cccgccgcgc ggcccgctgc gccgcgtccg cggtgagaaa gaccttcagc
     1261 gcagcgtcgg gcaccaccac ggtgccgatg tcacgcccct cgacgacgat cccgccaccg
     1321 gcagaggcgt cggagatgat ccggcgctgc tgctcgacga gcaggcgacg tacctcgggc
     1381 atcgccgaga ccggactgac agcggtggtc accgccggac cccggatctc ccccgcgacg
     1441 tcctcgccat ccagcagcac ccgcgggtgg tccgggtccg tacccacctc gatgtgccgc
     1501 cgtgccacca cctcgtacac ggcgtccggc tcgcaagggt cggcaccagc tcgcaacaca
     1561 cccagcgcgg cggctcgata catcgcaccg gtgtccaaat accgggcgcc cagcgacgcc
     1621 gccagccggc gagccacggt ggacttcccg gtaccggcag gcccgtccac tgccaccacg
     1681 ccgcgcaatc cgtcgccttg ctgcacccga ctgacctccc ggctcgctga cggctccatt
     1741 ctgcccgcca caaccaatgg cggctccagc gctaccggcc gaccgcgcgg ttgagggcgc
     1801 caacctcggg gcgcgacagc acccgaaccg tcccaggacg ctgctcgccg agacggacgt
     1861 ccgcgatcgt ggtacgtacc aggcgctgca ccggatggcc cacctcggcc agcaaccggc
     1921 gcacgacgtg cttgcggccc tcgtgcagca ccatctcgac cagtg
//
LOCUS       DATADN010001189         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_750456, whole genome shotgun sequence.
ACCESSION   DATADN010001189 DATADN010000000
VERSION     DATADN010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750456"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1118)
                     /locus_tag="VHH53_15275"
     CDS             complement(123..1118)
                     /locus_tag="VHH53_15275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2291509.1"
                     /translation="MNSTRKLLAPLYGCRSWLLQQQWFTSRLLPALPRSLRWTLRKLY
                     FLPSDLIERVLSDREELVPPRSSIFTGSVDDFKSSGEALVRRLAHSGCLTPESKVLDI
                     GSGMGRVAVALISYRDGSGSYQGLDIVPSGIKWCSENITSKYPNYTFTLADIFNKEYN
                     PGGRLKASEYRFPFDDETFDLAVLASVFTHMLPEDMRHYIAEVARVLKRGGTCCASYN
                     LLDAESLRTMKAGRGAFRFTRLGPHWVVDVNVPELAVAYEEDYVRKLFEEYGLSCTIH
                     HGWWSGRTDTSRQAPWFLDQDDPDFRYFDQDYVLATKAERYERVAAAARAGTPRA"
     gene            complement(1132..>2058)
                     /locus_tag="VHH53_15280"
     CDS             complement(1132..>2058)
                     /locus_tag="VHH53_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291510.1"
                     /translation="VTVVACAASLVGLLLALPSAIGAGRSGPKMAVATTAAPTTLPQT
                     SGTLRASTTKGARPRTAIGAPASTRTPTTTTSGGRPSAPAAAAGWVNVVNDQFSSGGV
                     PAHWHRYDGPYGSEPRNCATPSHVSVSGGSMHLLMRHETSGRCGAGWYTAGMMLDRAF
                     ASVDQRVTVRFRVVSGGVSGHHIIPMRFPPGAPWPQGGEEDYCEGDGSRGCYTFLHYG
                     DKASTQVLHRHAFDLSQWHTVRFERRNHVVKAYIDDLTTPVWTYNGSSRTLPDTRKQV
                     VLQQECQAPGCPAGTTGTEDIQIDWITVDNPV"
BASE COUNT          337 a          665 c          703 g          353 t
ORIGIN      
        1 cgggtcagtc ggccagcgga aataagccca ggctgccgtg acgtatggat ctatgccgtg
       61 cgaggaccaa gatctggatg tcctccgtgc cgctggtcca gggccgaatg ccctgtcgac
      121 catcaggccc gcggtgtacc agcccgcgcc gcggcggcca ctctttcata ccgctctgcc
      181 ttggtcgcga gcacatagtc ctgatcgaag taccggaagt cagggtcgtc ctggtcaagg
      241 aaccaaggcg cttggcgcga tgtatccgta cgacccgacc accagccgtg gtggatcgtg
      301 cacgacagcc cgtattcctc gaacagcttc cggacgtagt cctcttcgta ggcgacggcc
      361 aactccggga cgttgacgtc gaccacccag tgcgggccga ggcgtgtgaa gcggaacgca
      421 ccccgacctg ccttcatggt gcggagcgat tccgcgtcga gaaggttgta gctggcacag
      481 caggtgccgc cccgcttgag gacccgggcg acctcggcga tgtagtgccg catgtcctca
      541 gggagcatgt gggtgaatac ggatgcgagc accgcgaggt cgaacgtctc gtcatcgaag
      601 ggaaagcggt actcggaggc cttgagccgc ccgccggggt tgtactcctt gttgaagatg
      661 tcagccagcg tgaaggtata gttcgggtac ttggaggtga tgttctcgct gcaccacttg
      721 atccctgacg ggacgatgtc gagaccctgg taggacccgc ttccgtctcg gtaggagatc
      781 aatgccacgg cgacgcgccc catcccagaa ccgatgtcca ggaccttcga ttccggcgtc
      841 agacacccag aatgggcaag ccgacggaca agggcctcgc cgctgctctt aaaatcgtcg
      901 accgagcccg tgaagatcga tgacctggga gggaccaact cctcccgatc gctgagcact
      961 cgctcgatca ggtccgacgg caggaaatag agcttgcgca gcgtccatcg cagcgaccgc
     1021 ggcagcgccg gcaagagccg gctggtgaac cactgttgct gcaggagcca gctccggcag
     1081 ccgtatagag gcgccagcag ctttcgggtc gagttcacca cattcccttt ctcagaccgg
     1141 gttgtcgacg gtgatccagt cgatctggat gtcctcggtg cccgtggtcc cggccggaca
     1201 tcctggggcc tggcactcct gctgcagcac cacctgcttc ctggtgtcgg gcagcgtcct
     1261 ggaggagccg ttgtaggtcc agaccggcgt ggtcaggtcg tcgatgtagg ccttgaccac
     1321 gtggttgcgc cgctcgaacc gcaccgtgtg ccactgcgaa aggtcgaagg cgtggcgatg
     1381 caagacctgt gtgctggcct tgtccccgta gtgcaggaag gtgtagcagc ccctggatcc
     1441 gtcgccctcg caatagtcct cctcgccgcc ctggggccag ggggcacccg gcgggaagcg
     1501 catggggatg atgtgatggc cgctgacccc gccgctgacc acgcggaacc gcacggtcac
     1561 tcgctggtcg acgctggcga aggccctgtc gagcatcatg ccggcggtgt accagccggc
     1621 gccgcaccgg ccgctcgtct cgtgccgcat cagcaagtgc atcgagccgc cggacaccga
     1681 tacgtgcgag ggggtggcgc agttgcgcgg ttcggagccg tagggcccgt cgtaccggtg
     1741 ccagtgtgcc ggcaccccac cggagctgaa ctggtcgttg accacgttga cccagcccgc
     1801 cgccgccgcg ggcgcggatg gacgcccgcc gctggtcgtc gtggtggggg tgcgggtgga
     1861 ggccggggcg ccgatggccg tccgtgggcg cgctcccttg gtggttgaag cccgcaaggt
     1921 gcccgacgtc tgcggcaggg tcgtgggggc cgccgttgtc gcgacggcca tcttcggtcc
     1981 ggaccgcccc gctccgatcg cgctcgggag ggccagcagg aggccgacca ggctcgctgc
     2041 gcaggcgacg acggtcac
//
LOCUS       DATADN010001190         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1342574, whole genome shotgun sequence.
ACCESSION   DATADN010001190 DATADN010000000
VERSION     DATADN010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342574"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /gene="hisF"
                     /locus_tag="VHH53_15285"
     CDS             complement(<1..658)
                     /gene="hisF"
                     /locus_tag="VHH53_15285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX2291511.1"
                     /translation="MAVAVRVIPCLDVDAGRVVKGVNFTALRDAGDPVELATAYDAQG
                     ADELTFLDVTASSADRETTYDVVRRTAECVFIPLTVGGGIREVSDVDRLLRAGADKVS
                     INTAAVARPELLREASHRFGAQCIVLSVDARRVPPGEPATPSGFEVTTHGGRRGTGID
                     AVAWAARGQELGVGEILLNSMDADGTKAGFDLEMISAVRAVVTVPVIASGGAGDVGHF
                     P"
     gene            complement(735..1466)
                     /gene="priA"
                     /locus_tag="VHH53_15290"
     CDS             complement(735..1466)
                     /gene="priA"
                     /locus_tag="VHH53_15290"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HEX2291512.1"
                     /translation="MSFVLLPAVDVAGGRTVRLTQGEAGTETCYGDPVESALAWQADG
                     AEWIHLVDLDAAFGRGSNAELLAEVVGRLDIAVELSGGIRDDASLQRALATGCARVNL
                     GTAALEDPAWCRSALAEHGERIAVGLDVRIIDGQHILAARGWTARGGDLWEVLERLDA
                     DGCPRYVVTDVTKDGTLRGPNLDLLRAVCAQTEAPVIASGGISGAADVAALAALTPKG
                     VEGAIIGKALYTGALTLPDALAAAR"
     gene            complement(1485..1670)
                     /locus_tag="VHH53_15295"
     CDS             complement(1485..1670)
                     /locus_tag="VHH53_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291513.1"
                     /translation="MPAGNAGCVRAVALLAVVAMVASVGYSALAVLQAPTWLVLALVA
                     VLAGLLYWLLGSNEERS"
     gene            complement(1689..>1816)
                     /locus_tag="VHH53_15300"
     CDS             complement(1689..>1816)
                     /locus_tag="VHH53_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX2291514.1"
                     /translation="AEHGEPFVAAVENGPLWATQFHPEKSGDAGSHLLRNWIEIL"
BASE COUNT          314 a          680 c          576 g          246 t
ORIGIN      
        1 gcgggaaatg ccccacgtcg cctgcgccgc cgctggcgat caccgggacc gtgacgacgg
       61 ctcgcaccgc ggagatcatc tcaagatcga agcccgcctt ggtgccgtcg gcgtccatcg
      121 aattgagcag gatctctccc actccgagct cctggccgcg ggccgcccag gccacagcat
      181 cgatcccggt accacgccgc ccgccgtggg tggtcacctc aaagccggat ggcgttgctg
      241 gctcgccggg cggcacccgg cgagcatcca ccgacagcac gatgcactgc gcaccgaagc
      301 gatggctggc ctcacgcaac agctcgggac gggccaccgc ggcagtattg atgctcacct
      361 tgtctgcgcc agcccgcagc aagcggtcca catcggacac ttcccgaatt cctccgccga
      421 cggtgagcgg gataaagacg cattccgcgg tgcggcggac cacgtcatag gtcgtctccc
      481 ggtccgccga ggacgcggtg acgtcgagga aggtcagctc gtcggcgccc tgcgcgtcat
      541 aagcggtagc caactcgacc ggatcaccgg cgtcgcgcag tgcggtgaag ttcacccctt
      601 tgaccacccg gccggcatcg acatccaggc acgggatcac acgcacggca acagccattc
      661 gtccagccta agcgggcgag cacggcgtgc ggacacaccg ggcaacagag caagacacgc
      721 gcggcggatg ggggtcagcg cgcggcggcc agcgcgtcgg gcagggtgag cgccccggtg
      781 tacagcgcct taccgatgat tgcgccttcc acgcccttgg gggtcagcgc ggccaatgcg
      841 gccacgtcgg cagctccgga gatcccaccg gaggcgatca ccggcgcttc ggtttgggcg
      901 cagacagcac gcagcaggtc caggttcggt ccgcgcagag tgccgtcttt ggtcacatca
      961 gtgaccacgt agcgcgggca gccgtcggcg tccaggcgtt ccaggacctc ccagaggtcg
     1021 ccgccccgag cagtccagcc gcgtgcggcg aggatgtgtt gcccgtcgat gatgcgcaca
     1081 tcgagcccca ccgcgatccg ctcaccgtgc tcggcgagcg ccgaccggca ccaggccgga
     1141 tcctccagcg cggcggtgcc caggttcacc cgggcgcatc cggtggccag cgcgcgctgc
     1201 agcgacgcgt catcccggat cccgccggag agctccaccg cgatgtccaa cctgcccacc
     1261 acctcggcca gcagctcggc gttggagccc cgaccgaacg ccgcgtccaa atcgaccagg
     1321 tgaatccact cggcaccatc ggcctgccag gccagcgcgg actcaaccgg gtcgccgtaa
     1381 caagtctccg tgccggcttc gccctgggtg aggcggacgg tccggccgcc ggcgacgtcc
     1441 accgcgggca gcagcacgaa actcaccggc ttaccctaaa ccgctcacga tcgttcctcg
     1501 ttactgccca gcagccagta cagcaagccc gccagcactg ctacgagcgc caggaccagc
     1561 caggtcggtg cctgcagcac cgccagcgcc gagtacccca ccgaggccac catcgccacc
     1621 accgcgagca gggccaccgc ccgcacgcag cccgcgttgc ctgccggcat gccgtatctg
     1681 cccatcgact acaagatctc gatccagttg cgtagcaggt gagaaccggc gtcaccggat
     1741 ttttcgggat ggaactgggt ggcccacagc ggcccgttct ccaccgcagc gacaaacggc
     1801 tcgccgtgct cggccc
//
LOCUS       DATADN010001191         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_750663, whole genome shotgun sequence.
ACCESSION   DATADN010001191 DATADN010000000
VERSION     DATADN010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750663"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..760)
                     /locus_tag="VHH53_15305"
     CDS             complement(158..760)
                     /locus_tag="VHH53_15305"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX2291515.1"
                     /translation="MRAAEAVDVTPFVRVPEADPAAVLRCLDAGAMGVVIAHVRRRAD
                     VNAAIRAARYAPGGMRSLGGGRVPGFGRIDLVEYVRMANAEIMAVPMIEDEHAVADLP
                     AILAGGSVDLILEGAADVSQAYGVPCQTRHPTMRGAVRRIHAVCAQAGVPFCAIPRTR
                     DDHAQWRAAGVRAFVPGEERGLAARTLRAHLADHRSPDNL"
     gene            complement(774..>1591)
                     /locus_tag="VHH53_15310"
     CDS             complement(774..>1591)
                     /locus_tag="VHH53_15310"
                     /inference="COORDINATES: protein motif:HMM:NF018031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IucA/IucC family C-terminal-domain containing
                     protein"
                     /protein_id="HEX2291516.1"
                     /translation="PMEHAQRAADAGRLLTALRREWAYDGFEVLLETGYRTIDDTMLA
                     ADFTVLYRENPFATREHAPQVVAGLLEDNADGTEPELLRYVRQATARPQGQLPVDHVA
                     GWLRRYLEISLLPLLAVFSAHGVSLEAHVQNSLLHLDGGWPARFFVRDMEGTSVSRQR
                     CDAGSRHVLPADSPVLYDDAETWLRLKYYVVTNHLGHLVHVLGRHAGVDEWRLWQVVR
                     QLLSDRCRADRYAADLLCSPMLPAKANLIGRFAGWGKRPLYVDIPNPMYEVSR"
BASE COUNT          257 a          555 c          526 g          253 t
ORIGIN      
        1 ctgttggcct tcatggcata gaacatccgg catcggttgg gcagcgcgct gaccacccgg
       61 ctgacgtggt cggtcaactg gtccaggtca tacccgtata ggcacaccgg ttcgctgccg
      121 ccgagacggt tcaggatcag cgtgctgatg gcgtcgctca taggttgtcc ggtgatcggt
      181 gatccgcaag gtgtgcccgc agggtgcgag cggccagtcc ccgttcctct ccgggcacga
      241 aagctcgcac cccggcagcg cgccactgcg cgtggtcgtc ccgagtacgg ggaatcgcac
      301 agaagggcac accggcctgc gcgcacaccg cgtgaatccg gcgcaccgcc ccgcgcatgg
      361 tggggtgccg ggtctggcag ggcacgccgt aggcctggga cacgtccgcg gcgccttcga
      421 ggatgagatc cacactgccg cccgccagga tggcaggcag gtcggccact gcgtgctcgt
      481 cctcgatcat cggcacggcc atgatctccg cattggccat ccgcacgtac tcgaccagat
      541 cgatccgacc gaacccgggc actcgtccac ctcccaggct gcgcattccc cccggggcgt
      601 accgggcggc ccggattgcg gcgttcacgt ccgctcgccg ccgaacgtgc gcaatgacga
      661 cacccatagc gcctgcgtcc aggcagcgca acacggctgc ggggtcggcc tcgggtaccc
      721 gcacgaacgg ggtgacatca accgcctcgg ccgcccggat aaggttcttc cggtcatcta
      781 ctcacctcgt acatcggatt cgggatgtcc acataaagtg gtcgctttcc ccatccggcg
      841 aagcggccga tcaggttggc cttggctggc agcatcgggg agcacagcaa gtcggccgca
      901 taccggtcgg ctcgacaccg gtcggacagc agctgccgca ccacttgcca caaccgccac
      961 tcgtcgaccc ccgcgtgccg gcccaggacg tgcacgaggt gaccgaggtg attggtgacg
     1021 acgtaatact tcagacgcag ccacgtctcg gcgtcgtcgt agagcaccgg gctgtcggcg
     1081 ggcaacacgt ggcggctacc cgcatcgcag cgctgccggc tcacgctggt gccctccatg
     1141 tcgcgcacga agaaccgggc cggccagccg ccatcgaggt gcagcaacga gttctgcacg
     1201 tgggcctcga ggctcacccc gtgtgcggag aacaccgcga gcagcggcag cagggagatt
     1261 tccaggtagc gacgcaacca cccggcgacg tgatcgaccg gcagctgtcc ctgcgggcgc
     1321 gcggtggctt gccggacgta ccggagcagc tccggttccg tcccgtcggc gttgtcctcc
     1381 agcaggccgg cgacgacctg aggtgcgtgc tcgcgagtgg cgaacgggtt ttcccggtac
     1441 agcacggtga agtcggcggc cagcatggtg tcgtcgatcg tgcgatatcc ggtctccagc
     1501 aacacctcga atccgtcgta ggcccactcc ctgcgcaggg ccgtgagcag ccggccggcg
     1561 tcggcggccc gctgtgcatg ttccatcggg t
//
LOCUS       DATADN010001192         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_891074, whole genome shotgun sequence.
ACCESSION   DATADN010001192 DATADN010000000
VERSION     DATADN010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891074"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VHH53_15315"
     CDS             complement(<1..1392)
                     /locus_tag="VHH53_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX2291517.1"
                     /translation="MFRTLRRALCVAAALLLPLAGTQSSAHAAVGAQAGAGYWHTSGR
                     QILDAAGQPVRIAGVNWFGFETSNHVVHGLWARDYKSMIDQMKSLGYNTIRMPYSDDI
                     LKPGTMPDSISHDGKNTDLRGLTSLQVLDRIVAYAGQAGLKIVLDRHRPDAAGQSALW
                     YTASVPESTWIANLKSLAARYQGNSTVIGIDLHNEPRDPACWGCGDTARDWRLAAQRA
                     GNAVLSVNPELLIMVEGVQSYNGANGWWGGNLMGVAQHPVQLDVPDRLVYSAHDYATS
                     VAQQPWFGDPSFPANMPEVWDRYWGYVFKQNIAPVWLGEFGTTLQPAVDQKWLSALVT
                     YLRSTSAYGADSFHWTFWSWNPNSGDTGGILKDDWQTVDTAKDGYLEAIKAPGFEGGG
                     SGPDPGGPGDPGGTPACTAAYAVSSDWGGGFNAEVKVTNTGSTPLKSWKVTWAWSGAQ
                     KVTSMWNASYTQSG"
     gene            complement(1534..>2063)
                     /locus_tag="VHH53_15320"
     CDS             complement(1534..>2063)
                     /locus_tag="VHH53_15320"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2291518.1"
                     /translation="STKGVIDRFRSMPMASSAVVAGRGVTDLLVACAELAIMMLTALA
                     MGWRPDGGLGFLAAFGLLLWLRFALIWIGVWLGLLVPNPEAAGGLFAVAFPLTMISSI
                     FVAPQLMPDWLGWVAMWNPISSTAAATRELFGTPVGGDTWVEQHALLMAGVWPVILTL
                     VFLPLAVRRFKKLSR"
BASE COUNT          321 a          725 c          718 g          299 t
ORIGIN      
        1 cccgctctgc gtgtacgacg cgttccacat gctggtgacc ttctgcgcac cgctccacgc
       61 ccacgtgacc ttccaggact tcagcggcgt actgcccgtg ttggtcacct tcacctcggc
      121 gttgaagccg ccgccccagt cgctgctcac cgcgtacgcg gcggtgcagg caggcgtgcc
      181 acccgggtcg cccggacctc cgggatccgg cccggaaccg cccccctcga accccggcgc
      241 cttgatcgcc tcgaggtacc cgtccttcgc ggtgtccacc gtctgccagt cgtccttgag
      301 gatgcccccg gtgtcacccg agttggggtt ccaggaccag aacgtccagt ggaagctgtc
      361 ggcgccgtac gccgacgtcg accgcaggta cgtcaccagc gcggacagcc acttctggtc
      421 caccgccggc tggagcgtcg taccgaactc gccgagccac accggcgcga tgttctgctt
      481 gaagacgtaa ccccagtacc ggtcccagac ctccggcatg ttggcgggga aggacgggtc
      541 gccgaaccac ggctgctggg ccaccgacgt ggcgtagtcg tgggccgagt agaccagccg
      601 gtccgggacg tccagctgga ccgggtgctg ggcgacgccc atgaggttgc cgccccacca
      661 gccgttggcg ccgttgtacg actgcacgcc ctcgaccatg atcagcagct cggggttgac
      721 cgacaggacc gcgttgcccg cgcgctgggc ggccaggcgc cagtcccggg ccgtgtcgcc
      781 gcagccccag caggcggggt cgcggggctc gttgtgcagg tcgatgccga tcacggtgga
      841 gttgccctgg tagcgggccg ccagggactt gaggttggcg atccaggtcg actcggggac
      901 cgacgccgtg taccagagcg ccgactggcc cgccgcgtcc gggcggtgcc ggtcgaggac
      961 gatcttgagg ccggcctgac cggcgtacgc cacgatccgg tccaggacct gcaaggacgt
     1021 cagcccgcgc aggtcggtgt tcttgccgtc gtggctgatg ctgtcgggca tggtgccggg
     1081 cttgaggatg tcgtcgctgt agggcatccg gatggtgttg tagcccagcg acttcatctg
     1141 gtcgatcatg ctcttgtagt cgcgggccca caggccgtgg acgacgtggt tcgaggtctc
     1201 gaagccgaac cagttgacgc cggcgatacg gaccggctgc ccggccgcgt ccaggatctg
     1261 ccggccgctg gtgtgccagt agccggcgcc ggcctgcgcg ccgacggcgg cgtgtgccga
     1321 cgactgcgta ccggccaggg gtaacaggag cgccgcggcg acgcacagcg ctctacgcag
     1381 agtgcggaac atggctgctt cctctcgggg gaggcgcggc cccggaacat ggatgggagc
     1441 gctcccatgt gcgccgcgca cctcccagga aactgacgct gcatgagcac gtcaagcctc
     1501 tgggaggtgc gcgttccgct cggacgacag ggcctaacgg ctcagcttct tgaaccgccg
     1561 caccgccagc ggcaggaaga cgagcgtcag gatcaccggc cacacccccg ccatcagcag
     1621 cgcgtgctgc tcgacccagg tgtcaccgcc gaccggcgtg ccgaacagct cgcgggtcgc
     1681 cgccgccgtg gaggagatcg ggttccacat cgccacccag cccagccagt cgggcatgag
     1741 ctgcggcgcg acgaagatgc tggagatcat ggtcagcggg aaggcgaccg cgaacagacc
     1801 gcccgcggcc tccgggttgg gcacgagcag gccgagccac accccgatcc agatcagcgc
     1861 gaaccgcagc cacagcagca gcccgaacgc ggccaggaag ccgaggcccc cgtccggccg
     1921 ccagcccatg gcgagcgcgg tgagcatcat gatcgccaac tcggcgcagg cgacgagcag
     1981 atcggtgacg ccgcgcccgg ccaccacggc ggaggacgcc atcggcatgg agcggaagcg
     2041 gtcgatgacg cccttggtgg agt
//
LOCUS       DATADN010001193         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_133119, whole genome shotgun sequence.
ACCESSION   DATADN010001193 DATADN010000000
VERSION     DATADN010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133119"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VHH53_15325"
     CDS             complement(<1..1235)
                     /locus_tag="VHH53_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX2291519.1"
                     /translation="MSGRIAIDDVTPVVACGKYPSKAVVGEHIPLSAVVWREGHDAVA
                     ATVVWRGPGDRLVSQVRMQPGEPGTDQFLATVVPDQVGWWSFRVDAWADPWATWRHAI
                     EVKIDAGQDASELENDLHVGARLLDRAARRGGQGHNRPVLVAAAAALRDTGRALGERV
                     GPALATQVTQILDADPLSDLVTRGSSHKMWVDRPRALFSSWYEFFPRSTGGVDDQGNP
                     VHGTFATATRELERIAAMGFDVVYLPPIHPIGEVNRKGANNTTDAGPDDVGSPWAIGS
                     IDGGHDAVHPRLGTLDDFDAFVARVGELGMEVALDFALQCAPDHPWAKEHPEWFVIRP
                     DGTIAYAENPPKRYQDIYPLNFDSDSRGIYTEMLRVMLFWAQRGVRIFRVDNPHTKPP
                     DFWQWLLWQVKNQYPDVLFL"
BASE COUNT          237 a          514 c          498 g          272 t
ORIGIN      
        1 aggaacagca cgtccgggta ttggttcttg acctgccaga gcagccattg ccagaagtcc
       61 ggtggcttgg tgtgcggatt gtccacccgg aatatccgga ctccccgctg tgcccagaac
      121 agcatcaccc gcagcatctc ggtgtaaatg ccgcgggagt cggagtcgaa gttgagaggg
      181 tagatatcct gatagcgctt gggtggattc tcagcgtagg cgatagtgcc gtctggacgg
      241 atgacgaacc attcgggatg ctctttggcc cacggatggt ccggagcgca ctgcagcgcg
      301 aagtccagcg cgacttccat gccgagttca ccaacccgag cgacgaaggc atcgaagtcg
      361 tcaagggtgc ccagccgggg atgcaccgca tcgtgaccgc cgtcgatcga accgatcgcc
      421 cacggcgagc cgacgtcgtc gggtccagcg tccgtggtgt tgttggctcc cttgcggttc
      481 acctctccga tcggatggat cggcggcagg tacaccacgt cgaagcccat tgcggcgatc
      541 cgctccagct cccgggtggc ggtcgcgaac gttccgtgca ccgggttgcc ctggtcgtcc
      601 acgccgccgg tggatcgtgg gaagaactca taccaggagc tgaacagtgc ccgggggcgg
      661 tccacccaca tcttgtgcga gctgcccctg gtgaccaggt cgctgagcgg gtcggcgtcg
      721 aggatctggg tcacctgcgt cgccagtgcg gggccgactc gttcgccgag ggcacgtccg
      781 gtatcccgca atgccgccgc ggcggccacc agtaccggcc ggttgtgtcc ctggccgccc
      841 cggcgcgcgg cgcggtccag caggcgagcg ccgacgtgca agtcgttttc cagctcggat
      901 gcgtcctggc cggcgtctat cttcacctcg atcgcgtgcc gccaggtcgc ccacgggtcg
      961 gcccaggcgt ctacccgaaa gctccaccag cccacctggt ccgggaccac ggtcgcgagg
     1021 aactggtcgg tacctggctc cccgggctgc atccgaacct ggcttaccag ccggtcgccg
     1081 ggaccgcgcc agaccaccgt ggcggccacc gcatcgtggc cctcgcgcca caccacggcc
     1141 gataacggga tgtgttcgcc aaccaccgct ttgctcgggt acttgccgca cgcgaccacg
     1201 ggcgtgacgt cgtcgatggc gatccgacca ctcaccagtt gcctcttccc cgtcggctcc
     1261 cagactggtc cagtctgccg gtagcggccg ttcagtgcgc gttgggtgtg acaatcgcaa
     1321 ccaagtagag gacttcgaca tggccagcag ggccctcggg cttgggtacc cacgcagtac
     1381 ccacaacgcg gtctggtcgc ttctaggtat accccgtatg ccgcgttctt ctggagggtt
     1441 agcctcgcct tgtgcacccg ttcagggagc tgtcggatca tccgcgccgg ctcggggagg
     1501 cgctgcgggc ggtacgcgcc c
//
LOCUS       DATADN010001194         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_133339, whole genome shotgun sequence.
ACCESSION   DATADN010001194 DATADN010000000
VERSION     DATADN010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133339"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..286)
                     /locus_tag="VHH53_15330"
     CDS             complement(125..286)
                     /locus_tag="VHH53_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291520.1"
                     /translation="MEHHPAAAWLAEAATAAERQGGPGMLRYVLLVMIVGCVLTAWFL
                     LRGYRRKDD"
     gene            1114..1275
                     /locus_tag="VHH53_15335"
     CDS             1114..1275
                     /locus_tag="VHH53_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291521.1"
                     /translation="MEYHPPAAWLVEAASAAERQGPGNLLRIVLVVMVVGCVLTAWFL
                     LRGYRRKDD"
BASE COUNT          238 a          529 c          510 g          233 t
ORIGIN      
        1 ggcccatcgt aagcgggagc ccgttccgcg atcaccccga ctccgcctgt tcgtgccgcg
       61 ggcggtcccg cgtgccgccg ccggacccgg tcccgaaggc ccggcgggga accttcgggt
      121 ggcctcagtc gtccttccgt ctgtagccac gcagcaggaa ccacgcggtc agcacacagc
      181 ccacgatcat cacgagcagg acgtaccgca gcatccccgg ccccccttgc cgctcggcgg
      241 cggtcgcggc ctcggcgagc caggcggctg cggggtggtg ctccatggcg gtggtctcct
      301 tcgctgtact gcccgaccac cgtaactccg cctaggctgg gctctgcctc ggggaagcaa
      361 aaggccgcac aaggggccgt aaaggtcaca cagacgcact gggggaagac atgtccgacg
      421 gccacgagca caacgggcac gagcacgtgc cgagcaggca gcgcaggcgc ttccccggaa
      481 tctcctcgcg tacgtacgag cacccggccg accgctcggc cctggtggcg ctgcgcaagc
      541 tgagcggttt cgacacggtg ttcaaggcgc tcagcggcct gctgcccgag cgcagtctgc
      601 ggctgctgtt cctgtccgac tcggtgcgcg tctcggacca gcagttcgcc catctgaacg
      661 tcatgctgcg ggacgcctgc tacatcctgg acctggagaa ggtcccgccg atgtacgtca
      721 cccaggaccc gcagcatgac gttcagatgg gcgaactgct ggtccgagac gcgcaccgag
      781 tcggacagga acagcagcct caggctgcgc tcgggcagca gaccgctgag ggccttgaac
      841 accgtgtcga agccgctcag cttgcgcagc gccaccaggg cggaacggtc ggccggatgc
      901 tcgtacgtac gcgaggagat tccggggaag cgcctgcgct gcctgctcgg cacgttctcg
      961 tgcccgttgt gctcgtggcc gtcggacatg tcttccccca tgtgcgtctg tgtgaccttt
     1021 acggcccttt gtgcggcctt ttgcttcccc gaggcagagc ccagcctagg cggagatacc
     1081 gtggccgggc agtacagcga aggagagcgc gccatggagt accacccccc agccgcctgg
     1141 ctcgtcgagg ccgcgagcgc cgccgagcgg caagggccgg ggaatctgct ccggatcgtc
     1201 ctggtcgtga tggtggtggg ctgtgtgctg accgcgtggt tcctgctgcg tggctacaga
     1261 cggaaggacg actgaggcac tgcgaaggtt ccccgccggg cctcccggga ccgggcccgg
     1321 cggcacccca cgccgtggcc cgcgtcacca acagcggagt cggcgtgatc gcggtcgagg
     1381 cgcccgctta cgatgagccg acgtctttat accgcccaca cgcgataggt cctgccgaag
     1441 atgagcttcc acagcaccgc tgcccagttg gtcaccctcg ccgccgaggg cgaggagcac
     1501 ggcggaaacc
//
LOCUS       DATADN010001195         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_751238, whole genome shotgun sequence.
ACCESSION   DATADN010001195 DATADN010000000
VERSION     DATADN010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751238"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHH53_15340"
     CDS             complement(<1..192)
                     /locus_tag="VHH53_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2291522.1"
                     /translation="MIEFRGVTKRFDDGTVAVDALDLLAGEGQITVLVGPSGCGKTTS
                     LRMVNRIVEPTSGTLLINGC"
     gene            complement(390..>2429)
                     /locus_tag="VHH53_15345"
     CDS             complement(390..>2429)
                     /locus_tag="VHH53_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2291523.1"
                     /translation="EAQRVARIGTWEWEPGSDRLVWSDMMQDLYGAVPAPGQPTYQAY
                     LSLVHPDDREWVDELWRQLLVDHVPVECEHRVLRPDGTVRTFRCHGAVIADTSGSLRL
                     VGTAQDVTDQRAAEIRMMRSSQRFADLVAIAPVGIGIFDDADRLIDANEALCKLLGYD
                     LETLRGMSSEALTHPDDRAERLRTMRSHRAAPCESYTVPQRMLLRADGEAVYCELHIS
                     TSVQDDGQRFMLVVFTDVTERRRVAEALKHRATHDELTGLPNRAAIKEILTELLASPD
                     RDLAVLFCDIDNFKRVNDALGHDAGDELLVALARWLRDGLPEGCTGGRLSGDVFVVIC
                     SDVAAQGGISSLVATVSALLRTAIPLRGQLIRVSAAIGVAVPDAVTDNAATGGAEDLL
                     RFADAAMFEAKRGGTGHFVMANAALMASADRQVELECQLREALVRDELQLYYQPVVDS
                     DGAVLSAEALIRWPHPDHGMVGPDVFLPVAEQGNMMRELDRWVLRTALHEARNWVTPD
                     GRPIAVAVNLSALIPGEPGFVDAVATALAESGLPWDRLVLELVETALVDLSSAARQAM
                     DELVERGVRFAVDDFGTGYSSLARLKELPAQIVKLDRRFVSGIGVDPSDFAVARAVVD
                     MVRAMGRTCVAEGVETATQFQVLRGLGVHAYQGWFFSRAVAGPEFRALLGLGPLR"
BASE COUNT          432 a          882 c          773 g          342 t
ORIGIN      
        1 gcacccgttg atcagcagcg tgcccgaggt gggctcgacg atccggttga ccattcgcag
       61 cgacgtggtc ttgccgcaac ccgagggtcc gaccagcacg gtgatctgcc cctcgccggc
      121 gagcaggtcc agcgcgtcga cggccacggt gccgtcatcg aaccgcttgg tcacgccgcg
      181 gaattcgatc atgtggccgt cttcccggta accggtcgga gcacccgaag gtgtccgggc
      241 accataaccc gggatcggac agccgggcac cagacgcggt cacagcaggc accggacgcg
      301 gtcgcagcgg gcacctgcgc ctgacacgcc ggacaagaag tgcggacacg ccgggcaggg
      361 gcgcggacac gccgggcagg acgggtgagt tagcgaagtg gtccgaggcc gagtagggcg
      421 cggaactcgg gtccggcgac cgcccgagag aaaaaccagc cctggtaggc gtgcacgccg
      481 agtccccgca acacctgaaa ctgggtcgcg gtctccaccc cctccgcgac acaggtgcgg
      541 cccatcgcgc ggaccatgtc gaccaccgca cgggccaccg cgaagtccga cgggtcgact
      601 ccgatcccgc tgacgaaacg ccggtccagc ttgacgatct gtgcgggcag ctccttgagc
      661 cgggccagcg acgagtaacc ggtgccgaaa tcgtcgaccg cgaatcgcac cccgcgttcg
      721 accagctcat ccatcgcttg ccgggccgca ctggacaagt ccaccagcgc cgtctccacc
      781 agctccaaca ccaagcggtc ccatggcagc ccgctctcgg ccagtgcggt agccacggcg
      841 tcgacgaagc cgggctcgcc agggatcagc gcggagaggt tgaccgcgac cgcaatagga
      901 cgcccatcag gcgtcaccca attccgcgcc tcgtgcaacg cggtgcgcag cacccagcgg
      961 tccagctcgc gcatcatgtt gccttgctcg gccaccggca ggaacacgtc cggcccgacc
     1021 atcccgtggt cagggtgtgg ccagcggatc agcgcctccg ctgagagaac cgcaccgtcg
     1081 gaatcgacca ccggctggta gtacagctgc agttcgtcgc gcaccagcgc ctctcgaagc
     1141 tggcattcca gctccacctg ccggtcggcc gacgccatca acgccgcgtt ggccatcacg
     1201 aaatgcccgg tgccgccacg cttggcctcg aacatggccg cgtcggcgaa gcgcagcaag
     1261 tcctccgcgc cgccggtagc ggcattatcg gtaacagcgt ccggcaccgc caccccgatg
     1321 gccgccgaca cccggatcag ctggccacgc agcgggatcg cggtccgcaa caacgccgag
     1381 accgtggcga ccagcgagct gataccaccc tgtgcggcga cgtcggagca gatcaccaca
     1441 aaaacgtcgc cggagagccg gccgccggtg caaccctcgg gcaacccgtc gcgcagccac
     1501 cgcgccaacg cgaccagcag ctcgtcgccg gcgtcatgac ctaatgcatc gttgacccgc
     1561 ttgaagttgt cgatatcgca gaacaacacg gccaggtcgc ggtcgggcga ggcgagcagc
     1621 tcggtcagga tctccttgat cgcggcccgg ttgggcaggc cggtgagctc gtcgtgagtc
     1681 gcccggtgct tcaaggcctc ggccactcgg cggcgctcgg tgacatcagt gaagacgacc
     1741 agcatgaacc gttgcccgtc gtcttgcacc gacgtcgaga tgtgcagctc gcagtacacc
     1801 gcctcaccat cggcgcgcag cagcatccgc tgcggcaccg tatagctctc acagggcgcc
     1861 gcccggtggc tacgcatggt gcgcagccgc tcagctcggt cgtcggggtg ggtgagcgct
     1921 tcggaactca ttccccgaag tgtctccagg tcataaccca gcagcttgca cagcgcctcg
     1981 ttggcgtcga tcagccgatc ggcatcgtcg aagatcccga tgcctaccgg ggcgatcgcc
     2041 accaggtcag cgaagcgctg gctggaacgc atcatccgga tctcggcggc tcgctggtcg
     2101 gtgacgtcct gggcagtccc gaccagccgc aggctcccgg atgtgtcggc gatgaccgcg
     2161 ccgtgacacc ggaaggtacg taccgtgcca tccggccgca gcacccggtg ctcgcactcc
     2221 accggcacgt ggtcgacgag cagctgccgc cacagctcgt ccacccattc ccggtcatcg
     2281 gggtgtacca acgacaggta ggcctgatag gtgggctgac ccggggccgg caccgcccca
     2341 tagaggtctt gcatcatgtc cgaccacacg agccggtccg aacccggctc ccactcccag
     2401 gtaccgattc gggccacccg ctgcgcctc
//
LOCUS       DATADN010001196         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1343317, whole genome shotgun sequence.
ACCESSION   DATADN010001196 DATADN010000000
VERSION     DATADN010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343317"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VHH53_15350"
     CDS             complement(<1..835)
                     /locus_tag="VHH53_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291524.1"
                     /translation="MATVVVLSYRLGAADGVSVAAAQWITALRRLGCQVRTVAGAGAA
                     DRLVPGLAFDTPRPARTRDIEVALRGVDLIVVENVCSLPVNPQVSEVVAHAARGRSAV
                     LRHHDLPWERERYAHLRGWPPDDPGWQHVAISKHSAAELGRRGIDATAVYPGYARIPR
                     RGNREAARAALGVAPHQRLLLQPTRAIPRKNIATVITLAQAIDAVYWLTGPAEEGYGP
                     QLADLLSTAGCPVRRGLPRGLRMADAYAAADAVVLPSTWEGFGLPLIGSALAQRPLAV
                     SN"
     gene            complement(874..>1658)
                     /locus_tag="VHH53_15355"
     CDS             complement(874..>1658)
                     /locus_tag="VHH53_15355"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX2291525.1"
                     /translation="HEPRTGLSMGKHAAVTAKQWEISREAQDELVVASHHRLAAAYQR
                     GFFDDLITPYLGLHRDENLRPGTSIDKLATLKTVFGSDTMTAGNSTPLSDGASVVLLA
                     SEQWAAEQDLPVLAYLVDAQTAAVDFVHGAEGLLTAPVHAVPRLLARHGLGLADLDLI
                     EIHEAFASQVLATLKAWEDADYCKRELGLDAPLGPVDRDKLNVTGSSLAAGHPFAATG
                     ARIVATLAKLLHARGLDTGRCATGLISICAAGGQGVVALLRS"
BASE COUNT          270 a          567 c          572 g          249 t
ORIGIN      
        1 agttgctgac cgcgagcggt cgctgcgcca acgcggatcc aatcagcggc aggccgaaac
       61 cctcccacgt cgagggcagc accaccgcgt ccgcggcggc gtaggcgtcg gccattcgca
      121 gaccccgggg cagtccccgg cgcaccggac aaccggctgt ggagagcaag tcggcgagct
      181 gtggaccgta accctcctcc gcgggcccgg tcagccaata gaccgcgtcg atggcctgcg
      241 ccagcgtgat cacggtggcg atgttcttgc gcgggatcgc tcgcgtgggc tgcaggagta
      301 gccgctgatg tggcgccacc cccagcgcgg ctcgcgccgc ctcccggtta ccgcggcgcg
      361 ggatgcgggc atagccgggg tagacagcgg tcgcgtcgat gccgcgccgg cccagctctg
      421 cggcgctgtg cttgctgatg gcgacgtgct gccagccggg gtcgtccggt ggccagccgc
      481 gcaggtgtgc atagcgctcc cgctcccagg gcagatcgtg gtggcgtagc accgcactac
      541 gcccgcgcgc agcatgcgcg accacctcgc tcacctgcgg gttcactgga agcgagcaga
      601 cgttctccac cacgatgaga tcgacgccgc gaagcgccac ctcgatgtcc ctggtgcgag
      661 ccggacgggg ggtgtcgaaa gcgagccctg gtaccaggcg atccgccgca ccggctccgg
      721 cgacggtgcg cacctggcag ccgagccggc gcagtgccgt gatccactgc gctgcggcaa
      781 ccgacacccc gtcagctgca ccgagccggt aggacagcac aacaactgtc gccactcgtg
      841 agtggcaaac agtgccatgg cactggttgc cactcacgag cgcagcagcg ccacgacccc
      901 ttggccgccg gcagcacaga tcgagatcag cccggtagcg cagcgcccgg tgtcgagtcc
      961 tcgcgcgtgc agcagcttgg cgagggtggc tacgatccgg gcaccggttg cggcgaaggg
     1021 gtgaccggcc gccagcgaag agccggtcac attgagcttg tcccggtcga ctggacccaa
     1081 tggggcgtcg aggcccaact cgcgcttgca gtagtcggca tcttcccagg ccttgagggt
     1141 agccagcact tgcgaagcga acgcctcgtg gatctcgatc aggtccaggt cggccaggcc
     1201 cagcccgtgc cgggcgagca gccgcggcac ggcgtgcacc ggcgccgtga gcaggccctc
     1261 ggcaccgtgc acgaagtcga cagctgcggt ctgcgcgtcc accaggtacg cgagcaccgg
     1321 caggtcctgc tcagcggccc actgttcgct ggccagcagc accaccgatg ctccatcgga
     1381 caatggcgtg gaattacccg cggtcatcgt gtcggagccg aagacggtct tgagcgtcgc
     1441 gagtttgtcg atcgaggtgc ccgggcgcag gttttcgtca cggtgcaacc cgaggtacgg
     1501 ggtaatgaga tcgtcgaaaa aaccacgctg gtaagcagcg gcgagccggt ggtgactggc
     1561 caccaccaac tcgtcttgtg cttcccggga gatctcccac tgcttggcgg tcacggcggc
     1621 gtgtttgccc atcgacaatc ctgtgcgcgg ttcatggt
//
LOCUS       DATADN010001197         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1343343, whole genome shotgun sequence.
ACCESSION   DATADN010001197 DATADN010000000
VERSION     DATADN010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343343"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VHH53_15360"
     CDS             complement(<1..1341)
                     /locus_tag="VHH53_15360"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2291526.1"
                     /translation="MDLASLHFYLFAGVSLLYVVHFGLYLVGANFYDIWQHRRKHVLG
                     RPTRSRAWVECAATATGPDIHPAEVPGLVSIVIAAHNEEQVITRALDSIRMSSYPWYE
                     VIVADDASTDRTEELVQDYRARHPDMDLRLYRMARNVGKGAALTAVLRHHARGEYVMT
                     LDADSIISPDAVAKALSYFEDPCVAGVAANVQIIDEPTVLGVLQRFEHMIGYRSKKLY
                     SVTNCEFVVGGVASTYRMRVLRKVGFYDTDTLTEDIGLSTKVISLGNRRFRLVYGADV
                     VAKTEGVLTFRALARQRYRWKYGSMQNLVKYRHLIGNSSRRYSRTLTLYRMPMAVLSE
                     FTLLVSPLAWTYAIYMSLTQYTPTLVVGAYATITAYTLLTIWMDETLSRRARLRLTMY
                     APTAYFVFYLMDLVQLVAITRCLIRTRSLVQRRDVGNTWSSPKRAGKLVVNARAA"
     gene            complement(1344..>1534)
                     /locus_tag="VHH53_15365"
     CDS             complement(1344..>1534)
                     /locus_tag="VHH53_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291527.1"
                     /translation="AETFGRDAVMIQDLLSFMTTVSLVLWAVVLLTAIVRFVACHVLY
                     RRRDLRTNPSEQLHPGGG"
BASE COUNT          261 a          497 c          528 g          248 t
ORIGIN      
        1 agccgcacgg gcgttcacca ccagcttgcc ggcccgtttc ggcgacgacc aggtgttgcc
       61 cacgtccctg cgctggacga gggagcgtgt cctgatcagg cagcgggtga tcgccaccag
      121 ctggaccagg tccatgaggt agaagacgaa gtaggcggtg ggcgcgtaca tcgtcaggcg
      181 caggcgcgcc cgccggctga gggtctcgtc catccagatg gtgagcaggg tgtaggcggt
      241 gatcgtcgcg tacgcaccca ccaccagagt gggggtgtac tgcgtcagcg acatgtagat
      301 cgcgtacgtc cacgccagcg gggagacgag gagcgtgaac tcgctcagca ccgccatcgg
      361 catccggtac agggtcagcg tgcggctgta ccggcggctc gagttcccga tcagatggcg
      421 atacttgacc aggttctgca tgctgccgta cttccaccgg taacgctggc gggcgagtgc
      481 gcggaaggtc aggacgcctt ccgtcttggc caccacgtca gcgccgtaga ccagccggaa
      541 ccgccggttc ccgaggctga tcaccttcgt gctcaggccg atgtcctcgg tcagcgtgtc
      601 ggtgtcgtag aagccgacct tgcgcaggac ccgcatgcgg tacgtggagg cgaccccgcc
      661 caccacgaac tcgcagttgg tcaccgagta cagcttcttg gagcggtagc cgatcatgtg
      721 ctcgaaccgc tgcagcactc cgaggacggt cggctcgtcg atgatctgga cgttggcggc
      781 gacgccggcc acgcaggggt cctcgaaata ggagagggcc ttcgccaccg cgtccggcga
      841 gatgatggaa tcggcgtcca gcgtcatgac gtactcgccg cgggcgtggt ggcgcaggac
      901 cgcggtcagc gcggcgccct tcccgacgtt cctcgccatc cggtagaggc gcaggtccat
      961 gtccgggtgc cgggcccggt agtcctgcac caactcctcc gtccggtcgg tcgaggcgtc
     1021 gtcggcgacg atcacctcgt accacgggta gctgctcatc cggatggagt cgagggcccg
     1081 ggtgatcacc tgctcctcgt tgtgcgccgc gatcacgatg ctaaccaggc cgggcacctc
     1141 agcgggatgg atgtccggtc cggtggccgt ggcggcacac tcgacccacg cccgagatcg
     1201 ggtgggccga ccgagcacgt gctttcgccg gtgctgccag atgtcgtaga agttggcgcc
     1261 gacgagatag agcccgaagt gcaccacgta cagcaggctg acgcccgcga acaggtagaa
     1321 gtggaggctc gcgaggtcca tgctcacccg ccccccggat ggagctgctc gctcgggttc
     1381 gtccggaggt cgcggcgccg gtacagcacg tggcaggcga cgaaacgcac gatggccgtc
     1441 agcaggacca ctgcccagag caccaggctc acggtcgtca tgaacgacaa caggtcctgg
     1501 atcatgactg cgtcacgccc gaaggtctcg gccg
//
LOCUS       DATADN010001198         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_290558, whole genome shotgun sequence.
ACCESSION   DATADN010001198 DATADN010000000
VERSION     DATADN010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290558"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VHH53_15370"
     CDS             <1..1118
                     /locus_tag="VHH53_15370"
                     /inference="COORDINATES: protein motif:HMM:NF026246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinosyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2291528.1"
                     /translation="LDSVLLWGMAVVFVALVLGWFTGTRDPRLATLQAVPIVVVASLA
                     ATTGYAVGTFGLAAVQGMPRESMWARAFADPTGADCGAAGAVRVLDPFTAQLLPAAGP
                     PPPASDGFVEGGGYYPGNRPQGSATDQVWGSLIVRDGQTAERNTGEMVTGWYALPANL
                     AADTVGTAVTVLAAGTLTDGNSLDAVYGVRSGDSVITVGSQPLIGIARDPSWRTFTLT
                     APDGADVVRLQAVDVTGALHGWLAFTAPAVAHPVVLQEFLPDQAPVALGWQLAFAYPC
                     QRQPHVVNGITEAPSYAVLWGEGALAGLIDPAWQAPQGGVFGHVARTQSVLQLATVGP
                     VTPFIQVYAFGTSLGRDGYILSTARRTVAGASTATTS"
     gene            complement(1237..>1893)
                     /locus_tag="VHH53_15375"
     CDS             complement(1237..>1893)
                     /locus_tag="VHH53_15375"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX2291529.1"
                     /translation="ELLGARIAPRDADAEVVAGVDLRECRGLRVHRERLADDEVETVD
                     GLLVTSAIRTAYDLARWSTLVEGVVAADALGRIGRFGPNALVRLAARYPGVRWRKRVP
                     LVAELMDPRAESAMETRCRLSLVLRGLPRPELQYRVCDELGDHVAWLDIAYPSARAGV
                     EFDGGHHQDTATFRADLARHNRLAALGWTVLRASAADVLRNPDRFAAQVHAVLRQRAA
                     "
BASE COUNT          274 a          686 c          648 g          285 t
ORIGIN      
        1 ccctcgacag cgtgctgctc tggggaatgg cagtggtgtt cgtggcgctc gtcttgggct
       61 ggtttaccgg cacccgggac ccgcggctgg ccacgctgca ggcagtaccg atcgtcgtgg
      121 ttgcatcgtt ggcggccacc accgggtacg ccgtcggaac gttcggtctc gccgccgtgc
      181 agggcatgcc ccgcgagtcc atgtgggcac gggcgttcgc cgatccgacc ggggccgact
      241 gcggtgctgc cggggcggtg cgggtactgg acccgttcac tgcgcagctg ctgccggctg
      301 ccggcccgcc gccgcctgca tccgacggct tcgttgaggg cggcggctac taccccggca
      361 accggccgca gggcagcgcg accgaccagg tctggggaag cctcatcgtc cgcgacggcc
      421 agaccgccga gcggaacacc ggggagatgg tcaccggctg gtacgcgctg ccggcgaacc
      481 tcgccgccga cacggttggc acggccgtca cggtgctcgc ggccgggacg ctcaccgacg
      541 gcaactcgct cgacgccgtc tacggggtcc gctccggcga ctcggtgatc acagtgggca
      601 gccagccgct gatcggcatc gcccgcgacc cgtcgtggcg caccttcacg ttgacggctc
      661 cggacggcgc ggatgtcgtt cggctgcaag ccgttgacgt gaccggagcg ctgcacggct
      721 ggttggcgtt caccgcgccc gccgtggcgc acccggtcgt gttgcaggag ttcctgccgg
      781 accaggcgcc ggtcgcactg ggctggcagc tggcgttcgc ttacccctgc cagcgacagc
      841 cgcacgtcgt caacggcatc accgaggccc cctcatacgc ggtgttgtgg ggtgagggcg
      901 ctctggccgg gttgatcgac ccagcgtggc aggcgccgca gggaggcgtg ttcggccacg
      961 tggcgcgcac gcagtcggtg ctgcagctgg ccaccgtcgg cccggtcacc ccgttcatcc
     1021 aggtgtatgc gttcggcacc agcctcggcc gtgacggcta catcctcagc accgctcggc
     1081 gcaccgttgc cggggcatcc accgccacca ccagctagcc aaccgcgcac tgccgtcgcc
     1141 gagttcgaca tcagagcagt cgagtcggca gcggacagcc ctgatgtcga actcggcgca
     1201 tgcagagcgg tcagccgcac catcagatcg tgcgggtcag gcggcgcgtt ggcggagcac
     1261 cgcgtggact tgagcggcga atcgatccgg gttgcgtagc acgtcggcgg cgctggcccg
     1321 cagcacagtc cagcccagcg cggcgagccg gttgtgcctg gccaggtctg cccggaatgt
     1381 cgccgtgtcc tggtggtgcc ctccgtcgaa ctccacaccg gcccgcgcac tcgggtaggc
     1441 gatgtccagc caggccacgt gatctccgag ctcgtcgcat actcggtatt gcagctcggg
     1501 acgcgggagc ccgcgcagaa cgagggataa cctgcaccga gtctccatgg cagattccgc
     1561 gcgcggatcc atcagttcgg cgaccagcgg aacccgcttg cgccaccgca cccccgggta
     1621 acgagcggcc agcctgacca gcgcgttcgg cccaaagcgc ccgatccggc caagggcgtc
     1681 ggccgccacc acaccctcca cgagggtcga ccaacgcgcc aggtcatagg cggttcgaat
     1741 cgcggacgtg accagtagcc catccacggt ctcgacctca tcgtcggcaa gtcgctcccg
     1801 atgtacccgc agtccgcggc actcgcgcag atcgacgccg gccaccacct cggcgtccgc
     1861 atcacgaggc gctatccgag caccgagcag ttc
//
LOCUS       DATADN010001199         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1343582, whole genome shotgun sequence.
ACCESSION   DATADN010001199 DATADN010000000
VERSION     DATADN010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343582"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VHH53_15380"
     CDS             <1..972
                     /locus_tag="VHH53_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX2291530.1"
                     /translation="QLRPVYLPPDPCGRRSYVAGEIAQHDFWFPDIELPVGYGQTRTA
                     TQLPVLTMVTGYSRWLSAVLIPTRTAEDLFAGWWGHLQGLGAVPRVLVWDGEGAIGRR
                     RGGRTELTEVCQGFRGTLGAKVLVLRPADPEAKGLLERAHDYLERSFLPGWVFASPAD
                     FNAQLAAWLAVVNQRRRRALGCAPCDRIAADKAAMLALPPVAPVLGWRTTTRLARDHY
                     VRLDSNDYSVHPGVIGRRIEVVADLSRVRAHCDGQLVADHERLWAKHQTLSAAEHVAA
                     ARALRRERIGLLRPAPQPAVEIRALADYDTALGIDDATDVDDEGGVA"
     gene            1029..>1713
                     /gene="istB"
                     /locus_tag="VHH53_15385"
     CDS             1029..>1713
                     /gene="istB"
                     /locus_tag="VHH53_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX2291531.1"
                     /translation="MSSTAARDLRGEVAFLTRALKAPTLRESVPRLAERARAESWSHE
                     EFLVACLQREVAARESHGGEGRIRAARFPARKSLEEFDFDHARGLKRDTIAHLGTLDF
                     VTARDNAVFLGPPGTGKTHLATGLAIRACQAGHRVLFATAAQWVDRLATAHHAGTLQT
                     ELARLARYPLLVIDEVGYIPFEPEAANLFFQLVSSRYERASLIVTSNRVFGRWGEVFG
                     DDVVAAAMID"
BASE COUNT          241 a          556 c          625 g          291 t
ORIGIN      
        1 cagttgcgtc cggtgtatct gccgccggat ccgtgcggtc gtaggagtta tgtggctggg
       61 gagatcgctc agcacgattt ctggtttccc gatatcgagc tgccggtggg ctatggccag
      121 acccggaccg ccacgcagct gccggtgttg acgatggtga cgggttattc gcggtggttg
      181 tcggcggtgt tgatcccgac tcggaccgcg gaggacttgt tcgctggctg gtgggggcat
      241 ctgcaggggt tgggcgcggt gcctcgagtg ttggtgtggg atggggaggg cgccatcggg
      301 cgccggcgtg gtgggcggac ggagttgacc gaggtgtgtc agggtttccg gggcacgctc
      361 ggggcgaagg tgctggtcct gcgcccggcc gaccccgagg ccaaggggct gcttgagcgg
      421 gcccacgatt atctggagcg ctcgtttttg ccggggtggg tgttcgcttc gccggcggac
      481 ttcaacgctc agctcgccgc ctggttggcg gtggtgaatc agcgtcggcg ccgggcgttg
      541 ggttgtgcgc cgtgtgaccg gatcgctgcg gataaggcgg cgatgctggc gttgccgccg
      601 gtggcgccgg tgttggggtg gcggacaacg acccggctgg cccgtgatca ctacgtgcgt
      661 ctagacagca acgactactc ggtacacccc ggcgtgatcg ggcggcggat cgaggtcgtg
      721 gccgacctga gccgggtgcg ggcacactgc gatgggcagc tggtcgccga ccacgagagg
      781 ttgtgggcca aacaccagac cctctcggct gccgaacatg tcgccgccgc gcgagcgctg
      841 cgccgcgaac gcatcggcct gctgcgcccc gccccgcagc cggccgtcga gatccgcgcc
      901 ctggccgact acgacaccgc cctgggcatc gacgacgcca ccgacgtcga cgacgagggt
      961 ggggtggcgt gatggtcacc gccgccaaga ccacgaccag caccggttgc agcgcccggg
     1021 ccgccacgat gagcagcacc gcggctcggg acctgcgcgg ggaggtagcg tttttgactc
     1081 gcgcgcttaa ggcccccact ctgcgggaat cggtgccacg gctggccgag cgggcccgcg
     1141 ccgagtcgtg gagccatgag gagttcctgg tggcatgtct gcagcgcgag gtcgccgccc
     1201 gggagtctca cggcggggaa ggccgcatcc gcgccgcgcg gttcccagcc cgtaaaagcc
     1261 tcgaggagtt cgacttcgac cacgcccgcg ggctcaaacg cgacaccatc gcccatctgg
     1321 gcaccctgga cttcgtcacc gcccgcgaca acgcggtgtt tctgggtccg cccgggaccg
     1381 ggaaaaccca cctcgccacc gggctggcca tccgagcctg ccaggccggg caccgggtcc
     1441 tgttcgctac cgccgcgcag tgggtcgacc gcctggccac cgctcaccac gccggaacac
     1501 tgcagaccga gctggcccgg ctggcccgct acccgctgct ggtcatcgac gaagtgggct
     1561 atatcccctt cgaacccgaa gcggcgaacc tgttcttcca gcttgtctcg tcccgatatg
     1621 aaagggccag cttgatcgtg acctccaaca gggtcttcgg ccgctgggga gaggtcttcg
     1681 gagatgacgt tgtcgccgcc gcgatgatcg aca
//
LOCUS       DATADN010001200         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_751604, whole genome shotgun sequence.
ACCESSION   DATADN010001200 DATADN010000000
VERSION     DATADN010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751604"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VHH53_15390"
     CDS             complement(<1..1350)
                     /locus_tag="VHH53_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding monooxygenase"
                     /protein_id="HEX2291532.1"
                     /translation="MKTATVGDHAVVLGASMAGLLTARVLTDAYHRVTVVERDILPHD
                     LAGRRGVPQGRHLHALHARGRELLDELFPGFTEQLVQAGAVTADTLGDARIHVSGQLL
                     RQADIGLPALSASRPFIEGHVRQRVRALPAVRFVEGCDVLSLVTGDEQHRVTGVRLGH
                     RDDRGSQHTVPADVVVDATGRGSRTPVWLEQFGYPRPAIDRVEIGLGYASRRYRLRPG
                     ALGNDAAILTGPTPINPRAGAISPVEGGQHMVTLAGILGDHPPLDPAGFGAFAASICY
                     PDLAEALAHAIPVDDPVGFRFPVSVRHRYERLRRFPVGLLVIGDAVCSFNPIYGQGMT
                     VAAMQAVTLRDLLRGGPPPAAHKYFRRIAKMIDTPWDINVGADLAFPGVPGRRSTKVR
                     MVNAYLPRLHAAASTDSSLGRAFMRVVGMLDRPEGLVRPDQALRVLWAHLRGTPAPAD
                     "
BASE COUNT          258 a          566 c          547 g          255 t
ORIGIN      
        1 atccgctggt gctggtgtgc ctcgcaggtg tgcccacagc acgcgcaatg cctgatccgg
       61 ccgcaccagc ccttcgggcc ggtcgagcat tcccaccacg cgcatgaatg cccgccccag
      121 cgagctatcg gtgctggcgg cggcatgcag tcgcggcagg taggcgttga ccatccgcac
      181 cttggtgcta cgccggcccg gcacgccggg gaatgccaga tcggcgccga cgttgatgtc
      241 ccacggcgtg tcgatcatct tcgcgatccg ccggaagtac ttgtgcgccg cgggcggtgg
      301 cccaccgcgc agcaagtccc gcagggtcac tgcctgcatg gcggccaccg tcatgccttg
      361 cccgtagatg ggattgaacg agcacaccgc gtccccgatc accagcaggc caaccgggaa
      421 acggcgcaac cgctcatagc ggtgacgcac gctgaccggg aagcggaaac ccactgggtc
      481 atcgaccggg atggcatgcg ccagcgcctc ggcaaggtcg gggtagcaga tgctggccgc
      541 gaacgcaccg aacccagccg ggtccagcgg cggatggtcg ccaaggatgc cggccagcgt
      601 gaccatgtgt tggccgccct ccacggggct gatagctccc gcccgcgggt tgatcggtgt
      661 cggcccggtg aggatggccg cgtcattacc caaggcgccg gggcgcagcc ggtagcgacg
      721 gctggcatag cccagcccga tttcgactcg gtcgatcgcg ggccgcgggt agccgaactg
      781 ctccagccac accggcgtgc gcgaccccct gccggtggcg tcaacgacca catcagcggg
      841 caccgtgtgc tgcgaaccgc ggtcgtcgcg gtgccccaac cgcaccccgg tgacccggtg
      901 ctgctcgtcg ccagtgacca ggctgagcac gtcacagccc tcgacgaaac gcaccgccgg
      961 cagcgcccgc acccgttggc ggacgtggcc ctcgatgaat gggcggctgg cgctcagggc
     1021 gggcaggccg atgtcggctt ggcgcagcag ttgaccagac acgtggattc gggcgtcgcc
     1081 taaagtgtcc gcggttaccg cgccggcctg caccagttgc tcggtgaagc cggggaacag
     1141 ctcgtcgagc agttcacggc cgcgggcgtg cagtgcatgc aggtgacggc cctgtggcac
     1201 cccccggcgg ccggcgagat cgtgcggcag gatgtcccgc tcgaccactg tcacccgatg
     1261 gtaggcatcg gtcagaaccc gggcggtcag caggccggcc atgcttgctc ccagcaccac
     1321 ggcgtggtca ccgacggtgg ccgtcttcat gaagttcgtg gtcccgtccc gagtgctccg
     1381 tggctcgcag tgtcgacacg ccgcgacggc atggcgttgg cgggacacgc cgggccgagg
     1441 tcgcaagaaa ttgttccata cctgcgcaca gggtttgagc agtgcgaacg agaaaaatcg
     1501 atcagttttc cccatgttgt ccccactgtc tgtcgcacta tgagcggggt caggcgctcc
     1561 gtcctctacc gcgtccccat gttgtcccca gcggtcgctt gctcatgcct tcaggccgcc
     1621 ctaacc
//
LOCUS       DATADN010001201         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_891930, whole genome shotgun sequence.
ACCESSION   DATADN010001201 DATADN010000000
VERSION     DATADN010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891930"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..151)
                     /locus_tag="VHH53_15395"
                     /pseudo
     CDS             complement(1..151)
                     /locus_tag="VHH53_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(230..1093)
                     /locus_tag="VHH53_15400"
     CDS             complement(230..1093)
                     /locus_tag="VHH53_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2291533.1"
                     /translation="MIPDVHAAQGSAPGYRAAGTLPLRVELVRQLRRRRTVLTLGFLA
                     LLPFLLWLAFEVGQDESERGGTTLVDLATASGSNFVVFTMFSTVGFLLVVVVALFFGD
                     TIASEASWSTLRYLLAAPVPRGRLLRQKAMVAALLSAFALVLLPAVAMAVGVVAYGAG
                     DLVSPTGQSLEFLPTASRLALAPAYLVLHLAWVAGLALLLSVTTDAPLGAVGGAVMVS
                     ILSQILETIDALGHLRDYLPTRFSSAWADLLASPIGWSDLASGAFSGVAYATVFGLAA
                     WWRFATKDITS"
     gene            complement(1090..1576)
                     /locus_tag="VHH53_15405"
                     /pseudo
     CDS             complement(1090..1576)
                     /locus_tag="VHH53_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227899.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          274 a          556 c          542 g          204 t
ORIGIN      
        1 cccgctctcc cggatgaggt acgcccgcag caccgccgcg gagcagccac gcgtacgggg
       61 tggtgccggt gtgtgcccgg aagtgccggg cgaaggtacg cggtgacatg tgcgctcggg
      121 tggccagtgc ttcgaccgtg agcggtttcg cggcgttgct ctgctacgac ggcgaacgtg
      181 tcttcgtcat caccgggcgc ggtgccccgg ctgatctggt cgtgtcgcgt caactggtga
      241 tgtccttggt ggcgaaccgc caccaagcgg caagcccgaa gaccgtcgcg taggccaccc
      301 cagagaacgc tccgctggcc aggtcactcc agccgatggg ggaggccaag aggtcagccc
      361 acgcggagct gaaccgggtg ggcaggtagt cccggaggtg accgagcgcg tcgatggtct
      421 ccaggatctg ggacaggatc gacaccatca ccgcaccacc gaccgccccg agcggcgcat
      481 cggtggtcac cgacagcagc agtgccagcc cagctaccca cgccaggtgc agcaccaggt
      541 aagcgggggc cagcgccagc cggctcgcgg tgggaaggaa ctccaatgac tggccggtgg
      601 gggagaccag gtcgccggct ccgtaggcga ccacaccgac cgccatcgcg accgcgggca
      661 gcagcaccag ggcgaaggcc gacagcagcg cggccaccat cgccttctgc cgcagcagcc
      721 gcccgcgggg taccggggca gccagcaggt agcgcagggt cgaccaggac gcctcgctgg
      781 caatcgtgtc accgaagaac agcgccacca cgacaaccag caggaagccc accgtggaga
      841 acatcgtgaa caccacgaag ttggacccgc tggcggtggc gaggtcgacc agggtggtac
      901 cgccgcgctc gctctcgtct tgcccgacct cgaaggccag ccagagcagg aagggcagca
      961 gtgcgagaaa ccccagggtg agtactgtcc gcctgcgccg cagctggcgg accagctcca
     1021 ctcgcaacgg caaagtgcca gcggcccggt aaccgggtgc cgacccctgg gcggcatgca
     1081 cgtcggggat cactcgacag tctcctcacc tacgagctgc atgaaggcct cctccagcgg
     1141 gtggcgggcg gccactgcct cgaccccgat cccggccccc accagcgcgg ccaccgcgac
     1201 cgcgcggggc agcccgtcga gttgggcgtg cacgacgcaa cgttcgactt ccatcacgtc
     1261 aactcccgcg aggtcacgta gcactgcggc ggccagcgcc gcgtcgtcca cggtaaagct
     1321 caccgtgctg gtgcccgcca cgacctcggt gacggtgccg gcggccacca accgaccccg
     1381 gtgcatcacc acgacatggc tgcaggtctg ctccacctcg gcgagcaggt ggctggagat
     1441 gagcaccgtc cggccgctgg cggcgtaatc ccgcaggacc tcgcgcatgg cgtggatctg
     1501 tggtggggtc caggccgttg gtgggctcat cgagcaccag cagctccggc ttcccgagca
     1561 tggcctgcgc gatcgc
//
LOCUS       DATADN010001202         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_290751, whole genome shotgun sequence.
ACCESSION   DATADN010001202 DATADN010000000
VERSION     DATADN010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290751"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VHH53_15410"
     CDS             complement(<1..933)
                     /locus_tag="VHH53_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2291534.1"
                     /translation="MELSEEHAALRATVEEFAHKEVAPVIGELYQRGEFPYELIAKMG
                     AMGLFGLPFPEEYGGMGGDYFALCLALEELARVDSSVAITLEDSVSLGAMPIFRFGTA
                     EQKQRWLPELCAGHKLGAFGLTEPGGGSDARATRTTARCDDKEWVINGSKAFITNSGT
                     DITSVVTVTAITGVSGGRKEISAIIVPARHPGFTVSESYYKVGWNCSDTRGLFFDDCR
                     VPVDNLLGERGRGYAQFLSILDEGRIAIAALGVGLAQGCIDESVSYARQRRAFGHAIG
                     SYQAIQFKIADMEVRAHTARLAYYQAAARMLRGQP"
     gene            complement(965..>2050)
                     /locus_tag="VHH53_15415"
     CDS             complement(965..>2050)
                     /locus_tag="VHH53_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2291535.1"
                     /translation="EHPVTELVTGLDLVELQLRVAAGEPLALCQGDVRIRGHAVQARI
                     YAEDPSGGFLPTGGRVLSVREPTGPGIRVDSGLVPGATVSSDYDPLLAKVIAYGPDRD
                     IALRRLDTALTATSILGLGVNTGFLRALLADSDVRAGRLDTGLVERRVDGWVGEALPD
                     DVAAAAGLLGLLALEPCGPVLDPFALPGGWRIGEPAWTRWRLLATEPVEVRTRGRAGA
                     ADVAVGQGKPVGASAWWDGADLVVTLDGITRRYTCARDGAEVWLGRDGHSWMLRELES
                     ARRVASDVGGAGPVLAPMPGTVTAVEVDEGQQVTAGARLLVIEAMKMEHLLTAPIDGV
                     IRELHARPGGTVERDAVLAVVEPVYTR"
BASE COUNT          339 a          745 c          667 g          299 t
ORIGIN      
        1 cggttggcca cgcagcatcc gcgccgcagc ctggtagtag gccagccggg cggtgtgcgc
       61 ccgcacctcc atgtcggcga tcttgaactg gattgcctgg tagctgccga tggcgtggcc
      121 aaaagcgcgc cgttgccggg cgtaggacac ggactcgtcg atgcagccct gtgccaggcc
      181 gacgcccagc gccgcgatag cgatacggcc ctcgtccagg atggacagaa attgcgcgta
      241 gccccggccg cgctcaccga gcaggttgtc taccggcacc cggcagtcgt cgaagaacag
      301 tccacgggta tccgagcagt tccagccgac cttgtagtaa ctctcggaga ccgtgaagcc
      361 cggatgccga gccgggacga tgatggcgga gatctccttg cggccgccgc tgacgccggt
      421 gatcgccgtg acggtgacca ctgacgtgat gtctgtaccg gagttggtga tgaatgcctt
      481 ggagccgttg atcacccatt ccttgtcgtc gcaccgggca gtggtgcggg tagcgcgcgc
      541 gtcggaaccg ccgccgggtt cggtgagacc gaaggcaccc agtttgtggc cggcgcacag
      601 ctccggcagc caccgctgct tctgctccgc ggtgccgaac cggaagattg gcattgcacc
      661 cagcgacacg ctgtcttcca gcgtgatggc caccgacgag tccacccgcg caagttcctc
      721 caatgccagg catagcgcga agtagtcgcc gcccatcccg ccgtactctt cggggaaggg
      781 caacccgaac aaccccatcg cacccatctt cgcgatcagc tcgtagggga actcaccgcg
      841 ctggtacagc tccccgatga ccggagcgac ctccttgtgc gcgaactcct cgacagtggc
      901 gcgcaacgcc gcatgctcct cactcagctc cacggcgtgt cctcctctcc cgcccggtgt
      961 gtcgtcatcg cgtgtacacg ggttcgacga cggcgagcac tgcgtcgcgc tcgacggtgc
     1021 cgccgggacg ggcatgcagc tcgcggatca ccccgtcaat cggtgcggtg agcaggtgct
     1081 ccatcttcat cgcctcgatg acgagcaacc gggcgcccgc agtgacctgc tggccctcgt
     1141 cgacctcgac ggcggtgacg gtgccgggca tcggagcgag caccgggccg gcaccgccaa
     1201 cgtcactggc aacgcgccgc gcgctttcga gttcgcgcag catccagctg tgcccgtcgc
     1261 ggcctaacca cacctcggcg ccgtcccgag cgcaggtgta gcggcgggtg atcccgtcca
     1321 aggtgaccac caggtctgca ccatcccacc acgccgacgc gccgaccggt ttgccctgcc
     1381 cgacggcgac atcggcggca ccggcccgcc cccgagtgcg gacctccacc ggctcggtag
     1441 ccagcagccg ccagcgggtc caggccggtt cgccgatccg ccagccaccg ggcagtgcga
     1501 acggatccag gaccgggccg cagggctcca gcgccaacaa cccgagcaac ccggccgcag
     1561 cggccacgtc atcgggcagg gcctcgccga cccagccatc gacgcgacgc tcgaccaacc
     1621 cggtgtccag ccgaccggcg cgtacgtcgg aatcggccag cagcgcgcgc agaaatcccg
     1681 tgttaacccc caggccgagg atgctggttg ccgttagcgc ggtgtccagg cggcgcagcg
     1741 cgatgtcccg atccggacca tacgcgatga ccttggccag cagcggatcg taatcactgg
     1801 agacggtggc gcccggcacc agcccggagt cgacccggat gccgggtccg gtgggctcac
     1861 gcacagacaa cacccggccc ccagtgggca ggaagccacc ggaagggtcc tcggcgtaga
     1921 tccgtgcctg cacggcgtgg ccgcgaatgc ggacgtcgcc ctggcacagt gctagtggct
     1981 ccccggcggc cacccgcagc tgcagctcga ccaggtccag gccggtgacc agctcggtga
     2041 ccggatgctc
//
LOCUS       DATADN010001203         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_290873, whole genome shotgun sequence.
ACCESSION   DATADN010001203 DATADN010000000
VERSION     DATADN010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290873"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VHH53_15420"
     CDS             complement(<1..831)
                     /locus_tag="VHH53_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2291536.1"
                     /translation="MDRATVETKVFGVLRWVVIAILILVTLFPFYYMLLLSLRPIDEV
                     LQNPGRIWAGPAEWTVQTYREVLTPASGGGQGFLRFLANSAMVSAAATVLTLLVSIPG
                     AYAVSRLKFVGRRQVHFLFLAVYLFPSILLAIPLFVLFTRLGLRGSLAGLTIVYIAQT
                     VPVSIYMLRNYLATIPESIEEAAAMDGYSRLQTLWKVILPLAAPAVMANALYVFMIAW
                     NEFLFALLFLVEDRSNWTVSLGLAQLSGGIEVSKTVLMAGSVVLTVPIMIVFFAAERM
                     L"
     gene            complement(831..>1619)
                     /locus_tag="VHH53_15425"
     CDS             complement(831..>1619)
                     /locus_tag="VHH53_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2291537.1"
                     /translation="GLFGRYTLANIEAVVSSPGFWTSLWTTLAYTVGATILSIGLGLI
                     AALALRAPFRGRTLVRASVLLPYVAPVVAVTFVWEILLSPQFGLANNWGTRFLGWDRP
                     IAFLSQREYTMSLFGWQVDVPLALFTVIAFEAWRYFPFAFLFLLARLQAVPAELEEAA
                     LVDGAAPTQNFRHVLLPQLGPVIALLCVLRFIFTFNKFDDVYLLTGGGAGTEVVSVRV
                     YEFLTARFDIGAASAQALVLAAGLVVLLGIYLKFLAPRFEGVQD"
BASE COUNT          327 a          558 c          546 g          188 t
ORIGIN      
        1 cagcatccgc tccgcggcga agaagacgat catgatgggt acggtgagga ccaccgagcc
       61 cgccatgagc acggtcttcg acacctcgat gccgccggaa agctgggcca agccgagcga
      121 aacggtccag ttcgaccggt cctcgaccag gaacagcagc gcgaacagga actcgttcca
      181 ggcgatcatg aagacgtaca gcgcgttcgc catgacggca ggggcggcca gcggcaggat
      241 caccttccac agcgtctgca gccgcgagta gccgtccatc gcggcggcct cctcgatgct
      301 ctccgggatg gtcgccaggt agttgcgcag catgtagatc gacaccggca cggtctgcgc
      361 gatgtagacg atggtcagac cggccagcga accgcgcagc ccgagccggg tgaacagcac
      421 gaacagcggg atcgccagca ggatcgacgg gaacaggtac accgccagga acaggaagtg
      481 cacctgccgc cggccgacga acttcagccg gctgaccgcg tacgcccccg ggatcgacac
      541 cagcagcgtg aggaccgtcg cggcggccga gaccatcgcg gagttggcga ggaagcgcag
      601 gaagccctgg ccgccgccgg acgcgggggt gagcacctcg cggtacgtct gcacggtcca
      661 ctccgccggg ccggcccaga tccggcccgg gttctgcagc acctcgtcga tcggccgcag
      721 cgacagcagc agcatgtagt agaacgggaa cagcgtcacc aggatcagga tcgcgatgac
      781 gacccagcgc aggacgccga acaccttggt ctcgacggtg gcccggtcca tcagtcctgc
      841 accccctcga agcgtggcgc caggaacttc agatagatcc ccagcagcac gaccaagccg
      901 gccgccagca ccagggcctg cgcggacgcc gccccgatgt cgaagcgggc ggtgaggaac
      961 tcgtacaccc ggacgctgac cacctcggtg cccgctccgc cgccggtgag caggtacacg
     1021 tcgtcgaact tgttgaacgt gaagatgaac cgcagcacgc acagcagggc gatcaccggg
     1081 ccgagctgcg gcagcaggac gtgccggaag ttctgggtcg gcgcggcgcc gtccaccagc
     1141 gccgcctcct ccagttcggc gggcaccgcc tgcagccggg ccagcaggaa gaggaacgcg
     1201 aacgggaagt accgccacgc ctcgaacgcg atcaccgtga acagcgccag cggcacgtcg
     1261 acctgccagc cgaacagcga cattgtgtat tcgcgctggc tgaggaaggc gatcggccgg
     1321 tcccagccga ggaacctcgt gccccagttg ttcgcgagcc cgaactgcgg gctgagcagg
     1381 atctcccaca cgaacgtcac ggccaccacg ggcgcgacgt acggcagcag gacggacgcg
     1441 cggaccagcg tccgtccccg gaacggtgcg cgcagcgcca gcgcggcgat caggccgagc
     1501 ccgatcgaca ggatcgtggc gcccacggtg taggccaacg tggtccacag cgacgtccag
     1561 aagccgggcg aggacaccac tgcctcgatg ttggccaggg tgtaccggcc gaacaggcc
//
LOCUS       DATADN010001204         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1344379, whole genome shotgun sequence.
ACCESSION   DATADN010001204 DATADN010000000
VERSION     DATADN010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344379"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VHH53_15430"
     CDS             complement(<1..1163)
                     /locus_tag="VHH53_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX2291538.1"
                     /translation="MLDRIGSGGMGVVWQARDERLQRLVAVKQLLLAPDLSPGETEEA
                     RLRALREARIAARLQHPNAIVVYDVAEHDGEPCLVMEYLPARSLAAVLNERCFLPVDE
                     VASIGAQVASALAAAHAAQIVHRDVKPGNILIADDGIAKITDFGVSRAAGDVTVTQTG
                     MMAGTPAYLAPEVARGQVPTTASDVFSLGATLYAAVEGRAPFGEGTNQLALLHAVAAG
                     EVPPPLHAGPLFAVLMNLLAKDPAARPDMHRACLELELAAARSTGPVRRPATAAPPAT
                     RVAASIDLPAPSTSLPTVPSRPRPVSPRPAGRPSAGRPSGGPAPAAPLRQTRPQAGVL
                     VGIVLAVLALAGVGTLVTAGSSGERGTPSAKQEATAAPAAPPVSPLGVLPAPP"
     gene            1356..>1538
                     /locus_tag="VHH53_15435"
     CDS             1356..>1538
                     /locus_tag="VHH53_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291539.1"
                     /translation="MTLTEALPGGSGPDEVLDTFVNWVSGQGLTLYPAQEDALIEIVS
                     GSNVILATPTGSGKSLV"
BASE COUNT          233 a          553 c          531 g          221 t
ORIGIN      
        1 tgtggtggcg cggggagaac accgagtgga ctcaccggcg gtgccgccgg cgctgcggtg
       61 gcctcctgct tggcggaagg agtgcctcgc tccccggatg acccggccgt caccagcgtc
      121 ccgacgccgg ccaacgcgag caccgccagg acgatcccga caagcacgcc cgcttgcggc
      181 cgtgtctgcc gcaggggcgc agcaggcgcc ggccccccag atggacgccc agcagatgga
      241 cgcccagcag gtcggggcga gaccggtcgt ggacgcgatg gcaccgtcgg caaagaggtt
      301 gaaggggcag gaaggtctat ggatgcggcc acccgggtcg caggcggcgc ggcggtggcc
      361 ggcctgcgca ccggtccagt actcctcgcc gcggccagct ccagctccaa gcaggcccgg
      421 tgcatgtcgg gacgcgctgc cgggtctttc gccagcaggt tcatcagcac cgcgaacagt
      481 gggcccgcgt gcagcggtgg cggcacctca ccggcggcca ccgcatgcag cagcgcgagc
      541 tggttggtgc cctcaccgaa gggcgcccgc ccctccaccg cggcgtagag agtggcgcca
      601 agcgagaaga cgtccgacgc cgtggtcggg acctgcccgc gagctacctc cggggcgagg
      661 tatgccgggg ttccggccat catcccggtc tgagtgaccg tgacatcccc cgcggcgcgg
      721 gagacaccga agtcggtgat cttcgcaatc ccgtcgtcgg cgatcaggat gtttccgggt
      781 ttgacgtccc ggtgcacgat ctgtgcggcg tgcgcagcgg ccagcgctga cgcgacctgc
      841 gcaccgatcg aggcgacctc atccaccggg agaaagcacc gctcgttcag taccgcagca
      901 aggctgcgtg cgggcaggta ctccatcact aggcagggct cgccgtcgtg ctcggccacg
      961 tcgtagacga cgatggcgtt cgggtgctgc agccgcgcgg cgatgcgcgc ctcacgcagt
     1021 gcccgcagcc gcgcttcctc ggtttcgccg ggactcaaat ccggcgccaa cagcagctgc
     1081 ttcaccgcaa ccagccgttg cagccgctcg tctctggcct gccagacgac acccatgccg
     1141 ccgctgccga tccgatcgag cacccgataa tgcccggcaa tcagttgacc cgtgtcgatg
     1201 agtgacgctc cccgtctgtg ttggcccgtc tgtattggca catctgcact ggccccgacc
     1261 gggggccaag gttaccgcgc ccggcggcgg gacccgcgcc cacggcggcc ccgccgcgcc
     1321 gcccgcgcgg agccgcaggc acgatcgtgt ccatcatgac gcttaccgag gcgctgcccg
     1381 gcggttccgg gcccgacgag gtgctcgaca ccttcgtgaa ctgggtctcg gggcagggct
     1441 tgaccctgta cccggcccag gaggacgcac tgatcgagat cgtctcgggc tcgaacgtga
     1501 tcctggccac ccctaccgga tccggtaaga gcctggtc
//
LOCUS       DATADN010001205         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_752715, whole genome shotgun sequence.
ACCESSION   DATADN010001205 DATADN010000000
VERSION     DATADN010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752715"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VHH53_15440"
     CDS             complement(<1..533)
                     /locus_tag="VHH53_15440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2291540.1"
                     /translation="MDFERPSVARVYDYYLGGSHNFTVDRQVARQAIELWPELPLIMQ
                     TNRAFLRRSVRYLVSQGITQFLDIGSGIPTEGNVHEVAQVASPDARVIYVDIDPVAVA
                     HSRAILAGNERADVVQADLRDVAGILGDPRAKRLIDPAQPLGVLMVAILHFFPDEMDP
                     ASIVAQYRKMMAPGSHFV"
     gene            738..1592
                     /locus_tag="VHH53_15445"
     CDS             738..1592
                     /locus_tag="VHH53_15445"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX2291541.1"
                     /translation="MASEPATNEPASRRAAVLGCPIRHSLSPALHTAAYAGLGLTGWR
                     YEAFECDEASLPAFVGRLGPEWAGLSLTMPLKRVALTVADEITPLASAVGAANTLVFG
                     QPGGRRRAENTDVAGILAALHEAGLDRVGAAVILGAGGTAQAALAALRELGEAAPTVL
                     VRDPVRTGHLRATAERLGVHPTICGGLPDTAVPEADVVISTLPPGAADALAGRQQWSA
                     KPVVLDVVYAPWPTPLAASALHVGCRIVSGLAVLLHQAVAQVQLMTGRNAPIAVMREA
                     LDAAVRFR"
     gene            1700..>1937
                     /locus_tag="VHH53_15450"
     CDS             1700..>1937
                     /locus_tag="VHH53_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HEX2291542.1"
                     /translation="MTVPQPWPGAAYPLGATYDGAGTNFALFSEAADKVELCLFDSGD
                     AGATETRVRLPEVDGFVWHGYLPGVGPGQHYGYRV"
BASE COUNT          330 a          638 c          643 g          326 t
ORIGIN      
        1 acgaaatggc tgccgggcgc catcatcttg cggtactgcg cgacgatact cgccggatcc
       61 atctcatccg ggaagaaatg cagtatcgcc accatcagca cgccgagcgg ctgggccgga
      121 tcgatcagcc gcttggcccg cgggtcaccg aggatccccg ccacgtcgcg cagatcggcc
      181 tgaacgacgt ccgcgcgttc gttcccggcc aggatggccc ggctatgcgc gacggccacc
      241 ggatcgatgt ccacatagat cactcgggca tccggcgaag caacctgtgc tacctcgtgc
      301 acgtttccct cagtagggat gccggagccg atgtccagga attgggtgat gccttggctg
      361 accagatacc gcaccgagcg acgcaggaac gctcggttgg tctgcatgat caagggcagt
      421 tccggccaca gctcgatcgc ctgacgagcc acctgccggt ccacggtgaa gttgtgcgag
      481 ccaccgaggt agtagtcata cactcgggcc acgctggggc gctcaaaatc cacgccaacc
      541 gacgcccagc tcggttgttg caccgcttac ctcctggcgt gatgccgacg accgtttgat
      601 gtgatacagc tgcgcagatc gggctgatct cctcaataac agaccgcaaa cgcgtcatgc
      661 aagaccgcgg ccaacagtct tttcgtcaat actggctaga ggtgaacaaa gggtgcacag
      721 cggggaaaga tttcacagtg gctagtgagc cggcgaccaa tgagccggcc tcgcggcggg
      781 ccgcagtgct gggttgcccg atccggcact cactgtctcc ggcgttgcac accgccgcct
      841 atgcggggtt gggcctgacc ggctggcgct acgaggcgtt cgaatgcgac gaggcgagcc
      901 tgcccgcctt cgtggggcga ctcggaccgg agtgggcggg cctgtcgctg acgatgccgc
      961 tcaaacgggt cgcgttgacc gtggccgacg agatcacccc gctagccagc gcggtcggcg
     1021 cggcgaacac cctggtgttc ggccaaccgg gcggacgccg ccgggctgag aacaccgacg
     1081 tggccggtat ccttgctgcg ctgcacgaag cgggactcga ccgggtcggt gcagcggtga
     1141 tcctcggggc gggtggtacc gcccaagccg cgctcgccgc actgcgcgag ctcggggagg
     1201 cagcgccgac cgttctcgtg cgggatccgg tcagaacagg gcatctgcgc gcgacggctg
     1261 agcggctcgg tgtccatcca acgatttgcg gcggcttacc cgacaccgct gttcccgaag
     1321 ccgacgtcgt catctccaca ttgccacccg gtgccgccga tgcgctggcc ggccggcagc
     1381 agtggtctgc caaacctgtg gtgctcgacg tcgtctacgc gccctggccc acgccgctgg
     1441 ccgcgtccgc actccatgtg ggctgccgga tcgtcagcgg tctggcggta ctgctgcatc
     1501 aggcagtggc tcaggtccag ctgatgaccg gtcgcaatgc cccaatcgcg gtgatgcgcg
     1561 aggcgttgga tgcggccgtg cgattccggt agctatacct gcagctgtca cccaccgcag
     1621 cagctggcac cggtagatca gataccgtga cgcagaaccg gtgcgcggca gttgcccatt
     1681 gacgtggaaa aggagaccga tgaccgtccc ccagccgtgg ccgggtgcgg cctacccgct
     1741 gggtgccacc tacgacggcg caggcaccaa cttcgcgctg ttctccgagg ccgcagacaa
     1801 ggtagaactg tgccttttcg acagcggtga cgctggcgcc accgagacac gggtccggtt
     1861 gcccgaggtc gatgggttcg tctggcacgg ctatctgccc ggtgtggggc caggtcagca
     1921 ctacggatac cgggtgc
//
LOCUS       DATADN010001206         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_753286, whole genome shotgun sequence.
ACCESSION   DATADN010001206 DATADN010000000
VERSION     DATADN010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753286"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VHH53_15455"
     CDS             complement(<1..444)
                     /locus_tag="VHH53_15455"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2291543.1"
                     /translation="MRGGGFGASRRPRSSVIRVVLATLVLLAAMAVLPPAPGRADTGY
                     PVLLVHGGIGSPADFNQMIGWLIADGYRPYTVNLGFPGIDTAANSKKIRARVDQIRAA
                     TGVSKVHLVGHSLGGLSVRYYIKILGGLPHVASYTAFGTPQHGNPD"
     gene            complement(1232..1915)
                     /locus_tag="VHH53_15460"
     CDS             complement(1232..1915)
                     /locus_tag="VHH53_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX2291544.1"
                     /translation="MTVVVAAHGTHDPAGVAVARQLVAALRARLAGRPVLLSFVDVLC
                     PGVREVVTGAPGPVTVVPAFLASGYHVRTDVPREVAATGRGDVTVTAPLGPHQLLVGA
                     MADRLRAGGWHRGDAVVLAAAGSSNWRAVADVRAAAVALSRQVGRRVQVGFIATGAPE
                     VAPLVAGLRATGEPAVAVASWLLSPGLFHGRLAEAGADVVAAPLGLHPGVVDRLAQLA
                     GMAAVLRSA"
     gene            complement(1926..>2293)
                     /locus_tag="VHH53_15465"
     CDS             complement(1926..>2293)
                     /locus_tag="VHH53_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2291545.1"
                     /translation="QPDRSRIGALVRTLAETLPARAPRWPVAGHLLELRGHAAVVDGV
                     LRAVPPTPMALLRLLTARPGRVVPRADLLAVMPAAGDDEHAVETAVARLRAALGAPRL
                     VQTVVKRGYRLALEPGDCR"
BASE COUNT          439 a          788 c          725 g          341 t
ORIGIN      
        1 atcgggattg ccgtgctgtg gcgtgccgaa cgcggtatag ctggccacat ggggtaaccc
       61 acccaaaatt ttgatgtagt agcgcacaga tagtccgccc aggctgtgcc ctaccaggtg
      121 caccttcgag actcccgtcg cggcgcggat ctggtctacc ctggccctga tcttctttga
      181 attggcggcg gtgtcgatcc cggggaagcc gagattgacg gtgtagggcc ggtagccatc
      241 ggcaatgagc cagccaatca tctggttgaa atcggccggg gaaccgatcc cgccgtgtac
      301 gagcagcact gggtagccgg tatcggctcg tcccggcgcg ggtgggagca cggccatcgc
      361 cgcgagcaag acgagcgtgg ccagcaccac gcgtatcacc gagctgcgcg ggcgtcgcga
      421 agcgccgaat cccccaccac gcatgtcagt ccttcctctg atcttgaaag ttggcaggcg
      481 gaccctactc cccgatcgct caccgactcg cctggcttgc ggtagctacg atcaatccgg
      541 cagatcactg acaaggtggg ctgtggcggg ctcggcccac gaccgcttcg ccgacgcagg
      601 tgggcaaggc aatgtgttca gcgcgcccgc cgttgtccac agcgttccga cgaagacgga
      661 tacgagccat ggcccacgaa agcctagaag cacccacagt gacgccaagg accggtgatg
      721 aaccattcac ctatcagggc aagagcattg gagcaggcct tcttcggata ctggcgctag
      781 gcctactcag acttggtgag caacgccacg cgtggtgttc tcgccgagta catcgtcggt
      841 ctggctttaa gatgcataga cggccgcgca cgcgcagagt gggacgcgtc cgatctacgc
      901 acccaggaag gtgattcagt cgaggccaag tcggcagcct atgtgcagac ttggaagcaa
      961 tggaaactat caaaccgtgt tcagcatcat cccccgccaa tgggtggaat gcagcaacca
     1021 acacagtctc gaccgaacgg aaacggcagg gccgatgtat atgtatctgc ctttttagac
     1081 acatggacaa agctaccgtg ttcccgctaa tctggatcag tgggagttct acgttctttc
     1141 cacgaatcga tgtcaagatg gcggccgagg agcccgctaa cgcctcctga accactgccg
     1201 gggcgcgaca gggcacatgc ggtcgccgca gtcacgcgct gcgcagcacc gcagccatcc
     1261 cggcgagttg ggccagtcgg tcgaccaccc ctgggtgcag acccaatggg gcggccacga
     1321 cgtctgcccc ggcttcggcg agcctgccgt gaaacagtcc cggggacagc agccaggagg
     1381 ccaccgccac tgcgggctca ccggtcgctc gcaaccctgc gaccagaggt gccacctccg
     1441 gtgctccggt ggcgatgaac ccgacttgaa cccggcgacc gacctgcctg gacagcgcaa
     1501 ccgccgcggc acgcacgtcg gccaccgctc gccaattgga cgaacccgcg gccgccaaca
     1561 ccaccgcgtc gccgcggtgc cagcctcccg cgcgcaaccg gtctgccatc gcgcccacca
     1621 ggagctggtg cggcccgagg ggcgcagtga cggtcacgtc cccgcggccg gtggcggcca
     1681 cctcccgtgg cacgtcagtc cgtacgtggt aaccgctggc cagaaatgcc gggacgacag
     1741 tgaccggacc cggggcgccg gtgaccactt cgcgcacccc tggacagagc acgtcgacga
     1801 aggaaagcag caccggccta ccagccaacc gggcacgtag cgccgctacc agctgccgcg
     1861 ccacagcgac accggcaggg tcgtgggtgc cgtgcgcggc cacgacgaca gtcacagcaa
     1921 tgccctcatc ggcaatcccc gggttccagg gccagccggt agcctcgctt gactacggtt
     1981 tgcaccagcc gtggagcacc caaagcggca cgcagccggg ccaccgcggt ctccacagca
     2041 tgctcgtcgt caccggccgc gggcatcacg gcgagcaggt cggcgcgcgg taccacccgc
     2101 ccggggcgag ccgtcaacag ccgcagtagt gccatcgggg tcggcggcac ggcgcgcagc
     2161 actccatcga cgaccgccgc gtgcccacgc agctccagca ggtgacccgc taccggccag
     2221 cgtggcgccc gagcaggcag cgtctcggcc aaagtacgca ctagtgcgcc gatccgggag
     2281 cgatccggtt gca
//
LOCUS       DATADN010001207         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_753519, whole genome shotgun sequence.
ACCESSION   DATADN010001207 DATADN010000000
VERSION     DATADN010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753519"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VHH53_15470"
     CDS             <1..658
                     /locus_tag="VHH53_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2291546.1"
                     /translation="GTTGLGYNTKRVPGGKVDSWTALYEPNPALSGRLSILREMTDLI
                     GCTLIYLGKDPNSTKDADLQAVLDTFKGLRRQIKQLRFSSDTYIDELASGEVWLAQGW
                     SGDVFQAQGDNADLAYVIPKEGSLQWADVMCVPKDAPHPENAARFMNYVLQPRVAARI
                     SKYVSYGTPVSLAKALLPPEQLDDPGIYPPSSIKLSFLTLSDQRIGKLRHVYNQIVQG
                     "
     gene            745..1485
                     /locus_tag="VHH53_15475"
     CDS             745..1485
                     /locus_tag="VHH53_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291547.1"
                     /translation="MSTSEPEAAGDPSGGDPPFRVVLWGYDRGPVDVRVAELVRELAD
                     ERRRGDQAEQALSRLRQDIGEGQVQGPEEEAGIAADMAIALQQAGLVAARVLAAAGRQ
                     VEATITAAGLQAADRLKTAAQQASRLEQQARQLLAEAEMERTGIEAAAAREAQQLRAR
                     SDREARAVVAKAREDAELAWREAVRQRQLLQAEAEALATLRQRMLEQLARLLAPLGVV
                     VVDGAGERVLEAAAAPEPRYPAPGLPEA"
     gene            1791..>2383
                     /locus_tag="VHH53_15480"
     CDS             1791..>2383
                     /locus_tag="VHH53_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291548.1"
                     /translation="MTAIDPDSGEADVGAGQFPVVLRGYDRQLVEARLGELAGQLEQE
                     RRQRRNVEQRLDQVDGGNALAVRDQPQASFLSLGARAAKVFEEAGAVAERLIGEAQEQ
                     ARLIIDAAQAEAGERLKAAEDQASALEQMAGERLAEAEATQMRIAAEAAEAAEAVLAD
                     AEHDAQAQRAEAREEIRRAWQKADRERQLLEAEAQRL"
BASE COUNT          435 a          737 c          851 g          360 t
ORIGIN      
        1 gggcacgacc gggctgggct acaacaccaa acgcgttccc ggcggcaagg tcgactcctg
       61 gacggcgctg tatgaaccca acccagccct gagcggccgg ctctccatcc tgcgggagat
      121 gactgatctg atcggctgca cgctgatcta cctcggcaag gatcccaact cgaccaagga
      181 cgccgaccta caagcagtgc tcgacacctt caagggcctg cggcgccaga tcaagcagtt
      241 acggttctcc agcgacacct atatcgacga gctggcctcc ggggaggtct ggctggccca
      301 ggggtggagc ggtgacgtgt tccaggccca aggcgacaac gccgatcttg cctatgtgat
      361 ccccaaggag ggttcgctgc aatgggccga cgtcatgtgt gtgcccaagg acgcgccgca
      421 ccctgagaac gccgctcggt tcatgaacta cgtgctccag cccagggtcg ccgctcgcat
      481 ctccaaatat gtgtcctacg ggaccccggt atcactcgcc aaggccctcc tgccgcccga
      541 acagcttgac gacccgggta tctatccacc gtcatcgatc aagctctcgt tcctcaccct
      601 cagcgaccaa cggatcggaa agttgcggca tgtctacaac cagattgtcc aagggtaatt
      661 cgaatgccgg atcctgtgac cagaaggtcg tgaggtgcag ggctccaaaa ggtgccagcc
      721 aacaggtgag gctgtcggcg cgggatgagc acgagcgaac ctgaggcggc cggcgatccc
      781 agcggcggtg atccaccgtt ccgggtggtg ctctggggct acgaccgggg cccggtggat
      841 gtacgcgtgg ccgagctggt gcgggagctc gccgacgaac ggcggcgagg tgaccaggcc
      901 gaacaagcat tgtcccggct tcgtcaggac atcggcgaag gtcaggtcca aggaccggag
      961 gaggaggcag gcattgccgc agacatggcc atcgcgctcc agcaggctgg actcgtcgcc
     1021 gccagggtgc tcgccgcggc cggcaggcaa gtcgaggcca cgatcacggc cgccggcctg
     1081 caggcggctg accggctcaa gacagccgcc caacaggcga gccgtcttga gcagcaggct
     1141 cggcagctgt tggccgaggc tgagatggag cggactggca ttgaggcggc ggcggcccgc
     1201 gaggcccagc aattgcgggc ccggtcagac cgtgaggcca gggcggtggt cgccaaagcg
     1261 cgggaggacg ctgaacttgc ctggcgggag gcagtgcgcc aacgccagct gctgcaggcc
     1321 gaggccgaag cgctggcaac gctgcgccag cggatgctcg agcagcttgc gcgcctgctt
     1381 gcgccccttg gggtcgtggt ggttgatggc gccggtgagc gcgtgctgga agcggcggcg
     1441 gctccagagc cgaggtaccc tgcgcccgga ctgccggagg cctaatgcgt tggccatatt
     1501 ggtgattcgg tggaggcctc ccatgtcgga tgcgtgggga actctcactc agaccataac
     1561 tgtcgaagct ccaaaagctg tctagacggc tgtgtgcctg ccgccctttg gcgccaatgt
     1621 aacggtagga caagcctgac ggtttcctga ggggcaacgc tccggtctgt ctgtcggtgg
     1681 ccacctcatg aagccccggt gatgccacga agactcggac tgctgtcgcc aacgcgaggt
     1741 cggatgtctg catcgccact ggagcattgc tggaccaaag gaggggcgcc gtgaccgcca
     1801 ttgaccctga ttcgggcgaa gctgatgtcg gtgctggcca attcccggtc gtattgcggg
     1861 gctacgaccg tcagctggtg gaggcgcgct tgggagagct cgccgggcag ctggagcagg
     1921 agcggcggca acgtcgaaac gttgagcaga ggctggacca ggtcgacggg ggcaacgcct
     1981 tggcggtccg cgaccagccg caagcctcgt tcctcagcct gggggcgaga gcggccaagg
     2041 tcttcgagga ggccggagcc gtcgccgaac gcctgatcgg tgaggcacag gagcaggcgc
     2101 ggctgatcat cgacgcagcc caggctgagg ctggcgagcg gctcaaggcg gccgaggacc
     2161 aggcgagtgc gcttgagcag atggccggcg agaggttggc cgaggccgag gccacgcaga
     2221 tgcggatagc ggccgaggcg gccgaggcgg ccgaggcggt cttggccgat gccgagcacg
     2281 atgcccaggc acaacgagcc gaggcccgcg aggagattcg gcgtgcctgg cagaaagccg
     2341 accgcgagcg ccagctcctt gaggcggagg cgcagcggtt gca
//
LOCUS       DATADN010001208         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1345558, whole genome shotgun sequence.
ACCESSION   DATADN010001208 DATADN010000000
VERSION     DATADN010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345558"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..995)
                     /locus_tag="VHH53_15485"
     CDS             complement(21..995)
                     /locus_tag="VHH53_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HEX2291549.1"
                     /translation="MGSVDDRDLLPMRSTFDIVRRGYDCAQVDYQLAQLQADLQIATA
                     DRDAGAAQAGQLARQLEAARAELDDLRIQVQRLAAPPTTVEGMSERVARMLRKAQEEA
                     AEIIAKGTAVAAATRANAERKADELRGRYEQMIGEAQRRRSAMEAEHERVIADARRRG
                     DEILAKAKQRAEQRDAESTARRQQAEEDFDITMSTRRRDAMRAMAEEEASSKSEAQRR
                     IDEATDVARRLVTEADQTAVARVQEATRQVEQLRTLRRRLIDQLQSVRSKLDDAFQQL
                     APAPEEDSVLAGAQARSERAAASPMQASGSPAAAGSPAAGVPVPPVSQ"
     gene            complement(1043..>1596)
                     /locus_tag="VHH53_15490"
     CDS             complement(1043..>1596)
                     /locus_tag="VHH53_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2291550.1"
                     /translation="EICRRMGAELVIDRSAEGYRFWKDEHNQDPKEWKRFGGRIRELT
                     GGDDPDIVFEHPGRETFGASVYVAKRGGTIVSCASTSGYNHTYDNRYLWMNLKRIIGS
                     HFANYREAWEANRLITKAKVHPTLSRVYPLDETGQAARDVHCNIHQGKVGVLCLAPEE
                     GLGVRNPELREAHLAGINRFRGV"
BASE COUNT          224 a          552 c          524 g          296 t
ORIGIN      
        1 ggccggacgg ggagcaggga tcattggctg accggcggca ctggcacacc agctgctggg
       61 ctgcccgccg ctgccgggct cccactcgcc tgcattggtg aggcggcagc ccgttcagag
      121 cgcgcttggg caccggcgag cacgctgtcc tcctctgggg cgggagccag ctgctggaag
      181 gcgtcatcga gcttcgaacg caccgactgc agctgatcga tgagtcgacg gcgcagcgtg
      241 cggagttgct ccacctgccg ggtagcctcc tgcaccctgg cgacagcggt ctggtcggcc
      301 tcggtgacca ggcgccgggc cacgtcggtc gcctcgtcga tgcggcgctg cgcctccgac
      361 ttgctgctcg cctcctcctc ggccatcgcg cgcatcgcgt cgcggcggcg ggttgacatg
      421 gtgatgtcga agtcctcctc ggcctgctgc cggcgggccg tcgactcggc atcgcgctgc
      481 tcggctcgct gcttggcctt tgccaggatc tcgtcacccc gtcgacgagc gtcggcgatg
      541 acccgctcgt gctcggcctc catcgccgat cgccgccgct gcgcttcacc gatcatctgt
      601 tcgtagcggc cgcgcagctc gtcggccttc cgctcggcgt tggccctggt cgccgccgca
      661 acggcggtgc ccttggcgat gatctccgcg gcctcctcct gagccttgcg gagcatccga
      721 gccacgcgct cactcatgcc ctcgacagtg gtcggtgggg cggcgagccg ctgcacctga
      781 atgcgtaagt catcgagttc ggcgcgtgca gcctccagct gccgggcgag ctgcccagcc
      841 tgcgcagcgc cggcatcccg gtccgctgtc gcgatctgca ggtcggcctg cagctgcgcg
      901 agctggtagt ccacttgggc gcagtcgtaa ccgcggcgca cgatgtcgaa cgtagatctc
      961 atcgggagca gatcacggtc gtcgactgag cccataacgt cagattactc gggtacgagt
     1021 taccggagat gagccgttcc ggtcagaccc cacggaagcg attgatgccg gccagatggg
     1081 cctcgcgaag ctcgggatta cgtacgccca ggccctcctc cggggccaag cacagcaccc
     1141 caaccttgcc ttggtggatg ttgcagtgca catcccgggc agcttgcccg gtctcatcca
     1201 gtggatagac ccgtgacagc gtcggatgaa ccttggcttt ggtgatcagc cggttggcct
     1261 cccaggcctc gcgatagttg gcgaaatgcg agccgataat ccgtttgagg ttcatccaga
     1321 ggtagcggtt gtcgtaggtg tggttgtacc cggaagtcga ggcgcagctg acgatcgtgc
     1381 cgcccctctt ggcgacgtag acgctggcgc cgaaggtctc ccgccccggg tgctcgaaca
     1441 cgatgtcggg gtcgtcaccg ccagtgagct cgcgaatccg cccgccgaaa cgcttccact
     1501 ccttcggatc ctggttgtgc tcgtccttcc agaaccggta gccctcggca ctgcggtcga
     1561 tgaccaactc ggcacccatc cgccggcaga tctcgg
//
LOCUS       DATADN010001209         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_753723, whole genome shotgun sequence.
ACCESSION   DATADN010001209 DATADN010000000
VERSION     DATADN010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753723"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1611)
                     /locus_tag="VHH53_15495"
     CDS             complement(<1..>1611)
                     /locus_tag="VHH53_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HEX2291551.1"
                     /translation="LPLGTIPVSPANCVIKISSDATGTTASVGPHQFVTVDQDVRPQV
                     TGIYSALDDQRDQVKGLAVQITPDTRFQSTPHPIKLAAMTLAVVAVVTSLMLLHRLDG
                     RIGRRAPRLLPSGWWRPTGRDATVLAVLAVWVVIGGITSDDGYILTMIRTSSEMGYVG
                     NYYRWLNVPEAPFGWPYELYMLWAEISTTPPWLRLPSFVMGGVSWMLISREVLPRLGR
                     EVRRSAAAGWAAAAVFLAFWLPYNNGLRLEPVVAIGSLLALCAVERAVATRRLLPLAL
                     GLLAAAFTVAATPTGFIAVAPFLVAVRPLVRLLHQHASVSGWPAVIGPIFGGGLLVLV
                     VIFADQTLAGLLEATRIRTAIGPSLSWFQEATRYQELFSDKADGALARRFPVLLLLLC
                     LGTSLVVLLRRGSIPGAGLGPSRRLIATAALALALLALTPTKWTHHFGSLASVGAALA
                     ALTALATSTSVLRSARNRWWFLSGLLVILALSATGPNAPWYVSQYGVPWYDIPPSVNG
                     FQASTLLIIAAALAGLLAVIEGLRHEPGA"
BASE COUNT          290 a          547 c          567 g          207 t
ORIGIN      
        1 gcacccggct catgccgcaa tccctcgatc acggcgagca gcccggccag cgcggcggcg
       61 atgatcagca gcgtgcttgc ctggaagccg tttaccgacg gggggatgtc gtaccagggc
      121 accccgtact gggagacata ccagggtgcg ttgggcccgg tagccgacaa cgccaggatt
      181 accagcaggc cggacaggaa ccaccaccgg ttgcgcgccg aacgtagcac gctggtgctg
      241 gtggccagtg cggtcagcgc ggcgagcgcg gcaccgaccg aggcgagcga cccgaagtgg
      301 tgggtccact tggtcggagt cagtgccagc agcgccagcg ccagcgccgc ggtggcgatc
      361 agtcgccggc ttggacccag gccggcgccc gggatgctgc cccggcgcaa cagcaccacc
      421 aggctggttc ccaggcatag cagcaacagc agcaccggga agcggcgggc cagcgccccg
      481 tcagccttgt cgctgaacag ctcctggtag cgagtggcct cttggaacca ggacaggctt
      541 ggtccgatcg cggtgcgaat ccgggtggct tccagcagcc cagccagtgt ctggtcggca
      601 aagatcacca cgaggaccag caggccgcca ccgaagatcg gtccgatcac cgcgggccag
      661 ccggagacgg atgcgtgctg gtgcagcaac cgcaccagcg gccgcaccgc caccaggaac
      721 ggtgcgaccg cgatgaatcc ggtcggcgtt gcggcgacgg tgaaggcggc ggcgagcagg
      781 cctagcgcca gcggcaacag ccggcgggtg gccaccgcac gctccaccgc acacagcgcc
      841 agcagcgagc cgatcgcgac gaccggctcc agccgcagcc cattgttgta gggcagccag
      901 aaggccagga acaccgcggc ggcggcccat ccggcggcgg cactgcggcg gacctcccgg
      961 cccaaccgcg gcagcacctc ccggctgatc agcatccagc tgaccccacc catgacgaag
     1021 gagggcagcc gcagccacgg tggggtcgtg ctgatctccg cccataacat gtacagctcg
     1081 tagggccagc cgaagggcgc ctcggggacg ttcaaccatc ggtagtaatt gccgacgtag
     1141 cccatctcgg agctggtccg aatcatcgtg aggatgtagc cgtcgtcaga ggtgatccct
     1201 ccgatcacga cccataccgc cagcacggcg agcaccgtgg cgtcgcgtcc ggtaggccgc
     1261 caccagcccg acggcagcag ccggggcgct cggcggccga tccgcccgtc cagccgatgc
     1321 agcagcatca ggctggtcac cacggctacc accgcgagcg tcatcgcggc gagcttgatc
     1381 ggatgtgggg tggactgaaa gcgggtgtcc ggggtgatct gtaccgccag ccccttgacc
     1441 tggtcacgct ggtcgtcgag ggccgagtag ataccggtca cctgcggccg gacgtcctgg
     1501 tcgactgtga cgaactggtg cgggccgacc gaagccgtgg tgccggtggc gtcggaggag
     1561 attttgatca cgcagttggc cggggacacc gggatggtgc ccaacgggag g
//
LOCUS       DATADN010001210         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_136094, whole genome shotgun sequence.
ACCESSION   DATADN010001210 DATADN010000000
VERSION     DATADN010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136094"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VHH53_15500"
     CDS             <1..1283
                     /locus_tag="VHH53_15500"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2291552.1"
                     /translation="LHVVIRFLAPYADPVLLPAVALLNGLGVGFLRRLDLARAIAEDQ
                     PVPGIFAGTGGRQLAWTLASLILAAVLLVVIRDHKIVSRYAYTLGLVGIVLVMIPAVL
                     PSSISEVNGAKLWIRIGSFSIQPGEFAKLALVSFFAYYLVRKREVLSLASKRFLGIDF
                     PRGRDLGPVVTVWILSLLVLVFEKDLGTALLYFGLFIVTVYIATERVSWLIIGLLLFF
                     GGVYVAYLLGQTVGGPFANFYDRAEVWLDPFAADQYERQGGSYQLVQSLLGLGTGGLF
                     GTGPGAGSPGFVPEVRTDFIFAGMGEEIGLFGLSALLVMYLLIVERGLRAAIAVRDSF
                     GKLVAGGLAFTLGLQVFVIVGGITGLIPLTGQTTPFLSAGGSSLMANWLLVAMLLRIS
                     DAGRRPASGGPERAPGPKQPPAQLHGAPTEVIKP"
     gene            1280..>1589
                     /locus_tag="VHH53_15505"
     CDS             1280..>1589
                     /locus_tag="VHH53_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX2291553.1"
                     /translation="MNAPLRKAGVVILVLFGLLFANLNWVQGYKADDYRTSDWNRRVT
                     VAEYERPRGIIEAGGEPLANNRATEGTLKYLRVYPEKELYAPIIGYKPVNNAETGIEK
                     S"
BASE COUNT          208 a          536 c          558 g          287 t
ORIGIN      
        1 ccctgcacgt ggtgatccgg ttcctcgcac cgtacgccga cccggtgctg ctgccggcgg
       61 tcgcgttgct caacggcctc ggcgtgggct tcctgcgccg cctggacctg gcccgggcga
      121 tcgccgagga ccagcccgta cccgggatct tcgccgggac cggcggccgg cagctggcct
      181 ggacgctggc ctccctgatc ctggccgcgg tgctgctcgt ggtgatccgg gaccacaaga
      241 tcgtttcccg gtacgcgtac acgctcggtc tcgtcggcat cgtgctggtg atgatcccgg
      301 ccgtgctgcc gtccagcatc tccgaggtca acggcgcgaa gctgtggatc cggatcggtt
      361 ccttctcgat ccagccgggc gagttcgcga agctcgcgct ggtgtccttc ttcgcctact
      421 acctggtccg caagcgtgag gtgctgtcgc tggccagcaa gcggttcctc ggcatcgact
      481 tccctcgcgg gcgggacctc ggcccggtcg tcacggtctg gatcctcagt ctgctggtgc
      541 tggtcttcga gaaggacctc ggtaccgcgc tgctgtactt cggcctgttc atcgtcacgg
      601 tgtacatcgc caccgagcgg gtcagctggc tgatcatcgg cctgctgctg ttcttcggcg
      661 gcgtctacgt ggcgtacctg ctgggccaga ccgtgggcgg cccgttcgcc aacttctacg
      721 accgcgccga ggtctggctc gacccgttcg ccgccgacca gtacgaacgc cagggcggca
      781 gctaccagct ggtgcagagc ctgctcgggc tgggcaccgg cgggctgttc ggcaccgggc
      841 cgggcgccgg ctcgccgggc ttcgtacccg aggtgcgcac cgacttcatc ttcgccggca
      901 tgggcgagga gatcggcctg ttcgggctgt ccgcgctgct ggtgatgtac ctgctgatcg
      961 tcgagcgcgg cctgcgcgcg gcgatcgcgg tgcgcgactc gttcggcaag ctggtcgccg
     1021 gtggcctcgc gttcaccctg ggtttgcagg tcttcgtcat cgtgggtggc atcaccggac
     1081 tgatcccgct gaccgggcag accacaccgt tcctgtccgc gggtggttcg tcgctgatgg
     1141 cgaactggtt gctggtcgcg atgctgctgc ggatctcgga cgcggggcgg cggccggctt
     1201 ccggcgggcc cgagcgggca cccggaccga aacaaccgcc ggcccagttg cacggcgccc
     1261 cgacggaggt gatcaagccg tgaacgcacc gttgcggaag gccggcgtgg tcatcctcgt
     1321 gctgttcggg ctgctgttcg ccaacctcaa ctgggtgcag gggtacaagg ccgacgacta
     1381 ccggaccagt gactggaacc gccgcgtgac ggtggccgag tacgagcgcc cgcgcggcat
     1441 catcgaggcc ggcggcgagc cgctggccaa caaccgggcc accgagggca cgctgaagta
     1501 cctgcgggtc tatccggaga aggagctgta cgcgccgatc atcgggtaca agccggtgaa
     1561 caacgcggag accggcatcg agaagagcg
//
LOCUS       DATADN010001211         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_293496, whole genome shotgun sequence.
ACCESSION   DATADN010001211 DATADN010000000
VERSION     DATADN010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293496"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1546)
                     /locus_tag="VHH53_15510"
     CDS             complement(272..1546)
                     /locus_tag="VHH53_15510"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX2291554.1"
                     /translation="MTLHHPGGEHQMSVTRATLGASGIDLGTLRANTGMVTLDPGFVN
                     TAACTSEITYIDGDAGVLRYRGYPIGQLAEHSTYLETSYLLIYGDLPTSGQLEHFTDQ
                     IRMHTLLHEDLKRFFDGFPRDAHPMPVLSSAVSALSTFYQDSLDPFDQEQVEISTVRL
                     LAKLPTIAAYAYKKSVGQPFLYPDNSLSLVHNFLRMTFGLPAEPYEVDPVVADALDLL
                     FILHADHEQNCSTSTVRMVGSSQANLFASVSAGINALFGPLHGGANQLVLEMLERIHL
                     DGGDVTAFVKKVKNHSDGVRLTGFGHRVYKNYDPRAKIVKKTADEILHKLGVNDPLLD
                     IALALEEQALADDYFVERKLYPNVDFYTGLIYRAMGFPTRMFTVLFALGRLPGWIAHW
                     REMMSDSETKIGRPRQVYTGRTEREYTTITQR"
     gene            complement(1788..>2726)
                     /locus_tag="VHH53_15515"
     CDS             complement(1788..>2726)
                     /locus_tag="VHH53_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2291555.1"
                     /translation="LALVNFRGVGESVKANLVLTCIELAGLLIIIMIGVYAVGSGQGE
                     PSRLAQIDTSDSGVLLAITSATALAFFAMVGFEDSVNMAEETKQPAKIFPRAMLLGMA
                     AAGVIYVLVAVTSSLLVPADRLATLESGALLEVVRVGAPGFPLGLFAIIGLFAVINSA
                     LINMLMASRLVYGMAGERIIPRALGSVHPTRRTPWVSILFTSAIAVVLVSFVPLTGLA
                     GTTSLLLLAVFTLVNVACLVLRRKPVEHVHFRAPSWSPWLGALLCGYLATPLSGRDPG
                     QYLLAGVLLAVGLLLWLLNRAFVGRVEFDPAKLSRE"
BASE COUNT          527 a          843 c          891 g          465 t
ORIGIN      
        1 gccgcctgct ggtcctgcat gaagctccca tcgaacgccg cgccttggtg gccgccgtca
       61 gtggcaacca ccgcgaacga cctggctgac agtgtgcagc gccactcgtc gctgttgagg
      121 tagggcggtc tggtcgatca catcgacgtg tccgtcgacc cattggatgg tgcgcatgta
      181 ttccggctcc cggggttcgg cctgcgggca ccgtacgcgg tacgcaggga gtcgcgcacc
      241 gcccaacggg cgatcccaca ctcggtagag atcaacgctg ggtaatcgtg gtgtactccc
      301 gctcggtgcg tccggtgtaa acctgccgcg gcctaccgat cttggtttcc gagtcgctca
      361 tcatctcgcg ccagtgcgcg atccaacccg gtagccggcc cagcgcgaag agcaccgtga
      421 acatccgggt gggaaagccc atcgcccggt agatcagacc ggtgtagaag tcgacgttgg
      481 gatagagctt gcgctcgacg aagtagtcgt cggcgagcgc ctgctcctcc agtgccaggg
      541 cgatgtccag cagagggtcg ttgaccccga gtttgtgcaa gatctcgtcc gcagttttct
      601 tgacgatctt cgcacgcgga tcgtagtttt tgtagacccg atgcccgaag cccgtcagcc
      661 gcacaccgtc ggagtggttt ttcaccttct tgacgaaggc ggttacgtca cccccatcca
      721 ggtggatgcg ttcaagcatc tccagcacca actggttggc cccgccgtgc agcgggccga
      781 acagcgcatt gatccccgcc gagaccgagg cgaacaggtt ggcctgtgag gagccgacca
      841 tccgtacggt ggaggtcgag cagttctgct cgtgatcggc gtgcaggata aacaacaggt
      901 ccagcgcgtc ggccaccacg gggtcgacct cgtagggctc cgcgggaagc ccgaaggtca
      961 tccgcaggaa gttgtgcacc aggctcagcg agttgtccgg ataaaggaac ggctggccga
     1021 ccgacttctt gtaggcgtaa gccgcgatag tgggtagctt ggccagtagc cgcactgtgg
     1081 aaatctccac ttgttcctgg tcgaacggat cgaggctgtc ctggtagaag gtggacagcg
     1141 cggacaccgc gctggacagc accggcatcg gatgggcgtc gcgcgggaag ccgtcgaaga
     1201 aacgtttgag gtcctcgtgc aacagcgtgt gcatgcggat ttggtcggtg aagtgctcca
     1261 attgaccgct ggtcggcagg tccccgtaga tcagcaggta gctggtctcc aggtaggtcg
     1321 agtgctctgc gagctggccg atcgggtagc cgcggtagcg cagcacgccc gcgtcgccgt
     1381 cgatgtaggt gatttctgat gtgcaggcgg cggtgttcac gaagcctgga tcgagggtga
     1441 ccatcccagt gttggcccgc agcgtgccca ggtcgatccc ggacgccccc agggtggccc
     1501 tggtgaccga catctgatgt tcgccgcctg ggtgatggag ggtcacactg tcataatcat
     1561 tatcagcacg atcattgttg gattcattac gaccgttgct ggccggtcga accgacactt
     1621 cggacgcggt ggcgtcagtc atggcagtcc ctctcagttc tcaggcgaca cttcgtgggc
     1681 ggcgcagcgc aacgctgcgg tagccggtcg ggcggcacag cggtgatgca tggagcgacg
     1741 ttgcatgaag gcacatcgtc gggaacgccg acatactcca aagtaactca ctcgcgggaa
     1801 agcttcgccg ggtcgaactc gacccgaccc acgaacgccc gattgagtag ccagagcagg
     1861 agcccgaccg ccagcaggac gcctgcgagt aggtactggc cagggtcgcg acccgacaac
     1921 ggggttgcca ggtaaccgca cagcagcgca ccgagccacg ggctccagga cggggctcgg
     1981 aagtgcacgt gctcgacagg cttgcggcgc agcaccaggc aggcgacgtt gaccaaggtg
     2041 aataccgcca gcagcagcag cgaggtagtc cccgccagac cggtcagcgg gacgaaactg
     2101 accagaacca cggcgatcgc gctggtaaac aagatcgaca cccacggggt tcgccgggtc
     2161 gggtgcaccg agcccagcgc ccgcggaatg atccgctcgc ccgccatgcc gtagaccagc
     2221 ctgctggcca tcagcatgtt gatcagagct gagttgatca ccgcaaagag cccgatgatg
     2281 gcgaagaggc ccaacgggaa gccgggggca ccgacccgga caacctcgag cagcgcacca
     2341 ctttccaggg tggccagccg atcggcgggt accagcagcg aggaggtgac tgcgaccagc
     2401 acgtagatca ccccggcggc ggccatgccg agcagcatcg cgcgcggaaa gatcttggcc
     2461 ggctgtttgg tctcctcggc catgttcacc gagtcctcga aaccgaccat cgcgaagaac
     2521 gccagcgcgg tggccgaagt gatcgccagc agcacacccg aatccgaggt gtcgatctgg
     2581 gcgagcctgc tcggctcgcc ttgacctgag ccgaccgcat acacgccgat catgatgatg
     2641 atcagaagtc ccgccagctc gatgcaggtg agcacgagat tggccttgac tgactcaccg
     2701 acgccccgga aattgaccag tgcgag
//
LOCUS       DATADN010001212         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_894731, whole genome shotgun sequence.
ACCESSION   DATADN010001212 DATADN010000000
VERSION     DATADN010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894731"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..929
                     /gene="argB"
                     /locus_tag="VHH53_15520"
     CDS             36..929
                     /gene="argB"
                     /locus_tag="VHH53_15520"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX2291556.1"
                     /translation="MTTKIDAAAQKAAVLTEALPWLQRFHGTTVVIKYGGSAMLADEL
                     KHAFVQDMVFLRLAGLRPVVVHGGGPQISAMLDRLGIAGEFRGGLRVTTEETIDVVRM
                     VLFGQVGRELVGMINAHGPFAVGLSGEDAQLFTAVRRTATVNGEQIDVGLVGDVVAVN
                     PAAVLDIVNAGRIPVVSGLAPDSDGVVHNINADTAAAALAIALGAEKLVMLTDVEGLY
                     TDWPDQSSLLSQVTVDRLETLLPRLSAGMAPKMEACLRAVRGGVPRAHVIDGRVAHSV
                     LLEVFTTEGVGTMVLPGEGHD"
     gene            929..>1946
                     /locus_tag="VHH53_15525"
     CDS             929..>1946
                     /locus_tag="VHH53_15525"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX2291557.1"
                     /translation="MGTADLQRRWEAALMANYGTPALALARGAGAEVWDAGGRRYLDL
                     LGGVAVNVLGHAHPAIVRAVTEQISTLGQVSNLYLSEPAITLAETLLDLLGATGSGRV
                     LFCNSGAEAIEAAFKIARRTGRRKMVAAYGAFHGRTMGALALTGQPAKRDPFEPMPPG
                     VVFVPYGDPAALAEVVDEDTAAVFLEPVQGENGVVIPPEGYLHAAREITTARGALLVL
                     DEVQTGIGRTGTWFAHQSLGVAPDVVTLAKGLGGGLPIGACIGIGPAAHLLQPGQHGT
                     TLGGNPVCCAAAQAVLATIASDGLLDHAARLGKEVAAGIAAMSHPLVGPISEAGLLIG
                     IGLTS"
BASE COUNT          291 a          635 c          705 g          315 t
ORIGIN      
        1 cacggatacg tcgacgagaa cagcgcgtac tcgtcatgac gacgaagatc gatgcagcgg
       61 cacagaaagc cgcggtgctc accgaagcgc tgccctggct gcagcgcttc catgggacca
      121 ccgtcgtgat caagtatggc ggtagcgcga tgctggcgga tgaactcaag cacgcgtttg
      181 tccaggacat ggttttcctg cggctcgccg ggctacgccc agtcgtggtg cacggcggcg
      241 ggccccagat cagcgccatg ctggatcgcc tgggcatcgc gggggagttt cgcggaggat
      301 tgcgggtcac cactgaagag acgatcgacg tagtgcgcat ggtgttgttc ggtcaggtgg
      361 gccgcgagct cgtcgggatg atcaacgcgc acggtccgtt cgctgtcggc ctctccggcg
      421 aggacgcgca gctgttcacc gccgtccggc gtaccgctac cgtgaacggc gagcagatcg
      481 acgtggggct ggtcggcgat gtggtggcgg tgaaccccgc cgcggtgctc gacatcgtca
      541 acgccgggcg catccccgtg gtgtccggac tggccccgga ctccgacggc gtggtgcaca
      601 acatcaacgc tgacaccgct gcggcagcgc tggccatagc gctgggcgcc gagaagctcg
      661 tcatgctcac cgacgtcgag ggcctctaca ccgactggcc ggaccagtcc agcctgctgt
      721 cccaggtgac cgtcgatcgc ctggagacgc tgctgccgcg gctgtccgcg gggatggcac
      781 cgaagatgga ggcctgcctg cgcgcggtac gggggggagt gccacgggcc catgtcatcg
      841 atggccgggt ggcgcattcg gttcttctag aggtgttcac caccgagggg gtagggacga
      901 tggtcctgcc cggggagggt cacgactgat gggtaccgcg gaccttcagc ggcgctggga
      961 agccgcgttg atggccaact atgggacgcc cgcgctggcg ttggcccgag gtgcaggggc
     1021 ggaagtgtgg gacgccggcg gccggcgcta cctggatctg cttggcggtg tcgcggtgaa
     1081 tgtgctcggc cacgcgcacc cggcgattgt gcgcgccgtc accgagcaga tcagcaccct
     1141 cgggcaggtg tccaacctgt acctcagcga gccggcgatc acgctggccg agactctgct
     1201 cgacctgctc ggtgcgaccg gatcggggcg ggtgctgttc tgcaactccg gtgcagaagc
     1261 catcgaggcc gcattcaaga tcgctcggcg caccgggcgg cggaaaatgg tcgccgccta
     1321 cggcgccttt catggccgga ctatgggcgc gctcgcgctc accgggcagc ccgccaagcg
     1381 ggatcccttc gagccgatgc cccccggcgt ggtgttcgtg ccttacggag accctgcggc
     1441 gctggcggag gtggtcgacg aggacaccgc cgcggtgttc ctcgagcccg tccagggcga
     1501 gaacggcgtg gtgatccccc ccgagggtta cctgcacgcc gctcgggaga tcaccactgc
     1561 gcgcggggcc ctgctggtgc tcgacgaggt gcagacgggc atcggccgga ccggcacctg
     1621 gttcgcccac cagtccctgg gggtggcccc cgacgtggtg acgctcgcca agggtctcgg
     1681 cggggggctg ccgatcggtg catgtatcgg catcggaccc gccgcgcatc tactacaacc
     1741 aggccagcac ggcaccaccc tcggtggcaa cccggtgtgc tgcgcggccg cacaggccgt
     1801 gctggcgacc atcgcctcgg atggcttgct cgatcatgct gcccgactcg gcaaggaggt
     1861 cgccgccggg atcgcggcga tgagccaccc gctcgtcggg ccgatcagtg aggcgggact
     1921 gcttatcggc atcggtctca cgtcgc
//
LOCUS       DATADN010001213         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1346540, whole genome shotgun sequence.
ACCESSION   DATADN010001213 DATADN010000000
VERSION     DATADN010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346540"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..674)
                     /locus_tag="VHH53_15530"
     CDS             complement(36..674)
                     /locus_tag="VHH53_15530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2291558.1"
                     /translation="MIERLLHDGTVVARPDGRVHRLFPVAVGAVEGEALRDWVVREGA
                     TRTIEIGLGYGISALFICEGLLSNGDAAARHVVVDPYQATRFRNCGLQVVEEAGVASL
                     VEHHALESQIALPRFLSEGRRFDLAFVDGNHRFDGIFLDLVYLGRLLHPGAIVLVDDY
                     QLPAVARAVSFCLTNLGWRLEEVSPVDELHQWAVLRTSAAPDTRPFDYYVEF"
     gene            complement(1070..1267)
                     /locus_tag="VHH53_15535"
     CDS             complement(1070..1267)
                     /locus_tag="VHH53_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291559.1"
                     /translation="MLTHDRHETDSLLAPGERLTPWMIHDLPRIAWIVEYRAAELTDE
                     ERQLVRDATTRMVRALEHDTG"
BASE COUNT          280 a          599 c          555 g          250 t
ORIGIN      
        1 gccctcggcc ggcagacccg gcggaccgca gggcatcaga actcgacgta gtagtcgaac
       61 ggtcgtgtgt ccggagccgc agaggtgcgc aggaccgccc actggtggag ctcatcgaca
      121 ggcgacacct cctcgagtct ccagccgaga ttcgtcaagc agaacgacac cgcacgggcg
      181 accgccggca gctgatagtc gtccacgagc acgattgcgc ccgggtgaag caggcgtccc
      241 aggtagacaa gatccaggaa gatcccatcg aagcggtggt tgccgtccac gaaggcaaga
      301 tcaaagcgcc gcccctcgct gaggaaccgc gggagtgcga tctgcgactc cagcgcgtgg
      361 tgctccacca gcgacgccac gccagcttcc tccacgacct gcagcccaca gttccgaaac
      421 ctcgttgcct ggtagggatc aaccaccaca tgtctcgcgg ccgcgtcgcc gttgctgagg
      481 aggccctcgc agatgaacag cgccgagatg ccatagccaa ggccgatctc gatggtccgc
      541 gtggcgcctt cacgcacgac ccagtcgcgc agcgcctcac cctcgacagc gccaacggcg
      601 accgggaaca ggcggtgcac tctgccatcc ggccgcgcga ccaccgtgcc gtcatggagt
      661 agccgctcga tcacctgccg tacctgtcgc agcgccgcgc ggtgatccga cgggttggcg
      721 ggatccaccc gagcagggtc acacggccag gttggccagg caagccggca ggcgccaggc
      781 gcagctgccg cgcctccgag gagcttgcca cgaggcaggc ggtcgccgag gacgggcctc
      841 gccctccggt cgagctggtg ccatcaccgc caggctccga gccgcgtcct cctcgccgcc
      901 gtgccccgac gtgccctaag catcggtcgg catcggtcat ccacggacaa ccacggtcca
      961 tgcccatgcc ctgcgagcag taggatcatc ccgcacagag cgggccgagg gtgcttccca
     1021 gctcgcggtt ccttcaacac agggtcagtc tgctgggatg gcgaccagtt cagccggtgt
     1081 catgctccag ggcacgcacc atccgcgtgg tcgcatcgcg cacgagctgc cgctcctcgt
     1141 cggtcaactc tgccgcgcga tactccacaa tccaggcgat cctcggcagg tcatggatca
     1201 tccatggcgt gagccgctcg ccaggcgcca gcagcgaatc cgtctcgtgc ctgtcgtggg
     1261 tgagcacgct gccgcaggtc gggcacctcg gggcaggcgt tgtcatgcat gggagtatgg
     1321 gccgcgctga ggcgccggtc cacctggtcc gcactgggtc cgcatggcat cgggaaccgc
     1381 tggtcgtcgg cgggcacgag cggtcgcggc ggcaggaaga agccgcaggc caccacgcct
     1441 tcccgcccac aaccacggcc tcttacgtct ggtcgagagg attccgctgc ctgtcgtacg
     1501 ggatgcccgc gcgacggcag ttatcaggag atccacttcc tggccgtcgc atcagcgccg
     1561 tcggatgggc aggtggccgg tgcgactcat ccgactagct gcggtgccgg cgttgcgccc
     1621 tacgtccccg cgctgcggac gaggtcggtc aggctcaccc gtgctcggtc ccgggctggg
     1681 gggg
//
LOCUS       DATADN010001214         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_293610, whole genome shotgun sequence.
ACCESSION   DATADN010001214 DATADN010000000
VERSION     DATADN010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293610"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VHH53_15540"
     CDS             complement(<1..402)
                     /locus_tag="VHH53_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2291560.1"
                     /translation="MTESAQLESSVNGHLPQNGDRPQLSLATDAARNLATTTKSQPQM
                     QEITSRWLLRVLPWVEASGGTFRVNRRASYAVGDGRMTFTMNGDAETRVIPDELRELP
                     MLRRFTEHPDVLSEIADQFEQRDIDAGSEIVS"
     gene            complement(565..>1507)
                     /locus_tag="VHH53_15545"
     CDS             complement(565..>1507)
                     /locus_tag="VHH53_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2291561.1"
                     /translation="AGDLAMVAAHARQAQGHHPADRFRLPVRLAARAAMAAAERGHLV
                     EARRHLDAMTRANGRPLGILEPWYWWAEATVARAEGRLAAAASVLERAVDGYSVMNVW
                     ALRAFVLADLAEIAVVIGDPGGAARAATSAEDNARRTGAPIHQALHQFATAWALICRG
                     RHDLAARAALGAMDGFDSSGYALLAARARVAYANAVRRSDRGAAQEALRAATAAFDAC
                     GAVVRRDQARALLRQLESQGRCTAQTPGPGSLTPRERQVAELASGGYTAPQIATRLHI
                     GVRTVETHLARSYLKLGVTSKQQLVAHGAELGLAAGR"
BASE COUNT          233 a          531 c          487 g          256 t
ORIGIN      
        1 gctgacgatc tcgcttccgg cgtcgatgtc gcgctgctcg aactggtccg ctatttcact
       61 cagtacgtcg ggatgctccg tgaagcgccg cagcatcggc aactcgcgca gttcatcggg
      121 gataacccgt gtctcggcgt cgccgttcat cgtgaacgtc atccggccat cacctaccgc
      181 atagctggcc cggcggttca cccggaacgt cccacccgat gcttctaccc acggcaacac
      241 tcgcaacagc cagcgcgacg tgatttcctg catctgcggc tgcgacttgg tcgtcgttgc
      301 cagattacgg gcagcgtcgg tagccaggct cagctgtggc cgatcaccgt tttgcggcag
      361 atgaccgttt acgctcgact ccaactgcgc cgattcagtc acaacacaca ccaccaatct
      421 ctatccgcac tgcatcttta tccggactgg gaggaaggat aaggtcgtcc ctacctgtgg
      481 cgaccggctg ttgccgatac caacatcggg actcactgta gggatttcgg gttccgtggt
      541 agtccgtaga aactacggat ttcgctaccg gccggctgct aacccgagct cggcgccatg
      601 ggccacgagt tgctgcttgg aggtgacccc cagtttgagg tagctgcggg cgaggtgtgt
      661 ctcgacggtg cgcacgccga tgtgcagccg ggtggcgatc tgcggtgcgg tgtagccacc
      721 ggaggccagc tcggcgacct gccgctcccg gggggtgagc gagcccgggc cgggcgtctg
      781 ggcggtgcac cgtccctgcg actcgagctg acggagcaac gcgcgggctt ggtcgcggcg
      841 gacaacggct ccgcaggcgt cgaatgccgc ggtggccgcg cgcaacgcct cctgcgctgc
      901 gcctcggtcc gaccgccgaa ctgcgttggc gtaggcgacg cgagcgcgcg ccgcgagcag
      961 cgcgtacccg ctggagtcga atccgtccat agcacccaac gctgctcgtg ccgccagatc
     1021 gtggcggccg cggcagatca gggcccacgc cgtggcgaac tggtgcagcg cctggtggat
     1081 cggtgcgccg gtgcggcgag cgttgtcttc agccgatgtc gccgccctcg ccgcacctcc
     1141 gggatcgccg atgaccaccg cgatctcggc cagatccgcg agaacgaagg cccgcaatgc
     1201 ccagacgttc atcaccgagt acccgtccac cgcccgttca aggacgctgg ccgcggccgc
     1261 caaccggccc tcggcccggg ccaccgttgc ctcggcccac cagtaccacg gctccagaat
     1321 gcccaacggc ctaccgttgg ctcgagtcat ggcgtcgagg tgccggcggg cctcaacgag
     1381 atggccgcgc tctgccgccg ccatcgccgc ccgggccgcc agccggaccg gtagccggaa
     1441 tcgatccgcc ggatggtggc cttgcgcctg ccgcgcatgt gcggcgacca tcgccagatc
     1501 tccagcg
//
LOCUS       DATADN010001215         4216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_894958, whole genome shotgun sequence.
ACCESSION   DATADN010001215 DATADN010000000
VERSION     DATADN010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894958"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VHH53_15550"
     CDS             complement(<1..738)
                     /locus_tag="VHH53_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX2291562.1"
                     /translation="MSAADHAGPFTERTPRVAAARALLRRRDRERAGRFLVEGAQAVR
                     EAVRYGSMQELFVTERAAARHPDLLAAAGSRVSLITERAAAGLSETATPQGVVAVCAA
                     LGRAAANALRGTRLVAVLVNVSDPGNAGTVIRVADAAGAGAVLFAGDTVDPHNGKCVR
                     ASTGSLFHLPLGVEPDPQRAVAACRQAGLQVLAADARRGCDLDDVIDAGTLAAPTAWL
                     FGSEAHGLDCALLAAADGAVAVPLHGPA"
     gene            complement(735..1139)
                     /gene="rplT"
                     /locus_tag="VHH53_15555"
     CDS             complement(735..1139)
                     /gene="rplT"
                     /locus_tag="VHH53_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX2291563.1"
                     /translation="MARVKRAVNAQKKRRSTLEAASGYRGQRSRLYRKAKEQILHSMQ
                     YAYRDRRARKGDFRKLWITRINAAARQNGMTYNRLVQGLRIAEIEVDRKNLAELAVSD
                     PTAFTALVEIAKANLPDSRGASAPVSREGDAA"
     gene            complement(1217..1411)
                     /gene="rpmI"
                     /locus_tag="VHH53_15560"
     CDS             complement(1217..1411)
                     /gene="rpmI"
                     /locus_tag="VHH53_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX2291564.1"
                     /translation="MPKNKTHRGTAKRVKLSATGKLLRQRAGKRHLLEKKSSRITRRL
                     DGVVEVAPQDTKRIRRLLGL"
     gene            complement(1549..2109)
                     /gene="infC"
                     /locus_tag="VHH53_15565"
     CDS             complement(1549..2109)
                     /gene="infC"
                     /locus_tag="VHH53_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX2291565.1"
                     /translation="MTVETRINERIRVPEVRLVGPNGEQVGIVRIEDALRLAQEADLD
                     LVEVAAQARPPVCKLMDFGKFKYETAQKARESRRNQQMTVIKEQKLRPKIDAHDYETK
                     KRHVVRFLDAGHKVKVTIMFRGREQSRPELGFRLLQRLSDDVAELGFVETSAKQDGRN
                     MTMVLAPHRNKPRGVKAERDTAQPAE"
     gene            complement(2446..4216)
                     /gene="uvrA"
                     /locus_tag="VHH53_15570"
                     /pseudo
     CDS             complement(2446..4216)
                     /gene="uvrA"
                     /locus_tag="VHH53_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467399.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
BASE COUNT          661 a         1421 c         1405 g          729 t
ORIGIN      
        1 cgcgggccca tgcagcggca cagcgacggc accgtcggct gccgcgagta gtgcacagtc
       61 cagaccgtgt gcctcgctgc cgaacagcca ggcggtcggt gccgccagag ttccggcgtc
      121 aatcacgtcg tcgagatcac agcctctacg ggcatcggcc gccaggacct gtaacccggc
      181 ctgccggcac gctgccacag cccgctgcgg atcgggctcg acgcccagcg ggaggtggaa
      241 caggctacca gtggaggccc gcacgcattt cccgttgtgc gggtcgacag tgtccccggc
      301 gaagagcacc gcgccggccc cggcagcgtc ggcgacccgg atgaccgtgc cggcatttcc
      361 tgggtcggac acattgacca gcaccgcgac cagccgggtg ccgcgcagcg cgttggccgc
      421 agcccgtccg agcgcggcac acacggccac aacgccttgt ggagtggccg tctcggatag
      481 ccccgcagcg gcccgctcgg tgatcagaga cactcgggaa cctgcggcgg cgagcaggtc
      541 aggatgccgg gcagcggccc gctcggtgac gaacaactcc tgcatcgatc cgtacctgac
      601 cgcctcgcgc acggcctgag ccccttcgac gaggaaccgc ccagcgcgct cccggtcccg
      661 ccgccgaagc aaggctcggg cagcagcgac ccggggagtt cgttcggtga agggaccagc
      721 gtggtccgct gcgctcaggc ggcgtcgccc tccctggaca cgggtgcgct cgcccctcgg
      781 ctgtcgggca ggttggcttt ggcgatctcg accagcgcgg tgaacgcggt cggatcggag
      841 acagcgagct cggcaaggtt cttgcgatct acctcaatct cagcaatgcg caggccctgg
      901 acgagccggt tgtaggtcat cccgttctgc cgtgcggcag cgttgatgcg ggtgatccaa
      961 agcttgcgga aatcaccttt gcgcgcccgc cggtcgcggt aggcgtactg catcgagtgg
     1021 aggatctgct ccttggcttt gcgatagagc cgggagcgct ggccacgata accactggcg
     1081 gcctccaggg tgctacggcg cttcttttgg gcgttgaccg ctcgcttcac gcgtgccact
     1141 ggattcgtcc tgtcggtctt ggggcggcgt agccacccag ggtggagtca cagtcagggg
     1201 tcggagaagc tggcggtcag agaccgagca gccggcggat ccgcttggtg tcctgcggag
     1261 cgacttcaac tactccgtcc agccggcgtg tgatccggga ggacttcttc tccagcaggt
     1321 ggcgcttgcc agcccgctgc cgcagcaact tgccggtagc ggaaagcttg acgcgcttag
     1381 cggtaccccg atgagtcttg ttcttcggca tgtccgtcct tgtcctgcca cccgctgggg
     1441 cggcggtcat ctgctcgtcg gcgcgtgacc agcagcgcga cgaccggccc gcacaccgac
     1501 gggcttgctt tggcggccca ggtgagccgc cggtccctgc accggctatt actccgccgg
     1561 ctgagcggta tcccgttcgg ctttgactcc ccgtggctta ttgcgatgcg gagctagcac
     1621 catcgtcatg ttccggccgt cctgcttggc tgaagtctcc acgaagccga gctcagcgac
     1681 gtcgtcggag agccgctgca gcaaccggaa ccccagctcc ggccgggact gctcgcggcc
     1741 ccggaacatg atggtgacct ttactttgtg ccccgcgtcg aggaaccgca cgacatggcg
     1801 cttcttcgtc tcgtagtcgt gggcgtcgat cttggggcgg agcttttgct ccttgatgac
     1861 cgtcatctgc tggttgcggc gggactcacg ggccttctgc gcggtctcgt acttgaactt
     1921 gccgaagtcc atgagcttgc acaccggtgg gcgggcctgc gccgcgacct cgaccaggtc
     1981 gagatctgcc tcctgagcta gtcgcagcgc gtcctcgatg cggacgatgc cgacctgctc
     2041 gccatttggt ccaaccaacc taacttccgg aacgcggatg cgttcgttga tgcgtgtctc
     2101 gacggtgatg tggcctcctc agtcgtcatg cccccggcgc gaccgagcac gcggggcctc
     2161 cttgctgacc cccggtgccc accaagacac ctcggacgca acaaggtccc gcactccgtc
     2221 tggcgtgcgg gacctgcaga tccgaccgat cgacgccgca tgtgtttcac caacacacga
     2281 cgtaacgccg aaatccacct gaggattccg ggaccggacc cgaccgcctc gcaacggttc
     2341 gggtgggagc aggctccact tgccacttcc gccggagcga aagctggtcg caccgacaga
     2401 ctagcagttc cagcatcaca ccggtgcgcg acgtgcgggt tggtgctatg agcctgcagc
     2461 cgcggtggcc cggcgacgcc gccgcggcgc cggctgctgc gcgtcgacga cggcgcgcag
     2521 gaactggccg gtgtaactgg tgtcgatagc ggcgacttgc tcgggcgaac cctcagcgat
     2581 cacggtgcca ccggctgccc cgccctcggg gcccatgtcg atcacccagt ccgacgactt
     2641 gatcacgtcg aggttgtgct cgatgacgat cacggtgttg cccttgtcgg ccaacccgtt
     2701 gatgacaccg agcagcttgc ggatgtcctc gaaatgcaag ccggtggtcg gctcgtcgag
     2761 cacgtagacg gtgcgcccgg tggaacgttt ctgcagttcg ctggcgagtt tgacgcgctg
     2821 ggcttcaccg ccggacaacg tcggcgccgg ctggccgagc cggacatacc ccagcccgac
     2881 gtccaccaat gttttgaggt ggcggtggat cgccgagatc ggctcgaaga acgccgccgc
     2941 ctcctcgatc ggcatgtcga gcacctcagc gatggtcttg cccttgtagt gcacctcgag
     3001 agtttcccgg ttgtaccggg cacctccaca gacctcgcac ggcacgtaga catcgggcag
     3061 gaagttcatc tcgatcttga tggtgccgtc gccggcacag gcctcgcagc gaccgcactt
     3121 gacgttgaag gagaaccgcc ctggctggta accgcggacc ttcgcctcgg tggtggcggc
     3181 gaagagcttg cggatccggt cgaacacgcc ggtgtacgtc gcggggttgg accgcggggt
     3241 acgcccgatc ggtgactggt cgaccttgac cagcttgtcg acctcgtcca gaccggtgac
     3301 ccgggtgtgt cgtccgggca cctgacgggc accgttgagc cggttcgcca gcacggtcgc
     3361 gaggatgtcg ttgaccagcg tcgacttgcc cgagccggag accccggtga ccgagatcag
     3421 gcagccgagc gggaaggaca cgtcgatgcc gcgcaggttg tgctcgcgtg ccccgatcac
     3481 ggtcagccgt cgcgaccggt ccaccggacg ccggacggtc gggaccggga tgctgcgccg
     3541 gcccgacagg taggcgccgg tgatcgaggt gtcatgctgc tccaactcat ggaacggacc
     3601 gctgtgcacc acctgaccac cgtgctcccc cgcgcccgga ccgatgtcca ccacccagtc
     3661 ggaagtgcgg atggtttcct cgtcgtgctc gaccacgatc agcgtgttgc ctagatcgcg
     3721 cagcctggtg agggtggcca gcagccggtg gttgtcccgc tggtggaggc caatcgacgg
     3781 ctcgtcaagc acatagagca ccccgaccag cccggagccg atctgggtgg ccagccggat
     3841 ccgctgcgcc tcaccaccgg acagcgtggc cgaggcacgg tccagggata ggtagtccag
     3901 cccgacatcg agcaggaaac gcagccgggc ctggatctcc ttgagcaccg caccggcgat
     3961 catcgcctgc cggtggtcca gcagcaggct ctcgaggaac gccgcagcgt cgcggaccga
     4021 cagcgcggac acatcggcga tggatttctt gcagtccgtg gtgtgagtaa gggtgaccgc
     4081 caggatctcg ggcttgagcc gggtgccgcg gcaggccggg cagggcacct cccgcatgta
     4141 gccgtcgtac ttctagcgca tgtactccga ctcggtctgg ctcatccggc gttcgaggaa
     4201 tgggatgacg ccttcg
//
LOCUS       DATADN010001216         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_293876, whole genome shotgun sequence.
ACCESSION   DATADN010001216 DATADN010000000
VERSION     DATADN010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293876"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..395
                     /locus_tag="VHH53_15575"
     CDS             102..395
                     /locus_tag="VHH53_15575"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX2291566.1"
                     /translation="MPTHTTSEAAPKAQEGSTTDLAALRTEIDDLDAEILRLVQRRTA
                     VSQAIGVARRAAGGPRVVHGREMAVLDRYRELGPEGHELAMLLLRLGRGRLGR"
     gene            466..771
                     /locus_tag="VHH53_15580"
     CDS             466..771
                     /locus_tag="VHH53_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291567.1"
                     /translation="MVHLIAVLAALAGLLTALGHGGYLAMLGSAANRRAAGDPVNQYV
                     RSRVPVAVATLGVALFALLLTTGGSFADVLAIVAGAGSGVVAYQALQGTRARFHSGG"
     gene            875..>1810
                     /locus_tag="VHH53_15585"
     CDS             875..>1810
                     /locus_tag="VHH53_15585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2291568.1"
                     /translation="MNADARLVADRYRLDQPIGAGAMGVVWRAHDMRLDRTVALKELL
                     MQPGLAEAEAETARARAMREGRIAARLQHPHAISVYDVALDGGSTGTGDRVVPWLVME
                     YLPSRSLATVLAEQGTLSPHEAARVGRQVAAALAAAHKAGIVHRDVKPGNILLGRNGT
                     VKITDFGISRASWDATVTRTGVLAGTPAYFAPEVARGELPGPAGDVFSLGSTLYAAVE
                     GEPPFGLDENTLALLRTVAEGRVGAPQQAGPLSAVLMQLLADDPLRRPSMSDAAAALG
                     AVESLAATASNTPGAIPPRRPPTTPGTAAPTALNLT"
BASE COUNT          280 a          628 c          630 g          272 t
ORIGIN      
        1 ttcaaaccat cacgacgccc cggagtccac ggacccgggg cgtttcgctg ccgggaaccc
       61 agggacacgg actccgggac agaatctagg aggccaccga gatgcccact cacacaacca
      121 gcgaggcggc gcctaaagcc caggaaggct ccacgaccga cctcgccgcg ctgcgtactg
      181 agattgacga cctcgacgcc gagatcctgc ggttggtgca gcgccgcacc gcggtgtcgc
      241 aggcgatcgg tgtcgcccgg cgagccgccg gtggcccgcg ggtcgtgcac ggccgcgaga
      301 tggctgtcct ggaccggtac cgggagttgg gccccgaggg ccacgagctg gcgatgctgc
      361 tgctgcgtct tgggcgcggc cggctcggtc gctgatcctc gcctatcccc gtttgtacgc
      421 ctgccgtcca ccgcctaccg gagccagagg gggcacactg gttccatggt gcatctgatt
      481 gctgtcctcg ccgcactcgc tggattgctg accgcgctgg gccacggcgg atacctcgcg
      541 atgctggggt cggcggcgaa ccggcgggcc gccggggacc cggtcaacca gtatgtccgc
      601 agtcgtgttc cggtggcggt agcgacgctg ggcgtggcgc tgttcgccct gttgctcacc
      661 accggcggct cgtttgccga tgtcctggcc atcgtggccg gagccggtag cggcgtggtg
      721 gcataccaag ctctgcaggg aacccgggcg cgctttcaca gtggcggctg atatctcgca
      781 accgcccagc ccactgaagg gaccggacac cgacgcctag ccgcctcgga ccgtcaagcc
      841 cctaccctga gcacgtgagc gaggagcgca gcaggtgaac gccgatgctc ggctcgtcgc
      901 cgatcgctac cgcctcgacc agccgatcgg cgccggcgcg atgggcgtgg tctggcgagc
      961 ccacgatatg cgactggacc gcacggtggc tctcaaggag ctgctgatgc agcctgggct
     1021 ggccgaggcg gaggcagaga cggccagagc cagggcaatg cgcgagggcc gcatcgccgc
     1081 tcggctccag catccgcatg cgatcagcgt gtacgacgta gccctcgacg ggggtagcac
     1141 cggtaccggt gatcgggtgg tgccgtggct ggtcatggag tacctgccgt cacgcagcct
     1201 ggccacggtt ctcgccgagc agggcacgtt gtcgccgcac gaggcggcaa gggtcggcag
     1261 gcaggtggct gccgcgctcg ctgccgccca caaagccggg atcgtgcacc gagacgtgaa
     1321 gccgggcaac atcctgctcg gtcgcaacgg gaccgtcaag atcaccgact tcggaatatc
     1381 tcgggcgagc tgggacgcca ctgtgacccg caccggcgta ctggccggca cgcccgctta
     1441 cttcgccccg gaggtggcgc gcggtgagtt gcccggcccc gctggtgacg tgttctcact
     1501 gggctccacc ctgtacgccg cggtggaagg agaaccaccg ttcgggctgg acgagaacac
     1561 cttggcgctg ctgcggaccg tcgctgaagg tcgggtgggg gccccacagc aggccggacc
     1621 gctgtccgca gtcctgatgc agctgctggc cgacgatccg ctgcggcgtc cctcgatgtc
     1681 cgatgcagct gccgcgctcg gtgcagtaga aagcctggcc gccacggcat cgaacacacc
     1741 cggggcgatc cctccgcggc gaccaccgac aacacccggg accgccgcgc ccaccgcgtt
     1801 gaaccttact
//
LOCUS       DATADN010001217         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_137133, whole genome shotgun sequence.
ACCESSION   DATADN010001217 DATADN010000000
VERSION     DATADN010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137133"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VHH53_15590"
     CDS             complement(<1..165)
                     /locus_tag="VHH53_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2291569.1"
                     /translation="MPGRVDPLVVRSAAGVRLQLADGTELVDGMSSWWAAIHGYAHPV
                     LDAAVQAQLGR"
     gene            565..1266
                     /locus_tag="VHH53_15595"
     CDS             565..1266
                     /locus_tag="VHH53_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2291570.1"
                     /translation="MPRNLAASTVAACDEYPDIPEQPGVAGRRAPNAIRVLLVIDLSL
                     LRGALAALLSSEDDIEVVAGMALGDRVIPTAIRYRPDILVVDVDQSGPDVLATTQALH
                     KRLPDCRVIVLASAQRPGLVRRALDVPVAGAVDKDAQPRRLLATIRQVAKGKRTIDPT
                     LAVAAIGVAGSPLTPRELTVLDLASGGASPTEIAQRLFLSRGTVCNYLSRVMTKLDAR
                     TRIDAIRIAHEAGWI"
     gene            complement(1245..3041)
                     /locus_tag="VHH53_15600"
     CDS             complement(1245..3041)
                     /locus_tag="VHH53_15600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2291571.1"
                     /translation="MAPAVDTVPRVLRTTLRRHPGALARLAAWSVPQMLPLLLSGYGV
                     AHALDDGFLADRPGIGLMWLGVLAVAVLAGALATRHVYRILAEIVEPFRDDLVERVVG
                     GALQRSTVLGGRSDSGAVARLTHQVEVVRDTFAGLLLLTRSFMVSAVAAVIGVAALTG
                     IVGLLVLPPLLAGLGLFVASLRTMMARQRAHVLADEGVAESASTLAHSLRDVVACGAE
                     ERACATVQQQVDAHAEAERTLARMAAIRTLSVAVGGWLPVVSLLVGAPWLLATGATLG
                     TIAGGLIYLTQGLLPALKALVDGLGIGGLKLVATLDRLVEAGERPSSARALQRHAPVG
                     HRLVLNRVTFSYGSHAEPVVDKLDLVIPEGQHLAIVGPSGAGKSTLAGLIAATHVPQR
                     GEVRLGGLPVVDLDPQALARHRVVIPQEAYVFAGTVEENLRYLHPTATRAELDAAVDA
                     LGLCSVVTRLGGYDAAVNSGMVSAGERQLLALVRAHLTAAPVLILDEATCHLDPVAEQ
                     RVERVLAARGGTLIVVAHRISSAMRAHRILLMDSTPGGATTVVSGSHRELLRIAPLYR
                     DLVHHWDDGPELPGITRVTGPSTPNCQIQPAS"
     gene            complement(3062..>3476)
                     /locus_tag="VHH53_15605"
     CDS             complement(3062..>3476)
                     /locus_tag="VHH53_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2291572.1"
                     /translation="GYRTELAAAPLSGGEVQRLGLARAFVQPYRLLILDDATSSLDTV
                     TERQVEAAMTTGERGKTRLIIAHRVATAARTDLVAWLDRGKLRSLGRHTDLWRDPDYR
                     ALFGTEATPAETPGRTETLGRRETQELPAVSAAVR"
BASE COUNT          596 a         1226 c         1143 g          511 t
ORIGIN      
        1 ccggccgagc tgcgcctgca ccgccgcgtc gagcaccgga tgggcgtaac cgtggatggc
       61 cgcccaccac gacgacatcc cgtccaccaa ctcggtgccg tcggccagct gcaatcgaac
      121 cccggcagcc gagcggacca ccaggggatc gacccgaccc ggcatcggcc cgtagggatg
      181 ccagacgtgc gcacgatccg cggcgagcag gtcctgtacc gagttcacag cccgcaagcc
      241 tacggtcgcc cccggatcag tacgccaatt atacacagag taacaacggt aacggtatgt
      301 acacggtgta acactccgcg atgaagcggc gatgacaagc tgtacaggat ccatggggat
      361 cctcatgtag tggatcctcc gggatcacta gtagggggca gcagggcgcg gtcaaccgtc
      421 tgggatggat gcggactccg tgtccttccc tggcactggg ggagaggaca gcccaatggc
      481 agcacgttgg cgaccatgcg cattccgccg caccaccgac ctcgttcgta tcccatgatc
      541 tcacgacacc ggaccagctc accgatgccg cgcaacctcg cggcgagcac cgtggccgcc
      601 tgcgacgaat accccgacat ccccgaacag ccgggggtgg ccgggcgtcg cgctccgaat
      661 gcgatccggg tactgctcgt catcgacctg agcctgcttc gcggagcgct cgccgcgctg
      721 ttgtccagcg aggacgacat cgaggtggtc gccgggatgg cgttgggcga ccgggtcatt
      781 cccaccgcga tccgctaccg gccggacatc ctcgtggtgg acgtcgacca gagtggaccc
      841 gacgtactgg cgaccaccca ggcgctgcac aagcggctac ccgactgccg ggtaatcgta
      901 ctggccagcg cgcagcgacc gggcctggtc cgtcgagcat tggatgtacc ggtggccggc
      961 gcggtggaca aggacgcgca gcctcggcga ctgctggcga ctatccgcca ggttgccaag
     1021 gggaagcgga ccatcgaccc caccctggcg gtggccgcga tcggtgtggc aggtagccca
     1081 ctcacgcccc gggaactcac ggttctcgac ctcgcctcag gcggggcgtc gccgaccgag
     1141 atcgcccaac ggctgttcct cagtcgcggc acggtatgca attacctctc gcgcgtcatg
     1201 accaagctag acgctcgcac ccggatcgat gccattcgca tcgctcatga ggcaggttgg
     1261 atctgacagt tgggtgttga cgggccggta actcgggtga tcccggggag ttccggcccg
     1321 tcatcccagt ggtggaccag gtcgcggtac agcggtgcaa ttctgagcag ctcgcggtgg
     1381 ctacctgaca ccaccgtcgt ggcaccgccg ggggtgctgt ccatcagcag gatccgatgg
     1441 gcccgcatcg cggagctgat ccggtgcgca accacgatca aggtgccacc acgtgcggcg
     1501 aggactcgct cgactcgctg ctcggcgacc ggatccagat ggcaggtggc ctcgtcaagg
     1561 atcagcacgg gcgctgcggt caggtgcgct ctgacaaggg caagcagttg ccgctcaccg
     1621 gccgacacca tgccggagtt caccgcggca tcgtatccgc ccaggcgggt taccacggag
     1681 cacaagccca gcgcgtcgac ggcggcgtcg agctcagctc gggtcgctgt gggatgcagg
     1741 tagcgcagat tctcctcgac tgtgccggcg aagacgtaag cctcttgcgg gatcaccacc
     1801 cggtgacggg caagggcctg tgggtcgagg tcgacgaccg gcagcccacc caagcggacc
     1861 tcgccccgct gcgggacgtg cgtcgctgcg atcagcccgg ccagggtgga cttccctgcc
     1921 ccgctgggcc cgacgatggc caggtgctga ccttccggga tcaccagatc cagcttgtcc
     1981 actaccggtt cggcgtggct gccgtaggag aaggtgacgc ggttgagcac cagacggtgc
     2041 cccacgggcg cgtgccgttg cagggcccgg gccgacgacg ggcgctcgcc cgcctccacg
     2101 agccgatcta gggtggccac cagcttcaag ccaccgatgc cgagcccgtc aaccagcgcc
     2161 ttcagcgccg gcaacaggcc ttgggtcagg tagatgagac cgccggcgat cgtgccgagt
     2221 gtcgcgccgg tcgccaacag ccagggcgct cccacgagca ggctcacgac cggtagccag
     2281 ccaccgaccg ctaccgacaa tgtgcggatc gccgccatgc gagccagcgt tctttccgcc
     2341 tcagcatgcg cgtccacctg ttgctgcacc gttgcgcaag cccgctcttc ggcgccgcac
     2401 gcaaccacgt cccgcaggct gtgggcaagg gtgctggctg attcggcgac cccttcgtcg
     2461 gcgagcacgt gggcccgctg ccgggccatc atggtccgca aggacgccac gaaaagacct
     2521 agaccggcca gcaacggcgg cagcaccagc agcccgacga tcccggtcag tgcagccacc
     2581 ccgatcaccg cggcgaccgc gctgaccatg aaactcctgg tgagcaggag caagccggcg
     2641 aaggtgtccc gtaccacctc aacctggtgg gtcagccggg cgaccgcgcc cgaatcggac
     2701 cggccgccga gcactgtgga ccgctgcagg gcgcctccca ccacgcgctc caccaggtca
     2761 tcgcggaacg gctcgacgat ctcggcgagg atccggtaga cgtgccgggt ggccaatgcc
     2821 cctgccagca ccgctaccgc cagcacgcca agccacatca acccgatccc gggacggtcg
     2881 gccaggaatc catcgtccaa cgcgtgggcc accccgtagc cggacagcag caacggcagc
     2941 atctgcggca ccgaccacgc agccagccgg gcaagcgcgc caggatggcg gcgcagcgtt
     3001 gtgcggagca ctcggggcac ggtgtccacg gctggggcca tgccggttgt cgtgtcgggg
     3061 gtcaccggac tgccgccgat actgcaggca gctcctgcgt ttcacgtctg cccagcgttt
     3121 cggttcggcc cggcgtttcc gcaggcgtcg cctcggtgcc gaagagagcc cggtagtccg
     3181 ggtcgcgcca cagatcggta tgccgaccca ggctgcgtag tttgccccga tcgagccagg
     3241 cgaccagatc cgtccgggcg gccgtagcca cccggtgtgc gatgatcaac cgagtctttc
     3301 ccctttcgcc ggtggtcatc gccgcctcga cctgccgctc ggtcaccgtg tccaggctcg
     3361 aggtggcgtc atcgagaatc agcaaccggt aaggctgcac aaacgcccgg gctaggccga
     3421 gccgctgcac ctcacctccg gacagggggg ccgcggccag ctcggtccgg tatccc
//
LOCUS       DATADN010001218         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1347156, whole genome shotgun sequence.
ACCESSION   DATADN010001218 DATADN010000000
VERSION     DATADN010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347156"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..>1856
                     /gene="fxsT"
                     /locus_tag="VHH53_15610"
     CDS             62..>1856
                     /gene="fxsT"
                     /locus_tag="VHH53_15610"
                     /inference="COORDINATES: protein motif:HMM:NF040586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxSxx-COOH system tetratricopeptide repeat
                     protein"
                     /protein_id="HEX2291573.1"
                     /translation="MRHPRQAVHRTIAVVDVEGFGDRRRTTPHQLTVRQGLYSALRQA
                     FDHTGISWTDCHHEDRGDGVLLLAPAEMPKGPFVEALPYALVAVLREYNNTHPAEDQI
                     RLRMVLHAGEIAYDDHGVTASSINLAFRLLDARPLKVALAESPGVLALVTSGWFFEEV
                     VRHSAVIDPATYRPIQVAVKETSTVGWVCLPDHPYPADATYLMAPPSDLTMPVRDLPH
                     ARSEDAPTGRVWNVPARSTVFTGREELLTALHTALEEKPSTAVVQALHGMGGIGKTAL
                     AIEYAHRHASEYDVAWWVPAEESALVGDRLAELAQALGLTTVTDPPTAAVARLLGTLR
                     ERERWLLIFDNAEEPAVLVRYLPGGGGHVLITSRNPGWQELASPVGVDVFDRGESITL
                     LRRRVPRLTDSEAGQVAETLGDLPLALTQAGAYLADTTIGVQSYLTLLAQRTTELLAQ
                     GAPATYPVSLAASVQIALDRLSVQSRAALQLLTLAAYLAPEPIPLTLFTTRPTLLPDP
                     LAGAAEDPLAFTALTQLLRQHGLARVEPTTLALHRLLAAILRTQPHLPTLAVQLLRAA
                     VPADEPSDNPPAWPAWRQLLPHVLVATDPVRN"
BASE COUNT          299 a          639 c          614 g          304 t
ORIGIN      
        1 caagcctgta gcgtgtgact ttgaccaccg agcaacaagc ggtgcgtcgc aaggggaaga
       61 gatgaggcat ccacgacagg ccgtgcaccg gaccattgct gtggtggatg tcgagggatt
      121 cggggatcgg cgacggacca cgccgcacca gctcacggtc cgtcagggcc tgtacagtgc
      181 cctgcggcag gcattcgacc atacgggcat ctcgtggacc gactgccatc acgaggaccg
      241 gggcgacggt gtgctccttc tagctcccgc ggaaatgccc aaagggccct tcgtggaagc
      301 cctgccgtac gcactcgtgg cggtgctacg cgagtacaac aacacgcacc cggcggagga
      361 ccagatccgg ctccggatgg tgctgcacgc tggagagatc gcctatgacg atcatggagt
      421 gacggcctcg tcgatcaacc tggcattccg gctcctggat gcacgcccgt tgaaagtggc
      481 gctcgcggag tccccgggcg tgttagcact ggtcacgtct ggatggtttt tcgaggaagt
      541 cgtgcggcac agtgcagtca tcgatcccgc cacctaccgg ccgattcagg tcgcggtgaa
      601 agagacttct accgtgggtt gggtctgcct gccggatcac ccctacccag ccgacgccac
      661 ctatcttatg gccccaccga gcgacctcac gatgccggtg cgggatctgc cgcatgcccg
      721 ctcggaggat gcgccgacgg gtcgggtgtg gaacgtgccc gcccgcagca cggtcttcac
      781 cggacgagaa gagctgctca ccgcgctgca taccgcgtta gaggaaaaac cctcgacggc
      841 ggtggtgcaa gccctgcatg ggatgggtgg gatcgggaag accgcgctag ccatcgagta
      901 cgcccaccgc catgcgagcg agtacgacgt ggcgtggtgg gttccggcgg aggaatcagc
      961 gctggtgggt gaccggctgg ccgagctagc ccaggcccta ggcctgacga ccgtcactga
     1021 ccccccaacg gcggcggtgg ctcggctgct ggggacgctg cgagagcggg agcggtggct
     1081 gctgatcttt gataatgccg aggaacccgc agtgctggtc cggtatctgc ccgggggcgg
     1141 tgggcacgtg ttgatcacct cccggaatcc cggctggcag gaactggcga gcccggtggg
     1201 ggtggacgta ttcgacagag gcgagtcgat cacgttgctg cgccgccgcg tgccccggct
     1261 cacggacagt gaggcggggc aggtcgcgga gaccctgggg gatctgccgt tggcgctgac
     1321 tcaggctggc gcgtacttgg ccgataccac tatcggtgtc cagagctacc tgaccctgct
     1381 cgcgcagcgg accacggagc tgctggccca aggtgccccc gccacgtacc cggtgtcgct
     1441 tgccgccagc gtccagattg ccctcgaccg gctgtccgtc cagtcccggg ctgcgctgca
     1501 gctgctcacc ctggcggcct acctggcgcc ggagccgatc ccgctgaccc tgttcaccac
     1561 ccgccccacc ctgctacccg accccttggc aggcgcggcc gaggatccgc tggcgttcac
     1621 cgcgctgacc cagctgctgc gccagcacgg gctggcccgc gtcgagccca ccaccctggc
     1681 gctgcatcgg ctgctcgccg cgatcctgcg cacccaaccc cacctgccca ccctggcggt
     1741 tcagctactg cgtgccgccg ttccggccga cgaaccatca gacaacccac cagcttggcc
     1801 ggcctggcgc cagctccttc cgcatgtctt ggtcgccacc gaccccgttc gcaacc
//
LOCUS       DATADN010001219         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1347297, whole genome shotgun sequence.
ACCESSION   DATADN010001219 DATADN010000000
VERSION     DATADN010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347297"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VHH53_15615"
     CDS             <1..704
                     /locus_tag="VHH53_15615"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX2291574.1"
                     /translation="PRVLIGLDPEWDWGQRELIELMADRCGVSADPRQTSGQDVIDPE
                     RTLAALDAFAERLACAAQRGAPVLLGTGHPHRLLGFYAALADALSAAGCAVLTPAQGR
                     CVDITTRFGLRTYNLGYVRGVALVRAPGAERPGCETGAHTHSPLPVRTVLAAAAEAGG
                     PLPELVIGDHGWVCGAGQLGFEAIGPADTDDPAPFVGQAEGSVSVVVPLDDGVRSDYY
                     LPLTRYVLNRACLSQ"
     gene            834..1046
                     /locus_tag="VHH53_15620"
     CDS             834..1046
                     /locus_tag="VHH53_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2291575.1"
                     /translation="MAAAGERPLNEVQFLTVAEVASVMRVSKMTVYRLVHSGHLPAIR
                     VGRSFRVPEQAVHEYLRESYVGVETA"
     gene            1203..1301
                     /locus_tag="VHH53_15625"
     CDS             1203..1301
                     /locus_tag="VHH53_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEX2291576.1"
                     /translation="MGSVIKKRRKRMAKKKHRKLLKRTRVQRRNKK"
     gene            1504..>2036
                     /locus_tag="VHH53_15630"
     CDS             1504..>2036
                     /locus_tag="VHH53_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2291577.1"
                     /translation="MGKVVLVTGVARPLGGRFVRRIQRDPEVDRVVAVDAVPPEHHLG
                     GADFIQADIRQPTIARVLAETGADTVVHLDVTGTPLGSGNRASLKETNVIGTMQLLGA
                     CQKSPSVRRLVVKSSTNVYGSAPRDPAVFTETTPPKSLPSGGFAKDTVEVEGYVRGFA
                     RRRPDVAVCVLRFANILG"
BASE COUNT          317 a          662 c          715 g          342 t
ORIGIN      
        1 accctcgcgt cctgatcggt ctcgatcctg aatgggactg gggacagcgg gaattgatcg
       61 agttgatggc ggaccggtgc ggggtttcgg ccgatcctcg tcagacttcc ggccaggatg
      121 tgattgatcc ggagcggacg ctggccgcac tggatgcctt cgcggagcgc ctcgcctgtg
      181 ccgcccagcg tggcgcaccg gtcctcctcg gcaccgggca cccccaccgg ctgctcggtt
      241 tctacgccgc cctggcagac gcgctgtcgg cggcgggatg tgccgtcctc accccggcgc
      301 agggtcgctg tgtcgacata acgacccggt tcggtctacg tacgtacaac ctcggctacg
      361 tacgaggagt cgcgctggtg cgggcacccg gcgcggaacg ccccggttgt gagaccggcg
      421 cacacacgca ttctcccctc ccggttcgta ccgtgctggc tgcggccgcg gaagccggcg
      481 ggccccttcc cgagctggtg atcggggacc atgggtgggt ctgcggggca ggtcagctgg
      541 ggttcgaggc catcgggccg gccgacacgg acgatccggc tccgttcgtc gggcaggcgg
      601 aggggtccgt ctccgtcgtc gttccacttg atgacggtgt gcggtctgat tactacctgc
      661 cgcttacccg ctacgtactc aatcgggcgt gtctgtcaca gtaggccgcc gatgggtgca
      721 cctcttcccc acttgcatca cccgccccta cattggggag tgagcacgca gcgacgaaga
      781 gtcaccggaa ggggaagccg gtggccgtcg agtgcggaag gttcaggtgt gtcatggctg
      841 cagctggcga gaggcctctg aacgaggttc agttccttac cgtggcggaa gtcgcctcgg
      901 tgatgcgagt gtcgaagatg accgtgtacc ggttggtgca cagcggtcat ctgcccgcga
      961 tccgtgtggg gcggtccttc cgcgtcccgg agcaagcggt tcacgagtac ctccgcgaga
     1021 gttacgtggg ggtggaaacc gcctgacggc tagggcgggg ggatcctcac agggcacttc
     1081 aggatctccc cggccctctc gattacgacc tcagcgctcg ggcgggtagg ctagcccctc
     1141 gtaggtcgtg tgggcccatg gcgcccaaac aaccgagtga tgagaagtga gcgagggtag
     1201 tcgtgggctc tgttatcaag aagcggcgca agcggatggc caagaagaag caccgcaagc
     1261 tgctcaagcg cacgcgcgtt cagcgtcgca acaagaagta agttcgcgac gcgacgccgc
     1321 gagagcgttg ctgtggcccc ccacccaccg gtggggggcc acatgtgttc cggctcgtac
     1381 gtccggctgc ttgtcgtcac ttcgcgcatc gggcggtcat cacagcgcaa cgccgacccg
     1441 ctaagttggc cccacggggg aacgcggcgg ggcaagaggt tttctggaag gaaggcgctg
     1501 atcttgggca aggtcgtgct cgtgaccgga gtagcccgcc cgctgggggg ccggttcgta
     1561 cggcggatcc agcgtgaccc cgaggtggac cgggtcgtcg ccgtggacgc ggtgccgccc
     1621 gagcaccacc tgggcggcgc ggacttcatc caggccgaca tccggcagcc caccatcgcg
     1681 cgcgtgctcg ccgagacggg cgccgacacg gtcgtccacc tggacgtgac gggcacaccg
     1741 ctgggcagcg gcaaccgggc ctccctgaag gagaccaacg tcatcggcac catgcagctg
     1801 ctcggtgcct gccagaagtc cccgagcgtg cggcggctgg tcgtgaagtc cagtacgaac
     1861 gtctacgggt ccgcaccccg cgacccggcc gtcttcaccg agacgacccc gcccaagtcc
     1921 ctgcccagcg gcggcttcgc caaggacacg gtcgaggtgg agggatacgt acgagggttc
     1981 gcgcgccgcc ggccggacgt cgccgtgtgc gtgctcaggt tcgccaacat cctggg
//
LOCUS       DATADN010001220         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_137490, whole genome shotgun sequence.
ACCESSION   DATADN010001220 DATADN010000000
VERSION     DATADN010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137490"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VHH53_15635"
     CDS             complement(<1..936)
                     /locus_tag="VHH53_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2291578.1"
                     /translation="MLRRTVLPSGLRVVTEELAGSHSAVVGMWVAVGSRDEPTAVAGA
                     AHYLEHLLFKGTARRCAATIAEEIDAVGGELNAFTAKEHTCYYAHVLDSDLELAVDLV
                     CDVLGDALLDPSDVDLERGVVLGEIAMRDDDPEDLLDDEFCAALFGLHPLARPVLGTE
                     ESVTGMTREALHRFYRRCYTPERMVLAVTGNVTHQAVLAAVQDSFSHHLTGVAQPVGP
                     RRGQAAVTGGRRLAVCNDHSEQAHLMVGVPALDRHDDRRTVLGVLNAALGGGMSSRLF
                     QQVRERRGLAYSVYSSVACYADTGSLSIYAGCSPQR"
     gene            complement(963..>1629)
                     /locus_tag="VHH53_15640"
     CDS             complement(963..>1629)
                     /locus_tag="VHH53_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX2291579.1"
                     /translation="VTGTADGVTALQMDIKVAGITTEIMRDALKQASDARMHILGKMT
                     EVISASREELSPFAPRITTIKIPVDKIGEVIGPKGKMINSITEETGADISIEDDGTIY
                     VGANDGLSAEAAIDRINAIANPQLPKVGERFLGTVVKTAAFGAFVSLVPGKDGLVHIS
                     KLGNGKRIGKVEDVVKVGDKLRVEVADIDNRGKISLVPVQDDDAADSGSGGETVIEEP
                     AST"
BASE COUNT          268 a          568 c          511 g          282 t
ORIGIN      
        1 ccgttgtggg gagcaaccag cgtagatcga caggcttccg gtgtcggcgt aacaggccac
       61 cgaggagtac accgagtagg ccaacccgcg ccgctcgcga acctgctgga atagccgtga
      121 actcataccg ccgcccagtg cggcattgag cacgcccagc accgtgcgcc ggtcgtcgtg
      181 ccggtccagg gctggcaccc ccaccatgag gtgggcctgt tcggaatggt cattgcacac
      241 agctagccgg cggccgccgg tgactgcggc ttggccgcgg cgcggaccta ccggttgcgc
      301 cacaccggtc aggtgatggg aaaagctgtc ctgtaccgcg gccaacaccg cctgatgggt
      361 cacattgccg gtcaccgcca gcaccatccg ctccggcgtg tagcaccgcc ggtagaagcg
      421 gtgcagcgcc tcgcgagtca tcccggtcac cgactcctcg gtgcccagca ccgggcgagc
      481 cagtggatgc aggccgaaca acgcggcaca gaactcgtcg tccagcagat cctccgggtc
      541 gtcgtcgcgc atcgcgatct ccccgagcac cacaccacgc tcgaggtcaa cgtcggaggg
      601 gtcgagcagc gcgtcaccga gcacgtcgca caccaggtcg acggccagct cgagatccga
      661 gtcgagcacg tgcgcgtagt agcaggtgtg ttccttggcg gtgaacgcgt tgagttcacc
      721 gcccaccgcg tcgatctcct cagcgatcgt cgccgcgcaa cgccgcgcag tgcctttgaa
      781 cagcaaatgc tccaggtagt gcgccgcacc agccaccgcg gtgggttcgt cgcgggaacc
      841 caccgccacc cacatgccga cgacggcgga atgcgaaccg gccagctcct cggtcaccac
      901 tcgcaatccg gagggcagca cggtgcgccg aagcatcgcg ccgtcgccgt cgtgccatga
      961 ggtcacgtgc tggccggctc ctcgatgacc gtctccccgc cagaaccact gtcggcggcg
     1021 tcgtcgtctt gtactggtac cagactgatc ttgcctcggt tgtcgatgtc ggccacctcg
     1081 acgcgcagct tgtcgccgac cttgaccaca tcctcgacct tgccgatgcg cttgccgttg
     1141 cccagcttgg agatgtgcac caagccgtcc tttccgggca ccagcgagac gaaggccccg
     1201 aaggcggctg tcttcaccac agtgccaagg aaccgctccc cgaccttggg cagctgcgga
     1261 ttggcgatcg cgttgatccg gtcgatggcg gcctcggcgg acaggccgtc gttggcgccg
     1321 acatagatag tgccatcgtc ctcgatggag atgtccgcgc cagtctcctc ggtgatcgaa
     1381 ttgatcatct tgcccttcgg gccgatcacc tcgccgatct tgtcgaccgg gatcttgatc
     1441 gtggtgatgc gcggagcgaa cgggctcagc tcctcgcggc tggcgctgat gacctcggtc
     1501 atcttgccca ggatgtgcat ccgggcgtcg gacgcctgct tcagggcatc gcgcatgatc
     1561 tcggtcgtga tgccggccac cttgatgtcc atctgcaggg ccgtcacgcc atcggcggtc
     1621 cctgtgacc
//
LOCUS       DATADN010001221         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_137787, whole genome shotgun sequence.
ACCESSION   DATADN010001221 DATADN010000000
VERSION     DATADN010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137787"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..>1513)
                     /locus_tag="VHH53_15645"
     CDS             complement(222..>1513)
                     /locus_tag="VHH53_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2291580.1"
                     /translation="SSTVADRPLAYQRLERQPHNANDWYVVAAARTPVGPLYGVSGWS
                     IALVVVALALLIFAAISFLAQQRLLVATSMTDALTGIPNRRQLKADLEQGLKQASRDR
                     PLLLMLFDLNGFKGYNDTFGHPAGDALLIRLAKALDAAMTEHGGTAYRLGGDEFCVLA
                     TVDPDNTTPIVAAAAQALSEHGGGFSITASYGSILLPQESQDPTEAMQLVDQRMYAQK
                     TSARRSPERQTRDVLLRALQERNPELAQRHAEVARLVVAVAERMGLPHDEHTQLRQAA
                     ELHDVGKLGIPEEILHKPAPLDADEWAFVRRHPLIGERIIGAAPALAPAARLVRSTHE
                     RFDGSGYPDGLAGDQIPLGARIIAVCDAFTAMTSSRPYAAQRTVPDAIAELRRGAGAQ
                     FDPAVVDLFSEMIVELVWPPERSTATAGNVDHVRDHA"
BASE COUNT          218 a          470 c          577 g          248 t
ORIGIN      
        1 tgtcacactc acgctcctgg tggcgtgccg ctgcgcttgg tggcctgctc cgacgcacac
       61 tgctcgtgct ggcgtaacct cctcatcggt aggttgcttg cctggctgca gcatggacgg
      121 agttgcgaag cgggcacgac ctaggtccgc ccagacgaaa ctcactgctc gacatgaccg
      181 cagaacttcg tgaggtcaag gccgctggag ttagcggcgg cttatgcgtg gtcgcgcaca
      241 tgatcgacgt tgccggcggt ggcagttgac cgctctggcg gccagaccag ctcgacgatc
      301 atctcggaga acaggtcaac gacggcgggg tcgaactggg cgccggcgcc ccgacgaagt
      361 tcggcgattg catcggggac ggtgcgctgc gccgcatagg ggcgggagga ggtcatggcg
      421 gtgaacgcgt cacagacggc gatgatccga gcccccagcg ggatctggtc gccggccagt
      481 ccgtcggggt agccgctgcc gtcgaagcgt tcgtgggtgg agcggaccag cctggcggca
      541 ggtgccaggg cgggcgcggc cccgatgatg cgctcgccaa tcagggggtg gcggcgcaca
      601 aacgcccact catcggcatc cagcggggcc ggcttgtgca ggatctcttc ggggatgccc
      661 agcttgccga cgtcgtgcag ctcggccgcc tgccgtagct gggtgtgctc atcgtgcggg
      721 agccccattc gctcggcgac ggccacgacg agcctcgcta cctctgcgtg gcgttgggcc
      781 agctcgggat tgcgttcctg caacgcccgc aggagcacat cgcgcgtctg gcgctcgggg
      841 gagcgccggg cactggtctt ctgggcgtac atgcgctggt cgaccagctg catggcctcg
      901 gtggggtcct ggctttcctg ggggagcagg atcgatccgt aggaggcggt gatggagaac
      961 cccccgccgt gctcgctgag cgcctgtgcg gccgcggcca cgatgggggt ggtgttgtcg
     1021 gggtcgacgg tggccaggac gcagaattcg tccccgccga ggcggtaggc ggtgccgccg
     1081 tgttcggtca tggcggcatc cagggccttg gcgaggcgga tgagcagggc gtcgccggcc
     1141 gggtggccga aggtgtcgtt gtagcccttg aacccgttga ggtcgaacag catcagcagc
     1201 aggggccggt cccggctggc ctgcttcagc ccctgctcca ggtcggcctt gagctggcgc
     1261 cggttgggga tgccggtcag ggcgtcggtc atcgaggtcg cgaccagcag ccgctgctgg
     1321 gccaggaagg agatggcggc gaagatcagc agggccaacg ccacgacgac gagggcgatc
     1381 gaccagccgc tgaccccgta caggggaccg accggggtgc gggcggcggc gaccacgtac
     1441 caatcgttgg cgttgtgggg ctggcgctcc aatcgctggt aggccagcgg acggtccgcg
     1501 accgtgctcg acc
//
LOCUS       DATADN010001222         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1347966, whole genome shotgun sequence.
ACCESSION   DATADN010001222 DATADN010000000
VERSION     DATADN010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347966"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VHH53_15650"
     CDS             complement(<1..1410)
                     /locus_tag="VHH53_15650"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2291581.1"
                     /translation="MSSTDRERAVVDAVPTELFINGRWRPAGGGKTFDVEDPSTGKAL
                     RAVADAAPEDGLAALDAAVAAQARWAQHPPRERGEILRRAYELMMQRQDDLALLMTLE
                     MGKPLAESQAEIAYAAEFFRWFAEEAVRIDGGFATAPNGSGRMLVMRQPIGPSLLITP
                     WNFPAAMGTRKIGPAVAAGCTMVLKPARLTPLSMLALAEILAECGLPDGVLNVVPTTS
                     SGRLMEPLIRDGRARKLSFTGSTEVGRMLLEQCAEQILRTSMELGGNAPFLVFADADL
                     DAAVGGAMIAKMRNIGEACTAANRFFVHTDIAEEFSRRLAEQMGSLRIGRGSEEGVQV
                     GPLIEAKARDKVQHLVDDAITRGARVLVGGEPVDGPGYFYRPTVLAGVPPDAEMARTE
                     IFGPVAPITPFTDEDEVLATANGTSFGLASYVYTEDLRRALRVCERLECGMVGLNQGL
                     VSNPAAPFGGVKHSGLGREG"
     gene            complement(1420..>2049)
                     /locus_tag="VHH53_15655"
     CDS             complement(1420..>2049)
                     /locus_tag="VHH53_15655"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEX2291582.1"
                     /translation="LVALIVAALVLPVPTALQMRDWAQSVGIAAPMLFLLGLTVVTIA
                     PIPRTVFTLAAGLLFGPVLGVVLSLVATTLAAALAFVVVRRVGRSLVEPHLDRGLLRA
                     IDARLRRRGWLAVASLRLIPAVPFSVLNYCSAVSSIRFRDYLVGTVGVIPGSVALVVL
                     GDALTGTTSPALLAISLGGAAIGVIGLVVQGRSGALEPPTVEPPVTVPK"
BASE COUNT          358 a          737 c          682 g          272 t
ORIGIN      
        1 gccctcgcga cccaggccgg agtgcttgac gccgccgaag ggtgccgccg ggttggacac
       61 caggccctgg ttgagcccga ccatgccgca ctcgagccgc tcacagaccc gcagagcacg
      121 gcgcagatcc tcggtgtaga cgtagctggc cagcccgaag gaggtgccgt tggcggtcgc
      181 cagcacctcg tcctcgtcag tgaacggggt gatcggcgcg accggtccga agatctccgt
      241 gcgggccatc tcggcatccg gcggcacgcc agcaagcacg gtggggcggt agaaataacc
      301 gggaccgtcc accggctcac cgccgacgag cacccgggcc ccccgggtga tcgcgtcgtc
      361 gacgaggtgc tgcaccttgt cccgcgcctt ggcctcgatc aacgggccga cctgcactcc
      421 ctcctcgctg ccacggccga tgcgcaacga gcccatctgc tcggccagcc gccgggagaa
      481 ctcctcggcg atgtcggtat gcacgaagaa gcggttggca gcggtgcagg cctcgccgat
      541 gtttcgcatc ttcgcgatca tcgccccccc gaccgcggca tcgaggtcgg cgtcggcgaa
      601 gaccaggaac ggcgcattgc caccgagctc catcgaggtt cgcaggatct gctcggcgca
      661 ctgctccagc agcatgcggc ccacctcggt ggagccggtg aaggacagct tgcgggcccg
      721 gccgtccctg atcagcggtt ccatcagccg gcctgagctg gtggtgggca cgacgttgag
      781 caccccgtcg ggcagcccgc attcggccag gatctcagcc agcgcgagca tcgacagcgg
      841 ggtcaagcgg gccggcttca gcaccatcgt gcaaccggcc gccaccgccg gaccgatctt
      901 gcgggtaccc atcgcggccg ggaagttcca cggagtgatc agcagtgacg gcccgatcgg
      961 ctggcgcatc acgagcattc gccccgagcc gttgggcgcg gtagcgaacc cgccgtcgat
     1021 gcgcaccgcc tcctcagcga accagcggaa gaactcggca gcgtaggcga tctcggcctg
     1081 cgactcagcc aacggcttgc ccatctccag ggtcatcagc aacgcgaggt cgtcctggcg
     1141 ctgcatcatc agctcgtagg cgcggcgcag gatctcgccc cgttcccggg gcgggtgctg
     1201 cgcccaccgg gcctgggcgg cgacggcggc gtccagtgcg gccagcccgt cttcgggcgc
     1261 agcgtcggcg actgcgcgca gcgccttgcc ggtcgagggg tcttcgacat cgaacgtctt
     1321 cccgccgcca gcgggtcgcc accgaccgtt gatgaacagc tcggtgggga cggcgtcgac
     1381 gacggcgcgc tctcggtcgg tggaactcat ggcatcacgc tacttgggta ccgtcaccgg
     1441 gggctccaca gtcgggggct ccagagcccc actgcgccct tgcacgacca ggccgatcac
     1501 cccgatggcg gcgcctccga gcgagatcgc gagcaacgcc ggtgaagtgg tgccggtcaa
     1561 ggcgtcgccg agcacgacga gcgcaaccga cccgggtatg acgcccacgg tgcccacgag
     1621 gtagtcgcga aaccggatcg acgacaccgc gctgcaatag ttgagcaccg aaaacggtac
     1681 cgccggaatc aatcgcagcg acgccacggc gagccaacca cggcggcgca gtcgcgcgtc
     1741 gatcgcccgt agcagcccac gatcgaggtg aggttccacc aggctgcgcc ctacccggcg
     1801 aaccacgaca aaggccagtg ctgcagccaa cgtcgtcgcc accaatgaca acaccactcc
     1861 cagcacgggt ccgaacagca accccgcggc aagggtgaac acggtgcggg gaatcggtgc
     1921 gatcgtcacc acggtcagcc cgagcaagaa cagcatcggt gcggcgatgc cgaccgactg
     1981 ggcccaatcc cgcatctgca acgcggtggg caccggcagc accagcgccg ccacgatcag
     2041 cgcgacgag
//
LOCUS       DATADN010001223         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_295183, whole genome shotgun sequence.
ACCESSION   DATADN010001223 DATADN010000000
VERSION     DATADN010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295183"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VHH53_15660"
     CDS             complement(<1..415)
                     /locus_tag="VHH53_15660"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX2291583.1"
                     /translation="MSAESTVTIGLLLPDVLGTYSDAGNATILARRLHWRGISAEILT
                     ITARDAPATTCAIYVLGGGEDAAQQFAAEWLRRHDVLHRALESSAITLAVCAGLQILG
                     RSLIDSSGHHYPGLGLLDLVTAPGSQRAVGEVLSTC"
     gene            complement(412..1740)
                     /locus_tag="VHH53_15665"
     CDS             complement(412..1740)
                     /locus_tag="VHH53_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEX2291584.1"
                     /translation="MVQTSGLEPAAVLHPRRRRLGWWTRAAVQAGLAAAALSRLLGLG
                     TGSIIGGRVTLALDHRALHRLAAGRRVVLVSGTNGKTTTNHLIAAAVRTVGPLAHNAG
                     GSNMSDGVVAALADAPDAGYAVIEVDELHLAAIADAVDPMVVVLLNLTRDQLDRATEV
                     GAIAAAIRRALARHPHTVVVANADDPMVVSAVGPASRAVWVAAGASWLGDVATCPRCG
                     HILTFTNSDVAIGSARGGRDEQGWDCACGLARPTPDWWIEDGKACTIETLVPLEIRLP
                     GQINMGNGVMALAGACAVGVPADQAAPALGQVEVVAGRYATVHRGAQALRMLLAKNPA
                     GWAGMLSILSAPAPLLVIINAREADGRDTSWLWDVPFERLSSRPVVASGERAADLGVR
                     LSYAGLEHQTVSDPLVALAALPEGAVDVVANYTAFRHLRQRLSQERQAVG"
     gene            complement(2066..>2335)
                     /locus_tag="VHH53_15670"
     CDS             complement(2066..>2335)
                     /locus_tag="VHH53_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291585.1"
                     /translation="RQSRCPRPSADHGAQDRSVATTAGRPKLRYATVGWLLVVLAVGT
                     VVLIRGATRYNPYLKNLRAVEPTPSVTCWSASVLRPIGSVPGLGQ"
BASE COUNT          411 a          826 c          715 g          383 t
ORIGIN      
        1 cacaagtgct cagcacctcg ccaacggcgc gctggctgcc tggggcggtg acgagatcca
       61 gcaggcctag tcccgggtaa tgatggccac tggaatcgat caacgaccgt cccagaattt
      121 gcagtccagc gcacacggcg agggtgatag cgctgctttc cagtgctcga tgtagcacat
      181 catgacgacg cagccattct gcggcgaatt gttgagcggc gtcctctccg ccacccaata
      241 cgtatatcgc gcacgtcgtc gccggggcgt cgcgagcagt gatagtaaga atttcggcgg
      301 aaatgccgcg ccagtgtagc cgtctagcca gtatcgtggc attgccggcg tccgagtatg
      361 tacccaggac atcgggcaat agcaggccga tcgtgaccgt cgattctgcg ctcacccgac
      421 tgcttgccgt tcctggctca gccgttggcg aaggtgacgg aaggcggtgt agttggccac
      481 cacgtccacg gccccctccg gtagtgccgc cagggcgacc aacgggtcgg agacggtctg
      541 gtgctccagc ccggcatagc tgagccggac accgaggtct gcggcacgtt ctccgctggc
      601 caccaccgga cgggagctga gccgttcaaa aggaacgtcc cacagccagg aggtgtccct
      661 cccgtcggcc tcccgggcgt tgatgatcac cagcagtggc gctggcgcac tcagtatgga
      721 cagcattccg gcccatccag ccgggttctt ggccagcagc atgcgcaggg cctgcgctcc
      781 gcggtgcacg gtggcgtagc ggccggccac cacctccacc tggcccagtg ctggggcggc
      841 ctggtcggcc gggacaccga cggcgcaggc gccagccagc gccatgacgc cgttgcccat
      901 attgatctga ccgggcaacc ggatttccag tggtaccaag gtctcgatgg tgcatgcctt
      961 gccgtcctcg atccaccagt ccggggtggg tcgggccaac ccgcacgcac aatcccaccc
     1021 ctgctcgtca cggccaccgc gggcggaccc gatagcgaca tcggagttcg tgaacgtgag
     1081 gatgtggcca cagcgcgggc aggttgccac atcacccagc cagctggctc ccgccgctac
     1141 ccacacggcg cgcgacgccg gcccgaccgc cgacacgacc atcggatcat cagcgttagc
     1201 cacgaccacg gtgtgcgggt gccgggccaa cgcacgccgg atggcggccg cgatcgcgcc
     1261 tacttcagtc gctcggtcca gttggtcacg agtcaggttc agcagaacga ccaccatcgg
     1321 gtcgactgcg tcggcgatgg cagccaggtg cagctcgtca acctcgatca ccgcgtagcc
     1381 ggcgtccgga gcatcggcca gcgccgccac taccccatcg gacatattcg agccgccggc
     1441 gttgtgcgcc agcggcccca cagtgcgcac cgccgccgcg atcagatgat tagtcgtggt
     1501 cttgccgttg gttcccgaca ccaacaccac ccgccgccca gccgccaagc ggtgcagtgc
     1561 acggtggtcc agggccaacg tcacccgacc accgatgatc gatccggtcc ccagtcccag
     1621 cagccgggac aacgctgccg ccgccaaccc cgcctgcacg gcagcgcgcg tccaccaccc
     1681 caaccgacgt ctccggggat gcagtaccgc cgccggctcc agcccggatg tctgcaccat
     1741 cgtcgccccc aagcctctcc gcccccgcct gcggtcagtg ggcggagcat cacccaccag
     1801 catgggcctg gcgaaggtcc caccgtggcc tgatcgcccc cgtcagccat gagagggctg
     1861 acctcagtat tccccaccag ggcagttctg aatcaacatt ctgtctggta tccactcaac
     1921 aggccgccgc cgtcgctcgc tcaacagtct gttgagttcg gggcctccta gatcaggtca
     1981 actcaatgat cactgccgat tttgcgccgc tgggatggga tcgaggttgc cctgcgtgtc
     2041 tcgatctttc acatatccgg ggaagtcact gccccagccc tggtaccgat ccgatcggcc
     2101 ggagcaccga ggctgaccag caggtcacgg acggcgtcgg ctcgaccgca cgtaggttct
     2161 tcaggtacgg gttatacctg gtcgcaccgc gaatcaggac cactgtgcca acagcaagca
     2221 ctacgagcag ccagccgacc gtggcgtatc tcagcttcgg ccgaccagcg gtggtcgcca
     2281 ccgatcgatc ttgtgctcca tgatcagcgg atggccgtgg gcaccgagat tggcg
//
LOCUS       DATADN010001224         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_138479, whole genome shotgun sequence.
ACCESSION   DATADN010001224 DATADN010000000
VERSION     DATADN010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138479"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VHH53_15675"
     CDS             complement(<1..818)
                     /locus_tag="VHH53_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2291586.1"
                     /translation="MLDLETEAVAASWTVVDDRLTRWQAGFEEVFGLVAGRFAQVQSR
                     RRARWYLLGLLSGAERKNSWTIAKQAGELSPDGMQRLLNFYRWDADAVRDDLRAYVLS
                     NLGDPAGVVVADETGFLKKGTTSAGVQRQYSGTAGRIENCQLGVFITYVSAAGRALID
                     RELYLPKTWTDDRSRCAEAGIGEEVDFATKPELARRMLARLLTHHGRAAMPWFTADEV
                     YGDNPGLRGWLDEQQLNYFMAVSCDARFATPTGPRRADELAASAPKRGWQRLSAG"
     gene            complement(1056..1487)
                     /locus_tag="VHH53_15680"
     CDS             complement(1056..1487)
                     /locus_tag="VHH53_15680"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HEX2291587.1"
                     /translation="MDFLGFHNRLVRGRTPRSAHLVFLARWPARKAAQHARDRIRSIT
                     ARSRLLIPVERIVEELNLFLRGWAGYFRYGNSALLLGQIRNYALTRLALWLSKRGNRR
                     RAWGWGMTQVLLSPNHLGLISLDGIVVPPRPFRAWKVECRR"
     gene            complement(1576..2412)
                     /locus_tag="VHH53_15685"
     CDS             complement(1576..2412)
                     /locus_tag="VHH53_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX2291588.1"
                     /translation="MSADQASSTAVGSRRDLVRALQHALYRSAKADPGRGFHALRDKV
                     YRRDVLWRAWVAVRRNAGAPGIDGTTLADVEEYGVDRLLDELAEDLRTGQWRPLPSRR
                     VLIPKPGTSERRPLSIPRVADRIVQAAVKIVLEPIFEADFLSCSFGFRPKRSAHDALQ
                     VLIDESWQGARWVVETDIANCFSAIPHEKLMQAVEERISDQGIMKLLRAMLRAGVMAD
                     GAVRREVSGTPQGGPISPLLCNVYLHRLDRAWDTREHGVLVRYCDDLVVMCQSRNKPT
                     PP"
BASE COUNT          514 a         1057 c          833 g          494 t
ORIGIN      
        1 ccggccgaga ggcgttgcca accgcgtttg ggtgcggacg cggcgagctc atcggcccgg
       61 cgcggcccgg tgggggtagc aaaccgggca tcgcaggaca cggccatgaa gtagttgagc
      121 tgctgctcgt caagccaacc ccgaagccct gggttgtcgc cgtagacctc gtccgcggtg
      181 aaccatggca tcgcggcccg gccgtggtgg gtcagcaacc gggccagcat gcgcctggcc
      241 agttcgggtt tggtggcgaa gtcgacctcc tcaccgattc cggcctcggc gcaccggctg
      301 cggtcgtcag tccaggtctt gggcaggtac agctcgcggt cgatcagcgc ccgccccgcc
      361 gcagacacgt aggtgatgaa cacgccgagc tggcagttct cgatccggcc agcggtgccc
      421 gaatactggc gctgcacacc ggcggacgtg gtgcccttct tcagaaaccc ggtctcatcc
      481 gcgaccacca ccccggccgg atcacccagg ttgctcagca cgtaagcacg caggtcatcg
      541 cgcaccgcgt cggcatccca gcggtaaaag ttcagcagcc gctgcatccc gtccgggctg
      601 agctcaccag cctgcttggc gatcgtccac gagttcttcc gctccgcgcc cgagagcagt
      661 cccagcaggt accaccgcgc ccgccgccgc gactgaactt gcgcgaaccg gcccgccacg
      721 agtccgaaca cttcctcgaa cccggcctgc caccgcgtca atcgatcatc gactaccgtc
      781 catgacgcgg ccaccgcctc agtttcaaga tccaacatac cttgaagttg atcacgcggt
      841 ggccgccttc acgatcacga cacgccgacc aggatcacac tctgggaggg tcggaggctg
      901 gcgcggtggc gttttaatca gctgtaggtc tgggcgcttc tatcgggggt ttccccgcgg
      961 tagctttcgc gtcgaccatg gctgacgctc cgtttccagc ctccgcccgt caaaccgtgc
     1021 atgcggttct cccgcacacg gcttaccgac gttcttcacc ggcggcattc gactttccag
     1081 gcccggaagg gtctgggcgg gacgacgatc ccatcgaggc tgatcagacc cagatgattc
     1141 ggagacagca gcacctgggt catgccccat ccccaggccc ggcggcggtt gccccgtttg
     1201 gacagccaca gcgcgagtcg ggtcagggca tagtttctga tctggccgag cagcagggcg
     1261 gaattcccat agcggaagta ccctgcccac ccgcggagga acaggttcag ttcctccacg
     1321 atccgctcga ccggtatcag cagccgggac cgggcggtaa tggaccgaat tcgatcccgg
     1381 gcgtgctggg ctgccttacg tgctggccag cgggcgagga agaccaaatg cgccgaccgg
     1441 ggagtccgcc cgcgcaccag tcggttatgg aagcccagga agtccaaccc ctgtcctccc
     1501 tcgacgaggc gcacgatgcg ggtcttggag ccttgggttc cagccccaaa gcgcccagca
     1561 agaccgtcac ccgcgctaag gcggcgtcgg cttgttccgt gactggcaca tcaccaccag
     1621 gtcatcgcag tagcgcacga gtacgccgtg ttcgcgggtg tcccatgccc ggtccagccg
     1681 gtgcaggtac acgttgcata acaacggcga tattggaccg ccttgcgggg ttccggatac
     1741 ctcgcggcgc actgccccat cggccatcac tcccgcacgc agcatcgcac gcaacagttt
     1801 catgattccc tggtcgctga tgcgttcttc gaccgcttgc atcaacttct catgcggaat
     1861 cgccgagaaa caattggcga tgtccgtttc caccacccac cgtgcaccct gccatgactc
     1921 atcaataagg acttgcaggg catcgtgcgc cgagcgtttc ggacgaaacc cgaaactaca
     1981 cgacagaaaa tccgcctcga agatcggctc gagcacgatc ttcaccgcag cctgcacgat
     2041 acggtcggca acccgaggaa tcgacaacgg acgtcgttca ctcgtcccag gcttgggaat
     2101 caacacccgc cgggacggca acggccgcca ctgaccggtc ctcagatcct cggccaactc
     2161 atccaagagt cggtcaacgc cgtactcctc gacatcggcc agggtggttc catcgattcc
     2221 cggtgcgcca gcgttgcgac gcaccgcgac ccacgcccgc cacaagacgt ctctgcgata
     2281 gaccttgtcc cgcagcgcat gaaaccctcg tcccggatcg gccttggccg accggtacag
     2341 cgcatgctgc aaggcacgaa ccagatccct ccgggatccc accgcggtgg aactagcctg
     2401 atcggcactc accgggcccc tcctcccaga caccacgcat gaacgaagca gcggcccttc
     2461 cctcaccggc ggttctgttg tccacccggc tcgatcagta ctacggccgc ctccgactcc
     2521 ctcccggcac ccgatccact tcccggctcc acaccggtta taagacgggc cctccgcgct
     2581 gctcgcagca cgcttgggcc ggggagggcc tccccagttc ccgccgccac cttctgaacg
     2641 ttccgcgccc tcttacgccg gggagtccct cgcggctgca cgttcaggat cttcaccgct
     2701 ccatggcctt caccgtgaac cgcccggctc ggctctcccc caatgtctaa cgacgcggca
     2761 ggcttcgctt ccgctacgga ccgctcagtt gcttccccca acagggactt tcgacgctgg
     2821 gcttcaaccc ggcccgtttc cagaccaggc cgccagcctg ctaccgggcc tcctgacagc
     2881 tacccggacc ggactcac
//
LOCUS       DATADN010001225         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_138639, whole genome shotgun sequence.
ACCESSION   DATADN010001225 DATADN010000000
VERSION     DATADN010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138639"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..367)
                     /locus_tag="VHH53_15690"
     CDS             complement(197..367)
                     /locus_tag="VHH53_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291589.1"
                     /translation="MTDPEATQQDAEVDVKPSADKPPPKQADYHEPAEETVTADYQTR
                     VRQLDEDTDQSG"
     gene            complement(1160..>1930)
                     /locus_tag="VHH53_15695"
     CDS             complement(1160..>1930)
                     /locus_tag="VHH53_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291590.1"
                     /translation="GPGADARQEDDAAVLTAVLFGLAASSGLVIGALFGARWSPPKQV
                     TGVLLAFASGALISALAFELFEEAFTMGGGVRAGLGLLAGAATFVAVDTALDRYVSGK
                     PGPEQREVVAFGARSGVGLALLAAVTLDGVPENLALGVSLVSGASLSLLVAIFFSNLP
                     ESLVGAVAMRESGLRSRTVVLTWVACAVLLAAAVVLGRGAASGLSEHVLAVALAFAGG
                     AVLASLADTLMPEAFEHGRPLNAFATAAGFFLSFILAG"
BASE COUNT          344 a          670 c          592 g          324 t
ORIGIN      
        1 gccgcatccc ggattctttg agggttcgtt atcgctgggc gccaaaatcg tccgaggtgg
       61 tcgcgagggg cgctggtcgc ggctacaccg gcgtaaacgg actcaacgcg ctacttgcca
      121 cgctgtccac gcactgtccg caccgatgat cgcggcacgg aacgccccac gaccgcaccc
      181 ctgcgcatca tcaccgtcag ccgctttgat ctgtgtcctc gtccaactgg cgcacacgcg
      241 tttggtagtc agcggtgact gtctcttccg ctggctcgtg gtaatcagct tgcttggggg
      301 gtggcttgtc ggctgaaggc ttgacatcca cttcggcgtc ctgctgtgtg gcttcaggat
      361 cagtcatcac caactcctta accgatgttc ggcagttgcc cactctgtac cactcacgga
      421 tgatcataaa catggaggtc tacagggtcg ggctgaagcg tcccgggacc ttcgcactat
      481 cattcgctgg tgttaccagc gagtcgttga gccactccag cgtcggatcc tgaatgctcc
      541 ggggtcagcc acagcgctcc ggcaccgtcc agagcggatc ccggaattct cgacgatgct
      601 caccgcagca tcgggggatt ccgtgacata aagctcgccg ggcgtcaccg tgtccagccg
      661 cacgcccggg cccgagggag gctcgaacag gcgttgctgt agatggtgat cttcgcagcg
      721 gatgtgaaga gcgccccagt atgggctgca acggcagcct cgagtcgcgc ggtacagggc
      781 gtgggtggtg cctgtagtag ataatctgcc cggtgtaggc ggttcgtcga ggatgtccgc
      841 ggtgagtggc gcggtgctgt ccctgttgcg cgatgcatac cagcgccgcg acaagattgg
      901 ccgtcgctgg aacgcgggcg gtatgactgg ccgtggcggg cgacccatac cttcccatcc
      961 ccgatcttgc cggttcgggc agggaatcta cgtggctttg gccccagcag ccgcagaggc
     1021 ttccccagct ccgcaactgc tattcctggc ggcccgcagc agcgctcatg ccgctgaccg
     1081 cgcggtgtcc gcacatgccg agaagcgtgc gtgctcggtg tcgttcgcct gcttaacaag
     1141 ctgacccttt ttgcattgat cagcccgcga gtatgaacga cagaaagaat cccgcagcgg
     1201 tagcgaacgc gttgagcggc cggccgtgct cgaacgcctc gggcatgagc gtgtcggcga
     1261 gcgaggccag caccgctccc cccgcgaacg cgagcgccac cgccaggaca tgctcgctca
     1321 acccgctcgc cgcgccccgg cccagcacga ccgccgcggc caacagcacc gcacaagcaa
     1381 cccacgtcaa caccacagtg cgcgaacgca gccccgactc acgcatagcg acggcaccca
     1441 ccagcgactc cggcaggttg gagaagaaga tcgccactag cagggatagt gacgcgccgc
     1501 taactagcga tactccaagc gcgaggttct ccggcactcc gtccagcgtc actgctgcca
     1561 gcagcgccag gcccaccccg ctacgggcgc cgaaggcaac gacctcgcgt tgctccggcc
     1621 cgggctttcc gctgacgtag cggtccaggg ccgtgtccac ggcaacgaac gtcgctgccc
     1681 ccgcgagcag cccgagccca gcgcgcaccc caccgcccat cgtgaacgct tcctcgaaca
     1741 gctcaaacgc cagcgccgaa atgagtgcgc cgctggcaaa cgccagcagc actcccgtca
     1801 cttgctttgg cgggctccac cgcgcgccga agagggcacc gatcaccagg ccgctggacg
     1861 cggcgagccc aaacaacacg gcggtgagca cggcggcatc atcctcctgt cgagcatcgg
     1921 cccctgggcc
//
LOCUS       DATADN010001226         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1348777, whole genome shotgun sequence.
ACCESSION   DATADN010001226 DATADN010000000
VERSION     DATADN010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348777"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1500)
                     /locus_tag="VHH53_15700"
     CDS             complement(<1..>1500)
                     /locus_tag="VHH53_15700"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2291591.1"
                     /translation="NVPGVANANVNLATERATVAYDPAVTDLTTLRGAVERAGYRVGE
                     IPAVDAAPAPQQAASRPAPPVTTVDPRERERDRELADLKHKWVVSLAIGLVMMAEMYL
                     PLGWDMTLVAPLLLIQATLVQFWAGGVFYRTAWAAARHGGTNMSTLVAVGTSAAYGYS
                     AFVTLWPQLAARWGFPFHLYYEVAVIVIALILLGRWLEARAKKQTGAAIKALMGLQAR
                     TARVIRNGVEQDIPVEQVVVGDLVRVRPGEKVPVDGVVTDGRSALDESMLTGESLPVE
                     KDPGDTVIGATLNKTGSFVFRATKVGRDTTLAQIVRLVEQAQGSKAPMQRLADTISSY
                     FVPVVLVLAALTFTTWLIFGPSPVFALTATIAVLIIACPCALGLATPTAIMVGTGKAA
                     EYGILVRGGEALEMTRKIDTIVLDKTGTLTRGKPAVTAVVPADGLTDRELLRLAAAAE
                     VGSEHPLGEAIVIRARELGADLPPVASFRSVTGKGIEAEVEGRAVAIGN"
BASE COUNT          250 a          519 c          490 g          241 t
ORIGIN      
        1 ggttgccgat ggcgactgcc cgcccctcaa cctcggcctc gatccctttg ccggtgaccg
       61 aacggaacga tgcgaccggc ggcagatcgg cccccagctc gcgggcgcgg atcacgatcg
      121 cctcaccgag cgggtgctcc gacccgacct ccgccgcggc agccagacgc aacagctccc
      181 ggtcggtgag tccgtcggcg ggcacgaccg cggtcaccgc cggcttgccg cgggtcagtg
      241 tcccggtctt gtcgaggaca atcgtgtcga tcttgcgcgt catctccagc gcctcaccgc
      301 cgcggacgag gatgccgtac tcggccgcct ttccggtgcc aaccatgatt gcggtcggcg
      361 tggccaagcc cagagcgcag gggcaggcga tgatcaggac cgcgatcgtg gccgtcagtg
      421 cgaagaccgg ggacggtccg aagattaacc aggtggtgaa cgtcagcgcc gccagcacca
      481 gcacaaccgg cacgaagtag ctcgagatgg tgtccgcgag ccgctgcatc ggggcttttg
      541 agccctgcgc ctgctccacc agacgaacga tctgggccag tgtcgtgtcg cgcccgacct
      601 tggtagcccg gaagacgaag ctgccggtct tgttgagcgt cgcgccgata acggtgtctc
      661 cggggtcctt ctccaccggg aggctctctc cggtcagcat gctctcatcg agcgccgaac
      721 gaccatcggt cacgacaccg tcgactggca ccttttcgcc ggggcgcacc cggacgagat
      781 cgccgacaac cacctgttcg acgggaatgt cctgctcgac gccgttgcgg atcacgcgag
      841 cggtcctagc ctgaaggccc atcaacgcct tgatcgcggc tccggtctgc ttcttggccc
      901 gcgcctccag ccagcgaccg agcagaatga gggcgatcac gatcacagcc acctcgtagt
      961 agaggtggaa tgggaaaccc cagcgggcgg ccagctgcgg ccagagggtg acgaaggcac
     1021 tgtagccata ggcggcgctg gttccaacgg ccaccagcgt gctcatattc gtgccgccat
     1081 ggcgtgccgc cgcccaggct gttctgtaga agaccccacc tgcccagaac tggaccagcg
     1141 tcgcctggat cagcagcaat ggcgccacca gcgtcatatc ccacccgagc ggtaggtaca
     1201 tttccgccat catcaccaga ccgatcgcca ggctgacgac ccatttgtgc ttgaggtcag
     1261 ccagctcgcg gtcacgctcc cgctcccgcg ggtcaaccgt ggtaactggt ggagcggggc
     1321 gtgacgccgc ctgttgcgga gcaggcgccg cgtcgacagc cgggatctcg ccgacgcggt
     1381 agccggcgcg ctcgaccgcg ccgcgcaggg tggtcagatc ggtgacggcc gggtcgtagg
     1441 caaccgtggc ccgctccgta gccaggttca cgttggcgtt ggcaacgccg gggacgtttt
//
LOCUS       DATADN010001227         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_296523, whole genome shotgun sequence.
ACCESSION   DATADN010001227 DATADN010000000
VERSION     DATADN010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296523"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VHH53_15705"
     CDS             <1..555
                     /locus_tag="VHH53_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX2291592.1"
                     /translation="VARLLGLGFPGGPYIDKAARAGGDPGSVRFPRGLSGPHDPPYDF
                     SFSGLKTAVARHVEELQRRGITVPVADVAAAFQEAVVDVLTAKAIRAATDRGIDILII
                     GGGVAANSRLRALAQQRCTAAGLRLRVPRPRLCTDNGAMVAALGAHLLAAGAPPSQLD
                     IPADPGLPITTSLVGSSSSTANRG"
     gene            824..1150
                     /gene="groES"
                     /locus_tag="VHH53_15710"
     CDS             824..1150
                     /gene="groES"
                     /locus_tag="VHH53_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX2291593.1"
                     /translation="MKTPESGGHTVASVNIKPLEDKIVVQASEAETTTASGIVIPDTA
                     KEKPQEGKVLAVGPGRINDSGTRIPVDVSVGDVVLYSKYGGTEIKHNGEEYLILSARD
                     VLAVVN"
     gene            1273..1610
                     /locus_tag="VHH53_15715"
                     /pseudo
     CDS             1273..1610
                     /locus_tag="VHH53_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943352.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
BASE COUNT          275 a          550 c          526 g          259 t
ORIGIN      
        1 gtcgcacgac tgctcggcct gggttttccc ggcgggccct acatcgacaa ggcggcccgg
       61 gccggcgggg accccgggag tgtccgcttt ccccgcggcc tgtccgggcc gcacgacccg
      121 ccctacgact tctccttctc cgggctcaag actgcggttg cccggcacgt cgaggagctg
      181 cagcgccgag gtatcacggt accggtggcc gacgtcgcgg cagctttcca ggaggccgtg
      241 gtggacgtgc tcaccgccaa ggcgatccgg gccgcgaccg atcggggcat cgacatcctg
      301 atcatcggcg gcggggtggc cgccaactca cggttgcgcg cgctggcaca gcaacgctgc
      361 acggcggccg ggttgcggct gcgggtgccc cggccacggc tgtgcaccga caacggagcc
      421 atggtggcgg cgctcggcgc gcacctgctc gccgccgggg ctcccccgtc ccagcttgac
      481 atccccgccg acccgggcct tccgatcacc acctccctgg tcggcagctc ttcaagtact
      541 gccaaccggg ggtgacggtg ctgttaactg ctaccaacct cgtgccggtc tgcgcggggt
      601 tggtagcggt ttgaggtcat gccgacccag gttggtgcta cttgaagagc taccaacggg
      661 aacacattgc gacttccggc caccctagtt gagtgctggc actcggcgca gtagtctgcc
      721 aattggcact ctggtcctca gagtgccaga catggcatgg gccacctcga cacccgcgac
      781 ggcgaggtgg ttgcagcctg catcagacaa ccctgccagc tctttgaaga ccccagaaag
      841 tggaggtcac accgtggcga gcgtgaacat caagccgctc gaggacaaga tcgtcgttca
      901 ggccagcgag gctgagacga cgaccgcgtc cggcatcgtt atcccggaca ccgccaagga
      961 gaagccccag gagggcaagg tcttggccgt gggcccgggt cggatcaatg acagcggcac
     1021 tcgcatcccg gtggatgtga gtgtcggtga tgtggtcctc tacagcaagt atggcggcac
     1081 cgagatcaag cacaacggcg aggagtacct cattctctcc gcccgcgacg tgctcgctgt
     1141 tgtcaactga cggctcgacc gtcatcaccg tggccgcccc gctcccctct tcaagggtca
     1201 gcggggcggt cccgcattgg gtcggtggca cggcagcgac ctaacagact tcgcttgtga
     1261 ggaaggcagg caatgcccaa gcagatcacg ttcgacgaga ccgcgcgcca agcgctggag
     1321 cgctgagtcg gccagctcgc cgccgcggtc aagatcactc tcggtccccg cggccggcac
     1381 gtggtgctcg accgcaagta cggcggcccg tcggtcacca acgacggcgt cacgatcgca
     1441 cgcgagatcg agctgaccga tccctatgag aacctcggcg cccagcttgc caagagcgtg
     1501 gcgacgaaga ccaacgatgt cgctggtgat ggcaccacca ccgccaccgt gctcgcgcag
     1561 gccatggtta gtctgggact gcgaaatgtg gccgccggtg ccaaccccac
//
LOCUS       DATADN010001228         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_297265, whole genome shotgun sequence.
ACCESSION   DATADN010001228 DATADN010000000
VERSION     DATADN010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297265"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VHH53_15720"
     CDS             complement(<1..641)
                     /locus_tag="VHH53_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2291594.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKSLESLGISLEGVRSQVEEIIGQGQQAPSGHIPFTRRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLNRVRQQVIQLLSGYAGGEPGQGAKAGVGP
                     GGSPEEGKGSPVLDQFGRNLTQLAREGKLDPVIGRTREIERVMQVLSRRTKNNP"
     gene            complement(905..1246)
                     /locus_tag="VHH53_15725"
     CDS             complement(905..1246)
                     /locus_tag="VHH53_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEX2291595.1"
                     /translation="MAKKVTVTLVDDFDGEGAADETVEFGLDGVSYEIDLSSKNAAKL
                     RSDLKQWVEAGRRVGGRRRGRSAGSGRGRAAIDREQSAAIREWARRNGHNVSTRGRIP
                     ADVIDAFHAAT"
     gene            complement(1361..>1730)
                     /locus_tag="VHH53_15730"
     CDS             complement(1361..>1730)
                     /locus_tag="VHH53_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX2291596.1"
                     /translation="LWAPTFVRDFPVETTPLTRPHRSIPGVTEKWDLYIRRFELATGY
                     SELIDPVIQRERFEAQARAAAAGDDEAMALDEDFLAALEYAMPPSTGTGMGIDRLLMA
                     LTGLSIRETVLFPIVRRHAN"
BASE COUNT          289 a          582 c          539 g          320 t
ORIGIN      
        1 ggattgttct tggtgcggcg cgacagcacc tgcatgaccc gttcgatttc gcgggtgcgg
       61 ccgatgacgg ggtcaagctt gccctcgcga gccaactgcg taaggttgcg gccgaactgg
      121 tcgaggaccg ggctgccctt gccctcctcg ggtgagccac cgggaccgac acccgccttg
      181 gcgccttgtc cgggctctcc gcccgcgtag ccgctcagca gctggatgac ctgctggcgc
      241 acacggttga ggtcggcgcc gagcttctgc agcacctgcg cggcgacccc ctcaccttcg
      301 cggatgaggc cgagcaggat gtgctcggtg ccgatgtagt tgtggccgag ctgcagcgcc
      361 tcgcgcaggc tcagctccag caccttcttg gcgcgccggg tgaacgggat gtggcccgac
      421 ggcgcctgct ggccctggcc gatgatctcc tcgacctggc tgcgcacgcc ctcgagcgag
      481 atgcccagcg actccagcga cttggcggct acgccttcac cctcgtgaat aagtcccaac
      541 aggatgtgct cggtgccgat gtaattgtgg ttgagcatcc gggcctcttc ttgggccagg
      601 acgacgaccc tgcgggcacg gtcggtgaat ctctcgaaca tcggtggtta cctgctctcc
      661 atcaataggt cggtgccatg cgtgcccagg tgtctcgagt aacgcaccgc acctgccgtc
      721 cactctaatg ggcggcgctc cccagcgccc cgcctgccgg tctgctgtca gcggacacac
      781 tgggcatcaa cggcatcggg tggaataccc gacgctaccg attagcaacg cgtcggcggg
      841 gtgattcgtt tcccgatcga gccgcagcag gttcgcggct agcgaacggg gactgaggac
      901 cggtctaggt ggccgcgtgg aatgcgtcga tcacgtcggc gggaatgcgg ccgcgcgtcg
      961 acacattgtg tccgttgcgg cgggcccact cccggatcgc ggcgctctgc tcacggtcga
     1021 tggccgcgcg cccgcggccg gaccccgcgg agcggccgcg ccggcgaccg ccgacccggc
     1081 gaccagcctc gacccactgc ttgaggtcgc tgcgcagctt cgcggcattc ttagaagaaa
     1141 ggtcgatctc gtagctcaca ccgtcgaggc cgaattcaac cgtctcgtca gccgcacctt
     1201 cgccgtcgaa atcatcgacc agcgtgacgg taactttctt cgccatttag acacacgccc
     1261 ttctgcacga tcttcacgat cgcagttaag tcctcgaacc cccgcaccaa tgtgccatat
     1321 ttgcgtgccc cattcaacaa ggccggaata gccccctgcg tcagttggcg tggcgccgaa
     1381 caatcgggaa caaaaccgtc tccctaatcg acagtcccgt caaagccatc aacaatctat
     1441 cgatgcccat tccggtgccc gtggacggcg gcatcgcata ctccagggcg gccagaaaat
     1501 cctcgtcaag agccattgcc tcgtcgtcac cggcggcagc cgcccgggct tgtgcttcga
     1561 acctttcgcg ttggattacc gggtcgatca gttcggaata accggtggcc agttcgaagc
     1621 gccggatgta gagatcccat ttctcggtga cccccggaat actgcgatgt ggacgggtca
     1681 gcggagtcgt ctcgacgggg aagtcgcgga cgaaggtggg cgcccacagc
//
LOCUS       DATADN010001229         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_898669, whole genome shotgun sequence.
ACCESSION   DATADN010001229 DATADN010000000
VERSION     DATADN010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898669"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1052
                     /locus_tag="VHH53_15735"
     CDS             183..1052
                     /locus_tag="VHH53_15735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX2291597.1"
                     /translation="MAQRPGSPRLRDRPYLIACALAGAVVMVLAIVVVAFLVPLGGGG
                     SGEAGKAVARPPGTATTTIPPAAVPSDLAALPLSTTYTIVDGAPLDTGPPVPTDGTVV
                     HPRREVVVYAAPGGAPIARMTPQQLGDTWLPVIAEQPGWVQVLLPSRPNDSVGWVTDD
                     ALDRAVTPYLIRVHLRSLNMELFWGDRRLGEWPVGIGKESAPTPVGRTFLLGSFTDPK
                     QSYSPVILPLGTHSPTLDSFGGGPGTVAIHTWPTADVFGTRSSDGCIRVPADALNMLT
                     EVPLGTLVLIDEN"
     gene            1072..>1889
                     /locus_tag="VHH53_15740"
     CDS             1072..>1889
                     /locus_tag="VHH53_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291598.1"
                     /translation="MNSRKLAGASAASVGAATAVVALLLGTVSGNADEATKSSAYGIE
                     IVDLIDPTPFVDAPPDDSEALLEVPGEDSELLRVALLKVRAQDFRSKATAVDVEVLGI
                     EAKLIEAKCENGEGDSSIIGGVSDNGTPLPANPSEGQELDLSPILSIEFNRQDKDGDK
                     LTVDALVVKLLPGGNLGAVLQQKDLDLLKKVAPKDLKVPATMQEVKSENPSQPGAPAN
                     PSQPGAPANPAESGPVTLGDLTEKIQSLNPNLVPKADGDDDALLEVIVSSASCR"
BASE COUNT          328 a          614 c          632 g          315 t
ORIGIN      
        1 cacggagatc aaccgcagcg acgacaccct ctagagggcc cacggagggc ccctcaaagg
       61 cgtcggctcg gatccgttag ggtcccgtca ccgcgcggcc ggagcgtcgt tgggccagcg
      121 tgggtcgccc ggtcggggag ggcgagtcag caaggtccgc ccgtccgaca ccagaagagg
      181 ctatggctca gcgtcctgga tctccccgcc tgcgtgaccg tccttacctg attgcctgcg
      241 cgcttgccgg ggccgtcgtg atggtgctgg cgatcgtggt ggtggccttc ctggttccgc
      301 tggggggcgg tgggagtggc gaggccggca aggccgtggc acggcccccg gggaccgcga
      361 ccaccaccat cccgcccgct gccgtcccgt cagatctcgc agctctgccg ctgagtacca
      421 cctacaccat cgtcgatggg gcaccgctgg acactgggcc cccggtcccc accgacggga
      481 ccgtggtgca cccacggcgg gaagtcgtgg tatacgccgc ccccggcggt gcgccgatcg
      541 ccaggatgac cccccagcag ctcggtgaca cctggttgcc ggtgatcgcc gagcaacccg
      601 gctgggtgca ggtgctactg ccctcgcgac ccaacgattc ggtcggctgg gtaaccgacg
      661 atgcactcga tcgtgcggta actccgtatc tcatccgggt acaccttcgt tccctcaata
      721 tggagctgtt ctggggggac cggcggctgg gtgagtggcc ggtggggatc ggcaaggagt
      781 cagcgcccac tccggtgggg cggaccttcc tgctcgggtc gttcaccgat cccaagcaaa
      841 gctattcccc ggtgatcctg ccgctgggca cccacagccc aacgctggac agttttggtg
      901 gcggcccggg aaccgtcgcg atccacacct ggcccacggc cgacgtcttc ggtacccgct
      961 ccagcgatgg atgcatccgg gttccggcgg acgcgctgaa catgttgacc gaggttccgc
     1021 tgggaacgct cgtgctcatt gacgagaact gaacaaatga gaggacagca cgtgaacagt
     1081 aggaagctcg ccggcgccag tgctgccagc gtcggtgctg ccaccgccgt ggtggccctg
     1141 ctgctgggga ccgtgtccgg taatgctgat gaagccacca agtcatcggc gtatggcatc
     1201 gagatcgtag acctcattga tccgacgcca tttgtcgacg ctccgccgga cgacagtgag
     1261 gccctcctgg aggtgccagg agaggactcg gaactgctta gggtcgcgct gctcaaggtc
     1321 agggcccagg acttccggtc caaggcgacg gctgtcgacg tggaggtcct cgggatcgag
     1381 gccaagctca tcgaggcaaa gtgcgagaac ggtgaagggg attcctcgat catcggcggg
     1441 gtcagcgaca acggtactcc gctaccggcg aatccatccg aaggtcagga gctggacctc
     1501 tcgcccatcc tctcgatcga gttcaaccgg caggacaagg atggcgacaa gctcaccgtt
     1561 gatgcgctcg tggtgaagct cctgcccggt ggcaacctcg gggctgtgct ccagcagaag
     1621 gacctcgatc tcctcaagaa ggtcgcgccc aaggacctga aggtcccggc gacgatgcaa
     1681 gaggtgaagt cggagaaccc gtcgcaacca ggtgctccgg ctaacccgtc gcaacctggt
     1741 gctccggcca acccggcgga gtcgggcccg gtgaccctgg gagacttgac cgagaagatc
     1801 cagtcgctca acccgaacct ggtgcctaag gccgacggtg acgatgatgc gctgctagag
     1861 gtcatcgtat cgtctgccag ctgccggga
//
LOCUS       DATADN010001230         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_297618, whole genome shotgun sequence.
ACCESSION   DATADN010001230 DATADN010000000
VERSION     DATADN010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297618"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VHH53_15745"
     CDS             complement(<1..917)
                     /locus_tag="VHH53_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2291599.1"
                     /translation="MTTARAGGCLLALLGTTALAAPLLAPYDPNARTGTPFARPSAAH
                     LLGTNDVGHDLLSELIYGARISLLIGIVAALAATLVGLAVGVTAGYARGWLDTILMRL
                     VDVVLALPILPLALVIGVFLGPGLGNQILVITLVIWAPVARELRAQVLSLRERDHIQA
                     LRAMGGGAGYVLSRHVIPAVAPLVVPQFVLGTKNAILLESSLAFLGLGDVTAKSWGTM
                     LSLAHSRSAFLTDAWLWWVIPPGVAIAATVLAFALVGYAFEERAKPTLRDRPSRQPDR
                     RGTAVTSSTTTVPRTAGNPLTIINLTVSYG"
     gene            complement(914..>1719)
                     /locus_tag="VHH53_15750"
     CDS             complement(914..>1719)
                     /locus_tag="VHH53_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2291600.1"
                     /translation="LDRPILEQYAGFWTGLARGDLGISVAHNRPVVDLLLERLPWTLA
                     LIGLGILGATMLGVALGAAAAWWRGRRRDVGLVAGVLAVDSMPSFWIGMMLIAVFAVQ
                     LGWFPSFGATEITAGGAAAIGEMLRRLVLPVVTITLAIFGSIFLLTRAAMVSVLDEPF
                     VRLARAKGVTERRIALRHALRNALLPVFTNVTVMVGTLLSGLVVVETVFAYPGLGRLI
                     YEAVTARDYPLLQGGFLLITIGVIVLNLLADLTYPLLDPRVRRPAESAL"
BASE COUNT          327 a          612 c          569 g          211 t
ORIGIN      
        1 ccgtagctga cagtgaggtt gatgatcgtc agcgggttgc ccgcggtcct cggcaccgtc
       61 gtcgtcgagg acgtcaccgc tgtcccgcgg cggtctggtt gccggctcgg ccggtcgcgc
      121 agcgtgggct tggcccgctc ctcgaaggcg taacccacca atgcgaaggc gagcaccgtg
      181 gcggcgatcg ccacgccggg tgggatcacc caccacaacc acgcatcggt caaaaaggcg
      241 ctgcgggaat gtgccagcga cagcatcgtg ccccagctct tggccgtcac gtcgcccaga
      301 ccgaggaacg ccagcgacga ttcgagcagg atggcgttct tggttccgag cacgaactgc
      361 ggcacgacca ggggcgcgac cgcgggaatg acgtggcgcg acagcacgta acccgcgccg
      421 ccgcccatcg cgcgcaacgc ctggatgtgg tcgcgttcgc gcaacgacag cacctgagcg
      481 cgcagctcgc gcgccaccgg agcccagatc accagtgtga tcaccaggat ctggttcccc
      541 agcccgggac cgagaaagac cccgatcacc agcgccagcg gcagtatcgg cagggccagc
      601 acgacatcga ccaggcgcat caggatggtg tccaaccagc cgcgggcata cccggccgtc
      661 accccgacgg ccaagccgac cagcgtcgcc gccagtgcgg ccacgatccc gatcagcagc
      721 gagatgcgcg cgccatagat cagctcggag agcaggtcgt ggcccacgtc attggtaccg
      781 agcaggtgcg cggccgaggg acgggcgaac ggggtaccgg tccgcgcgtt cgggtcatac
      841 ggcgccagta acggcgcggc cagggcggtg gttcccagca acgcgagcag gcatcccccc
      901 gcccgggcag tggtcataac gccgactccg cgggacggcg tacccgcggg tccagcaggg
      961 gataggtcag atcggcgagc aggttcagca cgatgacacc gatggtgatc aacaagaacc
     1021 cgccttgcag caacgggtag tcccgggcgg tcactgcctc gtagatcagc cggcccagtc
     1081 ccgggtaggc gaacaccgtc tcgactacga ccaggcctga cagcagcgtt cccaccatca
     1141 ccgtcacatt ggtgaagacc ggcagcagcg cgttgcgcag tgcatggcgc agcgcgatcc
     1201 ggcgctcggt tacccctttc gcccgagcca accggacgaa cggctcgtcg agcactgaga
     1261 ccatcgccgc ccgggtgagc aggaagatcg aaccgaagat ggcgagcgtg atcgtgacca
     1321 ctggcagcac cagccggcgc agcatctccc cgatcgccgc cgccccgccc gcggtgatct
     1381 cagtggcgcc gaaggaggga aaccagccga gctgcaccgc gaaaaccgcg atcagcatca
     1441 tgccgatcca aaaactcggc atcgaatcga ctgccagcac accggcgacc agcccgacgt
     1501 cgcgccggcg gccccgccac caggccgctg ccgccccgag tgcgacgccg agcatcgtgg
     1561 cacccaggat cccgaggccg atcagcgcca gggtccacgg caggcgttcg aggagcagat
     1621 cgaccaccgg gcggttgtgc gcgactgaga tgccgaggtc gccgcgcgcc agcccggtcc
     1681 agaacccggc gtactgctcc aggatcggcc ggtccagcc
//
LOCUS       DATADN010001231         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_141310, whole genome shotgun sequence.
ACCESSION   DATADN010001231 DATADN010000000
VERSION     DATADN010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141310"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VHH53_15755"
     CDS             <1..358
                     /locus_tag="VHH53_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX2291601.1"
                     /translation="AQEAFRPDSVGAQLIEEMSTLAQCDRVVDNQKLSGLAGNGLPDW
                     LAERGIDTVFVTGVATNLVVEQTARNGTDLGLTVHAVSDCVVAADEAVHFASLANLDL
                     TTAGCLTAEQALGLLR"
     gene            complement(401..817)
                     /locus_tag="VHH53_15760"
     CDS             complement(401..817)
                     /locus_tag="VHH53_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2291602.1"
                     /translation="MATDHITRNKQLVEAFIQELFSKGDLDAVDRYLDPGFVNHDPPF
                     PGAPDGPDSMRQVGAMFREALPDWHSDVDQFVAEGDIVVERFTASGTHRGELMGVPPT
                     GKTIVLRGINLFRIAGDRIVERWGRLDEVGCCVNSG"
     gene            complement(820..990)
                     /locus_tag="VHH53_15765"
     CDS             complement(820..990)
                     /locus_tag="VHH53_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291603.1"
                     /translation="MSQPAYVQAAVAVADHVALSLDQAGLPRHDHSVLLVEQSRNPSF
                     LGWSYVSAGYTI"
     gene            994..>1774
                     /locus_tag="VHH53_15770"
     CDS             994..>1774
                     /locus_tag="VHH53_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2291604.1"
                     /translation="MLQSLRRLMPVDAAFFATADPMTLLFTGAHAEEPLSAATPLFLD
                     NEFASNDVNKFALLATSTTHVASLDGATRRDRSSSPRYRDIMRPLGLGDELRAALITG
                     SECWGYLCLHRADHPLGFTHSEAALIARLGPHIAHALRQAVLLHRSPTAGNAPRPGVV
                     LLARDLSLVASTPEADHLLSLLEDDRSIGLPLPVVVYTVAGALGAVEHGAAPSDVLPS
                     ARVRTTVGRWLNVHASRLQGPPGENRIAVVVEPAEPRATLSI"
BASE COUNT          320 a          598 c          549 g          307 t
ORIGIN      
        1 tgcccaggaa gcattccgcc cggattccgt cggcgcgcag ttgatcgagg agatgtcgac
       61 gctggcccag tgcgatcgag tcgtcgataa ccagaagctg tccggacttg cgggtaacgg
      121 cctccccgac tggttggctg agcgcgggat cgacaccgta ttcgtgaccg gcgttgccac
      181 caacctggta gtggagcaga cggcccgaaa cggaaccgac ctcggcctca cagtgcacgc
      241 ggtcagcgat tgcgtcgtcg cggccgacga agccgttcac tttgcgtccc tggccaacct
      301 ggatctcacc actgcgggtt gcctgaccgc agagcaggcg cttgggttgc tccggtaacc
      361 acaaagcggg ggacccggtc agacccggcc tagccggaga tcaacccgag ttgacgcagc
      421 agcctacctc atcgaggcgg ccccaacgct cgacgatcct gtcgccggcg atccggaaga
      481 gattgatacc gcgcagcacg atcgtcttac ccgttggagg cacgcccatc agttcccccc
      541 ggtgcgtccc gctggcggtg aaccgctcga caacaatgtc gccctcggcc acgaattggt
      601 ccacgtcgct gtgccagtct ggcagcgcct cccggaacat ggcgccaacc tggcgcatgc
      661 tgtcaggtcc gtcaggcgcg ccggggaatg gcggatcgtg gttgacgaaa cccgggtcca
      721 ggtagcgatc gacagcatca agatcgccct tgctgaacag ctcctggatg aacgcctcaa
      781 cgagctgctt attgcgggtg atgtgatcag ttgccatgct cagatggtgt agccggcgct
      841 gacatacgac catcccagaa agctggggtt cctcgactgc tcgaccagca ggacactgtg
      901 gtcatggcga ggaaggccag cctggtccag gctgagcgcg acatggtccg ccactgccac
      961 agcggcctgg acgtaggcag gctgcgacac caggtgctgc aatcgctgcg gcggctgatg
     1021 ccggtggacg ccgcattctt cgccaccgct gaccccatga cgctgctgtt caccggcgca
     1081 catgccgagg aaccactcag cgcagcgacg cccttatttc tcgacaacga gttcgccagt
     1141 aatgatgtga acaagttcgc cttgctggcg acctcgacaa cgcacgtggc ctcgctcgac
     1201 ggtgcgacac gccgcgaccg ctcatcaagc ccgcgatacc gcgacatcat gaggccgctc
     1261 ggtctcggtg atgagctcag ggcggcgttg atcaccggct cggagtgctg gggttacctg
     1321 tgcctgcatc gggcggacca cccgctcggt ttcacgcact ccgaagcagc actgatcgcc
     1381 agactcggcc cccacatcgc ccacgcacta cgccaagccg tactgctgca ccgttctcca
     1441 acggccggca atgcccctcg gccaggcgtc gtgctcctcg cccgcgatct gagtctggtg
     1501 gcgagcacac cggaagcaga ccatctgctg tcgctgctcg aggatgatcg ttccatcggg
     1561 cttccgctac cggtggtcgt ctacaccgtc gcgggggcac tgggagcggt ggaacatgga
     1621 gcggcaccat ctgacgtctt gcctagcgca cgagtccgga ccaccgtagg acgttggctc
     1681 aatgtgcacg catcgcggct ccagggaccg cctggtgaga accgcatcgc ggtcgtcgtc
     1741 gaacccgccg agccgcgcgc gaccttgtca atcc
//
LOCUS       DATADN010001232         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_900298, whole genome shotgun sequence.
ACCESSION   DATADN010001232 DATADN010000000
VERSION     DATADN010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900298"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="VHH53_15775"
     CDS             <1..105
                     /locus_tag="VHH53_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX2291605.1"
                     /translation="VVGTIAGDDTILVVAREPLPGAKLAERIMALARS"
     gene            167..1372
                     /locus_tag="VHH53_15780"
     CDS             167..1372
                     /locus_tag="VHH53_15780"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX2291606.1"
                     /translation="MTERVVLAYSGGLDTSVAIGWIAEKTGAEVVAVAVDVGQGGEDL
                     ETIRKRALACGAVEAVVTDARNEYAEQYCLPALQANALYMDRYPLVSALSRPLIVKHL
                     VAAAKYHGAGTVAHGCTGKGNDQVRFEVGIGALAPDLQVIAPVRDYAWTREKAIAYAA
                     ERNLPIDVTKRSPFSIDQNMWGRAVETGFLEDLWNAPTKDVYSYTSDPTEWKAPEQVV
                     IAFESGMPVAIDGAPVTMLEAIQQLNERAGTHGVGRLDLVEDRLVGIKSREVYEVPGA
                     ITLLTAHQELENVTVERDLARYKRNVEQRWTELVYDGLWFSPLKRALDAFVAESQHHV
                     TGEIRMELAGGRATVTGRRSTQSLYDFNLATYDEGDTFDQSLAKGFVQLWGLPSKIAA
                     QRDLRGFPP"
     gene            1369..2769
                     /gene="argH"
                     /locus_tag="VHH53_15785"
     CDS             1369..2769
                     /gene="argH"
                     /locus_tag="VHH53_15785"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX2291607.1"
                     /translation="MTTLWGGRFSGKPADAMTALSLSTHFDWRLARYELRGSRAHARV
                     LHDAGLLNDGELDAMLDALDHLDADVSSGAFVPMPEDEDVHTALERGLIERAGPELGG
                     ALRAGRSRNDQVATLFRMWLRDAARRVAAGTLDVIDALLDQAQAHPDAPMPGRTHMQH
                     AQPVLLAHHLGAHVQALLRDVERLRDWDARAAVSPYGSGALAGSSLGLDPAAVAAELG
                     FGAPVENSIDGTASRDFAAEAAFVLAMLAVDLSRIAEEIILWATAEFGYVALDDAYST
                     GSSIMPQKKNPDVAELARGKAGRLIGNLTGLLATLKAQPLAYNRDLKEDKEPLFDSVD
                     QLELLLPAMAGMVGTLVFDTERLAELAPAGFALATDLAEWLVRQGVPFRSAHHAAGAC
                     VRLAEYRGVELSELSEMELTAIHPELTTDVHRVLSVAGSIGSRDAYGGTAPERVAEQL
                     QRARQAASEHRGWITS"
     gene            2800..3414
                     /locus_tag="VHH53_15790"
     CDS             2800..3414
                     /locus_tag="VHH53_15790"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2291608.1"
                     /translation="MTLETFERECLAVDVLPAARLLLGCHLVADRPQGQVAVRLVELE
                     AYRGSDDPAAHSYRGRTARNAVMFGPPGHLYVYFIYGLHFCANISCLPDGQAGAVLLR
                     AAEVVSDLEIARLHRPTARSDAELARGPAWLATVLGLRREHNGLDLTDPASPVRLLSG
                     DPVPAEMVRTGPRVGVAAAPDRPWRFWVHGSPAVSAYRRHRQPA"
     gene            3451..>3718
                     /locus_tag="VHH53_15795"
     CDS             3451..>3718
                     /locus_tag="VHH53_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX2291609.1"
                     /translation="MAEHILDELSWRGLIAQSTDLDALRRDCDAGPVTVYGGFDPTAP
                     SLHVGNLVLILTLRRFQRAGHRPLALVGGATGLIGDPKATAERTL"
BASE COUNT          589 a         1268 c         1273 g          588 t
ORIGIN      
        1 gtggtcggga ccattgccgg agatgacacc atcctggtgg tcgcccgcga acccctccct
       61 ggggcgaaac tcgcggagcg gatcatggcg ctggcgcgct cgtgagcgcg aatcggggct
      121 ataacactgt ttgccactca caagctcggc cggagggaga gcagggatga ctgagcgggt
      181 ggtgctggcc tactccggtg ggctggacac ctcggtggct atcgggtgga ttgctgagaa
      241 aaccggagct gaagtcgtcg cggtggcggt tgacgtcggc cagggcggcg aggacctgga
      301 gacgatccgc aagcgggcgc tggcctgcgg cgcggtggag gccgtcgtca ccgacgcccg
      361 caacgaatac gccgagcagt actgcctgcc ggcgctccag gccaacgcgc tctacatgga
      421 ccgctacccg ctggtatcgg cgctgtcccg gccattgatc gtcaagcatc tggtggcagc
      481 cgcgaagtac cacggtgccg gcaccgtcgc acatggctgc accggcaagg gcaacgacca
      541 ggtgcgcttc gaagtcggca tcggcgcgct ggccccggat ttgcaggtga tcgctccggt
      601 ccgggactac gcgtggaccc gggaaaaggc gatcgcctac gccgcggagc gcaacctgcc
      661 gatcgacgtc accaagcgct cgccgttctc gatcgaccag aacatgtggg gccgagcggt
      721 agagacggga ttcctcgaag acctgtggaa cgcccccacc aaggacgtgt actcctacac
      781 cagcgatccg accgagtgga aggcaccgga gcaggtggtg atcgctttcg agtcgggtat
      841 gccggtcgcg atcgacggcg cgcccgtcac catgctcgag gcgatccagc aactcaacga
      901 gcgggctgga acgcacggcg tcggccggct ggatctggtc gaggaccggt tggtcggcat
      961 caagagccgg gaagtctacg aggtgcccgg ggcgatcacc ttgctcaccg cacaccagga
     1021 gctggagaac gtcaccgtcg agcgagacct ggccaggtac aagcgcaacg tcgagcaacg
     1081 ctggaccgaa ctagtctacg acgggctctg gttctccccg ctcaagcgcg ccctggacgc
     1141 cttcgtcgcc gaatcccagc accacgtcac cggcgagatc cggatggagc tggccggcgg
     1201 ccgcgccacg gtgaccgggc ggcgcagcac gcagtcgctc tacgacttca acctggccac
     1261 ctacgacgag ggtgacacct tcgaccagtc gctggccaag gggttcgtgc aactgtgggg
     1321 gctgccctca aaaatcgccg cacaacgaga cctgcgaggc ttcccaccgt gaccacactt
     1381 tggggtggcc ggttcagcgg caaaccggcc gatgcgatga ctgcactgag cctgtcgaca
     1441 cacttcgatt ggcggctggc ccggtacgag ctgcgtggct cccgggcgca cgcccgggtg
     1501 ctgcacgatg ccgggctgct caacgacggt gagctggacg cgatgctcga cgcactggac
     1561 cacctcgacg ccgacgtcag ctccggcgcc tttgtcccga tgcccgagga tgaggacgtg
     1621 cacaccgctt tggagcgggg tctgatcgag cgcgccgggc cggaactcgg cggtgcattg
     1681 cgcgccggtc gctcccgcaa cgaccaggtc gcgacgttgt tccggatgtg gctacgcgac
     1741 gctgcccgcc gggtcgcggc cggcaccctg gacgtgatcg acgcgttgct cgaccaggcg
     1801 caggcgcatc cggacgcacc catgccgggt cgaacccata tgcagcacgc ccagcctgtg
     1861 ctgctcgccc accacctggg ggcgcatgtc caggccttgt tgcgcgatgt cgagcggctg
     1921 cgcgactggg atgcccgggc ggccgtctca ccctatggct cgggtgccct ggcgggttcg
     1981 tcgctgggcc tggacccggc agcagtggcc gccgaactcg gcttcggtgc cccggtggag
     2041 aactcgatcg acggcacggc atcgcgcgac ttcgccgccg aggccgcgtt cgtgcttgcg
     2101 atgctggccg tcgacctgtc ccggatcgcc gaggagatca tcctctgggc caccgccgag
     2161 ttcggttacg tcgcgctcga tgatgcctac tcgaccggta gctcgatcat gccgcaaaag
     2221 aagaaccctg atgtggccga attggctcgg gggaaggcag gccggttgat cggcaacctc
     2281 accgggctgt tggcgacgct caaggcgcag cccttggcat acaaccggga cctgaaagag
     2341 gacaaggagc cgctgttcga ctccgttgac cagctcgaat tgctgctgcc cgcgatggcc
     2401 ggaatggtcg gcaccctggt cttcgacacc gaacggctcg ccgagctggc tccggccgga
     2461 ttcgcgttgg ccacggacct cgccgaatgg ctggtgcgtc agggtgtgcc cttccgcagc
     2521 gcacaccatg cggctggggc ctgcgtacgg ctggccgaat accgcggggt agagctgtcc
     2581 gagctgtcgg agatggagct caccgccatc catcccgagc tcaccactga cgtccaccgg
     2641 gtgctcagcg tcgccggctc gatcggctcc cgggacgcgt acgggggcac tgcacctgaa
     2701 cgcgttgccg agcagttgca gcgcgcccgg caggcagcca gcgagcaccg gggctggatc
     2761 acctcgtgac ggtggggatg ttgtgacagc ggagacgctg tgacgctaga gacgttcgaa
     2821 cgcgaatgcc tcgctgtaga cgtcctgcca gctgctcggc tgctgctggg ctgccacctg
     2881 gttgctgacc gaccgcaggg ccaggtggcc gtccggctcg tcgagctgga ggcctatcgc
     2941 ggctccgatg atccggcggc gcactcctac cgcggccgca ccgcccgcaa cgccgtcatg
     3001 ttcggcccgc ccggacacct gtatgtgtat ttcatctacg gcttgcactt ctgcgccaac
     3061 atctcctgcc tgcccgatgg acaagccggt gcggtgttac tgcgcgccgc cgaggtggtg
     3121 tccgatctcg aaatcgcccg gctgcaccgg cccaccgcac ggtccgacgc cgagctcgct
     3181 cgcgggcccg cgtggctcgc gacggtgctc ggactgcgcc gcgagcacaa cggcctggac
     3241 ctcaccgacc cggcgtcacc ggtgcggctg ctgtccggcg atccggtgcc cgccgagatg
     3301 gtacgcaccg gtcctcgggt cggcgtcgcc gctgcgccgg accggccgtg gcggttctgg
     3361 gtgcacggta gcccggcggt gtcggcctac cgccgccacc ggcaacctgc ctgatccgca
     3421 acggatcatc cagtacggga tgatgcagtg gtggcagagc acatccttga cgagctgagc
     3481 tggcgcggtc tgatcgcgca gagcaccgac ctcgacgcgc tgcgccgcga ctgtgacgcg
     3541 ggcccggtga ccgtctacgg cggcttcgat cccaccgcgc caagcctgca cgtcggcaac
     3601 ctggtgctga tcctgacgct gcgccggttc cagcgggccg gccatcgccc cctcgcgctg
     3661 gtgggcggtg ccaccggcct catcggcgac ccgaaggcga ccgctgagcg caccctca
//
LOCUS       DATADN010001233         3706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1351996, whole genome shotgun sequence.
ACCESSION   DATADN010001233 DATADN010000000
VERSION     DATADN010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351996"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VHH53_15800"
     CDS             complement(<1..645)
                     /locus_tag="VHH53_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2291610.1"
                     /translation="MVRPRRGPGLRARLTLLATGLSAAVSALLLWLGWLLVGGVVAAV
                     PDLPPGSTVEVDGVPVAADQLGAALQEAARQRVLEVGSVAFLLAVTATALLAWVITGQ
                     VLRPLHDVTATARRLSAESLDERLRLPGPRDEVAELADTFDAMLDRLQAAFEAQRRFV
                     ANASHELRTPLAVLRTEVDVTLADPAADEAELRRMATVIRDATLRAQQLVDGLLV"
     gene            complement(650..1327)
                     /locus_tag="VHH53_15805"
     CDS             complement(650..1327)
                     /locus_tag="VHH53_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2291611.1"
                     /translation="MRILIVEDEQPLADAVARGLRREGMAVDVAYDGDSGHEKAAVTR
                     YDVVVLDRDLPGMSGDQLCAEITGSGALTRVLMLTASGTVSDRVAGLSLGADDYMAKP
                     FAFAELVARLRALGRRATPAVPPVLSAADVLLDPSRRTVSRSGIPIDLTRKEFGVLEV
                     LLSAGGGVVSSEELLERVWDEHADPFTTTVRVTMMTLRKKLGEPGIIETVVGSGYRVP
                     TTDSAPG"
     gene            complement(1373..3310)
                     /gene="dxs"
                     /locus_tag="VHH53_15810"
     CDS             complement(1373..3310)
                     /gene="dxs"
                     /locus_tag="VHH53_15810"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX2291612.1"
                     /translation="MTLLQSVRSPQDVKALDAEALTQLASEIRTFLVDKVSRTGGHLG
                     SNLGAVELTLAVHRVFDSPHDPVIFDTGHQAYVHKILTGRIEGFDRLRKRGGLSGYPC
                     RAESEHDWVESSHASAALSYADGLAKAFAHGASSPAASRHVVVVVGDGALTGGMCWEA
                     LNNIAAGQDRPVVIVVNDNGRSYSPTIGGLADHLSTLRLQPGYERVLRTGKQALQRTP
                     VVGRAIYAGLHAAKRGLKDALTPQILFEDLGLKYLGPVDGHDMQAMESALRRAKDFGG
                     PVIVHAVTRKGNGYPPAEKDAAELMHQVKVMDPETGVPIAPTGVSWTSVFTEELIRLA
                     GEREDLVAITAAMPGPTGLAAFAKLFPDRCYDVGIAEQHAVTSAAGLALGGLHPVVAL
                     YSTFLNRAFDQLLMDVALLDAPVTITLDRAGVTGDDGASHNGMWDLSVLGIVPGIRVA
                     APRDAGTLREELAEAIEVDDGPTAVRFPKGAVIESVPAVDRVEGVDVLVRPGPADGHD
                     VLLVGVGAFAVLAMAAAQRLADQGIGVTVVDPRWVLPVPGVLMRMAAQHRLVVSLEDG
                     GRHGGFGWALAAALRDAELDVPLRDLGIPQRFLEHGSREEVLADLGLTAQDVARAVTG
                     WAAGLIGSLSEPTAVKAPRAR"
     gene            complement(3353..>3706)
                     /locus_tag="VHH53_15815"
     CDS             complement(3353..>3706)
                     /locus_tag="VHH53_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX2291613.1"
                     /translation="VTVVEASRRAVADGAAALADLTQVHFVTSRVERALDHLPGRPDV
                     VVLDPPRRGAGRQVAAAVADRGPTRIVQVACDPAALARDIAAYLQCGYRLLKVRALDA
                     FPMTHHVECVAVLAR"
BASE COUNT          611 a         1373 c         1204 g          518 t
ORIGIN      
        1 caccagcagc ccgtccacca actgctgggc gcgcagcgtt gcatcccgga tcaccgtcgc
       61 catgcggcgc agttcggcct cgtctgcggc gggatccgcc agcgtgacat ccacctcggt
      121 gcgcaggacc gccagcgggg tgcgcagttc atggctggcg ttcgcgacga accggcgttg
      181 ggcctcgaag gcggcttgca gccggtcgag catcgcgtcg aaggtgtcgg cgagttcggc
      241 cacctcgtct cgcggcccgg gcagtcgcag ccgctcatcc agcgattcgg cggacaaccg
      301 gcgcgcggtt gcggtcacgt cgtgcagtgg ccgcagcacc tgaccggtga tcacccaggc
      361 cagcagcgcg gtggcggtca ccgcgagcag gaaggccacc gacccgacct ccagcacgcg
      421 ttgcctggcc gcctcctgca gtgccgcccc gagctggtcg gcggccacgg gcacgccgtc
      481 gacctcgacg gtgctgcctg gcggtaggtc cggtaccgcg gctaccacac cgccgaccag
      541 caaccagccc aaccacaaca gcagcgcgct caccgccgcc gagagcccgg tggcaagcag
      601 ggtgagccga gcacgcagcc ccggcccgcg gcggggtctt accacccgct cagccgggcg
      661 cagagtcggt ggtgggtact cggtagccgg agccgaccac ggtctcgatg atgcccggct
      721 cgccgagctt tttccgcaac gtcatcatgg tcacccggac tgtggtcgtg aacgggtcag
      781 cgtgctcatc ccacacccgc tccagcagct cctcgctgct gaccacccct cccccggccg
      841 aaagcagcac ctcgagcacc ccgaactcct tacgagtgag atcaatcggg atgccggacc
      901 ggctgacggt gcgccgggac gggtccagca gcacgtcggc ggcgctgagt accggcggca
      961 ccgcgggggt ggcccggcga cccagcgcac gcagccgagc caccaactcg gcaaatgcga
     1021 acggtttggc catgtagtca tcggcaccca gggacagccc ggccacccgg tcagacaccg
     1081 tgccgctggc ggtgagcatc aatacccggg tcagggcacc ggatccggtg atctcggcgc
     1141 acagctggtc acccgacatc ccgggcaggt cgcggtccag gaccacgacg tcataccggg
     1201 tcaccgcagc cttctcatgg ccgctgtcac catcgtaggc gacgtccaca gccatgcctt
     1261 cacgccgcaa gccacgagcg accgcgtcgg ccagtggctg ctcgtcttcc acgatcagaa
     1321 tgcgcacccc tccacggtgc cacaccccct gacgccgcgg cgacggcgct ggctaccgcg
     1381 cccggggggc tttgaccgcg gtgggttcac tcagcgaccc gatcaatccg gccgcccagc
     1441 cggtcactgc tctggcgacg tcctgagcgg tcagcccgag atctgcgagc acctcctcgc
     1501 gggagccgtg ctcgaggaac cgctgcggaa taccgagatc acgaagcggc acgtcgagct
     1561 cggcatcgcg cagcgcggcg gccagcgccc acccgaaacc gccgtggcgc ccaccgtcct
     1621 ccaagctgac caccagccgg tgctgcgccg ccatccgcat cagtaccccg ggcaccggca
     1681 gcacccagcg ggggtcgacg acggtgaccc cgatcccctg atcggcgagc cgttgggcgg
     1741 cagccatcgc cagcaccgcg aatgcgccga ctcccaccag caggacgtca tgcccgtccg
     1801 ccggtcctgg gcggaccagc acatccactc cctcgacacg gtccacagcc ggcaccgact
     1861 cgatcactgc gcccttgggg aagcgcaccg cggtcggccc gtcgtccacc tcgatcgcct
     1921 cggccagctc ctctcgcaac gtgccggcgt cgcggggcgc ggccacccgg atccccggca
     1981 cgatgcccag taccgacagg tcccacatgc cgttgtggct ggcaccgtca tcgccggtga
     2041 caccggcccg gtccagggtg atggtgaccg gggcgtcaag caatgccacg tccatcagga
     2101 gctggtcgaa cgcgcggttg aggaatgtgg aatacagcgc caccaccggg tgcaacccac
     2161 ccagggccag ccccgccgct gacgtgaccg cgtgctgctc ggcgatgccg acgtcatagc
     2221 agcggtccgg gaaaagcttg gcgaaggcgg ccaggccggt cggacccggc atcgctgcgg
     2281 tgatcgcgac caggtcctcg cgctcaccgg ccaatctgat caattcctcg gtgaacacgc
     2341 tggtccagga aacaccggtg ggcgcgatgg gcaccccggt ctccggatcc atcaccttca
     2401 cctgatgcat cagctcggcg gcatccttct ccgcgggcgg gtagccgtta cccttgcgag
     2461 tcaccgcatg cacgatcact ggtccaccga agtccttggc tcggcgcaag gcggactcca
     2521 tggcctgcat gtcatgcccg tcgaccggac ccaggtactt caggcccagg tcctcgaaga
     2581 ggatctgggg cgtcagggcg tccttgagac cccgcttggc cgcgtgcaga ccggcataga
     2641 tcgcccggcc caccaccgga gtgcgttgca gggcctgttt accggtgcgc agcacccgct
     2701 cgtaaccggg ctgcagccgc agcgtcgaca ggtgatcggc cagccccccg atggtcggtg
     2761 agtaggagcg gccgttgtcg ttgaccacga tgaccaccgg ccggtcctgg ccggcggcga
     2821 tgttgttcag cgcctcccag cacatcccgc cggtcagcgc gccgtcaccg acgaccacga
     2881 cgacgtgcct gctcgccgca gggctgcttg cgccgtgggc gaaggctttt gccagcccgt
     2941 cggcataaga caacgcggcc gacgcgtgac tggactcgac ccagtcgtgc tcgctctccg
     3001 cgcggcaggg gtagccggac aagccacccc gcttgcgcaa ccggtcgaaa ccctcgatcc
     3061 gaccagtgag gatcttgtgc acgtacgcct ggtgaccggt gtcgaagatc accgggtcat
     3121 gcggagagtc gaacactcga tgcaccgcga gggtgagttc gaccgcgccg aggttggagc
     3181 ccagatgccc tccggtgcgg gacaccttgt cgacgagaaa cgtgcggatc tcggaggcca
     3241 actgggtcag cgcttccgca tcgagagcct tgacgtcctg cgggctgcgc accgactgca
     3301 atagcgtcac cgattcccca atctacggac ggctcagtct acggagaagg cctcagcggg
     3361 ccagcacagc cacgcattcg acgtggtgag tcatcgggaa cgcgtccagt gcacgaacct
     3421 tcagcagccg gtaaccgcac tggagataag ccgcgatgtc gcgcgccagc gcggcggggt
     3481 cgcaggccac ctgcacgata cgggtaggcc cgcggtcggc caccgcggcg gccacctgcc
     3541 ggccggctcc gcgtcggggc gggtcaagca ctaccacgtc cggacgccca ggcaggtgat
     3601 ccagcgcccg ctccacccgg ctggtgacga aatgcacctg cgtgaggtcg gccagcgccg
     3661 ccgcgccgtc ggccactgcg cgccgagagg cctccacgac cgtcac
//
LOCUS       DATADN010001234         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_142885, whole genome shotgun sequence.
ACCESSION   DATADN010001234 DATADN010000000
VERSION     DATADN010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142885"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1250
                     /gene="glgA"
                     /locus_tag="VHH53_15820"
     CDS             81..1250
                     /gene="glgA"
                     /locus_tag="VHH53_15820"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003796502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX2291614.1"
                     /translation="MRIGLLTREYPPEVYGGAGVHVGHLVSELRQRCQVEVHCFGAPR
                     PQTPHLRVHNHTVAPGLAAANPALQTLSVDLAMADALDGVDLVHSHTWYANLAGHLAR
                     TLYGMPHVVTAHSLEPRRPWKAEQLGGGYRVSSWVERTTYEHADAVIAVSDGMRADVL
                     DCYPTVHPERVHVVRNGIDTNTYRPVEETDVIRRLGADPARPIVAFVGRITRQKGVGH
                     LLAAAQHIDAGAQLLLCAGAPDTSEIEIETRTAVAALSAARPGVIWVADMLSTADVRQ
                     VLSAATVFACPSVYEPLGIVNLEAMACGTAVVASAVGGIPEVVDDGVTGLLVPYDEHD
                     PERFQRDLAHRVNDLLANPDLVTAMGQAGRKRAVTEFSWSAVADRTVDLYHELLS"
     gene            complement(1348..>2092)
                     /locus_tag="VHH53_15825"
     CDS             complement(1348..>2092)
                     /locus_tag="VHH53_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2291615.1"
                     /translation="VKLGTMGEYGTPNIDIEEGWLTVEHNGRTDRMLYPKKPGSFYHL
                     TKVHDSHNIEFGCRIWGLRATDLNQGVVYGQQTEQTARDARLATRLDYDAVFGTVLNR
                     FVIQAVLGHPLTVYGAGGQTRGIIDIRDTVECIRLACENPADPGEFRVFNQITETMSV
                     ADIAKTVSVSFPGNVEIEYLGNPRVELDAHYYNVVHAGLMDLGLAPHLLSETLIDSLF
                     GIVKQHIARADLSAMRPTVDWRSTTNPPR"
BASE COUNT          349 a          660 c          681 g          402 t
ORIGIN      
        1 cattcattgc cagggcttca tgggcttcat tgcggggttg atcgctgggg ttcgacgtca
       61 ggacgctagc gtgacgcggc gtgcgtatcg gactcctgac ccgggaatat ccgcccgagg
      121 tatacggcgg cgccggggtc cacgttgggc acttggtgtc cgagttgcgc cagcggtgtc
      181 aggtcgaggt gcactgcttc ggcgcccccc gtccgcagac gccgcacctg cgggtgcaca
      241 accacactgt tgcgccagga ctcgctgctg ccaaccctgc gttgcaaacc ctgtcggtgg
      301 acctcgccat ggccgatgcg ctggatggcg tggacctggt ccactcgcat acctggtatg
      361 ccaacctggc aggtcacctg gcgcggacct tgtacggcat gccgcacgtg gtcacggcgc
      421 attccctgga accccgcagg ccatggaagg ccgagcagct cggcggtggc taccgagtgt
      481 cctcctgggt ggaacgtacg acctacgagc acgccgatgc ggtcatcgcg gtcagcgatg
      541 gcatgcgagc cgacgtgttg gactgctacc cgaccgtgca ccccgagcgc gtgcacgtcg
      601 tgcgcaacgg catcgatacc aacacctacc gcccggtcga ggagaccgac gtgattcgcc
      661 ggctcggtgc cgacccggcg cggccgatcg tcgccttcgt gggacggatc acccgacaga
      721 aaggggtcgg gcacctgctg gcggcagcgc agcacatcga tgcgggtgcc cagttgctgc
      781 tgtgtgcggg cgcgccagat acgtcagaga tcgaaatcga gacccgcacc gccgtggcgg
      841 cgctgtccgc agcccgcccc ggtgtcatct gggtggcgga catgctgtcc accgctgacg
      901 tacggcaggt gctgtccgcg gcaaccgtgt tcgcctgccc ctcggtttac gagcctttgg
      961 gaatcgtcaa tctcgaggcg atggcgtgcg gtaccgcggt ggtcgcctcg gccgtcggtg
     1021 ggatcccgga ggtcgtcgac gacggcgtca ccggtttgct ggtgccttac gacgagcacg
     1081 accccgagcg gtttcagcga gacctcgccc accgcgtcaa tgatttactg gccaatccgg
     1141 acctggtcac agcaatgggc caggccggac gcaagcgagc cgtcactgag ttttcctggt
     1201 cagcagttgc cgaccgcacc gttgaccttt accacgagct cctctcgtaa tccaccctta
     1261 ccgcgttctg gatgcagact gcattaatcc atgcctgttt acagcagtct gtatctagaa
     1321 cacgggggcg gaggcaggcg ccagagttta gcgcggtggg ttggtggtgg agcgccaatc
     1381 tacagtgggg cgcatggcgg ataggtcggc tcgggcgatg tgttgcttga cgatgccgaa
     1441 cagggaatcg atgagcgtct cggagagcag gtgcggcgcc aagcccagat ccatcaggcc
     1501 agcgtgtacc acattgtagt agtgtgcgtc gagttctacc ctgggattgc ccagatactc
     1561 gatctccacg ttcccgggaa agctgaccga cacggtcttg gcgatatcgg cgaccgacat
     1621 cgtctcggtg atctggttga atacccggaa ctcgccagga tcggccggat tctcacaagc
     1681 cagccggatg cactcgacag tgtcccgtat gtcgatgatt cccctggtct ggccgcctgc
     1741 cccgtagacg gtcagcgggt gcccgagcac cgcctgaatg acgaatcggt tgagcactgt
     1801 gccgaagacg gcatcgtagt cgagccgggt ggccagccgg gcgtcccgcg cggtctgttc
     1861 agtctgctgg ccgtagacca caccctggtt gagatcggtc gcccgcaatc cccaaatccg
     1921 gcaaccgaac tcgatgttgt ggctgtcatg caccttcgtg aggtggtaga aggagcccgg
     1981 cttcttagga tagagcatcc gatcggttct accgttgtgc tcgacggtca gccagccctc
     2041 ctcgatgtcg atgttcggcg tgccgtactc gcccatggtg ccgagcttga cc
//
LOCUS       DATADN010001235         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1353164, whole genome shotgun sequence.
ACCESSION   DATADN010001235 DATADN010000000
VERSION     DATADN010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353164"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..449
                     /locus_tag="VHH53_15830"
     CDS             210..449
                     /locus_tag="VHH53_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291616.1"
                     /translation="MAGPAGIGTESVYGSDVSTSEPPTFPDAIGRDYGLGDDGLALIR
                     SDGYIGLLADCTDLDVLRGCRQDVLHTQTASHGQR"
     gene            712..840
                     /locus_tag="VHH53_15835"
     CDS             712..840
                     /locus_tag="VHH53_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291617.1"
                     /translation="MTLQRQSAPRAVPRLAAGAVLPVSVLALVARAALEVADNGGR"
     gene            827..2482
                     /locus_tag="VHH53_15840"
     CDS             827..2482
                     /locus_tag="VHH53_15840"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEX2291618.1"
                     /translation="MADDEVFAQAKTYRVGDFILQVTTWSGVAALRVVHATEPDRALW
                     ESVPGRGFLAAGVAETSIEENTTPASGFTLTDVDLVRFERQVVDEVRVGEGTAILHGS
                     LSGEQRTVGYAMTFSEPVPDQLRFEVVLTDRAGTPPCNRVFLRYRSAPDEHFFGFGQQ
                     LTYFDQKGRLLPVLVQEHGVGRGLPVVTQAVELKYGARSAGAWYTTEVSVPHYLTTQL
                     RSLFLENTEYCVFDLRDPVSAEIELFAGGMSGRILHGGSPLELIESYTQYAGRMRPLP
                     DWVHEGAIVAVQGGTNRAMAKLQALVAADVPVAAFWIQDWVGQRSTPAGSQLWWDWQL
                     DQTHYPNWEQLLDALRDQRNARMLIYINPFLADQPGHDQLYREALLNGYLVTGADGQP
                     ILIPNAGFSAGLIDLSNDDARTWIKDIIKDRLIDQAGASGWMADFGEALPFNAVLASG
                     ESADVWHNRYPEEWARVNRQAIEEAGQGSDAVFFSRSGYTRSPGISTLFWVGDQLQTW
                     DEHDGMKSAITGSLSGGVSGFGLVHSEPFPVVEAGRSGTRRGW"
     gene            2534..>2669
                     /locus_tag="VHH53_15845"
     CDS             2534..>2669
                     /locus_tag="VHH53_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX2291619.1"
                     /translation="MSSRTEVVGVSDPVSCTAVLPVGESTVLFVSDLLAAERRRRGTR
                     G"
BASE COUNT          421 a          898 c          901 g          449 t
ORIGIN      
        1 cgttgatcct gatcgccaac ttcgggcccc ccatcgtcat gctgatcacc ctgctctcga
       61 ccaacatcct ctgaacgagg ggacctctac gtcgacaccc gagccaacca gaggttcggc
      121 ggttcatgtg cgtcctgaca ccggcgccgt agtcagcatc aaagagttga cgtccatcga
      181 gtcagtcgct gtcagccgtg accgcgacaa tggcgggccc ggcaggcatc ggcacagaat
      241 ccgtgtacgg atccgacgta tccaccagcg agccaccgac ctttccggac gccatcggtc
      301 gtgactacgg tctgggtgac gacggtctcg cgctcatccg ctccgacggt tacatcggcc
      361 tgctcgccga ctgcaccgac ctggatgtcc tgcgcggctg tcgtcaggac gttctccaca
      421 cccagacggc ttcccatggt caacgctgac accgtggctc cacaatccga cctaggacgg
      481 ccgttcaccc gtatcttgac cgcggtcgcc ggagagcggc cgaacggagt gcggcgttcc
      541 cggccgcccg cccttgccgc ggtgcacgaa gcgcaaggcg ccgttcgtga aacgcgacgg
      601 acccccttct gagaaaggac ctgtggtttc ggtgtccctg caaacccggc ggtcggagct
      661 gcggtgtgcg accttgcagt cggcagccgt gcccggcgtg cggagcgggt cgtgacgctg
      721 cagcggcagt cggcgccgag ggctgtgccc cggctggcag ccggtgcggt gctgccggtc
      781 agcgtgctgg cgctcgtcgc ccgtgccgca cttgaggtgg cggacaatgg cggacgatga
      841 ggtgttcgca caagcgaaga cctaccgggt gggggacttc atcctgcagg tcacgacctg
      901 gtccggtgtc gctgcgctgc gtgtcgtcca cgccaccgaa cctgatcgcg cactttggga
      961 gagcgttccc ggccgggggt tcctcgccgc cggcgtggcc gagacgagca tcgaggagaa
     1021 caccactccg gccagtggct tcactctcac cgacgttgat ctggtccggt tcgagcgcca
     1081 ggtcgtcgat gaggtgcgcg tcggcgaggg gaccgccatc ctccatggga gcctttcggg
     1141 tgagcagcgg accgtcggct acgccatgac cttctccgag ccggtgccgg accagctgcg
     1201 gttcgaggtc gtactgaccg accgcgcggg gacgccccct tgcaatcggg tgttcctgcg
     1261 ctaccggtcg gctccggatg agcacttctt cgggttcggt cagcaactga cctacttcga
     1321 ccagaagggc cggctgctgc cggtcctggt gcaggagcac ggcgtcgggc gtgggctgcc
     1381 ggtggtcacc caggccgtcg agctgaagta cggcgcgcgc agcgcgggtg cctggtacac
     1441 caccgaggtc tcggtcccgc actatctgac cacccagctg cggtcgctgt tcctggagaa
     1501 caccgagtac tgcgtgttcg acctgcggga ccccgtcagc gccgagatcg agctgttcgc
     1561 cggcggcatg agtggccgca tcctgcacgg cggcagcccg ttggagctga tcgagtccta
     1621 cacgcagtac gccgggcgga tgcggccctt accggattgg gtgcacgagg gcgcgatcgt
     1681 cgccgtgcag ggcggcacca accgcgccat ggccaagctg caggcgctgg tggccgccga
     1741 tgtcccggtc gccgcgttct ggattcagga ctgggtcggc caacgcagca ccccggccgg
     1801 ctcccagctg tggtgggact ggcagctcga ccagacccac tatcccaact gggaacagct
     1861 gctcgacgcg ctccgcgacc agcgcaacgc ccgcatgctc atctacatca acccgttcct
     1921 cgcggatcag cccggccacg accagctcta ccgcgaggcc ctactcaacg ggtatctggt
     1981 gaccggcgcc gacggccagc cgatcctgat tccgaacgcc ggtttctccg cgggcctgat
     2041 cgacctgagc aacgacgatg cccggacctg gatcaaggac atcatcaagg accgcctgat
     2101 cgaccaggcg ggcgcgtccg ggtggatggc ggatttcggc gaggcgttgc cgttcaacgc
     2161 cgtactcgcc tccggggaga gcgcggatgt gtggcacaac cgctatccgg aggagtgggc
     2221 gcgggtgaac cgccaggcca tcgaggaggc cggccagggc agcgacgcgg tgttcttcag
     2281 ccgttccggc tacacccgca gcccagggat ctccaccctg ttctgggtcg gtgaccagct
     2341 tcagacctgg gacgagcacg acgggatgaa gagcgccatc accggatcgc tgtccggtgg
     2401 ggtctccggc ttcggcctcg tgcacagtga gccattccca gtagtcgagg caggcaggtc
     2461 agggacacgc cgagggtggt gagatggccg gaagggggcg caggacctcg gtaggaggga
     2521 cgtccttcca cagatgtcca gccgcaccga ggtcgtggga gtgtctgatc ctgtcagttg
     2581 caccgcggtg ctcccggtcg gggagtccac cgtgttgttc gtgtccgacc tactggctgc
     2641 cgagcgacgg cgacgcggca cccgaggcc
//
LOCUS       DATADN010001236         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_901622, whole genome shotgun sequence.
ACCESSION   DATADN010001236 DATADN010000000
VERSION     DATADN010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901622"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VHH53_15850"
     CDS             <1..242
                     /locus_tag="VHH53_15850"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEX2291620.1"
                     /translation="GAQQLYQWQTNWVLYRATAEALKHEKYLYLAAAGPYSTDDRQRV
                     LAERLEGLVSQEHAKWTESRQKDSHTSSSAEPKHS"
     gene            complement(327..596)
                     /locus_tag="VHH53_15855"
     CDS             complement(327..596)
                     /locus_tag="VHH53_15855"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX2291621.1"
                     /translation="MLVELVSNAAYAYPGWGHGPGAGGPGWWLIFPILFWVLVLSAIG
                     YVIYRRAPKQQARSSAERTLAERYARGEISEDELRQRRNVIRTKS"
     gene            926..>1610
                     /locus_tag="VHH53_15860"
     CDS             926..>1610
                     /locus_tag="VHH53_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005168059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HEX2291622.1"
                     /translation="MAIQGVECDRDHPPWAYTVGLTEYGVPELVATGLRLPRAAELLN
                     GVAAHSLHATADVRLEHPGAAGRVTDDRGHWRGTAAFGAGAVASRCSGRGLHRECASG
                     ADRRGARARDVKTGPATVPGRPATGYETTRGGCVRVVTTSLNQRIADELGVPERQVGA
                     AVGLLDGGATVPFVARYRKEATGGLDDAQLRTLEERLGYLRELEERRAAVLNSIREQG
                     KLNEALEAQI"
BASE COUNT          274 a          536 c          534 g          266 t
ORIGIN      
        1 agggcgccca gcagctgtac cagtggcaga ccaactgggt gctctaccgt gccaccgccg
       61 aagccctcaa gcacgagaag tacctctacc tcgccgctgc cggcccctac agcacagacg
      121 accggcagcg tgttctcgcc gaacgtctcg aaggcctggt ttcccaggag catgccaaat
      181 ggacggaatc acggcaaaaa gacagccaca cttcttcttc ggctgagcca aagcacagct
      241 aatcgtcgcc cgcatctgtc gctatgcctg taccgcgaat ttgcgcgcgg tacaggcatg
      301 gcttgcagca cgtcgaccga accgggtcac gacttggtcc ggatcacgtt cctccgctgt
      361 cgcagctcat cctcgctgat ctcgccgcgg gcgtaccgct ccgcgagagt ccgctcggcg
      421 gagcttcgtg cctgctgctt cggtgccctg cggtaaatca cgtacccgat tgccgacagc
      481 accagaaccc agaacagaat cgggaaaatg agccaccatc cgggcccgcc tgctccgggg
      541 ccatgtcccc agcccgggta tgcgtatgcc gcattgctca ccagttctac taacatcacg
      601 atctccctgt tgcttttggc cgggggtttg gtccctgacc gtgtacctcg atgttccgcc
      661 gccggtaccg ggatcgtcgt cggcttggca gaggcaattg gagtacgccg gggagtgcag
      721 tcaccgtgat cggcgtaccc cagcggaggt aggccgtatc ggctggtgat gcaccagcct
      781 gcgccatcga tcaggattgt cggtgcccgg gtgcatggtg gccagcgtcg ggatctgacc
      841 aggaggagac cacgtgcctg atatgcgacg gagcaacgta cgaggaatcc ctcgcccaca
      901 tggcgtggat cgtcgccgag cagggatggc gatccaaggc gtcgagtgcg atcgagatca
      961 cccgccatgg gcgtacaccg tcggcctcac cgaatatggc gtcccggagt tggtggctac
     1021 cggactgcgc ctgccgcggg cggccgagct gctgaacggc gtcgccgcgc actcgttgca
     1081 cgccactgca gatgtacggc ttgaacatcc gggcgctgca ggtcgtgtga ccgacgaccg
     1141 tggccactgg cgtggcacgg cggctttcgg ggcgggcgcg gtggccagcc ggtgctcggg
     1201 ccgcgggctg catcgtgagt gtgcgagcgg cgccgaccgg cgcggtgccc gggcgcggga
     1261 tgtgaagacc ggcccggcta ccgtgcctgg gcggccggct accggctacg agaccacccg
     1321 aggaggctgc gtgcgcgtcg tcaccacgtc gctgaaccag cggatcgccg atgagctggg
     1381 cgttcccgaa cgccaggtgg gtgctgcagt cggcctgctc gacggcggcg ccaccgtgcc
     1441 gttcgtcgcc cgctaccgca aggaggcgac cggcggtctc gacgacgcac agctgcgcac
     1501 acttgaggaa cggctcggct acctgcgtga actggaggag cgccgggcag ccgtcctcaa
     1561 ctcgatccgc gagcagggca agctcaacga ggccctggaa gcgcagatcc
//
LOCUS       DATADN010001237         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_300197, whole genome shotgun sequence.
ACCESSION   DATADN010001237 DATADN010000000
VERSION     DATADN010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300197"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VHH53_15865"
     CDS             <1..352
                     /locus_tag="VHH53_15865"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2291623.1"
                     /translation="GPPRMTATTGAGALAHGPGMGNGAAREKMEDLSTSTNAPAPPAS
                     APTPNVPPAAHVTTNEGPLSLEAMERNHIIAVLRQTNGVVEGPNGAARLLNLKPSTAR
                     FRMKKLGIRKIEYL"
     gene            complement(583..1857)
                     /locus_tag="VHH53_15870"
                     /pseudo
     CDS             complement(583..1857)
                     /locus_tag="VHH53_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          397 a          679 c          575 g          338 t
ORIGIN      
        1 agggccaccg cggatgacgg ctaccaccgg tgccggcgcg ctcgcccacg gacccggtat
       61 gggcaatggc gcagctcgcg aaaagatgga agatctgtct acgagcacga acgctcccgc
      121 gccacccgcc agcgcgccga ctccaaacgt gccgccagcc gcacacgtca cgacgaatga
      181 aggcccgctc tccctcgaag cgatggagcg caatcacatc atcgccgttc ttcggcagac
      241 caacggcgtg gtcgagggcc cgaatggcgc cgcacgcctg ctgaacctga aacccagcac
      301 cgcacgtttc cgcatgaaga aactcggtat ccgaaaaatc gagtacctct gacccgcccc
      361 cgctttgatc gcagccagcc aatcgccgag cgctgcaccg gacgccgctc gcttatctgc
      421 cgctgtacct acaccagcac tacaccctgc ccctcccacg cccgctgaat cccggtgcca
      481 cgacgtcaaa gcgttgccgc gatcaaagag tgacttgcgg gggcttcatc ggcagggaca
      541 gccgatgcag agcccccatg gactaatggc acttagttaa actcatgcca cacgcagttg
      601 tgcctgtaac tgcctgcgcg ccagcgcccg aggcacttgc agtcggggat aggttttggg
      661 tcgtcgcttg cgggcgcggg gctctattct gcccggccgg ctgcccacgc tgagttgggc
      721 gaggagccgg aagaaatcag cacgatgcac accgacccac tcgatccaca actgcacgct
      781 gtgtttgaag ctcaaggttc gcggatgcac gccggcctgt tgcgcggcct gcgccatcaa
      841 caggcggatc aggttatagg cgagcaggta cacccacagc tccttatgga ccatctgcgg
      901 gcttttgcag cttaagacct gcattcccaa ggtggtcttg atgttgcgga tgtccagctc
      961 aatgtgccag cggcgtgcgt acagctgtga tagctcgttt ttgcgcacca ctcgcgcatc
     1021 gtgcatcgtg gtcaccaaaa tctgcccatt cacccgcact tcccgaaccg tcagctcact
     1081 gggaagcgct gcgtactgct gagccgtcat ccattggggt tgtcggggtt tgggccaatg
     1141 caccagatga tcgcgctcgc ccaaggagcg gccgcgacga aaatcggtgc tgcgcgagcc
     1201 gtgctgctcg aacagcacat ccaccccaca cgcctgcaac gccgcgatca caaagtagtt
     1261 gcaatacaac gcatcgccca gcaatacatc tccagggcgt aacacctgca gctggcggcg
     1321 aaacagctcc tgctcactgc atcccttgcc cgctttggcc ccaaaggcgg cgctgagcaa
     1381 cgcgccgctg gacaaacaga tgatccccac cagcagaccc aggggaaaac ccgccccttc
     1441 cgcctggccc aggtgctgcg gatagcactc ctggttcaca gccgtatccg gcatggagaa
     1501 gaccgtcccg tcgaccagca tcaccgcccg atcgcgccag cgccaggcgc acgcggcccg
     1561 atccgacagc agacgaccac tgtggcaggc cagctgcctg atcagctcca gcggtaaccg
     1621 ttggcgtgcc tggcaatagg ctcccgtgcg gatgctgtgc acgtgcaagc cttcggccgc
     1681 gcgttgcgct gcccaggcat tgacggcccg ctggcaggaa cgatccgatt gcagcacctg
     1741 ctgcaggaac atcgacaagg tcaccgtcgg cggatacagc cgttcgcgat gctcgggcag
     1801 gtgagcctcc gtcacctgca gcagctcagg cccggtgagg atgttgaaga actccagccc
     1861 acccgtttca cccacctgcc gatgaatccg atcgcgatgt tgctgccgct ggaaacgcac
     1921 attacgatcg atcaaggctg gcctcggagt ggctgggttg gtgtgattgc ggtaggggca
     1981 tccggcaac
//
LOCUS       DATADN010001238         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_901904, whole genome shotgun sequence.
ACCESSION   DATADN010001238 DATADN010000000
VERSION     DATADN010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901904"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1988
                     /locus_tag="VHH53_15875"
     CDS             309..1988
                     /locus_tag="VHH53_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEX2291624.1"
                     /translation="MSPSPEVGPLIVQSDKTLLLEVDHPAAGMARAAIAPFAELERAP
                     EHVHTYRVTPLALWNARAAGHDAEQVVDALVRFSRYAVPQPLLVDVVDTMGRYGRLQL
                     VHHPAHGLVLASLDRAVLEEVLRHRKIAPMLGARVDDDTVAVHPSERGRLKQALLKAG
                     WPAEDLAGYVDGEAHPIELAEDGWSLRDYQRDAVTGFWAGGSGVVVLPCGAGKTLVGI
                     GAMAQAQATTLILVTNTVAGRQWKRELVARTSLSEDEVGEYSGERKEIRPVTIATYQI
                     MTRKSKGQYRHLDLFDSRDWGLIIYDEVHLLPAPVFRMTADLQSRRRLGLTATLVRED
                     GQEGDVFSLIGPKRYDAPWRDIESQGWIAPADCVEVRVTLTDAERLGYATAEADERYR
                     VCSTARSKLAVVRAIMDQHPDEPTLVIGAYLDQLEELGEALDAPVVQGSTRNSEREAL
                     YDAFRRGEVRTLVVSKVANFSIDLPEASVAVQVSGTFGSRQEEAQRLGRLLRPKGDQR
                     QAHFYSVVSRDTLDTDYAAHRQRFLAEQGYAYRIVDADDVLGPQQPPHGCL"
     gene            complement(2063..2947)
                     /locus_tag="VHH53_15880"
                     /pseudo
     CDS             complement(2063..2947)
                     /locus_tag="VHH53_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465852.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(3123..3941)
                     /gene="map"
                     /locus_tag="VHH53_15885"
     CDS             complement(3123..3941)
                     /gene="map"
                     /locus_tag="VHH53_15885"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2291625.1"
                     /translation="MTGGGVRAVVRRLRGRTSIELKSPGEIEAMRAAGAVVARTLRLL
                     EVHAKPGVSTGELDELAEQSIREAGAVPSFKGYHGFPGSICSSVNEQVVHGIPSREQV
                     LNDGDLLSIDCGAILDGWHGDAAVTVPVGTMADADVRLSAACRAALEAGIAAARSGGR
                     LTDISHAVETAALAAATAHGVPYGIVADYGGHGIGTAMHMDPFLPNVGRPGRGPRLVP
                     GMTLAIEPMLTAGGDKTEELDDGWTVVTLDGSRAAHWEHSIAVTDGEPLVLTAD"
     gene            complement(3950..4501)
                     /locus_tag="VHH53_15890"
     CDS             complement(3950..4501)
                     /locus_tag="VHH53_15890"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2291626.1"
                     /translation="MRILIVGPPGAGKGTQAAVLSAELGVPHISTGDLFRAHVDDGSE
                     LGQQVKQYLNSGALVPDELTNEMVTQRLAGSDCRAGFLLDGFPRTVSQARMFADLLRD
                     LDCEIDVVLELDAPEDLVVQRLLARGREDDTEDVIRRRQQLYREETAPLLDFYADRLI
                     TVPATGSVHDVTGRALDALRTRA"
     gene            complement(4501..>4987)
                     /locus_tag="VHH53_15895"
     CDS             complement(4501..>4987)
                     /locus_tag="VHH53_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2291627.1"
                     /translation="SSLLYLPYLIARLTTSPTGEPGWWQNFISRYLVDQSSWVHISLY
                     FLMIIFFTYFYVAITFNPDERAEEMKKFGGFIPGIRPGRPTAEYMGFVLNRITLPGSL
                     YLGTVAVLPNLFLDLTGSGQNQNFPFGGTAVLIMVGVGLDTVKQIESQLMQRNYEGFL
                     R"
BASE COUNT          847 a         1704 c         1640 g          796 t
ORIGIN      
        1 cacggacgat acgacggcct gggcagggcc ttcccgcggg tctcgtagaa cctcctctct
       61 gctcgccgag ggcgccagtg ttctgagctc acaacacccg ctcggcgtag acgacttgac
      121 caggatggtc gtactcccct gctctgagtg atatgtcgag ccgccagtcg aggagggctc
      181 actctggagc ggcagacagc ggcggacgcc accccggtcc tgcgtcagcg gtgccgccgc
      241 acctgcgaca ctggactcgg ccgagtcgac catcagccgc ggtgctaatc aatccggagg
      301 aacacacgtt gagcccatcc cctgaggtcg ggccactcat cgtccagtct gacaagacgc
      361 tgctgctaga agtcgatcac cctgcggccg gcatggcgcg cgcggcgatc gcgcccttcg
      421 ccgaactcga gcgcgcaccc gaacacgtgc acacctaccg ggtcaccccg ttggcgctgt
      481 ggaatgcgcg cgccgcggga cacgatgctg agcaggtcgt tgacgcgctg gtgcgctttt
      541 cccggtacgc cgtgccgcaa ccgctgctgg tcgatgtcgt ggacacgatg ggccgctacg
      601 gccggttaca gctggtgcac cacccggcgc acgggctggt gctggcatcg ctggaccgcg
      661 ccgtgctcga ggaggtactc cggcaccgca agatcgcccc catgctcggg gcgcgggtgg
      721 atgacgacac cgtggcagtg catccctccg agcgcggcag gctcaagcaa gcgttgctca
      781 aggccggttg gccggctgag gatctggccg gctacgtcga cggcgaggcg cacccgatcg
      841 agctggccga ggacgggtgg tcgctgcgcg actaccagcg cgacgcggtg accggattct
      901 gggccggcgg ctccggagtc gtggtactgc cctgcggcgc cggcaagacc ctggtgggta
      961 tcggtgcgat ggcacaagcc caagcgacca cgctgatcct ggtcacgaat accgttgccg
     1021 gacggcagtg gaagcgcgag ctggtcgccc gcacgtcgct gtctgaggac gaggtgggcg
     1081 agtactccgg cgaacgcaag gagatccgcc cggtgacgat cgcgacctac cagatcatga
     1141 cccgcaagtc gaagggccag taccggcacc tcgatctctt cgactcccgg gactggggat
     1201 tgatcatcta cgacgaggtg cacttgttgc ccgccccggt cttccggatg accgccgact
     1261 tgcagtcccg tcgccggctc gggctgaccg ccaccctggt gcgcgaggac ggccaggaag
     1321 gtgacgtgtt ctccctgatc gggcccaaac gctacgacgc gccatggcgt gacatcgagt
     1381 cccagggctg gatcgcgccg gcggactgtg tcgaggtgcg ggtcaccctg accgacgccg
     1441 agcgtttggg ctacgccacc gccgaggccg acgagcgcta ccgggtgtgc tccaccgcgc
     1501 gaagcaagct ggccgtcgtc cgcgcgatca tggatcagca ccccgacgag ccgaccctgg
     1561 tcatcggcgc gtacctggat caactggagg aactcggcga ggcgctggac gcaccggtgg
     1621 tccagggctc gacccgcaac tccgagcggg aggccctcta cgacgcgttc cgccgcggcg
     1681 aggtgcgcac cctggtcgtg tccaaagtgg ccaacttctc catcgacctg ccggaggcgt
     1741 cggtggccgt gcaggtctcc ggcacgttcg gctcccggca ggaggaggca cagcggctgg
     1801 gccgattgct gcgacccaag ggcgaccaac ggcaggcgca cttctactcg gtggtgtccc
     1861 gggacaccct ggacaccgac tacgccgcgc accgccagcg cttcctcgcc gaacagggct
     1921 atgcctaccg gatcgtcgac gccgacgacg tcctcggccc ccagcagccg ccacacggat
     1981 gcttgtagcg ctcagaagag gcctgtgagc taccacaagc atccatgtgg cgctgacatc
     2041 gggtgtcgaa ctgcggacca gctcactcgg cgggagcgga gctggctaac cgcagccggg
     2101 gtgggagctg cttgtccaaa ccgaagggtt caaggttgtg gtccaggacg ccgtcgaacg
     2161 cggcagcgat gtcctcggtc gtccagcact cccgatgcag caccggctcc ttgatcccgg
     2221 gctggcgcac gatgtgcaac tgatcaccgg tgaatcggac cagctgaccg gtgattccct
     2281 cggcccggtc tgagagcagg taagtgatca gtggagctcc ccggtccggg gttcgacgcg
     2341 gtgtgacgcg caactgcgcc gcgggggaac gctccaccat ccgggtccag gccaccgggc
     2401 agatcccgtt gacccggata cccagctctg ccatgtccag ggcccaggag taggtcaggg
     2461 acgcgacagc gcccttggag gccgcgtagg taccggcggc acgttgcccg aacgacgagc
     2521 ccgatgagat gttgacgatc gacccggcgc gttgtgtgcg catctcccgt gctgccgcga
     2581 tgccgcagta gaggacgcct agcacgttga cctcgatgag ttcgcgggcg acgtcaacat
     2641 cgtcgtccca gggcagcgcc gcgtagttga gcgcggcgtt gttcaccagg ccgtcgacct
     2701 tgccgaactc ggccaggcac cgctccacaa ccccactggc ctggtccgga tcgcgtaccg
     2761 attgggcgct ggccaccgcc cgcccgccgc agcggcggat tcgacctgcg accgcctctg
     2821 ccagttcgcc gtcgatgtcg ttgaccacga ccgaggctcc agaccgggcg gcatccaatg
     2881 cataggcctc tcctagcccg cggcccgctc aggtgatcac gatcgccttg ccttcgagga
     2941 tgcccatggc tgcccgcctt cggagaggac cggtgcctga gggcccggcg atgacgccag
     3001 tctccggtgg aaagggaccg ggatggcccg cagctcagcg aagggtcact gcccagcgcc
     3061 aagcgggtgg ggccgttcgg cgcaacgggt agccgccgac tagtcgcttc ggtaccccac
     3121 gactagtcgg cggtgagcac caacggctcg ccgtcggtca cggcgatgga gtgctcccag
     3181 tgggcggcac gggacccgtc cagggttacc acggtccacc cgtcatcgag ttcctcggtc
     3241 ttgtcgccgc ctgcggtgag catcggttcg atcgccaggg tcatcccagg taccaggcgg
     3301 ggaccgcggc ccggacggcc cacattgggc agaaatgggt ccatgtgcat ggccgtgccg
     3361 atgccgtgac cgccatagtc ggccacgatc ccgtagggca ccccgtgtgc ggtggcggcg
     3421 gccagcgccg cggtctccac cgcatgcgaa atgtcggtca gcctgccgcc cgatcgtgcc
     3481 gccgcgattc cagcctccaa cgcggcccgg caggctgcgg acagccggac gtcggcgtcc
     3541 gccatggttc cgacaggcac tgtgaccgcg gcgtcgccgt gccagccgtc gaggatcgcg
     3601 ccacagtcga tggatagcag gtcgccatcg ttgagcacct gctcacgact cggtatccca
     3661 tgcaccactt gctcgttcac cgagctgcag atcgatccgg ggaatccgtg atagcccttg
     3721 aaggacggta cggccccggc ctcccggatc gactgttcag cgagctcgtc gagctcaccg
     3781 gtactcacgc cgggcttggc gtgaacctca agtagccgca gggtccgcgc aaccaccgca
     3841 ccggcggcac gcattgcctc gatctcgcca ggagatttga gttcgatgct ggtccgtccc
     3901 cgcaagcgtc gcaccacagc acgcacccca cctccggtca tcccgcttgt cacgctcgag
     3961 ttcgcaacgc gtccagtgcc cggccggtca catcatgcac cgaaccagtc gccggcacgg
     4021 tgatcaaccg atcagcgtag aagtcgagca gcggcgcggt ctcctcgcgg tacagctgct
     4081 ggcgacgccg gatcacgtcc tcggtgtcgt cctcacgccc gcgggccagc aggcgctgga
     4141 ccaccagatc ctcaggcgca tcgagctcga gcaccacgtc gatctcacaa tcaaggtcgc
     4201 gcagtaggtc cgcgaacatc cgcgcctggc tgaccgtgcg gggaaatccg tcaagcagga
     4261 accctgcccg gcagtcactg ccggcaagcc gttgcgtgac catctcgttg gtgagctcgt
     4321 cgggcaccag cgcaccggag ttcagatact gcttgacctg ctggcccagc tcactcccat
     4381 cgtcgacatg agcccggaac aggtcaccgg tcgaaatgtg tgggacgccg agctccgcgc
     4441 tcagcacggc cgcctgggtg cctttgcccg cgcccggcgg gccaacgatg aggattcgca
     4501 ctaccgcagg aacccttcat agttgcgctg catcagctgg ctctcgatct gcttcacggt
     4561 atccagtccg acccctacca tgatcagcac agcggtgcca ccgaagggga agttctggtt
     4621 ctgcccacta ccggtcaggt cgaggaacaa gttgggcagt accgccacgg tacccagata
     4681 cagcgagcca ggcagggtga tgcggttcag cacgaagccc atgtattcgg cggtcgggcg
     4741 ccccggccgg atgccgggga tgaaaccgcc gaacttcttc atctcctcgg cgcgctcgtc
     4801 ggggttgaag gtaatcgcga cgtagaaata cgtgaagaat atgatcatca agaagtacag
     4861 cgagatgtgc acccagctgg actggtccac caggtagcgg ctgatgaagt tctgccacca
     4921 tcccggctcg ccggtgggcg aagtggtcag ccgcgcgatg aggtagggca ggtagagcag
     4981 tgacgac
//
LOCUS       DATADN010001239         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_300392, whole genome shotgun sequence.
ACCESSION   DATADN010001239 DATADN010000000
VERSION     DATADN010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300392"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VHH53_15900"
     CDS             <1..259
                     /locus_tag="VHH53_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018681973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX2291628.1"
                     /translation="QMALPEHGFELFAEHGHRLPQLTAARLPEGVVDAKARAALREDF
                     GIEVGGGLGPLTGAGWRIGLMGHNAQDRAVTTLLAALAAWS"
     gene            complement(308..1138)
                     /locus_tag="VHH53_15905"
     CDS             complement(308..1138)
                     /locus_tag="VHH53_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2291629.1"
                     /translation="MSDVVTLNNGVRMPQLGFGVFQVPVEDTEAVVSAALEVGYRSID
                     TAAAYQNEEGVGRAIASSGLARDAVFVTTKLWNAHHGYDRALRAFDASAQRLGLETLD
                     LYLIHWPVPAANRYVETWKALEKLASDGRARAIGVSNFGVEHLRRLVAETGTVPAVNQ
                     VELHPYFQQTDLRAEHAEHGIATEAWSPLGQGGALLSDATITRLADRHGVTPAQIVLR
                     WHLQLGNIVIPKSANPARMRQNLDVFGFELSAEDLSAIADLETGKRIGPDPATANFGT
                     "
     gene            complement(1250..>1829)
                     /locus_tag="VHH53_15910"
     CDS             complement(1250..>1829)
                     /locus_tag="VHH53_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX2291630.1"
                     /translation="LWTRNIYKPYLNRNADSRSEAQQAKLASLVVKLGAVGFVVFIDP
                     QYAIDLQLIGGVIILQTLPAVALALYTRWFHRWGLLAGWAAGLAWGLSLLYGIPNPTT
                     GAAHFGGSALRLGALSILGWEPFAGSSIQIYAGLIALIGNLVVAALVTIVLRRLRVFN
                     GTDETDPQDYHADEGSPRLKPVAGILQQTTST"
BASE COUNT          323 a          589 c          611 g          306 t
ORIGIN      
        1 gcagatggca ctgcccgagc acggtttcga attgttcgcc gagcacgggc accggttacc
       61 gcagctcact gcggcccggc tacccgaggg ggtggtggac gccaaggccc gtgccgcgtt
      121 gcgggaggac ttcggtatcg aggtcggtgg cggtctagga ccgctgaccg gcgccggttg
      181 gcggatcggc ctgatgggcc acaacgctca agaccgcgca gtgaccacgc tgttagctgc
      241 gctagcagcg tggtcgtgag tgggaaacag tgttgtaaca ctgtttccca ctcacgagct
      301 cacgagctca cgtgccgaag ttggcggtag cgggatctgg gccgatccgc ttgccggtct
      361 ccaggtccgc gatcgcagag agatcctcgg ccgagagctc gaagccgaag acgtcgaggt
      421 tctgccgcat ccgcgcggga ttcgccgact tggggatcac gatgtttccc agttgcaggt
      481 gccagcgcag cactatctgg gccggcgtca cgccgtggcg atcggcgagc cgggtgatgg
      541 tggcgtcgga gagcagtgcg ccgccctggc ccagcgggct ccacgcttca gtggcgatcc
      601 catgctcggc gtgctcggca cgcaggtcgg tctgctggaa gtacggatgt agctcgacct
      661 ggttgacggc aggcaccgtg ccggtctcgg cgaccaaccg ccgcaagtgc tcgacgccga
      721 agttggacac cccgatcgcc cgcgcccggc cgtcggatgc cagcttctcc agcgccttcc
      781 aggtctcgac gtaccggttc gcggcgggca ccggccagtg gatcaggtag agatccaagg
      841 tctccaggcc cagccgctgc gcgctggcgt cgaacgcgcg tagagctcgg tcgtagccgt
      901 ggtgagcgtt ccacagcttc gtggtcacga agaccgcgtc acgggccagc ccggaggacg
      961 cgatcgcccg tccgacgccc tcctcgttct ggtatgccgc cgcggtgtcg atgctgcggt
     1021 atccgacctc cagcgccgcg gacaccactg cctcggtgtc ctccaccggc acctgaaaca
     1081 ctccaaaacc aagctgcggc atccgcacac cgttattcaa cgtaacgaca tcactcatcc
     1141 ttacaacgta cccgaatcgc gttctggagg cagactgctg ttatcgggtg ccgaataaca
     1201 gcagtctgca tccagaatgc gcgaaagaac gcgaaaacga gcgcacttat taggtcgagg
     1261 tggtttgttg gagtatgccg gctactggtt tgaggcgcgg cgagccctcg tcggcgtggt
     1321 agtcctgcgg gtcggtctcg tcggtgccgt tgaacacccg cagccgccgg agcacgatgg
     1381 tgaccagcgc ggcgaccacg agattgccga tcagcgcgat gaggccggcg tagatctgga
     1441 tcgaagagcc ggcgaagggc tcccacccca gtatcgacag cgccccgagt cgtagtgccg
     1501 agccgccgaa atgcgccgca cccgtggtgg ggttggggat gccgtagagc agcgacaagc
     1561 cccacgccag cccagcagcc catcccgcga gcaggcccca gcggtgaaac caccgggtgt
     1621 agagggcaag tgccaccgca ggcagggtct gcaagatgat cactccgccg atcagctgca
     1681 ggtcgatcgc atactgcggg tcgatgaaga ccacgaaccc caccgcgccg agcttgacta
     1741 ccagcgaggc gagcttggcc tgctgcgcct cggaccggga gtccgcattc cggttcaggt
     1801 acggcttgta aatgttgcgg gtccacagg
//
LOCUS       DATADN010001240         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_300948, whole genome shotgun sequence.
ACCESSION   DATADN010001240 DATADN010000000
VERSION     DATADN010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300948"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VHH53_15915"
     CDS             complement(<1..1167)
                     /locus_tag="VHH53_15915"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2291631.1"
                     /translation="MPRLPVLLVLLGVVVGCSSQSTPISPSIPTQSPVTGGSAVPPST
                     PSAPEIRVDVVAAGLEYVWDIGFLPDGSALITERPARIRLLSSTRPGAQVTDVVAELG
                     DVYVAGEGGLMGLAVHPDFASTRRFTTCQTHQQGERPVDVRLVTWELSPDGARASRVA
                     DLLVAGLPLNPSGRHSGCRPTIGPDGYLYVGTGDSARAAVPQDRTQLGGKVLRIDLNT
                     GGPAPGNPFATSPNPAERLVFTYGHRNVQGVTVQPGTDRLFSAEHGPRVDDEINILRA
                     GKNYGWDPSSGGTEDSYDESVPMTDLQRFPDAVPAVWSSGGNIEAICGAAFMTGEQWG
                     PMQGLLAVAALRGSKLLLMRVDSDGKVTATGQLPQLDDSYGRLRAVRTGPDGALY"
     gene            complement(1222..1893)
                     /locus_tag="VHH53_15920"
     CDS             complement(1222..1893)
                     /locus_tag="VHH53_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2291632.1"
                     /translation="MTFLRVLLVDSHAMLTDALTVQLSTAQDLWVVGSCTPDDARLAD
                     IVHMLRPNVITTDIGPAAAVTGGMVRKLSAAWPAAHVVVLTGSHDAAHAVAAARAGAA
                     AWVSKESSLDHLLEVLRAVPLGHAYYPPRLLGVVLRELLDDARCARDHSARLDVLSYR
                     EREVLLRMVDGQPVSQIAVESVVSTSTVRTHVRSILTKLGVRSRLEAVSVARAAGLRP
                     TGDQA"
BASE COUNT          352 a          718 c          714 g          331 t
ORIGIN      
        1 atacagggcg ccgtcgggcc cggtgcgcac cgcgcgcagc cggccgtagc tgtcgtccag
       61 ctgcgggagc tgtccggttg cagtcacctt cccatcggag tccactcgca tcagcagcag
      121 cttggaaccc ctcagcgccg cgacggccag caggccctgc atggggcccc actgctcacc
      181 ggtcatgaat gcggcaccgc agatcgcttc gatgttgccg cctgagctcc acactgcggg
      241 cactgcgtcc gggaagcgct gcaagtcggt catcgggacg ctttcgtcgt agctgtcctc
      301 ggtacctccg ctagacggat cccagccgta gtttttccct gcccgcagga tgttgatctc
      361 gtcgtccacc ctcgggccat gctcggcgct gaaaagtcga tcggtgcccg gctgcaccgt
      421 cacaccctgg acattgcggt ggccataggt gaacacgagc cgttctgcag ggttcgggga
      481 agtggcgaat gggttgcccg gcgcgggacc gccggtgttg aggtcgatac gaagcacctt
      541 gccgccgagc tgagtgcggt cctgtggcac ggccgctcgt gcggagtctc cagtgccgac
      601 gtagaggtag ccgtccgggc cgatggtcgg gcggcatccg gagtgcctac cagaggggtt
      661 cagcggcaga ccggcgacca gcaggtcggc cacgcggcta gcccgggcac cgtcgggtga
      721 cagttcccag gtgaccagcc ggacatccac cggcctttcg ccctgctggt gagtctggca
      781 ggtggtgaac cggcgagtgc tcgcgaaatc gggatgcacc gccagcccca tcaggccgcc
      841 ctccccggcg acgtagacgt caccgagttc ggcaacgaca tcggtgacct gcgcgccagg
      901 cctggtcgag gacagcaacc ggatgcgtgc cgggcgctcg gtgatcagtg cagatccgtc
      961 gggaaggaat ccgatatccc atacgtactc cagccccgca gcgaccacat cgacccggat
     1021 ctcgggggcc gatggagtgc tcggtggcac cgccgagcca ccggtgaccg ggctctgggt
     1081 gggaatgctg ggactgatcg gggtgctctg gctcgaacaa ccgaccacaa caccgagcag
     1141 caccagcaac acaggcaggc gaggcatggg gtgatcctcc ccgatgtgga ggtaggccgg
     1201 tggtgaagct ggctgacccg gtcaggcttg atcaccggtg ggccgaagac ctgcggcgcg
     1261 agcgacgctc accgcctcga gacggctgcg cacccccagc ttggtcagaa tgcttcgcac
     1321 gtgggtccgg acggtgctgg tggataccac cgactcgacg gcgatctggc tcaccggctg
     1381 gccgtccacc atgcgcagca gcacctcgcg ctcgcggtag ctgagcacat cgagccgcgc
     1441 gctgtggtcg cgggcgcagc gcgcgtcgtc gagcagctct cgcagcacaa cgccgagcag
     1501 gcgcggtggg tagtaggcat gaccgagtgg tacggcgcgc agcacctcca ggaggtgatc
     1561 cagcgagctc tccttggata cccaggccgc cgcaccggcc cgcgccgctg cgacggcgtg
     1621 agccgcatcg tggctcccgg tcagcacgac cacatgtgct gcgggccagg cggcactgag
     1681 cttgcgcacc atcccgccgg tgacggccgc tgcagggcca atgtccgtgg tgatcacgtt
     1741 cgggcgcagc atgtggacga tgtcggccag gcgggcatcg tctggcgtgc agctaccgac
     1801 aacccacaga tcttgcgcgg tggacaactg gacggtcaag gcatcggtca gcatggcgtg
     1861 actatcgacg agcagcaccc gcaagaacgt cacgggtgca actgtgcccg gcagcgtccg
     1921 cgggcggcaa tacccgatct ccgggacacc atccggccca ggtttcaatc cgctgccctc
     1981 gccgaggaca tgctcacctg ctcgcccagt acctgctggc ccaatacctg ctggctcagc
     2041 atgggccggg cctccaccat gcggcgagcc ggactgaccc atctcccggt ggacttcagc
     2101 caggccagcg gcaat
//
LOCUS       DATADN010001241         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1354300, whole genome shotgun sequence.
ACCESSION   DATADN010001241 DATADN010000000
VERSION     DATADN010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354300"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..330)
                     /locus_tag="VHH53_15925"
     CDS             complement(115..330)
                     /locus_tag="VHH53_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291633.1"
                     /translation="MPWTNNPSEQALKGPKRHQAVSGYWHTLTTLADYCRGRSYLVSA
                     HNHGVRSIDAIHDALTGNPWLPTIATA"
     gene            complement(448..1614)
                     /locus_tag="VHH53_15930"
     CDS             complement(448..1614)
                     /locus_tag="VHH53_15930"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2291634.1"
                     /translation="MIIWVVSAPEPPEPSYEELAALVVGLVARVETLEAENAELRRRL
                     GMNSTNSSTPPSKDSIGAKAARRADRSSRERSKDRKPGGQPGHKGSGLAPAVTPDRTQ
                     TLPPPGACSGCGGDLTDAVDVGMSWAQVWDILPIMMEKVHYLLPRRRCGCGHTTTAAP
                     PFGTAGTVTYGPNINAAAILLASEGNVPLERTAMLMEALLGAPVSSGFVARALQRFAQ
                     RLAVAGFDDAMTTALRAQDVLCADETPTNVIHNNTDAHGTPVPGSPHAVTVRTPDARL
                     VWYAPIGSRSKNAIADLGVLAGYTGYLVRDDYAGWHQFDDQLDGVQQCAAHLIRHAKG
                     VLELHPTQQQWAGEIITVLREAATAVADAKADERDQLDPQLLADLRQRYDHAVA"
BASE COUNT          267 a          573 c          644 g          337 t
ORIGIN      
        1 taccactttg gatcccgttg ctggaagcag tagcgttgac tccggtccgc agcgagtcag
       61 cactcgtccc tactctgaca ccctgtagtg tccattcacg ggtgagctgt gaggtcacgc
      121 cgttgcgatg gtggggagcc aggggttgcc ggtaagggcg tcgtggatgg cgtcgatgga
      181 gcggacgccg tggttgtggg cgctgaccag gtaggagcgg ccgcggcagt agtcggcgag
      241 tgtggtgagt gtgtgccagt agccggagac ggcttgatgt cgtttggggc ctttgagggc
      301 ctgctcactg gggttgttgg tccagggcac ggcgaggttg cgggtgaagg tccagacttg
      361 atcggccttg tcgtggaggc gtttggcgag gttgtatccg gggtggttgc ccttggccca
      421 gttgcggtgc tggttggtgg tgattcccta ggcgactgcg tggtcgtagc gttggcgtag
      481 gtcggcgagt agctgtgggt cgagttggtc gcgctcgtcg gctttggcgt cggcgacggc
      541 agtggcggcc tcgcggagca cggtgatgat ctcaccggcc cattgctgct gggtggggtg
      601 cagttccagg acgcctttgg cgtggcggat taggtgcgct gcgcattgct ggacgccgtc
      661 gagttggtcg tcgaactggt gccagccggc gtagtcgtcg cggaccaggt agccggtgta
      721 gccggccagg acacccaggt ctgcgatggc gtttttggat cgggagccga tcggcgcgta
      781 ccacacgagc cgcgcgtcgg gggtgcgtac ggtgaccgcg tgcggggatc cgggtaccgg
      841 tgtgccgtgg gcgtcggtgt tgttgtggat cacgttggtg ggggtttcat cggcgcagag
      901 cacgtcctgg gcgcgcagtg cggttgtcat ggcgtcatcg aacccggcca cggccaggcg
      961 ctgggcgaag cgttgcaggg cgcgtgccac aaacccgctc gacaccgggg cacccagcag
     1021 cgcctccatc agcatcgcgg tccgctccag gggcacattg ccctcgctgg ccaacaggat
     1081 cgccgcggca ttgatgttcg gcccgtaggt cacggtgccg gccgtcccga acggtggtgc
     1141 cgcggtggtc gtgtgcccgc agccacagcg gcgccgcggg agcaggtagt gcaccttttc
     1201 catcatgatc ggcaggatgt cccacacctg cgcccagctc atccccacat ccacggcatc
     1261 ggtcagatcc ccgccacacc ccgagcacgc acccggcggg ggcagtgtct gggtccggtc
     1321 cggcgtcacc gccggcgcca gccccgagcc cttgtggcct ggctgcccac cgggcttgcg
     1381 atccttcgag cgctcccgcg atgagcgatc cgcccgccgc gctgccttgg ccccgatcga
     1441 gtccttcgat ggcggcgtcg aggaattcgt cgagttcatc cccagccgcc gccgcaactc
     1501 agcgttttcc gcctccaagg tctccacccg cgccaccaac cccaccacca gcgcggcaag
     1561 ctcctcatac gaaggctcag gcggttcggg cgcagacaca acccagatga tcaacgatca
     1621 cgcaccccac aacgaacccg acacgccgtc accgaccatc aacaccccac caagatccac
     1681 tcacgggcga cggcccagct caaccggacc cgtgaatgga cacgtatcgc cggggcaggg
     1741 tcgacggcga cgcagcccgt tggcgtaggt ttgcccgaac ttggcgcacc attgccggat
     1801 ggtttcgtgg gagaccacga t
//
LOCUS       DATADN010001242         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_144725, whole genome shotgun sequence.
ACCESSION   DATADN010001242 DATADN010000000
VERSION     DATADN010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144725"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..392)
                     /locus_tag="VHH53_15935"
                     /pseudo
     CDS             complement(253..392)
                     /locus_tag="VHH53_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603653.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            617..1699
                     /locus_tag="VHH53_15940"
     CDS             617..1699
                     /locus_tag="VHH53_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2291635.1"
                     /translation="MSQAAQPSQAHTDSEEVIVGVDTHKDVHVAAVISVLGVLLGTAS
                     FPTTAVGYRQLLAWVGGFGTLRRAGVECTGSYGAALSRCLLAAGLEVFEVNQLDKAGR
                     RRRGKTDAIDAEAAARAVLSGQATALAKTSNGPVEMVRMFRLARASAIKSRTQAINQL
                     KAVLVCADPMLRESLSGLSNTQLIRQCAQLPAQAPSDITTAAVYTLQLLARRILDLTH
                     QINDLKQRITQVLAAHAPQLLDHYGVGPDTASVLLIAAADNPERLHSEASFAALCGVS
                     PVEASSGKAQRRRLNRGGDRQANSALYSIVLSRLRWDARSRNYVQRRIAEGKTPREAI
                     RCLKRYVTREIYQIITSPPETQPSAA"
     gene            2014..2985
                     /locus_tag="VHH53_15945"
     CDS             2014..2985
                     /locus_tag="VHH53_15945"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEX2291636.1"
                     /translation="MLMLVDREEISRGLVEGLKYKEIAGRIRRDPSVISREVVRHGGR
                     HDYRAAAADEAACVARQRPKWFAVQRSLRLRAVVCRQLRGGWSPASIAGRLSTDYPDD
                     QACRVSHEAIYQWVYAQPVSTLARELISLRTGRRARSGGRRPPAAPRIREPRYLDQRP
                     AEATDRTLPGHWEGDLVIGKDGRSAVATLVERTSRFLLLVPLTGRDSLTVGDAVIAAT
                     GGLPAAIRRSLTWDCGAEMARHATITATGLPVFFAHPHSPWERGSNENLNRIVREFFP
                     KGVTITSDPNYLAMVASEINDRPRKIHNWKKPSELFTELVEAHASTG"
BASE COUNT          617 a         1122 c         1022 g          599 t
ORIGIN      
        1 atcgccgtag gcgacccgga tcccaccgcg aaccctcacg atacgaccca cgagtctccg
       61 acgtgcggct ttgaatggac tgacgaaagt tcacgaaccc tgatcctgac ctggacctgc
      121 actcaaaagc taggacacca aggccagcac ctcgccggca cgggcgacaa atgggtcgct
      181 gccgtgcacc cacagtaact cgcgatggcc tcgggacacc actgactcca acaccgcaac
      241 gaatcggcgg acgtgctgcc aggagcgtgc cgcatcgatc agcagcgaag tgaaccgctg
      301 cacccagcta tagacggtca catgatcgac ctggatcccg cgttcgccca gcaactctca
      361 acatcacggt atgactgtga ggatctccgg tgccgtcccg ggccggggtc tgacccgcgg
      421 tatgactgac cagggtgtct tgggtctgat ctgtcgacct cggcttgagg cggtgcttgc
      481 tgtcgccgga ccggcgtggg gtgtcccgat aggggccttg ccgctaacca gggcaccatc
      541 acagtcctga ccgcccgcgt cggcccggtg atggtcatcc gttccccgag caagcagacg
      601 agcgaccgtg cccaggatgt cccaggcagc ccagcctagc caggcccaca ccgattctga
      661 ggaggtcatt gttggtgtgg acacccacaa agatgttcat gtcgcagcgg tgatcagcgt
      721 gctgggtgtg ttactgggca ctgctagctt cccgacgact gctgttggtt accggcagtt
      781 gctcgcctgg gtaggaggtt tcgggacatt gcgccgggcc ggggtggagt gcactggctc
      841 ctacggggca gcgttgagca ggtgtctgct ggccgcgggg cttgaggtct ttgaagtcaa
      901 ccagcttgac aaggccggcc gtcgacggcg gggcaagacc gacgccatcg acgccgaggc
      961 tgccgctcgg gcggtgttgt ccgggcaagc cactgcactg gcgaagacca gtaatggacc
     1021 ggtggagatg gtgcgcatgt tcaggttggc cagggcatcg gcgatcaagt cgcgcaccca
     1081 ggcaattaat cagcttaagg cggtgctcgt ctgtgctgat cccatgctgc gcgagtcctt
     1141 gtccggactg agcaacactc agctcattcg ccagtgcgcc caactgcccg cccaggcccc
     1201 cagcgacatc actaccgccg cggtgtacac gttgcagctg ctagcccgcc gcatccttga
     1261 cctcacccat cagatcaatg acctgaagca acggatcact caggtgctcg ccgctcacgc
     1321 cccgcagctc ctcgatcact acggggtagg acccgacacc gcgtccgtat tactcatcgc
     1381 cgctgcagac aaccccgaac gactgcacag cgaagcctcc ttcgccgccc tgtgcggtgt
     1441 cagtcctgtc gaggcgtcct cgggcaaggc ccagcgccgt cgactcaatc ggggcgggga
     1501 tcgacaagca aactccgcct tgtactccat cgttttgtcc cggctgcgct gggacgcccg
     1561 cagccgcaac tacgttcaac gacgcatcgc tgaaggcaag acccctcgcg aagcgatccg
     1621 ctgcctaaag cgctacgtca cccgcgagat ctaccagatc atcacttccc cacccgaaac
     1681 acagccctca gcagcttgac atccataggg gcatcagccc gaagcgcaga taccatcgaa
     1741 ccgccaccat gatgacatcg ctgaaccgtg tagggacagg tgtggcatcc cgtcgacgtc
     1801 tcccgttgag accattgccc tactggttac gacacgccga aacgttcacc cctttaaaag
     1861 gattctgcct aaacctctgt ttctcctcag cccagtccgc gatcgccgca tacttcacgg
     1921 ctgttccttc gcgaaccagg ccgccctggg ctcagccagt cgtagcattt gatcttggga
     1981 gttcggtggg acaggattcc cgccgtgcgc gagatgttga tgttagtgga tcgggaggag
     2041 atctcccgtg gtctggtcga gggcttgaag tacaaggaga tcgcgggtcg gatccggcgt
     2101 gatccgtcgg tgatctctcg ggaggtcgtg cgtcacggcg gccgccacga ctaccgggcg
     2161 gcggccgcgg atgaggcggc gtgtgtggct cgacagcgtc cgaagtggtt cgcggtccag
     2221 cgctcgctgc ggctgcgggc ggtggtgtgc cggcagctgc gtggtggctg gtcgccagcg
     2281 tcgatcgcgg gtcggttgtc gaccgattac ccggacgacc aggcttgccg ggtgtctcac
     2341 gaagcgatct accagtgggt ctacgcccaa ccggtctcca ccctggccag ggaactgatc
     2401 agcctgcgga ccgggcgcag ggcccggtcc ggtgggcgcc gaccgccggc tgccccgcgg
     2461 atccgcgagc cccgctacct cgaccaacgg cccgctgagg cgaccgaccg gacacttccc
     2521 gggcactggg agggggacct cgtcatcggc aaagacggcc gctcggcggt cgcgacgctg
     2581 gtggagcgga cctcacggtt tctcctcctg gtgcccttga ccggccggga ctcgctgacc
     2641 gtcggtgatg ccgtcatcgc cgccaccggt ggactgcccg cggcgatccg ccggtcgctg
     2701 acctgggact gcggcgccga gatggcacgc cacgccacca tcaccgcgac cgggctgccg
     2761 gtgttcttcg cccacccaca ctccccgtgg gaacgcggca gcaacgaaaa cctcaaccgg
     2821 atcgtccgcg aattcttccc caaaggcgtg acgatcacct ccgaccccaa ttatctcgcc
     2881 atggtagctt ccgaaattaa cgaccgaccc cgtaagatac acaactggaa gaaaccctcc
     2941 gagctattca ccgaacttgt cgaggcgcat gcttccactg gctgaatctg ccaggtcctt
     3001 tcgggcgcgt catgcaacgc tcagcccctt ggtgctgctt accagctatc gacgatgaaa
     3061 agtgtgtaca acccgacaaa tacgatgatt gcagagtacg caatccatgc cgctcctcca
     3121 ggccgttcgt cctcgttgcg ctgcgggcgc tgcgcctgga ccccgactgt gggtgccgca
     3181 cacctaaaac acgccggaaa cgaccaaaaa acggtcacaa cgacaacaac cccgccttga
     3241 ccggttccgc cgctttcagg gatgaccccc aggacgtagg gtaggcatct ctagtggtca
     3301 gacgggtgcg gccgtgtgcg tgagcggtcg acgtgcgggt tgttcgcccg cgtgcaggtg
//
LOCUS       DATADN010001243         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_145340, whole genome shotgun sequence.
ACCESSION   DATADN010001243 DATADN010000000
VERSION     DATADN010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145340"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VHH53_15950"
     CDS             <1..1205
                     /locus_tag="VHH53_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2291637.1"
                     /translation="LFSAGTLICALAPGFPVLLVGRVVQACGTAVMIPLLMTTVMTLV
                     PAERRGAMMGNITLVIAVAPAIGPMVGGLVLAALDWRWMFWSVLPLVVAMLALGCARM
                     RLSSATRSAPFDVASVLISAVAFGGIVYGLSAIGAAAQGAGGALPWMPIVVGLIALVV
                     FVARQLRLQRAERALLDLRPFTHRRFVVAVVLSGLVFMCLLGAGAILLPLYLQSVLGA
                     SPRTTGLAVLPGGLVLGLLGRPVGRLFDRFGARPLVVPGSIAMAVSLWLFAALGSGSS
                     LATVIAVHILLMVALGLMMTPLFAGALAALPNTLYSHGSAIMATLQQVAGAAGTAAFV
                     TVAAIASNDPSGAPDATGLRAGFVAAGVVGLVAVGTALFTRGKQTEGPSRDVADAGTS
                     ETPAPVAG"
     gene            complement(1202..>2204)
                     /locus_tag="VHH53_15955"
     CDS             complement(1202..>2204)
                     /locus_tag="VHH53_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291638.1"
                     /translation="PPGPAEHCPTGAEFAEQLLRPLAELPELATMIRSHTRVLAVARQ
                     GLLKHEEIGSKQRGSQPFRLLLAGPRDGIDNVEYVEHADLLLDCTGSYGQANCLGDGG
                     IPAPGELQVADRIIRRIPDLDSEADEWAGRRVLLVGAGKSAQTAARDLAELVVAQPGT
                     QVLWAVRAADPDWGKVSNDPLPQRQELVRVSQRLRSGAVPGVQMRTAVVIDALRETDD
                     GVAVTLRSSAGDVEEIIVDRVIALTGFLGDTSLYRQLQVHECYATAAPMNLSAALLGA
                     SSAGQADCLAQASHGVDTLRVPEPNFFVLGMKSYGRNSTFLLRVGYEQVDEVANAYS"
BASE COUNT          275 a          767 c          769 g          393 t
ORIGIN      
        1 cgcttttcag tgccggcacg ctgatctgcg cgctcgcgcc gggcttcccg gtgctgctcg
       61 tggggcgggt tgtccaggcc tgcgggacgg cggtgatgat tccgctgctg atgaccacgg
      121 tgatgacgct ggtcccggcc gagcggcgcg gcgccatgat gggcaacatc acgctcgtca
      181 tcgcggtcgc cccggcgatc gggccgatgg tcgggggcct ggtcctggca gcgctcgact
      241 ggcgatggat gttctggagc gtcctcccgc tcgtcgtcgc catgctcgcc ctggggtgtg
      301 cccggatgcg cctgagctcg gccacccgct cggcgccttt cgacgtcgcc tccgtgctga
      361 tctccgcggt ggcgttcggc ggcattgtgt acggcttgtc ggccatcggt gcggccgccc
      421 agggcgccgg gggtgcgctc ccgtggatgc cgatcgtcgt gggcctgatc gcgctcgtgg
      481 tgttcgtggc gcgccagttg cgcctccagc gggcggaacg agccctgctc gatctgcggc
      541 ctttcaccca ccgccggttc gtggtggctg tcgtgctctc cgggctggtc ttcatgtgcc
      601 tgctcggagc cggtgccatc ctgttgccgc tctacctgca gagcgtgctc ggcgccagcc
      661 cgcgcaccac cggcctcgcg gtactgccgg gaggtctcgt gctgggtttg ctcgggcgac
      721 cggtcggacg gctcttcgac cggttcgggg cgcgcccgct ggtcgtccca gggtcgatcg
      781 cgatggcggt ctcgctgtgg cttttcgccg cgctgggctc cggttcctcg ctggccacgg
      841 tgatcgcggt gcacatcctg ctcatggttg cgctcggtct gatgatgacg ccgctgttcg
      901 ccggggccct cgccgcgctc ccgaacacgc tgtactcgca cggcagcgcc atcatggcca
      961 cgttgcagca ggtcgctggg gccgccggca ccgcggcctt cgtgaccgtc gccgcgatcg
     1021 cctcgaacga cccgtcgggt gccccggacg ccaccgggct ccgggcgggg ttcgtcgccg
     1081 cgggagtcgt cgggctcgtg gccgtcggga cggcgctgtt cacccgcggc aagcagaccg
     1141 agggtccgag ccgggacgtg gcagacgccg ggacgagcga gacaccggcg ccggtcgccg
     1201 gctaggagta ggcgttggcc acctcatcga cctgctcgta gccgacgcgg agcagaaagg
     1261 tgctgttacg tccataggac ttcatgccca gtacgaagaa gttcggctcc ggtacccgca
     1321 gcgtgtccac cccgtgcgac gcctgtgcca agcagtccgc ctggccggca ctcgacgcgc
     1381 ccagcaacgc cgccgacagg ttcatcggtg cggcggtggc gtaacactcg tgtacctgga
     1441 gctgccggta cagcgaggtg tcacccagga aaccggtcaa ggcgatcacg cggtcgacga
     1501 tgatctcctc gacatcccca gcgctcgacc ggagagtgac ggccacaccg tcgtccgttt
     1561 cgcgcaacgc gtcgatcacc acagcagttc gcatctggac tccgggtacc gcgccggacc
     1621 gcagccgctg cgacaccctg accagctctt ggcgttgggg tagaggatca ttggagacct
     1681 tcccccagtc cggatcggcc gcccgtactg cccacagcac ctgggtgcct ggctgtgcga
     1741 cgaccagctc agcgaggtcc cgtgctgcgg tctgggctga cttgccggcg ccgaccagca
     1801 gaacccggcg acctgcccac tcatcggcct cgctgtccag atccggaatc cggcggatta
     1861 tccgatccgc aacctgcagt tccccaggag ccgggatgcc gccgtcgccg aggcagttgg
     1921 cctggccgta gctgccggtg cagtccagca gcagatcggc gtgctccacg tattcgacgt
     1981 tgtcgatgcc gtctctgggg ccggcgagca gtagccggaa gggctgcgag ccgcgctgct
     2041 tactaccgat ctcctcgtgc ttgagcaggc cttgccgggc aacggcgagc accctggtgt
     2101 ggctacgaat catggtcgcc agctccggta gctcggccag ggggcgaagc aactgctcgg
     2161 cgaattcagc gccggtcggg cagtgctcgg caggccctgg cggc
//
LOCUS       DATADN010001244         5200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_145522, whole genome shotgun sequence.
ACCESSION   DATADN010001244 DATADN010000000
VERSION     DATADN010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145522"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VHH53_15960"
     CDS             complement(<1..901)
                     /locus_tag="VHH53_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX2291639.1"
                     /translation="MTKGTTEQRYRIERDTMGEIAVPADALYRAQTQRAVANFPISGR
                     GLERSQIRALGLVKAACARVNGQLGMLDPTLAEAIASAADEVAAGEHDSHFPVDVFQT
                     GSGTSSNMNANEVIATLASRAAGVDVHPNDHVNASQSSNDVFPTTIHLAATEAVAHHV
                     IPALVHLASALTRKAQDWRNVVKAGRTHLMDAVPITLGQEAGGWASQARHGAERLASC
                     LPRLGELPIGGTAVGTGLNTPAGFGAAVVEQLRRSTGLDVLTEARDHIEAQAARDGLV
                     ECSGALRTVAVSLFKIANDIRWLG"
     gene            complement(926..1966)
                     /gene="glpX"
                     /locus_tag="VHH53_15965"
     CDS             complement(926..1966)
                     /gene="glpX"
                     /locus_tag="VHH53_15965"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX2291640.1"
                     /translation="MSTLSTPRQNLQREEPDRNLAMELVRVTEAAAMAAGRWVGRGDK
                     NGGDQAAVDAMRKLVGSVSMRGVVVIGEGEKDEAPMLFNGEEVGNGQGPECDVAVDPI
                     DGTTLMSKGMPNALAVLAVSERGSMFDPTAVFYMEKIATGPEAAEVIDLSVPVAENIK
                     RIAKAKRMEISDITVCILDRPRHDDLVCQVREVGARITFITDGDVAGAIAAARPDTGV
                     DLLMGIGGTPEGIISAAALRCMGGAIQGRLWPRDDTERQAALDAGHDLDRILTTEDLV
                     RGDNVFFCATGITDGELLRGVTYRAGGCSTQSIVMRSKSGTVRMIDSFHQLAKLRAYA
                     SVDFGQLPPIRK"
     gene            complement(2010..2261)
                     /locus_tag="VHH53_15970"
     CDS             complement(2010..2261)
                     /locus_tag="VHH53_15970"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX2291641.1"
                     /translation="MSRTDDPAMLGYEAARDELTEVVRALEAGGLTLEDSLALWERGQ
                     QLAAVCERHLAGARERIESVITAREQRGAPPLTEPESGS"
     gene            complement(2269..3498)
                     /gene="xseA"
                     /locus_tag="VHH53_15975"
     CDS             complement(2269..3498)
                     /gene="xseA"
                     /locus_tag="VHH53_15975"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX2291642.1"
                     /translation="MTDPATTPEAPWPVRTVARKIAAWVDRLGAVWVEGQITQVSARA
                     GTGTAFLTLRDPVADVSLTLTCPVALVRDRQPPLADGSRVVVHGKPSVHLGRGTLSLR
                     VDEIRAVGIGELLARIERLRRLLDAEGLFDPRRKRRLPLLPGCIGLITGRASAAERDV
                     LSNATARWPAVHFRVQNVAVQGPLAVGQIIDALTVLDADPAVDVIVLARGGGSVEDLL
                     PFSDEALCRAVSGCRTPVVSAIGHEPDTPLVDHVADKRCSTPTDAGKCVVPDVTEEST
                     RLGQLRDRCRRALHGWVDRERQLLDALRSRPVLANPTRPLDERGRLVTDLRAQADRAV
                     LGGVRAAQERLEHTVARLTTLGPAATLARGYAVVQRVDGPVQGHVLRSVAGAPEGTGL
                     RVRLSDGALHAVVTGGR"
     gene            complement(3509..4180)
                     /locus_tag="VHH53_15980"
     CDS             complement(3509..4180)
                     /locus_tag="VHH53_15980"
                     /inference="COORDINATES: protein motif:HMM:NF033649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid droplet-associated protein"
                     /protein_id="HEX2291643.1"
                     /translation="MSTLPWPVRIAVGLAATAVEQAHKLPELPVTAASRAVQAAMRVQ
                     QRVTELAIKGDEVFAMFQPVEEAPPWARFDEDDDPGARGTRNGTTGAAREFGQVPGGA
                     APTAAAPRPGTGHGTPAGASGEIDEADVARAMTPAPSEVADAQPRNAAPAEPADASVA
                     RSAIDPPPVLPHYDDMSIAQLRAKLRTLSLPELTELLEYEHAHQDRAAFVTMLSNRIS
                     TVRSR"
     gene            4199..5149
                     /locus_tag="VHH53_15985"
     CDS             4199..5149
                     /locus_tag="VHH53_15985"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX2291644.1"
                     /translation="MTKRVLLAKPRGYCAGVDRAVVTVEKALEAHGPPVYVRKQIVHN
                     RHVVETLQDRGVIFVDEADEVPAGAIVVFSAHGVAPAVHEQAAARGLRTIDATCPLVT
                     KVHQEAKRFARNGYDILLIGHEGHEEVEGTTGEAPLQIQLVDGPDAVDSVTVRDPSRV
                     VWLSQTTLSVDETMQTVAALKKRFPELADPPSDDICYATQNRQVAVKAMAADCDLVLV
                     IGSRNSSNSVRLVEVALATGAGAAYLVDYAREIDSAWLQGMHTVGVTSGASVPEVLVR
                     DVLDFLAEHGYTDVQEITTAEEKLVFSLPRELKRSMPATP"
BASE COUNT          799 a         1785 c         1763 g          853 t
ORIGIN      
        1 agcccagcca ccggatgtcg ttggcgatct tgaacagcga gacggcaacg gtacgcagcg
       61 cgccggagca ctcgaccagt ccgtcccggg cggcctgcgc ctcgatgtga tcacgcgcct
      121 cggtgagcac gtccaggccg gtggaacggc gcagctgctc gacgacggcc gcaccgaagc
      181 ccgccggggt gttgagcccg gtgcccaccg cggtgccgcc gatgggcagc tcgcctagcc
      241 ggggcaggca gctcgccagc cgctcggcgc cgtgccgagc ctgtgatgcc cagccacctg
      301 cctcttgacc gagcgtgatc ggcaccgcat ccatcaggtg cgtgcggccg gccttcacca
      361 cgttccgcca gtcctgtgcc ttccgggtca gggccgatgc caggtgcacc agcgcgggta
      421 tcacgtgatg cgcgacggcc tcagtggcgg cgaggtggat cgtggtcggg aacacgtcgt
      481 tggaggactg cgacgcgttg acatgatcgt tggggtgcac gtcgaccccg gcggcccggc
      541 tcgccagcgt ggcgatcacc tcattggcgt tcatgttcga actggtgccc gagccggtct
      601 ggaatacgtc aaccgggaag tgggagtcat gctcgccggc cgcgacctcg tcggcggccg
      661 acgcgatcgc ttcggcgagt gtggggtcga gcatcccaag ctgcccgttc acccgcgcac
      721 aagcggcctt gaccaggcca agagcgcgga tctgggaccg ttccaggccg cgtccggaga
      781 tcgggaagtt ggcgacggcg cgctgggtct gcgcgcggta cagcgcatcg gccggcaccg
      841 caatctcgcc catggtgtcg cgttcgattc ggtaccgctg ctcggtggtg cctttggtca
      901 tatcggcagt gtgagggtcg gtcgactact tcctgatcgg gggaagctgg ccgaagtcca
      961 ccgaggcgta cgcgcgcagc ttggccagct ggtggaagct gtcgatcatc cgcacagtgc
     1021 cggacttgga acgcatcacg atggactgcg tgctgcaccc acctgcgcga taggtcaccc
     1081 cccgcagcag ctccccgtcg gtgataccgg tcgcgcaaaa gaacacgttg tcgccgcgga
     1141 ccaagtcctc ggtggtgaga atccggtcga ggtcgtgccc ggcgtccagc gccgcctggc
     1201 gctcggtgtc gtctcgcggc caaagccggc cctggatcgc gcctcccatg cagcgcagcg
     1261 cagccgcgct gataataccc tccggtgttc ccccgatgcc catcagcaga tccacgcccg
     1321 tgtccgggcg agccgcggcg atcgcgccgg cgacgtcgcc atcggtgatg aaagtgatcc
     1381 gggcgccgac ttcgcggacc tggcatacca ggtcgtcgtg tcgcggccgg tccagaatgc
     1441 acacggtgat gtcggagatc tccatccgct tggccttggc gatccgcttg atgttctccg
     1501 caaccggcac ggacaggtcg atcacctcgg cagcctccgg accggtggcg atcttctcca
     1561 tgtagaacac cgcggtggga tcaaacatcg agccccgctc ggacaccgca agcacagcca
     1621 gagcgttggg catgcccttg ctcatcagcg tggtgccgtc gattgggtcg accgcgacat
     1681 cgcattccgg gccctgcccg ttgccgacct cctcaccgtt gaaaagcatc ggcgcttcgt
     1741 ctttttcgcc ctcgccgatg accacgacgc cgcgcatcga taccgagccg accagcttgc
     1801 gcatcgcgtc cactgccgcc tggtcgccac cgtttttgtc accgcggccg acccaacggc
     1861 cagcagccat cgccgcagcc tcggtgactc gcaccagttc catggcgagg ttgcggtcag
     1921 gttcctcgcg ctgaaggttc tgacgaggag tcgacaaagt actcatgggc cctcccggag
     1981 ttgactgcgc aacactggcg atcctgccat caggacccgg attcgggctc cgtcaatggt
     2041 ggtgcaccac gctgctcgcg agcagtgatc acggactcga ttcgctcccg ggcaccggcg
     2101 aggtgccgct cacacactgc ggcgagttgc tggccgcgct cccacagcgc cagggagtcc
     2161 tccaacgtca gcccgcctgc ctccagggcg cgcaccactt cagtcagttc atcccgggcg
     2221 gcttcgtagc ccagcatggc tggatcgtcc gtgcggctca ccgggtcatc atcggccacc
     2281 ggtcaccacc gcgtgcaggg ccccatcgga cagccgcacc ctcagtccgg tgccctcggg
     2341 cgcgccggcc accgaccgca gcacgtgacc ttgcactggc ccatccaccc gctggacgac
     2401 tgcgtaaccc cgggcgaggg tggctgccgg tcctagggtg gtcaggcggg caacagtgtg
     2461 ctcgagccgc tcctgggcgg ctcgtacccc accgagtacg gcccggtccg cctgagcacg
     2521 caggtcggtc acaagccggc cgcgctcatc cagtggacgc gtcgggttgg ccaacaccgg
     2581 acggctgcgc agtgcgtcaa gtagctgccg ctcccggtcc acccagccat ggagggcacg
     2641 ccggcagcgg tcgcgcagct gtcctagtcg agtggattcc tcagtgacgt cgggcaccac
     2701 gcatttgccg gcatctgtcg gcgtggagca gcgcttgtcc gccacgtggt ccaccagcgg
     2761 agtgtccggc tcgtggccga tcgcggagac cacgggggtt cggcagcccg atactgcgcg
     2821 gcacagcgcc tcatcggaga acggcagcag gtcctccacg ctaccgccgc ctcgagccag
     2881 cacgatgacg tccaccgcag ggtctgcgtc gagcacagtg agcgcgtcga tgatctggcc
     2941 aaccgcgagc gggccctgca ccgccacgtt ctgcactctg aaatgcacag cgggccagcg
     3001 ggcggtggcg ttgctgagca cgtcacgctc ggcggcgctg gcccggccag tgatcagccc
     3061 gatgcaaccg ggcagcaacg ggagccggcg tttgcggcgc ggatcgaaca agccttcggc
     3121 gtcaagcaac cgccggagcc gctcgatgcg ggccagcagc tcgccgatgc cgactgcgcg
     3181 gatctcgtcg actcgcaagc tcagcgtgcc acggccgagg tgcaccgacg gcttgccgtg
     3241 caccacgacg cggctgccgt cggccagtgg cggctgcctg tcacgtacca gcgcaaccgg
     3301 gcaggtcagg gtcaacgaga cgtcggcgac cgggtcgcgc aaggtgagaa acgctgtacc
     3361 ggtccctgcc ctggccgata cctgggtgat ctgcccttca acccagaccg cgcccagcct
     3421 gtcgacccat gccgcgatct tgcgggctac cgtgcggacc ggccagggcg cttctggcgt
     3481 ggtggccgga tccgtcacag gcatctggtc atcgagagcg gaccgtggag atccggttgg
     3541 acagcatggt gacgaatgcc gcgcggtcct ggtgcgcgtg ctcgtactcc agcagttcgg
     3601 tgagctcggg tagcgacagc gttcgcagct tggcgcgtag ctgggctatc gacatgtcgt
     3661 cgtagtgcgg taacaccggt ggcgggtcga tcgcggaccg ggccacgctg gcgtctgcag
     3721 gttcggcggg tgcggcgttc ctgggttggg cgtcggctac ctccgagggc gcaggtgtca
     3781 tggcgcgggc gacatcggcc tcatcgatct cgccggacgc acccgccggg gtgccgtgtc
     3841 cggtgcccgg acggggggct gccgcggtgg gcgctgcgcc gccggggacc tgaccgaact
     3901 cgcgtgctgc tcctgtggtg ccgtttctgg tgccacgcgc accgggatcg tcgtcttcat
     3961 cgaaccgggc ccacggtgga gcctcttcca ccggctggaa catcgcgaat acctcgtcac
     4021 ccttgatggc cagttcggtg acccgctgct gcacccgcat cgcggcctgc acggcgcggc
     4081 tggccgcggt gacaggtagc tcggggagct tgtgagcctg ttccacggcg gtggccgcca
     4141 gacccaccgc gatccgcacg ggccacggca aagtgctcat ggtagttagg gtagaggcat
     4201 gaccaagcgt gtgctgctcg ccaagccccg cgggtactgt gccggggtcg accgcgcggt
     4261 ggtgaccgtc gagaaggccc tcgaggcgca cgggccgccg gtgtacgtgc gtaagcagat
     4321 cgtgcacaac cggcacgtcg tggagacctt gcaggaccgc ggggtgatct tcgtcgacga
     4381 ggccgacgag gtgcctgccg gtgccatcgt ggtgttctcc gcgcacggag tcgccccagc
     4441 ggtgcacgag caagccgctg cgcgcggcct gcgcaccatc gacgccacct gcccactggt
     4501 gaccaaggtg caccaggagg ccaagcggtt cgcccgcaac ggctacgaca tcctgctgat
     4561 cggtcacgag ggccacgagg aggtcgaggg caccaccggt gaggcgcccc tgcagatcca
     4621 actcgtggac ggccctgacg cggtggattc ggtaaccgtg cgcgatccat cccgggtggt
     4681 gtggctgtcc cagaccaccc tgtcggtaga cgagaccatg cagacggtgg ctgcgcttaa
     4741 gaagcgcttt cccgagctgg ccgacccccc cagcgacgac atctgctatg ccacgcagaa
     4801 ccggcaggtg gcggtgaagg cgatggccgc cgactgcgat ctggttctgg tcatcggatc
     4861 gcggaactcc tccaactcag tgcggttggt cgaggttgcg ctggccaccg gcgccggggc
     4921 cgcctacctg gtcgactatg cacgggagat cgactcggcc tggctgcagg gcatgcacac
     4981 cgtcggcgtc accagtggtg cttcggtgcc cgaagtcttg gtccgtgacg tactcgactt
     5041 ccttgccgag catggataca ccgacgtgca ggagatcacc actgccgagg agaagctggt
     5101 gttctcgctg ccgcgggaac tcaagcgctc gatgccagca actccctgac ccccggtaag
     5161 cgctcaggtg gtgcggcgca cactcggtcg tcgctgagtg
//
LOCUS       DATADN010001245         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_146107, whole genome shotgun sequence.
ACCESSION   DATADN010001245 DATADN010000000
VERSION     DATADN010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146107"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="cofH"
                     /locus_tag="VHH53_15990"
     CDS             <1..1137
                     /gene="cofH"
                     /locus_tag="VHH53_15990"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:TIGR03551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX2291645.1"
                     /translation="PAPARATGAVGAVLDGVRTGQRPGLDELVTLLSARGAEVAAVAE
                     MADELRATTVGDTVTYVVNRNINYTNVCTFRCRFCGFSKGKLSLNLRGAPYLLELPEI
                     VARVLEAQRCGATEVCLQGGIHPSFDGDYYLTVCRAIREAAPTIHIHGFTALEVTEGA
                     RRLGEPLADYLIRLREAGLRSLPGTAAEILDDEVRAVLCPDKITTAEWLEAHRVAHSV
                     GLRSNITMMFGAIEQPVHWARHLLRAHALQEQTGGFTEFVGLPFVHMATPMYLKRAAR
                     RGPTWREVLLVHAVARIAFHGLIDHVQASWVKLGTSGARQLLQAGVDDLGGTLMDENI
                     SRAAGASHGQSLDEAGFRAIVEPLGRPLAQRTTLYEPVATVQAP"
     gene            complement(1255..>1878)
                     /locus_tag="VHH53_15995"
     CDS             complement(1255..>1878)
                     /locus_tag="VHH53_15995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2291646.1"
                     /translation="AARAEERAQWTAAGIVPGARVADIGCGPGTVLRLLAEEVGNGGR
                     ADGVDLDAGAVTAAAEQVADLTQASVRVGEAAATGLDPGSYDVVMCRHVLAHNGGHEA
                     AITAHLIELARPGGAVISSTSTAPRCGRIPKTPTLPISTPGTQKCTALAATTSPLARR
                     SGPCSKTRVAGRVFPQRGAGAPASTAAAPAVLGRSGCAGGRPARRPG"
BASE COUNT          287 a          650 c          658 g          283 t
ORIGIN      
        1 cccgcgccgg cacgtgcgac cggcgcggtg ggtgcggtgc tcgacggtgt ccggaccggg
       61 cagcgtcccg gcctcgacga gctggtgacg ctgctgtccg cccgtggtgc cgaggtcgcg
      121 gctgttgccg agatggccga cgagctgcgc gccacaactg tcggcgacac cgtcacttac
      181 gtggtcaatc gcaacatcaa ctacaccaac gtgtgcacct tccggtgccg gttctgcggg
      241 ttctccaaag gcaagctgtc actgaacctg cgcggcgcgc cctacctgct cgagctgcct
      301 gagatcgtgg cgcgggtact tgaggcgcaa cggtgtggcg ccaccgaggt atgcctgcag
      361 ggtggcattc accccagctt cgacggtgac tactacctga cggtctgccg ggcaatccgg
      421 gaggccgcgc ccacgatcca catccacggc ttcaccgcac tggaggtcac cgaaggagcc
      481 cggcggctgg gtgaaccact cgccgactac ctgatccggt tgcgcgaggc gggcctgcgc
      541 tcgctgcccg ggaccgccgc ggagatcctc gacgacgagg tccgggcagt actgtgcccc
      601 gacaagatca ccactgcgga gtggctggag gcgcaccggg tggcgcactc ggtggggttg
      661 cgatccaaca tcacgatgat gttcggcgcc atcgagcagc ctgtgcactg ggcccggcac
      721 ctgctgcgcg cccacgccct acaggagcag accggtgggt tcaccgagtt cgtcgggctg
      781 cccttcgtgc acatggccac accgatgtac ctcaagcggg ccgcccggcg cggaccgacg
      841 tggcgggagg tgctgctggt gcacgcggtg gcccggatcg cattccacgg gttgatcgac
      901 cacgtgcagg cgtcctgggt caagctcggc acctccggtg cacggcagct gctgcaggct
      961 ggagtggacg atctcggcgg cacactgatg gacgagaaca tcagccgcgc agctggcgcc
     1021 agccacggcc aaagcttgga cgaggccgga ttccgcgcga tcgtcgagcc cctcgggcgg
     1081 ccgctggcgc agcgcaccac cctctatgag ccagtcgcta cggtgcaagc gccgtaactg
     1141 gcgacgccat caagggccat gcttacggcc cacggccatg aagaatgcga gggtcatcca
     1201 gggccgggtg ggagcgcgtc gacacgctcg aatgcggccg tccacctgcg cagatcagcc
     1261 ggggcggcga gccgggcggc caccagcgca tcccgagcgg cccaggacgg cgggcgcagc
     1321 tgcggtggaa gctggtgcac cggccccccg ctgcggaaag actcgacctg caacccgcgt
     1381 tttcgagcag ggtcccgagc gtcgggccaa tggcgatgtt gttgccgcga gcgcggtgca
     1441 tttctgcgta ccgggcgttg agatcggcaa tgtcggggtt ttcgggatgc gtccgcatcg
     1501 cggtgccgtc gatgtcgacg agatgaccgc cccaccggga cgcgcgagct cgatgagatg
     1561 ggcggtgatc gctgcctcat ggccgccgtt gtgggccagg acgtggcggc acatcaccac
     1621 gtcatagctg cccgggtcga gtccggtggc cgcggcctcg ccgacccgca cggaagcctg
     1681 ggtgagatcg gcgacctgct cggcggcggc ggtgacggcg ccggcatcca ggtcgacacc
     1741 atctgccctc ccgccgttgc caacctcctc ggcgagcaac cgcaggaccg ttcctggccc
     1801 acagccgatg tctgccaccc gcgcgccggg cacgatcccg gcagccgtcc actgggctcg
     1861 ctcctcggca cgggccgc
//
LOCUS       DATADN010001246         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_1356593, whole genome shotgun sequence.
ACCESSION   DATADN010001246 DATADN010000000
VERSION     DATADN010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356593"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..664
                     /locus_tag="VHH53_16000"
     CDS             218..664
                     /locus_tag="VHH53_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2291647.1"
                     /translation="MRAADLVSPYPTVRSDTPVLEAARLLAGQNLPGLIVVDDRGAPF
                     TILPGTEVLRLAVPGYCQDDPTLARVVDEAAADVFLRGLGERTVAESLPKKRRELPVV
                     EPHATVLEMAALMARTHTPLVAVVEPDGALQGAITLDALLDRVLAQ"
     gene            661..1962
                     /locus_tag="VHH53_16005"
     CDS             661..1962
                     /locus_tag="VHH53_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX2291648.1"
                     /translation="MTTVAWLAVAIFAAAYVLIATEWIHRVAAALGGAVLMLLIGATD
                     AEHAFFSEESGIDWNVIFLLLGMMLIVAVLQRTGAFDFVAIWAAKRARGRPFRVMVIL
                     VLVTAFASALLDNVTTVLLIAPVTLLVCERLAVPAAPFLIAEALASNIGGTATLVGDP
                     PNIIIASRSGLSYNDFLIHLAPLVVVLMVVFIGLCRWLFRRAFISDPQRAAAVMALRE
                     RDAIRDRRLLVISLVVLAVVTAAFVLHTALNLEPSVVALVGGLVLLAASRLNVEQVAK
                     DVEWPTLVFFAGLFVMVGSLVNTGVIDQLATAAVDATEGRLLLTSQVLLVASAGLSAI
                     VDNIPYVATMSPIVADLVQANGGIGSAQVLWWSLALGADLGGNATAIGASANVVVLGI
                     AERSGQRISFWQFTKYGLVVTAVTIAIAMPYVWLRYFVFAG"
BASE COUNT          300 a          700 c          700 g          421 t
ORIGIN      
        1 caaatcggct ctcgccttgt tcgatgccct tcctagacct gcgggcatcg tacgggctgg
       61 ttggtttctg gaaccaactc cgtaggcgct acgcgccccg ggttctgtgt atcaggcgga
      121 cgagccaagg ctgagagata ccggtacggt ctttcgtggt gtgccgggaa gcctggtcgg
      181 cggattagct gtcgcctagc tgacggggga gcagtcattg cgcgccgccg atcttgtatc
      241 gccctacccc acggtccggt cggatactcc ggtgcttgag gccgcccggc tgctggccgg
      301 gcagaacttg ccagggctga tcgtcgtcga cgatcgcgga gcaccgttca cgatcttgcc
      361 cggcaccgag gtgctgcgct tggcggtgcc cggttactgc caggacgatc ccaccttggc
      421 cagggtggtc gacgaggccg cggcggatgt gttcctgagg ggacttggtg agcgcacggt
      481 cgccgagtcg ctgcccaaga agcgccggga gttaccggta gtggagccgc atgcgacggt
      541 gctggagatg gccgcgctga tggcgcgcac ccacacgccc ttggtggccg tcgtcgagcc
      601 ggacggtgcg ctgcaaggag ccatcacgct tgacgcgctg ctggaccgag tgctcgcgca
      661 gtgaccacgg ttgcctggct ggcggtggcc atcttcgccg ccgcctatgt gctgatcgcc
      721 accgagtgga tccatcgggt cgccgccgcg ctgggtggcg cggtgctgat gttgctgatc
      781 ggcgctaccg acgccgagca cgccttcttc tccgaagagt ccggcatcga ctggaacgtc
      841 atcttcctgc tgctcgggat gatgctcatc gtcgccgtgc tgcagcgcac tggggcgttc
      901 gatttcgtgg cgatctgggc agccaaacgg gcccgcggcc ggccgttccg ggtgatggtg
      961 atccttgtcc tggtcaccgc gttcgcgtcg gctttgctgg acaatgtgac caccgtgttg
     1021 ctgatcgcgc cggtgaccct gctggtctgt gagcggttag cggtaccggc ggcaccgttc
     1081 ctgatcgccg aggcactcgc gtcgaacatc ggtggcaccg ccaccctggt cggcgatcca
     1141 ccgaacatca tcatcgccag ccgctccggg ctgtcctaca acgactttct catccacctg
     1201 gccccgctgg tcgtggtgtt gatggtggta ttcatcgggt tgtgccggtg gcttttccgg
     1261 cgcgcgttca tctccgaccc gcagcgcgcc gcggcggtca tggccttgcg cgagcgcgac
     1321 gcgatccgcg atcgccgcct gctcgtcatc agcctggttg tgctcgccgt ggtcaccgcc
     1381 gcgttcgtac tgcacaccgc gctgaacctg gagccctcgg tagtggcgct cgtcggtggg
     1441 ctcgtgctgc tcgccgcgtc gcggctcaac gtcgaacagg tcgccaagga cgtcgagtgg
     1501 cccaccctgg tgttcttcgc cggcctgttc gtcatggtcg gttcgctggt caacaccggc
     1561 gtgatcgacc agctggcgac cgcggctgtt gatgccactg aagggcggct gctgctgacc
     1621 tcgcaggtgc tgctggtggc ctccgctgga ctgtccgcga tcgtggacaa catcccttac
     1681 gtggccacca tgagcccgat agtcgctgat ctcgtccaag ccaacggcgg catcggctcc
     1741 gctcaggtgc tgtggtggtc gctggcatta ggtgctgacc tcggtggcaa cgccaccgcg
     1801 atcggtgcct cagccaacgt ggtggtgctc ggcatcgctg aacgatccgg gcagcggatc
     1861 tccttctggc aattcaccaa gtacgggctg gttgtcaccg cagtcaccat cgccatcgcc
     1921 atgccctacg tctggctgcg ctacttcgtc ttcgccggct aagccccttg atgagcagca
     1981 gcgccaggct ccctcagagg gcaagcaccg cgatgatcaa gccgtagatg ccgatcagga
     2041 gcaccccctc gaaaccgacg ctagcccaac cgcgggcctg ccggacaagc aggccggcca
     2101 cgagcaccac ggacgtcaac a
//
LOCUS       DATADN010001247         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_147397, whole genome shotgun sequence.
ACCESSION   DATADN010001247 DATADN010000000
VERSION     DATADN010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147397"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VHH53_16010"
     CDS             complement(<1..809)
                     /locus_tag="VHH53_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX2291649.1"
                     /translation="MTTSVALPWAAPRAPGPVRATVTVPGSKSITNRALLLTALAGTG
                     GIVRGALRSRDTDLMVGALHALGVAVTSCDGQMEVAAHNGLRGPATVDCGLAGTVMRF
                     VPPLAALADGPVRFDGDERARQRPMGAVLGALRALGCRVDGDALPFVLHGRGALPGGE
                     VRIDASGSSQFVSGLLLSAAGYDAGITVHHDGPPVPSLPHIEMTVQMLADRGVDVDTD
                     DATWWRVAPGPIAGGDHDVEPDLTNAAVFLAAAAVTGGEVTVPGWPRQTTQA"
     gene            849..1607
                     /locus_tag="VHH53_16015"
     CDS             849..1607
                     /locus_tag="VHH53_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX2291650.1"
                     /translation="MCGRYASTRDPATLAVEFDALDATGGIAPEADYNVAPTKPVLSV
                     VTRHPRDVQGIPDPDRTVRSIRVMRWGLVPHWAKDPSVGSRMINARAESAAGKPAFRD
                     AVARRRCLLPADGWYEWQAQPGSRKQPIFVTPADGSGVALAGLWSTWRGCDDTAPALV
                     TCAVVTTEAVGPLAEIHDRMPLILPARAWERWLDPDSDDPSELLAPPAAELVHALELR
                     PVSTAVNDVRRGGHELVERVQPEQPALLDLDQVQ"
     gene            1604..2248
                     /locus_tag="VHH53_16020"
     CDS             1604..2248
                     /locus_tag="VHH53_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX2291651.1"
                     /translation="MTGLTIPTPHGTAEVELRWGQAAAPRGYPHAIAAVLLGHGAGGS
                     TGAPDLVAVTNAATGIGIHVALVTQPYRVAGRRVPAPARQLDQAWLAVAAHLAKGVFT
                     GLPIVFGGRSSGARVACRTAAAGAAVAVLCLAFPMHPPRRPERSRIQELEGVEVPVLV
                     VQGQRDPFGRPQPATGREVVLLPGDHSLKADPGAVGSAVAQWLQRRLADGGAQS"
BASE COUNT          326 a          776 c          822 g          330 t
ORIGIN      
        1 gcctgagtgg tttgccgtgg ccagcccggc acggtgacct ccccgccggt gaccgcggcg
       61 gcggccagga acaccgccgc gttggtcagg tccggctcga cgtcatgatc gccgcccgcg
      121 atggggccag gggccactcg ccaccacgtg gcgtcatcgg tgtccacgtc gacgccccgg
      181 tcggccagca tctgcacggt catctcgatg tgcggcaggg aggggaccgg cgggccgtcg
      241 tgatggaccg tgatgccggc gtcgtagccg gcggcggaca gcagcaatcc ggagacgaac
      301 tgtgaggagc ccgacgcgtc gatccgcacc tcgccaccgg gcaacgcccc gcggccgtgc
      361 agcacgaatg gcagtgcgtc gccgtcgacg cggcaaccca gcgcgcgcag cgccccgagc
      421 accgcgccca tcggccgctg ccgggctcgc tcgtcaccgt cgaaacggac cgggccatcg
      481 gccagcgcgg ccagcggcgg gacgaagcgc atgacggtgc ctgccagacc gcagtccacg
      541 gtggcggggc cgcgcagccc gttgtgtgct gctacctcca tctggccgtc gcagctggtg
      601 accgctacgc cgagggcatg cagggcgccg accatgagat cggtgtcccg gctgcgcagc
      661 gctccccgga cgatgccacc ggtgccggcc agcgcagtca gcagcagtgc ccggttggtg
      721 atcgacttcg atcctgggac cgtgacggtg gcccgcaccg ggcccggcgc ccgtggcgcg
      781 gcccacggca atgcaacgga ggtcgtcatg gcaccatcat cccgtacccg catgccacga
      841 tggcgcctat gtgcggcagg tacgcctcga ccagagatcc cgcgacgctg gccgtcgagt
      901 tcgacgcgct ggacgctacc ggcggtatag cgccggaagc cgactacaac gtcgcgccga
      961 ccaaaccggt gctgtcggtg gtaacccggc atccgcgcga tgtgcaagga atccccgatc
     1021 cggaccgcac agtgcgcagc atccgggtga tgcggtgggg cctggtgccg cattgggcca
     1081 aggatcccag cgtcgggtcc cgaatgatca acgcgcgagc ggagagtgcc gccggcaagc
     1141 ccgccttccg tgatgcggtg gccaggcgcc gctgcttgct gccggccgac ggctggtacg
     1201 agtggcaggc tcagcccggg agccgcaagc agccaatctt cgtcacccct gcggatggct
     1261 ccggtgtcgc cctggccggg ttgtggtcca cctggcgggg ctgcgacgac accgcacctg
     1321 cgctggtgac ctgcgcggtg gtcactaccg aagcggttgg tccgctcgcc gagatccacg
     1381 accggatgcc gctgatcctg cccgcccggg cttgggagag atggctggat ccggactccg
     1441 acgatcccag cgagttgctc gcaccaccgg ctgccgagct ggtgcacgcg ctggagctgc
     1501 ggccggtgtc aacagcggtg aacgacgtcc gccgcggcgg tcacgagctg gtcgagcgag
     1561 tgcagccgga gcagcccgcg ttgctggatc tggatcaggt gcagtgacgg gactgaccat
     1621 tccgacaccc cacggcacag ctgaagttga gctgcgctgg ggtcaagcag cagctccgcg
     1681 gggatacccg cacgccatag cggcggtgct gctggggcat ggcgcgggcg gttccaccgg
     1741 cgcgcccgac ctggttgcgg tcaccaatgc ggcgaccggt atcgggatac acgtggcgct
     1801 ggtcacgcag ccctatcggg tcgcgggccg gcgtgtccca gcgccagctc ggcagcttga
     1861 ccaagcgtgg ctggccgtcg ctgcgcacct ggcgaagggc gtcttcaccg gtcttccgat
     1921 cgtcttcggg ggccggtcct cgggcgcgag ggtggcgtgt cgcactgcgg cggcgggagc
     1981 ggccgtggcc gtgctgtgct tggccttccc gatgcacccc ccaaggcgcc cggaacgcag
     2041 ccggatccag gagttggagg gcgtggaggt gcctgtgttg gtcgtgcagg gacagcggga
     2101 tccattcggt cgccctcagc ccgccaccgg gcgggaggtc gtgctgctgc caggagacca
     2161 ttcactcaag gctgatcccg gcgcggtcgg cagcgcggtc gcgcagtggc tgcagcgtcg
     2221 gctagctgat ggtggtgccc agagttaatc aatc
//
LOCUS       DATADN010001248         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_148251, whole genome shotgun sequence.
ACCESSION   DATADN010001248 DATADN010000000
VERSION     DATADN010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148251"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..337)
                     /locus_tag="VHH53_16025"
     CDS             complement(11..337)
                     /locus_tag="VHH53_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291652.1"
                     /translation="MLTARDRDPLLPAPTPADENGVAARKVRLNRLRARLSQAWFAHN
                     VQKPTAAELEEAQHHAEHELAVHEDGHQHVVGDYTGEYELDGQPADGHQFDGRHGVAG
                     EELRQH"
     gene            complement(377..1801)
                     /locus_tag="VHH53_16030"
     CDS             complement(377..1801)
                     /locus_tag="VHH53_16030"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEX2291653.1"
                     /translation="MNVDTSKVATTNATTPATGRKSGRIGAVATWADDRLGLGSVAKK
                     QLRKVFPDHWSFMLGEIALWSFVVLLLTGTFLTLWFKPSMAEVVYDGSYDQLRGLHMS
                     EAYASTLDISFDVRGGLLMRQMHHWAAMLFIAAMFVHMMRIFFTGAFRKPREVNWMIG
                     GLLLMLGILEGFAGYSLPDDLLSGTGLRIADGLMKSVPVLGTYVSFFAFGGEFPGDLI
                     IPRLYVVHILLIPGILLALIGAHMLLLVYQKHTQWPGPGRTNDNVVGYPMMPVYAAKA
                     GGYFFIVFGVTALMGGLLGINPVWKYGPYNPAEVTAGSQPDWYMGIAEGLLRIMPGWE
                     TEIFGFTISWNIMIPGVVAPGIIFGVVLLYPFIEAWITGDKRDHHLLQRPRNAPTRTA
                     FLVAMMTLYGLLWAAGGNDILAVIFNLNLNYITYFMRVMIFVGPVIAFIITRRWCISL
                     QRQDEARLLHGYETGIIMRSPEGA"
     gene            complement(1798..>1940)
                     /locus_tag="VHH53_16035"
     CDS             complement(1798..>1940)
                     /locus_tag="VHH53_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinol-cytochrome C reductase"
                     /protein_id="HEX2291654.1"
                     /translation="SAKVVFGPAARPLPQLPLSVDSEGYLVAQRDFTEPVGPSYWERD
                     SR"
BASE COUNT          326 a          608 c          695 g          311 t
ORIGIN      
        1 gcctgaggcg tcagtgctgg cgcagctcct cgccggccac cccgtggcgg ccgtcgaact
       61 ggtgcccgtc ggcgggctgt ccgtcgagct cgtactcacc ggtgtagtca ccgacgacgt
      121 gctggtgacc gtcctcgtgg accgcaagct cgtgctcggc gtggtgctgg gcctcctcga
      181 gctcggccgc ggtcggcttc tgcacgttgt gggcgaacca agcctgggac aggcgggccc
      241 ggagccggtt gagccggacc ttgcgggcag ccacgccgtt ctcgtcggcc ggggtcggtg
      301 ccggcagcag cgggtcacgg tcgcgggcgg tcagcacgta ggcctcgtca ccggagatcg
      361 gcaggtgctt ctcggcttac gcgccctcgg gggagcgcat gatgatgccg gtctcgtagc
      421 cgtgcagcag tctcgcctcg tcctgacgct ggagcgagat gcaccagcgt cgcgtgatga
      481 tgaaggcgat caccgggccg acgaagatca tcacccgcat gaagtacgtg atgtagttca
      541 ggttcaggtt gaagatcacg gccaggatgt cgttgccgcc cgcggcccac agcaggccgt
      601 agagcgtcat catcgcgacc aggaacgccg tgcgggtcgg cgcgttgcgc gggcgctgga
      661 gcaggtggtg atcgcgcttg tcaccggtga tccaggcctc gatgaacggg tagagcagca
      721 caaccccgaa gatgatgccc ggtgcgacga cgcccgggat catgatgttc cagctgatcg
      781 tgaacccgaa gatctcggtc tcccagccgg gcatgatgcg cagcaggccc tcggcgatgc
      841 ccatgtacca gtcgggctgg gagccggcgg tgacctcggc cgggttgtac gggccgtact
      901 tccacaccgg gttgatgccg aggaggccgc ccatcagcgc ggtcacgccg aagactatga
      961 agaagtagcc gccggccttg gcggcgtaca ccggcatcat cgggtaaccg acgacgttgt
     1021 cgttggtgcg gccggggccg ggccactgcg tgtgcttctg gtacacgagg agcagcatgt
     1081 gggcgccgat gagcgccagc aggatgccgg ggatcagcag gatgtggacg acgtacagcc
     1141 gggggatgat caggtcaccc gggaactccc caccgaaggc gaagaaggac acgtaggtgc
     1201 cgagcaccgg caccgacttc atcaggccgt cggcgatgcg caggccggtg ccggagagca
     1261 ggtcgtcggg cagcgagtag ccggcgaagc cctccaggat gccgagcatc agcagcaggc
     1321 cgccgatcat ccagttgacc tcgcgcggct tgcggaacgc gccggtgaag aagatgcgca
     1381 tcatgtgcac gaacatcgcc gcgatgaaca gcatcgccgc ccagtggtgc atctgccgca
     1441 tcagcaggcc gccgcggacg tcgaaggaga tgtcgagcgt ggaggcgtag gcctccgaca
     1501 tgtgcaggcc gcggagctgg tcgtaggagc cgtcgtagac gacctcggcc atgctcggct
     1561 tgaaccacag cgtcaggaag gtgccggtga gcagcagcac cacgaagctc cacagcgcga
     1621 tctcacccag catgaacgac cagtggtcgg ggaagacctt gcgcagctgc ttcttggcga
     1681 cagatccgag ccccagccgg tcgtcggccc aggtcgccac ggcgccgatc cggccgctct
     1741 tcctgccggt ggcgggggtg gtggcgttgg tggtcgcgac cttgctggtg tcgacgttca
     1801 tcgtgagtca cgctcccaat agctgggtcc gaccggttcg gtgaagtctc gctgggccac
     1861 gaggtatccc tccgagtcta ctgacaaggg cagctgcggc agcggccttg ctgccgggcc
     1921 gaagaccacc ttggcggagt
//
LOCUS       DATADN010001249         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_148759, whole genome shotgun sequence.
ACCESSION   DATADN010001249 DATADN010000000
VERSION     DATADN010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148759"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VHH53_16040"
     CDS             complement(<1..281)
                     /locus_tag="VHH53_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2291655.1"
                     /translation="MSSDASASTTGTAQIGVTGLAVMGSNIARNFARHGYTVALHNRS
                     VAKTDALLEQHGSEGKFVRSETISEFLDALEKPRRVLIMVKAGEPTDAV"
     gene            complement(338..1258)
                     /locus_tag="VHH53_16045"
     CDS             complement(338..1258)
                     /locus_tag="VHH53_16045"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX2291656.1"
                     /translation="MSALAFTVMALVLSGPVPAVLARASWPFRAPRAAIVLWQAIALA
                     AVLSAFSAGIAIASRLFAPGRDGRPTTTITSEIAVLGWPVWIAYVVVFALTLVIGARL
                     IAAVLQVAIATRRRRAHHRMVVDLVGKSQKSGLRILDVDQPLAYCLPGVRSRVVVSEG
                     TLTTLADKEIAAILSHERAHLRARHDLVLEMFTAVRAAFPRFVRSASALDAVRLLIEM
                     LADDAAVRIAGPTPLARALVACAAGRTPSGALAAGGPTTVLRLHRLGGEPNSLALAAC
                     AYSAAAAVLVVPTIAVVLPWLTELQRLFAT"
     gene            complement(1273..1689)
                     /locus_tag="VHH53_16050"
     CDS             complement(1273..1689)
                     /locus_tag="VHH53_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX2291657.1"
                     /translation="MAKLTRLGELERAVMDHLWSEREPQTVRQVHEALSAHRDLAYTT
                     IMTELQRLAKKNLVVQHRDDRAHRYAPTHGRDELVAGLMVDALDQAADSGSREAALVH
                     FVERVGADEAAALRRALAELESKHGVAPPAGNPGTG"
     gene            1906..>2242
                     /locus_tag="VHH53_16055"
     CDS             1906..>2242
                     /locus_tag="VHH53_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2291658.1"
                     /translation="MTVLVDDPLIASMAIRRTLPLHESSRRLRELYPECPRVYGVAVM
                     GDLSRRRWWPLAEALTTDRLRSMFDLAAEETDSRAAVAHQLAATLAHVVVGRVVPLVV
                     LEGRAWDTGL"
BASE COUNT          359 a          738 c          772 g          373 t
ORIGIN      
        1 atcaccgcgt cggtgggttc tccggccttg accatgatca gcacccgacg gggcttctcc
       61 aacgcgtcga ggaactccga gatggtctcg ctgcgcacga atttgccttc ggagccgtgt
      121 tgttcgagca acgcgtcggt cttggcaacc gagcggttgt gcagggcgac ggtgtatccg
      181 tgccgggcga agttccgggc gatgttcgat cccatcacgg caaggccggt gaccccgatc
      241 tgagcggtac cggtggtcga agcggaagcg tcagagctca ttcgggcagc ctttcgttgt
      301 tttgttgtgg gaacgcttcg tggggcgcga ggtgtcccta agtggcgaac aggcgttgca
      361 gttcggtcag ccacggcagg accaccgcga tggtcggcac caccagcacc gcggctgcgg
      421 ccgagtacgc gcacgcggcc agcgccagac tgttgggctc accgccgaga cgatgcaacc
      481 gcagcaccgt ggtcggcccg cccgcggcca gggcacccga tggggtgcgg ccggccgcgc
      541 aggcgaccag ggcacgggcc aggggagtgg gaccggcaat gcgcactgcc gcgtcgtcgg
      601 ccagcatctc gatcagcaga cgcaccgcgt ccagtgcgct tgcgctgcgg acgaagcgcg
      661 gaaacgccgc gcgcaccgcg gtgaacatct cgagcaccag atcgtgccgg gcccgcaggt
      721 gcgctcgttc gtgggagagg atggcggcaa tctccttgtc cgccaacgtg gtcaacgtgc
      781 cttcgctgac cacgacgcgg ctgcgtaccc ccggcaggca gtaggccagc ggctggtcca
      841 cgtcgaggat gcgcagccca cttttctgcg acttgcccac caggtccacg accatccggt
      901 gatgagcgcg tcggcgccgt gtcgcgatcg cgacctgcag caccgcggcg atcaatcgcg
      961 caccgatgac caatgtcagc gcgaaaacca ccacgtaggc gatccagacc ggccaaccca
     1021 gaaccgctat ctcgctggtg attgtcgtgg tcggtctgcc gtcgcgaccc ggggcgaaca
     1081 ggcgactcgc gatcgcgatg ccggccgaga aggccgacag caccgccgcc agagcgatcg
     1141 cctgccacag cacgatggcc gcccggggtg cacggaaggg ccaggatgcg cgggccagca
     1201 ctgctggcac cggtccggac aggaccagcg ccatgacggt gaaggccagc gcggacacgc
     1261 tgtaagtctc cctcagccgg tgcccggatt gccagcgggc ggcgcgacgc cgtgcttgct
     1321 ctccaattcg gccagtgcgc gacgcaacgc cgcggcttcg tcggcgccga cccgctcgac
     1381 gaagtgcacc agtgccgcct cccggctgcc ggaatcggcg gcctggtcga gcgcgtcgac
     1441 catcaggccc gcgaccagct cgtcgcggcc gtgggtcggc gcatagcggt gggcgcgatc
     1501 atcgcggtgc tgcacgacga ggttcttctt cgccagtcgt tgcaactcgg tcatgatcgt
     1561 ggtgtaggcc aagtcgcgat gggctgagag cgcttcgtga acttggcgca cggtttgggg
     1621 ctcgcgctca gaccacaagt ggtccatcac tgcgcgttcc agttccccta gacgcgtcaa
     1681 cttcgccatg gtcagttcat ctcctagagg ccgtggatcc agcttactac tggttactac
     1741 cgtgtgtcgt atatcgcacg gcggacagcc ggcagatcgc gccaaactcc actccggccg
     1801 tggtcaccgc ccgttctcgg gcagtgtgaa agcagtcaca gggtctagcc gtcctggtgc
     1861 gtctagctat agtaaggctt acctaactcg atattgggag gccccatgac ggttctggtc
     1921 gacgatcccc tcatcgcgag catggcgatc cgcaggacgc taccgctgca tgagtcgagt
     1981 cggcggctgc gcgagctcta ccccgagtgt ccgcgggtgt acggcgtcgc ggtgatgggc
     2041 gacttgtccc ggcggcggtg gtggccgttg gccgaggcgc tgacgaccga ccgcttgcgg
     2101 tccatgttcg acctggcggc cgaggagacc gacagccgcg cggcggtggc ccatcagttg
     2161 gcggcgacgc tcgctcatgt cgtggtagga cgcgtcgtgc cgctcgtggt gctcgaaggg
     2221 cgggcatggg acaccggcct gg
//
LOCUS       DATADN010001250         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudonocardiaceae bacterium isolate SMAG_U6751
            k141_148989, whole genome shotgun sequence.
ACCESSION   DATADN010001250 DATADN010000000
VERSION     DATADN010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955373
            Sequence Read Archive: SRR12676716
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardiaceae bacterium (soil metagenome)
  ORGANISM  Pseudonocardiaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 12:33:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,213
            CDSs (total)                      :: 3,186
            Genes (coding)                    :: 3,058
            CDSs (with protein)               :: 3,058
            Genes (RNA)                       :: 27
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 25
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 45 of 128
            Pseudo Genes (incomplete)         :: 84 of 128
            Pseudo Genes (internal stop)      :: 23 of 128
            Pseudo Genes (multiple problems)  :: 22 of 128
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Pseudonocardiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148989"
                     /isolate="SMAG_U6751"
                     /isolation_source="soil"
                     /db_xref="taxon:1873463"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="33.37 N 114.19 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VHH53_16060"
     CDS             complement(<1..777)
                     /locus_tag="VHH53_16060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX2291659.1"
                     /translation="MTTTGSLPPGAAVGSFTVEALIGRGGMGEVYRAVHRELGRPVAL
                     KLLLPALAMQEEFAARFVREARMMQSLDHPCIVTVFDAGDADGRLYLAMRLVTGISLK
                     ERLAEGLLPPEQIVSILEQLAEALDYAHSRGVIHRDIKPSNILIDPRNQPVLVDFGLA
                     KALGDVSVTVSSRYLGTPTYMAPEQAAGDPVSHRADLYSLACVAFEMLTGTPPYTEQD
                     SLALLLAHGTRPVPRATHRDPQLPAGVDAVFERALANSPTD"
     gene            complement(774..1397)
                     /locus_tag="VHH53_16065"
     CDS             complement(774..1397)
                     /locus_tag="VHH53_16065"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2291660.1"
                     /translation="MARWRWAGMTAYLEVWKPSGVELVLLETRRVTIGRNSSNDVPLS
                     HDKTVSGLHAVLEPVGGGWCVQDVGSRNGTFVRGERILGMCALRHGDDLAVGRTKLVF
                     RAAEPAGTTRTEGSAPPPELTRRERDVLRALCRPLVSGQVFTEPASLHEIATTLVVTE
                     AAVQQHLLRLYDKFGIAPGTGSRRGQLANEAIRRGAVPLADLWDSPP"
BASE COUNT          237 a          551 c          539 g          226 t
ORIGIN      
        1 gtcggtgggg gagttggcca gtgcacgctc gaagacggcg tccacccccg cgggcagctg
       61 cggatctcgg tgggtggcgc gcggcaccgg ccgggtgccg tgggccagca gcagcgccag
      121 ggagtcctgc tcggtgtagg gcggggtgcc ggtgagcatc tcgaaggcca cgcacgccag
      181 ggagtacagg tcggcccggt ggctgaccgg gtcaccggcg gcctgctcgg gtgccatgta
      241 cgtcggcgtc cccaggtacc ggctgctgac ggtgacggag acgtcgccga gggccttggc
      301 taggccgaag tcgaccagta ccggctggtt acgcgggtcg atcaggatgt tggacggctt
      361 gatgtcgcgg tggatcacgc cgcggctgtg cgcatagtcc agcgcctcgg cgagctgctc
      421 gagtatcgag acgatctgct cgggcgggag caggccctcg gcgagccgtt ccttgaggct
      481 gatcccggtg accaggcgca tcgccaggta gagccgtccg tcggcgtctc cggcgtcgaa
      541 caccgtgacg atgcagggat ggtcgaggct ctgcatcatc cgagcctccc ggacgaaccg
      601 ggcggcgaac tcctcctgca tggccagcgc cgggagcagc agcttgagtg ctaccgggcg
      661 acccagttca cggtggaccg cacggtagac ctcacccatt ccgccccgac cgatgagcgc
      721 ctcgacggtg aacgagccga ccgcagcacc ggggggcagc gatccggtgg tggtcatggc
      781 ggcgagtccc acagatcggc aagggggacc gcgccgcggc gaatggcctc gttggccaac
      841 tggcctcggc ggctgcccgt gccgggggcg atgccgaact tgtcgtagag ccgcagcagg
      901 tgctgctgta ccgcggcctc ggtgacgacc aatgtggtgg cgatctcgtg cagggacgcc
      961 ggctcggtga acacctgacc actgaccagg ggccgacaca gcgcgcgcag cacatcacgc
     1021 tcgcgacggg tcagctccgg cggcggcgct gacccctcgg ttcgtgtggt ccctgctggc
     1081 tccgccgctc ggaacaccaa cttcgtccgc cccaccgcaa ggtcatcacc gtgccggagg
     1141 gcgcacatcc ccaggatgcg ctccccccgc acgaacgtgc cgttgcggct accgacgtcc
     1201 tgcacgcacc agcccccgcc gaccggctcc aggactgcat gcaggccgga gaccgtcttg
     1261 tcgtgggaga gggggacgtc attgctgctg ttgcggccga tcgtcacccg gcgcgtctcc
     1321 agcagcacga gctcgacacc cgagggcttc cacacctcca gatacgccgt catgcccgcc
     1381 caccgccacc tggccatggc tcccggctcc gcaccggcga tcccctggcc agcccccaga
     1441 cccagcgtga cgacatggca agtctccctg gagacactac taccagcccc ggctggccga
     1501 gcctaccgac ctgacccgtc accccgtccc gatcctcggc cgagaagtgg gcc
//
